LOCUS       DASPLT010000001         1571 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_20485, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000001 DASPLT010000000
VERSION     DASPLT010000001.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1571)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1571)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1571
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_20485"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..205)
                     /locus_tag="VEA63_00005"
     CDS             complement(<1..205)
                     /locus_tag="VEA63_00005"
                     /inference="COORDINATES: protein motif:HMM:NF025597.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4339 domain-containing protein"
                     /protein_id="HYD82386.1"
                     /translation="MFTIIGGDGQEYGPATAEQLRAWIAAGRANLDTQARVAGSDEWR
                     RLGDYPEFSTLDAPPLVTTPATVV"
     gene            complement(334..639)
                     /locus_tag="VEA63_00010"
     CDS             complement(334..639)
                     /locus_tag="VEA63_00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbY family RNA-binding protein"
                     /protein_id="HYD82387.1"
                     /translation="MYDLPLTGAQRSLLRARGQTLEPALKLGKGGLTPAFFAELQQHL
                     RARELVKLRFLGFDRDERAELCTRIADEGRCLCVGAVGHTALFYRQHPEPAERTIAL"
     gene            863..>1571
                     /locus_tag="VEA63_00015"
     CDS             863..>1571
                     /locus_tag="VEA63_00015"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HYD82388.1"
                     /translation="MNTHLPAAFAVSTPLRPSASRVPAAGAWRYRAPRRTRAAAWGAV
                     LFSAGVHAVILFAFNRKAAPKAAVGVEEAFAVQLVMPDLKDLEEPEVEPFEGDEEPVD
                     AGISVPMLADTPAFVDLTNAFLQEIDYSSLVPQQDLTGAKTLSIPTNIHRGGKIGEGM
                     GKVFDLKDLDRAPEPLVRIAPVVPVGMRQQGFKAEVVVGFIVDANGAVTGAYVVRSSD
                     RNLEESALLAMGKWKFRP"
BASE COUNT          270 a          424 c          608 g          269 t
ORIGIN      
        1 tgacgacggt tgcgggtgtc gtgacgagcg gcggagcgtc gagcgtggaa aattccgggt
       61 agtcgccgag gcggcgccat tcgtcggagc cggcaaccct ggcctgcgta tccaaatttg
      121 cgcgaccggc ggcgatccat gcgcgaagtt gttcggctgt ggccggtccg tattcctgcc
      181 cgtcgccccc gatgatcgtg aacatggcgg aacgaaagag tcgggcggcg gcgccgtcaa
      241 ccggcggttc ggagcggacg ccggcgtgtg ggtgaatgag cgtgaaggca agtcgcgcgg
      301 ctcgccgggg acggcttgcc ctacctcgcg cgtttagagc gcgatggtgc gttcggcggg
      361 ctcggggtgt tggcggtaga acaaggcggt atggccgacg gcgccgacgc agaggcagcg
      421 gccttcgtcg gcgatgcgcg tgcagagctc ggcgcgttcg tcgcggtcga agccgaggaa
      481 gcgcagcttc acgagttcgc gggcgcgcag gtgttgttgc aattcggcga agaacgccgg
      541 cgtaagaccg cctttgccga gcttgagcgc gggttcgagc gtttggccgc gagcgcggag
      601 caacgagcgc tgggcgccgg tgagcgggag gtcatacatg gcggaaaaag tctcggaagc
      661 ggcgatctcg caaaggccgt ggacggctcg cggtaagcgc ggattttggt gaagaggcag
      721 cgtcggcgag ttgggggcga acgccaagga tggattcgaa gaaacggcgg agaagcgggc
      781 gaggtgtcga cgtcacaacg aatgcgcggg atttgccaaa tcgtgcggag gaagccgtcg
      841 caaaccggac gatgattagt ccatgaacac gcacctccct gcggctttcg ccgtttcgac
      901 tccactcagg ccttcggctt cgcgcgtgcc ggcggccggc gcctggcgct atcgggcacc
      961 gcgacgaacg cgggcggcgg cgtggggcgc cgtgctgttt tcggcggggg ttcacgcggt
     1021 gatcctgttt gcgttcaatc gcaaggccgc gccgaaagcg gcggtcgggg tggaggaggc
     1081 gtttgccgtg caactcgtca tgccggactt gaaggatctc gaggagccgg aggtggagcc
     1141 gttcgaaggc gatgaggagc cggtcgacgc aggtatcagc gtgccgatgc tggcggacac
     1201 gcccgcgttc gtcgatttga cgaacgcgtt tctgcaggag atcgactact cgtcgctcgt
     1261 gccgcagcag gatctcacgg gtgcgaaaac gctttcgatc ccgacgaaca ttcatcgcgg
     1321 cgggaagatc ggcgaaggca tgggaaaggt tttcgatctg aaggatctcg accgcgcacc
     1381 ggagccgctg gtgcggattg cgccggtggt gccggtgggc atgcgccagc aaggattcaa
     1441 ggcggaggtg gtggtcggat tcatcgtgga tgcaaatggc gccgtgacgg gggcgtatgt
     1501 ggtccgcagc tcggaccgaa atctcgagga gtcggcgctg ctcgcgatgg gtaaatggaa
     1561 gttccggcca g
//
LOCUS       DASPLT010000002         1553 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_102418, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000002 DASPLT010000000
VERSION     DASPLT010000002.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1553)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1553)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1553
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_102418"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            360..>1553
                     /locus_tag="VEA63_00020"
     CDS             360..>1553
                     /locus_tag="VEA63_00020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD82389.1"
                     /translation="MISRSALISAFLLTAIAAAPLRAEPASDLSDAGILGRYEQRRQF
                     ILDQAVAAVVPGDLTKGSKFDIAACLQRGEKVEAAVARLARLDGGEPSGNMFWVYPTV
                     AVMMAGNETLDEASRARIAELWRTYWPSRGDTENHWVLTYASLYLAAQAYPGAGPERW
                     FNGKSSVENMAEAQAYLEHWMKVTTAHGQGEYDSPNYIEEYAVGLSLLAGWAEDPVFR
                     ERARRMLDYIFYDYVVETLDGLYGGAHSRVYPRHIMAPGRTASTALGWLLFGLGEYEP
                     SERQVTRHQLREELPERGAKIIALSGYVPPPILERIARDRSEPYVERELKRTRWRMRH
                     AGSESFVIGDKRTAPVYKTSYVDPDFILGSSQGGLLQPIQQSTWNLIWRTPNAVMDCP
                     TFFGTQ"
BASE COUNT          260 a          507 c          485 g          301 t
ORIGIN      
        1 tgatgggacg acctggtata cacagcggct ctggccggcc cgctactggc agggttggtc
       61 ggactacctc gtgcacacca tccatgcccg tgtgctccag cacatccggg cagaagccga
      121 gcgctcacag taaacctccc gttctcgtcg cttggcgagc gactccgtgc cggcgcgaat
      181 ttcgcgctct cacgcggcgg atgattgcgc cgactccgga cggccacgag gagacccagc
      241 ggcaatgaat attcactcgg atttctcttc cggaatcgtg gcgggccgtt gagctttcgc
      301 cttctcgcga tcgcgtgatt cctcctgtcc ttcctgctcc gcgtccgctt ggcctcccta
      361 tgatttctcg ctctgctctc atctccgcgt ttctcctcac cgcgatcgct gctgcgccgc
      421 tgcgcgcgga gccggcgtcc gatttgtccg acgcgggcat cctcgggcgt tatgagcaaa
      481 ggcggcagtt catcctcgat caggcggtgg ctgccgtggt ccccggcgat ctgacgaagg
      541 gcagcaagtt cgacatcgcg gcctgcctgc agcgcggcga aaaagtcgag gcggcggtcg
      601 ctcggctcgc tcgcctcgat ggtggtgagc cgtccggcaa catgttttgg gtttacccga
      661 cggtggcggt gatgatggcg ggcaacgaaa cgctcgacga agccagtcgc gcgcgtatcg
      721 cggaattgtg gcggacctac tggccgagcc gcggcgatac ggagaatcac tgggtgctca
      781 cctacgcgag tctctacctg gcggcgcagg cctacccggg cgccgggccc gagcgctggt
      841 tcaacggcaa atcctccgtg gaaaacatgg ccgaggcgca ggcctacctc gagcattgga
      901 tgaaggtcac caccgcccac ggccaggggg aatacgactc gccgaactac atcgaggaat
      961 acgcggtcgg cctttcgctg ctggccggct gggcggagga tcccgtgttt cgcgagcgcg
     1021 cgcggcggat gctggattat attttctacg actacgtggt ggaaacgctc gacggcctct
     1081 acggtggcgc gcacagccgc gtttatccac gccacatcat ggctccgggg cgcaccgctt
     1141 ccaccgcgct cggctggctg ctgttcggac tcggggaata tgaacccagc gaacgccagg
     1201 tgacgcgtca ccaactccgc gaggaattgc cggaacgcgg cgcgaagatc atcgcgctca
     1261 gcggctacgt gcctccgccg attctcgagc ggattgcccg cgatcgctcc gagccttacg
     1321 tcgagcgcga actcaaacgc acccgctggc gcatgcggca tgcgggatcg gagtcttttg
     1381 ttatcggcga caagcgaacc gctccggtct ataaaaccag ttacgtcgat ccggatttta
     1441 tcctgggctc gtctcaaggc gggttgctcc aaccgatcca gcagtcgact tggaatctga
     1501 tctggcggac gcccaacgcc gtgatggact gccccacttt cttcggcacg caa
//
LOCUS       DASPLT010000003         1050 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_20513, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000003 DASPLT010000000
VERSION     DASPLT010000003.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1050)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1050)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1050
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_20513"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..379
                     /locus_tag="VEA63_00025"
     CDS             <1..379
                     /locus_tag="VEA63_00025"
                     /inference="COORDINATES: protein motif:HMM:NF023036.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="copper-binding protein"
                     /protein_id="HYD82390.1"
                     /translation="VLVPFAFADTPAKNCGCACCTGKEVCCCHPAESANAKENTASAK
                     EDNGPAAARHALRGVIVDVRPKQSALLVKHETIPGYMRGMTMLFKVDQATLETAKKGQ
                     AITATLVEREDSFWLEDVKSLPQ"
     gene            448..900
                     /locus_tag="VEA63_00030"
     CDS             448..900
                     /locus_tag="VEA63_00030"
                     /inference="COORDINATES: protein motif:HMM:NF022479.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2721 domain-containing protein"
                     /protein_id="HYD82391.1"
                     /translation="MDPLSDDSLASIIQLSISPVILISGVGALTITLTNRMGRIVDRT
                     RSLAGQAHQAPPDERRHLGSQLDIMWRRARLIRFAVTLAGCSMLTACLLILGLFTAAF
                     FELQPGLGLALLFVTSIIFLAAALVAFLRDIFVSLHAVQLEVDRAQGR"
     gene            complement(914..1042)
                     /locus_tag="VEA63_00035"
     CDS             complement(914..1042)
                     /locus_tag="VEA63_00035"
                     /inference="COORDINATES: protein motif:HMM:NF016432.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HYD82392.1"
                     /translation="MSEYEQFSQAEIAAVLGVSVKAVETRLYRAREKLRKALAGWA"
BASE COUNT          191 a          382 c          273 g          204 t
ORIGIN      
        1 cgttctcgtc cccttcgcct tcgccgacac acctgcgaaa aactgcggct gcgcgtgctg
       61 caccggcaaa gaggtctgct gctgccaccc ggccgaatcc gccaacgcaa aagaaaacac
      121 cgcctcggcc aaagaagata acggccccgc ggccgcgcgt catgccctgc gcggtgtgat
      181 cgtggacgtt cgccccaagc agtccgcgct ccttgtgaaa cacgaaacca tccccggcta
      241 catgcgcggg atgacgatgc ttttcaaagt cgatcaagcc acgctcgaaa ccgcgaagaa
      301 aggtcaggcg atcacggcta ccctcgtcga acgcgaagat agtttctggc tcgaagacgt
      361 gaagtccctg ccgcaatagt cgccgcactc cggtgaccgc ctgtttcgcc gccgcccacg
      421 gcaatcgctc cctgcttctc aatcccgatg gacccccttt ccgatgactc gctcgcgtcg
      481 atcatccagc tttcgatttc gcccgtgatc ctcatctccg gcgtcggcgc gctcacgatt
      541 acgctcacga atcgcatggg ccgaatcgtc gatcgcacgc gcagcctcgc cggacaagcg
      601 caccaggcgc cccccgatga acgccggcat ctcggcagcc aactcgacat catgtggcga
      661 cgcgcccgac tcattcgttt cgcggtgacg ctcgccggtt gcagcatgct caccgcgtgc
      721 ttgctcattc tcggactctt caccgccgca tttttcgagc ttcagccggg gctcggcctc
      781 gcgctccttt ttgtgacgag cataatcttc ctcgccgctg cactggtcgc ctttctccgc
      841 gacatcttcg tctcactgca cgccgtccag ctcgaggttg accgggcgca agggcgttga
      901 tggtcctgac tgctcaggcc caacccgcga gcgctttcct caatttctcc cgggcgcgat
      961 aaagccgcgt ctcgaccgcc ttcaccgaga cgccaagcac tgcagcgatc tcagcctgcg
     1021 agaactgttc gtattcagac aagaccagcg
//
LOCUS       DASPLT010000004         4228 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_81946, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000004 DASPLT010000000
VERSION     DASPLT010000004.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 4228)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4228)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4228
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_81946"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..626)
                     /locus_tag="VEA63_00040"
     CDS             complement(<1..626)
                     /locus_tag="VEA63_00040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD82393.1"
                     /translation="MRKRLVFAMLVAAVLLTVGVTTGRRLARGVESANLAPAAETKMD
                     RAGQSPPSVEVAAEAAVVSAVAKSNSAMERVKLYALSEDETALLGATCELQMLAQGTE
                     LELNGDQWSALAAVTMDIQTIRHTYEAEIATVKSVEPGRYRVEVPVYSEAGDALRAQF
                     RDALRAELGDVKATEVIEKMGRRLEGHFAGFGMSVQTLDIVARPESGRT"
     gene            complement(1013..2941)
                     /locus_tag="VEA63_00045"
     CDS             complement(1013..2941)
                     /locus_tag="VEA63_00045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD82394.1"
                     /translation="MALPRIKLIWGGAKQAPGHWRLGIWGVRERQVGSAPRVVVSVRG
                     ILSWMLGLAFAGYLVGATALYVWFERNPYNLVRWTDTLLMPLRWDQVRELRGRMMIAE
                     GLDDLRAKRWGEAHMKLRVGLARAPAETRARLALAEFYNLANRRPDAIKLLAEGLKFG
                     YPGRQYLGSLFGIAAQSEDYETIMTACDTVKAPDEEERAWLLAQKISALISAKRAEEA
                     LALIDADGEPVTAQMREARVLALVETGRSNEAIAFLQEWLEAVPAHHAQVLRLQARAF
                     REAGRTAEMVTALENLRQLAPTDPRTYIYGIVQRFLAGEGESAVEAFGDFVRRFGSSA
                     QSLALASSALAEAKALRLVERCAEVASLHGFPAKTFKLTLAQALIAAGEWRRAGQTLA
                     ELKPVLTDANPTERFSYDSMERVVAIAIQPKGTPESPLLDLLQQRPLPLSVYRHVAEA
                     LLRADRPDAAKSVLAIAERIYPVSATVARLRAEAEAGLETKTTATAAAAVVTLPSTRA
                     AETEEAFFERLNTLEAEGRWREMAEAVRQARVSKPAWLARREAEVFDAQMRTAIQTLD
                     TPELLAAARLYLNGMTERSLRVVDLARGLGVDGRKADGERLIVEVLRKSAGFAPALRL
                     REEWRAADEQAKVATGGE"
     gene            complement(2943..>4228)
                     /locus_tag="VEA63_00050"
     CDS             complement(2943..>4228)
                     /locus_tag="VEA63_00050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HYD82395.1"
                     /translation="TGALERESEEQRGRAAQLAETLVVSQQQVESARSELIELEREIQ
                     ALEGAQSSLHSQSEDAHDQLSAHEIKLAETRQRAQFLAEEVLREYQLEVTTLDWKNLL
                     WRADDEPEGVKPLDLDEDEEGGEESALKAESSELRVESPEQTDQPAAGETEPAQAEAA
                     PVKRRKKAKGPKGEPTEADLAALESTNWDAVKSEADGLRARLGGMGAVNLVAIEEYAE
                     LKQRFDFLKTQSDDLTTAKADLLKAIDEINQTSQKQFEVTFEQIRKNFEYTFHTLFGG
                     GRANIELIATEDILESGIEIVAQPPGTKLKGITLLSGGQKTLTAVALLFALYMVKPSP
                     FCLLDELDAPLDESNIGRFTDLLKKFVGESQFIIITHNKRTVSAASAIYGVTMEERGV
                     SKTVSMKFNAERGDIEAHPQNIAQAVRGAAIPVTA"
BASE COUNT          720 a         1665 c         1050 g          793 t
ORIGIN      
        1 gtccgtccgc tctccggccg cgccacgatg tcgagcgtct gcacgctcat tccgaaaccc
       61 gcaaaatgtc cctcgagtcg gcgccccatt ttctcgatca cctcggtcgc cttcacgtct
      121 cccaactcgg cgcgcagcgc gtcgcgaaac tgagcccgca gcgcgtcacc agcttctgaa
      181 tacaccggca cctcgacgcg atagcgccct ggttcgaccg acttcaccgt cgcgatctcc
      241 gcctcgtagg tgtgccggat cgtctgaata tccatcgtca ccgccgccag cgccgaccat
      301 tggtccccgt tcaactccag ctcggtgccc tgcgccagca tctgcagttc gcatgtcgca
      361 cccagcagcg ccgtctcgtc ctccgacagg gcatataact taactcgctc catcgctgaa
      421 ttggacttcg cgaccgcact caccaccgcc gcctctgccg cgacctcgac cgacggcggc
      481 gactgccccg ctcgatccat ctttgtctca gccgcgggcg ccaagtttgc gctttcaacg
      541 cctcgcgcca gccgacgccc cgtcgtcact ccgaccgtga gcaggaccgc cgccactaac
      601 atcgcaaaca cgaggcgctt tcgcatgcac ccagtgaaac cccggcctaa acgcccgcca
      661 caaaaaactt cgagaaaaag ctaaagtttt cccgggcccg gtcgaccgcc tcgccaacga
      721 ctccagcaac ggcccgcgaa tccaccctgt aaagcggtgt aggtaaaacc gtagccgcgg
      781 ccggccttgt gcctccgagc cgaattccag tttaggtggc gcgctcgtcg cgccacgggg
      841 ttgcgcgcaa gcgcgcaacc cacggaagca cgcaggcgga aacgcctgcc tggcttctat
      901 tcgtgtccct tcgtgtccat tcgtggttca aatcggtttc gttccgatca tccgtgttca
      961 tccgtagttc aacggattga tccctttcat ccgtcgctaa agcgaccgac ttctactccc
     1021 cacccgtcgc caccttcgcc tgctcgtctg ccgcccgcca ctcctcccgc aaacgcaacg
     1081 ccggtgcgaa acccgcggat ttccgcagca cttccacaat cagccgctcg ccgtcagcct
     1141 tccgtccatc aacccccaaa ccgcgggcga gatccacgac gcgcagcgag cgctccgtca
     1201 tgccattgag atacaaccgt gccgcagcca gcagctccgg cgtatccaaa gtttggatcg
     1261 ccgtgcgcat ttgcgcatcg aacacctcgg cctcgcgacg cgcgagccag gccggcttcg
     1321 atacccgagc ctgccgcacc gcttccgcca tttcccgcca acgcccttcc gcctccagcg
     1381 tgtttagccg ctcgaaaaac gcttcctccg tttccgccgc tcgcgtggac ggcaacgtca
     1441 ccaccgcagc cgccgcggtc gccgtcgtct tcgtctccag tcccgcttcc gcctcggcgc
     1501 gcaaccgcgc caccgttgca ctcaccggat aaatccgctc cgcgatcgcg agcacgcttt
     1561 tcgccgcgtc cggccggtcc gcgcgcaaca acgcctccgc cacgtggcga tacaccgaca
     1621 acggcagcgg tcgttgctgc aataaatcca gcaacggcga ctccggcgtc cctttcggct
     1681 gaatcgcgat cgccaccacc cgctccatgc tgtcgtagga aaaacgctcc gtgggattcg
     1741 cgtccgtcag caccggcttc agctcggcca gcgtctgccc cgcgcgccgc cactcacccg
     1801 cagcgatcag cgcctgcgcg agcgtcaatt tgaaggtttt cgccggaaac ccgtgcaaac
     1861 tcgccacctc ggcgcagcgc tcgaccaacc gcagcgcttt cgcttcggcc agcgccgacg
     1921 acgccagcgc gagcgattgc gccgagctgc cgaagcgccg cacaaaatcc ccaaacgcct
     1981 caactgcgct ctcgccttcc cccgccaaaa atcgctgcac gatcccgtaa atgtaggttc
     2041 gcggatccgt cggcgcgagc tgccgcaaat tttccaacgc cgtcaccatc tccgccgtcc
     2101 gccccgcttc gcgaaacgcc cgcgcctgca accgcagcac ttgcgcatga tgcgccggca
     2161 ccgcttccag ccactcctgc aaaaacgcga tcgcctcatt gctgcgaccc gtttcaacca
     2221 acgcgagcac gcgcgcttca cgcatctgcg ccgtcacggg ttcaccgtcc gcatcgatca
     2281 acgccagcgc ttcctccgcc cgtttggccg aaatcaacgc gctgattttc tgcgccaaca
     2341 accacgcacg ctcctcctcg tccggcgcct tcaccgtgtc gcacgccgtc atgatcgttt
     2401 cgtaatcctc gctctgcgcc gcgatcccga acagcgaccc gaggtattga cgccccggat
     2461 acccaaattt caacccttcc gccagcagct tgatcgcgtc cggccggcgg ttcgccaggt
     2521 tgtagaattc cgccagcgcc agccgcgcgc gcgtctccgc cggcgcccgc gccagcccga
     2581 cccgcagctt catgtgcgct tcgccccaac gcttcgcccg cagatcgtcc aagccttccg
     2641 caatcatcat ccgtccgcgc aactccctca cctgatccca acgcagcggc atcagcagcg
     2701 tgtccgtcca ccgcaccaaa ttataaggat tccgctcgaa ccagacatac agcgccgtcg
     2761 cgcccaccag ataacccgcg aacgcgagcc cgagcatcca tgataaaatc cctcgcacgc
     2821 tcaccaccac ccgcggcgcc gagccgacct gccgctcccg cacgccccat attcccaaac
     2881 gccagtgccc cggcgcttgt ttcgcgccgc cccaaatcaa tttgatgcgg ggcagcgcca
     2941 tctcaagcgg tcaccggaat tgccgcaccg cgcacggctt gcgcgatgtt ttgcggatgc
     3001 gcctcgatgt cgccgcgctc ggcgttgaac ttcatggaca cggtcttcga cacgccgcgc
     3061 tcttccatcg tcacgccgta gatcgcgctc gccgccgaca cggtgcgctt gttgtgcgtg
     3121 atgatgatga actgcgactc gcccacgaac ttcttcaaca ggtccgtgaa ccggccgatg
     3181 ttcgattcgt ccaacggtgc gtcgagttcg tccaacaaac agaacggcga tggcttcacc
     3241 atgtagagcg cgaacagcag cgcgaccgcc gtcagcgttt tctgtccgcc cgacaacagc
     3301 gtgatgcctt tcaacttcgt gcccggcggt tgcgccacga tttcgatgcc gctctccaag
     3361 atatcctccg tcgcgatcaa ctcgatgttc gcgcgcccgc cgccgaacag ggtgtggaac
     3421 gtatattcga agttcttccg gatctgctcg aacgtcacct cgaactgctt ctgcgaggtc
     3481 tggttgatct cgtcgatcgc cttcaacaaa tccgctttcg ccgtcgtgag atcatccgac
     3541 tgcgttttca ggaaatcgaa gcgctgcttc aactccgcgt attcctcgat cgcgacgaga
     3601 ttcaccgcgc ccatcccgcc gagccgcgcg cgcaacccgt ccgcctccga cttgacggcg
     3661 tcccagttcg ttgattcgag cgccgccaaa tccgcctccg tcggctcgcc tttcggcccc
     3721 ttcgccttct tccgccgttt caccggcgcc gcctcggctt gcgccggctc ggtttcaccc
     3781 gccgccggct gatcggtttg ctccgggctc tcaactctca gctctgaact ctcagctttc
     3841 agtgcagact cctcgccgcc ctcttcgtcc tcgtccaaat ccaacggctt cacgccttcc
     3901 ggctcgtcgt ccgcccgcca caataaattt ttccaatcga gcgtcgtcac ctcgagctga
     3961 tactcgcgca acacttcctc cgcgaggaac tgcgcccgct gccgcgtctc cgccagcttg
     4021 atctcgtgcg cgctcaactg gtcgtgcgca tcctccgact gcgagtgcag cgacgactgc
     4081 gcgccttcga gcgcctgaat ttcgcgctcc aactcgatca actccgaccg cgcgctctcc
     4141 acctgctgct gcgacaccac gagcgtctcc gcgagctgcg ccgcgcgccc ccgctgctcc
     4201 tcgctctcgc gctccaacgc gccggtct
//
LOCUS       DASPLT010000005         4356 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_184364, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000005 DASPLT010000000
VERSION     DASPLT010000005.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 4356)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4356)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4356
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_184364"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            14..1063
                     /gene="scpB"
                     /locus_tag="VEA63_00055"
     CDS             14..1063
                     /gene="scpB"
                     /locus_tag="VEA63_00055"
                     /inference="COORDINATES: protein motif:HMM:NF016005.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HYD82396.1"
                     /translation="MSLKAKIEAIIYAAEEPITVEQISALVKDMVLAEEAEQARAAAE
                     MAASGEVAEQAEVAEPSASLEPAEAAAGQMDEPAVDGAAETVAGEQSVPEPANGTEIA
                     APAEQPDTIEAGSVTVEGPDATANKKKPAKKELSEDARVRARVRSVIQELASDCAQDD
                     RGIEIRAVAGGFRMSTKPEHHDVVRAFAKSLKPPVRLSLAALETLAVIAYKQPVTVPE
                     ISEVRGVESGGVIGTLLDRKLITTAGRKQVVGRPILYKTSKEFLLRFGLNDIAELPSM
                     EEFEKLASDGQGELFTPEASTEPPHAPAPAAEAPDTSAPETAPDEPVAEEATTAAPEE
                     VSTEPTPQAAEAKTE"
     gene            1293..3014
                     /locus_tag="VEA63_00060"
     CDS             1293..3014
                     /locus_tag="VEA63_00060"
                     /inference="COORDINATES: protein motif:HMM:TIGR00093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HYD82397.1"
                     /translation="MQKVLAAAGIASRRKAEELITEGRVSINGHVVTEMGTKADPRMD
                     EIRVDGQLIGRPERHVYFMVYKPKGHVTTVADPEGRPTVMHLMEGIRERVFPVGRLDY
                     MSEGLLLMTNDGELMAQLTAAKSHVPKTYQVKVSGHPTEEQIDKLRAGVWLPPEPGMT
                     GKRGKKQPGVERRSFGVKTQPAQIELVRDQENPWYEVTLIEGRNRQIRRMFEEIGFHI
                     EKIKRVRYGTLELNVEPGQWRQLTFSEINELKRSLKKPFRIAPPRPRRDTEEPESAPG
                     PRREPRAPRSDARTARGPERPQRSRSERPGYAERPAREGFAARPPRREEGGESRPPRR
                     VSGGFGDRPQRREGGSFQGRPPRREGEGFQSRPPRRESGSFPRRDRESFETRPPRRAS
                     GGFDERPRRERPASGEERPRRERPAFGQDRPRRERPAFGQDRPRRERPAFGEDRPRRE
                     RPQFGEGRPRREGGGFGQQREGGTARERGFADRGARGERPTRNAGPRGERPTGPRGER
                     PSGPRGGRPAGPGGERPSGARGPRREGGEGGGGRGFRREGPPRGDRPQNRRGPGSRSS
                     GPRNRRD"
     gene            3038..4171
                     /gene="hisC"
                     /locus_tag="VEA63_00065"
     CDS             3038..4171
                     /gene="hisC"
                     /locus_tag="VEA63_00065"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HYD82398.1"
                     /translation="MSKFSEFVPERIRQLPPYVPGKPIKQAEAESGVTAIKLASNENP
                     FGPSPLALHAMDDAARSVHLYPDNDATELKNRLAAHHGVGAEQVLVTGGSTPLLDIIA
                     RTVLAPGLNAITSERSFIVYPIVTRSAGAEFRKVPMKNDTFDLDAIAAAVDKNTRLIF
                     LANPNNPTGTIVTAAELDRFLDKLPDHVLVALDEAYWDFAEWFARERKVEYSHSLDYV
                     RQGRNVVVLRTFSKAQGLAAVRVGYGAGPADLMGYFARVRTAFMVSGVAQAAAMASLE
                     DRAHYEKTMRNNAEQSRYLISALREMGCQPVETWSNFIYCEIGENASAVSQRLQQEGI
                     IIRPMTGTWGAPTAIRVTIGTPAQNESFVKAMKKVVASATAAL"
BASE COUNT          858 a         1405 c         1460 g          633 t
ORIGIN      
        1 taagggctag tcaatgtcac tgaaggcaaa gatcgaagca atcatctacg cggccgaaga
       61 gccgatcacg gtcgagcaga tttccgcgct cgtgaaagac atggtgctcg ccgaagaggc
      121 cgagcaggca cgcgctgccg ccgaaatggc cgcctcgggc gaggtcgcgg agcaggccga
      181 agttgccgaa ccctccgcca gcctggagcc ggcggaagcc gccgccggcc agatggacga
      241 accggcggtg gacggcgcag ccgagaccgt tgccggcgag cagtccgtac ccgagccggc
      301 gaacggcacg gagatcgccg cgcccgcgga acagccggac acaatcgagg cgggatcggt
      361 caccgtcgaa ggcccggacg cgacggcgaa caagaagaag ccggcgaaga aagaactgag
      421 cgaggacgcg cgggtgcgcg cacgcgtgcg cagcgtcatc caggaactgg cgtcggactg
      481 cgcgcaagac gaccgaggaa tcgagatccg cgcggtggcc ggcgggttcc gcatgtccac
      541 caagccagaa caccatgacg tggtgcgtgc gttcgccaaa agcctgaagc cgccggtacg
      601 cctgtcgctc gcggcactgg agacactcgc ggtgatcgcc tacaagcagc ccgtgacggt
      661 gcccgagatc agcgaagttc gcggcgtgga atccggcggc gtgatcggaa ctctgctgga
      721 tcgcaagctc atcactacgg ccggccgcaa acaggtcgtc ggacgcccaa tcctgtacaa
      781 gacgagcaag gagttcctgc tgcgcttcgg cctcaacgac atcgccgaac tgccgagcat
      841 ggaggagttc gagaaactcg caagcgacgg acagggcgaa ctgttcacac cggaagcgag
      901 caccgaaccg ccgcatgcgc ccgcgcccgc ggcggaagcg ccggatacct ccgcaccaga
      961 aaccgctccg gacgagccag ttgcagaaga ggcgacgacg gctgcaccgg aagaggtctc
     1021 gaccgaacca acgccccaag cggcggaggc caagacggaa tagcttctaa gcgggttcca
     1081 accacagaga caccgagaca cagagacccg aacgtcacaa ctcggaaggc gttcgggccg
     1141 gacgttcatt ggccgcctcc ggaagctcga ttggcagctt ccgaagatcg caaccgcggg
     1201 agcgccggac caacgaccaa cgaccgacca ccaacgacca attcgtaact gtctctccgc
     1261 ccctgtatca tctccttatg gccgctgaaa gattgcagaa agtgcttgcc gccgcaggta
     1321 tcgcctcgcg ccgcaaagcc gaagaactca ttacagaagg tcgggtgtca atcaacggcc
     1381 acgtggtaac ggaaatgggg acgaaagccg acccgcgcat ggacgagatc cgcgtggatg
     1441 gtcagctgat cggccgcccg gagcgccacg tctatttcat ggtttacaag cccaaggggc
     1501 acgtcaccac ggtcgccgac cctgagggcc gtcccacggt gatgcacctg atggaaggca
     1561 tccgcgagcg ggtcttccct gtcggccgcc tcgactacat gagcgaaggc ctgttgctca
     1621 tgaccaacga cggcgaattg atggcgcaac tgacggctgc caagtcgcac gtgccgaaga
     1681 cgtaccaggt caaggtgagc ggccacccca cggaagagca gatcgacaag ctgcgcgcgg
     1741 gcgtgtggct gccgcccgag ccgggcatga ccggcaagcg cggcaagaag cagcccgggg
     1801 tcgagcgccg ctcgttcggc gtgaagacgc agccggcgca gatcgaactc gtgcgcgacc
     1861 aggagaaccc ctggtacgaa gtgacgctga tcgagggacg caaccgccag atccgtcgca
     1921 tgttcgaaga gatcggattt cacatcgaga agatcaagcg tgtgcgctac ggaaccctgg
     1981 aactcaatgt cgagccaggc cagtggcgcc agttgacctt cagtgagatc aacgaactga
     2041 agcgctcgct gaagaagccg ttcaggatcg ccccgccgcg gccgcgccgg gacacagagg
     2101 aaccggaaag cgctccaggg ccgcgtcgcg aaccgcgcgc ccccaggagc gatgcacgga
     2161 cggcacgggg tccggagcgc ccgcagcgct cgcgcagcga acgcccggga tacgcggagc
     2221 gtccggcccg cgaaggtttc gcagcgcgtc cgcccaggag agaagaaggc ggcgagtcgc
     2281 gcccaccccg ccgtgtaagc ggcggtttcg gggaccgtcc acaacgccgc gaaggcggat
     2341 cgttccaggg gcgtccaccg cgccgggagg gggaaggctt tcagtcacgg ccaccgcgtc
     2401 gagaaagcgg gagcttcccg cgccgcgacc gtgagagctt tgagacccgt ccgcctcgcc
     2461 gggcatccgg tggtttcgac gagcgtccac gccgtgaaag gccagcatcc ggcgaggagc
     2521 gtccacgtcg cgagcgtcct gcttttggcc aggaccgccc gcgccgggag cgtccagcat
     2581 tcggccagga tcgtccccgc cgcgagcgtc cagcttttgg cgaggatcgc ccacgtcgcg
     2641 agcgtccgca gtttggtgaa ggacgtccgc gtcgcgaagg tggcgggttc gggcagcagc
     2701 gcgaaggtgg tactgcccgc gagcgcgggt tcgcagaccg tggcgcgcgc ggcgagcgcc
     2761 ccacgcgcaa cgcaggtccg cgtggcgagc gtccaaccgg tccacgagga gagcggccct
     2821 cagggccccg tggcggccgt cctgcgggtc ctggcggcga gcgtccgtcg ggtgcgcgtg
     2881 gtccgcgccg cgaaggcggc gagggcggcg gcgggcgcgg gttccgccgc gaaggccctc
     2941 cgcgcggcga tcgcccgcag aatcgccgtg gtccaggtag tcgcagttcg ggtcccagga
     3001 atcgtcgcga ctaatttgcg cataggattc ggtgactatg tcgaaattct ctgagtttgt
     3061 accggagcgg attcgtcagc ttccgccgta cgtcccgggc aagcccatca agcaggcgga
     3121 ggccgagtcc ggggttacgg ccatcaagct cgcttcgaac gaaaacccgt ttggtccctc
     3181 gcctctcgcg ttacacgcga tggatgacgc tgcacggtcg gtgcaccttt accccgacaa
     3241 cgacgccacg gagttgaaaa accgcctggc cgcccatcac ggggtcggcg cggagcaagt
     3301 gctcgtcacg ggcggctcca cgccgctgct ggatatcatc gcccgcacgg tgctggctcc
     3361 ggggttgaac gcaatcacca gcgagcgctc gttcatcgtc tatcccatcg tgacgcgctc
     3421 ggctggcgcg gagttccgaa aggtgccgat gaagaacgac accttcgacc tcgacgccat
     3481 cgccgcggcc gtggataaga acacgcggct gatcttcctt gcgaacccca acaacccgac
     3541 ggggaccatc gtcacggccg ccgagcttga tcgcttcctc gacaagcttc cggaccacgt
     3601 gctggtcgca ctcgatgagg cgtactggga ttttgccgag tggttcgccc gcgagcgcaa
     3661 ggtcgagtac tcgcactcgc tcgattacgt gaggcaggga cgcaacgtcg ttgtcctgcg
     3721 gaccttcagc aaggcgcagg gactggccgc cgtccgcgtg gggtatggcg ccggcccagc
     3781 cgacctgatg ggatacttcg cgcgggtgcg cacggctttc atggtgtcgg gcgtcgcaca
     3841 agccgccgcc atggcgtcgc tcgaagatcg tgcgcattac gagaagacga tgcgcaacaa
     3901 cgccgagcaa tcgaggtatc tgatttcggc gctgcgagag atgggctgcc agccggtgga
     3961 aacgtggtcg aacttcatct actgcgagat cggcgagaac gcgtcggcgg tgagccagcg
     4021 gcttcagcag gaaggcatca tcatccgccc gatgaccggc acttggggag cgccgaccgc
     4081 gatccgcgta accatcggga cgcccgcgca aaacgaatcg ttcgtcaagg cgatgaagaa
     4141 ggtcgtggcg agcgcaacgg cggcgctgtg aagtctctgt cttgatcgcc cagcactgac
     4201 gtgctgggct aattcatttg gaatcattcg tggcgtccct gtgggactcg tcgcggtacg
     4261 atctgcattc ccagcactga cgtgctgggc tatatctgtg gcgcgcctgt ggcgctcgtc
     4321 tgcagttggc gcacttcaca cttcacactt cgcact
//
LOCUS       DASPLT010000006         1416 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_61467, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000006 DASPLT010000000
VERSION     DASPLT010000006.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1416)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1416)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1416
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_61467"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(147..308)
                     /locus_tag="VEA63_00070"
     CDS             complement(147..308)
                     /locus_tag="VEA63_00070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD82399.1"
                     /translation="MRGRGRRAGRVQFAELSLPAKPAWSFEVALPNGCVVRAAAASGV
                     AELLGLIRS"
     gene            complement(666..1103)
                     /locus_tag="VEA63_00075"
     CDS             complement(666..1103)
                     /locus_tag="VEA63_00075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD82400.1"
                     /translation="MDLAAKEWKPLIEKNDPDAEFRFGWLLWTNVLGPNRELEAIHLF
                     RKAAEQGQPKALLILGDLYYQSPNNPTWQVKNAPFAKDVEKALGFYLRAQKTAYYPPE
                     KKAVEYVLPKIKAEIQPQRVRAAEIEADAWKPVIVGREPRRLL"
BASE COUNT          271 a          490 c          361 g          294 t
ORIGIN      
        1 ggcgaacacg aacaccgcac cgtgccgcgg atcctcgcgc agtttttcca cggcgagcgt
       61 ccacagcccg ttaaactgtt tgcgcatgtc caccggctcg accgccaggt agaccttcac
      121 gccggcggga aacgcgagca tcggcgtcag ctccggatca aaccgagcaa ctcggccacg
      181 ccgctggccg ccgccgcgcg caccacgcag ccattcggca gcgccacctc gaacgaccac
      241 gccggcttcg ccggcagcga caactcggca aactgcacgc gacccgcccg ccggccgcga
      301 ccgcgcaacc agacccactt ggccagcgtg ttcgcgttga tcccttcgcg ccgcgcaaag
      361 gcgcgtgcgt catcccgctc gcgtcgaact cggccagcag ttccccgcgc cgttcgcgag
      421 cggcgatgcg ccgccccttc gtgtcccgct tctcccgcgc cgccaccact tcggtcgtga
      481 tcgtcgtcat cacgccagat tacccgatcc agtttcagcc ggctatgagg tcacagaccg
      541 gacgcttacg cgatctccaa ggtccagtcc gattgtatag gttttcgatt tcatcaagcg
      601 tagtctgctg ctttcgcgca gctcggctac gccttctcat tcctacgggt tcggctcttc
      661 tttcctcaaa gtagccgcct tggctctcgg ccaacaatca ctggcttcca agcatcagct
      721 tcgatctccg ctgcacggac tctctgcggc tggatctctg ccttgatctt gggaagcaca
      781 tactcgacgg ccttcttctc gggcggatag taagcggtct tctgtgctct gagatagaac
      841 cccagcgcct tctcaacatc cttcgcaaaa ggagcattct taacctgcca cgtcggattg
      901 ttcggacttt gataataaag gtcgcccaaa atcaacagcg ctttcggctg accttgctcc
      961 gccgctttcc ggaataggtg tatcgcttct aactctcgat tcggaccaag aacattcgtc
     1021 cataacaacc atccaaaccg aaattcggca tcagggtcgt ttttctcgat aagcggcttc
     1081 cactctttcg ccgcgagatc cattcgacct cgatcatgcc aacgaagccc cagggtatac
     1141 ggatcgctgg gatcgtaata cgtcccgcag ccggccaagc cgagaactgc acacatcgac
     1201 aacattcgca tcacggttct catggttcgt ttcgcccaac gctaaaggtg agccgcgcga
     1261 tgagcggcgt gagcaaacaa gagcggcgta atcgcgttgg ctctgacgcc tggttcggcc
     1321 gggccgcatc cgccgacgga ctcttctgat tcgctgcggt gccaatcttc acgctgcgaa
     1381 aactggagac aactttttcg ccggtcgatc ctcgaa
//
LOCUS       DASPLT010000007         3172 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_204876, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000007 DASPLT010000000
VERSION     DASPLT010000007.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 3172)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3172)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3172
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_204876"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(11..613)
                     /locus_tag="VEA63_00080"
     CDS             complement(11..613)
                     /locus_tag="VEA63_00080"
                     /inference="COORDINATES: protein motif:HMM:NF012720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HYD82401.1"
                     /translation="MKTPHTLDVSELPDYAFGHRSPMWWATMGIVLIEGTMFVALLGA
                     YFYLRSRAPGWPLNVAPPDLLFGTLNTLILLASCVPNHLYKKAAEKEDLRGVRIWLVV
                     SIVFALAFVVVRAMEFQTLNCRWSDNAYASIVWTLLGFHTAHLVTDLLDTGVLTMLMF
                     TGPLKGKRFVDVAENGMYWYFVVWAWVPIYVTLYLVPRWS"
     gene            complement(610..2508)
                     /gene="ctaD"
                     /locus_tag="VEA63_00085"
     CDS             complement(610..2508)
                     /gene="ctaD"
                     /locus_tag="VEA63_00085"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR02891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit I"
                     /protein_id="HYD82402.1"
                     /translation="MKERESMADAALRPADRLQIDYLEATWSRPSGLIGWLSTTDHKE
                     IGRRFVATAFGFFVAAGILAALMRLQLSRPEAGLLNPDLYNQVFSMHGTTMMFLFAVP
                     VMQAMGIYLVPLMIGTRNLAFPRLTAFGYYVYLFGGLMLYGAFFLNTGVEAGWFSYVP
                     LAGPDFSPGKRTDFYAQMITFTELSAMVEAVNLIATIFKMRAPGMTLNRMPLFVWAIL
                     VTSFMVVFAMPTVATASTMLILDRLVSTQFFNHVEGGDALLWQHLFWFFGHPEVYIIF
                     LPATGMVSAIIVPFMRRAIVGYLPVVLALIATAFAAFGLWVHHMFATGMPKLGQSFFT
                     AASMLIALPSGTQIFCWIATLWLGRPWLKTPLLFVLGFVFTFVMGGLTGVMLASVPLD
                     LQVHDTYFVVAHFHYVLIGGAVFPLFGAFYYWFPKAYGRVLSERIGRWNFALMFAGFH
                     LTFFPMHVLGLEGMPRRVYSYLPDSGWASLNLLASVGAALMATSVALFMANVIRALRR
                     PADAAANPWDAEGLEWATTSPPAVYNFADLPVVRGRYALWEPGEETVVTGLRRDKRLT
                     LVTHVLDAQPDHKMQLPEPSIWPFWSAVAVSALFIGSIFTPWAVVWGSLPVFVTMVCW
                     FWPKRSEE"
     gene            complement(2579..>3172)
                     /locus_tag="VEA63_00090"
     CDS             complement(2579..>3172)
                     /locus_tag="VEA63_00090"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HYD82403.1"
                     /translation="QANEVVETANEIHVPVGRPVHLQLRSADVIHSFWVPRLHGKRDL
                     IPGRDTSLWIRADREGEYRGVCAEFCGHQHAHMQFVVVAQPAEEFEAWLAQQRTAARE
                     PTTERQKRGREVFFSATCVMCHTVRGTPAGSRVGPDLTHFGSRRTLAAAALPNERAEL
                     AKWVLDPQSVKPGVRMPPHAFETEDLEALLDYLESLE"
BASE COUNT          717 a         1171 c          800 g          484 t
ORIGIN      
        1 ctcatcggtc tcagctccag cgcggcacga gataaagcgt cacgtaaatc ggcacccagg
       61 cccacacgac gaaataccaa tacatgccat tctccgccac atcgacgaac ctctttcctt
      121 tcagcggccc cgtgaacatc agcatagtca acacgcccgt atccaaaaga tcggtcacca
      181 agtgcgcggt gtgaaagccg agcagcgtcc acacgatcga tgcatacgca ttatccgacc
      241 agcggcagtt cagcgtctga aactccatcg cccgcaccac cacaaacgcc agcgcgaaca
      301 cgatcgatac cacgagccag atccgcacgc cgcgcaggtc ctccttctcc gccgctttct
      361 tgtagagatg attcggcacg caactcgcca gcaggatcag cgtattgagc gtgccgaaca
      421 acaaatccgg cggcgccacg ttcagcggcc agcccggcgc ccggctgcgc aaataaaaat
      481 acgcgccgag caacgccacg aacatcgtgc cttcgatcaa cacaatcccc attgtcgccc
      541 accacatcgg gctgcgatgg ccaaacgcgt agtcgggcaa ttccgagaca tcgagcgtgt
      601 gcggtgtttt cattcttcgc tcctcttcgg ccagaaccaa cagaccatcg tcacgaacac
      661 cggcaacgat ccccacacca ccgcccacgg cgtgaagatc gacccaatga aaagcgccga
      721 caccgccacc gccgaccaaa acggccagat cgacggctcc ggcagttgca tcttgtggtc
      781 cggttgcgcg tcgagcacgt gcgtcaccaa cgtcagtcgc ttgtcgcgcc gcagtccggt
      841 caccacggtt tcctcgcccg gctcccacaa cgcataacgc ccgcgcacca ccggcagatc
      901 cgcgaaatta taaaccgccg gcggcgaggt cgtcgcccac tcgaggcctt ccgcgtccca
      961 cggattcgcc gccgcatccg ccggccggcg caacgcccga atcacgttcg ccataaacag
     1021 cgccacgctc gtcgccatca gcgcggcgcc gacgctggcg agcaggttca aactcgccca
     1081 gccgctgtcg ggcaaatatg aatacacccg ccgcggcatg ccttcgaggc caaggacgtg
     1141 catcggaaaa aacgtcaggt gaaagcccgc gaacatcagc gcaaaattcc accggccgat
     1201 ccgctcgctc agcacccggc catacgcctt cggaaaccag tagtaaaacg cgccgaacag
     1261 cggaaacacc gcgccgccga tcagcacgta atggaagtgc gccaccacga aataggtgtc
     1321 gtgcacctgc agatcgagcg gcaccgacgc cagcatcacc cctgtcagcc cgcccatgac
     1381 aaacgtgaac acaaaaccga gcacgaacag cagcggcgtt ttcaaccagg gtcgccccag
     1441 ccacagcgtc gcaatccagc agaagatctg cgtcccgctc gggagcgcga tcaacatgct
     1501 cgccgccgtg aaaaaactct gaccgagctt cggcatgccc gtcgcgaaca tgtggtgcac
     1561 ccacaatccg aacgccgcaa acgccgtcgc aatcaacgcc agcaccaccg gcaaataacc
     1621 gacgatcgcc cgccgcatga acggcacgat gatcgccgac accattcccg tcgccggcag
     1681 gaaaatgatg tagacctccg gatgcccgaa gaaccagaag aggtgctgcc acagcaacgc
     1741 atcgccgcct tcgacgtggt tgaagaactg cgtgctcacc agccgatcga gaatcagcat
     1801 cgtgctcgcc gtcgccaccg tcggcatcgc aaacaccacc atgaacgagg tcaccaggat
     1861 cgcccaaaca aacagcggca tccggttcaa cgtcatgccc ggcgcgcgca tcttgaaaat
     1921 cgtcgcgatc aagttcaccg cctcgaccat cgccgacagc tccgtgaacg tgatcatctg
     1981 cgcataaaaa tccgtgcgct ttcccggcga gaagtccggt cccgccagcg gcacgtaact
     2041 gaaccagccg gcctccacgc ccgtgttcag gaaaaacgcc ccgtaaagca tcaatccacc
     2101 gaacaggtaa acgtagtagc caaacgccgt gagccgcgga aacgccaggt tccgcgtccc
     2161 gatcatcagc ggcaccagat aaattcccat cgcctgcatc accggcacgg cgaacagaaa
     2221 catcatcgtc gtgccgtgca tcgagaacac ttggttgtag agatcgggat tgagcagccc
     2281 cgcctccggt cggctcaact gcaagcgcat caacgccgcg agaatccccg ccgccacaaa
     2341 aaacccgaac gccgtcgcca cgaaacgccg cccgatctcc ttgtgatcgg ttgtcgacag
     2401 ccacccgatc aagcccgacg gccgcgacca cgtcgcctcg agataatcga tctgcaaccg
     2461 atccgccggt cgcaacgccg catccgccat cgattcacgc tctttcatcg cgcccgcctt
     2521 ccgtagcgaa aaacgtaagt ttttcgtccc ctcaccgcgt cgttaaatag cgcgcgtttc
     2581 attcgagact ctccagataa tccagcaacg cctccaggtc ctccgtctcg aacgcatgcg
     2641 gaggcattcg cacgccgggc ttcacgcttt gcggatcgag cacccacttc gcgagctccg
     2701 cccgctcgtt cggcaacgcc gccgccgcca gcgtgcgccg gcttccgaaa tgcgtgaggt
     2761 ccggccccac ccgactcccc gccggcgtgc cgcgcacggt gtgacacatc acgcacgtcg
     2821 ccgaaaaaaa cacctcgcgc ccgcgcttct gccgctccgt cgtcggctcc cgagccgccg
     2881 tgcgctgctg tgccagccac gcctcgaatt cctccgctgg ctgcgccacc accacgaatt
     2941 gcatgtgcgc gtgctggtgg ccgcaaaact ccgcgcacac gccgcgatac tcaccctccc
     3001 ggtccgcgcg tatccacaaa ctggtgtcac gccccgggat gagatcgcgc ttcccgtgca
     3061 accgcggcac ccaaaaacta tggattacat ccgccgatcg gagttgcagg tgcaccggcc
     3121 ggccgaccgg cacatggatc tcgttcgccg tttccaccac ttcgttcgcc tg
//
LOCUS       DASPLT010000008         1176 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_47, whole genome
            shotgun sequence.
ACCESSION   DASPLT010000008 DASPLT010000000
VERSION     DASPLT010000008.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1176)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1176)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1176
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_47"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(153..578)
                     /gene="fliS"
                     /locus_tag="VEA63_00095"
     CDS             complement(153..578)
                     /gene="fliS"
                     /locus_tag="VEA63_00095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HYD82404.1"
                     /translation="MVANGYVKTYRSNAVLTASPGQLVLMLYDGALKAMAIAIEAFER
                     PEDDTGRIQVINHQLQKAQNIIAELQNGLNLEAGGDFAQTLFRLYDYHNRRLFEANLK
                     KDVAIIHEVDNLVRSLRDAWAEMLTKQEEPHTESLRGVA"
     gene            complement(588..872)
                     /locus_tag="VEA63_00100"
     CDS             complement(588..872)
                     /locus_tag="VEA63_00100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007363274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD82405.1"
                     /translation="MINSTTSTDRTPHANTVAVAGKPAQRAATQRPDQLSTDSDAFLR
                     AELQRQPEIRPEVVERARALAADSNYPSLDTLKKVGAIILNTPDLSEDQS"
     gene            complement(966..>1176)
                     /locus_tag="VEA63_00105"
     CDS             complement(966..>1176)
                     /locus_tag="VEA63_00105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD82406.1"
                     /translation="FSAADPAVENSARHHFIDSDRGRTPADLTLAVLQSPTRFATYVA
                     AATFATLPPVYTAPENTDVSVADPR"
BASE COUNT          211 a          320 c          394 g          251 t
ORIGIN      
        1 catttcgaaa aatttttcgc cgctgcttcg cagtccgtcc tggaataccc aaagtttgaa
       61 tacggcgggt tcatgcgttg cggctcggag agtgttgcgg gcaggacgtc agtaaggccg
      121 cttcgtcgag tgcgcggtgg aggaaatctc cttcacgcca cgccgcgcag gctctcggtg
      181 tgcggctctt cctgtttcgt cagcatctcg gcccatgcat cgcggagcga gcggaccaga
      241 ttgtcgactt cgtgaatgat cgccacgtcc ttcttgaggt tggcttcgaa cagccggcgg
      301 ttgtgatagt cgtagaggcg aaaaagcgtc tgcgcgaaat ctccacccgc ttcgaggttc
      361 aatccatttt gcagctcggc gatgatgttc tgcgcctttt ggagctggtg gttgatgact
      421 tggatccggc cggtgtcgtc ctcgggacgc tcgaaggcct cgatcgcaat ggccatcgcc
      481 ttgagcgcgc cgtcgtagag catcaacacg agctggccgg gactggccgt gaggaccgcg
      541 ttggagcggt aagtcttaac gtagccgttg gcgaccatgg ccggggctta cgactgatct
      601 tcgctgaggt caggcgtgtt cagaatgatc gcgccgacct ttttcaaagt gtcgagagaa
      661 ggataattcg aatcggccgc gagggcgcgg gcacgttcca ccacttccgg gcggatttcc
      721 ggctgacgct gaagctcggc gcgcaaaaac gcgtcgctgt ccgtcgaaag ctgatcgggg
      781 cgctgcgtcg ccgcgcgttg cgccggcttg ccagccacgg ccacggtgtt ggcgtgcgga
      841 gtcctgtcgg tggaggtcgt agagttgatc attgggtttc gtggaacgaa acggtcagcc
      901 tctgaggtca gttacaggga acgccgttgg ttgaaggctc aatatcggtc tgaaagcaga
      961 atactttagc gcgggtccgc aactgaaacg tcggtgtttt cgggtgcggt gtaaaccggt
     1021 ggcaacgtcg caaacgtcgc cgccgccacg taggtcgcga agcgcgtcgg cgactgcaac
     1081 acggcgagcg tgagatcggc cggagtccgg ccgcgatccg agtcgataaa gtggtgccgc
     1141 gcgctgttct caacggcggg atcggccgcg gaaaag
//
LOCUS       DASPLT010000009         1093 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_69, whole genome
            shotgun sequence.
ACCESSION   DASPLT010000009 DASPLT010000000
VERSION     DASPLT010000009.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1093)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1093)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1093
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_69"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..325)
                     /locus_tag="VEA63_00110"
     CDS             complement(<1..325)
                     /locus_tag="VEA63_00110"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HYD82407.1"
                     /translation="MATPPLRVFLVEDSVVIRQRISEALAQLGGIEIVGSADAEDEAV
                     RALREIHCDVVVLDLQLRQGNGLGVLKTMRAAPGAARPLYIVFTNYDFPHYRAKSAAL
                     GADHFF"
     gene            complement(354..>1093)
                     /locus_tag="VEA63_00115"
     CDS             complement(354..>1093)
                     /locus_tag="VEA63_00115"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HYD82408.1"
                     /translation="PMSRSGWEAVMLEAQSNDMGVYEQTSQPGRTPPTQIRILIADDH
                     AIVRAGLKQFIADEPDMAVTGEAETGAQTVELVRNGEWDVVLLDISMPDRSGVDTLKS
                     LRHLRPELPVLILSGFPEGQYAVNLLRAGASGYLNKESAPSELVRAIRTVVSGRRYVS
                     ATLAEILAHDVSTPASDRPVHTALSEREFQIFCKLAAGQAVSKIAEELFLSVKTVSTY
                     RTRILEKMNMKTNADLTYYAIKNHLIQ"
BASE COUNT          188 a          349 c          345 g          211 t
ORIGIN      
        1 cgaagaagtg atccgcgccg agcgccgcgc tcttggcgcg gtaatgcgga aaatcgtaat
       61 tggtgaagac gatatagagg gggcgggcgg caccgggcgc tgcgcgcata gtcttcaaca
      121 ccccgaggcc gtttccctgg cgcaactgca gatcgagcac cacgacgtcg caatgaattt
      181 cgcgcagtgc gcgcaccgct tcgtcctcgg cgtctgccga gccgacgatc tcgatgccgc
      241 cgagttgggc gagcgcttcg gagatgcgct gccggatgac aacgctatcc tccaccagga
      301 aaacgcgcag gggcggcgtg gccatcgtct cctttccgac cgcggcgcgg cgactactgg
      361 atcaagtggt tcttgatcgc gtagtaggtg aggtccgcgt tcgtcttcat gttcatcttc
      421 tccaggattc gcgtgcggta ggtgctgacg gtcttcacgg agagaaacag ctcctcggca
      481 atcttggaaa ccgcctggcc cgcggcgagc ttgcagaaaa tctggaattc gcgctcggaa
      541 agcgcggtat gcaccggacg gtcgctcgcc ggcgtactga cgtcgtgcgc gaggatctcc
      601 gcgagcgtcg cgctgacgta gcggcgtccg gatacgacgg tgcggatcgc gcgcaccagt
      661 tcggacggcg cgctttcctt gttgagataa ccgctcgccc ccgcgcgcaa caggttgacg
      721 gcgtattgcc cctccggaaa cccggacagg atcaataccg gcagttcggg acgcagatgc
      781 ctgaggctct tcagcgtatc gacgccactc cgatcgggca tggagatgtc gagcagcacc
      841 acgtcccatt cgccgtttcg cacaagttcg accgtctgcg cgccggtctc cgcctctccc
      901 gtcaccgcca tgtcgggttc atcggcgatg aattgcttga gccctgctct cacaatggcg
      961 tgatcgtccg cgatcaggat gcggatctgg gtaggcggcg tgcgtcccgg ctgcgacgtc
     1021 tgttcgtaca cacccatgtc gttgctctga gcctcaagca tgaccgcctc ccagcccgat
     1081 cggctcatcg gcg
//
LOCUS       DASPLT010000010         2833 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_245811, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000010 DASPLT010000000
VERSION     DASPLT010000010.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 2833)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2833)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2833
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_245811"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..730
                     /locus_tag="VEA63_00120"
     CDS             <1..730
                     /locus_tag="VEA63_00120"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HYD82409.1"
                     /translation="AQFGYYEGQVQWLENLGEWKFAERPLLDLPGAIHAPIVDYNRDG
                     KPDIVALVSQDWEEVWGFQNRGGGDFERRVLQGSTNRDYGSSGLCIADVDRDGHSDIV
                     YTNGDGFDYATPGSRPWHGVQWLRNDGQGNFRFRRIGDFAGAYSPVVVDLDDDGDRDV
                     VAVSAFNDWAKPEVVSLMCFENDGRETFTPRVLAHAPTHLVVAKAADMDGDGRVELIT
                     GSFAFYPPLDRAGRVTLWEGARTR"
     gene            736..1545
                     /locus_tag="VEA63_00125"
     CDS             736..1545
                     /locus_tag="VEA63_00125"
                     /inference="COORDINATES: protein motif:HMM:NF018178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HYD82410.1"
                     /translation="MKQKNRKWSSVFVVALGVIAGALASVSARSAEAKSDGWIPLFNG
                     KDLTGWTPKIAKHAIGVNYLDTFRVEDGIIKVSYDKYRIFDQQFGHLYTNSPYSHYVI
                     RLEYLITGETMKDAPSWAGFNSGVMVHSQSPLSMTVEQLWPVSLEGQFLVKGTTAGTQ
                     TGNVCTPGTDVEVNGKLTKAHIVDSTGPMPEVDTWVRFEAEVRGHDEIVYRVNGVEVL
                     RYTRPRLDETDPDAQRLLAAGADPRVSFGHIALQAEGHPVWFRNIELRPLK"
     gene            1629..2519
                     /locus_tag="VEA63_00130"
     CDS             1629..2519
                     /locus_tag="VEA63_00130"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HYD82411.1"
                     /translation="MKITALVLCIVFLWGGMGSAAALDSVVVPLWAGVAPGSEGKTGE
                     ENVRLSEGGEHIVSNIHRPSLTVYLPSGGAATKLVAGVIVIPGGGHRELWMDHEGYNI
                     AQRLAARGIAAFVLKYRLAREEGSTYRVDRESLRDVQRALRLVRHRASEWRVDPARLG
                     VMGFSAGGELAALAAMHVEPERGETAAGDAIDRESARPAFQALIYPGKSDAIEPGEKA
                     PPAFLVCGAQDRPDISEGLARAYLRFKLARVPAELHIYAHAGHGFGLRATNRSALAGW
                     LERFGEWLVDGGFVEGASAE"
     gene            2536..>2833
                     /locus_tag="VEA63_00135"
     CDS             2536..>2833
                     /locus_tag="VEA63_00135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD82412.1"
                     /translation="MTRHDARKKQAATEGCRYVVFALACCVAVAAAADLPPGIEAFDF
                     APRAERTGPTLFSRIDASVSGVHAPSIYDDPAMWTERFERFAYGSMGTGVAIGDY"
BASE COUNT          488 a          760 c         1040 g          545 t
ORIGIN      
        1 ggcgcagttc gggtattacg aagggcaggt gcagtggctg gagaatctcg gggaatggaa
       61 gtttgccgag cgcccgttgc tggatttgcc gggtgcgatt cacgcgccga ttgtcgacta
      121 caatcgcgac ggaaagcccg acatcgtcgc gctggtttcg caggattggg aagaggtgtg
      181 gggtttccag aaccgcggcg gcggcgactt cgagcgacgc gtgctgcagg gatcgacgaa
      241 tcgcgactac ggcagcagcg ggctttgcat cgcggacgtg gatcgcgacg gacacagcga
      301 catcgtttac acgaatggcg acggcttcga ctatgcgacg ccgggctcgc gtccctggca
      361 cggcgtgcaa tggctgcgga atgacgggca gggaaatttt cgttttcggc gcatcggcga
      421 ttttgcaggc gcttacagtc cggtcgtcgt cgatctcgat gacgatggcg accgcgacgt
      481 ggtggcggtc agcgcgttca acgactgggc gaagcccgag gtggtgtcgt tgatgtgctt
      541 cgagaacgat ggacgcgaga cgttcacgcc gcgggtgctc gcgcatgcac cgacgcacct
      601 cgtggtggcg aaagcggcgg atatggatgg cgacgggcgc gtggagttga tcaccggaag
      661 ctttgcgttt tatccgccgc tcgatcgagc cgggcgggtc acgctctggg agggggcgcg
      721 aacgcgttga attttatgaa acaaaaaaat cggaaatgga gttcggtgtt cgtcgtcgcg
      781 cttggcgtta tcgccggtgc gctcgcgtcg gtttcggctc gaagcgcgga ggcgaaaagc
      841 gacggttgga taccgttgtt caacggcaag gatctcacgg gttggacacc gaagatcgcg
      901 aagcacgcga tcggtgttaa ctatctggat acgtttcgcg tcgaggacgg aatcatcaag
      961 gtgtcctatg acaagtatcg gattttcgat cagcagttcg gtcatcttta cacgaacagc
     1021 ccgtattcgc actacgtgat ccgactggaa tacctgatca cgggcgagac gatgaaggac
     1081 gcgccgtcgt gggcggggtt caacagcggc gtgatggtgc actcccagtc gccgttgagc
     1141 atgacggtcg agcagttgtg gccggtgagt ttggaagggc agttcctcgt gaaaggcacg
     1201 acggcgggaa cgcagacggg caatgtgtgc acgccgggca cggacgtcga agtgaacggg
     1261 aagctcacga aagcacacat cgtcgattcg accgggccga tgccggaggt cgacacgtgg
     1321 gtgcgcttcg aggcggaagt gcgcgggcac gacgaaatcg tttatcgggt gaacggcgtg
     1381 gaagtgttgc gctacacgcg tccgcggctc gatgagaccg atcccgatgc gcaacgcctg
     1441 ctcgctgcgg gcgccgatcc gcgcgtgagt tttggccaca tcgcgctgca agccgagggt
     1501 caccccgtgt ggttccgcaa catcgagcta cgtccgctga agtagggcgg ggttcatgcc
     1561 gcttccgcac cgataaccga cagagaaggc gggctggagc ccgccctact gttactgcca
     1621 ttcatgctat gaaaataacc gcgctcgttt tgtgtatcgt gtttctgtgg ggaggaatgg
     1681 ggtcggccgc ggctctcgat tccgtcgtcg tgccgttgtg ggctggcgtc gcgcccggct
     1741 cggaaggaaa aaccggcgag gagaacgtgc gcctctccga gggcggagag cacatcgtgt
     1801 cgaacattca ccgaccgtcg ctcacggttt atctgccgag tggcggcgct gcgacgaagc
     1861 tagtcgccgg cgtgattgtg attccgggcg gcgggcatcg cgagctgtgg atggatcacg
     1921 aaggttacaa cattgcgcaa cggctcgcgg cgcgcggcat tgcggcgttc gtgctgaaat
     1981 accgcctcgc gcgcgaggag ggctcgacgt atcgggtgga tcgcgagtcg ttgcgcgacg
     2041 tgcagcgggc gctgcggctg gtgcgtcatc gcgcgagcga gtggcgcgtc gatcccgcgc
     2101 ggctgggcgt gatgggtttt tccgctggcg gcgaactcgc ggcgctggcg gcgatgcacg
     2161 ttgagccgga gcgcggtgaa acggcggcgg gcgatgcgat cgaccgcgaa agcgcacggc
     2221 cggcgtttca ggcgctgatt tatccgggaa aatcggacgc catcgagccc ggcgagaagg
     2281 ccccgccggc gttcctcgtt tgtggcgcgc aggatcgtcc cgacatttcg gaagggctgg
     2341 cgcgcgctta tctgcggttc aagctggctc gtgtgccggc ggagctgcac atctatgcgc
     2401 atgcgggcca cggtttcgga cttcgcgcga cgaatcgttc tgctttggcg ggctggctgg
     2461 aacggtttgg cgaatggctg gtggatggcg ggtttgtcga gggcgcgagc gcggagtaga
     2521 ggcggaatgg aaaacgtgac gcggcacgat gcgcggaaga agcaggcagc cacggagggc
     2581 tgccgctacg tggtgttcgc gctcgcgtgt tgcgtggcgg ttgctgccgc cgcggacctg
     2641 ccgccgggca tcgaggcgtt tgatttcgcg ccgcgcgcgg agcgcacggg gccgaccttg
     2701 ttttcgcgga tcgatgcatc ggtgtcgggt gttcacgcgc cgagcattta tgacgatccg
     2761 gcgatgtgga cggagcgctt cgagcgtttc gcctatggct cgatgggcac gggtgtcgcg
     2821 atcggcgact acg
//
LOCUS       DASPLT010000011         1463 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_225379, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000011 DASPLT010000000
VERSION     DASPLT010000011.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1463)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1463)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1463
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_225379"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..180
                     /locus_tag="VEA63_00140"
     CDS             <1..180
                     /locus_tag="VEA63_00140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD82413.1"
                     /translation="RAIDEVPDVPDIRKTVDEALEDKPKRPPLPPASPNPAPSTATPA
                     AASPAAPAAPPPPIE"
     gene            210..467
                     /locus_tag="VEA63_00145"
     CDS             210..467
                     /locus_tag="VEA63_00145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009511065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HYD82414.1"
                     /translation="MPLPITGELDLHTFRPSDLGELIPAYLAECAARNIREVRIVHGK
                     GTGTLRSSVHALLQRSPLVQSFRLGDEHSGPWGATLVTLRS"
     gene            464..>1463
                     /locus_tag="VEA63_00150"
     CDS             464..>1463
                     /locus_tag="VEA63_00150"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ectonucleotide
                     pyrophosphatase/phosphodiesterase"
                     /protein_id="HYD82415.1"
                     /translation="MTRRAFLHLFAAAFSFCLATALSRASDHASVEPSTPPPLLLISL
                     DAFRWDYCDLHPDDTPHLRALMRSGISARGLVPVFPSNTFANHYTIVTGLYPSHHGII
                     NNNFFDPTFREFFRPFPTKTLQESRWWGGEPVWVTAVKQGRKSATSFWIGSEAEIAGH
                     RPSDWIAFDPRKQPTFDERLDQLFSWLHRPAETRPAFVTFYFEETNSIGHKFGPGSPE
                     LIATIRRLDEQLGRIVARLKSEGIAANIVIVSDHGLTPISTERVILLDDYIAPDSVQL
                     DFDGPVAGLRPHDGEIEKLVAALAPLQHARAYRREELPARFHVTDNPRNPPVWIVPD"
BASE COUNT          254 a          576 c          370 g          263 t
ORIGIN      
        1 cgcgccatcg acgaagtgcc cgacgtgccc gacatccgca aaacggtcga cgaagcgctc
       61 gaggacaaac caaagcgccc tccgcttccg cccgcgtctc cgaaccctgc gccttccacg
      121 gcgaccccgg ccgcggcatc acccgccgca ccggccgctc cgccgccacc gatcgagtga
      181 gcgcctcctt gccgcgcgac gaatccgccg tgcccctgcc gatcaccggc gagctcgatc
      241 tgcacacgtt tcgcccttcc gatctcggcg aactcattcc cgcttacctc gccgaatgcg
      301 ccgcccgcaa tatccgcgag gtgcgcatcg ttcacggcaa gggcaccggc acgctccgca
      361 gctccgttca cgcgctcttg caacgctctc cgctcgtgca atcgtttcgc ctcggcgacg
      421 aacacagtgg cccctggggc gccaccctcg tcactctgcg ctcatgaccc gccgcgcgtt
      481 tctccatctc ttcgccgccg cgttttcatt ctgcctcgcg accgcgctca gccgcgcgag
      541 cgaccacgcc agcgtcgagc cgtccacccc gccgccacta ctgctgatct cgctcgacgc
      601 gtttcggtgg gattactgcg acctgcaccc cgacgacacg cctcatctcc gcgcgctcat
      661 gcgcagcggc atttccgcgc gcggactcgt tcccgtcttt ccgtcgaaca cgttcgccaa
      721 ccactacacg atcgtcaccg gcctctaccc gtcgcaccac ggcatcatca acaacaactt
      781 tttcgatccg accttccgcg aattctttcg tcccttcccg acgaaaacgt tgcaggaatc
      841 gcgttggtgg ggcggcgagc ccgtgtgggt caccgccgtc aagcaaggcc gtaaaagcgc
      901 gacgtcgttt tggatcggct ccgaagccga aatcgccgga caccggccga gcgattggat
      961 cgctttcgat ccgagaaaac agccgacgtt cgatgaacgc ctcgaccaac ttttttcctg
     1021 gctccatcgt ccagcggaaa cgcgccccgc tttcgtcacg ttctatttcg aggaaaccaa
     1081 cagcatcggt cacaaattcg gccccggctc gccggaactc atcgccacga ttcgccgcct
     1141 cgacgaacaa ctcgggcgca tcgtcgcccg gctgaaatct gaaggcatcg ccgccaacat
     1201 cgtcatcgtt tccgatcacg gcctcacgcc gatcagcacc gagcgcgtca tcctcctcga
     1261 cgactacatc gcgcccgatt ccgtgcagct cgacttcgac gggcccgtcg ccggtctgcg
     1321 ccctcacgat ggagagattg aaaaactcgt cgccgcgctc gcgccacttc agcacgcgcg
     1381 cgcatatcgc cgcgaagagc tgcccgcacg ttttcacgtc accgacaatc ctcgcaatcc
     1441 tcccgtatgg atcgtgcccg acg
//
LOCUS       DASPLT010000012         1215 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_269, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000012 DASPLT010000000
VERSION     DASPLT010000012.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1215)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1215)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1215
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_269"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..214)
                     /locus_tag="VEA63_00155"
     CDS             complement(<1..214)
                     /locus_tag="VEA63_00155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007360865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD82416.1"
                     /translation="MIKRAESLHIGRRPGGVGWLMLAPLLLWLTAFVVVPMGILAVYS
                     FCERDELGRVVFSFSLENYARVFSPVY"
     gene            complement(258..>1215)
                     /locus_tag="VEA63_00160"
     CDS             complement(258..>1215)
                     /locus_tag="VEA63_00160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009510031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein"
                     /protein_id="HYD82417.1"
                     /translation="ADELNLFAWSEYVPQDVIDGFTEETGIKVNYETFASGEEMLAKL
                     LAGGASYDLIQPPDYIAEALIKNKLLAPIDAKKVPNLKNVAKEFLGMPHDPTQAFTVP
                     YMIGTVGIVVNTDVVKEPIRGYKDVFQPKYRNRIVALNDNRELVSWVLVTLGYDQNDI
                     SPANLAKVRPVLAEWIKLVKVFDSDSPKTALLSGDVDLGVVWSGEAARLFEEDKKFAY
                     VLPAEGAHPFVDVLAIPTTAEHVDAAHKFLDYILRPDVSKLISDEFPYTNPNLEARKL
                     LTPEQLANPASYPKMEKYDTFRDIGKGAAAIDQLVTDLKSSQ"
BASE COUNT          234 a          397 c          338 g          246 t
ORIGIN      
        1 ggtaaaccgg gctgaacacc cgcgcataat tctccagcga aaagctgaat accactcggc
       61 cgagctcgtc gcgctcgcag aaactataga ccgcgagaat ccccatcggc acaaccacga
      121 acgccgtgag ccacaacagc agcggcgcca gcatcagcca gccgacgcca ccgggccggc
      181 gcccgatatg gagcgattca gcgcgcttga tcaccacgtg agttcaaccg cgcgaagccg
      241 accacgttcg cgctcgttca ctgcgaactc ttgagatccg ttacaagctg gtcgatcgcc
      301 gccgcgcctt tgccgatgtc gcgaaacgtg tcgtatttct ccatcttcgg ataactcgcc
      361 ggattcgcga gctgctccgg cgtcagaagc ttccgcgcct cgaggttggg attcgtgtaa
      421 ggaaactcgt ccgagatcaa tttgcttacg tccggccgca ggatgtaatc gagaaacttg
      481 tgcgcggcgt cgacgtgctc ggccgtcgtc ggaatcgcaa gcacgtcgac aaacggatgc
      541 gcgccctccg ccggaagaac gtaagcgaac ttcttgtcct cttcgaacaa ccgcgccgcc
      601 tcgccgctcc acaccacgcc gagatcgacg tcgccgctca agagcgccgt cttcgggctg
      661 tcggagtcga acaccttcac gagcttgatc cactccgcca gcaccggccg caccttcgcg
      721 aggttcgccg gcgagatgtc gttctggtcg tagccgagcg tgacgagcac ccagctcacc
      781 agctcgcgat tgtcgttcaa cgccacgatg cgattccgat acttcggttg aaacacgtcc
      841 ttgtagccgc gtatcggctc tttcactacg tcggtgttca ccacgatgcc gacggtgccg
      901 atcatgtagg gcacggtaaa cgcctgtgtc ggatcgtgcg gcatgccgag gaactccttc
      961 gccacgttct tcagattcgg cactttcttc gcgtcgatcg gcgcgagcag tttgtttttg
     1021 atcaacgcct cggcgatgta gtccggcggc tggatcagat cgtagctggc gccgccggcg
     1081 agcagcttcg cgagcatctc ttcgccggaa gcgaaggttt cgtagttcac cttgattccc
     1141 gtctcttccg tgaagccgtc gatcacgtct tgcggcacat attcggacca ggcgaacaag
     1201 ttcagttcat cggcc
//
LOCUS       DASPLT010000013         1107 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_205286, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000013 DASPLT010000000
VERSION     DASPLT010000013.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1107)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1107)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1107
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_205286"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..521)
                     /locus_tag="VEA63_00165"
     CDS             complement(<1..521)
                     /locus_tag="VEA63_00165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HYD82418.1"
                     /translation="MSDNTAPAEVSMDNLVALAKRRGFVFQSSEIYGGFNGFFDYGPL
                     GVELKKNIRDCWWDDMVRRRDDIVGIETSIIMHPKVWEASGHVAGFTDPLVDCKASKQ
                     RFRADQLFFSPVIVDGKTVGYVSALESENTAGELQAAAETFKRKKAIQGTLAPVQPRD
                     FTEAKPDEIALIPS"
     gene            930..>1107
                     /locus_tag="VEA63_00170"
     CDS             930..>1107
                     /locus_tag="VEA63_00170"
                     /inference="COORDINATES: protein motif:HMM:NF021082.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2383 domain-containing protein"
                     /protein_id="HYD82419.1"
                     /translation="MNISNDKNISVLNDLIDTCKDGQQGYATAAKDAKDAELARVFSH
                     YAAQRGNYIRELQQR"
BASE COUNT          217 a          293 c          383 g          214 t
ORIGIN      
        1 gaggggatga gcgcgatttc gtcgggtttc gcttcggtga agtcgcgcgg ttggacggga
       61 gcgagcgtgc cttggatggc tttcttacgc ttgaaggttt ccgcggcggc ctggagttcg
      121 ccagcggtgt tttccgattc gagtgcggag acgtagccga cggttttgcc gtcgacgatc
      181 accggcgaga aaaagagttg gtcggcgcgg aagcgttgct tcgaggcttt gcagtcgacg
      241 agcgggtcgg tgaagccggc gacgtggccg gacgcttccc agaccttcgg gtgcatgatg
      301 atggacgtct cgatgccgac gatatcgtcg cggcggcgga ccatgtcgtc ccaccagcag
      361 tcgcgaatgt tcttcttcaa ctcgacgccg agggggccgt agtcgaagaa gccgttgaag
      421 ccgccgtaga tctcggagga ttggaacacg aaaccgcggc gcttcgcgag cgcgacgagg
      481 ttgtccatgg agacttcggc gggagcggtg ttgtcggaca tagcccgtca gcaatggcgg
      541 gcgcggcgcg gcgggtcaag gcgggccgcg gcgctgctgc gccgcgcaag ctccaagcga
      601 gaacaagctc caagcaccaa catccaagct ccagtgaaac ttcaaattcc aagatccaag
      661 aacaaccgct cgtgtgattg attgagtttt gaggttttgt tggagcttca ctggagcttg
      721 gtgcgggccg ctgcgcgctc gtgcaacatg cgcgggattt ctggcgatcc ttgcatcgcg
      781 cacatgcgga gcggagggag gttttgtcgg taaagcacgc gggagaagag gttgcgacga
      841 cgatgtgcga tggcacgtgt taggcaaagg gaggcgctcg gttatgacgg ctctctggcc
      901 gtttcgccga cgcgaaccac tcatcagcaa tgaatatttc gaacgataaa aacatcagcg
      961 tgctcaacga cctcatcgac acctgcaagg atggccagca gggttacgcc acggcggcga
     1021 aggacgccaa ggacgctgaa ctggcgcggg tgttttccca ctatgcggcg cagcgcggta
     1081 actacatccg ggagttgcag cagcgcg
//
LOCUS       DASPLT010000014         2833 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_20874, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000014 DASPLT010000000
VERSION     DASPLT010000014.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 2833)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2833)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2833
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_20874"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            229..1107
                     /locus_tag="VEA63_00175"
     CDS             229..1107
                     /locus_tag="VEA63_00175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD82420.1"
                     /translation="MPIPYIVLTAVVVSLIVMQINQRVASPLLSIVDRWLRWLVFAFG
                     GAHLCAYYELIDRPYPVLVALFFILWFLAETFLNWLRIHALSVSPLPLFPRYEVNEDG
                     DEWPIQPRLLKVREWLRAEGFKQIQALKAEIGGGIYLRVSIYQDPEATIRVQVTFIPQ
                     ASGAITVCYSIASLTSDGARYVTDNLYVPFAGFYPEHWFVERAPWRRSLARLLARHRA
                     RLSRTGAALEPFADDPLTDLNSTQQELDRINTDLGFLHQPPQREDLGKITHEGRYRVW
                     KEIWMLNYLGRAMRYE"
     gene            complement(1258..2274)
                     /gene="moaA"
                     /locus_tag="VEA63_00180"
     CDS             complement(1258..2274)
                     /gene="moaA"
                     /locus_tag="VEA63_00180"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HYD82421.1"
                     /translation="MGQPTDQFNRPLRDLRISVTDRCNFRCPYCMPKEVFGPGHAFLR
                     DPQLMSLAEIVRIVRAFRALGMEKVRLTGGEPLLRADVAELVRVLKSELQIRDVALTT
                     NGWLLEKFAPALRAAGLDRVNVSVDSLDDATAGRMNGLGFKVERVLRGIDAAAAAGLA
                     VKINTVVQRGVNDGELLALCEYFRERGHPLRFIEFMDVGNTNHWTPDNVVPAREIVAR
                     IAEKWPLEASGPAYRGEVAARYRYRDGRGEIGLISSVTEPFCRDCHRARLSADGKLYT
                     CLFASLGWDVLGCLRAGADDAELERYLARIWGGRMDRYSDERAEVLAAGEAREKIEMS
                     YIGG"
     gene            2384..>2833
                     /locus_tag="VEA63_00185"
     CDS             2384..>2833
                     /locus_tag="VEA63_00185"
                     /inference="COORDINATES: protein motif:HMM:NF015418.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD82422.1"
                     /translation="MLTPAEADKLIAQNLAPFLREDCPLASAHGRILREHLRADRDLP
                     PFDRVTMDGFALRSATFAAGTRTFRIEGTQAAGMRPLKLSAADEACVEVMTGAVLPEG
                     ADCVVPYEDTTRDGTTLTIAEAISLTPGSAVHRRGSDHPANDVIVPAG"
BASE COUNT          508 a         1066 c          773 g          486 t
ORIGIN      
        1 ggtttcgtgg atactaccgt tgttgagccc ggtggaacgc cgccgacagc ccggaaagat
       61 tcatcgctct ggatcgcgat gttaaagcag atcgccgcca tctcccttgc aaagggcgtc
      121 gggaacacgc ccgccgaata atttcgctcc tcgcaacggc actgtcttcc ctatggcttt
      181 ttccgtcaac cgcgccaaat ttagtccggc tccacgcgcc acctcctcgt gcccattccc
      241 tatatcgtcc tgaccgccgt cgtcgtttcg ttgatcgtca tgcagatcaa ccagcgggtc
      301 gcgtcaccgc tgctctcgat cgtcgaccgc tggctccgct ggctcgtgtt cgcgttcggc
      361 ggcgcgcacc tttgcgcgta ttacgagcta atcgaccgtc cgtatcccgt gctcgtcgcg
      421 ctgtttttca tcctgtggtt cctcgcggaa acgtttctca actggttgcg catccacgcg
      481 ctgagcgtca gcccgctgcc gctgttcccc cgctacgagg tcaacgaaga cggcgacgaa
      541 tggccgattc agccacgcct gctcaaagtc cgcgagtggc tgcgcgccga agggtttaaa
      601 caaatccagg cgctgaaggc cgagatcggc ggcggaattt atctgcgcgt ttcgatctat
      661 caggaccccg aggcgaccat tcgcgtgcaa gtcaccttca tcccgcaggc gagcggcgcg
      721 atcaccgtgt gctactcgat cgcctcgctg acgagcgacg gcgcgcgcta cgtgaccgac
      781 aatctctacg tgccgttcgc cggcttttat cccgagcact ggttcgtcga gcgcgcgccc
      841 tggcggcgtt cgctcgcgcg cctgcttgcg cggcatcgcg cgcgactctc ccgcactggc
      901 gcggcgctcg agccgttcgc cgacgatccg ttgaccgatc tcaactccac gcaacaagag
      961 ctcgatcgca tcaacaccga cctcggtttc ctccatcaac cgccccaacg cgaagacctc
     1021 ggcaagatca cgcacgaagg ccgctaccgc gtgtggaagg aaatctggat gctcaactac
     1081 ctcggccgcg cgatgcggta tgagtgagca gtgagtagcg gcaggcctcc gcgcctgcct
     1141 gcttggacgc tttgtaggtg gcgcgcttgc gcgcaacgtg gtgcgcaagc gcatcaccta
     1201 cctacccgaa gaaaagcttc gcaacaggcc ggcaggcacg gaggcctgcc gctacgctca
     1261 cccgccgata taactcatct cgatcttctc gcgcgcctcg cccgccgcga gcacctccgc
     1321 ccgctcgtcg ctgtaacgat ccatccggcc gccccaaatc cgcgcgagat aacgctccag
     1381 ctccgcgtcg tccgcgcccg cgcgcaagca cccgagcaca tcccacccga gcgatgcaaa
     1441 caggcacgtg taaagtttcc cgtccgccga caaccgtgcg cgatgacaat cccggcaaaa
     1501 cggctctgtc accgaactga tcagcccgat ctctccacgc ccatcgcgat aacgataacg
     1561 cgccgccact tcgccgcgat acgccggccc gctcgcctcg agcggccatt tctccgcgat
     1621 gcgcgccacg atctcgcgcg ccggcacgac gttgtccggc gtccagtgat tcgtgttgcc
     1681 cacgtccatg aactcgatga accgcaacgg atgcccgcgc tcgcgaaaat actcgcacag
     1741 cgccagcaac tcgccgtcgt tcaccccgcg ctgcaccacc gtgttgatct tcaccgccag
     1801 cccggccgcg gccgccgcgt cgatgccgcg caacacgcgc tccactttga aaccgagtcc
     1861 gttcatccgg cccgccgtcg catcgtccag cgagtcgacg gacacgttga cgcgatcgag
     1921 ccccgcggcc cgtaacgccg gcgcgaattt ctccaagagc cagccattcg tcgtcagcgc
     1981 cacatcgcgg atctgcaact cgctcttcag cacccgcacc aactccgcca catccgcgcg
     2041 caacaacggc tcgccgcccg tcaaccgcac cttctccatg ccgagcgcgc gaaacgcccg
     2101 cacgatccgc acgatctccg ccaacgacat cagttgcgga tcgcgcagaa acgcatgccc
     2161 cggcccaaac acttccttgg gcatgcaata aggacagcgg aagttgcagc gatccgtgac
     2221 ggaaatgcgc aaatcccgca gtgggcggtt gaactggtcg gtcggttgtc ccatgtagaa
     2281 gcccgcttgc aggcgggcgg attgacgtaa ccgcgcccgc aacggtgcgg ccgtccacta
     2341 agcggccgca cctcactgcc ggcaagccgc accctcaaaa tccatgctca cccctgccga
     2401 ggcggataaa ctcatcgccc aaaatctcgc gcctttcctg cgcgaggact gcccgctcgc
     2461 cagcgcacac ggccgcatcc tccgcgaaca cctgcgcgcc gaccgcgatc tgccaccgtt
     2521 cgatcgcgtg acgatggacg gcttcgcgct ccgctccgcc acgttcgccg ccggcacgcg
     2581 cacgtttcgc atcgagggca cgcaagccgc cggcatgcga ccgctgaaac tcagcgccgc
     2641 cgacgaagcc tgcgtcgaag tgatgaccgg cgccgtgctg cccgagggcg ccgattgcgt
     2701 cgtgccttac gaagacacca cacgcgacgg caccacgctc acgatcgccg aagccatttc
     2761 cctcacgccc ggctccgccg tccatcgccg cggcagcgat catcccgcca acgacgtgat
     2821 cgtgcccgcc ggc
//
LOCUS       DASPLT010000015         1903 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_102660, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000015 DASPLT010000000
VERSION     DASPLT010000015.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1903)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1903)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1903
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_102660"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..788)
                     /locus_tag="VEA63_00190"
     CDS             complement(<1..788)
                     /locus_tag="VEA63_00190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009508937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dna2/Cas4 domain-containing protein"
                     /protein_id="HYD82423.1"
                     /translation="MLNEFVYCPRLFYYEHVEGLFRHNADTKRGAALHKRVDAGKGAL
                     PAAAPADGGGSQISDLKSEIPAAQPPEPDVIHSRSVSLSSDRLGVTAKMDLIESRRER
                     EGDLLSPLIVCPVDYKIGSPREGEAGPEIWDADRMQLGLQCLILRDNGYTCTEGVIYY
                     RGTQQRVTLAITAELEQWVLAQIAAARRAMLGPIPPPLVDSPKCARCSLVTICLPDET
                     RLLHEAPSHPDDEDAPPSQEMFGFAREFGAVQELPARYSTGAPDG"
     gene            complement(848..1552)
                     /locus_tag="VEA63_00195"
     CDS             complement(848..1552)
                     /locus_tag="VEA63_00195"
                     /inference="COORDINATES: protein motif:HMM:NF016330.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HYD82424.1"
                     /translation="MGSVVESNEALTATVRGAEIYRVRLAANENEGELAFRCSCPLGR
                     DGEFCKHSVAVALAWLGQHKGGAGVNETAAAVMKLDDVRPWLMEQDKESLANWLMETA
                     ARDEWLREKLLRHVARHESSRDRFQAFRRAIDRATPTRGFFAYRETHGLADRIRNAIE
                     PLAELLTEGHAPAVIELCEHALVRIEKILSNADDSDGELSRCLKDVPRGRRARESGSP
                     FVYIATVSQHGFPFTV"
BASE COUNT          366 a          600 c          587 g          350 t
ORIGIN      
        1 ccatccggcg cgcccgtcga gtagcgcgcc ggcagttctt gcaccgcgcc aaactcccgc
       61 gcgaagccga acatctcctg gctgggcgga gcgtcttcat cgtcagggtg ggaaggcgct
      121 tcgtgaagca gtcgcgtttc atccggcaga cagatcgtca cgagcgagca acgcgcgcac
      181 ttcggcgaat cgaccagcgg gggcggaatc ggcccgagca tcgcgcggcg cgcggcggcg
      241 atttgggcga gcacccactg ctcgagttcg gccgtgatcg cgagcgtcac gcgctgctgc
      301 gtgccgcgat aatagatgac gccctcggtg cacgtgtaac cgttgtcgcg caagatcagg
      361 cattgcaggc cgagttgcat gcgatcggcg tcccagattt cggggccggc ttcgccctcg
      421 cgcggcgaac cgatcttgta atcgacgggg cacacgatga gcggcgaaag caagtcgccc
      481 tcccgctcgc gacggctctc gatcagatcc attttcgccg tcacaccgag gcggtccgac
      541 gagagtgaaa ccgaacggga gtgaatcaca tctggctccg gcggctgcgc tgcgggaatt
      601 tcagatttca aatctgagat ttgagatccg ccgccatccg ctggcgcggc ggcgggcagc
      661 gcgcctttgc cggcgtcgac ccgcttgtgc agcgccgcac cgcgcttcgt gtcggcattg
      721 tggcggaaca atccctcgac gtgctcgtag tagaaaagcc gcgggcaata gacaaattcg
      781 ttgagcatcc gcgcagccaa aggctcctgc ggcgagtctc cggctggcgc ggaaatctca
      841 gtcgtgctca tacggtgaag gggaaaccgt gctgcgaaac tgtagctata tagacgaagg
      901 gcgagcctga ttctcgggct cgccgacctc gcggcacgtc cttgaggcac ctgctcaact
      961 cgccgtcgga atcgtcggca ttgcttagga ttttctcgat tcgaaccagc gcgtgctcgc
     1021 acagctcgat caccgccggg gcatgtccct ccgtcagcag ttcggccagc ggctcgatcg
     1081 cgttgcgaat gcggtcagcg agaccgtgcg tctcgcggta ggcgaaaaat ccgcgggtcg
     1141 gcgtcgcccg atcgatcgcc cgacgaaatg cttgaaatcg atcccgcgac gactcgtggc
     1201 gcgcgacgtg ccgcaggagt ttttcacgca gccattcgtc ccgtgccgcg gtctccatca
     1261 accagttcgc gagtgactcc ttatcctgtt ccatcaacca aggtctcacg tcatcgagct
     1321 tcatgactgc ggctgccgtc tcgtttacgc cggcacctcc cttgtgttgc ccgagccaag
     1381 cgagcgcaac ggccacgctg tgcttgcaga actcgccatc gcgaccgagc gggcaggagc
     1441 agcgaaaagc gagttcgccc tcgttttcgt ttgcagcgag acgaaccctg tagatttccg
     1501 cgcctcggac cgtggccgtc agcgcctcgt tgctctcgac cacggagccc acccgtcctt
     1561 cagtgaaata atttttgcca cgatcgaaaa cggggcggtc gccagactgc gaatacgatg
     1621 aacggtgagg agacgacgca acgctgagcg agcaggcatg aaaaaaaagg cgcggcagaa
     1681 ggcacaacga ggcgggcgct cccgcaagcg ggattatacc gaagcgtcct gacgccttga
     1741 ggcaaagata tcgaacccta aacttcgccc cgtgctcatc ctgctgcgct ggcgagaagc
     1801 gtgaaaagca aggcatcgaa cgcaaacgga gtttccaatt tctcagtttc agccgcggcc
     1861 gtttcgcatt ggctacgaga attcgatttc gtgggctcgc tgc
//
LOCUS       DASPLT010000016         1124 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_225525, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000016 DASPLT010000000
VERSION     DASPLT010000016.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1124)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1124)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1124
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_225525"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..224)
                     /locus_tag="VEA63_00200"
     CDS             complement(<1..224)
                     /locus_tag="VEA63_00200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD82425.1"
                     /translation="MKQMLLNVIHRADRGPSRLETLRFQQDEAHRNGLRTTILVTYSA
                     LFDRDVCEYVAAEKKRDDVEVGLHLHELVC"
     gene            complement(454..>1124)
                     /locus_tag="VEA63_00205"
     CDS             complement(454..>1124)
                     /locus_tag="VEA63_00205"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009512063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HYD82426.1"
                     /translation="RATLSRELAPGLGTLSALPPAESPETTHFSVTDAAGNAVANTYT
                     LNGAYGSGVTVPGIGVLLNNEMDDFTTAAGKPNAFGLIQGEANAIVPGKRPLSSMTPT
                     MVFKDRRLVLVTGSPGGPTIINTVLQVISHVIDDGMPVAQAVEAPRVHHQWLPDVLTY
                     ERYGMSTDTAAALRARGHELKERASYEGAYQGDAATIAIDPNSGVHQGAADPRRPDTR
                     AVGF"
BASE COUNT          200 a          372 c          336 g          216 t
ORIGIN      
        1 tcacacacca gctcgtgcaa atgcagcccg acctcgacgt cgtcgcgctt cttctccgcg
       61 gcgacatact cgcacacgtc gcgatcgaac agcgcgctgt aggtcaccag aatcgtggtg
      121 cgcaaaccgt tccggtgcgc ctcgtcttgt tgaaagcgca gcgtctcgag ccgggagggt
      181 ccccggtccg cgcggtgaat cacgttcaac agcatctgtt tcatcggacg gataaaaccg
      241 cggcagtcag cgcggaaccg gtgcaacctc cttcatcccg ttcaagtcga cgccatgctc
      301 ttcgtggttc ttcggccaga aaccggagaa accgcgcagc catcggcctg gccaaacgcc
      361 gggaaaggat catcagcgcc aaggtggatt catcgaatcg tgttgcgcaa catttttatt
      421 ctgttgccac gttcagtttc gcgcgttcca cgatcaaaag ccgaccgcac gcgtgtccgg
      481 ccggcgcgga tcggctgccc cttggtgcac gcccgagttc ggatcgatcg cgatcgtcgc
      541 tgcgtcgcct tggtaagcgc cttcatagga ggcgcgctcc ttcaactcat gcccgcgcgc
      601 tcgcaacgcc gcggccgtgt cggtcgacat cccataacgc tcgtaggtca gcacgtccgg
      661 cagccactga tgatgaacgc gaggcgcctc gaccgcctga gcgacgggca tgccatcgtc
      721 gattacgtgg ctgataacct gaaggacggt gttgataatc gtcgggccgc caggacttcc
      781 cgtgaccaga accagtcgtc ggtccttgaa caccatcgtc ggagtcatcg acgacagcgg
      841 gcgcttgccg ggcacgatcg cgttggcctc gccttgaatg agaccaaacg cgttgggctt
      901 gcccgccgcc gtggtgaaat catccatttc gttgttcagc agcacgccga ttccgggaac
      961 ggtcacgccc gacccgtaag cgccgttgag cgtatacgtg ttcgcgacgg cgttgcccgc
     1021 cgcatccgtc acgctgaagt gagtcgtctc tggcgattcc gccggcggga gcgcgctcaa
     1081 cgttccgagg cccggcgcca gctcgcggct gagcgtggcg cgct
//
LOCUS       DASPLT010000017         2235 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_164066, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000017 DASPLT010000000
VERSION     DASPLT010000017.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 2235)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2235)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2235
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_164066"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..888
                     /locus_tag="VEA63_00210"
     CDS             <1..888
                     /locus_tag="VEA63_00210"
                     /inference="COORDINATES: protein motif:HMM:NF013694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HYD82427.1"
                     /translation="TPDEIKRVETLLGIELPTRNETQEIEVSSRLYTENRTLFMTATI
                     MVRADKPDLTTAAVTFIYRSQQLVTLRFDSPMPFRTFAARFERNRPTYDTAQKVLLGL
                     FDEVVDRVADILEFVAADLNDLSTTIFMEEPHETGTLNYTNLLARIGRNGARTANARE
                     SLVSLRRLHGFFTEMCSSQQGEELEENWRIAGKDVTALADHATFISSKVNFFLDATLG
                     RVNSEQNTINSQQNKIIKLFSVLAVVLLPPTLIASIYGMNFEFIPELKWKWGYPFSLV
                     LMLISAIVPYLIFRYKRWL"
     gene            complement(958..1206)
                     /locus_tag="VEA63_00215"
     CDS             complement(958..1206)
                     /locus_tag="VEA63_00215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD82428.1"
                     /translation="MNQESKTVLSNNNLRRMQGWARVVYPHDWQDRCAVVAEYLSQQD
                     EDTFGALLKKSWPEVFATVERDWPEAFAGWKSESEPSQ"
     gene            complement(1530..>2235)
                     /locus_tag="VEA63_00220"
     CDS             complement(1530..>2235)
                     /locus_tag="VEA63_00220"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HYD82429.1"
                     /translation="PAHGGSIAFANLIRESCERSLRQLRTKHLDLLICDWDDHLLPVE
                     DVLEAADMLIRAGLVRYVVAGGFPPWRVVDSIHRSGTRNHARFEALQTEYSLMSRVRF
                     EPEALAMCREHRLGFLARSPLAGGFLAQRVALPEPINEDRDWPSERFGSNVGDAVLKV
                     LAEIADKRLATPAQIALAWVLRNTQVTSAVISAQTVHELRELVGASRIVLTGEETGAL
                     ANVSTVQDHRMELRHA"
BASE COUNT          454 a          697 c          674 g          410 t
ORIGIN      
        1 acgcccgacg aaatcaagcg ggtcgagacc ttgttgggca tcgagcttcc gactcgaaac
       61 gaaacccagg agatcgaagt ttcgagccga ctctacacgg agaatcggac gttgttcatg
      121 actgcgacca tcatggtgcg cgcagacaaa ccggatctga cgacggccgc ggtgacgttc
      181 atttatcgat cacagcaact cgtgaccttg cgcttcgatt cgccgatgcc gttccggacc
      241 ttcgccgcga ggttcgaacg caaccggccc acctacgaca ccgcgcaaaa ggtgcttctg
      301 ggcttgttcg atgaagtggt cgaccgcgtc gccgacattc tggaattcgt ggctgccgac
      361 ctgaacgatc tttcgacaac catcttcatg gaagaaccgc acgaaacggg aacgttgaac
      421 tacaccaatc tgctcgcccg catcggacga aacggcgctc gcaccgccaa tgcccgcgag
      481 agtctcgtca gcctgcggcg gcttcacggc ttcttcaccg aaatgtgcag cagccagcaa
      541 ggcgaggagc tcgaagaaaa ctggcgcatc gccggcaaag acgtcaccgc cctcgccgat
      601 catgcgacgt tcatctcgag caaggtgaat tttttcctcg atgccacgct cggccgggtg
      661 aatagcgaac agaacacgat caacagccag cagaacaaga tcatcaaact gttctcggtc
      721 ctcgccgtcg tcctgcttcc gcccacgctc atcgcgagca tctacgggat gaatttcgaa
      781 ttcatccccg agttgaaatg gaaatggggc tatccgtttt ctttggtgct gatgctcatc
      841 tccgcgatcg tgccgtattt gatcttccgt tacaaacgct ggctgtgagc gattggggcg
      901 acacgaagat cacataaaaa aatcctgccc ggtgaggggc aggatcgctg ttctgaacta
      961 ttgactgggt tccgattcgc tcttccagcc ggcaaaagcc tcgggccagt cgcgctcgac
     1021 ggtggcaaac acctccggcc agctcttttt gagcaaggcg ccgaaggtgt cttcatcctg
     1081 ctgcgagaga tactccgcga cgaccgcgca ccggtcctgc cagtcgtgcg gataaacgac
     1141 gcgagcccag ccctgcatgc ggcggagatt attgttactc aaaacggttt tcgattcttg
     1201 gttcatagac gaaaggggcg gtctcgtcgc ctgattgatg taggtgcacg cagcgcatcg
     1261 gtgcgcgcgt gcggttgcgg cgcccgcgcg ttcgcgcgag cgttttcact ggcgataacc
     1321 cggccgatac agcgtctccg ccccgcgcct cgagactagg cgcaagacgc ggagccgctc
     1381 gcccgaccgg ccgcgtccgg gcagacggcg cgaagcatgc cggcatagac gaccgcgccg
     1441 gacgcgaaat cgtgaacggg aacagcgtga tccatgatac cggtcgtgcg catgcggaaa
     1501 tgcgatttcg cattccgctg cgccgctcgt caggcgtgtc gaagctccat gcggtggtct
     1561 tgcaccgtgc tgacgttcgc cagcgcgcca gtttcttcgc cggtgagaac gattcgggaa
     1621 gcgccgacga gttcgcgcaa ctcgtgcacc gtctgcgccg agatgacggc ggacgtcacc
     1681 tgcgtgttac ggagcaccca ggcgagcgcg atctgcgccg gagtcgcgag ccgcttgtcg
     1741 gcaatctcgg ccagcacctt cagcaccgcg tcgccgacat tgctgccgaa acgctcgctc
     1801 ggccaatcgc ggtcctcgtt gatcggttcg ggcagcgcga ctcgttgcgc aaggaagccg
     1861 cccgcgagcg gcgagcgagc gagaaagccg agccgatgct cgcgacacat ggcgagtgcc
     1921 tccggctcga agcgaacgcg gctcatcagc gaatactccg tctgcaaggc ttcgaagcgc
     1981 gcatggttgc gcgtgcccga gcggtgaatg gaatccacca cgcgccatgg cggaaaaccg
     2041 ccggcaacga catagcgcac gagacccgca cggatcagca tgtccgcggc ctcgagcaca
     2101 tcctcgacgg gcagcaagtg gtcgtcccag tcgcagatca ggagatcgag gtgcttggtg
     2161 cgcagttgtc gaagcgaacg ttcgcacgat tcgcgaatga gattcgcgaa ggcgatgctg
     2221 ccgccgtgcg cgggc
//
LOCUS       DASPLT010000018         2029 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_225531, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000018 DASPLT010000000
VERSION     DASPLT010000018.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 2029)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2029)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2029
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_225531"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..698)
                     /locus_tag="VEA63_00225"
     CDS             complement(<1..698)
                     /locus_tag="VEA63_00225"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HYD82430.1"
                     /translation="MRISSFTSWFLDLLRVVAAVGVVLSHLGTTKLAPGVAWLQPFGH
                     LMVVCFFVLSGFLIAASVSGKNVGGARYAALRLGRLWSVAIPCLLVTLLLQLLAENLA
                     PAQFSAFERGHPLLRYGLVAFFLNEVWFSSAGMAFNSPVWSLAYEAWYYALFGVAVFV
                     RNPVWRIGLLAGLALVAGPKILLLFPIWLLGVGLWKVFQWRASLRAWSWPVLVLSVTT
                     IMLWIAVHPRWPAAV"
     gene            915..>2029
                     /locus_tag="VEA63_00230"
     CDS             915..>2029
                     /locus_tag="VEA63_00230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HYD82431.1"
                     /translation="MPRFMIGLRDDTDSLAPPPPPSRAPELAAKIFADDGFLAHGLSL
                     EHRPEQEQMARTVAAAMRDDAPLLFEAGTGVGKSLAYLAPGIIHAVDQSRQMIVSTHT
                     ISLQEQLQTKDLPICRRLFNSAPDLQRYADFKSAVLVGKSNYLCTTRLATALADRASL
                     FADADYDELQRIATWADKTDTGLRHDLKPPPRPEVWDAVNADSSSCSRKHCDCEKCFY
                     QRARARLRAANVVIVNHALLFALINAGGAQANGATTDARGVLFADDFLVLDEAHTVPD
                     VATDNFGLSLSSYGVDRALKYLYNPRTKRGLFRKLGGPEVQQLVVDALDASRLFFDFI
                     ASTLLAQRPIVRVREEGVAEPTLDGPLGALHRLIGKLA"
BASE COUNT          423 a          797 c          482 g          327 t
ORIGIN      
        1 accgccgccg gccagcgcgg atgcaccgcg atccacaaca tgatcgtcgt caccgacaga
       61 acgagcaccg gccacgacca tgctcgcaaa ctcgcccgcc actgaaaaac tttccaaaga
      121 ccgacgccga gcagccaaat cgggaacagc agcaggattt tcggccccgc caccaacgcc
      181 aaaccggcca gcaagccgat tctccacacc gggttccgca caaaaactgc cacgccgaac
      241 agcgcgtaat accacgcctc gtaagccagt gaccacaccg gcgaattgaa cgccatcccc
      301 gcgctgctaa accacacttc gttcaggaaa aaagccacca agccatagcg cagcagcgga
      361 tgtccccgct cgaacgcact gaattgcgcc ggcgccaggt tctccgcgag caattgcaag
      421 agcagcgtca ccaacaagca cggtatcgcc acgctccaca accgtcccag tcgcaacgcc
      481 gcatagcgcg cccctccgac attttttccg ctcacactcg ccgcgatcaa gaatccggac
      541 agcacgaaaa aacacaccac catcaagtgc ccgaatggct gcagccaagc cacgccgggc
      601 gccagctttg tcgtgccaag atgcgacaaa accacgccga cggctgcgac cacccgcagc
      661 aagtcgagaa accacgatgt gaagctagat atcctcacga ggaagaacgg atcagacacc
      721 gacggccctc ccagttctcg cttatttcct caggattcgc ctgaacctct catcgggaat
      781 gcgcgcgtcg tattttcttt tccgttggcg acgcagcgcc cgcgtccgca ttttcccgcc
      841 gcacgccacg ccccaaatcg atgttacgcc gcaatcgaag cgcggtttga cctcacccta
      901 ttctttgcgc tttcgtgcca cgcttcatga ttggccttcg cgacgacacc gacagcctgg
      961 cgcccccgcc accaccctcc cgggcgccag aactcgccgc gaagatcttc gccgacgacg
     1021 gctttctcgc ccacggcctc tccctcgaac atcgccccga acaagagcaa atggcccgca
     1081 ccgtcgccgc cgccatgcgc gatgacgccc cgctcctctt cgaagccggc accggcgtcg
     1141 gcaaatccct cgcctacctc gctcccggca tcatccacgc cgtcgatcaa tcgcggcaga
     1201 tgatcgtctc gacgcacacc atttcccttc aggaacagct ccaaaccaag gatctcccga
     1261 tctgccgccg gctcttcaac tccgcgcccg accttcaacg ttacgccgat ttcaaatccg
     1321 ccgtcctcgt cggcaaatcc aattatctct gcacgacgcg cctcgccacc gcgctcgccg
     1381 accgcgccag ccttttcgcc gacgccgatt acgatgaact ccagcgcatc gcgacgtggg
     1441 cggataaaac cgacaccggc ctccgtcacg atctaaaacc cccgccgcgc cccgaggtct
     1501 gggacgccgt caacgccgac tcgtcctcct gctcgcgcaa acactgcgat tgcgagaaat
     1561 gtttctatca gcgcgcccgc gcccgcctcc gcgccgccaa cgtcgtcatc gtcaatcacg
     1621 cgctgctctt cgccctcatc aacgccggcg gcgcccaagc caatggcgcg acaaccgacg
     1681 ctcgcggcgt gctcttcgcc gacgattttc tcgtgctcga cgaagcgcac accgtgcccg
     1741 acgtcgccac cgacaacttc ggcctttcgc tctccagcta cggcgtcgat cgcgcgctta
     1801 aatatctcta caacccgcgc accaaacgcg gcctcttccg caaactcggc ggcccggaag
     1861 tccagcaact cgtcgtcgac gcgctggacg cctcgcggct cttcttcgat tttatcgcga
     1921 gcacgctgct cgcgcagcgc cccatcgttc gcgtccgcga agaaggcgtc gccgagccaa
     1981 cactcgatgg cccgctcggc gcgttgcacc gcctcatcgg caaactcgc
//
LOCUS       DASPLT010000019         1221 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_266525, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000019 DASPLT010000000
VERSION     DASPLT010000019.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1221)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1221)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1221
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_266525"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(348..>1221)
                     /locus_tag="VEA63_00235"
     CDS             complement(348..>1221)
                     /locus_tag="VEA63_00235"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HYD82432.1"
                     /translation="RIDVWINNAGVGLFGRFTHADMEMHRRVVETNLFGAMNGAAAVL
                     PYFMEQGRGTMITNISVGAFAPVPFAAAYTAGKFGLRGFMAALRQEFADAPGIRLCSV
                     FPAIVDTPGFAHGANVSGARMQPKWMVFPPEKVAATMVDLAMRPRAEVCVGWPARLAK
                     AGYGLAPMAVEKATGTAFRAYMKRAESAERTTGNLFSPSTEPMQPSGGLRGGESDFSA
                     RTGSARNKQRSRALTWAGIAGGGAALIAAALLVRRQAGPHGRRRRSDSAEATRREEVE
                     GWRRTEEAAQLSGV"
BASE COUNT          225 a          473 c          319 g          204 t
ORIGIN      
        1 cacgggatcg acggcatttc cgtcgcgggg tgatggggtg aaggtttgca accgatgcat
       61 ttcaacttcg ctcgtcccgc catgccgtga cgatcgagcg ctggggtaga cgtggggcgg
      121 cccactcgaa gcaggcaaac caccatcccc caactcgcac ccgtcgcgag gctctaactg
      181 tagacgccgc aagaccgaac cttcctaccg tggccgcctg tcgcgaagac gaagttgcga
      241 gccctaacgc gatccacacc gttaccaaac tctgacgcaa ttgcgccaga gtttggtatt
      301 ggcttggaaa ctgcgcggat cgagcaccgc ccctccagag cgggatttca aaccccggaa
      361 agctgcgccg cttcctcggt ccgcctccac ccttcaacct cttcccttcg cgtcgcctcc
      421 gccgaatcac tccttcggcg ccgaccgtgc ggccccgctt gacgccgcac cagcaacgcc
      481 gccgcgatca acgccgcgcc accaccggca atccccgccc aagtcagggc acgcgagcgc
      541 tgcttgttcc ttgcgctccc cgtcctcgcc gaaaaatcgc tttcccctcc gcgcaacccg
      601 ccgctcggct gcatcggttc agtcgacggc gaaaacaggt ttcccgtcgt gcgctccgcc
      661 gactccgctc gcttcatata agcgcgaaac gccgtgcccg tcgctttctc caccgccatc
      721 ggcgccagac catagcccgc tttcgccagc cgtgccggcc agccgacgca cacctccgcg
      781 cgcggacgca tcgcgagatc caccatcgtc gctgcgactt tctccggggg aaataccatc
      841 cacttcggct gcatccgcgc accggatacg tttgcgccgt gcgcgaaacc cggggtgtcg
      901 acgatcgccg ggaataccga acacagccga atccccggcg cgtccgcgaa ctcctgccgc
      961 agcgccgcca tgaacccgcg caacccaaac tttcccgcgg tgtaagccgc cgcaaacggc
     1021 acgggcgcaa acgcgccgac cgaaatgttc gtgatcatcg tcccacggcc ctgctccata
     1081 aaatatggca gcaccgccgc cgcgccgttc atcgcgccga acaaattcgt ctccaccacg
     1141 cgccggtgca tttccatgtc ggcgtgcgta aaccgcccga aaagccccac gcccgcattg
     1201 ttgatccaca cgtcgattcg t
//
LOCUS       DASPLT010000020         3330 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_205377, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000020 DASPLT010000000
VERSION     DASPLT010000020.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 3330)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3330)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3330
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_205377"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            150..1730
                     /locus_tag="VEA63_00240"
     CDS             150..1730
                     /locus_tag="VEA63_00240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HYD82433.1"
                     /translation="MNRRLSDRWKRLVQLYQKEIWQPGQLKQFSLQGVLYAVLRVISI
                     TWTVFSETKALSRAAALSFSSLLGLGPLVALTVLVAGFALDSKDPNLAANTLSRLIYF
                     VAPQVGQYGKLPHNQDEAPPSSEAATTETPANAGAHPRAAKPPSIVDDPSAEMQLNPR
                     LVRMMNNFISQGRSGSAGTVGFFGLVIIVLMLFKTVEDTFNEIWGVRSGRSLLMRIVY
                     YWTILTLGAVLFFASVTLLSASAFLSVFEKAVARMPFSDQVALALQWSLPVFSFTLLV
                     VLLTVFYRIIPNTRVFWRAAFIGAVVVASLLMLNNYLAFLYVRRVIMTESLYGSLGFP
                     LVVMFGLYIFWLYVLIGGQISYAVQNVHFRNSQAAWGHLSESMRERLLLVVLLTICRR
                     FQDCLPPVAASHLGSMIKVPTQILNECLNRLVDLKLITPLPAASEEDSSDLLYQPARP
                     LNRITLLDFKTLDDNFGENPIGSSIDQLDPILVRYNQEVSKIGERAFLQKSLEELLTE
                     YPFDQSRPPFTLGESRPT"
     gene            complement(1998..>3330)
                     /locus_tag="VEA63_00245"
     CDS             complement(1998..>3330)
                     /locus_tag="VEA63_00245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD82434.1"
                     /translation="TTVTFTVVIQRPEISIVINNPANNSVYTLPASQDWCSSSPVSIP
                     FTFTSTITAGSTIDALSAALNGCSVSINKSGLGTATAKGTGTLKVSKPGTYTLSVKGI
                     DTTAGISATTSIKIIVQKAAPPTVTITKPTETTYTTSDCDTLSIPFVFKATSGSSTIT
                     SLSAKLDGKYVKVTSSGLKTANATGSGTLGVKSAGKHVLTVSATDQNGTATSSFTFTV
                     TVKKATPTVKITEPDNNETFTYENGEATPKIPFVIEAETNAGATISSIKASLNNCSVD
                     VSTSGIGSRDARGTGKLNIPGPGTYTLTATTVSGGVSATTKVTFTVKKVEGDSCKPEW
                     RGAIKEGKAHKGGRDVEVKFQLKKKSSRGTTSAVRDNSVKISVCEVNRDGSAGKSRIY
                     SSSHYSINKSDEYCFDHPTDRGDNRYRVEVYCFPNGSNKPKLVGSKEFNTK"
BASE COUNT          568 a         1064 c          929 g          769 t
ORIGIN      
        1 cgcgctgctc gccggcagca cgggtctcta cctttgcacc gtgttcgtga ccttcctgcc
       61 ggtgatcatc gcggctgcgc tcgccgtcgg cggcttcgcg ctcgtgtcgt ggcacttgct
      121 gtcctgagct gcggcagcat ggttccgcca tgaaccgccg gttgtccgat cgctggaaga
      181 gacttgtcca gctctaccaa aaagagatct ggcagcctgg gcagttgaaa cagttttcgc
      241 tgcagggcgt tctttacgcg gtgctgcgcg tgatctcgat cacgtggacc gtgttcagcg
      301 aaacgaaagc gctcagccgc gccgccgcgc tgagcttcag ctcgctgctt ggccttgggc
      361 cgctcgtcgc gctgacggtg ctcgtcgccg gctttgcgct cgacagcaaa gatcccaatc
      421 tcgcagccaa cacgctcagc cggctcatct atttcgtcgc gccgcaagtc ggccaatacg
      481 gcaaactccc gcacaaccag gacgaagccc cgccctcatc cgaagcggcc accaccgaaa
      541 ctcccgccaa cgccggcgct catccgcgcg ctgctaagcc accgtcgatc gtcgacgatc
      601 cgtcggcgga gatgcagcta aacccgcggc tcgttcgcat gatgaacaat ttcatcagcc
      661 agggccgctc cggctccgcg ggcacggtcg gatttttcgg cttggtgatc atcgtcctca
      721 tgctcttcaa gaccgtcgag gacacgttca acgagatctg gggcgtgcgc agcggccgtt
      781 cgctgctcat gcgcatcgtg tattattgga caatcctcac gctcggcgcc gtgctgtttt
      841 ttgcgtccgt gacgctgcta agcgcgagcg cgtttctcag cgtgttcgag aaagccgtcg
      901 cccgcatgcc gttttccgac caggtggcgc tcgcgctcca atggtcgctt ccggtctttt
      961 cgttcacgct gctggtcgtg ctgctgacgg tgttttatcg aatcattccg aacacccgcg
     1021 tattctggcg cgccgcgttc atcggggcgg tcgtcgtcgc ctcgctgctg atgctgaaca
     1081 actatctggc gttcctctat gtccgccgcg tgatcatgac cgagagtctc tacggctcgc
     1141 tcggctttcc gctggtcgtg atgttcggcc tctacatttt ctggctctac gtcctcatcg
     1201 gcgggcaaat cagctacgcg gtgcagaatg tccattttcg caacagccag gcggcgtggg
     1261 gccatctctc ggagtcgatg cgcgagcgct tgttgctcgt cgttctcctc accatctgcc
     1321 gccgctttca agactgcctg ccacccgtcg ccgcatcgca cctcggcagc atgatcaaag
     1381 tcccaacgca gatcctcaac gagtgcctca accggctcgt cgatttgaaa ctcatcacgc
     1441 cgctgcccgc tgccagcgaa gaggactcgt ccgatcttct ctatcaaccc gcccgcccgc
     1501 tcaaccgcat cacgctgctg gatttcaaaa cgctcgacga caacttcggc gaaaacccga
     1561 tcggcagctc gatcgatcag ctcgatccca tcctcgtgcg ctacaaccag gaagtgagca
     1621 aaatcggcga acgcgcgttc ctgcagaaat cgctcgaaga gctcctaacc gaatatccct
     1681 tcgatcaatc gcgaccgcct ttcacactgg gcgaatcgcg acccacgtag ccgcataaat
     1741 cactccgagc cttcatcatc gtcagcattc gtctgcatga aaggctccga acaccttgta
     1801 gcctcctgct gcctccgtgt cggcgctttg tgatctctgt gtaacaaagc catggctaat
     1861 gagagcgcgc tcctgtgctt cgacggatga gatctaacat ctcgcgcaag cggctttcgc
     1921 tttcgcgcgg tccggcccgg gtaaaaaaaa gcgccggctg agttgcagcc ggcgctcgcg
     1981 caggatcaaa cccgcgctta cttcgtgttg aattccttag agccgacgag cttcggtttg
     2041 ttgctgccgt tcgggaagca gtagacttcg acgcgataac ggttatcgcc gcgatcggtc
     2101 ggatgatcga agcaatactc gtccgacttg ttgatcgaat agtgcgacga gctgtagatc
     2161 cgcgattttc cggccgagcc gtcacggttc acttcgcaca cggaaatctt caccgagttg
     2221 tcgcgcaccg cgctggtcgt gccgcggctg gatttcttct tgagctgaaa cttcacctcg
     2281 acgtcacggc cgcctttgtg cgccttccct tccttgatcg caccgcgcca ctccggcttg
     2341 caactgtcgc cttccacttt tttcaccgtg aacgtgactt tcgtcgtcgc gctgacaccg
     2401 ccgctcaccg tcgtcgcggt gagcgtgtag gtgccggggc cggggatgtt caatttgccc
     2461 gtgcctctcg catcgcgaga gccgataccc gatgtcgata cgtcgaccga acaattattg
     2521 agcgatgctt tgatcgacga aatcgtcgcg ccggcgtttg tttccgcctc gatcacgaac
     2581 gggatcttcg gcgtcgcttc accgttttca taggtgaacg tttcgttatt gtccggctcc
     2641 gtgatcttca ccgtcggggt ggctttcttc accgtcaccg tgaacgtgaa ggacgaggtt
     2701 gccgtgccat tctgatcggt cgcactcacg gtcaacacgt gcttgcccgc ggatttgacg
     2761 cccaacgttc cggagccggt tgcgtttgcg gttttcagac cgctcgacgt caccttcacg
     2821 tatttgccgt cgagcttggc ggagaggctg gtgatggtgc tcgaaccgct cgtcgcctta
     2881 aacacaaagg gaatgctcag cgtgtcgcag tccgaagtcg tgtaggtcgt ctcggtcggt
     2941 ttcgtgatcg tgaccgttgg cggcgccgcc ttttggacga tgatcttgat cgaagtggtt
     3001 gcgctgattc ccgccgtcgt atcgatgcct ttcaccgaaa gcgtgtaggt gccgggcttg
     3061 ctgaccttca acgtgcctgt gcctttcgcc gtcgcggtgc cgagacccga tttattgatc
     3121 gaaaccgagc acccgttcag cgccgcgctg agcgcgtcga tcgtcgaacc cgcggtgatc
     3181 gtcgaagtga aagtgaacgg aatgctcacc ggtgacgaac tgcaccagtc ctggctggcc
     3241 ggcagcgtgt agacgctgtt gttcgcggga ttattgatca cgatcgaaat ctccggtcgc
     3301 tgaatcacga cggtgaacgt cacggtcgtc
//
LOCUS       DASPLT010000021         2214 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_184660, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000021 DASPLT010000000
VERSION     DASPLT010000021.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 2214)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2214)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2214
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_184660"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..747
                     /locus_tag="VEA63_00250"
     CDS             <1..747
                     /locus_tag="VEA63_00250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3362 domain-containing protein"
                     /protein_id="HYD82435.1"
                     /translation="AHHVSGRLKVAPEHTSDHVLKVMRKPTFKLFYKFKEKFDRAAKK
                     AGKPQTQIVPYFISSHPGSRPEDMAELALKTKDLGFRLEQVQDFTPTPMTVATEIYAT
                     GVHPYDGANVCVARTPEEKQEQRSFFFWYKPEMKAALRKSMQRLGLGEMARRLIDEKK
                     TTVDVTPPPHITPCGMNAADVANASARSGARLEAARRGAKKPMAGGWSDGGRPKAKGP
                     DVDALLREAGVAVRQAPAAKFFQDKDNDGK"
     gene            complement(902..>2214)
                     /locus_tag="VEA63_00255"
     CDS             complement(902..>2214)
                     /locus_tag="VEA63_00255"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HYD82436.1"
                     /translation="FRHILVDEYQDTNTLQSQIVDKLAAHHCVMAVGDDAQCIYSWRG
                     ANFENIMTFPDRHPGTVIHRIETNYRSTPEILNLANGVLLAQPKGRHFEKELRAARGH
                     SHKPFVVQTMDDREQADFILKRIRSLVEDDGVSLNEIAILYRSHFLALEVQLALSRAG
                     LPYHITSGVKFFERQHIRDLVALLRFVYNPADTQAWQRIAVLLPKIGEKGAQKIYQAA
                     VDHARLVQKNFLDVLDTDDVRSKVAKDARDDWTNFAASLLQVAETMQSAKPADAVQTA
                     IDGWYGDYLKGAFADYLDRLDELKALVGFAQRFDEPQDFLAQIVLLNSETSDRHVDVD
                     AEAIKLTTVHQSKGLEYDIVFLIGLADGQFPGRRSIESGDVEEERRLFYVAVTRARNE
                     LYLTYPKVATRAGPGGMMLTPSRFILELPTDLYEPLRIKRSYGW"
BASE COUNT          423 a          548 c          811 g          432 t
ORIGIN      
        1 gcgcatcatg tgtcggggcg gttgaaggtg gcgccggagc acacgagcga tcacgtgctg
       61 aaggtgatgc ggaagccgac gttcaagctg ttctacaagt tcaaggagaa gttcgatcgc
      121 gccgcgaaga aggcgggcaa accgcagacg cagatcgtgc cgtattttat ttcgtcgcat
      181 ccgggttcgc ggccggagga catggcggag ctggcgttga agacgaagga cctgggtttc
      241 cggctggagc aggtgcagga tttcacgccg acgccgatga cggtcgcgac ggagatttat
      301 gcgacgggcg tgcacccgta tgacggcgcg aacgtgtgtg tggcgcggac gccggaggag
      361 aagcaggagc agcggagttt tttcttttgg tataagccgg agatgaaagc agcactgcgg
      421 aaatcaatgc agcggctcgg gctcggcgag atggcgcggc ggttgatcga cgagaagaaa
      481 acgacggtgg atgtgacgcc gccgccgcat atcacgccgt gcgggatgaa cgccgcggac
      541 gtggcgaacg cgtcggcgag atcgggtgcg cggttggaag cggcgcgtcg cggggcgaag
      601 aaaccgatgg cgggcgggtg gtcggacggt gggcgaccga aagcgaaagg gccggacgtg
      661 gacgcgttgt tgcgggaagc aggcgtggcc gtgaggcaag cgcctgcggc gaaatttttt
      721 caggataagg ataacgacgg aaagtaggca gagcgtttgg ggcgtgtggg aggataggtg
      781 gaacccgacg tcccgttggg ttgaacaaag ccgcggcctt cataggcgct tcaaaatcga
      841 tccgccccaa tctcattcga gccaacccga cggaacgtcg ggttctatct tcaatcccag
      901 ttcaccagcc gtagctgcgt ttgatgcgca acggctcgta gaggtcggtg ggcagctcga
      961 ggatgaagcg gctcggcgtc agcatcatgc cgccggggcc ggcgcgggtc gctacttttg
     1021 gatacgtgag atacagctcg ttgcgggcgc gcgtcaccgc gacgtagaag aggcggcgtt
     1081 cttcttcgac gtcgccggac tcgatgctgc ggcggccggg gaattggccg tcggcgagac
     1141 cgatcaggaa aacgatgtcg tattcgagcc ccttggactg atgcaccgtc gtgagcttga
     1201 tcgcttcggc gtcgacgtcg acgtgacgat cactggtttc gctgttgagc aggacgatct
     1261 gtgcgaggaa atcctgaggt tcgtcgaaac gctgcgcgaa gccgacgagc gctttcaatt
     1321 cgtcgaggcg gtcgaggtaa tcagcgaacg cgcctttcaa gtaatcgcca taccagccgt
     1381 cgatcgcggt ctgcacggcg tcggcgggtt tggcggattg catcgtctcg gcgacttgca
     1441 ggagcgaggc ggcgaaattc gtccagtcgt cgcgcgcgtc tttcgcgact ttgctgcgaa
     1501 cgtcgtcggt atcgaggacg tcgaggaagt ttttctgcac gagacgcgcg tgatcgacgg
     1561 cggcttgata aattttttgc gcgccttttt cgccgatttt cgggaggaga acggcgatgc
     1621 gctgccaggc ttgcgtgtcg gcgggattgt agacgaagcg gagcagagcg acgagatcgc
     1681 ggatgtgctg acgctcaaaa aatttcacgc cgctcgtgat gtgatacggc aggccggcgc
     1741 gggagagcgc gagctgcact tcgagcgcga ggaagtgtga gcggtaaaga atcgcgattt
     1801 cgttgagcga gacaccgtcg tcttcgacga gcgagcggat gcgtttgagg atgaaatcgg
     1861 cctgctcgcg gtcgtccatg gtttggacga caaaaggttt gtgcgaatgg ccgcgggcgg
     1921 cgcggagttc tttttcgaaa tggcggcctt tcggctgcgc gagcaggacg ccgttggcaa
     1981 ggttgaggat ctcgggcgtc gagcggtaat tcgtttcgat gcgatggatg acggtgcccg
     2041 gatggcggtc gggaaacgtc atgatgtttt cgaagttcgc gccgcgccag gagtagatgc
     2101 attgcgcgtc gtcgccgacg gccatcacgc aatggtgcgc ggcgagtttg tcgacgatct
     2161 gggactggag cgtgttggtg tcctggtatt cgtcgacgag gatgtggcgg aagc
//
LOCUS       DASPLT010000022         1569 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_61650, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000022 DASPLT010000000
VERSION     DASPLT010000022.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1569)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1569)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1569
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_61650"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..411)
                     /locus_tag="VEA63_00260"
     CDS             complement(<1..411)
                     /locus_tag="VEA63_00260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008773 - [protein-PII]
                     uridylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF294 nucleotidyltransferase-like
                     domain-containing protein"
                     /protein_id="HYD82437.1"
                     /translation="MSVTPTVLPVDPLAFPDNASSAERLAACKAYLQAHGAELRARHA
                     AGISGLEIAHERSATIDRMLIRLFDHAYALYVRDQGKPPAPVALVALGGYGRGELSPF
                     SDVDLMFLFPAKTKPAAIKSFLEHLTNEILYPLWD"
     gene            663..>1569
                     /locus_tag="VEA63_00265"
     CDS             663..>1569
                     /locus_tag="VEA63_00265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007363514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HYD82438.1"
                     /translation="MAEITKSYEARDVEQKWYAAWLKAKAFQGVVDPARPPYSIVIPP
                     PNVTGVLTMGHVLNNTIQDVLIRRARLEGHSAMWLPGTDHAGIATQTVVERELRKQKK
                     TRHDFGREKFVEKVWEWRHEKGGIILEQLRRLGASCDWDRTAFTMDPEYSQRVLHVFV
                     ELFKRGYIYRGKRMVNWCPVSLTALSDEEVIMKPVNGTMYRVRYELADQPGRFIEVKT
                     TRPETIPGDVAIAVHPDDPRYADVIGKTVRRPIGPAAEIPIVADSAVDKEFGSGALKI
                     TPAHDKVDYEIGLRHKLPVVDVLKPN"
BASE COUNT          302 a          398 c          583 g          286 t
ORIGIN      
        1 gtcccagagc gggtagagga tttcgttggt gaggtgttcg aggaaggact tgatggcggc
       61 gggtttggtc ttcgccggga agagaaacat caggtcgacg tcgctaaacg gactgagttc
      121 gccacggccg tagccgccga gcgcgacgag ggcaacaggc gcgggcggtt taccctgatc
      181 gcggacgtaa agggcgtagg cgtgatcgaa gagccggatg agcatccggt cgatcgtcgc
      241 ggagcgttcg tgcgcgatct cgaggccgga gatgccggcg gcgtggcggg cgcggagctc
      301 ggcgccgtgg gcttgaaggt aggctttgca cgcggcgagc cgttcggcgg aagaggcgtt
      361 gtcgggaaac gcgagggggt cgaccggaag gacggtgggc gtgacggaca tgaaaacggg
      421 aaagtgagtg gataaattcg cggcgcgcga gggggaagtg gcgcggcggc ggtgacggcc
      481 gggttttgtg ttttgggttt tgggttttgt gttttgggtt ctgttctcgg gagttgggat
      541 tgagagggtg tgctgagtcg gggcgtaaag cccctctcac cgttcgggtt gtgtttaacc
      601 acttgcgtcg cgcggcggcg ggcggttttc tgcgcgtttc gacacccgaa ccgaacccga
      661 aaatggccga gatcaccaag agttacgaag cacgcgacgt tgagcagaaa tggtatgccg
      721 cctggctgaa ggccaaggcg ttccaaggcg tcgtcgatcc ggcccgtccg ccctacagca
      781 ttgtgatccc gccgccgaac gtgacgggcg tgctgacgat gggacacgtg ttgaacaaca
      841 cgattcagga cgtgctgatc cggcgcgcgc ggttggaagg acattccgcg atgtggctgc
      901 cgggaacgga tcacgccggc atcgcgacgc agacggtggt cgagcgcgaa ttgcgcaaac
      961 agaagaaaac gcggcacgat ttcgggcggg agaagtttgt ggaaaaggtg tgggagtggc
     1021 gccacgaaaa gggcggcatc atcctcgagc agctgcggcg gctcggcgca tcgtgcgact
     1081 gggaccggac ggcgttcacg atggatccgg agtattcgca gcgcgtgctg catgtcttcg
     1141 tcgagctgtt taagcggggg tatatttatc ggggcaagcg gatggtgaac tggtgcccgg
     1201 tttcgctcac ggcgctgtcc gacgaggaag tgatcatgaa gccggtgaac ggcacgatgt
     1261 atcgcgtgcg ttacgaactg gcggatcagc cgggacgttt tatcgaagtg aaaaccacgc
     1321 gcccggaaac gattccaggc gacgtcgcga tcgcggtgca tccggacgat ccacgttatg
     1381 cggacgtgat cggcaaaacc gtgcggcgcc cgatcggtcc ggcggcggag attccgatcg
     1441 tcgctgacag cgcggtcgac aaggaattcg gctccggcgc gctcaaaatc acaccggccc
     1501 acgataaggt cgactacgag attggcctgc gccacaagct gcccgtcgtt gacgtgctga
     1561 aaccgaacg
//
LOCUS       DASPLT010000023         1180 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_205498, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000023 DASPLT010000000
VERSION     DASPLT010000023.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1180)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1180)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1180
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_205498"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(469..>1180)
                     /locus_tag="VEA63_00270"
     CDS             complement(469..>1180)
                     /locus_tag="VEA63_00270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HYD82439.1"
                     /translation="VILTANNLAGAVKPLSASFELRRGEVLGVAGLVGAGRSELLRAI
                     FGLDPVRRGTVRIAAAIDTATPGDRWAQGVGLLSEDRKSEGLALDLSLTENLTLPRLP
                     AWIRPAALAGETSTWIEKLGVRCSGPGQLAGQLSGGNQQKIALGRLLRNGADVLLLDE
                     PTRGVDIGAKQNIYRLIDDLANSGKAVLMVSSYLPELLGTCDRIAVMCRGVLGPARPV
                     AGCDEHQIMLAATGAAAA"
BASE COUNT          226 a          320 c          429 g          205 t
ORIGIN      
        1 gatgacggcg gtttgctgga ggatgatcgc gaaattatcg aacgtgacga aggtctcgaa
       61 gcgcaacgcg gcgaagagcg tggtgacgag aatcaatccg aagagcggac cggcggaagc
      121 gaacgcgcgg agggcgttgc gcgcgaaagg ggagcgggag gattcaggca tgagcgcggt
      181 aacgagcgga atttttaacc acggatgaac acggactcga tccgcgatct tgaaccacga
      241 atggacacga attcacacga atcttcggac cgtgagtcct gtggttacat tctgttttta
      301 accacggatg aacacagatg gacacggata tgaaagccgc ctcgtggagt ggctgttgcg
      361 gtttgcggtt ctctttaaac gaccacaagt gagcggatga tttacgtgcg catggacggt
      421 ggacggcggg gggcggggaa tccgtgttca tctgtggttg aaaacgcact acgcggcggc
      481 ggcgccggtg gcggcgagca tgatttggtg ttcgtcgcat ccggccacgg gacgcgcggg
      541 gccgaggacg ccgcggcaca tcacggcgat gcggtcgcag gtgccgagca gttcgggaag
      601 ataactgctg accatcagca cggctttgcc ggagttcgcg aggtcatcga tcaaccggta
      661 gatgttttgc ttcgcaccga tgtcgacgcc gcgcgtgggc tcgtcgagca gcagcacgtc
      721 cgcgccgttg cgcagcaggc ggccgagcgc gattttttgc tggttgccgc cggagagttg
      781 tccggcgagc tgaccggggc ccgagcaacg cacgccgagt ttttcgatcc atgtcgaggt
      841 ttcgccggcg agcgcggcgg ggcgaatcca tgcgggcagc cgcggtagcg tgagattttc
      901 cgtcagcgag agatcgagcg cgaggccttc gcttttgcga tcctcgctca gcaggccgac
      961 gccctgcgcc cagcgatcgc cgggcgtggc ggtgtcgatg gctgcggcaa tgcgcacagt
     1021 gccgcgtcgc acaggatcga ggccgaagat cgcgcgcaac aattcgctgc ggcccgcgcc
     1081 gacgaggccg gcgacgccga gcacctcgcc gcggcggagc tcgaaagagg cggagagcgg
     1141 tttgacggcg ccggcgagat tgttcgcggt gaggatgact
//
LOCUS       DASPLT010000024         1355 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_21164, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000024 DASPLT010000000
VERSION     DASPLT010000024.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1355)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1355)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1355
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_21164"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1355)
                     /locus_tag="VEA63_00275"
     CDS             complement(<1..>1355)
                     /locus_tag="VEA63_00275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD82440.1"
                     /translation="TPAGVTVRNIVLDHVSCSWSMDEMASMWGANGAINNVTFLNSIF
                     ASPIMNGEPDASGHSRLRSHSEGTHGFGPLAGPNTSNISMIRNILAFNYGRNPAIANA
                     TAGAQIVNNLVYRPGPWPDSAIRVDNLTVAPHQVSIAGNAIIRHPTPFTMDHTALPDT
                     SVVDPQTLITEARPHPTFYSTGIHISNNVSANGQFFIDDNRLLNPQTNTWLLNDEIVR
                     DRAGTPVTRLSADPYANSGGTVWTPWASADVEANLVMSAGKFPANRDAIDAELIGNIL
                     DREGPFPAGGTFLERFSDLGSDPWAPVSAQNSRPLETPENPGGDDDGDGYTNLEEWLH
                     QMAAAVESAENLPPIANAGHDQSVVVHGDPTALVALDGRASYDLNGNALTYAWSWAGG
                     SANGVMPTVSLPVGQTVVTLTVTDADGASGSDTVLIEVVDVPPPVAFADLNFTYSGEP
                     KP"
BASE COUNT          231 a          424 c          442 g          258 t
ORIGIN      
        1 ggtttcggct cgcccgaata agtgaagttc aaatcggcga acgcgaccgg cggcggcacg
       61 tccaccactt cgattaacac cgtatcgctg ccagatgcgc cgtcggcgtc ggtgacggtg
      121 agcgtcacca cagtctggcc cacgggcagt gacaccgtcg gcatgacacc gttcgccgat
      181 ccgcccgccc acgaccacgc ataggtgagc gcattgccgt tcagatcgta ggaagcgcgt
      241 ccgtcgagcg cgacgagcgc cgtcggatcg ccatggacga cgacggattg gtcgtgcccc
      301 gcgttcgcga tgggcggcag attttccgcg ctctccacgg cggccgccat ttgatgcagc
      361 cactcttcga gattcgtgta gccgtcgcca tcgtcgtcgc cgccgggatt ttccggtgtt
      421 tcgagcgggc gcgagttttg cgcgctgacc ggcgcccacg ggtcgctgcc gaggtcggag
      481 aaacgctcga gaaacgtgcc gcccgcggga aatggacctt cgcggtcgag aatgttgccg
      541 atgagttcag catcgatcgc gtcgcggttc gccggaaact tccccgcgct catcaccaaa
      601 ttcgcttcga catcggcgga cgcccaaggc gtccacacgg tgccgccgga gttggcgtaa
      661 ggatcggcgc tcaggcgcgt caccggcgtg ccggcgcgat cgcgcacgat ctcgtcgttc
      721 aagagccagg tgttcgtctg cggattcagc agacggttgt cgtcgatgaa aaattggccg
      781 ttcgccgaca cgttgttact gatgtgaatg cccgtgctgt agaacgtcgg gtgcggccgc
      841 gcttccgtga tgagcgtctg cgggtccacc accgaggtat ccggcagcgc cgtgtggtcc
      901 atcgtgaacg gcgtcggatg ccggatgatc gcgttgccgg cgatggacac ctggtgcggc
      961 gccacggtga gattatcgac gcggatggcg ctatccggcc aggggcccgg ccggtaaacg
     1021 agattgttca cgatctgcgc gccggccgta gcgttggcga tcgccggatt gcggccgtag
     1081 ttgaacgcca agatatttcg gatcatcgaa atgttcgacg tgttcggccc cgccagcggg
     1141 ccgaagccgt gcgtgccttc cgagtgactg cgcaaacgcg aatgtccgct ggcgtccggc
     1201 tcaccattca tgatcgggct cgcgaaaatg ctgttcaaaa acgtcacgtt gttgatcgcg
     1261 ccgttcgcgc cccacatcga cgccatttcg tccatcgacc agctgcagga aacgtgatcc
     1321 agcacgatgt tgcggacggt cacaccggcg ggcgt
//
LOCUS       DASPLT010000025         1620 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_123261, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000025 DASPLT010000000
VERSION     DASPLT010000025.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1620)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1620)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1620
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_123261"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..996)
                     /locus_tag="VEA63_00280"
     CDS             complement(<1..996)
                     /locus_tag="VEA63_00280"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HYD82441.1"
                     /translation="MNGFLQHFGISLRLHFRNRMALLYGYLFPLIFLAAFWVLYRHET
                     VPLLRHMGELLTVSVLGGACFGLPTTLVSERERGVWRRYRLAPVGTWTLVASTAVARY
                     VIVLTAGALQLAIALAVGMTAPAHALALFVVFTFVAFAFIGLGLVIAMLADNVPAVQA
                     LGQCIFLPMLIIGGVAVPLASLPEWAQHVSAFFPGRYAVEALQACVTGDGFAGVRFEL
                     VALAAIGVAGCVTGAKLFRWDAGQRFGAVSGKAWLVPVLAAWVAVGFWAEARGRVAPI
                     VATEPAQEVAQPSEPEVQPWQLIGEAEIAAIDYKNIPPDNGLVTPMAAPDVVPDDE"
     gene            complement(993..>1620)
                     /locus_tag="VEA63_00285"
     CDS             complement(993..>1620)
                     /locus_tag="VEA63_00285"
                     /inference="COORDINATES: protein motif:HMM:NF024700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HYD82442.1"
                     /translation="LRLFGALYRERASSAELLERFALADKANARFETLSGGQRQRLAL
                     ALAFVNRPELVLLDEPTTGLDPQARRELHEEIRRMKSDGHTVLLTTHYLEEAEQLCDR
                     IAIVANGRLIATGGPRELIARSSASQTIRVATAQPLDRGRFAALPGVDEVAEEGAVVT
                     ARTSDAVETLAGLARMLAEEKAELLELNVRKATLEDVFLGLTKNEGEA"
BASE COUNT          310 a          647 c          429 g          234 t
ORIGIN      
        1 ctcatcgtcc gggacaacat cgggcgccgc catcggcgtc accagcccgt tgtcgggcgg
       61 aatgtttttg taatcgatcg ccgcgatctc cgcctcaccg atgagctgcc acggctgcac
      121 ctccggctcg ctcggctgcg ccacttcttg cgccggttcc gtcgccacga tcggcgcaac
      181 gcgtccacgc gcttccgccc agaagccgac tgctacccac gccgcgagca ccggcaccag
      241 ccacgccttc ccgctcaccg cgccgaagcg ctgccccgcg tcccagcgaa acaatttcgc
      301 tcccgtcaca caccccgcca cgccgatcgc cgcgagcgca acgagctcga agcgcacccc
      361 cgcgaaaccg tcgccggtca cgcacgcttg cagcgcctcg accgcgtaac gcccgggaaa
      421 aaacgccgac acatgctgcg cccactccgg caaactcgcc aacggcaccg ccacgcctcc
      481 gatgatcaac atcggcagaa aaatacactg ccccaacgcc tgcaccgccg gcacgttgtc
      541 cgccagcatc gcgatcacga gcccgagccc gataaacgcg aacgccacga acgtgaacac
      601 gacgaacagc gccagcgcgt gcgccggcgc cgtcattccc accgccagcg cgatcgcgag
      661 ctgcagcgcc ccggccgtga gcacgatcac ataacgcgcg accgccgtgc tcgcgaccag
      721 agtccatgtg ccaaccggcg cgagccggta gcgccgccac acgccgcgct cgcgctcgct
      781 caccagcgtg gtcggcagtc caaaacacgc gccgccgagg acgctgaccg tgagcaactc
      841 gcccatgtga cgcagcagcg gcaccgtctc gtgccgatac aacacccaga acgccgccag
      901 aaaaatcagc ggaaacaaat aaccatagag cagcgccatc cggtttcgga aatgcagccg
      961 caaagaaatt ccgaagtgtt gcaaaaatcc gttcacgctt cgccctcgtt tttcgtcagc
     1021 ccgagaaaca catcttcgag cgtcgctttt cgcacgttca actccagcaa ctccgccttt
     1081 tcctccgcga gcatccgagc cagccccgcg agtgtttcga ccgcatcgct cgtgcgagcc
     1141 gtcaccaccg cgccctcctc cgccacctcg tccactcctg gcagcgcggc gaagcgccca
     1201 cgatcgagcg gctgcgccgt cgcgaccctg atcgtctgag acgcactcga tcgcgcgatc
     1261 aactcccgtg gcccgccggt cgcgatcagc cggccattcg cgacgatcgc gatgcgatcg
     1321 cacaactgct ccgcttcctc cagatagtgc gtcgtcaaca acacagtgtg cccgtcgctc
     1381 ttcatccgcc tgatttcctc gtgcagctcg cgccgcgcct gcgggtcgag cccggtcgtc
     1441 ggctcgtcga gcagcaccaa ttccggccgg ttcacgaacg cgagggccag cgcgagccgc
     1501 tgccgctgcc cgcccgagag cgtttcaaac cgcgcgttcg ctttgtccgc gagcgcgaat
     1561 cgctccagca actccgccga cgacgcgcgc tcccgataca gcgcgccaaa taatcgcaac
//
LOCUS       DASPLT010000026         2565 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_266693, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000026 DASPLT010000000
VERSION     DASPLT010000026.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 2565)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2565)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2565
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_266693"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..425)
                     /locus_tag="VEA63_00290"
     CDS             complement(<1..425)
                     /locus_tag="VEA63_00290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009513489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HYD82443.1"
                     /translation="MSPLVTSYYTRANEMLARAWAANAPTLAKLTPIIGESLARGGVL
                     HTFGSGHSELIAREIIGRAGGLVCINGIIDPTAGFIENLVGYGTKLIERHDRQYQLLP
                     GETIIVISNSGKNSSPLEVAHYARKKGLTVVGLTALAMSS"
     gene            complement(546..920)
                     /locus_tag="VEA63_00295"
     CDS             complement(546..920)
                     /locus_tag="VEA63_00295"
                     /inference="COORDINATES: protein motif:HMM:NF024098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3800 domain-containing protein"
                     /protein_id="HYD82444.1"
                     /translation="MINKAALQAEGFKVKESFYKYACRLVLSNAAVMLNDATVVLDGC
                     GDREFRRQLKSYLCRTVNDPEATTVKIRKLKLQDSRQNNLLQVADMVCGAVARYYSQK
                     DDRLVYRTWLKPREKRVQFWPK"
     gene            complement(917..1174)
                     /locus_tag="VEA63_00300"
     CDS             complement(917..1174)
                     /locus_tag="VEA63_00300"
                     /inference="COORDINATES: protein motif:HMM:NF024098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3800 domain-containing protein"
                     /protein_id="HYD82445.1"
                     /translation="MLVFLDESGDAGLKLAAGSSPYFVVTLVIFENHDVANGVDRTVH
                     RLREKFRFAPGFEFKFAKLDRKHREDFLKEINGFDFCYFRS"
     gene            complement(1187..2122)
                     /locus_tag="VEA63_00305"
     CDS             complement(1187..2122)
                     /locus_tag="VEA63_00305"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009513485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HYD82446.1"
                     /translation="MTLVERPALILADRWQDYQLLDCGDGMKQERWGAYTLVRPDPQI
                     IWPRHATSSGKSGKWESWDGFYHRSESGGGKWEYRRELPDHWQIAYKSLGLTFKIHPT
                     SFKHTGLFPEQAVNWEWFSAKIKAARAGSGATGETLGREISVLNLFGYTGAATVAAAA
                     AGASVCHVDAAEGMVKWCRENAALSGHAAAPIRYITDDCLKFARREIKRGRKYDAIIM
                     DPPTYGRGSTGEMWKLEDHLWELLLECRALLSERPLFFLINAYTARLSPTVVANLLAE
                     LFDGRGGRITAGEVGLPVQRDGKVLPCGIYGRWEA"
     gene            2237..>2565
                     /locus_tag="VEA63_00310"
     CDS             2237..>2565
                     /locus_tag="VEA63_00310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD82447.1"
                     /translation="MNSHLNFARVCAASLIAFSALAALRADVIETKNGARIVGEVVKI
                     SEGTITMKTDYAGELAIKQGEVTAITTDKEITVRLESGTVLQGTLSTEAGSLKITGGD
                     GQLSTNV"
BASE COUNT          488 a          738 c          802 g          537 t
ORIGIN      
        1 gacgacatcg caagtgcggt gagtccgacg acggtcagcc cctttttcct ggcatagtgc
       61 gcgacttcga gcggcgagga gtttttaccc gagttggaga tgacgatgat ggtttcgccg
      121 gggaggagtt gatactggcg atcgtggcgc tcgatgagct tcgtgccgta gccgacgaga
      181 ttttcgatga agccggccgt gggatcgatg atgccgttga tgcaaacgag tccgccggcg
      241 cggcctatga tctcgcgcgc gatgagttcg ctatgaccgc tgccgaaggt gtgcagcacg
      301 ccaccgcggg cgagcgattc gccgatgatc ggcgtgagtt tggcgagcgt cggcgcattc
      361 gcggcccagg cgcgcgcgag catttcgttg gcgcgggtgt aataggaggt gacgagagga
      421 gacataggga cgaaaatcgg gagtcttgat tggtgagacg atgtgtgtat gaatgtgggc
      481 ggattcaaac cccgcgccga actgtcaccg tatggtgagc gtgccctcag ggctaggccg
      541 gggttttatt ttggccagaa ttggacgcgt ttctctcgtg gcttcagcca ggtgcggtaa
      601 acaagacgat cgtctttctg tgagtaatag cgcgcgacgg ctccgcaaac catgtcggcg
      661 acttggagga gattgttctg ccgggagtct tgcagcttta gcttgcgaat cttcaccgtc
      721 gtggcctccg gatcgttcac ggttcggcag agatagctct ttagttgccg cctgaactcc
      781 cggtctccgc agccatcaag caccaccgtc gcgtcgttca acatgacggc ggcattactc
      841 aggaccaaac ggcacgcgta tttgtagaag gactccttta ctttgaagcc ttccgcttgc
      901 agcgccgcct tgttgatcac gatcgaaaat agcaaaaatc gaagccgtta atctctttta
      961 gaaaatcttc gcggtgcttt ctgtcgagct tggcgaactt gaattcgaat cccggcgcga
     1021 agcgaaactt ctcacgcagc cggtggacgg tgcgatccac tccgttggcg acgtcatgat
     1081 tttcgaagat caccagcgtc acgacgaaat acggggacga ccccgcagcc agcttcagcc
     1141 ccgcgtcgcc cgattcgtcg agaaacacca acatgcgaag cagccctcac gcctcccagc
     1201 gtccatagat gccgcatggg agcactttgc cgtcgcgttg caccgggagg ccgacttcgc
     1261 cggcggtgat gcggccgccg cgaccgtcga acaattccgc gaggaggttc gcgacgacgg
     1321 ttggcgagag ccgtgccgtg taggcgttga tgagaaaaaa aagcggccgt tcgctcaaca
     1381 gtgcgcggca ctccagcagc agctcccata aatgatcctc gagtttccac atctcgcccg
     1441 tgctgccgcg gccatacgtg ggcgggtcca tgatgatcgc gtcgtatttg cgtccgcgtt
     1501 tgatttcacg acgcgcgaat ttcaggcagt cgtcggtgat gtaacggatc ggcgccgccg
     1561 cgtgcccgct gagtgcggcg ttttcgcggc accatttcac catgccttcg gcggcgtcga
     1621 cgtggcacac gctcgctccg gcggcggcgg cggcgacggt cgcggcgccg gtatagccga
     1681 agagattcag cacggaaatt tcgcggccga gggtttcgcc cgtcgcaccg gagccggcgc
     1741 gcgcggcttt gattttagcg ctgaaccact cccagttgac tgcctgctcg ggaaacagcc
     1801 ccgtgtgctt gaacgatgtc ggatggattt tgaacgtgag gccgagcgac ttgtaagcga
     1861 tctgccaatg gtcgggtagc tcgcggcgat attcccactt gcctccgccg ctttcgctgc
     1921 ggtgataaaa gccgtcccag ctttcccatt ttccactctt gccgctgctg gttgcatgcc
     1981 gcggccagat gatttgcgga tcgggccgca cgagcgtgta ggcgccccag cgctcttgct
     2041 tcatgccgtc gccgcaatcg agcagctggt agtcttgcca gcgatcagcg agaatcaggg
     2101 cgggacgttc gacgagtgtc atgcgaggga acttgagcca acgccgccag tcatggggtg
     2161 tcgagcgtcg gcatcatttg tgggaagact cccgattgat tgccggcgcg ttttgtctgt
     2221 atcgtttcgc cgacttatga actcacacct gaacttcgcg cgcgtttgcg ccgcttcatt
     2281 aatcgccttc tccgcgttgg ccgctctgcg tgccgacgtg atcgaaacga aaaacggcgc
     2341 caggatcgtc ggcgaagtcg tcaagatttc cgagggcacc atcacgatga aaaccgacta
     2401 cgcgggcgag ctcgcgatca agcagggcga ggtcaccgcg atcaccacgg acaaggagat
     2461 cacggtgcgg ctcgagagcg gcacggttct tcaaggcaca ttgagcaccg aagccggctc
     2521 gttgaaaatc accggcggcg acggacaact ctcgacgaat gtcga
//
LOCUS       DASPLT010000027         1750 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_21228, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000027 DASPLT010000000
VERSION     DASPLT010000027.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1750)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1750)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1750
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_21228"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..468)
                     /locus_tag="VEA63_00315"
     CDS             complement(<1..468)
                     /locus_tag="VEA63_00315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyhepta-2,4-diene-1,7-dioate isomerase"
                     /protein_id="HYD82448.1"
                     /translation="MPQTIRLYSTPQGCVLDVAGRHVRANASFTVDALFASADPYAEA
                     SSAFDSGTPADASAIATLRAPLQSQEIWAAGVTYLRSMAARMEESKDAGGGTFYDRVY
                     VADRPELFFKSTPHRTVAPGAPVRIRADSKWNVPEPELTLAINSRGKIFGYTIG"
     gene            714..1223
                     /locus_tag="VEA63_00320"
     CDS             714..1223
                     /locus_tag="VEA63_00320"
                     /inference="COORDINATES: protein motif:HMM:NF013923.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HYD82449.1"
                     /translation="MPGDSPELMRVTLKLNGVERVVTVEPRAVLLDTLREQLRLTGTK
                     KGCDHGQCGACTLLVNGRRIYSCLTLTAMHDGDDVTTIEGLEQDGELHPMQTAFLDCD
                     GFQCGACTSGQIMSAVGMLNEPVGGDDASIRDAMSGNICRCGAYRGILAAVRQAQEAR
                     ATATAKGGR"
     gene            1223..>1750
                     /locus_tag="VEA63_00325"
     CDS             1223..>1750
                     /locus_tag="VEA63_00325"
                     /inference="COORDINATES: protein motif:HMM:NF013134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HYD82450.1"
                     /translation="MRAFAFSRAENVRDAVARHGVDGHVEYLAGGTTLIDLIKLEVMR
                     PGAVVDVNRIGFTDIQPQPDGGLRIGAMVRNSDLARHPIVRERYPGLAEALLSGASAQ
                     VRNMATTAGNLLQRTRCQYFRDNVSACNKREPGAGCAAMEGYNRSHAVLGGSEACIAT
                     HPSDMCVALAALEASV"
BASE COUNT          323 a          478 c          649 g          300 t
ORIGIN      
        1 gccgatggtg taaccgaaga tttttccgcg cgagttgatg gcgagcgtga gctcgggctc
       61 gggaacgttc cactttgaat cggcgcggat gcgcacgggc gcgccgggag cgacggtgcg
      121 gtgcggcgtg gatttgaaaa acagctcggg gcgatccgcg acgtaaacgc gatcgtagaa
      181 tgtgccgccg ccggcgtctt tcgattcctc catgcgcgcg gccatgctgc gcaaatacgt
      241 gacgcccgcg gcccagattt cctggctttg gagcggagcg cgcaacgtgg cgatcgcaga
      301 ggcatcagcc ggcgtgccgg agtcgaaagc cgacgacgct tcggcataag ggtcggcgga
      361 cgcgaacagc gcatcgaccg tgaaggaagc gttggcccgg acgtggcggc cggcgacgtc
      421 gagcacacaa ccttggggag tggaatagag gcgaatggtc tggggcatgg gaaatgccag
      481 tggaacgcat ccgcgtgcgc cggccagcta caaaaaccgt cagcaaacgc cgcaaacgtg
      541 ctaacaatgc agcaggctgc cattccgcgg ggggttgaga acgaatcatc tccggggtgc
      601 gagccggttc ctcgcctgat gcgcgactgc tggcgaaccg cgaaactgga cgcgaactgg
      661 gggcgcgggc gttggtcggt gctttgtagc gcaagcaacc ctgaattttt tcgatgcccg
      721 gcgattcacc tgagttgatg cgagtgactt tgaaactgaa tggcgtcgag cgcgtcgtga
      781 cggtcgagcc gcgggcggtg ctgctcgaca ccttgcgcga gcaactgcgg ctcacgggga
      841 cgaaaaaagg ctgcgatcac ggacaatgcg gcgcctgcac gctgctggtg aacggaaggc
      901 gcatctactc gtgtctcacg ttgacggcga tgcatgacgg cgacgacgtg acgacgatcg
      961 aagggctgga gcaggacggc gaactgcatc cgatgcagac ggcgtttctg gactgcgatg
     1021 ggtttcaatg cggcgcgtgc acgtcgggcc agatcatgtc ggcggtcgga atgttgaatg
     1081 aaccggtcgg aggggacgac gcgtcgatcc gcgatgcgat gagcgggaat atttgtcggt
     1141 gcggcgcgta tcgcgggatt ctcgcggcgg tgcgccaggc gcaagaggcg cgcgcgaccg
     1201 cgaccgcgaa aggcggccgc tgatgcgcgc gtttgcattc agccgcgcgg agaacgtgcg
     1261 cgatgccgtg gcgcggcatg gggtcgatgg ccacgtcgag tatctggccg gaggcacgac
     1321 actcatcgat ttgatcaagc tggaggtgat gcggccgggc gcggtggtgg acgtgaatcg
     1381 tattggattt acggatatac agccgcagcc ggacggcgga ctgcgcattg gtgcgatggt
     1441 gcgcaacagc gatctcgcgc gacatccgat cgtgcgcgag cgttatccgg ggttggcgga
     1501 agcgctgctg tccggcgcgt cggcgcaggt gcgcaacatg gcgacgacgg cggggaatct
     1561 tttgcagcgg acgcgctgtc agtattttcg cgacaacgta tcggcgtgca acaagcgcga
     1621 acccggtgcg ggctgcgcgg cgatggaagg ttataatcga tcgcatgcgg tgctcggcgg
     1681 cagcgaggcg tgcatcgcga cgcatccgtc ggacatgtgt gtcgcgctcg ccgcgctcga
     1741 ggcgtcggtg
//
LOCUS       DASPLT010000028         1616 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_82272, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000028 DASPLT010000000
VERSION     DASPLT010000028.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1616)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1616)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1616
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_82272"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            91..>1616
                     /locus_tag="VEA63_00330"
     CDS             91..>1616
                     /locus_tag="VEA63_00330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD82451.1"
                     /translation="MKTFAYLRSLVFAALLSPLAAVGAAAWFDYAPTADDERGSAIDL
                     RPLNEPVAGERGFVRVHDGHFALAGSDRPVRFWGVNGPSHHADTPEQLAREARLLAGR
                     GVNLVRVHGALFNDAGNLDEKKVQRWIAIAEAMKREGIYVLYSIYFPLWLKPAPDHPW
                     LHGYNGEQHPFAALMFNPEFQKRYREWWRTLLLTPSRETGRRLIDEPAVLGVEIQNED
                     SYFFWTFSEQNVPPPQREMLERRFGEWLIAKHGSLDAALAAWDGLAFPGDRPLEGRMA
                     FRPLWPIVSEKTARDRDTVAFLLESQRRFYEEQSRFLRELGVKGLITCSNWTTASAEV
                     LGPLEKYSYAIGQFIDRHGYFGSRARGEGAEWSIRAGHTYRDRSALRFDAADGEGARL
                     FVHPSMDPHYAGLPSMISETTWTRPNRYRSEAPLYYAAYGALQDSDAIVHFQFDGSVW
                     SVKPNYWMQPWTLMSPAMMGQFPAAALIYREGLVGKGDVLAKVELGVDRLLALEGTPL
                     P"
BASE COUNT          278 a          457 c          553 g          328 t
ORIGIN      
        1 gctcgcgtcg cacgtcgggc agtcgttgtt ctctgcgtta ttatcatacg aacgtcgccc
       61 ccggctgggg cgcgcatcgg caaagcccct atgaagacct tcgcttattt acgatcgctg
      121 gtgttcgctg cgcttctgtc accgctcgcc gccgtcggcg ccgccgcgtg gttcgattac
      181 gcgccgacgg cggacgatga gcgcggaagc gcgatcgatc tgcggccgtt gaacgagccg
      241 gttgcgggcg agcgcggttt cgtgcgcgtg catgacggtc atttcgcgct cgccggttcc
      301 gatcggccgg tccgcttctg gggcgtcaac ggtccgtcgc atcacgccga cacgccggag
      361 cagctcgcgc gtgaagcgcg actgctcgcc ggacgcggcg tgaacctcgt gcgtgtgcac
      421 ggcgctttat tcaacgatgc ggggaatttg gatgagaaaa aagtgcagcg ctggatcgcg
      481 attgcggagg cgatgaagcg agaggggatt tacgtgctct attccatcta ttttccgctg
      541 tggctgaaac cggctccgga ccatccctgg ctgcacggct acaatggcga acagcaccca
      601 ttcgcggcgt tgatgttcaa tccggaattt cagaagcgct atcgtgagtg gtggcggacg
      661 ttgctgctga ctccgtcgcg agaaaccgga cggcgcttga tcgacgagcc ggcggtgctc
      721 ggcgtcgaaa tccaaaacga ggattcgtat ttcttttgga cgttcagcga gcagaacgtg
      781 ccgccgccgc agcgggagat gctcgagcgg cgtttcggcg aatggttgat tgcgaagcac
      841 ggttcgctcg acgcagcgct cgcggcgtgg gacggactcg cgttcccggg cgatcggccg
      901 ttggaaggac gaatggcgtt tcgcccactg tggccgatcg tgagcgagaa gacggcgcgc
      961 gatcgtgaca ccgtcgcgtt tctgctcgag agccaacgga ggttttacga ggagcagagt
     1021 cgtttcctcc gcgagttggg agtgaaagga ctgatcacgt gcagcaactg gacgaccgcg
     1081 agcgcggagg tgctcgggcc gttggaaaaa tacagttacg cgatcggtca gttcattgat
     1141 cggcacggct atttcggaag tcgtgcgcgt ggcgaaggtg ccgagtggtc gattcgcgcg
     1201 ggccatacgt atcgcgatcg cagcgcgcta cgctttgacg ccgcggacgg ggaaggcgcg
     1261 cggttgtttg ttcacccgtc catggatcca cactacgcgg gattgccatc gatgatttcg
     1321 gagacgacct ggacgcggcc gaatcgttat cgcagcgagg caccgctcta ctacgcggcc
     1381 tatggcgcac tgcaggacag cgacgcgatc gtgcactttc agttcgacgg atcggtgtgg
     1441 tcggtgaaac cgaactattg gatgcaaccg tggacattga tgtcgcccgc gatgatgggg
     1501 cagtttcccg cggcggcttt gatttatcgc gagggtttgg tcggcaaggg cgacgtgctg
     1561 gcgaaggtcg aactcggcgt cgaccgcctg ctcgcgctcg aaggcacgcc gttgcc
//
LOCUS       DASPLT010000029         2538 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_225686, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000029 DASPLT010000000
VERSION     DASPLT010000029.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 2538)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2538)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2538
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_225686"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1470)
                     /locus_tag="VEA63_00335"
     CDS             complement(<1..1470)
                     /locus_tag="VEA63_00335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HYD82452.1"
                     /translation="MIQPAKPDLVTVNIDGKEVAVPKGTNVIEAARLVGVDVPHYCYH
                     PKLSVVGNCRMCLVEMGMPAVDPATKAPIIDPATGKQKINWIPRPQIGCGTNASPGLH
                     IRTTTPLVKDCREGVMEFLLINHPLDCPICDQAGECKLQEQATGYGRGYSRYIEPKNV
                     KPKRTQLGPRVTLDDERCILCSRCIRFCKEVAKDDVLGFIDRGSYTTLTCFPGKQLTN
                     NYSLNTVDLCPVGALTSTDFRFKMRVWFLKQTNSIDTESSVGANTVIWSREGVIYRIT
                     PRRNLEVNDEWMADSGRLLYKQVQSADRLAVAKVNGTDSTLDMALNAAAELFKANAGA
                     IMVVGSGRSSVEEQFLTKKLADALNASTQFVSRVGQGDKLLISADRNPNVRGALVTGL
                     IKALPSAKLTALAAAIDAGKVKTVVAINEDLLAAGLTTEQLAKVAVIYLGTHANATSA
                     VARVVFPTLTVFEKSGTFVNQQFRIQKFNKAIPGPAGTTD"
     gene            complement(1578..>2538)
                     /locus_tag="VEA63_00340"
     CDS             complement(1578..>2538)
                     /locus_tag="VEA63_00340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HYD82453.1"
                     /translation="ARILERAIQEARDKNFVGQNILGTGYSCDIFVHRGAGAYICGEE
                     TGLIESLEGKRANPRIKPPFFPAVLGLYQCPTIVNNVETLCHVKYIAEHGGEAFTKIG
                     TPNNTGTRIFCVSGHVQRPGYYEFEAGKITMGELLYDVCGGPLPGRTFKAVIPGGSSA
                     KIMRFGERYKGKRKVGNDMVDYDWGVEDVPMDFDSLAMVGTMGGSGGVIVMDDTVNMV
                     EALANINAFYSHESCGQCTPCREGSLWMKRITTRMVHGAAREEDAALLKSVADQIPGR
                     TICAFGEACSWPTQSFIAKFGDEFKTYHETKKAPKPTEAAELV"
BASE COUNT          447 a          721 c          843 g          527 t
ORIGIN      
        1 atcggtcgtg ccggccggac caggaatcgc cttattgaat ttctggatac gaaactgctg
       61 attcacgaac gtgccgcttt tctcgaagac ggtcagcgtc gggaaaacga cgcgcgcgac
      121 cgcgctggtg gcgttggcgt gcgtgccgag atagatcacc gcaactttcg cgagctgctc
      181 cgtcgtgagc cccgcggcaa ggaggtcttc gttgatcgcg acgacggttt tcactttgcc
      241 cgcatcgatc gccgcggcga gcgcggtgag cttcgccgag ggcaatgcct tgatgaggcc
      301 ggtgacgagc gcgccgcgga cgttggggtt gcgatcggcg gagatgagga gcttgtcgcc
      361 ctgccccacg cggctcacga attgcgtcga ggcgttgagc gcatcggcga gtttcttcgt
      421 gaggaactgt tcttcaacgg agctgcggcc ggagccgacg accatgatgg cgccggcgtt
      481 cgctttgaag agctccgcgg cggcgttgag cgccatgtcg agcgtgctgt cggtgccgtt
      541 gaccttcgcg acggcgagac ggtcggcgga ctgcacctgt ttgtagagga ggcgtccgct
      601 gtcggccatc cactcgtcgt tcacctcgag attgcggcgc ggcgtgattc gataaatgac
      661 gccttcgcgc gaccaaatga cggtgttcgc gccgaccgac gactcggtgt cgatgctgtt
      721 ggtttgtttg agaaaccaga cgcgcatctt gaaccggaaa tccgtcgagg tgagcgcgcc
      781 gacagggcag agatcgacgg tgttgagcga gtaattgttg gtgagttgtt tcccggggaa
      841 acacgtgagc gtcgtataac tgccgcgatc gatgaagccg agcacgtcgt ccttcgcgac
      901 ctccttacag aagcggatgc agcgcgagca gaggatgcag cgttcgtcgt cgagcgtgac
      961 gcgcgggccg agttgcgtgc gcttcggctt cacgtttttc ggctcgatgt agcgcgagta
     1021 accgcggccg taaccggtgg cttgctcctg gagtttgcat tcgccggctt gatcgcagat
     1081 cgggcaatcg agcgggtgat tgatgaggag aaactccatc acaccctcgc ggcagtcctt
     1141 cacgagcgga gtcgtggtgc gaatgtgcag accgggagag gcgttggtgc cgcagccgat
     1201 ttgggggcgc gggatccagt tgatcttctg cttgccggtg gccggatcga tgatcggcgc
     1261 tttggtggcg ggatcgacgg cgggcatgcc catttcgacg aggcacatgc ggcagttgcc
     1321 cacgaccgag agtttcgggt ggtagcaata gtgcggcacg tcgacaccga cgagacgcgc
     1381 ggcctcgatc acgttcgtgc ctttcggcac ggcgacttcc ttgccgtcga tgttgacggt
     1441 gacgaggtcg ggtttggcag gctgaatcat ttcggaaagt gttgaaccgc taatcttcgc
     1501 taattgccgc taatggaaga gaaggcggac ggaatctgaa aattagcgcg gattagcgaa
     1561 gattagcggt taaaaaatca gaccagttcg gcggcctcgg tgggcttcgg cgctttcttg
     1621 gtttcgtggt aggttttaaa ctcgtcgccg aacttcgcga tgaagctctg cgtcggccag
     1681 gagcacgctt caccgaacgc gcagatcgtg cggccgggaa tctgatcggc gacgctcttc
     1741 aagagcgcgg cgtcttcctc gcgcgcggcg ccatgcacca tgcgcgtggt gatgcgcttc
     1801 atccagagcg aaccttcgcg gcacggcgtg cactggccgc agctttcgtg ggagtagaac
     1861 gcgttgatgt tggcgagcgc ctcgaccatg ttgacggtgt cgtccatcac gatcacgccg
     1921 cccgagccgc ccatcgtgcc gaccatcgcg agcgaatcga aatccatcgg cacgtcctcg
     1981 acgccccaat cgtagtcgac catgtcgttg ccgacctttc gcttgccttt gtagcgctcg
     2041 ccaaagcgca tgattttcgc ggatgatccg ccgggaatca cggccttgaa cgtgcggcca
     2101 ggcagcgggc ctccgcagac gtcgtagagc aactcgccca tcgtgatctt gcccgcttcg
     2161 aactcgtaat aacccggacg ctgcacgtga ccgctgacgc agaagatgcg cgtgccggtg
     2221 ttgttcggcg tgccgatctt cgtaaacgcc tcgccaccgt gctcggcgat atacttcacg
     2281 tggcagagcg tctcgacgtt gttcacgatc gtcgggcact gatacaggcc gaggactgcg
     2341 gggaaaaacg gcggcttgat gcgcgggttg gcgcgcttgc cttcgagcga ttcgatgaga
     2401 ccggtttcct cgccgcaaat gtaagcgccg gcgccgcgat gcacgaagat gtcgcaggag
     2461 taacccgtgc cgagaatgtt ctggccgaca aaattcttgt cgcgcgcttc ctggatcgcg
     2521 cgttcgagga tgcgcgcg
//
LOCUS       DASPLT010000030         2202 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_205616, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000030 DASPLT010000000
VERSION     DASPLT010000030.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 2202)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2202)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2202
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_205616"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..778)
                     /locus_tag="VEA63_00345"
     CDS             complement(<1..778)
                     /locus_tag="VEA63_00345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD82454.1"
                     /translation="MKQFSTLLGAGLSAVIGISLHAAEIAAAQPPYPPSPVIEDIVLD
                     WSTHRREAIGSDNWQLTWADDNQLYGAWGDGGGFGGTNNDGRARLGYARIEGDWDNYR
                     GYNVWGGLDAENPATSNGKSWGTICVDGVLYSWVIPDEPDPTAELNDGFRDHYRYIEL
                     MRSKDHGATWTKASWRWWREDNVMIPTFLVHGRDNDGARDGYVYSYFIRPRDVNTTQR
                     AFKLAVHQPGALFLARVRKEQIFDGRDAYEWFVGREEDGRP"
     gene            complement(775..>2202)
                     /locus_tag="VEA63_00350"
     CDS             complement(775..>2202)
                     /locus_tag="VEA63_00350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD82455.1"
                     /translation="REFGSGFSADVEAMARIGYLYLREGRWRDRQIIPADFVKLAGRP
                     AAESIGLSEYDQSHGNASEHYSLLWWNNGDGTIPGLPRDAFWSWGLYDSLILVIPSLD
                     VVVARAGQSWARTSDEHYDVLKPFFEPIAAAVREFGRPSSDTWSADRKPGGPPYPPSP
                     VIERVVWAPVNEIARRAPGGDNWPMTWGDDDVQYTAYGDGNGFEPFVPEKLSLGFAKV
                     TGSPEDFAGVNSRTATGEFIGDGRSGRKASGLLMVDGVLYVLARNRDNAQLGWSRDRG
                     ATWTWADWRFETSFGCPTFLNYGKNYAGARDDFVYVYSFDNDSAYEPADRMVLARVPK
                     DRIAQRDAYEFFVALDDAGQPEWSRDIAQRGVVFTNPGACFRGGISYDVGLKRYLWCQ
                     ILPHSTHPQGQRFQGGFGVYDAPEPWGPWTTAFYTIDWDVGPGESSSFPTKWMSEDGR
                     IVHLVFSGDDCFSVRKATFVTKEKP"
BASE COUNT          409 a          805 c          608 g          380 t
ORIGIN      
        1 tcggacgccc gtcctcctcg cggccaacaa accattcgta ggcgtcgcgc ccgtcgaaga
       61 tctgctcctt cctcacccgc gcgagaaaca gcgcgccggg ctggtgcacc gcgagcttga
      121 acgcccgctg cgtggtgttc acgtcgcgcg gccggatgaa atacgaataa acgtaaccgt
      181 cgcgcgcgcc gtcattgtcg cgaccgtgca cgaggaacgt cggaatcatc acgttgtcct
      241 cgcgccacca gcgccagctc gccttcgtcc acgttgcgcc gtggtccttc gaacgcatca
      301 gctcgatgta gcggtaatga tcgcggaatc catcgttcaa ctccgccgtc ggatccggct
      361 cgtccggaat cacccacgaa tacagcacgc catcgacgca gatcgtgccc cagcttttgc
      421 cgttcgacgt cgcgggattc tccgcatcga gaccgcccca gacgttgtaa ccacgataat
      481 tgtcccaatc gccttcgatg cgcgcatagc cgagccgcgc gcgaccgtcg ttgttcgtgc
      541 cgccgaagcc accgccatcg ccccacgcac cgtagagctg gttgtcgtcc gcccacgtga
      601 gctgccagtt gtcgctgccg atcgcctcgc gccggtgcgt cgaccagtcc agcacgatgt
      661 cttcgatcac cggactcggc ggatacggcg gttgcgccgc cgcgatctcc gccgcgtgta
      721 acgagattcc aattacggcc gacaagcccg ctccgagtaa cgtagagaac tgtttcatgg
      781 tttttccttc gtgacgaatg tcgctttgcg caccgaaaaa cagtcgtcgc ccgagaagac
      841 gaggtgaacg atgcgcccat cctcgctcat ccatttcgtc ggaaaactgc tgctctcgcc
      901 cggcccaacg tcccagtcga ttgtatagaa cgccgtcgtc cacggccccc acggctcggg
      961 cgcgtcgtaa acgccgaagc cgccctgaaa gcgctggccc tgcggatgcg tgctgtgcgg
     1021 cagaatctgg caccacagat aacgcttcaa ccccacgtcg tagctgatgc cgccgcgaaa
     1081 acaagcgccg ggattggtga acaccacgcc gcgttgcgcg atgtcgcggc tccactccgg
     1141 ttgtccggcg tcgtcgagcg cgacgaaaaa ctcgtaggcg tcccgctgcg cgatccgatc
     1201 cttcggcacg cgcgccagca ccattcggtc cgccggctcg taagcgctgt cgttgtcgaa
     1261 cgaatacacg taaacaaaat cgtcgcgcgc accggcgtag ttcttcccat agttgaggaa
     1321 cgtcggacac ccgaagctcg tctcgaaccg ccagtccgcc cacgtccacg tcgcgccgcg
     1381 atcgcgcgac caaccgagct gcgcgttatc gcggttgcgc gccagcacat aaagcacgcc
     1441 gtccaccatc aggaggccgc tcgccttccg tccggaacgc ccgtcgccga tgaactcgcc
     1501 cgtcgccgtg cgcgaattga cgcccgcgaa atcttccggg ctgcccgtca ccttcgcgaa
     1561 cccgaggctg agtttctccg gcacaaacgg ctcgaaaccg ttgccgtcgc cgtaagcggt
     1621 atattgcacg tcgtcgtcgc cccaggtcat cggccagtta tcgccgcccg gcgcgcgccg
     1681 cgcgatctcg ttcaccggcg cccacaccac gcgctcgatc accgggctcg gcggatatgg
     1741 cggaccgccg ggcttgcgat cggcggacca cgtatccgac gagggccgcc caaactcgcg
     1801 caccgccgcc gcgatcggct cgaagaacgg cttcaacaca tcgtagtgct cgtccgacgt
     1861 ccgcgcccat gattgccccg cacgcgcgac gaccacgtcc aagctcggga tcaccaaaat
     1921 caagctgtcg taaagccccc acgaccaaaa cgcgtcgcgc ggcagcccgg gaatcgttcc
     1981 gtcgccgtta ttccaccaca agaggctgta gtgctcggac gcgttgccat gactctgatc
     2041 gtattccgac aacccaatcg actccgccgc cggacgtccc gccagcttca cgaaatccgc
     2101 cggaataatc tgccggtcgc gccaccgccc ttcgcgcaaa taaagataac cgatccgcgc
     2161 catcgcctcg acgtccgcgc tgaaccccga gccgaattca cg
//
LOCUS       DASPLT010000031         1679 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_246229, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000031 DASPLT010000000
VERSION     DASPLT010000031.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1679)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1679)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1679
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_246229"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(162..>1679)
                     /gene="nirK"
                     /locus_tag="VEA63_00355"
     CDS             complement(162..>1679)
                     /gene="nirK"
                     /locus_tag="VEA63_00355"
                     /EC_number="1.7.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR02376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050421 - nitrite reductase (NO-forming)
                     activity [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper-containing nitrite reductase"
                     /protein_id="HYD82456.1"
                     /translation="NSFLLMAKKLLSLKRWIAFALSGAAVVAQAGEGTVLAKPGAAPL
                     AADLPVEIAMLTKPPLVPPFITRKHPAKVIVHLEVTEETKRLADGVEYTFWTFGSTVP
                     GLFIRVREGDVVEFHLNNHPNSKMPHNIDLHAVTGPGGGAASTFTAPGHSSQFTFTAM
                     KPGLFVYHCATAPVPMHISNGMYGLIFVQPKDGWEPADREYYVMQGDIYTQGTTGETG
                     LQIFDNVKAVDERPTYIVFNGSVGSLVGDSALTANVGETVRLYFGVGGPNITSSFHVI
                     GEIFDRVYVEGGTKTVNENVQTTMVPAGGSTVVDFKVDVPGTYVLVDHSLTRAFNKGA
                     LGMLKVEGPETPVIYSGKEIDEVYLGQASDAGSASSKREAALIAQRAEAIANNPEITN
                     ITQEIQMERGKQVFLTSCFACHMANGEGLPGIFPPLAASDYLKADKNRAIRAVAKGLS
                     GPITVNGKAYNNMMPPQQLDDAQIADVLTYVMNSWGNDYGHVTLDDVKRARSENP"
BASE COUNT          306 a          567 c          478 g          328 t
ORIGIN      
        1 gcacgccgtc accatatttc aaatacgccg ccgatgctcc gcgctccgga ggcgggctga
       61 agcccgccct accgcaaacg cgcgcggatc cacgccagaa tcctgcgtcg tgcaacagcg
      121 tattcatgac tcggctaccg gtttccactt tccccggtcg ctcacggatt ctccgagcgc
      181 gcacgcttca catcgtccag cgtgacgtgg ccgtagtcgt tgccccaact gttcatcaca
      241 taggtgagca cgtcggcgat ctgcgcatcg tcgagctgct gcggcggcat catgttgtta
      301 tacgctttgc cattcacggt gatcggaccg ctcaagcctt tcgcgacggc gcggatcgcg
      361 cgattcttgt ccgccttcag gtagtccgac gcggcgagcg gcgggaagat ccccggcagt
      421 ccttcgccgt tcgccatgtg acaggcgaag cagctcgtga gaaacacctg cttgccgcgt
      481 tccatttgga tctcctgcgt gatattcgtg atctccggat tgttcgcgat cgcctccgcg
      541 cgctgcgcga tcagcgccgc ctcgcgcttg ctcgaagccg agcccgcatc cgacgcctgg
      601 ccgagataca cttcgtcgat ctccttgccc gaataaatca ccggcgtctc cggcccctcg
      661 actttcagca tgccgagcgc gcctttgttg aacgcacgcg tcaacgaatg atcgaccagc
      721 acataggtgc cgggcacgtc gactttgaaa tcgaccaccg tcgagccgcc cgccggcacc
      781 atcgtggtct gcacgttttc gttcaccgtc ttcgtgccgc cctcaacata aacgcgatcg
      841 aaaatctcgc cgatcacgtg aaacgacgaa gtgatgttcg gcccgccgac gccgaagtag
      901 agccgcaccg tttcgccgac attcgccgtc aacgcgctgt cgccgaccag cgaaccgacg
      961 gaaccgttga acacgatgta ggtcggccgc tcgtcgaccg ctttcacgtt gtcgaaaatc
     1021 tgcagaccag tctcacccgt cgtgccctgc gtgtagatgt cgccttgcat cacgtagtat
     1081 tcgcgatcgg ccggttccca cccgtctttc ggttgcacga aaatcaggcc atacatgccg
     1141 ttcgaaatgt gcatcggcac cggcgccgtc gcgcagtgat aaacaaagag gcccggcttc
     1201 atcgcggtga acgtgaactg cgacgagtga cccggcgccg tgaacgtcga cgccgcgccg
     1261 ccgcccggcc ccgtgaccgc gtgcagatcg atgttgtgcg gcatcttcga gttcgggtgg
     1321 ttgttgagat gaaactcaac cacgtcgcct tcgcgcacgc ggatgaaaag tcccggcacc
     1381 gtgctgccaa aggtccaaaa cgtatactcg acgccgtccg cgagccgctt cgtctcctcg
     1441 gtcacctcga ggtgcacgat caccttcgcc ggatgcttgc gcgtgatgaa tggcggcacg
     1501 agcggcggct tcgtcagcat cgcgatctcc acgggcaaat ccgccgccag cggcgccgcg
     1561 ccgggtttgg cgagcacggt gccctcgccc gcctgagcga caacagcggc gcccgacagc
     1621 gcgaatgcga tccagcgttt caaagagagc agttttttcg ccatgaggag aaaggagtt
//
LOCUS       DASPLT010000032         1817 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_225722, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000032 DASPLT010000000
VERSION     DASPLT010000032.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1817)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1817)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1817
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_225722"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..764)
                     /locus_tag="VEA63_00360"
     CDS             complement(<1..764)
                     /locus_tag="VEA63_00360"
                     /inference="COORDINATES: protein motif:HMM:NF025663.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp fold amidoligase family protein"
                     /protein_id="HYD82457.1"
                     /translation="MLPKVLRYRWPTFIRRCIARRLLPFGVGAAPYHRAFRERWRDGV
                     PRTFNEKLQYKLMYDRRKLIAVTADKVAVRDYVQKKTPELKLPKLLAVFDDEAELMAN
                     VPPGPWVMKGAHGAGMVLISKPGATVPREEIRKAAREWMKTDYSVLYWEWQYHEMPRR
                     VLFEEFLGDGTGPPADYKFFVFHRKVRLITVDRGRFGAHTRDLFYPDWTHLPSKKGIA
                     KVSAVPPARPAALERMIAVAEKLGEDTDFVRVDLYL"
     gene            1019..1231
                     /locus_tag="VEA63_00365"
     CDS             1019..1231
                     /locus_tag="VEA63_00365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 family protein"
                     /protein_id="HYD82458.1"
                     /translation="MLKWAIICAIIAVVAGAFGFGAAAGAAAGIAKFLFFLFLVVFFA
                     LLIAALVVGRKVKSALTRDSDRHLKR"
     gene            1356..>1817
                     /locus_tag="VEA63_00370"
     CDS             1356..>1817
                     /locus_tag="VEA63_00370"
                     /inference="COORDINATES: protein motif:HMM:NF016214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HYD82459.1"
                     /translation="MDPLTHTLLGASLGYVTFGRRLGRTAAAVGGLAAFVPDADVFIR
                     SATDPLLAVEFHRHFTHSLVFAPIGAALIASLWLWRKNHRVHWLTIYACALCAYFSHC
                     LLDAATSYGTLLFWPLTRHRFGWDFISVIDLFVTLPLAIGLTWTLLRRRLQP"
BASE COUNT          290 a          717 c          451 g          359 t
ORIGIN      
        1 atcagataaa gatccacccg cacgaagtcc gtgtcctcgc cgagcttctc cgccaccgcg
       61 atcattcgct ccagcgccgc cggtcgcgcc ggcggcaccg ccgacacctt tgcgatccct
      121 ttcttcgacg gcagatgcgt ccaatccgga taaaacaaat cccgcgtgtg cgcaccgaac
      181 cgccctcgat ccaccgtgat cagccgcacc tttcggtgaa aaacgaaaaa cttgtaatcc
      241 gccggcggcc cagtgccgtc gcccaaaaat tcttcgaaga gcacgcgccg cggcatctcg
      301 tggtattgcc attcccaata cagcaccgag tagtccgtct tcatccactc gcgcgcggcc
      361 ttccggatct cctcgcgcgg caccgtcgca cccggcttcg aaatcaacac catgcccgcg
      421 ccgtgcgcgc ctttcatcac ccacggtccc ggcggcacgt tcgccatcag ctccgcttca
      481 tcatcgaata ccgccagcaa cttcggcagc ttcaactccg gcgttttttt ttgcacgtaa
      541 tcgcgcaccg ccactttgtc cgccgtcacg gcgatcagct tccgccggtc atacatcagc
      601 ttgtattgca gcttctcgtt gaacgtccgc ggcaccccat cgcgccagcg ttcgcgaaac
      661 gcgcgatgat acggcgccgc gcccacgcca aagggcaaca atcgccgcgc aatgcagcgg
      721 cgaataaacg tcggccaacg ataacgcagc accttgggaa gcatggataa agcagagaag
      781 ggccgccttt gaaccgcccc gcccgccacg gttccccccg acgatccctt tgcccccaaa
      841 gatccgtcgc aggactgccg cttgcggcat gcctccgagc aatcgcctgc agcgtttcaa
      901 tcgacgcacc cccgcgcccc ccgggcggga acgcccgccg ccacgcccgg tccatcgccg
      961 cggtttcacc acgccacgcg cgctccgttc gcgcctcacc ctcgtccccc tcaccaccat
     1021 gctcaaatgg gccatcatct gcgccatcat tgccgtcgtc gccggcgcct tcggcttcgg
     1081 tgccgcggcc ggcgccgccg ccggcatcgc gaaatttctc ttttttcttt ttctcgtcgt
     1141 tttcttcgcg ctgctgattg ccgcgctcgt cgtcggtcga aaagtgaaat ccgccctcac
     1201 gcgcgactcc gaccgccacc tgaaacgcta gccgcaacaa cgctggtctc acgatttcct
     1261 cgcagccctg cgcctgagcg cggggagttc gcccgcgtcg gctacatcgg ctcacgtggt
     1321 tgccacgctc cgcgcgatgc gtttcatccg ctcgcgtgga tccgctcacc cacactcttc
     1381 tcggcgcgag tctcggctac gtcacgttcg gacgccgcct cggccgcacc gccgccgccg
     1441 tcggcggact cgccgccttc gtccccgatg ccgatgtgtt tatccgcagt gccaccgacc
     1501 cgctgctcgc cgtcgaattt caccggcact tcactcactc gctcgtgttc gcgcccatcg
     1561 gtgccgcgct cattgcctcg ctttggctct ggcgaaaaaa ccaccgcgtc cactggctca
     1621 cgatctacgc ctgcgcgctc tgcgcttact tcagccactg cctcctcgac gccgctacga
     1681 gctacggcac gttgcttttc tggccgctca cccgccatcg cttcggctgg gatttcatct
     1741 ccgtcatcga tctcttcgtc acgttgccgc tcgcgatcgg attgacttgg acgctcctcc
     1801 gccgccgcct ccaaccc
//
LOCUS       DASPLT010000033         3627 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_102863, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000033 DASPLT010000000
VERSION     DASPLT010000033.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 3627)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3627)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3627
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_102863"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(6..1739)
                     /gene="fliD"
                     /locus_tag="VEA63_00375"
     CDS             complement(6..1739)
                     /gene="fliD"
                     /locus_tag="VEA63_00375"
                     /inference="COORDINATES: protein motif:HMM:NF018852.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HYD82460.1"
                     /translation="MAGISISGLVSGTFDWKAVVDQLIQIESAPIARLQTEEAANIDR
                     LASFAALETRLADLKAASTALAAPGLFGGRTASSGTASSDWTMSAATDAPTGSHTIAV
                     SQLATASRRNGASGISAPLSASDDVSGLTLATMPTATAITAGNFTINGKQVAVELTDS
                     LQDVFDKISTATGGVVTASYSSATDKITFASNDDSEIVLGAANDSTNFLVATRLGNNG
                     TDSISSSATLGTASQAVPLADSRLEGIGGIAATGTFTIKGVEIDYDLAVDSLSTLMTR
                     INDSAAGVTASYDSTLDRMVLVNDSTGDVGLGTAEGSSGLLDVLGLGGASTLVRGKNT
                     LFTVDGGATLSSLSTTLDSSAHGIAGLSVTVNSASTQNVTVKADTASMNSAISNFITK
                     FNTVQSYIESQTKVTVGVDGKVNAALLADNREVQGWASEMRRLAFGELSGVAGTVKRL
                     EHLGIDFSSIDSTLRIRDQSKLDAALASKSGDVAAFFNTTGAGFAATFEGYLTPKLDT
                     SNGALKLQMDTLNKQNASIDAQIATLNARLANQRELLTASFLAMQNAQSVAQQQQQQL
                     TNMFASKNDKN"
     gene            1953..2792
                     /locus_tag="VEA63_00380"
     CDS             1953..2792
                     /locus_tag="VEA63_00380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HYD82461.1"
                     /translation="MSVVINTNYSATVASNNLAASNASLQKSLNRLSSGSKIVSPADD
                     AGGLAVSMKLSAAARRQGAVASNIGNAISLLQSQDGAMKVTAKVLERISELKVLYNDV
                     TKSSSDKANYDAEFVALKAQLTSNASEKFNGVTMFGSGVSAGQTGFIGSVSVTEDGLT
                     SVTIAARDLTHSSSGVGAITSTSVTSLGGLDLADVSTAIQNVATMRATNGSEQSRLNF
                     AAELVTVNKANLESATSRIVDVDVASESTQLARWNILVQAGTSMLAQANQSSQTALKL
                     LQG"
     gene            3040..>3627
                     /locus_tag="VEA63_00385"
     CDS             3040..>3627
                     /locus_tag="VEA63_00385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HYD82462.1"
                     /translation="MSVVINTNYAATLASNNLAASNANLQKSLNRLSSGSKIVNPSDD
                     AGGLAVSMKLSAAARRQGAVASNIGNAVSLLQTQDGALKVAAKVLERISELKVLHSDV
                     TKSTSDKANYDSEFTALKTQLTDIAGEKFNGVALFGSSAVGNVNVTEDGATSVSIAAR
                     DLSSTGSGVGQITSTAATSLGSVQLAEVTTAIQNVA"
BASE COUNT          717 a         1160 c         1083 g          667 t
ORIGIN      
        1 cgcactcagt tcttatcgtt cttcgacgca aacatgttcg tcagctgctg ctgctgctgc
       61 tgcgcgacgg actgcgcgtt ctgcatggcg aggaaagacg ccgtcaggag ttcgcgctga
      121 tttgcgagcc gggcgttcaa cgtcgcgatc tgcgcgtcga tgctcgcatt ctgcttgttc
      181 agcgtatcca tctgcagctt caacgcgcca ttcgacgtgt cgagtttcgg agtaaggtaa
      241 ccttcgaacg tcgctgcaaa acccgcgcct gtcgtgttga agaacgcggc gacgtcgccg
      301 ctcttgctgg cgagagcggc atcgagtttc gactggtcgc gaatacgcaa ggtcgagtcg
      361 atcgaactga aatcgatgcc caggtgctca agccgcttga cggtgcccgc gacgccggaa
      421 agttcgccga aggcgagccg gcgcatctct gaagcccagc cttgcacctc gcgattgtcg
      481 gcgagcaacg cagcgttgac cttgccgtcg actccgacgg tgaccttcgt ctgcgactcg
      541 atgtagctct gcacggtgtt gaacttggtg atgaagttcg aaatcgccga attcatcgaa
      601 gccgtgtccg ccttcaccgt gacgttctgc gtgctggcgg aattcaccgt caccgaaagt
      661 ccggcgatgc cgtgtgcgga cgagtcgagc gtggtgctca agctcgaaag cgtcgcgcca
      721 ccgtcgaccg tgaagagtgt gttttttccg cgaacgagcg tcgacgcacc gccgagccca
      781 agcacgtcga gcaaaccgct cgagccttcc gcggtgccga gacccacgtc gccggtgctg
      841 tcgttgacca gcaccatgcg gtcgagcgtg ctatcgtagc tcgccgtgac gccagccgcc
      901 gagtcgttga tccgcgtcat caacgtcgaa agcgaatcga ccgccaggtc gtagtcgatc
      961 tcgacgcctt tgatcgtaaa agtgcccgtc gccgcaatgc cgccgatccc ttcgaggcgc
     1021 gaatcggcaa gcggcacggc ctgcgaagcg gtcccgagcg tagcggaact gctgatactg
     1081 tcggtgccgt tgttgccgag gcgcgtggcg acgagaaaat tggtgctgtc gttggcggcg
     1141 ccgagcacga tctcggagtc gtcgttgctc gcgaaagtga ttttgtccgt cgccgacgaa
     1201 tagctggcgg tgacgacgcc gcccgtcgca gtggaaattt tatcgaagac gtcctgcagc
     1261 gaatcggtga gctccacggc cacctgtttg ccgttgatcg tgaaattgcc ggcggtgatc
     1321 gcggtcgccg tcggcatcgt ggccaacgtg aggcctgaga catcgtcgga ggcgctcaac
     1381 ggcgcggaga tgccgctcgc tccattgcgg cgcgacgcgg tggcgagctg cgaaaccgcg
     1441 atcgtgtggc tgccggtcgg cgcatcggtc gccgcggaca tcgtccagtc cgagctcgca
     1501 gtgccggaag aagcggtgcg tccaccgaag agaccgggtg cagcgagcgc cgtcgatgca
     1561 gcctttagat cagcgaggcg ggtttcgagt gcggcgaagc tggcgagccg gtcgatgttg
     1621 gccgcttctt ccgtctgcaa gcgcgcgatc ggtgcgcttt caatctggat gagctgatcc
     1681 acgaccgcct tccagtcgaa ggtgcctgaa accagtccgg agattgagat gccagccatg
     1741 agaagaagaa acaacgaatc aacagggggt atcggcacga tccgccaaaa cttaagccgc
     1801 cggatatttt cgagaattca gctaaataga acttgacccg acccgatatc agcacccgcc
     1861 gagacaaacg tcgcggccgg cgagaccaga agccgatccc atctggtccg aatgcgacac
     1921 ggacgtcgcg cttagtatca aggaagatca ccatgtcagt tgtcatcaac accaactatt
     1981 cggcgaccgt tgcctccaac aacctcgccg cttcgaatgc cagcctgcaa aagagcctga
     2041 atcgtctctc gagcggctcg aaaattgtca gccctgcgga cgacgccggt ggcttggccg
     2101 tatccatgaa gttgtccgcc gctgcgcgcc gccaaggcgc cgtcgcgtcg aacatcggca
     2161 atgccatctc gctcctgcaa tcgcaggacg gcgcgatgaa ggtcaccgcc aaggtcctcg
     2221 agcgtatcag cgagctcaag gtcctctata acgacgtcac caagagctcc agcgacaagg
     2281 ccaactacga cgccgagttc gtggccctca aggcccagct cacgtccaac gcctcggaaa
     2341 agttcaacgg cgttaccatg ttcggttccg gtgtgtccgc cggccaaacc ggcttcatcg
     2401 gctcggtcag cgtgaccgag gacggtttga cgagcgtcac gatcgcggcc cgcgatctca
     2461 cccacagctc ctccggagtc ggtgcgatca cctccacgag cgtcacgtcg ctcggcggcc
     2521 tcgacctcgc ggacgtttcg accgcgattc agaacgtggc gaccatgcgt gcgacgaacg
     2581 gctccgaaca gagccggctc aacttcgccg ccgaactcgt cacggtgaac aaggccaacc
     2641 tcgagtccgc caccagtcga atcgtcgacg tggatgtggc ttcggagtcc acccagctcg
     2701 cgcgatggaa tatcctcgtg caggccggca cttcgatgct cgcccaggcg aaccagagtt
     2761 cgcagacggc gctgaagctc ctccagggat aaagcgtccg accctcccca ccggtagagc
     2821 ggactagtca tccgccctcc ggcgccagat tgcacgcggt tgtctgatct gcggggccca
     2881 gggtcttagc tcagtcaaag gttcttcatc tcccataacc catttgcgcg gtaacacgcg
     2941 cacacaattt gaggcatttg cctctgacgg cgcggcctac gccgactatc agggcccgtg
     3001 gcgacgacgg atagtcgcac taagatcaag gatagaacca tgtcagtcgt gattaatacc
     3061 aactacgcgg cgacgcttgc gtcgaacaac ctcgccgctt cgaacgccaa cctgcaaaag
     3121 agcctcaacc ggctctccag cggttccaaa atcgtcaacc cttccgatga tgcgggcggc
     3181 ttggcggtgt cgatgaagtt gtccgccgca gcccgccgtc aaggcgccgt ggcgtccaac
     3241 atcggcaacg ccgtttcgct cctccagacc caggacggcg ccctcaaggt cgccgccaag
     3301 gtcctcgagc gcatcagcga gctcaaggtt ctccactcgg acgtgaccaa gagcacgagc
     3361 gacaaagcca actacgattc ggagttcacc gctctgaaga cccagttgac cgacatcgcc
     3421 ggagaaaagt tcaacggcgt cgccctcttc ggctcttcgg ccgtcggcaa cgtcaacgtg
     3481 acggaagacg gtgcgacgag cgtcagcatc gccgcacgcg acctgtccag caccggttcc
     3541 ggtgtcggtc agatcacgtc cacggccgct acctccctcg gtagcgtgca gctcgcggaa
     3601 gtcaccacgg cgatccagaa cgtggcg
//
LOCUS       DASPLT010000034         1404 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_21388, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000034 DASPLT010000000
VERSION     DASPLT010000034.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1404)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1404)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1404
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_21388"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            200..1102
                     /locus_tag="VEA63_00390"
     CDS             200..1102
                     /locus_tag="VEA63_00390"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing response regulator"
                     /protein_id="HYD82463.1"
                     /translation="MIRKILLIDDDRLQFRLTQAHFKNFHGERFELEWAETYEEGIAR
                     LLSGDFAACLLDYQLGPRNGLELIRKAVSVGCRTPIVFLTAESSERVDIEAMNCGALD
                     YLVKGEISSRSLERSLRYALKLGETLEALRRLATRDQLTGLLNRREFERILTEEEERW
                     QRFGHPVALVLVDIDRFKSINDTHGHPVGDAVLREVARRLSEVVRSVDRVARFGGEEF
                     ALIIMESDGAAALETARRACKEIDRQPIMARDTLPLNVTVSAGAASLPTDAKCGETLV
                     TAADRALYAAKAAGRNCALSFAAL"
     gene            1174..>1404
                     /locus_tag="VEA63_00395"
     CDS             1174..>1404
                     /locus_tag="VEA63_00395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007364276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl transferase family protein"
                     /protein_id="HYD82464.1"
                     /translation="MTGCTAVGKTEWALRWAEARGAEIISCDSLLFYRGMDIGTAKPT
                     AAERSRVVHHLIDVCEVTEQMNITSYVKQVREV"
BASE COUNT          241 a          347 c          546 g          270 t
ORIGIN      
        1 cgtttcgcta cgacgaacgg ctacaaggat cgggcacgtg tttcgctacg acgatggtgc
       61 cgcctcccag caagccgggt tctggtgcga gccatggctt gcgcagcgtg gcgcgcgacc
      121 taacatcgcg cgcacttccc atgcgttttc cacgaagcgc cggttttcag ctacgatcaa
      181 ctcacggcgc ccgactgcga tgatacggaa aattcttctg atcgacgacg accgtttgca
      241 gttccggctg acgcaggcgc attttaagaa ctttcacggc gagcgattcg agctggagtg
      301 ggcggagact tatgaagagg gaatcgcgcg gctgctgtcg ggggatttcg cggcgtgttt
      361 gttggattat caactgggcc cgcggaacgg gctggagttg attcgaaagg cggtgtcggt
      421 gggatgccgg acgccgatcg tgtttttgac ggcggagtcg tcggagcgcg tcgacatcga
      481 agcgatgaat tgcggcgcgc tggattatct ggtgaagggg gagatttcgt cgcggtcact
      541 cgagcgctcg ttgcgttatg cgttgaagct gggcgagacg ctggaggcgc tgcggcggct
      601 cgcgacgcga gaccagctca cgggcttgtt gaatcggcgc gagttcgagc ggattttgac
      661 ggaggaagag gagcgctggc agcgattcgg acatccggtg gcgctggtgt tggtcgacat
      721 cgaccggttc aaatcgatca atgacaccca tgggcatccg gtgggcgacg cggtgttgcg
      781 cgaggtggcg cggcggctgt cggaggtggt gcgaagcgtc gaccgcgtgg cgcgtttcgg
      841 cggcgaggaa tttgcgttga tcatcatgga gtcggatggc gcggcggcgt tggagaccgc
      901 gcggcgagcg tgcaaggaga tcgatcggca gccgatcatg gcgcgagaca cgctgccgtt
      961 gaatgtgacg gtgagcgcgg gcgcggcgtc gctgccgacg gatgcgaaat gcggcgagac
     1021 gctggtgacg gcggcggatc gggcgctgta tgcggcgaag gcggcgggga gaaattgtgc
     1081 gctgtcgttt gcggcgttgt gagcggacgg ccgagggtgc aaccacggat gaacacggat
     1141 caacacggag cggcgctgcc tgtgcttcgc gcgttgacgg gctgcaccgc ggtcgggaag
     1201 accgagtggg cgctgcgttg ggcggaggcg cgaggggcgg agatcatctc gtgcgattcg
     1261 ctgctgtttt atcgcgggat ggacatcggc acggcgaagc cgacggcggc ggagcggtcg
     1321 cgggtggtgc accatttgat cgatgtgtgc gaggtgacgg agcagatgaa tatcacgtct
     1381 tacgtgaaac aggtgcggga ggtg
//
LOCUS       DASPLT010000035         1355 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_123423, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000035 DASPLT010000000
VERSION     DASPLT010000035.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1355)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1355)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1355
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_123423"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..334)
                     /locus_tag="VEA63_00400"
     CDS             complement(<1..334)
                     /locus_tag="VEA63_00400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD82465.1"
                     /translation="MNAVKPRSGIVSFRPGEVWLDSSGQPINAHGGGVLHHEGTYYWF
                     GEHKVGGPVGNFAQVGVHVYTSTDLCEWHDAGIALAVSGDPSSEITRGCIIERPKVIF
                     NAATRRFVM"
     gene            645..>1355
                     /locus_tag="VEA63_00405"
     CDS             645..>1355
                     /locus_tag="VEA63_00405"
                     /inference="COORDINATES: protein motif:HMM:NF014376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family ligand binding domain-containing
                     protein"
                     /protein_id="HYD82466.1"
                     /translation="MDAPNYFRYFLTGPESEGWGLAVTATGFTRVPPGTPYPPSAHPS
                     DHHFTWERGRVLEALQIVLIERGEGVFESDGTKPERLVAGTAFALVPQRWHRYRPDPQ
                     TGWTESWIELQGPLVQRWMQHGLFRANRLLRVGADLTGMGRALAEVHAFARGHAGGFD
                     PELAAKAFAVVAAWSRVDGRIVAPTRLQTAIEQAERYLVEQSHAPIDLHQLAQSLGVA
                     YSHFRRAFRKKTGYAPWQY"
BASE COUNT          249 a          474 c          394 g          238 t
ORIGIN      
        1 acatcacgaa gcgtcgcgtc gctgcgttga aaatcacctt cggtcgctcg atgatgcagc
       61 cgcgcgtgat ctcgctgctc ggatcgcctg acaccgccag cgcaattccc gcatcgtgcc
      121 attcgcaaag atcggtcgac gtgtaaacgt gcacgccgac ctgcgcgaaa tttccaacgg
      181 ggccgccgac tttgtgctca ccgaaccagt aataagttcc ctcgtggtgc agcacgccgc
      241 cgccatgcgc gttaatgggc tggcccgacg aatcgagcca aacctcaccg ggacggaacg
      301 acacgatgcc cgaccgcggc ttgacagcat tcatgctgcg tcaactcgag cgagcgttgg
      361 cacaagcccc gagaagcaca cacgcatctc gccgcccgcc ggcacggacg ccggatacag
      421 agaggcgcgc ggccagtttg ccgacgctcg caaaagactc catgaacgca tccctcatga
      481 atcagcgtgt ccgccgaccg cgtgcaatga gcccggcgct taccggttaa gcagacacgc
      541 aacgcgactt cgccgggcga atccccactc gaccggcact ctcgccgatc gaagttgaac
      601 cgccgcattt ctcaccaccc aatatcgctc attcggaaac ggccgtggat gctccaaact
      661 atttccgtta ttttctgacc ggccccgaat cggaaggctg gggactggcg gtcaccgcca
      721 ccggctttac ccgcgtcccg ccgggcacgc cctatccgcc gtccgcccat ccctccgacc
      781 accatttcac ctgggagcgg ggccgtgttc tcgaggcgtt gcagatcgtt ttgattgaac
      841 gcggcgaggg cgtctttgaa tccgatggga ccaaacccga gcggctcgtc gccggcacgg
      901 cgttcgccct ggtccctcaa cgctggcatc gctatcgtcc cgatccacaa accggctgga
      961 cggaaagctg gatcgagtta cagggaccgc tcgtgcagcg ctggatgcag cacggcctgt
     1021 ttcgcgccaa ccgccttctg cgcgttggcg cggatctcac cgggatgggc cgcgcgctgg
     1081 ccgaggtgca cgccttcgct cgcgggcacg cgggcggttt cgatcccgag ctcgcggcca
     1141 aggctttcgc cgtcgtcgcc gcgtggtcgc gcgtggatgg acggatcgtc gcacccaccc
     1201 ggctgcaaac ggcgatcgag caagcggagc gatacctggt cgagcaaagc cacgctccga
     1261 tcgacctgca tcaactcgcc cagtcgctcg gcgtcgccta ttcccatttc cgtcgcgctt
     1321 ttcgaaagaa gaccggctat gcgccgtggc agtat
//
LOCUS       DASPLT010000036         2485 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_618, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000036 DASPLT010000000
VERSION     DASPLT010000036.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 2485)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2485)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2485
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_618"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..177)
                     /locus_tag="VEA63_00410"
     CDS             complement(<1..177)
                     /locus_tag="VEA63_00410"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HYD82467.1"
                     /translation="MIAVRELSKRFGSREVLRGLDLDVTPGVVTLLVGANGAGKTTLL
                     RAITGLCTPDRGSVQ"
     gene            complement(174..1469)
                     /locus_tag="VEA63_00415"
     CDS             complement(174..1469)
                     /locus_tag="VEA63_00415"
                     /inference="COORDINATES: protein motif:HMM:NF016906.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NosD domain-containing protein"
                     /protein_id="HYD82468.1"
                     /translation="MTPRSQTARWSWILLPLFVASVVHAATDPHWLQHEIENATAGAT
                     IDVPAGVHAGPFVIRQTLHLRGAPGATLEGDGRGHVVEVLAPDVEISGFVIRRSGSNL
                     SNDHAGVHVSAPRVFLHHNTIVDTLHGIYLRQAPEASLTHNIIRGRAAVVAIADPLTS
                     RLQLSSDELCSVTLEQDQRGNGIHIWNSRGHTIAENDIRGTRDGIYFSFCDDTTVRRN
                     TIQDVRYGLHYMYSDNNVFEENLFTHNAAGSALMYSSGIVLRQNRFVANRSHRAYGLL
                     LHTVENTRIENNLVEGNTVGVFIENSAANVVVGNTVASNYIGVRLSDSSGEHRFSANI
                     LRGNIHAVETSGLDSTNRWSIDGVGNFWDDSAKLDLDRNGIADVPHFEADLFGPWRRS
                     VPEIGLLSASPGERALRFVHARLRLPGAPGVTDRSPLTR"
     gene            complement(1466..2083)
                     /locus_tag="VEA63_00420"
     CDS             complement(1466..2083)
                     /locus_tag="VEA63_00420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD82469.1"
                     /translation="MGNTTRLIYRFLRRPPSATARVLLVLAAASLVAALFLPLWEIRL
                     VAPQYQEGLSLHIYSYKLIAGNNGQDLVEINNLNHYIGMKPLEEADFIEMRWVPFALG
                     VFILLALRSSAIGIMRGVVDLLVLFLYFTAFSFGSFYYRLYTYGHQLDPTAPMTIKPF
                     TPVLIGTQQIANFVQSSFPDWGTLFLGLFPVLVVGAMWFSRKEET"
     gene            complement(2113..>2485)
                     /locus_tag="VEA63_00425"
     CDS             complement(2113..>2485)
                     /locus_tag="VEA63_00425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HYD82470.1"
                     /translation="ENTNPHAVWEAGQTGVTRNGTTVEAKVMAIRSRFIPDKIEVNEG
                     DTVVLHLTNVEQTTDMIHGWGLIEHDLNVIVDPGETKTVRFTASKPGVFAFYCTNFCS
                     ALHQEMQGYLAVRPAAQTAQR"
BASE COUNT          456 a          666 c          905 g          458 t
ORIGIN      
        1 ttggacgctg ccgcggtcgg gggtgcagag gccggtgatc gcgcgtagca aagtggtttt
       61 gccggcgccg ttcgcgccca cgaggagcgt cacgacaccg ggtgtcacgt cgaggtcgag
      121 tccgcgcaac acctcgcgcg agccgaagcg tttcgagagc tcgcgaacgg cgatcatcgt
      181 gtgagcgggc tgcgatcggt gacgccgggc gcgccgggca agcggagacg tgcgtgcacg
      241 aagcgcagcg cgcgttcgcc ggggctggcg gagagcaggc cgatctcggg aacactgcgg
      301 cgccacgggc cgaagaggtc ggcttcgaaa tgcggcacgt cggcgatgcc gttgcgatcg
      361 agatcgagct tggcggagtc gtcccagaaa ttgccaacac cgtcgatcga ccagcggttg
      421 gtcgagtcga gtccgctggt ttcgaccgcg tgaatgtttc cgcgcaggat gttcgcggag
      481 aagcggtgtt cgccggagct gtcggagagg cggacgccga tgtagttcga ggcgacggtg
      541 tttccgacaa cgacattggc cgcggagttt tcgatgaaga cgccgacggt gttgccttcg
      601 acgaggttgt tttcgatgcg ggtgttttcg acggtgtgca gcagcaggcc atacgcgcgg
      661 tggctgcggt tggcgacgaa gcggttttgg cggagcacga tgccgctcga atacatgagg
      721 gcggagccgg cggcgttgtg cgtgaagagg ttttcttcaa acacattgtt gtccgaatac
      781 atgtagtgca gcccgtagcg gacatcctgg atcgtgttgc ggcgaacggt cgtgtcgtcg
      841 cagaaggaaa aataaattcc gtcgcgggtg ccgcggatgt cgttctcggc gatggtgtgg
      901 ccgcggctgt tccagatgtg aatgccgttg ccgcgctggt cctgctcgag ggtgacggag
      961 cagagttcgt cgctcgagag ctgcagtctg ctcgtgagtg gatcggcgat cgcaacgacc
     1021 gcggcgcggc cgcggatgat gttgtgcgtc aaactcgctt ccggcgcctg gcgcagatag
     1081 atgccgtgaa gcgtgtcgac gatggtgttg tgatggagaa acacgcgtgg agcgctcacg
     1141 tgaacgcccg cgtggtcgtt ggagaggttc gagccggaac gacgaatgac gaagccggaa
     1201 atctcgacgt caggcgcgag cacttcgacc acgtgaccgc ggccgtcgcc ctcgagcgtg
     1261 gcgccgggcg cgccgcgcag atggagcgtt tggcggatta cgaacggtcc tgcgtgcacg
     1321 ccggcgggga cgtcgatcgt tgcaccggcg gtggcatttt cgatctcatg ttgcagccag
     1381 tgagggtcgg ttgcggcgtg aaccaccgac gcaacaaaca ggggaagaag aatccacgac
     1441 catcgcgcgg tctgcgaacg tggcgtcatg tttcctcctt tcgcgaaaac cacatcgcgc
     1501 cgaccaccaa cacggggaac aggccgagaa agagcgtccc ccaatcggga aagctgctct
     1561 gcacgaagtt cgcgatttgc tgcgtgccga tcagcacggg agtgaacggc ttgatcgtca
     1621 tgggcgcggt cggatcgagc tgatgcccgt aagtatagag ccggtaatag aagctgccga
     1681 aggagaacgc ggtgaaataa aggaagagca cgagcagatc gacgacaccg cgcatgatgc
     1741 cgatggccga ggagcggagg gcgagcagga tgaacacgcc gagggcgaat ggcacccacc
     1801 gcatctcgat gaagtcggcc tcctcgagcg gcttcatgcc gatgtagtga ttgaggttgt
     1861 tgatctcgac cagatcctgg ccgttgtttc cggcgatgag cttgtagctg tagatatgga
     1921 gcgagaggcc ttcctgatat tgcggagcga cgagacggat ttcccacaaa ggcaggaaga
     1981 gcgcggcgac gagcgaggcc gcggcgagca cgagcagcac gcgtgcggtc gcactcggcg
     2041 ggcggcggag gaaccggtag atgagccgcg tggtgtttcc catcgcgagt gcggcggcgc
     2101 ggtggggccg cgtcagcgtt gagcggtttg tgcggcgggc ctcacggcga gataaccctg
     2161 catttcctgg tgcagcgcgg agcagaagtt cgtgcagtaa aaagcgaaca cgccgggctt
     2221 cgacgcggtg aagcgcaccg ttttcgtttc gccggggtcg acgatgacgt tgaggtcgtg
     2281 ctcgatcaga ccccagccgt gaatcatgtc ggtggtctgc tcgacgttgg tgaggtggag
     2341 cacgacggtg tcgccttcgt tgacctcgat tttgtcggga atgaagcggc tgcgaatcgc
     2401 catcaccttg gcttcgacgg tggtgccgtt gcgcgtgacg ccggtctggc cggcttccca
     2461 cacggcgtga ggattggtgt tctcg
//
LOCUS       DASPLT010000037         1226 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_61931, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000037 DASPLT010000000
VERSION     DASPLT010000037.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1226)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1226)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1226
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_61931"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1122)
                     /locus_tag="VEA63_00430"
     CDS             complement(<1..1122)
                     /locus_tag="VEA63_00430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD82471.1"
                     /translation="MARRAPPIAVRVYTFGCTHDVEDCTPPDRRHAQPDNRMPRLAVL
                     LAAAACALAAQSVASSPSTTLGEQGHAPTRVLAFDELPGEPELNHMAFGPDGLLFATS
                     RGGFYTNDGASWRFHPSINAHDSLPSLHIDRAGRVFTARGKEPLMAVPQADGSYRWQS
                     LASPTLPPEENLSWVHCIHAGEGAWFQTLGKSSRGYALWHWHEGTWRKIHANATEPYL
                     SGPAGVFVRNPAGRLQRLAPGTPEEIGPAPTEPIRSISSPIGDTGIWFVATASELHRV
                     TRAACTVWPNEAADYLRAHKIARIFSRPDGSLVVATNDGAIVQLDAHGRWRGLSSAEN
                     GLPEATFLNATFDDADGLWNVYSNQLARVDLAASHTVFDA"
BASE COUNT          199 a          342 c          473 g          212 t
ORIGIN      
        1 cgcgtcgaac accgtgtgcg acgcagcgag atcgacgcgc gcgagctggt tggaatacac
       61 gttccacagt ccgtcggcgt cgtcgaaggt ggcgttcaag aacgtggcct cgggcaaacc
      121 gttttcggcg ctgctcaggc cccgccagcg tccgtgcgcg tcgagttgga cgatcgcgcc
      181 gtcgttggtg gcgacgacga gcgaaccgtc ggggcgggaa aaaatgcgag cgatcttatg
      241 agcgcgcagg tagtcagcgg cttcgtttgg ccaaacggtg caagcggcgc gcgtgacacg
      301 gtggagctcc gacgcggtcg caacgaacca gatgccggta tcgccgatcg gagacgaaat
      361 gctgcggatg ggctcggttg gcgcgggacc gatttcctcg ggagtgccgg gcgcgaggcg
      421 ttggaggcgg cctgccgggt tgcgcacgaa gacgccggcg gggccgctga gatagggttc
      481 ggtggcgttg gcgtggattt ttcgccacgt gccttcgtgc cagtgccaga gggcgtagcc
      541 gcgcgacgat ttgcccaggg tttgaaacca cgcgccctcg cccgcgtgga tgcaatggac
      601 ccaagagagg ttttcttctg gcggcagggt gggcgaggcg agggattgcc agcggtaact
      661 gccgtcggct tgagggacgg ccatgagcgg ctcttttccg cgagcggtga aaacgcggcc
      721 ggctcgatcg atgtgcaatg acggcaggct gtcgtgggcg ttgatgctcg gatgaaaacg
      781 ccaacttgcg ccatcgttcg tgtagaagcc gccgcgcgac gtggcgaaga gcaatccgtc
      841 ggggccgaac gccatgtggt tcagttcggg ttcgccgggg agttcgtcga aggcgagcac
      901 gcgcgtgggg gcgtgcccct gttcgccgag cgtggttgag ggagacgatg cgacgctttg
      961 cgccgcgagt gcgcaggcgg cggcggcgag caggacggcg aggcgcggca tgcgattgtc
     1021 gggctgagcg tggcggcggt cgggcggcgt gcaatcctcc acgtcgtgcg tgcacccaaa
     1081 ggtgtagacg cgaacggcga tcggcggtgc gcgacgcgcc atgcacccaa gggtgtatgg
     1141 cgaggttttt gccaactggt aatctcaacg acgcagccgg tcggtctttt gctgagcgcg
     1201 atcgtgaggc ggaccggcgc ttccac
//
LOCUS       DASPLT010000038         2127 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_102978, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000038 DASPLT010000000
VERSION     DASPLT010000038.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 2127)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2127)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2127
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_102978"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..245
                     /locus_tag="VEA63_00435"
     CDS             <1..245
                     /locus_tag="VEA63_00435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD82472.1"
                     /translation="GDVNAFDSASPVIVFGAVDTVNITGGNLEQPNGRTINVSGIGEL
                     KFLDGMTSQGVTLPAQNNEAELTEEGVNVTDLIVKP"
     gene            462..1124
                     /locus_tag="VEA63_00440"
     CDS             462..1124
                     /locus_tag="VEA63_00440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012377048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HYD82473.1"
                     /translation="MRIVIVEDHLMFRDVIRKICTTQFGHEVVGETDSGVKAVELILE
                     LSPDVVILDLSLPDMDGFNVVDRVFAAMPGLRILVLSSHCDDYTLFRVEKSGVHGFID
                     KNSNTVETLQDALSSIAAGRIYFSAAFQSAKLARRTDPRSFTKVLSEWERAILSLIGQ
                     GLSDEEIGARLNLSHRTVQTHRSNILRKLDIKGTPKLIAFAIEHGFTQVPSKHGSNPV
                     FS"
     gene            complement(1078..>2127)
                     /locus_tag="VEA63_00445"
     CDS             complement(1078..>2127)
                     /locus_tag="VEA63_00445"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HYD82474.1"
                     /translation="VRLFVRDQGPGMSDAEAAALFTKFTRLPQAHANNIRGSGLGLAV
                     CRLLATRMGGAVGVDSRLGEGSTFWADLPLPAAANAPATPVLDAPSATPLRALIVEDI
                     DYNATAMQAVLRKLGIVSEVASDGITAFEKLQATFYDVAFMDWNLPGMIGTEVVARYR
                     AVEPPNRRTIIVATTAYSGDVNREACLQAGMDAFIAKPFTPEKIGAALADLRGSLRAA
                     ASVSVRSPGTAAPVPTAEFDLQMLRFLADETPDGLRTQIDRYVASFDADREAAHGIVA
                     AGDAKEIHRIAHRLVGHASAIKYEPLVRLARELQAHAATDDPARLRRLLGEFDAEFRA
                     LRKKLDSTRVSTAPA"
BASE COUNT          393 a          629 c          712 g          393 t
ORIGIN      
        1 tcggcgacgt caacgcgttc gactcggcaa gcccggtgat tgttttcggc gcggtcgaca
       61 ccgtgaatat caccggcggc aatctcgagc agcccaacgg tcggacgatc aatgtcagcg
      121 gcatcggaga gttgaagttc ctcgatggca tgacgtcgca aggcgttacg ctgcccgcgc
      181 agaacaacga agccgagctc accgaagaag gcgtcaacgt caccgacctt atcgttaagc
      241 cgtaatgtcg cgagacggcc ttggaggccg gcgatcattt cagcccgcgg tgcgaaccgc
      301 gggctttttt attgaaggac cgcgagatcc gcggcggcgt gaaagtgaaa gcacgaagtt
      361 tgtcagcatc ggtgcggggg cggcgagcag gtcggcgctt ctttccggaa tacatcgcag
      421 caggcttgag cctgaagggc atccccgttg catttctctc tgtgagaatt gttatcgtcg
      481 aagatcacct catgtttcgc gatgtgatcc ggaagatctg caccacgcag ttcggtcacg
      541 aggtcgtggg tgagaccgat tccggggtga aggcggtcga actgattttg gagctgagcc
      601 ccgacgtggt gattctggat ttgtcgctgc cggacatgga cggcttcaac gtcgtggatc
      661 gcgttttcgc ggcgatgccc ggcttgcgaa tcctcgtcct ctcttcgcat tgcgatgatt
      721 acacgttgtt tcgcgtcgag aagtccggcg tccacgggtt catcgacaag aactcgaaca
      781 ccgtcgaaac gctgcaggac gcgttgtcgt cgattgccgc cgggcggatt tatttctccg
      841 ccgcgttcca gtcggcgaag ctcgcgcggc ggaccgaccc gcgatcgttc acgaaagtgc
      901 tttccgaatg ggagcgcgcg atcctgtctt tgatcggcca gggcttgagc gacgaggaga
      961 tcggcgcgcg actaaatctc tcccaccgca ccgtgcagac gcaccgcagc aacattttgc
     1021 ggaaactcga tatcaagggc acgccgaagc tgatcgcctt cgcgatcgag cacggcttta
     1081 cgcaggtgcc gtcgaaacac gggtcgaatc cagttttttc ctgagggccc taaactcggc
     1141 gtcgaactcg ccgagcagcc gccggagccg cgcgggatcg tcggtggcgg cgtgcgcctg
     1201 gagttcgcgc gcaaggcgca cgagcggctc gtatttgatc gcactggcgt gaccgaccag
     1261 acggtgggcg atgcggtgaa tctccttcgc gtcgcccgcg gcgacaatcc cgtgcgcggc
     1321 ctcgcggtcg gcgtcaaacg acgcgacgta acggtcgatt tgcgtgcgca ggccgtcggg
     1381 cgtttcgtcg gcgaggaaac gcagcatttg caaatcgaac tccgccgtcg gaaccggcgc
     1441 ggcggtgccg ggcgacctca cggaaaccga cgccgcggcg cggagcgaac cgcgaaggtc
     1501 ggcgagcgcg gcgccgattt tttccggggt gaacggcttt gcgataaacg catccatgcc
     1561 agcttgcagg cacgcctcgc gattgacgtc gccggagtaa gcggtcgtgg cgacgatgat
     1621 cgtgcgtcgg ttcggcggct cgacggcgcg ataacgcgcg acgacttcgg tgccgatcat
     1681 cccgggaaga ttccaatcca tgaacgcgac gtcgtagaac gtggcctgta atttttcgaa
     1741 agcggtgatg ccgtcggaag cgacttcgga aacgatgccg agtttgcgca acacggcctg
     1801 catcgcggtc gcgttgtaat cgatgtcctc gacgattagc gcgcgcagcg gcgtggcgga
     1861 aggcgcgtcg agaacgggcg tcgccggtgc gttcgcagcg gcgggcagcg gaagatccgc
     1921 ccagaacgtc gagccttcgc ccagacgcga atcgacgccg acggcgccgc ccatgcgcgt
     1981 ggcgagcagg cggcacacgg cgaggccgag acccgagccg cggatgttgt tcgcatgggc
     2041 ttggggcaga cgcgtgaact tggtgaagag tgcagcggct tccgcgtcgc tcatgccggg
     2101 accctggtcg cgcacgaaga gacgcac
//
LOCUS       DASPLT010000039         2504 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_225789, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000039 DASPLT010000000
VERSION     DASPLT010000039.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 2504)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2504)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2504
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_225789"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(51..494)
                     /gene="nusB"
                     /locus_tag="VEA63_00450"
     CDS             complement(51..494)
                     /gene="nusB"
                     /locus_tag="VEA63_00450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HYD82475.1"
                     /translation="MSKALFAQRRDGRVAALQYLFAWSMNAPKNLAEDLRTFFENMEQ
                     PRDHYAFGEEVIHGVIDNIADIDARIKGLAHNWEFERIAKIDLTILRIAIFEMIHRKD
                     IPPVVSINEAIDLSKQFSNADAKRFINGILDRLKDQVGRDARKAE"
     gene            complement(491..964)
                     /gene="ribH"
                     /locus_tag="VEA63_00455"
     CDS             complement(491..964)
                     /gene="ribH"
                     /locus_tag="VEA63_00455"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009508958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HYD82476.1"
                     /translation="MSLAAPSAVVVNGAAFRVGIVAARFNADLVDALLARVIETLRAA
                     GVKEKRISIARVPGSHEVPWAAAELARRSRNDVVIGLGVLIGGDTSHHEMVGQSVSAA
                     LQRVALDTRTPVINGVIVANTRAQAEARCIGKINRGAEFAAAALEMAALKKILSR"
     gene            complement(1208..2311)
                     /gene="ribB"
                     /locus_tag="VEA63_00460"
     CDS             complement(1208..2311)
                     /gene="ribB"
                     /locus_tag="VEA63_00460"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HYD82477.1"
                     /translation="MSASVHDSFDSVESAIAEIAAGRLIIVTDDEDRENEGDLIMAAA
                     KATPETVNMMIRYCSGIVCVPTFEPQLRRLGLGPMVQQNREVQRTDFTVSVDAADGIT
                     TGISAHDRTQTIRILADPNARAEQLVQPGHVFPLRAKSGGVLERAGHTEAAVDLAQLA
                     GLHPSGVLCELINDDGSVQRLPQLIEFKQRFGLRMISIAQLIEYRAKRDNLIELVCTR
                     PFPSDFGEFTLHVFRSKLDNRHHLALAMGSLTAEPTLVRVHSENLLGDVFRMRGVGTH
                     QTLTASLEAVAREGRGVVLYMEHANGGAELIRRLDAKPGEAPPPMSFRDYGIGAQILA
                     ALGLRKIRLLSKSARRVVGLDGYGLEIVEQVPL"
BASE COUNT          500 a          914 c          649 g          441 t
ORIGIN      
        1 atgacaaacg aacaattcgc gacgattcgc gtgattcgcg ggcaacctga tcactctgcc
       61 ttccgcgcat cccggccgac ctgatctttc aaccggtcga gaatgccgtt gatgaaccgc
      121 ttcgcgtcgg cgttggaaaa ctgcttcgac aggtcgatcg cctcgttgat cgacaccacc
      181 ggcggaatgt ccttgcgatg aatcatctcg aaaatcgcga tccgcaaaat cgtcagatcg
      241 atcttcgcga tccgctcgaa ctcccagttg tgcgcgagcc ccttgatgcg tgcatcgatg
      301 tccgcgatgt tgtcgatcac cccgtgaatc acttcctcgc cgaacgcgta gtgatcgcgc
      361 ggctgctcca tgttctcgaa gaacgtccgc agatcctccg cgaggttttt cggagcgttc
      421 atgctccagg caaacaggta ttgcagcgcg gcgacgcgac catcgcggcg ttgcgcaaat
      481 agggctttgc tcatcgggaa agtatcttct tcaacgccgc catctccagc gccgcggcgg
      541 cgaattcggc gccgcgattg attttgccga tgcagcgcgc ctcggcctgc gcacgggtgt
      601 tggcgacgat cacgccgttg atgaccggcg ttcgcgtgtc gagtgccacg cgttgcagcg
      661 ccgccgatac gctttgcccc accatttcgt gatgcgacgt atcgccgccg atcaacacgc
      721 ccagcccgat cacgacatcg ttccgcgacc gccgcgccag ctccgccgcc gcccacggca
      781 cctcgtgcga acccggcacc cgcgcgatcg agatccgctt ttccttcacc cccgccgcac
      841 gcaacgtctc gatcactctg gccaacaacg catcgacgag atccgcgtta aaccgcgccg
      901 ccacaatgcc gacgcgaaac gcggcaccgt tgacgacaac tgcactggga gcggccagac
      961 tcatgaaccg gctatcgact aggccaaagc cacattccgc aactcaaatc ctgttcgacc
     1021 caaatgcgaa ggagccacga atggacacga ttttacacga atagagatcc aaggcatcga
     1081 acaacgatgc attccattcg tgtgaattcg tgtccattcg tggttcacaa cccggattga
     1141 aatccgcgtt catccgtgtc cacttgtgcg cctctggagc atccgtggtt aagctaaaac
     1201 caccgcgtta caacggcacc tgctccacga tctccagccc atagccatcg agcccgacca
     1261 cccgccgcgc gctcttcgac aacaagcgga tcttccgcaa acccaatgcc gccaaaatct
     1321 gcgcgccgat gccgtaatca cgaaagctca tcggcggcgg cgcctcgccc ggcttcgcat
     1381 cgagccgccg gatcaactcc gcgccgccgt tcgcatgctc catgtaaagc accacgccac
     1441 gcccctcgcg cgcgaccgct tcgagcgacg ccgtgagcgt ctgatgcgtg cccacaccgc
     1501 gcatccgaaa cacatcgccc aacaaattct cgctgtgcac ccgcacaagc gtcggctccg
     1561 ccgtcaacga acccatcgcc agcgcgagat gatggcgatt gtccaacttg ctgcgaaaca
     1621 catgcagcgt gaactcgccg aagtccgacg gaaacggccg cgtgcagacc agctcgatca
     1681 gattgtcgcg cttcgcccga tactcgatca gctgcgcgat cgaaatcatc cgcagcccaa
     1741 accgctgctt gaactcgatc aactgcggca gccgctgcac cgagccgtca tcgttgatca
     1801 actcgcacaa cacgccactc gggtgcagcc ccgcgagctg cgccagatcg accgccgcct
     1861 ccgtgtgacc cgcccgctcg agcacgccgc cggatttcgc gcgcaacgga aacacatgcc
     1921 ccggctgcac caactgctcc gcgcgcgcgt tcggatccgc gagaatgcga atcgtctgcg
     1981 tgcgatcgtg cgcgctgatg cccgtcgtga tcccatccgc cgcgtcgacg ctcaccgtga
     2041 aatccgtccg ctgcacctcg cgattctgtt gcaccatcgg cccgagcccg agccggcgca
     2101 gttgcggctc gaacgtcggc acgcacacga ttccgctgca gtagcgaatc atcatgttca
     2161 ccgtttccgg cgtggccttc gccgccgcca tgatgagatc gccctcgttc tcgcgatcct
     2221 cgtcatcggt aacgatgatc agccggcccg ccgcgatttc ggcaatggcc gactccacgg
     2281 agtcgaacga atcgtgcacg gaagcggaca tcgccgaaaa atcgccagcg ccctccagcg
     2341 tgtcaaccgc cgcgcagccc cgcctccgat gtagggcgag gtctcccgac cccgccgaac
     2401 gcacgccctt cccaaccgtc acctgtcgaa caagcccgac gaagatgtcg gactccaccc
     2461 agcctcactc tcacgtccac tcagcagccc gcccgcgaca ccac
//
LOCUS       DASPLT010000040         4567 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_184950, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000040 DASPLT010000000
VERSION     DASPLT010000040.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 4567)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4567)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4567
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_184950"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..564)
                     /locus_tag="VEA63_00465"
     CDS             complement(<1..564)
                     /locus_tag="VEA63_00465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD82478.1"
                     /translation="MIDVRPSSRAVLEILDTRPAVDVSQAEYWRLLGYPRDHEPSERA
                     RELAAWARQWYAEHGRPWVYLREAQLHVGPDTLKFDGAEFHSPRLRELFRDAGVQRAM
                     FVAVSAGRACEEHARRLWQESKPDEYFFLEIFGSAVVEQLCATLSGRICDFADRDELM
                     AVPHYSPGYTGWDIADQNKLFELIARGV"
     gene            complement(561..2216)
                     /locus_tag="VEA63_00470"
     CDS             complement(561..2216)
                     /locus_tag="VEA63_00470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012377139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASKHA domain-containing protein"
                     /protein_id="HYD82479.1"
                     /translation="MSVKLHFNSETVDATPGHSLFTHAEELGIPVPTSCNKNGKCKEC
                     VVEVTEGMECLSKPGPEEKHLKGAFRLSCCARITQEDGTVRCHTMRRGTMRIEKHAFE
                     LPVHGPKWTLDPAVKRDGDRILLDGEEIDRSTGPIHGLAIDLGTTTVVMRLLNLETGE
                     VVADSSFENPQRFGGSDVMARIQYDTDDRTKTLQRTLVGYLGRAIKEFPVDSRSIYEV
                     VIAGNSTMRDMFFKLSVYSIGQSPYQSITELELADGKRTTTSLAEPARKLGLPIHPKA
                     RVYGMPIISGHVGADAAACMLAIDIANEERVVALMDIGTNTELIVGNKHRVLAASCPA
                     GPAFEGGQITFGMPGLPGAIEKVKIDDDGAVQATVIGDVKPEGICGSGLVDLMSELLR
                     TERMNLLGRFEDSGTDFVVAMNDDQPITFNEADINALAQAKGANVAGLQIAFAEYGIS
                     YKDLDVFYLAGGFGRHLNLESSKRIGLIPSIPAEKIVQVGNASVEGACIALLSRAKRA
                     ELEHLVRNITHCRLETHPGFFDFFVNGCQFAPVGDSVEEVSGS"
     gene            complement(2357..3427)
                     /locus_tag="VEA63_00475"
     CDS             complement(2357..3427)
                     /locus_tag="VEA63_00475"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012377140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HYD82480.1"
                     /translation="MKLGLGLYRHMLTRENFQFARQAGATHIVAHLVDYFRGSTHQTR
                     DDQPTGTDRGWGIAGDPNQLWTLEELVSLRRNVEAEGLKLEAIENFDPAHWHDILLDG
                     PKRAQHIENVKTILRRMGEAGIPVMGYNFSIAGVAGRTTGPYARGGAPSVGMEGTYDL
                     PMPNGMVWNMIYDEHAPEGFLPSITHEELWRRLDAFLQEVLPVAEAAGVKLAAHPDDP
                     PTPTVRAQPRLVYQPRHYQKLLDLNPSPANSLEFCIGSLAEMTEGDIYETVDTYSRQG
                     RLGYVHFRNITGKVPTYKETFIDDGDVDMLRVLEILKRNKFDGVLIPDHTPAMTCAAP
                     WHAGMAFAMGYMKAAMRAVGVG"
     gene            complement(3479..4519)
                     /locus_tag="VEA63_00480"
     CDS             complement(3479..4519)
                     /locus_tag="VEA63_00480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012377141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HYD82481.1"
                     /translation="MNLRQLARLAQLSPSAVSLALRDSSKISEATKKKVRQIAEKAGY
                     RPDARVVAMMTHLRKPRAVRQQACFGVISFYPARHPWENSRHLAQVYEGMQRRASELG
                     YRLEPLWLREPGMTHRRFRSILDTRGIEGLLCFGSPDLEQEFPAELGRCAIVTVGLSI
                     RTPLHRVTSHFYNDTINVLNRVHQLGYRRPGLVLGRYEAARSGHAHTAAYLGWCEHML
                     GPGRALPVQLVDRVEEKPLMEWLEGQRPDVLIFAHLYDMLPELRALLKKNRVRVPAQL
                     GVAVLSQILDGSGFSGLQQNQPLMGAWAVELLAARIANHDLGIPANPRTEMVESRWIE
                     NRSLRSRPQVNS"
BASE COUNT          858 a         1547 c         1313 g          849 t
ORIGIN      
        1 cacgccgcgc gcgatcaact cgaaaagttt gttctggtcc gcgatgtccc agcccgtgta
       61 acccggacta taatgcggca ccgccatcaa ctcgtcgcgg tccgcgaaat cgcagatgcg
      121 tccgctcaac gtcgcacaca actgctccac caccgccgag ccgaagatct ccaggaaaaa
      181 gtattcgtcc ggtttcgact cctgccagag ccggcgcgcg tgttcctcgc aagcccgacc
      241 cgcactcacc gccacaaaca tcgctcgctg cacgcccgca tcgcggaata gttcgcgcag
      301 gcgcggcgaa tgaaactccg cgccgtcgaa cttcagcgta tccggaccga cgtgaagctg
      361 cgcctcgcgc aaataaaccc acggccggcc gtgctccgca taccattgcc gcgcccacgc
      421 cgcgagttca cgcgcgcgtt cgctcggctc gtgatcgcgc ggataaccga gcagccgcca
      481 atactccgcc tgcgacacgt cgaccgccgg tcgcgtatcc aaaatttcca gcacagcacg
      541 ggagctcggg cgcacgtcga tcatgagccg gaaacctcct ccaccgaatc gcccacgggg
      601 gcgaactggc agccgttgac gaagaaatcg aaaaacccgg gatgcgtctc gagccggcaa
      661 tgcgtgatgt tgcgcacgag gtgctcgagc tccgcgcgtt tcgcgcgcga gagcagcgcg
      721 atgcacgcac cttcgaccga cgcgttgccc acctgcacga ttttctccgc aggaatgctt
      781 gggatcagcc caatgcgctt cgacgactcg aggttcaggt gccggccaaa tccgcccgcc
      841 agataaaaca catcgaggtc cttgtagctg atgccgtatt cggcgaacgc gatttgaaga
      901 ccggcgacgt tcgcgccttt cgcctgcgcc agcgcattga tgtcggcttc gttgaacgtg
      961 atcggctgat cgtcgttcat cgcgacaaca aaatccgtgc cgctgtcctc gaagcgcccg
     1021 agcaaattca tccgctccgt gcgcagcagc tcgctcatca agtcgacgag gccggaaccg
     1081 caaatcccct ctggtttcac gtcgccgatc accgtcgcct gcaccgcgcc atcgtcgtcg
     1141 atcttcacct tctcgatcgc gcccggcagc cccggcatgc cgaacgtgat ttgtccgcct
     1201 tcgaacgccg gccccgccgg acacgacgcg gcaagcaccc ggtgtttatt gcccacgatc
     1261 agctccgtgt tcgtgccgat gtccatcaac gccacgaccc gctcttcgtt cgcgatgtcg
     1321 atcgcgagca tgcaagcggc ggcatccgcg ccgacatgcc cgctgatgat cggcatgcca
     1381 tacacccgcg ctttcggatg aatgggcagg ccgagcttgc gcgcgggctc cgcgaggctc
     1441 gtcgtcgtgc gctttccgtc cgcgagttcg agttcggtga tggactgata cggactctgc
     1501 ccgatcgagt agaccgacag tttgaaaaac atgtcgcgca tcgtcgaatt gcccgcgatc
     1561 acgacttcgt agattgaacg cgagtcgaca ggaaactcct tgatcgcccg gccgagataa
     1621 ccgacgagcg tccgctgcaa agtcttcgtg cgatcgtccg tgtcgtattg gatccgcgcc
     1681 atcacgtccg agccgccgaa acgttgcgga ttttcgaacg acgaatccgc cacgacttcg
     1741 cccgtctcca aattcaggag acgcatcacc accgtggtcg tgccgagatc gatcgcgagc
     1801 ccgtgaatcg gccccgtgct gcgatcgatc tcctcgccat cgagcagaat gcgatcgccg
     1861 tcgcgtttca ccgcgggatc gagcgtccac ttcggcccat gcacgggcaa ttcgaacgcg
     1921 tgtttctcga tgcgcatcgt gccgcggcgc atcgtgtgac agcgcaccgt gccgtcctcc
     1981 tgtgtaatcc gcgcgcaaca cgaaagacgg aacgcgccct tcaagtgctt ctcctcgggg
     2041 ccgggtttcg agaggcactc catgccctcg gtcacctcga cgacgcactc cttgcacttg
     2101 ccatttttat tgcaagaggt cgggacggga atgccgagtt cctccgcatg cgtgaagagc
     2161 gaatgccccg gcgtggcatc gacggtctcg ctgttgaaat gcagcttcac gctcatgacg
     2221 cgctccgtag cggcaggcgt ccctgcctgc ctgcctttgt ttgtgcgcac gcgattcgag
     2281 caggcaggca cggaggcccg ccgctacggc agacggactc gctcagcgca atttgcctca
     2341 acgcgttcat actcactcaa cccactccca ccgcgcgcat cgccgccttc atgtaaccca
     2401 tcgcaaacgc catgcccgca tgccacggcg cggcgcacgt catcgccggc gtgtgatccg
     2461 ggatgagcac gccgtcgaat ttgttgcgtt tcaagatctc caacacgcgc aacatgtcga
     2521 cgtcgccatc gtcgatgaac gtttctttgt aagtcggcac cttgccggtg atgttgcgga
     2581 aatgcacata gccgagccgc ccttggcggc tgtaggtgtc cacagtttcg tagatgtcgc
     2641 cctccgtcat ttccgcgagc gaaccgatgc aaaactcgag gctgttcgcc ggactcggat
     2701 tcagatcgag caacttctgg taatgacgcg gctgatacac caatcgcggt tgcgcgcgca
     2761 ccgtcggcgt cggcggatcg tccggatgcg cggcgagttt cacgcccgcc gcttcggcga
     2821 cgggcaacac ttcctgcaga aacgcgtcga ggcgcctcca caattcttcg tgcgtgatcg
     2881 acggcagaaa gccctccggc gcgtgctcgt cgtaaatcat gttccacacc atcccgttcg
     2941 gcatcggcag atcgtaggtg ccttccatgc cgaccgacgg cgcaccgccg cgcgcatacg
     3001 gacccgtcgt gcggccggcg acgcctgcga tgctgaaatt gtagcccatc accggaatgc
     3061 ccgcctcgcc catccggcgc aggatcgtct tcacgttctc gatgtgctgc gcgcgtttcg
     3121 gcccatcgag caaaatatcg tgccagtgcg cgggatcgaa attttcgatc gcctcgagct
     3181 tcaaaccctc cgcttcgaca ttgcgccgca gcgacacgag ttcctcgagc gtccaaagtt
     3241 gatttggatc gcccgcgatg ccccagccgc gatcggtgcc cgtcggttga tcgtcacgcg
     3301 tctggtgcgt gctgccgcga aaataatcca ccaagtgcgc gacgatgtgc gtcgcacccg
     3361 cctgccgcgc gaactgaaag ttctcacggg tcaacatatg gcgataaagg ccgaggccta
     3421 gtttcatggg gaaggaccaa ggtgtgtgcg tccgcgcgtt tgacgaacgc aaacgcactc
     3481 aactgttgac ctgcggccgg ctccgcagcg agcggttctc gatccagcgg ctctcgacca
     3541 tctcggtcct cggattcgcg gggataccca agtcgtgatt cgcgatgcgc gcggcgagca
     3601 gttccaccgc ccacgcgccc atcaacggct ggttttgttg cagcccggaa aaccccgagc
     3661 cgtcgagaat ctggctgagc acggccacac cgagctgcgc cggcacgcga acgcgatttt
     3721 ttttcaacaa cgcgcgcagc tccggcaaca tgtcgtagag gtgcgcgaaa atcagcacgt
     3781 ccggccgctg gccttcgagc cattccatca agggcttttc ttccacgcga tcgaccaatt
     3841 gcacgggcaa tgcgcgaccg ggacccagca tgtgctcgca ccaacccaga tacgcggcgg
     3901 tgtgcgcgtg cccgctgcgc gcggcttcgt aacgaccgag cacgagccct ggccggcgat
     3961 aaccgagctg atgcacgcgg ttaagcacgt tgatcgtgtc gttataaaag tggctcgtaa
     4021 cgcgatggag cggcgtgcgg atgctcaggc caaccgtcac gatcgcgcag cgaccgagtt
     4081 cggcgggaaa ctcctgctcc agatccgggc ttccgaaaca cagcaggccc tcgatcccgc
     4141 gcgtgtcgag aatcgagcga aaccgccggt gcgtcattcc aggctcgcgc aaccacaacg
     4201 gctccagccg gtagccgagc tcgctcgccc gccgctgcat gccttcgtaa acctgcgcga
     4261 gatggcgcga attttcccat gggtgacgcg ccggatagaa tgagatcacg ccgaagcacg
     4321 cttgctggcg caccgcgcgc ggcttgcgca gatgcgtcat catggcgaca acgcgcgcgt
     4381 ccggacggta gcccgctttc tccgcaatct gccgcacctt tttcttcgtg gcctccgaga
     4441 tcttcgagct gtcgcgcaac gcgagcgaaa ctgccgaggg tgacagctgc gcgagccgcg
     4501 caagttgacg aaggttcatg agtgaacagt tgactgttcc ctaccgtcga aggcaaggcg
     4561 aggcacc
//
LOCUS       DASPLT010000041         1182 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_103047, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000041 DASPLT010000000
VERSION     DASPLT010000041.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1182)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1182)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1182
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_103047"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1182)
                     /locus_tag="VEA63_00485"
     CDS             complement(<1..>1182)
                     /locus_tag="VEA63_00485"
                     /inference="COORDINATES: protein motif:HMM:NF021534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2264 domain-containing protein"
                     /protein_id="HYD82482.1"
                     /translation="APTLDPYRHWQSQARALLVPLTKLFQPGRASLAIRGPASANGAN
                     ADRLETLARPLLLAAHYLQSAPEAGSEAFRANVAQWFREGLVAGTTPSSPEAWGPDAN
                     YHQLHVEMGLLCIALQIAPDQLWTPLTRAEQDQIAHWLSSARHSGYVDNNHYFMGIHI
                     LEFLRAHGYGRSGDAAMVDTYFERLEFMRRGNGWFQDGINQAFDYYNAYAFNFYGLWW
                     AHLHGNQNPARAERWREWARSFTRDYEHFFAASGEHAAFGRSITYRFNSIAPFGLSVL
                     ENCTDLPLGRIRRLCTRNLDFFLQQPIYQEQGCLSVGWTDRFDASAELYTGAGSPYWA
                     AKGFSPLLLPPTHAFWQTPEEPLASERGDHAHVIAPAGLVVRSTGDEVEILNAGSQVS
                     HT"
BASE COUNT          225 a          310 c          438 g          209 t
ORIGIN      
        1 tgtgtgcgag acctgagaac cggcgttgag gatttcgact tcgtcgccgg tgctacgcac
       61 cacgagcccg gcaggtgcga tcacgtgggc gtggtcgccg cgttcactgg cgagcggttc
      121 ttcgggcgtc tgccagaacg cgtgcgtcgg tggcaggagg agcggcgaaa aacctttcgc
      181 ggcccaatac ggagaaccgg ctcccgtgta gagctcggcg gaagcatcga agcgatcggt
      241 ccagccgacc gacaggcagc cttgttcctg atagatcggt tgctggagga agaagtcgag
      301 attgcgcgtg cagagccggc gaatgcggcc gagcggaagg tcggtgcagt tttcgagaac
      361 ggagaggcca aagggagcga tgctgttgaa gcgatacgtg atcgagcgac cgaacgcggc
      421 gtgttcgccg ctcgcggcaa aaaagtgttc gtagtcgcgc gtgaaactac gtgcccattc
      481 gcgccagcgt tcggcgcgcg cggggttttg gtttccgtgg aggtgggccc accagaggcc
      541 gtagaaattg aaggcgtaag cgttgtagta atcgaacgcc tggttgatgc cgtcctgaaa
      601 ccagccgttg ccgcgacgca tgaattcgag gcgttcgaaa taggtgtcga ccatcgccgc
      661 atcgccggaa cgcccgtagc cgtgcgcgcg aaggaactcg aggatgtgaa tccccatgaa
      721 gtaatggttg ttgtcgacgt agccggagtg acgcgcggaa ctcagccagt gcgcgatctg
      781 gtcttgctcg gcgcgagtca gcggcgtcca gagttggtcg ggcgcgattt ggagtgcgat
      841 gcacaacaga cccatctcga catggagctg gtggtagttc gcatcagggc cccacgcttc
      901 gggactgctg ggggtggtgc cggcgacgag accttcgcga aaccattgtg cgacgttggc
      961 gcgaaacgcc tccgagccag cttcgggagc cgactgcagg taatgggctg cgaggagcaa
     1021 aggacgggcc aacgtttcga gccggtcggc gttggcgccg ttggcggaag cgggaccgcg
     1081 gatcgcgagc gaggcgcgac cgggttgaaa gagttttgtg agcgggacga ggagcgcgcg
     1141 cgcttgcgat tgccagtggc ggtagggatc gagcgtaggg gc
//
LOCUS       DASPLT010000042         1305 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_164700, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000042 DASPLT010000000
VERSION     DASPLT010000042.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1305)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1305)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1305
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_164700"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..977
                     /locus_tag="VEA63_00490"
     CDS             <1..977
                     /locus_tag="VEA63_00490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007360456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYD82483.1"
                     /translation="AFEEINAAGGVLGKKIELFTEDNQTRPGESATAAKKLVTREKVI
                     ALIGEVASGRALEAAPVAQAAKVPMVAPAATNPKVTEIGDYIFRVCFIDPFQGTVMAK
                     FAHEDLKVKKVAILYSVSNAYSVGLAKYFRETFAGSSGEIVAEANFSEGDKDFRAQLT
                     AVKAAGAEAVFVPSYYTEAALIVRQARDLGIDVPFFGGDGWEAPQLLEIGGAALDGCF
                     YSTHFSPQNQDPAVQEFVSRFKTRWDGQTPDAFAALGYDSAYVLVDAIKRAGTTEGPQ
                     LRDALAATKKFRGVTGETTIDANRDASKPAAILAIKDGKLTFLKTVAP"
     gene            1041..1178
                     /locus_tag="VEA63_00495"
     CDS             1041..1178
                     /locus_tag="VEA63_00495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD82484.1"
                     /translation="MFNFERLEVRQKATVYAGPVYYQALHASGEEISRMLSGRRGSLG
                     G"
BASE COUNT          256 a          430 c          390 g          229 t
ORIGIN      
        1 tggcgttcga ggaaatcaac gcggctggcg gcgtgctcgg gaaaaagatc gagctcttca
       61 ccgaagataa tcaaacgcgc cccggcgagt ccgccacggc cgcgaagaaa ctcgtgacac
      121 gcgaaaaggt gatcgcgctc atcggcgaag tcgcctcggg tcgggcgctc gaagccgccc
      181 ccgtcgcaca agccgccaag gtgccgatgg tcgcacccgc cgcgacgaac ccgaaagtca
      241 ccgaaatcgg cgattacatt ttccgcgtct gctttatcga tccctttcaa ggcaccgtca
      301 tggcgaagtt cgcccacgaa gacttgaagg tgaagaaagt cgcgatcctt tacagcgttt
      361 cgaacgccta cagcgtcggc ctcgcgaagt atttccgcga aaccttcgcg ggcagcagcg
      421 gcgagattgt cgccgaggcg aacttctccg aaggcgacaa ggatttccgt gcgcaactca
      481 ccgccgtgaa agccgccggc gccgaagccg tgttcgtccc gagctactac acggaggcgg
      541 ccctcatcgt ccgccaggcg cgcgatctgg gcatcgacgt cccgttcttc ggcggcgacg
      601 gctgggaggc tccgcaactg ctcgagatcg gcggcgccgc gctcgacggc tgcttttact
      661 ccacgcactt ctcgccgcaa aatcaggacc ccgccgtgca ggaattcgtc tcgcgtttca
      721 aaacgcgctg ggacggccag acgcccgatg ccttcgccgc gctcggttac gattcggctt
      781 acgtgctcgt cgatgccatc aagcgcgctg gcacgacgga aggcccgcag ttgcgtgacg
      841 cgctcgcggc cacgaaaaaa ttccgcggcg tgacgggcga aacgaccatc gatgccaatc
      901 gtgacgcctc gaagcccgcg gcgatcctcg cgatcaagga cggcaagctg acgtttttga
      961 aaacggtggc gccctgaggc gacgcgcggc gcgcggagtt gagagttgag ggttgagagt
     1021 tgagagccaa acctccccgc atgttcaact tcgaacgtct cgaggtgcgg cagaaggcca
     1081 ccgtttacgc cggccccgtt tactaccagg cccttcatgc atcgggagag gaaatctctc
     1141 gcatgctgag cggccggcgc ggctcactcg gcggatgagc cgacctctct cgactctcaa
     1201 ccctcaactc tcaacttcgt ccgagtgacc gaattcctcc agcagctcct caatggcctc
     1261 tcgctcggcg cgatttacgc gctgatcgcg ctcggctata cgatg
//
LOCUS       DASPLT010000043         1526 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_287581, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000043 DASPLT010000000
VERSION     DASPLT010000043.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1526)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1526)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1526
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_287581"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..505
                     /locus_tag="VEA63_00500"
     CDS             <1..505
                     /locus_tag="VEA63_00500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD82485.1"
                     /translation="LAVLVLGNLWLLVAPDLLQAFSGRGYRLEAESGLQAANDERARV
                     VFDEHWYDAEHRSDRYWRWADGDATFAVVNPHGRAVIASVEFYLDGIDQRQATVEFGG
                     RKVWTGMIARKHDARIAFDEIELQPGDNVFRITTDRPAKSVGIEAPRPIGFSLRNLRV
                     RLLRFAP"
     gene            complement(555..>1526)
                     /locus_tag="VEA63_00505"
     CDS             complement(555..>1526)
                     /locus_tag="VEA63_00505"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HYD82486.1"
                     /translation="KNRLGDTTYAARLQEENPYRVNVVHIDPPAVPDIEHHHGRRFLA
                     GKYNVAYWAWELTEFPDSWVPSFAYFDEVWCPSDFTRSAIAMKSPLPVFTMPHAISFE
                     RPVGNHRADFNLPADKFLFLFLYDLNSYSARKNPAAILAAFRASGLAGHGAALVIKVQ
                     NAADNPEDFAALEAAVRDLPGTVLITGTLSRADIYRLEAACDCFVSLHRSEGFGLAIA
                     ESMYLGKPVISTDWSATAEYVTAQTGCPVRYTLATIEKNHGPYVKGQQWAEPDVAHAA
                     EWMRRLFNDRALASALGRAAQHEIETRFSPAAIGARYRQRLQSIAMF"
BASE COUNT          265 a          378 c          592 g          291 t
ORIGIN      
        1 gctggcggtg ttggtgctcg gtaatctttg gctgctcgtg gcgccggatt tattgcaggc
       61 gttttccggg cgcgggtatc gtttggaagc ggagagcgga ttgcaggcgg cgaacgatga
      121 gcgcgcgcgc gtggtgttcg acgagcactg gtatgatgcg gagcaccgga gcgaccgata
      181 ttggcgttgg gcggacggcg acgcgacgtt tgcagtggtg aacccgcacg ggcgagcggt
      241 gatcgcgtcg gtggagtttt atttggatgg catcgatcaa cggcaggcga cggtcgaatt
      301 tggcggccgc aaagtttgga cggggatgat tgcccgaaag cacgatgcgc ggatcgcgtt
      361 cgacgagatt gaactgcagc cgggcgataa tgtgtttcgc attacaaccg atcggccggc
      421 gaagagcgtg gggattgagg cgccgcggcc gatcgggttt tcgctgcgga atctacgggt
      481 gcggctgttg cggtttgcac cgtgaggggc gggcggaatg atgatggtcg ggaatcgctg
      541 caagcagcct aacttcaaaa catcgcgatc gattgcaggc gctggcggta gcgggcgccg
      601 atggcggcgg gagagaaacg ggtttcgatc tcgtgctgcg cggcgcgacc gagggccgac
      661 gcgagggcgc ggtcgttgaa gaggcggcgc atccattcgg cggcgtgcgc gacgtcaggc
      721 tcggcccatt gctggccttt cacgtagggg ccgtgatttt tttcgatcgt cgccagcgtg
      781 tagcggacgg ggcagccggt ttgcgccgtg acgtattcgg cggtcgcgga ccagtcggtc
      841 gatatgaccg gtttgccgag atacatgctc tcagcgatgg cgaggccgaa accttcggag
      901 cggtgaaggg agacgaagca atcgcacgcg gcctcgaggc ggtagatgtc ggcgcgcgag
      961 agcgtgccgg tgatgagcac ggtgcccggc aagtcgcgca cggcggcttc gagcgcggcg
     1021 aagtcctccg ggttgtcggc ggcgttttga actttgatga cgagtgcggc gccgtggccg
     1081 gcgagcccag aggcgcgaaa cgcggcgagg atggcggcgg gattttttcg cgcggagtag
     1141 gagttgaggt cgtagagaaa caggaaaagg aatttgtcgg ccggcagatt gaagtccgcg
     1201 cggtggttgc cgacgggacg ttcgaacgaa atcgcgtgcg gcatggtgaa caccggcagc
     1261 ggggatttca tcgcgatcgc ggagcgcgta aaatcgctcg ggcaccagac ctcgtcgaag
     1321 taggcgaagc tcggcaccca cgagtcggga aactcggtga gctcccaggc ccagtaggcg
     1381 acgttgtatt tgccggcgag gaagcgtctg ccgtgatggt gctcgatgtc agggactgcc
     1441 ggcggatcga tgtgaaccac gttcacacga tacgggtttt cctcctgtag gcgggcggcg
     1501 taggtggtgt cgccgaggcg gttttt
//
LOCUS       DASPLT010000044         1020 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_103120, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000044 DASPLT010000000
VERSION     DASPLT010000044.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1020)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1020)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1020
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_103120"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1020)
                     /locus_tag="VEA63_00510"
     CDS             complement(<1..>1020)
                     /locus_tag="VEA63_00510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009509460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HYD82487.1"
                     /translation="QFDAGETQRCADVTVGDRKATLSLRVPRPKLWSPKSPHLHTVRV
                     ALCDPSAKKETDDARVRFGLRRIEARAGKLWLNGELLQLRGYNRHEWHPNFGPCTPTL
                     QMIADLQLLRDLGCNFIRGSHYPQDQRFLDLCDEFGFLVWEENLGWGQREKTFTSEKF
                     RSDHDTSLRAMVRASYNHPSVIIWGFLNEAGTDAAYVRPAFEQTVATLRALDPTRLIS
                     YASMFAFTDRCFDLVDLIALNLYPGWYGCEGAEAPLELIGPHLQKCIEHIDTAGFSDK
                     PAIVSEIGAEGLYGWHDAHDGFFTEEYQAAYLRRTCQAVLENPRWSGIALWHFSDVRT
                     YGGGWS"
BASE COUNT          219 a          334 c          284 g          183 t
ORIGIN      
        1 gaccagccac cgccgtaggt ccggacgtcg ctgaaatgcc agagcgcgat gccactccat
       61 cgcgggtttt cgagcaccgc ctggcacgtc cggcgcagat acgcagcctg atattcctcg
      121 gtgaaaaagc cgtcatgcgc atcatgccag ccgtagaggc cttcggctcc gatctccgaa
      181 acgatggccg gcttgtcgct aaatcccgcc gtatcgatat gctcgataca tttctgcaga
      241 tgagggccga tcagctcgag cggcgcctcc gcaccttcgc agccatacca acccggataa
      301 agattcagcg cgatcaaatc gacgagatcg aaacaacggt cggtaaacgc aaacatgctc
      361 gcatagctga tcagccgcgt cggatcgagc gcgcgcagcg tcgccaccgt ttgctcgaag
      421 gccggacgca cataagccgc gtccgtccca gcctcgttga gaaaacccca gataatcacc
      481 gagggatgat tgtagctcgc gcgcaccatc gcgcgcaacg aggtgtcgtg atccgagcga
      541 aacttttcac tcgtgaacgt cttctcgcgc tggccccagc cgaggttttc ctcccacacg
      601 agaaacccaa actcgtcgca gagatcgagg aagcgctgat cctgcgggta atgcgaaccg
      661 cggatgaagt tgcaccccag atcgcgcagc aactgaaggt cggcgatcat ttgcagcgtc
      721 ggcgtgcacg ggccaaaatt cggatgccat tcgtgacgat tgtaaccacg cagctgcaac
      781 aactcgccgt tgagccacag cttcccggcg cgcgcttcga tccgacgcag gccaaatcgc
      841 acgcgcgcgt cgtcggtttc ttttttcgcg ctcggatcgc acagcgcgac gcgaaccgta
      901 tgaagatgag gcgacttggg cgaccacagc tttggacgcg gcacccgaag actgagcgtc
      961 gccttgcgat cccccaccgt cacatccgcg catcgttgcg tttccccggc gtcgaactga
//
LOCUS       DASPLT010000045         3874 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_225895, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000045 DASPLT010000000
VERSION     DASPLT010000045.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 3874)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3874)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3874
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_225895"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..449
                     /locus_tag="VEA63_00515"
     CDS             <1..449
                     /locus_tag="VEA63_00515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="topoisomerase C-terminal repeat-containing
                     protein"
                     /protein_id="HYD82488.1"
                     /translation="ELIKGFTSKKGRPFDAFLKREGERISWEFPPRAPKLDKDGKPIV
                     RKAKAPPDLSSAKVIGQSKTFNGEILEAGDSYYVRRPDQDNRIVFTMKRHICAKEIPA
                     GEVQNLLENGRTNLIEGFMSKRMTPFSAYLVLSKNKAKADFEFPPR"
     gene            642..1625
                     /locus_tag="VEA63_00520"
     CDS             642..1625
                     /locus_tag="VEA63_00520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD82489.1"
                     /translation="MRSAKNYVIVFLAGTTLACAVLAWRQYQELISLRAAALNLREAP
                     SWPKRLDTASANSIDGGSAAMAVEAESAEHDGAAIDPSDPATASVHARRERGPRGPNM
                     MDDPEVQKLMAVQRKAGLDGRYAALFRMLNLPPEQLDQLKNLLVDKSTAMADIMAVAR
                     EQGINRRDDPAMFNQLIADAQAEIDANIRTTLGEVGYAQYLDYERTLPQRSVASQLEQ
                     RLSYSSTPLTPDQTAQLVKILANTNPSSSTGTPTTGRNFGGGPLVTDAAINQALGTLA
                     APQIEALRELQREQQLQAELGAAMRQRFQRGQPMPIVPAIPVTPATKTPGG"
     gene            1762..2712
                     /locus_tag="VEA63_00525"
     CDS             1762..2712
                     /locus_tag="VEA63_00525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007359498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HYD82490.1"
                     /translation="MIVTTAQLFKHAYGKYAVGAYNINNAEQAMGLFKGAIQSQAPFI
                     IQISKGARKYTDKKMLEAIIRAAADIFPDAIFAVHLDHGDEETCYDCINSGFYSSVMI
                     DASHDPFEKNVEITKRVVDAAHAKGLSVEAELGMLGGVEEDIKVDEGHAMLTNPAEAE
                     DFVKKTGCDSLACAIGTSHGAFKFKGKQSLHFDVLKQIQTRLPNFPLVMHGSSSVPKD
                     EVDRINKAGGQIKDSMGVDPNEYLPAAKLGVTKVNIDTDGRLVWTRVHREYFRDKPGD
                     FDFRPPGKIFIEEYAKFIASRNQLLGSAGQLADLRKSLGK"
     gene            3070..>3874
                     /locus_tag="VEA63_00530"
     CDS             3070..>3874
                     /locus_tag="VEA63_00530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD82491.1"
                     /translation="MTIGATTYRDVEIRSINARTIILTHAGGMASVRLRDLPPELQQR
                     FNYDPAADVEAKTVNPAPPPRKPAARSNASPNKNLSSAANPVAELLRQFGQPAIVQPR
                     VDLRPKFFELELGVKNQGRRPSCAIFAIVSALEFQSAALTGSAEKFSEEYLTWATWRT
                     VQRIPASSSSADLADTPADEDADLGFTLREVVAALRGYGIPLQSSMPNTFGRSIGAIA
                     DPPAVIVDEARRHQRVFVHQIPGRDNASRINNLVHTLNAGVPVVVGMSWP"
BASE COUNT          762 a         1327 c         1069 g          716 t
ORIGIN      
        1 ccgagttgat caaaggcttc acctcgaaaa aaggccggcc gttcgatgcg tttctcaaac
       61 gcgaaggcga acgcatctcg tgggaatttc cgccgcgcgc accgaagctc gataaagacg
      121 gcaagccgat cgtccgcaaa gccaaggcac cgcccgatct ttcgagtgca aaggtcatcg
      181 gtcagagcaa aacgttcaac ggcgagatcc tcgaggccgg cgattcgtat tacgttcgcc
      241 gccccgatca ggacaaccgg attgtcttca cgatgaagcg ccacatttgc gcgaaagaga
      301 ttcccgccgg cgaagttcag aacctgctcg aaaacggccg cacgaatctc atcgaaggct
      361 tcatgtcgaa gcgcatgacg cccttcagcg cttatctcgt gctctcgaag aacaaggcga
      421 aagccgattt cgaattcccg ccgcggtgag tagccacagc tgcccggccc gtagcgaaac
      481 gcgtgagcgt ttcgttgttt cgtcccgttt cgtttggttc gtttgctcga aaagctcacg
      541 tttttcactg ccaccgacgg cgtgcttggt cgcggtgcgc ctactccgtc gctcctcacg
      601 cttgagtaac ttcgcgagtc ttgcatcgtc cctcctgaag catgcgttct gcaaaaaact
      661 acgtgatcgt ttttctcgcc ggcaccacgt tggcgtgtgc cgtgctggct tggcggcaat
      721 atcaggaatt gatctcgcta cgcgccgcgg cgttgaacct ccgcgaagct ccgagctggc
      781 cgaagcgtct cgataccgcg tcggccaaca gcatcgacgg cggttcggcc gccatggccg
      841 tcgaggctga gagcgccgag cacgacggcg cggcgatcga tccgtccgat ccggccaccg
      901 cctccgttca tgcgcgccgc gaacgcggtc ctcgcgggcc caatatgatg gacgatcccg
      961 aggtgcagaa actaatggcc gtgcaacgca aagcgggtct cgacggccgc tacgccgcgc
     1021 ttttccgcat gctcaatctg ccgcccgagc agctcgacca actcaagaac ctcctcgtcg
     1081 acaagagcac cgcgatggcc gacatcatgg ccgtagcccg cgagcagggt atcaaccgtc
     1141 gcgacgatcc cgcgatgttc aaccagctca tcgcggatgc tcaggcggag atcgacgcca
     1201 acatccgcac gacactcggc gaggttggct acgcgcaata cctcgattac gaacgcaccc
     1261 tgccgcaacg cagcgtcgca tcgcagctcg agcagcgtct cagctacagt tcgacgccgc
     1321 tcacccccga tcagaccgcg caattggtga agattctcgc caacacgaat ccctcttcct
     1381 ccaccggcac gcccaccacc ggccgaaact tcggcggcgg tcccctcgtc accgatgccg
     1441 ccatcaacca ggccctcggc acgctcgcgg cgccgcagat cgaggcgttg cgcgaacttc
     1501 agcgcgaaca gcaattgcaa gccgaactcg gtgccgcgat gcgccagcgc tttcagcgcg
     1561 gacaaccgat gccgattgtg cctgcaatcc cggtcacgcc cgcaacgaag acccccggcg
     1621 gttgagcgcg cgaaccgcgt ttgcttcgcg cggcggccgc gcgtattttg cgcacgccat
     1681 gacacgagat gcgctgagaa ttcacttgtg gctgttctcc gcggctgcct tcgttttcct
     1741 tctagtaaat tccgcacgct tatgatcgtc accaccgcgc agctcttcaa gcacgcctac
     1801 ggcaaatacg ctgtcggcgc ctataacatc aacaacgccg agcaggcgat gggtctcttc
     1861 aagggcgcca tccagtccca ggctccgttc atcatccaga tttcgaaggg tgcgcggaaa
     1921 tacacggaca agaagatgct cgaagcgatc atccgcgcgg cggccgacat tttcccggac
     1981 gccattttcg cggtgcacct cgaccacggt gacgaagaaa cctgctacga ttgcatcaac
     2041 tcgggctttt actcgtccgt gatgatcgat gcctcgcacg atccgttcga gaagaacgtc
     2101 gaaatcacca agcgcgtcgt cgacgccgcg cacgccaagg gtctctccgt cgaagccgag
     2161 ctcggcatgc tcggcggcgt cgaggaagac atcaaggtcg acgagggtca cgcgatgctc
     2221 acgaacccgg ccgaggccga ggacttcgtg aagaagaccg gctgcgattc cctcgcttgc
     2281 gcgatcggca cctcgcacgg tgcgttcaag ttcaagggca agcagtccct tcacttcgac
     2341 gtgttgaagc agatccagac tcgcctgccc aacttcccgc tcgtcatgca cggcagctcc
     2401 tccgtgccga aggacgaagt cgatcgcatc aacaaggccg gcggccagat caaggactcg
     2461 atgggcgtcg accccaacga gtatctcccc gccgccaagc tcggtgtgac gaaggtcaac
     2521 atcgataccg acggccgcct cgtgtggacg cgcgtgcatc gcgagtattt ccgcgacaag
     2581 ccgggcgatt tcgatttccg cccgccgggc aagatcttca tcgaggaata cgcgaagttc
     2641 atcgcctcgc gcaatcagct cctcggcagc gccggtcagc tcgcggatct ccgcaagagc
     2701 ctcggcaagt aagcggccaa ctgctctcat ttcgctttct cggcgggccg atttcggccc
     2761 gccttttttt cgtagcggaa gcaggtcaaa cccgacgtcc cgtcgggttc gatcgaaagc
     2821 gcacacctga accagccgca cgagaccgtc cggctccgcg tccgcagctt cgcacacgtt
     2881 cgccccgact tcgcaaagaa cctgttacaa acaaccctgc acagaaataa tgctgccagc
     2941 caactgcgct cgcgcttacc gtgtggctgt ccgatgcggt ccagcgtgct cctcagtttt
     3001 ctccttctgt tttgcggcgc cctcgccgcg actgagccgg cgtatggtcg tggcacgaaa
     3061 ttcgagagct tgaccatcgg cgccaccacc tatcgcgacg tcgagatccg ctcgatcaac
     3121 gcccgcacga tcatcctcac gcatgccggc ggcatggctt cggttcgctt gcgcgatctc
     3181 ccgcctgagc tgcagcaacg tttcaactac gatcccgccg ccgacgtcga agcgaaaaca
     3241 gttaacccgg cgccgccccc acgaaaaccg gccgcacgct ccaacgcttc gccgaacaag
     3301 aacctctcgt ccgcggccaa tcccgtcgcc gaactcctcc gccagttcgg acaacccgcc
     3361 atcgttcaac ctcgcgttga tttgcgcccg aagttcttcg aactcgagct cggcgtaaaa
     3421 aatcaagggc gtcggcccag ttgcgcgatc ttcgccatcg tgagtgcgct cgagttccaa
     3481 tccgccgcac tcacgggcag cgccgaaaaa ttttccgagg agtatcttac gtgggcaacc
     3541 tggcgcaccg tgcagcgtat tccggcttcg tcgtcttccg ccgacctcgc ggacacaccc
     3601 gccgacgaag acgctgattt gggtttcacg ctgcgcgagg tggtcgcggc gttgcgcggt
     3661 tacggcattc cgctgcaatc ctcaatgccc aacaccttcg gccgctcgat tggtgcgatc
     3721 gccgatccgc ccgccgtgat cgtcgacgag gcgcggcgcc atcaacgggt gttcgttcac
     3781 cagattcccg gccgcgacaa tgccagccgg atcaacaacc tcgtgcacac gctcaacgcc
     3841 ggagtgccgg tcgtcgtcgg catgagctgg ccgc
//
LOCUS       DASPLT010000046         2103 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_164728, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000046 DASPLT010000000
VERSION     DASPLT010000046.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 2103)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2103)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2103
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_164728"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..478
                     /locus_tag="VEA63_00535"
     CDS             <1..478
                     /locus_tag="VEA63_00535"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HYD82492.1"
                     /translation="RPVDSGVSAAENLAGIKVLVVDDDADSLDVVRRILTRRNAEVQT
                     AASVQQALEILAPFGPDVILSDIGMPVHDGYELIRRVRESVSGRTIPAAALTALARSE
                     DRMHALQAGFQTHVAKPVAPAELIAVVRSLAGLRSPPTAPRPAQRERELNNSDPAN"
     gene            complement(640..1146)
                     /locus_tag="VEA63_00540"
     CDS             complement(640..1146)
                     /locus_tag="VEA63_00540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012372865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HYD82493.1"
                     /translation="MAKLTSPRELLVEELKDLYSAENQLLKALPRLAKAATSEELKNA
                     FETHLKQTQGHAERLEKVMKQLDESPKGKTCKAMKGLIEEGKEVMEEDADDAIMDLAL
                     ITAAQKVEHYEIAGYGCARTLAELSGEDKIAAVLQKTLDEEGETDKLLTEIAMGLSFE
                     ATAGVEEE"
     gene            1342..>2103
                     /locus_tag="VEA63_00545"
     CDS             1342..>2103
                     /locus_tag="VEA63_00545"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HYD82494.1"
                     /translation="MANPRASLLLLPIIYLGFISLGLPDGTLGVAWPPVHTELGLPIG
                     LAGTIVIVITLLTGLSGFSSGWVITRWKTGPVVFVSCLLTGSALLAISHAQSLAWLLL
                     AAIPLGLGAGSVDAGLNGYVARHYSGRHMNWLHACWGIGATAGPMVMAHALGTNAGWR
                     GGYFAIAAAQLSLAAIFLATLSLWNRVPERTAAAAAAEHHSGIIPDTPANSRAGWLSA
                     LIFAIYVAIEMTAGVWAGSILVVGRDFSLETAGLCT"
BASE COUNT          306 a          718 c          630 g          449 t
ORIGIN      
        1 ccgccccgtg gacagcggcg tctcggccgc ggaaaatctt gccggcatca aagtgctcgt
       61 cgtcgatgac gatgccgact cgctcgatgt ggttcgccgc attctcactc ggcgtaacgc
      121 cgaggtccaa accgccgcct cggttcagca agcgctcgag atcctcgcgc cctttgggcc
      181 tgacgtgatc ctgagcgata tcggcatgcc tgtgcacgac ggctacgaac ttatccgtcg
      241 cgtgcgcgaa tcggtgagcg ggcgcacgat ccccgccgcc gccttgacgg cgctcgcgcg
      301 atccgaggat cggatgcacg cattacaggc cggttttcaa acgcacgtcg cgaaaccggt
      361 cgcgccggcc gagctgatcg ccgtcgtgcg cagcctcgcc ggtctccgtt cgccgcccac
      421 agcgcctcgg cccgcccagc gagagcggga actgaacaac tccgatcccg cgaactgaaa
      481 gcgggtatgc ctcgcgcgcc gggactaacc cgtatcccac gcagatgcgg ggtgcgcgca
      541 gtctgttgtg gcgaaacgcg tgagcgtctc gtccaggcga gagacgaacg ctcacgcgtt
      601 tcgctacaga tgtgaatttc cgggccgacg aagcgcgact cattcttctt cgacgccagc
      661 ggtggcttcg aagctgagcc ccatcgcgat ttcggtgagc aacttgtccg tctcgccttc
      721 ttcatcgagc gtcttctgca ggactgccgc aatcttgtcc tctccggaca gctcggcgag
      781 cgtgcgcgcg cagccgtagc cggcgatttc gtagtgctcc actttttgcg cggcggtgat
      841 gagcgcgaga tccatgatcg catcgtccgc gtcttcttcc atcacttctt tcccttcttc
      901 gatcagtccc ttcatcgcct tgcaggtttt tcctttgggc gattcgtcga gctgcttcat
      961 gactttctcc agacgctcgg cgtggccctg cgtctgtttg aggtgcgttt cgaacgcgtt
     1021 tttcagctcc tcgctcgtgg ccgctttggc gagacgaggg agcgctttca gcaattgatt
     1081 ttccgcgctg tagagatcct tgagttcttc gacgaggagt tcgcgaggag aagtgagctt
     1141 tgccatgata tttcgtgtag gttggggttg tggttggccg caacggcggc cgggttgacc
     1201 gacaccgacc gaccgatgct cacgcaacca gttccagccg gggaattcgc cccgctgcgt
     1261 cggggattcc accgtagcgc cgcgcgcgcc gaaaacgcgc ttgtcgtttg cccggcccgc
     1321 cgattttcga aacgctgccc cgtggctaac ccacgcgctt cgcttcttct cctgccgatc
     1381 atttatctcg gctttatcag ccttggtctg cctgacggca cgctcggcgt cgcgtggccg
     1441 ccggtgcaca ccgagctcgg tcttccgatc ggcctcgccg gcacgatcgt gatcgtcatc
     1501 acgctgctca ccggcttgtc gggtttctcc agtggctggg tcatcacacg atggaaaact
     1561 ggcccagtcg tgttcgtgag ttgtctgctc acgggcagcg cactgctggc gatctcgcac
     1621 gcgcaaagcc tcgcgtggct cctcctcgcc gcgattccgc tcggcctcgg cgccggctcg
     1681 gtcgacgccg gcctcaacgg ctacgtcgcg cgtcactaca gcgggcgcca catgaactgg
     1741 ctgcacgcgt gctggggtat cggcgcaacc gccggcccga tggtgatggc gcacgcgctc
     1801 ggcaccaacg ccggctggcg cggaggttat tttgccatcg ccgcggcgca gctttccctg
     1861 gcggcgattt ttctcgccac cctctcgctc tggaatcgcg tgcccgagcg caccgccgcc
     1921 gcagccgcgg ccgaacatca ctccggcatc attcccgaca cgccggccaa ctcgcgcgcc
     1981 ggttggttgt ccgcgctcat tttcgcaatc tacgtcgcga tcgaaatgac cgctggcgtc
     2041 tgggcgggtt caatcctcgt cgtcggccgc gatttctcgc tcgaaaccgc cggcctctgc
     2101 acc
//
LOCUS       DASPLT010000047         1102 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_144161, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000047 DASPLT010000000
VERSION     DASPLT010000047.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1102)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1102)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1102
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_144161"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1102)
                     /locus_tag="VEA63_00550"
     CDS             complement(<1..>1102)
                     /locus_tag="VEA63_00550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD82495.1"
                     /translation="NMRRQSTSTRVRGLADASVSRNLFSSVIPFDSYNINRVEINRGA
                     NSVLFGLGSPAGIINYNIVDAMWRNENTVELRVDEYGSFRSVLDINRVLLEDKLALRV
                     IGLNEETSYKQDPAFRDDRRYFAALNYRPFKDTSLSVSFERGWIDSSLPRQDPPRDYF
                     THFFTTGMVSVPNNVDDRDKPANSAYITIDSGTSTLRIFDGPQSNGTGAAFLNSASGA
                     LNPLATNPARRNSFSYTRRAMQNGREFLANVFDDPRGVNAFALGLVDPTVFDFFNNNI
                     DGLASSQYGEIEAFNATLRQQFLNGNAGIEIGYDKQSYDSGYYDALDGIRGNALMLDI
                     SGGELAYATPGDPSSGQAENPNYLRPVVGTRGA"
BASE COUNT          213 a          308 c          360 g          221 t
ORIGIN      
        1 gcgccgcgcg tgccaacgac cgggcggagg taattgggat tctccgcctg gcccgatgag
       61 ggatcgcccg gcgtggcgta ggcgagttcg cccccgctga tatccaacat gagcgcgttg
      121 ccgcgaatgc cgtccagcgc atcgtagtat ccactatcgt aggactgttt gtcgtagccg
      181 atttcgatgc cggcatttcc gttcaggaac tgctgcctca gtgtcgcgtt gaacgcttcg
      241 atttctccat actgggagga cgccaggcca tcgatgttgt tgttgaaaaa gtcgaagacc
      301 gtgggatcga cgaggccgag cgcgaaggca ttcacgccac gaggatcgtc gaaaacgttg
      361 gccagaaact cacggccgtt ctgcatcgcg cgccgcgtgt aggaaaaact gtttcgacgc
      421 gcgggattgg tagcgagagg attgagcgcg ccgctagccg agttgagaaa cgccgcgccg
      481 gtcccgttgg actgaggccc gtcgaagatg cgcaaggtgg atgttccgct gtcgatcgtg
      541 atgtaggcgc tgttcgctgg cttgtcgcgg tcatcgacgt tgttcggcac ggacaccatg
      601 ccggtggtga agaagtgcgt gaagtaatcg cgcggcggat cctggcgagg cagagacgag
      661 tcgatccacc ctctctcgaa gctgaccgag agagacgtgt ccttgaacgg ccggtaattc
      721 aacgcggcga aataccgccg gtcatcccga aaagccgggt cctgcttgta gctcgtttcc
      781 tcgttcaaac cgattacgcg caacgccagc ttgtcctcga gcagcacgcg gttgatatcc
      841 agcacgctgc gaaagctgcc gtattcgtcg acgcggagct cgaccgtgtt ctcgttccgc
      901 cacatcgcat cgacgatgtt gtagttgatg atgcccgccg gcgaaccgag gccgaacagc
      961 acactgttgg cgccgcggtt gatttcgacg cggttgatgt tgtagctgtc gaacgggatg
     1021 accgacgaaa agagattgcg cgaaacggat gcgtcggcga ggccgcggac gcgggtcgaa
     1081 gtcgactgcc ggcgcatgtt gg
//
LOCUS       DASPLT010000048         1384 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_82646, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000048 DASPLT010000000
VERSION     DASPLT010000048.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1384)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1384)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1384
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_82646"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..534
                     /locus_tag="VEA63_00555"
     CDS             <1..534
                     /locus_tag="VEA63_00555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007364114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle suffix domain-containing
                     protein"
                     /protein_id="HYD82496.1"
                     /translation="AHGNYRELRSFQKAEIVYDLTYRFCERFLRKGDRTVDQMVQAAR
                     SGKQNILEGSKASGTSKEMELKLTNVARASLEELLADYEDFLRMRRAPVWEKDAKEAL
                     FVRRLGGRPGVSYEAFREFAETRAGEVVANIARCLIHQANYLLDQQIRRLEQDFLKDG
                     GLRERMTRSRLARRRGE"
     gene            complement(571..>1384)
                     /locus_tag="VEA63_00560"
     CDS             complement(571..>1384)
                     /locus_tag="VEA63_00560"
                     /EC_number="1.1.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009508783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HYD82497.1"
                     /translation="LAVNRARLSNTPAIFWLDEKRAHDAQIIAKVKTYLAQHDTAGLD
                     IKILAPAAACQATLERLVKGQDTISVTGNVLRDYLTDLFPILEVGTSAKMLSIVPLMN
                     GGGLFETGAGGSAPKHVQQFTEENFLRWDSLGEFFALAASFEHLGITQNHAKAKVLAE
                     TLDEANGKFLEHDKSPARKVGAGIDNRGSHFYLALYWAQALAAQSKDAELKKVFTPVA
                     EKLAANEKQIVSELVAVQGKPADIGGYYQPDDKKASAALRPSATLNQILAAL"
BASE COUNT          255 a          350 c          486 g          293 t
ORIGIN      
        1 gcgcacggga attatcggga gctgcggtcg tttcagaagg cggagatcgt ttatgatctg
       61 acttatcgct tttgcgaacg gttcctgcgg aagggcgatc ggacggttga ccaaatggtg
      121 caggctgccc gctccggaaa acaaaacatc ctcgagggga gcaaggcctc gggcacttcg
      181 aaggaaatgg aactcaagct caccaatgtc gcacgcgcga gccttgagga attgctcgcc
      241 gactatgaag atttcttgcg gatgcgaaga gccccggtgt gggagaaaga cgcgaaggag
      301 gcgctgtttg tgcgacggtt gggcgggaga ccgggtgtga gctacgaggc gtttcgcgag
      361 ttcgcggaaa cgagagcggg agaagtcgtg gcgaacatcg cgcggtgcct gatccaccag
      421 gcgaattatc tgttggatca gcagattcgc cggctggaac aggatttttt gaaggacggc
      481 gggctgagag agcggatgac gcggtcgcgg ctcgcgaggc ggagagggga ataggtccta
      541 taggacttat aggacctatg ggacttatgc tcagagtgcg gccaggatct gattgagcgt
      601 cgcgctcggg cgcagcgccg ccgaggcttt tttgtcgtcg ggctggtagt agccgccgat
      661 gtcggcgggc ttgccttgga cggcgacgag ctcgcttacg atttgttttt cgttggcggc
      721 gagtttttcg gccacgggcg tgaagacttt tttcagctcg gcgtctttcg actgcgcggc
      781 gagcgcctgt gcccagtaga gcgcgaggta gaaatgactg ccgcgattgt cgatgccggc
      841 gccgactttg cgcgcgggag acttgtcgtg ctcgaggaac ttgccgttcg cttcgtcgag
      901 ggtctcggcg aggactttgg ccttcgcgtg gttttgcgtg atgccgaggt gctcgaagct
      961 cgcggcgagc gcgaagaatt cgccgaggct gtcccagcgg agaaaattct cttcggtgaa
     1021 ctgctgcacg tgcttcggcg cggagccgcc ggctcccgtt tcgaacaggc cgccaccgtt
     1081 catcagcgga acgatagaga gcatcttcgc actcgtgccg acttcgagga tcgggaaaag
     1141 atcggtgaga tagtcgcgga gaacgttgcc agtgacgctg atcgtgtcct gacctttgac
     1201 gagccgctca agtgtcgcct ggcaggcggc ggcgggagcg aggatcttga tgtcgaggcc
     1261 cgccgtgtcg tgctgcgcca gatacgtttt caccttggcg ataatctgcg cgtcgtgcgc
     1321 gcgtttttcg tccagccaga aaatcgcagg cgtattcgaa agtcgggcac ggttgaccgc
     1381 gagt
//
LOCUS       DASPLT010000049         1014 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_246519, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000049 DASPLT010000000
VERSION     DASPLT010000049.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1014)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1014)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1014
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_246519"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(154..729)
                     /locus_tag="VEA63_00565"
     CDS             complement(154..729)
                     /locus_tag="VEA63_00565"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:TIGR02228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HYD82498.1"
                     /translation="MHSTPAFRLVSTAFGIRIALVVVAAVMVAASGAQAQVAVARPIS
                     GKRETRQTAMLSPSSGLDARAALAAANTIASHIPGARVFTVMATGSMRPTFDEKAFLV
                     VEPTPYENLQIGDVITFRHAKMGAPIVHRIFEKRGDAFWTKGDHNSRPDNEYVTRANY
                     GMRVVAVIYGREDGSRVAGVATQRNATAMLN"
BASE COUNT          187 a          381 c          276 g          170 t
ORIGIN      
        1 aatcgtcgtg agcatcgccg cacgctgagc cactcaccgc gtcgcggcaa ttcccgagtg
       61 aacctacgcc gtcgctggtc acccgctcac aacacaccct cccgcaccct cctcctccct
      121 cgcgtcttta cgaagcaccg cgccgcgctc cgctcaattc agcatcgccg tcgcgttgcg
      181 ctgcgtcgcc acacccgcga cgcggctgcc gtcttcgcgc ccgtagatca ccgccacgac
      241 gcgcatgccg taattcgcac gcgtcacata ttcgttatcc ggccgcgaat tgtgatcgcc
      301 cttggtccag aacgcatcgc cgcgcttttc aaaaatccga tgaacgatcg gcgcgcccat
      361 cttggcgtga cgaaacgtga tcacgtcgcc gatctgaaga ttttcgtagg gcgtcggctc
      421 gacgacgaga aacgctttct cgtcgaacgt cggccgcatg ctgcccgttg ccatcaccgt
      481 gaaaacccgc gctccgggaa tgtgcgaggc aatcgtgttc gcagccgcga gcgccgcgcg
      541 tgcgtcgagt cccgacgacg gcgagagcat cgccgtctgc cgcgtctcgc gcttcccgct
      601 gatcggccgg gcaaccgcca cctgcgcctg cgcgccactc gccgccacca tcaccgccgc
      661 caccaccacg agcgcaatgc gaattccgaa agccgtgctg acgaggcgga aagcgggtgt
      721 ggagtgcatg cccagagcgt agctccgggc gcgaaatcac ggcatccgcc ccgacccgta
      781 gatgggtcta cggaatatcc gcagaccgtc gtaacgcgct cccgctcctc gcctcacccg
      841 tgaccgctcc gcgaaaatct ttagacaatt tggcgccaac ttccggcggt tattccgcac
      901 caaaaattgc gccgcgcgcc agccgcagaa tcggcgatgg cccagcatcc aacgtcacgt
      961 catacacgcg aaatcacggg tgagccgctc ggtgaaccgc cgtcgcgccc aagc
//
LOCUS       DASPLT010000050         1356 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_103177, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000050 DASPLT010000000
VERSION     DASPLT010000050.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1356)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1356)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1356
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_103177"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            400..>1356
                     /locus_tag="VEA63_00570"
     CDS             400..>1356
                     /locus_tag="VEA63_00570"
                     /inference="COORDINATES: protein motif:HMM:NF017197.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF748 domain-containing protein"
                     /protein_id="HYD82499.1"
                     /translation="MLLVMAGLLLAVRFIGSPIATSLANRKLMEMPEVVGRVDDVTLA
                     LWRGAIDVRDLVLHLRGHEDEPPLAHIRKATMRIAPSALFSGKLGASVIVDGAEFHAV
                     KRERIEDPLKAAEEVGDEVQEKKEQAARWQDALRDTFPMELAKLEVKDARVVFLDRTH
                     QPTPELVLERLHVVAHDLQNRPKANDDPLPAKIEITGVTTGNGELRSSIQVNPIAEQP
                     RFTMDFELRGMQLPPLNNFLRAYANADVSRGSFEVFVEVDAEGGAYNGYVKPMFHDLE
                     FRTASDEDKNFAEKLKGKAVAAVASVFKNDEADQVASKAPFAG"
BASE COUNT          263 a          382 c          457 g          254 t
ORIGIN      
        1 ctgcgtcgcg cggaacggcg agctgcgggt tgggtggttt cgagtttttc gatttcatgt
       61 gagaccgtag cgcagatggg cggcgatgtc gtgacatgga tttcccgtgt cagccgtgga
      121 gaagttcgcc gaggctgctg gtgtttgcgg cagcgatgag cagtgcagtt tgctttgtct
      181 gtggcgccga catttgcagg ttgcggcgcg ggagcgaagc ggtggcggaa caggaggctg
      241 agggagcgcg acttcgatga ttggcatccc tcaagcggag acacgcccat gagcctcacg
      301 atgttttccc gcctgcggcg ccgacgcttc gcgtccgctt catccacgca ttcgatctcg
      361 cggagacgaa ctcatccgat cgcgcgaaag ttcgggatcg tgctcctcgt gatggcggga
      421 ctgttgcttg ctgtccggtt catcggctcg ccgatcgcga cgtcgctcgc caaccggaaa
      481 ctcatggaga tgccggaagt ggtgggccgg gttgacgacg tgacgctcgc gctctggcgc
      541 ggcgcgattg acgttcgtga cctcgtgctt cacctccgcg gccacgaaga cgagccgccg
      601 ctcgcgcaca tccgcaaggc cacgatgcgc atcgcgccga gtgcgttgtt tagcggaaaa
      661 ctcggcgcaa gcgtgatcgt cgatggtgcg gagtttcacg cggtgaaacg cgagcggatc
      721 gaggatccac tgaaagctgc cgaagaggtc ggcgatgagg tgcaggaaaa gaaggagcaa
      781 gcggcgcgct ggcaggacgc actgcgcgac acgttcccga tggagctcgc caaattggaa
      841 gtgaaggacg cgcgtgtggt cttccttgac cgcacgcatc agcccacgcc cgagctggtg
      901 ctcgaacgac tccacgtcgt cgcgcacgat ctgcagaatc ggccaaaggc caacgacgac
      961 ccgctcccgg caaaaatcga gatcaccggc gtgacgacgg gcaatgggga gctgcgttca
     1021 tcgatccagg tcaacccgat cgcggaacaa ccgcggttca cgatggattt cgaactgcgg
     1081 gggatgcaat tgccgccgtt gaacaatttc cttcgcgcgt atgcgaatgc cgatgtctcg
     1141 cggggctcct tcgaagtctt tgtcgaggtg gacgcggaag gcggcgctta caacggctac
     1201 gtgaaaccca tgtttcacga tctcgagttc cggacggcct cggacgaaga caaaaatttt
     1261 gccgagaagc tgaaggggaa ggcggtcgcc gcggtcgcat cggttttcaa aaacgacgaa
     1321 gccgatcaag tggcaagcaa ggcgccgttc gccggg
//
LOCUS       DASPLT010000051         1232 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_21691, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000051 DASPLT010000000
VERSION     DASPLT010000051.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1232)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1232)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1232
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_21691"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..451)
                     /locus_tag="VEA63_00575"
     CDS             complement(<1..451)
                     /locus_tag="VEA63_00575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009509688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HYD82500.1"
                     /translation="MLPRTFLTQLTPMRIAEALRRIEAQIWRPLPDACAVTQTESFRE
                     HRTLENLKGADFSPIAEPRFFWGPKYSQRWFRVTLPPASNADKRYLLWQDQAEATAYI
                     DGVPYSGLDLAHRFCPLPDTTTELHIEAVCIRTGIWLDGTAPALDEEG"
     gene            complement(473..682)
                     /locus_tag="VEA63_00580"
     CDS             complement(473..682)
                     /locus_tag="VEA63_00580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD82501.1"
                     /translation="MPWLDWTLVLLSLAAVVAMGAYARRYVKGVAGFMSGDRSAGRYL
                     LTVDHRNLDELPHAAAGSAPSRAFS"
BASE COUNT          226 a          334 c          452 g          220 t
ORIGIN      
        1 tgccttcttc gtcgagagcg ggcgcggtgc catcgagcca aatgccggtg cggatgcaga
       61 cagcttcgat gtgcagctcg gtcgtcgtgt cgggcaacgg acaaaagcgg tgcgcgagat
      121 cgagcccgga gtagggcaca ccgtcgatgt aggcggtggc ctcggcctga tcctgccaga
      181 ggaggtaacg tttatcggcg ttggacgcgg gcggcagggt gacgcgaaac cagcgttgcg
      241 aatatttcgg gccccaaaag aaacgcggct cggcgatcgg cgagaaatcg gcgcctttca
      301 gattctccaa cgtgcggtgc tcgcggaaac tctccgtctg cgtgacggcg caagcgtcgg
      361 gcaaaggacg ccagatttgc gcttcgatgc gacgcagcgc ttcggcgatg cgcatcggag
      421 tcaattgggt gagaaaagtt ctgggcagca tcttgaaaga ttaaggcgcg cgttaggaga
      481 acgcgcgcga tggcgcggag ccggctgcgg cgtgcggcaa ctcgtcgagg ttgcggtggt
      541 cgacggtgag aagataacga ccggcggagc ggtcgccgga catgaagccg gcaacgcctt
      601 tgacgtagcg ccgcgcgtag gcgcccatcg cgacgacggc ggcgagggag aggagaacga
      661 gcgtccagtc gagccacggc atgacgagtt atcgctgagg gcggcgcgag gaggctgtgc
      721 cgagtagggc ggacttcagt ctgccatttt gacgtgtgga tgaaggcggg ctgaagcccg
      781 ccctacgggg gtgcgttcgt tgcgcacggg cgcttctttg ggcgcgacgc gcggccgagg
      841 cgacggccgc gatcgcgagc agaacgacag gggcctgccg atggcgaagc gaaaacatgc
      901 gaaggtggcg gtgcgtagtt ctagtttgaa gaggcggcgt catcgtagcc cggaacccgc
      961 tgcgtaatct tgttcgattt tgggcagttg ttgtgttttt ttctcatgca tcgatgaagg
     1021 aaactctgcg aaccccacgc gccccgtcgc cgattcgcgc gccccggctt cagctcacgg
     1081 tcaagcggcg cgcagaatcg ttcgtgtgcc ggcgttgcgt ggaccgcggc tatcacgcgc
     1141 cggaggaagc gttcgacgcg ccgtatcatt ttcatcccga agtcgagttg ctgctgatgg
     1201 agtcgagccg ggggacgcgg tatgtcggcg ac
//
LOCUS       DASPLT010000052         2303 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_41792, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000052 DASPLT010000000
VERSION     DASPLT010000052.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 2303)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2303)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2303
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_41792"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..972
                     /gene="hemG"
                     /locus_tag="VEA63_00585"
     CDS             <1..972
                     /gene="hemG"
                     /locus_tag="VEA63_00585"
                     /EC_number="1.3.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004729 - oxygen-dependent
                     protoporphyrinogen oxidase activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protoporphyrinogen oxidase"
                     /protein_id="HYD82502.1"
                     /translation="ARTSDLNLADFVRSHFGSSFVNYALNPFVTGVYAGNPQKLSARH
                     AFPKLWELEQTHGSLLRAQVALAKTRRARGASAPSIFSFRDGLQTLTDTLAARLPAGA
                     VTLNTTLDAIIPGEKWSVVWHDATATHTQAFDAIVVALPAHALAKLRIGALGERPLAA
                     LDAMEHPPVSSLFLGFRREQVAHPLDGFGALVPAVEKRSLLGALFSSTLFPQRAPDRH
                     VALTVLIGGTRQPDLASLPLDRLLATVQPDLAALLGVSGDPVFAHHTFWPRAIPQYNL
                     GHEQFLAAMASAEHQHPRLFIGGQARDGISLPACIAAGEKLAVRAAA"
     gene            1103..>2303
                     /locus_tag="VEA63_00590"
     CDS             1103..>2303
                     /locus_tag="VEA63_00590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007362683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HYD82503.1"
                     /translation="MAHDLKDTLQLPKTDFPMRAGLAQREPGRVAHWEKIGLYAAIQE
                     KSRATNKTFVLHDGPPFTNGDVHIGTALNKTLKDLILRYKSMRGFRTPYVPGWDCHGL
                     PIEQKVSREIRDQKLTVTTAELRARCDAFAETWIAKQTAQFKRLGVLADWKNEYKTKA
                     PAYEADILCVFAAFVEKGLIYRSKKPVYWSIPFETALAEAEIEYKDHVSPSIWVKFAV
                     PAAEAAKFNLPSDKPLFVVIWTTTPWTIPANLAIALHPEVEYVVADLGAERIIVAQAL
                     LAQVAAAAKLPADPTIIQTVPGKSLEKLAARHPFIDRASPIVLADYVTTESGTGCVHT
                     APGHGSEDYLTGLKYGLEIYCPVGDDGKYLDDGKIPADLVGLTTLESVEDLAAKKTSP
                     ANIAVLKK"
BASE COUNT          418 a          895 c          584 g          406 t
ORIGIN      
        1 gcccgcacga gcgacctcaa tctcgccgat ttcgtccgtt cgcacttcgg ctcaagcttc
       61 gtcaattacg cgctcaaccc tttcgtcacg ggcgtctacg ccggcaatcc gcaaaaactt
      121 tccgcccgcc acgcgttccc caaactttgg gaactcgagc agacgcacgg ttcgctgctc
      181 cgcgcccaag tcgcgctcgc gaaaacccgc cgcgctcgcg gtgcgtccgc gccgtccatt
      241 ttttcgtttc gcgacggtct ccaaacgctc accgacacac ttgccgcccg tcttcctgct
      301 ggcgccgtca cgcttaacac caccctcgac gcgatcatcc ccggcgaaaa atggagcgtc
      361 gtctggcacg acgccaccgc cacgcacacg caagccttcg acgcgatcgt cgtcgcgctg
      421 cccgcgcacg cgctcgccaa actacgcatc ggcgcgctcg gcgaacgccc gctcgccgcg
      481 ctcgacgcga tggagcaccc gcccgtcagc tcgctctttc tcggcttccg ccgcgaacag
      541 gttgcacacc cgctcgacgg cttcggcgca ctcgtgcccg ccgtcgaaaa gcgttcgctt
      601 ctcggcgccc tgttttcgtc cacgcttttt ccgcaacgcg cacccgatcg acacgttgcg
      661 ctcaccgttc tgatcggcgg cacgcgccag cccgatctcg catcgctgcc gctcgatcga
      721 ctgctcgcca ccgtccagcc tgacctcgcc gcccttctcg gcgtgtccgg cgatccggtt
      781 ttcgcacacc acacgttttg gccgcgcgcg atcccgcaat acaatctcgg ccacgagcag
      841 ttcctcgccg cgatggcgtc ggcggagcac caacacccgc gccttttcat cggcggccag
      901 gctcgcgacg gcatctcgct ccccgcttgc atcgccgccg gcgaaaagct cgccgtccgc
      961 gccgccgcct aagctgtggg aggggcttta cgccccgact cccgctccgt ccgcagtcca
     1021 cccgccgcgg tcatcaacct ccccggccaa ctgcccttga ctcacctccc gcccggccta
     1081 gcctcgcgca cttttctttg gcatggccca cgacttgaaa gacacgctgc agcttccaaa
     1141 aaccgatttc ccgatgcgcg ccggtctcgc gcaacgcgaa cccggccgcg ttgcccactg
     1201 ggaaaagatc ggcctctacg ccgccattca ggagaaaagc cgagccacca ataaaacctt
     1261 cgtcctccac gacggcccgc ccttcaccaa cggcgacgtc catatcggca ccgcgctcaa
     1321 caagacactg aaggatctca tcctgcgcta caaatcgatg cgcggattcc gcacgcctta
     1381 cgtccccggc tgggactgtc atggcctgcc gatcgagcaa aaagtttccc gcgagatccg
     1441 cgaccagaag ctcaccgtca ccaccgccga actccgcgct cgttgcgatg cgtttgccga
     1501 aacctggatc gcgaagcaga ccgcgcagtt caaacgcctc ggcgtcctcg cggattggaa
     1561 aaatgaatac aagacgaagg cgcccgctta cgaggccgac atcctgtgcg tcttcgccgc
     1621 cttcgtcgaa aaaggcctga tctaccgcag caagaaaccc gtttactggt cgatcccctt
     1681 cgagaccgcg ctcgccgaag ccgagatcga atacaaggac cacgtcagcc cctcgatctg
     1741 ggtgaagttc gccgtcccgg ccgccgaggc cgcgaaattc aatctccctt ccgacaaacc
     1801 gctcttcgtg gtcatctgga caacgacgcc ttggaccatt cccgcgaatc tcgcgatcgc
     1861 gctgcatccg gaggtcgaat acgtcgtcgc cgatctcggc gccgagcgca tcatcgtcgc
     1921 gcaagcgctt ctcgcgcagg tcgccgctgc cgccaagctt cccgctgatc cgacgatcat
     1981 ccaaaccgtc cccggcaaat ccctcgagaa actcgccgcc cgccaccctt tcatcgaccg
     2041 tgcgtcgccc atcgtgctcg ccgactacgt cacgaccgag agcggcaccg gctgcgtgca
     2101 tacggcgccg ggccacggct ccgaggatta tctcaccggt cttaaatacg gcctcgaaat
     2161 ttactgtccg gtcggcgacg acggcaaata cctcgacgac ggtaaaatcc ccgccgacct
     2221 cgtcggtttg accacgctcg aatccgtcga ggatctcgcc gcgaagaaaa cttcgcccgc
     2281 caacatcgcc gttctcaaga aac
//
LOCUS       DASPLT010000053         1475 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_1040, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000053 DASPLT010000000
VERSION     DASPLT010000053.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1475)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1475)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1475
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1040"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..936)
                     /locus_tag="VEA63_00595"
     CDS             complement(<1..936)
                     /locus_tag="VEA63_00595"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HYD82504.1"
                     /translation="MATPNISGTLRRSLLIKVISKPAPSREDVSSVIELTSSKIVEAL
                     QAQSMTFYLVEGNDIVFKNVYYSPTLWAQEPALEKNLQETATKLLALKLPLGTGVVGK
                     VIASGEPIFFRNTETQAPMMVSLAQTTGFDVRSMLTVPLKTNISIGAIQVLNKELSAG
                     TGGEFTDKDLALLQEVAEYSATLLHRMLDPKFQLSADDTAKFVAKLTDLPLVTKADEL
                     EIDEMLVQSIGDAIIRREGVFPHKRLSPSSISALMTNPLDYGKREAFSQATEMSIEDV
                     SVVSATLFESLLKKYFKDSSAATGEKIDIGSVAEEI"
     gene            1020..1451
                     /locus_tag="VEA63_00600"
     CDS             1020..1451
                     /locus_tag="VEA63_00600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HYD82505.1"
                     /translation="MLRWLQQFFRLGGADDAGARGERLAAEWLHRERGFAVVARNWRD
                     PRDRRREIDLVCRDGEVLVFVEVKARAAGALVPGYFAVNAGKKKVMRQAIDAYLKRLA
                     KPPRTFRFDVVEVAHDTNGSGGAPEVLHFENIPLFPKQYRG"
BASE COUNT          247 a          403 c          527 g          298 t
ORIGIN      
        1 gatttcctcg gcgacggaac cgatgtcgat tttttcgccg gtggcggcgg agctgtcctt
       61 gaaatatttt ttgagcagcg attcgaagag cgtggctgaa acgacgctga catcctcgat
      121 gctcatctcg gtcgcctgcg aaaacgcctc gcgttttccg tagtcgagcg ggttggtcat
      181 caaggccgag atcgagctcg gcgagagacg cttgtgcggg aacacacctt cgcggcggat
      241 gatcgcgtcg ccaatgcttt ggacgagcat ctcgtcgatc tcgagttcgt cggccttcgt
      301 gacgagcggg aggtcggtga gcttcgcgac aaacttcgcg gtgtcgtcgg cgctgagctg
      361 gaacttcggg tccagcatgc ggtgcaacag ggtcgcggag tattcggcga cttcctggag
      421 cagcgcgagg tccttgtccg tgaactcgcc gccggtgccg gcggagagct ccttgttcaa
      481 cacctggatc gcgccgatcg agatgttggt cttcagcggc accgtgagca tcgagcgcac
      541 atcgaagccg gtcgtctgcg cgagcgacac catcatgggc gcctgcgtct cggtgttgcg
      601 gaagaaaatc ggttcgccgc tggcgatcac tttgccgacg acgccggtgc cgagcgggag
      661 tttgagcgcg aggagtttgg tcgcggtctc ctggagattc ttctcgagcg cgggttcctg
      721 cgcccagagc gtcggcgagt agtagacgtt tttgaaaacg atgtcgtttc cctcgaccag
      781 gtagaacgtc atcgactgtg cctgcagcgc ctcgacgatt ttcgacgacg tcagctcgat
      841 cacggagctc acgtcttcgc ggctcggcgc gggtttcgag atgaccttga tcagcagcga
      901 gcggcgcagg gtgccggaga tatttggggt ggccatgttg cgctggacga ggttctggga
      961 ttgtcaccgg cgcgaaccgc tcgcaatgag aaagagccgg cgcgagccgc gcgacgtcca
     1021 tgctgcgatg gttgcaacaa tttttcaggc tcggcggcgc tgacgatgcg ggcgcgcgcg
     1081 gtgaacgtct cgcggccgaa tggttgcacc gcgagcgagg cttcgcggtc gtggcgcgca
     1141 actggcgcga tccgcgcgac cgccggcggg agatcgacct cgtgtgccgc gacggcgagg
     1201 tgctggtgtt cgtggaggtg aaagcgcgcg cggccggcgc gctcgtgccc ggctattttg
     1261 cggtgaacgc gggaaagaag aaggtgatgc ggcaggcgat cgatgcgtat ttgaagcggc
     1321 tggcaaagcc accgcggacg tttcgtttcg atgtggtgga ggtggcgcac gacacgaacg
     1381 ggagcggagg tgcgccggag gtgttgcact tcgagaacat cccgctgttt ccgaaacagt
     1441 atcgcggata ggtcgccggc tcgggcgtgg taggg
//
LOCUS       DASPLT010000054         1693 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_206085, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000054 DASPLT010000000
VERSION     DASPLT010000054.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1693)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1693)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1693
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_206085"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1663)
                     /locus_tag="VEA63_00605"
     CDS             complement(<1..1663)
                     /locus_tag="VEA63_00605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HYD82506.1"
                     /translation="MHKATQLTLEMPATSRLRLFNHGRFAKLIRPYSGGLYGIDMYGA
                     GDRQAYETNYIFNSIRPESRASGFGAMLAETNNHGGPGHQFASTFWRLLGNGAKGMMY
                     FTPGFVGAPKGSDWDKFSMIDRATGRPKDRWFYAARWAAAVHRSERFWSEAVPASDAP
                     RVAMLMPRRDILLSERSERNPGEGRYAYARNHRWMVYRWLREQGYWVDVLPYEKLTDG
                     YLVDYSALLLVGAEHLTSTECETVARYVAAGGILVADTMPGYFDQHHRVLNAIASPAG
                     LVVHRHADLRPVAFDFGGRTVEGVTAYTVNAETARVLARNADGEPLALLQAFREGRIL
                     SLPFELGSLVLKGKDTAVVSLQSEQEPTAGAEQYAAYVDEFLIGEWLAGLLAQAGLRP
                     AYAAIYPSASDARIVRVEQPYVDAAGNVAVVIGNRAQLHPQEILPAGAVELPLPGGTW
                     THAFWAAAETDDLVQIEVTSLGEGRHRVELPSIASAGIVYLFNDHTPLLGIVSRSKDT
                     EFAASETARVKPGVAFALPVQLVNPNDDPLPAGSLTVQVPAGWSVT"
BASE COUNT          324 a          599 c          495 g          275 t
ORIGIN      
        1 gcgtgaccga ccagccggcc ggcacctgga ccgtcaacga gcccgcgggg agcggatcgt
       61 cgttgggatt caccagttgc accggcaacg cgaacgccac gccaggcttc acccgcgctg
      121 tctcactcgc cgcgaactct gtgtctttcg agcgcgaaac gatgcccagc agaggcgtgt
      181 ggtcgttgaa caaatacacg atgccagcag acgcgatcga cggcagctcg acgcgatgcc
      241 gtccttcgcc gagcgacgtc acttcgatct gcacgaggtc atccgtttcc gccgccgccc
      301 aaaacgcgtg cgtccaagtt ccgccgggca acggcagctc caccgcgccc gccggaagaa
      361 tctcctgcgg atgcaactgc gcgcggttgc cgatcaccac cgccacgttg cccgcggcat
      421 ccacataagg ttgctccacc cgcacgatcc gcgcgtcgga cgccgacgga taaatcgccg
      481 catacgccgg tcgcaaccca gcctgcgcca gcaaacccgc cagccactca ccgatgagaa
      541 actcgtctac gtaagccgca tactgctcgg cgccggcggt cggctcttgc tcggattgca
      601 atgaaaccac agccgtgtcc ttgcctttaa gaaccagcga cccgagttcg aacggcagcg
      661 acaggatgcg tccttcgcgg aacgcttgca gcagcgcgag cggctcgccg tccgcgttgc
      721 gcgccagcac acgggccgtc tctgcgttga ccgtgtaagc cgtgacgccc tcgaccgtgc
      781 gcccgccaaa gtcgaacgcc acggggcgca ggtcagcgtg gcgatgaacc acgaggcccg
      841 ccggcgacgc gatcgcgttg agcacccgat gatgctggtc gaaatagccg ggcatcgtat
      901 ccgccaccaa aataccaccc gccgccacat aacgcgccac ggtctcgcac tccgtcgaag
      961 tcagatgctc ggccccgacg aggagcagcg cggaatagtc cacgagatag ccgtccgtca
     1021 gcttttcgta gggcagcacg tcaacccaat agccttgctc acgcagccag cgatagacca
     1081 tccagcgatg attgcgcgcg taggcatagc gcccctctcc cggatttcgc tcggagcgct
     1141 ccgacagcag gatgtcacgg cgcggcatca gcatcgcgac gcgcggcgca tccgacgccg
     1201 gcacggcctc cgaccagaag cgttcgctgc gatgcaccgc cgcggcccag cgcgcggcat
     1261 aaaaccagcg atccttcggg cgtccggtcg cgcgatcgat catcgaaaac ttgtcccagt
     1321 cgctgccttt cggtgctccc acaaaacccg gcgtaaaata catcatgcct ttcgcgccgt
     1381 tgcccagtaa tcgccagaag gtgctggcga actggtgtcc cggcccgccg tggttgttgg
     1441 tctcggccag catcgcaccg aagcccgacg cgcggctctc cggacgaatg ctgttgaaaa
     1501 tatagttggt ctcgtaggcc tgacgatctc ccgcgccata catgtcgatc ccatacaagc
     1561 cgccactgta aggccggatc agctttgcaa agcgtccgtg attgaacagc cgcagccggc
     1621 tcgtcgcggg catttcgagc gtgagctggg tggccttgtg cacgacgcca cgcttctgcg
     1681 gatccgcgtc atg
//
LOCUS       DASPLT010000055         2333 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_246606, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000055 DASPLT010000000
VERSION     DASPLT010000055.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 2333)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2333)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2333
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_246606"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1183)
                     /gene="ccoO"
                     /locus_tag="VEA63_00610"
     CDS             complement(<1..1183)
                     /gene="ccoO"
                     /locus_tag="VEA63_00610"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF011055.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit II"
                     /protein_id="HYD82507.1"
                     /translation="MATFEGPLLSIKSVNALGHYTDWIIGHVHAGALGWNGFMAAGMA
                     YWLLPRLWNKPLHSVSLANLHFWLGTVGILLYVAAMWTSGITQGLMLNATTDNGTVLA
                     YPNFVDTLNAIRPMMLMRVVGGGLYLTGFLLMGYNVWKTVRGAQPVNGTVEVFEEDPV
                     STPAPGRVGVFGTFINPPVLFTVLAFGFACAWMFGNDFVSIAGFVGMLLCVIIAYSHF
                     ESRGKAWASWYDRLLVNTAPFTILTFIAVAAGGLIQIVPTVMAHKAANLEDRRQVPYT
                     PLELAGRDIYVREGCYNCHSQMIRTLVPDVLRYGEYSRLGESIYDHPYQWGSKRTGPD
                     LARVGGKYPNVWHLRHMEDPRAISAGSNMPAYPWLLANDTDIGALPSKLSVQRTLGIP
                     YP"
     gene            complement(1263..2135)
                     /locus_tag="VEA63_00615"
                     /pseudo
     CDS             complement(1263..2135)
                     /locus_tag="VEA63_00615"
                     /inference="COORDINATES: protein motif:HMM:NF012343.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
BASE COUNT          493 a          701 c          770 g          369 t
ORIGIN      
        1 tcggatacgg aatgccgagc gtgcgttgaa ccgacagctt cgacggcagc gcgccgatgt
       61 cggtgtcgtt cgccagcagc cacggataag cgggcatgtt ggaaccggcg gagatcgcgc
      121 gcggatcctc catgtggcgg agatgccaga cgttcggata cttgccaccg acgcgcgcga
      181 ggtcgggacc cgtgcgcttc gagccccatt gataagggtg atcgtaaatc gattcgccga
      241 ggcgcgagta ttcgccgtag cggagaacat ccggcaccag cgtgcggatc atctgcgagt
      301 gacagttgta gcagccttcg cgcacgtaga tgtcccgacc ggcgagctcg agcggcgtgt
      361 aaggcacctg ccggcggtcc tccagattcg ccgccttatg ggccatcacc gtcggcacga
      421 tttgaatgag accgcccgcg gcgacggcga tgaaggtgag gatcgtgaac ggcgccgtgt
      481 tgacgagcag ccggtcatac caactcgccc aagctttgcc gcgcgactcg aaatgcgaat
      541 acgcgatgat cacgcacagc agcatgccga cgaaaccggc aatgctgacg aagtcgttgc
      601 cgaacatcca cgcgcaggcg aaaccgaacg cgagcacggt gaagagcacc ggcgggttga
      661 tgaaggtgcc gaagacaccg acgcgaccgg gagccggcgt agacaccgga tcctcttcga
      721 aaacctccac cgtgccgttg accggctgcg caccgcggac ggtcttccag acattgtagc
      781 ccatcagcag gaagccagtc agatagagcc cgccgccgac cacgcgcatg agcatcatcg
      841 gacggatggc gttgagcgtg tccacaaagt ttggatacgc gaggaccgtg ccattatccg
      901 tcgtggcgtt gagcatcagg ccctgcgtga tgccgctcgt ccacatcgcg gcgacgtaga
      961 gcaggatgcc aacggtcccg agccagaagt gaaggttcgc gagcgaaacg gagtgcagcg
     1021 gcttgttcca caaccgcggc agcagccagt aggccatgcc ggcggccatg aagccgttcc
     1081 agccgagcgc gccggcgtga acgtggccga tgatccagtc cgtgtaatgg ccgagcgcgt
     1141 tgaccgactt gatcgaaagc agcggccctt cgaacgtcgc catgccgtag aacgtcaccg
     1201 ccgcggaact tgagcacggg atcggtgcgc agcttgtgcc acgcgccgcg caacgtgagc
     1261 aggccgttca gcatgccacc ccacgacggc gcccagagca tcagtgagaa cgtcatgccg
     1321 agcgtctgca gccattcggg cagcgcggtg ttgagcagat ggtgcggacc ggcccagata
     1381 tagacgaaga ccagcgacca gaaatggatc accgaaagcc ggtaggagta aaccggccgc
     1441 tccgccgcct tcgggatgaa ataatacatg atgccgagga tcggcgtcgt gaggaagaac
     1501 gccacggcgt tgtgcccata ccaccattgc acgaggccgt cctgcacgcc tccaaaaatt
     1561 ggatacgaat gcgtgaggct cgtcggcagc gacaggtgat tgacgatgta gagcatcgcc
     1621 acggtgatga tcgtcgcgat gtagaaccag atcgcgacgt agaggctgcg ctcgcggcgc
     1681 ttagcgagcg tccagaagaa gttcaccgcg aacacgaccc agatgaacgc gaccgcgatg
     1741 ttgagcggcc agatcagttc cgcgtattcc ttgccgcgcg tgaggccgag cgtgagcgtg
     1801 atcgccgcgg cgacgatgat cgcctgccag ccccagaaat gcagcgccga gaggaagtcg
     1861 ctcgcgagcc gcgccttcac cagccgctgc gtcgaatagt agatgccggc gaacatcatg
     1921 ttgccgacga acgcgaagat cacggcgttc gtgtgcaacg gacgcagccg gccgaagctc
     1981 agccaggagg tcttgaagtt gacctcccag aaattgagct gcgtcgcgat cagcacgccg
     2041 acgagcatgc ccacgatccc ccacacgagg gtcgcgagca gaaactggcg gacaactttg
     2101 tcgttgaatt ccagggtgat tttacggtcg gacatcgatc gagggagcgt tgggacgcgc
     2161 ggctgtgtgc tgcgtgctgc gttaaggtcg gctcactccg cgcgcggcgg atgtgagtct
     2221 gtagcggtga cgcggatacg agcgaggcgc gggctctcct cggccagagg cagcagcgag
     2281 tcgcgctcgg cgctgctgag gcttccgcgc gcgtgttcgc gcaggaagaa gat
//
LOCUS       DASPLT010000056         1315 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_41879, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000056 DASPLT010000000
VERSION     DASPLT010000056.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1315)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1315)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1315
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_41879"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1065)
                     /gene="nusA"
                     /locus_tag="VEA63_00620"
     CDS             complement(<1..1065)
                     /gene="nusA"
                     /locus_tag="VEA63_00620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HYD82508.1"
                     /translation="MSSEILSVLEYMEKEKGIPRADMIATIANALKTAAQKGVNSGQE
                     LKIEINPKNGQLHAWSLLKVVDSVSNPRTEVHVERAQALKPGVLIGETIEKEIDPSTL
                     GRIAAQTARQAVMQRLRQFEKDRIFDDFKDQVGNIVTGTVRRRERNDLYIDLGKAEAV
                     MPGKEQVPGEEYQPGERIRCLLLAIESTPRGPEIILTRGSPKFVRRLFELEVTEIADG
                     TVKIEAFAREPGYRTKIAVTSADPKVDPVGACVGARGARVKTIVRELGGEKIDIIKYF
                     SDPREMIIEALKPAVPREIVLDEKTHRILLKVATDDLAVAIGRKGQNARLTSRLIGWR
                     LDIEEFKAVGADPEGDAKRKL"
BASE COUNT          236 a          436 c          376 g          267 t
ORIGIN      
        1 gagcttgcgc ttcgcgtcgc cttccgggtc ggcgcccacg gccttgaatt cttcgatgtc
       61 gagccgccag ccaatgagcc gcgacgtgag ccgggcgttc tggcccttgc gaccgatcgc
      121 gaccgcgagg tcgtccgtgg ccactttcaa aagaatgcgg tgcgtctttt catcgagtac
      181 gatctcgcgc ggcaccgccg gcttgagcgc ctcgatgatc atttcgcgcg gatcggagaa
      241 atacttgatg atgtcgattt tctcgccgcc gagttcgcgc acgatcgtct tcacgcgcgc
      301 accgcgggcg ccgacgcacg cgccgaccgg atcgactttc gggtccgcgc tcgtcaccgc
      361 gatcttcgtc cggtagcccg gctcgcgcgc aaacgcctcg atcttcaccg tgccgtcggc
      421 gatttcggtg acctcaagct cgaacaaacg gcgcacgaac ttcgggctgc cgcgagtgag
      481 gatgatttcc ggaccgcgcg gcgtgctctc gatcgcgagc aacaaacagc ggatgcgctc
      541 gcccggctga tattcttcgc ccggcacctg ttccttgccc ggcatcaccg cctcggcttt
      601 gccgagatcg atgtagaggt cattgcgttc gcgacggcga accgtgcccg tgacgatgtt
      661 gccgacctgg tctttgaaat cgtcgaagat gcgatccttc tcaaactgac gcagccgctg
      721 catcaccgcc tgacgcgcgg tttgcgcggc aatgcggccg agcgtcgacg gatcgatttc
      781 cttctcgatc gtctcaccga tgagcacgcc cggcttcaac gcctgcgccc gctccacgtg
      841 cacttccgtg cgcggattgc taaccgaatc gaccaccttg agcagcgacc acgcgtgcaa
      901 ctggccgttc ttcggattga tctcgatctt aagctcctgg cccgaattca cccccttctg
      961 cgccgccgtc ttcagagcat tcgcgatcgt cgcgatcatg tcggcgcgag ggataccctt
     1021 ctccttttcc atgtattcga ggactgaaag tatttcgctg ctcataggac ttaagattga
     1081 gggaagaaga tgagaaaaaa aaagcgggcc gcgtggccca cttccttgca aagtgagtgg
     1141 tgtagagccg aaaaacgtat tgaccggcgg gaaaccttgt caagccccgc gccctgcggg
     1201 aatcccttgc gagcctcgcg acacctcgcg cgcaatcatc cagccgagca gaccttgcgc
     1261 agcgccatga cacgccggct gcgcacgtcc ggaaaacact ccgagttcgc gcaac
//
LOCUS       DASPLT010000057         1318 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_267049, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000057 DASPLT010000000
VERSION     DASPLT010000057.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1318)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1318)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1318
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_267049"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(379..534)
                     /locus_tag="VEA63_00625"
     CDS             complement(379..534)
                     /locus_tag="VEA63_00625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009636 - response to toxic substance
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="entericidin A/B family lipoprotein"
                     /protein_id="HYD82509.1"
                     /translation="MNIFNKTHLRRFSVLALLASVLGFAASGCRTMDGAGQDIERAGE
                     KIQENAR"
     gene            734..>1318
                     /locus_tag="VEA63_00630"
     CDS             734..>1318
                     /locus_tag="VEA63_00630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HYD82510.1"
                     /translation="MPTAQFPAENADGDFQRQEDAFRDWISADGSTAYPPTPGRYHLY
                     VSLACPWATRVAIVRRLLGLEPVIGLTVVDPVRDERGWAFRDGPGFSRDPVNGFAFLA
                     EAYRATAPNFNGRVTVPVLWDTVTHRIVNNSEDDICRMLHDAFRAYHPADIDLFPDDL
                     RRQQEELSTFIYERVNNGVYRAGFATRQAAYERAC"
BASE COUNT          253 a          503 c          321 g          241 t
ORIGIN      
        1 cgatacgccg cgctactcga tcacctaccg cagccttcgc tgacgccgcg cgcctgcgag
       61 tggagtgtcc tcgaaatcaa atcgcacgga cgcgccgcta gagatcgccg gtagggatgc
      121 ctctccgagg catccgttca gtggcgagaa atactgaatg actacaacac ctgtaggaca
      181 ccctgctagc cgtgctgccg cgccgctcac gcacctcctt ccgaagcccg aacacaacgc
      241 aacgaaggaa cgaagaagcg ttccgcccgc caccgtcgct tcgttccctt cgttaccttc
      301 tgttcaaatc aagcgaccga actcgaactc ccgctcgagt tcggtcacgt cacggcgtct
      361 agccgctccc gctcccgctc aacgcgcgtt ctcctgaatc ttctcgcccg cgcgttcgat
      421 atcctgaccc gccccgtcca tcgtgcgaca accactcgcc gcaaagccga gcaccgaagc
      481 gagcaaagcc agaacggaga accgacgaag atgcgttttg ttgaaaatat tcatagcgcc
      541 gcagtctact ctccacgcaa cgctttcgcc atcggagcgg cgcccgttcg cacccttcgc
      601 tttttcacgg ttcaacaccc gaaaacggaa cagcccgccc cgtcgtcgcg acacccgtcc
      661 cttcacttca tttgaaaaat cgcggcgtct cctcaactca tgtgacgcag ccatcaccgc
      721 atttccgtcc accgtgccca ccgctcaatt ccccgccgaa aacgccgacg gcgatttcca
      781 acgccaggaa gacgccttcc gcgattggat ctccgccgac ggctcgaccg cgtatccgcc
      841 gacccccggc cgctatcact tgtatgtctc gctcgcctgc ccgtgggcaa cgcgcgtcgc
      901 catcgtgcgc cgcctgctgg gactcgaacc agtcatcggt ctcaccgtag tcgatccagt
      961 gcgcgacgaa cgtggctggg cgtttcgcga cggccccggc ttcagccgtg atcccgtcaa
     1021 cggcttcgcc ttcctcgccg aagcctaccg cgcaaccgcg cccaacttta acggccgcgt
     1081 caccgtgccc gttctctggg acaccgtcac tcaccggatc gtgaacaact ccgaagacga
     1141 catctgtcgg atgctgcacg acgcttttcg cgcctatcat cccgccgaca tcgacctctt
     1201 tcccgacgac ctccgccgcc agcaagaaga gctctccacg ttcatctacg agcgcgtcaa
     1261 taacggcgtc taccgcgccg gtttcgcgac ccgacaggcc gcctacgaac gcgcgtgc
//
LOCUS       DASPLT010000058         1661 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_21734, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000058 DASPLT010000000
VERSION     DASPLT010000058.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1661)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1661)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1661
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_21734"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1579)
                     /locus_tag="VEA63_00635"
     CDS             complement(<1..1579)
                     /locus_tag="VEA63_00635"
                     /inference="COORDINATES: protein motif:HMM:NF012356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HYD82511.1"
                     /translation="MALTTASRIPVATYRLQMRGEFPFAAAAEQVAYASSLGISDFYC
                     SPIFLSTPGSTHGYDVNDYRRVNPELGGRSELERLHARLKEQGMGLLLDFVPNHMGIN
                     GPGLLNRWWRDVLENGEHSAHARFFDIDWNAGATEGASQVLVPILEDHYGRVLEAGKL
                     SIIFEEGTLSVAYGDMRFPLRPSSYRAVWADVPGAGELRAVIDAIGNLPHPPAREESS
                     AASTRAALFADLKGRLRASVERDSGLHAKLDARLRALNGAAGQRESFDELDRIVQQQH
                     YRLAFWKAGVHETNYRRFFAIDTLIGLRMENAEVFEECHTLLAELVRGGIVTGVRIDH
                     VDGLRDPKQYLERLQGLAGGGPRDVTKPLYVLVEKILEDDEQLPNDWATHGTTGYEFI
                     AQLAGLLVDARSERRFTNTYAAFCGETAAFDDVVYEKKRLVLEEMFANAVTRLAHDLA
                     DLLRIDRRWRDVTRNELTVAVREIMAAHGVYRTYRRGAEPMSERDRRVVEQACAVALS
                     RNRRLAAAPFELVRDVLS"
BASE COUNT          322 a          620 c          435 g          284 t
ORIGIN      
        1 cggacaacac gtcgcgcacc agttcgaacg gcgccgcggc caaccgccgg ttgcgcgaca
       61 acgccactgc gcacgcctgc tccacgacgc gccgatcgcg ctcgctcatc ggctccgcgc
      121 cgcgtcgata cgtccgataa acgccatgcg ccgccatgat ctcgcgcacc gccaccgtga
      181 gctcgttgcg cgttacgtcg cgccaacgac gatcgatccg caagaggtca gcgagatcgt
      241 gcgcgagacg cgtcaccgca ttcgcaaaca tctcctcgag cacgagccgt ttcttctcgt
      301 aaacgacatc gtcgaacgca gccgtttcac cgcagaacgc cgcatatgtg ttcgtgaaac
      361 gccgctccga gcgcgcgtcc accaacaacc ccgccagctg cgcgatgaat tcgtagcccg
      421 tcgttccgtg cgtcgcccag tcattcggca actgctcgtc gtcttcgaga atcttttcca
      481 ccagcacata cagcggcttc gtcacatctc gtgggccgcc accggcgaga ccctgcaagc
      541 gttcgagata ttgcttcgga tcacgcagcc cgtcgacgtg atcgatgcgc acacccgtca
      601 caattccacc gcgcaccaac tccgcgagca gcgtgtgaca ttcctcgaac acctccgcgt
      661 tctccattcg caatccgatc agcgtgtcga tcgcgaagaa ccgccgataa ttcgtctcgt
      721 gcacgcccgc tttccaaaag gccagccggt aatgctgctg ttgcacaatg cggtcgagct
      781 cgtcgaagct ttcacgctgc cccgcggcgc cgttcagcgc acgcaaccgc gcatcgagtt
      841 tcgcgtgcaa tccgctgtcc cgttcgaccg acgcgcgcag ccttcccttc aaatccgcga
      901 acagcgccgc gcgcgtgctc gccgcactac tctcttcgcg cgccggcgga tgcggcaggt
      961 ttccgatcgc atcgatcacc gcgcgcagtt ctccggcacc ggggacgtcc gcccacaccg
     1021 cgcgatagct cgacggccgg agcggaaaac gcatgtcgcc ataagccacg ctcagcgtgc
     1081 cctcctcgaa tatgatcgaa agttttcccg cttcgagcac gcgcccgtag tgatcttcca
     1141 aaattggcac gagcacctgt gacgccccct cggtcgcacc ggcattccag tcgatgtcga
     1201 aaaaccgcgc gtgcgccgag tgctcgccat tttccagcac gtcgcgccac caccggttca
     1261 acaggcccgg accgttgatc cccatgtgat ttggcacgaa gtccagcagc agtcccatgc
     1321 cctgctcctt caatcgcgcg tgtaaccgct ccaactcgga ccgcccgccc agctcgggat
     1381 tcacgcgccg gtaatcgttc acgtcgtagc catgcgtgct gcccggcgtc gaaagaaaaa
     1441 tcggcgaaca ataaaaatcg gaaatcccca gcgacgacgc ataagccacc tgctccgccg
     1501 ccgccgcgaa cggaaactcc ccacgcatct gcaagcgata cgtcgcgacc ggaatacgac
     1561 tggccgtggt aagcgccatc aggatacgcg gccgccgccc tcctctgcga ccagcaacac
     1621 cgtcgccggc gcgtcgaaga ccagcgcatc gaccttcggc c
//
LOCUS       DASPLT010000059         1570 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_41895, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000059 DASPLT010000000
VERSION     DASPLT010000059.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1570)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1570)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1570
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_41895"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..407
                     /locus_tag="VEA63_00640"
     CDS             <1..407
                     /locus_tag="VEA63_00640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD82512.1"
                     /translation="YQIVAGQAPNRVYRVAAGVAVTTGVVLLWINGAVGLIGSENNPA
                     NLMYGGVLAIGLIGAAIARLKPLGMARALIVTAVAQFLVPVVGLIVWRPDFSPGVVQV
                     FGLNFAFVLMFAGSALLFRQAAAISSERTRHG"
     gene            complement(554..1165)
                     /locus_tag="VEA63_00645"
     CDS             complement(554..1165)
                     /locus_tag="VEA63_00645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD82513.1"
                     /translation="MNFAEIENTWRSPHNRINPAELEKNKMQLIAELRRRRRATIGLL
                     GVTFLPLAFVTGRLVLHLLHAEAGRDPVDLATEWGVVPFFLLPWIGWLSMVALYRRHR
                     ARHLNYDQSIRASVQALLDENRTERTHCKVIGGLLIASVAVLPIVIQQLRAVGKAGDE
                     ILVPAYVIYPGYVVLMVGWTIWHYRRKLLPRERELEELLNVYR"
     gene            complement(1152..>1570)
                     /locus_tag="VEA63_00650"
     CDS             complement(1152..>1570)
                     /locus_tag="VEA63_00650"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HYD82514.1"
                     /translation="TLLLAMWRAVPAFRGGAQASTFVYRVAHNAALTWRRSQKNYAAR
                     LERFAPLASAELSDAAGAGANDTTREREALEHVYAAIRELPLLDRSLILLHLDGVSYA
                     EIAEIHGLTESNVGARLTRLKQKLTTALEDIAHELR"
BASE COUNT          286 a          479 c          499 g          306 t
ORIGIN      
        1 cgtatcagat cgtggcgggc caggcgccga atcgcgttta ccgtgtcgct gcgggcgtcg
       61 cggtgacgac tggcgtcgtt ctcttgtgga tcaatggagc ggttgggctc atcgggagcg
      121 aaaataatcc cgcgaacctg atgtatggcg gcgtgctggc gatcgggctg atcggcgccg
      181 cgatcgcgcg attgaaaccg ttgggaatgg cgcgggcatt aatcgtgaca gcggtggcgc
      241 agttcctggt gccggtcgtg ggactgattg tttggcgtcc cgatttcagt ccgggtgtcg
      301 tgcaggtgtt cggactgaat ttcgcgttcg tcctgatgtt cgccggatcg gcgcttctgt
      361 tccggcaagc agcggcgatc tcaagcgaac gaactcgcca cggctgagca gaggcacgtc
      421 gcatctgcgg ccaaaaattg gggcatggtt tttttgaacc gctgcagtgt cgcgtcccgc
      481 aagtaacttc agtaattcag cattcttctc cgctgaacgg acgcctcgga gaggcatccc
      541 gactggcgcc acgctaacga tacacgttca acaattcctc caactcgcgt tcgcgcggca
      601 gcagtttccg ccggtagtgc cagatggtcc aacccaccat cagaaccacg tagccgggat
      661 aaatcacata cgccggcacc aggatctcgt cgccggcttt accgactgcc cgcagttgct
      721 ggatcacgat tggcaacacc gcgacactcg cgatgagtaa gccgccgatg accttacaat
      781 gcgtgcgctc ggtgcggttc tcgtcgagca atgcctgcac gctcgcgcga atcgactgat
      841 cgtagttcaa gtgccgcgcg cggtggcgcc gataaagggc caccatcgaa agccagccga
      901 tccacggcag cagaaaaaat ggcacgacgc cccactcggt cgccaggtcg acagggtcgc
      961 ggcctgcctc cgcgtgcagg aggtgcaaca cgagcctgcc ggtaacgaag gcgagcggaa
     1021 ggaaagtgac gcccagcagc ccaatggtcg cgcggcggcg ccggcgcagt tcagcgatca
     1081 gttgcatttt gtttttctcc agctccgccg gattgatgcg gttgtgtggc gagcgccacg
     1141 tgttttcgat ttcagcgaag ttcatgagca atgtcctcca gtgccgtggt gagtttctgt
     1201 ttcaagcggg tcaaccgcgc gccgacgttt gactcggtca ggccgtgaat ctccgcgatc
     1261 tccgcatagc tcacgccgtc caggtgcagc aaaatcagtg agcgatccaa caacgggagt
     1321 tcgcggatcg cggcgtaaac atgctcgagt gcctcgcgtt cgcgggtggt gtcgttcgcg
     1381 ccggcgcccg ccgcgtccga gagttcggcc gaagcgagcg gggcgaagcg ctccagccgc
     1441 gccgcgtaat ttttctggct ccgccgccag gtgagcgccg cgttgtgcgc gacgcgatac
     1501 acgaacgtcg acgcctgtgc gccgcctcga aacgccggca ccgcccgcca catcgcgagc
     1561 agcagcgtct
//
LOCUS       DASPLT010000060         2280 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_62334, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000060 DASPLT010000000
VERSION     DASPLT010000060.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 2280)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2280)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2280
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_62334"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1580
                     /locus_tag="VEA63_00655"
     CDS             <1..1580
                     /locus_tag="VEA63_00655"
                     /inference="COORDINATES: protein motif:HMM:NF025295.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="immunoglobulin domain-containing protein"
                     /protein_id="HYD82515.1"
                     /translation="SSTSDGGVTWSSHTAPGIETYAPYYSVAAGGGRFVLVATGTSTK
                     VWTSPDAATWTEILAGQQTSGKLAYGNGIFVLTGTNYKTSTDGINFTTHPTPTGFTLA
                     ATNPLRFTGGRFLYQQFGLTPSGGFGGMVMASTDGLNWSYLAGTGSGNVFDFVEGNGR
                     IVLVGWSSGGTILAPTRTAVISYLNAPPIEAGPQSQSLALGGSATLSTTATGTLQWQR
                     NGSNLAAASSSSLALSNVQPADAGIYTVNATNGSTVTAQHAIVGLTTSSKVIGTGNEV
                     EANIVHPNGNIFDQVLLTGPAEAITADHAQNQITRTSYIDLDGDIVQVEFSGPGTLSL
                     VLDNPTDPAPPANYNQTVNYMKGHAGIVITGATEQTNVSVFSVGRATAVNQALFKNNV
                     TYDGIADIAFIAISSSNGKFGGVRTANANYFAAKGLTGLYAPGVAFQGPVYIGDIIAS
                     ATATPVLIIGSGSDTRITGGNLDQDNDAAVEVDGLTQLKFTAGGDSHGNTLLAQTNAG
                     VLKDNGVDVTTQIVVNP"
     gene            1785..>2280
                     /locus_tag="VEA63_00660"
     CDS             1785..>2280
                     /locus_tag="VEA63_00660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD82516.1"
                     /translation="MHALPRLLLLFLLSTALLSRAQTPTWTNLDHQLGANFTELNSVA
                     FGNGLFVAIKSPLTGGTLHYATSTDGTTWTARSVATSITSSGSAQRVRFLDGRFLIMP
                     IGLAGGAGVALSLTSTDGISWTTATVGTTTTSPDEMDFGNGRVLAGGSNNYFVGSTNF
                     STWTA"
BASE COUNT          431 a          894 c          568 g          387 t
ORIGIN      
        1 tgagctcgac cagcgatggc ggtgtcacct ggtcttcgca caccgcgccc ggcattgaga
       61 cttacgcgcc ctactattcc gtcgcagccg gcggcggtcg cttcgtgctg gtcgccacgg
      121 gaacgtcgac gaaagtctgg accagccccg acgccgcgac ctggaccgaa atcctcgccg
      181 gccagcaaac ctccggaaaa ctcgcctacg gcaacggcat attcgtcctc accggcacga
      241 actacaaaac cagcaccgac ggaattaact ttaccacgca tcccacgcct accggcttca
      301 cgctcgctgc gaccaacccg ctccgtttca ccggcggccg tttcctttat caacagttcg
      361 gcctgacgcc ctcaggcggt ttcggcggaa tggtgatggc gagcaccgac ggactgaatt
      421 ggtcctacct cgctggcacg ggatccggca acgtgttcga cttcgtggaa ggcaacggtc
      481 gcatcgtcct cgtcggttgg tcgagcggag gcaccatcct ggcgccgacg cgcactgcgg
      541 taatttccta ccttaacgcc ccgcccatcg aagctggccc ccagtcgcaa tccctcgcgc
      601 tcggcggcag cgccacgctg agcacgaccg ccaccggcac gctccaatgg cagcgcaacg
      661 gctccaacct cgccgccgcc tcgagttcgt cgctcgcgct cagtaacgtc cagcccgccg
      721 acgccggcat ctacacggtc aacgccacca acggttccac cgtcaccgct caacacgcga
      781 tcgtcggcct caccacctcc agcaaagtca tcggcaccgg caacgaagtg gaagccaaca
      841 tcgttcatcc caatggcaac atcttcgacc aagtcctcct caccggcccc gccgaagcca
      901 tcaccgccga tcacgcgcag aaccaaatca cccgcacgtc ctacatcgat ctcgatggcg
      961 acatcgttca agtcgagttc tccggccccg gcacgctctc gctcgtgctc gataacccca
     1021 ccgatccggc gccgcccgcc aactacaacc aaaccgtcaa ctacatgaag ggccacgccg
     1081 gcatcgtcat caccggcgcc accgagcaga ccaacgtctc cgtcttcagc gtcggccgcg
     1141 ccaccgccgt taaccaagcg ctcttcaaaa acaacgtcac ctacgacggc atcgccgaca
     1201 tcgcgttcat cgcgatctcc tcttcgaacg gaaaattcgg cggcgtgcgc accgccaacg
     1261 ccaactactt cgccgcgaaa ggtctcaccg gcctctacgc tcccggcgtc gctttccaag
     1321 gccccgtcta catcggcgac atcatcgcct ccgcgaccgc cacgcccgtg ctgatcatcg
     1381 gctccggctc cgacacacgc atcaccggcg gcaatctcga tcaggacaac gacgccgctg
     1441 tcgaagtcga cggcctcacg cagctgaagt ttaccgccgg cggcgactcc cacggcaaca
     1501 cgctgctcgc ccaaaccaac gccggcgtcc tcaaggacaa cggcgtcgac gtcacgactc
     1561 agatcgtcgt caacccctaa catcaccgcg atgtgcgagg ggcttcacgc cccgactcgg
     1621 cgtccgccct ccgtcaccag catccggata cgttccagcc cgcccgcgca acctgggttg
     1681 cgcgggcgtt ttcgtttacc atctcgcgtt tcacaccagc ggctccgctc ctacgcaccc
     1741 atgcgtatgg gccgatttcc tctcgtgcgc tattctcgcg aggtatgcac gcccttcctc
     1801 gactactcct tctcttcctt ctttccacgg ctctcctctc gcgcgcgcaa acgccgacgt
     1861 ggaccaatct cgaccatcaa ctcggcgcaa atttcaccga gctcaacagc gttgctttcg
     1921 gcaacggcct gttcgtcgcg atcaaatcgc cgctgaccgg cggcacgctc cactacgcca
     1981 cgagcaccga cggcacgacg tggaccgccc gcagcgtggc cacgagcatc accagctccg
     2041 gcagcgcgca gcgcgtccgc ttcctcgacg gacgtttcct tatcatgccc atcggtcttg
     2101 ccggcggcgc tggcgtcgca ctctccctca ccagcaccga cggcatttcg tggacgaccg
     2161 cgaccgtcgg cacaaccacg acctcgcccg atgaaatgga tttcggcaac ggccgcgttc
     2221 tcgccggcgg ctccaacaac tatttcgtgg gctccaccaa cttcagcacc tggaccgcgc
//
LOCUS       DASPLT010000061         1065 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_123704, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000061 DASPLT010000000
VERSION     DASPLT010000061.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1065)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1065)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1065
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_123704"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1048)
                     /locus_tag="VEA63_00665"
     CDS             complement(<1..1048)
                     /locus_tag="VEA63_00665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HYD82517.1"
                     /translation="MSSYKYVNFLWDDAKASSLDPVGRLVYRSNILGSDQRITNTGGG
                     NTSSKLIEKDPLTGQPVEVLWVKGSGGDLRTSTRENFSSLYQQKLIDLQKLYAARTDK
                     GLKSPAEDDMVGMYTHATFNLNPRASSIDTPLHSFLPGKHVDHMHPNAIIAIAASKHC
                     EKLTQEIFGGEMAYVPWMRPGFELGLAMQEIARKNPKLRAIMMGQHGFISWADDDKQC
                     YTLTLDLIEKAAAYIESKYAAKGGDATAFGGQKYQTLDEAKRHATLAAILPWLRGQVS
                     QQKRFIGTLQDDAKILRFVNSKDAPRLAELGTSCPDHFLRTKIKPLYVDWNPQAEDVA
                     ALKAKLAAGLDQYRK"
BASE COUNT          171 a          270 c          380 g          244 t
ORIGIN      
        1 ccttgcggta ttgatcgagg ccggcggcga gtttcgcttt gagcgcagcg acgtcctcgg
       61 cttgcggatt ccaatcgacg tagagcggct tgatcttcgt gcgcaggaag tggtcggggc
      121 acgatgtgcc aagctcggcg aggcggggtg cgtctttaga gttaacgaag cgcaggatct
      181 tcgcgtcgtc ctgcagggtg ccgatgaaac gcttttgttg cgaaacttgt ccgcgcagcc
      241 acggcaggat cgcggcgagc gtcgcgtgac gtttcgcttc gtcgagcgtc tggtatttct
      301 gtccaccgaa ggccgtggcg tcgccgccct tcgcggcgta tttggattcg atatacgcgg
      361 cggctttttc gatgagatcg agcgtgagcg tgtagcactg tttatcatcg tcggcccagg
      421 agatgaagcc gtgctggccc atcatgatcg cgcggagctt cggatttttt ctggcgattt
      481 cctgcatcgc gaggccgagc tcgaagccgg ggcgcatcca cggcacgtag gccatctcgc
      541 cgccgaagat ttcctgcgtg agtttctcgc agtgcttcga ggcggcaatc gcgatgatcg
      601 cgttggggtg catgtgatcg acgtgcttgc ccggcaggaa ggagtgcagc ggggtgtcga
      661 tcgaggatgc gcgtgggttc aggttgaagg tcgcatgcgt atacatgccg accatgtcgt
      721 cctcggcggg agatttcagg cctttgtcgg tgcgcgcggc gtagagtttt tgcaggtcga
      781 tcagcttctg ttggtagagc gacgagaagt tttcgcgcgt gctcgtgcga agatcgccgc
      841 cggagccttt gacccagagg acctcaaccg gctggccggt gagcgggtcc ttctcgatga
      901 gtttcgacga cgtgttgccg ccgccggtgt tggtgatgcg ctggtcgctg ccgaggatat
      961 tggagcggta gacgaggcgc ccgacgggat cgagcgaaga ggccttggcg tcgtcccaga
     1021 ggaagttgac gtatttgtag gaggacatgg tgagagaggt gatgg
//
LOCUS       DASPLT010000062         1069 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_185259, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000062 DASPLT010000000
VERSION     DASPLT010000062.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1069)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1069)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1069
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_185259"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1069)
                     /locus_tag="VEA63_00670"
     CDS             complement(<1..>1069)
                     /locus_tag="VEA63_00670"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HYD82518.1"
                     /translation="TFDDGFANTGSCTSSITFIDGEAGILRYRGYPIEQLAEKSSFVE
                     TAYLVMYGELPTKEQRQAFSSRLTTHARLREGMLRFIQNFPRDSHPMAVLSATFNALG
                     AYYPHLASNNHQRDIEHFDEAAAIAISKVRTIAAHTYRVNNGLPFNDPRPDLGYCENF
                     LHLMFSEPYTPYAATPEVASALNLFLLLHADHEQNCSTSTVRMVASAGANLFASISAG
                     VNALWGPLHGGANMAVLEMLQSIHDEGDDGTKFIAAAKAGKGERLMGFGHRVYKNYDP
                     RAKIIKASFYKALGSLGIKDDPLLNIAMKLEEVALNDDYFISRKLYPNVDFYSGLIMR
                     ALGIPLNMFTVMFAIGRMPGW"
BASE COUNT          222 a          336 c          308 g          203 t
ORIGIN      
        1 atccagcccg gcatgcggcc gatggcgaac atcaccgtga acatgttcaa cggaatcccc
       61 agcgcgcgca tgatcagacc agagtagaaa tccacgttcg gatacagctt ccgcgaaatg
      121 aagtaatcgt cgttcaacgc cacctcctcg agcttcatcg cgatgttgag cagcggatcg
      181 tccttgatgc cgagcgagcc gagcgccttg tagaagctcg ccttgatgat cttcgcgcgc
      241 ggatcgtagt tcttgtagac ccggtgaccg aagcccatca gccgctcgcc ctttccggct
      301 ttcgccgccg cgatgaactt cgtgccgtcg tcaccctcgt cgtgaatgct ctgcagcatc
      361 tcgagcaccg ccatgttcgc gccgccgtgc agcggacccc aaagcgcatt cacaccggcg
      421 gagatcgaag cgaaaagatt cgcgcccgcc gacgccacca tgcgcaccgt cgacgtcgaa
      481 cagttctgct cgtgatcagc atggagcaga agaaatagat ttagcgccga tgcgacctcg
      541 ggcgtcgccg catagggcgt gtaaggctcc gaaaacatca ggtgcaggaa attctcgcag
      601 tagccgagat ccgggcgcgg gtcgttgaac ggcaggccgt tgttcacccg atacgtgtgt
      661 gcggcgatcg tgcgcacctt cgaaatcgcg atcgcggccg cctcgtcgaa gtgctcgatg
      721 tcgcgttggt gattattgct cgcgaggtgc ggataatacg cgccgagcgc gttgaacgtg
      781 gcggacagca ccgccatcgg atgcgaatca cgcgggaagt tctggatgaa acgcagcatg
      841 ccttcgcgca aacgcgcatg cgtcgtaagc cggctcgaaa acgcctgccg ctgctccttc
      901 gtcggcagct cgccatacat cacgagatac gccgtttcca cgaaactcga cttctccgcg
      961 agttgctcga tcgggtagcc ccgataacgc aaaatgccgg cctcgccgtc gatgaacgtg
     1021 atcgaactcg tgcacgaacc cgtgttcgca aatccgtcgt cgaacgtga
//
LOCUS       DASPLT010000063         1849 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_42001, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000063 DASPLT010000000
VERSION     DASPLT010000063.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1849)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1849)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1849
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_42001"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..369)
                     /locus_tag="VEA63_00675"
     CDS             complement(<1..369)
                     /locus_tag="VEA63_00675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD82519.1"
                     /translation="MNPKTWIFIRPALAGVFVYLSTTALAQSVDAPPASETSANSADV
                     VVLSPFQVTTEKDRGYRATNSISGTRLDTAIKELPMPIEVITEEFLRDTGSTDLRQSL
                     RYSSGILLQTQNDYGAAAGSF"
     gene            542..>1849
                     /locus_tag="VEA63_00680"
     CDS             542..>1849
                     /locus_tag="VEA63_00680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD82520.1"
                     /translation="MAPARRTLLALALFGLTPPLAFAATAPASTLSTAAAVLSLTAQQ
                     AGTQLPVTVTGVVTAAEPDWQGQFFVQDETGGVFVENFSSPTPQPGDVVTVAGVSHPG
                     AFAPIISKPHWTRISQATLPNPKIVAIEDLESGVEDGQRVEIAGIVRTARPSSGRLDL
                     ELAVGGYRLQIRAPALPDRTPDSLVGAHVRVRGTAATHYNAALRHLTAVAVYVPQPED
                     LTVLESEAVNPFNQPALPLNTVAQYRRGSGSGQRVHVRGTVTLQRLGADVFLQDETGG
                     LRIETAQLESFAPGDQVDAAGFLEYEDYLPLLRDATLRRSGTASDGVRARVVPLAEIM
                     NGFHHARAITLRGKILDRSTRPVSRHVGDFSGVVTTWLIQADDLGFTVEREDRQENAS
                     LSAIPTGAIVEVDGVCFPEIDEAGKLKSLKLLLASPDGLRILER"
BASE COUNT          321 a          674 c          525 g          329 t
ORIGIN      
        1 aaaacttccc gccgcggcgc cgtagtcgtt ctgcgtctgc agcaaaatac ccgagctgta
       61 acgcaaactc tgccgcaaat ccgtcgagcc cgtgtcacgc aaaaattcct ccgtgatcac
      121 ctcgatcggc atcggcagtt ccttgatcgc cgtatcgagc cgcgtgccgg aaatcgagtt
      181 cgtcgcgcga tagccgcgat ccttctccgt cgtcacttga aacggactga gcacaaccac
      241 gtccgcgctg ttcgccgatg tctcggaagc cggcggtgcg tcgaccgatt gcgccaacgc
      301 agtcgtgctc aggtaaacga aaacgcccgc gagtgcgggc cggatgaaaa tccacgtctt
      361 ggggttcata gttggaaagc agccgccggg cgaacttctc cgcaagtggg gtgtgtcagt
      421 tgttcgccgc aggcagcttc gcgcagagta acaaaacgcg gccgaccgcc gcatccctct
      481 ttcgggggga ggagaatgcg tcgacagcgc gtgcacgttt tctaaactcg ctgcattccc
      541 catggctcct gcccgacgca cgttgctcgc gctcgctttg ttcggactga ccccgccgct
      601 cgcgttcgcc gccactgcgc ccgcgtcgac tttgtccacc gccgccgccg tgctctcgct
      661 caccgcgcag caagccggca cgcaactgcc cgtcaccgtc accggtgtcg tcaccgccgc
      721 cgagccggac tggcagggtc aatttttcgt gcaggatgag accggcggcg tgttcgtcga
      781 aaatttctcc tctccgacgc cgcaacccgg cgacgtcgtc accgtcgccg gcgtcagcca
      841 tcccggcgcg ttcgcaccga tcatcagcaa accgcattgg acgcgcatca gccaggcgac
      901 gcttcccaat ccaaagattg tcgcgatcga agatctcgaa tccggcgtcg aggatggtca
      961 acgggtcgag atcgccggca tcgttcgcac cgcgcgcccc agctccggcc ggctcgatct
     1021 cgagctcgcc gtcggcggtt atcgcctgca aatccgcgcg cccgccttgc ccgaccgcac
     1081 gcccgactcg ctcgtcggcg cgcacgttcg cgtgcgaggc accgccgcca cacactacaa
     1141 cgccgcgctt cgccacctca ccgccgtcgc cgtttacgtg ccgcagccgg aagatctcac
     1201 cgtcctcgaa tccgaagcgg tcaacccctt caatcagccc gcgcttccgc tcaacaccgt
     1261 cgcgcaatat cgccgcggca gcggctcggg tcagcgcgtg cacgtccgcg gcaccgtcac
     1321 cctccaacgt cttggcgccg acgttttcct gcaggacgaa accggcggac tgcgcatcga
     1381 aaccgcccaa ctcgaatcct tcgcgcccgg cgaccaggtc gacgccgccg gcttcctcga
     1441 atacgaggac tatcttccgc tgttgcgcga tgcgacgctc cgccgctccg gcacggcgag
     1501 cgacggcgtg cgcgcgcgcg tcgttccgct cgccgaaatc atgaacgggt ttcatcacgc
     1561 gcgcgcgatc acgctgcgcg gaaagatcct cgatcgctcc acgcggccgg tttcgcgcca
     1621 cgtcggagat ttctccggcg ttgtcaccac ttggctgata caggccgacg accttggttt
     1681 caccgtcgaa cgcgaagacc gccaggaaaa cgcctcgctc tccgcgatcc ccaccggtgc
     1741 catcgtcgaa gtggacggcg tttgtttccc ggagatcgac gaagccggaa aactgaaaag
     1801 cctgaaactg ctcctcgcct cgcccgacgg tctgcgaatt ctggagcgt
//
LOCUS       DASPLT010000064         1121 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_82868, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000064 DASPLT010000000
VERSION     DASPLT010000064.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1121)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1121)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1121
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_82868"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..323
                     /locus_tag="VEA63_00685"
     CDS             <1..323
                     /locus_tag="VEA63_00685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD82521.1"
                     /translation="TLDCAATFRQSPGLPVVRILKIVLSILVAIAAVIGGLFTVAVVV
                     VASLAVLLTRRFLARTRPRVSTPSAPAQVRRTPARAPDAIEITATEVPNEENTASREK
                     TPAP"
     gene            complement(409..1014)
                     /locus_tag="VEA63_00690"
     CDS             complement(409..1014)
                     /locus_tag="VEA63_00690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD82522.1"
                     /translation="MTNKSTGCYRARVRLRGGERNVLKAGMLAAGCLLALATVVGCAS
                     GQYTYVTTIGTGQRLEFPLEKGKPANAKKGTIEILHSGLLPPMDMNVKQSVFAFAYSD
                     ASGVPAQSVLVEDVSDPETRVMFEDKEPKLENNQWRGITRLYSGDDPALHWLGHLGDT
                     MRVFRFTIVKPDGQKVVLHQAWSAPGWMKSSIRNALGLPPL"
BASE COUNT          191 a          411 c          304 g          215 t
ORIGIN      
        1 gcacgctgga ttgtgccgca actttccggc agtcgccggg tcttcctgtc gtgcgcattt
       61 tgaaaatcgt tctatcgatc ctcgtcgcca tcgcggcggt gatcggaggt ttgttcaccg
      121 tcgcagtcgt cgtcgtcgcg agcctcgccg tgctgctcac gcgtcgattc ctcgcgcgga
      181 ctcgcccccg cgtttccact ccgtcagcgc cggctcaggt gcgccgcacg ccagcccgcg
      241 cgcccgacgc gatcgaaatc acggcaaccg aagttcccaa cgaggaaaac acggcgtccc
      301 gagagaaaac gcccgcgccg tagttttctc gccacgcccg cgggctcgcg aacgtccgct
      361 cccttttctc ctcgctcgcc gcgcctcctt cgcgcggctt tttgccgctt acagcggcgg
      421 caagcccagc gcgtttcgga tcgagctctt catccagccc ggcgccgacc aagcttggtg
      481 cagcaccacc ttttggccgt cgggcttcac gatcgtgaaa cggaaaacgc gcatcgtgtc
      541 gccgaggtgg cccagccagt gcaacgccgg atcgtcgccg gaatagaggc gcgtgatacc
      601 ccgccactgg ttgttctcca gcttcggttc cttgtcctcg aacatcaccc gcgtttcggg
      661 atcggacaca tcctcgacca ggaccgattg cgccggcacg ccgctcgcgt cggaatacgc
      721 gaacgcaaac accgactgct tcacgttcat atccatcggc ggcagcaatc cactgtgcag
      781 gatctcgatc gtcccttttt tcgcgttcgc cggcttccct ttttccaacg gaaattccag
      841 gcgctggccc gtcccgatcg ttgtgacgta ggtatactgc ccgctggcgc aacccacgac
      901 cgtcgcgagc gccaacaaac atcccgccgc cagcatgcct gccttcaaaa cgttgcgctc
      961 tccgccgcgc agccggacac gagcacgata acagccagtc gatttgttcg tcataatggg
     1021 aaaaacgaat cgggcacacg cgcacccgaa cgcgcccatc gtccggcacc gcgtggcgcg
     1081 gcgtcaagat tcgcgtaacc cacgccggcc tcacaccgct c
//
LOCUS       DASPLT010000065         1010 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_1241, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000065 DASPLT010000000
VERSION     DASPLT010000065.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1010)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1010)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1010
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1241"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..916)
                     /locus_tag="VEA63_00695"
     CDS             complement(<1..916)
                     /locus_tag="VEA63_00695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD82523.1"
                     /translation="MNFLFVNYGDFTSNSLNHIGAFANRLTLLGHACVVAVPDNPGTI
                     TAVHQPLFTPVTFERALDREPIFPDRRGADVIHAWTPRENVREFTLAYLREHPNAALI
                     VHLEDNEAFLIESYAHEPLEKLRTLSDEELATRLSPRLSHPVRFKNFLRLAHAATYIT
                     DRLREFIPDGKPAHRLLPGIDAALYQPQTPDLSLREKIGVRSDEKLLVFTGSTTFANL
                     ADIRALLLAVRQINDRGTPCKLVRTGINPPELAAELAAIAGDHVIDLGFLEKSKLAPL
                     LALADVLVQPGTADDFNDYRLPSKIPEFL"
BASE COUNT          183 a          229 c          410 g          188 t
ORIGIN      
        1 agagaaactc cgggatcttc gacgggagac ggtagtcgtt gaaatcgtcg gcggtgccgg
       61 gttgcacgag gacgtcggcg agcgcgagca gcggggcgag tttcgatttt tcgaggaagc
      121 cgagatcgat gacgtggtcg ccggctatgg cggcgagttc ggcggcgagt tcgggcgggt
      181 tgatgccggt gcggacgagt ttgcacggcg tgccgcgatc gttgatctgg cgaacggcga
      241 gcaggagcgc gcggatgtcg gcgagattgg cgaacgtggt gctgccggtg aacacgagca
      301 gtttctcgtc ggagcggacg ccaatttttt cgcgcaggga aaggtcgggc gtttgtggct
      361 gatagagcgc ggcgtcgatg ccggggagga gacggtgcgc gggtttgccg tctggaatga
      421 actcgcgcaa gcggtcggtg atgtaggtgg cggcgtgcgc gaggcggagg aaattcttga
      481 agcggacagg atgcgacagg cgcggcgaaa ggcgcgtggc gagctcttcg tcggagagcg
      541 tgcgaagttt ttcgagcggc tcgtgcgcgt agctttcgat gaggaaggct tcgttgtctt
      601 cgagatgaac gatcagcgcg gcgttcgggt gttcgcggag gtaagcgagc gtgaactcgc
      661 gcacgttttc gcggggagtc cacgcgtgga tgacgtcggc gccgcggcgg tcggggaaga
      721 tcggttcgcg gtcgagcgca cgttcgaagg tgaccggcgt gaaaagcggc tggtgaacgg
      781 cggtgatcgt gccggggttg tcgggcacgg cgacgacgca ggcatggccg aggagcgtga
      841 gccggttggc gaaggcgccg atgtggttca ggctgttgct ggtgaagtcg ccgtaattga
      901 caaagaggaa gttcacgcga gtccgagagg gaaagttagg gcgagtggct ggatgaattc
      961 cagaaccgat ttgtaaccaa acgatggcga catgacatcc cgctgctcac
//
LOCUS       DASPLT010000066         1170 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_267193, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000066 DASPLT010000000
VERSION     DASPLT010000066.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1170)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1170)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1170
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_267193"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1170
                     /locus_tag="VEA63_00700"
     CDS             <1..>1170
                     /locus_tag="VEA63_00700"
                     /inference="COORDINATES: protein motif:HMM:NF023149.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="malectin domain-containing carbohydrate-binding
                     protein"
                     /protein_id="HYD82524.1"
                     /translation="QTGQPETVRVTGPAGFDVSLVVIEGALYLGGVPGGGYDIDPLEA
                     NTAIKVTELSGTISPEGFADFTVTPTKTDEDAGLNYITATLIDANDVKGPASDNLIIW
                     YDPLFGGDDTQAPSTPGGLNASSVTHRSVSLSWFASTDNVGVVAYDILRDGTLVSSVV
                     GLTYTDIGLEDGESYIYSVVARDEAGNTSTAATLPVETNDLGAVVKRVNAGGPNFVDS
                     KGNTWAADNGFNTGTTSTTTNAIDRTVDDTLFKTRRVDSAASAPDLIYSFALPNGSYE
                     VQLFLTENDPPNSAAGKRVFDISLEGALAFDNFDTFASAGAPYTAVVLAATTTVLDGQ
                     LNIAFGRVTGNPQIGAIKVIALPSDETEPPTAPGTLTFTNVTASTVTVNWTASTD"
BASE COUNT          230 a          423 c          320 g          197 t
ORIGIN      
        1 gcaaaccggc cagcctgaaa cggtccgtgt aacggggccc gccggcttcg atgtctcgct
       61 cgtcgtcatc gaaggcgcgc tctatctcgg cggcgtgccc ggcggcggtt acgacatcga
      121 tccactcgag gcgaacactg cgatcaaagt cacggagctg agcggcacga tctcaccgga
      181 aggtttcgcc gacttcaccg tcaccccgac caagaccgac gaagacgcgg gcttgaacta
      241 catcaccgca accttgatcg acgcgaacga cgtcaagggt cccgcgtccg acaatctcat
      301 catctggtat gacccgttgt tcggcggcga cgatactcaa gcgccttcga ccccgggagg
      361 tttgaatgcg tcgagcgtca cccatcgcag cgtatcgctc agctggtttg cctccacgga
      421 caacgtcggc gtcgtcgcct acgacattct acgcgatggc accctcgtgt cgtccgtcgt
      481 gggattgacc tacaccgaca tcggcctcga agacggcgag tcttacatct actcggtcgt
      541 cgcgcgcgac gaagcgggca acacgtccac cgccgccacg ctgcccgtcg aaaccaacga
      601 cctcggcgcc gtcgtgaaac gcgtcaacgc cggcggcccg aacttcgtcg acagcaaggg
      661 caacacctgg gccgccgaca acggtttcaa tacgggcacg acgtcgacca ccaccaacgc
      721 gatcgatcgc acggttgacg acacgctctt caaaacccgc cgcgtcgact ccgccgcgtc
      781 cgctcccgat ttgatttact cgttcgcgct gccgaatggc agctacgagg tgcagctctt
      841 cctcacggaa aacgacccgc ccaattccgc cgccggcaaa cgcgtgttcg atatctcgct
      901 cgaaggtgct ctggcgttcg acaacttcga cacgttcgct tccgccggcg cgccttacac
      961 cgccgtcgtg cttgccgcga ccacgaccgt cctcgacggg cagttgaaca tcgccttcgg
     1021 acgcgtcacc ggaaatccgc agatcggcgc gatcaaagtg atcgccttgc cgtccgacga
     1081 aaccgagccg cccaccgcgc ccggcacgct cacgttcacc aacgtcaccg cgagcaccgt
     1141 cacggtgaac tggaccgctt cgaccgacaa
//
LOCUS       DASPLT010000067         1023 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_1269, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000067 DASPLT010000000
VERSION     DASPLT010000067.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1023)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1023)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1023
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1269"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1023
                     /gene="purD"
                     /locus_tag="VEA63_00705"
     CDS             <1..>1023
                     /gene="purD"
                     /locus_tag="VEA63_00705"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009512835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HYD82525.1"
                     /translation="AAPRLFCAPGNPGIGELATCAPIASDDVPALVAFAQQQQIEFVI
                     VGPEVPLALGLVDELAKVGIPAYGPKADGARLEASKIYTKEILQKYKIPTAPAAFFDQ
                     PDAAIAYIRARPAPIVVKADGLAAGKGVVVAQNHAEAEAAVRDLLSLPGQSKILIEDC
                     LVGEETSILVVVSGRDYVILPTSQDHKRVGDGDAGPNTGGMGTYSPAEVVTPALLARI
                     DREIVRPSVDAIADEGIHFVGTLFIGIMLTAEGPSVLEYNTRFGDPETQVVLPRIETD
                     LLELLWAAAQGQLSSVPLKIKSAHALCVVIAARGYPGSYNKGDIITFPRVLPTSVSIL
                     HAGTAKN"
BASE COUNT          191 a          403 c          259 g          170 t
ORIGIN      
        1 gccgcgcccc gcctgttttg cgctcccgga aatcccggca tcggcgaact cgccacctgc
       61 gcgccgatcg ccagcgacga cgttcccgcc ctcgtcgcct tcgcgcagca gcaacaaatc
      121 gaattcgtca tcgtcggccc cgaagtcccg ctcgctctcg gcctcgtcga cgagctcgca
      181 aaagtcggca tccccgccta cggcccgaaa gccgacggcg cccggctcga agcttcgaaa
      241 atctacacga aggaaatcct tcaaaaatat aaaatcccca ccgcccccgc cgcgttcttc
      301 gaccagcccg acgccgccat cgcctacatc cgcgcgcgcc ctgcgccgat cgtcgtgaaa
      361 gccgatggac ttgccgccgg caaaggcgtc gtcgtcgctc aaaaccacgc cgaagccgag
      421 gccgccgtcc gcgatctcct ctcgcttccc ggccagtcga aaatcctcat cgaagactgc
      481 ctcgttggcg aagaaacctc gatcctcgtc gtcgtgtccg gtcgcgatta cgtgattctc
      541 cccacgtcgc aggaccacaa acgcgtcggc gacggcgacg ccggtccgaa caccggcggc
      601 atgggcacct actcgcccgc cgaagtcgtc acgcccgcgc tcctcgcgcg catcgatcgc
      661 gaaatcgttc gcccgtccgt cgacgccatc gccgacgaag gtatccactt tgtgggcacg
      721 ctcttcatcg gcatcatgct caccgccgaa ggacccagcg tgctcgaata caacacgcgc
      781 ttcggcgatc ccgaaacgca ggtcgtcctc ccgcgcatcg aaaccgatct cctcgaactt
      841 ctctgggccg ccgcgcaagg ccagttgtcc agcgtgccgc tcaagatcaa atccgcgcac
      901 gccctctgcg tcgttatcgc cgcccgcggt tatccgggtt cctacaacaa aggcgacatc
      961 atcacctttc cccgcgtgct gccgacctcc gtgtcgatcc tccacgcggg caccgcaaaa
     1021 aac
//
LOCUS       DASPLT010000068         1126 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_1279, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000068 DASPLT010000000
VERSION     DASPLT010000068.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1126)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1126)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1126
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1279"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..703)
                     /gene="lgt"
                     /locus_tag="VEA63_00710"
     CDS             complement(<1..703)
                     /gene="lgt"
                     /locus_tag="VEA63_00710"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HYD82526.1"
                     /translation="MHVVTSFALLLGYWTHDLSPFLVRFSENFGIRYYGLAYLLGFAA
                     AAWLLHLYWRAGRSQLPSAKISDLIIAVVIGVMVGGRLGSFLLYHPEQLLSDPLSFFR
                     VWEGGMASHGGMAGAAVALFWFSRSQRLPFFHLGDLIVSTAPAGLLFGRLANFINGEL
                     WGKPADVPWAVIFENTGGGPLPRHPSQLYEAALEGVLLLAIMQWRVWRSDVVKKSPGR
                     LSGEFFILYAVLRAIG"
     gene            870..>1126
                     /locus_tag="VEA63_00715"
     CDS             870..>1126
                     /locus_tag="VEA63_00715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HYD82527.1"
                     /translation="MSELKRTPLRDFHAAHGGRLVDFAGWEMPVQYRSILEEHKTVRR
                     AAGLFDVSHMGEVDVRGPDAAVFLNGLVTNDVAKLFPGRVL"
BASE COUNT          247 a          352 c          345 g          182 t
ORIGIN      
        1 cgccgatcgc gcgcaacact gcatagagga tgaaaaattc tccgctcaaa cggccggggg
       61 attttttcac cacatcgctt cgccagacgc gccattgcat aattgcgagc aggagcacgc
      121 cctcgagcgc cgcttcgtag agctgcgaag gatgccgcgg caacggaccg ccgccggtgt
      181 tttcgaagat gacggcccac ggcacatcgg cgggtttgcc ccaaagttcg ccgttgatga
      241 agttcgccag ccggccgaac aacaaacccg cgggcgccgt cgacacgatc aagtcgccga
      301 ggtgaaagaa cggcagccgt tgcgaacgac taaaccagaa cagcgcgacc gcggcgcccg
      361 ccatcccgcc atgactcgcc atgccgccct cccacacgcg aaagaagctc agggggtcgc
      421 tcaacagctg ctccggatga tacaataaaa acgagccgag ccgtccgccg accatcacgc
      481 cgatgacgac cgcgatgatc agatcggata tcttcgccga cggaagctgc gaacgtcccg
      541 cgcgccaata caggtgcagc agccacgccg ctgcggcgaa cccgagcaga tacgccaggc
      601 cgtaataacg gatgccgaaa ttctcggaaa agcggacgag aaacgggctc aggtcgtgcg
      661 tccagtagcc gagcaaaagc gcaaaagaag tgacgacgtg catctgcgca gtccgatgac
      721 atgcgtgcgt gcggcgctca atgctattga tgcagcgggc tgggtttgcg gcgtcttcgc
      781 tgaaggcgaa gtgcgagcgc cggtggatga tcttcctcga atcaaatcat ccttgccgcg
      841 acgcgtgcgg agccgcgtag tctgtcgcca tgagcgagct caaacgcacc ccgttgcgcg
      901 attttcatgc ggcccatggc ggccggctgg tcgacttcgc cgggtgggaa atgccagtgc
      961 aataccgcag cattctcgaa gaacacaaaa ccgtgcgtcg cgcggcggga ctgttcgacg
     1021 tgagccacat gggcgaagtc gacgtgcgtg gtcccgatgc cgcggtgttt ctcaatgggc
     1081 tcgtcacgaa cgacgtcgcg aagctctttc cgggccgcgt gcttta
//
LOCUS       DASPLT010000069         1767 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_1290, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000069 DASPLT010000000
VERSION     DASPLT010000069.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1767)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1767)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1767
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1290"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(74..880)
                     /locus_tag="VEA63_00720"
     CDS             complement(74..880)
                     /locus_tag="VEA63_00720"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007362378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HYD82528.1"
                     /translation="MLIDTHTHLESFARKGVLAETLARATAAGVEMMITIGTSPDDWE
                     LYRGIAETHVDAVCYTVGLHPCSVGEEWEKAVAGIEAFWGNGRIKPVALGECGLDRFH
                     LPKEAGEAEKIFAWQRAAFAEQLAIAKRLGCPVVVHSRGAFAECVAMIDASGVDWTKV
                     VFHCFTEAEAEMAELVRRGGYGSLTGIVTYKSAENVRAAAKAQGLARTMVETDAPYLT
                     PMPHRGKPNEPAFVRHTAEFCAREVFGVSEEEFAAAATANARRFFGLTSG"
     gene            complement(994..>1767)
                     /locus_tag="VEA63_00725"
     CDS             complement(994..>1767)
                     /locus_tag="VEA63_00725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009514037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HYD82529.1"
                     /translation="VRMTTRFKEDEPLDSLFSAIHETGHGLYEQGLPWEHLGTALGMN
                     AGMGAHESQSRLWENQVARSRGFWRFFEPRFRELFPEQMRGVTSGELYLAINAVEPTL
                     IRVDADEVTYNLHIILRFEIERALFAGELGVKDLPDVWREKSKALIGLEPANDREGVL
                     QDVHWSDGAFGYFPSYCLGNMMAAQLWKKVRSVRPEIEGEFGRGEFGWLLGWLRENVH
                     AHGRRYELPELVARATGEALSPRALIAYLRERYLTLYRA"
BASE COUNT          339 a          694 c          403 g          331 t
ORIGIN      
        1 tttttttgcg cccatctgcc gggccagtct caaccgccct ttttcagtct gcgattcgcg
       61 gccttttgcg tgattagccg gacgtcagcc caaaaaaccg ccgcgcattc gccgtcgccg
      121 ccgcagcgaa ttcctcttcg ctcacgccaa acacctcgcg cgcgcaaaac tccgccgtgt
      181 gccgcacgaa tgccggctcg ttcggttttc cgcgatgcgg catcggcgtg agatacggcg
      241 catccgtctc gaccatcgtc cgcgccagcc cctgcgcctt cgccgccgca cgcacgttct
      301 ctgcggattt atacgtgacg atcccggtca gcgaaccata gccgccgcgt cgaaccaact
      361 ccgccatctc cgcttcggct tcggtgaagc agtgaaacac cactttcgtc caatcgacgc
      421 cgctcgcgtc gatcatcgcc acacactccg caaacgcccc gcgcgaatgc accacgaccg
      481 gacacccgag ccgcttcgcg atcgcgagtt gctccgcaaa cgccgcgcgc tgccacgcaa
      541 aaattttctc cgcctcgccc gcctccttcg gcaagtgaaa ccgatccagc ccgcactcgc
      601 ccagcgccac cggcttgatc cgtccattgc cccaaaacgc ttcaatcccc gcgaccgcct
      661 tctcccattc ttcccccacc gagcaaggat gcagcccgac cgtatagcac accgcatcga
      721 cgtgcgtctc ggcgataccc cgataaagct cccagtcgtc cggcgacgtt ccgatcgtga
      781 tcatcatctc cacgccagcc gccgtcgcgc gcgccagcgt ctccgccagc acgcctttgc
      841 gcgcaaacga ctccagatgg gtgtgcgtat cgatcagcat gcgctgaagc tccaagatcc
      901 aagcctcgag ctccagagaa acttccaacc tcccaactgg agcttgatcc ctggctattc
      961 tctggagctt ggatcttggt ccttggagct tcctcacgct cgatagagcg tgaggtatcg
     1021 ctcccgcaaa tacgcgatca acgcccgcgg cgaaagcgcc tcgcccgtcg cgcgcgccac
     1081 caactccggc agttcatacc tgcgcccgtg cgcatgcaca ttctcccgca accagccgag
     1141 caaccacccg aactcccctc gcccaaactc cccctcgatc tccggcctca cgctgcgcac
     1201 ttttttccac aactgcgccg ccatcatgtt gcccagacaa tagctcggaa aataaccgaa
     1261 cgccccatcg ctccaatgca cgtcctgcaa caccccttcg cgatcgttcg ccggctcaag
     1321 cccgatcaac gccttcgact tctcccgcca cacatccggc agatctttca cgcccagctc
     1381 ccccgcaaac aacgctcgct cgatctcaaa gcgcaggata atgtgcagat tgtaagtcac
     1441 ttcatccgca tccacgcgaa tcaacgtcgg ctcgaccgca ttgattgcga gatacagctc
     1501 tcccgacgtc acgcctcgca tctgctccgg aaacagctcc cgaaaacgcg gctcgaaaaa
     1561 ccgccagaat ccgcggctgc gcgccacttg attctcccac agccggctct gcgattcgtg
     1621 cgcgcccatc cccgcattca tgccgagcgc cgtcccgaga tgctcccacg gcagcccttg
     1681 ttcatacagc ccgtgacccg tttcgtgaat cgcgctgaat aacgaatcga gcggttcgtc
     1741 ctccttgaac cgcgtcgtca tccgcac
//
LOCUS       DASPLT010000070         1038 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_246844, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000070 DASPLT010000000
VERSION     DASPLT010000070.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1038)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1038)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1038
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_246844"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1038)
                     /gene="secD"
                     /locus_tag="VEA63_00730"
     CDS             complement(<1..>1038)
                     /gene="secD"
                     /locus_tag="VEA63_00730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HYD82530.1"
                     /translation="PARLDFRIVHPTQSPLNTPAGETPPGYETMTLENEGRTGEVSSE
                     ELFVKRRPEMSGEAIDRAFWRPDIYGKPEIVLMFTAEGRERFAQVTRQIVQEAQAVGS
                     IGRLAIVLDGRLYSAPTVREEIDSDSAQISGSFTDREALNLANVLNNPLDLPLVVKEQ
                     YEVGPSLASDAVVSGRNAFIISTVLTIGFIVVFYTIGGLVAAIGMALNVLITLGIMAS
                     FGATLSMPGIAGIVLTLAMSVDANILIFERMREELRLGKSLSAALEAGFDKAWSAIFD
                     SNVTTFFVAVIMYALGTGPVKGFGLTLGIGIFSTMFAAVVVSKLILEAIIHTGAIKKL
                     RMFSVLQDTKFD"
BASE COUNT          212 a          342 c          294 g          190 t
ORIGIN      
        1 atcaaatttg gtgtcctgca gcacggagaa catccgcagc ttcttgatgg caccggtgtg
       61 aatgatcgct tcgaggatga gtttgctcac caccaccgct gcaaacatcg tcgagaaaat
      121 accgatgccg agcgtcaggc cgaagccctt caccggaccc gtgccgagcg catacatgat
      181 cacggcgacg aagaatgtcg tgacgttcga gtcgaagatc gccgaccatg ccttgtcgaa
      241 gcccgcttcg agcgccgcac tcagcgattt gccaagccgc aactcttcgc gcatgcgctc
      301 gaagataagg atgttcgcgt cgaccgacat cgcgagcgtc aacacgatac ccgcgatgcc
      361 cggcatcgac agcgtcgccc caaagctggc catgatgccg agcgtgatga gcacgttgag
      421 cgccatgccg atcgccgcga ccaggccgcc gatggtgtaa aacaccacga tgaaaccgat
      481 cgtcagcacc gtgctgatga taaatgcatt tcggccgctc accaccgcgt cgctcgcgag
      541 cgacgggccc acctcatact gttccttcac gacgagcgga agatcgagcg ggttgttgag
      601 cacgttcgcc aaattaagcg cttcgcgatc cgtgaagctg ccgctgatct gcgcggaatc
      661 gctgtcgatt tcttcacgca ccgtcggcgc cgaatagagc cggccgtcga gcacgatcgc
      721 gagccgcccg atcgaaccga ccgcctgcgc ttcttgcaca atctgccgcg tgacctgcgc
      781 gaaacgttcg cggccctccg cggtgaacat cagcacgatc tccggcttgc catagatatc
      841 cggccgccaa aacgcgcggt cgatcgcctc accggacatc tccggacggc gcttcacaaa
      901 aagttcttcc gagctcacct cgccggtgcg gccttcattc tcgagcgtca tcgtctcgta
      961 accgggcggc gtctcgccgg ccggcgtgtt cagcggcgat tgcgtcggat gaacgatgcg
     1021 gaaatccagt cgtgccgg
//
LOCUS       DASPLT010000071         1793 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_22034, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000071 DASPLT010000000
VERSION     DASPLT010000071.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1793)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1793)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1793
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_22034"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..630
                     /locus_tag="VEA63_00735"
     CDS             <1..630
                     /locus_tag="VEA63_00735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD82531.1"
                     /translation="IPLVSSGSRLTADTLPFPHCPGVFDWPNNVYLPIMLPELTFGDQ
                     VTLPAFYGKNCVTGLGLRNSFYFRYEQPELINLKEEFVKNLGSAKVQWTFAGNKISAE
                     FSYTVKQQVTLDKFRYVLAIAAPHSKYRLPGSLALGPQGHRCTVAKDDFQGTWRETEV
                     VSADPTYRTNYGKVHYLQYLVRDHPLIMRPGHVYRLIVNFDPEVALVDA"
     gene            698..1012
                     /locus_tag="VEA63_00740"
     CDS             698..1012
                     /locus_tag="VEA63_00740"
                     /inference="COORDINATES: protein motif:HMM:NF017451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HYD82532.1"
                     /translation="MASIERFEDIEAWQSARKLRQMVYAFTRAKPFASDFALVDQLRR
                     AAISGGSNIAEGFERGGNREFIQFLSTAKGSVAEIKDQLYCALDENYISQIQFNEAYR
                     LA"
     gene            1146..>1793
                     /gene="hisF"
                     /locus_tag="VEA63_00745"
     CDS             1146..>1793
                     /gene="hisF"
                     /locus_tag="VEA63_00745"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007363409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HYD82533.1"
                     /translation="MLSRRIIPCLDVNAGRVVKGVKFQQLRDAGDPVATARAYDAQGA
                     DELIFLDITASSDERKIMHDVVAATAEQCFMPLTVGGGLRTVTDIETMLKAGADKVSL
                     NTAAIKNPQLIADASSRFGAQCIVLAIDAKREPDGKSWRVYTHGGRNPTELDAVAWAK
                     RAVALGAGEILLTSMDRDGTGDGYDIDLTRAVSDAVTVPVIASGGAGTLDHLADVL"
BASE COUNT          366 a          633 c          472 g          322 t
ORIGIN      
        1 atcccgctcg tgagttccgg cagccggctc accgccgaca cgctgccatt tccccactgc
       61 ccgggcgtct tcgattggcc gaacaatgtt tacctgccaa tcatgctccc cgagctcacg
      121 ttcggcgatc aagtcacgct gcccgcgttc tacggcaaaa actgcgtcac cggcctcggc
      181 ctgcgcaaca gcttctactt ccgctacgaa cagccggagc tcatcaatct caaggaagag
      241 ttcgtcaaga acctcggcag cgcgaaggtc cagtggacct tcgccggcaa caaaatcagc
      301 gccgagtttt cctacacggt gaagcagcag gtcacgctgg ataaattccg ctacgtcctc
      361 gcgatcgccg caccgcattc gaagtatcgt ctccccggct cgctcgcgct cggcccgcaa
      421 ggccatcgct gcaccgtcgc gaaagacgat tttcagggca cgtggcgcga gacggaagtc
      481 gtcagcgccg atccgaccta tcgcacgaat tacggcaagg tgcattacct gcagtatctc
      541 gtccgcgatc acccgctgat catgcgcccc ggccacgtct accgcctcat cgtcaacttc
      601 gaccctgaag tcgcgctcgt tgacgcctga ggcatcaacg agccggtttg gagttttggg
      661 ttttgtgttt tgggttcggc gcgccgaacc tgccggtatg gccagcatcg aacggttcga
      721 ggatatcgaa gcgtggcagt ccgcgcgcaa gcttcggcag atggtttacg cgtttacccg
      781 cgccaaaccc ttcgcgtcgg acttcgccct cgtggatcag cttcgccgtg cggcgatttc
      841 cggcggctca aacatcgccg aaggcttcga gcggggcggc aatcgcgagt tcatccaatt
      901 tctctccacc gcaaaaggct cggtcgccga aatcaaagat cagctctact gcgcgctcga
      961 cgaaaactat attagtcaga tccagttcaa cgaagcctac cgcctcgcct aaagcacctc
     1021 gcgcctcatc ggcggattta tgacttatct ccgcaaagcc gaactcatgg gccacaagtt
     1081 cgacacgcgc tcaacgcctg cgaactcaaa acccaaaact caaaacccta aaccaatttc
     1141 cggccgtgct ctcgcgtaga attataccgt gtttggacgt caacgccgga cgcgtcgtca
     1201 aaggcgtgaa gttccagcaa ctccgcgacg ccggcgatcc tgtcgccacc gcgcgcgcct
     1261 acgacgcgca aggcgcagac gagctcatct tcctcgacat caccgcctcg agcgacgagc
     1321 gcaaaatcat gcacgacgtc gtagccgcga ccgccgagca atgcttcatg ccgctcaccg
     1381 tcggcggcgg actccgcacc gttaccgaca tcgaaacgat gctcaaagcc ggcgccgaca
     1441 aggtctccct caacaccgcc gcgatcaaaa acccgcagct catcgccgac gcctccagcc
     1501 gcttcggcgc gcaatgcatc gtcctcgcga tcgacgctaa gcgcgaaccc gacggcaaat
     1561 cttggcgcgt ctacacgcac ggcggccgca atcccaccga actcgacgcc gtggcctggg
     1621 cgaaacgcgc cgtcgcactt ggcgccggcg aaatcctgct cacctcgatg gatcgcgacg
     1681 gcactggcga cggttacgac atcgacctca cgcgcgccgt cagcgacgcc gtgaccgtcc
     1741 ccgtgatcgc cagcggcggt gccggcacac tcgatcacct cgcggacgtc ctt
//
LOCUS       DASPLT010000072         1047 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_185284, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000072 DASPLT010000000
VERSION     DASPLT010000072.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1047)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1047)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1047
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_185284"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(172..>1047)
                     /locus_tag="VEA63_00750"
     CDS             complement(172..>1047)
                     /locus_tag="VEA63_00750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD82534.1"
                     /translation="CTIEAGDDAIAFSPCADGFGSGVAENITVTNCVLHARSAAIRIG
                     WGQHDFRNLLFSNIVIRDSNRGILIQLRQGERVENVVFSNIVIETRLYRGKWWGKGEP
                     IHISALAERADETRQRTLRNVSFNNVTATGDHGVVLYADDRSTLEDITFSGVRLRVVP
                     SPLQVSFGGNFDLRPHWDPSYQVFRHDIAAVFASGVKGLRLRDVTVTWAEGLPDFFRH
                     AVEIERFTDVTIDDFRGRQAQVERSDHDAAIVLRDGCDAVVRRAKLASGGRHLTAIAG
                     VRDLTIEPSLSCSSR"
BASE COUNT          192 a          342 c          331 g          182 t
ORIGIN      
        1 gctgcggatc cgcgtcatag tccggcggca gataccggag gaattggaac tggtgggtga
       61 tcacgaggtt ggcccggtgc ggtgtctgtc gaaccggctc cgccaccgcc gacggcccag
      121 gggacagcag gatcgtgaag ctcatcaccg caagtacgac cggacttcgt ttcatcgaga
      181 ggaacacgag agcgacggct cgatcgtgag gtcgcggacg ccagctatgg cggtcaggtg
      241 ccgcccaccc gacgcaagct tcgcccggcg cacgaccgcg tcgcatccgt cgcgcaggac
      301 gatcgccgcg tcgtggtccg accgctccac ctgcgcctgg cggccgcgaa aatcgtcgat
      361 cgtgacgtcg gtgaagcgtt cgatctcgac tgcgtggcgg aagaagtccg gcaggccctc
      421 ggcccatgtc acggtgacgt cgcgcagcct cagtcccttc acgccgctcg cgaacacggc
      481 cgcaatatcg tgccgaaaca cctggtaact cggatcccaa tgcggacgca gatcgaagtt
      541 tccaccgaag ctcacctgca acggactcgg cacgaccctc aggcgcactc ctgagaaagt
      601 gatgtcttcc aacgtgctcc ggtcatcggc gtaaaggacc acgccatgat cgccggtggc
      661 cgtgacgttg ttgaacgaga cgttccgcag ggtgcgctgg cgcgtctcgt ccgcgcgctc
      721 ggccagcgcc gagatgtgga tcggctcgcc tttgccccac cattttccgc ggtaaaggcg
      781 cgtctcgatc acgatgtttg agaagacgac attctccacc cgctcgccct gccggagctg
      841 gatcaggatg ccgcggtttg agtcgcggat gacgatgttg ctgaacagca ggtttcgaaa
      901 atcatgctgg ccccagccga tccggatggc ggccgagcgg gcatgcagca cgcagttggt
      961 caccgtgatg ttttcggcca cgccacttcc aaaaccgtcc gcgcacgggc tgaaggcgat
     1021 cgcatcatcg ccggcctcga tcgtgca
//
LOCUS       DASPLT010000073         5181 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_22122, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000073 DASPLT010000000
VERSION     DASPLT010000073.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 5181)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5181)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5181
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_22122"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..458
                     /locus_tag="VEA63_00755"
     CDS             <1..458
                     /locus_tag="VEA63_00755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD82535.1"
                     /translation="FRTEHTLFVADAQIELSYCGPAHTDTDIAVRFVEADVLHLGDTY
                     FNNHYPLIDYSGGGSINGLIDATERHLSWCSSRTLVIPGHGEVGNRESLASYHRMLVV
                     CRDRIRAAKQKGLSLAEVISERPTADYDAEFGGFLIKPETFVGLVYAGV"
     gene            472..1392
                     /locus_tag="VEA63_00760"
     CDS             472..1392
                     /locus_tag="VEA63_00760"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HYD82536.1"
                     /translation="MKIASCFSPRALVVSAVPVPGSAAAAVESAVTAPTQFVAGKDGR
                     QIAYRSVGTGPALVLCNRFRGTLDTWDPAFIDGLAAHFRVITFNFRGIGRSSGEAPAK
                     IMEMADDAHDLITGLGLKKVALGGWSMGGMAAQVLATKHPELISHLILIGTGPAGKNA
                     HDMEPIFLDHAHKPVNDLADEYVLFFEPASEVSRRAADRSHERIAQRKNDLDIRVPPE
                     AWPRLHQAGAEFRADVHGSRAMLKETRLPILILHGDHDIVFPVQNWHALNRGMPTAQL
                     IVFPHAGHGPQHQFVDASVSYITAFLQSSE"
     gene            1404..2186
                     /locus_tag="VEA63_00765"
     CDS             1404..2186
                     /locus_tag="VEA63_00765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD82537.1"
                     /translation="MNITSSLRKLTVVGRSIALLSIALGLAASASPLAACELKLNHDP
                     AFAESPSLKIKKISVTYREELDLMIFQMDLAGPAGKTVPKAAGQLDGAPVLAYVFPTS
                     LKASDVNMGNVEGIVALAVTSHPDFDDTPLWDEDGNGNFGDDKVIYHTHWALLVKDDR
                     VAGGLSVKEFKKEDKAVVLPPSNCGMPMYLDSPGHAVAVRGATLRVAVPAFYMRNQKS
                     FNFDGVTCYMQVNTACMSRPMLGVYEVYSVASKDLSLPYKTK"
     gene            2268..2699
                     /locus_tag="VEA63_00770"
     CDS             2268..2699
                     /locus_tag="VEA63_00770"
                     /EC_number="1.11.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012372973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA];
                     GO_process: GO:0006972 - hyperosmotic response [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0033194 - response to hydroperoxide
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HYD82538.1"
                     /translation="MKISRHSSVTWHGGIKDGRGAISTASGALKAYPYGFASRFEGVP
                     GSNPEELIAAAHASCFTMALSLLLSEAHIAITQLDTSAEVVLESVSDGFAITRIHLTL
                     KGRVPSLDQAAFEKFATKAKLGCPVSKLFKAEITLSVELLP"
     gene            2696..3529
                     /locus_tag="VEA63_00775"
     CDS             2696..3529
                     /locus_tag="VEA63_00775"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HYD82539.1"
                     /translation="MKTPSHTIVLVHGLWVTPLSWEPFKRFYENRGHRVLAPAWPGIE
                     GSVEDMRRDPSSFNGVGAGEVVDHYAKIIRALPESPIIMGHSYGGAITQLLIDRGLGA
                     AGVAIDSVPPKGILLLPLSTNLALTPALLNPRTLRGTFLFTFKQWWRVFANTLTESEA
                     RAAYHAQAIPAPGRAIFQAALANFTPNATTTVNFQNPTRAPLLFIGGEKDVIMPASLS
                     RKIFSKHRVSPRITAYKEFPGRSHYIIAEQGWEEVADYALAWAIAHPTGGRATQVSTS
                     V"
     gene            3738..4562
                     /locus_tag="VEA63_00780"
     CDS             3738..4562
                     /locus_tag="VEA63_00780"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HYD82540.1"
                     /translation="MSTFTTKDGTQIYYKDWGSGPVVTFSHGWPLSSDAWEAQMFHLA
                     SNGYRCIAHDRRGHGRSSQPWNGNDMDTYADDLNELFAALDVRDITMIGHSTGGGEVA
                     RYIGRHGTKRVKKAVLMGAVPPIMVKTANNPGGLPIDVFDGFRKAYLADRAQFFLEVA
                     SGPFFGFNRPGAKVSEGLIRSWWTQGMMSGHKNAYDCIKAFSETDFTEDLKRFDVPTL
                     IIHGEDDQIVPIDASAKRSTKLVKGSTLKIYPGGTHSLGDTSKDQLNADLLAFVRG"
     gene            complement(4834..>5181)
                     /gene="thrH"
                     /locus_tag="VEA63_00785"
     CDS             complement(4834..>5181)
                     /gene="thrH"
                     /locus_tag="VEA63_00785"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007361620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoserine
                     phosphatase/homoserine phosphotransferase ThrH"
                     /protein_id="HYD82541.1"
                     /translation="TQVIILSDTFEQFAEPFMRQLKWPTLLCHRLVVENDRVTGYQLR
                     MPDQKRAAVQALQSLNYRVIAAGDSFNDTTMLAQADHGILFHAPDNIVKQFPQFPAFN
                     DYDDLSHAISALL"
BASE COUNT         1015 a         1635 c         1534 g          997 t
ORIGIN      
        1 tgtttcgaac cgagcacacg ctcttcgtcg ccgacgcgca gatcgaactc agttactgcg
       61 gaccagccca tacggacacg gacatcgcgg tgcgcttcgt cgaggccgat gtcctccatc
      121 tcggcgacac gtatttcaat aaccactacc cgctgatcga ttactccggc ggtggaagca
      181 tcaatggatt gatcgacgcg acagagcgtc acctttcctg gtgctcgagc cgcacgctcg
      241 tgattccagg tcacggtgag gtgggcaacc gcgagtcgct cgccagctac caccggatgc
      301 tcgtcgtctg ccgcgaccgc attcgtgccg cgaagcagaa aggtttgtcg ctggccgaag
      361 tgatcagcga gcggcccacc gccgattacg acgccgagtt cggtggattt ctcatcaagc
      421 ccgaaacgtt tgtcggcctc gtctacgccg gcgtgtgaac cgctccttat catgaagatc
      481 gcatcttgtt tttctccccg cgcgttggtg gtgagcgccg tgcccgttcc cggatcggcg
      541 gccgccgcgg tcgaaagtgc agtgacggct ccgacgcaat tcgtcgccgg caaggacggc
      601 cgccagatcg cgtatcgttc ggtcggaacc ggcccggcgc ttgtgctctg caatcgattt
      661 cgcggcacgc tcgacacctg ggatccggcc ttcatcgacg ggttggccgc acacttccgg
      721 gtcatcacct tcaacttccg cggcataggc cgctcgagtg gagaggctcc ggcgaaaatc
      781 atggaaatgg cggacgacgc gcatgacctg atcaccggac tcggattgaa aaaggtcgcg
      841 ctcggcggct ggtcgatggg cggcatggcg gcccaagtgc tcgcaacgaa acatcccgag
      901 ttgatcagcc acctgattct catcggcacc gggcccgcgg gaaaaaacgc gcatgacatg
      961 gagccgatct ttttggacca cgcgcacaag ccggtaaacg acctcgccga cgaatacgtg
     1021 ttgttcttcg aacctgcgtc ggaggtaagt cgtcgtgcgg cggaccgaag ccacgaacgc
     1081 atcgcgcaga ggaaaaacga tctcgatatt cgcgtgccgc cggaggcgtg gccgcggttg
     1141 catcaggcgg gcgcggagtt tcgcgccgac gtccatggca gtcgcgcgat gctgaaggag
     1201 acccgtctgc cgattctcat tcttcacggt gatcacgaca tcgtttttcc cgtgcagaac
     1261 tggcacgcgc tgaaccgcgg aatgcccacg gcgcaattga ttgttttccc gcacgccggc
     1321 cacggaccgc agcaccagtt cgtcgacgcg agcgtgagct acatcacggc attcttgcag
     1381 tcgtccgaat gaattttccc tccatgaaca tcacctcatc cctccgtaaa ctcaccgtcg
     1441 ttgggcgctc gatcgcgctg ctctcgatcg cgcttggtct ggccgcttca gcgtccccgc
     1501 tcgccgcgtg cgagctgaag ctgaaccacg atcccgcgtt cgcggaatcg cccagcctca
     1561 aaataaaaaa gatctcggtc acctaccgcg aagagctcga cctgatgatt ttccagatgg
     1621 atctcgccgg ccccgccggc aagaccgtgc ccaaggcggc cggccagctc gatggagcgc
     1681 ccgtgctcgc ctacgtgttt cccacctcgt tgaaggcctc cgatgtcaac atgggcaatg
     1741 tcgaaggcat cgtggcgctc gccgtcactt cgcatccgga tttcgacgac acgccgctct
     1801 gggacgagga cggcaacggc aacttcggcg acgacaaggt catctatcac actcactggg
     1861 cgctgctcgt gaaagacgat cgcgtcgccg gtggtctgtc cgtgaaagaa ttcaagaagg
     1921 aggacaaggc cgtggtgctt cctccgtcca actgcggcat gccgatgtat ctcgattcgc
     1981 ccggccacgc tgtcgccgtt cgcggcgcga ctctccgcgt cgcggtgccc gccttctaca
     2041 tgcgcaacca gaaatcgttc aacttcgacg gcgtgacctg ttacatgcag gtcaacaccg
     2101 cctgcatgtc gcggcccatg ctcggtgttt acgaggtgta ttccgtcgcc tcgaaggacc
     2161 tcagcctgcc ctacaagacg aagtaagcac gtatccaaaa attcgtcacg tgctccgcaa
     2221 ccggggtggt ttcatgccgc ccttcgtatc atcgcacgtc tttcatcatg aaaatctcac
     2281 gacacagttc agtcacgtgg cacggcggca tcaaagacgg ccggggcgcc atctccaccg
     2341 cgagcggcgc gctcaaagca tatccctacg gtttcgcgag ccgcttcgaa ggcgtgcccg
     2401 gcagcaatcc cgaggagctc atcgccgcgg cgcacgcgag ttgtttcacg atggcgcttt
     2461 cgctgctgct gagcgaggcg cacatcgcga tcacgcagct cgatacctcg gccgaggtcg
     2521 tgctcgaaag cgtctccgac ggattcgcga tcacgcgaat ccatctgacg ctgaagggca
     2581 gggtgccttc gctggaccag gccgcattcg aaaaattcgc cacaaaagcg aagctgggct
     2641 gcccggtctc aaaactcttc aaggcagaaa tcacgctgtc ggtcgaactg ctcccatgaa
     2701 aactccatcg cacaccatcg ttctcgttca cggcttgtgg gtcacgcccc tgagctggga
     2761 accgttcaaa cgtttctacg aaaatcgcgg tcatcgcgtg ctcgcgccgg cctggccggg
     2821 catcgaaggc agcgtcgaag acatgcgtcg cgatccgtcg agtttcaacg gcgtcggtgc
     2881 gggcgaggtc gtcgatcact acgccaagat catccgcgcg ctgcccgagt ccccaatcat
     2941 catgggacac tcctatggcg gcgcgatcac gcagctcttg atcgatcgcg gactcggcgc
     3001 ggcgggcgtg gcgatcgatt cggttccgcc gaaaggcatc ctgctgcttc cgctttcgac
     3061 gaatctcgcg ctgacgcccg cgttgctgaa tccccgcacg ttacgcggca cctttctctt
     3121 cacgttcaaa caatggtggc gggtcttcgc caacacgctc accgaaagcg aagcgcgtgc
     3181 ggcctaccat gcgcaggcga tccctgcgcc gggccgcgcg atttttcagg cggcgcttgc
     3241 caacttcacc ccgaacgcga cgacgacggt taacttccaa aaccccacgc gcgccccgct
     3301 gctcttcatc ggcggcgaaa aggacgtcat catgccggcg tctctcagcc gaaagatttt
     3361 tagcaagcat cgcgtgtcgc cgcgcatcac ggcctacaag gagtttcccg gccgcagcca
     3421 ttacatcatc gccgagcagg gctgggagga agtcgcggac tatgcgctcg cgtgggcgat
     3481 cgcccacccg actggcggtc gggcgacgca ggtttcaacc agcgtctagt ccggccagac
     3541 gcggacgaac gcggcatgtc acgctcgaac cgcatcgtta tatccaaacc atgaatactc
     3601 cattccgcgg cctttcacgc cgcgctttcc tgatccacag cgcgacggct gccgcggccg
     3661 ccagctttcc gttcaccctt tcggctcaaa acaggccggt ctcaaaatcc accaccacat
     3721 catcatctcg acgcaccatg agcacgttca cgaccaaaga tggcacgcag atctactaca
     3781 aagactgggg ctccggcccc gtcgtcacct tcagccacgg ctggccgctt tcctccgacg
     3841 cgtgggaagc gcagatgttt cacctcgcgt cgaatggcta ccgctgcatc gcgcacgatc
     3901 gtcgcggtca cgggcgctcg agtcagccgt ggaacggcaa cgacatggac acttacgcgg
     3961 acgacctgaa cgaactcttc gccgcgctcg atgtgagaga catcacgatg atcggtcatt
     4021 ccaccggcgg cggcgaagtt gcgcgctaca tcggccgtca cggcacgaag cgcgtgaaaa
     4081 aggccgtcct tatgggcgcc gtgccgccga tcatggtgaa gaccgcgaac aatcccggcg
     4141 ggctgcccat cgacgtgttc gacggttttc gcaaagccta cctggccgac cgggcgcagt
     4201 tctttctcga ggtcgcgagc ggtccattct ttggcttcaa ccggccgggt gcgaaggtat
     4261 ctgaagggct gatacggtcg tggtggacgc agggcatgat gtccggccac aagaacgcct
     4321 acgattgcat caaggcattt tcggaaaccg atttcacgga ggatctaaag cggttcgatg
     4381 tgccgacgct gatcatccac ggcgaggacg accagattgt gccgatcgat gcctcggcga
     4441 agcggtcgac caaattggtg aaaggttcga cgctcaagat ttacccgggc gggacgcaca
     4501 gcttgggcga caccagcaag gatcagctga acgccgacct gctcgcgttt gtccgaggtt
     4561 gaaaaacgaa ggccggggcg ccgtggagaa atgcgcggcg tcacggctct tgccgcgatt
     4621 cgctcttagc cggaaaaatt cagttcaacc gcgaatggac acgaatcgac acgaatgaaa
     4681 accggacaga aatcggcggg ttgagtcgtt gtcgaaaaag gtgaccggac cacttgtcct
     4741 tcggagcact gtttctcctg agttcgtgtg cattcgggtc ccgtcgtggt tcaaccgaaa
     4801 tgttccggcg gagccgaaca ctgtttcacg cgctcagagg agggcggata tggcgtgcga
     4861 cagatcgtcg tagtcgttga acgcggggaa ttggggaaat tgtttcacga tgttgtccgg
     4921 cgcgtggaac aggattccgt gatcggcctg cgcgagcatc gtggtgtcgt tgaacgaatc
     4981 gccggcggcg atcacgcggt agttgagcga ttggagcgcc tgaacggcgg cgcgtttctg
     5041 gtcgggcatg cgcaattggt agccggtaac acggtcgttt tcgacgacga gccggtggca
     5101 gagcaaggtc ggccatttga gctggcgcat gaagggttcg gcgaactgct cgaacgtgtc
     5161 ggagaggatg atcacctgcg t
//
LOCUS       DASPLT010000074         1589 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_123868, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000074 DASPLT010000000
VERSION     DASPLT010000074.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1589)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1589)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1589
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_123868"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..506)
                     /locus_tag="VEA63_00790"
     CDS             complement(<1..506)
                     /locus_tag="VEA63_00790"
                     /inference="COORDINATES: protein motif:HMM:NF013767.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HYD82542.1"
                     /translation="MNASTPVETFLLEARDLLDQIEEIALDATQQQPVDPEAINRLFR
                     AFHTIKGSGAMFGFEAVAGFTHHVESALDQVRSGAVRVTPRLMELILAARDQIAALLE
                     DKSSADSPESEAIIAAFRDFVNASAAVNANASGVAVVAEQPAGTRETWRIRFRPPQAI
                     AASGLNPVA"
     gene            complement(511..876)
                     /locus_tag="VEA63_00795"
     CDS             complement(511..876)
                     /locus_tag="VEA63_00795"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HYD82543.1"
                     /translation="MSKTIMTVDDSPSIRQMVGFTLRQAGHQVAEAGDGREALLKLQQ
                     QPADLVVTDLNMPHMNGIELIRQLRALPQYKFVPILLLTTESQPEKKLEGKAAGATGW
                     IVKPFTPEQLVAVVNKVLR"
     gene            complement(893..1210)
                     /locus_tag="VEA63_00800"
     CDS             complement(893..1210)
                     /locus_tag="VEA63_00800"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HYD82544.1"
                     /translation="MPHETSQLTTPTTATVADASLLTVDGPLTIAVAEAWRARLVAAL
                     AGEVGLIVDLSAATEIDVFGVQLLLAARRSAAEQGRSFRVTDNGESLTRACVSAGVDR
                     ALF"
     gene            complement(1222..>1589)
                     /locus_tag="VEA63_00805"
     CDS             complement(1222..>1589)
                     /locus_tag="VEA63_00805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD82545.1"
                     /translation="SNKTESSLDQHRNATIALFNGIAASLAKIEQCSAGMSREAEFDA
                     AFCAPLERLQVELRAIAAEVEPLVRLRSGAAESPASLELLKERYTMESERKVHAAALG
                     GTPPAPTVAAVPVDNVELF"
BASE COUNT          277 a          535 c          492 g          285 t
ORIGIN      
        1 gcgaccgggt tcagaccgct cgcggcgata gcctgcggcg gacgaaaccg gatccgccac
       61 gtctctcgcg ttcccgccgg ttgttccgcc accaccgcca cgcccgacgc gttcgcgttc
      121 accgcagcac tcgcattcac aaaatcgcga aacgccgcga tgatcgcttc cgattccggc
      181 gagtccgcgc tggatttgtc ctccaacagc gccgcgatct ggtcgcgcgc cgcgagaatc
      241 aactccatca aacgcggcgt cacacgcacg gcgccgctgc gcacctgatc gagcgcgctt
      301 tcaacgtgat gagtgaaacc ggccaccgcc tcgaagccga acatcgcgcc ggagcccttg
      361 atcgtgtgaa acgcgcggaa gagccggttg atcgcctcgg gatcgacggg ctgctgctgc
      421 gtcgcatcga gtgcgatctc ctcgatttga tcgagcaaat cgcgagcttc gagcaggaag
      481 gtttcgaccg gtgtggacgc gttcatcggt tcagcgcagg actttattca ccaccgcgac
      541 gagttgctcg ggcgtgaacg gcttcacgat ccaaccggtc gcgccggcgg ccttgccctc
      601 gagtttcttc tccggctgcg actcggtcgt gagcagcagg atcgggacaa acttgtattg
      661 cggcagcgcg cgaagctgac ggatcaactc gatgccgttc atgtgcggca tgttcaaatc
      721 ggtgacgacg agatccgccg gctgctgctg caacttcagc agcgcctcgc ggccgtctcc
      781 cgcttccgcc acctgatgcc cggcctggcg gagcgtgaat ccgaccattt ggcggatgct
      841 cggagagtcg tcgactgtca tgatggtctt gctcatggaa atacgttcct gctcagaaca
      901 gcgcgcgatc gacgccggcg gaaacgcacg cccgcgtcag cgattcgccg ttgtcggtca
      961 ctcgaaacga gcgcccttgc tccgccgcac tgcgacgcgc ggcgagcagt agttgaacgc
     1021 cgaatacgtc gatctccgtc gcggccgaga ggtcgacaat cagcccaacc tcgccggcca
     1081 acgccgcgac gaggcgcgcg cgccaggctt ccgcaacggc aatggtcaac ggtccgtcga
     1141 ccgtcagcag cgacgcgtcg gccacggtgg ccgttgtcgg cgtggtgagt tgagaagttt
     1201 cgtgaggcat cgtggtgacg gttagaaaag ttcgacgttg tccaccggca ccgcagccac
     1261 ggtcggggcc ggcggcgtcc cgcccaaagc ggcggcatgg actttgcgct ccgactccat
     1321 cgtgtagcgc tccttcagca gctcgagtga tgccggcgat tccgccgcac ccgatcgcag
     1381 gcgcaccagc ggttccacct ccgccgcgat ggcgcgcaac tccacctgca gccgttcgag
     1441 cggcgcgcag aacgcagcgt cgaactcggc ctcacgcgac attcctgccg aacactgctc
     1501 gatcttagcc agcgacgccg cgatgccgtt gaacagcgcg atcgttgcgt tgcgatgttg
     1561 atcgagactg gactccgttt tgttcgagg
//
LOCUS       DASPLT010000075         1768 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_62522, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000075 DASPLT010000000
VERSION     DASPLT010000075.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1768)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1768)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1768
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_62522"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..182
                     /locus_tag="VEA63_00810"
     CDS             <1..182
                     /locus_tag="VEA63_00810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD82546.1"
                     /translation="SKLPASAERIVFGGADANVANTDGAPPAVVPRTDRLPLSPNFNY
                     EAPANSLTILRFAAP"
     gene            632..>1768
                     /locus_tag="VEA63_00815"
     CDS             632..>1768
                     /locus_tag="VEA63_00815"
                     /inference="COORDINATES: protein motif:HMM:NF012258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0007156 - homophilic cell adhesion via
                     plasma membrane adhesion molecules [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cadherin repeat domain-containing protein"
                     /protein_id="HYD82547.1"
                     /translation="MISPALGFGALDRVVVVQAETGLLGSDYVTASADGATYVTAGTD
                     FAATAAPGTASKVASYTVTFPQPGTYHLYIRLRIGPAGGSDDSFYYGTGFGVKGVTDA
                     AGWTLVNNITGIGYTAPTETILNAGTAGMNVWKWLKLSTNNFGEAGAVFVVPAGALTQ
                     TFQFGTRENGLDVDQFAFALAGSEYLVAHFSNTRPVIDGGQIFTVAEGADNGTVIGTV
                     LATDPDPATVLKNWTIVGGAGAAVFSIDADTGVLSLDDGAGLNSEVTPSYDLSLTVDD
                     AVSTSGAQMVTVNVEKPQSILVSGPLAGRYGLGQTLSFTVLLGRNMIVDTTAGVPTLS
                     VVIGDDARAASYVSGSGTNRLVFSYAVQSGDEDSDGIAVASPIEL"
BASE COUNT          311 a          520 c          561 g          376 t
ORIGIN      
        1 cgtcgaagct gcccgcgagt gccgagcgga tcgtcttcgg cggcgccgac gccaacgtcg
       61 cgaacaccga tggagctccg ccagcggtcg tgccgcgcac cgaccgcctt cctttgtcgc
      121 caaatttcaa ctacgaggcg ccggcgaatt cgctcacgat cctgcgcttc gccgctccgt
      181 gacgatgagt tgggctcgag tgtggcgacc gcgaacggcc gcagacgctg ccgatgtctc
      241 tggaaccgcg agcgtgacac accgcggcgc tcgtcatttg gtcgccgagt agtcgaaact
      301 cggtgaccgg gtctgcatcg gatcagcggc tccatacgtt ggagaatggc atgatgcgat
      361 ttggttaatt ggatgaggag ttcccgcgag cgacgccact ctccttggtg actaaagtgg
      421 tatgactttg gtaagggcgt gattagggct ttttacatct tgtcgcgccg gacgctgtgc
      481 gcgatataac cggcgaaagc ggcgcggttg tcggtgcgcg gcgtggtgct tcgcgtctac
      541 ttccgctgcc agagctgttc gggggctcca atcccgttca taccccacta cccctatgta
      601 tcatcgacta aagtcgttgt ttcttgcttt gttgatttct ccggcgctgg gctttggcgc
      661 actggatcga gtcgtggttg tgcaggccga gacgggcctg ctcggttcag actacgtaac
      721 tgcaagcgcc gacggtgcaa cctacgtcac ggcgggcaca gatttcgcgg cgaccgcggc
      781 gccgggcacg gcaagcaagg tcgcgagtta tactgttacg tttccacagc ccggcaccta
      841 tcatctttat atccgattgc ggatcggtcc ggccggaggg agcgacgaca gcttctacta
      901 cggcactggt tttggggtta aaggcgtcac ggacgcagcg ggctggacgc tcgtcaacaa
      961 catcacgggc attggctaca cggcgccgac agaaaccatc ctgaacgcgg gcaccgccgg
     1021 aatgaacgtg tggaaatggc tcaaactttc gacgaataat ttcggcgaag ccggtgctgt
     1081 atttgtggtt cctgcggggg cattaacgca gacgttccag tttggcaccc gcgaaaacgg
     1141 cttggacgtc gatcagttcg cgttcgcgct cgccggatct gagtatctcg tcgcgcactt
     1201 ctcgaatacg cgtccggtga tcgatggcgg ccaaatcttc acggtcgcag agggcgcgga
     1261 caacggcacg gtgatcggca cggttctcgc caccgatcct gatccggcga ccgtgctgaa
     1321 aaattggacg attgtcggcg gagcgggcgc cgcggttttc tcgatcgatg ccgatacggg
     1381 agttctgtca ctcgacgacg gcgccgggct taattccgag gtgacgccca gctacgatct
     1441 ctcgctgacg gttgacgatg cggtgagcac cagcggtgcc cagatggtaa ccgtgaacgt
     1501 cgagaagccg cagtcgattc tcgtctccgg cccgctggcc ggtcgctatg gccttggaca
     1561 gacgctttcg ttcaccgtgc tgctcggccg taacatgatt gtcgatacaa cggcgggtgt
     1621 gcccacgctt tcggtcgtga tcggtgatga cgcccgcgcg gcgagctacg tctcgggtag
     1681 cggcacgaat cgcttggtgt tctcctatgc cgtgcaaagc ggtgacgaag attccgacgg
     1741 catcgcggtc gcgtccccga tcgagctc
//
LOCUS       DASPLT010000076         1387 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_82971, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000076 DASPLT010000000
VERSION     DASPLT010000076.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1387)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1387)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1387
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_82971"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            338..>1387
                     /locus_tag="VEA63_00820"
     CDS             338..>1387
                     /locus_tag="VEA63_00820"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HYD82548.1"
                     /translation="MNLTPRPTAFAGLIIGAAALLWPLSGAAQQRTAATDAARVEDEA
                     VVLSPFTVSTDKDNGYGATNAISGSRVNTAIKDIPLPIQVITNDFINDIGATDLRRSL
                     AYMSAIGLQSQNDLENNGGIFGSGTAYGPGGVNNPEGVTSNINGVQLKIRGFITNNVL
                     RDGFLRGSPSDTVNIERIEVVQGPNALLYGTGNFGGVVNYITKRPQNRAQGSTSVAYG
                     SHNFIRGTIDVTGPLLETLSYRVVGAAQEHDTHIDHQSASNFYISPSLLWRPTKTTEI
                     FVQTEFNKSEQNGFGFRALRAAQGTGATPINNDQLEATGFYWPPGADKRTWNLGGPDT
                     YNDQQQWNLEIVGTQQ"
BASE COUNT          322 a          462 c          359 g          244 t
ORIGIN      
        1 gcggctgccc aagcaacgcg aggtcaaaaa tattatgcaa caaaatacta aattcagcca
       61 cgttttacgc gcattgtgac ccggacatat cgcttaaaac tagcattata aagtagttaa
      121 gaagtaaaat tacccaaaaa acgcaacgca ccaaactatg cacatcatca ttgacacgcg
      181 tgcacaaaga ttactttcat cctcgtgtgt tccggcgtaa gtccggacat aagtctcgaa
      241 aactcctacc ccgcgtaatc cgtctccgcc gctggcgcgc cgcgctcctt ccgtcgagat
      301 ctgctgcttt cgcagggttc caacaaaccg accccacatg aacctcacac cccgtcccac
      361 ggctttcgcg ggactcatca ttggagctgc tgcgttgctc tggccacttt ccggcgccgc
      421 acagcaacgc accgccgcaa ccgacgccgc ccgcgtcgag gacgaggcgg tcgtcctttc
      481 gccgttcacc gtctccaccg acaaggacaa cggctacggc gcgacgaacg cgatctccgg
      541 ctcgcgcgtg aacaccgcga tcaaggacat cccgctgccg atccaagtta tcacgaatga
      601 ttttatcaac gacatcggcg cgaccgacct tcgccgttcg ctcgcctaca tgtcggcgat
      661 cggcctgcag tcacaaaacg atctggagaa caacggcgga atcttcggca gcgggaccgc
      721 ctacggtccc ggcggcgtga acaatcccga aggcgtcacg tccaacatca acggcgtcca
      781 actgaagatc cgcggcttca tcacgaacaa cgtcctgcgc gacggctttc ttcgcggcag
      841 cccgagcgac acggtgaaca tcgagcgcat cgaagtggtg cagggcccga atgcgctcct
      901 ttacggcacc ggcaatttcg gcggcgtggt gaactatatt accaagcgcc cgcagaatcg
      961 tgcgcagggt tccacgagcg tggcctacgg ttcgcacaat ttcatccgcg gcacgatcga
     1021 cgtcacgggc cccctcctcg aaacgctcag ctaccgcgtg gtcggcgctg cgcaggaaca
     1081 tgacacgcac atcgaccacc agagtgcgtc gaacttctat atttcgccgt cgttgctgtg
     1141 gcggcccacg aaaacgaccg agattttcgt gcagacggaa ttcaacaaat cggagcagaa
     1201 cggctttggc ttccgcgcgc tccgcgccgc acaaggcacg ggcgcgacgc cgatcaacaa
     1261 cgaccagctc gaggccactg gcttctattg gccgccgggc gccgacaagc gcacgtggaa
     1321 tctcggcggg cccgacacct acaacgacca gcagcaatgg aatctcgaga tcgtcggcac
     1381 gcagcaa
//
LOCUS       DASPLT010000077         1889 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_22197, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000077 DASPLT010000000
VERSION     DASPLT010000077.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1889)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1889)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1889
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_22197"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..494
                     /locus_tag="VEA63_00825"
     CDS             <1..494
                     /locus_tag="VEA63_00825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD82549.1"
                     /translation="FPFISGLESGPIGMIFGPDGSLFVTGSARGWGAKGGKSFHFERI
                     RWRGKLPFEPLDIRAQPDGFVVTFTEPVDPDTAANPSAYAVDAWTYLHSKAYGSEELD
                     RVTPTITQATVSPDGLSVRLVVTPLTRGHVHELKLDGIRNRRGESLVHPIGWYTLNEI
                     PAP"
     gene            complement(538..1368)
                     /locus_tag="VEA63_00830"
     CDS             complement(538..1368)
                     /locus_tag="VEA63_00830"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HYD82550.1"
                     /translation="MNGANVNRQWLLHGVLALLALLTAAPFAIMAVMSAKDLGQITLS
                     FWAWPSPFRWENYWFGLRVTVEYLGNSLAVSAAVCALTLVCAALAAHAFNRFRFPGKR
                     GLFGTLLIALMIPSALLLVPLFLVSRELGLLDTWWGLVLPQAAGALPLAILLLRSFFE
                     ELPRDLFDAAQIDGASELRVLWHVVVPLSLPVFSTVAILNLLSSWNNYIWPLLTVQSE
                     SLRTLPLGLAFLVFEQDLKFEPGKLMAAYLMASLPLLILFLFMTRPFVKGLTSGALKE
                     "
     gene            complement(1365..>1889)
                     /locus_tag="VEA63_00835"
     CDS             complement(1365..>1889)
                     /locus_tag="VEA63_00835"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HYD82551.1"
                     /translation="VVTILVWQELYGATGPVNLLLEMVGLGEHARAWLGDPTTVVPAI
                     VAIGMPFTGGVFLLIYLAGFLSIPPSLVEAARLDGAGALRLFLSVELPMLRTQIGVIA
                     LLSLLGVLQNFDNIQILTNGGPMNASMTPALYLFRSGFEFGQLGYASALGVVLLALCS
                     ALTLLVRRAGRSEK"
BASE COUNT          420 a          673 c          522 g          274 t
ORIGIN      
        1 tgtttccttt catcagcgga ctcgagagcg gcccgatcgg catgatcttc ggtcccgacg
       61 gttctctctt cgtcactggc tccgcgcgcg gttggggcgc gaaaggcgga aagtcgtttc
      121 acttcgaacg tatccgctgg cgcggaaaac tccctttcga gccgctcgat atccgcgcgc
      181 agcccgacgg ctttgtcgtc acgttcaccg aacccgtcga tcccgacacc gctgcgaatc
      241 cgtcggccta tgccgtcgac gcctggacct atctccacag caaagcctac ggcagcgaag
      301 agctcgatcg cgtgactccc acgatcaccc aagccactgt ctcacctgac ggcttgagcg
      361 tccggctcgt cgtcacgccg ctcacccgcg gccacgtcca cgaactcaaa ctcgacggca
      421 tccgcaaccg ccgtggcgag tcgctcgtgc atccgatcgg ctggtatacg ctcaacgaga
      481 tccctgcgcc atagtcggtc gaacgcgcgc ctcactcacc ttctttcgac tgccgcttca
      541 ctccttcagc gcgccgctgg tcagtccctt cacgaacggc cgcgtcatga acaaaaacaa
      601 aatcagcagc ggcagcgaag ccatgaggta agcggccatc aattttcccg gctcgaattt
      661 caagtcctgc tcaaagacga gaaacgccag cccgagcggc agcgtccgca gcgactccga
      721 ctgcaccgtc agcaacggcc agatgtagtt gttccaggac gacagcagat tgaggatcgc
      781 gacggtgctg aagaccggca gcgagagcgg cacgacaacg tgccacaaca cgcgcagctc
      841 gctcgcgccg tcgatctgcg ccgcgtcgaa caagtcgcgc ggcaactcct cgaaaaacga
      901 gcgcaacaac agaatcgcca gcggcagcgc acccgccgcc tgcggcagga ccaaacccca
      961 ccacgtgtcg agcagaccca gctcgcgcga aacaagaaac agcggcacga gcaacagcgc
     1021 gctcggaatc atcaacgcaa tcagcagcgt gccgaatagc ccgcgtttac ccggaaaccg
     1081 gaatcggttg aacgcatgcg ccgcgagcgc cgcgcacacg agcgtaagcg cgcacaccgc
     1141 cgcgctgacc gcgagactgt tgcccaaata ctccaccgtc acgcgcagcc cgaaccagta
     1201 attttcccag cgaaacggcg acggccatgc ccagaaactc agcgtgattt gaccgaggtc
     1261 cttcgccgac atcaccgcca tgatcgcaaa cggcgcggcc gtcagcagcg cgagcaacgc
     1321 gagcacgccg tgcaacagcc actggcgatt gacgttcgca ccgttcattt ctcgctcctt
     1381 cccgcacgac gcacgagcag cgtcagcgcc gagcaaagcg cgagcaacac caccccgagt
     1441 gcgctcgcgt agccgagctg tccgaactcg aagcccgaac gaaacagata aagcgcgggc
     1501 gtcatcgacg cgttcatcgg cccgccattc gtcaggatct ggatgttgtc gaaattctgc
     1561 agcaccccga gcagcgagag aagcgcgatc acgccgatct gcgtccgcag catcggcaac
     1621 tccacgctga ggaacaaccg cagcgcgccc gcaccatcga gccgcgccgc ctcgacgagc
     1681 gagggcggaa tcgagagaaa gcccgcgaga taaatcagca gaaaaacgcc accggtgaac
     1741 ggcatcccga tcgccacgat cgcaggcacc accgtcgtgg gatcgccgag ccacgcccgt
     1801 gcgtgctcgc cgagcccgac catctcgagc agcaggttga ccggacccgt cgcgccataa
     1861 agttcctgcc agacgaggat cgtgacgac
//
LOCUS       DASPLT010000078         1057 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_82978, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000078 DASPLT010000000
VERSION     DASPLT010000078.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1057)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1057)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1057
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_82978"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..987
                     /locus_tag="VEA63_00840"
     CDS             <1..987
                     /locus_tag="VEA63_00840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 27 protein"
                     /protein_id="HYD82552.1"
                     /translation="TWSLKERDENGNLVADPEKFPSGMKALGDWLHEKGFKFGIYGCA
                     GMTTCGGYPGSWGHEFQDARTFASWGVDYLKYDWCDHGTADARDAYKRMSVALRAAGR
                     PVVFSLCEWGQNKPWEWAKDIGHLWRTTGDIYDSYDGRKGWESGWKLLLNQQYTLVES
                     NGPDGLGSFAGPGHWNDPDMLEVGNEGLTFAESRAHFSFWCMLAAPLMAGNDVRNMSE
                     EIRALMTDKEAIAINQDPAGKQGFRAISEPSTNTEVWVKELSNGAWAVIAHNTGTEPS
                     DIVVEWSRLWTVRGNFNVRDVWAKREIGDTSAPYTVRIDSHDVALFRLTPTR"
BASE COUNT          213 a          268 c          383 g          193 t
ORIGIN      
        1 acgtggtcgc tcaaggagcg ggatgagaac ggcaaccttg tggcggatcc ggaaaagttt
       61 cccagcggga tgaaggcgct cggtgactgg ctgcacgaga agggattcaa gttcggcatc
      121 tacggttgcg cggggatgac gacgtgcggc ggttatccgg ggagctgggg gcacgagttt
      181 caagacgcgc gcacgttcgc gtcgtggggc gtcgattatt tgaaatacga ttggtgcgat
      241 cacggaaccg ccgacgcgcg cgatgcgtat aaacgcatga gtgtcgcgct gcgcgccgcg
      301 ggccggccgg tggtgttcag cttgtgcgaa tgggggcaga acaagccgtg ggaatgggcg
      361 aaggacatcg gtcatctttg gcggacgacg ggcgacatct acgattctta cgatggacgg
      421 aagggctggg agtcgggttg gaagctgctg ctcaaccagc aatacacatt ggtcgaaagc
      481 aacggtcccg acgggctcgg cagttttgcg gggccgggac attggaacga tccggacatg
      541 ctcgaggtgg gcaacgaggg cctgacgttc gcggagtcgc gcgcgcattt cagcttttgg
      601 tgcatgctcg ccgcgccgct gatggcgggt aacgacgtgc gaaatatgtc ggaagaaatc
      661 cgcgcgctga tgaccgacaa ggaggcgatc gcgatcaacc aggatccggc gggcaagcag
      721 ggttttcgtg cgatctcgga gccgtcgacg aacaccgaag tttgggtgaa ggaattgagc
      781 aacggcgcgt gggcggtgat cgcgcacaat accggcacgg agccgagcga tattgttgtc
      841 gaatggagcc gcctctggac cgttcgcggg aatttcaacg tgcgcgacgt ctgggcgaaa
      901 cgtgagatcg gcgacacgag cgcgccctac accgtgcgaa tcgattcgca tgacgtggcg
      961 ctgttccgtt tgactccgac gcgatgacca tcgctgacat gggaagagtt gggcgggctt
     1021 cagcccgcat cgttgaaggc ggactgaagt ccgccct
//
LOCUS       DASPLT010000079         1503 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_288400, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000079 DASPLT010000000
VERSION     DASPLT010000079.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1503)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1503)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1503
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_288400"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..565)
                     /locus_tag="VEA63_00845"
     CDS             complement(<1..565)
                     /locus_tag="VEA63_00845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HYD82553.1"
                     /translation="MNRVRRPSPSAALLLAIASLATFAGCDGGDRTPSSAPPTPPARS
                     AAIEELSYFRPQEISAGLDRPPWIAHVKPVDLDRDGQLDVIFCEAQENQVVWLRQTAR
                     GVFEEKILAADLRAPVHAETADIDGDGDLDVLVASMAVVFPHNDRIGTVFILENDGRQ
                     NFATHVVLENTSRVTDIRPADMNGDGQL"
     gene            complement(626..>1503)
                     /locus_tag="VEA63_00850"
     CDS             complement(626..>1503)
                     /locus_tag="VEA63_00850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HYD82554.1"
                     /translation="RRQNVATLAYVTDSGGEWNWKTAPAHVQFFAGASNRATQLGYRL
                     EHFWMGAPGLRQQRLSDVLYARGIAGVVFASQWSGADAAAHFEWPHFCAVKIDFLPRT
                     PAIHNVTNDQRAIMQLAMRRVMAAGYKRIGCVMPQWWDELADSAWSAGFYAEQAKLSA
                     RDRLPILSYANTPTRRDPLSPLDMSVSATKFYTWLRRHRPEVLISYGPFVRKPLADLG
                     VLIPRDFAYVDLYLETCDGATAGVRQNCDRVGEVAVDILTNQLQQHSYGLPRFATTTL
                     VEGTWFDGRTLPARN"
BASE COUNT          269 a          438 c          529 g          267 t
ORIGIN      
        1 cgagctggcc gtcgccgttc atgtccgcgg ggcggatgtc ggtgacgcgc gacgtgttct
       61 ccaaaacaac gtgcgttgcg aaattttgtc ggccatcgtt ttcgaggatg aagacggtgc
      121 cgatccggtc gttgtgcggg aagacgaccg ccatgctcgc gacgagcacg tcgagatcgc
      181 cgtccccgtc tatgtcggcg gtttcagcgt gcacgggtgc gcgaagatcg gcggcgagaa
      241 tcttttcctc gaacacgccg cgtgcggtct gccggagcca cacgacttgg ttctcctgcg
      301 cttcgcagaa aatcacgtcg agctgaccgt cgcgatcgag gtcgacgggt ttaacatgtg
      361 cgatccaagg cggacggtcg aggccggcac tgatttcctg cggacgaaaa taggacagtt
      421 cttcaatggc tgccgagcgg gcgggcggcg tcggcggagc ggacgaaggc gtgcggtcac
      481 cgccatcgca gccggcgaac gtcgccaggg atgcgatcgc gagaagaagg gcggcgctcg
      541 gcgatggccg gcgaactcgg ttcatgcggc gaagcctccg aaaacgaccg gagacttgca
      601 cctaaatttc tgcggcgccg ccacgtcaat tacgcgcggg cagcgtccgg ccgtcgaacc
      661 acgtgccttc gaccagcgtc gtagtcgcaa accgagggag cccgtagctg tgttgttgaa
      721 gctgattggt gaggatatcg acggcgactt cgccgacgcg gtcgcaattt tggcgcacgc
      781 cggcggtggc gccgtcacag gtttccaaat acaaatcgac gtaggcgaag tcgcgcggaa
      841 tcagcacgcc gagatcggcg agcggtttgc ggacgaacgg gccgtagctg atcaacactt
      901 ccggtcggtg gcgtcgcagc cacgtgtaga attttgtcgc cgacacggac atgtcgagcg
      961 gcgacaatgg atcgcggcgc gtcggcgtgt tggcatacga gaggatcggg aggcggtcgc
     1021 gggcggagag ttttgcctgt tcggcgtaga acccggcgga ccacgcggag tcggcgagtt
     1081 cgtcccacca ctgtggcatc acgcagccga tgcgtttata gccggcggcc atgacgcgtc
     1141 gcatggcgag ctgcatgatc gcgcgctggt cgttggtgac gttgtgtatc gcgggcgtgc
     1201 gcggcaggaa gtcgattttc accgcgcaga aatgcggcca ttcgaagtgc gcagcggcat
     1261 cggcgccact ccattgcgag gcgaagacca cgcctgcgat gccgcgagca tacagcacgt
     1321 cgctcaggcg ttgctggcgc aagccgggcg cgcccatcca gaaatgttcg aggcggtagc
     1381 cgagctgcgt ggcgcgattc gaggcgccgg cgaaaaactg cacgtgcgcc ggcgcggttt
     1441 tccaattcca ttcgccgccg gagtcggtca cgtaggcgag cgtggcgacg ttttgccggc
     1501 ggc
//
LOCUS       DASPLT010000080         1722 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_83002, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000080 DASPLT010000000
VERSION     DASPLT010000080.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1722)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1722)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1722
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_83002"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..475)
                     /locus_tag="VEA63_00855"
     CDS             complement(<1..475)
                     /locus_tag="VEA63_00855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD82555.1"
                     /translation="MKALLKSLLLGLIVCWLPAIAHAALGSQNNLLGKFFVSSVNGTV
                     SCVTDGRIVDLKKGDTILARGAIVETAADSNVTLVFSNGTGVYTDEKTRFEVEKFDQE
                     FFTPNNNLRVEPSNSSTIVKLATGRVVISTPRLLSGTTMVYETSHAMVGIRGDKVM"
     gene            complement(551..>1722)
                     /locus_tag="VEA63_00860"
     CDS             complement(551..>1722)
                     /locus_tag="VEA63_00860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HYD82556.1"
                     /translation="ALPLPAAALPLELPDVQSYLPSGNGESPLANVSDWLELWLNFET
                     GASVPASQPKPAGDAWIRARRETNTMPQWAGSCWYYLRFLDPKNADAIASPEALRYWG
                     VPDLYVGGAEHAVLHLLYARFWHKVLFDLGVVPQSEPFKKLFHQGIILGEDGEKMSKS
                     RGNVVSPDTIIASHGADTLRLYLMFLGPLEAMKPWNPRGIEGVHRFLQKVWRECFSVE
                     GAINPKISSDATLAEPAELTRLLHETIKKVGDDIAAMRFNTAISQMMIFTNALQKAPQ
                     VSRSSLLTFIQILAPFAPHFAEEVWARMGENPSIQRAPWPAYDPAKLEVREVKLVFQV
                     NGKHRGDALVPVGLPQDDAVALATSHERVAPFVTGKAIKRAIYVPGKILNLVVEN"
BASE COUNT          321 a          457 c          588 g          356 t
ORIGIN      
        1 tcatcacctt atcgccgcgg atgccgacca ttgcgtgcga agtttcgtaa accatcgtcg
       61 tgccgctgag cagacgaggc gtgctgatga cgacgcggcc ggtggcgagc ttgacgatgg
      121 tggaggaatt cgacggctcg acgcgaagat tgttgttcgg cgtgaagaat tcctggtcga
      181 acttttcgac ttcgaagcgg gttttctcgt ccgtgtaaac gccggtgccg ttcgagaaaa
      241 cgagggtcac gttcgaatcc gcggcggttt cgacgatcgc gccgcgggca aggatcgtgt
      301 cgcctttctt caagtcgacg atccggccgt ccgtgacgca cgacacggtg ccattcacgc
      361 tgctgacgaa aaatttcccg aggaggttgt tttgactgcc gagagccgcg tgcgcgatgg
      421 ccggaagcca gcacacaatc aatccgagta gcagggattt aaggagtgct ttcacggcga
      481 atgggaaaaa atttaggtta tacgcttgaa ctagggtgta ttacctagaa tcgattttcc
      541 cgaacgcaac ctaattttcg acgacgagat tcaggatttt cccggggaca taaatcgcgc
      601 gtttgatggc tttgccggtg acgaaaggag ccacgcgttc gtggctcgtt gcgagggcaa
      661 cggcgtcgtc ttgggggagc ccgacgggca ccagcgcgtc gccgcggtgt ttgccattca
      721 cttggaacac cagcttgacc tcacggacct cgagtttggc gggatcgtag gctggccatg
      781 gcgcgcgctg aatcgagggg ttttcaccca tacgcgccca tacttcttcg gcgaaatgtg
      841 gggcaaacgg cgcgagaatt tggataaacg tgaggagtga cgaacggctc acctgcggcg
      901 ctttttgcag cgcgttggtg aagatcatca tctgcgaaat cgcggtgttg aagcgcatcg
      961 ccgcgatgtc gtcgccgacc ttcttgatcg tctcgtggag caggcgcgtg agttcggcgg
     1021 gctcggcgag ggtggcgtcg gaggagattt tcggattgat cgcgccttcg acgctgaagc
     1081 attcgcgcca gaccttttgg agaaagcggt gcacgccctc gatgccgcgc gggttccacg
     1141 gcttcatggc ttcgagcgga ccgaggaaca tcagatagag gcgcagcgtg tcggcgccgt
     1201 gtgacgcgat gatcgtgtcg gggctgacga cgttgccgcg gctcttggac attttttcgc
     1261 cgtcctcgcc gaggatgatg ccttggtgaa agagtttttt gaacggctcg gattgcggga
     1321 cgacgccgag atcgaagagc accttgtgcc agaagcgcgc gtagagcagg tggagcacgg
     1381 cgtgttcggc gccgccgacg tagagatcgg gaacgcccca gtagcgcagc gcttcgggcg
     1441 aggcgatggc gtcggcgttt ttcggatcga ggaaacgcag gtaataccaa cacgagccgg
     1501 cccattgcgg catggtgttg gtctcgcggc gggcgcggat ccacgcgtcg ccggccggtt
     1561 tcggttgcga tgccggcacg gaggcgccgg tttcgaagtt gagccagagc tcgagccaat
     1621 cgctgacgtt ggcgagcggg ctttcgccgt tgccgctcgg cagatacgac tgcacgtcgg
     1681 gcaattcgag cggaagggcg gcggcgggca gcggcagcgc gt
//
LOCUS       DASPLT010000081         2154 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_267298, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000081 DASPLT010000000
VERSION     DASPLT010000081.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 2154)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2154)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2154
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_267298"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1413)
                     /locus_tag="VEA63_00865"
     CDS             complement(<1..1413)
                     /locus_tag="VEA63_00865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HYD82557.1"
                     /translation="MLALGAANLSQGALPPDALLTTAAQVRGLTEEEARREIPVRLRG
                     VFMGEADPEGIAFVIQDETEGIYIQGPADLVASVTRGDLLEIEGVTDPGGFAPYVVAR
                     SVTKVGRGEIPEPIQVSIDELGAGHMDAKWIEVSGIVRSVEPKSPSDNGSPPPGTRYA
                     IPSPESISQSELPKVIVTLASGNTRLSAQVCGKLNPDDYVDAVIRLRGLCFNLHNRNR
                     QFVRPFVQVPRGVEVVMEKAPPELRFSGEPRPVSGLLRFEKLTGKHGHRVHVRGVVTH
                     YQPGAVLWIRDRDHSLRVETTQDGALQLGDEVDVVGFPARGGYSAVLEDAVFRKRASQ
                     PPPAPHVLTEVSSALRKDADLVQLEARLVELRRFPDSVALTLDWHHTSIRAQLHLPEN
                     TAAPANWLPGSLVQVSGICSVITDEAGPLGGLWEPRSFQLLLRSSSDVSVIQAPPWWN
                     AERVGWVISGLLIVVLGAVAV"
     gene            complement(1633..>2154)
                     /locus_tag="VEA63_00870"
     CDS             complement(1633..>2154)
                     /locus_tag="VEA63_00870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HYD82558.1"
                     /translation="DGFADLRHWGGGTTYSSEQDTFGGRMMQDVYGPYLNAISGEGVA
                     VSELRRWRLNFVTNYTFLSGALKNVNLGGAVRWQDETAIGYYPKYNSDANIWVTDVTR
                     PIYGPAETNYDAWLGYERKLGSKYVWSIQLNVRDLFADEELIPILANPDGTIAQVRIP
                     SRTTWMLTNSIRF"
BASE COUNT          420 a          652 c          686 g          396 t
ORIGIN      
        1 cacggcgacg gcgccgagca ccacgatgag caatccggag atcacccatc caacgcgttc
       61 cgcgttccac caaggcggtg cttgaattac ggatacgtcg gaggaagacc gcagcagaag
      121 ctggaatgaa cgcggctccc ataaaccgcc cagcgggccc gcttcgtcgg taattaccga
      181 gcaaatgcca gaaacctgga cgagactccc gggcagccag tttgccggcg cagccgtatt
      241 ttcaggcagg tgcaactgcg cgcggatcga ggtgtgatgc caatccaaag tcagcgcgac
      301 gctgtcgggg aagcggcgca gctcgacgag gcgagcctcg agttgaacca gatcggcatc
      361 ttttcgcagc gcgctggaaa cttccgtcag cacgtgcggc gcgggtggcg gttgtgatgc
      421 gcgcttgcgg aaaacggcgt cctccagcac ggcactgtaa ccacctcgcg cgggaaatcc
      481 gacgacatcc acttcgtcgc cgagttgcag cgccccatct tgcgtggtct ccacgcgcag
      541 gctgtgatcg cggtcgcgaa tccagaggac ggcgccgggt tgataatggg tgacgacgcc
      601 gcgcacgtgc acgcgatgac cgtgtttgcc cgtgagtttt tcgaaccgaa gcaggcccga
      661 gacaggacgc ggttcgccgc tgaaccgtag ctcgggcgga gctttttcca tcacgacttc
      721 gactccgcga ggcacctgaa cgaacggtcg cacaaactga cggttgcggt tgtgcagatt
      781 gaaacaaagt ccgcgcaacc gaatcacggc gtcgacatag tcgtcgggat tgagctttcc
      841 gcagacttgc gcgctgaggc gagtattgcc ggaggcgagc gtgacgatga ctttcggaag
      901 ttccgattgc gagatgctct cgggcgaagg gatcgcatag cgggtgccag gcggcgggga
      961 tccattgtcg ctcggcgatt tcggttcgac gctccgcacg attcccgaaa cctcgatcca
     1021 cttcgcgtcc atgtggcccg cgccgagttc gtcgatcgag acttgtatcg gttcgggaat
     1081 ctcgccgcga ccgaccttgg tgacactgcg cgccacaacg taaggggcga agccgcctgg
     1141 atccgtcacg ccttcgatct cgagcagatc gccgcgcgtc acgctcgcca cgagatcggc
     1201 gggaccttgg atgtagatgc cttccgtttc gtcctgaatg acgaaggcga ttccctccgg
     1261 gtcggcttcg cccatgaaca cgccgcgcaa ccgcacggga atttcgcgac gcgcttcttc
     1321 ttccgtgagc ccgcgcactt gggcggcggt ggtcagtaac gcgtccggcg gcaacgcgcc
     1381 ctgggacaaa ttcgccgcac cgagcgccag caaaagggca agccgtgcgg cggatcgaga
     1441 gcggattcga gagagacgta acggaagagg agaaatcatc gcgacgttac gtgggagcga
     1501 gacgatggag cggcgacgtg cgctggcaat tcctagaatg catgacgcgg gaggcgaagc
     1561 ccgacgtcct tgtcgggctt cgtgctttgc aatctaaaac cggcccgacg ggacgtcagg
     1621 ctccacctcg aatcagaacc gaatcgagtt cgtcagcatc catgtcgtcc ggctcgggat
     1681 gcggacttgc gcgatggtgc catctggatt cgccaggatc gggatcagct cctcgtcggc
     1741 gaagaggtcg cgaacgttga gctgaatgct ccacacgtat ttcgatccga gtttccgctc
     1801 gtagccgagc cacgcgtcgt aattggtttc ggccggtccg tagatcggac gcgtgacatc
     1861 ggtcacccag atgttggcgt cggaattgta ttttggataa tagccgatcg cggtttcgtc
     1921 ctgccagcga accgcgccac cgaggttcac gttcttcagc gctccgctga gaaacgtgta
     1981 gttcgtcacg aagttgaggc gccacctgcg caactcgctg acggcgacgc cttcgccgga
     2041 gatcgcgttc agatatggcc cgtagacatc ctgcatcatg cgaccgccga aggtgtcctg
     2101 ctccgacgaa tacgtggtgc cgccgcccca gtggcgaagg tcggcgaagc cgtc
//
LOCUS       DASPLT010000082         1573 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_288454, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000082 DASPLT010000000
VERSION     DASPLT010000082.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1573)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1573)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1573
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_288454"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..755)
                     /gene="dnaN"
                     /locus_tag="VEA63_00875"
     CDS             complement(<1..755)
                     /gene="dnaN"
                     /locus_tag="VEA63_00875"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007361636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HYD82559.1"
                     /translation="MKFKINRDHFASGLAQVLNVVGSKATMPILSNVLIEAEKDHISL
                     TTTNLDLGIRCKIKADVKETGAVTLPVKRLATIVRELPNVDVQFDASPNHQVKLASGG
                     SNFRIMGIGKEEFPPLPEFGDEKAFTLEQAELTTMLKSVAYAQSTDETRYILNGVYFN
                     FKDGKLSLVATDGRRLALVAKELDVPAENAGAIILPAKTVGELLRMLDKGEKLKIAFN
                     DRRAAFQINTDKDSSGLVDSIYLYSKVVEGNYP"
     gene            complement(983..>1573)
                     /locus_tag="VEA63_00880"
     CDS             complement(983..>1573)
                     /locus_tag="VEA63_00880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007361633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HYD82560.1"
                     /translation="FGAEFDSLADVVSFGFAPALLMFFFILSPTQGITEFRNIGWFLG
                     FVYLLCAAIRLARFNVITNPLLHRGVKEANKDFVGLPVPAAAATVAALVLFLLKVIEA
                     DRTLKSGALALPFLMLLIAVLMVSTVRYPSGKNLDLQTKTKLQNFVGFLLIAGVIVLF
                     KEVAVLCVCLAYIFFGLFRHWQRARRARKNPGAVSV"
BASE COUNT          340 a          427 c          500 g          306 t
ORIGIN      
        1 ttcggataat tcccctccac gaccttggag tagaggtaga tcgagtcgac gaggccgctg
       61 gagtccttgt ccgtgttgat ctgaaaggcg gcccggcggt cgttgaacgc gatcttcagc
      121 ttctcgccct tgtcgagcat gcgcaggagc tcgcccaccg tcttggccgg caggatgatg
      181 gcgcccgcgt tctccgcggg aacgtccaac tccttggcca ccaacgcgag gcggcggccg
      241 tcggtcgcga cgagcgagag tttgccgtcc ttgaaattga aatatacgcc gttgaggatg
      301 tagcgcgttt cgtcggtcga ttgcgcgtag gcgacgctct tgagcatcgt ggtgagctcg
      361 gcctgctcga gggtaaacgc cttttcgtcg ccgaactcag gcagcggcgg aaactcttcc
      421 ttgccgatgc ccatgatgcg gaaattggag ccaccggacg cgagctttac ctggtggttc
      481 ggcgaagcgt cgaactgcac gtcgacgttg gggagctcgc ggacgatcgt cgcgaggcgc
      541 ttcacgggga gcgtgacggc gccggtttcc ttcacgtcgg ccttgatctt gcagcgaatg
      601 ccgaggtcga gattcgtcgt cgtgagcgag atgtgatctt tttccgcctc gatgagcaca
      661 ttgctgagaa tgggcatcgt ggctttcgaa ccgacgacgt tgagcacttg agccaggccg
      721 ctggcgaagt gatcgcgatt gattttgaat ttcatgaagg ggtggttccg aggcgtcttg
      781 gagaagttaa caaaggctaa aagaatagag gttcaataag gaattagagc agtattcata
      841 tggggcacca acaaccgtag taacctgatc gtcaccgtcg tgaaaccgac caaatcaggg
      901 tgtgaagaac ttttttggcg cggccaataa cctcgcggtt gttcacacgg tcgaggttgt
      961 tggtcggctg ctggcagatt gctcacacgg aaacggcacc ggggtttttg cgggcgcggc
     1021 gggcgcgctg ccaatggcgg aacagcccga agaaaatata cgcgaggcac acgcagagca
     1081 ccgcgacttc cttgaaaagc acgatgacgc cggcgatgag caggaaaccg acgaagtttt
     1141 gcagcttggt tttcgtctgc agatcgaggt tcttgccgct cggataacgc accgtgctca
     1201 ccatcaacac ggcgatcagc agcatgagaa acggcagcgc gagcgcgccg cttttcagcg
     1261 tgcggtccgc ttcgatgact ttgaggagaa acagcacaag cgcggcgacg gtcgcggcgg
     1321 ccgcgggcac gggcagacca acgaagtcct tgttcgcctc cttcacgccg cgatgcagca
     1381 gcggattcgt gatgacgttg aaacgcgcga gccggatcgc cgcacagagc agataaacga
     1441 aaccgagaaa ccagccgatg ttgcgaaact cggtgatgcc ctgcgtcggc gagagaatga
     1501 aaaagaacat cagcagcgcc ggtgcgaacc cgaaggacac gacgtcggca agcgaatcga
     1561 actcagcccc gaa
//
LOCUS       DASPLT010000083         1587 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_22260, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000083 DASPLT010000000
VERSION     DASPLT010000083.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1587)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1587)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1587
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_22260"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(286..>1587)
                     /locus_tag="VEA63_00885"
     CDS             complement(286..>1587)
                     /locus_tag="VEA63_00885"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HYD82561.1"
                     /translation="AKREAFTGALLLGDDIFRGVSRGKESRAMIRAALVGVSGYGRWH
                     LLMTMEQALLGRLRLVGATVINGVEQVAVCRRLERQGVRVFATFEEMMNALAGEIDLL
                     LLPTGIQWHAPMTLAGLRAGAHVLVEKPVAATLQDVDSILAAREATNRLVAVGFQDLH
                     VPAAHDIKRRILAGEIGTLRRAVVRGQWPRSSAYYARNVWAGRVQLGRDWVLDSPVSN
                     AFAHFLMLALFWAGRTSDAAAEPVELDAELYRAGGIDSFDTASLRLQTDEDVEILFYG
                     SHAGQEEVVPQVKLIGDAGTITWVYERTYTVDRPDRPAEILPVPDQLDTRLTVLDAVL
                     TRIERGRGFIVTPELARGHVQVINALHEYFPIHSVSASHLEEKNGGAGVFRQIRGLDE
                     TMNEVVASGLLFSAVGAPWARGSVGARCLRGYTSFGGARVW"
BASE COUNT          295 a          601 c          429 g          262 t
ORIGIN      
        1 ctccgtttag tttattgcgg ccgttctgac gtcgcggcag gcctccgtgt ctgtcagctc
       61 atcggttcgg aactgtaggg ggtgcgctcg tcgcacccgt tgcgcgcaag cgcgccaccc
      121 accccgagca tgcaggcgca gaggactgcc gctacgcaat ccacccgtcg ttttcctcga
      181 accgccgccg ctgccgcgtt cgaggacgtc gccacgaagc atctgagcgc actctgttcg
      241 ctcacccctt cgctgcgctc gaaaccaccc atcagcccgt tgcgactacc acactcgcgc
      301 gcctccgaaa gacgtatagc cgcgcaaaca acgcgcgcct accgaccccc tcgcccacgg
      361 cgcaccgacg gcgctgaaca acaagccgct cgccacgact tcgttcatcg tttcgtccaa
      421 cccgcggatc tgacgaaaca cgcccgcacc tccatttttc tcctctagat gcgacgcgct
      481 cacgctgtga atcggaaaat attcgtgcag cgcattgatc acctgaacat gcccgcgagc
      541 aagttctggc gttacgatga agccgcgccc tcgctcgatc cgcgtcagca cggcatccag
      601 cacggtcaac cgcgtatcca attgatccgg caccggcaaa atctccgccg gccggtccgg
      661 gcggtcgacc gtgtaggtgc gttcatagac ccatgtgatc gtgcccgcgt cgccgatcag
      721 cttcacctgt ggcaccacct cctcctgacc ggcatgcgag ccgtagaata aaatttcgac
      781 gtcctcgtcc gtctgcagcc gcaagctcgc cgtatcgaaa ctgtcgatac cgcccgcgcg
      841 ataaagctcc gcgtcgagct ccaccggctc cgccgccgcg tcggacgttc ggccggccca
      901 gaacagcgcc agcatgagaa aatgcgcgaa ggcattgctc accggtgaat cgaggaccca
      961 gtcgcgcccg agctgcaccc ggccggccca gacattgcgc gcataatacg ccgaggaacg
     1021 cggccactgg ccccgcacca cggcacgtcg cagcgtcccg atctcacccg cgaggatccg
     1081 gcgcttgatg tcgtgcgccg ccggaacgtg caggtcctga aatccgacgg cgacgagacg
     1141 gtttgtcgct tcgcgcgccg cgagaatgct atccacatcc tgcaacgtcg ccgccaccgg
     1201 cttttccacc agcacgtgcg cgcctgcgcg caggccggcg agcgtcatcg gcgcgtgcca
     1261 ctgaatgccc gtcggcagca gcagcaggtc gatctcgccc gcgagcgcgt tcatcatctc
     1321 ctcgaacgtg gcgaacaccc gcacgccctg ccgctcgagt cgccggcaaa ccgcgacctg
     1381 ctccaccccg ttgatcaccg tcgcgccgac cagccgcagc cggcccaaca gcgcctgttc
     1441 catcgtcatc aacagatgcc agcggccata accagaaaca cccaccagtg ccgcgcgaat
     1501 catcgcgcgt gactccttcc cgcgcgacac tccgcgaaaa atatcgtctc cgagcaggag
     1561 agctccggtg aacgcctcac gtttggc
//
LOCUS       DASPLT010000084         3773 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_22270, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000084 DASPLT010000000
VERSION     DASPLT010000084.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 3773)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3773)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3773
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_22270"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1380)
                     /locus_tag="VEA63_00890"
     CDS             complement(<1..1380)
                     /locus_tag="VEA63_00890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HYD82562.1"
                     /translation="MLSPLTHLAHRLAFASARIACSVILPGLALAPTLAMSGEAPALS
                     RSPFAPATAAPGATLFTRLDPAATGITVQNPYDDPQMWGTRYREFMGGGLGSGVAAGD
                     FDGDGRVDLYVSTKTKPGRLYRNLGDWKFADATEAAGLAENSMMSWLKGAVGSDTKTV
                     WRQGAVFADVNNDARLDLYVCRNNAPNLLYINQRDGTFTEEAEDRGLAIVDGSVVGAF
                     ADYDRDGWLDVFIVTNQVTGTEPAGRPDRLFRNHGDGRFTEVTAKAGISGATFGHSAT
                     WIDYDADGWPDLHVANDFSGPDRLYRNNGDGTFTDVLDRVVPHTPFSSMGADLADINN
                     DGHFDLFVADMATTTREKDRRGLAASRDDVVSMAARGDAAPQYMRNALLLNTGQGVFG
                     EAACWAGIDATDWTWSVRFEDFDNDGWADLHVTNGMVREPNNVDLLAQMMRALSDLQR
                     ISVIKKAPVL"
     gene            1858..>3773
                     /locus_tag="VEA63_00895"
     CDS             1858..>3773
                     /locus_tag="VEA63_00895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HYD82563.1"
                     /translation="MMPNIRTDLTTRAALTAAILLTPVLGWAQQANTPAIDADDEPVQ
                     LSPFEVRAESDSGYVTASTLAGNRLSTNLRDLGTSLSIYNQTFLNDIGATDNASLLTY
                     TLSTEVGGVYGNYSGTGGGTSPNLNASLNPSSNNRVRGLVSADNTRDLYLTSIPWEGY
                     NIDAVDLQRGPNALLFGQGSPGGVINTRTKQATFRDSNEVSLRFDEYSSARGTVDFNR
                     VLLADELALRFSAVANSSKFQQEEAFDDFHREWLAVRYEPKFLKRGNARTIIKIDGEI
                     GDGDSNRPRNTPPVDRILPWFNALDQKLYNVAWMNEGTWTIPGRGDAVQSTTGIGTNP
                     RYQPWVATNFGSNFYTGTLLTFLPGSTTPALAMGVNPVTYPAINNLGVRDGTVGGLAP
                     SQPRGIRTYRDWATNTAQPFASLVKNTHILDPNIFDFYNHMIDGDIKREWFNFHTFDA
                     SISQTFFNDKMGVDVGYHNEEYTSGGYNPVSQELHIDYNEVMPDGTNTPTSWYLDGTR
                     NPGAGRAFVQLGNGSSDNTTERSSLRATAFATHDFAKGRENWITRFLGTHTVTAMGSR
                     DTYKRFSRGWQNFAYTGAYYNHPMFQLTKDTNSRNWPDFYPTRVVYLSDSLVGKSLGQ
                     DLGLRAPGANPVPG"
BASE COUNT          751 a         1170 c         1181 g          671 t
ORIGIN      
        1 gaggacgggg gcttttttga tcacgctgat gcgctgcagg tcggagagcg cacgcatcat
       61 ctgggcgagg aggtcgacgt tgttcggttc gcgcaccatc ccgttcgtga cgtggaggtc
      121 ggcccaaccg tcgttgtcga aatcctcgaa acgcacggac caggtccagt ccgtggcgtc
      181 gatcccggcc cagcaagcgg cttcgccgaa gacgccctgt cccgtgttga ggaggagcgc
      241 gtttcgcatg tattgcggcg cggcgtcgcc gcgggcagcc atcgacacca cgtcgtcgcg
      301 ggaggcggca agtccgcgac gatccttttc gcgggtggtg gtcgccatgt cggccacgaa
      361 caaatcgaag tggccgtcgt tgttgatgtc ggcgaggtcg gcgcccatcg acgagaacgg
      421 agtgtgcggc acgacccggt cgaggacgtc ggtgaaggtg ccgtcgccat tatttcgata
      481 cagtcgatca gggccggaaa aatcgttggc gacatgcaaa tcgggccagc catcggcgtc
      541 gtagtcgatc caggtcgcgg agtggccgaa cgtcgcgccg gagatgcctg ccttggcggt
      601 gacttccgtg aagcggccgt cgccatggtt gcgaaacaga cgatccggcc gaccggcagg
      661 ctcggtgcca gtgacctggt tcgtcacgat aaagacatcg agccagccgt cccgatcgta
      721 atcggcgaag gcgcccacga cgctgccgtc cacaatcgcg aggccgcggt cttcggcctc
      781 ttccgtgaag gtgccgtcgc gctgatttat atagaggaga ttgggcgcgt tgttgcggca
      841 aacgtagaga tcgagccggg cgtcgttgtt gacgtcggca aacacggctc cctgccgcca
      901 cacggttttc gtatcggagc cgaccgcgcc tttcagccag ctcatcatcg aattttccgc
      961 gaggccggcg gcttcggtcg catcggcgaa cttccaatcg ccgagattac gatagaggcg
     1021 cccgggtttt gtttttgtgc tgacatagag atcgacgcgt ccgtcaccgt cgaaatcacc
     1081 tgcggccacg ccggagccga gtccgccgcc catgaactca cgataacgtg tgccccacat
     1141 ctggggatcg tcatagggat tttgaacggt gattcccgtg gcggcgggat cgaggcgcgt
     1201 aaagagcgtt gcgccgggcg cagcggtggc gggcgcaaac ggcgagcgac tcaaagccgg
     1261 ggcttctccc gacatcgcga gcgttggcgc gagggcaagg cccggcagga tgacggaaca
     1321 agcgatgcga gccgaggcga acgcgaggcg atgcgcgagg tgggtgagag gggaaagcac
     1381 gaagcgcgtg gggtgtgacg cggtttgcgc ggagccgcgg aagaaccggc gccgcgaaac
     1441 gctgacaggc ccgcatcacg gcggacgaca cgctcagcgc aacggggcag tggttatagg
     1501 taatagtata tccaaagata tatcgtggat gcagcgcttg gggcggatgg tgtatcgcga
     1561 ggggcggatg gcgcaaaaag agaggtaatt tggcgtaaac ggtccgtagc cggggacgag
     1621 tcctgcggct attctcactt cgaacgcggt gaccgcctac ccaagcgtgt tcttccgaca
     1681 gatcgtgtta ccccctccac tgcacgtcat gccgacttcg tggcccccaa gcgcgagctc
     1741 atgggcaagg agacggcgtg ccttgaacag ttgttcgcca gcagcctggc gcttcggtcg
     1801 cacggtttgt tcgggaggcc tacgagggga ggagcgagcc gcgtaacctc aaacaccatg
     1861 atgcccaata taagaaccga cctaaccacc cgcgccgcgc tgactgcggc gatcctgctc
     1921 acacccgttc tcgggtgggc gcagcaggca aataccccag ccatcgacgc cgacgacgag
     1981 ccggtgcaac tctcgccttt cgaagttcgg gcggagtcgg actccggcta tgtcacggcc
     2041 tcgacgctgg ccggcaatcg gctgagcacg aaccttaggg acttggggac ttcactctcc
     2101 atctataatc agacctttct caacgatatc ggtgcgacgg ataacgcctc cttgctcacc
     2161 tacacgttgt ccaccgaggt gggcggcgtt tacggcaatt actccggcac cggtggtggc
     2221 acttcgccca acctcaacgc cagcctgaat ccgtcctcga acaatcgggt tcgcggcttg
     2281 gtcagcgcgg acaacacgcg cgacctctat ttgaccagta tcccatggga gggctacaac
     2341 atcgacgcgg tggatctcca acgcgggccg aatgcgctcc tgttcggtca gggcagcccc
     2401 ggcggcgtga tcaacacccg cacgaaacag gcgaccttcc gcgattcgaa cgaagtgtcg
     2461 ttgcgcttcg acgagtatag tagcgcgcgc ggcacggtgg acttcaaccg cgtcctcctg
     2521 gctgacgaac tggcactccg cttctccgcg gtggccaaca gcagcaagtt ccagcaggag
     2581 gaggcgttcg acgacttcca ccgcgagtgg ctggcggtgc gctacgaacc gaagttcctc
     2641 aagcgcggca acgcccgcac gatcatcaag atcgacggtg aaatcggcga cggcgacagc
     2701 aaccgcccgc gcaacacgcc gccggttgac cgcatcctgc cctggttcaa cgcgctcgac
     2761 cagaagctct acaacgtcgc ttggatgaac gaaggcacct ggacaattcc gggccgtggc
     2821 gacgcggtgc aatccaccac cggcatcggc accaaccccc gctaccagcc ttgggtcgcg
     2881 acaaactttg gcagcaactt ctatacgggc acgctcctga ccttcctccc gggatcgacc
     2941 acgcctgcgc tcgcgatggg cgtcaatccc gtgacttatc cggcgatcaa taatctaggc
     3001 gtgcgcgacg gcacggtggg cggcctggcg ccttcccaac ctcggggcat tcgcacgtat
     3061 cgcgattggg cgaccaacac cgcgcagccg ttcgccagtc tggtgaagaa cacccacatc
     3121 ctggatccga acatcttcga tttctacaat cacatgatcg atggtgatat caaacgggag
     3181 tggttcaact tccacacctt cgatgcctcg atcagccaga cgttcttcaa cgacaagatg
     3241 ggtgtcgatg tcgggtatca caacgaagaa tacacgagcg gcggctacaa tcccgtctcc
     3301 caggagcttc acatcgacta caacgaggtg atgccggacg gcacgaacac gccgacgagc
     3361 tggtatctcg atggcacgcg caatccggga gcgggacgcg ccttcgtgca actcggcaac
     3421 ggtagcagcg acaatacgac cgaacgttcg agcctgcgcg cgacggcgtt tgcgacgcac
     3481 gatttcgcca agggccgcga aaactggatc acccgctttt tgggcacgca cacggtgacg
     3541 gcgatgggtt cccgcgatac ctacaagcgt ttcagccgcg gctggcagaa cttcgcttac
     3601 acgggcgcct attacaatca cccgatgttc cagctcacga aggacacgaa ttcacgcaac
     3661 tggcctgatt tctacccaac ccgggtggtt tatctcagcg acagcctcgt gggcaagtcg
     3721 ctcggtcagg acttgggcct gcgggctccg ggagccaatc ccgtccccgg tga
//
LOCUS       DASPLT010000085         1526 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_144639, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000085 DASPLT010000000
VERSION     DASPLT010000085.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1526)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1526)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1526
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_144639"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1526)
                     /locus_tag="VEA63_00900"
     CDS             complement(<1..>1526)
                     /locus_tag="VEA63_00900"
                     /inference="COORDINATES: protein motif:HMM:NF016260.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HYD82564.1"
                     /translation="ARLELVAQSSSNPEFWKKVTELTGVDLVKPEAVINLRGTWSEPR
                     GEARLSVERVAPVAGRFKTSWPAIDALEAKFVADGNGAKLDPLSVKLNGQTVRVTGRV
                     PVSPTEWRKVWKNPLMVAERGALNIQIPDAELAALAQQFPELLAPKGRLQLDLTVKPD
                     AMLEGYLRVRDGTSRPLGPLGVLQDINAEVRFNGRAAELASVTARMGGQPVMLQGRAE
                     LPVDGGPQLDLTLKGTNLPFVRRTGLLLRGDLDLTLKGSTAGRTAIGGTVRLRDSLFL
                     ADVRSLIPTGTKGSAARPPYFSIEAEPLRSWQLDVAVEGAHFLRLRTPVFNGTASINV
                     RLEGTLGNPRATGEAVIDEGRVRLPFATFEVRQGHLRLTPDQAEPQLLVMGTTRRYGY
                     DLRMELTGRVSAPELTFSSSPPLEAEQVLLLVMTGQAPNDEITTTNRQRVTRLGAFVG
                     QSLLGSFAGDSGGADRLTISSGEDISAQGRETYSIEYLLSNRWAVTGEYDEFDDYYGG
                     "
BASE COUNT          286 a          591 c          388 g          261 t
ORIGIN      
        1 atccgccgta gtaatcgtcg aactcgtcgt attcacccgt caccgcccaa cgattgctca
       61 acaggtattc gatgctgtag gtctcgcgtc cctgcgccga aatatcctcg ccgctcgaga
      121 tcgtcagccg gtccgctcca cccgagtcgc ccgcaaacga gccgagcaaa ctctgcccca
      181 caaacgcgcc cagtcgcgtc acccgctgac gattcgtcgt cgtgatttcg tcgttcggcg
      241 cctgcccggt catcaccagc agcaacacct gctccgcctc gagcggcggg ctcgacgaga
      301 aggtcagctc cggcgccgac acgcgccccg ttaactccat ccgcaaatcg tagccatatc
      361 ttcgcgtcgt gcccatcacc agcaactgcg gttccgcctg atccggcgtg agccgcaaat
      421 gcccttgtcg cacctcgaac gtcgcgaacg gcaatcgcac gcgcccttca tcgatcaccg
      481 cttcgcccgt tgcgcgcgga ttgccgagcg tgccttcgag ccgcacgttg attgacgcgg
      541 tgccattgaa gacgggcgtc cgcaaacgca ggaaatgcgc gccttccacc gcgacatcga
      601 gctgccacga ccgcaacggt tccgcctcga tcgaaaaata cggcggacgc gccgcactgc
      661 ctttcgtgcc cgtcggaatc aacgaacgca catccgcgag aaacaaactg tcgcgcaacc
      721 gcaccgttcc gccaatcgcc gttcgccccg ccgtcgagcc cttcaacgtc agatcgagat
      781 cgccgcgcag cagcaaaccc gtgcgccgca cgaacggcag attcgtgcct ttcagcgtca
      841 ggtccaattg cgggccgccg tccaccggca gctccgcgcg accttgcagc atcaccggtt
      901 gcccgcccat ccgcgccgtc accgaagcga gttcggccgc acgtccgttg aaacgcacct
      961 ccgcattaat gtcctgcaac acgcccaacg gccccagcgg acgcgaggtc ccgtcacgca
     1021 cgcgcagata accttcaagc atcgcgtccg gcttcaccgt cagatcgagc tgcaaacgcc
     1081 ccttcggagc gagcagttcg ggaaattgct gcgcgagcgc cgccagctcc gcatccggaa
     1141 tttggatatt caacgcgcct cgctccgcca ccatcaacgg attcttccac accttccgcc
     1201 actccgtcgg cgaaaccggc acgcgtccgg tcacgcgcac cgtttgcccg ttcagcttga
     1261 ccgaaagcgg atccaacttc gccccattgc catcggccac gaatttcgcc tccagcgcgt
     1321 cgatcgccgg ccagctcgtt ttaaaacgtc ccgccacggg cgccacccgc tcgacgctca
     1381 acctcgcctc gccgcgcggc tcgctccaag tcccgcgcaa attgatcacg gcctccggct
     1441 tcaccaaatc cacgcccgtc aactccgtga ccttcttcca aaattccgga ttcgacgagc
     1501 tctgcgcgac gagttcgagc cgcgcg
//
LOCUS       DASPLT010000086         2028 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_288477, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000086 DASPLT010000000
VERSION     DASPLT010000086.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 2028)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2028)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2028
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_288477"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..245)
                     /locus_tag="VEA63_00905"
     CDS             complement(<1..245)
                     /locus_tag="VEA63_00905"
                     /inference="COORDINATES: protein motif:HMM:NF024912.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD82565.1"
                     /translation="MCGIAGFLSPKQAYGREALERAGASLRHRGPDVRRWWLSDDGRA
                     GLAHARLSIIDLVTGDQPIANEDGSCRIVANGEFYDY"
     gene            386..1363
                     /locus_tag="VEA63_00910"
     CDS             386..1363
                     /locus_tag="VEA63_00910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007362737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HYD82566.1"
                     /translation="MIEVSQLTRIFRTYKKQPGFWGGVRGLFHRTFEETAAAKDISFQ
                     IAEGEFVGFLGPNGAGKTTTLKMLSGLIYPTSGTATVAGFDPTKRENAYRRIFALVLG
                     QKNQLWWDLPAIESFHLLRAIYAIPPEQFKTTLDELVDLLDVRPKLNVMVRELSLGER
                     MKMELIAALLHRPRVLFLDEPTIGLDVVSQKAVRNFLRDYNRRHRVTILLTSHYMADI
                     KELCERVIVIHKGTKIYDGALARLENSGGARKKIIKFRPVESTFPALVELAEDKASVS
                     PDGEVTLHVPVDQVVAVSQRILAAGPVADITIEDVPLEDVIAELFTSKS"
     gene            1386..>2028
                     /locus_tag="VEA63_00915"
     CDS             1386..>2028
                     /locus_tag="VEA63_00915"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HYD82567.1"
                     /translation="MKRISVIIPFYGRPDQLHTCLVALEAQTIPRDDFEVIVVDNGSP
                     HDLGALRQRFPLVRWLREERPGSFAARNRGIEIAQGRILAFTDSDCVPTPAWLHEGAT
                     LLESGPATVIGGRVAYLDKGSPELNTAERFEEEFFLLHKQKYLVEALNVSATANLLAL
                     KSAFDRVGPFDMTLLNFADGDWTQRAVRAGETLRYSEHALVHHPRRRTFREIQL"
BASE COUNT          406 a          709 c          537 g          376 t
ORIGIN      
        1 tcgtaatcgt agaactcgcc gttcgcgacg atccgacacg agccgtcctc gttcgcgatt
       61 ggctgatcgc ccgtcaccaa atcgatgata ctcagccggg catgcgcaag ccccgcacga
      121 ccgtcgtccg acaaccacca acgccggaca tccggcccgc gatgccgcag actcgctccc
      181 gcccgctcga gcgcttcacg accataagct tgtttcggcg acaaaaagcc agcgatgccg
      241 cacatgtcag aaaccggatt ttcccagcgc tacgcagatc ataaaccgcc gaacatcgtc
      301 acagacgttt cgacgacgca atcatccagt tgccgcaatc ttccgctttg cttccggcag
      361 ccaagacact tcaatgcgcc gctccatgat cgaagtctcg cagctcacgc gtatcttccg
      421 cacctacaaa aagcagcctg gcttctgggg cggcgtgcga ggcttgtttc accgcacgtt
      481 tgaggaaacc gcggcggcaa aagatatttc cttccaaatc gctgaaggcg agttcgtcgg
      541 cttcctcggg cccaacggcg cgggcaagac gaccacgcta aaaatgctct cggggctgat
      601 ctatccgacg agcggcaccg cgaccgtcgc cggcttcgat cccacgaaac gcgagaatgc
      661 ctatcgccgc atcttcgcac tcgttctcgg ccagaaaaac cagctctggt gggatctccc
      721 cgcaatcgaa tcgtttcacc tgctccgcgc gatctacgcg atccctccgg agcagttcaa
      781 aaccaccctc gacgagctcg tcgacctgct cgacgtccgc cccaagctca acgtgatggt
      841 tcgcgagctc tcgctcggcg aacgcatgaa gatggagctc atcgccgcgc tccttcaccg
      901 cccgcgcgtg ttgtttctcg acgagccgac gatcggcctc gacgtcgtct cgcaaaaagc
      961 cgtgcgcaat ttcttgcgcg actacaaccg ccgccaccgc gtcaccattc tcctcacgag
     1021 ccactacatg gccgacatca aggaactctg cgagcgcgtg atcgtgatcc acaagggcac
     1081 gaaaatctac gacggcgcgc tcgcgcgcct cgagaactcc ggcggcgcgc gcaagaagat
     1141 cattaaattt cgtcccgtgg agagcacgtt tcccgcgctc gttgagctcg ccgaggacaa
     1201 ggcaagcgtc tccccggatg gcgaagtcac gctccatgtg cccgtcgacc aggtcgtcgc
     1261 cgtctcgcag cgcatcctcg cggccggtcc cgtcgccgac atcacaatcg aagacgtgcc
     1321 actcgaagac gtgatcgcag agctcttcac gtcgaagagc tgacctgcgt tttgtgtggc
     1381 taacgatgaa gcgaatctcg gtcatcattc cgttttacgg ccggcccgat caactgcaca
     1441 cgtgtctcgt cgcgctcgaa gcgcagacaa ttccgaggga cgacttcgaa gtgatcgtgg
     1501 tggacaacgg ctcgccgcac gatctcggcg cactacgcca acgttttcct ttggtgcgct
     1561 ggcttcgcga agaacgcccc ggctcgttcg ccgcgcgcaa tcgcggcatc gaaatcgcgc
     1621 aaggccgcat cctcgccttc accgactccg actgtgtgcc gacgcccgcc tggctgcatg
     1681 aaggcgccac tctgctggaa agcggccccg ccacggtgat tggcggacgt gtcgcttacc
     1741 tcgacaaggg ctcgcccgaa ctgaacaccg ccgaacgttt cgaggaggag ttttttcttc
     1801 tccataaaca aaagtatctc gtcgaagcgc tcaacgtctc ggcgaccgcg aacctcctcg
     1861 cgctcaaatc cgccttcgac cgcgtcggcc cgttcgacat gacgctgctg aatttcgccg
     1921 acggcgattg gacgcaacgc gccgtgcgcg ccggcgaaac gctgcgctat tccgagcacg
     1981 cgctcgtcca ccatccgcgt cgccgcacgt ttcgcgaaat ccagctca
//
LOCUS       DASPLT010000087         2821 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_185420, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000087 DASPLT010000000
VERSION     DASPLT010000087.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 2821)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2821)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2821
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_185420"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..592)
                     /gene="dgt"
                     /locus_tag="VEA63_00920"
     CDS             complement(<1..592)
                     /gene="dgt"
                     /locus_tag="VEA63_00920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008832 - dGTPase activity [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dNTP triphosphohydrolase"
                     /protein_id="HYD82568.1"
                     /translation="MPNRFYNAFDVETYRGERRADYRSAFQIDRDRIIHAQAFRKLQS
                     KTQVFLSGEYDFYRTRLTHSMEVAQIGRSICHYLQTRGGPLKEDFFIDGDLVEGVCLA
                     HDLGHPPFGHNGEKALNAWAADIGGFEGNAQSLRLLTRLEPKVFDTDGRTVGLNLTRA
                     SLDASVKYPWGAQDAPVGADGVRTPKFGVYPDDLPAF"
     gene            649..1128
                     /locus_tag="VEA63_00925"
     CDS             649..1128
                     /locus_tag="VEA63_00925"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: protein motif:HMM:NF013222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HYD82569.1"
                     /translation="MTFDTPLGRCALAWHAAGLTGFSLPDAPILADDVATPPPDIAAV
                     IVRVRRHLSGEPQDFSDLRYDFTDLADFHRRVLRATLEIKSGHTRSYGEIAETIAELP
                     VASRAVGAALGANRWPLLIPCHRVVSSEGKMIGFSGPGGVKTKLRLLALEGAQLFGE"
     gene            1463..2170
                     /locus_tag="VEA63_00930"
     CDS             1463..2170
                     /locus_tag="VEA63_00930"
                     /EC_number="2.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016432 - tRNA-uridine
                     aminocarboxypropyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-uridine aminocarboxypropyltransferase"
                     /protein_id="HYD82570.1"
                     /translation="MSRETCYRCFWPKPLCWCSSIQPIAPRTKFVFLMHPKEFKQEKA
                     ATGRLAHLCLTSSEIHMGVSFENDEAVNALIRDPRHLAVLLYPGPNARNLSNSELSPV
                     ELGSRQLVVFLLDATWACARKMLRLSPTLQQLPRIMFTPSTPSRYVIKQQPQPGCLST
                     LEAVHETLTALERSGLDRYDRPEQLLTLFARMQDYQIRCAQDPSRRGYRRQPYSDPAE
                     RTAARGDSARRSRYLPI"
     gene            complement(2306..>2821)
                     /locus_tag="VEA63_00935"
     CDS             complement(2306..>2821)
                     /locus_tag="VEA63_00935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD82571.1"
                     /translation="AFDPTGAYDFLVGPTATNLPANNGSTLFEDHEIGDYDGVADIAL
                     INIQSDSGRFGGLRCANTSFFAASGSTGVSAPGVAFEGPFYVHNITAEDDANPTLVMG
                     AIGTGGIGITGGDMLQPNGRAVAVGEAGFIEMRAGQDSHGRAQPVQVNAGTFDRGGVD
                     VTATLVRPSAP"
BASE COUNT          539 a          991 c          774 g          517 t
ORIGIN      
        1 cgaacgccgg caggtcgtcc gggtagacgc cgaacttggg cgtgcggacg ccgtcggcgc
       61 cgacgggcgc gtcctgcgcg ccccacgggt acttcaccga ggcgtccagg ctggcccggg
      121 tcaggttcag gccgacggtg cggccgtcgg tgtcgaacac cttcggctcg agccgggtga
      181 gcaggcggag cgactgtgcg ttgccctcga agccgccgat gtcggccgcc caggcgttca
      241 gcgccttctc gccgttgtgg ccgaacggcg gatgccccag atcgtgcgcg agacacacgc
      301 cttccaccaa atcgccgtcg atgaaaaagt cctccttcaa cggtccgccg cgcgtctgca
      361 aatagtgaca gatcgagcgc ccgatctgcg cgacctccat cgagtgtgtc agccgcgtcc
      421 gataaaaatc atactcgccc gagagaaaca cctgcgtctt tgactgcagc tttcgaaacg
      481 cctgcgcgtg gatgatccgg tcgcgatcga tttgaaatgc gctgcgataa tccgcgcgcc
      541 gctcgccgcg gtaggtctcg acatcgaagg cgttgtagaa acggttcggc atggtttcgt
      601 ttcgacacga cagccaagcc gcatatgatg caaaccctta tgcagcgcgt gacgtttgac
      661 accccgctcg gccgctgcgc cctcgcgtgg cacgccgcgg gtctcaccgg tttcagcctt
      721 cccgacgcgc cgatcctggc cgacgacgtc gcgacgccgc cacccgacat cgccgccgtg
      781 atcgtccgcg tccgccgtca tctcagcggt gagccgcagg atttttccga cctccgctac
      841 gatttcaccg acctcgcgga tttccatcgc cgcgtgctgc gcgccacgct cgagatcaaa
      901 tccggccaca cgcgcagcta tggcgaaatc gccgaaacga tcgccgaatt acccgtcgcc
      961 agtcgcgccg tcggcgccgc gctcggagcg aaccgttggc cgctgctgat tccgtgtcac
     1021 cgcgtagttt cctccgaagg aaaaatgatc ggcttctccg gccccggcgg cgtgaaaaca
     1081 aaactccgtc tcctcgcact cgaaggcgcg caacttttcg gcgagtaaaa ccgcctcgat
     1141 tctcaaagta gggcgggctt cagcccgctt taggggacgc atgcaaaaga gaaatcacga
     1201 atcgaacgaa taaacacgaa ttcagaggag ccgaaggaaa gctttgacgc cgatcactcc
     1261 atcacccttc ggtgttccaa taaggctccg cattccagtg cgttttcatt cgtgcccatt
     1321 cgtgtccatt cgtggttcaa atgaatttcg cccttagcgt tcgcgatcaa ggcaggctaa
     1381 agcccgccct actctgctcc cgcattctcg ttcgcccacc aggaacgttg cgcgccctcc
     1441 gccggctcac ctacgctcga gcatgtctcg cgaaacctgc taccgctgct tctggcccaa
     1501 gcccctgtgc tggtgttcgt cgattcaacc gatcgcgccg cgcacgaagt tcgtgttcct
     1561 gatgcacccg aaggagttca agcaggagaa agccgcgacc ggccgcctcg cgcacctctg
     1621 cctcacgagc agcgaaatcc acatgggcgt ttcgttcgaa aacgacgaag cagtcaacgc
     1681 actcattcgc gacccgcgcc atctcgccgt cctgctctac cccggaccga atgcgcgcaa
     1741 tctctcgaac agcgaactct cacccgtcga actcggctcg cgccagctcg tcgttttcct
     1801 cctcgacgcc acgtgggcgt gcgcccgcaa aatgcttcgg ctcagtccga cgctgcagca
     1861 actcccacgg atcatgttca cgccgtcaac gccgagccgc tacgtgatca aacagcagcc
     1921 gcagcccgga tgtctttcga cgctcgaagc cgtgcacgaa acgctcaccg cgctcgaacg
     1981 ctccggtctc gatcgctacg atcgccccga gcagctgctc acgctgttcg cacgcatgca
     2041 ggattaccag atccgctgcg cgcaagatcc cagccgccgc gggtatcggc gccagcccta
     2101 cagcgatccc gccgagcgca ccgccgcgcg cggcgacagc gcccgccgca gccgctacct
     2161 tccgatctaa agcagatcaa tcaaacgata gccgcaaaga ggtgcagaag gcgcaaaaat
     2221 gacctggagg attcttctgt gctcgcgcct tttgcgcctc tttgcggcca cagattcggc
     2281 tatagaactt ttttagccgc tgtaatcaag gcgcgctcgg acgcacgagc gtcgccgtaa
     2341 catccacgcc gccgcgatcg aacgtgccgg cgttgacctg caccggttga gcgcggccgt
     2401 gcgaatcctg gccggcgcgc atctcgatga atcccgcttc gccgaccgcg accgctcgac
     2461 cattcggctg aagcatgtct ccacccgtga tcccaatccc gcccgtccca atcgcaccca
     2521 tcacaagcgt gggattcgca tcgtcctccg ccgtgatatt gtggacataa aacggtccct
     2581 caaaagcaac acctggcgcc gagacccccg tcgatccgct cgccgcaaaa aacgaggtat
     2641 tggcgcagcg cagcccgcca aacctgccgc tgtcactctg aatgttgatc aacgcgatat
     2701 ccgccacgcc gtcgtagtct ccgatctcgt gatcttcaaa cagcgtgctg ccgttgttcg
     2761 ccggcaggtt tgtggcggtt ggcccgacca gaaaatcata cgcgccggtc ggatcgaacg
     2821 c
//
LOCUS       DASPLT010000088         1761 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_288519, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000088 DASPLT010000000
VERSION     DASPLT010000088.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1761)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1761)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1761
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_288519"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..229
                     /locus_tag="VEA63_00940"
     CDS             <1..229
                     /locus_tag="VEA63_00940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HYD82572.1"
                     /translation="YAAGTVVGAVAGNVAGAVAGGAEGAVQAAKAPFTNERRIVRTWR
                     TETTTDGRTIQVPVETEVDEHGRPISSTATK"
     gene            complement(553..1161)
                     /gene="lpoB"
                     /locus_tag="VEA63_00945"
     CDS             complement(553..1161)
                     /gene="lpoB"
                     /locus_tag="VEA63_00945"
                     /inference="COORDINATES: protein motif:HMM:TIGR02722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoB"
                     /protein_id="HYD82573.1"
                     /translation="MNQIHFNKLPVSAALAVAALFFAGCETTPETRTIDAKGPEALNT
                     SSINSQDWANAADQLVQSLLSSSALERAPAQPAILAVDRVINNTTLSIDTDLLIKKIR
                     VALTQTGKIAVTNTMGLGERAVVASEAAELEEMTTGKKQKLVAPHYTLYGKLIQQTDR
                     AGSQTQNTYSFQMSLVDVKSGLTVWEEERSIAKMTKRASVGW"
     gene            complement(1177..1581)
                     /locus_tag="VEA63_00950"
     CDS             complement(1177..1581)
                     /locus_tag="VEA63_00950"
                     /inference="COORDINATES: protein motif:HMM:NF018889.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcfL family protein"
                     /protein_id="HYD82574.1"
                     /translation="MKSSLLAITATLLLAGCGTTVNTVERAQAQASPSYVADKRVVTD
                     RTLAATFHVVSINEATVSGNLLKIQATIENLKNSTRALSYKFEWVDANGMAVDSPNET
                     WKQIRVQPRETTTISTVAVTPRAVDFVLKLKE"
     gene            complement(1578..>1761)
                     /locus_tag="VEA63_00955"
     CDS             complement(1578..>1761)
                     /locus_tag="VEA63_00955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD82575.1"
                     /translation="TSLPKEFQYARILTPADRQLTLTAGTTTQTVSLVPGAVNVVYVK
                     SASSSAPLLVSQFAIK"
BASE COUNT          316 a          556 c          540 g          349 t
ORIGIN      
        1 ctacgccgca ggaacggtcg tgggtgctgt cgccggcaat gtcgcgggcg ccgtggcggg
       61 cggagccgaa ggtgccgtgc aagcagccaa agcgccgttc accaacgaac gccgcatcgt
      121 tcgcacttgg cgcaccgaaa ccaccaccga tggccgcacg atccaagtgc ccgtcgaaac
      181 cgaagtcgac gaacacggcc gccccatctc gtccaccgcc accaaataac cccacgcagg
      241 tggagcccga tgtcccatcg ggcttggttc gaaaagcgcc gcgtccaaac aagcccgatg
      301 gaatctcggg ctcgtgagat gcccggcaag agaagtgaat aactggagtg gcgcaggagc
      361 gccggtaggg accgatctcc gagcggtccg cagcccgctc actcgcacgc actcaaacaa
      421 aaaccccggc gtttccgccg gggttcgaga ctcactcgcg acctcttccg caatcgatcg
      481 ccggagtccg tgcagtcttg tagttcgtgc agtctgtagt tcctgtagtg gaatcttcgt
      541 tacgcgcgcc gctcaccagc cgacactcgc gcgcttcgtc atcttcgcga tcgagcgctc
      601 ttcttcccag accgtcagcc cgctcttcac atcgacgagc gacatctgga acgaatacgt
      661 gttctgcgtc tggctgcccg cgcgatcggt ctgctgaatc agctttccgt aaagcgtgta
      721 gtgcggagcg accagcttct gcttcttgcc cgtcgtcatc tcttcaagct ccgccgcttc
      781 cgatgcgacc accgcgcgct cgccgaggcc catcgtattc gtcacggcga tcttgccggt
      841 ctgcgtgagc gcgacgcgga tttttttgat caacaggtcc gtgtcgatcg acagcgtcgt
      901 gttgttgatc acgcggtcga ccgcgaggat cgccggctgc gccggcgcgc gttcgagcgc
      961 gctcgacgaa agcagcgact gcacgagctg gtcggccgca ttggcccagt cctgcgagtt
     1021 gatgctcgac gtgttgagcg cttcgggacc cttcgcatcg atcgtgcgcg tttccggcgt
     1081 ggtttcacaa ccggcgaaaa acagcgccgc aaccgcgagc gcggcggaca ccggtagttt
     1141 gttgaagtgg atctgattca tgagaggaag gaggacttat tcttttagct tcaggacaaa
     1201 atcgaccgcg cgcggcgtga ccgcgaccgt cgagatcgtg gtcgtctcgc gcggctgaac
     1261 gcgaatctgt ttccacgttt cgttcggcga gtccaccgcc atgccgttgg cgtcgaccca
     1321 ttcgaacttg tagctcaacg cgcgcgtcga gttcttcagg ttttcgatcg tcgcttggat
     1381 cttgagcagg ttgcccgaga ccgtggcttc gttgatggag accacgtgaa aggtcgcggc
     1441 gagcgtgcgg tcggtgacca cacgcttgtc ggcgacgtag ctcggcgacg cctgtgcttg
     1501 ggcacgctcg acggtgttca cggtcgtgcc acaacctgcg agcagcagcg tggcggtgat
     1561 tgcgagaaga ctggatttca tttgatggcg aattgcgaaa ccagcaacgg tgcggagctg
     1621 gacgcgctct tcacatatac gacgttcacg gcgcccggga cgagcgaaac cgtttgcgtc
     1681 gtcgtgcccg ccgtcagcgt gagctggcga tccgccggcg tcaggatgcg cgcatactga
     1741 aactccttcg gcaaactcgt c
//
LOCUS       DASPLT010000089         1197 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_42201, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000089 DASPLT010000000
VERSION     DASPLT010000089.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1197)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1197)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1197
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_42201"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..461)
                     /locus_tag="VEA63_00960"
     CDS             complement(<1..461)
                     /locus_tag="VEA63_00960"
                     /inference="COORDINATES: protein motif:HMM:NF012576.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HYD82576.1"
                     /translation="MAAPITQRDVARACGVHPSTICLALKNSPSIPLATRLKVQAAAE
                     ELGYRPNVAARNLALLRGDKSGAGSLPIAWLNQEAERNHWRTDPEARPAFDRARARAA
                     ELGYHLEEIWTREPGMTVGRLVQIVRARGIQGVLFPVHRAFDYSLVTPAWS"
     gene            567..>1197
                     /locus_tag="VEA63_00965"
     CDS             567..>1197
                     /locus_tag="VEA63_00965"
                     /inference="COORDINATES: protein motif:HMM:NF014376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family ligand binding domain-containing
                     protein"
                     /protein_id="HYD82577.1"
                     /translation="MRHAIDPTIKTRLLLRPVKSPVGTIRLGGINQGGFGIGRPPDPR
                     RTLSCFGFTYIMNGAGEYSDARVRNAPVGKGDLVIFFPGRPHQCSTRPGEFWDELWFE
                     CEGPVFDLMRSTGVLSPRQPLHHTQNSDFWFRRFFQIIPPLHLREKTPPQVIVTRFVA
                     VLNELLAGSEAPPESPAPREDDWLNRACELLTTHEGVAGLEPAQVARKLG"
BASE COUNT          220 a          396 c          347 g          234 t
ORIGIN      
        1 tcgctccacg ccggcgtcac cagcgagtag tcgaacgctc gatgcaccgg aaacaaaacg
       61 ccctggattc ctcgcgctcg cacgatctgg acgagccgtc ccaccgtcat tccgggttcg
      121 cgcgtccaaa tttcttccaa gtgatacccc agctccgccg cgcgcgcacg cgcccggtca
      181 aacgccggac gcgcctcggg atcggtgcgc caatgattgc gctctgcttc ctgattcagc
      241 catgcgattg gcagactgcc cgcgccgctt ttgtcgccac gcaacaaagc gagatttcgt
      301 gcggcgacgt tcggccgata cccaagctcc tcggcggccg cctgcacctt caagcgcgta
      361 gcgagcggga tggagggcga gtttttcaac gcgagacaga tcgtgctggg gtgaacaccg
      421 caggcacggg caacgtcacg ctgagtaatg ggggcagcca cagacatgaa cttgttgggg
      481 agtaaaacgt cgtcactcta gccttacttt gctagttgcg gaatggctca actggtagaa
      541 aacatgtgtt tcttggaagg tctcccatgc gccacgccat cgatcccaca atcaagaccc
      601 gcctgctgct ccgaccggtg aaatcgccgg ttggcacgat ccgtctcggc ggcatcaacc
      661 aaggcggttt cggcattggc cgcccacctg atccgcggcg cacgttgagc tgcttcggtt
      721 tcacctatat catgaacggc gcgggcgaat attccgacgc ccgtgtgcgc aacgctccgg
      781 tcggcaaggg cgacctcgtg attttttttc ccggccggcc gcatcagtgc agcacacgac
      841 cgggcgaatt ctgggacgag ctctggttcg aatgcgaagg cccggtgttc gatctcatgc
      901 gttccaccgg tgttctctcg ccgcgccagc cgctgcatca cactcagaac agcgatttct
      961 ggttccgccg tttcttccag atcattccgc ctctgcacct ccgtgaaaag acgccgccac
     1021 aggtcatcgt cacgcgcttc gtcgccgtgc tgaatgaact cctcgccggt agcgaggcgc
     1081 cgccggaatc acccgctccg cgggaagacg attggctcaa tcgcgcgtgc gagctgctga
     1141 cgactcacga gggcgtcgcc ggactcgaac cggcgcaggt cgcgcgcaaa cttggtt
//
LOCUS       DASPLT010000090         1341 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_267361, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000090 DASPLT010000000
VERSION     DASPLT010000090.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1341)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1341)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1341
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_267361"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1037
                     /locus_tag="VEA63_00970"
     CDS             <1..1037
                     /locus_tag="VEA63_00970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD82578.1"
                     /translation="YVAKAAGLKLESYSQQVETAVSLQRVSARTAKVKHLDLGRVRIK
                     GAMAFIISNDDLVNVSAVSAAWGDRLDGVLGMATVADVLLEIDYPRKQVSVALPAAHF
                     YPVERAVSYRDRGGVPEVVLEIGGRMFPMDLDTGALATAITVDDATGLELVHPWIKQD
                     GGDIGIGTDNVRRERSQLAGDARLGPITWRNPPLSKSLGARIGLDALSSWRLVFDQRE
                     QKIYFLDAEPGLAWEIQAPPDPQRMLGFFGEIINGAAVRLGEVDGGKAFDRAGLRAGD
                     ILETVNGVPAFLWLVSDWDRAEPEQLRIRAVRAGIEFEVTAVRGLDEPIREVEPQEDA
                     ASKYLSSSPD"
     gene            1056..>1341
                     /locus_tag="VEA63_00975"
     CDS             1056..>1341
                     /locus_tag="VEA63_00975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HYD82579.1"
                     /translation="MNSSPLDFRNTNALWGSVFVETLVRCGVRQAVISPGSRSTPLTM
                     ALARHPAIDAVPVLDERSAAFFALGVAKRTSRPVVLLCTSGTAGANYFPAV"
BASE COUNT          246 a          354 c          463 g          278 t
ORIGIN      
        1 agtatgtggc gaaagcggcc ggactgaaac tggaaagcta tagccaacag gtcgaaaccg
       61 cggtatcgct acagcgggtt tccgcacgca cggcgaaagt gaagcacctc gatctggggc
      121 gtgtgaggat caaaggcgcg atggctttca tcatctcgaa tgacgatctg gtcaatgtgt
      181 ctgccgtgtc tgccgcgtgg ggcgatcgcc tggatggtgt cttggggatg gctaccgtgg
      241 cggacgtttt gcttgagatc gattacccac ggaagcaggt gagcgtcgcg cttcccgccg
      301 ctcactttta tcctgtcgag cgcgcggtga gctatcggga tcgtggtggc gtcccggagg
      361 tcgtgctcga gatcggcgga aggatgttcc cgatggacct cgatacgggt gcgctggcga
      421 ccgccatcac ggtcgatgat gcgacagggc tcgaactcgt gcatccctgg atcaagcaag
      481 atggcgggga tattgggatc ggaacagata acgtccgtcg cgagcggtca caacttgccg
      541 gcgatgcgcg gctggggccg atcacctggc gtaatccgcc gctgagtaag tcgctcggtg
      601 cgaggattgg attggatgcg ctgagctcgt ggcgcctcgt cttcgatcaa cgcgaacaga
      661 aaatctattt cctcgacgcc gaacccggcc ttgcgtggga aatccaagct cctcccgatc
      721 cacagcgcat gctcgggttc ttcggcgaga tcatcaacgg agcggcagtt cgtttggggg
      781 aagttgacgg ggggaaggcg ttcgaccgcg ccggactccg tgccggagat attttagaaa
      841 cggtgaatgg agtgcctgcg ttcctatggc tcgtgtcgga ttgggatcgt gcggagccgg
      901 agcagttaag gattcgcgcc gtccgagccg gaattgaatt tgaagtaact gccgttcgtg
      961 gactggatga gccaatccgc gaagtcgaac cccaggagga cgcggccagt aaataccttt
     1021 cgtcgagccc cgattaacct caacgagagt atctgatgaa ttcctcaccg cttgatttcc
     1081 gaaacacgaa cgcgctctgg ggcagcgttt ttgtggagac gctggtgcgg tgtggcgtgc
     1141 ggcaggcggt gatttcgccg ggatcgcgct cgacgccgct gacgatggcg ctggcgcggc
     1201 atccggccat cgacgcggtg ccggtgctcg atgagcgatc ggcggcgttt ttcgcgcttg
     1261 gggtcgctaa gcgcacgagt cggccggtgg tgctgctgtg cacgagtggg acggccgggg
     1321 cgaactattt tccggcggtg a
//
LOCUS       DASPLT010000091         1275 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_22395, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000091 DASPLT010000000
VERSION     DASPLT010000091.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1275)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1275)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1275
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_22395"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1222
                     /locus_tag="VEA63_00980"
     CDS             <1..1222
                     /locus_tag="VEA63_00980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD82580.1"
                     /translation="WPHRQVTPEVLIRRLPRATEAFRWWTDEDRAGRTYHRLSGLDAE
                     ELKFVVDTAVPFAELKWSQEISGYSLDQLERAYTMIAYREDRAAKQIGMWPEKSYTLP
                     TILAAGGICVDQTYFATAIGKARGVPTLFFEGAGRDGRHAWFGFLDGKRKWQMDVGRY
                     ASQRLVTGFARDPQTWGTLSDHALAFLAERFRTTASYRRSQIHADFAADFLAYGDAAA
                     AAKAARRSVDAERRNQAGWDALFAAEEAFGKPAKEREATLHEAIVALQRYPDMEVSYS
                     KRLSDSLRARGETSAATQEQQRIAGKYQGSRADLSVRQAKDLLLRSIDTQPVREQVRA
                     YNNLVDKYARSAGVGFFDQIVVGFVEHLVQMKEPKAALEAMNRAGRVLKVKSGSQLDH
                     DFQRLLKALKEMRP"
BASE COUNT          242 a          345 c          466 g          222 t
ORIGIN      
        1 ctggccgcat cgccaggtga cgccggaggt gctgatacgc cggctgccgc gagcgacgga
       61 ggcgtttcgc tggtggaccg atgaggaccg cgcgggtcgc acctatcacc gactctccgg
      121 cctcgatgcg gaagagttga agttcgtcgt cgatacggcg gtgccgttcg cggaactcaa
      181 gtggtcgcaa gagatctcgg gctactcgct cgatcagctc gagcgcgcct acacgatgat
      241 tgcgtatcgc gaagatcgcg cggcgaaaca aatcggcatg tggccggaga aaagctacac
      301 gctgccgaca atcctcgcgg cgggcgggat ttgcgtggac cagacctatt tcgcgacggc
      361 gatcggaaaa gcgcgcggcg tgccaacgct gtttttcgaa ggcgccggtc gtgacggacg
      421 tcacgcgtgg ttcggttttc tcgacggaaa gcgtaaatgg cagatggatg tcggccgtta
      481 cgcgtcgcag cggttggtga cggggtttgc gcgcgatccg cagacctggg gcacgctctc
      541 ggatcacgcg ctggcgtttc tggcggagcg gtttcgcacc acggcgtcgt atcgtcggtc
      601 gcaaattcac gcggatttcg cggcggattt tctcgcgtat ggggacgcgg cggcggcggc
      661 gaaggcggcg agacgatcgg tcgacgcgga gcggcggaat caagccggct gggacgcttt
      721 gttcgccgcg gaggaggcgt tcggcaagcc tgcgaaggag cgtgaggcga cgctccacga
      781 ggcgatcgtc gcgctgcagc gttatcccga tatggaagtg agctattcga agcgtttgag
      841 cgacagcctg cgtgccagag gcgagacgag cgcagcgacg caggaacagc agcggatcgc
      901 gggcaagtat cagggcagcc gcgcggactt gagcgtgcgc caggcgaaag acctgctgtt
      961 gcgcagcatc gacacgcagc cggtccgcga acaggtgcga gcttacaata acctggtgga
     1021 taaatatgcg cgcagtgcgg gcgtcggctt cttcgaccag atcgtcgttg gattcgtcga
     1081 acacttggtg cagatgaagg agccgaaggc ggcgctggag gcgatgaacc gcgcggggcg
     1141 cgtgttgaag gtcaaatcgg gcagccagct cgatcacgat tttcagcggc tgctgaaagc
     1201 gctgaaggaa atgcggccgt agtcgctgct tgggagacgt tgggaatcac cgttgaacag
     1261 ggagcgcgag cggat
//
LOCUS       DASPLT010000092         1054 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_83102, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000092 DASPLT010000000
VERSION     DASPLT010000092.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1054)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1054)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1054
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_83102"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(40..969)
                     /locus_tag="VEA63_00985"
     CDS             complement(40..969)
                     /locus_tag="VEA63_00985"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HYD82581.1"
                     /translation="MNAPTELAIDVAGITKRFGSKTVVDAIDLRVRRGEIFGFLGPNG
                     SGKTTFIRMLCGLLTPDAGAGTCLGYDVRTQQAEIKRHVGYMTQRFSYYEDLSIRENL
                     DFVARVYDVPERRDAVEQSLERLGLQNRSAQLAGQLSGGWKQRLALAACLIHSPQLLL
                     LDEPTAGVDPKARRDFWDEIHRLAAGGLTVLITTHYMDEAERCHRLAYLAYGKLLAQG
                     TVAEVVTAARLVTWTVSGPELHRVAEALRGREGVEQVVAFGNTLHVSGRDAGKLEVAI
                     AAAREPQHEWSRAHAGLEDVFISLMDQAEDNFR"
BASE COUNT          185 a          423 c          259 g          187 t
ORIGIN      
        1 ggctcgctcc gtaaggagcg ggacgtttgc cgcccgcctt catcggaaat tatcctccgc
       61 ctgatccatc aagctgatga acacatcctc gagccccgcg tgcgcccgac tccactcatg
      121 ctgcggctcg cgcgccgccg cgattgccac ctccagcttc cccgcatcgc gtccgctcac
      181 gtgcagcgtg ttcccaaacg ccaccacctg ctccacccct tcgcggccgc gcaacgcttc
      241 cgcgacccgg tgcagctccg gcccgctcac cgtccacgtc accagccgcg ccgcggtcac
      301 cacctccgcc accgtgcctt gcgcgagcag tttcccatac gccagataag ccagccggtg
      361 acatcgctcc gcctcgtcca tgtaatgcgt cgtgatcaac accgtcagcc cgcccgccgc
      421 gagccggtga atctcatccc aaaaatcccg ccgcgccttc gggtccacgc ccgccgtcgg
      481 ctcgtccagc agcaacaact gcggcgaatg aatcaaacac gccgccagcg cgagccgctg
      541 cttccatccg cccgacaact gccctgcgag ttgggcactg cggttctgca atcccagtcg
      601 ctccaaactc tgctccaccg cgtcgcgacg ctccggcacg tcgtaaaccc gcgccacgaa
      661 atccaggttt tcccgaatgc tcaggtcctc gtaatagctg aaacgctgcg tcatgtaacc
      721 cacgtggcgc ttgatctccg cctgctgcgt ccggacatcg tagccgagac acgtgcccgc
      781 gcccgcatcc ggcgtcagca gtccgcagag catccggata aacgtcgtct ttcccgaacc
      841 gttcggcccg agaaatccaa aaatctcccc gcgtcgcacc cgtaaatcga tcgcatccac
      901 cacggttttc gagccaaacc gcttcgtgat ccccgccaca tcgatcgcaa gttccgtcgg
      961 cgcattcatg ttcgtagtag ggcgggcttc agcccgcctc atcgcgtcac atccactggc
     1021 tgccccggat gcagttcatc cgccgcgtcg acgc
//
LOCUS       DASPLT010000093         1128 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_144693, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000093 DASPLT010000000
VERSION     DASPLT010000093.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1128)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1128)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1128
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_144693"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..800)
                     /locus_tag="VEA63_00990"
     CDS             complement(<1..800)
                     /locus_tag="VEA63_00990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HYD82582.1"
                     /translation="MSRFADTIAALATPTGTSAIAVVRLSGPDTVRLTREIFGDVPPP
                     RHLRRADYRDRGGRLLDDVLFVFFQAPQSYTGEDSLEISCHGNPFIAQQIVEDLLARG
                     CRAAEAGEFTQRAFLNGQMDLSQAEAVVDVIHARSERALAAANRQLRGALGRRVGRTV
                     EALLGVLARIEAYIDFPDEDLPAEDRELVDASIVELINDTSKLLSTNRYGDILRSGIK
                     TVIVGKPNVGKSSLLNRLLGSERALVSPEPGTTRDFLEEPIMVGPHYLR"
     gene            complement(918..>1128)
                     /locus_tag="VEA63_00995"
     CDS             complement(918..>1128)
                     /locus_tag="VEA63_00995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD82583.1"
                     /translation="GAVFQRGLQWQTFVMEDGRAVLRDVEVGRTNGRETEIVSGVGEG
                     ERVVVYPGDKIADGVRVRRVELESH"
BASE COUNT          230 a          412 c          291 g          195 t
ORIGIN      
        1 cgcaggtaat gcgggcccac catgatcggc tcttccaaaa agtcgcgggt cgtccccggc
       61 tcgggactca ccaacgcccg ttcgctccct aataaacggt tcagcaaact gctctttccc
      121 acattcggtt tgcccacgat caccgtcttt attccgctgc gcaaaatatc accgtagcga
      181 ttggtagaca acaatttcga cgtatcgttt atcagttcga caatgcttgc atcaaccaac
      241 tcgcgatcct ccgcaggaag atcttcgtcc ggaaaatcga tgtaagcttc gatccgcgcc
      301 agaacgccca ataacgcctc gacggtgcgc ccgacacgcc gtcccagagc cccgcgcaac
      361 tgtcggttag ccgcagccag cgcgcgttcg ctccgcgcat ggatcacatc cacgacggct
      421 tccgcctggc tcaaatccat ttgtccgttg agaaaagccc gttgcgtgaa ttcgccggcc
      481 tccgccgcgc gacagccgcg cgccagcaaa tcctccacga tttgttgtgc gatgaacgga
      541 ttcccgtggc aggaaatctc cagcgagtct tcgcccgtgt aggattgcgg cgcctggaaa
      601 aacacaaaca gcacgtcgtc cagcaaacgc ccgccgcgat cgcgatagtc ggcgcgccgc
      661 aggtgacggg gcgggggaac atcgccaaaa atctcccgcg tcaaccgcac tgtatccggt
      721 ccgctgagac gcaccacggc gatcgccgac gtcccggtgg gcgtcgcgag cgcagcgatg
      781 gtatcggcaa aacgtgacat gaagcgcccc accatagcgg cgcctcgtgg aacgcaaagc
      841 gatttgcgag ccgccacgcc aacgcgcacg ccgatcaccc gtgcaaccgc cgcgcacgtc
      901 cccgatccac accgcgtcta atgcgattcg agttcgaccc gtcggacgcg aaccccgtcc
      961 gcgatcttgt cgcccgggta aacgaccacg cgttcaccct cgccgacgcc actcacgatc
     1021 tccgtctccc gcccgttcgt gcggccgacc tccacgtcgc gcagcaccgc ccggccatct
     1081 tccatcacaa atgtttgcca ctgcagcccg cgctgaaaca ccgcgccc
//
LOCUS       DASPLT010000094         5212 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_247045, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000094 DASPLT010000000
VERSION     DASPLT010000094.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 5212)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5212)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5212
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_247045"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(8..748)
                     /gene="hisA"
                     /locus_tag="VEA63_01000"
     CDS             complement(8..748)
                     /gene="hisA"
                     /locus_tag="VEA63_01000"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007363142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HYD82584.1"
                     /translation="MTIYPAIDIKGGRCVRLTQGRADQETVYAENPADVAAQFRASGS
                     GWVHVVDLDGAFTGEPQNLAAVQAIAAVGMKVQLGGGMRTRAAVERALGFGVSRVVIG
                     TRAAESEAFVGELVQAFGEKIAVGIDAKNGQVAVKGWVATAELSAVALARRMDALGVA
                     TLIHTDIGTDGMLTGPNYAAQEAMLNAGKFRVIASGGVSRREDVMELTKLAQRHANLD
                     GVIVGKALYEQRVELGDLLAIARGEAAE"
     gene            929..1993
                     /locus_tag="VEA63_01005"
     CDS             929..1993
                     /locus_tag="VEA63_01005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007363203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HYD82585.1"
                     /translation="MAKYQVPAFLSELLHARSPSGYETEAQAVFDRHVKPAADAYAND
                     ALGNRLATLNPKGDPVLMLAGHMDELGLIITYINKDGFIYFDTVGGHDLSVISGRRVI
                     IQTENGPVKGVTGKRAIHLMDDEDRKKVPKKHEIWIDIGARSKKEALARVAIGDVATY
                     DHELELINGSIGTARAFDNKVGAYIVGETLIRLSREKKLAAKVVAVATSQEEIGTRGA
                     TTATYTVNPHIAVAIDVGHATDHPDCDNRKYGETKLGGGPILCRGANINPKIYQRLVA
                     AAKKADIPYQLEADPRPTGTDARAIQMGRGGVATGLVSIPLRYMHTPSEVVDLEDVER
                     CVKLLVEFAKALTKGDYAHW"
     gene            2107..3663
                     /locus_tag="VEA63_01010"
     CDS             2107..3663
                     /locus_tag="VEA63_01010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015333 - peptide:proton symporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide:H+ symporter"
                     /protein_id="HYD82586.1"
                     /translation="MSTPPANPTALADHVPRIPRQIPYIIGNEGCERFSFYGMRNILT
                     VFLVSSLLVHLPEAERGAAAKDVFHTFVIGVYFFPLLGGWLADRFFGKYFTIFWLSLV
                     YCVGHLCLALFESSRPGFYTGLFLIALGSGGIKPLVASFAGDQFDQSNKHLAKVVFDA
                     FYWIINFGSFFASLLMPIFLRSFGPSVAFGIPGALMAIATFILWLGRKRYVMMPPAPP
                     NPHSFLRVAATALASGPRGRTLASIGAVAALASLALIPAAGFVVAVCTALVVALAFGG
                     IGVWLQLDAARNRHPDEAVDGVRSVLQVLVLFFLVTPFWSLFDQKASTWVLQADAMTK
                     PHWFQSSQMQALNPLLVMLLIPFNNLVFYPALKRLGWEPTPLRRMTYGIAFAGLSWIV
                     VGAMQLALDGGHAFSITWQVLPYALLTLGEVLVSATGLEFAYSQAPHAMKGVLMSFWT
                     LSVTIGNLWVLIVNGAVKNETVTNLIASTGFGITAFQMFFFAVFAFAAALAFGLYARR
                     YHMADHYRKT"
     gene            complement(3876..5039)
                     /locus_tag="VEA63_01015"
     CDS             complement(3876..5039)
                     /locus_tag="VEA63_01015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endo-1,4-beta-xylanase"
                     /protein_id="HYD82587.1"
                     /translation="MKLRCLFGAAVLLVTFNAGARLVAAPGGELGSLKEAAEGLFHVG
                     AAINSRQITEQDERGVKVVEAHFDSITPENVMKWVVIHPEPERYDFELADKFVEFGEK
                     RGLFIIGHTLMWHSQTPEWVFKDEDGNDVAKDVLLKRLREHIHTVVGRYRGRVKGWDV
                     VNEAVKDEDGNIRLDRPWYRILGEEGIVEAFKAAHEADPDAELYYNDYSLANPVKRAG
                     ALRLVQLIRAKGGRIDGVGTQEHCSLDWPTVEAVDATIRDFAQAGFKVMVTELDVTVL
                     PRPREYYGAEISTVFESAPELDPYRAGLPADKEAELAKRYGELFAVFAKHRGALTRVT
                     LWGVSDRGSWLNNFPIRGRTDHALLFDRDYQPKPALEAAIRALKPEADPALKR"
BASE COUNT          917 a         1990 c         1317 g          988 t
ORIGIN      
        1 gcgtggacta ctccgccgct tcgccccgcg cgatcgccag tagatcgccg agctccacgc
       61 gctgctcgta aagcgctttg cccacgatca cgccgtccag attggcgtga cgctgcgcta
      121 gctttgtcaa ttccatgaca tcctcgcgcc ggctcacgcc gccactcgcg atcacgcgaa
      181 attttcccgc gttcaacatc gcctcctgcg ccgcgtaatt cggacctgtc aacatcccgt
      241 cagtcccgat gtccgtgtga atcagggtcg cgacgcccaa cgcatccatc cgccgcgcca
      301 gcgccaccgc gctcaactcc gccgtcgcga cccagccctt caccgcgacc tgcccgttct
      361 tcgcgtcgat gcccaccgcg atcttttcgc cgaacgcctg cacgagctcg cccacgaacg
      421 cttcgctctc cgccgcgcgc gtgccgatca cgacgcggct cacgccgaag cccagcgccc
      481 gctcgactgc cgctcgcgtg cgcatgccgc cgccgagctg cactttcatc cccacggccg
      541 cgatcgcctg caccgccgcc agattctgcg gctcacccgt gaacgcgcca tcgagatcga
      601 ccacgtgcac ccaaccgctt ccgcttgcgc gaaactgcgc cgcgacgtcc gccggattct
      661 cggcgtaaac ggtttcctgg tcggcgcgtc cctgcgtcag ccgcacacag cggccgccct
      721 tgatgtcgat ggcgggataa atggtcatga ggaaccgcga ggctcgcgca aacttcgccc
      781 agcgcaaaag cttttttccg ccgctcgcag actttgccgg tagggacggc tctccgagcc
      841 gtccgcgccg accaccgacg cgcgattcaa ccgatatcat cgcgaatttc atctttgcgc
      901 cgccggcact ttgcctgagt ttagcgtcat ggccaaatac caggtccccg ctttcctttc
      961 cgaattgctg cacgcgcgct cgccttccgg ttacgaaacc gaggcgcaag ccgtcttcga
     1021 ccgccacgtc aaacccgccg ccgacgccta cgccaacgac gcgctcggca atcgcctcgc
     1081 cacgctcaat cccaaaggcg accccgtgct catgctcgcc ggccacatgg acgagctcgg
     1141 cctcatcatc acctacatca acaaagacgg ctttatctat ttcgacaccg tcggcgggca
     1201 cgacctcagc gtcatctccg gtcgccgcgt catcattcaa acggaaaacg gtcccgtcaa
     1261 aggcgtcacc ggcaagcgcg cgattcatct catggacgac gaggatcgca agaaggtgcc
     1321 caagaagcac gagatctgga tcgacatcgg cgcgcggtct aaaaaagaag cgctcgcccg
     1381 cgtcgcgatc ggcgacgtcg ccacctacga tcatgagctc gagttgatca acggcagcat
     1441 cggcaccgcg cgcgccttcg acaacaaggt cggcgcctac atcgtcggcg aaacgctcat
     1501 ccgcctttcg cgcgaaaaga aactcgccgc caaggtcgtc gccgtcgcca cctctcagga
     1561 agagatcggc acccgcggcg ccaccaccgc gacttacacg gtcaaccctc acatcgccgt
     1621 ggcgatcgac gtaggccacg cgaccgatca tcccgattgc gacaatcgca aatacggcga
     1681 aacgaaactc ggcggcggcc cgatcctctg ccgcggcgcc aacatcaatc cgaaaatcta
     1741 ccagcgcctc gtcgcggccg cgaaaaaagc ggacatccct tatcaactcg aagccgaccc
     1801 gcgtccgacc ggcaccgatg cccgtgcgat ccagatgggc cgcggcggcg tcgcgaccgg
     1861 cctcgtcagc atccccctcc gctacatgca cacgccgagc gaagtcgtcg atctcgagga
     1921 cgtggagcgt tgcgtcaaac tcctcgtcga attcgcaaag gcgctcacca agggcgacta
     1981 cgcccactgg taatccaccc tccagcgatc tcaccgtagg gcgggtttca acccgccctt
     2041 tttttgcgcc cattcctctc ccttgcgctt cgccctcgcg cccgcagcgt tgcacgcacg
     2101 ccgctcatga gcacgcctcc cgcgaacccc accgcgctcg ccgaccacgt gccgcgcatt
     2161 cctcgccaga tcccctacat catcggcaac gagggctgcg agcgcttcag cttctatggc
     2221 atgcgcaaca tcctcacggt gttcctcgtg tcgtcgctgc tcgtgcactt gcccgaagcg
     2281 gaacgcggcg ccgccgcgaa ggacgtcttt cacacgttcg tcatcggcgt ttattttttc
     2341 ccgctgctcg gcggctggct cgccgatcgc ttcttcggaa aatatttcac gatcttctgg
     2401 ctcagtctcg tctactgcgt cggccatctt tgcctcgcgc tcttcgaatc gagccgcccc
     2461 ggtttctaca ccggactctt cctcatcgcg ctcggctccg gcggcatcaa gccgctcgtc
     2521 gcatccttcg ccggcgacca gttcgaccaa tccaacaagc acctcgcgaa agtcgttttc
     2581 gacgcctttt actggatcat caacttcggc tcgttcttcg cctcgctgct gatgccgatc
     2641 ttccttcgca gcttcggccc gagcgtcgcc ttcggcatcc ccggcgcgct catggccatc
     2701 gcgacgttca tcctctggct cggccgcaaa cgctacgtca tgatgccgcc cgcgccgccc
     2761 aacccgcact cgttccttcg cgtcgccgcc accgccctcg cgtcgggtcc gcgcggtcgc
     2821 accctcgcaa gcatcggcgc agtcgccgcg ctcgcctcgc tcgccctcat ccccgccgcc
     2881 ggtttcgtcg tcgccgtctg caccgccctc gttgtcgcgc tcgctttcgg cggcatcggc
     2941 gtgtggttgc aactcgacgc cgcacgcaac cgccaccccg acgaagccgt cgacggcgtg
     3001 cgctccgtgc tgcaggtgct cgtgctgttt ttcctcgtga cgcccttctg gtcgctcttc
     3061 gaccaaaagg cgtcgacgtg ggtcctgcag gccgacgcga tgacaaagcc gcactggttt
     3121 caatcctcgc agatgcaggc gctcaatccg ttgctcgtca tgctgctcat cccgttcaac
     3181 aacctcgtat tctatcccgc gctcaagcgc ctcggctggg agccgactcc cctgcgccgc
     3241 atgacctacg gcatcgcctt cgccgggttg tcgtggatcg tcgtcggcgc catgcagctc
     3301 gcactcgacg gcggtcacgc gttttccatc acgtggcagg tgctgcccta cgcactgctc
     3361 acactcggcg aagtgctcgt ctccgccacc ggcctcgagt tcgcctacag ccaggcgccg
     3421 cacgcgatga agggcgtgct gatgagtttc tggacgctct ccgtcaccat cggaaatctc
     3481 tgggtcctga tcgtcaacgg cgccgtgaaa aacgaaaccg tcacaaacct catcgcttca
     3541 accggcttcg gcatcaccgc gtttcaaatg tttttcttcg ccgtcttcgc cttcgccgcc
     3601 gcgctcgcct tcggtctcta cgcccgccgc taccacatgg ccgaccacta ccgaaaaacg
     3661 taagcgctca ctccgaaaag gaaaccacgg atgaacacat atcccgcacc agaaatgaaa
     3721 ccacgaatgg acacgaattc acacgaatag aaatccaagg catcgaacgc ccacgccctt
     3781 cattcgtgtg aattcgtgtc cattcgtggt taaaacagtt tgtcagtcca atccgtgttc
     3841 atccgtgctc atccgtggtt aaaaattaac cagccttacc gcttcaacgc cggatccgct
     3901 tccggcttca acgcccggat cgccgcctcc agcgccggct tgggttgata gtctcgatcg
     3961 aacaacaacg cgtgatcggt ccgtccgcga atcgggaaat tgttcagcca cgaaccgcga
     4021 tccgatacac cccacagggt cacgcgcgtc agcgcgccgc gatgtttcgc gaacaccgcg
     4081 aacaactcgc catagcgctt cgccagctcc gcctccttgt ccgccggtaa acccgcgcga
     4141 tacggatcca gctccggcgc gctctcgaac accgtcgaga tctccgcgcc ataatactcg
     4201 cgcggccgag gcagcaccgt cacgtcgagc tccgtcacca tcactttaaa acccgcctgc
     4261 gcgaaatcgc ggatcgtcgc gtccaccgcc tccaccgtcg gccagtcgag cgaacaatgc
     4321 tcctgcgtcc ccacgccgtc gatccgcccg cccttcgcgc ggatcaactg caccagccgc
     4381 agcgcgcccg cgcgcttcac cggattcgcg agcgaatagt cgttgtagta aagctccgcg
     4441 tccggatccg cttcgtgcgc ggccttgaac gcttcgacga tcccttcctc gccgagaatt
     4501 cgataccacg gccggtccag ccgaatgttg ccgtcctcgt ccttcaccgc ctcgttcacc
     4561 acgtcccaac ctttcacgcg tcccctgtag cggccgacca ccgtgtggat gtgttcgcgt
     4621 agtcgtttta acaatacgtc cttcgccacg tcgttcccat cctcgtcttt gaagacccac
     4681 tcgggcgtct ggctgtgcca catgagcgtg tggccaatga tgaacagccc gcgcttctcc
     4741 ccaaactcca cgaacttgtc cgccagctcg aaatcgtagc gctccggctc gggatgaatc
     4801 accacccact tcatcacatt ctccggcgta attgagtcga agtgcgcctc cacgaccttc
     4861 acgccgcgtt cgtcctgctc cgtgatctgc cgcgagttga tcgccgcgcc gacatgaaac
     4921 aacccttccg cggcctcctt caacgaacca agttcaccgc ccggcgccgc aacgagccgc
     4981 gcaccggcgt tgaacgtgac caacaatact gccgcaccaa agagacaacg aagtttcatc
     5041 gggagcgaac ttgcgcagct cgctctccaa caccagccgc ttctgcttcg cgcagcgctc
     5101 ttgtccgccc cgccccatcc ggcctcccac ccacgccgcg gcgctcccct ttccccaccg
     5161 ccgcgtccgc gccccgcccg gttgcgaccg gctccgcgcc gcttaatttg ac
//
LOCUS       DASPLT010000095         1138 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_165396, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000095 DASPLT010000000
VERSION     DASPLT010000095.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1138)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1138)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1138
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_165396"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(135..>1138)
                     /locus_tag="VEA63_01020"
     CDS             complement(135..>1138)
                     /locus_tag="VEA63_01020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HYD82588.1"
                     /translation="APSGAEREGGKQRAVKRPTIYDLARIAEVSPGTVSRVLNNRDKV
                     KAETRERVLRAANALNLKPQVSVRFRQVAILSEPNFPDRIEGYAATLTAHLSFAFSRR
                     NIGVVLPSNPFEQLPTLFLDGLIAVTFDKQLEALLADLENRMHVVYMDKFQLQPNEYV
                     VRSDHFNSGSLAAQHFIARGKRRFGFLGGNNNAPFVERLKGYRQAIADAGHTIDERLL
                     VLAGPETNHLSAITRIVRAGADCIYVPGTTFQAMECLHILSYVMGVKVPQDISIIGGE
                     NEGISAIQNPPLTTIEDPLREMAEQAADMLDKLTSGQRVAQRHLTLPVRLIERDSVS"
BASE COUNT          190 a          285 c          438 g          225 t
ORIGIN      
        1 caagggacgc ccctacgccc cgtcttctca tggtcgcgat cgcggctttc ggacgcgttt
       61 gctgaaggaa gcgcggcgat gcattgctgt tccgctgccg agtgtcgggc gaattcatcg
      121 cggtgaggtg tcggctacga aacggaatcg cgctcgatca ggcgcacggg aagcgtgagg
      181 tggcgctggg cgacgcgttg accggaggtg agcttgtcga gcatgtcggc agcttgctcg
      241 gccatttcgc gcaggggatc ttcgatggtg gtgagcggcg gattttggat cgcggagatg
      301 ccttcgtttt cgccgccgat gatcgagatg tcctggggga cttttacgcc catgacgtag
      361 ctcaaaatgt gcaggcattc catcgcttga aaggtggtgc cgggcacgta aatgcagtcg
      421 gcgccggcgc gaacgatgcg cgtgatggcg gagaggtgat tggtttccgg gccggcgagg
      481 acgaggaggc gttcgtcgat ggtgtggccg gcatcggcga tggcttggcg gtagcctttg
      541 aggcgttcga cgaaaggagc gttgttgttg ccgccgagga aaccgaagcg gcgtttgccg
      601 cgggcgatga agtgttgggc ggcgagcgag ccggagttga agtggtcgga gcggacgacg
      661 tattcgttcg gctggagctg aaacttgtcc atgtagacga cgtgcatccg gttctcgaga
      721 tcggcgagga gcgcctcgag ctgcttgtcg aacgtgaccg cgatgaggcc gtcgaggaag
      781 agcgtcggaa gttgctcgaa cggattggaa ggaagcacga cgccgatgtt gcggcgggaa
      841 aacgcgaacg agagatgtgc ggtgagcgtc gcggcgtagc cctcgatgcg gtcgggaaaa
      901 ttcggctcgg agagaatcgc gacctggcga aagcgaacgg agacctgtgg cttgagattg
      961 agcgcgttgg cggcgcggag cacgcgttcg cgagtctccg ctttgacctt gtcgcggttg
     1021 ttcaacacgc ggctgaccgt gccgggcgag acctcggcga tgcgcgcgag gtcgtagatg
     1081 gtggggcgct tcacggcgcg ttgcttgccg ccttcgcgtt ctgcgccgct cggggcgg
//
LOCUS       DASPLT010000096         1880 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_22489, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000096 DASPLT010000000
VERSION     DASPLT010000096.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1880)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1880)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1880
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_22489"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1263
                     /locus_tag="VEA63_01025"
     CDS             <1..1263
                     /locus_tag="VEA63_01025"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HYD82589.1"
                     /translation="ALFDVNLPDGRSLELLAEKTVPTSVVTVVMTAEGGIDGALEATR
                     LGAADYLVKPFDVDDLGLRLARAWRDRQKKRGDQFQRDQRAAADESFFFGAGLAAVET
                     QLGKIIAADRRLQNGALPPVLIEGETGTGKTTLARWIHRRGPRADGPLIEVNCSALPE
                     NLAEAELFGHERGAFTDAKEARIGLLEAAEGGTLFLDELPSLSLGVQAKLLKAIEDRA
                     IRRVGGTRTVEIDARIVAATNVDLRALVADGKFREDLLHRLDLFRVRIPPLRERGDDV
                     IALAETLVARFAKKYGLAPRPIPAAGRERLRAHRWPGNVRELMHEIERSLVFDDDHLT
                     FAGLAAATRAQADNNSQLLPPDFHFPSGFSLEATIDAFVQRALDQSDGNVSAAARLLG
                     VPRDYVRYRIKTRKPAAAELSDVSPHAD"
     gene            1523..>1880
                     /locus_tag="VEA63_01030"
     CDS             1523..>1880
                     /locus_tag="VEA63_01030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD82590.1"
                     /translation="MTKNILISIALAGGISAGAINVSAQNAVDVLKTDAGVSVPAVTF
                     TPSERPAAPIEGNVVPIDHVIALPDFVDAVPVPITRPLPDFQIDTSHAFDGNVAVRPD
                     VSVNPLDPTQLNVVEIG"
BASE COUNT          341 a          731 c          492 g          316 t
ORIGIN      
        1 gctctcttcg acgtcaacct gcccgacggt cgcagtctcg agctcctcgc ggaaaaaacc
       61 gtgcccacca gcgttgtcac tgtcgtgatg accgccgagg gcggaatcga cggcgcgctc
      121 gaagcgaccc gcctcggcgc ggccgattac ctcgtaaaac cgttcgacgt cgacgatctc
      181 ggcctgcggc tcgcccgcgc ctggcgcgac cgccagaaga aacgcggcga ccagttccaa
      241 cgcgaccagc gcgcggcggc cgacgagtcg tttttctttg gcgccggcct cgccgccgtc
      301 gaaacccagc tcggcaaaat catcgccgcc gaccgccgcc tccaaaacgg cgcccttcca
      361 cccgtcctca tcgaaggcga aactggcacc ggcaaaacca ccctcgcccg ctggattcac
      421 cgccgcggtc cgcgcgccga cggcccgctc atcgaggtca actgctccgc gcttcccgaa
      481 aacctcgccg aagcggaact gttcggtcac gaacgcggcg cgttcaccga tgcgaaagaa
      541 gcacgcatcg gcctcctcga agccgcggaa ggcggcacac tgtttctcga cgagctcccc
      601 agcctctcgc tcggcgtcca agccaagctc ctcaaagcga tcgaggatcg cgccatccgc
      661 cgcgtcggcg gcacgcgcac cgtcgaaatc gacgctcgca tcgtcgccgc gacgaatgtc
      721 gatctccgcg cgctcgtcgc cgacggcaaa ttccgcgaag acctgctgca ccggctcgat
      781 ttatttcgcg tccgcatccc gccgctccgg gaacgcggcg acgacgtcat cgccctcgcc
      841 gaaaccctcg tcgcgcgttt cgcgaaaaaa tacggactcg ctccacgtcc cattcccgcc
      901 gccggccgcg aacggctgcg cgcccaccgc tggcccggta atgtccgcga actcatgcac
      961 gagatcgagc gctcgctcgt gttcgacgac gaccatctga ccttcgccgg cctcgccgcc
     1021 gccacccgcg cccaagccga caacaacagc cagctcctcc cgcccgactt ccattttccc
     1081 tccggcttct cgctcgaggc cacgatcgac gcattcgttc aacgcgcgct cgatcaaagc
     1141 gacggcaacg tctccgccgc cgcccgcctg ctcggcgtcc cgcgcgacta cgtccgctat
     1201 cgcataaaaa cgcgcaagcc cgccgccgcc gaactcagcg acgtctcccc gcacgccgac
     1261 tgatccaccg cggagggacg acctccgtgt cgtccgttcc tccgccaacc ctcgggctac
     1321 catccgttcc aaaaaatcgc tcgcgggtgg gaatcttttc ccaccgcgcc ggcgtatttt
     1381 gggaaatggt tcccgcttca cctcatcccg ctccggactc atgtccgtcg cttatccctg
     1441 ttaaatcgtc acttacgtcg attcttagcg gttggtatcg tggttgctaa agaagggccg
     1501 aacccagctt ccaaaaacaa ccatgacaaa aaacatcctc atatcgatcg ctcttgccgg
     1561 cggaatttcc gccggcgcca tcaacgtctc cgcgcaaaat gccgtcgacg ttcttaaaac
     1621 cgacgccggc gtctcagtgc ccgccgtgac gttcacgccc agcgagcgtc ccgccgcacc
     1681 gatcgaaggc aacgtggttc cgatcgatca cgtgatcgcg ctgccagact tcgtcgacgc
     1741 cgtgcccgtc ccgattacgc ggccgttgcc cgatttccaa atcgatacgt cccacgcctt
     1801 cgacggcaac gtcgcggtcc ggcccgacgt atccgtgaat ccgctcgatc cgacccagct
     1861 caacgtcgtc gaaatcggcg
//
LOCUS       DASPLT010000097         1412 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_267426, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000097 DASPLT010000000
VERSION     DASPLT010000097.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1412)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1412)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1412
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_267426"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..381
                     /locus_tag="VEA63_01035"
     CDS             <1..381
                     /locus_tag="VEA63_01035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucoamylase family protein"
                     /protein_id="HYD82591.1"
                     /translation="YDTWGLSAGINTGGGRPLPRDDNGTINVMASLASFPYTPDESMA
                     ALKHFYRDLGAKTWGVYGFYDGFNQTQNWFEPVYMALNQAPITVMIENHRTGLVWKHF
                     MANPEIAPALDAIGFEPDAAQRRR"
     gene            636..>1412
                     /locus_tag="VEA63_01040"
     CDS             636..>1412
                     /locus_tag="VEA63_01040"
                     /inference="COORDINATES: protein motif:HMM:NF013502.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheB"
                     /protein_id="HYD82592.1"
                     /translation="MARNNSSSSSNAPEPSNDTPPDRLAEPIDVEQPPRLAFAVVGIG
                     ASAGGLESFIQFFKTMPADSGMAFVLIQHLPPDHQSLIAEILSRHTTMPVHEIEDGMS
                     VEPDHIYVIRPGHTVTLRHGRLGLGPSLAKRGHGRPVDDFFRSLAEEQRQRAICVVMS
                     GMGSNGTTGAENVKAVGGLCIAEDPEAAKFPSMPRSLIDHGLADFVLRTEEIPEVLLR
                     YARHPYAALIEPPQPEPQQERQAVGEILAVLRARLRHDFSG"
BASE COUNT          266 a          501 c          385 g          260 t
ORIGIN      
        1 tacgacacct gggggctttc cgccggcatc aataccggcg gcggccgccc gcttccgcgc
       61 gacgacaacg gcacgatcaa cgtcatggcc tcgctcgcgt cattccccta cacgcccgac
      121 gaatcaatgg cggcgctgaa acatttctat cgcgacctcg gcgcgaaaac ctggggcgtt
      181 tacggcttct acgatggctt taaccaaact cagaattggt tcgagcccgt ctacatggcg
      241 ctcaaccagg cgccaatcac cgtgatgatc gagaaccatc gcaccggcct cgtctggaag
      301 catttcatgg cgaacccgga aatcgctccc gcgctcgacg ccatcggatt cgagcccgac
      361 gccgctcaac gccgacgctg aggagtcaca cccgcatgaa ggcgcgcaag cgcctgttac
      421 atggagccgc ggcgcctcgc cgcatttcgc tttaccaacg cgcgagggcg ccccgactcc
      481 atgattcatc ccggatagtt tatagatgag tttgtgtgcg gcacttcgca ccaccgaatg
      541 ggtccgtgct tgtcctttct ccctcgactc cgtctgcccc aagccggaaa accatttctg
      601 cgagggacgt cgatccttcc cagaatcctt tcttcatggc gcgaaacaac tcttccagtt
      661 cgtcgaatgc tcccgagccg agcaacgaca cgccgcctga tcgtctcgcg gaaccgatcg
      721 acgtcgagca accaccccgg ctcgcgtttg ccgtggtcgg catcggcgcg tccgctggag
      781 gactcgaatc gtttattcag ttcttcaaaa ccatgcccgc ggacagcggc atggcgttcg
      841 tgctgatcca gcacctgccg cccgatcacc aaagcttgat cgcggagatt ctcagccgtc
      901 acaccacgat gcccgtgcac gagatcgaag acgggatgtc ggtcgagccg gaccacattt
      961 acgtcattcg tcccggccac accgtcacac tccgacacgg ccgcttgggc ctcggccctt
     1021 cgctcgcgaa acgcggccac ggccggccgg tcgacgattt ctttcgctcc ctcgccgagg
     1081 agcaacgcca gcgcgcgatc tgcgttgtga tgagcgggat gggctccaac ggcacgaccg
     1141 gcgccgaaaa cgtcaaagcg gtcggcggtc tctgcatcgc cgaagatccc gaagccgcaa
     1201 agttcccgtc gatgccgcgc agcctcatcg atcacggcct cgccgatttc gtcctgcgca
     1261 cggaggaaat tccggaggtg ctgcttcgtt acgcgcgtca tccctacgcc gccctcatcg
     1321 agccaccgca gcccgagccg caacaggaac gccaggcggt cggtgaaatc ctcgccgtcc
     1381 tccgcgcacg cctccgtcac gatttcagcg gt
//
LOCUS       DASPLT010000098         1388 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_185555, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000098 DASPLT010000000
VERSION     DASPLT010000098.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1388)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1388)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1388
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_185555"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1237
                     /locus_tag="VEA63_01045"
     CDS             <1..1237
                     /locus_tag="VEA63_01045"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HYD82593.1"
                     /translation="GGTTGLALGIHRRDQFFSQSWDELSKAPGNWGFNGATAFLDFGG
                     KRSTDAIFGELVMYPTDTIEVQLAGRYEDTGDFDSTDPKIGVLWTPRDGLFIRASAGT
                     SFRQPGEIQVFGIGPGGASTDPIGGDTINARGLLVGNRNLKPETSDNWTAGITWDVTE
                     QFTAELNYWSVDFQDLITSEGSQAILLLDRADGFITDPRIILRDGAPNEVCEVTGRWS
                     GRPQDPRPAGCMSGFDVQLFRTTYINQAFQKTSGLDFNFSYDFDGFLDSEWSTRLLGT
                     WTHKYDMDVQGRVVDGVGSYNSATFGVANPEWRANAVLDWRRGNHMARGTYRYTSKLT
                     NDAPNANNMLTEEEDFATIDLTYGYNFADWNLDMTATVINVTDEEDPLRHGAQTTSTS
                     NIYEVRGRVFRVGFTWAME"
BASE COUNT          289 a          462 c          410 g          227 t
ORIGIN      
        1 cggcggcact accggcctcg cgctcggcat acatcgccgc gatcagttct tcagccagag
       61 ctgggacgaa ctgagcaagg cgccgggcaa ctggggcttc aacggcgcaa ccgcgtttct
      121 cgacttcggc ggcaagcgtt ccaccgacgc gatcttcggc gagctcgtga tgtatcccac
      181 cgacacgatc gaagtgcagc ttgccggccg ctacgaagac acaggcgact tcgattccac
      241 cgatccgaaa atcggcgtgc tgtggacgcc gcgcgatgga cttttcattc gcgcatcggc
      301 cggtacgtcg ttccgccagc ctggcgaaat ccaggtgttc ggcatcggcc cgggtggcgc
      361 ttccaccgat ccgatcggcg gcgacacgat caacgcgcgc ggtctgctcg tcggcaaccg
      421 caacctgaaa ccggaaacgt ccgataactg gaccgctggc attacgtggg acgtcaccga
      481 acagttcacc gcggaactga actactggag cgtcgacttc caggacctca tcacgtcgga
      541 aggttcgcag gcgatcctgc tgctcgaccg cgccgatggc ttcatcactg atccgcgcat
      601 catcctgcgc gacggcgcac cgaacgaagt ctgcgaagtg acgggaagat ggtcgggccg
      661 gccgcaggat ccgcgtcccg ccggctgcat gtcaggcttc gacgtgcagc tgttccgcac
      721 gacgtacatc aaccaggcct tccagaaaac cagcggcctc gacttcaatt tcagctacga
      781 cttcgacggc ttcctcgaca gcgaatggag cacgcgcctg ctcggcacct ggacgcacaa
      841 gtacgacatg gatgtacagg gccgcgtcgt cgacggcgtc ggcagctaca acagcgccac
      901 gttcggcgtc gccaaccccg aatggcgcgc gaatgccgtg ctcgactggc gccgcggcaa
      961 ccacatggcg cgtggtacgt accgttacac gtcgaaactg acgaacgatg cgccgaacgc
     1021 gaacaacatg ctgaccgaag aagaagactt cgcgacgatc gacctcacgt acggctacaa
     1081 cttcgccgac tggaacctcg acatgaccgc aacggtcatc aacgtcacgg acgaagaaga
     1141 cccgctgcgt catggcgcgc agacaacgtc gaccagcaac atctatgaag tgcgcggccg
     1201 tgtgttccgc gtgggcttca cctgggcgat ggagtaacac gccaaagcct cgccgcggaa
     1261 acgtggcggg gctacattcc cgtcattccc gcgcaggcgt gaatccagtc cgtcattccc
     1321 gcggaggcgg gaatccaatc cgtcattccc gcggaggcgg gaatccaatc cgtcattccc
     1381 gcgaaagc
//
LOCUS       DASPLT010000099         1354 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_206538, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000099 DASPLT010000000
VERSION     DASPLT010000099.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1354)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1354)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1354
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_206538"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1354)
                     /locus_tag="VEA63_01050"
     CDS             complement(<1..>1354)
                     /locus_tag="VEA63_01050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD82594.1"
                     /translation="GEILALTIQRLGPNNYQVTATESITLVRDIPNYDDSGLRNFALT
                     KRQVTGLLVVGTASDPIIYVTSSDPRIGGGSGGENDLNLDTNSGIVSRLTRSGSTWSK
                     VDLVRGLPRSEENHAPNGMQLDQVTNTLYLAQGGNTNAGSPSINFAYMCETALSSAVL
                     TIDLDTLDAMTVKTDAYGQKYLYNLPTLDDPNPSRTHNPDGSDSNDPFGGNDGLNQAK
                     LVAGGPVQIYSPGFRNPYDLVLTRTPGHEGRLYTFDNGANLGWGGYPQNEGAAGNTTN
                     QYVNGEPGQLNNRDSLHLISGPGYYGGHPNPIRANPAGAGWFHYDSTQPAGSEKIFSA
                     TPTTDWPPVPLAMANPVEGDFRLPGTANGALLTYPASTNGLAEYVATNFNGEMQGNLI
                     AASYDGTLLRIALNAAGTAVTNGVEPLASGFGNLPLDVTAPDPGHGAPFLGTIWVAHY
                     S"
BASE COUNT          227 a          345 c          517 g          265 t
ORIGIN      
        1 gaatagtgcg cgacccagat cgtgccgaga aaaggcgcgc cgtggccggg atcgggcgcc
       61 gtgacgtcga gcggaagatt gccgaaaccg ctggcgagcg gctcgacgcc gttggtgacg
      121 gccgtgccgg ccgcgttcaa cgcgatgcga aggagcgtgc cgtcgtaact cgcggcgatg
      181 aggttgccct gcatttcgcc gttgaagttg gtggcgacgt attcggcgag gccgttggtg
      241 gaggcgggat acgtgagcag ggcaccgttg gccgtgccgg ggaggcggaa atcgccttcg
      301 acgggattcg ccatcgcgag cgggacgggg ggccagtcgg tcgtcggcgt ggcggaaaaa
      361 attttctccg agccggcggg ttgcgtgctg tcgtaatgga accagccggc accggcggga
      421 ttcgcgcgaa tgggattcgg gtgaccgccg tagtaacccg ggccgctgat gaggtgaagg
      481 ctgtcgcggt tgttcaactg gccgggctcg ccgttgacat actgattggt ggtgttgccg
      541 gcggcgcctt cgttctgggg gtagccgccc caaccgagat tcgcgccatt gtcgaatgtg
      601 tagagccgac cctcgtgtcc gggcgtgcgc gtgagcacga gatcgtaggg attgcggaag
      661 ccgggggagt aaatctgcac aggaccgccg gcgacgagct tcgcctggtt gaggccgtcg
      721 ttgccgccga aaggatcgtt gctgtcgctg ccgtcgggat tgtgggtgcg cgagggattc
      781 gggtcgtcga gcgtcggaag gttgtagaga tatttctggc cgtaggcgtc ggttttaacc
      841 gtcatcgcat cgagcgtatc gagatcgatc gtgagcacgg cggaggagag cgcggtctcg
      901 cacatgtagg cgaaattgat cgaagggctg ccagcattgg tgttgccgcc ttgggcgaga
      961 taaagggtgt tggtgacctg atcgagctgc atgccgttgg gcgcgtggtt ttcttcggag
     1021 cgcggcaggc cgcggacgag atcgaccttg gaccaagtcg agccgctgcg cgtgaggcgc
     1081 gaaacgatgc cggagttcgt gtcgagattg agatcgttct cgccgccgct gccgccaccg
     1141 atgcgtggat cgctcgaagt gacgtagatg atcggatcgg aagcggtgcc gacgacaagg
     1201 agaccggtga cctgacgttt ggtgagcgca aaattgcgga ggccgctgtc gtcgtagttc
     1261 gggatgtcgc gcacgagcgt gatcgattcg gtcgcggtga cttgatagtt gttaggcccg
     1321 aggcgctgaa tcgtgagcgc gagaatctcg ccgt
//
LOCUS       DASPLT010000100         1282 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_185586, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000100 DASPLT010000000
VERSION     DASPLT010000100.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1282)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1282)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1282
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_185586"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(120..611)
                     /locus_tag="VEA63_01055"
     CDS             complement(120..611)
                     /locus_tag="VEA63_01055"
                     /inference="COORDINATES: protein motif:HMM:NF016985.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlgN"
                     /protein_id="HYD82595.1"
                     /translation="MNWQSIAENLRQELTEYGELLRLFEQQQESLFKRESDAVLQLGT
                     QIEDQAHILQDCRRQREKTVAAFAALVGQPAAATLRSLLPHVESDARPLLEALINEVN
                     VLLHRVRRASRQNHLMLGRTLELHQETLRQLRPDSFTKTYAATGRVSVAAAGGAPSLR
                     AAG"
     gene            complement(608..988)
                     /locus_tag="VEA63_01060"
     CDS             complement(608..988)
                     /locus_tag="VEA63_01060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD82596.1"
                     /translation="MNVVPINATTSAATNQIADLTVNAPTRSKPLTGSALRNASPEVQ
                     RKEVAAQFEAILVRQLLKPTMTSMVGSEGAATSVYGDLITETFATQLTRGQGLGLGRM
                     LEQQLTPRRPVSGDHLETAASTSP"
     gene            complement(992..>1282)
                     /locus_tag="VEA63_01065"
     CDS             complement(992..>1282)
                     /locus_tag="VEA63_01065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HYD82597.1"
                     /translation="RLSQVAIALGSLTITVSSNVGVSQPNAFAAGQTVAVPSTNTNVN
                     EVKGGFTVLNEPPTIERLAAALNALGVSTREMMAIFQSLKRSGALQAELVIN"
BASE COUNT          224 a          342 c          471 g          245 t
ORIGIN      
        1 gccggtcgtc tccacggcac ggctgtgcgc attgagcgcg ttgaccgagg cattgaggct
       61 ggcgaaaagg ccggacatat ccgttgaagt cgggagttga agatgacgcg cagtgaacct
      121 cagcccgcgg cgcggagcga aggggcgccg cccgcggcgg cgacggagac gcggccggtg
      181 gcggcatacg ttttcgtgaa ggaatccggg cgcaactggc gcagcgtttc ctgatgcagc
      241 tcgagcgtgc gaccgagcat aaggtgattc tggcggctcg cgcgacgaac ccgatgcagg
      301 agcacattga cctcgttgat caatgcttcg agcagcggac gcgcgtcgct ctcgacgtgg
      361 gggagcagcg aacgaagcgt cgcggcggcg ggctggccga ccaaggcggc gaaagccgcg
      421 acagtctttt cgcgctgacg acggcaatcc tgaaggatgt gcgcctggtc ctcgatctgc
      481 gtgccgagct ggaggacggc gtcagattcg cgcttgaaaa gagattcctg ctgttgctcg
      541 aacaggcgca ggagctcgcc gtattcggtg agttcctgac ggagattttc agcaatggat
      601 tgccagttca tggggaagtg gatgcagccg tttcgaggtg atcgccggaa acgggacggc
      661 gaggagtgag ttgttgttcg agcatccggc cgagtccgag gccttggccg cgggtcaact
      721 gggtcgcgaa agtttcggtg atcagatctc cgtagacgct cgtcgcggcg ccttcgctgc
      781 cgaccatcga ggtcatggtg ggcttcagga gctggcgaac gaggatggct tcgaattgag
      841 cggcgacttc cttgcgttgc acctcgggcg aggcgttgcg gagggcggag ccggtcagcg
      901 gtttggaacg cgtgggtgcg ttgacggtga gatctgcgat ctggttcgtc gcggccgacg
      961 tggtggcgtt gatgggaacg acgttcatgg cctagttgat cacgagttcg gcctgcagcg
     1021 cgccggaacg tttgaggctt tggaagatcg ccatcatttc acgcgttgaa acccccagcg
     1081 cgttgagcgc cgccgcgaga cgttcgatcg tgggtggctc gttgaggacg gtgaaaccac
     1141 ccttcacttc gttgacgttc gtgttcgtgc tcgggacggc gacagtctgg ccggcggcga
     1201 aagcgttggg ctggctgacc ccgacgttcg agctgaccgt gatcgtgagc gagccgagcg
     1261 cgatcgcgac ttgagaaaga cg
//
LOCUS       DASPLT010000101         1353 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_267478, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000101 DASPLT010000000
VERSION     DASPLT010000101.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1353)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1353)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1353
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_267478"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..358
                     /locus_tag="VEA63_01070"
     CDS             <1..358
                     /locus_tag="VEA63_01070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD82598.1"
                     /translation="DTDLATQVALGRVLFAAQDRSALARVIDRVTRLLPQRATLEPED
                     RVDLAALLTLAAREDLARPLLSETLEELDERALRRLTPGALISIIGLTQRLDIRWPDT
                     RLQTLASELLPPDGRI"
     gene            complement(408..>1353)
                     /gene="mgrA"
                     /locus_tag="VEA63_01075"
     CDS             complement(408..>1353)
                     /gene="mgrA"
                     /locus_tag="VEA63_01075"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047834 - D-threo-aldose 1-dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HYD82599.1"
                     /translation="SLGLWHNFGGSTPTENARAMCRRAFDLGITHFDLANNYGPPAGS
                     AETNFGEILRTDFAGLRDELVISTKAGYLMWPGPYGEWGSRKYLTASLDQSLKRMGLD
                     YVDIFYSHRVDPNTPLDETMGALDRALRSGKALYVGISSYNRRRTQEASALLKEFGDR
                     CLIHQPSYSMLNRWVETDGLLDTLESEGIGCIVFSPLAQGMLTNKYLGERPVDSRANT
                     NSSLRPEFLSEANLANIRTLNAIAQRRGQTLAQMAIAWVLRKPQVTSALIGASKPSQI
                     DDCVAALKNTSFSAEELAEIDRYAKDGDLNLWAASSKA"
BASE COUNT          240 a          453 c          392 g          268 t
ORIGIN      
        1 cgataccgat ctcgcaaccc aagtcgccct cggtcgcgtg ctcttcgccg cacaggatcg
       61 atcggcgctc gcgcgcgtca tcgatcgcgt gacacgcctg cttccccagc gagccacact
      121 cgagccggag gatcgcgtcg atctcgccgc cttgctcacg ctcgctgccc gcgaagattt
      181 ggcgcgtccg ctgctctctg aaacgttgga agaattagac gaacgcgccc tgcgccgcct
      241 gacgccagga gcgttaatca gcatcatcgg cctcacccag cgtcttgata tccgttggcc
      301 ggatacacgt ctgcaaacgc tcgcctccga gctcctgccg cccgacggac ggatctgatg
      361 cctcccgacg gtgttgccgc gacgtcacct tgcggtggtc gatcgcgcta tgccttgctc
      421 gacgccgccc agagattgag atcgccgtct ttcgcatagc gatcgatctc tgcgagctcc
      481 tcggcgctga aggacgtgtt cttcaacgcc gccacgcagt cgtcgatttg cgatggcttg
      541 ctcgcgccga tcaacgccga ggtgacttgc ggtttgcgca gcacccacgc gatcgccatc
      601 tgcgcgagcg tctgaccgcg acgctgcgcg atggcattca gcgtgcgaat gttcgcgagg
      661 ttggcttcgc tgaggaactc gggccgcagt gaactattgg tgttcgcgcg gctgtctaca
      721 ggtcgttcgc cgagatattt gttcgtcagc attccttgcg cgagcggcga aaacacgatg
      781 cagccgatgc cctccgactc cagcgtatcg agcaagccgt cggtctccac ccaacggttc
      841 aacatcgagt agctcggctg gtgaatgaga caacggtcgc cgaactcctt cagcaaagcg
      901 ctcgcctcct gcgtgcgccg gcggttgtag gacgagatgc cgacatacag cgccttgccg
      961 gagcgcagcg cgcgatcgag cgcgcccatc gtttcatcga gcggcgtgtt cgggtcgacg
     1021 cggtgcgaat aaaaaatatc cacgtaatcg agccccatgc gtttgaggct ctgatcgaga
     1081 ctcgcggtga gatatttccg gctgccccat tcgccgtaag gccccggcca catcaaataa
     1141 cccgccttcg tcgagatcac gagttcgtcg cgcagccccg cgaaatccgt gcgcaaaatc
     1201 tcaccgaagt tcgtttccgc cgatcccgcc ggtggtccgt agttgttcgc caaatcgaaa
     1261 tgcgtgatgc cgagatcgaa cgcgcgccga cacatcgcgc gcgcattttc cgtcggcgtg
     1321 ctcccgccga aattgtgcca cagcccgagc gag
//
LOCUS       DASPLT010000102         1248 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_22520, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000102 DASPLT010000000
VERSION     DASPLT010000102.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1248)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1248)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1248
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_22520"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..296
                     /locus_tag="VEA63_01080"
     CDS             <1..296
                     /locus_tag="VEA63_01080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD82600.1"
                     /translation="GDAAGFVDPFAGDGISLALRSGILAAEFAAAVVASRLTLAAALD
                     RYAREYDAQFRAIFRSAAFFRHGLNLPPVARATLLSALRVPSLARFAVRMTRG"
     gene            complement(308..835)
                     /locus_tag="VEA63_01085"
     CDS             complement(308..835)
                     /locus_tag="VEA63_01085"
                     /inference="COORDINATES: protein motif:HMM:NF022479.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2721 domain-containing protein"
                     /protein_id="HYD82601.1"
                     /translation="MSTPFNANHIAVLTAVAAPAILTNASSVLCLGIGNRIARVIDRT
                     RAVAAAEAQAGANTKLHDMLDHEQVLLRRRAKLLVYALRLGYTALGGFAAEALIAVIG
                     GVLSYSTYAHLSWGTGVVALAIGVLSVTAFVLSCAYMVRETMIALENLEHEAATVLHH
                     ASREDARLEQPKEVV"
     gene            complement(921..>1248)
                     /locus_tag="VEA63_01090"
     CDS             complement(921..>1248)
                     /locus_tag="VEA63_01090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012054698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD82602.1"
                     /translation="KAGKLPVPNFSAGGIATPADAAMMMQLGAESVFVGSGIFMKGGS
                     TPLDLKNAAEREEAISRAKAIVIATTHYNDPKVLAEVSEQMTGAMKGLAVAAIAEADL
                     LQTRGW"
BASE COUNT          187 a          433 c          388 g          240 t
ORIGIN      
        1 ccggagacgc ggcgggattt gtcgatccgt tcgcgggaga tggcatctcg ctggcactcc
       61 gcagcgggat tctggccgcc gagttcgccg ccgccgtggt cgcctcgcgg ctgacgctcg
      121 ccgccgcgct cgatcggtac gctcgcgagt atgacgcaca gtttcgcgcg atcttccgca
      181 gcgcagcgtt cttccgccat ggcctcaacc tgccgcctgt ggcgcgcgcg acactgctgt
      241 cggctctgcg tgttccttcc cttgcccgct tcgccgtccg catgactcga ggctgaacgg
      301 cgcgacccta cacaacctct ttcggttgct cgagtcgcgc gtcttcccgc gatgcgtggt
      361 gcagcacggt ggccgcctcg tgctcaaggt tctcgagggc gatcatcgtc tcgcgcacca
      421 tgtaggcgca gctcaggacg aaggcggtga ccgacaggac accgatcgca agcgccacca
      481 cgcctgtccc ccagctcagg tgggcgtagg tgctgtagct cagcactccg ccaatgacgg
      541 cgatcaaggc ctcggccgcg aagcctccga gcgccgtata gcccagccgc aaggcgtaca
      601 ccagcaactt cgcccttcgc cgcagcagca cctgttcgtg gtcgagcatg tcgtgcagct
      661 tcgtgttcgc gcccgcctgc gcctctgcgg ccgcgaccgc acgggtgcgg tcgattacgc
      721 gggcgatgcg gttgccgatc ccaaggcaca ggaccgagct ggcgttcgta agaatcgccg
      781 gcgcggcgac ggccgtcagc acggcgatat ggttcgcgtt gaatggagtg ctcattccct
      841 cttcctaaac ggcgcaggcg cagccgaagc tgcgcctgaa cgttgcatcg taatcttccg
      901 gtgtagtcgc gtcttgcgcc ttaccagcca cgagtctgaa gcaggtccgc ctccgcgatg
      961 gccgcgacgg ccaaaccctt catcgctccg gtcatctgct cgctgacttc ggccagcacc
     1021 ttcggatcgt tgtagtgcgt cgtggcgatc acgatggcct tggcacgcga gatcgcttcc
     1081 tcgcgctctg cggcgttctt tagatcaagc ggagtcgatc cacccttcat gaagatgccc
     1141 gagcccacga aaaccgattc ggctccaagc tgcatcatca tcgcggcgtc cgccggcgtg
     1201 gcgatgcctc cggccgagaa gttcgggacg gggagcttgc ctgccttg
//
LOCUS       DASPLT010000103         1136 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_288888, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000103 DASPLT010000000
VERSION     DASPLT010000103.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1136)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1136)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1136
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_288888"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            120..>1136
                     /locus_tag="VEA63_01095"
     CDS             120..>1136
                     /locus_tag="VEA63_01095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD82603.1"
                     /translation="MKRGRSAAEGPSAIELIEEAVHVLRRAPMGALAVYLTGTAPFVF
                     GIVFFWARTTWFQPGEAALAGGALVLVGLFVMMKTAQAEFCARLLAFRMGTDAPPLTW
                     QRVLRVAVAQSRVQPWGLFVLPVALVLSVPFGWAYAFFQNATIAGDKEPLGAIAARQA
                     KLWPAQNHLGLLFICVLGGAAWINIAAAFWVVPWLANRLLGLQNLFGFSGWWFLNTTF
                     LVSTLAMTWLAIDPLVKTFYTLRVFYGEARRNGEDIQVELRLVQRSRTGGVRAALALA
                     VILPWWPVNRVTADERRSGETVSTVQPAALDQAIDEALNDSVFSWRLRPVPERTATEA
                     DDGLF"
BASE COUNT          168 a          311 c          413 g          244 t
ORIGIN      
        1 gcggcgttgg ggctgctggt tgccggtttg acgttttacg gtttcgggcg cgtgctgcgc
       61 aaaatcccgc cggagttgca tcggttctcc gagaattggc cgaagccaaa ggcaggtcga
      121 tgaagcgcgg acgctctgca gccgaaggtc cgtcggcgat cgaattgatc gaagaggccg
      181 tgcatgtgct gcgacgtgct ccgatggggg cgctggcggt ttatctgacg ggcacggcgc
      241 cgtttgtttt cggcatcgtg tttttctggg cgaggacgac gtggtttcag cctggggagg
      301 cagcgctcgc cgggggcgcg ctggtgctgg tcggactatt cgtgatgatg aaaacggcgc
      361 aggcggagtt ttgcgcgcgc ctgctggcct ttcggatggg cacggatgct ccgccgctca
      421 cgtggcaacg cgtccttcgc gtggcggtgg cgcaatcgcg ggtgcagccc tgggggctgt
      481 tcgtgctgcc ggtggcgttg gtgttgagcg tgccgtttgg atgggcgtat gcgtttttcc
      541 agaatgcgac gattgctggc gacaaagagc cgctcggggc gatcgcggcg cgacaggcga
      601 aattgtggcc ggcgcagaat cacctcggat tactgttcat ctgcgtcctg ggtggcgccg
      661 catggatcaa tatcgcggcc gcattctggg tcgtgccctg gctggcgaac cggctgctcg
      721 ggttgcaaaa tctctttggt ttttcgggtt ggtggtttct gaacacgacg tttctcgttt
      781 cgacgctggc gatgacgtgg ctcgcgatcg atccgctcgt gaagaccttc tatacccttc
      841 gcgtgttcta cggcgaagcc cgccgcaacg gagaggacat ccaggtcgag ttgcgacttg
      901 tgcaacgcag ccgaaccggc ggcgtgcggg cggcgctggc gttggcggtc atcctgccct
      961 ggtggccggt gaaccgagtc acggcggacg aacgacgaag cggtgagact gtttccacgg
     1021 tgcaaccggc ggcactcgat caggcgatcg atgaagcact gaacgacagc gtgttttcgt
     1081 ggcgcctgcg cccggtcccg gaacgaaccg cgaccgaggc cgacgacggg ctgttc
//
LOCUS       DASPLT010000104         1876 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_206625, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000104 DASPLT010000000
VERSION     DASPLT010000104.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1876)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1876)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1876
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_206625"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1176
                     /locus_tag="VEA63_01100"
     CDS             <1..1176
                     /locus_tag="VEA63_01100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HYD82604.1"
                     /translation="LGVKKRPGAGSDFWALRDVSFDVQQGEVVGLIGRNGAGKSTLLK
                     ILSRITEPTLGEIRLRGRVASLLEVGTGFHPELSGYENIFLNGAILGMSRAEIRSKFD
                     EIVEFSEIGKFINTPVKRYSSGMYVRLAFAVAAHLEPEILIIDEVLAVGDVAFQRKCL
                     GKVREISRGQNRTVMFVSHNMNAVENICTRGVLMKQGQLVREGSDVREIVRHYIAEHA
                     QMASGASWENPGDAWKNDYFRVDRFYLTDPNGDVITHPVANDTPVTVVIEGEVLKPDS
                     SLQIGYGLFTDEMDLLYISTNLDQPEATWTELQRGSVRLTSRIPARLINEGEYRVGLF
                     LSLYRRKWFCEPGVKGPTVAFEIRGGLSDSPCLVTRRPGYFAPVLGWDVRTGVELIA"
     gene            1180..>1876
                     /locus_tag="VEA63_01105"
     CDS             1180..>1876
                     /locus_tag="VEA63_01105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD82605.1"
                     /translation="MSAHAPVALFVYNRPDHTRQTLEAFARNRLAAETPLYVFADGPR
                     DDATSADRERIAATRALVRSRAWTKQINFIDSDHNRGLANSILGGVRHVLAEHDRIIV
                     LEDDIVTSPGFLEFMNSGLELYADEPRVMHLSGYMYPLGLQNKDTVFLRILSCWGWAT
                     WARAWQHYDDDLETHLARIDTPHRVRKFNIEGHADFHRQLLENRSGRIRSWAVRWYAS
                     WLARDGLSLFPTQS"
BASE COUNT          340 a          662 c          520 g          354 t
ORIGIN      
        1 ctcggcgtga agaagcgacc gggtgccggt tccgattttt gggcgctgcg cgacgtcagc
       61 ttcgacgtgc agcaaggcga agtcgtggga ctcatcggac gcaacggcgc cggcaagagc
      121 acgcttctga agatcctcag ccgcatcacc gaacccacgc tcggcgaaat ccgcctgcgc
      181 ggtcgcgtcg cgagcctcct cgaggtcggc accggcttcc atcccgagct cagcggctac
      241 gaaaatattt tcctcaacgg cgccatcctc ggcatgtcgc gcgccgagat ccgttccaag
      301 ttcgacgaga tcgtcgagtt ctccgagatc ggaaaattca tcaacacgcc cgtgaagcgt
      361 tactcgagcg gcatgtatgt gcggctcgcg ttcgccgtcg ctgcgcacct cgagcctgaa
      421 atcttgatca tcgacgaggt gctcgccgtc ggcgatgtcg cgttccagcg caaatgcctc
      481 ggcaaggtcc gcgagatttc ccgcggccag aaccgcacgg tcatgttcgt cagccacaat
      541 atgaacgcgg ttgaaaacat ctgcacgcgc ggcgtcctga tgaagcaggg ccaactcgtt
      601 cgcgagggct ctgacgtgcg tgaaatcgtt cgccactaca tcgccgagca cgcgcaaatg
      661 gcgtccggcg cgtcgtggga aaatcccggc gatgcgtgga aaaacgacta cttccgcgtc
      721 gaccgtttct atctcaccga tccgaacggc gatgtcatca cgcacccggt tgccaatgac
      781 acgccggtca cggtggtgat cgagggcgag gtgctgaagc ccgactcgag tctccaaatc
      841 ggctacggtc ttttcaccga cgagatggac ctgctctaca tcagcaccaa tctcgaccag
      901 cccgaggcca cttggaccga gttgcaacgc ggctccgtcc gcctcacctc gcgcatcccc
      961 gcccgcctca tcaacgaggg cgaataccgc gtcggccttt ttctcagtct ctatcgccgc
     1021 aaatggttct gcgaacccgg cgtcaaaggc ccgacggtcg ccttcgaaat ccgcggcggc
     1081 ctcagcgatt caccctgcct cgtcactcgc cggcccggct atttcgcgcc ggtcctcggc
     1141 tgggacgttc gcaccggtgt cgagctcatc gcctgaaccg tgtccgctca cgctcccgtc
     1201 gctctcttcg tctacaaccg gcctgaccac acgcgtcaga ccttggaggc gttcgcgcgc
     1261 aaccgcctcg ccgccgaaac gcctctctac gttttcgccg acggtcctcg cgacgacgcc
     1321 acgtccgccg atcgcgaacg catcgcggcc acgcgcgcac ttgtccgcag ccgcgcgtgg
     1381 actaaacaaa tcaacttcat cgactccgac cacaatcgcg gtctcgccaa ctccattctc
     1441 ggcggcgtgc gacacgtgct cgcggaacac gaccgcatca tcgtgctcga ggacgacatc
     1501 gtcacctcgc ccggctttct cgagttcatg aactccggtc tcgaactcta cgccgacgag
     1561 ccgcgcgtga tgcatctctc cggctacatg tatccgctcg gtctccaaaa caaggatacg
     1621 gtgttcctgc gcatcctctc ctgctggggc tgggcgacgt gggcgcgcgc gtggcagcac
     1681 tacgacgacg atctcgaaac ccacctcgcc cgcatcgaca cgccgcatcg cgtgcgcaaa
     1741 ttcaacatcg agggtcacgc cgatttccat cgccagctgc tcgaaaatcg cagcggccgc
     1801 atccgctcct gggccgttcg ctggtatgcg tcgtggctcg cgcgcgatgg cctttcactt
     1861 tttccgacgc aatcgc
//
LOCUS       DASPLT010000105         2039 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_288900, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000105 DASPLT010000000
VERSION     DASPLT010000105.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 2039)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2039)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2039
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_288900"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(522..1862)
                     /locus_tag="VEA63_01110"
     CDS             complement(522..1862)
                     /locus_tag="VEA63_01110"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HYD82606.1"
                     /translation="MANKKRFVLVGTGARAVNFITQFALRFREHVELAALCDLSPTRM
                     AYCNALLAGELGYHAVPTYRADQFEQMLDEQRAEVVIVTTTDGTHDDYIVRALRRGCD
                     VVTEKPMTVDAAKCRRIFAAVKETGKNVQVAFNVRWAAHRTKIRELIASGVVGRVTSV
                     HLEYLLNTSHGADYYRRWHATMAESGGLLVHKSTHHFDLVNWWIDAIPEQVFASGRLD
                     YYGRANAVARGQEALTKYARYTGEPAAANDPFALDLSQHENFRRLYLDAEAESGYLRD
                     RNVFRDGIDIYDNMSLAVRYRTGVLLNYSLLSFSSREGFHVAINGDRGRIEYDEYQPT
                     HVLSDHEGAPPPAAGDRSLERIRVYPHFAPSYEVEVQRLEGEHDGSDLLLAEQIYSPN
                     PPVDRFGRKAGYEQGAASILIGIAANESIASNRPVAIDDLVSLKPGATRLSELI"
BASE COUNT          422 a          618 c          602 g          397 t
ORIGIN      
        1 gccttcgatc gaaaattcgt tggcgtaaac catcaatcga atcatcgact gacggtcgtc
       61 gggatcgccg ccgatatccg tgagcaccag caggcgcggt cgctcgtccg ccacgcaacc
      121 gaccgcgagc agcaaggcgc agacaacgag tttccaggtc atgatttatt cgggaaattg
      181 aacctcgagc aattcagtag gaaaccaccg atgggcacga atcgacacga atttagtgaa
      241 gacaaacgtg tcgcatcagc ttcgttaacc acgaggccgt atccgtgttt gtcggtgtcc
      301 atccgtggtt caattgcagg ttccggctta gccggactcc tacagaagag aaaccacgaa
      361 tggacacgaa tgaacacgaa ttcagaggag gcagaggaaa gctttgggat ctatcgtgtg
      421 catcaccgtt tggtgttccg atccgattct gcatccgaac ttggtttcat tcgtgtcgat
      481 tcgtgtccat tcgtggttca aatgaattat tccggctgag tttagatgag ttcgctcaat
      541 cgcgtggcgc ccggcttcag gctgacaagg tcatcgatcg cgaccggccg gttgctcgcg
      601 atcgattcgt tcgccgcgat gccgataagg atcgacgccg ctccctgctc gtagcccgcc
      661 ttgcgcccga agcgatccac gggcggattg ggcgagtaga tttgctcggc cagcaataaa
      721 tcggacccgt cgtgctcgcc ctcgagccgt tgcacctcga cttcgtagct cggggcaaaa
      781 tgcggataga cgcggattcg ctcgagcgac cgatcccccg ccgcgggcgg cggcgctcct
      841 tcgtggtcgg acagcacatg cgtcggctga tactcgtcat attcgatccg gccgcgatcg
      901 ccgttgatcg ccacgtgaaa tccttcgcgc gacgagaagc tcaaaagcga atagttgagc
      961 agcacgccgg tgcgatagcg gacggccagc gacatgttgt cgtagatatc gatgccgtcg
     1021 cgaaacacgt ttcgatcgcg caggtagccg ctctccgcct cggcatcgag atagagccgc
     1081 ctgaaatttt cgtgctggct caggtcgagc gcaaacggat cgttcgccgc ggccggttcg
     1141 ccggtgtagc gcgcatactt cgtcaacgct tcctggccgc gcgcgacggc gttggcgcgg
     1201 ccatagtaat cgagccgacc cgaggcgaag acctgctcgg gaatcgcatc gatccaccaa
     1261 ttcaccagat cgaaatgatg cgtgctcttg tgcacgagca atccacccga ctcggccatc
     1321 gtcgcgtgcc agcggcggta gtaatcggcg ccgtgggagg tattgagcag gtattcgaga
     1381 tgcacgctcg tcacccgacc caccacgccc gaagcgatga gttcgcgaat cttcgtgcgg
     1441 tgcgccgccc agcgcacgtt gaacgccacc tgaacgttct tcccggtctc cttcacggcc
     1501 gcgaagattc gccggcattt cgcggcgtcg accgtcatcg gtttttcggt gacgacatcg
     1561 cagccccgtc gcaaggcgcg cacgatatag tcgtcgtgcg tcccatcggt tgtggtgacg
     1621 atgacgacct cggcgcgctg ctcgtcgagc atctgctcga actggtccgc acgataagtc
     1681 ggcacggcat ggtagccgag ttccccggcg agcagcgcgt tgcagtaagc catgcgcgtc
     1741 gggctgagat cgcacagcgc ggcgagttcg acgtgttcgc gaaaccgcag cgcaaactgc
     1801 gtgatgaaat tcaccgccct cgcgcccgtg ccgacgagga caaaccgttt tttgttggcc
     1861 atgaaaaagg agatgacgtt cgaggtgacg ccggtcgcga ttcgcctccg ccggcacccg
     1921 ctccgacaca aacgcggcac ggctcataaa agccggagcc gcgcttcgat cggaacggaa
     1981 cggcaacgaa gcgcatcgac cacgaagcaa aacgacgcgc tcgcctgagc ataaaaata
//
LOCUS       DASPLT010000106         1653 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_247226, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000106 DASPLT010000000
VERSION     DASPLT010000106.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1653)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1653)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1653
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_247226"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..569
                     /locus_tag="VEA63_01115"
     CDS             <1..569
                     /locus_tag="VEA63_01115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YdeI/OmpD-associated family protein"
                     /protein_id="HYD82607.1"
                     /translation="PIRPRFFPAPAAFRRWLEQHHSAANELWVGFYKKSSGRGGMVYA
                     EAVDEALCFGWIDGLVKSIDSDSYMQRFTPRKPRSIWSKINVGHIARLTAAGRMTPAG
                     LAAFEARTAAKTGVYSFEQEPQQFPAAFEKTFRANRRAWTFWTSQPPGYKRTALWWVI
                     SAKQASARERRLAQLIAVSADHRRLQGK"
     gene            722..>1653
                     /locus_tag="VEA63_01120"
     CDS             722..>1653
                     /locus_tag="VEA63_01120"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HYD82608.1"
                     /translation="MTFARRLALASLLTLSALAPVSPLTAASAKKSAPAPAYKGAVIM
                     DAATGNVLFEDHQGDPSPPASMTKLMTFLVLHDKLASGALTLQTPVIVTGDDSRIGGT
                     QVWLKEKEEFTVEELLYAMMIQSANDAAYALARTAAGSVPAFVELMNEKARELGMTNT
                     TFRTPHGLPPSNRKFADSDLTTPHDFALLARELLLKTDVLHYASVKTRPFGDGQRAQP
                     VVMNNHNHLLGKIAGVDGLKTGFTNGAGFCLTATAERNGRRIIVVTMGSPDRKLRDIK
                     VAELLERGFVALPANSPAFTSEAPRENSSADNGLA"
BASE COUNT          315 a          642 c          412 g          284 t
ORIGIN      
        1 cccccattcg cccccgcttc ttccccgcac ccgccgcatt tcgcaggtgg ctcgagcaac
       61 accacagcgc cgccaacgag ctgtgggttg gcttctacaa aaaatcctcc ggccgcggcg
      121 gtatggttta cgccgaagcc gtggacgaag ctctctgctt cggctggatc gatggtctcg
      181 tgaaatccat cgactccgat agctacatgc agcgtttcac gccgcgcaaa ccgcgcagca
      241 tctggagcaa aatcaacgtc ggtcatatcg cccgccttac tgccgccggc cgaatgactc
      301 ccgccggcct cgccgcgttc gaagcacgca ccgccgccaa aacgggcgtc tactccttcg
      361 aacaggagcc gcagcaattt cccgcggcct tcgaaaaaac cttccgcgcc aaccgccgcg
      421 cttggacgtt ttggacgtct cagccgcccg gatacaaacg caccgcgctc tggtgggtga
      481 tcagcgcgaa gcaggcgtcc gcccgcgaac gccgcctcgc ccaactcatc gccgtctccg
      541 ccgaccaccg gcgcctccag ggcaagtagg gtgggcttca gcccgccttc cgataccgcg
      601 cgcgcattct gccgccgcat ccacagcgcg cgccgcctgc gccgcgtatt tcaccaaact
      661 caaacattcg cgcaccgatt tcctctcgct cgcccacaaa aaaattctct ctcttcgatc
      721 aatgactttc gcccgccgcc tcgcgctcgc ttccctcctc acgctttctg ccctcgcgcc
      781 cgtttcgccg ctcaccgccg cctccgccaa gaagtccgcg cccgccccag cctacaaagg
      841 cgcggtcatc atggacgccg cgaccggcaa cgttcttttc gaagatcatc aaggcgaccc
      901 gagcccgccc gccagcatga cgaagctcat gacgtttctc gtgttgcacg ataaactcgc
      961 cagcggcgcg ctcacgctgc aaacgcccgt catcgtcacc ggcgacgact ctcgcatcgg
     1021 cggcacccaa gtctggttga aagaaaaaga ggagttcacc gtcgaggaac tcctctacgc
     1081 gatgatgatc cagtccgcga acgatgcggc ttacgccctc gcgcgcaccg ccgccggctc
     1141 cgttcccgcc ttcgtcgagc tcatgaacga aaaagcccgc gagctcggca tgaccaacac
     1201 cacctttcgc acgccgcacg gccttccgcc gtcgaatcgc aaattcgccg acagcgatct
     1261 cacgacgccg cacgatttcg ccctgctcgc gcgcgaactt ctcctgaaaa ccgatgtgct
     1321 ccactacgct tcagtcaaaa cgcgcccctt cggcgacggt cagcgcgccc agcctgtggt
     1381 gatgaacaac cacaatcacc ttctcggcaa aatcgccggt gtcgacgggc tgaaaaccgg
     1441 tttcaccaac ggcgccggct tctgcctcac cgcgaccgcc gagcgcaacg gccgccgcat
     1501 catcgtcgtc accatgggca gcccggatcg aaaactccgc gacatcaaag tcgccgagtt
     1561 gctcgaacgc ggctttgtcg cgcttcccgc caacagcccc gccttcacca gcgaggctcc
     1621 ccgcgaaaac tcttctgccg acaacggtct cgc
//
LOCUS       DASPLT010000107         3286 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_144962, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000107 DASPLT010000000
VERSION     DASPLT010000107.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 3286)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3286)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3286
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_144962"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..553
                     /locus_tag="VEA63_01125"
     CDS             <1..553
                     /locus_tag="VEA63_01125"
                     /inference="COORDINATES: protein motif:HMM:NF015436.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transporter associated domain-containing
                     protein"
                     /protein_id="HYD82609.1"
                     /translation="EEPDNIVGVLHARDLLRAISEHGRDGFDVRTVMRQPWFTPMTTS
                     AEDQLAEFLRRREHFAIVVDEYGALMGLVTLEDILEEIVGDIKDEHDIAPTGVRPQPD
                     GSVNVDGAVPIRDLNRVMNWDLPDEEAVTVAGLVIHEAQAIPEPGQRFAFHGYRFQVL
                     RRQRNQLTALRVEREVEERMEGG"
     gene            580..1995
                     /locus_tag="VEA63_01130"
     CDS             580..1995
                     /locus_tag="VEA63_01130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD82610.1"
                     /translation="MTDENTQRRQVPIFVRLVRDDTLGIHSGFSAGRDPLELARRPRK
                     QKDPDENPKPEEEGAKNAASRFDDAVASFSEDMLAFLVAADRHFALDRMVSAIEVEAG
                     LKPKLEAAGRKIKERPGYTLYAIAEEHLHIVDRAAIAFHTARVFTRFMPKMLLAALIA
                     RYDAFLAQLIEALFYSKPEMARGLASKISFEEILDLGSIEDARRFMVEREAENVLRNS
                     HDEQLKWFEEVTKLKTRKHITNYAAFIELCERRNLLVHTDGRVSSTYLNNLARHGIPS
                     DELKVGDQLHVSRQYYGDAVAKLIEVGTKLALSCWEKFAPGEREKLESRLIHLTFELI
                     VHRHYKLAVNVLAYVLDNKKTWADDDNRLICLMNYANAMKLSGDSEEAARILSTDDWS
                     TKDRLFRVCVAAVRDDVEECVLQMKALGVSKQLTASAYKFWPVFKNVKEDARFVSAVK
                     DIYGDDALLLQNQEVLSEEKS"
     gene            complement(2038..2280)
                     /locus_tag="VEA63_01135"
     CDS             complement(2038..2280)
                     /locus_tag="VEA63_01135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD82611.1"
                     /translation="MKITIGAARASGAVGLLLYCANYFETPGGCRHDSKMTMLLAVAL
                     FGEEKRLDELPFRCSVCGGREVDVRPWFASAPRKGR"
     gene            2594..2941
                     /locus_tag="VEA63_01140"
     CDS             2594..2941
                     /locus_tag="VEA63_01140"
                     /inference="COORDINATES: protein motif:HMM:NF016370.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease domain-containing protein"
                     /protein_id="HYD82612.1"
                     /translation="MRNERSIRRAREMRAEPTSAEEKLWRMLRGRRYREAKFRRQHAI
                     GKYVVDFACVASRLVIEVDGPSHDDEEQKQFDATRTADLERWGWRVARVPNAQIHAGG
                     DGLYDFLDNALKG"
BASE COUNT          720 a         1008 c          941 g          617 t
ORIGIN      
        1 tgaagagccc gacaatatcg tcggcgtcct gcacgcgcgc gatctcctgc gcgccatttc
       61 cgaacacggc cgcgacggct tcgacgtgcg cacggtgatg cgccagccct ggttcacgcc
      121 gatgaccacc agcgccgaag atcagctcgc cgaattcctg cgccgccgcg aacatttcgc
      181 catcgtcgtc gacgaatacg gcgcgctgat gggcctcgtc accctggaag acatcctcga
      241 agaaatagtc ggcgacatca aagacgagca cgacatcgcc cccacgggcg tccgccccca
      301 acccgacggc agcgtcaacg tcgacggcgc cgtccccatc cgcgacctga accgcgtgat
      361 gaactgggac ttgcccgacg aagaagccgt gacggtggcg gggctggtga tccacgaagc
      421 gcaagcgatt ccggagccgg ggcaacgctt cgcgtttcac ggctatcggt ttcaagtgct
      481 gagaaggcag cgcaaccagc tcacggcgct acgggtggag cgggaagtag aggagcggat
      541 ggaggggggg tagtatctcg cgattcgcgg ggttcttaca tgaccgacga aaatacgcag
      601 agacgtcagg tgccgatctt tgtgcggctg gtccgagatg acactctagg tattcactcg
      661 ggtttttccg cgggtcgtga tccccttgaa ctcgcccgcc ggcctcggaa gcaaaaagac
      721 ccagacgaga atccaaagcc cgaagaagaa ggcgcaaaga acgccgcttc ccggttcgac
      781 gatgcagtgg cctcattctc cgaggatatg ctcgcgtttt tggttgccgc agatcggcac
      841 tttgcacttg atcggatggt tagtgccatt gaggtcgaag cggggctgaa accaaagttg
      901 gaagcggcgg gccgtaagat taaggaacgg cctggctaca cactttatgc tatcgccgag
      961 gagcatttgc acatcgttga tcgcgcagcg atcgcgttcc ataccgcccg cgtcttcacg
     1021 cggttcatgc ctaaaatgct cctggcagct ctgattgccc gctacgacgc atttttggcg
     1081 caactgatcg aagcgctctt ctattcgaag ccggagatgg cgcgagggtt ggcaagcaaa
     1141 atatcattcg aagaaattct tgatctcggt tctatcgaag atgctcggcg ctttatggtt
     1201 gagcgggagg ccgagaatgt cctccgtaat agccatgacg aacaactaaa gtggttcgag
     1261 gaggtgacca aactcaaaac gcgcaaacac atcactaact acgcggcgtt catcgaactt
     1321 tgcgaacgcc gcaacctgct ggttcatacg gatggtcgag tatcatcaac gtacctcaat
     1381 aatttagcgc gccacggtat tccttccgac gagctcaaag ttggtgatca gttgcatgtg
     1441 tcacgccagt attatggtga cgccgttgca aagctgatcg aagttggcac caagctagcg
     1501 ctttcttgtt gggaaaaatt cgcgccgggt gaacgagaga agcttgaatc ccggctaatc
     1561 cacctgactt ttgaactgat cgtgcatcgc cactataaac tggcggtcaa tgtcctggca
     1621 tatgtcctcg acaacaagaa aacttgggcc gacgacgata accgactgat ctgcctgatg
     1681 aactatgcca atgccatgaa gctgagcggt gactccgagg aggccgccag aattctttcc
     1741 accgacgatt ggtctacgaa ggaccggcta tttcgcgtgt gcgtggccgc tgtgcgcgac
     1801 gatgttgagg agtgcgtgct gcagatgaaa gcgctcggag tctccaagca gcttaccgct
     1861 tcagcctata agttttggcc tgtttttaag aatgttaaag aagacgcgcg gtttgtgtcc
     1921 gcggtgaagg acatctacgg cgacgacgct ctgctgcttc agaaccaaga agttttgagc
     1981 gaagaaaaat cataagcgtt gagtgccgtc tgctcgctca ttgtgcgcaa cgctcactta
     2041 ccgccccttc cgcggcgcac tcgcaaacca cggccgcacg tccacctcgc gcccgccgca
     2101 taccgagcat ctgaacggca gctcatccag ccgcttttcc tcgccgaaca gcgcgaccgc
     2161 cagcagcatc gtcatcttcg aatcgtgtct gcacccaccc ggcgtctcga aataattggc
     2221 gcaatagagc agcagcccga cagcgccgct tgcgcgcgcc gcgccgatcg taatcttcac
     2281 acccacttgc cgcttccttc tgaagcgacc ccatgcgcgc cacgatgtaa gcgcgcgccc
     2341 gcgccgcgcc agcgcggtaa tcgcgagcag atgtgtgcaa cagctttcct tcgcccccgc
     2401 ggggggggga gaaggtggtc gcgactcgtc cgccgtagct ttagcgaagg cggaagcgac
     2461 cggatgaggg ggcgagcgtc aggaggaggc gccgctctcg gcaccgcacc atacctgagc
     2521 ctcgccccct cacccgattt gcgtatgcaa atcgacctct ccccctcgcg ggggagaggt
     2581 gtaagatgga gccatgcgca acgaacgctc gatccgccgc gcacgcgaaa tgcgcgctga
     2641 gccgaccagc gcggaagaaa agctctggcg catgctgcgc gggcgccgct atcgcgaagc
     2701 caagtttcgc cgtcagcacg ccatcggcaa atacgtcgtc gacttcgcct gcgtcgcatc
     2761 acgtctcgtc atcgaagtcg acggtccctc gcacgacgac gaagaacaaa agcaattcga
     2821 cgctacccgc accgccgatc tcgaacggtg gggctggcgc gtcgcccgcg tccccaacgc
     2881 ccaaatccac gccggtggcg acggcctcta cgacttcctc gacaacgccc taaaaggtta
     2941 gccccacctc gcccccgcga gggggagagg tcgctcgcga atgcgagcgg gtgagggggc
     3001 gagcatcaga attgggcgcc gtcagcaaca ccgcgcccca ttctgaacct cgccccctca
     3061 tccgtccgct tcgcggacac cttctccccc tcgcgggggc gaagggaaac tatcaatcgc
     3121 ttttccgcgc taaagcattg aaaccgcaca caacaaaaga aatctatccg ccccccaaaa
     3181 agctgaggga taatcctcct accgtttagc aggagagcag tcggccgctg ttcgaaaccg
     3241 atgcgagaac ggtgtgatcg agcgtgatgc gtagaggctt ctcaaa
//
LOCUS       DASPLT010000108         1639 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_206660, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000108 DASPLT010000000
VERSION     DASPLT010000108.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1639)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1639)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1639
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_206660"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..230
                     /locus_tag="VEA63_01145"
     CDS             <1..230
                     /locus_tag="VEA63_01145"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HYD82613.1"
                     /translation="PDLVITDLQLDESDGLATVEKLKELLPQTPVILLTGVLIEPSVA
                     AATVGTKVAAYIEKTAPLTTLIAAIEQAIGH"
     gene            complement(318..551)
                     /locus_tag="VEA63_01150"
     CDS             complement(318..551)
                     /locus_tag="VEA63_01150"
                     /inference="COORDINATES: protein motif:HMM:NF022969.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexameric tyrosine-coordinated heme protein"
                     /protein_id="HYD82614.1"
                     /translation="MIAPSLPFEFPASLQTATPAEGQALAHQLATFVLREGSPTRATM
                     AEMLRRPDDAPPIALVAAIYFHAISIANRGWRS"
     gene            complement(548..>1639)
                     /locus_tag="VEA63_01155"
     CDS             complement(548..>1639)
                     /locus_tag="VEA63_01155"
                     /EC_number="1.11.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009513726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase"
                     /protein_id="HYD82615.1"
                     /translation="FPDFIHTQKRDPQTNLRNNTAAWDFWSLSPESLHQVTILMSDRG
                     IPKNLRQQHGFGSHTYSFINVIGERYWVKFHFKSLQGIATYTDAEAAAIVGQNRESSQ
                     ADLFHAIADGNFPRWRFCVQIMPEADADSYKVNPFDLTKVWPHADYPLIDIGVLELNR
                     NPQNYHAEVEQAAFSPANVVPGIGHSPDKVLQSRIFSYADAHRYRLGVNHQHLPVNAP
                     RCPIFNYQRDGAMRFDGNGGSAVNYEPNSFNGPKDCPALNEPPLKISGDVDRYNHRDG
                     NDDYTQPGNLFRLFTPDQKERLFGNIARHMAGVPREIQLRQICHFFRADPAYGLGVAK
                     ALNLDIAEFVAKASSSSSANGHVAPALQS"
BASE COUNT          291 a          398 c          638 g          312 t
ORIGIN      
        1 cgccggatct cgtgattacc gatctgcaac tcgacgagag cgacggtctg gcgacggtcg
       61 aaaagttgaa ggagctgctg ccacaaacgc cggtgattct gctcaccggc gtgctcatcg
      121 aaccttcggt ggcggcggcg acggtgggga cgaaggtcgc ggcgtatatc gagaaaaccg
      181 cgccgttgac gacgttgatc gcggcgatcg agcaagcgat cggacactga gccgacggcg
      241 ccgcgcttgc gaaagtgcga agcgcgacgg gcgtcggggg ttgaacaact cgcgttgttc
      301 gcgacgggca gccggcctca gcttcgccag ccgcgattcg cgatgctgat ggcgtggaag
      361 taaatcgcgg cgacgagcgc gatcggcggg gcgtcgtcgg gacggcgaag catttccgcc
      421 atggtggctc gggtggggct gccttcgcgt agcacgaacg tggcgagttg atgagcgagc
      481 gcctggcctt cggcgggagt ggcggtttgc aggctcgcgg gaaactcgaa ggggagcgat
      541 ggcgcgatca tgactggagt gcgggagcga cgtggccgtt ggcggaggac gaggaggagg
      601 ccttcgcgac gaactcggcg atatcaaggt tgagcgcttt cgcgacgccg aggccgtaag
      661 caggatcggc gcggaaaaag tgacagatct ggcggagctg aatctcgcgc ggcacgccgg
      721 ccatgtggcg ggcgatattg ccgaagaggc gttccttttg atcgggcgtg aaaaggcgga
      781 agagatttcc gggctgcgtg tagtcgtcgt tgccgtcgcg gtgattgtag cgatcgacgt
      841 cgccggaaat tttcagcggc ggttcgttga gcgcggggca gtccttcggg ccgttgaagg
      901 agttgggttc gtagttgacg gcgctgccgc cgttgccgtc gaagcgcatg gcgccatcgc
      961 gttgatagtt gaaaatgggg caccgcgggg cgttgaccgg gagatgctgg tgattgacgc
     1021 cgaggcgata gcggtgggcg tcggcgtagg agaagatgcg cgattggagg accttgtcgg
     1081 gcgagtggcc gatgccggga acaacgttgg cgggcgagaa ggcggcctgc tcgacctcgg
     1141 cgtgatagtt ttgcggattg cgattgagct cgaggacgcc gatgtcgatc agcgggtagt
     1201 cggcgtgcgg ccagactttc gtgaggtcga acgggttgac cttgtaggag tcggcgtcgg
     1261 cttcgggcat gatttgcacg cagaagcgcc agcgcgggaa attgccgtcg gcgatcgcgt
     1321 ggaagagatc ggcctgcgag ctctcgcgat tttggccgac gatggcggcg gcctcggcgt
     1381 cggtgtaggt ggcgatgcct tggagggatt tgaagtggaa cttcacccag tagcgttcgc
     1441 cgatgacgtt gatgaaagaa tacgtgtgcg agccgaagcc gtgctgttgg cggaggttct
     1501 tcgggatgcc gcgatcgctc atcaggatcg tgacctggtg aagcgattca ggagagagcg
     1561 accagaaatc ccaagcggcg gtgttgttgc gcaggttggt ttgcgggtcg cgtttttgcg
     1621 tgtggatgaa gtcgggaaa
//
LOCUS       DASPLT010000109         1762 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_144970, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000109 DASPLT010000000
VERSION     DASPLT010000109.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1762)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1762)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1762
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_144970"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(398..1363)
                     /locus_tag="VEA63_01160"
     CDS             complement(398..1363)
                     /locus_tag="VEA63_01160"
                     /inference="COORDINATES: protein motif:HMM:NF033542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HYD82616.1"
                     /translation="MMQLSTPRFNRVIAFEVAKQSLVVHALPEDRQRRIANTAGAVRK
                     LLRTAIESGDGQILVVCEATGGYEKHVLAEACALGLACHRAHGARVRHFAKFMGAAAK
                     TDAIDGRMLALYGQNAQNLRLYRPPAIEQKAVRALKSRRDELLQMMIGETNRIEHADH
                     ASVRASLRTHVRILKLQLARIERELAQIIDETPRLAEQARLMQSVKGVGSETAAAIIA
                     YFPELGSLSKGQAARLAGLAPIARDSGKASAPRHIEPGRAALRRTLYMAAVAAIRFNP
                     GLRAFAAKLKAKGKPFKLIAAAVMRKLVVILNAVLASGEPTRQYA"
BASE COUNT          310 a          551 c          578 g          323 t
ORIGIN      
        1 aaagcagcgc cgtcgcctcc aaccagcaca cgcttcccgg ccgtagcgca gcgaagagcc
       61 gggacccagg ggcaacacac gcaactggtc gctcctggat cccggctcgg cgattcgccc
      121 gcatctgcga tgcgcgcaag cttgtccggg aagcgttgga cgcgcggcgc gcgtcgctcc
      181 acctggcttc tcttacgcgc cccctcacgc acgcttcccg gccgcagcgc agcgagagcc
      241 gggatccagg ggccaacact cagcacgcgg cccctagatc ccggctctgc gcacgcccgc
      301 atcgttgatg cgcgcgtgct tggccgggaa gcgtgctggc acgcactgtc tttgtccggg
      361 aagcgttgaa agcacgctct atctaccgtg ttcgcagtca agcgtactgg cgcgttggtt
      421 cgccgctggc gagcacggcg ttaaggatga cgacgagctt tcgcatcacg gcggcggcga
      481 tgagcttgaa gggtttgcct ttggccttga gcttggcggc gaaggcgcgc aggccaggat
      541 tgaagcggat cgcggcgacg gcggccatat agagcgtgcg ccgcaaggcg gcgcgtcccg
      601 gctcgatgtg acgcggcgct gaagccttgc cgctgtcgcg ggcgatcgga gcgagccccg
      661 cgagccgagc ggcttggcct ttactgagcg agccgagctc tgggaagtag gcgatgatcg
      721 cagcggcggt ttccgatccg acgcctttga cgctttgcat caggcgggcc tgttcggcca
      781 ggcgcggggt ctcgtcgatg atctgagcga gttcgcgctc gatgcgggca agctgcaact
      841 tcaagatccg gacatgggtg cgaaggctgg cgcgcacgct cgcatgatcg gcgtgctcga
      901 tacggttggt ttcgccgatc atcatctgca gcagttcgtc acgccgtgac ttcaatgcgc
      961 gcacggcttt ctgctcgatc gccggcggac gatagagccg cagattttgc gcgttttgcc
     1021 cataaagcgc gagcatgcgc ccatcgatcg cgtcggtctt ggcggcggcg cccatgaatt
     1081 tggcgaagtg acgcacgcgc gcgccgtggg cgcggtggca cgcgaggccg agcgcgcatg
     1141 cctcggcgag cacgtgtttt tcgtagccgc ctgtggcttc gcagacgacc aatatttgcc
     1201 catcgcctga ttcaatcgcc gtgcgcagaa gcttgcgtac ggcgccggcc gtgttggcaa
     1261 tacgccgctg ccgatcctcg ggcagggcgt gcacgacaag agactgcttg gcgacttcga
     1321 aggcgatgac gcggttgaac cgcggcgttg atagctgcat catgagtgaa tctcgggctc
     1381 ctggattgca tgcggccgca gaggcctttc taccgttcgg aggtcctggg atgagcagcg
     1441 ggggtcgcta gatagagggc gaccgctaag gtcaatccaa cacgcgaccg cccgccgccc
     1501 ggagcggggc gttggcccaa cgtcccgctc caacctcacc ttctcgcgat tcttgcagcc
     1561 ccgattgcgg ggataggctc agatacaatc caacactttt ccgcgctaaa gcattgaact
     1621 tgcacacaac aaaagaaatc tatccgcccc ccaaaaagct gagggataat cctcctaccg
     1681 tttagcagga gagcagtcgg ccgctgttcg aaaccgatgc gagaacggtg tgatcgagcg
     1741 tgatgcgtag aggcttctca aa
//
LOCUS       DASPLT010000110         1728 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_83214, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000110 DASPLT010000000
VERSION     DASPLT010000110.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1728)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1728)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1728
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_83214"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1728)
                     /locus_tag="VEA63_01165"
     CDS             complement(<1..>1728)
                     /locus_tag="VEA63_01165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD82617.1"
                     /translation="VKAGAGSPENFLAYADFDNTSAGRKPLHHYTRHLAHYRAGDPTW
                     KNGKGKAIVGALNYLASKKMNSLYFLTMNVAGDGDDVFPWTAKSERYRYDVSKLAQWE
                     IVFEHMDKLGIHLHMVTQEQENDQLLDRGALGVQRKLYYRELVARFSHHLGLTWNLGE
                     ESTNTTAQRESFADYINALDPYLHLIAVHTFPQDHDKVYTPILGHDVIGAASLQVHSP
                     SIVHTETLKWVDKSADTGRKWIVAVDEIGGASVGVKPDANDAEHRVIAHRVLWGSLLA
                     GAAGVEWYFGYDYAHDDLTCEDWTTRENMWTLTHHAAQFVRDYLPLPLVGNYNAITSA
                     TGDYCFGKPGVAYAIYLPAGATTSITLPAGETYTVEWFDPRKGGGLRNGTVATVSGGG
                     AAAIGRPPADTSLDWVALLRRSGSAGGEPEPEKPAPTLAIAELMLVNAKTQADVRPLT
                     DGAIIDLSVDGAVLNVRAAVNGSAGSVAFYLNGKKTLENTAPWTMAGDLNGVFQPWKP
                     AVGRHTLRVVPYSRSNGGGEAGETLEISFTVIAVAPPTTEPPPAPEPENPATLALAEF
                     VLVDAANQR"
BASE COUNT          311 a          600 c          491 g          326 t
ORIGIN      
        1 tcgcgctgat tcgcggcgtc cacgagcacg aattccgcca acgcgagtgt cgccggattc
       61 tccggttccg gtgcaggcgg cggctccgtc gtgggcggcg cgaccgcgat cacggtgaac
      121 gaaatttcca acgtttcgcc cgcttcgccg ccgccgttcg atcgcgaata cggcacgaca
      181 cgcaacgtgt gcctgccgac ggctggcttc caaggctgga agacgccgtt caagtcgcct
      241 gccatcgtcc acggcgccgt gttctccagc gtcttcttgc cattcagata aaacgcgacg
      301 ctgcccgcgc taccgttcac cgcagcgcgc acgttcaaca ccgcgccatc caccgacaga
      361 tcgatgatcg cgccgtccgt taacggacgc acgtccgcct gcgtcttcgc gttgaccaac
      421 atcaactccg caatcgcgag cgtcggcgcc ggcttctccg gctcaggctc cccgccggcc
      481 gaaccgctgc gccgcagcaa agcgacccag tccagcgacg tgtcagccgg cggacggcca
      541 atcgccgcag cgccaccgcc actcaccgtc gcgaccgtgc cattgcgcaa accaccgcct
      601 ttgcgcggat cgaaccattc caccgtgtaa gtctcgcccg ccggcagcgt gatggacgtc
      661 gtggcgcccg ccggcagata aatcgcataa gcgacgcccg gcttgccaaa gcaatagtcg
      721 cccgtcgcgg aggtgatcgc gttgtaattt ccgaccagcg gcaacggcag ataatcgcgc
      781 acgaactgcg ccgcgtggtg cgtcagcgtc cacatgttct cccgtgtcgt ccaatcctcg
      841 caggtcaaat cgtcgtgcgc gtagtcgtat ccaaaatacc actccacgcc agcagcgccg
      901 gcgagcaggc tgccccagag cacacggtgc gcgatcaccc gatgttccgc gtcgttcgcg
      961 tcgggcttca cgcccacgga cgctccgccg atctcgtcga ccgccacgat ccatttgcgc
     1021 ccggtgtccg ccgacttgtc cacccacttc aacgtttctg tatgcacgat cgacgggctg
     1081 tgcacctgca acgaagccgc gccgatcacg tcatgaccca ggatcggagt gtaaaccttg
     1141 tcgtggtcct gaggaaacgt gtgcaccgcg atcaaatgca gatatgggtc gagggcgttg
     1201 atgtagtcgg cgaacgactc ccgctgcgcc gtggtgttcg tcgattcctc gcccagattc
     1261 caggtgagac cgagatgatg gctgaagcgc gccacgagtt cgcgatagta aagtttgcgc
     1321 tgcacgccga gtgcgccgcg gtcgagcagt tgatcgttct cctgctcctg cgtgaccatg
     1381 tgcagatgaa tccccaactt gtccatgtgc tcgaacacga tctcccactg cgccagcttg
     1441 ctcacatcgt aacgataacg ctcggacttc gccgtccacg ggaacacatc gtcgccgtca
     1501 cccgccacgt tcatcgtgag gaagtagagc gagttcattt ttttcgacgc aagatagttg
     1561 agcgcgccga cgatcgcctt gcccttgccg ttcttccacg tcggatcgcc cgcgcgataa
     1621 tgcgcgagat gcctggtgta atggtgcagc ggtttgcggc cggccgacgt gttgtcgaaa
     1681 tccgcatacg ccagaaaatt ctccgggctc cccgcgccgg ccttcacg
//
LOCUS       DASPLT010000111         2181 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_226670, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000111 DASPLT010000000
VERSION     DASPLT010000111.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 2181)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2181)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2181
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_226670"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..482
                     /locus_tag="VEA63_01170"
     CDS             <1..482
                     /locus_tag="VEA63_01170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HYD82618.1"
                     /translation="ASFTLATKTASVPLNMFADAYATYGTVLAENAVVLVQGNIIVGT
                     EGPRINIKEAYPLDAQIAGNVRKVVWLLHPRHRELASFLQQLRSTLDKQSGETRTEFA
                     FVFENRIAPYAEASNALTWKINAPAFQQLRAHPAVAGVQLETRRLELKQDRRWSKRS"
     gene            900..>2181
                     /locus_tag="VEA63_01175"
     CDS             900..>2181
                     /locus_tag="VEA63_01175"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HYD82619.1"
                     /translation="MNSRTTPGPARILVIDDTTSIHEDFRKILRRAAPTASALLDAHA
                     SLFGDKMPPPPDLGFEIDCASQGQEGLALLQGALAENRPYALAFVDVRMPPGWDGVET
                     IRHLWAADPTLQVVICTAYSDHSWEETVRQLGHGDSLLILKKPFDTIEALQLANALTK
                     KWAIARRDRELLADLDERVRQRTAELRSSEERFAKIFHANPVPTTLQHRDDGRYLDAN
                     PAFLQLIGHSRAAVLGRTPAELGLWPASDLWTEALQSIDARTPLRSHRAQLCQRDGTL
                     REVLVSVEQADFATQRCVLTSLEDITDRALLEQQFRQAQKMEAVGQLAAGVAHDFNNL
                     LTVVQAYSGFVLEEATLSEEHRNGLTQVRAAAERAAALTRQLLVFSRRQLAQPEPLDL
                     TITFASLREMHARLLPAEIKVEWNCESDIPRVNAD"
BASE COUNT          415 a          869 c          538 g          359 t
ORIGIN      
        1 gggccagctt cacgctcgcc acgaaaaccg cgtcggtccc gctcaacatg ttcgccgatg
       61 cctatgcgac ctacggcacc gtcctcgccg aaaacgcagt cgttctcgtg caaggcaaca
      121 tcatcgtcgg caccgagggc ccgcgcatca acatcaagga agcctacccg ctcgacgcgc
      181 agatcgccgg caacgtccgc aaggtcgtct ggctccttca cccgcgccat cgcgaactcg
      241 cctccttcct ccagcagctt cgctccacac tcgacaaaca gtccggcgaa acccgcaccg
      301 aattcgcctt tgttttcgaa aaccggatcg cgccctacgc cgaagcaagc aacgcgctca
      361 cttggaagat caacgctccc gctttccaac aactccgcgc ccacccggcc gtcgccggtg
      421 tccagctcga aacccgtcgc ctcgaactca aacaagaccg ccgctggtcc aaacgcagct
      481 gaccgattga tgtgggaggg gccttacgcc tcgactgatc ccgcgcccca accgcccaat
      541 aaatacagtg gcgtggcagc gccggtaggg acgcctctcc gaggcgtccg cgtggccgga
      601 atcgcggtga attaataaat ttatcgctga ggcaccctac tagctggact gcccgcattc
      661 cattttcgcg ctccgtgcgc tctgcgatcc atgcctttct gatttgtcag taatctatcc
      721 ggggaaacac aaaagctcgc gccagcgcct ctctcgaacc gcttcgccgc cccggcgagc
      781 gccgcgcacc catcgctcgc gccgcgcagt tggacaaacg ccgcgtcctg cggcttcttt
      841 ttgtcgttcg agcgataccc gacccacccc ggtcgtgcac cccctgctgt atccgtccca
      901 tgaattcacg cactacccct ggccccgcgc gcattctcgt gatcgacgac accacttcga
      961 ttcatgagga ctttcgcaaa attctccgcc gcgccgcccc gaccgcgtcc gcgctcctcg
     1021 acgcccacgc cagcctgttc ggcgataaaa tgcccccgcc gcccgacctc ggcttcgaga
     1081 tcgattgcgc atcacaaggt caggaaggcc tcgcgctcct ccaaggcgcc ctcgccgaaa
     1141 atcgccccta cgcgctcgcg tttgtcgacg tgcgcatgcc gcccggctgg gacggcgttg
     1201 aaacaatccg ccacctctgg gccgccgatc ctacgctgca agtcgtcatc tgcaccgcct
     1261 actccgatca ctcttgggag gaaaccgttc gccagctcgg ccacggcgac agcctgctca
     1321 tcctcaaaaa acctttcgac acgatcgaag ccctccaact cgccaacgct ctcaccaaaa
     1381 aatgggcgat cgcccgccgc gaccgcgagc tcctcgccga cctcgacgaa cgcgtgcgcc
     1441 agcgcaccgc cgagctccgc agctccgagg aacgcttcgc gaaaatcttc cacgccaatc
     1501 ccgttcccac caccctccag caccgcgacg acggccgtta cctcgacgcc aaccccgcct
     1561 tcctccaact catcggccac tcacgcgccg ccgtcctcgg acgcacgccc gccgaactcg
     1621 gcctctggcc cgccagcgat ctttggaccg aagcgttaca atcgatcgac gcgcgcacgc
     1681 ccctccgctc ccaccgcgcc caactttgcc aacgcgatgg cacactgcgc gaagtcctcg
     1741 tctccgtcga gcaagccgac ttcgcgacgc aacgctgcgt cctcaccagt ctcgaagaca
     1801 tcaccgaccg cgccctgctc gaacaacaat tccgccaggc gcaaaagatg gaggccgtcg
     1861 gccagctcgc cgccggcgtc gcgcacgatt tcaacaacct gctcaccgtc gtgcaggctt
     1921 acagcggctt cgtgctcgag gaagcgacgc tctccgaaga gcatcgcaac ggcctcacgc
     1981 aagtccgcgc cgccgccgaa cgcgccgctg cgctcacccg ccagctcctc gtcttcagcc
     2041 gccgtcagct cgcccagccc gagccgctcg atctcacgat cactttcgca agcctccgcg
     2101 aaatgcacgc ccgcctgctg cccgccgaaa tcaaggttga gtggaattgc gaaagcgaca
     2161 tcccgcgcgt caacgccgac g
//
LOCUS       DASPLT010000112         1630 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_247285, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000112 DASPLT010000000
VERSION     DASPLT010000112.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1630)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1630)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1630
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_247285"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..299)
                     /locus_tag="VEA63_01180"
     CDS             complement(<1..299)
                     /locus_tag="VEA63_01180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD82620.1"
                     /translation="MSITVVGVMSLGMALAFGTRMLVAGQGQMVAGQRAAEAMESVFK
                     ARDSRIVTWDQIRNVVGEGEAPGIFLDGPQEIRDPGDDGLINTEDDGDIEEVITPG"
     gene            complement(344..847)
                     /locus_tag="VEA63_01185"
     CDS             complement(344..847)
                     /locus_tag="VEA63_01185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD82621.1"
                     /translation="MFGISIIAFQAAATTLQGDANLRVLEGQLKYARDVAVSERRSVQ
                     VVLTNTEIGQRKLATIQVIRLDRPAGTTLLSSAVLENNTEFLDFDTVPDTPESFGPSS
                     VYAPGDDAPYMFTADSMFTDEAGSPANATIFIGQKGKVITARAVTVFGTTARIRTYRW
                     NGAEWGH"
     gene            complement(1105..1617)
                     /locus_tag="VEA63_01190"
     CDS             complement(1105..1617)
                     /locus_tag="VEA63_01190"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HYD82622.1"
                     /translation="MAKGLTSAYVQLGAVGLRRPLADFFRDKVFAGGLTYNSHTLACA
                     AAVATLDVYEDDGLIERAAATGKVMKARLEALAAKHPCVGAVRSIGLFGIVELVRNRE
                     TKEPMAPFNGTSPEMKALGAYFREHGLYTFVRWNTFFTNPPLTITEAELDEGFAIIDA
                     ALEAADRAVT"
BASE COUNT          290 a          533 c          504 g          303 t
ORIGIN      
        1 ccgggcgtga tgacttcctc gatgtccccg tcgtcctccg tgttgatcag cccgtcgtcg
       61 ccgggatcgc gaatctcctg ggggccgtcg agaaaaatac ctggcgcctc gccctcccca
      121 acgacattgc gaatctgatc ccacgtgacg atgcgggagt cgcgtgcctt gaagaccgac
      181 tccatcgctt cagcagcgcg ctgtccggcg accatctggc cctgcccggc cacgagcatg
      241 cgcgtgccga acgccagcgc catgcccagc gacatcacac cgacgacagt gatggacatc
      301 gccacgagcg cttcaacgag tgtgaaacct gattccctgc gcatcagtgt ccccattccg
      361 cgccgttcca gcgatatgtg cggatgcgcg cggtcgtgcc gaacacagtc accgcccgcg
      421 cggtgatcac tttgcccttc tgtccaatga agatcgtcgc gttggccgga ctgccggctt
      481 cgtccgtgaa catgctgtcg gccgtgaaca tgtacggcgc gtcgtcaccg ggcgcgtaca
      541 cgctcgaagg gccgaaggat tccggggtgt cgggcaccgt atcgaaatcc aggaactccg
      601 tgttgttctc gagaaccgcg ctcgagagga gcgtggtgcc cgcgggccga tcgagccgga
      661 tgacctgaat cgtcgccagc ttgcgttgcc caatttcggt attcgtcagc acaacctgca
      721 ccgaacgccg ctcactgact gcgacgtcgc gcgcgtattt cagctgtcct tcgaggacgc
      781 gcagattcgc atccccctgt aacgtcgtcg ccgcagcctg gaaggcgatg atcgagatgc
      841 cgaacaacac cccgatgatc gcgacgatcg tgaggatttc aggaagactg aacccacgat
      901 cgtcggcgat cacgcgaggc ctgggagtgg tcatgccgcc agtctgctga gcaagggcca
      961 ttccgcggtc ttatcggccg ctaagatcat tgggaacaga cagttacgga gaaacgtcga
     1021 agacgctgcg aattcggcgt acttacgatt ttcgtccgat tctgccggat ctgtcgctcg
     1081 acgcgcctga tatccgggcg cgtcttacgt aacggccctg tccgccgcct cgagggcagc
     1141 gtcgatgatc gcgaatccct cgtccagctc cgcctcggtg atcgtcaggg gcgggttcgt
     1201 gaagaacgtg ttccagcgga cgaacgtata gagcccgtgc tcgcggaagt acgcgcccag
     1261 cgccttcatc tccggcgacg tgccgttgaa cggcgccatc ggctccttcg tctcgcggtt
     1321 gcgcacgagt tcgacgatgc cgaacagccc gatagagcgc acggcgccga cacatgggtg
     1381 cttcgcggcg agcgcctcga ggcgggcctt catcactttg ccggtcgcag cggcccgttc
     1441 gatcaggcca tcgtcctcat acacgtccag cgtcgcgacc gcggccgcgc acgcgagcgt
     1501 gtggctgttg taagtgaggc cgccggcgaa gactttgtcg cgaaagaaat cggcgagagg
     1561 gcggcgcagg ccgacggcgc cgagctgcac gtacgcgctc gtgagaccct tggccatcgt
     1621 gatcagatcg
//
LOCUS       DASPLT010000113         1394 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_185746, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000113 DASPLT010000000
VERSION     DASPLT010000113.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1394)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1394)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1394
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_185746"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..799)
                     /locus_tag="VEA63_01195"
     CDS             complement(<1..799)
                     /locus_tag="VEA63_01195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase dimerization
                     domain-containing protein"
                     /protein_id="HYD82623.1"
                     /translation="MNSYVAEVLDLVARRNAGEPEFLQAVHEVLESLAPVIERHKKYR
                     DGRILDRVVEPERQIVFRVAWLDDAGRVQVNRGIRVQFNSAIGPYKGGLRFHPSVNLG
                     ILKFLGFEQIFKNALTTLPMGGGKGGSDFDPKGKSDGEVMRFCQSFMTELYRHIGPDT
                     DVPAGDIGVGGREIGYLFGQYKRITNEFTGVFTGKGIKWGGSLIRPEATGYGAVYFAE
                     EMLKTRSEQLAGKVCTVSGSGNVAQFTVEKLLQLGAKPVTLSDSDGSI"
     gene            complement(989..>1394)
                     /locus_tag="VEA63_01200"
     CDS             complement(989..>1394)
                     /locus_tag="VEA63_01200"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HYD82624.1"
                     /translation="IILDESMLRAEQLRELAADAKRWILNVRVSKMGGLVRTLELLGE
                     AKRRGIRVIVGAHVGESSVLTRAALTAASAARDVLVAQEGAFGTHLLARDVVDDSLMF
                     GAGGVLQMEQTGISGASGFGLTIAADLPLRAL"
BASE COUNT          281 a          484 c          357 g          272 t
ORIGIN      
        1 aaatgctgcc gtcggagtcc gacagcgtca ccggcttcgc gcccagctgc agcaatttct
       61 cgaccgtgaa ctgcgcgacg ttgcccgaac ctgacaccgt gcaaaccttc cccgcgagct
      121 gctcgctgcg ggtcttcaac atctcctcgg cgaaatacac cgcgccgtag ccggtcgcct
      181 cgggccggat gagcgagccg ccccacttga tccctttgcc cgtgaacacg ccggtgaatt
      241 cgttggtgat gcgcttgtat tggccaaaca aatacccgat ctcgcgaccg cccacgccga
      301 tgtcacccgc cggcacgtcg gtgtcggggc cgatgtgccg gtaaagctcc gtcatgaagc
      361 tctgacaaaa ccgcatcacc tcgccgtccg attttccctt gggatcgaag tccgaaccgc
      421 ccttgcctcc gcccatcggc agcgtggtca gcgcgttttt gaaaatctgc tcgaatccga
      481 ggaatttcaa aatgcccagg tttacgctcg gatgaaaccg cagcccgccc ttataggggc
      541 cgatcgcgct gttgaactgc acgcgaatcc cgcggttcac ctgcacgcgg ccggcatcgt
      601 cgagccacgc cacgcgaaac acgatttgcc gctccggctc gacgacgcgg tccagaatac
      661 gaccgtcgcg atatttttta tggcgttcga tcaccggagc gagcgactcc agcacttcat
      721 gcacagcttg gaggaattcc ggctcaccgg cattccggcg ggcgacgaga tcgagaactt
      781 cggcgacgta agagttcatt ttatcaatgg gtccccggtg cgatcgatgt cccgcgaggc
      841 tcgcagacat gtttcgaccc ggaacccagg atattcaatc aagccgtgag cacgaacaac
      901 ccggaaattc tctcatcgcg tcttcacttc tccgcttctc acgccaagca gcgtcagtga
      961 tgcgcgcgcc gttgaaaact cggatgcatc agagtgctcg cagcggcaaa tccgcggcga
     1021 tcgttaagcc aaagcccgaa gcgcccgaaa ttccagtttg ctccatctgc aacacgccgc
     1081 ccgctccaaa catcagcgaa tcatcaacca cgtccctcgc cagcaagtgt gtcccaaacg
     1141 cgccctcttg tgcgactaac acatctcgcg cggcactcgc ggccgtcagc gcggctcgcg
     1201 tcagcacgct cgactcaccg acgtgagcac cgacgatgac ccgtattcca cgtcgtttgg
     1261 cttctcccaa gagttccaat gtcctcacca gcccgcccat tttcgaaacg cgcacgttga
     1321 ggatccaccg ctttgcatcc gcggccagct cccgcaactg ctccgcccgc agcatacttt
     1381 catccaggat gatt
//
LOCUS       DASPLT010000114         1027 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_42524, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000114 DASPLT010000000
VERSION     DASPLT010000114.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1027)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1027)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1027
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_42524"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..198)
                     /locus_tag="VEA63_01205"
     CDS             complement(<1..198)
                     /locus_tag="VEA63_01205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD82625.1"
                     /translation="MSFANLTIGKKITLGFALLFALLIVVATIAFTALGGAGRRLALF
                     AGSAQETYVAATLESSMQGLKL"
     gene            complement(225..578)
                     /locus_tag="VEA63_01210"
     CDS             complement(225..578)
                     /locus_tag="VEA63_01210"
                     /inference="COORDINATES: protein motif:HMM:NF015506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HYD82626.1"
                     /translation="MRTLKHYLAIVAVCLAPATPVAFAAAGREAPVPVRTVAPEYPRE
                     LKDSGVSGIVTVKVTIDEQGNVSEADVVKSSNEGFDRFATAALKKWKFKPGKQDGSPI
                     AMQVTIPVKFVLDES"
BASE COUNT          198 a          289 c          343 g          197 t
ORIGIN      
        1 cagcttgagg ccttgcatcg acgactcgag cgtcgcggca acgtaagtct cttgcgcgct
       61 gcccgcgaaa agggcgagcc ggcggcccgc gccgcccagg gcggtgaaag cgatggtggc
      121 gacgacgatc agcagcgcga acaagagcgc gaagccgagc gtgatctttt ttccgatggt
      181 caggttggcg aaggacatga tgaagaattt agagggaacg gagatcagct ctcatcgagg
      241 acgaatttga ccgggatggt gacctgcatc gcgatcggac tgccgtcttg cttgcccggc
      301 ttgaacttcc actttttgag cgcggcggtg gcgaagcggt cgaaaccttc gttgctggat
      361 ttcacgacgt cggcctcgga aacgttgcct tgttcgtcga tcgtgacctt cacggtgacg
      421 atgccggaca cgccggaatc cttgagctcc cgcggatatt ccggcgcgac agtgcggacc
      481 ggaaccgggg cttcacggcc cgcggcggca aaagccacgg gggtggcggg agcgagacaa
      541 acggcgacga tggcgaggta gtgcttgagg gttctcatgt gctaaatgtg gtttcgattg
      601 ttacaaattc gtcacgaacg agcgttcact taaatgtcga tattcgcggc gcaggtctgc
      661 atcgggtgca gggtcaatcg tcacggcttg gcgaaactgt agcggcgagc catcgcgaaa
      721 atgatgattg gctggtgcaa acggcttgag cccttgtccg caggcgaagc ggaggacgcc
      781 gggagcgccg cgcgaaaaga actgcgcggc tgaggcgcgc cgccattgca atcgctgccg
      841 caatccgcac gctcggccgc tcccttcatc cgaccggaat gctgctcgaa gaaaaactcc
      901 gtcgcgcccc caaagcggcc ggctttgtgg tcatcgcgat ctccatgata cgctttggcg
      961 gggcattttt cggagccgag ggcgaaccga tgtccgtgct ctggtccgcg ggtccactgg
     1021 cgctgct
//
LOCUS       DASPLT010000115         1030 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_22795, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000115 DASPLT010000000
VERSION     DASPLT010000115.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1030)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1030)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1030
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_22795"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..210
                     /locus_tag="VEA63_01215"
     CDS             <1..210
                     /locus_tag="VEA63_01215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     GatCAB subunit B"
                     /protein_id="HYD82627.1"
                     /translation="GLKAAPTDAGELEQWCRDSIAANPKAVAEFKAGKDSAINALKGP
                     VMKAGKGKANPKLVDETLRKLLAEM"
     gene            284..>1030
                     /locus_tag="VEA63_01220"
     CDS             284..>1030
                     /locus_tag="VEA63_01220"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RecQ family ATP-dependent DNA helicase"
                     /protein_id="HYD82628.1"
                     /translation="MNQDPVAAARALLKERFGFDRFRPGQEEVIAHLLAGRSALAVFP
                     TGSGKSLCYQLPALLLDGLTVVVSPLIALMKDQTDFLQARGIAAARLDSSVSREAFEE
                     IQNALREGRLKLLYVAPERFSNERFLAKLKTLATDLLVIDEAHCISEWGHNFRPDYLK
                     LARYARELRVGRVLTLTATATPAVAKDICREFAIEPAAHVHTGFYRPNLHLRATECGE
                     GRRLEALIARLKAQPAGPTIVYVTLQKTAET"
BASE COUNT          191 a          293 c          352 g          194 t
ORIGIN      
        1 ggcttgaagg cggcgccaac cgatgcaggc gagctcgagc agtggtgtcg cgactcgatc
       61 gccgctaatc cgaaagcagt cgccgaattc aaggccggca aggacagcgc gatcaacgcg
      121 ctcaaaggcc ccgtaatgaa agccggcaaa ggcaaagcca atccgaagct cgtggacgag
      181 acgctgcgca agctcctcgc ggagatgtga cgtcggcggc gaacgcgctt ctgtcgaatc
      241 tttgcgcttg gcgtttggga cccgccgcca agtttgtgcg gcgatgaatc aggatccggt
      301 cgctgccgcg cgtgcgttgt tgaaggaacg tttcggtttc gatcgctttc ggccgggaca
      361 ggaggaggtg atcgcgcatc tgcttgcggg gcgttcggcg ctggcggtgt ttccaacggg
      421 cagcggcaag agtctgtgtt atcaactccc ggcgttgttg ctcgacgggc tcacggtcgt
      481 cgtctcgccg ctgatcgcgc tgatgaagga tcagacggat tttctgcagg cgcgcggaat
      541 cgcggcggcg cgactcgatt ccagcgtgag tcgcgaggcg ttcgaggaaa ttcaaaacgc
      601 gctgcgcgag ggccggctga agctgttgta tgtggcaccg gagcgttttt cgaacgagcg
      661 ctttctcgcg aagttgaaaa cgctggcgac cgacctgctc gtgatcgacg aggcgcactg
      721 catttccgag tgggggcaca actttcggcc ggactacctg aagcttgcgc gctatgcgcg
      781 cgagttgcgg gtcggtcgcg tgctgacgtt gaccgccacc gccacgccgg cggtggccaa
      841 ggacatttgt cgcgaattcg cgatcgagcc cgcggcgcat gtgcacacgg gtttctatcg
      901 gccgaacctt catttgcgcg caacggaatg cggcgagggt cggcgactcg aagcgttgat
      961 cgcgcgactg aaagcgcagc ccgccggacc gacgatcgtg tatgtcacgt tgcaaaaaac
     1021 cgcggaaacg
//
LOCUS       DASPLT010000116         1021 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_1884, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000116 DASPLT010000000
VERSION     DASPLT010000116.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1021)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1021)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1021
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1884"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..470
                     /locus_tag="VEA63_01225"
     CDS             <1..470
                     /locus_tag="VEA63_01225"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HYD82629.1"
                     /translation="HTHAMPYELRHGTYLISDDPALLDADAIHAYLTRSYWAAGIPRD
                     VVVRSITHSLCLGIYANERQQIGLVRVISDYATFAYLCDVYVLEPHRGRGLSKAAMRL
                     LTSHPRLQNLRRQQLVTDSAHSLYAQFGFKPLAHPERHMEKRNPNVYRDAPIS"
     gene            complement(612..>1021)
                     /locus_tag="VEA63_01230"
     CDS             complement(612..>1021)
                     /locus_tag="VEA63_01230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="iron ABC transporter substrate-binding protein"
                     /protein_id="HYD82630.1"
                     /translation="LPVRRDYYEQEEWNAYRSDPEVDPFEETERLVYRPAWTGGVFRE
                     MAFIIRVMTLDTHAELERAWRAINAAEDARRERALAVLQDMSAVDYELAKTDIKRALS
                     SKNKVDEITLGRELGSRFRAQYARAERVARGEE"
BASE COUNT          190 a          399 c          236 g          196 t
ORIGIN      
        1 gccacactca tgccatgcct tacgagctcc gtcacggcac ctatctgatc agcgacgatc
       61 ccgcgttgct cgatgccgac gcgattcacg cttacctcac ccgcagctac tgggccgcag
      121 gcatcccgcg cgacgtcgtc gttcgctcga tcacccactc gctctgcctc ggcatctatg
      181 ccaacgaacg tcaacaaatc ggcctcgttc gcgtgatctc cgactacgcg accttcgcgt
      241 atctctgcga cgtctacgtg ctcgagccgc atcgcggtcg cggcctctcg aaagccgcga
      301 tgcgcctgct cacctcgcac ccgcgcctgc aaaatctccg tcgccagcaa ctcgtcaccg
      361 atagcgccca cagcctctac gcccagttcg gcttcaagcc cctcgcgcac cccgaacgcc
      421 acatggagaa acgcaacccg aacgtctacc gcgacgcgcc catctcataa tcgttctcgt
      481 tcttcgttct ctttctcgtt ctcgaactcc gcgacgtccg agaaaaaccc cgagagaacg
      541 agaaggatta agagaacgag aacgatttaa gttctgagaa cggtcgccac gcatcatgcc
      601 cgaccccgcc gctactcctc accccgcgcg acccgctccg cccgcgcata ctgcgcgcga
      661 aaccgactcc cgagttcccg tccgagcgtg atctcgtcga ccttgttctt cgacgacagc
      721 gcccgcttga tgtccgtctt cgccagctcg tagtccaccg ccgacatatc ctgcagcacc
      781 gccagcgccc gctcgcgccg cgcgtcctcc gcggcgttga tcgcgcgcca cgcccgttcg
      841 agttcggcat gtgtatccaa agtcatcaca cgaatgatga acgccatctc gcgaaacacc
      901 cctcctgtcc aggccggccg atacaccaac cgctccgtct cttcgaaagg gtcgacttcc
      961 ggatcgctcc gatacgcgtt ccattcctct tgctcgtaat agtcgcgcct caccggcaat
     1021 c
//
LOCUS       DASPLT010000117         1143 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_83270, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000117 DASPLT010000000
VERSION     DASPLT010000117.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1143)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1143)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1143
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_83270"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1143
                     /locus_tag="VEA63_01235"
     CDS             <1..>1143
                     /locus_tag="VEA63_01235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HYD82631.1"
                     /translation="GVFARTIQVALGYAWFLADEEVDTDQTRIEANYRFELGKTEHNL
                     LLGRTDLTRVRRRTSSGTNNLPTQQYNWRAPDDFSHFRYDPANQVPLNKQSDEDLHRN
                     DAGHYLVYQGKYFSDRLNIIGGIRHDRADGKRVYRDLVTGETGSVQGSKSGKPTTKWS
                     PQYGASFALTREITIFGLGSSGLSPNDDKTDGAGYVMRPTTAKSLEAGFKIDLFNGRV
                     SGTISAYHIERNDVPQYFWWAPAPVRPGYDASLPITYRPFWDARNRLYYVDDPVDRGI
                     LQGIFDSMNNPNQLAPADNYLYAGADVNQPRSGAYAPVNDESKGADAQIILTLKPNWQ
                     VVLGYSHVERKLVQGPELVAAPYSPFSPWFALSNSPVGIWGGGPVG"
BASE COUNT          231 a          344 c          343 g          225 t
ORIGIN      
        1 tcggcgtgtt cgcccgcacg attcaagtgg cgctcggtta cgcgtggttt ctcgccgacg
       61 aggaggtcga caccgatcaa acccgcatcg aagcgaacta ccggttcgag ttgggaaaga
      121 ccgaacacaa tctgcttctg gggcgcacgg atcttacgcg cgtgcgccgg cgcacgtcct
      181 ccggcacgaa caatctcccg acgcagcaat acaattggcg agcgccggac gatttctctc
      241 atttccgcta tgatccggct aatcaggtcc cgctcaacaa gcagtcagac gaggatctgc
      301 atcgcaacga cgccggtcac tatctcgtat atcaaggtaa atacttttcc gatcgcctca
      361 atatcatcgg cggcatccgc cacgatcgcg ccgacgggaa acgggtctat cgcgatctcg
      421 tgaccggcga gaccggaagc gtgcagggga gcaagtcggg caaacccacc accaagtgga
      481 gcccgcaata cggcgcgagt ttcgccctga cgcgtgaaat tacgatcttc ggtttgggtt
      541 cgtccggtct ttcgccgaat gacgacaaga cggacggcgc cggctatgtc atgcgtccca
      601 cgacggcgaa gagcctggaa gccggtttca agattgacct cttcaatggt cgcgtgtcgg
      661 gcacgatcag cgcctatcac atcgagcgga atgacgtgcc ccaatatttc tggtgggcgc
      721 ccgcgcctgt gcgtccggga tatgatgcga gtttgccgat cacctaccgt ccgttctggg
      781 acgcgcgcaa ccgtctctac tacgtcgatg acccggtcga tcggggaatc ctgcaaggga
      841 tcttcgactc gatgaacaat cccaaccagc tcgcgccagc ggacaactat ctatatgccg
      901 gcgccgatgt gaatcagcca aggagcggcg cctatgcgcc ggtgaatgat gagtcgaagg
      961 gcgcggatgc ccagatcatc ctcacgttga aacccaactg gcaggtagtg ttggggtatt
     1021 cgcacgtcga gcgcaaactc gtgcagggcc ctgagctggt tgccgcgccg tattcgcctt
     1081 tcagtccttg gttcgcgttg agcaactcgc cggtgggcat ctggggcggc gggccggtgg
     1141 gtg
//
LOCUS       DASPLT010000118         1357 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_185786, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000118 DASPLT010000000
VERSION     DASPLT010000118.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1357)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1357)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1357
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_185786"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..220
                     /locus_tag="VEA63_01240"
     CDS             <1..220
                     /locus_tag="VEA63_01240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD82632.1"
                     /translation="VPVLAETSVMIAAGVGVPIRRIFWATLAPNIAISAIYSLAANDS
                     FATASWVFLATVAVSYLAWRIGLGLRSR"
     gene            526..>1357
                     /locus_tag="VEA63_01245"
     CDS             526..>1357
                     /locus_tag="VEA63_01245"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HYD82633.1"
                     /translation="MNTQADTFAGPASLSANAADVSRGFPPQAEMARRMREYDWSRTP
                     LGPMETWPEELRIALGASPDVAKNTEPQGIPSHPEQENRGLKLLDEAGMRLLTHERPE
                     VLIEELCNGLAELLGFEVFINYLVLDDSRRLRLHASRGIGAEERSLIDVLDFGEAVCG
                     AVADRQRPIIVDDVHGSSDPALGLVRNLGIAAYACHPLVADGELLGTLSFGRRRNGHF
                     RPEELHLMGTLSDQIATALHLHRLRKKWTADVQARRDAEAAAARAEKRFHAIANLVPD
                     L"
BASE COUNT          235 a          380 c          473 g          269 t
ORIGIN      
        1 cgtgccggtg ttggcagaga cgtcggtgat gattgcggcg ggcgtgggcg tgccgatccg
       61 gcggattttt tgggcaacgc tcgcgccgaa tatcgcgatt tcggcgatct attcgctggc
      121 ggcaaatgat tcgttcgcaa ccgcgtcgtg ggtgttcctc gccacggttg cggtatcgta
      181 tctcgcttgg cggatcggac tcgggctccg gtccagataa ccgagccaac cctttgacgg
      241 ttatcctcgg gtttggctca gactgtgtgc cgcagccgcg aggtatggcc gtccaaacag
      301 gtttggctcg ggaaatttcc cgagtcgcgc ccggtgattg tccggcgaga tgggcgggac
      361 ctcacacgtg caatcgggcg acaaatcggt ggaactgtcc gcgggctgtt ctgaccatct
      421 tcgttcttcg tccttgtctg gaccgccgaa tcaggccgcg acgggttggt ctcgggttgg
      481 aaggtgcaag gcgcaaggaa ggatcttcga gcggctgccc agaacgtgaa cacccaagct
      541 gatactttcg ccggtccggc gtcgttgagc gcgaacgctg cggacgtgag tcgcgggttt
      601 ccaccgcagg ctgagatggc ccggcggatg cgggaatacg actggtcgcg aacgccgctc
      661 gggccgatgg agacctggcc ggaggaactg cggattgcgc tgggcgcatc gccggacgtt
      721 gcgaagaaca ctgagccaca gggaatacca tctcacccag aacaggagaa ccgcggactg
      781 aagctgttgg atgaggccgg catgaggctt ctcacgcatg agcggcccga ggtgttgatc
      841 gaggagctgt gcaatggact ggctgagctg ctcgggtttg aggtgttcat caactacctc
      901 gtgctcgatg acagtcgccg gctgcgtttg cacgcgagcc gagggattgg agcggaagag
      961 cgctcgttga tcgacgtgct ggatttcggc gaggcggtgt gcggcgcggt cgcggatcgg
     1021 cagcggccga tcatcgtcga tgacgtgcat ggatcgagcg accccgcact tgggttggtg
     1081 cgaaatctcg ggatcgcagc ttatgcctgc cacccgctcg tcgcagacgg tgaattgctc
     1141 ggcacacttt cgttcggccg gcggcgcaat ggtcatttcc ggccggagga gctgcacctc
     1201 atgggcacgc tgtcggatca gatcgcgacg gcgctacacc tgcaccggct ccgaaaaaaa
     1261 tggacagctg acgtccaagc gcggcgtgac gcggaagccg ccgccgcgcg ggcggagaaa
     1321 cgttttcacg cgatcgcgaa tctggtgccg gatcttt
//
LOCUS       DASPLT010000119         1702 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_226800, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000119 DASPLT010000000
VERSION     DASPLT010000119.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1702)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1702)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1702
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_226800"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..811
                     /locus_tag="VEA63_01250"
     CDS             <1..811
                     /locus_tag="VEA63_01250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD82634.1"
                     /translation="SIRMGLPPVVDPGPGTNPPEEPGTNPETPGNGDPVGEGNPGGPN
                     DPGQVVIITLPADERTRAFIAELREILRTRSGDPQQFVLEFLNRMNPAPTSSVNPMLA
                     DSSVSSGRIDSNSVLSESTEPVASFGDGGNGAALASAYVGYQFGVDQPLVSGTSKIFG
                     NKLPDGMFAVPAIDIDVFWPVAPLAAPSSTSARPLDESVEEAPSEQPTLEESLHAFGS
                     YLVERVSAEFFPGQQSLVLLVDPKPSRSSVPTRNSALDEFANGGRRIASVV"
     gene            944..1465
                     /locus_tag="VEA63_01255"
     CDS             944..1465
                     /locus_tag="VEA63_01255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyltransferase/cytidyltransferase family
                     protein"
                     /protein_id="HYD82635.1"
                     /translation="MTAPLKPVLPLPKLRTLDNARREREQLAAAGQKVVLTNGCFDLL
                     HTGHLYFLQEAAKLGDALFVALNGDASVRALKGPHRPIQSAHERAYALAALACVHTVV
                     VFDEPRLVREIEALRPDIYAKAGDYSLATLDSSERDALHAVGAEVRFLPFLPNFSTTA
                     LLKRIAAAHAFAE"
     gene            1477..>1702
                     /locus_tag="VEA63_01260"
     CDS             1477..>1702
                     /locus_tag="VEA63_01260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD82636.1"
                     /translation="MDVSPARIQEICAAFPHARIVVIGDVMLDHLVVGDARRISPEAP
                     IPVVNYRHETHSPGGAANVARNLAALGCHAD"
BASE COUNT          292 a          650 c          445 g          315 t
ORIGIN      
        1 ttctattcgc atgggattgc cgcccgtggt cgatcccggc cccggcacga acccgcccga
       61 agagcccggc acgaatccgg agacacccgg caacggcgat ccggtcggcg aaggcaaccc
      121 cggcggcccg aacgatcccg gccaagttgt catcatcacc ctgcccgccg acgaacgcac
      181 ccgcgccttc atcgccgaac tacgcgagat tcttcggacg cgctccggcg acccacagca
      241 attcgtcctc gagtttctga accgcatgaa tccggcgccg acgagttcag tgaacccgat
      301 gctcgccgat agctctgtca gctccggacg catcgattcc aactccgttc tctcggagtc
      361 gacagagccg gtagcatcat tcggcgatgg cggcaacggc gcggccctcg cgtcggccta
      421 cgtcggttat cagttcggcg tcgaccaacc tctcgtttcg ggaaccagca agattttcgg
      481 caataagctg cccgacggca tgtttgccgt gcccgccatc gacatcgacg ttttctggcc
      541 ggtcgcgccg ctcgctgccc ccagcagcac ttccgcgcgc cccttggacg aaagcgtcga
      601 agaggccccc tccgagcagc ccactctcga agaaagcctt cacgcgtttg gcagctacct
      661 cgtcgagcgt gtctccgccg agttttttcc tggacagcag tccttggttt tactcgtcga
      721 cccgaaaccc tcgcgctcat cggtccccac ccgcaactct gccctcgatg agttcgccaa
      781 cggtggccgc cggatcgcga gcgtggttta accactcgcc tagcggttag ggagcgcgct
      841 cgcgcccgct caaccagcca ggtggctcgc tgtgtgagca gaggaagatg ccccttggga
      901 aaggatcgca aacccgcgtt ctattctcat cgtgcggcag cgcatgacag ctcccctcaa
      961 gcctgtcctt cctttaccaa agctgcgcac gctggacaat gcgcgccgcg aacgggaaca
     1021 actcgccgcc gccggccaaa aagtcgttct caccaacggc tgcttcgatc ttctccacac
     1081 cggtcatctt tatttcctgc aggaagccgc gaaactcggc gacgcgctct tcgtcgcgct
     1141 caacggcgac gccagcgtgc gcgcgctcaa aggcccgcac cgccccatcc agtccgctca
     1201 cgaacgcgct tacgccctcg ccgccctcgc gtgcgtccac accgtcgtcg ttttcgacga
     1261 accacgcctc gttcgcgaaa ttgaagcgct ccgtcccgac atctacgcca aagccggcga
     1321 ctactcgctc gccacgctag attcctccga gcgcgacgcc ctgcatgccg tcggcgccga
     1381 agttcgcttt ctcccctttc tcccgaattt cagcaccacc gcgctgctga aacgcattgc
     1441 cgccgcccac gccttcgccg agtgaagttt ccgctcatgg acgtctcccc cgctcgtatc
     1501 caggaaatct gcgccgcctt ccctcacgca cgtatcgtcg tcatcggcga cgtcatgctc
     1561 gatcacctcg tcgtcggcga cgcgcgtcgc atttcgcccg aggcgcccat tccggtcgtc
     1621 aactatcgcc acgaaacgca ctcgcccggc ggcgccgcca atgtcgctcg caacctcgcc
     1681 gccctcggct gccacgccga ct
//
LOCUS       DASPLT010000120         2174 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_42560, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000120 DASPLT010000000
VERSION     DASPLT010000120.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 2174)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2174)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2174
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_42560"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..753)
                     /locus_tag="VEA63_01265"
     CDS             complement(<1..753)
                     /locus_tag="VEA63_01265"
                     /EC_number="4.1.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007360170.1"
                     /note="catalyzes the phosphorylation and decarboxylation
                     of oxaloacetate to form phosphoenolpyruvate using GTP;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase"
                     /protein_id="HYD82637.1"
                     /translation="MRNSSKPLSSNKHLLKWVEKIAELTKPSAIHWVDGSQEEYDALW
                     AQMVAAGTAIKLNEKLWPGCYYVRSDPGDVARVEDRTFICSLSKDNAGPTNNWENPFA
                     MRKKLKGLFRGCMRGRTMYVLPFSMGPIGSPMAQIGVEITDSPYVVVNMRIMARIGLP
                     VFAEIDKAEKRVVPCVHSVGAPLANGAEDVPWPCNAEKYIVHFPETREIWSYGSGYGG
                     NALLGKKCYSLRIASAMAHDEGWLAEHMLILKL"
     gene            876..1040
                     /locus_tag="VEA63_01270"
     CDS             876..1040
                     /locus_tag="VEA63_01270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD82638.1"
                     /translation="MSKLCIFAGTTIVGYAGWFAGDALGLGFFGAFLLSGAGSLLGVW
                     LGWKLARKLE"
     gene            complement(1129..2043)
                     /locus_tag="VEA63_01275"
     CDS             complement(1129..2043)
                     /locus_tag="VEA63_01275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HYD82639.1"
                     /translation="MSSELPQHHAATQQRARDSAGLVLRGILLNALLAVLKFTGGIFG
                     NTYALIADGVESLLDILSSVLVWAGFRVAARPPDANHPYGHGKAEPLAALSVAVFIFA
                     AAIWIGWHAIYEIITPHRGPHWATLPLLAGIVATKIWFSRRLHAAGEVAGSTALGAEA
                     LHHYSDAVTSGAAFVGIAIAVIGGEGYEAADDWAALVACAVITFNGVALFSRALEDVM
                     DTAVSDEFENEVRARALAVVGVSALDKCRVRKSGLSHLVDIHVCVNGNLTVREGHDIA
                     HAVKDALLESPLRVSDVSVHIEPTPLTR"
BASE COUNT          422 a          801 c          580 g          371 t
ORIGIN      
        1 cagcttgagg atcagcatgt gctcggcgag ccagccctcg tcgtgcgcca tcgccgaggc
       61 gatccgcagc gagtaacact tcttgccaag cagggcgttg ccgccgtaac ccgagccgta
      121 gctccagatc tcgcgcgtct ccgggaagtg cacgatatac ttttccgcat tgcacggcca
      181 cggcacatcc tccgcgccat tcgcgagcgg cgcgccgacc gaatgcacac acggcaccac
      241 ccgcttctcg gctttgtcga tctcggcaaa caccggcaac ccgatgcgcg ccatgatgcg
      301 catattcacc acgacataag gcgagtccgt gatctcgacg ccgatctgcg ccatcggcga
      361 cccgatcggt cccatgctga acggcagcac atacatcgtg cgcccgcgca tgcacccgcg
      421 gaaaagtccc ttcaactttt tccgcatcgc gaatggattt tcccaattgt tcgtcggccc
      481 cgcgttatcc ttcgacaacg aacagatgaa cgttcggtcc tcgacgcgcg ccacgtcacc
      541 cggatcggaa cgcacgtaat aacaccccgg ccacagcttc tcgttcaact tgatcgccgt
      601 ccctgccgcg accatctgcg cccacaacgc gtcgtattcc tcctgcgatc cgtcgaccca
      661 gtgaatcgcc gacggcttcg tgagctccgc gatcttctcg acccatttca gcaaatgctt
      721 gttcgaggac aatggcttcg atgaattgcg catgagccgg gaagattgag gatggaacat
      781 gctgatagca aactcccgcc aggcttcccg tctgcgcgca tcgtgtaatg ctacgagcaa
      841 aatccataaa cccgcgccct cactctcgac cgcccatgag caaactgtgc atcttcgccg
      901 gaaccacgat cgtcggctac gccggctggt tcgccggcga cgccctcggc ctcggcttct
      961 tcggcgcctt tctcctcagc ggcgccggca gcctcctcgg cgtctggctc ggctggaaac
     1021 tcgcccgcaa actcgagtaa gcccaatcac gtttgtcata taatagatga caaacacgcc
     1081 gcgcccgccg cgctctcggt ttcagccaat gtcatacaat ggatgacatc accgcgtgag
     1141 cggcgtcggc tcgatgtgca cggagacatc gctcacgcgc aacggcgact cgagcagcgc
     1201 gtccttcacc gcatgcgcga tgtcgtgccc ctcgcgcacg gtgagattgc cgttcacgca
     1261 cacgtggatg tcgaccagat ggctgagccc gcttttccgc acgcgacatt tgtcgagcgc
     1321 gctcacgccc accaccgcca gcgcccgcgc gcgcacttcg ttctcgaatt cgtcggacac
     1381 cgccgtgtcc atcacatcct ccagcgcgcg actgaacaac gccacgccgt tgaacgtgat
     1441 caccgcgcac gccacgagcg ccgcccaatc gtccgccgcc tcgtagcctt cgccgccgat
     1501 caccgcgatc gcgatcccca caaacgccgc gcccgacgtg accgcatccg aatagtgatg
     1561 caacgcctcc gcgcccaacg ccgtgctgcc cgccacttcg ccggccgcat gcaaccgccg
     1621 cgaaaaccag atcttcgtcg cgacgattcc cgccagcagc ggcagcgttg cccaatgtgg
     1681 tccgcggtga ggcgtgatga tctcgtagat cgcgtgccag ccgatccaga tcgccgccgc
     1741 aaaaataaac accgccaccg acaatgccgc cagcggctcc gccttgccgt ggccataagg
     1801 atggttcgca tccggcggac gcgccgccac gcgaaaaccc gcccacacga gcacggacga
     1861 aaggatgtcg agcaacgact cgacgccatc cgcgatgagc gcgtaggtgt tgccaaaaat
     1921 accgccggta aacttcagca ccgccagcag cgcattcagc aggatgcccc gcaacaccag
     1981 gcccgcacta tcgcgcgcgc gttgctgcgt cgccgcgtga tgctggggca gttcggaact
     2041 cacgcgcgga aactagacgc acgccccacc cgcgcaaacc cgctttcgct cgtccgtctt
     2101 gcagtagccg ccggcctccg cgcctgcccg ctcgatcgca ggtaacgcac gcagagggtg
     2161 agactcaccg gcgg
//
LOCUS       DASPLT010000121         1038 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_83284, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000121 DASPLT010000000
VERSION     DASPLT010000121.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1038)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1038)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1038
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_83284"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..416
                     /locus_tag="VEA63_01280"
     CDS             <1..416
                     /locus_tag="VEA63_01280"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HYD82640.1"
                     /translation="EAAPERVFDAWLNREMLGQWMWGPNVRDEEIVHLAVDARVGAGF
                     SFMVERDGEEIDHVGKYLELERPRRLVFTWMVVGEAVGSRVLVDIVPEGAGCVLTLTH
                     ELHPDWADFVERSEAAWATMLEMLGEALEGAGEAE"
     gene            complement(597..851)
                     /locus_tag="VEA63_01285"
     CDS             complement(597..851)
                     /locus_tag="VEA63_01285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD82641.1"
                     /translation="MDLDLLQWPAMVTSVTAAWLVAAQSKTRRQHGFWWFLASNILWI
                     AWGWHAHAWALIVLQIALAILNIRGLRKNEPAEPQPEQSR"
BASE COUNT          195 a          241 c          406 g          196 t
ORIGIN      
        1 tcgaggcggc gccggagcgg gtgttcgatg cgtggttgaa tcgcgagatg ttggggcagt
       61 ggatgtgggg accgaatgtg cgcgacgagg agatcgtgca cctggcggtc gacgcgcggg
      121 tgggcgccgg gttctcgttt atggtcgagc gcgacggaga agaaatcgac cacgtgggaa
      181 aatacctcga actggagcgg ccgcggcggc tggtatttac gtggatggtg gtgggcgaag
      241 cggtgggctc gcgcgtgctg gtcgacatcg tgccggaggg cgcggggtgc gtgttgacgc
      301 tgacgcatga gctgcatccg gactgggcgg actttgtgga gcgatcggaa gcggcttggg
      361 cgacgatgct ggaaatgctc ggcgaagcgc tggagggcgc gggcgaggcg gagtgatccc
      421 aacaccgtcg aatttttggc cgcaaagagg cgcagatggc gcaagaagag gaaagtcctt
      481 gaggtctttt ttttgcgcat tctgtgcctc tttgcggcga taattttagt ttatctgctc
      541 tgaattatac gtggcagcgt gatgactgaa ctcagccggc tcgccttcag ttgagttcag
      601 cgggactgct caggctgggg ctcggccggt tcgtttttgc gcaggccgcg aatgttgaga
      661 atcgccaagg cgatctgcag gacgatcaac gcccaggcgt gcgcgtgcca gccccacgcg
      721 atccacagga tgttactggc gaggaaccac cagaagccgt gctgacgtcg ggtttttgat
      781 tgggcggcga cgagccaggc agcggtgaca ctggtgacca tggctggcca ttgaagaaga
      841 tcgaggtcca tggaaagagc ggtggaagaa cgtgtgcagt tgcggactcg tgcggcggag
      901 gaaggttcga gggcgcgcgg cgtcggtggg cgatgcgcga acgatattgc cgaacggcgc
      961 cagcgagcgg aggcaaaggg aagcaatgcg cgattcggcc cgcggtcgga gggattgggt
     1021 tgagaggaaa cgcgagcg
//
LOCUS       DASPLT010000122         1788 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_226839, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000122 DASPLT010000000
VERSION     DASPLT010000122.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1788)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1788)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1788
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_226839"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..751
                     /locus_tag="VEA63_01290"
     CDS             <1..751
                     /locus_tag="VEA63_01290"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HYD82642.1"
                     /translation="IKFTPSRGEVQVTTMVKNDTVVLRVRDTGTGINPEFLPHVFERF
                     RQADASTTRQYGGLGLGLSIVKQLVELHGGEIRAESAGIGRGATFTVSIPRLESSDAA
                     ETSSSPALHQTAPSPSPAELRGVRVLVVEDETDSAQLMKRVLESHQADVRLAQSMPSA
                     LTEFAQFKPHLVLSDIGMPHHDGYELIRRLRALPEARRLPAIAITALARREDIDRALQ
                     AGFDLHLAKPLRPAELLRAVLTLVGRNVTDA"
     gene            909..1286
                     /locus_tag="VEA63_01295"
     CDS             909..1286
                     /locus_tag="VEA63_01295"
                     /inference="COORDINATES: protein motif:HMM:NF019491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dabb family protein"
                     /protein_id="HYD82643.1"
                     /translation="MKKLMLSLLASGLLLLGTTVTAHAANDTAPNTLIHVVTVSWKAG
                     TTPDQVQAALDGVKALPTKFPGIKRVWTKSVKVQGGKANAIVMEFENEAALKEYAGSD
                     AQKEWYKAYTPIRESSTTFDITN"
     gene            complement(1384..>1788)
                     /locus_tag="VEA63_01300"
     CDS             complement(1384..>1788)
                     /locus_tag="VEA63_01300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD82644.1"
                     /translation="TSGREALHEAFAEGLAKAINDGGGAPIEVKTKRVNPDHAAFNLG
                     TGVYDAVLVIANQLPRPLILSETKRLVATLSTGREEKKAFLIFQQADEGLEKVLTAAF
                     TPAITNSRFLDALDGGFEKVDGANGGSKIAAR"
BASE COUNT          342 a          621 c          485 g          340 t
ORIGIN      
        1 catcaagttc accccctctc ggggcgaggt gcaggtcacg accatggtca agaacgacac
       61 cgttgttctg cgcgtgcggg acaccggcac cggcatcaac ccggaatttc tgccgcatgt
      121 ctttgagcgc ttccgccagg ctgacgcttc tacgacgcgg caatacggcg gactcggctt
      181 gggactctcg attgtgaagc agctcgtgga attgcatggc ggcgaaattc gcgccgagag
      241 cgccggcatt ggtcgtggag cgacgttcac cgtcagcatt ccacgcctcg agtcgtccga
      301 cgccgcggag acctcgtcct cacccgcact ccatcagacg gccccttcgc catcgcccgc
      361 cgaactccgc ggcgtccgcg ttctcgtggt ggaggacgaa accgactctg cgcaactgat
      421 gaaacgcgtc cttgagagtc accaagccga tgtccgtctc gcgcaatcga tgccgtcagc
      481 tctcaccgaa ttcgcgcagt tcaagccgca tctagtgctc agcgacatcg gcatgcctca
      541 ccacgacggc tatgaattga ttcgccgtct ccgcgccttg cccgaagcgc ggcgtttgcc
      601 agccatcgcc atcaccgcgc tcgcccggcg cgaagatatc gaccgcgcgc tccaggccgg
      661 gtttgacctg catctcgcca agccgctgcg accggcggaa ctcctccgcg ctgtcctgac
      721 gctcgtcggc cgaaacgtca ccgacgcctg acttccacca gccgatcatt cgcgactggc
      781 gtccggacgc atccaaacca cgctgcatgc gtcaattcct cgcggtcggc ggaaacttcc
      841 gtgaccactg gaagtcatta acaccccggc ttcatgccgg acccaacccc aatcccatcc
      901 aaaagatcat gaagaaactg atgctgtctc tcctcgcctc gggcctcttg ttgctcggca
      961 ccaccgtcac cgcgcacgct gcgaacgaca ccgctcccaa caccctcatc cacgtcgtca
     1021 ccgtttcctg gaaggccggc accacgccgg atcaagtgca ggctgcgctg gacggggtga
     1081 aagcactgcc gaccaaattt cccggcatca aacgcgtctg gaccaagtcc gtgaaggttc
     1141 agggcggcaa agccaacgcg atcgtgatgg aattcgaaaa tgaagctgcg ctgaaagaat
     1201 acgccggcag cgacgctcaa aaggagtggt ataaagctta cacgccgatc cgcgaatcga
     1261 gcacgacgtt cgatatcacc aactgagcgc agatccgctc cccctccccg gccgcctcat
     1321 cgcaggcggc ctttttgttg gactgtcccg agcgggcgag gcggccggtt ggactgcgcc
     1381 ggcctagcgt gcggcgattt tcgatccgcc gtttgcgcca tccacttttt caaatccccc
     1441 gtcgagcgca tcgagaaatc tcgagttcgt gatcgccggc gtaaatgccg ccgtgagcac
     1501 tttttcgagg ccttcgtcag cctgctgaaa aatcaggaac gccttcttct cctcccgacc
     1561 cgtgcttagc gttgcgacga ggcgcttcgt ctcgctgaga atcagcggcc ggggcagttg
     1621 gttcgcgatg acgagcaccg catcataaac acccgtgccg agattgaacg ccgcgtgatc
     1681 cggattcacc cgcttcgtct tcacctcgat cggcgctcca ccgccgtcgt tgatggcttt
     1741 ggccaaacct tcggcaaacg cctcgtgtaa cgcttcacgg ccactcgt
//
LOCUS       DASPLT010000123         1652 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_62988, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000123 DASPLT010000000
VERSION     DASPLT010000123.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1652)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1652)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1652
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_62988"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1014)
                     /locus_tag="VEA63_01305"
     CDS             complement(<1..1014)
                     /locus_tag="VEA63_01305"
                     /inference="COORDINATES: protein motif:HMM:NF012876.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HYD82645.1"
                     /translation="MLRTRLYLGLLPLLLLLVGMGAYAIRVSRELAGSLQRDLVGNYR
                     DILACQQLRTSARLMTSALSIADRGDPFEARRNFDENRAAFTRELMAQSATSAGTSRA
                     PLVQQLDDAFDELTRLSEALLGSGGSGSLEMLQRNETALYRVLAAIESLNQRDFAAAQ
                     QTALRAERMVETTERVLYTAMLGALVLSLLVAWRLAASLLRPIKALTTSAVAVGEGDL
                     DRVVPVLSRDELGRLAQSFNQMAAKLRAYREATLAKVLRTQRTMEATLTSAPDPVFVV
                     ARDGTQEVRNPAAEELARSVGFAEGFPPALSEPLAAVLTSGEHYLPTDYGRVVTLRVG
                     REDR"
     gene            complement(1034..>1652)
                     /locus_tag="VEA63_01310"
     CDS             complement(1034..>1652)
                     /locus_tag="VEA63_01310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HYD82646.1"
                     /translation="DEIGELPLEIQPKLLRLLQEREYERVGEARVRRANVRLIAATNR
                     NLAEEVRAGRFREDLFYRLNVITIALPGLHDRPADLLRFADNYRQFFAARLGKKIAPF
                     SPAVVSALTAYKWPGNLRELRNVIERAAILAPGNAIELADLPEELGAAGGPAIEVGAH
                     VSIDALEAEHIRRVLRTTRNLDEAARTLGIDPATLYRKRQKLGLV"
BASE COUNT          305 a          615 c          464 g          268 t
ORIGIN      
        1 ccggtcctcg cgaccgacgc gcaacgtcac cacgcgcccg taatccgttg gcaaataatg
       61 ttcgccgctc gtcaataccg ccgcgagcgg ctcggacagc gccggcggaa atccctccgc
      121 aaatccgacc gaccgcgcca actcctctgc cgccggattc cgcacttctt gggttccgtc
      181 gcgcgccacc acgaacaccg gatcgggcgc cgacgtgagc gtcgcttcca tcgtgcgttg
      241 cgtgcgcagc actttcgcca atgtcgcctc acgatacgcg cgcagcttcg ccgccatctg
      301 attgaacgac tgtgcgagcc gcccgagctc gtcgcgcgac aacaccggca ccacccgatc
      361 caaatcgcct tcgcccaccg ccaccgccga cgtcgtcaat gccttgattg gtctcaacaa
      421 cgacgccgcc aatcgccacg ccaccaacaa cgacaacacc agcgcgccga gcatcgccgt
      481 gtagagcacg cgctcggtcg tctccaccat tcgctccgcg cgcaacgccg tctgttgcgc
      541 cgccgcaaag tcgcgctggt tcaaactctc gatcgccgcg agcacgcgat agagggccgt
      601 ctcatttcgc tgcagcatct cgagcgatcc cgagccgccg ctgccgagca gcgcttcgct
      661 cagccgcgtg agctcgtcga aggcgtcgtc gagctgttgc acgagcggcg cacgcgacgt
      721 tcccgccgac gtcgccgact gcgccatcaa ctcgcgcgtg aacgccgcgc gattctcgtc
      781 gaaatttcgc cgcgcttcaa acggatcgcc gcgatccgca atcgacagcg ccgacgtcat
      841 cagccgtgcc gacgtgcgca attgctggca cgcaaggatg tcgcggtaat tgccgacgag
      901 atcacgttgc agcgagccgg cgagttcacg cgacacgcga atcgcgtagg ctcccattcc
      961 caccagcaac aacaacagcg gcagcaggcc gagataaagt cgcgtgcgaa gcatggcggg
     1021 agcgcgtggc gcgctaaacg agaccgagct tctggcgttt ccgataaagc gtcgccggat
     1081 cgatgccgag cgtgcgcgcc gcttcgtcga gattccgcgt cgtgcgtaac acgcggcgga
     1141 tgtgctccgc ctcgagcgca tcgatcgaca cgtgcgcgcc gacttcgatc gcaggaccgc
     1201 cggccgcgcc cagttcctcc ggcagatcgg ccaactcgat cgcattcccc ggcgcgagga
     1261 tcgccgcgcg ttcgatcacg ttgcgcaatt ctcgcaggtt acccggccac ttgtaagcgg
     1321 tgagcgcact caccaccgcg ggcgaaaacg gcgcgatttt ctttccgagc cgcgccgcga
     1381 aaaactgccg gtaattatcc gcaaagcgca gcaaatccgc cggccggtcg tgcaagcccg
     1441 gcaacgcgat cgtgatcacg ttcaaccgat aaaacagatc ttcgcgaaag cgccccgccc
     1501 gcacttcctc cgccagattg cggttcgtcg ccgcaatcaa ccgcacgttc gcgcgccgca
     1561 cccgcgcctc gcccacgcgc tcgtattcgc gttcctgcaa caaccgcagc agtttcggct
     1621 gaatctcgag cggcagttcc ccgatctcgt cc
//
LOCUS       DASPLT010000124         2682 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_103531, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000124 DASPLT010000000
VERSION     DASPLT010000124.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 2682)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2682)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2682
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_103531"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..240)
                     /locus_tag="VEA63_01315"
     CDS             complement(<1..240)
                     /locus_tag="VEA63_01315"
                     /inference="COORDINATES: protein motif:HMM:NF013255.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HYD82647.1"
                     /translation="MKIAVVGAGGFVGTRLIEHFHLAKDGPVSVTAVVRRPEGLALPA
                     RFAIETYIADPLDVDTMARAFSGCTAVIHCSMGDIT"
     gene            complement(237..1328)
                     /locus_tag="VEA63_01320"
     CDS             complement(237..1328)
                     /locus_tag="VEA63_01320"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HYD82648.1"
                     /translation="MPSSSPRRIILVGCGAIARQLYVPALRALQDAGVARVSAIVDPA
                     VTAREAIAQTFPRAGQSAALEQTTAPANSLVILTSPTRFRAAQAMVALEKGWHVFCEN
                     PVGSTVAETADAIAAAQRHQRLLAVNLYQRFFPACRYLRSLCRDWLLGPLVSYTINEG
                     GSFRASDDAANFDRAQEPGGVLFEAGPNVFDLLGWCLGEPSNIRYADDAMGGLEANAF
                     VQLDYANGPRGRVHLSRDCLPAQEYRFVFERGVVTWRGNDAGHLAVELAGAPAVLRGS
                     LLSLHGENLSSADPLETVPQCFLLQLQNVLAAIAGEETLLAPAEEAQSSLRLIEQCYA
                     HRALVDQPWLTPDEAARAHELGSAVATTS"
     gene            complement(1354..2511)
                     /locus_tag="VEA63_01325"
     CDS             complement(1354..2511)
                     /locus_tag="VEA63_01325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD82649.1"
                     /translation="MPSLLYIGENPGAGTGSPIIILRHLQRFAADGWMISVIGDYGGD
                     YRACHAAGWSVTQLCHRRWWWPPYRQKFASLRWLRLRLLAYEAAAANPSPDAILSYFA
                     AHSDFSAQLATHVAQVTGAPLHMLAHDDAASFPYARGRELELRRFHESILSAADACWF
                     VSPELADCYPATAPRRRILYPIPEGWHQPAVWNEERVRQPRVYYAGYVWPQQLPLLAR
                     EARAAHAAGAELVIMSRESVALRALCDTEPVRWHAPFPTNQEALAHLVADAAGLIVSY
                     ADTIAAMPWCETSFPSKLVEYCHLGLPIAIVAPNDSAIARWAQRVRFPYFFEPSNMKA
                     LQDWFSGLRQREVWQVRSALSLHLARTEFDPRRIHSSLADALIPGTERRAA"
BASE COUNT          530 a          789 c          970 g          393 t
ORIGIN      
        1 cgtaatgtcg cccatcgaac aatgaatcac tgcggtgcaa ccgctgaaag cgcgcgccat
       61 cgtatcgacg tcgagcgggt ccgcgatgta agtctcgatt gcgaaccgcg ccggcagcgc
      121 aaggccttcg ggccggcgca cgacggcggt gacggaaacg ggtccatctt ttgcgagatg
      181 gaaatgctcg atcaaacgcg tgccgacgaa accgccggcg ccaacaacgg cgattttcat
      241 gaggtcgtgg cgacggccga gccgagttcg tgggcgcgcg cggcttcgtc gggcgtgagc
      301 cacggctgat ccacgagcgc gcggtgcgcg tagcattgct caatgagccg aagcgaagat
      361 tgcgcttctt cggcgggagc gagcagcgtt tcttcgccgg cgatggcggc gaggacgttt
      421 tggagttgga ggagaaaaca ctgtggaacg gtttccagcg ggtcggcgga ggagaggttt
      481 tcgccgtgaa gcgagagcag tgaaccgcgc agcacggccg gcgcgccggc gagctccaca
      541 gcgagatgac cggcgtcgtt gccacgccac gtgacaacgc cgcgttcgaa aacgaaacga
      601 tattcctgcg cgggcagaca gtcgcggctg agatgcacgc ggccgcgggg accgttggca
      661 taatcgagtt gcacgaaggc gttggcctcg agcccgccca tcgcgtcgtc ggcgtaacga
      721 atgttcgagg gttcaccgag gcaccagccg agcagatcga agacattcgg gccggcctcg
      781 aagagcacgc cgccgggctc ctgcgcgcgg tcgaaattcg cggcgtcgtc ggacgcgcga
      841 aacgagccgc cctcgttgat cgtgtagctc acgagcggac cgagcagcca gtcgcggcag
      901 aggctccgca gataacggca cgcggggaaa aagcgctgat agagattcac ggcgagcagc
      961 cgttgatggc gttgcgcggc ggcgatcgcg tcggcggttt cggcgacggt ggagccgacg
     1021 ggattctcgc aaaacacgtg ccagcctttc tcgagcgcga ccatcgcctg cgcggcgcga
     1081 aaccgcgtgg gcgacgtcag gatgacgagc gagtttgccg gcgcggtggt ttgttccagt
     1141 gccgccgact ggccggcgcg ggggaaggtc tgcgcaatcg cctcgcgtgc ggtcaccgcc
     1201 ggatcgacga tcgcggagac gcgcgcaacg ccggcgtctt gcagcgcgcg caatgccggc
     1261 acgtaaagct ggcgcgcgat ggcgccgcag ccgacgagga tgatgcgacg aggtgaagat
     1321 gaaggcatga gcagcggaaa acgggagact ttgtcacgcc gcgcgacgtt cagtgcccgg
     1381 aatcaaggcg tccgcgagcg atgagtgaat gcgcctggga tcgaactcgg tccgcgcgag
     1441 atgcagcgac agtgcggagc gcacctgcca gacttcgcgc tggcgaagac cggagaacca
     1501 atcctgcagc gctttcatgt tggacggttc gaagaagtag gggaaacgca cgcgctgcgc
     1561 ccaacgcgcg atcgcactgt cgttgggcgc gacgatcgcg atcggcaaac cgaggtggca
     1621 gtattcgacg agtttgctcg ggaagctcgt ctcgcaccaa ggcatcgccg cgatcgtatc
     1681 ggcataggag acgatcaggc ctgcggcgtc ggcaacgaga tgcgcgagcg cctcctgatt
     1741 cgtggggaag ggcgcgtgcc agcgaaccgg ctcggtgtcg cacaaggcgc gcaacgcgac
     1801 cgactcgcgt gacatgatga caagttcggc cccggcggcg tgcgcggcgc gcgcttcgcg
     1861 agcgagcagt ggaagctgct gcggccagac gtagcccgcg tagtaaacgc gaggctggcg
     1921 aacgcgttcc tcgttccaca ccgcgggctg atgccagcct tcgggaatcg gatagaggat
     1981 gcgacgacga ggcgcggtgg cgggataaca atccgcgagc tcgggcgaca caaaccagca
     2041 ggcgtccgcg gcgctgagaa tgctttcgtg aaaacgacgc agctcgagtt cgcgtccgcg
     2101 cgcgtaggga aaagacgcag catcgtcgtg cgcgagcatg tgcagcggcg caccggtgac
     2161 ctgcgccacg tgcgtggcga gctgtgcgga aaaatcggaa tgcgccgcga agtagctgag
     2221 aatcgcgtcg ggcgaggggt tcgccgccgc cgcttcgtaa gcgaggagcc gcaagcgcag
     2281 ccagcggagg ctggcgaact tctggcggta gggcggccac caccagcggc ggtggcagag
     2341 ctgggtgaca gaccagccgg cggcgtgaca cgcgcgataa tcgccgccgt agtcgccgat
     2401 cacagagatc atccaaccgt cggctgcgaa tcgctggaga tgacgtaaaa tgatgatcgg
     2461 acttccggtt ccggcgccgg gattctcgcc gatgtagaga agagaaggca tggtatgagc
     2521 ttggaaaaaa aggggaacgt gcggcggctc agcgcagcat tgcttcgacg ggcgaagcgg
     2581 aggacggcga cgcctcggcc cagtcgagaa acgcgcccac atcgcgactc gcgacatagg
     2641 gttgcgcggc ttgcaagcgg gcgtcgggcc acgtccacca tt
//
LOCUS       DASPLT010000125         1128 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_42624, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000125 DASPLT010000000
VERSION     DASPLT010000125.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1128)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1128)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1128
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_42624"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..383
                     /locus_tag="VEA63_01330"
     CDS             <1..383
                     /locus_tag="VEA63_01330"
                     /inference="COORDINATES: protein motif:HMM:NF016497.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HYD82650.1"
                     /translation="TLGEPKRLFHGSDAPWSQKGSQRGDHVTDGPWLYRTQGGKLLML
                     WSSFSSTGYAVGIATSVSGKLAGPWEQSPTALFTADGGHPMLFRRFDGQLMMALHQPN
                     KPPHEREQFIELEEINDTLVLKRR"
     gene            631..>1128
                     /locus_tag="VEA63_01335"
     CDS             631..>1128
                     /locus_tag="VEA63_01335"
                     /inference="COORDINATES: protein motif:HMM:NF019556.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-L-arabinofuranosidase domain-containing
                     protein"
                     /protein_id="HYD82651.1"
                     /translation="MKSLPLFLCALATGLTQPAVSAATADSPWRIADKAPARVELFDL
                     ADVRLAPGPFLDAQQRTAAYLLSLSADRLLHGFRKNAGLPPKAPHYGGWEARGLAGHS
                     LGHYLSACAMMYSSTGDERFRERVAYIVAELAACQTPARGGLISGFPDDARVFAEVAA
                     GDLRVQ"
BASE COUNT          191 a          429 c          294 g          214 t
ORIGIN      
        1 cgacgctcgg cgaaccgaag cgcctcttcc atggcagcga tgcgccgtgg agccagaagg
       61 gctcgcaacg cggcgaccac gtgaccgacg gcccctggct ttatcgcacg cagggcggca
      121 agctgctgat gctttggtcg agcttttcct ccaccggcta cgccgtgggc atcgccacgt
      181 ccgtgtctgg aaaactcgcc ggcccctggg agcaatctcc caccgcgctc ttcaccgctg
      241 acggcggtca cccgatgctc ttccgccgct tcgacgggca gctgatgatg gctttgcacc
      301 agccgaacaa accgccgcac gaacgcgaac agttcatcga actcgaagaa atcaacgaca
      361 ccctcgtgtt gaaacgccgc tgacagccga ctgcatcgca ccatcgcacg ccggcttctc
      421 agccccaact accctggcgc atccgttcaa cgagccaaac ggtgagcaca gaggaacaca
      481 tgcagctccg agctttcctc gtcgtccgca ttcgtccgcc tgaaaaactt ccaaacactt
      541 tgttgcttcc tgcttcctct gtgtcggcgc tccgtgcgct ctgtgcaaca agccctattt
      601 gatttgggtg tgatctattc gcgctaccgc atgaaatccc ttcctttatt cctctgcgct
      661 ctcgccaccg gcctcacgca acccgctgtc tccgccgcaa ccgccgactc tccgtggcga
      721 atcgccgaca aagctcccgc acgcgtcgag ctcttcgatc tcgccgacgt ccgtctcgcg
      781 cccggtccgt ttctcgacgc ccagcagcgc accgccgctt accttctcag cctctccgcc
      841 gaccgcctcc tccacggctt ccgcaaaaac gccgggcttc cgcccaaagc accgcactac
      901 ggcggctggg aagcccgcgg tctcgccggt cactcgctcg gccactatct ttccgcctgc
      961 gcgatgatgt attcgtcaac gggagacgaa cgcttccgcg agcgcgtcgc ctacatcgtc
     1021 gccgagctcg ccgcctgcca aacgccggcg cgcggcggat tgatcagcgg ctttcccgat
     1081 gacgctcgcg tgttcgccga agtcgccgcc ggcgatctcc gcgtgcag
//
LOCUS       DASPLT010000126         1755 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_103534, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000126 DASPLT010000000
VERSION     DASPLT010000126.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1755)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1755)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1755
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_103534"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1299
                     /locus_tag="VEA63_01340"
     CDS             <1..1299
                     /locus_tag="VEA63_01340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD82652.1"
                     /translation="ERRDTKSYVAALQGSWWESKDGSYYHLSGLYGWREDTQRNHSQS
                     FPRNALGEYLVPVTPDRAYHLIEPNGVWGTPSEITAHTTSYNVMLRPISQVRFFYNYS
                     DIFRAAAATFADISGTSLRPAYGETEDYGVKVDLFDERVFISATKYETAQRDSSVDNT
                     GTIREPIQQILAGVVQNAGLPANQQVGPAASTAELQEITRLQDDLIRPFAYRDDSTEG
                     YEFAVTASITSNWNARLTFGIQKTTVSALAEEWLPYHEQYLPFWQKFAANGLVNQTDA
                     NYMNVGHAIARAQQRLVDYRSIIGQQPGDQRTRNSSLATSYRFTEGRLKGFRIGGGYR
                     WASENIIGYARDASGNLDRERPFRGKAEFATDASIGYTRRFGSGGKFNWDVQLNVYNL
                     LDDQSIRAYQAVDDGNGNPLIARTYLPEPLTVQLTNTISF"
     gene            1465..>1755
                     /locus_tag="VEA63_01345"
     CDS             1465..>1755
                     /locus_tag="VEA63_01345"
                     /inference="COORDINATES: protein motif:HMM:NF033679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNRLRE domain-containing protein"
                     /protein_id="HYD82653.1"
                     /translation="MIPQKTLQPLLASLVTLTLWSVAPVQAVTLNPTADAYVRSGVNQ
                     PINYGNATTLEIKKSANPADDFNREIYLKFDLSGVTSIRTAKLRLYGSAGSPE"
BASE COUNT          359 a          593 c          488 g          315 t
ORIGIN      
        1 gagcgccggg atacaaagag ctacgtcgcc gccctccaag gttcctggtg ggaatcgaaa
       61 gacggcagtt actatcatct ctcgggcctc tatggatggc gtgaagacac ccagcgcaac
      121 cattcgcaga gcttcccgcg caacgcgctt ggcgaatacc tcgtcccggt cacgccagac
      181 cgcgcgtatc atctcatcga accgaacggc gtctggggca caccgagcga gatcacggcg
      241 cacacgacga gctataacgt catgctccgg ccgatttcgc aggttcggtt cttctacaac
      301 tactccgata ttttccgcgc ggcggcagcg acgttcgccg acatctccgg gacgtcgctt
      361 cgtccggcct atggcgaaac cgaggactac ggcgtcaaag tggatttgtt cgacgagcgc
      421 gtcttcatct cggcgacgaa atacgaaacc gcccagcgag actcttctgt cgacaacacg
      481 ggaaccattc gcgaaccgat tcagcagatc ctcgccggcg tcgttcaaaa cgcaggcttg
      541 cccgcgaatc aacaggtcgg acccgccgca tcgaccgcgg agctccagga aatcacgcga
      601 ttgcaggacg acctgatccg gccgttcgct taccgcgacg atagcacgga gggctatgag
      661 ttcgcggtca cggcgagcat cacgtcaaat tggaacgcac ggctcacctt cggcattcag
      721 aagacaacgg tgtcggcgct ggccgaggaa tggctgcctt accacgaaca gtatctgccg
      781 ttctggcaga agttcgcggc caacggcctg gtcaaccaga ccgatgcgaa ttacatgaac
      841 gtcggccacg cgatcgctcg cgcccagcaa cgcctcgtcg attatcgctc gatcatcggc
      901 cagcaacccg gcgatcaacg cacgcgcaac tcgagtctcg ccacgtccta tcgtttcacc
      961 gaaggccggt tgaaaggctt ccggatcggc ggcggttatc gctgggcctc ggaaaacatc
     1021 atagggtatg cgcgcgatgc ttcggggaat ctcgatcgcg agcgtccgtt ccgcggcaaa
     1081 gcggaattcg cgaccgacgc cagcatcggt tacacccgtc ggttcggctc gggcggcaag
     1141 ttcaactggg acgtgcagct caacgtctac aacctgctcg atgatcaaag cattcgcgcc
     1201 taccaggccg tcgatgacgg caatggcaat ccgctcatcg cgcggaccta cctgccggag
     1261 ccgctcacgg tccagttgac caacacgatc agcttctaag cgcgggcgtc cgctcgacgc
     1321 tctcggcggc gcggcttcgg tcgcgtgcgc tttccctcaa tcgtttctct cccgccgggc
     1381 gcgcctcatg ggtgcgtccg gcgcggcggc gccttgccgc tgcgattcgc tccagctcac
     1441 cacctcttag ctgaagacga tcccatgata ccccaaaaaa cactgcagcc gctcctcgcg
     1501 agcctcgtta cgctgactct ttggtccgtc gccccagtgc aggccgtgac gctcaacccc
     1561 accgctgacg cctacgtgcg atcgggcgta aatcagccta tcaactacgg caacgccacc
     1621 acgctcgaga tcaagaaaag cgcgaatccc gcggacgatt ttaaccgcga gatctatctc
     1681 aaattcgacc tgagcggcgt caccagcatc cgcaccgcca agctgagact ctacggctcc
     1741 gccggctcgc cggag
//
LOCUS       DASPLT010000127         2107 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_42633, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000127 DASPLT010000000
VERSION     DASPLT010000127.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 2107)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2107)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2107
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_42633"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..407)
                     /locus_tag="VEA63_01350"
     CDS             complement(<1..407)
                     /locus_tag="VEA63_01350"
                     /inference="COORDINATES: protein motif:HMM:NF018682.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four-carbon acid sugar kinase family protein"
                     /protein_id="HYD82654.1"
                     /translation="MDAAAAFHSGGANVKALLEPMSLTRFRDDAVVAVTTETRNAAPV
                     LAAAAVENALTIARACGRELWFKKIDSTLRGPVAAELSALAAAMPRTRILFAPANPAA
                     GRTVQQGILRVHGVPVAETEFGRDPANPVRTSSL"
     gene            complement(464..1489)
                     /gene="pdxA"
                     /locus_tag="VEA63_01355"
     CDS             complement(464..1489)
                     /gene="pdxA"
                     /locus_tag="VEA63_01355"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: protein motif:HMM:TIGR00557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HYD82655.1"
                     /translation="MPDLAKPVLAVTLGDASGTGPELITKAFCEPSVRSLCRPVVVGD
                     ASTLRAAVAITGVGSIVRVITNLRDTADDAAAIDVFDLNNVDVAHLVRGGISAAAGRA
                     AYESIHQAVALTRAGDAEAIVTSAINKAALHQAGFHYDGHTELLAELCGNPPVTMMLV
                     ADKLRVCHVSTHVSLHEAISRVRAERIIEVVQLAREGLRNLGIAEPRIAVAGLNPHAG
                     EGGLFGEEEIKVIAPAIAEARRRGAHVSGPYAGDTVFFRTLQGEFDCAIAMYHDQGHV
                     AAKMLGIWQGVNVTLGLPIIRTSVEHGTDFQNAGKGTGDPRSLVQALKLAATMALTRR
                     TIAGRTS"
     gene            1844..1975
                     /locus_tag="VEA63_01360"
     CDS             1844..1975
                     /locus_tag="VEA63_01360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD82656.1"
                     /translation="MDYIAAYNRQSQPFVWAASADLILGKVATYVANWSDRTLIILR"
BASE COUNT          443 a          652 c          617 g          395 t
ORIGIN      
        1 agtgagctgg tgcgaaccgg gttcgcggga tcgcgaccaa attcggtttc agcaacaggc
       61 acgccgtgca cgcgcagtat tccttgttgg accgtgcgac cggcggccgg gttagcgggc
      121 gcaaagagaa tacgcgttcg cggcatggca gccgccagcg cagagagttc cgcagctacg
      181 ggcccgcgaa gcgttgagtc gatttttttg aaccacagct cacggccgca cgctcgggcg
      241 atcgtgagcg cattttcaac tgcagcggca gccaacaccg gcgcggcatt tcgcgtttcg
      301 gtggttacgg cgacgacggc atcatcacga aatcgcgtga gcgacatggg ttcgagcaag
      361 gccttaacgt tggcgccgcc agaatggaat gccgccgcag cgtccaacaa gccgctcaga
      421 tcgtcggcga gaatcagccc gctcatgaca gaaacggtgc cgcctaactc gtccgtccgg
      481 cgatcgtgcg gcgagtgagc gccatcgttg cagcgagctt gagcgcttgg acgaggctgc
      541 gcggatcgcc ggtgcctttg cccgcgttct gaaaatcggt gccgtgctcc acggatgtgc
      601 ggatgattgg tagaccgagc gtgacgttca cgccttgcca gatgcccagc attttggccg
      661 cgacatggcc ttgatcgtga tacatcgcga tggcgcagtc gaactcacct tgaagggtgc
      721 ggaaaaaaac cgtatcgccc gcatacgggc cggagacatg cgccccgcgt cgccgcgcct
      781 cagcgatggc gggtgcgatg acctttatct cttcctcgcc gaacaagccg ccttcgccag
      841 catgagggtt aagacccgcg acagcgatgc gtggctcggc gatacccaag ttacgtaaac
      901 cttcgcgcgc caattggacc acttcgatta ttcgctccgc gcgaacgcgg ctgatggcct
      961 cgtgaagaga aacgtgggtg ctgacgtggc agacacgcaa tttatccgcc acgagcatca
     1021 tggtcacagg cgggtttccg cacaactcgg cgagaagttc cgtgtggccg tcgtagtgaa
     1081 acccagcttg gtgcagcgca gccttgttga tggcggaagt gacaatggcc tcggcgtcgc
     1141 cagcgcgcgt caaagcgaca gcttggtgga tagactcgta agccgcccgg ccggccgcgg
     1201 cggatatccc gccgcgaacg agatgagcta catcaacatt gttaagatcg aacacatcga
     1261 tcgcagccgc gtcatccgct gtgtctcgca aattggtgat cacccggaca atcgagccta
     1321 cgcccgttat ggcaacagca gcgcggagag tagacgcgtc gcccacgacc acaggacgac
     1381 acagagatcg aacgctcggc tcgcaaaacg ccttggtgat cagctccgga ccagttccgg
     1441 acgcgtcgcc caaagtcacc gccaatacag gctttgccaa gtcaggcata tcgctcgcgt
     1501 tgtaacgtta cccgaaaacg cagcaacgta tttaaacggt taataaatac tatagcataa
     1561 ggatcggcgt attgagccgc gcgcgccaat tcctggtttt cctgaagcga atcgacgcgg
     1621 ccatccccgc cacctcaccc tgcatctggt gctcgacaac tacgcgaccc acaagacccc
     1681 cgccgtgcgt cgatggtggt gacgccactc gcgcgtcatc tccactttat tccaatccac
     1741 agctcctggc tcaatcaagt ggaacgatgg ttcgccaaaa tcacgacccg aagccatccg
     1801 ccgtggcagc ttccgctccg tcccgcatct gcgccagacc atcatggact acatcgccgc
     1861 ctataaccgc cagtcccagc cttttgtctg ggcggcctcc gccgacctca tcctcggcaa
     1921 agtcgccact tatgtggcga actggtctga cagaacacta ataatacttc gttaagtaag
     1981 tggtcaccct tggcgaagat tttccgcacg ctgcgggcat gaccgagaag caactgtcgg
     2041 gggtgcacaa gcggctcgat cggtttttgc gtgagctggt cgagccgatg ggacgaagcg
     2101 agcgtcg
//
LOCUS       DASPLT010000128         1406 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_267748, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000128 DASPLT010000000
VERSION     DASPLT010000128.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1406)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1406)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1406
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_267748"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..922
                     /locus_tag="VEA63_01365"
     CDS             <1..922
                     /locus_tag="VEA63_01365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007363203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HYD82657.1"
                     /translation="IATLNPKGDPVLMLAGHIDELGLIITYVNKDGFLYFDTVGGHDL
                     SVISGRRVIIQTADGPVKGVTGKRAIHLMDEEDRKKVPKKHEIWIDIGARSKKDALER
                     VAIGDVATYDHEFELFNGSIGAARAFDDKVGAYVVGETLIRLAREKKKLAAKIVSVAT
                     AQEEIGVRGATTAVYTVNPHIAVAVDVGHATDHPDCDNRKYGETKLGGGPIICRGANI
                     NPKIYQRLVAAAKKANVPCQLEADPRPTGTDARAIQMGRGGVATGLVSIPLRYMHTPS
                     EVVDLEDVERCVKLLVEFAKALAPGDYAHW"
     gene            complement(927..>1406)
                     /locus_tag="VEA63_01370"
     CDS             complement(927..>1406)
                     /locus_tag="VEA63_01370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HYD82658.1"
                     /translation="REKIRAALRTYRAKVDALEKETPYGLPYKPDIWGAGWGIQRFGV
                     EQYFLHAGAPDIFPRDYMLHAINFVLGCHPGSNNASFVSGVGAKSLTTAYGVNRGDWS
                     YIPGGVASGTALIRPDYPELLDWPFLWQQTEYCLGRPTSDYVFLILAADHWLNDRGA"
BASE COUNT          299 a          440 c          436 g          231 t
ORIGIN      
        1 catcgccacg ctgaacccga aaggcgaccc ggtgctcatg ctcgcgggcc acatcgacga
       61 gctcgggctc atcatcacct acgtcaacaa agacggcttt ctctatttcg acacggttgg
      121 cgggcatgat ctcagcgtga tctccggtcg ccgtgtcatc atccaaaccg ccgacgggcc
      181 agtgaagggc gtcaccggca aacgcgcgat tcacctcatg gacgaagagg atcgcaagaa
      241 ggtgccgaag aagcacgaga tctggatcga tatcggggcg cgctcgaaaa aagatgccct
      301 cgaacgcgtc gcgatcggtg acgtcgcgac ctacgatcac gagttcgagt tgttcaacgg
      361 cagcatcggc gcggcccggg cgttcgacga caaagtcggc gcctacgtgg tcggcgaaac
      421 actcatccgg ctcgcgagag aaaagaagaa gctcgccgca aaaatcgtgt ctgttgcgac
      481 ggcgcaggag gaaatcggcg tccgcggtgc cacgacggcg gtctacaccg tcaacccaca
      541 catcgcggtc gcagtcgacg tcgggcatgc gacggatcat cccgactgcg acaatcgcaa
      601 atacggggag acgaagctgg gcggcggccc catcatctgt cgcggcgcca acatcaatcc
      661 gaagatctat cagcgcctcg tcgcggcggc gaaaaaggcg aacgttccct gtcagctcga
      721 ggccgatccg cgcccgacgg gcaccgatgc acgcgcgatc cagatgggtc gcggcggcgt
      781 ggctacggga ctcgtgagca tcccactccg ctacatgcac acgcccagcg aagtcgtgga
      841 tctcgaggac gtggagcgtt gcgtgaagct cctcgtcgaa ttcgcgaagg cgctcgcgcc
      901 aggcgactac gcgcactggt gagcgttcag gcgccgcgat cgttcaacca atggtccgcg
      961 gcgaggatca agaacacgta gtccgacgtc ggccggccga gacaatattc ggtttgctgc
     1021 cacaggaacg gccaatcgag cagttccgga taatccggcc ggatcagcgc cgtgcccgac
     1081 gcaaccccgc cggggatgta ggaccagtcg ccgcggttta cgccataagc ggtcgtgagt
     1141 gacttcgcac cgacgccgga gacgaacgac gcgttgttcg aacctggatg acagccgagg
     1201 acaaagttga tcgcatgcag catgtagtcg cgcgggaaaa tatcgggtgc acccgcgtgc
     1261 aggaaatatt gctccactcc gaatcgctga atgccccaac ccgcccccca gatgtcgggt
     1321 ttgtagggca gcccgtaggg cgtctctttt tccaacgcgt ccacttttgc gcgatacgtg
     1381 cgcagcgcgg cccggatttt ttcgcg
//
LOCUS       DASPLT010000129         1075 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_185886, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000129 DASPLT010000000
VERSION     DASPLT010000129.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1075)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1075)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1075
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_185886"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1075)
                     /locus_tag="VEA63_01375"
     CDS             complement(<1..>1075)
                     /locus_tag="VEA63_01375"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYD82659.1"
                     /translation="VPVTADDILYWYEMEVKGLEISPPEVLRRPGSADVALVEKIDDL
                     TVRFTFSEPWVLFLERLASYRTIPGFSGSTNYQEYCVPSHYLRQFHPTVGDPDLVAKK
                     MRELSVPTARALYVRMKDLKNPELPRLWPWIARTSMDTSPYVFVRNPYYPAVDTQGNQ
                     LPYLDRIVLDVRPQEVFGLLAASGQVSMQDRYMRYDDHVLLMSQAERNGYSVYQWYSG
                     LRSAFTIFPVLNRKADPARPDTVWKHELLNDRRFRQALSLALNRQDIIDALYNGQGTP
                     AQIDPGPGSPFHSEKLFKSFTEHDPARANALLDELGLTGRDREGYRTFPDGSRMVWYL
                     NLTEVTNNDPAQFIVDDWAAVGIR"
BASE COUNT          212 a          333 c          329 g          201 t
ORIGIN      
        1 gcggatgccg acggccgccc agtcatccac gatgaattgc gcaggatcgt tgttggtgac
       61 ctccgtgagg ttgagatacc acaccatgcg tgaaccgtcg ggaaaggtgc ggtagccctc
      121 gcgatcgcgg ccggtaagtc cgagttcgtc gagcagtgcg ttcgcgcgcg ccggatcgtg
      181 ttccgtgaaa ctcttgaaca acttctcact gtgaaacggc gagcccgggc cgggatcgat
      241 ctgcgccggc gtgccctgcc cgttgtagag cgcgtcgatg atatcctggc ggttcaacgc
      301 cagcgagagc gcctgacgaa aacgccgatc gttcagcaac tcgtgtttcc acaccgtgtc
      361 gggccgcgcc ggatccgctt tgcgattcag cacgggaaaa atcgtgaacg ccgaacgcag
      421 cccggaatac cactggtaaa cgctgtagcc gttgcgctcc gcctggctca tcaagagcac
      481 gtgatcgtcg tagcgcatgt agcggtcctg catcgacacc tgcccgctcg cggcgagaag
      541 gccgaacacc tcctgcggcc gcacatcgag cacgatccgg tcgaggtaag gcagttgatt
      601 gccctgcgtg tcgaccgccg gataataggg attgcggaca aacacgtaag gcgacgtatc
      661 catgctcgtc cgcgcgatcc aaggccacag ccgcggcagc tcgggatttt tcaggtcctt
      721 catccgcaca tacagtgcgc gcgccgttgg cacgctgagc tcgcgcattt ttttcgcgac
      781 gaggtccggg tcgccgacag tcggatgaaa ctgccggaga taatgcgacg gcacgcagta
      841 ttcctgataa ttcgtggagc cgctaaagcc ggggatggtg cgatacgacg cgaggcgttc
      901 gaggaagagc acccacggct cgctgaacgt gaaacgcacc gtgaggtcgt cgattttttc
      961 gacgagcgcg acatccgcgc tgcccggacg ccgcaggact tccggcggcg aaatctccag
     1021 ccccttcact tccatttcat accagtaaag gatgtcgtcc gccgtcaccg gcact
//
LOCUS       DASPLT010000130         1299 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_23089, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000130 DASPLT010000000
VERSION     DASPLT010000130.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1299)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1299)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1299
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_23089"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..653)
                     /locus_tag="VEA63_01380"
     CDS             complement(<1..653)
                     /locus_tag="VEA63_01380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HYD82660.1"
                     /translation="MKRILPLIGLCIPLLGLAAARVSAKEFIERFDPAFDRLVAADAN
                     VEKLSEGYTWSEGPVWYEGAVLFSDVPNNVVYRWAEGMTQAEVFLKPSGLLTEKPGFR
                     EPGSNGLAVDAQGRLLLCQHGERRVARYEKGKFTSLAERIDGKRLNTPNDLAVRKSGE
                     IYFTDPPYGFEGVDNSPMKELDYHGVYRLATDGKVTLLAKTIKYPNGIAFSPDEKVL"
     gene            785..>1299
                     /locus_tag="VEA63_01385"
     CDS             785..>1299
                     /locus_tag="VEA63_01385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007359687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HYD82661.1"
                     /translation="MKFAPAKPPLTGETLDSLTEKLREHGEPAFRAKQILEWLYKKRV
                     RSWDEMTNLSKPLRTWLADTFDLLPAELVLNKQSEDVTDKLLLELRDHSLIETVIIRA
                     PQEGVGLDESRKTICISTQVGCAMACVFCASGLAGLKRDLSAGEIVAQLLQVCYREDA
                     RTPRARMELAS"
BASE COUNT          259 a          495 c          299 g          246 t
ORIGIN      
        1 taaagcacct tctcgtccgg cgaaaacgcg atgccgttcg gatacttgat cgtcttcgcc
       61 agcaacgtga ccttcccatc cgtcgccaga cgatacacgc cgtgataatc cagctccttc
      121 atcggcgagt tgtccacgcc ctcgaaacca tacggcggat ccgtgaaata aatttccccg
      181 ctcttccgca ccgccagatc gttcggcgtg ttcaaccgct ttccgtcgat ccgctccgcg
      241 agcgacgtga actttccctt ttcataacgc gccacccgcc gctcgccatg ctggcacaac
      301 agcagccgtc cttgcgcatc caccgccagc ccgttgctcc ccggctcgcg aaagcccggc
      361 ttctccgtca gcaatccgct cggcttcaaa aatacctccg cctgcgtcat cccctccgcc
      421 caacgataca cgacgttgtt cggcacatcg gaaaacagca ccgccccctc ataccacacc
      481 ggcccctccg accacgtgta accctccgag agcttttcga cgttcgcgtc cgcagccacg
      541 agccggtcga aagccggatc gaatcgctcg atgaactcct tcgcagacac gcgcgcagcc
      601 gccaagccga gcaacggaat acacaaacca atcaggggta gaatacgttt catggtggta
      661 agggattctc cgccctgacg catgagccgc aacgacgttg cgagtcatcc acgcgcgttt
      721 cttttttgcg acttcttgcg cctctttgcg gccactccat cattcttccg tccctttcct
      781 tttgatgaaa ttcgcgcccg ccaaaccgcc gctcaccggc gaaaccctcg actcgctcac
      841 ggaaaaactc cgcgagcacg gggagcccgc gttccgcgcg aaacaaatcc tcgagtggct
      901 ctacaagaaa cgcgtccgct cctgggacga gatgaccaac ctctccaaac cgctccgcac
      961 ctggctcgcc gacaccttcg acctcctccc cgccgaactc gtcctcaaca aacaatccga
     1021 ggacgtcacc gacaaactcc tcctcgagct ccgcgaccac tcgctcatcg aaaccgtcat
     1081 catccgcgcg ccccaggaag gcgtcggcct cgacgaatcc cgcaaaacca tctgcatttc
     1141 cacgcaggtc ggctgcgcga tggcctgtgt tttctgcgcc agcggactcg ccggcctcaa
     1201 gcgcgacctc tccgccggcg agatcgtggc gcagcttctc caagtttgtt atcgcgaaga
     1261 cgcccgcacg ccgcgcgccc gcatggaact cgcgtcctt
//
LOCUS       DASPLT010000131         1103 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_145206, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000131 DASPLT010000000
VERSION     DASPLT010000131.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1103)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1103)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1103
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_145206"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..731
                     /locus_tag="VEA63_01390"
     CDS             <1..731
                     /locus_tag="VEA63_01390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007358998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HYD82662.1"
                     /translation="KVGDTFHPFHGLAFDEESAHAETFTVVGVLEPTNTPADRVIWIP
                     LAGLQTMSGHDARAATEVSAVLVQLRASTAGFMLDMMYNKQGNRLTFAYPIGTIVAEL
                     FQKISWFDRVLELVAYLVALVAAGSVLASIYNSMSARRRDLAILRALGARRRTIFGAV
                     VSEAAVIGALGAVVGLAIYVGLFVVVAAVIRAQTGVVLDAWRWHPVLALCPVAMVALC
                     ALGGIVPALKAYRTPVAETLAPVS"
BASE COUNT          159 a          254 c          459 g          231 t
ORIGIN      
        1 tgaaggtggg cgatacgttt catccgtttc acgggttggc gtttgatgag gagagcgcgc
       61 atgcggagac gttcaccgtg gtcggcgtgc tggagccgac gaacacgccg gcggatcgcg
      121 tgatttggat tccgctcgcc ggtttgcaga cgatgagcgg gcacgatgcg cgggcggcga
      181 cggaagtgag cgcggtgctg gtgcaactgc gcgcgtcgac cgcgggcttc atgctcgaca
      241 tgatgtataa caagcagggg aaccggctga cgttcgcgta tccgatcggg acgatcgtcg
      301 cggaattgtt tcaaaagatt tcctggttcg atcgtgtgct ggagctcgtg gcgtatctcg
      361 tggcgctggt ggcggccggg tcggtgctgg cgagcattta taattcgatg agcgcgcggc
      421 ggcgcgatct ggcgattttg cgcgcgctcg gcgcgcgacg gcggacgatt tttggggcgg
      481 tggtgagcga ggcggcggtg atcggcgcgc tcggcgcggt ggtggggctg gcgatttatg
      541 tgggattatt cgttgtcgtg gcggcggtga tacgcgcgca gacgggggtg gtgctcgatg
      601 cgtggcgctg gcatccggtg ctcgcgcttt gtccggtggc aatggtggcg ttgtgcgcgc
      661 tgggcgggat cgtgccggcg ttgaaagcgt atcgaacgcc ggtggcggag acgctggcgc
      721 cggtgtcgtg aagcggctcc gcgagcgtgg agcgtggcgc gatgagtggc gcaaaatgtg
      781 gggtgggagt gagcgcgcga aaaggggacg aaaagctcac gcttttcgct acggatgggt
      841 tttgttgcgc ggagcgggcg gcggacgagg cgcttggccg gcgcgtgggg acaaggtgct
      901 ctaccggggc gggcaaagtt ttgacggggc gcgatggttg tgcaggtttc gagactcatg
      961 aaaatttcgc gcaaggtttg gctggcggtc gcgtttgggg tgctggcgat ggggcgcttg
     1021 ggcgcggccg atcaggcggc gccggaagcg gagaacgggt atttgaaact tggattcgac
     1081 cggctgtcgg cgttcagctt tgt
//
LOCUS       DASPLT010000132         1225 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_207028, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000132 DASPLT010000000
VERSION     DASPLT010000132.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1225)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1225)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1225
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_207028"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..602
                     /locus_tag="VEA63_01395"
     CDS             <1..602
                     /locus_tag="VEA63_01395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012377066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4861 family protein"
                     /protein_id="HYD82663.1"
                     /translation="GPSRGCGGTGIWDGKQLHVSRNFSKARVLANGPIRAIFELGFDT
                     WAADGIYVTEVKRFIVDAGHNLDLIESTFTATGNRKELTVAIGLNKNPADRGQDPQIA
                     VVRVEADASLAQWVAQKTNGQLGTAVIVAASSFSGYAEDERNLLVLAKTKPGEPLRYL
                     AGAGWSKAGEFTTPEAWNAYVAAQAARLNSPVKVSVGAP"
BASE COUNT          256 a          407 c          341 g          221 t
ORIGIN      
        1 tcggcccgtc gcgcggctgt ggcggcaccg gcatctggga cggcaaacaa ctccatgtga
       61 gccgcaattt cagcaaggcg cgcgtcctcg cgaacggtcc catccgcgcg atcttcgagc
      121 tcggcttcga cacctgggcc gccgacggca tctacgtcac cgaggtgaaa cgcttcatcg
      181 tcgacgccgg ccacaacctc gacctcatcg agagcacgtt caccgccacc ggcaatcgga
      241 aggaactcac cgtcgccatc ggcttgaaca aaaatcccgc cgaccgcggc caggatccgc
      301 agatcgccgt cgtccgcgtc gaggccgacg cttcgctcgc gcagtgggtc gcccagaaaa
      361 ccaacggcca gctcggcacc gcggtcatcg tcgccgcgtc atcattttcc ggatacgccg
      421 aagacgaacg caatctcctc gttctcgcga aaaccaaacc cggcgagccg ctgcgttatc
      481 tcgccggcgc gggctggtcg aaggccggcg aattcaccac acccgaggcg tggaacgcct
      541 acgtcgcggc ccaagccgcg cgtcttaact cgcccgtaaa ggtctccgtc ggcgctccgt
      601 aagggtggag cccgacgtcc cgtcgggcct tgattccctg gagtgcggtg ctttagcccg
      661 caccgaggtt cgaacatgcg gctttgactc cgtttcaggg ggcgaaacaa aaaccctccg
      721 gcagtcgcag tcaccggaga gtatgttcag ttgaaccgcc cagcaaacta cagcggctcg
      781 aaaagcccta tagccaaatc tttggccgca aagaggcaca aaaggcgcaa gaacagaagc
      841 gtcctccgaa tcgtttttag caccttctgc gccttttggc tgctatagtt tcaattgatc
      901 gctctaaacg gagtgtagag ccggaccttg cgtccgcgcc gcttcgcccg caaatcgggc
      961 gccgacaagc ggcgccccta catcctcact gtatcgctca tctgagctgt attcatgcag
     1021 gagacctccc gcgaatgaac gcgaatttca gagaagtgga atcaaatgta gccgcatcaa
     1081 ggccagatca tcgttgggtg aaagatcatt tctcttcgct tgtattcgcg acaattggcg
     1141 tgattcgcgg atcaactgaa tgatcccgct agaacgcacg gcacctccgc gacccgcctg
     1201 acctggcgcg cggcgcgcgg atgaa
//
LOCUS       DASPLT010000133         1421 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_267905, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000133 DASPLT010000000
VERSION     DASPLT010000133.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1421)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1421)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1421
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_267905"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..482
                     /locus_tag="VEA63_01400"
     CDS             <1..482
                     /locus_tag="VEA63_01400"
                     /inference="COORDINATES: protein motif:HMM:NF037199.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF5597 domain-containing protein"
                     /protein_id="HYD82664.1"
                     /translation="LAKAYAVLRDLSPLILEHQAHGSLAAAWLSRDTTKQEIALNGYT
                     VDIELRRNRRTGEPAADLGYAMIFALGPDEFHVTGTDVQVTFRPRTDGPPIAGIADAE
                     AGIYRDGRWQPRRKMSGDDILLDYKLAAAAAANQSGSGLLFGPDGPTSQRVKLYRYR"
     gene            479..>1421
                     /locus_tag="VEA63_01405"
     CDS             479..>1421
                     /locus_tag="VEA63_01405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonan acetylesterase"
                     /protein_id="HYD82665.1"
                     /translation="MNFPSVTRVLFLFSALALRLSLSADDAAHTFDDSLPPPAFSVAV
                     PKGNHRVTIEFGANDAASDTTVRAESRQLFIEHLTTRPGEFVTRTFIVNVRTPRLPPP
                     ERNAPSGTAVLLNERERDLLRWDDQLTLEFTGSAPRVRSIAIEPAPADTPTIFLAGDS
                     TVTDQPHEPAASWGQMLPRFFKPAVAIANHAESGETLKSFLSGLRLAKILSQIKPGDY
                     LFLQFGHNDQKKNWPQTYAEARTTYTAYLRAYIAEARLRGATPILVTSPQRRTFDEHG
                     RIKNTHGDYPDAVRALARAERVALLDLERASIAFYEAL"
BASE COUNT          261 a          601 c          330 g          229 t
ORIGIN      
        1 ctctcgccaa agcctacgcc gttctccgcg atctctcgcc cctcatcctc gaacaccagg
       61 cgcacggctc cctcgccgcc gcttggttgt cgcgtgacac aaccaaacag gaaattgcgc
      121 tcaacggcta caccgtcgac atcgaactcc gccgcaatcg ccgcaccggc gaacccgccg
      181 ccgatctcgg ctacgcgatg atcttcgccc tcggccccga tgaatttcac gtcaccggca
      241 ccgacgttca ggtcacgttc cgcccccgca ccgatggccc gccgatcgcc ggcatcgccg
      301 acgccgaggc cggcatttat cgcgatggtc gctggcaacc tcgccgcaaa atgagcggcg
      361 acgacatcct gctcgactac aaactcgccg ccgccgcagc cgcgaatcag tccggcagcg
      421 gactcctctt cggccccgac ggcccgacct cccaacgcgt caaactctac cgctaccggt
      481 gaacttcccc tccgtcaccc gcgtcctttt tctcttctcc gcgctcgcgc tccgtctatc
      541 tctctccgcc gacgacgccg ctcacacgtt cgacgacagc cttccgccgc cggcattttc
      601 cgtcgccgtt cccaaaggaa accatcgcgt cacgatcgaa ttcggcgcca acgatgccgc
      661 ctccgacacc accgtccgcg ccgagtcgcg ccagctcttt atcgagcatc tcacgacccg
      721 cccgggcgaa ttcgtcacgc gcaccttcat cgtcaacgtc cgcacaccgc ggcttccgcc
      781 gcccgagaga aacgctccca gcggcaccgc cgtcctcctt aacgagcgcg aacgcgacct
      841 ccttcgctgg gacgaccaac tcacgctcga gttcaccggc tccgcgccac gcgtgcgctc
      901 catcgccatc gaacccgcgc ccgccgacac acccacgatt ttcctcgccg gcgactccac
      961 cgtcaccgac cagccgcatg agccggccgc aagctggggc cagatgctcc cgcgcttttt
     1021 caaacccgcc gtcgcgatcg cgaaccacgc tgaatccggc gaaacgttga aatcctttct
     1081 ctccggcctc cgcctcgcga aaatcctcag ccagatcaaa cccggcgact acctcttcct
     1141 tcaattcggc cacaacgacc aaaagaaaaa ctggccgcaa acctacgccg aagcccgcac
     1201 cacctacacc gcctacctgc gcgcctatat cgccgaagca cgtctccgcg gcgcgacgcc
     1261 cattctcgtc acctcgccgc aacgccgcac cttcgacgaa cacggtcgca tcaaaaacac
     1321 gcacggcgac taccccgacg ccgtccgcgc tctcgcgcgt gccgagcgcg tcgcgctcct
     1381 cgatctcgaa cgcgccagca tcgctttcta cgaagcgctc g
//
LOCUS       DASPLT010000134         2278 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_42745, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000134 DASPLT010000000
VERSION     DASPLT010000134.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 2278)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2278)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2278
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_42745"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..822)
                     /locus_tag="VEA63_01410"
     CDS             complement(<1..822)
                     /locus_tag="VEA63_01410"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HYD82666.1"
                     /translation="MTTELAPELQELLRVGDRIAAVGEPSAGAFDVLYAPFGRSALMS
                     AGVPTVSLIVDLLHRDLPAALPIEEINYRHEWFSQIATVATSFQCISHYTAERLQAHY
                     GVDPRRCVVTHIPVHTRLVSTDDRAAAVPPPVNGPFFFYPANFWPHKNHATLLAAYRD
                     YCDNRPGAAWPLVFTGHPDASMHALVAQAAAMGLDGRVRFLGHVSEDELVAVWRAAGA
                     LVYPSLHEGFGIPLLEAMAFGCPIIASNTTSMPEVGGDACEYVDPRDARSIAEALR"
     gene            1346..>2278
                     /locus_tag="VEA63_01415"
     CDS             1346..>2278
                     /locus_tag="VEA63_01415"
                     /inference="COORDINATES: protein motif:HMM:NF037997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015114 - phosphate ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi symporter"
                     /protein_id="HYD82667.1"
                     /translation="MLLINIAAGVALMLFGVRFLRKGLDRLFGRSLHTWLDRIAHSRG
                     RAAFAGACVGIVAPSSTAQTLLSLQLLGSGRLSIERTLVFLLAANAGISATVQLIALR
                     VFDYFGVFLVPGLAGFYLLRSPRRRGAAQVCLALGFIFLGMNLVSEQARELAAGPTFA
                     TLLGLLAEQSVMVALLAAGFTVACQSSTACIGLALGLTASGHVSLALLVPVIVGANLG
                     MGVTSVLASYTTREGRWLGAMNLGLKTVLAVAALAALQSLTALLANTPGDLTRQAANF
                     HTAFGLAVAALGFALARPLGALARRELNRHRTDAA"
BASE COUNT          380 a          802 c          663 g          433 t
ORIGIN      
        1 gcgcaacgcc tccgcgatcg aacgcgcatc gcgcgggtcg acatactcgc acgcatcacc
       61 gccaacttcg ggcatcgacg tcgtgttcga tgcaatgatc ggacatccga acgccatggc
      121 ctcgagcaag ggaatgccga aaccttcatg cagcgaggga tacaccagcg ccccagcggc
      181 gcgccacacg gcgacaagtt catcctcgct cacatgcccg agaaaccgaa cgcgtccgtc
      241 gagccccatc gccgccgcct gtgcgacgag cgcatgcatg ctcgcatccg gatgtccggt
      301 gaacacgagc ggccaggccg cgcccggccg gttgtcgcaa taatctcgat acgccgcgag
      361 cagtgtcgcg tgatttttgt gcggccagaa atttgccgga taaaaaaaga acggtccgtt
      421 caccggcggc ggaacagccg ccgcgcgatc gtccgtcgaa acgaggcgcg tgtggaccgg
      481 gatgtgcgtc acgacgcaac gccgcggatc gaccccatag tgcgcttgca gccgctccgc
      541 cgtatagtgc gagatgcatt gaaacgacgt cgccaccgtc gcgatctggc tgaaccattc
      601 gtgccggtag ttgatctcct cgatcggcag cgccgccggc aaatcgcggt gcagcaaatc
      661 cacgatcaaa ctcaccgtcg gcacgcctgc gctcatcagc gccgatcggc caaacggcgc
      721 gtataaaacg tcaaacgcgc ccgccgacgg ctcgcccacg gccgcgatcc ggtctcccac
      781 ccgcagtaat tcttgcagct ccggtgcgag ttccgtcgtc acgaaataaa caaagttaaa
      841 ccgcttcggt tgcgccctcg cgatctctcg caagaacgaa aataccagtg gcttcacgcc
      901 tccgttcgcg ccacgcggta gcatcaacaa caaatccaca ccgaccacaa tcggcggatc
      961 gttgcgccca aacacgcgcc gcagccgtgc gaaccaggag gaccggcgcc gtgcctccga
     1021 tgtccttcgc tcgtcttgag ccgcctcggc ctcgcgcgcg cgttccgcag aagtgagcac
     1081 gggatcgttc gatggcggcg gcatggcgtc ggccgcgaaa ttggggcggc ggtgtcggct
     1141 tgtccactcg cgagcgccaa gccggccgcc gatccgatca aacgcctccc cgattcccgt
     1201 aaaccgaccg ccgtggtttt cacgtggtca ccgacaatct tcctcgcaac gcgggaacga
     1261 gttcgccgcg tccggtctat cggtctcatg cgcttggttt cacggtcgat tcctcattct
     1321 tctcttcgta tgtccgcgta gcgccatgct tctcatcaat atcgccgcgg gtgtagccct
     1381 catgcttttc ggagtgcgat ttctccgcaa aggcctcgat cgtttgttcg gacgatcgtt
     1441 gcacacctgg cttgatcgga tcgcacacag ccgcggtcgc gcggctttcg ccggtgcctg
     1501 cgtcggcatc gtcgcgccgt catccaccgc acagacgctg ctttcgttgc aactgctcgg
     1561 ctcgggtcgg ctctcgatcg agcgcacgct cgtcttcctg ctcgccgcca acgcggggat
     1621 ttccgccacc gtgcaactca tcgcgttgcg cgtgttcgac tacttcggcg tttttctcgt
     1681 ccctggcctc gcgggattct acctgctgcg ttcgccgcga cgccgcggcg cggcgcaggt
     1741 gtgccttgcg ctcggtttca tctttctggg gatgaatctc gtcagcgaac aagcacgcga
     1801 actcgccgcc ggcccgacgt tcgccacgct gctgggcctc ctcgcggaac aaagcgtgat
     1861 ggtcgctctg ctcgcggcgg ggttcaccgt agcatgccaa agctcgaccg cgtgcatcgg
     1921 cctcgcgctc ggcctcacgg cgtccggtca cgtttctctt gcgcttctcg ttcccgttat
     1981 cgtcggagcc aatctcggca tgggcgtcac ctcggtcctc gcaagttaca cgacgcgcga
     2041 aggccgctgg ctcggcgcga tgaacctcgg tctaaaaaca gtcctcgctg tggcggcgct
     2101 tgctgcgctg cagtcgctca ccgcgttgct cgcgaacacg cccggcgatc tcacgcgaca
     2161 ggcggcgaat ttccataccg ctttcggcct cgcggtcgcc gcgctgggct ttgcgctcgc
     2221 ccgtccgctc ggtgcgctgg cccggcgcga gttgaaccgc caccgcaccg acgcagct
//
LOCUS       DASPLT010000135         1006 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_207050, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000135 DASPLT010000000
VERSION     DASPLT010000135.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1006)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1006)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1006
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_207050"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1006)
                     /locus_tag="VEA63_01420"
     CDS             complement(<1..>1006)
                     /locus_tag="VEA63_01420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007358665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HYD82668.1"
                     /translation="AILDKLQLKSPANRTAMNETDALNFAKEIGFPVLLRPSFVLGGR
                     GMFIVYSEDEFRAVVRQAFDVMPDKPVLIDKFLEDAIELDVDCISDGETSVIGGMLEH
                     IEFAGVHSGDAAMVMPPHTLSPDMLDIVRTATYALARELNVVGLMNVQFAIKDNQLYV
                     LEVNPRASRTVPFVAKAIGVPLAKLAAKVMTGVKLKDLGYTREQTPKHWCVKEAVFPF
                     VRFPGATIALGPEMRSTGEVMGLDADLGIAFAKAQAAAKPGLPTAGNVFLSVKESDKT
                     RAIDLARRLEAMGFALYSTSGTAQVLSDSGIAVKRLSKINEGRPTAVDLIKNGQIHMV
                     I"
BASE COUNT          181 a          348 c          290 g          187 t
ORIGIN      
        1 tgatcaccat gtgaatctgg ccgttcttga tcaagtcgac cgccgtcggg cggccttcgt
       61 tgatcttcga aagccgtttc acggcgatgc cgctgtcgct taacacctgc gcggtgccgc
      121 tcgtcgaata gagcgcaaaa cccatcgcct cgagccggcg cgcgagatcg atcgcgcgcg
      181 tcttgtccga ctctttcacc gacaggaaca cgttgcctgc ggtcggcaac cccggtttcg
      241 ccgccgcctg cgctttggcg aatgcgatgc cgagatcggc gtcgagaccc atgacttcgc
      301 cggtcgaacg catctcgggt ccgagcgcga tcgtcgcccc gggaaaccgc acgaagggaa
      361 acaccgcttc cttcacgcac caatgcttcg gcgtctgctc gcgcgtgtaa ccgaggtcct
      421 tcagcttcac gcccgtcatc accttcgccg cgagctttgc cagcggcacg ccgatcgcct
      481 tcgccacgaa cggcaccgtg cgcgatgcgc gcgggttcac ctcgagcacg taaagctggt
      541 tgtccttgat cgcgaactgc acgttcatca ggccgacgac gttcaactcg cgcgcgagcg
      601 cgtaggtcgc cgtgcgcacg atatcgagca tgtccggcga aagcgtgtgc ggcggcatca
      661 ccatcgccgc gtcgcccgag tgcacgccgg cgaactcgat gtgctcgagc atcccgccga
      721 tcacggaggt ctcgccatca ctgatgcaat cgacgtcgag ctcgatcgca tcctcgagga
      781 acttgtcgat cagcaccggc ttgtccggca tcacgtcgaa cgcctgccgc accaccgcac
      841 ggaattcgtc ctcgctgtaa acaatgaaca tgccgcggcc accgagcacg aacgacggcc
      901 ggagcagcac cgggaaaccg atctccttcg caaaattgag cgcgtccgtt tcgttcatcg
      961 ccgtgcgatt cgccggcgac ttgagctgca gcttgtcgag aatcgc
//
LOCUS       DASPLT010000136         1190 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_2051, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000136 DASPLT010000000
VERSION     DASPLT010000136.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1190)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1190)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1190
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2051"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..362)
                     /locus_tag="VEA63_01425"
     CDS             complement(<1..362)
                     /locus_tag="VEA63_01425"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HYD82669.1"
                     /translation="MCCGTMTFGAQTDEATAAEMVSYCLDHGINFFDTANVYNQGASE
                     AILGRLLKGRRDRVVLASKVAGKMGPAPDQAGLSRAAIVRAVEESLQRLQTDHLDIYY
                     LHWPDYAVPLEESLGALQ"
     gene            complement(418..>1190)
                     /locus_tag="VEA63_01430"
     CDS             complement(418..>1190)
                     /locus_tag="VEA63_01430"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HYD82670.1"
                     /translation="VPGYPQPARRISATDLLAKAAELNVHVVQIADNLPLHHLDDGEL
                     AALAQRATTMGIELELGTAGIDADNLRRYLAIAQRLNARVLRTVIDAGDERPSPAQVV
                     ARLAPLALEFERAGVTLAIENHDRFKATTLRDIVERIDSAHIGICLDTSNSLGCMEGP
                     EHVVETLGRYVVNLHIKDVRVFRPPHHKGFVVEGRPAGQGQLDIPALLARLRAFGVDP
                     NAIVELWPPPERDVADAVAKEEQWTRESVDYLRPLIPG"
BASE COUNT          208 a          416 c          352 g          214 t
ORIGIN      
        1 tcctgcagcg cgccgagcga ttcctcgagc ggcaccgcgt agtccggcca atgcagataa
       61 taaatatcca ggtggtcggt ctggagccgc tgcaagcttt cctcgaccgc gcgcacgatc
      121 gccgctcgcg acaaacccgc ctgatcgggc gccggaccca tcttgccggc gaccttgctc
      181 gcaagcacca cgcgatcccg ccggcccttt agcaagcggc cgagaatcgc ttccgacgcc
      241 ccttggttgt agacgttcgc cgtatcgaaa aagttgatgc cgtgatccag acagtagctg
      301 accatttcgg cagccgtggc ctcgtcggtt tgcgcgccga acgtcatcgt gccgcagcac
      361 acgcgtgaaa cgttcagtcc agttttgtga agtgtcctcg tttccataaa aaatcgatca
      421 gccgggaatg agcggacgca ggtagtcgac gctttcccgc gtccactgtt cctccttcgc
      481 gaccgcatcc gccacgtccc gctccggcgg cggccacagt tcgacgatcg cattcggatc
      541 caccccaaac gcacgcagcc gcgcgagcag cgccgggatg tccagctgcc cttggcccgc
      601 gggccgcccc tcgacgacaa accccttgtg atgcggcgga cggaacacgc gcacgtcctt
      661 gatgtgcagg ttgacgacgt agcggcccag cgtctcgacg acgtgctcgg gtccttccat
      721 gcagccgagt gagttcgagg tgtcgagaca gatgccgatg tgcgcactgt caatccgctc
      781 gacaatgtcg cgcagcgtcg tcgccttgaa ccgatcgtga ttttcgatcg ccagcgtcac
      841 gcccgcgcgc tcgaactcca gcgccagcgg cgccagccgc gcgacgactt gtgcgggcga
      901 aggcctttca tcgcccgcgt caatcaccgt gcgcagcacg cgcgcgttca accgctgcgc
      961 aatcgcgaga taacggcgaa gattgtccgc gtcgatcccc gccgtgccga gctcgagttc
     1021 gattcccatc gtcgtcgcgc gctgcgcgag cgcggccagt tctccgtcat cgagatgatg
     1081 cagcggcagg ttgtccgcga tctgaaccac gtggacgttc aactccgccg ctttcgcgag
     1141 caggtcggtc gccgaaatcc gacgcgccgg ctgcggataa cccggcacgc
//
LOCUS       DASPLT010000137         1892 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_2061, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000137 DASPLT010000000
VERSION     DASPLT010000137.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1892)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1892)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1892
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2061"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            436..810
                     /locus_tag="VEA63_01435"
     CDS             436..810
                     /locus_tag="VEA63_01435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD82671.1"
                     /translation="MRTFLDLYCEKNNCSPEQFHRHVFRHCLYAHARPLAPLIHLFNY
                     EFFAPDRTLILSVAGAVTMKRVREEVRDYFWDSNNRGWLRESLRIRMSGQRLRELARE
                     YLPESSSAPPFSPPESGSRSPL"
     gene            complement(1029..>1892)
                     /gene="meaB"
                     /locus_tag="VEA63_01440"
     CDS             complement(1029..>1892)
                     /gene="meaB"
                     /locus_tag="VEA63_01440"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HYD82672.1"
                     /translation="LAQQLLAKLLPYTGGSRRIGITGIPGAGKSTFIETFGCQLIADG
                     HRVAVLAVDPSSSVSGGSILADKLRMEKLSRESGAFIRPSPSGGSLGGVTRKTREAIL
                     VCEAAGFDVVIVETVGVGQNEVAVRSMVDFFLVLMIAGAGDEMQGIKKGVIELADALV
                     INKADGENRMRATAAQAEMQRVLHYLQPTTPGWPTVAALASALTGDGVAEVWRIAEAF
                     FATARASGELERRRRVQAVEWMHALIGESLRTRFYASALVRDRLPGVEKAVAAGTTPP
                     LAAALELLRLE"
BASE COUNT          310 a          715 c          521 g          346 t
ORIGIN      
        1 gccgaatatc gcgccgtgcg ccgccgcgag ctcgaagacg cgcattggcg cgaaatgatc
       61 gcccgtttct acgagccgcc gccgcgcgag ccccccgtcc tgcagcgcta acgcattcct
      121 tcgaagcgtc gccggtttca agggcgcagg cgccgcgttc cgctgtgtta tgacgcgctt
      181 agctccagct cgatcgcggc attccgtcca gcccgcgctc cgttaacccc tcgtgcttcg
      241 agccgcggcc tactgccgca agccgcagtt tgaccgaggc ttgaaagcgg cccgccgctc
      301 gtcggttatt ttccccgacg cgcaagccgc gctggcttct tcgccggccg cgcgttgcgc
      361 aggcgcccgg tgatccggtg tgttgaatgt ttgcacttct ttgcccgagt cctgcttatg
      421 ggcggccctg cttcgatgcg cacgttcctg gacctttact gcgaaaaaaa caactgctcg
      481 cccgagcaat ttcaccgcca tgttttccgg cactgcctct acgcgcacgc ccggccgctc
      541 gcgccgctga ttcacctctt caactacgaa ttcttcgctc ccgatcgcac gttgatcctc
      601 agcgtcgccg gcgccgtcac gatgaaacgc gtgcgcgaag aagttcgcga ctatttctgg
      661 gattccaaca accgcggttg gctccgggag tccctgcgca ttcgcatgtc cggccagcgc
      721 ctgagagaac tcgcgcgcga atatctcccc gaaagcagct cggccccgcc gttctccccg
      781 cccgagtccg gctcacgttc tccgctgtga agaagtaacg gcgctggcag ccccgcttga
      841 ttcgcttgaa cgcgttcgtc gttaacgcgg aaatttcaca ctcgcgccgc ggtgccaaag
      901 gtagggacgc ctctccgagg cgtccgcgga cggctcggag agccgccccc accttcgcga
      961 aaaacacttc gtaacgtcga ttctagtgag cgggagatat tccgggttgg cgcgggtcgc
     1021 gcgcgcgatt actccagcct cagcaactcc agcgccgccg cgagcggcgg cgttgttccc
     1081 gccgccaccg ccttttccac gccgggcagt cgatcgcgca ccagcgccga cgcataaaac
     1141 cgcgtgcgca agctctcccc aatcagcgcg tgcatccact cgaccgcctg cacgcggcga
     1201 cgtcgttcga gttcgccgct cgcccgcgcc gtcgcgaaaa acgcttccgc gattcgccac
     1261 acctccgcca caccgtcgcc ggtcaacgcc gacgcgagcg ccgccaccgt cggccaaccc
     1321 ggcgtcgtcg gctgcaaata gtgcaacacg cgctgcatct ccgcctgcgc cgccgtcgca
     1381 cgcatccgat tctcgccgtc cgctttgttg atcaccagcg catcggccag ctcgatcacg
     1441 cctttcttga ttccctgcat ctcgtcgccc gcgcccgcga tcatcagcac gagaaaaaaa
     1501 tccaccatcg accgcactgc cacttcgttc tgacccacgc cgaccgtctc cacgatcacg
     1561 acgtcgaagc ccgccgcttc gcacaccaag atggcttcgc gcgttttccg cgtcacgccc
     1621 ccgagcgacc cgcccgacgg cgacggacgg atgaacgcgc cggattcacg gctgagtttc
     1681 tccatgcgca acttgtccgc gaggatgctt ccgccggata cgctgctcga cggatccacc
     1741 gccagcaccg cgacgcgatg gccgtcggcg atcaactgac aaccgaacgt ctcgatgaac
     1801 gtgctcttgc ccgctcccgg aattcccgtg atgccgatcc gccgcgaacc gcccgtatac
     1861 ggcagcagct tcgcgagcaa ctgctgcgcg ag
//
LOCUS       DASPLT010000138         2170 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_145332, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000138 DASPLT010000000
VERSION     DASPLT010000138.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 2170)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2170)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2170
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_145332"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..374)
                     /locus_tag="VEA63_01445"
     CDS             complement(<1..374)
                     /locus_tag="VEA63_01445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012377368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HYD82673.1"
                     /translation="MSFAPKQKAIDQSLAPAAFASDIEAFLGYISLERGLSKNTIAGY
                     RRDLDQAAAFLVRHGAADWRAVTGEQAAAWIRSLSADDYTVASLARKLSALRNLARFL
                     VREKIRDDDFSALLSGPKLTRR"
     gene            complement(569..832)
                     /gene="infA"
                     /locus_tag="VEA63_01450"
     CDS             complement(569..832)
                     /gene="infA"
                     /locus_tag="VEA63_01450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003130554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HYD82674.1"
                     /translation="MADGNSIEVEGRVVAVLPGTMFKVQLSNGHVVLAHISGKLRKNF
                     IKIAAGDMVKMEMSPYDLEKARITFRMKETNSNYTPPPRRRQY"
     gene            976..1500
                     /locus_tag="VEA63_01455"
     CDS             976..1500
                     /locus_tag="VEA63_01455"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012377370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0052717 - tRNA-specific adenosine-34
                     deaminase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HYD82675.1"
                     /translation="MSDPAAPLPCPFEKRFPSQLVRDDAFFMSLAYNQAIDAWRLDEV
                     PIGAVIEVDGEVIGSAHNTVESAHDPTAHAEMLAITQAATKLGDWRLEGATVYVTKEP
                     CPMCSGAMLMSRVKRVCYAVPDPKMGCLGGATNLNDLPRVNHHVELTAGGVLESECRE
                     LLQAFFKLKRSALE"
     gene            1636..>2170
                     /locus_tag="VEA63_01460"
     CDS             1636..>2170
                     /locus_tag="VEA63_01460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013352894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-Mn family superoxide dismutase"
                     /protein_id="HYD82676.1"
                     /translation="MAYELPKLPYAYDALAPHIDAKTMEIHHTKHHQAYITNANNLLK
                     DHADLAALDVNELIKDLSRVPEAIRGGVRNNAGGHSNHAFFWKILGPGKGGEPKGKLA
                     AAIDAELGGFAKFKEDFAKAAATRFGSGWAWLVVGAGGKLAVGSTANQDSPLMGKAVA
                     GFEGKPVIGLDVWEHAYY"
BASE COUNT          424 a          747 c          591 g          408 t
ORIGIN      
        1 atccgccgcg tgagcttcgg gcccgagagc aacgcgctga aatcgtcgtc gcgaattttc
       61 tcgcgcacga gaaaccgcgc gagatttcgc agcgcgctta attttcgcgc gaggctcgcc
      121 acggtgtaat cgtccgcgct cagcgagcga atccacgccg ccgcctgctc gcccgtcacc
      181 gcacgccagt ctgccgctcc gtgacgcacg agaaacgccg ccgcttggtc gagatcgcgc
      241 cgatagcccg cgatcgtgtt cttcgacagc ccgcgctcga gactgatgta accgagaaac
      301 gcctcgatgt cgctcgcgaa cgccgccggc gccaaactct ggtcgatcgc tttctgcttc
      361 ggggcaaaac tcatctcgct gcaaccgtgc acacgcgaaa tccccgcggc gagcccgttc
      421 agcaaaggtg gaacccgacg tcccgtcggg ttttttgttc aaccgcgacg atcgccacgc
      481 gatcactcgc ccatgcaccc acgctcacct tcgcacttcg gccgcgtctt cgctccgacg
      541 cacgcgcgat cagcgcgtca ccattttctc aatactgccg gcgccgcggc ggcggcgtgt
      601 agttcgaatt cgtttccttc atgcggaagg tgatgcgcgc cttctccaga tcgtaagggc
      661 tcatctccat tttcaccatg tcgccggcgg cgatcttgat gaagtttttc cggagctttc
      721 ccgaaatatg cgcaagcacg acgtgtccgt tcgacagctg caccttgaac atcgtgccgg
      781 gaagcaccgc tacgacgcga ccttctactt caatcgaatt gccgtcagcc atggtggttg
      841 cgaacaggcg agttgccaga ggtgcgttgg atcatgtggt cgtgcgaagg aataaaagcc
      901 ccttgtaagg cggaaacgcc acccctaggc aaggctttac cttgggcaat tcgcccgcca
      961 tcgcacgtca cctccatgtc cgatcccgcc gcgccgctcc cttgcccgtt cgaaaaacgc
     1021 ttcccttccc aactggttcg cgatgacgcc tttttcatga gcctcgccta caatcaggcg
     1081 atcgatgcgt ggcggctcga cgaggtcccg atcggtgcgg tcatcgaggt cgatggcgaa
     1141 gtgatcggct ccgcgcataa caccgtcgaa agcgcccacg atcccaccgc ccacgccgag
     1201 atgctcgcga tcacccaagc cgccacgaag ctcggcgatt ggcggctcga aggcgcgacg
     1261 gtttacgtca ccaaggagcc ttgtccgatg tgctccggcg ccatgctgat gtcgcgcgtc
     1321 aaacgcgtct gctacgccgt ccccgatccg aaaatgggct gcctcggcgg cgcgacgaat
     1381 ctcaacgacc ttccccgcgt gaatcaccac gtcgagctca ccgccggcgg cgtgctcgag
     1441 agcgagtgtc gcgaactcct ccaagctttt ttcaaattga aacgcagcgc actggaatag
     1501 cgggtcatct tccgtagcga aacgcgtgag cgtttcgtga ttcccccgcg gcataaatcc
     1561 gcccgcgcag ttgacgatgt ccgcgagccg acccactttg cccctttccc atcaaccagt
     1621 aaccatagaa atatcatggc ctacgaactg ccgaaactcc cttacgccta cgacgccctc
     1681 gcgcctcata tcgatgcgaa gacgatggaa atccatcaca cgaagcatca ccaggcctac
     1741 atcacgaacg cgaacaacct cctcaaggac cacgccgacc tggctgcgct cgatgtgaac
     1801 gaattaatca aggacctcag ccgcgtcccc gaggcgattc gcggcggcgt gcgcaacaac
     1861 gccggcggcc actccaatca cgccttcttc tggaagattc tcggtcccgg caaaggcggc
     1921 gaacccaagg gtaaactcgc cgcagcgatc gacgccgaac tcggcggttt cgccaagttc
     1981 aaggaagact tcgccaaagc cgccgccacc cgcttcggct ccggctgggc ctggctcgtc
     2041 gtcggtgcgg gtggcaagct cgccgtcggt tcgaccgcca atcaggacag cccgctcatg
     2101 ggcaaagccg tcgctggctt tgaaggcaag ccggtcatcg gtctcgatgt ctgggaacat
     2161 gcctactacc
//
LOCUS       DASPLT010000139         1177 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_23356, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000139 DASPLT010000000
VERSION     DASPLT010000139.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1177)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1177)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1177
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_23356"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(149..505)
                     /locus_tag="VEA63_01465"
     CDS             complement(149..505)
                     /locus_tag="VEA63_01465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD82677.1"
                     /translation="MKTLTTLEHPAVAATSRTLLSKHGFACALTTLQPSEGATAIDAA
                     QGGEEHLLFVVDGEVSVRIGEITTLLKKEEAFLLPKEQTCSVSAFGQRPAKVLRVDVP
                     PRQVVEPPIYAFESAK"
     gene            785..>1177
                     /locus_tag="VEA63_01470"
     CDS             785..>1177
                     /locus_tag="VEA63_01470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012377359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HYD82678.1"
                     /translation="MDGIDLIQDLAIVLLAAGLAGALCRRLGLSVIVGYLVAGTIIGP
                     YTPPFSFIQDVDRIQMLSQVGLVFLMFAIGLGLSISKLGRMGAPTLLATGVGALLMLF
                     LTQALGRAIGWAPMQATFMAAMFMVSSSA"
BASE COUNT          208 a          384 c          350 g          235 t
ORIGIN      
        1 acagagctgc agcgtccact ttcccgccat cggcggaaaa gcgcagaaca aaggttgact
       61 ggctgcaggg aacttcaagc gacgacacca ctgccgccgt cgtcgccgtt gtcaacggcc
      121 aaggtctttt cgtcccactc cgtcgccgtt acttcgccga ctcgaaagcg tagatcggcg
      181 gctcgaccac ctgacgcgga ggaacatcga cgcgcaggac tttcgccggc cgctgcccga
      241 aagcggaaac ggaacacgtc tgttccttcg gcagcagaaa cgcctcttcc ttcttcagca
      301 gcgtcgtgat ttcgccgatg cgcaccgata cttcgccgtc gacgacgaaa agaaggtgct
      361 cctcgccacc ttgggcggca tcgatcgcgg tggcgccctc cgacggttgc aacgtcgtca
      421 acgcgcacgc gaatccgtgc ttcgaaagca gggttcggga ggtggccgcg acggccgggt
      481 gctcgagagt cgtaagtgtt ttcatgatac agacggataa ggttcgttga acggtcggtt
      541 cggttggtgg cacgccggag cgcgcagccc cgaggagcgg agctcgcccg ggcgaaatcg
      601 ggttcacgca acttatctcc gctgcgagta tgtcaagccg cgcgcgcctc cgggccgttc
      661 gaagttcgac tttaaaaaat cgcatggttc gcagccgtgg catcgcccaa gctcgacaca
      721 tggcaacgac atcgaatgtt gatgtcgcca ccgcacaatt tcgtgccggc accccctccc
      781 gctcatggac ggaatcgatt taattcaaga tctcgcgatc gtgctgctcg ccgccggcct
      841 cgctggcgca ctctgccggc gactcggttt atccgtcatc gtcggctacc tcgtggccgg
      901 aaccatcatc ggcccttaca cgccgccgtt ctccttcatt caggacgtcg atcgcatcca
      961 gatgctgtcg caggtcggcc tggtgttcct gatgttcgcg atcgggctcg gcttgagcat
     1021 cagcaaactc ggtcgcatgg gcgctcccac gctgctcgcc acaggggtcg gcgcgctgct
     1081 gatgttgttt ctcacgcagg cgctcggccg cgccatcggc tgggcgccca tgcaagccac
     1141 gttcatggcc gcgatgttca tggtctccag ttcggcg
//
LOCUS       DASPLT010000140         1179 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_124632, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000140 DASPLT010000000
VERSION     DASPLT010000140.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1179)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1179)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1179
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_124632"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            362..>1179
                     /locus_tag="VEA63_01475"
     CDS             362..>1179
                     /locus_tag="VEA63_01475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD82679.1"
                     /translation="MIAFLAVVTAVYFFDREKGGNLRQDRLEREAVIERKAAYIGDQL
                     GNAVNQRIGAMASGELQFTPLEDSVSARTLAAALDTVTARYIGLTAMSAVYPSGRISR
                     GAGALLGEAGMQPHVDTALGNAFKRAETTRQPAATAVVTAAQNVRRVVIFDPIESNEK
                     LLGYLAAELDPALIYRNVIQQQEVADSLTSGNIPTYHAVYGPNRALISPPLAAPRGWP
                     TTTRPIRVVDTEWLVEVAYQPVDPRFYRGQQIMRWAIGLLIAVFTALFFLFLRRT"
BASE COUNT          224 a          363 c          366 g          226 t
ORIGIN      
        1 cttgcatgac caattgcaac ttccgtttga caccctcgct cgttgcggga tcggcgatta
       61 catccgggat ttcgcggcgg cgtcccagga tcttcgcttc ttcgatgcct gcgcgaatga
      121 tgtacatcgg cgagcacgtg agcgaggccg cgcagacggc gactaagagg atgagcagaa
      181 aggccttacg ctttgacatg cccggccaag atgggccgga gcgacagatg tcgccagcgc
      241 gtgacttttg gtacgggtag ggtatagttt ctgcaccatg cggcggcaaa tgacgtcacc
      301 cgctcccccc aggcacggcg ccttcacgtg gatcaccgga aaatacggtg atctcatacc
      361 tttgatcgcg tttttggcgg tagttaccgc cgtttacttc tttgatcgcg agaagggcgg
      421 caatctccga caggaccggc tcgagcggga agcggtgatc gagcgcaagg cagcctacat
      481 tggtgaccag ttaggcaatg cagtgaacca gcgcatcggc gcgatggcat cgggcgagct
      541 gcagttcaca ccgctggaag actcggtctc agcgcgcacc ctggctgcgg cattggatac
      601 ggtgaccgcg cggtacatcg ggctcaccgc gatgtccgca gtgtatccga gcgggcgcat
      661 aagccgcggc gcgggcgcac tgttgggcga ggccgggatg cagccccacg tcgataccgc
      721 gctcgggaac gcattcaaac gcgcggagac gacaaggcag cccgcggcca ccgccgtcgt
      781 cacggcggcg cagaacgttc gccgggtcgt catcttcgat cccattgaaa gcaacgaaaa
      841 gctgctcggc tatctcgccg ccgagctcga tcccgccctg atatatcgaa acgtcataca
      901 acagcaggaa gtcgccgatt cgctgacgtc gggcaacatc ccgacgtatc acgcggtcta
      961 cggtcccaac cgcgcgctga tctctccacc actcgcggcg ccgcgcggat ggccgacgac
     1021 cacgcggccc attcgagtcg tcgatacgga atggctggtt gaagtcgcgt atcagcccgt
     1081 ggatccgcgt ttttatcgcg gccagcagat catgcgctgg gcgattggtt tgctgatcgc
     1141 ggtgttcacc gcactgtttt tcctgttcct gcgacggac
//
LOCUS       DASPLT010000141         1654 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_165828, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000141 DASPLT010000000
VERSION     DASPLT010000141.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1654)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1654)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1654
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_165828"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(38..451)
                     /locus_tag="VEA63_01480"
     CDS             complement(38..451)
                     /locus_tag="VEA63_01480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012377288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF983 domain-containing protein"
                     /protein_id="HYD82680.1"
                     /translation="MNVTRVQIAARGLTNRCPNCGGRTLFKGGTLFQVNKTCPTCGFV
                     IERDNDEGFFLGSLSLNYGFTLVGYLVPVMLLAYYDVVETTTAAILAGVGAVLVPALF
                     YRSSRSWWLMNYYLFLPHHLPANGGTGRAGEDNNR"
     gene            complement(666..>1654)
                     /locus_tag="VEA63_01485"
     CDS             complement(666..>1654)
                     /locus_tag="VEA63_01485"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HYD82681.1"
                     /translation="RLGVALVGLGYYSTDLLAPALQLTSKCHLAGVVSGTPSKIEAWQ
                     KRYAIPDANVYSYENFDAIANNPAIDVVYVVLPPSMHAEYTIRAAKAGKHVWCEKPMA
                     PSVRECEAMIRACKDNNVKLAIGYRLHHEPNTRTIMQFVRDQKYGKAVTATAEAGYRE
                     SRTDHWKQKKAMGGGVMGDMGVYPLNALRYGTGLEPIAVTAQASTTRPEIYSEVEETM
                     HFELEFPGGVTGVGDASFGKGMNKLRVNCAQGWYELAPFQSYNGVKGTTSDGVALDAT
                     IPNQQARQMDDDASAIMENTPLLAPGEEGLKDTIVVEAIYRSAAAGEKVSIG"
BASE COUNT          313 a          501 c          514 g          326 t
ORIGIN      
        1 tcgcccccaa atcgcgaaac cccgaacggc actacggtta gcgattgttg tcctcgccgg
       61 cgcggccggt gccgccgttg gcggggaggt gatgaggaag gaagaggtaa tagttcatca
      121 gccaccaact acgcgaggag cgatagaaga gcgcgggcac gagaacggcg ccgacgccgg
      181 cgagaatcgc ggcagtggtg gtctcgacga cgtcgtagta agcgagcagc atgacgggca
      241 cgaggtaacc gacgagggtg aagccgtagt tgagcgagag agagccgaga aaaaatccct
      301 cgtcgttgtc gcgttcgatg acgaagccgc aggtgggaca ggttttgttg acttgaaaaa
      361 gcgtgccgcc cttgaacagc gtgcggccgc cgcagttggg gcagcgattg gtgaggccgc
      421 gtgcggcgat ctggacacga gtgacgttca tggcgggagc agcgtagcga tcggagcggg
      481 tgaatctagg gagaaacggc gatgtgcgcg attcggaagg taggagacag gcctgcttgc
      541 aggcgatttc ggtgtgcaag cgagcaacat ggcctgcaag caggctccca cctccgatcc
      601 tgtaatcggc tactttaggt tgcacgcgtt cgtgcacggc gcgcctcacc ggtcggcgca
      661 cccgatcagc caatgcttac tttttctccg gcggcggccg atcgataaat ggcttcgacg
      721 acgatcgtgt ccttcaaacc ttcttcgcca ggcgctaaca acggcgtgtt ctccatgatc
      781 gccgaggcat cgtcgtccat ctgccgcgcc tgctgattgg gaatcgttgc gtcgagcgca
      841 acgccgtcgc tcgtggtgcc cttgactccg ttatacgact gaaacggcgc cagctcatac
      901 caaccttgcg cgcagttcac gcgcagtttg ttcatgcctt tgccgaagct cgcgtcgccg
      961 acgccggtga ctccgccggg aaattcgagt tcgaaatgca tcgtctcctc cacctcggag
     1021 tagatctccg ggcgcgtcgt cgacgcctgc gcggtgaccg cgatcggctc caaccctgtc
     1081 ccgtaacgaa gcgcattcaa cgggtagacg cccatgtcgc ccatgacgcc gccgcccatc
     1141 gctttctttt gcttccagtg atcggtgcgc gactcgcgat aaccggcttc cgccgtggcc
     1201 gtgacagcct tgccgtattt ctggtcgcgc acgaattgca tgatcgttcg cgtgttcggc
     1261 tcgtgatgca gccggtagcc gatcgcgagc ttcacgttgt tatctttgca cgcgcgaatc
     1321 atcgcctcgc actcgcgcac ggaaggagcc atcggctttt cgcaccagac atgtttgccg
     1381 gcctttgccg cgcggatcgt atactccgcg tgcatggacg gcggcaacac gacatagacg
     1441 acgtcgatcg ccgggttgtt ggcgatcgca tcgaaattct cgtagctgta aacattcgcg
     1501 tcggggatcg cataacgttt ttgccacgcc tcgatcttgc tcggcgtgcc tgaaaccacg
     1561 cccgccagat ggcacttcga cgtgagctgc aacgccggcg ccagcaagtc cgtgctgtag
     1621 taaccgagac cgaccaacgc gacgcccagc cgct
//
LOCUS       DASPLT010000142         3111 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_165846, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000142 DASPLT010000000
VERSION     DASPLT010000142.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 3111)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3111)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3111
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_165846"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(225..950)
                     /locus_tag="VEA63_01490"
     CDS             complement(225..950)
                     /locus_tag="VEA63_01490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD82682.1"
                     /translation="MNSAALSTIAVFGFTVAFFHALIPTHWLPFVLVARARKWSRVKT
                     LAVTAFAGLGHVGLTSLLGLAIAWFGFRLDEEVGEAFPWIAGGALIALGVYYGWRQIR
                     GKGICHHAVPGGHHHASEECGHESEDSHWEHEVKDSELVSGGRSDLAAIGGLFLMLTL
                     SPCEGFLPVYLSGVQYGWHGFVVLSAILALGALAGMLLVTWLALLGLERFKVRYFEHR
                     EAGALGALFCVLGVLVIALEHHH"
     gene            1148..2119
                     /gene="hemF"
                     /locus_tag="VEA63_01495"
     CDS             1148..2119
                     /gene="hemF"
                     /locus_tag="VEA63_01495"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007358868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HYD82683.1"
                     /translation="MPPDLPAVRSYLLALQDTICAALEQEDGVARFREDSWQRPENTG
                     GGRTRILGDGAVFEKAGVGFSHVRGAALPASATAHRPELAGRAWEALGVSLVLHPRNP
                     YAPTAHANVRFFHAAPQTSNPEPITQNPKLRTPNHGASAPVWWFGGGFDLTPYYGFEA
                     DAVHWHRTARDAVVPFGPALHPRFKKWCDDYFFLKHRNEPRGIGGLFFDDFNELGFDQ
                     SFALTRAVGDAFLPAYLPIVSRRKATPFTDRERQFQLYRRGRYVEFNLVWDRGTLFGL
                     QSGGRTESILMSLPPLVRWDYDWHPEPGTPEARLYDVFLKSRDWLTV"
     gene            2200..>3111
                     /locus_tag="VEA63_01500"
     CDS             2200..>3111
                     /locus_tag="VEA63_01500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HYD82684.1"
                     /translation="MNSRDRFLAACACQPLDLPPLWIMRQAGRYLPEYRALKAKSSFL
                     HMVQTPALAAEVTLQPLRRFALDAAILFSDILVIPEALGQPYHFRDAGGIEMEFRLES
                     RAQVDALAPASAIPERLAYVADTLALLKKELSGDKALLGFGGSPWTLATYMVEGGSSD
                     DFQRIKHLFYTDRATFDALLEKLTAALVTYFQLQIRAGADAIQIFDSWGGIVAGTDYE
                     AASLQWIRRIVAALPPNFPIILYAKNTAPHHLAQAASGIRVLSVDWTANLATLAQQLG
                     NRNSEPGTGGAPVALQGNLDPVLLNTTP"
BASE COUNT          594 a         1242 c          736 g          539 t
ORIGIN      
        1 ggtctctttc catttcccat gcgccggtcc ccgctggcga aaaacccgcc ctcgacgccg
       61 cggccgccga cgccccgccc gtcatcaagt tttcgattcc caacaaaaaa taacgcgcgc
      121 cgacgttagc ccacgccacc ccgcgcctac tcttctccgc ggagggacga cctccgtgtc
      181 gtccgttttc tcaccgctcc gctacccgct gtcgtgccct cgcatcaatg atgatgctcg
      241 agcgcgatca ccagcacacc gagcacgcag aacagcgccc ccagcgcccc cgcctcgcgg
      301 tgctcgaaat aacgcacctt gaaccgctcg agccccagca gcgcgagcca cgtcacgagc
      361 aacatccccg cgagcgcgcc gagcgccaga atcgcgctca acaccacgaa gccgtgccag
      421 ccgtattgca cgccggatag ataaacaggc aaaaaccctt cgcacggcga cagcgtcagc
      481 atcagaaaaa gccctccgat cgccgcgaga tcgctccgcc cgcccgacac cagctcgctg
      541 tccttcacct cgtgttccca atggctgtct tcgctctcgt gaccgcactc ctcgctcgcg
      601 tgatgatggc cgccgggcac cgcgtgatga cagatcccct tcccgcgaat ctgcctccac
      661 ccatagtaaa cgcccaacgc gatcaacgct ccgcccgcaa tccacggaaa cgcctcaccc
      721 acttcctcat cgagccgaaa cccaaaccac gcgatcgcca gcccgagcag actcgtgagc
      781 ccgacatgcc ccagccccgc gaacgctgtg accgcaagcg ttttcacccg cgaccacttc
      841 cgcgcccgcg ccaccagcac gaacggcaac cagtgcgtag gaatcaacgc gtgaaaaaac
      901 gccacggtga aaccgaaaac agcgatcgtg ctcaaagcgg cggaattcat ggattttcgg
      961 aaatgtcgat gaagccagcg agcgcgccag cctgtcgaac gcgaactcta tcaaagtcgt
     1021 caaccgccct gcctctccac ccaaaatcgt tctcgttctc gttctctttc tcgttctcct
     1081 tccgaaaccg agagaacgcc tacaagaaag agaacgagaa cgactaccgc cgacccgcct
     1141 cagcgccatg ccgcccgacc ttcccgccgt ccgcagttac ctcctcgcgc tgcaagacac
     1201 gatctgcgcc gcgctcgagc aggaagacgg cgtcgcgcgc ttccgcgaag attcctggca
     1261 acgcccggaa aacaccggcg gcggccgcac ccgcatcctc ggcgatggcg ccgtgttcga
     1321 aaaagccggc gtcggttttt cccacgtgcg cggcgctgcg cttcccgcgt ccgccaccgc
     1381 gcaccgcccc gaactcgccg gccgcgcctg ggaagccctc ggcgtctccc tcgtccttca
     1441 tccgcgtaat ccttacgccc ccaccgcaca cgccaacgtc cgcttcttcc acgccgcccc
     1501 gcaaacttca aaccccgagc ccataactca aaacccaaaa ctccgaactc caaaccacgg
     1561 cgcgtccgcg ccagtctggt ggtttggcgg cggcttcgat ctcacgccct attacgggtt
     1621 cgaagcagac gccgttcact ggcatcgcac cgcccgcgac gccgttgttc ctttcggtcc
     1681 cgcgctccat ccgcgtttca aaaagtggtg cgacgactac ttcttcctaa agcaccgcaa
     1741 cgaaccgcgc ggcatcggcg gacttttctt cgacgatttc aacgagctcg gtttcgatca
     1801 gtctttcgct ctcacccgcg ccgtcggcga cgcgtttctt cctgcgtatc ttcccatcgt
     1861 gtcgcgccgc aaagccacgc cgttcaccga ccgcgagcgc cagttccaac tctatcgtcg
     1921 cggccgatac gtcgagttca acctcgtctg ggatcgcggc acgctcttcg gcctgcaatc
     1981 cggcggccgc accgaatcga tcctcatgag cctcccgccg ctcgtccgct gggactacga
     2041 ctggcatccc gaacccggca cccccgaagc ccgcctctac gacgtcttcc taaaatcccg
     2101 cgactggctc acggtttaag gccatgcgtt cgcatccacg cgggtgacgc gaacaaggcc
     2161 tttcccattc gtgtgaattc gtgtccattc gtggttcaaa tgaatagccg cgatcgcttc
     2221 ctcgccgcct gcgcctgcca accactcgac ctccctccgc tctggatcat gcgccaagcc
     2281 ggtcgctacc tccccgaata ccgcgcgctc aaagcgaagt cctccttcct ccacatggtg
     2341 caaacccccg cgctcgccgc cgaggtcacc ttgcaacccc tgcgccgctt cgccctcgat
     2401 gccgcgattc tcttttccga catcctcgtc attccggaag cgctcggcca accttaccat
     2461 ttccgcgacg ccggcggcat cgaaatggaa ttccgcctcg agtcgcgcgc ccaagtcgac
     2521 gcgctcgctc ccgcttccgc cattcccgaa cgcctcgcct acgtcgccga cacgctcgcg
     2581 cttctcaaaa aagaactctc cggcgacaaa gcccttctcg gcttcggcgg ttccccctgg
     2641 accctcgcca cctacatggt cgaaggcggc agctccgatg actttcagcg catcaagcac
     2701 ctcttctaca ccgatcgcgc gaccttcgac gccctcctcg aaaaactcac cgccgcgctc
     2761 gtaacctatt ttcaactaca aatccgcgcc ggcgccgacg ccattcagat tttcgattcc
     2821 tggggcggca tcgtcgccgg caccgattac gaagccgcgt cgctccaatg gatccgccgc
     2881 atcgtcgccg cgctcccgcc gaatttcccg atcatcctct acgcaaaaaa cacggcgccg
     2941 catcacctcg cccaagccgc ctccggcatc cgcgtcctga gcgtcgactg gactgccaac
     3001 ctcgccacgc tcgcccagca actcggaaat cggaactccg aacccggaac cggcggcgca
     3061 cccgtcgcgc tgcaaggcaa cctcgatccc gtcctgctca acaccacgcc c
//
LOCUS       DASPLT010000143         2093 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_186074, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000143 DASPLT010000000
VERSION     DASPLT010000143.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 2093)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2093)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2093
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_186074"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..329)
                     /locus_tag="VEA63_01505"
     CDS             complement(<1..329)
                     /locus_tag="VEA63_01505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD82685.1"
                     /translation="MLGARWAAVDRAGAELPELDQWDAEGLYLLLPWFTDQPLLPALF
                     AAHNEHYVVLTKLLALGLTLANGQWDQRLECVAHAFIPASVAVAFLLWTRRRFTVGFT
                     ACTGLFFA"
     gene            complement(458..1180)
                     /locus_tag="VEA63_01510"
     CDS             complement(458..1180)
                     /locus_tag="VEA63_01510"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HYD82686.1"
                     /translation="MPKSLFDLKVNIYADGADKAGILDLYAKPYIKGLTTNPTLMRKA
                     GIKDYELFARDILETVTAKPLSLEVFSDEFPEMRRQALKIASWADNVYVKIPITNSRG
                     ESSIPLVRDLAHAGVKLNVTALLTVAQAASVAEALNPDIPSVVSLFAGRIADAGVDPQ
                     PIMRESLRLLTHHPKAELLWASVREVYNLLQAEQCGCHIVTVTHDILAKAAKMLGMDL
                     TALSLDTVKMFARDAAEAGYKL"
     gene            complement(1197..1742)
                     /locus_tag="VEA63_01515"
     CDS             complement(1197..1742)
                     /locus_tag="VEA63_01515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIIA family hydrolase"
                     /protein_id="HYD82687.1"
                     /translation="MPLRPAVFLDRDGTLNEQVIRDGKPYPPASLDEFKLFPDVAAAC
                     HSLKRAGYALIVVTNQPDVGRGTQSQLVIDAMHDRLRALIPEIDRVEVCYDPGRGEPS
                     RRRKPEPGMILDAAAALELDLAHSWLIGDRWRDVDCGKRAGVRTIFIDFHYAEQLREQ
                     PDHVVSSFAEAAEIILAHRLP"
     gene            complement(1820..>2093)
                     /locus_tag="VEA63_01520"
     CDS             complement(1820..>2093)
                     /locus_tag="VEA63_01520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sugar isomerase"
                     /protein_id="HYD82688.1"
                     /translation="GGNLAQNVSPNLVSALQLAKEVGAKIIGIVGKDGGFTATVADAC
                     VIVPTVNPNNITPHSEAFQAVIWHLFVSHPDLKVRQTKWESVTPTK"
BASE COUNT          405 a          545 c          750 g          393 t
ORIGIN      
        1 gcgaaaaaaa gaccggtgca cgcggtgaaa ccgacggtga acctgcgacg cgtccacaga
       61 agaaaagcga ccgcgaccga cgctggaatg aacgcatgcg cgacgcattc caaccgctgg
      121 tcccattgcc cgttcgcgag cgtaagtcca agcgcgagca gttttgtgag cacgacgtag
      181 tgctcattgt gcgccgcgaa gagggccggc aatagcggct ggtcggtgaa ccaaggaagc
      241 aggaggtaaa gaccctccgc gtcccattgg tccaattcgg ggagttcagc cccggcgcga
      301 tccaccgcgg cccatcgcgc tccgagcacg aagaggaaaa cgctcagggc gaggatcgcg
      361 aacgagtgct ttttcgcagc tccaaaccag cgcagtttcg ttagcttcga ggcgctggga
      421 ttgggcgttt caacgcaggc atgcgttgct tggacgttca caatttgtag ccggcctcag
      481 ccgcgtcgcg ggcgaacatc tttactgtgt cgagcgagag cgcggtgagg tccatgccga
      541 gcattttggc ggctttggcc aggatgtcgt gcgtcacggt gacgatgtgg cagccgcatt
      601 gctcggcttg gagcaggttg tagacttcgc ggacggaggc ccacaggagc tcggctttgg
      661 ggtggtgagt gagcaggcgc agcgattcgc gcatgatcgg ttggggatcg acgccggcgt
      721 ccgcgatgcg accggcgaag agcgaaacca cggacggaat gtcgggattg agtgcctcgg
      781 cgaccgacgc ggcttgagcg acggtgagga gcgcggtgac gttgagtttc acgcccgcgt
      841 gggcgagatc gcggacgagc gggatggagg attcgccgcg cgagttggtg atcgggatct
      901 ttacgtaaac gttatccgcc caactcgcga tcttcagcgc ctggcggcgc atctccggga
      961 attcgtcgga gaacacttcg agcgagagcg gtttggcggt gaccgtttcg aggatgtcgc
     1021 gcgcgaagag ttcgtagtcc ttgatgccgg cctttcgcat caaggtggga ttcgtggtca
     1081 ggcctttgat atacggcttg gcgtagagat cgagtatgcc ggccttgtcg gcgccatcgg
     1141 cgtagatgtt gaccttcaga tcgaaaagcg acttcggcat tgggtggaaa gagggtttat
     1201 ggcaagcgat gggcaagaat aatctcggct gcttcggcga agctggatac gacgtgatcg
     1261 ggctgctcgc gcaattgttc cgcgtaatga aaatcgatga agatcgtgcg aacaccggcg
     1321 cgtttcccgc aatcgacgtc gcgccagcga tcgccgatca gccatgagtg cgcgaggtcg
     1381 agctcgagcg cggcggcggc gtcgagaatc atgccaggct cgggcttgcg gcggcgcgaa
     1441 ggttcgccgc gaccgggatc gtagcagacc tcgacgcgat cgatttccgg gatgagcgcg
     1501 cgcaagcgat cgtgcatggc atcgatgacg agttgcgact gcgtgccgcg gcccacgtcg
     1561 ggctggttgg tgacaacgat caaggcgtaa ccggctcgct tgagagaatg gcaggcggcg
     1621 gcgacgtcgg ggaagagttt gaactcgtcg agcgaagcgg gcggataagg tttcccgtcg
     1681 cgaatgactt gctcgttcag cgtgccgtcg cgatcgagaa aaacggcagg acgcagcggc
     1741 atgtgggtgg gtgttgagag tgcggacaac gtcgcgatgc gctgagaggg gcagcgtggg
     1801 aaggcgggtt gaaacccgcc tacttggttg gggtgacgct ttcccacttg gtttgccgga
     1861 ccttcagatc gggatgcgaa acgaagaggt gccagatgac ggcctgaaac gcctcggagt
     1921 gaggcgtgat gttgttggga ttcacggtgg gcacgatgac gcaggcgtcc gcgacggtgg
     1981 cggtgaagcc gccgtctttt ccgacgatgc cgatgatttt ggcgccgacc tccttcgcga
     2041 gctgaagggc ggacacgaga ttcggggaga cgttttgcgc gaggttgccg cca
//
LOCUS       DASPLT010000144         1737 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_63227, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000144 DASPLT010000000
VERSION     DASPLT010000144.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1737)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1737)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1737
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_63227"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(49..>1737)
                     /locus_tag="VEA63_01525"
     CDS             complement(49..>1737)
                     /locus_tag="VEA63_01525"
                     /inference="COORDINATES: protein motif:HMM:NF012551.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0016052 - carbohydrate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding protein"
                     /protein_id="HYD82689.1"
                     /translation="DRYKNVVNDWDVINEAIDISAPNGLRNSPWLQIIGPDYIDLAFQ
                     FADEVLEGAGGLYINDFNTHEPAKRDALATVVQGLLNRGVRVDGVGHQTHIRIGWPSL
                     TEIAQSLDKFTAMGLDNQITELDISAYASDTDTSPVSAETLVLQGYRYRDLFNLFRAK
                     SSQISSVVFWGMADDNTWLKTFPIARDDKPLLFDEQLQAKPAYYGVIDPSQLPVLPAD
                     LNVTQKPSKGIVNNLESWVTVAPVALNPGDGSASWGRFRMLWSARDLHLAIEVDDATR
                     LRNGDRIEIFLPGGKRYTFSKLGLQRPSGAEGLILPTGRGYVLFARVPVSPAIAVGGE
                     LLFDVRVTNGANGAQQSWSDTHHAQDVEETGFGTLHFLPEKLVVPVDRGRATIDGQQD
                     KAWKNATEISTKRFALGTAGATAKVKLLWDSGYLYVYATVSDPLLSKASANPWEEDSA
                     EIFVDPNNAQTTAYQPGDAQYRINFDNEVSVGGSTSASNIVSATRRVSGGYVVEAAIR
                     IDPSETRRGSILGFDFQVNDDSTGSGARTSVATWNDASGNAYQDTSQFGAIILK"
BASE COUNT          307 a          484 c          599 g          347 t
ORIGIN      
        1 ggctgaggct ggcaggcacg gaggcctgcc gctacgcgga ggttgaggct atttcaggat
       61 gatggcgccg aactgggacg tatcctgata agcattaccg gaggcatcgt tccatgtcgc
      121 gacactcgtg cgagcgcccg agccggtcga atcatcgttc acttggaaat cgaagccgag
      181 gatcgagccg cgcctcgtct cgctcggatc gatgcggatc gcggcttcga cgacgtaacc
      241 gccgctcacc cgacgcgtgg cgctcacgat gttcgaggcg gaggtcgagc cgccgacgct
      301 gacttcgttg tcgaagttga tgcggtattg cgcgtcgccg ggttgatacg cggtggtctg
      361 agcgttgttg gggtcgacga agatttcggc ggagtcttcc tcccagggat tcgcgctggc
      421 tttgctcaac aacggatcgg acaccgtggc gtaaacgtag aggtaaccgg agtcccagag
      481 cagtttgacc ttcgcggtcg cgccggcggt gccgagagcg aaacgcttcg tcgagatctc
      541 ggtcgcgttc ttccacgctt tgtcctgctg gccatcgatc gtcgcgcgac cgcgatcgac
      601 cgggacgacg agtttttccg gcaagaagtg cagcgtgccg aaaccggtct cctcgacgtc
      661 ctgcgcgtga tgcgtgtcgc tccacgattg ttgcgcaccg ttggcgccgt tggtcacgcg
      721 gacgtcgaaa aggagttcgc cgccgaccgc gatcgcgggc gagacgggca ctcgggcaaa
      781 cagcacgtag ccgcggccgg tcggaaggat cagcccctcg gcgcccgaag gccgttgcag
      841 gccgagcttg gaaaacgtgt agcgtttgcc gccgggcagg aagatttcga tacggtcgcc
      901 gttgcggaga cgcgtggcat cgtcgacctc gatggcgaga tgaagatcgc gcgcgctcca
      961 caacattctg aaccggcccc aggacgcgga gccgtcgccg ggattgagtg cgacgggcgc
     1021 gacggtgacc caggactcga gattgttgac gatacctttg ctcggtttct gcgtgacgtt
     1081 gagatccgcg ggcaagaccg gcagttgcga gggatcgatc acgccgtagt aagcgggctt
     1141 cgcctggagc tgttcgtcaa agagcaacgg tttgtcgtcg cgcgcgatcg ggaaggtttt
     1201 caaccacgtg ttgtcgtcgg ccatgcccca gaaaacgacg gagctgatct ggctgctctt
     1261 cgcgcggaag aggttgaaca gatcgcgata gcggtagcct tgaaggacga gcgtttcggc
     1321 ggagacggga gacgtgtcgg tgtcgctcgc gtaagcgctg atgtcgagtt cggtgatctg
     1381 gttgtcgagg cccatcgccg tgaacttatc gaggctttgc gcgatttcgg tgagcgatgg
     1441 ccagccgatg cggatgtgag tctggtgtcc gacaccgtcg acgcggacgc cgcgattcag
     1501 gagaccttgc acgacggtcg cgagcgcgtc gcgcttcgcc ggttcgtgtg tattgaaatc
     1561 attgatatac aggccgccgg cgccctcgag gacctcgtcg gcgaattgaa acgcgaggtc
     1621 gatgtagtcg ggaccgatga tctgaagcca cgggctgttg cgcaggccgt tgggggcgga
     1681 gatgtcgatg gcctcgttga taacatccca gtcgttgacg acgtttttgt aacgatc
//
LOCUS       DASPLT010000145         2235 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_2193, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000145 DASPLT010000000
VERSION     DASPLT010000145.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 2235)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2235)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2235
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2193"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            199..876
                     /locus_tag="VEA63_01530"
     CDS             199..876
                     /locus_tag="VEA63_01530"
                     /inference="COORDINATES: protein motif:HMM:NF024863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HYD82690.1"
                     /translation="MNLRSLLRLAFVATTLAFVSSALAGPERWAANIDKFTQADATNP
                     PPRNGIVFIGSSSIVGWKSLAKDFPGLPTINRGFGGSQLEDSVFYADRIVIPYAPRTV
                     VLYAGDNDIAAGKTPETVLADFRAFREKIHAALPDTRVLYLSIKLSPSRAKFKDAAVR
                     TNELIAADCAAAKNCTFVDVNAPLLDHEGNPRPIFFRKDMLHLLPWSYDLWTEVLAPH
                     LVDKAKS"
     gene            873..1475
                     /locus_tag="VEA63_01535"
     CDS             873..1475
                     /locus_tag="VEA63_01535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009512553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HYD82691.1"
                     /translation="MSDAVGRLVRQIQFIAECDKLKEIFRQTINTQSRRAENDAEHSW
                     HLCLCVIVLAEHANVPTLDVLRVLKMVILHDLVEIDAGDTFAYDVAAMVGQHEREAIA
                     ADRIFGLLPADQAQDFRALWDEFEEKKTPEAKFAAAVDRFQPMLLNCLTQGDAWARHG
                     VTHDRVVARNCHIADGSATLWRHAEQLLNEVVAAGHLRKI"
     gene            complement(1612..>2235)
                     /locus_tag="VEA63_01540"
     CDS             complement(1612..>2235)
                     /locus_tag="VEA63_01540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyguanosinetriphosphate triphosphohydrolase"
                     /protein_id="HYD82692.1"
                     /translation="CQIMDWADDAAYSLNDIVDGVKAGFLTQEKIENWAAGEAIDARR
                     QRWLDDVFDAIRSDRLEGVFSQKIGAFITACRLRERDNFMAEKTNRYRFELIVSPEAQ
                     DEALFYKKMANDIILESPQLQQIEHKARRVLFDLWESAWRNYVEKGARVINILPTRVG
                     KLVDAQTTKAGKARQICDYLAGLTDGGIVRTYRRLFDPEFGSIRDLS"
BASE COUNT          448 a          824 c          562 g          401 t
ORIGIN      
        1 ggactttcat gggacaaaaa tgcttccgaa ggaaatcccc cgcgaagcct ctagcaagga
       61 aaaggtttca caccgaaagc ggtgggagga gctttacgcc ccaaccagcg ccacgccgtt
      121 ctaacttcgc ggcataaagc ccgccccatc cgcgttcaaa ccgcttgccc accccgccgc
      181 ctcgcctttc gctcgcgtat gaacctgcga tctctcctcc gcctcgcctt cgtcgcaacc
      241 acgctcgctt tcgtcagcag cgctctcgcc ggccccgaac gctgggccgc caacatcgac
      301 aagttcaccc aagccgacgc cacgaatcct ccgccgcgca acggcatcgt cttcatcggc
      361 agttcctcaa tcgtcggctg gaaatctctc gccaaggact ttcccggctt gcccacgatc
      421 aaccgcggct tcggcggttc gcagctcgaa gacagcgtgt tctacgcgga tcgcatcgtg
      481 attccctacg cgcctcgcac cgtcgtcctc tacgccggcg acaacgacat tgccgccggc
      541 aagacccccg aaaccgtcct cgccgacttc cgcgccttcc gcgaaaaaat ccacgccgcg
      601 ctgcccgaca cgcgcgtcct ctatctctcg atcaagctca gcccgtcgcg cgcgaaattc
      661 aaggacgccg ccgtccgcac caacgagctc atcgccgccg actgcgcggc cgcgaaaaac
      721 tgcaccttcg tcgacgtcaa cgctccgctc ctcgaccacg agggcaaccc gcggccgatt
      781 tttttccgca aggacatgct gcacctgttg ccctggtcct acgacctctg gaccgaagtg
      841 ctcgcgcccc atctggtcga caaagcgaaa tcatgagcga cgccgtcggc cgtctcgtcc
      901 ggcaaattca gttcatcgcc gagtgcgaca agctgaagga aatcttccgc cagacgatca
      961 acacgcaaag ccgccgcgcc gaaaacgacg ccgagcattc ctggcatctc tgcctctgcg
     1021 tcatcgtcct cgccgagcac gccaacgttc ccacgctcga tgttctgcgc gtgttgaaaa
     1081 tggtgatcct gcacgacctc gtcgaaatcg acgccggcga caccttcgcc tacgacgtcg
     1141 ccgcgatggt cggccagcac gagcgcgaag ccatcgccgc cgatcgcatc ttcggacttc
     1201 ttcccgccga ccaagcgcag gatttccgcg cgctctggga cgaattcgag gaaaagaaaa
     1261 cgcccgaggc taaatttgcc gccgccgtcg atcgctttca gccgatgctg ctcaattgcc
     1321 tcacgcaagg cgacgcatgg gcccgccacg gcgtgacgca cgaccgcgtg gtcgcgcgca
     1381 actgccacat cgccgacggc agcgccactc tctggcgcca cgccgagcag ttgctcaacg
     1441 aggtcgtcgc cgccggccat ctccgcaaaa tctgaacgaa acagcgctcc tcaaatccgg
     1501 acacccgccc gcgcctcgcc gatcaggcga ggcacgcacc gccacctacg ccagaaacct
     1561 tccgaaagat cagcgcagat tagcggttaa aaacaaaaag agtcatccgc ctcagctcag
     1621 atcccggatc gatccaaact ccgggtcgaa caaccgccga tacgtccgca cgattccgcc
     1681 gtcggtcagc ccggcgagat aatcgcagat ttgccgcgcc tttcccgcct tcgtggtttg
     1741 cgcatcgacg agcttcccga cgcgcgtcgg caggatgttg atcacgcgcg cacctttctc
     1801 gacgtagttg cgccacgccg actcccataa atcgaacaac acccgccgcg ccttgtgctc
     1861 gatttgctgc aattgcgggc tctcgagaat aatgtcgttc gccatcttct tataaaacag
     1921 cgcctcgtcc tgcgcctccg gcgagacgat caattcgaag cgatagcgat tcgttttttc
     1981 cgccatgaaa ttgtcgcgct cgcgcagtcg gcacgccgtg atgaacgccc cgatcttctg
     2041 ggagaaaacg ccctccaatc gatcggagcg aatcgcgtcg aacacgtcgt ccagccagcg
     2101 ctgccgccgc gcatcgatcg cttcgccggc cgcccagttc tcgatttttt cctgcgtgag
     2161 aaaccccgcc ttcacgccgt cgacgatgtc gttcaacgaa tacgcggcat cgtccgccca
     2221 atccatgatc tggca
//
LOCUS       DASPLT010000146         2336 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_289424, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000146 DASPLT010000000
VERSION     DASPLT010000146.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 2336)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2336)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2336
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_289424"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..604
                     /locus_tag="VEA63_01545"
     CDS             <1..604
                     /locus_tag="VEA63_01545"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HYD82693.1"
                     /translation="ELDRVVRTYDMKGILLYTNLAGKFSDEPEFRPLFARAVELDIPI
                     LLHPAKPITTEFVKAYELTSTLGNMFDNTIAITRLLMSGILDEFPKLKLVCPHLGGTL
                     PYIVGRLDHQVSVLKRGPRNLTRLPSEYLKQIWMDVVSPLPLAVKFGHDFIGPDRLLY
                     SSDHPWVEPQLIVDTVQAAGLPRESEQKLFSTNARKLFRL"
     gene            601..1626
                     /locus_tag="VEA63_01550"
     CDS             601..1626
                     /locus_tag="VEA63_01550"
                     /inference="COORDINATES: protein motif:HMM:NF025465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PmoA family protein"
                     /protein_id="HYD82694.1"
                     /translation="MSRLPSSVRAAFQGIALSALSFCSFACTTSTTTPAPSPDRVVLT
                     PLSDRVRVEIDGYLFTEYRFRDGPKPCLYPIQDQEGTFYTRHWPMKKVDGEIHDHDWH
                     RSVWFAHGLVNGHDFWRELADNSTGRIVHQEILSTRSGAAGELQVRNHWIAASNEIIC
                     SDTTRYRFERVAEGYFLDCEVTLQATHGEVVLGDTEEGALGVRINEGIRLMVGKGKNR
                     TRGTGKIINAQGDTDRAAWGKRAPWCDYNGTVDGKHIGIAIFDHPKNPSHPTWWHVRD
                     YGLFAANPFGKHDFEKLKDQPNAGDITIPAGGEFTLRYRIFFHQGDEQSARVAQHYAD
                     YAADAAH"
     gene            1649..>2336
                     /locus_tag="VEA63_01555"
     CDS             1649..>2336
                     /locus_tag="VEA63_01555"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HYD82695.1"
                     /translation="MQRRAILFFLFATICTTAALATERPPNVVLILADDLGLNPVGAY
                     GNPYAYTPVLDRLAREGLVFTAAYAASPVCSPTRTAIMTGKSPARTRLTDFVPGHPFP
                     YARLRQPTWRKSINEKILPQFLAERGYVTGVFGKWHVPNRYTAPQGDSNGSKRHGFHE
                     AFITQKPADDSDAEADAHHLDAITERALDFIERHQHEPFFLHVSTHTIHSPLLAPEAL
                     VAKHRQRPGAG"
BASE COUNT          448 a          873 c          599 g          416 t
ORIGIN      
        1 ggagctcgat cgtgtcgttc gcacctacga catgaagggc attctcctct acacaaacct
       61 cgccggaaaa ttctccgacg agccggagtt ccggccgctg tttgcccgcg ccgtggaact
      121 cgacatcccg attctcctcc acccggcgaa acccatcacg accgagttcg tgaaggccta
      181 cgagctcacc agcacgctcg gcaacatgtt cgacaacacg atcgcgatca cccgcctgtt
      241 gatgtccggc atcctcgacg agtttccaaa gctgaaactc gtctgcccgc atctcggcgg
      301 cacgctcccc tacatcgtcg gccgtctcga ccaccaggtc agcgtcctca agcgcggccc
      361 gcgcaatctc acgcgcctgc ccagcgaata cctgaaacag atctggatgg acgtcgtctc
      421 gccgctccct ctcgccgtca agttcggcca cgacttcatc ggaccggacc gcctcctcta
      481 ctccagcgac catccctggg tcgaaccgca gttgatcgtc gacaccgttc aggccgccgg
      541 cctcccgcgc gagtccgagc aaaaactttt ctccaccaac gcccgcaaac tcttccgtct
      601 atgagccggc tcccttcgtc ggttcgcgcc gccttccaag gcatcgcgct cagcgcgttg
      661 agtttctgca gcttcgcctg caccacctcc acaacgaccc cagcgccatc gcccgaccgc
      721 gtcgtgctca cgcccctgag cgatcgcgtg cgcgtcgaga tcgatgggta tttgtttacc
      781 gaataccgct tccgcgacgg tccgaaaccg tgcctctatc cgatccagga tcaggagggc
      841 actttctaca cgcgccactg gccgatgaag aaggtcgacg gcgagattca cgaccacgac
      901 tggcatcgct cggtctggtt cgcccacgga ctcgtcaacg gccacgactt ctggcgtgaa
      961 ctcgccgaca actccaccgg ccgcatcgtg catcaggaga ttctcagcac ccgcagcggc
     1021 gccgccggcg agctgcaggt ccgcaatcac tggatcgccg cgtccaacga aatcatctgc
     1081 tcggacacca cgcgctatcg cttcgaacgc gtcgccgaag gctatttcct cgattgcgag
     1141 gtcactttgc aggcgacgca tggcgaggtc gtcctcggcg acactgagga aggcgcgctc
     1201 ggcgtgcgca tcaacgaggg catccgtctc atggtcggca agggcaagaa ccgcacgcgc
     1261 ggcaccggca aaatcatcaa cgcccaaggc gacaccgatc gcgccgcgtg gggcaagcgc
     1321 gcgccgtggt gtgactacaa tggcaccgtc gacggcaaac acatcggcat cgcgattttc
     1381 gatcacccga aaaatccgtc gcacccgacg tggtggcacg tccgcgacta cggcctcttc
     1441 gccgccaatc ctttcggtaa acacgatttc gaaaaactga aggatcaacc caacgccggc
     1501 gacatcacca ttcccgctgg cggcgagttc acgttgcgct accgcatttt ctttcaccaa
     1561 ggcgatgaac aaagcgcgcg cgtagcccag cactacgcgg actacgcggc cgacgccgct
     1621 cactaatcct gcggtgcttg tttttccgat gcagcgtcgc gcgattcttt tcttcctttt
     1681 cgcgaccatc tgcaccacgg ccgccctcgc gaccgagcgc ccgcccaacg tcgtgttaat
     1741 tctcgccgac gacctcggcc tcaatcccgt cggcgcctac ggcaaccctt atgcttacac
     1801 gcccgtcctc gaccggctcg ctcgcgaggg cctcgtgttc acggccgcct atgccgcgtc
     1861 gcccgtttgc tcaccgacgc gcaccgcgat catgaccgga aaatcccccg cgcgcacgcg
     1921 tctcaccgat ttcgttcccg gccatccgtt cccctacgcg cggctgcggc agcccacttg
     1981 gagaaaatcc atcaacgaaa agattctccc acagttcctc gccgagcgcg gttacgtcac
     2041 cggcgttttc gggaaatggc atgtccccaa ccgctacacc gcgccgcaag gcgacagcaa
     2101 cggttccaag cgccacggct tccacgaagc gttcatcacg caaaaaccgg cggacgattc
     2161 cgacgccgaa gccgacgctc accacctcga cgccatcacg gaacgcgccc tggacttcat
     2221 cgaacgtcat caacacgagc cgttctttct ccacgtttca acccacacga tccactcgcc
     2281 gctcctggcc ccggaggctc tagtcgcgaa acaccgccag cgccccggcg ccggcg
//
LOCUS       DASPLT010000147         1352 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_23525, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000147 DASPLT010000000
VERSION     DASPLT010000147.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1352)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1352)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1352
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_23525"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1235)
                     /locus_tag="VEA63_01560"
     CDS             complement(<1..1235)
                     /locus_tag="VEA63_01560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HYD82696.1"
                     /translation="MITSIRTVCVLLIGLLGLSACSRQEPASTAVAARPTIIEATNRY
                     EAVPIGAAVEGKPWISHVAIVDLDRDGRPDVLACDDKALAVVWIRQVTPGKYEESTVV
                     AELPAPVHAEAVDFDGDGDLDILVACMGEVFPNNDKIGSVVVLENDGQQRFTKRVLLD
                     QVARVTDVRAGDFNRDGKLDLAVAQFGYDQGEIRWMENLGGWKFRSHNLLNLSGTINV
                     CVADMNGDGALDITALVSQQWEEIHLFENDGRGGFTNKIIFGSTNEDYGSSGITLCDL
                     NRDGRPDVLYTNGDGFAYADPGTRPWHGVQWLENRGDGFFKFHRIGNLAGAYAPTGID
                     LDGDGDTDIVATSGFNDWTKPNAVSVVAFMNDGHENFAPHVLAYAPIQLIGCAAADMD
                     GSGTPSIVTGGFYSYPPFDR"
BASE COUNT          277 a          444 c          377 g          254 t
ORIGIN      
        1 cgatcgaacg gcggataaga ataaaatccg cccgtcacga tcgacggcgt gcccgatccg
       61 tccatgtcgg ccgccgcgca accgatcagt tgaatcggcg catatgcgag cacatgcggc
      121 gcgaagtttt cgtggccgtc attcatgaac gccacgacgg acacggcgtt cggcttcgtc
      181 caatcgttga aaccgctcgt ggccacgatg tcggtgtcac cgtcgccgtc gagatcgatg
      241 ccggtcggcg catacgcgcc cgcgagattc ccgatgcggt gaaacttgaa gaagccgtcg
      301 ccgcgatttt ccagccactg gacgccgtgc cacggacgcg tgccggggtc ggcgtaggca
      361 aatccgtcgc cgttcgtata gagcacgtcc ggccgaccat cgcggttgag atcgcacagc
      421 gtgattccac tgctgccgta atcctcgttc gtcgaaccga aaatgatctt gttcgtgaaa
      481 cctccgcggc cgtcgttttc gaacagatga atctcctccc actgctgcga caccagcgcc
      541 gtgatatcga gcgcgccgtc gccgttcatg tcggccacgc acacgttgat cgtcccggaa
      601 agatttagca gattgtgact gcgaaacttc cagccgccga gattctccat ccaacgaatc
      661 tcgccctgat cataaccaaa ctgcgccacc gcaagatcga gtttcccgtc gcggttgaaa
      721 tctcccgcgc ggacatcggt cacgcgtgcg acttgatcga gtaacacgcg cttcgtgaaa
      781 cgctgctgcc catcattctc cagcaccacg acggatccga tcttgtcgtt gttgggaaac
      841 acctcgccca tgcacgccac cagaatgtcg agatcgccat cgccatcgaa atccaccgcc
      901 tccgcatgca ccggcgcggg caattcggcc acaaccgtgc tctcttcgta tttccctggc
      961 gtaacctgcc ggatccaaac gacggcaagc gctttgtcgt cgcacgcgag cacgtccggt
     1021 cgcccgtcgc ggtcgagatc gacgatcgcc acatgcgaaa tccacggctt gccttcgacc
     1081 gccgcgccaa tcggcacggc ttcgtagcgg ttcgttgctt caattatcgt cggcctcgcg
     1141 gcgaccgccg tggatgcggg ctcctggcga ctgcaagccg aaagaccgag cagtccgatc
     1201 aggagaacgc acacggtgcg gatcgaagta atcatggctg cgatgaaatc tggagcgaag
     1261 cgtctacgaa atagtcgaag ccggcggcat ccccggaacg atcaaggagc atggccaaac
     1321 gctgcctgat actccgcgat cacccgggcg ag
//
LOCUS       DASPLT010000148         1417 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_23605, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000148 DASPLT010000000
VERSION     DASPLT010000148.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1417)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1417)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1417
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_23605"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..195)
                     /locus_tag="VEA63_01565"
     CDS             complement(<1..195)
                     /locus_tag="VEA63_01565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD82697.1"
                     /translation="MDTNGHEWGRRWITAVAGLVVGGLVVALLPERVLFVHDERVVQE
                     EVTGERWACPMMDFIGNKPGE"
     gene            complement(323..772)
                     /locus_tag="VEA63_01570"
     CDS             complement(323..772)
                     /locus_tag="VEA63_01570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD82698.1"
                     /translation="MIRHFITFLATFVVGAVVAFAARAALHQPHTEATPLAAAEPAPM
                     VDNALTPPKQKLAAGKASETKAKEAKGDEGHGEHAKAEAPKAEAEAKKPVNTVCAICG
                     MDVDPKLPTEEYKGQAIGFGCRMCPPKFRADPDKYGPYYLRNEVIQR"
     gene            complement(801..>1417)
                     /locus_tag="VEA63_01575"
     CDS             complement(801..>1417)
                     /locus_tag="VEA63_01575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD82699.1"
                     /translation="PGVGRPDFAAPTVEEIDPADAAALSLAAARVSEAEAMGKMARSS
                     GRPMTGVGLRFESERSGMGNAEALGVVFSSEIPFRSRRYARAEALAAEAEREAAEAEG
                     RGARYRIASAVSRVERAERLAERARSVARETRARLDAQYEALVRAAGTGSASGESTVL
                     MAVEILEQVTDTELKVIDADVAARVARAELWRYVPVHRFLSNKD"
BASE COUNT          236 a          580 c          327 g          274 t
ORIGIN      
        1 ctcgcccggc ttgttgccga taaaatccat catcggacac gcccaccgtt cccccgtcac
       61 ctcctcctgc accacccgct cgtcatgcac gaacaacacc cgctccggca acaacgccac
      121 cacgagcccg cccaccacca accccgccac cgcggtgatc caacgccgcc cccattcgtg
      181 tccattcgtg tccattcgtg gttaaaaaaa gttagtttcc cggtgcctag cgaacgcgac
      241 gcgccccaaa tccgtgtcca tccgtggtta aaaaatccgc ggttatccct ttcgtgtctt
      301 tcgaggttca ccccgcacac gctcaccgct gaatcacttc attccgcaga taatacggcc
      361 cgtatttatc cggatcggct ctgaacttcg gcggacacat ccgacacccg aaaccaatcg
      421 cctgcccttt gtattcctcc gtcggcaact tcggatccac atccattccg cagatcgcgc
      481 agaccgtgtt caccggcttc ttcgcctccg cctctgcctt cggcgcctcc gccttcgcgt
      541 gctcaccgtg accttcatcg cctttcgcct ccttcgcttt cgtctctgac gctttccccg
      601 ccgcgagttt ctgcttcggc ggcgtcaatg cattgtccac catcggcgcc ggctcggccg
      661 ccgccaacgg cgtcgcctca gtgtgcggtt gatgaagcgc cgcgcgcgcc gcaaacgcga
      721 cgaccgcccc cacgacgaac gtggcgagaa atgtaatgaa atgtcggatc atggtaggat
      781 tcgtttaatg gttgttgggt ttaatccttg ttggaaagaa accggtgcac cggaacataa
      841 cgccacaact ccgcgcgcgc gacccgcgcc gcgacatccg cgtcgatcac cttcagttcc
      901 gtgtccgtca cttgctcgag aatttcgacc gccatcaaca ccgtcgattc gccgctcgca
      961 ctgcccgtcc ccgccgcgcg caccagcgct tcgtattgcg catccagcct cgcccgcgtc
     1021 tctcgcgcca cgctccgcgc ccgctccgcc agccgctccg cacgctcaac gcgactcacc
     1081 gcgctcgcga tccgataacg cgcaccgcgc ccttcggcct ccgccgcttc ccgctccgct
     1141 tccgccgcca gcgcctccgc gcgcgcataa cgacgactgc gaaatggtat ctccgaactg
     1201 aatacaacgc ccaacgcttc cgcattgccc atccctgaac gttcgctctc gaaccgcagc
     1261 cccacgcccg tcatcggtcg cccgctcgag cgcgccattt tccccattgc ctccgcctcg
     1321 ctcacccgcg ccgccgccag gctcaacgcc gccgcgtccg ccggatcgat ctcctccacc
     1381 gtcggcgccg caaaatccgg ccgcccgacg cccggcg
//
LOCUS       DASPLT010000149         1043 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_207292, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000149 DASPLT010000000
VERSION     DASPLT010000149.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1043)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1043)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1043
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_207292"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..210
                     /locus_tag="VEA63_01580"
     CDS             <1..210
                     /locus_tag="VEA63_01580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYD82700.1"
                     /translation="YDAAYVLVDAIKRAGSTEGPAIRDALAATRNFAGVTGVTNINEN
                     RDASKPAAILEIKDGKLTFLKTVAP"
     gene            399..>1043
                     /locus_tag="VEA63_01585"
     CDS             399..>1043
                     /locus_tag="VEA63_01585"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HYD82701.1"
                     /translation="MTEFLQQLLNGLSLGAIYALIALGYTMVYGVLRFINFAHSDVFM
                     VGAFIGYYAGRIIPENTIWGGFTVLLLAMTGCALLGMRIERTAYRPLREPTEFNLVTI
                     LGALGAGALAIFLTRGLASLEFGLPLRIVICWASAEVAALLLARTGWKIVLQRTQGGA
                     PTLNVLITAIGVSLLLEYSGQLIFGAAPRTFPSVFPSMNFQLGKLTISSNQLFVI"
BASE COUNT          182 a          376 c          279 g          206 t
ORIGIN      
        1 tacgacgccg cctatgtgct cgtcgacgcc atcaagcgcg ccggctcgac cgaaggcccg
       61 gcgatccgcg acgcgctcgc cgcgacgaga aatttcgccg gcgtcacggg cgtgacgaac
      121 atcaacgaaa accgcgacgc ctcgaagccc gccgcgatcc tggagatcaa ggacggcaag
      181 ctcaccttcc tcaagaccgt cgccccctga gacgcaagag gcgagcgagc cagccaacaa
      241 atcgttctcg ttcctcgtcc tctttctcgt tctcgatcga tcctgaaaag agaacgagaa
      301 cgaagaacga gaaagagaac gatacgatcc gtttcgaccg tgagcccgcc gctcgtccac
      361 gcctcgacgt tcttatcccc gttctctgct agcaccccgt gaccgaattc cttcaacagc
      421 tcctgaacgg cctttcgctc ggcgccatct acgcgctcat cgcgctcggc tacacgatgg
      481 tctacggcgt gctgcgcttc attaacttcg cgcattccga cgtcttcatg gtcggcgcgt
      541 tcatcggcta ctacgccggc cgtatcatcc cggagaatac gatctggggt ggtttcaccg
      601 tgctgctcct tgcaatgacc ggttgcgcgc tgctcggcat gcgcatcgaa cgcacggcct
      661 accgcccttt gcgcgagccg accgagttca acctcgtgac catcctcggc gcgctcggcg
      721 ccggcgcgct cgcgatcttc ctcacccgcg gcctcgcctc gctcgagttc ggcctgccgc
      781 tgcgaatcgt gatctgttgg gcgagcgccg aggtcgccgc gctgctcctc gcgcgcaccg
      841 gctggaaaat cgtgcttcaa cgcacccagg gcggcgcgcc aaccctcaac gtcctgatca
      901 ccgcgatcgg cgtgtcgttg cttctcgaat acagcggcca gctcatcttc ggcgccgcgc
      961 cgcggacttt tccatcggtg tttccgtcga tgaatttcca gctcggcaag cttaccattt
     1021 ccagcaacca gctcttcgtc atc
//
LOCUS       DASPLT010000150         1569 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_227208, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000150 DASPLT010000000
VERSION     DASPLT010000150.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1569)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1569)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1569
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_227208"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..248
                     /locus_tag="VEA63_01590"
     CDS             <1..248
                     /locus_tag="VEA63_01590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HYD82702.1"
                     /translation="AVGATLTKGQTFGVVESVKAASDLYAPVSGTVLEANATLNNMPE
                     TLNREPYGEGWMLKIRLSNAGEAGSLLDAEGYAKSVE"
     gene            complement(546..>1569)
                     /locus_tag="VEA63_01595"
     CDS             complement(546..>1569)
                     /locus_tag="VEA63_01595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007363903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HYD82703.1"
                     /translation="VESASAGNIIGLSGFEDVDIGDTLDMRDDGHALPFTQIDPPTLE
                     MQFSVNDGPLVGQEGKLVTSRQIRERLMRELKLNVSIQIEDSDKAGVFNVKARGAMQV
                     AVLVETMRREGYELLVSRPTVIEKRGDNGERLEPYETVWIEVPDECVGSIMQNLANRK
                     GQLTNMEKLQHSTMIEATITTRGLIGLEIDVINATSGRGVTSHLFKEYGPYAGEVLTR
                     VTGTLIATEAGETTTYALLMVQERGKLFVGPGEKVYEGMIVGENPRNEDIAVNAVREK
                     KLTNFRSQGEGVAAGLTPPTKLSLERSIEYIAADELLEVTPKSLRLRKRILDNGARQK
                     AAKAAK"
BASE COUNT          260 a          455 c          538 g          316 t
ORIGIN      
        1 cggccgtcgg cgctacgctg accaaaggtc agacgtttgg cgtcgtcgaa tcggtgaaag
       61 cggcgagcga tctttatgcg ccggtgagcg gcaccgttct cgaagccaat gcgacgctca
      121 acaacatgcc ggagacgctg aaccgcgagc cttacggcga aggttggatg ctcaagattc
      181 gtctgagcaa cgcgggcgaa gcaggcagct tgctcgacgc ggaaggttac gcgaagtcgg
      241 tggagtgagc ggtcgccgca cggcgcggcg agcgtcgcaa gccgtagcgg caagccttcg
      301 tgcctgcctg ctcgccggtt cggaacattg tagggggtgc gctcgtcgca ccccgttgcg
      361 cacaagcgcg caacccacaa tccgagcagg caggcacgaa ggcctgccgc tacatgagaa
      421 gccggacgcg aaaaagcccg cgagcgtttt agcgctgcgc gggctttgaa gggaaggcgg
      481 cgcgttcgtg gcgctgctac aattcgcgag ggtggacgcg aggaccaccg ccacgatcgt
      541 gaaggttact ttgctgcttt cgcggctttt tggcgggcgc cgttgtcgag gatgcgcttg
      601 cggagtcgca gcgacttcgg cgtcacctcg aggagttcgt cggcggcgat gtattcgatc
      661 gagcgctcga gcgagagctt cgtcggcggc gtgaggccgg cggcgacgcc ttcaccttgc
      721 gagcggaagt tggtgagctt cttttcgcgc acggcgttga ccgcgatgtc ttcattgcgc
      781 ggattttcgc cgacgatcat gccttcgtaa actttttcgc cgggaccgac gaagagcttg
      841 ccgcgttcct gcaccatgag cagcgcgtag gtcgtcgttt cgcccgcttc cgtggcgatg
      901 agcgtgccgg tgacgcgcgt gaggacttcg ccggcgtagg ggccgtattc tttgaacaag
      961 tgcgacgtca cgccgcggcc gctggtggcg ttgatcacgt cgatttcgag gccgatcaaa
     1021 ccgcgcgtcg tgatcgtcgc ttcgatcatt gtcgagtgct gcagcttctc catgttggtg
     1081 agctggcctt tgcggttggc gaggttctgc atgatcgagc cgacgcactc gtcgggcact
     1141 tcgatccaaa cggtttcgta gggctcgaga cgttcgccgt tgtcgccgcg tttttcgatg
     1201 acggtcgggc gcgagacgag aagctcgtaa ccttcgcggc gcatcgtttc gacgagcacg
     1261 gcgacctgca ttgcgccgcg ggccttgacg ttgaacacgc cggccttgtc ggagtcctcg
     1321 atctgaatcg agacgttgag tttcaactcg cgcatcaagc gttcgcgaat ctgacgcgac
     1381 gtgacgagct tgccctcctg gccgacgagc gggccgtcgt tgacggagaa ctgcatctcg
     1441 agcgtcggcg ggtcgatctg cgtgaagggc agggcgtgac catcgtcgcg catgtcgagc
     1501 gtgtcgccga tgtcgacatc ctcgaagccg gagagaccga tgatgttgcc ggcgctggcg
     1561 gattcgacc
//
LOCUS       DASPLT010000151         1044 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_227221, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000151 DASPLT010000000
VERSION     DASPLT010000151.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1044)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1044)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1044
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_227221"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..462
                     /locus_tag="VEA63_01600"
     CDS             <1..462
                     /locus_tag="VEA63_01600"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HYD82704.1"
                     /translation="RAIIANRANADVFVSIHFNALPNDQRTRGTEVFTFAPARQRSTN
                     AWSLGANDDTEQAAAPANRHDAANAMLAHALHRELLEELKTFDRGKKIGHLGVLRGLN
                     CPGVLVESGFLSNDEEARLIATPAYRQRIAEALAEGIGDYAALVGSLQTKR"
     gene            743..>1044
                     /locus_tag="VEA63_01605"
     CDS             743..>1044
                     /locus_tag="VEA63_01605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012377334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD82705.1"
                     /translation="MSKRLSLLLVLVAAVSQPMLRAWDYAGHRIVNETALAALPEDFP
                     AFVRDAEAAERVAFLAGEPDRWRNVPDLPIKQANGLDHYLDIEHLIDAGMDVAKLPS"
BASE COUNT          191 a          277 c          377 g          199 t
ORIGIN      
        1 cgcgcgatca tcgccaaccg ggcgaatgcg gacgtgttcg tcagcattca tttcaatgcg
       61 ctgcccaacg atcagcggac gcgagggacg gaagtgttca cgtttgcgcc cgcgcggcag
      121 cggtcgacaa atgcgtggag cttgggcgcc aatgacgaca ccgagcaggc ggcggcgccg
      181 gcgaaccgcc atgacgcggc gaacgcgatg ctcgcgcatg cgttgcaccg cgagttgctg
      241 gaagaactga agaccttcga tcgtggaaaa aagatcggtc acctcggcgt gttgcgcggg
      301 ctgaattgtc cgggcgtgct ggtggaatcg ggatttttgt cgaacgacga ggaagcgcgg
      361 ttgatcgcga cgccggcgta tcggcagcgg atcgccgagg cgcttgcgga ggggatcggc
      421 gactacgcgg cgctggtggg aagtttgcag acgaagcgtt gaaagcgggt aggaaggcag
      481 gctcggtgtt gggggattaa gcacggatga acacggattc gatccgcgat cttgaaccac
      541 gaatggacac gaattcacac gaacttgcgc ggaaggtcgg cgagggttga aggtaggtgg
      601 cgcgcttgcg cgccccgttg cgggcaagcc cgcgacctac cgtcgaacag atgaggcttg
      661 ttgcttcgga tgctggtgtt gtccgggtga gttttcgaaa cgccgctttg ctccggcgcg
      721 gaaaactgat ttaccagcgg ccatgtcgaa acgcctttcg cttcttctcg tcctggttgc
      781 ggcggtgtcg cagccgatgc tgcgtgcttg ggattatgcg ggacatcgca tcgtgaacga
      841 gacggcgctc gcggcgctgc cggaggattt tccggcattc gtgcgcgacg cggaggcggc
      901 ggagcgggtg gcgtttctcg ccggtgagcc ggatcgttgg cgcaacgtgc cggatcttcc
      961 gatcaagcag gcgaacgggc tcgatcatta cctcgatatc gagcatctga tcgatgcggg
     1021 gatggacgtc gcgaagctgc cgtc
//
LOCUS       DASPLT010000152         1211 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_186219, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000152 DASPLT010000000
VERSION     DASPLT010000152.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1211)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1211)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1211
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_186219"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1211)
                     /locus_tag="VEA63_01610"
     CDS             complement(<1..>1211)
                     /locus_tag="VEA63_01610"
                     /inference="COORDINATES: protein motif:HMM:NF012640.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="anthranilate synthase component I family
                     protein"
                     /protein_id="HYD82706.1"
                     /translation="GGLLIERRKGSPLEILRTLLAESRAPKLPGHLAMTGGLIGVFGY
                     DLVRTWENLPLSARRDLDVPLYAFVEARELFVYDHAERTLFIIVWREVTADGGSLAAL
                     FQSAGDAAQRAYARWQQRCASTPSHKRQHRDPVLAAPADASISPQDFQSAVRRALDYI
                     AAGHTYQVNLSRRTSRATALAPETIYESLRRVNPSPYMGLLRLPEFALVCGSPELLVK
                     LVDGVVSSRPIAGTRPRGEAPPEDSHFSAELFASEKERAEHLMLVDLIRNDIGRVAAF
                     GSVHVPEFMALERYSHVMHIVSQVEGRLAPGKTWVDVLQSMFPGGTITGCPKFRTMEI
                     IEELEPVGRGFYTGALGWISFNGEMEMNIVIRSMLLQHGIAHVQAGAGIVADSRPDRE
                     LDEATRKAQAL"
BASE COUNT          242 a          375 c          393 g          201 t
ORIGIN      
        1 agagagcctg cgcttttcgc gtggcttcgt cgagttcgcg atccggtcgc gaatccgcca
       61 cgatcccggc gcccgcctgg acgtgcgcga tgccgtgctg caggagcatc gaacgaatca
      121 cgatgttcat ttccatttcg ccgttgaagc tgatccagcc gagggcgccg gtgtagaagc
      181 cgcgaccgac cggctcgagt tcctcgatga tctccatcgt gcgaaacttc ggacaacctg
      241 tgatcgtgcc gccgggaaac atcgattgca gaacgtcgac ccaggttttg ccgggcgcga
      301 gccggccttc gacttgtgaa acaatgtgca tcacgtgcga gtagcgctcg agcgccatga
      361 actcgggcac gtgcacgctg ccgaacgccg cgacccgtcc gatatcgttg cgaatcagat
      421 cgaccagcat gagatgctcg gcgcgttcct tttcgctggc gaacagctcg gcggaaaaat
      481 gcgaatcctc cggcggcgcc tcgccgcgcg ggcgcgtgcc cgcaatcgga cgcgaactca
      541 cgacgccatc gacgagcttc accagcaatt cgggcgaacc gcacacgagg gcaaactccg
      601 gcaatcgcaa caagcccatg taaggcgacg ggttcacgcg acgcagcgac tcgtagatcg
      661 tctcgggagc gagcgcggtc gcacgactcg tgcggcgcga gaggttgacc tgataagtgt
      721 ggccggcggc gatgtaatcg agcgcgcgac gaacggcgga ttggaagtct tgcggtgaaa
      781 tcgaagcgtc cgctggcgcg gcgagaacag gatcgcgatg ttgacgcttg tgcgacggcg
      841 tcgaggcgca gcgttgttgc caccgagcgt aagcgcgctg cgcggcgtcg ccagcggatt
      901 gaaacaacgc ggcgagcgaa ccgccgtcag ccgtgacttc gcgccacact ataataaaga
      961 gcgttcgttc ggcgtggtcg tagacgaaca attcgcgcgc ttcgacgaac gcatagagcg
     1021 ggacgtcgag atcgcgccgg gccgaaagcg gcaagttttc ccacgtgcga acaagatcat
     1081 agccgaagac gccgatcagc ccgccggtca tcgcgaggtg gccaggcagc ttcggcgcgc
     1141 ggctttccgc gagcagcgtt cgcaggatct cgagcggaga acccttccgg cgttcgatca
     1201 acaatccgcc g
//
LOCUS       DASPLT010000153         2305 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_63280, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000153 DASPLT010000000
VERSION     DASPLT010000153.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 2305)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2305)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2305
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_63280"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..238
                     /locus_tag="VEA63_01615"
     CDS             <1..238
                     /locus_tag="VEA63_01615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012077405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HYD82707.1"
                     /translation="FQRAVAKAAVDAGIDKRVTPHVLRHSFATHLLESGADIRTVQEL
                     LGHESVETTQIYTHVMQKPGLGVRSPLDAMSGVR"
     gene            complement(1021..1848)
                     /locus_tag="VEA63_01620"
     CDS             complement(1021..1848)
                     /locus_tag="VEA63_01620"
                     /inference="COORDINATES: protein motif:HMM:NF033580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HYD82708.1"
                     /translation="MHAEANIVDLVHGAETSEQCDVGATRSGARGGERSTGCAAGLGR
                     SRVCRSGPVSGTDGSAVAGFAGGIWSLERRIHAIPPLGESRSLAGVMGGVIPRGDACA
                     TRRLRRQHQHSCASPRGWGTQKNGADQALGRSRGGLTTKLHAASTDEHTAVALTLTAG
                     ERHDLIGFDALYAQAKEGGTLRRLTADRAYDADDLRTRLRKDDIECVIPPRKNRRRPP
                     HCSRRLYRRRHKIENWFRKIQDFRRLATRYEKLAICYLALVHLVSSVILLRSFVNTP"
BASE COUNT          425 a          690 c          727 g          463 t
ORIGIN      
        1 ttttcaacgc gccgtggcaa aagcagcggt ggatgccggc atcgacaaac gcgtcacgcc
       61 gcatgtgctg cggcactcct ttgcgacgca cttgctcgaa agcggcgcag acatccgcac
      121 ggtgcaggaa ttgctcggtc acgagagcgt ggagaccacg cagatctaca cgcacgtcat
      181 gcaaaagccc ggactcggcg tgcgcagtcc gctagacgcc atgagcggcg tgcgttgagg
      241 gacgcgcacc ggcggcgagc ggtgggcgag agactgtagg cagtggacaa tcacggcagg
      301 ccagagcgga cgacggggct ccatggctcg actgaagggg aggaagaagc ttagccgaat
      361 tcatgcgatc gaaggtggag tccgacgacg ttgtcgggct gtttgtgagt gaaacgtctt
      421 caagcccgac agaggacgtc gggctccacc tttcggggtg cagttttctc gacggccaga
      481 cggtttccac tgcgtgagtc ccgctcagct cgtgatcggg gtgcgaggtg gtagccggag
      541 gccttgaccg ggcaaagttt gattacggac gtcaaagttt tgaaccgctc atctgcgctg
      601 atttttcaaa ggacggtgac gcaagcgaac gcgtgtgtgc ttcgcctgat cggcgaagcg
      661 ccgaggtttt ggctctgccg ggatcccatt ggtagggtgt cccactggta tctttagtaa
      721 ttgagcattc ttctccacgg agcggatgtc tcggagaggc atccctaccg gcgcgtccgc
      781 gctattggtg cgcttgcgcg ccacgttgcg cgcgagcgcg caacctgctg cgggcaggca
      841 ggcgcggagg cctgccgcta cgttcgccgg ctacgacttt atttgggcaa ctctaacgat
      901 ggtgatcgat cgtgcgccgg cgaagcgggg agggagaatc gcctgcaagc gggctcctgc
      961 cttcgaagtg aggacgatag cgaagaccga aggagatcgt ccgatcggcc gggtgttctt
     1021 ctagggagtg ttaacgaaag aacgaagtag aattacgcta ctgacgagat gaacaagggc
     1081 gagatagcag atggcgagtt tctcgtaacg cgtggccaag cggcggaagt cttggatttt
     1141 ccgaaaccag ttctcgatct tgtgtcgtcg gcgatagagc cggcgcgagc agtgcggtgg
     1201 ccggcgccgg tttttgcgcg gaggaattac gcactcgatg tcgtccttgc gcaaccgcgt
     1261 gcgcaagtcg tcggcatcgt aggcgcggtc ggccgtgagg cgcctcagcg tgccaccctc
     1321 cttcgcttgg gcgtaaagag cgtcgaaacc gatcagatcg tgacgctctc cggctgtcag
     1381 cgtcagcgcc acggcggtat gctcatcggt gcttgcggcg tgcagtttcg tggtcaaacc
     1441 gccgcgcgag cgccccagag cctgatcagc gccgtttttt tgggtgcccc agccgcgtgg
     1501 ggatgcacag gaatgctggt gctgtcgacg aagacgtcga gtagcgcagg cgtcgccccg
     1561 cgggataaca cctcccataa cccctgccaa actccggctt tctcccagcg gcggaatcgc
     1621 atgtatacgg cgctccaggc tccaaattcc tccggcaaat cccgccacgg cagacccgtc
     1681 cgtgccagat acaggaccgc ttcgacaaac tctcgatctt ccaagcccgg cggcgcaccc
     1741 cgtggatctt tcacctccgc gagcgccgct gcgagttgct cccacatcgc attgttcaga
     1801 agtctccgcg ccatgcacca aatctacaat attcgcttca gcgtgcatag cctattcgtt
     1861 aacactccct agattcccca aggtttcatg agcggaaatc agcgtcgatg agcggttagt
     1921 cctgttgcca tgcgtgagcg ttgatgagcg gttcaccgtt ttgtggttcg ggcgaaaatc
     1981 tccccggggg gcgcagattt gacgtgcggc ggaggcgctc gcattaatag cgtccacgaa
     2041 ccgtgtttca cttgggaagc acggccgctc ttttcgcaac cgcgacgcgc cggggccgtc
     2101 ctgctgcttc tcacctgttc gccccttcat tcgttctccc catgtcatcg tatacctccc
     2161 tgaatcgccg cgatttcctg aaattttccg ccgcagtcgc cgcgctgctg agctttcccg
     2221 gctgcaccag cctgcaacag agcccgcgcc ggccgcgcgc gatcgctccc ggcgcgaaga
     2281 tccgcatcgc gcagatcggg tgcgg
//
LOCUS       DASPLT010000154         1850 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_166022, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000154 DASPLT010000000
VERSION     DASPLT010000154.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1850)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1850)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1850
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_166022"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..375
                     /locus_tag="VEA63_01625"
     CDS             <1..375
                     /locus_tag="VEA63_01625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009509479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HYD82709.1"
                     /translation="PAVFKREDSVLLRRKHLDRAHAFFEKYGGRAIILARFVPIVRTF
                     VPFIAGVGHMTYPRFLAYNVIGGFIWIYFFVYAGFAFGNQPFVQKNFKLVILVIIALS
                     LLPIVYEAWRGWIEHRKKPAAS"
     gene            452..526
                     /locus_tag="VEA63_01630"
     tRNA            452..526
                     /locus_tag="VEA63_01630"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:485..487,aa:Arg,seq:ccg)
     gene            complement(637..>1850)
                     /gene="lpdA"
                     /locus_tag="VEA63_01635"
     CDS             complement(637..>1850)
                     /gene="lpdA"
                     /locus_tag="VEA63_01635"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007363008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HYD82710.1"
                     /translation="KIKKAETFGITAKDVTFDFAKVMERSRGVAGQMAKGVEFLFKKN
                     KVDYIVGRAQVPAAGMVEITEGESKGKFLKAKNILIATGCKMRRIPDVAVDGVRVMTS
                     REALANTTLPKSILIVGAGAIGVEFAYFYNAFGTKVTLVEMMPQIVPVEDEDVAKTLH
                     RAFEKQGIVIKTGTKCENFRVGKNSVKVDLTKEGAAKEEIEVETVLSAIGVVANIEGV
                     IGQSVKVELDRNYVKVGDDYQTNVKGIYAAGDIIGPPWLAHVATFEAVSAVNGMFGFE
                     KPHRVKNFPGCTYCQPQISSTGLTEKAAKEKKLDYKVGKFPFTASGKAVASAESEGFV
                     KVISDAKTGEIYGAHIIGSEATELIAEYGLAVQLEATIEEIHQTIHAHPTLSEAVMEA
                     AAATTGQAIHI"
BASE COUNT          312 a          665 c          447 g          426 t
ORIGIN      
        1 cccgccgtct tcaagcgcga agattccgtt ttgctccgac gaaaacatct cgatcgcgcg
       61 cacgcctttt tcgaaaaata cggcggccgc gccatcatcc tcgcccgctt cgtcccaatc
      121 gtgcgcacct tcgtgccgtt catcgccggc gtcggccaca tgacctatcc gcgctttctc
      181 gcctacaacg tcatcggtgg tttcatctgg atctactttt tcgtctacgc cggtttcgca
      241 ttcggcaacc agccgttcgt ccaaaagaac ttcaagctcg tgatcctcgt gatcatcgcg
      301 ctctcgcttc tgccgatcgt ctacgaagcc tggcgcggct ggatcgaaca ccggaaaaag
      361 cccgcggctt cctgacccgc cgcgagccgg tcgttctttc cgctcgacag cgcgacgcct
      421 gcgcatgaat ttcgcgccgt tcttcacctc tgcgggcgta gcacaatgga tagtgtagcg
      481 gtctccgaag ccgtcgatcc gggttcgact cccggcgctc gcaccaaatt tcaagtaggg
      541 cgggtttcaa cccgcccctg aacggtggag cccgacggga tgtcggtctc cagcaacttc
      601 aagacagctg cctcgcttcg cgctcggccg ccgtcctcag atgtgaatcg cctgccccgt
      661 cgtcgccgcg gcggcttcca tcaccgcttc gctcaacgtc ggatgcgcat ggatcgtctg
      721 gtgaatctcc tcgatcgtcg cctcgagttg caccgccagc ccgtattccg cgatcaactc
      781 ggtcgcctcc gaaccgatga tgtgcgcgcc gtaaatctca ccggtcttcg catcgctgat
      841 gactttcacg aagccctcgc tctccgctga cgcgaccgcc ttgcccgacg ccgtgaacgg
      901 aaacttgccg accttgtagt cgagcttctt ttccttcgcc gccttctccg tcaaacccgt
      961 gctcgaaatc tgcggctggc aatacgtgca gcccggaaaa tttttcaccc gatgcggttt
     1021 ttcgaaaccg aacatcccgt tcaccgcgct caccgcttcg aacgtcgcca cgtgcgccag
     1081 ccacggcggc ccgatgatgt cgcccgctgc ataaatccct ttcacgttcg tctgataatc
     1141 gtcacccact ttcacgtagt tgcgatcgag ttcaaccttc acgctctggc cgatgacgcc
     1201 ctcgatgttc gcgacgacgc cgatcgccga aagcacggtc tcgacttcga tttcctcctt
     1261 cgccgcgcct tccttcgtga gatcgacctt caccgagttt ttcccgacac ggaaattctc
     1321 acacttcgtg cccgtcttga tgacgatgcc ctgcttctcg aacgcccggt gcagcgtctt
     1381 cgcgacgtcc tcgtcctcca ccggcacgat ctgcggcatc atctcgacga gcgtcacctt
     1441 cgtcccaaac gcattgtaga aatacgcgaa ctccacgccg atcgcgcccg cgccgacgat
     1501 caggatcgat ttcggcagcg tcgtgttcgc cagcgcttcg cgtgacgtca tcacacgcac
     1561 gccgtcgacc gccacgtccg ggatgcgccg catcttgcag cccgtcgcga tcaagatgtt
     1621 tttcgccttg agaaacttcc ctttgctctc gccttccgtg atctcgacca tccccgccgc
     1681 cggcacttgc gcgcgaccga cgatgtaatc gaccttgttc ttcttgaaca aaaactcgac
     1741 gcctttcgcc atttgcccgg cgacaccgcg cgagcgctcc attaccttcg cgaaatcgaa
     1801 cgtcacgtcc ttcgccgtga tgccaaacgt ctccgctttt ttgatcttct
//
LOCUS       DASPLT010000155         1865 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_247813, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000155 DASPLT010000000
VERSION     DASPLT010000155.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1865)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1865)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1865
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_247813"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..319)
                     /locus_tag="VEA63_01640"
     CDS             complement(<1..319)
                     /locus_tag="VEA63_01640"
                     /inference="COORDINATES: protein motif:HMM:NF020252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HYD82711.1"
                     /translation="MTLDRAAIVTLGSKLAPAAATFGRLRGMLEDPNADTKEVVDLIR
                     LDPALTFHVIRMSNSVIFGLREQNNSLEDAVGRVGFRELYRLVGLAAAQQVCQRDLVH
                     YRLR"
     gene            complement(530..1246)
                     /locus_tag="VEA63_01645"
     CDS             complement(530..1246)
                     /locus_tag="VEA63_01645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD82712.1"
                     /translation="MIRWWMLLFFFAPGVLMRADQTALLRQVVDAWLADRDRWAFTQT
                     VKEYDGRALKEERVERYDPSRGYAARWELISINGRKPTPDELAEWTKRKNKKQRKRRA
                     DAAENFDFENAKVIEENARRIRYELPLRSGVEWLFPISKVELLLTIDKSGPALEQVQA
                     RISEPFRVALGLARILDVDLDVQMQPPPAADPADAKPSGAGQAVVTKFGDRVEYAWSD
                     FERVADSAIEEERARALKSE"
     gene            complement(1434..>1865)
                     /locus_tag="VEA63_01650"
     CDS             complement(1434..>1865)
                     /locus_tag="VEA63_01650"
                     /EC_number="4.1.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004611 - phosphoenolpyruvate
                     carboxykinase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase
                     domain-containing protein"
                     /protein_id="HYD82713.1"
                     /translation="RDPMAMLPFCGYHMGDYFRHWIKMQRTLSETPRVFHVNWFRKDK
                     DGNFMWPGFGENMRVLKWIVDRARGRARGRETPIGWQPRYEELDWTGLDYAKETFDEL
                     QAFDRAAWRVEVIGHEELFIDLHDHLPKELVYERELLICRM"
BASE COUNT          343 a          701 c          483 g          338 t
ORIGIN      
        1 cgcgcagccg gtagtgcacc agatcgcgct ggcaaacctg ctgtgccgcc gcgagtccga
       61 ccaaccggta aagctcgcga aaacccaccc ggcccaccgc gtcttcgagc gaattgttct
      121 gctcgcgcaa accgaagatc acgctgttgc tcatccggat cacgtgaaat gtcagcgccg
      181 gatcgaggcg gatcaggtcc acgacttcct tggtgtcggc attcggatcc tcgagcatgc
      241 cgcgcagccg cccaaacgtg gccgcagccg gagcgagttt actaccgaga gtgacgatgg
      301 cggcgcgatc gagtgtcata gacgggaacc cgcctctcct cgtcacaaag ccgcgcctcc
      361 gtaagtaaat ttttcgataa cccgcgaccc tcgcggcccc gtccagcagg tggaacccaa
      421 cgtcacgccg ggttgctcgc gcccccaccc gtcattcaca acgaacgcgc tcccttactc
      481 gccgcgcaac acgcccgctc atccggcgtt cttcaccacg cgcccgccgt cactcgctct
      541 tgagcgcccg cgcgcgctcc tcctcgatcg ccgaatcggc cacgcgttcg aaatcgctcc
      601 acgcatactc cacgcgatcg ccgaacttcg tcaccaccgc ctgacccgcc ccgctcggct
      661 tggcgtctgc cggatcggcc gccggcggcg gttgcatctg cacatcgaga tcgacgtcga
      721 gaatgcgcgc cagcccgagc gcgacccgaa acggttcgct gatccgcgcc tgcacctgct
      781 cgagcgccgg tcccgatttg tcgatggtga gcagcagctc gaccttgctg atgggaaaca
      841 accactcgac accgctgcgc aacggcagct cgtagcgaat cctccgcgca ttctcctcga
      901 tcaccttcgc gttctcgaaa tcgaaattct cggccgcatc agcgcgtctt ttccgctgct
      961 ttttgttttt gcgcttcgtc cactcggcga gctcgtcggg cgtcggtttg cgaccgttga
     1021 tcgagatcag ctcccaacgc gccgcgtaac cccgcgacgg atcgtagcgt tccactcgct
     1081 cctctttgag cgcgcgtcca tcatactcct tcaccgtctg cgtaaacgcc cagcggtcgc
     1141 gatccgccag ccacgcatcg acaacctgcc gcagcaacgc cgtctgatcc gcccgcatga
     1201 gcaccccagg cgcgaagaaa aaaagcagca tccaccagcg tatcatccgc tcaccgactc
     1261 cccgacgcac aaccggttcc gcgcaggccg ggtgcacagg ggcagagcct gcttgcaggc
     1321 gatttccggt ttccgtgtgg ctcgctgcgt gagcagcggg atcgccatcg cctgcaagca
     1381 ggctcctacc tcagactcaa ccagtccgcc gacgagcgcg tttcctcgcg cccttacatc
     1441 cggcaaatca acaactcgcg ctcatacacc agttccttcg gcaggtgatc gtgcaaatcg
     1501 atgaacagct cctcgtgccc gattacctcc acgcgccacg ccgcccgatc gaacgcctgc
     1561 aactcgtcga acgtctcctt cgcgtaatcg agccccgtcc aatcgagctc ctcgtagcgc
     1621 ggctgccatc cgatcggcgt ctcgcggccg cgcgcccgcc cgcgcgcgcg atccacgatc
     1681 cacttcaaca cgcgcatatt ttcgccaaag cccggccaca tgaaattccc gtctttgtcc
     1741 ttccgaaacc agttcacgtg aaacacccgc ggcgtctcgc tcagcgtccg ctgcatcttg
     1801 atccagtggc gaaaataatc gcccatgtga taaccgcaga acggcagcat cgccatcgga
     1861 tcgcg
//
LOCUS       DASPLT010000156         1684 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_186293, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000156 DASPLT010000000
VERSION     DASPLT010000156.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1684)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1684)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1684
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_186293"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..552)
                     /locus_tag="VEA63_01655"
     CDS             complement(<1..552)
                     /locus_tag="VEA63_01655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007359732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HYD82714.1"
                     /translation="MHHFRYSGQDLHCESVDLAAVAKLYGTPTYVYSASTISDNFTRL
                     QKSLSGLDLQICYAMKANSNLALLRLFSNLGAGYDLVSGGEIRRVIAAGGNVRSSVFA
                     GVGKTEAEIKLALENEVFAFHVESEPELARINHVAGTLGVKAPIAIRVNPDVDAHTHA
                     KITTGKSDNKFGIPLKAAAAAYEA"
     gene            complement(627..872)
                     /locus_tag="VEA63_01660"
     CDS             complement(627..872)
                     /locus_tag="VEA63_01660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD82715.1"
                     /translation="MGAFDGDPGGAAGEQEGQTVANEFAAAQDGAVKIDLVATAIAAG
                     EEAGELRVPVTTARVTTQAKTDGRTVTEAVQKAEHTL"
     gene            1002..1487
                     /locus_tag="VEA63_01665"
     CDS             1002..1487
                     /locus_tag="VEA63_01665"
                     /inference="COORDINATES: protein motif:HMM:NF015147.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LEA type 2 family protein"
                     /protein_id="HYD82716.1"
                     /translation="MNSLSCRLLFLLVASFFFAGCSGVKLGGVTATLIDFKPTQATLL
                     ESTGTLTIRYTNENISPLGFSGSSHKLYLNGSYVGKAVSDQPFGIPPLNTVTQDVTIH
                     FENLSLVRQLLAVRETQTAAYRLESVIFQTVYEEEYEYKTRSEGSLDLRGVSEAIGGK
                     Q"
BASE COUNT          317 a          589 c          466 g          312 t
ORIGIN      
        1 cgcctcgtaa gccgccgccg ccgccttcag cgggatgccg aacttgttgt cgctctttcc
       61 cgtcgtgatc ttcgcatgcg tgtgcgcatc gacgtccggg ttcacgcgaa tcgcgatcgg
      121 cgccttcacg cccagcgtgc cggccacatg attgatccgc gcgagctccg gttcgctctc
      181 gacgtgaaac gcgaaaacct cgttctccag cgccagcttg atctccgcct cggtcttgcc
      241 gacgcctgcg aacacgctcg aacgcacatt gccgcccgcc gcgatcacac gccggatctc
      301 gccgccgctc accaggtcgt agcccgcgcc gagattcgaa aacagacgca gcagcgccag
      361 attcgagttc gccttcatcg cataacagat ctgcagatcg agacccgaaa ggctcttctg
      421 cagccgggtg aaattatcgg aaatcgtcga cgcgctgtag acgtaggtcg gcgtcccgta
      481 gagtttggcg acagcggcga gatcgacgga ctcgcagtgc agatcctgac cggaatagcg
      541 gaagtggtgc atggaagcgg gaaaattttc gattaaacgc gcacgttagt gagaattccg
      601 ttgaagaaac gactccaaac ttccgattaa agggtgtgct ccgctttctg caccgcctct
      661 gtcaccgtcc ggccatccgt cttggcttgg gtcgtgacgc gcgcggtcgt taccggaaca
      721 cgcaattcgc cagcttcctc gccagcagca atcgcggtcg caacgagatc gatcttcacg
      781 gcgccatcct gcgcggccgc aaattcgttc gccacggtct gaccctcctg ctcgccggcg
      841 gcgccgcctg gatcgccgtc gaaagcgccc aagcgctctc gatattctga ggtcgagccc
      901 gacgtcgctc gccttttcgt gtagggctgc tgcttgcggc acgcttcgtc cgctccgcag
      961 ccttgcagtt tccgctcacg ctgccatcgg tggtcacacc catgaactcc ctcagctgcc
     1021 gcctgttgtt cctcctcgtt gcctcgttct ttttcgccgg ctgctccggg gtaaagctcg
     1081 gcggagtcac cgcgacgctc atcgatttca aacccacgca agccaccctg ctcgagagca
     1141 ccggcacgct gacgattcgc tacaccaacg agaacatctc gccgctcggc ttctccggct
     1201 cgtctcacaa gctgtatttg aacggcagct acgtcggcaa ggccgtgagc gaccagccgt
     1261 ttgggatccc gccgctgaat acggtcacgc aggacgtcac catccacttc gaaaatctct
     1321 cgctcgtgcg ccagctcctc gccgtccgcg aaacgcagac cgccgcctac cgtctcgaga
     1381 gcgtgatctt ccagaccgtc tacgaagagg aatacgaata caaaacccgc tccgaaggct
     1441 ccctcgatct ccgcggcgtc agcgaagcca tcggcggcaa acagtaacaa agcggccccc
     1501 gccgcgcacc ggcggacggc ctaaaacggc ccgcgaagcc gcaacaggga ccgcctccag
     1561 tgctggctgg cagctaatca aaccgccagc cgaagatgcg agcggtgcgc cgaggccccg
     1621 ccggttccgg cgggcgcaaa ctcgaaaacc gccaacggcg tcttcggacg tgcataatcc
     1681 gccg
//
LOCUS       DASPLT010000157         1270 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_268198, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000157 DASPLT010000000
VERSION     DASPLT010000157.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1270)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1270)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1270
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_268198"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..706)
                     /locus_tag="VEA63_01670"
     CDS             complement(<1..706)
                     /locus_tag="VEA63_01670"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HYD82717.1"
                     /translation="MILLGVNIDHCATLRQARYRDAGATVGGAIEPDPVTFALLAERA
                     GADGITVHLREDRRHIQERDVWRLRESIATRLNFEMACTPAMTEFALKLKPESVCLVP
                     ENRQEVTTEGGLDVVGNRERVRACVDAMNAAGIRTSLFIDPDEAQIELAAKLSAPWVE
                     LHTGAYANEYGDATGRAREFQRLRLGAVRAHELGLVVNAGHGINYVNIAEVRTLPHLH
                     ELNIGHSIVSRALFTGV"
     gene            799..>1270
                     /locus_tag="VEA63_01675"
     CDS             799..>1270
                     /locus_tag="VEA63_01675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VC0807 family protein"
                     /protein_id="HYD82718.1"
                     /translation="MSKPPQKRENLLVNLLFNIAIPALVLSKLSTPERLGPVVGLVVA
                     LAFPLGYGVMDFLQRRQANFVSVIGFVSILLTDGLGLMQIGGFWFAVKEAAVPAVIGA
                     AVLLSLKAKRPLVHTFLYNDQVIDVAKIDSALAARGTRSGFDRLLVTSSYLLALS"
BASE COUNT          209 a          433 c          385 g          243 t
ORIGIN      
        1 ccacgccggt gaacaacgcc cggcttacga tgctgtgccc gatgttgagc tcatgcagat
       61 gcggcagcgt gcgcacttcg gcgatgttca cgtagttgat gccgtgaccg gcgttcacca
      121 cgaggccgag ctcgtgcgcg cgaacggcgc cgaggcgaag ccgttgaaac tcgcgcgcgc
      181 ggcccgtcgc gtcgccatac tcgttcgcat acgcgccggt gtgcaactcc acccacggcg
      241 cgctgagttt cgccgcgagt tcgatctgcg cttcgtcggg atcgatgaaa aggctcgtcc
      301 tgatgcccgc cgcgttcatc gcgtcaacgc acgcgcgcac acgctcacga ttacccacca
      361 catcgagccc gccctcggtc gtcacctcct ggcggttctc cggcacgagg caaaccgact
      421 cgggcttcaa tttcagcgcg aactccgtca tcgcgggcgt gcaggccatt tcgaaattca
      481 accgcgtggc gatcgactcg cgcagccgcc acacgtcgcg ctcctgaatg tggcgacgat
      541 cttcgcgcag gtgcaccgtg atgccatcgg cgcccgcccg ctccgcgagc aacgcgaagg
      601 tcaccggatc cggttcgatc gcgccaccga cggtggcgcc cgcgtcacga tagcgcgcct
      661 gtcggagcgt agcacagtgg tcgatgttga cgccgaggag gatcatcgaa agtccggcac
      721 tatagaatcg ctctcgcaga aaccaagaaa tgaatcgccg cgtgccgcgc ttcgtggctt
      781 gctgcccgac tttccgccgt gagcaaacct cctcagaaac gcgaaaacct gctggtcaac
      841 cttctcttca acatcgcgat tcccgcgctc gtgttgtcga agctcagcac gccggaacgg
      901 ctcggacctg tcgttggcct ggttgtcgcg ctcgcgtttc cgcttggcta cggcgtgatg
      961 gattttctcc agcggcgcca ggcgaacttc gtttcggtca tcggcttcgt cagcatcctg
     1021 ctcaccgacg gtctcggcct gatgcagatc ggcgggttct ggttcgcggt gaaggaagca
     1081 gccgtcccgg cggtgatcgg cgccgcggtg ctgttgtcgc tgaaagcgaa gcgcccgctc
     1141 gtgcacacgt ttctctacaa cgaccaggtc atcgacgtcg ccaagatcga cagcgcgctc
     1201 gccgcgcgcg gcacgcgcag cggtttcgac cgcctgctgg tcacgtcgag ttatctgctc
     1261 gcgctgtcgt
//
LOCUS       DASPLT010000158         1161 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_186309, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000158 DASPLT010000000
VERSION     DASPLT010000158.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1161)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1161)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1161
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_186309"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1161
                     /locus_tag="VEA63_01680"
     CDS             <1..>1161
                     /locus_tag="VEA63_01680"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006206 - pyrimidine nucleobase metabolic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HYD82719.1"
                     /translation="ADYQLSALLMAIVLRGMNREEVVAYTEAMMRSGTVADLSSVVRP
                     KADKHSTGGVGDKISLHLAPMVAACGVAVPMISGRGLGHTGGTLDKLEAIPGFRVNLS
                     MAEYRAQLERIGLALIGQTAELAPADKKIYALRDVTATVESIPLICGSILAKKLAEGI
                     DVLVLDVKFGRGAFMKDKAQARELAEALVSVAKAMGKPTCAVLTAMDQPLGRSVGNAL
                     EVIESIECLRGEGPEDTMEVTYALGEQMLLLANVAKDAKDARSQLERSVSSGAALKKL
                     GDMIAAQGGDPHVVDDLNRLPRARLAEPFVAVSEGVVADVDAMGVAFAALHLGAGRAK
                     AEDDVDHAVGINGLVKVGEAVARGAPLCVVHANNEQALAEAKERLAQAITIG"
BASE COUNT          234 a          285 c          447 g          195 t
ORIGIN      
        1 gggcggatta tcaactctcg gcgttgttga tggcgatcgt gctgcgcggg atgaatcgcg
       61 aggaggttgt cgcttacacg gaagcgatga tgcggtcggg gacggtggcg gatctgagca
      121 gcgttgtgcg accgaaggcg gacaagcatt ccacgggcgg agtcggcgat aaaatctcgc
      181 tgcatctcgc gccgatggtg gcggcgtgcg gtgtcgcggt gccgatgatt tcgggccgcg
      241 ggctcggaca cacgggcgga acgctggata agttggaagc gattccgggc tttcgcgtga
      301 acctctcgat ggcggagtat cgcgcacaac tcgagcgcat cgggctggcg ctgatcggac
      361 aaacggcgga gctggcgcca gcggacaaaa agatctacgc gctgcgcgat gtaacggcga
      421 cggtggagag tatcccgctg atttgcggaa gcattttggc gaagaagctc gcggagggaa
      481 tcgatgtgct cgtgctcgat gtgaagttcg gccgcggcgc attcatgaaa gacaaagcgc
      541 aagcgcgcga gctggcggaa gcgctggtgt cggtcgcgaa agcgatgggc aagcccacgt
      601 gtgccgtgct gaccgcgatg gatcagccgc tagggcgctc ggtggggaat gcgttggaag
      661 tgatcgaatc gatcgaatgc ctgcgcggcg aaggtccaga agacacgatg gaggtgacgt
      721 atgcgttggg tgaacagatg ctgctgctcg cgaacgttgc gaaagacgca aaggacgcgc
      781 gcagccaatt ggagcgaagt gtgagcagcg gcgcggcgct gaaaaaactc ggcgacatga
      841 ttgcggcgca gggaggcgac ccgcatgtcg tggatgattt gaaccggttg ccgcgagcga
      901 ggcttgcgga gccatttgtg gcggtgagcg aaggcgtggt ggccgacgtg gatgccatgg
      961 gtgtagcgtt cgcggcacta catctcggcg cgggccgggc gaaggcggag gacgatgtgg
     1021 atcacgcggt cggcatcaac ggactcgtca aagtcggcga agccgtggcg cgaggtgcgc
     1081 cgttgtgcgt ggtgcacgcg aacaatgagc aagcgctcgc ggaagcgaag gagagactcg
     1141 cgcaagcgat caccatcggc g
//
LOCUS       DASPLT010000159         2017 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_186311, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000159 DASPLT010000000
VERSION     DASPLT010000159.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 2017)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2017)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2017
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_186311"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(108..1211)
                     /locus_tag="VEA63_01685"
     CDS             complement(108..1211)
                     /locus_tag="VEA63_01685"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HYD82720.1"
                     /translation="MKKILIALVVLVAAAFALSSFLKTTAKVAVATPGRAINSVSGSV
                     TVRAEYQMELKSEGGGRVIASELEPGLPVTKGMVLMQIDTTDLELEIAQIESDYAAKK
                     KSLEIGSATRVTLMTARENLENYERLAESGNYPQVELERRRREVNQIEQSLALEQIAN
                     EHQLATFENTLKVKRRQLEKMTVRAPFDGVVSEVLARPGDLVGGGAPLAILISTSRTV
                     EAKVSEENFAGVRIGQQAHVRFLGYGDALYEASVKQILPTADPATQRYIVHLDVKIER
                     EKLIPGITGEVTIVVAERQSDTIIPRRALFGNHVLVVENGRVQLRSVKVGYISLTAVE
                     ILEGLATGDRVIVEELERFRDGDRVKIVEEPAR"
     gene            complement(1208..>2017)
                     /locus_tag="VEA63_01690"
     CDS             complement(1208..>2017)
                     /locus_tag="VEA63_01690"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HYD82721.1"
                     /translation="GEISEADLYSPTVNFDRTQLDLERVIEDYEWSKKTFAKLYGGEP
                     LRDEQIPEAIPDVALEFDGFNAMLGEFAQEKEQRSFGLQNLQRDIEIEKLNLDIASTR
                     LKPKVNLLVGTSQDEQSYTRNIGDKYGVTAYYVGVRLEWSIFDGFATKAIKSNSVIRR
                     RQLEHAYRETSASLLDNARVKLKQLEFTARSLAIDRKGLELNEENLERVRDNVARGLS
                     APSELRRAEYNHAGALINTYNARADFLVRTSDFLATILVDPAIAQLQANLR"
BASE COUNT          359 a          635 c          591 g          432 t
ORIGIN      
        1 agaacgatgc acgacaggct catcgccatg gcacgcttct tcgccgtgag aaaccggaac
       61 gcgatgcgta aattgggaga catgtccgca gcgaaaactc ggccggctca gcgcgccggc
      121 tcttccacga tcttcacccg gtcaccgtcg cggaagcgtt cgagctcctc gacgatcacg
      181 cggtcgcctg tcgcgagccc ttcgagaatt tcgaccgcgg tgaggctgat gtagccgacc
      241 tttacgctgc gcaactgcac ccggccgttc tcgacgacga gcacgtggtt gccgaacaac
      301 gcgcggcgcg ggataatcgt atcagactgc cgctcggcga cgacgatcgt cacctcgccc
      361 gtgatgccgg ggatgagttt ttcgcgctcg atcttcacat cgagatgaac gatgtagcgc
      421 tgcgtcgccg gatcagccgt cggcagaatt tgcttaacgg acgcttcata aagcgcgtcg
      481 ccgtagccca ggaaacgcac gtgcgcctgc tggccgatgc gcacaccggc gaagttttcc
      541 tcgctcacct tcgcttcgac ggtgcggctc gtgctgatca aaatcgccag cggcgcgccg
      601 ccaccgacca agtcgccggg gcgtgccaga acttcggata caacaccatc gaacggggcg
      661 cgcaccgtca tcttttccaa ctggcggcgt ttcaccttca gcgtgttttc gaaggtcgcg
      721 agctggtgtt cgttcgcgat ctgttccagc gcgagcgatt gctcgatctg gttcacctca
      781 cggcgccgcc gctcgagctc gacctgcgga taatttcccg actcggcgag gcgctcgtag
      841 ttctcgaggt tttcgcgagc ggtcatcaag gtcacgcgcg tggccgagcc gatttcgagg
      901 ctcttcttct tcgccgcgta gtcgctttca atttgtgcga tctcgagttc gagatcggtc
      961 gtgtcgatct gcatgagcac catccccttc gtcaccggga gaccaggctc gagttcgctg
     1021 gcgatgacgc gcccgccgcc ttcgctcttc agctccatct gatattcggc tctgaccgtg
     1081 acgctgcccg acactgagtt gatagcgcgg cccggcgtcg caacggcgac cttcgcggtc
     1141 gtttttagga agctgctcaa ggcaaacgcc gccgcgacga gcacgacgag cgcgatcagg
     1201 atttttttca tcgaagattg gcttgaagct gcgcgatcgc cggatcgacc agaatggttg
     1261 caaggaaatc cgacgtcctc accaagaagt ccgcgcgcgc gttgtaggtg ttgatcagcg
     1321 caccggcgtg attgtattcg gcgcgacgca attccgatgg cgccgagagc ccgcgcgcga
     1381 cgttatccct gacgcgctcg agattttctt cgtttagttc cagcccctta cgatcgatcg
     1441 caaggctgcg cgccgtgaac tcgagctgct tcagcttcac gcgggcgttg tcgagcaagg
     1501 acgcggacgt ctcgcgatac gcgtgttcga gctgccggcg gcgaatcacc gagttgctct
     1561 tgatcgcttt cgtcgcgaac ccgtcgaaga tcgaccattc caatcgcacg ccgacgtagt
     1621 aggcggtcac gccgtatttg tcgccgatgt tgcgcgtgta actctgctcg tcctggctcg
     1681 tgccgacgag aagattgacc ttcggcttca accgggtgct cgcgatgtcg aggttgagtt
     1741 tctcgatttc aatatcgcgc tgaagattct ggaggccgaa cgaacgctgc tccttttcct
     1801 gcgcgaactc gcccagcatc gcgttgaaac catcgaactc cagcgcaacg tcgggaatcg
     1861 cctccgggat ctgctcatcg cgcaacggct cgccaccgta gagcttcgcg aaggttttct
     1921 tcgaccattc gtagtcctcg atcacgcgtt ccagatcgag ctgcgtgcga tcgaagttca
     1981 ccgtgggcga gtaaagatcg gcctcggaga tctcgcc
//
LOCUS       DASPLT010000160         1406 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_43057, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000160 DASPLT010000000
VERSION     DASPLT010000160.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1406)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1406)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1406
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_43057"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1151
                     /locus_tag="VEA63_01695"
     CDS             <1..1151
                     /locus_tag="VEA63_01695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD82722.1"
                     /translation="IHLNQVPNPTGEGKDYGVTFNLFDGKFVAKLNRYELEEKNSRSG
                     ATSGAFAARTFRFFFDADTVLNWNPANQTFDSSDDPWDLEQMGAQWYMSANPAADPAA
                     AIQYARDTYLVAFDFDSAFIDRVRDIGLGNFAEVNTVISKGYELELSYNPTPYWTLKL
                     TGAQQQAIDSELSHNISGFFAEHLEALKAITIPTNPFTTNNGTAGVQWWASGATSATS
                     TATPQNFYFQNILTTLRQAAANEGKPRPQTREYSFAATTSYQFKGVTDHRWLKNITVG
                     GSLRWADQAALGYYGLPSTDPDVKGAIVEYDVSRPIYDDPQWSLDLMAAYEFKMYNDR
                     VRCRVQLNVQNALEDGGLRPFVYNPDGTPWNFRIIDPRRFVLSATFSL"
BASE COUNT          289 a          404 c          429 g          284 t
ORIGIN      
        1 acattcactt gaaccaggtc ccgaatccga cgggcgaggg caaggactac ggcgtcacgt
       61 tcaatctctt cgacgggaag ttcgtcgcga agttgaaccg ctacgaactc gaggagaaaa
      121 actcgcgcag cggcgcgacg agcggtgcgt tcgccgcgcg cacgttccgc ttcttcttcg
      181 atgccgacac cgtgttgaac tggaatccgg cgaaccagac cttcgacagt tccgatgatc
      241 cgtgggacct cgagcagatg ggcgcgcaat ggtatatgtc ggcgaatccg gcggcggacc
      301 cggcggctgc gatccagtat gcgcgcgaca cttacctggt ggcgtttgac ttcgactcgg
      361 cgttcatcga tcgcgtgcgc gatattggtc tcgggaattt cgccgaggtg aacacggtga
      421 tatcaaaggg ctacgagctc gaactgagct acaacccgac gccttattgg acgttgaagc
      481 tcactggcgc tcagcaacag gcgatcgact ccgaactgtc gcacaacatc agcggtttct
      541 tcgcggagca tttggaagca ttgaaggcga tcacgattcc cacgaatccg ttcacgacga
      601 acaacggcac ggctggcgtg cagtggtggg cctccggggc gacgagtgcg acgtcgacgg
      661 caacgcctca gaacttttat ttccaaaaca tcctgacgac gctgcgccag gcggcggcga
      721 acgaaggcaa gccgcgcccg cagacgcgcg agtattcgtt cgcggcgacg acgagttacc
      781 agttcaaggg cgtcaccgat catcgatggc tgaaaaatat caccgttggc ggcagcttgc
      841 gttgggcgga ccaagccgcg ctcggttact acgggctgcc ctcgacggat ccggacgtga
      901 aaggagcgat tgtcgaatac gacgtatcgc gtcccattta cgacgacccg cagtggtcgc
      961 tcgatctgat ggcggcctac gagttcaaga tgtataacga ccgcgtgcgc tgccgcgtgc
     1021 agctcaacgt gcagaacgcg ctcgaagacg gcggcctgcg gccgttcgtc tacaatccgg
     1081 atggcacgcc gtggaatttc cggatcatcg acccgcggcg gtttgtgctc tcggcgacgt
     1141 tcagcctgta agcgattttc ggtgggcgcg cgcggatttt tcgtccgcgc ggcgcgcacc
     1201 aggtcaggcg ggtcgcggac gtgccgtgcg tcttagcggg atcattcagt tgcccgcgaa
     1261 tcacgccaat tgttgcgaat acaagcgaag agaaatgatc tttcaccaaa cgatgatctg
     1321 gccttgatgc ggctacattt gattccaact cttttaaatt cgcgttcatt tgcgtcattc
     1381 gcgggaggtc ttctgcatga agacag
//
LOCUS       DASPLT010000161         1706 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_23840, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000161 DASPLT010000000
VERSION     DASPLT010000161.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1706)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1706)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1706
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_23840"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..430
                     /locus_tag="VEA63_01700"
     CDS             <1..430
                     /locus_tag="VEA63_01700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HYD82723.1"
                     /translation="WPNPASLRDLVYSRLLPVRQTVGQFLARYESALLADGLRTVTVT
                     HANALAAEGCVTSGWLRRRLAACGANIAAATFKVEPAKSGSLGIEFGYADVKKYFRWR
                     GDFTARHAEFEADFGTGRATLPAAASLLTPEMALSEAMFF"
     gene            793..1365
                     /locus_tag="VEA63_01705"
     CDS             793..1365
                     /locus_tag="VEA63_01705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HYD82724.1"
                     /translation="MFIAVIEIFKGAGVLIYPLGLCSAAAVFIICERSFALRKAAVMP
                     QDLVEAVVNGQPLMGGRHTVLARIVAFAEQHKNDEDAVKAFARLEINRMERGVPYLDV
                     IYAAAPLIGLTGTVIGLLKVFSQISPDTGLPDPVAFTSGVALALSATVIGLCIAVPSL
                     VGSGYLQRKIENYAAQLDVLLERILKRTQQ"
     gene            1362..>1706
                     /locus_tag="VEA63_01710"
     CDS             1362..>1706
                     /locus_tag="VEA63_01710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HYD82725.1"
                     /translation="MSGIYQKRRKRPELNLVPLIDVLVMLIFFAFVTMQFKSAATLNI
                     TLPKVETAGKNEFRGNVTIGIDKDGSLSFNGKPVNDTQLVELLQQVKNVDKDIPVLIR
                     SDEKATLDRLTFV"
BASE COUNT          304 a          474 c          600 g          328 t
ORIGIN      
        1 gtggccgaac ccggcctcgt tgcgcgatct ggtttattcg cggctgctgc cggtccgaca
       61 aacggtgggg caatttttgg cgcgctacga atcggcgctg ctggcggatg gcttgcggac
      121 ggtgacggtg acgcacgcga acgcgctggc cgccgagggt tgcgtgacga gtggctggct
      181 gcggcggcgc ctggcggcgt gcggcgcgaa catagcggcg gcgacgttca aggtcgagcc
      241 ggcgaagtcg ggcagcctcg ggatcgaatt cggctacgcg gacgtgaaga aatattttcg
      301 ctggcgtggg gatttcaccg cgaggcacgc ggagttcgaa gcggatttcg gcaccggccg
      361 cgcgacgctg ccggcggcgg cgagcttgct gacgccggag atggcgctga gcgaagcgat
      421 gtttttctaa gcggcatact gcgatcgaag gtggaaaccg acgcttcgtc gggctccacc
      481 gtgccgacct agcccggata gatcacacgc gtgaccgcgg cgaggcaggt agggatgcct
      541 ctccgaggca tccgcggacg gctcggagag ccgcccctac cttcgagaag acgggacgcc
      601 gggcatattc cagcgagggt gaaatatccg acctggcggc gggagaatga gaatggacgg
      661 ggagagtcgg ggcgtaaagc ccctcccacc gttgcgggcg cttgcaattc gttcatgcaa
      721 aggcatgcag ttggcgcgtt tttgtgcgaa cttttgctcg gacctcacgt cacatcgtgc
      781 accacttctc ccatgttcat tgccgtgatt gaaatcttca agggcgccgg cgttctcatt
      841 tatccgctcg ggctctgctc ggcagcggcg gtgttcatca tttgcgagcg atcgttcgcg
      901 ctgcggaaag ccgcggtgat gccgcaagat ctcgtcgagg cggtggtcaa cgggcagcct
      961 ttgatgggag ggcggcacac ggtgctggcg cggatcgtgg cctttgcgga gcagcacaag
     1021 aacgacgagg atgcggtgaa ggcgtttgcg cggctcgaga tcaatcgcat ggaacgcggc
     1081 gtgccttatc tggacgttat ttacgcggcg gcgccgctca tcggtttgac gggcacggtg
     1141 atcggtttgt tgaaggtgtt ttcgcagatc tcgcccgaca cgggcctgcc ggatccggtc
     1201 gcgttcacgt cgggcgtcgc gctggcgctg tcggcgaccg tgatcggctt gtgcatcgcg
     1261 gtgccgtcgc tggtcggcag cgggtatttg cagcgcaaga tcgagaacta cgcggcgcag
     1321 ctcgacgtgt tgctcgagcg gattttgaag cgcacgcaac aatgagcggc atttaccaga
     1381 agcggcggaa gcggcccgag ttgaatctcg tgccattgat cgacgtgctc gtgatgctga
     1441 tctttttcgc gttcgtcacg atgcagttca agtccgccgc cacgctgaac atcacgctgc
     1501 ccaaagtgga gacggccggg aaaaacgagt ttcggggcaa cgtgaccatc ggaatcgaca
     1561 aggacgggtc gctgagcttc aacggcaagc ccgtcaacga cacgcagctg gtggagctgc
     1621 tgcagcaggt gaagaacgtc gacaaggaca tcccggtgct aatccggtcc gatgagaagg
     1681 cgacgctgga tcgcctgacg ttcgtg
//
LOCUS       DASPLT010000162         1508 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_124847, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000162 DASPLT010000000
VERSION     DASPLT010000162.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1508)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1508)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1508
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_124847"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..374)
                     /locus_tag="VEA63_01715"
     CDS             complement(<1..374)
                     /locus_tag="VEA63_01715"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007363713.1"
                     /note="Catalyzes the transfer of electrons from NADH to
                     quinone; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HYD82726.1"
                     /translation="MIATLDGERVTKLKPVFGYLHRNHEKLGENTSYLGSMPYTDRLD
                     YLCSMTNNWAYALAVEKLAGQPVPDRAQYLRIILAELTRLVNHCCLVGFLFNDLGTSF
                     TPLLYAFREREKLLDLFEALSGS"
     gene            complement(536..1237)
                     /locus_tag="VEA63_01720"
     CDS             complement(536..1237)
                     /locus_tag="VEA63_01720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007363712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HYD82727.1"
                     /translation="METLDQIRDRLLARFPAAQIALSTNPGPAAEHSLLLDHAHARDI
                     SLFLRDAAGLRFDYCSNVTGIDWPEKEIVETTKTTEPDPAGGPPTIVEQKTKRLQPGY
                     LEVVYHLYSMELKHGPLVLRLRTANRTHQTIVPSFTPVWRSCEFQEREAYDLFGIVFE
                     GHPDLRRILMWDEFKDHPMRKDYVEPDDFEWEPTPHADVLARAQQHQAVGGALRPDSA
                     PTSGPKAPPTTESPT"
BASE COUNT          273 a          373 c          565 g          297 t
ORIGIN      
        1 gagccggaga gcgcttcgaa gagatcgagg agtttctcgc gctcgcgaaa cgcgtagagc
       61 agcggcgtga acgacgtgcc gaggtcgttg aagagaaagc cgacgaggca gcagtggttc
      121 accaggcgcg tgagctcggc gaggatgatc cggaggtatt gcgcgcggtc gggaacgggc
      181 tggccggcga gcttttcgac ggcgagcgcg taggcccagt tgttcgtcat cgagcagagg
      241 taatcgagac gatcggtgta gggcatcgag ccgagatacg acgtgttctc gccgagcttc
      301 tcgtgattgc ggtgaaggta gccgaagacg ggtttgagtt tcgtgacgcg ttcgccgtcg
      361 agggtggcta tcatgcgaaa cacgccgtgc gtggacgggt gttgcgggcc gagtgagact
      421 tcgaggaggt cgccgtgaaa ctcgtcgtgg atctctcgga cggtcggccc ggtgggaccg
      481 ccccacgggg cagaagctga gagctgagag cctagagctg agagcaatgg cgtgctcatg
      541 ttgggctctc ggttgtggga ggggctttag gccccgacgt tggggccgag tcggggcgta
      601 aagcccctcc cacagcttga tgctgttggg cgcgggcgag gacgtcggcg tgcggcgtcg
      661 gctcccactc gaagtcgtcg ggctccacgt agtctttgcg catcgggtgg tccttgaact
      721 cgtcccacat gaggatgcga cgcaggtcgg gatgaccttc gaaaacgatg ccgaaaagat
      781 cgtaggcctc gcgctcctgg aattcgcacg accgccacac tggggtgaaa gacgggacga
      841 tggtttggtg ggtgcgattg gcggtgcgca agcgcaggac gagcgggccg tgtttcagct
      901 ccatcgagta gaggtggtaa acgacctcga gataaccggg ctgaaggcgt ttggttttct
      961 gctcgacgat tgtcggcggg ccaccggcgg gatcgggctc ggtggtcttg gtggtttcga
     1021 cgatttcttt ttctggccaa tcgatgccgg tgacgttcga gcaatagtcg aaacgaagtc
     1081 cggcggcgtc gcgaaggaag agcgagatgt cgcgagcgtg cgcgtggtcg agcagcagcg
     1141 aatgttcggc ggcggggccg gggtttgtgc tgagcgcgat ctgcgccgcg gggaaacggg
     1201 cgagaagacg atcgcgaatc tggtcgagcg tttccatggc ggcgcgctga cgcgctatcg
     1261 cgcgaaagct ccaagctcca agatccaagc tcccgagaag ctccaaacat cagagacagc
     1321 gggtttggag attgaaggtt ggaggttcat tggagcttgg tgcttggagt ttggagcttc
     1381 cggcgccggg gtttgattta cgctggcgat ttcgagctgc ggaccggcgc cggcggctgc
     1441 cagacatcgg gatttttcgg tgggacgaga tcgtgttcgc cgaattcagg aattggatac
     1501 tcgctcgg
//
LOCUS       DASPLT010000163         1730 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_43075, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000163 DASPLT010000000
VERSION     DASPLT010000163.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1730)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1730)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1730
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_43075"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..483)
                     /locus_tag="VEA63_01725"
     CDS             complement(<1..483)
                     /locus_tag="VEA63_01725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD82728.1"
                     /translation="MTGAQLLAYVKQRLGEAGLTWEGDRDGELYDALTEARDEIRQRL
                     AMSPSGSIAVRETVTLEAGDTERLYVVPDATAEPVRVLAVRVPGRSKPIAASQYEWVD
                     TRTVQLACGTGSSAGLELVAVLRIEDTIGAGTTEANIGLPTSAHRACGKLAAALALTV
                     N"
     gene            complement(480..854)
                     /locus_tag="VEA63_01730"
     CDS             complement(480..854)
                     /locus_tag="VEA63_01730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD82729.1"
                     /translation="MPLVIRQKMVTINTNNGKTPTDVTIYVEPKDVLPTDRVVKPTPR
                     QLRELAEQYPEYRGEAAKLQAEDGLSGKQFALLPWDKAVAVVDSTVDEKQLASWFAHE
                     IERQGGPRKSVLDEFAKKGYTQ"
     gene            complement(862..1404)
                     /locus_tag="VEA63_01735"
     CDS             complement(862..1404)
                     /locus_tag="VEA63_01735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD82730.1"
                     /translation="MIKIVVGLEDHVAPEPGPLELYQLTWPHRWVEWDRSRQLFKVMQ
                     TNPITGGDERYEILYDYDTPLDQEGAPIGGEALEKLLKEQPHAVQRRFRPFDYEFVRE
                     RRQQRFEFLHDGSRKYVEKTAREVTRIQRAIIRSRTSESAAALGEIRRWLPKMHGMEK
                     IPLVPGGFTTRANESVQKAS"
     gene            complement(1401..>1730)
                     /locus_tag="VEA63_01740"
     CDS             complement(1401..>1730)
                     /locus_tag="VEA63_01740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD82731.1"
                     /translation="AFTATTHDIAANASTVQERVYLYSLNAAGDVTVTPGVVATGAGN
                     AAFPATPAGQTPFGALRLAVAAGATPFDATTDELDEAHLTDTYYDLSVNPVDLVVTQE
                     ELEEIGA"
BASE COUNT          270 a          589 c          531 g          340 t
ORIGIN      
        1 gttcacggtg agtgcgagcg cggccgcgag cttcccacag gcgcggtgcg cggacgtcgg
       61 aagaccgata ttcgcctccg tcgtgccggc gccgatggtg tcttcgatgc gcagcactgc
      121 gacgagttcc agtccggccg acgatccggt accacatgcg agctgcacgg tgcgagtgtc
      181 gacccattca tactgcgacg cagcaatggg cttcgaccgt cccggcaccc gtactgcgag
      241 gacgcgcacc ggctccgcgg tcgcatcagg cacgacgtac agccgctcgg tgtcgcccgc
      301 ctccagcgtc accgtctcac ggacggcgat cgatccggac ggcgacatgg cgagccgctg
      361 gcgaatctcg tcacgcgctt ccgtgagcgc gtcgtagagt tcgccgtcgc ggtcgccttc
      421 ccatgtgagc cccgcctcac cgagccgttg cttcacgtac gcgagcagct gcgcaccggt
      481 cactgcgtgt agcccttctt tgcgaactca tccaggaccg acttacgcgg gccgccctgg
      541 cgttcgatct cgtgcgcgaa ccacgaggcg agctgcttct cgtcgacggt gctatcaacc
      601 accgccaccg ccttgtccca aggcaggagc gcgaactgct ttccgctgag tccgtcttcg
      661 gcctgcaact tcgcggcttc gccccggtac tccggatact gctcggcgag ttcgcgcagc
      721 tggcgcggcg tcggcttcac gacccggtct gtcggcagca catccttcgg ctcgacgtag
      781 atcgtgacgt ccgtgggagt cttcccgttg ttcgtgttga tcgtgaccat cttctggcgg
      841 ataacgagcg gcataatgtc ctcagcttgc tttctgaacg gattcgttcg cccgtgtggt
      901 gaatccaccg ggcacgagcg ggatcttctc cataccgtgc atcttcggaa gccagcggcg
      961 aatttcaccg agcgcggccg ccgactcgct ggtgcgcgaa cggataatgg cgcgctggat
     1021 ccgcgtgact tcgcgagccg tcttctcgac gtacttccgc gacccgtcat gcaggaactc
     1081 gaaccgctgc tgccggcgtt cccggacgaa ctcgtaatcg aagggtcgga agcggcgctg
     1141 cacggcgtgt ggctgctctt tcagcagctt ttccagcgct tcgccgccga tcggcgcgcc
     1201 ctcctgatcg agcggggtgt catagtcgta taggatttcg tatcgctcat cgccgcccgt
     1261 gataggattg gtctgcatca ccttgaacag ctggcgactg cgatcccatt ccacccaccg
     1321 atgcggccac gtgagctggt acagctcgag cgggccgggc tcgggcgcca catgatcctc
     1381 aagcccgacc acgatcttta tcaagcgccg atctcctcaa gttcctcctg cgtcaccacc
     1441 agatccaccg ggtttaccga gagatcgtag taggtgtccg tcaggtgagc ttcgtcgagc
     1501 tcgtcggtcg ttgcatcgaa cggcgtcgca ccggccgcca ccgcgaggcg gagcgcaccg
     1561 aacggcgtct gtcctgctgg cgtcgcaggg aacgcggcat tgccggcacc agtcgcaacc
     1621 acgccaggcg tcaccgtcac atcacccgcg gcgttcagcg agtacaggta gacgcgctcc
     1681 tgcacagtgc tggcgttcgc cgcgatgtcg tgtgtcgttg ccgtgaatgc
//
LOCUS       DASPLT010000164         1279 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_186332, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000164 DASPLT010000000
VERSION     DASPLT010000164.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1279)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1279)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1279
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_186332"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..536
                     /locus_tag="VEA63_01745"
     CDS             <1..536
                     /locus_tag="VEA63_01745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007361955.1"
                     /note="Members of this family of hydrolases with an active
                     site Cys residue belong to MEROPS family C26; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HYD82732.1"
                     /translation="KIAAARFARENKVPYYGLCLGLQIAVIEFSRNVLKLEGANSTEF
                     EASPRHPVINMMEEQKKVIDKGATMRLGSYECALTPGTRAAKAYAAESIRERHRHRYE
                     VNNAYVDQLKRGGMVVSGVNPRRNLVEIVELKDHPWFLAVQFHPEFQSKPNRAHPLFA
                     AFIAATIKNQKSTKKKR"
     gene            651..>1279
                     /gene="kdsA"
                     /locus_tag="VEA63_01750"
     CDS             651..>1279
                     /gene="kdsA"
                     /locus_tag="VEA63_01750"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007361954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HYD82733.1"
                     /translation="MIFDPDKLLVIAGPCSLENETVCRSVADTLAKLRAAHPELNLVF
                     KGSFDKANRTSLTGDRGTGLEDGLALLALINHEYDFPVLTDVHEREQVPAVAAVCDVL
                     QIPAFLCRQTDLLLAAAATGRVVNVKKGQFLSPQEMQFVTGKLRDGKAAEIWQTERGT
                     TFGYQNLVVDMRSFSIMRRNGFPTVFDATHSVQLPGAAGGKSGGQREFVP"
BASE COUNT          272 a          366 c          398 g          243 t
ORIGIN      
        1 gcaagatcgc cgcggcacga ttcgcacggg agaacaaggt gccttactat ggtctttgtc
       61 tcggcttgca gatcgcggtg atcgaatttt cgcgcaacgt gctcaaactc gaaggcgcga
      121 acagcacgga attcgaagcg agtcctcgtc atcccgtcat caacatgatg gaggagcaga
      181 aaaaagtgat cgataagggc gcgaccatgc ggctggggag ctacgagtgc gcgctcacgc
      241 cgggcacccg cgccgcaaag gcgtatgcgg cggagagcat tcgtgaacgc catcggcatc
      301 gctacgaggt gaacaatgct tacgttgatc aactgaaacg tggtggcatg gtggtcagcg
      361 gcgtgaaccc gcggcggaac ctcgtcgaga tcgtcgagct gaaggatcat ccgtggtttc
      421 tcgcggtgca gtttcacccc gagtttcagt cgaagccgaa ccgcgcgcat ccgctcttcg
      481 cggcgttcat cgcggcgacg atcaagaacc agaagagcac taaaaagaag cggtaaggcg
      541 aagcgatggc cacaaggggc gcaagagacg cagaaacgaa cggaaaatat tgcgcgcttt
      601 gagccttctg tggccgtgac ttcatcgatc ccgcggtagc attcgcctcc atgattttcg
      661 atcccgacaa acttctcgtc atcgccggtc cgtgttcctt ggagaacgaa acggtctgtc
      721 gcagcgtggc ggatacactc gcgaagttgc gcgctgcgca tcccgagctg aacctcgttt
      781 tcaaaggttc gtttgacaaa gccaaccgca cgtcgctcac cggcgatcgc ggcacgggat
      841 tggaggacgg tctcgcattg ctcgcgctca tcaatcacga gtatgacttc ccggttctga
      901 ccgatgtgca cgaacgcgag caggtgccgg cggtcgcagc ggtgtgcgat gtcttgcaga
      961 ttcccgcatt tctctgccgg cagacggatc tgcttctcgc cgccgcagcg acggggcgcg
     1021 tggtcaatgt gaagaaaggc cagttcctct cgccgcagga aatgcagttc gtcacgggca
     1081 aattgcgtga cggcaaggcg gccgaaatct ggcagacgga gcgcggcacg acgtttggct
     1141 accagaacct ggtcgtggac atgcgctcct tctcgatcat gcggcgcaat ggttttccca
     1201 ccgtgttcga tgcgacccac agcgtgcaat tgcccggtgc cgcgggcgga aaaagcggcg
     1261 ggcagcgcga attcgtgcc
//
LOCUS       DASPLT010000165         1485 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_207423, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000165 DASPLT010000000
VERSION     DASPLT010000165.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1485)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1485)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1485
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_207423"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..213
                     /locus_tag="VEA63_01755"
     CDS             <1..213
                     /locus_tag="VEA63_01755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD82734.1"
                     /translation="TYFRVNHAFKAVALPSAGTHRIAFTYWPQHFTRSLVLCAVGFAI
                     LATLLVALRISRPTALAAAGAPPASS"
     gene            242..>1485
                     /locus_tag="VEA63_01760"
     CDS             242..>1485
                     /locus_tag="VEA63_01760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD82735.1"
                     /translation="MSAASDGIGPHPRSGWVNRFALLIAALALWLASINLPGPPITNL
                     DSWHTILVDARLHDRQFGPEIAFTYGPWGFLSLSGYLPDALAEKFAWEVLGKLTLAIT
                     LVLATARVRGGRRWVLLAAFACGLPFFDNSVGVLIPLLALVWLLPRDAAPWQRLLAVL
                     WLALFAHFKFTFCLQALAALLIVTTTACIERRFRDAALVLLASTFAYVGFWIAAGQSL
                     ANLPAFCQLSWNLGSGYSWAMSSAPSTAALAAGIFTLAICAAFLRLIWHADLPLTQRL
                     AAAAIALANGFMAWKHGFTRGDHTFAFFFFCLFFGLLLAGLFSRRRGLVWHDLDVLGC
                     LAGIAIAGSGLLLHGPRTALDHWLRFPYELTHLDAWTERFRIALRQTENTAHDPALVA
                     AVGDATVDLINYDQWALLLNGL"
BASE COUNT          206 a          594 c          365 g          320 t
ORIGIN      
        1 acttactttc gcgtgaacca cgcgttcaaa gctgtcgccc ttccttctgc cggcacccac
       61 cgcatcgcct tcacctattg gccgcagcac ttcacccgct cgctcgtgct ctgcgcagtt
      121 ggcttcgcca tcctcgccac cctcctggta gcgctgcgta tctcccggcc gaccgctctc
      181 gccgcagccg gtgcacctcc ggcgagttca tgatgacaca gtccgccttg aaacgcggcc
      241 aatgagcgca gcatcggacg gcattggacc ccatcctcga tccggatggg taaaccgttt
      301 cgccctccta atcgccgcgc tcgccctctg gctcgccagc atcaaccttc ccggcccgcc
      361 gatcaccaac ctcgactcgt ggcacaccat cctcgtcgac gcccggctgc acgaccgtca
      421 gttcggtcca gagatcgcct tcacttacgg cccctggggg ttcctctctc ttagtggcta
      481 tctacccgac gcgctcgccg agaaattcgc ctgggaggtc ctcggcaaac tcactctcgc
      541 catcaccctc gtgctcgcca ccgcccgcgt tcgcggcggt cgacgctggg tgctcctcgc
      601 tgcgttcgcc tgcggccttc cgtttttcga caattccgtc ggcgttctca ttcctctgct
      661 ggccctcgtg tggttgctcc cgcgcgacgc cgcgccgtgg caacgcctgc tcgccgttct
      721 ctggctcgcg cttttcgccc acttcaaatt cacgttctgc cttcaagcgc tcgccgccct
      781 cctcatcgta acaacgaccg cttgcatcga acgccgtttc cgcgacgccg ccctcgtcct
      841 gctcgcttcc accttcgcct acgttggctt ctggatcgcc gccggccaat cgctcgcgaa
      901 cctccccgca ttctgtcaac tcagttggaa tctcggcagc ggctactcgt gggccatgtc
      961 gtcagcgccc agcaccgccg ccctcgccgc cggcattttc accctcgcga tctgcgccgc
     1021 atttctccgg ctgatttggc acgcagactt gccgctcaca caacgcctcg cagccgccgc
     1081 catcgcactc gccaacgggt tcatggcttg gaaacacggc ttcacccgcg gagaccacac
     1141 gttcgccttt ttctttttct gcctgttctt cggtcttctc ctcgccggac tgttctcgcg
     1201 acgccgcgga ctcgtctggc acgacctcga cgtccttggc tgcctcgccg gcatcgcgat
     1261 cgccggcagc gggctcctgc tccacggacc gcgcaccgcg ttggatcact ggctccgctt
     1321 tccttacgaa ctcacgcacc tcgacgcctg gaccgaacgc tttcgcatcg cgctccgaca
     1381 aacggaaaac accgctcacg atccggcact cgtcgccgcc gtgggcgatg cgaccgtcga
     1441 cctcatcaac tacgaccagt gggcgcttct gctcaacggc ctcaa
//
LOCUS       DASPLT010000166         1475 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_289520, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000166 DASPLT010000000
VERSION     DASPLT010000166.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1475)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1475)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1475
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_289520"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..688)
                     /locus_tag="VEA63_01765"
     CDS             complement(<1..688)
                     /locus_tag="VEA63_01765"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HYD82736.1"
                     /translation="MRILHLEDSPPDAELVAARLTSDFPDCRIKHAANREQFEHALGG
                     GDFDLILSDYTLQNFDGLTALDIARSRCPSKPFIFLSGTIGEERAIEALRRGATDYVI
                     KDRPSRLISAIRGAIARADEERQLRETEGALRENQERFRQIAENVADLILMLDLSGRC
                     VYCNPAYAHAMGRDPSAAHDDPFVHVHAEDHEAVRQAFRDTARDGQSRRLEYRLVRPD
                     GTVRFVEAQNT"
     gene            complement(714..1157)
                     /locus_tag="VEA63_01770"
     CDS             complement(714..1157)
                     /locus_tag="VEA63_01770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HYD82737.1"
                     /translation="MDAPARILLAEDSPQDVEMTLAAFAEHKLSNEIVVVNDGEQALD
                     YLFSRGRFENRASGNPILVLLDLKMPKVDGLEVLRQIKADETLRTTPVVMLTSSREEQ
                     DIVRSYRLGINAYVVKPVDFHAFIEAVKQLGTFWTIHNEPPPSAH"
BASE COUNT          265 a          376 c          557 g          277 t
ORIGIN      
        1 cggtgttttg cgcttcgaca aaacgcaccg tgccgtcggg gcgaacgagt cggtattcga
       61 gacggcggct ctggccatcg cgcgcggtgt cgcggaaagc ctgacgcacg gcttcgtggt
      121 cctcggcgtg aacgtggacg aaaggatcgt cgtgcgcggc ggacggatcg cggcccatcg
      181 cgtgcgcgta ggcagggttg caataaacgc agcggccgga gagatcgagc atcaggatca
      241 ggtcggcgac gttctcggcg atctggcgga aacgttcctg attttcgcgc agcgcaccct
      301 cggtttcgcg gagctggcgt tcctcgtcgg cgcgggcaat ggcgccgcgg atcgcggaga
      361 tgaggcgcga cggacggtct ttgatgacgt agtcggtggc gccgcgtcgg agggcctcga
      421 tcgcgcgttc ttcgccgatc gtgccggaga gaaagataaa cggtttcgac gggcagcggg
      481 agcgggcgat gtcgagcgcg gtgaggccgt cgaaattctg cagcgtataa tcggagagga
      541 tcaggtcgaa atcgccgccg ccgagcgcgt gctcgaactg ttcgcggttg gccgcgtgtt
      601 tgatgcggca atcgggaaaa tcggacgtga gacgggcggc gacgagctcg gcgtcgggcg
      661 gggaatcttc aaggtgcagg atgcgcatgg cgggggcgac gggcgcgaga aggtcaatgg
      721 gcggaaggcg gcggctcgtt gtggatggtc cagaacgtgc cgagttgttt caccgcttcg
      781 atgaaggcgt gaaagtcgac gggcttgacg acgtaggcgt tgatgccaag ccggtagctg
      841 cgaacgatgt cctgctcttc gcgagaggac gtgagcatca cgacgggcgt ggtgcggaga
      901 gtctcatccg ccttgatctg gcggagcacc tcaaggccat cgaccttcgg catcttgagg
      961 tcgaggagca cgaggatggg attgcccgac gcgcggtttt caaagcggcc gcgggagaag
     1021 aggtagtcca gcgcctgctc gccgtcgttc accaccacga tttcgttgga aagcttgtgc
     1081 tccgcgaagg cggcgagcgt catttcaaca tcttgggggc tgtcttcggc gaggagaatg
     1141 cgggcgggag cgtccatgag cgggaaaagg agtgatgaag agcgtcggaa gcgaagggag
     1201 acgtctccgc gacagttcat cggaagagtc cccggggaaa agagcgggtt cttcttttcg
     1261 aggcaattcg ccagaatttt acgtatgaac gcaatcagtg gatgatggga gcggcgaggt
     1321 gacttgaggt tcgggggagg cggcgccaag gtgtcggttc gcatgcgaat cggtcctcac
     1381 gcgctttcct cgaacctgtt tctgtcgccg ttggcgggtt acacgaacct gccgtttcgg
     1441 ctgacgttgc gggagctggg cggcttgggc tgggc
//
LOCUS       DASPLT010000167         1240 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_166095, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000167 DASPLT010000000
VERSION     DASPLT010000167.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1240)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1240)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1240
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_166095"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1240
                     /locus_tag="VEA63_01775"
     CDS             <1..>1240
                     /locus_tag="VEA63_01775"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HYD82738.1"
                     /translation="KEDGKEGSYVIRLLNTTGQPALTSLTNRAVRERLMAASLARGSS
                     GGEFDNRALITRLTQLRAQRASLLGFANYAAYQLSEQTAGDVATVNKLLADLAKPAVA
                     NARREAADMQEIIDRENGGFTLAAHDWAFYSEKVRQARYAFDENQLKPYFELEHVLVD
                     GVFFAATKLYGITFKERHDLPVYQEDVRVFEVFDTDGAPLGLFLFDPYARPSKRGGAW
                     MNEYVSQSGLFGRKPVVANHLNIPKPPASEPTLLTFDEVTTAFHEFGHALHGLFSNVH
                     FPRFSGTNVPRDFVEFPSQVNEMWATWPEVLANYAKHHQTGEPMPAELLAKVLDARKF
                     NQGHKTTEYLAASLLDQAWHQLKPDAVPTADNALAFEASALKKAGVDFPPVPPRYRST
                     YFSHIFASDGYSAGYYSYIWS"
BASE COUNT          249 a          490 c          300 g          201 t
ORIGIN      
        1 aaaagaagac ggcaaggaag gcagctacgt catccggctc ctcaacacca ccggccagcc
       61 ggctctcaca tcgctcacga accgcgccgt ccgcgaacgc ctcatggccg cctcgctcgc
      121 ccgcggcagc agcggcggcg agttcgacaa ccgagctctg atcacgcgcc tgactcaact
      181 ccgcgcccaa cgtgcttcgc tcctcggctt cgccaactac gccgcttacc aactctccga
      241 gcagaccgcc ggcgacgtcg ccaccgtcaa caaactcctc gccgacctcg cgaaacccgc
      301 cgtcgccaac gcccgccgcg aagccgccga catgcaggaa atcatcgatc gcgaaaacgg
      361 cggcttcacc ctcgccgccc acgactgggc gttctactcc gagaaagtcc gccaggctcg
      421 ctacgccttc gacgagaacc agctcaaacc ctacttcgaa ctcgaacacg tgctcgtcga
      481 cggcgtcttc tttgccgcca cgaaactcta cggcatcacc ttcaaggaac gtcacgatct
      541 ccccgtttat caggaagacg ttcgcgtgtt cgaagtcttc gacaccgacg gcgcgccgct
      601 tggccttttc ctcttcgacc cctacgcgcg cccgtcaaaa cgcggcggcg cgtggatgaa
      661 cgaatacgtt tcccagtccg gcctcttcgg ccgcaagccc gtcgtcgcca atcatctcaa
      721 catcccgaag ccacccgcaa gcgaaccgac gctcctcacc ttcgacgagg tcacgaccgc
      781 cttccacgaa ttcggccacg cgctccacgg cctcttctcc aacgtccact tcccgcgctt
      841 cagcggcacc aacgtgccgc gtgacttcgt cgagtttccc tcccaagtga acgagatgtg
      901 ggcgacctgg cccgaagtgt tggccaacta cgctaagcac caccaaaccg gcgagccgat
      961 gcccgccgaa ctgctcgcga aggtcctcga cgcccgcaaa ttcaaccagg gccacaaaac
     1021 caccgaatac ctcgccgcct cgctcctcga ccaagcctgg caccagctca agcccgacgc
     1081 cgtgccgacc gccgacaacg cgctcgcgtt cgaagcgtcc gcgctgaaaa aagccggcgt
     1141 cgattttccg cccgtgccgc cgcgttatcg cagcacgtat ttctcgcaca tcttcgcgag
     1201 cgacggctac tccgccggct attactccta catctggagc
//
LOCUS       DASPLT010000168         1134 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_43117, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000168 DASPLT010000000
VERSION     DASPLT010000168.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1134)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1134)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1134
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_43117"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..392
                     /locus_tag="VEA63_01780"
     CDS             <1..392
                     /locus_tag="VEA63_01780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HYD82739.1"
                     /translation="IEAPFIFRKGDDYFLFVSWDKCCRGAESTYKVVVGRSKSLTGPY
                     VDRTGKSLAEGGGTLLLEGNAAWAGVGHNSVYTFEGKDWLVFHAYETADNGRQKLKVA
                     ELTWDSAGWPQVDPRVLSEYVSELDEN"
     gene            521..>1134
                     /locus_tag="VEA63_01785"
     CDS             521..>1134
                     /locus_tag="VEA63_01785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HYD82740.1"
                     /translation="MLEKIGFGAGDMAVNVVWSSMALIITFFYTDVFGLRPSDLAALF
                     LTVRFIDAITDPLMGLITDKFTTRWGRYRPYFLWLAVPFGVSIFLTFVTPDLGYGGKL
                     MWAYATYILLTLIFTAVTIPYISLIGVLTDSPQERLSANGYRLFFAKVAAFLVTIIVP
                     VMAANWGGGSIARGYQIAMGVMAIAAAALFLFCFFATTERLQHEV"
BASE COUNT          206 a          278 c          391 g          259 t
ORIGIN      
        1 agatcgaggc gccgttcatt tttaggaaag gcgacgacta ctttttattc gtgtcgtggg
       61 acaaatgctg ccgcggcgcg gagagcactt acaaggtggt cgtggggcgt tcgaagagcc
      121 tcaccggccc gtatgtcgat cgcaccggaa agagtctcgc ggagggaggc ggcacgttgt
      181 tgctcgaggg aaatgcggca tgggcgggcg tggggcacaa cagcgtctac acgtttgaag
      241 gaaaggattg gctggtgttt cacgcctacg agacagcgga caacggtcgg cagaagctga
      301 aggtcgcgga actcacgtgg gacagcgctg ggtggccgca agtcgatccg cgggtgctga
      361 gcgaatatgt gagcgaactc gatgagaact gatgtccgac tccgtggctc tacgcgtgag
      421 cgcagggacg aatggagcct cgccaatctc ccgctgctca cgcaacgggc tacgaaccat
      481 tgaaagcgtg aacatgaaat ccccaccgca aagactttcc gtgctcgaga agatcggctt
      541 cggcgcgggc gacatggcgg tgaacgtcgt gtggtcgtcg atggcgctga tcatcacgtt
      601 tttttatacg gacgtgttcg gattgcggcc gtcggatctc gcggcgctgt ttctcacggt
      661 gcggttcatc gacgccatca ccgacccgct gatggggtta attacggaca agttcacgac
      721 gcgctggggg cgttatcgtc catattttct gtggctggcc gtgccgttcg gggtgtcgat
      781 cttcctgacg tttgtgactc cagaccttgg atacggcggg aagctgatgt gggcgtatgc
      841 gacgtatatt ttgctcacgt tgattttcac cgcggtgacg attccctaca tctcgctgat
      901 cggcgtgctg acggattcgc cgcaggaacg gctgtcggcg aatgggtatc gattgttctt
      961 cgcgaaggtg gccgcgtttt tggtgacgat tatcgtgccg gtgatggcgg cgaattgggg
     1021 cggagggagc atcgcgcgcg ggtatcagat tgcgatgggc gtgatggcga tcgcggcggc
     1081 ggcgttgttc ttgttttgtt ttttcgcgac gacggagcgg ttgcagcacg aggt
//
LOCUS       DASPLT010000169         1801 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_83996, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000169 DASPLT010000000
VERSION     DASPLT010000169.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1801)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1801)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1801
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_83996"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..819)
                     /locus_tag="VEA63_01790"
     CDS             complement(<1..819)
                     /locus_tag="VEA63_01790"
                     /inference="COORDINATES: protein motif:HMM:NF024644.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HYD82741.1"
                     /translation="MVASRHNFPLLEELLAEQQRLATPVAKFSDVHDRSSAPALAAVY
                     RDLIPLSAPKPGEQYAFEVELDSCTGCKACVSACHSLNGLDETETWRDVGLVVGGDWR
                     ESFQQTVTTACHHCADPACLNGCPVLAYEKDPVTGIVRHLDDQCIGCQYCVLKCPYDV
                     PKYNARLGIVRKCDMCHSRLADGEAPACVQACPTRAIRIVTVAVAPATTAAAMTATAA
                     ENQSVTQCVTLSGSTPALDAMTSAFLKSAPSPDYTQPTTRYVSRRTQPADLHAAD"
     gene            complement(858..>1801)
                     /locus_tag="VEA63_01795"
     CDS             complement(858..>1801)
                     /locus_tag="VEA63_01795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007363616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sulfite reductase subunit alpha"
                     /protein_id="HYD82742.1"
                     /translation="ALGCDGEEAVPALDGSTTSLRRALTEVYDLGKPTPELLAIVGGA
                     LRCDSDGGSVMSGHKAPPTVTTGAYHHVIDVLLAAPEAKPPAVEFVRALKKLQPRLYS
                     ISSSPKAHPGQVHLTVGAVRYDKDGRARKGVCSTFLAERAPTGVARIGVFVHSNKAFR
                     PPANGDAPMIMVGPGTGIAPFRAFLEERRAAGAKGKNWLFFGDQRAATDFLYREELDE
                     LAHAGVLTRLDTAFSRDQAEKIYVQHRMLENAAELYAWLEQGAHFYVCGDASRMAKDV
                     DAALHQVIELAGRKTAEQAADYVQKLKATKRYARDVY"
BASE COUNT          329 a          625 c          528 g          319 t
ORIGIN      
        1 atccgccgcg tgcaaatccg ccggctgcgt tcgccgagaa acataacgcg tcgtcggctg
       61 cgtgtaatcc ggcgacggcg cacttttcaa aaacgccgac gtcatggcgt ccagcgccgg
      121 cgtggaaccg gacagtgtca cgcattgcgt gacactttgg ttttctgccg cagtcgcggt
      181 catcgccgct gccgtcgtcg caggcgcaac cgccaccgtc acgattcgaa tcgcgcgcgt
      241 cggacacgcc tgcacgcacg ccggcgcttc accgtccgcg agccggctgt ggcacatgtc
      301 gcacttccgc acgatgccga gacgcgcgtt gtatttggga acgtcatacg ggcatttcag
      361 cacgcaatat tggcagccga tgcactggtc atccagatgc cgcacgatgc cggtgaccgg
      421 gtccttttca taagcgagca ccgggcagcc gttgagacac gccggatcgg cgcagtgatg
      481 acacgccgtc gtcaccgttt gctggaacga ctcacgccaa tcgccgccga ccaccaaccc
      541 gacgtcgcgc cacgtttccg tctcgtcgag accgttcagc gagtgacacg ccgacacgca
      601 cgccttgcaa ccggtgcacg aatcgagctc gacttcgaac gcgtattgct cgccgggttt
      661 cggcgcgctc agcggaatca agtcgcggta aaccgccgcg agcgccggcg ccgaagacct
      721 gtcgtgcacg tccgaaaact tcgcgaccgg cgtggcgagc cgttgctgct ccgcgagaag
      781 ttcctcgagc agcggaaagt tgtgccgtga agcaaccatg tcagccgcgc ctcgtgcgca
      841 ggttcgggca gcgatgttta atacacatcg cgcgcataac gcttcgtcgc cttcagcttc
      901 tgcacgtaat ccgccgcctg ctcggcggtc ttgcggccgg cgagctcgat cacttggtgc
      961 agcgccgcgt cgacgtcctt cgccatccgg ctcgcatcgc cacacacgta gaaatgcgcg
     1021 ccctgttcga gccacgcgta gagctccgca gcgttctcga gcatgcgatg ctgcacgtaa
     1081 attttctccg cctgatcgcg cgagaacgcc gtgtcgagtc gcgttagcac gcccgcgtgc
     1141 gccaattcgt caagttcttc gcgatagaga aaatccgtcg ccgcgcgttg gtcgccgaag
     1201 aacaaccagt tcttcccttt cgcacccgcc gcacgacgct cctcgagaaa cgcgcgaaac
     1261 ggcgcgatac ccgtgcccgg acccaccatg atcatcggcg cgtcgccatt cgccggcgga
     1321 cgaaacgctt tgttcgaatg cacaaacacc ccgatgcgcg ccacgcccgt cggcgcgcgt
     1381 tccgcgagaa acgtggaaca cacgcctttg cgcgcgcggc cgtctttgtc gtagcgcacc
     1441 gcacccaccg tgagatgcac ctgccccgga tgcgccttgg gcgaagagga aatcgaatag
     1501 agccgcggct gaagtttctt caacgcgcga acaaactcga cggccggcgg ctttgcctcg
     1561 ggcgcggcga gcagcacatc gatcacgtga tgatacgcac cggtcgtcac tgtgggaggg
     1621 gctttatgcc ccgacattac actcccgcca tctgagtcgc agcgtaaagc tccgcccacg
     1681 atggccaaca actccggcgt cggttttccg agatcgtaaa cttccgtgag cgcgcgccgc
     1741 agcgacgtcg tgctgccatc cagcgccggc accgcttcct cgccatcgca gccgagcgcg
     1801 g
//
LOCUS       DASPLT010000170         1328 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_84001, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000170 DASPLT010000000
VERSION     DASPLT010000170.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1328)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1328)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1328
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_84001"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..442
                     /locus_tag="VEA63_01800"
     CDS             <1..442
                     /locus_tag="VEA63_01800"
                     /inference="COORDINATES: protein motif:HMM:NF013227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HYD82743.1"
                     /translation="THIRLGRRLLDHLRELALPGIVLPAIDPRDTHSFWFCLLRLETE
                     KFHCTRNEFVAALEAEGVDARAGYIPKPVYKYPLFQHHNFFAGAWPLRDAGLTTMDYR
                     TVVCPVAEAMLADGVSLPITPALTDETIARIAEALAKVARHFAR"
     gene            complement(657..>1328)
                     /locus_tag="VEA63_01805"
     CDS             complement(657..>1328)
                     /locus_tag="VEA63_01805"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HYD82744.1"
                     /translation="LRRHGTRRALVSPLEAVFAPEPGPANRALLESTRGVGRLEPVPV
                     INPALANAHEELARCAADERVRAVRLLPNYHNFGLRARAVETLIGELKNRGLRLIVQM
                     QLIDQRHEYHAMNLKPVPPDDLAILLERHPKWSVMASGLMRPEILRLAPKFPRLLADL
                     SFAEWHDTMENLLAKVSVRQLVFASHTPLLITAAARAKLETSTVGEGKRRAITGENLE
                     RFLAE"
BASE COUNT          267 a          512 c          297 g          252 t
ORIGIN      
        1 cacccacatc cgcctcggcc gccgcctcct cgaccacctg cgcgaactcg ccctccccgg
       61 catcgttctt cccgcgatcg atccccgcga cacccacagc ttctggttct gcctcctccg
      121 cctcgaaacc gaaaaattcc actgcacccg caacgaattc gtcgccgcgc tcgaagccga
      181 gggcgtcgat gcccgcgccg gctacatccc aaaacccgtc tacaagtatc cacttttcca
      241 gcaccacaat ttcttcgccg gcgcctggcc gctccgcgac gccggcctca ctacgatgga
      301 ctatcgcacc gtcgtctgcc ccgtcgccga agcgatgctc gccgacggcg tttccctgcc
      361 gatcaccccc gcgctcaccg acgagaccat cgcccgaatc gccgaagccc tcgccaaagt
      421 agcccgccac ttcgcccgct gagttcagaa attaaccacg aatggacacg aattcacacg
      481 aataaaaccg ggaccaagcg ccaccgattc cattcgtgtc tattcgtgta tattcgtggt
      541 tttaaagttc cgatccgttg aaccacggat aaacacggat gcacacagat aaaacaatcc
      601 ctctccatcc gaatccgtgt tcatctgtgt tcatccgtgg ttcaaaacat tcaccatcac
      661 tccgccagaa accgctccaa attttctccc gtaatcgccc gccttttccc ttcgcccacc
      721 gtcgacgtct caagcttcgc ccgtgccgcg gccgtgatca gtagcggcgt atgcgacgcg
      781 aacaccagtt gtcgcaccga caccttcgcg agcaaattct ccatcgtgtc gtgccattcc
      841 gcgaatgaca gatctgcgag caaccgagga aacttcggcg ccaatcgtaa aatctccggc
      901 cgcatcaacc cgctcgccat caccgaccat ttcggatgcc gctcgagcaa tatcgccaaa
      961 tcgtctggcg gcaccggctt caaattcatc gcgtgatact cgtgccgctg atcgatcaac
     1021 tgcatctgca cgatcaaccg cagtccgcga tttttcagct ccccgatcaa cgtctccacc
     1081 gcgcgtgccc taaggccgaa gttgtgatag ttcggtagca gccgcaccgc gcgcacgcgc
     1141 tcatccgctg cacaccgcgc cagttcctcg tgcgcgttcg ccagcgccgg attgatcacc
     1201 ggcaccggct ccaatctccc cacgccgcgc gtgctctcga gcaacgcccg attcgccggc
     1261 cccggctccg gcgcgaacac cgcctcgagc ggcgacacca gcgcacgccg agttccatgt
     1321 ctccgcaa
//
LOCUS       DASPLT010000171         1423 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_166133, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000171 DASPLT010000000
VERSION     DASPLT010000171.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1423)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1423)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1423
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_166133"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..484
                     /locus_tag="VEA63_01810"
     CDS             <1..484
                     /locus_tag="VEA63_01810"
                     /inference="COORDINATES: protein motif:HMM:NF024762.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HYD82745.1"
                     /translation="EQCRVINRVLFHEWGFHGNVENYTDPLNSLLDHVLARRKGIPIS
                     LSTTYLLIAERLGLELEPVGLPGHFIVGCFTEAKPFFIDPFDRGIFLDTDEVFALLRA
                     NKIVPKSTDLMPTPIREVLCRSCRNLVNHYKAAGDDDHAKLFASFVDEFDSAYEKNSA
                     "
     gene            572..>1423
                     /locus_tag="VEA63_01815"
     CDS             572..>1423
                     /locus_tag="VEA63_01815"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009511547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HYD82746.1"
                     /translation="MAASDPVLTLRDLESWSFDGTALAVLGQPIKHSISPAMHNAALA
                     EMARTDPRFASWKYFRFEIAPADLPHALPLLHEKKFRGINLTVPHKILAVEHVAAIDP
                     AAQIAGAVNTLLSTAHGWHGHNTDGYGLANGLHQDLGLSLSGAHVILLGAGGAARGAA
                     VECLRQRCASLSIANRTRENLDALLSLLQPLAGDTSLRGFSPQTPPADLPANAIVINA
                     TSAGLRDTDPLPIALETLPRASGVYDMIYNPPRTALLRVAATLEVPHANGLSMLVHQG
                     AKSLAIWS"
BASE COUNT          252 a          557 c          355 g          259 t
ORIGIN      
        1 cgaacaatgc cgcgtcatca accgcgtgct gtttcacgaa tggggctttc acggcaacgt
       61 cgagaactac accgatccgc tcaacagcct gctcgatcac gtcctcgccc gccgcaaggg
      121 catcccgatt tccctcagca cgacctacct gctgatcgcc gaacgcctcg gcctcgagct
      181 cgagcccgtc ggcctgcccg gtcacttcat cgtcggctgc ttcacggaag cgaagccgtt
      241 tttcatcgac ccgttcgacc gtgggatttt tctggataca gacgaagtct tcgccctcct
      301 ccgcgccaac aagatcgtgc cgaaaagcac cgacctgatg ccgacgccga tccgcgaagt
      361 cctctgccgc agctgccgca atctcgtcaa ccactacaag gccgccggcg acgacgacca
      421 tgcgaagctc ttcgcgagct tcgtcgacga gttcgattcc gcctacgaga aaaacagcgc
      481 gtagaatcgt tctcgttctt cgttctcttt ctcgttctcc cgaaatcgcc ctcgctctcc
      541 gagaacgaga acgagtaaga gaacgagaac gatggctgct tccgaccccg tcctcaccct
      601 ccgcgatctc gaatcgtggt ccttcgacgg caccgcgctc gccgtcctcg gccagccgat
      661 caagcactcg atcagcccgg cgatgcacaa cgccgcgctc gccgagatgg cgcgcaccga
      721 tccgcgtttt gcttcgtgga aatatttccg cttcgagatc gcgcccgccg atctcccgca
      781 cgcgctgccg ctgctccacg agaagaaatt tcgcggcatc aatctcaccg ttccgcacaa
      841 gatcctcgcg gtcgaacacg tcgccgcgat cgatcccgcc gcgcaaatcg ccggcgccgt
      901 caacacgctc ctctccaccg cgcacggctg gcacggccat aataccgacg gctacgggct
      961 cgcgaacggc cttcatcaag atctcggcct ctctctctcc ggcgcccacg tcattctcct
     1021 cggtgccggc ggtgccgccc gcggcgccgc ggtcgaatgc ctccgccagc gctgcgcgtc
     1081 gctctccatc gccaaccgca cgcgcgaaaa cctcgacgct cttctctcgc tcctgcagcc
     1141 gctcgccggt gacacctcgc tgcgcggttt ctcgccgcaa actccgcccg ccgatctgcc
     1201 ggcaaacgcc atcgtcatca acgccaccag cgccggtctg cgcgacaccg atccgctacc
     1261 catcgcacta gaaacgcttc cgcgcgcctc cggcgtttac gacatgatct acaatccgcc
     1321 gcgcaccgcg ctcctccgcg tcgccgccac gctcgaagtt ccccacgcca acggtctctc
     1381 gatgctcgtg caccaaggcg cgaagtcgct cgccatctgg agc
//
LOCUS       DASPLT010000172         2545 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_43165, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000172 DASPLT010000000
VERSION     DASPLT010000172.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 2545)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2545)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2545
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_43165"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..798)
                     /locus_tag="VEA63_01820"
     CDS             complement(<1..798)
                     /locus_tag="VEA63_01820"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HYD82747.1"
                     /translation="MHSPHRLSRREFVGQLSLAAAAAVTLPRGLRAAASPAGKKVGVA
                     LLGLGNYAESQLATALQVTQACRLVGAISGTPAKLDAWKTKFNLSADNLYGYDQLERV
                     ADNPEIDVVYVVTPPGTHRDFVVRAAKAGKHVICEKPMAPTVRECEEMIAACRGAGKK
                     LSIGYRLEFEPHHIEMERVAQGFGAAKMTGAFGFHMRDGRPWRVQKALAGGGSLWDLG
                     VYVIQAACRAAGDKLPVAVTAQNGPNTRPEMFNEVEESIRWTMEFADG"
     gene            complement(1232..>2545)
                     /locus_tag="VEA63_01825"
     CDS             complement(1232..>2545)
                     /locus_tag="VEA63_01825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD82748.1"
                     /translation="VECGLQIPADENAKPVYVEYDGDLFWVNINRRGELWINTREKPR
                     PVFRVVQAARIETEAAEEPKAENGGRRGEDRNQRSEERGQRSEDRGRRSEDRRQSDDE
                     PKTEAVRRSSGETAAMQPELPEVAAAETQSAKPGVSGGAAALPQGPALLERIRPLMRR
                     NRRGPGGSGSASFLARALKCSEADLSAAFTSLGLVVPATPADKPVDVEIAGDIWWLNK
                     DSRGGLWINGREKREGEAAKTSAATQDAGAGAGAGAEKAAAAAETSTGGATPGATDAP
                     SSEPGALPLESAASGASATADTASRSEPANVLGAIRLLLKETRTGSMAGKLERVAEEL
                     GKSPDDLTAALVGAGLKVPEKAREKPVFVEHAGEIFWFNRNAKGELWVNAKASKFANE
                     AEGGESGAEGEAAKKPSRRAPRSRNRAPKSGETPTSTDGDAATAE"
BASE COUNT          445 a          974 c          639 g          487 t
ORIGIN      
        1 accgtccgca aactccatcg tccaacgaat cgattcctcc acttcgttga acatctccgg
       61 ccgcgtgttc ggaccatttt gcgccgtcac cgccaccgga agtttgtccc ccgccgcgcg
      121 acatgccgcc tgaatcacat atacgcccag atcccacagc gacccgccgc ccgccagcgc
      181 cttctgcacg cgccacggac gtccgtcgcg catgtgaaac ccaaacgcgc cggtcatctt
      241 cgccgcgcca aatccctgtg cgactcgctc catctcgata tggtgcggct cgaactccag
      301 ccgatagcca atcgacaatt tctttcccgc cccgcgacac gccgcgatca tctcctcgca
      361 ctcgcgcacc gtcggcgcca tcggtttttc gcagatcaca tgcttccccg ctttcgccgc
      421 gcgcaccacg aaatcccggt gcgtgcccgg cggcgtcacc acgtaaacga catcgatctc
      481 gggattatcg gccacgcgct cgagctggtc gtaaccgtaa agattgtccg ccgacagatt
      541 gaacttcgtt ttccacgcat cgagcttcgc cggcgttccg ctgatcgcgc ccaccagccg
      601 gcacgcctgc gtcacctgca acgccgtcgc caattgcgac tccgcgtaat tgccgagccc
      661 cagcaatgcg acgcccacct tcttccccgc cggtgacgcc gccgcccgca acccgcgggg
      721 caatgtcacc gccgccgctg cggcgagcga aagttgtcca acaaattcac gacgcgagag
      781 acggtggggt gagtgcataa caacggagtt ggggttttaa gaaaacgctc gcacgtaaaa
      841 accccgcccg cgcgttggca aacccgcgca ccgaaaaacc ctgtcatcgc aacgcgaaca
      901 gccaacccgc ataaactaca cttcaacgaa ccaaacggtg ggaacacagc gaatacagag
      961 gtccgacata gagcccacaa agagagaaat acttgataca aacccgagcc ttcatcgtca
     1021 gcattcgcct gcatcaaaga cctccgaaca ccttgtggcc tcctgcttcc tccgtgtcgg
     1081 cgctctgtga tctctgtgtc acatgccttg attgattgga gtataactca cccaagcgag
     1141 aacccggaca tcccgtcgga ctcgatcgca ttgcgcccct cccgccgcga acacagcggg
     1201 aaggtcgcct ctaccttgaa agcgccgcgc gttactccgc agtcgcagcg tctccgtccg
     1261 tcgaggtcgg cgtctcaccg ctcttcggcg cgcgattgcg actgcgcggc gcacgccgcg
     1321 agggtttctt cgccgcttcg ccttccgcac cgctctcgcc gccttcggct tcgttcgcga
     1381 acttcgacgc tttcgcgttc acccacagtt cgcctttggc gttgcgattg aaccagaaaa
     1441 tttcgcccgc atgttcgacg aagaccggtt tttcgcgcgc tttctccggc actttcaaac
     1501 ccgcaccgac cagcgccgcg gtcaaatcgt caggcgactt cccgagctcc tcagcaacgc
     1561 gctcgagttt gcccgccatg gagcccgtgc gtgtttcctt caacaacagc cggatcgcgc
     1621 ccaacacatt cgccggctcc gaccgtgaag ccgtgtccgc cgtcgcactc gcgccactcg
     1681 ccgccgactc gagcggcaat gcgcccggtt ccgaagacgg tgcgtccgtc gcaccaggcg
     1741 tagcgccacc cgtcgacgtt tccgccgcag ccgcggcttt ttccgcgccg gcaccggcgc
     1801 ccgcgccagc gtcctgcgtc gcagccgacg ttttcgctgc ctcgccttcg cgcttctcgc
     1861 gtccgttgat ccaaagtccg ccgcgcgaat ccttgttaag ccaccaaata tcgcccgcga
     1921 tttccacatc caccggcttg tcggccggcg tcgccggcac caccaaaccg agcgacgtaa
     1981 acgccgcgct caaatccgct tcgctgcact tcagcgctcg tgccagaaaa ctcgcgctgc
     2041 ccgatccacc cggcccgcga cgattccgcc gcatcaacgg acggattctt tccaacaacg
     2101 ccggtccttg cggcaacgct gccgcaccgc ccgaaacacc cggcttcgcg ctctgcgttt
     2161 ccgctgccgc gacctcgggc aattccggtt gcatcgcggc ggtctcgccg ctcgaacgcc
     2221 gcaccgcttc ggtcttcggc tcgtcatccg actgtcttct gtcctccgac cgccggcctc
     2281 tgtcttccga cctctgacct ctttcctctg accgctggtt cctgtcctcg ccccgacgtc
     2341 cgccgttctc ggccttcggc tcttccgccg cttcggtctc gatgcgcgcc gcctgaacca
     2401 cccggaacac cggacgcggt ttctcacgcg tgttgatcca caactcgccg cggcggttga
     2461 tgttcaccca gaaaagatcg ccgtcgtatt cgacgtaaac cggcttcgcg ttttcgtcgg
     2521 cgggaatctg caaaccgcac tccac
//
LOCUS       DASPLT010000173         1390 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_2556, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000173 DASPLT010000000
VERSION     DASPLT010000173.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1390)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1390)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1390
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2556"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            39..>1390
                     /locus_tag="VEA63_01830"
     CDS             39..>1390
                     /locus_tag="VEA63_01830"
                     /inference="COORDINATES: protein motif:HMM:NF020920.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase family protein"
                     /protein_id="HYD82749.1"
                     /translation="MAKPANVKLLESWLRSYRPHELFDQNGRLRADLRELAPKGPRRM
                     SANPHANGGILLRDLRLPDFHDYAVKVPQPGATLAQATRVQGAFIRDVIKLNPDNFRV
                     FSPDETNSNRWNAVFEVTNRCSTAEIIPGDDHVAPDGRVMEVLSEHQCEGWLEGYLLT
                     GRHGFFSCYEAFIHIVDSMFNQHAKWLKVTRNLPWRRPIASLNYLLTSHVWRQDHNGF
                     THQDPGFIDHVVNKKAEIIRVYLPPDANTLLSVTDHCLRSRHYVNVIVAGKQPEAQWL
                     DMDAAVKHCTAGIGIWTWASNDEGGEPDVVMACCGDVPTLETLAAVDLLRQHFPRLKV
                     RVINVVDLMKLQSRREHPHGLSDAEFDGLFTTGKPVIFAYHGYPALIHRLTYRRTNHA
                     NIHVRGYKEEGTTTTPFDMVVLNELDRFHLAMEVIERVSQLGPRAAYDRQFFRDKVIE
                     "
BASE COUNT          282 a          467 c          379 g          262 t
ORIGIN      
        1 aaggcacgtt ccgctcccac caagtccctg tcgaagacat ggcgaagccc gcgaacgtga
       61 aactgctcga aagctggttg cgcagctatc gtccgcacga gctcttcgac cagaacggtc
      121 gccttcgtgc cgaccttcgc gaactcgcgc ccaaaggccc gcgccgcatg agcgccaatc
      181 cgcacgcgaa cggcggcatt cttcttcgcg atctgcgact gccggatttt cacgattacg
      241 ccgtgaaggt tccacaaccc ggcgccaccc tcgcgcaggc gacgcgtgtg caaggcgcgt
      301 tcatccgcga cgtcattaaa ctcaacccgg ataacttccg cgtcttcagt cccgatgaga
      361 caaactcgaa tcgctggaac gccgtgttcg aagtcacgaa ccgctgctcc accgcggaga
      421 tcatccccgg cgacgatcac gtcgcgcctg acggccgcgt gatggaggtg ttgagcgagc
      481 atcaatgcga gggctggctc gaaggctatc tgctcaccgg ccgccacggt tttttctcct
      541 gctacgaagc gttcattcac atcgtcgatt ccatgttcaa ccagcacgcg aaatggttga
      601 aagtgacgcg caatcttccc tggcgccgac caatcgcgtc gctcaactac ctgctcactt
      661 cgcacgtttg gcggcaggat cacaacggct tcacccatca ggatcccggc ttcatcgacc
      721 atgtcgtaaa caaaaaagcc gagatcatcc gcgtctatct tccgcccgat gccaacacgc
      781 tgctgtccgt caccgatcac tgcctgcgca gccgccacta tgtgaacgtc attgtcgcgg
      841 gtaaacaacc ggaagcgcaa tggctcgaca tggatgccgc cgtgaaacac tgcaccgccg
      901 gcatcgggat ctggacttgg gcgagcaacg acgaaggcgg cgagcccgac gtcgtcatgg
      961 cgtgttgcgg cgatgtgccg acgctcgaga ccctcgcggc cgtcgatttg ctgcgtcagc
     1021 atttcccacg actgaaggtt cgcgtgatca acgtcgtcga ccttatgaag ctccagtcgc
     1081 gccgcgaaca tccgcacggc ttgtccgacg ccgaattcga tgggctcttc accaccggca
     1141 aaccggtgat cttcgcgtat cacggctacc cggcgttgat tcatcgcctc acctaccgcc
     1201 gcaccaacca cgccaatatt cacgtgcgcg gctacaagga ggaaggcacc acgacgacgc
     1261 cgttcgacat ggtcgtgctg aacgagctcg accgctttca tctcgcgatg gaggtgatcg
     1321 agcgcgtgtc gcagctcggc ccgcgcgccg cgtatgaccg gcagttcttt cgcgacaagg
     1381 tgatcgaaca
//
LOCUS       DASPLT010000174         1217 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_289634, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000174 DASPLT010000000
VERSION     DASPLT010000174.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1217)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1217)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1217
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_289634"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..909)
                     /locus_tag="VEA63_01835"
     CDS             complement(<1..909)
                     /locus_tag="VEA63_01835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007358742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HYD82750.1"
                     /translation="MLATIASAALQGIDAELVHVEVNAGEAGEPKLILVGLPDAAVKE
                     SDDRVFSALSNSGYKPPRTRTTINLAPGHLRKEGPFYDLPIALGILAATLQLHAEHLG
                     EWMIAGELSLSGATRPVRGALAMARLARKLGKRGLLLPAISAEEAACVEGIPVYRVES
                     LDGAARFLSGEKPLTPLTSRSLATRAAPVDGADFSEIKGQHALRRAVEVAVAGGHNLL
                     MIGPPGAGKSMVAKRVPTIMPAPQLEEQLEIVSIHSAAGRTLSGDMAWGARPFRGPHH
                     TISDVGLLGGGTIPGPGDISLAHHGVL"
     gene            complement(940..>1217)
                     /locus_tag="VEA63_01840"
     CDS             complement(940..>1217)
                     /locus_tag="VEA63_01840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD82751.1"
                     /translation="ELEILFAEFAESDDYVHLPASRRLFKSAEDLIARTPAFWRKIVM
                     PKLENDFEGAYRYLAEPYPNGRNAYIEGVERNIAIIEARIARFSKAV"
BASE COUNT          227 a          440 c          337 g          213 t
ORIGIN      
        1 caacacgcca tggtgcgcca gcgaaatgtc gcccggaccc ggaatcgtgc cgccgcccag
       61 caagcctaca tccgagatcg tgtgatgggg cccgcgaaac ggccgcgcgc cccacgccat
      121 gtcgccgctc aacgtccgcc ccgccgccga gtgaatgctc acgatttcca actgctcctc
      181 cagttgcggc gccggcatga tcgtcggcac gcgtttcgcc accatcgatt ttcccgcgcc
      241 cggtggcccg atcatcaata aattatgtcc gcccgcgact gccacttcga ccgcgcgccg
      301 caacgcatgc tggcctttga tctccgaaaa atccgcgccg tccaccggcg cggcacgcgt
      361 cgccaacgaa cgcgaagtga gcggcgtcag cggtttttct ccgctcaaaa atcgcgccgc
      421 gccatccagc gattccacgc gataaaccgg aatcccctcc acacacgccg cctcctccgc
      481 cgagatcgcc ggcagcagca agccgcgttt gccgagcttc cgcgccaggc gcgccatcgc
      541 gagcgcgccg cgcaccggcc gggtcgcgcc cgacaaactc agttcacccg cgatcatcca
      601 ctcgccgaga tgttccgcgt gcaactgcag cgtcgccgcg agaattccca acgcgatcgg
      661 cagatcgtag aacggccctt ccttccgtaa atggcccgga gcgagattga tcgtcgtccg
      721 cgtgcgcggt ggtttgtaac cgctgttgct caacgcggaa aacacgcggt catccgattc
      781 cttcaccgcc gcatccggca gcccgaccaa aatcaacttc ggctcgcccg cctcaccggc
      841 gttgacctcc acgtgcacga gctcggcgtc gatcccttgc agcgcagcgg aagcgatcgt
      901 ggccagcatg tttgttgcaa tgacggtgac agccgtccgc tagacggcct tcgagaatcg
      961 cgcgattcgg gcctcgatga tcgcgatgtt gcgctccacg ccctcgatgt aggcgttgcg
     1021 accgtttgga taaggctccg cgagataacg atacgcgccc tcgaaatcgt tctccaattt
     1081 cggcatcacg atcttccgcc aaaaagccgg cgtgcgcgcg atcagatcct cggccgattt
     1141 gaacaggcgt cgcgacgccg gcaggtgaac gtagtcgtcc gactccgcga attcggcgaa
     1201 aagaatttcg agttcct
//
LOCUS       DASPLT010000175         2235 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_24014, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000175 DASPLT010000000
VERSION     DASPLT010000175.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 2235)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2235)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2235
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_24014"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1124
                     /locus_tag="VEA63_01845"
     CDS             <1..1124
                     /locus_tag="VEA63_01845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD82752.1"
                     /translation="LLKSIFTWGAESGAKASFAAKRRAWRGAVVACVVFGGAVQAMAV
                     REVSGPTFAQATPETPSSPERPPLDELVYADGDRVRGHFVERSGDVLVFRSERFGLLR
                     VPAAEAEVILVKSAEPAVAEAAEHDDKVEIAPEWEWWPLSPLAMTRALKDFFGSWHGR
                     FSAGAEVLQDVSDHNSATVEARLNRKWERDEVQINGRYDYSSVNEKAATDMVRADGLF
                     RHDFPGKLFAVYRPSLEWNRAYYRSGVPSDYVLLQQELGAGVNILNTPARKVRVGVSE
                     NAFDTWVLPTETHISQTVESLFAEVEAKLPWRVTLTNRGVWYYSFADSTDGWENRFEI
                     SKKLTETLTIGARHETRRNNPDVRSADYERLRLLFGFDF"
     gene            complement(1293..>2235)
                     /locus_tag="VEA63_01850"
     CDS             complement(1293..>2235)
                     /locus_tag="VEA63_01850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="WD40 repeat domain-containing protein"
                     /protein_id="HYD82753.1"
                     /translation="LPGHTDGTNCLAWQPPIKTQNAKPETLSSLATGGQDGAVKLWDA
                     LAGQHTATAELGTAWIEHLAWRPTPSSAPDSGSVSGSQRSALSAQLLFASAGKNLFAL
                     RADATVAHTFKPAPKSISALAWQPAGGCAAVSFFGGVCLWDADDFVAQKEYPYANGIA
                     ALVWSPDNKWLVSGNHDPSVHLWIPESDTEFHMSGYEGKVKHLSFDHTSRWLATGGGR
                     DACVWDCSGHGPEGREPGMFPHDAPVCALAFQNAHGLLATASADGVVQLWSPERKQPL
                     RATVRMPAAAAKLVWSPDDRQLAIGTDKGHVFVLKSE"
BASE COUNT          360 a          571 c          871 g          433 t
ORIGIN      
        1 ctttgttgaa atcaattttt acctggggcg cggaatccgg cgcgaaagcg tcgttcgcgg
       61 cgaagcgtcg ggcgtggcgt ggcgcggtgg tggcgtgtgt cgtattcggt ggcgcggtgc
      121 aggcgatggc ggtgcgagag gtctcgggcc caacgtttgc gcaggcgacg cctgaaacgc
      181 cgtcgtcgcc ggagcgaccg ccgttggacg agttggttta tgcggatggc gaccgggtgc
      241 gcgggcattt cgtcgagcga tcgggcgacg tgctggtgtt ccggtccgag cgatttggat
      301 tgttgcgcgt gccggccgcg gaggcggaag tgattttggt gaagtcggcg gagcccgccg
      361 tcgcggaggc agcggagcac gacgacaagg tggagatcgc gccggagtgg gagtggtggc
      421 cgctgtcgcc gctcgcaatg acgcgggcgt tgaaagactt tttcggttcg tggcacggcc
      481 ggttttcggc gggcgccgag gtgcttcagg acgtgagcga ccacaacagc gcgacggtcg
      541 aggcgcggtt gaatcgcaag tgggagcggg acgaagtgca gatcaacgga cgctacgatt
      601 acagctcggt gaacgagaaa gcggcgacgg acatggtgcg ggcggacggc ttgttccggc
      661 atgattttcc ggggaagctg ttcgcggttt accgtccgtc gctcgagtgg aatcgcgcct
      721 attatcggtc cggcgttccg tcggattacg tgctgttgca gcaggaactc ggtgcgggtg
      781 ttaacatttt gaatacgccg gcgcgcaagg tgcgcgtggg cgtgtcggag aatgcgttcg
      841 acacgtgggt gctgccgacg gagacgcata tttcgcaaac ggtggagtcc ctgtttgccg
      901 aggtcgaagc caagctgccg tggcgggtga cgctgacgaa tcgcggcgtg tggtattact
      961 ctttcgccga cagcaccgat ggctgggaga atcgtttcga gatcagcaaa aagctgacgg
     1021 agaccctgac gattggcgcg cggcatgaga cgcggcggaa caatccggat gtgcgctcgg
     1081 cggattacga gcggctgcgg ctgttgttcg gtttcgattt ctgaggtggc ggtgttccgg
     1141 agcatttcca agaaaactgt agccgcttct ggagggacga cctcctgtcg tccgccgcgg
     1201 cactgaggcc ggcccttcgt ctgaccggct atggttttct gaaaacgctg tagcggcggg
     1261 tcaggagacc tcgccctaca tgggacgcgg gcctactcgg atttgaggac gaagacgtgg
     1321 cctttatcgg tgccgatcgc gagttggcgg tcgtcggggg accagacgag tttcgccgcg
     1381 gcggcgggca tgcggacggt ggcgcggagc ggctgcttgc gctcggggct ccagagttgc
     1441 acgacgccgt cggcgctcgc ggtggcgaga agaccgtgcg cgttttgaaa tgccagggcg
     1501 cagaccggcg cgtcgtgggg aaacataccg ggttcgcggc cttcggggcc gtggccgctg
     1561 caatcccaga cgcaggcgtc gcgaccgccg ccggtggcga gccagcgcga ggtgtgatcg
     1621 aaggagaggt gtttcacttt tccctcgtag ccgctcatgt ggaattcggt gtcgctttcc
     1681 ggaatccaga gatgaacgga cggatcgtgg ttgccggaga cgagccattt gttgtcgggc
     1741 gaccagacga gggcggcgat gccgttggcg tagggatatt ctttttgcgc gacgaaatcg
     1801 tcggcgtccc agaggcatac gccgccgaaa aacgaaacgg ccgcgcagcc gccggcgggt
     1861 tgccaggcga gcgcggagat ggatttgggc gcgggtttga aggtgtgcgc gacggtggcg
     1921 tcggcgcgga gggcgaagag atttttgccg gctgaggcga ataagagctg agcgctgaga
     1981 gctgagcgct gagagcccga gacggaaccg gaatcgggtg ctgaagacgg cgtcgggcgc
     2041 caggcgaggt gttcgatcca ggcggtgccg agttcggcgg tggcggtgtg ttggcccgcg
     2101 agggcgtccc agagtttgac agcgccgtcc tggccgcccg tcgcgagcga cgagagggtt
     2161 tcgggttttg cgttttgggt tttgattggg ggctgccacg cgaggcagtt ggtgccatcg
     2221 gtgtggccgg ggagt
//
LOCUS       DASPLT010000176         7787 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_268351, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000176 DASPLT010000000
VERSION     DASPLT010000176.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 7787)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7787)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7787
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_268351"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1207)
                     /locus_tag="VEA63_01855"
     CDS             complement(<1..1207)
                     /locus_tag="VEA63_01855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD82754.1"
                     /translation="MSEFDKADTLHRLVKQLIDSGAAKTVAEAEAIFAGFRVALVIAD
                     EAAKTSDGQIQLLTAIVLARRVFLGGVTVSGPLDQPLLAPLPYGDTLREAVCELGGKA
                     GDAIDDLPTILIAGSVQPRRAGFCVRTLAAGWRGGIVAADADVEIEGATPMPLASMLA
                     AALAINETFLHVSAAHSVAGRRQVGLSLWDPADVDWLIAPPVEPALAYLPSDLWLIGL
                     GHLGQAYLWGLGLLPYAPVTKLKLVLQDIDIITPSTESTSVLSDAGLIGAKKTRAMAA
                     WAEQRGFETAICERRFDAGFTRRDDEPGVALCGLDNALGRRALDSVGFDLIVEAGLGR
                     GHRNFRTMRLHVLPGTRPAIEIWREREAPAAPATAPAYQDLLKRGELDKCGMALLAGK
                     AVGAPFVGAV"
     gene            complement(1223..1603)
                     /locus_tag="VEA63_01860"
     CDS             complement(1223..1603)
                     /locus_tag="VEA63_01860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD82755.1"
                     /translation="MLGVRDEMGRARIEDFVLYDDLDPNALSRGIVHFNGRYFGDLWA
                     ICEARNLAVVADVHVHPGGSGQSDSDRTYPMISRKGHLALILPDFADPPVKRQRIGIY
                     RYEGAKLWHTIPPRMREAFFHIGF"
     gene            complement(1692..2162)
                     /locus_tag="VEA63_01865"
     CDS             complement(1692..2162)
                     /locus_tag="VEA63_01865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD82756.1"
                     /translation="MAPDERALRGDIAKAAYLLAEAEGRWRITSIAWPYVIASVIAKD
                     GIAHILRLNCAGYPQAPPTGGPWDIERNAILAFDRWPRGSGGRVSAVFRPDWKNGSAL
                     YLPCDRESITGHDNWRHETPSKIWRPAAGLAQYLELVHELLHCRDYVPVARAAA"
     gene            complement(2162..2671)
                     /locus_tag="VEA63_01870"
     CDS             complement(2162..2671)
                     /locus_tag="VEA63_01870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD82757.1"
                     /translation="MKTIDIFYQGEGLAEILHLEIEPDASFADVKARLSEKHGFDAEA
                     IIFLEDDEEPIAETARVSERAKKSGVKIHLHRCRQIDVAVTFNRETVNHRFAPGATVA
                     RVKHWAAVKKFGMSEEEAGEHVLQIAGTHDRPNAGTHIGTLTDRKTCALALDLVPDER
                     INGALEALR"
     gene            complement(2763..4208)
                     /locus_tag="VEA63_01875"
     CDS             complement(2763..4208)
                     /locus_tag="VEA63_01875"
                     /inference="COORDINATES: protein motif:HMM:NF024117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HYD82758.1"
                     /translation="MDGLSLAGKTGLCRLADDMAGIERSSAWELLSAYLLDRTDLART
                     LASATTITGQMRAVAVWQFLNFVRDHSPVGSGYPLRRTLDRVRQLVLLAEERDLRQVP
                     AAALHLDAVRLMTVHASKGLEFDAVHAPGLTVSSFPSSYRGQRCPPPEGMIETEEAAD
                     PKRAHEDEEECLFFVALSRAKKRLSLTLARRQPNGNNRKPSPYLDWVASNVNEIAAPD
                     MLALPAHAIRPAPIVLQLGGAWTITDARLISYEKCPRRFFYTHVLNLGGGRKPTAFTR
                     THDCLYDLISWLAEERSSAAPSVADAEAAFEKIWQERGPTDHAFAGDYRRLALRMVAA
                     LVEAGAGRRFLNAELLAIDFANGRVVVAPDEVAELADGTIVVRRVRTGYKRTDEYDRL
                     DYALYQLAAEARFGPGAIVEALHLTDETAEPVPRSANKLNTRKAKSEAMLGQIAAGWF
                     PPAPDAVTCPRCPHFFICAALPAGPLSLEES"
     gene            complement(4156..6159)
                     /locus_tag="VEA63_01880"
     CDS             complement(4156..6159)
                     /locus_tag="VEA63_01880"
                     /inference="COORDINATES: protein motif:HMM:NF012789.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HYD82759.1"
                     /translation="MDPFELVRREAEKLHDLVVEKGGEELSPLSLTQAAARELGVELA
                     WLEKGDPALKNARALFDVQSGLIICQSGGSDAERALLVAHEIGHVVTHAGSTTCATND
                     IDPSRSTESAPVGLQRVEEYGAHERRELQANVFAREFLLPRTLARRLHMVDAKTSVDI
                     AVVTGLPKSLVRQQLFDALLLPPAPAEAEPVESKPLKPDPSQDRAVAHRGSPFQLQAG
                     PGTGKTRTLVKRVQSLLVEGVDPASILVLTFSNRAAGELAERVTRAAPDAAPKIWIGT
                     FHAFGLDLIRRHHDLFSLSANPPLFDRSDAIEVLEEILPTLPLVHYRNIWDPALVLRD
                     VVQAISRAKDELVDAQRYRALAHAMAERAADADAMKAAGKALEVAHIYELYERALRVY
                     GGVDFGDLLMRPALMLETDEALRTTVRLRHRHVLVDEYQDVNRASARLLKAIAGDGQR
                     LWVVGDARQSIYRFRGASSVNMAAFTADYPSAVIDKLTVNYRSTQNIVDTFQAVSRHM
                     RAAREMLPLELIAAEGSSSAADPESRRFDSLEDEGAGVAACVRALEADGVKLRDQAVL
                     CRSNDRLNAIAAALEQRDIPVLHLGGSTRPPVHLESCGRSLWRRACSHRRHASLRGVP
                     AGYVCRAPACSRCAQIAAQGASLSRRGGRLVTRGQDRALPASR"
     gene            complement(6159..6818)
                     /locus_tag="VEA63_01885"
     CDS             complement(6159..6818)
                     /locus_tag="VEA63_01885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD82760.1"
                     /translation="MNLTSSAPTLREALYELAMTDRALDAALLDEFVRRFPQYSQELT
                     DFAVEQALEALSERADSDGVAQHATVTPEVSRALSAFHNQLHVIAQSANHRSEQNRLP
                     DDAGIAINPFEALSRDDFRGLARRMKVNTVFLGKLRDRQLDPSTIPERFLEMVAREAR
                     APLSVVVAHFAAAPVEIRSGKQFYKADEKPQAIKRQSFDEAVRGSGLSQEEQADLLRL
                     L"
     gene            complement(6821..7579)
                     /locus_tag="VEA63_01890"
     CDS             complement(6821..7579)
                     /locus_tag="VEA63_01890"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HYD82761.1"
                     /translation="MARPLTRRKEDGALYARPPNIEREIDLALSQDEDTLKRRLSVTM
                     RTAADYLSSECLVHLIRRSLSRNDRSLADLCMPVLLMRCHANLLAQVPNGRLPNAAAL
                     REDILQEFGVMVATDSADEALNELDYFECRFNSAFRAFRIDRVRTETRHQNRFVPAPE
                     KIDDVDLEEYEGTLRKVAEAFVPAAQEDAAFLKVVRAAIDGLPEDERNALILCYALGF
                     SEKSAATRCNCTDRTIRNRLSRAAQKLKALKEDM"
BASE COUNT         1550 a         2424 c         2350 g         1463 t
ORIGIN      
        1 ccacagcgcc cacgaacggt gcgccgaccg ctttgccggc tagcaacgcc atgccgcact
       61 tgtcgagttc tccgcgcttc aagagatcct ggtaggcagg cgctgttgcc ggagcggctg
      121 gcgcttctcg ctcacgccaa atctcaattg cagggcgtgt ccccggcagc acgtggaggc
      181 gcatggtgcg aaagttgcgg tgcccgcgcc caaggcctgc ttccacgata aggtcgaagc
      241 caacgctatc gagcgcgcga cggcccaacg cattgtcgag accacacagc gccaccccgg
      301 gttcatcgtc gcgacgggtg aaaccggcgt cgaaccggcg ttcacagata gccgtttcga
      361 agccccgctg ttcggcccat gccgccatgg cgcgcgtctt cttcgcgcca atcaggcctg
      421 catccgacaa caccgacgtg ctctcggtcg agggcgtgat gatatcgatg tcttgcagca
      481 cgagtttgag cttggtcacc ggcgcgtagg gcaagaggcc aagaccccag agatacgctt
      541 ggcccaaatg accaagacca atcagccaca ggtccgacgg caaataagcg agcgcgggtt
      601 caaccggcgg cgcgatcagc cagtcgacgt cggcgggatc ccaaagcgag agtccgactt
      661 ggcgacgtcc ggcgacgcta tgtgctgcac tcacatgcag gaaggtctcg ttgatggcga
      721 gtgccgcggc gagcattgaa gccagcggca tcggcgtcgc gccttcaatc tccacgtcag
      781 catcggcggc aacaatgcct ccgcgccaac cggccgccag cgtgcgcaca caaaagccgg
      841 cgcgccgcgg ctgcacagag ccggcgatca ggatcgtcgg cagatcgtcg attgcatcgc
      901 cggctttacc gcccagctcg cacacggctt cgcgcaaggt atcgccatag ggaagcggcg
      961 ccagcagcgg ctgatccagc gggccggaca ccgtgacgcc gccaaggaac acgcgacgcg
     1021 ccagaacaat agcggtcagc aattggattt gcccatcgct ggtcttcgcc gcctcatcgg
     1081 caatcaccag cgcgacgcga aagccggcga agatcgcttc cgcctcggca acggttttgg
     1141 ctgcaccgct gtcgataagc tgcttcacca ggcggtgcag tgtgtcggcc ttgtcgaatt
     1201 cgctcatgcg cgtcttcgcc tcctagaaac cgatatgaaa aaacgcctcg cgcatgcgcg
     1261 gcgggatggt gtgccaaagc ttggcgcctt cgtagcgata gatgccgatg cgctggcgct
     1321 tcaccggggg atcggcaaaa tccggcaaaa tcagcgccaa gtgccccttg cgcgagatca
     1381 tgggataagt gcgatcggaa tcgctttgcc cggaaccgcc cggatggacg tgcacatcgg
     1441 cgacgacggc aagattgcgc gcttcgcaaa tcgcccagag gtcgccgaag taacgcccgt
     1501 tgaagtgaac gatgccgcgc gagagcgcgt tcgggtcgag atcgtcgtaa aggacgaagt
     1561 cctcgattcg agcacggccc atttcatcgc ggacgcccag caagaatgcg ccgctttcgc
     1621 gtgtgcgccc acgtccgcgg gcgcgcaacg caatcaacag acgccgccac aggagccacg
     1681 agcaagacaa ttcatgccgc ggcgcgagca actggcacat agtcgcggca gtgaagaagt
     1741 tcatgtacaa gctccaggta ttgggcgagc ccggcggcgg ggcgccagat tttcgagggc
     1801 gtctcgtggc gccaattgtc gtggccggtg atgctctcgc ggtcgcacgg caagtagagc
     1861 gcgctgccgt tcttccagtc cgggcgaaac accgccgaca cgcgcccgcc tgatccgcgt
     1921 ggccaacgat cgaaggcaag gatggcgttg cgctcgatgt cccacggacc accggtcggc
     1981 ggggcttgcg ggtaaccggc gcaattgagg cgcaaaatgt gcgcgatgcc gtccttggcg
     2041 atgaccgacg caatcacgta gggccaagcg atactggtga tgcgccatcg cccttcggcc
     2101 tcagccaaca ggtaggccgc tttcgcgatg tcgccgcgca gcgcccgctc gtcgggcgcc
     2161 attagcggag agcctcgagc gcgccgttaa tgcgctcatc gggaacgagg tcgagagcaa
     2221 gcgcacaggt tttgcggtcg gtcaacgtgc cgatatgcgt cccggcattg gggcgatcat
     2281 gcgtgccggc aatttgcaac acgtgttcgc ccgcctcttc ctcgctcatg ccgaacttct
     2341 tcaccgccgc ccaatgtttg acacgcgcga ccgtggcgcc cggcgcgaag cggtggttga
     2401 cggtttcgcg gttgaacgtg accgcgacat ctatttgacg gcaacggtgc aggtggattt
     2461 tcacgcccga cttcttggcg cgctcgctaa cacgcgcggt ctcggcgatc ggctcttcgt
     2521 cgtcttcgag aaaaatgatc gcctcggcgt caaagccgtg cttttcgctg agacgcgctt
     2581 tgacgtcagc gaagctcgcg tccggctcga tttcaagatg cagaatttcg gcgagccctt
     2641 cgccttggta aaagatatca atggtcttca tgaacgatac tcccaatgga tcggcgacac
     2701 tcgtcgcctt tggtcctcac tgggttgatc gtttcgcccg gcgaaagccg gaaatccccg
     2761 cctcaacttt cttcgagtga gagcggtccg gccgggagcg ccgcgcaaat gaagaagtgg
     2821 gggcaacgcg gacaggtgac ggcgtcgggg gctggtggaa accaaccggc ggcgatttgg
     2881 cccaacatcg cttcactctt agccttgcgc gtgttgagct tgttcgcgct cctcggaacg
     2941 ggctccgcag tttcgtcagt cagatgcagc gcctcaacaa tggcgccagg gccgaaccgc
     3001 gcctcggcgg ccaactgata aagcgcatag tcgaggcgat cgtactcatc ggtacgtttg
     3061 tagcccgtgc gcacgcggcg cacgacgata gtgccgtctg ccagctccgc gacctcatcg
     3121 ggcgccacaa cgacccgtcc atttgcgaaa tctatcgcca gaagctcggc gttgagaaag
     3181 cggcgtccag cgccagcttc aaccaatgcc gcgaccatcc gtaaagctag ccgccgataa
     3241 tcgccggcga aggcatgatc ggttggacca cgctcttgcc agatcttctc gaacgcggcc
     3301 tcagcatcag ccaccgatgg ggccgccgaa ctacgctcct cggcgagcca actaataagg
     3361 tcgtagagac aatcgtgggt gcgcgtgaac gcggtgggct tgcgcccgcc ccccaggttc
     3421 aggacgtgag tatagaagaa acggcgtgga catttctcat aggaaatcag ccgtgcgtcg
     3481 gtgatggtcc aggcgccgcc cagttgcagc acgatcgggg ccgggcgaat cgcgtgcgcg
     3541 ggaagtgcca acatgtcggg cgcggcgatt tcattaacgt tggacgccac ccagtccaaa
     3601 tacggcgaag gcttgcggtt gtttccgttc ggctggcgac gcgccagcgt cagactcaac
     3661 cgtttcttag cccgcgatag agccacgaag aacagacact cctcctcgtc ctcgtgcgct
     3721 cgttttggat cggcggcttc ttccgtttcg atcatgccct caggcggggg gcagcgttgc
     3781 ccacgatagg aagaaggaaa gctcgacacc gtgagccccg gtgcatggac cgcatcgaat
     3841 tccaggccct tgctagcatg cacggtcata agacgcacag catccaagtg aagcgccgcg
     3901 gctgggacct ggcgcaggtc ccgctcttcg gccagcagca caagttggcg aactcggtcg
     3961 agcgtgcgtc gaagcggata gccgctgccg accggactat ggtcgcgcac gaaattcaga
     4021 aactgccaga cagcgactgc acgcatctga ccggtgatgg tcgtagcgct cgcgagcgtg
     4081 cgcgctaaat cagtgcgatc aagcaaatag gcggacaaca attcccaggc cgagctgcgt
     4141 tcgatcccgg ccatatcatc tgctagccgg caaagcccgg tcttgcccgc gagtgacaag
     4201 ccgtccacct cggcgagaga gcgaagctcc ttgagcggcg atttgggcgc atctcgaaca
     4261 tgccggagcg cgacatacat atcctgcagg gacaccgcgt aacgaggcat ggcgcctatg
     4321 cgaacaagcg cgtcgccaaa gggatcgacc gcaagactca agatggacag gaggtcgcgt
     4381 acttcctccg aggtgcaaca cgggaatgtc gcgttgctct agcgccgcag cgatagcatt
     4441 cagccggtca ttggaacgac aaagcaccgc ctggtcgcgc aacttgaccc cgtccgcctc
     4501 tagagcacgc acacaagcgg ccacccctgc gccctcatcc tctaaactat cgaaccgcct
     4561 cgattccgga tcagctgccg atgatgatcc ttcggccgca atgagttcga gcggcaacat
     4621 ttcccgcgcg gcgcgcatgt gtcgggacac cgcctgaaag gtgtcgacga tattctgggt
     4681 tgatcgataa ttgacagtga gtttgtcaat cactgcactt ggatagtccg ccgtaaacgc
     4741 cgccatattg acggatgaag cgccgcggaa gcgatagatg gattggcgcg cgtcgccgac
     4801 gacccacaag cgttggccat caccggcgat agctttcagt aaacgcgcgc tcgcacggtt
     4861 cacgtcctgg tattcgtcga ccaggacatg acggtgacgc agccgcaccg tcgtacgcag
     4921 cgcctcgtcc gtttcgagca tgagcgcagg gcgcatgaga aggtcgccga aatcgacgcc
     4981 gccatacacg cgtagcgcac gttcgtacaa ttcgtagatg tgcgcgactt caagcgcttt
     5041 ccccgccgcc ttcatcgcgt cggcgtcggc tgcgcgctcc gccatcgcat gcgccaaggc
     5101 acggtagcgc tgtgcgtcca ccaactcgtc cttagcccgc gatatcgcct gcacgacatc
     5161 gcgcaacacc agcgccgggt cccatatgtt gcgatagtgg acgagcggca gtgtcggcag
     5221 gatctcttcg agcacttcga tggcgtcgct gcggtcaaac aatggtgggt tagccgacag
     5281 cgaaaagaga tcgtgatgcc ggcggataag gtcgaggccg aaagcgtgaa aagtgccgat
     5341 ccagatctta ggtgcggcat cgggcgcagc gcgggtcacg cgttcggcga gttcgccggc
     5401 ggcgcggttc gaaaatgtga gcacaagaat cgacgcggga tcgactcctt cgactaacaa
     5461 cgactgcacg cgtttgacca aggtgcgggt ttttcctgtt ccaggccccg cttgcagctg
     5521 aaacggactg cctcggtgcg caacggcccg atcctgtgac ggatcgggct taagcggctt
     5581 ggactcaacg ggctccgcct ctgccggtgc cgggggcaag agcaacgcgt cgaacaattg
     5641 ttgacggacg aggctcttcg gcaaacccgt cacgactgca atgtcgaccg aggtcttcgc
     5701 gtcaaccata tgcagacggc gcgctaatgt gcgcggcaac agaaactcgc gcgcaaagac
     5761 attggcttgg agttcgcgcc gttcatgggc gccgtattcc tcgacccgtt gcaagccgac
     5821 cggcgcagac tcagttgaac ggcttgggtc tatgtcgttc gtcgcacacg tcgtcgagcc
     5881 cgcgtgggtg acaacgtgac cgatctcgtg tgcgacaagc agtgcgcgct cggcatctga
     5941 accgccgctt tggcagatga tgagcccgct ttgcacgtca aaaagcgcgc gcgcattctt
     6001 gagggcgggg tcgccctttt ccaaccatgc cagttcaaca ccaagctcac gcgccgcagc
     6061 ttgggtgaga gaaagcggcg acaactcctc accgcccttc tcaacaacca gatcgtgaag
     6121 tttctcggcc tcccgtctga cgagctcaaa cgggtccatt acaagagacg gagcaggtcc
     6181 gcctgctctt cttgacttag cccggaacct cggacggcct cgtcgaagct ctgacgtttg
     6241 atcgcctgcg gtttttcgtc agccttatag aattgcttgc ctgatcgaat ttccaccgga
     6301 gccgccgcga agtgagcgac gacgacactt aagggggctc tggcctcacg cgcgaccatc
     6361 tctaagaatc gctctgggat tgtacttggg tctagctggc gatcacgcag ctttcccaga
     6421 aacaccgtgt tcaccttcat gcgccgcgcg agtccgcgaa aatcatcacg cgacaaggct
     6481 tcaaagggat tgatcgcgat ccccgcatca tccgggagcc tgttctgctc tgatctatga
     6541 tttgcgcttt gcgcaatgac gtggagctga ttgtgaaacg cgcttagggc gcgcgatacc
     6601 tctggtgtca ccgtagcatg ctgcgcgacg ccatccgaat ctgcccgttc cgacaatgcc
     6661 tcaagcgctt gctcaactgc aaaatcggtg agttcctggc tgtattgcgg aaaccgccgc
     6721 acgaattcgt ccaacagcgc ggcatcgagt gcacggtcgg tcattgccaa ttcatagaga
     6781 gcctcgcgca gcgtcggcgc gctggaggtc aagttcatgt tcacatatcc tccttcaaag
     6841 ccttgagctt ttgggccgca cgcgacagtc gattacggat cgtgcggtca gtgcagttgc
     6901 aacgtgtagc tgccgatttt tcgctgaagc cgagcgcata gcaaagaatg agcgcgttgc
     6961 gttcgtcttc aggtaatccg tcgatcgccg cgcgtacaac ttttaggaac gctgcatctt
     7021 cttgcgcggc agggacaaat gcctcggcaa cctttcgaag cgtaccctcg tactcttcca
     7081 ggtcgacatc atcgattttt tcgggcgcgg gcacgaagcg gttctgatga cgcgtttcgg
     7141 tccgcacgcg atcaattcgg aacgcgcgga aggcgctatt gaagcggcac tcaaaatagt
     7201 caagttcgtt gagcgcttcg tcggcgctgt cagtcgcaac cattacgccg aattcctgga
     7261 gaatatcttc ccgcagcgct gctgcgtttg gcaacctgcc gttcgggacc tgtgcgagca
     7321 ggttcgcgtg acagcgcatc aacaaaacgg gcatgcagag atctgcaagg ctacggtcat
     7381 tgcggctcag ggagcgccgg atgagatgca ctaggcactc agaactcaaa tagtcagccg
     7441 cagtgcgcat ggtgacggaa agcctgcgct tcagcgtgtc ttcgtcctgg ctcaaggcca
     7501 agtcgatttc ccgctcaatg ttcggcggac gcgcatagag cgctccgtct tccttgcgtc
     7561 tggtcagggg tcgggccatc gtgctgcctt gctcctatcg tcggcggcca gacgcatccc
     7621 tcgcctctgc ccatcctggc cttgatcgat ttccaatcgc aaacccggaa agcgcgcctc
     7681 gcaaacggcc ctgctatcct cagttgtagc cgattcttcg ccaaaaacgt gggtttgggg
     7741 tgtagccgag ccgatccccg ggtcagggcc gccgccaaga gcagttc
//
LOCUS       DASPLT010000177         3585 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_63392, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000177 DASPLT010000000
VERSION     DASPLT010000177.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 3585)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3585)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3585
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_63392"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..736)
                     /locus_tag="VEA63_01895"
     CDS             complement(<1..736)
                     /locus_tag="VEA63_01895"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009510527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmE family RNA methyltransferase"
                     /protein_id="HYD82762.1"
                     /translation="MPDFRSYCSPGSAEPSEIVLSADESHHLVVVNRARLGDTVVAFD
                     GRGTEWICELTSDRKAGATLKVRFRQKAKPLAYEITLGQALPKGTSMDAIVRKATEIG
                     VARIVPLESERTQVHLDADRSDKKIDKWQTAALEAAKQCGNPFLPEIAPVQKAAAFME
                     SARGYDLKLIASLQPGAKSLKHVLANFLSAQGRAPTKVLWLVGPEGDFTPAEMSLSAN
                     AGFEPITLGPLVLRCETAATYALSVLS"
     gene            complement(1265..1666)
                     /locus_tag="VEA63_01900"
     CDS             complement(1265..1666)
                     /locus_tag="VEA63_01900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009508319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD82763.1"
                     /translation="MNINDPTSGRILVHGRGTGGIGRDDILRRARELAAIDGRSGADP
                     TDDDIARARIELLGTDLPATTVEDDEARSGLTRDPSEPPSIPGREKPMHEGPDEEKAV
                     ERMALEGVEEAQHDQMLAARRRERRLDEEET"
     gene            1903..2760
                     /locus_tag="VEA63_01905"
     CDS             1903..2760
                     /locus_tag="VEA63_01905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012372874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045027 - DNA end binding [Evidence IEA];
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ku protein"
                     /protein_id="HYD82764.1"
                     /translation="MRALWKGSISFGLVNIPVSLYPATRREELSFRLLRSTDLSPVNY
                     KRVAQADGREVPWDQIVKGYEYEKGKFVVLKEDDFKRVDIEATQTVDIMDFVQLSEVD
                     PMFFQKPYYLVPEKGGVPAYQLLHDVLTDTGKAGVAKVVIRTRQHLAALKAQKNALVL
                     ELMHFGDELVDVEELNIPSSKSGARKRELDMAKALVEQMTDKWDPSRYTDDYTSAVMA
                     LIKDKIEHGGRTTTAAPKARKQTNVIDLAAVLQQSLNEAGGGGARKKSGGKAAKGRGK
                     GKTKTRKAA"
     gene            3046..>3585
                     /locus_tag="VEA63_01910"
     CDS             3046..>3585
                     /locus_tag="VEA63_01910"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HYD82765.1"
                     /translation="MPTVAPAHSINVLVVEDHEDDFRYLSFLLGRNSIGSYKLQWAPS
                     YSAGQKAIRAGGFDVALFDYRLGGGTGIDLLKEAQAAGCEKPIILLTGHDSQEVDQEA
                     SQAGAADYLCKTGLNGTELERAIRYAMQQAAMTAALRQSQQQLELFMRSVPCAVCIRD
                     DAGRLLFQNDLFARYFKNED"
BASE COUNT          724 a          939 c         1227 g          695 t
ORIGIN      
        1 agctgaggac gctgagggcg taggtggcgg cggtttcgca gcggaggacg agtggcccaa
       61 gcgtaatcgg ttcgaagccg gcgtttgctg agaggctcat ttccgcgggc gtaaaatcgc
      121 cttcggggcc gaccagccaa aggactttcg tcggcgcgcg accttgggcg gaaaggaaat
      181 ttgccagcac gtgtttcaag gatttcgcgc cgggctgaag gctggcgatg agtttcaggt
      241 cgtagccgcg cgcggattcc atgaacgcgg cggctttttg gacgggtgcg atttcgggaa
      301 gaaagggatt gccgcattgt ttggcggcct cgagcgcagc cgtctgccac ttgtcgattt
      361 tcttgtcgga gcgatcggcg tcgaggtgca cctgggtgcg ttcgctttcg agcggaacga
      421 tgcgggcgac gccgatctcg gtggccttgc gcacgatggc gtccatcgag gtgcctttcg
      481 gcagcgcctg gccgagggtg atttcgtagg cgagtggttt ggctttttgg cgaaagcgaa
      541 ccttgagcgt tgcgccggcc tttcgatcgc tggtgagttc gcaaatccac tcggtgcctc
      601 ggccatcgaa ggcgacgacc gtgtcgccaa ggcgcgcgcg gttgacgacg acgaggtgat
      661 gcgactcgtc agctgagaga acgatttcgg aaggttccgc gctgccgggg ctgcaatagc
      721 tgcgaaaatc aggcatgcgg cagtgaagag ttcccctgag cgcctgacaa gtgtgcgaga
      781 accggcgcgg tcgaacgcgc gatgctggtg atggagcccc ttaaaacagt aagggcgctg
      841 gtctaagacc agcgcccctg ccgactttaa ctacaaacta tgaactacac taaaaagaac
      901 gtaacaccta acacacgtcg agaaagacgc ggcggtctgc agaacgttca aaaaagaaaa
      961 caaatcggga aatttttttc ggaagacagc ctttgaggaa agcagccgcg gctcaggtcg
     1021 atgatcgcca gtgagttttg agagcgcaga aaacgcgggc gaaagagaaa aaattttcga
     1081 tcacaggtgc gagcgagtga gagatggcgg cgagtgaggc ggacaaacgc agtgcaaaca
     1141 gtcatcctca cgaagtcgca catcgagagt aagcccgaga ggtgaactcg aggtcgtcgg
     1201 gctgtttgcg agcgaaacgt cagcaagccc gacgaggacg tcgggctcca ccttctgagt
     1261 tcgactaggt ttcttcttcg tccagccgac gttcgcggcg gcgggctgcg agcatttggt
     1321 cgtgctgggc ttcttcgacg ccctcgaggg ccatgcgttc aacggccttt tcttcgtcag
     1381 ggccttcgtg catcggcttt tcacggccgg ggatcgaggg cggttcgctc ggatcgcgcg
     1441 tgaggccgga gcgtgcttcg tcgtcttcga ccgtggtggc gggcaaatcc gtccccaaga
     1501 gttcgatacg cgcgcgcgcg atgtcgtcgt cggtgggatc ggccccggag cggccgtcga
     1561 tggcggcgag ctcgcgtgcg cggcgaagga tatcgtcgcg gccgatgccg ccggtgccgc
     1621 ggccgtggac cagaattctg ccggaggtgg gatcgttgat gttcatgagt gaaatcgttg
     1681 agggatgggt gaaggcaaag attgaaatga gctgattcag cgcggctgcc gcgcgaagcc
     1741 tttgttttgg caatctactc catgtgcgag ttgtcaccat ccagcgcggt gctgtggtgg
     1801 atgtcgggcg gtggcccggt gacctccgcg tcggataaac caccacgact ccgtcggggc
     1861 gggttgcggt ggcgcgggcg cgaaacgtgg ctaagatgcc cgatgcgtgc actctggaaa
     1921 ggctcgatca gtttcggctt ggtcaacatt cccgtgtcgc tttatccggc gacccgacgc
     1981 gaggagctca gttttcggct cctgcgctcc accgatctca gcccggtgaa ctacaagcgt
     2041 gtcgcgcagg ccgacggccg cgaggtgccg tgggaccaga tcgtgaaggg ctatgaatac
     2101 gagaagggaa agttcgtcgt gttgaaggag gacgatttta aacgcgtcga catcgaggcg
     2161 acgcagacgg tcgacatcat ggattttgtg cagctatccg aggtcgatcc gatgtttttt
     2221 caaaagccgt attatctggt cccggaaaag ggcggtgtgc cggcttatca gttgttgcac
     2281 gacgtgctga cggacaccgg caaggcgggt gtcgccaaag ttgtgatacg cacgcgccag
     2341 cacctcgcgg cgttaaaggc gcaaaagaac gcgctggtgc tcgagctgat gcatttcggc
     2401 gacgaactgg tggacgtcga ggagttgaac atcccctcgt caaagagcgg cgcgcgcaag
     2461 cgggagctgg acatggcgaa ggcgctcgtc gagcagatga cggacaagtg ggatccgtca
     2521 cgttacacgg acgattacac atcggcggtg atggcgctga tcaaggacaa gatcgaacat
     2581 ggtgggcgca cgacgacggc tgcgccgaaa gcgcggaaac agacgaacgt gatcgatctc
     2641 gcggcggtgc tgcagcagag cttgaacgag gcgggtggcg gcggggcgcg gaaaaagagc
     2701 ggcggcaagg cggcgaaagg gcggggaaag ggaaaaacga aaacgcgcaa ggccgcgtag
     2761 cgcggcgcgt ttcgggcatg ttttccgtgg tgggcgcgcg gcgcaccttt aacgcgaagc
     2821 ttacaccggt tggatgaacc ggttccagaa tacggggtct gttcggccca ccattccgaa
     2881 atacccgtgg actgccatag gggaatcccg gaacttgccc tccatcaatc gccaaacgtt
     2941 gaaaatagcg tatgggggtt tttgatgcgc ccgccttcgg acagcttcct attccgcggc
     3001 gcgagttttg gcatcagttc actctccact tcatcctaaa atcgaatgcc gactgtcgct
     3061 cccgcccact ccatcaacgt gcttgtggtt gaagatcacg aggacgattt ccgttatcta
     3121 tcattcctgc tcgggaggaa tagcattggc tcctacaagc tgcaatgggc gccgagttat
     3181 tcggcggggc agaaggcgat tcgcgcgggc gggtttgacg tggcgctctt cgactaccgg
     3241 ctcggcggcg gcacggggat cgatttgttg aaggaagcgc aggcggccgg ttgcgagaag
     3301 ccgatcatcc tgctgacggg ccatgatagc caggaagtcg accaagaagc gtcgcaagcg
     3361 ggcgcggcgg actatctgtg caagacgggg ctcaacggca cggagctcga gcgggcgatt
     3421 cgttatgcga tgcagcaggc ggcgatgacg gcggcgctgc ggcagagcca gcagcagctg
     3481 gagttgttca tgcggagcgt gccgtgcgcg gtgtgcattc gcgacgacgc aggccggctg
     3541 ctgtttcaaa acgatttgtt cgcgcgctat ttcaaaaacg aggat
//
LOCUS       DASPLT010000178         1283 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_268370, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000178 DASPLT010000000
VERSION     DASPLT010000178.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1283)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1283)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1283
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_268370"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1271)
                     /locus_tag="VEA63_01915"
     CDS             complement(<1..1271)
                     /locus_tag="VEA63_01915"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYD82766.1"
                     /translation="MNYAEGAAAAWWPKHESPLFAELEREGKLPPLAERIGPEPLVLE
                     GVEGLGRYGGSWYRLANSERDVLTTQSLLMTPTLMRWSPQGHPIVPFVAKSLEISPDQ
                     REFTFTLRRGMKWSDGAPFTADDILYWWKWEVLYFKQTPRFMMIAGQMGTVEKIDDLR
                     VRFTFPRPNPYFPTCVARTADQFDFLYPAHYLRQFHPEIGDQALIGRMMAALKLGSAV
                     SVYRRMKEWRNPECPRLSPWLYRTYQASAPHTFVRNPYFFAVDSAGHQLPYLDRVVID
                     VKTPSLIPVAASNGELSMQARNILYDDHTLLLGGMKSGDYRVLHWFPATRSLFTIFPV
                     LNRRIDPAHPGTANKAALLNERRFRQALSLAINRRAIIDAIFNGQGEPAQLDGGPLSD
                     FHSPALFKSFTAFEPDRANALLDELGLTRRD"
BASE COUNT          246 a          353 c          463 g          221 t
ORIGIN      
        1 ttgtcgcgac gcgtgaggcc gagttcatcg agcagcgcgt tggcgcggtc gggctcgaag
       61 gcggtgaagc ttttgaacag cgcgggactg tgaaaatcgg agagagggcc gccgtcgagc
      121 tgtgccggct cgccctggcc attgaagatg gcgtcgatga tcgcgcgccg gttgatggcg
      181 agcgagaggg cctggcgaaa gcgtcgctcg ttgagcagcg cggctttgtt cgcggttccg
      241 ggatgggccg gatcgatgcg gcggttgagc accgggaaaa tcgtgaagag cgagcgggtg
      301 gcgggaaacc agtgaagcac gcgatagtcg ccggacttca tgcccccgag cagcagcgtg
      361 tgatcgtcgt agagaatgtt tcgagcctgc atcgagagtt cgccgttgct cgcggcgacg
      421 ggaatcagcg agggcgtttt gacgtcgatg acgacgcgat cgagataggg cagctggtgt
      481 ccggcggaat cgacggcgaa gaaatacgga ttgcgcacga aggtgtgcgg cgcgctcgcc
      541 tgatacgtgc gataaagcca tggcgagagg cgcgggcact ccgggttgcg ccactccttc
      601 atccgccggt aaacggacac cgcgctgccg agcttcagcg cggccatcat tcgaccgatc
      661 aaggcctgat cgccaatctc cgggtgaaac tgacgaaggt agtgcgcggg atagagaaaa
      721 tcgaattggt cggcggtgcg cgcgacgcag gttgggaaat agggattggg ccgcggaaac
      781 gtgaagcgca cgcggaggtc gtcgattttt tcaaccgtgc ccatttggcc agcgatcatc
      841 atgaaccgcg gcgtttgctt gaagtagagc acctcccatt tccaccagta gagaatgtcg
      901 tccgcggtga acggcgcgcc gtccgaccac ttcatcccgc ggcggagcgt gaaggtgaat
      961 tcgcgctgat cgggcgaaat ctcgagcgac ttggcgacga aaggaacgat ggggtgacct
     1021 tgcggcgacc agcgcatgag cgtgggcgtc atgagcagcg actgggtcgt gagcacgtcg
     1081 cgctcggaat tggcgagcct ataccaggaa ccgccgtagc gaccgagccc ctcgacgcct
     1141 tcgaggacca gcggctcggg cccgatgcgt tcggcgagcg gcggaagttt gccctcgcgt
     1201 tcgagttcgg cgaagagcgg cgattcgtgc ttcggccacc acgcggcggc ggcgccctcg
     1261 gcatagttca cgtcgcgaac gac
//
LOCUS       DASPLT010000179         1049 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_124952, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000179 DASPLT010000000
VERSION     DASPLT010000179.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1049)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1049)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1049
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_124952"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1049
                     /locus_tag="VEA63_01920"
     CDS             <1..>1049
                     /locus_tag="VEA63_01920"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HYD82767.1"
                     /translation="ALWRRVVELAPDYTPARLRLGDSLLKNNRLDEAAATYAAVLERD
                     HDNAYAQLGLARIDLEAERWTQARERLETVVRATNYELGYDLIVSLYERLGQADRALA
                     IRGSAKAFGSFRDPVDPWLDALLDLCYDPYRISLAAGIAARSGDPARGRALLERAIEL
                     APGDISTRFQLGTLSLQQGDTTTAREQFERCTILAPDFSDGWAQLSALQAKLGDSAGA
                     ARTLATGLRHCPDSPGLHLMQARTFRQAGRIEEAINEFMISINLRPNEPEAFIELGSM
                     LVSAGRTDEGMEQLRRSLETDPANPMALSILAFHAISTRNEAEADQWMSRIAEQPRVS
                     REQASRIHAAYRQQF"
BASE COUNT          189 a          382 c          307 g          171 t
ORIGIN      
        1 cgcgctctgg cggcgcgtcg tcgaactcgc gcccgactac acgccggcac ggctgcgctt
       61 gggcgacagc ctattgaaaa acaaccggct cgacgaagcc gccgccacct acgccgccgt
      121 gctcgagcgc gaccacgaca atgcctacgc gcaactcggg ctcgcgcgca tcgatttgga
      181 agcggaacgc tggacgcaag cacgcgagcg cctcgagacc gttgtccgcg cgacgaatta
      241 cgaactcggc tacgatctca tcgtcagcct ctatgaacgc ttgggccagg ccgaccgcgc
      301 gctcgcgatc cgcggctcgg ccaaggcctt cggctcattt cgcgatccgg tcgatccgtg
      361 gctagatgcg ctgctcgatc tctgttacga cccctatcgc atctcgctcg ccgcgggcat
      421 cgccgcccgc tccggcgatc ccgcacgcgg ccgggcgctg cttgaacggg ccatcgagct
      481 cgcgcccggc gatatctcga cgcgtttcca actcggcacg ctttcgttgc aacaaggcga
      541 cacgaccacc gcacgcgagc agttcgaacg ctgcacgatt ctcgcgccgg atttttccga
      601 cggctgggcc cagctcagcg ccttgcaggc gaaactcggc gattccgccg gcgccgcgcg
      661 cacgcttgcg accgggctgc ggcattgtcc ggattcaccc gggctgcacc tgatgcaggc
      721 acgcacgttt cgtcaggctg gtcgcatcga ggaagcgatc aacgaattca tgatctcgat
      781 caacctgcga ccgaacgaac ccgaagcatt catcgagctc ggctcgatgc tggtttccgc
      841 cggccgcacc gacgaaggca tggagcaact tcgacgctcg ctcgaaaccg atccggcgaa
      901 tccgatggcg ctcagcattc tggcgtttca cgccatctcg acccgcaacg aagccgaggc
      961 cgaccagtgg atgagccgca ttgccgaaca gccgcgcgtc agccgcgagc aagcttcacg
     1021 catccacgcc gcctaccgcc agcaattcg
//
LOCUS       DASPLT010000180         1287 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_227521, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000180 DASPLT010000000
VERSION     DASPLT010000180.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1287)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1287)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1287
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_227521"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..623
                     /locus_tag="VEA63_01925"
     CDS             <1..623
                     /locus_tag="VEA63_01925"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HYD82768.1"
                     /translation="LPSLGLVSAGVFYKDIENFSYETEVEADPAYPDYEVTSFRNGSK
                     GSILGLELAYQQQFRFLPAPFDGVGLLANVTFSDSEADYPTRPGEDIRFIGQSERVGN
                     VGLSYEKAGFFMRLALNFRSERLREDEPLGGDALTDLWVDDSAQLDLTARYRVTRNWE
                     LFAEFLNLTNEPFRVYQRGGDIGPSKRLVQFEEYDWSANFGVRWNL"
     gene            675..>1287
                     /locus_tag="VEA63_01930"
     CDS             675..>1287
                     /locus_tag="VEA63_01930"
                     /inference="COORDINATES: protein motif:HMM:NF014396.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016158 - 3-phytase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytase"
                     /protein_id="HYD82769.1"
                     /translation="MTSSFPRTRLVVLLTLLLVWCGCDELARPAADAVKPRVITEPTR
                     HDTDDPAIWVNPADPAKSLVLGTDKDTDGALYVYDLDGKIVKAVTGLARPNNVDVAYG
                     FVLGEAKVDIAVVTERERQRLRVFRLPEMESIDGGDLVVFGGDVTRAPMGVALYRRSN
                     DGALFVFVSGKSGPTDGYLAQYRLASDASGKLALTRVREFGRYS"
BASE COUNT          236 a          357 c          422 g          272 t
ORIGIN      
        1 ttcttccctc gctgggattg gtttcggcgg gtgttttcta caaggacatc gagaacttca
       61 gctacgaaac cgaggtcgag gccgatccgg cgtatccgga ttatgaagtc acctcgttcc
      121 gcaacggcag caaggggagt attctcgggc tcgagctcgc gtatcagcag cagtttcgtt
      181 ttctgccggc gccgttcgac ggtgtggggt tgctcgccaa cgtcacgttt tccgacagcg
      241 aagccgacta tccgacgcgt ccaggcgagg acattcgttt catcgggcaa tcggaacgcg
      301 tcggcaatgt ggggctcagc tacgagaagg cgggtttttt catgcgcctc gcattgaact
      361 ttcgttccga acgactgcgc gaagacgaac cgctcggcgg cgatgcgttg actgatctgt
      421 gggtcgacga ttcggcgcag ctcgatctca cggcgcgcta tcgcgtgacg aggaactggg
      481 agctgtttgc ggaattcctc aatctgacga acgagccgtt ccgcgtgtat cagcgcggcg
      541 gtgacatcgg tccaagcaaa cgcctggtgc agttcgagga atacgactgg tcggcgaact
      601 tcggggtgcg gtggaatctg tgagactgac gccaaccgac cgaatcaata ctcaacttag
      661 ctgaaccttt accgatgact tcttcttttc cacggacgcg tcttgtcgtc cttctgacgc
      721 tgctgctcgt ttggtgcggt tgcgacgagc tcgcgcgtcc ggcagcggat gcggtgaagc
      781 cgcgtgtgat cactgagccg acgcgacacg acaccgacga tccggcgatc tgggtcaatc
      841 ccgccgatcc ggcgaaaagc ctcgtgctcg gcaccgacaa ggacacggac ggcgcgctgt
      901 atgtttatga tctcgacgga aaaatcgtga aggctgtcac cggtctcgcg cgaccgaaca
      961 atgtcgatgt ggcctacggc ttcgtgctcg gtgaagcgaa ggtggacatc gcggtcgtca
     1021 ccgaacgcga gcgtcagcgg ctgagggttt tccgcctacc agagatggag agcatcgatg
     1081 gcggggatct ggtggtgttc ggcggcgatg tgacacgcgc gccgatgggc gtggcactgt
     1141 atcggcgttc caatgacggc gcgctgttcg tgttcgtcag cggaaagtcg ggaccgacgg
     1201 acggctatct cgcgcagtat cggctggcct ccgatgcgag cggcaagctg gcgctgacgc
     1261 gagtgcggga gtttggacgc tactcgg
//
LOCUS       DASPLT010000181         1333 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_104147, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000181 DASPLT010000000
VERSION     DASPLT010000181.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1333)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1333)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1333
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_104147"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..221
                     /locus_tag="VEA63_01935"
     CDS             <1..221
                     /locus_tag="VEA63_01935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD82770.1"
                     /translation="GIQMLEHGLVAEPAHPIALSILAFAAIQTQRQELADRCLRQIVE
                     HPRVTSAERTRLFASYENTFGRAFSWAR"
     gene            209..>1333
                     /locus_tag="VEA63_01940"
     CDS             209..>1333
                     /locus_tag="VEA63_01940"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HYD82771.1"
                     /translation="MGSLNHSLRGCCSIGAALALTLAGCDRTTRARPAPATATAEELD
                     RLIPEPVGRRTTGHHAWVSNLIAVDLDRDGRDDVVACEAQDHEVIWLKQNPGGAFEEI
                     VLAKNMRGPVHTAAADMDDDGDLDLLVASMGELFPNNDRIGTIFLLENDGEQTFTPHV
                     VLERVARVTDVQPVDLNGDGRLDLAVAQFGYDQGEVRWMERVGPREFRSHILLNLSGA
                     INIRVADFNQDGRPDLVANLSQQWEEVHLFENTGGGTFRTRTLFGSTNDDFASSNLAI
                     GDVDRDGLTDIVFANGDGFGPTTHPGPRPWHGVQWLHHRGDGKFDFQRLGALAGAYSP
                     IVVDLDQDGHNDIVAVSAFNDWRTPRAISMQWFRNDGNSNF"
BASE COUNT          257 a          450 c          389 g          237 t
ORIGIN      
        1 agggcatcca gatgctcgaa cacggcctcg tcgccgagcc cgcccatccc atcgcgcttt
       61 cgatcctcgc gtttgccgcg atccagacac aacggcagga actcgccgat cgctgtctcc
      121 gtcagatcgt cgagcatcca cgcgtcacct ccgccgaacg cacccggctg ttcgccagct
      181 acgaaaacac gttcggccgc gctttctcat gggctcgctg aatcactcgc tccgcggctg
      241 ttgctcgatc ggcgcagcgc tcgcgctcac cctagccggt tgcgatcgaa cgacacgcgc
      301 acgccccgcg ccggccacag cgaccgcgga agaacttgat cggctcatcc ctgaacccgt
      361 cggccggcgc accaccggcc atcatgcgtg ggtctcgaat ttgatcgcgg tcgacctcga
      421 ccgcgacggt cgcgacgacg tcgttgcctg cgaagcgcag gaccatgaag tgatctggct
      481 gaaacaaaac cccggcggcg cgttcgagga aatcgttctg gcgaaaaaca tgcgcggtcc
      541 cgttcacacg gctgcggccg acatggacga cgatggcgac ctggacctgc tggttgccag
      601 catgggcgag ttgtttccga acaacgaccg gatcggcacg atcttcctcc tcgaaaacga
      661 cggcgagcag acgttcaccc cgcacgttgt gctcgaacgc gtggcacgag tcaccgacgt
      721 ccaacccgtc gatctcaacg gcgacggccg gctcgatctc gccgttgcac aattcggcta
      781 cgaccagggc gaggtccgtt ggatggaacg cgtcggaccg cgggaattcc gcagccacat
      841 cctcctcaat ctttccggcg cgattaatat tcgcgtcgcc gattttaatc aggacggccg
      901 accggacctc gttgcgaacc tctcgcagca atgggaggag gtgcatttat tcgagaacac
      961 cggcggcgga acgtttagaa cgcgcacctt gttcgggtcg acgaacgacg atttcgcgag
     1021 cagcaacctc gcgatcggag atgtggatcg cgatggatta accgacatcg ttttcgccaa
     1081 cggcgacggc ttcggtccca cgacacatcc gggaccgcgt ccgtggcacg gcgtgcagtg
     1141 gctccaccat cgaggcgatg ggaaatttga tttccaacgc ctaggcgcgc tcgccggcgc
     1201 ctacagtccg atcgtggtgg atctcgatca ggacgggcac aacgacatcg tcgccgtcag
     1261 cgccttcaac gactggcgca cgccgcgcgc gatctcgatg cagtggtttc gtaacgacgg
     1321 caactccaac ttc
//
LOCUS       DASPLT010000182         1000 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_24102, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000182 DASPLT010000000
VERSION     DASPLT010000182.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1000)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1000)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1000
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_24102"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..338)
                     /locus_tag="VEA63_01945"
     CDS             complement(<1..338)
                     /locus_tag="VEA63_01945"
                     /inference="COORDINATES: protein motif:HMM:NF014376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family ligand binding domain-containing
                     protein"
                     /protein_id="HYD82772.1"
                     /translation="MRHLVFAQLNGRAREHHLALTRLAPATETFLHDHDFVELFLVTG
                     GAGQHVCNGHASALGRGMLVGVRAVDAHYFRSAADAPLQFINLALAPAWWAQVLKIVA
                     PAVGEAWARA"
     gene            420..>1000
                     /locus_tag="VEA63_01950"
     CDS             420..>1000
                     /locus_tag="VEA63_01950"
                     /inference="COORDINATES: protein motif:HMM:NF017532.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HYD82773.1"
                     /translation="MTASSPTTPATTRGRLTPDQVAHYQREGYLVFNQPVLPEERFHS
                     LKAKFEQILGDLPGDQRPEAMDVPHFMHPELLTWAFDDAILNLVEPIVGPDIALFSTH
                     FICKPKGNGRRVPWHEDSAYWNGKITPMEVCTVWLAIDPSRRENGCMMVIPRTHAEGR
                     AGFSDYDPVDPTKNVFWTEIRASQRDDSKRIAI"
BASE COUNT          209 a          343 c          278 g          170 t
ORIGIN      
        1 ctcgcccgcg cccacgcctc gccgaccgcc ggcgcaacga tcttcaacac ctgcgcccac
       61 catgccggcg cgagcgcgag gttgatgaac tgcagcggcg catccgccgc gctgcgaaaa
      121 tagtgcgcat ccaccgcgcg cacgcccacc agcatcccgc gcccgagcgc cgaagcgtgc
      181 ccgttgcaga catgctgccc cgcaccgccc gtaacgagaa acagctccac gaaatcatga
      241 tcgtgcagaa acgtttccgt cgccggtgcc agtcgcgtga gcgccagatg atgctcacgc
      301 gcgcggccgt tcaactgcgc aaaaaccagg tggcgcatca attaggtgta gttgttcatc
      361 aatgccgtgc aagacgaggc tagcctcggt gcgttaagat aagcgagtag tcacacacca
      421 tgaccgcctc atcccccacg actccagcca ccacccgcgg ccgactcacc cccgaccagg
      481 tcgcgcacta tcagcgcgag ggatacctcg ttttcaatca gccggtgctt ccggaagagc
      541 gctttcattc gctgaaggcg aagttcgaac agatcctcgg cgacctgccc ggcgatcagc
      601 gcccggaagc catggacgtg ccgcatttca tgcaccccga gttactcacg tgggcgttcg
      661 atgatgcgat tctcaacctc gtggagccga tcgtcggacc ggacatcgcg ctgttctcca
      721 ctcatttcat ctgcaaaccg aaaggaaacg gccgccgcgt gccgtggcac gaagactccg
      781 cctattggaa cggcaagatc acgccgatgg aggtctgcac cgtctggctc gcgatcgatc
      841 cttcgcgccg ggaaaacggc tgcatgatgg ttattccgcg cacgcatgcg gaaggccgtg
      901 ctggtttttc cgactacgat cccgtcgatc caacgaaaaa cgtattctgg acggaaatcc
      961 gtgcgagtca gcgcgacgac tcgaagcgca tcgcgatcga
//
LOCUS       DASPLT010000183         1326 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_207669, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000183 DASPLT010000000
VERSION     DASPLT010000183.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1326)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1326)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1326
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_207669"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..393
                     /locus_tag="VEA63_01955"
     CDS             <1..393
                     /locus_tag="VEA63_01955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012377411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding domain-containing protein"
                     /protein_id="HYD82774.1"
                     /translation="VIQLRNNYDKNLFNGDIGTIVSVDPEAGTLDAEFDGARHTFERG
                     EFGDLALAYAISIHKSQGSEYPVVIIPLLKGHFMMLQRNLIYTAITRGRKKVILVGEP
                     AAYAMAVRNSESKQRLTHLREKIAAATA"
     gene            469..>1326
                     /locus_tag="VEA63_01960"
     CDS             469..>1326
                     /locus_tag="VEA63_01960"
                     /inference="COORDINATES: protein motif:HMM:NF017081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MASE1 domain-containing protein"
                     /protein_id="HYD82775.1"
                     /translation="MESITGRRAAVVYLAYFLLHAGFDASARLFEVAPGVSLWYPPVG
                     LALALSTMMGVRAAPAVLAASFYSALLTSTNSESWTRFALPVLITTVYVAAGVLVRHW
                     FGPVPTPQRPLRTAMLVAIYLAAPLLSASIGSALVIAASHLPPGEFFSLSFGWWIGDL
                     TGVLTVVPICLVHIAPALMRQQPAPQRNWTLRERLEIVAQTFTLLAFVGIVHGFGYVH
                     PHQTYYLCFVPLVWICLRHGLPGATIAVLALTMGTLFSLQITGASNIVITDLVLFEIA
                     VATIGLGLGA"
BASE COUNT          210 a          479 c          359 g          278 t
ORIGIN      
        1 gtcatccagc tgcgcaacaa ctacgacaaa aacctcttta acggcgacat cggcaccatc
       61 gtctccgtcg atccggaagc gggcacgctc gacgccgagt tcgacggcgc acgccacacg
      121 ttcgagcgcg gcgagttcgg cgacctcgcg ctcgcctacg ctatcagcat tcacaagtcg
      181 cagggaagtg aatacccggt cgtcatcatc ccactgctca aaggccactt catgatgctg
      241 cagcgaaacc tgatctatac cgcaatcacc cgcggccgaa aaaaagtcat tctcgtcggc
      301 gaacccgccg cctacgcgat ggcggtccgc aatagcgaat cgaaacagcg cctcacgcat
      361 ctccgcgaaa aaatcgccgc cgccaccgcc tgattcactc gcgtttgccg ggccgtttgc
      421 cccttgcact gcccggcgcc gaacactgca atcgcgacca cctgaatcat ggagtccatc
      481 accggccgtc gcgcagccgt cgtttatctc gcttattttc tccttcacgc ggggttcgac
      541 gcgagcgccc gattgttcga ggtcgcaccg ggtgtgtcgc tctggtatcc cccggtgggg
      601 ctcgccctcg cgctcagcac gatgatgggt gtgcgcgccg cacccgcggt cctcgccgcg
      661 agtttttatt cggcgctgct caccagcacg aactcggaga gttggacgcg cttcgcgctg
      721 ccggtgctga tcaccacggt ttacgtcgcg gccggtgtgt tggtgcggca ctggttcggg
      781 cccgtgccaa cgcctcaacg tccgctgcgc acggccatgc tcgtcgcgat ctatctcgcc
      841 gcgcctttgt tgtccgcctc catcggttca gcgttggtca tcgccgcgtc ccatcttccg
      901 cccggcgaat ttttctccct cagcttcggc tggtggatcg gcgacctcac cggcgtgctc
      961 accgtcgtcc ccatctgcct cgttcacatc gctcccgcgc tcatgcgtca acaacccgcg
     1021 ccgcagcgaa actggacgct gcgcgaacgg ctcgagatcg tcgcgcaaac tttcacgctg
     1081 ctcgcgtttg tcggcatcgt ccatggcttc ggctacgttc atccgcacca gacctattat
     1141 ttgtgcttcg ttcccctcgt ctggatctgc cttcgccacg ggctgcccgg cgcgacgatc
     1201 gccgtcctcg ccctcacgat gggcacgctc ttttcgctgc agatcacggg cgcatcgaac
     1261 atcgttatca ctgacctcgt gctcttcgag atcgcggtgg ccacgatcgg tctcgggctc
     1321 ggcgcc
//
LOCUS       DASPLT010000184         3990 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_145997, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000184 DASPLT010000000
VERSION     DASPLT010000184.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 3990)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3990)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3990
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_145997"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>3990
                     /locus_tag="VEA63_01965"
     CDS             <1..>3990
                     /locus_tag="VEA63_01965"
                     /inference="COORDINATES: protein motif:HMM:NF037015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HYD82776.1"
                     /translation="LPLSIAISAEAPLAISRNGEKSFSISGRFNPGSLFEILVDGALI
                     DTAVYNGEALPFAAVAADTIGRSEGASVSIAVRLKDASGEELLSNALQLTVGSPLTVV
                     PLVPTGTANAPTIAVNSLTAGTSFLEHAALTIAGTVADADGDAGRVRVLIGATELTGA
                     DVAPNGTFSTQVYLPNVTEFTPVTVSVYAVDAAGNVSEPNRTAINLVNDSEKPIVAGI
                     LADAAQTAVLAGVDLHLRPSVTDNVGIALLVTQVSLDGTVVRTIVNRYRHNASSREQA
                     IGQRIDPVTMMGRSLVVTTTATDFAGNSTVATAKVDVLSVYHLAQSKAGRPLPGVITQ
                     LAQSGGTLFGVFTLARNATVSESFVFSADEPAGGELVVRDVIATESVISSLIAHAGQL
                     YAFTEDRSLLRVDIANPAALALVGITDVPAAFTSVASMSDGLVAAGEGKLAVFDLSRH
                     DNPVLEKIASIACQSVEARGALIYASNASNVQVLNRHLETIKTYAALGAADLLLQREG
                     LAVASGSSVRNVVDGFGLATRVDNQLLVGPAWNTLSPVHGIAALDLRLLIAATSQGVE
                     FARASGQAGWGRVASTAQPAAAREVVSGRLGRYYVRHDAGYLSWLDVDASGANLPSSA
                     AVSLTGEALDVEIVGSLAIVAKGQNGLSLVDLGRPESPKLIRNVTTPGSVVSLAHRGN
                     RLYLGLGGAGILQTSISQLGTAVTGVPVAVQGGVQKVIAADSSLVAMGGGVAVLDPVS
                     LAVLSPITMAGAVRDVSASGSLLAVATAAHGVKVFDLSDPTHPIVVATIAAPAGEITT
                     VYLTGGSLYLGRWDPSRPQPAFIEQYGDGTVALDQFVRQLNASPLPSKINALGRWGNR
                     IVAACGNAGVILIDAVNGAVTQLDSPDFALSTESAETALFVADSFGGMQAFYRDPAAD
                     SPALEVDAPAQRFDIAKGGERLISFAASSLAGVSRVSVAVAGAPAVVFNEPPFAYNLR
                     VPANAPPGSLIEVNASVETISGVQRALSHPIVARVVEAGSGDIVAPTVVLGALPASVA
                     EGGVLHLTATASDAGSIPSVSFFVDGVLVAVDSSYPYAADVNVPSIEGSRPVTVRAQV
                     EDGAGNVGFDERVIAATDDAAAPAVPVFIAPSPTGARPVVEATTLVLRASASDAVAVW
                     KVQLMVNGALYAESTSSPYDFQFPLPLVSGDTPLVLSLVAWDYAGHSTPSAPLNLTIV
                     KYQPPTVAIASIVPVRNFVEGTQINVTVNAASVIGVAEVDLFLGEENETAVATRPGNE
                     AGVYAFTVVLPQIEGLSEELELHARVRDLRLVETMSAPASVTVVEDKPPTGSLLVVPA
                     GSDFYEKTNV"
BASE COUNT          631 a         1199 c         1364 g          796 t
ORIGIN      
        1 ctgccgcttt ctattgcgat cagcgcggag gcgccgctcg cgatttcgcg gaacggcgag
       61 aagtcattct cgatcagcgg gcgcttcaat ccgggttcgc tcttcgagat tcttgtcgat
      121 ggagcgctca tcgacaccgc cgtctacaac ggcgaagcgt tacctttcgc tgcggtggcg
      181 gctgacacga ttggcagatc ggaaggcgcc agcgtttcga ttgccgtgcg tttgaaggat
      241 gcctcgggcg aggaattgct gtcgaacgcc cttcagttga cggtgggttc gccgctgacg
      301 gtggttccgc tggtcccgac cgggacggcg aacgcgccaa ccatcgcggt gaatagtttg
      361 acggcaggca cgtcatttct cgagcacgcg gcgttgacga tcgcgggcac ggtcgccgat
      421 gccgatggcg atgcgggacg agttcgagtc ttgattggcg cgaccgagct aaccggcgcc
      481 gatgtcgcgc ccaacggcac gttcagcacg caggtttacc tccccaacgt caccgagttc
      541 acgccggtga cggtgtcggt ttatgcggtc gacgcggccg gaaatgtttc cgagccgaac
      601 cgaacggcga tcaatctcgt gaacgattcg gagaagccca tcgtcgcggg aattctggcg
      661 gatgcggcgc agaccgcggt cctcgccggg gtcgatcttc acctgcggcc gtcggtcacg
      721 gacaatgtag gcatcgcctt gctcgtcacg caggtctcgc tcgatggcac ggtcgttcgg
      781 acaatcgtga atcgctaccg ccacaacgcg agcagccgtg aacaggcgat tgggcaacgc
      841 atcgatccag tcacgatgat gggacgttcg ctggtcgtca cgacgacggc aaccgatttt
      901 gcgggcaact ccacggtggc gacggcgaag gtcgatgtgc tgtcggtgta tcatctagcg
      961 cagtcgaagg ccggccggcc gctgccgggc gtgatcactc agcttgcgca aagcggcggc
     1021 acgctcttcg gcgtgttcac cctcgcgcgt aatgcgacgg tttctgagag cttcgttttt
     1081 tcggccgatg agccggcggg cggcgaactg gtcgttcgcg atgtaattgc aaccgagtcg
     1141 gtgatctcgt cgctgatcgc gcacgccggg caactctacg cgttcacgga ggatcgctcg
     1201 ctgttgcgcg tcgatatcgc taatccggcg gctttggcgc tcgtcggaat caccgacgtg
     1261 ccggcggcgt tcacttccgt tgcgtcgatg tcggacggtc tcgttgcggc gggcgagggg
     1321 aagctggcgg ttttcgatct gagccgccac gacaaccccg tgctggaaaa aatcgcatcg
     1381 atcgcctgcc agtcggtgga ggcgcgcggg gcactgatct acgcgtcgaa tgccagtaac
     1441 gtccaggtgc tcaaccgcca ccttgagacg atcaaaacct atgccgcgtt gggggctgcc
     1501 gatttgttgc tgcagcgcga gggtttggcc gtggcgagcg gaagtagcgt gaggaacgtg
     1561 gtcgatggat tcgggctggc cacgcgggtc gacaatcaac tgctggtggg accggcatgg
     1621 aataccctgt cgccggttca cggcatcgcg gcgctggatt tacgcctgct catcgcagcg
     1681 acgtcgcaag gagttgagtt cgcccgtgct tcgggccagg ccgggtgggg gagagtggcg
     1741 tcgaccgcgc agccggccgc cgcgcgggaa gtcgtgagcg gacggctcgg gcggtattac
     1801 gttcggcacg acgccggcta tttgtcttgg ctggacgtcg atgcgagtgg cgcgaatttg
     1861 ccctcaagtg cggcggtgag cctgaccggc gaagcgctcg acgtggagat cgtcggcagc
     1921 ctcgccatcg tcgcgaaggg acaaaatggt ttgtcgctcg tcgatcttgg acgaccggaa
     1981 agcccgaagc tgattcgcaa tgtgacgact cctggtagcg tcgtgtcgct cgctcatcgc
     2041 ggcaaccggc tctatctcgg actgggcgga gctggcatcc tccagacgtc gatcagccag
     2101 ttgggcactg cggtaacggg tgttccagtc gcggtccaag gcggcgtgca aaaggtgatc
     2161 gcggccgaca gcagtctcgt cgcgatggga ggcggtgtcg ctgtgttgga tcctgtttcg
     2221 ctggccgtgc tgagtccgat cacgatggcg ggtgcggtgc gcgatgtctc cgcgagcggc
     2281 tcgttgctgg ccgtcgccac cgcggcgcat ggtgtgaagg ttttcgatct ttccgatccg
     2341 acgcacccga tcgtcgtcgc gacgatcgcc gcgcccgctg gagaaatcac cactgtttat
     2401 ctgaccggcg gctccctgta tctcggccgc tgggacccca gccggccgca gcctgcgttc
     2461 atcgagcaat acggcgacgg cacggtcgcg ctcgaccaat tcgtgcggca gctcaacgcg
     2521 agtccgctcc cgtcgaaaat caatgcgctg ggccgctggg gcaaccgcat cgtcgccgcg
     2581 tgcggcaacg ccggtgtgat cctgatcgat gcggtgaacg gtgcggtcac ccagctcgat
     2641 tcaccggatt ttgcgctctc gacggagagt gcggagacgg cgctttttgt ggccgattcc
     2701 ttcggcggga tgcaggcgtt ttatcgtgat ccggcagctg attcaccggc gttggaggtc
     2761 gatgctccgg cgcagcgctt cgacatcgcg aagggcgggg agcgcttgat ttcgtttgcg
     2821 gcgtcgagtc tcgcgggcgt ctcgcgcgtt tcggtcgcgg tcgccggcgc gccagcggtg
     2881 gtgttcaacg aaccgccgtt tgcctacaat ctccgcgtgc cggccaatgc gccgcccggt
     2941 tcgttgatcg aggtgaacgc gagtgtcgaa acgatctccg gcgtgcaacg tgcgctgtcg
     3001 catccgatcg tggctcgcgt ggtcgaggcg ggttccggcg atatcgtggc tccgaccgtg
     3061 gtgctcggcg cgctgcccgc gagtgtggcg gagggcggag tgcttcatct gaccgcgacc
     3121 gcgtcggatg ccggatcgat tccgagcgtg tcgttctttg tcgatggcgt gctggtggcg
     3181 gtcgactcct cttatcccta cgcggcggac gtgaacgtgc cgtcgatcga gggaagccgg
     3241 ccggtgacgg tccgggcgca ggtcgaagat ggcgcaggca atgtcgggtt cgacgaacgg
     3301 gtgatcgctg cgacggacga cgctgcggcg ccggcggtcc cggtgtttat tgcaccgtct
     3361 ccgactggcg cgcggccggt ggttgaagca acgacgctcg tcctccgggc cagcgccagt
     3421 gacgccgtcg ctgtttggaa agtccaactc atggtcaacg gtgcgcttta tgccgagagc
     3481 acaagctccc cctatgactt ccagttcccg ctgccactcg tgtcggggga tacgccgctg
     3541 gttctgtcac tcgtcgcctg ggattacgcc gggcacagca cgccttcggc gccgctgaac
     3601 ctcacgatcg tcaaatatca gccgcccact gtggcgattg cgagcatcgt gcccgttcgc
     3661 aattttgtcg aaggcacgca gatcaatgtg accgtcaacg ccgcctccgt gatcggcgtt
     3721 gccgaggtcg atcttttcct cggcgaagaa aacgaaaccg cggtcgcgac acggcccggc
     3781 aacgaagccg gcgtgtatgc gtttacggtc gtgctgccgc agatcgaagg actttcggag
     3841 gaactggaac tgcacgcgcg ggtgcgcgat ctccggttgg tcgagacaat gtccgctccg
     3901 gcgagcgtca cggtcgtcga ggacaagccg ccgaccggct ccttgctggt cgtgccggct
     3961 ggttccgact tctacgagaa gacgaacgtg
//
LOCUS       DASPLT010000185         1357 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_268499, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000185 DASPLT010000000
VERSION     DASPLT010000185.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1357)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1357)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1357
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_268499"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(101..>1357)
                     /locus_tag="VEA63_01970"
     CDS             complement(101..>1357)
                     /locus_tag="VEA63_01970"
                     /EC_number="1.1.5.-"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016901 - oxidoreductase activity, acting
                     on the CH-OH group of donors, quinone or similar compound
                     as acceptor [Evidence IEA];
                     GO_function: GO:0070968 - pyrroloquinoline quinone binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HYD82777.1"
                     /translation="AIALACLPMATLAQQPEPTPAFPGQTDAPRPATASSGYKVETLT
                     DQLTGPWALAFLPSGNFLVTERPGTMRVVTPDGQVSEPLANVPPVKRVAAEGLHDVVL
                     DPDFATNRTIYFIYFAPPKGEEAAEWPITHFYEDVWTKSLAERRTLDLGTERIGKARL
                     SEDERALENVEVLYEPGVERRLVWAPDGTLFAIGADAFRFYDSELDGIEGRDFRDEPD
                     ITRNFSGRVTRINKDGSIPKDNPWLGRATVSRETYAHGLKDPEGGAIHPDTGALWVID
                     HGPQGGDEINIIKPGADYGWPNVTYGVQYDARQADGRTNVPVGPGLTAAPGVEQPIYY
                     WVPSIAPSGMAFYTGDLFPEWKGDLFVGAMAGRALHRLELDGERVVSEERLLVELDER
                     IREVRDGPDGAIYIFAGNSLMRILPE"
BASE COUNT          236 a          419 c          445 g          257 t
ORIGIN      
        1 acgacactgg attcccgctt tcgcgggaat gacgaagctc ttcgtagcgg ccgcattctc
       61 ggggcaggct cgcaaacggt ggcaaatgaa aacgcgttga ttactccggc aagatacgca
      121 tcaaactatt cccggcaaat atataaatcg ccccatccgg cccatcgcgc acttcacgta
      181 tgcgctcatc gagctcgacc agcaaacgct cctcgctaac gacgcgctcg ccatcaagtt
      241 cgagacggtg cagcgcccgt cccgccatcg caccgacgaa cagatcgcct ttccactccg
      301 gaaacaggtc gccggtatag aaggccatgc ccgacggcgc aatcgacggc acccagtagt
      361 agatcggttg ttcgacaccg ggtgccgcgg tcagtcccgg accgaccggc acattcgtac
      421 gaccatcggc ctggcgcgca tcgtattgca cgccgtacgt gacgttgggc cagccgtaat
      481 cggcgccggg cttgatgatg ttgatttcgt cgccgccctg cgggccatga tcgatcaccc
      541 acagcgcgcc ggtgtccgga tggatcgcgc cgccttcggg atctttcaag ccgtgcgcgt
      601 aggtctcgcg gctgacggtg gcgcggccga gccagggatt gtctttcgga atcgagccgt
      661 ccttgttgat gcgcgtgacg cgaccactga agttgcgcgt gatgtcgggc tcgtcgcgga
      721 agtcgcggcc ttcgatgccg tcgagttctg aatcgtagaa gcgaaacgca tcggcgccga
      781 ttgcgaacag cgtgccgtcg ggcgcccaca cgaggcggcg ttcgacgccg ggttcgtaca
      841 gcacctcgac gttttcgagc gcgcgctcgt cttcactcag acgcgccttg ccgatgcgct
      901 cggtgccgag gtcgagcgtg cggcgttcgg caagcgattt cgtccacacg tcttcgtaga
      961 aatgcgtgat tggccattcg gcggcttctt cacctttcgg cggcgcgaag tagatgaagt
     1021 aaatggtgcg gttcgtcgcg aagtcgggat cgagcacgac gtcgtgcaag ccttccgcgg
     1081 cgacgcgttt gaccggcggc acgttcgcga gcggctcgga aacctggcca tctggcgtca
     1141 cgacccgcat cgtgccgggg cgttcggtga cgaggaagtt gccgctcggc aggaacgcga
     1201 gcgcccaggg tcccgtcagc tggtcggtga gcgtttctac cttgtaaccg gatgaagctg
     1261 tcgcaggacg cggcgcatcg gtctggccgg gaaaggcggg cgtcggttcc ggttgctgcg
     1321 cgagtgtggc catggggagg caggcgaggg cgatggc
//
LOCUS       DASPLT010000186         1150 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_207690, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000186 DASPLT010000000
VERSION     DASPLT010000186.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1150)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1150)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1150
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_207690"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1150)
                     /gene="glnS"
                     /locus_tag="VEA63_01975"
     CDS             complement(<1..>1150)
                     /gene="glnS"
                     /locus_tag="VEA63_01975"
                     /EC_number="6.1.1.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR00440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glutamine--tRNA ligase"
                     /protein_id="HYD82778.1"
                     /translation="VITRFPPEPNGYLHIGHAKSICLNFGIALENGEQARCHLRFDDT
                     NPETEDVEYVNSIMNDVKWLGFDWGEHLYYASDYFDWMYEFAELLIKNGHAYVDSLSA
                     EEMRAYRGTLTEPGKDSPYRTRSAEENLDLFRRMRAGEFREGEHILRAKIDMASPNIN
                     MRDPAIYRIRHAAHHRTGDKWCIYPMYTYAHPIEDALENITHSLCTLEFEDQRPFYEW
                     LLNRIADLGGLLRPLPTQTEFARLNLTYVALSKRKLIQLVQEGHVDGWDDPRMATIVG
                     FRRRGFTPESIRLFCDRIGVSKADSWIDMTVLEDCLREDLNERALRRIAVLDPLKLVI
                     ENYPEGQSEECYAPNHPQKPELGKRVVPFSRELWIEREDFMEVPPKGY"
BASE COUNT          206 a          333 c          371 g          240 t
ORIGIN      
        1 aaatagccct tgggcgggac ttccatgaaa tcttcgcgct cgatccacag ttcgcgcgag
       61 aacggcacca cgcgcttgcc gagttccggc ttctgcggat ggttcggcgc gtaacactct
      121 tcgctctggc cttcgggata gttctcgatg acgagcttga gcggatcgag cacggcgatg
      181 cggcgcagcg cgcgttcgtt caggtcttcg cggaggcaat cctcgagcac ggtcatgtcg
      241 atccacgaat cggccttcga cacgccgatg cgatcgcaga acaggcggat ggattcgggc
      301 gtgaaaccgc ggcgacggaa cccgacgatg gtcgccatgc gcggatcgtc ccagccgtcg
      361 acgtggcctt cctgcacaag ttggatcagc ttgcgcttcg agagtgcgac ataggtgaga
      421 ttcagccgcg cgaattccgt ttgcgtgggc agcggacgca gcaggccgcc gagatcggcg
      481 atgcggttca acagccactc gtagaacggc cgctgatcct cgaactccag cgtgcacagc
      541 gaatgggtga tgttctcaag cgcgtcctcg atcggatgcg cgtaggtgta catcggatag
      601 atgcaccact tgtcgccggt gcggtgatgc gcggcgtggc ggatacggta gatcgccgga
      661 tcgcgcatgt tgatattggg cgacgccatg tcgatcttcg cgcgcaggat gtgctcgcct
      721 tccctgaact cgcccgcgcg catgcggcgg aagagatcga ggttttcctc ggcgctgcgc
      781 gtgcgatacg ggctgtcctt gcccggttcg gtcagcgtgc cgcggtacgc gcgcatctct
      841 tcggcggaaa gactgtcgac gtacgcgtga ccgttcttga tgagcagttc ggcgaattcg
      901 tacatccagt cgaaatagtc cgaggcgtag tagaggtgtt cgccccagtc gaagccgagc
      961 cacttcacgt cgttcatgat cgagttgacg tattcgacgt cttcggtttc cggattcgtg
     1021 tcgtcgaagc gcaggtggca acgcgcctgc tcaccattct cgagcgcgat tccgaagttg
     1081 agacagatcg atttggcgtg acctatgtgc agatacccgt tcggctccgg cggaaaacgc
     1141 gtgatgacgc
//
LOCUS       DASPLT010000187         1083 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_146018, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000187 DASPLT010000000
VERSION     DASPLT010000187.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1083)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1083)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1083
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_146018"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(125..808)
                     /locus_tag="VEA63_01980"
     CDS             complement(125..808)
                     /locus_tag="VEA63_01980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HYD82779.1"
                     /translation="MRILVIEDEAQLARHVVRALERRGHLAGMRHDGVDGLQSALADP
                     PDLVVLDLNLPNLDGMTVLGRLREAKNPARVLILTARGEVEQRVRGLNAGADDYLAKP
                     FSLDELVARVEALGRRGATPTAEDFLKVGDLQMDVQHRRVSRAARPIALSPREFDVLQ
                     VLMQEPGRVFSRTELCERVWQRDHEYDTRTVEIFIARLRKKVDTGFPVPLIHTIRSVG
                     YTVKVPAAV"
     gene            complement(910..1068)
                     /locus_tag="VEA63_01985"
     CDS             complement(910..1068)
                     /locus_tag="VEA63_01985"
                     /inference="COORDINATES: protein motif:HMM:NF025452.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4266 domain-containing protein"
                     /protein_id="HYD82780.1"
                     /translation="MRVQPWQRAALTDYGMRPDRDPLASASTDHTYFSREAAHGGRGV
                     GGSGCGCN"
BASE COUNT          208 a          381 c          316 g          178 t
ORIGIN      
        1 ccttcgatca ccgacgctgc gtgaagatcc aacgttaaca gcaccagcag tggcacgcgg
       61 ccattccgcc caagttttcg tgcccaacca cggcagctcg tcgccaccct tgtcgaaagc
      121 cacttcatac cgcggcggga accttaaccg tgtagccgac agaacggata gtgtggatca
      181 ggggcacggg aaagccggta tccaccttct tccgcagccg cgcgatgaaa atctccacgg
      241 tgcgcgtgtc gtattcgtga tcgcgctgcc agacgcgttc gcacaattcg gtgcgcgaaa
      301 acacgcggcc gggctcctgc atcaacacct gcagcacatc gaactcgcgc ggggaaagtg
      361 cgattggacg cgccgcgcgg ctcacgcgac gatgctgcac gtccatctgc agatccccga
      421 ctttcaagaa gtcttccgca gtcggcgtcg ccccgcggcg ccccaacgcc tccacgcgcg
      481 cgacgagctc atcgagcgag aagggtttag ccaggtagtc gtccgctccc gcgttcaatc
      541 cgcgaacgcg ctgctcgact tcaccgcgag ctgtcaggat gaggacgcgc gcggggttct
      601 tcgcctcgcg caagcggccg agcaccgtca tgccgtccaa gttcggcaga ttgagatcga
      661 gcacgaccag atccggcgga tccgccaagg cgctctgcag accgtcaacg ccgtcgtgtc
      721 tcatacctgc gagatgtcca cgacgctcga gcgctcgcac gacatgccgc gcaagttgcg
      781 cctcatcttc gatcacaagg atccgcatgg ctcatccgtg atacacgttc cgccgcgctt
      841 gtcagcccgt tgcgcgagaa tcagggaaag cacgaaaagc tcatcgcact gcgaagaggt
      901 gccgcgccct caattgcagc cgcagccgct gccacccacg ccgcggccgc catgcgccgc
      961 ttcgcgcgag aaataggtgt ggtccgtcga ggcgcttgcc agcgggtcgc gatccggccg
     1021 cataccataa tcggtgagtg ccgcgcgttg ccacggctgc acccgcaccg tgccgcaacc
     1081 gcc
//
LOCUS       DASPLT010000188         1188 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_207692, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000188 DASPLT010000000
VERSION     DASPLT010000188.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1188)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1188)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1188
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_207692"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..712
                     /locus_tag="VEA63_01990"
     CDS             <1..712
                     /locus_tag="VEA63_01990"
                     /inference="COORDINATES: protein motif:HMM:NF027666.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HYD82781.1"
                     /translation="EVPDYGAYEKESWADPAKGQAAMITRMDQGVGGVLRRLRELGIE
                     RDTLVIFTSDNGPHREGGPGYDAEFFDANGPWRGIKRDLTEGGIRVPFIARWRGAIPA
                     GAVSAVVGHFADFLPTAAELGDSESSADTDGISLAAVLRGEAAQLRPREHLYWELHEP
                     TFHQAVLLDGRWKAFRGRAGEAAFELYDLANDAMEERDVSQDHPVLVERAIRVMDEAH
                     VPNAWWRNPRKVADSASP"
     gene            complement(762..>1188)
                     /locus_tag="VEA63_01995"
     CDS             complement(762..>1188)
                     /locus_tag="VEA63_01995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD82782.1"
                     /translation="FPTLLELAGISAEPAASLDGISFASALRTGSTPPREALYWHYPH
                     YHPAGLGPSGAVLSGDWKLIESFEAGLTGQPDVCELFNLREAPGEKNNLSIAEPARTA
                     ALRAQLAAWRKSVQAQMPTLNPRYDPARAGELESVSAFH"
BASE COUNT          211 a          316 c          454 g          207 t
ORIGIN      
        1 ggaggtgccg gattacggtg cctacgagaa ggaatcttgg gcggacccgg cgaaggggca
       61 ggcggcgatg atcacgcgaa tggatcaggg cgtgggcgga gtgctgcgac gattgcgcga
      121 acttgggatc gagcgcgaca cgctcgtgat ttttaccagc gacaacggtc cgcatcgtga
      181 gggaggccct ggttacgatg ccgaattttt cgatgcgaac ggcccgtggc gcggcatcaa
      241 gcgcgatctc acggaaggcg gcattcgcgt gccgtttatc gcccgctggc ggggtgcgat
      301 tcccgcgggt gctgtatccg cggtggtagg acactttgcc gacttcctgc cgacggcggc
      361 agagctgggt gacagcgaat cgtcggcgga cacggatgga atcagtctcg cggcggtgtt
      421 gcgaggcgag gcggcgcaat tacggccgcg ggaacatttg tattgggaat tgcatgagcc
      481 gacttttcac caagccgtgc tgctggacgg acggtggaag gcgtttcgcg ggcgcgcggg
      541 tgaggccgcg ttcgagctgt atgatcttgc gaacgacgcc atggaagaac gcgacgtgag
      601 ccaggaccat ccggttttgg tcgagcgggc gatccgcgtc atggacgagg cgcacgtgcc
      661 caacgcgtgg tggcgaaatc cgcgaaaggt cgcggactcc gcttcgccgt gagcgcgagg
      721 aggcttcttg gtcggttgcg cgggcgccgg ccgacggcca tttaatggaa cgcggaaacg
      781 gactccaatt ctccagcgcg ggcgggatcg tagcgcggat tgagggtcgg catctgcgcc
      841 tgcaccgatt tgcgccaggc ggccaattgc gcacgcaatg cggcggtgcg cgcgggctcc
      901 gcgatcgaga ggttgttttt ttcgcccggg gcttcgcgca agttgaagag ttcgcatacg
      961 tccggctggc cggtgaggcc ggcctcgaac gattcgatca gcttccaatc gccggacagg
     1021 acggcgccgg acgggccgag gccggcggga tgatagtgcg gatagtgcca ataaagggcc
     1081 tcgcgcggag gtgtggagcc ggtgcgaaga gcggaggcga aactgattcc gtcgagtgaa
     1141 gcggcgggct cggcggaaat gccggcgagt tcgagcaacg tggggaaa
//
LOCUS       DASPLT010000189         1476 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_248221, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000189 DASPLT010000000
VERSION     DASPLT010000189.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1476)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1476)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1476
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_248221"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..404
                     /locus_tag="VEA63_02000"
     CDS             <1..404
                     /locus_tag="VEA63_02000"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HYD82783.1"
                     /translation="QTRTSVSATPWQQRGKILVIDDEAPVREVASQLIQTFGFSVVTA
                     VDGADGIAQFSRDPTGFALVFLDLTMPGLDGEETLAALRAISPNVRVMLVSGYSENDR
                     VANLATRGPLLFLQKPFTRARLEQKLREILS"
     gene            543..>1476
                     /locus_tag="VEA63_02005"
     CDS             543..>1476
                     /locus_tag="VEA63_02005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009511918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HYD82784.1"
                     /translation="MPGTAFVAPFQVPSSAVTPPASRPLSLGVIFLTLYIDLIGFSII
                     FPLGPDLLTHYLQTDGQSGTLGWLLAQTNLVAHWLGKDSNFAAVLFGGVISSFFSILQ
                     FIFAPFWGAVSDRRGRRGVLLLTVAGTAFGYLLWIVSGSFWLFLLSRIVSGGFSGNLS
                     VATAAVADVTSRSERSKAMGLVGAAFGLGLVTGPTLGALTVHFNLLGRYPELARFGIN
                     PFSVPALVAFGLCLVNLVWIAFRFKETLSASDRAESREPRLRNPITAILHLEDRAVRR
                     TNLVAFVYSVAFVAMETSLTFLAAERFGYTARENG"
BASE COUNT          219 a          535 c          389 g          333 t
ORIGIN      
        1 ctcaaacgcg aacgagcgtt tccgcgacgc cgtggcagca acgcggaaaa atcctggtaa
       61 ttgacgacga agcgcccgtc cgcgaagtcg cctctcagct catccagacg tttggatttt
      121 ccgtggtcac cgccgtggac ggcgccgacg gcatcgcgca gttcagccgc gatccgacgg
      181 gtttcgctct cgtgtttctc gatctcacca tgccgggcct cgacggtgag gaaacgctcg
      241 ccgcgctccg tgcgatctcg cccaatgtgc gcgtgatgct cgtcagcggt tacagcgaaa
      301 acgatcgcgt cgccaacctc gcgacgcgtg gcccactcct gtttctgcaa aaacccttca
      361 cccgcgcgcg gctcgaacaa aaactccgcg agattctgag ttgagcgagc agcaagtgga
      421 gcccgacgtc ctcgtcgggc cgttcatcgt gcgccttcaa accaacccga cgaggactta
      481 gggctccatc tttccggccc gcgcgcgcct cttccggaaa cttcgctttg cgccttggcg
      541 tcatgccggg aaccgctttc gttgcccctt ttcaagtgcc atcatccgcc gtcacccctc
      601 ccgcgtcgcg tccgctctcg ctcggcgtta tttttctcac gctctacatc gacctcattg
      661 gtttctcgat catctttccg ctggggcccg atctgctgac gcattacctg cagaccgacg
      721 gacaatccgg cacgctcggc tggctgctcg cccaaaccaa tctcgtcgcg cactggctcg
      781 gcaaggacag caacttcgcc gccgttctct tcggcggtgt catcagttct ttcttttcga
      841 tcctgcaatt tatcttcgcc ccgttttggg gcgcggtgag cgatcgtcgc ggccggcgcg
      901 gcgttcttct gctcaccgtc gcgggcaccg cgttcggtta tctgctctgg attgtcagcg
      961 gttcgttctg gctgtttctc ctctcgcgca tcgtcagtgg cggcttcagc ggcaatctct
     1021 ccgtcgccac cgccgctgtc gccgacgtca cctcgcgcag cgaacgctcg aaagccatgg
     1081 gactcgtcgg cgccgcgttc ggcctcggcc tcgtcacggg cccgacgctc ggcgcgctca
     1141 ccgttcattt caaccttctc ggccgctacc ccgagctcgc ccgcttcggt ataaatccct
     1201 tcagcgtgcc cgcgctcgtt gctttcggac tttgtctcgt caacctcgtc tggatcgcgt
     1261 ttcgcttcaa ggaaacgctc tccgccagcg atcgcgcgga gtcccgcgaa ccgcgcctcc
     1321 gcaatcccat caccgcaatt cttcacctcg aggatcgcgc cgtccgtcgc accaatctcg
     1381 tcgcgtttgt ctactccgtc gccttcgtcg caatggaaac gtcgctcacc ttcctcgccg
     1441 ccgaacgttt cggttacacc gcgcgcgaaa acggtt
//
LOCUS       DASPLT010000190         1885 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_248260, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000190 DASPLT010000000
VERSION     DASPLT010000190.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1885)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1885)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1885
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_248260"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1885
                     /locus_tag="VEA63_02010"
     CDS             <1..>1885
                     /locus_tag="VEA63_02010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD82785.1"
                     /translation="ATIREVYREYDLEVLEILGGYAPRLPFLGEVENSPFPTNYHEVY
                     RSWRTIAEKFKHTWGAEETWNEPDLVPVPADQFTSLAKVAAAAMSDAGVTVSGGGAFA
                     SVPPSAFFDTCAANGFLDHVDYVSFHTYSPAIDLPAIVTRYRRWLGGVRKDALPLWLT
                     ESGQPWSIGPSRPPLHEDALSAMEIAMKGIEAKACGVARYFPFVYVYYEEGGIKNFGM
                     LGHEATPLRSMAAYVQSIRALSSLEYLGDIESKPDGIKVGRFFGRRDERAPHVLVLYT
                     GQIDGSTVVKLPFRAARAEGMDGRVLPLESDGSMMIPDGLAYLWIDRATAETLIKTDT
                     SVARLHLLGKQSQVAPAERSKVILRYEQEHTPSQRSAQRYLLKKEVARNLPISVRIEN
                     LDKADRRITAQLRLPGVGEAATWETKTIDVPAHGFARLAWRGDASNHLDTVKVSFITI
                     SATSDRSVPISPLVLPFVVEGSLEAYLAGHAKKERLPAADLARWRTNVAPNGKMKLSA
                     SPEAWRMDVAFDGGGSWAFPQFELPSHCDLETATGIVLRARILHPARHVTFYAQINER
                     ERFQVADVFPADGEWHSVFIPFSELVPSPGHVDMQNARLDPKRIAQLSLGFVANGLSG
                     ALEI"
BASE COUNT          372 a          553 c          601 g          359 t
ORIGIN      
        1 gcgacgattc gggaggttta tcgcgaatat gacctcgaag tgcttgagat cctcggcggc
       61 tacgcgcctc ggctgccatt tttgggcgag gtggaaaact cgccgtttcc gacgaattat
      121 cacgaggtgt atcgctcctg gcgcacgatt gcagaaaagt tcaagcacac gtggggcgcg
      181 gaggagacct ggaacgagcc tgatctggtg ccggtgcccg ccgatcagtt cacgagcctg
      241 gcgaaagtgg cggccgcagc catgagcgat gcaggcgtga cagtgtcggg gggaggagcg
      301 tttgcgtcgg tgccgccgag cgcgttcttc gacacatgcg cggcgaacgg atttctcgac
      361 cacgtggact acgttagctt tcacacctac agccccgcga tcgatctccc ggcgatcgta
      421 acgcgctacc gcagatggct cggcggcgtt cgcaaggacg cgctaccgct atggctcacg
      481 gagagcggac aaccgtggtc gatcgggccg tctcgtcctc cgctccacga ggacgccctg
      541 agcgctatgg aaatcgcgat gaaaggaatc gaagccaagg cgtgcggggt cgcgcgctat
      601 tttcctttcg tctacgttta ctacgaggag ggcgggatca agaacttcgg catgctcgga
      661 cacgaagcaa cgccgctgcg gtcgatggcg gcgtatgttc agtcgatcag ggccttgagc
      721 agtttggagt atcttggcga tatcgagtcg aaaccggatg gaataaaggt gggccgcttc
      781 tttggccgcc gcgatgaacg tgctccgcat gtgctggttc tttacaccgg acaaatcgat
      841 ggctcgaccg tggtaaagct gccgtttcgt gcggcgcgtg ccgaggggat ggatggacga
      901 gtgttgccgc tcgaaagcga tggctcgatg atgataccgg acggactggc ctatctgtgg
      961 attgatcggg caaccgccga aacgctgatc aaaacagaca catcggtcgc gcgactgcac
     1021 ctactcggaa agcagtcgca agtcgccccg gcggagcgat ccaaggtaat attgcgctac
     1081 gagcaggagc acacgccttc gcagcggtcg gcgcagcgct acttgctgaa gaaggaagta
     1141 gcgcggaatc tgccgatcag cgttaggatc gagaacctcg acaaggctga ccggcggatc
     1201 accgcgcagc tccgtttgcc aggagtcggc gaagccgcga cttgggaaac aaaaaccatc
     1261 gatgtgccag cgcacggctt cgcgcggctg gcgtggcgcg gtgatgcatc gaaccacctc
     1321 gatacggtca aagtgagctt catcacgatc tcggcgacat cggaccgcag cgttccgatc
     1381 agccccctcg tcctcccctt tgtcgtcgaa gggtcgctcg aagcgtattt ggctggtcac
     1441 gcgaagaaag agcgcctgcc agctgcggac ctcgcacgct ggcgcaccaa cgtggcgccg
     1501 aacggaaaaa tgaaactctc agcctcgccg gaggcgtggc ggatggacgt cgcgttcgac
     1561 ggcggaggct cctgggcgtt cccgcaattt gagctgccgt ctcactgcga cctcgagact
     1621 gcaacaggca ttgtcctgcg agcgcgcatt ctgcatccgg ctcggcacgt caccttctac
     1681 gctcagataa acgaacgtga gcgcttccaa gtcgctgacg ttttccccgc ggacggcgag
     1741 tggcactcgg tcttcattcc gttttcagag cttgtgccct cgcctggtca cgtggacatg
     1801 cagaacgctc ggctcgaccc gaaacggatc gcgcaactct cgctcggctt cgtggcgaac
     1861 ggtctcagcg gtgcgctcga gatca
//
LOCUS       DASPLT010000191         1550 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_24323, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000191 DASPLT010000000
VERSION     DASPLT010000191.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1550)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1550)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1550
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_24323"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..591
                     /locus_tag="VEA63_02015"
     CDS             <1..591
                     /locus_tag="VEA63_02015"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HYD82786.1"
                     /translation="RVSLSATGFYKTPEIFWDWAKAKGRPFAYFAGGAAVSEVEVDGY
                     TGMTRVRRVDIVHDVGDSLNPGIDRGQIEGGFVQGMGWLTSEELKWDASGRLLTHSAS
                     TYQIPAIGDAPMEFNVTLLPHHAAAAAVHGSKAVGEPPLMLAISVREAIRDAVASVGG
                     AAGAREGPVELASPATGEAVWLAMERLRVARGCGRP"
     gene            945..>1550
                     /locus_tag="VEA63_02020"
     CDS             945..>1550
                     /locus_tag="VEA63_02020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD82787.1"
                     /translation="MIVAFRQAVRKFAVGVGLGWLATMAGANEFLLRESGEPGPFLEG
                     TQDAAFIEEIQRRAVLYFVEQTDAKTGLTRDRAPADGGASGIPGSIAASGFALTGWCI
                     ADARGWLPPGEAVERARTTLRFFWEHVEHERGWFYHFVHMETGRRAWKSEASTIDTAL
                     FLKGAIAAREYFDDPEVRELVNALYARIDWQWALNGGTTLTH"
BASE COUNT          267 a          355 c          641 g          287 t
ORIGIN      
        1 cgggtgagct tgtcggcgac gggattttat aagacgccgg aaattttctg ggattgggcg
       61 aaggcgaaag ggcggccgtt tgcgtatttc gcgggtggcg cggcggtgtc ggaggtggag
      121 gtggatggct acacggggat gacgcgcgtg cggcgggtgg acatcgtgca cgacgtgggc
      181 gattcgctga atccgggaat cgatcgcgga cagattgaag gcgggttcgt gcaggggatg
      241 gggtggctga cgagcgaaga gttgaagtgg gatgcgagcg ggcggctgct gacgcacagc
      301 gcgagcacgt atcagattcc ggcgatcggg gacgcgccga tggagtttaa cgtcacgctg
      361 cttccgcacc acgcggcagc ggcggcggtc catgggagca aggcggtggg cgagccgccg
      421 ttgatgctgg cgatttcggt gcgcgaggcg attcgcgatg cggtggcgag cgtgggtggt
      481 gcggccggcg cgagggaagg gcccgtggag ttggcgtcac cggcgacggg ggaggcggtg
      541 tggctggcga tggagcggct gcgcgtggcg aggggatgcg ggcggccgtg acgagtggcc
      601 aatttagttt aaccacgaat ggacacgaac ggacacgaat tcagaggagc cgaaggaggc
      661 tttgcgggag agtccggtga tcgggggtag gagcctgctg gcaggcgatg agtgtgccgg
      721 ggtgaacgac gcgttcatat cgcctgcaaa caggctccta cccgttgcat ggttatggca
      781 cgccgcgtgg gacgcgtggt cctaattctt tcccgggcgg cgaaaagttt gtgaatgttc
      841 gggatgcggg ggctctcggg agattttacg atggaaaatc accgatgcgc cgattggtaa
      901 gacgtgcggt tcgcgtgagg cgcgccggaa ttttggactt accgatgatc gtagcgtttc
      961 gccaagccgt caggaaattt gccgtgggag tgggattggg ttggttggcg acgatggcgg
     1021 gagcgaacga gtttctattg cgcgaatcgg gtgagccggg cccgtttctc gaggggacgc
     1081 aggacgcggc gttcatcgag gaaattcagc ggcgcgcggt gctttatttt gtcgagcaga
     1141 cggacgcgaa gacagggctt acgcgggacc gggcgccggc ggacggcggt gcgagcggaa
     1201 ttccggggag catcgcggcg tcgggtttcg cgctgacggg ctggtgcatc gcggatgcgc
     1261 gcggttggct gccgccgggc gaggcggtgg agcgggcgag gacgacgctg cggtttttct
     1321 gggagcacgt ggagcacgag cgcggctggt tttatcattt cgtgcacatg gagacgggcc
     1381 ggcgcgcgtg gaagagcgag gcgtcgacga tcgacacggc gttgttttta aaaggcgcga
     1441 tcgcggcgcg ggaatatttc gacgatccgg aagtgcgaga gctggtgaac gcgttgtatg
     1501 cgcgaatcga ttggcaatgg gcgctgaacg gcgggacgac gctcacgcat
//
LOCUS       DASPLT010000192         1588 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_248283, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000192 DASPLT010000000
VERSION     DASPLT010000192.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1588)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1588)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1588
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_248283"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1516
                     /locus_tag="VEA63_02025"
     CDS             <1..1516
                     /locus_tag="VEA63_02025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD82788.1"
                     /translation="KPLWAAFLDMYRTKKFIVLAMELLLAVLFVGVAMALPLPGFFKV
                     TIALLWVAAFASSTQDICGDGIYLTALDKKAQANAAGVQSMFWNLGRVVATGVLISGM
                     EALATQEGWSATKMWMSIWITAGVGMALLAAYHVWALPTGSVSHRPENARQVIGDFLG
                     TATTFFHKRAFWGMIAFVFLYRFGEGLLLVEGKLFLQSTVESGGLGLSAGQVANIDAV
                     WGTGAMIVGGIVGGMFLGKIGLKRALPILGLCLNVPHFTFVYLSQAGAAGHGLDYATI
                     ATLVSIEKFGYGFGFIGNMVYMMQQIAPGRCTMTHYAFATALMNFMLVPTTMISGPLA
                     EWLGFSTFFVVVMFASVPSVIAAWKAPSPLEGDDAKAAAGAEPNQERVTVDDPTRLSA
                     ADRAVQLLAGRASIYAMLNVLVILLIDAKILGSLQGRDAGAGATQFYLLVASAALKLF
                     LAWRTYRFANEAAEEGARTGEKIYVRNARGAKIATWVCAVATVIVLTFAGRLAF"
BASE COUNT          245 a          351 c          630 g          362 t
ORIGIN      
        1 gaagccgttg tgggcggcgt tcctcgacat gtatcgcacg aagaaattca tcgtgctggc
       61 gatggagttg ttgctggcgg tgttattcgt gggcgtggcg atggcgctgc cgctgccggg
      121 ttttttcaag gtcacgatcg cgttgctgtg ggtggcggcg tttgcgtcgt cgacccagga
      181 tatttgcggc gatggaattt atctgacggc gctcgacaag aaggcgcagg cgaacgcggc
      241 gggcgtgcag agcatgtttt ggaatttggg gcgcgtggtg gccacgggcg tgttgatcag
      301 cgggatggaa gcgctggcga ctcaagaagg ctggtcggcg acgaagatgt ggatgtcgat
      361 ctggatcacg gcgggcgtgg gaatggcgtt gctcgcggct tatcatgtgt gggcgttgcc
      421 aacgggaagc gtgtcgcacc gcccggagaa cgcgcggcag gtgatcggcg atttcctggg
      481 gacggcgacg acgttttttc acaagcgcgc gttttgggga atgatcgcgt tcgtgttttt
      541 atatcggttc ggcgaggggt tgttgctggt ggaaggaaag ttgtttctgc aatcgaccgt
      601 ggagagcggc gggttggggt tgagcgcggg gcaggtggcg aacatcgatg cggtgtgggg
      661 aaccggggcg atgattgtgg gcggaatcgt gggcgggatg tttctcggaa agatcggttt
      721 gaaacgggcg ctgccgattc tggggctgtg tttgaacgtg ccgcatttca cgttcgttta
      781 tttgagccag gcgggtgcgg cggggcacgg gctggattat gcgacgatcg cgacgttggt
      841 gagcatcgag aagttcggct acggcttcgg gttcatcggc aacatggttt acatgatgca
      901 gcagatcgcg ccgggccggt gcacgatgac gcactacgcg tttgcgacgg cgctgatgaa
      961 tttcatgctg gtgccgacga cgatgatttc gggaccgctg gcggagtggc tcgggttttc
     1021 gacgttcttc gtcgtggtga tgtttgcgtc ggtgccgtcg gtgatcgcgg cgtggaaggc
     1081 gccctctccg ctcgaaggtg atgatgcgaa ggcggcagcg ggcgcggagc cgaatcagga
     1141 gcgcgtgacc gtggacgatc cgacgcggtt gtcggcggcg gatcgcgcgg tgcagttgct
     1201 ggccgggcgg gcgtcgattt acgcgatgtt gaacgtgctg gtgatcctgc tgatcgacgc
     1261 gaagattttg ggttcgctgc aagggcgaga tgcgggcgcg ggcgcgacgc agttttattt
     1321 gttggtggcg agcgcggcgc tgaaattgtt tttggcgtgg cgcacgtatc gattcgctaa
     1381 tgaggcggcg gaggaaggtg cgcggacggg cgaaaaaatt tatgtgcgca acgcgcgtgg
     1441 cgcgaagatc gcaacgtggg tgtgcgcggt tgcgacggtg atcgtgctga cgttcgcggg
     1501 gcggctggcg ttttgagcgg cgaaccgcgg atgacgcgga ggtcgctcct ccatttttag
     1561 tcgtctgtgg gcggtgcgtc ggatccgg
//
LOCUS       DASPLT010000193         1722 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_24338, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000193 DASPLT010000000
VERSION     DASPLT010000193.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1722)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1722)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1722
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_24338"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1682)
                     /locus_tag="VEA63_02030"
     CDS             complement(<1..1682)
                     /locus_tag="VEA63_02030"
                     /inference="COORDINATES: protein motif:HMM:NF027900.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5060 domain-containing protein"
                     /protein_id="HYD82789.1"
                     /translation="MTLTFKGPHASETGSPNPFRNFRLNVTFKHSSGRTFVVPGYFAA
                     DGDAHDSSATSGDKWRVHFSPDATGTWTYKVSFRTGADVAISTSATAGAATSFDGESG
                     SFTVDGTNKSGADFRAKGRLKEVGQHYLQHAGTKEYFVKAGAGSPENFLAYADFDNTS
                     KGKGLLHRYAAHAKDYRSGDPTWKGGKGKAIIGALNYLAAKKMNSLYFLTMNVGGDGN
                     DVFPWTTKSERYRYDVSKLAQWEIVFEHMDKRGIHLHLVTQETENDQLLDGGSLGVQR
                     KLYYRELVARFSHHLGVTWNLGEENTNSASQRSAFADYINGLDPYMSLIAVHTYPQDR
                     DKVYTPMLGDDVIGAASLQLQSPSIVHTETSKWVKKSAAAGRKWVVAVDELGGASVGA
                     KPDANDSSRTEIRHRVLWGSLLAGAAGLEWYFGYDYAHDDLTCEDWRSRDKLWTQTHY
                     AAQFIRDYMPLPLVTNHNAITSSTADYVLGKEGVAYAVYLPAGATTNITLPKSETYTV
                     RWFNPRAGGNLRTGTVASVKGGSTVSLGRPPAETSLDWVALLRRSSTSGGSSG"
BASE COUNT          292 a          579 c          492 g          359 t
ORIGIN      
        1 cccgacgaac cgccggacgt gctgctgcgg cgcagcaacg cgacccagtc gagcgacgtc
       61 tccgcgggcg gacgccccag cgacaccgtc gagccgcctt tcaccgacgc caccgttccg
      121 gtgcgaagat tgccgcccgc gcggggatta aaccaccgca ccgtgtaagt ctcgcttttc
      181 ggcagcgtga tgttggtcgt cgcgcccgcc ggcagatata ccgcgtaagc cacgccctct
      241 ttccccagca cgtaatccgc cgtcgatgac gtgatcgcat tgtgattcgt caccagcggc
      301 agcggcatgt agtcccggat gaactgcgcc gcgtaatgcg tctgcgtcca gagtttgtcg
      361 cggctgcgcc aatcctcgca cgtgagatcg tcgtgcgcgt agtcgtatcc gaaataccac
      421 tccagccccg ccgctccggc gagcaggctg ccccacagca cgcggtgccg gatctccgtg
      481 cgcgaactgt cgttcgcatc gggtttcgcg ccgacggacg ctcctccgag ctcatcgacc
      541 gccaccaccc actttcttcc cgccgccgcg gattttttca cccacttcga cgtctccgtg
      601 tgcacgatcg acgggctctg caactgcagc gaagccgcgc cgatcacatc gtcaccgagc
      661 atcggcgtgt aaaccttgtc tcgatcttgt ggatacgtgt gcaccgcgat caggctcata
      721 tacggatcga ggccgttgat gtagtcggca aacgcgctgc gctgcgacgc ggagttcgtg
      781 ttctcctcgc cgagattcca ggtgacgccg aggtgatggc tgaaccgcgc cacgagctcg
      841 cggtagtaga gcttgcgctg cacgcccagc gagccgccgt cgagcagttg atcgttctcc
      901 gtttcttgcg tcaccaggtg cagatggatg cctcgcttgt ccatgtgctc gaaaacgatt
      961 tcccattgcg cgagcttgct cacatcgtag cggtagcgct cggatttggt cgtccatgga
     1021 aacacatcgt tgccgtcgcc accgacgttc atcgtcagaa agtagagcga gttcattttc
     1081 ttcgccgcga ggtagttgag cgcgccgatg atcgccttgc ccttcccgcc cttccacgtc
     1141 gggtcgccgc tgcggtaatc cttcgcgtgc gccgcataac gatggagcag tcctttgcct
     1201 ttcgacgtgt tgtcgaaatc cgcgtaagcc agaaaattct cgggactgcc cgcgccggct
     1261 ttgacgaaat attccttcgt gccggcatgc tgcaggtagt gctggcccac ttccttcaac
     1321 cgccctttcg cgcggaaatc cgcgccggat ttgttcgtcc cgtcgaccgt gaagctgccg
     1381 gattccccat cgaaactcgt ggccgccccc gccgttgccg acgtggaaat cgccacatcc
     1441 gcgcccgtgc gaaacgacac tttgtaagtc caggtgcccg tcgcatccgg cgaaaagtgc
     1501 acgcgccatt tgtcgcctga agtcgcgctg ctgtcgtgcg catcgccgtc cgccgcaaaa
     1561 tagcccggca ccacgaacgt gcgccccgac gaatgcttga acgtcacatt gagccgaaag
     1621 tttcggaatg gattcggcga acccgtctcg ctcgcgtgag ggcctttgaa ggtcagcgtg
     1681 attttatgcc attgtcgctg ctcgccggaa agagtcgtcc tg
//
LOCUS       DASPLT010000194         2529 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_146128, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000194 DASPLT010000000
VERSION     DASPLT010000194.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 2529)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2529)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2529
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_146128"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..465
                     /locus_tag="VEA63_02035"
     CDS             <1..465
                     /locus_tag="VEA63_02035"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HYD82790.1"
                     /translation="RRGRILNTASIAGFQPGPLLAVYHASKAFILSWSEALATELKDG
                     NTGVTLTTLCPGPTDTDFFPKADMVDTKVFQKGSVMGPQEVAEAAYIALMKGERIIVP
                     GGMNKAMVVGGRMMPISAQAKMNEKMYEDADLDERKREPGDVEAKEAASRRE"
     gene            685..1218
                     /locus_tag="VEA63_02040"
     CDS             685..1218
                     /locus_tag="VEA63_02040"
                     /inference="COORDINATES: protein motif:HMM:NF017970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1003 domain-containing protein"
                     /protein_id="HYD82791.1"
                     /translation="MSEHRGQTESTAPLREIAPVVERNIAALIARRAEEEKRRPAHDR
                     MADAIARFTGSIPFVYLHLAVFGGWIVINLGWTPLPAFDETFVVLAMIASVEAIFLST
                     FVLITQNRQAKLADKRADLDLQISLLSEHEITRLIALLAAVGKKLGVAEADHPDIDPL
                     TKDVAPEKVLERLDEDT"
     gene            complement(1261..>2529)
                     /locus_tag="VEA63_02045"
     CDS             complement(1261..>2529)
                     /locus_tag="VEA63_02045"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sorbosone dehydrogenase family protein"
                     /protein_id="HYD82792.1"
                     /translation="VSLAALLACSPATVFAAELRTGAEAMSDWSTDAPGVRRKITVDD
                     LPTPYATESARNNARAVARPDGARPQAPAGFRVNEFASDLKKPRLITTAPNGDIFVAE
                     SDANRIRVLRDTNGDGAADVNEVFTTDVRQPFGIAFYPLGDEPTFVYVANTGSVVRFP
                     YRADDLKANGAPEMIVENVSAGGRLTGGGHWTRDIVFSQDGQTLFLSVGSLSNVDDDQ
                     DEARRARIFAYNPSGGDERVYAWGIRNPVGLAIDPATGALWTSVNERDGLGDDLPPDY
                     ITRVKEGGFYGWPWYYLGANQDPRHQGKRPDVASKVVVPDVLIQAHSASLDLVFYTGE
                     QFPSRYRGNIFAAEHGSWNREKRTGYKVIMVPVRDGAAAGEYEDFLTGFVTPEGNVWG
                     RPVGVTVANDGALLVSDDGSNMIWRVAHQN"
BASE COUNT          503 a          724 c          867 g          435 t
ORIGIN      
        1 cgcaggggac ggattttgaa cacagcgtcg atcgcgggct tccagcccgg gccgctgctg
       61 gcggtttatc acgcgagcaa ggcgtttatt ctctcgtggt ccgaggcgtt ggccaccgag
      121 ttgaaggatg gcaacacggg cgtgacgctg acgacgcttt gtcctgggcc cacggatacg
      181 gatttcttcc cgaaggccga catggtcgac acgaaggttt ttcaaaaagg cagcgtgatg
      241 ggaccgcagg aagtcgccga ggcggcctac atcgcgttga tgaaagggga gcggatcatc
      301 gtgcccggcg gcatgaacaa ggcgatggtc gtgggcggcc ggatgatgcc catttcggcg
      361 caggctaaga tgaacgagaa gatgtatgaa gatgccgacc tcgatgagcg gaagcgtgag
      421 ccgggcgacg tagaggcgaa agaagcggcg tcgcggcgcg aatgaacaat ccaccccaac
      481 caacgcattt atcttttgcc ttatgaaaac ccagaccacg tcaaagaacg aaacgaaaaa
      541 agcggcgggc cgcgcggaaa agcccgcgac cggagcgaat atggaaaaat cgtcaaaggt
      601 gcagaaggat gcggaggcga ggagtcgcgg cgaatcgccg gtggcgaaca agctaagtga
      661 gcgctcgccc cgtcaggaga acctttgagc gagcaccgcg gacagacgga gagcacggcg
      721 ccgctgcgcg agatcgcgcc ggtggtggag cgaaacatcg cggcgctgat tgcgcgccgg
      781 gctgaggagg aaaaacgtcg gcccgcgcac gatcggatgg ccgatgcgat cgcgcgtttc
      841 accggcagta ttccgttcgt ctacctgcac ctcgcggtgt tcggtgggtg gatcgtgatc
      901 aatctcgggt ggacgccact accggcattc gacgagacgt tcgtcgtgct cgcgatgatc
      961 gcatccgtgg aggcgatctt tttgtcgacg ttcgtgctga tcacgcaaaa ccgtcaggcc
     1021 aagctcgcgg ataaacgcgc cgacctcgat ctccagatca gtttgctgtc ggagcatgag
     1081 atcacgcgtc tgattgcact gctggcggcg gtcggaaaaa agctcggcgt ggcggaggcg
     1141 gaccatccgg acatcgaccc gctcaccaaa gacgtggcgc cggaaaaggt cttggagcgg
     1201 ctggacgaag atacgtgacg cgcacgttaa cgtgccgtcg aaacaggttt gccggggcca
     1261 tcagttctgg tgcgctacgc gccagatcat gttggagccg tcgtcgctca cgaggagcgc
     1321 gccgtcgttt gcgaccgtca cgccgacagg acgaccccag acgttgcctt caggcgtgac
     1381 gaagcccgtg aggaaatcct cgtattcgcc ggcggcggcg ccgtcgcgca cgggcaccat
     1441 gatcactttg taaccggtgc gcttttcgcg attccacgag ccgtgctcgg cggcgaaaat
     1501 gtttccacga tagcgcgacg ggaattgttc gccggtgtag aagacgaggt cgagcgaggc
     1561 cgaatgcgcc tggatcagca cgtccggcac gacgactttc gacgcgacgt cgggacgttt
     1621 gccttggtgg cgcgggtcct gattcgcgcc gagataatac caaggccagc cgtagaagcc
     1681 gccttctttc acgcgcgtga tgtagtccgg tggaagatcg tcgccgaggc cgtcgcgctc
     1741 gttgacggag gtccaaagcg cgcccgttgc aggatcgatc gcgagtccga cgggattgcg
     1801 gatgccccag gcgtaaacgc gctcatcgcc gccgctcgga ttgtaggcga agatgcgcgc
     1861 gcgccgcgcc tcatcctgat catcgtcgac attggaaagt gagccaacgg agaggaacag
     1921 cgtttggccg tcctgtgaaa aaacgatgtc gcgcgtccaa tgcccgccgc ccgtcaggcg
     1981 tccgccggcg gaaacgtttt cgacgatcat ctcgggggca ccgttcgcct tcaggtcgtc
     2041 cgcgcgatag ggaaagcgca cgacggaacc ggtgttcgcc acgtagacaa acgtcggctc
     2101 gtcaccgagc ggatagaagg cgatgccaaa gggctggcgc acgtccgtgg tgaacacctc
     2161 gttcacgtcg gcggcgccgt cgccgttggt gtcgcggagc acgcgaatgc ggttggcatc
     2221 gctttcggcg acgaagatgt cgccgttcgg cgcggtggtg atgaggcgcg gtttcttcaa
     2281 atcggaggcg aactcgttga cgcgaaaacc cgcgggagct tgcggtcgcg cgccatccgg
     2341 gcgtgcgaca gcgcgcgcgt tattgcgcgc ggattccgtg gcgtagggcg tgggaagatc
     2401 gtcgacggtg atttttcgcc gcacgccagg agcgtcggtg ctccagtcgc tcatcgcttc
     2461 ggcgcccgtg cgcagctcgg cggcgaagac ggtcgcggga gaacaggcga gcagggcagc
     2521 caggctaac
//
LOCUS       DASPLT010000195         1462 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_104381, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000195 DASPLT010000000
VERSION     DASPLT010000195.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1462)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1462)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1462
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_104381"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..250)
                     /locus_tag="VEA63_02050"
     CDS             complement(<1..250)
                     /locus_tag="VEA63_02050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD82793.1"
                     /translation="MSGFIAAKMTNNEARMTNYFSSALSVTLCFNFRFTLSPSSSRTA
                     FVHRHRRAASPNLHTHRKLRPWHDACNMQGETCSNDRYA"
     gene            237..611
                     /locus_tag="VEA63_02055"
     CDS             237..611
                     /locus_tag="VEA63_02055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009514451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009143 - nucleoside triphosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HYD82794.1"
                     /translation="MNPLTDSTTTVAELKARVLAFARERDWEQFHAPKNLSMALAAEA
                     AELMEHFLWTTSEASRAAVMEPAKRAKIAEELADIVIYSLEFANVSGLDVAAAIEAKM
                     AANAKKYPVEKARGRADKYTEL"
     gene            complement(619..861)
                     /locus_tag="VEA63_02060"
     CDS             complement(619..861)
                     /locus_tag="VEA63_02060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HYD82795.1"
                     /translation="MPTYVYETIPQIPGVEPRRFEMRQSMNDLALKTDPVTGEPVRRV
                     ISGGFGFVAQKMSGSSSSGAAAPSTAACGPRCACCR"
     gene            complement(984..1328)
                     /locus_tag="VEA63_02065"
     CDS             complement(984..1328)
                     /locus_tag="VEA63_02065"
                     /inference="COORDINATES: protein motif:HMM:NF013361.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylpyruvate tautomerase MIF-related protein"
                     /protein_id="HYD82796.1"
                     /translation="MPYLKIQTNLPLTEKSEQTVLKSASSLVARELGKPESFVMVALQ
                     ADTPMLFAGTDAPVAFLELKSIGLDAGKTKILSQALCRLIEDHLGIASDRVYVKFIDV
                     NHGMWGWKGDVF"
BASE COUNT          302 a          375 c          519 g          266 t
ORIGIN      
        1 ttgcgtaacg gtcattggaa caggtttcac cctgcatatt gcaggcgtcg tgccaagggc
       61 gaagttttct gtgcgtgtga agattgggtg acgctgcgcg acgatgacgg tgcacgaagg
      121 cggtgcgtga ggatgacggc gaaagggtga agcggaagtt gaagcacagc gtgacggaaa
      181 gcgcggagga gaaatagttc gtcattcgcg cttcgttatt cgtcattttt gccgcgatga
      241 atccgctcac cgattcgaca acaacggtcg ccgagctgaa ggcgcgggtg ctggcgttcg
      301 cgcgcgaacg cgactgggaa cagtttcacg cgccgaagaa cctgagcatg gctctcgctg
      361 cggaagcggc ggagctgatg gagcactttt tgtggacgac ttcggaggca tcgcgcgcgg
      421 cggtgatgga gcccgcgaag cgggcgaaga tcgccgagga acttgcggat atcgtgatct
      481 actcgctcga gttcgcgaac gtgagcggcc tcgatgtcgc cgcggcgatc gaagcgaaga
      541 tggcggcgaa cgcgaaaaag tatcccgtcg aaaaagcgcg cggtcgcgcg gacaagtata
      601 cggagcttta gcgagagctc agcgacagca cgcgcaacgc gggccgcagg cggcggtcga
      661 aggagcggcg gcgccggagg aggagctgcc ggacattttc tgcgcgacga agccgaagcc
      721 gccactgatg acgcgacgca cgggctcgcc ggtgacgggg tcggttttga gcgcaaggtc
      781 gttcatcgat tgacgcattt cgaaacgccg cggttcgaca ccggggatct gcgggatggt
      841 ttcgtaaacg taggtcggca tggcgtcggg aataaacgcg aggcgtgaaa aggatcaagc
      901 tgcggcgttc atgcagggaa gttggaaggc tgcagcgagg aaaatgcggg gcgcttgtcg
      961 gggcataaag cccctctcgc agtctagaac acgtcgcctt tccagcccca catgccatgg
     1021 ttcacgtcga tgaatttcac gtagacgcga tcggatgcga tgccgaggtg atcctcgatg
     1081 agccggcaga gtgcctggct gaggattttg gttttcccgg catcgagccc gatgcttttc
     1141 aactcgagaa acgcgacggg cgcatcggtc ccggcgaaga gcatcggcgt gtccgcctgg
     1201 agggcgacca tgacgaacga ttcgggtttg ccgagctctc tggcgaccag gctagaagcg
     1261 cttttgagca cggtctgctc ggatttctcc gtgagcggga gattggtctg aatcttcagg
     1321 taaggcatgg ctgcgcgggg ttgggggtta gcacgccgga ccagcctgaa gcatcgaata
     1381 actcgttcgc ggccgcaatc gcgacgcgca gaaagcgcgc aggcgagcgg catgtgcgat
     1441 ggaaaattgc ggcgcgcgcg at
//
LOCUS       DASPLT010000196         1670 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_207826, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000196 DASPLT010000000
VERSION     DASPLT010000196.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1670)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1670)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1670
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_207826"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..271)
                     /locus_tag="VEA63_02070"
     CDS             complement(<1..271)
                     /locus_tag="VEA63_02070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012372831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA N-terminal domain-containing protein"
                     /protein_id="HYD82797.1"
                     /translation="MTSLSLSPSLWETVKCDFKELFPEDVFQMWFEPMVCLEATEDSM
                     ALGVPNDFAAIWIHDNYLDLISQRLRLTAGRLVNVSLKKTDASRAP"
     gene            complement(782..>1670)
                     /locus_tag="VEA63_02075"
     CDS             complement(782..>1670)
                     /locus_tag="VEA63_02075"
                     /EC_number="1.17.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012377418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HYD82798.1"
                     /translation="ALAQLPPLATGFYVLELPAALSVAELAASSTHLPAGALLLRAFT
                     SDDAGDLQAFANLAREHRLGLAVEIAPPAILALANTLRSLGDLPLVFTLSSPLASLDS
                     THAIGRYRQLAEALRTIGSHAPIWIRNTAATAVQGNNSFLSKLLEASFLTGSLLCDGL
                     GDLVSIETENDPVRATKLTYNVLQGAGARISKTEFVACPSCGRTLFDLQTTTQRIRAQ
                     TGHLKGVKLAIMGCIVNGPGEMADADFGYVGGAPGKINLYVGKQCVQYNIPQNEADAR
                     LIALIKEHGKWVDPEPAHA"
BASE COUNT          347 a          419 c          591 g          313 t
ORIGIN      
        1 aaggcgcgcg cgaggcgtcg gtctttttca gcgagacgtt aacgagacgg ccggcggtga
       61 gtcggaggcg ctgcgaaatg aggtcgaggt agttgtcgtg aatccagatc gcggcgaagt
      121 cattcggcac gccgagcgcc atcgagtcct cggtggcttc gaggcacacc atgggctcga
      181 accacatctg gaagacgtcc tcagggaaga gttccttgaa gtcgcacttg acggtttccc
      241 agaggctggg agacagggac aaagaggtca ttggcagacg aaaagacagg tttattcaca
      301 ttgcgtcggg agcaggtcac tctcacgcct catttcgcct tctcctgtct gcctgccggt
      361 aacaaataga tgcccgacca tcgaaatgtg gggaaggggt gctaagtaaa ggtaaaatgt
      421 tgatggccaa gtcgtagagc agaccgatca ccggggcgag agaaacgcgt aaatgggggt
      481 gtagaaaaga acagctggac gcttcggaca acggacgagg gcgcagtaac tgtgaagcaa
      541 aaaaccattt caagacgaac ttcctaccaa gttgttcaca ttggtcctga gcataacttt
      601 tccgcagttt tttgttgcgc cagcggccgg aaaccagcgc gcggaaagca tcgggaattt
      661 cccgtaattg gataggaact acgcggccgg gcagacggct tcgcaagcgg gttggcgggt
      721 tacggaccgg tgacgaagcg gtgacggcga gtttaatttt gaatacacgc gcgggcgtcg
      781 gtcacgcatg cgcgggctcc ggatcgaccc attttccgtg ctctttgatc aaggcgatga
      841 ggcgcgcgtc ggcttcgttt tgcgggatgt tgtattggac gcactgcttg ccgacgtaga
      901 ggttgatctt gccgggagcg ccgccgacgt aaccgaaatc ggcgtcggcc atttcgccgg
      961 ggccgttcac gatgcagccc atgatggcga gcttcacgcc cttgagatga ccggtctggg
     1021 cgcggatgcg ttgcgtcgtc gtctgcaaat cgaagagcgt gcggccgcag ctcgggcagg
     1081 cgacgaactc ggttttagag atgcgtgcgc cggcgccttg gagaacgttg taggtgagct
     1141 tcgttgcgcg gacgggatcg ttttccgttt cgatgctgac gagatcgccg aggccgtcgc
     1201 agaggagcga gccggtgaga aaactcgctt cgaggagttt cgacaggaag gagttattcc
     1261 cctgcacggc ggtcgcggcg gtgttgcgaa tccagatcgg agcgtgcgag ccgatcgtgc
     1321 ggagcgcttc ggcgagttgg cggtagcggc cgatggcgtg agtcgagtcg agagatgcga
     1381 gcggagagct gagcgtgaaa accagcggaa ggtcgccgag agaacggagt gtgttcgcga
     1441 gtgcgaggat cgcaggcggt gcgatttcca ccgcgaggcc gagtcgatgt tctcgagcaa
     1501 gattcgcgaa ggcctgcaga tcaccggcgt cgtcggacgt aaacgcccgc agcagaagcg
     1561 cgccggctgg caggtgggtg gacgacgcgg cgagctcggc gacggacagt gcggcgggca
     1621 gttcgagcac gtagaaaccg gtcgcgagcg gcgggagttg ggcgagcgcg
//
LOCUS       DASPLT010000197         1739 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_104397, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000197 DASPLT010000000
VERSION     DASPLT010000197.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1739)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1739)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1739
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_104397"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..309)
                     /locus_tag="VEA63_02080"
     CDS             complement(<1..309)
                     /locus_tag="VEA63_02080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD82799.1"
                     /translation="MSQIDELLIEAKRVCGERWHKAPEYPWEVAAQFGAEVERLREAN
                     SKLAAAHHSMHEECQRLRAENAELRKAGKGLCSNCGFACSIVDETEELKARLAWFEGR
                     E"
     gene            complement(309..503)
                     /locus_tag="VEA63_02085"
     CDS             complement(309..503)
                     /locus_tag="VEA63_02085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD82800.1"
                     /translation="MLDDYDDDRSRVCSKAADLLGFGPDNNMTGLFEAYTTLVKAGLE
                     ATPQAVADQLERMSRSGWRV"
     gene            complement(536..745)
                     /locus_tag="VEA63_02090"
     CDS             complement(536..745)
                     /locus_tag="VEA63_02090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD82801.1"
                     /translation="MTVDEAIVFARSIRARIAHSDEREAMRVLADEVEALRQVAANHK
                     RALENAEHWRAQAEVWRARALEAEG"
     gene            complement(1001..1327)
                     /locus_tag="VEA63_02095"
     CDS             complement(1001..1327)
                     /locus_tag="VEA63_02095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD82802.1"
                     /translation="MAFGGVMLFLWFCVAVLRWIWRALFTRPALVAEQSEIDAARKLL
                     ETPRPVVGGSEWMRYRCTPEMCEAPVMRTELQIHDTQAPGHDYRTCTVCQERPRYSGA
                     SYRSAN"
BASE COUNT          273 a          625 c          506 g          335 t
ORIGIN      
        1 ctcccgcccc tcgaaccacg ccagcctggc cttgagctcc tcggtctcgt cgacgatcga
       61 gcaggcgaag ccgcagttcg agcacagccc cttgccggcc ttgcgcagct ccgcgttctc
      121 cgcgcgcagg cgctgacact cttcgtgcat ggagtggtgc gcagcggcca gcttgctgtt
      181 cgcctcgcgc aggcgctcca cctcggctcc gaactgtgcg gccacctccc aaggatactc
      241 aggcgccttg tgccatcgct cgccacacac gcgcttggct tcgatcagta gctcgtcgat
      301 ctgactcatc acaccctcca ccctgagcgc gacatgcgct cgagctgatc ggccacggcc
      361 tgtggcgtcg cttcgagccc ggccttgacg agcgtggtgt acgcctcgaa cagcccggtc
      421 atgttgttgt caggcccgaa ccccagtagg tcggccgcct ttgaacacac gcggctgcgg
      481 tcgtcgtcgt agtcgtcgag catagaccct ccaccctaag agaaagcccg gcggttcatc
      541 cctcggcctc tagcgctctc gctctccaca cctcggcctg agcgcgccag tgctcggcgt
      601 tttccagtgc gcgcttgtga ttggccgcca cctgcctgag cgcctccacc tcgtcggcca
      661 agacgcgcat ggcctcgcgc tcgtccgaat gtgcgatgcg cgctctgatg cttctggcaa
      721 acactatggc ttcgtcaaca gtcatggtgc cctcacttag agaaagcccc gagggaccat
      781 tcccccgggg ctcacgccat ctcacgaacg ggctagaagc ccggcgacta tacctcagcg
      841 cccgcgaaag tgtcgcagcg cgcggcgtag ctcttcgtcc tctgcaccgt cagcgaatcg
      901 gccgagcctg cgctcgagct tctccgctct gtctctgagc gcctcgttct cgcgctcgtc
      961 cttggtcttg gtcttcccga cccatcccat ggttgtcctc ctagttcgcg ctgcgatagc
     1021 tcgcgcccga gtagcgcggt cgctcttggc agaccgtgca cgtcctgtag tcgtgccccg
     1081 gcgcttgcgt gtcgtggatc tggagctcgg tccgcatgac cggcgcctcg cacatctcgg
     1141 gcgtgcagcg gtaccgcatc cactccgacc cgccgacaac gggccgcggc gtttcgagca
     1201 gcttgcgagc cgcatcaatc tctgactgct cggccacgag cgccggtcgc gtaaacagcg
     1261 cgcgccaaat ccacctgagc accgcgacgc aaaaccacaa gaaaagcatc acgccgccga
     1321 acgccatcag caactctatt ttgtctcccg aatcacgcat acaaacgttc ctgattggtt
     1381 gttgtgaaag atgcgtacgc cgccaacagc tgcagagagc agggtcccta ctgcgttggc
     1441 gacgtgtgtg aggtagagcg gggcggccta ccccctacta gacagaccgc cctcccccgc
     1501 tatcctcaac gctccgaggg agcgctttgg cggacgcagg agcgttccgc agccctcagc
     1561 ggcggtaagc ccagcggttg agtcgctctt gttcgagcag tgagagcggc tcgtgtcggc
     1621 cgcacagagt cgccttgtcg gttgggtatc tgcagcgccg tacgtacgga ccgcgcgccc
     1681 agagtcgagt tgctccgagc acagcgtcta cgcttgcggc ggtgcctcgg gcggcgctt
//
LOCUS       DASPLT010000198         2163 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_63679, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000198 DASPLT010000000
VERSION     DASPLT010000198.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 2163)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2163)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2163
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_63679"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..704
                     /locus_tag="VEA63_02100"
     CDS             <1..704
                     /locus_tag="VEA63_02100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glutamate--tRNA ligase family protein"
                     /protein_id="HYD82803.1"
                     /translation="TSMLEDLRWLGLAWAEGPDVGGPHAPYNQSERRAHYRAALAQLH
                     AARLIFPCTHSRRDVLEAATAPHADDEPVYPPAFRPPADAPLPLLGETISVNWRFRVP
                     DGETISFTDARFGEQHAIAGRDFGDFLVWRKDDVPSYQLACAVDDAAMQITEVVRGAD
                     LIKSTFRQLLLLRALGHTAPTYYHCPLMTDEKGERLAKRHDALSLRTLRERGTSPDAI
                     VQTFSQETAKSAEKH"
     gene            749..1276
                     /gene="mog"
                     /locus_tag="VEA63_02105"
     CDS             749..1276
                     /gene="mog"
                     /locus_tag="VEA63_02105"
                     /EC_number="2.7.7.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001954004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin adenylyltransferase"
                     /protein_id="HYD82804.1"
                     /translation="MPIRIGVLNISDRASAGVYADEPGQACLALLREWLLTPFEPDYK
                     IIPDDRATIEAELRRMADDSHCALVVTTGGTGPAPRDVTPEATCAVIEKELPGFGELM
                     RTTSLRYVPTAILSRQTAGIRGHTLIVNLPGRPKAIRENLEAVFPAIPYCIDLIGGPR
                     LETNEAAMKAFRPKS"
     gene            1395..>2163
                     /locus_tag="VEA63_02110"
     CDS             1395..>2163
                     /locus_tag="VEA63_02110"
                     /inference="COORDINATES: protein motif:HMM:NF017770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF898 family protein"
                     /protein_id="HYD82805.1"
                     /translation="MPEPSLRAPADDPLLAAAFTFHGSTRDYFRIWSVNTFLTLITLG
                     VYSAWAKVRKRRYLRGNTELLGHRFDYTADPRRILIGNLIVVVLFLGYALFGAVYPPL
                     RLLALALGVIFLPWIIVRSLAFNAHNTVWRGLRFRFHSSLSAAVMTYLLKPLLIPLSL
                     GLYYPAWARDAAEFRISRHRFGTAYFRFNTSAAPFYRAYLGAAGIVFIGGFFGGLLQM
                     HARDHAPAGTHVLPSILLSATALFVAKHYVFAELFNRM"
BASE COUNT          362 a          874 c          511 g          416 t
ORIGIN      
        1 ttacctccat gctcgaagac ctccgctggc tcggtctcgc gtgggccgaa ggtcccgatg
       61 tcggcggccc tcacgcgccc tacaaccaaa gcgagcgccg cgcccactat cgcgccgcgc
      121 tcgcccagct ccacgccgca cgcctgatct ttccctgcac gcactcgcgt cgcgacgtcc
      181 tcgaagccgc caccgcgccg cacgccgacg acgagcccgt ttacccgccc gctttccgcc
      241 cgcccgcgga cgccccactt ccgctgctcg gcgaaaccat ctccgtcaat tggcgctttc
      301 gcgtgcccga cggcgaaacg atttcgttca ccgacgcgcg cttcggcgaa caacacgcca
      361 tcgccggccg cgacttcggc gacttccttg tgtggcgcaa agacgatgtc cccagctacc
      421 aactcgcctg cgccgtcgac gacgccgcga tgcagatcac cgaagtcgtc cgcggcgccg
      481 acctgatcaa atccaccttc cgccaactcc tcctcctgcg cgccctcggc cacaccgccc
      541 ccacatatta tcactgtccg ctgatgaccg acgaaaaagg cgaacgcctc gccaaacgcc
      601 acgacgccct cagcctccgc accctccgcg aacgcggcac ctcgcccgac gccatcgtcc
      661 aaaccttttc acaggagacc gcaaagagcg cagagaaaca ttgaaccccc gttctccgct
      721 ccctccgctt cctcctgtaa aaacaaccat gcccattcgc atcggcgtcc tcaacatctc
      781 cgaccgcgcc agcgccggcg tctacgccga cgaacccggc caggcctgcc tcgccctcct
      841 gcgcgaatgg ctgctcacgc ccttcgagcc ggattacaaa atcattcccg acgaccgtgc
      901 gacgatcgaa gccgaactcc gccgcatggc cgacgattcc cactgcgccc tcgtcgtcac
      961 caccggcggc accggcccag cgccgcgaga cgttacgccc gaagccacat gcgctgtaat
     1021 tgaaaaagaa ctacccggct tcggcgaact catgcgcacc acgtcgctcc gctacgtgcc
     1081 caccgcaatt ctctcccgcc agaccgccgg cattcgcggc cacaccttga tcgtgaatct
     1141 gcccggccgc cccaaagcca ttcgcgaaaa cctcgaagct gttttccctg ccatcccgta
     1201 ttgcatcgat ctcatcggcg gcccgcgtct cgaaaccaac gaggccgcca tgaaagcctt
     1261 ccgcccaaaa tcctgacgca acggggggag gggcttcacg ccccgacggt tcgtcccgcg
     1321 ccggaaaacc gtttgcaatt ccttcatccc accaccctgc tcggccgcac tccatggatc
     1381 ttccgcatcc atccgtgccc gagccatcgc ttcgcgctcc cgctgacgat cctctgctcg
     1441 ccgctgcatt caccttccac ggctccaccc gcgactactt ccgtatttgg agcgtaaata
     1501 cctttctcac gctgatcacc ctcggcgtct attctgcgtg ggccaaggtc cgcaaacgcc
     1561 gctatcttcg cggcaacacc gaactgctcg gccaccgctt tgattacacc gccgacccgc
     1621 gccgcattct catcggtaac ttgatcgtgg tcgtgctgtt cctcggctac gcgctcttcg
     1681 gcgcggttta tccgccgctt cgactgctcg cgctcgctct cggcgtgatc ttcctgccgt
     1741 ggatcatcgt ccgctcgctc gccttcaacg ctcacaatac cgtctggcga ggccttcgct
     1801 tccgtttcca ttcgagcctg agcgccgccg tcatgactta cctgctcaag cccctgctga
     1861 ttcccctttc cctcggcctt tactatcccg catgggcgcg cgacgccgcg gagttccgca
     1921 tcagccgcca tcgcttcggc accgcgtatt tccgtttcaa caccagcgcc gcaccattct
     1981 accgcgctta tctcggcgct gctggaatcg ttttcattgg cgggtttttc ggtggcttgc
     2041 tgcaaatgca cgcccgcgat cacgcccccg ccggcacgca cgtcctcccg tcgatccttc
     2101 tctccgccac cgctctgttt gtcgcgaaac actacgtgtt cgccgagctt ttcaatcgca
     2161 tgt
//
LOCUS       DASPLT010000199         1025 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_290004, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000199 DASPLT010000000
VERSION     DASPLT010000199.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1025)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1025)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1025
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_290004"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1025)
                     /gene="malQ"
                     /locus_tag="VEA63_02115"
     CDS             complement(<1..>1025)
                     /gene="malQ"
                     /locus_tag="VEA63_02115"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HYD82806.1"
                     /translation="RFAESGRDQLEGCGSLVAFREHHAAWLEPFADFMALKTHFDGAP
                     WPAWPAAVREWSPELRAVLPPEVARDAERHIFYQYVFFAQWNRLRRYAAERGVGIIGD
                     VPIFVALDSVDAWLNRRVFRLSDQGDPEAVAGVPPDYYSSHGQLWGNPLYDWDHLAST
                     GYAWWIDRLRAAFELYDVIRLDHFRGFDTYWEISADALDARNGRWSKGPGLAFFQALQ
                     TALPAARFIAEDLGYIGPDVVQLRRAAGLPGMKVLQFAYGHDANNVNLPHNFPPDSVV
                     YTGTHDNLTTRGWLEQLPPAHENSVDEYFQLAGSRSAWPIIRTAFASVARLAIIPMQD
                     LLDLPAS"
BASE COUNT          207 a          295 c          352 g          171 t
ORIGIN      
        1 gctggcaggc agatcgagca ggtcctgcat cgggataatc gcgagccgcg cgacggaagc
       61 gaacgccgtg cgaatgatcg gccacgcaga ccgcgaaccg gcgagttgga aatattcgtc
      121 gaccgagttt tcgtgcgccg gcggaagctg ttcgagccag ccgcgcgtgg tgaggttgtc
      181 gtgcgtgccg gtgtaaacga cgctgtcggg cggaaaattg tgaggaaggt tcacgttgtt
      241 tgcgtcgtga ccgtaggcga actgaagcac cttcatcccc ggcaagccgg cggcgcggcg
      301 gagttgaacg acgtccggac cgatgtagcc gagatcttcg gcgatgaaac gcgcggcggg
      361 cagcgcggtt tgcagcgctt gaaaaaacgc gagtccaggg cctttgctcc accggccgtt
      421 gcgagcgtcg agcgcgtccg cggagatttc ccaatacgtg tcgaagccgc gaaagtgatc
      481 gaggcggatg acatcgtaga gttcgaacgc cgcgcggagc cgatcgatcc accacgcgta
      541 accggtgctc gcaagatgat cccaatcgta gagcggattg ccccagagtt gtccgtgcga
      601 cgaatagtaa tccggcggaa caccggcgac ggcttccgga tcgccttggt cgctgagtcg
      661 gaaaacgcgc cgattcagcc acgcgtccac actgtcgagc gcgacgaaaa tcggaacgtc
      721 gccgataatg ccgacgccgc gttcggcggc gtagcggcgc agtcgattcc attgcgcgaa
      781 gaaaacgtat tggtagaaaa tgtgtcgctc ggcgtcgcgc gcgacttccg gcggaagcac
      841 ggcgcgaagt tccgggctcc attcgcgaac ggccgctggc cacgcaggcc agggcgcgcc
      901 gtcgaaatgc gttttgagcg ccatgaagtc ggcgaacggc tcgagccagg cggcgtgatg
      961 ttcgcgaaac gcgacgagcg agccgcagcc ttcgagttga tcgcgtcctg attcggcaaa
     1021 gcgat
//
LOCUS       DASPLT010000200         1324 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_125139, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000200 DASPLT010000000
VERSION     DASPLT010000200.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1324)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1324)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1324
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_125139"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..605
                     /gene="radC"
                     /locus_tag="VEA63_02120"
     CDS             <1..605
                     /gene="radC"
                     /locus_tag="VEA63_02120"
                     /inference="COORDINATES: protein motif:HMM:NF000642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HYD82807.1"
                     /translation="SLSDAELLAIFLRVGTRGRSAIDLGRDLIARFGSLSALLNAEPR
                     AAVPGVGSAKWAQLKAAAELGRRALAETARRTDALSSPTAVRDYLRLLLAEREHEVFV
                     AVFLDAQNRVIASEELFRGTLTQTSVYPREVVKAALRHNAAGVILAHNHPSGVAEPSQ
                     SDQMLTETLKRALTMIDVRVLDHFIVGRGAACSFAERGLL"
     gene            681..914
                     /gene="rpmB"
                     /locus_tag="VEA63_02125"
     CDS             681..914
                     /gene="rpmB"
                     /locus_tag="VEA63_02125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004631999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HYD82808.1"
                     /translation="MARVCQVTGKKPMVGHNVSHANNKTKRRFLPNLQYRRFWVEAEN
                     RWVRLRVSNAGLRLIDKNGIESVLADLRAKGEI"
     gene            929..1084
                     /gene="rpmG"
                     /locus_tag="VEA63_02130"
     CDS             929..1084
                     /gene="rpmG"
                     /locus_tag="VEA63_02130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010364212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HYD82809.1"
                     /translation="MREKIKLESTAGTGHFYTTTKNKRTTPDKIEIKKYDPKARKHVI
                     YKETKLK"
BASE COUNT          252 a          440 c          396 g          236 t
ORIGIN      
        1 cctccttgtc ggatgcagag ttgctggcga tctttcttcg cgtcggcaca cgcgggcgca
       61 gtgccattga cctgggccgc gacctcatcg cgcgcttcgg ctcgctgagc gcgttgctca
      121 acgccgagcc gagggctgca gtgccgggcg tgggttctgc gaaatgggcg caattaaagg
      181 cggccgccga attgggccgg cgcgcactgg ccgaaacggc acgacgtacc gacgccctga
      241 gttctcccac cgcagttcgc gactacttgc gcctgctgct cgccgagcgc gagcacgaag
      301 tcttcgtcgc cgtctttctc gacgcgcaga accgcgtcat cgccagcgaa gaactgttcc
      361 gcggcacgct gacccagacg agcgtctacc ctcgtgaggt ggtcaaggcc gccctccgtc
      421 acaacgcggc aggcgtcatc ctcgcccaca atcatccgtc cggcgtggcg gaaccaagcc
      481 agtccgatca gatgcttacc gagaccctga agcgcgccct gacgatgatc gacgtgcggg
      541 tgctggacca tttcatcgtc ggtcgcggcg cggcctgctc cttcgccgaa cgcggcctgc
      601 tgtaggaggc attccggctt gcccgtgtcc cgcttttacc tctataatcc gcggcttttg
      661 cgatttccgg agacgctgtc atggcgcgag tttgccaagt caccggcaag aagccgatgg
      721 tcggacacaa tgtgtcccac gccaacaaca agaccaagcg tcgcttcctg cccaatctgc
      781 agtaccgccg cttctgggtc gaagccgaga accgctgggt gcgtctgcgc gtgtccaacg
      841 ccggcctgcg tctgatcgac aagaacggca tcgagtccgt gctggccgat ctgcgcgcca
      901 agggcgaaat ctaaacggag cctgaatcat gcgcgaaaag atcaagctcg aatcgacggc
      961 cggcaccggc cacttctaca ccacgaccaa gaacaagcgc acgacgcccg acaagatcga
     1021 aatcaagaaa tacgatccga aggcgcgcaa gcatgtgatc tacaaagaga cgaagctgaa
     1081 gtaaccggct tcccgctcga cgaagaaccc gcctcccggc gggttttttg ttttccgcgc
     1141 tcgcaaatga aaaagcccgg gaggtcccgg gctttttact gggcgccgtg cgcgcttatg
     1201 cgtagctgcg cagcctcagc gagaagtcgc gcaacggcgc gatgccgctc gcctctgcac
     1261 ggtggcacca gtcctgcagt tgcttgagga gttgctcccg cgacgccgtg gagcgctccc
     1321 agac
//
LOCUS       DASPLT010000201         1106 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_146210, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000201 DASPLT010000000
VERSION     DASPLT010000201.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1106)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1106)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1106
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_146210"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..464
                     /gene="def"
                     /locus_tag="VEA63_02135"
     CDS             <1..464
                     /gene="def"
                     /locus_tag="VEA63_02135"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012372926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HYD82810.1"
                     /translation="AAGGIGLAAQQIGRALQLCVVDLRRAEVDFTWEIDGAKTPLDLF
                     MPMTIVNPQITIAPGTDEYYAEEGCLSFPDIRGDVARPDAITVKYQDVGGVPHLLRCD
                     GLFARCILHEFDHLNGVLFIDRMEKKVRAKIDADVKALAKATRDAAKPKTA"
     gene            547..>1106
                     /locus_tag="VEA63_02140"
     CDS             547..>1106
                     /locus_tag="VEA63_02140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007362731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HYD82811.1"
                     /translation="MTQKSDGMNYAPQGAPRPVVKAGEFPFAAAFLEHGHIYGQCNGL
                     IEAGAELRWVYDADPKKVEAFREKYPHVRVARSYDEILADPEIKLVTAAAIPHERGAI
                     GCRTMAAGKDYFTDKTPFTTLEQLDEARRVVASTGRKYLVYFSERLHVESAIHAGDLV
                     HGGAIGRVVQVIGLGPHRLNKASRPA"
BASE COUNT          214 a          409 c          303 g          180 t
ORIGIN      
        1 acgccgccgg cggcatcggc ctcgccgcgc aacaaatcgg gcgcgccctt caactctgcg
       61 tcgtcgatct ccgccgcgcc gaggtggatt tcacctggga aatcgatggg gctaaaaccc
      121 cgctcgatct cttcatgccg atgacgatcg tgaacccgca gatcaccatc gcgcccggca
      181 ccgacgaata ctacgccgag gaaggctgcc tgtcgtttcc cgacattcgc ggcgatgtgg
      241 cgcgccccga tgcgatcacc gtgaaatacc aggacgtcgg cggcgttccg cacctcctac
      301 gctgcgacgg cctgttcgca cgctgcatcc ttcacgaatt cgatcacctc aacggcgtgc
      361 tcttcatcga tcgcatggag aaaaaagttc gcgccaagat cgacgccgac gtgaaagcgc
      421 tcgcgaaagc cacccgcgac gccgcgaaac cgaagaccgc ctagcgcgcc gcgcagcaac
      481 gctcgtcctt tgtcgtcaac cgccgcgtca ctccacccgg cgcgaccccg cttctcgctt
      541 ctttccatga ctcaaaaatc cgacggcatg aactacgcgc cgcaaggcgc gccccgcccg
      601 gtcgtgaaag ccggcgagtt tccgttcgcc gctgcgttcc tcgaacacgg ccacatctac
      661 ggccagtgca acggcctgat cgaagccggc gccgagctgc gctgggtcta tgacgcagat
      721 cccaaaaagg tggaggcgtt tcgcgagaaa tatccccacg tccgcgtcgc ccgctcttac
      781 gacgagatcc tcgccgatcc cgaaatcaag ctggtgaccg cggccgcaat tccccacgaa
      841 cgcggcgcga tcggctgccg cacgatggcc gcgggcaaag actacttcac cgacaagacg
      901 ccgttcacca ccctcgagca gctcgacgaa gcccgccgcg tcgtcgcctc caccggtcgc
      961 aaatacctcg tctatttcag cgagcgcctg cacgtcgaat ccgcgattca cgccggcgat
     1021 ctcgtccatg gcggcgccat cgggcgcgtt gtccaagtga tcggactcgg accgcatcgg
     1081 ctcaacaaag cgtcacgccc cgcctg
//
LOCUS       DASPLT010000202         1063 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_84490, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000202 DASPLT010000000
VERSION     DASPLT010000202.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1063)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1063)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1063
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_84490"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..292)
                     /locus_tag="VEA63_02145"
     CDS             complement(<1..292)
                     /locus_tag="VEA63_02145"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HYD82812.1"
                     /translation="MKLLHLEDNARDADLVRELLTDEWPTCEITLVDTREAYTAAIEH
                     RGYDVILSDYALPHFNGSEALQIAREKAPEVPFIFLSGNIGEDLAIEAVRAGA"
     gene            complement(407..856)
                     /locus_tag="VEA63_02150"
     CDS             complement(407..856)
                     /locus_tag="VEA63_02150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HYD82813.1"
                     /translation="MPHLRPILLAEDNPNDAELVLSALHGMKISNQIVVVGDGEQALD
                     YLYSRGAYAGSKRPTPAVVLLDLKMPRVDGREVLRQLREDPALSMTPVVVFTSSKEEI
                     DVLQSYRLGANAFVVKPVDFQDFIIAVTKLGSFWATLNEPPPSIVPT"
BASE COUNT          205 a          265 c          391 g          202 t
ORIGIN      
        1 gcgcgccggc ccgcacggct tcgatggcga gatcctcgcc gatattgccc gacaggaaaa
       61 tgaacggaac ctctggcgct ttctcgcgcg cgatttggag cgcttcactg ccgttgaaat
      121 gcggcagggc gtagtcggac aggatgacgt cgtagccgcg gtgttcgatg gctgcggtgt
      181 aggcttctcg tgtgtcgacc agcgtgatct cgcaggtcgg ccattcatcc gtgagcagct
      241 cgcgcacgag atcggcgtcg cgggcattat cctcgaggtg gaggagtttc attggagcta
      301 gaaactggcg tttgtgaaaa aatgtatcat gcggcgtgaa acgaaccgca gcgtcgaacg
      361 cgtcgcgggt gtgaaaagca aggggagggg taggtgaacc gcgtcgtcag gtcgggacga
      421 tcgagggcgg cggctcgtta agagtagccc agaaagagcc gagcttcgtc acagcgatga
      481 tgaaatcctg gaaatctact ggcttcacga caaaggcgtt tgcgccgagc cggtagctct
      541 gcagcacatc gatttcttct ttggaggacg tgaagacgac gacgggcgtc atggatagcg
      601 cgggatcttc ccggagctgg cgaaggacct cgcgaccgtc aacgcggggc attttcaaat
      661 cgagcagcac gacggcgggt gttgggcgtt tcgatccagc gtaggctccg cgggagtaga
      721 gataatcgag cgcctgttcg ccgtcgccta cgacgacgat ttgattggag attttcatgc
      781 cgtggagggc ggagaggacg agttccgcgt cgttgggatt atcctcggcg aggagaatgg
      841 ggcgaaggtg gggcatggcg gtctgcgcgc gaagggagaa aacggtgttt cggatccggg
      901 cctaggcaaa tggcgccgtg gttccggaac ggggcgccgg gtcgttggga agggagaaaa
      961 agaaggtggc gccggcgccg gcgctgcttt ccgcccaggt gcggccgccg tgtcgggtga
     1021 tgatccggcg gacgttggcg aggccgatgc cggtgccttc gaa
//
LOCUS       DASPLT010000203         1171 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_146279, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000203 DASPLT010000000
VERSION     DASPLT010000203.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1171)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1171)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1171
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_146279"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..649
                     /locus_tag="VEA63_02155"
     CDS             <1..649
                     /locus_tag="VEA63_02155"
                     /inference="COORDINATES: protein motif:HMM:NF012904.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HYD82814.1"
                     /translation="ALAEHAQKIGADAIATIAPSFFKPATVEELVAWCAMVAAAAPAL
                     PFYYYHMPAMTGVGITATEFLRHADGRIPTLAGVKFTHEDLNDYRAARAYADGKYAIL
                     FGRDEILLSGLELGAPGAVGSTYNYAAPLYTRIINAHRAGDAELARAEQAKARAFIDV
                     MVAKGGLPAGKAIMSLIGVDCGPVRLPLRRLSPVDIASLREALSTVGFFDFASRV"
     gene            732..>1171
                     /locus_tag="VEA63_02160"
     CDS             732..>1171
                     /locus_tag="VEA63_02160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD82815.1"
                     /translation="MNVSAAGQAAIEWTQAPALPDAIGFAGSFAGVSGDALLVGGGAN
                     FPDRPPWQGGTKVWHDRVFAFDADNNRWLDAGRLPQPNGYGVSVTVPDGLVLIGGGDA
                     VKNFADAWLARYDADTRRVSFTRWPNLPVPLAMAAGALVEKTIYV"
BASE COUNT          196 a          378 c          366 g          231 t
ORIGIN      
        1 cgcgctggct gagcatgccc aaaaaatcgg agcggatgcg atcgccacga ttgcccccag
       61 ctttttcaag cccgcaaccg ttgaagagct cgtcgcctgg tgcgcgatgg ttgccgccgc
      121 cgcacccgcg ttgcccttct actattacca catgcccgcg atgaccggcg tcgggatcac
      181 cgcgacggag ttcttgcgcc acgcggacgg ccggattccc acgctcgccg gcgtgaagtt
      241 cacgcacgaa gatttgaatg actaccgcgc cgctcgcgcg tatgcagatg gcaaatacgc
      301 gatcctgttt ggccgcgacg aaatcttgct gtcgggtctc gaactcggcg cgccgggggc
      361 ggtgggcagc acttacaact atgcggcgcc gctctacacc cgcattatca atgctcaccg
      421 cgccggcgac gcggagctcg ctcgcgcgga gcaggcgaaa gcccgcgcgt ttatcgacgt
      481 gatggtcgcc aaaggcggcc ttcccgccgg caaggcgatc atgagcctca tcggcgtcga
      541 ttgcggtccg gtccgattgc ccctgcgccg gctttcgccg gtggatatcg catcgttgcg
      601 cgaggccttg tccaccgtcg gcttcttcga ttttgcaagc cgggtttgat cgaggacgct
      661 gccaacgtct cgctcgttcc gcagccatga aacgtttttt taaaatctct ttcccagccc
      721 tcctctttgc aatgaacgtg agcgccgccg gccaagctgc catcgagtgg acccaagcgc
      781 ctgcgctgcc cgatgcgatt ggcttcgcgg gaagtttcgc cggtgtcagt ggcgacgcgc
      841 tgctggtcgg aggcggcgcg aattttcctg accgaccgcc gtggcaaggc ggaacgaagg
      901 tgtggcacga tcgcgtcttc gcgttcgacg cggataacaa ccgctggctc gacgccggcc
      961 ggctgccaca accgaacggc tacggcgtca gcgtcaccgt gccagacggg ctggtgctga
     1021 tcggcggagg cgatgcggtg aagaacttcg ccgatgcctg gctcgcgcgc tacgacgccg
     1081 atactcgtcg ggtttcattc acgcgctggc caaatttgcc tgttccgctc gcgatggccg
     1141 caggagcgtt ggtggaaaaa acgatttatg t
//
LOCUS       DASPLT010000204         2136 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_166581, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000204 DASPLT010000000
VERSION     DASPLT010000204.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 2136)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2136)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2136
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_166581"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..822
                     /locus_tag="VEA63_02165"
     CDS             <1..822
                     /locus_tag="VEA63_02165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD82816.1"
                     /translation="LPAQPDVIIRALNTHDAPTRFTRELDYDFVWRNKCLLFETRVGR
                     GSWIVSGLNFDSALRHGGPEGPWLLGRLLERAQSLPEPKNAIPIQTLRDAVAQSPFTT
                     GALVSGYHRTLKHVGEKSQGITYRENAGTSLRIRQEEPLHEITWETAPVPAAQRTTFV
                     FAGGTSFHRYAPGSLGFALRVNGRKVIDFDSTREPMTWRSADGDYTLTYVPALNQSSW
                     KETMGLFYLSVPASNVAAGAPNQLSVYSVGHDNVRWFALNPYADVLDGGCVPFTR"
     gene            819..>2136
                     /locus_tag="VEA63_02170"
     CDS             819..>2136
                     /locus_tag="VEA63_02170"
                     /inference="COORDINATES: protein motif:HMM:NF014116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate binding domain-containing protein"
                     /protein_id="HYD82817.1"
                     /translation="MTLPRLMPSNSSLLRLFRAVLLLCPLTPLLHGADPLMDRTQTEL
                     SRWQPRLPETTAKNAIPNSSFELASYGWSSLGEPAGWSTGLSSLYGTVTERESHEGRR
                     SLEIVLGPGKTPVASFDVWPAATTVQHSPIAMNLGWITIKPGETYTLSAYMKANRPGI
                     PARLVMRFGNDLSASPSPHNEEKTVTLTEKWERYSFTFKANDVSVCVGVGPDLRNQPD
                     AEATVWIDAVQFERSDRATAYTPHADLEVSLDSGRLGHLYSVGSPVTLRLAVSNRAAR
                     STEVNVSVSLTDYFDEPVPGFQHTVRVDAKGEIEESIPLPPPGPGYYRLHAKITAPGL
                     NDEIRFPLALIHEFAGSDALFGLNHPTPTIELMDQFRRAGVLWGRDWSCDWHQVEPQQ
                     DVFDWAESDRQIARLEQSGWKIMSMLPGFPSTKWSSSVPADHVVPPG"
BASE COUNT          383 a          791 c          566 g          396 t
ORIGIN      
        1 ctccccgcgc aacccgacgt gatcatccgc gcgctcaaca cccacgacgc acccacgcgt
       61 ttcactcgcg aactcgatta cgacttcgtc tggcgcaaca agtgcctgct tttcgaaacc
      121 cgcgtcggcc gtggctcgtg gatcgtcagc ggcctcaact tcgattccgc ccttcgccac
      181 ggcggccccg agggtccgtg gctgctcggc cggttgctcg agcgcgccca gtcgctgccc
      241 gaaccgaaaa acgcgatccc gatccaaacc ctccgcgacg ccgtcgcgca atcgccgttc
      301 accaccggcg cgctcgtctc cggttatcac cgcacgctga aacacgtcgg cgagaaatcg
      361 cagggcatca cctaccgcga aaacgccggc acctcgctcc gcatccgcca ggaggagccg
      421 ttgcacgaga tcacttggga aaccgcgccc gttcccgcgg cccagcgcac caccttcgtc
      481 ttcgccggcg gcacgtcttt ccatcgttat gcccccggct ccctcggctt tgccctccgg
      541 gtcaacggcc gaaaggtgat cgatttcgac tccacgcgcg aaccgatgac atggcgcagc
      601 gccgacggag attacacgct cacctacgtt ccagcgctca accagtcctc gtggaaggag
      661 accatggggc tgttttatct ttccgttccc gcttccaacg tggcagccgg cgctcccaac
      721 cagctcagcg tgtattcagt cggccatgac aatgtccgct ggttcgccct caacccctat
      781 gccgacgttc tcgacggcgg ctgtgttccc ttcacccgat gaccctgcca cgcctcatgc
      841 cttcgaactc gagcttgctc cgactttttc gcgccgtcct tctgctatgt ccattgacgc
      901 cgttgctgca cggcgccgat ccgttgatgg atcgcaccca gacagaactt tcccgctggc
      961 agccgcgttt gcccgaaacg accgcgaaga acgcgattcc caacagcagc ttcgagctcg
     1021 cctcctacgg ctggtcgagc ctcggcgaac ctgccggatg gagcaccggt ttatcctcgc
     1081 tttacggcac ggtcaccgaa cgcgaaagcc acgagggccg gcgcagcctc gagatcgtcc
     1141 tcggccccgg caaaactccc gtggcgtcct tcgatgtctg gcccgccgcc acgaccgtgc
     1201 aacattctcc gatcgcgatg aacctcggct ggatcacgat caagcccggg gaaacctaca
     1261 cactctccgc ctacatgaag gcgaaccgtc ccggcattcc cgcgcgtctt gtcatgcgct
     1321 tcggcaacga tctctcggcg tccccctcgc cgcacaacga ggagaaaacc gtcacgctca
     1381 ccgaaaaatg ggagcgctac agtttcacct ttaaagccaa tgacgtcagc gtctgcgtcg
     1441 gcgtcggccc cgacctgcgg aaccagcccg atgccgaagc caccgtctgg attgacgccg
     1501 tgcagttcga gcgctccgac cgcgccaccg cctacacgcc tcacgccgac ctcgaggtct
     1561 cactcgattc cggccggctc ggccatctct actccgtcgg ctcgcccgtt acactgagac
     1621 tcgccgtcag caatcgtgcc gcacgctcca cggaagtgaa cgtcagcgtc tcgttgaccg
     1681 attacttcga cgaacccgtt ccaggctttc aacacacggt gcgtgtcgat gcgaagggcg
     1741 agatcgagga gtcgatcccg ctcccgcctc ccggccccgg ctactatcgt ctccacgcaa
     1801 aaataacggc gccaggcttg aacgatgaaa tccgtttccc gctggcgctc attcacgaat
     1861 tcgccggctc cgatgccttg ttcggactca accatccgac acctacgatc gaactcatgg
     1921 atcagttccg ccgtgccggc gtgctctggg gacgagattg gtcgtgcgac tggcaccagg
     1981 tcgaaccgca gcaagacgtc ttcgactggg cggagtccga tcgccagatc gcccgcctcg
     2041 aacaatcggg ctggaagatc atgtcgatgc tgcccggctt tccctcgacg aaatggtcgt
     2101 ccagcgtgcc cgcggaccat gtcgttccgc ccggcg
//
LOCUS       DASPLT010000205         1214 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_24696, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000205 DASPLT010000000
VERSION     DASPLT010000205.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1214)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1214)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1214
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_24696"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1214)
                     /locus_tag="VEA63_02175"
     CDS             complement(<1..>1214)
                     /locus_tag="VEA63_02175"
                     /inference="COORDINATES: protein motif:HMM:NF012686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD82818.1"
                     /translation="GSKDYFAGGYRQTWWLLGASSFISNFSCWTFTGAAGIAYTYGFL
                     IFAVYLTDVLGFVVSYAWFAPRFRQLRMVTAMDALRLRFGRFNEQFFTWIGFIRSLGF
                     AAVWLIGLSIILSTAFNFPPVPVILVTGVVVIVIALVGGNWAVAASDFIQLLVLMSIT
                     IVVGVMTLIKIGGIGAFIDQIPETHFQVLRPAGSIPYDWLYVVTLLFSAVYDRNDIQR
                     AAKYIPAKDSRHARRSALVPLFGYMIMPIFWFIPPLAAFTLAPDLMEQKLMNNPSEAS
                     YIAVCLAVLPQGLIGLMIAAMFSATIASMDVALNKNAGFFVKNFYQPIVRKKAGDREL
                     LLAGEISTVVFGALIVALAMSVVVGSTISLFDAFLYLNAYLGLPLSIPMFLGMLIKKV
                     PRWAGWGTTLFG"
BASE COUNT          310 a          388 c          325 g          191 t
ORIGIN      
        1 cgccgaacaa cgtcgtgccc cagccggccc agcgcggcac cttcttgatg agcatgccga
       61 ggaacatcgg aatgctcaac ggcagcccga gataggcgtt gagataaaga aatgcgtcga
      121 acaacgaaat cgtcgagccc acgacgaccg acatcgcgag cgccacgatc agcgcgccga
      181 acacaacggt ggaaatctcg cccgcgagaa ggagctcacg atcgccggct ttcttgcgca
      241 cgatcggctg ataaaaattc ttcacgaaga agcccgcatt cttgtttagc gccacgtcca
      301 tgctcgcgat cgtcgccgag aacatcgcgg cgatcatcaa cccgatcagc ccttgcggca
      361 gcaccgccag gcacacggcg atgtaggacg cctcgctcgg attgttcatc aacttctgct
      421 ccatcaaatc cggcgccagc gtgaacgccg ccagcggtgg gatgaaccag aagatcggca
      481 tgatcatgta gccgaagagc ggcacgagcg cggagcgccg cgcatgccgc gagtctttcg
      541 ccggaatata tttcgccgcc cgctgaatgt cgtttcgatc gtagaccgcg ctgaagagca
      601 aggtgacgac gtaaagccag tcgtaaggaa tcgagccggc gggccgcagc acttgaaagt
      661 gcgtttccgg aatctgatcg atgaacgcac cgatgccgcc gattttgatc agcgtcatca
      721 ccccgaccac gattgtgata ctcatcaaca ccagcagttg aataaaatcg ctcgcggcca
      781 ccgcccagtt tccgccaacg agtgcgatca cgatcaccac cacgccggtg acgagaatta
      841 ccggcacggg cggaaagttg aacgccgtcg agaggatgat cgacagcccg atcaaccaca
      901 ccgcggcaaa accgagcgat cgaatgaaac caatccacgt gaaaaactgc tcgttgaaac
      961 gcccgaagcg cagccgcagc gcgtccatcg ccgtcaccat tcgcaactgc cgaaatcgcg
     1021 gcgcgaacca cgcgtagctg acgacgaaac ccagcacatc cgtcagatac accgcgaaaa
     1081 tcaggaagcc atacgtgtag gcaattcccg ctgcgcccgt gaacgtccaa caactgaagt
     1141 tagaaataaa cgaactcgcg ccgagcaacc accacgtctg ccgatatccg cccgcgaagt
     1201 agtctttgct gccg
//
LOCUS       DASPLT010000206         1031 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_146304, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000206 DASPLT010000000
VERSION     DASPLT010000206.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1031)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1031)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1031
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_146304"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..586)
                     /locus_tag="VEA63_02180"
     CDS             complement(<1..586)
                     /locus_tag="VEA63_02180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD82819.1"
                     /translation="MTSLNSGSLRPRIVRPQLSLLFFVSGAVALVYETVWQRQFALVF
                     GSAAPATAAVLAAYFAGLGAGSVAIGRWARRCERPLRAYALLECGIAIGALTTPLWLA
                     GAETLYPVIYDSAAMQPAVFVALRLVVAFAVMAMPTFCMGGTLPLLARWIDAGERRWG
                     RTAGWLYVVNTAGAALGALCFPFVLFPRLGLAQTL"
     gene            complement(757..>1031)
                     /locus_tag="VEA63_02185"
     CDS             complement(757..>1031)
                     /locus_tag="VEA63_02185"
                     /inference="COORDINATES: protein motif:HMM:NF024240.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HYD82820.1"
                     /translation="AGPYEIAFGALVPKRGEADNLLVPVCVSSSHIAFGSVRMEPVFM
                     ILGQSAATTAVLAIDSGVAVQDVDYAALRERLLADGQVLHHDAPQQ"
BASE COUNT          242 a          377 c          277 g          135 t
ORIGIN      
        1 acagcgtctg cgccaatccg agtcgcggaa acaaaacgaa cgggaaacac aacgcaccca
       61 acgccgcgcc cgccgtgttg acgacataca gccaccccgc ggtccttccc caacgtcgct
      121 cacctgcatc gatccagcga gccagcagcg ggagcgtgcc gcccatgcag aacgtcggca
      181 tcgccattac cgcgaaagca accaccagcc gaagagccac aaacactgca ggctgcatgg
      241 cggcgctgtc atagataacc ggatacagcg tctccgcgcc ggccagccag agcggcgtcg
      301 ttagcgcgcc gatcgcgatc ccgcactcca gcaacgcata cgctcggaga ggtcgttcgc
      361 aacggcgcgc ccagcgccca atcgccacac tgcccgctcc gagtcccgcg aaatacgcgg
      421 ccagcaccgc cgccgtcgcg ggggccgccg atccaaacac cagcgcaaac tgccgctgcc
      481 acaccgtctc gtaaaccagc gccaccgcgc ccgagacaaa gaacagcagc gaaagctgcg
      541 gacgaacgat ccgcggacgc aaagagccgg agttgagcga agtcacaaaa aaagacggca
      601 accgtcgccg tgttcggaac accctagact aaccgagaca aaccaaaacc aattcgttga
      661 gatcccggtt gtcgtctact tgtagcgatt ccccaccaga ttctaaacgc gtgagcgcgc
      721 tcgtcgtctg cagatccagc gcccgcatcg ctccgctcac tgctgcggcg cgtcgtgatg
      781 caagacctgc ccgtctgcca gcagtcgctc gcgcaaagcc gcgtaatcca cgtcctgcac
      841 cgccacgcca ctatcgattg cgagcacggc cgtcgtggcg gccgactgtc cgaggatcat
      901 gaatacaggt tccatgcgca ctgagccaaa cgcgatgtgc gaactcgaga cgcataccgg
      961 cacaagaaga ttgtccgcct cgccacgctt cggcaccagc gcgccaaacg cgatctcgta
     1021 aggcccagcc g
//
LOCUS       DASPLT010000207         2078 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_24706, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000207 DASPLT010000000
VERSION     DASPLT010000207.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 2078)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2078)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2078
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_24706"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1529
                     /locus_tag="VEA63_02190"
     CDS             <1..1529
                     /locus_tag="VEA63_02190"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HYD82821.1"
                     /translation="GDGWSWETAVNYSGAVQDFSNSNLVRTAAREEAVNSGELNLFAR
                     EQRAEVLERLFGSATGEFRSELISWDLKFVGADAVSLPGGGVDFAAGLETREESLEAD
                     SNVESRSATFAYDSGTTIDPFDERRRVSSVFAEMNLPLVGDANRRSGIHSADMTIAVR
                     HERYSDTDDPTVPKVSLRYQPTGDDWVLRFTYSRSFAAPALYELNAPTGTGFTNSLAE
                     FGSNQAQLQTLPVESLMPSRSRNFSGGVVWTPQAVAGLSVSVDYFAIEQTDVISNLNA
                     AGVIDQVFHDVEVNGVASPYASILHVGGYNGPTVTEPGQLSSLGIDNFYFVIPAASNL
                     GAQRLDGADVRLAYERVIGEARLRWDSGTTYYRRFDVQVAPGAPFTATAGLVTGLNGS
                     IPRWRSYNTLTWTWRAWSVNVAHTYYAAMRDATWTADFLPDYPERIPAHSVFDASVSY
                     EWKGGWRWLDRVQLTIGVNNMGDRLPPKSATFDGIANADVSEFDPIGRLYYVAAKHRF
                     "
     gene            1780..>2078
                     /locus_tag="VEA63_02195"
     CDS             1780..>2078
                     /locus_tag="VEA63_02195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HYD82822.1"
                     /translation="MRSCTIRRPAEELYPFWRSLENLVTVIKHPVSITRISDVESHWR
                     VSAPGHDRFVEWDAVIINDEPNELIAWRSKDGAEIENAGTVRFERAPGDEGTEVTV"
BASE COUNT          369 a          549 c          749 g          411 t
ORIGIN      
        1 tgggtgacgg gtggtcgtgg gagacggcgg tgaactacag cggggcggtg caggatttct
       61 cgaattcgaa tctcgtgcga accgcggcgc gcgaggaggc ggtgaactcg ggcgagctca
      121 atctttttgc acgcgagcag cgggcggaag tgttggagcg tttgttcggc tcggcgacgg
      181 gtgagttcag gagcgagctg atttcatggg acctgaaatt cgtcggcgcg gacgccgttt
      241 ctttgccggg cggcggggtc gattttgcgg cggggttgga gacgcgcgag gaatctctgg
      301 aggcggattc gaatgtggag agccggtcgg cgacgttcgc gtatgattcc ggaacgacga
      361 tcgatccttt cgacgaacgg cgtcgagtga gctcggtgtt cgccgaaatg aatctgccgc
      421 tggtgggcga cgccaaccgg cgcagtggta ttcattcggc ggatatgacg atcgcggtgc
      481 gacacgagcg ctacagcgac accgacgatc cgacggtgcc gaaggtctcg ttgcgctacc
      541 agccgacggg ggacgattgg gtgttgcgat tcacgtattc gcgatcgttc gcggcgccgg
      601 cgctgtatga gttgaatgcc ccgaccggga cggggttcac gaattcgctg gcggagttcg
      661 ggagcaatca ggcgcagttg caaacgctgc cggtggagtc gttgatgcca tcgcgctcgc
      721 gaaatttttc ggggggagtg gtgtggaccc cgcaggcggt ggcgggcttg tccgtgtcgg
      781 ttgattattt cgcgatcgag cagacggacg tgatcagcaa tctgaacgcg gccggtgtga
      841 tcgaccaggt gtttcacgac gtcgaggtga acggagtggc gtcgccgtat gcctcgatac
      901 tccatgtcgg cggttataac gggccaaccg tcactgagcc ggggcagctc tcgtcgctgg
      961 ggatcgacaa tttttacttt gtgattccgg cggcgagcaa tctcggtgcg cagcggttgg
     1021 acggagcgga cgtgaggctc gcttacgagc gggtgatcgg tgaggcgcgg ctgcgctggg
     1081 atagcgggac gacctattat cggcggttcg acgtgcaggt ggcgccgggc gctccgttca
     1141 cggctacggc cggcttggtg acagggttga acggaagcat tccgcgctgg cggagctaca
     1201 atacgctgac gtggacctgg cgcgcgtgga gcgtgaatgt cgcgcatacg tattatgcgg
     1261 cgatgcgcga cgcgacgtgg acagcggatt tcctgcccga ctatcctgag cgaattcccg
     1321 cgcattcggt gttcgatgcg agcgtaagct acgagtggaa aggcgggtgg cggtggcttg
     1381 atcgcgtgca gctgacgatt ggcgtgaaca acatggggga tcgtttgccg ccgaagtcgg
     1441 cgacgttcga tggcatcgcg aatgcggacg tgagcgagtt cgatcctatc ggacggcttt
     1501 actacgtcgc cgcgaagcat cgtttttgag gacgccatcg gggcgaagag agcggtcgag
     1561 gtgggtgaac ggcccggttt ttcgacgcat gtgcttcgcg cgaacggctt atgccgctac
     1621 ccggaggcgc ccccatggcg cggcggcgcg cttggaatat cgtgcggctt acccatggaa
     1681 acccaatctt ctgcggagcc cacaacggct cccaccccat caggaaccag aacagcccga
     1741 agcaaaatcg ccgttccggg caatcgcggc gtcaaggtcg tgcgttcgtg cacgattcgc
     1801 cgacctgccg aggagctcta tccgttttgg cgctcgctcg aaaaccttgt gaccgtgatc
     1861 aagcatcctg tttcgatcac ccgaatctcc gacgtcgaat cgcactggcg cgtgagcgct
     1921 ccggggcacg atcgcttcgt cgagtgggac gccgtgatca tcaacgacga accgaacgag
     1981 ttgatcgcgt ggcgttcgaa agatggcgcg gaaatcgaga acgcgggcac ggtgcgtttc
     2041 gagcgcgcgc cgggcgacga aggcacggaa gtgacggt
//
LOCUS       DASPLT010000208         1192 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_268753, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000208 DASPLT010000000
VERSION     DASPLT010000208.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1192)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1192)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1192
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_268753"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..835
                     /locus_tag="VEA63_02200"
     CDS             <1..835
                     /locus_tag="VEA63_02200"
                     /inference="COORDINATES: protein motif:HMM:NF019256.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1588 domain-containing protein"
                     /protein_id="HYD82823.1"
                     /translation="IDPDPEIFANYRPALLQAFQDEISLFVSDLIRENRSVLELMTSE
                     YTYLNEDLALHYGITDIKGDHFRKVKLRQPERYGLLGKGGVQMVTSYANRTTPVIRGA
                     YIMENFMGVPPANPPPNVEAFPETPEGATVAETVRERLERHRDIPACAGCHDVMDPLG
                     LALENFNAIGEFRLRDSDAGNVPIDASGKLADGTPLNGVNDLRAALVKRPDQFVQVFT
                     EKLMTYALGRGVEAHDMPAVRNIVRNAADDNYSFAAIVKGVIDSEQFQKMTVPADGMQ
                     Q"
     gene            958..>1192
                     /locus_tag="VEA63_02205"
     CDS             958..>1192
                     /locus_tag="VEA63_02205"
                     /inference="COORDINATES: protein motif:HMM:NF019222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1552 domain-containing protein"
                     /protein_id="HYD82824.1"
                     /translation="MLRSALATVSLPLLDAMIPASTALAQTAAVSPPRLGFFYFPHGA
                     VMQNWTPEKAGTDFELKSLLAPLEAYKSQITIVS"
BASE COUNT          259 a          375 c          357 g          201 t
ORIGIN      
        1 catcgatccc gatccggaaa ttttcgcgaa ctaccggccg gctttgctgc aggctttcca
       61 ggatgagatc agcctgttcg tgtccgacct gattcgcgag aaccgcagcg tgctcgagct
      121 gatgacgtcg gagtacacgt acctgaacga agacctcgca ctgcattacg gcatcaccga
      181 catcaagggc gatcacttcc gcaaggtgaa gctgcggcag ccggaacgtt acggcctgct
      241 cggcaagggc ggcgtgcaga tggtgacgtc ttatgcgaac cgcaccacgc cggtgattcg
      301 cggcgcctac atcatggaga acttcatggg cgtgccgccg gccaatccgc caccgaacgt
      361 cgaagcgttt ccggaaacgc cggaaggcgc gacggtggcc gaaaccgtgc gcgaacgtct
      421 cgaacggcat cgcgacatcc cggcctgcgc cggctgccat gacgtgatgg atccactcgg
      481 cctcgcactc gagaacttca atgcgatcgg cgaattccgt ttgcgcgaca gcgacgccgg
      541 caacgtgccg atcgacgcga gcggcaagct ggccgacgga acgcccctga acggcgtcaa
      601 cgatctacgc gcggcactcg tcaaacgccc ggaccagttc gtgcaggtgt tcaccgaaaa
      661 gttgatgacc tacgcgctcg gccgcggcgt cgaagctcac gacatgccgg cagtgcgcaa
      721 catcgtgcgc aatgcggccg acgacaatta cagtttcgcc gcgatcgtca aaggcgtgat
      781 cgacagtgaa cagttccaga aaatgaccgt gccggcggat ggtatgcagc aataaacacg
      841 acgtcatacc cgcgaaagcg ggtatccaga ctggattcct gcttccgcgg gaatgacgaa
      901 agacaatggg agaacgaagc catgttcaac acgaagaaac acctctcacg cagaacagtg
      961 ctccgcagcg cgctggcaac cgtgtcgctg ccactgctcg acgcgatgat tcccgctagc
     1021 accgcgctcg cgcagaccgc cgcggtgtcg ccgccgcggc tcggcttctt ctattttccg
     1081 cacggtgcgg tgatgcagaa ctggacgccg gagaaggccg gtaccgattt cgaattgaag
     1141 tcgctgctcg cgccgctcga agcgtacaag tcgcagatca cgatcgtgtc ga
//
LOCUS       DASPLT010000209         1478 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_290082, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000209 DASPLT010000000
VERSION     DASPLT010000209.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1478)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1478)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1478
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_290082"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..596)
                     /locus_tag="VEA63_02210"
     CDS             complement(<1..596)
                     /locus_tag="VEA63_02210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP/GTP-binding protein"
                     /protein_id="HYD82825.1"
                     /translation="MKTMKTSLVSLLVFATCGAAAAFGQSHSLEKKWESEATLKVPES
                     VLFDAERQVLYVTNIDGAPWGRDDKGSVGKLGLDGKVIEVDWVTGLQAPKGMALHEKH
                     LYVGDLGEVVVIDIEKGEIAARIAVEGAKGLNDVSIDKQGVVFVTDSEGKKLYRIENG
                     EPTVMLENLKGPNGVLAHNGAVYIVDGEALYRVMEDRS"
     gene            complement(860..>1478)
                     /locus_tag="VEA63_02215"
     CDS             complement(860..>1478)
                     /locus_tag="VEA63_02215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009508946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MTAP family purine nucleoside phosphorylase"
                     /protein_id="HYD82826.1"
                     /translation="PYGDVTYKTKGDFALINRHGYAFPLPPHSINYRANIRALADLGY
                     EDIVSLNSVGSLKKELPPGTFVSCSDYVCIQQGPMTFFDTELKGGAPGIANNLVPLLI
                     EKLAPEFTIHPGKVYVQFRGPRFETKAEIRIVQSWGDVVGMTAAHEADLCGEIGLRYN
                     SLALIDNYANGLEGTEIDFAKFKELVKDNQAKVNRLFSRMLEILA"
BASE COUNT          299 a          537 c          326 g          316 t
ORIGIN      
        1 aacgagcgat cctccatcac gcgataaagc gcctctccat cgacgatgta aaccgccccg
       61 ttgtgcgcca acacgccatt gggcccttta agattctcca gcatcaccgt cggttcgccg
      121 ttctcgatcc ggtaaagttt cttcccctcc gaatccgtca cgaacaccac gccttgcttg
      181 tcgatcgaca catcgttcaa gcccttcgcg ccttcgaccg cgatcctcgc cgcgatctcg
      241 cctttctcga tatcgatcac gaccacctct ccaagatcgc cgacgtaaag atgcttctcg
      301 tgcagcgcca ttcctttcgg cgcctgcaag cccgtcaccc aatcgacttc aatcaccttc
      361 ccgtcgagcc cgagcttgcc caccgagcct ttgtcgtcgc gaccccacgg cgcgccgtcg
      421 atgttcgtca cgtaaagcac ctgccgctcg gcgtcgaaga ggaccgattc cggcactttc
      481 aacgtcgcct cgctttccca ttttttctcg agtgagtgcg attggccaaa cgccgccgca
      541 gcgccgcacg ttgcgaatac caacagggaa acgagggatg tcttcatagt cttcatgggg
      601 tttgcgaata ccttacacag acgcacccgc gcgccttccg gttcgccgcg ccaactcttc
      661 ctcaaccgct ttcggcgtca taaaacgttc caccgaccag cccacgaatc gacccaaaga
      721 aaaccacaac gccacccgtt ccgccattcg tgtaaattcg ggtccattcg tggttcaaaa
      781 ctccgcccgt ccgccccttc atccatgtcc atccgcggtt aaaaatcctg ccgcccccct
      841 tattcctctc cgccgccgct cacgccagaa tctccagcat ccgcgagaac aaccggttca
      901 ccttcgcctg attgtctttc acgagttcct tgaatttcgc gaagtcgatc tccgtcccct
      961 cgagcccgtt cgcataattg tcgatcaacg ccagcgaatt atatcgcagt ccgatctcgc
     1021 cgcacaaatc cgcctcgtgc gccgccgtca tcccaacgac gtcgccccag ctttgcacga
     1081 tccggatctc cgccttcgtc tcaaaccgcg gcccgcgaaa ctgcacgtag actttcccag
     1141 gatgaatcgt gaactccggc gcaagtttct cgatcaacaa cggcacgaga ttattcgcga
     1201 tccccggcgc tccgcctttc agctccgtat cgaaaaacgt catcggcccc tgctgaatgc
     1261 agacatagtc gctgcacgac acaaacgtcc ccggcggcag ctccttcttc agcgagccga
     1321 ccgagttgag cgatacgatg tcctcgtagc cgagatccgc cagcgcacga atgttcgccc
     1381 gataattgat cgagtgcggc ggcagcggaa acgcgtaacc atgccggttg atcagcgcga
     1441 aatcgccctt cgtcttatac gtcacatctc cgtaaggc
//
LOCUS       DASPLT010000210         1792 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_166636, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000210 DASPLT010000000
VERSION     DASPLT010000210.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1792)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1792)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1792
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_166636"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..388
                     /locus_tag="VEA63_02220"
     CDS             <1..388
                     /locus_tag="VEA63_02220"
                     /inference="COORDINATES: protein motif:HMM:NF012936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="asparagine synthase-related protein"
                     /protein_id="HYD82827.1"
                     /translation="VEQRLPFLDHKLFETIKDIPASLLWRDGREKWVLREALKPFVSD
                     AVYAGKKQPFSAPRSALREDSALLALAQELLRSEAAGSVPFLDRAGLIALADRLMTLP
                     DQERSALESTVLMLMSVVVLQQRYRL"
     gene            complement(427..1203)
                     /gene="surE"
                     /locus_tag="VEA63_02225"
     CDS             complement(427..1203)
                     /gene="surE"
                     /locus_tag="VEA63_02225"
                     /EC_number="3.1.3.5"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0008254 - 3'-nucleotidase activity
                     [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HYD82828.1"
                     /translation="MHFLVTNDDGIDSRFLHELVAALRETGHRISVIAPKTEQSWIGA
                     AKSKLRPVHACVSERGLACDTWIVDGTPSDCVNIGLAHLLTTPVDGVISGINVGQNVS
                     LGFIIASGTIAGAWEGALHGLPAIAFSQDLSFEIFDRLKENGGVPDASLLATLKTSAR
                     HAARLAPELLAATPPRKWIVHNVNFPLTCRADSEVRRTVPAQVVVPGLFSPAADDGTH
                     RLVFRHGDDLSPLGTLTDRAAIAAGHISHTILDYTRLGLA"
     gene            1352..>1792
                     /locus_tag="VEA63_02230"
     CDS             1352..>1792
                     /locus_tag="VEA63_02230"
                     /inference="COORDINATES: protein motif:HMM:NF014664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HYD82829.1"
                     /translation="MEKKNVRLKNDDEAAVRREFVRLRKRLAEFDHVDRETRRERGLK
                     KLTEIVGVRGGKVLNELIEFSPELVRFIVDHAYGDVISRERLDGRARALVVIAALAAM
                     GNAAPELRTHVGTALNSGCTREDILETMLLVGVYAGFPAAMNGMG"
BASE COUNT          318 a          432 c          721 g          321 t
ORIGIN      
        1 ggtggagcag cggctgccgt ttttggatca caagctgttc gagacgatca aggacatccc
       61 ggcatcgctg ctatggcgcg acgggcggga gaagtgggtg ctgcgtgagg cgttgaagcc
      121 gtttgtgtcg gacgcggtgt atgcggggaa gaagcagccg ttttcggcgc cgcgatcggc
      181 attgcgcgaa gacagcgcgc tgctcgcgct cgcgcaggaa ttgctgcgta gcgaggcggc
      241 gggaagcgtg ccgtttttgg atcgagcggg attgatcgcg ctcgcggatc gattgatgac
      301 gctgccggat caagagcggt cggcgctcga gtcgaccgtg ctgatgctga tgtcggtcgt
      361 tgtgttacag cagcggtatc gcttgtgacg cgcggaacgg ggcgcggcgc gcatgactcc
      421 gagcggctag gcgaggccga ggcgggtgta gtcgaggatc gtgtggctga tgtgtccggc
      481 ggcgatcgcg gcgcggtcgg tgagcgtgcc gagaggcgag aggtcgtcgc cgtgacggaa
      541 cacgagacgg tgcgtgccgt cgtccgcagc ggggctgaac aagccgggaa cgaccacttg
      601 agcgggcacg gtgcggcgga cctcggagtc ggcgcggcag gtgagcggga aattcacgtt
      661 gtgcacgatc cattttcgcg gcggcgtggc ggcgaggagt tcaggcgcga gccgggcggc
      721 gtggcgcgcg gaggttttga gcgtggcgag cagcgaagcg tcgggcacgc cgccgttttc
      781 tttgaggcgg tcgaaaattt cgaacgagag atcctgcgaa aacgcgatgg cggggagtcc
      841 atggagcgca ccttcccaag cgccggcgat cgtgccgctg gcgatgatga aaccgaggga
      901 gacgttttga ccgacgttga tgccgctgat cacgccgtcg acgggcgtgg tgaggagatg
      961 ggcgaggccg atgttgacgc agtcgctcgg cgtgccgtcg acgatccatg tatcgcaagc
     1021 gagaccacgc tcggagacgc aggcgtgaac gggacggagt ttcgatttcg cggcgccgat
     1081 ccagctttgc tcggttttcg gggcgatgac cgagatgcgg tggccggttt cgcggagcgc
     1141 ggcgaccaat tcgtggagga agcggctgtc gataccgtcg tcgttcgtaa cgagaaaatg
     1201 catggagagg cctgatcaaa caggcgaaag caggggaagg tgaagcgtgg aatcgccgct
     1261 ggaggatttt tcgcgagcgc gggcgacggt ggacggcaca cgacgcggcg cgaaggattg
     1321 cgcggaggtg cgtgcggtgc acgttcgggc catggagaaa aagaatgtgc gtctgaagaa
     1381 cgacgatgaa gcggcggtgc ggcgggagtt tgtgcggttg cgaaaacggc tggcggaatt
     1441 cgaccatgtg gatcgtgaga cgcgccgcga acgcggattg aagaagttga cggagatcgt
     1501 cggcgtgcgc ggcggtaaag tgttgaacga gctgatcgag ttttcgccgg agttggtgcg
     1561 gttcatcgtg gaccacgcat atggcgatgt gatttcacgc gagcgactcg atggtcgcgc
     1621 gcgcgcgctc gtagtgatcg cggcgctggc ggcgatgggc aatgcggcgc cggagttgag
     1681 gacgcatgtc gggacggcgt tgaacagcgg ctgcacgcgc gaggatattt tggagacaat
     1741 gctgctggtg ggcgtgtatg cggggtttcc ggcggcgatg aatgggatgg gg
//
LOCUS       DASPLT010000211         1278 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_268772, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000211 DASPLT010000000
VERSION     DASPLT010000211.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1278)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1278)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1278
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_268772"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            13..93
                     /locus_tag="VEA63_02235"
     CDS             13..93
                     /locus_tag="VEA63_02235"
                     /inference="COORDINATES: protein motif:HMM:NF013151.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD82830.1"
                     /translation="MINEVRGINRVVYDISGKPPATIEWE"
     gene            101..586
                     /gene="tadA"
                     /locus_tag="VEA63_02240"
     CDS             101..586
                     /gene="tadA"
                     /locus_tag="VEA63_02240"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012536323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HYD82831.1"
                     /translation="MGAVQAVDDALYMSEALSLAHQAWQLGEVPVGAVVVHAGQVVGR
                     GFNAPISRNDPTGHAEVMALRDAARNLQNYRLGDCELYVTLEPCVMCAGAIMHARIVR
                     VVYGASDPKTGACGSILDLFAQKRLNHHAEVIGGVMAAECGALLTSFFEQRRRLQKDA
                     R"
     gene            586..1065
                     /locus_tag="VEA63_02245"
     CDS             586..1065
                     /locus_tag="VEA63_02245"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HYD82832.1"
                     /translation="MKIEISIPEQRLTLYGDEGGILKRYSVSTAKNGAGETRGSFRTP
                     RGKHIIRAKIGAGAPENTVFVRRRPTGEIYTPALGAQFPQRDWILTRILWLSGCEPGF
                     NRLGNVDTMRRFIYIHGSPDSVPMGTPGSIGCIRMRNSDLIDLFDRVPVGTPVEIKG"
     gene            complement(1062..>1278)
                     /locus_tag="VEA63_02250"
     CDS             complement(1062..>1278)
                     /locus_tag="VEA63_02250"
                     /inference="COORDINATES: protein motif:HMM:NF013197.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD82833.1"
                     /translation="PAIASGDEIPIEERSAEEVIGYGDVIWAPEGVKVAHPAFDVTPA
                     DLVTALITDKGIVERPNAAKVRELLAR"
BASE COUNT          240 a          402 c          412 g          224 t
ORIGIN      
        1 gtctcgaatc gcatcatcaa cgaagtgcgt ggcatcaacc gcgtcgtgta cgacatttcc
       61 ggcaagccgc ccgctaccat cgagtgggag tagggcgggc atgggcgccg tgcaagccgt
      121 cgacgacgcg ctgtacatga gcgaagcgct ctcgctcgcg catcaggctt ggcaactggg
      181 cgaagtgccg gtcggcgcgg tcgtagtgca tgcgggacag gtcgtcggtc gaggcttcaa
      241 cgcacccatc tcccgcaacg atccgacggg ccacgccgaa gtgatggcgt tgcgcgacgc
      301 ggcgcgcaat ctgcagaact atcgtctcgg cgactgtgag ctgtacgtga cgctcgagcc
      361 gtgcgtgatg tgcgcgggcg cgatcatgca cgcgcggatc gtgcgcgtcg tatacggcgc
      421 aagcgacccg aaaacagggg cctgcggcag catcctcgac ctcttcgcgc agaagcggct
      481 gaatcatcac gcggaagtga tcggcggcgt gatggccgcg gagtgcggcg cgctgctcac
      541 gagttttttc gagcagcggc ggcgtctgca gaaggacgct cggtgatgaa aatcgaaatc
      601 tccatcccgg aacagcggct cacgctctac ggcgacgaag gtgggatcct caagcgctac
      661 agcgtctcga cggcgaagaa cggcgccggc gaaacgcgcg gaagcttccg tacgccgagg
      721 ggcaaacaca tcatacgcgc caagatcggc gccggcgcac cggagaacac cgtcttcgtc
      781 aggcggcggc ccacaggcga aatctatacg cccgcactcg gggcgcagtt tccccagaga
      841 gactggatcc ttacacgcat cctctggctg tcgggctgcg agccgggctt caatcgtctg
      901 ggcaatgtgg acaccatgcg gcgattcatc tacatccacg gcagtccgga ttcggttccc
      961 atgggaacac ccggttcgat cggctgcatc cggatgcgta acagcgatct gatcgatctc
     1021 ttcgatcgcg tgccggtcgg cacgccggtc gaaatcaagg gttagcgcgc gagcagttcg
     1081 cgtaccttcg ctgcattcgg tcgttccaca atgcccttgt cggtgatcag cgccgtcacg
     1141 aggtcggcgg gcgtgacatc gaaagcagga tgcgccacct tgacgccttc cggcgcccag
     1201 atcacgtcac cgtagccgat gacttcctcc gcactgcgtt cctcgatcgg aatctcgtct
     1261 ccgcttgcaa tggcgggg
//
LOCUS       DASPLT010000212         1409 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_166687, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000212 DASPLT010000000
VERSION     DASPLT010000212.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1409)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1409)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1409
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_166687"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1409)
                     /locus_tag="VEA63_02255"
     CDS             complement(<1..>1409)
                     /locus_tag="VEA63_02255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-amylase family protein"
                     /protein_id="HYD82834.1"
                     /translation="VRWGQTNLTEIDPARFDLAWWRAHWKRTALDGVVVNGGGITAYY
                     PTDVPFHRRAEFLGDRDLFGEITRAAHEDGLAVFSRMDSNRADETFYRAHPDWFAHDA
                     EGKPYQWTELYQACVNGPYYDEYMPAVLREIATRYRPEGFTDNNWNGPMRHQPCFCVN
                     CERKFRARTGEGIPRKADWNSPVYREWILWNYERRLEIWDHFNRTTREAGGPHCIWVG
                     MMAGSQNWQARVFRDDREIYRRAELVTLDDQRRHDDEGFQHNGEVGKRIRSAGGWDKV
                     VPESMAMYHLTGHNFRLAAKPTPEVRMWAVEGFAGGVQPWWHVVGSVQEDRRMLETAV
                     PLWQWHREQEAFLVNRRPVATVGLLWSQRNMDFFGRDDGGAHVDEPWNGFTQALVRGR
                     IPYVPVHVDDIEREVAELDLKLLILPNLAALSDAQVAALRRFVKAGGSLLATGLSSLC
                     NEWGDVREDLALADLFGAH"
BASE COUNT          275 a          573 c          318 g          243 t
ORIGIN      
        1 catgcgcacc gaacaaatcc gccaacgcaa gatcctcccg cacgtctccc cattcgttgc
       61 acaacgaact caaccccgtc gcgagcaaac tcccacccgc tttgacgaac cgccgcagcg
      121 ccgccacctg cgcatccgac agcgccgcga ggttcggcaa aatcagcagc ttcaaatcca
      181 gctccgccac ctcgcgctcg atgtcgtcca catgcaccgg cacatacgga atccgcccac
      241 gcaccagcgc ctgcgtaaat ccgttccacg gctcatcgac gtgcgcgccg ccgtcgtcgc
      301 gcccgaaaaa atccatgttc cgctgcgacc acaacaatcc caccgtcgcg accggtcgcc
      361 gattcaccaa gaacgcctct tgctcgcgat gccactgcca caacggcacc gccgtctcca
      421 gcatccgccg atcctcttgc accgacccga cgacatgcca ccacggctgc acgccgcccg
      481 cgaacccttc caccgcccac atccgcacct ccggcgtcgg tttcgccgcg agccggaaat
      541 tgtgtccggt taaatgatac atcgccatgc tctccggcac gactttgtcc caaccgccgg
      601 cgctgcgtat ccgttttccc acttcaccgt tgtgctgaaa cccttcgtcg tcgtgacgcc
      661 gttgatcatc cagcgtcacc agctccgcgc gccgatagat ttcccgatca tcccgaaaca
      721 cccgcgcctg ccagttctgc gagcccgcca tcatccccac ccagatgcaa tgcgggccac
      781 ccgcctcgcg cgtcgtgcgg ttgaaatggt cccaaatctc cagccgccgc tcgtagttcc
      841 ataaaatcca ctcgcgatac accggcgaat tccagtccgc cttccgcgga atcccctcac
      901 ccgtccgcgc gcgaaacttc cgctcgcagt tcacgcagaa acacggctga tgccgcatcg
      961 gtccgttcca gttgttgtcc gtaaaacctt ccggccggta acgcgtcgcg atctcccgca
     1021 acaccgccgg catatactcg tcgtaatacg gcccgttcac gcacgcctga taaagctccg
     1081 tccactgata cggtttcccc tccgcatcat gcgcgaacca gtccggatgc gcccgataaa
     1141 acgtctcatc cgcgcggttc gagtccatcc gcgaaaacac cgccagcccg tcctcgtgcg
     1201 ccgcgcgcgt gatctccccg aataaatcgc gatcgcctaa aaactccgcg cgccggtgaa
     1261 acggcacgtc cgtcggataa tacgccgtga tgcctccgcc gttcaccacc acgccgtcca
     1321 gcgccgtccg cttccaatgc gcccgccacc acgccagatc gaaccgcgcc ggatcgatct
     1381 ccgtcagatt cgtctgcccc caccgcaca
//
LOCUS       DASPLT010000213         1672 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_227740, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000213 DASPLT010000000
VERSION     DASPLT010000213.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1672)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1672)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1672
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_227740"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            217..1149
                     /locus_tag="VEA63_02260"
     CDS             217..1149
                     /locus_tag="VEA63_02260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HYD82835.1"
                     /translation="MMTRASFVAVGVFSLATTAALAAAEPRVIPLWPDRPPGETAALP
                     PEADITTADDRQPAGRRVTRISNVSHPTLTIYPPDPAIDTRTAVLVCPGGGYARLAID
                     IEGTEVCDWLNSIGVTGIILKYRVPRREGVAQHLPPAQDAQRALGLLRHRAPELGLDP
                     HRIGIIGFSAGAHVAGVLSTHQDERLYPTVDAADRVSCRPDFAMLIYPGYFTARDREF
                     GIAPEVAVAADKTPPTFIAMAQDDPVRVENALYYYLALKQVGVPAEMHLYPTGGHGFG
                     LRATGEPAATWPARAADWLRASGWLKRDPASGAN"
     gene            1413..>1672
                     /locus_tag="VEA63_02265"
     CDS             1413..>1672
                     /locus_tag="VEA63_02265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ThuA domain-containing protein"
                     /protein_id="HYD82836.1"
                     /translation="MITPPALRTLLTSLVFACCSTLAAATAPHKALILDGQNNHDWAV
                     STPLLKNYLEQTGLFTIDVATSPPKGADMSTYRPAFANYAVVV"
BASE COUNT          299 a          651 c          416 g          306 t
ORIGIN      
        1 agcgcgagcg ctacacacaa acgaaacaag gttttcaggg ggtgaaaatt cgccgcacgg
       61 cggtcgggct tggtgggatc gccggcagac gaagccgtca gcaaagcccg cacccgccct
      121 ggcgcaagct tcgcgaccca cgccgcccgt tattcgcgct ccgctttttt tcttcgcctc
      181 cccatcacgt tctcatccgt cttgccttaa caccccatga tgactcgcgc ctccttcgtc
      241 gccgtcggcg ttttctcgct cgccaccacc gccgccctcg ctgccgccga gccgcgtgtt
      301 attccgctct ggcccgaccg ccctcccggc gaaaccgccg cacttccgcc cgaagccgac
      361 atcaccacag ccgatgaccg tcagcccgcg ggccgccgcg tcactcgcat cagcaacgtt
      421 tcccatccca cgctcacgat ttatccgccc gatcctgcga tcgacacccg caccgccgtg
      481 ctcgtgtgtc caggcggcgg atacgctcgc ctcgcgatcg acatcgaagg caccgaagtg
      541 tgcgactggc tcaactcgat tggcgtcacc ggcattattc taaaatatcg cgtgccccgc
      601 cgcgaaggcg tcgcgcaaca cctgccgccc gcccaagacg ctcaacgcgc gctcggcctc
      661 ctgcgtcatc gcgcgccgga actcggcctc gatccgcacc gcatcggcat cattggtttt
      721 tccgccggcg cacatgtggc cggcgtgctc agcacccatc aggacgaacg tctttatccc
      781 accgtcgacg ccgccgaccg cgtgagctgc cgccccgatt tcgcaatgtt gatctacccc
      841 ggctatttca ccgcacgcga ccgcgagttc ggcatcgctc cggaggtggc cgtcgccgcc
      901 gacaaaacgc cgccgacctt catcgccatg gcgcaggacg atccggtccg ggtcgaaaac
      961 gcgctttact actacctcgc gctcaagcag gtcggcgttc ccgccgagat gcatctgtat
     1021 ccaaccggcg gacacggctt cggccttcgc gccaccggcg aacccgccgc cacctggcct
     1081 gcccgcgccg ccgactggct gcgcgcctcc ggctggttaa aacgcgatcc cgcgtccggc
     1141 gcgaactgac cgaacacgac agtctgaaac caccgatgaa cacagatgga cgccgatcct
     1201 aaatttcctc cgtatccgtg ttcatccgtg tccatccgtg gttgtaacgg atcggatttt
     1261 tgaaccacca atggacacga agagcacaaa ttcaaaccag cagccgccca cgacttcttg
     1321 cactccattc gtgttcattc gtgtccattc gtggttcaac tgaattgaac cgggctgccc
     1381 aaacgccgct taccatccct cctcacgtcg tcatgataac cccacccgcc cttcgcaccc
     1441 tgctcacgtc gctcgtcttc gcctgctgct cgactctcgc cgccgccacc gcgccgcaca
     1501 aagccctcat cctcgacggc cagaacaacc acgactgggc cgtgagcacg ccgctgctga
     1561 aaaattatct cgagcaaacc ggcctcttca ccatcgacgt cgcgacctcg ccgcccaaag
     1621 gcgccgatat gagcacctat cgccccgcgt tcgccaatta cgccgtcgtc gt
//
LOCUS       DASPLT010000214         2797 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_3089, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000214 DASPLT010000000
VERSION     DASPLT010000214.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 2797)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2797)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2797
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3089"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..301
                     /locus_tag="VEA63_02270"
     CDS             <1..301
                     /locus_tag="VEA63_02270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD82837.1"
                     /translation="LFSALEHERVPLRVLLRTPEATHLRAFTPENVILDQQLLTVAGE
                     RTTLQLALPAVRTLHLAPDHSLHVGGPGRTLLLSLAAADGESPRWSSALDALFEI"
     gene            316..786
                     /locus_tag="VEA63_02275"
     CDS             316..786
                     /locus_tag="VEA63_02275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007363790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin domain-containing protein"
                     /protein_id="HYD82838.1"
                     /translation="MRPTLALYYATVTGNAESLARQAETRALNEDWLPRLQNLSELKP
                     ADLAGDRLALFIVSTWGDGEPPVDAADFWSDLAQSTPDLSGLHYAVLGLGDRDYAEFN
                     AFARALDERLATLGAHRLHERIEADAYFDDTYAEWESRVFPLLAAHRAVDALAR"
     gene            913..1605
                     /locus_tag="VEA63_02280"
     CDS             913..1605
                     /locus_tag="VEA63_02280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD82839.1"
                     /translation="MKNLVIRSPLCALVGALFIALPCLRAHSVWLEDTPDQQLVLRFG
                     EPGDDYEKSPGHLDSLTLPSAWTLKSDDDGKPAAFTVEKKSDHFLFASADPSIAAFGE
                     TRFPVRKRGNRPASWPHFYVRWQPAGAPTAATLSPTLTLDIVPTLTPGEFRVHFRGQP
                     IAGATVTARHFGTHAEEKLTADDRGIIRFTTTDPGLVLLTANHKEPLAGFSGGVAYDV
                     TSHNTALTWRQP"
     gene            1618..2790
                     /locus_tag="VEA63_02285"
     CDS             1618..2790
                     /locus_tag="VEA63_02285"
                     /inference="COORDINATES: protein motif:HMM:NF042665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6607 family protein"
                     /protein_id="HYD82840.1"
                     /translation="MKSFLLPSLLCASILLATGCQHAAPPTPAPPAAASVRANTHPTP
                     AAADELSYLFGWGDVPATVANPRGGTSRGAPVTLAPPQTLPHPAIAAATSAFERDRAA
                     ILALAGDYRVSFHFMETLGLTTDHAPKSPYHSWATEHVRVVTDTGRFISLQHTLVMFM
                     KKDDGTASDPMVMKHWRQDWTYEDTDLHTYVGDRTWARRRLSPADAAGTWAQAVFQVD
                     DSPRYEAFGRWEHRGNLSLWTGSREFRPLPRREHTVRKDYSIMEGSHRIILSPTGWLH
                     EQHNWKRVANDPASEPRYLAQELGLDRYERISSPSLAAADASFEKTEPYWSIVRRVWS
                     EIFATRDRFTLRTDVHGKRLFEEHFAYAEKLASGEPFDAADAERHARETIASFLVP"
BASE COUNT          477 a         1181 c          669 g          470 t
ORIGIN      
        1 tctcttctcc gcactcgaac acgaacgcgt gccgcttcgc gttctgctgc gcacgcccga
       61 ggcgacgcac ctgcgcgcct tcactcccga aaacgtcatc ctcgaccagc aactcctcac
      121 cgtcgccggt gagcgcacca cgttgcagct cgcgctgccc gccgttcgca cgctccacct
      181 cgcacccgac cacagcctcc acgtcggcgg ccccggccgc acgctgctcc tctcgctcgc
      241 cgccgccgac ggcgaatcgc cccgctggtc ctccgccctc gacgcccttt tcgaaatcta
      301 aatccacccg ccgccatgcg cccaaccctc gccctctact acgccaccgt caccggcaac
      361 gccgaatccc tcgcccgcca ggcggaaacc cgcgcgctca acgaggactg gcttccccgc
      421 ctgcaaaacc tttccgagct caagcccgcc gacctcgccg gcgaccgcct cgccctcttc
      481 atcgtttcca cctggggcga tggcgaacca cccgtcgacg ccgccgactt ctggtccgac
      541 ctcgcgcaat ccacgcccga cctctccggt ctccattacg ccgtcctcgg cctcggtgac
      601 cgcgactacg ccgagttcaa cgccttcgcc cgcgccctgg acgaacgcct cgccacgctc
      661 ggcgcccacc gtctccacga acgcatcgaa gccgacgctt acttcgacga cacctacgcc
      721 gaatgggaat cgcgtgtgtt tcccctcctc gccgcgcatc gcgccgtcga cgccctggcc
      781 cgctgattac cgcgtatcca aaattctccc acctgcgcca tggcgccaac cgcgcatgtt
      841 gtggctcgga aatccacacc agccgaaaac gccgggtgcg ggtgggaccc ttttcctttc
      901 cctcttccaa ccatgaaaaa cctcgtgatc cgctccccgc tctgcgccct cgtcggcgca
      961 ctcttcatcg ctctgccttg ccttcgcgcc cactccgtct ggctcgaaga cacgcccgac
     1021 caacaactcg tcctgcgctt cggtgaaccc ggcgacgact acgagaaatc ccccggacat
     1081 ctcgacagcc tcacgctccc ttccgcgtgg acattgaaat ccgacgacga cggaaaaccg
     1141 gccgccttca ccgtcgagaa aaaatccgac cacttcctct tcgcctccgc cgatccttcc
     1201 attgccgctt tcggcgaaac ccgcttcccc gtccgtaaac gcggcaatcg tccggcgagc
     1261 tggcctcact tctatgttcg ctggcagcca gccggcgcgc ccaccgccgc cacgctctcc
     1321 ccaacactca cgctcgacat cgtgcccacc ctcacgcccg gcgagtttcg cgttcacttc
     1381 cgcggccagc cgatagccgg cgcgaccgtc accgcccgcc atttcggcac gcacgccgag
     1441 gaaaagctca ccgccgacga ccgcggtatc attcgtttca ctacaaccga tcccggcctc
     1501 gtcctgctca ccgcgaatca caaggagccg ctcgctggtt tttccggcgg cgtcgcctac
     1561 gacgtgacga gccacaacac cgcgctcacc tggcgccagc cctgaaccct cgctccgatg
     1621 aaatcctttc tcctaccctc gctgctttgc gcttcgatcc tgctcgccac cggctgccaa
     1681 cacgccgcgc ctcccacacc tgcgccaccg gcagctgcga gcgtgcgtgc caacacgcac
     1741 cccacccctg cagctgcgga cgaattgtcc tatcttttcg gctggggcga tgttcccgcc
     1801 accgtcgcca accctcgcgg aggcacctcg cgcggcgcac ccgtcacgct cgccccgccc
     1861 caaacgcttc cgcaccccgc catcgccgcc gcgacttccg ctttcgaacg cgaccgcgcc
     1921 gcgatcctcg cgctcgccgg cgactaccgc gtcagctttc acttcatgga aacgctcggc
     1981 ctcaccaccg accacgcgcc gaaatcgcct tatcactcgt gggccaccga gcacgtccgc
     2041 gtcgtcaccg acaccggccg tttcatcagc ctccagcaca cgctcgtcat gttcatgaaa
     2101 aaagacgacg gcaccgcctc cgacccgatg gtcatgaaac actggcgaca ggattggacc
     2161 tacgaggaca ccgatctcca cacctacgtt ggcgatcgca cgtgggctcg ccgccgcctc
     2221 tcgcccgctg acgccgccgg cacctgggcg caagcggtct tccaagtcga cgactccccg
     2281 cgctacgaag ccttcggccg ttgggaacac cgcggcaatc tctcgctctg gaccggctcg
     2341 cgcgaattcc gtccgctccc gcgtcgcgaa cacaccgtgc gcaaagacta ctccattatg
     2401 gaaggctcgc accgtatcat tctctcgccg accggctggc tccacgagca gcacaactgg
     2461 aaacgcgtgg ccaacgaccc cgccagcgaa ccgcgctacc tcgcacaaga gctcggcctc
     2521 gatcgctacg aacgcatctc atcgccaagc ctcgccgccg ccgacgcctc cttcgagaaa
     2581 accgaaccct actggtccat cgtccgccgc gtctggtccg agatcttcgc cacgcgcgat
     2641 cgtttcacgc tccgcaccga cgtccacggc aaacgcctct tcgaagaaca tttcgcctac
     2701 gccgaaaaac tcgcttccgg cgaacccttc gacgccgccg acgcggaacg ccacgcgcgc
     2761 gaaaccatcg catcgttcct cgtgccttga tcgttcg
//
LOCUS       DASPLT010000215         1194 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_268812, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000215 DASPLT010000000
VERSION     DASPLT010000215.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1194)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1194)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1194
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_268812"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            242..>1194
                     /locus_tag="VEA63_02290"
     CDS             242..>1194
                     /locus_tag="VEA63_02290"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HYD82841.1"
                     /translation="MLDAGHLPALASLIQSGSSGSIRGFAPWLPPVSWTTLATGAHPD
                     RHGIHGVAVFDEAVGRPEAAGTRHRRLPAIWNRLSAHGHSCAVLGWPGTYPAEVLPGG
                     IMVSDGFAHAPLGPDDPWPVPAGAVSPVARAADLAELRLRPSEIEPGLLGLFLPKLNA
                     IDLANDSRPGWLLQQLSELYALHNTAVVLCGEKQPDFLAVHFPFIATVQKEFGLFQAP
                     AHPAASAVDRERYSDVVSSAYRVQDALLADLINACEPGTAIVVVSAHGLAHGADRPAR
                     RPRSAVEYAAWCRPHGLLVMKGPGLRAGAIIANAHIEDLAPT"
BASE COUNT          176 a          410 c          372 g          236 t
ORIGIN      
        1 tcgacacctc cggtgccccc atcgcagtgg gatcgatcat gtcgtccatt cctgaacctt
       61 ccactaccgt gctttcgatg ggcgccgccg ccttgctagc cggctcggca gccgcctggc
      121 gtcgccgcaa gggacggttg cccgtcgcgg cttgagcgag agacctccag ttccttctcc
      181 ctccgccaaa aaaattgtcg tcgtaggctg ggaaggagcg gattggtcac acgcgcaacc
      241 cttgctggac gcgggccatc tgcccgcact ggcctcgttg atccaatcgg gcagctccgg
      301 ctccattcgc ggttttgcac cgtggctacc gcccgtttcg tggaccacgc tcgccaccgg
      361 tgcgcacccg gaccggcatg ggattcatgg tgtcgccgtc ttcgatgagg ccgttggccg
      421 tcccgaagca gccggcaccc gccatcgtcg gcttccggca atttggaatc gtttgtccgc
      481 gcatggccac tcatgcgccg tgctcggctg gcctggcact tatccggccg aagtcctgcc
      541 gggcggcatt atggtcagcg acggtttcgc ccacgctcca ttgggaccgg atgacccctg
      601 gcctgtcccc gccggagcgg tgagtcccgt ggcacgcgcg gccgatctgg cagaactgcg
      661 actacgtccc tccgaaatcg agcccggctt gctcgggctc tttcttccga agttaaacgc
      721 tatcgatctg gcgaacgatt cccgaccggg ctggttgctt cagcaactct cggagctcta
      781 cgcgctccac aacacggcgg tcgtgctgtg cggcgaaaag cagcccgact tcctcgcggt
      841 tcacttcccg ttcatcgcga cggtgcagaa agagttcggg ctctttcagg cgccggctca
      901 tccggcggcg agcgcggtcg atcgcgaacg ctatagcgac gtcgtttcgt ccgcctatcg
      961 ggtgcaggat gcgttgctcg cggatttgat caacgcgtgt gaacccggca ccgcgatcgt
     1021 ggtggtttcc gcgcatggcc tcgcgcacgg tgccgaccgc ccagcgcgcc ggccgcgcag
     1081 cgcggtcgag tatgctgcgt ggtgccgtcc gcacggcttg ctggtgatga aaggaccagg
     1141 actgcgggcg ggcgcgatca tcgcgaacgc tcacatcgag gatctcgcac ccac
//
LOCUS       DASPLT010000216         1422 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_63878, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000216 DASPLT010000000
VERSION     DASPLT010000216.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1422)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1422)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1422
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_63878"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..364)
                     /locus_tag="VEA63_02295"
     CDS             complement(<1..364)
                     /locus_tag="VEA63_02295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HYD82842.1"
                     /translation="MKALLIDDERLARNELRRLLAAFPELEIVGEAANARQAREQIAQ
                     HSPELIFLDVQMPGETGMELLESLEPPAPEVIFTTAYDEFAVKAFDLNALDYLLKPVD
                     PARLAVAIEKLRAKRGAGG"
     gene            complement(372..>1422)
                     /locus_tag="VEA63_02300"
     CDS             complement(372..>1422)
                     /locus_tag="VEA63_02300"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF018305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HYD82843.1"
                     /translation="FQTLGWGFLLVVQLVVSVYFATRDGKKDPVTAASTVVMVMALGL
                     LLSHYFRPFMSRWSWKQLGWRQLLPRVLMASVVLGVLWNGIGYAIAYGLLDQPWEGKH
                     NRVVIFALGSLNGALIFVGWMCLYFFYHLFDRFNRSEIERLRLTTHVKDAELRALKSQ
                     VNPHFIFNSLNSLRALIEEDPARARQTVTQLANLLRYSLQSGQLETVPFEDELRVASD
                     YLALEQVRHEERLRVRLDIAPDTLGLPIPPMLLQTLVENAVKYGISPRAEGGEVAIIA
                     RRENDALRLQVTNPGDLNATDPARAASSTGLGLRNAAERLRLLFGERATLQLRAGDAA
                     TVVAEAIVPLGLARG"
BASE COUNT          293 a          494 c          403 g          232 t
ORIGIN      
        1 cgccgccagc gccgcgcttc gcgcgcaact tctcgatcgc gaccgcgaga cgcgccggat
       61 cgaccggctt caacaagtaa tcgagcgcgt tcagatcaaa cgccttcacc gcaaactcgt
      121 cgtaagccgt cgtgaaaatc acctcgggcg ccggcggctc gagcgattcg agcagctcca
      181 tgcccgtttc tcccggcatc tgcacgtcga gaaaaatcaa ctccggcgaa tgttgcgcga
      241 tctgctcgcg cgcctgccgc gcgttcgccg cctcgcccac aatttccagt tcgggaaagg
      301 ccgcaagcag gcgccgcagt tcgttgcgcg cgagccgttc gtcatcgatg aggagtgctt
      361 tcatgctgaa atcatccgcg cgccaaaccg agcggcacaa tcgcttcggc cacaaccgtc
      421 gctgcatcgc cggcgcgaag ctgcaacgtc gctcgttcgc caaacaacag ccgcagccgc
      481 tccgccgcat tgcgcaaacc gagcccggtc gacgacgccg cgcgcgccgg gtccgtcgcg
      541 ttgagatcgc cgggattcgt gacctgcaac cgcaacgcgt cgttctcgcg gcgcgcgatg
      601 atcgccacct cgccgccttc cgcacgcggc gagattccgt atttcaccgc gttctccacc
      661 agcgtttgca gcagcatcgg cgggatcggc aggccaagcg tgtcgggcgc gatgtcgaga
      721 cggacgcgca ggcgctcttc atgccgcact tgctcgagcg cgagataatc gctcgccacg
      781 cgcagctcgt cctcgaaggg caccgtctcg agctgcccac tctgaagcga gtagcgcagc
      841 agattcgcca gttgcgtcac cgtctgccgc gcgcgcgccg gatcttcctc gatcagcgca
      901 cgcagcgagt tgagcgagtt gaagatgaag tgaggattca cctgcgattt caacgcgcgc
      961 agctccgcgt ccttcacatg cgtcgtcaac cgcagtcgct cgatctccga gcgattgaaa
     1021 cgatcgaaca ggtgatagaa aaaatacaga cacatccagc cgacgaagat cagcgcgccg
     1081 ttcaggctgc ccagcgcgaa gatcaccacg cggttgtgtt tgccctccca aggctgatcg
     1141 agaagcccat aggcgatggc ataaccgatc ccgttccaaa ggacgcccag cacgaccgac
     1201 gccatcaaca cgcgcggcag cagttgacgc cagccgagct gtttccaact ccaccgcgac
     1261 atgaagggac ggaagtagtg gctcaacagc agacctagcg ccatcaccat gaccacggtc
     1321 gacgccgcgg tcactggatc tttcttcccg tcgcgcgtgg caaaatagac cgaaaccacc
     1381 aactgcacga cgaggaggaa accccagccc agcgtttgaa ac
//
LOCUS       DASPLT010000217         1478 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_166709, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000217 DASPLT010000000
VERSION     DASPLT010000217.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1478)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1478)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1478
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_166709"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..496
                     /locus_tag="VEA63_02305"
     CDS             <1..496
                     /locus_tag="VEA63_02305"
                     /inference="COORDINATES: protein motif:HMM:NF013355.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD82844.1"
                     /translation="NLDTLRPEPTVEGFTTPGGYSSRAVKPIALRMVMEVAQLIRSEF
                     PGRSLSGLGGIESGRDAAEFILLGADTVQVCTGVMKSGYECVKPMCEELLAFMAKHQF
                     ETLADFRGKSLDYFTTHAALVQKQTERKAAQKAAAATKVVKNDAEWTGDEFVKQSDAL
                     SRGA"
     gene            529..1005
                     /locus_tag="VEA63_02310"
     CDS             529..1005
                     /locus_tag="VEA63_02310"
                     /inference="COORDINATES: protein motif:HMM:TIGR02436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HYD82845.1"
                     /translation="MKLQASRSKLDGRSKLQGGQKPLRAREEYPEPSSFSWLLNESEG
                     RHPFDLEERTAVFGEAIIGFVKKIPRHPANDRLINQLVGCGTSIGANYCEANEGVSKK
                     DFRNIVGRCVKEAKETKFFLRMIAAAEPKLAEEARALYREARELHLIFAAIYRKAG"
     gene            1118..>1478
                     /locus_tag="VEA63_02315"
     CDS             1118..>1478
                     /locus_tag="VEA63_02315"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HYD82846.1"
                     /translation="MIAVPHLIAGAWRPDSPLPPTPVYNPSVGEIIAECPAADAALVD
                     EAVSAAHAAFPAWRDTPAVERARLFFRYRQLVEQNFDQLCHTVSREHGKTHAEARGSV
                     HRGLENIEYACGVPSLLF"
BASE COUNT          314 a          499 c          398 g          267 t
ORIGIN      
        1 gaacctcgac acccttcgcc ccgagcccac cgtcgaaggt ttcaccacgc ccggtggtta
       61 ttcgtctcgc gcggtcaaac cgatcgcgct ccgcatggtg atggaagtcg cgcagctcat
      121 ccgcagcgag ttcccgggac gttcgctctc cggcctcggt ggaatcgaga gcggccgcga
      181 cgccgccgag tttatcctcc tcggcgccga caccgtgcaa gtgtgcaccg gcgtgatgaa
      241 gtccggttac gaatgcgtga agccgatgtg cgaagagctc ctcgcgttca tggcaaagca
      301 ccagttcgaa acgctcgccg atttcagagg caagagcctc gactacttca cgactcacgc
      361 cgcgctcgtc caaaaacaaa ccgaacgcaa agccgcccaa aaagccgccg ccgcaacgaa
      421 agtggtgaag aacgacgccg aatggaccgg cgacgagttc gtgaagcaat ccgacgcgct
      481 ttcacgcgga gcgtgaaaga agctccaaga tccaagctcc aagcaccagt gaaactccaa
      541 gcttcaagat ccaaactcga cggacgctcg aagcttcaag gggggcaaaa gccactccgt
      601 gcacgcgaag agtatccaga gccttcgtcg ttttcgtggc tgctcaacga aagtgaaggt
      661 cgccatccct tcgacctcga ggagcgaacc gccgtctttg gtgaagcgat catcgggttc
      721 gtgaagaaga tcccgcgtca tcctgcgaat gatcgcttga tcaaccagct tgtcggctgt
      781 ggcacgagca tcggcgctaa ctactgcgaa gcgaatgagg gtgtttcaaa aaaggatttc
      841 cgaaacatcg tcggacgctg cgtgaaagag gcaaaggaaa cgaagttctt tctgcggatg
      901 atcgccgccg ccgagcccaa gctcgctgaa gaagctcgcg ccctttaccg cgaagcccgg
      961 gaactccacc tcatcttcgc cgcgatctac cgcaaagccg gttgaccatc ctaatgagtg
     1021 cgcgaatccc gtccatcccg ccgctcgccc caatattgaa gcttgtggct tggagtttca
     1081 ctggagcttg gaggttggag cttggagctt ttccccaatg atcgccgttc ctcatctcat
     1141 cgccggcgcc tggcgccccg actcccccct gccgcccacg cccgtctata acccgtctgt
     1201 gggggagatc atcgccgaat gccccgccgc cgacgctgcg ctcgtcgacg aagccgtctc
     1261 cgccgcgcac gccgccttcc ctgcttggcg cgacacaccc gccgtcgaac gcgcccggct
     1321 ctttttccgc tatcgccaac tcgtcgaaca aaacttcgac caactctgcc acaccgtttc
     1381 gcgcgaacac ggcaagactc acgccgaagc gcgcggcagc gttcaccgcg gtctcgaaaa
     1441 catcgaatac gcctgcggcg tcccttcgct cctcttcg
//
LOCUS       DASPLT010000218         1037 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_186829, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000218 DASPLT010000000
VERSION     DASPLT010000218.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1037)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1037)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1037
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_186829"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..270)
                     /locus_tag="VEA63_02320"
     CDS             complement(<1..270)
                     /locus_tag="VEA63_02320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007361271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HYD82847.1"
                     /translation="MTSWKNQYGRAAPWFLLPALALLAVFVVWPMARAAWWSLTNADL
                     LAPGDARWIGLGNYSDLLGDPRFRRAFANTALFAVMVVPVQTAVAF"
     gene            complement(409..>1037)
                     /locus_tag="VEA63_02325"
     CDS             complement(409..>1037)
                     /locus_tag="VEA63_02325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009510628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HYD82848.1"
                     /translation="SNGHTVRGVLASEANRESLRAWQTLFTEGYAATDPVDPDPFGND
                     STAMDWSGHWMARSHVAAKGERLGVMPLPRLGSKPVAPCGSWCWTVSSAASDIELAAL
                     WVRWVTDARHGVEPIVRANGAVPARGSAFAAFPEYAELPYRLFRDQLENFARPRPRTP
                     HYATLTQRFAGALRDIARGADVATRLRAAETEIQRVIDRREVPSHAAE"
BASE COUNT          181 a          316 c          378 g          162 t
ORIGIN      
        1 aaatgcgacg gcggtttgca ccggcaccac catcacggcg aacagcgcgg tgttggcgaa
       61 cgcgcgacgg aagcggggat cgccgagcag atcggagtaa ttgccaagcc cgatccagcg
      121 cgcgtcaccg ggcgcgagca gatcggcatt cgtcaggctc caccaggcgg cgcgcgccat
      181 tggccacacg acgaagacgg cgaggagcgc gagagccggc agcagaaacc acggagcggc
      241 gcggccgtat tggtttttcc atgacgtcat ggcgcggggc tgtcgggtga aagtgacggc
      301 gtttgtttcg aaatcgagcg ctgcgagcgg gcgatcccgc tgctcacgca gcgagccacg
      361 cgggatggcg tggctttgcg cgtgagctca gggaggaaag aattcatgtt actccgcggc
      421 atgggaggga acttcgcggc gatcgatgac gcgttggatt tcggtttcgg cggcacgcag
      481 ccgggtggcg acgtctgcgc cgcgcgcgat gtcgcggagc gcgccggcga agcgttgcgt
      541 cagcgtggcg tagtgcggcg tgcgtgggcg cggacgagcg aagttttcca gctggtcgcg
      601 aaaaagccga tacggcagct cggcatactc gggaaacgcg gcgaatgccg atcctcgcgc
      661 cggcacggcg ccgttggcgc gcacgatcgg ttcgacgccg tgacgcgcat cagttaccca
      721 gcgcacccat agcgcggcga gctcgatgtc gctcgccgcc gaggaaacgg tccagcacca
      781 acttccgcac ggcgcgacgg gtttcgaacc gagtcgcggg agcggcatca caccgaggcg
      841 ttcgcctttc gccgcgacgt gcgaacgcgc catccagtgt ccgctccaat ccatcgcggt
      901 gctgtcgttg ccgaacggat cgggatcgac gggatcggtc gccgcgtagc cttcggtgaa
      961 gagcgtttgc caggcgcgga ggctttcgcg attggcttca gacgcgagca cgccgcggac
     1021 ggtgtgacca ttgctcg
//
LOCUS       DASPLT010000219         1577 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_125361, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000219 DASPLT010000000
VERSION     DASPLT010000219.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1577)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1577)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1577
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_125361"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1204)
                     /locus_tag="VEA63_02330"
     CDS             complement(<1..1204)
                     /locus_tag="VEA63_02330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012377309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HYD82849.1"
                     /translation="MQQPPASDRLSTREYVGYALGDTASNLFFQTFNIFLTYYYVDVW
                     GIPAAALLIMMPLVRLWDAINDPMMGVIADRTNTRWGKFRPYLLWGAVPYGICGYLMF
                     AGPDLSADGKLVYAYVTYTLMLMAYTVINVPYSSLLGVISPSSRTRTVASSFRFVGAF
                     GGGLLITLLARPLVKALGGDNEVLGFQYTMALFGLLSVIMFWFCFATTKERVSPPPQQ
                     KTNLVGELKELMRNWPWMVLLFVAIFSTTFIALRSGSTLFYFKYVGGDDGSSVFYGLD
                     RSTVFLSTGMLAMMLGTTCLGVFARKLDKKTLAAWLSAITGVSFIAFFFLPVDNFWLL
                     VGVNAVGTFCMGPTSALVWAMYADVADYGEWKFNRRSTGLVYSASLFALKTGIMLGGF
                     ILPLFLDQF"
     gene            1351..>1577
                     /locus_tag="VEA63_02335"
     CDS             1351..>1577
                     /locus_tag="VEA63_02335"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HYD82850.1"
                     /translation="MSGSVAHMNSETRKTISLSQRRLGAAGPLVAEIGLGCMGMSEFY
                     QPAAMNDEESIRVIHRFLDAGGNFLDTADMYG"
BASE COUNT          362 a          385 c          581 g          249 t
ORIGIN      
        1 cgaattgatc gagaaagagc ggaagaatga agccgccgag catgatgccg gttttcaaag
       61 cgaagagaga tgcggagtaa acgaggccgg tggagcgccg gttgaacttc cattctccgt
      121 agtcggcgac gtcggcatac atcgcccaga cgagcgcgga cgtggggccc atgcagaacg
      181 tgccgacggc gttgacgccg acgaggagcc agaagttgtc gacgggcagg aagaagaagg
      241 cgatgaacga aacgccggtg atggcggaga gccacgcggc gagcgttttc ttgtcgagct
      301 tgcgggcgaa cacgccgagg caggtggtgc cgagcatcat cgcgagcatg ccggtggaga
      361 gaaaaacggt gctgcgatcg agaccgtaga aaaccgagga gccatcgtcg ccgccgacgt
      421 atttgaaata gaagagcgtg ctgccggagc ggagcgcgat gaacgtcgtg gagaagatgg
      481 cgacaaagag gaggaccatc catggccagt tgcgcatgag ctccttcaac tcaccgacga
      541 ggttggtttt ctgctgcgga ggcggggaaa cgcgttcctt ggtggtcgcg aagcagaacc
      601 aaaacatgat gacggagagc aggccgaaga gcgccatcgt gtattggaag ccgaggactt
      661 cgttgtcgcc gccgagggct ttgacgagcg gccgggcgag gagcgtgatg agaagaccgc
      721 cgccgaaagc accgacgaaa cggaagctcg aggcgaccgt gcgcgtccgc gatgacgggc
      781 tgatgacgcc gaggagcgag gagtaaggaa cgttgatgac ggtgtaggcc atcaacatga
      841 gcgtgtaagt gacgtaggcg tagacgagtt tgccgtcggc cgagagatcg gggccggcga
      901 acatcaggta gccgcaaatg ccgtagggca cggctcccca gagaaggtag gggcggaatt
      961 ttccccagcg ggtgttggtg cgatcggcga tcacgcccat catcggatcg ttgatcgcgt
     1021 cccagaggcg gacgagcggc atcatgatca gcagcgcggc ggccgggatg ccccacacat
     1081 ccacatagta gtaggtgagg aaaatattaa atgtctggaa gaacaggttc gaggcggtgt
     1141 cgccgagcgc gtagccgaca tattcgcgcg tggagagacg gtcggaggca gggggttgct
     1201 gcatgcgcgc tcaggaaacg agcggcccgc ggcgccgaca aggctcgatt gccggcgggc
     1261 cgtcgaaatg atgacacgcc gcgcgtgcaa ccgaacggtg tgcgttgcga cgggggaagg
     1321 aaatacgaag gggcggttga agttcggcgc gtgagcggct cagttgcgca catgaactcg
     1381 gaaacgcgga aaacaatttc gttgtcacag cggcgattgg gcgcggcggg gccgctggtg
     1441 gcggagatcg ggctcggctg catgggcatg agcgaatttt atcagccggc ggcgatgaac
     1501 gacgaggagt cgatccgcgt gattcaccgt ttcctggacg caggtggtaa ttttttggat
     1561 acggcggaca tgtatgg
//
LOCUS       DASPLT010000220         1009 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_24968, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000220 DASPLT010000000
VERSION     DASPLT010000220.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1009)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1009)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1009
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_24968"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..728
                     /locus_tag="VEA63_02340"
     CDS             <1..728
                     /locus_tag="VEA63_02340"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HYD82851.1"
                     /translation="RPGDRVAALSQNAFAEYDYCAAAEVAPLPASLATKPFPGEAIGC
                     AMNVFRRSDIRPHHHVAIVGLGFLGALLTQLAARTGARVYAISRRPFAQEVAKKMGAT
                     ATFGMSDTWATIEEIKQLTERAGCERVIEATGLQAPLDLATELTAERGKLIIAGYHQD
                     GLRQVNMQLWNWRGLDVINAHERDPRIYLQGMQEAADAVASGRLDPTPLYTHRYSFDE
                     LPRALNQMRDRSDGFLKALVTYE"
     gene            825..>1009
                     /locus_tag="VEA63_02345"
     CDS             825..>1009
                     /locus_tag="VEA63_02345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD82852.1"
                     /translation="MTLQPEPLLTVETPLPRTPRLGFAGVGWIGRNRLQAIATSGLAE
                     IAAIADSAAGAIAETRP"
BASE COUNT          176 a          360 c          291 g          182 t
ORIGIN      
        1 ttcgccccgg cgaccgtgtc gccgcgctct ctcaaaacgc atttgctgaa tacgattact
       61 gcgccgcggc cgaggtcgcg ccgcttccgg cttcgctcgc cacgaagcca ttcccaggtg
      121 aagcgatcgg ctgcgcgatg aatgtctttc ggcgcagcga catccggccg catcatcacg
      181 tcgcgattgt cggactcggt tttctcggag cgctgctcac gcaactcgcc gcccgcacgg
      241 gcgcccgcgt ctatgcgatt tcgcgccgcc ctttcgccca ggaggtcgcg aagaagatgg
      301 gcgcgaccgc gacgttcggc atgagcgaca cctgggccac gatcgaagaa atcaagcagc
      361 tcactgaacg tgccggctgc gaacgcgtca tcgaagccac ggggctccaa gcgccgctcg
      421 atctcgccac ggaactcacc gccgaacgcg gcaaactcat catcgcgggc taccatcagg
      481 acggcctgcg tcaggttaac atgcagctct ggaactggcg cgggctcgac gtgatcaacg
      541 cccacgagcg cgatccacgc atttatcttc aaggtatgca ggaggccgcc gacgccgtcg
      601 cgtcgggtcg cctcgatccc acgccgctct acacgcaccg ctactcgttt gacgaacttc
      661 cgcgcgcctt gaaccagatg cgcgatcgta gcgacgggtt tctgaaagca ctcgtcacct
      721 atgaatgatt tcgccgtagc agcccgcgtc tccgcgtgcc gctcctttga actccatcct
      781 tctgctcggc tgacccagcg gcttgccgct acgtccatgg aatcatgacg ctgcaacccg
      841 aaccgcttct caccgtcgaa acgccgctgc ctcgcacgcc gcggctcggt tttgccggcg
      901 tcggctggat cgggcgcaac cgcttgcagg cgatcgccac cagcgggctc gctgaaattg
      961 ccgccatcgc cgattcggcc gcgggagcca tcgcggaaac gcgaccgtt
//
LOCUS       DASPLT010000221         1210 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_227812, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000221 DASPLT010000000
VERSION     DASPLT010000221.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1210)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1210)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1210
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_227812"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1023)
                     /locus_tag="VEA63_02350"
     CDS             complement(<1..1023)
                     /locus_tag="VEA63_02350"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HYD82853.1"
                     /translation="MKASPYYSSDDPALRRLARIALLAGAFAWPAAFVHGQAAPAEPT
                     ADDEETPIVLSPFVVDSTQDRGYAATSTLAGTRIRTDLADIGSSISVITREMLTDLGA
                     TNNESVLAYAVNTEIGGARGNFSGNIGVSGERYQEEGTFSNPNGNTRVRGLTSADNTR
                     NFFLTDIPWDSYNTSRVDLQRGPNAILFGLGSPAGVVNATTTSAEFRDRGRFEIAFDK
                     FSSQRYVLDVNQQIIPQQLALRVALLRDDKKFRQDPAFQLDQRAYAAITFRPEQLNRN
                     GNTFEVTANYEHGDISSNRPRVVAPIDRLTGYWDPLERGGVGKQVFDPYTTDININTH
                     ANLGGAG"
BASE COUNT          216 a          329 c          429 g          236 t
ORIGIN      
        1 accggcgccg ccgagattgg cgtgcgtgtt gatgttgatg tccgtggtgt agggatcgaa
       61 cacctgcttg ccgacgccgc ctcgttccag cggatcccaa taaccggtca gccggtcgat
      121 gggggcgacg acgcgcgggc gattcgagga gatgtcgcca tgctcgtagt tggcggtgac
      181 ctcgaaggtg ttgccgttgc gattgagctg ctcggggcga aacgtgatcg cggcgtaagc
      241 ccgctgatcc agttgaaacg ccggatcctg gcggaacttt ttgtcgtcgc gcaggagcgc
      301 cacgcgaagc gcgagctgct gcgggatgat ctgctgattg acgtcgagaa cgtagcgctg
      361 gctgctgaac ttatcgaaag cgatttcgaa tcttccacga tcgcggaact cggcgctcgt
      421 cgtggtggcg ttgaccacgc ccgctggact gccgaggccg aacaggatcg cgttcgggcc
      481 gcgctggaga tcgacgcgag aggtgttgta gctgtcccac ggaatatcgg tgaggaaaaa
      541 attacgcgta ttgtcggcgg aggtgaggcc gcgaacgcgc gtgttgccgt tgggattgga
      601 gaacgttcct tcctcctgat agcgctcgcc gctgacgccg atgttaccag agaagttgcc
      661 gcgggcgccg ccgatttcgg tgttaaccgc atacgcgagc acggactcgt tgttggttgc
      721 gccgagatcg gtcaacatct cgcgcgtgat gacggagatt gaagacccga tatcggcgag
      781 gtcggtgcgg atgcgcgtgc cggcgagcgt gctcgtcgcg gcgtaaccgc gatcctgcgt
      841 ggaatcgacg acgaacgggc tgaggacaat cggcgtttct tcgtcgtcag cggtcggttc
      901 cgcgggagcg gcctggccat gaacgaacgc cgcaggccag gcaaacgcgc cggcgagcaa
      961 agcgattcgc gcgagcctgc gcagagcagg atcgtccgag gagtagtaag gggacgcttt
     1021 catttgggtg tgggtaggat tgcttggttc gatgctgcac acaccagttc cgactcgcgg
     1081 acatgcggtt gcacccgacg ctccgcgaac gaccgctcgt tcgaggacac gaccggcttg
     1141 gtcaagcgcg gcggagcaat actccgattg ggtgggtttc acgttcgagg gttgggggaa
     1201 ccgatgaccg
//
LOCUS       DASPLT010000222         3843 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_25005, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000222 DASPLT010000000
VERSION     DASPLT010000222.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 3843)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3843)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3843
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_25005"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1257
                     /locus_tag="VEA63_02355"
     CDS             <1..1257
                     /locus_tag="VEA63_02355"
                     /inference="COORDINATES: protein motif:HMM:NF015803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ShlB/FhaC/HecB family hemolysin
                     secretion/activation protein"
                     /protein_id="HYD82854.1"
                     /translation="VAPAKLDGDLQIDGAKYFSEASYRAAVPLAAGSEIDASVVQAGV
                     ERLNRGGYRRVVIAAEPGATSGTTKLVLRTLERRPWSLSAGYNNTGTAVTDEDRVTAG
                     VVWGNAFDRGDTLGYSFSADPALEHSISHSANYGTVFKSGHSFTAFGSHSTIESALPA
                     PLTQEGKSWQTGLRYGVPLAKTAGGWERSFNVAADFKYSDNNLEFAAIPVTNNVTHIA
                     QLGATFSASRRTPTQGLALSASLYASPGGLTSENDDAAFERSRFGAKAAYVYGRFEGQ
                     YSRALPAGFSLSTTASLQLASGPLLGTEQVNGAGSAGVRGYRESSAFSDEGVVVNTEL
                     HLPAFAPFKGRGQADLFAFVDAAALWDLGPGATSAELASSGVGVNYRFGRVFTLSAAY
                     GWQWKEIPNIADQGAGHGHISAGLTF"
     gene            complement(1462..2394)
                     /locus_tag="VEA63_02360"
     CDS             complement(1462..2394)
                     /locus_tag="VEA63_02360"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HYD82855.1"
                     /translation="MKTVLLVDDNPANLGVLIDVLRGAGYRLLVAEDGERALHQMKNV
                     RADIVLLDVTMPGIDGFETCRRLKAEPAWTDIPVIFTTARDDVVDKVAGFAAGGVDYM
                     TKPLQPEEVVARVRTHLRLRELQSELEEKNAELAERNERLAAAAKLRRAAEQALADSL
                     DRAVVVAKRDGEILFCTKRAAHLLAQYFPAHGSERLPPALHGGGNGAPLRITPLIAHE
                     GDVFQILQLDPARPDPSPRDLEALGLTPREAEVLFWISEGKTNPEIAVILDTATNTVK
                     RHSQAVFDKLGVENRTVAARIAMETLRRTSVTPG"
     gene            complement(2411..3055)
                     /locus_tag="VEA63_02365"
     CDS             complement(2411..3055)
                     /locus_tag="VEA63_02365"
                     /inference="COORDINATES: protein motif:HMM:NF013921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HYD82856.1"
                     /translation="MKPLPSVGASLADARPTRRTDALHYGRVSLPAVRYFATFVTHER
                     KPWLPPAVAGMTCSALDVLRAWHAEGDRAVLAATIMPDHVHVLFQLGTRLTVGQCVGR
                     SKSVARKNAGYEDGWQRDFWEHRLRADESAEDYALYIFLNPIVPACCRSRGLGRGGGH
                     LMNRSTRSRACLDPREHRRPNGLAGRKNGFGISRCMSDEIGAGERRPYMTLSTP"
     gene            complement(3052..>3843)
                     /locus_tag="VEA63_02370"
     CDS             complement(3052..>3843)
                     /locus_tag="VEA63_02370"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HYD82857.1"
                     /translation="GELQLESSAGRGSRFWFAVTLPEIAPPSIAHERTRIITGYRGPR
                     RRVLVVDDEAANRDLLRDLLGGLGFETTATGEAAVALAACADGVTEAVLLDLRMPGQD
                     GFALAPRLRAAGRRPLAIVAMSASVYPVDREQAIGAGCDEFVPKPIRETELFAVLGRL
                     LGLEWTHADGAAPTRDSQTPFDLLPLPELAALDELLALAECGDVLKLTEILKSLRVAD
                     PKLAPFVSRIDALAASYQMRALSDALKKARKVAAGAGERRPYMHP"
BASE COUNT          676 a         1274 c         1240 g          653 t
ORIGIN      
        1 gtcgccccgg caaaactcga tggcgacctc cagatcgacg gcgcaaaata tttctctgag
       61 gcctcctacc gcgccgccgt gccgctcgct gcgggcagcg agatcgacgc ctcggtcgtg
      121 caggccggcg tggagcggct gaaccgcggc gggtatcgtc gcgtggtcat cgccgccgaa
      181 cccggtgcga cgtccggcac gaccaagctc gtccttcgca cgctggagcg ccggccctgg
      241 agcctcagcg ccggttacaa caacaccggc accgccgtga ccgacgaaga tcgtgtcacc
      301 gccggcgtcg tgtggggtaa cgccttcgat cgcggcgaca cgctgggcta tagtttttcg
      361 gccgatccgg ctctggagca ctcgatcagc cacagcgcca actacggcac ggtgttcaaa
      421 tccggccact cgttcacggc cttcggttcc cattcgacga tcgagtccgc cctgcccgct
      481 ccgctcacgc aagaaggtaa atcatggcag accggcctgc gttacggcgt tccgctggcg
      541 aagaccgcgg gcggttggga acgcagtttc aacgtcgccg ccgatttcaa atactccgac
      601 aacaacctcg agttcgccgc catcccggtc accaacaacg tcacgcacat cgcccaactc
      661 ggcgcgacgt tctcggcttc gcgtcgcacg ccgacgcagg gcctcgcgtt gtccgcttcg
      721 ctttacgcga gtccgggcgg gctcacctcc gagaacgacg acgcggcttt cgaacgctca
      781 cgcttcggag caaaggcggc gtatgtttac ggccggttcg agggtcagta ttcgcgcgcg
      841 ctccccgccg gtttctcgct gagcaccacg gcgagcctgc aactcgccag cggcccgctg
      901 ctcggcaccg agcaggtgaa cggcgccggc agcgccgggg tgcgcggcta ccgcgaaagc
      961 tccgccttca gcgacgaggg cgtggtggtg aacacggagc tgcacctgcc ggcgttcgcg
     1021 cctttcaaag gccgcggcca agctgacctg ttcgcgttcg tcgacgcggc ggcgctgtgg
     1081 gatctcggcc ccggtgcgac gagcgccgag ctcgcctctt ccggcgtcgg cgtgaactac
     1141 cgcttcggtc gcgtgttcac gctgagcgcc gcctacggct ggcaatggaa agaaatcccc
     1201 aacatcgcgg accaaggcgc gggccatgga cacatcagcg cagggctcac gttttagccg
     1261 ggaccaatcg gcaggaggcc accaggtgct tggaggcttt catgatggcg aacggcgaac
     1321 aacgaagcgt cgcacgtgca acctgtagtt ttgccgtgga ggtttgtagt ggaactctgg
     1381 ttacggacta gccgcccggg cgttgacctc ggagcgtgag cgccgcgaac aaaggtcggc
     1441 gctgccccac gccgagtcac ctcagcccgg cgtcacgctg gttcgacgca aggtctccat
     1501 cgcgatgcgc gcggcaacgg tgcggttctc cacgccgagt ttgtcgaaca ccgcttgaga
     1561 atggcgcttc acggtgttcg tggcggtgtc gaggatcacc gcgatctcgg gattcgtttt
     1621 gccttcgctg atccagaaaa gcacctcggc ttcgcgcggc gtcagcccga gcgcttcgag
     1681 atcgcgcggc gaaggatcgg ggcgcgcggg atcgagttgg aggatttgaa acacatcgcc
     1741 ttcgtgagcg atgagcggcg tgatccgtaa cggcgctccg ttgccgccgc cgtgcagcgc
     1801 gggtggcagg cgctcgctgc cgtgcgcagg aaaatactgc gcgagcaggt gcgcggcgcg
     1861 tttcgtgcaa aacaagattt cgccatcgcg tttcgccacg accacggccc gatcgagtga
     1921 atccgccaac gcctgctcgg cggcgcgacg caatttcgcc gcggcggcga gccgctcatt
     1981 tcgctcggcg agctccgcgt ttttttcttc cagctctgac tgcagctcgc gcagccgcag
     2041 gtgcgtgcgc acgcgcgcga cgacttcctc cggctgcaac ggcttggtca tgtagtccac
     2101 gccaccggcg gcgaatccgg cgactttgtc cacgacgtcg tcgcgcgccg tcgtgaagat
     2161 caccggaatg tccgtccacg ccggctctgc tttcagccga cgacacgtct cgaagccgtc
     2221 gatgcccggc atcgtcacgt cgagcaggac aatatcggcg cggacgtttt tcatctggtg
     2281 cagcgcacgc tcaccgtcct cggcgacaag caatcgataa ccggcgccgc gcaacacatc
     2341 gatgagcacg ccgagattgg caggattatc gtcgacgagc agaacggttt tcatgagcac
     2401 ggacctcagg ctaaggggtg gaaagtgtca tgtaggggcg ccgctcgccg gcgccgattt
     2461 catcactcat gcaccgagag atgccgaaac cgttcttccg gccagccaat ccattcggcc
     2521 ggcggtgttc cctcggatcc aagcaggctc gtgaacgtgt agatcgattc atcaggtgtc
     2581 caccaccacg gccaagtcct cgcgatcggc aacaagccgg cacgatagga ttcaggaaaa
     2641 tatacagcgc atagtcttcg gcggattcat cagcacgcag gcggtgttcc cagaagtcgc
     2701 gttgccagcc atcctcgtag cccgcgttct tgcgcgcgac cgatttcgag cgacccacgc
     2761 attgtcccac cgtgagtctc gtcccaagtt ggaacagcac atgcacgtgg tcgggcatga
     2821 tcgtcgcggc cagcacagct ctgtcgcctt ccgcgtgcca ggcgcgtaac acatcgagcg
     2881 cggagcaggt cataccggcg acggcggggg gtaaccaggg tttgcgttcg tgcgtaacga
     2941 aggtcgcgaa ataacggacc gcgggcaacg agacgcgtcc gtagtgaagc gcatcggtgc
     3001 gacgcgtggg tcgggcgtcg gcaagcgacg cccctacgga gggcaagggt ttcatggatg
     3061 catgtagggg cgccgctcgc cggcgcccgc cgcgaccttg cgcgcttttt tcaacgcatc
     3121 gctcaacgcg cgcatttgat aactcgcggc gagcgcgtcg atccggctga caaacggcgc
     3181 aagcttcgga tcggccacgc ggagcgactt cagaatctcc gtgagcttga gcacgtcgcc
     3241 acactccgcg agcgcgagca actcgtcgag cgcggcgagc tcaggcagcg gaaggagatc
     3301 gaacggcgtc tgcgaatcgc gcgtcggcgc agcgccgtcc gcgtgcgtcc actcgagacc
     3361 gagcagtcgg ccgaggacgg caaacagctc agtctcgcga atcggcttgg gcacaaattc
     3421 gtcgcagccg gcgccgatcg cttgctcgcg atccaccggg taaacgctcg cggacatcgc
     3481 gacgatcgcc agcggcctgc gcccggcagc gcgaagacgc ggtgcgagcg cgaagccatc
     3541 ctgacccggc atccgcagat cgagcagcac ggcttcggtt acaccgtcgg cacacgcggc
     3601 gagcgcaacg gcggcttcgc cggtggcagt cgtttcgaag ccgagaccgc cgagcaggtc
     3661 gcgcaacaag tcgcggttcg ccgcttcgtc gtcgaccacg agcacgcgtc gccgcgggcc
     3721 tcgatagccc gtgatgatgc gagtgcgttc gtgcgcaatc gagggtggtg cgatttcggg
     3781 cagcgtgacg gcgaaccaaa agcggctgcc gcgacccgcg ctactttcga gctggagttc
     3841 gcc
//
LOCUS       DASPLT010000223         1466 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_166790, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000223 DASPLT010000000
VERSION     DASPLT010000223.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1466)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1466)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1466
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_166790"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1334)
                     /locus_tag="VEA63_02375"
     CDS             complement(<1..1334)
                     /locus_tag="VEA63_02375"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HYD82858.1"
                     /translation="MIRRGGHWCYLAATAAVIAAPAAAQEAPDPFELSPEQLFGAEVM
                     SASRSPQSVWEAPAAIYVIDASDIERSGATTIAEALRLVPGVQVARTNTSGWAVSIRG
                     MNSALANKLLVLVDGREAYDPLFSGVYWDVQDTALQDIERIEVIRGPGAALWGANAVN
                     GVINIITRRAVDTQGALVSVVAGDTERASLTARFGSSAGERAHWRVYGRAFDRAGQDT
                     LAGADDNSSWSAWRGGFRTDIEIDADNALTVQGDLYHSETGQMRLVPVLAAPYASLEE
                     ESIVAEGANVLARWTRELDNDARLTAQAYIDITRRSQALLEDRRTTFDLDVQYDLPTL
                     GAHDFIAGVRYRNSADLVTQTEIITSPGRTHRSELLSAFIQDEISLSPRWRLTLGSKF
                     DNSDYSGFEVQPSARLQWMGDEHMAWASLSRAVRSPSELEREFDIVLGAGEIS"
BASE COUNT          271 a          453 c          454 g          288 t
ORIGIN      
        1 gagatttcgc ccgcgcccag tacgatgtcg aactcacgct ccaattcgct cggcgagcgc
       61 acggcgcgcg acagcgaggc ccaggccata tgttcatcgc ccatccactg caggcgtgcg
      121 ctcggttgca cctcgaaacc ggaataatcg ctgttgtcga atttcgaccc gagcgtcagg
      181 cgccatcgcg gcgagagcga aatctcgtcc tgaatgaacg cgctcaacaa ttcagaacgg
      241 tgcgttcgac ccggtgaggt tatgatctcg gtttgcgtca ccaagtcggc tgaattgcga
      301 tagcgcaccc cggcgatgaa atcatgagcg ccgagggtcg gcagatcgta ctgaacgtct
      361 aaatcgaagg ttgtgcgccg atcttcgagc agcgcctggc tgcggcgcgt tatgtcgata
      421 taggcttgcg ctgtcagccg tgcatcgttg tccagctcgc gcgtccagcg ggccagcaca
      481 tttgcgcctt cggcgacaat gctctcttct tcgagcgagg cgtaaggcgc cgccagcacg
      541 gggacgagcc gcatctgtcc ggtctcggaa tgatagaggt cgccctgcac cgtgagcgca
      601 ttgtccgcat cgatctcgat gtcggtccgg aagccgccgc gccaagcgga ccatgagctg
      661 ttatcgtcgg cgccggccag ggtgtcttgc ccggcgcggt cgaaggcgcg gccatagacg
      721 cgccagtgcg cgcgctcgcc cgcacttgat ccaaagcgcg cggttaagct ggcgcgttcg
      781 gtgtctcctg cgacgacgct gaccagcgcg ccctgggtat cgacggcccg gcgcgtgatg
      841 atgttgatga cgccgttgac cgcgttcgcg ccccatagcg ccgcacctgg accgcggatc
      901 acttcaatgc gctcgatgtc ctggagtgct gtatcctgca cgtcccaata aacgccggag
      961 aagagcggat cgtaggcctc tcttccatcg acaagtacga gcagtttgtt tgcgagcgcc
     1021 gaattcatac ctcggatgct gaccgcccaa cccgacgtat tggtgcgcgc gacctgcacg
     1081 cccggcacga gacgcaaggc ctccgcgatc gtggtcgcac cggagcgttc aatgtcggaa
     1141 gcatcgatga catagatcgc ggcgggcgct tcccacacgc tttgaggcga acgcgaggct
     1201 gacatgacct cggcgccgaa caattgttcg ggagataatt cgaacggatc gggagcttct
     1261 tgagcggcgg cgggcgccgc gatcactgcc gcggtcgccg caagataaca ccaatgcccg
     1321 ccgcgacgaa tcattatccc agcctcgtct gtagcgtaca cttcaggtct gatgcgtaaa
     1381 ccaaaggttg ttgcgagccg aagcggcgcg gattgtgccg ttcattttat gatttatggc
     1441 tcttaacgtc tggcccgggg tttgtg
//
LOCUS       DASPLT010000224         5214 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_208131, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000224 DASPLT010000000
VERSION     DASPLT010000224.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 5214)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5214)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5214
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_208131"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..286
                     /locus_tag="VEA63_02380"
     CDS             <1..286
                     /locus_tag="VEA63_02380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HYD82859.1"
                     /translation="YASKGVTGVYAPGVAFDGPVFVGDIDAFDAATPALVLRSAQELR
                     VTGGDLLQTNGKPVLIGELVPSNFTAGTTSHGDLLLAQPNRATFQPEDLP"
     gene            complement(469..867)
                     /locus_tag="VEA63_02385"
     CDS             complement(469..867)
                     /locus_tag="VEA63_02385"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HYD82860.1"
                     /translation="MKIKLNSILVDHQDKALDFYTGVLGFKKSKDIPVGEFRWITVVS
                     PDGHPDVELALEPNANPAGKTFQEAMFGQGIPLTAFEVDDVDAEAARLKQAGVKFTMD
                     PTFAGPMKLAVFADTCGNLIQIYQPPQEPA"
     gene            complement(882..1337)
                     /locus_tag="VEA63_02390"
     CDS             complement(882..1337)
                     /locus_tag="VEA63_02390"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HYD82861.1"
                     /translation="MLHHELLRRLCLARDWLREDAEPRPSVTQVARRAGIAPHHFIRL
                     FRAVFGETPHQYRSLAQIERAKHLLILTDQTVTEVCMAVGFSSLGSFSTLFTRRVGVS
                     PSVFQRRHRAPAGQARQLPPELIPGCVSLMALAHSAKSNFQEASAALPR"
     gene            1631..2494
                     /locus_tag="VEA63_02395"
     CDS             1631..2494
                     /locus_tag="VEA63_02395"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HYD82862.1"
                     /translation="MNACDTILDTATGPFAIRDHGGAGRGLLLVHGTGHNLEVWEPLA
                     DRLRGDFRVVSFDLRGHGQTPLNSANAEEHWRDLAAIIDALRLKQPILVGHSSGGYAV
                     TAHAAAGGACAGLVVVDGFVPDPREAALRATGVVPREQLRTMFRYGWIATDEQKEAWG
                     RQVRAEAPGDWLNFGIDPDLVEAFTRRSFMRHGDGWLRRPTLEEIDQLSRLDARQAIL
                     PANDVYERLQMPASFVFARHGLYRDRRAEVECLARKIGAQFVELDAGHNLHMQQPAAL
                     GEAIRDLANRV"
     gene            2590..2916
                     /locus_tag="VEA63_02400"
     CDS             2590..2916
                     /locus_tag="VEA63_02400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HYD82863.1"
                     /translation="MQSLLALADPTRRRIVELLAVRERTAGELVREFDMSAPAISQHL
                     NVLREAGLVITRAEGQSRIQTLNPDGLDDLEAWLEKTRSVWSRRLDALERELRADDAK
                     ARKKSS"
     gene            2913..3464
                     /locus_tag="VEA63_02405"
     CDS             2913..3464
                     /locus_tag="VEA63_02405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HYD82864.1"
                     /translation="MNIDNHEHAKFTGPGEVRLVRLLPGPIERVWQYLVDPEKRARWF
                     AGGLLEQKPGGRAALTMRQASLAPDETPPEKYKQMHHSGVTIEGRVLRCEPPRLLVFT
                     FGGDDSEVTFELTTQNSQVLLVLTHRAKGEDVPDLGNFATGWHTHLALLVAELEGAPR
                     PPLWPTHARLEADYEKLRAAAQR"
     gene            3797..>5214
                     /locus_tag="VEA63_02410"
     CDS             3797..>5214
                     /locus_tag="VEA63_02410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="heparinase"
                     /protein_id="HYD82865.1"
                     /translation="MSLAEVEAALPSQPAGVGAPIDDRAAWQYASRWPSIQRQASDAR
                     KFLREPIPVIDDAVIEEGRRKQDRQIYEIPMRLRLTRLNAFVVAEGLDDKGEFLPAIE
                     KEITAILDEAAWIVGGALTSEIGTPKSTQVDLVTAPRAWTLATADYWLGDRLPSELRR
                     RLRERLEERVFAPYLEDAAAEKKRFFWLEKQTNNWGPVCHAGIVGAALAVVDSRDYRA
                     RIAHTALTRLDGYLNGWSVSKDGLCIEGVGYWDYGFGNYLLLAETLWRQSSGEINLYA
                     GERVRQMATTVRSLELSHGTYPSFADCAIYMRPNVALLWLIKERFGIRESGWPEAPDG
                     VDALYRPEWAFQDFAGRPDPKELAGFMFALHPQGDRLAGYGTFAFPLPPGIANQGAPV
                     VMDPRRSIFIDSGIAVLRDVAPGKPVLTLAVKGGHNDEPHGHHDLGSYVIAVDGYPLL
                     LDPGAELYTPSTFSPRRFESMM"
BASE COUNT          915 a         1552 c         1744 g         1003 t
ORIGIN      
        1 ttatgcgagc aagggcgtga ccggagttta cgcgcccggc gtggcgttcg acgggccggt
       61 tttcgtcggc gatatcgacg ccttcgatgc cgccacgcct gcgctcgtgc ttcgcagcgc
      121 gcaggagctg cgcgtcactg gcggcgattt gctccaaacg aacggcaaac ccgtgctcat
      181 cggcgagctg gtgcctagca atttcaccgc cggcaccacg tcgcatggcg atctcctcct
      241 cgcgcagccg aaccgcgcca ccttccagcc tgaagatttg ccgtgaggcc gaaggtcggg
      301 gtccggaaga aacacggacg gtttccggcg cgcatcgaaa aatgcctgcc gcagattttc
      361 gcggggaagg gattgaatga cgagcggcgg tcccggtagt ccggccgagt cgccgatcgc
      421 ttcagccggt gattcgcggc gaggcggacg ctcgaccgtc cttcgggttc aagccggctc
      481 ctgcgggggc tgatagatct ggatcaagtt gccgcaggtg tcggcgaaca cagcgagttt
      541 catcggcccg gcaaacgtag ggtccatcgt gaacttcacg cccgcttgct tcagtcgtgc
      601 ggcttcggcg tcgacgtcgt ccacttcgaa cgcggtgagc gggatccctt gtccaaacat
      661 cgcctcctgg aaggttttgc cggcgggatt tgcattgggt tcgagcgcga gttcgacgtc
      721 agggtgcccg tcgggagaga cgaccgtgat ccagcgaaat tcgccgacgg ggatgtcctt
      781 gcttttcttg aagccgagga cgccggtgta gaagtcgagg gccttgtcct gatggtcgac
      841 gaggatgctg ttcagtttga ttttcatacg tggggacgag cttaccgcgg aagcgcggcg
      901 gacgcttctt gaaaattgct ttttgccgaa tgagcgaggg ccatgagaga gacgcagccc
      961 gggatcagtt ccggcggcag ctggcgggcc tgaccggccg gcgcacggtg ccgtcgctga
     1021 aacaccgacg gcgacacgcc cacgcggcgc gtgaagagcg tgctgaagct gccgagactg
     1081 gaaaatccga ccgccatgca cacctccgtg accgtttggt cggtcagaat cagcaggtgt
     1141 ttcgcgcgct cgatttgcgc gagcgagcga tactgatgcg gtgtctcgcc aaagactgcg
     1201 cggaagaggc ggatgaagtg atgcggcgca atcccggcgc gccgcgcgac ctgggtcact
     1261 gacggccgcg gctccgcgtc ctcgcgcagc caatcgcgag caagacagag gcgacgcagg
     1321 agttcgtggt gcagcatgaa gggatgagcg gccgagccga tgttgtggtg tttcgagcca
     1381 actcaccgct ggtcgcaaaa gggcgcagaa aacgtaaaga gatcatttca tgccgcggag
     1441 gccagtcttc ctcgcggaaa acagctcacc gagctgaact tgcgccatcg tgcttttttc
     1501 agaatagccg tgctacgtct gtggcgctcc gcgaatccgc agtgttggtc gggatttctc
     1561 ctcacgacgt tgcgcgacag ggactcgtgc ggatgatagg tgtccgtcgc tttttgaggc
     1621 ggtttcgccc atgaacgcct gtgacacgat tttggatacg gcgaccggtc cgttcgcgat
     1681 ccgcgatcac ggcggcgcgg ggcgcggcct tttgctggtt cacggcacgg gccataatct
     1741 cgaagtttgg gaaccgctcg cagaccggtt gcgcggcgat tttagggtgg tgagcttcga
     1801 tctgcgcgga cacggacaaa cgccgctcaa ttccgcgaac gccgaagaac attggcgcga
     1861 tctggccgca atcatcgacg cgttgcggtt gaaacagccg attctggtcg ggcactccag
     1921 cggcggttat gccgtcaccg cgcatgcggc ggcaggaggg gcgtgcgcgg ggttggtggt
     1981 ggtggatggc tttgtgcccg acccacgcga agcggcgctg cgcgcaaccg gcgtggtgcc
     2041 gcgcgagcaa ctgcggacga tgtttcgcta tggctggatc gctacggacg agcagaagga
     2101 agcttggggg aggcaggtgc gcgccgaggc gccgggcgat tggctgaatt tcggaatcga
     2161 tccggatttg gtcgaggcct tcacccgccg ctcgttcatg cgccacggcg atggctggct
     2221 gcggcggccg acgttggagg agattgatca actcagccga ctcgatgcgc ggcaggcgat
     2281 cctgccggcg aatgacgttt acgaacgctt gcagatgccc gcgagttttg tcttcgcccg
     2341 gcacggcctt tatcgcgatc gacgcgccga agtggagtgc ctcgcgagaa aaataggtgc
     2401 gcaattcgtc gagctcgacg ccggtcacaa cctgcacatg cagcaacccg ccgcgctcgg
     2461 cgaagcgatc cgcgacctgg cgaaccgcgt atgaacaccc aagcatttgg cccagaccgg
     2521 agccgtcgtt cgatcgcacc gttcgtttta aatcttgctt aattaagtta atgcttaatc
     2581 cgtgtccgca tgcaaagtct cctcgctctc gccgatccga cacggcggcg gattgtagaa
     2641 ctgctcgcgg tgcgcgagcg aaccgcgggc gagctcgtca gggaattcga catgagcgcg
     2701 ccggcgattt cgcagcacct caacgtcctg cgcgaagcgg gactcgtcat tacccgcgct
     2761 gaaggccagt cgcgtatcca aacgctcaat cccgacggtc tcgatgatct cgaagcgtgg
     2821 ctggagaaga cgcggtcggt ctggtcgcgc cggctcgatg cgttggagcg cgaactgcgg
     2881 gccgacgacg ccaaggcgcg gaaaaaatca tcatgaacat cgacaaccac gaacacgcca
     2941 aatttaccgg cccgggagag gtgcgactcg tccgtttgct gcccggcccg atcgaacgcg
     3001 tttggcaata tctcgtcgat ccagagaagc gcgcgcgctg gtttgccggc ggcttgctcg
     3061 agcaaaagcc gggcggtcgc gccgccttga cgatgcggca ggcgagtctc gctcccgacg
     3121 aaacgccgcc ggagaaatac aagcagatgc accactcggg cgtgacgatc gagggccgcg
     3181 tgcttcgttg cgaaccgccg cggttgttgg ttttcacttt cggcggcgac gattccgagg
     3241 tgacctttga gctcaccacg cagaacagcc aggtgctgct ggtcctcacg catcgtgcga
     3301 agggcgagga tgtgcccgac ctcggcaact tcgcgaccgg ctggcacacg catctcgcgc
     3361 tgctcgtcgc ggagttggaa ggcgctccgc ggccgccgct ttggccgacg cacgcgcggc
     3421 ttgaagccga ctacgagaaa ctccgcgccg cagcgcagcg ctgagccgag ctcgggtcgt
     3481 agaaggtgga gcccggcgtc cccgtcgggc tgtttggctt cttccaccgg gcggatgcct
     3541 cggagaggca tccctaccag tgcgtccgac gtcgcgaaat taaaccgcta taagtcgtcc
     3601 gagttttgga ctgaggcgtg ccgttgcgct ttccggcctt caccgacgtt ctggctggtc
     3661 caactgcgcg tcgcgtcctg atcttgacgc ctgcgacgtg gttaccggta gtcacagcgt
     3721 gttacctacc tctcgtctgt ttctgtctgt cgtcttgggg ttcttcgcgg ctgcttctgt
     3781 tgcggctgag ccatcgttga gtttggccga ggtcgaagcg gcgcttcctt cgcaacccgc
     3841 gggtgtgggc gcgccaatcg acgatcgcgc ggcgtggcag tatgcgtcgc gctggccatc
     3901 gatccagcgt caggcgagcg atgcgcgaaa atttcttcgc gagccgatcc cggtgatcga
     3961 cgacgcggtc atcgaagaag gacgccggaa acaagatcgg cagatctacg aaatcccgat
     4021 gcgcctgcgg ctgacgcgcc tgaacgcgtt tgtcgtggcg gagggtttgg acgacaaggg
     4081 cgaatttctt cccgcgatcg aaaaggaaat cacggcgatt ttggacgagg cggcgtggat
     4141 cgtcggaggt gcgctcacgt cggagatcgg cacgccgaag agcactcagg tcgacctcgt
     4201 caccgcgccg cgggcgtgga cgctggcgac ggccgattac tggctcggcg accggctgcc
     4261 gtccgaactg cgccgccgtt tgcgcgagcg gctcgaggaa cgcgttttcg ctccttatct
     4321 cgaagacgcg gcggcggaga agaagcgttt tttctggctc gaaaagcaaa ccaacaactg
     4381 gggcccggtg tgtcacgccg gcattgtcgg cgccgcgctc gccgtcgtcg attcgcgcga
     4441 ctaccgcgca cggattgctc acacggcgct gacgcggctc gacggttatt tgaacggctg
     4501 gtcggtgtcg aaggacggac tctgcatcga gggcgtcggg tattgggatt atggattcgg
     4561 gaattatctg ctgctggccg agacgctctg gcggcagtca agcggcgaga tcaatttgta
     4621 tgccggcgaa cgcgtccgac agatggcgac gaccgtccgc agcctcgagc tcagtcatgg
     4681 gacgtatcca tcttttgcgg actgcgcgat ctacatgcgg ccgaacgtcg cgctgctttg
     4741 gttgatcaag gaacgtttcg gcatccgtga atcaggctgg ccggaggctc cggacggcgt
     4801 cgatgcgctg tatcgcccgg agtgggcctt tcaggatttt gcgggccggc cggacccgaa
     4861 ggaactggcg ggcttcatgt ttgctttgca cccgcagggc gaccggctcg cgggatatgg
     4921 gacctttgcg tttccgttgc caccggggat cgccaaccaa ggcgcaccgg tggtgatgga
     4981 tccgcggcgt tcgatcttca tcgactccgg aatcgcggtg ctgcgcgatg tcgcgccggg
     5041 gaagccggtg ctcacgctcg cggtgaaagg cggccacaac gacgagccgc acggccatca
     5101 cgatctcggc agctatgtga tcgcggtcga cggttatccg ctcctgctcg atcccggagc
     5161 ggagctttat acaccgtcga cgttcagtcc gcgacggttc gagagcatga tgaa
//
LOCUS       DASPLT010000225         1955 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_84778, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000225 DASPLT010000000
VERSION     DASPLT010000225.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1955)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1955)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1955
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_84778"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..448
                     /locus_tag="VEA63_02415"
     CDS             <1..448
                     /locus_tag="VEA63_02415"
                     /inference="COORDINATES: protein motif:HMM:NF014448.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein MoaE"
                     /protein_id="HYD82866.1"
                     /translation="RARPIAMFKISAEPLDPAALKRDLLDAHAGACVTFEGWVRDHND
                     GRPVRALDYEAFASLAEREGERILAEAHEKFSIVALRCVHRVGALQIGDLAVWVGVAA
                     AHRGAAFDACRYVIDETKARVPIWKKEHYADGSNEWVNCATRSAAL"
     gene            864..1235
                     /locus_tag="VEA63_02420"
     CDS             864..1235
                     /locus_tag="VEA63_02420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007363710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HYD82867.1"
                     /translation="MTAHPYAFLALFLAVALAFPLIMLAVAKTFARVFQRAKPGPHKN
                     ATYECGLPSTGDSWIQFKAHYYLYAIFFLIFDVEVLFLLPFAVAFTGLAPGALLAMLV
                     FLLLLAEGLVWAWHRGYLEWK"
     gene            1232..1891
                     /locus_tag="VEA63_02425"
     CDS             1232..1891
                     /locus_tag="VEA63_02425"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009509700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B"
                     /protein_id="HYD82868.1"
                     /translation="MSDANTNATAPISDAERDDLRRHGILLTSLEELHNWGRSNSIWP
                     LQFGLACCAIEMIATATARFDIARFGAEIFRPSPRQADLMIVSGTVTKKMAPQVVRLW
                     NQMPEPKYCIAMGACAISGGPFKQGYNVLKGIDRFIPVDIYIPGCPPRPEALLNGLIE
                     LQKKIRAERLTGPDAARHTQADKPSEYPIPEFGEHDLVPPKNPDVWQPPAPARSSKPQ
                     A"
BASE COUNT          380 a          699 c          527 g          349 t
ORIGIN      
        1 acgcgctcga ccgatcgcca tgttcaaaat ctccgccgaa ccgctcgacc ccgccgcgct
       61 caaacgcgac ctgctcgacg cccacgccgg cgcgtgcgtg accttcgaag gctgggtgcg
      121 cgatcacaac gacggccgcc ccgtgcgcgc gctcgattac gaagcgttcg cctcgctcgc
      181 cgaacgcgag ggcgagcgca tcctcgccga agcgcacgag aaattttcca tcgtcgcgct
      241 ccgctgcgtg catcgcgtcg gtgcgttgca gatcggcgat ctcgccgtgt gggtcggcgt
      301 cgccgccgcg caccgcggtg cggcgttcga cgcctgccgc tacgtgatcg acgaaacgaa
      361 ggcgcgcgtg ccgatctgga aaaaagagca ctacgccgac ggctccaacg agtgggtcaa
      421 ctgcgcgacg cgcagcgccg ccctttagta tgctgtccta caagaaacgt gaataaatcg
      481 aggggcgcgg cagcgccggt agggacgcct ctccgaggcg tccgcgtaaa cggaaacgca
      541 gcgactttcg aaaagcatct ctgggacgtc tctttgagcg gttcaaaaaa ttctatagcc
      601 aagccttggc cgcaaagacg cgcagaaggc gcaagaactc atgcgcccca agtcattctt
      661 tgcgccttct gtgcgttttt gcggctatag ttttgattga tctactttaa ccgtcgcaac
      721 tccgcgcccc aaagcggagc agcgagccca cgcacccgct cccaactcca gcgacgccaa
      781 tcgacgcgca ggtttgcgca aaaaagagac gcgcttcgac gttgagcttt gaaatcggca
      841 tttcatcgtt tcgttgcgca tccatgacgg ctcaccccta cgcgttcctg gccctgttcc
      901 tcgccgtggc gctcgcgttt ccgctgatca tgctcgccgt cgcgaaaacg ttcgcgcgcg
      961 ttttccagcg cgcgaaacct ggcccgcata aaaacgccac ctacgaatgc ggcctgccct
     1021 ccaccggcga ttcgtggatt cagttcaaag cacactacta cctctatgcg attttctttc
     1081 tgatcttcga cgtggaagtc cttttcctgc tgccgttcgc cgtcgccttc accggcctcg
     1141 cccccggcgc attactcgcg atgctcgtct tcctcctgct gctcgccgaa ggcctcgtct
     1201 gggcctggca ccgcggctac ctcgaatgga aatgagcgac gcaaacacaa acgccaccgc
     1261 tccgatcagc gacgccgagc gcgacgacct tcgccgccac ggcatcctcc tcaccagtct
     1321 cgaggagctg cacaactggg gccgcagcaa ctccatctgg ccgctccagt tcggcctcgc
     1381 ctgctgcgcg atcgagatga tcgcgaccgc caccgcgcgc ttcgacatcg cccgcttcgg
     1441 cgcggaaatc tttcgcccgt caccgcgcca ggctgacctc atgatcgtct ccggcaccgt
     1501 caccaagaaa atggcgccgc aagtcgtccg cctctggaac cagatgcccg agccgaaata
     1561 ctgcatcgcc atgggcgcgt gtgcgatttc gggcggtcct ttcaagcaag gctacaacgt
     1621 cctgaagggc atcgaccgct tcatccccgt cgacatctac atccccgggt gtccgccgcg
     1681 ccccgaagcc ttgctcaacg gcctgatcga gctgcagaaa aaaatccgcg ccgaacgcct
     1741 taccggcccc gacgccgcgc gtcacacgca ggccgataaa ccgagcgagt atccaattcc
     1801 tgaattcggc gaacacgatc tcgtcccgcc gaaaaatccc gatgtctggc agccgccggc
     1861 gccggcgcgc agctcgaaac cgcaggcgtg agacccaaag cccggcgccg gaagctccaa
     1921 actccaagca ccaagctcca atgaacctcc aacct
//
LOCUS       DASPLT010000226         1357 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_104627, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000226 DASPLT010000000
VERSION     DASPLT010000226.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1357)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1357)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1357
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_104627"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..471
                     /locus_tag="VEA63_02430"
     CDS             <1..471
                     /locus_tag="VEA63_02430"
                     /inference="COORDINATES: protein motif:HMM:NF016296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HYD82869.1"
                     /translation="DDARFAAALERAKHAVRQRVAESMDARLSQLLAYLETSGQAQPP
                     HGTDRILVKSSGEIFFLKAEEIDWIEAEGDYMKFHVAGRTHLMRETMARLEARLNPRQ
                     FIRIHRSTIVNVDRMRKLSPSFAGEYAVILQDGTKLKLSRGYHERIATLLKQSL"
     gene            716..>1357
                     /locus_tag="VEA63_02435"
     CDS             716..>1357
                     /locus_tag="VEA63_02435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD82870.1"
                     /translation="MTWPVWSMASTPLDHVRRARALLGTETWARIVQVENTNARSVYP
                     PTVYGLVFELGGLLWFYTDTDGTQSFSLHRNNLAVEKADFAPLLRDIEPGFANHSILE
                     ENAPSATPATGKEPLPNGCLIESYAALRERAASGELILRARLLSYYVRGRRAGHTVLA
                     YETPAGLHVIDPAVSERPRKVKQPSNDDPLVLARKIERGRAIAEARWLPTISAL"
BASE COUNT          241 a          371 c          490 g          255 t
ORIGIN      
        1 gacgatgcgc gcttcgcggc ggcgctggag cgggccaagc atgcggtgcg acagcgcgtg
       61 gcggagagca tggatgcgcg gttgagtcag ttgctcgcgt atctggagac gtcgggtcaa
      121 gcgcagccgc cgcacgggac ggatcgcatt ctcgtaaaat cgtcggggga gatttttttc
      181 ctgaaggccg aggagatcga ttggatcgag gcggagggcg actacatgaa atttcacgtc
      241 gcaggtcgca cccatctgat gcgggagacg atggcgcggc tggaggcgcg gttgaatccg
      301 cggcagttca tccggattca tcgatcgaca atcgtgaacg tcgaccgcat gcgaaaactg
      361 agcccgtcat tcgccggtga atacgcggtg atcctgcagg atgggacgaa gttgaaattg
      421 agccgcgggt atcacgagcg gatcgcgacg ctgttgaagc agtcgctgta agggcgccgc
      481 ggccagcggc actgcggcgc cgctgcccgg ctcacgaaaa gcttacgctt ttcgctacga
      541 aggcgtgggc ggcgcttcga aggcatgggc ggcgtatcca taaaaacgat tcggcgcggc
      601 ggcgaagacg ttcgccgcga agtgggcggg gttcgtcgca tttctgcaac gaccgggccg
      661 cgtgcacggt tttgtcgccg tgctccccgt gtttcgagtc gttttgcttt tgctgatgac
      721 ctggccggtc tggtcgatgg cgtctacgcc gctcgatcat gtgcggcgcg cgcgcgcttt
      781 gctcggcacg gagacgtggg cgcgcatcgt tcaggtggaa aacacgaatg cgcggagcgt
      841 ttatccgccg accgtttacg gactggtctt cgagctcggc gggttgctgt ggttctacac
      901 cgacacggat ggcacgcaga gtttctcgtt gcaccggaac aatctcgcgg tcgagaaggc
      961 ggactttgcg ccgttgctcc gcgacatcga acccggcttc gcgaatcact cgattctcga
     1021 agagaacgct ccgtcggcga cgccggcgac gggaaaggag ccgctgccga acggctgttt
     1081 gatcgaaagc tacgcggcgt tgcgcgagcg ggcggcgagc ggcgagctga tcctgcgggc
     1141 gcggttgttg tcgtattatg tgcgtgggcg gcgagccggg catacggtgc tcgcgtatga
     1201 aacgccggcg gggttgcacg tgatcgatcc ggcggtgagc gagcggccga gaaaagtgaa
     1261 acagccgtcg aacgacgatc cgttggtgct cgcgcggaaa atcgaacgag ggcgagccat
     1321 cgcggaggcg cggtggttgc caacgatctc ggcgctc
//
LOCUS       DASPLT010000227         1429 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_268944, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000227 DASPLT010000000
VERSION     DASPLT010000227.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1429)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1429)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1429
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_268944"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            322..771
                     /locus_tag="VEA63_02440"
     CDS             322..771
                     /locus_tag="VEA63_02440"
                     /inference="COORDINATES: protein motif:HMM:NF025701.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease complex subunit PrcB family protein"
                     /protein_id="HYD82871.1"
                     /translation="MLRLLFVLPLLLLGCHSQPSSSATPPTILAEWSGQHSGVNTAST
                     QIVRDAAAWSALWQQIGREPPHAFNPSRETAVAIFLGQRRTGGYRVEFAGLQREADQF
                     VLNYREEKPAPGAMVTQVISSPWAVAIISSVNQPIVTRQRPPATPPR"
     gene            1142..>1429
                     /locus_tag="VEA63_02445"
     CDS             1142..>1429
                     /locus_tag="VEA63_02445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtA"
                     /protein_id="HYD82872.1"
                     /translation="MSDTPTSRSRARRYFLYAVVALFLGWATWYFAFRAKPERNRYPQ
                     PAWAGTEYPLRVPVRTVAAKLVDLPVHLKAIGTVTPLNTVTVKSRVDGQLLR"
BASE COUNT          275 a          571 c          319 g          264 t
ORIGIN      
        1 ccgacaccga tcgctaaacc aaggcaccac ccaatccgcc aaaaaccggt cagtgctccg
       61 catttcatcg ccccacccaa cacccctcgc cctcgccgca ccaccccaaa ttccccaccc
      121 tcacccgccc agagaaccaa cgaaagtgtc acgcattgcg tgacactctc gaaaacgctt
      181 tcaccctttg gcccgcctcc cgttctccaa agtatcacgt aatacgtgac acttttcccg
      241 tgccacgtcg gtcgagcgct gccgattgcg accgcgccgc cgcgtgctac gctgcccatc
      301 agccctcgct cacgctctcc catgcttcgc ctcctcttcg ttctcccgct cctccttctc
      361 ggctgccact cccagccgtc atcctcggcc actccaccca cgatcctcgc cgagtggtct
      421 ggccaacaca gcggcgtgaa caccgcctcc acgcaaatcg tccgcgacgc cgcggcctgg
      481 tccgcgctct ggcagcaaat tggtcgcgaa ccaccgcacg ctttcaatcc ttcgcgcgag
      541 accgccgtgg ccattttcct cggacaacgc cgcaccggcg gctaccgcgt cgaattcgca
      601 ggactgcaac gcgaagcaga ccaattcgtc ctcaactacc gtgaagaaaa gcccgccccc
      661 ggcgcgatgg tcacccaagt gatctcctcg ccttgggccg tggccatcat ctccagtgtc
      721 aaccagccga tcgtcacccg ccagcgtccg cccgcgacac caccgcggta acttagatca
      781 atcccaccgc agcactcgcc acccgagttg aagagacaaa atcttcccgc ggtcttcccc
      841 aggaaagtag tgcggtgctt cagcccgcac ctcggctcac aacgcagtct gacgcgccgc
      901 ctacttacag catttatcaa cgaaaccgca gtcgctcaaa cgaagggccg gcctccgcgc
      961 cggcagcgaa cgacacagag gtcgtccctc caaaacagcc gcagcgttga aaacgctcta
     1021 cattcgcaat cccgccgccg aaaacccttt cacacactcg tttcccgact gtccgttacg
     1081 aatttctccg catttcggcc cggcgtgtag ccttccgcgt tcgcgttcgt catagcgggc
     1141 aatgtccgac actccgacgt cccgttcgcg cgcacgccgc tattttctct atgccgttgt
     1201 ggccctcttt cttggctggg ccacttggta tttcgccttc cgcgcgaaac cggagagaaa
     1261 ccgctatccc caacccgcct gggccggcac cgaatatccg cttcgcgttc ccgttcgcac
     1321 ggtcgccgcg aaactcgtgg acctccccgt ccacctgaaa gcgatcggca ccgtcactcc
     1381 gctcaacacc gtcaccgtca aaagccgcgt cgatggccaa ttgcttcgc
//
LOCUS       DASPLT010000228         1670 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_25149, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000228 DASPLT010000000
VERSION     DASPLT010000228.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1670)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1670)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1670
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_25149"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(551..>1670)
                     /locus_tag="VEA63_02450"
     CDS             complement(551..>1670)
                     /locus_tag="VEA63_02450"
                     /inference="COORDINATES: protein motif:HMM:TIGR01976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cysteine desulfurase-like protein"
                     /protein_id="HYD82873.1"
                     /translation="AISDYLCRSNSNTHGHFETSRRTDETIAAARSAMSDFLGCAPSE
                     VVFGPNMTTLTFAMSRAIGRDLSPGDEILITTLDHDANVAPWRALEERGCVIRQADVR
                     TADCTLDLDDLRAKITARTKLVAVGYASNAVGTINPVAEIVKIAHSAGALVFVDAVHY
                     APHGSIDVRALDCDFLACSVYKFFGPHVGVLYGKHEHLSRLRPYKVRPSSDAVPDRWE
                     TGTHNHECLAGVTAAVEYLASIGERLDPVQRTRRAKLLSAWTAIRHHEAELSEALITE
                     FTQIDGLTLYGIRDLAQLAKRTPTVAIRIDGHSPQELATHLGERGIFTWDGNYYALNL
                     TQRLGVESSGGMLRIGLAHYNTREEIESLVTALREIVR"
BASE COUNT          334 a          432 c          588 g          316 t
ORIGIN      
        1 ttcgcccccg cgctcgagtc cccggatgta cgcggcttga agaatgtcct cggcgacagc
       61 acgcgagccg acgcgacgtt cgatgaagga cagaaacttg ccacggtggt cgatgagttt
      121 cttaaggacg tcgctggaaa gagtctcggg cacgaaaatt tctcctgaac aactgtaaga
      181 atcgcgcaag gtcgttcgtc agcacagatg aacacggcgc cgaaggcgtc gcagaattta
      241 aggaggaatg aaatgactac cgaaaaaacg agttgttgtg gtgagtgtgt gtgcgagaag
      301 ccggcagctt cttgtgaatg cggaaccgaa tgcgagtgca agccgaagtg cgagtgcgga
      361 gagaactgcg gctgcgcgaa gtgaattggc cccttgccgt cccaccctgt tggtgaaggg
      421 aagcgacagg gtggggcagc gccgccctgt cgctcttcgt acctcgagcg gcctgcactc
      481 atgcggcgca tgaacggttg gtacaagggg ccgcaacgag cggccctttc catgtgagtc
      541 cgagtaaggt ttagcgcacg atttcgcgca gggcagtgac gagactctca atctcttcgc
      601 gagtgttgta gtgggcgaga ccgattcgca gcatcccacc gctgctttcc acgccgagac
      661 gctgtgtgag gttcagggcg tagtagtttc cgtcccaagt gaagatgccg cgctcgccga
      721 ggtgagtggc gagttcctgc ggagagtggc cgtcaatccg gatggcgaca gtgggcgttc
      781 gtttggcgag ttgagcgaga tcgcggatgc cgtaaagcgt cagcccatcg atctgcgtga
      841 actccgttat gagcgcttcc gagagttcgg cctcgtggtg gcggatggcg gtccaggcgg
      901 agagcagctt cgcgcgccgg gtccgctgga caggatccag tctttcgcct atggaagcga
      961 gatattcgac cgccgccgtg accccggcga gacactcatg attgtgcgtg ccggtctccc
     1021 agcggtcggg aacggcgtcg gaggatgggc gaactttata agggcgtagc cgcgagaggt
     1081 gctcgtgctt gccgtagaga acgccgacgt gcggaccgaa gaacttataa accgagcagg
     1141 cgagaaagtc gcaatcgagc gcgcgaacgt cgatggatcc gtggggcgcg tagtgaacgg
     1201 catccacgaa gaccagcgcg ccggcggagt gagcgatctt gacgatttcg gcaacaggat
     1261 tgatggttcc aaccgcgttc gacgcgtagc cgacggcgac tagctttgtc ctggcggtga
     1321 tcttggcgcg taagtcgtcg aggtcgagcg tgcagtcggc ggtgcgaacg tcggcctggc
     1381 ggatgacgca accgcgctcc tcgagtgcgc gccagggtgc gacattggcg tcgtggtcga
     1441 gggtggttat caggatctcg tcgccagggg aaagatcgcg acctatggcg cggctcatgg
     1501 caaaggtaag cgtcgtcatg ttggggccga aaacgacttc agacggcgcg caaccgagga
     1561 agtccgacat tgcggaacgg gccgcggcga tggtttcgtc agtgcggcgg ctggtttcga
     1621 agtggccgtg ggtattcgag ttggagcgac agaggtagtc ggagatggcg
//
LOCUS       DASPLT010000229         1954 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_43584, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000229 DASPLT010000000
VERSION     DASPLT010000229.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1954)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1954)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1954
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_43584"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1501)
                     /locus_tag="VEA63_02455"
     CDS             complement(<1..1501)
                     /locus_tag="VEA63_02455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 28-related protein"
                     /protein_id="HYD82874.1"
                     /translation="MLLLGAGCAALAMRAGDAVGANVTWTTYEAEAMKGTGEILGPRY
                     DPHRVETEASGQRCVRLGSSGEFVEFAAGADANAMVVRYSLRDATAGGGVESALSVFV
                     NGRAVRTLTLTSHYSWLYGNYPFSNRPGDEKPRNFYDEIRLKDLNIAAGDVVRVEKIG
                     PDAFPCIVDFVDLENVPPPLAAPADAFSVFDFGAVGDGQADDTAALRACIAAAERGGG
                     VVWVPPGEYKLTGDVVLTAKVAIQGAGMWHTTFVGDDALYAQSDRRVRFKLSGREIRL
                     ADFAIVGKLNYRNDSEPNDGIVGAGCAESFIERVWVEHTKAGVWVYNGTDLRIEGCRF
                     RNLIADGVNLCVGTSRSVIENCTARGTGDDCFAIWPAAPDQGFVGEGVPGNNIIRRCT
                     GQLTFLANGAAVYGGANNRVEDCLFTDISTGCGILISTTFPTTDEARRIDNNFTGTTV
                     IRNCELRRCGGYDHGWAWRGSLQICLDRRSIAGESAASRATPNRDRNRRS"
     gene            complement(1519..>1954)
                     /locus_tag="VEA63_02460"
     CDS             complement(1519..>1954)
                     /locus_tag="VEA63_02460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyl hydrolase family 65 protein"
                     /protein_id="HYD82875.1"
                     /translation="ENGSSLSACAYAMVAAEFGDVDFAYEYFLKTAKIDLEAKYKVYA
                     GTVFMGGTHPAANGGAWMTAVFGFGGVQVDEQGITIAPRLCRKWRKLEFNLDYRGDRL
                     RVSITKSAVTIAPAASNARAHRAVIAGRKVKCLPGKSVTVSV"
BASE COUNT          366 a          697 c          526 g          365 t
ORIGIN      
        1 cgctgcggcg gttacgatca cggttgggcg tggcgcggct cgctgcagat tcgcccgcga
       61 tgcttcgccg atctaggcaa atctgcagcg agccgcgcca cgcccaaccg tgatcgtaac
      121 cgccgcagcg ccgcagctcg cagtttcgaa tcaccgttgt cccggtgaaa ttgttgtcga
      181 tcctcctcgc ttcgtccgtg gtcggaaatg tcgtgctaat caggataccg cagccggtgg
      241 aaatgtcggt gaaaagacag tcctcgacgc gattgttcgc tccgccgtag accgccgctc
      301 cgtttgctag aaacgtgagc tgacccgtgc agcggcggat gatgttgttt cccggcacac
      361 cttcgccgac gaacccctga tccggagccg ccggccagat cgcgaaacag tcgtcccccg
      421 tgccgcgcgc ggtgcaattc tcgattacgc tgcggctcgt gcctacgcaa agattcacgc
      481 cgtcggcgat caggtttcga aaccgacaac cttcgatccg cagatccgtg ccgttgtaaa
      541 cccacacgcc cgccttggtg tgctcgaccc aaacgcgttc gatgaacgac tccgcacagc
      601 cggcgccgac gatcccgtca ttcggctcac tgtcattgcg gtagttcaac tttcccacga
      661 tcgcgaagtc cgcgagccgg atctcccgcc cgctgagctt gaaccgcacg cgccggtcag
      721 actgcgcata aagcgcgtca tcgccgacga aggtcgtgtg ccacatcccc gcgccctgga
      781 tcgccacctt cgcggtcagc acgacgtcgc cggtgagctt atattcaccc ggtggcaccc
      841 aaacaactcc tccccctcgc tccgccgccg cgatgcacgc gcgcaacgcc gccgtgtcgt
      901 ccgcctgacc gtcgccgacc gcaccgaaat cgaaaacaga aaacgcatcc gccggcgcag
      961 cgagcggcgg cggcacgttc tcgagatcga caaaatcgac gatgcaggga aacgcatccg
     1021 gtccgatttt ctcaacgcga acgacgtcgc ccgccgcaat gttcagatcc ttcaagcgaa
     1081 tctcatcgta gaaatttcgc ggcttctcat cgcctggccg attcgagaac ggatagtttc
     1141 cataaagcca cgaataatgc gacgtcagcg tcagtgttcg caccgcgcgg ccattgacga
     1201 aaacactcag cgccgattcc acgcctccac ccgcggtcgc atcccgcaga ctgtagcgga
     1261 cgaccatcgc atttgcgtcg gcccccgccg cgaattcaac gaactcgccg ctactcccga
     1321 gcctcacgca acgctgtccg gacgcttcgg tttcgacgcg gtgcggatcg tagcgtggcc
     1381 ccaggatctc gccggtgcct ttcatcgcct cggcttcgta ggtcgtccac gtcacgttgg
     1441 caccgaccgc atcgcccgcc cgcatcgcca acgccgcaca tccggcccca aggagaagca
     1501 acgctgtagt tgtgattctc atacgctcac ggtcacagat ttgcccggca ggcattttac
     1561 ttttcgtcca gcgatcacgg cacggtgcgc gcgtgcattg cttgccgccg gcgcgatcgt
     1621 caccgccgac ttcgtgatgc tcacgcgcag ccggtcgccg cgataatcga ggttaaactc
     1681 cagcttccgc catttccggc agaggcgcgg cgcaatcgtg attccttgct cgtcgacctg
     1741 cacgccaccg aaaccgaaca ccgccgtcat ccacgcgccg ccattcgcgg ccggatgcgt
     1801 gccgcccatg aaaacggtgc cggcatacac cttgtatttg gcttccaggt cgatcttcgc
     1861 cgtcttcaga aaatactcgt aggcgaaatc cacgtcgccg aactccgccg ccaccatcgc
     1921 atacgcgcac gcgctgaggc tcgaaccgtt ctcc
//
LOCUS       DASPLT010000230         1319 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_104679, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000230 DASPLT010000000
VERSION     DASPLT010000230.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1319)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1319)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1319
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_104679"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            52..>1319
                     /locus_tag="VEA63_02465"
     CDS             52..>1319
                     /locus_tag="VEA63_02465"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HYD82876.1"
                     /translation="MFVQAPENAEAELGSLPGVAYVERLRPLKRHLSRALDLSNVSAA
                     WSAVNGPQNAGAGVRIAIIDTGIDHTHPAFEDSGLQYPAGFPKCQERSGDCAYVNRKV
                     IVARSYVSMLVGSDPVETRPDDLSPRDRVGHGTAVAMIAAGRRNTGPAGTIEGVAPAA
                     FLGNYKVFGSPGVNDRYTYDDVIIQAMDDAIADGMNVAVLSLGSPAFWGPRDRGATCD
                     FEGTRACDWRAEAAEYAGRLGLTLVVSAGNTGDVSTRWPAFNSVETPGTAPSAITVGA
                     ITNSHILYQSLTVEGPNVPANVRRLNTLFGNGPRPGGPLTAEVRDAGSACEPLGNGTL
                     QGVFALIPRGDCQLALVVQNAQRAGAIGAIIYQREGFQGVFPMRGLEETGIPAVLIGN
                     QDSTALKTYIASNPNARVTLDPALTSVQTT"
BASE COUNT          247 a          420 c          422 g          230 t
ORIGIN      
        1 aaccagagag ggtttcgcgt taccgcagcg gagcaactgc tcaccaatgc cgtgtttgta
       61 caggcaccgg agaacgcgga agccgaactc ggcagtctgc ccggcgtcgc ctacgtcgag
      121 cggctccggc cgctgaagcg acatctcagc cgcgcgctcg atctgtcaaa cgtgagtgcg
      181 gcgtggtcgg cggtgaacgg gccgcaaaac gcgggagcag gcgtccggat tgcgatcatc
      241 gacaccggta tcgatcacac gcatcccgcg ttcgaggaca gcggtctgca atacccggcg
      301 ggatttccga aatgccagga aaggagcggc gattgtgcgt atgtgaaccg gaaggtgatc
      361 gtcgctcgaa gttacgtgag catgcttgtc ggaagcgacc ccgtcgaaac ccgcccggac
      421 gatctgtccc cgcgtgatcg cgtagggcac ggcaccgcag tcgccatgat cgccgcgggc
      481 cgccgcaaca ccgggccggc cggcaccatc gaaggcgtcg caccggctgc gtttctcggc
      541 aattacaaag tgttcggctc gccgggcgtg aacgaccgtt acacgtatga cgacgtcatc
      601 attcaggcga tggacgacgc gattgcggac ggcatgaacg tcgcggtcct ttccctcggc
      661 tcgcctgcat tctggggtcc ccgcgatcgc ggcgcaacgt gcgactttga agggactcga
      721 gcctgcgact ggcgcgcgga agccgctgag tacgccggcc gcttaggcct gacgctggtg
      781 gtctctgcgg gcaatacagg cgatgtctcc acccggtggc cggcgttcaa ctccgtggaa
      841 acgccgggga ccgcgccgtc ggcgatcaca gtgggtgcca tcaccaactc tcacattctc
      901 tatcagagtt tgaccgtcga aggaccaaac gtgccggcga acgtccgccg tttgaacacg
      961 ctgttcggca acggacctcg tcccggcggt ccgctgacag ccgaggtgcg cgacgcaggc
     1021 tctgcgtgcg aaccactcgg taacggcact cttcagggtg tattcgctct catcccgcgc
     1081 ggcgactgcc agcttgcgct cgtggtccag aatgcacaac gcgccggagc gatcggtgcc
     1141 attatctacc agcgtgaggg tttccagggc gtgtttccga tgcgaggcct ggaagaaacc
     1201 ggcatcccgg cggtgctgat tgggaatcaa gattccaccg cgctgaagac gtacatcgcc
     1261 tccaacccga atgcgcgggt cacgctcgat ccggcgctca catcggttca aacgactga
//
LOCUS       DASPLT010000231         1009 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_146521, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000231 DASPLT010000000
VERSION     DASPLT010000231.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1009)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1009)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1009
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_146521"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..777
                     /locus_tag="VEA63_02470"
     CDS             <1..777
                     /locus_tag="VEA63_02470"
                     /inference="COORDINATES: protein motif:HMM:NF012669.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HYD82877.1"
                     /translation="VPLHIVEQRREKLRHLIRTDGFIPIAEICRRLDVSEATARRDLA
                     SIAANGHITRTRGGALADYNTTFASLGERAHRARTAKTRIATAALPLLPRKGTIFLDA
                     GTTIQALARLLLARRPAGELTLVTNSLPIASVIGGTSGIELHVLGGMFLHRQAVLLGP
                     HSVRALAAWKFDVAFLGGEGLDASGITNSHANIAAFQQAVLRHAAKTYFCLDATKLGR
                     STPHRVAGWDEPITLITDAPASDLAKAGIALPLTRYLPAK"
     gene            822..>1009
                     /locus_tag="VEA63_02475"
     CDS             822..>1009
                     /locus_tag="VEA63_02475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD82878.1"
                     /translation="MQRPPMGSLPLDKSPSGKLSAFDYMIVPDHICVASAKIRAARFP
                     TFWHAAQCDAARACHSPP"
BASE COUNT          170 a          381 c          273 g          185 t
ORIGIN      
        1 gtgccgcttc acattgtcga acagcgacgc gagaaattgc gtcacttgat tcgcaccgac
       61 ggctttattc ccatcgcgga aatttgtcgt cggctcgatg tatccgaagc aaccgcgcgc
      121 cgtgatctcg cgtcgatcgc cgccaatggc cacatcacgc gcacccgcgg cggcgcgctc
      181 gccgactaca acacaacctt cgcttcgctc ggcgaacgcg cccatcgcgc ccgcaccgcg
      241 aagacccgca tcgccacggc cgcgctgcca ctccttccgc gtaaaggcac gatcttcctc
      301 gacgccggca cgaccatcca ggcgctcgcg cgattgctgc tcgcgcgtcg tccggccggt
      361 gagctcacgc tcgtcaccaa cagcctgccg atcgcgagcg ttatcggcgg cacatccggc
      421 atcgagctac acgtcctcgg cggcatgttt ctacatcgcc aggccgtgct gctcgggccg
      481 cattcagtgc gcgcgctcgc cgcgtggaag ttcgacgtcg cgttcctcgg cggcgaaggc
      541 ctggacgcga gcggcattac caactcgcac gccaacattg ccgcgtttca gcaagccgtc
      601 ctgcgtcacg ccgcgaaaac ctatttctgc ttggacgcca ccaaactcgg gcgctccacg
      661 cctcatcgtg tggcgggctg ggacgagccg atcacgctga tcaccgacgc tcccgcctcc
      721 gatctggcca aagcaggcat cgcgcttccc ctcacgcgct acctgccggc caaataaccc
      781 gtcagacccg gcaagcgtag gccgcccgct cgctcgcgtt catgcagcgc ccgccaatgg
      841 gcagcttacc gctggacaaa tcgccttccg ggaagctctc cgcttttgat tatatgatcg
      901 ttcccgatca tatctgcgta gcttccgcca aaattcgcgc agcgcgtttt ccgacgttct
      961 ggcacgctgc acagtgcgat gccgcccgcg cctgccattc cccaccgtg
//
LOCUS       DASPLT010000232         4341 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_43609, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000232 DASPLT010000000
VERSION     DASPLT010000232.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 4341)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4341)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4341
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_43609"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..435
                     /locus_tag="VEA63_02480"
     CDS             <1..435
                     /locus_tag="VEA63_02480"
                     /inference="COORDINATES: protein motif:HMM:NF012759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HYD82879.1"
                     /translation="PLTPHNKIDRAALRALGTGATSTKAAHIPPRTPVEETIAAFWRE
                     VLKVDRVGAQDNFFDLGGHSLLATQVMWRVFEVFRLELPLRTLFEASTVTAFAAALTA
                     AVDQSTSQPGRAEKIARLHLRVSRMSPEEVQRTLAAKRTATA"
     gene            complement(548..1405)
                     /locus_tag="VEA63_02485"
     CDS             complement(548..1405)
                     /locus_tag="VEA63_02485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD82880.1"
                     /translation="MSASSSIRRVTGKTYSAEYFRIGEFDRLDAAEIVRVMRGQVAGC
                     IFRGVMNPALCQRVCDNFWKHPQRRQRGDAVPAYYLGTYHYGKELPAYFDDAQATRAA
                     VHEVFDGGRNVFSELMVSVRDELAQDGVNLRVASHEGRAAGEFVIRSWSGGGQFALAA
                     HDDAAQLSAKKQAGFEIQQVAASPLAAVNMCLQNGAAGGQLVYWNIEPDSETRRALGL
                     EETGYPYQAEWLEEFDRIDLPVHAGDIYFFNGKLIHAVQAQSQPGEYRSTISFLMGFK
                     DPQTAIYWA"
     gene            1680..3302
                     /locus_tag="VEA63_02490"
     CDS             1680..3302
                     /locus_tag="VEA63_02490"
                     /inference="COORDINATES: protein motif:HMM:TIGR01194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_function: GO:1904680 - peptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic peptide export ABC transporter"
                     /protein_id="HYD82881.1"
                     /translation="MNIARLLKEDAGAAVRSILWLALASGIANGVLLAIINSAAATTT
                     SSSPNVPHLVMFAVAIALFIYTKRLSLMRSAVMAEGIITRFRLRIGEKYRHSELQTAE
                     SIDKSVIESRLSQDTLTLSQAAGEIINAVQSAVMVLVCILYLAMLSVPAFVATLLMIG
                     AGISIYLANQKKLMAELHRSTQKEIEFFHTVNHLVSGFKELKMNRAKSDDLFNNTYRE
                     ISVSAETLKLKTGAEFIKNYLFSQTFFYILIGVLVFVLPTMSEAYGDVLTKSTAVILF
                     IIGPLTTMVGTIPLYSKANVAVDNLYKFEHFLDSRLAEKQSESALRFEDFRQIDLEKI
                     HFAYSNDPTAPGFAVGPFDFRIKRGETIFLIGGNGSGKSTLMKLLVALYRPVSGGISV
                     DGLRLQRQHYANYRELFSVIFSDFHLFDRLYGVHQVDERRVLELLKEMQLDDKTNFEN
                     GRFTNLNLSTGQRKRLALIIAMLDDRPILALDEFAADQDPSFRSYFYDVILKELKARG
                     KTVIAITHDDRYFGYADRVVKMEYGKLDTQQT"
     gene            3299..4282
                     /locus_tag="VEA63_02495"
     CDS             3299..4282
                     /locus_tag="VEA63_02495"
                     /inference="COORDINATES: protein motif:HMM:NF013324.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005743 - mitochondrial inner membrane
                     [Evidence IEA];
                     GO_component: GO:0035632 - mitochondrial prohibitin
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prohibitin family protein"
                     /protein_id="HYD82882.1"
                     /translation="MSDTSVPPSSAFGQDPAAAAAPVERITWYERKVDLFRHNFVEFF
                     QKHRVGLFICSLIFVFFLALFWTRIFIPINAGEAGVLFRRFGGGTVIDRIYGEGFHVI
                     FPWNRMTVYNVRVQERTTLVEALSSNGLKIHVTVSMRFLPQYILLPHLHQKIGPNYVE
                     KIVLPEVIESVRGVIGGYRPEELYTAKISEVSHAVLANATRELTDKYLRFDNVVVRKI
                     ELPALVVTAIENKLTQEQLFQEYEFRIPREKKEAERKLIEAEGIRAFQEKITSGLTDT
                     YLRYRGVEATLDLAKSHNAKVIVIGGKDGMPLILNPDSTPPTSPPPIPLRR"
BASE COUNT          863 a         1479 c         1160 g          839 t
ORIGIN      
        1 ccgctcacgc cgcacaacaa aatcgatcgc gccgcgctcc gcgcgctcgg caccggcgcg
       61 acctcgacaa aagccgcgca catcccgccg cgcacgcccg tcgaagaaac gatcgccgct
      121 ttctggcgcg aggtgctgaa ggtcgaccgc gtcggcgcgc aggataactt cttcgacctc
      181 ggcggccatt cgctgctcgc cacgcaggtg atgtggcgcg tgttcgaagt cttccgcctg
      241 gagctgccct tgcgcaccct gttcgaagcc tcgaccgtca ccgccttcgc cgccgcgctc
      301 accgccgccg tcgaccagtc gacctcgcag cccggccgcg ccgaaaaaat cgcgcgcctt
      361 catctccgcg tcagccgcat gtcgcccgaa gaagtccagc gcaccctcgc cgccaaacgc
      421 accgccaccg cgtaaaaccg gtagggacgg ctctccgagc cgtccgcggc ccgtaggccc
      481 gggccctctg cgccgtgcct ttacgccccg actcgtcccg cgcaaccgct ccacccgccg
      541 cccaccttca cgcccaataa atcgccgtct gcggatcctt gaaccccatc aaaaacgaaa
      601 tcgtcgaacg atactcgccc ggctgcgact gcgcctgcac cgcgtggatg agcttcccat
      661 tgaaaaaata aatgtcgccc gcgtgcaccg gcagatcgat ccgatcaaac tcttccagcc
      721 actccgcttg atacggataa cctgtctcct cgagaccgag cgcacgacgc gtttcgctat
      781 ccggctcgat attccaataa accagctgcc cgcccgccgc gccgttttgc agacacatgt
      841 tcaccgcggc cagcgggctc gccgccactt gctgaatctc gaagcccgcc tgcttcttcg
      901 cgctgagctg cgctgcgtcg tcatgcgccg cgagcgcaaa ctgcccgccg ccgctccacg
      961 aacgaatcac gaactcgccc gccgcccgcc cctcgtgcga cgccacgcgc aggttcacgc
     1021 cgtcctgcgc gagttcgtcg cggaccgaca ccatcagctc gctgaaaaca tttcgcccgc
     1081 catcaaacac ctcgtgcacc gccgcgcgcg tcgcctgcgc gtcgtcgaaa tacgccggca
     1141 gctctttccc gtaatgatac gtgccgagat aatacgccgg caccgcgtcg ccgcgctgac
     1201 gccgttgcgg gtgcttccag aaattatcgc acacccgctg gcacagcgct ggattcatca
     1261 cgccgcggaa aatgcagccc gccacttggc cgcgcatcac gcgcacgatc tccgcagcgt
     1321 cgagccgatc gaactcaccg atgcggaagt attcggccga atacgttttt cccgtgacac
     1381 gccggatgct ggaagaagcg gacatgccgt tttttggcaa ctggccccgc gcatgtctag
     1441 cgttctcctc aggatttgcg cagcggagcg tcacgacgat gtaaacttcc cgccgcgctg
     1501 aaatcgttcc cacctccggt tgtcgctgga tgcgaagatg aggcgcgacg ctgataaaat
     1561 ttgccagccg caaaccaacc tccgtagcgt ccgcgaatgt tgatttggtt tggaaatggg
     1621 ccacggcacg gcggttggcg taaattcatt cccggccgtc tccacgtccg ctccatcgca
     1681 tgaacatcgc ccgattgctc aaggaagacg ccggcgcggc ggtgcgttcg attctctggc
     1741 tggcgctcgc ctcgggcatc gccaacggcg tcctgctcgc gatcatcaac tcggccgccg
     1801 cgaccaccac atccagttcg ccgaatgtgc cgcatctcgt gatgttcgcg gtcgcgatcg
     1861 cgttgttcat ttacacgaaa cgcctctcgc tcatgcgctc cgcggtgatg gcggaaggca
     1921 tcatcacgcg ttttcggctg cgcatcggcg agaaataccg ccactccgaa ctgcagaccg
     1981 cggagagcat cgacaaatcc gtgatcgagt cgcggctctc gcaggacacg ctcacgctgt
     2041 cgcaggccgc cggtgaaatc atcaacgccg tgcagtccgc ggtgatggtc ctcgtctgca
     2101 tcctttacct cgcgatgctt tccgttccgg cgttcgtcgc gacgttgttg atgatcggcg
     2161 cgggcatctc gatctatctc gccaaccaga aaaaactgat ggccgagctg catcgctcga
     2221 cgcagaagga gatcgagttt tttcacacgg tgaaccacct cgtcagcggc ttcaaggaac
     2281 tgaagatgaa ccgcgcgaag agcgacgatc tcttcaacaa cacctatcgc gagatcagcg
     2341 tttcggcgga aacgttgaag ctcaaaaccg gcgccgagtt catcaagaac tacctttttt
     2401 cgcagacgtt cttctacatc ctgatcggcg tgctggtctt cgtgctgccg acgatgagcg
     2461 aggcctacgg cgacgtgctc accaaaagca ccgcggtgat tcttttcatc atcggcccgc
     2521 tcacgacgat ggtcggcacc atcccgctgt attcaaaagc caatgtcgcg gtcgataatc
     2581 tctacaagtt cgagcacttc ctcgacagcc ggctggccga aaaacaatcg gaatccgccc
     2641 tccgcttcga ggacttccgc caaatcgatc tggagaaaat ccacttcgcc tactccaacg
     2701 acccgaccgc gcccggcttc gccgtcggac ccttcgactt tcgaatcaag cgcggcgaaa
     2761 ccatcttcct catcggcggc aacggctcgg gcaaatcgac gctgatgaag ctgctcgtcg
     2821 cgctttatcg cccggtcagc ggcggcatct cggtggacgg cctgcgattg cagcggcagc
     2881 actacgccaa ctatcgcgag ttgttctccg tcattttcag cgattttcac ctcttcgacc
     2941 ggctttacgg cgtgcatcag gtggacgagc gccgcgtgct cgagctgctg aaggaaatgc
     3001 agctcgacga taaaacgaat ttcgagaacg gacgcttcac caatctcaat ctttccaccg
     3061 gccagcggaa gcggctcgcg ctcatcatcg cgatgctcga cgaccgcccg attctcgcac
     3121 tcgatgaatt cgccgccgat caggacccgt cgttccgcag ttatttctac gacgtcattc
     3181 tgaaggaatt gaaggcgcgg ggaaaaaccg tcatcgccat cacgcatgac gacagatact
     3241 tcggatacgc cgatcgcgtc gttaaaatgg agtatggaaa actggatacc caacagacat
     3301 gagtgatact tccgtgcccc cttccagcgc cttcggccag gaccccgccg ctgcggccgc
     3361 gcccgtcgag cgcatcacgt ggtatgagcg caaggtcgat ctgtttcgcc ataacttcgt
     3421 cgagtttttt cagaagcatc gtgtcgggct gttcatctgc agcctgattt tcgtgttttt
     3481 tttggcgctg ttctggacgc gcattttcat cccgatcaat gccggtgaag ccggcgtgct
     3541 ctttcgccgc ttcggcggtg gcacggtgat cgaccggatt tacggcgaag gtttccacgt
     3601 gattttcccg tggaacagga tgaccgttta caacgtgcgc gtgcaggaac gcaccaccct
     3661 cgtcgaggcc ctgagttcga acggcctgaa gattcacgtc accgtgtcga tgcgcttcct
     3721 cccccaatat attttgctcc cgcatctgca ccagaagatc ggccccaact atgtcgagaa
     3781 aatcgtgctg ccggaagtga tcgagtcggt gcgcggcgtc atcggcggtt accgacccga
     3841 agagctttac accgcgaaaa tctccgaggt ttcgcacgcg gtgctggcca acgccacacg
     3901 cgaactgacc gacaagtatc ttcggttcga caatgtcgtc gtgcgcaaga tcgagctccc
     3961 ggcgctcgtc gtcaccgcga tcgaaaacaa gctcacgcag gaacagctct ttcaggaata
     4021 cgagttccgc atcccgcgtg agaaaaagga agccgagcgg aaactcatcg aggccgaagg
     4081 catccgggcg ttccaggaga agatcacgag cggtctcacc gacacctacc ttcgttatcg
     4141 cggcgtcgag gcgacgctcg atctggcgaa atcgcacaac gccaaagtca tcgtcatcgg
     4201 cggcaaggac ggcatgccgt tgatcctcaa tccggactca accccgccaa ccagcccgcc
     4261 cccgattccg ctccgacgct aggtcacacg cctacggtag cgtaggcgta gcgtcccacc
     4321 agaagcgtga ataaatcgac c
//
LOCUS       DASPLT010000233         1345 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_43619, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000233 DASPLT010000000
VERSION     DASPLT010000233.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1345)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1345)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1345
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_43619"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..461)
                     /locus_tag="VEA63_02500"
     CDS             complement(<1..461)
                     /locus_tag="VEA63_02500"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF014502.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009341 - beta-galactosidase complex
                     [Evidence IEA];
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HYD82883.1"
                     /translation="MKTDGKGKPILGRGWADRPVAAMLGSLSIWVGSCIPPAEPEEPN
                     AWSSVAIGLCEDYPEETRSLARARKDLIAAREAGAAVLRIAFGWDAIEPERGVHDWSF
                     WDEFVPMAAREFGLRLIPYVCYTPRWAATDAGPEHWRSPPRDAEDFGRFMAV"
     gene            783..>1345
                     /locus_tag="VEA63_02505"
     CDS             783..>1345
                     /locus_tag="VEA63_02505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD82884.1"
                     /translation="MPTLPLRWFLMLVGLFALVGATDLYFSHVSRQSQLTAADSRIDA
                     ADFFAAARAGELKEGHIVYRGHALGLAELVATRTVDGTTRTVRSAARLTDADLLLLRE
                     RRFLEEDTATYAAKKATAAELTAKIAHGAVAPIGIITLLAGIIVVAQRYAGRFTAFSS
                     RRLQPVTSVVKFSSVAGCDEAKDEVHEV"
BASE COUNT          235 a          509 c          347 g          254 t
ORIGIN      
        1 accgccatga aacgtccaaa atcttccgca tcccgcggag gcgaacgcca gtgctccggc
       61 cccgcatcgg tcgctgccca gcgtggcgtg taacacacat acggtatcaa acgcagcccg
      121 aactctcgcg ccgccatggg cacgaactcg tcccaaaaac tccaatcatg cacgccgcgc
      181 tccggctcga tcgcatccca accgaacgct attcgcagca ccgccgcacc cgcctctcgc
      241 gccgcgatca aatccttccg cgcgcgcgcc agcgaccgcg tctcctccgg ataatcttcg
      301 cagagtccga tcgcgacaga gctccatgca ttcggctcct cgggctcagc cggtgggatg
      361 cagcttccga cccaaatgga aagcgatccc agcatcgcag ccacaggccg gtccgcccac
      421 ccccgcccca aaatcggttt acctttgccg tcggttttca cgcgctcctc cttgtgacac
      481 ggtcaggtcc agccgagctc aactcgcccc acccacccgc ctccgacctc tcggacaggc
      541 cggagtgcga ttttctcctt ttttacgcgc aaccactttc aggcacttgc gcaatttccg
      601 gtcgttcgat cgcgcctgct tcccgcgcgc aactcacgcg cgcatcgcct gctacactca
      661 cccgtctcag atcacgtttc gcctgcgcaa ttgcataggc gttctcggag aaggtggctc
      721 ggtcctcggc tgcgacccaa tccgtggttg tggggattca cccgatatgt cgcataggat
      781 gaatgcccac actccctctg cgttggttcc tgatgctggt cggactcttc gccctcgtcg
      841 gcgcgacgga tctttatttc tcccatgtct cccgccagtc ccaactcacc gcagcggact
      901 cacgcatcga tgccgcagac tttttcgcgg cggcacgcgc gggtgaattg aaagaagggc
      961 acatcgttta tcgcggacac gcgctcgggc tggccgaact cgtcgccaca cgcacggtcg
     1021 acggcacgac gcgcacggtg cgctccgccg cccggctcac ggacgccgat ctgctcctcc
     1081 tgcgcgaacg ccgcttcctc gaggaagaca ccgccaccta cgccgcgaaa aaagccactg
     1141 ccgccgagct caccgccaag atcgctcatg gcgccgttgc cccgatcggc atcatcacct
     1201 tgctcgccgg catcattgtc gtggcccagc gttatgcggg tcgcttcacc gcattctcca
     1261 gccgccgcct ccagcccgtg acgtcggtcg tcaaattttc ctctgtcgcc ggctgcgacg
     1321 aagcgaagga cgaggttcac gaggt
//
LOCUS       DASPLT010000234         1671 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_208256, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000234 DASPLT010000000
VERSION     DASPLT010000234.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1671)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1671)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1671
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_208256"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..347)
                     /locus_tag="VEA63_02510"
     CDS             complement(<1..347)
                     /locus_tag="VEA63_02510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007360363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HYD82885.1"
                     /translation="MEAALDQPVLVLNRLWQAVNVIGAKRAFGLLARGHAHVVHHHDD
                     EFRTFQLMDWIDFSQDNPPLVALEMVHTPSRTIRLPRVILLTFFDKLPCKELKLTRNN
                     IFERDKNTCQYCAR"
     gene            complement(545..>1671)
                     /locus_tag="VEA63_02515"
     CDS             complement(545..>1671)
                     /locus_tag="VEA63_02515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HYD82886.1"
                     /translation="RSKIEALTRLIDVHDFRYGIIFCSTKIMVDDLDEHLHSRGYATD
                     RLHGDISQAQRDRVMDKFRRKGFEFLVATDVAARGLDVDDLEVVFNFDLPNDAEDYTH
                     RIGRTGRAGKSGRAFTFVSGQEIYKLQSMVRWAKLNIRRGKLPSLDEVEEARTNVFFE
                     KIRATLDAKEFRPHDRMIDRLLDQGYASTDICSALIHLLQGAPGGGGDKKAASGKKAA
                     GAAPAPVEQRMTVAPPAAPAKSPPTWVKQRAEPKSTARVPVDEIAAGAPVDDAEEETE
                     RPKKSKYERPARTGREPGMTTIFLNVGRKQLVTPADIVGKISGVTRLSPNVVGAIDIH
                     QRHTLVDVAEAEAPVIVKKLAGIKVKGIELAPALTSEGQK"
BASE COUNT          301 a          583 c          441 g          346 t
ORIGIN      
        1 cgcgcgcaat actggcaggt gttcttgtcg cgctcgaaga tgttgttgcg cgtgagcttc
       61 agctccttgc acggcagctt gtcgaaaaac gtcagcagga tcactcgcgg caggcgaatc
      121 gtccggctcg gtgtgtgcac catctcgagc gccaccagcg gcggattgtc ctgcgagaaa
      181 tcgatccagt ccatcaactg gaatgtccga aattcatcgt catgatgatg caccacatgc
      241 gcatgaccgc gcgccaacaa gccaaacgcc cgcttcgcgc cgatgacatt cactgcctgc
      301 cacaggcggt ttaaaaccag caccggttga tcgagcgcgg cttccatacg gggccatggc
      361 aataatggcc cgccgcgctc gctgtcaaga atgccaagcc cgcgcgagcg gcggaaagct
      421 ccaaattcca agctccaagc tacagagaaa cctcaaaaac ccattcaacg acccagccac
      481 tttggccatt cggtgcttgg aatttggatc ttcactggag cttggatctt ggagcttggt
      541 gctttcattt ctgaccttcg ctcgtcagcg ccggagccag ctcgatgcct ttcactttga
      601 tcccggcgag cttcttcacg atcaccggcg cctccgcctc cgccacgtcc accagcgtgt
      661 gccgctgatg aatatcgatc gcgccgacca cattcgggct aagccgcgtc acgccggaaa
      721 ttttccccac aatatccgcc ggcgtcacga gctgctttcg tccgacattc aggaaaatcg
      781 tcgtcatccc cggctcgcga cccgttcgcg ccggccgttc gtatttcgac ttcttcggcc
      841 gctctgtctc ctcctcggca tcatccaccg gcgcacccgc cgcaatttcg tccaccggca
      901 cgcgcgccgt gctcttcggc tccgcgcgct gtttgaccca cgtgggcggc gatttcgccg
      961 gagccgcagg cggcgcgacc gtcatgcgtt gctcgacggg ggccggggcg gcgcccgccg
     1021 ccttcttccc gctcgccgct ttcttgtccc cgccgccccc cggcgcgcct tgcaacagat
     1081 gaatcagcgc tgagcagata tccgtgctcg cgtagccctg atcgagcaat cggtcgatca
     1141 tccgatcgtg cggacgaaac tccttcgcat cgagcgtcgc gcgtatcttt tcgaagaata
     1201 cattcgtccg cgcctcctcg acctcgtcga gcgacggcag ctttccgcgc cggatgttca
     1261 acttcgccca gcgcaccatg ctttgcagtt tgtagatctc ctgccccgac acgaacgtga
     1321 acgcccgtcc cgactttccc gcgcgtcccg ttcggccgat ccggtgcgtg taatcctccg
     1381 cgtcgttcgg caggtcaaag ttgaacacca cttcgagatc gtccacgtcc agcccgcggg
     1441 ccgcgacgtc cgtggccacg agaaattcga aacccttccg tcgaaattta tccatcacgc
     1501 ggtcgcgctg cgcctgcgag atatcgccgt gcagccggtc cgtcgcatac ccgcgcgagt
     1561 gcaggtgctc atccagatcg tcgaccatga tcttcgtgct gcaaaaaata atcccgtaac
     1621 gaaaatcgtg cacgtcgatc aatcgcgtca gcgcctcgat cttcgagcgc c
//
LOCUS       DASPLT010000235         1731 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_25287, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000235 DASPLT010000000
VERSION     DASPLT010000235.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1731)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1731)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1731
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_25287"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(64..1095)
                     /locus_tag="VEA63_02520"
     CDS             complement(64..1095)
                     /locus_tag="VEA63_02520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012377077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HYD82887.1"
                     /translation="MKPTATLEDVAKAAGVHRTTVSLSLRNHPRIPEETRNRVKAVAE
                     RLRYRINPLVSALMQSRRSGKPVKHATLAYVTNYPTRYGWRPEHHDRPDFFPGAVERA
                     GDFGYKLEHFWLGEPGMTPNRFCDMLSARSINGLIIGRLPPGQNSLQLDWDRFSCVAL
                     GMTLRSPVLHHVTENHYDTVWQSMERCLERGYKRVGIVFPDGNDSPRVGDRWLGAFFT
                     QQLKFAPRDRIPLCSEKPTDEASFRAWFLKHKPDAILVNNARLLIQWLEQLGRKVPED
                     VGIVSLEHRRDFEGTGMYYDPAKIGGLAVEMLIGLMHRNETSVPAVQHEILLTGEWRE
                     NRCLPTRDS"
     gene            complement(1335..>1731)
                     /locus_tag="VEA63_02525"
     CDS             complement(1335..>1731)
                     /locus_tag="VEA63_02525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="lysophospholipase"
                     /protein_id="HYD82888.1"
                     /translation="AENMGRYVDEVRALGAQPILVTSLTRREFDPARPDKIGGTLQPY
                     VEAVRKVAAEKHVPLIDLYARSVEFCERLGPDGTAQFNLNTDQGIDRTHLDAAGSLVF
                     ARLVVDELRRAVPALAPHFPSSGDSVSFF"
BASE COUNT          315 a          464 c          617 g          335 t
ORIGIN      
        1 tctccttgcg gcgcaggcat tctcgtcgat agcccgcgat gatttatcgt ttctccagtc
       61 cgctcaggag tcgcgggtcg gcagacagcg attttccctc cattcgccgg tcagcaaaat
      121 ctcgtgctgc acagcgggga cgctggtttc gttccggtgc atgaggccga tgagcatctc
      181 gacggcgagg ccgccgattt tcgcgggatc gtaatacatg ccggtgccct cgaaatcacg
      241 gcggtgttcg aggctgacga tgcccacgtc ctcgggcact ttgcggccga gttgttcgag
      301 ccactggatc agcaggcgcg cgttgttcac gaggatcgcg tcgggtttgt gcttgaggaa
      361 ccaagcgcga aacgaggctt cgtcggtggg cttttccgag caaagcggga tgcggtcgcg
      421 tggcgcgaat ttcaactgct gcgtgaaaaa agctccgagc cagcgatcgc cgacgcgcgg
      481 gctgtcattg ccgtcgggaa acacgatgcc gacgcgtttg tagccgcgtt cgaggcaacg
      541 ctccatcgac tgccacacgg tgtcgtagtg attttccgtg acgtggtgaa gcaccggcga
      601 gcgtagcgtc atgccgaggg cgacgcagga aaaacgatcc cagtcgagct ggagggaatt
      661 ctggccgggc ggaagacggc cgatgatgag gccgttgatg ctgcgcgccg acagcatgtc
      721 gcagaagcga ttcggcgtca tgccgggctc gccgagccag aagtgttcga gcttgtagcc
      781 gaagtcgccc gcgcgctcga cggctccggg gaaaaaatcc ggccggtcgt ggtgctccgg
      841 acgccagccg tagcgggtgg ggtaattggt gacgtaggcg agcgtggcgt gcttgacggg
      901 cttgccggag cgccgcgatt gcatgagcgc ggagacgagc ggattgatcc ggtagcggag
      961 tcgctcggcg acggctttga cgcggttgcg ggtttcctcg gggatgcgcg ggtgattgcg
     1021 caaagaaaga gagaccgtgg tgcgatgaac gccggcggcc ttggctacgt cctcgagtgt
     1081 cgcggtgggc ttcacgagcg gagtcaacgg ggagcgcaga gcggcagtcg agcgaaatgt
     1141 gactgggaac gaccgctgcg ttgagagggt ttggagttct tcgaccggtg gttcgatggc
     1201 tcgctcatca ccgtagggaa cgatcacgca aagatcgcgg aaaaggcaga ggatgtttcc
     1261 gcggccacct gacgttcgcg caatttttct gcctccattt ttctgaccga tcagctgagg
     1321 tcagaaaaat agggtcagaa aaatgaaaca gaatcgcctg aacttgggaa atgcggagcg
     1381 agcgcgggga cggcgcggcg gagttcgtcg acgaccagac gggcgaagac aaggctgcca
     1441 gcggcgtcga ggtgcgtgcg atcgatgcct tggtctgtgt tcaggttgaa ctgcgcggtg
     1501 ccgtcggggc cgaggcgttc acagaattcg acgctgcgcg catagagatc gatgagtggc
     1561 acgtgttttt ccgcggcgac ttttcggacg gcctccacgt agggctggag cgtgccgccg
     1621 attttgtcgg gccgcgcggg atcgaattcg cggcgcgtga gggaggtcac gaggattggt
     1681 tgcgcgccca gcgcccgaac ctcgtcgacg taacgcccca tgttttcggc g
//
LOCUS       DASPLT010000236         1079 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_248805, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000236 DASPLT010000000
VERSION     DASPLT010000236.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1079)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1079)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1079
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_248805"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..502
                     /locus_tag="VEA63_02530"
     CDS             <1..502
                     /locus_tag="VEA63_02530"
                     /inference="COORDINATES: protein motif:HMM:NF013703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HYD82889.1"
                     /translation="AQILMAHWAGARRFEDVDRLLWRALRRAMLFAFLYALAAWFLAD
                     HVLGFFTTDPAIKQLGETLLLIAIFYEPARAVNIIVGFSLKTVGDARFPLIVGMVFIW
                     GILPVAFFIDRMWGMTVVGFWLCFAADEIVRAFINLWRWRTGKWKAMGIALPEKPPSE
                     VLPMEA"
     gene            608..>1079
                     /locus_tag="VEA63_02535"
     CDS             608..>1079
                     /locus_tag="VEA63_02535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HYD82890.1"
                     /translation="MTLTRQTILVVEDEVAIAETIIYGLQTEGFAVVWKTTGREALAI
                     LNAQPIALVVLDVGLPDASGFDVCRELRKTHAVPVIFLTARSAEVDRIVGLELGADDY
                     VAKPFSPRELTARVRAVLRRMTGSALANGNANTAASDGPWSHDEARCRISYRGRP"
BASE COUNT          174 a          293 c          382 g          230 t
ORIGIN      
        1 cgcgcagatc ctgatggcgc attgggcggg ggcacggcgg ttcgaggacg tcgatcggct
       61 gctctggcga gccctgcgac gggcgatgct cttcgcattt ctgtatgcgt tggccgcgtg
      121 gttccttgcc gatcatgtgc tcggtttttt cacgaccgat cccgcgatca agcagctcgg
      181 cgagacgctg ctgttgattg cgattttcta cgagcccgcg cgagcggtga acatcatcgt
      241 cggtttcagc ctgaagaccg tcggcgacgc gcggtttccg ctgattgtcg ggatggtgtt
      301 catctggggc attctgccgg tggcattctt tatcgaccga atgtggggca tgacggtggt
      361 cggtttctgg ctgtgtttcg ccgccgatga aatcgttcgc gcgttcatca acctctggcg
      421 ctggcgcacg ggcaaatgga aggcaatggg catagcgctt ccggaaaagc cgccgtcgga
      481 ggtgttgccg atggaggcgt gagggtgggg gcgcgtgtcc tcacgcgtcc tgggcctcac
      541 ctcatcgtgg ggacgattgt gccggaaact tgcgtcgctc gcctgcgcat cgcattcaaa
      601 acggcgcatg acgctgacgc ggcagacgat tctggtggtc gaggatgaag tggcgatcgc
      661 cgaaacgatt atttatgggt tgcagacgga aggtttcgcg gtcgtttgga aaacaacggg
      721 acgcgaggcg ctcgcgattt tgaatgcgca gccgatcgcg ctcgtggtgc tcgatgtcgg
      781 cctgccggat gcgagcggct tcgacgtctg tcgggagctg cgaaaaacgc atgcggtgcc
      841 cgtgatcttt cttacggcga gaagcgccga ggtggaccgc atcgtcggcc tggaactcgg
      901 cgccgacgat tacgtggcga agccgttcag cccgcgcgaa ctgacggcgc gcgtgcgcgc
      961 ggtgttgcga cgaatgaccg gcagtgcgct cgcaaatgga aacgcgaaca ctgcggcgtc
     1021 ggatgggccg tggtcgcacg atgaagcgcg ttgtcggatc agttatcgcg gtcggccgc
//
LOCUS       DASPLT010000237         1002 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_84949, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000237 DASPLT010000000
VERSION     DASPLT010000237.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1002)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1002)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1002
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_84949"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..705
                     /locus_tag="VEA63_02540"
     CDS             <1..705
                     /locus_tag="VEA63_02540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HYD82891.1"
                     /translation="VKPGQCVAILGATGAGKSVLMSLIPRFYDPTSGSVLLDGIDARR
                     LHLDDLRRNIGLVFQESFLFSNTIAANIAFGHPDATPAQIEKAARIAAAHDFITALPK
                     GYDTILGESGSTLSGGQRQRLAIARAVLLEPAILLLDDPTAAIDSETEHEIFEALDRA
                     IAGRTTFIVAHRLSTLRRADFIIVMENGRIVQRGTHQELMRVPGPYLRVARLQLVDAR
                     DLQVGRGVPAEPPTSP"
     gene            702..>1002
                     /locus_tag="VEA63_02545"
     CDS             702..>1002
                     /locus_tag="VEA63_02545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HYD82892.1"
                     /translation="MKPSDPKPTNVLVHRSRDDDDDEQFKPLEWGLIRRLFGYTHVVA
                     RKRNWLIVLSAIRSVQLAALVWLAAEIINGPIAHRDSPGLILGIAGYALLAIATDG"
BASE COUNT          187 a          410 c          245 g          160 t
ORIGIN      
        1 gtgaaacccg gccaatgcgt cgcgatcctg ggcgcaaccg gcgccggaaa aagcgtcctg
       61 atgagcctga tcccgcgctt ctacgatccg acctccggca gcgtgctcct cgacggcatc
      121 gacgcgcgcc gtctccacct cgacgacctc cgccgcaaca tcggcctcgt tttccaagag
      181 agctttctct tctccaacac catcgccgcc aacatcgctt tcggccatcc cgacgcgacg
      241 ccagcgcaaa tcgaaaaagc cgcacgcatc gccgccgcgc acgacttcat caccgcactg
      301 ccgaaaggct acgacaccat cctcggcgaa agcggcagca cgctttccgg cggacaacgc
      361 cagcggctcg ccatcgcgcg cgccgtgctt ctcgagcccg cgatcctgct cctcgacgat
      421 cccaccgcag cgatcgactc cgaaaccgaa cacgaaattt tcgaagccct cgaccgcgcc
      481 atcgccggac gcaccacgtt tatcgtcgcg catcgcctca gcaccctgcg ccgcgccgac
      541 ttcatcatcg tcatggaaaa cggccgcatc gtgcagcgcg gcacgcacca ggaattgatg
      601 cgcgtccccg gcccttacct ccgcgtcgcc cgccttcaac tcgtcgacgc ccgcgacctc
      661 caggtagggc gaggcgtccc cgccgagccg ccaacctcgc cgtgaaacct tccgacccaa
      721 aacccaccaa cgtcctcgtc catcgctccc gcgacgacga tgacgacgag caattcaaac
      781 cgctcgaatg gggactcatc cgtcgtctct tcggctacac gcacgtcgtc gcacgcaaac
      841 gcaattggct catcgttctc tccgccatcc gctcggtgca actcgccgcg ctcgtgtggc
      901 tcgccgccga aatcatcaac ggccccatcg ctcatcgcga ttcccccggc ctcatcctcg
      961 gcatcgccgg ctacgcgcta ctcgcgatcg ccaccgatgg cc
//
LOCUS       DASPLT010000238         1101 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_64154, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000238 DASPLT010000000
VERSION     DASPLT010000238.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1101)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1101)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1101
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_64154"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1019
                     /locus_tag="VEA63_02550"
     CDS             <1..1019
                     /locus_tag="VEA63_02550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ferrous iron transporter B"
                     /protein_id="HYD82893.1"
                     /translation="IFLPQILILFFFIGLLESTGYMARAAFIMDRLMSRVGLNGKSFI
                     PLLSSYACAIPGIMATRTIEDSKDRLVTILVAPLMSCSARLPVYLLMIAALVPGDRVP
                     LLTKVGIMLLMYSLGTFGAFGFAWLFKRTLLKGEPPLMIMELPPYRMPKLKDVLMHMV
                     ERGWIFVRRAGTVILGISIVLWFLAAYPKASDTATPTEQLAQSFAGRAGHALEPVIKP
                     LGFDWQIGIGLISSFAAREVFVSTMSVVFNATSDDEEDTTPLRQAMLDARWPDGRPLF
                     TPLVCFTLMIFYVFAMQCMSTIAIVKRETNSWRWPLFQTAYMTGTAWILSLIVYQGGR
                     LLGF"
BASE COUNT          180 a          361 c          308 g          252 t
ORIGIN      
        1 tcatctttct accgcaaatc ctgattctct tcttcttcat cgggctgctc gaaagcaccg
       61 gctacatggc acgcgcggcg ttcatcatgg accgcttgat gagtcgcgtc ggcctcaacg
      121 ggaaatcctt catcccgctg ctgagttcct acgcgtgcgc cattcccggc atcatggcga
      181 cgcgcacgat cgaagattcg aaggaccggc tcgtcacgat tctcgtcgcg ccgttgatga
      241 gctgctccgc gcgcctgccg gtttacttgt tgatgatcgc cgcgctggtg ccaggcgatc
      301 gcgtgccgct gctcaccaag gtcgggatta tgcttctcat gtattccctg gggacgttcg
      361 gcgccttcgg cttcgcgtgg ctgttcaagc gcacgctgct caagggcgaa ccgccgctca
      421 tgatcatgga gctgccgccc taccgcatgc cgaagctgaa ggatgtgttg atgcacatgg
      481 tcgagcgcgg atggattttc gtccgccgcg ccggcaccgt catcctcggc atcagcatcg
      541 tgctctggtt tctcgcagcg tatccaaaag ctagtgacac cgcgacaccg accgagcaac
      601 tcgcgcaaag tttcgccggt cgcgccggtc acgcgctcga gccggtgatc aagccgctcg
      661 gcttcgactg gcagatcggc atcggcctca tcagctcgtt cgcggcgcgc gaagttttcg
      721 tttccacgat gagcgtcgtg ttcaacgcca cctcggacga cgaggaagac accacgccac
      781 tccgccaggc gatgctcgac gcgcgctggc cggacggacg cccgctcttc acgccgctcg
      841 tgtgtttcac gctcatgatc ttctacgtgt tcgccatgca gtgcatgagc acgatcgcga
      901 tcgtgaagcg cgaaacgaac agctggcggt ggccgctttt ccaaaccgct tacatgactg
      961 gcaccgcgtg gattttgtcg ctcattgtct atcaaggcgg ccgtcttctc ggcttctgag
     1021 ctgaatcttc aaaatcttcc gcgatgtctt ccgaactcca gtccgttctc gcgctgctcg
     1081 tcgtcgctct gaccgtcgtc t
//
LOCUS       DASPLT010000239         1324 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_167001, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000239 DASPLT010000000
VERSION     DASPLT010000239.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1324)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1324)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1324
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_167001"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1202)
                     /locus_tag="VEA63_02555"
     CDS             complement(<1..1202)
                     /locus_tag="VEA63_02555"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HYD82894.1"
                     /translation="MTTSSLVRLDLFSGRPLRPLRRILVQTLAIFAVGAGPVAEAAEL
                     AFPSTFKERMVITADGTEIFVRSGGAGPAAVLIHGYGDTGDMWVPLAVELAKTHTVIV
                     PDLRGMGKSSHPAGGYEKKAQAADIRAVVVALGGDRAAVVGHDIGNMVAYAYAASYPG
                     KVDRLVLMDAPIPGVGPWEEILKTPLLWHFNFGGPDAERLVQGRERIYLDRFWNEFAA
                     HPERIDEETRAHYAALYALPGGMRSGFAQFRAFTQDAEDNKVFVRTKLTMPVLAAGGE
                     KSFASVMAVVMRFAAVDVREAIIPDSGHWVIDENPGYTVALVSGFLRERESAASDRRL
                     TPNELKFVGNGAPGTGTSSVAGIQTVVLKGNPEQPGLYTIILRVPAHTKIAAHDHPDD
                     RVASVLSGT"
BASE COUNT          264 a          436 c          392 g          232 t
ORIGIN      
        1 gtccccgaaa gcacagacgc tacgcggtcg tccggatgat catgcgccgc gatcttggtg
       61 tgcgcgggaa cgcgcaggat gatcgtgtag aggccgggct gctcggggtt gcccttgagc
      121 accacggtct ggatgccggc gacggacgat gtgcccgtgc cgggagcgcc gttgccgaca
      181 aacttcagct cgttcggtgt gaggcggcgg tcggacgcag cggattcccg ctcgcgcaaa
      241 aatccgctga cgagtgcgac cgtgtatcca ggattctcat cgatcaccca gtggccggaa
      301 tccggtatga tcgcctcgcg cacatccacc gcggcgaaac gcatcacgac cgccatcact
      361 gaagcgaacg acttctctcc gcccgccgcg aggacaggca tcgtcaattt cgtgcgaacg
      421 aaaaccttgt tgtcctcggc gtcctgcgtg aaggcgcgga actgcgcaaa gcccgaacgc
      481 attccgccgg gcagcgcgta aagcgccgcg taatgagcgc gggtctcttc atcgattcgt
      541 tccggatgag cggcgaactc gttccagaaa cgatcgaggt agatgcgttc gcgaccttgc
      601 accaggcgct ccgcatccgg tccgccgaaa ttaaaatgcc acagcagcgg cgtcttcagg
      661 atttcctccc acggtccaac tccaggaatc ggcgcatcca tcagcacgag gcggtccact
      721 tttcccggat agctcgcggc ataagcatag gcgaccatgt tgccgatatc gtgccccacc
      781 actgcggcgc gatcgccacc caaggccacc accaccgcgc gaatatccgc ggcctgcgct
      841 ttcttttcat agccgccagc cggatgagat gattttccca tcccacgcag atcgggcacg
      901 atcacggtgt gcgtcttcgc cagctcgacc gcgagcggca cccacatgtc gcccgtgtcg
      961 ccgtaaccgt gaatcaaaac agccgccgga cccgctccgc cggaacgcac gaaaatctcc
     1021 gtgccgtccg cggtgatgac catccgttcc ttgaacgttg aaggaaaggc cagctcggcc
     1081 gcctccgcga ccggcccggc gccgaccgcg aaaatcgcaa gggtctgcac gagtattctg
     1141 cgcaacggcc gaagcggccg gccggaaaac aaatcgaggc gaacgagaga agaagttgtc
     1201 atgatttttc gagtcggcgc tgaaggttga atgtgcaggg tgcgatgcct cgctcgatca
     1261 gcgcgtgctc gcgcggcatg cttccatcta cgccgcaccg cttcgttctg aaaacgaagt
     1321 gccc
//
LOCUS       DASPLT010000240         1221 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_64161, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000240 DASPLT010000000
VERSION     DASPLT010000240.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1221)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1221)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1221
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_64161"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..927)
                     /locus_tag="VEA63_02560"
     CDS             complement(<1..927)
                     /locus_tag="VEA63_02560"
                     /inference="COORDINATES: protein motif:HMM:NF021492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HYD82895.1"
                     /translation="MKRSKTAWLVLGVLGVLVGVVGTWTWREHALATSVKTTLPTRPD
                     FAGKPMILGELLAQAGNKAESSKTRLEGMAELGRLYHANGFIAEAEACWQWLRSEQPR
                     EARWSYYLADSRRAKSDQPGMTAFLRETLDLAPDYAPARLQLANLQFKSGEPAEAQHN
                     YRLRLEALPQDPYARLGLARLALQAGQKDEAVAVLELLLKDTPHFSTAHNLYAELLAA
                     AGDDPGARKHRWLGRETLRYREAEDPWLDELLAWCYDYERLCVLGTIELQTEQHDRAH
                     AYFERAIGIDAGRSDAHELLASVYLKRGDARGA"
     gene            complement(924..>1221)
                     /locus_tag="VEA63_02565"
     CDS             complement(924..>1221)
                     /locus_tag="VEA63_02565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HYD82896.1"
                     /translation="AGAYSPNAADLNGDGTVDLLAVSCLADWSDPNSVSMMAWLNDGW
                     ENFTPVVLARRPIQLVSAAVGDLDGDNIPEIVTGAFHALPPFEETSNITLWRRK"
BASE COUNT          212 a          384 c          388 g          237 t
ORIGIN      
        1 ggccccacgc gcgtctccgc gtttcagata cacgctggcc agcaactcgt gagcgtcgga
       61 ccggcccgcg tcgatcccga ttgcccgctc aaaataagcg tgcgcccggt cgtgctgctc
      121 cgtctgaagc tcgatggtgc cgagcacgca gaggcgttcg taatcgtaac accacgcgag
      181 caactcatcc aaccaagggt cttccgcttc gcggtaacgc aaagtttcgc gcccgagcca
      241 gcgatgcttt cgcgcaccgg ggtcgtcgcc cgccgccgcg aggagttcgg cataaagatt
      301 atgggcggtg gagaaatgcg gcgtgtcttt taataggagt tcgagcacag caaccgcctc
      361 gtctttttgt ccggcctgaa gcgcgagccg cgccaagccg agtctcgcgt aagggtcctg
      421 cggcagcgcc tcgaggcgca accggtagtt gtgttgggct tccgccggtt cgccggattt
      481 gaactggaga ttcgcgagtt gcagccgggc gggagcgtag tcgggggcga ggtcgagtgt
      541 ttcgcgcagg aacgcggtca tgccaggctg gtcgcttttg gcgcggcgcg aatccgcgag
      601 atagtagctc caccgagctt cgcgcggttg ttcggaccgc aaccactgcc agcacgcttc
      661 agcttccgcg atgaatccat ttgcatgata cagacgcccg agctcggcca tgccttcgag
      721 ccgggtcttc gacgattccg ccttgttccc ggcctgagcc agcaactcac ccaaaatcat
      781 cggcttcccg gcgaagtcgg gacgcgtggg cagcgtcgtt ttcacgctcg ttgcgagcgc
      841 atgttcgcgc cacgtccacg tgccgacgac gcccacgagg acgccgagga cgccaagcac
      901 gagccaggct gttttcgaac gtttcacttt cggcgccaca aggtgatgtt actcgtctcc
      961 tcgaagggcg gcaacgcgtg gaaggcgccg gtcacgattt cgggaatgtt gtcgccgtcg
     1021 agatcgccca ctgccgcgct gacgagttgg attggtcgtc gcgcaagcac cacgggcgtg
     1081 aagttttccc agccgtcgtt taaccacgcc atcattgaga ccgagttcgg atcgctccaa
     1141 tcggcgagac aactgaccgc gaggagatcg acggtgccgt cgccgttgag gtctgccgcg
     1201 tttgggctgt aagcgccggc c
//
LOCUS       DASPLT010000241         1678 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_85022, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000241 DASPLT010000000
VERSION     DASPLT010000241.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1678)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1678)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1678
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_85022"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..497)
                     /locus_tag="VEA63_02570"
     CDS             complement(<1..497)
                     /locus_tag="VEA63_02570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation signal
                     domain-containing protein"
                     /protein_id="HYD82897.1"
                     /translation="MSSITRRQFVQKTALAAAAAQLASSLRAQAQVSGPASVSAAPRQ
                     PAKPGVAELRWLDGVAPKHQPGATWGVPWARGSQPAGATFALRTSAGESVPVQSWPLA
                     TWPDGSLKWTAHAVPANAGRAGETLELEAGAAVAPAVALTAQESDEAIEIDTGVMRVR
                     VAKRGR"
     gene            complement(686..1000)
                     /gene="rhaM"
                     /locus_tag="VEA63_02575"
     CDS             complement(686..1000)
                     /gene="rhaM"
                     /locus_tag="VEA63_02575"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0062192 - L-rhamnose mutarotase activity
                     [Evidence IEA];
                     GO_process: GO:0019301 - rhamnose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HYD82898.1"
                     /translation="MIRKAFVMSVHPGREDEYERRHRPIWAELERVLKAHGVHNYSIF
                     LHAETRQLFGYVEIEDEARWSAIAQTPECQRWWKHMADLMPSNPDNSPVSRELHEVFH
                     LD"
     gene            1343..>1678
                     /locus_tag="VEA63_02580"
     CDS             1343..>1678
                     /locus_tag="VEA63_02580"
                     /inference="COORDINATES: protein motif:HMM:NF012576.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HYD82899.1"
                     /translation="MAAPTLRTLARTLGLSRTTVSDALRGSPRVNPDTVARVREAAKA
                     AGYERNPLTGAVMSLLRRSRGQQFRGVLAAIEMVEPGRVAHAVRYNESLLAGVSARAD
                     ELGFKVERFE"
BASE COUNT          284 a          618 c          466 g          310 t
ORIGIN      
        1 cgaccgcgtt tcgccacccg cacccgcatc acgcccgtgt cgatttcgat cgcttcgtcg
       61 ctctcctgcg ccgtcaacgc caccgccggt gccaccgcgg cgcccgcctc cagctccaac
      121 gtttcccccg cacgccccgc attcgccggc accgcgtgcg ccgtccactt caacgatcca
      181 tccggccacg tcgccagcgg ccagctctgc accggcacgc tttcgcccgc actcgtgcgc
      241 agcgcaaacg tcgcacccgc cggctggctc ccccgcgccc acggcacgcc ccacgtcgct
      301 cccggttgat gcttcggcgc cacgccatcg agccagcgca attccgccac gccgggcttg
      361 gccggctgtc gcggcgcggc ggatacactc gccggtcctg aaacctgcgc ctgcgcacgc
      421 aacgacgaag ccagttgcgc cgccgcagca gccaacgcgg ttttctgaac aaactgacgg
      481 cgggtaatgg agctcatgga aaaaaggcga tgggtttcgg gttttgagtt ttgggttttg
      541 ggttctcgct ccgacctcgg agtttcccgc tcggagttcg gagttcagcg ttcagagtcc
      601 agcgttcgaa gttcaaattc ctcaatcccg ccccgccttg gaccacgtgg ctccgatcgt
      661 attcgaaccc gcaatacgcc cgtgttcagt ccaaatgaaa cacctcgtgc aactcccgtg
      721 acacggggct gttgtccgga ttgctcggca tcaggtcggc catgtgtttc caccagcgct
      781 gacactccgg cgtttgggcg atcgccgacc aacgcgcttc gtcctcgatc tccacataac
      841 cgaacaactg ccgcgtctcc gcgtgcagga agatcgaata gttatgaacg ccgtgcgcct
      901 tcaacacgcg ttcgagctcc gcccagatcg gacgatgccg ccgctcgtat tcgtcttcgc
      961 ggccgggatg cacggacatg acaaaagctt tgcgaatcat agggggaaac agggaaagca
     1021 ggcgcagcgt gcaacgcaat gactcgggtg cgaacggcgc ccgcggagga cagtccgaaa
     1081 aagaagcacc aagctccaac atccagggag ccatcagcct ccaaactcca ccctcgtcgt
     1141 cacgctggcg tttggaacgc ttggatcttc tttggacgtt ggagcttggt gcttggagct
     1201 tggatcttgg tgcttgtccg cagccgtttc actcttctcc caagcgggct gaagcccgcc
     1261 ctacctcgaa ccacaccgcg aaaatctgac gctccgattt gtcgttgaac ttcccgccgc
     1321 cgaccgaatc ctcggcggtc cgatggctgc ccccaccctg cgcactttgg cccgcaccct
     1381 cggcctctcc cgcaccactg tctccgatgc cctccgcggt tcgccgcggg tcaaccccga
     1441 caccgtcgcc cgcgtgcgcg aagccgccaa agccgccggc tacgagcgca atccgctcac
     1501 cggcgccgtc atgtcgcttc tccgccgttc gcgcggccaa caatttcgcg gcgtgctcgc
     1561 cgcgatcgaa atggtcgagc cgggccgcgt cgcgcacgcc gtgcgttaca acgaaagctt
     1621 gcttgccggc gtctccgcgc gcgccgacga actcggcttc aaggtcgagc gtttcgaa
//
LOCUS       DASPLT010000242         1236 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_187081, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000242 DASPLT010000000
VERSION     DASPLT010000242.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1236)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1236)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1236
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_187081"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1227
                     /locus_tag="VEA63_02585"
     CDS             <1..1227
                     /locus_tag="VEA63_02585"
                     /inference="COORDINATES: protein motif:HMM:NF033592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HYD82900.1"
                     /translation="EALEATGTVTVRKRRLPAERLVWLVVGMALFRNLPIEDVLRRLE
                     VALPAKSGKAPARSGVAQARKRLGADPIRHLFERTGEHWSSQSAERHLWRGLSLWAID
                     GTSLRVPDSPENRAHFGGRANQRSDSGYPLARVVTMMALRSHLMKAARIAPYNTSELA
                     LAHELFPEIPEGALAIVDRGFLYAELLVPLAADGKRQWLTRARKNTSYKVIKKLGPGD
                     ELVELNVTPEAKKNRPDLPSTWIARAIRYKRRGYKPQLLLTSLLDPKEYPRGEIVSLY
                     HDRWEIELGYDEVKTHMLDRMETLRSQSVAGVEQEIWGLLLAYNLVRLELEGAAAVAE
                     VAPVRMSFVAGLRFVRQTLEMAAVISPGRLPKYLENERLDLATFVLPPRRTDRLYPRA
                     VKIKMSHYARKRTAAK"
BASE COUNT          225 a          416 c          414 g          181 t
ORIGIN      
        1 gaagcgctcg aggccacagg caccgtcact gtccgcaagc gccgcctgcc ggccgagcga
       61 ctcgtgtggc tcgtggtcgg gatggcgctc ttccgcaacc tgccgatcga ggacgtgctc
      121 cggcggctgg aggtggccct cccggccaag agcggaaagg ctccggcccg gagcggagtt
      181 gcgcaggcga ggaagcggct tggagccgat cccattcgcc atctcttcga acgtacgggc
      241 gaacactggt ccagccaaag cgcggagcgg cacctctggc gcggactcag cttgtgggcc
      301 atcgacggca cctcgctgcg cgtgccggac tcgccggaga accgcgcgca cttcgggggc
      361 cgcgcgaacc agcgtagcga cagcgggtac cctctcgcgc gcgtcgtcac gatgatggcg
      421 ctgcgctccc acctcatgaa ggccgcgcgc atcgcgccgt acaacaccag cgagctggcg
      481 ctcgcccacg agctgtttcc cgagatcccg gagggcgcgc tcgcgatcgt ggaccgaggc
      541 ttcctctacg cggagctgct cgtgccactc gccgcggacg gcaagcggca atggctcacg
      601 cgagcccgga agaacacctc ctacaaggtc atcaagaagc ttgggccagg cgacgagctc
      661 gtcgagttga acgtgacgcc cgaagcgaag aagaaccgcc cggatctgcc gagcacctgg
      721 atcgcgaggg cgatccggta caagcggcgc ggctacaaac cccagctcct gctgacgtct
      781 ctgctcgatc ccaaggagta tccccgaggc gagatcgtgt cgctctacca cgatcgatgg
      841 gagatcgagc tcggctacga cgaggtgaag acgcacatgc tcgaccgcat ggagactctg
      901 cggagccaga gcgttgcggg agtcgagcag gagatctggg gcctgctcct cgcgtacaac
      961 ctcgtccggc tcgaactcga gggcgctgcc gccgtcgcgg aagtcgcccc ggtgcggatg
     1021 agcttcgtgg ctgggctgcg tttcgtgcgg cagacgttgg agatggccgc ggtgatcagc
     1081 ccgggcagac tccccaaata cctcgagaac gaacgcctcg atctcgcaac cttcgtgctg
     1141 ccgccccgcc gcacggatcg cctgtacccc agggccgtga agatcaagat gagccactac
     1201 gcccgaaagc ggacggcggc taagtgaacg gcattg
//
LOCUS       DASPLT010000243         1981 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_85037, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000243 DASPLT010000000
VERSION     DASPLT010000243.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1981)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1981)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1981
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_85037"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(249..911)
                     /locus_tag="VEA63_02590"
     CDS             complement(249..911)
                     /locus_tag="VEA63_02590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HYD82901.1"
                     /translation="MKGRLIAIGDIHGCHREFEDLLDKLALQRHDRVVLLGDLVNRGP
                     DSAAVIALARKHAHLSLLGNHELRLLNFRKTGDPTHLKKNDYDTLEQLGPKDWAYMNA
                     MPLTFHDRAHQIVLVHGGFLPSQPWQRQPARVVTRIQNIDKDGNPRKRSDAPNSPHWS
                     TLWRGPPFVVYGHTSRPEISRLKWSLGIDTACAMGGALTACILPEKKIVQVKAREKYY
                     EG"
     gene            965..1645
                     /locus_tag="VEA63_02595"
     CDS             965..1645
                     /locus_tag="VEA63_02595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD82902.1"
                     /translation="MELTLHPLAKHCRASGRAFAEGDRVVSHLVREETGEIARHDVLA
                     VEDANYAKPAFVFCSWTHPFKARRSEENPERTMKLTAENLFVTLADPTAEPNEENTPM
                     LQFLALMLERKKILKPRGMTPDRARQVYEHAKTHQRYEIPAGVLDEAFFVKIQGQLDL
                     LVGGPKKKAAGATPETAGAAEETASATAGESAANLQSGGEAVTKAESEAPAETSTDER
                     PKVDGASA"
     gene            complement(1757..>1981)
                     /locus_tag="VEA63_02600"
     CDS             complement(1757..>1981)
                     /locus_tag="VEA63_02600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA degradosome polyphosphate kinase"
                     /protein_id="HYD82903.1"
                     /translation="IEALFPIYDPEFKKRLIDELLASELRDNEDARELQSDGSYKLPA
                     RTASEPPFSAQRYFIAASLLRGSPPAAAAG"
BASE COUNT          367 a          483 c          778 g          353 t
ORIGIN      
        1 cgagtttttg cgggactcgc tcggtgaaca gcacgtcgat gccgaggccg acggcggact
       61 ggatcgcgaa ggcgcagcgt cgctgaagcg cttcgggtga ttgcattgcc ttcaggaaga
      121 cgggctgcag gtcgaggcag aggagaagga ggttggacga cgcgggcatg aaaaaggttg
      181 ggggtcgaac gcggagggag tgcgcgaggc gagtcggggc gtaaagcccc tcccacagtt
      241 cgggcagttc agccttcgta gtatttttcg cgggctttga cttggacgat tttcttttcg
      301 ggaagaatgc aggccgtgag tgcgccgccc atggcgcacg cggtgtcgat gccgagcgac
      361 catttgaggc gcgagatttc cgggcgcgag gtgtggccgt aaacgacgaa gggcggtccg
      421 cgccagaggg tggaccagtg cggcgagttg ggggcgtcgg agcgcttgcg gggattaccg
      481 tccttatcga tgttttgaat acgcgtgaca acgcgggccg gctgacgctg ccagggctgg
      541 ctggggagaa agccgccgtg aacgagcacg atttggtgcg cgcgatcgtg gaaggtgagc
      601 ggcatcgcat tcatgtaggc ccagtctttg gggcctagtt gctcgagcgt gtcgtagtcg
      661 ttcttcttca gatgagtggg atcgccggtt ttgcggaaat tgaggagtcg aagctcgtgg
      721 ttgccgagca gggagagatg cgcgtgcttg cgagcgaggg cgatgacggc ggcgctgtcg
      781 gggccgcggt tgacgagatc gccgaggagg acgacgcggt cgtggcgttg gagcgcgagt
      841 ttatcgagga gatcctcgaa ctcgcggtga cacccgtgga tgtcgccgat ggcgatgagt
      901 cggcctttca tgcgggtcgc ggaatttgac tagcgttcat tttttcgggg gctaaacaag
      961 gccaatggaa ctgacgttgc acccgctcgc caaacattgt cgcgcctccg gccgcgcgtt
     1021 cgccgagggc gatcgcgtcg tgtcgcatct ggtgcgcgag gagacgggcg aaatcgcgcg
     1081 gcacgacgtg ctggcggtcg aagacgcgaa ctatgcgaag ccggctttcg tattttgctc
     1141 gtggacgcat cccttcaagg cgcgtcgttc ggaggagaat ccggagcgaa cgatgaagct
     1201 gacggcggag aatctgtttg tgacgctggc ggatccgacg gcggagccga acgaggagaa
     1261 cacgccgatg ctgcaatttc tcgcgttgat gctggagcgg aaaaagattt tgaagccgcg
     1321 gggcatgaca ccggatcgag cgcggcaggt ttacgagcat gcgaagacgc atcagcggta
     1381 tgagattccg gcgggcgtgt tggacgaggc gttttttgtg aagatccagg ggcagctcga
     1441 tctgctggtc ggcgggccga agaagaaggc ggcgggggcg acgccggaga cggcgggtgc
     1501 ggcggaggaa acggcgagtg cgacggccgg ggagagcgcg gcgaacttgc agagtggcgg
     1561 tgaggcggtg acgaaggcag agagtgaagc gcccgcggaa acctcgacgg atgagcgccc
     1621 aaaggtggac ggagcgtcag cataaagggg cgggttgaac taaggacggc tcggagagcc
     1681 gcccccaccg gcgctgccgc gccattggtc ggcggcggcg agaggggaag cgcgcgcagg
     1741 ttgcccgtga tacgcgttaa ccggcggcgg cggcaggggg gctgccgcgc agcagggagg
     1801 cagcgatgaa gtagcgttgc gcggaaaacg gcggttcgct ggcggtgcgc gcggggagct
     1861 tgtaagagcc gtccgactgg agttcgcgag cgtcttcgtt gtcgcgaagt tcggaggcga
     1921 ggagttcgtc gatcagccgt ttcttgaact cgggatcgta tatgggaaag agcgcttcga
     1981 t
//
LOCUS       DASPLT010000244         1026 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_25364, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000244 DASPLT010000000
VERSION     DASPLT010000244.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1026)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1026)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1026
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_25364"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1019)
                     /gene="mutL"
                     /locus_tag="VEA63_02605"
     CDS             complement(<1..1019)
                     /gene="mutL"
                     /locus_tag="VEA63_02605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HYD82904.1"
                     /translation="MHVRDCGRPVGTRIEVTHLFNSVPARRKFLKTDQTEAAHIVQCV
                     RLYALACPRTAFTLIEDGRVIFRSPECATLAERIAEIFGRQTAEALVPLEAVETGMRL
                     SGLIGRPGIGRGTRHEMIVFVNQRPVDSRTLNYALIESYHESIPKGRYPLAFVFFECD
                     PAAVDVNVHPAKREVRFRSEPSVRGFVIRAVLQRLREIGPSSSAVRNDEIRTTNVAVA
                     PPIATAATAPSTSGWTGTPFDGAHNIRPAANPTETPPPREAASMPAVASPVMRQSSPP
                     PTVAVGPAEPVTPNWRFVGLAHGSYALFETSSGLVLLDRRAAHERVWFERLQESFRSG
                     DVPSQR"
BASE COUNT          177 a          398 c          286 g          165 t
ORIGIN      
        1 cgctggctcg gcacatcgcc cgaccgaaac gattcctgca agcgctcgaa ccacacgcgc
       61 tcatgcgccg cacgtcgatc gagcaacacc agtcccgaac tcgtttcgaa cagcgcgtag
      121 ctgccatgcg ccagcccgac gaaacgccag ttcggtgtga ccggctccgc cggcccgacc
      181 gcgaccgtgg gcggcggcga tgattgtcgc ataacgggtg acgcaaccgc gggcatgctg
      241 gcggcctccc gcggcggcgg cgtctccgtc gggttcgccg ctggacggat gttgtgcgct
      301 ccgtcaaacg gcgtccccgt ccagccgctc gtcgagggtg ccgttgccgc cgtcgcgatt
      361 ggcggcgcca ccgcgacgtt cgtcgtccga atctcgtcat tccgcaccgc cgacgacgac
      421 ggcccgatct cgcgcaaccg ctgcaacacc gcgcgtatca caaatcccct tacgctcggc
      481 tcactccgaa agcgcacctc ccgcttcgcc gggtgcacgt tcacgtcgac cgccgccgga
      541 tcgcactcga aaaacacgaa cgccagcgga tagcgcccct tcggaatcga ttcgtgataa
      601 ctctcgatca gcgcgtaatt caacgtgcgg ctgtcgaccg gccgctggtt cacgaacaca
      661 atcatctcgt gccgcgtccc gcgcccgatg cctggtcgcc cgattaatcc gctcagccgc
      721 attcccgtct ccaccgcctc gagcggcacg agcgcctccg ccgtttgccg gccaaaaatc
      781 tccgcgatcc tctccgccag cgtcgcgcat tccggcgaac gaaagatcac ccgcccgtcc
      841 tcgatcagcg tgaatgccgt gcgcgggcac gccagcgcgt aaagccgcac gcactgcacg
      901 atgtgcgccg cctccgtctg atcggtcttc aaaaacttcc gccgcgccgg caccgagttg
      961 aacaaatgcg tcacctcgat ccgcgtcccc accggccggc cgcaatcccg cacatgcacg
     1021 aacttg
//
LOCUS       DASPLT010000245         1015 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_269109, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000245 DASPLT010000000
VERSION     DASPLT010000245.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1015)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1015)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1015
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_269109"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..506
                     /gene="rplD"
                     /locus_tag="VEA63_02610"
     CDS             <1..506
                     /gene="rplD"
                     /locus_tag="VEA63_02610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009512992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HYD82905.1"
                     /translation="RQGTHSTKTRGEVRGGGKKPWRQKGTGRARAGSIRSPLWVGGGV
                     VFGPKPRDYSKKVNAKVKRLAFSRALFDRATAGELAVIEQFDTKGTKTKAINTILGRI
                     APKGKVLLVDAPFAVETARASRNIERVSLQEAAKLNTLDLAQYAKIIVSTKALETILA
                     RVNGGKN"
     gene            506..787
                     /gene="rplW"
                     /locus_tag="VEA63_02615"
     CDS             506..787
                     /gene="rplW"
                     /locus_tag="VEA63_02615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HYD82906.1"
                     /translation="MQADKILKLVRLSEKSNKLSSELGQYTFEVFGHANKHQIAEAVE
                     QTFKVTVRRVNTQNYRGKNKRTRKGGATVGSDYKKAIVTLKAGDKIELV"
     gene            820..>1015
                     /gene="rplB"
                     /locus_tag="VEA63_02620"
     CDS             820..>1015
                     /gene="rplB"
                     /locus_tag="VEA63_02620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373052.1"
                     /note="one of the primary rRNA-binding proteins; required
                     for association of the 30S and 50S subunits to form the
                     70S ribosome, for tRNA binding and peptide bond formation;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HYD82907.1"
                     /translation="MAIHSFRPLTPAGRFTSLNKAEGLSKKRPERGLTQSKNKTGGRN
                     VYGRITTRHRGGGHKQLYRVI"
BASE COUNT          236 a          344 c          279 g          156 t
ORIGIN      
        1 accgacaagg cacgcactcg accaagaccc gcggcgaagt ccgtggcggt ggcaaaaagc
       61 cgtggcgcca gaagggcacc ggccgcgctc gcgcgggttc catccgctca cctttgtggg
      121 tcggtggtgg cgtcgtgttc ggcccgaagc cgcgtgacta ttcgaagaag gtcaacgcca
      181 aggtgaagcg gctcgcgttt agccgcgcgc tctttgatcg cgcgaccgcc ggcgagctcg
      241 ccgtcatcga gcagttcgac accaagggca ccaagaccaa ggcgatcaac accatcctcg
      301 gtcgcatcgc cccgaagggc aaagtgctcc tcgtcgacgc ccccttcgcc gtcgagaccg
      361 cgcgcgcctc gcgcaatatc gaacgcgttt ccctccagga agccgcgaag ctcaacactc
      421 tcgatctcgc ccaatacgcg aaaatcatcg tcagcaccaa ggcgcttgaa acgatccttg
      481 cccgcgtcaa cggaggtaaa aactaatgca agccgacaag atcctcaaac tcgtccgcct
      541 gtcggaaaaa tcgaacaagc tctcgtccga gctgggccaa tataccttcg aagtcttcgg
      601 ccacgccaac aagcaccaga tcgccgaagc cgttgaacag acgtttaagg tgacagttcg
      661 ccgcgtgaac acgcagaact accgcgggaa aaacaagcgc acccgcaagg gcggcgcgac
      721 cgtcggttcc gattataaga aggcaatcgt gacgcttaaa gccggcgaca agatcgagct
      781 cgtctaaagc gaactcgccc aacccggaga aactccacca tggcaatcca ttcctttcgt
      841 ccgctcacgc ccgccggccg cttcacctcg ctgaacaagg ccgagggtct ctcgaagaag
      901 cgcccggagc gcggcctcac ccagtcgaag aacaagaccg gtggccgtaa cgtttacggc
      961 cgcatcacca cgcgtcatcg cggcggcggt cacaagcagc tctaccgtgt tatcg
//
LOCUS       DASPLT010000246         1949 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_146711, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000246 DASPLT010000000
VERSION     DASPLT010000246.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1949)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1949)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1949
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_146711"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..396)
                     /locus_tag="VEA63_02625"
     CDS             complement(<1..396)
                     /locus_tag="VEA63_02625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD82908.1"
                     /translation="MAGAATSGRRRPGLRLTASQVEVDVFIGLAGVRWRNSRTETSRS
                     TFDCTLPSFRPLRLLFALFAAASLLAAAEPALTDVRVNTRSVPRYEKFELTFDARGDW
                     SNPFDPAQVAIDCRVEPPQGEPFVVPAFYF"
     gene            complement(396..1736)
                     /locus_tag="VEA63_02630"
     CDS             complement(396..1736)
                     /locus_tag="VEA63_02630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007361854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HYD82909.1"
                     /translation="MSKKRYVLVGTGVRARNFITPLVERFQSDGELVALCDLSPTRMA
                     YYNDQLASKLGYHAVPTYAAAQFDRMLKEQRADCVIVTTKDSTHHDYIVRALRQGCDV
                     ITEKPMTVDAEKTRVILDAVKETGRKVQVAFNYRWSAHRTKVRDLIASGVVGRVASVN
                     VEYLLNTNHGADYYRRWHATMADSGGLLVHKSTHHFDLVNWWIDAIPEQVFAYGRLDF
                     YGRANAVARGDAALTAYERYTGATTATKDPFALDLREHESLEGLYLKAEADSGYLRDR
                     NVFREGIDIYDNMSVLVRYRTGVQLNYSLVSFSPREGMRVSINGDRGRVEYNEFQPTH
                     IPNVNGAKEAPRAPEDQGSESIRVYPLFKPSYEIQVEKLPGGHDGSDPVLAEQMFSAK
                     PPVDPFSRKAGHEQGAASILIGIAANQSIVSNRPVLLSELAELNPSAKHLSELV"
BASE COUNT          366 a          595 c          638 g          350 t
ORIGIN      
        1 gaaataaaac gccggcacga cgaacggttc gccttgtggc ggctcgacgc ggcagtcgat
       61 cgcgacctgc gccggatcga acggattgct ccagtcgccg cgcgcatcga atgtcagttc
      121 gaatttttcg tagcgcggaa cggaacgcgt gttgacccgc acatccgtca acgccggttc
      181 cgccgccgcg agcaacgatg cggctgcgaa cagcgcgaag agcaaacgca gcggacggaa
      241 gctcggcagc gtgcagtcga aggtggagcg cgacgtctcc gtccggctgt tacgccaacg
      301 cacgccggcc agcccgataa agacatcgac ctccacctgg ctcgcggtga gccgcaggcc
      361 aggtcgcctt cgaccgctcg ttgccgcccc agccatcaga caagttcgct gaggtgcttt
      421 gcgctcgggt tgagctccgc caactccgac agcagcacgg gccggttgct cacgatcgat
      481 tgattggcag cgataccgat gagaatcgaa gcggcgccct gttcgtggcc ggccttgcgg
      541 ctgaagggat cgaccggcgg tttggcggaa aacatctgtt ccgccagcac cggatcggat
      601 ccgtcgtggc cgccgggcag cttctcgacc tgaatctcgt agctcggctt gaatagagga
      661 tatacgcgga tcgactcgct tccctggtcc tcgggggcgc gaggcgcttc cttggcaccg
      721 ttgacgttcg gaatgtgcgt cggttgaaac tcgttgtatt cgacgcggcc acggtcgccg
      781 ttgatcgaaa cgcgcatgcc ctcgcgcggc gaaaaactga cgagcgaata attgagctgc
      841 acgccggtgc gatagcgaac gagcacggac atgttgtcgt agatgtcgat gccttcgcga
      901 aacacgttgc gatcgcggag atagccgctg tcggcctcgg ccttgagata caagccctcg
      961 agactctcgt gctcgcgcag atcgagcgcg aacggatcct tcgtcgccgt ggtggcgccg
     1021 gtgtagcgct cgtaggcggt cagcgcggcg tcgccgcggg ccacggcatt ggcgcggccg
     1081 taaaagtcga gccggccgta ggcgaagacc tgctcgggaa tcgcgtcgat ccaccagttg
     1141 acgaggtcga aatgatgcgt gcttttgtgc acgagcaggc cgcccgaatc ggccatcgtg
     1201 gcgtgccagc gacggtagta atcggcgccg tggttcgtgt tgaggaggta ttcgacgttc
     1261 acactggcga cgcggccgac gacgccggaa gcaatgaggt cgcgcacctt cgtgcgatgc
     1321 gcggaccagc ggtaattgaa cgcgacctgg accttgcggc cggtttcctt gacggcgtcg
     1381 aggatcacgc gcgtcttctc cgcgtcgacg gtcatcggct tttccgtgat cacgtcgcag
     1441 ccctggcgca acgcgcgcac gatgtagtcg tgatgcgtcg agtccttggt cgtgacgatc
     1501 acgcaatcgg cgcgctgctc tttcagcatc cggtcgaact gcgcggccgc gtaggtcggc
     1561 acggcgtggt agcccaattt gctcgcaagc tggtcgttgt aatacgccat gcgcgtgggg
     1621 ctcagatcgc acagcgcgac gagttcgccg tcgctttgaa agcgctcgac cagcggcgtg
     1681 ataaagttgc gcgcccgcac tccggtgccg acgagaacat aacgtttttt gctcataaga
     1741 aaaaagtaac gggatgggtg atgtcgcgac ggagccggct agcggctgcc gccttgaaaa
     1801 cggatgcgcg gatcgagcca gagcaggagc aggtcgctca cgagcgtgcc gagcacgccg
     1861 agcacgctca tcaccatcag catcgagccc gcgagataaa tgtcttccga caacagcgcg
     1921 gtcagcatca ccggaccgac cagcggcag
//
LOCUS       DASPLT010000247         1265 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_208348, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000247 DASPLT010000000
VERSION     DASPLT010000247.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1265)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1265)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1265
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_208348"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..278)
                     /locus_tag="VEA63_02635"
     CDS             complement(<1..278)
                     /locus_tag="VEA63_02635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight protein arginine
                     phosphatase"
                     /protein_id="HYD82910.1"
                     /translation="MSIRPLVSMADTILVVCTANICRSPMAAGLLRHALAGQPEPLRS
                     LKVISAGVAARHGERVSENSVIALRKVGIDVSQHTSQPLTQDMLNEAAA"
     gene            complement(614..>1265)
                     /locus_tag="VEA63_02640"
     CDS             complement(614..>1265)
                     /locus_tag="VEA63_02640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD82911.1"
                     /translation="ALLFLVFVGAIHATPLQHDLGHNLAYHRAHTLPDDLPAATAKAQ
                     PLVLDLRFTIAGDGAAQALAIWLRSHATRATPVIVLVNAETPRALHPVFAALTSQTGL
                     LTIGSPASGFIPDISLAIPLEAERRAYQALEQGVSLEMLLRENTDKPRRDEAAIMRER
                     DNPPEEIPEEELTDEELTENPASAKETKTAAPPPLIDKALQRAVQLHRALLALKRL"
BASE COUNT          224 a          327 c          493 g          221 t
ORIGIN      
        1 gcggcggcct cgttgagcat gtcctgcgtc agcggctggc tggtgtgttg cgagacatcg
       61 atgccgactt tgcggagggc gatgaccgag ttctccgaaa cgcgctcgcc atggcgggcg
      121 gcgacgccgg cagaaataac cttgagcgaa cggagtggct ccggctggcc ggcgagggca
      181 tggcgcaaca agcctgcggc catcgggctg cggcaaatgt tcgcggtgca aacgactagg
      241 atcgtgtcgg ccatggaaac gaggggtcga atggacacga aaaccggggg attgcaaaga
      301 gcggattcgg gccgctcgcc gcttcaggcc gcgtggccgg atcgcagtgg aagaaacgct
      361 aacgctcgcg cacgtgtgcg atcagatgtc gcatatgcga catctaactg cacgccgtag
      421 ttgagagctt ggacatgaga aaaccgcggc ggagcggaga aacgttcctc tggcccggct
      481 gggcgcccgg gtggcggcac gtttaaccgc ctacgcgtcg gggagcggag ggcggaaaga
      541 tgtcgcatat gcgacatcgg aacgcatgtc gcggggcgga cgagggcgaa gggtgcccga
      601 caggggatag gggttagagc cgtttcaagg cgagcagcgc gcggtggagt tggacagcgc
      661 gttgcagggc tttgtcgatc agcggcggcg gagcggcggt cttcgtttcc ttcgcagacg
      721 cggggttttc ggtgagctct tcgtcggtga gttcttcctc cggaatttct tccgggggat
      781 tgtcgcgctc gcgcatgatc gcggcttcgt cccggcgtgg cttgtcggtg ttttcgcgca
      841 ggagcatctc gagtgagacg ccttgttcga gtgcctgata ggcacggcgc tctgcttcca
      901 acggtatcgc gagtgagata tccgggatga agccggaggc gggcgagccg atcgtgagga
      961 ggccggtttg cgaggtcagc gcagcgaaga ctgggtgcag ggcgcgcggg gtctcggcgt
     1021 tgacgagcac gatgacaggc gtggcgcgag tggcgtgcga acggagccag attgcgagcg
     1081 cttgggcggc gccgtcgccg gcgatcgtga agcgaagatc gagcacgagc ggttgcgctt
     1141 tcgccgtcgc ggctggcaaa tcgtcgggca aggtgtgagc gcggtgatag gcgagattgt
     1201 gcccgaggtc gtgttggagc ggcgtggcgt gaatcgcgcc gacgaaaacg agaaacagga
     1261 gcgcg
//
LOCUS       DASPLT010000248         1188 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_269168, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000248 DASPLT010000000
VERSION     DASPLT010000248.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1188)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1188)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1188
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_269168"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..181
                     /locus_tag="VEA63_02645"
     CDS             <1..181
                     /locus_tag="VEA63_02645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="rRNA large subunit methyltransferase I"
                     /protein_id="HYD82912.1"
                     /translation="YTCSHHMHDAELRSVLAEAAADAKRKVRVLEFCHQPADHPVLLS
                     MPESEYLRGYVVRVE"
     gene            complement(262..633)
                     /gene="raiA"
                     /locus_tag="VEA63_02650"
     CDS             complement(262..633)
                     /gene="raiA"
                     /locus_tag="VEA63_02650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HYD82913.1"
                     /translation="MNRQNSHELIVSGIHLELTPSLKTFVREKAERLFRHEDRIVRIR
                     VELECDRNEAIAHRFKAKGHIEIQGPDMNAVVQADECHKAVSLLIDKLDRMLGKRHDL
                     HKVRRHQIDPVSEHPALLKAV"
     gene            709..>1188
                     /locus_tag="VEA63_02655"
     CDS             709..>1188
                     /locus_tag="VEA63_02655"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HYD82914.1"
                     /translation="MSSPREALQTFAAVVVTGGSSGIGKSFIELCAKLVPELRFCNLS
                     RSVPAINVDQLNLRHVACDLGDAAALERGAEDVLAWLTRDVPAGRLLLINNSGFGSYG
                     CFPEHNLTHQLELLDVNVRAVVELTGRLLPELKRRGGVVINVASTAAFQPTPFMATYG
                     "
BASE COUNT          207 a          317 c          394 g          270 t
ORIGIN      
        1 gtatacgtgt tcgcatcaca tgcacgatgc ggagttgcga agcgtgctcg cggaggcggc
       61 ggcggacgcg aagcgaaagg tgcgtgtgct ggagttttgt catcagcccg cggatcaccc
      121 ggtgttgctg tcgatgcccg agagcgagta tttgcgcggt tacgtggtgc gcgtggagtg
      181 acgcgtccga tggatcgcgc cgaaaaaaag cccgtcggcg cgggccggcg ggcgtgaacg
      241 ggacgtgtcg ttgaaaaaca actacacggc tttgagcagg gcgggatgct cggagaccgg
      301 gtcgatctgg tggcggcgga ccttgtgcag gtcgtggcgc ttgccgagca tgcggtcgag
      361 tttgtcgatg agcagggaaa cggccttgtg gcattcgtcc gcctggacga cggcgttcat
      421 atcgggtcct tgaatttcga tgtgcccctt ggctttgaac cggtgagcga tcgcttcgtt
      481 gcggtcgcat tccagctcca cgcggatccg cacgatgcgg tcttcgtgac gaaacaatcg
      541 ctccgctttc tctcgaacaa aagttttcag ggacggtgtc agttcgaggt gaattccgga
      601 gacgatgagt tcgtggctgt tttgtctgtt catgatactg cgttatgttt gggtttcacc
      661 gagccggcgc ggaccaccca cgccggccga aaatcatccc tcgaatttgt gtcctcgcca
      721 cgcgaagcgc tccaaacttt cgccgccgtc gtcgtgaccg ggggatcttc cggtattgga
      781 aaatcgttca tcgagctgtg tgcgaaactg gttccggagc tgcgattttg caacctgtct
      841 cgctcggttc cggccataaa tgtagaccag cttaacttgc gccatgtggc gtgcgatctt
      901 ggcgacgcag cggcactcga acgtggggcg gaggacgttc tcgcctggtt aactcgcgac
      961 gtgccagcgg ggcggctgct gctcatcaat aacagtggct tcggctcgta tggctgcttt
     1021 cctgagcaca acttaactca ccagctcgaa ctattagatg ttaacgtgcg cgcagtggtg
     1081 gaactcacgg ggcggttgct gccggagctg aagcggcgcg gcggtgtggt gatcaatgta
     1141 gcgtcgaccg cggcgtttca gcccacgccg ttcatggcga cgtatggc
//
LOCUS       DASPLT010000249         2540 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_227977, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000249 DASPLT010000000
VERSION     DASPLT010000249.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 2540)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2540)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2540
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_227977"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..136)
                     /locus_tag="VEA63_02660"
     CDS             complement(<1..136)
                     /locus_tag="VEA63_02660"
                     /EC_number="1.1.1.42"
                     /inference="COORDINATES: protein motif:HMM:NF015904.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HYD82915.1"
                     /translation="MSAKPTIIYTKTDEAPALATYSLLPIVQAFTKHADINVETRDIS
                     L"
     gene            295..729
                     /locus_tag="VEA63_02665"
     CDS             295..729
                     /locus_tag="VEA63_02665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HYD82916.1"
                     /translation="MNYAKVAPATMQAMAALQAAVARTGLDLNLRHLVTLRASQINGC
                     AFCIDMHVRDAKKAGETDERLYLLDAWPEVDLYTARERAALQWTEGLTKLAGGHVSEE
                     DFAAARAEFSEEELAHLTLAIVTINGWNRFNVGFRTPPGFGG"
     gene            762..1697
                     /locus_tag="VEA63_02670"
     CDS             762..1697
                     /locus_tag="VEA63_02670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009509111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma-70 factor"
                     /protein_id="HYD82917.1"
                     /translation="MKENPAYRHADVFTEHRALLFGIAYRMLGSVAEAEDLVQETYLR
                     WQRQDAAEIETPKAWLVATLTRLGIDQLRSARHRREEYVGVWLPEPIVDDAAPAPDRA
                     AALADSLGVAFLFLLETLAPVERAVFLLREVFDYDYAEIAGIVGKTEANCRQMVSRAK
                     ATLAQREATSTAPTVKAEQIVRRFMGACATGAVGELLAVLTDDAVLYTDGGGRVKSAL
                     RPIRNADRISRFFVGIRERAMVGAEVSFTRVNGDVGVLVRRRDGAVSVSAFAFAGDRI
                     RAIYMINNPDKLRHVIGERLEAEQKPRMDTNEHES"
     gene            complement(1775..>2540)
                     /locus_tag="VEA63_02675"
     CDS             complement(1775..>2540)
                     /locus_tag="VEA63_02675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HYD82918.1"
                     /translation="IWLAAMTAPTLFPSVPASFVVAPLTFVQHLQLLLKSMIVASSWT
                     LSYFALKHLPVTLVSPIRATGPVWTLLGALILLAERPSWMESLGVVITLASFFGLSAA
                     GAREGIHFGTNRWIWLLIAGTLLNAVSALYDKFLLGQQGFSAATVQAWFSIYLTLLFL
                     PLAVGWKLRWWPRHEFHWRWSIVFMSLALLIADFVYFNALRDPDALISLVSSFRRGST
                     LVAFAGGVLLFNEPNWRQKLPAVIGVLIGIVLTILG"
BASE COUNT          500 a          680 c          915 g          445 t
ORIGIN      
        1 cgagggagat gtcgcgcgtt tcgacgttga tgtccgcgtg cttcgtgaag gcctgcacga
       61 tcggcaggag cgaataggtc gcgagcgcgg gagcttcgtc ggtcttggtg tagatgatgg
      121 tcggcttggc ggacatagag ataaaaggga cggaatgaac ggtccacgta agcggcggga
      181 ggacggacgg tcaaaagcga attgcggggt tgctgtcaca aatcgaaagg ctgtttcgtc
      241 ccagagtctg ctatgacgaa ctcaactctc gaaaaaacga aggctcaacc gcgcttgaac
      301 tacgcgaaag tcgcgccggc gacgatgcag gcgatggccg cattgcaggc cgccgtcgcg
      361 cgcacgggcc tcgatctgaa tttgcgccat ttggtgacgc tgcgcgcctc gcagatcaac
      421 ggctgcgcgt tctgcatcga catgcatgtg cgcgacgcga agaaggcggg cgaaaccgac
      481 gagcggcttt acctgctcga tgcgtggccc gaggtcgacc tctacacggc gcgggagcgc
      541 gccgcgctgc agtggacgga agggttgacg aagctggcgg gcgggcatgt gagcgaggag
      601 gactttgcgg cggcccgcgc ggaattttcc gaagaagagc tcgcgcatct cacgctggcg
      661 atcgtgacga tcaacggctg gaaccggttc aacgttggct ttcgcacgcc gccgggtttc
      721 ggcggttgag ggaacgcttg gttctgaacg acgcttccgc catgaaagag aatcccgctt
      781 atcgccacgc ggacgtgttc actgaacatc gcgcgctgtt gttcggcatc gcttatcgga
      841 tgctcggcag cgtcgcggag gcggaggacc ttgtgcagga aacctatttg cgctggcaac
      901 ggcaggacgc ggcggagatc gagacgccga aggcgtggct cgtggccacg ctcacgcggc
      961 ttggcatcga tcaattgcgt tcggcgcgtc accggcgcga ggagtatgtc ggcgtgtggc
     1021 tgccggagcc gatcgtcgac gacgccgcgc cggcgccaga tcgcgcggcg gcgctcgcgg
     1081 attcattggg cgtggcgttt ctatttttgc tggaaacgct cgcgccggtg gagcgcgcgg
     1141 tgtttttgct gcgtgaagtg ttcgactacg actatgcgga gatcgcgggg atcgtcggaa
     1201 aaaccgaggc gaactgtcgc cagatggtca gccgcgcgaa agcaactctc gcgcagcgcg
     1261 aggcgacatc cacggcgccg accgtgaagg ccgaacagat cgtgcggcgg ttcatgggtg
     1321 cgtgcgcgac cggggcggtg ggcgagttgc tcgccgtgct caccgatgat gcggtgctct
     1381 atacggatgg aggcggtcgg gtgaaatcgg cgctgcggcc gatccggaat gcagatcgca
     1441 tcagccgttt cttcgtcggc attcgcgagc gggcgatggt gggagcggaa gtgtcgttca
     1501 cgcgcgtcaa cggcgatgtg ggcgtgctcg tccgtcgccg cgacggcgcg gtttcggtga
     1561 gcgcgttcgc gtttgcgggc gatcggattc gcgcgatcta catgatcaac aatcccgaca
     1621 agctgcggca tgtgatcggc gagcggcttg aggcggaaca gaaaccacga atggacacga
     1681 atgaacacga atcctgaaga ttcgagagac gcgtcgtgtg ctgttttcat tcgtgtcgat
     1741 tcgtatccat tcgtggttga gatggatttt cggtctagcc gaggatggtg agcacgatgc
     1801 cgatgagcac gccgatgacg gcggggagtt tttggcgcca gttgggttcg ttgaacaaca
     1861 gcacgccgcc ggcgaaggcg acgagcgtgc tgccgcggcg gaaactcgac acgagcgaga
     1921 tcaatgcgtc gggatcgcgg agcgcgttga agtagacgaa gtcggcgatg agcagcgcga
     1981 gcgacatgaa gacgatgctc cagcgccagt ggaattcatg gcgcggccac cagcggagtt
     2041 tccagccgac ggcgagcgga agaaacagca gcgtgagata tattgaaaac cacgcctgca
     2101 ccgtcgcggc ggagaaaccc tgttggccga gcagaaactt gtcgtagagc gcgctgacgg
     2161 cgttgagcag ggtgccggcg atgagcagcc agatccagcg gttcgtgcca aagtggattc
     2221 cttcgcgcgc gcccgcggcg gagaggccga agaacgaggc gagggtgatc acaacaccga
     2281 ggctttccat ccacgagggg cgttcggcga ggaggatcaa cgcaccgagg agcgtccaga
     2341 cgggaccggt ggcacgaatc ggcgacacga gcgtgacggg caggtgtttt agcgcgaaat
     2401 agctgagtgt ccacgagctc gcgacgatca tggatttcag cagcaattgg aggtgctgca
     2461 cgaacgtcag cggcgcgacg acgaagctgg cgggaacgct ggggaaaagc gtcggcgctg
     2521 tcatggccgc gagccagatc
//
LOCUS       DASPLT010000250         1091 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_25516, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000250 DASPLT010000000
VERSION     DASPLT010000250.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1091)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1091)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1091
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_25516"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..438)
                     /locus_tag="VEA63_02680"
     CDS             complement(<1..438)
                     /locus_tag="VEA63_02680"
                     /inference="COORDINATES: protein motif:HMM:NF014456.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD82919.1"
                     /translation="MSNAAPQFSISLANPRGFCAGVDRAIDIVNRALDTFGAPIYVRH
                     EVVHNKFVVENLKQRGAIFVDELKDVPHAAPGEPVPIVVFSAHGVSQAVKAEADALGL
                     KVFDATCPLVTKVHMEVIQYSRAGHECILIGHAFHPEVEGTMGQ"
     gene            complement(431..982)
                     /locus_tag="VEA63_02685"
     CDS             complement(431..982)
                     /locus_tag="VEA63_02685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003094726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HYD82920.1"
                     /translation="MNAVMSDDVRGKIAVRDLDRATAAQFAPGVIGPGAQVTLHFALA
                     IAGGARDGEVIDSNFDRAPVTFTVGDGSLLPGFEQALFGLAAGERREIVLEADAAFGP
                     VNDDNLQRFAPYQFPPDLPLATGLMVEFGDAAGNTQAGVVRNVGKQWVEVDFNHPLAG
                     RAIRFSVHVHDVAQPAPQVTAHE"
BASE COUNT          201 a          359 c          334 g          197 t
ORIGIN      
        1 ctggcccatc gtgccttcca cttccggatg gaacgcgtgc ccgatcagga tgcactcatg
       61 tcccgctctg ctgtactgga tgacttccat gtgaaccttc gtcacgagcg gacacgtggc
      121 atcgaaaact ttcagaccca gcgcgtcggc ctcggctttc accgcctgcg acacgccgtg
      181 cgcgctgaac acgacgatcg gcaccggctc gcccggcgcg gcgtgcggca catccttcaa
      241 ctcgtcgacg aagatcgcgc cgcgctgctt caggttttca acgacgaact tgttgtgcac
      301 cacttcgtgc cgcacataga tcggcgcgcc gaacgtatcg agcgcgcggt tgacgatgtc
      361 gatggcgcga tcgacaccgg cgcagaaacc gcgcggattc gcgagactga tcgagaattg
      421 cggtgcggcg ttactcatga gctgttacct gcggtgctgg ctgtgcaacg tcatgcacat
      481 gcacgctgaa gcgaatggcg cgccccgcga gcggatgatt gaagtctacc tccacccatt
      541 gcttgccgac gttgcggacg acgccggcct gcgtgttgcc ggcggcgtcg ccgaactcga
      601 ccatcagtcc cgtagccaat gggagatccg gcgggaactg gtacggggca aaccgttgca
      661 ggttgtcgtc gttcaccggt ccaaacgccg cgtcagcctc cagcacaatc tcgcggcgtt
      721 cgccggcggc gagaccgaac agggcctgtt cgaagcccgg cagcaagctg ccgtcaccga
      781 cggtgaacgt aacaggcgca cggtcgaagt tgctgtcgat cacttcgcca tcgcgcgcgc
      841 ctccggcgat tgccagcgcg aaatgcagcg taacctgcgc gccggggccg atcacgccgg
      901 gcgcgaactg cgctgccgtg gcgcggtcga gatcgcgcac ggcaattttt ccgcgcacgt
      961 cgtcactcat gacggcgttc acctttcaat atgtcgagca cgatcaggaa cgcgccgacg
     1021 ttcagcgcca cgtcggcgat gttgaacgtc gcgaagcgcc agtcgccgta atacacgctg
     1081 atgaaatcga c
//
LOCUS       DASPLT010000251         1137 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_228016, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000251 DASPLT010000000
VERSION     DASPLT010000251.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1137)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1137)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1137
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_228016"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1128)
                     /locus_tag="VEA63_02690"
     CDS             complement(<1..1128)
                     /locus_tag="VEA63_02690"
                     /inference="COORDINATES: protein motif:HMM:NF013707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HYD82921.1"
                     /translation="MIAHVASYRSRVPFIHFFDGFRTSHEVQKLRMLSDAELHAVIDE
                     KDIAAFRARAMTPDRPTLRGTAQNPDVYFQGREAVNPFYRDLPGTVQDTMERFAAVTG
                     RRYRLFDYYGHPEAERVVVAMGSSVETLAETAEWLAERGEKVGVLAVRLFLPFDVKAF
                     LAALPTTVKAIGVLDRTKEPGSIGEPLYLNVLAALAAGRSPLAGSARVVGGRYGLGSK
                     EFTPGMAKAVFENLGQAEPRNHFTVGILDDVTGTSLPFDVDFDLEAAEVRRCVFVGLG
                     ADGTVGANKNSIKIIGEQTTNFAQGYFVYDSKKSGSMTTSHLRFGPRPIRAPYLIRRA
                     GFVACSQFSFVGKTDVLGYAAPGATVLLNSPYAADETWRTLP"
BASE COUNT          216 a          447 c          291 g          183 t
ORIGIN      
        1 cggcagcgtc cgccacgtct cgtccgccgc atacggcgag ttcaacagca ccgtcgcccc
       61 cggcgccgcg taacccagca cgtccgtctt ccccacaaaa ctaaactggc tgcacgccac
      121 aaagcctgcc cgccggatca aatacggcgc ccgaatcggg cgtggcccaa accgcaagtg
      181 cgacgtcgtc atcgagcccg acttcttcga gtcgtaaacg aagtagccct gcgcaaagtt
      241 cgtcgtctgc tcgccgatga tcttgatcga gttcttgttc gctccgaccg tcccgtccgc
      301 gcccagcccc acgaacacgc accgccgcac ctccgccgct tccagatcga aatccacgtc
      361 gaacggcagc gacgtccccg tcacgtcgtc gagaatcccc accgtgaaat gattccgcgg
      421 ctcggcctgt cccagatttt cgaataccgc cttcgccatc cccggcgtga attccttcga
      481 ccccagcccg tatcgcccgc caaccacgcg cgctgagccc gcgagtggcg aacgccctgc
      541 ggcgagcgca gccagcacat tgagataaag cggctcgccg atggatcccg gctccttcgt
      601 ccgatcgagg acgccgatcg ccttcaccgt cgtcggcaac gccgccagga acgccttcac
      661 gtcgaacggc agaaacaacc gcaccgcgag aacacccacc ttttcccccc gctccgccag
      721 ccactccgcc gtttccgcga gcgtttccac actgctcccc atcgccacca ccacgcgctc
      781 ggcttcggga tgcccgtagt agtcgaaaag ccgataccgc cgccccgtca ccgccgcgaa
      841 ccgctccatc gtgtcctgca cggtgcccgg cagatcgcga tagaacggat tcaccgcctc
      901 gcggccctga aaatacacgt ccggattctg cgccgtgccg cgcagcgtcg gccggtccgg
      961 cgtcatcgcc cgcgcgcgaa acgccgcgat gtccttttcg tcaatcaccg cgtgcaactc
     1021 cgcgtcagac aacatccgca gcttttgcac ttcgtgcgag gtgcgaaacc cgtcgaagaa
     1081 atggatgaac ggcacccgcg atcgatagct cgccacgtgc gcgatcaacg ccagatc
//
LOCUS       DASPLT010000252         1136 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_85294, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000252 DASPLT010000000
VERSION     DASPLT010000252.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1136)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1136)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1136
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_85294"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..400
                     /locus_tag="VEA63_02695"
     CDS             <1..400
                     /locus_tag="VEA63_02695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine diphosphorylase"
                     /protein_id="HYD82922.1"
                     /translation="VIVGERCVLGNASEFKNCLLMDRVEVPHFSYVGDSVLGNRAHLG
                     AGVICSNLRLDQRAIVVQTESAVHETGLRKFGAILGDEAQVGSNAVLQPGTVLGKRAL
                     VMPLTAFGGVLPANTIARSRQAITTIARRD"
     gene            476..>1136
                     /gene="trpS"
                     /locus_tag="VEA63_02700"
     CDS             476..>1136
                     /gene="trpS"
                     /locus_tag="VEA63_02700"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009511994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HYD82923.1"
                     /translation="MRTLTGIQPSGTLHLGNYLGAMRPAIEAQARGDCFYFIADYHSM
                     TSLFDPGERRKNTLNVALDWLACGMDPAKSVFWRQSDVSEVTELMWLLGSLTPMGLLE
                     RAHSFKDKTAKGISPNFGLFAYPVLMAADILLYDTNVVPVGKDQQQHLEMTRDIAIKF
                     NQTYGETFVVPEADIREELAVVPGLDGQKMSKSYGNTIDLFGEEKATRKKIMGIVMDS
                     RT"
BASE COUNT          227 a          332 c          361 g          216 t
ORIGIN      
        1 cgttatcgtc ggtgaacggt gcgtgctggg gaacgcgtcg gagttcaaaa actgtctgct
       61 gatggatcgc gtcgaagtgc cgcacttcag ctatgttggc gactcggtgc tcggaaaccg
      121 cgcgcatctg ggtgccggcg tgatctgttc gaatctacgg ctcgatcaac gcgcgatcgt
      181 ggtgcaaacg gagagcgcgg tgcacgaaac gggattgcgg aaattcggcg cgatcctcgg
      241 cgacgaggcg caggtcggca gcaatgccgt gctgcagccg ggcacggtgc tcggcaaacg
      301 tgcgctggtg atgccgctga cggcgttcgg cggcgtattg ccagcgaaca cgatcgcgcg
      361 gagccggcag gcgatcacga cgatcgcgcg ccgcgactga aaaaatcgtt ctcgttctcg
      421 cccggcttct cgttctctct ttccgactcc gcaaaaagag cacgactcgt ttcccatgcg
      481 caccctcacc ggtatccaac cttcgggcac gctgcacctc ggcaattatc tcggcgcgat
      541 gcgcccggcg atcgaggcgc aggcgcgcgg tgactgtttt tacttcatcg cagattatca
      601 ttcgatgacg tcgctgttcg atcccggcga gcggcggaag aacacgttga acgtcgcgct
      661 cgactggctc gcgtgcggaa tggatccggc gaaatccgtg ttctggcgcc agagcgacgt
      721 gtcggaagtc accgaactca tgtggctgct tggctcgctc acgccgatgg ggctgctcga
      781 gcgcgcgcac agtttcaagg acaagaccgc gaaaggcatt tcgccgaact tcggtctttt
      841 cgcatatccg gtgctcatgg cggcggacat cctgctctat gacaccaacg tcgtgccggt
      901 gggaaaagac cagcagcagc acctcgagat gacgcgcgat attgcgatca agttcaatca
      961 gacctacggc gagaccttcg tcgtgccgga agctgacatc cgcgaagagc tggccgtcgt
     1021 gccgggtctc gacgggcaga aaatgagcaa gagctacggc aacacgatcg atctcttcgg
     1081 cgaggagaaa gcgacgcgga agaaaatcat gggcatcgtg atggattcac gcacgc
//
LOCUS       DASPLT010000253         2733 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_3450, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000253 DASPLT010000000
VERSION     DASPLT010000253.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 2733)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2733)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2733
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3450"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..375)
                     /locus_tag="VEA63_02705"
     CDS             complement(<1..375)
                     /locus_tag="VEA63_02705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009513642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HYD82924.1"
                     /translation="MKILITGGSGFIGSHLAEHFQGKAHVRVLDNLRTGHRRNLAGLD
                     VEFIEGSITDRAIVRRAVEDVDYVFHLAALVSVPESVERPHECVDINVTGLLNVLEAS
                     AAAGVRKLCLSSSAAIYGNNPTV"
     gene            complement(372..1499)
                     /locus_tag="VEA63_02710"
     CDS             complement(372..1499)
                     /locus_tag="VEA63_02710"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009513643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HYD82925.1"
                     /translation="MSISTTSAPAVDLAAVSRAFPLLGRFRSGEPHGRGHINDTFAVT
                     LDQAGTAVRYIFQRINHRIFKDVPALMENISRVTAHATLKSTERGDADSRRALTLVPA
                     RDGKPYHRDADGGWWRCYLFIEGARTHDLIENPQQAREAARAFGEFQQLLVDLPGGRL
                     HETIPDFHHTRRRFETLQRAIAADAHGRAAQVAEEIAFVRSRESLVDCLLDLLKRGEL
                     PERITHNDTKLNNVMIDDATQTGVCVIDLDTVMPGLALYDFGDMVRSATNSAEEDERD
                     LSRVSARLPIFEALVEGYLAAARSFLNDAEISHLATSGRLITLEIGMRFLTDFLEGDV
                     YFKTRRPGHNLDRARNQFAMVRSMEAQAAEMEAIVKKHAAK"
     gene            complement(1496..2392)
                     /locus_tag="VEA63_02715"
     CDS             complement(1496..2392)
                     /locus_tag="VEA63_02715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HYD82926.1"
                     /translation="MQLSLVILAAGMGSRYGGLKQLDGVGPGGETLLDYAVHDAVRAG
                     FTRVVFIIRQAFEAEFRAQVGARYAGVIQVDYVFQELDALPAGFTAPAERTKPWGTGH
                     AVWCARSAIVGPFAVVNADDFYGRDSYVQLARFLRATGGGGRPEVALVGFTLANTLSE
                     NGAVSRGVCAVRDDGLLRGIVEHTGIRREDVGSGRTFTGDEIVSMNCWAFTPAIFAGL
                     ERQFAEFLRNRAAEPKAEFYLPAAISELIATGAVDVQVLPTQASWFGVTYREDKPGVE
                     AAIGRLIAAGEYPATLNSGISS"
     gene            2491..>2733
                     /locus_tag="VEA63_02720"
     CDS             2491..>2733
                     /locus_tag="VEA63_02720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD82927.1"
                     /translation="MARPTPPRPRSRPIAGDDNLAAQLRRLVADAPNDQPLPTTRALA
                     ELFGVANTTVFRLLRGLAAAGEIWQHPTNGRYYSSEA"
BASE COUNT          504 a          930 c          816 g          483 t
ORIGIN      
        1 caccgtggga ttgttgccgt agatcgccgc cgaactgctc aggcagagct tgcgcacgcc
       61 cgccgcggcg gacgcctcga gcacgttgag caggccggtg acgttgatgt ccacgcattc
      121 gtgcggccgc tcgaccgact ccggcacgct gaccagcgcg gcgagatgga aaacatagtc
      181 cacgtcctca accgcgcgac gcacgatggc gcgatcggtg atcgagcctt cgataaactc
      241 cacgtcgagg cccgcgagat tcctccggtg accggtgcgc aaattgtcga gcacccgcac
      301 gtgcgccttg ccctggaagt gctccgccaa atggctgccg ataaaaccgg aaccgcctgt
      361 gatcagaatt ttcatttcgc ggcgtgcttc ttcacgatcg cttccatttc cgcggcctgc
      421 gcctccatgc tgcgcaccat cgcgaactgg tttcgcgcgc ggtccagatt gtggccgggc
      481 cggcgggtct tgaagtaaac gtcgccctcg aggaaatcgg tgagaaaacg catgccgatc
      541 tcgagtgtga tcaaccggcc cgacgtggcg aggtgagaga tctccgcatc gttcaagaac
      601 gatcgcgccg ctgcgaggta accttccacc agcgcttcga agatcggcag ccgtgcgctc
      661 acccgcgaga gatcgcgctc atcctcctcc gccgaattcg tggcggagcg caccatgtcg
      721 ccgaagtcgt agagggcgag accgggcatc accgtgtcga ggtcgatcac gcatactcct
      781 gtctgcgtgg cgtcgtcgat catcacgttg ttcagcttcg tgtcgttgtg cgtaatccgc
      841 tccggcagtt cgccacgctt caacagatcc agcagacaat cgaccagcga ttcgcgcgag
      901 cgaacgaaag cgatctcctc cgccacctgc gcggcgcgtc cgtgcgcgtc ggcggcgatc
      961 gcgcgttgca acgtctcgaa gcggcgtcgc gtgtgatgga aatccggaat cgtctcgtgc
     1021 aaccggccgc ccggcaggtc caccagcagt tgttgaaact cgccgaacgc gcgcgccgct
     1081 tccctcgcct gctgcggatt ctcgatcaga tcgtgcgtcc gcgcgccttc gataaagaga
     1141 taacaccgcc accagccgcc gtcggcgtcg cgatgatacg gttttccgtc gcgagcggga
     1201 acgagcgtga gcgcgcgacg cgaatcagcg tcgccgcgct cggtcgattt caacgtggcg
     1261 tgcgcggtga cacgcgaaat gttttccatc aacgccggga cgtccttgaa aatccggtga
     1321 ttgattcgct gaaaaatata ccgcaccgcc gtgcccgcct gatcgagagt caccgcaaag
     1381 gtgtcgttga tgtggccccg gccatgcggt tcgccggagc gaaagcgccc cagcagagga
     1441 aaagcgcggg agaccgcagc caaatcgacc gcaggggccg acgtcgtcga gatgctcatg
     1501 atgaaatgcc agagttaagc gtcgcgggat attcgcccgc cgcgatcagc cggccgatcg
     1561 ccgcctcgac gccgggcttg tcctcgcgat acgtgacacc gaaccacgac gcctgcgtcg
     1621 gaagcacttg aacgtccacc gcgccggtcg caatcaactc ggagatcgca gccggaagat
     1681 aaaattccgc cttcggctcg gccgcacggt tccgcagaaa ctccgcgaac tgccgttcca
     1741 acccggcgaa gatcgccggc gtgaacgccc agcagttcat cgagacgatt tcgtcacccg
     1801 tgaacgtgcg gccgctcccg acgtcctcac ggcggattcc ggtgtgttcg acaataccgc
     1861 ggagcagccc gtcgtcgcgg accgcgcaca cgccgcgcga caccgcgcca ttttccgaaa
     1921 gcgtgttcgc gagcgtgaag cccaccagcg cgacttccgg ccggcctcct ccacccgtag
     1981 cccgaaggaa gcgcgccagt tgcacgtagg aatcccgtcc gtaaaaatcg tcggcgttca
     2041 cgacggcgaa ggggccgacg atcgcgctgc gggcgcacca caccgcatgt ccggtgcccc
     2101 acggcttcgt cctttcggct ggcgctgtga aacccgccgg aagcgcgtcg agctcctgga
     2161 acacatagtc gacctgaatc acgcccgcat agcgcgcgcc gacctgcgcg cgaaactccg
     2221 cctcgaatgc ctggcgaatg atgaaaacga cccgcgtaaa acccgcgcgc accgcatcgt
     2281 gcaccgcata atcgaggagc gtttcgcccc ccgggccgac gccgtcgagc tgcttgagcc
     2341 cgccgtagcg gctgcccatg ccggccgcaa gaatcaccag gctgagttgc atgacttatc
     2401 gacaacactc attgactgtg gtgcaacaat atatttttgt tgcactcatt ttccttgcgt
     2461 tgcgtgtctc tcgcttttgt tccattcgac atggcccgtc ccaccccgcc tcgtccgcgt
     2521 tcccgtccga tcgccggcga cgataatctc gccgcgcagc tgcgccgcct cgtggccgac
     2581 gcgcccaacg accagccgtt gccgacgacg cgcgcgcttg cggaattgtt cggcgtggcg
     2641 aacaccacgg tgttccgtct gttgcgcggc ctcgcggctg cgggcgagat ttggcagcat
     2701 ccgacgaacg gacgttacta ctcctcggaa gcg
//
LOCUS       DASPLT010000254         3150 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_85320, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000254 DASPLT010000000
VERSION     DASPLT010000254.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 3150)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3150)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3150
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_85320"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1976
                     /locus_tag="VEA63_02725"
     CDS             <1..1976
                     /locus_tag="VEA63_02725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD82928.1"
                     /translation="ALKDEPAILFIEMINEPHHHANDFAGSVAYINALAQAVRDTGCE
                     KILFHNLSQDFRIAPAIAASNAQGFSFGWYPTALGARRTLTENYLRWVDRYTPLHDPA
                     IPPLPRIVYEFDSADMTAGYMYPAMVRAYREAGAQFAAMFAYDMLATAPYNLGWQTHY
                     LNLVHSPRKAVSAIIAAEAMRTLPRGQTYGDYPHNRHFGPFRVSYEDDTSELVTAEKF
                     LHANDTTTAPPNPATLQRIVGVGSSPVVRYDGLGSYFLDKLDDGHWRLEVYPDAVFVQ
                     DPFAQRLNHETVSSRLIWRTWPMQLDLPDLGPAFSIEPLNADNPHRATARNGMFSIRP
                     GVYLISRDGAPTPKLPERIGHLDLREFVCPSAPDLPAQIVPHLRTEYPSDQPLTLAVD
                     LVAAAAPRSAVVHLRSLASNKTHATPLRPQRSYRYTAEIAAALFSPGPVEIFVVFETA
                     DGEAITSSTWTAHVVEPNSPVVLLDPTRDANLWFTLRTATERPGGYVEPLPAESNGPD
                     AVRLHFPTREAASPHVISLPIKTRVADRGAGIREAIRLRVTARGTGRHLQLSLIDADG
                     RTWSAVVAPPATWQPVDLPLASFRPASALKLPQGYPGTWNRELLAPDSTVPASAIALE
                     KLEHLQISAAFVDEVAAPMGAFVDLASVEFLFD"
     gene            2004..3038
                     /locus_tag="VEA63_02730"
     CDS             2004..3038
                     /locus_tag="VEA63_02730"
                     /inference="COORDINATES: protein motif:HMM:NF028222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HYD82929.1"
                     /translation="MKALVLTAPSKLELQDVPAPLPAADEVLIRVRACGICGSDLHGW
                     DGSSGRRTPPLIMGHEAAGEIAAVGESVTTWQIGERVTFDSTIYCGECDYCRAGRINL
                     CENRRVVGVAPAEYKQHGAFAEFLSVPQRILHRLPDDLPFHHAAMVEPVSIALHAVSL
                     VTLPANSVAVVVGTGMIGLLVVQALRHAGAQHIVAIDREPMRLALARELGATLALRSD
                     QSDLPAELAKLKPHGADVVFEVVGIEPTVQLALQLVRRGGDVVLVGNLAPQIGGFPLQ
                     SVVTREIKLHGSCGSAGEYPLAIDLIARGVVRVEPLITAVAPLDDGAGWFDRLTSPTG
                     RHNLKVVLTP"
BASE COUNT          536 a         1233 c          797 g          584 t
ORIGIN      
        1 tcgcgctcaa ggacgaaccc gcgatcctct tcatcgagat gatcaacgag ccgcatcatc
       61 acgcgaacga tttcgccggc tccgtcgcct acatcaacgc gctcgcccaa gccgtccgcg
      121 acaccggttg cgaaaaaatc ctcttccaca atctcagtca ggatttccgc atcgcacccg
      181 cgatcgccgc ctcgaacgct caaggtttca gcttcggttg gtatcccacc gcgctcggcg
      241 cccgtcgcac gctcaccgaa aattatcttc gctgggtcga ccgctacacg ccgctccacg
      301 atcccgcgat cccaccgctg ccgcgcatcg tctacgaatt cgacagtgcc gacatgactg
      361 ccggctacat gtatcccgcg atggtccgcg cctatcgcga agccggcgcg cagttcgccg
      421 cgatgttcgc ctatgacatg ctcgccaccg cgccctacaa tctcgggtgg caaacgcact
      481 acctgaacct cgttcactcc ccgcgcaaag ccgtgagcgc catcatcgcc gccgaagcga
      541 tgcgcaccct tccccgcggt caaacctatg gcgactaccc gcacaaccgc cacttcggac
      601 cgtttcgcgt cagctacgag gacgacacaa gcgaactggt caccgccgaa aaatttctcc
      661 acgccaacga cacgaccacc gcgccgccga atcccgcaac cttgcagcga atcgtaggcg
      721 taggcagttc gcccgtcgtt cgctacgacg gtctcggcag ctactttctc gataagctcg
      781 acgacggtca ctggcggctc gaggtctatc ccgacgcggt cttcgtccag gatcctttcg
      841 ctcaacgcct gaaccacgaa accgtttcct cgcgcctcat ctggcgcacg tggccgatgc
      901 aactcgatct tcccgacctc ggccccgcct tctcgatcga gccactcaac gccgacaacc
      961 cgcaccgcgc caccgctcgc aacgggatgt tctctatccg tccgggagtg tatttgattt
     1021 ctcgtgacgg cgcgccgacc ccaaagcttc cagaacgaat cggccatctc gatcttcgcg
     1081 agttcgtgtg tccgtccgct cctgatttgc ctgcgcagat cgttcctcac ctccgcaccg
     1141 aatacccctc cgatcaaccg ctcacactcg ccgtcgatct cgtcgccgcc gctgcccctc
     1201 gctccgccgt cgtgcacctg cgctcgctcg cttccaacaa aacgcacgcg actccgctcc
     1261 ggccgcagcg cagttatcgt tacaccgccg agatcgctgc agcactattc tctcccggcc
     1321 ccgtcgagat tttcgtcgtc ttcgaaaccg ccgacggcga agcaatcact tcgtccacgt
     1381 ggacggcgca cgtcgtcgag cccaacagcc ccgtcgtcct cctcgatccg acacgcgacg
     1441 cgaatctctg gtttacgctg cgcaccgcga ccgagcgccc cggcggctac gtcgaaccgc
     1501 ttcccgcgga gagcaacggt cccgacgccg tgcggcttca cttccccacg cgcgaagccg
     1561 cctcgcccca tgtgatctcc cttcccatca aaacacgcgt cgccgatcgc ggcgccggca
     1621 ttcgcgaagc gattcgtttg cgcgtcaccg cccgcggcac cggccgccat ctccaacttt
     1681 cgctcatcga tgccgatgga cgcacatgga gcgccgtcgt tgcaccgcca gccacatggc
     1741 agccggtgga ccttccgctg gcttcgttcc gccccgcctc cgccttgaaa cttccgcaag
     1801 gttatcctgg cacttggaac cgggagttgc tcgcgcccga ttccacggtt cctgcatctg
     1861 cgattgcgct cgagaaactg gagcatctgc aaatctctgc cgctttcgtc gatgaagtcg
     1921 ccgccccgat gggggctttc gtcgatctcg catccgtcga gttcctgttc gattagtcgc
     1981 gagcttcttg tagagtgcgg ccgatgaaag ccctcgtcct caccgccccc tccaaactcg
     2041 aacttcagga cgttcccgct cccctgcccg ccgccgacga ggttctcatc cgcgtgcgcg
     2101 cctgcgggat ctgcggcagc gatctgcacg gttgggatgg ctccagcggc cgccgcacgc
     2161 cgccgctcat catgggccac gaagccgccg gtgaaatcgc cgccgtcggc gaaagcgtga
     2221 ccacctggca gatcggcgag cgcgtgacct tcgactccac gatttattgc ggcgaatgcg
     2281 actactgccg cgccggtcgc atcaacctct gcgaaaaccg ccgcgtcgtc ggcgtcgctc
     2341 ccgccgaata caaacaacac ggcgccttcg ccgagttcct ctccgttcct caacgcattc
     2401 tccaccgcct gcccgacgac ctgccgtttc accacgctgc gatggtcgag cccgtgtcga
     2461 tcgcgctgca cgccgtcagc ctcgtcacgc ttccagccaa cagcgtcgcc gtcgtcgtcg
     2521 gcaccggcat gattggtctt ctcgtcgtcc aagccctgcg tcacgccggc gcgcagcaca
     2581 tcgtcgccat cgatcgcgaa cccatgcgcc tcgccctcgc ccgcgaactc ggcgccaccc
     2641 tcgcgcttcg ctccgaccaa agcgatcttc ccgccgaact cgcgaagctg aaaccccacg
     2701 gcgccgacgt cgtattcgaa gtcgtcggta tcgaacccac cgtgcaactc gcacttcaac
     2761 tcgtccgccg cggcggcgac gttgtgctcg tcggcaatct cgcgccgcaa atcggcggat
     2821 ttccgctgca aagcgtcgtc acccgcgaga tcaaactcca cggcagctgc ggctccgccg
     2881 gcgaatatcc gctcgccatc gatctcatcg cgcgcggcgt cgtgcgcgtt gaacccctca
     2941 tcaccgccgt cgctccactc gacgacggcg ccggctggtt cgaccgcctc acctcgccga
     3001 ccggccgcca caacttgaag gtcgtcctca ccccctgagc gcgcgttccc ttttcgacct
     3061 taatggcgaa atcctcgtca ccgccgcgag ccacggcctc gctcgcttca tcgcgaccag
     3121 cattcgtccg catgaaagcc cacccaacac
//
LOCUS       DASPLT010000255         1343 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_25631, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000255 DASPLT010000000
VERSION     DASPLT010000255.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1343)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1343)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1343
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_25631"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1170)
                     /locus_tag="VEA63_02735"
     CDS             complement(<1..1170)
                     /locus_tag="VEA63_02735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012377127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HYD82930.1"
                     /translation="MKTSPRKNTGSLSRRDFCRTVAIAGAVFSVPTIIPSRLLGANSP
                     SNRFRIGQIGCGRIARGHDMPDVFKTGMADIVALCDVDSKRVAEGKELTEKFYRDASR
                     AAPTIDTYGQYREILERDDIDGVVLSLPDHQHAEIAIAAVRAGKDVYLQKPFTMTHAE
                     SVLLRDEVAKSGRVLQVGSQQRSWGETQQFRKGCEFVRSGRVGRLQRVEIGLPIDPTK
                     PDDPEQPVPANLDYDAWLGPTPKVYYTEQRVHPHSGYDRPGWLRNESYCLGMITGWGA
                     HHFDTAHWGMDMESSGPTKIEGTAEFPKNSIWNVHGAYEVALTYPGDIHMRVSDKLPN
                     GIKFIGDEGWIFVSREQQSAQTSSDPAIRATDLKPLDASDPKLLDPNGVTVKFLES"
BASE COUNT          241 a          442 c          397 g          263 t
ORIGIN      
        1 gctctcgagg aatttcaccg tgacgccgtt cgggtcgagc aacttcggat cgctcgcatc
       61 gagcggcttc agatcggtcg cgcgtatcgc cggatcgctc gaggtttgcg cgctctgctg
      121 ctcgcgcgag acgaagatcc aaccctcgtc gccgatgaat ttgatcccgt tcggcagctt
      181 gtccgagacc cgcatgtgga tgtcgccagg ataggtcagt gcgacttcgt aggcgccgtg
      241 cacgttccag atcgagttct tcggaaactc cgccgtgccc tcgattttcg tcggaccgct
      301 cgattccatg tccatgcccc aatgcgccgt gtcgaaatgg tgcgcgcccc agccggtgat
      361 catcccgaga cagtaggact cgttgcgcaa ccagccgggc cggtcgtaac cagaatgtgg
      421 atgcacgcgc tgctcggtgt agtaaacctt cggggtcggc ccgagccacg catcgtaatc
      481 gagattcgcc ggcaccggct gctcgggatc gtccggtttc gtcggatcga tcggcaggcc
      541 gatctcgacg cgctgcaagc gccccacgcg cccgctccgc acaaactcgc agcctttgcg
      601 aaactgctgc gtctcgcccc atgaacgctg ctggctgccc acctgtaaaa cgcgcccgct
      661 cttcgccact tcatcgcgca gcaacacgct ttccgcgtgc gtcatcgtaa acggcttttg
      721 caggtaaaca tccttgcccg cgcgcaccgc cgcgatcgcg atctccgcgt gctggtgatc
      781 cggcagactc agcaccacac catcgatgtc gtcgcgctcg aggatctcgc gatactggcc
      841 ataagtgtcg atcgtcggcg cggcgcgcga cgcgtcccga taaaacttct ccgtcaattc
      901 cttgccttcg gccacacgct tcgagtcgac gtcgcacaac gcgacgatgt cggccatgcc
      961 ggtcttgaac acgtcgggca tgtcgtgacc gcgcgcaatg cgcccgcaac cgatctggcc
     1021 gatccggaaa cggttgcttg gcgagttggc gcccagcagc cgcgatggga tgatggtcgg
     1081 caccgaaaac accgcgccgg cgatggcgac ggtgcggcag aaatcacgac gcgagagcga
     1141 accagtattt tttcggggtg aggttttcat ggggagtttt aaaagggatc ttgtagggcc
     1201 ggcgcttgcc gccgagccgc gactatcgaa aggcgaccgc cctaccgcat caatccatgc
     1261 gcgaattgct cacgaacgcg attcgcgtgc cgtccgggga ccacgatggg acgttgatcg
     1321 ttccctgtcc cccgaaaaca tac
//
LOCUS       DASPLT010000256         1229 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_167230, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000256 DASPLT010000000
VERSION     DASPLT010000256.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1229)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1229)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1229
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_167230"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1155
                     /locus_tag="VEA63_02740"
     CDS             <1..1155
                     /locus_tag="VEA63_02740"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HYD82931.1"
                     /translation="GGFRAGYEGSQDWDITLRFVEHLDPTRIHHIPRVLYHWRAIAGS
                     TALVVDQKSNYPFIAAKKALADHLTRAGVTAELVAVAGFHWRIKRALPATAPKVSIII
                     PTKNGENLLRLAIGSILAKTTYPKFEVIVVDNRSDDAGALAYFDELRRTGVRVLRYDE
                     PFNFSAINNFAVRAAEGSILAFLNNDLEVLTPDWLDEMVSHAIRPEIGCVGAMLYYPN
                     DTIQHAGAVLGLTGPAGKDGVAGHAFKCFPRGAEGQRNRLRLVQNYSAVTAACLVIRR
                     EIFDTVGGYNERDLAVAFNDIDFCLRVLKAGYRNLWTPFAEFYHHESASRGLEDTPEK
                     IARFQRETAYMRRMWAPLLDNDPAYNPNLSLQHEDFSLSFPPRTARLSGP"
BASE COUNT          235 a          471 c          301 g          222 t
ORIGIN      
        1 ggcggctttc gcgctggcta cgagggcagc caggattggg acatcacgct tcgcttcgtc
       61 gaacacctcg acccgacccg cattcaccac atcccccgcg tgctctacca ctggcgcgcc
      121 atcgccggat ccaccgccct cgtcgtcgac cagaagagca actacccgtt catcgccgcg
      181 aaaaaagctc tcgcggatca cctgacccgc gccggcgtca ccgccgaact cgtcgccgtc
      241 gccggctttc actggcgcat caaacgcgcc ctcccggcga ccgcaccgaa ggtttcgatc
      301 atcattccga cgaagaacgg cgagaatctc ctccggctcg cgatcggctc gatcctcgca
      361 aaaacaacgt atccaaaatt tgaagtcatc gtcgtcgaca accgctccga cgacgctggc
      421 gcgctcgcct atttcgacga gcttcgccgc accggcgtcc gcgttctccg ctacgacgaa
      481 cctttcaatt tttccgcaat caacaacttc gccgttcgcg ccgccgaagg ctccatcctc
      541 gcctttctca ataacgacct cgaagtcctc acgcccgact ggctcgacga aatggtctcc
      601 cacgccatcc gtcctgagat cggctgcgtc ggcgcgatgc tctactatcc gaacgacacc
      661 attcagcacg ccggcgccgt cctcggcctc accggccccg ccggcaagga cggcgttgcc
      721 ggccacgcgt tcaagtgctt cccgcgcggc gccgaaggcc agcgtaaccg cctccggctt
      781 gtccaaaact actccgccgt caccgccgcg tgcctcgtca tccgtcgcga aattttcgat
      841 accgtcggcg gctacaacga acgcgatctc gccgtcgcct tcaacgacat cgatttctgc
      901 ctccgcgtgc tcaaggccgg ctaccgcaat ctctggacac cgttcgccga gttctaccac
      961 cacgagtcgg cgagtcgcgg cctcgaagac acaccggaaa aaatcgcccg cttccaacgc
     1021 gagaccgcct acatgcgccg catgtgggcg ccgctccttg acaacgatcc cgcctacaac
     1081 ccgaacctca gtctccagca cgaggatttc tcgctctcct tcccgcccag gactgcacgc
     1141 ctctccggac cgtgaaatcc atccttcttg tggctctgag cgtgagctca gggactcgag
     1201 ggaggcacac tgataaatca actccagga
//
LOCUS       DASPLT010000257         1275 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_64370, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000257 DASPLT010000000
VERSION     DASPLT010000257.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1275)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1275)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1275
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_64370"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            584..>1275
                     /locus_tag="VEA63_02745"
     CDS             584..>1275
                     /locus_tag="VEA63_02745"
                     /inference="COORDINATES: protein motif:HMM:NF020252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HYD82932.1"
                     /translation="MSQLGHLLLDPNSEMGEITELLRRDTALTARIIRISNSAFYNSG
                     QPYGTLDEALARVGYMEVYRLTGLAAVSQMSDHELLLYGITGAQLRENSLLTALVMEA
                     LANRAQIDSRLAYTAGLLRSVGKIALDRLTRSGSYNGSYKGRDNGRLGDWETQFVGMN
                     NCEAAAVILKEWRFPLTTIAGIRDHYLGSPNASPLAQLLNLAAGAAERGGHGFPGETD
                     YWTGSLAACRDLS"
BASE COUNT          225 a          336 c          477 g          237 t
ORIGIN      
        1 gcgcgggggt ttgcgggcgg atttgcaacg cgggccgttg ctggatcacg cggggcaaag
       61 cgcgcggttc gcggcggcgt tgcgcgagtg ttggcgtgcg tgttgccgcg acgcgggtgc
      121 taagggcggg tagcacagag agcacggagc gcggacacag aggaagcagg aggcggcaag
      181 gtgtctggat gtttaggggc gggcgagagg agttgaccgg gcaaagtttg actaccgaag
      241 tcagagtttt gaaccgctaa tcagcgctga tttttcggag gacggcgacg caagcgaacg
      301 agtgcgtgct tcgcaatgcg ggcgtcggca agcgacgccc ctacggttgg atcggcgaag
      361 cgtggagctc gttcgggggt ctgcggggat tcaattagga ggtgcggttg agcgggcggg
      421 aggaggcgcc gcggaagcgt tgaaaaaggc gggcggcgcg cgcttttccg ctcatttcgc
      481 ggcgaatcgc gccgatagct gatgctgaac tatgatcgct acgccgattc cgcgcgagac
      541 gctgctgcac gtcgtcaagt ctttgccggc ggccccccgc atcatgtccc aactgggtca
      601 tttgctgttg gacccgaatt cggaaatggg ggagatcaca gagctgttgc ggcgcgatac
      661 ggcgctgacg gcgcggatca ttcggatcag caacagtgcg ttctacaatt cgggacagcc
      721 ctatggcacg ctcgacgaag cgttggcgcg cgtgggctac atggaagttt accgcctcac
      781 cggtctcgcg gcggtgtcgc agatgtcgga tcacgagctg ctgctctacg gcatcacagg
      841 ggcacagctg cgcgagaatt cgttgctgac ggcgctggtg atggaagcgc tcgcgaaccg
      901 cgcgcagatc gactcgcgcc ttgcttacac ggcgggcctg ttgcgatcgg tggggaaaat
      961 cgcgctcgac cggctgacgc ggagcggttc ctacaacggc agctacaaag gccgcgacaa
     1021 cggtcggctc ggcgattggg agacgcagtt cgtggggatg aacaattgcg aggcggccgc
     1081 ggtgattttg aaggagtggc gttttccgct gacgacgatt gcaggaattc gcgatcacta
     1141 cctcggcagc ccgaatgcgt cgccgttggc gcagttgttg aatctcgccg cgggtgcggc
     1201 ggagcgcgga ggccacggct ttccgggcga gacggattat tggaccggat cgctggcggc
     1261 gtgtcgcgat ctctc
//
LOCUS       DASPLT010000258         3929 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_249023, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000258 DASPLT010000000
VERSION     DASPLT010000258.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 3929)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3929)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3929
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_249023"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2128
                     /locus_tag="VEA63_02750"
     CDS             <1..2128
                     /locus_tag="VEA63_02750"
                     /inference="COORDINATES: protein motif:HMM:NF024371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3857 domain-containing protein"
                     /protein_id="HYD82933.1"
                     /translation="RASGAALNLDPTDPSSQAMSLRLAERMRDAAGMRAVCERILRAQ
                     PHHAEAQARLAQLGATDRAAKRRASPIVAPQPAEPSSENARSRRAWLTGQFGDNDSDA
                     AYLSDPAQTVASARQIADNPSGAALLSDVRIDRVRPNGLAASRVQQFFYVSTQAGAAR
                     FSTRSVQYSPTSESLTIVRARTHKRNGGFVEAVDEGDERVADASAAMYYDVRSRTVRF
                     ASVEPGDVIELDYRITPATDINEYGNYFASFQMFRTAVPERLKRYVVIASADRPLHFA
                     LQHMPEAQVSERGGERAYLWEARDLDALPNEPLGPAATEYAPYVHVSTIRDLQALGRW
                     YSQLLEPQLALSPELKQVAAAIAREHKKDIDRVNAVYRFALTSTHYVALEFGIHSYKP
                     YRVADVYARRFGDCKDKASLIIALLREMNIAAEFALVRTRPLGPIAENAASVALFNHA
                     VAYVPSLDLWLDGTADYYSLRELPVVDQNALALTVAADGSSRLRTIPGSRPEQNTIER
                     RVAARIQPDATIKFHGSSVTRGENAPALRRDFEVTERRRESVRRALADFFPTVKVDDV
                     RVDEATAERQVDMWFDGVLDSYAGQRVVPLRTSWLQRDYLQRLALLSERSQDLVLGVP
                     WTTQEEFWFDLPAGANVETPTALDVATPFGKATITYVHRANMLTIRSRVQFTTTRVSA
                     ADYPKFRAFCVELERAFRRDVKVTLP"
     gene            2125..>3929
                     /locus_tag="VEA63_02755"
     CDS             2125..>3929
                     /locus_tag="VEA63_02755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD82934.1"
                     /translation="MKLFRLLLALALATGISVAQTATDRASRALFESSDSATALRFAR
                     EALRRNPADVRAAFIAMEAAAVQENPAAVVDAALRVCASANPEDPRAEIATARLIAAA
                     KNTAAHRARLPRVKRLVAAHPELRLAQVAAAERGAPELDVLISSREAGLVTDWRVAGP
                     FGRHGMLDFDRQNPPEADGLRKKAYGKRKVEALQFGNGEFSLPRYFGTAGVFYAASDV
                     YVRSEGDWLLYVESPGTLEIFLDGARVVRRDDRAASKSHTIRTAVRVTKGDHRLLVKF
                     VASAAPFRVALLPPSGGRKRKNNIPLLRATPEDEYVIASLPLRRGDLVSAHRAASQFA
                     AKYPSALAHLLLAEVWQRSSEDAPEVESELRAAVAADPLAHAAAIKLAARQFGDDRIE
                     EGLRLLKRVLAHRPASERALSLAAYEMAHLGWNDEAAVFHDRIQALNPSCDVLLNAAR
                     FFASTANLRRAAELESRLATCAPGSLVYPRALAARGDHAGAAKAAAEVVKRFPLDRDA
                     RELAVREALLAGDIKGAAQHAEKLATMAPNSAEYQELSAMAARGLALPDTPKGIPVFA
                     FAPFRRDGLTVIRETAGQAYSGGPAVFVLNDGALVL"
BASE COUNT          655 a         1363 c         1227 g          684 t
ORIGIN      
        1 tcgggcgtcc ggcgcggcgc tgaacctgga ccccaccgac ccgtcatcgc aggcgatgtc
       61 gcttcgcctc gccgagcgta tgcgtgacgc cgccggcatg cgggcggtgt gcgagcgtat
      121 cctgcgagcg caaccgcacc atgccgaggc gcaagcccgg ctcgcccaac ttggagcaac
      181 ggatcgcgcc gccaaacgcc gagcgagtcc gattgtcgcg ccgcagccgg cggagccctc
      241 cagcgagaat gcacgctcgc gcagggcctg gctgaccgga cagttcggag acaacgactc
      301 cgacgctgcg tacctctccg atcctgctca gaccgtcgcc tcggcacgcc agatcgccga
      361 caacccatcc ggcgctgcgt tgctgtcgga cgtgcggatc gaccgggtac gcccaaacgg
      421 cctcgcggcg agtcgcgtgc agcagttctt ttacgtctcg acccaggcgg gagcggccag
      481 gttctcgacg aggtcggtgc agtactcgcc gacttcggaa tcgttgacga tcgtcagagc
      541 ccgcactcat aaacgaaacg gcggtttcgt cgaggctgtg gatgagggcg atgagcgcgt
      601 tgccgacgcc tcggcagcca tgtactacga cgtccgctcg cggaccgtcc ggttcgcttc
      661 cgtcgaaccg ggggacgtca tcgaactcga ctaccgcatc acgcccgcta cggacatcaa
      721 cgagtatggc aactacttcg cttcgtttca gatgttccgg accgcggtcc ccgaacgcct
      781 gaagcgatac gttgtgattg cttcggctga ccggccattg cacttcgcgc tgcaacacat
      841 gccggaagcc caggtttccg agcgcggcgg cgagcgcgcg tacctctggg aagctcgcga
      901 tctggacgcc ctgcccaacg aaccgcttgg gcctgccgca accgagtatg ccccgtacgt
      961 tcatgtctcg accatccgcg acctccaggc gctgggccgc tggtactcgc aactgctgga
     1021 gccacagctc gcgctgagcc cggaactgaa gcaagtcgcc gcggcgatcg cacgggaaca
     1081 caagaaggac atcgatcgcg tcaacgccgt gtaccggttc gcgctcacca gcacgcacta
     1141 cgtcgcgctc gaattcggaa tccacagcta caagccctat cgcgtggccg acgtgtacgc
     1201 ccgccgcttc ggcgactgca aagacaaggc gagcctgatc atcgccctgt tgcgtgagat
     1261 gaacatcgct gccgaattcg cgctggtccg cacgcgcccg ctgggtccta tcgcggagaa
     1321 tgcagcttcc gttgcgctct tcaatcacgc tgttgcctac gttccgagcc tcgatctctg
     1381 gctcgacggc acggccgact actacagcct tcgtgaactc ccggtcgtgg accagaacgc
     1441 cctggcgctg accgtcgccg ccgatgggtc ttcgcgattg aggacgatcc ctggcagccg
     1501 tcccgagcaa aacaccattg agcggcgagt tgccgcccgc atccagccgg acgcgaccat
     1561 caagttccac ggcagctcgg ttacgcgcgg tgaaaacgcg cccgcgctgc gccgcgattt
     1621 cgaagtaacg gagcgccggc gcgaatccgt gcgccgcgct cttgccgact tcttccctac
     1681 cgtgaaggtg gatgacgtgc gcgtggatga ggcaaccgcc gagcgacagg tggacatgtg
     1741 gttcgacggc gtcctcgaca gctacgcagg ccaacgggtt gtgccgcttc gcacgagttg
     1801 gctgcaacgc gactacctcc agcggctcgc actgctgagc gagcgcagcc aggacctcgt
     1861 gctcggcgtt ccgtggacga cacaggaaga gttctggttc gatctgcccg ccggcgccaa
     1921 cgtggagacc cccaccgcgc tcgatgtggc tacgccgttc ggcaaagcga cgatcacgta
     1981 cgttcatcgc gccaacatgc tgaccattcg ctcgcgcgta cagttcacga ccacgcgcgt
     2041 cagcgccgcc gattacccga agttcagagc tttctgcgtc gagctcgaac gagctttccg
     2101 tcgtgacgtg aaggtgacat tgccttgaag ttgtttcgcc tgctactcgc acttgctttg
     2161 gcgacgggca tttccgtcgc gcaaactgcg acggaccgcg cctccagggc gttgtttgag
     2221 agttccgatt ccgcaacggc gctccgcttt gcgcgtgaag ctcttcgccg caaccctgcc
     2281 gatgtccgcg cggccttcat cgccatggaa gccgccgccg tgcaggagaa cccggccgcc
     2341 gtcgtggacg cggcgcttcg ggtttgcgcc agcgcgaatc ccgaagaccc ccgggcggag
     2401 attgcaaccg cccggctcat cgccgcggca aagaacacgg ctgcgcatcg cgctcgcctc
     2461 ccacgagtga agcgcttggt agccgctcat cccgaactgc gccttgccca ggttgcggcc
     2521 gctgagcgcg gcgcgccgga actcgacgtg ctcatctcgt cgcgtgaagc cggactggtg
     2581 accgattggc gcgttgccgg tccgttcggc cgccacggca tgctcgactt cgatcgacag
     2641 aatccgccgg aggcggacgg tctccgcaag aaggcgtacg gcaagcgcaa ggtcgaagcg
     2701 cttcagttcg gcaacggtga gttcagtctg cctcggtatt tcggaaccgc cggtgtcttc
     2761 tacgcggcga gcgatgtgta cgttcgctcc gaaggggatt ggctgttgta cgtcgaatcg
     2821 cccggaacgc tggagatatt cctcgacggt gcacgggttg ttcgccggga cgaccgcgcc
     2881 gcctcgaaat cgcacaccat ccgcacggcg gtccgcgtga ccaagggcga ccaccgcctg
     2941 ctggtgaaat tcgtagcatc tgccgcgccg ttccgcgttg cgctgcttcc gccgagcgga
     3001 ggccgcaagc gcaagaacaa cattcccctg ctgcgcgcga ctcccgaaga cgagtatgtc
     3061 atcgcgtccc tgccgctccg gcgcggcgac ctcgtctccg ctcatcgcgc cgcctcgcag
     3121 ttcgcggcca aatacccgtc ggcgttggct cacctcctac tggccgaggt gtggcagcgc
     3181 tcgtcggagg acgcgccgga ggtggaatcg gagttgcggg ccgcagttgc cgccgatccg
     3241 ctcgcccacg ctgccgccat caaactcgct gcgcgccagt tcggcgacga ccgcatcgaa
     3301 gaggggctgc gtcttctcaa gagagtgctc gcgcatcggc ctgcgtccga acgcgcgctc
     3361 tcgctggcgg cctacgagat ggcgcacctg ggatggaacg acgaggctgc cgtctttcac
     3421 gaccgaatac aagccctgaa cccgtcttgc gacgtgctgc tcaacgcggc acgttttttc
     3481 gcttctacgg ccaatctccg gcgtgccgcc gagcttgagt cacgcctggc cacgtgcgcg
     3541 ccgggatcgc tcgtttaccc acgggcgctc gctgcccgcg gcgaccatgc tggagcggcg
     3601 aaagcggcgg ccgaggtggt gaagcggttc cctctggacc gcgacgcgcg cgaactcgcg
     3661 gtccgcgaag ccttgctcgc cggcgacatc aagggcgcag cgcagcatgc cgaaaagctg
     3721 gcgacgatgg ctcccaactc cgccgagtac caggaactct ctgcgatggc cgccagggga
     3781 ttggcactgc ccgatacgcc caaaggcatc ccggttttcg cctttgctcc atttcgccgg
     3841 gacggcctca cggtcattcg ggaaaccgcc ggccaggcgt actccggcgg ccccgccgtc
     3901 ttcgttctga acgacggtgc cctcgtcct
//
LOCUS       DASPLT010000259         3131 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_290654, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000259 DASPLT010000000
VERSION     DASPLT010000259.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 3131)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3131)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3131
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_290654"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..324)
                     /locus_tag="VEA63_02760"
     CDS             complement(<1..324)
                     /locus_tag="VEA63_02760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD82935.1"
                     /translation="MANVRAENAGRARRAWEHGGALTMNAGVKVPRVALIGISGYGRI
                     HLQLARECRDRGEIAIAAATVINPGEEAANVAELEAHGTKIYADYAEMLRAEAGRIDL
                     CLIPTG"
     gene            623..>3131
                     /locus_tag="VEA63_02765"
     CDS             623..>3131
                     /locus_tag="VEA63_02765"
                     /inference="COORDINATES: protein motif:HMM:NF028442.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HYD82936.1"
                     /translation="MSRLLALLLATLAVAASAEPITPVWPEVRRESKPWTRWWWLGNI
                     ADEKTITTAMESYVAAGLGGLELTPIYGVRGYEDRFVPFLSPRWTQLFEYTLAEAHRF
                     DLGIDLANGTGWPFGGPWLAEADAAHTVTHQTFHVAAGQSLAEPVTFTPRGVVRFAGA
                     TRVPLAEIQHPVTANANLQELAIDQIRFEQPARLVTLMAFSANTTPVDLTPHVRPDGR
                     LDWTAPADAGEWTLHALFEFPHGKMVERAAPGGEGLALDHFSSTALKNYLEKFSATLP
                     VCHLLDDKHPCVRAFFNDSYEVDDSEGEADWTPRFLAEFEKRRGYDLRQHLPALFSGN
                     DTRVLSDYRETISDLLLDEFTTPWREWAHARGSMIRNQPHNAPANILDLYAASDIPEQ
                     EGNGLLAMKLASSAAHLTGKPLASAEIATWLNDHFLTTHAELKANADTFLLAGLNHLF
                     YHGTALSPSDDPWPGFQFYASVELNPANPFWSDFAALNIYLTRVQSFLQSGRPDEDVL
                     LYYNIHDTWAQRGDGTLPHFGHGGENPVGVSAATLAAELQAAGVGFDYISDRLLQDVT
                     VDGTAGQKGAASLRSANVSYRTILLPEITYLPEATLAKLAQLAEQGATILVHRDLPKN
                     AAGFRANEIAFAQTLEKIRRRARTDTDGVVTANIGEGRFVIGENLPALLARHTIPREP
                     LNQFGLDFIRRRTDDGFVYFLVNRSNAPIDGWVPLNHATAHTVLFDPITGAIGAAATR
                     ESPHSEIYLQLEPRASIIVRTYTSDVSTPPWSYSQPAAPATALVGEWAVAFERGGPTL
                     PAPTRISTLTSWTDFTAPDRDALNAFSGTATYALTFSR"
BASE COUNT          557 a         1268 c          766 g          540 t
ORIGIN      
        1 ccccgtcgga atcaaacaca ggtcgatccg ccccgcctcc gcgcgcaaca tctccgcgta
       61 gtccgcgtaa atcttcgtcc catgcgcctc gagctccgcg acgttcgccg cctcctcgcc
      121 gggattgatc accgtcgccg ccgcgatcgc gatctcgccg cgatcccgac actcccgcgc
      181 cagttgcaaa tgaatgcgcc cgtaaccgga aatcccgatg agggcgaccc gtggaacttt
      241 tacgccagcg ttcatcgtca acgcgccgcc gtgctcccac gcccgccgcg cccgaccggc
      301 gttttcagcg cggacgtttg ccatggcagc agcgcgccag cgcgcaatcg acacgaaaca
      361 aggaaaggaa gaagtcccat tttcacgggc gggtggggaa aattttctgc aagcgagcgc
      421 cgattcggtc cggcgttttc gaaggcggcg caggattgtt tccaccgaag ggcgaagcca
      481 tgcgtcgacc gtcttgacag tccgagtcgc ccgccattgt cgccgccgcc gccgtgcttc
      541 gacctgtcgg cagccaccct ccttgcaacg tccccaacga acgcgtggcg ctcgcgccac
      601 ccttcactag ctttccttcc ccatgtctcg ccttcttgct ctcctcctcg ccacccttgc
      661 cgtcgctgcg tccgccgaac ccatcacacc cgtctggccc gaggtgcgcc gcgagtccaa
      721 gccgtggacg cgctggtggt ggctcggtaa catcgccgac gagaaaacca tcaccaccgc
      781 aatggagagc tacgtcgccg cgggtctcgg cggactggag ctcacgccca tctacggcgt
      841 gcgcggctac gaagatcgct tcgttccatt cctctcgccg cgctggactc agctttttga
      901 atacacgctc gccgaagctc accggttcga tctcggcatc gatctcgcca acggcaccgg
      961 ctggcccttc ggtggaccct ggctcgccga agcagacgcc gcccacacgg tcacgcacca
     1021 aaccttccac gtcgccgccg gccagtcgct cgccgaaccc gtgaccttca ctccgcgcgg
     1081 cgtcgttcgc ttcgccggcg cgacccgcgt gccgctcgcc gaaatccaac accccgtcac
     1141 cgccaacgcc aacctccagg aactggcgat tgaccaaatc cgcttcgaac aaccagcgag
     1201 gctcgtgacg ctcatggcgt tttccgccaa cacgactccg gtcgatctca cgccgcacgt
     1261 tcgccccgac ggccgtctcg actggaccgc acccgccgac gccggcgaat ggacgctcca
     1321 cgcgctcttc gaattcccgc acggcaagat ggtcgagcgc gccgcccccg gcggcgaagg
     1381 cctcgccctc gatcacttct cctccaccgc gctcaaaaac tacctcgaaa agttctccgc
     1441 caccctgccc gtttgtcatc tattagatga caaacacccc tgcgtccgcg ccttcttcaa
     1501 cgactcctat gaggtcgacg actccgaggg cgaagcggat tggacgccgc gcttcctcgc
     1561 cgagttcgag aaacgccgcg gctacgatct gcgccagcac ctgcccgcgc tcttctccgg
     1621 caacgacacc cgcgtgctca gcgattatcg cgagaccatc tccgatctgc tcctcgacga
     1681 gttcacgact ccctggcgcg aatgggcgca cgcccgcggc tcgatgattc gcaaccagcc
     1741 acacaacgcc cccgcgaaca ttctcgatct ctacgccgcc agcgacatcc ccgagcagga
     1801 aggcaacggc ctgctcgcga tgaaactcgc ctcgtccgcc gcccacctca ccggcaaacc
     1861 gctcgcttcc gccgaaatcg ccacgtggct caacgatcat ttcctcacca cgcacgccga
     1921 actcaaagcc aacgccgaca ccttcctcct cgccggcctg aatcaccttt tctaccacgg
     1981 caccgccctc tcgccctccg acgatccgtg gccgggtttt caattctacg cctccgtcga
     2041 gctcaacccc gcgaatcctt tctggtccga cttcgccgcg ctcaacatct acctcacccg
     2101 cgtccaatcc ttcctccaat ccggccgccc cgacgaagac gtcctcctgt attataatat
     2161 ccacgacacg tgggcgcagc gcggcgacgg cacgctcccg cacttcggcc acggcggcga
     2221 aaatcccgtc ggcgtttccg ccgccaccct cgccgccgaa cttcaagccg ccggcgtcgg
     2281 cttcgactac atctccgacc gcctcctgca agacgtcacc gtcgacggaa ccgcgggcca
     2341 aaaaggagcc gcgtctcttc gctccgccaa cgtttcctac cgcacgatcc tgctcccgga
     2401 gatcacctac cttcccgaag caacgctcgc aaaactcgcc cagctcgccg agcagggcgc
     2461 gaccatcctg gttcatcgcg acctcccgaa aaacgccgcc ggcttccgcg caaacgaaat
     2521 cgctttcgct caaaccctcg aaaaaatccg ccgccgcgcc cgcaccgaca ccgatggcgt
     2581 cgtcaccgcc aacatcggcg aaggccgttt cgtcatcggc gaaaatcttc ccgcgctact
     2641 cgctcgtcac accattcccc gcgagccgct gaaccagttc ggcctcgatt tcatccgccg
     2701 ccgcaccgac gacggtttcg tctacttcct cgtcaatcgc agcaacgccc cgatcgatgg
     2761 ctgggtgcct ctcaaccacg cgacagcgca caccgttctc ttcgacccaa taaccggcgc
     2821 tatcggtgcc gccgccaccc gcgaatctcc ccactccgaa atctacctcc aactcgaacc
     2881 ccgcgcatcg atcatcgtcc gcacctacac gtccgatgtc tcaactccgc cgtggtccta
     2941 ctctcaaccc gccgcgcccg ccaccgcact cgtcggcgaa tgggcggtcg ccttcgaacg
     3001 cggcggcccg acgcttcccg cgcccacgag aatctccacg ctcacgtcct ggactgattt
     3061 caccgcgccc gatcgcgacg ccctcaacgc gttttccggc accgccacct acgcgctcac
     3121 gttctctcgt c
//
LOCUS       DASPLT010000260         1453 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_25702, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000260 DASPLT010000000
VERSION     DASPLT010000260.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1453)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1453)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1453
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_25702"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1324
                     /locus_tag="VEA63_02770"
     CDS             <1..1324
                     /locus_tag="VEA63_02770"
                     /inference="COORDINATES: protein motif:HMM:NF044972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HYD82937.1"
                     /translation="IAATSALIAQSAPPPAAESAPAAPTSSAGATEAQAATNTKAAAA
                     VASGQAESPRTPGFLEHLVDGVLEALNIRTSTNTVTHYAISAILLVIALLARRIVTHV
                     LFPILRKVASRTRTTFDDRLFAATESPISTFIMLVGIFGALRVLKLSPSADIAISYGS
                     RVAFSLAVFWLLWRGLQAVLDHGAEIANARQLGIAAFMPWIKKSLMTVFVVLGVLLTI
                     QGLGYDVKAILTGLGIGGLAFALAAQDTLANIFGAIVVAVDQPFRIGETVKIQGNVGG
                     VEDIGVRSTRIRLIDGSLMVIPNKIVAAETITNLSRFIKRRFEQVIGLTYDTRPEDME
                     AIVEEIRGIIKSQPEVGPNPLVFFRDLNASSLDIWIVYEIPDPDFARAMNCKQRINFL
                     IMRAVEARGLSFAFPTQTLHLESEAIARIADRSNEPPPNAGRPPRLEQ"
BASE COUNT          266 a          548 c          363 g          276 t
ORIGIN      
        1 gattgcggcg acctccgcgc ttatcgctca atccgccccg ccgcccgccg cggaatcagc
       61 acccgccgcc ccgacgagca gcgccggcgc caccgaagcg caagccgcca ccaacaccaa
      121 ggctgcggcc gccgtcgcct ccggtcaggc cgaatctccc cgcactcccg gcttcctcga
      181 acacctcgtg gacggcgtgc tcgaagcgct gaacatccgc accagcacca acaccgtcac
      241 gcattatgcg atcagcgcca tcctcctggt catcgcattg ctcgcccgcc gcatcgtcac
      301 tcacgtgctt ttcccgattc tccgcaaagt ggcttcgcgc acgcgcacca cgttcgacga
      361 ccggctcttc gccgccaccg aatcgcccat ttccaccttt atcatgctcg tcggcatctt
      421 cggcgcgctg cgagtcctca agctctctcc ttccgcggat atcgccatct cctacggctc
      481 ccgcgtcgcg ttctccctcg cggtgttctg gctcctttgg cgcgggctgc aagccgtcct
      541 cgaccacggc gccgagatcg ccaacgcgcg tcagctcggc atcgcggcct tcatgccatg
      601 gatcaaaaaa tcgctgatga ccgtgttcgt cgtcctcggc gtgttgctca cgatccaggg
      661 cctcggttat gacgtgaaag ccattctcac cggtctcggc attggcggcc tcgcctttgc
      721 cctcgctgcg caagacacgc tcgcgaacat cttcggcgcc atcgtcgtcg ccgttgatca
      781 acccttccgc atcggcgaaa ccgtgaaaat ccagggcaac gttggcggcg tcgaagacat
      841 cggcgtgcgc tccactcgca tcaggctgat cgacggttct ctcatggtga tccccaacaa
      901 gatcgtcgct gctgaaacga tcaccaatct ctcccgcttc atcaaacgcc gcttcgagca
      961 agtgatcggt ctcacctacg acacccgccc cgaggacatg gaggccatcg tcgaagagat
     1021 tcgcggcatc atcaaatccc agcccgaggt cggaccgaat cccttggtgt tcttccggga
     1081 tctcaacgcc tcctcgctcg atatttggat cgtctacgaa atccccgatc ccgatttcgc
     1141 ccgtgcgatg aactgcaaac agcgcatcaa cttcctcatc atgcgcgccg tcgaagcccg
     1201 cggcctgtcg ttcgcctttc cgacccagac ccttcacctc gaaagcgaag cgatcgccag
     1261 aattgccgac cgcagcaacg agcctccgcc caatgccggc cgccctccgc gactcgagca
     1321 gtagaagtaa aacctgcttg ctggcgattg ctcgcctcaa tccagcgtct gtcgataccg
     1381 cttcacgccg acggtcattg cgaccacgag aaacaacagc agcggccaca ggtgcggcgc
     1441 gatctcggca aac
//
LOCUS       DASPLT010000261         1052 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_3531, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000261 DASPLT010000000
VERSION     DASPLT010000261.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1052)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1052)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1052
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3531"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..227)
                     /locus_tag="VEA63_02775"
     CDS             complement(<1..227)
                     /locus_tag="VEA63_02775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD82938.1"
                     /translation="MERGRHLVHEVAMCIDCHSPRGHDGQFLAGKHLAGSPLEIGPLV
                     PMPAWAPTAPAIKGLPGYTDEQAAHFLMTGKR"
     gene            complement(383..>1052)
                     /locus_tag="VEA63_02780"
     CDS             complement(383..>1052)
                     /locus_tag="VEA63_02780"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HYD82939.1"
                     /translation="RTAEDLMDGEPVSFDVHRRASDPRLQHLAMALLEEAKTARSSER
                     LFGEALATALAGHLIRDYASRARELDSIANASAQGGLPRVKLLHAIDYIHDQLHTDLT
                     IAGIARAVFLSPYHFTRLFKQSTGRSPYQYVIEARLKKARELLVSGDHTINDVALQTG
                     FSDQSHLTRHFRNAYGRTPKAALAEHAVGRVPAAHRIADRRFLPKHRTMIQEERSTLG
                     YGSR"
BASE COUNT          193 a          298 c          359 g          202 t
ORIGIN      
        1 cgcttcccgg tcatcaaaaa atgagccgct tgttcgtctg tgtagcctgg gagtcctttg
       61 atcgccggag cggtgggcgc ccacgcgggc atcggcacga ggggtccgat ttcaagtgga
      121 gaaccggcaa ggtgtttgcc agccaggaac tggccgtcgt ggccgcgcgg cgaatggcag
      181 tcgatgcaca tcgccacctc gtggaccagg tgacggccgc gttccacgcg ggcggcggtt
      241 tcggaacggc gggccgaagc cggcgtcgta aatccgaaca ggacgctcgc gccaacgaga
      301 agggacgtgc ggatgatgct acggctaaca ggcgagcctg ggagcgcgac cagcgtcgcc
      361 gcggtgggaa gatttttcgg tttcatctcg aaccgtagcc caaggtggag cgctcctctt
      421 gtatcatcgt gcggtgtttt gggagaaacc tgcggtcggc gatccgatga gcggccggca
      481 ctcgaccgac agcatgctcc gccagcgcag ctttgggcgt cctcccgtag gcgtttcgaa
      541 aatgacgcgt caaatgactt tggtcggaaa acccggtctg gagcgccacg tcgttgatgg
      601 tgtgatcgcc cgagaccagc agctcgcggg cttttttcag ccgtgcttcg atcacgtatt
      661 ggtagggaga gcggccggtg gattgcttga agagccgcgt gaaatgatag gggctcaaga
      721 agaccgcccg cgcgattccg gcaatcgtca gatcggtatg cagttgatcg tggatatagt
      781 cgatcgcgtg aaggagtttg actcgcggaa gtccgccttg cgccgaggcg tttgcgatcg
      841 aatcgagctc tctcgcccgg gaggcgtaat cgcggatgag atgcccggcg agcgccgtgg
      901 ccaacgcctc gccaaagaga cgttcgctcg agcgcgccgt tttcgcttct tcgagcaatg
      961 ccatcgcgag gtgttgaagc cgcggatcgg aagcgcggcg atgcacatcg aacgacaccg
     1021 gttcgccgtc catcagatcc tcggcggttc gg
//
LOCUS       DASPLT010000262         1308 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_290673, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000262 DASPLT010000000
VERSION     DASPLT010000262.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1308)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1308)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1308
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_290673"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1308)
                     /locus_tag="VEA63_02785"
     CDS             complement(<1..>1308)
                     /locus_tag="VEA63_02785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD82940.1"
                     /translation="VYQLTPEPRGGWLDWVGRQRINLHAERRELENVNYRYGDFVASS
                     HPWITPSNRSASTTTYFQYYLGDSQGQNIDYAPTSRSNLAGDYTFNWFNAQLNQWVAE
                     PATLDQVPISNTRTRTRYIESANVTYQGSFLADRVVATAGYRRDEQGSNTSDAWTIDP
                     DTGLYDHDSEALGIFPSGDTVEKGDTYTLGLVLKPRPWLRLFYNWADSFSPEAVRYDI
                     NGIQMPNPTSEGQDFGVGVSLFDGKLDLKINHYEVKEIGARLSQLGTVGSRTHVLEGG
                     RDQSPQSFLGWAMGVVRDRYQRQGITATEDQIYDQAATEFVKLDPGFLRQTDLTGAVG
                     VGGDRESKGWELEAVYNPTRNWRIKFNAAQQKSVDSNIGGELDDYFARRLPVWTTAKD
                     DAGNLWWNANNNYAVNQWLGSIRAPYLFEVANDGKSRAQVREW"
BASE COUNT          231 a          368 c          436 g          273 t
ORIGIN      
        1 gccattcgcg aacctgcgcg cgggacttgc cgtcgttggc gacctcgaag agataaggcg
       61 cgcggatgga gccgagccat tgattgacgg cgtagttgtt gttggcgttc caccagaggt
      121 tgccggcgtc gtccttggcg gtggtccata ccgggagacg gcgcgcgaag taatcgtcga
      181 gttcgccgcc gatgttggag tcgaccgatt tctgctgggc cgcgttgaac ttgatgcgcc
      241 agttgcgcgt cgggttgtaa accgcttcga gttcccagcc tttcgattca cggtcgccgc
      301 cgacgccgac cgcgccagtg aggtcggttt gacgaaggaa gccagggtcg agcttgacga
      361 attccgtggc cgcctggtcg tagatctggt cctcggtcgc ggtgatcccc tgtcgttggt
      421 agcgatcgcg gaccacgccc atcgcccaac caaggaaaga ttgcggactt tggtcgcggc
      481 cgccttccag gacgtgcgtg cgggacccaa ccgtgccaag ctggctgagg cgggcgccga
      541 tttccttcac ttcgtaatgg ttgatcttga gatcgagttt gccgtcgaac agcgagacac
      601 cgacgccgaa atcctggcct tcgctcgtcg gattgggcat ctgaattcca ttgatgtcgt
      661 agcggaccgc ttccgggctg aagctgtcgg cccagttgta gaaaagccgc agccacggac
      721 gtggcttgag caccagacca agcgtgtagg tgtcgccctt ctccaccgta tcaccggaag
      781 gaaaaatgcc cagcgcttcg gagtcgtgat cgtagagacc tgtgtccgga tcgatggtcc
      841 aggcatcgga ggtgttgctg ccctgctcgt cgcgacgata gccggcggtc gcgacaacac
      901 gatcggcgag gaaggaaccc tggtaggtga cgttggccga ttcgatataa cgggtccggg
      961 tgcgcgtgtt gctgatcggc acctgatcga gcgtggccgg ctcggcgacc cactgattga
     1021 gttgggcgtt gaaccaattg aacgtgtagt cgcccgcgag attgctccgg gaagtcgggg
     1081 cgtaatcgat gttctgcccc tggctgtcgc cgagatagta ttggaagtaa gtcgtggtgg
     1141 aggccgagcg gttggacggc gtaatccacg ggtgcgacga agccacgaaa tcgccgtagc
     1201 ggtagttcac gttttccaac tcgcgacgtt cggcgtgcag attgatccgt tgccggccga
     1261 cccagtcgag ccatccgccc cgcggttcag gggtgagttg atacacca
//
LOCUS       DASPLT010000263         1060 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_105016, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000263 DASPLT010000000
VERSION     DASPLT010000263.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1060)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1060)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1060
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_105016"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..271
                     /locus_tag="VEA63_02790"
     CDS             <1..271
                     /locus_tag="VEA63_02790"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HYD82941.1"
                     /translation="MVSNLKEATASAREADNAAIKAHEQRSHVLPLAEGATAPPKLPS
                     LQQPGEGIGLSIVKRLCELLDASLELASSVTSGTVFRVVLPRYYE"
     gene            392..583
                     /locus_tag="VEA63_02795"
     CDS             392..583
                     /locus_tag="VEA63_02795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD82942.1"
                     /translation="MDTRSSLPAYVLDQDLQIVRATLATISEDVNRLSPPAGRAVHDA
                     LEKIDQAHAEFVRSANGDD"
     gene            780..>1060
                     /locus_tag="VEA63_02800"
     CDS             780..>1060
                     /locus_tag="VEA63_02800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009510816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HYD82943.1"
                     /translation="MTTRDSTLTLAVFLDLENIAIGAREAHFPAFDIGKALERLLLKG
                     NIVVKKAYCDFERFADFKRPLHEAAFELIEIPHVRQSGKNSADIRMVVDA"
BASE COUNT          199 a          381 c          285 g          195 t
ORIGIN      
        1 gatggtgagc aatttgaaag aggcgaccgc cagcgcgcgc gaggccgaca acgccgccat
       61 caaggcgcac gagcaacgtt cgcatgttct tccgctcgcc gaaggcgcca cggcgccgcc
      121 caaacttcct tcccttcaac agccgggcga aggcatcggg ctctcgatcg tgaaacgcct
      181 ctgcgaactg ctcgatgcca gcctcgagct cgcgagctcc gtcacgtccg gcaccgtttt
      241 ccgcgtcgtg ctgccgcgtt actacgaata gacggcgtcg agacgcgcca tcgacctcgg
      301 cgcgcttcgt catcgattgt gccgtggcgt tcaaaatttc gccgtcgaaa tttttggaac
      361 tcgctctggt tgcaacgcgt ccgaaacgcg catggacacc cgctcttcgt tgccagctta
      421 cgtgctcgat caggacctcc aaatcgtccg tgcgacgctc gccaccattt ccgaagatgt
      481 gaaccgtctc tcgccgcccg ccggtcgcgc cgtccacgac gcgctcgaaa aaatcgacca
      541 ggcgcacgcc gagttcgttc ggtcagcgaa cggcgacgac tgaggcatcc gcgaaccgtt
      601 cgcagccgcc gccaaagcgt gtcaccccgg cccgaggatc accgcgcagt cgccgaggtc
      661 cgggcggctt cgggaattcg aactcccgcc actattagcg ttcattagcg tggattagcg
      721 gttaactctc ccgcaaacgg cgccccttcc cgcgcgccgc gctccaatcc gctccgccca
      781 tgaccacccg cgattccacc ctcacgctcg ccgttttcct cgacctcgag aacatcgcca
      841 tcggcgcgcg cgaagcacat ttcccggcgt tcgatatcgg caaggcgctc gaacgtctcc
      901 tgctcaaggg caacatcgtc gtcaaaaaag cttactgcga cttcgaacgc ttcgccgatt
      961 tcaaacgccc gctccacgag gcggcattcg agcttatcga gattccgcac gtccgtcagt
     1021 cgggtaaaaa ctcggccgac atccgcatgg tcgtcgacgc
//
LOCUS       DASPLT010000264         1169 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_125792, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000264 DASPLT010000000
VERSION     DASPLT010000264.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1169)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1169)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1169
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_125792"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1169
                     /locus_tag="VEA63_02805"
     CDS             <1..>1169
                     /locus_tag="VEA63_02805"
                     /inference="COORDINATES: protein motif:HMM:NF013591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HYD82944.1"
                     /translation="VKFEEDLVAGLEPKYAAEMAVLRALKSKETGAANTTLEPWDVSY
                     YLNQLKKQRYAVDAEGLRVYFAYQQTLDGMFALYQKLFGLTFTRVEPPQVWAPGVQLF
                     VVTDAVTTVPIGSFYLDMFPREGKFNHFACFPQMSGRVLGDGRYELPVATLVCNFPPA
                     SADKPSLLSHNDVETLFHEFGHVMHMLLGRARFQGQTSGAVPRDFVEAPSQMLENWVW
                     DKAVLDTFAADYRDRSKKIPASTIAALTRAREATDGYFNRRQLSLGLVDLTLHTRSAE
                     EAKHLDVVGETNAVFGRVTIPPAPETAFVAYFGHLAGYDAGYYGYLWSKVMAIDMASE
                     FKKAPDGFLDVSVGRRLREEVYAVGDTRDVAESVEKFLGRPRSMKPFLEYVGIKE"
BASE COUNT          209 a          337 c          392 g          231 t
ORIGIN      
        1 ggtgaagttc gaagaggacc tcgtcgccgg gctcgagccc aagtatgcgg ccgagatggc
       61 ggtgttgcgg gcgttgaaga gcaaggaaac cggtgcggca aacacgaccc tcgagccgtg
      121 ggacgtcagc tattatctga accagttgaa gaagcagcgc tacgccgtcg atgccgaggg
      181 gctgcgtgtt tattttgcgt atcagcaaac cttggacggc atgttcgcac tctatcagaa
      241 gctgttcggg ctgacattca cgcgtgtgga gccgccgcaa gtgtgggccc cgggcgtgca
      301 gttgtttgtg gtcacggatg ccgttacgac cgtgccaatc ggcagcttct acctcgacat
      361 gtttccgcgc gaggggaagt tcaaccactt cgcctgcttc ccgcagatgt cgggccgcgt
      421 gctcggcgat ggtcgctacg agctgccggt cgccacgctc gtgtgcaatt ttccgcctgc
      481 gtccgcggac aagccgtcgc tgctgtcgca caacgacgtg gaaacgctgt tccacgaatt
      541 cggccacgtg atgcacatgc tgctcggccg cgcgcgcttc caaggccaga cctccggcgc
      601 cgtgccgcgc gatttcgtcg aggcgccatc gcagatgctg gagaactggg tgtgggacaa
      661 ggcggtgctc gacacgtttg cggcggatta tcgggatcgt tcgaaaaaaa taccggcgtc
      721 gacgatcgct gcgctcacgc gtgcgcgcga ggcgaccgac ggctacttca accgccgcca
      781 gctttcgctt gggctggtcg atctgacgtt gcatacgcgc tcggcggaag aggcgaagca
      841 tctcgacgtc gtcggcgaaa cgaatgcggt gttcggtcgc gtcacgatcc cgcctgcgcc
      901 ggagacggcg ttcgtcgcgt atttcggcca cctcgcgggc tacgacgccg gttactacgg
      961 ctatctttgg tcgaaggtga tggcgatcga catggcgagc gagttcaaga aagcgccgga
     1021 cggttttctc gatgtcagcg ttggacgccg gctgcgcgag gaagtgtatg cggtgggcga
     1081 cacgcgcgac gtggcggagt cggtggaaaa attcctcggc cgcccgcgct cgatgaaacc
     1141 gtttctagaa tacgtcggga tcaaggagt
//
LOCUS       DASPLT010000265         1122 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_290698, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000265 DASPLT010000000
VERSION     DASPLT010000265.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1122)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1122)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1122
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_290698"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..417)
                     /locus_tag="VEA63_02810"
     CDS             complement(<1..417)
                     /locus_tag="VEA63_02810"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HYD82945.1"
                     /translation="MTPTLPCLPRRLPTFRKSAGFSLAVSILLFAGASGVYAQDSAAS
                     AAIDDKVIQLDTFEVTGLRSSAIKALEIKRNSPQIMESIVAEDIGKFPDNNAVESLQR
                     LPGVQVTDYGAGNIASVTIRGLPDVTTTLHGRNIFTA"
     gene            complement(681..>1122)
                     /locus_tag="VEA63_02815"
     CDS             complement(681..>1122)
                     /locus_tag="VEA63_02815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="serine/threonine protein kinase"
                     /protein_id="HYD82946.1"
                     /translation="ISLLQPASARLLEAMKPLAEATVQALAEADAGGDTFTVAWRSLS
                     MALLEYRRGNYADAMRWSARCLGNAETNAPRSATARLITAMAHFQLGQGAEAQAELER
                     VREVIAVKFEGVGDRGSPIRGFWFDWMFARVLLREASGLVGAGL"
BASE COUNT          228 a          412 c          291 g          191 t
ORIGIN      
        1 cgccgtaaaa atattccggc catgcaatgt cgtggtgacg tccggcaggc cgcgaatcgt
       61 caccgatgcg atgttacccg cgccgtagtc ggtgacctgc acgccaggca ggcgttgaag
      121 cgactcgacc gcgttgttgt ccgggaactt gccgatatct tccgcgacaa tcgactccat
      181 gatctgcgga gaattgcgct tgatctcgag cgccttgatc gcgctggagc gcagaccggt
      241 gacttcaaac gtatccagtt ggatcacttt gtcgtcgatc gccgccgagg ccgcagagtc
      301 ttgggcgtaa acaccactgg cgccggcgaa aagcaggatc gaaacagcga gcgagaaacc
      361 agcggatttc cggaacgtcg gaagccgccg cggaaggcag ggcagggtcg gggtcataag
      421 cttggggtta tggtcggatt gcgtgttccc accggattca cgcgacaaca acgcgtcgac
      481 gcggattcca atcggaagtg ttacttcacc tctccatgcg ctgttcgggc agcaaaaccc
      541 gacaggccgc aaaaaaaaat ttcggcaagg ccggcgcctg cttgcaggcg atcacacgcg
      601 ccgaacacag gcgacgcacc tcatcccctg caggcagcct cctaccctca acccttcaac
      661 ccttcattcc gcccaccctc tcacaacccc gcgccaacca acccactcgc ctctcgcaac
      721 aacacccgcg caaacatcca gtcgaaccaa aacccgcgga tcggacttcc ccgatcaccc
      781 actccttcaa atttcaccgc gatcacctcg cgcacccgct ccaactccgc ctgcgcctcc
      841 gctccctgcc ccaactggaa atgtgccatc gccgtgatca gccgcgccgt cgcggaccgc
      901 ggcgcatttg tctccgcatt gccgagacac cgcgcgctcc accgcatcgc gtccgcgtag
      961 ttgccgcgtc gatactccag caacgccatc gacagcgacc tccacgccac cgtgaacgtg
     1021 tcgcccccgg cgtccgcctc ggccagtgcc tgcacggtcg cttccgccag cggcttcatc
     1081 gcctccaaca accgcgcgct cgccggctgc agcaaactga tc
//
LOCUS       DASPLT010000266         1033 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_249073, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000266 DASPLT010000000
VERSION     DASPLT010000266.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1033)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1033)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1033
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_249073"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            176..937
                     /locus_tag="VEA63_02820"
     CDS             176..937
                     /locus_tag="VEA63_02820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HYD82947.1"
                     /translation="MSSSAARPASSVPAHVAIIMDGNGRWAKQRGLPRIEGHRRGVET
                     VRTATFAARDLGVKMLTLYAFSVENWKRPQDEVGALMGLLEYYLRKELETFVRDRVRL
                     RTIGRTSDLPAGVQKLLRTTIEETKHFADYTLVLALNYGARTEIVDAARAYAAAVAAG
                     TEKLNDSSWDTFSRYLYTADLPDPDLIIRTSGEKRISNFLLMQGAYAEYVFTPVLWPD
                     FTKADLAAAIAEYSRRERRFGQTSEQVKPAVAATK"
BASE COUNT          176 a          412 c          250 g          195 t
ORIGIN      
        1 cgatctaggc ctttgtcatc tattgtatga caaaatgcca cgacgcccgc tctccggccc
       61 gctcccttct tttccttctc tcacggtcgt tcccgtcgat tttcgccgcg cgctcacttc
      121 ccgcgaagcc ggcttgtcaa agccagcctc gggttcctaa ctcggcgcgc tgagcatgtc
      181 atcttccgcc gcccgtcccg cttcttccgt cccggcccac gtcgccatca tcatggacgg
      241 caacggtcgc tgggccaaac aacgcggcct gccccgcatc gagggccacc gccgcggcgt
      301 cgaaaccgtg cgcaccgcta cgttcgccgc ccgcgatctc ggcgtgaaaa tgctcacgct
      361 ctacgccttc tccgttgaaa actggaagcg tcctcaagac gaggtcggcg ccctcatggg
      421 cctgctcgaa tattacctgc gcaaggaact cgagacgttc gttcgcgatc gcgtccgcct
      481 ccgcaccatc ggccgcacgt ccgacctccc cgccggcgtc caaaaactcc tccgcaccac
      541 catcgaggaa accaaacact tcgcggacta cacgctcgtc ctcgcgctca actacggcgc
      601 acgcactgag atcgtcgacg ccgcccgcgc ctacgccgcc gctgtcgccg ccggcaccga
      661 gaaactcaac gacagctcct gggacacctt tagccgctat ctctacaccg ccgatctccc
      721 cgatcccgat ctcatcatcc gcacgtcggg cgagaaacgc atcagcaatt tcctcctgat
      781 gcagggcgcc tacgccgaat acgtttttac cccggtgctc tggcccgact tcacgaaagc
      841 cgacctcgcc gccgcgatcg cggaatactc ccgccgcgaa cgccgcttcg gccagaccag
      901 cgagcaagtg aagccggccg tcgccgccac caagtagttt ccgtccacgc cccttcggac
      961 tccagcgccg cgtttgcctt tgcttcgcgc gcgcacccgc atcgcgtttc cgccgacgaa
     1021 tctcatccct ttt
//
LOCUS       DASPLT010000267         1654 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_167327, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000267 DASPLT010000000
VERSION     DASPLT010000267.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1654)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1654)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1654
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_167327"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1537)
                     /gene="pstC"
                     /locus_tag="VEA63_02825"
     CDS             complement(<1..1537)
                     /gene="pstC"
                     /locus_tag="VEA63_02825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007360027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HYD82948.1"
                     /translation="MSTESPPAPDRPFVIEKARTRFFGLTLDEIIKAFFGGNALTAVV
                     VLALITLFLFREGADFFGQNRDSITVYRRAGLEFVDIMRRQVEDHTALTRYLFDIRMR
                     AVRHYAEEESLPVAEANARLAAFDDFAGRYSDAVEPMRGLVSELTDVAADIKTRYRIN
                     EDAKVERAQYLAAGMTAEAEAVKIEEIDFEKEVAVLTGITPMYVEAAAGVSVALAEVV
                     AAPPAMPVPELRSRVQRFQALTHEYLGTFPETQRQLETWDYAKPVPFWDTVTGFIFGR
                     EWLTASFWQDWYGVVPLFVGSLMVSIIALALAVPLGVGSAIYINQFATLREQRIVKPA
                     IEFISAIPSVVLGFFGIAVLGQALRAFSQWEAMSWVPGFPFAERLNALTAGCLLGLIA
                     VPTIFTLAEDALNNVPRSFKEASLALGASRMQTIIRIVVPASLSGIVSAVLLGLGRVI
                     GETMVVLLCAGNRIAIPDFSQGLAAFVEPVHTMTGIIAQEMGEVVRGDIHYHALFMVG
                     LVLF"
BASE COUNT          355 a          575 c          472 g          252 t
ORIGIN      
        1 agaagagcac gagtccgacc atgaacagcg cgtgatagtg aatgtcgccg cgcaccactt
       61 cgcccatctc ctgcgcgatg atgccggtca tcgtgtgcac cggctcgacg aacgccgcga
      121 ggccctggct gaaatcggga atggcgatgc gatttcccgc gcacaacagc accaccatcg
      181 tctcgccaat cacgcgcccc agcccgagca gcaccgccga cacgatgccc gagagcgacg
      241 ccggcaccac gatgcgaatg atcgtctgca tgcgcgacgc acccagcgcc agcgacgcct
      301 ctttgaagga tcgcggcacg ttgttgagcg cgtcctcggc cagcgtgaag attgtcggca
      361 ccgcgatcaa accgagcagg cagccagcgg tcagcgcgtt gagccgctcg gcaaagggaa
      421 accccggcac ccagctcatc gcctcccatt gcgaaaacgc gcgcagcgcc tgccccagca
      481 ccgcaatgcc aaaaaatccg agcacgaccg acggaatcgc cgagatgaac tcgattgccg
      541 gcttcacgat gcgctgttcg cgcaacgtcg cgaattgatt gatgtagatt gccgacccga
      601 caccgagcgg caccgccagc gcgagcgcga tgatcgacac catgagcgaa cccacgaaga
      661 gcggcacgac gccataccag tcctgccaaa aactcgccgt cagccactcg cgtccaaaaa
      721 tgaaacccgt caccgtgtcc caaaacggca ccggcttcgc ataatcccac gtctcaagct
      781 gacgctgcgt ttcaggaaac gtgccgagat actcatgcgt cagcgcttga aaccgctgca
      841 cgcgcgatcg cagctccgga accggcatcg ccggcggcgc cgccacgact tccgccagcg
      901 ccaccgagac gcccgccgca gcttcgacat acatcggcgt gatgccggtc aacaccgcga
      961 cctctttctc gaaatcgatc tcctcgatct tcaccgcctc ggcttctgcg gtcattcccg
     1021 ccgcgagata ctgcgctcgt tccaccttcg cgtcctcgtt gattcgataa cgcgttttga
     1081 tgtcggccgc cacgtcggtg agttccgaaa cgagaccgcg catcggctcc accgcgtcgc
     1141 tgtagcgccc cgcaaaatcg tcgaacgcag ccagccgcgc attcgcctcc gccacgggta
     1201 ggctttcctc ctccgcgtag tgccggaccg cgcgcatccg gatgtcgaaa agataacgcg
     1261 tcagcgcggt gtgatcttcg acctgccgcc gcatgatatc cacaaactcc aaccccgcgc
     1321 ggcgatacac ggtgatgcta tcgcgattct gcccgaagaa atccgcgcct tcgcgaaaca
     1381 gaaacagcgt gatcagcgcc agcaccacga cggccgtgag cgcattgccg ccgaagaacg
     1441 ccttgatgat ctcgtcaagc gtcagcccga aaaaacgcgt gcgcgctttc tcgatgacga
     1501 agggccggtc gggcgccgga ggagactcgg tggacatgca gtttgaagaa aagggcgatc
     1561 tgaagcggaa cgcggtttgg gaatgagaca gaggacggct cgcgggagcg tcaagcaggc
     1621 cgcccgcgac cgcggcccga ggtagcggca ggcc
//
LOCUS       DASPLT010000268         1171 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_146951, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000268 DASPLT010000000
VERSION     DASPLT010000268.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1171)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1171)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1171
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_146951"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1171)
                     /locus_tag="VEA63_02830"
     CDS             complement(<1..>1171)
                     /locus_tag="VEA63_02830"
                     /inference="COORDINATES: protein motif:HMM:NF039587.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF5916 domain-containing protein"
                     /protein_id="HYD82949.1"
                     /translation="FNTRRTGYRFETNLNGVRHEALYTTVNSFQSDWVTIWDTQTSRD
                     ENGWNAEIEIPFKSLPFDPTTQEWGFNFGRGIRRRGEEMAWVSYNRGFNASVLGTMTG
                     MSGMNQGVGLDIVPSFSVTSQKRHALNVTDYDEQPSLDAFYRITPSMNAALTINTDFS
                     ATEVDNRQVNLTRFNLFFPEKRDFFLNDSDLFQFGNISNVGNNAVAAASRENARPYFS
                     RKIGLGPGGVPVDVEYGGRISGRLGRFNLGTLAIRQGEFGAVDAQSLFIARASANVFA
                     ESNIGFILTDGDPTSNRDNTVAGIDFRYLNTRLASGRQIEGDAWFQQSDTPGLNSDDA
                     AFGFGLRSPNNTGLRAGFGYKEVQRNFNPAMGFVNRTNIEDLFADIGYTHFFGGGA"
BASE COUNT          230 a          356 c          350 g          235 t
ORIGIN      
        1 gcgccgccgc cgaagaaatg cgtgtagccg atatcggcaa acagatcttc gatgttcgtg
       61 cgattgacaa agcccatcgc cggattgaaa ttccgctgca cttccttgta accaaagccg
      121 gcgcgcaggc cggtgttgtt cggcgaacgc agtccaaagc cgaaggccgc atcgtcgctg
      181 ttcaaaccag gcgtgtccga ctgctgaaac cacgcatcac cttcgatctg gcgcccgctc
      241 gcaagccgcg tgttgaggta cctgaagtcg atgccggcaa ccgtgttgtc gcgattcgac
      301 gtcggatcgc catcggtaag aatgaaaccg atgttgcttt cggcgaacac gttcgccgac
      361 gcgcgcgcga tgaacaggct ctgcgcatcg acggcgccga actcaccctg ccggatcgcg
      421 agcgtgccga gattgaagcg gccaagacgg ccgctgatgc gtccgccata ttcgacgtcc
      481 accggcacgc cgccgggacc cagtccgatc ttgcgtgaaa aatacggacg cgcgttctcg
      541 cgcgacgcag ccgccaccgc gttgttgccc acgttgctga tgttgccgaa ctggaacagg
      601 tcggaatcat tcaggaagaa gtcgcgcttc tccggaaaga aaagattgaa cctggtcagg
      661 ttcacctgcc ggttgtcgac ttcggtggcg gaaaaatcgg tgttgatcgt cagcgccgcg
      721 ttcatcgacg gcgtaatgcg gtagaacgca tcgagcgagg gctgctcgtc gtaatcggtc
      781 acgttcagcg catggcgctt ctggctcgtc acggagaacg acggcacgat gtcgaggcct
      841 acgccctggt tcatgccgct catgcccgtc atcgtgccaa gcacgctcgc gttgaagccg
      901 cggttgtacg agacccatgc catttcttca ccgcggcgcc ggatgccgcg cccgaaattg
      961 aagccccact cctgtgtcgt cggatcgaac ggcagcgact tgaatggaat ttcgatctcg
     1021 gcgttccagc cattttcatc gcggctcgtt tgcgtatccc agatcgtcac ccagtccgac
     1081 tggaacgaat tgacggtggt gtacagcgct tcatgccgca cgccattgag gttcgtttcg
     1141 aagcgatagc cggtgcggcg cgtgttgaag g
//
LOCUS       DASPLT010000269         2358 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_64462, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000269 DASPLT010000000
VERSION     DASPLT010000269.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 2358)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2358)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2358
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_64462"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1210)
                     /locus_tag="VEA63_02835"
     CDS             complement(<1..1210)
                     /locus_tag="VEA63_02835"
                     /inference="COORDINATES: protein motif:HMM:NF044745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HYD82950.1"
                     /translation="MKSTRRAFLTQTSLAGGALMAAGVLRGATPVATGSASGERKPVF
                     NMCGYAAPGLERVRIGVIGIGARGSSAVRRLALIEAVELKALCDVVPDKIARAQKELA
                     STAHQPVAYSGGENAWKEMAARDDLDLIYICTPWDLHTPMAVFAMEHGKHAAVEVPAA
                     TTLEECWQLVETSERTRKHCMMLENCCYDFFELLTLNMARQGYFGDIVHGEGAYLHDL
                     MALNFSKTDYYELWRLRENFRNGNLYPTHGLGPICQVLDINRGDRMDYLVSVSTDDFQ
                     MEKRAKELAEKDSTYAPFAGKKFRGNMNTTTIRTVKGRTIMLQHDVTSQRPYSRLHTV
                     VGTKATAQKYPLPARISKGHEWVSAEEFKKLEEAYTFELVKTVGDRAREVGGHGGMDF
                     LMDWRLIDLLR"
     gene            complement(1461..2054)
                     /gene="tsf"
                     /locus_tag="VEA63_02840"
     CDS             complement(1461..2054)
                     /gene="tsf"
                     /locus_tag="VEA63_02840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009514101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HYD82951.1"
                     /translation="MSVTITATMVNDLRAATGAGLLDCKKALTEANGNVDEATTILRK
                     KGAATAAKKADRATNQGLIESYIHVGGKVGVLLEVNCETDFVARNEDFKQFCKDVCLQ
                     IAAANPLYVSRDQVPEADLAKEREIAAAQVQGKPPAAVQKIVEGKLDKFYSTICLLDQ
                     PFVKLPEKTVKDILTEKITKIGENLQIRRFTRYQLGA"
     gene            complement(2064..>2358)
                     /locus_tag="VEA63_02845"
     CDS             complement(2064..>2358)
                     /locus_tag="VEA63_02845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012377286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HYD82952.1"
                     /translation="DDAVKSIRIIVEAIVAAVQSGLSQRDSRRAARGAADVKSAAAAS
                     AGIGAEQPAVASDEIDLSKVELPPDVVPLVEGEAEVVAPKKITRPKRAVVKAE"
BASE COUNT          421 a          725 c          714 g          498 t
ORIGIN      
        1 tgcgcagcag gtcgatcaag cgccagtcca tcaggaaatc cataccgcca tgtccgccga
       61 cctcccgcgc gcgatcgccg actgttttca cgagctcgaa tgtgtaagct tcctcgagct
      121 tcttgaactc ctcggcggaa acccactcgt ggcccttcga gatgcgcgcg ggcaacggat
      181 acttttgcgc ggtcgctttc gtgccgacga cggtgtggag gcgcgaataa ggccgctgac
      241 tcgtcacgtc gtgctgcaac atgatcgtgc ggcccttcac ggtgcgaatc gtcgtcgtgt
      301 tcatgttgcc acgaaatttt ttcccggcga acggcgcgta agttgaatcc ttttcggcga
      361 gttccttcgc tcttttttcc atctgaaaat cgtccgtcga aaccgacacg aggtagtcca
      421 tgcgatcgcc tcgattgatg tcgagaacct ggcaaatcgg cccgagtccg tgcgtcggat
      481 aaagatttcc gttgcggaaa ttctccctca agcgccacag ctcgtagtaa tcagtcttgc
      541 tgaaattcag cgccatcaag tcgtgaaggt aagcgccttc gccgtgcacg atgtcgccaa
      601 aatatccctg ccgcgccatg ttcaatgtca gcagctcgaa gaaatcgtag cagcaattct
      661 cgagcatcat gcagtgcttc cgcgtgcgct ccgaggtttc gacgagttgc cagcattcct
      721 ccaacgtggt ggcggccggc acctcaaccg cggcgtgctt gccgtgctcc atcgcgaaca
      781 ccgccatcgg cgtgtggaga tcccacggcg tgcagatgta gatcagatcg aggtcgtcgc
      841 gcgcggccat ttccttccac gcgttttcgc cgccgctgta agccacgggc tggtgcgcgg
      901 tcgacgcgag ttccttctgc gctcgagcga tcttgtccgg gacaacatcg cagagcgcct
      961 tcaactccac cgcctcgatg agcgcgagcc gtcgcaccgc gctggagcct cgcgcgccaa
     1021 tgccgatcac accgatccgc accctctcga gccccggcgc ggcatagccg cacatgttga
     1081 aaaccggttt cctctcgccg cttgcgcttc ccgtcgcaac cggtgtcgcc ccacgcaaca
     1141 cgcccgccgc catcaatgcg ccgccggcca gactcgtttg ggtgagaaac gctcgacggg
     1201 tcgatttcat cgctcgaaca gccaacgaaa cgaaatctca ctgcgcgaca gaatttttgc
     1261 ccgcggctct gccacgcgca cgcgtgccga aaagccggcg ctgacgatcc gagttttctg
     1321 cgcttcggga attatcagac gcgtatcagc gcggggctgc aagcaggctc ctgccgttgg
     1381 aagcgaagcc aaaaaaaagc gcctcgatta acgaggcgct tgaagtgttt cgaagaaacg
     1441 aagcgatgcc tgatcagcga tcaggcgccg agttggtaac gcgtgaagcg gcggatctgc
     1501 agattctcgc cgatcttcgt gatcttttcg gtgagaatgt ccttcaccgt tttttcgggc
     1561 agcttcacga acggttgatc gagcaaacag atcgtggagt agaacttgtc gagcttgcct
     1621 tcgacgatct tctgcacggc cgccggcggc ttgccttgga cctgagcggc agcaatctcg
     1681 cgctccttcg cgaggtcggc ctcggggacc tgatcgcgtg aaacgtagag cgggttcgcg
     1741 gcggcgatct gaagacagac gtctttgcag aactgcttga agtcctcgtt gcgggcaacg
     1801 aagtcggtct cgcaattgac ctcgaggagc acgccgactt tgccgccgac gtggatgtag
     1861 ctctcgatca ggccttggtt ggtggcccga tcggctttct tcgcggcggt ggcggcgcct
     1921 ttcttgcgca ggatcgtggt cgcttcgtcg acgttaccgt tggcttcggt gagcgctttt
     1981 ttgcagtcga gcaacccggc gccggtggcg gcgcgcaggt cgttgaccat ggtggcggtg
     2041 atggtgacgg acattgttga aacttattcg gctttgacga ccgcgcgctt cgggcgggtg
     2101 attttcttgg gcgcgacgac ctcggcttcg ccctcgacaa gaggaacgac atccggcggc
     2161 agctcgactt tcgacagatc gatttcgtcg gacgcgacgg caggctgttc ggcgccgatg
     2221 ccagcggacg cggcggcggc ggacttgacg tcggcggcac cgcgggcggc gcggcggctg
     2281 tcgcgctgcg agagaccgct ttgcacggcg gcgacgatcg cctcgacgat gatgcggatg
     2341 gatttcaccg cgtcgtca
//
LOCUS       DASPLT010000270         2474 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_146965, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000270 DASPLT010000000
VERSION     DASPLT010000270.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 2474)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2474)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2474
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_146965"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..715
                     /locus_tag="VEA63_02850"
     CDS             <1..715
                     /locus_tag="VEA63_02850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD82953.1"
                     /translation="QALMNALANSQVDHDNNPGTPAIAFKNYSGLKVINPYMSGGHTG
                     IRDPNSTKLYQMSGYSRANLVAAAQVEIDSARTYFIAGASPVPSDRYVQFGFWKVTDT
                     TASPPLWEHVADQLYSAYNGATPTAVGFFMENLAATKTAQDADPADGTPVPSYGEALL
                     YTNDFAYTGFQALGNFARPFNPNHVYKTVNGSPGASMEYAYNTYRARYFEVYISDLQS
                     PTFQLELKRWRDFLAALPN"
     gene            complement(1008..>2474)
                     /locus_tag="VEA63_02855"
     CDS             complement(1008..>2474)
                     /locus_tag="VEA63_02855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4350 domain-containing protein"
                     /protein_id="HYD82954.1"
                     /translation="RLDQFLGWCREADLTVQPSLFVGWMSGGVFWPKWKAGRNAFSDS
                     FMRRRAVALAAAAARVCARHGDIVLAMDQGNELCCLPDCSAAAPEEVAAWCAEVNAAI
                     AREFPTALRISGNEQTQVIADSGWRLGAQPGCDLYSMHTYPNSAWHPLRFDGMTDPLG
                     QSLFPFYLKCARAFGPVMAQEFGTLFTAGKEAADYLRAILPACHAAGANGYLWWCLRD
                     IAARVHPYEKNAFEGGMGLVDAAGRVKSALRPFLEFARDVTETPADDVRPPDVALYWP
                     KHYYPRDNPLNPGNEPRVLSRRMIVAHFALEQLGLRVGVERGDLPTGNAGANTIVIAG
                     ALLSDSEVRALTGWVRAGGRLVWHGLEATTWGRATDELTGATAVDFLAPRAEGVEAFG
                     ETWNFREFARDVRVGVRPTTAWVVAKDRSGRAVVLENRLGRGGVAVCLAQPDDGFADE
                     SEDRAARSRWSRWYAGMLKCCGHALPSGIARKEVRAAD"
BASE COUNT          473 a          958 c          625 g          418 t
ORIGIN      
        1 ccaggcgctg atgaacgcgc tggcgaacag ccaggtcgat cacgacaaca accccggcac
       61 cccggcaatc gcgttcaaaa attattccgg cttgaaggtg atcaatcctt acatgagcgg
      121 cggccacacc ggcatccgcg atcccaacag caccaagctg tatcaaatgt ctggatatag
      181 ccgcgcgaat ctcgtcgccg ccgcgcaggt cgagatcgac tccgctcgca cctatttcat
      241 cgccggcgcg tctcccgtcc cttccgatcg ctacgtccag ttcggtttct ggaaagtcac
      301 cgacaccacc gccagtcctc cgctctggga acacgtcgcc gatcaactct actcggccta
      361 caacggagcc acgcccaccg ccgtcggttt cttcatggaa aatctcgccg ccaccaaaac
      421 ggcgcaggac gccgaccccg ccgacggcac gcccgtcccg agctacggcg aagcgctgct
      481 ctacacgaac gacttcgcct acaccggctt tcaggcgctc gggaacttcg ctcgcccgtt
      541 caaccccaac catgtctaca agacggtcaa cggcagcccc ggcgcgtcga tggaatacgc
      601 ctacaacacc tatcgcgctc gctacttcga agtctacatc tccgacctcc agtcaccaac
      661 cttccagctc gagttgaaac gctggcgcga ctttctcgcc gcgctgccca actgacgcgc
      721 atcccgttca cgcgcctgga tcgactctcc accgatccgg gctttcggcg cttctgagcc
      781 gcttgaaatc actctcgacc cttcactgcg gcccgccgaa gcgatccacg atgtttcgat
      841 ttctcgcttt ccttctattt ccgatgtttc gccgatgctc ttcagaaaac tggcctcggt
      901 ttcacgtcac ttggtcgaag gtgccaaaca cggcgcaccg gaatacggcg ccaacgacat
      961 ccccgcctgg gtcgaagtct cttcggcgaa cctctccaaa cagtaaacta gtccgcggcg
     1021 cggacctcct tccgcgcaat tcctgaaggc aatgcgtggc cgcagcattt cagcatgcct
     1081 gcataccagc gcgaccaacg cgaccgcgcc gcccgatcct ccgactcgtc ggcaaaccca
     1141 tcgtccggtt gcgccagaca aaccgccact ccgccgcgcc ccaaccggtt ttccaggacc
     1201 accgcgcgtc cgcttcggtc tttcgccacc acccacgccg tcgtaggccg cacgcccacc
     1261 cgcacgtcgc gcgcgaactc gcgaaaattc caggtttcgc cgaaggcttc gacaccctcc
     1321 gcccgcggcg ctagaaaatc gaccgcggtc gcacccgtga gttcgtccgt cgcccgaccc
     1381 caagtggtcg cctccaaccc gtgccacacc aatcggccgc cggcacgcac ccagccggtc
     1441 aacgccctca cctcgctgtc gctcagcagc gcgccggcga tcacgatcgt attcgccccg
     1501 gcattacccg tcggcagatc tccgcgctcc acccccactc gaagcccaag ttgctcgagc
     1561 gcgaaatggg ccacgatcat ccgccgcgat agcacccgcg gttcgttccc gggattgagc
     1621 ggattatcgc gaggatagta gtgcttcggc caatacagcg ccacgtcggg cgggcgcaca
     1681 tcatccgccg gtgtttccgt cacatctctc gcgaactcca gaaacggccg tagcgccgac
     1741 ttcacgcgtc ctgctgcatc caccaacccc attccgcctt cgaacgcatt tttctcgtag
     1801 ggatgcaccc gtgccgcgat gtcgcgcagg caccaccaca aataaccgtt ggccccggcc
     1861 gcatgacacg ccggcagaat cgcgcgcaga tagtccgccg cctctttccc cgccgtgaag
     1921 agcgtgccga attcctgcgc catcaccgga ccgaacgccc gcgcgcattt caaatagaag
     1981 ggaaacagac tttgtccgag cggatcggtc atgccgtcga accgcaacgg atgccacgcg
     2041 ctgttcggat acgtgtgcat cgaatacaaa tcgcagcccg gctgcgcgcc cagccgccag
     2101 ccggagtccg cgatcacctg cgtctgctcg ttgcccgaaa tccgcagcgc tgtcgggaac
     2161 tcccgggcga tcgccgcgtt cacctccgcg caccacgccg ccacctcctc cggcgccgcg
     2221 gccgagcaat ccggcaggca acacagctcg ttgccctggt ccatcgccag cacgatgtcg
     2281 ccgtgccgcg cacagacgcg cgccgccgcc gccgcgagcg ccaccgcccg ccgccgcatg
     2341 aaggaatcgg aaaacgcgtt tcgccccgct ttccactttg gccagaaaac gccgccgctc
     2401 atccacccga cgaacagcga cggctgcact gtcagatccg cctcgcgaca ccagccgaga
     2461 aattgatcca gccg
//
LOCUS       DASPLT010000271         1645 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_269376, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000271 DASPLT010000000
VERSION     DASPLT010000271.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1645)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1645)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1645
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_269376"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..790)
                     /locus_tag="VEA63_02860"
     CDS             complement(<1..790)
                     /locus_tag="VEA63_02860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family peptidase"
                     /protein_id="HYD82955.1"
                     /translation="MRFDKRAAPQVFAVMAANPFLDPVFHIRWSALTPPDIEPAIASA
                     LAGAQAAIDRIATQDMAGLGYENTFLALEHATEELTQTWGKVTHLQSVADSPELRAAH
                     NAVLPQVSAFYARIPLNAELWQRLKAFAESPAGRALGGIHRRFVDETTAEFRQAGADL
                     PPEKRGRLEALQTELSQLTQKYSENVLDATNAWQLIVEDEAKLAGLPAHAKAAARRGA
                     EAKGLAGWRFTLHQPSQEPFMVYLEDAALRRELWSAAVAVGAAAP"
     gene            complement(795..>1645)
                     /locus_tag="VEA63_02865"
     CDS             complement(795..>1645)
                     /locus_tag="VEA63_02865"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HYD82956.1"
                     /translation="YRTRTMGKISLVPEKTWALEDGADLIAKAGVAYDASNREDGAFS
                     PIFEIAREQRSATVQRVYFSYAEATQVPTYTALKSSPSAGLFRGNQNLGRSTSRNIEA
                     GLRANVGGWAAEAALFHRFDDDLVDWTFSRGVTARAANAVDMETTGIELVARRSWTPL
                     DLVLGYTWLTKEADYRNAPVDASFYALNYAEHRLTLAIVARLSRSLELRVDNEARIQA
                     GNLLRTRGGERPLLTAVGVTYRPAAWRRVSLTAQADNLWDSEFQEVPAVPASPRQISG
                     IVGYTW"
BASE COUNT          284 a          606 c          473 g          282 t
ORIGIN      
        1 gcggcgccgc cgcgccgacc gccaccgccg cggaccacag ctcacgccgc agcgccgcgt
       61 cttccagata aaccatgaac ggttcctgcg acggctggtg cagcgtgaac cgccagcccg
      121 cgagcccctt cgcctccgca ccccgacgcg ccgctgcctt cgcgtgcgcc ggcaagcccg
      181 cgagcttcgc ttcatcctcg acgatcagtt gccacgcatt cgtcgcatcg agcacgttct
      241 ccgaatattt ctgcgtgagt tgcgacagct ccgtctgcaa ggcctccaac cgcccgcgtt
      301 tctccggcgg caaatccgcg cccgcctggc gaaattccgc cgtcgtctcg tcgacaaatc
      361 gccggtgaat tcccccgagc gcgcgtcctg ccggcgattc cgcaaacgct ttaagccgct
      421 gccacagttc cgcattcagc gggatgcgcg cgtaaaacgc cgacacctgc ggcagcactg
      481 cattgtgcgc cgcgcgcagc tccggcgagt cagccaccga ttgcaggtgc gtgaccttgc
      541 cccacgtttg cgtgagttct tccgtcgcgt gctcgagagc gagaaacgtg ttctcgtagc
      601 cgagccccgc catgtcctga gtcgcgatcc gatcgatcgc cgcctgcgcg cccgcgagcg
      661 ccgacgcgat cgccggctcg atgtccggcg gtgtcagcgc ggaccagcgg atgtgaaata
      721 caggatcgag aaacgggttt gcggccatca ccgcaaacac ctgcggcgcc gcgcgcttgt
      781 caaaccgcaa aacgttacca cgtgtagccg acgattcccg aaatctgccg cggcgacgcc
      841 ggcaccgccg gcacttcctg aaattccgag tcccacaaat tatccgcctg cgccgtcagc
      901 gacacccgcc gccacgccgc cggccgatac gtgacaccca ccgccgtgag caacggacgc
      961 tcgccgccgc gcgtgcgcaa cagattcccc gcctggatgc gcgcctcgtt gtcgacacgc
     1021 agttccaacg agcgactcag ccgcgcgacg atcgcgagcg tcaaccgatg ctcggcgtag
     1081 ttcagcgcgt agaaactggc gtcgaccggc gcgtttcgat aatcggcctc cttcgtcagc
     1141 cacgtgtaac ccaacacgag atcgagcggc gtccacgatc gacgcgcgac cagttcgatg
     1201 ccggtcgtct ccatatcgac ggcatttgcc gcgcgcgccg tgacgccgcg gctaaacgtc
     1261 cagtcgacaa gatcgtcgtc gaatcggtga aacagcgccg cctccgccgc ccatccgccg
     1321 acgttcgcgc gcaggccggc ctcgatattc cggctcgttg aacgtccgag attttggttt
     1381 ccgcgaaaca gtcctgcact cggactcgac ttcagcgccg tgtaagtcgg cacctgcgtg
     1441 gcttccgcgt agctgaaata cacgcgctgc acggtcgccg agcgctgctc gcgcgcgatc
     1501 tcgaaaattg gtgaaaacgc gccgtcttcg cgattcgagg cgtcgtaggc cacgcccgct
     1561 ttcgcgatca agtccgcgcc gtcttcaagc gcccaagttt tctcgggcac cagggaaatc
     1621 ttgcccatcg tccgcgtgcg atacg
//
LOCUS       DASPLT010000272         1039 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_249129, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000272 DASPLT010000000
VERSION     DASPLT010000272.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1039)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1039)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1039
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_249129"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..372)
                     /locus_tag="VEA63_02870"
     CDS             complement(<1..372)
                     /locus_tag="VEA63_02870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD82957.1"
                     /translation="MKTPTKPKPIPAVRFRALLSLAMAAASGPALAAQVDNENEEVVT
                     LPEFTVSTSSEQGYIVPNAISGGRGGERITETPFSVQALTNEFMNDFQLFEMADQLRF
                     IAGAFSGAEDTGTNNGKTLRGF"
     gene            complement(402..>1039)
                     /locus_tag="VEA63_02875"
     CDS             complement(402..>1039)
                     /locus_tag="VEA63_02875"
                     /inference="COORDINATES: protein motif:HMM:NF019583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase domain-containing protein"
                     /protein_id="HYD82958.1"
                     /translation="VAKLDRMLALPPRFETGRYSHEIHEMTEMAAVDFGQYAHSNEPV
                     HHVLYLYTYAGRPDRTQHWVRRILDELYTPMRFPGDEDNGAMSAWYVLSALGFYPVAV
                     GHPAYVLGAPRFPRATLHLPNGRTFVIAADGDVDAACYTARVTLNGSDHASLELPHRA
                     ITDGGNLTFHLTDQPALAAARGRLPAPFSLSTAPRPHARQAALRPTRAAAG"
BASE COUNT          181 a          279 c          394 g          185 t
ORIGIN      
        1 gaagccgcgg agcgtttttc cgttgttcgt gccggtgtct tctgcgccgg aaaacgcgcc
       61 cgcgatgaag cggagctggt cagccatttc aaacaactga aagtcgttca tgaattcgtt
      121 cgtcagcgct tgcacggaga acggcgtttc cgtgatgcgt tcgccgccgc gtccgccgga
      181 aatggcgttg ggaacgatat agccctgctc cgaactggtg ctgacggtga actccgggag
      241 cgtgacgact tcttcatttt cgttgtcgac ttgagcagcg agagcggggc cggaggcggc
      301 cgccatcgcg agtgagagca gtgcgcggaa gcgcaccgcc gggatcggtt ttggtttggt
      361 tggggttttc acaggcaaaa ggggttcgag aggattgggc gtcagccggc ggcggcgcgg
      421 gtcggtcgca acgcagcttg gcgtgcgtga ggccgtgggg cggtcgagag cgaaaacggc
      481 gccgggaggc gtccgcgcgc cgcggcgagc gcgggctgat cggtgaggtg aaacgtcagg
      541 tttccgccgt cggtgatcgc gcggtgcggc agctccaagc tggcgtgatc ggagccgttc
      601 agcgtgacgc gcgcggtgta acacgcggcg tcgacgtcgc cgtcggcggc gatgacgaac
      661 gtgcgaccgt tgggcaggtg aagtgtggcg cgcgggaagc gcggagcgcc gagaacgtaa
      721 gccggatggc cgaccgcaac cggatagaat ccgagcgcgc tcaagacata ccaagccgac
      781 atcgcgccgt tatcctcgtc gccggggaaa cgcatcggcg tgtagagttc gtcgagaatg
      841 cgccgcaccc agtgctgcgt gcgatcgggc cggccggcgt aggtgtagag atagagaacg
      901 tgatgaacgg gctcgttgga gtgagcgtat tggccgaagt cgacggcggc catttcggtc
      961 atctcgtgga tttcgtgcga gtagcggccg gtctcgaagc gcggcggcaa cgcgagcatg
     1021 cgatcgagct ttgcgacga
//
LOCUS       DASPLT010000273         2511 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_3639, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000273 DASPLT010000000
VERSION     DASPLT010000273.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 2511)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2511)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2511
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3639"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..899)
                     /locus_tag="VEA63_02880"
     CDS             complement(<1..899)
                     /locus_tag="VEA63_02880"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HYD82959.1"
                     /translation="MKFRPFGKHAFNCSEIGFGGWAIGGSWGNQADTDSLAALHRALD
                     LGVNFIDTAAGYGDGRSERLIAQVLRERASDGKKEKVFVATKTPPAEGIWPPSPYCRA
                     EERYSEDYLGENIAIRCANLGVEQLDLLQLHTWTRAWNRNPTPFKILRTLQAEGKIGL
                     VGVSTPEQDQNSVIDLMRGGWVDSVQVIYSLFEQEPAAELLDVAKECGVGVIVRVVFD
                     EGVLTGKFTADTQFAPDDFRAKYFEGDRLARAVAHAEEVHKDLEGSSYTMAQAAIKFV
                     LAHPAVTTVIPGIRNVAQAEANC"
     gene            complement(1189..2061)
                     /locus_tag="VEA63_02885"
     CDS             complement(1189..2061)
                     /locus_tag="VEA63_02885"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HYD82960.1"
                     /translation="MLRYFAHGPVRWKNGMRCNTRTNWEFYAVTSGQCGLRFSDRQRP
                     TLHHRKLWVFAPECSHAWADHRGRDYTRLSLHFGSVPYPLDEITRQNGGWLEKDLEPA
                     HIERLAAIATQLEPEFLHPNSLSPLLFQRALIDLSLLVLEGRVVDLLPPALTDLANFK
                     IERAISWILEHLPQNPTVKQVADAIHVSPSHLRRLFWLSRRSSPKAVFRRLRLDRAKE
                     LMSRSAMTLDDVAHHCGYSSASHLCRDCRDVHHFSPTTWRKKLVDRFTRPLPAGMTPV
                     REYSARPEERTMPA"
     gene            complement(2304..>2511)
                     /locus_tag="VEA63_02890"
     CDS             complement(2304..>2511)
                     /locus_tag="VEA63_02890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD82961.1"
                     /translation="LFVPNQPTFLVAGRTLVNVGISYRADRWTARIQVNNALDKDYIL
                     AAGSRTAAVPGDPRNVRASFTYNF"
BASE COUNT          457 a          662 c          913 g          479 t
ORIGIN      
        1 ctgcagttgg cctcggcttg agcgacgttg cggatgccgg gaatcacggt ggtaacggcg
       61 gggtgcgcga ggacgaactt gatcgcggct tgcgccatcg tgtagctcga gccctcaagg
      121 tctttgtgga cctcttcagc gtgcgccacg gcgcgggcga ggcggtcgcc ttcgaaatat
      181 ttcgcgcgaa agtcgtcggg agcaaactgc gtgtcggcgg tgaatttgcc ggtgagcacg
      241 ccttcgtcga agacgacgcg cacgatgacg ccgacgccgc actccttcgc gacgtcgagg
      301 agttcggcgg cgggctcttg ttcgaagaga ctatagatca cctgcacgct atcgacccag
      361 ccgccgcgca tgagatcgat cacgctgttc tggtcttgct cgggcgtgga gacgccgacg
      421 agtccgattt ttccttcggc ttggagtgtg cgcaggattt tgaacggagt gggattgcga
      481 ttccaggcgc gcgtccaggt gtggagttga aggagatcga gttgttcgac gccgagattg
      541 gcacaacgta tggcaatgtt ttcgccgagg tagtcttcgc tgtagcgctc ctcggcgcga
      601 cagtagggcg aaggcggcca gatgccttcg gccggcggag ttttcgtcgc gacgaatacc
      661 ttttcttttt tgccgtcgga ggcgcgctcg cggaggactt gcgcgatcag gcgttcgctg
      721 cgtccgtcgc cgtagccggc ggcggtgtcg atgaagttga cgccgagatc gagtgcacga
      781 tgcagcgcgg cgagcgaatc ggtatcggct tggttgcccc aggatccgcc gatggcccag
      841 ccgccaaacc cgatttcaga gcagttgaac gcgtgtttgc cgaagggacg aaatttcatg
      901 gagcgatgaa cttgagccgc ggaaccgcgg ttgtcagctc agcctttcgc gcgcacggcg
      961 ccatcagagc gacggctcgg cgcgtggcgg cgagccggcg tcgcgctaag cgggactcat
     1021 gcagaagaga aaccacgaat ggacacgaat gaacacgaac tcagaggaac cgaagagaag
     1081 ctctcggccg gatcgttcca tgacgatttg gtgttccgat cctcattcga actcgatttc
     1141 attggtatcc gttcgtgtcc attcgtggtt caaatgaatt gttatggctt acgcgggcat
     1201 cgtgcgttcc tcggggcggg cgctgtattc acgcacgggc gtcatgcccg cggggagcgg
     1261 acgcgtgaag cgatcgacga gttttttgcg ccaggtcgtc ggcgagaaat gatgcacgtc
     1321 gcggcaatcg cggcagaggt gactcgcgct ggagtaaccg cagtgatgcg cgacgtcgtc
     1381 gagcgtcatc gcggagcggc tcatgagctc cttggcccgg tcgagtcgca gtcggcggaa
     1441 aaccgctttc gggctcgagc ggcgcgaaag ccagaacagc cggcgaaggt ggctcgggga
     1501 tacgtgaatg gcgtcggcga cttgcttgac ggtaggattt tgaggcaggt gttcgaggat
     1561 ccaggagatc gcgcgctcga tcttgaagtt ggcgagatcg gtgagcgccg ggggaagcaa
     1621 gtcgacgacg cggccctcga ggacgagtag cgaaaggtcg atcagcgcgc gttgaaacag
     1681 cagcgggcta agcgagttgg gatggagaaa ttccggctcg agctgcgtgg cgatggcggc
     1741 gagacgttcg atgtgtgccg gttcgagatc tttctcgagc cagccgccgt tttggcgggt
     1801 gatttcgtcg agcggataag gaacgctgcc gaagtgaagg gagaggcgcg tgtagtcgcg
     1861 tccgcggtgg tcggcccacg cgtgcgaaca ttcgggagcg aagacccaga gcttgcggtg
     1921 gtgaagcgtc ggccgctggc ggtcggagaa gcgaaggccg cactgtccgc tcgtgacggc
     1981 atagaattcc cagttcgtgc gcgtgttgca gcgcatgccg tttttccagc gaacaggacc
     2041 gtgagcgaag tagcgcaaca tgggggcggc cgtccgatct tcttgggggg aactctaggg
     2101 ctactcggcg cgggggcgcg cacaagcggc agaggacttg aacagtcaga atgggctgaa
     2161 gtgccaaaga aaagggccgg cgttttcacg ccggcccgaa ggcggggatg cgcttgcgcg
     2221 cggagtcggg gcgcaaagca ctcccacggt tgttttttta cggtcagcgc ggcgggggcg
     2281 ccgcggctcc aagagcgtcg cgactagaag ttataggtga acgaggcgcg gacgttgcgc
     2341 gggtcgccgg gaacggcggc ggtgcggctg ccggcggcga ggatgtagtc cttgtcgagg
     2401 gcgttgttga cttgaatccg tgcggtccag cggtcggcgc gatagctgat gccgacgttc
     2461 acgagggtgc gaccggcgac gagaaacgtc ggctggttcg gcacgaagag a
//
LOCUS       DASPLT010000274         3057 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_187441, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000274 DASPLT010000000
VERSION     DASPLT010000274.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 3057)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3057)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3057
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_187441"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..970
                     /locus_tag="VEA63_02895"
     CDS             <1..970
                     /locus_tag="VEA63_02895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009512590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HYD82962.1"
                     /translation="GRTVRVAFNYRWAPGATKVREVLAAGTIGRIRQVTLEYLLNTSH
                     GADYFRRWHAQKRNSGGLLVHKSTHHFDLVNWWIDGIPNEVVAHGGLLFYGRRNAVAR
                     GDQALTTYPRYYGHPVARDPFAYDYSHSMLQDPAMEKALYLGAEQETGYVRDQNVFRD
                     DIDIEDTLNVLVRYRDGTLLNYSLNAYSPYEGYRVTFTGDRGRIEYRELHGAHIVGNG
                     VVGAEHLGAEEPELRVFPHFKPAYQVEVPRLEGAHGGGDPLLQEQIFSRNPPNDPFQR
                     AAGHEQGAASILVGVAANQSIATGQPVALPSLVALRPAAVHLSDLQ"
     gene            999..1907
                     /locus_tag="VEA63_02900"
     CDS             999..1907
                     /locus_tag="VEA63_02900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD82963.1"
                     /translation="MLPQRLRPLLACTLLALASLSASLFGADAERLTLIEEPGVFTIH
                     YSGKSGYIGIPSWKAVVSRDDGGNITALHVPADHPVPLSSRTGQWPISILMSKNTLGI
                     DGTMNKGRENFARFPVEVFKLVEHSSDKIVIAVGGPSPNRHYEHHRTYTFTPAGVQIE
                     GSVLPLMDLSSVAFDPHFNRTQIADSHLAALPLRTQGRGGWVYMPSSGRDGATALPEG
                     VDFPLEAQLRLRRSEPIFLKIFYDKNFDAAEGKRLLIHNNKDGWEARADRVIFEKLVG
                     IVGFPVPKGAQQTFKVRFEFETQPWE"
     gene            2055..>3057
                     /locus_tag="VEA63_02905"
     CDS             2055..>3057
                     /locus_tag="VEA63_02905"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HYD82964.1"
                     /translation="MKAVSSRARANILRRLFAIACAFILASGSVASAALFPTDFPTAP
                     PEAHGFDPQKLDALWTSLEQRQTNVFVVMRNDRIVYERYANGYTRTKPHYTASLAKAL
                     VSGLSLMLAIDDRLIDPADRASRYVPQWQTVPRKQDITIAQLVTHTSGLEDANVDGVP
                     HRELTGWKGDFWRQLPEPNDPFTLSRDQSPVLFPPSSQYHYSNPGIAMLTYCVTAALR
                     DAPQKDIRSLLKHRIMDPLDIPENEWDVGYDRTYNVDGLPLVGSWGGAGFSANATARI
                     GRMLLHKGDWDEKTIFSQEIVAAARRSAGQPSNAGLGWWINIRPDFTRVFPTAPADSF
                     "
BASE COUNT          550 a         1115 c          827 g          565 t
ORIGIN      
        1 cggccgcacc gtccgcgtcg ctttcaacta ccgctgggcg ccgggcgcga cgaaggtccg
       61 cgaggtgctc gccgccggca cgatcggccg cattcgccag gtcacgctgg agtatctgtt
      121 gaacaccagc cacggcgccg actacttccg ccgctggcac gcccagaagc gcaactccgg
      181 cggtctcctc gtgcacaagt cgacgcacca cttcgatctc gtgaactggt ggatcgacgg
      241 cattcccaac gaggtcgtcg cccacggcgg cctgctgttc tacggccgcc gcaacgccgt
      301 cgctcgcggc gatcaggccc tgaccactta cccgcgctac tacggccacc ccgtcgcccg
      361 cgatccgttc gcctacgact acagtcattc gatgctgcag gatccggcca tggagaaggc
      421 gctctatctc ggcgcggaac aggaaaccgg ctacgtgcgc gatcagaacg tgtttcgcga
      481 cgatatcgac atcgaggata cgctcaacgt tctcgtgcgc tatcgcgacg gcacgctgct
      541 caactactcg ctcaacgcct acagtcctta cgaaggttat cgcgtcacct tcacgggcga
      601 ccgcggccgg atcgaatatc gcgagctgca cggcgctcac atcgtgggca acggcgtcgt
      661 cggcgccgaa catctcggcg ccgaggagcc cgagctgcgc gtctttccgc atttcaagcc
      721 ggcctaccaa gtggaagtgc cgcgcctcga aggcgcgcat ggtggcggcg atccgctgct
      781 gcaggaacag attttttccc gcaacccgcc caacgatccg tttcaacgcg ccgcgggaca
      841 cgaacagggc gccgcgtcga ttctcgtcgg cgtcgccgcc aatcaaagca tcgccaccgg
      901 ccagcccgtc gctctcccta gcctcgtcgc gttgcggccc gccgccgttc acttgtccga
      961 tctgcaataa cctccagtcg actccgcgcc tttcccctat gttaccccaa cgccttcgcc
     1021 ccctgctcgc ctgcaccctc ctcgctctcg cttctttgtc cgcctcgttg ttcggcgccg
     1081 acgccgagcg gctcacgctg atcgaggagc cgggtgtatt cacgattcat tacagcggaa
     1141 aatccggcta catcggcatt ccttcctgga aagcggtggt gagtcgcgac gacggcggaa
     1201 acatcactgc gctgcacgtg cccgcggatc atcccgtgcc gctctcgtcg cgcaccggcc
     1261 agtggccgat cagcatcctg atgtcgaaaa acacgctcgg catcgacggc acgatgaaca
     1321 agggccgcga aaacttcgcg cgatttcccg tcgaagtttt caaactcgtc gaacactcct
     1381 ccgataaaat cgtgatcgcc gtcggcggtc cgtctccaaa ccggcactac gaacatcacc
     1441 gcacctacac cttcacgccc gcgggcgtgc agattgaagg cagcgttctg cctttgatgg
     1501 atctgtcatc cgtcgcgttc gatcctcatt tcaaccgcac ccagatcgcc gactcccatc
     1561 tcgctgcatt gccgcttcgg acgcagggcc gcggcggctg ggtttatatg ccgtcgagcg
     1621 gccgcgacgg cgccaccgct ttgccggagg gcgtcgactt tccactcgaa gcccagctcc
     1681 gcctgcgccg cagcgaaccg attttcctga agatttttta cgacaagaat ttcgacgccg
     1741 ccgagggaaa gcgtctgttg atccacaaca acaaggatgg ttgggaggcc cgagccgacc
     1801 gcgtcatttt cgaaaaactc gtcggcatcg tcggctttcc cgtccccaag ggcgcacaac
     1861 agacgttcaa ggttcgcttc gaattcgaaa cccagccgtg ggagtgatcg ccgccgacag
     1921 gcgcgagcta tgagaggggc tttacgctcc ggctcccgcc gcccctctcc tcgccgcacc
     1981 gtttcacaaa cgccctcgct gtcaccacaa tctcactacg caacttcgcc catatcgtcg
     2041 gcgtcgttaa tcgcatgaaa gcagtctctt ctcgcgcccg cgccaatatc ctccgccgcc
     2101 tcttcgccat cgcctgcgcc ttcatcctcg ccagcggctc cgtcgccagc gccgcgcttt
     2161 ttcccaccga cttccccacc gctccgcccg aagcccacgg cttcgatccc caaaaactcg
     2221 acgcgctctg gaccagtctc gagcagcgtc agaccaatgt attcgtcgtg atgcgcaacg
     2281 accgcatcgt ctacgaacgc tacgccaacg gctacacgcg cacgaagccg cactacaccg
     2341 cgtcgctcgc gaaagcgctc gtcagcggcc tctcgctcat gctggcgatc gacgaccgcc
     2401 tgatcgatcc ggcggatcgc gcgagccgct acgtgccgca gtggcagacc gttcctcgca
     2461 agcaagacat caccatcgct caactcgtca cgcacacctc gggcctcgag gacgccaacg
     2521 tagacggcgt tccccaccgc gaactcaccg gttggaaagg cgatttctgg cggcagcttc
     2581 ccgagcccaa cgatccgttc accctctccc gcgaccagtc gccggtgctt tttccgccca
     2641 gctcccaata tcactacagc aatcccggca tcgcgatgct gacgtattgc gtgaccgccg
     2701 cgctgcgcga tgcgccgcag aaagacatcc gctcgttgtt gaagcatcgg atcatggacc
     2761 cgctcgatat tccggagaac gaatgggacg tcggctacga tcgcacttac aatgtagacg
     2821 gcctgccgct cgtcggttcg tggggcggcg ccggcttcag cgccaacgcc acggcgcgca
     2881 tcggccgcat gctgctgcat aaaggcgatt gggatgagaa aacgatcttc tcccaggaaa
     2941 tcgtcgccgc cgcgcggcgc tccgccggcc agccgagcaa cgccggtctc ggctggtgga
     3001 tcaacatccg ccctgatttc acgcgcgtgt ttcccaccgc cccggcggat tcgttct
//
LOCUS       DASPLT010000275         1012 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_147011, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000275 DASPLT010000000
VERSION     DASPLT010000275.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1012)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1012)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1012
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_147011"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..752)
                     /locus_tag="VEA63_02910"
     CDS             complement(<1..752)
                     /locus_tag="VEA63_02910"
                     /inference="COORDINATES: protein motif:HMM:NF019259.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1592 domain-containing protein"
                     /protein_id="HYD82965.1"
                     /translation="MPGGASAVIGLNQIEVQGPFNPAGLSSTPARAKIFTCYPNVAAE
                     EEACAREIIGRNARLAYRGTATEHDVERLTRIYAQSSAAEGFEMGVRHALTAIIASPK
                     FFYREESLPENAVAGEIYPVTDLELASRLSFFLWSSIPDAELLDLAEAGRLHDSAVLA
                     QQVDRMLRDPRATTLASNFASQWLNLAKLDEIDPDPDLFRDVGSRVRNMFRKEIELLA
                     GDVFLGNKPVTELMTADYTYLNESLALHYGIH"
     gene            792..>1012
                     /locus_tag="VEA63_02915"
     CDS             792..>1012
                     /locus_tag="VEA63_02915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD82966.1"
                     /translation="MDERHGELVWTRGCRELDVFQLCIHFVDGWIADLVDGFQVGAAA
                     NVHFEKALAVECGNHGVREFLVRDPQCGG"
BASE COUNT          182 a          300 c          319 g          211 t
ORIGIN      
        1 ctgtggatgc cgtaatgcag cgcgagactt tcgttaaggt acgtgtaatc ggccgtcatc
       61 agctccgtga cgggtttgtt gccgaggaac acgtcgcccg cgagcaactc gatttccttg
      121 cggaacatgt tgcgcacgcg cgagccgacg tcgcggaaca gatccggatc gggatcgatc
      181 tcgtcgagct tggcgagatt cagccactgt gacgcgaaat tggaggccag cgtcgtcgcg
      241 cgcggatcgc gcagcatgcg atccacctgc tgcgcgagca cggcagagtc atgtagccgg
      301 ccggcttcgg ccagatccag caattcggcg tctggaatgc tgctccagag gaagaacgac
      361 aggcgcgacg cgagttcgag gtcagtcact gggtaaattt caccagccac ggcattctcg
      421 ggcagcgatt cctcgcgata gaaaaacttc gggctggcga tgattgccgt cagcgcatgg
      481 cgtacgccca tttcgaaacc ttcggccgcg ctgctttgcg cataaattct cgtcaggcgc
      541 tccacatcgt gttcagtagc cgtgccgcga tacgcgagcc tcgcgttacg gccgatgatc
      601 tcgcgcgcgc aggcttcttc ttccgcggcc acgttcggat agcacgtgaa aattttcgcg
      661 cgtgccggcg tcgagctgag gccggccgga ttgaacggac cctgtacttc gatctggttc
      721 aggccgatga ccgccgacgc gccgcccggc acctgcggcg acagcgtgcc ttcgaattcg
      781 gcgaacgagc ggtggatgaa cgtcacggcg agcttgtgtg gacccgcggt tgccgtgaac
      841 tcgatgtttt tcagctttgc attcatttcg tcgacggctg gatcgccgat ctggtcgatg
      901 gctttcaggt tggcgccgcc gccaacgtcc atttcgaaaa agcgctcgcc gtcgagtgtg
      961 gcaatcacgg tgtgcgtgaa ttcctggttc gtgacccaca atgcggcggc ga
//
LOCUS       DASPLT010000276         1163 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_147013, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000276 DASPLT010000000
VERSION     DASPLT010000276.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1163)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1163)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1163
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_147013"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..999)
                     /locus_tag="VEA63_02920"
     CDS             complement(<1..999)
                     /locus_tag="VEA63_02920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD82967.1"
                     /translation="MRYFPASLLFLGGLAGASLIHAADAPKTVHRVTAAPYDSDVARE
                     RELYEVTPYPFSPDMVLEGGGIDVLPDGRLVVSTRRGELWLVDDAFADDFTKARFTLF
                     ATGLHEPLSVFFKDGWFYVTERNGITRVRDSEGDDRADVFEIVTDQFGVTGNFHEYAF
                     SSRPDREGNIWAALCLANASTSPVPWRGWVLRVTPEGKIIPTAAGVRSPGGVAFNAEG
                     DCFLTDNQGDWNGSSSLKHVPPGSFQGTPPSLDWWDRAGAELGPRPLDPSSGRILTDR
                     LADPKFIPPAVLIPHNRIGRSPTAIAFDTSGKFGPFGQQLFVAEHTFSQITRISLEK"
BASE COUNT          212 a          334 c          427 g          190 t
ORIGIN      
        1 tttctcgagc gagatgcggg tgatttggga aaacgtgtgt tcggcgacga agagctgctg
       61 gccgaacggg ccgaattttc cggaggtgtc gaacgcgatc gcggtcggcg agcggccgat
      121 gcggttgtgc gggatgagca cggcgggcgg gatgaatttc ggatcggcga ggcggtcggt
      181 gaggatgcgg ccgctcgacg gatcgagcgg cctcgggccg agttcagcac ccgcgcgatc
      241 ccaccagtcg aggctcggcg gcgtgccttg gaaagaaccg ggcggcacgt gcttcagcga
      301 cgatgaaccg ttccaatcac cctggttgtc ggtgaggaaa caatcgcctt cggcgttgaa
      361 agcgacgccg ccaggtgagc gcacgccggc ggcggtcggg atgatttttc cctcgggcgt
      421 cacacgcagc acccagccgc gccacggcac gggactcgtg ctggcgttgg cgaggcagag
      481 cgcggcccag atgttgccct cgcgatcggg tcgcgagctg aaggcatatt cgtggaaatt
      541 tccggtgacg ccgaactggt cggtcacgat ctcgaacaca tcggcacgat catcgccctc
      601 actgtcgcgc acgcgcgtga tgccgtttcg ctcggtgacg tagaaccagc cgtctttgaa
      661 aaacacgctg agcggttcgt gcagaccggt agcgaacaac gtgaaccggg ctttggtgaa
      721 atcgtccgcg aacgcgtcgt cgacgagcca gagctcgccg cgacgcgtgg acacgacgag
      781 gcggccgtcg ggcaggacgt cgatgccgcc gccctcgagc accatgtcgg gagaaaacgg
      841 atacggcgtg acctcgtaga gttcgcgctc acgcgcgacg tccgagtcgt aaggcgcggc
      901 cgtgacgcga tgaacggttt tcggggcgtc cgccgcgtgg atgagcgagg ctccggcgag
      961 tccgccgaga aaaagaagcg aggcaggaaa ataacgcatg ttcggaagat cagtgagcgt
     1021 gggcggcggc ggctttcgcc gcggtggcgg gccagcggta ggtgacgatc aacgtggcgc
     1081 gttcgccgtt gaatgggacg cgggcgatcc attcgcgaga gcctgaggcg tcgcgcagat
     1141 cgccggcgct gccctcgagt gta
//
LOCUS       DASPLT010000277         3657 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_64535, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000277 DASPLT010000000
VERSION     DASPLT010000277.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 3657)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3657)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3657
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_64535"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1802)
                     /locus_tag="VEA63_02925"
     CDS             complement(<1..1802)
                     /locus_tag="VEA63_02925"
                     /inference="COORDINATES: protein motif:HMM:NF024215.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HYD82968.1"
                     /translation="MLPRLSSPALRGFLERESTRPDLGRATRATFAVTIPLLLAAAGA
                     LPLSVMFVALVGQNIAMVDVRGSYALRIGLLLAMTAILTGAAGLGAWASHSTLLAIGV
                     VGLVAAAGGVWRHLTPDYGASLSISSMLVVLLSVSAPPADDLIGHHALSALIGGLWGV
                     LLQIVNWPINPQHPLRRTVADSWVAVADLFAALAPGSAERSQHIQECESQLRTTLDHT
                     YRVLALAKPSKLRARLEELNLAAARLATRVVALNTALETALADPASAWLADSLRPVLT
                     SLTNTSRSVAVLLVSRQPAHLAAFEVRLRRLTNLLRAFQLETPARLGDPATAAHLREI
                     LRQIEFHLPNIHALLRGTVDRADERAAFSLELFDLQTWTLRPLASALHFHRHVDPALV
                     RFTVRLTVLTMLGVAAFKYWNLPHGFWLPLTMAIVLQPDYGSTRERAAQRVLGTLAGS
                     LAATVLLWPQLPFAVLTAATSATIFAFAYFLKRRYAVAVFFITLFVVLLTEAHEPITL
                     WFTLERIASTLVGGAVALLAALLFWPVWERDRLPPVLAAALDANRDYLQRIAARLSAG
                     GAYDGPVIVAKRQAETANSAVFSSLQRMIADPRNR"
     gene            complement(1811..2890)
                     /gene="mnmA"
                     /locus_tag="VEA63_02930"
     CDS             complement(1811..2890)
                     /gene="mnmA"
                     /locus_tag="VEA63_02930"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HYD82969.1"
                     /translation="MAAPEKILVAMSGGVDSSVAALLLKQQGYDVVGAYMKNWINEDE
                     VIGHCPWMQDIEDARRVTDQIGIEFRVVNLMHEYRERVVAYLLDGYARGLTPNPDIMC
                     NREIKFGVFRAWAKDHGFSAVATGHYARRAAGPGEFALLEGADKNKDQSYFLALLSQD
                     QLRDARFPIGHLPKPELRRLAREAGLATAEKKDSQGICFIGEVKMQDFLRAYVPDAPG
                     PIVRASDSRELGTHRGLHFYTLGQRKGIGVPSNTDHQAYVVVGKRAADRALLVAFDHP
                     DAPGLFQREVRVHALSWIGEPLDSHRELEGRVRYRDPRVPLRFTPEGGGTALIAFRDP
                     QRGLASGQILAFYEGERLLGGGVYV"
     gene            3031..>3657
                     /locus_tag="VEA63_02935"
     CDS             3031..>3657
                     /locus_tag="VEA63_02935"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HYD82970.1"
                     /translation="MRRSLFVWVFAMAVAGATVAQAQFAVAVRKPERPIPAIERVLVI
                     SVDGLRPDRALLANMPTLRAMLHDGAYTFWAKTTVVAITLPSHTSMVTGVTPNKHGIS
                     WNHDLPLSQPVYPNQPTLMEMAKKVGYTTAMVAGKSKFDTLGKPGTLDYMFVPEKGTV
                     GNDVVLAEALKVIEQHKPEVLYLHFAEVDSTGHRHGWGSAEQFAAIERT"
BASE COUNT          684 a          998 c         1401 g          574 t
ORIGIN      
        1 cggttgcgcg gatcggcgat catgcgttgc agcgacgaga agacggcgct gttcgcggtt
       61 tccgcctggc gtttcgcgac gatcaccggt ccgtcgtagg cgccgccggc cgacaaacgc
      121 gcggcgatcc gttgcaagta atcgcggttc gcatcgaggg cggcggcgag cacgggcggc
      181 aggcgatcgc gttcccagac cggccagaaa aggagcgcgg cgagcagagc gacagcgccg
      241 ccaacgagcg tgctggcgat gcgttcgagg gtgaaccaca gcgtgatcgg ctcgtgcgct
      301 tcggtgagga gcacgacgaa aagggtgatg aagaaaacgg cgacggcata gcggcgttta
      361 agaaaatacg cgaaggcgaa gatcgttgcg gacgtggcgg cagtgagcac ggcgaagggg
      421 agttgcggcc agagcagcac cgtcgcggca aggctgccgg cgagcgtgcc gaggacgcgt
      481 tgcgcggcgc gttcgcgagt ggagccgtag tcgggctgga gcacgatcgc catcgtgagc
      541 ggcagccaga agccgtgcgg aagattccag tatttgaacg cggcgacgcc gagcatcgtg
      601 agaacggtaa ggcgaacggt gaaacgcacc agcgcgggat cgacgtggcg gtgaaaatgc
      661 agcgcggagg cgagcgggcg aagggtccac gtttgcaggt cgaacagctc gagggaaaac
      721 gcggcgcgtt cgtcggcgcg atcgacggtg ccgcggagca gcgcgtggat gttcggcaga
      781 tggaattcga tctggcgaag aatttcgcgc agatgcgcgg cggtcgcggg atcgccgagc
      841 cgcgcgggcg tctcgagttg gaaggcgcgc agaagattcg tgagccggcg gaggcggact
      901 tcgaaggcgg cgaggtgagc gggttgtcgc gacacgagga gcacggcgac actgcgcgag
      961 gtgttggtga gcgacgtgag cacggggcgc agcgagtcgg cgagccaggc ggacgcggga
     1021 tcggcgagcg cggtttcgag ggcggtgttg agcgcaacga cgcgggtcgc gaggcgggcg
     1081 gcggcgaggt tgagttcttc gagccgcgcg cggagcttcg agggtttggc gagcgcgagg
     1141 acgcgatacg tgtgatcgag cgtggtgcga agctgcgatt cgcattcctg gatgtgctgg
     1201 ctgcgctcgg cggatccggg ggcgagcgcg gcgaaaagat cggcgacggc gacccagctg
     1261 tcggcgacgg tgcggcgaag cggatgttga ggattaatcg gccagttgac gatttggagc
     1321 agcacgcccc acaaaccgcc gatgagcgcg ctgagtgcgt gatgaccgat gagatcgtcg
     1381 gcgggcggag cgctgacgga caacagaacg acaagcatcg acgagataga gagcgacgcg
     1441 ccgtagtcag gcgtgaggtg ccgccacacg ccgcccgcgg cagcgacgag gccaacgacg
     1501 ccgatggcga ggagcgtgct gtgcgaggcc cacgcgccga ggcccgcggc gccggtgagg
     1561 atcgcagtca tcgcgagcag caagccgata cgaagcgcgt aggagccgcg aacgtcgacc
     1621 atcgcgatgt tttgcccgac cagcgcaacg aacatgacgc tcaacggcag cgctccggcg
     1681 gcggcgagga gcagcggaat cgtgaccgca aacgtggcgc gggtggcgcg accaagatcg
     1741 ggtcgcgtcg attcgcgttc gagaaatccg cgcaaggccg gcgatgaaag ccgtgggagc
     1801 atgggggact tcagacgtag acgccgccgc cgagcaggcg ttcgccctcg tagaaggcga
     1861 ggatttggcc gctggcgaga ccgcgttgag gatcgcgaaa ggcgatcagc gcggtgccgc
     1921 cgccttcggg cgtgaaacga agcggcacgc gaggatcgcg atagcgcacg cggccttcga
     1981 gttcacggtg cgagtcgagc ggctcgccga tccagctcaa cgcatgcacg cgcacctcgc
     2041 gttgaaacag gcccggcgcg tcgggatgat cgaaggcgac gaggagcgcg cggtcggcgg
     2101 cgcgtttgcc gacgacaacg taagcctgat gatcggtgtt cgagggaacg ccgatgcctt
     2161 tgcgctggcc gagcgtgtag aagtggagcc cgcgatgcgt gccgagttcg cggctgtcgc
     2221 ttgcgcgcac gatcgggccg ggcgcatcgg gcacgtaggc gcgaaggaaa tcctgcatct
     2281 tcacctcacc gatgaagcag atgccctggc tgtctttttt ttcggcggtg gccaggccgg
     2341 cttcgcgtgc gaggcggcgg agttccggtt tgggaaggtg gccgatggga aaacgggcgt
     2401 cgcgaagctg gtcttgggaa agcaacgcga ggaagtagga ctgatctttg tttttatcgg
     2461 cgccttcgag tagtgcgaac tcgccgggtc ccgccgcgcg gcgggcgtag tggccggtgg
     2521 cgacggcgct gaagccgtgg tctttcgccc aggcgcgaaa cacgccgaat ttgatctcgc
     2581 ggttgcacat gatgtccggg ttcggcgtga gtccgcgcgc gtaaccatcg agcaaatacg
     2641 cgacgacccg ctcgcggtat tcgtgcatga ggttcacgac gcggaactcg atgccgatct
     2701 ggtcggtcac gcgacgggcg tcctcgatgt cctgcatcca cgggcagtgg ccgatgactt
     2761 cgtcctcgtt gatccagttc ttcatgtagg cgccaacgac gtcgtagccc tgctgtttta
     2821 gaagcagggc ggcgacggag ctgtcgacgc caccggacat ggcgacgagg attttttcgg
     2881 gagcggccac ggcgagagac gacgcgggcc ggggaggttt tgtcaaacga agccggcgtg
     2941 gcccagcggg ccagccctac gggatgggaa ttgacgtgtt tgtaatgttc gccgcttgcg
     3001 gtgcgggcgt cgggtttcca gcgtcgccgg atgagacgat ctcttttcgt gtgggtcttc
     3061 gcgatggcgg tggcgggcgc gacggtcgcg caggcgcagt tcgcggtggc ggtgcgaaag
     3121 ccagagcggc cgattccggc gatcgagcgc gtgctggtga tcagcgtgga cgggttgcga
     3181 cccgatcgtg cgctcctcgc gaacatgccg acgttgcggg cgatgttgca cgacggcgcg
     3241 tatacgtttt gggcgaagac gacggtggtc gcgatcacgc tgccgtcgca cacgagcatg
     3301 gtgaccggtg tcacgccgaa caaacacggg atctcgtgga accatgacct gccgttgagc
     3361 caaccggttt atccgaatca accgacgctg atggaaatgg cgaaaaaggt gggctacacg
     3421 acagcgatgg tcgcggggaa gtcgaagttc gacacgctcg ggaagccggg aacgcttgat
     3481 tacatgttcg tgccggaaaa gggcacggtg ggaaatgacg tcgtcctggc cgaagcgttg
     3541 aaagtgatcg agcagcacaa gcccgaagtg ttgtatcttc atttcgcgga ggtcgactcg
     3601 acggggcatc ggcatgggtg gggctcggcg gagcaatttg cggcgatcga gcgaacg
//
LOCUS       DASPLT010000278         1905 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_64591, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000278 DASPLT010000000
VERSION     DASPLT010000278.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1905)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1905)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1905
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_64591"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1269
                     /locus_tag="VEA63_02940"
     CDS             <1..1269
                     /locus_tag="VEA63_02940"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HYD82971.1"
                     /translation="REHGELLALCDLSPARLAHYNSLLAGSLGYRSLPTYSADRFETM
                     LREQRPDVVIVTSKDSTHHDYIVRALRAGCDVITEKPMTIDAAKCRSILDTVADTGRR
                     VRVAFNYRWQAYRTKVKALLATGAIGRVHAVNLEYVLDMRHGVDYYRRWHAHLGDSGG
                     LLVHKSTHHFDLVNWWLDAIPDEVFARGQLDFYGRANAVARGDEALTRYARYTDEPAA
                     AHDPFRLNLRDGGNLESLYLRAEADSGYRRDQNVFRDDIDIYDNMGALVRYRTGALLT
                     YSLVSFSSREGMRVSFTGDRGTLEYHEFLGTHIIRGQSDAELDREQSTADRPVEFIRV
                     YPHFKPSYEVSVERAKGGHGGADPALMENLFSADALPDNYQRAAGHEQGAASILVGIA
                     ANESASRHRPVKLLDLVPLKPSARRLSELV"
     gene            1294..>1905
                     /locus_tag="VEA63_02945"
     CDS             1294..>1905
                     /locus_tag="VEA63_02945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD82972.1"
                     /translation="MNATIPAVAPASAAASPNEAAALSPWTLMLRRFRRHRVAILALY
                     GLAVLYTLALGAELFAPYPREWRNLDRAYNPPQIPRFSFAQGLYVPAMKRVIDPVTFK
                     KSYYEDKAQPIALGFFVRGEPYKLWGLIPADRHFFGVKQPGPVAAEHTFYLLGADKYG
                     RDLCSRLIYGSRISLSVGLVAIAVTFLLGVSIGGVSGYVGGALD"
BASE COUNT          335 a          681 c          515 g          374 t
ORIGIN      
        1 cgcgaacacg gtgaactgct cgcgctgtgc gatctgagcc ctgcccgtct ggcgcattac
       61 aacagcctcc tcgccggttc gctcggctac cgttcgctgc caacctactc cgccgatcgg
      121 tttgaaacga tgctccgcga acagcggccc gatgtcgtca tcgtcacgtc gaaagactcc
      181 actcatcacg actacatcgt tcgcgcgctt cgcgccggct gcgatgtcat caccgagaaa
      241 ccgatgacga tcgacgccgc gaaatgccgc tcgatcctcg acaccgtcgc cgacaccggc
      301 cgccgggtgc gcgttgcctt caactaccgc tggcaagcct accgcaccaa agtaaaagcg
      361 ctgctcgcca ccggcgcgat cggccgggtg cacgccgtca atctcgagta tgtcttggat
      421 atgcggcacg gcgtcgacta ctaccgccgc tggcacgcac acctcggaga ctcgggcggg
      481 ctgctcgtgc acaagagcac gcaccatttc gacctcgtga actggtggct cgatgcgatt
      541 cccgacgaag ttttcgcccg cggccaactc gatttctacg gtcgcgccaa cgccgtcgcc
      601 cgcggcgacg aagcgctgac gcgttacgcc cgctataccg acgagcccgc cgcggcgcac
      661 gatccgtttc ggctcaacct gcgcgacggc ggcaatctcg agtcgctgta tctgcgcgcc
      721 gaagccgaca gcggttatcg tcgcgatcaa aatgtctttc gcgacgatat cgatatctac
      781 gacaacatgg gggcgctcgt ccgctaccgc accggcgcgc tgctcactta ttcgctcgtg
      841 agcttctcct cgcgcgaggg catgcgcgtc agcttcaccg gcgatcgcgg cactctggag
      901 tatcacgaat ttctcggcac gcacatcatc cgcggacaaa gcgacgccga actcgatcgc
      961 gaacaatcga ccgccgatcg cccggtcgag ttcatccggg tctatccgca cttcaaaccg
     1021 agttacgaag tttcggtcga acgcgccaaa ggcggccacg gcggcgccga tcctgcactg
     1081 atggaaaatc ttttttccgc cgacgcactt ccggacaact accaacgcgc cgccggccac
     1141 gaacaaggcg ctgcctcgat cctcgtcggc atcgccgcca acgaatccgc cagcagacat
     1201 cgccccgtga aactgctcga tctcgtgccg ctcaaaccct ccgcgcggcg cctgagcgaa
     1261 ctcgtctgat ccctcgtcac ccgcctgctt cttgtgaacg ccaccatccc cgccgtcgct
     1321 cctgcctccg ccgctgcttc tcccaacgag gccgctgcgc tgtcgccgtg gacgctgatg
     1381 ctgcgacgtt tccggcggca ccgggtcgcc atcctcgcac tgtatggtct ggctgttctt
     1441 tatacgctgg cgctcggcgc cgaacttttt gcgccctacc cgcgtgaatg gcgcaatctc
     1501 gaccgcgctt acaaccctcc gcaaatcccg cggttctcat tcgcccaagg gctttacgtg
     1561 ccggcgatga aacgcgtcat cgatcccgtc acgtttaaaa aatcctacta tgaagacaaa
     1621 gcccagccga tcgctctcgg cttttttgtc cgcggcgaac cttacaaatt gtggggcctg
     1681 attcccgccg accgacattt cttcggcgtg aaacaaccgg ggcccgttgc cgccgaacac
     1741 acgttctatc ttctcggcgc cgacaaatac ggtcgcgatc tctgcagccg tctcatctac
     1801 ggttcgcgca tcagtctctc ggtcggactg gtggcgatcg ccgtgacgtt cctgctcggt
     1861 gtgagcattg gcggcgtgtc cggttacgtc ggtggcgcgc tcgac
//
LOCUS       DASPLT010000279         1433 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_187522, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000279 DASPLT010000000
VERSION     DASPLT010000279.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1433)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1433)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1433
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_187522"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            241..>1433
                     /locus_tag="VEA63_02950"
     CDS             241..>1433
                     /locus_tag="VEA63_02950"
                     /inference="COORDINATES: protein motif:HMM:NF016648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HYD82973.1"
                     /translation="MKIELHRVMPGGYLGCIPDRTPVLAYLTDPAHMKSSTKLRFFSA
                     LCAFAGAIVASAHEANVLKVSGAAEVQLSGQSSAQPLTAGMQVPEGATIKTAAGGQVY
                     LETAPGVVATIESDSTVVVEKLNIEKQGETVILQEALLDLKKGNIISTLDPTKKSINR
                     YGVRTPKGVAAARGTVYGTSVTFSATGESNTTVATMTGVVTLNLGNGVTVDVPFGQAA
                     SGGSEQAEMTTATLEAAIAASGQSGLTVAALLQEAVNAVAGNVAANTSAAGGNDTATA
                     VLAAVVSAASAAQPSQASAFVATAVAAAVTTGSATGGAGSNSAVAAIVEAAVRAAPAS
                     AAGIAQTAASTVVEVKVTEAAAAATAAGGDAAAVAAAVAEASANAEATLQTIAETAVN
                     TSAAVG"
BASE COUNT          272 a          425 c          480 g          256 t
ORIGIN      
        1 tagagggcga cggattttga gtccgttgcg tctgccaatt ccgccatccg ggcgacgcga
       61 gccggccaac gtaggggcga gcgatgactt gtaaatgcga aattccgctg ggtgctggtg
      121 tgtgcgcgca gagtttccca agtctgcacg tggacctcgt ccagttttcg cgttaacgct
      181 tcccgcacat tgccggccag gagggagccg gtagagctcg gcaaggagac cggtggagga
      241 ttgaaaatag aattgcatcg ggttatgccc ggtggctatc tagggtgtat tcctgaccga
      301 acgcctgttt tagcgtatct aaccgatcca gctcacatga aatcctccac taaacttcga
      361 tttttctccg cgctgtgcgc cttcgcaggg gcgatcgtcg cctccgccca tgaggcgaac
      421 gtgctcaagg tctcaggagc ggctgaagtg cagctgtccg gacaaagcag cgcgcagcca
      481 cttaccgccg gtatgcaagt gcctgagggc gcgaccatca aaaccgcagc aggtgggcag
      541 gtctatctcg agaccgcgcc gggtgtcgta gcgacgatcg agagcgattc gacggtggtg
      601 gtggagaagc tcaatatcga gaagcagggc gagactgtta tcctgcagga agcgctcctt
      661 gatctgaaaa agggaaacat catttccacg ctcgatccga caaagaagtc gatcaaccgc
      721 tatggcgtgc gcactccgaa gggagtcgcg gctgctcgcg gaacggttta tggaaccagc
      781 gtcaccttta gtgcgaccgg cgagagcaac acgaccgttg cgaccatgac tggagttgtg
      841 accttgaatc tcggcaatgg agtaaccgtt gacgttcctt tcggtcaggc ggcgagtggc
      901 gggagcgagc aggctgagat gacgaccgcg acgctcgaag cggcgattgc ggcctccggg
      961 caatccggcc tcacggtagc ggctttgttg caggaagcgg ttaacgcggt ggccggcaac
     1021 gtcgccgcca atacgtccgc cgccggcggc aacgacaccg ccaccgccgt gctcgcggcc
     1081 gtggtcagcg ccgcttccgc ggcgcaaccg agccaagctt cggcattcgt ggcaacggcg
     1141 gtcgccgctg ctgtcacaac gggttccgcc accggcgggg ccggaagtaa ttcagcggtt
     1201 gcagccatcg tcgaggccgc ggttcgcgcg gctccggcgt ctgccgcggg tatcgcccag
     1261 acggcggctt cgaccgtcgt cgaggtgaag gtgacggaag cggcagcggc ggctaccgcc
     1321 gctggcggcg atgctgcggc ggtcgcggct gcggtcgccg aagcgtccgc caacgccgaa
     1381 gccactctcc agacgatcgc ggagaccgcg gtcaacacat cggcggcggt tgg
//
LOCUS       DASPLT010000280         1649 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_64596, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000280 DASPLT010000000
VERSION     DASPLT010000280.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1649)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1649)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1649
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_64596"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1649
                     /locus_tag="VEA63_02955"
     CDS             <1..>1649
                     /locus_tag="VEA63_02955"
                     /inference="COORDINATES: protein motif:HMM:TIGR02601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="autotransporter-associated beta strand
                     repeat-containing protein"
                     /protein_id="HYD82974.1"
                     /translation="GAESATIDLDGGILRASANQANFLQGFAAGDMSLGAGGGVFDTN
                     SFAVTVGAVIDGAGALTKRGLGTLTLTAVNTYTGGTRIEGGALAVSAANNLGAAGTAI
                     TLDGGTLQTSGNYSIGRPMTLEEGGGAIGFAPGARLTYTGVISGDGALEQRGSGVLVL
                     TGANTFAGALTLSAGALHIGNGATGSITGDLVNNSWVEFNRTGTLDYAGDISGTGDIV
                     KNLSGTVTLSGALSYEGSTFVNAGTLAIASPSVFASESVTVEGGMLRFEATANAGASE
                     ITVNGGQVRFTGSSSAGSSSITTNTLTFAYFLGDASAGSATITNNGLSFTSFEDNATA
                     DDATIVLGPDAFLDITALTSSGIAIGSLAGEGNVHLGAKRLTVGGLNTSTTLSGNIDG
                     AGGVLEKTGTGTLTLSGENSYTGGTEVEDGTLTIASTDALGTGNVTVYDSVLRFEANA
                     AAGARTITVSDGEVQFAGTSTAEATAMVTASNGVIDLTALTAGGTSIGSISGPGVLHL
                     GDKTLTTGARNALTEFSGVVDGVGGALVMNGIGMLTLSGAN"
BASE COUNT          296 a          461 c          621 g          271 t
ORIGIN      
        1 aggagcggag agcgcgacga tcgatctcga cggcggcatc ttgcgggcgt ccgcgaacca
       61 ggcgaatttc ctgcaaggat tcgcggcagg cgatatgagt ctcggcgcgg gcggcggtgt
      121 tttcgatacg aactctttcg ctgtgaccgt gggtgcggtg atcgacggcg ccggtgcgtt
      181 gacgaagcgg ggactcggca cgctgacact gaccgcggtt aacacctaca cgggcggaac
      241 gaggatcgaa ggcggggcgc tcgcggtcag cgcggcgaac aatcttggcg cggccggcac
      301 ggcaatcacg ctcgatggcg gcacgctgca gacctcgggc aattattcga tcggccggcc
      361 gatgacgctc gaggagggcg gtggtgcgat cggcttcgcg ccgggggcca ggctcactta
      421 cacaggagtc atcagcggcg atggcgcgtt ggagcaacgc ggttcgggcg tgctggtgct
      481 gacaggagcg aacacgttcg ccggggcgtt gactctgagc gcaggggcat tgcacatcgg
      541 caatggcgcg acgggctcga tcaccggcga cttggtgaac aacagctggg tcgagttcaa
      601 tcgcaccggc acgctggatt atgcgggcga tatttccggc accggcgata tcgtgaagaa
      661 cctcagtggc acggtgacgt tgtcgggggc gttgagctat gagggttcga cgttcgtcaa
      721 cgctggcacg ctggcgatcg cctcgccgag cgtgttcgcc agcgagtcgg tgacggtcga
      781 gggaggcatg ctgcgtttcg aggcgacggc gaatgcgggg gcgagcgaga tcacggtgaa
      841 cggtgggcag gttcgcttca cgggttcgtc gtctgcgggc agttcgtcga tcacgacgaa
      901 cacgctcacg ttcgcgtatt ttctcggcga tgcgtcggcg ggttccgcga cgatcacgaa
      961 caacggctta agcttcacgt cgttcgagga caacgcgacc gcggacgacg cgacgatcgt
     1021 cctcgggcca gacgcgttcc tcgatatcac cgcgctcacg agttcgggca tcgcgatcgg
     1081 atcgctcgcc ggcgagggca atgtgcatct cggggcgaag cggctcacgg tcggtgggct
     1141 gaatacgtcg acgacgctca gcggcaacat cgatggggcg ggtggcgtgc tggagaaaac
     1201 ggggaccggg acgctgacgt tgagcggcga gaacagctac accggtggca ccgaggtgga
     1261 agacgggacg ttgacgattg cgtcgacgga cgcgctcggc accgggaacg taacggtcta
     1321 cgacagtgtg ctgcgcttcg aggcgaacgc ggcggcgggc gcgcgcacga tcacggtcag
     1381 cgacggcgaa gtgcagttcg cggggaccag cacggcggag gcgacggcga tggtgacggc
     1441 gagcaatggc gtcatcgatc tcacggcact caccgccggc ggcacgagca tcgggtcgat
     1501 ttcgggcccc ggcgtgttgc acctgggcga caagacgctc acgactggcg cgaggaatgc
     1561 cttgaccgaa ttcagcggcg tcgtcgacgg agtgggtggg gcgctcgtga tgaacggcat
     1621 tggcatgctc acgttgagcg gagcgaaca
//
LOCUS       DASPLT010000281         2164 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_208648, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000281 DASPLT010000000
VERSION     DASPLT010000281.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 2164)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2164)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2164
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_208648"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1218)
                     /locus_tag="VEA63_02960"
     CDS             complement(<1..1218)
                     /locus_tag="VEA63_02960"
                     /inference="COORDINATES: protein motif:HMM:NF021508.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2235 domain-containing protein"
                     /protein_id="HYD82975.1"
                     /translation="MSKLVVCCDGTWNTPDQEEYELPAPTNVLRLYNALALSDGFSPQ
                     YKYYHPGVGTEGSFLKRTAGGMYGAGLAENVQSAYAWLARHYKAGDKIYLFGFSRGAF
                     TVRCLGGMLSRCGILDLRNAVDPFPDEVPSALPVGEAWKRVGTAYEKGYRERKDDWRD
                     ASWELSAPPGVAIELIGVWDTVGALGIPNDLALLNLLDRPKAWSFHDTTLGQNVHYAR
                     HAIAMDEMRASFTPTLWTDTTGRPLHDRSPAADGGPARVRQVFFPGVHSDVGGGYYER
                     GLAEGALQWMIEESAALPGGLVFEPKSTAQLAPDVRAPLHNSLRGIFKLLKTRPRNLP
                     AFSDEDRYSATALSRLKDPPLAQSPYFAPARIVPPGQPPNSPIPPSNVPADRILKRAG
                     ASTRCNIYAVNPWN"
     gene            1500..>2164
                     /locus_tag="VEA63_02965"
     CDS             1500..>2164
                     /locus_tag="VEA63_02965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD82976.1"
                     /translation="MSRPLPLLFLTSLGLAALAPIQAQTPARPAAPVPPWQLSPEERE
                     RLQKLSREDHADMMRQLGITALRPGRNGSTAPGTPNQANYDPAKANPFPDWPDVLTLE
                     SGAKVTTAEQWWKQRRPEIVELFEREIVGRVPGNVPKVTWEVTETINTQVGGLPVVAR
                     RVIGHVDNSAHPEITVDFRMAVVLPVNAKAPVPVLMMFGWGNMPDEPPPRFPNAQEPA
                     APPS"
BASE COUNT          412 a          702 c          671 g          379 t
ORIGIN      
        1 gttccatggg ttcaccgcgt aaatgttaca gcgcgtcgac gcacccgccc gtttgaggat
       61 gcgatcggcg ggcacgttgc tcggtggaat cggcgagttc ggcggctggc ccggcggcac
      121 gatgcgtgcc ggcgcgaaat acggcgactg cgccagcggc ggatccttta accgcgacag
      181 cgccgtcgcc gagtagcgat cctcgtcgct gaaggccggc agatttcgcg gacgcgtttt
      241 caaaagcttg aagataccgc gcagggaatt gtgcagcggc gcgcgcacat cgggcgcgag
      301 ttgcgcggtc gacttcggct cgaacacgag tccgccgggc aacgccgcgg actcctcgat
      361 catccattgg agcgcacctt ccgcgaggcc gcgttcgtag taacctccac cgacgtcgct
      421 gtgaacgccg gggaaaaaca cctgccgcac gcgcgccggc ccgccgtccg ccgccggcga
      481 acgatcatgc aatgggcggc ctgtcgtatc ggtccacaag gtcggcgtga aactcgcgcg
      541 catctcgtcc atcgcgatcg catgacgcgc atagtggacg ttctgaccca gcgtggtgtc
      601 gtgaaacgac caggctttcg ggcgatcgag caggttgagc aaggcgagat cgttcgggat
      661 gccgagcgcg ccaaccgtgt cccaaacacc gatcaactcg atggccactc ccggcggcgc
      721 gctgagttcc caactcgcgt cgcgccagtc gtcctttcgc tcgcggtaac ctttctcgta
      781 agcggtgccg acgcgtttcc acgcttcgcc gaccggcagc gccgaaggga cttcgtcagg
      841 aaacggatcg acggcgtttc gcaaatcgag aatgccgcag cgcgacaaca tcccgccaag
      901 acaacgcacc gtgaacgcgc cgcggctaaa accgaaaagg taaatcttgt cgccagcttt
      961 gtaatgacgc gccagccagg cataggcgct ttgaacattc tccgcgagcc ccgcgccata
     1021 catgccgccg gccgtgcgtt tcaggaagct gccttcggtg ccgacgcccg gatgataata
     1081 cttatattgc gggctgaagc catcgctcag cgcgagcgcg ttgtagaggc gcaggacatt
     1141 ggtgggcgcg ggaagttcgt attcttcctg atcgggggtg ttccaagtgc cgtcacagca
     1201 aacaacgagt ttggacatgg gagagcgggg gttctctaca cagcgagtgg ctcgagctaa
     1261 aaacgcggtg cctaacgcgc ggatgatcga gtgctagtcc gggcgccaac cgcgcagcaa
     1321 tacaattgcg tccgtgtggg aatctttagg ggattggcct ctataagaac cgatgcgcga
     1381 tgcgcagccg tccgtcaggt gacgcgccgt tccgctcgac gttgaatttt ccgcatcgag
     1441 cgccgcgccg gcgcgtcaat gttcatgttc ttccccgaaa ccttggctgc ttacctctca
     1501 tgtcacgtcc cctaccttta ctttttctca cgagtctcgg tctggccgcg ctcgcgccga
     1561 tccaagcgca aactcccgcc cgcccagcgg cgcccgtgcc gccttggcag cttagccccg
     1621 aagaacgcga gcggctgcag aaactcagtc gcgaagatca cgccgacatg atgcgccagt
     1681 tgggaatcac cgcgctgcgt cctggccgaa acggcagcac ggctccgggc acgccgaatc
     1741 aggccaacta cgatccggcg aaagcaaacc cgtttcccga ctggccggat gtcctcacgc
     1801 tcgaaagcgg cgccaaagtc acgaccgcgg agcagtggtg gaagcagcgc cgcccggaaa
     1861 tcgtcgaact cttcgaacgc gaaatcgtcg gccgcgtgcc gggcaacgtg ccgaaggtga
     1921 catgggaggt gaccgaaacc atcaataccc aggtcggcgg attgcccgtc gtcgcgcgac
     1981 gcgtgatcgg ccacgtcgac aactccgcgc atccggagat cacggtcgac tttcgcatgg
     2041 ccgtcgtttt gcccgtgaac gcgaaagcgc cggtgccggt gttgatgatg ttcggctggg
     2101 gcaacatgcc cgacgaaccg ccgccgcgtt ttccgaacgc gcaagagccg gccgctccgc
     2161 cctc
//
LOCUS       DASPLT010000282         1508 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_249254, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000282 DASPLT010000000
VERSION     DASPLT010000282.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1508)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1508)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1508
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_249254"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..293
                     /locus_tag="VEA63_02970"
     CDS             <1..293
                     /locus_tag="VEA63_02970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="histone deacetylase"
                     /protein_id="HYD82977.1"
                     /translation="RNWPIAPRTPRAEHMAALRESLDAVVAFEPTVILISAGFDAYVR
                     DPITAMSLEIEDFATLGRWLRETRLPAAAVLEGGYSEDLPLLIDAFVSAWKK"
     gene            308..1483
                     /locus_tag="VEA63_02975"
     CDS             308..1483
                     /locus_tag="VEA63_02975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HRDC domain-containing protein"
                     /protein_id="HYD82978.1"
                     /translation="MISRFLPLSVRRLLGVKVTSRPPVYTLIDQPGQLAPLLAALDRV
                     DEVSLDTEADNMFHYRTRVCLLQFLVGKEIFLVDSLAPLQLDGLWSRLAEKHLVMHGS
                     DFDLRLLHDFCRFRPKSIFDTMLAAQLINRPRIGLASLLEDHFGVKLSKESQKANWSK
                     RPLTQKMLDYAALDVFYLPALRDILTRELVKLRRLEWLEQQCRAQIESGSIGFAPPDE
                     NDWRIGRSERLRGAGVGVLHAVWHWREQTAQRLDVPPFKVCSSDLLLKIASAAESGES
                     EATILAQVHLGRRHDRLAPSLAAAIRAGLARDPKSLPRRRGRDPTHVALTQSEVERLD
                     RIKDDRDKVAAKLQLEPTLIANRAQLAQIARAPTRLDELLLPWQAELLRNVPSLKTE"
BASE COUNT          258 a          553 c          402 g          295 t
ORIGIN      
        1 gccgcaactg gccgattgcg ccgcgcacgc cccgagccga acacatggcc gccctccgcg
       61 agtcgctgga cgccgtcgtc gccttcgaac cgacagtcat tctcatttct gctggtttcg
      121 atgcctatgt gcgcgatccg attacagcta tgtcgttgga aatcgaagat ttcgccacgc
      181 tcggtcgctg gcttcgcgag actcggctcc ccgccgccgc cgtcctcgaa ggcggttata
      241 gtgaagacct tcctttgttg atcgacgcct tcgtttcggc gtggaagaaa taacgccggt
      301 tttccgcatg atttcgcgct ttcttccgct gtccgttcgc cgtttgcttg gcgtcaaagt
      361 gacttcccgt ccgcccgttt acacccttat cgatcaaccc ggtcagctgg ctcccttgct
      421 cgccgccttg gatcgtgtcg acgaggtgtc cctggatacg gaagcggaca acatgtttca
      481 ctaccggacg cgcgtctgcc tgctccagtt tttggtcggc aaggagatct tcctcgtcga
      541 ctcgctcgcg ccgctccagc tcgacggcct ttggtcccgg ctcgccgaga aacacctcgt
      601 catgcacggc agtgatttcg acctgcgcct cctgcacgac ttctgccgct tccgcccgaa
      661 aagcatcttc gacacgatgc tcgcggccca gctcatcaac cgcccgcgca tcggcctcgc
      721 ttcacttctc gaagatcact tcggcgtaaa actttccaag gaatcgcaaa aagccaactg
      781 gtcgaagcgc ccgctcacgc agaagatgtt ggattacgcc gcgctcgacg tgttttacct
      841 gccggcgctc cgcgacattc ttacccgtga actcgtaaaa ctccgccggc tcgaatggct
      901 cgaacagcaa tgccgcgcgc agatcgaatc gggctccatc ggcttcgcgc cgcctgacga
      961 aaacgactgg cgcatcggcc gcagcgaacg cctccgcggc gccggagtcg gcgtgcttca
     1021 cgccgtctgg cactggcgcg aacaaaccgc tcagcggctc gacgttccgc cgttcaaagt
     1081 ctgcagcagc gacctccttc ttaaaatcgc cagcgccgcc gagtccggcg aatccgaagc
     1141 cacgatcctc gcgcaagttc acctcggccg ccgccacgac cgtctcgccc cgtcgctcgc
     1201 cgccgccatc cgcgccggcc tcgcgcgcga cccgaaatcg cttccccgcc gccgcggccg
     1261 tgatccgacc cacgtcgcgc ttacgcaatc cgaggtggag cgtctcgacc gcatcaaaga
     1321 cgatcgcgat aaagtcgccg cgaagctcca gctcgaaccg acgctgatcg cgaaccgcgc
     1381 gcaactcgcg caaatcgccc gcgcgcccac ccgcctcgac gaactcctgc tcccctggca
     1441 ggccgagctg ttgcgcaacg ttccttcatt gaaaaccgag tagggcgggc ttcagcccgc
     1501 cttggccg
//
LOCUS       DASPLT010000283         1446 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_3749, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000283 DASPLT010000000
VERSION     DASPLT010000283.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1446)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1446)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1446
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3749"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..510
                     /locus_tag="VEA63_02980"
     CDS             <1..510
                     /locus_tag="VEA63_02980"
                     /inference="COORDINATES: protein motif:HMM:NF024240.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HYD82979.1"
                     /translation="LAMAPYIRESRRIRAVTTIREQDVSATHRPGETLAERYEDSVGI
                     GYYRIDLHPSTGGDNYVDVPSLPFRIPLGALLPVRVENLLPAAKNIGTTHITNGCYRL
                     HPVEWNIGEAAGLLASYCIREKIRPREVHQSKDRTRAFQKWIADQGVEIAWPENLNLD
                     HGDPHVHAR"
     gene            531..>1446
                     /locus_tag="VEA63_02985"
     CDS             531..>1446
                     /locus_tag="VEA63_02985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD82980.1"
                     /translation="MPRSPVPLRVFCPRDSHPAVRRAARRLAEPAGEIRDDDVASAQI
                     VVGTAQTLQLPEFPARSFRIWQQGEQLWIAGDRPRSVLAGALYWRHHRARGTLPALPL
                     VRESPYQQRLILEDFPFHCYAPTGFAFDREAYAENLVALGFTAMECNRFSERQPLEPY
                     YANYLFTNPSVAPFVWTRWHAQVWDESIVRANAAELRACVQTALEHDLDPSITSFLPR
                     PFPEKFFRAHPHLRGPLFRHDFLVRGSHAGVRCIDTDHAEGLAFYEEVYRELLRANPE
                     IRHFFFWHADLGTKFWPDGEGSLQCTLTK"
BASE COUNT          248 a          489 c          419 g          290 t
ORIGIN      
        1 ctcgcgatgg cgccttatat ccgcgaatcc cggcgtattc gcgccgtcac taccattcgc
       61 gaacaggatg tctcggccac gcatcggccg ggagaaaccc tcgccgagcg ctacgaagat
      121 tccgtcggca tcggttatta ccgaatcgac ctgcatcctt ccaccggcgg cgacaactac
      181 gtggacgtgc cgtcgcttcc gttccgcatt ccactcggag cgctgctgcc cgtgcgcgtg
      241 gaaaacctgc tccctgcggc gaagaacatt ggcacgaccc acatcaccaa cggctgctac
      301 cgccttcacc ccgtcgagtg gaacatcggc gaagcggcgg ggttgctggc gagctactgt
      361 atccgcgaaa aaatacgccc gcgcgaggtg caccagagca aggaccggac gcgcgcgttc
      421 caaaaatgga tcgccgatca aggcgtggaa atcgcctggc cggagaatct gaacctcgac
      481 cacggcgatc ctcacgtgca cgcgcgttaa ctcttcgacg ccagtcgccc atgccccgct
      541 ctcctgtgcc gcttcgggtc ttctgccctc gcgattcgca tcccgccgtt cgacgcgccg
      601 cgcgtcgcct ggcggagcct gcgggggaaa ttcgcgacga cgacgtggcg tcggcgcaga
      661 tcgtcgttgg cacggcgcag acgctacagc ttccggaatt cccggcgcgt tcgtttcgga
      721 tttggcagca aggtgaacaa ctctggatcg ccggcgaccg gccgcgctcc gtgctcgccg
      781 gcgcactcta ctggcggcat caccgcgcgc gcggcacgct tccggcgctg ccgctggttc
      841 gcgaaagtcc ttatcagcag cggctgattc tcgaggactt tccgtttcat tgctatgcgc
      901 cgacgggctt cgcgttcgat cgggaagctt atgccgagaa tctcgtcgca ctgggtttca
      961 ccgcgatgga gtgcaaccgc ttttccgagc ggcagccgct ggagccctac tacgcgaatt
     1021 atcttttcac caacccgtcc gtcgcgccgt tcgtctggac gcgctggcat gcccaagtct
     1081 gggacgaatc gatcgttcga gccaacgccg ccgagttgcg cgcgtgcgtg caaaccgcgc
     1141 tcgagcacga tctcgatcct tcgatcacca gttttctgcc gcgcccgttt ccggaaaaat
     1201 tctttcgtgc acatcctcat ctgcgagggc cgttgtttcg tcacgatttc cttgtccgtg
     1261 gcagccatgc cggcgtccgc tgcatcgata ccgatcacgc ggaagggctc gcgttctatg
     1321 aagaggttta ccgcgaactg ctgcgcgcga atcccgagat ccgtcatttc ttcttttggc
     1381 acgccgatct cgggacgaaa ttctggcccg acggcgaagg ctcgctgcag tgcacgctca
     1441 cgaaac
//
LOCUS       DASPLT010000284         1139 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_208720, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000284 DASPLT010000000
VERSION     DASPLT010000284.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1139)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1139)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1139
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_208720"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..970
                     /locus_tag="VEA63_02990"
     CDS             <1..970
                     /locus_tag="VEA63_02990"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HYD82981.1"
                     /translation="LLGTGGIFVSLMLPAFFPSMVTLYFTASIIGLAFMGLQLATQTL
                     TGAISSPSERARNFGLLSLGFALANLGGPLLTGYLIDQIGYAWAFAAIATPLVPAIII
                     SAFGSRWIPKVQAKGEAVRGGMFDLLKIKPLRDTLIASGIVSAAWDVYQFFMPLYGRS
                     LGLSATVIGVIMSAFAVSIILVRVVLPLVLRLAGEVQLLTYAMFVAAAAFAIFPLMEG
                     AWALAGASFLLGLGCGLGQPLSMTLVYNASPKGRAGEATGMRITVNQVTHFVIPLLFG
                     AVGSLAGYAVVFLTNAGGLVIGGYTSLRNNAKRAREAETNAKRPQA"
BASE COUNT          179 a          358 c          366 g          236 t
ORIGIN      
        1 cctgctcggc accggcggca tatttgtatc gctgatgctg cccgcattct ttccatcgat
       61 ggtgacgctg tatttcaccg cgtcgatcat cggcctcgct ttcatggggc tgcagctcgc
      121 gacgcagacg ctgacgggcg caatctcaag cccaagcgag cgggcgcgca attttgggct
      181 gctcagtctc gggttcgcgc tcgcgaatct cggcggcccg ctcctcaccg gctatctgat
      241 cgaccagatc ggttatgcgt gggcgttcgc ggcgattgcg acgccgctcg tgccggcgat
      301 catcatctcc gcgttcggtt cgcgctggat accgaaagta caggcgaaag gcgaagcggt
      361 gcgcggcggc atgttcgacc tgctcaagat caagcctctg cgagacacgc tgatcgcgag
      421 cggtatcgtg tctgcggcgt gggatgtcta ccagttcttc atgccgcttt acgggcgctc
      481 gctcggcctt tccgcgacgg tgatcggcgt catcatgagc gcgttcgccg tctcgatcat
      541 tctggtgcgc gtggtcttgc cgctggttct gcgtcttgcg ggtgaagtcc agctcctgac
      601 gtacgcgatg tttgtcgccg cggcagcgtt cgcgattttc ccgctgatgg aaggcgcgtg
      661 ggcgctcgcg ggcgcatcgt tcctgctcgg actcggctgc gggctggggc agccgttgtc
      721 gatgacactg gtgtacaacg cttcgcccaa aggccgcgca ggggaggcga cgggcatgcg
      781 catcaccgtc aaccaggtga cgcacttcgt cattccgctc ctcttcggcg cggtcggttc
      841 gctcgcaggc tacgccgtcg ttttcctgac caacgccggc ggtctcgtca tcggcggcta
      901 cacgagcctg cgcaacaacg cgaaacgcgc acgcgaggcc gagacgaacg ctaagcggcc
      961 gcaagcataa aaactcacga gttcccgttt gatcgtcgcg gccgaggccg cgctgctgac
     1021 gtggcattat ggcaaaaccg tagaggagga gggcaagcca catgaagatc gtattcggac
     1081 cggtcatcgc acccgcagcg tggaccatag gtgagtcgct gctgccccgc ggtttcacg
//
LOCUS       DASPLT010000285         2911 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_64630, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000285 DASPLT010000000
VERSION     DASPLT010000285.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 2911)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2911)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2911
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_64630"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..843
                     /locus_tag="VEA63_02995"
     CDS             <1..843
                     /locus_tag="VEA63_02995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD82982.1"
                     /translation="ELPLESINVYGGTQARVTTNDDAIASYADEMAQGAVFPPIVVYY
                     DGATHWLADGFHRYLAAKRNERPSILAEVEPGGRTDALRHALGANATNGVYRNNADKR
                     NAVEIALEEWPDRANPVIAEICKVSVELVRRTRTQLTQSGKLSASETVTGRDGKTYPS
                     QIERQPRGKTEASSSDRESGGGGGFGGKGKGDGGHLGGTSTELERDARAMIRKGEINP
                     FELPTLLTANADDYAETVINLLGTMKPDDPRRSAGLLRIKQWIEKTLAGEPVAAAVAA
                     ATIA"
     gene            1065..>2911
                     /locus_tag="VEA63_03000"
     CDS             1065..>2911
                     /locus_tag="VEA63_03000"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HYD82983.1"
                     /translation="MLAPCCIVAAEDSAPSPSDASSPTVLHPVTVLGSHIRHTDFEGP
                     SAVRVYDRDYIEATGATRLADFLNLLPQNYGGASSGRGSAPNDLNPEFGVRTETSLPF
                     FNFVLGMAAIPFGQTGVSAANLHGLGSGSTLVLVDGHRRPIAGQGNLSTDTRQGFVNL
                     NTIPLGMIERVEILADGASALYGSDAIGGVINVVLKKNYSGAEFVTDYKGTFEGGGRE
                     RSVSLIAGFTSDDARLRGTIGIDLYDRAPLTAAQRQLSADQDHSGRVIDHDAEGDPIH
                     GIDFRLNWSYPAVVQARTGTLNGVTRPDGSPATVALVPDGARAGLTPADFIVSPSTRA
                     ESARRMNTAAFISLISPQESRSVSGQFEYRLGENLRAYGGASVGEVDTQFDGQPPVSS
                     ASASTGFGSYRTIVPAIIDGQPNTLNPFGQDVLVGLVHHEFGPTRQTTQTISYGAYSG
                     LSGRFADWEWDVGVNWSRERFEQRNLTLDLQKFSDSLAASDPAQRFNPFVDARTAGPV
                     NAHLYEAMKTYDVRHGRSNYAAGSFLAFGPLRELPGGDLQLAVGGNLNTASHRAESRL
                     ANGPTNSVRVERDSFAGFAEAVVPLFGPPNARPLLRRLELQLAVRYEHH"
BASE COUNT          533 a         1110 c          795 g          473 t
ORIGIN      
        1 gagctccctc tcgaatcgat caatgtctac ggcggcacgc aggcgcgtgt gacgaccaac
       61 gacgacgcca tcgccagcta cgccgacgaa atggcccaag gcgccgtctt ccctccgatt
      121 gtcgtttact acgacggcgc aacgcactgg ctcgccgacg gctttcaccg ctacctcgct
      181 gcgaaacgaa acgagcgtcc ctcgattctc gccgaggtcg agcccggcgg tcgcaccgac
      241 gcactgcgcc acgcgctcgg cgccaacgcc acgaacggcg tctaccgcaa caatgccgac
      301 aaacgtaacg cggtcgaaat cgcgctcgaa gaatggccgg atcgcgcgaa tcccgtcatc
      361 gcggaaatct gcaaagtctc cgtcgaactc gttcgtcgca cgcgcaccca actcacgcag
      421 tccggcaaac tctccgcctc cgagaccgtc accgggcgcg acggaaaaac gtatccgtcg
      481 caaatcgaac gccagcctcg cggcaaaacc gaagcttcgt cgagcgatcg cgaatccggc
      541 ggcggcggcg gtttcggcgg caaaggcaag ggcgacggcg gccatctcgg cggcacgtcg
      601 accgagctcg agcgcgacgc ccgcgcgatg attcgcaagg gcgagatcaa tcccttcgag
      661 ctccccaccc tgctcaccgc caacgccgat gattacgccg agaccgtgat caaccttctc
      721 ggcacgatga agcccgacga cccgaggcga agcgccggcc tgctccgcat caagcaatgg
      781 atcgaaaaga cgctcgccgg cgaacccgtc gccgcagccg tcgccgcagc tacgatcgct
      841 tgatccgctc gcattgcgga cggtagcgaa cgcgtgcgcg tttcgtcccc acgtgcgggt
      901 gaaaaacgca tgggtttcac tggattgtga atatttccgt cgagccggtt ccggctcgac
      961 atccgctttg gcatggcacg ccctgtgcac ctccaaagca catgacgaac gtcccctccc
     1021 gctcgacgtc cgcccgcgcc gcacgacgtg gcttttgcgc catcatgctc gccccgtgct
     1081 gcatcgttgc agcggaagac agcgcaccgt ctccctccga cgcgtcgtcg ccgacggttc
     1141 ttcatcccgt cacggtcctc ggctcgcaca tccgtcacac cgatttcgaa ggcccttccg
     1201 ctgtccgcgt ctacgaccgc gactacatcg aggccaccgg cgctacgcgc ctcgcagatt
     1261 ttctcaacct cctcccgcaa aactacggcg gcgccagttc cggccgcggc agcgcgccca
     1321 acgacctcaa cccggagttc ggcgttcgca ccgaaacctc gctgcctttc ttcaacttcg
     1381 ttctcggcat ggccgcgatt ccgttcggcc agaccggcgt ctcggccgcc aatctccacg
     1441 gtctcggcag cggctccacc ctcgtgttgg tcgacggcca ccgccgcccc atcgccggcc
     1501 aaggtaacct ttccacggat acacgccagg gcttcgtcaa tctcaacacg atccctctcg
     1561 ggatgatcga gcgcgtcgag atcctcgccg acggcgcctc cgcgctctac ggttccgacg
     1621 ccatcggtgg cgtcatcaac gtcgtcctca aaaaaaacta cagcggcgcc gagttcgtca
     1681 ccgactacaa agggacgttc gaaggcggcg gtcgcgaacg cagcgtgagc ctcatcgccg
     1741 gcttcacgtc cgacgatgca cgactgcgcg gcacgatcgg catcgatctc tacgatcgcg
     1801 cgccgctcac cgccgcccaa cgccagctct cagcagacca agatcacagc ggtcgtgtca
     1861 tcgaccacga cgccgagggc gatcccattc acggcatcga tttccgtctc aactggagct
     1921 accccgccgt cgtgcaggca cgcaccggca cgctcaacgg cgtcacgcgt cccgacggct
     1981 cgcccgccac cgtcgcgctc gtccccgacg gcgcccgcgc cggactcacc cccgccgatt
     2041 tcattgtctc gccatcgact cgcgccgaaa gcgcgcgccg gatgaacacc gccgcgttca
     2101 tcagcctcat ctctccgcag gaatcgcgca gcgtcagcgg tcaattcgaa taccgcctcg
     2161 gcgaaaatct ccgcgcctac ggcggcgcgt ccgtgggcga agtcgacacg caattcgacg
     2221 gacagcctcc cgtgagcagc gcgtccgctt cgacgggctt cggcagctat cgcaccatcg
     2281 tccccgccat catcgacggt cagccgaaca ccttgaatcc cttcggccag gacgtgctcg
     2341 tcggcctcgt tcatcacgag ttcggaccca cccggcagac cacgcaaacg atcagctacg
     2401 gcgcctacag cgggctgtcc ggacgcttcg ccgactggga atgggacgtc ggcgtcaact
     2461 ggtcgcgcga gcgtttcgaa cagcgcaacc tcaccctcga tctgcagaaa ttcagcgatt
     2521 cgcttgccgc cagcgatcct gcccaacgct tcaacccgtt cgtcgacgcc cgcaccgccg
     2581 gccccgtcaa cgcccacctg tatgaagcga tgaaaaccta cgacgtgcgc cacggccgct
     2641 ccaattacgc cgcgggaagc ttcctcgcgt tcggcccgct gcgcgaattg cccggcggcg
     2701 atcttcaact cgcggtcggt ggcaacctca acaccgccag tcatcgcgcc gagagccgcc
     2761 tcgccaacgg gcccacgaac tccgttcgcg tcgagcgcga cagctttgcc ggtttcgccg
     2821 aagcggtcgt tcccctgttc ggcccgccca atgcccgccc gctcctccga cggctcgaac
     2881 tgcaactcgc cgttcgctac gaacatcatc a
//
LOCUS       DASPLT010000286         3631 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_64638, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000286 DASPLT010000000
VERSION     DASPLT010000286.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 3631)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3631)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3631
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_64638"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..362)
                     /locus_tag="VEA63_03005"
     CDS             complement(<1..362)
                     /locus_tag="VEA63_03005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HYD82984.1"
                     /translation="MKTHLFRLTSLLALCAMVLTAGLRAADTKDKHTFVLVHGATAGG
                     WEWKSTGNFLLADGHTVYRATLTGLGERMHLNGADIDLQTHINDVVNLILFEDLHDIV
                     LTGHSYGGMVITGVMDRVP"
     gene            complement(401..2335)
                     /locus_tag="VEA63_03010"
     CDS             complement(401..2335)
                     /locus_tag="VEA63_03010"
                     /inference="COORDINATES: protein motif:HMM:NF025861.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 97 catalytic
                     domain-containing protein"
                     /protein_id="HYD82985.1"
                     /translation="MKPRWSVPAFFLSLALVLPGQEIKSPNGALAVKLTLDAGALTYA
                     VTHHGRTVFEPSPLGLETSLGSFATALKAAGSVTAKIDEHYTLPHGKVREVHYVANEL
                     TAAFTNANGDKLEVIFRVSDRDVAFAYRLSGKDRRRVTIQREATGFNLPGNATAFATW
                     QAKPGDGWMASKPSYEEGYQIDEPVGTKSPTGLGFTFPALFRIGDTGWVLVSETGTSS
                     NYAGTRLGDPTPHGVYRIAFPEPGENGGIGDATVSASMPLLTPWRTLTVGATLGPIVE
                     STVATDVVQPLYAPSQTYVPGRATWSWLVWQDASMNEADQRTFIDLAAAMGWEYILVD
                     ALWEGNLGRPKLAELVNYARSKKVEVLLWYNSNGAWNDAPQDPRNRMDTAPARQKEMA
                     WLKSIGVKGLKVDFFGGDKQTTLKLYEDILTDGNAHGLMLVFHGATLPRGWERMYPNF
                     MSTEAATVSENLIFSQGFADGEARLSTILSLVRNPVAPLDFGPLVLNRTFHKEAGKGN
                     ARRTTDAFQLATTVLYQSPLQHFGLTPNNLQEQPAFVIDFLKQVPVVWDEARFLSGYP
                     GRHIALARRHGDRWYVVATNGEKQPKELTLDLPFLAGRTLTLIHDGPDQTAATREVTV
                     GADGRLTLSLEVGGGAVLFQ"
     gene            complement(2383..3183)
                     /locus_tag="VEA63_03015"
     CDS             complement(2383..3183)
                     /locus_tag="VEA63_03015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD82986.1"
                     /translation="MKFFRLLAVLTVLGLALPCVSGAGFSLFSRSWGDAIVSTDMTAE
                     GKALAPPTTTQPVYYLGQSLGCKFGSIPGDQLPGEEAMNRFIAKVLAKQGYLGAKAGV
                     HEPSLFLVVQWGYLRPGSGDLLWFLGYDANQDIAAPVFPGLLGPEVWRRGFRSREITT
                     ILDYANGPIYGIIVSAFEYQSASTTKPIIYWQTRIGLPANGKSMAEALPAMVFAAGAA
                     IGREAKSPVLLDVDDARRGYVKLGELDVRGIVGEPEPQGEAGESNQQP"
     gene            complement(3236..>3631)
                     /locus_tag="VEA63_03020"
     CDS             complement(3236..>3631)
                     /locus_tag="VEA63_03020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD82987.1"
                     /translation="VEREYSVRRDPKGRALSGLSMGGRHTMFVGFNSLDLFANFGVLS
                     AGDSETEKTFAAFLNTPDVNAKIDYLFVGLGSIEAQGRFGERSENLRQALVKHGIEHE
                     YYVGGYTAHDWATWRHLLHERFLPNLWRK"
BASE COUNT          677 a         1067 c         1224 g          663 t
ORIGIN      
        1 ggaacgcgat ccatcacacc ggtgataacc atgccgccgt aactgtggcc ggtgagcacg
       61 atgtcgtgga ggtcctcgaa caggatcagg ttcaccacgt cgttgatgtg cgtctgcaga
      121 tcgatatcgg cgccgttgag gtgcatgcgc tcgccgagac ccgtgagcgt ggcgcgataa
      181 acggtgtgac cgtcggcgag gaggaaattg ccggtgctct tccattccca accgccggca
      241 gtggcgccgt ggacgaggac gaaggtgtgc ttgtctttcg tatccgcggc gcggagaccg
      301 gcggtgagga ccatggcgca caaggcgagc aaggacgtga ggcggaacag gtgggttttc
      361 acaagggaag aaagcgaaac cacaatggat gggacggggt tcattgaaac agcacggcgc
      421 cgccgccgac ttcgagtgag agcgtgagcc gaccgtcggc gcccactgtg acctcgcgcg
      481 tggcggcggt ctgatccggg ccatcgtgga taagcgtgag cgtgcggccg gcgagaaacg
      541 ggagatcgag cgtgagttcc ttcggctgct tttcgccgtt tgtggcgacg acataccagc
      601 ggtcgccgtg gcgacgagcc agcgcaatgt ggcggccggg ataaccggag agaaaacgcg
      661 cttcgtccca cacgacgggc acctgtttta ggaaatcgat cacgaacgcc ggctgctcct
      721 gcaggttgtt cggtgtgaga ccaaagtgtt ggagcggcga ttggtagagc acggtggtcg
      781 cgagttggaa ggcgtcggtc gtgcggcgcg cgttcccctt ccctgcttcc ttgtgaaacg
      841 tgcggttcag aacgagcggg ccgaaatcga gcggagcgac gggattgcga acgagcgata
      901 gaatggtgct cagccgcgct tcgccatcgg cgaaaccctg cgagaagatg aggttctccg
      961 agacggtggc ggcctccgtg ctcatgaaat tcggatacat gcgctcccag ccgcggggca
     1021 gcgtcgcgcc gtgaaacacg agcatcagcc catgcgcgtt gccgtcggtg aggatgtctt
     1081 cgtagagctt gagcgtcgtt tgtttgtcgc cgccgaagaa atccactttg aggcctttca
     1141 cgccgatcga tttgagccag gccatttcct tctggcgggc gggcgcggtg tccatgcggt
     1201 tgcgcggatc ctgcggcgcg tcgttccagg cgccgttgga attataccac agcagcacct
     1261 cgactttttt ggaacgcgcg tagttgacga gctcggcgag tttcgggcgg ccgaggttcc
     1321 cttcccagag cgcgtcgacg aggatgtatt cccaacccat cgccgcggcg agatcgatga
     1381 atgtgcgctg atccgcctcg ttcatgctcg cgtcttgcca cacgagccag ctccaggtcg
     1441 cgcggcccgg aacgtaagtt tgcgacggcg cgtagagcgg ttgcaccacg tcggtggcga
     1501 cggtgctttc gacaatcggc ccgagtgtcg cgccgacggt gagcgtgcgc cacggcgtga
     1561 gcagaggcat cgacgcggag accgtggcgt caccgatgcc gccgttttca cccggctcgg
     1621 gaaacgcaat gcggtaaact ccgtgcggcg tcggatcgcc gagacgcgtg cccgcataat
     1681 tgctggacgt gccggtttcg gaaacgagca cccagccggt gtcgccgatg cggaagagcg
     1741 cggggaacgt gaagccgaga cccgtgggcg acttcgtgcc caccggttcg tcgatctgat
     1801 aaccctcttc gtagctcggc ttggaggcca tccagccgtc gccaggcttc gcctgccagg
     1861 tggcgaaggc cgtggcgttg ccgggaagat tgaagccggt ggcctcgcgc tgaatcgtca
     1921 cgcggcgccg gtctttgccc gagagccggt aagcgaaagc gacatcgcga tcgctgacgc
     1981 ggaagatgac ctcgagcttg tcgccgttcg cgttggtgaa ggcggcggtg agttcgttgg
     2041 cgacgtagtg gacctcgcgc accttgccat gcggcagcgt gtagtgctcg tcgatctttg
     2101 ccgtgacgga gccggcggct ttcaacgccg tggcaaagct gccgaggctc gtttcgaggc
     2161 cgagcggcga tggttcgaac acggtgcggc cgtgatgagt gacggcgtaa gtgagcgcgc
     2221 cggcatcgag cgtgagcttc acggcgagag cgccgttcgg gcttttgatt tcctgccccg
     2281 gcagaacgag agccagcgag agaaagaagg cggggactga ccaacgtggt ttcatgagag
     2341 gaaaaagcag aggacgcggc gagcgagcgc ggagtggctg cgtcagggtt gttgattcga
     2401 ttcgccggct tcgccttggg gctccggttc cccgacgatg ccgcggacat cgagttctcc
     2461 gagtttcacg tagccccggc gcgcatcgtc gacgtcgagc agaaccggcg acttggcttc
     2521 gcggccgatc gcggcgccgg cggcgaagac catcgccggc agcgcttcgg ccatggattt
     2581 tccattggcg ggaaggccga tccgcgtctg ccagtagatg atcggtttgg tcgtgctcgc
     2641 cgactggtat tcgaacgcgc tgacaatgat cccgtaaatc gggccattgg catagtccag
     2701 aatggtggtg atctcgcggg aacgaaaacc ccggcgccac acctccggac cgagcagccc
     2761 cggaaagacc ggagccgcga tatcctgatt cgcgtcgtat ccaagaaacc acaacagatc
     2821 accacttcct ggccgcaaat aaccccactg cacgacgaga aacaacgacg gctcatggac
     2881 tcccgccttc gcgccgagat agccctgctt cgcgagcacc ttggcgatga agcggttcat
     2941 cgcctcctcc ccgggaagtt ggtcgcccgg gatggaaccg aatttgcagc cgagggattg
     3001 gccaagataa tacaccggtt gcgtcgttgt gggcggcgcg agcgcctttc cctcggctgt
     3061 catgtcggtc gaaacaatcg cgtcgcccca cgatcgcgaa aacaggctga accccgcgcc
     3121 tgacacgcac ggaagcgcca agccgagcac cgtcaggaca gcgagaagtc ggaagaattt
     3181 catgcgtcgg caaactatga atgcctcgtg tttcgtgccg tcgctcatgc gacggctatt
     3241 tgcgccagag gttcggcagg aagcgctcgt gcagcagatg gcgccaggtc gcccagtcgt
     3301 gcgcagtgta tccgcccaca taatactcgt gctcgatgcc gtgcttcacg agggcctggc
     3361 ggagattctc gctgcgttcg ccgaagcggc cctgcgcctc gatgctgccg aggccgacga
     3421 acagataatc gatcttcgcg ttgacgtcgg gcgtgttgag gaaggcggcg aaggttttct
     3481 ccgtttcgga atcgccggcg ctcaggacgc cgaagttcgc gaacagatcc agcgagttga
     3541 acccgacaaa catcgtgtga cgtccaccca tcgagagacc ggagagcgcg cggcctttcg
     3601 gatcgcgtcg aacggaatac tcgcgctcga c
//
LOCUS       DASPLT010000287         1056 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_43877, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000287 DASPLT010000000
VERSION     DASPLT010000287.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1056)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1056)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1056
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_43877"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..289
                     /locus_tag="VEA63_03025"
     CDS             <1..289
                     /locus_tag="VEA63_03025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD82988.1"
                     /translation="SFGKSMNKLRVDCADGWYELAPFQNYSGVKGTTSDGTPLNATIP
                     NQQAKQMDDDASAILNNTPLLAPGEEGLRDTIVVEAIYRSVAEDGKKVAIR"
     gene            complement(561..893)
                     /locus_tag="VEA63_03030"
     CDS             complement(561..893)
                     /locus_tag="VEA63_03030"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HYD82989.1"
                     /translation="MVSSELLYAEFWSALLAKERSGALSPEQRMQVWELLESHLLDDT
                     IELMRLDAVVVRDVAEIMAIVHPKVPLRMLDAIHLATFAGLEAGPLFSRDKRMAAAAK
                     MLGLVTVE"
BASE COUNT          246 a          335 c          291 g          184 t
ORIGIN      
        1 cagcttcggc aaaagcatga acaagcttcg cgtcgactgc gccgacggct ggtatgagct
       61 cgcgcccttc caaaactaca gtggcgtcaa aggcaccacg agcgacggca ctccgctcaa
      121 cgccacgatt cccaatcagc aggcgaaaca aatggacgac gatgcctccg ctatcctcaa
      181 caacacgccg ctgctcgcgc cgggcgaaga aggcctgaga gacacgatcg ttgtcgaagc
      241 gatctatcgc tcggtcgccg aagatggaaa gaaagtcgct attcgctgat cgggttcgcg
      301 cgacgagcgc aacgcactgc catgatttac gacgaacgag ggtggatacg cgacgctacg
      361 aataacagag atgcttcgcc ttcgctcagc atgacggaga gagagagaga gagagagaga
      421 gagtatataa atgaaagtgc gtctcggaga gacgcaccca ccggcgctac cgcgcggaga
      481 ccgatgcgca tcgctctgcc tttgccgtat tccgcgtggg ttagaacgcg atccgctcaa
      541 gcgtaataaa agctattccg ttactccacc gtcaccagcc cgagcatctt tgctgccgcg
      601 gccatacgct tgtcgcggct gaagagcggg ccggcttcca agccggcgaa ggtcgcgaga
      661 tgaatcgcgt ccagcattcg taacggaact ttcggatgca cgatcgccat gatctccgcg
      721 acatcgcgca cgacgaccgc atccagacgc atcagctcga tcgtgtcatc cagcaggtgg
      781 gattctaaca gctcccaaac ctgcatgcgt tgttccggcg ataatgcgcc ggaacgttcc
      841 ttcgccagca gcgcagacca gaactccgcg taaagaagct ccgacgaaac gatccgatga
      901 tcttagacga gtttctcgca cgccgcggaa tccgcgccga ctccttgtca accgctgccg
      961 tccgctgcgg ccttcatcgt ccttgccccg accacatcag atcacttgtc acccacgccg
     1021 agatcacggc ggtcgaggtc ttccaagcgc cggcgc
//
LOCUS       DASPLT010000288         1302 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_105257, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000288 DASPLT010000000
VERSION     DASPLT010000288.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1302)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1302)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1302
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_105257"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..378
                     /locus_tag="VEA63_03035"
     CDS             <1..378
                     /locus_tag="VEA63_03035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007362655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD82990.1"
                     /translation="TYLTKAGFTPGAGRPSEDIIARAREFAKADGFEWPAFTGRQRMG
                     PGPNPGAVNAPVTRTVYRLVGQGLTARPEAVTVKLGISDGTGTEVIEGLSEGDLVITN
                     VISANPSAPQARPANPFGGGRRF"
     gene            443..1204
                     /locus_tag="VEA63_03040"
     CDS             443..1204
                     /locus_tag="VEA63_03040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HYD82991.1"
                     /translation="MPPVVQLQDIQKTYNSGEVKVHAVRGVSLELHRGEFMAIMGASG
                     SGKSTLMNTLGCLDRPTSGTYLLDGIDVSKLDRNELADLRNEKLGFVFQGFNLLARTT
                     ALENVELPMLYGKHRGSSRELRDRAVHALEIVGLANRADHFPSQLSGGQQQRVAIARA
                     LVNQPQVLLADEPTGNLDSKTSVEVMGVFQKLNDQGITIVMVTHELDIAQYCKRNLIM
                     RDGRVVSDITVTNRLLADAELQKILAAEAEAKLSA"
BASE COUNT          262 a          529 c          301 g          210 t
ORIGIN      
        1 acctatctca ccaaagccgg cttcacgccc ggcgccggcc gtcccagcga agacatcatc
       61 gctcgcgccc gcgagttcgc caaagcggac ggtttcgaat ggcccgcgtt caccggccgc
      121 cagcgcatgg gccccggccc aaatcccggc gccgttaacg cgcctgtcac ccgaaccgtt
      181 taccggctcg tcggccaagg cctcaccgcg cgccccgaag ccgtcaccgt caaactcggc
      241 atctccgacg gcaccggcac cgaagtcatc gaaggcctct ccgaaggcga cctcgtcatc
      301 accaacgtca tctccgcgaa tcccagcgcc ccccaggccc gtcccgccaa ccccttcggc
      361 ggcggccgcc gtttctaacc ataagtccca tccgtcctat ccgtcccata ggtcccaccc
      421 cttcccgctc gcccgccctc ccatgccccc tgtcgtccaa ctccaggaca ttcaaaaaac
      481 ctacaactcc ggcgaagtaa aagtgcacgc cgtccgcggc gtctcgctcg agctgcaccg
      541 cggcgaattc atggcaatca tgggcgccag cggctccggc aagtccaccc tcatgaacac
      601 gctcggttgc ctcgaccggc caacgagcgg cacctacctc ctcgacggca tcgacgtctc
      661 gaagctggac cgcaacgaac tcgccgatct ccgtaacgaa aaactcggtt tcgtcttcca
      721 aggcttcaat ctcctcgctc gcaccaccgc cctcgaaaac gtcgagctcc ccatgctcta
      781 cggcaaacac cgcggttcca gccgcgaact ccgcgaccgc gccgtccacg ccctcgagat
      841 cgtgggcctc gcaaaccgcg ccgatcattt ccccagccag ctctccggcg gacaacaaca
      901 acgcgtcgcc atcgcccgcg cgctcgtcaa ccagccgcag gttctcctcg ccgacgagcc
      961 caccggcaat ctcgacagca aaacctccgt cgaggtcatg ggcgttttcc aaaagctcaa
     1021 cgaccagggc atcacgatcg tgatggtcac ccacgagctc gacatcgcgc agtattgtaa
     1081 acgcaacctc atcatgcgcg acggccgcgt cgtcagcgat atcaccgtca ccaaccgtct
     1141 cctcgccgac gccgaactcc aaaaaatcct cgccgccgaa gccgaagcca aactcagcgc
     1201 ctgattcctc caccacgaat gaacacgaac tcacacgaat acgacgggcg cttcccgctg
     1261 ccctgcctct gtcttcattc gtgtccattc gtgtccattc gt
//
LOCUS       DASPLT010000289         1325 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_3803, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000289 DASPLT010000000
VERSION     DASPLT010000289.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1325)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1325)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1325
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3803"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..342
                     /locus_tag="VEA63_03045"
     CDS             <1..342
                     /locus_tag="VEA63_03045"
                     /inference="COORDINATES: protein motif:HMM:NF024214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HYD82992.1"
                     /translation="IAMLQQFSRSLDGALVTLCDQPAFSAEVIARLIARQRETSRSIV
                     AAHYHGRNAAPALFLREHFPALAALTGEEGARTLLNVRPELVAAENLPELAVDLDTPA
                     DYAAAQSRIDA"
     gene            complement(691..>1325)
                     /locus_tag="VEA63_03050"
     CDS             complement(691..>1325)
                     /locus_tag="VEA63_03050"
                     /inference="COORDINATES: protein motif:HMM:NF014298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HYD82993.1"
                     /translation="RWIDFQRELSVVVARSAAGETKAFPVAENIHTRHILDFSIVPAR
                     VSAAIAREAEVLAVAIAEKLGVVGLIAVEMFLATNGEVLVNELAPRSHNSGHWSIDGC
                     ETSQFEQQVRAVCGAPLGSVDVREPTVMVNILGDAWKWRDGQVVGEPNWSAVLVAPRA
                     KLHLYGKAEPRIGRKMGHFTVRGENLDAVLENARELKTRLHAHESEAAGG"
BASE COUNT          226 a          524 c          341 g          234 t
ORIGIN      
        1 atcgcgatgc tgcaacagtt ctcccgctca ctcgacggcg ctctcgtcac gctctgcgac
       61 cagcccgcgt tttctgccga agtgatcgcg cgcctgatcg cccgccaacg cgaaaccagc
      121 cgcagcatcg tcgccgctca ctaccatggc cgcaacgccg ctcccgcgct tttcctccgc
      181 gaacattttc ccgcgctcgc cgcgctcacc ggagaagaag gcgcccgcac actcctcaac
      241 gtccgccccg aacttgtcgc cgcagaaaat ctccccgaac tcgccgtcga tctcgacacc
      301 ccggccgact acgccgccgc gcaatctcgc atcgacgctt aacccaccac tcgcgcgctc
      361 caattccacc accggcaacc gcaacccagc gcggagggac ggaatgagcc cggctcgtct
      421 tcgccgggaa agtagtgcgg cgcttcagcc cgcagttcgg ctcgcgaggc ggctgaaggc
      481 cgccccactt ctggctcgat agtcgtcgcc taaacggacg attcgaaccg gcagcaacac
      541 gttctctcgg ggcgttttcc cgcgaaggca ccgcagcccg acacggcttt caccgagccg
      601 aacacccggg cttacgccgt gacctccgtg tcatcgctcc gtgacctctg tgttccacac
      661 tcggtcgttt tttgctgcga ttggtccgcc ctaaccgccc gccgcttcgc tctcgtgcgc
      721 gtgcaaccgc gtcttcaact cgcgggcgtt ttccagcacc gcgtccaggt tttctccgcg
      781 caccgtgaaa tgccccatct tgcggccgat tcttggctcc gcttttccgt aaaggtgcag
      841 cttcgccctc ggcgccacga gcaccgcgga ccagttcggc tcgccgacga cctgaccgtc
      901 gcgccatttc cacgcgtcgc cgaggatgtt caccatcacc gtcggctcgc gcacgtcgac
      961 cgacccgagc ggcgcgccgc acaccgcgcg cacctgctgc tcgaactggc tcgtttcgca
     1021 gccgtcgatc gaccagtggc ccgagttgtg cgaacgcggc gcgagttcgt tcaccaacac
     1081 ctcgccattc gtggcgagaa acatttccac cgcgatcaaa cccacgacgc caagtttctc
     1141 ggcgatcgcg accgccagca cctcggcctc ccgcgcgatc gccgcactca cgcgcgccgg
     1201 cacaatcgaa aaatccagga tgtgacgcgt gtgaatattt tccgccacgg gaaacgcttt
     1261 cgtctccccc gccgccgaac gcgccaccac caccgacagc tcgcgttgaa aatcaatcca
     1321 ccgct
//
LOCUS       DASPLT010000290         2596 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_43929, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000290 DASPLT010000000
VERSION     DASPLT010000290.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 2596)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2596)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2596
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_43929"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..541)
                     /locus_tag="VEA63_03055"
     CDS             complement(<1..541)
                     /locus_tag="VEA63_03055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD82994.1"
                     /translation="MISSLFFGRRLCSALVLGAAAASAFFACAATAFAAEPTSDLRQD
                     ALAGLKKAARFYSEKVSSHGGYTYFYSLDLKQAWGEGAATKDQIWTEPPGTPAVGIAF
                     LKAYEATEDLEYLVAATNAGMALVYGQLQSGGWSQTIDFNPKGTHANRYRNGRGAGSN
                     HSSLDDNQTQSSIQFLALLD"
     gene            complement(563..>2596)
                     /locus_tag="VEA63_03060"
     CDS             complement(563..>2596)
                     /locus_tag="VEA63_03060"
                     /inference="COORDINATES: protein motif:HMM:NF027452.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4838 domain-containing protein"
                     /protein_id="HYD82995.1"
                     /translation="FSARIHRLTTLMKINVFSVRRAFIALLGLAGWGRAVSAADAAGF
                     DVVRPGEPIAVIVTADEPSATAKYAVQELVAHVEKATGVKLPVARESAVDASARARIY
                     VGDTSAARAAGIDATALPAETFVLRTKGSALFIAGNDAAHQPLEAAPPFGTFNASAGT
                     LFGVYEWLERDLGVRWVWPGELGTFVPKRNTLSVSAWDETIAPRFFQRNVRPGSFKIE
                     NPALGFTPKARDDYMREQAIFLRRNRMGRSQRLSYGHAYTDWWDKYGEAHPEWFQLVK
                     GKRGPVKPGDRFSMCVSEPGLHRKIVELWLEKGAGTPESLPPSINIVENDYLASCECE
                     RCQAWDGPEPADAMKFYAPNFKVYGTRFTSDRYARFALAVQALAAAHRPDVKVVGYVY
                     FNYFQAPTSGVKLNENIVLGFCPSAGWYPRADDEHEWYKAQWKGWRDTGATLFARTNY
                     FLDGYSMPFIFAHQFAEDFQFQAQHGLAATDFDSLTGQWGTQGPTLYLLMRLHSRPDA
                     PVEHLLAEYYSAFGPAAAAVKAYFDHYENYTMSNRQMIMDTFYDRVALRWRSWAKATH
                     RVFPPESFVAGERLLAEAERAAAGDAEALARVKFLQTGLAHAKLCAAVSASLTTADVE
                     STPERGKVQLEELLRFRRTHEREWFANLTHNAWVEEVSWKLSNETKQEPEYYP"
BASE COUNT          509 a          852 c          752 g          483 t
ORIGIN      
        1 gatccagcag cgcgagaaac tgaatggatg actgggtctg attgtcgtcc agcgacgagt
       61 ggttcgaccc ggcaccgcgg ccattgcggt atcggttcgc gtgcgtgcct ttcggattga
      121 agtcaatcgt ctgagaccat ccgcccgact ggagctgacc atacacgagc gccatgccgg
      181 cgttcgtcgc cgcaaccaag tattcgagat cctcggtcgc ttcatacgct ttcaggaacg
      241 cgattccaac cgcgggcgtg cccggcggct ccgtccagat ctgatccttg gtcgccgcgc
      301 cctcccccca cgcttgcttc agatccaggc tataaaaata ggtgtagccg ccgtgcgaag
      361 acactttctc gctgtagaaa cgcgccgcct ttttcagtcc ggccagtgcg tcctgccgca
      421 gatcgctcgt gggctcagcg gcaaacgcgg tcgccgcgca ggcgaaaaat gccgaggccg
      481 ccgccgctcc caataccaac gccgagcaaa gccggcggcc gaagaagagt gaggaaatca
      541 tggtgtggtt ttcaaaatcg gttcagggat agtattccgg ttcctgtttc gtttcgttac
      601 tcaacttcca actcacttcc tcgacccacg cattgtgggt gagattggcg aaccactcac
      661 gttcgtgcgt gcggcggaat cgaagcagct cttccagctg aaccttgccg cgttccggag
      721 tggactcgac atccgcggtc gtgagcgacg ccgacacggc ggcgcaaagc ttcgcatggg
      781 cgagaccggt ttgcaggaac ttcactcgcg ccagcgcctc ggcatcgccc gcagccgccc
      841 gctccgcctc cgcgagcagt cgctcgcctg cgacaaacga ctccgggggg aaaacccggt
      901 gcgtcgcttt cgcccaactc cgccagcgca aggccacgcg atcgtagaac gtatccatga
      961 tcatctgacg gttgctcatg gtgtaatttt cgtagtgatc gaaatacgcc ttcactgccg
     1021 ccgccgccgg accgaacgca gaataatatt ccgccagcag atgctccacc ggcgcgtccg
     1081 gcctcgagtg cagccgcatc agcagataaa gcgtcggacc ttgcgtgccc cattgtcccg
     1141 tcaacgaatc gaaatccgtc gccgccaagc cgtgctgggc ttggaactgg aagtcttcgg
     1201 caaattgatg agcgaagatg aaaggcatgc tgtagccgtc gaggaagtag ttggtgcgcg
     1261 cgaagagcgt cgctccggtg tcgcgccagc ctttccactg cgctttatac cattcgtgct
     1321 cgtcgtcggc gcgcggatac cagcccgccg aaggacagaa gccgagcacg atattctcgt
     1381 tcagcttcac gcccgatgtc ggcgcctgga agtagttgaa ataaacgtag ccgaccacct
     1441 tcacgtccgg ccggtgcgcc gccgcgagtg cctgcaccgc gagcgcgaag cgcgcataac
     1501 gatcggaggt gaagcgcgtg ccgtagacct tgaaattcgg cgcatagaat ttcatcgcat
     1561 ccgccggctc cggaccatcc cacgcctggc agcgttcgca ttcgcaggag gcgagatagt
     1621 cgttctcaac gatattgatg gacggcggca ggctttccgg ggtgcccgcg cctttctcca
     1681 accagagctc gacgatcttg cgatgcagcc ctggttccga aacgcacatg gaaaaacgat
     1741 cgcccggctt cacgggtccg cgttttccct tcacgagctg aaaccattcg gggtgcgcct
     1801 cgccgtattt atcccaccaa tcggtgtaag cgtgtccgta gctcaagcgc tgactgcgtc
     1861 ccatgcgatt gcgccgcaga aaaatcgcct gctcgcgcat gtaatcgtcc ctggctttcg
     1921 gcgtgaaacc cagtgcgggg ttctcgatct tgaacgaacc gggccggacg ttgcgttgaa
     1981 aaaatcgcgg cgcgatcgtc tcgtcccacg cgctcaccga gagcgtgttc cgcttcggca
     2041 caaacgttcc cagctccccc ggccacaccc agcgcacccc gagatcgcgc tccagccact
     2101 cgtaaacgcc gaagagcgtg cccgccgaag cgttgaacgt gccgaacggc ggcgcagctt
     2161 cgagcggctg atgcgccgcg tcgttgccgg cgatgaacaa ggcgctgccc ttggtcctga
     2221 gcacaaacgt ttccgccggc agcgcggtcg catcgatccc ggctgcccgc gccgcgctcg
     2281 tatcgccgac gtagatacgc gctcgcgcgg aggcgtcgac cgcactctct cgcgcgacgg
     2341 gaagtttaac ccccgtcgcc ttctcgacgt gagcgacgag ttcctgcacc gcatacttcg
     2401 cggtggctga cggctcatcc gcagtcacga taaccgcgat cggctcgcca ggcctcacga
     2461 catcgaagcc cgcggcatcc gcggccgaaa ccgcgcggcc ccaacccgcc agaccaagaa
     2521 gggcgatgaa agcccggcga acgctaaaaa cgtttatttt catcaaagta gtgagacggt
     2581 gaatacgtgc agaaaa
//
LOCUS       DASPLT010000291         1781 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_228294, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000291 DASPLT010000000
VERSION     DASPLT010000291.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1781)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1781)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1781
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_228294"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..401)
                     /locus_tag="VEA63_03065"
     CDS             complement(<1..401)
                     /locus_tag="VEA63_03065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009509771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HYD82996.1"
                     /translation="MRNLARFVAAAGFACHGLIALAQTDTTTKTPSAPTEPIVVLDRF
                     VVGGGEDPTSLMPNQPINVLGLSQKIVETPRSVSVVSGETIERFNITELADLSRFSPS
                     TYTAFSFGVQGGLQIRGDTADTYFGDMKKLN"
     gene            complement(701..>1781)
                     /locus_tag="VEA63_03070"
     CDS             complement(701..>1781)
                     /locus_tag="VEA63_03070"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF012913.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="asparaginase domain-containing protein"
                     /protein_id="HYD82997.1"
                     /translation="VGDGDIPPEELGVDFFSYGPYPASQTRPKLAQSANRVQQALASG
                     DYAGVLWLEGSPNIETSAYWLSLLVDTTLPITLNSAHRNRGAISADGDGNILSSIQYI
                     VSKIWMDENGKNRLGAVMVQDEVIYSAREVVKGDAHPGGYVATGGYGGTLGSMTAGAY
                     VTFVPTRKHTWKSEVRVTALPTNIDGLLRQDGVFKTVPVTIKDERGELIGSAIPKVAI
                     LKGDNWYDDGLPDASAEKGIAATIAMLLEKYPLAGLVGEGLSPYATLSNSQERVLQKA
                     ALSGLPVVKVARGDAHGLVKVNPNNLYIEGNNAIATKARLLLTAALMKYGSLPPAANP
                     ENPTPAELSAIKEKLSLYQAIFDTH"
BASE COUNT          297 a          488 c          630 g          366 t
ORIGIN      
        1 ttgttcagct ttttcatgtc gccgaaatac gtatccgcgg tgtcaccacg gatctgcagg
       61 ccgccttgga cgccgaatga aaatgcggtg taagtgctcg gcgagaagcg cgagagatcg
      121 gcgagctcgg tgatgttgaa acgttcgatg gtttcgccgg agacgacgct gacggagcgc
      181 ggcgtttcga cgattttctg cgacaagccg aggacgttga tcggctggtt gggcatgagg
      241 gaagtgggat cttcgccgcc tccgacgacg aagcggtcga gcacaacaat gggttccgtt
      301 ggggcggacg gcgttttcgt ggtggtgtcg gtttgagcga gggcgataag gccgtgacag
      361 gcaaagcctg ccgcggccac gaaacgcgcg aggtttctca tgagtgtgaa acgtgtaggt
      421 gttaggtgat gtgcgctggc gccgccccgc tgagcaaggc gggtgcccgc gctcggcggc
      481 tccggccttt catccatgaa ttattttcgc gtgaaatccg gtggaacgcg gaaatgacgc
      541 cgcgtatcca tattttgggt tcgcgaggaa aagccgcctc gcgtccggtc gacgagaaat
      601 cgcacggctg aaggtggagc ccgacgtcgt cgcgcgggtt gtttcgcgag cggggcgtta
      661 tcaagcccga cgggacgtcg gactccacca tgacttcgga tcagtgcgta tcgaagatcg
      721 cctggtagag gctgagcttt tccttgatcg cggagagttc ggcgggcgtg gggttttccg
      781 gattggcggc gggcgggagc gagccgtatt tcatcagcgc tgcggtgagg agcaggcggg
      841 cctttgtggc gatggcgttg ttgccctcga tgtagagatt gttcgggttc accttcacga
      901 gaccgtgcgc atcgccgcgc gcgactttga cgacgggcag gccgctgagc gcggcttttt
      961 gcagcacgcg ttcctgggag ttggacaacg tggcgtaggg cgagaggcct tcgccgacga
     1021 gaccggcgag cgggtatttc tcgagcagca tcgcgatggt cgcggcgatg cctttttcgg
     1081 cggaggcgtc gggcagaccg tcgtcatacc aattgtcgcc cttgaggatc gcgaccttcg
     1141 gaatggcgga gccgatcagt tcgccgcgct cgtccttgat cgtgacgggc acggttttga
     1201 aaacgccgtc ctgccgcagg aggccgtcga tgttcgtcgg gagcgcggtg acgcgcactt
     1261 cggatttcca cgtgtgtttg cgcgtcggca cgaaggtgac atacgcgccg gccgtcatcg
     1321 agccgagcgt gccgccgtaa ccgcccgtgg caacgtagcc gccgggatga gcgtcgccct
     1381 tcacgacttc gcgagcggag tagatgacct cgtcctgcac catcacggcg ccgagccgat
     1441 tcttcccgtt ctcgtccatc cagattttgg atacgatgta ttggatcgag ctgagaatgt
     1501 tgccatcgcc gtcggcgctg atggcgccgc ggttgcggtg cgcggagttg agggtgatcg
     1561 gcagcgtggt gtcgacgagc aggctcaacc aatatgcgga cgtttcgatg ttggggctgc
     1621 cctcgagcca gagcacgccg gcgtagtcgc cgctcgcgag cgcctgctgc acgcggttcg
     1681 cggattgcgc gagcttcggg cgcgtttgcg aagcgggata cggaccgtag ctgaagaaat
     1741 ccacgccaag ttcctccggc ggaatatcgc cgtcgccgac g
//
LOCUS       DASPLT010000292         1528 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_85883, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000292 DASPLT010000000
VERSION     DASPLT010000292.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1528)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1528)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1528
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_85883"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1243
                     /locus_tag="VEA63_03075"
     CDS             <1..1243
                     /locus_tag="VEA63_03075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009510957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="BNR-4 repeat-containing protein"
                     /protein_id="HYD82998.1"
                     /translation="KYSGGLGTYTSSHTPMAVYAAAAQRTFFTYGGTSSPDKRELQIM
                     VGSFDHATGRLSQPVLVLDKPEVDDPHDNASLNLDADGHVWLFASGRNTARPGVIFRS
                     REPFSIDRFERVATQTFTYPQPWMIPEKGWLLLFTKYTAGRELYWKTSPDGRTWSEDH
                     KLAGFGGHYQTSGVHEGRKIATFFNYHPDGDVDRRTNIYYAQSTDYGATWTTVDGQPL
                     TLPLASVDNPARVADLESRGELMFTCDLNFDASGHPILLYVASRDGRPGPATAAREWR
                     VTHWDGAAWRTHLVTTSDHNYDMGSLYVEGDTWSIIAPTAPGPQPDSVGGDMVLWTSR
                     DEGRHWTAQQTLTRDSRYNHSYARRPLHARDPFYVFWADGDPRQLSPSHLYFADSTGR
                     HVQQMPITMDTDDVALPVNQR"
BASE COUNT          310 a          529 c          425 g          264 t
ORIGIN      
        1 caaatactcg ggcggactcg gcacctacac gtccagccac acgccgatgg cggtttacgc
       61 cgcggcggcg cagcgcacgt tcttcactta cggcggcacg agttcgcccg acaaacgcga
      121 actccagatc atggtcggct ctttcgacca cgccaccggc cgtctctcgc agcccgtcct
      181 cgtgctcgac aaacccgagg tcgacgatcc gcacgacaac gccagtctca atctcgatgc
      241 cgacggccac gtctggcttt tcgccagcgg ccgcaacacc gcgcgccccg gcgtcatttt
      301 ccgcagccgc gagcctttca gcatcgaccg attcgaacgc gtcgcgacgc agaccttcac
      361 ctatccgcag ccgtggatga tccccgaaaa aggctggctg ctcctcttca cgaaatacac
      421 cgccggccgc gaactctatt ggaaaaccag tcccgatggc cgcacctgga gcgaggatca
      481 caagctcgcg ggtttcggcg gtcattacca gacgagtggc gttcacgagg gccggaagat
      541 cgccacgttc ttcaactacc atccggacgg cgatgtcgat cggcgcacga acatctacta
      601 cgcgcagagc accgattacg gcgccacctg gaccaccgtc gacggccagc cacttacgtt
      661 accgctagcg agcgtggaca atcctgcgcg cgtcgcggat ctcgaaagcc gcggcgaact
      721 gatgttcacc tgcgacctca acttcgacgc gagcggacat cccatcctgc tttacgtggc
      781 gagtcgcgac gggcggcccg gaccagccac cgccgcgcgc gaatggcggg tgacacactg
      841 ggacggcgcg gcgtggcgca cgcatctcgt cacgacgtcc gatcataatt acgacatggg
      901 cagcctttac gtggaaggcg acacctggag catcatcgcc ccaaccgcgc ccggtccgca
      961 gcccgacagc gttggaggcg acatggtgct ctggacgagc cgcgatgaag gtcgccattg
     1021 gaccgcgcag caaacgctga cccgcgacag ccgttacaac cacagctacg cgcgacgccc
     1081 gctccatgcg cgcgatccgt tttatgtttt ctgggccgat ggcgatccgc ggcaattgag
     1141 cccctcgcat ctctacttcg ccgattccac cggccgccac gtgcagcaga tgccgataac
     1201 aatggacacg gacgacgtcg ctctaccggt gaatcaacgt tgaaccgggt gagccaccgg
     1261 gctctacctt ctacggcccg gttgcggctg agttgaaaat cgcacctcgg cttcatcgcg
     1321 ccgattcgcg tatttgaaaa cctcctgagc tctcgttatt ttcccgagaa aggatactcc
     1381 catggcgatc actctcagcg agaacgcagc acgtcgagtg cttgaagtgc gggtgacgga
     1441 aaaattcacg cacgccgact atcagcaatt cgcagcgcgt ttcgaagcga tgctcgagcc
     1501 gcccgcaaaa ctcaacgtgc tcttcgac
//
LOCUS       DASPLT010000293         1010 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_208844, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000293 DASPLT010000000
VERSION     DASPLT010000293.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1010)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1010)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1010
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_208844"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(229..>1010)
                     /locus_tag="VEA63_03080"
     CDS             complement(229..>1010)
                     /locus_tag="VEA63_03080"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HYD82999.1"
                     /translation="ELDAYVTLPAGTSKKKPAPLVVLPHGGPWVRDTWGFDPEVQFLA
                     NRGFAVMQPNYRGSPGYDWKFPDDDLYDFTKMHDDVTDAVKTLLASKLVDPDRVAIMG
                     GSFGAYLALSGVVNEPELYRCAVTIAGVFDWARVMQDSKYDQFTSTRYAFLKRRLGDP
                     KKAEEKFAAISPARRVDQVRVPVFVSHGKEDRVAEVGESKRLISELQKHDVPHEVFLV
                     RGEGHGMARLENRVELYSRVEAFLKKNLAPRETVAAVDSIK"
BASE COUNT          189 a          359 c          267 g          195 t
ORIGIN      
        1 cctgtctcga cgcgatcgcc gacgcgctca tcgcgcggga aaccatttct ggcggcgaag
       61 tccgtcacat cgtggccgag tttcgcgcgt tgtgacaact tggacctcct cgatcctacc
      121 cacgttgccc gacgagcagt cgggcacttc tccccgtcga cctgaccccg catcttgcgc
      181 gcaaggcgcg ctgatgcgcg cgcccgttct gcgcctgact ccaccgcgtt actttatcga
      241 atccactgcc gccaccgtct cgcgcggagc caggttcttt ttcaagaacg cttccacacg
      301 cgaatacagt tccacgcgat tctcgagccg cgccattccg tgaccttcgc cacgcacgag
      361 aaacacctcg tgcggcacgt cgtgcttctg caactcggag atgagccgct tggattcgcc
      421 gacttcggcg acacggtctt ccttgccgtg tgaaacgaac accggcactc gcacctggtc
      481 cacccgacgc gctggcgaga tcgcggcgaa cttttcctcc gctttcttcg ggtcgccgag
      541 ccgccgcttg agaaacgcgt aacgcgtgct ggtgaactga tcgtatttcg aatcctgcat
      601 cactcgcgcc caatcgaaga cccccgcgat cgtcaccgcg caacgataga gctccggctc
      661 gttcacgacg ccagagagcg cgagataggc tccgaacgat ccacccatga tcgccacgcg
      721 atccggatcg accagcttcg aagcgagcag cgtcttcacc gcgtccgtca cgtcatcgtg
      781 catcttcgtg aagtcataga gatcgtcgtc cggaaatttc caatcgtagc ccggtgagcc
      841 gcgataattt ggctgcatca ccgcaaaacc gcgattcgcc aggaactgca cttcgggatc
      901 gaaaccccac gtgtcacgca cccacggacc gccgtgcgga agcacgacga gcggcgccgg
      961 cttcttcttc gacgttcccg cgggcaacgt cacatacgcg tcgagctcac
//
LOCUS       DASPLT010000294         1921 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_25944, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000294 DASPLT010000000
VERSION     DASPLT010000294.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1921)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1921)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1921
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_25944"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1225
                     /locus_tag="VEA63_03085"
     CDS             <1..1225
                     /locus_tag="VEA63_03085"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HYD83000.1"
                     /translation="NTPIIRGTWRNYTLNNDMQTIAADGLLRFETGPVKHQLLIGGQG
                     GVDEFWSPRYYDWDFAKNDYVWRYWSLTDLNPVLEKPDDVSYVFQTKLSTQSRREENT
                     VASAYFTHQGRFLNDRVISLAGVFHSSIDNYIRAFGGEHQRYTAEKTSPQAGVVVKVI
                     EPVSVYALYATSLYPQPGGALGGFGEIFPPVKGKSFEYGVKFDAFESKLSGTVSIYDI
                     RQEGRVIFDATAPNANNPTADPNLPRGANVPTGETRSKGVDVSLYYRLVHDVQLVGSY
                     AYNDTEILHDAQAYLVGRKVTPFFRHRAALWTKYSPSQLKGLSLGVGLTWHGEGIRGY
                     SSSSASGIPNTAKSEYNAQVNLSYTRQIAGYRTTFGVSGRNLLESETVVGGSGDVGYY
                     FPTPREFRFSVMVAF"
     gene            1226..>1921
                     /locus_tag="VEA63_03090"
     CDS             1226..>1921
                     /locus_tag="VEA63_03090"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HYD83001.1"
                     /translation="MKPLIGCVAGFGNIGRSLTRFLRENRAQEVRIIGACDPDPTARN
                     VAAREFGLRATGGFEELLRWKPDFVIIASTSAAHAEQVEQAAAAGCHVFCEKPIALDL
                     VAADRAIAAVERAGVVNQVNYSLRYIEAYRTLHAWKHAGRFGRLLTLTHVRTRGYGLY
                     AGGARHPAVIAPDRSGGWTVHHACHGLDLLYWLNGPFASVYGRVTGTAPAGAEEAVAG
                     MLTFADGAIGHVGD"
BASE COUNT          351 a          562 c          638 g          370 t
ORIGIN      
        1 gaacacgccg atcatccgcg gcacgtggag aaattacacg ttgaacaacg acatgcagac
       61 gatcgcagcc gacgggctgc tgcgcttcga gaccgggccg gtgaagcatc aactcctgat
      121 cggcggccag gggggcgtcg atgagttctg gtcgccgcgt tattacgact gggatttcgc
      181 caagaacgac tacgtgtggc gctactggtc gctgacggac ctcaacccgg ttttggaaaa
      241 accggacgac gtgagttacg tttttcagac gaaactgtcg acgcagtcgc gtcgtgagga
      301 gaacacggtc gcctcggcgt atttcacgca tcaaggccgt ttcctgaacg atcgcgtcat
      361 ctcgctcgcc ggcgtgttcc acagcagcat cgacaactac atccgggcgt tcggaggcga
      421 gcaccagcgc tacacggctg agaagacgag tccgcaggca ggcgtggttg tgaaggtgat
      481 cgaaccggtc tcggtctacg cgctctacgc gacctcgctg tatccacaac cgggtggcgc
      541 gctgggcggc ttcggcgaaa ttttcccgcc ggtgaagggt aagagcttcg agtatggcgt
      601 gaagttcgac gcgttcgaat cgaagctttc cggcaccgtc agcatttacg acatccggca
      661 ggaaggacgg gtcatcttcg atgccacggc gcccaacgcc aacaatccga ctgccgatcc
      721 gaatctgccg cgtggcgcga acgtgccgac gggcgagacg aggtcgaaag gcgtggacgt
      781 cagcttgtat taccgtctgg ttcacgacgt gcagctcgtg ggcagttatg cttacaacga
      841 cacggaaatc ctgcacgacg cgcaggcgta tctggtcggc cgcaaggtca cgccgttctt
      901 tcgccatcgc gccgcgttgt ggacgaaata ttcgccgtcg caactgaaag gcctgtcgct
      961 cggcgtgggc ctcacgtggc acggcgaagg cattcgcggt tactcgtctt cgtcggccag
     1021 tggaattccg aatacggcca aatccgaata caacgcgcag gtgaacctca gctacactcg
     1081 tcagatagcg ggctatcgca cgacgttcgg cgtgagcggc cgcaacttgc tggagtccga
     1141 gacggtggtc ggaggcagcg gcgacgtcgg ttactacttt ccgactccgc gtgaattccg
     1201 attcagcgtg atggtggcgt tctaaatgaa gcctttgatc ggttgcgtcg ccggcttcgg
     1261 caacatcggt cgctcgctga cgcggttcct gagggaaaat cgggcgcaag aggtccggat
     1321 cattggcgca tgtgatccgg atccaacggc gcgaaatgtg gcggcgcggg aattcggtct
     1381 gcgagcgacc ggcggtttcg aggagttgct gcgatggaag ccggattttg tcatcatcgc
     1441 ttcaacgtcc gccgcccatg cggagcaggt cgaacaggcg gcggcggcgg gctgccacgt
     1501 tttttgtgag aagccgatcg cgctcgatct tgtcgcggcc gaccgcgcga tcgccgcggt
     1561 ggagcgggcg ggtgtggtca accaggtcaa ttattcgctg cgttacatcg aggcgtatcg
     1621 gacgctgcac gcgtggaagc acgcggggcg gttcggccgg ctgctcacgc tgactcacgt
     1681 ccgcacgcgc ggctacgggc tgtatgccgg cggcgcgcgt catcccgccg tgatcgcgcc
     1741 agatcggtcc ggcggttgga ccgttcatca cgcgtgtcat ggcctcgatc tgctctactg
     1801 gctgaacggc ccgtttgctt ccgtctacgg ccgcgtgacc ggaacggcgc cagcaggcgc
     1861 ggaggaagcc gtggcgggca tgctgacgtt tgccgacggt gcgatcgggc acgtgggcga
     1921 c
//
LOCUS       DASPLT010000295         2107 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_167610, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000295 DASPLT010000000
VERSION     DASPLT010000295.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 2107)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2107)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2107
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_167610"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(139..591)
                     /locus_tag="VEA63_03095"
     CDS             complement(139..591)
                     /locus_tag="VEA63_03095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007362537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD83002.1"
                     /translation="MKLTQLLRARETLLRQTALANLAFAYQTLDAFAGRIARARLTGS
                     VNLKPADPDADRYWATLTALEGSQSVIEEHFTEEELTDIADAIAYASGRPEFDLTFDL
                     EDFVDRFLIPLRHALEQSGIAVDLPPAHTELPSAHDSQDNSRQTRPSS"
     gene            1132..>2107
                     /locus_tag="VEA63_03100"
     CDS             1132..>2107
                     /locus_tag="VEA63_03100"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HYD83003.1"
                     /translation="MSHPPYDCPRSRHSRALRFRTSAAFGWLALAVFGAAPADAQEGP
                     EGEWWMAAKNYANTRYSALDEITVENARQLQVAWTFSTGLLRGHEAAPLVVGDTMYVV
                     APYPNTLYALDLTKNGAVKWTYEPKPSASAQGVACCDVVNRGAAYWEGKIYYNTLDAH
                     TVAVDAATGREVWKTKLGDINLGETMTMAPIVVKGKVLAGNSGGEFGVRGWIAALDAN
                     TGEIAWRAYNTGPDKDVLIGESFKPFYEQDRGKDLGVTTWPPQAWQMGGGTVWGWISY
                     DAEQDLIFYGTGNPGPWNPEQRPGDNKWTAGIFARDPDTGEARWFYQWT"
BASE COUNT          415 a          546 c          778 g          368 t
ORIGIN      
        1 ggtggacggt agacggtgga cagtgggcgg gcggatcccg ctgctcacgc agcgagccac
       61 tggggacggc agaagggaaa atggaaaggc ccgccgggaa agcggcgggc cggaggttgg
      121 tttttcttct ttgtgaaact acgaacttgg gcgggtttgg cgcgagttgt cctgcgaatc
      181 gtgcgcggag gggagctcgg tgtgagcggg cgggagatcg acggcgattc cggattgctc
      241 gagtgcgtgg cggagcggaa tgaggaagcg gtcgacgaaa tcctcgaggt cgaaggtgag
      301 atcgaactcg gggcggcccg atgcgtaggc gatcgcgtcg gcgatgtcag tgagctcttc
      361 ctcggtgaag tgttcctcga tcacggactg gctgccttcg agggcggtga gggtggccca
      421 gtaacgatcg gcgtcgggat cggcgggttt gagattcacg ctgccggtga ggcgcgcgcg
      481 ggcgatgcgg ccggcaaacg catcgagcgt ctggtaggcg aacgccaggt tggcgagcgc
      541 ggtttgacgc aaaagcgttt cgcgcgcgcg aagaagttga gtgagtttca tgggaagttc
      601 tccttgcatc agcatgcctt ggttgaaggt tgatccgcgc gcaaaggcgc ggggtgttga
      661 gcaaacgcga acggctgacc cacggcgtca aacctaggcg gcagcgcggc tctcgcgtga
      721 cgaaagccgc gctgcgaaca ccaagtctgc tgccactgct aaaatccaat ggagcggcta
      781 cgaatcggac acggctcgat gcgcgaaggc gcccggccga ctgaacctaa cggttgcttc
      841 tgtcggtccg cccggaggtc ggaccctacc tgcctaactt cgaatcacgc tctctctttg
      901 cactctctga tcgacactga ctcgactggc ttgagctgcg aagtgaagag aagcgatgag
      961 catgccgttg tccagcagcg gaacgaaaaa tcttcatcgc ggaagtagga aaaaccggac
     1021 gcgcgcgtag ggagccacgc gtgcgggaac ctttggcggc aaagtgggac acgtgacagc
     1081 gcttcgtctg gcgtcgacac gctgacgaac ggcatccggt tcacgtcgcg tatgtctcac
     1141 cccccttatg attgtccaag gtcgcgccac tcgcgtgcgc tgcgttttcg caccagcgcg
     1201 gcctttggtt ggctcgcgct cgcggtgttc ggagcggcgc cggctgacgc gcaggaaggg
     1261 ccggaaggcg agtggtggat ggcggcgaaa aactacgcga acacgcgcta cagcgcgctc
     1321 gacgaaatca ccgtcgagaa cgcaaggcag ctacaggtcg cgtggacgtt ttcgacggga
     1381 ttgttgcgcg gccacgaagc ggcgccgctc gtggtgggcg acacaatgta tgtcgtcgcg
     1441 ccgtatccaa ataccttata tgcgctcgat ctgacgaaga acggcgcggt gaaatggacc
     1501 tacgagccga agccgtcggc gtcggcgcag ggcgtggcgt gttgcgacgt ggtgaaccgg
     1561 ggcgcggcgt attgggaggg aaaaatctat tacaacacgc tggacgcgca tacggtcgcc
     1621 gtcgatgcgg cgacggggcg cgaggtgtgg aagacgaagc tgggcgacat caatctcggc
     1681 gagacgatga cgatggcgcc gatcgtcgtg aaaggaaagg tgctcgcggg aaacagcggc
     1741 ggggagttcg gtgtgcgcgg ttggatcgcg gcgctggatg cgaatacggg cgagatcgcg
     1801 tggcgggctt acaacaccgg accagacaag gacgtgttga tcggcgagag ttttaaaccg
     1861 ttctacgaac aggaccgcgg caaggatctc ggcgtgacga cgtggccgcc gcaggcgtgg
     1921 cagatgggcg gcggcacggt gtggggctgg atttcctacg atgcggaaca ggatttgatt
     1981 ttttatggga cgggaaatcc gggaccgtgg aatccggagc agcggccggg agacaacaag
     2041 tggacggcgg ggatcttcgc gcgcgatccg gatacgggcg aggcgaggtg gttctatcag
     2101 tggacgc
//
LOCUS       DASPLT010000296         6477 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_228342, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000296 DASPLT010000000
VERSION     DASPLT010000296.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 6477)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6477)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6477
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_228342"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2911
                     /locus_tag="VEA63_03105"
     CDS             <1..2911
                     /locus_tag="VEA63_03105"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HYD83004.1"
                     /translation="AGFLCPGLNAQTAPTSTTAAEEDVVELSPFTVTSDKDKGYRATN
                     SVAGTRLATAIKDVPMSIEVITSDFIRDTGSTSLRESLRFSGGIVLSSQVDGLVDPGS
                     LDTKANAGANDPRGVDRDPNGTTLKIRGFVIDQVLRDGFRRQSSSDSINIDRIEVVRG
                     PSALLYGVGNFGGVVNYLPKEPKLKPAYSLEFGAGNWGYKRGALDFTGPLGDPDNRWE
                     AAYRLTAAVQERGDGTQFFNQKSWLVSPVFSFRPFPNTYVKIDVEVGATNMNGMGFQN
                     VRSRVGGSGAQQRISNFVTSVLGSGLDARTFRWSGPDTYTNAPMRNFALTVEQKITDD
                     LIVRVGAQTGRTERESLNIFAGTIIAGAGTFNGTRPTDAMRLALLGSPVPLPISLAGE
                     DQNLNRDGQATSTANAILRYQWSQYFENEDRDQIRAEVAYGFKLLGDHKLLAGYQYQG
                     RNRESWTFAPRGSGDNRDRFNFKNPMDFTHIRFGKQGDGMPDVELVRDRWDKGVTWDS
                     GFYGVYQGRFWKDRITLIGGVRHDRSDQRNYSRNYQGNGALVVTDRSAARPDAPSATS
                     PQYGVSFAVTREVSLYALHSEGLVPQYFIRDGLGRPFDPTKAENNEVGAKFDLFNGRV
                     SGTVGVFKIEREGTPRNIWWAPSATNGVPYDPALPRTYRVGGNPSSPQFIATYRNEPW
                     WQQVVGNRDLATQQVLVNVDTEAGKQFMRSAFNWAENHPFDFPAAFMFIGNGTASVFG
                     DGTPGVVNTPGVTSNANVQINDEATGWDTQWIISPTDNWQIVATYANTRTKVTSGYNY
                     IKAPIDDEFAMWKFPWIGWGTVVGIPASEAFEDPTDSSTFKGIGTGLGETLDDTPKHT
                     VTLWTKYIFTNNTLKGWSIAGGAHYMSERPYQTGFSIDGTGIVVLGDDGRPKPLKLYT
                     DEQYSFSGMVEYRTNFGKYETRFALNVDNLLNDKKLYGYLYAPGRSYRFSVSTNF"
     gene            3120..4979
                     /locus_tag="VEA63_03110"
     CDS             3120..4979
                     /locus_tag="VEA63_03110"
                     /inference="COORDINATES: protein motif:HMM:NF019552.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heparinase II/III family protein"
                     /protein_id="HYD83005.1"
                     /translation="MTALFVGGLFALGGLRAAEKAPSLETGLPSHPRLFANAARWEAL
                     RDQTKTDSVSALLAEALFLRAEEALALPPIRYEKEGRRLLRPVREGVSRILVLGMAAR
                     LGDDVRFRERAIDEMRAVAALPDWNPSHFLDTAEMTFGLAIGYDWLFEWLSAEDRVRI
                     ADAIVEKGLKASFPSDESEPGWAEARNNWTQVCNSGLVAGAIAVAPRESELGRKVLER
                     AFASLPGAAELAYAPDGAYPEGPSYWAYGTIYHAILVDVLEHALGSAFGLDAFPGFLE
                     SSQYVNQMTTPRGEYFNYGDNEERRGFGPAMFWFARKLHDRSIIARDLAAIEPMIERM
                     RAGTAEGNLRFSPLALLWWDPSLDREANDAKREAPLSWMGRGLMPVAVHRTAWDDRNA
                     GFVGIKGGAIQASHAHMDLGSFIVELGGVRWAVDLGPEDYHPLEQRGLNLWSYHQGAD
                     RWKVFRLGSEGHNILRFDGADQDVWAKAPIVGFGDAADNRYTTVDISSVYRPFVDSAR
                     RTVRLENAARVTIEDEWLTGDEATEVAWQWLTRANVEVNREGATLRQEGRTLRLRVVE
                     PASGWTLAVEETSKLMRAHDTPDPELRRIVLHTKTAAHAEGRIVVVAELEDGD"
     gene            complement(5162..5839)
                     /locus_tag="VEA63_03115"
     CDS             complement(5162..5839)
                     /locus_tag="VEA63_03115"
                     /inference="COORDINATES: protein motif:HMM:NF024863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HYD83006.1"
                     /translation="MPPIVPHGFALLQSPSFRAPHFGSQLRRILTLGVILGATFMSSA
                     NENPTTAPAPSIAMIGDSITRQGDWTRLLGREDVINWGHPGYTTGQLAWTFKDLIRQH
                     PNIKIVFLSGGTNDLLLGVPVARIFENQVAAIRYWRERGVTPVLQSLLPQVDAPETNA
                     LIKSLNAQLRAHCTAENVRFLDLTPLFAVGDALRPELSADGTHLKPPAYAPWAEQVSQ
                     ELKALGH"
     gene            6122..>6477
                     /locus_tag="VEA63_03120"
     CDS             6122..>6477
                     /locus_tag="VEA63_03120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosidase"
                     /protein_id="HYD83007.1"
                     /translation="MTLVGDSIPNLPWQPKPSGCLDIVWRYDANPIVGRRPLPQATGI
                     YNSAVVPFGGGFAGVFRTEYKDRMPHLHTGWSDDGIRWRFDADEIRFRCDDAAVAKWE
                     YGYDPRVTRIGDDYYVT"
BASE COUNT         1224 a         1820 c         2180 g         1253 t
ORIGIN      
        1 cgccggcttt ctttgccccg gcttgaacgc gcagacggcg ccgacaagca ccacggccgc
       61 ggaagaggac gtggtcgaac tatccccgtt caccgtcacg agcgacaagg acaagggtta
      121 ccgcgcgacg aactcggtcg ccggcacgcg cctggcaacg gcgatcaagg acgtgccgat
      181 gtcgatcgag gtgatcacca gcgacttcat ccgcgacacc ggctcgacga gcctgcgtga
      241 gtcgctgcgc ttctcgggcg gcatcgtgtt gagttcgcaa gtggacggtc tcgtcgatcc
      301 gggaagtctc gataccaagg cgaatgcagg cgcgaacgat ccgcgcggtg tggatcgtga
      361 tccgaacggc acgacgctga agattcgcgg cttcgtgatc gaccaggtgt tgcgcgacgg
      421 cttccgccgg cagtcgagtt ccgactcgat caacatcgac cgtatcgaag tggtgcgcgg
      481 tccctcggcg ttgctctacg gcgtcggcaa cttcggcggc gtcgtgaact acctgcccaa
      541 ggagccgaag ctgaagcccg cgtattcgct cgagttcggc gcgggcaact ggggttataa
      601 gcgtggcgcg ctcgatttca ccggtccgct cggcgacccc gacaatcgct gggaagcggc
      661 ctaccggctg acggctgcgg tgcaagagcg cggtgacggc acgcagttct tcaatcaaaa
      721 atcctggctc gtttcaccgg tgttcagttt ccggcctttc ccgaacacgt atgttaaaat
      781 cgacgtcgaa gtcggcgcga ccaacatgaa cggcatgggc ttccagaacg tgcgctcgcg
      841 cgtgggcggc tcgggtgcgc aacagcgcat ctccaatttc gtgacgagcg ttctcggctc
      901 gggtctcgat gcccgcacct tccggtggtc gggtccggac acctacacaa atgcgccgat
      961 gcggaatttc gcgctgacgg tcgaacagaa gatcacggac gatttgatcg tgcgggtggg
     1021 tgcgcaaacc ggccggacgg aacgcgagag cctcaatatt ttcgccggca cgatcatcgc
     1081 tggcgcggga acgttcaacg gcacccggcc gaccgacgcg atgcggctgg ccttgctcgg
     1141 ctcgcccgtg ccgctgccga tctcgctggc cggcgaggat cagaatctca atcgcgacgg
     1201 gcaggccacc tccacggcta acgcgattct gcggtatcag tggtcccaat acttcgaaaa
     1261 cgaggaccgc gaccagatcc gcgcggaagt cgcctacggc ttcaaactgc tcggcgatca
     1321 caagctgctc gccggttacc aataccaggg gcgcaatcgc gaatcgtgga cctttgcgcc
     1381 gcggggctcg ggcgacaatc gcgaccgttt caatttcaag aacccgatgg atttcaccca
     1441 tatccgtttt ggcaagcagg gcgacggcat gcccgacgta gagctggtgc gggatcgctg
     1501 ggacaagggt gtcacgtggg attccggctt ctacggcgtt taccaaggcc ggttctggaa
     1561 agatcgcatc acgttgatcg gcggcgtccg tcacgatcgt tcggatcaac gcaactactc
     1621 gcggaactac cagggtaatg gcgcgctcgt ggtcaccgat cgttctgcgg cgcgcccgga
     1681 tgcgccgtcg gcgacgagcc cgcaatacgg cgtgagtttc gccgtcacgc gcgaagtgtc
     1741 gctctacgcg ctgcattccg agggcctggt cccgcaatat ttcattcgcg acggtctcgg
     1801 tcgtccgttc gatccgacaa aggcggagaa caacgaagtc ggcgcgaagt tcgacctgtt
     1861 caacggacgc gtttccggaa cggtcggcgt cttcaagatc gaacgcgaag gcacgccgcg
     1921 caatatctgg tgggcgcctt cggcgaccaa tggcgtgccg tatgatcccg cgctgccccg
     1981 cacctaccgc gtcggcggca atcccagctc gcctcagttt attgccacgt atcgaaacga
     2041 gccgtggtgg cagcaggtgg tgggcaaccg cgacctcgcc actcagcagg tgctggtgaa
     2101 cgtcgatacg gaagcaggaa aacaattcat gcgatcggcg ttcaattggg cggaaaacca
     2161 tcccttcgat tttcccgcgg ccttcatgtt catcggcaac ggcacggcga gcgtgtttgg
     2221 tgacggaacg cccggcgtcg tcaacacccc cggtgtcacc tccaacgcga acgtgcagat
     2281 caatgacgag gcgaccggtt gggatacgca atggatcatc agccccacgg acaattggca
     2341 gatcgtcgcc acctacgcga acacgcgcac gaaggtgacg tccggctaca actacatcaa
     2401 ggcgccgatc gacgacgagt tcgcgatgtg gaagttcccg tggatcggct ggggcaccgt
     2461 cgtcggcatt ccggcgagcg aagcgttcga ggatccgacc gattcgtcga ctttcaaggg
     2521 catcggcacg ggcttgggcg agaccctcga tgacacgccg aagcatacgg tgacgctctg
     2581 gaccaagtat atattcacga acaacaccct caagggttgg tcgatcgccg gcggcgctca
     2641 ctatatgtcg gagcggccct atcagactgg tttctcgatc gacggcaccg gcatcgtggt
     2701 gctcggcgat gatgggcgac cgaagccgct gaagttgtat acggacgagc aatacagctt
     2761 ctctgggatg gtcgaatacc ggacgaactt cggcaaatac gaaacacgtt tcgcgctgaa
     2821 cgtggataat ctgctcaacg ataaaaaact ctacggctat ctttacgcgc cgggccgtag
     2881 ctatcgcttc agcgtcagca ccaacttcta agcggatagg ttgtgcgctg gcgcacaacg
     2941 gggcgcggcg agcgcgcccc ctacaagact gaattgagtt cgatgcaagc ggcgggacgg
     3001 cggttgtccc gccgcttttt catgaaggaa cgatcgcaat gattccgaag tttatgaacc
     3061 catcttcctc tgcggcacat gcgagtggaa tcaggctgaa cgtgctgcgc cgtggatgcg
     3121 tgactgcgct gttcgtcggc gggctgttcg cgctgggtgg tttgcgggcg gccgaaaaag
     3181 cgccgtcgct cgaaacgggt ttgccgtcgc atccgcggct cttcgcgaat gccgcgcgtt
     3241 gggaggcgct ccgcgatcag acgaaaacgg attcggtgtc ggcgctttta gcggaggcgt
     3301 tgttcctgcg cgcggaggag gcgctcgcgc tgccgccgat tcgctacgaa aaagaaggcc
     3361 ggcggctgct tcgccccgtg cgcgaaggcg tgtcgcgtat ccttgtgttg ggcatggcag
     3421 cgaggttggg agacgacgtc cggtttcgcg aacgcgcgat cgacgagatg cgcgcggtgg
     3481 cggcgttgcc cgactggaac ccgtcacatt ttctggatac ggcggagatg accttcggac
     3541 tcgcgatcgg ctacgattgg ttgttcgagt ggctgtccgc cgaagatcgc gtgcgcatcg
     3601 cggatgcgat cgtggagaaa ggattgaagg cttcgtttcc gagcgatgag agcgagccgg
     3661 gctgggcgga ggcgcggaac aactggacgc aggtgtgcaa cagcggcttg gtcgcgggcg
     3721 cgatcgcggt cgccccgcgt gagtccgagc tcgggcgaaa ggtattggag cgcgcgttcg
     3781 cttcgttgcc gggcgcggcg gagctcgcgt atgcgccgga cggagcgtat ccggaaggtc
     3841 cgtcgtattg ggcctacggc acaatttatc atgcgatcct ggtcgacgtg cttgagcatg
     3901 cgttgggatc cgcctttggg ctggacgcgt ttcccgggtt tctcgaatcg tcgcagtatg
     3961 taaaccaaat gaccacgccg cgcggcgagt atttcaacta cggcgacaac gaggagcggc
     4021 ggggttttgg gccggcgatg ttttggttcg cgcggaagtt gcacgatcga tcgatcatcg
     4081 cgcgcgacct cgcggcgatc gagccgatga tcgaacggat gcgcgcggga acggccgaag
     4141 ggaatctgcg cttttctccg ctcgcgctgc tctggtggga tccgtccttg gatcgcgagg
     4201 cgaacgacgc gaagagggag gcgccactga gctggatggg gcgcggcttg atgccggtgg
     4261 cggttcatcg cacggcgtgg gatgatcgaa atgcgggctt tgtcggaatc aaaggcggcg
     4321 cgatccaggc ttcgcatgcg cacatggacc tcgggtcatt tattgtggag ttgggtggcg
     4381 tgcgctgggc ggtcgatctg gggccggaag attatcatcc gctcgagcag cgcggtttga
     4441 atctgtggtc gtatcatcaa ggcgcggatc gctggaaagt ttttcgcctg ggatcagagg
     4501 gccacaatat cctgcgtttt gacggagcgg atcaggatgt gtgggccaaa gcaccgatcg
     4561 tcggattcgg cgatgcggcg gacaatcgtt acacgacggt cgatatcagc tcggtttacc
     4621 gaccgtttgt cgacagcgcg cgccgcacgg tgcggcttga gaatgcggcg cgcgtgacga
     4681 tcgaggacga gtggttgacc ggcgatgagg cgacggaggt ggcttggcag tggctcacgc
     4741 gcgcgaacgt cgaagtgaac cgcgaaggcg cgacgctgcg acaggagggg aggacgctgc
     4801 gcttgcgcgt cgtcgagcct gcctcgggct ggacgctcgc ggtggaggag acgagtaaac
     4861 ttatgcgcgc tcatgacacg ccggatccgg aactgcgccg aatcgtgctg cacacgaaaa
     4921 cggcggcgca cgcggagggg aggattgtgg tcgtcgcgga gttggaggat ggggattgag
     4981 ccggcgcggc gcgatgaggc aggccgcgcc gctgaagttg tagggcgggg tctcctgacc
     5041 ctgccgtgtt gggcgttcgc gaaacgttgt tcgcggcggg gtcgggagac cccgccctac
     5101 aacagcagtg cagatcgcgt gaatgatctg cgacggattc tcgtgggcga tctgcgtggg
     5161 ctcagtggcc gagcgctttt agctcttggc tcacctgttc agcccaaggc gcgtaggcgg
     5221 gcggtttcaa atgcgtgccg tcggcgctca gctccgggcg cagcgcgtcg cccacggcga
     5281 aaagcggcgt gagatcgagg aagcgcacgt tctccgcggt gcaatgcgcg cggagctgcg
     5341 cgttgagcga tttgatgagc gcgttcgtct cgggggcgtc gacttgagga aggagcgatt
     5401 gcagcacggg cgtcacgccg cgttcgcgcc aatagcgaat ggcggcgacc tggttctcaa
     5461 aaatacgcgc gacgggaacg ccgaggagga ggtcgttggt gccgccggag aggaaaacga
     5521 ttttaatgtt tgggtgctgc cggatcaggt ctttgaacgt ccacgcgagc tggccggtgg
     5581 tgtagccggg atgaccccaa ttgatcacgt cctcgcgacc cagtagtcgg gtccaatcgc
     5641 cctggcgtgt gatcgagtcg ccgatcattg cgatcgatgg ggcgggcgcg gtggtggggt
     5701 tctcgttggc ggagctcatg aaggtggcgc cgagaatcac gccgagggtg aggattcggc
     5761 ggagttgaga gccgaagtga ggcgcgcgaa atgagggaga ttgcaggagg gcgaatccgt
     5821 gggggacgat gggtggcatg gccggcttaa ggggaatctg gcgctttttg tgggttagtc
     5881 atgtttacgt gacaacgtaa atagatgcat gcactcacgt tgatatcctt ccaacatatg
     5941 atggttaaat agataatgat taaatgataa cagtttaaac acctgtcact tatcgtaaag
     6001 ttatcagcga cggcaaaact cgaagaaaat tgttgccata tgataaatcg cgccctgcat
     6061 tcacgtcaga ccctacggcg ccgacccaaa cttctacggc gctttcaacc ttctgagttc
     6121 catgaccctt gtcggcgatt ccatccccaa tttgccttgg caacccaagc cgtccggctg
     6181 tctcgatatc gtgtggcgat atgatgccaa tccgatcgta ggccgccgtc cgcttcccca
     6241 agcgacgggg atttacaaca gtgcggtggt gccgttcggc ggcgggttcg cgggtgtgtt
     6301 tcgcacggaa tacaaggatc ggatgccgca tcttcacacg ggttggagcg atgacggcat
     6361 tcggtggcgg ttcgacgcgg acgagattcg cttccgttgc gacgacgccg cggtggcaaa
     6421 gtgggaatat ggatacgatc cgcgggtgac gcgcatcggc gacgactatt acgtgac
//
LOCUS       DASPLT010000297         1384 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_3933, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000297 DASPLT010000000
VERSION     DASPLT010000297.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1384)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1384)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1384
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3933"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..334)
                     /locus_tag="VEA63_03125"
     CDS             complement(<1..334)
                     /locus_tag="VEA63_03125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HYD83008.1"
                     /translation="MASIKHVFSEIHYEMVRKIADGGMGVVYEAEQLGSGRFRKVVAV
                     KLIREEFSAVEEFQNNFIGEARLVADLIHTNIVQTYHLGQVGGQYFMVMEFVRGVNLE
                     QFLDKHREL"
     gene            460..777
                     /gene="erpA"
                     /locus_tag="VEA63_03130"
     CDS             460..777
                     /gene="erpA"
                     /locus_tag="VEA63_03130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007363931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HYD83009.1"
                     /translation="MITLSPRAAQRVRSMHAELNEPAKRLRVFVESGGCSGFQYGMSF
                     DEPKPDDAQLESEGVSMLLDPASLAYLTGSVIDFDDGLHGKGFEIKNPNATSTCGCGK
                     SFS"
     gene            complement(964..>1384)
                     /locus_tag="VEA63_03135"
     CDS             complement(964..>1384)
                     /locus_tag="VEA63_03135"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009508477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HYD83010.1"
                     /translation="TANITFRQGDGLALPLADASFDVVTISFGLRNMADRHRALTEMR
                     RVLRPGGRLFVLEFSQPDAWFRPFYLFYLRRILPVAAGLLTGDRAAYRYLNETIEQFP
                     GREALAAEIRGAGFPTVFARPMTFGIVALHEALRSDA"
BASE COUNT          291 a          383 c          456 g          254 t
ORIGIN      
        1 caagttcgcg gtgcttgtcg aggaactgct cgaggttcac gccgcggacg aactccatca
       61 ccatgaaata ctgaccgccg acctgcccga gatggtaagt ttggacgatg ttggtgtgga
      121 tcaaatcggc gacgagccgc gcttcgccga tgaaattgtt ctgaaattct tcgaccgcgg
      181 agaactcctc gcgaatgagt tttacggcga cgacctttcg aaaacgcccc gagccgagct
      241 gctccgcttc atagacgacg cccatgccgc cgtcggcaat cttgcggacc atttcgtaat
      301 gaatctcgct gaaaacgtgt ttgatggacg ccacgacggc gccgagagaa caggccaccg
      361 caagcactgg caagtgctca atggcaattc ggcgcgaaaa ttgctgtagg agcgcggcga
      421 ggcgtttgac agggtcgcga gcgatcctaa tttcgcggca tgatcacgct ttcaccacgc
      481 gctgcgcaac gtgttcgttc gatgcacgcc gagctcaatg agccggcgaa gcggctgcgg
      541 gtgttcgtcg aatcgggcgg ctgctcgggt ttccaatatg gcatgtcgtt cgacgagccg
      601 aagcccgatg acgcgcagct ggagagcgaa ggcgtgtcga tgctgctcga tccggcgagc
      661 ctggcttatt tgacgggctc ggtgatcgac ttcgacgacg ggctgcacgg caagggcttc
      721 gagattaaaa atcccaatgc caccagcacg tgtggttgcg ggaagtcgtt tagctaagcg
      781 cttggctgaa gcgtgaggtg aggcttggaa tagaatcgta gcaggatcgt agccgcgcgc
      841 gggtgggtct ctgagccgca ctcatgcagt agaaggtgaa gcccgacgtc ctcgccggcc
      901 ttttttgcga gcgacacctg aacgagcccc gacgggacgt cgggctccac ctttcgatcc
      961 aggttacgcg tccgagcgca gcgcttcgtg gagggcgacg ataccgaagg tcatgggccg
     1021 ggcgaagacg gtgggaaaac cggcgccgcg aatctcagcg gcgagagcct cgcgtccggg
     1081 gaactgctcg atcgtttcgt tgagataacg atacgcggcg cgatcgccgg tgagcaatcc
     1141 ggcggcgacc ggaagtattc gacggagata aaaaagataa aacgggcgaa accacgcgtc
     1201 cggttgcgaa aattccagca cgaagagccg gccgccgggg cgcagcacgc gtcgcatttc
     1261 agtgagcgcg cggtgacgat ctgccatgtt gcgcagccca aatgagatcg tgaccacgtc
     1321 gaagctggca tcggcgagcg ggagggcgag cccgtcaccc tggcgaaagg tgatgttggc
     1381 ggtg
//
LOCUS       DASPLT010000298         1224 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_208875, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000298 DASPLT010000000
VERSION     DASPLT010000298.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1224)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1224)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1224
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_208875"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..332
                     /locus_tag="VEA63_03140"
     CDS             <1..332
                     /locus_tag="VEA63_03140"
                     /inference="COORDINATES: protein motif:HMM:NF018168.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="flagellar basal body rod C-terminal
                     domain-containing protein"
                     /protein_id="HYD83011.1"
                     /translation="DVALAISQLATKKFSTTGAPPDVIDGTFGSFFSGAVSGLGQAVS
                     GATSRVNDQSRIEQLVRAQRDAVSGVSLDEEMADLMKYQRAFQASSRVFTTIDDLLEV
                     VVNQLGR"
     gene            441..>1224
                     /locus_tag="VEA63_03145"
     CDS             441..>1224
                     /locus_tag="VEA63_03145"
                     /inference="COORDINATES: protein motif:HMM:NF012874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HYD83012.1"
                     /translation="MRIASNTMSDVMIRQIQQLTGEQAKLQLQVSTGRRITQPEDDPT
                     AVGRVLTLQSEKRQIGQYASNAIRSLTLAQTSFSGLQGLKKVSDRAGELATLGTGAMS
                     SDAMNAYATEVDQMLEQALQLANSRAGSDYIFAGTAVDAPAFSATRDANNKITTLTFD
                     GNTSQAVIPLSEATSVSPSTSGTTNQGIGDFLNHLVALRDALTAGDIDAVRAVQPGLT
                     DDEDMLIAAMADNGGIQTRIEAAQVQQQDRATNIEKLISNETS"
BASE COUNT          255 a          421 c          334 g          214 t
ORIGIN      
        1 acgatgtcgc gctggccatt tcgcagctcg cgacgaaaaa gttctccacg accggcgcgc
       61 caccggatgt gatcgacggc accttcggtt cgtttttctc cggcgcggtc agcggactcg
      121 gccaggccgt ttccggcgcc acctcccgcg tcaacgacca gtcgcgtatc gagcagctcg
      181 tgcgcgccca gcgtgatgct gtcagcggcg tgtcgctcga cgaagagatg gccgacctca
      241 tgaaatacca acgcgcgttt caggcatcgt cccgcgtctt caccaccatc gacgacctcc
      301 tcgaagtcgt cgtcaaccag ctcggtcgct aactggccga agtatgaagt atcaagtaac
      361 aagttccaag atggcgtccg cgcgcaccgc gtatcttgat acttgatact tgttatctgg
      421 aacttcccac cttcaccttc atgagaatcg cctccaatac gatgtcggac gtaatgatcc
      481 ggcagatcca gcagctcacc ggagagcagg cgaagctgca gttgcaggtc tcgaccggcc
      541 gtcgcatcac gcagcctgag gacgatccca ccgccgtcgg tcgcgtgctt acgctccaga
      601 gcgaaaagcg tcagatcggc caatacgcga gcaacgccat ccgctcgctc acgcttgcgc
      661 agacttcgtt ttccggcctc caaggcctca agaaagtttc cgaccgcgcc ggcgagctcg
      721 ccacgctcgg caccggcgcc atgagctccg acgcgatgaa cgcttacgcg accgaggtcg
      781 accagatgct cgagcaagcg cttcaactcg cgaacagccg cgccggcagc gactacattt
      841 tcgcgggcac cgcggtcgac gctccggcct tttcggccac gcgcgacgcg aacaacaaga
      901 tcacgacgct caccttcgac gggaacacca gccaggcggt gattccgctc tccgaggcga
      961 cgagcgtgtc gccttccacc tcgggcacga ccaatcaggg catcggagat tttctgaatc
     1021 atctcgtcgc gcttcgcgat gcgttgaccg cgggcgacat tgacgccgtc cgcgccgtgc
     1081 aacctggcct caccgatgac gaagacatgt tgatcgcagc catggcagac aacggcggca
     1141 ttcaaacccg catcgaggcc gcgcaagtcc agcaacaaga tcgcgcgacc aacatcgaaa
     1201 aactcatctc gaacgagacc tccg
//
LOCUS       DASPLT010000299         1894 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_3975, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000299 DASPLT010000000
VERSION     DASPLT010000299.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1894)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1894)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1894
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3975"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..244
                     /locus_tag="VEA63_03150"
     CDS             <1..244
                     /locus_tag="VEA63_03150"
                     /inference="COORDINATES: protein motif:HMM:NF018178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="family 16 glycoside hydrolase"
                     /protein_id="HYD83013.1"
                     /translation="DFRAPRFDAEGRKTGNARLTAWLNGILIHDNIELPHPTGGSPAG
                     GREQKDVPGPQGFMLQNHGGLLQFRHIWVASETAPR"
     gene            400..>1894
                     /locus_tag="VEA63_03155"
     CDS             400..>1894
                     /locus_tag="VEA63_03155"
                     /inference="COORDINATES: protein motif:HMM:NF024240.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HYD83014.1"
                     /translation="MPRTCLLLLVTALFSTLAVAARDYDVVIYGGTSAAVTAAVQAKK
                     MGKSVIIVGPDKHLGGLSSGGLGWTDTGNKTIIGGLSREFYQRVWQHYNRPDAWTWQD
                     ADDYRTGRRTRTAIDDATKTAWNFEPHVAEKVFEDFIREHDIPVHRDEWLDRAKGVKL
                     QDGRIVSITMLSGQTYAGKMFIDATYEGDLMAAAGVSYHVGREANRVYGEEWNGVQVG
                     ILHHRHHFGPYKAASSLPAGHALEISPYVVPGDPSSGVIARVSTEPPGIRGEGDHRVQ
                     AYCFRMCLTDVPENRVPFAKPAGYDPAQYELLARIYAAGWKETFGKFDPLPNHKTDTN
                     NHGPMSTDNIGYNYDYPDASYERRKEIVQEHATYQQGWLYFIANDPRVPAEVRNEMQR
                     WGLPKDEFKDNGHWSHQLYVREARRMIGAYVMTENELRKKRPTPDSVGMGSYTIDSHN
                     VQRYITPEGHVQNEGDIGVSTGGPYAIAYGALVPKREQASNLLVPVCL"
BASE COUNT          382 a          684 c          501 g          327 t
ORIGIN      
        1 cgacttccgc gctccccgtt tcgacgccga gggccgcaaa accgggaacg cacgcctcac
       61 cgcctggctg aacggcattc tgattcacga caacatcgag ctgcctcatc ccacgggtgg
      121 cagccccgcc ggaggccgtg aacaaaaaga cgtgcccggt ccgcagggtt tcatgcttca
      181 aaaccacggc ggcctgcttc agttccgcca catctgggta gcctccgaaa ccgccccgcg
      241 ctgatgcgcc gtatctataa ttcgattaca cattcacgcg ctcggcgctc gcccgatccg
      301 cccatttctt ctgcagccaa ctaaccgccg ggctcgcccc gccttcatcc cgggtcgacc
      361 cggcgaccgc gtttttcatc cctacctcct gttctcatca tgccacgaac ctgcctcctc
      421 ctccttgtca ccgccctctt ctccaccctc gccgtcgccg cccgcgacta cgatgtcgtt
      481 atttacggcg gcacctccgc cgccgtgacc gccgcggtcc aggccaaaaa aatgggcaaa
      541 tccgtgatca tcgtcggtcc cgacaagcat cttggcggcc tcagttccgg aggcctcggt
      601 tggaccgaca ccggcaacaa aaccatcatc ggcggcctct cgcgcgaatt ctatcaacgc
      661 gtttggcagc actacaaccg ccccgatgcg tggacgtggc aggacgccga tgactaccgc
      721 accggccgcc gcacgcgcac cgccatcgac gacgccacaa agaccgcgtg gaatttcgag
      781 ccgcatgtcg ccgagaaggt tttcgaggac ttcattcgcg aacacgatat cccggttcat
      841 cgcgacgaat ggctcgatcg agcgaaaggc gtgaagttgc aggatggccg catcgtttcg
      901 atcacgatgc tcagcggtca gacttacgcc ggaaagatgt tcatcgacgc tacctacgag
      961 ggcgacctca tggccgccgc cggagtcagc taccacgtcg gtcgcgaagc caaccgcgtt
     1021 tacggcgagg aatggaacgg cgtgcaggtt ggcatccttc accaccgtca ccacttcggc
     1081 ccttacaagg ccgcgagcag cctccccgcg ggtcacgcgc tcgagatcag cccctacgtc
     1141 gtgcccggcg atccgtcgag cggcgtcatt gcccgcgtca gcactgagcc gcccggcatc
     1201 cgcggcgagg gcgatcaccg cgtgcaggcc tactgcttcc gcatgtgttt gaccgatgtg
     1261 ccggaaaacc gcgtcccctt cgccaagccc gccggctacg atcctgcgca atacgaattg
     1321 ctcgcgcgca tctacgccgc gggctggaaa gaaaccttcg gcaagttcga tccgctcccg
     1381 aaccacaaaa ccgacacgaa caatcacggt ccgatgagca cggacaacat cggttacaac
     1441 tacgactacc ccgacgcctc ctacgagcgc cgcaaggaaa tcgttcagga gcacgccacc
     1501 taccagcagg gctggctcta tttcatcgcc aacgacccgc gcgtgcccgc cgaagtccga
     1561 aacgaaatgc agcgttgggg tcttccgaag gacgagttca aggacaacgg ccactggtcg
     1621 caccagctct acgtgcgcga agcccgccgc atgattggcg cctacgtcat gaccgaaaac
     1681 gaacttcgca aaaagcgtcc cacgcccgac tccgtcggca tgggttccta cacgatcgat
     1741 tcccacaacg tgcagcgcta catcacgccc gagggtcacg tgcagaacga aggcgacatt
     1801 ggcgtgtcca ccggcggtcc ctacgcgatc gcctacggtg cactcgttcc aaaacgcgaa
     1861 caagcctcca acctgctcgt gcccgtgtgt cttt
//
LOCUS       DASPLT010000300         1769 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_64834, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000300 DASPLT010000000
VERSION     DASPLT010000300.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1769)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1769)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1769
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_64834"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..442)
                     /locus_tag="VEA63_03160"
     CDS             complement(<1..442)
                     /locus_tag="VEA63_03160"
                     /inference="COORDINATES: protein motif:HMM:NF014851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD83015.1"
                     /translation="MNLARFLFAGPHRLRAFVGLFCVLATFAVRASTSDASPRTSSLL
                     DGRWEFQLDPDSVGIDQQWFAEGRPFPDHITVPGAWDAQGFGPETAKMRHNHIGKAWY
                     RRQIDIPAEPASGRTFLYFGGVCRSARVWVNGREAGSHLGYVSDF"
     gene            714..>1769
                     /locus_tag="VEA63_03165"
     CDS             714..>1769
                     /locus_tag="VEA63_03165"
                     /inference="COORDINATES: protein motif:HMM:NF014116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate binding domain-containing protein"
                     /protein_id="HYD83016.1"
                     /translation="MSAVFASEPSEAPRWQPRVAETTTNNLVPNSSFETGGFGWSSLG
                     QKVGWSGGLSSLFGNVTAAEKFEGDHSLEIALGPGKTATTYYDVLPAATVKQHAPLAM
                     NLGWITVTPGETYTLSAYMKANREGVPARLAMRVGGQIMPMPELTMEEKAVTLSTEWK
                     RYAFTFEATQADVFVGVGPDLSSQPDAEATVWIDAVQFEKRREPSAYVPRAAVEVAIE
                     SGRFGNVYSVDAPTNLIFRAVNRTEKAVELDVVLELRDYFDAALPESRQRVSVPAKGN
                     VSEEIALKVPGPGYYKLLATTTGGGVNEVMRFPLAVIHEYKKTDGPFGMNHPPATEEM
                     MQQLRKIGVVWGRNWALD"
BASE COUNT          360 a          470 c          640 g          299 t
ORIGIN      
        1 cgaaatcgga aacatagccg agatggctgc cggcttcgcg accgttgacc caaacgcggg
       61 cgctgcgaca aacaccgccg aagtaaagaa aggtgcggcc ggaggcgggc tcggcaggga
      121 tatcgatctg gcggcgatac caggccttgc cgatgtggtt gtgccgcatc ttggccgttt
      181 cgggaccaaa tccctgcgcg tcccaggcgc caggaacagt gatgtggtcg gggaatgggc
      241 ggccctcagc gaaccattgt tggtcgatgc cgacgctgtc gggatcgagt tggaactccc
      301 agcggccgtc gagcagcgac gaggtgcgag gcgaggcgtc gctggtggat gcgcgaaccg
      361 cgaaggtggc gaggacgcaa aacagtccga caaaagcccg cagccgatga ggaccggcga
      421 acaggaagcg agcaaggttc atggggaaaa aaggcgcagc ggagcagcgg cgagacgcgg
      481 actcgtggat ccgcgtaaag gatgcccggc ggcctcgctg aaacgctacg cctatgaaac
      541 caaacgttgg cacgattcgc tcagggaaga ggcctgcgct tgaatcggcg tgcaaggaag
      601 ccgaagtgtt tcccgagcca cgtttcaatt cccccgttca aacgatcatg aaatcaccga
      661 ttcctgtttt ccgtgcgccg cggcgcgtct tttttctgcc gcttgttacg ctcgtgagcg
      721 ctgtgttcgc cagcgaacca tcggaagcgc cgcgttggca gccgcgcgtc gcggagacga
      781 cgacgaacaa tctcgtgccg aacagcagct tcgagaccgg gggcttcggg tggtcgagtc
      841 ttgggcagaa ggtgggttgg agcggagggc tgtcgtcgtt gttcggcaac gtgaccgcgg
      901 cggaaaaatt cgaaggcgac cacagcctcg aaatcgcgct aggaccggga aagacggcga
      961 cgacgtatta cgacgtgctg ccggcggcga cggtgaaaca gcatgctccg ctggcgatga
     1021 atctcggctg gatcacggtg acgccgggcg agacctacac cttgtcggcc tacatgaaag
     1081 cgaaccggga aggcgtgccg gcgcggctcg cgatgcgcgt tggcggtcag atcatgccga
     1141 tgccggagct aacgatggag gagaaggcag tgacgctgtc gacggaatgg aagcggtatg
     1201 cgttcacgtt cgaggcgacg caggcggacg tgttcgtggg cgtcgggccg gacctttcct
     1261 cgcagcctga cgcggaggca acggtgtgga tcgacgcggt gcagttcgaa aaacgccgcg
     1321 agccgtcggc gtatgtgccg cgcgcagcgg tcgaagtggc gatcgagtca ggccgtttcg
     1381 ggaacgtcta ttcggtcgac gcgccgacga acctgatttt ccgggcggtg aatcggacag
     1441 agaaggcagt ggagttggat gtggtcttgg agctgcgcga ctatttcgac gcggcgctgc
     1501 cggaatcgcg gcagcgggtg agcgtgccgg cgaagggaaa tgtttcggag gagatcgcgt
     1561 tgaaggtgcc cggtccgggt tattacaagc ttttggccac gacgacgggc ggtggggtga
     1621 acgaggtgat gcggtttccg ctcgcggtga ttcacgagta taagaaaacc gacggaccgt
     1681 tcggcatgaa tcatccgccc gcgacggagg agatgatgca gcaactgcgg aaaatcggtg
     1741 tggtgtgggg tcgcaactgg gcgctcgac
//
LOCUS       DASPLT010000301         1087 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_291162, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000301 DASPLT010000000
VERSION     DASPLT010000301.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1087)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1087)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1087
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_291162"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..265
                     /locus_tag="VEA63_03170"
     CDS             <1..265
                     /locus_tag="VEA63_03170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD83017.1"
                     /translation="LELEFFPFPDLDPRQSSFSACVRVDDLDALYAEFLAAKLPDKSS
                     SIPRLTPPKTEPFGIRFFALIDLDGSLLRCIDNRSTEKLSPRT"
     gene            276..524
                     /locus_tag="VEA63_03175"
     CDS             276..524
                     /locus_tag="VEA63_03175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HYD83018.1"
                     /translation="MTTVRTKTFKSGNSEAVRLPKEIAFGEGVELVAVRSGDVLTLYP
                     AKKLTIQEMLAELDKLPAPDDVQERVEVEFLDRPGLYD"
     gene            521..907
                     /locus_tag="VEA63_03180"
     CDS             521..907
                     /locus_tag="VEA63_03180"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HYD83019.1"
                     /translation="MTFILDTSVAIPLRDGDKDVKVKLRSLVGTVAISAITRVELEGG
                     VYADPRSAAVRRRRLDLILAGTPTLDFTEAAAVAYDQIVAASGFSRRKIIDRMIAAQA
                     IVHRATLVTLNPTDFSDMPGLSSLAL"
BASE COUNT          190 a          370 c          312 g          215 t
ORIGIN      
        1 gctggagctg gagttcttcc cgtttcccga tctcgacccc cgccaaagtt cgttctcggc
       61 ctgcgttcgg gtcgatgatc tcgacgccct ctacgccgaa tttctcgcgg ccaagctgcc
      121 cgacaagtct tcgtcgatcc cgcgccttac gccgccaaaa acagagccat tcggcatcag
      181 attcttcgcc cttatcgatc tcgacggcag cctcctccgc tgcatcgaca accgctcgac
      241 ggagaagctt tcgccgcgga cgtaaacctg tgttcttgac cacggtccgc accaagacct
      301 tcaagagcgg caacagcgaa gccgtgcgcc tgccgaaaga gatcgctttc ggcgaaggcg
      361 tggaactcgt cgcggtgcgc tccggcgatg tgctgacgct ttatccggcg aagaaactga
      421 ctatccagga gatgctggca gaactcgata aactgcccgc gccggacgac gtgcaagagc
      481 gcgtcgaggt cgaatttctc gaccggccgg gcctgtacga ttgacgttta ttctcgacac
      541 aagcgtcgcg atcccgctgc gcgacggcga caaggatgta aaggtcaagc tccgttcgct
      601 cgtcggcacg gtagccatct cggcgatcac ccgcgtcgaa ctcgaaggcg gcgtctatgc
      661 tgatccgcgc agcgccgcag tgcgccgccg ccgccttgat cttattctgg ccggcactcc
      721 cacgctggac tttaccgaag ccgcggccgt cgcctacgac cagatcgtcg ccgcatccgg
      781 cttctcgcgc cgcaaaatca tcgatcgcat gattgcggcg caagcgatcg tacaccgcgc
      841 gacattggtg acgctcaacc cgaccgactt ctccgatatg cccggcttga gctcgctcgc
      901 gctgtaattc tcgggggccg cgcgcccgac gtcatttcgg acgcgcgacg cgcgatgggg
      961 aatccagtgg ccgtcgctcc gtcgctcgcc tctggattcc gggttcatcg ctgcgcgatg
     1021 ccccggaacg acgaatttgg gagtcccctg cccctggccc gatccggata ttggggtctc
     1081 gtgtatg
//
LOCUS       DASPLT010000302         2676 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_269747, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000302 DASPLT010000000
VERSION     DASPLT010000302.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 2676)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2676)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2676
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_269747"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1455
                     /locus_tag="VEA63_03185"
     CDS             <1..1455
                     /locus_tag="VEA63_03185"
                     /inference="COORDINATES: protein motif:HMM:NF024372.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HYD83020.1"
                     /translation="ELDYRVTPETTVNAYGDYFGSLVVMQSGIPARLKRYVLVTPANR
                     EFYVTERRIPTVAQTSLRNGERVQQWEMRDVPALPNEPRGLSTTEAGAYLNVSTFANW
                     TDLGRWYAEMLRPQLELDSALRDVASRMLAQYPAEEERIRAIHRFVLQNTHYVALEFG
                     IHSYKPYPVSQVYARRFGDCKDKASLMIALLRATGIEAQLALVRTRRLGGFAVEAASI
                     APFNHAIAYIPRYDLYLDGTAEYAGARELPLEDQGAMALTVSPEGESTLRQIPVTSPA
                     DNYTQRVVHAEIRSDGSIEFSGTAATRGEDAPFLRRDYEIAERQRESFRQTLADVFPS
                     VRVENVQVDGAHDLERDVTVSFRGLLDAFTGRNSLSLSNSWISRRYVQTLATLATRTQ
                     PLLLPAGWRTEERLHFTLPAGAANVSLPRDQLVVTPFGTAEFTYSRNDKEITIHTRVE
                     FSRTRVEPSEYPAFREFCRSLDFAFRQEAKVVLQ"
     gene            1653..>2676
                     /locus_tag="VEA63_03190"
     CDS             1653..>2676
                     /locus_tag="VEA63_03190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD83021.1"
                     /translation="MEASALQADDAAVLGFATRLCAAASSTADEDLCEIAAGRISELA
                     ANTRTFRDAVPRIQQLIASGGPHSGTLQLALVAAASDGAPEFDLLMKSRESGLLTDWK
                     IVGPLGRYANGDFDRALGPEQDSMRKKAYASRRVTSFQFIDGKVELPDASRSNGVFYA
                     AARTHLGSGGEWRVWVDSPGTLEVFVDGVSVLRKDDRSQQQPQVTWKLRRMEGGPHTV
                     VVKFLPSALPFRVAIMRPAAGVKRKNNKPALHAGPEGEYASVALRYWRGDYPGVGRRL
                     QRASRLGVASSLLLARAWQKATTSESSEARAALLQASAASPDIAALHLALAEQALASD
                     RIDEALS"
BASE COUNT          487 a          969 c          716 g          504 t
ORIGIN      
        1 gaactcgatt accgcgtcac tcccgagacc acggtgaacg cctatggcga ctacttcggc
       61 tcgctcgtcg tcatgcagag cggcattccc gctcgcctca agcgatacgt tctcgtcact
      121 ccagccaatc gcgaattcta tgtcaccgag cgacgcatcc cgaccgtcgc tcagaccagc
      181 ctcaggaacg gcgaacgcgt gcaacagtgg gagatgcgcg acgtccccgc gttgccgaac
      241 gaaccacgcg gcctctctac caccgaggct ggcgcctacc tgaacgtatc aaccttcgcc
      301 aactggaccg acctcggccg ctggtatgcc gagatgctcc gaccgcaact tgaactcgac
      361 tccgcacttc gcgacgttgc ctccagaatg ctcgcgcagt atcccgcaga ggaggagcgc
      421 attcgagcca ttcaccgttt cgtcttgcag aacacgcact acgtcgctct cgagttcgga
      481 atccacagtt acaagccata ccccgtatcg caggtctacg ctcgtcgctt cggtgattgc
      541 aaagacaaag ccagtctgat gatcgcgctt ctgcgtgcga ccggcatcga ggcacaactc
      601 gcccttgtgc gcacgcgccg cctcggcggt tttgccgtcg aagccgcttc catcgcgccg
      661 ttcaaccacg ccatcgccta catccccagg tacgatctat acctcgacgg cactgctgaa
      721 tacgctggcg cgcgcgaact gcccctcgaa gaccagggcg caatggctct caccgttagt
      781 cccgaaggcg aaagcaccct tcgtcagatt cccgtcacgt cgccggccga caactacacg
      841 cagcgcgttg ttcacgccga gattcgcagc gatggctcta ttgaattctc cggaaccgcc
      901 gcgactcgcg gtgaagacgc ccccttcctc cgccgtgact acgagatcgc cgaacgccag
      961 cgcgagtcgt ttcggcagac gcttgcagac gtcttcccgt ccgtccgcgt cgaaaacgta
     1021 caggttgatg gagcgcacga ccttgagcgc gatgtcacag ttagtttccg gggactgctc
     1081 gacgcattca ccggcaggaa ctccctttcg ctttcaaact cctggatctc gcgccggtac
     1141 gtgcagacgc ttgccactct cgcaacgcgc acccagccgc tgcttctgcc agccggatgg
     1201 cgcaccgagg aaagactgca tttcacgctc ccggccggcg ccgccaatgt aagcctgccg
     1261 cgcgaccagc tcgtcgtcac gcctttcggc actgccgagt tcacttatag ccgcaacgac
     1321 aaggagatca cgattcacac tcgcgtcgag ttcagccgca cccgcgtcga gcccagcgaa
     1381 tacccggcct tccgtgaatt ctgccgctcg ctcgacttcg ccttccgcca ggaagccaag
     1441 gtggtgttgc aatgaagcgt tacttgccgc agatcatcgc tgtggttctg cttgcagtca
     1501 ctgccgtcgc ccagccctcc gctcgcccgg tcgatcgcgc atacaccgca gccgacgcgc
     1561 tcttccgccg gtctgacgtg agcaccgcaa tgcgtgaagc ttcgctggca cttcaagtcg
     1621 atcgccacaa cacgcgtgcg ctcttcgttg ccatggaagc ctctgcgctc caggccgatg
     1681 acgccgcggt cctcggcttc gccactcgcc tctgcgccgc cgcgtcatcc acggccgacg
     1741 aagacctctg cgagatcgcc gctggccgca tctccgaact cgccgccaac acgcgcacgt
     1801 tccgtgacgc ggtcccgcgc attcagcaac tgatcgcctc tggaggtcct cacagcggca
     1861 cgcttcagtt ggcactcgtc gccgccgcaa gcgatggtgc acccgaattc gatctcctga
     1921 tgaagtcccg cgaatccgga ctccttaccg actggaagat cgtcggtccg ctcggacgct
     1981 acgcaaatgg cgatttcgac cgcgcgctcg gtcccgaaca ggacagcatg cgcaagaaag
     2041 cctacgcctc tcgccgcgtc accagtttcc aattcatcga cggcaaagtc gagttgccgg
     2101 acgccagtcg ctcgaacggc gtcttctacg cggctgctcg cacccatctc ggctccggcg
     2161 gtgaatggcg tgtgtgggtt gacagtcccg gaacccttga ggtcttcgtt gacggagtct
     2221 ccgttctccg caaggacgat cgcagccagc aacagcctca agtcacgtgg aagcttcggc
     2281 gcatggaagg cggcccgcat acagttgtcg tcaagttcct tcccagcgca ctgccgttcc
     2341 gcgtcgccat catgcgtcct gccgcgggcg tcaagcggaa gaacaataaa cctgcactcc
     2401 acgcaggccc cgaaggcgaa tacgcttccg tcgcccttcg ctactggcgc ggcgactacc
     2461 ccggagtcgg tcgccggctc cagcgcgcgt ccagactcgg tgtcgcttct tcgctgttac
     2521 tcgctcgtgc atggcaaaag gcgaccacct ctgaatccag cgaagctcgc gctgccctgc
     2581 tccaggcctc cgccgcctcc cccgatatag ccgccctaca tttggctctc gccgagcagg
     2641 ccctcgcctc cgatcgaatc gacgaggcgc tttcca
//
LOCUS       DASPLT010000303         2009 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_167657, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000303 DASPLT010000000
VERSION     DASPLT010000303.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 2009)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2009)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2009
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_167657"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1038)
                     /locus_tag="VEA63_03195"
     CDS             complement(<1..1038)
                     /locus_tag="VEA63_03195"
                     /inference="COORDINATES: protein motif:HMM:NF018743.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1343 domain-containing protein"
                     /protein_id="HYD83022.1"
                     /translation="MLGIDVLEAEGFAPVKGKRVGLLTHPAGVNRHGVSTIQILHQAP
                     GIQLVALYAVEHGIDNAMPAEAPFPDTIDRRTGLPVFSLYNGKTKKFLPTPEQLKPID
                     VLVIDLQDIGTRSYTFSGAMKTAIEACFENNKDVVVLDRPNPLGGLKVGGPPLDADLI
                     TDVGKFRVPYVHGLTIGELAQLAYALRPPGGLAVSDAARARGKLTVVPMRGWKRSMRW
                     PDTGLAFVPTSTRIQDFAAVIGYPMTGLGCIAGGFRHGIGPAYPFRGVHHADVKLHVL
                     DRKLSALDLPGVHFRRVVGPPRKDSSRGTGLYIDITDYDAWQPTDLNFRLMKLAAEFA
                     PRNPFAALRGTP"
     gene            1490..>2009
                     /locus_tag="VEA63_03200"
     CDS             1490..>2009
                     /locus_tag="VEA63_03200"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HYD83023.1"
                     /translation="MRNERVHANTGRFAHPLRGQVVVITGASSGIGRATARAFAECGA
                     RVVLAARNEDALHDVVGECARRGAHAIVQRTDVGDAKAVEELVRVAAEAFGRIDVWIN
                     NAGVGLFGPFTHADMEMHRRVVETNLFGAMNGAAAVLPYFMEQGRGTMITNISVGAFA
                     PVPFAAAYTAGKF"
BASE COUNT          385 a          548 c          731 g          345 t
ORIGIN      
        1 gggcgtgccg cgcagtgcgg cgaatggatt gcgcggcgcg aactcggcgg ccagtttcat
       61 caaccggaaa ttcaggtccg tcggctgcca cgcatcgtag tcggtgatgt cgatgtagag
      121 accggtgccg cgcgaactgt ctttccgtgg cgggccgacg acgcggcgaa agtgcacgcc
      181 aggaaggtcg agcgcgctca gcttgcgatc gaggacgtgg agcttcacgt cggcgtgatg
      241 gactccacga aatggatacg cgggtccgat gccgtggcga aagccgccgg cgatgcagcc
      301 gaggccggtc atcggataac cgatgacggc ggcaaaatcc tgaatgcgcg tcgacgtggg
      361 cacgaacgcg agtccggtgt ccggccaccg catcgaacgt ttccaaccgc gcatcgggac
      421 gaccgtgagt ttgccgcgtg cgcgcgccgc atcggaaacg gcgagccctc ctggcggccg
      481 caatgcatac gcgagctgcg cgagttctcc gatcgtgagc ccatgaacgt agggcacgcg
      541 gaatttgccg acatcggtga tcaggtcggc gtcgagcggc ggaccgccga ccttgaggcc
      601 gccgagaggg ttcgggcggt cgagcacgac gacgtccttg ttgttttcga agcaggcctc
      661 gatcgcagtc ttcatcgcgc cgctgaacgt atagctgcgc gtgccgatgt cctgcaggtc
      721 gatcacgagc acatcgatcg gtttcaattg ctcgggcgtg gggagaaatt tttttgtttt
      781 gccgttgtag agcgagaaaa ccggaagtcc ggtccgtcga tcgatcgtgt cgggaaaggg
      841 tgcttccgcg ggcatcgcgt tatcgatgcc gtgctcgacg gcgtagagtg cgacgagctg
      901 aatgccgggc gcctggtgca aaatctgaat cgtcgacacg ccgtggcgat tcacgcccgc
      961 gggatgggtg aggaggccaa cccgtttgcc tttcacgggc gcgaaaccct cggcctccaa
     1021 cacgtcgatg ccgagcatca cgggaaacgg cgccgacggc ggctgagttg gaagggcgac
     1081 ggcgcgtgcc gcggtgggtg agtgaccggc tgcgcaaccg gacagaatca gaagcaacgc
     1141 gaacgccggc gggagaaagc aagtgagcga cagggagcgg gaagcgccgg acacgagagc
     1201 acaaggtgcg gtgcgacgac gcgcggtcga gtcgtttcga gcggcaaggc ccgcgctcgc
     1261 accgcggaat tagtcggaat caatcacacc gaaaaaaggc cgtggtgtgg aatacagagg
     1321 acacggagcg atgacacaga agtcacagag caaggcaggt gtttggttct atgagctctg
     1381 gattggagcg tgcacactct gccgcaaaat aggcccggga aagttttccc cacgggttct
     1441 tcggcggtaa tccgaatgac gggcgccggt tgacggtgta ggttggcgga tgagaaacga
     1501 acgtgttcat gccaataccg gaaggttcgc ccatccgctg cgcggtcagg tggtcgtcat
     1561 caccggcgcg tcgagcggga tcggtcgcgc gaccgcccga gcgtttgccg agtgtggagc
     1621 gcgcgtggtg cttgcggcgc gcaacgagga tgcgttgcac gacgtcgtcg gcgagtgcgc
     1681 gcggcgagga gcccacgcga tcgtgcagcg aacggatgtg ggcgatgcga aggcggtgga
     1741 agaactcgtg cgcgtggcgg cggaggcgtt tggacgaatc gacgtgtgga tcaacaatgc
     1801 gggcgtgggg cttttcgggc cgtttacgca cgccgacatg gaaatgcacc ggcgagtggt
     1861 ggagacgaat ttgttcggtg cgatgaacgg cgcggcggcg gtgctgcctt atttcatgga
     1921 gcaaggccgt gggacgatga tcacgaacat ctcggtcggc gcgtttgcac cggtgccgtt
     1981 tgcggcggct tacaccgcgg gaaagtttg
//
LOCUS       DASPLT010000304         2106 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_228405, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000304 DASPLT010000000
VERSION     DASPLT010000304.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 2106)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2106)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2106
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_228405"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1776
                     /locus_tag="VEA63_03205"
     CDS             <1..1776
                     /locus_tag="VEA63_03205"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HYD83024.1"
                     /translation="PGPNAGACGSAFALRKRVVVEDTEADQCFLLFREVARAAGFRAV
                     HSTPVLNRAGDILGIVSVHFDDPRRPSQAEMQLADMCARYAADAIESTRHQDALRESE
                     ERFRTLADSAPVLIWVNGPDQCEYVNRYYREFCGIDTVGPFDWSAVLHPEDRQAYVEA
                     YVAAAGERSHFAAQCRVRRGSGDYRWVQSVGMPRFAPTGEWLGYVGCSVDITDLKSAG
                     DQLREADRRKDEFLAVLAHELRNPLAPIRNGLHLLRAVDPQSSGAEQMRAMMERQVNH
                     LVRLVDDLMELSRITTGKLELRKEPTELAAVVGSAVESSRPLMDAKGHRLRIHLPAEA
                     LRLNADPVRLAQVIANLLNNAAKYTHDRGEIVLETRRDADCAVVSVRDSGVGIPPDML
                     AHIFDKFTQVDTSYGRAQGGLGVGLSLARSLVELHGGSIEARSEGPGQGSEFIIRLPL
                     AAWRNTAASVAPAVQGGARPLPHASRRILVVDDNADAADSLGMLLEHMGHQVHVVNDG
                     ESALAAARAGRPDVMFLDISMPGMDGYTVASRLRMDPRLAKTRIVAMTGHGQEDDRRK
                     SREAGFDAHVVKPVAPESLGELLKM"
BASE COUNT          393 a          669 c          696 g          348 t
ORIGIN      
        1 cccggcccga acgccggcgc ctgcggaagc gcgttcgccc tgcgcaagcg cgtagtagtg
       61 gaggacacgg aagcagacca gtgcttcctc ctattccgcg aagtcgcgcg cgcggccggc
      121 ttccgggcgg ttcacagtac gccggtcctc aaccgcgccg gcgatattct cggcatcgta
      181 tcggtccact tcgacgatcc tcgacgccca agccaggctg agatgcagct tgccgacatg
      241 tgcgcgcgct acgccgcgga cgccatcgag tcgacccgcc atcaagacgc tttgcgcgag
      301 agcgaggagc gcttccgtac gttggccgac agcgcgcccg tgctgatctg ggtgaacggt
      361 cccgaccaat gcgagtacgt gaatcgctac taccgcgagt tctgcggaat cgacacggtt
      421 ggtccgttcg actggtcggc ggtcctgcat cccgaggacc gccaagcata tgttgaggcg
      481 tacgttgcag ccgcgggaga gcggagccac ttcgcggcgc aatgccgcgt ccggcgagga
      541 agcggcgatt accgctgggt ccagtcggtc ggaatgcctc ggtttgcgcc gaccggcgaa
      601 tggctcggct acgtgggctg ctccgtcgac atcacggacc taaagagcgc cggagaccag
      661 ctgcgcgagg ccgatcggcg caaggacgag ttcctcgccg tgctcgccca tgagctgcga
      721 aatccgctcg cgcccatacg caacgggttg cacctgctgc gcgccgtaga tccccagagc
      781 agcggcgcgg agcagatgcg ggcgatgatg gagcggcagg taaaccacct cgtccgcctc
      841 gtcgacgatc tgatggagct atcgcggatc accaccggca agctcgagct aagaaaggaa
      901 ccgaccgagc tcgccgctgt cgtcggcagc gctgtggaat ccagccgtcc gctgatggac
      961 gcaaagggcc accgcctgcg catccacctt ccggcggaag ctctacggtt gaatgcagat
     1021 cccgtccgcc tcgcccaagt catcgcgaac ctgctcaaca acgcggcgaa gtacacccac
     1081 gataggggcg agatcgtgct cgaaactcgt cgcgacgccg actgtgccgt ggtaagcgtg
     1141 cgggacagcg gagtaggcat tccgcccgac atgctggcgc acatcttcga caagttcact
     1201 caggtcgaca cgagctacgg tcgggcgcag ggcgggctgg gtgtggggct ctcgcttgcc
     1261 cgctcgctgg tcgagctgca cggcggcagc atcgaggcac gcagcgaagg tcctggccaa
     1321 ggcagcgagt tcatcatccg cctcccgctc gccgcgtggc gcaacaccgc ggcctccgtc
     1381 gcaccggccg tgcaaggcgg cgcgcgtccc ctaccgcacg cgagccgtcg catactcgtc
     1441 gtcgacgaca atgcggacgc cgccgacagc ctcggcatgc tcctcgagca catgggacac
     1501 caggtgcatg tcgtcaacga cggcgaatcg gcccttgccg ccgcgcgggc gggacgaccg
     1561 gacgtgatgt tcctcgatat aagcatgccg gggatggatg gctataccgt cgcgagtcgt
     1621 ctgaggatgg atccgaggct ggcaaagacc aggattgtcg caatgaccgg ccatggccag
     1681 gaagacgatc gccgcaagtc gcgcgaggcc gggtttgacg cccacgtcgt gaagcctgtc
     1741 gcgccggaaa gtctgggcga gcttctgaag atgtaagagc cgcgagggct ccggcggttg
     1801 gggcgaattg gggctaaacc gtgggctttg cgggctactg gagagcaccc ggtttttact
     1861 ctcacagcga agggcaatca ggggggtagt gccaacgcgg gttcgattcc cgcctccacg
     1921 tcagaactac ggttcggtgg accaacggtt ttctgcagtt aactgcaaaa ggccgttgtt
     1981 aggagcttcg ataattgaaa agcgcgctga ccgtagtctg gatgcttgcc ggagggccaa
     2041 ttgcgctggc agtcctcaat ttgcgttgga ctactggccc caaaatggcc cccggaaaag
     2101 aaaagg
//
LOCUS       DASPLT010000305         1064 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_269760, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000305 DASPLT010000000
VERSION     DASPLT010000305.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1064)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1064)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1064
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_269760"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..490
                     /locus_tag="VEA63_03210"
     CDS             <1..490
                     /locus_tag="VEA63_03210"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HYD83025.1"
                     /translation="RRPRLEAIALTGAGAYSGGIERLPGEIDYAPGAVRHEYGTRNAG
                     LVAGLAEAVRLQEEIGRERIAAHGRELAAQLRDGLTGVKDVTLLTPRPDGLRASITTF
                     RHARANAEKMFGYLIEQHRLRCRPVTEQGIEATRISTHVFTSHADCERVIVGVRAAAR
                     VL"
     gene            520..>1064
                     /locus_tag="VEA63_03215"
     CDS             520..>1064
                     /locus_tag="VEA63_03215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD83026.1"
                     /translation="MVKIKLYLTLIVLLGGIARGATAPKHEMYLCATTSLNYIVGAKM
                     VTASGLYHREADGSYRHFGVNFPGIFSLAIDPRDSQRLYVASLNGALCSADGGKTWRI
                     GTSWDMTEPKDIFVDPNAPDNIYLALPDGIAVSPDRGVTWPRRENGLPERGKYTQVVK
                     VDRMRAGRALAGCESGIYVTE"
BASE COUNT          184 a          302 c          381 g          197 t
ORIGIN      
        1 ccgtcggccg cggctcgagg cgatcgcgct gacgggcgcc ggcgcctatt cgggtgggat
       61 cgagcgtttg ccgggcgaga tcgattatgc gccgggagcg gtgcggcacg aatatggcac
      121 gcgcaacgcg gggctggtcg ctggcttggc ggaagcggtg cgattgcagg aagagattgg
      181 gcgcgagcgg atcgccgcgc atggccgcga actcgcggcg caattgcgcg atgggctcac
      241 gggagtgaag gacgtcacgt tgttgacgcc gcggcctgac gggcttcgcg cgtcgatcac
      301 gaccttccgg catgcgcgcg cgaacgccga gaagatgttc ggatatctga tcgaacagca
      361 tcggttgcgc tgccgtccgg tcacggaaca agggatcgag gcgacgcgca tctcgacgca
      421 cgtgttcacc tcgcacgcgg actgcgagcg cgtcatcgtc ggcgtgcgcg cggcggcgcg
      481 agttctctag gcctgtgcgg tctcgtttcg attcattcca tggttaaaat aaaactttat
      541 ctgacactga tcgtgttgct cggcggcatc gctcggggag cgacggcgcc gaagcacgag
      601 atgtatctgt gtgccaccac cagcctgaac tacatcgtcg gagcgaagat ggtcaccgcg
      661 agcgggctgt atcaccgcga ggccgacggc agctatcggc acttcggggt taattttccc
      721 gggatcttca gtctcgcgat cgacccgcgc gactcgcagc ggctctacgt tgcttcgttg
      781 aatggcgcgc tgtgcagtgc cgacggcggc aagacgtggc ggattgggac gagttgggac
      841 atgacggagc cgaaggatat tttcgtcgat ccgaacgcgc cggataacat ctatcttgcg
      901 ttgcccgatg gcatcgcggt gtcgccggat cgcggtgtga cgtggccgcg ccgcgagaac
      961 gggttgccgg aacgcggcaa atacacgcaa gtcgtgaaag tcgatcgcat gcgtgccggg
     1021 cgcgcgctcg cgggctgcga gtcgggcatt tatgtgacgg agaa
//
LOCUS       DASPLT010000306         1236 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_105339, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000306 DASPLT010000000
VERSION     DASPLT010000306.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1236)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1236)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1236
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_105339"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..567
                     /locus_tag="VEA63_03220"
     CDS             <1..567
                     /locus_tag="VEA63_03220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HYD83027.1"
                     /translation="NQNVLDHSIETASLASMIASEVGLDPNVAKRAGLLHDLGKAVSG
                     DLEGSHAHIGAEFIKRYGETPIVVNAVAAHHEEVKPETVYAGIVILADTISATRPGAR
                     AESMAGYVQRLARLEKLAASIEGVQQSFAIQAGREIRVVVSPQTVTDDRAREIAKELR
                     KRIEEELQYPSTIKITVIREQRFTETAT"
     gene            677..1042
                     /gene="queF"
                     /locus_tag="VEA63_03225"
     CDS             677..1042
                     /gene="queF"
                     /locus_tag="VEA63_03225"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preQ(1) synthase"
                     /protein_id="HYD83028.1"
                     /translation="MSDPKILETFPNPAPHRDFTIEHTHHEFTSVCPMTGHPDFANIT
                     VRYVADKTCVELKSLKLYFHAFRNEGIFFEAATNKICDDLGATLKPRSLTIISDWKAR
                     GGFTSRVTAEWKPRAKSRK"
BASE COUNT          248 a          320 c          436 g          232 t
ORIGIN      
        1 aaccagaacg tgctcgatca ttcgattgaa acggcgtcgc tcgcgtcgat gatcgcgagc
       61 gaggtcgggc tcgatccgaa tgtcgcgaag cgcgcagggt tgttgcacga tctgggcaag
      121 gcggtgagcg gcgatctcga aggaagccat gcgcacatcg gcgcggagtt catcaagcgt
      181 tatggcgaaa ccccgatcgt ggtgaacgcg gtagcggcgc atcacgagga agtgaagccc
      241 gagacggtgt atgcgggcat cgtgattttg gcggatacga tttcggcgac gcggccgggc
      301 gcgcgcgccg agtcgatggc gggttacgtg cagcggctgg cgcggctgga gaaactcgcg
      361 gcgtcgatcg agggcgtgca gcaatcgttt gcgatccagg ccggccgcga gattcgcgtg
      421 gtggtttcgc cacagacggt gaccgacgat cgggcgcggg agattgcgaa ggagctgcgc
      481 aagcggatcg aggaggagct gcagtatccg agcacgatca agatcacggt gatccgtgag
      541 cagcggttta ccgagacggc gacgtgacgg ggcggttttg ggttgtgggt ttggagtttt
      601 gagtttcgtg ccggggttcg cggctggaca atctccagcg cgaaactcaa aacccaaaac
      661 tcaaaacctt tccgccatgt ccgatccgaa gattctcgag acttttccca atcctgcgcc
      721 gcatcgcgat ttcacgatcg agcatacgca tcatgagttc acgtcggtgt gtccgatgac
      781 ggggcatccg gatttcgcga acatcacggt gcgttacgtc gcggacaaaa cctgcgtgga
      841 gctgaagtcg ttgaagctgt atttccacgc gtttcgcaac gaagggattt tcttcgaggc
      901 ggcgaccaat aagatttgcg acgaccttgg cgcgacgctg aagccgcgga gcctcacgat
      961 tatttcggat tggaaagcgc gcggcggatt tacgtcgcgg gtgacggccg agtggaaacc
     1021 gagggcgaag tcgcggaagt gagctaaagc gattcaggcg taggtggcgc gcttgtcgcg
     1081 ccatgtgttg cgcgcaagca cgcaacctac ggaagcacgc aggctgaagc ctgttgcgaa
     1141 cgatcggaag caggcacgcg cggaggcgtg ccgctacgcg agcgcggcga tcacttgcgc
     1201 gtggagttcg ggtgtggcgc aggcgatcgt ggactc
//
LOCUS       DASPLT010000307         1065 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_209015, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000307 DASPLT010000000
VERSION     DASPLT010000307.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1065)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1065)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1065
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_209015"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(257..976)
                     /gene="nth"
                     /locus_tag="VEA63_03230"
     CDS             complement(257..976)
                     /gene="nth"
                     /locus_tag="VEA63_03230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HYD83029.1"
                     /translation="MRSRNRESGGEIAFACRGRFLGAMTRQERADYVDRRLAELYPDP
                     AIPLDHRDPFTLLVAVLLSAHTTDKSVNRVTPELFALADTPEKMARVPVEELQRIIRP
                     VGLSPAKSKALAAMSRMLVEKHGGVVPRTFEALEELPGVGHKTASVVMTQAFGVPAFP
                     VDTHIHRLAQRWKLTDGRSVEQTERDLKVLFPREHWNKLHLQIIYYGREHCTARGCDG
                     TVCEICRTLFPERKRAVKTRK"
BASE COUNT          198 a          391 c          262 g          214 t
ORIGIN      
        1 ctgagctaag cgtccgactc gggaggcgag aacgtggaca gccccgcccc gcgaatcaac
       61 cctgttttgc gttcgaagcc gatccgcacc ttcccgacgg agatcagcca caaaaaggca
      121 cgagaagaca caaaaatacc gaaccgcttc tttcctttct tcgtgccttc tcgtgccttt
      181 tcgtggccga aacttctgcg ccttttctgc cttttgtggc ccaacaactc gcccgcccct
      241 ccgcgcgcgc cgcgctttac ttccgtgttt tcaccgcacg ttttcgctcg ggaaacagcg
      301 ttcggcaaat ctcgcacacc gtgccatcgc acccgcgagc cgtgcaatgc tctcgtccgt
      361 agtagatgat ctgcaggtgc agcttattcc agtgctcgcg cggaaacagc accttcaaat
      421 cccgttcggt ctgctcgacg cttcgcccgt ccgtcagctt ccatctttgc gcgagccgat
      481 gaatgtgcgt atccaccgga aaagccggca cgccaaacgc ctgcgtcatc accacactcg
      541 ccgtcttgtg tcccacgccg ggcagctcct cgagtgcttc aaacgtccgc ggcacgacgc
      601 cgccatgttt ctcgaccagc atccgtgaca tcgcggccag tgctttcgac tttgcgggcg
      661 agagccccac cggccgaatg atccgctgca gctcctcgac cggcacgcgt gccatcttct
      721 cgggcgtatc cgccagcgca aaaagctccg gagtcacgcg attcaccgac ttatccgtcg
      781 tgtgcgccga caacaacacc gccaccaaca acgtaaacgg atcacggtga tccaacggga
      841 tcgcaggatc gggatacaac tccgcaagtc gtcggtccac gtaatcggct ctctcctggc
      901 gcgtcatcgc gccgagaaaa cgtccacgac acgcgaatgc aatctcgccg cccgattctc
      961 gattgcgcga cctcatgcgg gccgtagcct cggcgctttc tcatgtcctc gcccagcgac
     1021 cttctcgccc cgctcgacac cttctctcgc cgccacactg gcgcc
//
LOCUS       DASPLT010000308         1449 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_188011, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000308 DASPLT010000000
VERSION     DASPLT010000308.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1449)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1449)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1449
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_188011"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..133)
                     /locus_tag="VEA63_03235"
     CDS             complement(<1..133)
                     /locus_tag="VEA63_03235"
                     /inference="COORDINATES: protein motif:HMM:NF026192.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HYD83030.1"
                     /translation="MTDIDFSKLSRMQKLATFLVVIGPEAAASVLKQFDEPDIELLCR
                     "
     gene            complement(149..>1449)
                     /gene="fliF"
                     /locus_tag="VEA63_03240"
     CDS             complement(149..>1449)
                     /gene="fliF"
                     /locus_tag="VEA63_03240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="flagellar basal-body MS-ring/collar protein
                     FliF"
                     /protein_id="HYD83031.1"
                     /translation="SGDGVGFEIFDKGQFGLSDFVQRTNYLRAVQGELARTIMQLQGV
                     RAARVMIVQPENRLLLTDQGVKSTASVFIDVGGGRLDPDQVNAVRHLVANAVQGLAPD
                     QVAVVDNRGRVLSEELKQDPTLGTASSQIRYRQQVEDYFSKKVETMLAAVIGPGNAVV
                     RVSAEIDTEAVTMHEEKFDPEGQVVRTQTSTEDTSSSAESRSAGGVVGVSANVPEKNP
                     GTETSRPVSNTEQNRKNRTTTYEINRTTLNTTRNPGTVKNLTAAVFIAPRPAPVAVDA
                     EGVAQPTGEAPKRTPEELNALRQLVVNALGLKPIAGQPLDSIVSLQEMPFQAAEQIPA
                     QIAAIGNESRWQGWVEAASRWAGVAGAAIVLLIFWRMLGRQKPEPVPVEVLSLTPEAA
                     SRALPTSNVTPDLLNDLIRQKPANIGVALRDWVSAGASKN"
BASE COUNT          233 a          421 c          510 g          285 t
ORIGIN      
        1 cgcggcagag cagctcgatg tcgggctcgt cgaactgctt gagcacgctc gcggcggcct
       61 cgggtccgat gaccacgagg aaggtcgcaa gcttctgcat gcggctgagt ttgctgaaat
      121 cgatgtcggt catggaggag cccggcgctt agttcttgct cgcgccggcg gaaacccagt
      181 cgcgaagggc gacaccgatg ttggccggtt tctggcggat gagatcgttc aacaaatcgg
      241 gtgtgacgtt gctcgtcggc agcgcgcgcg acgcggcctc gggcgtgagg gacaacactt
      301 cgaccggaac cggctcgggt ttttgacggc cgagcatacg ccagaaaatc agcagcacga
      361 tcgcagcacc ggcgacgcct gcccaacgac tcgcggcttc gacccaaccc tgccagcggc
      421 tctcgttgcc gatcgcggcg atttgcgcgg gaatctgctc cgcggcttgg aacggcattt
      481 cctggagaga aacgatcgag tcgagcggct gaccggcgat gggcttgagg ccgagcgcgt
      541 tgacgacgag ctggcgcagc gcgttgagct cttccggcgt gcgcttcggc gcttcgcccg
      601 tcggctgggc aacgccttcg gcgtcgactg cgaccggagc cggccgcggc gcgatgaaga
      661 cggccgcggt gagatttttc acggtgccgg gattgcgcgt ggtgttgagc gtggtccggt
      721 tgatctcgta agtcgtcgtg cggttcttgc gattctgctc ggtgttcgag acggggcgcg
      781 acgtttcggt gccgggattt ttttccggga cgtttgcgct gacgccgacg acaccgccgg
      841 cggaacgcga ttcggcggac gaagacgtgt cttcggtcga cgtctgcgtg cgcacgacct
      901 ggccttcggg gtcgaatttc tcctcatgca tcgtgaccgc ttctgtatct atttctgcgg
      961 atacacggac gacggcgttg ccgggcccga tgacggcggc gagcatcgtt tcgaccttct
     1021 tggaaaagta atcttcgacc tgctggcggt agcgaatctg cgacgaagcg gtgccgagcg
     1081 tcggatcctg tttcaattcc tcggagagca cgcgaccgcg gttatcgacg acggcgacct
     1141 ggtccggtgc gaggccttgc accgcgttgg cgacgaggtg acgcacggcg ttgacctgat
     1201 cgggatcgag acggccgccg ccgacgtcga tgaaaacgga tgcggtggac ttcacgcctt
     1261 gatcggtgag aaggagacgg ttctccggtt gcacgatcat gacgcgcgcg gcgcgcacgc
     1321 cttggagctg catgatggtg cgggcgagtt cgccctgcac ggcgcgaagg tagttcgtgc
     1381 gttgcacgaa gtcggagagg ccgaactggc ctttgtcgaa aatttcgaag ccgacgccgt
     1441 cgccgctgg
//
LOCUS       DASPLT010000309         1045 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_188017, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000309 DASPLT010000000
VERSION     DASPLT010000309.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1045)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1045)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1045
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_188017"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..186)
                     /locus_tag="VEA63_03245"
     CDS             complement(<1..186)
                     /locus_tag="VEA63_03245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HYD83032.1"
                     /translation="MVSSSVSEPALSSSAAALSSDIVADALALRHSADAEFYRSVLES
                     LGEGVMITDAQWRIQYAN"
     gene            433..>1045
                     /locus_tag="VEA63_03250"
     CDS             433..>1045
                     /locus_tag="VEA63_03250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007359568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HYD83033.1"
                     /translation="MTSLPNTHSLLLLNFSPRGGRSGSRKLADEYLASWRAAHPAGKS
                     IVREIGANPPPAISEAWIAGAFTPPEQHTPAMREAIGVSDAFVDELLAATDIVIATPI
                     YNFNIPAALKLWIDQIVRAGRTFAVDASGYHGLTGGRSVKVLVSSGGDLRPGQPAAAM
                     NFAEPYLRGVFGFIGIDRVEFVYAPNQSAADAQREVAFTQAVDA"
BASE COUNT          193 a          293 c          357 g          202 t
ORIGIN      
        1 gttcgcatac tggatgcgcc actgtgcgtc ggtgatcatc acgccctcgc cgaggctctc
       61 gaggacgctg cgatagaatt ccgcgtcggc ggagtgacgt agcgcgagcg catcggcgac
      121 gatgtcggag ctcagcgcgg cagcggagga ggacagggca ggttcgctga cagatgagga
      181 gaccatggcg ggagaaacga gagaagactg gctggcagcg aacgacggtc atccgctggc
      241 gcaaccacga aagctcgttt catggcaacg aatgttcgtt gataacgagt tttcgttgcg
      301 agctaaggag tgctcggtag gtgagcgatt gttttgtatc tggttttttt aacggggcat
      361 aagtctcgtc cgttagccgg ttttggaggc tggcacgcat taggcgaaag ggaggggaaa
      421 ccgatttaaa ccatgacttc ccttcccaat actcactcgc ttttgcttct gaacttcagc
      481 ccgcgcggcg ggcgctctgg cagccggaag ctggctgacg aatatctcgc ctcgtggcgg
      541 gccgctcatc cggccggcaa gagcatcgtg cgcgagatcg gcgcgaaccc gccgccggcg
      601 atcagcgagg cgtggatcgc gggcgctttc acgccgcctg aacaacacac gccggcgatg
      661 cgcgaggcga tcggggtgtc ggacgcattt gtcgacgaac tgctcgcggc gaccgacatc
      721 gtgatcgcga caccgatcta taatttcaac attcccgcgg cgctgaagct gtggatcgac
      781 cagatcgtgc gcgcgggccg cacgtttgcc gtcgatgctt cgggctatca cggcctgacc
      841 ggcggtcgca gcgtgaaagt gctggtgagt agcggcggcg atttgcggcc cgggcagccg
      901 gcggctgcga tgaatttcgc ggagccttat ctgcgcggcg tgttcggatt tatcggaatc
      961 gaccgcgtcg agttcgttta cgcgccgaac caatcggcgg cggacgcgca gcgcgaagtc
     1021 gcgttcacgc aggcggtcga tgccg
//
LOCUS       DASPLT010000310         1117 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_64940, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000310 DASPLT010000000
VERSION     DASPLT010000310.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1117)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1117)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1117
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_64940"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..927)
                     /locus_tag="VEA63_03255"
     CDS             complement(<1..927)
                     /locus_tag="VEA63_03255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012377107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase ligase N-terminal
                     domain-containing protein"
                     /protein_id="HYD83034.1"
                     /translation="MSLKEYVRKRDFKKTAEPKGGRVTGGAAHRFVIQKHAASRLHYD
                     FRLEVDGTLKSWAVPKGVPYAKGERRLAVHVEDHPVSYIDFEGTIPQGQYGGGTVMVW
                     DRGTFEPLSENPSKELEGGKLHFVLKGKKLRGEWYLVRLRGGDEWLLIKGGEDMKPLS
                     KKADDTSVISGKSMAALSKGEGRVWQSNRTDGVSSFKQSIARAAHAAETKAKAKTKTK
                     PGAKRPAKPSSKKNGVDSAAKGAALRRTKSKLTPEFIEPMKALLVAEPPPGEWIYEIK
                     FDGFRAMAYRDRRGTRLLSRNNKDFAAKFPEVF"
BASE COUNT          180 a          404 c          294 g          239 t
ORIGIN      
        1 gaacacctcg ggaaatttcg ccgcgaaatc cttgttgttc cgcgacagca gccgcgtgcc
       61 gcgccgatcg cgatacgcca tcgcgcgaaa gccatcgaac ttgatctcgt agatccactc
      121 gcccggcggc ggctccgcga cgagcagcgc cttcatcggt tcgatgaact cgggcgtgag
      181 tttcgacttc gtccgccgga gagccgcgcc cttcgccgcg ctatcgacgc cgtttttttt
      241 tgaagatggc ttcgccgggc gcttcgcacc cggcttcgtt ttcgttttcg cctttgcctt
      301 cgtttccgct gcatgcgccg cccgcgcgat cgattgcttg aacgacgata caccgtcggt
      361 ccgattcgat tgccacacgc ggccttctcc cttcgaaagc gccgccatcg atttccccga
      421 gatcaccgaa gtgtcgtccg cctttttcga cagcggcttc atgtcttcgc cacccttgat
      481 cagcagccat tcgtcgccgc cgcgcaaacg cacaagatac cattcgccgc gcagcttttt
      541 tcctttgagc acgaagtgca gctttccgcc ttcgagttct ttcgagggat tctcgctcaa
      601 cggttcgaac gtgccgcggt cccacaccat cacggtgccg ccgccatatt gtccctgcgg
      661 aatcgtgcct tcgaaatcga tgtaggaaac cgggtgatct tcgacgtgca ccgcgagccg
      721 ccgctcgcct ttcgcatacg gcacgccctt cggcaccgcc cacgatttca acgtgccgtc
      781 gacctccagc cgaaaatcgt agtgcagccg cgacgccgcg tgtttctgaa tcacgaaacg
      841 atgcgccgcg ccacccgtga cgcgcccgcc tttcggttcg gccgttttct tgaaatcgcg
      901 ttttcggacg tattctttca gtgacatcgg cgcgcccgat gtccggcaaa cggacgcgct
      961 cgccagcgcg aatcacgccg ctcgcacgga tgcgggcgtg aacattgtca gcctcacccc
     1021 gccgctgttc cgcccgccac ggccagcccc ggcgtccgcc cgatacccgg acacgacgcg
     1081 ccatcgaacc acgtgccttc cacgaacgtc gtcgtcg
//
LOCUS       DASPLT010000311         1382 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_126245, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000311 DASPLT010000000
VERSION     DASPLT010000311.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1382)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1382)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1382
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_126245"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..339
                     /locus_tag="VEA63_03260"
     CDS             <1..339
                     /locus_tag="VEA63_03260"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HYD83035.1"
                     /translation="DYGEKLIEEGDHGNVFTSLGRNPGLARYVSETTSSENLLLMSGI
                     PSLKNREFSITYSRDGGRTWSARKAIDQLGWYSDLGVTRDKTIIAAYTVGFSEDLKIA
                     RFNLPWLLQP"
     gene            complement(546..>1382)
                     /locus_tag="VEA63_03265"
     CDS             complement(546..>1382)
                     /locus_tag="VEA63_03265"
                     /inference="COORDINATES: protein motif:HMM:NF024770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HYD83036.1"
                     /translation="DIGVICSEAVYRMPPRFIQVLDIFRALCAEEHLAVDVLEGQRFS
                     RTDPGRLMPRLVRSSPKACWVLVLADRRMQKWFESSGVPAVVYGNVYAGVSLPGAGIN
                     YHACIRHATSLLLAKGHRRIALVAYDARRAGEQDSVRGFREAFEAHRGEPAVPLVIER
                     PDSDADALCRQLDGVLRATRRPTAFVVTHTHHYATVATHLGMRGLRVPEDVSLICRSE
                     DPFLQFMRPQPVFYRANMEVAAKLLFDRVRHAMEGTAKPGDQRLIVPEIVTGGSVGPP
                     PA"
BASE COUNT          280 a          481 c          392 g          229 t
ORIGIN      
        1 gactacggcg agaaactcat cgaggaaggc gatcacggca acgtcttcac ctcgctcggc
       61 cgcaatcccg gcctcgcccg ctacgtcagc gagaccacca gcagcgaaaa tcttctgctc
      121 atgtctggca tccccagcct gaagaaccgc gagttcagca tcacttacag ccgcgacgga
      181 ggccgcacgt ggtccgcgcg caaagcgatc gaccaactcg gctggtattc cgatctcggc
      241 gtcacacgtg acaagacgat catcgccgcc tacaccgtgg gcttctccga agatctgaaa
      301 atcgcccgtt tcaatctccc gtggctgctg cagccgtgat cctgcgcgtg agccttatcc
      361 ggggaggcaa acctttgaaa ccacgaatgg acacgaatcc acacgaatga cgatccgggc
      421 aaaaagctgg tcgagggccg cacctcaagg tggtcaagtt ccgggcccga tccgcagata
      481 attccgctct gaattcgtgt gcattcgtgt ccattcgtgg ttcctgaaca ggtgcggcgc
      541 agcttttacg caggcggcgg tccgacgctt ccgccggtca cgatctccgg cacgatcaaa
      601 cgctgatcgc ccggcttcgc cgtgccttcc atcgcgtgac gcacccgatc gaacagcagc
      661 ttcgcggcga cttccatgtt cgcgcgataa aacaccggct gcggccgcat gaactgcaaa
      721 aacggatcct cgctgcgaca gatcagcgac acatcctccg gcacgcgcag cccgcgcatc
      781 ccgagatgcg tggccaccgt cgcgtagtga tgcgtgtgcg tgacgacaaa cgcggtcggt
      841 cgccgcgtcg cgcgtagcac gccgtcgagc tgccggcaga gcgcatccgc gtccgaatcg
      901 ggccgctcga tcaccagcgg caccgccggc tcgccgcgat gcgcttcaaa cgcctcgcga
      961 aacccgcgca cgctgtcctg ttcgcccgcg cgccgcgcat cgtaggcgac gagcgcgatg
     1021 cgccgatgtc ctttcgccaa cagcagcgac gtcgcatggc gaatgcatgc gtgatagttg
     1081 attcctgctc cgggcaacga aacgcccgcg taaacgttgc cgtaaaccac cgccggcacg
     1141 ccgctgctct cgaaccattt ttgcatccgg cgatccgcga gcacgagcac ccaacaagcc
     1201 ttggggctgc tgcgcacgag ccgcggcatc aagcgccccg gatccgtgcg actgaaacgc
     1261 tggccctcca acacgtcgac tgccagatgc tcctcggcgc agagcgcgcg aaaaatatcc
     1321 agcacctgga tgaagcgcgg cggcatgcga tacaccgctt cggaacagat cacaccgatg
     1381 tc
//
LOCUS       DASPLT010000312         1038 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_188092, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000312 DASPLT010000000
VERSION     DASPLT010000312.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1038)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1038)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1038
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_188092"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(69..926)
                     /gene="murI"
                     /locus_tag="VEA63_03270"
     CDS             complement(69..926)
                     /gene="murI"
                     /locus_tag="VEA63_03270"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012377019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HYD83037.1"
                     /translation="MIGVFDSGFGGLTVLASLLRALPHYDYLFLADSARAPYGARSPD
                     VINQFTLEAVEWLFDEGCTLVVLACNTASARALRNIQQLHLPVHRRDRRVLGVVRPSV
                     EALAALPPGAIPGETPPSDATGSVVVLGTESTIASDSYGIELRKLAPRLELIQQACPM
                     WVPLVEAGELSGPGAEYFLRQSLAPVLNRTPLPQRLLLGCTHYPLLLPTLRSLIPPSI
                     EILDQAEIVTTRLADWLQRHPEHETRLTRATTRRFATTDDPAWFATRGERLLGQSFTA
                     ERARLKQIA"
BASE COUNT          204 a          276 c          386 g          172 t
ORIGIN      
        1 atgatgtttg gccaagcggc ggcctggttt tggccggcta gaatgatttc acctctgaaa
       61 tatcacgctc aggcgatttg tttcaggcga gcgcgttcgg cggtgaagga ttgaccgagc
      121 aagcgttcgc cacgcgtggc gaaccacgcg gggtcatcgg tggtggcgaa gcggcgggtg
      181 gtggcacgcg tgaggcgtgt ctcgtgttcg ggatgacgtt gcagccagtc ggcgaggcgg
      241 gtggtgacga tctcggcttg gtcgagaatt tcgatgctcg gtggaatcag cgaacgaagc
      301 gtgggaagca gcagcgggta gtgggtgcaa ccgagcaaca gacgttgagg gagcggcgtg
      361 cgattcaaca cgggcgcgag cgactgacgc aggaaatact cggcgccggg gccgctcaac
      421 tcgccggctt cgacgagcgg cacccacatc gggcaggcct gctgaatgag ctcgagacgc
      481 ggtgcgagtt tgcgcagctc gatgccgtag gaatcggaag cgatcgtgct ttcggtgccg
      541 agaacgacga cggagcccgt cgcgtcggag ggcggcgttt cgccggggat ggcgccgggt
      601 ggcagtgcag cgagcgcttc tacggagggc cgaacgacgc cgagcacacg acggtcgcga
      661 cgatggacgg ggaggtgaag ttgctggatg ttgcgcagcg cgcgtgcgga ggcggtgttg
      721 caggcgagca cgacgagcgt gcaaccttcg tcgaaaagcc attcaaccgc ttcgagcgtg
      781 aactggttga tgacatccgg acttcgggcg ccgtaagggg cgcgcgcgct gtcggcgagg
      841 aaaaggtagt cgtagtgcgg gagcgcccgc agcagcgacg ccagcaccgt gagcccgccg
      901 aaacctgaat cgaacacgcc aatcatcggc ccgaggggaa agctccgggc gcatggaagc
      961 aagccaggtc cacgcatcgt gccattcgca catcggcgcg atggtcattc taaaagtgtc
     1021 acgtaatacg tgacactt
//
LOCUS       DASPLT010000313         2598 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_26200, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000313 DASPLT010000000
VERSION     DASPLT010000313.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 2598)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2598)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2598
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_26200"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..646
                     /locus_tag="VEA63_03275"
     CDS             <1..646
                     /locus_tag="VEA63_03275"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HYD83038.1"
                     /translation="IAREIAAAAVQRRHGVTGPFVLTVGDLQPRKNHIGLIRAFERLV
                     SEHPKLPHQLVIAGKDTWFADRVHSAARASTVSSRIRFTGWVSDEDLLNLYNSCDVMA
                     FPSFYEGFGLPILEAMACCRAVACSNSSAMPEVADSAALLFNPESRDDIVRALRDLLL
                     DPELRSRMERLGQARASLFTWERTARRTLEIYYDVAGAKAPAVATGPGVSASRR"
     gene            643..1326
                     /locus_tag="VEA63_03280"
     CDS             643..1326
                     /locus_tag="VEA63_03280"
                     /inference="COORDINATES: protein motif:HMM:NF022750.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HYD83039.1"
                     /translation="MKLLIFVLAASVAVAAPPDQPKSKSETLHFTVNWPSGLSLGEGQ
                     LISAQTPDGWSFSMNVEAAIPAFAVAESAKSSATADFCSVELEKTGTRGKREITETTT
                     FDASKMIATRETKKGGGKSEMRITACARDALTFLQFMRRELAAGKLPQAQPVYYGAPY
                     QTRVQYTGTTKIVANGEAIDADKLTATFKGPASEFAVDMWFARDAARTPVKAQIPVTV
                     GKFTVEFSR"
     gene            1329..>2598
                     /locus_tag="VEA63_03285"
     CDS             1329..>2598
                     /locus_tag="VEA63_03285"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HYD83040.1"
                     /translation="MRVAFFSPMPPSRSGIADYSAALLEPLKKRSKTDVFDSKSAVFD
                     PENYDIALYQVGNNGYHAFVYERAIETPGVVVMHESNLHHLIAEMTIKRNDWDGYLRE
                     VEFDGGPEALEYARRVRALDVGPDYEGVPMLRRILSRSRGVIVHSRFVEQDVRRAGYE
                     GPIAVIPHGAWIPEADRLAFRHRLAVDENTPLIGVFGFLKPYKRIAESLRAFKRLLRL
                     EPRAKMVLVGEPHPEFPLNRIIASLGLGANVRVLGFTPIEDFVGYIAACDIVLNLRYP
                     TVGESSGTLLRSLGLGKAVIVSDIGSFKEYPDEICLKVPVDSTEEDTLFEYLNLLVSR
                     PAVREAMGNRARDWVDRECNWDSVADRYVAFLEAVVQGREWNQSTTAAAAVGAQAPSA
                     PPPAAAIEPEYIFGWAADDDAKSYVRTHITR"
BASE COUNT          494 a          824 c          803 g          477 t
ORIGIN      
        1 cattgcgcgg gaaatcgcag ccgccgcggt tcagcgccgc cacggtgtca ccggcccgtt
       61 cgtcctcacg gtcggcgatc ttcagccgcg caagaaccat atcggcctga tccgcgcctt
      121 cgagagatta gtttcagagc atccgaaact gccccatcag ctcgtcatcg cgggaaagga
      181 cacctggttc gccgaccggg ttcattccgc cgctcgggcg agcactgttt ccagccgaat
      241 tcggttcacc ggatgggtgt ccgacgaaga tttactaaac ctctacaaca gctgtgacgt
      301 gatggcattc ccttcgttct atgagggatt cggcctgccg attctcgagg cgatggcttg
      361 ctgccgcgcg gtagcttgtt ccaacagttc cgcgatgccg gaagtggcag actcggctgc
      421 acttttattc aatcccgagt ctagggacga catcgtccgc gcgttgcgcg acctgctgct
      481 cgatccggaa cttcggtcgc ggatggaacg cctcggccag gctagggcgt cattgtttac
      541 gtgggaaagg actgcgcgtc gaacactcga gatctactac gacgtcgcgg gagcgaaagc
      601 tccggcggtc gcgaccggtc ccggcgtgtc cgcaagccgc cgatgaaatt actcattttc
      661 gtcctcgccg cctcggtcgc ggtcgctgca ccgccggacc agccgaagag taaatcagaa
      721 acgctccact tcaccgtgaa ctggccgagc ggcctcagcc tcggcgaagg tcagctgatc
      781 tctgcccaga ccccggacgg ctggagcttc tcgatgaatg tcgaagccgc catcccggca
      841 ttcgccgtgg ccgagtccgc gaagtcgagc gctacagcag acttctgttc cgtcgagctc
      901 gagaagacgg gcacgcgcgg aaagcgcgag atcaccgaaa cgaccacatt cgacgcttcg
      961 aagatgatcg ccacccgcga gacgaagaag ggcggcggca agagcgagat gcgcatcacc
     1021 gcctgcgcgc gcgatgctct cacgtttctg cagttcatgc ggcgcgaact cgcggccggc
     1081 aagctgccgc aggcgcagcc cgtctactac ggagcgccct atcagactcg cgtgcagtac
     1141 accggaacca ccaagatcgt cgcaaacgga gaggcgatcg acgcggacaa actgacggcc
     1201 acgttcaaag ggcctgcgtc cgagttcgct gtcgatatgt ggtttgcgcg cgatgcggcg
     1261 cggactccgg tgaaggcgca aatcccggtc acggtgggca agttcaccgt agagttctcc
     1321 cgctgacgat gcgggtcgcg ttcttctcgc ccatgccgcc gagccgcagc ggcatcgccg
     1381 actactccgc agcactgctg gaaccgctca aaaaacgctc aaaaacagac gttttcgact
     1441 caaaaagcgc tgttttcgac cctgaaaact acgacatcgc gctctaccag gtgggaaaca
     1501 acgggtatca cgccttcgtt tacgagcggg cgatcgagac acccggggtt gtcgtgatgc
     1561 acgaaagcaa cctgcatcac ctcatcgccg agatgaccat caagcgcaac gactgggacg
     1621 gatacctgcg cgaggtggag ttcgacggcg ggcccgaagc gctcgaatat gcccggagag
     1681 ttcgtgctct agacgttggc ccggactatg aaggcgtgcc catgctgcgc cgcatcctct
     1741 cacggtcgcg cggcgtgatc gtccacagcc gtttcgtcga gcaggacgta cgccgcgcgg
     1801 gctacgaagg tccaatcgca gtgatcccgc acggcgcgtg gattcccgaa gccgaccggc
     1861 ttgcctttcg tcaccggctt gccgtggacg agaacactcc gctcatcgga gtgttcgggt
     1921 ttctgaagcc gtacaagcgg atcgctgagt ctctgcgtgc tttcaagcgg ctgctgcgcc
     1981 tggagccgcg ggcgaagatg gtgctggtgg gggagccgca tcccgagttc ccgctgaacc
     2041 gcatcattgc gagtctcggg ctcggcgcta atgttcgcgt gctcggcttc actcccatcg
     2101 aggacttcgt cggatatatc gccgcgtgcg acatcgttct gaacctccgc tatccgaccg
     2161 tcggcgagag ttcggggacg ctgcttcgtt cgctcggtct cggcaaagcc gtgatcgtgt
     2221 ccgacatcgg ctcgttcaag gaataccccg acgagatctg tctcaaggtg ccggtcgatt
     2281 ctacggaaga agatacgctg ttcgagtatc tgaacctgct ggtgtcgcgt cccgcggttc
     2341 gcgaagcgat gggcaaccgc gcgcgcgact gggtggaccg cgagtgcaac tgggactcag
     2401 tcgcggaccg gtacgtcgcg ttcctcgaag cagtcgtgca aggcagggag tggaatcaat
     2461 caacaacggc ggcggccgcg gtcggcgcgc aagcaccttc ggcgccgccg cccgccgcgg
     2521 cgatcgaacc ggagtacatt ttcggctggg ctgcggacga cgacgcgaag agctacgtcc
     2581 gcacgcacat cacgcgcc
//
LOCUS       DASPLT010000314         1466 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_228551, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000314 DASPLT010000000
VERSION     DASPLT010000314.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1466)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1466)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1466
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_228551"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..290)
                     /locus_tag="VEA63_03290"
     CDS             complement(<1..290)
                     /locus_tag="VEA63_03290"
                     /inference="COORDINATES: protein motif:HMM:NF024586.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HYD83041.1"
                     /translation="MADANSSRQTSPANNSGFPKSPGDERYHMLLNSIDEGFCVIEML
                     FDASGRPCDYRFLEINPAFVRHSGLENAQGKRIRELAPAHEQHWFEIYGRVAL"
     gene            440..1243
                     /locus_tag="VEA63_03295"
     CDS             440..1243
                     /locus_tag="VEA63_03295"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - obsolete inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HYD83042.1"
                     /translation="MHLPVILEDVAAIARAAGEIIMRHFAAPIPTTAKSSRIDIVTAA
                     DTEAERFIVGELRTRFPEHHIVGEEGGGQGAAAATAPYHWFVDPIDGTVNFASKLPHF
                     CTSIALATPDRQPLLGAIYDPTRRELFTAVRGGGAQLNGEPLRVTQTAELIDAVITSG
                     FPYDKHTNPDNNLKEWSAFLMQIRGERRLGSAALDLCYVAAGRLDGYWEKDLKPYDAM
                     AGLLIVREAGGTVTDYKGAPNPQQADRGRYVASNGLIHDAMLRVLQSAQ"
BASE COUNT          283 a          435 c          487 g          261 t
ORIGIN      
        1 agcgcgacgc ggccataaat ttcgaaccaa tgttgctcgt gcgccggcgc cagctcgcgg
       61 atgcgtttgc cttgcgcgtt ttcgagaccg ctatggcgga cgaacgcggg attgatttcc
      121 agaaaccggt aatcgcaagg ccggccggaa gcatcgaaca gcatctcgat gacgcagaag
      181 ccctcgtcga tcgagttcag cagcatgtgg tagcgctcgt caccgggcga cttgggaaaa
      241 cctgaattgt tcgcgggcga ggtctgtcgg gaagaatttg cgtcagccat gttggtcagc
      301 cagtccgcac gcagcgggat aggagcgcgc ttggacccgc gcgaggcgaa cgtgagccct
      361 gcgcgagggg aaaccacata aaacttcggg gctcacgtgg cggattgcgt ggaagcagag
      421 cgtccccgac tgtggcgcca tgcacctgcc tgtaatcctc gaagacgtcg ccgcgatcgc
      481 gcgtgctgcg ggtgagatca tcatgcgcca ctttgcggca cctattccaa cgacggcgaa
      541 gagcagtcgc atcgatatcg tgaccgcggc ggatacggaa gcggaacgct tcatcgtcgg
      601 cgaattgcgg acgcggtttc cggagcatca catcgtgggc gaggaaggcg gcggacaagg
      661 ggcggcggcc gcaaccgcgc cgtatcattg gtttgtggat ccgatcgacg gcacggtgaa
      721 cttcgccagc aagctcccgc atttttgcac gagcatcgcg ctcgccacgc cggaccggca
      781 gccgttgctc ggcgcgatct acgatccgac gcgacgcgag ttgttcacgg cggtgcgcgg
      841 cggcggcgcg cagctcaacg gcgaaccgtt gcgcgtgaca caaaccgctg agctgatcga
      901 tgcggtgatc acgagtggtt ttccttacga caagcacacg aatcccgaca acaacctgaa
      961 ggagtggtcg gccttcctga tgcaaattcg cggcgagcgt cgtctcggtt ccgcggcgct
     1021 tgatctgtgt tacgtcgccg ccggccggct cgacggttac tgggagaagg acttgaaacc
     1081 ctacgacgcg atggccgggc tgttgatcgt ccgcgaagcc ggcggaacgg tgaccgacta
     1141 caagggcgcg ccgaatccgc agcaagccga ccgcgggcgt tacgttgcga gcaacgggct
     1201 gattcacgac gccatgctgc gcgtgctgca atcggcgcag tgagctgggc tcgctgcgac
     1261 tgctccgcgg ccttcgtcga acacggccct cgaccggcct tgtcatgcgc gtgatcgtgg
     1321 cgcctactgc gcgttcatgc ctgcggaatt cacggacgaa gaagaagagt tgttcaacga
     1381 gatcggtgcg tcggtgatga agcggatgcg caaggcgcga aatcccggcg atctgctttc
     1441 gattctgcgc acggcgcaca aacagt
//
LOCUS       DASPLT010000315         1587 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_86176, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000315 DASPLT010000000
VERSION     DASPLT010000315.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1587)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1587)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1587
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_86176"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            405..>1587
                     /locus_tag="VEA63_03300"
     CDS             405..>1587
                     /locus_tag="VEA63_03300"
                     /inference="COORDINATES: protein motif:HMM:NF014763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HYD83043.1"
                     /translation="MASRYLSFLHRSIQGRLALLVGAVLLPAAVLVAWLIFKGYENER
                     RQLEHHLIAAVRATSLLVDSEINERESLLFGLATSGRLQRGDLGLFRAQAQAVVRRSD
                     EWILLLDAAGNQRMNTLGPEDVDLAGFAYDPEFRQTVEAGRTYISNLVPGTGEDPPLL
                     FVGIPIFQEQKLLYTLVLVTRPAVFRDALVRGGVSPAWTVSIVDRQGVLAARTRNPVD
                     YVGAKAGDLIADAITKSQEGVLESVTLDGISSITAHSRSLKSGWTLIVAAPAQELFAS
                     AQRLLGVALAVSLLLGAGGAMLAVWVGRGVVSAVQALVQETEAMGRGEAVAGESTGIA
                     ETDTVLRALAETSARLAGREAELKQLNESLTRAGRAKDEFLAALSHELRTPLNPVLLT
                     AS"
BASE COUNT          245 a          486 c          528 g          328 t
ORIGIN      
        1 gaacgcatct gagaaaaacc agccgtttca tatctggctt cgcggcgttg cggcaccatt
       61 gacgagcccc gctcgttcga tcctcaccat cctgctgccc tccacgctgg cggaatgatc
      121 tcattcccat ggtgtctcgc cgccgccgcg cagagagcgg cctttagcat gaccttccga
      181 cgaggtttcg agatttacac cacgaccacg gagctcgcaa agagcagttt gggagcgtct
      241 tcgcgctctt tctgagcctc gggtggaatc gttttgatcc gagatatcgg tgcaaaatcc
      301 tgttttccat acagccgtcg gagcacggtg aacggttttg cagatgcgct tcggatgcgg
      361 cctcttgact cgtttccggg cgtcgcctcg gatggcgcga cttcgtggcg agccgttacc
      421 tgtctttcct tcatcgctcc attcaaggcc ggctcgccct gctggtcggc gcggtgctgc
      481 tgccggcggc cgtgctggtg gcttggctga tcttcaaggg ttacgaaaac gagcgccgcc
      541 agctcgagca tcacctgatc gcagcggtcc gcgcgacttc gctgctggtc gattccgaaa
      601 tcaatgaacg ggagtcgttg ctgttcggcc tcgcgacgtc cggtcggctc cagcgcggcg
      661 atctcgggtt gtttcgcgcc caggcgcagg ccgtggttcg gcgatcggac gagtggattc
      721 tgctcctcga tgcggcggga aatcagcgga tgaacacgct ggggccggag gacgtggacc
      781 tggccggctt cgcttatgac ccggagttca ggcagaccgt ggaggccggt cgcacttaca
      841 tttccaatct cgtgccaggc accggcgagg acccgccgct gttgttcgtt ggcatcccga
      901 tttttcagga gcagaagctc ctttatacac tcgtgctggt tacgcgtcct gcggtctttc
      961 gcgacgcgct tgtgcgcggc ggtgtgagtc ccgcgtggac ggtttccatc gtggatcgtc
     1021 aaggcgtgct cgcggcacgc acgcgcaacc ccgtggacta cgtgggtgcc aaagcgggcg
     1081 acttgatcgc cgacgccatc acgaagagtc aggaaggggt ccttgaaagc gtcacgctcg
     1141 atggcatctc gagcatcacc gcgcattctc ggtcgttgaa atcaggctgg acccttatcg
     1201 tcgcggcgcc ggcgcaggaa ttattcgctt cagcgcagcg gttgctcggc gtggcgctgg
     1261 ccgtatcact tctccttggc gccggtggcg cgatgctcgc ggtgtgggtg gggcgcgggg
     1321 tcgtatcggc ggtgcaggcg ctggtccagg aaacggaggc gatgggccgc ggcgaagcgg
     1381 tcgcgggcga atcgaccggc atcgctgaaa cggataccgt gctgcgcgcg ctcgcggaga
     1441 cctcggcgcg actcgcgggg cgcgaggccg agctgaagca gctcaatgaa tcgctcaccc
     1501 gcgcgggccg cgcgaaggac gagtttctcg cggcgctctc gcacgagttg cgcacgccgc
     1561 tgaacccggt gctgttgact gcgagcg
//
LOCUS       DASPLT010000316         2853 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_209079, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000316 DASPLT010000000
VERSION     DASPLT010000316.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 2853)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2853)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2853
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_209079"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2337
                     /locus_tag="VEA63_03305"
     CDS             <1..2337
                     /locus_tag="VEA63_03305"
                     /inference="COORDINATES: protein motif:HMM:NF019113.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HYD83044.1"
                     /translation="YGAKTAESFVQPDGSIATYKMEDYNIDMIPPGKVLLYRWEKGVR
                     DEKFKIAIETLREQLHKQPRISEGGFWHKLRYPYQMWLDGLFMGSPFLAHYGKVFEEP
                     AAFDDVAKQITLMDKHGYDPKTGLYYHAYDEKRAQPWANKETGQSPNFWGRAVGWWAM
                     SLVDSLDFFSPTHPEVEHINEILRRTADGIVRWQDPKSGLWWQVMDQSDREGNYLEAT
                     SSSMFVYALAKGVNRGYLPREKYLPTILKAYEGLIRDLVRTEADGKVTLTQCCEVAGL
                     GYTTSKGRPRDGSFDYYISEPIIDNDPKGVGPFIMAGIEMEQLLANTNSSGSAREPGD
                     LGSRGWADYEAVLARIKAPTFPDRDFPITDFGAKPGADASAAIRAAIDACHKAGGGRV
                     VVPPGAWGSGAIHLKSNVNLHVSEGATINFSTNPADFPIVFTRWEGVELMNYSPLIYA
                     FEQQNIAVTGKGTLDGQASLENWWSWNRKGERPQKQKAARDRLIALGETDTPVAERVF
                     GDGSFLRPNFIQPYRCQNVLIEGVTILRSPMWIIHPVLSQNITVRGVKIITHGSNNDG
                     CDPESSRDILIEDCVFDTGDDCIAIKSGRNGDGRRLNVPSENIVIRNCVMKDGHGGVV
                     LGSECTGGIRNIFVENCTMDSPDLDRGLRFKNNAVRGGVLENVFMRNVKIGRVGEAVL
                     TIDLLYEEGAKGDFKPVVRNVQLDHITSSASPRVMFIRGFPGAVIENIRISNSMFNSV
                     TETEVVRHAGTISFDNVTITPAKAARSLNSVLPPSASK"
     gene            2462..>2853
                     /locus_tag="VEA63_03310"
     CDS             2462..>2853
                     /locus_tag="VEA63_03310"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HYD83045.1"
                     /translation="MSVPAPLPTPPLRTAHARWIVCAMLLMAATINYIDRQVIGILKP
                     TLTEQFGWADERIYAGIVFSFTFAYAIGMLIAGRVIDKIGLRLGFSIAIVVWSLAAVG
                     HAFAHKLPDFILPTLVIDPNIGLSFITLT"
BASE COUNT          567 a          987 c          784 g          515 t
ORIGIN      
        1 tacggcgcga agaccgccga gtcgttcgtg cagcccgacg gctccatcgc gacctacaag
       61 atggaggact acaacatcga catgatcccg cccggcaagg tgctgctcta tcgctgggaa
      121 aagggcgtgc gcgacgagaa gttcaagatt gcgatcgaga ccttgcgcga gcaactccac
      181 aagcagccgc gcatcagcga aggcggcttc tggcacaagc tgcgttatcc gtatcaaatg
      241 tggctcgacg gcctcttcat gggctcgccg tttctcgcgc actacgggaa agttttcgag
      301 gagcccgccg cgttcgacga cgtcgcgaag cagatcacgc tgatggacaa acacggctac
      361 gatcccaaga ccggccttta ctatcacgct tacgacgaga aacgagccca gccttgggcg
      421 aacaaggaaa ccggccagtc cccgaatttc tggggccgcg ccgtcggctg gtgggcgatg
      481 tcgctcgtcg actcgctcga tttcttctct ccgacgcacc ccgaggtgga gcacatcaac
      541 gaaatcctcc gccgcaccgc cgacggcatt gttcgctggc aggatccgaa gtccggcctc
      601 tggtggcagg tgatggatca gagcgatcgc gagggcaact atctcgaagc cacctcttcc
      661 agcatgttcg tctacgcgct cgcgaagggc gtgaaccgcg gctatctccc gcgcgaaaaa
      721 tatctcccca caatcctcaa ggcctacgaa ggcctcatcc gcgatctcgt ccgcaccgaa
      781 gccgacggca aggtcacgct cacccaatgc tgcgaagtcg ccggcctcgg ttatacgacc
      841 tcgaagggcc ggccgcgcga cgggtcgttc gactactaca tcagcgaacc catcatcgac
      901 aacgacccga agggcgtcgg cccgttcatc atggccggca tcgaaatgga acagttgctc
      961 gccaacacga attccagtgg ttcggcgcgc gagcccggcg atctcggctc ccgcggctgg
     1021 gccgattacg aagccgtgct cgcgcgcatc aaggcgccga cgtttcccga ccgcgatttt
     1081 ccgatcaccg atttcggcgc aaaaccgggt gcagatgcct ccgccgcgat tcgcgccgcg
     1141 atcgacgcct gtcacaaagc cggcggcggc cgcgtcgtcg tcccaccggg cgcttggggc
     1201 agcggcgcca ttcacttgaa gagcaacgtc aacctccacg tcagcgaggg cgccaccatc
     1261 aatttctcca ccaaccccgc cgacttcccg attgtcttca cccgttggga aggcgtcgag
     1321 ttgatgaact actcgccact gatctacgcg ttcgagcagc agaacatcgc cgtcaccggc
     1381 aagggcaccc tcgacggcca ggcgtcgttg gaaaactggt ggagctggaa ccgcaaaggc
     1441 gagcgtccgc agaaacagaa ggccgcccgc gaccgcctca tcgccctcgg cgaaaccgac
     1501 acgcccgtcg ccgaacgcgt tttcggcgac ggcagttttc tccgccccaa cttcatccag
     1561 ccttatcgct gccagaacgt tctcattgaa ggcgtgacca tcctgcgttc gccgatgtgg
     1621 atcattcatc ctgtgctgtc gcaaaacatc accgtgcgcg gcgtgaagat catcacgcac
     1681 ggttcgaata acgatggctg cgatcccgaa tcgtcgcgcg acatcctgat cgaggactgc
     1741 gttttcgaca ccggcgacga ctgcatcgcc ataaaatccg gccgcaacgg cgatggccgc
     1801 cggctcaacg tgccgagcga gaacatcgtc atccgcaatt gcgtcatgaa agacggccac
     1861 ggcggcgtcg ttctcggcag cgagtgcacc ggcggcatcc gcaatatttt cgtcgagaac
     1921 tgcacgatgg actcgcccga tctcgaccgc ggcctccgct tcaagaacaa cgccgtgcgc
     1981 ggcggcgttc tcgaaaacgt tttcatgcgc aacgtgaaga tcggccgcgt cggcgaagcc
     2041 gtgctcacca tcgacctgct ctacgaggaa ggcgcgaagg gcgatttcaa gccggtcgtg
     2101 cgcaacgtgc aactcgacca catcaccagc tccgccagtc cgcgcgtgat gtttatccgt
     2161 ggttttccgg gcgcggtgat tgaaaacatt cgcatcagca actcgatgtt caacagtgtg
     2221 actgaaaccg aagtcgtgcg gcacgccggc accatcagct tcgataacgt caccatcacg
     2281 cctgccaagg cggcccgcag cctcaactcc gtcctgccac cgtccgccag caagtaacgc
     2341 ccgccaacaa cgccgagagg gcgcgagtcc cctcccggcc ggccagttca gcctcgcttt
     2401 gcttttcggc cccgcggaaa acccctcttc tcttttcttc acctcaccaa ccccgatcga
     2461 aatgtccgtt cccgctccac ttcccacccc gccgctccgc accgctcacg cccgctggat
     2521 tgtctgcgcc atgctgctga tggcggccac gatcaattac atcgaccggc aggtgatcgg
     2581 catcctgaaa cccacgctca ccgagcaatt cggctgggcc gacgaacgca tctacgccgg
     2641 tatcgtcttc agtttcacgt ttgcctacgc gatcggcatg ctgatcgccg gccgggtgat
     2701 cgacaaaatc ggcctccgcc tcgggttctc catcgcgatt gtggtctgga gcctcgccgc
     2761 cgtcggccac gcgttcgcgc ataaactgcc ggacttcatc ctcccgacgc tcgtcatcga
     2821 tccgaacatc gggctctcgt tcatcacgct cac
//
LOCUS       DASPLT010000317         4251 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_291394, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000317 DASPLT010000000
VERSION     DASPLT010000317.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 4251)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4251)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4251
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_291394"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..893
                     /locus_tag="VEA63_03315"
     CDS             <1..893
                     /locus_tag="VEA63_03315"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HYD83046.1"
                     /translation="SAGGWEKRRRFSFGSVFAAVSSRTERIVDADAQRCQGRGLRKGG
                     PVNRISRTVAGLLIANIVVFAAGYLLPNANDSLVAAGALWYPANSHFGLWQVVSYMFL
                     HGSLGHIFFNMFALVSFGSVLEREWGAARFLIFYFLCGVGAGLIQTAINWQEFHGLHD
                     LLVGSGLAPSAITSLLETGSGVLPADPAIKAVLIELYGIYATPTVGASGAIYGLLVAF
                     GCLHPNAKLALMFLPFPIAAKFFIPGLLLLDLFSGVTGFSLFGASIAHFAHLGGAAIG
                     FLLMLLWRKRSEPDAATLRT"
     gene            complement(998..>4251)
                     /locus_tag="VEA63_03320"
     CDS             complement(998..>4251)
                     /locus_tag="VEA63_03320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007362155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase subunit
                     alpha"
                     /protein_id="HYD83047.1"
                     /translation="PHGEERFVLAEVADTLGKALTNVCLARGETDIFNDTNRAWIIRI
                     CREVAANLHELGRVSSPVRLSLDALYELIEKTLVDNNAYFVAKSLLLNRARKISINRE
                     AAAVSTLRVIRRNNQVVPWSDQKVEIAVRKTFLSLQRDSAPAVAITKAVAARIHASKQ
                     SFVHIEEIQDMVQEELMKTGHYKVAEAYILFRAERAIARDAGNADGLAVSTETAVDAS
                     PSTVTPGQASMIVVKKADGTNVFWDGADLRKRIEFARIGLELCLSNDEIEVELRRSVY
                     DQISQKDLDATIILNSKTLIEKDADFAKFAGRIQLTFLYEEILGWDIMADGIGALKAA
                     HQRAFRKYLEHGVAIKRLNPRLLDYDLTRLAGALDPSSDLEFDFLGIQTMYERYLIID
                     KTKRPNRRIETPQFFWMRVSMGLFLDEKGDREEKVTSLYELYKSRRFCSSTPTLFNSG
                     TLHSQLSSCYLYYVDDSIEGIMLRGIAENAFLSKWAGGLGGSWTAVRGTGAYIGGTNG
                     ESQGVIPFLKLHNDQLVAVNQGGKRKGSGCAYLETWHNDIFEFLELRKNTGDDRRRTH
                     DMNTANWIPDLFMKRMEARQSWTLFRSNQVPDLHELYGKKFEEAYLRYEKLADEGKIH
                     GQKVEALELWKKMLSMLFETGHPWITFKDACNLRSPQDHVGVIHSSNLCTEITLNTSN
                     DETAVCNLGSVILETHLLPNGEIDHKKLRETIRTAVRALDNVIDINFYPTKAAETSNR
                     RHRPIGLGVMGLAHSLYLRGHAFASSEAVEFNDEAMEAIAYYAYEASSDLAAERGTYS
                     SYKGSKWDRGLLPQDTLDLLEQERGVEVEVPRGGRMDWTPVREKIAKHGMRNSNVLAI
                     APTATISNITATSPCIEPTYKNLFVKSNLSGEFIVLNPFLVKDLKARGLWDQDMIDNL
                     KYFDGELKDIERIPADLKARYLTAFDIDAKWIIDAAARRQKWIDQSQSVNLWIKTPDL
                     KTLSHMYRQAWHVGLKTTYYLRSLGASNIEKATVGVKKEMRGAVSEGSSGGQNSGDTG
                     GTHSVAAMALGADASGSQLSALSSQSKKTYTEAEKNACSIEAMRNGEICEACQ"
BASE COUNT          692 a         1191 c         1432 g          936 t
ORIGIN      
        1 ggagcgctgg cggatgggaa aagagaagaa ggttctcctt cggctcggtc ttcgcggcgg
       61 tgagttcgcg cacggagcgg attgtcgacg ccgacgcgca gcgttgccaa gggcgggggc
      121 ttcgcaaggg tggccctgtg aatcgtatct cccggacggt cgccggtctg ctgattgcca
      181 acatcgtggt gtttgccgcg ggctacctgc tgcccaacgc gaacgactcg ttggtcgcgg
      241 ccggcgctct ctggtatccg gcgaacagcc actttggtct ctggcaggtg gtctcgtata
      301 tgttcctgca cgggagcctg ggccatatct tcttcaacat gttcgcgctt gtttccttcg
      361 gctcagtgct ggaacgggaa tggggcgcgg cgcgcttttt gattttctat tttctttgcg
      421 gcgtgggcgc cgggctgatc cagacggcga tcaactggca ggaattccat ggccttcatg
      481 atctcttggt cggctcaggg ttggcgcctt ccgcgatcac ttccctgctg gagactggca
      541 gcggcgtgct gcctgcggat ccggcaatca aggcggtgct gatcgagctc tacgggatct
      601 acgcgactcc aacggtcggc gcgtccgggg cgatttacgg cctcctggtc gcctttggat
      661 gtctccatcc caacgccaag ctcgccctga tgtttctgcc gttccccatc gccgcgaaat
      721 tcttcatccc gggactgctg ctcctcgatc tgttttccgg ggtcaccggc ttttcgcttt
      781 tcggcgcgag cattgcccat tttgcgcacc ttggtggagc cgcgatcggc ttcctcctca
      841 tgctgctatg gcgcaaacgt agcgagcccg acgccgccac acttagaacg tagtcgcagc
      901 gatgcgccgc attccgacgc acgtggcgag cataacgggt cacggcgtga cgcccccggc
      961 gggccggccc tacatggtag ggacggccgc ccacacctta ctggcaggct tcgcagattt
     1021 cgccgttgcg catcgcttcg atgctgcagg cgttcttctc ggcttcggtg taggtttttt
     1081 ttgattgaga gctgagggct gagagctgag agcccgatgc atctgcgccc aaggccatcg
     1141 cggccacact gtgcgtgccg ccggtgtcgc cgctgttttg accaccgctc gagccttcgc
     1201 tcacggcgcc gcgcatttct tttttcacgc cgacggtcgc cttctcgata ttcgaggcac
     1261 cgaggctgcg gaggtaatag gtggttttca ggccgacgtg ccaggcttgg cgatacatgt
     1321 ggctcagggt tttgaggtcc ggagttttga tccacaggtt tacggactgg ctttggtcga
     1381 tccacttctg acgacgcgcg gcggcgtcga tgatccactt ggcgtcgatg tcgaaggcgg
     1441 tcaggtaacg cgccttcagg tccgcgggga tgcgctcgat gtccttcagc tcgccgtcga
     1501 aatacttcag gttgtcgatc atgtcctggt cccacaggcc gcgggctttc aggtccttca
     1561 cgaggaaggg attcaggacg atgaactcgc cggacaggtt cgatttaacg aagaggttct
     1621 tatacgtcgg ctcgatgcag gggctcgtcg cggtgatgtt cgagatcgtc gcggtcggcg
     1681 cgatcgcgag gacgttgctg ttgcgcatgc cgtgcttcgc aatcttttcg cggacaggcg
     1741 tccaatccat ccggccgcca cgcggcactt cgacttcgac gccgcgttcc tgctcgagga
     1801 ggtcgagcgt gtcctgcggg agaagtcccc tatcccactt cgaacctttg taggacgaat
     1861 acgtgccgcg ctcggcggcg aggtcgctcg acgcttcgta ggcgtagtaa gcgatcgctt
     1921 ccatcgcctc gtcgttgaac tcgaccgcct cggacgacgc gaacgcgtga ccgcgcaggt
     1981 agagcgaatg cgcgagaccc atcacaccga gaccgatcgg acggtggcgg cggttggacg
     2041 tctcggcggc cttcgtcggg tagaaattaa tgtcgataac gttgtcgagc gcgcggaccg
     2101 ccgtgcggat cgtctcgcga agttttttgt ggtcgatctc gccgttgggg aggagatgcg
     2161 tctcgaggat gaccgagccg agattgcaga cggcggtctc gtcgttggac gtgttgagcg
     2221 tgatctcggt gcagaggttc gacgagtgga tgacgccaac gtggtcctgc ggggagcgca
     2281 gattgcaggc gtccttgaac gtgatccacg ggtggccggt ctcgaaaagc atcgagagca
     2341 ttttcttcca gagctcgagt gcttcgactt tctggccgtg aattttgcct tcgtcggcga
     2401 gcttctcgta acggaggtag gcctcctcga atttcttacc gtagagctcg tggagatccg
     2461 gcacctggtt cgagcggaag agcgtccagg actggcgggc ttccatacgc ttcatgaaca
     2521 ggtcgggaat ccagttcgcc gtgttcatgt cgtgcgtgcg gcgacggtcg tcgccggtgt
     2581 ttttccgcag ttcgaggaat tcgaagatgt cgttgtgcca cgtttccaga tacgcgcagc
     2641 cagagccctt gcgtttgccg ccctggttga cggcgacgag ctggtcgttg tgcaacttca
     2701 ggaacgggat gacgccctgc gattcgccgt tggtgccgcc gatgtaggcg ccggtgccgc
     2761 ggaccgcggt ccacgaaccg ccgaggccgc ccgcccactt cgaaaggaag gcgttttcgg
     2821 cgatgccgcg gagcatgatg ccctcgatcg aatcgtcgac gtaatagaga tagcaggacg
     2881 agagctgcga gtggagcgtg ccggagttaa agagcgtcgg cgtcgaggag cagaaacggc
     2941 ggctcttgta gagctcgtag agtgacgtga ccttttcttc gcggtcgcct ttttcgtcga
     3001 ggaagaggcc catcgacacg cgcatccaga agaactgcgg cgtctcgatg cggcggttcg
     3061 gcctcttggt tttgtcgatg atcaggtagc gctcatacat cgtctggatg ccaaggaaat
     3121 cgaactcaag gtcggacgac ggatcgagcg cgccggcgag acgcgtgaga tcgtaatcga
     3181 ggaggcgcgg gttgaggcgt ttgatcgcga cgccgtgctc gagatatttg cgaaacgcgc
     3241 gttggtgcgc ggctttcagc gcgccgatgc cgtcggccat gatgtcccaa ccgaggatct
     3301 cctcgtagag gaacgtgagc tggatgcggc cggcgaattt cgcgaagtcg gcgtccttct
     3361 cgatcagcgt tttcgagttg aggatgatcg tcgcgtcgag gtccttttgc gaaatctggt
     3421 cgtaaacgga gcggcggagt tcgacttcga tctcgtcgtt ggaaaggcag agctcgaggc
     3481 cgatgcgggc gaactcgatg cgcttgcgga ggtcggcgcc gtcccagaat acgttcgtgc
     3541 cgtcggcttt tttgacgacg atcatcgagg cctggccggg cgtgacggtg gacggcgaag
     3601 cgtcgaccgc ggtctcggtg gagaccgcga gaccgtcggc gttgccggcg tcgcgggcga
     3661 tcgcgcgttc ggcgcggaaa aggatgtagg cttcagcgac cttgtagtgg ccggtcttca
     3721 tcaactcttc ctgcaccatg tcctgaattt cctcgatgtg gacgaaggat tgtttcgacg
     3781 cgtggatgcg cgcggcgacg gccttggtga tcgccacggc gggcgcggaa tcgcgctgga
     3841 gcgagaggaa ggttttgcgg acggcgatct cgaccttctg gtcgctccac ggcacgacct
     3901 ggttgttgcg gcggatgacg cggagcgtgg agacggcggc ggcctcgcgg ttgatggaga
     3961 tcttgcgagc gcgattgaga agaaggctct tggcgacgaa gtaggcgttg ttgtcgacga
     4021 gcgttttttc gatgagctcg taaagcgcgt cgagcgagag gcggacgggt gaggaaacgc
     4081 ggccgagctc gtggagattg gcggcgactt cgcggcagat acgaatgatc caagcgcggt
     4141 tcgtgtcgtt gaaaatatcc gtctcgccgc gcgcgaggca gacgttggtc agcgccttgc
     4201 cgagcgtgtc ggcgacttca gcgaggacga aacgttcttc gccgtgcggg c
//
LOCUS       DASPLT010000318         1622 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_291397, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000318 DASPLT010000000
VERSION     DASPLT010000318.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1622)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1622)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1622
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_291397"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1622
                     /gene="topA"
                     /locus_tag="VEA63_03325"
     CDS             <1..>1622
                     /gene="topA"
                     /locus_tag="VEA63_03325"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HYD83048.1"
                     /translation="MGINPDKGWQARYQILPGKEKVVSELKKVAASADHIYLATDLDR
                     EGEAIAWHLQEAIGGDPKRYSRVVFNEITKKAIEAAFAQPGELDMKHVEAQQARRFLD
                     RVVGFMVSPLLWAKVARGLSAGRVQSVAVRLIVEREREIRAFVPEEYWELYAQLLAAK
                     QKDALRFQVIRQNGKEFRPGNEALTQAAVKVLQGATYSVASREDKPTTSKPKAPFITS
                     TLQQAASTRLGFSVKKTMMMAQRLYEGGFITYMRTDSTHLSADAVAACREFIEDKYGK
                     KYLPEKPNSYSSREGAQEAHEAIRPSDVNVSENSSVLSAMERDAVRLYNLIWQQFVAC
                     QMPPARYKSTTIDVAAADFELRIKGRVVEFDGYTRVFPANEKDDDVVLPDLKPGDTLK
                     LDKLEPMQHFTKPPARYSEASLVKELEKRSIGRPSTYAAIISTIQERGYVKLENRRFY
                     ALKMGDIVTERLVENFKDLMDYDFTANMENRLDDIAQGNIDWIKVLDEFYANFTEQLA
                     KAQAEDGGMRNNDPTETTIKCPVCGRNMQIRTG"
BASE COUNT          384 a          491 c          489 g          258 t
ORIGIN      
        1 atgggcatca acccggacaa gggttggcag gcccgttacc agatcctgcc cggcaaggaa
       61 aaagtcgtca gcgagttgaa gaaggttgcc gccagcgccg accacatcta cctcgcaacc
      121 gaccttgacc gcgaagggga agccatcgcg tggcatttgc aggaagcgat cggtggcgat
      181 ccgaaacgct acagccgcgt cgtgttcaac gagatcacga agaaggcaat cgaagctgcg
      241 tttgcgcagc cgggcgaact cgacatgaaa cacgtcgagg cacagcaggc gcggcgcttt
      301 cttgaccgcg tcgtcggttt catggtgtcg ccgcttttgt gggcaaaggt tgcgcgcggt
      361 ctctctgccg gtcgcgtgca gtcggtggcc gtgcggctga tcgtcgaacg tgagcgcgaa
      421 attcgcgcgt tcgtgcccga agaatactgg gaactctacg cgcagttgct cgccgcgaag
      481 cagaaagacg cgttgcgctt ccaggtgatc cggcagaacg gcaaggaatt ccggccaggc
      541 aacgaagcgc tgacgcaggc cgccgtcaaa gtattgcagg gtgcgacgta tagcgtcgca
      601 tcgcgcgaag acaagccgac cacatcgaaa ccgaaagcgc cgttcatcac gtccacactg
      661 cagcaggccg catcgacgcg cctcggcttc agcgtgaaga aaacgatgat gatggcgcag
      721 cgtctgtatg aaggcggctt catcacgtac atgcgtaccg attcgacgca cctcagcgcc
      781 gatgccgttg ccgcgtgccg cgagttcatc gaagacaagt acggcaagaa atacctgccc
      841 gaaaagccga acagctacag cagccgcgaa ggcgcgcagg aagcgcacga ggcgattcgc
      901 ccatccgacg tgaacgtaag cgagaattcg tcggtactga gcgcgatgga acgcgacgcg
      961 gtgcgcctgt acaacctgat ctggcagcag ttcgtcgcct gccagatgcc gccggcgcgt
     1021 tacaagagca cgaccatcga cgtcgccgcc gcggatttcg aactgcgcat caagggccgc
     1081 gtcgtcgaat tcgacggtta cacgcgcgtg tttcccgcga acgagaaaga cgacgacgtc
     1141 gtgctgccag atctcaagcc cggcgacacg ctgaagctcg acaagctcga accgatgcaa
     1201 catttcacga agccgccggc gcgttacagc gaagcgagcc tcgtgaagga actcgagaaa
     1261 cgcagcatcg gccgtccgtc cacgtacgcg gcgatcattt cgacgatcca ggaacgcggt
     1321 tacgtcaaac tcgagaaccg gcgcttctat gcgctgaaga tgggcgacat cgtcaccgaa
     1381 cgcctcgtcg agaatttcaa ggacctgatg gactacgatt tcacggctaa catggaaaac
     1441 cgcctcgacg acatcgcgca gggcaacatc gactggatca aggtgctcga cgagttttac
     1501 gcaaacttta ccgagcagct cgccaaagcc caggccgaag acggcggcat gcgcaacaac
     1561 gatcccaccg aaacgacgat caaatgcccg gtgtgcggcc gcaacatgca gattcgcacc
     1621 gg
//
LOCUS       DASPLT010000319         1359 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_188124, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000319 DASPLT010000000
VERSION     DASPLT010000319.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1359)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1359)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1359
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_188124"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            177..452
                     /locus_tag="VEA63_03330"
     CDS             177..452
                     /locus_tag="VEA63_03330"
                     /inference="COORDINATES: protein motif:HMM:NF012759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HYD83049.1"
                     /translation="MSDSLHARLKRLIVDTLKLHDLQPEDIRDDEPLIGSGLNLDSID
                     ALELVVSLEKEFGIKITSSEESRTALASVTQLAEFIRAHAPQDRLPA"
     gene            494..>1359
                     /locus_tag="VEA63_03335"
     CDS             494..>1359
                     /locus_tag="VEA63_03335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD83050.1"
                     /translation="MIASHTLQPVAIEAAGCITALGDGPATWAALLRNEVALQERPVL
                     GPDGGDAVPLALVPGRSLEEAAPPAWAGLTEPLVRAIPEDRWGSARRPVFVTSSNFGV
                     GSLYAYQRSKDTAHLAYGTPFATVEFLRRRFNWDQNVAVLSHACVSAHLGLLLAARTL
                     AAGLADRALVFSFDFVSPFVAGGFHSLKILNGGFPAPYSARATGSIGLGDGAAFAVLT
                     RDPGPSSTGLRLEAQALHNEMHHFTANRADGSGFRACLAPVRDAVGSRRIWVKGHGTG
                     TLEAGRLEAETLA"
BASE COUNT          216 a          523 c          376 g          244 t
ORIGIN      
        1 atgatgttgt atttctggga aacgatggat gcctgcgtgc gccccgctgc cgtgctcgat
       61 gccttgcgcg ccgccggctt cggccaggtc tcccgcaccc cgcaactcgg cctcttcagc
      121 gaatattccg ccacgaaacc ctaaaccttc gtccagcgct cccgcgcgtt tttcgcatgt
      181 ccgattcgct ccacgcccgc ctcaaacgcc tcatcgtcga cacgctcaag ctgcacgatc
      241 tgcagcccga agatattcgc gatgacgaac cattgatcgg ttccggactc aacctcgact
      301 ccatcgacgc actcgagctc gtcgtttcac tcgagaagga gttcggcata aaaatcacca
      361 gcagcgagga atcgcgcacc gccctcgcga gcgtgaccca actggccgaa ttcattcgcg
      421 cccacgctcc tcaagaccga ctccccgcct gacgctccgc catgaagccg ctcgcggcgt
      481 ggagcatcgc ccaatgatcg cgtcgcacac gcttcagccc gtcgcgatcg aagcggcagg
      541 ctgtatcacc gccctcggcg acggccccgc cacgtgggcc gcgctcctgc gcaacgaggt
      601 cgcgctccag gagcggcctg tgctcggccc cgacggtggc gacgcagtcc cgctcgcact
      661 cgtgcccgga cgttccctcg aagaggccgc cccgcccgcg tgggccggac tcaccgagcc
      721 actcgtgcgc gccataccag aggatcggtg gggctccgcc cgccgacccg tcttcgtcac
      781 gagcagtaat ttcggtgtcg gtagcctcta cgcgtatcaa cgttccaagg acaccgcgca
      841 cctcgcctac ggcacgccgt ttgccaccgt cgaatttctc cgtcgccgct tcaactggga
      901 ccaaaacgtc gccgtcctct cccacgcctg cgtctccgcg cacctcggtc tgctcctcgc
      961 cgcgcgcacc ctcgccgccg gactcgccga ccgcgcactg gtgttcagct tcgacttcgt
     1021 ttccccattc gtcgccggcg gatttcattc gttgaaaatc ctcaacgggg gattccccgc
     1081 cccctattct gcccgcgcga ccggatcgat cgggctcggc gatggcgcag cgttcgccgt
     1141 cctcacccgc gatccgggcc cgtcctcgac cggcttgcgc ctcgaagccc aggcgctgca
     1201 caacgaaatg caccacttca ctgcgaaccg cgccgatggc agcggctttc gcgcgtgcct
     1261 ggctcctgtt cgcgacgcgg tgggttctcg gcgcatctgg gtcaagggcc atggcaccgg
     1321 aacactcgaa gccgggcggt tggaagccga aacgctcgc
//
LOCUS       DASPLT010000320         1041 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_291469, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000320 DASPLT010000000
VERSION     DASPLT010000320.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1041)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1041)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1041
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_291469"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..991
                     /locus_tag="VEA63_03340"
     CDS             <1..991
                     /locus_tag="VEA63_03340"
                     /inference="COORDINATES: protein motif:HMM:TIGR00757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HYD83051.1"
                     /translation="PPEEKGGFIIRTMAETASDRELHCDVAYLRKLWHGIQERARDAP
                     ALALLYQDLTLSLRVLRDFMHEDTVRILIDSRETFETMREFASEFTPNVVPRLEHYQG
                     ERPLFDLYGVEDEIEKALARRVDLKSGGYLIIDQTEALTTVDVNTGGFVGGRSFDDTI
                     FKTNLEAAQVIARQLRVRNLGGIIIIDFIDMDSDEHRNAVLHEFRKALARDRTRMTVN
                     GFTQLGLVEMTRKRTRESLAHVLCEPCPTCEGRGELKTAQTVCYQILRELLREARQFS
                     AREFRILGSQQVIDMFLDEESQSLAMLSDFIGKPVSLQVESAYNQEQFDVVLM"
BASE COUNT          215 a          325 c          318 g          183 t
ORIGIN      
        1 gccgccggaa gaaaaaggcg gcttcatcat ccgcacgatg gcggagacgg cttcggaccg
       61 cgagcttcat tgcgatgtcg cctacctgcg caagctctgg cacggcatcc aggagcgcgc
      121 acgcgatgcg ccggcgctgg cgctgctcta tcaggatctc acgctgtcgc tacgcgtgct
      181 gcgtgatttc atgcacgagg acaccgtgcg catcctgatc gattcacgcg agactttcga
      241 gacgatgcgc gaattcgcct ccgagttcac gccgaacgtc gttccgcggc tcgagcacta
      301 tcagggcgag cggccgctct tcgatctcta cggcgtggag gacgagatcg aaaaagcgct
      361 cgcgcggcgt gtagacctca aatcgggcgg ctacctcatc atcgatcaga cggaggcgct
      421 caccaccgtc gacgtgaaca ccggcggctt cgtcgggggg cgcagtttcg acgacacgat
      481 cttcaagacc aacctagaag ccgcgcaggt gatcgcacgc cagttgcggg tgcgcaacct
      541 cggcggaatc atcatcatcg atttcatcga catggactcg gacgagcacc gcaatgccgt
      601 gctccatgaa ttccgcaagg ccctcgcacg cgaccgcacg cggatgaccg tcaatggctt
      661 cacgcagctt ggcctggtcg agatgacgcg caagcgcacg cgcgaatcgc tcgcccacgt
      721 gctgtgcgag ccgtgtccta catgcgaagg gcgtggcgag ctgaaaaccg cacagacagt
      781 gtgctatcag atcctgcggg agctactgcg cgaggcacgc cagttcagcg cacgtgagtt
      841 tcggatactc ggctcgcagc aggtgatcga catgttcctg gacgaggagt cacaaagcct
      901 cgccatgctc tcggacttca tcggcaagcc ggtgtcgctg caggtcgaga gcgcgtacaa
      961 ccaggaacag ttcgacgtcg tgctgatgtg atttgaacgc gccgcagact accgttaaaa
     1021 gacgcgcagt aataacgccg c
//
LOCUS       DASPLT010000321         1325 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_249794, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000321 DASPLT010000000
VERSION     DASPLT010000321.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1325)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1325)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1325
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_249794"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1280)
                     /locus_tag="VEA63_03345"
     CDS             complement(<1..1280)
                     /locus_tag="VEA63_03345"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HYD83052.1"
                     /translation="MHTNLHMAEVRRVAAAQVSASEARFEQLVSVLPVGVCACDAAGR
                     VTFVNRRAVEIWGCEPAADESYEKFSKRFKTFTPDGEPVPQTERPLRLALVEGRTVRQ
                     GEAIVERPDGTRFVASFTTAKITDESGGVTGSIAVFQDITGEKRARTALRESEERYRA
                     VFQQASVGIFECELDGRIVRSNPALSRMLGYAPEELVGKNWRELVPAEDLPANDEVAQ
                     QLADGRLQAITSERRYRRKDGTTGWSDVFATTILDANGGIARGLAVLVDASERKRAAA
                     ELRETEARFRAAADSAPVLIWMTTPDKSCAWVNQPWLDFVGQTLEAVLSEGWTKSVHA
                     EDAERTLHVFDEAFRARQPFSMEYRLRRHDGVYRWVLSHGVPRFQGADFVGYIGSCVD
                     IEDRKRAEAEVARARDEAVAASRAKDDFLAALSH"
BASE COUNT          215 a          519 c          365 g          226 t
ORIGIN      
        1 tcgtgcgaca gcgccgcgag aaaatcgtcc tttgcccgcg aagccgccac ggcttcgtcg
       61 cgcgcgcgcg ccacctccgc ctccgcccgt ttccgatcct cgatgtcgac gcacgagccg
      121 atgtagccga caaagtccgc gccttggaaa cgcggcacac catggctgag cacccagcga
      181 tacacgccgt cgtgccgccg cagccggtat tccatgctga acggctgccg cgctcgaaac
      241 gcctcgtcga aaacatgcaa cgtccgctcg gcgtcctccg catgaacgct cttcgtccag
      301 ccttcactca acacggcctc cagcgtttgc ccgacgaagt ccagccacgg ctggttgacc
      361 cacgcgcagc ttttatccgg cgtcgtcatc cagatcagca ccggcgcgct gtccgccgcc
      421 gcacgaaacc gcgcctcggt ctcccgcagc tccgccgcgg cgcgcttgcg ctccgacgcg
      481 tcgaccaaca ccgcgagccc gcgcgcgatg ccgccgtttg catcgagtat cgtcgttgcg
      541 aatacatccg accagccggt cgtcccgtcc ttccgccgat aacgccgctc cgaggtgatc
      601 gcctgcaggc gcccgtccgc gagttgttgc gcgacttcgt cgttcgccgg gagatcctcc
      661 gccggcacca actcgcgcca gttttttccg acgagttctt ccggcgcata gcccagcatg
      721 cggctcagcg cgggattgct ccgcacgatg cggccgtcga gctcgcattc aaaaatgccg
      781 accgacgcct gctggaacac cgcccgataa cgctcctccg attcgcgcaa cgccgtgcgc
      841 gcgcgttttt ccccggtgat gtcctgaaac accgcgatcg aacccgtcac gccgccgctt
      901 tcgtccgtaa tcttcgccgt cgtgaaagac gcgacgaacc gcgtgccatc cggccgctcg
      961 acgatggcct cgccttgccg cacggtgcgg ccttccacca acgcgaggcg cagcggccgc
     1021 tccgtttgcg gcaccggctc gccatcgggc gtgaacgttt tgaaccgctt cgaaaacttc
     1081 tcgtagcttt catcggccgc gggttcgcag ccccagattt caaccgcacg ccgattgaca
     1141 aacgtcacgc gtcccgccgc gtcgcacgcg cacacgccga ccggcagcac cgacacgagc
     1201 tgctcgaaac gcgcctcgct cgcgctcacc tgcgccgccg ccacgcgccg cacctccgcc
     1261 atgtgcagat tcgtgtgcac gcgcgccaac agttcgcgcg ctccgaacgg cttgatcaaa
     1321 tagtc
//
LOCUS       DASPLT010000322         1435 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_26320, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000322 DASPLT010000000
VERSION     DASPLT010000322.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1435)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1435)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1435
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_26320"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..910
                     /locus_tag="VEA63_03350"
     CDS             <1..910
                     /locus_tag="VEA63_03350"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HYD83053.1"
                     /translation="VYATNAPSMPAQIALGAEDYNRLVRMIKQGEKVKMTIDLQVAFH
                     DEDLLGYNTVAEIPGSDLKDEVVMLGAHLDSWHCGTGATDNAAGVAAAMEALRILRAL
                     DLQPRRTIRVALWTGEEQGLLGSRGYVAKHFGFYTNMTNVAARSPRAQANESASARSS
                     RTSSTRKLIRHANYEKFSAYYNLDNGTGKIRGVYMQGNEAVRPLFRKWLQPFNDLGAE
                     TLTLANTGGTDHLSFDGIGLPGFQFIQDPIDYFTRTHHSTADVFDRIQADDMKQAATI
                     MAAFAYNTAMLETTLPRKPLAENRSR"
     gene            1010..>1435
                     /locus_tag="VEA63_03355"
     CDS             1010..>1435
                     /locus_tag="VEA63_03355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD83054.1"
                     /translation="MAIALNPFRAIAFYMLLAVSVAPSATFAQDRLKTMPGYERHQRI
                     TRASSNAFKTGAISATWTNDGAAFIYRRDNRRFLYDLAARRATELAPSTNRSMRSARP
                     RNVPRPPLRGRQYTKAISPDKKFTAEYRDRNVWLTTEKDT"
BASE COUNT          286 a          490 c          412 g          247 t
ORIGIN      
        1 cgtttacgcg accaatgcgc cgtccatgcc cgcccaaatc gcgctcgggg ccgaggatta
       61 caaccggctc gtgcggatga tcaagcaggg cgagaaggtg aagatgacga tcgatctgca
      121 agtggctttc cacgacgagg atctgctggg ctacaacacg gtcgcggaga tcccgggcag
      181 cgatctcaag gacgaggttg tgatgctggg cgcgcatctc gattcctggc attgcgggac
      241 tggcgcgacc gacaatgccg cgggcgtggc ggcggcgatg gaggcgttgc gcatccttcg
      301 cgcgctggac ctgcagccgc gccggaccat tcgcgtggcg ctctggacgg gcgaggagca
      361 agggctgctt ggctcgcgtg gttatgtggc gaaacacttc ggtttttaca caaacatgac
      421 gaacgtggcg gcgcgctcac cgcgggcgca agccaacgaa agcgccagcg cgcgctcctc
      481 ccgcaccagt tccacccgca agctcattcg ccatgcgaac tacgagaagt tttccgctta
      541 ctacaatctc gacaacggca ccggcaaaat tcgcggcgtc tacatgcaag gcaatgaggc
      601 ggtgcgtccc ctgttccgca aatggctcca gcccttcaac gatctgggcg cggaaacgct
      661 gaccctcgcc aacaccggcg gcacggacca cctttccttc gacggcatcg gcctgcccgg
      721 attccagttc atccaggacc cgatcgatta tttcacccgc acccatcatt ccacggccga
      781 tgtgttcgat cgcatccagg ctgacgacat gaaacaggcc gcgaccatca tggcggcgtt
      841 cgcctacaac acggccatgc tcgaaacgac attgccccgc aagccgctcg cggaaaatcg
      901 gagccgttga tctcgtccgc tcgcaccatt cgcgccggtt atgatctctg agccacgggt
      961 tggatcttcc cgcgggcggt cgcatcattc actgtcaccg catccattta tggccatcgc
     1021 tctgaatcca ttccgcgcca tcgcgttcta catgctcctt gccgtcagcg tcgcgcccag
     1081 cgcgacgttc gcgcaggacc ggctgaaaac gatgccgggc tacgaacgcc atcaacgcat
     1141 cacgcgcgcg tcctcgaacg ccttcaaaac cggcgccatc tccgcgacat ggaccaacga
     1201 cggagccgcc ttcatctatc gccgggacaa caggcgattt ctctacgacc ttgccgcgcg
     1261 ccgcgcgacg gagctggctc cctcgacgaa tcgttccatg cgcagcgcgc gcccccgcaa
     1321 cgtcccccgt ccaccgctgc ggggacggca atacacgaag gccatttcgc ccgacaaaaa
     1381 attcacggcg gaataccggg accggaacgt gtggctgacc acggagaagg acacc
//
LOCUS       DASPLT010000323         1207 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_188211, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000323 DASPLT010000000
VERSION     DASPLT010000323.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1207)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1207)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1207
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_188211"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..268)
                     /locus_tag="VEA63_03360"
     CDS             complement(<1..268)
                     /locus_tag="VEA63_03360"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HYD83055.1"
                     /translation="MKAIPDPLPGPSGTRIRTIIVDDEPAARRGVRLLLERDHGIEIV
                     GEAGSGIEAMELINRERPALAFLDVQMPGCDGFETLARVGTAAAP"
     gene            complement(265..>1207)
                     /locus_tag="VEA63_03365"
     CDS             complement(265..>1207)
                     /locus_tag="VEA63_03365"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HYD83056.1"
                     /translation="VPQYGRAAMWAMLAPLILRMRERCPLNRGGWVGGVSFHLFASFV
                     VMATYYLGRMWSYSVYFKEFQEGFWVTAAKGFYGRNIIDMAYYWAVIAFGYSFEIYQK
                     YKSEELKAAQLEARLIETELNALRQQMHPHFLFNTMNTIAVLVREGRNDDAVTLLARL
                     SSLLRMSLDNTGVPEVTLQQELDFLGHYIEIQKARFSDRLNVDIAIAPEALRARIPNL
                     LLQPIVENAILHGVAPKAAPGRVEVTGRVEGDRLLLEVCDDGPGIAANGGRKREGVGL
                     ANTRERLLKTYGARASLTLNSDPGCGVRVQIVLPFRT"
BASE COUNT          223 a          435 c          329 g          220 t
ORIGIN      
        1 ccggcgccgc cgccgtcccg acgcgcgcga gcgtttcgaa tccatcgcaa cccggcatct
       61 gcacgtcgag aaacgccagc gccggccgtt cgcggttgat caactccatc gcctcgatgc
      121 cgctcccggc ttcgccgaca atctcgattc cgtggtcgcg ttcgagcagg agtcgcactc
      181 cgcgtcgcgc ggccggttcg tcgtcgacga tgatcgtgcg gatgcgcgtg ccggaaggtc
      241 caggcagagg atcggggatg gctttcatgt gcggaaggga aggacgattt gcacgcgcac
      301 gccgcagccg gggtcgctgt tcaacgtcag gctcgcgcgc gctccatacg ttttcaacag
      361 ccgctcgcgc gtgttggcga gccccacgcc ttccctcttg cgcccgccgt tcgccgcaat
      421 ccccggcccg tcgtcacaca cttcaagcag caaacggtcg ccctcgacgc gtcccgtgac
      481 ctccacccgc ccgggcgccg ccttcggcgc gaccccgtgc aaaatcgcgt tttccacgat
      541 cggttgcagc agcagattcg gaattcgcgc gcgcaacgcc tccggcgcga tcgcgatgtc
      601 cacgttcaac cggtccgaaa accgcgcctt ctggatctcg atgtagtgcc cgagaaaatc
      661 caactcctgc tgcagcgtca cctccggcac gcccgtgttg tcgagcgaca tccgcagtaa
      721 cgagctcaac cgcgccagca acgtcaccgc gtcgtcgttg cgcccttcac gcacgagcac
      781 cgcgatcgtg ttcatcgtgt tgaagagaaa atgcggatgc atctgctgtc gcaacgcatt
      841 gagctccgtt tcgatcagcc gcgcctcgag ctgcgccgcc ttcaattcct cgctcttgta
      901 tttctggtaa atctcgaagc tgtagccgaa cgcgatcacc gcccagtaat acgccatgtc
      961 gatgatgttg cgtccgtaga aacccttcgc cgccgtcacc caaaagcctt cctggaattc
     1021 tttgaaataa accgagtagc tccacatccg gccgagataa tacgtcgcca tcaccacgaa
     1081 gctggcgaac aagtggaagc tcactccgcc cacccagccc ccgcggttga gcggacaacg
     1141 ctcgcgcata cgcaggatca acggcgccaa catcgcccac atcgccgcgc gaccatattg
     1201 cggcacc
//
LOCUS       DASPLT010000324         2488 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_228664, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000324 DASPLT010000000
VERSION     DASPLT010000324.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 2488)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2488)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2488
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_228664"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(215..832)
                     /locus_tag="VEA63_03370"
     CDS             complement(215..832)
                     /locus_tag="VEA63_03370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD83057.1"
                     /translation="MKTSLLTLALPFVGALAFAAELAPTRATLQAPTAAPAARAPTNA
                     STAANTNGADATDRPNATAGISGDTSAKVNEGLPKFTPPPPAPPPSPVGTPAPLPAET
                     ASTDFTDNSVIRLPNYDVREQELPEFKERELLTREGRIDAAMTRFPGLKFGPFDQLNA
                     RVGLVLLEQEHEVERRTELRELYDFMAFMRSVPRATANPEPAAAE"
     gene            complement(882..1514)
                     /locus_tag="VEA63_03375"
     CDS             complement(882..1514)
                     /locus_tag="VEA63_03375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD83058.1"
                     /translation="MHRSVRRNVVFPLLAALALTATAVAQSTPPAVAAKSDATPAETP
                     RPKRERAISNNVASALAASMPKYNPPPKPRPEDQDVDLRDVDKPRNGIIRLPRYTVRE
                     RKPPVFRERDIHTNAGFAELARRRYLTPTYRLLNSVYIPFLTASPTQHALAMYAEDER
                     LANMADIEDDAQTIQRVDRDKAAYLRRVSNDTYMRSSGFGYNPNGLDRTE"
     gene            complement(1658..2188)
                     /locus_tag="VEA63_03380"
     CDS             complement(1658..2188)
                     /locus_tag="VEA63_03380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HYD83059.1"
                     /translation="MKFIVLHGRPAVGKLAVARELAQLSQWRLFHNHLVVDALLAVFD
                     FGSAPFVELRESIWHDVFLRASTSGVRGLIFTFNPENTVHPSFIDRLVADARARGDDL
                     HFIELTCSAAEAEQRLTAASRLDTGKLTSVELYRKLVSEGAFDQPRLPPPDFSLDTGS
                     LPPAEAARQIWQRLNR"
     gene            complement(2218..>2488)
                     /locus_tag="VEA63_03385"
     CDS             complement(2218..>2488)
                     /locus_tag="VEA63_03385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD83060.1"
                     /translation="LFAVLPRLKPGVVVHFHDIFDGFEYPKAWLDEGRYWNEQYALRA
                     FLMFNHAFKILLHTASVFNRFNDFFRERLPVTLRHGGGQLWLQRV"
BASE COUNT          443 a          631 c          973 g          441 t
ORIGIN      
        1 ggcgcagccg gccggcgacg gatgtgttgc cgaaaacgga gtgggagcag atcgcgcgcg
       61 aattgctggg tgatcggccg aaggcggagt gaacgcgatt cgacgacgct accgaagtcc
      121 gagataagag gcgcgttggt gtaatcgaat gtccgcggtg gacgcggaca gctcggagag
      181 cagtccctac cgacgttggc gccccaagct gtggctattc ggcggcggcg ggctcggggt
      241 tcgcggtggc gcgcgggacg cttcgcatga aggccatgaa atcgtagagt tcgcgaagct
      301 cggtgcggcg ctccacttcg tgctcctgct cgagcaatac gagaccgaca cgggcgttga
      361 gctggtcgaa agggccgaat ttgaggccgg gaaaacgcgt catcgcggcg tcgatgcgtc
      421 cttcgcgagt gagcagttca cgctctttga attcggggag ttcctgctcg cggacgtcgt
      481 agttaggcaa ccggatgacg ctgttatctg tgaaatcggt gctggcggtt tcggcgggca
      541 gcggcgccgg ggtgccgacg ggactcggcg gcggggccgg cggaggcggg gtgaacttcg
      601 gcaaaccttc gttgaccttt gccgaggtgt cgccggaaat gccggctgtc gcgttcgggc
      661 ggtcggtggc gtccgcgccg ttggtgttgg ctgccgtgga ggcgtttgta ggagcgcggg
      721 cggcgggtgc tgcggtgggc gcttggagcg tggcgcgggt gggggcgagt tcggcggcga
      781 atgcgagcgc gccgacgaac ggaagagcga gggtgaggag tgaagttttc atggcggcga
      841 cggggaaacg gaggtctcgc gcggcgccgc tggcagcgga cctactcggt gcgatcgagt
      901 ccgttcgggt tgtagccgaa accgctcgag cgcatgtagg tgtcgttgct cacgcggcga
      961 agataggcgg ctttgtcgcg gtcgacgcgc tggatggttt gcgcgtcgtc ctcgatgtcg
     1021 gccatgttcg cgagccgttc atcttccgca tacatcgcga gcgcgtgctg cgtcggcgaa
     1081 gcggtgagga acggaatata aacggagttc agaaggcggt aggtgggcgt caggtaacgg
     1141 cggcgggcga gttcggcgaa gccggcgttg gtgtggatgt cgcgttcgcg aaacacgggt
     1201 ggcttgcgtt cgcggacggt ataacgggga aggcggataa tgccgttgcg aggcttgtcg
     1261 acgtcgcgca ggtcgacgtc ctgatcttcg gggcgtggct tgggcggcgg gttgtatttc
     1321 ggcatgctcg cggcgagcgc ggaggcgacg ttgttggaaa tcgcgcgttc gcgttttgga
     1381 cggggcgttt ccgcgggcgt agcgtcggac ttcgcggcga cggcgggcgg cgtcgattgc
     1441 gcgacggcgg tcgcggtgag cgcgagggcg gcgaggagcg gaaaaacgac gttgcggcgg
     1501 acggagcgat gcatggcgaa ttaagtcaat ttagcctctg cgcggttccg cgcaaccctg
     1561 agcatgaaaa cgaggatgac ggaacaatct gctggagcgg gcctgaggag tgttgaaatg
     1621 gcgggtgttg atccgcggac ggcggaggcg tggcggatca gcgatttaga cgctgccaga
     1681 tctggcgggc ggcttcggcg ggcggaaggc tgccggtgtc gagggagaaa tccggcggcg
     1741 ggaggcgcgg ctgatcgaag gcgccttcgc tcaccagttt tcggtagagt tcgacggacg
     1801 tgagctttcc cgtgtcgagg cgcgatgcgg cggtcagtcg ctgctcggct tcggctgcgg
     1861 agcaagtgag ctcgatgaag tgcagatcgt cgccgcgcgc ccgggcgtcg gcgacgagcc
     1921 ggtcgatgaa ggacgggtga acggtgtttt caggattgaa cgtgaagatc aagccgcgca
     1981 cgccgctcgt gctcgcgcgc agaaagacat cgtgccagat gctctcgcgc agctcgacga
     2041 acggtgcgct gccgaagtcg aacacggcga ggagagcgtc gaccacgagg tgattgtgaa
     2101 acaaacgcca ctggctgagc tgcgcgagtt cacgagcgac ggcgagtttg ccgacggcgg
     2161 ggcgaccgtg aagaacgata aatttcatgg gatgcgaccg aaaaaaatcg cgcgcgatca
     2221 gacgcgctgc aaccaaagtt gtccgccgcc gtgtcgcaag gtcacgggca ggcgctcgcg
     2281 gaagaagtcg ttgaaccgat tgaagacgga ggcggtgtga agcaggattt tgaacgcgtg
     2341 gttgaacatc aggaaggcgc gcagcgcgta ttgctcattc caataccggc cctcgtcgag
     2401 ccaggccttg ggatactcga aaccgtcgaa gatatcgtga aagtggacga cgacgccggg
     2461 tttcaaacgc gggagcaccg cgaaaaga
//
LOCUS       DASPLT010000325         1498 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_105549, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000325 DASPLT010000000
VERSION     DASPLT010000325.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1498)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1498)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1498
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_105549"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..229)
                     /locus_tag="VEA63_03390"
     CDS             complement(<1..229)
                     /locus_tag="VEA63_03390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD83061.1"
                     /translation="MGANLLIVGQGLAGTLLAWELERAGLTFEIADEGHGRAASRVAA
                     GIINPITGQRLVKSWRVDALLPVARATFRAIE"
     gene            complement(499..741)
                     /locus_tag="VEA63_03395"
     CDS             complement(499..741)
                     /locus_tag="VEA63_03395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD83062.1"
                     /translation="MMGSMSFSCPFYVPDGDYCLRLKTDCVPGRRGCVLPANTQYAVP
                     AEVRVRAKEEEKRQANRDVLPPWPPTGKGYEGATRA"
     gene            841..1413
                     /locus_tag="VEA63_03400"
     CDS             841..1413
                     /locus_tag="VEA63_03400"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008725 - DNA-3-methyladenine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HYD83063.1"
                     /translation="MPSPTLKRCPWPTKPLYIAYHDTEWGVPLHDDRALFELLTLEGA
                     QAGLSWSTILAKRENYRRAFDHFDATKIARYDARKTAALLADAGIVRNRLKIAATIQN
                     AKAFLAVQREFGSFDRYLWSFVGGRPIVNRRRSMRDVPARTSESDALSRDLLKRGFKF
                     VGTTICYAFMQASGMVNDHLVTCPRHAACQ"
BASE COUNT          286 a          595 c          358 g          259 t
ORIGIN      
        1 gctcgatcgc acgaaacgtc gcgcgcgcca ccggcagcag cgcgtcaacc cgccaactct
       61 tcaccagccg ctgacctgta atgggattga tgatacccgc cgcgacccgc gacgccgcgc
      121 gcccgtgccc ttcgtccgcg atttcgaagg tcaaccccgc ccgctccagc tcccacgcga
      181 gcagcgttcc cgccaaacct tgaccgacga tgaggaggtt tgcacccacg cgcgaaaaaa
      241 accgaaagcc caccgagccc gcaacaaact ccgtccaaca atccgcgccc caaaaaacgc
      301 cgtcagaaat tcttctcttc cccatctcgg cgccaaaccg ccaatgtagg agcctgcttg
      361 cagatgatag gatgcgttcg cctacgctcg gtgcgcgcaa tcgcgacaag ccggtttcct
      421 tttacctacc accctccgcc tctgcgttcg acgcaacccc aacgcctgat gacgcctgct
      481 ctgcctatgc ccccgcaatc acgcacgcgt cgcgccctcg taacccttcc ccgtcggagg
      541 ccacggcggc agcacatcgc ggttcgcttg ccgcttctcc tcttccttcg cccgcacccg
      601 cacctccgcc ggcaccgcat actgcgtgtt ggccggcaac acgcaccctc ggcgccccgg
      661 cacgcaatcg gttttcaagc gcaggcagta atcgccgtcg ggcacataaa atggacagga
      721 aaaactcatc gaccccatca tcgctcagcg gcgcacgccg cgccttgatc tcgctcaagc
      781 tctcagcctc ttcatcaatc ctgacatcac gctgtcgcac acctgcccca tcctgtccag
      841 atgccttcgc ctaccctcaa acgctgcccc tggcccacca aaccgctcta cattgcttac
      901 cacgataccg agtggggcgt tccgctccat gatgatcgtg cgctgttcga actcctcacc
      961 ctcgaaggcg cgcaagccgg cctgagttgg tcgacgattc tcgcgaaacg cgaaaactac
     1021 cgccgcgcct tcgaccattt cgacgccacc aaaatcgctc gctacgacgc gcgcaaaacc
     1081 gccgcgctcc tcgccgacgc cggcatcgtg cgcaatcgcc tgaagatcgc cgccacgatc
     1141 caaaacgcga aagccttcct cgccgttcag cgcgagttcg gcagtttcga tcgctacctg
     1201 tggtccttcg tcggcggccg cccgatcgtg aatcgccgcc gttccatgcg cgatgtcccc
     1261 gctcgcacgt cggaaagcga cgcgcttagc cgcgacctgt tgaagcgcgg cttcaaattc
     1321 gtcggcacca cgatctgcta cgcgttcatg caagccagcg gcatggtcaa cgaccacctc
     1381 gtgacctgtc cccgccacgc cgcctgccag tagggccggg cttcaacccg ccttccgcaa
     1441 agcgcgcgtt cattttctcc cgccgctccc cccgaaacaa agaaccccgc cagtcgcc
//
LOCUS       DASPLT010000326         3905 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_188256, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000326 DASPLT010000000
VERSION     DASPLT010000326.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 3905)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3905)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3905
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_188256"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..200)
                     /locus_tag="VEA63_03405"
     CDS             complement(<1..200)
                     /locus_tag="VEA63_03405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD83064.1"
                     /translation="MLRLHLPPNLAAAAPRDAIPLKVELDLSTPPALELLPALALLQR
                     WCGSATPPKFIQLTRAQLRELAA"
     gene            348..899
                     /locus_tag="VEA63_03410"
     CDS             348..899
                     /locus_tag="VEA63_03410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HYD83065.1"
                     /translation="MSSDVFTQLVDAHYAPLYRFALSLAKKPADAGDLVQQTFFIWAT
                     KGHGLRETAKAKSWLFTTLYREFLRGRRRDSRATSLEDLPPGEADVTAEEVDRVARMD
                     AASVVAALQEVDEVFRAPLTLFYLQDLSYQEIADTLDVPIGTVMSRLSRGKAQLRAAL
                     AREESAARAKVVPFTGAKGRTQA"
     gene            896..1624
                     /locus_tag="VEA63_03415"
     CDS             896..1624
                     /locus_tag="VEA63_03415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD83066.1"
                     /translation="MTNEEAKFILGGYRGNGRDASDTLFAEALKQAQADPGLGAWFAR
                     EQAHNAAVAAKLAEIAPPAGLREAILAGGRVSVSAKPWWVRREWLAMAAAVAVAIGVG
                     TMTLWTSRATAAGDPLASYALADVKPHRGHGAAVAALQTAMKNPAMRLSAALPVDFAE
                     LERSGCRTVLIGKRPVLEVCFEREGRWFHCYIGRCEDFPELAAQQAPEFASSGEMTAA
                     TWANGTYRVVVAGRAGREVIERLL"
     gene            complement(1802..>3905)
                     /locus_tag="VEA63_03420"
     CDS             complement(1802..>3905)
                     /locus_tag="VEA63_03420"
                     /inference="COORDINATES: protein motif:HMM:TIGR01783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0015343 - siderophore-iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent siderophore receptor"
                     /protein_id="HYD83067.1"
                     /translation="STPRLGPPAPAPPNPPAPEEVIRLPTFTLREGRDFSYVGKQALS
                     TTRTGVELLDLAQSVKVINRAFIDDLSPGLLVDTLKYVGGGQAGNINFADDRFTLRGF
                     NSPADIGDFVDGFRGKTDSNTDLAIIERLEIIKGPSAIFVANGPVGGVINKVIKGPVS
                     YPLGTLKLQTGLFDANRAELDLGGPLTADGKILYRLVAAGQYSDGWYDRTYAHRLVVA
                     PSIAYVFNEHSRITLKYNYLLYRFSSYNGLPFDERTGRMLAIDRQAHFGEDAPRNWRK
                     DIVHRVFLEYTNRFNDHLAMRIAGFNSYNHAARIESVNGAALPANYVPGTLIARSTTA
                     QDREHDRRQLQTDLVATFSTGPLDHRLLVGGELADAPDHVASFTGTSSAIYPFDLQFP
                     GTVTVNTAAPGSYLRTNNRQNKFFALETLSVLDGRLQFSFGGSKVKAITSSHNKLTDV
                     STTRLELNQTLRQHGLVYKVTPHVSLFYGYNENFAPNFLNGQVLPSQLGSQREFGVKT
                     DILDGRLQMNIAYFDIEQENVPVLSFPQTTPPTFVLVPGQTSKGVDGDITFQATKNLD
                     LVFTFADMNAKARSQANSAAPVIVNPVNNVAEQTFGLWTRYKITHGAAKGFSIGLGVS
                     HLAKRAIASNNNAIVYGWLDAFTLTDLAIAYDRGAFRYGLNVDNVFDVDYEAAVRNQS
                     IIVPGMGTNVKASVTWKF"
BASE COUNT          719 a          926 c         1538 g          722 t
ORIGIN      
        1 gcggcgagtt cgcggagttg ggcgcgggtg agctggatga acttcggcgg cgtggcggag
       61 ccgcaccagc gctggaggag tgcgagggcg ggaaggagct cgagggcggg cggggtggag
      121 agatcgagtt ccaccttcaa cggaatcgca tcgcgcggag cggcggcggc gaggttgggt
      181 gggaggtgga gacgcagcat cggagtgaga gggagagtga aagtgaaagg gactgaggga
      241 gtgagggaat ggggggagtg aaagggggtt tgggggttgc ggggtgggca atgacaaagt
      301 ggagtggcgg gaataaacga ggacgcggtg gcctcgtagc acaaacgatg agctccgatg
      361 tcttcacgca actggtcgat gcgcactatg cgccgctcta ccggtttgcg ttgagcctgg
      421 cgaaaaaacc ggcggatgcg ggcgatttgg tgcagcagac gtttttcatc tgggcgacga
      481 aggggcacgg cttgcgggaa acggcgaaag cgaagtcgtg gctcttcacg accttgtatc
      541 gcgaattttt gcgcgggcgg cggcgggact cgcgggcgac gtcactggag gatttgccgc
      601 ccggcgaggc ggatgtgacg gcggaggaag tggatcgcgt cgcgcggatg gacgcggcgt
      661 cggtcgtggc ggcgttgcag gaagtggacg aggtgtttcg cgcgccgctg acgttgtttt
      721 atttgcagga tctttcgtat caggaaatcg cggacacgct ggacgtgccg atcgggacag
      781 tgatgtcacg gctgtcgcgc ggaaaagcgc agttgcgagc ggcgctggcg cgggaggaga
      841 gcgcggcgcg ggcgaaagtg gtgccgttca cgggcgcgaa ggggagaaca caagcatgac
      901 gaacgaagag gcgaaattta ttctcggcgg ttatcgcggc aatggtcgcg atgcgagcga
      961 tacgctgttc gcggaggcgt tgaagcaggc gcaggcggat ccgggattgg gggcgtggtt
     1021 tgcgcgcgaa caggcgcaca acgcggcagt ggcggcgaag ctcgcggaga tcgcgccgcc
     1081 ggcgggattg cgcgaagcga ttttggcggg cgggcgcgtg agcgtgagtg cgaagccgtg
     1141 gtgggtgcga cgggagtggc tcgcgatggc ggcggcggtc gcggtggcga tcggggtggg
     1201 aacgatgaca ttgtggacct cgcgtgcgac ggcggcgggc gatccgttgg cgagttatgc
     1261 gctggcggat gtgaagccgc atcgcgggca cggcgcggcg gtggcagcgt tgcagacggc
     1321 gatgaaaaat ccggcgatgc gcttgagcgc ggcgttgccg gtggattttg cggagctgga
     1381 gcgatccggc tgccggacgg tgctgatcgg gaaacggccg gtgctcgaag tgtgcttcga
     1441 gcgcgagggt cggtggtttc actgctacat cgggcgatgc gaagattttc cagaattggc
     1501 ggcgcagcag gcgccggagt ttgcctcgag cggcgagatg acagcggcca cgtgggcgaa
     1561 cgggacgtat cgtgtggtcg tcgcgggtcg cgcgggccgg gaagttatcg agcgattact
     1621 atagggcgcg tggcgttagc atagagcggg agagccattg gaaaaaggaa gccggcggcg
     1681 accaaccgcc ggcttcacaa gctactccgc taaccaacta acgactgacc ttactgacta
     1741 tccaactgac cgactaacga acgggattga cggcgtgtgc cgcaaaaagt gacgggcgaa
     1801 gctagaattt ccaggtgacg gaagccttca cgttcgtgcc catgccgggg acgatgatgc
     1861 tttggttgcg cacggcggct tcgtaatcga cgtcgaacac attgtcgacg ttgagaccgt
     1921 agcggaaagc tccacggtca taggcgatcg cgagatcggt gagcgtgaag gcgtcgagcc
     1981 agccgtagac gatcgcgttg ttgttgctcg caatcgcgcg cttcgcgagg tggctcacgc
     2041 cgaggccaat cgagaagccc ttcgcggcgc catgagtgat cttgtagcgg gtccacagac
     2101 cgaaggtttg ctcggcgacg ttgttgaccg ggttgacgat gacgggcgcg gcggagttgg
     2161 cttgcgaacg cgctttcgcg ttcatgtcgg caaacgtgaa aacgagatcg aggttcttcg
     2221 tcgcttgaaa cgtgatgtcg ccgtcgacgc ctttcgaagt ctggccgggc acgagcacaa
     2281 aggtgggcgg cgtggtttgc gggaaggaga ggacggggac gttctcctgc tcgatgtcga
     2341 agtaggcgat gttcatctgc aggcgcccgt cgaggatgtc ggtcttcacg ccgaattccc
     2401 gctgtgaacc gagctggctg gggagcacct ggccgttcag gaaattgggc gcgaagtttt
     2461 cgttgtaacc ataaaacaac gacacgtgcg gtgtgacctt gtagacgaga ccgtgctggc
     2521 ggagcgtttg gttgagctcg aggcgggtcg tcgaaacgtc ggtgagtttg ttgtgcgacg
     2581 aggtgatcgc tttcactttc gagccgccga aggaaaattg gaggcggccg tcgagaacgc
     2641 tgagcgtttc gagggcgaag aatttatttt ggcggttgtt cgtgcgaaga tagctgcccg
     2701 gcgcggcggt gttgacggtc acggtgccgg gaaattggag atcgaacgga tagatcgcgc
     2761 tgctcgtgcc ggtgaagctc gcgacgtggt cgggagcgtc ggcgagttcg ccgccgacga
     2821 gcaggcgatg atcgagcggt ccggtggaaa acgtggcgac gagatccgtt tgaagctggc
     2881 gtcggtcgtg ttcgcgatcc tgggccgtgg tgctgcgggc gatgagcgtg ccggggacgt
     2941 agttcgcggg cagggcggcg ccgttgacgg attcgatgcg agcggcgtgg ttgtaactgt
     3001 tgaaccctgc gatgcgcatc gcgaggtggt cgttgaagcg gttcgtgtat tcgagaaaga
     3061 cgcgatgaac gatgtccttc cgccaattgc gcggcgcgtc ctcgccgaag tgcgcctggc
     3121 ggtcgatcgc gagcatgcgg cccgtgcgtt cgtcgaaggg gaggccgttg taggaggaaa
     3181 accgatacag gagataattg tattttagcg tgatgcggga gtgttcgttg aacacgtagg
     3241 cgatcgacgg cgcgaccacg aggcggtgcg cgtaggtgcg gtcataccag ccgtcggaat
     3301 attgtccggc ggcaacgaga cggtagagga ttttgccgtc ggcggtgagc gggccgccga
     3361 gatcgagctc ggcgcggttc gcatcgaaga ggccggtctg aagtttcagc gtgccgagag
     3421 ggtaggagac ggggcctttg atgaccttgt tgatgacgcc gccgacggga ccgttggcga
     3481 cgaagatggc ggacgggcct ttgatgattt cgaggcgctc gatgatcgcg aggtcggtgt
     3541 tggaatcggt tttgccgcga aaaccgtcga cgaaatcgcc gatgtcggcg gggctgttga
     3601 agccgcggag ggtgaaacga tcgtcggcga aattaatgtt accagcctgg ccgccgccga
     3661 cgtatttcaa cgtgtcgacg aggaggccgg ggctgaggtc gtcgatgaac gcgcggttga
     3721 tgaccttcac ggactgcgcg aggtcgagca gctcgacgcc ggttcgggtg gtgctgagcg
     3781 cttgtttgcc gacgtagcta aagtcgcgac cttcacgaag ggtgaaggtc ggcaggcgaa
     3841 tgacttcctc gggcgcggga ggattgggcg gggcgggagc gggcggaccg aggcgcggag
     3901 tggag
//
LOCUS       DASPLT010000327         3109 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_26362, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000327 DASPLT010000000
VERSION     DASPLT010000327.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 3109)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3109)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3109
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_26362"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..637)
                     /locus_tag="VEA63_03425"
     CDS             complement(<1..637)
                     /locus_tag="VEA63_03425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD83068.1"
                     /translation="MNLSPVRIVVFITSLIVAASLSAQEAAAPSTIGAWRIAGEPQRV
                     MIVTPNYWTQTVFDREQRQFVRTMGGTYSVSGDVARGTLEFDSQEPQGVGQEFRVRTE
                     IKGDALRVTQEDGSDEVWTRIDSAENALAGVWRISGRHADGKLQEMPLRARRTLKILS
                     GTRFQWVAMNVETGEFSGTGGGTYTFENGKYTEHIEFFSRDATRVGAKLEFD"
     gene            814..1509
                     /locus_tag="VEA63_03430"
     CDS             814..1509
                     /locus_tag="VEA63_03430"
                     /inference="COORDINATES: protein motif:HMM:NF013117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel domain-containing
                     protein"
                     /protein_id="HYD83069.1"
                     /translation="MRLSDVTDRFLEYLPRLLGAVPLALAIMVGAFLLNLLIGRALIL
                     LARRTNSTEMDVLPIRHIGRWVVRIVATILILSVFGFQIGGIWAMLSTILGLVAIGFV
                     AVWSLISHTSATMLILFVRPFQIGDDVEFAGEPVNGRVADINFFFTTLVDHEGTLHQI
                     PNNLFFQKTLRRRKNAPVVSLAAQLHSPQPIPVEPPPPPAPKRDALPAKDANAMMMLP
                     DPRSITPPGRPGR"
     gene            complement(1556..2314)
                     /locus_tag="VEA63_03435"
     CDS             complement(1556..2314)
                     /locus_tag="VEA63_03435"
                     /EC_number="2.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016432 - tRNA-uridine
                     aminocarboxypropyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-uridine aminocarboxypropyltransferase"
                     /protein_id="HYD83070.1"
                     /translation="MSRPMCYRCFWPKAQCWCASITPMPTRTKFVFLMHPYEFKRVKA
                     ATGRLTHLCLAESAIHVGIGFDQHEAVQALINDPENYPVLLYPTRDARDLSKGELHAA
                     DFEGRRLVVFLLDATWRLVRRMWRESATLQRLPRIMFSNAAPSRYVIKQQPEPGCLST
                     LEATHELLLALERSGLDRYPLPEQLLGLFQRMQDFQLACAAETARLGIRRHALRTATE
                     RPRSPRPISAKRRRIFAPTSENAGADGGRAADAR"
     gene            complement(2546..>3109)
                     /locus_tag="VEA63_03440"
     CDS             complement(2546..>3109)
                     /locus_tag="VEA63_03440"
                     /inference="COORDINATES: protein motif:HMM:NF013704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HYD83071.1"
                     /translation="SLPDVSEVGFALPEWRSWFLDAVRRVIDAVPDESAAVFFQSDIK
                     RDGAWVDKGAMVVRAAEDAGARVLFHKIVARRPPGLLTLGRPGFTHLITVSRAMRCPD
                     ELAIPDIIVDAGRQPWVRAMGIRAAAHAVRFARDQVGSRLVFDPFCGVGTVLAVANAM
                     GLDALGVEKSRKRCEHARTLTIGAAEL"
BASE COUNT          581 a         1109 c          870 g          549 t
ORIGIN      
        1 cgtcgaattc gagcttcgcg cccacgcgcg tggcgtcgcg cgagaaaaac tcgatgtgct
       61 ccgtatattt gccgttctcg aaggtatacg tgccgccgcc ggtcccggaa aattctccgg
      121 tctcgacgtt catcgccacc cattgaaagc gcgtccccga aagaattttc aacgtgcggc
      181 gcgcccgcaa cggcatctcc tgcaatttcc cgtcggcatg gcggccgctg attcgccaca
      241 cgccggcgag cgcattttcc gcactgtcga ttcgcgtcca cacctcgtcg ctgccgtcct
      301 cctgcgtgac ccgcagcgca tctcctttga tctccgtgcg cactcgaaat tcctgcccca
      361 ctccttgcgg ttcctgcgag tcgaactcca gcgtgccgcg cgccacatcg ccgctgacgg
      421 aatatgtccc gcccatcgtg cgcacgaact ggcgctgctc gcgatcgaac acggtttgcg
      481 tccaataatt cggcgtgacg atcatcacgc gctgcggttc gccggcaatg cgccaggctc
      541 cgatcgtcga gggcgccgcg gcctcttggg cggataggct cgccgccacg atcaacgacg
      601 taatgaaaac aacgatacgc acggggctca ggttcatggg gatgaactcc actgtgcaac
      661 tccgtcgcga cccgtcaaac gccgcaaggc gcggacatgg cgacgccggg tggcgcaatc
      721 gacctttgac atctggccgg agtgcgcttg gtgaacgtca aacccgtggg gattcatcgc
      781 ctcacccgtc gaaacaccca tcctaccgac accatgcgct tatccgacgt caccgatcgc
      841 ttcctcgagt atcttccccg gctgctgggt gccgttccgc tcgcgctggc tatcatggtc
      901 ggggccttcc tgttgaatct tctgattgga cgcgcactca tcttgctcgc ccgccgcacg
      961 aactcgacgg aaatggacgt ccttccgatc cgccacattg gccgctgggt ggtgcggatc
     1021 gtcgcgacaa ttctgatcct tagcgtcttc ggatttcaaa tcggcgggat ttgggcgatg
     1081 ctctcgacga tcctcggcct ggtcgcgatc ggcttcgtcg ccgtatggag cctgatcagt
     1141 cacacctcgg cgacgatgct gatcctgttc gttcgcccgt ttcaaatcgg cgatgacgtg
     1201 gagttcgcgg gcgagccggt gaatggacgc gttgccgata tcaatttttt cttcacgacg
     1261 ctcgtcgatc acgagggaac gcttcaccag attcccaaca accttttctt ccagaaaaca
     1321 ctccggcgcc gcaaaaacgc gccggtggtc agtctcgccg cacagcttca ttcgccgcag
     1381 ccgattccgg tcgaaccgcc gccgccgccc gcccccaagc gcgacgccct tccagccaaa
     1441 gacgcaaatg cgatgatgat gcttcctgat ccacgcagca tcacgcctcc cggtcgaccg
     1501 gggcgctgag cgccgcgagc tacgcggttg gccgctgaac gtgacgatct cgaaatcagc
     1561 gggcgtcagc ggctcgtccg ccgtccgctc cggcgttctc cgacgtcggc gcaaaaatcc
     1621 gccggcgttt cgcgctgatc ggccgcggtg atcgcgggcg ctccgtcgcc gtgcgcaacg
     1681 cgtggcggcg gatgccgagc cgcgcggttt cagccgcgca cgcgagctga aaatcctgca
     1741 tgcgttgaaa cagtccgagc agttgctccg gcagcggata gcgatcgagg cccgatcgct
     1801 ccaacgcgag caacagctcg tgcgttgcct caagggtgga aagacagccg ggctccggct
     1861 gctgcttgat cacgtagcgg ctcggcgcgg cattcgaaaa catgatgcgc ggcagccgtt
     1921 gcagcgtcgc actttcccgc cacatgcggc gcaccagccg ccaggtcgcg tccaacaaaa
     1981 acaccaccag ccgccgacct tcgaaatccg ccgcgtgcaa ctcgcctttc gaaagatcgc
     2041 gcgcgtcgcg cgtcggataa agcagcacgg gataattctc gggatcgttg atcaacgcct
     2101 gcaccgcctc gtgttggtcg aacccgatcc cgacgtgaat cgcactttcg gcgagacaca
     2161 gatgcgtaag ccgtcccgtc gcggccttca cgcgcttgaa ttcatacggg tgcatgagga
     2221 acacgaactt ggtgcgcgtc ggcatcggtg tgatcgacgc acaccaacac tgggccttgg
     2281 gccagaaaca ccggtaacac atcggtcgac tcatgagtcc ccgacgattg ccgcccggcc
     2341 tgcgcctgac cagcggcaaa atagaacgag caaaactaaa aggcgcagcc gatccggctc
     2401 gaaggcagga gcctgcttgc aggcgattgc gcgcgacgag cacgggcgat gcagcatatc
     2461 gcctgcgagc aggcttctgc aatcgcgacg aattatccag ccgcctcgtc gctagtccgc
     2521 cccgcgacat ccgccgcctc gccgactaca gctccgctgc cccgatcgtc agcgtgcgcg
     2581 cgtgctcgca gcgcttgcgc gatttctcca cgccgagcgc atcgagcccc atcgcgttcg
     2641 ccaccgcgag cacggtgccc acgccgcaaa acggatcgaa caccaaccgc gaacccacct
     2701 gatcgcgcgc gaaccgcacc gcatgcgcgg ccgcacgaat ccccatcgcg cgcacccacg
     2761 gctgccggcc cgcatcgaca atgatatccg gaatcgccaa ctcgtccgga caacgcatcg
     2821 ctcgcgacac cgtgatcaaa tgcgtgaaac ccggccgtcc cagcgtcagc aaccccggcg
     2881 gccgccgcgc cacgatcttg tgaaacaaca cccgcgcgcc cgcgtcctcc gccgcgcgca
     2941 ccaccatcgc gcctttgtcc acccacgcgc catcgcgctt gatgtcgctc tgaaaaaaca
     3001 ccgccgcact ctcgtcgggc accgcatcga tcacccgacg caccgcatcc aaaaaccaac
     3061 tacgccactc tggcagcgca aatcccactt cggagacgtc cggcagcga
//
LOCUS       DASPLT010000328         1288 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_270084, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000328 DASPLT010000000
VERSION     DASPLT010000328.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1288)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1288)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1288
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_270084"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1000
                     /gene="purH"
                     /locus_tag="VEA63_03445"
     CDS             <1..1000
                     /gene="purH"
                     /locus_tag="VEA63_03445"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HYD83072.1"
                     /translation="TAIARYLEAQAATPDLGALSGFPETFSVSWKKAQTLRYGENPHQ
                     QAALYGTFHEHFEQLQGKELSYNNILDITSATYLIGEFERPTVAILKHTNPCGVASAD
                     TLNDAWERAYATDRQAPFGGIIIVNQTLDGVLAKAIAEIFTEVIIAPRFSDEALAIFG
                     KKKNLRLMIAQGGIGADALLEVRSVVGGVLVQDRDRTLGNVSEFKVVTKRQPTPEEWA
                     GMMFGWKVGKHVKSNSIVYCRGEQTLGVGAGQMARVDSSRIAVWKAGEAKLDLKGSVV
                     ASEALFPFADGLIAAAEAGATAAIQPGGSVRDAEVIAAADERGMAMVFTGVRHFKH"
BASE COUNT          246 a          343 c          463 g          236 t
ORIGIN      
        1 cacggcgatc gcgcgttatc tcgaggcgca ggcggcgacg ccggacttgg gcgcgttgag
       61 cggatttccc gagacgtttt cggtttcgtg gaagaaggcg caaacgctgc gctacggcga
      121 gaatccgcat cagcaagcgg cgctttacgg gacgtttcac gagcacttcg agcaactgca
      181 agggaaggag ctgagctata acaatatttt ggatatcacg tcggcgacgt atctgatcgg
      241 cgaattcgag cggccgacgg tcgcgatttt gaagcacacg aatccgtgcg gcgtcgcgag
      301 tgcggacacg ttgaacgacg cgtgggagag agcttacgcg acggaccggc aggcgccgtt
      361 cggcgggatc atcatcgtca accagacgct cgatggcgtg ctggcaaagg cgatcgcaga
      421 gatcttcacg gaggtgatca tcgcgccccg gttctcggac gaggcgctcg cgatctttgg
      481 gaaaaagaaa aatctccggt tgatgatcgc gcaaggcggc atcggcgcgg atgcgttgtt
      541 ggaggttcgt tcggtcgtcg gtggcgtgct cgtgcaggat cgcgaccgca cgctcggcaa
      601 cgtgagcgaa ttcaaagttg tcaccaagcg gcagcccacg ccggaggagt gggcgggcat
      661 gatgtttggc tggaaagtcg gcaagcacgt gaagtcgaac tccatcgttt actgccgcgg
      721 tgaacagacg ctcggcgtcg gtgcgggcca gatggcgcgc gtggatagct cgcgcatcgc
      781 ggtctggaaa gcgggcgagg cgaagttgga tctgaagggc tcggtcgtcg cgagcgaggc
      841 gctgtttccg tttgcggacg gcttgatcgc cgccgcagaa gccggcgcaa ccgcagcgat
      901 ccagcctggc ggttcggtgc gcgatgcgga agtcatcgct gcggcggacg aacgcgggat
      961 ggcgatggtg ttcaccggag tgcggcattt caaacactga gcagccgagc ggagctgggc
     1021 gcgaaagcgt gacgagcggg gttgtcccga cccgcctatc aggctgcgcc ttgggcacgg
     1081 ccgcagtcct tgcggggcgc acggatctgg ctagcggttg cgcatgaaaa atttcctgcg
     1141 cgcgacactc gtaaccggat ttttggtgct cagtggttgt tacaccgtgc ccgagacagg
     1201 acgccaggcg gtgatcctgc cgattttcga cgacgtgcag atgggggcgc aggcgtttgc
     1261 cgagattcgg gcgaaagaaa aggtctcg
//
LOCUS       DASPLT010000329         2103 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_188304, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000329 DASPLT010000000
VERSION     DASPLT010000329.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 2103)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2103)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2103
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_188304"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(37..312)
                     /gene="rpsP"
                     /locus_tag="VEA63_03450"
     CDS             complement(37..312)
                     /gene="rpsP"
                     /locus_tag="VEA63_03450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HYD83073.1"
                     /translation="MALKIRLTKVGSVHQPLYRVVVAEARSRRDGDAVENLGTYTPKT
                     KGNPINLKLDRVDYWLSKGALPTNTMHAMIKKARRTAAATAEAPAAS"
     gene            566..1927
                     /locus_tag="VEA63_03455"
     CDS             566..1927
                     /locus_tag="VEA63_03455"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HYD83074.1"
                     /translation="MTKIETHRSRVPWSWVVWMTGPWLALYYIDNVNGGPLTFTIRKF
                     IENPALIGFLTSLNVAFNFLIGVAASYMSDRIWTRWGRRRPFLIVGWTGVAIAMACVP
                     FAPNVWVLGAIVVAYQFFADIAKPVEPLHNEVIPPGQRGRAASVQNIGQQLLGLVFFG
                     VLLAQFDHVHEFTLFGHDLHLAGETVLYWCGSALLFCVVLFLAFRLRETPPPERITRE
                     PFALRRFFREVFGQRQWWMIYLLYVTPMIAAPTGNFHMLLRTDQLGFTKEQLGYAITF
                     GLIVMILVFAPLGGYLADRMSRMRLLRIGLIGPAIVEFVFFLHLRYVAHYSISLSTLI
                     IYGTIAGAMATCMYIVWGPLTYDYIPSERYGTVAAGLTFVGGLVPFLTINLTGLWITG
                     FTKIFGPIGGGHYDYSSMFLFQLAGAALALGLTYYFEREERRGRIRPFGRLERAAESA
                     TKM"
BASE COUNT          354 a          694 c          603 g          452 t
ORIGIN      
        1 cagaggcgct gcgcctccgg gtctgtgcgg ttcaaatcag ctcgcggcgg gagcttcggc
       61 cgtggcggcc gcggtgcggc gggccttctt gatcatcgcg tgcatcgtgt tcgtcggcag
      121 cgcgcctttg ctcaaccagt agtccacgcg gtcgagcttg agattgatcg ggttaccctt
      181 ggtcttgggc gtgtaggtgc cgaggttctc gacggcatcg ccatcgcgac gcgaacgggc
      241 ttcggcaacc acgacgcgat agagcggctg atgcacggag ccaaccttgg taagacgaat
      301 tttgagagcc atggcagatg ttttgcggag ctattcttgc ttggaaaagc tgaggacaag
      361 acctttccac cttccgcttg tcaagcccct agccggacgc ctgcaacgaa cgcgaaaaat
      421 accggcgcat ctcggacaag aactcgatcg cgctgcccgc gcaccgccgt cgaatctgcg
      481 gcacgcggtg catcgcatga caccgtttcc ccaagccacg ggttgcaccg tcgcgaggcc
      541 gccggttcaa tgaatttcgc ccccgatgac gaagatcgaa acccaccgct cgcgtgtgcc
      601 ttggagctgg gtggtctgga tgaccggccc gtggctggcg ctctactaca tcgacaacgt
      661 caacggcggt ccgctcacgt tcacgattcg aaagttcatc gagaatcccg ctctcatcgg
      721 atttctcacc agcctcaacg tcgcgttcaa cttcctcatc ggcgtcgccg ccagctacat
      781 gagcgatcgc atttggacgc gctggggacg ccgccgtccc tttctgatcg tcgggtggac
      841 cggcgtggcc atcgcgatgg cctgcgtgcc ctttgcgccg aacgtctggg tgctcggcgc
      901 gatcgtggtc gcgtatcagt tttttgcgga catcgccaaa cccgtcgagc cgctccacaa
      961 cgaggtcatc ccgccgggcc agcgtggacg cgccgcgtcg gtgcaaaaca tcggccagca
     1021 actactcggc ctcgtgttct tcggcgtgct gctggcgcaa ttcgaccacg tgcacgagtt
     1081 cacccttttc ggccacgacc ttcatctcgc cggcgaaacg gtgctctatt ggtgcggcag
     1141 cgcgctgctt ttctgcgtcg tgcttttcct cgcctttcgg ctgcgcgaaa ctccgccacc
     1201 cgaacgcatc acgcgtgaac catttgcgct gcgcagattt ttccgggagg tgttcggcca
     1261 gcgccagtgg tggatgatct acctgctcta cgtgacgccg atgatcgccg cgccgacggg
     1321 aaattttcac atgctcctcc gcaccgacca actcgggttc accaaggagc aactcggtta
     1381 cgcgatcacg ttcggcctga tcgtcatgat cctggtcttc gccccgctcg gcggatatct
     1441 cgcggaccgc atgtcgcgca tgcgcctgct ccgcatcggc ctgatcggac cagcgatcgt
     1501 cgagttcgtt ttcttcctgc atctccgcta cgtcgctcac tactcgatct cgctgtcgac
     1561 gctcatcatc tacggcacca tcgccggcgc gatggccacg tgcatgtata tcgtttgggg
     1621 acctctgacc tacgattaca tcccgagcga acgctacggc accgtcgccg cgggactcac
     1681 gtttgtcggc ggcctcgtgc ctttcctcac gatcaatctc accggtcttt ggatcacggg
     1741 cttcaccaaa attttcggtc cgatcggcgg cggacactac gactattcca gcatgttcct
     1801 ctttcagctc gccggcgctg cccttgcact cggcctcacc tactatttcg aacgcgagga
     1861 acgccgcggc cgcatccgtc cgttcggccg gctcgagcgc gccgccgagt ccgccacaaa
     1921 gatgtgaccg ctgtccggac atgatgtctt cctgcgcgca ggctcgaatc aaagcctttg
     1981 aacaaaaggc cgcgaaggga acaaaggcgc tggaggataa tgactcttcg ttccattcgt
     2041 ggccttctgt tcaaaaatcg gcgctcgagt tcggcggcag cgcgcgcccg gattcgctcg
     2101 tgg
//
LOCUS       DASPLT010000330         3116 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_4387, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000330 DASPLT010000000
VERSION     DASPLT010000330.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 3116)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3116)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3116
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4387"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..321
                     /locus_tag="VEA63_03460"
     CDS             <1..321
                     /locus_tag="VEA63_03460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD83075.1"
                     /translation="MAAQSVTTRINELSSKRLLPLEMITFIVEKKPADVRLLRATTVG
                     LYGLSIDGSTQSPAAVSTFQTALSSDPALERVEVPVNRARDNVTTFTFLVTFRPNTLV
                     PATQ"
     gene            318..848
                     /locus_tag="VEA63_03465"
     CDS             318..848
                     /locus_tag="VEA63_03465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD83076.1"
                     /translation="MRALKAFFLSRLLREKVLLVLFVALGAALWGSNFTTRATADWRA
                     ARAVTTELALQKQWLDNRQTIEEASIQAVQNLDPARTLDDTRLVGDLSALAREHNLRF
                     TNDTPQTERSGEFAIHTVQVQLENAPWDALKPFYLALTARSPYLGIEQFSARPDRADP
                     SLLDTSIRISSVEIVR"
     gene            1017..2198
                     /locus_tag="VEA63_03470"
     CDS             1017..2198
                     /locus_tag="VEA63_03470"
                     /inference="COORDINATES: protein motif:HMM:NF023516.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3570 domain-containing protein"
                     /protein_id="HYD83077.1"
                     /translation="MFRSTLLPLSPFRSLDRSAVLLMAACFFGQARAARAEGAISYKY
                     HDYREADDRITVESHYGMIEAALGPAMRFKAHGVIDAIAGATPTGEPPATPDGPVPLS
                     EIEDRRKGWSAEVSRQFPRVNAAVGVANSRESDYVSTGWSLNTTTDFNQKNTTLLLGV
                     AGTDDDIKVFYQTDWVKKRTTDLVAGVTQLLNPNTAVTVNVGYGHSSGFHADPYRLIF
                     KRVEIAPGVFLPRTFNENRPDSRDKWTMFAALNRAFPQANGALEASYRLFRDDFGITA
                     HTLELAWFQKFGGRFTLRPAIRLYEQTEADFYRLDLTGTDITPTNGPNPVGPFFSADY
                     RVSAFWSHTLGLKGVWTVSDAWQVDATLEHYEMRGKDNATSPHVYPRAAIVTIGARFG
                     W"
     gene            2205..>3116
                     /locus_tag="VEA63_03475"
     CDS             2205..>3116
                     /locus_tag="VEA63_03475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HYD83078.1"
                     /translation="MALVAHRLPSPTPDLSCGSSGLRRLSFSALGTVCDVQYACDQPA
                     VAQTFERHVVQWVQAFEAKYSRFREDSLVSRINAAAGRGWVEIDAEVEQLFKLCDTLV
                     FLTQGIIDPTILPLMRLWNYKVPRTVLPAEAEIARARSLVGWRKVKREPGRVFLPEPG
                     MALDFGGFGKEYAVDVVAQIAIDHGIANVLVDFGHDLRALGRPPDRPAWHIGLENPSM
                     PGVAAGSVALTGNRGIASSGDYLRGFALGGRRYGHIVDPRTGWPVANGCMQATAIASS
                     CLQAGVLSTTAFVLGVAKGIEFIQAAPG"
BASE COUNT          557 a          940 c         1015 g          604 t
ORIGIN      
        1 atggcggcgc agagcgtcac gacgcgcatc aacgagctct cgtcgaaacg gctgctgcca
       61 ctggagatga tcacgtttat cgtggagaag aaacccgcgg acgtgcggtt gttgcgcgca
      121 acgacggtgg ggctctacgg cttgagcatc gacggcagca cgcaatcgcc ggcagcggta
      181 tcgaccttcc aaaccgcgtt gagtagcgat ccggcgctcg aacgcgtgga ggtgcccgta
      241 aaccgtgccc gcgataatgt gaccaccttc acctttctgg tgaccttccg tcccaacacc
      301 ctcgtccccg ccacccaatg agagcgctca aagctttttt tctcagccgg ttgctccgcg
      361 agaaagtgct gctcgtgctg ttcgtcgcgc taggtgcggc gttgtggggt tcgaatttca
      421 ccacgcgggc gacggctgac tggcgggcgg cgcgagcggt gaccaccgaa ctggcgctgc
      481 agaagcagtg gctggataat cggcagacga tcgaagaagc atccatccaa gcggtgcaga
      541 atctcgaccc ggcgcggacc ctcgacgata cgcggctggt cggcgatctg agcgcgctcg
      601 cgcgcgagca caatttgcgt ttcaccaacg atacgcccca aaccgagcgc agcggagagt
      661 tcgcgattca cacggtgcag gtccagcttg agaacgctcc gtgggatgcg ttaaagccct
      721 tctatttggc cctgacggca cgctcgccct acttgggcat cgagcagttc tcggcgcgtc
      781 cggaccgcgc agatcccagc ctgctcgata cgtcgatccg catttcttcg gtcgagatcg
      841 ttcgataacc ggagcgcgac gcggcggttt tgcaggcggt cggctgagcg accggcatcg
      901 acgcggtaaa gttgcttgga tcaggtgcgt gctttggagc ggaggccaat ctttccaaaa
      961 cgttacggtt aaccggttgg acgagcctcg cgcggcaacg aggttggatc acagcgatgt
     1021 ttcgatccac gctccttccg ctctcgccct ttcgttcgct cgaccgcagc gcggtcctgc
     1081 tgatggcggc gtgttttttt ggccaggcgc gtgccgctcg cgccgagggc gcgatcagct
     1141 acaagtatca cgactatcgc gaggcggacg atcgtatcac cgtcgaatct cactacggca
     1201 tgatcgaagc ggcgctcggt ccggcgatgc gcttcaaggc gcacggcgtg atcgatgcga
     1261 tcgccggcgc cactccgacc ggtgaaccgc cggcaacacc ggacgggccg gtgccgttgt
     1321 ccgaaatcga agatcggcgg aaaggctgga gcgctgaggt gtcgcggcaa ttcccccgcg
     1381 tcaatgccgc ggtcggcgtc gccaatagtc gggagagcga ctacgtgtcg accggctggt
     1441 cgctcaacac gaccacggat ttcaaccaga agaacacgac gctgctgctc ggcgtcgccg
     1501 gcacggatga cgacatcaag gtgttctacc aaaccgattg ggtgaagaag cgcacgaccg
     1561 atttagtcgc gggcgtcacg cagcttctca atccgaacac ggccgtgacc gtgaacgttg
     1621 gctacgggca cagctcggga tttcacgccg acccttatcg gctgattttc aaacgcgtgg
     1681 aaatcgcgcc cggcgttttt ctgccgcgaa cttttaacga gaatcgtccg gactcgcgcg
     1741 acaaatggac gatgttcgct gcgttgaacc gcgcttttcc gcaggcgaac ggcgcgctcg
     1801 aagcgagcta tcgattgttt cgcgacgatt ttggaatcac cgcgcacacg ctggagctcg
     1861 cgtggtttca aaagttcggc gggcgtttca cgctccgacc ggcgatccga ctctacgagc
     1921 aaaccgaagc ggacttttac cggctcgatc tcacgggcac agacatcacg cccacgaatg
     1981 gcccaaaccc ggtggggccg tttttttccg ccgattatcg cgtatccgca ttttggagtc
     2041 acacgttggg actcaagggc gtgtggacgg tcagcgacgc gtggcaggtc gacgcgacgc
     2101 ttgagcatta cgagatgcgc gggaaggaca acgcgacctc gccgcacgtt tatccccgcg
     2161 cggcgatcgt cacgatcggc gcaaggttcg gctggtaatt ctccatggct ctcgtcgctc
     2221 atcgcctgcc ttcgccaacg ccggatcttt cctgcgggtc gagtgggttg cgccggctgt
     2281 cgttttccgc gcttgggacg gtgtgcgacg tccaatatgc gtgtgaccag ccggcggtcg
     2341 cgcagacgtt cgagcggcac gtggtgcaat gggtgcaggc attcgaggcg aagtattcgc
     2401 gtttccgcga agacagtcta gtgagtcgca tcaatgccgc ggcaggtcgc gggtgggtcg
     2461 agatcgacgc ggaagtggag cagttgttta aactctgcga cacgctcgtc tttctcacgc
     2521 aggggatcat cgatccgacg attctgccgt tgatgcggct ctggaattac aaggtgccgc
     2581 gcaccgtatt gccggcggag gcggagatcg cacgcgcgcg gagcctcgtc ggttggcgga
     2641 aagtgaaacg tgaacccgga cgcgttttcc tgcccgaacc gggaatggcg ctcgacttcg
     2701 gcggcttcgg caaggaatac gcggtggatg tcgtagctca gatcgcgatc gatcacggga
     2761 tcgcgaatgt gttggtggat ttcggacacg acttgcgcgc gctcggccgt ccgcccgacc
     2821 gaccggcgtg gcacatcggg ctggagaacc cgagcatgcc cggcgtcgct gctgggagtg
     2881 tggcgctgac gggaaatcgc ggcattgcgt cgtcgggcga ttatctgcgc ggcttcgccc
     2941 tcggtgggcg gcgttacggc cacatcgtcg atccgcgcac gggctggccg gtggcgaatg
     3001 gctgcatgca ggcgacggcg atcgcctcga gctgcctgca ggccggcgtg ctgtcgacga
     3061 cggcgttcgt tctcggtgtg gccaaaggca tcgagttcat ccaggcggcg ccgggc
//
LOCUS       DASPLT010000331         1008 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_147587, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000331 DASPLT010000000
VERSION     DASPLT010000331.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1008)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1008)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1008
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_147587"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1008)
                     /locus_tag="VEA63_03480"
     CDS             complement(<1..>1008)
                     /locus_tag="VEA63_03480"
                     /inference="COORDINATES: protein motif:HMM:NF025214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD83079.1"
                     /translation="RTTDTVLEEYCRQRAADFFVLPDDVDGQLAMLRAAALDIVVFGT
                     NVTAIYNEVTQIALHRVAPLQVVNNSSCITSGLPEADLYVSGTLTETAGAEVNFSERL
                     ALMPGPAHAFNYEADREEPQMPCTRAEFGIPDEAFVFVSGSNYFKIIPEMQHAWAKLL
                     AAVPGSRLLLHPFNPNWSSSYPIKRFRAEFERVLASHGVEASRLAISTLRFPSRTDVK
                     GLLALGDVYLDSFPFGGVNSLIDPLELGIPVVVWDGKTMRSRMGAALLRQINLPELIA
                     DNDVGYHALAVKLAKDATLLETCRARIRERMERTPLFLDPLAASDAFGDVLEKAYDEL
                     AE"
BASE COUNT          212 a          352 c          268 g          176 t
ORIGIN      
        1 ttccgccagc tcatcgtaag ctttttccag cacatcgccg aacgcgtcgc tcgccgccaa
       61 cggatcgagg aagagcggcg tccgctccat tctttcgcga atgcgcgcac gacacgtttc
      121 gagcagggtc gcatccttgg ccagcttcac cgcgagcgcg tgatagccca cgtcgttgtc
      181 ggcgatcagc tccggcaaat tgatctgacg caagagcgcg gcacccattc gcgaccgcat
      241 cgttttgcca tcccacacca ccaccggaat tccgagttcc agcggatcga tcaacgaatt
      301 cacgccgccg aaaggaaagc tgtcgagata cacgtcaccg agagcaagca ggcctttgac
      361 gtccgtgcgc gacggaaacc gcagcgtcga gatcgccaac cgcgacgcct ccaccccatg
      421 actcgccaac acccgctcga actccgcgcg aaagcgcttg atcggataac tcgacgacca
      481 gttcggattg aacggatgta gcaacaaccg cgaacccggc acggccgcca gcagcttcgc
      541 ccacgcgtgc tgcatctccg ggatgatctt gaaataattc gaacccgaca cgaacacgaa
      601 cgcctcatcc ggaatcccga attccgcgcg cgtgcacggc atctgcggct cctcacgatc
      661 ggcttcgtag ttgaacgcgt gcgccggccc cggcatgagc gccagccgct cgctaaaatt
      721 gacctctgct cccgcggtct ccgtcagcgt gcctgaaacg taaaggtcgg cctccggcaa
      781 acccgacgtg atacacgatg agttgttcac gacctgaagc ggcgcgaccc ggtgcagtgc
      841 gatctgcgtc acttcgttgt agatcgccgt cacgttcgtg ccgaacacca cgatatcgag
      901 cgccgccgcc cgcaacatcg cgagttgccc gtcgacgtcg tccggcaaaa caaaaaagtc
      961 cgccgcacgc tgccggcagt attcttcgag caccgtgtcc gtcgttct
//
LOCUS       DASPLT010000332         2116 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_147594, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000332 DASPLT010000000
VERSION     DASPLT010000332.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 2116)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2116)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2116
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_147594"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..232)
                     /locus_tag="VEA63_03485"
     CDS             complement(<1..232)
                     /locus_tag="VEA63_03485"
                     /EC_number="1.-.-.-"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HYD83080.1"
                     /translation="MASSFLHLASTPAVVAAQLRYHGHAAVPGDPPPVDQLGPDEREF
                     IAARDSFYLATVTETGWPYVQHRGGPSGFLRVL"
     gene            complement(250..624)
                     /locus_tag="VEA63_03490"
     CDS             complement(250..624)
                     /locus_tag="VEA63_03490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD83081.1"
                     /translation="MKSAIVIMSDPKPGTDEALGRVLNALAFAHESRAAGDEVEIVFK
                     GAGTRWPGELAKLGHPAHERYQAVRESVKGASLGCAMKYGARNEVAASGVALLADTAF
                     PGTPGIAGLHAYYRDGWNVVVF"
     gene            771..1361
                     /locus_tag="VEA63_03495"
     CDS             771..1361
                     /locus_tag="VEA63_03495"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HYD83082.1"
                     /translation="MSAALTESPPPSPKRAHLLLTAWRLFYRDGYRAVGIDTLLAEAQ
                     VAKMTLYNHFASKEELIVAVLEQRSAALLKALDESLAAAGRTPQRRLTAVFDGLSQWV
                     EADDFKGCAFIRALSEYPDSSHPIHTAAWRHKRAMNARLRTIVEAAGAKNPTALADTI
                     SLLIDGAIVSAHATGSADSIDKARAAAISLLKLASA"
     gene            complement(1342..>2116)
                     /locus_tag="VEA63_03500"
     CDS             complement(1342..>2116)
                     /locus_tag="VEA63_03500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="zinc finger domain-containing protein"
                     /protein_id="HYD83083.1"
                     /translation="EGRKVAELRRIGKRIAFGFEGERWLVLHLMIAGRLQWTDTGGKA
                     VARNVLANFVFENGTLALTEAGTKRRAALHVVDGEQGLAAHDPGGIEVMTATLEEFGA
                     ALTKENHTVKRSLTDPHVFSGIGNAYSDEILHAARLSPVKLTQKLTADEIGRLQAATR
                     EVLRAWTERLSGEAKAKFPEKVTAFRPEMAVHGKFGKPCPVCGTAVQRIVYAENEVNY
                     CPRCQTGGKLLADRSLSRLLKQDWPRTLEESEALKRSRA"
BASE COUNT          383 a          791 c          564 g          378 t
ORIGIN      
        1 ccaacacgcg caaaaacccg ctcggcccgc cgcgatgttg cacgtaaggc caacccgttt
       61 ccgtgaccgt cgccaggtag aagctgtcgc gagcggcgat aaattcgcgc tcgtccggcc
      121 ccagctggtc gaccggcggc ggatcgcccg gaacggccgc atgcccgtga tagcgtagct
      181 gcgccgccac caccgcgggc gtgctcgcca aatgcagaaa actcgaggcc atggcgtgag
      241 cgggttcgcc tagaacacca caacgttcca cccatcgcgg taataggcgt gtaatcccgc
      301 gattcccggc gtgcctggaa acgctgtgtc cgccagcagc gcgacaccac tcgccgccac
      361 ctcgttgcgc gcaccatact tcatcgcgca acccagcgag gcccctttca ccgactcgcg
      421 caccgcttga taacgttcat gcgcgggatg acccagcttc gccagttcgc ccggccagcg
      481 cgtgccagcc cccttgaaca cgatttccac ttcgtcgcca gctgcacgac tctcatgcgc
      541 aaacgccagg gcattcaata cccgcccgag cgcttcgtcg gtgccaggct tcggatcgga
      601 cataatcaca attgcagatt tcatttttcg attcgttgga ggtttccgtc gctggatggc
      661 gaacatcgcc aggccgacac tagacagacc tgtctagtag agagcaagag aaaagagaca
      721 ggtctgtcta ttcaccttgc gcgccgcggc gccaacagca cactgacgcc atgtccgccg
      781 cgttgaccga aagcccgcct ccgtcgccca agcgcgcgca cttgctcttg acggcctggc
      841 ggctgtttta ccgcgatggc tatcgcgccg tcggcatcga cacgctgctc gccgaagcgc
      901 aggtcgcgaa gatgacgctc tacaaccatt tcgcgtcgaa agaagagctg atcgttgccg
      961 tgctcgagca gcgcagcgcc gccctgctga aggcgctcga cgagtcactc gccgccgccg
     1021 gtcgcacccc acagcgccgg ctcaccgccg tcttcgacgg gctgagccaa tgggtcgagg
     1081 cggacgattt caaaggctgc gccttcatcc gcgccctgag cgagtatccg gattcttccc
     1141 atccgattca cacggccgcg tggcgccaca agcgcgcgat gaacgcccgc ctccgcacca
     1201 tcgtcgaggc cgccggcgcg aaaaatccca ccgcgctggc cgacacgatt agcctgctga
     1261 tcgatggcgc catcgtatcg gcgcacgcga ccggttccgc cgactcgatc gacaaagcgc
     1321 gcgcggccgc gatcagcctc ctcaagctcg cgagcgcttg agcgcctcgg attcctcgag
     1381 cgttctcggc cagtcctgtt ttaacagccg cgacagcgaa cgatccgcga gcagctttcc
     1441 accggtctgg cagcgcggac aatagttcac ctcgttctcc gcatacacga tccgctgcac
     1501 cgcggtcccg cacaccgggc acggtttgcc aaacttccca tgcaccgcca tctccggccg
     1561 aaacgccgtc accttttccg gaaactttgc cttcgcctcg ccgctcaacc gttccgtcca
     1621 cgcgcgcaac acctcgcgcg tcgctgcctg caggcgcccg atctcgtccg ccgtcagctt
     1681 ctgcgtgagc ttcaccggcg acaatcgcgc cgcatgcaga atctcatcgg aatacgcatt
     1741 cccgattccg ctgaacacgt gcggatccgt cagcgaccgt ttcaccgtgt gattctcctt
     1801 cgttagcgcc gctccaaatt cttccagcgt cgcggtcatc acctcgatcc cgccgggatc
     1861 gtgcgccgcc agcccctgtt cgccgtcaac gacgtgcaac gccgcgcgcc gcttcgtgcc
     1921 cgcctcggtc aacgccagcg ttccgttttc aaaaacaaaa ttcgcgagca cgttccgagc
     1981 gaccgccttg ccccccgtat ccgtccactg gagacgccct gcgatcatca aatgcagcac
     2041 gagccaccgc tcgccctcga acccgaacgc gatccgtttt ccaattcttc gcagctccgc
     2101 caccttccgc ccttcg
//
LOCUS       DASPLT010000333         1029 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_86450, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000333 DASPLT010000000
VERSION     DASPLT010000333.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1029)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1029)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1029
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_86450"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..858
                     /locus_tag="VEA63_03505"
     CDS             <1..858
                     /locus_tag="VEA63_03505"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HYD83084.1"
                     /translation="LEEAINGNTGRDNTGLYLEYISDFSDALHLTAGVRHDDNDDFGT
                     NTSYRVSGAYLFDLASGDTLKFRSSYGTGFRAPSPYEIAYNSGAFASPPASLVTLQQE
                     TSKGYEFGIEYLADAVRLEAVYFNQDVEDAIFFDLAAFSGYLQDVGLSTSKGVELGGE
                     WTLGQWQLSANYTHNDTALPNGEPRRRRPENLTNFGLSYFAMDERLNLNAYWRIVRDS
                     FDQVGATIVPLDDFDVLDLTANFSITGSMQIYGRLENALDEDYREVAGFNTAGRAAYV
                     GFRLNYAGL"
BASE COUNT          206 a          352 c          288 g          183 t
ORIGIN      
        1 ctcgaagaag ccatcaacgg caacaccggc cgcgacaaca ccggccttta cctcgaatac
       61 atcagcgact tctccgacgc actgcacctg accgccggcg tgcgccacga cgacaacgac
      121 gacttcggca cgaacaccag ctaccgcgtc agcggcgcct acctgttcga tctcgccagc
      181 ggcgatacgc tgaaattccg cagcagctac ggcaccggct tccgcgcgcc gagtccgtac
      241 gagattgcct acaactcggg tgcgttcgca tcgccgcccg cgtcgctcgt cacactgcaa
      301 caggaaacca gcaagggtta cgaattcggc atcgaatacc tcgccgacgc agtgcgcctc
      361 gaagcggtgt atttcaacca ggacgtcgaa gacgcgatct tcttcgacct cgccgcgttc
      421 agcggttacc tgcaggacgt cggcctcagc acgtcgaaag gcgtcgaact cggtggcgag
      481 tggacgctcg gccagtggca gctcagtgcg aactacacgc acaacgacac cgcgctgccg
      541 aatggcgagc cgcgccgtcg ccggcctgaa aacctgacga atttcggcct ttcgtatttc
      601 gcgatggacg agcgactgaa cctgaatgcg tactggcgca tcgtgcgcga ctcgttcgac
      661 caggtgggtg cgaccatcgt gccgctcgac gacttcgacg tgctcgacct gacggcaaat
      721 ttcagcatca ccggcagcat gcagatttac ggccgactcg aaaacgcgct cgacgaggat
      781 taccgtgaag tcgccggttt caacactgcc ggacgcgcgg cctacgtcgg ctttcgcctg
      841 aactacgcag gactgtaacg cgtaaggcca gcgcttttcc cttcaatgcc gcaggcggtt
      901 agactgcggc atcatgtcac gcgttcacag tttcccgcct cttgccgcgc ccgatgccac
      961 tcgcctgatc ctcggttcga tgccaggcgt gctgtcgctc gaagcacggc agtattacgc
     1021 gcacccgcg
//
LOCUS       DASPLT010000334         1183 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_65246, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000334 DASPLT010000000
VERSION     DASPLT010000334.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1183)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1183)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1183
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_65246"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            97..>1183
                     /locus_tag="VEA63_03510"
     CDS             97..>1183
                     /locus_tag="VEA63_03510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD83085.1"
                     /translation="MLIDQKLSVRLAALNDESAFEQRPAYRDQARYYGAIEFRPFRRT
                     TIRVNAETGEVNQSLPRIDPPMDALSAWFAFGKPERANNLYPGPPFTAAGNPRIADDV
                     AKNLNGPYGTFFYHPGVFFGDDSGNPTDAFPSFADTPTLQYRYLAPANSLVVETLQGS
                     VLSSFKVPNQIIDRSIFDYRKRKIDGLNDGTFVDFDALNVAVEQLFLDGDAGLEFVYD
                     RQEVSTDIIEINQQPRGNEIRIDPNRQTVDGRPNPNFGRPFISSIGQSSRSVNEDESV
                     RFTGFYKADFTQRIDNWVGRLLGTQTLTGFYTDSTTKNYSIGAANALIAPTFVSGANT
                     SGIGDRRIATIYYLGPSLAGAASPAGAN"
BASE COUNT          227 a          410 c          326 g          220 t
ORIGIN      
        1 aacacgacca ttgcgccact ttttcgcaat cacggtaagg tcgagctgcg tttcgacagc
       61 aaccacagca cgcgtcaggc gctcgacatc gagcgcgtgc tcatcgatca aaaactctcc
      121 gtgcgcctcg cggcgctgaa cgacgagagc gcttttgagc agcgtccggc gtatcgcgac
      181 caggcgcgtt actacggggc catcgagttc cggccgtttc gccgcacgac gatccgcgtg
      241 aacgccgaga ccggcgaggt caaccagtcg ctgccacgca tcgatccccc gatggacgcg
      301 ctgtccgcgt ggttcgcttt cggcaagccg gagcgcgcca acaatcttta tcccggcccg
      361 cccttcaccg cagccggcaa tccccgcatc gccgatgacg tcgcgaagaa tctcaacggc
      421 ccgtatggca ccttctttta tcatcccggt gttttcttcg gcgatgacag cggcaatccg
      481 accgatgcct tcccctcgtt cgcggacacg ccgacgctgc aataccgcta tcttgcgccg
      541 gcgaacagcc tcgtcgtgga gacgctccag ggtagcgtct tgagctcctt caaggtgccg
      601 aatcagatca tcgaccgttc catcttcgat tatcgcaagc gcaagatcga tggcctgaac
      661 gacggcacgt ttgtcgattt cgacgcgctc aatgtcgcgg tggaacagct cttcctcgac
      721 ggcgatgccg gcctcgagtt cgtctacgac cggcaggagg tctccaccga tattatcgag
      781 atcaaccagc agccgcgcgg caacgaaatc cgcattgatc cgaaccgcca gacggtcgac
      841 ggccgcccga atccgaactt cggtcgtccc ttcatctcgt ccatcggcca gtccagccgt
      901 tcggtgaacg aagatgaaag cgtgcgcttc accggtttct acaaagccga tttcactcag
      961 cgcatcgaca actgggtcgg ccggctgctc ggcacgcaga cgctgacggg tttctacacc
     1021 gattccacga cgaagaacta cagcatcggc gccgccaacg cgttgatcgc gccgaccttc
     1081 gtgagcggtg ccaacaccag cggcatcggc gatcgccgca tcgcgacgat ctattatctc
     1141 gggccctcgc tcgccggcgc cgcgagcccc gcgggcgcga acc
//
LOCUS       DASPLT010000335         1129 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_147640, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000335 DASPLT010000000
VERSION     DASPLT010000335.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1129)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1129)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1129
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_147640"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..425
                     /locus_tag="VEA63_03515"
     CDS             <1..425
                     /locus_tag="VEA63_03515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD83086.1"
                     /translation="GFVDVTSVRPNAGKSISPVKLLGSVVYREEGFFPLRTGDPQRGV
                     AFVFKGYRLLGSAIEFRYEIDHRLVIEEVAPLPDGLGLVRRFHIEGAAAGEPWWYVPG
                     ANSGGKLTALQAVQDAGGFRFDAAREFALEVHFEKEAR"
     gene            422..>1129
                     /locus_tag="VEA63_03520"
     CDS             422..>1129
                     /locus_tag="VEA63_03520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD83087.1"
                     /translation="MSRLAWMLLLCAVTLRAKAAYHVETVVLPTELRGGISGVAVTPK
                     GTLVLATRYGEIWMRDADTAPWRKFAGGLDEPLGLIAESEDVVYVAHRPELLKLSDTN
                     GDRRADTFDVLGYDWGQSNNYHEFFYGLRRDAAGNFYGAISLDSSGAKEPPGRTRGVR
                     NITPAVEASYHNSEMPYRGWAVKITPAGETIHFASGLRQANGVGMSPSGDLFMTDNQG
                     DYKPSCGLMHVEFNDFHG"
BASE COUNT          204 a          318 c          392 g          215 t
ORIGIN      
        1 gcggtttcgt cgacgtcacg agcgtccggc cgaacgcagg caaatcgatc tcgccggtga
       61 agctgctggg aagcgtggtc tatcgagaag agggattttt cccgctccga acgggcgatc
      121 cgcagcgagg ggtggcgttc gttttcaaag gctaccgcct gctggggagc gcgatcgagt
      181 ttcggtatga gatcgatcat cgactcgtta tcgaggaggt ggcgccgctg cccgatggct
      241 tgggtctggt gcggcgtttc cacatcgaag gcgcggcggc gggcgagcca tggtggtatg
      301 tgccgggcgc gaattccggc ggaaaactca ccgcgttgca ggcagtgcag gatgcgggag
      361 gatttcgctt cgacgctgcg cgggagttcg cgctcgaagt gcatttcgaa aaggaggcgc
      421 gatgagccgg ctcgcttgga tgctgcttct gtgcgccgtc acgctccgcg caaaggcggc
      481 ctaccacgtc gaaaccgtcg tgctgccaac ggaactacgc ggcggaattt ccggcgtcgc
      541 cgtcacgccg aagggaacgc tggtgctcgc gacgcgttat ggagaaattt ggatgcgcga
      601 cgccgacacg gcgccgtggc ggaaattcgc gggcggactc gatgagccgc ttggattgat
      661 cgctgagtcg gaggacgtcg tttacgttgc gcaccggccg gagctgttga aattatcgga
      721 tacgaacggc gaccggcgcg cggacacgtt cgatgtgctc ggttatgact ggggccagtc
      781 gaacaattac cacgagttct tctacggatt gcgacgggat gcggccggaa atttctacgg
      841 tgcgatctcg ctcgactcct cgggcgcgaa ggagccgccg ggacggacgc gcggcgtgcg
      901 caacatcacg ccggcggtcg aagcttccta ccacaactcg gaaatgcctt atcgcggctg
      961 ggcggtgaag atcacgcccg ccggcgagac gattcatttc gcctccggtc tgcggcaggc
     1021 gaacggcgtc gggatgagcc cttcgggcga tctgttcatg accgacaacc aaggcgacta
     1081 caaaccctcg tgcgggttga tgcacgtcga gttcaacgat tttcacggc
//
LOCUS       DASPLT010000336         1948 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_270174, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000336 DASPLT010000000
VERSION     DASPLT010000336.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1948)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1948)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1948
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_270174"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(107..1303)
                     /locus_tag="VEA63_03525"
     CDS             complement(107..1303)
                     /locus_tag="VEA63_03525"
                     /inference="COORDINATES: protein motif:HMM:NF019911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HYD83088.1"
                     /translation="MKFLRLCLVFAGIAAATCLVRAQDAQPSSTVEKRTYEKDGKTVT
                     ERVITTRVTDVAVKPSVYTAAIFVSNRAGKEHDDKIASLEDYITSGVTDKGVSVISRE
                     TALNAVQGLDADAKANALDQELAQSSSAVRLAQTLGADYLLQVSLSSVGSKKNSVNAY
                     GVKTTNNEQTVRVSYKILDGTTGASLTADTVRASRMVQGTANVSEDSSDVLNELLDEA
                     AGKVAASLQSRIERGRIAAPAAGGGLVNVTIAVEVADLVIPDVRIGVENTVAISDSKF
                     KVAPMGTTVEVDGLAIGSAPGTFEVKPGLHKLRVTREGFQPWERTVNFTRDMTLNVAI
                     QMSAAGFARWQEATQFLNDLKNGAKLTDAEVRRLEGEAKMLEQSGFRVDTKEGVTIKR
                     NSIFGD"
     gene            1603..>1948
                     /locus_tag="VEA63_03530"
     CDS             1603..>1948
                     /locus_tag="VEA63_03530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HYD83089.1"
                     /translation="MSAAYLLALDQGTTSSRAIVFDRRGRSRATAQQEFPQHFPRPGW
                     VEHSPRDLWETTRRVALAAVGEANLTARNLAAVGLTNQRETTLLWDRRTGEPLAPAIV
                     WQDRRTAALCAQL"
BASE COUNT          337 a          708 c          547 g          356 t
ORIGIN      
        1 gcgaacaaac tcgcggcgag cgcgaaaaca aaaatgcggg aaaggttcat gtcagggatg
       61 gcttgcagtt gagtggaagt gatgcggtcg ctgccgagct cacggctcag tcgccgaaga
      121 tcgagttgcg cttgatcgtg acgccttcct tcgtgtccac gcgaaatccg ctttgctcga
      181 gcatcttcgc ttcgccttca agccggcgca cttcggcgtc ggtcaacttc gcgccgttct
      241 tcaaatcgtt caggaactgc gtggcttcct gccagcgcgc aaaacccgcc gcgctcatct
      301 ggatcgcgac attcaacgtc atgtcgcgcg tgaaattcac cgtgcgctcc cacggctgaa
      361 aaccttcgcg cgtcacgcgc agcttgtgca gccccggctt cacttcgaac gtccccggcg
      421 ccgaaccgat cgcgaggccg tcgacttcca ccgtcgtgcc catcggcgcc accttgaatt
      481 tgctgtcgga aatcgcgacc gtattctcaa cgccgatacg cacgtccgga attacgagat
      541 ccgcgacttc caccgcgatc gtcacgttca ccaagccgcc gcctgccgcc ggcgccgcga
      601 tccggccgcg ctcgatccgg ctctgcaagc tcgccgcgac tttgcccgcc gcttcatcga
      661 gcaactcgtt caacacgtcg gagctgtcct cgctcacgtt cgcggtgccc tgcaccatgc
      721 gcgacgcccg caccgtgtcc gccgtcaaac tcgcgccggt cgtgccatcg agaatcttgt
      781 aactgacgcg caccgtctgc tcgttgttcg tcgtcttcac gccatacgcg ttcaccgagt
      841 tcttcttcga gcccacgctc gaaagcgaaa cctgcagcag ataatccgcg ccgagcgtct
      901 gcgcgagccg caccgccgac gagctttgcg cgagttcctg gtcgagcgcg ttcgctttcg
      961 cgtcggcatc caaaccttgc acggcgttca gcgccgtttc ccgactgatc accgacacgc
     1021 ctttgtccgt cacgccggac gtgatgtagt cctcgagcga cgcgatcttg tcgtcgtgtt
     1081 ccttgcccgc gcggttcgaa acgaaaatcg ccgccgtgta aaccgacggc ttcaccgcca
     1141 cgtccgtgac ccgcgtcgtg atcacgcgct cggtgacggt cttgccgtcc ttctcgtaag
     1201 tgcgcttttc gacggtcgac gacggctgcg cgtcttgcgc gcgcaccaag caggtcgcag
     1261 cggcgatgcc agcgaacaca agacaaaggc ggaggaattt catgggcagg aaaaggacgg
     1321 agccaacgcg cggaacgcct ccactcgctc agcgcacatc gatcgctgca ccgtgcaggc
     1381 gcgcactcgc aacacgcgac gtgagggttt gatacgttga aggcatggac tggacgccgg
     1441 cgggtcgagg ttttcgccaa tacctcggct gtcaagacgg cagcatgcgc gcgctctacg
     1501 catacatgcg tagggcagtc gcttccgcaa ttcgctcgcg ccaggcggcc tcgagttcgc
     1561 acgcttgccc gccgcaccac atcaggcacg ctgccctcac ccatgagcgc cgcctacctc
     1621 ctcgctttgg atcaaggcac cacgtcgtcg cgcgccatcg tcttcgaccg ccgcggccgc
     1681 agtcgcgcca ccgcgcagca ggaatttcct caacacttcc cgcgccccgg ctgggtcgaa
     1741 cacagcccgc gcgatctttg ggaaaccacc cgccgcgtcg cgctcgccgc cgtcggcgaa
     1801 gccaacctca ccgcgcgcaa cctcgcggcc gtcggcctca ccaaccaacg cgaaaccacg
     1861 ttgctctggg accgccgcac cggcgaaccg ctcgcgcccg cgatcgtctg gcaggaccgc
     1921 cgcactgccg cgctttgcgc gcaactca
//
LOCUS       DASPLT010000337         3105 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_250007, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000337 DASPLT010000000
VERSION     DASPLT010000337.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 3105)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3105)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3105
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_250007"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            237..>3105
                     /locus_tag="VEA63_03535"
     CDS             237..>3105
                     /locus_tag="VEA63_03535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HYD83090.1"
                     /translation="MVVPAPTPADFSALAAGFDGELHTGKTLRLLYATDASEYQEIPA
                     AVALPRSERDLRALLAFAQRRRIGLIPRTAGTSLAGQTVGSGIVVDVGRHLHKIVAID
                     AARRRVRVQPGVVRNELNLALRSHGLLFGPETATASRAMIGGMVGNNSCGTNSLVYGS
                     TRDHLVSARGFLSDGSEVTFGPLTSEEFAAKCEGTDSVERRIYREIRDLLGNAENRRL
                     IREHFPKLEVVRRNTGYALDLLMDCAVFDPTSDKPFNLCRLIAGSEGTLFLGVEFELN
                     CEPLPPPPVLLCAHFASVQEALHAMLLALPYRPSAAELIDRPLLECTKQNLEQAKNRF
                     FVDGDPGAILVIELRRESADAAFAELRQMESEMRAAGFGYAFPLVSGDEVARVWDLRR
                     AGQGVMMNVPGDAKPRENVEDTAVAPADLPGYVAELETIMRRYRIDSVYYGHAGAGEL
                     HIRPLFNLRTAEGVKMFRDVATDVAALVKKYRGSLSGEHGDGRLRGEFLRFMVGDACY
                     AMMRRVKAVFDPDGLLNPAKIIDAPPMDTSLRHGPGAPAPHYKTFFDFAPEKGIVEAA
                     EKCSGSGDCRKLHVMGGTMCPSYMATRNEKDTTRARANILRHMLASPRDPANPWNSPE
                     IAEVMELCISCKGCKSECPANVDMARLKAEWQQHYHDARGASWRSRLIANFSRLGALG
                     MVAPRLHNWIVTNAATSSVVKRLAGFALERSLPELPRQTLRRWYAERSDRRAGFSPLR
                     NRSEGGLKPALRGRRVHLFCDEFTNFHDAEIGMKAVRLLERLGYEVVIPEHVDSGRAQ
                     FSKGFLREAQRLAIENVKRLAPVITDDAPLIGLEPSAILGFRDEFPALMPAELVANAK
                     TLARRTFLFEEWIAREAERGHVSREAFTTEARAIKLHGHCHQKALSSLTPAVKALSLP
                     ANYRVEVIPSGCCGMAGSFGYEAEHFAISQQIGELVLFPAVR"
BASE COUNT          537 a          980 c         1023 g          565 t
ORIGIN      
        1 gctgcgcctt ggcatcagca acgattacaa cagccagccg ggcgcaggtg tcgaaaagtt
       61 ggatacgtcg tatttcacgc ggatggtgct gacgttcgac tgatccacgc gtgcagcccg
      121 ctgcgtgagc agcgggattg ccatcgagtc gcgctgcggc cgccaacggt tgcgccgcca
      181 gaatcccccc gagcgtgctc gcctgacggc ggcacgctcg tttcatttcc gacacgatgg
      241 tcgtgcccgc gcctacccct gccgatttct ccgcgctcgc cgccggcttc gatggcgagc
      301 ttcacaccgg gaaaacgctt cggctgctct acgcgaccga cgcgtcggaa taccaggaga
      361 tccccgccgc cgttgcgctg ccccgcagcg aacgcgatct gcgcgcgctg ctcgcgtttg
      421 ctcagcgtcg tcggattggt ctgatcccgc gcaccgcggg cacctccctc gccggccaga
      481 ccgtcggcag cggcatcgtc gtggatgtcg ggcgccactt gcacaagatc gtggcaatcg
      541 atgcggcgcg ccggcgtgtg cgcgtgcagc ccggcgtggt tcgcaacgag ctgaatctcg
      601 cgctgcgatc ccacggcctg ctcttcggcc ccgaaacagc cacggcgtct cgtgcgatga
      661 tcggcggcat ggtgggcaac aactcctgcg ggacaaattc gctcgtttac ggttcgactc
      721 gcgatcacct cgtgtccgcg cggggatttc tcagcgacgg cagtgaggtc acgttcgggc
      781 cgctgacgtc cgaggagttc gccgccaaat gcgagggcac cgattcagtc gaacgccgca
      841 tttaccgcga gattcgcgac ctgctcggca atgcggagaa ccgccgcctg attcgcgagc
      901 atttccccaa actagaagtg gtgcggcgca acaccggtta cgcgctcgac ctgttgatgg
      961 actgcgcggt gttcgacccg acctccgaca aacctttcaa tctgtgccga ctgatcgcgg
     1021 gctccgaagg caccttgttc ctcggtgtcg agttcgagct gaattgcgaa ccgcttccgc
     1081 cgccgccggt gctgctctgc gcgcatttcg cgagcgtcca ggaagcgctg cacgcgatgc
     1141 tgctcgccct accgtatcgc ccaagcgctg ccgagctgat cgaccgcccg ctgctcgaat
     1201 gcacgaaaca aaatctcgag caggcgaaga accggttctt tgtcgacggc gatcccggcg
     1261 cgatcctcgt catcgagctc cggcgcgaat ccgcggacgc agccttcgcc gagttgcggc
     1321 agatggagtc ggagatgcgt gctgcgggtt tcggctatgc gtttcctctc gtgagtggag
     1381 acgaggttgc gcgcgtctgg gatcttcgtc gcgccggaca aggtgtgatg atgaacgtgc
     1441 cgggcgacgc gaagccgcgc gagaacgtcg aagacaccgc ggtcgctccg gcggacctgc
     1501 cgggctacgt cgcggaactc gaaacgatca tgcgccgcta tcgaatcgac agcgtgtatt
     1561 atggacacgc cggcgccggc gaacttcaca tccgcccgct gttcaacttg agaaccgccg
     1621 aaggcgtgaa gatgtttcgc gacgtggcga ccgacgtcgc cgcactcgtt aaaaagtatc
     1681 gcggctcgct cagcggagaa cacggcgatg gccggctgcg cggggaattc ctgcgcttca
     1741 tggtgggcga cgcatgctac gcgatgatgc ggcgcgtgaa agccgtgttc gatccggacg
     1801 gtttgctgaa tccggcgaag atcatcgacg cgccgccgat ggacacctcg ctgcgccacg
     1861 gtcctggcgc gccggcgccg cactataaaa cgtttttcga tttcgcgccg gagaagggca
     1921 tcgtcgaggc ggcggaaaaa tgcagcggct cgggcgattg tcgaaagttg cacgtgatgg
     1981 gcggcacgat gtgcccgagc tacatggcca cgcgcaacga gaaggacacc acgcgagcgc
     2041 gcgccaacat cctccgccac atgctcgcgt caccgcgcga tccggcgaat ccgtggaaca
     2101 gccctgaaat cgcggaggtg atggagctct gcatttcgtg caaaggctgc aaatccgagt
     2161 gtcccgccaa cgtcgacatg gcgcggctca aagccgagtg gcagcagcac tatcacgatg
     2221 cgcgcggcgc gtcatggcgc tcgcgattga tcgcgaattt ctcgcggctc ggggcgctgg
     2281 ggatggtcgc cccgcggttg cacaactgga tcgtcacgaa tgcggcgacg agtagcgtcg
     2341 tgaaacgcct cgccggcttc gccttggagc ggagcttgcc ggagctgccg cgacagacgt
     2401 tgcggcggtg gtatgcggaa cggtcggacc gtagggcagg cttcagcccg cttcggaatc
     2461 gatcggaagg cgggctgaag cccgccctac ggggacgtcg tgtgcatttg ttctgcgacg
     2521 agttcacgaa ttttcacgac gcggagatcg gaatgaaggc ggtgcgcttg cttgagcggc
     2581 tcggctacga ggtcgtgatc ccggagcacg tcgacagcgg tcgcgcgcag ttctcgaaag
     2641 gttttctgcg cgaggcgcag cggctcgcga tcgaaaacgt gaagcggctc gcgcccgtga
     2701 tcaccgacga cgctccgctg atcggtttgg agccgtcggc gattctcggt tttcgcgacg
     2761 agtttcccgc gttgatgccg gcggaacttg tcgccaacgc gaagacgctc gcgcgacgca
     2821 cgttcttgtt cgaagagtgg atcgcgcgcg aagcggagcg cggacacgtc agccgcgagg
     2881 cgttcacgac tgaagcccgc gcgatcaagc tgcacggaca ctgtcatcaa aaagcactgt
     2941 cgtcgctcac gcccgcggtg aaggcgcttt cgctaccggc gaactatcgt gtcgaggtga
     3001 tcccgtcggg ctgctgcggc atggcgggat cgttcggtta cgaggcggag catttcgcga
     3061 tctcgcagca gatcggcgag ctggtgttgt ttcccgcagt gcgcg
//
LOCUS       DASPLT010000338         2544 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_168081, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000338 DASPLT010000000
VERSION     DASPLT010000338.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 2544)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2544)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2544
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_168081"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1003
                     /gene="argH"
                     /locus_tag="VEA63_03540"
     CDS             <1..1003
                     /gene="argH"
                     /locus_tag="VEA63_03540"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007362395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HYD83091.1"
                     /translation="KIRGAERALLAVAQANRDVLIPGYTHLQRAQPVYLAHHLFAWLE
                     MLDRDRARLADVAAHANWCPLGSGAIAGTTLPIDREFTAKALGFVDAKGRPQVTQNSM
                     DTVADRDLFIEFANACAMFGVHASRIAEDLILWSSAEFKFVELPDAFCTGSSLMPQKK
                     NPDSCELLRGKSARLQGNLHTLLTLAKGLPLTYNRDLQEDKPPVFDSFDQTALCADVL
                     AGTLSGMTVYEERCAAAVADPALLATDLADYLVLKGVAFRDAHHAVGAVVRLAETSGV
                     ALDRLPTAEVQKVHPAFGADWVEVFDLKRALAKREGTGMPGPKQVAKQFRRWEKMLG"
     gene            1192..>2544
                     /locus_tag="VEA63_03545"
     CDS             1192..>2544
                     /locus_tag="VEA63_03545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012372964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HYD83092.1"
                     /translation="MKDYASSDLRSFAVVGHASSGKTMLCEAMLACSGRIGRMGSIAA
                     GSTVSDYHASEKQHQISIHASLLNTEWQGKKFNLIDCPGYADFISEALSALRVGDFAL
                     VVINALHGLGIGTDAMWDNATLHGLPRMIAVNALDKSNANFDQVLADARAHFGKNVFP
                     LSIPLDAGPGFSRVLDVLRSEVVTYKPGGNGAYAEAPAEGELAERVHALHKELIEYVA
                     ESDDALMERFFEEGVLAESELRAGLHRAIQNQVFIPLFAVSAETNVGVARLMDFIAKY
                     GSSPLDRSEVDAHDAAGEHVTVPLTHAEPVAYVFKTMNEPGVGELSLFRVYSGTVTSG
                     AELFNASRQVNERLGQVYVLNGHERTPVTQLRAGDLGAVVKLRDTHTDDTLCSPRFPV
                     VLPKVEFPRPNIHGALRLRSKGDEDKLAVGLATLHAEDPTFRYRVDDELHQTIVSGQG
                     E"
BASE COUNT          446 a          642 c          959 g          497 t
ORIGIN      
        1 gaaaattcgc ggcgcggagc gggcgttgct ggcggtcgcg caggcgaatc gcgacgtgct
       61 gattccgggc tacacgcatt tgcagcgggc gcagccggtg tatctggcgc atcatctttt
      121 tgcgtggctt gagatgctcg accgcgatcg ggcgcggctg gcggacgtgg cggcgcatgc
      181 gaattggtgt ccgctcggct cgggtgcgat cgcggggacg acgctgccga tcgatcgcga
      241 gttcacggcg aaggcactgg gttttgtcga cgcgaaaggg cggccgcaag tgacgcagaa
      301 ctcgatggac acggtggcgg atcgcgacct gttcatcgag ttcgcgaatg cgtgcgcgat
      361 gtttggcgtg catgcttcgc ggatcgcgga agatttgatt ttgtggagca gcgccgagtt
      421 caaatttgtg gagctgccgg acgcattttg cacgggatcg tcgttgatgc cgcagaagaa
      481 gaatccggat tcgtgcgagc tgttgcgcgg gaaatcggcg cggttgcagg gaaatttgca
      541 cacgctgctg acgctcgcga agggactgcc gttgacctac aatcgtgatt tgcaggagga
      601 caagccgccg gtgttcgaca gcttcgatca gacggcgttg tgcgcggacg tgctggcggg
      661 aacgctgagc ggcatgacgg tttatgagga gcgttgcgcg gcggcggtgg cggatccggc
      721 gctgctggcg acggatctgg cggactatct tgtgttaaag ggcgtggcgt ttcgggacgc
      781 gcatcatgcg gtcggtgcgg tggtgcggct ggcggaaacg agcggcgtag cgctcgatcg
      841 gttgccgacg gcggaggtgc agaaggtgca tccggcgttt ggcgcggatt gggtggaggt
      901 gttcgatttg aagcgggcgc tcgcgaagcg cgagggcacg gggatgccgg ggccgaagca
      961 ggtcgcgaag cagtttcggc ggtgggagaa gatgttgggg tagggtgggc ggacacggtc
     1021 aggttgaccg tgctacagcg gggtcgggag accccgccct acaatgcgga caaggggtgc
     1081 ggggcgtttt ttagaaaacg cggaggcggc gggacgaaaa tgggttatat tggcggcgga
     1141 atcgttgccg aagcgcggtg gcgatcactg cgcaaccccg gttttttcgt catgaaagac
     1201 tacgcctcgt ccgacctccg cagtttcgcc gtcgtcggac atgcctcgtc cggcaagacc
     1261 atgctctgtg aagcgatgct cgcctgctcg gggcgcatcg gccgcatggg cagcatcgcg
     1321 gccggctcaa cggtgtccga ttatcacgcg agcgagaaac agcatcagat ctcgattcac
     1381 gcgtcgctgc tcaacacgga gtggcaggga aagaagttca acctgatcga ttgtccgggc
     1441 tacgcggatt tcatcagcga ggcgttgagc gcgttgcgcg tcggcgattt cgcgctggtg
     1501 gtgatcaacg cgctgcatgg gctcggcatc gggacggacg cgatgtggga caatgccacg
     1561 ctgcacgggc tgccccggat gatcgcggtc aacgcgctcg ataaatcgaa cgcgaacttc
     1621 gaccaggtgc tcgcggatgc gcgggcgcac ttcgggaaga atgtattccc actttcgata
     1681 ccgctggatg cggggccggg tttcagccgc gtgctcgatg tgctgcgcag cgaagtggtg
     1741 acttacaagc cgggcggcaa cggcgcctac gcggaggcgc cggcggaggg cgagttggcg
     1801 gagcgcgtgc atgcgttgca caaggaattg atcgaatatg tggcggagtc ggacgacgcg
     1861 ttgatggagc ggtttttcga ggaaggtgtg ctggcggaga gtgagctgcg cgcgggattg
     1921 catcgcgcga ttcagaatca ggttttcatt ccgctgttcg cggtgtcggc ggagacgaat
     1981 gtgggcgtgg cgcggctgat ggatttcatc gcaaaatacg gttcgtcgcc gctcgatcgt
     2041 tcggaggtgg acgcgcacga tgcggcgggc gagcacgtga cggtgccgct gacgcacgcg
     2101 gagccggtgg cgtatgtgtt caagacgatg aacgagccgg gagtgggcga gctatcgctg
     2161 ttccgcgttt actccggcac cgtcacaagc ggtgccgaac tttttaatgc gtcacggcag
     2221 gtcaatgaac ggttggggca ggtttatgtt ttgaacgggc atgagcggac gccggtgacg
     2281 cagttgcgcg caggcgatct cggcgcggtg gtaaaactgc gcgacacgca tacggacgac
     2341 acattgtgca gtccgcgttt tccggtggtg ttgccgaagg tggagtttcc gcgtccgaac
     2401 attcatggcg cgctgcggct gcgttcgaaa ggcgacgagg ataaactcgc cgtgggactc
     2461 gcgacactgc acgcggagga tccgacgttt cgttaccgcg tggatgacga gttgcaccag
     2521 acgatcgtgt cggggcaggg ggag
//
LOCUS       DASPLT010000339         1170 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_270240, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000339 DASPLT010000000
VERSION     DASPLT010000339.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1170)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1170)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1170
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_270240"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            92..661
                     /locus_tag="VEA63_03550"
     CDS             92..661
                     /locus_tag="VEA63_03550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HYD83093.1"
                     /translation="MLKRLLLGCALVVSAQAQTSSMQVELANLREDVRGLVQRVGQLS
                     LRVEQLERENAELRGQAGAAQKSFVTLTQLNDAIANLNQIIRSSAASTKAETLQQVGT
                     QMETLAKQTNAAIDSLAKGIATRAPVQTTFSDDYAKEGISYTVQKGDTLALIAKNTGA
                     KLQDIVNANRISDPSRINVGQTLFIPGGK"
     gene            665..>1170
                     /locus_tag="VEA63_03555"
     CDS             665..>1170
                     /locus_tag="VEA63_03555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HYD83094.1"
                     /translation="MSAAPKSRLGRGLGGLIASAVPAKPAPAAAAPAAAPAAAPAPAP
                     AGAPGYLEIAVNLVEPSPYQARREITPEQLSELAESIRSEGLLQPIVVRKVGEKYQLI
                     AGERRWRAFQQLKIKTIPARIVEASNASSAALGLIENLQREGLNPIEEAHGFASLIRD
                     FDLTQETAA"
BASE COUNT          239 a          363 c          367 g          201 t
ORIGIN      
        1 gccgctacgg agaaatgcgg cttggcggcg aagcgtgaat cggcggcaca agccgcttgt
       61 tttcccgcgc gcatttctca agctcacgcg catgttgaaa cgtttgttgt tgggatgtgc
      121 gctcgttgtc agcgcgcagg cccagacctc ttcgatgcag gtggaactcg ccaatctgcg
      181 cgaggacgtt cgcggtctcg tgcagcgcgt gggtcaactt tcccttcgcg tcgaacaact
      241 cgagcgcgag aacgccgagt tgcgcggtca ggcgggcgcc gcgcagaaat cgtttgtcac
      301 gctcacgcag ctcaacgacg cgatagcgaa tctgaaccag atcatccgct cttcggccgc
      361 gagcacgaag gccgagacgc ttcagcaagt cggcacgcag atggaaacgc tcgcgaaaca
      421 aacgaacgcc gcgatcgatt cgctggcgaa aggcatcgcg acgcgcgcac cggtgcagac
      481 aacgttctcc gacgattacg cgaaggaagg catcagttac accgtgcaaa agggcgacac
      541 gctcgcgttg atcgcgaaga acaccggcgc gaaactgcag gatatcgtga acgccaaccg
      601 catctcggat ccctcgcgca tcaacgtcgg ccagacgctc ttcattcccg gaggcaaata
      661 atccatgtcc gcagctccca aatcacgcct cggtcgcggc ctcggcggtc ttatcgccag
      721 tgctgttccc gccaaacccg ctccggccgc tgcggcgccg gctgctgctc cggcggcggc
      781 gcccgcgccc gctccagccg gtgcgcccgg ttatctcgag attgcggtga atctcgtcga
      841 gccgagtccg tatcaggcgc ggcgcgagat cacgcccgag cagttgagcg agctggcgga
      901 gagcattcgc tcggagggat tgctgcaacc gatcgtggtg cggaaggtgg gagaaaaata
      961 tcagttgatc gcgggcgaac gccgctggcg cgcattccaa cagcttaaaa tcaagacgat
     1021 cccggcgcgg attgtggagg cgagcaatgc gtcgtccgca gcgctggggc tcatcgagaa
     1081 tttgcagcgt gaagggctca atccgatcga agaagcacac ggcttcgcga gcctcattcg
     1141 cgatttcgat ctgacgcagg aaacggcggc
//
LOCUS       DASPLT010000340         2622 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_228778, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000340 DASPLT010000000
VERSION     DASPLT010000340.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 2622)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2622)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2622
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_228778"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1785)
                     /locus_tag="VEA63_03560"
     CDS             complement(<1..1785)
                     /locus_tag="VEA63_03560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HYD83095.1"
                     /translation="MSQEPMNNFTPRAQQVLALARKEADRFHHNYVGTEHILLGLIKL
                     GQGVAVSVLQKMGLDLETVRSAVEKQVGTGQETKTQGSIPYTPRVKKVLALAGKEAKT
                     LNHSYVGTEHILLGLLREGEGVAARVLKSLDIDIERTRNEILRELDPQFSSSQSGDVP
                     GEEAAAGTPQRGGGSEDKKEVKTPALKAFGRDLTELARKGEMDPVVGRKNEIRRVVQI
                     LCRRTKNNPVLIGEAGVGKTAIVEGLAQEIASGAVPEILLEKKVITLDLALMVAGTKY
                     RGQFEERIKAVMDEIKRAKNIILFIDELHTIVGAGAAEGAMDASNIFKPALSRGELQC
                     VGATTLNEYRKYIEKDSALDRRFQSVKVEAPSVDDTVLILKGIRSKYEDHHKAIFTDK
                     SIEAAAKLSDRYITGRFLPDKAIDVMDEAGSRARIGALTRPPSVENLTKEIEQVCALK
                     EKAISEQHFEEAARYRDQEKQLRVKQEQITEEWKKAREEKRVTIDEDLMMQVVADWTG
                     IPLNRMEKKESDKLLAMEAELQKAVIGQDMAAVSIARALRRSRADLKDPRRPIGSFMF
                     VGPTGVGKTMLAKQLAMQMFGNQEAIIQI"
     gene            complement(1799..>2622)
                     /locus_tag="VEA63_03565"
     CDS             complement(1799..>2622)
                     /locus_tag="VEA63_03565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP--guanido phosphotransferase"
                     /protein_id="HYD83096.1"
                     /translation="ISRELSGAKSGSGVVISKDQCFSVMINEEDHLRIQVLRAGFQLK
                     KAWASINELDTEIEDRIDYAFSPSLGYLTACPTNLGTGMRASAMMHLPALVIANQMEK
                     VVRAVNQLGMVVRGLFGEGSDASGSIFQISNQTTLGESEDEIIKRLGSVLTSIVEHEL
                     NARQKLLEADPGKLFDKIGRAYGILQNSHVLSSGEAMNLLSLLRLGIDLRVFPEANRS
                     CIDRLFIEAQPGHLQHAQKGEFEPGQRDLLRAGRLRAEFANFPQPNFTFTTDGSN"
BASE COUNT          456 a          834 c          764 g          568 t
ORIGIN      
        1 gatctggatg atcgcctctt ggttgccgaa catctgcatc gcgagttgct tcgcgagcat
       61 cgttttgccg acgcccgtcg ggccgacgaa catgaacgaa ccgatcggac ggcgcggatc
      121 cttgaggtcc gcacgggaac gacggagcgc acgtgcgatc gagaccgccg ccatgtcctg
      181 gccgatgacg gctttctgca gctcggcctc catcgccagc agcttgtcgc tctctttctt
      241 ctccatccgg ttgagcggaa tgccggtcca gtcggcgacg acctgcatca tgaggtcctc
      301 gtcgatggtc acgcgctttt cctcgcgcgc cttcttccac tcttcggtga tctgttcctg
      361 cttcacgcgc agctgcttct cctggtcgcg ataacgcgcg gcttcttcga agtgctgctc
      421 gctaatggct ttttccttca gcgcgcaaac ctgctcgatc tccttcgtga ggttctcgac
      481 ggacggcgga cgcgtcagcg caccgatgcg cgcgcgcgaa cccgcttcgt ccatgacgtc
      541 gatcgccttg tcgggcagaa aacgtcccgt gatgtagcgg tcggacaact tcgccgccgc
      601 ttcgatcgac ttgtcggtga agatcgcctt gtggtgatcc tcatacttcg agcgaattcc
      661 cttcagaatc agcacggtgt cgtcgaccga cggcgcttcg accttcacgg attggaaacg
      721 gcgatcgagc gcagagtcct tctcaatata cttgcggtat tcgttaaggg tcgtcgcacc
      781 gacgcactgc agttcaccac gcgagagcgc aggcttgaag atgtttgacg cgtccatcgc
      841 gccttcggcg gcgccggcgc cgacgatcgt gtgcagctcg tcgatgaaca ggatgatgtt
      901 cttcgcgcgc ttgatctcgt ccatcaccgc cttgatgcgc tcctcgaact ggccgcggta
      961 tttcgtgcct gcaaccatga gggcgaggtc gagcgtgatg accttctttt ccaagaggat
     1021 ctccggcacg gcgccgctgg cgatttcctg cgcgaggcct tcgacgatcg cggtcttgcc
     1081 gacacccgct tcgccgatga gcacgggatt gttcttcgtg cgacggcaga ggatctggac
     1141 gacgcggcgg atttcgtttt tccggccgac aaccggatcc atttcgcctt tgcgcgcgag
     1201 ttcggtgagg tcgcggccaa acgctttcag cgcgggcgtc ttcacctctt tcttgtcttc
     1261 cgagccgccg ccgcgctgcg gcgtgccggc cgcggcttct tcgccaggga cgtcaccgct
     1321 ctggctgctg gagaactgcg gatcgagttc acggaggatt tcgttgcgcg tgcgctcgat
     1381 gtcgatgtcg agcgacttca acacgcgcgc cgccacgccc tcgccctcgc ggagcaagcc
     1441 aagcagaatg tgctcggtgc cgacatagga atggttgagc gtcttcgctt ccttgccagc
     1501 gagggcgagg acttttttga cacgaggcgt gtaaggaatg ctgccctggg tcttcgtctc
     1561 ctgaccggtg ccgacctgct tttcaacggc cgagcgcacg gtctcgaggt ccagacccat
     1621 tttctgcaac acgctcacag cgacgccctg gccgagcttg atcagcccga gcaggatgtg
     1681 ctctgtcccc acataattgt ggtggaaccg gtcggcctcc ttgcgggcca acgcgagcac
     1741 ctgctgcgcg cgcggcgtga aattgttcat gggttcttgg gacatgtgta ttaaaggctt
     1801 aattcgaccc gtcggtcgtg aaagtaaagt tgggttgcgg gaaattggca aactccgccc
     1861 ggagtcgccc cgcccgcaaa aggtcgcgct gcccaggctc gaactcgccc ttctgcgcgt
     1921 gttgaagatg ccccggctgc gcctcgatga aaagccggtc gatgcacgaa cggtttgcct
     1981 cgggaaacac ccggagatcg atgcccagcc gcaacagcga gaggagattc atcgcctcgc
     2041 cagaactgag cacgtgactg ttttgaagta tgccgtaagc gcgaccgatc ttgtcgaaca
     2101 acttgccggg gtcggcctcg agcagcttct ggcgcgcgtt caactcgtgc tcgacgatcg
     2161 acgtcagcac gctgccgaga cgcttgatga tctcgtcctc agattcgccg agcgtcgttt
     2221 gattcgaaat ctggaaaata ctgccgctcg cgtccgatcc ttcgccaaac aaaccgcgca
     2281 cgaccatgcc gagctggttc accgcgcgca caaccttctc catctggttc gcaatcacga
     2341 gcgcgggcag gtgcatcatc gccgacgcac gcatgccggt cccgagattg gtcggacaag
     2401 ccgtgagata accgagcgat ggcgagaacg cgtagtcgat ccgatcctcg atctccgtat
     2461 cgagctcgtt gatcgaggcc cacgctttct tcaactgaaa gcccgcgcgc aaaacctgga
     2521 tgcgcagatg atcctcctcg ttgatcatca ccgaaaagca ctggtccttg ctgatcacca
     2581 cgcccgagcc gctcttcgcg ccgctcaact cacgactgat ca
//
LOCUS       DASPLT010000341         1409 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_86517, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000341 DASPLT010000000
VERSION     DASPLT010000341.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1409)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1409)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1409
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_86517"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1409)
                     /locus_tag="VEA63_03570"
     CDS             complement(<1..>1409)
                     /locus_tag="VEA63_03570"
                     /inference="COORDINATES: protein motif:HMM:NF012343.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HYD83097.1"
                     /translation="ILLALTRWQAVHLLPVDWFYRILTFHGLNMLIFWILFFEVAVLY
                     FACTVPLKARLFSRKVAWVSFGMMLVGAVMVDWIILKGQADVLMTSYLPLLAHPAFYL
                     GIILVAVGTLVGVFNFFATLYTAKRDRTYEGSMPLVTFGATAAAIIAVVTLLHGAIVM
                     IPTFAYSMDWIGQPDPMWYRMIWWGLGHQSQQVNVCAMVAIWYLIAHLSTGAKPVNET
                     VCRGAFVLYILFINLASAHHLLVDPGVGSAWKIWNTSYAMYLAVLASMIHGFTVPASV
                     EIAMREKGYVKGLFGWVTSAPWRNPAFAGFVLSLVIFGFIGGITGVTLGTQQINIIAH
                     NTLRITGHFHATVVGGTTLAFMAVTFYVIPLIFRRDFIWRGAARLQPWLFGGGILLVS
                     IGMSFAGSMGVPRRDYDIEPAGIFGPAAHLFLGVMGIGAILAVIGLFLFVVLCVGTIL
                     FGEKNAGRPIADWGTAKAM"
BASE COUNT          357 a          510 c          344 g          198 t
ORIGIN      
        1 ccatcgcctt cgccgtcccc cagtccgcga tcggccggcc cgcgttcttc tcaccgaaca
       61 aaatcgtgcc gacgcacaac accacaaaca ggaacaaacc gatcaccgcg agaatcgcgc
      121 caatccccat tacgccaaga aacagatgcg ccgccggacc aaagatcccc gccggctcga
      181 tatcgtaatc ccgccgcggc acgcccatcg accccgcgaa cgacatgccg atcgacacca
      241 gcaaaattcc gccgccaaac aaccacggct gcaaccgcgc cgcgccacgc cagatgaagt
      301 cgcgccggaa aatcagcggg atcacataaa acgtcaccgc catgaacgcc agcgtcgtgc
      361 cacccacgac cgtcgcgtgg aaatgccccg tgatgcgcag cgtgttgtgc gcgatgatat
      421 tgatctgctg cgtgccgagc gtcacgcccg tgatcccgcc gatgaatccg aaaatcacca
      481 gtgacaacac gaaccccgcg aacgccggat tccgccacgg cgccgacgtc acccagccga
      541 acaggccttt gacataacct ttctcccgca tcgcgatctc gaccgacgcc ggcaccgtga
      601 acccgtggat catcgacgcc agcaccgcga gatacatcgc gtaagacgtg ttccagatct
      661 tccacgccga acccacgccc gggtcgacca gcaaatggtg cgccgacgcc agattgatga
      721 acaaaatgta gagcacgaat gcgccgcgac acaccgtttc gttgaccggc ttcgcccccg
      781 tcgacagatg cgcgatcaaa taccagatcg ccaccatcgc gcacacgttg acctgctgcg
      841 actggtgccc gagtccccac cagatcatcc gataccacat cggatccggc tgcccgatcc
      901 agtccatcga ataggcaaac gtcgggatca tcacgatcgc gccatgcaac aacgtcacca
      961 ccgcaatgat cgccgccgct gtcgcaccaa acgtcaccaa cggcatcgag ccttcgtaag
     1021 tccgatcgcg cttcgccgtg tagagcgtcg cgaaaaaatt gaacaccccc acgagcgtgc
     1081 cgaccgcgac cagaatgatc cccagataaa acgccggatg cgccagcagc ggcagataac
     1141 tcgtcatcaa aacatccgcc tgacccttga gaatgatcca gtcgaccatc accgcgccga
     1201 caagcatcat gccgaacgac acccacgcga ccttccgcga aaacaaccgc gctttcaacg
     1261 gcaccgtgca cgcgaaatag agcactgcga cttcgaaaaa caggatccag aaaatgagca
     1321 tgttcagccc gtggaacgtc aggatccggt agaaccagtc gaccggcagc aggtgaaccg
     1381 cctgccagcg cgtgagcgcc agcaggatt
//
LOCUS       DASPLT010000342         2032 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_168199, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000342 DASPLT010000000
VERSION     DASPLT010000342.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 2032)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2032)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2032
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_168199"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(123..1232)
                     /gene="hisC"
                     /locus_tag="VEA63_03575"
     CDS             complement(123..1232)
                     /gene="hisC"
                     /locus_tag="VEA63_03575"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HYD83098.1"
                     /translation="MSGLSSLALPHVAKLHAYTPGLQPSEAGWVKLNTNECPYPPSPR
                     VAEAVRRELGEDGANLRLYPNPKSTPLRAAIAALHGLREENVIVGNGSDDILNLLIRV
                     FVTSNAAAGFTFPSYSLYPVLVEIQDGRSTIIEFDRSMKLPIERIANSDARAFFLTSP
                     NAPTGVGFSNAEIEQVLARFRGLLIVDEAYAPFADENAVALLARYPRLVVVRTLSKAY
                     ALAGIRVGYALAHPEAIDLLDRVRDSYNVNRLSQAAALAAIGDTAHLAGLVSRVKTTR
                     DAHLARFVGERGWFTYPSQANFIFTEPKNARGESGPAVAKSAYDFLFSRKVLVRYFAS
                     HALTASFLRISVGTDDEMLVLQHTLDAWPQATPNV"
     gene            complement(1355..>2032)
                     /locus_tag="VEA63_03580"
     CDS             complement(1355..>2032)
                     /locus_tag="VEA63_03580"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HYD83099.1"
                     /translation="KIFGPGNAYTCEAMRQVFGIVGVNGLPGPSEVMIIADETARADY
                     AAADLLAQAEHGSGREKIYLVATSSAIIAAISAQIDEQLESLSRSEKMRRVLDEGFLA
                     VEVKTLAQAADVANYVAPEHLELLVDDAAAKKLTKAITTAGAIMIGNDTPTALGDFTA
                     GPSHVLPTGRAGRFFSGMRVADFLRRTSLIRYDRKSVKKGEPIVAAFAAMERLDAHGR
                     SVKIRTR"
BASE COUNT          395 a          638 c          637 g          362 t
ORIGIN      
        1 cgcaaacagc gtcagcatgt gatcgaagaa cggcacgccg gtctcgatct tcgccacgcc
       61 gcggccatcg accgcgagcg tgagcgcgat gtcggtttcc gccgtctggc ggtttacggt
      121 cgctacacgt tgggagttgc ctgaggccat gcgtcgaggg tgtgttggag gacgagcatc
      181 tcgtcgtccg tgccgacgct gatgcggagg aaggaagcgg tcaaggcgtg gctcgcgaag
      241 tagcggacga gcaccttccg cgagaaaagg aagtcgtaag ccgacttcgc caccgccggc
      301 ccgctctcgc cgcgcgcatt tttcggttcg gtgaaaatga aattcgcctg cgacggatac
      361 gtgaaccaac cgcgttcgcc gacgaaacga gcgaggtgcg cgtcacgcgt tgtcttcacc
      421 cgactgacga ggccggcaag gtgggccgtg tcgccgatcg cggcgagcgc ggcggcctgc
      481 gagaggcggt tgacgttgta gctatcgcgc acgcgatcga gcaggtcgat tgcttcggga
      541 tgcgccaggg cgtaaccaac gcgaatcccc gcgagcgcat acgctttcga aagcgtgcga
      601 accacgacga gacgcggata acgcgcgagc agtgcgacgg cgttttcgtc cgcgaacggc
      661 gcataggctt cgtcgacgat caacaagccg cgaaagcgcg cgagcacctg ctcgatctcg
      721 gcattgctga agcccacgcc cgtcggcgcg ttcggcgacg tgaggaagaa cgcacgcgca
      781 tccgaattcg cgatacgctc gatcggcagc ttcatcgagc ggtcgaattc gatgatcgtg
      841 ctgcgtccgt cctgaatttc gacgagcacc ggataaagcg agtagctcgg aaacgtaaag
      901 ccggcagcgg cgttcgatgt gacgaacacc cgaatcagta ggttaaggat gtcgtccgag
      961 ccgttgccga cgattacgtt ttcctcgcgc agaccgtgca gcgccgcgat cgccgcacgc
     1021 aacggcgtgc ttttcggatt cggataaagc cgcaagttgg cgccgtcttc gcccaactcc
     1081 cggcgcaccg cctcggcgac ccgcggactc ggcggatagg ggcactcgtt ggtgttgagt
     1141 ttcacccacc ccgcttcgct cggctggagg ccgggcgtat aagcgtgaag cttcgcaacg
     1201 tgcggaagag cgagtgacga caaaccggac atccgtcgat ggccgcaaaa acgcgcaaaa
     1261 ggcgcaaaga gataattact ccgatcgtgg ctcgctggtg agcagcggga tggaacgcaa
     1321 tttccccgcg ctcacgcgca gagccacgaa gaggctatct cgtccggatc ttcaccgacc
     1381 ggccgtgcgc gtccaaccgc tccatcgcgg cgaatgcagc gacgatcggt tcgccttttt
     1441 tcacgctctt ccggtcgtag cgaatcaagc tcgtccggcg cagaaaatcc gccacgcgca
     1501 tcccgctgaa aaaccggccg gcgcgtcccg tcgggagaac gtggctcgga cccgccgtaa
     1561 aatcgccgag cgcggtcggc gtatcgttgc cgatcataat cgcgcccgcc gtcgtgatcg
     1621 ccttcgtgag cttcttcgca gccgcatcat cgactagcag ttcaagatgc tcgggtgcga
     1681 cgtagttcgc gacgtccgca gcctgcgcca gcgttttcac ttcaactgcg agaaaacctt
     1741 cgtcgagcac gcggcgcatt ttctccgagc gcgaaagcga ctcgagctgc tcgtcgattt
     1801 gcgccgaaat cgcagcgatg atcgccgacg aggtcgcgac gagatagatt ttttcgcgac
     1861 ccgaaccgtg ctcggcctgc gcgagcaggt ccgccgcagc gtaatcggcc cgcgccgttt
     1921 cgtcggcgat gatcatcacc tcgctcgggc cgggaagtcc gttgacaccc acgatgccga
     1981 aaacctgtcg catcgcttcg caggtatacg cgttacccgg tccgaaaatc tt
//
LOCUS       DASPLT010000343         1056 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_188631, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000343 DASPLT010000000
VERSION     DASPLT010000343.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1056)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1056)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1056
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_188631"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(219..>1056)
                     /locus_tag="VEA63_03585"
     CDS             complement(219..>1056)
                     /locus_tag="VEA63_03585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HYD83100.1"
                     /translation="FAISNHLVGQAICDPIDERHKSILSPAIWGDGNPEGVRKRAAKQ
                     LALTAKTARAFFDAKPGAEKGAAAVVNGFTGSSIWHSIYAFPPTSQAYWDAGYADFAK
                     RFGPILETFDKLNVNFGLEVHPTEIAFDIASAQRALAAVKHHKRFGFNYDPSHLGYQG
                     VDYVKFIRTFSDRIFHAHMKDVWWGHGNGDVGVFGGHTTFGDARRQWDFRSVGRGDIN
                     FEEIIVALNDVGYAGPLSVEWEDIRMDRVHGASESAAFLRKLDFARSTVAFDAAFDKK
                     NQ"
BASE COUNT          193 a          270 c          392 g          201 t
ORIGIN      
        1 ggaggagaag gcgccggcga cgagctcggc ctggccgtcc atttgagagg cgatgcgatg
       61 aacgccgccg atgaaggcgc cgcgtccgcc gccgatcatg ccgtaacgaa gtttgcgggt
      121 gagagccatg aggagaattt gttttacagg agggaacgga ggaaacggag aggagcggcg
      181 ggtgcggctc tgttttctcc gttagcttct gtgaatgctt actggttctt tttgtcgaag
      241 gcggcgtcga aggcgacggt gctgcgagcg aagtcgagct tgcgaaggaa ggcggcggat
      301 tcgctcgcgc cgtgaacgcg gtccatacgg atgtcttccc attcgacgga gagcgggccg
      361 gcgtagccga cgtcgtttaa cgcgacgatg atttcctcga agttaatgtc gccgcggccg
      421 acggagcgga aatcccactg gcggcgggcg tcgccgaaag tcgtgtggcc gccgaagacg
      481 ccgacgtcgc catttccgtg gccccaccag acgtccttca tgtgcgcgtg gaagatgcga
      541 tcggagaacg tgcggatgaa cttcacgtag tcgacgccct gatagccgag gtgagagggg
      601 tcatagttga aaccgaagcg cttgtggtgc ttcacggcgg cgagggcgcg ctgcgcgctg
      661 gcgatgtcga acgcgatctc ggtcgggtga acttcgagac cgaagttgac gttgagtttg
      721 tcgaacgtct cgaggatcgg gccgaagcgc ttggcgaagt cggcgtaacc agcatcccaa
      781 tacgcctgcg aggtcggggg aaaggcgtag atcgagtgcc agatggagga gccggtgaag
      841 ccgttgacga ccgcggcggc gcctttttcc gcgccgggtt tggcatcgaa gaacgcgcgc
      901 gcggttttcg cggtgagggc gagttgtttg gcggcgcgct tgcggacgcc ttcgggattg
      961 ccgtcgcccc agatggccgg cgaaaggatg gatttatggc gttcgtcgat cgggtcgcag
     1021 atcgcctggc cgacgaggtg gttggaaatt gcgaag
//
LOCUS       DASPLT010000344         2429 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_209405, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000344 DASPLT010000000
VERSION     DASPLT010000344.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 2429)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2429)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2429
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_209405"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1344)
                     /locus_tag="VEA63_03590"
     CDS             complement(<1..1344)
                     /locus_tag="VEA63_03590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HYD83101.1"
                     /translation="MDQKPGGDAGLHLKRETTGLAGIDRVLHGIEPGDNIVWEVDTIE
                     EYEELVGPYVAAAKAAGRRLIYFRFAEHPPLVDTNDGVEVCPVDTSAGFEKVVRQIHE
                     VIERAGTRVLYVFDCLSHLSDRWVSDQSLGNFFMLTCPRLRDLDTVTYFAIYRDKHSL
                     YALEPIRRTTQFLLDVFRLDLRLYVRPVKVQHRSREAMNTIHVRQGGEFLPVKDSAVL
                     AQILSRTQWPRLQSDGRAGYWRRLFQEAAQVAKDWREERCPPERRAEMTKKVFAAFRV
                     HRSGIASLVERHLTLEDFLLVRDRMIGIGSVGGKTLGMLVARAILRDRDPDLASRLEA
                     HDSFFVGAEVFLTFLVQSGVWWLREQQRNPATFLEGLDEGRRRILEGVFPEHIAEQFR
                     GMLDYFGESPYIVRSSSILEDARGNAFSGKYESVFVVNRGSREERMTTLLAAVRKV"
     gene            complement(1433..>2429)
                     /gene="gdhA"
                     /locus_tag="VEA63_03595"
     CDS             complement(1433..>2429)
                     /gene="gdhA"
                     /locus_tag="VEA63_03595"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HYD83102.1"
                     /translation="ALTGLPMGGGKGGSNFNPKGKSANEVMRFCQSFMTELYRHIGSD
                     TDVPAGDIGVGGREVGYLFGQYKRLANEFTGVFTGKGIKWGGSLIRPEATGYGAVYFA
                     EEMLKTRGESLAGKVCTVSGSGNVAQFTVEKLLQLGAKPVTLSDSDGSIYDPAGFTTE
                     KLAWVMQLKNARRGRIREYTEKFGGEFLAGKRPWSVPCHCAFPSATQNEIDGEDAKTL
                     LKNGCTLVSEGANMPSTPDAVDAYLSAGILYGPGKAANAGGVATSGLEMSQNSMRLSW
                     SREEVDQRLHRIMVNIHENCRATAADYGAPGNYVLGANIAGFTKVADAMLEQGVV"
BASE COUNT          447 a          887 c          647 g          448 t
ORIGIN      
        1 gaccttccgc accgccgcaa gcagcgtggt cattcgctct tcacgcgatc cgcgattcac
       61 gacgaacacg ctttcgtatt ttcccgaaaa cgcatttccg cgcgcgtcct ccaggatcga
      121 actcgagcgc acgatatacg gcgactctcc gaagtaatcc agcatcccgc gaaactgctc
      181 ggcgatgtgc tcgggaaaca caccctcgag aatccgccgc cgtccttcat ccaatccttc
      241 gagaaacgtc gccggatttc gttgctgctc ccgcagccac cacacgccgc tctgcaccag
      301 aaacgtgagg aacacttccg cgcccacgaa aaaagaatcg tgcgcctcga gccggctcgc
      361 tagatccgga tcgcggtcgc gcaaaatcgc gcgcgccacc agcatcccga gcgtttttcc
      421 gccgaccgat ccgatcccga tcatccgatc gcgcacgagt agaaaatcct cgagcgtcag
      481 atgccgttcc acgagcgatg cgattcccga tcgatgcacg cgaaacgccg cgaacacctt
      541 cttcgtcatc tccgctcgcc gctccggcgg acaccgttcc tcgcgccaat ccttcgccac
      601 ctgcgccgcc tcctgaaaca gccgccgcca atagcccgcc cgcccgtcgc tttgcagccg
      661 tggccactgc gtgcgcgaca gaatctgcgc aagcaccgcg ctgtccttca ccggcaaaaa
      721 ctcgcctcct tgccgcacgt gaatcgtgtt catcgcctcc cgcgaacgat gctgcacttt
      781 caccggccgc acatacagcc ggaggtcgag gcggaaaaca tccagcagga actgcgttgt
      841 ccgccggatc ggttcgagcg cgtagagcga atgcttgtcc cgatagatcg cgaagtaggt
      901 gaccgtatcc agatcgcgga gacgcgggca cgtcagcatg aaaaagttgc ccaggctctg
      961 gtcggaaacc caccggtcgg acaggtgtga aaggcagtcg aacacgtaaa gcacccgcgt
     1021 gccggcgcgc tcgatcacct cgtgaatctg tcgcaccacc ttttcgaagc ctgcgctcgt
     1081 atcgacgggg cagacttcca cgccgtcatt cgtgtcgacg agcggcggat gctccgcgaa
     1141 ccggaaataa atcaaccgcc gcccggccgc cttcgccgcc gccacatacg gcccgaccag
     1201 ttcctcatac tcttcgatcg tgtcgacctc ccacacgatg ttgtcgcccg gctcgatccc
     1261 gtgcagcacg cgatcgatcc ccgcaagccc ggtcgtctcg cgtttgaggt ggagcccggc
     1321 gtccccgccg ggcttctgat ccatcgcagc cgtatcagcc cgacgaggac gtcgggctcc
     1381 acccccgttc tcttcgtcct cagctcgcga gctcatcggc cgccggttgc gctcacacga
     1441 cgccctgctc gagcatcgcg tcggcgacct tcgtgaaacc tgcgatgttc gcccccagca
     1501 cgtaattgcc cggagcaccg tagtccgccg cggttgcgcg acagttctcg tgaatgttca
     1561 ccatgattcg gtgcagccgt tggtcgactt cctcgcgcga ccaactcagc cgcatcgaat
     1621 tctgcgacat ctccaagccc gacgtcgcca caccgcccgc attcgcggct ttgccgggtc
     1681 catagagaat gcctgcggac aaatacgcgt ccaccgcatc gggtgtcgac ggcatgttcg
     1741 cgccttcgga caccagcgtg cagccattct tcaacaacgt cttcgcatct tcgccgtcga
     1801 tctcgttctg cgtcgcgctc gggaacgcgc aatgacacgg cacggaccac ggccgctttc
     1861 cggccaggaa ttcgccgcca aatttttccg tgtattcgcg aatccgcccc cggcgcgcgt
     1921 tcttcagttg catcacccac gcgagcttct ccgtcgtgaa accggccgga tcgtaaatgc
     1981 tgccgtcgga gtccgacaac gtcaccggct tggcgccgag ctgcagcagc ttctcgaccg
     2041 taaattgcgc gacattgccg gagcccgaca cggtgcagac tttccccgcg agtgactcgc
     2101 cgcgggtctt caacatctcc tccgcgaaat acaccgcgcc ataaccggtt gcctccgggc
     2161 ggatcagcga gccaccccac ttgatgcctt tgccggtgaa gacgcccgtg aactcgttgg
     2221 ccagccgctt gtattgaccg aacaaatacc cgacctcgcg cccgccgacg ccgatgtcgc
     2281 ccgccggcac gtccgtgtcc gacccgatgt gccggtagag ctccgtcatg aagctctggc
     2341 agaagcgcat cacctcattg gcgctcttac ccttcgggtt gaagttcgag ccgcccttgc
     2401 cgccgcccat cggcaggccg gtgagcgcg
//
LOCUS       DASPLT010000345         1123 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_228851, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000345 DASPLT010000000
VERSION     DASPLT010000345.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1123)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1123)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1123
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_228851"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1123
                     /locus_tag="VEA63_03600"
     CDS             <1..>1123
                     /locus_tag="VEA63_03600"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HYD83103.1"
                     /translation="DGYEVCRRLRADEDLRHVPIILLTALDDRQSKLKGLEAGADDFL
                     SKPCDSVELRTRLRTITRLNRFRQLFEERARFETAVAYAPDAMVLADDSGRILLRNAA
                     FRQLIAPDFANAEALSIFDYFAPDDSRVLVEQLTQLREVGRRLGPFETELQHARHRDT
                     RVEVTVGRLPGHGSVLAFNIRDITEKTQLQTQLLRAQRIELLGQLAGGIVHDVNNILT
                     AISGNAMLIEDSEPERVPLYIENIQKSVRRGASLLRQILMFARGGDQELSPTSTALLV
                     HETSAILNEVLGKSIKVTLSAPDGLPDILGDNNQLHQVLMNFCVNARDAMPGGGTLHI
                     ATARAQLSAADAQRLSPLARAGDFVTVSVRDSGSGMPPEV"
BASE COUNT          208 a          406 c          307 g          202 t
ORIGIN      
        1 cgatggctac gaagtctgcc gccgcctccg cgccgacgag gacctgcgcc acgtgccgat
       61 catcctgcta accgcactcg acgaccgaca gtcgaagctg aagggcctcg aagcgggtgc
      121 ggacgatttt ctcagcaagc cctgcgattc ggtcgagctt cgcacccggc ttcgcacgat
      181 cacgcgtctc aatcgttttc gccagctctt cgaagaacgc gcgcgtttcg agaccgccgt
      241 cgcctacgcg ccagacgcga tggttctcgc tgacgattcc ggccgaatcc tcctgcgcaa
      301 tgccgcgttt cgccagctca tcgctcccga tttcgcgaat gccgaggcgc tctcgatctt
      361 cgactacttc gcccccgacg actctcgggt tctcgtcgag caactgaccc aactccgcga
      421 agtgggccgc cggctcgggc cgttcgaaac ggagcttcaa cacgcgcgtc atcgcgatac
      481 tcgcgttgaa gtgaccgtcg gtcgtctccc cggccatggc tccgtgctcg cgttcaacat
      541 tcgcgacatc accgaaaaga cccagcttca gacgcaactc ctgcgtgcgc agcgcatcga
      601 gcttctgggg caacttgccg gcggcatcgt gcacgacgtg aacaatattc tcaccgcgat
      661 cagcggcaat gcgatgctca tcgaagattc cgagcccgag cgtgtgccgc tctacatcga
      721 gaacatccag aaaagcgtcc gccgcggcgc cagcctcctc cgtcaaatcc tgatgttcgc
      781 ccgcggcgga gatcaggaac tcagcccgac gagcaccgcg ctgctcgtcc acgagacgtc
      841 cgccatcctc aacgaagttc tcggcaaaag catcaaagtc accctctcgg cgccggacgg
      901 attgcccgat attctcggcg acaacaatca gctgcaccaa gtgctgatga acttctgcgt
      961 caatgcccgc gatgccatgc ccggcggtgg caccttgcac atcgccaccg cgcgcgccca
     1021 gctctccgcc gccgatgcgc aaaggctaag ccccctcgcg cgcgccggcg attttgtgac
     1081 ggtcagcgtt cgcgattccg gcagcggcat gccgcccgaa gtc
//
LOCUS       DASPLT010000346         1959 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_168306, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000346 DASPLT010000000
VERSION     DASPLT010000346.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1959)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1959)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1959
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_168306"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..278
                     /locus_tag="VEA63_03605"
     CDS             <1..278
                     /locus_tag="VEA63_03605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HYD83104.1"
                     /translation="ASYSLENGLTFSANAVVTGEMNNNTAGTLVIPTQFTIDASVSYK
                     FDKWSLRAQVLNVTDEENWSPPNSVYGNGSILALPGTQLQVTAKYSF"
     gene            567..1439
                     /locus_tag="VEA63_03610"
     CDS             567..1439
                     /locus_tag="VEA63_03610"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HYD83105.1"
                     /translation="MRVIDSHVHLYPAEVDIDPAGWAAAREEKHWALLCTRRRRDGRP
                     VQSLPTLDGLLQAMDAGGIARAVLLGWYWEKHDTCAWQNRFYAECVRAHPDRLSAFAT
                     FHPAAGRAVVIEELRRAQGEGLIGIGELSPHSQGFRVDDPVFGEALTLAAELKMPVNV
                     HASDPESRRFPGWVETPAEDFVWLARLFPRTNFVLAHWGGMLAVRDATLREFANVYYD
                     TAASPLMYDAGVWSRVVPVLGAERVLFGSDFPLNLYPRVSVAAEMARFAAEAREAGMV
                     GDVMGGNAARLLGI"
BASE COUNT          320 a          473 c          778 g          388 t
ORIGIN      
        1 tggcgtctta ctcgctggag aacggcctga cgttctcggc caacgcggtc gtgacgggtg
       61 agatgaacaa caacacggcg ggcacgctcg tgatcccgac gcaattcacg atcgacgcgt
      121 cggtgtccta caagttcgac aaatggagcc tgcgggcgca ggtgctcaat gtcacggatg
      181 aggaaaactg gtcaccgccg aattcggttt acggcaacgg ctcgatcctc gcgctgccgg
      241 ggacgcagct gcaggtgacg gcgaaataca gtttctgatt ctggcggatg ctggttttgc
      301 cgtgggcgag gctggtggct cggcggctgg cgttggacgt agcgcggtca acctgaccgc
      361 ggatggaagg caggcacggt cagattgacc gtgctacctt cggcggggga gttgcgcggc
      421 gcggcggttg cgcggtggcg gggtcgggag accccgccct agacgggatg tgggctcgtg
      481 cagggtgaat ccaaggcggg ctgaagcccg ccctacttgg gtttcggttg cgttaggggc
      541 gggggcggag cgaggcttgg agacacatgc gtgtgatcga tagtcatgtt catctttatc
      601 cggcggaggt ggatatcgac ccggcgggtt gggcggcggc gagagaggag aaacattggg
      661 cgctgctttg cacccggcgg cggcgcgacg ggcggccggt gcaatcgctg cccacgctcg
      721 atggtttgtt gcaggcgatg gacgcgggcg gaatcgcgcg cgcggtgttg ctcggctggt
      781 attgggaaaa gcatgacacg tgcgcgtggc agaatcggtt ttacgcggag tgcgtgcgcg
      841 cgcacccgga ccggctgtcg gcgttcgcga catttcatcc ggcggcgggg cgtgcggttg
      901 tgatcgagga gttgcgacgc gcgcagggcg aagggctgat tggaatcgga gaactgtcgc
      961 cgcactcgca ggggtttcgc gtggacgatc cggttttcgg cgaggcgctc acgctggcgg
     1021 cggaattgaa gatgccggtg aacgtgcacg cgtcggaccc ggagagccgg cgatttccgg
     1081 ggtgggtgga gacgccggcg gaggatttcg tgtggctggc gcggctgttt ccgcgaacga
     1141 actttgtgct cgcgcactgg ggcgggatgc tggcggtgcg cgatgcgacg ctgcgggagt
     1201 ttgcgaatgt ttactacgac acggcggcct cgccgttgat gtatgatgcc ggcgtgtggt
     1261 cgcgggtggt gccggtgttg ggcgcggagc gcgtgttgtt tgggtcggat tttccgctga
     1321 acctttatcc acgggtgtcg gtggcggcgg agatggcgcg gttcgcggct gaagcgcgcg
     1381 aggcggggat ggtgggcgac gtgatgggtg ggaatgctgc gcggctgttg gggatatgag
     1441 gcgccgtgat gaacgtccta gccgtcttca tgcagtagac ctcccgcgaa tcacgcgaat
     1501 gaacgcgaat ttcagaacga tgaaatcaaa tcgggccgct tcaggggccg ggtcgctgat
     1561 gagtgaacga tcacttctcg tcgtttgtat tcggatcaat tagcgtgatt cgcgggcaac
     1621 tgaattgttc cggtttagag agaaaccacg gatggacacg gatgaggagt gggctggcgg
     1681 cgagatggcg gtgtgggaaa tttttttttg aaccacgaat ggacacgaat gaacacgaat
     1741 agagtgcccg tgggtcgttg ggcgattctg gtttgacggg tgtttatcgc ggctcggttg
     1801 aatgagtttt gcggaggcac cacgcgacgg cggggctgcg cgggtgcggg acgatgattt
     1861 gcaggccggt ggtgaggccg gacggcagcg cgacggggag cgtgacgacg ggcaggccgc
     1921 cgatgctggc gggcgcggtg agcgtgagga ggcgcgtgc
//
LOCUS       DASPLT010000347         1211 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_270412, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000347 DASPLT010000000
VERSION     DASPLT010000347.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1211)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1211)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1211
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_270412"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..187
                     /locus_tag="VEA63_03615"
     CDS             <1..187
                     /locus_tag="VEA63_03615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HYD83106.1"
                     /translation="SLRAKDPAYRLDRIAAQFGGGGHACAAGLNARESTTEFRKRLVA
                     ALAQQLAAVDAQKEAHS"
     gene            184..912
                     /gene="truB"
                     /locus_tag="VEA63_03620"
     CDS             184..912
                     /gene="truB"
                     /locus_tag="VEA63_03620"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HYD83107.1"
                     /translation="MIFQQRNELEGVLLVDKPGDHTSHDVVARLRRKLNMKRIGHAGT
                     LDPMATGLMILLIGKATKISQYLISLDKEYEGAIELGKVTDSQDADGEVLETRPVPPL
                     TEAEVRTAMAGFLGDQYQIPPMYSAIKIDGQPLYKSARKGVEVEREPRFIRVMSFELT
                     KFGMPKFEFRLRCTKGTYVRTVAHDLGQRLGCGAHLAALRRTATDKFNVTQALTLDQI
                     EALSLKEIEQRLIPVHQAAPSIAL"
     gene            909..>1211
                     /locus_tag="VEA63_03625"
     CDS             909..>1211
                     /locus_tag="VEA63_03625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HYD83108.1"
                     /translation="MNEIVQFGALEAAVLPSRPLHLAIGIFDGLHLGHRAVIDAAVQS
                     ARRSGGQAGVLTFEPHPSVLLRPQSPTRLLMSRSAKARVLARLGVDAMITQPFTHEF"
BASE COUNT          233 a          341 c          422 g          215 t
ORIGIN      
        1 aagtctgcgc gcgaaggacc cggcctatcg gctcgatcga attgcggcgc aatttggcgg
       61 cggcgggcat gcatgcgcgg cgggtttgaa cgcgcgggag agcacgacgg aatttcgaaa
      121 gcgactggtg gcggcgctgg cgcaacaact cgcggcggtc gacgcgcaga aagaggccca
      181 ctcatgattt ttcagcagcg taacgaactc gaaggtgtgt tgctcgtgga caagccgggc
      241 gatcacacgt cgcacgacgt cgtggcgcgg ctgcgccgca agttgaacat gaaacgcatc
      301 ggccacgccg gcacgctcga tccgatggcg accgggctga tgattctgtt gatcggcaag
      361 gccacgaaaa tttcgcagta tttgatcagc ctggacaagg aatacgaagg cgcgatcgag
      421 ctcggcaagg tgacggattc gcaggacgcc gatggcgagg tgttggaaac gcggccggtg
      481 ccgccgctga cggaagcgga agtgcgcacc gcgatggcgg gtttccttgg cgaccaatac
      541 caaattccgc cgatgtattc ggcgatcaag atcgacggcc agccgctcta caaaagcgca
      601 cgcaaaggcg tggaggtgga gcgcgagccg cgctttattc gggtgatgag ttttgagctg
      661 acgaagttcg ggatgccgaa attcgagttt cgtttgcgct gcacgaaggg cacttatgtg
      721 cgaacggtcg cgcacgatct cggccagagg ctcggttgtg gggcgcacct cgcggcgctc
      781 cgtcgcacgg caaccgacaa gtttaacgtc acgcaggcgt tgaccctcga ccagatcgag
      841 gcgctctcgc tgaaagaaat cgaacagcgg ctgattcccg ttcaccaagc cgcgcccagc
      901 atcgcgttgt gaacgagatc gttcagttcg gtgcgctcga ggcggcggtg cttccgtcgc
      961 gaccgctgca tctggcgatc ggaattttcg acggactgca tctgggccat cgcgcggtga
     1021 tcgacgctgc ggtgcaatca gcgcggcgca gcggcgggca ggcgggcgtg ctgacgttcg
     1081 agccacatcc gagcgtgctg ctgcggccgc agtcgccgac gcgtttgttg atgagccggt
     1141 cggcgaaggc gcgcgtgctg gcgaggctgg gcgtggacgc gatgatcacg cagccgttca
     1201 cgcatgagtt t
//
LOCUS       DASPLT010000348         2333 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_65408, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000348 DASPLT010000000
VERSION     DASPLT010000348.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 2333)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2333)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2333
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_65408"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..795
                     /locus_tag="VEA63_03630"
     CDS             <1..795
                     /locus_tag="VEA63_03630"
                     /inference="COORDINATES: protein motif:HMM:NF012742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HYD83109.1"
                     /translation="RLIESNHLPLFLWHNHQTVSEEYARQQIHRLLSRWVDGVILWPF
                     FANIYAKHLHEFQTRNIPLVIIDHTIPGTEADVVESDESQIAAQIVAHLRTLNHRHFL
                     IVSGPLHIGWADNRAQSIRNELEKHAGVTAQELRVPQPSSIPSESKELAEVSRMIAER
                     LHQQPQITAVIACTDIIAQSVYDAAHALAWSIPNRLSVVGVADLNFAEVMSPALTTVR
                     QNGYTIGRQAAQVELERSAGLLTGPSRLFQVETTLVSRQSTAVASH"
     gene            complement(811..1269)
                     /locus_tag="VEA63_03635"
     CDS             complement(811..1269)
                     /locus_tag="VEA63_03635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SufE family protein"
                     /protein_id="HYD83110.1"
                     /translation="MSTYPPNLAEIVDLFEHLPDEEKRENLIAFADQAAGCAPKAGEA
                     FDLEDTRKDEECTDTVGVFLRVDPATRAMHYRINLGPHVQTLTRAMTAILCKGLDGAT
                     PEAVLDVPQDFVPKIVGGQLVRLRSQTVYYILTRMKSAAKVWLNRERAIA"
     gene            complement(1381..2256)
                     /locus_tag="VEA63_03640"
     CDS             complement(1381..2256)
                     /locus_tag="VEA63_03640"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HYD83111.1"
                     /translation="MNPKFSAYANPDVLVDGAWLQEHLNDPTIRIVESNEDVLLYDTG
                     HIPGAVHIDWRGDLQDNTQRDYVSPERFAEVCSTHGITPDTTVVFYGDKSNWWAAYAL
                     WAFRLFGHTKVKLLDGGSTKWKADGRPITRDKPKHAPTKYPVPAKRHDDEIRAFYSDT
                     LAHAQARKPLIDVRSPGEYKGEITHMPEYPQEGVLRGGHVPGARSVPWKIAANDDGTF
                     KTFDELKKIYTENLGLKKGDDIIAYCRIGERSSHTWFVLTYLLGYDKVRNYDGSWTEW
                     GNMVRSPIAKGEQPG"
BASE COUNT          448 a          823 c          620 g          442 t
ORIGIN      
        1 cgtctcatcg agtccaacca cctgccgctc ttcctgtggc acaaccacca gaccgtatcc
       61 gaagaatacg cacgccagca gattcaccgc ctgctcagcc gctgggtcga cggcgtgatt
      121 ctctggcctt tcttcgccaa catctacgcg aagcacctgc acgagtttca aacgcgcaac
      181 atcccgctcg tcatcatcga tcacaccata cccgggacgg aggccgacgt cgtcgaaagc
      241 gacgaaagcc agatcgccgc gcagattgtg gctcatcttc gcacgctgaa ccaccgccac
      301 ttcctgatcg tttccgggcc gctgcacatc ggctgggccg acaaccgcgc gcagtccatc
      361 cgtaacgagc tcgaaaaaca cgccggcgtc accgcccagg aactgcgcgt gccccagcct
      421 tcgagcatcc cttcggagtc gaaagagctc gccgaagtca gccgcatgat cgcggagcgc
      481 ctccatcagc agccgcaaat caccgccgtg atcgcctgca ccgacatcat cgcacaaagc
      541 gtctacgacg ccgcgcacgc cctggcctgg tcgatcccca accggctgag cgtcgtcggc
      601 gtcgccgacc tcaacttcgc ggaagtgatg tcgccggcgc tcaccaccgt gcgacaaaac
      661 ggctacacca ttggccgcca ggccgctcaa gtggagctcg agcgcagcgc cggcctgctc
      721 acgggccctt cacgcctctt ccaggtcgaa accaccctgg tgtcacgaca atcgaccgcc
      781 gtcgcatcgc actgagcgcc acgcgccgcc tcacgctatc gcccgctcgc ggttgagcca
      841 caccttcgcc gcgctcttca ttcgcgtcag gatgtaatac accgtctggc tccgcagccg
      901 cacgagttgc ccgcccacga tcttcggcac gaaatcctgc ggaacgtcca acaccgcctc
      961 gggcgtcgcg ccgtccagcc ctttgcacaa gatcgccgtc atcgcgcgcg tgagcgtctg
     1021 cacgtgcggc ccgagattga tccgataatg catcgcgcgc gtcgcaggat cgacgcgcag
     1081 gaaaacgccc accgtgtccg tgcactcctc gtccttgcgc gtgtcttcga gatcgaatgc
     1141 ctcgcccgcc ttcggtgcgc agcccgccgc ttgatcggca aacgcgatca gattctcgcg
     1201 tttctcctcg tcaggcaggt gctcgaacaa atcgacgatc tcagccaaat tcggcgggta
     1261 ggtgctcatc tcattttcaa ttctcgattt ttgattttcg acttcaaacc cgccgtgtgt
     1321 cgcgaaccgc gaaccacaaa acgggaatcg aaaatcgaaa ttccaaaatc aaaaatctgc
     1381 tcagcccggc tgctcgccct tcgcgatcgg cgaacgcacc atgttgcccc actcggtcca
     1441 cgagccgtcg taattgcgca ccttgtcata cccgagcaga tacgtgagca cgaaccaggt
     1501 gtgactcgag cgctcgccga tccggcaata cgcgatgatg tcgtcgccct tcttcaatcc
     1561 gaggttctcg gtgtaaatct tcttcaactc gtcgaacgtt ttgaacgtgc cgtcgtcatt
     1621 cgccgcgatt ttccacggca cactccgcgc gcccggcacg tgaccgccgc gcagcacgcc
     1681 ttcctgcgga tactccggca tgtgcgtgat ctcgcccttg tattcgcccg gcgaacgcac
     1741 gtcgatcagc ggcttgcgcg cctgcgcatg agcgagcgta tccgaataaa aagcgcggat
     1801 ctcgtcgtcg tgacgtttcg ccggcaccgg atacttcgtg ggagcatgct tcggcttatc
     1861 gcgcgtgatc ggccggccat cggccttcca cttcgtgctg ccgccgtcga gcagcttcac
     1921 cttcgtatgg ccaaacagcc gaaacgccca caacgcatac gccgcccacc agttcgattt
     1981 gtcgccgtaa aaaacgaccg tggtgtcggg cgtgatgccg tgggtcgagc atacctcggc
     2041 gaaacgctcc ggcgacacgt agtcgcgctg cgtgttgtcc tgcaggtcgc cgcgccagtc
     2101 gatgtgcacg gcgccgggga tgtggccggt gtcgtaaagc agcacatctt cgttgctctc
     2161 gacgatgcgg atcgtgggat cgttcagatg ttcctgcaac caggcgccat cgaccaacac
     2221 gtctggattc gcgtaggcgg aaaacttcgg attcatgagg gctcaacctg ccgcctcgcc
     2281 gcagcaaagg caaaccgcgc gccgaatttc atctccgcca gtccgtcata aac
//
LOCUS       DASPLT010000349         1444 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_147781, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000349 DASPLT010000000
VERSION     DASPLT010000349.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1444)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1444)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1444
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_147781"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..227
                     /locus_tag="VEA63_03645"
     CDS             <1..227
                     /locus_tag="VEA63_03645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008767 - UDP-galactopyranose mutase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
                     /protein_id="HYD83112.1"
                     /translation="EFPRSEGDPYYPIPRADNAALYKRYHALAVETPEVHFVGRLGTY
                     RYYNMDQVVGQALTLYNKLANTTRAAALAV"
     gene            371..>1444
                     /locus_tag="VEA63_03650"
     CDS             371..>1444
                     /locus_tag="VEA63_03650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2"
                     /protein_id="HYD83113.1"
                     /translation="MFPSNAIPRPEYPRPDRQRGLVEGVDWLNLNGPWQFRFDPQRRG
                     HDEQWFNPAGPDWREQIIVPFCWESLAAWGEGDAAGNDNYYATRVYVKPLEVTRENHR
                     IAERHEVGWYRRHIEIPDSHHWRNRRVILTVGAADFATDCWSNGTHCGRREGGYVPLE
                     FDITDTLGPSDGKVRRAIIVLRVEDPMDNHEQPVGKQWGWYTPTSGIWQTVFVEPRPQ
                     VYIDHFRIKTDIDNGRVSFELVCPGITENITAVVDIIPPEAPPHRVTLDPPVNSVASG
                     DAEIYPVSLWDPDAPKLYRIILRLIDEEREIDTVRSYFGMRKIGTVPAASDDRPAMLS
                     LNNKPIYLRGALYQSYHPDGVYTA"
BASE COUNT          269 a          539 c          384 g          252 t
ORIGIN      
        1 acgaattccc gcgctccgag ggcgatccgt attatccgat tccgcgcgcc gacaacgccg
       61 cgctctacaa gcgctaccac gcgctcgccg tcgagacgcc cgaagtccat ttcgtcggcc
      121 ggctcggcac gtatcgctac tacaacatgg accaggtcgt cggccaggcc ctcacgctct
      181 acaacaagct cgccaacacc acgcgcgccg ccgccctcgc ggtgtgaacc gtttactttc
      241 tccgtagtct agtagggcgg gcttcagccc gccattctcc gcacgaccgc tctccggccg
      301 tgctttctcc ttttcgccga taacgcctcg cgcgttcgca acttcatttc cgcgccacgc
      361 cacttccgac atgtttccct ccaacgccat cccgcgcccc gaatatccac gccccgatcg
      421 gcaacgcggc ctcgtcgaag gcgtggactg gttgaacctc aatggaccct ggcaattccg
      481 tttcgatccg cagcgccgcg gccacgacga gcaatggttc aaccccgccg gccccgactg
      541 gcgcgaacaa atcatcgtcc cgttctgctg ggaatcgctt gccgcctggg gcgaaggcga
      601 tgccgcgggc aacgacaact actacgccac gcgcgtttac gtaaaaccgc tcgaggtcac
      661 ccgcgaaaat caccgcatcg ccgagcgtca cgaagtcggc tggtatcgcc ggcatatcga
      721 aattccggat tcgcaccact ggcggaaccg ccgcgtcatc ctcaccgtcg gcgccgccga
      781 tttcgccacc gactgctgga gcaacggcac gcactgcggc cgccgcgagg gcggctacgt
      841 tccgctcgag ttcgacatca ccgacacgct cgggccgtcc gacggcaaag tccgccgcgc
      901 gatcatcgtc ctccgcgtcg aggatccgat ggacaaccac gaacagcccg tcggcaagca
      961 gtggggctgg tatacgccca ccagcgggat ctggcagacg gtcttcgtcg agccgagacc
     1021 gcaggtctac atcgatcact tccgcatcaa aacggacatc gataacggcc gcgtttcctt
     1081 cgaactcgtc tgtcccggta tcaccgaaaa catcacagcc gtcgtcgaca tcatcccgcc
     1141 ggaggcgccg ccgcaccggg tcacgctcga ccctccggtg aactccgtcg cctcgggcga
     1201 tgcggaaatt tatccggtgt cgctgtggga tcccgacgcg ccaaaactct accggatcat
     1261 tcttcgcctc atcgacgagg agcgcgagat cgacacggtt cgtagctatt tcggcatgcg
     1321 caaaatcggc accgtgcccg ccgcatccga cgaccgcccc gcgatgctct cgctgaacaa
     1381 caagccgatc tatctgcgcg gcgcgctcta ccagtcgtat catcccgacg gcgtctacac
     1441 cgcc
//
LOCUS       DASPLT010000350         1028 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_228989, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000350 DASPLT010000000
VERSION     DASPLT010000350.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1028)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1028)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1028
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_228989"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..995)
                     /locus_tag="VEA63_03655"
     CDS             complement(<1..995)
                     /locus_tag="VEA63_03655"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HYD83114.1"
                     /translation="MTYFEAMVKLHERFLEEKADVALPPLEGGIVFIGQTVTGKADVG
                     PTPRSAISPLVLIHANLVHNVLTDDLAKRAPEWAVWLGAVLLGYAGVAIGLKRSVWAL
                     AAFGLASAGVYVIAAYAVWIEFSVWLPMMAPLLGFAALHFLIIGRRVREEQKGREQVK
                     QMFGTYLSPELLKRMMRDGRNIAAVSSERRPVTILFCDLRDFTSMAEQLKDDALIAQL
                     NEYLAAMVECIHAEAGTLHKFIGDAVMAVWGDLASEGIERDARRAARAALAMQAVLGR
                     LNEAWVRDGKPAFRMGVGLNHGVVLIGNVGSPRRMEFTAIGDAVNLASRLESLNK"
BASE COUNT          194 a          411 c          247 g          176 t
ORIGIN      
        1 tccttgttca ggctctcgag tcgcgatgcg aggttcaccg cgtcgccgat cgcggtgaat
       61 tccattcgcc gcggcgatcc cacattcccg atcaacacga caccgtggtt caatcccacg
      121 cccatccgaa acgccggctt cccgtcgcgc acccacgcct cgttcagccg tccgagcacc
      181 gcctgcatcg ccagcgccgc gcgcgcggct cgccgcgcat ctctctcgat cccttcgctc
      241 gcgagatcgc cccacaccgc catcaccgcg tcgccgatga atttgtgcag cgtgccagcc
      301 tccgcgtgaa tgcactccac catcgccgcg agatattcgt tcaactgcgc gatcaatgcg
      361 tcatctttca actgctccgc catgctcgtg aaatcccgca gatcgcaaaa caggatcgtc
      421 accggccgcc gctcgctact caccgccgca atgttgcgcc catcgcgcat catccgcttc
      481 agcaactccg gcgacagata cgtgccgaac atctgcttca cctgctcgcg ccccttttgc
      541 tcctctcgca cgcgccggcc gatgatcaaa aaatgcagcg ccgcaaaacc gagcagcggc
      601 gccatcatcg gcagccacac gctgaactcg atccacaccg catacgccgc gatcacatac
      661 acccccgcgc tcgccaggcc aaacgcggcg agcgcccaca ccgagcgttt caacccgatc
      721 gccacgcccg cataacccag cagcaccgca cccagccaca ccgcccactc cggcgcgcgt
      781 ttggccagat cgtccgtcag cacgttatgc acgagattcg catggatcag caccagcggc
      841 gaaatcgcgc tccgcggcgt cggtccgacg tccgccttgc ccgtcaccgt ttgcccgatg
      901 aacacgatgc cgccctcgag cggcggcagc gccacgtcgg ctttctcctc caagaagcgt
      961 tcgtgcaact tcaccatcgc ttcgaaatag gtcatcagca cgaagtccga tccgtgctcg
     1021 tccagatc
//
LOCUS       DASPLT010000351         2544 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_4690, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000351 DASPLT010000000
VERSION     DASPLT010000351.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 2544)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2544)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2544
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4690"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(5..517)
                     /locus_tag="VEA63_03660"
     CDS             complement(5..517)
                     /locus_tag="VEA63_03660"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HYD83115.1"
                     /translation="MGLPGGARSRAAFTLLEILIAVALLGLLSAALVTGANHLLDNRA
                     RTPAEVFWEAARDARRTALESATEVRLSFSEKEKTFVADDGRGQKTFSLVEPPRELTV
                     SFLPAQSTNGSLLIGGQLVETEAVPFVSFYPDGTCTPFRIQFRTTGPATVIAIDPWTC
                     APMLLDEENR"
     gene            complement(522..980)
                     /gene="gspG"
                     /locus_tag="VEA63_03665"
     CDS             complement(522..980)
                     /gene="gspG"
                     /locus_tag="VEA63_03665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009508054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system major pseudopilin GspG"
                     /protein_id="HYD83116.1"
                     /translation="MPRSFPHPRSRNALRRAFTLLEIMIALAILGLLVGLGVSNLDKI
                     FGGAQSTTAQLFVNESIKLPLTSYRIHMGDYPSTAEGLQALIAAPGNRAGQWHGPYLQ
                     DGRVPLDPWGEPYGYRYPGTKNKTGYDAFSKGPDRTEGTADDIGNWSEEK"
     gene            complement(1064..1139)
                     /locus_tag="VEA63_03670"
     tRNA            complement(1064..1139)
                     /locus_tag="VEA63_03670"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1103..1105),aa:Arg,seq:cct)
     gene            1244..1441
                     /locus_tag="VEA63_03675"
     CDS             1244..1441
                     /locus_tag="VEA63_03675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007360183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bS18 family ribosomal protein"
                     /protein_id="HYD83117.1"
                     /translation="MSTSEQPTQQSLTPDQIPFTTPQAMRGYVTDTGKILPRKYTHLS
                     AKQQRAITKSIKQSRNMLLAL"
     gene            1501..2490
                     /locus_tag="VEA63_03680"
     CDS             1501..2490
                     /locus_tag="VEA63_03680"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HYD83118.1"
                     /translation="MKVARTARIYRGTPRNLARLARALRAGELVAVPTETVYGLAANA
                     LDARACAKIFHAKGRPTNDPLIVHIHALDQLSLIAEPNEAAFKLARAFWPGPLTLVLP
                     KRQVVPNIVSSGLPSVAVRMPAHRLFRRLLKLAGLPLAAPSANPFGYVSPTSAAHVRA
                     NLRDKISYILDGGASTIGVESTIVDLREPARPRVLRPGAVTRDDIERVLGVTVRAKKP
                     ASRGAQVAPGLLARHYSPRTPAVLHRALSPAAARASRSEAWIFITRPEGARGSNIFWL
                     DERGDLRGVARRLFATLRRVDSGGFTKIHLERAEGGGLADAINDRLARAAARD"
BASE COUNT          484 a          799 c          807 g          454 t
ORIGIN      
        1 catttcagcg gttctcctcg tccaacaaca tcggcgcaca cgtccacgga tcgatcgcga
       61 tcacggttgc ggggcctgtc gtgcgaaact gaatacggaa cggcgtgcac gtgccatccg
      121 gatagaacga gacgaacggc actgcttccg tctcgacgag ttgtccgccg atgagcaagg
      181 agccgttggt cgattgcgcc ggcagaaaac tcacggtgag ttcgcgcggc ggctccacga
      241 gagaaaacgt tttttggccg cgaccatcgt cggcgacaaa tgtcttttct ttctcgctga
      301 aactgagccg cacctcggtc gccgactcca gcgcggtgcg gcgtgcgtcg cgggcggcct
      361 cccaaaaaac ttccgccggc gtgcgggcgc ggttgtcgag gagatggttg gcccccgtga
      421 cgagggccgc gctgagcagt cccagcagcg cgacggcgat gagaatttcg agcagcgtga
      481 atgctgcgcg cgaacgtgca ccgcccggca aacccattcg gttacttctc ctccgaccag
      541 ttgccgatgt cgtccgcggt gccttcggtg cggtccggac ccttggaaaa cgcatcataa
      601 ccggtcttat tcttggttcc gggataacga taaccatagg gctcgcccca aggatcgagc
      661 ggcacccgac cgtcttgcaa ataaggcccg tgccactggc cggcgcgatt acccggtgcg
      721 gcgatcaacg cctgcaatcc ttccgccgtg ctcgggtagt cgcccatgtg gatgcgatag
      781 ctggtgagcg gaagcttgat cgactcgttc acaaaaagct gggcggtcgt ggactgagct
      841 ccgccaaaaa ttttatcgag attgctgacg ccgagtccga cgagcaaacc gaggatcgcg
      901 agggcgatca tgatctcgag cagcgtgaaa gcgcggcgca aggcgttgcg ggagcgaggg
      961 tgaggaaatg agcggggcat gacaggcaac agactggcct tagagggacg aatgtcgaga
     1021 cgcgcgctgc ccgcgattgt ttccgaaaaa tacccgccaa tgctggcgcc cccacccgga
     1081 cttgaaccgg gatgcgcggt ttaggaaacc gctgctctat ccagttgagc tatggggacg
     1141 gacaacgcga cgaacatgga cgcatcgcga ggcggtttgc atctcaaaaa tgcaggttga
     1201 catcacccgg agcgaagact gtgttgacca ccctttttcc gacatgagca cttccgaaca
     1261 gccgacgcag cagagcctca cccccgatca aattccgttc accacgccgc aggcgatgcg
     1321 gggctacgtg acggacacgg gcaagatcct gccgcgcaaa tacacgcacc tttccgctaa
     1381 acaacagcgc gcgatcacga agagcatcaa gcagtcccgc aacatgctgc tcgcgctgta
     1441 agcgccgaaa tcgttaaacc tttcgggccg gggtcgaaga ctccggcctt tttcgtgtcc
     1501 atgaaagtcg cccgcaccgc tcgtatttat cgtggcaccc cgcggaacct cgcccggctg
     1561 gcgcgagctt tgagagcggg cgaactcgtc gcggtgccca ccgaaacggt ttacggcttg
     1621 gcggccaacg cgctcgacgc gcgcgcctgt gcgaagattt ttcacgcgaa aggtcggccg
     1681 acgaacgatc cattgatcgt tcacatccac gcgctcgatc agctgtcgtt gatcgcagag
     1741 cccaacgaag ccgccttcaa actcgcgcgt gcgttctggc cgggtccgct cacgctcgtg
     1801 ctaccgaagc gccaagtcgt cccgaatatc gtttcgtcgg gcctgccgag cgtcgcggtg
     1861 cgcatgccgg cgcaccggtt gtttcgacga ttgttgaaac tggcgggact gcctctggca
     1921 gcgcccagcg caaatccgtt cggctacgtc agccccacct cggcggcgca tgtccgcgcc
     1981 aatttgcgcg ataaaatttc ctacattctc gatggcggcg cgtcgacgat cggagtcgag
     2041 tcgacgatcg tcgatctgcg ggagccagcg aggccgcgcg tgctgcggcc gggcgcggtg
     2101 acacgcgacg atatcgaacg tgtgctcggc gtcaccgtgc gcgcgaagaa gccggcgtcg
     2161 cgcggcgcac aagtcgcgcc gggtttgctg gcgcgacact acagcccgcg cacgcccgcg
     2221 gtgttgcacc gcgcgttgtc accagcggcg gcgcgtgctt cgcgaagcga agcgtggatt
     2281 ttcatcacgc ggccagaggg cgcgcgcgga tcgaatattt tttggctgga tgaacgcggc
     2341 gatctccgcg gcgtcgcgcg gcgtctgttc gccacgctgc ggcgggtgga ttccggcggt
     2401 ttcaccaaga tccatcttga acgcgccgaa ggcggcgggc tggcagacgc gatcaacgat
     2461 cggctcgccc gggctgcggc gcgcgactag acgagcagat ggctcgcgcg cagcaggacg
     2521 ccgcgctcag cgcacgttgg cttc
//
LOCUS       DASPLT010000352         1426 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_44205, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000352 DASPLT010000000
VERSION     DASPLT010000352.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1426)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1426)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1426
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_44205"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..244
                     /locus_tag="VEA63_03685"
     CDS             <1..244
                     /locus_tag="VEA63_03685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD83119.1"
                     /translation="RGVESEIWVYARTIEGKPRPMTTGTRDVPYVDPLTGAARTIKEP
                     VYGYEQTTITEETELLMLDGALVQWKQQAFSERDYR"
     gene            295..>1426
                     /locus_tag="VEA63_03690"
     CDS             295..>1426
                     /locus_tag="VEA63_03690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012377114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046559 - alpha-glucuronidase activity
                     [Evidence IEA];
                     GO_process: GO:0045493 - xylan catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucuronidase family glycosyl hydrolase"
                     /protein_id="HYD83120.1"
                     /translation="MRHIRLPSRLSGVRHGLLFLVFAALATFASADDGYRLWLRYDEI
                     SDKSLRSTYAEAITEIVVPETPAFPPWAGGTVNAARDELVAGLSGLLGVDVPVVKQPT
                     RAGALVLATPRDPAIAALISEADLRTASEEGYILRRVEMNGQPRTLIVANRNIGLLYG
                     AFAFLRHLQTQQPLDHLNLVSAPKIQRRLLNHWDNLNGTVERGGAGNSLWDWFQLPDF
                     VSPRYRDYARACASIGINGTVLTNVNANALVLTEPYLLKVAAIANVLRPYGIRVYLTA
                     RFSAPTEIGGLKTADPLDPTVQEWWRAKTDEIYRHIPDFGGFLVKANSEGQPGPQAYG
                     RNHADGANMLADALQPRNGIVMWRAFVYDNNVPDDRHKQAYDE"
BASE COUNT          273 a          561 c          349 g          243 t
ORIGIN      
        1 ccggggcgtc gaaagcgaga tctgggtcta cgcgcgcacg atcgagggca aaccccgacc
       61 catgacgacc ggcacgcgcg acgtgcccta cgtcgatcct ctcaccggcg ccgcccgcac
      121 gatcaaagaa cctgtttacg gctacgagca aaccaccatc accgaagaaa cggagctcct
      181 gatgctcgac ggcgcgcttg tgcaatggaa acaacaggcc ttttccgaac gcgactaccg
      241 ctgaccagca cctgatcctc gttcttcgct tctctcagcc cactcccaaa acctatgcga
      301 cacattcgcc ttccttcccg cctttccgga gttcgccacg gattgctctt cctcgttttc
      361 gccgccctcg ccacgttcgc gtccgccgac gacggctacc gtctctggct ccgctacgac
      421 gagatcagcg acaagtcgct ccgctccacc tacgccgaag cgatcactga aatcgtcgtc
      481 cccgaaacac ccgcctttcc accgtgggcc ggcggcaccg tcaacgccgc gcgcgacgaa
      541 ctcgtcgccg gcctgtccgg tctcctcggc gtcgacgttc ccgtcgttaa acaacccacc
      601 cgcgcgggcg cacttgttct cgccacgccg cgcgacccgg cgatcgccgc gctcatcagc
      661 gaagccgatc tccgcaccgc gagcgaagaa ggctacatcc tccggcgcgt cgagatgaac
      721 ggccagcccc gcacgctcat cgtcgccaac cgcaacatcg gcctcctcta cggcgccttc
      781 gcgttcctcc gtcatctgca aacccagcaa ccactcgacc atctcaacct cgttagcgcg
      841 ccaaaaatcc agcgccgcct tcttaatcac tgggacaatc tcaacggcac cgtcgagcgc
      901 ggcggcgccg gcaactcgct ctgggattgg tttcaactcc ccgacttcgt ttcgccgcgc
      961 taccgcgact acgcccgtgc ctgcgcgtcc atcggcatca acggcaccgt cctcaccaac
     1021 gtcaacgcca acgccctcgt tctcaccgag ccctacctgc tcaaggtcgc cgccatcgcc
     1081 aacgttcttc gcccttacgg tatccgcgtt taccttacag cccgcttcag cgcgcccacg
     1141 gaaatcggtg gcttgaaaac cgccgatccg ctcgatccca ccgtgcagga atggtggcgc
     1201 gcaaaaaccg acgagattta ccgccacatt cccgacttcg gcggcttcct cgtgaaggcg
     1261 aactccgaag gccagccggg tccgcaagcc tacggccgca atcacgccga cggcgccaac
     1321 atgctcgccg acgcgcttca gcctcgcaac ggcatcgtga tgtggcgcgc cttcgtctac
     1381 gacaacaacg tccccgacga tcgccacaaa caagcctacg acgagt
//
LOCUS       DASPLT010000353         2342 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_209517, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000353 DASPLT010000000
VERSION     DASPLT010000353.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 2342)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2342)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2342
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_209517"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1188)
                     /locus_tag="VEA63_03695"
     CDS             complement(<1..1188)
                     /locus_tag="VEA63_03695"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HYD83121.1"
                     /translation="MNAPLTSPFTAPLRAVLALGLLVFASRSLAQTVAPAAEHEADDP
                     AVLSVFEVHTARDTGYAAENTTSGSRLNTKLADTPSPISVMTADFIRDIGATTIEDLA
                     AYSINTEPLFGMAGDVANGNEHSTGRAELRARGLPTTRTVNFFACEGEVDTYNTERVE
                     FARGANALLFGIGKPGGVFNTTTKKPNLRTSDASFTLRAGDWSQLRASADLNLALLRD
                     KLGVRINALDESRESWRPNEGRDAERFAVATRWQINRRLRFDVEFETNRVETQTQRKW
                     PSFDSYTTWHEAGENIDAGGNNAVINAQRTALGIQTQAGLNTTYWVWNDTDNQLVNYS
                     ATNAANIQTKSAIVRAPVTPDNPTPGNTPQENPLLLDFDVVPKLVTIGGPGIGNITDQ
                     DVLT"
     gene            1305..>2342
                     /locus_tag="VEA63_03700"
     CDS             1305..>2342
                     /locus_tag="VEA63_03700"
                     /inference="COORDINATES: protein motif:HMM:NF012742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HYD83122.1"
                     /translation="MPSTAPVKLQVIADQVGVSRTTVSLALRNHAKIPGATRERIKRV
                     AEELGYRPNPLVSAHMALLRTLQPKHTGLCLAFVCNRSLREVMADSLTPLRLYYEGAK
                     ARAEETGYALQYFNLRERGMTERRFSQILLARGIHGVVIAPLSEGSGVSNVELQWESF
                     ALAMIEHTFLEPRLHKVCNDEFSTVGRLIQRLLDYGHERIGIAMHSRMDDHANHLWLA
                     GFQAFQALTDKRHRVPHFITTAWNKTAFLAWYRKHRPEAIITINSDIVGWLREAGYDV
                     PEEVSCASLYWRKERNNVSGYYQSHELMAAAAVDHVIGQLNRNERGLPASEKTTLIQA
                     EWREGATLRRKT"
BASE COUNT          406 a          604 c          880 g          452 t
ORIGIN      
        1 ggtcaacaca tcctggtcgg tgatgttgcc gatgccgggt ccgccgatgg tgacgagttt
       61 cgggacgacg tcgaaatcga ggaggagagg attttcctgg ggcgtgttgc cgggagtcgg
      121 attgtcggga gtgacgggag cgcgaacgat ggcggacttc gtttggatgt tagcggcgtt
      181 ggtggcgctg tagttgacga gttgattgtc ggtgtcgttc cagacccagt aagtcgtatt
      241 gagtccggcc tgagtttgaa tgccgagggc ggtgcgctgg gcgttgatga cggcgttatt
      301 gccgccggcg tcgatgtttt cgcccgcttc gtgccacgtg gtgtaggaat cgaagctggg
      361 ccatttgcgc tgagtctggg tttcgacgcg gttggtttcg aattcgacgt cgaagcgcag
      421 acgacgattg atctgccagc gcgtggcaac ggcgaagcgt tcggcgtcgc gaccttcgtt
      481 aggccgccag gattcgcgcg attcatcgag ggcgttgatg cgaacgccga gtttgtcgcg
      541 gaggagcgcg aggttgagat cggcggaggc gcggagctgg ctccaatcgc cggcgcgcaa
      601 agtgaagctg gcgtcggacg tgcggagatt gggtttcttg gtcgtggtgt tgaaaacgcc
      661 gccgggcttg ccgatgccga agagcagggc gttggcgccg cgggcgaact cgacgcgttc
      721 ggtgttgtag gtgtcgacct cgccttcgca cgcgaagaag ttgacggtgc gcgtcgtggg
      781 caagccgcgg gcgcggagtt cggcgcgtcc ggtggagtgc tcgttgccgt tggccacgtc
      841 acccgccatg ccgaacagcg gctcggtgtt gatcgagtaa gcggcgaggt cttcgatcgt
      901 ggtggcgccg atgtcgcgga tgaagtcggc ggtcatgacc gagatcggcg acggcgtgtc
      961 ggcgagcttg gtgttgagac gcgagccgga cgtggtgttt tccgcggcgt aaccggtgtc
     1021 gcgtgcggtg tgcacttcga agaccgaaag cacagcggga tcgtccgctt cgtgttcggc
     1081 tgcaggcgca acggtctgcg cgagcgagcg gctggcgaaa acaaggaggc cgagcgcgag
     1141 aaccgcgcgc agcggcgcgg taaacgggga ggtgaggggc gcgttcatgg tgtggggcgg
     1201 ggcgcggggg ttaggttgaa ggaccgaggc gcgatcggtc gaacgaactt aagaatgggc
     1261 ttgcgccgaa gcagaagtaa cgcgtcggtt tacccgttcc ccggatgccg tccaccgccc
     1321 ccgtcaagct tcaggtgatc gccgatcagg tcggtgtcag ccgcaccacg gtgtcgctcg
     1381 cgctgcgcaa tcacgcgaaa attcccgggg cgacgcgcga gcgaatcaag cgggtggcgg
     1441 aggaactcgg ttatcggcca aatccgctcg tgtcggcgca catggcgttg ttgcggacct
     1501 tgcagccgaa gcacacgggg ttgtgcctgg cgttcgtgtg caaccgatcg ctgcgcgagg
     1561 tgatggcgga cagcctgacg ccgttgcggc tttattatga aggcgcgaag gcgcgtgcgg
     1621 aggagacggg ttacgcgctg caatatttca acctgcggga gcggggtatg acggagcggc
     1681 gtttttcgca gatattgctc gcgcgcggaa ttcacggcgt ggtcatcgcg ccgttgagcg
     1741 agggcagcgg ggtgtcgaac gtggagttgc agtgggaatc gttcgcgttg gcgatgatcg
     1801 agcatacgtt tctcgaaccg cggctgcaca aagtctgcaa cgacgagttt tcgacggtgg
     1861 gccggttgat ccagcgattg ctggactacg ggcacgaacg catcggcatc gcgatgcaca
     1921 gtcggatgga tgaccatgcg aatcatctct ggctcgcggg gttccaggcg ttccaggcgt
     1981 tgacggacaa acggcatcgc gtgccgcact tcatcacgac ggcttggaac aagacggcgt
     2041 ttctcgcgtg gtatcgtaag caccggccgg aggcgatcat cacgatcaat tccgacatcg
     2101 tgggttggct gcgcgaagcg ggctacgacg tgccggagga ggtctcgtgc gcgtcgctct
     2161 actggcgaaa ggagagaaac aacgtgagcg gttattacca gagccacgag ctgatggcgg
     2221 cggcggcggt cgatcatgtc atcggccagc tcaaccgcaa cgagcgcggt ttgccggcga
     2281 gcgagaagac gacgctcatt caggcggaat ggcgcgaagg cgcgacgttg cgccgcaaga
     2341 cg
//
LOCUS       DASPLT010000354         1808 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_209543, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000354 DASPLT010000000
VERSION     DASPLT010000354.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1808)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1808)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1808
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_209543"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..835
                     /locus_tag="VEA63_03705"
     CDS             <1..835
                     /locus_tag="VEA63_03705"
                     /inference="COORDINATES: protein motif:HMM:NF017532.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HYD83123.1"
                     /translation="ALNAEAAAICRGERGPLGGAAQIDVNASDDEVLRHYLCIHFPHK
                     QSAMMRNALFHPPLLRALTAAVGPNLKCMQSMLFIKPSGQPGQAWHQDEDYIPTRDRS
                     LVGAWIALDRATVENGCLWIIPGSHRPGVLWDQHWHGDRRFDCAEESRGFPYTDADAI
                     AVEVEAGSIVCFNGYTLHRSLPNRAAVGTYRRALVNHYMSCESFLPWRRPEANVPMAK
                     LDCRDVVVVAGTDPYAHRGYTDETKTFLRADGKSGCVDWTTSTPKPYADEVASDASAK
                     A"
     gene            1150..>1808
                     /locus_tag="VEA63_03710"
     CDS             1150..>1808
                     /locus_tag="VEA63_03710"
                     /inference="COORDINATES: protein motif:HMM:NF018190.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0016052 - carbohydrate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding protein"
                     /protein_id="HYD83124.1"
                     /translation="MLGFSATADDLVRRPIAFMSVERTKVAPKIDGDLDDLCWRQTRE
                     IGGFQRTAGDRSKPVQFATTARLLFDDTHVYIAFECLEPDVAGLKSTAKSHDDPGIEF
                     DDRVEVFLDLGHDHRNYWELAVNPAGAQFDQAAFYRYHGSRTCDFFPEKNLFWRAKTK
                     IGADRWTVEIAIDVRSLGLMRIEEGTTWGFNLARVRRPAVQHGDELRSRLPGEGAEYS
                     A"
BASE COUNT          331 a          529 c          583 g          365 t
ORIGIN      
        1 cgcgctcaac gcagaggctg cggcgatttg ccggggcgaa cgcggcccgc tgggcggcgc
       61 cgctcagatc gacgtcaatg cgagcgatga cgaagtgctg cgccactatc tttgcattca
      121 ttttccgcac aaacagtcgg ccatgatgcg caacgcgctg tttcatccgc cgctgttgcg
      181 cgcgctcacg gcggcggtcg gccccaacct gaagtgcatg caatcgatgc tgttcatcaa
      241 gccgtcgggc cagccggggc aggcgtggca tcaagacgag gactacatcc cgacgcgcga
      301 tcgctcgctg gtcggggcgt ggatcgcgct cgatcgggcg acagttgaaa acggctgcct
      361 ctggattatt cccgggtctc accgtccggg cgttctctgg gatcaacact ggcacggcga
      421 tcggcgcttc gactgcgcgg aggaatcgcg cggctttccc tacacggatg cggacgcgat
      481 cgccgtcgaa gtggaggcag ggtcgatcgt gtgtttcaac ggttacacgc tgcaccgttc
      541 gttgccgaac cgcgccgctg tgggcacgta tcgccgcgcg ctggtgaacc attacatgag
      601 ttgcgaatcg ttcctgccgt ggcgacggcc ggaagcgaac gtgccgatgg ccaagctcga
      661 ttgtcgtgat gtggtcgtgg tcgccgggac cgacccctac gcgcatcgcg gctacaccga
      721 cgaaacgaaa acatttctcc gcgccgacgg aaaaagcggc tgcgtcgact ggacgacgag
      781 cacgcccaag ccctacgcgg atgaggtcgc gagcgacgct tcggccaagg cctagccgcg
      841 ctcaggctgt tgaaccacgg atggacacgg atgaacacgg attccgatcc ggagttggag
      901 aaccacgaat ggacacgaat tcacacgaat agaggaagag gcatcgatcg agcggtgcat
      961 ttcattcgtg taaattcgtg tccattcgtg gttctacggt ttggtttctc cgtctgctta
     1021 gtccgtgttc atcggtgtcc atccgtggtt tcccagtgaa ctcttccacc gattttccgc
     1081 tttctcatgc atctacattt gttcgttcga cgcactgcga ttcacgcgtg gctgggattt
     1141 tgttctgcgg tgttgggctt tagcgcgact gccgacgatt tagttcgccg gccgattgcg
     1201 ttcatgtcgg tggagcgcac gaaggtcgcg ccgaagatcg acggcgatct cgacgacctg
     1261 tgctggaggc agacgaggga aatcggcggc tttcagcgca cggcgggcga tcggtcgaag
     1321 ccggtgcagt tcgcgacgac cgcgcgactg ctgttcgacg acacgcatgt ttatatcgcg
     1381 ttcgaatgcc tggagcccga cgtcgcgggt ttgaaatcca cggcaaaatc tcatgacgac
     1441 ccgggcatcg agttcgacga tcgcgtggag gtgttcctcg atctcgggca cgatcaccgc
     1501 aattactggg aactcgcggt gaatccggcc ggggcgcagt tcgatcaggc ggcgttttac
     1561 cgctaccacg gttcgcgcac ctgcgatttt tttccggaga aaaatctttt ctggcgcgca
     1621 aagacgaaga tcggcgcgga ccggtggacg gttgagatcg ccatcgacgt tagatcgctc
     1681 gggctaatgc gtatcgaaga aggcacgacg tggggcttca acctcgcccg tgtccgccgg
     1741 cccgcggttc agcacggcga cgagctgcga agccggctgc cgggggaggg cgcggaatac
     1801 agcgcgtg
//
LOCUS       DASPLT010000355         2301 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_229078, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000355 DASPLT010000000
VERSION     DASPLT010000355.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 2301)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2301)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2301
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_229078"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..519)
                     /locus_tag="VEA63_03715"
     CDS             complement(<1..519)
                     /locus_tag="VEA63_03715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family 1"
                     /protein_id="HYD83125.1"
                     /translation="MILFSHPTGNANVRHAALGLHRAGLLGEFWTCVNYRETPLLRRM
                     LPTSLSRQLGRRTFPTELADSIRTFPLREIGRLLAPRAGLRSLVQHEKGPLSVDAVYR
                     SLDRRVSQRLEDERFTAVYAYEDGARESFRVATRHEKLRIYDLPIGYWRASRAILQEE
                     TQREPEWAMTLHG"
     gene            complement(533..1738)
                     /locus_tag="VEA63_03720"
     CDS             complement(533..1738)
                     /locus_tag="VEA63_03720"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016464.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 61 protein"
                     /protein_id="HYD83126.1"
                     /translation="MMSARQLAKAVLFHARAAHIRALAAPRRVAWVRRRLHLPSNETF
                     DPLHDDAPNGVKVTRLDPASTFRRPLPHLPDGNADAHAFFAARTVESLRPTCVAEFEG
                     GIAWGHPTGGVFTNQGRFVPGFTHDPCGEAHHAVWTRLRLPKPKPLAGRVLYLVTPEA
                     TDNFHHWLIDLLPRLGLVQRACYRLADFDRVIVNHASRRYQFSTLERLGIPREKLIAA
                     DTSLFVRAEQLVVPSLKASNQTLPLEDIAFLRRSFLADAQPARPSRRIFLSRADAAFR
                     RLSNEAELHPLLRAHDFEIITPGGLDLSAQARLFADAEIIAGPAGAGFANLVFATAPA
                     RVIEIAQPGWLAAFHWMISARVGLPHTVVLGEGAVMRGVPDSSARERDIVVQPEKLER
                     LLVPTATAV"
     gene            complement(1735..>2301)
                     /locus_tag="VEA63_03725"
     CDS             complement(1735..>2301)
                     /locus_tag="VEA63_03725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD83127.1"
                     /translation="LKVFHRNSDAALIQAPMDKIDEHGRIIGNNLEAQKHVVEHLKEI
                     YRQHDVDFFYPTSALAFSRTYLECVLPLDFSDGLPLWSDTRLSIVAPYFGRVITLPDR
                     LTEWRRHTGCDSIRTRSRHLQIRQTLMRTRVFNSFCRRYGLRTISPWRNRRFYLQLLR
                     YSLPEPAYRLFHSRFRSRGTQQIESPSE"
BASE COUNT          425 a          644 c          857 g          375 t
ORIGIN      
        1 gccatgcagc gtcatcgccc attcgggttc gcgttgcgtt tcctcctgga gaatcgcgcg
       61 cgacgcgcgc cagtagccga tgggtagatc gtagatgcgc agcttttcgt gacgtgtggc
      121 gacgcggaaa ctttcgcgcg caccatcctc gtaagcgtag acggcggtga agcgctcgtc
      181 ttcgaggcgt tgactaacgc gccggtcgag tgagcggtaa acggcgtcca cgctgagcgg
      241 acctttctcg tgttgcacga gcgagcgaag gcccgcgcgc ggtgcgagca gacggccgat
      301 ctcgcgtagc ggaaaggtgc ggatgctgtc ggcgagttcg gtgggaaacg tgcggcggcc
      361 gagttggcgg gagagcgagg tcggcagcat gcgccgcagg agcggcgttt cgcggtagtt
      421 cacgcaggtc cagaattcgc cgagcaagcc cgcgcggtgc aggccaagcg cagcgtggcg
      481 aacgttcgcg tttccggtgg gatgagagaa aagaatcata ggaatgcggc ggttaaaccg
      541 cggtcgcggt gggaacgagg aggcgctcga gtttctccgg ctggaccacg atatcgcgtt
      601 ctcgcgcgga ggagtccggc acaccgcgca tcaccgcgcc ttcgccgagc acgacggtgt
      661 gcggcagccc gacgcgcgcc gagatcatcc aatgaaatgc ggcgagccaa ccgggctgtg
      721 cgatttcgat cacgcgcgcg ggcgccgtgg cgaaaacgag attggcaaaa ccggcgccgg
      781 cagggccggc gatgatctcg gcgtcggcga agagccgcgc ttgtgcgctg aggtcgaggc
      841 cgccgggtgt gatgatttcg aagtcgtgcg cgcgaagcaa cggatgcaac tcggcttcgt
      901 tgctgagccg gcgaaacgcc gcgtcggcgc gactgagaaa aatccgccgc gaaggccgcg
      961 cgggctgcgc gtcggcgaga aagctgcgcc gaagaaaagc gatgtcttcc agcggaagcg
     1021 tttggttgct cgctttcagt gacggcacaa cgagctgctc cgcgcgcacg aagagcgagg
     1081 tgtcggcggc gatgagtttt tcgcgtggga tgccgagtcg ttcgagcgtc gagaactggt
     1141 agcggcggct ggcatggttg acgatgacgc gatcgaagtc ggcaagccga tagcaggcgc
     1201 gttgcacgag cccgagtcgc gggagcagat cgatcagcca gtggtgaaag ttatccgtgg
     1261 cctcgggcgt gaccagatac agcacgcggc ccgcgagcgg cttcggtttg ggcaggcgca
     1321 gccgggtcca cacggcgtga tgcgcttcgc cgcagggatc gtgcgtgaag ccgggcacga
     1381 agcgtccttg gttcgtgaat acgccgccgg ttggatgccc ccaggcgatg ccaccctcga
     1441 actcggcgac gcaagtcggg cgcagcgatt cgaccgtgcg ggcggcgaag aacgcgtgcg
     1501 catcggcgtt gccatcggga aggtggggaa gcgggcggcg aaacgtgctc gccggatcga
     1561 gtcgcgtgac cttcacaccg ttcggcgcgt cgtcatgcaa aggatcgaac gtttcgtttg
     1621 acggcagatg caaacggcgg cgcacccacg cgacgcggcg cggagcggcg agcgcgcgga
     1681 tgtgcgcggc gcgcgcgtga aacaggacgg ctttggcgag ttgacgggcc gacatcattc
     1741 ggagggcgac tcgatttgct gcgtgccgcg cgagcggaag cgcgaatgaa agaggcgata
     1801 cgcgggctcg ggcagcgagt agcgcagcaa ctggagatag aagcggcggt ttctccaggg
     1861 agagatcgtg cggaggccgt agcggcggca gaagctgtta aacacgcgcg tgcgcatgag
     1921 cgtttgacga atctgcagat gccgcgaacg cgtgcggatc gagtcgcatc ccgtatggcg
     1981 gcgccattcg gtgagtcggt cgggcagcgt gatcacgcgg ccgaagtagg gcgcaacgat
     2041 gctgagacgc gtgtcgctcc acagcggcag gccgtcggag aaatcgagcg gcagcacgca
     2101 ttcgaggtaa gtgcgcgaga acgcgagcgc gctcgtcgga taaaagaaat ccacgtcgtg
     2161 ctgccggtaa atttccttga ggtgctcgac gacgtgtttc tgcgcctcga gattgttgcc
     2221 gatgatccgg ccgtgctcgt cgatcttgtc catcggcgcc tggatgagcg cggcgtcgga
     2281 gttgcggtga aagaccttca g
//
LOCUS       DASPLT010000356         1432 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_291760, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000356 DASPLT010000000
VERSION     DASPLT010000356.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1432)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1432)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1432
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_291760"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..300)
                     /locus_tag="VEA63_03730"
     CDS             complement(<1..300)
                     /locus_tag="VEA63_03730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007362742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HYD83128.1"
                     /translation="MLPFPVELKPGLPIAEQVIFAVKRAVVTGQLKPGVPFPSVRVIS
                     QELKINPNTAHKIVAALVTQGVLLTTPAVGSIVATPEAGGKRERAELLGDELERVV"
     gene            628..>1432
                     /locus_tag="VEA63_03735"
     CDS             628..>1432
                     /locus_tag="VEA63_03735"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF014502.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009341 - beta-galactosidase complex
                     [Evidence IEA];
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HYD83129.1"
                     /translation="MKKLFHGVCYYPELWPETEIDRDIAEMKRVGINFVRMGEFAWSR
                     IEPEEGRISLDFFVGVMDRLHAAEIGVVLCTPTPTPPVWLTHGHPERCFVDADGNVMG
                     HGARQHASYENPDVRAACLRIVEACSAALGKHPALVAWQIDNEFKCHVAEDFNPSAVA
                     HWHGWLKNKYGTIERLNAAWGADIWSETYQRFEQVPAPRKTPFLHNASLSTAYRMFSR
                     ESIAAFMDAQCAVLRKHSAAPITHNFGLEFSVNFERMSEGLDFVSFDDYP"
BASE COUNT          253 a          368 c          529 g          282 t
ORIGIN      
        1 gacgacgcgc tcgagttcgt cgccgagcag ctccgcgcgc tcgcgtttgc cgccggcctc
       61 gggagtggcg acgatgctgc cgacggcggg cgtggtgagg agaacccctt gggtcacgag
      121 cgcggcgacg atcttgtgcg cggtgttggg gttgatcttg agctcctggc tgatcacgcg
      181 aacggaagga aaagggacgc cgggtttcag ctggcccgtg acgacggcgc gcttgacggc
      241 gaaaatcacc tgttcggcga tcggcagacc gggcttcagc tcgacgggga aggggagcat
      301 gctgtattac tcacactagg acagatggga cggcaagatt ttatttctga tcggttgatt
      361 gtcgaaggag agcgcgaacg agcgtgaggg cggatgcccg ggcggcgggg tgcggcggcg
      421 agtctggctg catacgcgca gcgttttgta gcgaaacgcg tgagcgtttc gtccgtgcga
      481 gagacgaaaa gctcacgctt ttcgctacgt gtgaattttt ccgggcggag ggtgcgagtg
      541 ccgaactcgg cgcggtcatg cgcggctgcg tggtggctat ttgtgcgaaa tcggatcaat
      601 tcggcggtgg gacattttct gtttcacatg aaaaaacttt ttcacggcgt ttgttactac
      661 ccggagcttt ggccggagac cgagatcgat cgcgacatcg cggagatgaa gcgcgtcggg
      721 atcaatttcg tgcggatggg cgagttcgcc tggtcgcgga tcgagccgga ggaagggcgg
      781 atctcgctcg atttcttcgt gggcgtgatg gaccggctgc acgcggcgga aatcggcgtc
      841 gtgctatgca cgccgacgcc gacgccgccg gtgtggctga cgcacgggca cccggagcgg
      901 tgcttcgtcg atgcggacgg caatgtgatg gggcacggcg cgcggcagca tgcgtcgtat
      961 gaaaacccgg acgtgcgcgc ggcgtgtctg cgaatcgtcg aggcgtgcag cgcggcgctg
     1021 gggaaacacc cggcgctggt ggcgtggcag atcgacaacg agttcaagtg tcacgttgcg
     1081 gaggatttca atccgtcggc agtcgcgcac tggcacgggt ggttgaaaaa caaatacggc
     1141 acgatcgagc ggttgaatgc ggcgtggggg gcggacattt ggagcgagac ctatcagcgc
     1201 ttcgagcagg tgccggcgcc acgcaagacg ccgttcctgc acaacgcgtc gctgagcacg
     1261 gcctatcgga tgttttcgcg ggagagcatc gcggcgttca tggacgcgca gtgcgcggtc
     1321 ttgcggaagc actcggcggc gccgatcacg cataatttcg gtttggagtt ttccgtgaac
     1381 ttcgagcgga tgagcgaagg gttggatttc gtgtcgttcg acgattatcc gg
//
LOCUS       DASPLT010000357         1896 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_291796, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000357 DASPLT010000000
VERSION     DASPLT010000357.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1896)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1896)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1896
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_291796"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1896
                     /locus_tag="VEA63_03740"
     CDS             <1..>1896
                     /locus_tag="VEA63_03740"
                     /inference="COORDINATES: protein motif:HMM:NF012722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HYD83130.1"
                     /translation="TANAAQTTVGDALFRMAEHRVPLPPGGLPKLRLTDQLRELSPEE
                     AARSYPVQLSGVITWTRPDAEFFFVRDVSGGICVYHPPGMTQSLAVGRKVELTGVSAS
                     GRFTPVVYASAVQPLGTIDLPEARVITLEQALTGVEEAQWVTMSGYVRAAERDGRWMR
                     LDLTTFGGEFHAMLPPNAQWASLPGAVVRLRGVCGAIANEKRQLTGIQLWVASSRFVE
                     IEEAVPEDPFAVERRSIASLRQFGTLQVLNRRVRVAGVVVHHEPGRLVNIQGGNEGLL
                     VLSRDTQPLQPGDRIEAVGFPGRENSRAVLREAVYRRIESGAEPEPVVVDDIGVIDVE
                     LDGRLVRVEGLLLDVGAREREVELINQQGGVVFRAQLRLPDGDAEVRWEPGSKLVLTG
                     VYEVSYDEYRRPHEVRLKLRTPDDVAVLRQPSWWTVKKVLALTGVLGVAVVLGFGWVL
                     ALRRRVREQTSVIRERLENERAARLEAALARASKLESLGVLAGGIAHDFNNLLTVILG
                     NVSLAKMDARIDSDTVHCLNESERAAQRARDLTQQLLTFAKGGEPMRAATRLPDIVRE
                     AAQFALHGSNVRSEFEIASDLWPADVDRGQIGQVVHNIVINANQAMPAGGLVRIALQN
                     EEVGEARS"
BASE COUNT          290 a          495 c          773 g          338 t
ORIGIN      
        1 acggcgaacg cggcgcagac gacggtgggt gacgcgttgt tcagaatggc ggagcatcgg
       61 gtgccgctgc cgccaggagg tttgccgaag ctgcgcctca cggatcaatt gcgcgagttg
      121 tcgccggagg aagcggcgcg aagttatccg gtgcaactct cgggtgtgat cacgtggacg
      181 cggccggatg cggagttttt cttcgttcgc gatgtgagcg gcggcatctg tgtttaccat
      241 ccgccgggga tgactcaatc gctggcggtc gggcgcaagg tggagctcac gggcgtgtcg
      301 gcgtcggggc ggtttacgcc ggtggtgtat gcatcggcgg tgcaacccct cgggacgatc
      361 gatctgccgg aggcgcgggt catcacgctg gagcaggcgt tgacgggcgt ggaggaagcg
      421 cagtgggtga cgatgagcgg ctatgtgcgc gcggcggagc gcgacgggcg atggatgcgg
      481 ctggatctga cgacgttcgg cggggagttt cacgcgatgc tgccgccgaa tgcgcagtgg
      541 gcgagcttgc cgggtgcggt ggtgcgcctg cgcggcgtgt gcggcgcgat cgcgaatgag
      601 aagcggcagt tgacgggcat tcagctttgg gtggcgtcgt cgcgttttgt cgagatcgag
      661 gaggcggtgc cggaggatcc gtttgcggtg gagcggcgct cgatcgcgag cttgcggcaa
      721 ttcgggacgt tgcaggtgtt gaaccggcgc gtgcgcgtcg cgggtgtggt ggtgcatcac
      781 gagccggggc ggctggtgaa cattcaaggc ggcaacgaag gcttgttggt gttgagccgc
      841 gacacgcagc cgctgcaacc gggcgatcgg atcgaggcgg tgggttttcc cggtcgcgag
      901 aacagccgcg cggtgctgcg cgaagcggtt tatcgccgca tcgaaagcgg cgcggagccg
      961 gagccggtgg tggtcgacga catcggcgtg atcgacgtgg agctcgacgg gcgcctggtg
     1021 cgcgtcgagg gcttgctgct ggacgtgggc gcgcgcgagc gcgaagtcga gttgatcaat
     1081 caacaaggcg gggtggtgtt tcgcgcgcag ttgcgtctgc ccgacggcga cgcggaggtg
     1141 cggtgggaac cgggttcgaa actcgtgctg acgggcgtgt atgaggtttc ctacgacgag
     1201 tatcgccggc cgcacgaagt ccgactcaag ctgcgcacgc cggacgatgt cgcggtgttg
     1261 cgccagccgt cgtggtggac ggtgaagaaa gtgctggcgc tgacgggcgt gctcggcgtg
     1321 gcggtggtgc tcggtttcgg ctgggtgctg gcgttgcgcc ggcgcgtgcg cgagcagacg
     1381 agcgtgattc gcgaacggct cgagaacgag cgggcggcgc ggctcgaggc ggcgctcgca
     1441 cgggcgtcga aactcgagtc actcggcgtg ctcgcgggcg gaatcgcgca cgatttcaac
     1501 aatttgctca cggtcattct cggcaatgtt tcgctggcga agatggatgc gcgcatcgat
     1561 tcggacacgg tgcactgtct gaacgagagc gagcgggcgg cgcagcgggc gcgggatttg
     1621 acgcagcagt tgttgacgtt cgcgaaaggc ggcgagccga tgcgcgcggc gacgcggttg
     1681 ccggacatcg tgcgcgaagc ggcgcagttc gcgttgcacg gatcgaacgt gcgttcggag
     1741 ttcgagatcg cttcggatct ctggccggcg gatgtcgatc gcgggcagat cgggcaagtc
     1801 gtgcacaaca tcgtcatcaa cgcgaatcag gcgatgccgg cgggcgggct ggtgcggatc
     1861 gcgttgcaga acgaagaagt gggcgaggcg cgctcg
//
LOCUS       DASPLT010000358         1014 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_44309, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000358 DASPLT010000000
VERSION     DASPLT010000358.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1014)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1014)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1014
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_44309"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            195..>1014
                     /locus_tag="VEA63_03745"
     CDS             195..>1014
                     /locus_tag="VEA63_03745"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RecQ family ATP-dependent DNA helicase"
                     /protein_id="HYD83131.1"
                     /translation="MPVTTPNLEQLLKRTFGYGTFRPLQRDIIEATLGGRDVFALLPT
                     GGGKSLCFQLPALARPGLTVVVSPLIALMKDQVDQLQASGVAATFLNSTLGADESRAR
                     LRGLHRGDYKLLYAAPERLMLDGWHENLKAWNVACLAIDEAHCVSEWGHDFRPEYRQL
                     AKLRAALPEVPVMALTATATSRVREDIIKHLKLHDPATFVASFNRPNLTYRVVPKDQP
                     AKQIIDFVRKREHESGIIYCASRATAEPVAESLAGRGFSARPYHAGLTAEERATN"
BASE COUNT          167 a          389 c          263 g          195 t
ORIGIN      
        1 tccgtgagct ctgtgtcatc gttccgtgcc ctctgtgttc catgcctggc gtttttcggt
       61 ttgatcgctc ccggcgttgt ccatccttct ccgccgtatc ttccccagta tcataggatg
      121 gggcgcactc tccggcaccg cgcagaattt ttccgtgcgc tcgccgtctt ctccgcgcta
      181 catcaccgcg tccaatgccc gtcaccacgc ccaaccttga gcaactcctg aagcgcacct
      241 tcggctacgg cacgtttcgc ccgctccagc gcgacatcat cgaggcgacg ctcggcggtc
      301 gtgacgtttt tgcgctgctt cccaccggcg gcggcaaatc gctgtgtttc caacttcccg
      361 cgctcgcccg ccccggtctc accgtcgtcg tctcgccgct gatcgcgttg atgaaagacc
      421 aggtcgacca gctccaggcc agcggcgtcg cggccacgtt tctcaactcc acgctcggcg
      481 ccgacgaatc ccgcgcgcgt cttcgcggcc tgcatcgcgg cgactacaag ctcctctacg
      541 ccgcgcccga gcggctcatg ctcgatggct ggcacgaaaa cctgaaggca tggaacgtcg
      601 cctgcctcgc gatcgacgaa gcgcactgcg tttccgaatg gggccacgat tttcgccccg
      661 aatatcggca gctcgcaaaa ctccgcgccg ctctccccga agttcccgtg atggcgctga
      721 ccgcaaccgc aacgagccgc gtgcgcgagg acatcatcaa gcatctcaag ctgcacgacc
      781 ccgccacctt cgtcgcgagc ttcaaccgcc ccaatctcac ctaccgcgtc gttccaaaag
      841 atcagccggc aaaacaaatc atcgatttcg tccgcaagcg cgaacacgag agcggtatta
      901 tctactgcgc cagccgcgcc accgccgaac ccgtcgccga gtcgctcgcc ggccgcggtt
      961 tctccgcgcg cccctatcac gccggcctca ccgccgagga gcgcgcgacg aatc
//
LOCUS       DASPLT010000359         1061 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_189107, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000359 DASPLT010000000
VERSION     DASPLT010000359.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1061)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1061)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1061
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_189107"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..323)
                     /locus_tag="VEA63_03750"
     CDS             complement(<1..323)
                     /locus_tag="VEA63_03750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD83132.1"
                     /translation="MAAIDQLLRTMLEKGGSDLHLTVGLPPKARISGALTPIAEAPLT
                     ATQMESLLKEILPERRWQDYLERKDLDLAHEIPGVARFRGNYLYNHWGQGAVFRQIPA
                     KILSF"
     gene            complement(436..>1061)
                     /locus_tag="VEA63_03755"
     CDS             complement(436..>1061)
                     /locus_tag="VEA63_03755"
                     /inference="COORDINATES: protein motif:HMM:NF012652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HYD83133.1"
                     /translation="SMVAYLNETRTDHIITVEDPIEVVQPAKGCNVTQREVGPHTKSF
                     FTALKGALREDPDIIVIGELRDLETIEMAIAASETGHLVIGTMHTSDAATTLNRLLDV
                     FPPAQQAQIRASVAESLRGIVCQRLLPAIDGGLTLACEILINNTAISNSIREGKTTGL
                     RNTMETGVREGMCLMDNVVFGLYTDGTVAADTALASINNRVLRAKIR"
BASE COUNT          174 a          268 c          398 g          221 t
ORIGIN      
        1 tcgaacgaga ggatcttcgc ggggatctgg cggaaaacgg cgccctggcc ccagtgattg
       61 tagaggtagt ttccgcggaa gcgggcgacg ccgggaatct cgtgggcgag gtcgaggtcc
      121 ttgcgttcga ggtaatcctg ccaacggcgc tcggggagga tttccttgag gagcgattcc
      181 atctgcgtcg cggtgagcgg cgcttcggcg atgggcgtga gcgcgccgga gatgcgggct
      241 tttggtggca gcccgaccgt gagatggagg tcggaaccgc ctttctcgag cattgtgcgg
      301 aggagttgat cgatggcggc cattgggcgg aaacctgaaa cctgaaatgc tgaaacctga
      361 aatttgaagg gctgagggct ggaaaattcg gggcgcggtg cggacgcctc ggagaggcgt
      421 ccctaccagg cttctctacc ggattttggc gcggaggacg cggttgttga tgctggcgag
      481 ggcggtgtcg gcggcgacgg tgccgtcggt gtagaggccg aagacgacgt tatccatcag
      541 gcacattcct tcgcgcacgc ccgtttccat cgtgttgcgc aggccggtag ttttgccttc
      601 gcggatcgaa ttcgagatgg cggtgttgtt gatcaggatt tcgcacgcga gggtgagtcc
      661 gccgtcgatt gcgggaagca ggcgctggca gacgatcccg cgcagactct cggcgacgct
      721 ggcgcggatt tgtgcctgtt gcgccggcgg aaagacgtcg agcaggcgat tgagcgtggt
      781 cgcggcgtcg ctcgtgtgca tcgtgccgat gacgagatgg ccggtttcgc tcgcggcgat
      841 cgccatctcg atggtctcga ggtcgcggag ttcgccgatg acgatgatgt cggggtcttc
      901 gcggagggcg cctttgagcg cggtgaaaaa cgatttcgtg tggggaccga cctcgcgctg
      961 cgtgacgttg cagcccttcg caggttgaac gacctcgatg ggatcttcga cagtgatgat
     1021 gtgatcggtg cgcgtctcgt tgaggtaggc gaccatcgaa g
//
LOCUS       DASPLT010000360         1261 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_270662, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000360 DASPLT010000000
VERSION     DASPLT010000360.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1261)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1261)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1261
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_270662"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1261
                     /locus_tag="VEA63_03760"
     CDS             <1..>1261
                     /locus_tag="VEA63_03760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD83134.1"
                     /translation="YAMNQFRNGYMFAPSLAWQLSPAHKLVIKAEFMQNRESITSGLP
                     IDPSVGSDDYARIARGLPRDWSFGTEDHDFRHRATERVTMELLSDLSDNVTSRLQLML
                     NHVVREDTGGTGAAIAGIGVTRNPSTGKYEPGVTWSLDQTGATAVATATSTPVPAPDT
                     WVYSRSTNAVDLFYSEAHLRNDYAFKFNTDWLKSTTIAGLAANFSKVQFKVYGTASRP
                     SVPNNNLGAITYPPWNYPAPVLLNDNRRGQQEDAQIFVYESASLLNDRLILSGGVSRF
                     FGELSRRYNAALEPTILYPTYNLSTTAKTFGVVVKPIAPVSLFSGYNTTGGTMPSSLN
                     PGTYAPSFRAAAGTQHEYGVKVAALDDRLTASFSHFDISQENYAVPNSEYYTLIALGR
                     TEEAAALPNPLYLNLDSKGWEVESTFAV"
BASE COUNT          240 a          483 c          325 g          213 t
ORIGIN      
        1 ctacgcgatg aaccagtttc gcaacggtta catgttcgcg ccgtccctcg cgtggcagct
       61 cagccccgcg cacaaactcg tcatcaaggc ggagttcatg cagaatcgcg aaagcatcac
      121 gagcggcctg ccgatcgacc ccagcgtcgg tagcgacgac tatgcccgca tcgcccgcgg
      181 cctcccgcgc gactggtcgt tcggcacgga ggatcacgac ttccgccatc gcgcgaccga
      241 gcgcgtcacg atggaactcc tctccgacct cagcgacaac gtcacctcgc ggctgcagtt
      301 gatgctcaat catgtcgtcc gcgaggacac cggtggcacc ggcgcggcga tcgccggcat
      361 cggcgtcacc cgcaatccca gcaccggcaa atacgagccg ggcgtcacct ggtcgctcga
      421 ccaaaccggc gcgaccgctg tcgccaccgc cacgagcacg cccgtgcccg cacccgacac
      481 ctgggtctat tcgcgcagca caaacgccgt cgaccttttc tactccgagg ctcacctgcg
      541 caacgactac gccttcaagt tcaacaccga ctggctcaaa tccacgacga tcgccggcct
      601 cgccgcgaat ttctccaagg ttcaattcaa ggtttacggc accgcctccc ggcccagcgt
      661 cccgaataac aacctcggcg ccatcacgta tccgccttgg aactaccccg ctccggtgct
      721 gctgaacgac aaccgccgcg gtcagcagga ggacgcgcag atcttcgtct atgaatccgc
      781 gagtctcctg aacgatcgcc tcatcctgtc cggcggcgtt tcgcgtttct tcggcgagct
      841 ctcccgccgt tacaacgccg cgctcgaacc cacgattctc tatcccacct acaacctctc
      901 cacgacggcg aaaaccttcg gcgtggtcgt aaaaccgatc gcgcccgtgt cgctcttttc
      961 cggctacaac accaccggcg gcaccatgcc gagttcgctc aaccccggca cttacgctcc
     1021 gagtttccgc gccgccgccg gcacccagca cgaatacggc gtcaaggtcg ccgcgctcga
     1081 cgatcggctc accgcctcgt tctcgcattt cgacatctcg caggagaact acgcggttcc
     1141 gaacagcgaa tactacacgc tcatcgccct cggccgcacc gaggaagccg cggcgctgcc
     1201 caacccgctc tatctcaacc tcgactcgaa gggctgggag gtcgaaagca cgttcgccgt
     1261 g
//
LOCUS       DASPLT010000361         1191 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_270675, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000361 DASPLT010000000
VERSION     DASPLT010000361.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1191)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1191)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1191
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_270675"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            147..>1191
                     /locus_tag="VEA63_03765"
     CDS             147..>1191
                     /locus_tag="VEA63_03765"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HYD83135.1"
                     /translation="MFTLVTACMNREQHLRKTLHSWLKLPQIAEVVIVDWSNNRPLDS
                     LRAVDERVKIVRVEGEPRWILSYAYNVGVVHATQPLILKCDADCIPRPEVVACIPEAS
                     CFYAGNWRSGRPVGKPSVNGQTIFLKSHFEAVNGYSEVIRTYGRDDEDFYDRLIAAGF
                     ERREIAPDLLDFLDHSDAERTANQFDADSRLPIEKRIVRDLLYCEMRNYYLAQRMPWG
                     ASQRRATFQEIGADDRLTVLRRDRASEIVIPGEIEREAHLFALRFVTTQLAGISLEAA
                     ESLDERACLILIASRSKPVAHLVGAKRVVAASPSGSPRANGVRGRTANALAPSAASTA
                     VARVRTQPLSSAAQ"
BASE COUNT          219 a          331 c          394 g          247 t
ORIGIN      
        1 cacggcgaaa ttctcgcgga ggtcacaaca attttgggcg agttggctcc gacggatccg
       61 cgttacggtg cgctgcgcga cggcgcggcg gactccgccg tggcgagcta ggcaacgcag
      121 tcaaagcacc aagttactac tcaatcatgt tcactctcgt cactgcctgc atgaaccgtg
      181 agcagcatct gcggaagacg ctgcactcgt ggttgaagct gccgcaaatc gcggaggtgg
      241 tgatcgtcga ctggtcaaac aaccgaccgc tcgattcgtt gcgcgcggtc gacgagcgcg
      301 tgaagattgt gcgggtggag ggtgagccgc ggtggatttt gtcgtatgcc tataacgtcg
      361 gcgtcgtcca tgcgactcag ccgctgattt tgaaatgcga cgcggactgt attccgcggc
      421 cagaagtcgt tgcctgtatt ccggaagcaa gctgcttcta tgcgggtaac tggcgttcgg
      481 gtcgtccggt cggcaaaccc tcggtgaacg gtcagaccat cttcttgaag agccacttcg
      541 aggcggtgaa tggctattcg gaagtgattc gcacgtatgg tcgcgacgat gaagattttt
      601 atgaccgctt aattgccgcg ggattcgagc gccgcgagat cgctccggac ctgctcgatt
      661 ttctggacca ctccgatgcc gagcgaaccg cgaatcaatt cgatgcggac agccggctac
      721 cgatcgaaaa acggatcgtg cgcgatctgc tttactgcga gatgcggaat tactacctcg
      781 cccaacggat gccgtggggc gcgagccagc ggcgcgcgac gtttcaagag atcggtgctg
      841 atgaccggct gacggtgctg cgtcgagacc gggcctcgga gattgtgatt cccggcgaaa
      901 tagagcggga agcgcatttg ttcgcgctgc ggtttgtgac gactcaactc gctgggattt
      961 cgttggaggc ggcggagagc ctcgatgaac gggcgtgcct gatcctcatc gcttcacgca
     1021 gcaagccggt cgcgcatctc gtcggcgcga aacgggtcgt tgcggcgtca cccagcggat
     1081 cgccacgggc gaatggtgtt cgtggtcgga cggcgaatgc gttggctccg agcgctgcgt
     1141 cgactgcggt ggcgcgggtg cggactcagc ctctcagctc tgccgcgcag t
//
LOCUS       DASPLT010000362         1500 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_291816, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000362 DASPLT010000000
VERSION     DASPLT010000362.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1500)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1500)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1500
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_291816"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            13..>1500
                     /locus_tag="VEA63_03770"
     CDS             13..>1500
                     /locus_tag="VEA63_03770"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HYD83136.1"
                     /translation="MSKTLSGIITSWNSGAERIFGYSASEAVGRSITMLLPPERLSEE
                     EDILRRVKQGERIDHFETLRRRKDGAIIDVSVTISPLREPDGTIVGASKVARDISGQK
                     RSNRADVLLAAIVSSSDDAIVSKNLDGIITSWNQGAERLFGYTPEEAIGRPVLMLVPA
                     DRKDEEPRILERMRRGERVDHFETVRVRKNGEHFHVSLTISPLRDANGIIVGASKIAR
                     DITELKRISAEREMLLESERVARAQAEHANRMKDEFLATVSHELRTPLNAIVAWTQVL
                     KEGGGPLAEIEQGLEVIERNARIQAQLIDDLLDLGRIASGKMTLETEWLDLALIVQEA
                     IRSVQHAADAKQISLKTVLNTVRGGMVGDKQRLQQVVWNLLSNAIKFTPKHGTVLVAV
                     NRVNSHVEISVSDNGRGIAPDFLPHVFERFRQADASTTRQFGGLGIGLSLVRQFVELH
                     GGLVRAESPGLGQGATFTVSLPVVISRPEALPNKAGPRLADGDIAP"
BASE COUNT          293 a          509 c          452 g          246 t
ORIGIN      
        1 gacgacgcga ttgtgagcaa aaccctgagc ggcatcatca ccagctggaa tagcggggcc
       61 gagcggatct ttggttacag cgcgagcgaa gccgtgggtc gctcgatcac gatgttgctg
      121 ccgccggagc ggctttccga agaggaggac atccttcggc gcgtcaaaca aggcgagcgc
      181 atcgaccact ttgaaaccct gcgccgccgc aaagacggtg ccatcatcga cgtctccgtc
      241 accatctcgc ctcttcgcga gccggatggc accattgtcg gtgcgtcgaa agtcgcgcgc
      301 gatatcagcg gtcaaaaacg cagcaaccgc gccgacgttc tgctcgcggc gatcgtcagc
      361 tcttccgacg acgcgatcgt gagcaagaac ctcgacggca tcatcaccag ttggaatcaa
      421 ggtgcggaac gcctctttgg ctacacgccc gaagaagcca tcgggcgtcc ggtgttgatg
      481 ctcgttcccg ccgaccgaaa ggacgaggaa ccgcggatcc tcgagcgcat gcgccgcggc
      541 gaacgcgtcg atcacttcga aaccgtccgc gtccgcaaga acggcgaaca cttccacgtc
      601 tcattgacca tctcgccgct gcgcgacgcc aacggcatca tcgtgggcgc ctcgaagatc
      661 gcgcgcgaca tcaccgaact caaacgcatt tccgccgagc gcgagatgct cctcgaaagc
      721 gaacgcgtcg cgcgtgcgca ggccgagcac gccaatcgca tgaaggacga gtttctcgcg
      781 accgtatccc acgagctgcg cacgccgctc aacgcgatcg tcgcatggac gcaggtgctc
      841 aaggaaggcg gcggcccgct cgcggagatc gagcaaggtc tcgaagtcat cgagcgcaat
      901 gcccgcatcc aggcgcaact catcgacgac cttctcgacc tcgggcgtat cgcctcgggc
      961 aagatgacgc tcgaaaccga gtggctcgac ctcgcgctga tcgtgcagga ggcgatccgg
     1021 tccgtgcaac acgccgccga cgccaagcag atcagcctca agaccgttct caacacggtc
     1081 cgcggcggaa tggtcggcga caagcagcgc cttcagcagg tggtgtggaa cctgttgtcg
     1141 aacgccatca agttcacccc gaagcacggc accgtgctcg tcgcggtcaa ccgcgtgaac
     1201 tcgcacgtgg agatttccgt ctccgacaat ggccgcggca tcgcgccgga ttttctgccg
     1261 catgtcttcg agcgtttccg ccaggctgac gcgtcgacca cgcgtcaatt cggcggcctc
     1321 ggcatcggac tctcgcttgt gcggcaattc gtcgagctgc atggcggact cgtccgcgcc
     1381 gaaagcccgg gcctcggcca gggcgcgacg ttcaccgttt ctctgcccgt cgtcatctca
     1441 cgccccgagg ctctgccgaa caaggcgggg ccgcgcctgg ccgacggcga tatcgcacct
//
LOCUS       DASPLT010000363         1106 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_4880, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000363 DASPLT010000000
VERSION     DASPLT010000363.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1106)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1106)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1106
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4880"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1044)
                     /locus_tag="VEA63_03775"
     CDS             complement(<1..1044)
                     /locus_tag="VEA63_03775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD83137.1"
                     /translation="MKAVLERDCSFREAKQVLEREFVRSLDGASWKWAKEELAEISNA
                     GLAINGKVTRKAGGEIAVWGGDCATCPKRTGNIEGCEGSPNVCTDLDCFGRKLAKAGE
                     IRLREAEASGQRVITRKEWERVRYNRAYVEPEKEVYEVSWDKPIGEVLKKAKIEPPMV
                     VVDSDQGVLEMVDVARAKDLLKDAGVLKTESRRMGESDKAARAKAVLEARVAEAKTTA
                     IVEKVEKMGEPDLAMWRRLAHAAAMSCWHDSAREIAKRRGLAEKGKSPDDVLAKATET
                     FTEAECFGFLTEVLVARGLRGEWHVREDAKAVQQELKLDLGAVEKRVKAELEAKRKEK
                     ESKSKAREATAKKK"
BASE COUNT          170 a          459 c          236 g          241 t
ORIGIN      
        1 ctttttcttc gcggtcgcct cccgcgcttt gctcttcgat tccttctcct tccgcttcgc
       61 ctcgagctca gccttcaccc gcttctccac cgcgcccaaa tccagcttca gctcctgctg
      121 caccgccttc gcgtcctccc gcacgtgcca ttccccgcgc aaaccgcgcg ccaccaaaac
      181 ctccgtcagg aatccgaagc actccgcctc cgtgaacgtt tccgtggcct tcgccagcac
      241 gtcgtccggc gatttccctt tttccgcgag gccccggcgc ttcgcgatct cccgcgccga
      301 gtcgtgccag caactcatcg cggccgcgtg cgccagccgg cgccacatcg ccaaatccgg
      361 ctcgcccatc ttctcgacct tttccacgat cgccgtcgtc ttcgcctccg cgacccgcgc
      421 ttccaaaacc gctttcgccc gcgccgcttt gtccgactct cccattcgtc gcgactccgt
      481 tttcaaaacg ccggcgtctt ttaaaaggtc cttcgcccgc gccacatcga ccatttccaa
      541 aaccccttgg tccgaatcca cgaccaccat cggcggctcg attttcgctt tcttcaggac
      601 ctcgccgatc ggcttgtccc aggaaacctc gtaaacctcc ttctcaggct ccacataagc
      661 gcggttgtaa cgcacccgct cccattcctt ccgcgtgatc acccgctgcc cgctcgcttc
      721 cgcctcccgc aaacggatct caccggcctt ggcgagcttc cggccgaaac agtccaggtc
      781 agtgcaaacg tttggactcc cttcgcaccc ctcgatattc ccggtccgct tcgggcacgt
      841 cgcgcaatcg ccaccccaga ccgcaatctc gcctccggct ttccgtgtga ccttcccatt
      901 gatcgcgaga cccgcgttcg aaatctccgc cagctcctct tttgcccact tccaggaagc
      961 cccatcgagc gaccgcacaa actcccgctc gagcacctgc ttcgcctcgc gaaacgaaca
     1021 atcacgctcc aggacggcct tcacgaactc cttttggatt ttctcaggca actgcgccgc
     1081 caggcccgcg atcgtcgccg ggatct
//
LOCUS       DASPLT010000364         1011 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_168704, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000364 DASPLT010000000
VERSION     DASPLT010000364.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1011)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1011)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1011
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_168704"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1011)
                     /gene="aroC"
                     /locus_tag="VEA63_03780"
     CDS             complement(<1..>1011)
                     /gene="aroC"
                     /locus_tag="VEA63_03780"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HYD83138.1"
                     /translation="MHLRYTTAGESHGPALVVVIEGVPAGLALEVGRDVDPELRRRQG
                     GYGRGDRMKIEQDRAEFLSGVRLGETLGSPIALVIWNRDWENWQTAMSSTPVDASDDV
                     AARAVHLPRPGHADLVGALKYQRTDARDILERASARETTARVAAGSIAKRLLAEFDIS
                     VGSHVVMLGGVEAPLPDQLPEDLNAVADTSPVRVIDPTVQRRMISAIDAAKATGDTLG
                     GVFEVVVRGLPVGLGSHVSWDRRLDGRIAQALMSIQAMKGVEIGMGFESARRPGSQVH
                     DVIETDSNAERAGGYKRSSNRAGGLEGGMTTGAPLIARVAMKPLSTLMRPMQTVNLQT
                     GET"
BASE COUNT          189 a          349 c          285 g          188 t
ORIGIN      
        1 tgtctcgccc gtctgcagat tcaccgtctg catggggcgc atcaatgtcg agagcggctt
       61 catcgcaacc cgcgcgatca acggcgcgcc cgtcgtcatg ccaccttcga gaccaccggc
      121 gcggttcgag gatcgcttgt acccacctgc acgctcagcg ttcgaatctg tttcaattac
      181 gtcatgaact tgcgatcccg gacgccgcgc agattcgaag cccattccta tttctacacc
      241 cttcatcgcc tgaatggaca tgagcgcctg cgcgatgcgt ccatccagcc tgcgatccca
      301 gcttacgtgg cttcccaatc caaccggcag accgcgcaca acaacttcga aaacaccgcc
      361 cagcgtgtcg ccggttgcct ttgctgcatc aatcgcggag atcatgcggc gttgcacagt
      421 cggatcgatc acgcgcaccg gggatgtgtc ggcaactgcg ttcaaatctt ccggcaattg
      481 atccggcagc ggagcttcca caccgccgag cattacgacg tgactgccaa cgctgatatc
      541 gaactccgcg agcaggcgtt tcgcaatgga gccggcggcg acacgcgcgg ttgtttcacg
      601 cgcggatgcc cgctcgagaa tgtcacgcgc gtcggtgcgc tggtatttga gtgcgcctac
      661 gagatcggcg tgaccaggac gcggaagatg aacggcacgc gcggcgacgt cgtcgctcgc
      721 atcaaccggt gttgatgaca tcgcggtctg ccagttttcc caatcgcggt tccagatgac
      781 gagcgctatg ggtgagccga gtgtttcacc cagccgaaca ccggaaagga actcggcgcg
      841 atcctgctcg atcttcatgc ggtccccgcg gccgtacccg ccctggcgcc gccgcagctc
      901 cggatcgacg tcgcgcccga cctccagcgc cagccccgcc ggcacccctt cgatcacgac
      961 gacaagcgcc ggaccgtgcg actcacccgc cgtcgtataa cgcagatgca t
//
LOCUS       DASPLT010000365         2994 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_250428, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000365 DASPLT010000000
VERSION     DASPLT010000365.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 2994)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2994)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2994
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_250428"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2090)
                     /locus_tag="VEA63_03785"
     CDS             complement(<1..2090)
                     /locus_tag="VEA63_03785"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HYD83139.1"
                     /translation="MNPPAPPPDSTSVELARVQEALRVSEAGRRESQTLFEKSFEANP
                     AFMTIGRLADGRLLEVNPAFIRGSGYTREEVIGRSTLELNLWMTTGERDEFVRRMQQD
                     GVVRDFEANFRTKSGEVRALILNADIIQLDGPPCMLTVGVDITERRRRERVQAATYEI
                     SQAVLASGDLGALFKEMHRIVGELVPARNFYIALLSADGAELSFPFFVDDHVTSPPPR
                     KPGNGFTEYVLATGQPLRATAAELAEILAARGFYQRLDHPAAQRLGVPLRIEGRAIGV
                     IALQDYENPNAYSEEDKQLLMFVADQIAAAVHRREAEEALRRAELQYRSIFENALEGL
                     YQTSPDGRFLKANPALAKMCGYATPEELVRSMNDIGHQLYVKPGRRAEFLRLIERRDE
                     VTDFHSQIRRRDGTTLWIGESVRVIRDAQGKVSHFEGVAVDITQQREVARALQAAKEA
                     ADAASRAKSYFLASVSHELRTPLNGILGYTQILRRDPALGDKQRDGVRVIHDSAEHLL
                     ALINDVLDLSKIEAGRIELQPADFDLVEFIAGVERVLAPRARDKGLLLETQVDEDVPR
                     WVRGDEQRLRQVVFNLVANAVTFTQRGGVVLSVKTDGAALRFSVSDSGPGILPEDVGK
                     LFEPFTQVGAPRAGGTGLGLAISRSLVERMGGRLQVESQPGWGSRFWFALTLPVAAPT
                     AGSVRNSGLRRIIG"
     gene            complement(2195..2641)
                     /locus_tag="VEA63_03790"
     CDS             complement(2195..2641)
                     /locus_tag="VEA63_03790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD83140.1"
                     /translation="MKSAVTSLLVTVALSVTILMLAFNVRASQPAADRASDYGLLVER
                     GRYLVDNVGLCADCHTPRNERGEFVRELWLQGSALGFKPLVEMPWAPAAPPIAGLRAM
                     TDQQAITFLTSGVRPDGSRPLPPMPEYRFSEPDAQAVLAYLRTIGK"
BASE COUNT          572 a         1004 c          908 g          510 t
ORIGIN      
        1 tatccgataa tccgacgcaa tcccgaattc ctcaccgaac cggccgtcgg cgccgccacc
       61 ggcagagtta acgcgaacca aaaccggctg ccccaacccg gttgactctc cacttgcaaa
      121 cgcccgccca tccgctccac caagctgcgg ctgatcgcca gccctaatcc cgtcccgccc
      181 gcgcgcggtg ctccgacttg ggtgaacggc tcaaataatt ttcccacatc ctccggcaga
      241 atacccggcc cggaatcgct cacggaaaaa cgcaacgctg cgccatcggt ttttactgag
      301 agcaccacgc cgcctcgctg cgtgaaggtc accgcattcg cgacgagatt gaacaccacc
      361 tgccgcagcc gttgttcgtc gccgcgcacc cagcgcggca cgtcttcgtc gacctgggtt
      421 tcgagcaaca gtcctttgtc gcgcgcgcgc ggcgcgagca cgcgttccac gccggcgata
      481 aactccacca gatcgaaatc cgccggctgc agttcgatcc gcccagcctc gatcttcgag
      541 agatcgagca cgtcgttgat caacgccagc aggtgctcgg ccgaatcgtg gatcacgcgc
      601 acgccatccc gctgcttgtc tccgagcgcc gggtcgcgac ggagaatctg cgtgtatcca
      661 agaattccat tcaacggcgt ccgcagctcg tggctcacgc tcgcgaggaa ataactcttc
      721 gcgcggctcg ccgcgtccgc cgcctctttc gccgcctgca gcgcccgcgc cacctcgcgc
      781 tgttgcgtga tgtccaccgc cacgccttcg aaatggctta ccttgccctg tgcgtcgcga
      841 atcacacgca ccgattcgcc gatccacaac gtcgtcccgt cgcgccgccg gatttgtgaa
      901 tgaaaatccg tcacctcatc acggcgctcg atcagccgga gaaactccgc gcgccggccg
      961 ggcttcacgt agagctgatg cccgatgtcg ttcatgctgc ggaccagctc ctccggcgtt
     1021 gcgtagccgc acatctttgc cagcgccgga ttcgctttga gaaaacggcc gtcgggcgac
     1081 gtctgataca ggccttcgag cgcattctcg aaaatgcttc gatactgcag ctccgcccgc
     1141 cgcaacgcct cctccgcctc gcgccggtgc accgccgccg cgatctggtc cgccacgaac
     1201 atcagcagct gcttgtcctc ctcgctgtaa gcattcggat tctcgtaatc ctgcagcgcg
     1261 atcacgccga tcgcccggcc ctcgattcgc aacggcacgc caagccgctg cgccgccgga
     1321 tggtccaacc gctggtagaa tccgcgcgcc gccaaaatct ccgccagttc cgccgcggtg
     1381 gctcgcaagg gctggcctgt cgcgagcacg tattcggtga agccattgcc cggtttccgc
     1441 ggtggcggcg acgtcacgtg atcgtcgacg aaaaacggaa acgaaagctc cgcgccatcc
     1501 gcgctcaaga gcgcgatgta gaaattccgc gcaggcacca gttcgccgac gatgcggtgc
     1561 atttccttga agagcgcgcc gaggtcgccg ctggcgagca ccgcctgcga aatctcatac
     1621 gtcgccgcct gcacgcgttc gcgacggcgg cgttcggtga tgtcgacgcc caccgtcaac
     1681 atgcagggcg gaccatcgag ctggatgatg tccgcgttga gaatgagcgc gcgcacctcg
     1741 cccgatttgg tgcggaagtt cgcctcgaaa tcgcgcacga cgccgtcctg ctgcatccgc
     1801 cggacaaact cgtcccgttc gccggtggtc atccagagat tgagctccag cgtggaacgt
     1861 ccgatgacct cctcgcgcgt gtaacccgag ccgcgaatga acgccgggtt gacctcgagc
     1921 aaccgtccat cagcgagccg cccgatcgtc atgaacgccg gattcgcctc gaaacttttc
     1981 tcgaagagcg tctgcgattc gcgccggccc gcttcgctca cccgcaacgc ttcctgcacg
     2041 cgcgcgagtt cgacgctggt cgagtcgggc ggcggggcag ggggattcat ttcagccatg
     2101 acgagaggag cgagcctcac ggataaaata gaaccgcagg caggcaacgg cgaacctgcc
     2161 gtcacgctgc ctgcgggttg ggtgcgtgtg ttgcttattt gccgattgtc cgaagataag
     2221 ccaggactgc ttgcgcatcg ggttcggaga aacggtattc cggcatcggc ggcagtggcc
     2281 gcgagccatc ggggcgaacg cccgaggtga gaaaggtgat cgcttgttgg tcggtcatcg
     2341 cgcgcaagcc cgcaatcggc ggtgcggccg gcgcccacgg catctcgacc aagggcttga
     2401 aacccaacgc tgaaccttgg agccaaagtt cgcggacgaa ttcaccgcgc tcgttgcgcg
     2461 gcgtgtggca gtccgcgcac aagccgacgt tgtcgaccag atagcggccg cgctcgacga
     2521 gcagaccgta gtcggaggca cgatcggcgg ccggctggga ggcgcgcacg ttgaaggcta
     2581 acatcaggat cgtcaccgat agggcgacgg tgacgagtag ggaggtgacg gcggatttca
     2641 tgacgaacag ggcgggggag tgtgagagat taaacgaacg acgagacgcg gcgctcacgc
     2701 cacacggacg agcgcgagtg cgacgagcgc cgtcaaagcg aaggaaaaac aaagttgagc
     2761 gatgcggagt gacatgaaga ggaacgctga aaaatgagag cgggcagcgg caggggcagg
     2821 atcacgttca cgcggccagc acacgcaaat gcgcgccgtc gagcatggtg ttggcgagtt
     2881 cgacgtagaa tcgtgtccac gccgctttcg ttgctggcgt gaattccgcg ccgagcgcct
     2941 tttccaaggc ccagagaaac gccacgcccg ccgtggagta gtgctcctcg gacg
//
LOCUS       DASPLT010000366         1394 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_270747, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000366 DASPLT010000000
VERSION     DASPLT010000366.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1394)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1394)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1394
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_270747"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(224..1021)
                     /locus_tag="VEA63_03795"
     CDS             complement(224..1021)
                     /locus_tag="VEA63_03795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007360429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HYD83141.1"
                     /translation="MPEITPPNLDDAGLQVNTLFVRSRNVLLARAAFGDLFVDYYLHL
                     SRQQMRPSSAADAMFKRALAAFVLHCASRPRNELTAWTINFQQPLLNIFLTGDNETGA
                     VAGRVFEEDVKQLPENLFYADVVRAGHPKRRSTVSFHGGDPMAAAEKFYSQSEQRAAR
                     YFQLDEEEFAMVTEHPDCDIGWMQALTAEQVSGLDEKETLVPVETRVYRWHCGCNQER
                     MLEVLAPTMKQDPEGLFAGDEKIEIRCPRCGARYAITREALEAFVAE"
     gene            1146..>1394
                     /locus_tag="VEA63_03800"
     CDS             1146..>1394
                     /locus_tag="VEA63_03800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD83142.1"
                     /translation="MKPQTEEFLYFLLWTADSVMRPTWRNVSDSFESWAWRNGLGRRL
                     ADLEARKILERHPEPDLSRVVRLTQRGKDLALGGRDPVA"
BASE COUNT          290 a          535 c          317 g          252 t
ORIGIN      
        1 ccccaccggc cagccccaca cattctgccg gatcatcagc ccgacgccga tcacccccag
       61 caccgtgccg atgatttcaa atgcggacat gaattgataa aaaagaacca cgaatggaca
      121 cgaacacaca cgaatgatcg agcaaactca cgccgcctac tcagcacctc attgtgtcaa
      181 ttcgtgtcca ttcttggttg aacaacaaac catccgcggt ccctcactcc gccacaaacg
      241 cctccagcgc ctcgcgcgtg atcgcatacc gcgccccaca ccgcggacac ctgatctcga
      301 tcttctcgtc ccccgcgaac agcccctccg gatcctgctt catcgtcggc gccagcacct
      361 ccagcatccg ctcctgattg cagccgcagt gccagcgata cacgcgcgtc tccaccggca
      421 cgagcgtctc tttctcgtcg agcccgctca cctgctccgc ggtcaacgcc tgcatccagc
      481 cgatatcaca gtccggatgc tccgtcacca tcgcgaactc ctcctcgtcc agttgaaaat
      541 accgcgccgc ccgctgctcg ctctgcgaat aaaatttctc cgccgccgcc atcggatcgc
      601 ccccgtgaaa actcaccgtg ctccgccgct tcggatgacc cgcacgcacc acgtccgcat
      661 aaaataaatt ctccggcaac tgcttcacgt cctcctcgaa cacccgcccc gccaccgcgc
      721 ccgtttcgtt gtcccccgtc agaaaaatat tcagtagcgg ctgctggaaa ttaatcgtcc
      781 acgccgtcaa ttcgttccgc ggacgcgacg cgcaatgcag cacgaacgcc gcgagcgccc
      841 gcttgaacat cgcatccgcc gccgacgacg gacgcatctg ctgccggctg aggtgcaaat
      901 agtaatccac aaacaagtcg ccgaacgctg cacgcgcgag cagcacattc cggctccgca
      961 cgaacaacgt gttcacctgg agcccggcat cgtcgagatt cggaggagtg atttccggca
     1021 tgacaaaaaa cgtgccgcca acccgcccaa tgtccacccc tccccccggt tttcccccct
     1081 tgctctcgaa tgtcgcatat gcgacatcca actgcacacg gtctccccac ccgctttcct
     1141 accccatgaa gccgcaaacc gaagaattcc tctatttctt gctctggact gccgactcgg
     1201 tgatgcgccc gacatggcgc aacgtaagcg actccttcga atcgtgggcg tggcgcaacg
     1261 gactcggccg ccgtctcgcc gacttggaag ctcggaaaat tctcgagcgt cacccagagc
     1321 cggacctctc ccgtgtcgtt cgcctcaccc agcgcggaaa agatctcgcg ctcggcggtc
     1381 gcgaccctgt cgcg
//
LOCUS       DASPLT010000367         1421 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_86857, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000367 DASPLT010000000
VERSION     DASPLT010000367.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1421)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1421)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1421
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_86857"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            22..>1421
                     /locus_tag="VEA63_03805"
     CDS             22..>1421
                     /locus_tag="VEA63_03805"
                     /inference="COORDINATES: protein motif:HMM:NF037015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HYD83143.1"
                     /translation="MDIPPFQIQFRLPLLADLGLTGFLRVPFNQFAGGTRKTLYYGVR
                     VTDSNGNVTQTVSSYDIIPDNAPIIDIIAPRPGAYFPEGIAMGITAQVADDVSVDSVD
                     FIVNDATGQTIYTANAPPFAYGYRIPANSGGEVTISASALDSFGNRSSAPAIRINTRE
                     DYPPSISISNPINGQDVREGRNLLIQAAAGDDIAVRSVTFYVDGEPVRIDTTAPFEMS
                     WDVPFGRLGNAIILGASATDTAGRSTFADQIRVNVAPDGTAPTVTFQEPADNSGIIAG
                     SVFRVRATAQDDVSLSSVVFHVNGSATPHVVDLAAPYEFLYRLPANAADAANAAGRWF
                     TFTATAYDAAGNASVPASLTIEAVRDLAPVISSFTPVDQSTIIDGSLLLLAAVADDDV
                     DVVSVEFFVDGKSVGRDHVLPFNQPFQMPLGRAGTTMTFKAVAKDTAGQTTEKVSVVN
                     VSEDEPPLVELDLPLPG"
BASE COUNT          267 a          436 c          440 g          278 t
ORIGIN      
        1 tttgtcggtt cgctgaacct cgtggatatc cccccgtttc aaatccagtt tcgattgccg
       61 ctgctggcgg atcttgggct gacgggattt ctgcgcgtgc cgttcaacca gttcgcgggc
      121 ggaacccgga agacacttta ctatggcgtg cgtgtgaccg attcgaacgg caacgttacg
      181 cagactgttt cgtcctacga tatcatcccg gataacgcgc cgatcatcga catcatcgcg
      241 ccgaggcccg gcgcgtattt tccggagggc atcgcgatgg ggatcaccgc gcaagtcgcc
      301 gacgacgtgt cggtcgacag cgtcgacttc atcgtgaacg acgcgacggg gcagacaatt
      361 tacaccgcga acgccccgcc gttcgcgtat ggttatcgta ttccggcgaa tagcggggga
      421 gaggtcacaa tttcggccag cgcgctcgac tctttcggca accgctcttc ggcgccggcg
      481 atccgtatca acacgcggga agattatccg ccgagtattt ccatttccaa tccgatcaat
      541 ggccaggatg tcagggaggg ccgaaacctg ctcatccaag ctgcagcggg cgacgacatc
      601 gcagtgcgat cggtcacctt ctacgtcgac ggcgagccgg tgcgcatcga tacgacggcg
      661 cccttcgaga tgtcatggga tgttccgttc ggaaggttgg gcaacgcgat cattctgggc
      721 gcatcggcga cggacacggc cgggcggtcg acgttcgcgg atcagattcg cgtcaatgtc
      781 gcgccggacg gaacggcgcc gacagtcacg ttccaagagc cggcggacaa ctcgggcatc
      841 attgccggca gcgtgttccg cgtgcgtgcc acggcgcagg acgatgtctc gctgtcttcc
      901 gtggtcttcc acgtgaacgg aagtgctacc ccgcacgtcg tcgacctcgc ggcaccctat
      961 gaatttctct accggctgcc ggcgaacgca gccgatgcgg ccaatgccgc cgggcgctgg
     1021 tttactttca cggcgacagc ctatgatgcg gcgggaaatg cgtcggtgcc ggccagtctg
     1081 accatcgaag ccgtgcgcga cctggcgcca gtcatctctt ccttcacgcc ggtcgatcaa
     1141 tcgacaatta tagatggaag cctgctgctg ctggccgccg tggcagacga cgacgtcgac
     1201 gtcgtcagcg ttgagttctt cgtcgacggg aaatccgtcg gacgcgatca cgtgctgccg
     1261 tttaaccagc cgttccaaat gccgctcgga cgcgcgggca cgacgatgac gttcaaagcc
     1321 gtcgcgaagg acacggcagg gcaaactacg gaaaaagtaa gcgtcgtgaa cgtgtcagag
     1381 gatgaaccgc cgctcgtcga gctcgatctg ccgctgccgg g
//
LOCUS       DASPLT010000368         1108 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_27095, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000368 DASPLT010000000
VERSION     DASPLT010000368.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1108)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1108)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1108
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_27095"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1108)
                     /locus_tag="VEA63_03810"
     CDS             complement(<1..>1108)
                     /locus_tag="VEA63_03810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HYD83144.1"
                     /translation="DVLDLDLQPRVARNLGYGEENLLMRVEHFMQDYYRTAQSIFRIS
                     KLVESRLALTIGRDAQGAKVSLRDSLLASRFRRSKKLDGFVLRGRDLAAETPDVFKQD
                     PVRLIRVFRHRQQLDCALDFHLAALIRESLPLLTPEYRDSHDASVTFRAILSEAGAVF
                     PTLNLMHELGALSKFIPEFDGLTCLVQHEFYHRYTADVHTLEAIRQLDLIFTEAEPIT
                     LKYREALHETADPSLLYLTLLLHDIGKSKGIKGHAEMGVEIAQPILQRLDVSPEGQEL
                     IAFVIKNHLVMARFWQKRDVDDPKTAAAFAELVGDAEKLRNLYVHTFCDARGTAASLW
                     NGYKDTLHTSLFRATLDHLSLGDAVQANFETKKQM"
BASE COUNT          207 a          284 c          393 g          224 t
ORIGIN      
        1 tcatttgctt cttcgtttcg aaattggctt ggacggcgtc gccgaggctt aggtggtcca
       61 acgtagcacg gaagaggctg gtgtgaagcg tgtctttgta accgttccaa agactggctg
      121 cggtgccgcg ggcgtcgcag aacgtgtgga cgtagagatt ccggagtttt tcggcgtctc
      181 cgacgagttc ggcgaatgca gcggctgtct tgggatcatc gacgtcacgc ttctgccaga
      241 atcgtgccat caccagatga tttttgatca cgaacgcgat cagctcctgg ccttccgggg
      301 aaacatcgag gcgttgcagg atcggctggg caatctcgac gcccatctcg gcgtgacctt
      361 tgatgccttt ggatttgccg atgtcgtgga gcaggagcgt gagataaagc agcgacgggt
      421 cggcggtttc gtgcagcgcc tcgcgatatt tcagcgtgat cggctcggcc tcggtgaaaa
      481 tcaggtcgag ctgtcggatc gcttccaacg tgtgcacatc ggcggtgtag cggtgataaa
      541 attcgtgctg caccaggcaa gtcaggccgt cgaactcggg gataaactta ctgagtgcgc
      601 cgagctcgtg catgaggttg agcgtcggaa acacggcgcc ggcctcggag aggatcgcgc
      661 gaaacgtgac gctcgcgtca tgggaatccc gatactcggg ggtgagcagc ggcagcgatt
      721 cgcggatgag ggcggcgagg tggaaatcga gcgcgcaatc gagttgctgg cggtggcgga
      781 aaacgcggat gaggcgaacg gggtcctgct tgaagacgtc gggcgtctcg gcggcgaggt
      841 cgcggccgcg gagcacgaaa ccgtcgagct tcttgctccg gcggaaacgc gaggcgagca
      901 ggctgtcgcg cagcgagacc ttggcgcctt gggcgtcgcg gccgatggtg agcgcgagcc
      961 ggctttcgac gagtttcgag atgcgaaaga tcgactgtgc ggtgcggtag taatcctgca
     1021 tgaaatgctc gacgcgcatg agcagatttt cctcgccgta accgaggttg cgcgcgacgc
     1081 gcggctgtag gtcgagatcg aggacgtc
//
LOCUS       DASPLT010000369         2492 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_105681, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000369 DASPLT010000000
VERSION     DASPLT010000369.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 2492)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2492)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2492
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_105681"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1379
                     /locus_tag="VEA63_03815"
     CDS             <1..1379
                     /locus_tag="VEA63_03815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HYD83145.1"
                     /translation="PAFYDAKKNAYLDAGVARAIADKIATRGTNFWYDAKPAEILEGI
                     ALPAHWPAPAELVCGRDTLDVWIDSGSSHAAVLKHAQGATQWPADLYLEGSDQHRGWF
                     QSSLWTSVIAFDRAPYRALLTHGFIVDQERNKISKSSTYEKPQTSDAYVAEYGADVIR
                     LWIASQDFRNDIPISKEILSHVGETYRLIRNTFRYQLSNLFDFDHARDAVPVEQMDVL
                     DRWALHQTAALIEQCTVAYENYEFHRVYQLCNQFCSVTLSATYHDILKDRLYTLGTHS
                     PLRRSSQTAIHHIFQALVKLLAPILTFTTDEAWSYATAKTEYSDDSIHLQEWPVAPAT
                     WTNPTIEADFTALLKVRTQVNEAIEPQRAAGKLGKSLDASVTLAGASDDTTFRTLEKH
                     RDFLPELFIVSHVTLAPTMGAALSASIRPSGELGHVRCPRCWRWVPSLESSSPQGVCP
                     RCAEALKP"
     gene            1409..2341
                     /locus_tag="VEA63_03820"
     CDS             1409..2341
                     /locus_tag="VEA63_03820"
                     /inference="COORDINATES: protein motif:HMM:NF013427.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HYD83146.1"
                     /translation="MAKNNKKTAKSKVAKPVLKKKPSAPIKARAAKPAPKALKPAAKP
                     VQKPAAKPTAKPAKPSPTMEKPSKKNALRDSILKRKATTKPIAFSLDEVRAIAKTVTP
                     KAEDEKPQKAAKAAAAKATLALEKPAKPNHIKAASLSDILGFNPKRPRVTNPAEAREV
                     PEKFKRFYKLLLDLRQHLTEGIQLHSEETLKRSSKDDSGDLSSYGQHMADAGTDTFDR
                     DFALSLVSSEQEALTEIDAAIKRIHDGSYGICEITQKPIAKERLLAVPFTRYSAEAQK
                     DLEKNRYRTRTAGGLFGELGEEGGKIMEEDGGDE"
BASE COUNT          553 a          894 c          629 g          416 t
ORIGIN      
        1 ttcctgcttt ctacgacgcg aagaaaaacg cctacctcga cgccggcgtc gctcgcgcga
       61 tcgctgacaa gatcgcgacg cgcggcacca acttctggta tgacgccaag cccgccgaaa
      121 tcctcgaggg catcgcgttg cccgctcact ggcctgcgcc agccgagctc gtttgcggcc
      181 gcgatacgct cgatgtgtgg atcgactccg gttcttcgca cgccgcggtg ctcaagcacg
      241 cacaaggcgc cacgcagtgg cccgccgatc tttatctcga gggcagcgac cagcaccgtg
      301 gctggtttca atcttcgctc tggaccagcg tgatcgcttt cgaccgcgcg ccctatcgcg
      361 cgttgctcac gcacggcttc atcgtcgatc aggagcgcaa caagatctcc aagagttcca
      421 cctacgagaa accgcaaacg agcgatgcct atgtcgcgga gtatggcgcc gacgtcattc
      481 gcctttggat cgcgtcgcag gactttcgca acgacatccc gatctcgaag gaaatcctct
      541 cgcacgtcgg cgaaacctac cggctcatcc ggaacacgtt tcgttatcag ctctccaatc
      601 tcttcgactt cgaccacgcg cgcgacgccg tgcccgtcga acagatggac gtgctcgatc
      661 gctgggcact gcaccaaacc gccgcgctca tcgagcagtg caccgtcgcc tacgagaact
      721 acgagttcca ccgcgtctac cagctctgca accagttctg ctccgtcacg ctttccgcga
      781 cgtatcacga cattctcaag gaccggcttt acacgcttgg cacgcactcg ccgcttcgcc
      841 gttcctcgca gaccgcaatc catcacatct tccaagcgct ggtgaaactc ctcgcgccga
      901 tcctcacgtt caccactgac gaagcgtggt cttacgcgac agcgaaaacc gaatactccg
      961 acgactccat tcacctccaa gaatggccgg tcgcacctgc cacgtggacc aatcccacga
     1021 tcgaagccga cttcaccgcg ctcctgaaag tgcgcacgca ggtcaacgaa gccatcgagc
     1081 cgcagcgcgc cgccggcaag ttgggcaaat cgctcgacgc ctccgtcacc ctcgccggcg
     1141 ccagcgacga caccacgttc cgcacgctgg aaaagcaccg cgattttctc cctgaactct
     1201 tcatcgtttc ccatgtcact ctcgccccaa ccatgggagc cgcgctcagc gcgagcatcc
     1261 gtccgagtgg cgagcttggt cacgtgcgct gcccccgctg ctggcgttgg gtgccatcgc
     1321 tcgaatcgag ttcaccgcaa ggcgtatgtc cgcgttgcgc tgaagccttg aagccatagt
     1381 ttctcccgcc gcttctcccc aataccccat ggccaagaac aacaaaaaaa ccgccaagtc
     1441 caaagtcgcc aaacccgtgc tgaaaaaaaa gccgtctgcg ccgatcaagg cgcgggcggc
     1501 caaaccggcg cccaaggctc tcaagcccgc ggccaaaccg gtgcaaaaac ccgcggcaaa
     1561 gccgactgcc aaaccggcga aaccctctcc caccatggaa aaaccctcca agaaaaacgc
     1621 gctgcgcgac agcatcctga agcgcaaagc caccaccaaa cccatcgcct tcagcctcga
     1681 cgaagtccgc gccatcgcga aaaccgtcac gccgaaagcg gaggacgaaa aaccgcagaa
     1741 ggccgccaag gccgctgccg cgaaggcgac gctcgcgctc gaaaaacccg cgaagcccaa
     1801 tcacatcaag gccgcttcgc tgtctgacat cctcggcttc aatccgaagc gtccacgcgt
     1861 caccaatccc gcggaagcgc gcgaagtccc cgagaaattc aagcgtttct acaaactcct
     1921 cctcgatctt cgccagcatc tcaccgaagg catccagttg cactcggaag agacgctcaa
     1981 gcgctccagc aaggacgact ccggcgatct ctccagctac ggtcagcaca tggccgacgc
     2041 cggcaccgac accttcgatc gcgatttcgc gctgagcctc gtctccagcg aacaggaagc
     2101 gctcaccgaa atcgacgccg cgatcaaacg cattcacgac ggctcctacg gcatctgcga
     2161 aatcacgcag aaaccgatcg ccaaagagcg cctgctcgcc gtgcctttca cgcgctactc
     2221 cgccgaagct cagaaggacc tggaaaagaa tcgctatcgc acccgcacgg ccggcggcct
     2281 cttcggcgag ctcggcgaag aaggcggcaa gatcatggaa gaagacggcg gcgacgagtg
     2341 atggcgctta gagatttctg attctcgatt ttcgattgaa ctcaacggcg acgaactctc
     2401 cgcagcccgc ggcccccgtg ccgcctcaat cgaaattcga gaatcgaaat tcgaaaatcc
     2461 agcgcttcct cgcctatcgc cggctgctgc tg
//
LOCUS       DASPLT010000370         2732 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_27098, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000370 DASPLT010000000
VERSION     DASPLT010000370.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 2732)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2732)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2732
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_27098"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2046)
                     /locus_tag="VEA63_03825"
     CDS             complement(<1..2046)
                     /locus_tag="VEA63_03825"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HYD83147.1"
                     /translation="MNSLNVSANANAAVIEAAYEAWQQNPDSVDPTWRAFFQGFTLGS
                     GGADLTAAPASGVKIIDSVRQAQVARLINAYRAHGHLQAHLDPLGEPPPPHPRLSLAH
                     FGLEESDLGETFTLTNFRGGGQQKLADILDALQRTYSGHIGVEYMHCQDHDAREWLQE
                     KMEAATNQPSFTKKQKVRILRRLHKAELFEKFLHTKYVGQKRFSGEGAETFIAAMDAM
                     IEKCPELGVEEIVMGMAHRGRLNVLVSIMRKPFEILFEQFSENYIPESVGGDGDVKYH
                     LGYEAILDTTSGKKIEVRLAANPSHLEIVDPVVEGKARARQRVRGDADERRRVLPFLI
                     HGDAAFAGQGVVAETLNFSQLPGYRTGGTVHLVINNQIGFTTLPAEARSTRYCTDVAK
                     MIEAPIFHVNGDDAEAVCYVAQLALEFRVKFKRDIVIDMVCYRRHGHNEGDEPAFTQP
                     TMYRKIAAHPQVSAILTEKLVAQGTIAQAEADAIKAEYSAALEANFEKAKSRETAKGP
                     RQSQAIEARKYEGSNGFVQPDYRHSTVNTGVSAETLDRVVTALTKIPGDFKINPKIRR
                     LLENRTQAYKEGGPIDWGFGEALAFGSLLLEGTPVRLSGQDTERGTFSHRHAVLYDSD
                     TRAKYVPLKHLGDDQASFCVYNSLLSEAAVLAFDYGYSMDYPQMLCIWEAQFGDFANG
                     "
     gene            2231..2473
                     /locus_tag="VEA63_03830"
     CDS             2231..2473
                     /locus_tag="VEA63_03830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD83148.1"
                     /translation="MEIEIKTAVSGLLEAIKAADGQRVASEMARLDEMLERGRATLHP
                     QLVHFLQNRSYAKALMFLGGATDIPVGACGGRAGRS"
BASE COUNT          488 a          677 c         1030 g          537 t
ORIGIN      
        1 gccgttggcg aagtcgccga actgcgcctc ccagatgcag agcatctggg gatagtccat
       61 cgagtagccg tagtcgaacg cgagcacggc ggcttcggag aggagcgagt tgtagacgca
      121 gaacgaggct tggtcgtcac cgaggtgttt caggggaacg tatttcgcgc gcgtgtcgga
      181 atcgtagaga accgcgtggc gatggctgaa cgtgccgcgc tccgtatcct gtccgctgag
      241 acgcacgggc gtgccttcga ggagcagcga gccgaaggcg agcgcttcgc cgaagcccca
      301 gtcgatgggt ccgccctcct tgtaagcctg tgtgcggttt tcgagaaggc ggcggatctt
      361 cgggttgatt ttgaaatcgc cgggaatctt cgtgagcgcg gtgacgacgc gatcgagcgt
      421 ttcggcgctg acgccggtgt tgaccgtcga gtggcgatag tcgggttgga cgaagccgtt
      481 tgagccttcg tatttgcgcg cttcgatggc ttgcgactgg cgcgggcctt tcgcggtttc
      541 acgcgatttg gctttttcaa aattggcttc gagcgcggcg gagtattcgg ccttgatcgc
      601 gtcggcctcg gcttgggcga tggtgccttg ggcgacgagt ttttcggtga ggattgcgga
      661 gacctgcggg tgcgcggcga tcttgcgata cattgtgggc tgcgtgaacg cgggctcgtc
      721 gccctcgttg tggccgtgac ggcggtagca aaccatgtcg atgacgatgt cgcgcttgaa
      781 cttcactcga aactcgagcg cgagttgcgc gacgtagcag acagcctcgg cgtcgtcgcc
      841 gtttacgtgg aagatcggcg cctcgatcat cttggcgacg tcggtgcagt aacgcgtgga
      901 gcgcgcctcg gcggggagcg tggtgaagcc gatctgatta ttgatgacga ggtgaacggt
      961 gccgccggtg cggtagccgg ggagttgcga gaagttgagc gtttccgcga cgacgccctg
     1021 gccggcgaac gcggcgtcgc cgtggatcag gaaaggcagg acgcggcggc gttcgtcggc
     1081 gtcgccgcgg acgcgctggc gggcgcgggc tttgccttcg acgacgggat cgacgatttc
     1141 gaggtgagac ggattagccg cgaggcggac ttcgattttc ttgccggagg tggtgtcgag
     1201 aatcgcttcg taaccgaggt ggtatttcac gtcgccgtcg ccaccgacgg attcgggaat
     1261 gtagttttcg gaaaactgtt cgaagaggat ttcgaacggt ttgcgcatga tcgacacgag
     1321 gacgttgagg cggccgcggt gggccatgcc catgacgatt tcttcgacgc cgagttcagg
     1381 gcatttctcg atcatcgcgt ccatggcggc gatgaacgtc tcggcgcctt cgccagagaa
     1441 gcgtttctgg ccgacgtatt tcgtgtgaag aaatttttcg aagagctcgg ccttgtggag
     1501 gcggcggagg atgcggacct tttgcttctt ggtgaacgac ggctggttgg tggcagcctc
     1561 catcttttcc tggagccact cgcgcgcgtc gtggtcctgg cagtgcatgt attcgacgcc
     1621 gatgtgcccg ctgtaggtgc gttggagagc gtcgaggatg tcggcgagtt tctgctgacc
     1681 gccgccgcgg aaattcgtga gcgtgaaagt ctcgccgagg tcggattctt cgaggccgaa
     1741 atgagccagc gagaggcgcg ggtgaggcgg cggcggctcg ccgaggggat cgaggtgcgc
     1801 ctggaggtgg ccgtgggcgc ggtaggcgtt gatgagccgg gcgacctggg cctggcgaac
     1861 gctatcgatg attttgaccc cggacgcggg ggcggcggtg agatccgcgc cgccggaacc
     1921 gagagtgaag ccttggaaaa aggcgcgcca cgtcgggtcg acggagtcgg gattctgttg
     1981 ccacgcttcg taagccgcct cgatcacggc agcattggcg ttggcggaga cattcagcga
     2041 attcatggag gagaggaaac gcgcctcggg tgcgggaact ggcgacggaa atagaggcgg
     2101 cgcgtcatga ggagaaagtt ggtgaaacca acatcacttg tgcggagaga caagcttggg
     2161 gtggacagag gccgggattg caatcgatct aaacgggcgt aacagcggga taagttttct
     2221 cattttgaac atggaaatcg aaatcaagac ggcggtgagc ggtttgctgg aggcgatcaa
     2281 ggcggcggac ggccagcggg ttgcgtctga gatggcgcgg ctggacgaga tgctcgagcg
     2341 cggtcgggcg acgctgcacc cccaactggt gcattttttg cagaaccgga gttacgcgaa
     2401 ggcgctgatg tttttgggtg gcgcgacgga tattccggtg ggggcgtgcg gaggacgggc
     2461 agggcgaagc tgagggcgag gattgagacc tgaaagctga gacctgaaac ctgggggctg
     2521 agacctgggc cgagatttga aaaactaaac ggggacgacg cgacgatggc gggtgaaaag
     2581 aaaatttcga cggacggcgg cggggcgctc ggacaaaatc cgtttgcggc gttgagcgcg
     2641 gcggggttgc cggtggcgcc gaagagcgtg atcgatcgcg cggcggcgga ggcgaaagcc
     2701 gcggttgagc ggaaaggcgg ggcggagacc cg
//
LOCUS       DASPLT010000371         1626 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_27148, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000371 DASPLT010000000
VERSION     DASPLT010000371.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1626)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1626)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1626
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_27148"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1030
                     /locus_tag="VEA63_03835"
     CDS             <1..1030
                     /locus_tag="VEA63_03835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD83149.1"
                     /translation="RGRRAIAVAWIAAGFAIVTGPCIARNVAVSGSPTALAVQNVALK
                     AGDPTAEPATQRNTLSAALPTIDLRKLGNKTLTSLEENLESRVWSGGAMWLTAFFAAG
                     WLYAFRSDVANRLRWVFTASLGVLLVVQSAFNSGESERLAAGWLAPLIIVFGVGFFFV
                     LLGSNARLGQWPRLCAAALLLVQALPLAHDVLEPRRLHFQYPPYFPSLFMGMRQELER
                     REGANRFGVMADVPAGVAWYGRQRVWSQPVKLRDFYAITLEQPMGELLLTPRTLDRPF
                     FSELAARPVEPDSLGRATNRFGEWGTIYAGLFTRVFPAEFPLRAPQKLADNLYVMLNP
                     ALPPPGGK"
     gene            1082..>1626
                     /locus_tag="VEA63_03840"
     CDS             1082..>1626
                     /locus_tag="VEA63_03840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD83150.1"
                     /translation="MNFARAFVAVGLMAALPAWAVYAPLPDQDQNKEWTVSLRAGVSH
                     DSNIFGAASGEISSMVYGFSPKVSYEGSLSDQTFAVFGYTLTLDHIVDRPGDKTLDSH
                     DLAARLAHAFSPTTNIDFSDYYQIAKNPESLLAGLPINTDQSFKRNEFNARFTTSPRP
                     KFGTLLKFRSVNYDYDNAALAT"
BASE COUNT          251 a          455 c          577 g          343 t
ORIGIN      
        1 gaggggacga cgcgcgatcg cggtggcgtg gattgcggcg gggtttgcca tcgtgacggg
       61 accgtgtatc gcgcggaacg tcgcggtcag cggatcgccg acggcgctgg cggtgcagaa
      121 tgtggcgttg aaagcgggcg atcccaccgc ggagccggcg acgcagcgca acacgctttc
      181 ggcggcgctg ccaacgatcg atctgcgcaa gctcggcaac aagacgctca cgagcctcga
      241 ggagaatctc gagtcgcgcg tgtggtcggg cggggcgatg tggttgacgg cgtttttcgc
      301 ggcgggctgg ttgtatgcgt ttcggtcgga cgtggcgaac cggctgcgtt gggtatttac
      361 cgcttcgctc ggcgttttgt tggtggtgca gtcggccttc aattcgggtg aaagcgagcg
      421 actggcggcg ggctggctcg cgccgctgat catcgttttt ggcgtcggat ttttctttgt
      481 gctgctcggt agcaatgcgc ggctcggcca gtggccgcgg ttgtgcgcgg cggcgctgct
      541 gctcgtgcag gcgttgccgc tggcgcacga tgtgctcgag ccgcggcggc tgcattttca
      601 gtatccgccg tattttccga gtttgttcat gggcatgcgg caggaattgg agcgacgcga
      661 aggagcgaat cggtttggcg tgatggcgga tgtgcccgcg ggggtggcgt ggtatggccg
      721 gcagcgcgtg tggtcgcagc cggtgaagct ccgcgatttt tatgcgatta cgctcgagca
      781 gccgatgggc gagttgttgc tgacgccgcg cacgctcgac cggccgtttt tttccgagct
      841 ggcggcgcga ccggtggagc cggattcgct cgggcgcgcg acaaatcgtt tcggtgaatg
      901 gggcacgatt tacgcggggc ttttcacgcg ggtctttccg gcggaatttc cgctgcgcgc
      961 gccgcagaag ctcgcggata atctctacgt gatgctcaat ccggcgctgc cgccgccggg
     1021 gggaaagtaa ttgcttggtg gtgaacgcgg tgtctagcgt ccgcggtcct gtttccacct
     1081 gatgaatttc gcgcgtgcct ttgttgcagt cggattgatg gcggcattgc cggcgtgggc
     1141 ggtgtatgca cccctgccgg accaggacca gaacaaggag tggaccgtgt cgctgcgcgc
     1201 gggcgtgtcg cacgacagca atattttcgg cgcggcctcg ggcgagattt ccagcatggt
     1261 ctacggcttc tcgccgaagg tttcttacga gggctcgttg agcgatcaaa cgtttgcggt
     1321 gttcggctac acgttgacgc tcgaccacat cgtcgaccgg cccggcgaca agacgctcga
     1381 cagccatgat ctcgcggcgc ggctcgcgca tgcgttcagc ccgacgacga acatcgattt
     1441 cagcgactat tatcagatcg cgaaaaaccc ggagtcgttg ctcgcgggcc tgccgatcaa
     1501 cactgaccaa tcgttcaaga gaaacgagtt caacgcgcgt ttcaccacga gcccgcggcc
     1561 gaagttcggc acgctgctga agtttcgcag cgtgaattac gactacgaca acgcggcgct
     1621 ggccac
//
LOCUS       DASPLT010000372         1151 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_250507, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000372 DASPLT010000000
VERSION     DASPLT010000372.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1151)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1151)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1151
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_250507"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..298
                     /locus_tag="VEA63_03845"
     CDS             <1..298
                     /locus_tag="VEA63_03845"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HYD83151.1"
                     /translation="GNEALSADAFGVPSPPSTGADLSPRLLLVPLLAFDRAGGRLGQG
                     GGHYDRIISLYRAHGAIAVGLAFAEQEMDAVPTGPHDAHLDWVITPKEAIRCAR"
     gene            complement(764..>1151)
                     /locus_tag="VEA63_03850"
     CDS             complement(764..>1151)
                     /locus_tag="VEA63_03850"
                     /inference="COORDINATES: protein motif:HMM:NF016246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HYD83152.1"
                     /translation="IGYEAIAELGGLRGKQRQIEPSPNGLWRVKSFRNYADYALGEKF
                     AQGFARLRQVGHERRCAIMCAEAVWWRCHRRLIADYLLAAGEEVFHIMGPGHVDAAKL
                     TPGAVVQRSDLVMYPAPADLISVVAE"
BASE COUNT          196 a          380 c          364 g          211 t
ORIGIN      
        1 gggcaatgaa gccttgagcg ccgacgcttt cggcgtgccg tcgccgccct cgaccggcgc
       61 cgatctcagc ccacgcctcc tgctggtgcc gctgctcgcg ttcgaccgcg ccggcggccg
      121 tttgggccaa ggcggcggcc actatgaccg catcatttcg ctctaccgcg cccatggcgc
      181 gattgccgta ggtttagctt tcgcggagca ggaaatggat gccgtgccca ccggcccgca
      241 cgacgcgcat ctcgactggg tcatcacgcc gaaagaagcg atccgctgcg cgcgctgaag
      301 ggctgcgcgg gagaaggtga ggcgtagcat ggactctgcg accgccaggc ttatgatgtt
      361 cgaatgctga gattttgcgt tctcatagtt tcagttgcct tcgtcgcggc agctggggcc
      421 gagacgaacg ctggcgtcgc gcttgcgcct ccaagtgcgg atgcgcttga ggccgccgag
      481 cttctcgact caccgccctg ggtcgagcag ccaaacgaat acgattattg gaatctgttt
      541 ccgcccgaac ggccaacgac aagcaatatt tcgcgtggcc gcggtgggcg gatgaatgag
      601 agtgtcgagt tcgactgcct ggtgcaagcg gatgggcgac tggcttgcgc agtggaggcg
      661 cgcgagtcgc ttgtgacgcc ggcactgacg ttggctacta agttccgtgt ggcgtctcac
      721 acacaggatg gcgagccgac cgccggacgc cgcattcgcg aaatcattcg gctacgacgc
      781 tgattaagtc cgcaggcgcg ggatacataa cgaggtcgct tcgctgcacc accgccccag
      841 gcgtcagttt cgccgcgtcc acgtgccccg gccccatgat gtggaacacc tcctcgccgg
      901 cggcgagcag ataatcggcg ataaggcggc ggtggcaccg ccaccacacg gcctctgcgc
      961 acatgatcgc gcaccgccgc tcatggccga cttgccgcaa tctggcgaac ccctgcgcga
     1021 acttctcgcc gagcgcatag tcggcgtaat tccgaaagct cttcacccgc cacagcccgt
     1081 tcggcgaagg ctcgatctgc cgctgtttgc cgcgtaggcc gccaagctcg gcgatcgcct
     1141 cgtaaccgat c
//
LOCUS       DASPLT010000373         1179 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_209733, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000373 DASPLT010000000
VERSION     DASPLT010000373.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1179)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1179)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1179
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_209733"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1179)
                     /locus_tag="VEA63_03855"
     CDS             complement(<1..>1179)
                     /locus_tag="VEA63_03855"
                     /inference="COORDINATES: protein motif:HMM:NF033679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNRLRE domain-containing protein"
                     /protein_id="HYD83153.1"
                     /translation="AFLAAFVAPVTAQTVTLQPTADAHVLSGPFLENLNLGKSPLLEV
                     NHSSLSLAWFEREVYLKFDLSNVTSVRTAKLRLYGVSLGSEIVTTDLLASTDVAWTET
                     GIKWTNKPATGPVLWATTALQDQPGWHEWDISQYLMGEKTMGRNIVTLVLKNSVPTGL
                     GLNIFNSREHFANKPELVIEQAFPWTYYEAETGAWSATAALETGALWGDLAFEARGKQ
                     AVTLDAAGEYLEWTNVKAATHAIIRYSIPDQASGTLALYVNGVKAADLPLSSVMMRET
                     KTGVAPPNGGIVKLYDDVLAAIPGGIPAGATVRVQKDVEDGVPMTVDFLEVETAPAPL
                     TKPDETWVEVMQNTGNDRAAFNAAISTANAGSKKVWIPAGNYVIENAGGDGGMNVPAG
                     "
BASE COUNT          196 a          348 c          388 g          247 t
ORIGIN      
        1 attccagcgg gcacgttcat gccgccgtcg ccgccggcgt tctcgatcac gtaattgccg
       61 gcgggaatcc acaccttctt gctgccggcg ttcgcggtgc tgatcgccgc gttgaacgct
      121 gcgcgatcgt tgccggtgtt ctgcatgacc tcgacccagg tttcgtcagg cttcgtcagc
      181 ggcgcgggtg cggtttcgac ttcgaggaag tcgaccgtca ttggcacgcc gtcttcaaca
      241 tccttttgga cgcgcactgt cgcgcccgcg ggaatgccgc cgggaatcgc ggcgagcacg
      301 tcgtcgtaaa gtttgacgat gccgccgttg ggcggcgcaa cgccggtttt tgtttcgcgc
      361 atcatcacgg acgaaagcgg caggtcggcg gctttcacgc cgttcacgta gagcgcgagc
      421 gtgcctgaag cctgatcggg aatgctgtag cgaatgatcg catgcgtcgc cgccttcacg
      481 ttggtccatt cgaggtattc gccggcagcg tcgagcgtca cggcttgttt gccgcgtgct
      541 tcgaaagcga gatcgcccca gagcgcgccg gtttcgagcg cggccgtcgc actccacgcg
      601 ccggtctccg cctcgtaata cgtccagggg aacgcctgct cgatcacgag ttcgggcttg
      661 ttggcgaagt gctcgcgtga attgaaaatg ttgagcccga gtcccgtcgg cacggagttc
      721 ttgagcacga gcgtgacgat gttacgtccc atggtttttt cacccatgag gtattggctg
      781 atatcccact catgccagcc gggctgatct tgcagtgcgg tcgtcgccca gagcaccggc
      841 ccggtggcgg gtttgttggt ccatttgatt ccggtctccg tccaggcgac atcggtcgac
      901 gcgagcagat ccgtggtgac gatttcggac ccgagcgaca cgccgtagag acggagcttc
      961 gcggtgcgca cgctggtgac gttcgacaaa tcgaacttca agtagacctc gcgctcgaac
     1021 caagccaacg agagcgagct gtggttgacc tcgagcaagg gcgatttgcc gagattgagg
     1081 ttttccagga atggtccgga gaggacatgg gcgtcggcgg tcggttggag cgtgacggtc
     1141 tgcgccgtga cgggcgcgac gaacgccgca agaaaagcg
//
LOCUS       DASPLT010000374         1222 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_229179, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000374 DASPLT010000000
VERSION     DASPLT010000374.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1222)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1222)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1222
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_229179"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..202
                     /locus_tag="VEA63_03860"
     CDS             <1..202
                     /locus_tag="VEA63_03860"
                     /inference="COORDINATES: protein motif:HMM:NF025563.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="RICIN domain-containing protein"
                     /protein_id="HYD83154.1"
                     /translation="VGQWGNGSSNNQRWTISDVGGGYFKLRNVATGLCLDVGASPWAN
                     GDPVEQWPDGSSQNMHWQFVLP"
     gene            261..>1222
                     /locus_tag="VEA63_03865"
     CDS             261..>1222
                     /locus_tag="VEA63_03865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD83155.1"
                     /translation="MSLFRIAFVCVAFVVFAQRITAADAIVEVSTLAEFARVAAGDRQ
                     QVKLRPGVYRMADYLTEDVLAQIHANVDRTQKRPPVPMFVFHGSDNRFDLRDVVIEID
                     TALYAKLPQGGYTRCLIVEGARNRFSGLTIRNTGENRGSGGNILSIAGAENTLEDFVL
                     QVHGSFPFGYGDLLGKGGPNLVPLQKQSGIQILGTRTTLRRCKVFSRALGHCFYIQAG
                     DDIRLEDCYAEGVMRATNDMLRDRSGPAFELGFPSVYENRDGRYLITPGYVKSLVEDG
                     FRTYGGAGNVTLVNCLARHTRAGFEIGAKDDAPRKTVIENCTAMG"
BASE COUNT          220 a          363 c          397 g          242 t
ORIGIN      
        1 cgtcggccag tggggcaacg gttccagcaa caaccagcgc tggacaatca gcgacgtggg
       61 cggcggttat tttaaacttc gcaatgtcgc gaccggtctc tgtctcgacg tcggcgcgag
      121 tccgtgggcc aacggcgatc cggttgagca atggcccgac ggcagcagtc agaacatgca
      181 ttggcagttc gtgttgccgt gagctcgtcc gttcgcggtc cacagacgct accagtggta
      241 gcgcagctcg cgtggtattc atgagtctgt ttcgcatcgc gttcgtctgc gtcgcatttg
      301 ttgtgttcgc acagcgcatc actgcggcgg acgccatcgt cgaggtgtcc acgcttgcgg
      361 aattcgcgcg cgtcgcggcg ggagatcgcc agcaagtgaa gctgcggccg ggcgtttatc
      421 ggatggcgga ctacctcacc gaggatgtcc tcgcgcagat ccacgcgaat gtggatcgca
      481 cgcagaaacg tccgcccgtg ccgatgttcg ttttccacgg aagcgacaac cgcttcgatc
      541 tgcgcgacgt ggtaatcgag atcgacaccg ccctttatgc gaagttgccg cagggcggct
      601 acacgcgatg tctcatcgtc gaaggggcgc gcaatcgctt tagcgggctg acgattcgca
      661 acactggcga aaaccgcggc agcggcggca atatcctctc catcgccggc gcggaaaaca
      721 cgctcgagga cttcgtgcta caggtgcacg gctcgtttcc gttcggttac ggcgatctcc
      781 tcggcaaggg tgggccgaac ctcgtcccgc tgcagaaaca aagcggtatt cagattctgg
      841 gcacacgcac gacgttgcgc cgctgcaagg tcttcagccg cgcgctcggg cattgtttct
      901 acattcaggc gggcgatgac atccgtttgg aggactgcta cgcggagggc gtgatgcgtg
      961 cgacgaacga catgttgcgc gacaggtccg ggccggcgtt cgagcttggg tttccgtccg
     1021 tttacgaaaa tcgcgacggc cgctacttga ttacgcctgg ttacgtgaag agtctcgtcg
     1081 aggacggttt tcgcacctac ggcggggccg gcaacgtgac gctcgtcaac tgtctcgcgc
     1141 gccacacgcg cgccggcttc gagattggcg cgaaggacga tgcgccgcgg aagaccgtga
     1201 tcgagaactg caccgcgatg gg
//
LOCUS       DASPLT010000375         2159 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_250513, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000375 DASPLT010000000
VERSION     DASPLT010000375.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 2159)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2159)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2159
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_250513"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            303..1946
                     /locus_tag="VEA63_03870"
     CDS             303..1946
                     /locus_tag="VEA63_03870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007363423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HYD83156.1"
                     /translation="MSPAQEIARRRTFAIISHPDAGKTTLTEKFLLYGNAIHLAGTVT
                     ARKNQRATASDWMELEKQRGISISSTVLQFDYTGYAVNLLDTPGHKDFSEDTYRVLTA
                     VDAALMVIDAAKGVEAQTRKLFEVCRRRGVPIFTFMNKCDRPTRNALELLDELEQVLG
                     LQSSPVVWPLGNGPSFRGVFDRRTRQVHLFERTPGGKYQAPVEVTSLDDPLVREKLDD
                     YTYEQVKEQLEMLDGAGHPFDLAAVRAGQQTPVYFGSAVNNFGIQLLLDGFLKDSVPP
                     APRRCAPISNFKSQIPNSPAGVIPVTHPKFSAFAFKIQANMDPKHRDRIAFVRICSGK
                     FERDMTVIHQRTGKTVRLSSSHKLFGQERETVDEAWPGDVIGLVGHSDFHIGDTLTED
                     KSIVFDEIPRFPPEVFTFISNPNPSDAKKFRAGLDQLLQEGVVQSITLRAGMTTATLL
                     AAVGVLQFEVVQFRLESEYNAASRLDPAPWTLMKWMSPADPSAKLPDTAQLVVASGVA
                     FGTDKLGQPVALFPNEWTMRYFVEKNPGIQLHDLPLEQT"
BASE COUNT          429 a          846 c          503 g          381 t
ORIGIN      
        1 gccggatcgc gtccgcattc gccacgacac tcgtcgcctt caatgccacc tcgtgctttg
       61 ctttcagctt cgaactgctg acggcataaa ctgtcaccgc cgccatcgcc agcagcgcca
      121 ccaaaccgcc cagcacataa ccaagaactt tcagaagttt aagcatggtg aacagtttcg
      181 agcagctccc tcgcgccgag gcaaattttt cctcttccct cgccccgggc atcccggttt
      241 gctgaccaac tccctccgac tctcccgtcc actgtctcac cgtctaccgt ccactgtccg
      301 ccgtgtctcc cgcccaagaa atcgcccgcc gccgcacctt cgcgatcatc tcgcaccccg
      361 acgcgggcaa aaccacgctc accgagaagt ttctcctcta cggcaacgcc atccatctcg
      421 ccggcaccgt caccgcccgc aagaaccagc gcgccaccgc ctccgattgg atggagctcg
      481 agaaacagcg cggcatctcg atcagctcga ccgttctcca gttcgattac accggctacg
      541 ccgtcaacct cctcgacacg cccggtcaca aggacttctc cgaagacacc taccgcgtcc
      601 tcaccgccgt cgacgcggca ctcatggtga tcgacgccgc gaaaggcgtc gaagcgcaga
      661 cccgcaagct cttcgaggtc tgccgtcggc gcggcgtgcc gatcttcacg ttcatgaaca
      721 agtgcgaccg ccccacacgc aacgcactcg aactcctcga cgaactcgag caagtcctcg
      781 gcctccagtc ctcgccggtc gtctggccgc tcggtaacgg cccttctttc cgcggtgtat
      841 tcgatcggcg gacacgtcaa gttcatctct tcgaacgcac gcccggcggc aaataccaag
      901 cgcccgtcga agtcacttcg ctcgacgatc cactcgtccg cgaaaaactc gacgactaca
      961 cctacgagca agtgaaggaa cagctcgaga tgctcgacgg tgccggccat cccttcgatc
     1021 tcgccgccgt ccgcgccggc cagcaaacgc ccgtctactt cggcagcgcc gtcaacaact
     1081 tcggcatcca actcctcctc gacggcttcc taaaagactc cgtcccgccc gcgccgcgac
     1141 gctgcgcgcc gatctcaaat ttcaaatctc aaattccaaa ttcgccggcc ggcgtcatcc
     1201 ccgtcacgca tccgaaattc tccgccttcg ccttcaagat tcaggcgaac atggacccga
     1261 aacatcgcga tcgcatcgcc ttcgtccgca tctgctccgg caaattcgaa cgcgacatga
     1321 cggtcattca tcaacgcacc gggaaaaccg tccgcctttc ttcttcgcac aaactcttcg
     1381 gccaggaacg cgaaaccgtc gacgaagcct ggcccggcga cgtcatcggt ctcgtcggcc
     1441 acagcgattt ccacatcggc gacacgctga cggaggacaa atccatcgtc ttcgacgaaa
     1501 tcccgcgctt cccgccagaa gtgttcacgt tcatctccaa ccctaaccct tccgacgcga
     1561 aaaaattccg cgccggcctc gaccaactcc tccaggaggg cgtcgtccaa tcgatcacgc
     1621 ttcgcgcggg catgaccacc gccacgctcc tcgccgccgt cggcgtgctc cagttcgaag
     1681 tcgttcaatt ccgactcgag agcgaataca acgccgcctc ccgtctcgat ccagcgccgt
     1741 ggacgctgat gaagtggatg tcgcccgccg acccgtccgc gaaacttccc gacaccgcgc
     1801 aactcgtcgt cgccagcggc gtcgccttcg gcaccgacaa actcggccag cccgtcgcgc
     1861 tctttccgaa cgagtggacg atgcgctact tcgttgaaaa aaatcccggc atccagctcc
     1921 acgaccttcc cctcgagcaa acctaaccac aacgtagcgc gggcaacctg cccgccgctc
     1981 cccattgtag ggcgtggtct cccgaccccg ccgccgctcg ccgaacgtgc cgccgaccgc
     2041 ccccattcgc ccccgcttct tccccacgcc cgccacattt cgcacgtggc tcgagcaaca
     2101 ccacagcacc gccaacgagc tgtgggtcgg cttctacaaa aaatcctccg gccgcggcg
//
LOCUS       DASPLT010000376         1027 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_148006, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000376 DASPLT010000000
VERSION     DASPLT010000376.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1027)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1027)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1027
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_148006"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..396
                     /locus_tag="VEA63_03875"
     CDS             <1..396
                     /locus_tag="VEA63_03875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HYD83157.1"
                     /translation="TDQAWLYVADYRSHWVYSYQIQPDGTLAHKQRFCWLHTPDTDDE
                     AFTDGLKVDREGRLYVTTRLGIQVCDTSGRVQAIIPTPNGKITNLAFVGEHLDTIFAT
                     CNEQVYRRKVKVRGVNAWAPPFKPEVAAR"
     gene            complement(497..>1027)
                     /locus_tag="VEA63_03880"
     CDS             complement(497..>1027)
                     /locus_tag="VEA63_03880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009510816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HYD83158.1"
                     /translation="IRMVVDALDLCYTNAHVDAFAIISGDSDFSPLVSKLRENAKTVI
                     GLGVKNSTSDLFISNCDEFIYYDDLVRAEAPKTRSRAAASKPKAAGEPPKGPDPSKAI
                     DLLVTTVRALTDERGADESIWSSMVKQAIKRRNPGFNERAYGFSSFTDLLREAEKRHL
                     LELEEKSGNCQVRIAE"
BASE COUNT          201 a          264 c          358 g          204 t
ORIGIN      
        1 acggaccaag cgtggctcta cgtcgcggac tatcgatcgc actgggttta cagctatcaa
       61 atccagcctg acggcacgct ggcgcacaag cagcgctttt gctggctgca tacgccggac
      121 accgatgacg aggcgttcac cgacggtctc aaggtcgacc gcgaaggccg gctctatgtg
      181 acgacgcggc tcggcatcca agtttgtgat acgtccggac gcgtgcaggc gatcatcccg
      241 acgccgaatg gaaaaatcac gaacctggcg ttcgtcggcg agcacctcga cacgattttc
      301 gcgacctgca acgaacaggt gtatcgacgc aaagtgaagg tgcgcggggt gaacgcgtgg
      361 gcgccgccgt ttaaaccgga agtggcggcg cggtgaacca tcttccggat atcgaaactg
      421 cgcggcgagg ggaggaagcg gagtttgtca tacaatagat gacaaatgcg cggtggagga
      481 acggagagga aggcggttat tcggcgatcc gcacctggca gtttccggat ttttcctcga
      541 gttcgaggag atggcgtttt tcggcttcgc gcagcaggtc ggtgaacgag ctgaaaccgt
      601 aggcgcgttc gttgaagccc gggtttcggc gtttgatggc ctgtttcacc atggaggacc
      661 agatgctttc gtcggcgccg cgttcgtcgg tgagcgcgcg cacggtggtg acgagcaggt
      721 cgatcgcctt ggaaggatcg gggcctttcg gcggctcgcc ggccgctttg ggtttggagg
      781 cggcggcacg tgaacgggtt ttcggcgctt cggcgcgaac gagatcgtcg tagtagatga
      841 actcgtcgca attcgagatg aagagatcgg acgtggagtt tttcacgccg aggccgatga
      901 cggtcttggc gttttcgcga agcttgctga cgagcggcga gaaatcggag tcgccgctga
      961 tgatggcgaa ggcgtcgacg tgcgcgttgg tgtaacagag atcgagcgcg tcgacgacca
     1021 tgcggat
//
LOCUS       DASPLT010000377         2876 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_250527, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000377 DASPLT010000000
VERSION     DASPLT010000377.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 2876)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2876)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2876
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_250527"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2876
                     /locus_tag="VEA63_03885"
     CDS             <1..>2876
                     /locus_tag="VEA63_03885"
                     /inference="COORDINATES: protein motif:HMM:NF024603.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HYD83159.1"
                     /translation="GTIGADGKFTATVPLVRNATNHLHVTVAGSNGLSSSPALIEVVQ
                     DSVAPTIDHVVPIVSASGVPQESAVQIVFSEAIQATDIAGVQLQSGSNGIAMTRSLSS
                     DAKTLTLTPTAPLAESSTVRVTVPSSVTDLAGNPLASGFTYTFRTVDLTTPATPIVDA
                     APVRTASLIVTISGIAEPFSTISVSGAAGALPAGADAQGRFVIPVTLHPAAANSLQVT
                     ATDADGNTSPAYTLTITHDSQAFTLLSSIPAAAATGIALDSVIALTFSRAIDPASLAG
                     IALVGQAIVPATITVEEDVVSLVPTGPLAAGIRYEIVVPASVADLFGNRLDVTRRIAF
                     TTQGSNELAAPVIYSAGPKGATNQLVATLSGYSSPGTRVLVSGGGAPFTFPASGAIDA
                     TGLFTFEVPLQLNAQNSIVLRASDSEGRLSNPVTALDVRQDATPPDVVATLPTAGAIN
                     VDQHAPLFVEFSEAIQPGPLAASIPAIRLFDAQNALVTGSWILSADSRSATFYATRSL
                     APEANFKLLVGTVVRDLAGNALVAPLEVAFTTAAAGAVDRPATPVLDPLVSTRTTESA
                     ITLRGSAMPGAQLRVFGGENGAAAAVGDDGRFTVVVPLVLNTANPLAIVAEVGGAVSN
                     PAAITIVQVEHAAAVRILSPQAGVTYNNRSLTVAGIIDDPEAVSAVNVGGIAASIVGR
                     YFFAQVVLDETAGAKSVTAVATLKDGSTLETTVAFSLLVEAAGVDTQPPIPRFLFPEE
                     GDVLNGDVVETLLTVEEGVQLTSVEIDRVVAHQVVGNIFFIHARLPQQGLNAISVGAT
                     DAAGHIGTATVNVDVDSIGFATAPTVASLPSLTNNRVITLTGTAEPGSTIVVLNGLVP
                     VRVTVPASGAYVVSVPLNPNAANHLQVVATDAAGNLSPVTTVDIVHDDTPPSIVATSP
                     SSGQTGVPQNATIEVTFSEPLNPASTVSETAVVVRSAGGQAIQR"
BASE COUNT          491 a          981 c          854 g          550 t
ORIGIN      
        1 ccgggaccat cggcgccgac ggcaagttca ctgccaccgt cccgctcgtc cgaaacgcca
       61 ccaaccacct ccatgtcacc gtcgccggct cgaatggtct gtcttcttct cccgcgctca
      121 tcgaagtggt tcaggattct gtcgctccga cgatcgatca cgttgtgccg atagtatcgg
      181 cttcaggcgt gccacaggag agcgcggtcc agatcgtatt cagcgaagcc atacaggcta
      241 cggatattgc cggcgtgcaa cttcagtcgg gctccaatgg gattgccatg acgcgctcgc
      301 tgtcgtccga cgcgaagacc cttacactta ccccgaccgc gccgctcgcc gaatcgagca
      361 ccgtgagggt gaccgtgccg tcatcggtga ctgacctcgc cggcaatccc ttggcgagcg
      421 gcttcaccta cacgttcagg acggtcgatc taacgacccc cgcgacgcca attgtcgacg
      481 cggcgcccgt gcgaaccgcc tcgctcatcg tgacgatctc aggcatcgcc gaaccgttca
      541 gcaccatctc ggtttcgggc gctgccggag cgctcccggc cggcgcggac gcgcaaggtc
      601 gtttcgtcat tccagtcacg ctgcatcccg ccgccgcgaa ttccctgcaa gtcacggcga
      661 ccgacgcgga cggcaacaca tcgcccgcgt atacgctcac gatcacacac gacagtcagg
      721 cgttcactct gctgtcgtct attccagccg cggccgcaac gggcatcgcc ctggactcgg
      781 tcatcgcgct cacgtttagc cgcgcgatcg atccggcctc gctcgccggt atcgcgctcg
      841 tcggccaagc aatcgttccg gctaccatca ccgtggaaga agacgtggtt tcgctggtgc
      901 caacaggccc gctcgcagcg ggtatccgct atgaaatcgt ggttccagcg tcggtcgctg
      961 atctctttgg caatcggctc gacgtcactc gtcgcatcgc gtttacgacg cagggctcga
     1021 acgaactcgc cgcgccggtg atctacagtg ccggaccgaa aggcgccacg aaccaactgg
     1081 tcgccacgct ctcgggctat tcctcgcctg gcacccgggt gttggtcagc ggggggggcg
     1141 ctccatttac attcccggct tcgggcgcaa tcgacgccac gggcttgttc actttcgagg
     1201 tgccattgca gctcaatgcg cagaactcga tcgtcctgcg agccagcgac agcgagggtc
     1261 gcctttccaa ccccgtgacc gcgctcgacg tccgacagga tgcgacgccg ccggacgtgg
     1321 tcgcgacgct gcccacggcg ggtgcgatca acgtcgacca gcatgctccg cttttcgtgg
     1381 agttttccga agccattcaa ccgggcccgc tcgcggcatc gatcccggcg attcgcttgt
     1441 tcgatgccca aaacgcgctt gtaaccggca gttggatact ctccgcggac agccgcagcg
     1501 ctacgttcta cgcgacgcgc agccttgcac ctgaggcgaa tttcaagctg ctcgtcggca
     1561 cggtggtccg ggatctcgcc ggcaacgccc tcgtggcgcc gctcgaggtg gcgttcacaa
     1621 cggcagcggc gggcgccgtc gatcggcctg cgacgccggt gctcgatccg ctggtttcaa
     1681 cgcgcacgac cgaatccgcc atcacgctcc gcgggagcgc catgccggga gcgcagttgc
     1741 gcgttttcgg cggtgaaaat ggcgccgcgg cggcggttgg agacgatggt cgctttacgg
     1801 ttgttgttcc actcgtgctc aacaccgcaa acccactcgc gatcgttgca gaggtgggcg
     1861 gcgccgtcag caatcctgcg gccatcacca tcgtccaagt cgagcatgcc gccgcggtcc
     1921 gcatcctgtc accgcaagcc ggcgttacct acaacaaccg gtcgctcacg gtcgcgggaa
     1981 ttatcgacga tccggaggca gtatctgcag tgaacgtggg cgggatcgcg gcttccattg
     2041 tcggacgcta tttcttcgcg caggttgtct tggatgaaac cgccggagcg aaatccgtga
     2101 cggcggttgc gacgctgaag gacggctcga cgctggaaac caccgtcgcg ttttcacttc
     2161 ttgtcgaggc cgccggcgtc gatacgcaac cgccgatccc gcgcttcctg tttccggaag
     2221 aaggcgatgt gcttaatggc gacgtcgtcg aaacgctcct gaccgtggag gagggcgttc
     2281 agctcacatc ggtcgagatt gatcgcgtcg tcgcgcatca ggtcgtcggt aatattttct
     2341 tcatccacgc gcggctcccg cagcaagggc tcaacgctat ttccgtcggc gccacggatg
     2401 ctgccggcca catcggcacg gccaccgtga acgtcgatgt cgactccatc ggtttcgcga
     2461 ccgcgcccac ggtcgcttca ctgccgtcgc tgacgaataa tcgagtgatc acgctgacgg
     2521 gcacggccga gcccgggagc accatcgtgg tgttgaatgg cctcgttccc gtgcgagtca
     2581 cggtgccggc ttccggagcc tatgtcgttt cggttccgct gaaccccaat gctgcgaacc
     2641 atctccaagt ggtcgcgacc gatgccgcgg gaaatctctc gccggtcacc accgtggaca
     2701 ttgttcacga cgatacgccg ccttccattg tcgcgacgtc acccagttca ggtcagaccg
     2761 gggtgccgca aaacgcgacg atcgaagtca ccttctcgga accactcaac cccgcttcga
     2821 ccgtctccga gacagcggtc gttgtgcgca gcgcaggcgg gcaagctatt caacgc
//
LOCUS       DASPLT010000378         1597 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_105731, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000378 DASPLT010000000
VERSION     DASPLT010000378.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1597)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1597)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1597
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_105731"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            322..>1597
                     /locus_tag="VEA63_03890"
     CDS             322..>1597
                     /locus_tag="VEA63_03890"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HYD83160.1"
                     /translation="MSTSFVSGLYSDFRDAFRQLRKGKGVTATTLLTLALCIGTTTAI
                     FSMVYALMLKPLPFHEPERIVELYSSAVKAGLNKMPTNVVMYLDYSKNATSYETLALW
                     TRYDGQMGEDGAIDRVGGAKATAEIFELLRVQPVLGSFFTKEQNREGEDKVVVLTRSF
                     WESKFGWDADVLGKTLRIDGETYRIIGVAPQVIEALDARVKFIVPFSWPPQAENPQGR
                     YGLNVQMLGRLKPGVAIGAADAEAKEIERRYYESSPPPVRQFVERSGITMNVGSVQAQ
                     RVEPVRPTLLLLQGGVAFVLLIGCVNVANLLLVRSNSRQGELAIRCALGAPRAAIARQ
                     LLVESLLLTGAGALLGMGLAWAALKGMNHYVQQMLPQALPITLDARVLVFAVVLTASV
                     GVLIGLIPITHVLRTNLTDVIQRSSRGASAGRG"
BASE COUNT          278 a          406 c          593 g          320 t
ORIGIN      
        1 gatcgtcgcg acaacggcca acgcgacggc ggacgatcgc acgcgctgca tgaaagtcgg
       61 gatgaacgac tatctgagca agccgctgcg gctggcggaa atggtgcggg tgatggagcg
      121 gtgggtggga tagccgccgc cggaggcggc ggaacatcca agcttcaaga tccaagctcc
      181 agggagtttt caactcccaa ccatcagccc gtttgtgttc gagacttgga gcttgggatt
      241 tgaagattcc ctggagcttg gatcttggag cttggagctt tgtaaccctc ggggtagtcg
      301 tggggttgtt accccgcccc catgagcact tcatttgttt ccggcttgta ttcggatttt
      361 cgtgacgcgt ttcggcagtt gagaaaaggg aaaggcgtca ctgcgaccac gctcctcacg
      421 ctcgcgctat gcatcggcac gacaacggcg attttctcga tggtgtatgc gctgatgttg
      481 aaaccgctgc cgtttcacga accggagcgg atcgtggagc tgtattcgtc ggcggtgaag
      541 gcggggctga acaagatgcc gacgaacgtc gtgatgtatc tcgattattc gaagaacgcg
      601 acgtcgtatg aaacgctggc gttgtggacg cgctacgacg ggcagatggg cgaggatggg
      661 gcgatcgatc gcgtgggcgg cgcgaaggcg acggcggaaa ttttcgagtt gctgcgcgtg
      721 cagccggtgc tcgggagttt tttcacaaag gagcagaacc gcgagggcga ggacaaggtg
      781 gtggtgctga cgcgttcgtt ttgggaatcg aagttcggct gggatgcgga cgttctcgga
      841 aagacgctgc gcatcgatgg agaaacctac cggatcatcg gcgtggcgcc gcaggtgatc
      901 gaggcattgg acgcgcgcgt gaagtttatc gtgccgtttt cgtggccgcc gcaggcggag
      961 aatccgcaag ggcgctatgg gctgaatgtg cagatgctcg ggcgattgaa accgggcgtc
     1021 gcgatcggcg cggcggatgc ggaagcgaag gagatcgagc ggcgttacta cgagagttcg
     1081 ccgccgccgg tgcggcagtt tgtcgaacgc tcgggcatca cgatgaacgt cggcagcgtg
     1141 caggcgcagc gggtggagcc ggtgcggccg acgctgctgt tgttgcaagg cggcgtggcg
     1201 ttcgtgctgc tgatcggctg cgtgaacgtg gcgaacctgc tgctcgtgcg ctccaactcg
     1261 cggcaggggg aactggcgat ccgttgtgcg ctcggcgcgc cgcgcgccgc gatcgcgcgt
     1321 cagttgctgg tggagagcct gctgctgacg ggcgcgggcg cgctgctcgg catggggctc
     1381 gcatgggcgg cgttgaaggg gatgaatcat tatgtgcagc agatgctgcc gcaggcgctg
     1441 ccgatcacgc tggatgcgcg cgtgctggtc tttgcggtgg tgttgacagc gagcgtgggc
     1501 gtattgatcg gactgatacc gatcacgcat gtgctgcgga cgaatctgac cgacgtgatc
     1561 cagcgttcct cgcgcggggc gtcggcgggg cgtggcg
//
LOCUS       DASPLT010000379         1612 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_27204, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000379 DASPLT010000000
VERSION     DASPLT010000379.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1612)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1612)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1612
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_27204"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..485)
                     /locus_tag="VEA63_03895"
     CDS             complement(<1..485)
                     /locus_tag="VEA63_03895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD83161.1"
                     /translation="MVPDARSHRRLFWQLQIGGWLLMVTIATGFVSLAFNDWTNIALA
                     GVGRQIFAFFLTLGLWRFYRRWPAETFKLAPHALQIALACLTVTVLEGMLVEAIRRAF
                     DLPSVPLLAQRGALPSRLSLYLAWTSLYFMIRQELAARDRGLRLAHAETAAREAELAT
                     LR"
     gene            complement(573..>1612)
                     /locus_tag="VEA63_03900"
     CDS             complement(573..>1612)
                     /locus_tag="VEA63_03900"
                     /inference="COORDINATES: protein motif:HMM:NF017745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF885 domain-containing protein"
                     /protein_id="HYD83162.1"
                     /translation="LVEHYIPAARETTAAVDLPDGEAWYRHQIKVMTSTELDARQIHE
                     IGLAEVARIRAEMERVKTESGFEGTLEAFFVHLRTAPEFYFTDAEELVRAYRDIAKRA
                     DPLLIKFFKTLPRTPYGVLPVPSYAEQSQTTAYYYPGSPEAGRPGYFFANTYALHTRP
                     KWEMEALTLHEAVPGHHLQIALAQELPNVPEFRKWGGYTAFVEGWGLYAEHLGEEMGF
                     YADPYSKFGQLTYEMWRAVRLVVDTGLHALGWTREEAIEYFAKNASKSEHDIVVEIDR
                     YLVWPGQALAYKLGELKIKELRARATKELGAKFDVREFHDEVLRHGAVPLSVLEDNID
                     AWVAAQAAGGR"
BASE COUNT          316 a          567 c          457 g          272 t
ORIGIN      
        1 cgaagcgtgg cgagctcggc ttcgcgcgcg gcggtctcgg cgtgggcgag ccggagcccg
       61 cggtcgcgcg ccgcgagttc ctgtcgaatc atgaaataga gcgaggtcca cgccagataa
      121 agcgagagac ggctgggcag cgcgccacgt tgcgcgagga gcggcacgga gggcagatcg
      181 aacgcacggc ggatcgcctc gaccagcatg ccttcgagca cggtcacggt caggcaggcg
      241 agcgcgatct gtagggcatg aggcgcgagc ttgaaggtct cggccggcca gcggcgataa
      301 aagcgccaga gtccgagcgt cagaaaaaat gcgaagatct gccgtccgac gcccgccagc
      361 gcgatgttgg tccaatcatt gaaggccagc gagacgaagc cggtggcgat cgtcaccatc
      421 agcagccagc cgccgatctg caattgccag aagagccggc gatgggagcg tgcgtcggga
      481 accacagcga cgaaattgag cgcgcgaaag ctcaatgcga acgcaaagaa gcgatgaacc
      541 gcattctcgc cgcctccgat taaaacgccg ccctacctcc cgcccgccgc ctgcgccgcg
      601 acccacgcgt cgatgttgtc ctccagcacg ctcaacggca ccgcgccgtg ccgcagcact
      661 tcgtcgtgaa actcccgcac atcgaacttc gcaccgagct ctttcgtcgc gcgcgcccgc
      721 agttccttga ttttcaactc gccgagtttg taggcgagcg cctgtcccgg ccatacgagg
      781 taacgatcga tctccaccac gatgtcgtgc tccgacttgc tcgcgttctt cgcaaaatac
      841 tcgatcgcct cctcgcgcgt ccacccgagc gcgtgcaggc ccgtgtcgac caccagccgc
      901 accgcccgcc acatctcata cgtgagttgt ccaaattttg aatacgggtc ggcatagaat
      961 cccatctctt cgccgaggtg ctccgcgtaa agtccccacc cttcgacgaa cgccgtgtaa
     1021 ccaccccact tccgaaattc cggcacgttc ggcagctcct gcgcgagcgc gatctgcaag
     1081 tgatgccccg gcaccgcctc gtgcagcgtc aacgcctcca tttcccactt cggccgcgtg
     1141 tgcagcgcgt aagtattcgc aaaaaaatat ccgggccgcc ccgcctcggg cgaacccggg
     1201 taataatacg ccgtcgtctg cgactgctcc gcatagctcg gcaccggcag cacgccatac
     1261 ggcgtgcgcg gcagcgtctt gaaaaatttg atcaagagcg ggtccgcccg tttcgcgatg
     1321 tcgcgatacg cgcgcaccaa ttcctccgca tccgtgaaat aaaactccgg cgccgtccgc
     1381 aggtgcacga agaacgcctc gagcgtccct tcaaacccgc tctccgtttt cacgcgctcc
     1441 atctccgcac ggatccgcgc cacctccgcg agaccgatct cgtgaatctg ccgcgcgtcg
     1501 agctccgtcg acgtcatcac tttgatttga tgccgatacc acgcctcgcc atccggcaaa
     1561 tccaccgccg ccgtcgtctc ccgcgccgcg gggatgtaat gctcgaccaa at
//
LOCUS       DASPLT010000380         2223 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_250535, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000380 DASPLT010000000
VERSION     DASPLT010000380.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 2223)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2223)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2223
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_250535"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..289
                     /locus_tag="VEA63_03905"
     CDS             <1..289
                     /locus_tag="VEA63_03905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HYD83163.1"
                     /translation="GIASSGDYLRGFAIGGRRYGHIVDPRTGWPVANGCVQATAIAAT
                     CLHAGVLSTTAFVLGVQKGIEFIQSFPGAEGLIVTQQARAQTRGFFHHVVS"
     gene            300..824
                     /locus_tag="VEA63_03910"
     CDS             300..824
                     /locus_tag="VEA63_03910"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HYD83164.1"
                     /translation="MRRATRGGIIHAVVRRATISLLALATFARAQIAVGDAFPSLADA
                     ELTGGELPAIEGKVVLVDFWASWCAPCKASFPAMAKLHADYAARGLAIVAVSVDEKAA
                     SYAGFLKRMKPPFATLRDERHKLVRAVNLPAMPTSYLLGRDGRVRFVHRGFHGASSEE
                     ELRKNIEALLAENS"
     gene            834..1073
                     /locus_tag="VEA63_03915"
     CDS             834..1073
                     /locus_tag="VEA63_03915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4266 domain-containing protein"
                     /protein_id="HYD83165.1"
                     /translation="MKTPAKTTILTFLMLALGLFAGAGCSTVRVQPWERAALSDYAMR
                     PDRDPLAGVSTDHTYFSREAAHGGRGVGGSGCGCN"
     gene            1178..1861
                     /locus_tag="VEA63_03920"
     CDS             1178..1861
                     /locus_tag="VEA63_03920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HYD83166.1"
                     /translation="MRIHVIEDEAQLARHIGRALERHGHVASVKLDGAEGLNAAISDP
                     PDLIVLDLNLPSLDGMSVLARLREANNPARVLILTARGEVEHSVKGLKAGADDYLAKP
                     FSLDELVARVEALGRRGATPTAADFLKVGDLQMDVQHRRVMRAGRPVALSPREFDVLQ
                     VLMQEPGRVFSRTELCERVWQRDHEYDTRTVEIFIARLRKKVDTGFPHPLIHTLRSVG
                     YTVRSLASS"
     gene            1858..>2223
                     /locus_tag="VEA63_03925"
     CDS             1858..>2223
                     /locus_tag="VEA63_03925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD83167.1"
                     /translation="MSFQVFQRALLAVLIGTAMTLRAETEIVGRVALPKEREAPVMNK
                     RYEIVSRGGVIATNPPLAVVYVEGDFADSAQQSVVQLPQKDLAFVPALLPVRVGTVVE
                     FPNLDDTYHNIFSYSPPKRF"
BASE COUNT          370 a          665 c          744 g          444 t
ORIGIN      
        1 cggcattgcg tcgtcgggcg attatctgcg cgggttcgcg atcggtggcc ggcgatatgg
       61 ccacatcgtc gatccgcgca ccggctggcc cgtggcgaat ggttgcgtgc aagcaacggc
      121 gatcgctgcc acgtgcctgc atgcaggcgt gctgtcgacg acggcattcg tgctgggtgt
      181 gcagaaaggc atcgagttca tccagtcgtt ccccggtgcg gagggtctga tcgttacgca
      241 gcaagcgcgc gcgcaaaccc gcggattttt tcaccatgtg gtttcttgat acatttcccg
      301 tgcgccgggc tactcgcggc ggaatcatcc acgcggtggt gcgcagagcc accatctcgc
      361 tgctggcgtt ggccacgttt gcgcgcgcac agatcgcggt gggcgacgca tttccctcgc
      421 tggctgacgc cgagctcacg ggcggcgagt tgccggccat cgaaggcaag gtcgtgctcg
      481 tcgacttttg ggcgtcatgg tgcgcgccgt gcaaagcgtc gtttcccgcg atggcgaaac
      541 tccacgccga ctacgcggct cgcggactgg cgatcgtcgc ggtgagcgtg gatgagaaag
      601 cggcgtcgta tgcgggtttt ctcaaaagaa tgaaaccgcc cttcgccaca ctgcgggatg
      661 agcggcacaa gctcgttcgc gcggtgaacc tgcccgcaat gccaacgtcc tatttgctcg
      721 gtcgcgacgg ccgcgtgcga tttgtgcatc ggggatttca cggtgcctcg agcgaagagg
      781 agttgaggaa aaacatcgaa gccctgctgg cggagaacag ctgaccgctc gtcatgaaaa
      841 cacctgcgaa aaccacgatt cttacttttt tgatgctggc gctcgggctg ttcgcaggcg
      901 ccggttgcag cacggttcga gtgcagccgt gggaacgcgc cgcgctctcc gattacgcga
      961 tgcggcctga tcgcgatccg ctcgccggcg tgtcgacgga tcacacttat ttttcgcgcg
     1021 aggcggcgca cggcgggcgc ggcgtcggcg gcagcggctg cgggtgcaat tgagcgaagc
     1081 ttgcgcgccg ttcgcatttg gagaaactca cgccgtcgtc gccgttgccc gcgattgccg
     1141 cccgatccgt attttccccg tctcgaaatt tcaccgcatg cgcattcatg tgatcgagga
     1201 cgaagcccag ttggcgcggc acatcggccg tgcgctcgag cggcatggtc acgtcgcgag
     1261 cgtgaagctc gacggcgccg aaggtctcaa cgccgcgatc tccgatccac ccgatctgat
     1321 cgtgctcgat ctcaacttgc ctagtctcga tggcatgtcg gtattggcgc gattgcgcga
     1381 agcgaacaat ccggcgcgcg tgctgatcct caccgcgcgc ggcgaagtgg aacacagcgt
     1441 gaagggcttg aaagcgggcg cagacgatta tctggcgaag ccgttttcgc tcgatgaact
     1501 cgtcgcgcgc gtcgaggcgc tcggccggcg gggcgcgaca ccgacggccg cggatttcct
     1561 gaaggtgggc gatctgcaaa tggatgtgca gcatcgccgc gtgatgcgtg ccggccggcc
     1621 ggtggcgctt tcgcctcgcg agttcgacgt cttgcaggtg ctgatgcagg agcccggccg
     1681 cgtgttttcg cggacggagt tgtgcgaacg tgtctggcag cgcgatcacg aatacgacac
     1741 gcgcacggtc gagatcttca tcgcgcggct ccgcaagaaa gtggacacag gttttcctca
     1801 tccgctgatc cacaccttgc gttccgtggg ctacaccgtg cgcagcctgg cgtcatcatg
     1861 agcttccagg tgtttcaacg ggcgttgctc gctgtgctga ttggcacggc gatgacgttg
     1921 cgggccgaga cggagatcgt cgggcgcgtc gcgctgccga aagagcgcga ggcgccggtc
     1981 atgaacaaac gctacgaaat cgtcagccgc ggcggcgtga tagcgacgaa tccgccgctc
     2041 gcggtcgttt atgtcgaggg cgattttgcg gactcggctc agcagtcagt ggtgcagttg
     2101 ccgcagaaag acctggcgtt tgtgccggcg ctgctgccgg tgcgagtcgg cacggtggtg
     2161 gagtttccga atttggacga cacgtatcac aacatctttt cctactcgcc accgaagcgg
     2221 ttc
//
LOCUS       DASPLT010000381         2443 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_27214, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000381 DASPLT010000000
VERSION     DASPLT010000381.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 2443)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2443)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2443
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_27214"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..450
                     /locus_tag="VEA63_03930"
     CDS             <1..450
                     /locus_tag="VEA63_03930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012377323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThuA domain-containing protein"
                     /protein_id="HYD83168.1"
                     /translation="WVYWKERIVRDPSPGRGGDHGAIHAFQLVSRQPDHPIMRGLPAA
                     WLHASDELYNQLRGPAENLTVLATAFDDPANRGTGRHEPILFTVDYGRGRVFHTTLGH
                     ARNGDVTALRCVGFIATFQRGAEWAASGKVTQLVPSDFPTATAVSTR"
     gene            640..1011
                     /locus_tag="VEA63_03935"
     CDS             640..1011
                     /locus_tag="VEA63_03935"
                     /inference="COORDINATES: protein motif:HMM:NF019491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dabb family protein"
                     /protein_id="HYD83169.1"
                     /translation="MKKLLMSLVTAGLLLGATATMQAGESAPKSVIHVVTVSWKEGTS
                     PEQIQAALDGVKALPSKFKGITRVWTKSVKVQGGKANAFVMEFADEAALKDYSGSDAQ
                     KEWYKVYTPIRESSTTFDISN"
     gene            1204..1974
                     /locus_tag="VEA63_03940"
     CDS             1204..1974
                     /locus_tag="VEA63_03940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012377311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThuA domain-containing protein"
                     /protein_id="HYD83170.1"
                     /translation="MIQVKRFTLFLLLLATAAAARAEQFSVALFTKTVGWHHDSINAG
                     VTAIQALGEQHDFKVFWTEDANRIFTDKELANYKAVIFLLTTGDVLDPEQQAAFERYI
                     RGGGGFVGVHSASDTEYDWPWYTKMVGHMFHIHPAVQTATIEVKDPNFPGMDRFAKRF
                     LFTEEWYEFGPPTSDQLKHLLVVDEKTYQPAADWGAKKGDGMGDLHPVAWYQNYDGGR
                     AFYTALGHLPASYRDANFLHHIYGGIYWAATGNGFRAE"
     gene            complement(2002..>2443)
                     /locus_tag="VEA63_03945"
     CDS             complement(2002..>2443)
                     /locus_tag="VEA63_03945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HYD83171.1"
                     /translation="VSVFSVGRANAIDQSLFRADVTYDGVADIQAIGVQSGSGRFGGI
                     RAGNAELWGTTGWVGINATRVSFEGPVRIGNLSASDAAEPMLRMETGAGANVDIAGGD
                     LLQENARAIHLDGAITVTAIAGEASSGEARPTQVLRGTFRNDRR"
BASE COUNT          464 a          929 c          613 g          437 t
ORIGIN      
        1 tgggtctatt ggaaagaacg catcgttcgc gacccctcgc ccggccgcgg cggcgatcac
       61 ggcgccatcc acgcgtttca actcgtctcg cgccagccgg accacccgat catgcgagga
      121 ctccccgctg cgtggctcca cgccagcgac gaactttata accaactgcg cggtcccgcg
      181 gaaaacctca ccgtcctcgc caccgccttc gacgatcccg cgaaccgcgg caccggccgc
      241 cacgaaccca tcctctttac cgtcgactac ggtcgcggcc gcgtgtttca cacgacgctc
      301 ggtcacgcgc gcaatggcga cgtcaccgcc cttcgctgcg tcggcttcat cgccacgttt
      361 caacgcggcg ccgaatgggc cgcgtccggc aaagtcaccc aactggtccc ttcggatttc
      421 ccaaccgcca ccgccgtttc gactcgctag ccgtgcgatc ggcctctttg catcaccacg
      481 ccggtgttgg aaaccgctgc gccttttcta tgacggtcgt ttcacgcaac caatcccgcc
      541 tcgaaccgtc cataccccct tgccgctgac accgtgtcgg cggcttcgtc cggcggccaa
      601 tcgcccggac atcaacccca ccctcccagg agaataccaa tgaaaaaact gctcatgtcc
      661 ctcgtcaccg ccggcctgct gctcggcgcc actgccacga tgcaagccgg tgagtccgct
      721 cccaagtccg tgatccacgt ggtcaccgtc tcgtggaaag aaggcaccag ccccgaacaa
      781 atccaggccg cactcgacgg cgtcaaagcc ctgccgtcga agttcaaggg catcacccgc
      841 gtctggacca agtcggtcaa agtccagggc ggcaaagcca acgccttcgt catggagttc
      901 gccgacgagg ccgcgctgaa ggactactcc ggcagcgacg cgcaaaaaga atggtataag
      961 gtctacacgc cgatccgcga atcgagcacg accttcgata tctccaactg atctgcgcgc
     1021 gctttcattc gcgcacgctc agcaggttcc agggccgcca tccggcggcc cttttttatt
     1081 tcacttgccg gaactcaccc gcgatcacct caccgcgcca ctgactcacg caccgtcgcc
     1141 agcatttccg cgtgcccttg cctttgcctc gccacggacg tcttccccat tttcctcgcc
     1201 atcatgatcc aagttaaacg cttcacgctc tttctcctcc ttctcgccac cgctgccgcc
     1261 gcccgcgccg agcaattcag cgttgcgctc ttcacgaaga ccgtcggctg gcatcacgac
     1321 tccatcaacg ccggcgtcac cgccattcag gcgctcggcg aacagcacga cttcaaagtt
     1381 ttctggaccg aggacgccaa ccgcatcttc accgacaagg agctggcgaa ctacaaagcc
     1441 gtcatcttcc tgctcaccac cggcgacgtt ctcgatcccg agcagcaagc cgccttcgag
     1501 cgctacattc gtggcggcgg cggctttgtc ggcgtccaca gcgcgtccga caccgaatac
     1561 gattggccat ggtatacgaa gatggtcggc cacatgtttc acattcatcc cgcggtccag
     1621 accgccacga tcgaggtgaa agaccccaac ttccccggta tggatcgctt cgcgaaacgt
     1681 ttcctcttca ccgaagaatg gtatgagttc ggcccgccga cctccgatca gttgaagcac
     1741 ctcctcgtcg tcgacgagaa aacctaccag cccgccgccg actggggcgc gaaaaaaggt
     1801 gacggcatgg gcgacctcca tccggtcgca tggtatcaaa attacgacgg cggacgcgcc
     1861 ttctacaccg cgctcggaca cctgccggcc agttaccgcg acgcgaattt cctccaccac
     1921 atttacggcg gcatctactg ggccgcgacg ggcaacggct tccgcgccga ataaaatccc
     1981 gagctcaaac gccccgacgc gtcaccgccg gtcgtttcga aacgtcccgc gcaacacctg
     2041 cgtgggacgc gcctctccgc tcgaagcctc gccagcgatc gccgtcaccg tgatcgcacc
     2101 gtcgaggtga atcgcccgcg cgttttcctg tagaagatcg ccgcctgcga tatcgacgtt
     2161 cgcacccgcg cccgtttcca tccgcaacat cggctccgcg gcatcgctcg ccgacagatt
     2221 cccgatccgc accggccctt cgaaactcac gcgcgtcgcg ttgatcccga cccacccggt
     2281 cgttccccag agctccgcat tcccagcgcg aatgccgccg aaccggccgc tgccgctctg
     2341 cacgccgatc gcctgaatgt ccgccacgcc gtcgtaggtc acgtccgcgc gaaacaaact
     2401 ttgatcgatc gcattcgccc gccccaccga aaacacgctc aca
//
LOCUS       DASPLT010000382         1219 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_189371, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000382 DASPLT010000000
VERSION     DASPLT010000382.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1219)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1219)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1219
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_189371"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..618
                     /locus_tag="VEA63_03950"
     CDS             <1..618
                     /locus_tag="VEA63_03950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HYD83172.1"
                     /translation="QVPVRQEIGMTFASALRAMLRQAPNVVMIGEIRDFETAEIAINA
                     SMTGHMVFSTLHTNDAPGAVTRLMDLGVKPFLVATSLRATMAQRLVRKICEKCKQPHV
                     PSERELRALGIDVKSLATAAFARGAGCAVCNGTGYRGRVGIFEIFAVDEELQRMIHER
                     ASGARLRARAREFGMRTMREDGARKASAGLTTIEEVVSITAADAA"
     gene            724..>1219
                     /locus_tag="VEA63_03955"
     CDS             724..>1219
                     /locus_tag="VEA63_03955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007358651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HYD83173.1"
                     /translation="MSYEMNELLDLVVEQNASDLHLQVGQPPTLRLSGSMTPIEGPNL
                     TPADTEKLMLSITPDSHISNVKLNGGSDFGFAFGDKARFRVSVLKAKGNYGMVLRQIP
                     YKMFGLRDIGLPDKIRELLYRPRGLILVTGPTGSGKSTTLASMINYVNEHRDGHIITI
                     EDPIE"
BASE COUNT          248 a          302 c          431 g          238 t
ORIGIN      
        1 caggtgccgg tgcggcagga gatcgggatg acgtttgcgt cggcgttgcg ggcgatgctc
       61 cggcaggcgc cgaatgtggt gatgatcggt gaaattcgtg atttcgaaac ggcggagatc
      121 gcgatcaacg cgtcgatgac gggacatatg gttttctcga cgttgcacac gaacgatgcg
      181 ccgggggcgg tgacgcgatt gatggatctg ggcgtgaaac cgtttctggt ggcgacgtcg
      241 ttgcgggcga cgatggcgca gcggctggtc cgaaaaattt gtgaaaagtg caagcagccg
      301 cacgtgccgt cggagcggga gttgcgggcg ttgggcattg acgtgaagag tttggcgacg
      361 gcggcgtttg cgcgcggagc aggttgcgcg gtgtgtaacg gcacgggata tcgcggtcgg
      421 gtggggattt tcgaaatctt cgcggtcgac gaggagttgc agcggatgat ccacgagcgg
      481 gcgagcggag cgcgtttgcg ggcgagagcg cgagaattcg gcatgcgcac gatgcgcgaa
      541 gacggagcgc gcaaggcaag cgctggtttg accacgatcg aggaagtcgt ctcgatcacg
      601 gccgccgatg cggcttagaa agttccaagt cacaagtatc aagtagcaag tatcacgctc
      661 cacctgcagt gcggccgagc gagtcggtcg gtttgatcag gagcgaaaca ccttttttcc
      721 agcatgagtt acgaaatgaa cgaactgctc gatcttgtcg tcgagcaaaa cgcctccgat
      781 ctccacttgc aggtcggtca gccgccgacg ttgcggttga gcggcagcat gacgccgatt
      841 gaaggaccga acctgacgcc ggccgacacg gagaagctca tgttgtcgat cacgccggac
      901 agccatatca gcaatgtgaa gctgaacggc gggtcggact tcggattcgc gttcggcgac
      961 aaggcgcggt ttcgcgtgtc ggtgctgaag gcgaagggga actacggcat ggttctgcgg
     1021 cagatcccct acaagatgtt tggtctgcgc gatatcggcc tgccggataa aattcgcgag
     1081 ctgctctatc ggccgcgcgg gttgattctc gtgacgggcc cgacagggtc gggcaagtcg
     1141 acgacgctcg cgtcgatgat caattacgtg aacgagcatc gcgatggtca catcatcacg
     1201 atcgaggatc cgattgagt
//
LOCUS       DASPLT010000383         2447 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_27234, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000383 DASPLT010000000
VERSION     DASPLT010000383.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 2447)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2447)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2447
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_27234"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..335)
                     /locus_tag="VEA63_03960"
     CDS             complement(<1..335)
                     /locus_tag="VEA63_03960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007361739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HYD83174.1"
                     /translation="MPRNVTLFTGQWADLPLTELAPLAKKMGYDGLELACWGDHFDVD
                     AAASSKKYLAEKWQLLADHGLTCFAISNHLVGQAVCDNIDERHKAILSPAIWGDGSPE
                     GVRKRAAKQL"
     gene            574..1320
                     /locus_tag="VEA63_03965"
     CDS             574..1320
                     /locus_tag="VEA63_03965"
                     /inference="COORDINATES: protein motif:HMM:NF015506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HYD83175.1"
                     /translation="MKARSFILMLAALGIATTAWSAQPVNYTPMKVIQTFTPVYPRSL
                     ADIGIKQGETHVSIQVDEQGQLTDHLVTYYTHAAFAESATQALKKWKYEPAWLDGEAR
                     SATVDLTFIYENQGLTVVNLDPGVYVMRRSMELMPGTFGYRVARLRELDRIPTPKKVV
                     SPPYPVDASGSPRAAKVTVTFFIDEEGRVRLPAVSRDATEGNELFASAAITAVSQWEF
                     EPPTANGRRALVQVRQDFNFNPTTATKQAE"
     gene            complement(1662..2270)
                     /locus_tag="VEA63_03970"
     CDS             complement(1662..2270)
                     /locus_tag="VEA63_03970"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HYD83176.1"
                     /translation="MTTDPATDRASLAALQQGDTTALNRLIARWQRPLLSYAYRYVQN
                     SADAHDLVAGVFVRFYQQRLKLRADTNVSAWLFTALTNLCHNHYRWKRRHPTVPLDPV
                     SRDDAGDRPTTAPADLASEQPNPLAVLAQDELQDAVRSAIGRLPHDLKVAVLLHHYQN
                     LSYREIAEISGCSERGVETRLYRARQQLRQGLSTFIREASSC"
BASE COUNT          420 a          653 c          904 g          470 t
ORIGIN      
        1 agttgtttgg cggcgcgctt gcggacgcct tcgggagaac cgtcgcccca gatggcgggc
       61 gagagaatgg ctttgtgacg ttcgtcgatg ttgtcgcaga ccgcctggcc gacgaggtga
      121 ttggaaatgg cgaagcacgt caggccgtga tcggcgagga gctgccattt ttcggcgagg
      181 tattttttgg acgaggccgc ggcgtcgacg tcgaagtggt cgccccagca ggcgagctcg
      241 aggccgtcgt agcccatttt tttcgcgagc ggcgcgagtt cggtgagggg aagatcggcc
      301 cactggccgg tgaagagtgt gacgtttctt ggcatggcag gaaggggttg agaacgtgcg
      361 agacggcgcg caagacgtgc cggtggagta ttggtttcgc gtgcgacgcg gagcgcggca
      421 agggcgaatt tacgcgaggc ggtgggttac gacccgcggc atcgaatcga aggagcgccg
      481 ggggttgcgg cgcctcgggt cgcgcgcttt cgtggccggt gacgtgaagg gacgaggggg
      541 cgttcggtgt tatgcgggca actctacttt atcatgaagg cacgctcttt tatcctgatg
      601 cttgcggcgc tcggaatcgc gaccaccgcg tggtcggccc agcctgtgaa ctacacgccg
      661 atgaaggtca ttcagacctt caccccggtt tatccccggt cgctcgccga tatcggcatc
      721 aaacaaggtg agacgcacgt ttccatccaa gtcgacgagc agggtcagct cacggatcat
      781 ctggtcacgt attacacgca cgccgctttc gcggagagtg cgacgcaggc cttgaagaag
      841 tggaagtatg agccggcgtg gctcgatggc gaagcgcgga gtgcgacggt cgacctgacg
      901 ttcatttatg agaatcaggg cctgacggtg gttaacctgg acccgggcgt gtatgtcatg
      961 cgtcgctcga tggagctgat gcctgggact ttcgggtatc gcgtggcgag attgcgcgag
     1021 ctcgatcgga ttcccacgcc gaagaaggtg gtgtcgccgc cgtatccggt cgatgcgtcg
     1081 ggctcgccgc gggcggcgaa ggtgacggtg acgtttttca tcgatgagga aggccgggtg
     1141 cgtttgccgg cggtgagccg cgacgcgacg gaggggaacg agttgttcgc gtcggcggcg
     1201 atcacggcgg tgtcgcagtg ggaattcgaa ccgccgacgg cgaatggccg gcgcgcgctc
     1261 gtgcaggtgc gccaggattt caatttcaac ccgacgactg cgacgaagca ggctgaataa
     1321 tttgctttgg tatgtgtgcg agcccggcac ggggtgtgcc gggctttttt gtttcggctt
     1381 tcgggcgatg gcaaaacgcg gaaatggcgg aggtggcgac gagccagcgc ggctcgagcc
     1441 gccgccgccg cgctctcgca agttcgagaa gttcgagagc atatttgaaa aactgtagcc
     1501 gtttctccgt agcggcaggc gtccctgcct gctcgttttc gggaaggtag gtggtgcgct
     1561 tgcgcaccac gttgcgcgcg agcgcaaccc acggcgggca ggcaggcgcg gacgcctgcc
     1621 gctacattcg acggctacgt ttttatttaa accgctgtaa tctagcagct ggaggcttcg
     1681 cggatgaacg tgctcaggcc ctggcggagt tgctggcggg cgcggtagag acgcgtttcg
     1741 acgccgcgtt cggagcagcc ggaaatctcg gcgatctcgc gatacgagag attttggtag
     1801 tggtgcaaca agaccgcgac cttgagatcg tgcggcagcc ggccgatggc gctgcgcacg
     1861 gcgtcctgca actcgtcctg cgcgagcacg gccaacgggt tcggctgttc gctggcgaga
     1921 tcggcgggcg cagtcgtggg gcgatcgccg gcgtcgtcgc gcgaaaccgg atcgagcggc
     1981 acggtgggat ggcggcgctt ccagcgatag tggttgtggc agagattggt gagcgcggtg
     2041 aacagccagg cggacacgtt tgtgtcggcg cgcagcttaa gccgttgctg gtaaaaacga
     2101 acgaacacgc cggcgacgag atcgtgagcg tcggcggaat tttgcacgta gcgatacgcg
     2161 tagctgagca acgggcgctg ccagcgcgcg atcaaccggt tcaacgccgt ggtgtcgccc
     2221 tgctgcaagg cggcgaggct ggcgcgatcg gtggcggggt cggtggtcac ggcgcgcggg
     2281 gtgagcgctc agttgatctg caaagcctgg tgtgattcga acgcggaaac gatgccgagg
     2341 taacgggcgc gctgctcggg cgtcatcacg cccgcggcgg cctgcacgag attgcgcgtg
     2401 gattcgaggc actcgcggtt gatgttgcgc cgcgtttcga caaagcg
//
LOCUS       DASPLT010000384         1199 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_27247, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000384 DASPLT010000000
VERSION     DASPLT010000384.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1199)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1199)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1199
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_27247"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1171
                     /locus_tag="VEA63_03975"
     CDS             <1..1171
                     /locus_tag="VEA63_03975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NirA family protein"
                     /protein_id="HYD83177.1"
                     /translation="ALHHYILNHRDLYGLPRKFNISFDNGGSIASAADTNDIGFMAVK
                     VTHASLAKLGATGAASSPIASTLPSPGIYFRVQLGGISGHGDFARDTGLLVKPSECVA
                     VATAMVRVFNENGDRTNRKKARLKYLLEKWGTEKFLAETQRKLAFPLERLPLEACESR
                     RAEIKHGWVGVYKQSQRGLNSIGLGVPVGRLSAKQMRQIADLAANYGKGEVRLTVWEN
                     IIVPHVPDAFVATACRSFQRLGFATEAHSLTSGIISCTGSKGCKYSSTDTKAHAVALG
                     KALAGKKLSIEQPLNLHFTGCPHSCAQHYCGDIGFLGAKLADGGEGYHVVLGGGMSQE
                     QGIAREVFRGIRAEELPTLTEKIVRTYETKKHPGETFVQWTRRHTLKELQELLSE"
BASE COUNT          245 a          413 c          342 g          199 t
ORIGIN      
        1 agcgctgcac cactacatcc tcaaccaccg cgacctctac ggcctgccga ggaaattcaa
       61 catctccttc gacaacggtg gctcaatcgc cagcgccgcc gatacgaacg acatcggctt
      121 catggccgtc aaggtcaccc acgcttcgct ggcaaaactc ggcgcgaccg gtgccgcttc
      181 gagccccatc gcctccacgc tgccctcgcc cggcatttac ttccgcgttc agctcggcgg
      241 catctccggg cacggggatt tcgcccgtga caccgggctg ctcgtcaagc cctccgagtg
      301 cgtggccgtc gcgaccgcga tggtgcgcgt tttcaacgaa aacggtgacc gcaccaaccg
      361 gaaaaaagcc cgcctcaaat atctgctcga aaaatggggc acggaaaaat tcctcgccga
      421 aactcagcgg aagcttgcgt tcccgctcga acgcctgccg ctggaggcct gcgagtcgcg
      481 tcgtgccgag atcaagcacg gctgggttgg cgtgtataaa caatcgcaac gcggcctcaa
      541 ctccatcggc ctcggtgttc ccgtgggccg gctctccgcg aaacagatgc ggcagatcgc
      601 cgatctcgcc gcgaattacg gcaagggcga ggtccggctc acggtatggg agaatatcat
      661 cgtgccccat gtgcccgacg cgttcgtcgc caccgcatgc cgcagtttcc aacgactcgg
      721 attcgcgacc gaggcgcaca gcctcacgag tgggatcatt tcgtgcaccg gcagcaaggg
      781 ctgcaaatac tcgtcgaccg acaccaaggc ccacgccgtc gcactgggca aagcgctcgc
      841 cggaaaaaaa ctttcgatcg agcagccgct caatctccac ttcaccggct gcccgcattc
      901 ctgcgcgcaa cattactgcg gcgatatcgg cttccttggc gccaagctcg ctgatggcgg
      961 cgagggctac cacgtggtgc tcggtggcgg catgagccag gagcaaggca tcgcccgtga
     1021 ggtgtttcgc ggcatccgcg ccgaggaact gccaacgctc acggaaaaga tcgtgcgcac
     1081 ctacgaaacg aaaaaacacc ccggcgagac gttcgtgcag tggacgcgcc gtcacacgct
     1141 caaggaactg caagaactcc tctccgagtg acggtgcgcg cgcgatgccg ccaccgtct
//
LOCUS       DASPLT010000385         1150 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_127155, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000385 DASPLT010000000
VERSION     DASPLT010000385.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1150)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1150)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1150
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_127155"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1150
                     /locus_tag="VEA63_03980"
     CDS             <1..>1150
                     /locus_tag="VEA63_03980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HYD83178.1"
                     /translation="LRIINGDVPESLKHKKLLALDMGSLIAGAKFRGEFEERLKAVLN
                     EVTSAEGGIILFIDEMHTLVGAGKADGAMDASNLLKPALARGELHCVGATTLDEYRKH
                     VEKDAALARRFQPVFVEEPSVEDSISILRGLKDRYEQHHGVRISDAAIVAAAQLSHRY
                     ITDRFLPDKAIDLMDEAASRLRMQVDSKPEELDELDRRTLQLKIELEALKKEKDPASQ
                     DRLKKLEAEIGQLETRSAELTAAWSAEKQKLQVTAKSKEELERAQKDYEDAVRRGDLA
                     RASELKYGRIPQLEKQIADAEKASGDGGGLVKEVVDAEAIAGVVSRWTGVPVEKMLEG
                     EKAKLLAMEDSLRGRVIGQEPALRAVADAVRRARAGLQDPNRPIGSFMF"
BASE COUNT          231 a          297 c          428 g          194 t
ORIGIN      
        1 ctgcgcatca tcaatggcga cgtgccggag agcttgaagc acaaaaagct gctggcgctc
       61 gacatggggt cgctgatcgc cggagcaaaa ttccgcggtg agttcgaaga gcggctgaag
      121 gcggtgctga atgaggtgac ctccgccgag ggcggcatca tcctgttcat cgatgagatg
      181 cacacgcttg tcggcgcggg caaagccgat ggcgcgatgg atgcgtcgaa ccttttgaag
      241 ccggctctgg cgcgcggcga gctgcattgc gtcggcgcga cgacgttgga tgagtatcgc
      301 aagcacgtgg agaaggacgc ggcgttggcg cggcgctttc agccggtgtt cgtcgaagag
      361 ccgagcgtgg aagactcgat ctcgatcctg cgcgggctga aggatcgtta cgagcagcat
      421 catggcgtcc gcatttcgga cgcagcgatt gtcgccgcgg cgcagctttc gcaccgctac
      481 atcaccgatc gcttcctgcc ggataaagcc atagatttga tggacgaggc ggcgtcgcgg
      541 ctgcggatgc aggtggattc gaagcctgaa gagttggacg aactcgatcg ccgcaccctg
      601 cagctgaaga tcgagttgga ggcgctgaag aaggaaaaag accccgcttc acaggatcgt
      661 ttgaagaagc ttgaagcgga gattggccag cttgagacgc gttcggcgga gctgacggcg
      721 gcgtggagcg cggagaagca gaagctgcag gtgacggcga aatccaaaga ggagctggag
      781 cgggcgcaga aagattatga ggacgcggtg cgccgcggcg atctcgcgcg cgcttcggag
      841 ctgaaatacg ggcgcatccc gcagctggaa aagcagatcg ctgacgccga gaaagcgagt
      901 ggcgatggcg gcggcttggt gaaggaagtc gtggacgccg aggcgatcgc cggcgtcgtc
      961 tcgcgctgga ccggcgtgcc ggtcgagaaa atgcttgagg gcgagaaggc caagctcttg
     1021 gcgatggaag attcgctgcg cgggcgcgtg attggccagg agccggcgtt gcgtgcggtg
     1081 gcggatgcgg tgcgacgcgc gcgtgcgggg ctgcaggatc cgaaccgacc gatcggctcg
     1141 ttcatgttct
//
LOCUS       DASPLT010000386         3375 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_5023, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000386 DASPLT010000000
VERSION     DASPLT010000386.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 3375)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3375)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3375
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5023"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..455)
                     /locus_tag="VEA63_03985"
     CDS             complement(<1..455)
                     /locus_tag="VEA63_03985"
                     /inference="COORDINATES: protein motif:HMM:NF018178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 16 glycoside hydrolase"
                     /protein_id="HYD83179.1"
                     /translation="MKRSPFTPRLGRTLLAFSLSFAASTHVFADEPTFVPLFNGKDLS
                     SFNTETSARFWRVENGVLIGENDPALKGDYLTTEKTYRDFIIEFDVRWTATTPRGVDT
                     GLEMRSPPIQLQLGVSGSLRVDMSGSFYTGGKPAYPEDGQAKDARTLMKP"
     gene            630..989
                     /locus_tag="VEA63_03990"
     CDS             630..989
                     /locus_tag="VEA63_03990"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HYD83180.1"
                     /translation="MSKTHKEILMEGNEAIARGDFEGFLVHCTENTVWNFLGDKTLRG
                     KEAVRRWMTEAYKEPPKFNVRELIADGEFLAAVGEITLKDENGKAAEFSYCDVWRFEG
                     SRMAELKAFVIAPKTNA"
     gene            complement(1330..2616)
                     /locus_tag="VEA63_03995"
     CDS             complement(1330..2616)
                     /locus_tag="VEA63_03995"
                     /inference="COORDINATES: protein motif:HMM:NF012253.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD83181.1"
                     /translation="MATVSRGLPEQPHIDVPKREARELLARWRAADPEALERIRQQIP
                     RYRAANDPALASAPIKLADAQFVIAREYGFANWAQLKLKIASTALTHQLRLAIHAGER
                     AAVVRMLRVHPELLHLPVWSGNWGPPMSHAANLGRLDLVQTLATLGARDFQHAFDRAL
                     LQGRIETARWLHAHGAKLVPGLVMGCCETLNVAGLGLLVELGAPFTDARGDRLAPLAL
                     ALETYARNPTGKHAVLAIFADQGYDLPDTPMMAFHRGQVDRLREFLQREPTLVHRRFA
                     AKEIYPAALGCAPDGRSGMCGTPIAGGTLLHLAIDFDERAIFELLLEHGADVNARASV
                     DAEGFGGHSPLFNAVVGSVFSGHGRRDGSMVRELLARGASTTLRANVRKFLDWQETPR
                     WHIAREVTPSQWARTFPHQDWVNVDALRLIEEAGAG"
     gene            2804..3124
                     /locus_tag="VEA63_04000"
     CDS             2804..3124
                     /locus_tag="VEA63_04000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD83182.1"
                     /translation="MAAERRGAALGAIALKDGHGKATEYSSCDVWRFENGRMAELTDA
                     VIALSRIHAEDLPRMTRMTANFREVESNVAASRPDHRLVKDHFSSSVFAAIGVIRGQL
                     NDRR"
     gene            3150..>3375
                     /locus_tag="VEA63_04005"
     CDS             3150..>3375
                     /locus_tag="VEA63_04005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD83183.1"
                     /translation="MQPTKSLVSLRPVVADDLPILFAHQRDPESVRLAAFVSRDHDAF
                     MLHWTTRILGNPAAACRTVLFDDNVVGYIGA"
BASE COUNT          655 a         1028 c         1075 g          617 t
ORIGIN      
        1 ggcttcatga gtgttctagc gtccttcgcc tgaccgtcct ccggataagc gggctttcct
       61 ccggtgtaaa acgaaccgga catatccacg cgcaagctgc ccgagacgcc gagctgcagt
      121 tgaatcggcg gactccgcat ctccagcccc gtgtcgacgc cgcgcggagt ggtggctgtc
      181 cacctcacgt cgaattcgat gatgaaatcg cgatacgttt tctccgtcgt gagataatcg
      241 cctttgagag cggggtcgtt ttcgccgatc agcaccccgt tctcgacccg ccaaaaacgc
      301 gccgacgtct ccgtgttgaa cgaggagaga tccttgccgt tgaagagcgg cacgaacgtc
      361 ggttcgtcgg caaaaacgtg cgtcgacgcg gcgaaggaga ggctgaacgc gagaagcgtt
      421 cggccgaggc gaggggtgaa gggcgatcgt ttcatgggga ttaagaagcg ttgaatgggt
      481 gcgctcgtcg ctgcgcggcg atgcgcggct cacggtgcat gcgatgattt cgccaatgga
      541 gcgcaaacca taagtgatcc gtgactctgc ctgcggcaga acgggtgacg ttcgatttgc
      601 aggtgcggca cagacgcgca ggcttctgga tgtcgaaaac gcacaaagaa attttgatgg
      661 aaggcaacga agcgatcgct cgaggtgatt tcgaaggctt cctggttcac tgcacggaaa
      721 acaccgtctg gaattttttg ggcgacaaaa cccttcgggg gaaagaggcg gtgcggcgct
      781 ggatgacgga agcctacaag gaaccgccca agttcaacgt tcgtgagctg atcgcagacg
      841 gtgagtttct cgcggcggtc ggagagatca cgctcaagga cgagaacggc aaagccgcgg
      901 aattttccta ctgcgacgtc tggcgcttcg aaggcagccg aatggctgag ctgaaagcgt
      961 tcgtcatcgc ccccaagacg aacgcataga agccgccaga ggcgggcctg cgccggttgt
     1021 cttgtttttc cgcgagtcgc aaacacgcgc gatcgcggtc tcaaaaacga agcgacgatg
     1081 gtcgccgcga cagcaaaaca gcaccggagg caggacctgc ttgcaggcaa tctcgcgcat
     1141 cgagtgcaga ggacgcatca tatcgcctgc aagcaggctc ctacaattac caggccgttc
     1201 gttcatcaag gtggagcccg acgtcctcgt cgggctcgat cacgttcgct cgcaaaacag
     1261 cccgacgaga acgtcgggct ccacctccgc gccgcgaaag cacgggccaa tggcttcacc
     1321 tcgtcaaaat caacccgcgc ctgcctcctc gatcaggcgc agggcgtcga cgttgaccca
     1381 gtcctgatgc ggaaacgttc gcgcccattg cgagggcgtg acctcgcggg cgatgtgcca
     1441 gcgcggcgtt tcctgccagt cgaggaactt gcgcacgttc gcccgcagag tcgtcgacgc
     1501 gccgcgtgcg aggagttcgc gcaccatcga tccgtcgcgg cgcccgtgcc cggaaaacac
     1561 gctgccgacg actgcgttaa acagcggcga atgtccgccg aagccttcgg cgtcgacgct
     1621 cgcgcgtgcg ttcacatccg cgccgtgctc gagcagcagt tcgaagatcg cacgttcgtc
     1681 gaagtcgatc gcgaggtgca ggagcgtgcc gcccgcgatc ggcgtgccgc acatgccgga
     1741 gcggccatcg ggcgcacagc cgagcgccgc aggataaatt tcctttgccg caaaccgccg
     1801 gtgcacgagg gttggttcgc gctgcaggaa ctcgcgcaac cggtccactt ggccgcgatg
     1861 aaacgccatc atcggcgtgt caggcaggtc gtagccttgg tcggcgaaga tcgccaggac
     1921 cgcgtgcttg ccagtgggat tccgcgcgta ggtttcgagc gcgagcgcga gcggggcgag
     1981 acggtcgccg cgggcatccg tgaagggtgc gcccaattcc acgagcaaac cgagtccggc
     2041 gacgtttaac gtttcgcagc agcccatgac gaggccgggc acgagcttcg cgccgtgagc
     2101 gtgaagccaa cgcgccgttt cgatcctgcc ctgaagcagc gcgcgatcga acgcgtgttg
     2161 gaaatcgcgc gcgcccagcg tggccagcgt ctgcacgagg tcgagccggc cgagattggc
     2221 ggcgtggctc atcggcgggc cccagtttcc gctccacacc ggaaggtgca gcaactccgg
     2281 atgaacgcgc agcatccgca ccacggcggc gcgttcgccc gcatgaatgg cgagacgcag
     2341 ttggtgggtc agcgccgtgg aagcgatctt gagcttcaac tgcgcccaat tcgcgaaacc
     2401 gtattcccgt gcgatgacga actgcgcgtc agccagcttg atcggcgccg acgcgagcgc
     2461 gggatcgttc gcggcgcgat accgcgggat ctgctgccgg atgcgttcga gcgcctcggg
     2521 gtcggcggcg cgccagcgtg cgagcagttc gcgcgcctcg cgtttgggaa cgtcgatatg
     2581 gggctgttca gggagcccgc gtgaaaccgt ggccataacc tttcctcctc agttgcgccc
     2641 gatgtccgca cgtgccgggc aggaagacag gttaagttga cctaaccgat caaccggtgg
     2701 gctgagccca tttccgcgga cggggtgctt ccggcaagca tcgtcaccgt gccgagcgga
     2761 cgagtttttt caaccgcgga ttttcgttcc gagtcatgac gacgtggcag ccgaacggcg
     2821 cggcgcggcg ctcggagcga tcgcactcaa ggatggtcac ggcaaagcca cggaatattc
     2881 ctcctgcgac gtctggcgct ttgaaaacgg ccgaatggcc gagctgacgg acgccgtcat
     2941 tgcgcttagc cgtattcatg cagaagacct cccgcgaatg acgcgaatga ccgcgaattt
     3001 cagagaggtg gaatcaaatg tggccgcttc aaggccagat caccgtttgg tgaaagatca
     3061 tttctcttcg tccgtattcg cggcgattgg cgtgattcgc ggccaactga atgatcgccg
     3121 ctaagtttaa cctgtagccg gcctcgatca tgcaacccac gaaaagtctc gtttccctcc
     3181 ggccagtggt cgcggacgat ctgccgatct tatttgctca ccagcgcgat cccgaatccg
     3241 tgcgactcgc cgcattcgtt tcgcgcgatc acgacgcttt catgctgcat tggaccaccc
     3301 gcattctcgg caaccccgcc gccgcgtgtc gcacggttct gttcgatgac aatgtggttg
     3361 gatatatcgg cgcgt
//
LOCUS       DASPLT010000387         1283 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_5032, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000387 DASPLT010000000
VERSION     DASPLT010000387.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1283)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1283)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1283
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5032"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1118
                     /locus_tag="VEA63_04010"
     CDS             <1..1118
                     /locus_tag="VEA63_04010"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF013319.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HYD83184.1"
                     /translation="RNVASLPFIHSHIAVMPDVHFGIGATVGSVIPTKGAIIPAAVGV
                     DIGCGMMAVRTSLTANDLPDSLSRLRGAIERNVPHGNGPNGNHRDTPSSVETTYRDSG
                     LDDRYRAIIDKHPKASAKAQTTQLATLGGGNHFIEVCLDEEDRLWVMLHSGSRGSGNA
                     IGKYFIERAKEELLRRELGYHVPDKDLAFFLEGEELFDDYVAAVGWAQDYARTNREVM
                     MERKLRALRDNLPKFKLEKHAVNCHHNYVARERHFGADVFVTRKGAVRAGEGELGIIP
                     GSMGAKSFIVRGKGNLDSFCTCSHGAGRAMSRTEAKRRFTLADHRAATEGVECRKDAG
                     VIDETPMAYKDIDAVIAAQADLIDVVHTLRQVVCVKG"
BASE COUNT          241 a          412 c          422 g          208 t
ORIGIN      
        1 tgcgcaacgt cgcttcgctg ccgttcatcc atagccacat cgcggtgatg ccggacgtac
       61 acttcggtat cggcgcaacc gtgggctccg tgatcccgac caagggcgcc attattccgg
      121 cggccgtggg cgtggatatc ggctgtggca tgatggctgt gcgcacctcg ctcacggcga
      181 acgatctgcc ggattcgttg tcgcgcttgc gcggcgcgat cgagcggaac gtgccgcacg
      241 gcaacggccc gaacggcaat caccgcgaca cgccctcttc ggtggagacc acgtaccgtg
      301 actccggcct cgatgaccgc taccgcgcga tcatcgacaa gcatccgaag gcgtcggcca
      361 aggcgcaaac gacgcagttg gcgacgctcg gcggcggcaa ccactttatc gaggtctgcc
      421 tcgacgaaga ggaccgcctt tgggtgatgc tgcactcggg ttcgcgcggc tccggcaacg
      481 cgatcgggaa gtactttatc gagcgggcga aggaagaact cctgcgccgc gagctcggct
      541 accacgtgcc ggacaaggat ctcgccttct tcctcgaagg cgaagagctg ttcgacgact
      601 acgtagcggc ggtcggctgg gcgcaagact acgcgcgcac caatcgcgag gtgatgatgg
      661 agcgcaagtt gcgtgcgctt cgcgacaacc tgccgaagtt caagctcgag aagcacgccg
      721 tgaactgcca ccacaattac gtggcgcgtg aacggcactt cggcgccgac gtgttcgtga
      781 cccgcaaggg cgcggtgcgg gcgggcgaag gcgaactcgg gatcatcccg ggatcgatgg
      841 gcgcaaagtc gttcatcgtg cgcggcaagg gcaacctcga ttcgttctgc acctgctcgc
      901 acggcgcagg ccgcgcgatg agccgcaccg aagccaagcg ccgcttcacg ctcgccgatc
      961 accgcgccgc caccgaaggc gtggaatgcc gcaaggacgc gggcgtgatc gatgaaacgc
     1021 cgatggccta caaggacatc gacgccgtca tcgccgcgca agccgacttg atcgacgtgg
     1081 tgcacacgct gcgccaggtc gtgtgcgtga aggggtgatc gcgacgtgtg agagcgatct
     1141 cacacgtcgt cactccagag ctgcgccgcc ggcgcaatga aattcagata gcccagcgcc
     1201 gcgatatcgt gtgcgtcagg gcgaaaaggg tgtgcgcggc gtccgcgcac cgcacaccca
     1261 cgctgcctgt tcgtctaatt ggc
//
LOCUS       DASPLT010000388         1642 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_148112, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000388 DASPLT010000000
VERSION     DASPLT010000388.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1642)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1642)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1642
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_148112"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            83..1438
                     /locus_tag="VEA63_04015"
     CDS             83..1438
                     /locus_tag="VEA63_04015"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WD40 repeat domain-containing protein"
                     /protein_id="HYD83185.1"
                     /translation="MPPAASIASRFTPSRLRAQTSTVAPVWSVSPGSTSYYINSVAVC
                     ADGSRTIAGTFLHTYGTAGRQPANGALAPSASDTGTFGTYCYNAAGQLQWKDEFNGWQ
                     GVYWVAISTSGAYAASGGWFSGSPNYAGFVRAYDASTGKMLLNYATPSRVNQVVLSAD
                     GTWLLAATTTLLLFKLVNGAYQRTGQFDPGGTNNTVITADMSASGQQIVCGDLNGSLY
                     AIANSSGQPTLQKAWPLPQKGYSHSVRITPSGSSFAAGGASGYFYLFDVGDFLVAGKP
                     AMTQQVAGGGSVYGVAIADDASAFVGISNLTPGSNVGGLVSYVIRRGGLAGVLWKFQT
                     DRNPNCASLNQANGMVAIADGHPDGSPGDFYLLDASTGALRWKYTTGNMSWPIQIAAN
                     GSAIVGGSDDSQVYYFTPQGGAGAKLNGKTVRRRRAKAKVRKPKASAYGWARRERRAA
                     G"
BASE COUNT          284 a          469 c          585 g          304 t
ORIGIN      
        1 ttgccgcgga agcaatcggt tgtctccgcg aatcgttcaa gtgctttggc aacgtgccgt
       61 tatacggcga atgcaaacag caatgccccc cgctgcttcg atcgcctcgc gtttcacgcc
      121 gtcgcgcctg cgcgcgcaga cttcgacggt cgctcccgtg tggagcgtgt cgccgggtag
      181 caccagctac tacatcaact cggtggccgt gtgcgccgat ggcagccgca cgatcgcggg
      241 cacgtttctc catacgtatg gcacggcggg ccggcagccg gccaacggcg cgcttgcacc
      301 gtcggcgtcg gacacgggga ccttcggcac gtattgttac aacgccgcgg gacaactcca
      361 gtggaaggat gagttcaacg gctggcaggg cgtttactgg gtcgcgattt cgacgagcgg
      421 agcttacgcg gcgtcgggtg gatggttttc gggcagccca aattacgcgg gcttcgtgcg
      481 cgcttacgac gcgagcaccg gaaagatgtt gttgaactat gcgacgccga gccgcgtgaa
      541 ccaagtggtg ctttcggccg acggcacgtg gctgctcgcg gcgacgacga cgttgctgtt
      601 gttcaagctc gtgaacggcg cgtatcagcg caccgggcag ttcgatcccg gcggcacgaa
      661 caacacggtc atcaccgcgg acatgtcggc gagcggtcag cagatcgtgt gcggcgatct
      721 caacggcagc ctgtatgcga tcgcgaattc gagcggccag ccaacactgc agaaagcgtg
      781 gccgctgccg cagaaaggct acagtcactc ggtgcgaatc acgccgagcg gatcctcgtt
      841 cgcggcgggt ggggcgagcg gttattttta tctgttcgat gtcggcgatt ttctcgtcgc
      901 gggaaaaccg gcgatgacgc agcaggtggc gggcggaggc agcgtctacg gcgtggcgat
      961 cgcggatgac gccagcgcgt ttgtcggaat ctcgaatctc acgcccggca gcaacgtcgg
     1021 cggactcgtt tcgtatgtga ttcgccgcgg cggtctcgcg ggcgtgctct ggaaattcca
     1081 gacggatcgc aatccgaact gcgcgtcgct caatcaggcg aacgggatgg ttgcgattgc
     1141 ggatgggcat ccggatggtt cgccgggcga cttttatctg ctcgatgcat cgacgggcgc
     1201 gctgcgctgg aaatacacga cgggcaatat gagctggccg attcagatcg cggcgaacgg
     1261 ctcggcgatc gtgggcggca gcgacgattc gcaggtttat tatttcacgc cgcaaggcgg
     1321 cgccggcgcg aaactcaacg gcaaaaccgt gcggcgtcgc agagcgaaag cgaaggtgcg
     1381 caagccgaag gcgagcgctt acggatgggc ccggcgcgaa cggcgcgcag cgggctgagc
     1441 ggacgggttt gggcagcgtg aaaagccgtg ggctggcgcg tccacggcga gttgatcgac
     1501 gacggacgcg ggttgcgtgc ggggagttgc gggcggttgc gctcggggtt gcgtcgcacg
     1561 cgaaatcggg cagaggtttt tcgcatgacg acttcgcgtt gggtgttcgc ggccggctgc
     1621 gggctggctg ctttatcgca gt
//
LOCUS       DASPLT010000389         2495 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_65812, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000389 DASPLT010000000
VERSION     DASPLT010000389.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 2495)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2495)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2495
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_65812"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1295
                     /locus_tag="VEA63_04020"
     CDS             <1..1295
                     /locus_tag="VEA63_04020"
                     /inference="COORDINATES: protein motif:HMM:NF000658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YdiU family protein"
                     /protein_id="HYD83186.1"
                     /translation="AGAEIFAGNRLPPGAAPLAQAYAGHQYGHFTTLGDGRAILLGEQ
                     ITPRGERFDIQLKGSGPTPYSRRGDGRAALGPMLREYIVSEAMHALGIPTTRSLAVAA
                     TGEEVLRERRLPGAVLTRVAASHIRVGTFEWAAATRDEAAFRALVDYTLQRHYPQLRG
                     AANPARALLDAVIERQAALIAQWMLVGFVHGVMNTDNMALSGETIDYGPCAFMDAYDP
                     ATVFSSIDRHGRYAYGNQPAIAQWNLTRLAESLLPLLHPQEDNAIALAEESLHMFPDR
                     FQHHWLAGMRRKLGLFNEEPDDVALIKTLLEWMRRTKADFTNTFATLANSDAPDAAST
                     SDAEFRRWRESWHARLRHQPQSAEESAQLRRESNPAFIPRNHLVEAALAAAEQGDLSV
                     MHRLLDVLASPYDHTRVAPDYTCPAPDGSADYQTYCGT"
     gene            1625..>2495
                     /locus_tag="VEA63_04025"
     CDS             1625..>2495
                     /locus_tag="VEA63_04025"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HYD83187.1"
                     /translation="METHPASAFIPAERILMGPGPSPVPQRVLRALAAPTLGHLDPQY
                     LAIMDRTCAMLREVFRTKNALTFPVSGTGMAGMECIAVNLLEPGDEAIVGVNGVFGTR
                     MKDVMERCGAKVHAVEAAWGDVITLDQVQAALDAHPKAKLVAIVHAETSTGVHQPLEG
                     FADLVHARGALLVVDAVTSLGGHELRVDDWGIDAIYSGTQKCLSCPPGLSPVSFSERA
                     LAAMDARKTKVQSWYLDVSMLRKYYTGGGGGRVYHHTAPINMTYALHEALTIVLEEGL
                     DARIERHAQAHRRL"
BASE COUNT          443 a          870 c          748 g          434 t
ORIGIN      
        1 acgcgggcgc ggagattttc gccggcaacc gcctgccgcc cggcgccgcg ccgctcgcgc
       61 aggcgtatgc aggacaccaa tacgggcatt tcaccacgct cggcgacggt cgcgcgatct
      121 tgctcggcga acagatcaca ccgcgcggcg aacgcttcga catccaactc aaaggttcgg
      181 ggccaacgcc gtattcacga cgcggcgacg ggcgcgccgc gctcggcccg atgctgcgcg
      241 aatacatcgt cagcgaagcg atgcacgcgc tcgggattcc aaccacccgc agcctcgcgg
      301 tcgccgccac cggtgaagag gtgctgcgcg agcgccggct gcccggcgcc gtgctgacgc
      361 gcgtcgctgc cagtcatatt cgtgtcggca cgttcgagtg ggccgcggcg acgcgcgacg
      421 aagcggcgtt ccgagcgctc gtcgattaca cgttgcaacg ccactacccg cagctgcgcg
      481 gcgccgccaa ccccgcgcgc gcgttgctcg acgccgtgat cgaacgacaa gccgcgttga
      541 tcgcacagtg gatgctcgtc ggcttcgtcc acggcgtgat gaacaccgac aacatggccc
      601 tttccggcga gacgatcgac tacggtccgt gcgccttcat ggacgcgtat gatcctgcga
      661 ctgtcttcag ctcgatcgat cgccacggcc gctacgccta cggcaaccag cccgcgattg
      721 cgcaatggaa tctcacgcgc ctcgccgaat cgctgctgcc gctgttgcac ccgcaagagg
      781 acaacgcgat cgcgctcgcc gaggagtcgc tgcacatgtt tcccgaccgc tttcaacatc
      841 actggctcgc cggtatgcga cgcaaactcg gcttgttcaa tgaggagccc gacgacgttg
      901 cgctgatcaa aacgctgctc gagtggatgc gccgcaccaa ggccgacttc acgaacacgt
      961 tcgccacgct cgcgaactct gatgcgcccg acgctgcctc gacatcggac gcagagtttc
     1021 gccgctggcg cgaaagctgg cacgcccgcc tccgccatca gccgcaatcg gccgaggagt
     1081 cggcccaact tcgccgcgag agcaacccgg ccttcatccc acgcaatcat cttgttgaag
     1141 ccgcgctcgc ggcggcggag caaggcgact tgtcggtgat gcaccgcctg ctcgacgtgc
     1201 tcgcctcgcc ctacgatcac acgcgcgtcg ctcccgacta cacctgcccc gccccggacg
     1261 gcagcgcgga ctaccaaacg tattgcggca cctgagccgt tcacgattcg cagtgtcggc
     1321 tcagcaaaag tggaacccac gtcctgtcgg gtttgatttg attttcgcgt gaacccgcat
     1381 tcgtcgcacg cagatgcggg gtgcgcgcag tctgttgtag cgaaacgcgt gagcgtttcg
     1441 tccgggcgag agacgaaacg ctcacgcgtc tcgctacagg tgtgaatttt ccgggttaag
     1501 cgcgcactcc agcccgacga ggacgtcggg ctccacgatc ggctcgcgag cgttcgcttg
     1561 ctccacggcc gcgcaactcc gcgctcgtca ccgcgcgttt cgcggttcaa tcgtttttta
     1621 cctcatggaa actcaccccg cttccgcttt cattccggcc gaacgcattc tcatgggacc
     1681 cggtcccagc cccgtgcctc aacgcgtgct ccgcgccctc gccgctccga cgctcggtca
     1741 tctcgatccg caatacctcg ccatcatgga tcgcacgtgc gcgatgttgc gcgaggtgtt
     1801 tcgcaccaag aatgcgctca cgtttcctgt gagcggcacc ggcatggccg gcatggagtg
     1861 catcgcggtc aacttgctgg agccgggcga cgaagccatc gtcggcgtca acggcgtgtt
     1921 cggcacgcgc atgaaagacg tcatggaacg ctgcggcgca aaagtgcacg ccgtcgaagc
     1981 cgcttggggt gacgtcatca cgctcgatca agtgcaagcc gcactcgacg cacatccgaa
     2041 agcgaaactc gtcgcgatcg ttcatgcgga gacgagcacc ggcgtgcatc agccgctcga
     2101 aggttttgcg gatctcgtcc atgcgcgcgg cgcgttgctc gtcgtcgatg cggtcacgag
     2161 cctcggcgga cacgagttgc gcgtcgacga ttggggcatc gacgcgatct acagcggcac
     2221 gcaaaaatgt ctctcctgcc cacccggcct ctcgcccgtc agcttcagcg aacgcgcact
     2281 cgccgcgatg gacgcgcgaa agaccaaggt gcagtcgtgg tatctcgacg tctcgatgct
     2341 ccgcaagtat tacaccggtg gcggcggcgg acgcgtttac catcacactg cgccgatcaa
     2401 catgacgtat gcgttgcacg aggcgttgac gatcgtcctt gaagaaggac tcgacgcccg
     2461 tatcgagcgt cacgcgcaag cacaccgccg gctcc
//
LOCUS       DASPLT010000390         1090 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_169040, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000390 DASPLT010000000
VERSION     DASPLT010000390.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1090)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1090)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1090
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_169040"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(46..789)
                     /locus_tag="VEA63_04030"
     CDS             complement(46..789)
                     /locus_tag="VEA63_04030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD83188.1"
                     /translation="MNMDNSKLGGGGKPVDAANRNSGDRASRRGLAGPLLRATALVFA
                     VSVLVSVPFMIFGEALFVPVFEAVRHSTFWLVLLAIALLAADALLPVPSAWVVIFLAQ
                     QSGALAGMIGGTLGLSAGVLVAGWIGRAAVGRAAPKFFPEAELARLRDSMQRHTIVTL
                     ACMRSVPVLAETSVLIAAGAGVPLRRIFWATLPPNVAISVVYSFAADESFLTACLAFL
                     ATVGVSYAAWRVHAWLAAREKVMNGGASS"
     gene            complement(811..>1090)
                     /locus_tag="VEA63_04035"
     CDS             complement(811..>1090)
                     /locus_tag="VEA63_04035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD83189.1"
                     /translation="NLLCVAGVPDLVQNPVERFVPAIVLQRLEATMPRKELVQVLTWI
                     ALHPNEGTVITDLSALQLDGAGSEYHVRERSNWYAMKFVAKLTGRATD"
BASE COUNT          226 a          437 c          273 g          154 t
ORIGIN      
        1 tatcgaactc tctgaaccgc tgtagctctg aagacggccg cgccgtcagc tcgacgcccc
       61 gccgttcatc accttctcgc gcgccgccag ccaggcgtgc acacgccacg cggcatacga
      121 cacgccgacc gtcgccaaaa acgcgaggca cgccgtcaaa aaggattcgt ccgcggcaaa
      181 cgaatacacc accgagatcg cgacgttcgg cggcagcgtt gcccagaaaa tacgccgcaa
      241 cggcacgccc gcgcccgccg cgatcaacac cgacgtctcc gccaacaccg gcacgctgcg
      301 catgcacgcg agcgtgacga tcgtgtgccg ctgcatcgag tcgcgcagcc gcgccagctc
      361 cgcctcggga aaaaacttcg gcgccgcccg cccgactgcc gcccgcccga tccaacccgc
      421 gacgagcact ccggccgaca gccccaacgt cccaccaatc atgcccgcca aggcgccgct
      481 ctgctgtgcc agaaaaatca ccacccacgc cgacggcacc ggcagcaacg catccgccgc
      541 cagcagcgcg atcgccagca gcaccaacca gaacgtgctg tgccgcaccg cctcgaacac
      601 cggcacaaac aacgcctcgc cgaaaatcat gaacggcacg ctcacgagca cgctcaccgc
      661 aaacaccagc gccgtcgcgc gcaacagcgg ccccgccaac ccgcgtcgcg atgcgcggtc
      721 gccgctgttt cggttggccg cgtcaaccgg tttccctccg ccgccgagct ttgaattatc
      781 catgttcatc cgcgtcgatc cgtgaagttc tcagtccgtt gcccgacccg taagtttcgc
      841 cacaaatttc atcgcatacc agttcgagcg ctcccgcacg tgatactccg atcccgctcc
      901 gtccaactgc agcgccgata aatccgtgat caccgtgcct tcgtttggat gcagcgcgat
      961 ccaggtcaac acctgcacca gttccttccg cggcatcgtc gcttccaatc gctgcaacac
     1021 gatcgccggc acgaaacgct ccaccggatt ctggaccaga tccggcacgc ccgccacgca
     1081 gagcaaattc
//
LOCUS       DASPLT010000391         1554 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_270961, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000391 DASPLT010000000
VERSION     DASPLT010000391.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1554)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1554)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1554
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_270961"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..239)
                     /locus_tag="VEA63_04040"
     CDS             complement(<1..239)
                     /locus_tag="VEA63_04040"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HYD83190.1"
                     /translation="MKSFCLTLALLLLAIPPALCGAASGEEKKLNVLFLVADDLNCDL
                     GAYGATYAHTPNLDALAARSVRFERAYTQFPHCSP"
     gene            complement(236..586)
                     /locus_tag="VEA63_04045"
     CDS             complement(236..586)
                     /locus_tag="VEA63_04045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD83191.1"
                     /translation="MSLLPVSRNPSATVRDYVFAEQNWHNFTAHVRMVRKGDFVYLRK
                     AASERSLPDASDTFYTPSAETGDTVPAQPTASNIVLATGERIALRRGNTPGAAAGAAR
                     INAPGPIRANDILQ"
     gene            complement(802..>1554)
                     /locus_tag="VEA63_04050"
     CDS             complement(802..>1554)
                     /locus_tag="VEA63_04050"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HYD83192.1"
                     /translation="TYGDFVRQLDADVGQLLAALDELGLAENTLVVFTSDNGFAPAVD
                     VPLHARIGHDPSAGRRGFKSDLYEGGHRVPFFARWPGRVSPGSRNDALVGQLDLFATC
                     AELAGVPLPAAAAEDSVSFLAALRGEPVTRTTLVNHSGEGRFAIREGRWKLLLWPGSG
                     GWSSPTPNPSRWLKVPATDLATLPPYQLYNLTSDPAEQRNVAAEHPEIVQRLGRLLSE
                     QIERGRSTPGAAVAEAASADWPELSWRADFAP"
BASE COUNT          293 a          483 c          514 g          264 t
ORIGIN      
        1 ctcggcgaac aatgcggaaa ctgcgtatac gcacgctcga accgcacgct gcgcgccgcc
       61 aaggcgtcaa ggttcggcgt gtgcgcgtag gtcgcgccgt aggcgccgag atcgcagttg
      121 agatcgtctg cgacgaggaa aagcacattg agcttttttt cttcgcccga cgcggccccg
      181 cacagcgcag gcggaatcgc gagcaataac aaagccaagg taaggcagaa ggatttcatt
      241 ggagaatgtc gttcgcccgg atcgggccgg gggcattgat ccgcgcggcg ccggcggccg
      301 ctccgggagt atttccgcgt cggagcgcga tacgttcgcc cgtggcgagc acgatattcg
      361 aggcggtcgg ctgcgccggc acggtgtcgc cagtctccgc gctcggggtg taaaaggtat
      421 ccgatgcatc cggcagcgac cgctcggacg ccgccttacg caggtagaca aaatcgccct
      481 ttcgcaccat tcgcacgtgt gcagtaaagt tatgccagtt ctgctcggcg aacacatagt
      541 cgcgcaccgt cgcactcgga ttgcgcgaga ccggcagcaa gctcacgcct tggaagctct
      601 cgccgtcata gttgagtttt gcgagcccaa ggccatgctc gccagcgatc gacgcgggtg
      661 aagaacgttc cgttcgatgc gagacgggtc gagcgcggcg tattcaaata ttcgcgaccc
      721 tccatctgcc cagatcgtcg gcgatgtgct gatcggtcat gatgaacatg atgttcgccg
      781 cagcgccggc cgccaccgcg atcagggagc gaaatcggcg cgccaggaaa gttccggcca
      841 gtcggcagac gccgcttccg caaccgccgc gcctggcgtg ctgcggccgc gttcgatttg
      901 ttcgctgagc aaacgaccga gacgttggac tatctcgggg tgttccgccg cgacgttgcg
      961 ttgttcggcc ggatcgctcg tcaaattgta gagttgataa ggcggaagcg tggcgagatc
     1021 cgttgcggga accttgagcc agcggctggg gtttggcgtc ggtgaactcc atccgccgga
     1081 accgggccag agcaggagtt tccaacgtcc ttcgcgaatc gcgaatcggc cttcgccaga
     1141 gtggttcacc aacgtcgtgc gcgtgaccgg ctcgcctcga agcgccgcga gaaaactgac
     1201 gctgtcctcc gccgcggccg ccggaagcgg cacgcccgcg agttcggcgc acgtcgcgaa
     1261 aaggtcgagc tgaccgacca gcgcgtcgtt gcgcgagccc ggcgaaacgc gaccgggcca
     1321 gcgggcgaaa aacggcacgc gatgcccgcc ttcatacagg tccgacttga aaccgcgccg
     1381 gcccgcgctc gggtcgtgac cgatgcgcgc gtgcagcggc acgtcgaccg cgggcgcaaa
     1441 gccattatcg gacgtgaaca cgaccaaggt gttttccgcg aggccgagtt cgtcgagcgc
     1501 agcgagaagt tggccgacat ccgcatcgag ttggcggacg aaatcgccgt aagt
//
LOCUS       DASPLT010000392         2284 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_5121, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000392 DASPLT010000000
VERSION     DASPLT010000392.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 2284)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2284)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2284
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5121"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..194)
                     /locus_tag="VEA63_04055"
     CDS             complement(<1..194)
                     /locus_tag="VEA63_04055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017954035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD83193.1"
                     /translation="MPVAQPHSAARIRIALGAVTFAALLLELSLTRLFSVVLFYHFAF
                     LAISVALLGLGAGGVLAFLYR"
     gene            complement(199..576)
                     /gene="acpS"
                     /locus_tag="VEA63_04060"
     CDS             complement(199..576)
                     /gene="acpS"
                     /locus_tag="VEA63_04060"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HYD83194.1"
                     /translation="MIVGIGLDIAEVDRVEAAIARHGERFLNRVYTPAERSYCESKVN
                     RWERFAGRFAAKEAAMKAIGTGWKRGVAWREFEVRRAPSGQPIIEFHGRAGEIARSLG
                     VKRALVTISHTAQHAIAQVLLEG"
     gene            complement(974..2131)
                     /locus_tag="VEA63_04065"
     CDS             complement(974..2131)
                     /locus_tag="VEA63_04065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD83195.1"
                     /translation="MSYSYSNPSRVVICLLLASLLPVPVAGQYSARKPERKSPRGVGV
                     LQISPAGVARLLPVVIYEGGKYYDATFYRSRPVPMSLVSDTLYEAQHAGMPAGTFTVR
                     GAQRHGNAWVGVGVWKPTNAPADPAAKPSEAKAADAKPALKDDGDPERPVLRRPASAS
                     SAAPAPSPSPASGTPAPEDPDRPVLKRGGAPQVKPDSPEDAAKPIVVASRMVAVSDPE
                     PATARPLEYMADDAEKAKLAAAMQELGLAELRRNAAALRIAPPARSAKLTDVRVHFYD
                     IDYSNNPQLVLNGRYLPAGAARPLLVALVARTDYNGQMTRVFFRLGDPSNLADRPELT
                     FIDVVDLEGDGRAELLFERRTDEGTGFVAYRLVGQSMSEVFATAARTSRAQ"
     gene            2130..2258
                     /locus_tag="VEA63_04070"
     CDS             2130..2258
                     /locus_tag="VEA63_04070"
                     /inference="COORDINATES: protein motif:HMM:NF012787.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HYD83196.1"
                     /translation="MEVNDKAPDFTLLDENGNTVKLSQFRGEHVVLFFYPKANTPG"
BASE COUNT          378 a          701 c          801 g          404 t
ORIGIN      
        1 cggtacagga acgcgagcac gccgccggcc ccgagtccga gcagggcgac cgagatcgcg
       61 agaaacgcga agtggtagaa gagcacgacc gaaaacaggc gcgtgagcga gagttccagc
      121 aggagggcgg cgaacgttac cgcgccgagg gcgatgcgga tgcgtgcggc ggaatggggc
      181 tgcgcgaccg gcaatcggtc agccctcgag cagcacctgg gcgatggcgt gctgcgctgt
      241 gtgagaaatc gttacgagtg cgcgcttgac tccgagcgag cgggcgatct cgccggcgcg
      301 tccgtggaac tcgatgatgg gctgacccga aggcgcacgg cgaacctcga actcgcgcca
      361 cgctacgccg cgcttccacc cggtgccgat ggccttcatg gcggcttcct tggcggcgaa
      421 gcgtccggcg aagcgttccc aacggttgac cttggactcg caatagctgc gctcggcggg
      481 cgtatagacg cggttgagga agcgctcgcc atggcgcgcg atggcggctt ctacgcggtc
      541 cacttctgcg atatcgagtc cgattccgac aatcatgtgc gttctggccc agacatcggg
      601 acaacgtagc ctacacgagg gaaggtgaag tgaccaacgc ggggttggtg agtgtcgtcc
      661 ctgcgggact cgatggttgg tgcgatcgct ccccagcact gacgtgctgg gctattgttg
      721 tgccgcgcct tcggcgctcc cagcttgaca gagctctgcg atttgaagaa ctaccccacg
      781 ttagctccgc cttcggctcc gcggaacgtg gggcacccgc attgtcgcca tttgatgcac
      841 taccccacgt tagctccggc ttcgcctccg cggaacgtgg ggcacccgtc cgattttgag
      901 cactacccca cgttagctcc ggcttcgcct ccgcgaacgt gggccaccct ctgaacgtca
      961 ggcacctcag ccactactgg gctctcgatg tgcgcgccgc tgtcgcgaag acctcgctca
     1021 tcgactggcc cacgaggcgg taggcgacga acccagtgcc ttcgtccgtg cggcgctcga
     1081 aaagcagctc agcccttccg tctccctcga ggtccactac atcgatgaat gtgagttccg
     1141 ggcggtcggc gaggttcgat ggatctccca ggcggaagaa cacccgcgtc atctggccgt
     1201 tgtagtccgt gcgggcgacc agcgcgacca gcagcggacg cgcggccccg gcaggcaggt
     1261 agcgcccgtt gagcaccagt tgcgggttgt tgctgtagtc gatgtcgtag aagtgcacgc
     1321 gcacgtcggt cagcttggcg ctcctggcgg gcggggcgat ccgaagcgcg gcggcgttgc
     1381 ggcgaagttc ggccaacccc agttcctgca tggcggctgc gagctttgct ttttctgcgt
     1441 cgtcggccat gtattcgagc ggacgcgcgg tcgcaggctc ggggtcggag acggccacca
     1501 tgcgactggc aacgacgatc ggctttgcag cgtcttccgg agagtcgggc ttgacctgtg
     1561 gcgcgcctcc gcgcttcaac accgggcggt cgggatcttc aggtgcgggc gtgccggaag
     1621 caggcgaggg cgagggtgcc ggggcggcgc tggaggccga ggccggacgc cggaggacgg
     1681 gacgttcggg atcgccgtcg tccttaagcg cgggcttggc gtcggcggcc ttcgcttcgc
     1741 tgggcttggc cgccgggtcg gcgggcgcgt tggtgggctt ccatacgccg actccgaccc
     1801 aggcgttccc gtggcgctgc gctccccgga ccgtgaaggt tccggcaggc atgccggcgt
     1861 gctgcgcttc gtaaagggtg tcgctgacga gcgacatggg caccggcctg gagcggtaga
     1921 acgtcgcgtc gtagtactta ccgccttcat agatgacgac cggcagcagg cgcgccactc
     1981 cggcagggct gatctggaga actccgacgc cgcgcggaga cttgcgttcc ggcttgcgcg
     2041 cgctgtattg gcccgccaca ggaaccggga gcagagatgc gagcaggagg cagatgacga
     2101 ctcgggacgg gttggagtat gaatagctca tggaagtgaa cgacaaagca cccgatttta
     2161 ctttgttgga tgagaacggc aacacggtga agctctcgca gttccgggga gagcacgtag
     2221 tgctgttctt ttatcctaag gcgaacactc ccggttgaac gatcgaggcg tgcgggttcc
     2281 gcga
//
LOCUS       DASPLT010000393         1498 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_209889, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000393 DASPLT010000000
VERSION     DASPLT010000393.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1498)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1498)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1498
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_209889"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1328)
                     /locus_tag="VEA63_04075"
     CDS             complement(<1..1328)
                     /locus_tag="VEA63_04075"
                     /inference="COORDINATES: protein motif:HMM:NF024240.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HYD83197.1"
                     /translation="MTFDNVGIAPWSVATELNPNLAQPMKHANPSTQAEVAPADVARH
                     PCHILPTLLAGAFIRARRKQRERFVSNRCASWLLLLALPCAGADAAAVSVREAEIVIY
                     GGTAGGIAAGIQAKRMGRDVVVVEPSAHIGGLTTGGLGQTDIGAKHVIGGIAREFYRG
                     IRSHYQQPEAWKWQRREDYRDGGQTRTDSNEDTMWTFEPTAAKAVLNAMIENAGLTVI
                     RNERLDRAHGVTKNGARIAAIRFESGLEVRGRMFIDATYEGDLLAAAGVRYRVGRESN
                     AEYGETLNGVQTDRYARTLAHGLSLNAGNHQLASRVDPYRSPGDASSGLLPGIRSGGP
                     GIEGAGDAGVQAYCYRVCLTNHADNRVPFARPADYDELNYELLLRHLEADPNARIWIN
                     SDMPNRKTDTNNGGGFSGDFIGGNHRYPEAGYAERAAIAAAHRSYQQGLY"
BASE COUNT          255 a          498 c          452 g          293 t
ORIGIN      
        1 caatacagcc cttgctgata agagcgatgc gccgcggcga tcgcggcgcg ttccgcgtag
       61 cccgcttcgg ggtagcgatg gtttccgccg atgaagtcgc ccgaaaatcc gcctccattg
      121 ttcgtatccg ttttccggtt cggcatgtcg gagttgatcc agatgcgcgc gttgggatcg
      181 gcctcgagat ggcgcaggag caattcgtag ttcaactcgt cgtaatctgc agggcgggcg
      241 aacggcaccc ggttgtcggc atgattcgtc aggcataccc ggtaacaata cgcctgcacg
      301 ccagcgtcac cggcgccttc gatccccggc ccgcccgagc gaatgccagg cagcagcccg
      361 ctcgatgcgt ctccgggcga acgatacgga tcgacccgcg acgcgagctg gtggttgcct
      421 gcattcaacg acaggccgtg cgccagcgtg cgcgcatagc ggtcggtctg caccccgttg
      481 agcgtctcgc catactcggc gttgctctcg cgtccgacgc gatatcgaac gcccgcggcc
      541 gcgagcaaat cgccttcgta ggtggcgtcg atgaacatgc ggccccgcac ctcgaggccg
      601 ctctcgaaac gaatcgcggc gatgcgcgcg ccgtttttcg tcacgccgtg tgcacgatcc
      661 agccgctcgt tgcgaatcac ggtcaatccg gcgttttcga tcatcgcgtt caacaccgcc
      721 ttcgccgccg ttggttcgaa ggtccacatc gtgtcctcgt tagagtctgt gcgcgtttgt
      781 ccgccgtcac ggtaatcctc gcggcgctgc catttccatg cctccggctg ctggtaatga
      841 cttcggattc cacggtaaaa ttcgcgcgcg atcccgccga tcacgtgctt cgccccgatg
      901 tcggtctgac cgagaccgcc cgtcgtgagg ccaccgatat gcgccgacgg ctcgaccacc
      961 acgacgtccc ttcccatgcg tttcgcctgg atcccggccg caatacctcc cgccgtgccg
     1021 ccgtagatga caatctcagc ttcgcgaacg gacaccgccg cggcatccgc tccggcgcaa
     1081 ggcaaggcga gaagcaagag ccagctggcg caccgattcg aaacgaagcg ctcccgttgt
     1141 ttgcgccgcg cgcgaatgaa cgccccagcg agcaaggttg gcaggatgtg gcaggggtgg
     1201 cgtgcgacat cggccggcgc cacttcagcc tgcgtcgagg gatttgcgtg tttcatcggt
     1261 tgggcaaggt tggggttcaa ttcggtcgcc accgaccacg gcgcgattcc gacgttatcg
     1321 aaagtcacgg tagccgaggg ttgaccttcg cagggttatg ataatcgcgc cggtgcgcca
     1381 gcccgcgttc aaggcacgtc gtcgcgcata aattcaatat gaacgccaaa ttcccttccg
     1441 gccgaaccgg cccagacatc cgccttttag cccgtgtttc gtgacaatct cggcgggc
//
LOCUS       DASPLT010000394         2028 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_44613, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000394 DASPLT010000000
VERSION     DASPLT010000394.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 2028)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2028)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2028
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_44613"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2028
                     /locus_tag="VEA63_04080"
     CDS             <1..>2028
                     /locus_tag="VEA63_04080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD83198.1"
                     /translation="FQVYYDYDSYNLERFDFARGPNSILFGSGGMGGTANALYKRART
                     DKPFVTVQTAVGSWSNYRATLDVNQPLSDRLAIRFNAMWDESETWRDKEYFDKMGATL
                     ALMFKPWKGGELRVTGDIGEYHRNASLTTLGDQVSGWDGVTVFNGPATANNAKGVSVY
                     GANTYTFSPSTANGAIVNYAGYGLTMGGNSNAAVPIGGALVVGPSAGYAANPILDAVF
                     RPANAFDKAIAGSEFFIPGREFTTTHDGDTWNSDFHNVLVSFDQQVGDHLFLGISGSD
                     AAGLNRTDYTIVRGLNNVFIDINQTLPGGGQNPNFLKPYSESPRDYDEVDRTGRNYRL
                     SAALVFDETRFGSFRLNLEAGDSRFTSSRAKYRQQVKDPAVLPRNWITGVVRYRYYWG
                     ESQAFDPGIGTFNFGNKDFIDPVGGNRSMEVGMLLDSGRPGETIITTDDYEYYQAAFS
                     AKLFSERLHLLAAVRRDDFSSRTDHMLLRGDLPDNWNGLDIIYRPRPPEDYYSLPTTR
                     PRVGNSVNNPRQPGFENQRYQDDYSVPASDGAIDTMSMGAVYHVTNWLSVFGNFAETW
                     VPPTKDLRIDFSRFDPVTSEGWDAGIRLSLLNDRISLSASRFESEQVNLAVGTGTGTG
                     GLSTSLPNAFNAIASANVLGDTTPDGMNNRGMSLVPSVYSDTAARRSEGYE"
BASE COUNT          388 a          578 c          682 g          380 t
ORIGIN      
        1 cttccaagtc tactacgatt acgacagcta caatctcgag cgcttcgatt tcgcgcgcgg
       61 accgaattcg attctctttg gcagcggcgg catgggtggc acggcgaacg cgctctacaa
      121 gcgggcgcgg acggacaagc cgttcgtcac ggtgcagacg gcggtcggct cgtggtcgaa
      181 ctaccgcgcg acgctcgatg tgaaccagcc gctgtcagac cggctcgcga ttcggttcaa
      241 cgcgatgtgg gacgagagcg agacgtggcg cgacaaggag tatttcgaca agatgggcgc
      301 gacgctcgcg ctgatgttca aaccgtggaa aggcggcgaa ctgcgcgtga ccggcgacat
      361 tggcgagtat catcgcaacg cgtcgcttac cacgctcggc gatcaggttt cgggctggga
      421 tggcgtgacg gtgttcaacg ggccggcgac ggcgaacaac gccaaaggtg tctcggtgta
      481 tggcgcgaac acctacacgt tttcgccgag cacggcgaac ggtgcgattg tgaattatgc
      541 gggctacgga ttgacgatgg gcggaaactc gaacgcggcg gtgccgatcg gcggcgcgct
      601 ggttgtgggg ccgagcgcgg gctatgcggc gaatccgatt ctcgacgcgg tgtttcgtcc
      661 ggcgaacgcg ttcgacaaag cgatcgcggg gtcggagttc tttatcccgg gacgcgagtt
      721 cacgacgacg cacgacggcg acacgtggaa ttcggatttt cacaacgtgc tggtttcatt
      781 cgaccagcag gtgggcgatc accttttcct cggcatttcg ggcagtgacg cggccggctt
      841 gaaccgcacc gattacacga tcgtgcgcgg gttgaacaat gtgttcatcg acatcaatca
      901 gacgctgccg ggcggagggc agaatccgaa tttcctaaag ccttactcgg agtcgccgcg
      961 cgattacgat gaagtcgacc gcacggggcg caactatcgg ttgagcgcgg cgttggtgtt
     1021 cgacgagacg cgcttcggca gcttccggct gaatctcgaa gcgggcgaca gtcgcttcac
     1081 gtcgagtcgc gcgaagtatc gccagcaggt gaaggatccg gcggtgctgc cgcgaaactg
     1141 gatcacgggc gtcgttcgct accgctatta ttggggcgag tcgcaggcgt tcgatcctgg
     1201 gatcgggacg tttaatttcg gcaacaagga cttcatcgat ccggtgggcg gcaatcgttc
     1261 gatggaagtc gggatgctgc tcgattccgg ccggccgggc gagacgatca tcacgaccga
     1321 cgattacgaa tactaccagg cggcgttcag cgcgaagttg ttcagcgagc gcctgcatct
     1381 gctcgcggcg gtgcgacgcg acgatttctc gagtcgcacg gatcacatgc tgttgcgcgg
     1441 cgatttgccg gacaactgga acggcctgga cattatctat cggccgcgcc cgcccgagga
     1501 ttattactcg ctgccgacga cgcgtccgcg cgttggcaac agcgtgaaca atccgcggca
     1561 gccgggattc gagaaccagc gttatcagga tgattactcg gtgcctgcgt cggacggcgc
     1621 gatcgacacg atgtcgatgg gcgcggttta ccacgtgacc aactggctga gcgtcttcgg
     1681 caatttcgcc gagacgtggg tgccgccgac caaggatctg cgcatcgatt tcagccgctt
     1741 cgacccggtg acgtcggaag gttgggacgc gggcatccga ctgagtttgc tcaacgaccg
     1801 aatcagcctg agcgcgagtc gcttcgaaag tgagcaggtg aatctcgccg tgggcacggg
     1861 cacgggcacc ggcggcctgt cgacctcgct gccgaatgcg ttcaacgcga tcgcgagcgc
     1921 caacgtgttg ggcgacacga cgcccgatgg catgaacaac cgcggcatga gcctggtgcc
     1981 gagcgtctac tccgacacgg cggcgcggcg aagcgaaggc tacgaatt
//
LOCUS       DASPLT010000395         2852 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_86944, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000395 DASPLT010000000
VERSION     DASPLT010000395.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 2852)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2852)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2852
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_86944"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1466
                     /locus_tag="VEA63_04085"
     CDS             <1..1466
                     /locus_tag="VEA63_04085"
                     /inference="COORDINATES: protein motif:HMM:NF026235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="putative glycoside hydrolase"
                     /protein_id="HYD83199.1"
                     /translation="GASVYAGLAVCSVSAGNLCTATFESVSITTPDTFAPAFPRLGIY
                     AIGGDQSYPAAVRPVLAKFHVVIMSGAWETWDNGRAWTREDVVAGIKSASTIGTKVFQ
                     YVNFNESRPSGDPQIWFPSFFSLTNTNNWWLYNNGTSGTKTGSPYDASWWLLNLTHNA
                     PVDPVTGLRPYQAAAQFAYDEFVDAASPNAAPSLDGFFLDNVFWEPRTSGGNIGDWNR
                     DGTSDTNNAALQLAVRTGQKDFFDQLAMLMPGKLQLGNTADWGNASTTNVNAVAPLNG
                     ALHGGILEWATGRSFSTETWGGTETLIAWYKYLMDAAGDPKIVLFNQARLGPFTASDV
                     SVANPGADPYDSTSYQAMRYGMACALMNDGYYNACGYTHYATQSLIQWYDEFDAAGVG
                     TGYLGYPVAGANGDVQTGPRWPTKGTHGLWAREFSGGLVLMNPKGNGSQTVTISELGG
                     NTVWKHIDGTQAAVNDGSNVTGNITLAARDGLILLRR"
     gene            complement(1563..2384)
                     /locus_tag="VEA63_04090"
     CDS             complement(1563..2384)
                     /locus_tag="VEA63_04090"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HYD83200.1"
                     /translation="MPRNRPTQHRNTFGFCVWPVSPHGAMPAAHTHPDVEFNFLRGGQ
                     IAYLHGGKQHVVKAGQMGVFWGGVPHQTLGRTGNGIWVTLPLAWLLQWRLPGDLTHRL
                     LAGDLIQADADLSQVERWLGDYTAGGDRHRALQLELEAWLLRVALSIPARVRRQAATL
                     RGSTQIERIIGHIAAHYREAITVSDIAAAVKLHPKYLMRVFKQQCRIGVWEYLTRLRI
                     SHAQRLLVTTDARVSEIALDSGFGSSAAFYQAFTKHAGGEKPADYRTRHRIAGDT"
     gene            2479..>2852
                     /locus_tag="VEA63_04095"
     CDS             2479..>2852
                     /locus_tag="VEA63_04095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD83201.1"
                     /translation="MNTAPSVPQHSNLCATAPAVLVPEARVPTKMEEYLFDLQGFVVL
                     RGALSKDEVAACNATIDRIPRATPRNGWWGHVHREDHPEHRGISYQQIYESGPAFERL
                     IDHPSWINYVSLFVGGHDTFDW"
BASE COUNT          541 a          886 c          916 g          509 t
ORIGIN      
        1 tgggcgccag cgtgtatgcc gggctcgccg tttgcagcgt gtcggcgggc aatctctgca
       61 ccgcgacgtt cgagagcgtc tccatcacga caccggacac gttcgctccg gcattcccgc
      121 gacttggcat ttacgcgatc ggcggcgacc agtcgtatcc ggcggcggtg aggcccgtgc
      181 tggcgaagtt ccacgtcgtc atcatgagcg gcgcctggga aacctgggat aacggccgcg
      241 cctggacccg tgaggacgtg gtcgcgggaa tcaagtcggc gtcgacgatc ggcacgaagg
      301 ttttccaata cgtgaacttc aacgagagcc ggccctccgg cgatccgcag atctggttcc
      361 cgagcttctt cagtctcacg aacacgaaca actggtggct ctacaacaac ggcacgagcg
      421 gcacaaagac cggcagtccc tacgatgcga gctggtggct gctcaacttg acgcacaacg
      481 cgccggtcga tccggtgact ggccttcgcc cgtatcaggc cgcggcgcag ttcgcctatg
      541 acgaattcgt cgacgctgca tcgcctaatg ccgcgccgtc gctcgatgga ttctttctcg
      601 acaatgtgtt ctgggagccg cggacgagcg gcggtaacat cggcgactgg aaccgcgacg
      661 gcacatccga caccaacaac gcggcattgc agctcgccgt gcgcacgggg cagaaggatt
      721 tcttcgatca actcgcgatg ctgatgccgg gcaagcttca actcgggaac accgcggatt
      781 ggggcaacgc ctcgaccacg aacgtaaacg ctgtggcgcc gttgaacggc gcgctgcacg
      841 gcggcattct cgaatgggcg accggacgca gtttctcgac cgagacctgg ggcggcaccg
      901 agacgctcat cgcatggtat aaatatctga tggatgcggc cggtgacccg aagatcgtgc
      961 tgttcaacca agcccggctc ggaccattca ccgccagcga cgtgagcgtc gcgaatcccg
     1021 gcgccgaccc ttacgattcc acctcgtatc aggcgatgcg atacggcatg gcctgcgcgc
     1081 tgatgaacga cggctattac aacgcctgcg gctacacgca ctacgccaca caaagcctca
     1141 tccagtggta tgacgagttc gacgccgctg gcgttggcac aggctacctt gggtatccgg
     1201 tcgcgggcgc gaatggcgac gtgcagaccg gaccgcgctg gccgaccaag ggcacgcacg
     1261 gtctctgggc gcgcgagttc agtggcgggc tcgtgctcat gaatccgaag gggaacggtt
     1321 cgcagacggt gacgatctcc gaactcggcg ggaacaccgt ttggaagcac atcgatggca
     1381 cgcaagcggc ggtcaacgac ggttccaacg tgacgggcaa catcacgctc gccgcgcgcg
     1441 acggcctgat cttgctgaga cgttgacgag caccgcgcat cacgcggcga ctcgcctgca
     1501 atggccggct cggaaacgag ccggcttcat ttgcgcacgc tgcacgcgag cgcggcgcgc
     1561 gctcacgtgt cgcctgcgat ccgatggcgg gtgcggtaat cggcgggttt ttcgccgccg
     1621 gcgtgcttgg tgaaggcctg ataaaacgcg gccgacgaac cgaagccgct gtcgagcgcg
     1681 atttcgctta cgcgcgcgtc ggtcgtcacg agcaggcgtt gtgcgtgaga gatgcgcagg
     1741 cgcgtgagat actcccacac accgatgcgg cattgctgct tgaagactcg catcaggtat
     1801 ttgggatgaa gcttcaccgc ggcggcgata tcggagacgg tgatcgcttc gcgatagtgc
     1861 gcggcgatgt gaccgatgat gcgctcgatc tgggtcgagc cgcgaagggt ggcggcttgc
     1921 ctgcggactc tggccggaat cgaaagcgcc acgcgcagga gccaggcttc cagctccagt
     1981 tgaagcgcac ggtgccggtc gccaccggcg gtgtaatctc cgagccagcg ttcgacctgc
     2041 gagagatcgg catcggcttg gatcaaatcg ccggcgagca gccgatgcgt gagatcgccg
     2101 ggcaaccgcc actgcagcag ccaggcgagt ggcagcgtga cccagatgcc gttacccgtg
     2161 cggccgagcg tttgatgcgg cacgccgccc cagaagacgc ccatctgtcc ggctttgacg
     2221 acgtgttgct ttccaccgtg gagatacgcg atttgaccgc cgcgaagaaa attaaattca
     2281 acatccgggt gcgtatgcgc ggccggcatt gcgccgtgcg gcgacaccgg ccacacgcaa
     2341 aagccgaagg tattgcgatg ctgagtcggc cggttgcggg gcatgtggat gtcgtcaacg
     2401 ccggcaggtt accattcaag acaaaatgct gacgttgccc agaggacggt ggtgacagat
     2461 tccggtagag ttgcctcgat gaacaccgcc ccttccgttc cccagcattc aaatctctgc
     2521 gcgaccgctc cggcggtcct tgtccctgaa gcacgtgtgc cgacgaagat ggaggaatac
     2581 ctcttcgatc tgcagggctt tgtcgttctg cgcggcgcgc tgtcgaaaga cgaggttgca
     2641 gcctgcaacg caacgatcga tcgcattccc cgcgcgacgc cgcgcaatgg ttggtggggg
     2701 catgtgcatc gcgaggatca tcccgagcat cgcggcatca gctaccagca gatttacgag
     2761 tccggcccgg ccttcgagcg gctcatcgat catccgagct ggatcaacta tgtttctctt
     2821 ttcgtcggcg gccacgacac cttcgactgg ca
//
LOCUS       DASPLT010000396         1278 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_148191, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000396 DASPLT010000000
VERSION     DASPLT010000396.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1278)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1278)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1278
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_148191"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..330
                     /locus_tag="VEA63_04100"
     CDS             <1..330
                     /locus_tag="VEA63_04100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HYD83202.1"
                     /translation="RLPDVWSAADLPLGKDGDRVKIAGSVICRQRPGTAKGFVFISLE
                     DETGIANAVVVPDLFEARRMTINEEPTLCIVGRLQIQAGVIHVRAETIEALRLAELPA
                     QASHDFR"
     gene            610..1215
                     /locus_tag="VEA63_04105"
     CDS             610..1215
                     /locus_tag="VEA63_04105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376854.1"
                     /note="This protein belongs to the uracil DNA glycosylase
                     superfamily, members of which act in excision repair of
                     DNA. However, it belongs more specifically to UdgX branch,
                     whose founding member was found to bind uracil in DNA
                     (where it does not belong), without cleaving it, appears
                     to promote DNA repair by a pathway involving RecA, rather
                     than base excision; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UdgX family uracil-DNA binding protein"
                     /protein_id="HYD83203.1"
                     /translation="MANAWEKVRSDAKRCTRCPLYKIGTQTVFGEGPVKARVMMIGEQ
                     PGDQEDLAGRPFVGPAGKLLDRALVDAGIERDDVYVTNAVKHFKWERAGKRRLHKKPN
                     AREVAACRVWLDLELELIAPELVVALGATAAQALMGRTFRVTQQRGEVLTVPWAKAFL
                     ATVHPSSILRGPPEDREVNYAAFVKDLKMVAKALGKAGRGK"
BASE COUNT          235 a          274 c          551 g          218 t
ORIGIN      
        1 cggctgccgg atgtctggag cgcggcggat ttgccgttgg ggaaggacgg cgatcgcgtg
       61 aagatcgcgg gcagcgtgat ttgtcggcag cggccgggga cggcgaaggg ctttgtgttt
      121 atttcgctgg aagacgagac aggaatcgcg aatgcggtgg tggtgccgga tttgttcgag
      181 gcgcggcgga tgacgatcaa cgaggagccg acgttgtgca tcgtggggcg gctgcagatt
      241 caggcgggcg tgattcatgt gcgggcggag acgatcgagg cgttgcgact cgcggaattg
      301 ccggcgcagg cgtcgcatga tttccggtga agcgcctgcg aggggctcgc tggcggagat
      361 aatactttga accacgaagg gacacggatg aacgagccgc ggagttttga accacgaatg
      421 gacacgaagg gacacgaatg gaatcgacgg gaacggcttc gacactgccg cgggagagcg
      481 aacggcggtt ctattctaac cacgatggac gcgaatcgag gcggattgga atgagagggg
      541 gcagtgggat cgcgtgggat tcgtggcggg gcggcgtaac ttttcgttgg gggagcggtc
      601 tcggcggggg tggcgaatgc gtgggagaaa gtgcggagcg atgcgaagcg gtgcacgcgg
      661 tgtccgctct acaagatcgg cacgcagaca gtattcggcg aagggccggt caaagcgcgc
      721 gtgatgatga tcggcgagca gccgggcgat caggaggatt tggcggggcg gccatttgtc
      781 gggccggcgg ggaagttgct ggatcgtgcg ctcgtggacg cggggatcga gcgtgacgac
      841 gtttatgtga cgaatgcggt gaagcatttt aagtgggagc gcgcggggaa gcgccggctg
      901 cacaagaagc cgaatgcgcg cgaggtggcg gcgtgtcgcg tgtggctgga tctggagctg
      961 gagttgatcg cgccggagtt ggtggtggcg ctcggagcga cggcggcgca ggcgttgatg
     1021 gggcggacgt ttcgcgtgac gcagcagcgc ggcgaggtgc tgacggtgcc gtgggcgaaa
     1081 gcgtttttgg cgacggtgca tccgtcgtcg attttgcgcg ggccgccgga ggatcgggag
     1141 gtgaactatg cggcgttcgt gaaggatctg aagatggtgg cgaaagcgtt ggggaaagcg
     1201 ggccggggaa agtgaggcga gggcggggag gtatgggacg gaggaagggc gagagcaggc
     1261 aggcgcggag gcctgccg
//
LOCUS       DASPLT010000397         1955 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_148221, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000397 DASPLT010000000
VERSION     DASPLT010000397.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1955)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1955)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1955
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_148221"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            9..554
                     /locus_tag="VEA63_04110"
     CDS             9..554
                     /locus_tag="VEA63_04110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HYD83204.1"
                     /translation="MRAGDQIAARELVEHLYPQVIRIVRARLPRRVPEEDLAQEIFLK
                     MFSRIDQYQGAVPFAHWVSRIAVTTCIDHLRAQQRRPEFRMADLSENEANLIDAVLTT
                     EKAPTQDDALEAHELVHKLLGQLRPDDRLVLQMLDLERRTIAEISEQTGWNTSLVKVR
                     AFRARRKLQKLFKELQRKERS"
     gene            551..850
                     /locus_tag="VEA63_04115"
     CDS             551..850
                     /locus_tag="VEA63_04115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD83205.1"
                     /translation="MKPEDQAWQKLVAAARFARDDRDTAAPYGFATRVAALAMAEQRQ
                     APAGAWVERFSWRALMVAGLLAVGGVATNYASPMPANGDDEVLDETTVTAVFDLS"
     gene            853..1233
                     /locus_tag="VEA63_04120"
     CDS             853..1233
                     /locus_tag="VEA63_04120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD83206.1"
                     /translation="MNKSWKVVLAFAGVFLAGAIFGGALAPHLKRFERPQPRPPLAER
                     MMQRFDEQLQLTAEQKPKVRPIVDRMSEETLRMRRESSVAFRAMMDRMTAELARELTP
                     EQRVKLDEMRAYYRERAEQRNRGR"
     gene            1361..1459
                     /gene="ffs"
                     /locus_tag="VEA63_04125"
     ncRNA           1361..1459
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="VEA63_04125"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            1584..>1955
                     /locus_tag="VEA63_04130"
     CDS             1584..>1955
                     /locus_tag="VEA63_04130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012377358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HYD83207.1"
                     /translation="MSSTGYQVIARKWRPQTFDDVVGQDHVVRTLKNAIARNRIAHAY
                     LFVGPRGTGKTSTARIFAKALNCTGGPKADFDPADPACVAIAEGSHLDVIEIDGASNN
                     GVDQVRDLRETVRYAPAQGKFK"
BASE COUNT          356 a          523 c          735 g          341 t
ORIGIN      
        1 tggcgcgggt gcgcgcgggc gaccagatcg cggcgcgcga gctggtggag cacctttatc
       61 cgcaggtcat tcgaatcgtg cgcgcgcggc ttccgcggcg ggtgcccgaa gaagacctcg
      121 cgcaggagat tttcctaaaa atgttttcgc ggatcgatca gtatcaggga gcggtgccgt
      181 tcgcgcattg ggtgtcgcga atcgcggtga cgacgtgcat cgatcatctg cgcgcgcagc
      241 agcggcggcc ggagtttcgg atggcggatt tgtcggagaa cgaggcaaac ctgatcgacg
      301 cggtgctgac gacggagaag gcgccaacgc aggacgacgc gctcgaggcg cacgagctcg
      361 ttcacaagct gctcgggcaa cttcgcccgg acgatcggct ggtgttgcag atgctcgatc
      421 tggagcggcg gacgattgcc gagatcagcg agcagacagg atggaacacg tcgctggtga
      481 aagtgcgggc gtttcgcgca cgacggaagt tgcagaagct ttttaaggag ttacaacgaa
      541 aggagcgatc atgaaaccgg aagaccaagc gtggcagaaa ctcgtggcgg cggcgcggtt
      601 cgcgcgcgac gaccgcgaca cggcggcgcc gtatggtttc gcgacgcggg tggcggcgct
      661 ggcgatggcg gagcagcggc aagcgcccgc gggtgcgtgg gtggagcgat tctcctggcg
      721 ggcgttgatg gtagccgggc tgctggcggt cggcggcgtc gcgacgaatt acgcatcacc
      781 gatgccggcg aatggcgatg atgaggtgtt ggatgaaacg accgttacgg cggtgttcga
      841 tctttcgtga cgatgaacaa gtcgtggaaa gtcgtgctcg cgtttgccgg agtgtttctg
      901 gccggggcga ttttcggcgg agcgctggcg ccgcacttga agcgtttcga gcggccgcag
      961 ccgcgtccgc cgctggcgga gcggatgatg cagcggtttg acgagcagtt gcaattgacg
     1021 gcggagcaga agccgaaggt gcgtccgatc gtggaccgga tgagcgagga aacgctgcgg
     1081 atgcgccgcg agagctcggt ggcgtttcgc gcgatgatgg atcggatgac ggcggaactg
     1141 gcgcgcgagc tgacgccgga acagcgcgtg aagctggacg agatgcgcgc gtattatcgg
     1201 gagcgcgcgg agcagcggaa tcgtgggcgg tgaacgccgg cgtggcgacg ggttgcacgc
     1261 gtgcgcgcaa cttacgattg ggcaggcagg cgcggaggcc ggccgctacg gttttcaatt
     1321 agcccttgcg ccgcggaggc gacgcagcat ggtgcgcgtc ggccaggtgc catgcatggg
     1381 caggcgcgtg aactccgcca ggaccggaag gtagcaacgg caacgacgtt ctcctagtgc
     1441 cgtggagtgc ctggccacta ttttttcttc tccgctgtcg cggggaaaag ttgagagctg
     1501 agggttgaga ggtgagagac cggtctgcgg acgcgttttc ggctttcaac tcaccgccct
     1561 caactctcaa ctctcaactt cacgtgagct ccaccggtta ccaagtcatc gcgcgcaagt
     1621 ggcggccgca gacgtttgac gacgtcgtgg ggcaggatca cgtcgtgcgg acgttgaaga
     1681 acgcgatcgc gcgcaaccgg atcgcgcacg cgtatctgtt cgtggggccg cgcgggacgg
     1741 gcaagacgtc gacggcgcgg atttttgcga aggcgttgaa ttgcacgggc gggccgaagg
     1801 cggatttcga tccggcggat ccggcgtgtg tggcgattgc ggaaggttcg catctcgatg
     1861 tgatcgagat cgacggcgcg tcgaacaacg gcgtcgatca agtgcgcgat ctccgggaaa
     1921 ccgtccgcta cgcgcccgcg cagggcaaat tcaag
//
LOCUS       DASPLT010000398         1025 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_189564, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000398 DASPLT010000000
VERSION     DASPLT010000398.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1025)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1025)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1025
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_189564"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..684)
                     /locus_tag="VEA63_04135"
     CDS             complement(<1..684)
                     /locus_tag="VEA63_04135"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HYD83208.1"
                     /translation="MKTLRLGIIGGGLMGKETASAVARWCNLDDVGVHAELTAVCDLQ
                     PAVLDWFRRIPSVKLFTSDAAQLLASKEVDAVYVAVPHHLHESLYVQVLRAGKDLLAE
                     KPFGVDLAAAERVRTEAEKLGRFVRCSSEFPFWPGPQRIMSEVRANKCGRLLEVRSGF
                     LHASDLDPTKPINWKRQAKFCGAAGVMNDLGMHAVHVPLRMGWLPSRIYARLQKIVAT
                     RPDGSGGTAP"
     gene            complement(693..>1025)
                     /locus_tag="VEA63_04140"
     CDS             complement(693..>1025)
                     /locus_tag="VEA63_04140"
                     /inference="COORDINATES: protein motif:HMM:NF013916.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD83209.1"
                     /translation="GDLKKITGLVRQAVELGADVIKADPTDNVADYHKVIETAAGVPV
                     LVRGGGKVTDREILVRTEELLKQGAAGIVYGRNVIQHAKPAAITKALMALLHDGLSAD
                     EAFAKHLA"
BASE COUNT          186 a          325 c          318 g          196 t
ORIGIN      
        1 cggcgctgtg ccgccgctcc cgtccggtcg cgtcgcgacg atcttctgca gccgcgcgta
       61 gatgcgcgac gggagccagc ccatccgcag cggcacgtgc acggcatgca tgccaaggtc
      121 gttcatcacg ccggctgccc cgcagaattt tgcctgacgc ttccaattga tgggcttggt
      181 gggatcgaga tcgctggcgt gaaggaagcc gctccgcacc tcgaggaggc gaccgcattt
      241 gttcgcacgc acttcgctca tgattcgttg agggccgggc cagaacggaa actccgagga
      301 gcagcgaacg aatcgcccga gcttctccgc ttcggttcgc actcgctctg ccgcggctaa
      361 atcgaccccg aaaggttttt ccgcgagcaa atctttgccg gcgcgaagca cctgaacata
      421 aagtgactcg tgcaagtgat gcggcaccgc gacgtagacg gcgtcgacct ccttcgaggc
      481 gagtagctgc gctgcatccg aggtgaagag cttcacggag ggaatgcgac gaaaccagtc
      541 gagcaccgcg ggctgcaaat cacagacggc agtgagctcc gcatgcacgc cgacatcgtc
      601 gagattgcac cagcgggcga cggcggaggc ggtttctttg cccatcaaac cgccgccgat
      661 gatgccgaga cgtagagttt tcatgaccgt gttcaggcga gatgtttcgc gaatgcctcg
      721 tcggccgaga gaccgtcgtg caagagtgcc ataagcgcct tcgtgatcgc ggccggcttc
      781 gcgtgctgga tcacgttgcg gccatacacg atgccggcgg cgccctgctt cagcagctct
      841 tcggtgcgaa caagtatttc gcgatcggtc acttttccgc caccgcgcac gagcaccgga
      901 acgccggcag cggtttctat gactttgtga tagtcggcta cgttgtccgt cgggtccgcc
      961 ttgatcacat cggcgcccag ctcgacggcc tgccgcacga gcccggtgat tttcttcagg
     1021 tcgcc
//
LOCUS       DASPLT010000399         1196 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_27565, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000399 DASPLT010000000
VERSION     DASPLT010000399.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1196)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1196)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1196
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_27565"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            171..>1196
                     /locus_tag="VEA63_04145"
     CDS             171..>1196
                     /locus_tag="VEA63_04145"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HYD83210.1"
                     /translation="MPASAPVIAYLFTTFPKSTETFLQREIVAMRAHGVALRIYSLWG
                     GGGTFRGLEVRRFNKWRLFTLLWLIPYESWRRPEVLRQLLRGLVTRRAPSWLNFWENM
                     LGAGFACVFAREFRRDPPALVHAAWAGGPATAAWLLWRMDGNRFSAAAHAYDIFEYGG
                     DWWLREKLEAAAFVHTSTEMGREALKAGGVAAEKIVCIRRGLDRLPSLKRLRSHRSPL
                     LLVCIARLVEKKGLRHQLRVYAALQAAGIDFEARIVGDGPLRAELEQLAGHLGIASKV
                     TFTGHLAQHDVWAQLAWADAMLHTGVIALSGDRDGLPNVIPEAMSIGVLVVTSPVAAT
                     TEAIIDGE"
BASE COUNT          179 a          316 c          466 g          235 t
ORIGIN      
        1 ttgggaacga agtggcgaag gcgaatttgc ggcggctgcc gtttccaccg ccgaacggca
       61 accgccacgg ggcgaacggc ggcggcggac ggtagcgcgc gaagcgggcg gcgaaacgga
      121 atgtttaccg cttggccgcg cgagtgcggg cgcgctagcg tggcgtcatc ttgcccgctt
      181 ccgcgcctgt catcgcttat ctgttcacga ccttcccgaa atcgacggag acttttctcc
      241 agcgcgaaat cgtggcgatg cgggcgcatg gcgtcgcgct gcggatctat tcgctgtggg
      301 gcggcggcgg aacgtttcgc gggctcgagg tgcggcggtt caacaagtgg cggctgttca
      361 cgctgctttg gctgattcct tacgaatcgt ggcggcggcc ggaggtgttg cggcaacttt
      421 tacgcggact cgtgacgcgt cgtgcgccct cgtggctgaa cttttgggag aacatgctgg
      481 gcgcgggttt cgcgtgtgtg ttcgcccggg agtttcgtcg cgatccgccg gcgctcgttc
      541 acgcggcgtg ggcgggcggg ccagcgacgg cggcgtggtt gttgtggcgc atggatggga
      601 atcgcttcag cgcggcggcg catgcgtatg atatcttcga atacggcggc gactggtggc
      661 tgcgcgagaa gctcgaggcg gcagcgttcg tgcacacgtc gacggagatg ggccgcgagg
      721 cgttgaaggc gggcggggtg gcggcggaga aaatcgtctg catccggcgc ggactggatc
      781 ggctgccgtc gttgaaacga ctgcggtcgc atcgttcgcc gctgttgctg gtgtgtattg
      841 cgagactggt ggagaagaag gggctacgtc atcaactgcg ggtttacgcg gcgttgcagg
      901 cggcggggat cgatttcgaa gcccggatcg tgggcgatgg accgctgcga gcggagttgg
      961 aacaactcgc cgggcatctc gggatcgcgt cgaaggtgac gttcacgggg catctcgcgc
     1021 agcacgatgt atgggcgcaa ctcgcgtggg cggatgcgat gttgcacacg ggcgtgatcg
     1081 cgttgagcgg cgatcgcgat gggttgccga atgtgattcc cgaagcgatg tcgatcggtg
     1141 tgctcgtggt gacgtcgccg gtcgcggcga cgaccgaggc gattatcgat ggcgaa
//
LOCUS       DASPLT010000400         1082 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_189612, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000400 DASPLT010000000
VERSION     DASPLT010000400.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1082)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1082)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1082
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_189612"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1082
                     /locus_tag="VEA63_04150"
     CDS             <1..>1082
                     /locus_tag="VEA63_04150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD83211.1"
                     /translation="TDPSIFDFYNKLLDGDNKREWADWDAYQANVSQTFLGNSVGYEL
                     AYFKQTMDRGQWGILGWDNFIAMDINATTSENTPNPDVGRAYIQAENRDIGNYSLHSE
                     REAKRATLFGEYDFQKRHSDNLWTRILGTHRINGVASEEGVKSDRRGFRLGDLDNASK
                     LALAPRGGIEGDQGITSAFRYYLSDDLRGQPSAANSNISNISQPAFVREGGLINVRYW
                     DNTWIAGPEVDPGAEWTNPFGDVTFQSENPANYRGWAQRDARFITIMSKDRQLDMSAK
                     DYLTSSGSLSDFKVKSLVGVWQAYLWDKSIIGTYGYREDRFRRFSYTSSDRQGTGRAD
                     TGGADLRPTSYNYANPDGTIENMRTIT"
BASE COUNT          246 a          347 c          298 g          191 t
ORIGIN      
        1 accgatcctt cgatcttcga tttctacaac aagcttctcg acggcgacaa caaacgcgaa
       61 tgggccgact gggacgccta tcaagccaac gttagccaga cgttcctggg caactcggtt
      121 ggctacgagc tcgcctactt caagcagaca atggaccgcg gccagtgggg tattctcgga
      181 tgggacaact tcatcgcgat ggatatcaac gcgaccacgt ccgagaacac gccgaacccc
      241 gatgtcggtc gcgcctatat tcaggccgaa aaccgcgaca tcggcaacta ctcgctccac
      301 tccgagcgcg aagcgaaacg cgccacgctg tttggcgaat acgatttcca aaagcggcat
      361 agcgacaacc tctggacgcg cattctcggc acgcatcgca tcaacggcgt cgccagcgag
      421 gaaggcgtca aatcggatcg tcgcggcttt cgcctcggcg atctggacaa cgcctcgaag
      481 ctcgccctcg cgccgcgcgg cggcatcgaa ggcgaccaag gtatcaccag cgcttttcgc
      541 tactacttga gcgacgatct ccgcggacag cccagcgctg ccaacagcaa catctcgaac
      601 atctcgcagc cggcgttcgt gcgcgaaggc ggtctgatca acgttcgcta ttgggacaac
      661 acgtggatcg ccggacccga ggtcgatccc ggcgccgagt ggacgaatcc ttttggtgac
      721 gtgacgttcc agtccgaaaa cccggcgaac tatcgcggtt gggcgcaacg cgacgcgcgg
      781 tttatcacca ttatgagcaa ggatcgccag ctcgacatgt ccgcaaaaga ttacctcacg
      841 agcagcggct cgctcagcga tttcaaagtc aagtcgctgg tcggagtctg gcaggcttac
      901 ctgtgggaca aatcgattat cggcacctat ggttatcgcg aagaccgatt ccgccgcttc
      961 tcctacacaa gctcggatcg ccaaggcacc ggacgcgcgg acacgggcgg cgccgatctt
     1021 cggccgacca gctacaatta cgcgaatccc gatggcacga tcgagaacat gcgcaccatc
     1081 ac
//
LOCUS       DASPLT010000401         1182 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_105982, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000401 DASPLT010000000
VERSION     DASPLT010000401.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1182)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1182)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1182
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_105982"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..612
                     /locus_tag="VEA63_04155"
     CDS             <1..612
                     /locus_tag="VEA63_04155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HYD83212.1"
                     /translation="ILLLDEPLSNLDAKLRLEMRTEIRRVCKEFKLTTVYVTHDQKEA
                     LSISDRMAILESGRILQVGTPREIYRRPARKTVAHFIGETDFIAGTVTSVSDGRVKVH
                     TAIGDFEGVAGDAKAELKANDVVTLSIRPECWALSREAPARNAVKGRIGEAVYLGEVA
                     QYDFVAGGATLKIFELNPRFVGSASRGELYASVEPEDVVVLAE"
     gene            821..>1182
                     /locus_tag="VEA63_04160"
     CDS             821..>1182
                     /locus_tag="VEA63_04160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter substrate-binding protein"
                     /protein_id="HYD83213.1"
                     /translation="MLKRVIIILALVATVALPFLLRPKQRAPERADDTITIITPHNEA
                     IRHEFAVGFKRWYREKTGRSVALDWRVLGGTADITRFLESEYVASFRNYWTNTLGRAW
                     SSDVQGGFANARLSADAPA"
BASE COUNT          226 a          277 c          452 g          227 t
ORIGIN      
        1 atcctgctgc tcgacgagcc gctgtcgaat ctcgacgcga agctgcgctt ggaaatgcgc
       61 acggaaatcc ggcgggtgtg caaggagttc aagctgacga cggtttacgt gacgcacgat
      121 cagaaggagg cgctgtcgat ctcggatcgg atggcgattt tggagagcgg gcggattttg
      181 caggtgggca cgccgcgtga gatttaccgg cggccggcgc gaaagacggt ggcgcatttc
      241 atcggcgaaa cggatttcat cgcgggcacg gtgacgtcgg tgagcgacgg tcgcgtgaag
      301 gtgcacacgg cgatcgggga tttcgaaggc gtcgccggcg atgcgaaggc ggagttgaag
      361 gcgaacgatg tcgtgacttt gtcgatacgc ccggagtgct gggcgttgag tcgcgaggcg
      421 ccggcgcgga atgcggtgaa ggggcggatc ggcgaagcgg tgtatctcgg cgaggtggcg
      481 caatacgatt tcgtggcggg aggcgcgacg ttgaagattt tcgagctgaa tccgcggttt
      541 gtcgggtcgg cgtcgcgggg cgagttgtat gcgagtgtcg agccggagga cgtggtggtg
      601 ctcgccgagt aaccatgcgc ggcggggcaa cgtggccgaa tgcaggcagg caaccccgaa
      661 tcgagctttt gaacgccaga gccgagccag gttttaaccg cggatgaaca cggatatgag
      721 gagaagaatt tttttaacca cgaatggaca cgaatggaca cgaagcagaa cgtggaggtg
      781 gtgtgatgcg gctctgtttg ttcgtgattg ctgctgagtc atgttgaagc gggttattat
      841 catcttggcg ttggtggcga cggtggcgct gccgtttttg ctgcggccga agcagcgggc
      901 gccagagcgg gccgacgaca cgatcacgat catcacaccg cacaacgagg cgatccggca
      961 cgagttcgcg gtcggtttca aacggtggta tcgggagaaa acggggcgca gcgtggcgct
     1021 cgactggcgg gtgctcggcg gcacggcgga catcacgcgt tttttggagt cggagtatgt
     1081 ggcgtcgttt cgtaattact ggacgaatac gttgggcagg gcgtggagca gcgacgtgca
     1141 aggcgggttc gcgaatgcgc gactgagcgc cgatgcgccg gc
//
LOCUS       DASPLT010000402         1508 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_292131, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000402 DASPLT010000000
VERSION     DASPLT010000402.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1508)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1508)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1508
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_292131"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..217)
                     /locus_tag="VEA63_04165"
     CDS             complement(<1..217)
                     /locus_tag="VEA63_04165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD83214.1"
                     /translation="MNPAPGPLNGLFGRRPELRSCETSIVEAFETLRRCFEREGRVYL
                     CGNGGSGADCEHWAGELLKGFRAKRSPR"
     gene            complement(488..1045)
                     /locus_tag="VEA63_04170"
     CDS             complement(488..1045)
                     /locus_tag="VEA63_04170"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025004.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 2-dehydrogenase subunit 3 family
                     protein"
                     /protein_id="HYD83215.1"
                     /translation="MEMTRRQAVIKMAFAMGASVIGPRLLAAQFGGEPGLPASFSEEE
                     RALLDEIGETIIPATDVPGAKAVGIGAFIATMVQDCYPSEVQAAVRNGLSKIASLHEA
                     RFGVSFMSGAAKDRTELLNELDREQKAAHARGGPLHYFRVLKELTILGYFTSKIGCTQ
                     ALRFVEVPGSYDGDAPYRKGEKAWF"
     gene            complement(1059..>1508)
                     /locus_tag="VEA63_04175"
     CDS             complement(1059..>1508)
                     /locus_tag="VEA63_04175"
                     /inference="COORDINATES: protein motif:HMM:NF017050.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC oxidoreductase"
                     /protein_id="HYD83216.1"
                     /translation="WTIGLSAFGETLPYHENRVTLERSVKDKWGLPVLAMDCAIKENE
                     MKMREEMKSDAAEMLEAAGVKEVAIYDKGYNFGRGIHEMGSARMGRDPKTSVLNAHNQ
                     VWDARNVFVTDGACMTSASCVNPSLTYMALTARAADFAVAELKRGNL"
BASE COUNT          286 a          506 c          408 g          308 t
ORIGIN      
        1 gtcgcggcga tcgcttcgca cgaaacccct ttagcaactc gccggcccaa tgctcgcaat
       61 ccgccccgct cccgccattg ccgcagagat acacgcgacc ttctcgctcg aaacaacgtc
      121 gcagcgtttc gaacgcttcc acaatcgacg tttcgcagct ccgcaattct ggccgccggc
      181 cgaacaatcc gtttagcggc cctggggctg gattcatgag tttcggggtg cttgttggcg
      241 gtgcatacac gcgaggatga cagagagccg gggggacgtc tttgccgaaa tcggcacttt
      301 tattgtcgtt tcgcctgaag agggaaagac actgccgctt cgacatggcc gttccagcgc
      361 acgtcttcgc gtcgaaattg tcctggcgtc acgcctgtca cttggcggaa cggcttctcc
      421 gctcacaaac cggcacgccc ggcggcgggc cgctacgaat agtcggctac ggcttatttg
      481 aaacgctcta aaaccaagcc ttctcgcctt tgcgatacgg cgcgtcgcca tcgtagctgc
      541 ccggcacttc cacgaaacgc agcgcctgcg tgcagccgat cttggaagtg aaataaccga
      601 ggatcgtaag ctctttcagg acgcgaaaat agtgcagcgg ccctccgcgg gcatgcgccg
      661 ccttctgctc gcgatccagc tcgttgagca actccgtgcg gtccttcgcg gcgccgctca
      721 tgaacgacac gccgaaacgc gcttcgtgca gcgaagcgat tttcgacaac ccattccgca
      781 ccgccgcctg cacctcactc ggataacaat cctgcaccat ggtcgcgatg aacgcgccga
      841 tgccgaccgc tttcgcgccc ggcacgtccg tagcgggaat gatcgtctca ccgatttcgt
      901 ccagcagcgc gcgctcttcc tcggagaacg atgccggcaa accgggctct ccaccgaatt
      961 gtgcagcgag caaacgcggg ccgatcacag atgctcccat cgcgaaggcc attttgatca
     1021 ccgcctgccg acgtgtcatt tccatgatcg aaaacggctc aaagattgcc ccgcttcagt
     1081 tcggccaccg cgaagtctgc cgcccgcgcg gtcagcgcca tataagtgag cgatgggttc
     1141 acgcaggacg ccgaggtcat gcaggcgccg tccgtgacga aaacatttct cgcatcccac
     1201 acctggttgt gcgcgttgag caccgaggtt ttcggatcgc ggcccatgcg ggcggaaccc
     1261 atttcatgaa tcccgcggcc gaaattgtaa cctttgtcgt agatcgcgac ctccttcacg
     1321 ccggccgcct cgagcatttc ggcagcatcg ctcttcatct cttcgcgcat cttcatttcg
     1381 ttctccttga ttgcgcagtc catcgcgagc acggggagac cccacttgtc cttcacgctt
     1441 cgctcaagcg tgacgcggtt ctcgtgatac ggcagcgttt caccgaatgc cgaaaggccg
     1501 atcgtcca
//
LOCUS       DASPLT010000403         1023 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_86989, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000403 DASPLT010000000
VERSION     DASPLT010000403.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1023)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1023)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1023
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_86989"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1023
                     /gene="ptsP"
                     /locus_tag="VEA63_04180"
     CDS             <1..>1023
                     /gene="ptsP"
                     /locus_tag="VEA63_04180"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009511361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HYD83217.1"
                     /translation="RFEKALLVTRQQIAKIQDEVEKNLGKDESMIFDAHLLVLEDQAL
                     IGETIREFEATGRNIETCFNKVSSRYIQAFSEIDDEYLRERAGDLRDVAQRVLQNLLG
                     QAENSLSRLADKRIVIANDISPSDSASIDRSAALGIVTDSGSKTSHAVIVARSMKVPA
                     VVGVRDLSKRVQHGDWVILDGYDGVVIINPSESTLFRYGKIQEQKKTFESRLLKANQE
                     PAMTLDGVAVTLMANIEKSDEVRLVKDYNAAGVGLFRTEYIFLSSSRIPTEQEQFLAY
                     KAVAEGVAPNSVIIRTLDLGGDKPMAGNPDLFPKENNPFMGFRAIRFCLEHVDIFKDQ
                     LRAILMA"
BASE COUNT          205 a          249 c          371 g          198 t
ORIGIN      
        1 cggtttgaaa aggcgctgtt ggtgacgcgg cagcagatcg cgaaaattca ggacgaggtg
       61 gagaagaatt tggggaagga tgagtcgatg atcttcgacg cgcatctcct ggtcctggag
      121 gaccaggcgt tgatcggcga aacgatccgc gagttcgagg ccacgggccg gaacatcgag
      181 acgtgtttca acaaagtctc ctcgcgctac atccaggcct tttcggaaat cgacgacgaa
      241 tacctgcgcg agcgcgcggg ggacttgcgc gacgtggcgc agcgggtgtt gcagaatttg
      301 ctggggcagg cggagaactc gctgtcgcgg ctggcggaca agcggatcgt gatcgcgaac
      361 gacatctcgc cgtcggattc ggcgagcatc gatcggagcg cggcgctcgg gatcgtgacg
      421 gattccggga gcaagacgag tcatgcggtg attgttgcgc gttcgatgaa agtgccggcg
      481 gtggtcggcg tgcgggattt atcgaagcgc gtgcagcacg gcgactgggt gattctcgac
      541 ggctacgacg gcgtggtgat catcaatccg tcggagagca cgctgttccg ctacggcaag
      601 atccaggagc agaagaagac gttcgagtcg cgtttgctga aggcgaacca ggagccggcg
      661 atgacgctcg acggcgtggc ggtgacgctg atggcgaaca tcgagaagtc ggacgaggtg
      721 aggctggtga aggattacaa tgcggcgggc gtggggttgt ttcgcacgga gtatattttt
      781 ctcagttcgt cgcggatccc gacggagcag gagcagtttc tcgcctacaa ggcggtggcg
      841 gaaggcgtgg cgccgaactc ggtgatcatt cgcacgctgg acctcggcgg cgacaagccg
      901 atggcgggga atccggatct gtttccgaag gagaacaatc cgttcatggg atttcgtgcg
      961 atccggttct gtctcgagca cgtggatatt ttcaaggacc agttgcgcgc gatcctgatg
     1021 gcg
//
LOCUS       DASPLT010000404         1719 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_127510, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000404 DASPLT010000000
VERSION     DASPLT010000404.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1719)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1719)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1719
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_127510"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1699)
                     /locus_tag="VEA63_04185"
     CDS             complement(<1..1699)
                     /locus_tag="VEA63_04185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD83218.1"
                     /translation="MFVVFRKKATLTERTIAPPAEAKTLTALDQDWSVRFQENLGAPP
                     ELRLAKLAPWSEHPDDGVKYFSGAAVYRKNLEAPRDWFRADTGLFLDFGRVGDIADVT
                     INGRSFGLVWHPPYEVDVTGVLQPGDNLVEIKVTNQWTNRLIGDRAAPAEKRVFGEAA
                     ALPNRMRGPEPKLPESGLIGPVKLVVRAMNRVADNPDGKVAGIAANYTEALAGDPMLP
                     DPLTLANGQPVKDAKTWFDQRRPEILRVFEQNQFGRAPAQAKDISFEVIEKGAPAFDG
                     KALRKQVTIYFSKEKANGPYADVLLYLPANATAPVPVLLCANFSANNLAVDDLGVKVG
                     RIWNAEQKQRMPATAGRRFGRINVLDTIERGFGIATVNYADFDPDAPGAIGSGVRQLY
                     LEPGQNEPAADEWGAISAWSWGLSRVLDYFETDPQVNAKRVALFGVSRLGKTVLWTGA
                     RDSRFALVIASCSGEGGAALSRRNYGETVAHLTAPTRYPYQFAANYAKWAGKPAESPV
                     EANLLVSLIAPRPLLLQTGDTDIWSDPKGEFLAALAATPVYALLGESGLRTAQMPQPG
                     "
BASE COUNT          303 a          546 c          543 g          327 t
ORIGIN      
        1 gtccgggttg cggcatttgc gcggtgcgca atccggactc gccgagcaac gcgtaaaccg
       61 gcgtggccgc aagcgcggcg agaaattcgc ctttgggatc ggaccagatg tccgtgtcgc
      121 ccgtctgcag tagcagcggt cgcggtgcga tgagcgacac gagcaggttc gcttcgacgg
      181 gagactcggc gggtttgccc gcccatttcg catagttcgc ggcgaattga taaggatagc
      241 gcgtcggtgc ggtgaggtgc gcgacggtct cgccatagtt acgccgactg agcgccgcgc
      301 cgccttcgcc ggaacaactc gcgatcacga gcgcgaaacg cgaatcgcgt gcgccggtcc
      361 agagcacggt tttgccgagg cgcgatacgc cgaacagcgc gacgcgtttg gcgttcacct
      421 gtggatcggt ttcgaagtaa tcgagcacgc ggctcagccc ccaactccac gccgagatcg
      481 caccccactc gtccgccgcc ggctcgttct gaccaggctc gagataaagc tgccgcacgc
      541 cggacccgat cgcacccggc gcatcgggat cgaaatcggc gtagttcacg gtcgcgatgc
      601 cgaagccgcg ttcgatcgta tccagaacgt tgatacggcc aaaccgccgg ccagccgtgg
      661 ccggcatgcg ttgtttttgt tccgcattcc agatgcggcc gaccttcacg ccaagatcgt
      721 cgacggcgag attgttggcc gaaaagttcg cgcagagcag caccggcacc ggcgcggtcg
      781 cgtttgccgg aaggtagaga agaacatcgg catacggccc gttcgctttc tccttggaga
      841 agtaaatcgt cacctgcttc ctcaacgcct ttccgtcgaa cgcaggggcg cccttctcga
      901 taacttcgaa gctgatatcc ttcgcctgag ccggcgcgcg tccgaactgg ttctgttcga
      961 aaacgcgcag gatctcgggc cgtcgttggt cgaaccacgt tttcgcatcc ttcaccggct
     1021 ggccatttgc gagcgtcagc ggatcgggca gcatgggatc gcccgcgagc gcttcggtgt
     1081 agttcgccgc gatgccggcg accttgccgt cggggttgtc agccacgcgg ttcatcgcgc
     1141 ggacgacgag cttcacggga ccgatcaacc ctgactccgg cagtttgggc tccggaccgc
     1201 gcatgcgatt cggcagcgcg gcggcttcgc cgaacacgcg tttttcagca ggcgcggctc
     1261 ggtcgccgat cagccggttt gtccactggt tggtgacttt gatttcgacg aggttgtcgc
     1321 ccggctgcag cacgcccgtc acgtccacct cataaggcgg atgccagacc aggccaaacg
     1381 agcgaccgtt gatggtcacg tccgcgatgt cgccaacgcg gccgaaatcg agaaagagcc
     1441 ctgtgtcggc gcggaaccaa tcgcgcggtg cctcgaggtt cttccgataa accgccgcgc
     1501 cggaaaaata tttcacgccg tcgtcggggt gttccgacca tggtgcaagt ttcgccaaac
     1561 gcaattccgg cggcgcgccg agattctcct ggaagcgcac ggaccaatcc tgatcgagcg
     1621 cggtgagcgt tttcgcttcc gccggcggtg cgattgttct ttcggtgagc gtcgcctttt
     1681 tccgaaagac aacgaacacg gagtcgtgtc cgccgagac
//
LOCUS       DASPLT010000405         1252 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_169273, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000405 DASPLT010000000
VERSION     DASPLT010000405.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1252)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1252)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1252
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_169273"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..502
                     /locus_tag="VEA63_04190"
     CDS             <1..502
                     /locus_tag="VEA63_04190"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HYD83219.1"
                     /translation="RSFYVLFATAALALLLWQWRPMPAPVWTVEEPLYAGILWALCAA
                     GFGTVLLSTFLINHFELFGLRQVWANFTKHTPPEQAFTTPLFYKYIRHPLYLGFIIAF
                     WATPQMSVGHLLFAIATTGYIFVGIFFEERDLVAHFGERYRQYTRDVGMLVPKLRAGG
                     KRTKQA"
     gene            740..>1252
                     /locus_tag="VEA63_04195"
     CDS             740..>1252
                     /locus_tag="VEA63_04195"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HYD83220.1"
                     /translation="MKALEGKVAIITGASFGIGRAAAKLFAAEGARLVLVGRRPDPLN
                     AVAAEIASAGGECVVLAGDVAHEDTARDAVAAAERAFGGLDIAFNNAGSLGKMGPVTD
                     IAVSGWREAIDANLTGAFFAARHQIPAMLKRGGGSLIFTSTFVGHTAGFPGMSAYAAS
                     KAGVIGLMRNL"
BASE COUNT          210 a          425 c          378 g          239 t
ORIGIN      
        1 gcgcagcttc tatgtgctgt tcgcgaccgc cgcgctcgcg ctgctgcttt ggcagtggcg
       61 acccatgccg gcgccggtat ggaccgtcga agagccgctc tatgcgggca tactctgggc
      121 gctctgcgcc gccgggttcg gcaccgtgct gctctccacg ttcctgatca atcatttcga
      181 gctgttcggc ctgcgccaag tctgggcgaa cttcaccaaa cacacgccgc ccgagcaagc
      241 gttcacgacg ccgctcttct acaaatacat ccgccatccg ctctatctcg gcttcatcat
      301 cgccttctgg gcaacgccgc aaatgagcgt cggccacctt ctcttcgcca tcgccaccac
      361 cggctacatc ttcgtcggca ttttcttcga ggaacgcgac ctggtcgctc atttcggcga
      421 acgctatcgg caatacacgc gcgatgtcgg catgctggtg ccgaagctgc gcgcaggcgg
      481 caagcggacg aagcaggcgt gagctagaat acgactttga cgcgcgtcgc gtttgctcga
      541 cgcaaaaaac tcacccctcc ccggaggctc cggcgccgtg tccatcacgt cgcgcacgat
      601 tgtgcatagt gtttccgctt cgtgcttggg cgcgtgcacc tgcagcgagc cgcgccgctc
      661 tcagcggcgt gtccgatatc ggccagcgct gatgcgctcc tcacgacatg ctcccggtct
      721 gaaaattcgg gagcatgcaa tgaaggcgct tgaaggtaag gttgcaatca tcacgggcgc
      781 gagcttcggc attggccgcg ccgccgccaa attgttcgcc gccgaaggcg cacgcctcgt
      841 tctggtcgga cgacggcctg atccgctcaa tgccgtggcg gcggagattg cgagcgccgg
      901 cggcgaatgc gtggtcctcg ccggcgacgt cgcgcacgaa gatacggcgc gcgacgccgt
      961 cgcggcggcc gagcgtgctt ttggcgggtt ggacatcgcc ttcaacaacg ccggcagcct
     1021 aggtaaaatg ggacccgtaa cggacatcgc cgtctcaggc tggcgcgaag ccatcgacgc
     1081 aaacctgacc ggggccttct tcgccgcccg acatcagatt ccggcgatgc tcaaacgcgg
     1141 cggcggttcg ctcatcttca cgtccacctt cgtcgggcac acagcagggt ttccggggat
     1201 gagcgcctat gccgccagca aggccggcgt gatcggcctc atgcgcaatc tc
//
LOCUS       DASPLT010000406         1523 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_209990, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000406 DASPLT010000000
VERSION     DASPLT010000406.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1523)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1523)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1523
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_209990"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1489
                     /locus_tag="VEA63_04200"
     CDS             <1..1489
                     /locus_tag="VEA63_04200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HYD83221.1"
                     /translation="GIAQEKQKLIFEAFQQADAGTSRKYGGTGLGLSISRELAALLGG
                     EIRLNSAAGQGSTFTLYLPLFYIEPSAEKTHGALSPSASPIMLPAPREEVILDDRDQV
                     QPGDRSLLIVEDDPHYARVLLGLAREKGFKGIVATRGNAALALARQFQPTAITLDVFL
                     PDMLGWTVLNNLKLSPITRHIPVQIISVEEERSHALARGAFSYTVKPTTTEGLDECFD
                     RIKKFIEPHVKRLLVVEDNDIERQGIVELLAHDDIEIATASTGAEAMQTLLDRPFDCC
                     VLDLRLPDMSGFELLEQLQAEPSLRDIPIVVFTGKDLSRDEEQQLRTVAKSVLVKDVQ
                     SPERLFDETALFLHRVVSSLPESKQKLLDHLHASNEVLRGRKVLVVDDDARNIFALTT
                     MLENQEMNVISAMNGRQAIEMIQNQPDIAVVLMDIMMPEMDGYQTMKEIRKDSRFGTL
                     PILALTAKAMKGDRDKCLAAGASDYIAKPVNTNELLSLLRVWLYR"
BASE COUNT          294 a          403 c          546 g          280 t
ORIGIN      
        1 cggcatcgcg caggagaagc agaagctgat cttcgaggcg tttcagcagg ccgatgcggg
       61 cacgagtcgc aagtatggcg gcacgggctt gggactttcg atctcgcgcg aactggcggc
      121 gctgctgggc ggcgagatcc ggttgaacag cgcggcgggg caaggcagca cgttcacgct
      181 gtatctcccg ttgttctaca tcgagccgtc ggcggagaag acacacggcg ctttgtcgcc
      241 gtcggcgtcg ccgatcatgc tgccggcacc gcgcgaggaa gtgatcctcg acgatcgaga
      301 tcaggtgcag ccgggcgatc gcagcctgtt gatcgtggag gacgatccgc attacgcgcg
      361 cgtgttgttg gggctggcgc gggagaaagg tttcaagggc atcgtggcga cgcgtggcaa
      421 cgcggcgctt gcgctggcgc ggcagtttca gccgacggcg atcacgctcg acgtgttcct
      481 gccggacatg ctgggttgga ccgtgctgaa caatctcaag ctcagcccga tcacgcgcca
      541 cattccggtg cagatcatct cggtcgagga ggagcgttcg catgcgctgg cgcgcggggc
      601 gttttcctac acggtgaaac cgacgacgac ggaggggttg gacgagtgtt tcgaccgcat
      661 caaaaagttc atcgagccgc acgtgaagcg gctgctggtg gtcgaggaca acgacatcga
      721 gcgacagggc atcgtcgagt tgctggcgca cgacgacatc gagatcgcga cggcgtcgac
      781 gggagcggag gcgatgcaga cgctgctcga ccggccgttc gattgttgcg tgctcgattt
      841 gcggctgccg gacatgagcg ggtttgaact gctcgagcag ctacaagcgg agccgtcgtt
      901 gcgcgacatt ccgatcgtgg tgttcaccgg caaggatctc agtcgggacg aggagcagca
      961 gttgcgcacg gtggcgaaga gcgtgctggt gaaagatgtg caatcgccgg agcgattgtt
     1021 cgacgagacg gcgttgttcc tgcatcgcgt ggtgtcgagc ctcccggagt cgaagcagaa
     1081 gctgctggat cacctgcacg cgtcgaacga agtgttgcgc ggtcgaaagg tgctcgtggt
     1141 cgacgatgac gcgcggaata ttttcgcgct gacgacgatg ttggagaatc aggagatgaa
     1201 cgtgatcagc gcgatgaacg gccggcaggc gatcgagatg attcagaatc agccggacat
     1261 cgcggtcgtg ctgatggaca tcatgatgcc ggagatggac ggctatcaga cgatgaagga
     1321 gatccggaag gattcgcgtt tcggcacgct gccgattctg gcgctgacgg cgaaggcgat
     1381 gaaaggcgat cgcgacaagt gtctggcggc gggcgcctcg gactacatcg cgaagccggt
     1441 gaacacgaac gaactgctct cgctgttgcg cgtatggctg tatcggtgaa agtgggcatc
     1501 agatggtcgg tccctgcgct cac
//
LOCUS       DASPLT010000407         2932 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_44686, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000407 DASPLT010000000
VERSION     DASPLT010000407.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 2932)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2932)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2932
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_44686"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..878
                     /locus_tag="VEA63_04205"
     CDS             <1..878
                     /locus_tag="VEA63_04205"
                     /inference="COORDINATES: protein motif:HMM:NF021041.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pectate lyase"
                     /protein_id="HYD83222.1"
                     /translation="LRVWPNEDYWKYYTLNDGLPDSITNTLLTVHEVYRDERSRAALV
                     RFADFLLLAQMPDPQPAWCQQYDFNMQPTWARKFEPPSISVMESEDAVKALLRVYRLT
                     GDTRYLGAVRRALPFLKSCQLPDGRMARYYELQTNKPLYMTRPPGVSGRSSAPGYYTL
                     TYDDSDLPTHYGWKQPTEIDALMAEFAQLDRDIAPPPPALMPRFTAGGKLESVELTAP
                     PGPGGDGFAALEEQVRGILAALDSKGRWTDVAEKGRRLVGQPDFKPGFRYIGSDTFNW
                     NVQTLSDYLLATRVD"
     gene            911..2872
                     /locus_tag="VEA63_04210"
     CDS             911..2872
                     /locus_tag="VEA63_04210"
                     /inference="COORDINATES: protein motif:HMM:NF027452.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4838 domain-containing protein"
                     /protein_id="HYD83223.1"
                     /translation="MKDRLPLGAGLQLVLLIAVLHVGASGAESSADLTLVADGRAQAV
                     VVTEDEPTAVAAYAAQELVYHLEKATGAKLEIVPEKALRMDRLARIHIGNTRAAQAAD
                     IDVAQLAPEVFALRIAGTAVFIAGNDAPGDPLDSGTFAGTLFGVYEFLERELGVRWLW
                     PGELGTFVPKRRTLAVAPVDEIVSPRFMQRNVRGGLTLAGKDERLGFSHAAAEDYAKE
                     QAVFLRRHRMGRSERLFYRHAFTDWWAKYGEMHPEWFQLVNGKRGPRKPGETYSMCVS
                     NPELHREIVAQWQQRRETDPTNDPRYLNVAENGTVGLCECANCRAWDGVQPADFLDHY
                     PPESKMALTGKRFVTDRYARFWLAVQKEAEKVDPDATIVAYNYFNYFYHPSPEIKLNA
                     RILVGSYPSSGWYPRSAAEHDWFRRQWAGWQASGARLFSRGNYCLDGYAMPHIYAHQF
                     ADEFKEQVRQGMVATDYDALTGQWAAQGANLYLLMRLHTRPDAAADELLAEYYSAFGA
                     AAPQVKACFDYWERYAIEQIEQAKVLFADNTTRWRAFAKVAHRLFPETCFVPAETLLA
                     DAANAVADDPESLARVQFLQTGLTHAKLCSRVAALLSQNEPAVAAERGAQVLDELIAF
                     RRAHEREWIGNFNHSAWVEEASWRLSPEN"
BASE COUNT          546 a          851 c          956 g          579 t
ORIGIN      
        1 ggctgcgcgt gtggcccaac gaagattact ggaaatacta tacgctcaac gacggcctgc
       61 cggattcgat caccaacacg ctgctcacgg tgcacgaggt gtatcgggac gagcgttcgc
      121 gggcggcgct ggttcgtttc gccgattttc tgttgctcgc gcaaatgccc gacccgcagc
      181 cggcgtggtg tcagcaatac gatttcaaca tgcagccgac gtgggcgcgc aagttcgagc
      241 cgccgtcgat ttccgtgatg gaatccgagg acgccgtgaa ggcgctcctt cgcgtgtatc
      301 gactgactgg agacacgaga taccttggcg cggtccggcg ggcgctgccg ttcctcaaga
      361 gctgccagtt gcccgatggc cgcatggccc gctactacga gttgcagacg aacaagccgc
      421 tttacatgac ccgtccgccg ggcgtcagcg gccggtcgtc cgctcctgga tattacacgc
      481 tcacttacga cgattcggat cttccgacgc attatggctg gaaacagccg acggagattg
      541 acgcgctcat ggccgagttc gcgcaactcg atcgcgacat cgctccacct ccgcccgcgc
      601 tgatgccgcg ctttaccgcc ggcggcaaac tggagtcggt cgagctcacc gcgccgcccg
      661 ggccgggtgg agatggcttt gccgcgctcg aggaacaggt gcgcggaatt ctcgcagcgc
      721 tcgactccaa ggggcgctgg accgacgtag cggaaaaagg ccggcgcttg gtcggccagc
      781 ctgacttcaa gcccggcttc cgttacatcg gcagcgatac gttcaactgg aacgttcaaa
      841 cgctgagcga ctatctgctc gcgacgcggg tggattgagc tgcttgcgct gacgttcggc
      901 cggcttctcg atgaaagatc gtttaccgtt gggagccggt ctccagttgg tgctgcttat
      961 cgcggttctg cacgtcgggg cgagcggcgc ggaaagttcg gccgacctga cgctcgtcgc
     1021 ggatggccgc gcacaagccg tggttgtcac ggaggacgag cccacggccg tcgcggccta
     1081 tgccgcgcag gaattggtct atcatctaga aaaagccacc ggcgcgaaac tggagatcgt
     1141 gccggaaaaa gcgctgcgaa tggaccgatt ggcgcgtatc catattggca acacacgagc
     1201 ggctcaggcc gccgacatcg acgtcgcgca acttgcgccc gaggtgttcg cgctgcgaat
     1261 cgccggcacg gcggtcttta tcgcggggaa cgacgccccg ggcgatccgc tcgattccgg
     1321 cacgttcgcc ggcacgcttt ttggggtcta tgaatttcta gagcgtgagc ttggcgtccg
     1381 ctggctctgg cccggagaat tgggaacgtt cgtgccgaaa cgacgaacgc tcgcggtcgc
     1441 acccgtggac gaaatcgttt ccccgcgttt catgcagcgc aatgtgcgcg gtgggctgac
     1501 gctcgcgggc aaagatgaga ggctgggatt ctcgcatgcg gcggctgaag actacgcgaa
     1561 ggaacaggcg gtgtttcttc ggcgccatcg gatgggtcgg tcggagcggc tcttttatcg
     1621 gcatgcgttc accgactggt gggcgaaata cggcgagatg catcccgagt ggtttcagtt
     1681 ggtgaacgga aaacgcggac cgagaaagcc gggcgaaacg tattcgatgt gcgtttcaaa
     1741 tccggagctt catcgtgaga tcgtcgcgca gtggcagcag cggcgcgaaa ccgatccgac
     1801 aaacgatccg cgctacttga acgtcgccga gaacggcacg gtgggtttgt gcgagtgtgc
     1861 gaattgccgt gcgtgggacg gagtgcaacc ggcggacttt ctcgatcatt atccgccgga
     1921 gtcgaaaatg gcgctcacag gaaagcggtt tgtgacggat cgctatgcac gattctggct
     1981 cgccgtgcag aaagaggccg agaaggtcga tccggatgcg acgatcgtcg cctacaacta
     2041 cttcaactat ttttatcatc cctcgccgga gataaaactg aacgcgcgca tcctcgtcgg
     2101 ctcgtatccg tcttcggggt ggtatccgcg ttcggcggcg gagcacgact ggtttcgccg
     2161 acaatgggca ggctggcagg cctcgggcgc gcggcttttt tcgcggggca attactgcct
     2221 cgatggctat gcgatgccgc acatttacgc gcatcagttt gcggacgagt tcaaagagca
     2281 ggtgcgtcag ggaatggtcg cgaccgatta cgatgcgttg acgggacaat gggcggcgca
     2341 gggtgcgaat ctctatctgc tgatgcggtt gcacacgcgc ccggacgcag cggcggacga
     2401 gttgctcgcc gaatattact cggcctttgg ggccgcggcg ccgcaggtga aagcctgctt
     2461 cgactactgg gagcgttacg cgatcgagca aatcgaacag gcgaaggtgc tcttcgcgga
     2521 taacacgacg cgctggcgtg cattcgccaa agtcgcgcat cggttgtttc cggaaacatg
     2581 ttttgttccg gctgaaacct tgctcgcaga cgccgcgaat gcagtcgcgg atgaccccga
     2641 gtcgctggcg cgcgtgcagt ttttgcagac tggtttgacg catgcgaagc tctgctcgcg
     2701 cgtcgccgcg ctgctttcgc aaaacgagcc cgccgtcgcg gctgagcgag gcgcgcaggt
     2761 gctcgacgaa ctgatcgcgt tccggcgcgc gcacgaacgg gagtggatcg gcaactttaa
     2821 tcacagcgcc tgggtggaag aagccagctg gcgcctttcg ccagaaaact aagtttcaaa
     2881 ggaggcgcat gtttactcga aattttcgcc gcttggttcg tttcaccgct gc
//
LOCUS       DASPLT010000408         1144 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_127580, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000408 DASPLT010000000
VERSION     DASPLT010000408.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1144)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1144)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1144
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_127580"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            339..>1144
                     /locus_tag="VEA63_04215"
     CDS             339..>1144
                     /locus_tag="VEA63_04215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD83224.1"
                     /translation="MLFPFRIRAAAVAAGLFFCLFTSVRAVEIEVEEGAPTPVHGVKD
                     PGTRVAEAEIAPASDEAEQALERMKLPAGLKASLWAAEPMLANPVAFNIDEQGRIFVA
                     ETYRYRSSVLDIRDYLWMLEDDLATRTIEDREKLVAEKLGAEGTKELSIESERLRLIE
                     DRDGDGKADHSSLYAEGFNSVLDGIASGVVARRGEVWFTNIPSLWKFTGRDKAETREE
                     LSRGYGVRFNYTGHDFHGPTFGPDGKLYFSCGDRGTHVTTKEGRIISVPDT"
BASE COUNT          215 a          345 c          381 g          203 t
ORIGIN      
        1 atccgggtgt gatcgacacg gacatgttgc gctcgtgctg ggcggaaggc gcggcgagtt
       61 atccgaaggc ggaggcgtgg gcgaaagcgg cggcgccgtt catcctgaaa atcggcgcca
      121 agcaaaacgg acgttcgctg acggtgggcg gcttcgagga gcaggaatag cgggcaagcc
      181 ggtttgcgga agttcaggcg acttgcattc tgggcgcggc tcggctcggc aggtgaccac
      241 gtcgcgcctc cgccttccga ctgcacgtcg agaaatcttg ggcgaggacg ccacccttgc
      301 caccttggca cgtcttacta aactctctcg tttcccccat gctgtttcct ttccgcatcc
      361 gcgcggcagc ggtcgctgcc ggcctttttt tctgtctctt caccagcgtt cgcgcggtgg
      421 agatcgaggt ggaggagggc gcgccaacgc cggtgcatgg cgtgaaggac cccggcactc
      481 gcgtggccga ggcggaaatc gctcccgcat cggatgaagc ggagcaggcg ctcgagcgca
      541 tgaaacttcc cgcgggcctg aaggcttccc tgtgggcggc ggagccgatg cttgcgaacc
      601 ccgtggcgtt caacatcgac gaacaggggc gcattttcgt tgccgagaca tatcgctacc
      661 gctcgagcgt gctcgatatc cgcgactacc tttggatgct cgaagacgac ctcgccacgc
      721 ggacaatcga agaccgtgag aaactcgtgg cggaaaaact cggcgccgag gggacgaagg
      781 aactttccat tgagagcgag cggctgaggc tgatcgagga tcgtgatggc gacggcaagg
      841 cggatcactc cagcctctac gcggaaggat tcaacagcgt gctcgatggc atcgcgtcgg
      901 gcgtggttgc gcgtcgcggc gaggtgtggt tcaccaacat cccgagcctt tggaaattca
      961 ccggccgcga caaggccgag acgcgtgaag agctcagccg cggctatggc gtgcggttca
     1021 actacaccgg tcacgatttt cacggcccga cgttcgggcc cgacgggaaa ctctatttca
     1081 gctgcggcga ccgcggcacg cacgtcacga cgaaagaagg ccgcatcatc tccgtgcccg
     1141 acac
//
LOCUS       DASPLT010000409         1139 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_127582, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000409 DASPLT010000000
VERSION     DASPLT010000409.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1139)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1139)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1139
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_127582"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..209)
                     /locus_tag="VEA63_04220"
     CDS             complement(<1..209)
                     /locus_tag="VEA63_04220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD83225.1"
                     /translation="MNFRLLPLASLTLVLAPLMPAQVSRNPEEPVPVHRAPPKTDHQS
                     LLTPPGGEDQKVYGPRYTSLVDGEAA"
     gene            complement(272..694)
                     /locus_tag="VEA63_04225"
     CDS             complement(272..694)
                     /locus_tag="VEA63_04225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcfL family protein"
                     /protein_id="HYD83226.1"
                     /translation="MNITHRFPVFAGVAALLLLAGCATEPGPFAPQDTTKYTIENTEK
                     FVLLDKPTQYSITCTGLQEYTLPDGRLEVVANVKNRENRRLQVQINCVFKDEQGFTTG
                     DETPFQNLILAEYSTEAVRFTAGNTQAKRYTIRVRQAR"
     gene            complement(729..>1139)
                     /locus_tag="VEA63_04230"
     CDS             complement(729..>1139)
                     /locus_tag="VEA63_04230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD83227.1"
                     /translation="RVMDHILGNKAVFKSTTNTIGDVAAIGSIAAASRINRYDGTHSE
                     GSQNTALALGAVALISKIASAATTPDADTRTWNNLPQRLSFAALRLAPGQYPAKVQFF
                     DAAGTLLAGLTKELTVVVDDDARDTVVFLSELNR"
BASE COUNT          204 a          368 c          342 g          225 t
ORIGIN      
        1 gccgcctcgc catccaccaa cgaggtgtaa cgcggcccgt aaactttctg gtcctcgccc
       61 cccggcggcg tgagcagcga ttgatgatcc gtcttcggcg gcgcccgatg caccggcacc
      121 ggctcctccg gattgcgcga cacctgcgcc ggcatcagcg gtgcgagaac cagcgtgagc
      181 gacgcgagag gcagaaggcg gaagttcatg gcttggggtt ggattgagcc gacgcccgca
      241 cccgcgcacg agcagcagcc tcggagttta tctagcgagc ttgtcgaacg cgaatcgtat
      301 aacgcttcgc ctgcgtattc cccgcggtga aacgcaccgc ttcggtcgaa tattcggcga
      361 gaatcaaatt ctgaaacggc gtttcgtcgc cggtcgtgaa gccctgctcg tccttgaaca
      421 cacagttgat ctgaacctgc aatcgccggt tctcgcggtt cttcacgttc gccacgactt
      481 cgagacggcc atccggcaac gtgtattcct gcagccccgt gcacgtgatc gaatactgcg
      541 tcggtttgtc gagcagcacg aacttctccg tgttttcgat cgtgtatttc gtcgtatcct
      601 gcggcgcgaa cggaccgggt tccgtcgcgc agccggcgag taaaagcagc gcggcgacgc
      661 cggcgaaaac agggaaacgg tgcgtgatgt tcatagtcgt tgtgtgagtg aaggggtgag
      721 actgccggtc agcggtttag ttcactgaga aaaaccaccg tatctcgcgc gtcatcatcg
      781 acaacaaccg tcagctcctt cgtcaacccc gcgagcagcg ttcccgccgc atcgaaaaac
      841 tgcaccttcg ccggatattg cccgggcgcg agccgcagcg ccgcgaaact cagccgctgc
      901 ggaaggttgt tccacgtgcg cgtgtcggcg tccggcgtcg tcgccgccga cgcgatcttg
      961 ctgatcaacg ccaccgcccc gagcgccagc gccgtgttct gcgagccttc gctgtgcgtg
     1021 ccgtcatagc gattgatgcg actcgccgcc gcgatcgaac cgatcgccgc gacgtcgccg
     1081 atcgtgttcg tcgtgctctt gaacaccgcc ttgttgccga ggatgtgatc catcacgcg
//
LOCUS       DASPLT010000410         1331 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_189648, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000410 DASPLT010000000
VERSION     DASPLT010000410.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1331)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1331)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1331
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_189648"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..747
                     /locus_tag="VEA63_04235"
     CDS             <1..747
                     /locus_tag="VEA63_04235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015440 - ABC-type peptide transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide/dipeptide ABC transporter
                     ATP-binding protein"
                     /protein_id="HYD83228.1"
                     /translation="QSSAAEIRDARRDLQMVFQDPYASLNPRMTVFDTLAEPLLLHHR
                     CASGELTSRVTELMRQVGLAPRFMRKYPHEFSGGQRQRIAIARALALDPKVIIADEPV
                     SALDVSIQAQILNLLAQLGRERNLSLVFIAHDLSVVKHISDRIAVMYLGKIVELGPSV
                     DVIEHPAHPYTRALVSAIPTPNPDVERNRQRIVLAGDPPSPIDPPVGCRFHPRCPYAV
                     EKCRAIVPPLLPAGPAREAACIRLSEIPPT"
     gene            complement(829..1296)
                     /locus_tag="VEA63_04240"
     CDS             complement(829..1296)
                     /locus_tag="VEA63_04240"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007362691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008175 - tRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (cytidine(34)-2'-O)-methyltransferase"
                     /protein_id="HYD83229.1"
                     /translation="MIHVVLFQPEIAPNTGNIGRMCALTRSRLHLIHPLGYVINDRNL
                     RRAGMDYWKALDVHEHADWAAFRASAVAPKRLWLFTTHAAQSFWDVRYEEGDGLVFGN
                     ESSGAPVWLHEEIGAAQRVTIPHANPELRSLNLSTAAGVACYEALRQVGTLRG"
BASE COUNT          241 a          530 c          331 g          229 t
ORIGIN      
        1 cagagctccg ccgccgaaat tcgcgacgcc cgccgcgatc tccagatggt gtttcaggat
       61 ccttacgcgt cactgaatcc gcgtatgacc gttttcgata cgctggccga gccgctcctc
      121 cttcaccacc gctgcgcgtc cggcgaactc acctcgcgcg taaccgagct catgcgccag
      181 gtcggcctcg cgccgcgctt catgcgcaaa tacccgcacg aattctccgg cggccaacgc
      241 cagcggatcg ccatcgcccg cgccctcgcg ctcgacccaa aagtcatcat cgccgacgaa
      301 cccgtctccg ccctcgatgt gtcgatccag gcgcagatcc tcaatctgct cgcgcaactc
      361 ggccgggaac gaaatctctc gctcgttttc atcgcgcacg acctttccgt cgtgaaacac
      421 atttccgatc gcatcgccgt gatgtatctc ggaaaaatcg tcgagctcgg cccctcggtc
      481 gacgtgatcg agcaccccgc gcatccctac acgcgcgcgc tcgtcagcgc gattcccacg
      541 ccgaatcccg acgtcgaacg caaccgccag cgcatcgtgc tcgccggcga tccaccttca
      601 ccgatcgatc cgcccgtcgg ctgccgcttt catccgcgtt gcccttacgc cgtcgaaaaa
      661 tgccgggcca tcgttccgcc cctgctcccc gccggccccg cccgcgaagc cgcctgcatc
      721 cgcctcagcg aaatccctcc gacctgatcc cgttgggcgg acttcagtcc accttcacgc
      781 gctggagcca agccgagcgg gatgaacgcc cgtcccaccc gccgctcctc agccgcgcag
      841 cgtccccacc tgccgcaacg cctcatagca cgccacaccc gccgccgtgc tgagattcag
      901 cgagcgcaac tccggattcg cgtgcggaat cgtcacgcgt tgcgccgccc cgatctcctc
      961 gtgcaaccac accggcgcgc ccgaactctc gttcccaaaa acgagcccgt cgccctcctc
     1021 gtaacgcacg tcccaaaagc tctgtgccgc atgcgtcgta aatagccaca gccgcttcgg
     1081 ggccaccgca ctcgcccgaa acgccgccca atccgcgtgc tcatgcacgt cgagcgcctt
     1141 ccaataatcc atgccggcac ggcgcagatt ccggtcgttg atcacatagc cgagcgggtg
     1201 aatcaggtgc agccgcgatc gggtcaacgc gcacatccgg ccgatattgc ccgtattcgg
     1261 agcgatctcg ggttgaaaca gcaccacgtg aatcatcgct ccatctcgct aacccccggt
     1321 taaaaactca a
//
LOCUS       DASPLT010000411         1258 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_106044, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000411 DASPLT010000000
VERSION     DASPLT010000411.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1258)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1258)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1258
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_106044"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1258)
                     /locus_tag="VEA63_04245"
     CDS             complement(<1..>1258)
                     /locus_tag="VEA63_04245"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HYD83230.1"
                     /translation="EVSSPKFTAPLVDTPQTISIIPKEVFNQQGAANLADVLGNTPGI
                     TFLAGEGGHVSGSNSFVMRGFDVSGNIFIDGVRDNGNYGRDIYNLDQVEVVKGPAGDN
                     GRGAASGYLNLVTKTPQAENFGSITGSYGFDEYASIERKRTTLDVNQSLDSVQSGAAF
                     RLNALWQDGGVAGRDYAEKNTWGIAPSLAFGLNSATRVIATYQHDEQHDRPDYGALAG
                     TISETATAVRPAAPIDRSRYYGLLSDYDDVTIDVASLRLEHDLTPTMRLTNFTRYSNT
                     DREAIYAVPSSVDNRPDMNTGERTEIVVTNRQAFAREIETLVNQTNLASQFSTGSFDH
                     SFAGGLELIRENGRTLRDWTGLGTVGELSKMVNVTTAGAPTTPTLVVGTNPYHPNPAR
                     PITGFAPTYRFIDDIRIDTVALYAYDT"
BASE COUNT          197 a          312 c          496 g          253 t
ORIGIN      
        1 ggtgtcgtag gcgtagagcg cgacggtatc gatgcggatg tcgtcgatga atcgatacgt
       61 cggggcaaag ccggtgatcg ggcgggcggg gttggggtgg taaggattgg tgccgacgac
      121 gagtgtgggc gtggtgggcg cgccggcggt ggtgacgttc accatcttgc tgagttcgcc
      181 gacggtgccg aggccggtcc aatcgcggag cgtgcggccg ttttcgcgga tgagttcgag
      241 gccgccggcg aaggagtgat cgaacgagcc ggtggagaat tgcgaggcga ggttcgtctg
      301 gttgacgagg gtttcgatct cgcgggcgaa ggcctggcgg ttggtcacga cgatttccgt
      361 gcgctcgccg gtgttcatgt cgggacggtt gtcgacggaa ctcgggacgg cgtagatggc
      421 ttcgcggtcg gtgttgctgt agcgcgtgaa attcgtgagc cgcatcgtcg gcgtgaggtc
      481 gtgctcgagc cggaggctgg cgacgtcgat cgtgacatcg tcgtaatccg agagcaggcc
      541 gtagtagcgg ctgcgatcga tcggtgcggc gggacgaacg gcagtggcgg tctcggaaat
      601 cgtgccggcg agcgcgccgt agtcggggcg gtcgtgttgt tcgtcgtgct ggtaagtggc
      661 gatgacgcgt gtcgcggagt tgaggccgaa cgcgagcgag ggagcgatgc cccaggtgtt
      721 tttctcggcg tagtcgcggc cggcgacgcc gccatcctgc cacagcgcgt tgaggcgaaa
      781 cgcggcgccc gattgcacgg aatcgagcga ttggttaacg tcgagcgtgg tgcgtttgcg
      841 ttcgatcgaa gcgtattcgt cgaagccgta gctgccggtg atactgccga agttttccgc
      901 ctgcggcgtc ttcgtgacga gattgagata accggacgcg gcgccgcggc cgttatcgcc
      961 ggcgggacct ttgacgacct cgacctggtc gaggttgtag atgtcgcggc cgtagttgcc
     1021 gttgtcgcgg acgccgtcga tgaagatgtt tccggagacg tcgaagccgc gcatcacgaa
     1081 gctattcgag ccggaaacgt gaccgccttc gccggcgagg aaggtgatgc ccggagtgtt
     1141 gccaaggaca tcggcgagat tggcggcgcc ttgctggttg aagacctctt tggggatgat
     1201 ggaaatggtc tgcggggtgt cgacgagcgg cgccgtgaat ttgggcgagg agacctcg
//
LOCUS       DASPLT010000412         4476 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_292263, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000412 DASPLT010000000
VERSION     DASPLT010000412.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 4476)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4476)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4476
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_292263"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1842
                     /locus_tag="VEA63_04250"
     CDS             <1..1842
                     /locus_tag="VEA63_04250"
                     /inference="COORDINATES: protein motif:HMM:NF039835.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HYD83231.1"
                     /translation="LGAVDDADINRMSGGYVTTVNLKTAAHVQLRLSYDLSVASAFES
                     EERFEVLASVDGVLKGLNGQDYIQRTNGPFLHAGRVSVPLGRLSAGPHRIAVGGYLSK
                     KTEKAELATLYLDWLSVETLPAENALPTVNITSPVEGAHYPPREGPEFIDVAVVDSDG
                     SIERVELHSSERFISPIRILTEPPYRFPGYNTSGFNRLFVRAFDNRGAVGVDAVTFVV
                     GAVENQSPRGDYTSPVPGQFVKLGSTLRVSGVASDADGTIERVEVYLEDVLQGTDYTA
                     PYSVDVPLAGLPEGAYGLRLVPWDNEGGANGAIETYFQIVANLPPVVTMTSPLPDTIF
                     NKGTTVRVRVAAVDADGTVARVELEVDGGQIRLTDTSAPYEFLVAGLAPGDHFFRVRA
                     IDNEGRPSDEPADAYVQLGVIVAAGNGQVTPPSGGGGFYYNPGEIVTFTANAAPAGYV
                     FDQWIGETDFLANPFAATTTLKVPTGQTSRSPTATYKPATNPTMLRFVLINADSDLPI
                     AGYDPLYNGAVLNLSKLATRNLNIRAVTTPEPTGSVHFALDAGASAAKQNVENVAPYA
                     FALDDSGNYRRWTPSAGKHTLTAWAYTAKNKSGTRSQPLTINFNVVP"
     gene            complement(2030..2305)
                     /locus_tag="VEA63_04255"
     CDS             complement(2030..2305)
                     /locus_tag="VEA63_04255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD83232.1"
                     /translation="MKRIAPLQLGKMSALLYGAMGLLFLPVFLIMAVASAAAPEAQQI
                     GIFAFGAGFALLAPVMYAVMGFIVGVIGAALYNLFAKWVGGIEVEVE"
     gene            2645..3025
                     /locus_tag="VEA63_04260"
     CDS             2645..3025
                     /locus_tag="VEA63_04260"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HYD83233.1"
                     /translation="MSYSLLLVDDQDTVLRVLEYVFTHKGHRVLTANSGKAALELLRK
                     EPVDAAVIDMHMPEMDGFQTAEALLTLARERGDRLRVWMMTGAFSTAAQKRAQEIGAV
                     ALLAKPFDYDRLCVDLARELGSRD"
     gene            complement(3048..>4476)
                     /locus_tag="VEA63_04265"
     CDS             complement(3048..>4476)
                     /locus_tag="VEA63_04265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HYD83234.1"
                     /translation="IMLLLGWVFVPFYHRSGVFTMPEFLERRFNARTRWALSIVSLIA
                     YVFTKVSVTVYAGAIVFQALLPDTFGSPENAFWVGAITTVVLTGVYTIFGGLRAVVYT
                     EVAQTILLLGGSAFVTIFALGKLGGWSELKATLAANPEQFALWRPLSDPNFPWLGVMI
                     ASPIIGIWYWCTDQYIVQRTLAARDLTQARRGAIFGGFLKVLPVFLFLIPGMIGYALY
                     QKGLLPLATNADGSVRGDAVFPTMVSTLLPAGLRGIVVAGLLSALMSSLASLFNSCAT
                     LFTVDIYGKLRPGRPDRELVKVGRIATGVVVLLGILWIPVMPLVSKGGLYEYLQAVQG
                     YLAPPITAVFLLGLFVRRINARGAFAGLVIGFALGVLKLGLQAAAGSGALGDSGVLFE
                     FGQINFLYYSGMLFLVSVLVVIFASLTAPAPDEAQVVGLTYRSVTAAQHEEVRRSWGW
                     PDVLGTLLVLGLVLGIYLYFSFWLS"
BASE COUNT          894 a         1280 c         1545 g          757 t
ORIGIN      
        1 ctcggcgccg tcgacgatgc ggatatcaac cgcatgtcgg gcggctacgt caccacggtg
       61 aatttgaaaa ccgcggcgca cgtgcaactg cggctctctt acgatttgag cgtcgcgagc
      121 gcgttcgaga gcgaggagcg gttcgaggtg ctggcttccg tcgacggcgt gttgaagggt
      181 ctcaacggtc aggattacat tcagcgcacc aacggcccgt ttcttcacgc cgggcgcgtt
      241 tcggtgccgc tcggccggtt gtcggccgga ccgcaccgca tcgcggtcgg cggttacttg
      301 agcaagaaaa cggagaaggc cgagctcgcc acgctctatc tcgattggct gtccgttgaa
      361 accctgccag cggagaacgc gctgccgacg gtgaacatca cgtcgccggt cgagggggcg
      421 cactatccgc cgcgtgaggg gccggagttc atcgacgtcg cggtggtcga cagcgacggc
      481 tccatcgagc gggtggaact gcattcgagc gaacggttca tcagcccgat ccgcattctc
      541 acggagccgc cgtatcgatt tcctggttac aacaccagcg gcttcaatcg actgttcgtt
      601 cgggcattcg acaatcgagg cgccgtcggc gtggacgcgg tgacgtttgt ggtcggggcg
      661 gtggagaatc aatcgccgcg gggggactac acgtcgccgg tgccgggcca gttcgttaaa
      721 ctgggcagca ccttgcgggt ttcgggcgtg gcctcggatg cggacggcac gatcgagcgc
      781 gtcgaagttt atctcgagga cgtgctgcaa ggcacggatt acaccgcgcc gtattccgtc
      841 gatgtcccgc tggcaggcct gcccgagggc gcttacgggc tgcggctggt gccgtgggac
      901 aacgagggcg gggcgaacgg cgcgatcgaa acttattttc agattgtcgc gaacctgccg
      961 ccggtggtga cgatgacgag cccgctaccc gacacgattt tcaacaaagg aacgacggtg
     1021 cgcgtgcgcg tcgcggcggt cgatgcggac ggcaccgtgg ccagggtgga actcgaggtg
     1081 gacggcggac aaatccgttt aacggatacg tccgctcctt atgagttctt ggtcgcggga
     1141 ctcgcgccgg gagatcattt ttttcgcgtg cgcgccatcg ataatgaagg ccgtccgtca
     1201 gacgaaccgg cggacgccta cgtgcaactc ggtgtgatcg tcgccgcggg caacggacag
     1261 gtgacgccac cgagcggcgg cggtggtttt tattataatc cgggcgagat cgtgaccttc
     1321 acggcgaacg ccgcgccggc gggctatgtg ttcgaccaat ggatcggcga aaccgatttt
     1381 ctggcgaatc ccttcgccgc gacgaccacc ttgaaagtcc cgaccggtca gaccagccga
     1441 tctccgactg cgacgtataa gccggcgacg aatccgacga tgctgcgatt cgtgctgatc
     1501 aacgcggaca gcgatttacc gatcgccggc tacgatccgc tttacaacgg cgcggtgctg
     1561 aacctgtcga aattggcgac gcgcaacttg aacatccgtg cggtgacgac gccggagccg
     1621 acgggctcgg tgcatttcgc gttggatgcg ggagcgagcg cggcgaaaca gaatgtcgaa
     1681 aacgtcgcgc cgtatgcctt cgcgttggac gactccggaa actatcgtcg ttggacgcct
     1741 agcgcgggca agcacacgct caccgcgtgg gcctacaccg cgaagaataa aagcggcacg
     1801 cgctcgcagc cgttgacgat caacttcaac gtggtgccgt gatggtgaca gcgacagcgg
     1861 ttcaaaaact ctccagccga atcgttggcc gcaaagaggc gcaaaaggcg caagaactga
     1921 agcgtcaccc aagtcagttt tgcgccttct gcgctttttt gcggctatcg gttcattgaa
     1981 ctcgctacag cgagcagagt tcaattgctg cggctctgcg gcgatggcgt cattccacct
     2041 cgacctcgat gccgccgacc cacttcgcga aaagattata gagcgccgcg ccgatcacac
     2101 cgacgataaa acccatcaca gcatacatga cgggcgccag cagcgcgaaa ccggcgccga
     2161 acgcgaatat gccgatttgc tgggcttccg gcgcggctgc gctggcgacc gccataatga
     2221 ggaagactgg caggaagagc aggcccatgg ctccgtagag gagcgcgctc attttgccga
     2281 gttgcagtgg ggcgatgcgt ttcacccggc gcttgtgcgg acctgacggc ggcgggttga
     2341 gaggagtcga tgacagggtc ggtgggattt ggtcggggga gctcatgggg aaggccgggg
     2401 atttttagcg gggtaatggc gggcgctcac cgagcgagcg ctggccggcg gggtttcgag
     2461 gccgggcaaa aggtgcgcaa agaacacaag cgcatcgcgt gcgctttcgg aagcctgaag
     2521 cacgtattat agaagaacca cccgcacggc ggcgggaatt ttgttcgtgt gtcggcaaag
     2581 cagttggtgc tccttttcag gtgccatccg ctggcggtgg cttccagttc ctccttcaac
     2641 tccgatgtcg tattccctgt tgctcgtcga cgatcaggac acggtgctcc gtgtgctcga
     2701 gtatgtgttc acgcacaaag gacatcgcgt gctcacggcg aattccggga aagctgcgct
     2761 ggagttgctg cgcaaggagc cggtcgacgc ggcggtgatc gacatgcaca tgccggagat
     2821 ggacggcttt caaacggcgg aagcgttgct cacgctggcg cgcgaacgcg gcgaccggct
     2881 gcgcgtttgg atgatgacgg gcgcgttttc gacggcggcg caaaaacgcg cgcaagagat
     2941 cggcgcggtc gcactgctgg cgaagccgtt cgactacgat cggttgtgcg tggatctcgc
     3001 gcgggagttg ggctcgcggg attgaaatcc cggcgagcgc ggcggcgtca actcagccag
     3061 aagctgaaat aaagatagat gccgaggacg aggccgagca cgagcagcgt gccgagcacg
     3121 tccggccagc cccaactccg gcggacttct tcgtgctgcg cggcggtcac cgagcgatac
     3181 gtgagtccga cgacctgcgc ttcgtccggc gcgggcgcgg tgagcgaagc gaagatgacg
     3241 acgagcacgc tcacgaggaa gagcatgccg gagtagtaga ggaaattgat ctggccgaat
     3301 tcgaacaaca cgccgctgtc gccgagtgcg ccgctgccgg cggccgcttg caggccgagt
     3361 ttcaacacgc cgagcgcaaa gccgatcaca agaccggcga acgcaccgcg ggcgttgatg
     3421 cggcgcacga agaggccgag cagaaacacg gcggtgatcg gcggagcgag gtagccttgg
     3481 acggcttgca ggtattcgta gaggccgcct ttggagacga gcggcatcac gggaatccac
     3541 aggatgccga ggagcacgac gacgccggtc gcgatgcggc cgactttcac gagctcgcga
     3601 tcgggccggc cggggcggag tttgccgtag atgtcgaccg tgaagagcgt ggcgcaggaa
     3661 ttgaagagcg aagcgagtga actcatcagc gcggacaaca agccggcgac gacgatgccg
     3721 cgcagaccgg cggggagcag cgtcgacacc atcgtcggaa acacggcgtc gccgcgcacg
     3781 gaaccgtcgg cattggtggc gagcggcagc aggccctttt gataaagcgc gtagccgatc
     3841 atgccgggta tcaggaacaa gaatacgggc agcaccttga gaaagccgcc gaaaatcgcg
     3901 ccgcggcggg cttgcgtgag gtcgcgcgcg gcgagcgtgc gttgaacgat gtattggtcg
     3961 gtgcaccaat accagatgcc gatgatgggc gaggcgatca tgacgccgag ccatgggaaa
     4021 ttcggatcgg agagcggccg ccagagcgcg aattgctcgg gattggcggc gagcgtcgct
     4081 ttgagctcgc tccagccgcc gagtttgccg agggcgaaga tcgtgacgaa agcggagccg
     4141 ccgaggagga ggatcgtttg cgcgacctcc gtgtaaacga ccgcgcggag cccgccgaaa
     4201 atggtgtaga cgccggtgag cacgacggtg gtgatcgcac cgacccagaa ggcgttttcc
     4261 ggcgaaccga acgtgtcggg cagcagcgcc tggaaaacga tggcgccggc gtagacggtt
     4321 acggagacct tcgtgaacac ataggcgatg agcgagacga tggacagcgc ccaacgcgtg
     4381 cgggcgttga agcggcgctc gaggaactcg ggcatcgtga atacgccgga gcgatggtag
     4441 aacggcacga acacccagcc gaggagcagc atgatc
//
LOCUS       DASPLT010000413         2133 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_87106, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000413 DASPLT010000000
VERSION     DASPLT010000413.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 2133)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2133)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2133
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_87106"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..425
                     /locus_tag="VEA63_04270"
     CDS             <1..425
                     /locus_tag="VEA63_04270"
                     /inference="COORDINATES: protein motif:HMM:NF024299.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HYD83235.1"
                     /translation="RIQPDLAAAHRNLGLAIATAGRHAEAIPHFERAIALAPHDRDAE
                     LSLAIALMATDRFTAAVPHFERAIALSPHSPNAHHTYARALASRGDLDAAIRHLRDAV
                     ALAPQRPDLHRDLALALRQAGRLEEAQHHFLQAGASTR"
     gene            946..1737
                     /locus_tag="VEA63_04275"
     CDS             946..1737
                     /locus_tag="VEA63_04275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007360646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HYD83236.1"
                     /translation="MNNTSDFLQQFFGLSGKVAVVIGGTGELCGAMAEGLAGAGAEVV
                     IVGRNAEKAEARLKKISAAGGKAWFHSAEATSKSEVEVLLAAVLERSGRVDILVNGAG
                     INSATPFFDIGEEEFDRILRVNVKGVFLGCQVFGKYLVERGQGGSIINLGSMSGIVPL
                     SRVITYSASKGAVHNLSLNLAREWAPAKVRVNVLVPGFFPAEQNRKILSPDRVSAILG
                     HTPMKRFGEARELIGATLLLASDAAGSFITGEELIVDGGYHAMTI"
     gene            1871..>2133
                     /locus_tag="VEA63_04280"
     CDS             1871..>2133
                     /locus_tag="VEA63_04280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012377091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD83237.1"
                     /translation="MKPINAFLLRHNLRIAQNPCVSPDFCLDWDALSGKIVAGAPLKV
                     WPKSRFETAVGTWTLLEDEATGDCAWKLETRATGASAGELHRGL"
BASE COUNT          386 a          835 c          529 g          383 t
ORIGIN      
        1 tccggataca gcccgacctc gccgccgccc atcgcaacct cggcctcgcc atcgccaccg
       61 ccggacgcca cgccgaagcc atcccgcatt tcgaacgcgc catcgcgctc gcgccgcacg
      121 atcgcgacgc cgaactcagc ctcgccatcg cactgatggc caccgaccgc ttcaccgccg
      181 ccgtcccgca tttcgaacgc gccatcgccc tcagccccca ctcaccgaac gcgcaccaca
      241 cttacgcccg cgccctcgcc agccgcggtg acctcgacgc cgccatccgt catcttcgcg
      301 acgccgtcgc cctcgctccg cagcgtcccg acttgcaccg tgatctcgcc ctcgccctcc
      361 gccaagcggg ccgcctcgaa gaagcgcaac accacttcct ccaagctgga gcctcaaccc
      421 gctagagcag atcgattcaa acgatagccg caaaaaagca cccaagtcgc aaaatgactt
      481 gaaggctcct cgccttgcgg cttctgcgcc tctctaccgc cagcgatttg gctacagatt
      541 tttttgaacc gctgtaggta aagcccgaca tctccgccgg ctgtttgctc ctcgcgtttg
      601 tttgtcgcat cgcccgcgcg cgccaaaccc actcgctaca ctcaacgatg agcgcacgcc
      661 gcgattcgga aacggccgcc gctgccgcca ccttctcttc cgcgtcactc ggacgcatcg
      721 ccgcaggccc ctcgccgccg gccggatgcg cgttcagatc cacgcggccg ctgctccggc
      781 tctgcgccgg cccatgtttt cttgttcctt cacgattctt ccgctcccgc ggcgccacag
      841 aaaaatcacg ctcatcatca tcattttccc ttcacgcact ccgccctttg atcgtctaca
      901 taatgcgctc gtctggcgtc gttcccccgc caaacgtgtt cttcgatgaa taatacctcc
      961 gacttcctcc aacagttctt cggcctgagc ggcaaagtcg ctgtcgtcat cggcggcacc
     1021 ggcgaactct gcggcgcgat ggccgaaggc ctcgccggcg ccggcgccga agtcgtgatc
     1081 gtcggccgca acgccgagaa agccgaggcg cgcctaaaaa aaatctccgc cgccggaggc
     1141 aaggcctggt tccactccgc cgaagccacg agcaaaagcg aagtcgaagt tctccttgcc
     1201 gccgtgctcg agcgctcggg ccgcgtcgac atcctcgtca acggcgccgg catcaactcc
     1261 gccactccgt tcttcgacat cggcgaggag gagttcgacc gcatcctccg cgtcaacgtc
     1321 aagggtgtct tcctcggctg ccaggtcttc ggcaaatacc tcgtcgaacg cggccagggc
     1381 ggctcgatca tcaatctcgg ctcgatgtcc ggcatcgtcc cgctctcacg cgtcatcacc
     1441 tactccgcct ccaaaggcgc cgtccacaat ctctcgctca acctcgcccg cgaatgggca
     1501 cccgccaagg tccgcgtcaa cgtcctcgtc cccggcttct tccccgccga gcaaaaccgc
     1561 aagatcctct cgcccgatcg cgtatccgcg attctgggac acacgccgat gaaacgcttc
     1621 ggcgaagccc gcgagctgat cggcgccacc ctcctcctcg cctccgacgc cgccggctcc
     1681 ttcatcaccg gcgaagaact catcgtggac ggcggctatc acgcgatgac catctgaacg
     1741 caatccgcag agaaccacga atgcacacga atcgacacga ataccggaaa agccgcgcgc
     1801 tcgtgagctc tgccttcatt cgtgtccctt cgtgtccatt cgtggttaaa accatttcca
     1861 ctttccctct ttgaaaccga tcaacgcctt cctcctccgc cataacctgc gcatcgcgca
     1921 gaatccgtgc gtcagcccgg acttctgcct cgattgggac gccctgagcg gaaagatcgt
     1981 cgccggcgcg ccgctgaaag tctggcccaa gagccgcttc gaaaccgccg tcggcacgtg
     2041 gacgctactc gaagacgaag ccaccggcga ctgtgcttgg aaattggaga cgcgcgctac
     2101 cggcgcctcc gccggcgaac tccatcgcgg tct
//
LOCUS       DASPLT010000414         1225 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_271198, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000414 DASPLT010000000
VERSION     DASPLT010000414.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1225)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1225)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1225
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_271198"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..694)
                     /gene="hisN"
                     /locus_tag="VEA63_04285"
     CDS             complement(<1..694)
                     /gene="hisN"
                     /locus_tag="VEA63_04285"
                     /EC_number="3.1.3.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007363071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004401 - histidinol-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase"
                     /protein_id="HYD83238.1"
                     /translation="MDLALYRSFVAQLAEKSGDFIRPFFGDHAVAVDVKSDASPVTAA
                     DRGAEKLLRSLIAEAFPTHGIIGEEFGSENADAEFVWVLDPIDGTKSFISGVPLFGTL
                     IALLHRGQPIVGAIHQPILRQLVIGDGTTTTLNGRPVRCRRTTRIEDATFLTSDPLNP
                     AQYQPKSYTAYEALARRARLVRTWGDAYGYLLVACGWADVMLDPVMNPWDIAALVPVI
                     RGAGGTITDWSGR"
     gene            782..>1225
                     /locus_tag="VEA63_04290"
     CDS             782..>1225
                     /locus_tag="VEA63_04290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HYD83239.1"
                     /translation="MNWIEQPIFFVDFEGSQTSGVLEYGVAEVRGARIVATHTRLCQA
                     TGMVRDEDVAVHRLSAPALSLWAPFADDWEFFAGLRERGPLAAHYAGVENGLLKAVWP
                     YPRNSPDFARPGERVIEWGPWIDSARLYQQFYPELGSGKLELLVTA"
BASE COUNT          216 a          334 c          452 g          223 t
ORIGIN      
        1 aacggccgga ccagtcggtg atcgtgccgc ccgcgccgcg gatcaccggg acgagcgcgg
       61 cgatgtccca cgggttcatc accggatcga gcatcacgtc ggcccaaccg cacgcgacga
      121 ggagataacc gtaggcgtcg ccccacgtgc gcacgagtcg cgcgcggcgg gcgagggctt
      181 cgtaagcggt gtaggacttt ggctgatact gcgcggggtt gaggggatcg ctggtgagaa
      241 acgtcgcgtc ttcgatgcgc gtggtgcggc ggcaacgaac gggccggccg ttgagcgtgg
      301 tggtggtgcc gtcgccgatc acgagctgac ggagaatcgg ttggtgaatc gcgccgacga
      361 tcggctggcc acgatggagc agcgcgatga gcgtgccgaa gagcggcacg ccggagatga
      421 aggatttcgt gccgtcgatc ggatcgagca cccacacgaa ctcggcgtcg gcgttttcgc
      481 tgccgaattc ttcgccgatg atgccgtgcg tgggaaacgc ttcggcgatg agcgagcgca
      541 gcaacttttc ggcgccgcgg tcggcggcgg tgacgggcga ggcgtcggac ttcacgtcga
      601 cggcgaccgc gtgatcgccg aagaacggac gaatgaagtc gccgcttttt tcggcgagtt
      661 gcgccacgaa actgcgatac aacgctaaat ccacgggaag aagagttgcc ggtgaaacaa
      721 agagagcaca cgacaaaaat cgttcgtgcc tttgaggcgt ttgccgttcc ataccttttc
      781 cgtgaactgg atcgagcagc cgattttttt cgtcgatttc gaaggcagcc agacgtccgg
      841 ggtgctggag tatggcgtcg cagaagtgcg cggcgcgcgg atcgtggcca cgcacacgcg
      901 gctttgccag gcaacgggca tggtgcgcga cgaagacgtg gcggtgcaca gattgagcgc
      961 gccggcgctg tcgttgtggg cgccgtttgc ggacgactgg gaatttttcg cggggttgcg
     1021 cgagcgcggc ccgttggctg cgcattacgc cggcgtggag aacggattgt tgaaagcggt
     1081 gtggccgtat ccgcgcaact cgccggattt cgcgcggccg ggcgagcgcg tgatcgagtg
     1141 ggggccgtgg atcgattcgg cgcggcttta tcagcagttt tatccggagc tcggttctgg
     1201 aaaactggag ctgttggtga cggcg
//
LOCUS       DASPLT010000415         1014 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_127673, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000415 DASPLT010000000
VERSION     DASPLT010000415.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1014)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1014)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1014
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_127673"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..313
                     /locus_tag="VEA63_04295"
     CDS             <1..313
                     /locus_tag="VEA63_04295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD83240.1"
                     /translation="ASASVRVAPGAALTATTPIVRGGGAEQRVAWELLHERTERSEGG
                     DAEERSPAAPLREARESADESEATLAFVAPERRGAYRLFAYVQGAGQTVATANFPFLV
                     E"
     gene            322..>1014
                     /locus_tag="VEA63_04300"
     CDS             322..>1014
                     /locus_tag="VEA63_04300"
                     /inference="COORDINATES: protein motif:HMM:NF012377.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HYD83241.1"
                     /translation="MKTFLLMVLLIGVSALSAAENGFVRTRGTEFVDANGQVLKLRGI
                     NLGNWLLAEGYMFHFQRTASPRQIEDVFAELVGDVTAAEFWAEWRENYIVHDDLRLIR
                     EMGFNSVRVPLNWRLFVSEHAPFRMEGPGWRLLDRVIEWCRAEKLYAVIDLHGAPGGQ
                     TGANIDDSRGRPLLFDDPAAQRLTIELWRAIAERYRDERWVLGYDLLNEPIAHYHDTA
                     RLNPKLGEFYRRL"
BASE COUNT          161 a          245 c          399 g          209 t
ORIGIN      
        1 ggcgtccgcg agcgtgcgcg tggcgccggg tgcggcgttg acggcgacga cgccgatcgt
       61 tcgtggtggc ggtgcggagc agcgcgtcgc gtgggagttg ttgcacgaac gaacggagcg
      121 gagcgaaggg ggcgatgcgg aggagcggtc gccggcggcg ccgctgcggg aagcgcgtga
      181 aagcgcggac gaaagcgaag cgacgctggc atttgtggcg ccggagcggc gtggggcgta
      241 tcgacttttt gcttacgttc agggcgccgg ccagacggtc gcgacagcga actttccttt
      301 cctggtcgag tgatttttcc gatgaaaact tttttgctga tggttctgtt gatcggagtg
      361 agcgcgttga gcgcggcgga gaatgggttt gtgcgcaccc gcggaaccga gtttgtcgat
      421 gcgaacggac aggtgttgaa gttgcgcggg atcaatctcg gcaactggct gcttgccgaa
      481 ggctacatgt ttcattttca gcggacggcc tcgccgcggc agatcgagga tgtgtttgcg
      541 gagctggtcg gcgatgtgac ggcggcggag ttttgggcgg agtggcggga gaattacatc
      601 gtgcacgacg atctgcggct gattcgcgag atgggtttca actcggtgcg cgtgccgttg
      661 aactggcggc tgttcgtgag cgaacacgcg ccgtttcgga tggaaggccc gggatggcgg
      721 ttgctcgatc gcgtgatcga gtggtgtcgt gcggagaaac tttatgcggt gatcgatctg
      781 catggcgcgc cgggcgggca gacgggtgcg aacatcgacg acagtcgggg gcgtccgctg
      841 ttgtttgacg atccggcggc gcagcgtttg acgatcgagt tgtggcgggc gattgcggag
      901 cggtatcgcg acgagcggtg ggtgctcggc tacgatttgt tgaacgagcc gatcgcgcat
      961 tatcacgaca ccgcgcggct gaatccgaag ctcggcgagt tttaccggcg gctc
//
LOCUS       DASPLT010000416         3101 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_87122, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000416 DASPLT010000000
VERSION     DASPLT010000416.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 3101)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3101)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3101
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_87122"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(273..2480)
                     /gene="glgB"
                     /locus_tag="VEA63_04305"
     CDS             complement(273..2480)
                     /gene="glgB"
                     /locus_tag="VEA63_04305"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007363508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HYD83242.1"
                     /translation="MIISTTDLADLLNAQHTSPHSVLGMHPMARGKTKGVVVRALLRN
                     VENCAVVDVAAGEDWPMTSIANDGMFEVFIAKRPEVFGYQLRVKTPAGEMRQFYDPYS
                     FLPTLGPQDLYLFNEGNEHRVYDKLGAHVRTIGGVPGVSFSVWAPAAKQVSLVGNFNH
                     WDGRYHPMRPLGSSGVWELFVPGLEEGELYKYAVRDQRGHVRLKTDPYGTYFEGPPNN
                     ASIVYNTAKFKWSDDAWLEKRRANAGKLDQPMSVYEVHLGSWRRNADDAGRKLDYREA
                     AVQLADYVNEMGFTHVEFMPLAEHPFTGSWGYQVTGFYAPTHRHGSPEDFAWMIDYLH
                     QRGIGVILDWVPAHFPRDSFALAEFDGSHLYEHADPRLGAHMDWGTLIFNYGRNEVRC
                     FLIANALAWIERYHIDALRVDAVASMLYLDYSRKEGQWIPNKYGGRENLEAIDFLKQV
                     NELVHRYHPGALMIAEESTSFPGVTKPPAEGGLGFDFKWNMGWMHDTLRYFAKDPIHR
                     KWHQHDLTFGMLYQYAENFVTVFSHDEVVHGKGSMLFKMGAWHIPEKAAQLRSLYAHM
                     WGYPGKKLLFMGSEFAQSHEWNYDGALDWHLCQYTDHEGVRLLVRDLNKFYATEPVLS
                     QNDLNPQGFRWIACQDAEASVIAYLRTAPDERTLLIVVGHFSGATRDYRIGVPRRGLW
                     REMVNTNSEFYGGTGLGNDGARHTEDSECDGYSQSLSLMLPPHSTFIFKWSAE"
     gene            2699..>3101
                     /locus_tag="VEA63_04310"
     CDS             2699..>3101
                     /locus_tag="VEA63_04310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD83243.1"
                     /translation="MIRRLLAFLALATCAFAANNPEATSIVAGHSETNIRTGETILTD
                     NPRVDYGDVRLTADEITINPSTHIAVARGNATLTRGERRLLSDYIAYNSADGTFEVGD
                     LRVGQYPVYASGSRATGTVDSLTVENARVTVP"
BASE COUNT          604 a         1133 c          799 g          565 t
ORIGIN      
        1 ttcgaactgc acgaccagca agaactgtat taccccgcga tcatgcgcgc catcaaagag
       61 accggcttca aaggcttcgt cgcccaggag ttcgtcccga agcgcgaccc gatcacctcc
      121 ctccgcgaag cggtgaaact ctgcagcgtc tagtccgacg cgcccctacc tcaaaggcag
      181 gaacgccgct ccgaggcgcc cttggttcgg cgagcgcaac ccgctcagcc gctcgcagat
      241 cgagcgggtc cacggcgtgc ctccccgccc cgtcactccg ccgaccactt gaaaataaac
      301 gtcgagtgcg gcggcagcat caaactcagc gactgactgt agccgtcgca ttcagaatcc
      361 tccgtgtgcc gcgcgccatc gttccccagc ccggtgccac cgtagaattc gctgttcgtg
      421 ttcaccatct cgcgccacag cccgcggcgc ggcacgccga tccgatagtc gcgcgtcgcg
      481 ccggagaaat gtccgaccac gatcaataac gtgcgctcgt ccggcgccgt tcgcagatac
      541 gcgatcacac tcgcctcggc atcctgacac gcgatccagc gaaacccttg cgggttcaaa
      601 tcgttttggc tgagcaccgg ctccgtcgca taaaatttat tcagatcgcg caccagcagc
      661 cgcacgcctt cgtgatccgt atactggcaa agatgccagt cgagcgcgcc gtcgtagttc
      721 cattcgtgcg actgcgcgaa ctcgctgccc atgaagagca gtttctttcc cggataaccc
      781 cacatgtgcg cgtaaagcga acgcagttgc gccgctttct ccggaatgtg ccacgcgccc
      841 atcttgaaga gcatcgagcc ttttccgtgc accacttcgt cgtgcgaaaa caccgtcacg
      901 aaattctccg catactgata cagcatgccg aacgtcaggt cgtgctggtg ccacttccgg
      961 tgaatcggat ctttcgcgaa gtagcgcaac gtgtcgtgca tccatcccat gttccacttg
     1021 aagtcgaacc cgagcccgcc ctccgccggc ggcttcgtca ctccgggaaa cgacgtgctc
     1081 tcctccgcga tcatcaacgc gccaggatga taccgatgca cgagttcgtt cacctgcttc
     1141 aggaaatcga tcgcctcgag attctcgcgg ccgccgtatt tgttcgggat ccactgccct
     1201 tccttgcgcg agtaatcgag atacaacatc gacgccaccg catccacgcg caacgcgtcg
     1261 atgtgatagc gctcgatcca cgccagcgca ttcgcgatca ggaaacagcg cacctcgttc
     1321 cgcccatagt tgaagatcaa cgtcccccaa tccatgtgcg cgcccaatcg cggatccgca
     1381 tgctcgtaga gatgcgagcc gtcgaattcc gccagcgcga aactgtcccg cggaaaatgc
     1441 gctggcaccc agtcgagaat cacgccgatg ccgcgctggt gcagataatc gatcatccac
     1501 gcaaaatcct ccggcgaccc gtgacgatgc gtcggcgcat aaaatcccgt cacctgataa
     1561 ccccacgacc ccgtgaacgg atgctccgcc aagggcatga actccacgtg cgtgaagccc
     1621 atctcgttca catagtcggc gagttgcacc gccgcctcgc gataatcgag cttccgcccc
     1681 gcgtcgtcgg cgtttcgccg ccacgagccg agatgcactt cgtaaaccga catcggctgg
     1741 tcgagcttgc ccgcgttcgc gcgccgtttt tccagccacg catcgtcgct ccatttgaac
     1801 ttcgccgtgt tgtagacgat cgacgcgttg ttcggcgggc cttcgaaata cgtgccgtaa
     1861 ggatccgtct tcaaccgcac gtggccgcgc tggtcgcgca ccgcgtattt gtagagttcg
     1921 ccttcctcca accccggcac aaacagctcc cacacgcccg acgaaccgag cggacgcatc
     1981 ggatgataac gcccgtccca gtgattgaaa ttgcccacga gcgaaacctg cttcgccgcc
     2041 ggcgcccaca cgctgaacga cacgcccggc accccgccga ttgtgcgcac atgcgcgccg
     2101 agcttgtcgt aaacccggtg ctcgttgcct tcgttgaaca gataaagatc ctgcggcccc
     2161 agcgtcggta aaaacgaata cgggtcgtag aactgccgca tctcccccgc cggggtcttc
     2221 acgcgtaatt gataaccgaa tacctccggc cgcttcgcga tgaacacctc gaacatgccg
     2281 tcgttcgcga tcgacgtcat cggccagtcc tcgcccgccg ccacgtcgac caccgcgcag
     2341 ttctccacgt tccgcagcaa cgcccgcacg accacgccct tcgttttccc gcgcgccatc
     2401 ggatgcattc cgagcaccga atgcggcgag gtgtgctgcg cgttgagcag atcagcgagg
     2461 tcggtggtcg agattatcac gcgggtaagg agttacaggt tcgccaacgt gtgccaaccc
     2521 cgacagtccg tctagccgga agcgcgatat tcccaccgtc gttttctccg aaactgtggg
     2581 aggggcttta cgccccgaca aatcccgcga tcaaccctga tccccgtaaa acccacatcc
     2641 gccccgccac gccgaagggc ttgcttgcgc gcaacccgcc acctttgctc cgcgcatcgt
     2701 gattcgccgc ctgctcgcgt tcctcgctct cgccacctgc gccttcgccg ccaacaaccc
     2761 ggaggctacc tccatcgtcg ccggccactc cgagacgaac atccgcaccg gcgaaaccat
     2821 cctcaccgac aacccgcgcg tcgattacgg cgacgtccgc ctcaccgccg acgagatcac
     2881 catcaacccg tccacccaca tcgccgtcgc tcgcggcaat gccaccctca cgcgcggcga
     2941 gcgccgcctc ctgtccgact acatcgccta caactccgcc gacggcacgt tcgaggtcgg
     3001 cgacttgcgc gtcggtcagt atcccgttta cgccagcggc tcccgcgcaa ccggcaccgt
     3061 cgactcgctc accgtcgaaa acgcgcgcgt cacggttccc g
//
LOCUS       DASPLT010000417         1443 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_271211, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000417 DASPLT010000000
VERSION     DASPLT010000417.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1443)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1443)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1443
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_271211"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1131)
                     /locus_tag="VEA63_04315"
     CDS             complement(<1..1131)
                     /locus_tag="VEA63_04315"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007359743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HYD83244.1"
                     /translation="MGGGRGTRLHPLTQERSKPAVPLAGKYRLVDIPISNCINSEINR
                     IFILTQFNTASLHRHIQSTYHFDPFGGGFVDILSAEQTEKSVDWYQGTADAVRRNLLH
                     FRAFPHEIVLILSGDQLYRMDFREIIEQHIATGADVTLAAVAFPLSKVEGLGLCRVDD
                     DLSIKEFVEKPKDPAIIAGLALSDTLEAKLKTPSDEKRCLASMGIYVFNRSVLTESLE
                     NTMADFGKEVIPGLLGKKKLYAHIFEGYWEDIGTVRAFFEANLALAQPLPPFNFFLPN
                     APIYTQDRYLPASKLNKCAIDHVVIGDGSILTDSSLKHCVLGIRSYVGEGSVLEDVVM
                     MGADHYETADDLAANVEQGRPHLGLGKRCRVKHAIIDKNARIG"
     gene            1102..>1443
                     /locus_tag="VEA63_04320"
     CDS             1102..>1443
                     /locus_tag="VEA63_04320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD83245.1"
                     /translation="MKASASAAAHDDGKHAFWIGHDESKGGLNLASPLLRSLSFAKQK
                     AEIMCGIVGYVGKQKASAIILEGLKRLEYRGYDSAGICVQHGRSLDVAKKAGRVDNLA
                     KLSVHQKFTGTT"
BASE COUNT          303 a          413 c          432 g          295 t
ORIGIN      
        1 cccgatgcgc gcgttcttgt cgatgatcgc atgcttcacc cgacagcgct tgccgaggcc
       61 gagatgcggc cggccttgtt cgacgttagc cgcgagatcg tcggcggtct cgtagtgatc
      121 cgcgcccatc atcacgacgt cttccagcac actcccctcc cccacataag agcggatgcc
      181 gagcacgcag tgtttgagcg atgaatccgt gagaatcgag ccgtcgccga tcaccacgtg
      241 atcgatcgcg cacttgttga gcttcgacgc cgggagataa cggtcctgcg tgtagatcgg
      301 cgcgttcggc aggaagaaat taaacggtgg cagcggttgc gcgagcgcga ggttcgcttc
      361 gaaaaacgcg cgcaccgttc cgatgtcctc ccaataaccc tcgaaaatgt gcgcgtagag
      421 tttctttttg ccgaggagcc ccgggatgac ttctttgccg aaatccgcca tcgtgttctc
      481 cagcgactcc gtgagcacgg accggttgaa aacatagatg cccatcgagg ccaggcagcg
      541 cttctcgtcc gacggcgttt tcaacttcgc ctcgagcgtg tcgctcaacg ccagtcccgc
      601 gatgatggcc ggatccttcg gtttctcgac gaattccttg atcgaaagat cgtcgtccac
      661 gcggcacagc ccaagccctt cgactttcga aagcggaaac gcgaccgcgg cgagcgtcac
      721 gtcggcgccg gtggcgatgt gctgctcgat gatctcgcga aaatccatcc gatagagctg
      781 gtcgcccgag aggatcagca cgatctcgtg cggaaacgcg cggaagtgca gcaggtttcg
      841 ccgcacggcg tcggccgtgc cttgatacca gtccacgctt ttttccgttt gctccgcgga
      901 gagaatatcg acgaacccgc cgccaaacgg atcaaagtga tacgtcgatt gaatatggcg
      961 gtgaagcgac gcggtgttga actgcgtcag gatgaagatc cggttgatct cggaattgat
     1021 gcaattgctg atcgggatat cgacgaggcg atacttgccc gcgagcggaa cggcgggctt
     1081 cgaacgctct tgcgtgaggg gatgaaggcg agtgcctcgg ccgccgccca tgatgacggc
     1141 aagcacgcgt tttggattgg tcatgatgaa agcaaagggg ggttaaacct tgcttcacct
     1201 ttgttgcggt cgcttagttt cgccaaacaa aaagcggaaa ttatgtgcgg aatcgttggc
     1261 tatgtcggta agcaaaaagc atctgcgatc atcctggagg ggctgaagcg cctcgaatat
     1321 cgcggctacg attccgccgg catctgcgtg cagcacggcc ggtcgctcga tgtagccaaa
     1381 aaagcaggac gcgtggacaa cctcgcgaag ctgtcggtgc atcaaaaatt cacgggcact
     1441 acc
//
LOCUS       DASPLT010000418         1101 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_189771, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000418 DASPLT010000000
VERSION     DASPLT010000418.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1101)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1101)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1101
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_189771"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..638)
                     /locus_tag="VEA63_04325"
     CDS             complement(<1..638)
                     /locus_tag="VEA63_04325"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF013323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HYD83246.1"
                     /translation="MATVSEPHTKTSYTLSELMVAAAAREIRDGEVVFVGMRLPLIAF
                     VVAKRTHAPKAVGLFENGVVRSTPASELIYTMADPPNILGATQCLDMTGVMGLLQAGR
                     VHLGFLGAAEVDRFGNLNSTEVRGPKGMVRLPGSGGACDIASLAQRFVVLLEHAKHRL
                     PERVSYTTSPGFGDGNGWRERVGLPRGGPSAVITTKCVLRFEENGEAYLDSV"
     gene            complement(884..>1101)
                     /locus_tag="VEA63_04330"
     CDS             complement(884..>1101)
                     /locus_tag="VEA63_04330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD83247.1"
                     /translation="RWAGQKFHCKTGEPMSDREYEQHVREALPNEEDKKILLDIIRNE
                     PKWIVAKEGAKDPLLTIGEPRKSAVNL"
BASE COUNT          201 a          366 c          296 g          238 t
ORIGIN      
        1 tgcaccgagt cgagatacgc ctcgccgttc tcctcgaaac gcagcacgca cttcgtggtg
       61 atcaccgccg acggcccgcc ccgaggcaat cccacgcgct cgcgccagcc gttgccgtcg
      121 ccaaatcccg gactcgtcgt gtacgacacg cgctccggca ggcgatgctt tgcgtgctcc
      181 agcagcacga cgaagcgctg cgccagcgaa gcaatgtcgc atgctccgcc cgaacccggc
      241 agccgcacca tgcccttcgg cccgcgcacc tcggtcgagt tcaggttgcc aaagcgatcg
      301 acttcggcag ccccgagaaa cccaagatga acgcgtcccg cctgcaaaag gcccatcacg
      361 ccggtcatgt cgaggcactg cgtagcgcca agaatgttcg gagggtcggc catcgtgtag
      421 atcagttccg acgccggcgt cgaacgcacc actccgttct cgaataaacc aacagccttg
      481 ggcgcgtgcg tgcgcttggc gaccacgaac gcgatcagcg gcagccgcat gccgacgaac
      541 acgacttccc cgtcgcgaat ctcgcgcgcc gccgccgcaa ccatcaactc gctaagggtg
      601 tagctcgtct ttgtatgtgg ttcagaaact gtcgccatag gaataaggtt tctgaggtgt
      661 cgaatgtttc ggaggtgtcg gacgtgtcga gtgtttcgga tatttcgatc aattgctgaa
      721 ccacgtccgc gcccctttgt gtccttagca gtgagaatta cccgctgaac gtatcgccaa
      781 accgccctaa acctctgaaa ccttcgaaac ggattttaca cattcactgc tgattttctc
      841 ggcttcccgc ccggggagcg gcacgcttgc ggatcattcg ttgctacaaa ttgactgctg
      901 attttcttgg ctctcctatc gtcagcagag ggtccttcgc gccttccttg gccacgatcc
      961 acttcggctc gttgcggatg atgtcgagca gtatcttctt gtcctcttcg ttgggcagcg
     1021 cctcgcgcac gtgctgttcg tactcgcggt cgctcatcgg ctcgcccgtc ttgcaatgga
     1081 atttctgtcc tgcccatcgt c
//
LOCUS       DASPLT010000419         1532 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_271250, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000419 DASPLT010000000
VERSION     DASPLT010000419.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1532)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1532)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1532
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_271250"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(139..417)
                     /locus_tag="VEA63_04335"
     CDS             complement(139..417)
                     /locus_tag="VEA63_04335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009508984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HYD83248.1"
                     /translation="MATRLPPPTECAQCGAAIPRHARACPECGADERTGWRETSLYDG
                     LDLPDEAWSDTDAPRPPSLRHVNGIAWYWWCVGVAVLLIFALGALALL"
     gene            complement(435..>1532)
                     /locus_tag="VEA63_04340"
     CDS             complement(435..>1532)
                     /locus_tag="VEA63_04340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007359689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HYD83249.1"
                     /translation="AITLFAVLATIALFVGIVIVLGLSISFFSNVLWPLATAGLLALI
                     LRPVVAWFERRLKLRRLASVIILYGVFTLAVTGLLVAVLPPLINQAINFIMYLPELWD
                     SVYTYVRERYPQWVDLVQRQLDNPTVRQISQAVVAQTQALFGEAMPSLKAATGSLLGI
                     FVFFTNLFIVPVYLFFFLLGRREPTADLPKHLPFLKPETRDDLVFLVREFIAIVEAFF
                     RGQVVIGLIMGALLAVGFTLIGLKFGLAIGLALGILNIVPYLGSIVGLAVALPLAFFQ
                     PEGGMQLIGLVLLVFVIVQTIEGWVLTPKIMGFQTGLHPVAIIVAVFFWGTAFDGVLG
                     MLFAIPLTAFFVTAWRLVRRKYFVRPVTVIT"
BASE COUNT          344 a          407 c          566 g          215 t
ORIGIN      
        1 ccgtgggatg gcgacgtgtg aaccaagcgt atttttgcgg gcggtggtcg atgatccccg
       61 ggctcacgcg ccgggctacg tgaatgcggc gtgcgtttga gcgggctgcg gactgctggc
      121 aggcacggag gcttgccgct acaggagagc cagcgcgccg agagcgaaga tgagcaagac
      181 ggcgacgccg acgcaccacc aataccaggc gatgccgttg acgtggcgca gtgaaggcgg
      241 gcgaggggcg tcggtgtcgg accaggcttc gtcggggaga tcgaggccgt cgtagagcga
      301 ggtttcgcgc cagccggtgc gctcgtcggc gccgcattcg gggcaggcac gggcgtggcg
      361 cgggatcgcg gcgccgcatt gggcgcactc ggtcggcggt ggcagacgcg tggccatgtc
      421 gaaacctatc gcaatcaggt gatgacggtg acggggcgca cgaaatattt tcgccggacg
      481 agtcgccacg cggtgacgaa gaacgccgtc agcggaatcg cgaacagcat gccgagcaca
      541 ccgtcgaacg ccgtgcccca gaaaaacact gcgacgataa tcgcgacggg gtggaggccg
      601 gtttggaagc ccatgatctt cggcgtgagc acccagcctt cgatcgtttg cacgatgacg
      661 aaaaccagca gcacgaggcc gatgagttgc atgccgcctt cgggttggaa aaacgcgaga
      721 ggcagggcga cggcgagacc tacgatcgag ccgagatagg gaacgatgtt caaaattccg
      781 agggcgagac cgatcgcgag gccgaatttc agcccgatca acgtaaagcc gacggcgagc
      841 agcgcgccca tgatgaggcc gatgacgacc tgcccgcgga agaacgcctc gacgatcgcg
      901 atgaattcgc gcacgaggaa aaccaagtcg tcgcgcgttt cgggtttcaa gaagggcaga
      961 tgtttgggga gatcggcggt gggctcgcgc cggccgagca ggaagaagaa cagataaacg
     1021 gggacgatga agagattggt gaagaaaacg aaaatgccga ggaggctccc ggtcgcggcc
     1081 ttgagcgacg gcatggcttc gccgaagaga gcctgcgttt gcgcgacgac ggcttggctg
     1141 atctggcgga cggtgggatt atcgagctgg cgttggacga gatcgaccca ttgcggatag
     1201 cgctcgcgca cgtaagtgta gacgctgtcc cagagctccg gcagatacat gatgaagttg
     1261 atcgcctgat tgatgagcgg cgggagcacg gcgacaagga ggccggtgac ggcgagcgtg
     1321 aacacgccgt agaggatgat gacggaggcg agacggcgaa gcttcagccg acgctcgaac
     1381 caggcgacca ccggccggag aatgagcgcg agcaaaccgg cggtggcgag cggccagagc
     1441 acgttggaga aaaagctgat cgagaggccg agaacgatga cgatgccgac gaacaaggcg
     1501 atcgtcgcga gcacggcgaa caacgtgatg gc
//
LOCUS       DASPLT010000420         1680 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_5307, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000420 DASPLT010000000
VERSION     DASPLT010000420.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1680)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1680)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1680
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5307"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..221)
                     /locus_tag="VEA63_04345"
     CDS             complement(<1..221)
                     /locus_tag="VEA63_04345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 factor domain-containing protein"
                     /protein_id="HYD83250.1"
                     /translation="MPLKSKSLSAPADTETFVPTSVELPDAPPSFLEKPERAVAEQVA
                     HGDRSNLQLYLQEIGKTALLTIDEEISLA"
     gene            complement(398..>1680)
                     /locus_tag="VEA63_04350"
     CDS             complement(398..>1680)
                     /locus_tag="VEA63_04350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="chorismate-binding protein"
                     /protein_id="HYD83251.1"
                     /translation="PRKIFVCGPDTTEIRTPGQPTQVVKTPADPLQLIEAEMSGYRPV
                     TLPHLPRFTGGAVGFVGYEYVTRIERSVPAAAKDELGVPLLYFMLSDSLLIFDRAKQT
                     LRLCVNAHVGADAAAAYDAAVAELHHLYDILKHPREIAPAPLVDLPRITVPPGNFTRE
                     RFEQVVEEGKEFIRAGDIIQFVPSQRFSRDFAKSPLDLYRALRTVNPSPYMFILEAGD
                     FSIVGASPEVHVRLTDGRVEIRPIAGTRKRGATAAEDAGLEQELLADEKERAEHLMLV
                     DLARNDIGRVCRFGSIHVPELMIVERYSHVMHIVSQVEGEIAPGRTAYDLLRATFPAG
                     TVTGAPKIRAMQIIAQYEPTQRGSYAGALGYIGYDGNMDTCIMLRTALLKDGKIHIQA
                     GAGVVADSVPASEYQETISKASALFKAVEMAERF"
BASE COUNT          325 a          488 c          544 g          323 t
ORIGIN      
        1 ttcgcgaggg agatttcctc gtcgatggtg agaagggcag ttttgccgat ttcctgaaga
       61 taaagctgca gattgctgcg gtctccatgc gcgacctgtt cggccaccgc acgctccggc
      121 ttctcgagga aggatggagg cgcgtcgggc agctcgacgg aggtcggcac aaaggtttca
      181 gtgtcggcgg gagcggagag agatttggat ttgaggggca tgatgcgtta tttaagttta
      241 gaagcgattg cctctgtctt tgttcccgat ctgtagccaa agttgttcca cttggactta
      301 cgcattctcc ccctctgagg attcaaaaaa ttccgaacaa gcgggcgcga atgtggccca
      361 ccgaccgcca gcgcgagcgc caacgtggcg caactcgcta aaaccgctca gccatctcga
      421 cagctttgaa cagcgccgaa gctttgctga tggtttcctg atattctgag gccgggacgg
      481 agtcggcgac gacgccggcg ccggcttgaa tgtggatctt gccgtccttc aacaaagccg
      541 tgcgcaacat aatgcaggtg tccatgttac cgtcataacc aatataaccg agcgcgccgg
      601 cataggagcc gcgctgggtc ggttcatatt gcgcgataat ctgcatggcg cggatcttcg
      661 gagcgcctgt gacggttccg gcagggaagg tggcccgcaa caagtcgtag gccgtgcggc
      721 ccggcgcgat ctcgccctcg acctgcgaca cgatgtgcat gacgtgtgaa taacgttcca
      781 cgatcatgag ttcgggcacg tgaatcgaac cgaagcggca aacgcggccg atatcattgc
      841 gcgccaaatc gaccagcatg aggtgctcgg cgcgctcttt ttcgtcggcg agcagctcct
      901 gctcaagccc ggcatcttcc gcggcggtgg cgccgcgttt gcgcgtgccg gcgatcggac
      961 ggatttccac gcgtccatcg gtgagccgca cgtgcacttc aggagaagcg ccgacgatgg
     1021 aaaagtcgcc ggcctcgagg atgaacatgt aaggcgacgg attcacggtt cgcagcgcgc
     1081 gatacagatc gagcggcgat ttcgcgaaat cccgcgagaa acgctgcgac ggcacgaatt
     1141 ggatgatgtc gccggcgcgg atgaactcct tgccctcttc gacgacctgc tcgaaacgct
     1201 cgcgcgtgaa gttccccggc ggcacggtga tgcgcggcag gtcgacgagc ggagccggtg
     1261 cgatttcgcg cgggtgtttg aggatgtcgt aaaggtggtg cagctcggcg acggcggcat
     1321 cgtaggccgc ggcggcgtcg gcgccgacat gcgcgttgac gcacaagcgc agcgtttgct
     1381 tcgcgcggtc gaaaatcagc agcgaatccg acagcatgaa atacagcagc ggcacgccga
     1441 gctcgtcttt cgccgccgcg ggcacggagc gctcgatgcg cgtcacgtat tcatagccga
     1501 caaaacccac ggcaccgccc gtgaaccgcg gcaggtgagg caacgtcacc ggccggtagc
     1561 cgctcatttc cgcttcgatg agctggagcg gatcggcggg tgttttcacg acctgcgtcg
     1621 gctggcccgg ggtccggatt tcggtggtat ccggtccgca cacgaaaatc ttccgcggac
//
LOCUS       DASPLT010000421         1903 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_169666, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000421 DASPLT010000000
VERSION     DASPLT010000421.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1903)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1903)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1903
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_169666"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..631)
                     /locus_tag="VEA63_04355"
     CDS             complement(<1..631)
                     /locus_tag="VEA63_04355"
                     /inference="COORDINATES: protein motif:HMM:NF024258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HYD83252.1"
                     /translation="MAIPERFAVIADVHGNAIALEAVLADVARRGTRMIVNLGDNANG
                     PLEPARCVELLRSRVTVNVRGNGDRGVADAETSLRSAKFARERLDQAAIAWLGELPRV
                     HEGDGWTAFHAAPENDEEYLLEEVSAGGVTLREAAAVAERLRGVRGELLLCGHTHVPR
                     AVRLGDARLVVNPGSVGLPGYSVANPFPHKMENGSPHARYAIVERGAAGW"
     gene            750..1136
                     /locus_tag="VEA63_04360"
     CDS             750..1136
                     /locus_tag="VEA63_04360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD83253.1"
                     /translation="MNSPNASTHNSYDGLSVRSLDVFPLTCERCQRRFTSLKDFVART
                     TPIFRSTGLMERDDPVIGSFVLLLRNCLCGTSLALRCNDRRDHSPDGRARRSQFDELV
                     RLLTDAGVDSEEANSAARRALQIPKE"
     gene            1141..1692
                     /locus_tag="VEA63_04365"
     CDS             1141..1692
                     /locus_tag="VEA63_04365"
                     /EC_number="1.-.-.-"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF013412.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HYD83254.1"
                     /translation="MAKFFPELSPAHRRFIAEQKIFFVASAPADGRVNLSPKGMDTFR
                     VLNAKRIGYLDATGSGNETAAHLADNGRLTVMFCAFAGAPLILRVYGRGRSVQPRDAD
                     WTQLRPLFGPPIPGERQLIVADVESVQTSCGFAVPLMNYEADRTILNDWAEKKGPDGV
                     AAYWAEKNQRSIDGLPTGLLSEP"
BASE COUNT          324 a          762 c          478 g          339 t
ORIGIN      
        1 tccatccggc tgcgccgcgc tccacaatcg cgtagcgcgc atgcggactc ccgttctcca
       61 ttttatgcgg gaacggattc gcaacgctgt aacccggcag gccaacactc cccggattca
      121 ccaccaaccg cgcatcgcct aaacgcaccg cgcgcggcac gtgcgtgtgt ccgcacaaca
      181 gcagttcacc ccgaacgccg cgcaaccgct ccgccaccgc cgcagcctcg cgcaacgtca
      241 cgccacccgc gctcacctcc tccaacaaat actcctcgtc attctccggc gccgcgtgaa
      301 acgccgtcca gccgtcgcct tcatgcacgc gcggcaactc gcccagccac gcgatcgccg
      361 cttgatccaa ccgctcccgc gcgaacttcg ccgaacgcaa actcgtctcc gcatctgcga
      421 cgccgcgatc gccgtttccg cgaacgttca ccgtcacccg cgatcgcagc agctccacgc
      481 acctcgccgg ctcgagcggc ccattcgcgt tgtcgccaag gttcacgatc atcctcgtcc
      541 cacgccgcgc gacatccgcc aacaccgcct ccagcgcgat tgcattgcca tgcacgtctg
      601 caatcaccgc aaaacgctcc ggaatcgcca tgatcttcaa caacacccgt tgccatttca
      661 ggcaagccct tcggctgacg aggagctcct ccggccgttt cgctcttgcc aatgaggagc
      721 gacattcctg cgctgtctcc gcacccgtca tgaattcgcc gaacgcctcc acccacaatt
      781 cttacgatgg gctcagcgtt cggtcgctcg acgtgtttcc tctcacgtgt gagcgctgcc
      841 agcgtcgctt caccagcctc aaggacttcg tcgcacgcac cacgccgatc tttcgatcga
      901 ccggtttgat ggagcgcgac gaccccgtca tcggctcgtt cgtgctgctg ctccgcaact
      961 gcctctgcgg aacctcgctc gcgctccgct gcaacgatcg tcgcgatcac tcgcccgatg
     1021 gtcgagctcg tcgcagccag ttcgacgagc tcgtccgctt gctgacggac gccggcgtcg
     1081 attccgagga agccaactct gccgcgcgcc gcgcgcttca aattccgaaa gagtgagccg
     1141 atggcgaagt tctttccgga actctcgccg gcgcaccgcc ggttcatcgc cgagcagaaa
     1201 attttcttcg tcgccagcgc gcccgccgat ggccgcgtga atctctcgcc gaaaggcatg
     1261 gacacgtttc gcgtcctcaa cgccaaacgc atcggttatc tcgacgccac cggcagcggc
     1321 aacgaaaccg ccgcgcacct cgccgacaac ggccgcctca ccgttatgtt ctgcgcgttc
     1381 gccggcgcgc cgctcatcct ccgcgtctac ggccgcggcc gctccgtgca accgcgcgac
     1441 gccgattgga cgcaactccg cccgctcttc ggcccgccga ttcccggcga acgccagctc
     1501 atcgtcgccg acgttgaaag cgtgcagacc tcctgcggct tcgccgtccc gctcatgaac
     1561 tacgaagccg atcgcacgat tctcaatgat tgggccgaga aaaaaggccc cgacggcgtc
     1621 gccgcttatt gggctgaaaa aaatcaacgc agcatcgacg gcctcccgac cggcctcctc
     1681 tccgaaccgt aacgccggcc tcagggccgc acgttcgcgc gtcgcacccc tcgcgcagtc
     1741 gctttcgcgc aacccaaccg cgcggtcacc gccgccccgt cgtcatccgc tccgcaattt
     1801 gcctccgttt gcacccgcgc ccaccgcact cacgctcgcc acgccgttca tcgccgctat
     1861 ctccttgccg atgatccgcg ccctcgtctt cgattttgac ggc
//
LOCUS       DASPLT010000422         1159 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_169679, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000422 DASPLT010000000
VERSION     DASPLT010000422.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1159)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1159)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1159
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_169679"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..784)
                     /gene="cysK"
                     /locus_tag="VEA63_04370"
     CDS             complement(<1..784)
                     /gene="cysK"
                     /locus_tag="VEA63_04370"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009509016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HYD83255.1"
                     /translation="MAKIHNDITETIGNTPLVRLNRTAAAHGAQAEILLKLEFFNPLS
                     SVKDRIGFAMIDDALKSGKINAKSVLIEPTSGNTGIALAFVAAAKGLKLILTMPETMS
                     VERRKLLKILGARLVLTEGAKGMKGAIAKAEELAAKIPNSVILQQFANPANPAIHRKT
                     TAEEIWNDTDGKVDIIVSGIGTGGTITGVGEVLKPRKPSLKIVAVEPDASPILSGGTP
                     GPHKLQGLGAGFIPAVLNTKVYDEVIRVKEVDAGPVSKQVNQL"
     gene            complement(878..>1159)
                     /locus_tag="VEA63_04375"
     CDS             complement(878..>1159)
                     /locus_tag="VEA63_04375"
                     /inference="COORDINATES: protein motif:HMM:NF024265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TOBE-like domain-containing protein"
                     /protein_id="HYD83256.1"
                     /translation="EFLGNVNVLRAQSLVQAGIAPSGAAVAENGQVYVRPHDIEVSPD
                     EPGAVGIVAVLRYIHAAGAQARLTLEQVHNREPVDVEISRTELESLGLR"
BASE COUNT          204 a          383 c          332 g          240 t
ORIGIN      
        1 cgagttggtt cacctgcttc gagaccggac cggcgtcgac ttccttcacg cgaatcactt
       61 catcgtaaac tttcgtgttc aacaccgccg ggatgaaacc cgcgccgagc ccctgcaact
      121 tgtgcggacc aggcgtgcca ccggaaagaa tcggcgacgc atccggctcc accgccacaa
      181 tcttcagcga cggtttgcgt ggtttcagca cttcgccgac gcccgtgatc gtgccgcccg
      241 tgccgatgcc cgacacgatg atgtccacct tgccgtccgt gtcgttccag atttcttccg
      301 ccgtcgtctt gcgatgaatc gccggattag ccgggttcgc gaattgctga agaatcacgc
      361 tattcggaat cttcgccgcg agctcttccg ctttcgcaat tgcgcccttc atgcccttcg
      421 cgccctcggt gagaaccaga cgcgcgccga gaattttcaa cagcttgcga cgttcgacgc
      481 tcatcgtctc cggcatcgtg aggatcagct tcaggccttt cgccgccgca acgaacgcca
      541 acgcgatgcc ggtgttaccg gaggtcggtt cgatcaacac cgacttcgcg ttgatcttcc
      601 ccgacttcaa cgcgtcgtcg atcatcgcga aaccgatgcg gtccttcacg ctcgagagcg
      661 ggttgaagaa ctcgagcttc agcaaaatct ccgcctgcgc gccgtgcgcc gccgcggtgc
      721 gattgagccg caccaacggc gtgttgccga tcgtttcagt gatgtcgtta tgaattttgg
      781 ccatggttgg gatgtggttg agattggggt ggcggttaaa tcgcgtagtc gtcgctgaac
      841 gaatgcgact gacggagccg aaggcgcacg aggtcgttca cctcagcccg aggctttcga
      901 gctcggtgcg agaaatctcg acgtcgacgg gctcgcgatt gtgcacctgc tcgagcgtga
      961 gccgcgcctg cgcaccggcc gcatgaatat accgcagcac cgcgacgatg cccaccgcgc
     1021 ccggttcatc cggcgagacc tcaatgtcgt gcggccgcac gtaaacctgt ccgttctccg
     1081 cgaccgccgc gccgctcggc gcgatgccgg cctgcacgag cgactgcgcg cgcagcacat
     1141 tcacgttgcc cagaaattc
//
LOCUS       DASPLT010000423         2823 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_292373, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000423 DASPLT010000000
VERSION     DASPLT010000423.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 2823)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2823)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2823
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_292373"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..350)
                     /locus_tag="VEA63_04380"
     CDS             complement(<1..350)
                     /locus_tag="VEA63_04380"
                     /inference="COORDINATES: protein motif:HMM:NF016430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor"
                     /protein_id="HYD83257.1"
                     /translation="MAPFDADALANHRELVSRMASGDAEALQLLYLQFSGQINGIVRR
                     ILEDPEDAREAVQDTFIKAWRQAATYRRDRGEVVSWLVFIARHSAIDRLRKGARRRLL
                     HEALQREPSEIAMPA"
     gene            complement(386..886)
                     /locus_tag="VEA63_04385"
     CDS             complement(386..886)
                     /locus_tag="VEA63_04385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD83258.1"
                     /translation="MNIMETLLLRFMRVVLTAAGLSLPAIGHAQFYIEAGVSAAGVGS
                     AQFERRSAPFSGDEIEGAGLLFASVPSLGKQLGLRASYHYVGDTKVTAGRGHQPDVLQ
                     QASGAVVYGLAPELRWAAADQAEMAFTSGLSGERRSESFFAHPYAVFPEAPKWDFNRV
                     ATDRNH"
     gene            complement(1004..2050)
                     /locus_tag="VEA63_04390"
     CDS             complement(1004..2050)
                     /locus_tag="VEA63_04390"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006222 - UMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HYD83259.1"
                     /translation="MGGFYEKVLRRGLFKLDAEHAHELAVDAMALLGKLAPACRVLER
                     MQALPTSARPIEAFGLKFPNAVGLAAGFDKNARAWPAAAALGFGHVEVGTVTALPQPG
                     NDRPRVFRYPAEKAVINRMGFNNAGSEAVAAALSRLPGRAQRRIPLGINLGKSKVTEI
                     GKATADYLASFARLADHADYVAINVSSPNTPGLRQLQDEDRLRELLGALATANRERKQ
                     RVPLLLKIAPDLTFPQVDAVLAAIFEFGFDGIIATNTTLARPGFFTGVNEAGGLSGRP
                     LRRRSTEIVSYIARATGYRLPIIGVGGIEDVESAAEKLDAGATLVQIYTGMIYRGPSL
                     AAEVAHGLIDRQRR"
     gene            2234..>2823
                     /locus_tag="VEA63_04395"
     CDS             2234..>2823
                     /locus_tag="VEA63_04395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD83260.1"
                     /translation="MAKATSTLDWCIVRLGEDHNWWVNETSDPVRWDVDGLSIIDPRQ
                     LSHLVDLVDPLRDYGFDQDNMERAFILFRIEKDLGNGKVRLKRTKESLFESDEKLFAL
                     PDILDEENGPYADLLDHLTRCRVKMLNDLFEFESKLTVDEVEDELREDQNSHFIEGKA
                     VHSFEELTSILDFMPAGYESDDETPAKNLDDEGDAD"
BASE COUNT          580 a          995 c          747 g          501 t
ORIGIN      
        1 gcgggcatcg cgatctccga cggctcgcgc tgcaacgcct cgtgcagcag ccggcgccgc
       61 gcgcctttgc gcaatcgatc gatcgccgaa tgccgcgcga tgaacaccag ccacgaaacc
      121 acttcgccgc gatcgcgccg gtaagtggcc gcctggcgcc acgccttgat gaacgtgtct
      181 tgcaccgctt cgcgcgcgtc ctccggatcc tcgagaatac ggcggacgat gccgttgatc
      241 tgtccgctga actgcagata cagcaactgc agcgcctcgg cgtcgcccga cgccatgcgc
      301 gagacaagtt cgcgatgatt cgcaagagca tcggcatcga agggcgccac agtgcgcgat
      361 ggcctactcg cctggaccac ggagatcaat gattcctatc ggttgcgacg cggttaaaat
      421 cccatttcgg agcctcggga aaaaccgcgt aggggtgagc gaaaaacgac tccgaccgcc
      481 tttccccgct caatcccgac gtgaacgcca tctcagcctg atccgccgct gcccagcgca
      541 actccggcgc gagtccgtaa acgactgccc ccgacgcctg ctgtaacacg tccggctgat
      601 ggccgcggcc cgcggtgacc ttggtgtctc caacataatg atacgatgcg cgtaaaccga
      661 gttgtttgcc cagcgaaggg acgctcgcga agagcaaccc tgctccctca atttcgtcac
      721 cggagaacgg tgccgaccgg cgttcaaact gcgcgcttcc caccccggcg gcgcttacgc
      781 ctgcttcgat gtaaaactgc gcgtggccaa tcgccggcag cgacagccct gcggccgtca
      841 gcacgacgcg catgaatcga agaagaagcg tttccataat gttcatggtg gtgaacacgt
      901 ctgattacgc ccttcgcctg ccggctgagt cacccgcgcc aaaaaaatgc gcgcacatct
      961 cacggcgccg cgcatcgccg gcccgcgccc acgcgccggt ccgctaacgc cgctgccgat
     1021 cgatcaagcc gtgcgccacc tccgccgcga gcgatggtcc ccgataaatc atcccggtat
     1081 aaatctgcac cagcgtcgcg cccgcatcga gtttctcggc ggcactttcc acgtcctcga
     1141 ttccacccac tccgatgatc ggcaaccgat aaccggtcgc ccgcgcgatg tagctcacaa
     1201 tctccgtcga ccgccggcgc aaaggccggc cgctcagtcc gcccgcttcg ttgacgcccg
     1261 tgaaaaatcc cggacgcgcc agcgtcgtgt tcgtcgcgat gatcccgtcg aagccgaatt
     1321 cgaaaattgc cgcgaggacc gcgtcgactt gtggaaacgt caggtcgggc gcgatcttca
     1381 gcagcaacgg cacgcgttgc ttccgttcgc gattcgccgt cgcgagcgca ccgagcaatt
     1441 cgcgcaaccg gtcttcgtcc tgaagctggc gcaatcccgg cgtattcgga ctgctgacat
     1501 tgatcgcaac gtaatcggcg tgatccgcca accgcgcgaa actcgccaga tagtccgccg
     1561 tggccttgcc gatttcggtc accttggatt tcccgagatt aatcccgagc ggaatccgcc
     1621 gctgtgcgcg tcccggcaaa cgcgaaagcg ccgccgcgac cgcctcggaa cccgcattgt
     1681 tgaaacccat gcgattgatg accgcctttt ccgccggata acgaaacaca cgcggccgat
     1741 cgttccccgg ctgcggaagc gccgtgacgg ttcctacttc cacgtgacca aatcccagcg
     1801 ccgccgccgc cggccaggcc cgcgcattct tgtcgaagcc ggccgccaaa cccacggcat
     1861 tcggaaactt caagccaaac gcctcgatcg gccgcgccga cgtcggcagc gcctgcatcc
     1921 gctcgagcac gcgacacgcc ggcgcgagtt ttcccagcag tgccatcgca tccaccgcca
     1981 attcgtgcgc atgctccgca tccagcttga acaagccgcg gcgcaagacc ttctcgtaga
     2041 aaccgcccat gacgcgcgac actgaaaacg caccttcaaa atgcaaggcg gagaaatccc
     2101 taaaaccctc tggacggcgc ggatttccgc aggaaaacgt attctgattt ttgatgcaaa
     2161 aacagcgcgc ttttgtgctt gttttttcaa cctcccctcg cacgctgcgc caaaattcat
     2221 ccctttccct ttcatggcca aagcaacttc aaccctcgac tggtgtatcg tccgactcgg
     2281 cgaggatcat aactggtggg tcaacgaaac gagcgatccc gttcgctggg acgtcgatgg
     2341 cctcagcatc atcgaccccc gccagctctc ccacctggtc gatctcgtcg atccccttcg
     2401 cgactacggt tttgatcagg ataacatgga gcgcgcgttc attctcttcc gcatcgagaa
     2461 ggacctcggc aacggcaagg tccgcctcaa gcgcacgaag gaatcgctct tcgaatccga
     2521 cgaaaaactc ttcgcccttc ccgacatcct cgacgaggaa aacggcccct acgccgacct
     2581 cctcgatcac ctcacgcgtt gccgcgtgaa gatgctgaac gatctcttcg aattcgaatc
     2641 caagctcacc gtcgacgaag tcgaagacga gctccgcgag gaccagaaca gccacttcat
     2701 cgaaggcaaa gccgttcact ctttcgaaga actcacctcc atcctcgact tcatgcccgc
     2761 cggctacgag tccgatgacg agacgcccgc caagaatctc gacgacgaag gcgacgccga
     2821 cga
//
LOCUS       DASPLT010000424         1470 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_44827, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000424 DASPLT010000000
VERSION     DASPLT010000424.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1470)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1470)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1470
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_44827"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..271
                     /gene="pgi"
                     /locus_tag="VEA63_04400"
     CDS             <1..271
                     /gene="pgi"
                     /locus_tag="VEA63_04400"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375827.1"
                     /note="functions in sugar metabolism in glycolysis and the
                     Embden-Meyerhof pathways (EMP) and in gluconeogenesis;
                     catalyzes reversible isomerization of glucose-6-phosphate
                     to fructose-6-phosphate; member of PGI family; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HYD83261.1"
                     /translation="LVGINAYHQPGVEAGKKAAGSVIALKKRLVAQLQSTLGAFATAE
                     EIAEKLGADTSTELVFKILEHLAANRGSGVKKRARAPWYQSRYGM"
     gene            528..689
                     /locus_tag="VEA63_04405"
     CDS             528..689
                     /locus_tag="VEA63_04405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009511453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD83262.1"
                     /translation="MSPKKSLLGFLLVVFIALTAGCMSAPKDTSIPWSRPADWEGQIP
                     GMSGSGMGR"
     gene            783..>1470
                     /gene="rsmI"
                     /locus_tag="VEA63_04410"
     CDS             783..>1470
                     /gene="rsmI"
                     /locus_tag="VEA63_04410"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007360693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HYD83263.1"
                     /translation="MDNASLTPQPGHLYVVATPIGNLADLTDRARAILGSVDVIACED
                     TRTTGALLTRLGFHKTLVAYHEHNEIEAAERLADQLAAGKSIAVVSDAGTPALSDPGF
                     RIVRACRRRSLPVVPVPGPSALTAVLSASGLPTNGFLYVGFLPPKSAARIAFLEKYRT
                     FDYTLALYESCHRIDKAVDEIVATLGGERVICVAKEVTKLHETFLVGPASDVQSRLAK
                     TSLKGEFVLLI"
BASE COUNT          281 a          561 c          368 g          260 t
ORIGIN      
        1 gctcgtcggc atcaacgcct accatcaacc cggcgtcgaa gccggcaaga aagccgccgg
       61 cagcgtcatc gcgctgaaaa aacgcctcgt cgcgcagctc cagtcgacgc ttggcgcttt
      121 cgccaccgcc gaggaaattg cggaaaaact cggcgccgac acgtcgaccg aactcgtatt
      181 caagattcta gaacacctcg ccgccaaccg cggcagcggc gtcaaaaaac gcgcccgcgc
      241 cccttggtat caatcccgct acggcatgta gcctgggagt ttcggcgcat cgacagtagg
      301 gcagtcgtcc caactgcccc gagttcccac ctccaccctg ccttccaccc gcgaattacg
      361 cgaatcaccg cgaagattac ggacaatctg tctctcagtt ctatcctcag ccaattcgag
      421 gcaattcgcg tgattcgcgg gcaactcttc cggcttgcca ctttttaccc ctccgcgacc
      481 ttgcagccgg ccgtttcgag cgtaataacc actcaaccga tctcataatg tctccgaaaa
      541 aatcccttct cggcttcctc ctcgtggtct tcatcgctct caccgccggc tgcatgtcgg
      601 cgccgaaaga cacctccatt ccgtggagcc gtcccgccga ctgggagggc cagatccccg
      661 gcatgagcgg cagcggcatg ggccgctaaa ccgaagagtt atcgcacggc cccgacacgt
      721 cggggccttt tttatttcgc ctgtagccct gcgcgtgagc gcggggactt cacccaggtc
      781 acatggacaa cgcctccctc acgccgcagc ccggccatct ctacgtcgtc gccacgccga
      841 tcggcaatct cgccgatctc accgaccgcg cccgcgccat cctcggcagc gtcgacgtca
      901 tcgcctgcga ggacacgagg accaccggcg cactcctcac ccgcctcggc ttccacaaaa
      961 ccctcgtcgc ctatcacgag cacaacgaaa tcgaagccgc cgaacgcctt gccgatcaac
     1021 tcgccgccgg taaatccatc gccgtcgtga gcgacgccgg cacgcccgcg ttgagcgacc
     1081 ccggcttccg catcgtccgc gcctgccgcc gccgcagcct ccccgtcgtt cccgtccccg
     1141 gccccagcgc gctcaccgcc gtcctcagcg cgagcggcct gccgacgaac ggtttcctct
     1201 acgtcggttt tctcccgccc aaatccgctg cgcgcatcgc gtttctggaa aagtatcgaa
     1261 ctttcgacta cacgctcgcg ctctacgaaa gctgccaccg catcgacaag gcagtcgatg
     1321 aaatcgtcgc cacgctcggc ggcgaacgcg tgatctgcgt cgcgaaggaa gtcacgaaac
     1381 tccacgaaac ctttctcgtc ggccccgcga gcgacgtgca atcccgcctc gccaaaacca
     1441 gtctcaaggg cgaattcgtt ctcctcatcg
//
LOCUS       DASPLT010000425         3083 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_87197, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000425 DASPLT010000000
VERSION     DASPLT010000425.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 3083)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3083)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3083
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_87197"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(173..298)
                     /locus_tag="VEA63_04415"
     CDS             complement(173..298)
                     /locus_tag="VEA63_04415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD83264.1"
                     /translation="MKAPRLNRLFNPRTRRCFDVAIDHGFFNEASFLVASKICHG"
     gene            complement(486..1583)
                     /locus_tag="VEA63_04420"
     CDS             complement(486..1583)
                     /locus_tag="VEA63_04420"
                     /inference="COORDINATES: protein motif:HMM:NF025957.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HYD83265.1"
                     /translation="MLVVGTVGAQTAPAPAAAPSAAPDAEPALTWHDVHGWGVEGRAW
                     EDLDRLRWFDRLPAVAKGKVTENVWNLSRDSAGMLVHFRTDATTIWADYTLRKEKLAG
                     ANMTAIGASGLDLYARDDSGRWRWAGVTRPAATQVRQQIVSDLAPGLREYALYLPLFN
                     GIEQLAIGVPPDAEFTGIAPRSEKPIVFYGTSITHGASASRPGMTHPAILGRRLNLPV
                     INLGFSGNGRMDAAVGELLVKIDAAVYVIDCLPNMGAALVRERCLPLVRQLRAARPDT
                     PIVLVEDRRNANAWILPSREQHHTDNHAALRECFDALQAEGVSGLHYLPGDDLIGSDS
                     EGTTDGSHPNDLGFVRQADVFEPVLRAALGR"
     gene            complement(1794..>3083)
                     /locus_tag="VEA63_04425"
     CDS             complement(1794..>3083)
                     /locus_tag="VEA63_04425"
                     /inference="COORDINATES: protein motif:HMM:NF013239.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 31 protein"
                     /protein_id="HYD83266.1"
                     /translation="DVYREAARRFFPSGGRLPAPELFTRPQYNTWIELLYDQREERIL
                     AYARSLVEQGYPPGVLMIDDNWEEDYGEWEFSARRIPHPKRLVRELKEMGFKVMLWVC
                     PFVSPDGEMYRELEKRGFLLREAMVDANRQRVDARSCPAIVRWWNGASAVLDLSHPEA
                     RAWFKAQLDRLVADYGIDGFKFDAGDSGSYVPPPGRIFLSHEPRTPEEHTMDYVRLGV
                     EYPFNEYRAGWKLAGRPLAQRLRDKPHSWEALQDLIPGIVAQGLMGYAFTCPDMIGGG
                     HAGSFEEVSTVDAELVVRAAQVHALMPMMQFSVAPWRVLSPEQNQICVAAAKLHARFG
                     DRILALAREAAATGEPIVRPLAWEWPRGGYEEVRDQFLLGQDIMVAPVLEKGARARTV
                     VFPPGRWLGDDGVVVDGPAQKEISVPLERLPYYQLQR"
BASE COUNT          612 a         1082 c          879 g          510 t
ORIGIN      
        1 tgctcgatca tccgggaaaa cagcgcgcgc ggcagttcgc ggccatacac gttggcgacg
       61 tcggtgcgaa gcaccagcgc cggtttgccg cgcacgggca gcgcctgtaa atgcggcgcc
      121 tggccgacgg tgagttgaat cgcatcgggc gctgcagcga ccagcgtctc gatcacccgt
      181 ggcagatttt cgatgccacg agaaacgacg cttcgttgaa aaaaccgtgg tcgatcgcga
      241 catcgaaaca gcgccgcgtg cgcggattga aaaggcgatt cagtcgaggg gctttcatga
      301 acgcagccat taaaccaaca acgcgcgccg ctcgctacct cttaatgcgc aatcgctgac
      361 actattatct ccgcgcgctc ctgccgtaaa aggtggagcc cgacgtcccg tcgggctggc
      421 agcgttcgct cgcgaaacaa cccgacgagg acgtcgggtc cccctgcaac tgcaaggatg
      481 cggcttcagc gtccaagcgc ggcgcgtaac accggctcga atacatccgc ctgacgcaca
      541 aacccgaggt cgttcggatg cgaaccatcc gtggtgcctt cgctgtcgct gccgatcagg
      601 tcgtcacccg gaaggtagtg caagccgctc acaccctccg cctgcaaagc atcgaaacac
      661 tcgcgcaacg cggcgtggtt gtccgtgtga tgctgctcgc gcgacggcaa aatccaggcg
      721 ttcgcattcc gccggtcctc gaccagcacg atcggcgtat ccggccgcgc cgcccggagt
      781 tgacgcacca gcggcaagca ccgttccctc acgagggccg cgcccatgtt cggcagacaa
      841 tcgatcacat agaccgccgc atcgattttc acgagcagtt cgcccaccgc ggcgtccatc
      901 cggccgttgc ccgaaaagcc gagattgatc acgggcaaat tcagccgccg acccagaatc
      961 gccggatgcg tcatgcccgg acgagacgcg ctcgccccat gcgtgatcga cgttccataa
     1021 aacacgatcg gcttttcgct gcgcggtgcg atgccggtga actccgcgtc tggaggaacg
     1081 ccgatcgcga gctgctcgat gccgttgaag agcggcagat acaacgcata ctcgcgcagc
     1141 cccggcgcga ggtccgagac gatctgctgg cgaacctgcg tcgcggccgg acgcgtcacg
     1201 ccggcccagc gccagcgacc ggaatcgtcg cgcgcataaa gatccaatcc gctcgcgccg
     1261 atcgccgtca tattcgcccc ggccagtttt tctttccgca aggtgtagtc cgcccaaatc
     1321 gtcgtcgcat cggtgcggaa gtggacgagc atccccgccg agtcgcggct caagttccag
     1381 acgttctccg taaccttgcc cttcgccacc gccggcaagc ggtcaaacca gcgcaaccgg
     1441 tcgagatctt cccatgcgcg gccctcgaca ccccaaccgt gaacgtcgtg ccatgtcagc
     1501 gcaggttccg catcgggggc ggccgacggc gccgccgcgg gagcaggagc ggtctgcgca
     1561 ccgacggtgc caaccaccag cataagaaaa gggaaaacga atcggaggag aacgctcatc
     1621 atgcctcagc aaaggacgtt tgccctcgac aagccaagct ggaacaaacg gagcgcggcg
     1681 cgctggcgcc gtctcgcgat ggctgccgat cgcgactgcg cacgacgcgt gaattttttt
     1741 gagctcacgt cgacgcgagt taacccgcgc tcctcccgca tgagcccgtc tggctaccgt
     1801 tggagttgat agtaaggcaa gcgctccaac ggaacggaga tttccttctg cgcaggccca
     1861 tccaccacca ccccatcgtc gccgagccac cgtccgggcg gaaaaaccac ggtgcgcgct
     1921 cgcgctccct tctccagcac cggtgccacc atgatatctt ggccgagcag gaactggtct
     1981 ctcacctctt cgtaaccacc ccgcggccat tcccacgcga gcggacgcac gatcggttca
     2041 cccgtcgccg ccgcttcgcg cgccagtgcc aggatgcgat cgccgaagcg cgcgtgaagt
     2101 ttcgccgccg ccacgcagat ttgattctgc tcgggagaaa gcacccgcca cggcgcgacg
     2161 gaaaactgca tcatcggcat cagcgcgtgc acctgcgccg cgcgcaccac caattcggca
     2221 tcgaccgtcg acacctcttc gaagctgccc gcatgaccgc cgccgatcat gtccgggcag
     2281 gtgaacgcgt agcccatcaa gccctgcgca acgatgccgg gaatcaaatc ctgcagcgcc
     2341 tcccacgagt gaggtttgtc gcgcaaccgc tgcgccagcg gccgacccgc cagcttccag
     2401 ccggcgcggt attcgttaaa tggatattcc acgcccagcc gcacgtaatc catcgtgtgc
     2461 tcctccggcg tgcgcggttc atgcgaaagg aaaatccgtc ccggcggcgg cacgtaagat
     2521 ccgctgtcac ctgcgtcgaa cttgaaacca tcgatgccgt agtcggccac caagcgatcg
     2581 agctgcgcct tgaaccacgc tcgcgcctcg ggatggctca gatcgaggac cgcgctcgca
     2641 ccgttccacc agcgaacgat cgccggacag ctccgcgcat cgacgcgttg cctgttcgca
     2701 tcgaccatcg cctcgcgcag cagaaatcca cgcttctcca gctcgcgata catctcgccg
     2761 tccgggctca cgaagggaca cacccagagc atcactttga aacccatttc cttcagttcg
     2821 cgaaccagcc gcttcggatg aggaattcgt cgcgcggaaa actcccactc gccgtaatcc
     2881 tcctcccagt tgtcgtcgat catcagaacg cccggcggat aaccctgctc cacgagcgaa
     2941 cgcgcgtagg cgagaatcct ttcctcccgc tgatcgtaga gcagctcgat ccaggtgttg
     3001 tattgcggcc gcgtgaacag ctctggcgcc ggcagccgtc caccgctcgg aaaaaaacgc
     3061 cgcgccgctt cacgataaac gtc
//
LOCUS       DASPLT010000426         1129 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_106327, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000426 DASPLT010000000
VERSION     DASPLT010000426.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1129)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1129)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1129
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_106327"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..229
                     /locus_tag="VEA63_04430"
     CDS             <1..229
                     /locus_tag="VEA63_04430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD83267.1"
                     /translation="GQAAAGRDSGAEGEDETTRAIAGAKEVPALEGFVAGHAEFDRRG
                     GRAFAGSLAERAGGARAGQNFEWREAAGKDA"
     gene            299..1039
                     /locus_tag="VEA63_04435"
     CDS             299..1039
                     /locus_tag="VEA63_04435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF899 domain-containing protein"
                     /protein_id="HYD83268.1"
                     /translation="MTTPLFPLPPIVSRAEWLAARKQLLTEEKALTRMRDALAAKRRR
                     LPMVKIEKDYVFAGPAGPVSLREVFGERRQLYVHHFMWNDERGHCPGCSGAADSVFDN
                     PKFRSALEERDVRFVAISRAPLTKIEATRKEKGWTFPWYSSEGDDFNYDFHVTLDETK
                     APIDYNYRNKEELIAAGLKPEDLKGDWTVNSVFLRDGDAVYHTYSAFARGLDHLATVY
                     NFLDLTPYGRQEDWEDSPKGWPQRPTYG"
BASE COUNT          236 a          287 c          416 g          190 t
ORIGIN      
        1 cgggcaggcc gcagccggca gagactccgg cgccgagggc gaggacgaga ccacgagagc
       61 gatagcgggt gcgaaggagg ttccagcgct cgagggattt gtcgcggggc atgcggagtt
      121 cgaccggcgt ggcgggcggg cgttcgcggg cagtttagcg gaacgggccg gcggcgctcg
      181 agctgggcaa aacttcgagt ggcgcgaagc ggcggggaag gacgcttaaa atggaaaagc
      241 gcgagatgtg tccgacggcg gcgcgtttgt tcgtcgagat gttgaaaaag gccaagccat
      301 gacaactccc ttgtttccgt tacccccgat tgtttcccga gccgagtggc ttgctgcgcg
      361 caagcagctg ttaaccgagg agaaagcgct cacgcgaatg cgcgatgcgc tggcggcgaa
      421 gcggcggcga ctgccgatgg tgaagatcga gaaggactat gttttcgcgg ggccggcagg
      481 gccggtgagc ttgcgcgaag tgttcggtga gcggcggcag ctctacgtgc atcacttcat
      541 gtggaacgat gagcgcgggc attgccccgg ttgttccggc gcggcggaca gcgtgttcga
      601 caacccgaag tttcgttcgg cgctcgagga gcgcgacgtg agattcgtgg cgatctcgcg
      661 ggcgccgctg actaaaatcg aagcgacacg aaaggagaaa ggctggacgt ttccgtggta
      721 ttcgtcggaa ggcgacgatt tcaactacga cttccacgtg accctcgatg aaacgaaggc
      781 gccgatcgac tacaactatc ggaacaagga ggagctgatc gctgcggggt tgaagccgga
      841 ggatctgaaa ggggactgga cggtgaacag cgtttttctg cgcgacggcg acgcggtgta
      901 tcacacgtat tcggcgttcg cgcgcggact ggatcacctc gcgacggtct acaactttct
      961 cgatctcacg ccgtatgggc gacaagagga ctgggaagat tcgccgaagg gatggccgca
     1021 gcggccgacg tatggctgag gcaagcagcg cgaataatcg tcgaatgatg caagcgcggg
     1081 atgaaaaacg aagagcggga gcgtggatcg cgacggcgtt ggcgatcgc
//
LOCUS       DASPLT010000427         2785 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_189948, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000427 DASPLT010000000
VERSION     DASPLT010000427.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 2785)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2785)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2785
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_189948"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..400
                     /locus_tag="VEA63_04440"
     CDS             <1..400
                     /locus_tag="VEA63_04440"
                     /inference="COORDINATES: protein motif:HMM:NF019886.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HYD83269.1"
                     /translation="APTLFAAPVPDPIHVHEMKTLLEQCISQLPRSHRAVYLLREIQH
                     LTTAETAACLGLSRENVKVCLHRAREGLKAQLLKSSAGVELFDYPAQFCDPVTARVML
                     AIHSAWRAARKKAQLHGRADAGSGQQPSAG"
     gene            571..1050
                     /locus_tag="VEA63_04445"
     CDS             571..1050
                     /locus_tag="VEA63_04445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HYD83270.1"
                     /translation="MSRSTENLSKADYEALAEFRYALRKFLGFSEDAASKQGITPQQY
                     QALLAIEGFPGRNWVTIGELAEQMRIAHHSAVGLVDRMEAMKLVKRTTAKEDRRRVRV
                     ALSAKGLKLLAKLYLVHREELRSSGQQLASLLQKAALRIPAKIPLASPACSMPEIED"
     gene            1538..2731
                     /locus_tag="VEA63_04450"
     CDS             1538..2731
                     /locus_tag="VEA63_04450"
                     /inference="COORDINATES: protein motif:HMM:NF013237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HYD83271.1"
                     /translation="MSEPAHRFATLCARAGTQRRDAAANAPVQPPIVQSAIFDLGGTD
                     DAEAIFSGARKGYAYSRFGNPTVDALARTLAVFEGGADALVTSSGNAAVLCAITAAMH
                     GRSGPLVTHHDIYGGSFELIRTLSSVYQLPVEFVDSTRTEQWLHAVSHAGAVLLETPS
                     NPLMRLVDLAQTVEHARIAGAPVIVDNTVATPFNQRPFEFGADWIVHSTTKYLNGHAD
                     LIGGCVIRREPLSNVDRSIHKNLGGTVNATEAAMVLRGLRTFALRMEAHNRNGAALAN
                     WLAARPEVSRVYYPGLPAHPQAELVQRQMTRGAGGLLSFELAGGESAAVALFDRLKLI
                     VHAVSLGGPETLVTRPARTSHRGMTPEARREAGVSDGLIRLSAGFEDINDLIADLEQA
                     IARAT"
BASE COUNT          545 a          936 c          812 g          492 t
ORIGIN      
        1 cgcaccgacg ttgttcgccg cgcccgtccc cgatccgatt cacgtgcacg aaatgaaaac
       61 tctcctcgag caatgcatct ctcaactgcc gcgctcgcac cgcgcggttt acctgcttcg
      121 tgaaatccag catctcacga ctgcggagac cgccgcgtgc cttggccttt ctcgggagaa
      181 cgtcaaagtc tgcctgcacc gcgcgcgaga gggtttgaag gcgcagttgc tcaagagctc
      241 ggccggcgtc gaactcttcg actatcccgc tcaattctgc gatccggtga ccgccagagt
      301 catgctggcg atccattcag cctggcgcgc cgcgcgaaaa aaggcgcaac tgcacggacg
      361 cgccgacgca ggatcggggc agcagccgtc cgccggctaa caaacacgat cgggtgctcg
      421 cggttcgatt cgaggacgcg gtgcaacctg gctgggatgc agcgaacgcc ggccctgaaa
      481 atggcgttgc gcggcgactc gtgctaatat cgcatcgcga tacgatgtcg ttcaattcta
      541 tcaatgctcg ctgaacttcg ctcgcacctc atgtcacgct ccaccgaaaa tctttccaaa
      601 gccgactacg aagccctcgc cgaattccgc tacgcgctgc ggaaatttct agggttcagt
      661 gaagacgccg cctcgaagca aggcattacg ccgcagcaat accaggccct gctcgccatc
      721 gagggcttcc ccgggcgcaa ctgggtgacg atcggcgagc tcgccgaaca gatgcgcatc
      781 gcgcatcaca gcgccgtggg tctcgtcgat cgcatggagg cgatgaaact ggtcaagcga
      841 accaccgcga aggaagaccg gcgacgcgtc cgcgtggcgc tctcggcaaa agggctgaag
      901 ctcctcgcca aactctatct cgtgcaccgc gaggaactcc gctcgtccgg acaacagctc
      961 gcatcgctgc tccagaaagc ggcgttgcgt attcccgcca aaatcccgct cgccagcccc
     1021 gcgtgctcca tgccggaaat cgaggactga aacccgaacg agcggtcgca cgtcgtcgac
     1081 cgtcttgaaa gtatttccgg acgacgcgca cgaagcacat cgggatgctc catctttaat
     1141 cgcggccgca cgcggttcgt taaaaatccg ggcattccgc ttatggcggt ttaagtaaaa
     1201 acatagcttg ttgtaagagc ctgcttgcag gcgacatgat gcgtcgtctg cgctcgatgc
     1261 gcgaatcgcc tgcaagcagg ctcctgcctt catacggaaa aggcacattc ccccctgacg
     1321 ggttttaacg catccgccgg caacaaacgc tcacgcagac gcactaaaat ccgcccgggc
     1381 gcccggccca gtttcgcgcg acgaagcttg ccaaagcccg gtttgtatcg taacacgata
     1441 caatgaaaaa cgggtcgtgc gagttcaacc gctgccagcc gaaccggtcc ggccggaagg
     1501 ccgtggcggc gcgctttcct caacgaaagg gccgctcatg agcgaacccg cgcatcgttt
     1561 tgcgacgctg tgcgctcgcg ccggaactca acgccgcgac gccgccgcga acgctccggt
     1621 tcaacccccg atcgttcaaa gcgcgatttt cgacctcggc ggaacagacg acgccgaagc
     1681 catcttctcc ggggcgcgca aaggctacgc ctactcacgc ttcggcaatc cgaccgtcga
     1741 tgcgctcgcg cgcaccctcg cggttttcga aggcggcgcg gatgcgctgg tcaccagctc
     1801 gggcaacgcc gcggtcctct gcgcgatcac cgctgcgatg cacggccgca gcggcccgct
     1861 tgtcacgcac cacgacattt acggcggcag cttcgagctg attcgcaccc tctccagcgt
     1921 ttatcagctt ccggtggagt tcgtcgattc gacgcggacc gagcaatggc tgcacgcggt
     1981 gtcgcacgcc ggcgcggttt tgctcgaaac gccgtcgaac ccgttgatgc ggctcgtcga
     2041 tctcgcgcaa acggtggaac acgcacgcat cgccggagca ccggtgatcg tcgacaacac
     2101 cgtggccacg ccgttcaacc agcgcccctt cgaattcggc gcggactgga tcgttcattc
     2161 gacgacgaaa tatctcaacg gccacgccga cctgatcggc ggctgcgtga tccgtcgcga
     2221 gccgttgtcg aacgtggacc gttccattca caaaaacctc ggtggcaccg tcaatgcgac
     2281 ggaagccgcg atggtgttgc ggggcctgcg cacgttcgcg ttgcgaatgg aagctcacaa
     2341 ccgcaacggc gccgcgctgg cgaactggct cgcggcgcga ccggaggttt cgcgcgttta
     2401 ctatcccggg cttcctgcac acccgcaggc ggaactcgtg caacgacaga tgactcgggg
     2461 cgcgggcggt ttgctttcct tcgaactcgc cggtggtgaa tcggccgcgg tggcgctctt
     2521 cgaccgattg aaactcatcg tccacgccgt cagtctcggc ggtccggaaa cgctcgttac
     2581 gcgcccggcc cgaaccagtc accgcgggat gacaccggaa gcgcgccgtg aagcaggcgt
     2641 aagcgacggc ctcatccgtt tgtccgccgg gttcgaagat atcaacgatc tcatcgccga
     2701 tctcgagcag gcgatcgctc gcgccacatg aaacgggatg aacgcgccct ccgattttcg
     2761 ccgcgcgaga accgcgcatc tcacg
//
LOCUS       DASPLT010000428         1218 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_210210, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000428 DASPLT010000000
VERSION     DASPLT010000428.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1218)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1218)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1218
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_210210"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..901)
                     /locus_tag="VEA63_04455"
     CDS             complement(<1..901)
                     /locus_tag="VEA63_04455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XylR family transcriptional regulator"
                     /protein_id="HYD83272.1"
                     /translation="MAVVTTTASKALGDDGAGGLALASVSAAFQVPRVKPHVLLVFMT
                     RFEECAAMLKGIAQYERSHRPWAAFLDDEALAETSEQWLRSKKWHGVISRHTTPALMK
                     ICAELKLPLVDLNDTEPFPGVPKIRPDNVSIGHVGAEHLLERGFRHFGFCGFNDQGWS
                     GERRDGFVEALSLAGHRCDLLEVEQPEDVTPFWDAKQIPVLSGWLKRLPKPAAVMACN
                     DLRALQIVSAAQAAGLLVPEEVAVLGANNDTIRCELAEPPLSSVATNAFQSGYQAAEK
                     LALMMAGQEPGDLNRRVEPLGVIT"
     gene            complement(946..>1218)
                     /locus_tag="VEA63_04460"
     CDS             complement(946..>1218)
                     /locus_tag="VEA63_04460"
                     /inference="COORDINATES: protein motif:HMM:NF024742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HYD83273.1"
                     /translation="GAFTACGSWIMKVGMAVGAGASGVILSLTGFDADLGGAQADFTI
                     FMIRLLLAAIPIAGILLALAMIYFFPLTQEKMAEIRRELEARRGTV"
BASE COUNT          241 a          456 c          314 g          207 t
ORIGIN      
        1 gtgtgatcac gccgagcggc tccacccgac ggttcaaatc gcccggctcc tgtccggcca
       61 tcatgagcgc cagcttctcc gccgcctgat acccggattg aaacgcattc gtcgccacgc
      121 tggataacgg cggctccgcc agttcgcagc gaatcgtgtc gttgttcgcc cccaacaccg
      181 ccacctcttc cggcaccagc agacccgcgg cctgcgccgc gctcacgatc tgcagcgcgc
      241 gcagatcgtt gcacgccatc accgccgccg gttttggcag acgcttcagc cagccactca
      301 ataccgggat ctgcttcgcg tcccaaaacg gcgtcacgtc ctccggctgc tccacctcga
      361 gcaagtcgca acggtgaccc gccagactca acgcctcgac aaatccgtcc cggcgctcgc
      421 ccgaccagcc ttggtcattg aacccgcaaa acccgaaatg acggaatccg cgctcgagca
      481 aatgctccgc accgacgtgg ccgatcgaca cgttgtcagg ccgaattttc ggcaccccgg
      541 gaaaaggttc cgtgtcgttc aaatccacca gcggcaactt caactccgcg caaattttca
      601 tcagcgccgg cgtcgtgtgg cgactgatca cgccgtgcca tttcttgctg cgcagccatt
      661 gctcgctcgt ctccgccagc gcctcgtcgt caaggaaagc ggcccacgga cgatgcgatc
      721 gttcgtattg ggcgatccct ttcaacattg ccgcgcattc ctcgaagcgg gtcatgaaga
      781 caagcagcac atgcggtttc acgcgcggaa cttgaaaggc cgccgaaacc gatgcaagcg
      841 cgaggccgcc cgcgccgtcg tcgcccaaag ctttgctcgc ggtggtcgtc acgaccgcca
      901 tcgttcaacc ttcgctgccg cgccgacgca cacttcgcca caccctcaca ccgtgccgcg
      961 acgcgcctcg agctctcggc gaatctccgc cattttctcc tgcgtcagcg ggaaaaaata
     1021 gatcatcgcg agcgccagca aaattcccgc gatcggaatc gccgccaaca gcagccgaat
     1081 catgaaaatc gtgaagtccg cctgcgctcc gcccagatcc gcgtcgaaac cggtcagcga
     1141 aagaatcacg cccgacgcgc cggcccccac cgccatgccg actttcatga tccacgaacc
     1201 gcacgcagtg aacgcgcc
//
LOCUS       DASPLT010000429         1789 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_251074, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000429 DASPLT010000000
VERSION     DASPLT010000429.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1789)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1789)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1789
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_251074"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..250
                     /locus_tag="VEA63_04465"
     CDS             <1..250
                     /locus_tag="VEA63_04465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD83274.1"
                     /translation="DRLHLLTYGEFEKQLRDIDLPMVALHKYPAYNYGWSMPSFRTQT
                     NEELHQWLGLLQRYFVIFYEDADFIILTRPHLVPLLAR"
     gene            269..850
                     /locus_tag="VEA63_04470"
     CDS             269..850
                     /locus_tag="VEA63_04470"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HYD83275.1"
                     /translation="MKTNVAILIFDDVEVLDFAGPFEVFAVADELHDHHAFHTFTLGL
                     KPGTVRARNGLKVVPDFTLENCPPPQIIIVPGGQGRRALMQMPALHEWLRQKSRRAEI
                     VMSVCTGAFVLARAGLLDGLRVTTHHLSIAELREFAPQLDVVENERFTDNGKFVTAAG
                     ISAGIDSSLHVVARLLGDDAADRTAAHMEYRRA"
     gene            920..>1789
                     /locus_tag="VEA63_04475"
     CDS             920..>1789
                     /locus_tag="VEA63_04475"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HYD83276.1"
                     /translation="MTASPPSPTRFALILAFASIYVIWGSTYLGIRVAIETMPIFLMA
                     AARFLLAGAALFLFLRARGAPTPTARQWGVNTVIGTFLLLGGNGLVAWAEQFVPSGVT
                     ALIIGVQPLFFVLTEWAWPGGMRPTLTTSAALLLGFAGVAWLAAPWENSAHGGLHVGG
                     VTAILAACIFWAIGSIYSRHAKHGADPLLASSLQMLGGGGVLTLAALFHGDFAQLDIA
                     AISARSWTAFAYLVLFGSLIGFSTFVWLMKHSTPARVSTYAYVNPLVAVFLGWLILDE
                     PVTSRTFVAAVLIV"
BASE COUNT          275 a          656 c          487 g          371 t
ORIGIN      
        1 ggatcggctg catttgttga cctacggcga gttcgagaag cagcttcgcg acatcgatct
       61 gccgatggtc gcgctgcaca agtatccggc ctacaattac ggttggtcga tgccgtcgtt
      121 ccgcacccaa accaatgagg aacttcacca gtggctcggc cttctccagc gctacttcgt
      181 catcttctac gaggacgcgg atttcatcat ccttacgcga cctcacctcg ttccgctgct
      241 ggcccgctga attctctgaa accgccgtat gaagacaaac gttgccatcc tcatcttcga
      301 cgacgtcgaa gtgctcgact tcgccggacc ttttgaagtt ttcgccgtcg ccgacgaact
      361 ccacgatcac cacgcgtttc acaccttcac gctcggcctc aaacccggca ccgttcgcgc
      421 gcgcaacggc ctgaaggtcg tgcccgactt cacgctcgag aactgtccgc cgccgcaaat
      481 catcatcgtg ccaggcggcc agggccggcg cgcgctcatg caaatgcccg cgctgcacga
      541 atggctccgc caaaaatccc gccgcgccga gatcgtgatg agcgtgtgca ctggcgcgtt
      601 cgtgctcgcc cgcgccggtt tgctcgacgg acttcgcgtg acgacgcacc acctcagcat
      661 cgccgagttg cgcgaattcg caccgcaact cgacgtcgtc gaaaacgagc gcttcaccga
      721 caacggaaag tttgtcaccg cggccggcat ctccgccggc atcgatagct cgctgcacgt
      781 cgtcgcacgc ttgctcggcg acgacgccgc ggatcgcacc gccgcgcaca tggaatatcg
      841 ccgcgcctaa ttttcacggt agcacgggca acctgcccgt gctgtcgcgc tatccatcgc
      901 tcgcgccacc gttttcaccg tgaccgcctc cccgccctca cccacgcgct tcgctctcat
      961 tcttgcgttc gcctcgatct acgtgatctg gggcagcacg tatctcggca ttcgcgtcgc
     1021 gatcgagacg atgccgatct tcctgatggc ggccgcgcga ttcctcctcg ccggcgcagc
     1081 gctgtttttg tttctccgcg cccgcggcgc tccaacgccc accgctcgcc aatggggcgt
     1141 caacaccgtc atcggcacgt ttctcctcct cggcggcaac ggtctcgtcg cctgggccga
     1201 gcaattcgtg ccatcgggcg tcaccgcgtt gatcatcggc gtgcaaccgc tctttttcgt
     1261 gctcaccgaa tgggcgtggc ccggcggcat gcgacccacg ctgaccacct ccgccgcgct
     1321 gctcctcggc ttcgccggcg tcgcctggct cgcggcaccg tgggaaaaca gcgcccacgg
     1381 cggactccac gtcggcggag tgaccgcgat tctcgccgcc tgcatcttct gggcgatcgg
     1441 ctcaatctac tcgcgccacg cgaaacacgg cgccgatccg ctgctcgcgt cttcgctgca
     1501 aatgctcggt ggcggaggcg tgctcacgct cgccgcgctc ttccacggcg atttcgcgca
     1561 gcttgatatt gcggcgatct ccgcgcgatc ctggaccgct ttcgcctatc tcgtgctgtt
     1621 cggctcgctc attggattct cgaccttcgt ctggctgatg aaacacagca cgcccgcgcg
     1681 cgtgtcgacc tacgcttacg tgaacccgct cgtcgccgtt ttccttggct ggctgatcct
     1741 cgacgagccg gtgacgtccc gcaccttcgt cgccgcagtt ctcatcgtc
//
LOCUS       DASPLT010000430         1064 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_169743, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000430 DASPLT010000000
VERSION     DASPLT010000430.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1064)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1064)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1064
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_169743"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(72..>1064)
                     /locus_tag="VEA63_04480"
     CDS             complement(72..>1064)
                     /locus_tag="VEA63_04480"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HYD83277.1"
                     /translation="RASAMPERNPNELTTEQGYKLLDEIRSFGEPLMVFTGGDPLKRP
                     DLFDLIRHSVKIGLRTNVTPSATPLLTEAAIDGFKAAGVTRMAISVDGHDAPSHDDFR
                     GIPGTFDRAMLALRHAQKIGLETQFQTTVTRRNMANLPKIADIAADVGTKMWSLFFLI
                     VTGRAGEADDLTADEYEKVFAFMYSLSKTAPFAIKTTEAMHYRRYVAQRMRDEHGPRP
                     ETEGARQVAFRTAGVSDGKGFVFVSHTGEVFPSGFLPVSGGNVLRDPLTSVYRDSDLF
                     RRLRDTSLREGKCGICEYVKVCGGSRSRAYALTGDYLASDPRCVYQPHVAEALI"
BASE COUNT          182 a          329 c          353 g          200 t
ORIGIN      
        1 aatgccgtcg tgtggttccg gtcaaaccct tcctgtgcgt ccaacctgcc gcaatacgaa
       61 atgcgaaggc cctatatcaa tgcttcggcc acgtgcggct ggtagacgca gcgggggtcc
      121 gacgcgagat aatctccggt cagcgcgtac gctcgcgacc gcgagccgcc gcaaaccttg
      181 acgtactcgc aaatgccgca cttgccctca cgcaggctgg tgtcgcgaag ccttcggaac
      241 aggtccgaat cccggtagac gctggtgagc ggatcgcgca agacgttgcc gccggaaacc
      301 ggaagaaaac cgctcgggaa gacctcgccc gtgtgggaga cgaaaacgaa tcctttgccg
      361 tcgctcacgc cggcagtccg gaacgccacc tgccgggcgc cttccgtctc gggacgcgga
      421 ccgtgctcgt cgcgcatgcg ctgcgcgacg tagcggcgat aatgcatggc ttccgtcgtt
      481 ttgatggcga agggcgcggt tttcgagagg ctgtacatga acgcgaatac cttctcgtac
      541 tcatccgccg tgaggtcgtc ggcttcgccg gcacggcccg tgacgatgag gaagaagagg
      601 ctccacatct tcgtccccac atcggcggca atgtcggcga ttttcggcag attcgccata
      661 ttgcgccgcg ttacggtggt ttggaactgc gtttccaggc cgatcttctg cgcgtgccgc
      721 agcgccaaca tggcgcggtc gaaggtgccg gggatgccac ggaaatcgtc gtggctgggg
      781 gcatcgtggc cgtccacgct gatcgccatg cgggtgacgc ccgctgcttt gaagccgtcg
      841 atggcggcct cggtgagcag cggcgtggcg ctgggcgtca cgttggtccg aagcccgatc
      901 ttcacactgt ggcggatcag gtcgaaaagg tccggccgct tcaaggggtc gccgccggtg
      961 aagaccatga gcggctcccc gaaggagcgg atctcgtcga gcagcttgta cccctgctcg
     1021 gtggtgagtt cattggggtt ccgttcgggc atcgccgacg cgcg
//
LOCUS       DASPLT010000431         1161 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_148475, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000431 DASPLT010000000
VERSION     DASPLT010000431.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1161)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1161)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1161
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_148475"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..442)
                     /locus_tag="VEA63_04485"
     CDS             complement(<1..442)
                     /locus_tag="VEA63_04485"
                     /inference="COORDINATES: protein motif:HMM:NF024923.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HYD83278.1"
                     /translation="MPSKPIFLFALLPLLLAACSREQPAASAPPPPAVTAVTVASAPV
                     TLTRELPGRSAPFLVAEVRPQVTGIVQKRLFDEGERVEEGQPLYQLDDSTYRADFQRA
                     KATLARARATAELARVNARRAANLAELDAVSRQENETAIATLAQA"
     gene            complement(632..793)
                     /locus_tag="VEA63_04490"
     CDS             complement(632..793)
                     /locus_tag="VEA63_04490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD83279.1"
                     /translation="MNAKGYTGIAVGIAIGVAIGAMMDNVAMGIGIGIALGIAMMSLF
                     KTPGDGGRR"
     gene            complement(856..>1161)
                     /locus_tag="VEA63_04495"
     CDS             complement(856..>1161)
                     /locus_tag="VEA63_04495"
                     /inference="COORDINATES: protein motif:HMM:NF021497.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A-modifier LpxR family protein"
                     /protein_id="HYD83280.1"
                     /translation="AATNSPLDDRDPRLGAGSRTSLFVFGGVDGRAVARDIFLDGNTW
                     EDSPSVDKETWVGDSYWGVGIVRGGWQFTYTYVVRSKEFEAQREVNQFGSITISRAF"
BASE COUNT          221 a          403 c          330 g          207 t
ORIGIN      
        1 cggcctgcgc caacgttgcg atcgccgtct cgttctcctg ccggctcacc gcgtcgagtt
       61 cggccaggtt tgccgcgcgg cgcgcgttga cccgcgccag ctccgcggtg gctcgcgcgc
      121 gcgccagcgt cgcctttgca cgctggaaat ccgcgcgata cgtcgagtcg tccaattgat
      181 acaaaggctg cccctcctcc accctctcgc cttcgtcgaa caatcgcttc tgcacgatgc
      241 cggtgacctg tgggcgcacc tccgcgacga ggaatggcgc cgaccggccc ggcagctctc
      301 gcgtgagcgt cacgggagcg gatgcgaccg tgacggcggt gacggcgggc ggcggcggag
      361 cggaagcggc cggttgctca cggctgcacg cggccagcaa cagcggaagc agagcaaaga
      421 ggaagatcgg tttcgaaggc ataaaacaca aaggggaccg aaggagatga cgttcgattt
      481 cggaaaatag cactacattg ccgacgactg aactgttttg tcgtgtccct actgatggcc
      541 gtcatcggcc gcttccccat cgctccgcct cttttctaaa aaacgggcgc cagccttcgt
      601 tttgcggaat acgaatgctt tccgatcggt atcagcgcct gcccccatcg cccggcgttt
      661 tgaagagaga catcatcgcg atgcccagcg cgatcccgat gccgatgccc atggcgacgt
      721 tgtccatcat ggctccgatg gccacgccga tagcaattcc gacggcgatt cctgtgtagc
      781 ctttcgcatt cacccgctcg attgctaaac cagaaacacc cctcgcgcaa acgccggcgc
      841 cgccgcccgc gcaactcaaa acgcgcgcga gatggtgatg gagccaaact gattcacttc
      901 gcgctgcgcc tcgaactcct tcgaacgcac cacatacgtg taggtaaact gccacccgcc
      961 gcgcacgatc cccacgcccc aatacgaatc acccacccac gtctccttgt cgacgctcgg
     1021 gctgtcctcc cacgtgttgc cgtcgaggaa aatatcgcgt gccacggcgc gtccatcgac
     1081 gcccccgaaa acaaataagc tagtgcgcga tcctgctccc aagcgcggat cgcggtcgtc
     1141 gagcggcgaa ttcgtcgccg c
//
LOCUS       DASPLT010000432         3642 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_66086, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000432 DASPLT010000000
VERSION     DASPLT010000432.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 3642)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3642)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3642
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_66086"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..815)
                     /locus_tag="VEA63_04500"
     CDS             complement(<1..815)
                     /locus_tag="VEA63_04500"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HYD83281.1"
                     /translation="MIKPLSAPARYAVLAAAFGALLFDGFELGLMPLASLSVTQDLLG
                     DAYTPTIGGDWFARYTAALMFGAAVGGILLGSLGDRIGRTRAMAVSILFYAVFAGLGA
                     YVTNQWQMLALRFVVGLGVGGVWPNAVALVSECWPDKAKPTVAGLMGAAINTGILLLS
                     QIAQQWNVAPESWRWLFHVAAFPAVLGLLVLFFLPESPNWLAARQEQRHQKKRAAPLR
                     ELFQPQLIRITLTGILLGSIPLIGAWAASKWMIPWADKIGGEMHVDYKAMTQG"
     gene            complement(1067..2152)
                     /locus_tag="VEA63_04505"
     CDS             complement(1067..2152)
                     /locus_tag="VEA63_04505"
                     /inference="COORDINATES: protein motif:HMM:NF024770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HYD83282.1"
                     /translation="MKAPPTVRDIARVADVAVGSVSRALNGHPDLNADLRERILNAAR
                     ELGYTPLRKRRTNSQPRRGGLGEGGHIGLICFGMEDTLVQLPVVSAAVHGIEEAVSLS
                     GGTLMFASIPRGDRLPPFLAEKQVSGVILKGPNMGLLPPESESELLRAIYRLPHVWLL
                     GQLPNARGDHCNFDHEIAGRLAANHLREKGHTRVAFLNPKPGHAQFEPLKSAFIYSAL
                     QQGMKVDVLEPASDKLISWPLPAVASEPVVEALVSEWAKTRPKQRPTVIAVGADSTAV
                     QLYAALNKIGVRPGKDVGIISCNDERSLIMSLNPSLTTINVGAEMVGRNAVARLAWRL
                     AHGNDDNSVRILVEPKLVVRDSVPQLK"
     gene            complement(2366..2710)
                     /locus_tag="VEA63_04510"
     CDS             complement(2366..2710)
                     /locus_tag="VEA63_04510"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HYD83283.1"
                     /translation="MSKNYSITHLNDIAPVKCPCGFSKRAFATPDNQVATIHLVDIQE
                     DAQTHYHKKLTEIYLILEGEGFMELDGELHPVKPMSTILIKPGCRHRAIGKLKIINVP
                     VPAFDPADEWFD"
     gene            complement(2759..>3642)
                     /locus_tag="VEA63_04515"
     CDS             complement(2759..>3642)
                     /locus_tag="VEA63_04515"
                     /inference="COORDINATES: protein motif:HMM:NF045341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD83284.1"
                     /translation="ELFARTVEAAELLGVDAELAQRLRGQLERIAPYRIGARGQLQEW
                     REDYVEHDPAHRHLSHLYGFHPGNQINADATPELMRAVRRTMELRGDEATGWSMGWKV
                     NLWARMMDGDHAYKIIRNFFHLVESTEVGMTGGGLYPNLFDAHPPFQIDGNFGYTAGV
                     AEMLVQSHAGVVQLLPALPSAWPAGAVKGLRARGGFEVDLSWDEGKLTEATIRSKVGG
                     VLRLRTPEKAAVEISGGVSGPAEGANPNPFFRIVPAGRPEVAASAEQAAIAMTLPGTR
                     TVDVETVAGGVYHFRAR"
BASE COUNT          734 a         1298 c          970 g          640 t
ORIGIN      
        1 cacccctgcg tcatcgcctt gtagtcgacg tgcatctcgc cgccgatttt gtccgcccac
       61 ggaatcatcc acttgctcgc cgcccacgcg ccgatcagcg gaatcgatcc cagcaaaatt
      121 ccggttaacg tgatccgaat caattgcggc tggaaaagct cccgcaacgg cgcggcccgc
      181 tttttctggt gcctctgttc ctgccgcgcc gcgagccagt tcggcgactc cggcaggaag
      241 aacaacacca gcagaccgag caccgccgga aacgccgcaa cgtgaaacag ccaccgccag
      301 gactccggcg cgacgttcca ctgctgcgca atctgcgaaa gcagcagaat cccggtattg
      361 atcgccgctc ccatcaggcc ggccaccgtt ggcttcgcct tgtcgggcca gcactccgag
      421 accagcgcga ccgcattcgg ccagacgccg cccacgccca gtccaaccac aaaacgcaac
      481 gccagcatct gccactgatt cgtcacgtag gcgcccaacc ccgcaaacac cgcatagaat
      541 aaaatactca ccgccatcgc ccgcgtccgc ccaatgcgat cccccaagct acccaacagg
      601 ataccaccca ccgccgcgcc gaacatcaga gccgccgtgt aacgtgcgaa ccaatccccg
      661 ccgatcgtcg gcgtgtaagc gtcgccgagg agatcctgcg tcaccgaaag cgaagcgagc
      721 ggcatcagcc cgagttcgaa accgtcgaag agcagcgcgc caaaagccgc ggccaacacg
      781 gcatagcggg cgggggcaga gaggggtttg atcatgtgac gtggcggggg taacgcagcg
      841 caacagccct ccttcaggat cgtgaaatcc caaaaggatt ggcgcgccgc agtgacaggg
      901 gcatgaacgc gtcggacgac ttagttcatg aaatttctat gaacaaaaat gaagcctgcg
      961 cggccgctgt caaaccgcga tcgctgccag cggcgaattt ccttcgtaaa ccagcaaatt
     1021 gccggcaatg cgccacggcc gaacttcccg atgaacaaaa atgaacttac ttcagctgcg
     1081 ggaccgaatc gcgcacgacc agcttcggct cgacgagaat cctgaccgag ttgtcgtcgt
     1141 tgccatgcgc caggcgccac gcgagccgcg ccaccgcatt tcttccgacc atctccgcgc
     1201 ccacgttgat ggtcgtgagg cttggattca ggctcatgat cagggaccgc tcatcgttgc
     1261 aggaaatgat cccgacatcc ttgccgggcc ggacgccaat cttgttcaac gcggcgtaga
     1321 gctgaaccgc cgtcgagtcg gcacccacgg caataaccgt cggccgttgc ttgggacgcg
     1381 tcttcgccca ttcactgacg agcgcttcca ccacgggctc cgatgccacc gccggcaacg
     1441 gccacgaaat cagcttatcg ctcgccggtt cgagcacgtc caccttcatg ccctgctgga
     1501 gcgccgagta gatgaacgcg gacttgagcg gctcgaactg cgcatgcccc ggctttggat
     1561 tcaggaacgc cacgcgtgta tgccccttct cccgcaggtg attcgcggcg agacggcccg
     1621 caatttcatg atcaaaatta caatgatcac cgcgcgcgtt cggcaattgt ccgagcagcc
     1681 agacgtgcgg aagccgatag attgcccgca acaattcact ttccgattca ggcggcaaga
     1741 gccccatgtt cggccctttc aggatcacac ccgacacctg cttttccgcc aggaacggcg
     1801 gcagccggtc gccccgcgga atgctggcga acatcaaggt gccgccgctg agcgagaccg
     1861 cctcttcgat gccgtgcacc gccgcgctca ccaccggcag ttgcaccagc gtatcctcca
     1921 taccgaagca aatcaggccg atgtgccctc cctcgccgag tccgccgcgt cgaggctgcg
     1981 agttcgttcg ccgcttccgc aggggcgtat acccgagttc gcgcgccgcg ttcaggatgc
     2041 gctcacgcag gtcggcattc agatccgggt gcccgttgag cgcccgcgat accgaaccca
     2101 ccgcgacgtc cgcgacccgc gcgatatcgc gcaccgtcgg cggagctttc attatccgag
     2161 ttgcttttgc tgccacagaa agacgctgac agaaccctgg actacttacc atcaaatccg
     2221 cgcaagttca tggaatccgc ttccgggccg tgaactaacc gggccgcaat gctccacgca
     2281 tttcccgacg tcggcccaag tgccccaccc catcgctctc gcgcccgacg accgtttccg
     2341 cgccgcccga cgcacgccag ctcgctcaat caaaccactc gtccgccgga tcgaaggcgg
     2401 gcaccggcac attgatgatc ttcaacttcc ctatcgcacg gtgccggcag ccgggcttga
     2461 tcaaaatggt cgacatcggc ttcacgggat ggagttcgcc gtcgagttcc atgaaccctt
     2521 cgccttccag aatcagataa atttccgtca gcttcttgtg atagtgcgtc tgcgcgtcct
     2581 cctgaatgtc caccagatga atcgtcgcga cttgattgtc gggcgtcgca aacgcccgct
     2641 tggaaaagcc gcacgggcat ttcaccggcg cgatatcgtt cagatgggtg atcgagtagt
     2701 ttttgctcat gatgaaaaaa agtaagcgcc acgtttcgcg ttcggtcgtt cgcagtgatc
     2761 agcgcgcgcg gaaatgataa acgccgcccg cgacggtctc aacgtccacc gtgcgcgttc
     2821 ccggaagcgt cattgcgatt gctgcctgct ctgcgctcgc agcgacctcc ggcctcccgg
     2881 cggggacgat ccggaaaaac ggattcggat tcgcgccttc cgctggcccg ctcacgcctc
     2941 cggagatttc caccgccgcc ttctccggcg tccgcagccg cagcacgccg cccaccttcg
     3001 aacgaatcgt cgcctccgtc agcttccctt cgtcccagct caaatccact tcgaaaccgc
     3061 cgcgcgcgcg taatcctttt accgcgccag ccggccaggc cgacggcaac gccggcaaga
     3121 gctgcaccac gcccgcatgg ctttgcacca gcatctccgc cacgcccgca gtgtagccga
     3181 aattcccgtc gatctgaaac ggcggatgcg catcgaacaa attcggatag agtccgcctc
     3241 ccgtcattcc gacctcggtg gattccacca ggtggaagaa attacggatg atcttatacg
     3301 catggtcgcc atccatcatg cgcgcccaga ggttcacctt ccaccccatc gaccaacccg
     3361 tggcctcgtc cccgcgcaac tccatcgtcc gccgcaccgc acgcatcaac tcgggcgtcg
     3421 catccgcatt gatctgattg ccgggatgaa atccgtagag atgcgagaga tgccggtgcg
     3481 ccggatcgtg ctccacatag tcctcccgcc actcctgcaa ctgcccgcgc gccccgattc
     3541 gatacggagc gattcgctcc aactgcccgc gcaaccgctg cgccaactcc gcgtccaccc
     3601 caagcagctc cgccgcctcc accgtgcgcg cgaacagttc cc
//
LOCUS       DASPLT010000433         1760 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_66122, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000433 DASPLT010000000
VERSION     DASPLT010000433.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1760)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1760)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1760
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_66122"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(69..1241)
                     /gene="metK"
                     /locus_tag="VEA63_04520"
     CDS             complement(69..1241)
                     /gene="metK"
                     /locus_tag="VEA63_04520"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HYD83285.1"
                     /translation="MANTFVFSSESVGEGHPDKVADLISDSVLDACLAQDRTSRVACE
                     TMVKSNMVILAGEITTRAKLNYEAIVRAAIRDIGYVNNDDVFHADSVFINNYLTRQSP
                     DIAQGVDAKKAKGKKTAEQGAGDQGIMFGYACSETPELMPTPIIFAHRLGRELTKVRK
                     SGAVKWLRPDAKSQVSIRYENDKPVEVVNVVISTQHTEDVQHAQIEKYLIENVVKKVI
                     PASMLTKKTEYLINPTGRFVVGGPQGDSGLTGRKIIVDTYGGWGRHGGGAFSGKDPSK
                     VDRSAAYMGRWVAKNIVAAGLATHTEIQFAYAIGHPQPVSVRVETFGTAKLGISDEQI
                     TAAVQKVFSFKPADIVSQLNLLRPIYRQTTNYGHFGKSGLPWEKTDKAAALRAAIK"
     gene            1438..>1760
                     /locus_tag="VEA63_04525"
     CDS             1438..>1760
                     /locus_tag="VEA63_04525"
                     /inference="COORDINATES: protein motif:HMM:NF013767.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009927 - histidine phosphotransfer kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HYD83286.1"
                     /translation="MADTPVIDLQSIENLRSLNPDDNDEFLREIVTIYLEDTPQRIAE
                     LEQALGVADVAKFTRAAHSVKGSSSNVGAMVVRNVAEQLEHQSKQGLDDVASLLASLK
                     IEFARA"
BASE COUNT          347 a          555 c          498 g          360 t
ORIGIN      
        1 tggcgtcttt ggtggcagtg ggcattgtat tcagaatttg gatgttacgg ttgacggcgc
       61 tcggccggtt acttgatggc tgcgcgaagc gcggcagcct tgtcggtctt ttcccacggc
      121 aatccggatt tgccgaagtg accgtagttg gtggtctggc gataaatcgg gcgcagcaga
      181 ttgagctgcg acacgatgtc agccggcttg aagctgaaaa ctttttggac ggccgccgtg
      241 atttgttcgt cggaaatacc cagctttgcc gtgccgaacg tctccacgcg caccgacacc
      301 ggctgcggat gaccgatcgc gtaggcgaac tgaatttccg tgtgcgtcgc gagcccggcg
      361 gccacgatgt tcttcgccac ccagcggccc atgtaagccg cggaccgatc gaccttcgac
      421 ggatccttgc cggaaaacgc gccgccgccg tgacggcccc aaccgccgta ggtatccaca
      481 atgatcttac ggccggtcag acccgaatcg ccctgcggac cgccgacgac gaaacggccc
      541 gtcggattga tcaggtattc ggtcttcttg gtcagcatcg aggccgggat gaccttcttg
      601 acgacgtttt cgatgagata cttttcgatc tgcgcgtgct gcacgtcctc cgtgtgctgc
      661 gtggagatga ccacgttcac cacctcgacc ggcttgtcgt tctcgtagcg gatcgacacc
      721 tgagacttcg cgtccggacg gagccacttc acggcgccgc tcttgcggac cttcgtcaac
      781 tcgcggccga gacgatgcgc gaaaatgatt ggcgtcggca tcaactccgg cgtctcgctg
      841 catgcgtagc caaacatgat gccctggtcg cccgcgccct gctccgccgt cttcttaccc
      901 ttggcctttt tcgcgtcgac accctgcgcg atgtccggcg actggcgcgt gagatagttg
      961 ttgatgaaca ccgaatcggc gtggaacacg tcgtcgttgt tcacataacc gatgtcgcga
     1021 atggccgcgc gcacgatcgc ttcgtagttc aacttcgcgc gcgtcgtgat ctcgccggcg
     1081 aggatcacca tgttcgactt caccatcgtt tcgcacgcga cacgactcgt gcgatcctgc
     1141 gcgaggcagg cgtcgagcac gctgtcggaa atcaaatccg ccaccttgtc cgggtgacct
     1201 tcgccaacgg actcggaaga gaaaacaaat gtattggcca tatgggtgac ccgtgagaaa
     1261 agcagccgtc cgccgtcaag caagtcgaat atcaacgtat tccgattcgg tgatgcaaaa
     1321 tttggcaagg gatttacacc tcatcacgat agccgaaacg cacctcgcca gcccggagcg
     1381 ctttcaaacg cggcacattc tgtttgcggc gagcgacggc atccaaaaag tcataccatg
     1441 gccgacaccc ctgtcatcga cctgcaatcg attgagaacc tccgctcgct gaaccccgac
     1501 gataacgacg aattcctccg cgagatcgtc acgatctatc ttgaggacac gccgcaacgc
     1561 atcgccgaac tcgagcaagc cctcggcgtc gccgacgttg cgaagttcac ccgggccgcg
     1621 cacagcgtca aaggcagctc ctccaatgtc ggcgcgatgg tcgttcgcaa cgtcgccgag
     1681 caactcgaac accagtcgaa acagggcctc gacgacgtcg ccagcctgct cgccagtttg
     1741 aaaatcgagt tcgcccgcgc
//
LOCUS       DASPLT010000434         1261 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_87269, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000434 DASPLT010000000
VERSION     DASPLT010000434.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1261)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1261)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1261
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_87269"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            31..>1261
                     /locus_tag="VEA63_04530"
     CDS             31..>1261
                     /locus_tag="VEA63_04530"
                     /inference="COORDINATES: protein motif:HMM:NF024829.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HYD83287.1"
                     /translation="MNYAREAVACERVSLFVASNYDRVLADEAVIGELEYEFHLQACS
                     GLKKPHPKSEQALTLQRVAHKLTQLSMTKAATETAEQNASAATPALPATEVNGQPKTL
                     ANSPSAAASPTRPRTQLTLMMRDPSKTPTRPAEVNEYFDLMPMNWATVIPLFDRNQRI
                     CGILLFEGVKLEEKLANSLKPMLDLATSAGRALGTTLYFNQHRSMRIARKVVSLRQHY
                     INTPAKRKWLRFGLPVVLFAALMALPIDYTIKGNASVLAVRQNSLPALVGSRLLEVAV
                     REGEQVKAGQVLARFDTHDIQLQLSQLEQEYERSLLESDAALNMGDEAQMQISRLNAA
                     KAAAMADKLRLDLARAVVRAPFDGLVLGAQTLSTRIGEVLRLGEPALQVVDPTAWQVK
                     AMLRERDLIFLDQFLAES"
BASE COUNT          271 a          409 c          345 g          236 t
ORIGIN      
        1 gtcgaactcg aatcagtcgg ctggaatctg gtgaactacg cacgtgaagc cgtcgcttgc
       61 gaacgcgtca gcctgttcgt cgccagcaat tacgatcgcg tcctcgccga tgaagccgtg
      121 attggcgaat tggagtatga atttcacctc caagcctgca gcggcctgaa aaaaccgcat
      181 ccgaaaagcg agcaagccct gacgctgcaa cgcgtcgccc acaagctcac gcagctctcg
      241 atgaccaagg cggccacgga aaccgccgag caaaacgcct ccgccgcgac gcccgcgctt
      301 cccgccaccg aggtgaacgg ccagccaaag acgttggcta attccccgtc cgccgccgcc
      361 tcaccgacac gccctcgcac tcaattgacg ttgatgatgc gcgatccttc gaagacgccc
      421 acccggccgg ccgaggtaaa tgaatatttc gacctcatgc cgatgaattg ggcaacggtc
      481 atcccgctct tcgatcgaaa tcagcgaatc tgcggcatcc tgctattcga gggcgtcaag
      541 ctggaggaga aactcgccaa ctccttaaag cccatgttgg acctcgccac atcggccgga
      601 cgagcgctcg gcaccacgct ctatttcaac caacaccgct cgatgcgaat cgcgcgaaaa
      661 gtggtttcgc tgcgccagca ttacatcaac acccccgcga agcggaagtg gctgcgcttt
      721 ggtttgccgg tcgtgttgtt cgcagccttg atggcgctcc cgatcgacta cacgatcaag
      781 ggcaatgcca gcgtgctcgc ggtgagacaa aactctttgc cggcactcgt cggttctcgt
      841 ctcctggaag tcgccgttcg cgaaggcgaa caggtgaaag ccggacaagt tttagcccgc
      901 ttcgacaccc acgatattca acttcagctt tcgcaactcg agcaggaata tgagcgctcg
      961 ttgctcgaaa gcgacgcggc tctgaacatg ggcgacgaag cccagatgca gatctcgcgc
     1021 ctcaacgcgg cgaaagcagc agcgatggcc gacaagctac gtctcgacct tgctcgcgcc
     1081 gtcgttcgcg cgcccttcga tggcttggtg ctgggcgccc aaaccctttc aactcgcatc
     1141 ggagaagtcc ttcgcttggg ggaacctgcc ttgcaagtcg tggaccccac cgcgtggcag
     1201 gtgaaagcga tgttgcgcga gcgcgacctg atcttcctcg atcaattcct tgcggaaagc
     1261 g
//
LOCUS       DASPLT010000435         1309 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_66150, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000435 DASPLT010000000
VERSION     DASPLT010000435.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1309)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1309)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1309
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_66150"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(46..>1309)
                     /locus_tag="VEA63_04535"
     CDS             complement(46..>1309)
                     /locus_tag="VEA63_04535"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HYD83288.1"
                     /translation="DDTGAVFFDVNNDGHPDLLIAAGGIEREVGDPLLNDRLYLNDGR
                     GNFATTDGALPADGHSTASIAVADFDSDGKTDLFVGGRVVPGKYPETPRSFLYRNTGG
                     KFVDVTDELAPGLREIGMVTAASFADLDGDQKPDLVLALEWGAITYLRNTGKTFENVT
                     EKSGLAAHTGWWSALTVADVNADGRPDLVAGNVGLNTKYHASAEEPAVLYAGDLDGSG
                     KSQLIEAHYENGKLYPVRGRSKLSYSYPWLTKKFPTFKAYAQATIGDIFSPEKLTSAQ
                     KLTATELASGIFLQQSDGTFAFEPLPRYAQIAPINTIVAHDLDGDGATDLYCAGNNFA
                     PEPNTGRFDGGLGVLLKGDGQGDFTPLTPAESGLLVTGDARSAAALTLGSAKTPALVV
                     ARCEGPLLLFTAKQAKPVATVSKVSSVN"
BASE COUNT          201 a          345 c          512 g          251 t
ORIGIN      
        1 gtgggacacc accgaccggc ggagggcggg ttggagacgc cctgcctagt ttacgctgct
       61 caccttcgaa acggtggcga cgggtttcgc ctgcttcgca gtgaagagga ggagcggccc
      121 ttcgcagcgg gcgacgacga gcgcgggcgt tttcgcggag ccgagtgtga gcgcagcggc
      181 ggagcgggcg tcgccggtca cgaggagacc ggactcggcc ggcgtgagcg gagtgaagtc
      241 gccttgcccg tcgcctttca gcaggacgcc gaggccgccg tcgaaacgcc cggtgttggg
      301 ctcgggggcg aagttgttgc cggcgcagta gagatcggtc gcgccgtcgc cgtccagatc
      361 gtgcgcaaca atcgtgttga tcggcgcgat ttgcgcgtaa cgcgggagcg gctcgaaggc
      421 gaaggtgccg tcggattgtt ggaggaaaat gccgctggca agctcggtcg cggtgagttt
      481 ctgcgcgctc gtgagtttct ccggactgaa gatgtcgccg atcgtggctt gggcgtaggc
      541 tttgaacgtc gggaattttt tcgtgagcca cgggtaggag taggagagtt tgctgcgtcc
      601 acgaactgga tacagcttgc cgttctcgta gtgcgcttcg atgagctgcg atttgcccga
      661 gccgtcgagg tcgccggcgt agagcacggc gggttcctcg gcggaggcgt gatacttggt
      721 gttcaggccg acgttgccgg cgacgagatc ggggcggccg tcggcgttga cgtcggcgac
      781 ggtgagcgcg ctccaccaac cggtgtgggc ggcaaggccg gatttttccg tgacgttttc
      841 gaaggttttg ccggtgttgc gcaggtaggt gatcgcgccc cattcgaggg cgagcacgag
      901 atcgggcttt tggtcgccgt ccaagtcggc gaaggaggcg gcggtgacca tgccgatttc
      961 acggaggccg ggagcaagct cgtcggtgac gtcgacgaat tttccgccgg tgttgcgata
     1021 gaggaagctg cgcggggttt cgggatattt gccgggaacg acacggccgc cgacgaagag
     1081 gtcggttttg ccgtcgctgt cgaaatcggc gacggcgatc gaggcggtgc tgtggccgtc
     1141 ggcgggaagc gcgccgtcgg tggtggcgaa gttgccgcgg ccatcgttga ggtagagacg
     1201 gtcgttgaga agcgggtcgc cgacttcgcg ctcgatgccg ccggcggcga tcaggaggtc
     1261 gggatgaccg tcgttgttga cgtcgaagaa aacggcgccg gtgtcgtcg
//
LOCUS       DASPLT010000436         1270 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_148529, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000436 DASPLT010000000
VERSION     DASPLT010000436.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1270)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1270)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1270
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_148529"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1270)
                     /locus_tag="VEA63_04540"
     CDS             complement(<1..>1270)
                     /locus_tag="VEA63_04540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009510727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HYD83289.1"
                     /translation="AVAVGAMFIGAWGEAVLLLFLFSASGAMEEYALDRTQREVSALL
                     KSAPKTATLVLADGSEKEVAVGELQVGQRVLVRPGEAFSADGSVVKGRSASDESALTG
                     EAVPVEKGVGDQVFSGTINLWGAVEFEVARLPSESTLQKIIRLIQTAQKLKAPSERFT
                     DKFGTGYTYLVIGGSFAMFLVWWLGWGLPAFANTEETRSAFYRAMTLMVVASPCALVL
                     SIPSAILAAIAWGARHGVLFRGGAAIEKLAEVNAVALDKTGTLTTGELAVVGVESFPS
                     GREREVLELAYALEAKSQHPIARAIVRHAKAEGARVLELEEFQSITGQGLKGKLAGAN
                     LLLGRRELLESGPLAGWAARLPAAAAELSEVWVIGADLVGRVLLKDQIRAESRGVLSE
                     LKRMGIRTIMLTGDRRHAAEAVAKEIGLDEV"
BASE COUNT          225 a          534 c          293 g          218 t
ORIGIN      
        1 cacttcatcc aaaccgatct ccttcgcgac cgcttccgcc gcatgacgcc ggtcgcccgt
       61 cagcatgatc gtgcgaattc ccattcgctt cagctcgctc aacaccccgc gcgattccgc
      121 gcgaatctga tctttcaata acacccgccc caccaggtcc gcgccgatca cccacacttc
      181 gctcaactcc gccgccgccg ccggcagtcg cgccgcccag cctgccagcg gcccgctctc
      241 cagcaactcg cgccgcccga gcagcaaatt tgcccccgcc agtttccctt tcaacccctg
      301 tccggtgatc gattgaaact cctccagctc cagcacgcgc gcgccttccg ccttcgcgtg
      361 ccgcacgatc gcccgcgcga tcggatgctg cgatttcgcc tcgagcgcat acgcgagctc
      421 gagcacctcg cgctcccgcc cgcttggaaa actttccacg ccgacgaccg cgagctcacc
      481 cgtcgtcagc gtccccgttt tgtccaacgc caccgcgttc acttccgcga gtttctcgat
      541 cgccgcgccg ccgcgaaaca acacgccatg ccgcgcaccc cacgcgatcg ccgccagaat
      601 cgccgacgga atcgacagca ccaatgcgca cgggctcgcg acgaccatca acgtcatcgc
      661 ccgataaaac gccgaccgcg tctcctccgt gttcgcaaac gccggcaggc cccaacccag
      721 ccaccacacc aaaaacatcg cgaacgatcc gccgatcacg aggtaagtgt aacctgtgcc
      781 aaacttgtcc gtgaaccgct cgctcggcgc cttcaacttc tgcgccgttt ggatcagccg
      841 gatgatcttt tgcaacgtgc tctcggacgg cagtcgcgcg acttcgaatt ccaccgcgcc
      901 ccacagattg atcgtcccgc tgaacacctg atcgcccacg cctttctcca ccggcaccgc
      961 ctcgcccgtc agcgccgatt catcgcttgc gcttcgccct ttcaccaccg agccgtccgc
     1021 tgaaaacgcc tcgcccggcc gcaccagcac gcgctgcccg acctgcagtt cgcccaccgc
     1081 cacttccttt tcgctgccat ccgccagcac gagcgtcgcc gttttcggcg cgctcttgag
     1141 caacgcgctc acttcgcgct gcgttcgatc gagcgcgtat tcctccattg cgcccgacgc
     1201 tgagaacaag aacagcaaca acaccgcctc gccccacgcg ccgatgaaca tcgctccgac
     1261 cgccaccgcg
//
LOCUS       DASPLT010000437         1107 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_106504, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000437 DASPLT010000000
VERSION     DASPLT010000437.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1107)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1107)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1107
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_106504"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            163..>1107
                     /locus_tag="VEA63_04545"
     CDS             163..>1107
                     /locus_tag="VEA63_04545"
                     /inference="COORDINATES: protein motif:HMM:TIGR03790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03790 family protein"
                     /protein_id="HYD83290.1"
                     /translation="MTLVFVFGVWGRGAEELAARLVLLANGDDRDSVRVAQHYAAARG
                     VPEENIIALPMSRAETISWTEFVATIWEPLQAELVKRKWIDAIAMNLTDEVGRTKYAM
                     SGHRLSFLVVCRGVPLRVMHDPEHYVATPPYTNNAQFRTNAGAVDSELALLARPNYAV
                     NAFVPNPLFSDDKPTTTETAQVIKVSRLDGPTLKSAMGLVDSAMTAERTGLLGRAYID
                     IGGQHASGDRWLDAVARMLADLHLDMDVNRARSTIPATARFDAPVLYFGWYDGRVSGP
                     FTLPGFRFPPGAIAMHIHSYSAQTLRSAGQNWVGPFVAR"
BASE COUNT          177 a          289 c          424 g          217 t
ORIGIN      
        1 atgggcggcc gcggtgacga acgtgttggt gtcggtggcg ctgtgtttga tggcaacgtg
       61 gctggggtgg aggttggcgc gatgagggag gtgcgtgagg tgaaatcgcc tgcaagcagg
      121 ctcctgcctc cgatcaccgg aacgctccgt atctgcctcg gtgtgacgtt ggttttcgtt
      181 ttcggcgtct gggggcgagg agcggaggag ttggccgcgc ggctggtgct gctggcgaac
      241 ggtgacgacc gcgattcggt gcgggtcgcg cagcactatg cggcggcgcg cggcgtgccc
      301 gaagagaata tcatcgcgct gccgatgtcg cgggcggaga cgatttcgtg gacggagttc
      361 gtggcgacga tttgggagcc gttgcaggcg gaactggtga agcgcaagtg gatcgacgcg
      421 atcgcgatga acctcaccga cgaggtgggg cggacgaaat acgcgatgtc gggacatcgg
      481 ctttcgtttc tcgtggtgtg ccgcggcgtg cctttgcggg tgatgcacga tccggagcat
      541 tacgtcgcga cgccgcctta tacgaacaat gcgcaatttc gcacgaacgc aggcgcggtg
      601 gactccgagt tggcgctgct cgcgcggccg aactacgcgg tgaacgcgtt tgtgccgaat
      661 ccgcttttta gcgacgacaa gccgacgacg acggaaacgg cgcaagtgat caaggtctcg
      721 cgcctcgatg ggccgacgct gaaaagcgcg atggggctgg tggattcggc gatgacggcg
      781 gagcgcacgg gtctgctggg gcgcgcatat atcgacatcg gcgggcaaca cgcgagcggc
      841 gatcgctggc tggacgcggt agcgcggatg ttggcggatc tccatttaga catggacgtg
      901 aaccgcgcgc gttcgacgat tccggcgacg gcgcgcttcg atgcgccggt gctttacttc
      961 ggctggtatg acgggcgcgt gagcggaccg ttcacattgc cgggatttcg gtttccgccg
     1021 ggtgcgattg cgatgcacat tcacagttac tcggcgcaga cgctgcgttc cgcggggcag
     1081 aattgggtgg ggccgttcgt tgcgcgg
//
LOCUS       DASPLT010000438         1220 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_66181, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000438 DASPLT010000000
VERSION     DASPLT010000438.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1220)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1220)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1220
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_66181"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            266..>1220
                     /locus_tag="VEA63_04550"
     CDS             266..>1220
                     /locus_tag="VEA63_04550"
                     /inference="COORDINATES: protein motif:HMM:NF012200.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor D domain-containing protein"
                     /protein_id="HYD83291.1"
                     /translation="MKSSLVSAPRGRPGSCLLAGLLFLALPAVSVLAQNYTQNFGTAV
                     TPAPTGWTIVGGTWNVSGGTFNHTSNQFREKAIYSTTNWTSNYTYKARVWVTSGGSSG
                     NIAGIIFNYQGPRAYYDVTINNIGDVILSTINSPSADDVATPIASGTYSPSAPNTPVD
                     LEVIRGAGVTTVKVNGVVVINNVAQAALSGGGKIGVFASWATSKYDNVDVAAGAPEIE
                     VEGNSVVVADGDTTPSTTDYTDFGSAGVTGATVTKTYTIRNTGTAALTVSTVTAGGDF
                     AITAQPPSSIAAGGSGTFSVRFDPSATGVRTAAVSFINGDAN"
BASE COUNT          243 a          388 c          374 g          215 t
ORIGIN      
        1 caaacgcggc tgaacgaacg gccgatgccg cgccagcaac gcagcgaaca tttgtcaggc
       61 aaccggacgg cttcataggt cgcgcgcatg aaatgggggc atcacaaaac catcggtttt
      121 gatggcgaac atcggtggtc gaaaacgcgg ggtgcgtcaa taatggcgcc gcttcgtccg
      181 ccccgcgtcg ccttccggct cgtggtgcgg acgcggtggc ggcggcttcg tcgctggcct
      241 gctccaaaaa atgttaccct ccccaatgaa atcatctctt gtctccgctc cgcgcgggcg
      301 tccgggaagc tgtcttctcg ccggcctgct gttcctcgcg ttgccagctg tgtcggtgct
      361 cgcgcaaaac tatactcaga atttcggcac ggccgtgacg ccggcaccca ccggctggac
      421 gattgttgga gggacgtgga acgtctccgg cgggacgttc aatcacacgt ccaatcagtt
      481 ccgcgaaaag gcgatctatt cgaccacgaa ttggacgagc aattacacct acaaggcgcg
      541 cgtgtgggtc acgagcggcg gcagcagcgg caacatcgcc ggcatcattt tcaattatca
      601 aggcccgcgc gcctactacg atgttacgat caacaatatc ggcgacgtca ttctcagcac
      661 gatcaattcg ccgagcgcgg acgatgttgc cacccccatc gcatccggca cctattcgcc
      721 atcagcgccc aacacgccgg tggacttgga ggtgatccgt ggcgcgggcg tgacgaccgt
      781 taaagtgaac ggcgtggtgg tgatcaataa cgtcgcgcag gcagcgctca gcggcggcgg
      841 gaagatcggt gtgttcgctt cgtgggcgac ctcgaaatac gacaatgtcg atgtagccgc
      901 gggcgcgcca gaaatcgagg tggaggggaa ttccgtggtc gtcgccgacg gtgacacgac
      961 accctccacc accgactaca cggacttcgg cagcgccgga gtcacgggcg caacggtcac
     1021 taaaacctac accattcgca acaccggcac cgccgcgctc acggtcagca ctgtgacagc
     1081 aggcggcgat ttcgcgatca ccgcgcagcc gccttcctcg atcgcagcgg gcgggtcggg
     1141 cacgttcagc gtgcgtttcg atccgagcgc cacgggcgtg cgcaccgcgg ccgtcagctt
     1201 catcaacggc gatgcgaacg
//
LOCUS       DASPLT010000439         1780 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_148555, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000439 DASPLT010000000
VERSION     DASPLT010000439.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1780)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1780)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1780
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_148555"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..893
                     /gene="scpB"
                     /locus_tag="VEA63_04555"
     CDS             <1..893
                     /gene="scpB"
                     /locus_tag="VEA63_04555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007363064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HYD83292.1"
                     /translation="VTGAATGDGAAVSEGAAAAEGAASDAPGAGAEPVPAPTADLAAL
                     TGAQTGNPTVVTESAEIIVEPADDPELYADVPSLITATQIREVMDEIAAELRAADDGM
                     LLIEGGNGYRIVTHPRFARWVRILRSEPPPVKLSQSAIETLAIVAYRQPVTRGEIETI
                     RGVSAEAGINKLLERELVYVVGRADLPGRPIQYGTTDKFLEFVGVKSLDELPASDVLS
                     SRQIDEWLKTAMNPTSLSDTDMGLADEQLPLEGPATGEPVAEQIGGVDAATTQAEVSP
                     AEEAPEEKSETDDESNSAKN"
     gene            896..>1780
                     /gene="pheA"
                     /locus_tag="VEA63_04560"
     CDS             896..>1780
                     /gene="pheA"
                     /locus_tag="VEA63_04560"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HYD83293.1"
                     /translation="MDLQAIRAQIDQLDRQLVDVLNARLTLAAEIGKLKRSQGGQIYV
                     AEREDAVLRKVTGQNQGPIKNEALKAIYREIMSAAIALEKPLVIAYLGPEASNTHAAA
                     LKKFGASVDYHGMATVSDIFTAVEKGETDYAVIPIENSTEGSVREALDSFVESDLKIV
                     AQIYLEISHALISNSPLEDIERVYSKDQALAQCRHWLQRHLPHAQLIDAPSTSRAVQV
                     AKEEPGTAAIAGELAAEHYGVPVVERNIQDKADNTTRFFVIGRKPSGAVGGGKDLTSL
                     LVSLGDEAASHSGALLKML"
BASE COUNT          351 a          453 c          683 g          293 t
ORIGIN      
        1 ccgtaaccgg cgcggctacg ggagacggcg cggcggtgtc ggaaggcgcg gcagccgcgg
       61 agggtgcggc gtcggatgcg ccaggtgcgg gagcggagcc ggtgccggcg ccgacggcgg
      121 atttggcggc gttgaccggt gcgcagacag gaaatccgac ggtggtgacc gagtctgcgg
      181 aaatcattgt cgagccggcg gacgatccgg agctttatgc ggacgtgccg tcgttgatca
      241 cagcgacgca gattcgcgaa gtgatggacg agatcgcggc ggagttgcgc gccgcggacg
      301 atggcatgct gctgatcgaa ggcggaaacg gttatcggat cgtgacgcat ccgcggttcg
      361 cgcgctgggt gcggattttg cggtcggagc cgccgccggt gaaattgagc cagtccgcga
      421 tcgagacgct tgcgatcgtg gcgtatcgtc agccggtgac gcgcggcgag atcgagacga
      481 ttcgcggcgt gtcggcggag gcgggcatca ataaattgct ggagcgcgaa ctggtttacg
      541 tggtcgggcg cgcggatttg ccggggcgtc cgattcagta tgggacgacg gacaaatttc
      601 tggagttcgt cggtgtgaag tcgttggacg aattgccggc gtcggacgtc ttgtcgtcgc
      661 ggcagatcga cgagtggttg aagacggcga tgaacccgac gtcgctgagc gacaccgaca
      721 tgggtctggc ggatgagcag ttgccgttgg aaggccccgc gaccggcgag ccggtggcgg
      781 agcaaatcgg cggggtggac gcggcgacga ctcaggccga ggtgtcgccg gctgaagaag
      841 cgccggaaga aaaatcggaa acggacgacg agtcgaactc ggcgaaaaac tgaccatgga
      901 ccttcaagcg attcgcgcgc agatcgacca actcgaccgg caactcgtcg acgtgctgaa
      961 cgcacggctg acgttggcgg cggaaatcgg aaaactaaaa cgcagccagg gcggacagat
     1021 ttacgtcgcg gagcgcgagg atgcggtgtt gcgcaaggtg acgggccaga atcaggggcc
     1081 gatcaagaac gaggcgttga aggcgatcta tcgcgagatc atgtcggcgg ccatcgcgtt
     1141 ggagaagccg ctggtgatcg cgtatctcgg gccggaggcg agcaacacgc acgcggcggc
     1201 gttgaagaag ttcggcgcga gcgtggatta tcacgggatg gcgacggtga gcgacatctt
     1261 caccgcggta gagaagggcg agaccgatta cgcggtgatt ccgatcgaga actcgacgga
     1321 aggatcggtg cgcgaggcgt tggatagttt cgtggagagc gatctgaaga tcgtcgcgca
     1381 aatttatttg gagatcagcc acgcgttgat ttcgaactcg ccgctcgagg acatcgaacg
     1441 cgtttactcg aaggatcagg cgctggcgca gtgccggcac tggttgcagc ggcatctgcc
     1501 gcacgcacag ttgatcgatg cgccgagcac gtcgcgcgcg gtgcaggtgg cgaaggaaga
     1561 gcccggcacg gcggcgatcg ccggcgaact cgcggcggag cactacggcg tgcccgtggt
     1621 cgagcggaac attcaggaca aggcggacaa cacgacgcgg tttttcgtga ttggacgaaa
     1681 gccgtcgggc gcggtgggcg gcggaaagga tctgacgagc ttgttggtgt cgcttggcga
     1741 tgaagcggcg tcgcactcgg gggcgttgtt gaagatgctg
//
LOCUS       DASPLT010000440         1009 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_44971, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000440 DASPLT010000000
VERSION     DASPLT010000440.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1009)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1009)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1009
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_44971"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(233..>1009)
                     /locus_tag="VEA63_04565"
     CDS             complement(233..>1009)
                     /locus_tag="VEA63_04565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HYD83294.1"
                     /translation="DPTRPAVDEEALRTLGEALSLRSPPRTIECFDISHISGTFVVAS
                     MVHFANGRPDKNNYRRFQIKSFIGNDDFRAMEEVVGRRYRRLAEEGKLFPDLVVIDGG
                     RGQIGAALKAFVGLDAMPPPIIGLAKKHETIFFPDERAPLNLPLNHPGLNLLQRVRDE
                     AHRFANTYNAVLRSKKIRESVLEDFAGLGPVRRAALLAHFGSIDRLRAATVEEIAEVE
                     GFGPRMAEELHRFLNRPAMPESDAAPPEDAPEDSSNEKST"
BASE COUNT          189 a          296 c          339 g          185 t
ORIGIN      
        1 cgttgcagga tcgcgtgatc gctgaacagc ggagcgagcg tgatttcaga aaacccggcc
       61 gagggcagca acatggagaa caacagcagg cgaaaaatca tggggctgaa agacgattgt
      121 gagcgcgccg taattttccg agcgagaact gggttcgagg cgttcgcagc gaacacggcg
      181 cgacttacga agcacaacgc acgcgcttcg ccgcgcgcct ggcctgaagg gtctacgtcg
      241 atttttcgtt ggacgaatct tccggcgcgt cctcgggcgg ggcggcgtcg ctttcgggca
      301 tcgccggtcg gttgagaaag cggtgcagtt cctccgccat gcgcgggcca aaaccctcga
      361 cctccgcgat ctcctccacc gtcgccgcgc gcagacgatc gatgctgccg aaatgcgcga
      421 gcagcgcggc gcggcgcacg gggccgagtc ccgcgaaatc ctcgagcacg ctctcgcgaa
      481 ttttcttcga gcgcagcacg gcgttgtaag tgttggcgaa acgatgcgct tcgtctcgca
      541 cgcgttgcag caggttcagg ccgggatgat tcagcggcag attcagcggc gcgcgttcgt
      601 cggggaagaa gatcgtctcg tgttttttcg cgaggccgat gatcggcggc ggcatcgcgt
      661 cgagtccgac gaatgctttc aacgccgcgc cgatctgtcc gcgtccgccg tcgatcacga
      721 cgagatcggg aaacaatttt ccttcctcgg ccaaacggcg atagcgacgg ccgacgactt
      781 cctccatcgc gcgaaaatcg tcgttgccga tgaagctttt gatctgaaaa cggcggtagt
      841 tgtttttgtc gggccggcca ttcgcgaaat gcaccatcga ggcgacgacg aacgtgccgg
      901 agatgtgcga gatgtcgaag cactcgatcg tgcgcggcgg gctgcggagg gagagcgctt
      961 cgccgagcgt gcggagcgct tcctcgtcga cggcggggcg cgtgggatc
//
LOCUS       DASPLT010000441         1167 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_251141, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000441 DASPLT010000000
VERSION     DASPLT010000441.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1167)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1167)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1167
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_251141"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..460
                     /locus_tag="VEA63_04570"
     CDS             <1..460
                     /locus_tag="VEA63_04570"
                     /inference="COORDINATES: protein motif:HMM:NF036579.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-L-rhamnosidase C-terminal
                     domain-containing protein"
                     /protein_id="HYD83295.1"
                     /translation="ATTIWERWDSWTPEHGFNSEGMNSFNHYAYGSVIEWFYDTIAGL
                     KPDAATPGWKHFHIAPQPGGALTHAKATVETPYGPASSDWKIANNRFELTATIPPNTT
                     AHVSLPSADLSSATESNQPLSDLRAENLRVTENRIHLTLPAGTYRFSTPL"
     gene            complement(647..>1167)
                     /locus_tag="VEA63_04575"
     CDS             complement(647..>1167)
                     /locus_tag="VEA63_04575"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HYD83296.1"
                     /translation="AWVRVRGKAEENVAMVRDALRSLAVDEPPPRVAPLRDVAFRTLE
                     QLTRLAVFIGGLALALAAAGIHASVAFSTNQRTQEIGVRVALGATRASVLRLILRGGL
                     KVVAAGCALGVVAALVGLRLLFSLLSGSSGIDPVAIAGVMAFFTLIATAACLRPALKA
                     TKVDPMVALRAE"
BASE COUNT          258 a          489 c          251 g          169 t
ORIGIN      
        1 cgcgaccacg atctgggagc gctgggatag ctggacaccc gagcacggtt tcaactccga
       61 aggcatgaat tcatttaacc actacgccta cggctccgtg atcgaatggt tctacgacac
      121 catcgccggc ctgaaacccg acgccgccac tcccggctgg aaacatttcc acatcgctcc
      181 tcagcccggc ggcgctctca cgcacgccaa agccactgtc gaaacgccct acggccctgc
      241 gtcctccgat tggaaaatcg ccaacaaccg cttcgaactc accgccacga tcccgcccaa
      301 caccaccgcc cacgtcagcc ttccctccgc cgacctctca tccgccaccg agtccaacca
      361 accgctttcc gatctccgcg cggaaaacct ccgcgtcacc gaaaatcgaa tacacctcac
      421 cctccccgcc ggcacctacc gcttcagcac cccactgtag gaggggcttt acgccccgac
      481 tccccgcaac gccaaccgac cgatccaaac aaaacgctaa ccagcaacca cccatctccc
      541 ttccaccgca acaccccatc aacgagccaa cataacgccc agccccgtca tcgcccgcct
      601 ctctgctccc tccgcttcct cctgtaaaaa tagaaatagc ccgtcctcac tccgcccgca
      661 gcgccaccat cggatccacc ttcgtcgcct ttagagccgg ccggagacaa gccgcagtcg
      721 caatcagcgt gaagaacgcc atcacgcccg cgattgcgac gggatcgatc cccgagcttc
      781 cactcagcag agaaaacaac agccgcaatc cgacaagtgc cgccacgaca ccaagtgcac
      841 accccgcagc gaccaccttc aaccccccgc gcaaaatcaa acgtaatacc gatgcccgcg
      901 tcgctcccaa tgccacgcgc actccgatct cctgcgttcg ctggttcgtg gaaaaggcca
      961 ccgacgcgtg tatgcccgcc gccgcgagcg ccagcgccag cccgccgatg aacaccgcca
     1021 gccgcgtcaa ctgctcgagg gtgcgaaagg ccacatcccg taacggcgca acccgcggcg
     1081 gcggctcgtc cactgcgagg ctccgcagcg catcgcgcac catcgccacg ttctcctccg
     1141 cttttcctcg gacacgcacc catgccg
//
LOCUS       DASPLT010000442         1160 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_66216, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000442 DASPLT010000000
VERSION     DASPLT010000442.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1160)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1160)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1160
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_66216"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..505)
                     /locus_tag="VEA63_04580"
     CDS             complement(<1..505)
                     /locus_tag="VEA63_04580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD83297.1"
                     /translation="MTSGFAPGSLAEAQWQQAVRNNSRIAARAFAALFTAAMVTQYIL
                     LHTLADRRPGGYLLYSLPLLMGCMVIFEWRESGAASRTPTAPTAAKRVHSATLWVAAT
                     IEALAPISAIALLGSVFGWRVALGSPPLVAASLIPMVSILRLDPRVCVWQGVVAAMGF
                     GVMVLIAQ"
     gene            688..>1160
                     /locus_tag="VEA63_04585"
     CDS             688..>1160
                     /locus_tag="VEA63_04585"
                     /inference="COORDINATES: protein motif:HMM:TIGR04393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD83298.1"
                     /translation="MRNTLLGSALASALALASIAPPLHAAEVTDGAVPSTMDDAGTHY
                     IIGATGAHNRNLYTTIVGDLNAVTLTVQNGSILSSSSGLIGYGSTPEATGGSSVLVTG
                     PGSKWNMPNDLSVGGNTSGNSLTIANGGAVVTRGGSIGNGGSSANNAVTITGEGST"
BASE COUNT          224 a          427 c          329 g          180 t
ORIGIN      
        1 gttgcgcgat cagcaccatc actccgaaac ccatcgccgc cacgacgccc tgccacacac
       61 acacgcgcgg atcgagccgc agaatcgaca ccatcggaat caacgacgca gcgaccaacg
      121 gcggactccc cagcgccacg cgccagccga acaccgatcc gagcaacgcg atcgccgaga
      181 tcggcgccag cgcttcgatc gtcgccgcga cccagagcgt cgcagaatgc acgcgctttg
      241 ccgccgtcgg cgccgtcggc gttcgcgagg ccgctccgga ctcacgccat tcgaagatca
      301 ccatgcaccc catcagcaac ggcaggctgt agagcaaata gccgcccggc cgccggtccg
      361 cgagcgtgtg cagtaggata tactgcgtga ccatcgcagc cgtgaacagc gccgcaaacg
      421 cccgcgccgc aattcgcgag ttgttccgga cggcttgctg ccactgcgct tcggcgagcg
      481 accccggtgc aaacccgctt gtcatcccgc tcaccttgcg cgccgtcgct cgcgtcggca
      541 atcggagaac ctgtcatgcc gagcgctcag ccggacgtcg ggttccacct tcggccgctt
      601 cgctgccaac tgagtcaaat gactcaatca acgagttggc gcctccgccc gcagcccgct
      661 tcgaatcgcg ccttcacccc gatcaccatg cgaaacactc tcctcggttc ggcccttgcc
      721 agcgcgcttg cgctcgcctc catcgccccg cccctccacg ccgcggaggt cacggatggt
      781 gctgttccca gcaccatgga cgacgccggc actcactata ttatcggtgc cacgggcgcg
      841 cataaccgga atctctacac gacgatcgtg ggcgatttga acgcggtcac gctcacggtt
      901 caaaacggtt caatcctctc gagctcctca ggccttatcg gatatggatc taccccggag
      961 gcgaccggag ggagttcggt cctggtgacg ggcccaggct caaaatggaa catgccgaac
     1021 gatctcagcg ttggcggcaa tacctccggc aacagcttga cgatcgcgaa cggaggcgcg
     1081 gtcgttaccc gcggcggttc catcggcaac ggcggatcca gcgcgaacaa cgccgtgacg
     1141 atcaccggcg agggctcgac
//
LOCUS       DASPLT010000443         1498 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_190158, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000443 DASPLT010000000
VERSION     DASPLT010000443.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1498)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1498)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1498
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_190158"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..692)
                     /locus_tag="VEA63_04590"
     CDS             complement(<1..692)
                     /locus_tag="VEA63_04590"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HYD83299.1"
                     /translation="MAFHVSLIGRKNLSREICRQIAAAISDGRLKPGDRLSPTRELAR
                     ALAVSRMTVTVAYEQLAGEGFVRSQQGAGTFVTEAGARPSGRTAKRRGGGAVQPRAVW
                     DSLLEPVVLAPSLRYDFRTGLPDVSLFPHRAWHRAVSRALGSSETTMGIYEDAAGHRA
                     LRVAIARQIGFSRGVEAAADDIIITNGTQQALDLLSRVLLAPGDTIAVEDPSYGPPRL
                     LFNSLGLRVEAVP"
     gene            complement(785..979)
                     /locus_tag="VEA63_04595"
     CDS             complement(785..979)
                     /locus_tag="VEA63_04595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004111744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HYD83300.1"
                     /translation="MKNKLRELRARKEWSQGDLADKLGVSRQTINAIETEKYDPSLPL
                     AFKIAQLFKQPIEAIFESAD"
     gene            complement(976..1344)
                     /locus_tag="VEA63_04600"
     CDS             complement(976..1344)
                     /locus_tag="VEA63_04600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD83301.1"
                     /translation="MNAWLLVASVVYAVQRILLSRYPEWSPVARALVALSPLIPTLLY
                     LRSWVRFVRGMDELQQRVQLEAHVFAAWGAILLGVVLSVLHQHGVLDVLPHGLGFGGA
                     MVILFSLWLVGIRIADRRYK"
BASE COUNT          330 a          489 c          438 g          241 t
ORIGIN      
        1 ggaaccgcct ccacccgaag accgagcgag ttgaacagga gacgcggcgg cccgtaactc
       61 ggatcctcga ccgcaatcgt gtcacccggc gccagcaaga ctcggctcag gaggtcgagc
      121 gcctgttgcg ttccgttcgt aatgatgatg tcgtcggccg cagcctccac tccgcgcgaa
      181 aagccgatct gccgagcaat cgcgacgcga agcgcgcgat gacccgcggc atcctcgtag
      241 atgcccatcg tggtttcgct ggagcccagc gcgcgcgaca ccgcccggtg ccatgcgcga
      301 tggggaaaca gcgagacgtc gggcaaaccg gtgcgaaaat cgtagcgtaa tgacggcgcc
      361 aatacaacgg gctccaagag cgaatcccag acggcgcgtg gctggacggc accgccaccg
      421 cgccgttttg cggttcgacc agacgggcgc gcgcccgctt cagtgacaaa ggtgccggcg
      481 ccctgctgcg aacgcacgaa accttcgccg gccagttgtt cataggcgac cgtcacggtc
      541 atgcgcgaca ccgcgagcgc ccgcgcgagt tctcgggttg gcgaaagccg gtcgccgggc
      601 ttgagccggc catcggagat ggccgcggcg atctgtcggc agatctcacg actgagattc
      661 ttgcgaccga tcaagctgac gtgaaaagcc atcgcgttcc gcataggtgc cactgaagtg
      721 ggtcggctca cttacgcaca aaccgccgtg cagccacacc taatttgtcg tccaccgttc
      781 gcgctcagtc cgccgactcg aaaattgcct cgatcggctg cttgaacagc tgcgcaatct
      841 tgaacgcgag cgggaggcta ggatcgtatt tttccgtttc gatcgcgttg atcgtctgcc
      901 gcgagacacc gagcttgtct gccagatcgc cttgcgacca ttccttccgc gcgcgcaact
      961 cccgcagttt gttcttcatt tgtaacggcg atcggcgatt cggatgccga cgagccaaag
     1021 cgaaaacagg atcaccatcg cgccgccgaa gcccaacccg tgcggcagga catcaagcac
     1081 gccgtgctgg tgcagcacac taagaacgac gccgagaagg attgcgcccc acgcggcaaa
     1141 cacgtgggcc tcgagctgaa cacgctgctg caattcatcc atcccgcgca caaaccgcac
     1201 ccagctgcgc aagtagagca gcgtgggaat cagcgggctc aaagcgacca gcgcgcgagc
     1261 gacgggcgac cattccggat accgcgacag cagaatgcgc tgcaccgcat aaaccacgga
     1321 cgccaccaac agccaggcat tcaaacgcag atcctggcga aaagaatacc gccccgaaca
     1381 agggatgaca gattccaaaa ggttgggctt ttccatgcga agatcgagta aagcatactt
     1441 gtcacgtgtc aagcttgctt tactccagca aatccggcac cggcatcacc atcggaac
//
LOCUS       DASPLT010000444         2360 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_148579, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000444 DASPLT010000000
VERSION     DASPLT010000444.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 2360)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2360)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2360
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_148579"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(139..1416)
                     /locus_tag="VEA63_04605"
     CDS             complement(139..1416)
                     /locus_tag="VEA63_04605"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HYD83302.1"
                     /translation="MNRSAVRILIGVGLGIAAGFGAFVWVTGGSEKASAMGERSDANA
                     PVLNYESSPIGDPIAEHPVIAHVTIVDLDEDSLPDIVVCDALSNSVRWIRQFPRDTFT
                     ERQIGSTVQAPVHTTLADLNGDGRLDVLVASMGVLLPNNDRIGSVVVLENLGGGRFQN
                     RVIQDNLTRVTDVRAADINGDRRPDLIVGQFGYFEGHVQWMENLGDWQFKNHLLLTAP
                     GTIHTPVADFDGDGLPDFAALVAQDAEEVRIFRNRGDAAFEHTVVWKSLNPSWASSGL
                     EVGDVNRDGRSDLIFSNGDGFEGFVGVVPWHGLHWLENRENGFRAHSIGSLPGCYSPA
                     LADLDGDGDQDILAVSGFNNWRDPQAVSMMAWLNDGHEAFQPVVLAHTPTHLIAVAAG
                     DLDGDGTPELVTGGFHVYPPWQNMSRVTLWKRR"
     gene            complement(1433..>2360)
                     /locus_tag="VEA63_04610"
     CDS             complement(1433..>2360)
                     /locus_tag="VEA63_04610"
                     /inference="COORDINATES: protein motif:HMM:NF019229.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CRTAC1 family protein"
                     /protein_id="HYD83303.1"
                     /translation="LNTRYRASAAEPIVLFSGVFDGAGRKQLIECETIDGRLMPIRAR
                     DSLLAAMPGLRNRVPTYRAYARASIEEIVGPNPLANADRRSVTELRSGIFLSSPDSGR
                     PFRFVPLPRPAQLAPIFGLAAGDFDGDGRADLYAVQNSHAPHPEIGRFSGGISQLLRG
                     DGQGGLACVSPQESGLVVSGEARALAIKDFDRNGWPDFIVTRQNAAVLAFLNTPIEGR
                     NGFSVRLVGEGANDSAIGARITIEHASGARQVAEIQVGGGYLSQSSTARFFGFLDDDP
                     PREIRVQWPDGTSSAHSFPHGDTSIRLVKPVR"
BASE COUNT          480 a          733 c          704 g          443 t
ORIGIN      
        1 atcggccgag gtcgggcatc gcggacaggc cggcgaccgc gggattcggg ttaccgagga
       61 aactccatgc ggtcagaccc gccccggccg caaggagagc gaaaacgccg ctcaccatgt
      121 atccagtttt tctcgacatc atcgccgttt ccaaagggtc acccggctca tgttttgcca
      181 aggtggatac acatgaaaac cgcccgtcac gagttcgggt gtgccgtcgc catcgagatc
      241 gcccgccgcg acggcgatca gatgggtcgg cgtgtgtgcg agcaccaccg gctgaaacgc
      301 ctcgtggcca tcattgagcc acgccatcat cgagaccgct tgtggatccc gccagttgtt
      361 gaatccggag acagccagga tgtcctggtc gccgtcgcca tcgagatcgg ccaacgccgg
      421 gctgtagcag ccgggaaggg agccgatgga gtgggcgcga aagccgttct cccgattctc
      481 cagccaatgc agtccatgcc acggcacgac gcccacgaat ccttcgaagc cgtcgccgtt
      541 ggagaagatc agatcggacc ggccgtcgcg gttcacgtcg ccgacttcca aaccgctgct
      601 cgcccatgac gggttgagcg atttccagac aaccgtgtgc tcgaatgctg cgtcgccgcg
      661 attgcgaaat atccgaactt cttcggcatc ctgcgccacg agtgcggcaa aatccggcaa
      721 cccatcgccg tcgaaatccg cgactggcgt gtgaatcgtg ccgggagccg tcaaaagcag
      781 gtggttctta aactgccagt cgccgagatt ctccatccac tggacgtgac cttcgaaata
      841 accgaattgc ccgacgatca gatctgggcg tctgtcgccg ttgatgtccg ccgcgcgcac
      901 gtccgtgacc cgcgtgagat tgtcctgaat tacgcggttc tgaaaacgac cgccgccgag
      961 attctcaagc accacgacgc tgccgatccg gtcgttattc ggcaaaagca cgcccatgct
     1021 cgccaccaac acgtccaacc gtccgtcgcc attcaggtcc gccagtgtgg tgtgaacggg
     1081 tgcttgcacg gtggagccga tttgacgttc agtgaacgtg tcgcgcggga actgccgaat
     1141 ccagcgaacc gaattcgaaa gcgcgtcgca gacgacgatg tccggtaaag aatcttcgtc
     1201 taaatcaacg atggtcacgt gtgcgatcac cggatgctct gcaatcggat cgccgatcgg
     1261 tgagctttca tagttgagca ctggtgcatt cgcgtcagat cgttcgccca ttgccgacgc
     1321 tttctctgaa ccgccggtca cccacacaaa cgcgccgaag ccggctgcta tgccgaggcc
     1381 aacaccgatc aaaattcgca ctgcggaacg gttcatccgg aaagtgggct gatcaacgaa
     1441 caggttttac gagccgaatg ctcgtgtcgc cgtgcgggaa ggagtgagcg ctgctggtgc
     1501 catccggcca ttgcacgcgg atttcccgcg gcggatcgtc atccaggaag ccgaagaaac
     1561 gcgcggtgct cgactgactc agataaccgc cgccgacctg aatctcggcg acttgccgcg
     1621 caccgctggc gtgctcaatc gtgattctgg caccgatggc gctgtcattc gctccttcgc
     1681 cgacaagtcg aacggaaaat ccatttcgtc cctcgatcgg tgtattcaga aaagccaaca
     1741 cagcggcgtt ttgtcgcgtc acgataaaat ccggccagcc gtttcgatcg aaatccttga
     1801 tcgcaagtgc gcgggcttct ccgctcacga ccaagccgct ttcctgcggc gaaacgcacg
     1861 cgagaccacc ttgtccgtcg ccacggagta gttggctgat tccgccggaa aagcgcccga
     1921 tctccgggtg cggagcatgc gagttttgca ccgcgtagag gtcggcgcga ccatcgccat
     1981 cgaaatcgcc cgctgccagg ccgaagatcg gtgcgagttg agcgggacga gggagcggca
     2041 caaatcggaa cgggcggccg ctgtcgggag aactcagaaa aattccgctg cggagttcgg
     2101 tgacggagcg ccggtccgcg ttcgcgagcg gattcggtcc gacgatctct tcgatcgaag
     2161 cgcgggcgta ggcgcgatac gtcggcacgc gatttctcag tcccggcatt gcggccagca
     2221 gcgaatcgcg ggcgcgaata ggcatcagcc ggccatcgat cgtctcgcat tcgatcaatt
     2281 gttttcggcc ggcgccgtcg aagactccgg agaataatac gatgggttcg gcggcgctcg
     2341 cacgatagcg cgtattcaat
//
LOCUS       DASPLT010000445         1282 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_106566, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000445 DASPLT010000000
VERSION     DASPLT010000445.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1282)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1282)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1282
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_106566"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..451
                     /locus_tag="VEA63_04615"
     CDS             <1..451
                     /locus_tag="VEA63_04615"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HYD83304.1"
                     /translation="TTKPGIVTSVVEECRITLDQRHLDAAALARMLNEAREAAGRFAE
                     EGKVRVTWERLWQIEPILFHEELIAACEAAIVETCGRAHRLPSGPLHDAAEMARAGVP
                     TVMLFVQSLHGISHNKIEDTKEEHLALCVAALDRLAKKTMNWIALRQ"
     gene            511..774
                     /locus_tag="VEA63_04620"
     CDS             511..774
                     /locus_tag="VEA63_04620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD83305.1"
                     /translation="MNPATLVPTQFAMQELLGLVALATMLVGMWLNWQRHRHIEQIEE
                     DVKNGKLTGEQAWRRSRFVHRRATVVVCLGMALVLAFLIGLRG"
     gene            821..>1282
                     /locus_tag="VEA63_04625"
     CDS             821..>1282
                     /locus_tag="VEA63_04625"
                     /inference="COORDINATES: protein motif:HMM:NF014471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HYD83306.1"
                     /translation="MNASHPSFGEALRFWAKLGLISFGGPAGQIAIMHAELVEKKKWI
                     GERRFLHALNFCMLLPGPEATQLAIYCGWLLHRTWGGIAAGVLFVLPAAVLLWGLSYV
                     YVIYGDVPWIAAIFHGLKPAVAAIVAAAVLRIGRRALRNALMWAIAAMAFVA"
BASE COUNT          216 a          321 c          490 g          255 t
ORIGIN      
        1 cacgacgaag ccggggatcg tgacgagtgt cgtcgaggag tgtcgaatca cgctcgacca
       61 gcgtcacctc gatgccgcgg cgctggcgcg gatgttgaac gaggcgcgcg aggcggcggg
      121 ccgttttgcg gaggaaggca aggtgcgcgt gacatgggag cgattgtggc agattgaacc
      181 gattctgttt cacgaggagc tgattgcagc gtgcgaggcg gcgatcgtgg agacgtgcgg
      241 gcgcgctcac cggctgccat cggggccgtt gcatgacgcg gcggagatgg cgcgcgcggg
      301 cgtgccgacg gtgatgctgt tcgtgcagag tcttcacgga atcagtcaca acaaaatcga
      361 ggacacgaag gaggagcatc tcgcgttgtg cgtggcggcg ctcgatcgac tggcgaaaaa
      421 aacgatgaat tggatagcac tccgacaatg agcgctttta ctgcgccgtc ccatgttgct
      481 ggactacgct aaacaccctg ccggtttgcg gtgaaccccg ccacgcttgt gccgacccaa
      541 ttcgcgatgc aggaacttct cggactcgtg gcgctcgcga cgatgctggt cgggatgtgg
      601 ctgaactggc agcggcaccg gcacatcgag caaatcgagg aggacgtgaa gaacggaaag
      661 ctgacggggg agcaggcgtg gcggcgcagt cgttttgtgc accggcgggc gacggtggtt
      721 gtgtgtttgg ggatggcgct ggtgctcgcg tttctgatcg gactcagagg ctagccgcgg
      781 ggattgccgt cgcggagatg gatcacaagc gtcgcccgcc atgaacgcgt ctcatccgag
      841 ttttggcgag gcgctgcgtt tttgggcgaa gctgggcttg atcagttttg gcggaccggc
      901 ggggcagatc gcgatcatgc atgccgagct ggtcgaaaag aaaaaatgga tcggcgagcg
      961 gcgttttctg cacgcgctga atttctgcat gcttttgccg gggccggagg ccacgcagct
     1021 ggcgatctat tgcggttggt tgttgcaccg cacgtggggc gggattgcgg cgggcgtgtt
     1081 attcgtgctg ccggccgcgg tgctgctgtg ggggttgagc tacgtgtatg tgatttatgg
     1141 agacgtgccg tggatcgcgg cgatttttca cgggttgaag ccggcggtgg cggcgatcgt
     1201 cgcggcggcg gtgttgcgga tcggacggcg cgcgctgcga aacgcgctga tgtgggcgat
     1261 cgcggcgatg gcgttcgtgg cg
//
LOCUS       DASPLT010000446         1024 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_271337, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000446 DASPLT010000000
VERSION     DASPLT010000446.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1024)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1024)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1024
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_271337"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..383)
                     /locus_tag="VEA63_04630"
     CDS             complement(<1..383)
                     /locus_tag="VEA63_04630"
                     /inference="COORDINATES: protein motif:HMM:NF024240.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HYD83307.1"
                     /translation="MDTLNADVVVAGGGVGGCAAALALLENGHTVILTEEFAWIGGQF
                     TSQAVPPDEHGWIERFGCTRRYRRLREGIRDYYRQWYPLTPERRADPHLNPGNGWVSP
                     LCHEPRVALAVLESMLAPHVSAGRLR"
     gene            694..>1024
                     /locus_tag="VEA63_04635"
     CDS             694..>1024
                     /locus_tag="VEA63_04635"
                     /inference="COORDINATES: protein motif:HMM:NF012610.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HYD83308.1"
                     /translation="MRSSYQSISAQVAAVLRDEVGEGSWKQTLPSERRLAERLSVSRK
                     TVRKALAILRAEGWIRTEPNRASSIVSGAAKLRGGPVTTVALLLPEPLENARPFTVLW
                     VNRLMALL"
BASE COUNT          203 a          306 c          339 g          176 t
ORIGIN      
        1 cggagtcgac ccgccgacac atgcggcgcc agcatgcttt cgagcaccgc cagcgcgacg
       61 cgcggctcgt gacacagcgg cgagacccag ccattaccgg gattaaggtg cggatcggcg
      121 cggcgctccg gcgttagcgg ataccactgg cgatagtagt cgcggatgcc ttcgcgcagc
      181 cgccggtaac gccgggtgca tccgaagcgt tcgatccacc catgctcgtc gggaggaacg
      241 gcttgagacg tgaattggcc gccaatccag gcgaactctt cggtgaggat cacggtgtga
      301 ccgttttcca gcagcgcgag agcggcggca cagccaccca cgccaccgcc ggcgaccaca
      361 acatctgcgt tcaatgtatc catggattca attcgcgtgg gcgtcgctga ggagtcggag
      421 agggttcatg cagattgcgc gcgaacggag gactgaagcg actgcctcgg cgagcgaagg
      481 ccaaccaagg ggcgtcctca cggtcgaaca accctgtcaa cctggtgccc aaaggagacc
      541 gacggagttg gggtattttc taaaaaaatg acgcggcgaa ccgcgcgaca tgtcgccgaa
      601 ttgcggcaag caacgacacc cggtctctgg tggagaccac cttatacttg ctttcaggtg
      661 cgaggaaaga gattcactct tgctctcgct cccgtgcggt cctcttacca gtccatcagc
      721 gctcaagtcg cagccgtgct ccgcgatgaa gtcggggaag gcagttggaa gcagacgctt
      781 ccgagcgaac gccggttggc ggagcggttg agcgtcagtc gaaaaaccgt gcgcaaggcc
      841 ctggcgattt tgcgcgcgga aggctggatc agaacggagc cgaatcgcgc gagctcgatc
      901 gtgagcggcg cggccaaact tcgcggcggg ccggtgacga cggttgcgct gctgttgccg
      961 gaaccgttgg agaatgcgcg gcctttcacc gtgttgtggg tcaaccggct catggccttg
     1021 ctgc
//
LOCUS       DASPLT010000447         1263 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_66261, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000447 DASPLT010000000
VERSION     DASPLT010000447.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1263)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1263)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1263
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_66261"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..213)
                     /locus_tag="VEA63_04640"
     CDS             complement(<1..213)
                     /locus_tag="VEA63_04640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD83309.1"
                     /translation="MPEFEVTLERTITVRVFADDHADAAATAERAHEDFQAFAVEGTT
                     EGEPNGDLMEVVARCEACRKPIVAGLD"
     gene            complement(216..497)
                     /locus_tag="VEA63_04645"
     CDS             complement(216..497)
                     /locus_tag="VEA63_04645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD83310.1"
                     /translation="MRYLKAHNLLHRMPTMSTFRAKQIQNISAALTPDGPVTLEQNKA
                     TIQALLVMAATIGLATARIPFTSQSVEAISSRIERELRRQIRRIKGGTN"
     gene            complement(575..910)
                     /locus_tag="VEA63_04650"
     CDS             complement(575..910)
                     /locus_tag="VEA63_04650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD83311.1"
                     /translation="MGNYAIVIFGTGCHHNPDNTTDADKMAHQFVNKLAEAGHKIDAT
                     AFQVLTRGRAPEALREGVPVCDAFYRSEPPAEQAADSGNESSRSSNPPADDPAATNDE
                     AKQAEQASA"
BASE COUNT          218 a          385 c          373 g          287 t
ORIGIN      
        1 gtcgagcccg gcgacgatcg gcttgcgaca cgcctcgcag cgggcgacga cttccatcaa
       61 gtcgccgttg ggctcgcctt cggtcgttcc ctcgacggcg aacgcctgaa agtcttcgtg
      121 cgctcgctcg gcggtagcgg ccgcgtcggc gtgatcgtcg gcgaagactc ggaccgtgat
      181 cgtgcgttca agcgtgactt cgaattcggg catgattagt tcgtgcctcc cttgattcgg
      241 cgaatctggc gacgcagttc gcgctctatt cgacttgaaa tcgcctccac gctttggctg
      301 gtgaacggaa tgcgcgcagt ggccaatccg atcgttgccg ccatcaccaa gagcgcttgg
      361 atcgttgcct tgttctgttc caacgtcacc ggcccgtcgg gcgtaagggc ggcggaaatg
      421 ttttgaatct gttttgctcg gaacgtgctc atagtcggca tccggtgtaa aaggttgtgc
      481 gctttgaggt atcgcacgcg ctaagcaccg ccgtttttga acaggggtcg acggcactcg
      541 accgacggcg tcaaacgatc cctacagcaa tgggctacgc tgacgcttgc tcggcctgct
      601 tggcttcgtc gttcgtagcg gcgggatcat cggccggcgg attcgacgac cgcgacgatt
      661 cgttgccgga atcggcggct tgctcggcgg gcggttccga acgataaaac gcatcgcaca
      721 ccggcacgcc ttcccgcaac gcttcgggcg cacgcccccg cgtcaacact tggaacgccg
      781 tggcgtcaat cttgtgaccg gcctcggcga gcttgttgac gaactgatgc gccatcttgt
      841 ccgcgtcggt cgtgttgtcg gggttgtggt gacaaccggt tccgaagatg acgattgcgt
      901 agttacccat gattcaaact cctaatgatg aaaggcagaa agcgctcgtg ctctccgagc
      961 tgtcgcacca cttttcattt cgcaggtgtc gcgcggtttc gattaacgtg acttccactc
     1021 acgggcttcc ggttttacgt cgcccattcc aagctgcgac aacctgcatt tcagtctttc
     1081 gggtagacgg ggattgcttg acatttagcg accgtcttcc atccccaaag tcatccagtc
     1141 ggacgcacat ggttcgctac gccgcggtcg tcggatcaaa cacttccgag cgcagttccc
     1201 cggccgccgc ttggccggta atgagccaca ccttcgactc ggcgcacagt ttggcgagcc
     1261 aaa
//
LOCUS       DASPLT010000448         3148 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_251211, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000448 DASPLT010000000
VERSION     DASPLT010000448.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 3148)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3148)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3148
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_251211"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1001)
                     /locus_tag="VEA63_04655"
     CDS             complement(<1..1001)
                     /locus_tag="VEA63_04655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HYD83312.1"
                     /translation="MSSPNFGSGSLATLKPLLRSGVTSNSRSTNSRAIGNQDSANPWN
                     GASLARTAPIIALCHLSWDWVWQRPQQYLSRLAKTHRVLFVETYCSDVATTTVELRTP
                     DGHPNVTICAMHLPAKRWADGKFIDRERRRALQRTLVQELPGRFTDAILWFNDPMAVT
                     AFAGYLDESLIVYDCMDELSQFKGASPALLEREQDLLEVADVVFCGGRKMRDKRLRVN
                     PNCHFYGTGVDCRHFGSALSPATAVAPEIAALDGPVLGYFGVIDERIDYDLLAALADA
                     NPKWHVVMVGPFAKIDPTQLPQRRNLHWVGGRPYAQLPAFTKGFSVCLMPFALNEAT"
     gene            complement(1388..2662)
                     /locus_tag="VEA63_04660"
     CDS             complement(1388..2662)
                     /locus_tag="VEA63_04660"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HYD83313.1"
                     /translation="MSKVIISTLYDSDVFKMACRQFDKAADAINLPESIRDRTKYPRR
                     CLAVSLPIRRDDGSITVFEGYRVQHNLSTGPSKGGIRFHQNVTIGEVAALAMWMSWKC
                     SLVGLPYGGAKGGVIVDPRQLSPAELERLSRRYMQEMVNFLGPHVDVPAPDMGTNEQI
                     MGWMMDTYSNHVGHNEPAIVTGKPIALGGSQGRREATGAGVAYLAKCYLADLGIPLNE
                     STAVVQGFGNVGSEAALALQAYGLKVIALSDYTGGIYNSAGIDVRKAVAYLRSEGTLR
                     DFPEGDAITNEQLLELKCTVLAPCALERVITVDNAPRLQCRLLVEGANGPTTNGADRV
                     LDERGDIEIVPDILCNSGGVIVSYFEWLQNLQNYYWTRDEVMTKLTAILDRAKESVEY
                     QKRKFKFSRRLAALTLGIQRVADAKAARGLFP"
     gene            2778..>3148
                     /locus_tag="VEA63_04665"
     CDS             2778..>3148
                     /locus_tag="VEA63_04665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD83314.1"
                     /translation="MNDVAQRRLETKLAPSAVGRRSERIASKSAMAVVYHFVLRVARK
                     APLAIAAVAVLLIGAGAAVLFVDEQALKAGPRLRGELAAVEAKLAAVQDRIEQIQVDI
                     PPEQQRVLRSEKVIEQLKQLK"
BASE COUNT          599 a          824 c         1137 g          588 t
ORIGIN      
        1 tcggtcgctt cgttgagcgc gaacggcatc aggcaaacgg aaaagccttt cgtgaacgcg
       61 ggcagttggg cgtaagggcg gccgccgacc cagtgaagat tgcggcgctg cgggagctgc
      121 gtcggatcga tcttcgcgaa cgggccgacc atgacgacgt gccacttcgg gttggcgtcg
      181 gcgagggcgg cgagcaagtc gtagtcgatg cgttcgtcga tgacgccgaa gtagccgagg
      241 accggtccgt caagtgcggc gatctcggga gcgacggcgg tggcgggaga aagcgcactg
      301 ccgaaatggc ggcaatcgac gccggtgccg tagaagtggc agttgggatt aacgcgcagg
      361 cgtttgtcgc gcatcttgcg accgccgcag aaaacgacgt cggcaacctc gaggagatcc
      421 tgttcgcgtt cgagcagagc cggcgacgcg cctttgaact gggagagttc gtccatgcaa
      481 tcgtagacga tgagcgactc gtcgagataa cctgcgaaag cggtgacggc catggggtcg
      541 ttgaaccaca gaatcgcgtc ggtgaaacgg ccgggcagct cttgcacgag ggtgcgttgg
      601 agcgcgcgac gacgttcgcg atcgatgaat ttgccatcgg cccagcgttt ggccggaagg
      661 tgcatggcgc agatcgtgac gtttggatga ccgtcaggcg tgcgaagttc gacggtggtc
      721 gtggcgacat cgctgcaata cgtttcgacg aagagcacgc ggtgcgtttt cgcgaggcgc
      781 gagagatatt gttgcgggcg ttgccagacc cagtcccagc tcaaatggca gagtgcgatg
      841 atgggcgcgg tgcgtgcaag cgacgcaccg ttccacgggt tggcggagtc ctggtttccg
      901 atcgcgcgtg agttcgtcga cctggaattc gacgtaacgc cggagcgaag aagcggcttg
      961 agcgtggcga gggaaccaga accgaagtta ggagatgaca taggcgttga tgtgtttgaa
     1021 gagcatcgcg cggaattgtt ggaatagtag tccggcaagt gggggtttgg ttcgacgcgg
     1081 tcgaacgctc acgagcgcgg ctgcgcgtgg tgatgcttag ccgggcacgt gccacgaaaa
     1141 cgcgtgcgta agtcgcagcg catcaggcgg ggaatttccc gtcggagaaa ttgcggaaga
     1201 attctgcgtg caaattgccg gaacgctgtc gcgtgatcgg gcaaaatgac agacgtgtca
     1261 ggaaacgaac ggcgcgtgcg aggccgcgtc gttgaacgag cgcgcgcgtg aggaccgcga
     1321 tgaaggcgac gaaaagctca cgcttttcgc tacgcagaga ggtgttcgct acacgagggg
     1381 cgcgtgttta cggaaagagg ccgcgagcgg ctttggcgtc ggcgacgcgc tgaatgccga
     1441 gggtgagcgc ggcgaggcgc cggctgaact tgaatttacg cttctggtat tcgacggact
     1501 ctttcgcgcg gtcgaggatg gcagtgagct tcgtcatcac ctcgtcgcgg gtccaatagt
     1561 agttctgcag attctgcagc cattcgaaat aggagacgat cacgccaccg gaattgcaga
     1621 ggatgtcggg cacgatctcg atgtcgccgc gctcgtcgag aacgcggtcg gcgccgttgg
     1681 tcgtcgggcc gttggcgcct tcgacgagaa ggcggcattg aagacgagga gcgttgtcga
     1741 cggttatcac gcgttcgagc gcgcacggcg cgaggacggt gcacttgagc tcgagcagtt
     1801 gctcattcgt gatcgcgtcg ccttcgggaa agtcgcgcag cgtgccttcg gaacggaggt
     1861 aggcgacggc tttgcggaca tcgatgccgg cggaattgta gatgccgccg gtgtagtcgg
     1921 agagggcgat gactttgagg ccgtaggctt gaagcgcgag cgcggcttcg ctgccgacgt
     1981 tgccgaaacc ctggacgacc gcggtcgatt cgttgagcgg gatgccgagg tcggcgaggt
     2041 aacattttgc caaatacgcg acccccgcgc cggtggcttc gcggcggcct tgggagccgc
     2101 cgagggcgat gggtttgccg gtgacgatgg caggttcgtt gtggcccacg tgattcgagt
     2161 aggtgtccat catccagccc atgatttgtt cgttcgtgcc catgtcgggc gcgggcacgt
     2221 cgacatgcgg gccgaggaaa ttcaccatct cctgcatgta gcggcgcgag agtcgttcga
     2281 gctcggcggg agagagctgg cgcggatcga cgatgacgcc gccctttgcg ccgccgtagg
     2341 gcaggccgac gagcgagcat ttccaactca tccacatcgc gagcgcggca acctcgccaa
     2401 tcgtgacgtt ctgatggaag cgaatgccgc ccttcgaggg accggtggag agattgtgtt
     2461 gaacgcggta gccctcgaag actgtgatgc tgccgtcatc gcggcggatc ggcagcgaga
     2521 ccgcgagaca acggcgcgga tatttggtgc gatcgcgaat gctttcgggg aggttgatcg
     2581 cgtccgcggc tttgtcgaac tggcggcaag ccatcttgaa tacgtcggaa tcgtagagcg
     2641 ttgagatgat gactttcgac atggcgggag aagctcgatg cggagacgcg tgtgagtcgc
     2701 gcaggttcca tcggcgccgc gggggaacct tgcgacacaa ggcctctcta tggctcgctc
     2761 cgaccggagg attcgcgatg aacgatgttg cgcaaagacg tctggaaacc aagctcgcgc
     2821 cgagcgcggt cggacgtaga agtgagcgca tcgcctcgaa gtccgccatg gctgttgttt
     2881 atcacttcgt cctccgcgta gcccgcaagg caccgttggc gatcgcggcg gtggctgtgc
     2941 tgttgatcgg cgcgggtgct gcggtgcttt ttgtcgacga gcaagcgctc aaagccgggc
     3001 cgaggctccg cggcgagctg gctgcggtgg aagccaagtt ggctgcggtg caggatcgca
     3061 ttgagcagat ccaggtggac atcccgcccg agcagcaacg cgtgctgcga tcggagaaag
     3121 tgattgagca gctgaagcag ttgaagag
//
LOCUS       DASPLT010000449         1847 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_128056, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000449 DASPLT010000000
VERSION     DASPLT010000449.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1847)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1847)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1847
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_128056"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1826)
                     /locus_tag="VEA63_04670"
     CDS             complement(<1..1826)
                     /locus_tag="VEA63_04670"
                     /inference="COORDINATES: protein motif:HMM:NF012956.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitobiase/beta-hexosaminidase C-terminal
                     domain-containing protein"
                     /protein_id="HYD83315.1"
                     /translation="MNAFWSMSEVNVTNFKNNVSLVPNLAGIDARAENGAVIATAGNL
                     LLRTPDDANFADPANYDYRILPGSAAIDIGVSLAAADFPVTTDIEGNARPQGSAYDAG
                     ISEAGALSVFLIASPPPSVGATGSVNASTIGGTPPYTYAWSNGAGTASITGVPEGLYQ
                     VTVTDSLGAQKTRATYLIPGAVMGAPVSVTPANQVFAPTFNAPPGTYLTGQSVALVSA
                     TAGAAIRYTTDGTNPSDTVGTLYSTPIVVDNTVTLKAIAYKAGMVNSTVETATYIIDN
                     GPPNTQLTIAAVSESSHTSSNAPTNALDGNLGTSWASNGDGEWLQLDLGVPSRIGFLN
                     IAVGTATSRSYYYDLQASNDGLNWSTILTGGRTPLNAGLQKHDINDVQPVRYLRIVMH
                     GSNYSPTLNNIAEVEVWGGPAIGGGGEPPAAPTNLTAVSGDTVVTLEWDASTGANSYN
                     VKRATVTGGPYETVAAGVATTNFSDNNVMNTTTYYYVVTGVNLYGASDDSNEASATPQ
                     GLPPEPSESISAAPADGHVVVSWSPAAKTDAYTLKRATTAGGPYTTVAADLITTVFAD
                     VGLTNGTTYYYVVSGTNVDGEGANSTETSATPQAGYAGVLHIL"
BASE COUNT          314 a          557 c          618 g          358 t
ORIGIN      
        1 agaatgtgga gcacgccggc gtatccagct tgtggagtcg cactggtctc ggtgctgttc
       61 gcgccttcac cgtcgacgtt cgtgccggaa accacataat agtaggtcgt gccattcgtc
      121 aggccgacgt cagcgaacac cgtggtgatg agatcggccg caacggtcgt gtaaggtccg
      181 cccgcggtcg tggcccgttt gagcgtgtag gcatcggtct tcgcggccgg agaccaactc
      241 accaccacgt gaccatccgc cggcgcggcc gaaatgctct cggacggctc gggcggcagg
      301 ccttgcggcg tggcggatgc ctcgttcgag tcatcgctcg ctccgtagag attcactccg
      361 gtgacaacgt agtaatacgt cgtcgtattc ataacgttgt tgtcgctgaa gttcgtcgtc
      421 gcgacgccag cggccacggt ttcgtaagga ccgccggtga cggtggcgcg cttgacgtta
      481 tagctgttgg cgccggtcga cgcatcccat tcgagggtca ccaccgtgtc gccgctcacg
      541 gcggtaagat tcgtgggcgc cgcgggaggt tcgccgccgc cgccgatcgc cgggccgccc
      601 cagacttcga cttccgcgat gttgttgagc gtcggcgaat aattactgcc gtgcatcacg
      661 atgcgcaggt aacgcaccgg ctggacgtca ttgatgtcgt gcttctgcaa gcccgcgttc
      721 aaaggtgtgc gcccgccggt cagaatcgtc gaccagttga ggccgtcgtt cgacgcctga
      781 agatcgtagt agtaactgcg ggacgtcgcg gtgccgacgg cgatattcaa aaacccgata
      841 cggctcggca ctccgaggtc gagctgcagc cactcgccgt cgccgttgga cgcccacgag
      901 gtgccgaggt ttccatcgag cgcattcgtg ggggcgttcg aactcgtgtg cgaactctcg
      961 gagacggcgg cgatcgtcag ttgggtgttc ggcggaccgt tgtcgatgat gtaggtcgcg
     1021 gtttccacgg tgctgttcac catgcccgct ttgtaagcaa tggccttcag tgtgaccgtg
     1081 ttgtccacca cgatcggcgt cgaatagagc gtgccgacgg tgtcagacgg attcgtgcca
     1141 tcggtcgtgt aacggatcgc cgcgcccgca gtcgcggaga cgagcgccac gctctggccg
     1201 gtgagatacg tgccaggcgg cgcgttgaag gtcggcgcga acacctgatt cgccggcgtc
     1261 acgctcacgg gcgcgcccat caccgcgcca ggaatcaaat aagtggcgcg ggttttctgc
     1321 gcgcccaacg agtcggtgac cgtgacttga taaagcccct cgggcacgcc ggtgatcgat
     1381 gcggtgccgg cgccgttcga ccacgcgtaa gtgtaaggcg gcgtgccgcc gatcgtggac
     1441 gcgttaaccg aacccgtcgc gcccacgctc ggcggcggcg aggcgatcag aaaaacggag
     1501 agcgcaccgg cctcggaaat acccgcatcg taagcgctgc cctgcggacg cgcgttgccc
     1561 tcgatgtccg tcgtcaccgg gaaatcagcg gccgccagcg aaacgccgat gtcgatcgcg
     1621 gccgagccgg gaaggatgcg gtaatcgtag ttcgccggat cggcgaagtt ggcatcgtcg
     1681 ggcgtgcgca ggagaagatt tccggcggtg gcgatgaccg cgccgttttc ggcgcgcgcg
     1741 tcgatgcccg cgagattcgg cacgagcgac acattgtttt tgaagttggt gacgttgact
     1801 tcactcatcg accagaaggc gttcaccgcc ggattgatga tggtgtt
//
LOCUS       DASPLT010000450         1758 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_128073, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000450 DASPLT010000000
VERSION     DASPLT010000450.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1758)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1758)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1758
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_128073"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..946
                     /gene="yjfF"
                     /locus_tag="VEA63_04675"
     CDS             <1..946
                     /gene="yjfF"
                     /locus_tag="VEA63_04675"
                     /EC_number="7.5.2.9"
                     /inference="COORDINATES: protein motif:HMM:NF008630.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0103116 - ABC-type D-galactofuranose
                     transporter [Evidence IEA];
                     GO_process: GO:0042875 - D-galactonate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="galactofuranose ABC transporter, permease
                     protein YjfF"
                     /protein_id="HYD83316.1"
                     /translation="RLPQFATAAILAALYITACTLYEGFFSLPGAINLFTDNAALGIA
                     AVGMTLVIFSGGIDLSVGAVAGFTSIFVATLVKTHHVHPLLAIGAALVVGTALGAGMG
                     AIIHYFRQPAFLITLAGMFLMRGCAFWITTESVGITHPFYNTLSAFEITLGENFWLPA
                     TALCLLVMLVVGIVLAHYTRFGRNLLAIGGSEQSALLMGLPLAASRVSVYAFNSLCAT
                     CAGLVLVLYTGSGNPSFGLGLELDAIAVVVIGGTLLIGGRGHMLGTLLGLLIFGVIQA
                     ALIFDGRLSPAWSRIVVGALLLAFILLQRALLHRLSRR"
     gene            complement(1053..1427)
                     /locus_tag="VEA63_04680"
     CDS             complement(1053..1427)
                     /locus_tag="VEA63_04680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD83317.1"
                     /translation="MNKQVILLAFGLSLSAVSPVLAAGRNENKSHASARSEFEKPAPV
                     SVVKPVVSTRHIGERVNLVFHLDEQGRPNRITSTSRVAKDLVSTLVAAVSQWEFTPAR
                     DASGEPRTAKVLLPVRITEPSP"
BASE COUNT          265 a          681 c          454 g          358 t
ORIGIN      
        1 gcgcctccct caattcgcca ccgccgcgat cctcgccgcg ctctacatca ccgcgtgcac
       61 gctctacgag ggcttcttct cgcttcccgg cgccatcaat ctcttcaccg acaacgccgc
      121 tctcggcatc gccgccgtcg gcatgaccct cgtgattttt tccggcggca tcgatctctc
      181 cgtcggcgcc gtcgccggct tcacttcgat cttcgtcgcg acgctcgtca aaacccacca
      241 cgtccacccg cttctcgcga tcggcgccgc cctcgttgtc ggcaccgcgc tcggcgccgg
      301 catgggcgcc atcatccatt atttccgcca acccgcgttc ctcatcacgc tcgccggcat
      361 gttcctcatg cgcggttgcg ctttctggat cacgaccgaa tccgtcggca tcacgcaccc
      421 tttctacaac accctgtccg ctttcgaaat cacgctcggc gaaaacttct ggcttccggc
      481 caccgcgctc tgcctgctcg tcatgctcgt cgtcggcatc gtgctcgccc actacacacg
      541 cttcggccgc aacctcctcg cgatcggcgg cagcgaacaa tccgcgctcc tcatgggcct
      601 tcccctcgcc gcctcccgcg tcagcgtcta cgccttcaac agcctctgcg ccacctgcgc
      661 cggtctcgtt ctcgtccttt acaccggctc cggcaatccg agcttcggcc tcggcctcga
      721 actcgacgcc atcgccgtcg tggtcatcgg cggcaccttg ctgatcggcg gacgcggcca
      781 catgctcggc acgctcctgg gcctgctcat cttcggcgtc attcaagccg ccctcatttt
      841 cgacggacgc ctcagccctg cctggtcccg catcgtcgtc ggcgccctcc tcctggcctt
      901 catcctcctc caacgcgccc tgctccaccg cctctcgcgt cgttgatgta tccataaact
      961 gaagtcgcgc ggcccgccgc cctcacaaag aaaaagtggc tcgcgcctcg cagcggagcc
     1021 actcgtcttt ctcctcgtcg gtcaaccccg cttcaggggc tcggctcggt gatgcgaacc
     1081 ggcaaaagca ccttcgccgt cctcggctcg ccgctcgcat cacgcgcagg cgtgaactcc
     1141 cattgcgaaa ccgccgcaac cagtgtcgac acgagatcct tcgcgacgcg cgaggtgctg
     1201 gtgattcgat tcggacgccc ttgttcgtcg aggtggaaca ccagattgac tctctcaccg
     1261 atatgccggg tgctcacaac cggcttaacc accgacaccg gcgccggctt ctcgaattcg
     1321 ctacgcgcac tcgcgtgcga tttgttctcg ttgcgaccgg ccgcgaggac cggggagaca
     1381 gcgctcaggc taagcccaaa cgccaggagg atgacttgct tgttcattgt gcgactgagt
     1441 ccgccgtgtg acggactggt tttgttattt tgttttcttg ttggttggca tcgcggcacc
     1501 accgcccgtc gcatgcgcga aggcgtaggc caccccgacg tttccatccg cggcatcgaa
     1561 cgacaccgcg gacgcgaaag tctcatctca cctcgctgcg aacgcgccgc gtggtcgccc
     1621 ggagcgcaca cacgcatacc cgatcgcccg gatgtgccgg caatccgtgc cgcagcatcc
     1681 gcccagcacg acgagttgag gaaatttact gcgcaacgcc gcgtaatgcg ccgccagctc
     1741 ccacggatcg cccgcgtc
//
LOCUS       DASPLT010000451         1292 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_251248, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000451 DASPLT010000000
VERSION     DASPLT010000451.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1292)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1292)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1292
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_251248"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1209)
                     /locus_tag="VEA63_04685"
     CDS             complement(<1..1209)
                     /locus_tag="VEA63_04685"
                     /inference="COORDINATES: protein motif:HMM:NF012686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD83318.1"
                     /translation="MRFGSLHVLDLIVVLAYLVAVVWIGRRASKATQTEDGFFLAGRK
                     LGKLYQFFLNFGNATEPQGAVSTASFVYQQGAPGSWLSFQTVFMNPYFWFMNVWYRRV
                     RLTTLSDLFEDRYASRGLSLFYAIFQILVACVFLGFGNVTAYKIASSLVVKEAVEWTV
                     EDRASVEGYRELKRLEKLGTDAELAPEQATALANLRDRNARGELHSYITVLNDLSFYV
                     VFSIVVGTYIVLGGMAAAAVNEGLQSVLIIVFSLLLIPTGLAAIGGWSALSEKVPKQM
                     FDLFGSGSAEQFSVISLGAILLVSIVQNSGLSHNMGICGSAKNEMAARSGVSGTYLKR
                     LMIIMWAFAGLIAVAMFGPGGLSDPDATWGAMSNQLLGPGLVGLMLAGVLAGTMSTLA
                     AKALAISSLFV"
BASE COUNT          296 a          474 c          326 g          196 t
ORIGIN      
        1 cacgaacagc gacgaaatcg ccagcgcctt cgcggcgagc gtcgacatcg tgcccgccag
       61 cacacccgcg agcatcaatc ccacgagccc cggcccgagc aactggttcg acatcgcgcc
      121 ccacgtcgca tccggatccg acaacccgcc cggcccgaac atcgcgaccg cgatcaatcc
      181 cgcgaacgcc cacatgatga tcatgagccg cttcaaatac gtgcccgaca cgcccgagcg
      241 cgccgccatc tcgttcttcg ccgaaccgca gatgcccatg ttgtgactca atccgctgtt
      301 ctgcacgatg ctcaccagca aaatcgcccc gaggctgatc accgaaaatt gctccgcgct
      361 gccggacccg aacagatcga acatctgttt cggcactttc tcgctcaacg ccgaccaccc
      421 gccgatcgcc gccagccccg tcggtatcag cagcagcgaa aacacgatga tcaacacgct
      481 ctgcaatccc tcgttcaccg ccgccgccgc cattcccccg agcacgatgt aagtgccgac
      541 cacgatcgaa aacaccacgt agaacgagag atcgttcaac accgtgatgt aactgtgcag
      601 ctcaccgcgc gcatttcgat cgcgcagatt cgcgagtgcc gtcgcctgct ccggcgcgag
      661 ctccgcatcc gtgcccagct tctccagccg cttcaactcg cgatagccct cgaccgacgc
      721 gcgatcctcc accgtccact ccaccgcctc cttcaccacg agcgacgacg cgatcttgta
      781 agccgtcaca ttgccgaacc caagaaacac gcacgccacc aggatctgaa aaatcgcgta
      841 gaacaaactc agcccgcgac tcgcgtagcg gtcctcaaaa agatccgaca acgtcgtcag
      901 ccgcacgcgc cgataccaca cgttcataaa ccagaaatac gggttcatga aaaccgtctg
      961 aaacgacagc cacgaccccg gtgcgccttg ttgataaacg aaactcgccg tgctcaccgc
     1021 gccctgcggc tcggtcgcgt tgccgaagtt gaggaaaaat tggtagagct tcccgagctt
     1081 ccgtcccgcg agaaaaaacc cgtcctccgt ctgcgtcgcc ttcgacgccc gccgtccgat
     1141 ccacacgacg gcgacaagat aggcgagaac gacgatgaga tccagaacgt gcaggctgcc
     1201 gaaacgcatg aggtcgaaat ttgaacaacg atcgccgtgg agtggggaac tgcgggacgc
     1261 ccccgcggcg cgcggcgggc gtgcgaggga cg
//
LOCUS       DASPLT010000452         4813 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_106711, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000452 DASPLT010000000
VERSION     DASPLT010000452.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 4813)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4813)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4813
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_106711"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..633
                     /locus_tag="VEA63_04690"
     CDS             <1..633
                     /locus_tag="VEA63_04690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD83319.1"
                     /translation="GVGRVDFDFTGFAAATAGYSIIDLHLHRSCPDYLQMMDETLEWV
                     TRQGYRRVGLVLTYRPGGIGHKLFSSSFLYYQNQIREGDRVPILPKAQISDQALLRWI
                     DQYKPEAIISSGAVYQQLVRLRAPVPHKIKFSSIDLSESPHDAAGVDHGYRLVGHEVV
                     KLIHSQLLLNLSGVPEHPKVVLVDSHWRPGFTMPGNPSRREARATPLRQD"
     gene            769..3795
                     /locus_tag="VEA63_04695"
     CDS             769..3795
                     /locus_tag="VEA63_04695"
                     /inference="COORDINATES: protein motif:HMM:NF028442.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HYD83320.1"
                     /translation="MKRAPNLFQSCRRLLVCAALGLSSSLVACSLEKGFAEPPLSARP
                     LGWWHWINGNVTKEGIAADLQAAKNAGMGGVQLFDVEMYLPPGPVRYGTDNWYAHVRF
                     AMEKAAELGLEFHVMNTPGWSASGGPWVTPDRSMKRMVWTETSTDGGEIALQLPSPDL
                     KPIYSGRAVTAAPFYRDIAVFAVPATTERIPDFEKKAAWGARPITRPREPDRAGVARD
                     QVLNVTAKLDATGRLTVSLPPGKWTLLRMGFTTTGKTNHPAVKEGHGLEIDKLDAAAV
                     TFQFEQSLGRLLKEAGPLAGTTFNALLFDSFEGGFQNWTVSLPEAFQAQKGYDLMSWL
                     PVLTGRVIGSTAETEAVLWDFRNVIEELIAENYYGTMHRLAAKHGLKIYSESQGGPIT
                     PMSANRHVDVPMNEFWTPDAAGRASRIKMTTSSASFRGRNVVAAEAFTSTPENGKYQN
                     TPASLRRSGDYAFTLGLNRFALHSFTHQPVTELAPGFALGRYGVHFGRLNTWWPYADA
                     WMSYLSRSQFLLQQGRVVADVCLLVDEDLGYGLPAAIADSLPGYDYEVAYPDQVREMT
                     VRDGSLVHPSGLTFRVLVTPGKTTGKDWVAELSTLRYLRKLVESGAVLAGKPPVAPAG
                     WKDQQHRAEFDRLIVEIWGDAEEKETQVRQVGAGKVYADLPLLDLLRAENVEPDVSWG
                     ASDPSIKFIHRATPEADLYFVFNNSDQARRVALEFRQVGRTPEIWDPVSGARSDAPVF
                     DRTATGVAVPVDFEAWGSTFVVFRNPLSAHWVTSAEPVNMKFKNGGLTTTASEVTVNY
                     SDGHAKTLKFGTLPPSQSIDGPWQVEFKNARGAPPRVTFERLNSWTEHTDPAVRFYSG
                     TAVYRTEFFVGAVTHGQIAVLDLGEVADIARVFLNGREIGVLWQPPFRADVTSLLQEG
                     ANELEVHVANRWINRLIGDEAVDPGFRYQTSGGSKFTDGRLLTFPDWLYDPSKRASRP
                     RQSFSTWKHYDADSPLVPAGLLGPVKLEWLHPIALD"
     gene            4114..>4813
                     /locus_tag="VEA63_04700"
     CDS             4114..>4813
                     /locus_tag="VEA63_04700"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HYD83321.1"
                     /translation="MRVGSALLASFTLAVASHAQTPAESNGSLEGTVYNQASGLPISR
                     AKVVVRETGQEILTDDQGTFLFRQLPAKTLQLEVSYLGFVPQTIAVTVPPSGTTTQEF
                     RLSRERTSIRDEGEDETVVLDTFEVVAEQTMSGQAIAMNEQRHAANIKNVVAFDELGD
                     QGQENVGDYVRFLPGVAIVDDGENPGTIALGGFPAEMSNIQVDGVDVAGTGIGAQSSR
                     TVALQDVPIVNIERV"
BASE COUNT          932 a         1422 c         1507 g          952 t
ORIGIN      
        1 ggtgtcgggc gggtggattt cgatttcacc ggtttcgccg ccgcgacggc cgggtatagc
       61 atcatcgatc tgcatctgca ccgatcgtgt cccgactacc tgcagatgat ggacgaaacg
      121 ctcgagtggg tcacgcggca gggttaccgc cgggtgggac tcgtgctcac ctaccggccc
      181 ggcggcatcg gccataaact cttctcgtcc tcgtttctct attatcagaa tcagattcgt
      241 gagggagacc gcgttccgat tttgccgaag gcgcagatct ccgatcaagc gctcctccgc
      301 tggatcgacc aatataaacc ggaggcgatc atcagctcgg gcgcggttta ccaacaactc
      361 gtccgactcc gtgcgccggt tccccacaag atcaaattct ccagcatcga tctttccgag
      421 tctccgcacg atgccgccgg cgtggatcac ggctaccggc tcgtcggcca tgaagtcgtc
      481 aaactcatcc actcccagct tctgctgaat ctcagcggtg tgcccgaaca cccgaaggtg
      541 gtgctcgtcg acagtcactg gcggcccggc ttcacgatgc cgggcaaccc ctcgcgacgc
      601 gaagcgcgag cgacgccgct gcggcaggat tgagcgaaaa cgagagcttt tcgtcggccc
      661 ctcccgagat gcaaggaggt caacgcgtga ccatccgccg ggttgcagcg tcgccctcgc
      721 tcttcatgcg aacagacctc cgtcgcgaac gaaaatcctc agagcgtcat gaagcgtgcc
      781 ccgaatctgt ttcagagctg ccgccggttg ctggtttgcg cggcgctggg tctgtcgagt
      841 tcgctcgttg cgtgttcgct ggaaaaagga tttgccgagc cgccgctctc cgcgcggccc
      901 ctgggatggt ggcattggat caacggcaac gtgacgaaag aaggaatcgc cgccgatctg
      961 caggcggcga aaaacgcggg catgggcggc gtgcagctgt tcgacgtgga gatgtatttg
     1021 ccgccgggcc ccgtccgtta cgggacggat aactggtatg cgcatgttcg cttcgcgatg
     1081 gagaaagcag cggagctggg gctcgagttt cacgtgatga acacgcccgg ctggtcggcg
     1141 agcggcggcc cgtgggtcac gccggatcgc tcgatgaagc gcatggtgtg gacggaaact
     1201 tcgaccgacg gaggggaaat cgctcttcaa cttccgtcgc cggatctgaa gcccatctac
     1261 agcggacgcg ccgtgacggc ggcgcctttt tatcgcgaca tcgcggtctt cgccgtgccg
     1321 gcgacgacgg agcggattcc ggatttcgaa aagaaagccg cgtggggcgc gcgcccgatc
     1381 acgcgtccgc gtgagccgga tcgcgccggt gttgcgcgcg atcaggtttt gaacgtgacg
     1441 gcgaaactgg acgcgaccgg acgtctaacg gttagcttgc ctccgggcaa atggacactc
     1501 cttcgaatgg gtttcaccac gacgggaaag accaatcatc cggcggtgaa agagggacat
     1561 ggtttggaaa tcgacaaact ggatgcggcc gcggtgacat ttcagtttga gcaatcgctc
     1621 ggacgcctgc tgaaggaagc cggcccgctg gcgggcacca cgttcaacgc gctgttgttc
     1681 gacagcttcg agggaggatt tcaaaactgg accgtttcct tgccggaggc gtttcaggcg
     1741 cagaaaggct atgatttgat gtcctggctt ccggtgttga ccggccgcgt gatcggctcg
     1801 accgcggaaa cagaagcggt gctctgggat ttccggaacg taatcgaaga gctgatcgcc
     1861 gaaaattatt acggcacgat gcatcggctc gccgcgaagc atggattgaa aatctactcc
     1921 gaatcgcagg gcgggccaat cacgccgatg tcggcgaacc gccatgtgga tgttccgatg
     1981 aacgaattct ggacgcccga tgcagccggc cgcgcatcgc ggatcaaaat gactacgtcg
     2041 tccgcgagct ttcgcgggcg aaacgtggtg gcggccgagg cgttcacgtc gacgccggaa
     2101 aacggcaaat atcaaaacac gccggcgtcg ttgagacgct cgggcgatta cgcgttcacg
     2161 ctcggattga atcggtttgc gctgcacagc ttcacgcatc agccggtgac ggaactcgcg
     2221 ccaggcttcg cgctggggcg ttatggcgtg catttcggaa ggcttaacac ctggtggcct
     2281 tacgccgatg cttggatgag ctatctgagt cggagtcagt ttctgctgca gcaggggcgc
     2341 gtcgtcgccg acgtgtgttt gctggtcgac gaggacctcg gctacggctt gcccgcggcg
     2401 atcgcggatt cgctgcctgg ctacgattac gaggtggcgt atcccgatca ggtccgggaa
     2461 atgacagtgc gcgatggctc gctcgtgcat ccttcagggc tgacgtttcg cgtgctcgtc
     2521 acgcctggga aaacgacagg gaaagactgg gtggcggagc tgtcgacact gcgctatctc
     2581 aggaaactcg ttgagtcggg cgcggtgttg gcgggcaaac cgccggtggc gcccgcaggc
     2641 tggaaggacc agcagcatcg agcggagttc gatcgtctta tcgtggaaat ctggggagac
     2701 gccgaagaga aagagacgca ggtccgccaa gtcggcgcgg gcaaagttta tgccgacctc
     2761 cctctgctcg acctgctccg cgccgaaaac gtcgagcccg atgtgagctg gggcgcttcc
     2821 gatccgtcga taaaatttat tcaccgcgca acgcccgagg cggatctcta ttttgtcttc
     2881 aacaattcgg atcaggcccg gcgcgttgcc ctcgagtttc gtcaggtcgg tcgcacgccg
     2941 gagatttggg atccggtcag cggtgctcgc agcgacgcgc cggtgttcga ccgcacggcg
     3001 acgggcgtgg ctgtgccggt ggacttcgaa gcgtggggtt ccacgtttgt ggttttccgg
     3061 aacccgttgt cggcccattg ggtgacgtca gccgaaccgg tgaatatgaa atttaaaaac
     3121 ggcggtctca cgaccactgc atccgaggtg accgtcaact attccgatgg gcatgcgaaa
     3181 acgctgaagt tcggcacgtt gccgccgtcg caatcgatcg acggaccatg gcaggtggaa
     3241 ttcaaaaacg ctcgcggcgc gcctccgcgc gtgacgtttg agcgattgaa ttcctggacg
     3301 gagcacaccg atcctgcggt gcgtttctat tcaggcactg ccgtttatcg caccgaattc
     3361 tttgtcggcg ccgtcacgca cggtcagatc gcggtgctcg atctcggaga ggttgcggac
     3421 atcgcgcgcg ttttcctcaa cggccgcgag atcggcgttc tgtggcagcc gccgtttcgc
     3481 gccgacgtca cgtcacttct tcaagaaggc gccaacgagc tcgaggttca cgtggcgaat
     3541 cggtggatta accggctgat cggtgacgaa gcggtcgatc cgggatttcg ctaccaaacc
     3601 agcggcggaa gcaagttcac cgacggccgg cttctgacgt ttccggactg gctttacgat
     3661 ccgtcaaaac gcgcgagtcg tccgcgtcag agcttcagca cgtggaagca ctacgacgcc
     3721 gattcgcctt tggttcccgc aggattgcta ggcccggtca aactcgagtg gcttcaccca
     3781 atcgctttag actgatttca gaatcagccg gcgtgcgcct gctaactata taaaacaagt
     3841 cggctcgagg aaaaaatcag gtcaacggct taccgacgcg tttcttgccg cgcgctgggc
     3901 gggaacgcgt aatcgccacg acctctggag aagaacatat attcttttcc tcaccccgac
     3961 cctgaccccg cttacaccat gggtagttta cacgctctgc tatcttcaat tcgcctgccc
     4021 gcgtttatcg cggttacgcc cgcaagtctt ctgaacgctc gaacgagccg acgcgcgccc
     4081 gaagctgaga acggcctccg cacgctcggt ctgttgcgcg tcggctcggc gctcctcgca
     4141 agcttcacgc tggccgtcgc cagtcacgcg caaactccag ccgagtccaa cggctcgctc
     4201 gagggcaccg tgtataatca ggcgagcgga cttccgatca gccgcgccaa ggtggttgtt
     4261 cgcgaaacag gtcaggaaat tctgactgac gaccagggaa cttttctctt tcgccagctc
     4321 ccggcgaaga cgcttcagtt ggaagtgtcg tatctggggt ttgtgcccca aacaatcgcc
     4381 gtcaccgtgc cgccaagcgg gacaactacg caggagttcc gcctgagtcg cgagcgcacc
     4441 agcattcgcg acgaaggaga ggacgaaacc gtcgtgctcg atacatttga agtcgtggcc
     4501 gagcaaacga tgagcggtca agcgatcgcc atgaacgagc agcgccacgc agccaatatc
     4561 aagaatgtgg tcgcgttcga tgaactcggc gaccagggcc aggagaacgt tggcgattac
     4621 gtgcgttttc tccctggcgt cgcgatcgtg gacgatgggg agaatcccgg caccatcgct
     4681 ctcggaggtt ttcccgccga aatgtccaac atccaggtgg acggcgtcga cgtggccggc
     4741 acgggtattg gcgcccaaag cagtcgcacc gtggcgctgc aggacgtgcc gatcgtgaac
     4801 atcgagcgcg ttg
//
LOCUS       DASPLT010000453         1269 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_87499, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000453 DASPLT010000000
VERSION     DASPLT010000453.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1269)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1269)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1269
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_87499"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            379..>1269
                     /locus_tag="VEA63_04705"
     CDS             379..>1269
                     /locus_tag="VEA63_04705"
                     /inference="COORDINATES: protein motif:HMM:NF012742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HYD83322.1"
                     /translation="MNSDARPTMRSLAQALGLSRTTVSDSLRNHPRVKAETRARVQAH
                     AREIGYRFNPLASSIFSDVRRTRLSAFQGVLAAVSLEEPSRPQFTGPYWQDLLRGAAE
                     RADQMGFKLERFLVGAPGVSVHRLDTILQSRGIRGVLIMPAWGRPDFTRLDWKNYTGV
                     YSDYLIDRPALHSVCPDHLRAMVGALERLHALGYRRPGLVLQEQESVRLQHRWAAAFL
                     AHVNLCKGFKAAPLLVVPQLTKAPFTQWFKRHKPDVVLGHRSELVSWMEECGARVPQT
                     HGFCCLNISINATPCAGVDQQ"
BASE COUNT          212 a          412 c          405 g          240 t
ORIGIN      
        1 cggtgagagt tccaccacct cgtcatttgc ggggcgaggt gcgggagcag agtcggcttg
       61 ggcccgagcg gccgaagcgc cggcagcggc aagacttgcg aacagggtca agcgagctac
      121 gcgcgatagt ggggtgcggt gcagtttcat gcggaaggct tggttgcgga gggcgccagc
      181 cggtcacgaa ttttcgtcgg gggtattgtc cgtgagaata cgagacagtc tggcgggggt
      241 taagcggcgg ccgccgagct tggcggtcgc tacagacctg caccgtgacg ctctgcttca
      301 ccggatcgaa ccgccgcgat tcgtcccgtc agaattctgc gagctgggca ggcggacagt
      361 ccgtgtcaaa ctgcgcgcat gaacagcgac gcccgcccca ccatgcgcag cctcgcccaa
      421 gcgcttggcc tttcgcgcac caccgtttcg gattcattgc gcaatcatcc gcgcgtcaag
      481 gccgagaccc gcgcccgcgt gcaggctcat gcgcgcgaga tcggctatcg tttcaacccg
      541 ctcgcgagtt cgatctttag cgacgtgcgt cgcacacggc tgtcggcttt tcaaggtgtc
      601 ctcgccgccg tcagtctcga agagccgagc cgcccgcagt tcaccggccc gtattggcag
      661 gaccttctgc gcggagcggc cgaacgcgcc gaccagatgg gtttcaagct cgaacgtttt
      721 ctggtgggag cgcccggcgt ttccgttcac cggctcgaca cgattctgca atcgcgcggt
      781 atccgcggcg ttctgatcat gccagcgtgg ggccggccgg atttcacccg actcgactgg
      841 aaaaattaca cgggggttta ctcggactac ctcatcgatc ggcccgcgtt gcattccgtc
      901 tgccccgatc atttgcgcgc gatggtgggc gcgctcgagc ggctgcatgc gctcggctac
      961 cgtcgtccgg gactagtgct ccaggaacag gaaagcgttc gcctgcagca tcgctgggcc
     1021 gccgcgtttc ttgcgcacgt caacctctgc aagggtttca aggccgcgcc gctgctggtc
     1081 gttccgcaat taaccaaagc gccgttcacg cagtggttca aacgccacaa gcccgatgtc
     1141 gttctcggac accgcagcga actcgtctcg tggatggaag agtgcggcgc gcgcgtcccc
     1201 cagacccacg gcttctgctg cctgaacatc agcatcaacg ccacgccttg cgccggcgtc
     1261 gatcagcag
//
LOCUS       DASPLT010000454         1931 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_106832, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000454 DASPLT010000000
VERSION     DASPLT010000454.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1931)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1931)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1931
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_106832"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            171..704
                     /locus_tag="VEA63_04710"
     CDS             171..704
                     /locus_tag="VEA63_04710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HYD83323.1"
                     /translation="MSAETEEWFDVVNERDEPVARALRREVHARNLLHRAVHVLVFDR
                     HGRVFLQKRSMKKDMCPGLWDSSCSGHLDAGEDYDTAAQRELGEELGLTVVRPPERWF
                     RVEACEATGWEFCWIYRMESGGPFVLHPDEIDGDEWLSPTEVNTRIEAKPEEYCPAFK
                     LIWGIARAKLGVGVLNR"
     gene            complement(893..1210)
                     /locus_tag="VEA63_04715"
     CDS             complement(893..1210)
                     /locus_tag="VEA63_04715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD83324.1"
                     /translation="MSIEFGWWNKDPEQGKYQVSAVVHGGNIAWTRHQGHHTQWQPHT
                     PTDEDRARLVYEAERRLPRRLITQKQFEEIKRLSANAGPGAISGRHSSRPGPTSEYDL
                     SGP"
     gene            complement(1232..>1931)
                     /locus_tag="VEA63_04720"
     CDS             complement(1232..>1931)
                     /locus_tag="VEA63_04720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD83325.1"
                     /translation="LDTRPGHAPGFVAALRSDVDRFFHLAALPTTCLTGNHDPDFSAH
                     HHLELANGGVFLTHGDILFEDIVPWSQDARLARDLVAAELAKLSPAERHDVASRLSAY
                     RRAAFAIPQRHQSAPRGLQHLLGLAQETIWPPTRVFRILRAWREVPSRVDRFLREHRP
                     NARFFIMGHTHRLGALRTAGGIVVLNTGSFCPPGGGGVIDLTPDSLSLRRVERSGAGF
                     RLGRTLAEFSLARP"
BASE COUNT          364 a          495 c          735 g          337 t
ORIGIN      
        1 gacagtccgc gcgatgcgac gcgtgtgacg gtgaacgatc tcgcgaacgc gttggaagac
       61 catcttgcgg gccgcgcgat tcgcacgccg aagacgaacc cgatcggatg caatgtaaag
      121 tgggaaggga aagacgcgca ttggatgccg gcggaggcgt gcgatctggt gtgagcgcgg
      181 aaaccgaaga gtggttcgat gtagtgaacg agcgcgacga gccggtcgcg cgggcgctgc
      241 gtcgcgaggt gcatgcgcgc aacctgttgc accgcgcggt ccacgtgctg gtgttcgatc
      301 ggcacggacg cgttttcctg cagaaacgtt cgatgaaaaa agacatgtgt cccggcctgt
      361 gggactcgtc gtgttccggg catctcgatg ccggcgagga ctacgatacc gcggcgcagc
      421 gcgaactcgg tgaggagctt gggctcacgg ttgttaggcc gccggaacgg tggtttcgag
      481 tcgaggcgtg cgaggcgacg ggttgggaat tttgctggat ttaccgaatg gaatcgggcg
      541 gaccattcgt gctgcatccg gatgaaattg atggcgacga gtggctgtcg ccgacggaag
      601 taaacacgcg gatcgaggct aagccggaag agtattgtcc ggcgttcaaa ctgatctggg
      661 gcatcgcgcg cgcaaagttg ggcgtcggag ttttgaaccg ctaatcggcg ctaatttttc
      721 agaggcgggt tgtgagggcg gttgagtgcg tgcttcgcct gatcggcgca gcgcggacgc
      781 gggctcggaa ctcgccggcg ttttgccggg atgaagccag attgatggcg gcaaggaggc
      841 gcagaaggcg caaaatgtag ttcgcgaaaa cgcgcggtta ggccgtagcc ggttacggtc
      901 cgctgaggtc gtattcggac gtcgggccgg ggcgggagga gtggcggccg gagatggcgc
      961 cggggccggc gttggcgctg aggcgtttga tttcttcgaa ctgtttttga gtgatgagcc
     1021 ggcgggggag gcgacgttcg gcttcgtaaa ccaggcgagc gcgatcctcg tcggtgggtg
     1081 tgtgcggctg ccactgggtg tggtgccctt ggtgacgcgt ccaggcgata ttgccgccgt
     1141 ggacgacggc gctgacctga tacttgcctt gctcaggatc cttgttccac caaccgaact
     1201 cgatgctcat acgacggagt gtcgccggcg ctcaggggcg agcgagtgaa aattcagcga
     1261 gggttcggcc gaggcgaaat ccggcgccgc tgcgctcgac gcgccgcagg ctcaggctgt
     1321 cgggcgtgag gtcgatcacg ccgccaccgc cgggcgggca aaacgaaccg gtattgagaa
     1381 caacgatacc gccagcagtg cggagagcgc cgagacgatg ggtgtgcccc atgatgaaaa
     1441 aacgcgcgtt gggtcgatgt tcgcgcagaa agcggtcgac tcgcgaaggg acttcgcgcc
     1501 aggcgcggag aatgcgaaag acgcgcgtcg gcggccagat ggtctcttgg gccaaaccga
     1561 gcagatgttg gaggccgcgt ggcgcggatt ggtggcgctg cgggatcgcg aaggcggcgc
     1621 gacgataagc gctcaagcgc gaggcaacat cgtggcgttc ggcgggcgaa agctttgcga
     1681 gttccgcagc gaccaaatcg cgggcgagac gcgcgtcctg gctccaaggc acgatgtctt
     1741 cgaacaggat atcgccatgc gttaggaaga cgccgccatt cgcgagttcg agatgatggt
     1801 gagcggagaa gtcgggatcg tgattgcccg tgaggcaggt cgtcggaagc gcggcgagat
     1861 ggaaaaaccg gtcgacgtcg gagcggagtg cggcgacgaa gcccggggcg tggccggggc
     1921 gcgtatcgag c
//
LOCUS       DASPLT010000455         1445 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_45108, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000455 DASPLT010000000
VERSION     DASPLT010000455.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1445)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1445)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1445
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_45108"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(431..>1445)
                     /locus_tag="VEA63_04725"
     CDS             complement(431..>1445)
                     /locus_tag="VEA63_04725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD83326.1"
                     /translation="DAERIDDAVFQSAEAHTRDMNAILDTFVERTTVRDASFELPTSG
                     ELQPGSENGTPRPTQGYQTISQGYPMFRGMDSFGLNREAYAKLTVREMDKLMAGVQSK
                     DARWIMRRVFGAIFTNVSWTFKEPGRSDLTVRGLAVTGDGSIYLDFNGDLTTADHYTG
                     QADAISNSANPYEANSAILRTHPANTGVIVSYIPPGLMATTAALEGFYPYQPNDGLVD
                     FGGLVDVAAANVGAFQRFGNEVMGVVGDNVIVVSRRLPAGYVVSVVDTDEAPLVMREE
                     PEAELQGLQAVPMVVDSNFRKFDFYRKAGFAVRNPIAIAVREISDATYDIPSGYDLRT
                     LQG"
BASE COUNT          242 a          455 c          467 g          281 t
ORIGIN      
        1 gagcttattg agttccgccg gggtgaccgt cacgccgtcc agcacgttga gttcggcggc
       61 ggttgtcgtg acttcggtcc cgttaagata caggcgttcg gtggcgttga gttcgggaac
      121 gcgccgctgg gggcgtttcc acagggtgac tcccatcctc ggtcctttct tcctcgtccg
      181 catcgggcgg agggtccgtc gccgtgccgg ttacggtcac ggtggccgcg agggcatcca
      241 gccagtccgc cagcgccgca tagcgcacgc tttcgagata ggcggggtcg cggctatatt
      301 cgggcaattg aggcggcgcg atgccgaacg cgcgggcgat ctcccgcgtg gagatcgcca
      361 tgcgcttcag agccgattca cgccgcttgg ccgtcgatac aggatcgggc attgtgtcct
      421 ttgccttccc ttaaccttgc agcgtgcgca ggtcgtaacc gctcggaatg tcatacgtcg
      481 cgtcggaaat ttcgcgcacg gcgatagcta tagggttacg gacagcgaag cccgctttcc
      541 tataaaaatc gaacttccta aaattggaat cgacgaccat gggaaccgcc tgcaagcctt
      601 gcagttcggc ttccggttcc tcgcgcatga ccagcggcgc ttcgtcggta tcgaccaccg
      661 acaccacgta gcccgcaggc aggcggcgcg acacaacgat cacgttgtcg ccgacgacgc
      721 ccatcacttc gttgccgaag cgctggaacg cgccgacatt cgctgccgcc acgtccacca
      781 acccgccgaa atccaccagc ccgtcgttcg gctgatacgg gtagaaccct tccagtgcgg
      841 cggtggtcgc catgaggccg ggcgggatgt aggacacgat cacgccggta ttggcggggt
      901 gcgtgcgcag aatggccgaa ttcgcctcat aggggttggc gctgttggaa atggcgtcgg
      961 cctgcccggt gtagtggtcc gccgtcgtca ggtcgccgtt gaagtcgaga tagatgctgc
     1021 cgtcacccgt gacggcgagg ccgcgcacgg tgagatccga gcgccccggc tccttgaacg
     1081 tccacgagac gttggtaaag atcgcgccga acacgcgccg catgatccag cgcgcgtcct
     1141 tcgactggac gcccgccatg agcttgtcca tctcgcggac agtcagcttg gcatacgctt
     1201 cgcggttcag cccaaagctg tccataccgc gaaacatcgg gtagccctgc gagatcgtct
     1261 gatagccctg cgtcgggcgg ggcgtgccgt tctcgctgcc gggctgcaat tcgccggacg
     1321 tgggcagctc gaacgaggca tcgcggacgg tggtgcgctc aacaaaggtg tcgaggatcg
     1381 cgttcatgtc gcgggtgtgc gcttccgcac tctggaacac cgcgtcatcg atgcgttcgg
     1441 cgtcc
//
LOCUS       DASPLT010000456         1162 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_190544, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000456 DASPLT010000000
VERSION     DASPLT010000456.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1162)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1162)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1162
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_190544"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..312)
                     /locus_tag="VEA63_04730"
     CDS             complement(<1..312)
                     /locus_tag="VEA63_04730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD83327.1"
                     /translation="MTREELLQQVTAIRWFHQIPLGNGVVTPGIDDSVAKLRRVELPP
                     SLGGKTVLDVGAWDGFFSFEAEKRGASRVVAIDSYCWNGDGWGSKRGFELARRALGSK
                     VE"
     gene            complement(403..>1162)
                     /locus_tag="VEA63_04735"
     CDS             complement(403..>1162)
                     /locus_tag="VEA63_04735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AIR synthase-related protein"
                     /protein_id="HYD83328.1"
                     /translation="GDSGWFGDEKRTMALVDGFAEGCKAAGAVWGGGETPTLGGVVNP
                     EAIVLAGSAIGKISPKNLRITGEVKEGDKIIFLASSGVQTNGLSLCRRVAAHLPDGYQ
                     TPVGFGDPRTFGEALLAPSVIYVAFVRECQERGIKLNYVAHVTGHGWRKLMRLDEPFV
                     YEIEHVREPLAVFKFLEKMGPISQREMYATFNQGIGFAAYVSPENVDAVLAVAKATGY
                     DAWLAGTVKKEGARKAVVVRSLGITFEGDTLQVR"
BASE COUNT          222 a          468 c          274 g          198 t
ORIGIN      
        1 ctccaccttc gacccgagcg cacggcgcgc gagctcgaag ccgcgcttgc tgccccagcc
       61 gtctccattc caacaatacg agtcgatcgc cacgacacga ctcgctccgc gcttctccgc
      121 ctcgaacgag aaaaaaccgt cccacgcgcc cacatccagc accgtcttcc cgccgagcga
      181 cggcggcagc tccacgcgcc gcagcttcgc caccgaatcg tcaatcccgg gcgtcacgac
      241 gccattaccc agcggaatct ggtgaaacca ccggatcgcg gtcacctgct gcagcaactc
      301 ctcacgcgtc atcgttcgtt tacgctacgc cacccgcaca cagcaccgac cccatcgcga
      361 atgaatccgt gtccatccgt gttcatccgc ggttgagcgg ccttacctca cctgcaacgt
      421 gtcgccttcg aacgtgattc cgagcgaccg caccacgacc gccttccgcg cgccttcctt
      481 cttcaccgtg cccgccagcc acgcatcgta acccgtcgct ttcgccaccg cgagcaccgc
      541 gtcgacgttc tccggcgaca catacgccgc gaagccgatg ccctgattga acgtcgcata
      601 catctcgcgc tgcgagatcg gccccatctt ctccaaaaat ttgaacaccg ccagcggctc
      661 ccgcacgtgc tcgatctcgt agacgaacgg ctcatccagt cgcatcaact tccgccagcc
      721 gtgacccgtc acgtgcgcaa catagttcaa cttgatgccg cgttcctgac actcgcgcac
      781 gaacgccacg tagatcaccg acggcgccag cagcgcctcg ccaaacgtcc ggggatcgcc
      841 gaagcccacc ggcgtttggt atccgtccgg caaatgcgcc gccacgcgcc ggcacaacga
      901 aagcccgttc gtctgcacgc ccgagctcgc gaggaaaatg attttatcgc cttccttcac
      961 ctcacccgtg atccgcaaat ttttcggaga aattttcccg atcgccgaac ccgcgagcac
     1021 gatcgcctcg ggattcacca cgccgccgag cgtcggcgtt tcgccgccgc cccagaccgc
     1081 gcccgccgcc ttgcaacctt ctgcaaaacc atcgaccagc gccatcgtcc gcttttcgtc
     1141 gccaaaccag cccgagtcgc cc
//
LOCUS       DASPLT010000457         1308 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_5766, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000457 DASPLT010000000
VERSION     DASPLT010000457.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1308)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1308)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1308
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5766"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            85..>1308
                     /locus_tag="VEA63_04740"
     CDS             85..>1308
                     /locus_tag="VEA63_04740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HYD83329.1"
                     /translation="MPAVATDLLPTSLAAQLDEAQLQSVAEAPRDKRLGALADALKLS
                     EADALALLATAAGLPTASNLEADPSARGLLPARLVHDYQIIPIKLKAENSESRAESQT
                     TESSDSQLSALSSQLHLATAWLPDETMADWLRTFTARPPVWHLAVPERVHQLIIENYG
                     VGSGSLEDDDASYVAPEVQQQPEEAVDEEAAVVRFVSDVISQAVEDQATDIHFEPQEG
                     QLRIRYRVDGLLVPVPVPENLLRFQDAIISRLKIMARLNISERRLPQDGRINFRAENT
                     VLDIRVSTIPTIYAESISLRLLNQKKEAYTMDRLGMSAEEQSQITQILDYPHGIILVT
                     GPTGSGKSTSLNAFLRKINSTDLRIITIEDPIEYEVPGVNQMQVRPEIGLTFADALRH
                     VLRQDPDVIMVGEIRD"
BASE COUNT          284 a          499 c          309 g          216 t
ORIGIN      
        1 ataaaccagc acctcagcga ccccggcaaa aaagtgaaag agccgttgct cgaaaaattc
       61 aagtaagcgt ccgcgcctca ttccatgccc gctgtcgcca ccgaccttct tcccacatcg
      121 ctcgccgccc agctcgacga agcccagctc caatccgtcg ccgaagcacc gcgcgacaaa
      181 cgcctcggcg ctctcgccga cgcactgaaa ctttccgaag ccgacgcgct cgcgctcctc
      241 gccaccgccg ccggcctgcc caccgcgagc aatctcgaag ccgacccgag cgcgcgcggc
      301 ctgctccctg cgcgcctcgt tcacgattac cagatcatcc cgatcaaact caaagccgag
      361 aattcagagt ccagagctga gagccaaacc accgaaagtt cggactctca gctctcagct
      421 ctcagctctc aacttcactt ggccaccgcc tggctgcccg acgaaaccat ggccgactgg
      481 ctgcgcacgt tcaccgcgcg cccgcccgtc tggcacctcg ccgtgcccga acgcgttcac
      541 cagctcatca tcgagaacta cggcgtcggc tccggctcgc tcgaagacga cgacgccagc
      601 tacgtcgctc ccgaggtcca gcaacaaccc gaggaagccg tcgacgagga agccgccgtc
      661 gtccgcttcg tcagcgatgt gatctcgcaa gccgtcgaag accaggcaac cgacatccac
      721 ttcgaaccgc aggaaggtca gctccgcatt cgctaccgcg tcgacggctt gctcgtcccc
      781 gttccggtgc cggaaaacct gctacgcttt caagacgcga tcatctcgcg cctgaaaatc
      841 atggcgcggc tcaacatctc cgaacgccgc ctcccgcagg acggccgcat caacttccgc
      901 gccgagaaca ccgtcctcga tattcgcgtc tcgaccatcc ccacgatcta cgccgaatcc
      961 atctcgcttc gcctcctcaa ccaaaagaag gaagcctaca cgatggaccg cctcggcatg
     1021 agcgccgagg aacaatcgca gatcacgcag attctcgatt acccacacgg tatcattctc
     1081 gtcactggtc cgaccggctc cggcaaatcg acctcgctca acgcgtttct gcgcaaaatc
     1141 aattcgaccg atcttcgcat catcacgatc gaggatccga tcgaatacga agtccccggc
     1201 gtgaaccaga tgcaggtccg ccccgaaatc ggcctcacct tcgctgacgc actccgccac
     1261 gttctccgcc aagatcctga cgtcatcatg gtcggcgaaa tccgcgat
//
LOCUS       DASPLT010000458         2523 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_87572, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000458 DASPLT010000000
VERSION     DASPLT010000458.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 2523)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2523)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2523
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_87572"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1328)
                     /locus_tag="VEA63_04745"
     CDS             complement(<1..1328)
                     /locus_tag="VEA63_04745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD83330.1"
                     /translation="MKRWSFPRLPVLIGVIGLGAAVTSGTWAKRRIDARQQVEAAAIR
                     EEMKEIDAEVFAYRSRMANLRWNAAKMSEAVAIAEEPLKSWARERARRFAALVGEIER
                     ARKDADASLTGVGAEIEALCARAETAEARERLARLRPPKFPEAEEFLRLQAAHYLGPL
                     AQFSRQNPDYYRALKTFEPEASVEDVAALRRELAAAGGEVVTPQTMLGFELLSAVVPP
                     GDPLLADWATLVSAPDFFENPDPATVTRWRRAQKAVRAEDWPTAVAQMQAIVRSKVRT
                     RQPFRAAYGRAILRNTPDDAVTAYPFMQEAAAAGDVAARAWVSEEDFAKGKHDAALPW
                     LEARASDGETEAVAPLLVIYAMTADAVPRDAAREAATLQKIAVGPDAPAEALVLLARL
                     YESGRIERSAEKAFVCYQRAAEKGHIAAAIEAAERQLRGVGTTVDLDAARA"
     gene            1525..>2523
                     /gene="cysS"
                     /locus_tag="VEA63_04750"
     CDS             1525..>2523
                     /gene="cysS"
                     /locus_tag="VEA63_04750"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007359262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HYD83331.1"
                     /translation="MAIKLFDSLTREIRELRPSHPDGVYRFYNCGPTVYAPAHIGNFR
                     TFVVNDVLRRVLELEFGADKVKHVRNLTDVDDKTIKRARDEARPLAAVTQQWTEKFHA
                     DCAALNCLAPHVEPTATGHIREQVDMIDVLMRKGNAYRAADGSVYFKVSSFDGYGRLS
                     RVRERELQLGSALAGKSQAVDADEKEDGSDFALWKAHKPDDGDNAWDSPWGRGRPGWH
                     IECSAMSKKHLGETIDLHTGGVDLLFPHHENEIAQSECCNGTQFSRHWYHSEHLLVDG
                     KKMSKSLGNLYTLDELVAKGHSPMALRYALLAGHPRKQLNFTLDSLHAAESALATLR"
BASE COUNT          458 a          970 c          672 g          423 t
ORIGIN      
        1 gctcgcgccg cgtcgaggtc gaccgtagtc cccacaccgc gcagttgacg ctcggccgcc
       61 tcgatcgcag cggcgatgtg ccctttctcc gccgcgcgct gataacacac gaacgccttt
      121 tccgccgagc gctcgatgcg accgctctca tataaacgcg cgagcagcac gagcgcttcc
      181 gccggtgcgt ccggtccgac cgcgattttt tgcaacgtcg ccgcttcgcg cgcggcgtcg
      241 cgcggcaccg cgtccgctgt catcgcataa atcaccagca acggcgccac cgcctccgtc
      301 tcgccatcgc tcgcacgcgc ctcgagccac ggcagcgccg cgtcgtgttt ccctttcgca
      361 aagtcttcct cagaaaccca cgcccgcgca gccacatcgc ccgccgccgc cgcttcctgc
      421 atgaacggat acgccgtcac cgcgtcgtcc ggcgtgttgc gcagaatcgc gcgaccatac
      481 gccgcgcgaa aaggctgacg cgtccggacc ttcgagcgca caatcgcctg catctgcgcg
      541 accgccgtcg gccaatcctc cgcgcgcacc gccttttgcg cccgccgcca ccgtgtcacc
      601 gtcgcggggt ccggattctc gaaaaaatcc ggcgccgaca ccaacgtcgc ccaatccgcc
      661 aacaacggat cacccggcgg cacgaccgca ctcagcaatt cgaacccaag catggtttgc
      721 ggcgtcacca cctctcctcc cgccgcggca agttcacgcc ggagcgccgc cacgtcctct
      781 accgacgctt ccggctcgaa tgttttcagt gctcgataat agtccggatt ctgccggctg
      841 aactgcgcca gcggcccgag atagtgcgcc gcttgcaacc gcaaaaactc ttccgcctcg
      901 ggaaacttcg gcggccgcaa tcgcgccagc ctctcgcgcg cctcggccgt ttcagcgcgc
      961 gcacaaagcg cttcgatctc cgcgcccacg ccagtcaacg acgcatccgc gtcctttcgt
     1021 gcgcgctcga tctcccccac caatgccgcg aaacgccgcg cccgctcgcg cgcccacgat
     1081 ttcagcggct cttccgcgat cgcgaccgcc tcgctcattt tcgccgcatt ccaccgcaag
     1141 ttcgccattc gcgaccgata cgcgaacacc tccgcatcga tctctttcat ctcttcgcga
     1201 atcgccgccg cctcgacctg ttgccgcgcg tcgatccgcc gcttcgccca cgtcccgctc
     1261 gtcaccgccg cgcccagccc gatcacgcca atcaacacgg gcagccgcgg aaaactccag
     1321 cgcttcaccg tgacaggctt cgccgcgcct ccgcgggaag cggacgcaga tgtttcagaa
     1381 ggttgcgcca tgcgagttcg cctcctttat cggcgcaaat ctccgtccgc ttcaccgcca
     1441 cgcgccactt ctctcccgcg acataaaaac gctattccgc tctggcggaa aacccttcac
     1501 cgtccacctt cgtctctctc cttcatggcc atcaagctct tcgactcgct cacgcgagag
     1561 atccgcgaac tccgcccctc tcatcccgac ggcgtttacc gtttttataa ctgcggcccc
     1621 accgtctacg cgcccgcgca catcggcaat ttccgcacgt tcgtcgtcaa cgacgtcctc
     1681 cgccgcgtgc tcgagctcga gttcggcgcc gacaaggtga aacacgttcg caatctcacc
     1741 gacgtcgacg acaaaacgat caagcgcgcc cgcgacgaag cccgcccgct cgccgccgtg
     1801 acgcagcaat ggacggaaaa attccacgcc gattgcgccg cgctcaactg cctcgcaccg
     1861 cacgtcgaac ccaccgccac cggacatatc cgcgagcagg tcgacatgat cgacgtgctg
     1921 atgagaaagg gaaacgccta ccgcgccgcc gacggctccg tgtatttcaa ggtgtcatcg
     1981 tttgacggat acggccggct ttcgcgggtg agggagcgcg agctccagct cggcagcgcg
     2041 ctcgcgggta aatcgcaggc agtcgatgcc gacgagaaag aagacggcag cgattttgcg
     2101 ctctggaagg cgcacaaacc cgacgacggc gacaacgcct gggacagtcc gtggggccgc
     2161 ggccgccccg gctggcacat cgagtgcagc gcgatgagca agaagcatct cggcgaaacg
     2221 atcgatctgc acaccggcgg cgtcgacctg ctcttcccgc atcacgaaaa cgagatcgcg
     2281 cagagcgagt gctgtaatgg cacgcagttc tcgcgtcact ggtatcacag cgaacacctg
     2341 ctcgtggacg gcaagaagat gagcaaatcg ctcggcaatc tctacacgct ggacgaactc
     2401 gtcgcgaagg gtcactcgcc catggcgctc cgttatgcgc tgctcgccgg ccatccgcgc
     2461 aaacaactca acttcacgct cgactcgctg cacgcagcgg agagcgcgct cgccaccttg
     2521 cgc
//
LOCUS       DASPLT010000459         3493 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_229915, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000459 DASPLT010000000
VERSION     DASPLT010000459.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 3493)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3493)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3493
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_229915"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(16..657)
                     /locus_tag="VEA63_04755"
     CDS             complement(16..657)
                     /locus_tag="VEA63_04755"
                     /inference="COORDINATES: protein motif:HMM:NF016301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="paraquat-inducible protein A"
                     /protein_id="HYD83332.1"
                     /translation="MPSTSTHLTCPLCGQEHQPLALAAGERALCIRCETVLARGSRFG
                     RDATLLYALTGLALAVPALLLPFITAGKFGQEQGGLLFTGVEGLWDNGMPFLAIWVLL
                     CGALVPIALLAILAGGLLRTRLGWSQRPSDFLSRAAHAMGYWAIPEVQVLAVLVALMK
                     LGSVVSVSIGAGFWCYAGMSLSLLLAWRSFMLRAPEENSEPGAAPDESETVNA"
     gene            complement(829..1080)
                     /locus_tag="VEA63_04760"
     CDS             complement(829..1080)
                     /locus_tag="VEA63_04760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007358605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD83333.1"
                     /translation="MNYRTKAEYYIKGITQGFVDASEVIAWADEVIVAAPKTEDWMIE
                     ISTCGPDERMKVLSQLNTIKGDVDEAELKALLSAKGIQS"
     gene            1351..2370
                     /locus_tag="VEA63_04765"
     CDS             1351..2370
                     /locus_tag="VEA63_04765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD83334.1"
                     /translation="MENPDWADLGDEALLEKKIAHLGLTLEGTPLQAHVQQLYDELTA
                     KGLIFHPPCHVGDEWFVPVGIPAIFVPFFLVHERLRKLEAKMMLEVEGDNPEWFMRLM
                     RHEAAHAYAYAYQLYKKRKWQRVFGLTSTETTPQFYRPRPYSHSFVVHLDDWYAQSHP
                     DEDFAETFAVWLTPGLDWRARFNGWKALQKLEYVDELMRSLAGQPPPYQPAYRVRDFN
                     FLNIKLKTYYARKRKQYEDSYPDFYDRDLRQLFAGAVDAGPALKASLFLRQRKRALMD
                     AVCEWTNEKKYRVNKLLSRLIERCDQLDLHLKPGEPTPDLQVAAYIATLVMNYLFTGK
                     FKRTK"
     gene            2373..3392
                     /locus_tag="VEA63_04770"
     CDS             2373..3392
                     /locus_tag="VEA63_04770"
                     /inference="COORDINATES: protein motif:HMM:NF019121.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HYD83335.1"
                     /translation="MRRKLKVLALFDAVRPTTLDQDLSPELKTADWKTEAGVLAALNE
                     LGYPNEHLAIFDDLDLLRQKLQTFSPDVIFNLADQFRNNRAFDQHIVSFLAMCGIPFT
                     GCGSTGLTLCKHKGISKKILSYHRIHTPDFVIVPRGKRIVRPKRLKFPILVKPLKEEA
                     SYGISQASFVETDEQFRERVEFIHTKFDNDVIAEEYIEGRELYVSVIGNHRLQVFPIR
                     ELVFREVPPDEPKIATYKAKWDEAYRQRWGLENRFAEGLEPPLVRHIESVCKRIYHLL
                     TIDGYARLDLRLNAKNELYFIEANPNPILAPDEDFAQSAMKTGLSYPQLIDRIARLGL
                     STVRD"
BASE COUNT          720 a         1015 c         1119 g          639 t
ORIGIN      
        1 gaagggcgcg acggctcatg cgttcacggt ttcagactca tcgggcgcgg cgccgggttc
       61 gctgttttct tcgggcgcgc gcagcatgaa actccgccac gcgagcagca gcgacagcga
      121 catgccggcg taacaccaga agccggcgcc gatgctgaca ctgacgaccg agccgagctt
      181 catgagggcg acgagcaccg ccagcacctg gacttctgga atcgcccagt aacccatggc
      241 gtgggcggcg cgggaaagaa aatcgctcgg gcgttgcgac cagccgagcc gcgtgcgcaa
      301 caatccgccg gcgaggatgg cgagcagcgc gatcggcacg agtgctccgc agagcagcac
      361 ccagatcgcg agaaacggca tgccgttatc ccaaagtccc tcgacgccgg tgaacagcaa
      421 accgccctgt tcctggccga acttgccggc ggtgatgaag ggcagcagta gcgcgggaac
      481 ggcgagcgcc aggccggtga gcgcgtagag gagggtggcg tcgcggccga aacgcgaacc
      541 gcgcgcgagc acggtttcgc aacgaatgca caacgcccgc tcgccagccg cgagagcgag
      601 cggctgatgt tcctgtccgc acaacgggca ggtgaggtgc gtggacgtcg aaggcatggc
      661 aaaatgccac gcgagcgacc gtttaggacg ccatcggttc ccgggggacc tgcgtgctct
      721 caggtggaag cggtcggttc gcgcttgtcg cgccacgctg cgcgcgagcg cgcaacccac
      781 catttgctcg caggcgcgga ggcctgccgc tacgggaaaa gaccgtggtc aggactggat
      841 gcccttggcg ctgaggaggg ccttgagctc ggcttcgtcg acgtcgcctt tgatcgtgtt
      901 gagctggctg agaaccttca tccgttcgtc cgggccgcag gtcgaaatct cgatcatcca
      961 gtcctcggtt tttggcgcgg cgacgatcac ctcgtcggcc caggcgatca cctccgacgc
     1021 gtcgacgaaa ccctgggtga tgcccttgat gtaatactct gccttcgtgc ggtagttcat
     1081 ccgggaggta aggcagagcg ggttgcgggt gccaagcgtg attcgataga ggcggcgaac
     1141 gtaggtgctt tagcccgaat aaaacacact cgcgacctcg gcgagacagg tagggatgcc
     1201 tctccgaggc atccgcggac ggctcggaga gccgccccta ccgtcacgaa aacacttcat
     1261 cgggtgacct cgccggtctg cgcgcggcgg gacaatgagg aagcgcggct gcttgcttgt
     1321 aggtttccga aactcttcta gcgtcacggc atggaaaacc ccgattgggc tgatcttggc
     1381 gacgaagcgt tgctcgaaaa aaagatcgct catctcggac tcacgctcga agggacgccg
     1441 ctacaggcgc atgtgcagca gctctacgac gagctgacgg cgaaaggatt gatcttccat
     1501 ccgccgtgtc acgtcggcga cgaatggttc gtgccggtgg gcattcccgc gattttcgtg
     1561 ccgtttttcc tcgtgcacga gcggctgcga aaactcgagg cgaagatgat gctcgaagtc
     1621 gaaggcgata atccggagtg gttcatgcga ctcatgcggc acgaggcggc gcatgcgtat
     1681 gcttacgcgt atcaactcta caaaaaacgg aagtggcagc gcgtgttcgg gctgacttcg
     1741 accgagacga cgccgcaatt ttaccggccg cggccttaca gtcactcgtt cgtcgtgcat
     1801 ctcgacgatt ggtatgcgca gagtcatccg gatgaggatt tcgcggagac cttcgcggtg
     1861 tggctgacgc ccgggctcga ctggcgggcg cgcttcaacg gctggaaggc gctgcagaaa
     1921 ctcgaatacg tcgacgagct catgcgctcg ctcgcgggcc agccgccgcc gtatcagccg
     1981 gcgtatcgcg tgcgcgattt caattttctc aacatcaagc tgaagaccta ctacgcgcgg
     2041 aagcggaagc agtatgagga ttcgtatcca gatttctatg accgcgatct tcgccagctt
     2101 ttcgccggcg cggtggatgc ggggccggcg ttgaaggcgt cgctgtttct ccgacagcga
     2161 aagcgcgcgt tgatggatgc ggtttgcgag tggacgaatg aaaagaagta tcgcgtcaac
     2221 aagctgctct cgcgtctcat cgagcgttgc gatcagctcg acctgcatct gaagccgggc
     2281 gagccgacgc cggatctgca agtcgcggcc tacatcgcga cgctggtgat gaactacctt
     2341 ttcacgggaa aatttaaacg aacgaagtaa tcatgaggag aaagctgaaa gttctcgcgc
     2401 tgttcgatgc cgtgcggccc acgaccctgg atcaggatct gagccccgaa ttgaaaacgg
     2461 cggattggaa aacggaggcg ggcgtgctcg cggcgttgaa cgagctcggt tatccgaatg
     2521 agcacctcgc gattttcgac gacctcgatc tgctgcggca gaagctgcag acgttcagcc
     2581 ccgacgtgat tttcaatctc gccgaccagt ttcgaaacaa ccgcgcgttc gatcagcaca
     2641 tcgtgtcgtt tctcgcgatg tgcgggattc cgttcaccgg ctgcggctcg acggggctca
     2701 cgctttgcaa gcacaagggc atctcgaaaa agatcctgag ctaccatcgg attcacacgc
     2761 cggatttcgt gatcgtgccg cgcgggaaac gcatcgtgcg accgaagcgt ttgaagttcc
     2821 cgattctcgt gaagcctctg aaagaggagg cgtcttacgg catctcgcaa gcatcattcg
     2881 tcgagaccga cgagcagttt cgcgagcgcg tcgagtttat ccacacgaag ttcgacaacg
     2941 acgtgatcgc cgaggaatac atcgaagggc gcgagctcta cgtgagcgtg atcggcaatc
     3001 atcggctgca ggtgtttccg attcgcgagc tggtctttcg cgaggtgcca ccggacgagc
     3061 cgaagatcgc gacttacaag gcaaagtggg acgaagcgta tcgtcagcgt tggggcttgg
     3121 aaaatcgctt tgccgaaggc ctcgagccgc cgctcgtccg gcacatcgag tcggtgtgta
     3181 aacgcatcta tcacttgctg acgatcgacg gctacgcgcg gctcgatctg cggctgaacg
     3241 cgaagaacga actctatttc atcgaggcga atccgaatcc gatcctcgcg ccggacgagg
     3301 acttcgcgca gtccgcgatg aagacggggc taagttatcc gcagttgatc gaccggatcg
     3361 cgcggctggg gttgagcacg gtgagagact gacccgccgc aagcgaaggg aagctccaag
     3421 cgccaagatc caagctccag agaagatcca agaactgtca ccgcgaaagg cagggagcga
     3481 gctccgcgcg gtc
//
LOCUS       DASPLT010000460         1899 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_106919, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000460 DASPLT010000000
VERSION     DASPLT010000460.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1899)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1899)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1899
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_106919"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..588
                     /locus_tag="VEA63_04775"
     CDS             <1..588
                     /locus_tag="VEA63_04775"
                     /inference="COORDINATES: protein motif:HMM:NF045154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD83336.1"
                     /translation="ERISFNELMEIARQKTAPFLGPDPSGTRAAEEEAIVSRNLLQLY
                     TRGLVDIFTAPVPVTRSVPDKPQVTPLARYQALNARLVTSRAHAGLKFDAAGRLVISV
                     CDGTRDHASIANSLMDAVKSGKLRVAENGQPVEGEARLREVLTSLTEGHLKAIAMHGF
                     LTEREPSESAKIPSSATTTQLRVEEQPVAQPASTS"
     gene            complement(728..>1899)
                     /locus_tag="VEA63_04780"
     CDS             complement(728..>1899)
                     /locus_tag="VEA63_04780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009267 - cellular response to starvation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="carbon starvation CstA family protein"
                     /protein_id="HYD83337.1"
                     /translation="GVAIVVLAPMLKMPAVTQFIDGSGPVFAGPVFPFCFITIACAAI
                     SGFHALIASGTTPKLLAREKDIRVVGYGAMATEMIVGLMALIAACAMEPGQYFAINMR
                     GDAAMVTERITALGFPVTTSEMSALAADMGEKTMIGRAGGAPTFAAGMAQMFAGAFGG
                     GKAVALWYHFAIMFEALFILTTIDAGTRVGRFIVQDLLGMVAKPLGHTRSFAGNVLAT
                     LLFVGAWGWFLYQGVIDPLGGINSLWPIFGVANQLLAVIALALGTTVLIKMRRTRYVW
                     CTLAPLAWLLAVTMTAGWMKIFSADPRLGFLAAARDYAAKIAAGGATTAPEQVTQWER
                     LMFNQYVNAGVTGVFLVLVAIVVIANARVWWQLLGGRSAPELREERYVAVGGAAG"
BASE COUNT          388 a          721 c          500 g          290 t
ORIGIN      
        1 gagcgcatct ctttcaacga gctcatggaa attgcccgcc agaagaccgc gccttttctc
       61 ggtccagatc catccggaac ccgtgcggcg gaagaggagg ccattgtcag ccggaacttg
      121 ctgcagctct acacccgcgg gctcgtcgat atcttcacgg ctcccgtgcc ggtgacgcgg
      181 tccgtgccgg acaagcctca ggtgacgccg ctcgcgcgtt atcaggcgtt gaacgcccgc
      241 ctggttacga gtcgtgcgca cgcgggattg aagttcgacg ccgccggccg cctcgtgatc
      301 agcgtatgcg acggaacccg ggaccacgcc agcatcgcca attcgctgat ggacgccgtc
      361 aaatcgggaa agctgcgcgt cgcggaaaac ggtcaacccg tggaaggtga ggcgcggttg
      421 cgcgaagttc ttacctcact gaccgaaggt catttgaaag cgatcgcgat gcatggcttc
      481 ctcaccgagc gagaaccaag cgagtccgca aaaatccctt catccgccac cacaacccag
      541 ctccgcgtcg aagagcagcc ggtggcacag ccggcttcga cgagttgaat tttccccgag
      601 cgccgtcgca ccggtcagca tgtaaggcgc cccggccgct tcgagccgca cgataaaccg
      661 taagccaacc cccggcgcac gcaaggcccg ccgtttttcc gctgcgtccg cgccaagctg
      721 aacgacttca acccgccgcg ccgcccaccg ccacgtagcg ttcctcgcgc aactccggcg
      781 ccgaccgacc gccgagcagt tgccaccaca cgcgcgcgtt cgcgatcacc acgatcgcca
      841 cgagcaccag aaacacgccg gtcacaccgg cgttcacata ttggttgaac atcagccgct
      901 cccattgcgt cacctgctcc ggcgccgtag tcgctccgcc cgccgcgatc ttcgccgcat
      961 aatcccgcgc cgccgcgaga aaacccagcc gcggatccgc gctgaaaatc ttcatccatc
     1021 ccgccgtcat cgtcaccgcc agcaaccacg ccagcggcgc aagcgtgcac cacacataac
     1081 gcgttcgccg catcttgatc aacaccgtcg tcccgagcgc gagcgcgatc accgcaagca
     1141 gttggttcgc gacgccaaag atcggccaca gggaattgat cccgcccaat ggatcgatca
     1201 cgccctgata caagaaccat ccccacgcgc cgacgaacaa cagcgtcgcg agcacgttcc
     1261 ccgcaaacga tcgcgtgtgc cccaacggct tcgccaccat gccgagcaaa tcctgcacga
     1321 tgaaccgtcc cacgcgcgtc cccgcatcga tcgtcgtcag gatgaacagc gcctcgaaca
     1381 tgatcgcgaa gtgataccaa agcgccaccg ccttcccgcc gccaaacgcc cccgcaaaca
     1441 tctgcgccat ccccgccgca aacgtcggcg cgccaccggc gcgcccgatc atcgtcttct
     1501 cgcccatgtc cgccgcaagc gcgctcatct ccgacgtcgt caccggaaaa cccagcgccg
     1561 tgatccgctc ggtcaccatc gccgcgtcac cgcgcatgtt gatcgcgaaa tattgtcccg
     1621 gctccatcgc acacgccgcg atcagcgcca tgagccccac gatcatctcc gtcgccatcg
     1681 ctccgtagcc gaccacgcga atatcttttt cccgcgcgag cagcttcggc gtcgtgccgg
     1741 acgcgatcag cgcgtgaaat cccgagatcg ccgcacacgc gatcgtgatg aaacaaaacg
     1801 gaaacaccgg ccccgcgaac accggtccgc tcccgtcgat gaactgcgtc accgcgggca
     1861 tcttcaacat tggcgcgagc accacgatcg ccacgccga
//
LOCUS       DASPLT010000461         1702 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_170151, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000461 DASPLT010000000
VERSION     DASPLT010000461.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1702)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1702)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1702
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_170151"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1188)
                     /locus_tag="VEA63_04785"
     CDS             complement(<1..1188)
                     /locus_tag="VEA63_04785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NirA family protein"
                     /protein_id="HYD83338.1"
                     /translation="MSAPAAEPTAGFSQEQKQYLEGFLSALAAQAPFVGVNANGQLTA
                     NPSDASANLATPPAEDTVFGTPLGDLCKPERWKYDENPLDIWEKIVAHADADKFPDEA
                     DTFRFKYHGLFYVAPAQDAFMLRMRIPAGELTSHQLSGLAEIADWGGGYAHITTRANL
                     QIRELRPKDVVRVLVRLQELGLTSRGSGADNVRNVTASPNSGFDPDELIDVRPFAKGL
                     HHYILNHRDLYGLPRKFNVAFDNGGSIANATDTNDIGFMAVKVTEASLARAGGEAAAF
                     APGVYFRVQLGGISGHGDFARDTGLMVKPSECVALAAAMLRVFNEHGDRTNRKKARLK
                     YLLEKWGFEKFIEAAQAKLAFPLGRLPIDACEPRGREIKHGWVGVYKQSQRGLNSIGL
                     AIPV"
BASE COUNT          323 a          562 c          514 g          303 t
ORIGIN      
        1 cacgggaatc gcgagcccga tcgagttcag tccgcgctgc gactgtttat aaacaccgac
       61 ccagccatgc ttgatttcgc gaccgcgcgg ctcgcacgca tcgatcggca aacgcccgag
      121 cggaaacgcg agcttcgcct gcgccgcttc gatgaatttt tcgaaacccc atttctccag
      181 cagatatttc agccgcgctt tcttccggtt cgtgcgatcg ccgtgctcgt tgaacacgcg
      241 caacatcgcc gccgcgagcg cgacgcactc tgatggcttc accatcaaac ccgtgtcgcg
      301 cgcgaaatct ccgtgacccg aaatcccgcc gagctgcaca cgaaagtaga cacccggcgc
      361 aaacgccgcc gcttccccgc cggcgcgcgc gagcgacgct tcggtgacct tcaccgccat
      421 gaagccgatg tcgttcgtat ccgtcgcatt cgcgatcgag ccgccgttgt cgaacgcgac
      481 gttgaacttc cgcggcagac catagagatc gcggtgattg agaatataat ggtgaagccc
      541 cttcgcgaac ggccgcacgt cgatcaactc gtcgggatcg aagccgctgt tcggcgacgc
      601 ggtgacattg cgcacgttgt cggccccgga gccgcgcgac gtgagtccga gttcctgcaa
      661 acgcacgagc acgcgcacga cgtccttcgg gcgcaactcg cggatctgca ggtttgcccg
      721 cgtcgtgatg tgcgcgtagc cgccgcccca gtccgcgatt tcagcgaggc cggagagttg
      781 gtgcgacgtg agttcgcccg ccggaatgcg catgcgcagc atgaacgcat cctgcgccgg
      841 tgcgacatag aacaggccgt gatacttgaa acggaacgtg tcggcctcgt cgggaaactt
      901 gtcggcgtcc gcatgcgcga caattttctc ccagatatcg agcgggttct cgtcgtattt
      961 ccaacgctcg ggcttgcaga gatcgccgag cggcgtgcca aacaccgtgt cctccgccgg
     1021 cggcgtcgcg aggttcgcgc tcgcgtcgga aggattcgcc gtaagctggc cgttcgcatt
     1081 gacgccgacg aacggcgcct gcgcggcgag cgccgagagg aaaccctcga ggtattgctt
     1141 ctgctcctgg ctgaagccag cggtcggttc ggcagcgggt gcggacatga cggcgggaag
     1201 gtcggcgatt tagccgtgcg tgcgccgcag caacggcgcg tttctcgcgg gcgcgagcga
     1261 tgagcggtga gcggtgaaaa acgggcgcat gatcaccgtc tctttagccc acggcgtgcc
     1321 gtcctcgccc cacggataca gaaatgccga ccaaagtttt catcgctcgc atgaatttca
     1381 ttcatggcag agcgaacaac ctcgctttca gttcattgca gcagcctacc cgtccgcgta
     1441 gcggcagacc tccgtgcctg ccagttcgtc ggttcggaac tgtagggggt gcgctcgtcg
     1501 caccccgttg cgcgcaagcg cgcaacctac caccagcaga caggcacgga ggcctgccgc
     1561 tacgcaacgc cacaacagac gctacttcag catcagcgcc gccaccaagc ccgcaatcac
     1621 ggtgagcaac gcgaggcaca tcaactgata aaaccggatc ggattacgct ccagcagcgg
     1681 cgcgtccttg cggtgatacg gc
//
LOCUS       DASPLT010000462         3379 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_271594, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000462 DASPLT010000000
VERSION     DASPLT010000462.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 3379)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3379)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3379
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_271594"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1319)
                     /locus_tag="VEA63_04790"
     CDS             complement(<1..1319)
                     /locus_tag="VEA63_04790"
                     /inference="COORDINATES: protein motif:HMM:NF015052.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 57"
                     /protein_id="HYD83339.1"
                     /translation="MQPGLIFLPHGNLQYSQLRPDKRGWVALQSYGPLFDLSERQDAP
                     LAFEASGETLEIVAATAPAVLDKLKAGIREGRIEPVGSPHTHIMLANIDPEIGLESLR
                     DGLDTWERLTGVRPVTGWNPECSWASFIPEIFREAGYETLIGDADSYLLSSVEGLREA
                     TGLRFDVRGHSNKNALFKIEAEIAERPEILQALFRPNLLPNGLKVILRSDMLCNILLW
                     YLMGATEGNREHPIQPDEVRATLTRWRDRIPGGDGFLLPYAEDAEYVGTTAYFYVKQF
                     GLARFFEPAAESVTRFTDVLRMARALGFRLMTPREAVATYAAIPARGFESIENGCAWH
                     GGTAKAWANTPHARLLDPVCRSIHQGLKAVAAHLGIDEMRRDAEFHEVMRRITTAYVS
                     DARWPPQPTSPGRFNVEEALDAIVAANNGLEKIMTGRGLAAHRGLYSP"
     gene            1506..>3379
                     /locus_tag="VEA63_04795"
     CDS             1506..>3379
                     /locus_tag="VEA63_04795"
                     /inference="COORDINATES: protein motif:HMM:NF012686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD83340.1"
                     /translation="MFGFHPADFAVALLYIVLVCYFGIRGARRASKDQEGFFLAGRKL
                     GKVYQFFLNFGNSTDANGAVSAASLVYQQGVSGVWLGFQMVFLNPYYWFMNVWFRRVR
                     LVTTAELFVDRLGSQSLARFYALFQCLSAVVIIIGFGNLITYKISTALIVKPEATWTA
                     GERASVAAYHELQRYENVLKTTPLAALPADDQSRLVVLRERDAKGELRSTVSPIEPVS
                     FYLVYTLIVGAYVVLGGMAATAANEIIQSLLIVVFSAMLIPVGIHAVGGLEAFTAQVP
                     ATMFELLRDDGPSRQITALVLVGILANTLVQINGIAGNMAIYGSAKNEFAARFGGVSG
                     TFAKRLMIVMWAFCGLVAVALYQGPSALADPDEAWGAMSRQLLGPGFLGLMVVGVLAA
                     NMSTVAAQSMAVSALFVRSIYQPLRPAATEKESVFAGRVTIAVALILGVVAALSMNDV
                     FLMLQFMLTVNVPFGAAVLLMFFWRRLSVRAVWMAVAVSVLVTTVGPVVLPRLESVRT
                     NAALLERAQDETGRLHPVYFESVVRVDPNDDTSPLIGRDRLHLELWTLDLIGVPVEKM
                     SPSNQFAARLLFAALLPFVLLIGISCITRPPERSRIDAFFGKMKTPVGETPEADEAAM
                     A"
BASE COUNT          581 a         1154 c          974 g          670 t
ORIGIN      
        1 ggcgaataaa gcccgcgatg cgccgcgagg ccgcggcccg tcatgatttt ctccagcccg
       61 ttgttcgccg ccacgatcgc gtcgagcgct tcctcgacgt tgaagcggcc gggcgatgtc
      121 ggttgcggcg gccagcgcgc atcgctcacg taggcggtgg tgatgcgacg catcacttcg
      181 tgaaactccg cgtcgcgccg catctcgtcg atgccgaggt gtgccgccac cgccttcaat
      241 ccttggtgaa tgctgcgaca caccgggtcg agcagccgtg cgtgcggcgt gttcgcccac
      301 gccttcgccg tcccgccgtg ccacgcgcaa ccgttttcaa tgctctcaaa tccgcgcgct
      361 ggaatcgccg catacgtcgc gaccgcttcc cgcggcgtca tcaaacgaaa tcccaacgcg
      421 cgcgccatcc gcagcacgtc cgtgaaacgc gtgacgctct ccgccgccgg ttcgaagaag
      481 cgcgccagac cgaattgctt cacatagaaa tacgcggtcg tgccgacgta ttccgcatcc
      541 tccgcatagg gcagcaaaaa tccgtcgccg cccggaatgc gatcgcgcca gcgggtgagc
      601 gtcgcgcgca cttcgtccgg ctggatcggg tgctcgcgat ttccctcggt cgcgcccatg
      661 agataccaga gcagaatgtt gcacagcatg tcggagcgca ggatgacctt caggccgttg
      721 ggcagcagat tcggccggaa cagcgcttgc aaaatttcgg gacgctccgc gatctccgcc
      781 tcgatcttga acaacgcgtt cttgttgctg tgtcctcgaa cgtcgaagcg caacccggtc
      841 gcttcgcgca ggccctccac gctggacagc agatacgaat cggcgtcgcc gatcagcgtc
      901 tcataaccgg cctcgcgaaa aatctccgga atgaaactcg cccaactgca ctcgggattc
      961 caacccgtca ccggccgaac gccggtcagc cgctcccacg tgtcgagtcc atcgcgcagc
     1021 gactcgagcc caatctcggg atcgatgttc gcgagcatga tatgcgtgtg cggcgagccg
     1081 accggctcga tgcgaccttc gcgaatcccc gccttcaatt tgtcgagcac cgcgggtgcg
     1141 gtcgccgcga cgatctccag cgtctcaccc gacgcttcga aagcaagcgg cgcgtcctgt
     1201 cgctccgaaa gatcgaacaa aggaccatac gattgaagcg caacccagcc gcgtttgtcg
     1261 gggcgaagct gcgaatattg aagattgccg tggggaagaa agatgaggcc aggctgcatc
     1321 gagatgagag gtgaaactat gtcgcaaagg aacggcggcg atcagcgcag ccgccgcgag
     1381 tcgcggcgag gtcgcaacct gtgcaaggcc ttgcatggcc tcacggcttg ttcgtcgaat
     1441 gtcgttgagg ctcttctctc gcctctcctc gttgagcgct tcgctcccca ccctgcttca
     1501 ccctcatgtt tggatttcac cccgccgatt tcgccgtcgc cctgctctac atcgtgctcg
     1561 tttgctattt cggcattcgc ggcgcgcgac gcgcctccaa ggaccaggag ggtttttttc
     1621 tcgccggacg aaaactcggc aaggtctacc aattcttcct gaactttgga aattcgaccg
     1681 acgccaacgg cgccgtctcc gccgcgagtc tcgtttatca acaaggcgtc tccggcgtgt
     1741 ggctcggttt ccaaatggtt ttcctgaacc cttactactg gttcatgaat gtctggtttc
     1801 gccgcgtgcg actcgtcacc accgcggagt tgttcgtcga tcgcctcggc agccaaagtc
     1861 tcgctcgttt ctacgcgctg tttcagtgcc tctccgcggt cgtcatcatc atcggcttcg
     1921 gcaatctcat cacctacaaa atctccaccg cgctgatcgt gaaacctgaa gcgacgtgga
     1981 ccgccggaga gcgcgcgtcc gtcgccgcgt atcacgaact gcagcgctac gaaaacgtgc
     2041 tcaaaaccac accgctcgcc gcgctccccg ccgacgatca atcccgcctc gtcgtcctcc
     2101 gcgaacgcga cgcgaaaggc gaattgcgga gcaccgtatc gccgatcgag cccgtttcat
     2161 tttacctcgt ctacaccttg atcgtcggcg cctacgtcgt gctcggcggc atggccgcga
     2221 ccgccgccaa cgagatcatc caaagcctgc tcatcgtcgt tttttccgcg atgctgattc
     2281 ccgtcggcat ccatgcggtg ggcgggctcg aggcgttcac cgcgcaagtt ccggcaacga
     2341 tgttcgaact tctccgcgac gatggaccct cgcgccagat cacggcgttg gtgctcgtcg
     2401 gcatcctcgc caacacgctc gttcagatca acggcatcgc cggcaacatg gcgatctacg
     2461 gctcggcgaa aaacgaattc gccgcgcgct tcggcggcgt gagcggcacg ttcgcaaagc
     2521 gactcatgat cgtcatgtgg gctttctgcg gcctcgtcgc cgtcgcgctc taccagggcc
     2581 cgtccgcgct cgccgatccc gacgaagcct ggggcgcaat gtcgcgacaa ctgctcgggc
     2641 caggtttcct tggcctgatg gtcgtcggcg tgctcgcggc gaacatgtcc actgtcgctg
     2701 cgcaatcgat ggcggtctcc gcgctcttcg tgcgcagtat ctaccaaccg ctgcgcccgg
     2761 ccgcgacgga gaaggaaagc gtcttcgccg gccgcgtcac catcgccgtc gcgctgatcc
     2821 tcggcgtcgt cgccgcgctc agcatgaacg acgtttttct catgctgcag ttcatgctta
     2881 cggttaacgt gccgttcggc gctgccgtgc tgctgatgtt tttctggcga cggttgagcg
     2941 tccgcgccgt ctggatggcc gtcgccgttt cagtcctcgt gaccaccgtc ggcccggttg
     3001 tcctgccgcg cctcgagtcg gtgcgcacga acgccgcgct gctcgaacgc gcacaagacg
     3061 aaaccggccg cttgcatccc gtctatttcg aatccgtcgt gcgcgtcgat ccgaacgacg
     3121 acaccagccc gctcatcggt cgcgaccgac tgcatctcga actctggacg ctcgacctca
     3181 tcggcgtgcc ggtggaaaaa atgtcgccga gcaatcaatt cgccgcgcgc ctgctgttcg
     3241 ccgcgctgct gccgttcgtc ctgctcatcg gcatcagttg catcacgcgt ccgccggagc
     3301 gcagtcgcat cgacgcgttc ttcggcaaga tgaaaacccc ggtcggcgaa acgccggagg
     3361 cggacgaagc cgcgatggc
//
LOCUS       DASPLT010000463         1558 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_45213, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000463 DASPLT010000000
VERSION     DASPLT010000463.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1558)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1558)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1558
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_45213"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            63..>1558
                     /locus_tag="VEA63_04800"
     CDS             63..>1558
                     /locus_tag="VEA63_04800"
                     /inference="COORDINATES: protein motif:HMM:NF012935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HYD83341.1"
                     /translation="MSASVAPISPLSAPTVSLGRIAHTFDAIVIGSGMSGGWAAKELC
                     EKGLKTLVLERGRNVEHITHYPTAMLEPWQMPHRGALSPEFKRQNPILSQCYAADGST
                     QHFFVKDAEHPYEQTKPFSWIRGYQVGGKSLMWARWTQRWSDLDFEANAREGIAVDWP
                     LRYADLAPWYSYVEKFVGISGNRDGLPQIPDGEFLPPMALNCVEQHMKETIESRWPGR
                     HLVISRSANLTRPIEGRGVCMYRDRCSRGCPFGGYFSANSATLPAAAATGNLTLRPDS
                     VVHSLIYDEQKNRATGVRVIDAHTKQITEYFAKIIFVNASTLNTSLILLNSTSSRFPN
                     GLGNDSGQLGRNLMDHNYRVKVTGRHEGFQDKYYYGRRPTGALLPRFRNVGSDRQTGF
                     LRGYAFSVFGGRGRGNHDDSTPPIGAEFKKKSAELGDWNLWMNCMAEHLPHYDNKITL
                     SKTSTDAWGMPTMIIDCEHKENELAMTKDMLMTAAEMAEASGFTNVRA"
BASE COUNT          309 a          590 c          388 g          271 t
ORIGIN      
        1 ctttctccgc ccccgcgcgt caccctcccg cgcccgcctt ccccgcccct ctccgattcc
       61 ccatgtccgc ctccgtcgcc cccatttctc cgctctccgc gcccaccgtc tcgctcggca
      121 gaatcgccca caccttcgac gccatcgtca tcggctccgg catgtccggc ggctgggccg
      181 ccaaggaact ctgcgagaaa ggcctcaaaa ccctcgtcct cgagcgcggc cgaaacgtcg
      241 aacacatcac gcactacccc accgccatgc tcgagccatg gcagatgccg caccgcggcg
      301 ccctctcgcc cgagttcaaa cgccaaaatc cgatcctctc ccaatgctac gccgccgacg
      361 gttcgacgca gcacttcttc gtcaaagacg ccgagcaccc ttacgagcaa acgaaaccat
      421 tttcctggat cagaggctac caagtcggcg gcaaatccct catgtgggct cgctggacgc
      481 agcgctggag cgatctcgat ttcgaagcca acgctcgcga gggcatcgcc gtcgactggc
      541 ccctccgcta cgccgacctc gcgccgtggt attcctatgt cgagaaattc gtcggcatca
      601 gcggcaatcg cgacggcctt ccgcaaattc ccgacggtga gtttctcccg ccgatggcgc
      661 tcaactgcgt cgagcaacac atgaaggaga ccatcgagtc gcgctggcct ggccgccatc
      721 tcgtcatctc ccgttccgcc aacctcacgc gcccgattga gggccgcggc gtctgtatgt
      781 atcgcgatcg ctgcagccgc ggctgccctt tcggcggtta cttctccgcc aactccgcca
      841 cgctgcccgc cgccgccgcc actggcaatc tcaccctccg gcccgactcc gtcgttcact
      901 cgctcatcta cgacgaacaa aagaaccgcg cgaccggcgt gcgcgtcatc gacgctcaca
      961 cgaagcaaat caccgaatac ttcgcgaaaa tcatcttcgt caacgccagc accctcaaca
     1021 cgtccctcat ccttctcaac tccacgtcct cgcgttttcc gaacggcctc ggtaacgaca
     1081 gcggccagct cggccgcaat ctcatggatc acaattaccg cgtgaaagtc accggccgcc
     1141 acgagggttt tcaggataaa tattactacg gccgccgccc caccggcgcg cttctcccgc
     1201 gtttccgcaa cgtcggcagc gatcgacaaa ccggtttcct ccgcggctac gccttctctg
     1261 ttttcggcgg acgcggacgc ggcaatcacg acgactccac tccaccgatc ggcgccgaat
     1321 tcaaaaagaa gagcgccgag ctcggcgact ggaacctttg gatgaactgc atggccgagc
     1381 acctgccgca ctacgacaac aagatcacgc tgagcaaaac cagcacggac gcgtggggca
     1441 tgccgacaat gatcatcgac tgcgaacaca aggagaacga gctcgcgatg accaaggaca
     1501 tgctgatgac ggcggcggaa atggctgagg cttcaggctt caccaacgtt cgcgcctg
//
LOCUS       DASPLT010000464         1151 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_5849, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000464 DASPLT010000000
VERSION     DASPLT010000464.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1151)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1151)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1151
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5849"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            334..777
                     /gene="ribH"
                     /locus_tag="VEA63_04805"
     CDS             334..777
                     /gene="ribH"
                     /locus_tag="VEA63_04805"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009508958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HYD83342.1"
                     /translation="MDGAAIRVGIVAARFNGDLVDALLARVVAELRAAGVKEKALTVV
                     RVPGSHEVPWAAGELARSGRRDVVIGLGVLIGGETSHHELVGESVSHALQSVALTTRV
                     PVINGVIVTDTRAQAAARCTGRINRGAEFGAAALEMAALKRTLSR"
     gene            774..>1151
                     /gene="nusB"
                     /locus_tag="VEA63_04810"
     CDS             774..>1151
                     /gene="nusB"
                     /locus_tag="VEA63_04810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007359643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HYD83343.1"
                     /translation="MSKAQFAQRRDGRVAALQYLFAWSMNSPKNLAEDLRVFFENMDK
                     PRDHYAFGEELIQGVILNVDEIDARIKGLAHNWEFERIAKIDLAILRLAMFEMIHRKD
                     IPPVVSINEAIDLSKEFSNADAKR"
BASE COUNT          222 a          297 c          413 g          219 t
ORIGIN      
        1 cggcgacaat gacggaaaga acggcacagt atacttgctt gggggaaggc atggcgggaa
       61 acacgggaaa gaggcgtgcc gttatcgtgg gtaggaggtt gttcgccgca aggcgggtga
      121 attctacagg ttagagcgag cgggcgtgtc gcgaggccgt ttgtctgatg ctggcggcgt
      181 tcgaagcagc gcgaatcgcg ttcgtttctc gctcggcggg atacgggtga gcgatggaga
      241 ctgaacgggc cgcgcgggat ttgagttgcg gagatgaggt ttggcgaagt cgatagccgg
      301 ttcatgagtc tggctgctcc tagtcgaaga ccgatggatg gtgccgcgat ccgcgtcggc
      361 attgtcgcgg cacgtttcaa tggcgacctc gtcgatgcgt tgctcgcgcg ggtcgtggcg
      421 gaattgcgtg cggctggcgt gaaggaaaaa gcgctgacag tggtgcgcgt gccgggctcg
      481 cacgaagtgc cgtgggcggc aggtgagctc gcgcgcagcg ggcgacgcga tgtggtgatc
      541 gggctcggcg tattgatcgg cggcgaaacg tcgcatcacg agctggtggg cgaaagcgtc
      601 tcgcatgcgt tgcagagtgt ggcactcacc acgcgtgtgc cggtcatcaa tggggtgatt
      661 gtgaccgata ctcgcgcgca ggccgcagct cgttgcaccg gacggatcaa ccgcggcgcg
      721 gagtttggcg cggcggcgtt ggagatggcg gcgttgaaac gaaccctttc ccgatgagca
      781 aagcgcaatt cgcccagcga cgcgacggcc gggtcgccgc gctccagtat cttttcgcgt
      841 ggagcatgaa ctccccgaag aacctcgcag aggatctgcg ggttttcttc gaaaacatgg
      901 acaagccccg cgatcactac gcgttcggcg aggagctgat ccaaggcgtg atcctgaacg
      961 tcgacgaaat cgacgcgcgt atcaaaggcc tcgcgcacaa ctgggagttc gagcgcattg
     1021 cgaaaatcga tctcgcgatc ctgcggctcg cgatgttcga gatgatccat cggaaggaca
     1081 tcccgccggt ggtctcgatc aatgaagcga tcgatctttc gaaagagttt tcgaatgccg
     1141 acgcgaagcg c
//
LOCUS       DASPLT010000465         1047 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_128365, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000465 DASPLT010000000
VERSION     DASPLT010000465.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1047)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1047)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1047
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_128365"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..484)
                     /locus_tag="VEA63_04815"
     CDS             complement(<1..484)
                     /locus_tag="VEA63_04815"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HYD83344.1"
                     /translation="MKPLRILLVDDEPETGAALEAWLKRQGYESTFANGPEAADRAFA
                     TQEFDVVLSDIQMPGNFKLEWIARRLEAACPPPILLMTGSPELETAMRAANLPVAGYL
                     LKPLFFEEAAATIERLGTDHRHRRELLALSHEVTRLLNSHAPHSVSDPFTEELHRLAQ
                     Q"
     gene            complement(519..1022)
                     /locus_tag="VEA63_04820"
     CDS             complement(519..1022)
                     /locus_tag="VEA63_04820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HYD83345.1"
                     /translation="MSIATPTAGSASKLAGKYLTVVLENEAYGIAVLKVREIIRMQKI
                     TPVPQMPSFVKGVINLRGRVIPVVDLRVKFGLKAEFAERTCIVVVQVKLPSEQIVQMG
                     LIVDSVEEVVTLANDEIEPTPDFGTKIDTTYLLGMAKVKGQVKTLLDIDRVVAPETVQ
                     AIAQVAP"
BASE COUNT          178 a          283 c          387 g          199 t
ORIGIN      
        1 gctgttgcgc gaggcggtgc aactcttcgg tgaacgggtc ggagacgctg tgcggcgcgt
       61 gcgagttcag caagcgcgtg acctcgtgcg agagagcgag cagttcgcga cgatggcgat
      121 ggtcggtgcc gagacgctcg atcgtggcgg cggcttcctc gaaaaaaagc ggtttcaaca
      181 gatagccggc gaccggcaga tttgcggcgc gcatcgcggt ctcgagttcc ggactgccgg
      241 tcatcaggag gatgggcggc gggcacgcgg cttcgagccg gcgggcgatc cactccagct
      301 tgaagttgcc gggcatctgg atgtcgctga gcacgacatc aaattcctgg gtcgcgaacg
      361 cgcgatcggc ggcttcggga ccgttcgcga aggtgctctc gtaaccctgg cgtttgagcc
      421 aggcttcgag cgcggcgccg gtctcgggtt cgtcgtcgac cagcaggatg cgcagtggtt
      481 tcactggcgg ggaaaacggc ggcgagccgg aggcaaaatc agggcgcgac ttgcgcgatc
      541 gcctggacgg tttccggcgc gacgacgcgg tcgatgtcga ggagggtttt cacctggcct
      601 ttgaccttgg ccatgccgag caggtaagtg gtgtcgatct tcgtgccgaa gtcgggcgtg
      661 ggctcgatct cgtcgttggc gagcgtgacg acttcctcga cgctgtcgac aatgaggccc
      721 atttgcacga tctgctcgga gggcagcttg acctggacga cgacgatgca cgtgcgctcg
      781 gcgaactcgg ctttgaggcc gaacttgacg cgcaggtcga cgaccgggat gacgcggccg
      841 cggaggttga tcacgccctt cacgaagctc ggcatttgcg gaaccggcgt gatcttctgc
      901 atgcggatga tttcgcgcac cttgaggacg gcgatgccat aggcttcgtt ctcgagaacg
      961 accgtgaggt atttgccggc gagtttcgaa gcggagcctg cagtgggagt ggcgatggac
     1021 atggcgggag agaagttggt gaacggg
//
LOCUS       DASPLT010000466         2171 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_5910, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000466 DASPLT010000000
VERSION     DASPLT010000466.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 2171)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2171)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2171
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5910"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1541)
                     /locus_tag="VEA63_04825"
     CDS             complement(<1..1541)
                     /locus_tag="VEA63_04825"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HYD83346.1"
                     /translation="MNTSSLRRVMPALVLTLALAPAFVIRAQTPAPPPPEPVAAAVGT
                     EQPIVLNTFTVDVTRDRGYVAVDSLAGGRTNTQIKLTPAAMSSLTRTFLDDLGIQDVR
                     QALRWSPNVLPEDPLAGRGFGGQAFHPWAFSFRGVGSGQQGGAGPTRNYFTFFENADA
                     YNIERVEFTRGPNGILFGLGTVGGTLSTYTKVPRLDQTFISPVVVADSHGSLRFELDY
                     NLAASEKLALRVNALHDSVKGWRNGDQGTKRAVDLAALYKFNDRTSVRIEAEGYRSKY
                     SLISTTIGDKASGWDGQTASDTWGALPTGSSRTVKMSVPGAWGEGLQAYPVFVPTLPG
                     NQRLMLWAPWSMDPDPENPGQLVRTYHGGYASTSALRDVGQALNWAPYPGWYPDQIKL
                     PWETEYSSTANVPIRPSREWTYGAGRGTNDYENLSVFFDHSFNENIDLQVAFFTYDTE
                     TTSKDYEGTGGASIDINRQLPDGSPNPNFGKTFADFFLSRQTQNRSVDEIRAQLNYKF
                     DGELFG"
     gene            complement(1623..>2171)
                     /locus_tag="VEA63_04830"
     CDS             complement(1623..>2171)
                     /locus_tag="VEA63_04830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD83347.1"
                     /translation="GFSVPDSKIVESMPGQRAYFAEFLPTWDSVANGSTPEATQLRTL
                     LTNAQIRLDENTAGSTKTGLVDYTANVFANYTFVGDRFDGLSIGAGAARTGAQYLNTI
                     DDEMIYSSVRTTVNAVIAYEFDFRDIGVRLALNVENLLNETDPIITSYDGAWKDGRGN
                     PIPNGYYFQTPRTWRLTARLSF"
BASE COUNT          368 a          574 c          800 g          429 t
ORIGIN      
        1 ccgaaaagtt cgccgtcgaa cttgtagttg agctgcgcgc gaatctcgtc gacggagcgg
       61 ttttgcgtct gccgcgagag gaaaaaatcg gcgaaggttt tgccgaagtt gggattcggc
      121 gagccgtcgg ggagctggcg attgatgtcg atcgacgcgc cgccggtgcc ttcgtaatcc
      181 ttcgatgtcg tctcggtatc gtaggtgaag aacgcgactt gaagatcgat gttttcgttg
      241 aagctgtggt cgaaaaagac gctgaggttt tcgtagtcgt tcgtgccgcg acccgcgccg
      301 taagtccact cgcgactcgg gcgaatcgga acgttggcgg tggaggagta ttcggtttcc
      361 cacggcagtt tgatctgatc gggataccaa ccgggatagg gcgcccagtt cagcgcctgg
      421 ccgacgtcgc gcaacgcgct ggtcgatgcg tagccgccgt gatacgtgcg caccaattgc
      481 ccgggatttt ccggatcagg gtccatcgac cacggggccc agagcatcaa ccgctgatta
      541 ccgggcagtg ttggcacgaa tacgggatag gcttggagac cttcgcccca agcgccggga
      601 acagacattt tcacggtgcg cgagctgccg gtgggcaggg cgccccacgt gtccgaagcg
      661 gtttgcccat cccaacccga tgccttgtcg ccgatggtgg tcgagatcag gctgtatttg
      721 gagcgataac cttccgcttc gatgcggacg ctggtgcgat cgttgaactt gtagagtgcg
      781 gcgagatcga cggcgcgttt cgtgccttga tcgccgttgc gccagccctt gacggagtcg
      841 tgcaaggcgt tcacgcggag cgcgagtttt tcgctcgcgg cgaggttgta atcgagttcg
      901 aagcgcaggc tgccgtggct gtcggcgacg acgacggggc tgatgaacgt ttggtccagc
      961 cgcgggactt tcgtgtaggt ggacaacgtg ccgccgacgg tgccgaggcc gaaaaggatg
     1021 ccgttgggac cgcgcgtgaa ttcgacgcgt tcgatgttat aggcgtcggc gttttcgaaa
     1081 aacgtgaagt agttacgcgt cgggccggcg ccgccttgct ggccggaacc gacaccgcgg
     1141 aaactgaacg cccacgggtg aaacgcctgg ccgccgaaac cgcggccggc gagcgggtct
     1201 tcggggagaa cattcggcga ccagcggagc gcttggcgca cgtcctggat cccgaggtcg
     1261 tcgagaaacg tgcgcgtcaa cgacgacatc gccgcgggcg tgagcttgat ctgcgtgttc
     1321 gtgcgaccgc cggcgagcga gtcgaccgcg acgtagccac ggtcgcgcgt gacgtcgacc
     1381 gtgaaggtgt tgaggacgat cggttgctcc gtgccgacgg cggcggccac gggttcaggt
     1441 ggcggcggcg cgggcgtttg cgcgcggata acgaacgccg gcgcgagtgc cagcgtgaga
     1501 acgagcgccg gcatgacgcg gcgtagagac gaggtgttca tgggtgggtg tagtcggtgt
     1561 gttcatgctg gaaaattgga tgtgccatgg gcgacgcggg gcgaccgcgt tgccgtttcg
     1621 ttttagaacg agaggcgggc ggtgagccgc caggtgcgcg gcgtctggaa atagtagccg
     1681 ttggggatgg ggttgcccct gccgtccttc cacgcgccgt cgtagctggt gatgatcggg
     1741 tcggtttcgt tgaggagatt ctcgacgttg agtgcgaggc ggacgccgat gtcgcggaag
     1801 tcgaattcgt aggcgatgac ggcgttgacc gtcgtgcgga cggacgagta gatcatttcg
     1861 tcgtcgatgg tgttgaggta ttgtgcgccg gtgcgcgccg cgccggcgcc gatcgagagg
     1921 ccatcgaagc gatcgccgac gaacgtatag ttcgcgaaga cgttggcggt gtagtcgacg
     1981 aggccggtct tggtggagcc ggcggtgttt tcgtcgaggc gaatctgcgc gttggtcagc
     2041 agcgtgcgga gttgggtggc ttcgggcgtg gagccgttcg cgacggagtc ccacgtgggg
     2101 aggaattccg cgaaataggc gcgctggccg ggcatcgatt cgacgatctt ggaatccggg
     2161 acgctgaagc c
//
LOCUS       DASPLT010000467         2674 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_148841, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000467 DASPLT010000000
VERSION     DASPLT010000467.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 2674)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2674)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2674
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_148841"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1151)
                     /locus_tag="VEA63_04835"
     CDS             complement(<1..1151)
                     /locus_tag="VEA63_04835"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007362750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HYD83348.1"
                     /translation="MAQPPFSYQDPFPLGADETEYRLLSKEGVTTATFEGQEILKVAP
                     EALAFLAQQAFHDCSFMLRPKHLQQVAAILDDPTASENDRYVALTLLKNAEVAAQGIL
                     PFCQDTGTATIVAKKGQQVWTGANDAEWLSRGVYECYTKENLRYSQTAPLDLWKETNT
                     NTNLPAQIDLYATEGASYKFLFVAKGGGSANKTFLFQETKALLNPKSFEKFVADKLGY
                     LGTAACPPYHLVFVIGGTSAEACLKTVKLASAKYLDALPTTGNEHGRAFRDLAMEAKI
                     LEIAQQSGIGAQFGGKYFALDVRVVRLPRHGASCPVGLGVSCSADRNIKGKITKDGIW
                     LEQMEANPGRFIPADQRTLKEDNIVRIDLNQPMDKIRAELSRHPVTTRVS"
     gene            complement(1421..>2674)
                     /locus_tag="VEA63_04840"
     CDS             complement(1421..>2674)
                     /locus_tag="VEA63_04840"
                     /inference="COORDINATES: protein motif:HMM:NF033542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HYD83349.1"
                     /translation="RQALYVPSMRGRDHDWKRLREWAEELVAAGDSSHGEDLAALTEE
                     GGRPGRSAEGWPAIGAAGSVKPRQAKRHLDLVTEPWLPIWPWAKLEPLTEPQSSHRKK
                     ESNMSAELNRPIVRYAGLDISKPHLDFDLGAAQRGRLPHNVTGQGQLIETVRGYRVVC
                     EASGGYEARVVAALLEAGIEVCVVMPGRVRHFAKAEGLEAKTDRIDAALLRRFGEKLQ
                     PRLSVPANAEQQALRELLEYRRLVVTHLTEVEQRLELAGPTLAPLLRRQQSQGQKELT
                     RIDRHITQHVARHETLRTTAARFQQLCGVGPVLAASLLAYVPELGRISSQQLCALVGV
                     APHPRDSGLTRRARHVRGGRDTVRHVLYMAAVCAAHHNPLLKAFYERLTARGKPKKVA
                     LVAVMRKMLCVLHKLVAEPDFVLAS"
BASE COUNT          444 a          799 c          885 g          546 t
ORIGIN      
        1 gacacgcgtg tcgtcaccgg atgccgtgag agctccgcgc gaattttgtc catcggctgg
       61 ttgaggtcga tcctcacgat gttgtcttcc ttcaacgtgc gttgatcggc ggggataaaa
      121 cgtccgggat tcgcctccat ctgctcgagc cagatgccat ccttcgtgat cttgcccttg
      181 atgttgcgat cggcgctgca cgaaacgccg aggccgaccg ggcagctcgc gccgtgacgc
      241 ggcaagcgga cgacgcgcac gtcgagcgcg aaatattttc cgccgaactg cgcgccgatg
      301 ccgctctgtt gcgcaatctc gagaatcttc gcctccatcg cgaggtcgcg aaacgcccgt
      361 ccatgctcgt taccggtggt cggcagcgca tcgaggtatt tcgcggacgc gagcttcacg
      421 gttttcaggc aggcttccgc gctcgtgccg ccgatgacga ataccaggtg atacggcggg
      481 cacgcggcgg tgccgagata gccgagcttg tcagcgacga atttctcgaa gctcttcgga
      541 ttaagaagcg ccttcgtttc ctgaaagaga aacgtcttgt tcgcggagcc gccgcctttc
      601 gcgacgaaga ggaatttgta gctcgcgcct tcggtggcgt agagatcgat ctgcgcggga
      661 aggttcgtgt tggtgttcgt ttccttccag aggtcgagtg gcgccgtttg cgagtagcgc
      721 aggttctcct tcgtgtaaca ctcgtagacg ccgcggctca accattcggc atcgttcgcg
      781 ccggtccaga cttgctggcc cttctttgcg acgatcgtgg ccgtgccggt gtcctggcag
      841 aacggcagga tgccctgcgc cgcgacctcc gcgttcttga ggagcgtgag cgcaacatag
      901 cggtcgtttt cggatgcggt tggatcgtcg agaatcgcag caacttgctg caggtgtttc
      961 ggccggagca tgaacgagca gtcgtgaaac gcctgctggg cgagaaacgc gagcgcttcg
     1021 ggcgcaacct tcaggatctc ctgtccttcg aacgtcgcgg tggtcacgcc ttctttcgaa
     1081 agcaggcgat attcggtttc atccgcgccg agcggaaagg ggtcttggta ggagaacggc
     1141 ggttgagcca tggcgcctca catcctgcgt ggcgagcgga cggactcaag aacgcgccgt
     1201 gccggcaaag cgcgtagcag cgcggctttt gggcgcgcaa cgtgcgatca tacgccggcg
     1261 agtagctgcg ttgcggggat ttccgtgcat gcctctctgt ttgtctgtct gcctgtcgct
     1321 cggttcggaa tgtaggtggt gcgcttgtcg caccacgtgg cgctgagcgc tcgagcgggc
     1381 gacctatcac caaagcaggc aggcgcggag cagcggtgtc tcatgaggca aggacgaaat
     1441 cgggttcggc gacgagctta tggaggacac aaagcatttt gcgcatgacg gcgacgaggg
     1501 cgactttctt gggcttaccg cgagcagtca ggcgttcgta aaacgctttg aggagcggat
     1561 tgtgatgagc ggcgcagaca gcagccatgt agaggacgtg gcgcacggtg tcgcgtccgc
     1621 cgcgcacgtg gcgggcgcgt cgagtcaggc cgctgtcgcg gggatgcggc gcgacgccga
     1681 ccaaggcaca cagttgttgc gacgagatgc ggcccagttc cgggacgtaa gcgagcaagc
     1741 tggccgcgag caccggtccg acgccgcaca gttgctggaa ccgcgcggcg gtggtgcgta
     1801 gggtttcatg ccgggcgacg tgctgggtga tgtggcggtc gatccgggtg agttcctttt
     1861 gcccttggct ttgctggcgg cgcaacaggg gcgccagcgt gggaccggcc agttcgagtc
     1921 gttgctcgac ctcggtgagg tgagtgacca ccaaccggcg atactccagc agttcgcgca
     1981 acgcttgctg ctcggcattg gccggcacgc tcaaccgggg ttggagtttc tcgccgaaac
     2041 ggcgcagcaa cgccgcatcg attcggtccg tctttgcttc caaaccctcg gccttggcga
     2101 agtggcgcac ccggccaggc atcaccacac agacttcgat tccagcttcg agcagggcgg
     2161 caacgaccct tgcttcgtag cctccggacg cttcgcagac gacgcggtat ccgcgcaccg
     2221 tctcgatgag ttgaccttgt ccagtgacgt tatggggcag gcggccccgc tgggcagcac
     2281 ccagatcaaa atcgaggtga ggcttgctga tatccagtcc ggcgtagcgg acgatcgggc
     2341 ggttcagttc ggcagacatg tttgactcct tcttgcgatg cgagctctgt ggctctgtca
     2401 gcggttcgag ttttgcccag ggccagatcg gcagccacgg ctccgtgacg aggtctaaat
     2461 gcctcttggc ctgcctcggc ttgaccgatc cggcagcgcc gatggccggc cagccctcgg
     2521 cgctcctccc tggacggccg ccctcttcgg tgagggcggc caagtcctca ccatgactgg
     2581 aatcgcctgc tgcgacgagc tcttcggccc attctcgcag gcgcttccag tcatggtctc
     2641 tccctctcat cgaggggaca tacaaggcct gccg
//
LOCUS       DASPLT010000468         1071 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_128544, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000468 DASPLT010000000
VERSION     DASPLT010000468.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1071)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1071)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1071
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_128544"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            215..>1071
                     /locus_tag="VEA63_04845"
     CDS             215..>1071
                     /locus_tag="VEA63_04845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HYD83350.1"
                     /translation="MFTRIDAPALSESQLYTALHPELSRWFKHRFAHFSPAQLHAVPE
                     ILAGQSLLLTSPTGSGKTLAAFLSVFDFLARAHDSGELPNGIVAVYVSPLRALAYDLR
                     KNLQQPAEELGWAWLRIGARTGDTTPKERAVQRRKPPDIFVTTPESLTLMLSQPAWIA
                     AFKSVRFFIADELHALAENKRGAMLMVAAERLEEVVGTPLQGGRETRREDRSKDGSES
                     RPDQNTRLTRIGLSATISPLETIAEFLVGPGRPCRIVEITQSKPAKIEVFSPLRDRAY
                     PPAGYTATRV"
BASE COUNT          193 a          404 c          286 g          188 t
ORIGIN      
        1 ccatcaaagc ggcttcgccc tgcgcttccc ccgcatcgcg cgcatccgcg aagacaaggc
       61 agtcgcagag atcgacacgc tcgcgacctg ccggcgcctc gccgccgcgg cgagcaacac
      121 tttcgcgtca tcctcttccg gaaggtagcg gcgctcgtcc cggcggccac cccatgagaa
      181 tggcggtcga gacgaccgcc gctaccattc aaaaatgttt acgcgcatcg atgctcccgc
      241 gctttccgag tcgcagctct acaccgcgct gcatccggaa ctctcgcgct ggttcaagca
      301 tcgcttcgcc cacttctccc cggcgcagct tcacgccgtc cctgaaattc tcgccggtca
      361 gtcgcttctt ctcacctcgc ccaccggcag cggcaaaacc ctcgcggcct ttctctcggt
      421 tttcgatttc ctcgcccgcg ctcacgattc tggcgaactc cccaatggta tcgtcgccgt
      481 ctacgtttcc cccttgcgcg cgctcgccta cgatctgcgc aaaaaccttc aacaacccgc
      541 tgaagagctc ggctgggcat ggctccgcat cggtgcccgc accggtgaca ccactcctaa
      601 agaacgcgcc gtgcagcgcc gcaagccgcc ggatattttc gttaccacgc cggaaagtct
      661 cacgttgatg ctcagccagc ccgcgtggat cgccgcattc aaatccgtcc ggttcttcat
      721 cgccgacgaa ctgcacgccc tcgccgaaaa caaacgcggc gcgatgctca tggtcgccgc
      781 cgaacgattg gaggaagtgg tcgggacgcc tctccaaggc ggccgcgaaa cgaggcgtga
      841 agatcgatcg aaagacggct cggagagccg gcccgaccag aacacccgcc tcacgcgcat
      901 cggtctctcc gcgacgatct caccgctcga aaccatcgcc gaatttctgg tcggccccgg
      961 ccggccgtgt cgcatcgtcg agatcacgca aagcaaaccg gcgaagatcg aggtcttctc
     1021 gccgctgcgc gaccgcgcgt atccgccggc cggctacacc gcgactcggg t
//
LOCUS       DASPLT010000469         1022 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_210720, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000469 DASPLT010000000
VERSION     DASPLT010000469.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1022)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1022)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1022
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_210720"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1022
                     /locus_tag="VEA63_04850"
     CDS             <1..>1022
                     /locus_tag="VEA63_04850"
                     /inference="COORDINATES: protein motif:HMM:NF024765.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate export family protein"
                     /protein_id="HYD83351.1"
                     /translation="TSYYFEAGSSSTQRESDPPSYVRNLGSAGNNDLAWLDLGLQQRV
                     RYEARDDDIRRFDAEGFDDPWLTRTRVFVGLRNRLDPLRLALEFQDSRRHSSRFASDN
                     RDVNKTEFIQAYAELYFADAFGVDPRGNQRPLSLRYGRHAFELLDRRLVGRNEWRNTT
                     NTFEGLRASIGQQSNDWQLEFVSLHPVTRLLDATDSADRNVRFNALIGHWRRSPRLAF
                     EPHYFHLRQDASAANGNRARDIHAPGLRVYGKSADGAINLDASLMKQFGNDGVQDHAA
                     WSVTAETGYTWLQHAWRPRLAAFYGYASGDRNPADGDSNRFERFFGFARPWSASDYVI
                     YENLHAP"
BASE COUNT          187 a          310 c          316 g          209 t
ORIGIN      
        1 acgtcctatt atttcgaagc cggctcttcg agcacgcagc gcgagagcga tccgccttcg
       61 tatgtgcgta atctcgggtc ggccggtaat aacgatctag cgtggctcga tctcggcctg
      121 cagcagcgtg tgcggtatga ggcgcgcgat gacgacattc gtcgcttcga tgcagaaggt
      181 ttcgacgatc cgtggctcac gcgtacgcgt gtgtttgtcg gcctccgcaa tcgcctcgat
      241 ccgttgcggc ttgcgcttga attccaggat tcgcggcgcc atagcagccg gttcgcgtct
      301 gacaatcgcg acgtcaacaa aaccgagttc attcaggctt atgcggaact gtatttcgcc
      361 gatgcgtttg gcgtcgatcc tcgtggcaac cagcggccgt tgagcctgcg ttacgggcgg
      421 catgcgttcg agctgctcga tcgtcgtctc gtcggccgca acgaatggcg caatacgacg
      481 aacacgttcg aagggctgcg cgcgagtatc ggccagcaaa gcaacgactg gcagctcgag
      541 ttcgtgagcc tgcatccggt cacgcgcctg ctcgacgcca ccgacagcgc cgaccgcaac
      601 gtgcgtttca atgcgctgat cgggcactgg cgccgctcac cgcgccttgc gttcgagccg
      661 cattattttc atctgcggca ggatgcgagc gcggcgaatg gcaatcgtgc gcgcgacatt
      721 catgcgccgg gcctgcgcgt ttatggcaag tcagcggacg gcgcgatcaa tctcgatgcc
      781 agcctgatga aacaattcgg caacgatggt gtgcaggatc acgcggcgtg gtcagtcacg
      841 gccgaaaccg gctatacgtg gctgcaacat gcatggcggc caagactggc tgcgttttac
      901 ggctacgcga gcggcgaccg caatcccgcc gatggagaca gcaaccgctt cgaacgtttc
      961 ttcggcttcg cgcggccgtg gtccgccagc gattatgtga tttacgaaaa cctgcacgcg
     1021 cc
//
LOCUS       DASPLT010000470         1331 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_251483, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000470 DASPLT010000000
VERSION     DASPLT010000470.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1331)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1331)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1331
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_251483"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..936
                     /locus_tag="VEA63_04855"
     CDS             <1..936
                     /locus_tag="VEA63_04855"
                     /inference="COORDINATES: protein motif:HMM:TIGR02603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD83352.1"
                     /translation="PADQAKAVWLSLARGYDGKDRSYLAALGIGATHKEAELYAALAA
                     TQTETDATKWTPAFADLAWKLTPADAAPDFAARATSEALTESERTAAVTALGFIPTNE
                     AANALVELAQNGVGQVKTHALWWILNYKDTRWKDAGLAGALKERGLYNPDTAAVVPSV
                     VPEPSPTKLATPVQIAALTGDATRGANIGQSCYLCHRIGDKGIDYAPALTGFASRQTA
                     EVLITAIVNPSADIAHGYSGSEVVLKDGTTVHGLVLSSGDPLIVQSMGGLTQLIPQER
                     VASRKPLRRSLMLSAEQLGLSAQDVADVAAWLATQ"
     gene            1075..>1331
                     /locus_tag="VEA63_04860"
     CDS             1075..>1331
                     /locus_tag="VEA63_04860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD83353.1"
                     /translation="MTPLSPVRATEPVPSFSARQMATVDLLWTGGWDSTYRLLDLVLR
                     KSRTVAPHYVVDPDRSGTIQELRAMRAIRQEAAKSDRAAADR"
BASE COUNT          235 a          375 c          491 g          230 t
ORIGIN      
        1 ccggcggacc aggcgaaggc ggtttggttg tcgttggcgc gcggatacga cggcaaggat
       61 cgctcctatc tcgcggcgct cggcattggc gcgacgcata aggaagcgga gctgtatgcc
      121 gcgctggcgg cgacgcagac ggagacggat gcgacgaagt ggacgccggc gtttgccgat
      181 ctcgcgtgga agctcacgcc tgcagacgca gcgccggact tcgccgcgcg agcgacgtcg
      241 gaagcgttga cggagagcga gcggacggcg gcggtgacgg cgctcggttt tattccgacg
      301 aacgaagcgg cgaacgcatt ggtcgaactc gcgcagaacg gcgtcggcca ggtgaagacc
      361 cacgcgctgt ggtggattct caactacaag gatacgcgct ggaaagacgc cgggctcgct
      421 ggcgcgttga aagagcgtgg tctctataat ccggatacgg cggccgtggt gccaagcgtc
      481 gtgcccgagc cgtcgccgac gaagctggcg acgccagtgc aaatcgcggc gttgacgggc
      541 gacgcgacac gcggtgcgaa catcgggcag agttgctatt tgtgtcaccg catcggcgac
      601 aaaggcatcg attacgcgcc ggcgttgacg ggtttcgcga gccggcaaac cgcggaggtg
      661 ttgatcaccg cgatcgtgaa tccgtcggcg gatatcgcgc atggttatag cggcagcgag
      721 gtcgtgttga aggacggcac gacggtgcac gggctggtgc tttcgagcgg cgatccgctc
      781 atcgtgcaat cgatgggtgg gttgacgcag ttgatcccgc aagagcgggt cgcgtcgcgc
      841 aagccgctgc ggcgatcgtt gatgttgagc gcggaacaac ttgggttgag cgcgcaggac
      901 gtggcggacg tcgcggcgtg gctggcgacg caatagcgcc ggagcttttc cgtcgggcgc
      961 gcgatggcgt gccttgtgtc agccgggcgc gcacgcggtc atcggagatg cacccggctg
     1021 gctgatgagg gaagcagtcg tttgcaaaaa cggttgctgg ttgcttggtg atgaatgacc
     1081 ccgctctccc cagttcgcgc caccgaaccc gttccctcat tttcggcccg gcagatggcg
     1141 acggttgatc ttctctggac gggaggatgg gactcgacgt atcggctgct cgatctggtg
     1201 ctgcgcaaga gccgcactgt cgcgcctcat tacgtggtgg atccggatcg cagcggcacg
     1261 attcaagaac tccgggcgat gcgcgcgatc cggcaggaag cggcgaaaag cgatcgcgcg
     1321 gcggccgatc g
//
LOCUS       DASPLT010000471         1349 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_128627, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000471 DASPLT010000000
VERSION     DASPLT010000471.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1349)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1349)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1349
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_128627"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..444
                     /locus_tag="VEA63_04865"
     CDS             <1..444
                     /locus_tag="VEA63_04865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD83354.1"
                     /translation="TTTMTNLQEITTKVNQGEGTLGRLVNDPALYNELLTTVADIKNA
                     AAGAKDFMANAQGIIDQVKSGQGTLGALIYDNTTGDNIKATVANFRSVSDKIAKGEGT
                     LGKLINDDQLFQSAQSTLKKADRALDGMSDSGPITAVGIVANSLF"
     gene            complement(583..>1349)
                     /locus_tag="VEA63_04870"
     CDS             complement(583..>1349)
                     /locus_tag="VEA63_04870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD83355.1"
                     /translation="AYEVADRTLTDWIATERRKSGRKAPPAAERRGAPLIAGFAPLVA
                     KFSRSIAEDHPFAPMLMRRPIAILCALSLVGLGLLAASSKAPAVSSAPTEARQFDFWL
                     GEWSFASADGRLQGSSKVELVADGYGLLEQRLSETTEGNVSGFSLNAWNAEKKQWQQF
                     WIGESGALLELAGGLDAGGRMVLQGEQHADGERTLNRITWAPGAVGTVRQHWEQSRDG
                     GRTWATVFDGMYARKVAKLGGGAGGGHSRAMYYMFL"
BASE COUNT          277 a          493 c          360 g          219 t
ORIGIN      
        1 acgaccacga tgacgaacct ccaggaaatc accaccaagg tgaatcaagg cgaaggcacc
       61 ctcggccgcc tcgtcaacga tcccgcgctc tacaacgagt tgctcaccac cgtcgccgat
      121 atcaagaacg ccgcggctgg cgcgaaagat ttcatggcga atgcgcaggg catcatcgac
      181 caggtgaaga gcggccaggg cacgctcggc gcgttgatct acgacaacac gaccggcgac
      241 aacatcaagg caaccgtcgc caacttccgc agcgtgtccg acaaaatcgc gaagggcgaa
      301 ggcacgctcg gcaagttgat caacgacgat caactcttcc aaagcgcgca gtcgaccttg
      361 aagaaagccg atcgcgcact cgatggcatg agcgattccg gcccgatcac cgccgtcggc
      421 atcgtcgcaa actcactctt ctgatccgcc gcatcgcacg gagtccgtag cggcaggcct
      481 ccgtgcctgc ctgcagcaac cgcccgacaa accgtcgcgc tccaatcgga ctacaacgct
      541 cctctcaccc ttcgcatcgg cgcaccgctc acgcgccacc cgtcaaagaa acatgtaata
      601 catcgcgcgc gaatgtcctc cgcctgcgcc gccgccgagc ttcgcaacct tccgcgcata
      661 catcccatcg aagactgtcg cccacgtgcg gccgccatcg cgcgactgct cccaatgttg
      721 gcgcaccgtc cccacagcgc ccggcgccca cgtgatccgg ttgagcgtgc gttcgccgtc
      781 agcgtgttgt tcgccctgaa gcaccattcg cccgccggcg tcgagcccgc ccgccaactc
      841 gagcaatgct ccgctctcgc cgatccaaaa ttgctgccac tgcttcttct cggcgttcca
      901 cgcgttcaaa ctgaacccgc tcacgttccc ctctgtcgtt tcgctcaagc gctgttcgag
      961 caagccatag ccatccgcca ccaactcgac cttgctcgat ccttgcagcc ggccatcggc
     1021 tgaggcgaaa ctccattcgc cgagccagaa atcgaactgc cgcgcttccg tcggtgccga
     1081 agaaaccgcc ggcgccttcg acgaggccgc caggagcccg agtccaacca ggctcagcgc
     1141 acacagaatt gcgatgggac gtcgcatcag catcggcgca aaaggatgat cttctgcgat
     1201 ggagcgcgag aactttgcga cgagcggcgc aaagcctgcg atcagcggcg cccctcgacg
     1261 ctcggctgcg ggaggggctt tacgccccga cttgcgccgc tcagttgcta tccagtcggt
     1321 aagcgtccgg tccgcgacct cataggcgg
//
LOCUS       DASPLT010000472         1276 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_5992, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000472 DASPLT010000000
VERSION     DASPLT010000472.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1276)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1276)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1276
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5992"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..271)
                     /locus_tag="VEA63_04875"
     CDS             complement(<1..271)
                     /locus_tag="VEA63_04875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009514032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HYD83356.1"
                     /translation="MAKTLDVQPLRRPALEANFLPAALWTRAYRALVASDRGVRRFAL
                     ALTRADGSASRYETRVLGAEHPAAHLNFKYVERLLKFLLWQKGGWR"
     gene            complement(284..>1276)
                     /locus_tag="VEA63_04880"
     CDS             complement(284..>1276)
                     /locus_tag="VEA63_04880"
                     /inference="COORDINATES: protein motif:HMM:NF014625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HYD83357.1"
                     /translation="MADLLTEHGQGYGLNIRIFNELQHTITGWPGGKPDADDSARPER
                     AAPFPKRVVVFSPEPSHDVLGMGGTLRRLQEQGHAVTVIYLTSGNLALPDEEAAMAVD
                     FIGELGEGLELAPGAVRNFVDEVREQLTAKKAFASDPPPVRRLKGLLRRSEARASLRD
                     CGIRPAQVRFLDLGFYERGRYRQFAPDAADLAAIVRALREAAPNQIFVTGDRDDPSSV
                     SAVCFELVRRAWRELATEAWRTDCRVWLYRGVEAPWAAAEIDMAVPLSPRELAQKTQA
                     VFQHRSQRGQTPVATGLREPWQQAEQQNRGLAKIYDQLGLADYEAIEGFRRASL"
BASE COUNT          235 a          478 c          353 g          210 t
ORIGIN      
        1 cccgccagcc gcctttctgc cagagcagaa acttcagcaa tcgctccacg tatttgaaat
       61 tcaaatgcgc tgctggatgc tcggcgccga gcacgcgggt ctcgtatcgc gacgcacttc
      121 catcggcgcg cgtcaacgcc agcgcgaacc ttcgcacacc gcgatcgctg gcgacgagcg
      181 cgcgataagc gcgcgtccac aacgccgccg gcagaaagtt cgcttccagc gccgggcgcc
      241 gcagcggttg aacatccagc gtctttgcca ttttgagcta ttttcagagc gacgcgcgcc
      301 ggaaaccctc gatcgcttcg tagtccgcga ggccaagctg atcgtaaatt ttcgcgagcc
      361 cccgattctg ctgctcggcc tgctgccacg gttcgcgtaa acccgtcgcc accggcgtct
      421 ggccgcgctg gctgcgatgt tgaaacaccg cctgcgtttt ctgcgcgagt tcgcgcgggc
      481 tgagcggcac cgccatgtcg atctccgccg ccgcccacgg cgcttcaacc ccacgataca
      541 accacacgcg gcagtccgtg cgccacgctt ccgtcgcgag ctcccgccag gcccggcgaa
      601 cgagttcgaa acacaccgcg gacaccgacg agggatcgtc gcgatcgccc gtcacaaaaa
      661 tctgattcgg cgcggcctcg cgcagcgccc gcacgatcgc cgcgagatcg gccgcgtccg
      721 gcgcaaactg gcgataacgc ccgcgctcat aaaaaccgag atcgagaaac cgcacctgcg
      781 ccggacgaat tccgcaatcg cgcaacgacg cccgcgcctc gctgcggcgc aacaatcctt
      841 tcaaccggcg caccggcggc ggatcgctcg cgaacgcctt ctttgccgtc aattgctcac
      901 gcacttcatc gacaaaattc cgcaccgcgc caggcgcgag ttccagtcct tcgccgagct
      961 cgccgatgaa atccaccgcc atcgccgcct cttcgtccgg cagcgcaaga ttgcccgacg
     1021 tcaaataaat caccgtcacc gcgtgaccct gctcctgcag ccgccgcaac gtgccgccca
     1081 tgccgagcac gtcgtgcgac ggctccgggc tgaaaacgac gacgcgcttc ggaaacggcg
     1141 ccgcgcgctc cggccgcgcc gaatcgtccg cgtccggttt cccgcccggc cagccggtga
     1201 tcgtatgttg aagctcgtta aaaatccgga tgttcaaccc gtagccctgg ccgtgctccg
     1261 tcaatagatc cgccat
//
LOCUS       DASPLT010000473         1681 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_28931, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000473 DASPLT010000000
VERSION     DASPLT010000473.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1681)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1681)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1681
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_28931"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1681
                     /locus_tag="VEA63_04885"
     CDS             <1..>1681
                     /locus_tag="VEA63_04885"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HYD83358.1"
                     /translation="PDCEIFLVATKADYEARLNRADFDLILSDFSLVQFDGSEALQLA
                     RKHVPDIPFIFLSGNIGEDRAIEAVKAGAQDYVLKDRVKRLTTAIDRALRESADRRRV
                     RAAEADRAHLAATLENSPDFVGLASPDGHILYLNRAAREMLRLPEGTDIRLLRLEDVH
                     PPEVLNRLTQDGLVTAARDGTWTGESIVLARDGHTTPVSQVIVAHREADRTLGYYSTV
                     MRDLTARKRTEALVNGQNQILEMIAGSEPLGETLTALLRFIEGQCDEMLCSILVLEED
                     GRRIRHRAAPRLPLGYIQAVDNTPIGPEVGSCGAAIHRRAPVIVTDIATDPLWAEHRD
                     LTLGYGLRACWSTPIYDGHHRILGTFAAYLDHPGAPNEQHRQLIDIGVHIAAICLSRH
                     HSERRLRDQANILDKASDAIAITDHNHRILFWNHSAERTLGWTAAEARGREYGEIFGA
                     AAIAQIEAAGKATDERSEWQGEVHLKNRQGQSVIMECRVTVLRDEAGEPQGRLVIATD
                     VTARKRIEEQFFRAQRLESIGMLAAGIAHDLNNVLAPILLAAPMLRDHASDP"
BASE COUNT          318 a          623 c          471 g          269 t
ORIGIN      
        1 gcccgactgt gagatctttc tcgtcgcgac caaggccgac tacgaggccc ggctcaaccg
       61 cgccgatttc gatctcatcc tctcggattt ctccctcgtg cagttcgacg gcagcgaagc
      121 gctgcagctc gcgcgcaaac acgtgcccga tatcccgttt atttttctct ccggcaacat
      181 cggtgaagac cgcgcgatcg aagccgtgaa agccggcgcg caggactacg tgctgaaaga
      241 tcgcgtcaaa cgcctcacca ccgcgatcga tcgagcgctc cgcgaaagcg ccgatcgccg
      301 gcgtgtccgc gccgccgaag ccgatcgcgc gcacctcgcc gcaacgctcg aaaacagtcc
      361 cgatttcgtc ggcctcgcct cccccgacgg ccacattctc taccttaacc gcgctgcgcg
      421 cgagatgctc cggttgccgg agggcacgga catccggctc cttcgcctcg aggacgttca
      481 cccgcccgaa gtcttgaacc gcttgactca ggacggcctc gtcacggccg ctcgcgatgg
      541 cacctggacc ggcgagagca tcgtgctcgc gcgagatggc cacacgacgc cggtctcaca
      601 ggtgatcgtc gctcatcgcg aagccgatcg cacgctcggc tattactcga ccgtcatgcg
      661 cgacctcacc gcgcgcaagc gcaccgaagc gctcgtcaac ggccagaatc aaattctcga
      721 aatgatcgcc ggcagcgagc cgctcggcga aacactcact gcgctcctgc ggttcatcga
      781 aggccagtgc gacgagatgc tctgctccat tttggtgctc gaggaagacg gtcgacgcat
      841 ccgtcaccgc gccgcgccgc gactgccgct cggctacatt caggcggtcg acaacacccc
      901 gatcggcccc gaagtcggct cctgcggcgc cgcgatccac cgccgcgcgc ccgtgatcgt
      961 caccgatatc gcgaccgatc cgctctgggc ggaacatcgc gatctcacgc tcggctacgg
     1021 cctgcgcgcg tgttggtcga cgcccattta cgacggccat catcgcatcc tcggcacttt
     1081 cgcagcctac ctcgatcatc ccggtgcgcc caacgaacag caccggcagt tgatcgacat
     1141 cggcgtccat atcgccgcca tctgcctcag ccggcatcat tccgaacgcc ggttgcgcga
     1201 tcaggccaac atcctcgaca aagcgagcga cgcgatcgcg atcaccgatc acaaccaccg
     1261 catcctcttc tggaatcaca gcgccgaacg cacgctcggc tggaccgcgg ccgaagcgcg
     1321 cggccgcgaa tacggcgaga ttttcggtgc cgcagccatc gctcagatcg aagccgccgg
     1381 caaagcgacc gacgaacgct cggagtggca gggcgaagtt cacctgaaaa accgccaggg
     1441 ccaatccgtg atcatggaat gccgcgtcac cgttttgcgc gacgaggccg gcgaaccgca
     1501 agggcgcctc gtgatcgcca ccgacgtcac cgcgcgaaaa cgcatcgaag aacagttctt
     1561 ccgtgcccag cggctcgaaa gcatcggcat gctcgccgcc ggcatcgcgc acgacctcaa
     1621 caacgttctc gcgccgatcc ttctcgccgc gccgatgctg cgagaccacg cctccgaccc
     1681 g
//
LOCUS       DASPLT010000474         1097 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_128693, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000474 DASPLT010000000
VERSION     DASPLT010000474.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1097)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1097)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1097
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_128693"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            142..>1097
                     /locus_tag="VEA63_04890"
     CDS             142..>1097
                     /locus_tag="VEA63_04890"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ectonucleotide
                     pyrophosphatase/phosphodiesterase"
                     /protein_id="HYD83359.1"
                     /translation="MKPTQCVILALAVAAMLVRAEVAPQRSLLLISIDGLRPDYISEA
                     DRHGLKVPHLREIWENGAHAERVRGVLPTSTYPSHTAIITGTSPARHGIGSNQPFDPT
                     DSRPYRWYWYTEDLKTPALWDAASAAGYEVGSVSWPVTVGAKGIKYNLADFTGTRSDE
                     DAKMIRAWAGPGFMDALAEKAGVFLTDSKLGTKRDRARARYIVEIIRQKKPRLMLTHF
                     VGTDHYQHQMGPFTAATYVAIQEVDEMVGEITAAMRREYPDAAVCVVSDHGFSRVDRS
                     LLITAAFVRAGLVTLEKNCASVAEAGLKDWIAMPWLAGGSAA"
BASE COUNT          210 a          297 c          368 g          222 t
ORIGIN      
        1 cttcaattcc ttcgttggcg aagtcatgca aaccgtgggg gccacttgga ttttcgtgat
       61 catggcgttc ctgcatccgc tcgcgtcgct tatcctgtgg acgatggtgc gaccggaaaa
      121 accgtcggag actgcggctg catgaaaccg acgcagtgcg tgattctcgc tcttgccgtt
      181 gcggcgatgt tggtccgtgc ggaagtggcg ccgcagcgca gcttgctgtt gatctcgatc
      241 gacggcttgc gcccggacta catcagcgaa gcggatcgtc acggactgaa ggttccgcat
      301 ttacgcgaaa tttgggaaaa cggcgcgcac gccgaacgtg tgcgcggtgt gttgccgacg
      361 tcgacgtatc cgagtcacac cgcgatcatc accggcacat caccggcgcg tcatggcatc
      421 ggatccaatc agccgttcga tccgactgac tcgcggccgt atcgctggta ttggtatacc
      481 gaggacctga agacgccggc gttgtgggat gcggcgagtg cagccggcta cgaagtgggc
      541 agcgtgagct ggccggtgac ggtcggcgcg aagggaatca aatataatct ggccgatttt
      601 accggcacgc gcagcgacga ggatgcgaag atgattcgcg cgtgggcagg gcccgggttc
      661 atggacgcac tcgccgagaa agcgggagtg tttctcaccg actcaaaact cggcaccaaa
      721 cgcgatcgcg cgcgtgcgcg ttacatcgtc gaaatcattc gtcagaaaaa accgcggctg
      781 atgctgacgc atttcgtcgg cacggaccac tatcaacacc agatgggtcc gtttaccgcg
      841 gcgacgtatg tggcgatcca ggaggtcgac gaaatggtgg gtgaaatcac ggcggccatg
      901 cggcgcgagt atccggatgc ggcggtgtgt gtggtgtcgg atcacgggtt ttcccgcgtt
      961 gatcgctcgc ttctgataac ggcggcattt gtgcgggcgg gactcgtcac gctcgaaaag
     1021 aactgcgcga gtgtggccga ggcaggtctg aaggattgga ttgcgatgcc gtggctcgcg
     1081 ggcggttcgg cggcaat
//
LOCUS       DASPLT010000475         1992 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_190761, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000475 DASPLT010000000
VERSION     DASPLT010000475.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1992)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1992)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1992
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_190761"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(246..1667)
                     /locus_tag="VEA63_04895"
     CDS             complement(246..1667)
                     /locus_tag="VEA63_04895"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HYD83360.1"
                     /translation="MAFSSPALLRKVVLPSLLLAGDTVVAFASLSIGYWLRYVTPVGA
                     IGIEVPDARYADYLPLLLVGTAFLVAAYAHLDLYEERFLLRKFQSLNLIIKGTTFWLA
                     AYLSLSVVLYFQPPISRWFVVLSYFVTLTLMFVWRTAAYALFTRTRLVSRLRQRVAVL
                     GWNNDAATLVADLAAMSAHPFRFCGHIALPDDSPPADIPQLGPADHLRALLRGHQIDV
                     LIAARTDLPRDQLQSIVEVCEQTFVEWKVVPSSFQILVSGLRLQTVGRIPVLGVESLA
                     IDRLFNRTLKQLLDLIGGFIGLILSAPLIAFFAVLIKRESPAGPVFFAQTRIGAGGRP
                     FTLWKLRSMQPDAAATDHLAVSTARGDARLLRIGAFLRRWNFDELPQFWNVLRGDLSL
                     VGPRPERPHHVHRLAGEIPHYLPRHLVKPGLTGWAQINGLRGDTSIPRRIQHDIYYIE
                     NWSLWLDLQILALTFFRWRNRAE"
     gene            complement(1677..>1992)
                     /locus_tag="VEA63_04900"
     CDS             complement(1677..>1992)
                     /locus_tag="VEA63_04900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD83361.1"
                     /translation="PLTPLRPEILRLAASIPLPNTPAANALRLAWSSPPLSAPFPNGP
                     AFRRVRTGYGVLMSHPDGPPPIDVNIQASPQLPPDLAARLPNKGWLRGDFLLEFLASS
                     PR"
BASE COUNT          399 a          440 c          852 g          301 t
ORIGIN      
        1 gatgattcgt tgaaccacca aggggcacga atgaacacga agggaaaatg tggggttcaa
       61 ttccggagtg gtgaaccacg gatggacacg gatcgaaacg gcggagcgga ggcgcgaggg
      121 aagttgtggg gcttgggctg attcgaccgc aactcgacta gtttctgtgg acgggaggat
      181 tttctggttt tcgatggcct tgcgcggacg cctcggagag gcgtccctac cggaattcgc
      241 gcgggtcact cggcgcggtt gcgccagcgg aagaaggtga gggcgaggat ctgcaggtcg
      301 agccagaggg accaattttc gatgtagtaa atatcgtgct ggatgcggcg cggaatgctg
      361 gtgtcgccac ggaggccgtt gatctgggcc cagccggtga ggccgggttt cacgagatga
      421 cgggggagat aatgcgggat ttcgccggcg agacggtgga cgtgatgcgg gcgttcgggg
      481 cgcggaccga cgaggctgag gtcgccgcgg aggacgttcc agaattgggg aagttcgtcg
      541 aagttccagc ggcgaagaaa agcgccgatg cggaggaggc gggcgtcgcc gcgcgcggtg
      601 ctgacggcga ggtgatcggt cgcggcggcg tcgggttgca tgctgcggag cttccagagc
      661 gtgaacgggc ggccgccggc gccgatgcgg gtttgcgcga aaaagacagg gccggcgggc
      721 gactcgcgtt tgatgaggac ggcgaagaac gcgatgagcg gcgcggagag aatcaggccg
      781 atgaagccgc cgatgagatc gaggagttgt ttgagcgtgc ggttgaagag gcggtcgata
      841 gcgagcgatt cgacaccgag cacggggatg cggccgacgg tttggaggcg gaggccgctg
      901 acgagaattt ggaaggagga gggaacgacc ttccactcga cgaaggtttg ttcgcagacc
      961 tcgacgattg actggagttg gtcgcgcgga agatcggtgc gcgcggcgat gagcacgtcg
     1021 atttgatggc cgcgcaggag agcgcggaga tggtcggcgg ggccgagttg cgggatgtcg
     1081 gcgggaggag aatcgtcggg cagcgcgatg tgtccgcaga agcgaaatgg atgggcggac
     1141 atcgcggcga gatcggcgac gagcgtggcg gcgtcgttgt tccagccgag gacggcgacg
     1201 cgctggcgga ggcgggagac gaggcgcgtg cgggtgaaga gcgcgtaggc ggcggtgcgc
     1261 cagacgaaca tgagcgtgag cgtgacgaag taggagagga cgacgaacca gcgggagatc
     1321 ggaggttgaa agtagaggac gacggagagg ctgaggtagg cggcgagcca gaatgtggtt
     1381 cccttgatga tgaggttgag cgactggaat tttcgaagga ggaaacgttc ctcgtagaga
     1441 tcgaggtgcg cgtaggcggc gacgagaaag gcggtgccga cgagcaggag cggaagatag
     1501 tcggcgtaac gggcgtcggg gacctcgatg ccgatcgcgc cgacgggtgt gacgtagcgc
     1561 agccagtagc cgatcgagag gctcgcgaag gcgacgacag tgtcgccggc gaggaggagc
     1621 gaaggcagga cgactttgcg caggagcgct gggctggaga acgccatgat gcgtgtttag
     1681 cgcggggagg aggcgaggaa ttcgagcagg aaatcaccgc gaagccatcc tttgttggga
     1741 aggcgcgcgg cgaggtcggg cgggagttgg ggcgaggctt ggatgttgac gtcgatgggc
     1801 ggcgggccgt cggggtggct catcaatacg ccgtagccgg tgcgcacgcg gcggaaggcg
     1861 gggccgttcg gaaacggagc ggataaaggc gggctggacc aagcgaggcg aagggcgttt
     1921 gcggcggggg tgttcgggag cgggatggat gcggcgaggc gaaggatttc ggggcgcaag
     1981 ggagtgagcg gg
//
LOCUS       DASPLT010000476         1150 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_6033, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000476 DASPLT010000000
VERSION     DASPLT010000476.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1150)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1150)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1150
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6033"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..790)
                     /locus_tag="VEA63_04905"
     CDS             complement(<1..790)
                     /locus_tag="VEA63_04905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD83362.1"
                     /translation="MTNSLTKLLKAGPPPPQVVLLPDEVFFTRAIAVPAGATAIETAS
                     LVELALETLSPFPPAQLYHGYYWPSGAERALVFATYRRRFTVEQTAEWQVAELVLPAF
                     ATLLGGDVQAHTTLIVPSPEGVTAIYWDDQPVPARVTYRPVPPDSAETEAAKVREELL
                     AVMPRNHAYVLKAAPVADSSGSDSEIVFRSDDFVSQLPKTRTAAMDVRDKEALTALRR
                     AQSRDVLLWRTFLGFAAILLLLALGELALIGASFWQHTRVARANQ"
     gene            complement(787..>1150)
                     /locus_tag="VEA63_04910"
     CDS             complement(787..>1150)
                     /locus_tag="VEA63_04910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD83363.1"
                     /translation="FFRSTEEASALLGSGGLPQSAGTEISALRITITIREGRTEFRLS
                     AVVAPSGGAKAVDAIAQPAEHRTLASTNEQPEESPPPETNETGSGSAKKLKYPFTLLE
                     IRENDEISSAPAVADTQA"
BASE COUNT          205 a          382 c          363 g          200 t
ORIGIN      
        1 gttggttggc gcgtgcgacg cgggtgtgct gccagaaact cgcgccgatc agcgccagct
       61 cgccgagagc gagcagcagg aggatcgccg cgaacccgag gaaagtccgc cagagcagca
      121 cgtcgcgact ctgcgcccgg cggagcgctg tgagcgcttc cttgtcgcgc acgtccatcg
      181 cggcggtgcg ggttttcggc agttgcgaga cgaagtcgtc gcttcgaaac acgatctcgc
      241 tgtcgctgcc ggatgagtca gcaaccggtg cggccttcag cacgtaggcg tgattgcgcg
      301 gcatcaccgc cagcagttct tcgcgcacct tggctgcctc ggtctctgcc gagtcaggcg
      361 gcaccgggcg ataagtgact ctggctggca cgggctgatc atcccaataa atcgcggtca
      421 cgccttcggg cgacggcacg atgagcgtcg tgtgcgcttg cacatcacca cccagcagag
      481 tggcaaacgc cgggagcacg agctccgcga cctgccactc cgcggtttgc tcaaccgtga
      541 accgccgacg ataggtcgcg aaaacgagcg cgcgctccgc cccagacggc cagtaatagc
      601 cgtggtagag ctgcgccggc ggaaagggcg aaagcgtctc caacgcgagc tcgacgaggc
      661 tcgccgtctc gatcgcggtg gcaccggcag gcacggcgat cgcccgcgta aaaaacacct
      721 catcgggcag gagcacgact tgcggcggcg gcggcccggc tttcaacaat ttggtcagcg
      781 agttcgtcat gcctgtgtgt ccgcgactgc aggagccgag gaaatctcat cattctcccg
      841 aatttccaag agcgtaaatg gatacttcaa ttttttcgcc gagccgctcc ccgtttcatt
      901 ggtctcaggc ggaggcgatt cctccggttg ctcgtttgtc gacgccagcg tgcgatgctc
      961 cgccggctgt gcgatcgcat caacggcttt ggcgccaccc gacggcgcga cgaccgccga
     1021 cagccgaaac tccgtcctgc cctcgcgaat cgtgatggtg atgcgcaatg cgctgatctc
     1081 cgtccccgcc gattgcggca gcccgccgct gccaagcaac gcactcgcct cctccgtgct
     1141 gcggaaaaac
//
LOCUS       DASPLT010000477         1117 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_128769, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000477 DASPLT010000000
VERSION     DASPLT010000477.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1117)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1117)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1117
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_128769"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..605)
                     /locus_tag="VEA63_04915"
     CDS             complement(<1..605)
                     /locus_tag="VEA63_04915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012372863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HYD83364.1"
                     /translation="MAIRVGISGWTYAPWRGSFFPKGLAQKRELAFAAAHVGSIEING
                     TFYSMQRPACYASWYEQTPGDFLFAVKGPRFITHLKRLKDVVAPTANFFASGVLRLKE
                     KLGPILWQLPARTAFDPERLTEFFNLLPRSTREAAVLGRLHDHRLKARAWLRVDEDRP
                     IRHALEVRHPSFENEEFIALLRKHDIACVVADTAGKWPRLH"
     gene            873..1049
                     /locus_tag="VEA63_04920"
     CDS             873..1049
                     /locus_tag="VEA63_04920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD83365.1"
                     /translation="MGFDKDDTRPVVDVEKRTTKVNISMVIGVIVFFIVTGIVVWALF
                     IRPSAGGETGVPLP"
BASE COUNT          209 a          385 c          320 g          203 t
ORIGIN      
        1 tcgtgcagcc gcggccactt ccccgcggtg tccgccacca cgcacgcgat gtcgtgcttc
       61 cgcagcaacg cgatgaattc ctcgttctcg aacgacggat gccgcacttc gagcgcgtgc
      121 cggatcggcc ggtcctcatc cacgcgcaac cacgcgcgcg ctttcaaacg gtgatcgtgc
      181 agccgcccga ggaccgccgc ttcgcgcgtg gaacgcggca gcaggttgaa aaactccgtc
      241 aggcgctccg gatcgaacgc cgtgcgcgcc ggcaattgcc acaggatcgg cccgagtttc
      301 tctttcaacc gcagcacacc cgacgcgaaa aaattcgccg tcggcgccac gacgtccttc
      361 agacgcttga gatgcgtgat gaaccgcgga cctttcaccg cgaacaggaa atcccccggc
      421 gtctgctcat accacgacgc gtagcacgcg ggccgctgca tcgaatagaa cgtgccgttg
      481 atttcgatcg agcccacgtg cgccgccgca aacgcgagct cacgtttttg cgcgagacct
      541 ttcggaaaaa aactcccgcg ccacggcgcg taggtccaac cggagatgcc aacgcgaata
      601 gccatcgatc aacctagcct caccccggcg cgcaagccat cggctaaatc ggggcgcgct
      661 gcatgggatc atctccgaat tcgaggcctt cggcattagg ctgggaactc gtttccgcac
      721 ttggtgagcc tgtgcgctca ccgtcgcgcc gcgtggcacc gtgggcggtt tggactgtcg
      781 gtgaactcat tcaatcctca tccgcgtcgc gcgtcgttgc ccgcatccgc cgccgccgcg
      841 atcccccccc gcaccctcaa tcgctaaatc ttatgggctt cgataaagac gacactcgtc
      901 ccgtggtgga cgtggaaaaa cgcaccacga aggtcaacat ctcgatggtg atcggcgtca
      961 tcgttttctt catcgtgacc ggaattgtgg tctgggcgct cttcattcgt ccgtccgccg
     1021 gcggcgaaac gggcgtgccg ctcccgtaaa aaaaaagacc gtccgcgtcg cggcgcgttt
     1081 cgggatttcc ccgggactga aacggcggcg tcacgcg
//
LOCUS       DASPLT010000478         1195 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_6082, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000478 DASPLT010000000
VERSION     DASPLT010000478.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1195)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1195)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1195
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6082"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..536
                     /locus_tag="VEA63_04925"
     CDS             <1..536
                     /locus_tag="VEA63_04925"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HYD83366.1"
                     /translation="PAAEKMFREALVIRRNGLGPSHPALATTLNNLAHALREQARFDE
                     AQAAVVEAIEITRAALGDEHPRIAAYTAHLAQIYLERGDAAHAEPLARAALVLRQRVS
                     PGDAWRIAATQSLLGAALAALARYDEAEPLLLEADRVLQDIPGPQGRETAAHRARLAA
                     FRETRRLAASETSESDR"
     gene            complement(1002..>1195)
                     /locus_tag="VEA63_04930"
     CDS             complement(1002..>1195)
                     /locus_tag="VEA63_04930"
                     /inference="COORDINATES: protein motif:HMM:NF012900.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HYD83367.1"
                     /translation="REIVTRYAGVPVILAGGLNPENVAAAVRQVRPAGVDANSGTKGA
                     DGFKDAARVRAFVRAAKEA"
BASE COUNT          220 a          473 c          301 g          201 t
ORIGIN      
        1 gccccgccgc cgaaaaaatg ttccgcgaag cgctggtcat tcgccgcaac ggcctcggcc
       61 cgagccatcc tgccctcgcc accacgctga acaacctcgc ccacgcgctg cgcgaacaag
      121 cgcgattcga cgaagcccag gccgccgtcg tcgaagcgat cgagatcacc cgcgccgcgc
      181 tcggcgacga acatccgcgt atcgccgcct acaccgcgca tctcgcgcag atttacctcg
      241 aacgcggcga cgccgcccac gccgaaccgc tcgcacgcgc cgcgctggtg cttcgccaac
      301 gcgtgtcgcc cggcgacgcc tggcgcatcg ccgccacgca aagcctgctc ggcgccgccc
      361 tcgccgccct cgcccgttac gacgaggccg agccgttgct cctcgaggcc gaccgcgttc
      421 tgcaggatat tcccggtccg caaggccgtg aaaccgccgc tcatcgcgcc cgcctcgccg
      481 cctttcgcga aacccgccgt cttgccgcgt cggaaacctc cgaaagcgat cgctgaactc
      541 ctctgccgct tcgcccgcaa accccgcttc gccgatgtct ctaacgaggc tttgccccag
      601 cccgacgaga tatccgaaaa gcaatacagt taaccgctca ttatcgctcg tcttccccca
      661 attccgacga aacgtccgcg gttcgccgat taggcgaagc acgcactcgt tcgccctcac
      721 caccggtctc agaaaaatca gcgcagatta gcggttcaac agctcgtcct aatgaattcg
      781 gaatcgaacg gtagctaacc gctaattgac gctgatcttc gctaatggaa tccgggcagt
      841 cccaccaaac gtccgcgctt cgccgattag gcgaagcacg cactcgttcg ccctcacgag
      901 cggtctctga aaatcagcgc agattagcgg ttcaagaact ctgccttccg tagtcaaact
      961 ttgccccggc aaggcctgca gctcttccgt cctctgtttt ctcacgcctc cttggccgcg
     1021 cgcacgaacg cccgcacgcg cgccgcatcc ttgaacccgt ccgcgccttt cgtgccggag
     1081 ttcgcatcga cacccgccgg ccgcacctgc cgcaccgccg ccgccacgtt ttccgggttc
     1141 agtccgcccg cgagaatcac cggcacaccc gcataacgcg taacaatctc gcgac
//
LOCUS       DASPLT010000479         1103 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_6097, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000479 DASPLT010000000
VERSION     DASPLT010000479.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1103)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1103)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1103
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6097"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..249
                     /locus_tag="VEA63_04935"
     CDS             <1..249
                     /locus_tag="VEA63_04935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD83368.1"
                     /translation="SLLAVIGAGLAMAAWRERLTAEWIFLAAAAPLVLAIVDIVFVTR
                     DVIPPIYLADAAGELALAALWCVIAIARVQNNRAGKTR"
     gene            345..>1103
                     /locus_tag="VEA63_04940"
     CDS             345..>1103
                     /locus_tag="VEA63_04940"
                     /inference="COORDINATES: protein motif:HMM:TIGR03916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA modification/repair radical SAM
                     protein"
                     /protein_id="HYD83369.1"
                     /translation="MDLQQKLSILADAAKYDASCASSGTTLRNSRESGGIGSTTATGI
                     CHSYTPDGRCVSLLKILLTNYCTYDCLYCVNRISSSVPRARFTPDEVVNLTLDFYRRN
                     YIEGLFLSSGIIRSPDYTMEQVVAVARKLRVDHGFKGYIHLKTIPEANQALIDEAGRW
                     ADRISINIELPANEDLQRLAPEKNGTRIEGAMTAIRERIDASREEREQSTRAPSFAPG
                     GQSTQMIVGATDATDTTILSRASSLYQKQGLRRVY"
BASE COUNT          224 a          399 c          299 g          181 t
ORIGIN      
        1 tcgctacttg ccgtgatcgg cgccggactc gcgatggcgg cgtggcgcga gcggctgacg
       61 gccgagtgga tcttcctcgc tgcggccgcg ccgctcgtgc tcgcaatcgt cgacatcgtg
      121 tttgtcacgc gagacgtgat ccctccgatc tacctcgccg atgctgccgg cgaactcgct
      181 ctcgccgcgc tttggtgcgt gatcgcaatc gcgcgggtgc aaaacaaccg tgccggaaaa
      241 acgcgctgaa cctgctctcg cgaaaccagc tacacacggc tgctccaaca cagagcgcca
      301 caccttgcaa gcgcaccgct cgttcccacg ttgcgggccc gccaatggac ctgcagcaga
      361 aactctcgat cctcgccgac gccgcgaaat acgacgcctc gtgcgcgagc agcggcacca
      421 cgctgcgcaa ctcgcgcgag tccggcggca tcggctccac cactgccacg ggcatctgcc
      481 acagctacac accggacggc cgctgcgttt cgctgctgaa aatcctgctc acgaactact
      541 gcacctacga ctgcctctac tgcgtgaacc gcatctcgag cagcgtgccg cgcgcgcgtt
      601 tcacgcccga tgaagtggtg aacctcacgc tcgatttcta ccggcgaaat tacatcgaag
      661 gcctcttcct cagctcgggc atcattcgca gtcccgacta cacgatggag caagtcgtcg
      721 ccgtcgcccg aaaactccgc gtcgatcacg ggttcaaagg ctacatccac ctgaaaacaa
      781 tccccgaagc gaatcaggcg ctgatcgatg aagcgggtcg ttgggccgat cgcatcagca
      841 tcaacatcga gcttcccgcc aacgaagatc tccaacgcct cgcccccgaa aaaaacggca
      901 cgcgcatcga gggcgcgatg accgcgatcc gtgaacgcat cgacgcgtcc cgcgaagaac
      961 gcgagcaaag cacccgcgcg ccctcgttcg cgcccggcgg ccagagcaca cagatgatcg
     1021 tcggcgcgac cgatgcgacc gacaccacga ttctttctcg cgcctcgagc ctctatcaaa
     1081 aacaaggcct ccgccgtgtt tac
//
LOCUS       DASPLT010000480         1139 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_190838, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000480 DASPLT010000000
VERSION     DASPLT010000480.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1139)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1139)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1139
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_190838"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1139)
                     /locus_tag="VEA63_04945"
     CDS             complement(<1..>1139)
                     /locus_tag="VEA63_04945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GNVR domain-containing protein"
                     /protein_id="HYD83370.1"
                     /translation="NEIRSAEDLNTQVKILESATIVQKVAERLTGDDLRQFLAPYQKE
                     GEPEPPSAAGILALNRKVVPQRLTLILLVQYEHPDRMIAAKVANLFVDEFIAYNARVR
                     IDESMKAVEDLKVRVEQQRRRVDELATDLQTYRSKNNLVSLDERKDIVTEKLKALNML
                     VTQTTSRLKNAEIHLKQVQERRAAGGDLTELPFIASQPLIAQLVQQLSAQKIAMAQLR
                     ERYREKHPRMIEAINSASQTERELTKALKSAEASIESEYQTALRNDQETRRQLAAQEA
                     ESLEIDRYAVEYDNMSRDLQINEQILQSIIGRTRETSMTSTIETQNARIVDLAQPSLK
                     PISPKVGLNLALGVVGGLGLGLAFAFFVAFIDDRVKSSFDIEGVI"
BASE COUNT          205 a          333 c          346 g          255 t
ORIGIN      
        1 caatgacccc ttcgatgtcg aaggagctct tcacgcggtc gtcgatgaag gcgacgaaga
       61 aagcgaaggc gagaccgagg ccgagaccgc cgacgacgcc gagcgcgagg ttcaagccga
      121 ctttcggcga aatgggtttc aagctcggct gcgccagatc gacgatgcgc gcattctgcg
      181 tctcgatcgt gctcgtcatc gacgtttccc gcgtgcgccc gatgatgctt tgtaaaatct
      241 gctcgttgat ctgcaggtcg cgcgacatgt tgtcgtattc gacggcgtag cgatcgatct
      301 cgagcgattc ggcttcttgg gcggccagtt gccgccgcgt ttcctgatcg ttgcgcaaag
      361 ccgtttggta ttccgactcg atcgacgctt cggcggactt cagggctttg gtaagttcgc
      421 gctcggtctg actggcggaa ttgatcgcct cgatcatgcg cgggtgcttc tcgcgatagc
      481 gctcgcgcaa ctgggccatc gcaatcttct gggcggagag ctgctggacg agctgggcga
      541 tcagcggctg gctcgcgatg aagggcaact cggtcaaatc gccgccggcg gcgcgacgct
      601 cctgcacctg tttcaagtga atctccgcgt ttttcagccg ggaggtcgtc tgcgtgacga
      661 gcatgttcaa cgccttcagc ttctcggtga cgatgtcctt gcgctcgtcg agcgacacga
      721 ggttgttctt ggagcgataa gtctgaagat ccgtcgccaa ttcatccacg cgacggcgtt
      781 gttgttcgac gcgaaccttc aggtcctcga cagccttcat cgactcgtcg attcgcacgc
      841 gggcgttata cgcaatgaac tcgtcgacga agagattcgc caccttcgcg gcgatcatgc
      901 ggtccggatg ctcatactgg acgagcaaaa tcaacgtgag tcgctgcgga acgaccttgc
      961 ggttcaacgc gagaataccg gcggcggaag gcggctccgg ctcgccctcc ttttgatacg
     1021 gcgctaaaaa ttgccggagg tcatcgcccg tcagccgttc ggcgactttc tggacgatcg
     1081 tggcgctctc caaaatcttg acctgcgtat tcagatcctc cgcggaacgg atctcgtta
//
LOCUS       DASPLT010000481         1060 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_170481, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000481 DASPLT010000000
VERSION     DASPLT010000481.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1060)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1060)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1060
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_170481"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..886
                     /locus_tag="VEA63_04950"
     CDS             <1..886
                     /locus_tag="VEA63_04950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HYD83371.1"
                     /translation="FVLPIKRAGGEIVWFMPMLPFTSRGSANLRNHRKIAVFDCCTAI
                     VGGHNLARDYMGPTPFRKRFADFGAVIEGPAAALLNEVFIADWCFASRQSADALHHEI
                     PVDAVAEPRGTSDLQVVASGPDVPGDPLYEGILAMIHEAERSLWIVTPYFLPDEVLLR
                     SLKVKIHAGCAVTLILPARSNHPITDLARRHYVRELQSAGARVLLYQPGMLHSKAMII
                     DDRVALFGSANFDPRSLFVNFEVGLFVHSKADVQAIQQWAESLLASCRPPKAERTKKR
                     RIIGTIMEDVSRLLAPLL"
BASE COUNT          198 a          357 c          290 g          215 t
ORIGIN      
        1 gttcgtgctc cccatcaaac gcgccggcgg cgagatcgtc tggttcatgc cgatgctgcc
       61 gttcacctcg cgcggctccg ccaaccttcg caatcatcgc aagatcgccg tcttcgattg
      121 ctgcaccgcg attgtcggcg gccacaacct cgcccgcgat tacatggggc ccacaccgtt
      181 tcgaaaacgc ttcgccgact tcggcgccgt catcgaagga cccgccgccg cgttgctgaa
      241 cgaggtgttc attgccgact ggtgtttcgc cagccgccaa tccgccgacg cgctgcacca
      301 cgaaatcccc gtcgacgcag tcgccgaacc gcgcggcacg agcgacctgc aggtcgtcgc
      361 gagcggcccc gacgtccccg gcgatccgct ttacgagggt attctcgcga tgattcacga
      421 agcggagcgc agtctctgga tcgtcactcc gtattttctg cccgacgaag tgctgttgcg
      481 ttcgctcaag gtgaagatcc acgccggctg cgcggtgacg ctcatcctgc ccgcgcgatc
      541 gaatcacccg atcaccgacc tcgcgcgccg acactacgtc cgcgaactac aaagcgccgg
      601 cgcgcgcgtg ttgctttacc agcccggcat gttgcacagc aaagccatga tcatcgacga
      661 tcgcgttgcg ctcttcggct cggcgaactt cgatccgcgc agccttttcg tgaactttga
      721 agtcggcctc ttcgttcact cgaaagcgga cgtgcaggcg attcagcaat gggcggagtc
      781 gctgctcgcc tcctgccgcc cgcccaaagc ggaacgaaca aaaaagcgcc gcatcatcgg
      841 cacgatcatg gaagacgtca gccgtctcct cgcgccgctg ttgtgagtcg ggatcatgca
      901 gttgcccgcg aatcacgcca attgtcgcga ataccgatga agagaaatga tctttctcca
      961 gacagtaacc tggctctgaa gcggatatat ttgattccac ctctctgaaa ttcgcgttca
     1021 ttcgcgtcat tcgcgggagg tcttctgcat gaatacggct
//
LOCUS       DASPLT010000482         2994 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_170493, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000482 DASPLT010000000
VERSION     DASPLT010000482.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 2994)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2994)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2994
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_170493"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..277)
                     /locus_tag="VEA63_04955"
     CDS             complement(<1..277)
                     /locus_tag="VEA63_04955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD83372.1"
                     /translation="MLCFASTSPAAALENDQVIVPKAAAEKSDVSTRPASGSGTLTLV
                     AVLVLACAGGWMLWRGRNGSIANFRPGARAPRNLAVEETRSLGNRQYL"
     gene            complement(409..660)
                     /gene="fliN"
                     /locus_tag="VEA63_04960"
     CDS             complement(409..660)
                     /gene="fliN"
                     /locus_tag="VEA63_04960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HYD83373.1"
                     /translation="MEEKTLDLVLDVKVRVTVQLGSIHLPMREVLELAPGSVVQLTQH
                     ASDPVGLFVNDQLVAYGEVVVVEDNFGIKITELVGSAKS"
     gene            complement(641..1699)
                     /locus_tag="VEA63_04965"
     CDS             complement(641..1699)
                     /locus_tag="VEA63_04965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HYD83374.1"
                     /translation="MADDPNMPSGSTLDQSEIDKLLAQEVAVAPPKQFLIRPDGSRAG
                     VSPQLKVEAYDFRNPVFLSEIELRRLRLLHEDFIRYLSARLSLYLRMELGLKMAKLTT
                     ATYAKFTESLPNPTHLCLFKVDPLVGVGILDVNPRLALTIADRLLGGRGHSVKAERYL
                     TEIEIALLEDVINIVLEEWCAQWKSEQELHPIIIGHENNGRFLQTSPKDAIILILTLE
                     ANFGDCSEQIQFGVPYYTIEPVVKKMQARRQKDTAVSPAEKKAEWHPAYAGITIPVRA
                     EWDALEVDLREITALRVGDVFELPASIFHETRVSLNGTPKFVGTVGLDSDKVAVQLTR
                     KLPTGNPAPPKSDGRKNS"
     gene            complement(1740..2342)
                     /locus_tag="VEA63_04970"
     CDS             complement(1740..2342)
                     /locus_tag="VEA63_04970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated FliL family
                     protein"
                     /protein_id="HYD83375.1"
                     /translation="MAKAAPSTAPAPAPAAASPTPPAGAAERPAAAASGGGMKAWLPA
                     IAVVVLAPVCTWAVCQFVLIPQLQAQLSATPAAAEASSEGGHGGSHGGGHGEEAKDAA
                     PNYEFQNVVVNLAGTMGTRYLKTSFLVTGAEPNIKSLFEANKPRLTDVTLNVLSSLSL
                     SDLEEPGAKNVLREKLVAAYNQALGKKVAEQVYFSDFVVQ"
     gene            complement(2435..>2994)
                     /locus_tag="VEA63_04975"
     CDS             complement(2435..>2994)
                     /locus_tag="VEA63_04975"
                     /inference="COORDINATES: protein motif:HMM:TIGR03506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein"
                     /protein_id="HYD83376.1"
                     /translation="AQAESDALSDAYTAAQAAQGTGTAAEQLTNVQTSLGSSAAVATT
                     EVVAATTAATAAATPSDRASFSIDKLGNIVETYGNGSSVVTGRVLLQNFNDPSALMKE
                     GKNMYSGFAAAGPVGGTTSLSSANNAPGASGLGTIQAGTLELSNVDLTEQFSELITTQ
                     RSFQAGSRLITVSDSVLEDIVNLKR"
BASE COUNT          524 a          818 c         1094 g          558 t
ORIGIN      
        1 cgagatactg gcggttgccg agcgagcggg tttcctcgac ggcgagattg cgcggggcgc
       61 gggctcccgg acgaaagttg gcgatgctgc cattgcggcc gcgccacagc atccagccgc
      121 cggcgcaggc gagaacgagc acggcgacga gcgtcagcgt gcccgagccc gaagcggggc
      181 gcgtggacac atcggatttt tcggcggcgg ctttcggcac gatgacctga tcgttttcga
      241 gcgccgcagc cgggctggtg gaagcgaaac agagcaaggc gaagcacgcg gcgaaaacgt
      301 ggcgcaggat gcgagggcga acggagcggc gatcccgctc ctcacggagc gagccacgca
      361 gggccgggtg tagctctgcg cgtaagcgca ggaatgcgag gcaacgcttc acgatttcgc
      421 gctaccgacg agctcggtga ttttgatgcc gaagttgtct tcgacgacga cgacctcgcc
      481 gtaggcaacg agctggtcgt tgacaaagag tcccacgggg tcgctcgcgt gctgcgtgag
      541 ctgcacgacg gagccgggag caagctcgag cacctcgcgc atcggcaggt gaatcgagcc
      601 gagttgcacg gtgacgcgga cttttacgtc caggacgaga tcaagagttt tttcttccat
      661 cggatttggg aggggccggg ttgcccgtcg ggagcttgcg ggtgagttgc acggcgacct
      721 tgtcggaatc gagtccgacg gtgccgacga atttcggcgt gccgttgagc gacacgcgcg
      781 tttcgtgaaa aatcgaagcg ggcaactcga agacgtcgcc gacgcgcagc gcggtgatct
      841 cgcggagatc gacctcgagc gcatcccact ccgcgcgcac cggaatggtg atgccggcgt
      901 aggcgggatg ccactcggcc ttcttctcgg cgggagaaac agcggtgtct ttctggcggc
      961 gcgcctgcat tttcttcacg accggctcga tcgtgtaata gggcacgcca aattggatct
     1021 gctcggagca gtcgccgaaa ttcgcctcga gggtgagaat gaggatgatt gcgtccttcg
     1081 gcgaagtttg caggaagcgg ccgttgttct cgtgaccgat gatgatcggg tggagctcct
     1141 gttcgctctt ccactgcgcg caccactctt cgaggacgat gttgatcacg tcctcgagca
     1201 gcgcgatttc aatttcggta agatagcgtt ccgctttgac cgaatgtcca cggccgccca
     1261 ggaggcggtc ggcgatcgtg agcgcgaggc gcgggttgac gtcgaggatg ccgacgccga
     1321 cgagtggatc gactttgaag aggcatagat gggtgggatt gggcagcgac tcggtgaact
     1381 tcgcgtaagt ggcggtggtg agcttcgcca tcttcaggcc gagctccatg cgcagataaa
     1441 gcgagagccg tgcggagaga taacggatga aatcctcgtg cagcagacgg agccggcgaa
     1501 gctcgatttc ggaaaggaaa accgggttgc gaaaatcgta ggcctcgacc ttgagctgcg
     1561 gcgacacgcc ggcgcgcgag ccgtccgggc gaatcaggaa ctgcttcggc ggcgcaacgg
     1621 ccacttcctg cgcgagcagc ttgtcgatct cggattgatc gagcgtgctg ccgctgggca
     1681 tgttgggatc gtcggccatc ggagtcggag atcggagttc gggcggcggg agcgtccgct
     1741 tactgcacga cgaagtcgga gaagtagact tgctccgcga ctttcttgcc gagggcctgg
     1801 ttgtaggcgg cgacgagttt ttcgcgcagg acgttcttcg ctccgggttc ctcgaggtcg
     1861 gagagcgaga gcgacgagag gacgttgagc gtgacgtcgg tgagccgcgg cttgttggcc
     1921 tcgaagagcg acttgatatt gggctcggcg ccggtgacga gaaaactggt tttcaaatag
     1981 cgcgtgccca tcgtgccggc gaggttaacg acgacgttct gaaactcgta attgggcgcg
     2041 gcgtccttgg cttcctcacc gtggccgccg ccgtggcttc cgccgtgtcc accttcggaa
     2101 gaagcttccg cagcggccgg cgtggcggag agttgcgcct gtagttgagg aatcaatacg
     2161 aactggcaga cggcccacgt gcagacgggt gcgagcacca cgacggcgat ggccggcagc
     2221 caggctttca taccgccgcc gctggcggcg gctgcgggcc gctcggcggc gccggcggga
     2281 ggcgtcgggc ttgcggctgc gggagcaggc gcgggagcgg tggaaggtgc ggctttggcc
     2341 atgaggtgag ttgaggatta agggtggttc gttgagagag cggagtgagg aggttgggaa
     2401 acgctcggcg ctcagttctc aacgttcatc cggcttagcg cttcaggttg acgatgtctt
     2461 cgaggaccga gtcggagaca gtgatcaaac gcgatccggc ctggaagctg cgctgggtgg
     2521 tgatcagttc ggaaaactgt tcggtgaggt cgacgttcga gagctcgagc gtgcccgcct
     2581 gaatcgtgcc gagcccgctc gcgccgggag cgttgttggc actgctgagc gaggtggtgc
     2641 ctccgaccgg cccggcggcg gcgaagccgg aatacatgtt tttgccttcc ttcatcagcg
     2701 cggatgggtc attgaaattt tgcagcagca cacggccggt gacgacggac gagccgttgc
     2761 cgtaggtttc gacaatgttg ccgagcttat cgatggagaa gctcgcgcgg tccgacggtg
     2821 tggcggcggc cgtcgcggcc gtggtcgcgg cgacgacttc ggtggtcgca acagcggcgg
     2881 agctgccgag cgacgtctgg acgttggtaa gctgttcggc ggcggtgccg gtgccttgcg
     2941 cggcttgcgc agcggtgtag gcatcggaga gcgcgtcgct ctcagcttgg gcga
//
LOCUS       DASPLT010000483         1567 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_210847, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000483 DASPLT010000000
VERSION     DASPLT010000483.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1567)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1567)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1567
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_210847"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            230..1105
                     /locus_tag="VEA63_04980"
     CDS             230..1105
                     /locus_tag="VEA63_04980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007358812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar nucleotide-binding protein"
                     /protein_id="HYD83377.1"
                     /translation="MIYLLGGSGYVGHAYQSLLTRKGIPFRNLRRAEIDYTYPGTLAA
                     ALRADRPEFLINAAGYTGKPNVDACELHKTECLFGNAVLPGLVAQACADAGVPWGHVS
                     SGCIYTGARPDGRSFTETDAPNFSFRQNNCSFYSGTKALGEEVLAGKPDVYIWRLRIP
                     FNEVDNPRNYLTKLLRYQNLLEATNSISQLEEFVAATFACWEKRIPFGTYNVTNPGQI
                     TTHEVVELIKKTGVSDKSFTFFKDEADFMQKAAKTPRSNCVMDSSKLAAAGITLTEVH
                     AAVERDLRRWQPESK"
     gene            1122..1487
                     /locus_tag="VEA63_04985"
     CDS             1122..1487
                     /locus_tag="VEA63_04985"
                     /inference="COORDINATES: protein motif:HMM:TIGR02436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HYD83378.1"
                     /translation="MNTSDQLQRFGGYQKALELFDFVVADSDLLRQHPTLHRLISQQY
                     PSADSIAANIEEGFGRGSQREYAQFLVIARGSTQETAGRYQRFKHWLPPDVIESRVNL
                     CGEIIGILSASIATLRRSR"
BASE COUNT          328 a          598 c          353 g          288 t
ORIGIN      
        1 tgcgcgtgag cgcagggatc gtccgctcgc gcgttctctg atcggcgaac gcgagcgaaa
       61 ccacgcctat cggaaattcg cgccaaaatc cggtcccttt cgcctttttc gtggttccac
      121 aaaccaccca ccccgcccaa ccacttcgcc cgctccagtt tccactgacc gcacgctcag
      181 ggaaaaacct tgttcactcc gcgcccgttt cacttcactg ctccttccca tgatttatct
      241 gctcggaggt tccggttacg tcggtcacgc ctatcagtcg ctgctcacgc gcaaaggcat
      301 cccctttcgc aatctccgcc gcgccgagat cgactacacc tatcccggca ccctcgccgc
      361 cgccctccgc gccgaccgcc ccgagttcct gatcaacgcc gccggctaca ccggcaaacc
      421 caacgtcgac gcctgcgagc tccacaaaac cgaatgcctc ttcggcaacg ccgtcctccc
      481 cggcctcgtc gcccaagcct gtgccgacgc cggcgtcccc tggggccacg tttcctccgg
      541 ctgcatctac accggcgccc gccccgacgg ccgcagtttc accgagaccg acgcccccaa
      601 tttctccttc cgccaaaaca actgctcctt ctacagcggc acgaaagcgc tcggcgaaga
      661 agtcctcgcc ggcaaacccg acgtctacat ctggcggctc cggatcccgt tcaacgaggt
      721 cgacaacccg cgcaactacc tcaccaagct gctgcgctac caaaacctcc tcgaagccac
      781 gaactccatt tcccaactcg aagaattcgt cgccgccacc ttcgcctgct gggaaaaacg
      841 catccctttc ggcacctaca acgtcacaaa tcccggccag atcacgacgc acgaagtcgt
      901 tgagctcatc aagaaaaccg gcgtctctga caaatcgttc acgttcttca aagacgaagc
      961 cgacttcatg caaaaggccg cgaagacccc gcgttcgaac tgcgtgatgg actccagcaa
     1021 actcgccgcc gccggcatca cgctcaccga agtccacgcc gccgtcgaac gcgacctccg
     1081 ccgctggcag ccagagtcaa agtgagagtg agagtgaaag agtgaacacg tccgaccagc
     1141 tccagcgatt cggtggctac caaaaggctc tcgaactttt cgacttcgta gtcgcggact
     1201 ccgatctcct gcggcaacac cccacccttc accgtttgat atcacagcaa taccccagtg
     1261 cggattcgat cgcggcaaac atcgaagaag gattcggccg cggaagccaa agagaatacg
     1321 cccagtttct tgtgatcgcc cgcggatcta cacaagaaac cgccgggcgc taccagcgct
     1381 tcaaacactg gctgcctccc gacgtgatcg aatcccgcgt aaacctctgc ggcgaaatca
     1441 tcggcatcct ttcagcctcg atcgccaccc tccgccgttc ccgctaactc ggtctcactt
     1501 tcactctcac tttccctttc acttccagca tgaacctcct cgtcaccggc ggctgcggct
     1561 tcatcgg
//
LOCUS       DASPLT010000484         2374 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_230296, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000484 DASPLT010000000
VERSION     DASPLT010000484.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 2374)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2374)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2374
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_230296"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(227..559)
                     /locus_tag="VEA63_04990"
     CDS             complement(227..559)
                     /locus_tag="VEA63_04990"
                     /inference="COORDINATES: protein motif:HMM:NF017451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HYD83379.1"
                     /translation="MANNDRLHQFGAYRKACELFDHVVADMTPLARNPLCMRLVSHQF
                     ASADSIGSNIEEGYGRSTRKDYTHFLVIARGSATETRGRYEQRFKHWLPAPTIAARVA
                     LCEESSPS"
     gene            complement(564..1283)
                     /locus_tag="VEA63_04995"
     CDS             complement(564..1283)
                     /locus_tag="VEA63_04995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HYD83380.1"
                     /translation="MSAAFPVALLAGGLATRLRPITEKIPKLLVDVAGEPFFTHQLRL
                     LKKNGLTHVVVCAGYLGEQIVERYGDGSAHGMRIEYSFDGEKLLGTGGALIRALPKLG
                     ETFYVLYGDSYLPIEYQTVGNFFLGSGKLGLMTVFENRERYDASNVWFEGGEIKRYDK
                     KARLPQMHHIDYGLGVFRAKAFAGYPHGAPVDLAEVQKALVLRGELAGYEVTHRFYEI
                     GSPAGLAELDVLLQGNEGVRE"
     gene            complement(1285..2268)
                     /locus_tag="VEA63_05000"
     CDS             complement(1285..2268)
                     /locus_tag="VEA63_05000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HYD83381.1"
                     /translation="MIITRSPLRVSLGGGGTDLPSYYSKHGGFLVAAAIDKYVYITKH
                     RTFQQEIIIKYSKLERVQSVDQIEHPIVREALKLTGVTEPHLELASMADIPGGTGLGS
                     SGSFTTALLKALHAFKKNLISPAELAEQACHIEIDRLGEPIGKQDQYIAAVGGITAFT
                     FHRDGRVEYRPCKISEETLFNLEDNLLLFFTGYSRSASAILKDQNDKSKQQDAAMLEN
                     LHFTKELGLKSLDCLESGNLDEFARLMDVHWQRKKIRSTGMSNTHINEWYDHAMANGA
                     LGGKLIGAGGGGFLMFYAGDKAKLRHAMRSQGLQEVRFRFDFEGTKVVSQN"
BASE COUNT          439 a          613 c          836 g          486 t
ORIGIN      
        1 tggccggcgt tggcggcgct gccgccgacg ccgaggatga aaagccgtcc gccggaggcg
       61 cgcgtggcga cgagcgtctg cacgcatttc tcgcagtcgg cgggattcag ttgggcgacg
      121 atctcggcgg tttcgcgcag gtgttggaga gagtaggaca tgagaggaaa gggaatgagg
      181 gagtgagggc cctatgggcg ggagcggagg gtgacgatgg aggcgctcag gatggcgatg
      241 attcttcgca gagcgcgact cgggcggcga tggttggcgc gggaagccag tgtttgaaac
      301 gttgttcgta gcggccgcgg gtttcggtcg ctgagccgcg cgcgatcacg agaaaatgcg
      361 tgtagtcttt tcgcgtgctg cgtccgtagc cctcttcgat gttggagcca atcgaatcgg
      421 cgctggcaaa ttggtgagag accaggcgca tgcagagagg attgcgggcg agcggcgtca
      481 tatcggcgac aacgtggtcg aacagctcac aagcttttcg gtaagcgccg aattgatgaa
      541 ggcggtcgtt gttggccatg ctctcactcc ctcactccct cattcccttg taacagcaca
      601 tcgagttcgg ccaggccggc gggggagccg atttcgtaga agcgatgagt gacctcgtag
      661 ccggcgagct cgccgcggag gacgagcgct ttttggactt cggcgagatc gacgggcgca
      721 ccgtgcgggt aaccggcgaa ggctttggcg cgaaagacgc cgagaccgta gtcgatgtgg
      781 tgcatctgcg ggaggcgcgc ttttttgtcg tagcgtttga tctcaccgcc ttcgaaccag
      841 acgttgctcg catcgtagcg ttcgcggttt tcgaagacgg tcatcaggcc gagcttgccg
      901 gagccgagaa agaagttgcc gacggtttgg tattcgatcg gcaaatacga gtcgccgtag
      961 agcacgtaaa acgtctcgcc gagtttcggc agcgcccgga tcaacgcgcc gccggtgccg
     1021 agaagttttt ccccgtcgaa ggaatattcg atgcgcatgc catgcgcgga gccgtcgccg
     1081 taacgctcga cgatttgctc gccgagataa ccggcgcaca cgacgacatg cgtgagaccg
     1141 ttcttcttta atagccggag ttggtgcgtg aagaacggct cgccggcgac gtcgacgaga
     1201 agcttgggaa tcttctcggt gatggggcga aggcgggtgg cgaggcctcc cgcgagcagg
     1261 gcgacaggaa aggcggcgga cattttagtt ctgcgaaacg actttggtgc cctcgaagtc
     1321 gaagcggaag cgcacttcct ggaggccttg ggaacgcatg gcgtggcgga gtttcgcttt
     1381 gtcgccggcg tagaacatga gaaagccgcc gccgccagcg ccaatgagtt tgccgccgag
     1441 agcgccgttg gccatcgcgt ggtcatacca ttcgttgatg tgcgtgttgc tcatgccggt
     1501 ggaacggatt tttttgcgct gccaatggac atccatgagg cgggcaaatt cgtcgaggtt
     1561 gccggactcg agacaatcga gcgacttcag gccgagttct ttcgtgaaat gcagattctc
     1621 gagcatcgcg gcatcctgct gcttcgattt gtcgttttga tccttgagga tcgcggatgc
     1681 gctgcgcgag tagccggtga agaagaggag caggttgtcc tcgaggttga agagcgtctc
     1741 ctccgagatc ttgcaggggc ggtattcgac gcgtccatcg cgatgaaacg tgaacgcagt
     1801 gatgccgccg acagcggcga tgtattgatc ctgtttgccg atcggttcgc cgaggcgatc
     1861 gatctcgatg tggcaggcct gctcggcgag ttcggcgggc gagatcaggt tttttttgaa
     1921 ggcgtgcagc gccttgagca gggcggtcgt gaagctgccg ctcgagccga ggccggtgcc
     1981 gcccgggatg tcggccatcg aggcgagctc gaggtgcggt tcggtgacgc ccgtcagctt
     2041 cagcgcctcg cgcacgatcg ggtgctcgat ctgatcgacc gactgcacgc gctcgagctt
     2101 ggagtatttg atgatgatct cctgctgaaa cgtccggtgc ttcgtgatgt aaacgtattt
     2161 gtcgattgcg gcggcgacga gaaatccacc gtgtttggaa taataggaag gcaggtcggt
     2221 gccgccgccg ccgagggaaa cgcggagcgg ggaacgggtg atgatcatgg cgaaagcgtg
     2281 gcgtcggaga ccgcgtaacc gctctggcgg tagatgcgtt ctacgatatc gagaatttga
     2341 atacctgcgg cgaggcccgg cgacggttcg cggg
//
LOCUS       DASPLT010000485         2259 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_293082, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000485 DASPLT010000000
VERSION     DASPLT010000485.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 2259)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2259)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2259
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_293082"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(185..1447)
                     /locus_tag="VEA63_05005"
     CDS             complement(185..1447)
                     /locus_tag="VEA63_05005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007363860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2851 family protein"
                     /protein_id="HYD83382.1"
                     /translation="MSAEFEMQSVARTVAPLNAVAELQGIYGPFSFHEKLLQKIWLRG
                     DFDRARLVATDGRRVTVLHAGRWNLLGGPDFSNARLRFGDEPEITGDVELHLHASDWA
                     AHGHARDRAYDGVRLHVVLFPPRNDHVTRGADSRAIPVVALLPLLHHDLEEYAADEAV
                     EGLANRPASQIMETLGVLPGAELATLLERHARARWTQKVHFARLRVQRLDWSEACHQT
                     AMEILGYRFNRAPMLRVATAHPLDEWASPIFSPDAVFESERAGWSLHGLRPANHPRQR
                     LRQYATWVHARPDWPERLRTFAKRLPRVETTTLTGDVRRAERFTILRRDWAQEICAEA
                     VGGTRFDNLVCDGFLPLLATLNAAEGEGTWHHWFAGDLPPVLPRALRDLGVFGGRQNP
                     ACHGAAPGLLGWLLERERCGTSPQGRGA"
     gene            complement(1444..2004)
                     /locus_tag="VEA63_05010"
     CDS             complement(1444..2004)
                     /locus_tag="VEA63_05010"
                     /inference="COORDINATES: protein motif:HMM:NF017774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HYD83383.1"
                     /translation="MSASTITGREFHVIAAKEVESLRAGRQDCSVSFVRHLLVDGSNI
                     LHAWPELRSLAKRDRDTARAQLVQRLAAIHDADRVRVTIVFDGRGAELSIDRPSAEPT
                     FSVVHTSSSLTADDVIEQMVSGSTDATACIVATDDLAERSMVAAARGHTLTSDDLATW
                     ARQAEARQAAALEKRRAANQQEWRKP"
     gene            2066..>2259
                     /locus_tag="VEA63_05015"
     CDS             2066..>2259
                     /locus_tag="VEA63_05015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD83384.1"
                     /translation="MTPHIGKLVSFGIVILILVIAASQATFVVQPGFRGVEVTLGKVS
                     DQFKPEGFGTKAPFITHIISV"
BASE COUNT          435 a          779 c          644 g          401 t
ORIGIN      
        1 gatcatgtcc gcgcgcggga tccccttctc cttctccatg tattctagaa ctgaaaggat
       61 ttcgctactc ataggatgaa agtgatgggc aaaagttggg aaaaaaaaag cgggccgcag
      121 ggcccacttc ttggaaagag agtggtatag agtcggtgaa cttattcggc ggcccgaaac
      181 cttgtcaagc cccgcgcccc tgcggagacg tgccgcaccg ctcgcgctca agtaaccagc
      241 cgagcaaacc tggcgcagca ccgtgacacg ccggattctg ccgaccgccg aacacgccca
      301 agtcacgcaa tgcgcgcggc aaaaccggcg gcagatcgcc cgcaaaccaa tggtgccaag
      361 tcccctcgcc ttcagcggcg ttcagcgtgg ccagcagcgg cagaaaccca tcgcacacca
      421 gattgtcgaa gcgcgtgccg cccaccgcct ccgcgcaaat ctcctgcgcc caatcccgcc
      481 gcaggatcgt gaaccgctcc gctcgtcgca cgtcgccggt aagcgtcgtc gtctccacgc
      541 gtggcagccg cttcgcaaac gtccgcaacc gctccggcca gtccggccgc gcgtgcaccc
      601 aggtcgcgta ttgccgcagc cgttgccgcg gatgattcgc cggacgtagc ccgtgcaaac
      661 tccaccccgc ccgctcgctc tcaaacaccg catcgggcga aaaaatcggc gacgcccatt
      721 cgtccagcgg atgcgccgtg gccacccgca acatcggcgc gcggttgaac cgatagccaa
      781 gaatctccat cgcggtttgg tggcaggctt cgctccagtc gagccgttgc acgcgcaatc
      841 gcgcgaagtg aaccttctgc gtccaacgcg cccgggcatg tcgttcgagc aacgtcgcga
      901 gctccgcccc cggcagcacg ccgagcgtct ccatgatctg cgacgccgga cgattcgcga
      961 gcccctcgac cgcttcgtcc gcggcgtatt cttcgaggtc gtggtgtagc agcggcagca
     1021 gcgcaaccac gggaattgca cgcgagtccg cgccacgcgt gacgtgatcg tttctcggtg
     1081 gaaaaagcac cacgtgcaat cgcacgccat cataagcgcg atcgcgcgca tgaccgtgcg
     1141 ccgcccaatc gctcgcatgc aaatgcagtt ccacgtcccc tgtaatttcc ggctcgtcgc
     1201 caaaccgcag ccgcgcgttc gaaaaatccg ggccgcccaa aagattccag cgcccggcgt
     1261 gcagcaccgt cacacgtcgc ccgtcggtcg ccacgagacg cgctcggtcg aaatcgccgc
     1321 gcagccagat tttctgcagc agcttttcgt gaaacgaaaa tggcccgtag atgccctgca
     1381 gttcagcgac cgcgttgagt ggtgcgaccg ttcgcgcgac ggactgcatc tcgaattccg
     1441 cgctcatggt tttcgccact cctgctgatt tgccgcgcga cgtttctcca acgccgcggc
     1501 ctgccgcgct tctgcctgac gcgcccacgt tgcgagatcg tccgacgtca atgtatgacc
     1561 gcgcgccgcg gcgaccatgc tgcgttcggc gagatcgtcc gtcgccacga tgcaagcggt
     1621 ggcgtccgta gaacccgaca ccatctgctc gatcacgtca tcggcggtca gcgacgacga
     1681 cgtgtgcacc accgagaaag tcggctctgc cgacggacga tcgatcgaga gctccgcgcc
     1741 gcgaccgtcg aagacgatcg tgacgcgcac gcgatccgca tcgtgaatcg ccgcgagccg
     1801 ttgcacgagt tgcgcgcgag ccgtgtcgcg gtcgcgtttc gcgagcgagc gaagttccgg
     1861 ccatgcgtgc aggatgttcg atccatcgac gagcaaatgg cgcacgaagc tcacgctgca
     1921 atcctgccga cctgcgcgaa gcgactccac ttcttttgca gcgatgacat gaaattcgcg
     1981 accggtgatg gtggaagcgc tcatccgttt tccagagaat caatcgcctc ttgcagtgtg
     2041 cgctcgcgta agttcgcatg cgaacatgac tccccacatc ggaaaactcg tcagcttcgg
     2101 tatcgtcatt ctcatcctcg tcatcgccgc gtcgcaggcg actttcgtgg ttcaaccggg
     2161 gtttcgcggt gtcgaggtta cgctcggcaa agtcagcgac caattcaaac ccgagggctt
     2221 cggcacgaag gcgcccttta tcacccacat catttccgt
//
LOCUS       DASPLT010000486         1073 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_251738, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000486 DASPLT010000000
VERSION     DASPLT010000486.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1073)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1073)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1073
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_251738"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..673
                     /locus_tag="VEA63_05020"
     CDS             <1..673
                     /locus_tag="VEA63_05020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-isopropylmalate synthase regulatory
                     domain-containing protein"
                     /protein_id="HYD83385.1"
                     /translation="AHKGGLHANAAQKVARSYEHIDPALVGNRTRVLVSDMAGRSSLA
                     LKAREMGLELDEKKPEMKGLVEELKEREFRGYEYEAADASFKLLVAKWLGQRKSYFEV
                     ESYRVIIERHGEELWAEATVKLKVNGETVHTVAEESGPIGALDKALRMALDKAYPQLR
                     EMTLRDYKVRILESNAGAGSRTRVLIESGDGARIWGTVGVSDNIIDASWEALRESVEF
                     KLTQA"
     gene            763..>1073
                     /locus_tag="VEA63_05025"
     CDS             763..>1073
                     /locus_tag="VEA63_05025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA desaturase"
                     /protein_id="HYD83386.1"
                     /translation="MPFRIPTARINWTNSSFLIGTAIITLTAVPVYLWFYGVDAFQLS
                     LFFAFFIATGLSITLGYHRLFAHVAFQASWPVRLVTLIFGAAAFENTALMWVSDHRRH
                     H"
BASE COUNT          205 a          279 c          374 g          215 t
ORIGIN      
        1 cgcgcacaaa ggcggattgc atgcgaacgc ggcgcagaag gtggcgcgca gctacgagca
       61 catcgatccg gcgttggtgg gcaatcgcac gcgcgtgctt gtatccgaca tggcaggacg
      121 cagcagcctg gcgttgaagg cgcgcgagat ggggctggag ctggacgaga agaagccgga
      181 gatgaaaggc ctcgtggagg agttgaagga gcgggagttt cgcggctacg aatacgaggc
      241 ggcggatgcg tcgttcaagc tgctggtcgc gaaatggctc gggcagcgga agagttattt
      301 tgaagtggag agctaccgcg tgatcatcga gcggcacggc gaggaattgt gggcggaagc
      361 gaccgtgaag ttgaaggtga atggcgagac ggtgcatacg gtggccgagg agagcgggcc
      421 gatcggcgcg ctcgacaagg cgctgcgcat ggcgctggac aaagcttatc cgcagctgcg
      481 cgagatgacg ttgcgcgact ataaagtgcg cattctcgag agcaatgcgg gcgccggttc
      541 gcgcacgcgc gtgctgatcg agagcggtga tggggcacgc atttggggga ccgtgggcgt
      601 cagcgacaac atcatcgacg cgagctggga agcgttgcgc gaatcggtgg aattcaagct
      661 gactcaggcg tagttcggag gcgtgaatgc atggaatttt ccatgggcat tccttgaatt
      721 tctgctggcg gatcggcgcg agcgcttcac gctgtgatcg acatgccatt tcgtattccg
      781 accgcgcgca tcaattggac gaatagttcc ttcctgatcg gcaccgctat catcacgctc
      841 accgccgttc ctgtttatct ctggttctac ggcgtggacg cctttcagct ttcgctgttc
      901 ttcgcgtttt tcatcgcgac cggcctgagc atcacgctgg gttatcaccg gcttttcgcg
      961 cacgtcgcgt ttcaagcgag ctggccggtg cggctggtga cgttgatctt cggcgcggcg
     1021 gccttcgaaa acaccgccct gatgtgggtc tcggatcatc gccggcacca caa
//
LOCUS       DASPLT010000487         1527 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_271850, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000487 DASPLT010000000
VERSION     DASPLT010000487.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1527)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1527)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1527
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_271850"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            437..>1527
                     /locus_tag="VEA63_05030"
     CDS             437..>1527
                     /locus_tag="VEA63_05030"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HYD83387.1"
                     /translation="MNHRFPSAHARRPVAFAALAAAFALATAHPTHAQDLSPSSPLLA
                     SADAGGAGAPIHLARYEVSERGVTRANNIISLPDIAPTTPAGTNPLLFLNRLPGVNNN
                     ASSNFGIRGTDGTGLRLRAFTLNVIGTAVDGIPVSTSNGFQSQPPTRLIDSENLSSVV
                     VSQGSGDVTTPSYSALGGSINFFTRAPAADPGAEFTATYGSREFQRVFFRTDSGNLGH
                     GLSAFVSGSTARQITSFTDSDLPEHKRRKLDAQLQYETPLLLLNFGISHSKSDDHDDR
                     PVSGSYFGNWIPYAPGGPTGDLSDRGRRWFYPTFDDGNPNGLSSVNYDKNRNGYTETL
                     YRLRAVLTPSADVSVTAIPYYQDRDAYHFG"
BASE COUNT          277 a          612 c          356 g          282 t
ORIGIN      
        1 cgccgccttc gccacgccca tcgggtatca aaccaacacc tacgtctacg gcatcggcgg
       61 ctacaaattc cgcgacttca tgcgcatcgg cattccgctg aacatcctct gtttcatcgt
      121 cgcgatgctc attgtcccaa aagtctggcc tctctagtgg ctgccgaagc accgtcatac
      181 gtccctataa gtcctataag acctataggt cccatccttc tcgcgctgct ccgccctcag
      241 atttaattcc tactattccg ctttacattc gcgggatctt atattctttc cgccatggct
      301 ccacgctgca cagcgcgagc cggatgaccc gagtcagttc tggccgacca cggcagacga
      361 ccgggtgaca cacatcaccg acgaatcccc cggacgaccg ggaccgcatt cgccctcccg
      421 ttcaggaaac caaactgtga atcatcgttt tccgtccgcg catgcgcgtc ggcccgtggc
      481 gttcgccgcg ctcgccgccg cgtttgccct cgccaccgca caccccacac acgcccaaga
      541 cctgtccccg tcttcgcctc tgctcgcctc cgccgatgcc ggcggggctg gcgcgccaat
      601 ccacctcgcg cgctacgaag tgtccgagcg cggcgtcacg cgcgcgaaca acatcatcag
      661 ccttcccgat atcgcaccga ccacgccggc gggcacgaat ccgctgctct tcctcaatcg
      721 cctgcccggc gtgaacaaca acgcctcgag taatttcggc atccgcggca ccgatggcac
      781 cggcctccgc ctccgcgcct tcacgctcaa cgtcatcggc accgccgtcg acggcatccc
      841 cgtttccacg agcaacggct tccagagcca gccgcccacg cgcctgatcg acagcgaaaa
      901 cctcagcagc gtcgtcgtct cccaaggctc gggcgatgtc accacgccgt cctactccgc
      961 gctcggcggc tccattaatt tcttcacccg cgcgcccgcc gccgatcctg gcgccgagtt
     1021 caccgccacc tacggctcgc gcgaatttca acgtgtcttc ttccgcaccg acagcggcaa
     1081 tctcggccac ggtctcagcg ctttcgtcag cggctccacc gcgcgccaga tcacgagttt
     1141 caccgacagc gatcttcccg agcacaaacg ccgaaaactc gacgcccagc tccaatacga
     1201 gacgccgctc ctcctgctca acttcggcat cagccactcg aagtccgacg accacgacga
     1261 tcgccccgtt tccggcagct acttcggcaa ctggattccc tacgcccccg gcggaccgac
     1321 cggcgatctc tccgaccgcg gtcgccgctg gttttatccg acgttcgacg acggcaatcc
     1381 caacggcctc tcgtccgtca attacgacaa aaaccgcaac ggctacaccg agaccctcta
     1441 ccgcctccgc gccgtcctca cgccctccgc cgacgtatcc gtcaccgcga taccctacta
     1501 ccaggatcgt gacgcctatc acttcgg
//
LOCUS       DASPLT010000488         1219 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_210971, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000488 DASPLT010000000
VERSION     DASPLT010000488.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1219)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1219)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1219
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_210971"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..202)
                     /locus_tag="VEA63_05035"
     CDS             complement(<1..202)
                     /locus_tag="VEA63_05035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HYD83388.1"
                     /translation="MNLLQLRQSGHWPTLLTSFLYFDVSFMVWTILGALGAMIAPSLG
                     LTAQEKFLMVSTPILAGAFLRIV"
     gene            complement(612..>1219)
                     /locus_tag="VEA63_05040"
     CDS             complement(612..>1219)
                     /locus_tag="VEA63_05040"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYD83389.1"
                     /translation="DGEVRVAVVPPAQMFRNLEAGTIDGYCAGEPWNTIAVRAGAGWC
                     PMWSAAHAPGHVEKVLMVTEGFAKTRGAEHAALIAALHDAAVWCDAPENREELSKLLS
                     AAVFLNLPARVIAPALLGRFDCGHGRVEDVPDFVVFHRGEANAPTIAKATALQNELAA
                     AGILPVDVDPELPRRLFREDVYRDALNPHRTHEPPAVPHLR"
BASE COUNT          241 a          404 c          375 g          199 t
ORIGIN      
        1 acacgatgcg cagaaacgcc cccgcgagga tcggcgtcga caccatcaaa aatttttcct
       61 gcgccgtcag tcccagcgat ggcgcgatca tcgcgcccaa cgcgccgaga atcgtccata
      121 ccatgaaact cacgtcgaaa tagaggaacg acgtcagcag tgtgggccag tgacccgatt
      181 gtctgagttg gagaagattc atatcttggc gcgctccatg agcagcgctc acgccaagga
      241 ggagtcggcg gcgatttgcc cgatgaatag tttttcgccg agcaatgaac gcggttcatc
      301 gacccggcgt cctccggctc gccgacgata ccacgcggtg gcgtcgcaaa tcaggtggct
      361 cgctgcgtga gcagtgggat gtttcctgcc tgccgccacc aacgcgcccg tgcacgttga
      421 accacgcgac ggcgatcggc ccgaccgcgc ctcgttccca tcgccgtcag ggccaacgtc
      481 caagagtggg gttgatcaag gtcgaccaga gcgagcaggc cgccacgtga tcgcgaagcc
      541 gagaagggcc ttttcgagca acgcgtcgac aggattatag agcgcgctgg cgagaatcat
      601 cccgatcagc tctaacggag gtgcggaacg gcaggcggtt catgcgtgcg atgaggattc
      661 aaggcgtcgc gatacacgtc ttcgcgaaac agacggcggg gtaattcagg gtcgacgtcg
      721 accggcagga tgcccgcggc ggcaagttcg ttttgaagtg cggtcgcttt cgcgatcgtc
      781 ggcgcgttcg cttcgccgcg gtgaaagacg acaaagtccg gcacgtcttc cacgcggccg
      841 tggccgcagt cgaagcgacc gagcagcgcc ggcgcgatca cgcgcgcggg aaggttcaag
      901 aaaacggccg cgcttaaaag cttcgagagc tcctcgcgat tctccggcgc gtcacaccag
      961 acggcggcgt catgcaacgc cgcgatcaac gccgcgtgct ccgcgccgcg cgtctttgca
     1021 aaaccttccg tgaccatcag cactttctcc acatgacccg gcgcgtgcgc cgcgctccac
     1081 atcgggcacc aacccgcgcc cgcgcgcacc gcgatcgtgt tccacggttc gcccgcgcaa
     1141 taaccgtcga tcgtgcccgc ctccaggttg cgaaacatct gcgccggcgg cacgaccgcg
     1201 acgcgcacct cgccatcgc
//
LOCUS       DASPLT010000489         1221 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_29290, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000489 DASPLT010000000
VERSION     DASPLT010000489.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1221)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1221)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1221
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_29290"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..205)
                     /locus_tag="VEA63_05045"
     CDS             complement(<1..205)
                     /locus_tag="VEA63_05045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD83390.1"
                     /translation="MLPLFSSLVPSAALALLLSAAIFAAPPPRPWSPDPFTPLTPERI
                     AALPPAERTPWQAYWNASEQLLAK"
     gene            538..>1221
                     /locus_tag="VEA63_05050"
     CDS             538..>1221
                     /locus_tag="VEA63_05050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HYD83391.1"
                     /translation="MSKQPLVTHIFTADPSAHLFEGRIYIYPSHDLDIDVPENDLGDQ
                     YAMTDYHVLSQPHPEGPATDHGEALHVRDVPWASRQMWAPDAAYRNGRYYLYFPAKDK
                     DGIFRIGVAISARPEGPFKAEPEPIAGSFSIDPCAFRDDDRAFYLIFGGIWGGQLQRW
                     ATGSYQADGIERQGDELALCPKIARLTEDMLGFATTPQDAKIVDATGALLKAGDKERR
                     FFEGPWMHKY"
BASE COUNT          254 a          320 c          429 g          218 t
ORIGIN      
        1 gtttggccag gagctgttcg gaggcgttcc agtaggcctg ccacggcgtg cgttcagcgg
       61 gcggaagcgc ggcgatacgc tcgggggtga gcggcgtgaa gggatcgggg ctccacggac
      121 gcggaggcgg tgcggcgaaa atggcggcgg acaacaagag cgcgagcgcg gcggacggaa
      181 cgagagaaga gaataggggt agcatgataa aaggaacgat ggggcgttgc gatcacatga
      241 cgagcgtgcg gcgcggccgg ataccgcgcg gtcgacaatg agacgggaaa aacggacgcg
      301 cgtccccgtg cgtttctagt tacgggaatt gtccgctgcc ggatcgagcc cgccattctc
      361 gtcgagaaac gtcacgatgc cgaagagacg caacgcgagt cgttcgcgaa acggtgagtg
      421 gaggtcagtc taagattcga ccgtcatcga gatttgggtc gggagaagga tgcgcgacgg
      481 tgttctttcg cacagcgctg ctcgatcaac gagcacgcga ttcagggtta ttcccgcatg
      541 agcaaacaac ctctcgtcac gcatattttc acggcagatc cctcggctca ccttttcgaa
      601 ggacggatct acatttatcc gtcacacgac ttggacatcg acgtgccgga gaacgatctc
      661 ggcgatcagt atgcgatgac ggattatcat gtgttgtcgc agccgcatcc cgaagggccg
      721 gcgacggacc atggcgaggc gctgcatgtg cgcgatgtgc cgtgggcttc gcgacagatg
      781 tgggcgcccg acgcggcgta tcgaaacgga cgctattacc tttattttcc ggcgaaggac
      841 aaagacggga tttttcgaat cggcgtcgcg atttccgcgc ggccggaagg cccgttcaag
      901 gcggagccgg agccgatcgc gggcagtttc agcatcgatc cgtgcgcatt tcgcgacgat
      961 gaccgcgcgt tttacctgat cttcggcggg atctggggcg ggcagttgca acgctgggcg
     1021 acgggcagct atcaagccga cggcatcgag cggcagggtg acgagctggc gctctgtccg
     1081 aaaatcgcgc ggctcacgga ggacatgctg ggctttgcaa cgactccgca ggatgcgaaa
     1141 atcgtcgatg cgacgggtgc gctactaaag gcgggcgaca aggagcggcg gtttttcgaa
     1201 gggccgtgga tgcacaagta t
//
LOCUS       DASPLT010000490         1549 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_149206, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000490 DASPLT010000000
VERSION     DASPLT010000490.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1549)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1549)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1549
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_149206"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(230..604)
                     /locus_tag="VEA63_05055"
     CDS             complement(230..604)
                     /locus_tag="VEA63_05055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HYD83392.1"
                     /translation="MLLFRNGGTEAHAHLTAEQKEQLTRQWNDWYDGLASKGKVQHGR
                     PLGLGGRVVSGALGSKVTDGPYAEAKEVVAGYLFLTVADIDEATAIAKECPGLPIGLT
                     VEVRPVVDASPVLEGVQGHAPE"
     gene            871..1302
                     /locus_tag="VEA63_05060"
     CDS             871..1302
                     /locus_tag="VEA63_05060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HYD83393.1"
                     /translation="MNTITPHLVCAGAADAIEFYKKAFGATEVTRVPGPAGKLIHASI
                     HIGDSTVMLCDEFPDWGNVSPKSLKGTPVTIHITTDNADALAARAAEAGATIVMPVED
                     AFWGDRYGVLEDPFGHRWSVATHVRDVSPEELQKAARDACG"
BASE COUNT          289 a          536 c          431 g          293 t
ORIGIN      
        1 gacacgatcg tgccaccgtc cgcatagtcg cccgttaact cagcagccga gctctcgctc
       61 gccgccgcga tcgccatcaa tccaactgca ccacacagcc attgaaacga tcgccgattt
      121 gaaaacaaat ttctcatgcg aatccggggt ttgctgaccg ctgagctttc tgacgcgtcg
      181 acgatgacgt tttcaccgtc gtgttcgatt caattttcgg cgcctcactt cactccggcg
      241 catgcccctg cacgccttcc agcactgggc tcgcatcgac aaccggacgc acttccacgg
      301 tcaacccgat cggcaagccg gggcactcct tcgcgatcgc cgtcgcctca tcgatgtcgg
      361 ccacggtgag aaacaaatat cccgccacga cctccttcgc ctcggcgtaa ggtccgtcgg
      421 tcaccttgct gccgagtgcg ccggagacga ctcgtccacc caatccgagc ggccgtccgt
      481 gctgcacttt ccctttcgac gcgagcccgt cataccaatc gttccattgt cgcgtgagct
      541 gctctttctg ctccgcggtg agatgcgcgt gcgcttcggt gccgccgttg cggaaaagca
      601 gcatgaaggc ggatgttgtc gatgttgttg ccatgcgcat cacacatgtc ggcgcgcgag
      661 cccggcgtca aaactccaga agcgacagaa aatgtcgtcc acttgtccga actctttggt
      721 caggattcat cgagccgcga aagattttga acagcggcgc gctccgtgcg tccgacccgt
      781 attgttcacc caactcccag agccgctatg agcatccctc ccgaatcccc ctcgccgagc
      841 acagatccga agaaggccgc acgcgatcgc atgaatacaa tcactccgca cctggtctgc
      901 gccggcgccg ccgatgcgat cgagttctac aagaaagcgt tcggcgcgac cgaggtcacg
      961 cgcgtgccgg ggcccgccgg caaattgatc catgcctcca ttcacatcgg cgattcgacc
     1021 gtaatgctct gcgacgagtt tcccgattgg ggcaacgtca gcccgaagtc gctcaaaggc
     1081 acgcccgtga ccatccacat cacgacggat aacgccgatg cgctcgccgc gcgcgcggca
     1141 gaggcgggtg cgacaatcgt gatgcccgtc gaagacgcgt tctggggcga tcgctacggc
     1201 gtgctggaag atcccttcgg ccaccgttgg tccgtcgcga cacacgttcg cgacgtctca
     1261 ccggaagagc tacagaaagc cgcccgggac gcttgcggtt gaacaagtgc gtctgcgcgt
     1321 gcctggagtt gtttccgtcc aggccactca ctctggctcg cgcgccgcag ttgtccgtgc
     1381 ctccggcctt gcgcagacgc tgcgcccgcg ccatcaccag cgtatgaaga cgctcgcccg
     1441 cggcacccgt cagcgcttgg aaaaaaccgc gcgtcgggct gccaaagccg cctacgcgcc
     1501 gtattcaaaa tttcgagtcg gcgcggcggt gctcacgcgc actggcagg
//
LOCUS       DASPLT010000491         1383 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_6320, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000491 DASPLT010000000
VERSION     DASPLT010000491.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1383)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1383)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1383
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6320"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..350)
                     /locus_tag="VEA63_05065"
     CDS             complement(<1..350)
                     /locus_tag="VEA63_05065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD83394.1"
                     /translation="MSLLSLISKSGVIAALLAFATAANAASSSAQAWLENYYQQPAPE
                     RFASAIYELSRSGYFEQEGRVPLAIGFIASVFQQNPDRVETWMNVNRVLPVAHQRILV
                     SALWYSGHAKGADYL"
     gene            641..>1383
                     /locus_tag="VEA63_05070"
     CDS             641..>1383
                     /locus_tag="VEA63_05070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009509796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetamidase/formamidase family protein"
                     /protein_id="HYD83395.1"
                     /translation="MNKPPVSRIHFSPTQKIAFACGLMAAALAPATIRAMEIPYEYHV
                     RCTPENAIFGYFSATKKPVLTVKSGAVVRIDGGGGNKWGDAESPNEWLKQNDIPLTVE
                     SSPELSEIMHAIKESPNRLPPPPGSPPGTRTGGHFLIGPIYIEDAEPGDSLEVRILDV
                     TPRLPYGANGARPGGGGLPDLVPRPYGKAYKLDLKRNAAVFSENIEVPLAPFNGVNGT
                     CPPDSEGPNRRSGPPGLFAGNLDSKDLVAG"
BASE COUNT          268 a          377 c          492 g          246 t
ORIGIN      
        1 aggtaatcgg cgcccttggc gtgaccggaa taccaaagcg cggacacgag gatgcgctgg
       61 tgagcgacag ggaggacgcg gttgacgttc atccaggttt cgacgcgatc gggattctgt
      121 tggaagaccg aagcgatgaa gccgatcgcg agcgggacgc ggccttcctg ttcgaagtag
      181 ccggagcggc tgagctcgta gatcgcggag gcgaagcgct cgggcgcggg ctgttgatag
      241 taattttcga gccaagcctg ggcggagctt gaagcggcgt tcgcggcggt cgcgaacgcg
      301 agaagagcgg cgatgacgcc gcttttggag atgaggctaa gtaatgacat gacggatacg
      361 gtttcggggt tgacgtggcc cggggtgggg cgaggaccaa aacctactcc cggcttcggc
      421 gacgagtaaa gcggggcaac gatgaaatga tttcatgttt ttgggacgta gatcgcagtg
      481 aagcgcgcgc gatgcgatga gagccgtttg ggcgatcctc atgggatcgc gccaaaaacg
      541 ggcggcggca tcgatgaatt ttttcgatgc gccgcgagtg gtggggcgag gccgttggca
      601 tgtgctgcgc tgacgggcgt gcggctcgtt gcccacacac atgaacaaac cgcctgtctc
      661 acgtattcac ttttcaccga cacaaaagat cgccttcgcc tgcgggctga tggcggccgc
      721 gctcgcgccg gcgacgatcc gagcgatgga gattccttac gagtatcacg tgcgctgcac
      781 gccggagaac gcgattttcg gttatttctc ggcgacgaag aaaccggtgt tgacggtgaa
      841 atcaggtgcg gtggtccgga tcgacggcgg tggcggaaac aagtggggcg atgccgagag
      901 tccgaacgag tggctgaagc agaacgacat tccgttgacg gtcgagagca gcccggagct
      961 cagcgaaatc atgcacgcga tcaaggagtc gccgaatcga ctgccgccgc cgccggggag
     1021 tccgccgggc acgcgcacgg gcgggcattt tctgatcggg ccgatttaca tcgaggatgc
     1081 ggagcccggc gactcgttgg aggtgcgtat tctcgatgtc acgccgcggc tgccgtatgg
     1141 cgccaacggc gcgcgtccgg gcggcggcgg tttaccggat ctcgtgccac ggccttacgg
     1201 caaggcctac aagctcgacc tcaagcgcaa cgccgcggtg ttcagcgaaa acatcgaagt
     1261 gccgctcgcg ccgttcaacg gcgtcaacgg cacgtgtccg ccggattcgg aagggccgaa
     1321 tcgtcgctcg ggccctccgg gcctttttgc gggcaacctc gacagcaagg atctcgtggc
     1381 ggg
//
LOCUS       DASPLT010000492         1129 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_6323, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000492 DASPLT010000000
VERSION     DASPLT010000492.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1129)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1129)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1129
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6323"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            14..>1129
                     /locus_tag="VEA63_05075"
     CDS             14..>1129
                     /locus_tag="VEA63_05075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD83396.1"
                     /translation="MSGTFTIAPTDKSGRDFRAKGWLGYVGKHHLRFAGSGEYFLKAG
                     PDSPETLLAYSDFDDTTSEKKDVPLKTWQPHIRDWREGDPTWANGKGKGLIGALNYLA
                     SEEMNTVSFLPYNAGGDGDNVWPFTARGDKLHYDCSKLDQWGIVFDHAQANGIHLHFK
                     LQENEMDDDRVGTARELKDVPESLDNGALGVERKLYLRELIARFGHLLAVTWNLGEEN
                     TQTPEEQRAMLQFIRDTHYWPSNIVAHTFTNEQELVYLPLMGEQSALTGLSLQNDWAE
                     SHLWTVKWVRESARTGRPWIVAHDEQGHWAYGVPPDPGVEGFSGTADDYRRRYGLHDV
                     RKLTLWGNLMGGGAGVEYYFGYRLPQHDLNVEDFRSRD"
BASE COUNT          232 a          289 c          401 g          207 t
ORIGIN      
        1 gccgtttgat ggggtgagcg gaacgttcac gatcgcacca accgacaaga gcgggcgcga
       61 ctttcgtgcg aagggctggc tcggttacgt cggaaaacat cacctgcgct tcgccggcag
      121 cggcgagtat tttctgaaag cgggaccgga ttcgccggag acgctgctgg cgtattcgga
      181 tttcgacgac accacgtcgg agaaaaagga tgtgccgtta aagacgtggc agccgcacat
      241 ccgcgactgg cgtgagggcg atccgacgtg ggcgaacgga aagggcaagg ggttgatcgg
      301 cgcgctcaac tatctcgcga gcgaggaaat gaacacggtg tcgtttctgc cttacaacgc
      361 gggcggtgac ggcgacaacg tgtggccgtt caccgcgcgc ggcgacaagc tgcactacga
      421 ctgctcgaag ctcgatcagt ggggaatcgt gttcgatcac gcgcaggcga acgggattca
      481 tctgcatttc aaacttcagg aaaacgaaat ggacgacgat cgtgtcggca cggcgcgcga
      541 gttgaaggac gtgccggagt cgctcgacaa cggcgctctg ggcgtggagc ggaaactcta
      601 cctgcgtgaa ctcatcgccc gcttcgggca tctgctcgcg gtgacgtgga atctcggcga
      661 ggaaaacacg caaacgccgg aggagcagcg ggcgatgctg cagttcatcc gggacacgca
      721 ttattggccg agcaacatcg tggcgcacac gttcacgaac gagcaggagt tggtttacct
      781 gccgttgatg ggcgagcagt cggcgctgac gggactctcg ttgcagaacg attgggcgga
      841 gtcgcatctg tggacggtaa aatgggtgcg ggagtcggcg cggacggggc ggccgtggat
      901 cgttgcgcat gacgagcaag gtcactgggc atatggcgtg ccgcctgatc cgggcgtcga
      961 aggttttagc ggcaccgccg acgattaccg gcggcgttat ggcttgcatg acgtgcgcaa
     1021 gctcacgctg tgggggaatc tcatgggcgg aggcgcggga gtggagtatt attttggata
     1081 caggctgccg cagcacgatt tgaacgtgga ggattttcgg agtcgcgat
//
LOCUS       DASPLT010000493         1421 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_211029, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000493 DASPLT010000000
VERSION     DASPLT010000493.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1421)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1421)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1421
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_211029"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1421
                     /locus_tag="VEA63_05080"
     CDS             <1..>1421
                     /locus_tag="VEA63_05080"
                     /inference="COORDINATES: protein motif:HMM:TIGR02604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PVC-type heme-binding CxxCH protein"
                     /protein_id="HYD83397.1"
                     /translation="DGDGRFNTSKVFYDQLNFGSGIAVGHGGVWVGSPPYLLFIPDKN
                     RDDKPDGPPQPVLDGWGHHDTHETLNSFVWGPDGWLYGNQGVYTHSNVGRPDARDHQR
                     IPLDACVWRYHPTKKIFEVFAEGMSNQWGLDFNDVGDAFVTACVIPHLYHVIQGGAYK
                     RQSGPHPNPHVYEDIQTIADHFHYDRGVKWTDSRFQAGGTDALGGGHAHAGTLIYLGD
                     NFPAEYRGTLFTHNVLGSRINRDILEPSGSGYIGKHAPDFMKANDGWFRGLRLEIGPD
                     GSLFNSDWYDARACHQQRPQDRTNGRIYKISYGQPKPVRVDLSKYSSAELVELQLHDN
                     EWFVRRARVILQERGADRAVHAALTKLVREQKEIRKRLRALWALHVTQGLTESLALEL
                     LKDSEPHLRGWTIQLVCEDKAPSAALLAAFLDLAKNDPSPVVRRFLASGAQRIPLVKR
                     WDIVEALLAHGEDANDPSLPLLY"
BASE COUNT          289 a          482 c          391 g          259 t
ORIGIN      
        1 ccgatggcga cggtcgcttc aacacaagca aggttttcta cgaccaactc aacttcggca
       61 gcggtatcgc cgtcggtcac ggcggcgtgt gggtcggcag tccgccgtat ctgttgttca
      121 tccccgataa aaatcgcgac gacaaacccg acggcccgcc gcaacccgtg ctcgacgggt
      181 ggggccatca cgacacgcac gaaacactaa acagcttcgt ctggggtccc gacggctggc
      241 tctacggcaa ccaaggggta tatacacatt cgaacgtcgg ccgccccgac gcgcgcgatc
      301 atcaacgcat cccgctcgac gcctgcgtgt ggcgctatca ccccacgaag aaaatcttcg
      361 aagttttcgc ggaaggcatg agcaatcagt ggggcctcga cttcaacgac gtcggcgacg
      421 cgttcgtcac ggcatgcgtc atcccacatt tgtatcacgt gattcagggc ggcgcctaca
      481 aacgccagag cgggccgcat ccgaatccgc acgtttacga agacatccaa acgattgccg
      541 accacttcca ctacgatcgc ggcgtgaaat ggaccgattc ccgtttccaa gcaggtggca
      601 ccgatgcctt gggtggcggt cacgcgcatg caggcacgtt gatttacctc ggcgacaatt
      661 tccccgccga ataccgcggc acgcttttca cgcacaacgt cctcggcagc cgcatcaatc
      721 gcgacatcct cgagccctcc ggctccggct acatcgggaa acacgcgccc gatttcatga
      781 aggccaacga cggctggttc cgcggcctgc gcctcgagat cggccccgat ggctccttgt
      841 tcaactccga ctggtatgac gcccgcgcct gtcatcaaca gcgtccgcag gatcgaacca
      901 acggccgcat ctacaaaatc tcctacggcc aaccaaagcc cgtccgggtc gatctctcca
      961 agtattccag cgccgaactt gtcgagctcc agctccacga caacgagtgg ttcgtccgcc
     1021 gcgcccgcgt gatcctgcag gagcgtggcg ccgaccgggc ggtgcatgcc gcgctcacga
     1081 aactcgttcg cgagcagaaa gagatccgta aacgcctccg tgcgctctgg gcgctgcacg
     1141 tcacgcaagg actcacggaa tcgctcgcac tcgaattgtt gaaagattcc gagccgcact
     1201 tgcgcgggtg gacgatccag ctcgtatgcg aagacaaagc gccgagcgct gctttgctcg
     1261 cggcgtttct cgacctcgcg aaaaacgatc cttcgcccgt cgtgcgccga tttctcgcct
     1321 ccggtgcgca acgaattccc ttggtgaaac gttgggacat cgtcgaggcg ctgctcgcgc
     1381 atggtgagga tgccaacgat ccgagcctgc ccctgctcta c
//
LOCUS       DASPLT010000494         1122 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_230496, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000494 DASPLT010000000
VERSION     DASPLT010000494.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1122)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1122)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1122
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_230496"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..224
                     /locus_tag="VEA63_05085"
     CDS             <1..224
                     /locus_tag="VEA63_05085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD83398.1"
                     /translation="SEMESRAQPKALDDSERTPAEASADQFRRSAIVGGPAAESRSSA
                     TNRFTSIEGTVRVGAATNEQRFKAVVSGR"
     gene            243..1025
                     /locus_tag="VEA63_05090"
     CDS             243..1025
                     /locus_tag="VEA63_05090"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: protein motif:HMM:NF000594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HYD83399.1"
                     /translation="MIDANLLPAVFIVARVLAGDKVAPMLKPPGKSRFRYERELAQRG
                     FATIAGVDEAGRGPLAGPVVAAAVIFPLRWIVEGLPHAFRGLNDSKQLTPEQREAYFA
                     RLVGHAEVRYGLGWSDNRLIDELNILQATFRAMNDAVAKLNPAPAHVLVDGNQRFSVH
                     WPQTTIVDGDARSYSIAAASVLAKVTRDRMMVEFDRQWPQYGFSEHKGYSTEKHLAAL
                     AAHGPCPLHRRSFAPIRPEAPTLPELFGSRAIDQPPRPAASA"
BASE COUNT          203 a          345 c          363 g          211 t
ORIGIN      
        1 ggtcggaaat ggaatcccgc gcccagccga aggcactcga cgattccgag cgcacgcctg
       61 cggaagcctc cgccgaccaa tttcgccgga gtgcgatcgt gggcggccct gcggcggagt
      121 cccgctcctc cgccaccaat cgattcactt caatcgaagg caccgtgcgc gttggcgccg
      181 ctacgaacga acagcggttt aaagccgtgg tcagcgggcg ctgagttggc attctgcgcg
      241 acgtgatcga cgcgaatttg cttcccgccg ttttcattgt tgcccgcgtg ttggcgggcg
      301 ataaggtcgc gccgatgctg aagcctcccg gcaaatcccg tttccgctac gagcgcgaac
      361 ttgcacaacg gggctttgcc accatcgccg gggtggacga agccggtcgc ggaccattag
      421 cgggaccagt ggtcgccgca gccgtcattt ttccgctgcg ctggattgtc gagggcctgc
      481 cgcacgcttt ccggggcctg aatgactcga agcaacttac gcccgagcaa cgtgaagctt
      541 attttgcgcg cctggtcgga catgcggagg tccgctacgg cttgggatgg agcgacaatc
      601 ggctcattga cgaattgaac attcttcagg cgacgttccg cgccatgaac gacgcggtgg
      661 caaagctgaa cccggcgccg gcgcatgtgc tcgtcgatgg caaccagcgt ttcagcgtgc
      721 actggccgca gacaacgatc gttgacggcg acgcgaggag ctattcgatc gctgccgcga
      781 gcgtgctggc gaaggtgacg cgggatcgga tgatggtgga gttcgatcgc cagtggccgc
      841 agtacggatt ttccgagcac aagggttaca gcacggagaa gcatctggcc gcgctggccg
      901 cgcacgggcc ttgtccgctg caccgccgca gcttcgcgcc gatccggccg gaggcgccga
      961 cgttgcccga gttgtttgga tccagggcga ttgatcaacc gccacggccg gcggcttcag
     1021 cataacgaaa aagggccgcc catgatggac ggcccaatga gagatcacgc ctgacgctat
     1081 tttgtgctgc ccttgtggtt gcgcacaaac tcgatgcatt tc
//
LOCUS       DASPLT010000495         2560 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_230512, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000495 DASPLT010000000
VERSION     DASPLT010000495.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 2560)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2560)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2560
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_230512"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..922
                     /gene="tdh"
                     /locus_tag="VEA63_05095"
     CDS             <1..922
                     /gene="tdh"
                     /locus_tag="VEA63_05095"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008743 - L-threonine 3-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HYD83400.1"
                     /translation="ICGTDVHIHNWDTWAAKTIRPPLVIGHEFVGVIEEVGANVQGYA
                     PGDLVDGEGHIVCGRCRNCLAGRRHLCKDTKGVGVNRDGAFAEYLCIPASNAVHVDPA
                     IPLEVLSCFDPLGNATHTALQYDLVGEDVLITGAGPIGCMAAAIARRAGARKVVVTDI
                     NADRLALAKRMGATRVANVAVETLNDVQREIGMTEGFDVGLEMSGSPRALNDMIDNMA
                     HGGRIALLGIMPDKAEIDWNKVVFNMLTIRGVYGREMYETWYKMTAMIQSGLDISPVI
                     THRFHFTEFARGFELMRAGKCGKVILDWEA"
     gene            1163..2353
                     /gene="kbl"
                     /locus_tag="VEA63_05100"
     CDS             1163..2353
                     /gene="kbl"
                     /locus_tag="VEA63_05100"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HYD83401.1"
                     /translation="MTDSFSAHVRKILGEIEGAGLLKRERVIATPQSAHIRTAEGKQV
                     LNFCANNYLGLADHPDVVAAAHAALDRWGYGMSSVRFICGTQEIHKEVEARLSAFLGT
                     EDTILYSSCFDANAGIFETLLSSEDAVISDALNHASIIDGIRLSKAVRYRYANSDMNE
                     LEARLQEAERANARFKLIATDGVFSMDGYVANMKAICALAEKYGALVMMDDSHAVGFV
                     GKTGRGTHEHCEVMGRVDVISGTLGKALGGASGGYVSGRKEIVELLRQRSRPYLFSNT
                     LAPMIAAASIAALDLVEKSTELRDRLAENTAFFRAGLKAAGLTIREGFHPIVPVMLGD
                     AALSQKFAARMLERGVYVVGFFYPVVPEGAARIRTQVSAAHTRGDLEKAIEAFASVKR
                     ELGV"
BASE COUNT          479 a          550 c         1008 g          523 t
ORIGIN      
        1 gatctgcggg acggacgtgc atatccacaa ctgggatacg tgggcggcga agacgatccg
       61 gccgccgctg gtgatcgggc acgagtttgt cggcgtgatc gaggaagtcg gcgcgaatgt
      121 gcaaggctat gcgccgggcg acctcgtcga tggcgaaggc catatcgtgt gcgggcggtg
      181 tcgcaactgc ctcgcggggc ggcggcattt atgcaaggac acgaaaggcg tcggcgtgaa
      241 ccgcgacggg gcgtttgcgg agtatttgtg cattccggcg agcaatgcgg tgcatgtgga
      301 tccggcgatt ccgctggagg tgctgtcgtg tttcgatccg ttgggaaacg cgacgcatac
      361 ggcgttgcag tatgatttgg tcggcgagga cgtgttgatc accggcgcgg ggccgatcgg
      421 ctgcatggcg gcggcgattg cgcgccgggc gggggcacgg aaggtggtgg tcacggacat
      481 caacgcggat cgcttggcgc tcgcgaagcg gatgggcgcg acgcgcgtgg ctaatgttgc
      541 ggtggagacg ttgaacgacg tgcagcgcga gattgggatg acggaaggat tcgacgtggg
      601 attggagatg tcggggagtc cgcgggcctt gaacgatatg atcgacaaca tggctcatgg
      661 cgggcggatc gcgttgctgg ggatcatgcc ggacaaggcg gagatcgatt ggaacaaagt
      721 ggttttcaac atgctcacga ttcgcggcgt gtatgggcgc gagatgtatg agacgtggta
      781 taagatgacg gcgatgattc agagcgggct ggatatctcg ccggtgatca cgcatcggtt
      841 tcacttcacg gagttcgcgc gcgggtttga gctgatgcgt gcggggaagt gcgggaaggt
      901 gattttggat tgggaggcgt gaagcggagg agatgatgaa ccacgaaata cccgagaggg
      961 aaacggcggt atttaaccac gaatggacac gaatgaacac gaacggggtg gagaccgaac
     1021 gtgtagcggg aaacgagtcg ccgtttggtg gtcgtccgac cggctcagat gctgcggctc
     1081 ggcttcttgg gcattcgtgt caattcgtgt ccattcgtgg tttttcagtg catgcggcta
     1141 tgcttgaacg gagatctgcg agatgacgga ttcgttttca gcgcatgtgc ggaagatcct
     1201 cggggagatc gagggtgcgg ggttgctcaa gcgcgagcgc gtgatcgcga cgccgcagag
     1261 cgcgcacatt cggacggcgg agggaaagca ggtgctgaat ttttgcgcga acaactatct
     1321 cgggttggcg gatcatccgg acgtcgtcgc ggcagcgcat gccgcgctcg atcggtgggg
     1381 ttacgggatg tcgtcggtgc ggttcatctg cgggacgcag gagatccaca aggaagtgga
     1441 ggcgcggttg agcgcgtttc tcgggacgga ggatacgatt ctgtattcgt cgtgcttcga
     1501 tgcgaacgca gggattttcg agacgttgtt gtcgagcgag gatgcggtga tcagcgatgc
     1561 tttgaatcat gcgtcgatca tcgatggcat ccggctctcg aaggcggtgc gttaccgtta
     1621 tgcgaacagc gacatgaacg agttggaggc gcgattgcag gaggcggagc gggcgaatgc
     1681 gcggttcaag ctgatcgcga cggatggggt tttttcgatg gacggttatg tggcgaacat
     1741 gaaggcgatc tgtgcgctgg ccgaaaaata cggagcgctg gtgatgatgg acgacagtca
     1801 cgcggtcggc ttcgtgggga aaacggggcg gggaacacac gagcattgcg aggtgatggg
     1861 gcgcgtggac gtgatttctg ggaccttggg aaaggcgttg ggcggggcga gtggcggtta
     1921 tgtgagcggg cggaaggaga tcgtggaatt gctgcggcag cgatcgaggc cgtatctgtt
     1981 ttcaaatacg ctggcgccga tgatcgcggc ggcgtcgatc gcggcgttgg atttggtgga
     2041 gaagtcgacg gagctgcgcg accggctggc ggagaacacg gcgtttttca gggcaggttt
     2101 gaaagcggcg ggactgacga ttcgcgaagg ctttcatccg atcgtgccgg tgatgttggg
     2161 cgacgcggcg ctgtcgcaga agttcgccgc gcggatgttg gaacgcggcg tgtatgtggt
     2221 gggcttcttt tatccggtgg tgccggaagg ggcggcgcgg attcgcacgc aggtgagcgc
     2281 ggcgcacacg cgcggggatt tggagaaagc gatcgaggcg ttcgcgagcg tgaaaaggga
     2341 attgggggtg tgagcgcggt gcgggatgcg cgggggagga cggcggattt tttttaacca
     2401 cgaatggaca cgaagggaca cgaattcgga gccggacggc ggatggggcg gtgttttcga
     2461 tttgggtggt ttaggtgcgt tcgaataggg cttttttttg gccacggact acctggaatg
     2521 agtgacagga ttttgggcgg ctggagcgcg gcgggtggga
//
LOCUS       DASPLT010000496         1140 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_191100, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000496 DASPLT010000000
VERSION     DASPLT010000496.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1140)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1140)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1140
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_191100"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..807)
                     /locus_tag="VEA63_05105"
     CDS             complement(<1..807)
                     /locus_tag="VEA63_05105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HYD83402.1"
                     /translation="MNQNIRNIAIIAHVDHGKTTLVDKLLKEGGVFRANQQVEERAMD
                     SMDLEREKGITIKAKNTSVHWGDKIINILDTPGHADFGGEVERALRMVDGVLLVIDAY
                     DGPQAQTRFVLRKALGHGLKVVIVINKIDRDNADPAKMYDKVLELLMELNATEEQFDA
                     PVVYGSGRDGYMMYHLGEEKKNMTPLFQTIIDHVPPPFAKPEEPFHMLVSNIDWSDYV
                     GRIAVGKILGGTIKVGENVFVVRHADGKRVRAKITKIFEFTGLGQREVESA"
     gene            complement(956..>1140)
                     /locus_tag="VEA63_05110"
     CDS             complement(956..>1140)
                     /locus_tag="VEA63_05110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HYD83403.1"
                     /translation="ADRGTGLGLATVLRLVKRHDGFVTVETAVGAGSCFTCYFPAHHA
                     TPAAPNTVGAAADAVA"
BASE COUNT          203 a          334 c          378 g          225 t
ORIGIN      
        1 cgcggattcg acctcgcgct ggccgaggcc ggtgaactcg aaaatcttgg tgatcttggc
       61 gcgcacgcgc ttgccgtcag cgtgacggac aacgaagacg ttttcgccga ccttgatcgt
      121 gccgccgaga atcttgccga cggcgatacg gccgacgtag tcgctccaat caatgttcga
      181 cacgagcatg tggaacggct cctcgggctt cgcgaacggc ggcgggacgt ggtcgataat
      241 cgtctggaag agaggcgtca tgttcttctt ctcttcgccg aggtgataca tcatgtagcc
      301 gtcgcggccg gaaccgtaga cgacgggcgc gtcgaactgc tcttcggtgg cgttgagctc
      361 catgagcagc tcgagcacct tgtcatacat cttggcggga tcggcgttgt cgcggtcgat
      421 cttgttgatg acgatcacga ccttgagccc gtggcccaac gccttgcgga gaacgaagcg
      481 ggtttgcgcc tgcgggccgt cgtaggcatc gatgacaagc aaaacgccgt cgaccatgcg
      541 gagggcgcgt tcgacctcgc cgccgaagtc ggcgtggccg ggcgtatcga gaatgttgat
      601 gatcttgtcg ccccagtgga cggacgtgtt cttcgccttg atcgtgatgc ctttttcgcg
      661 ctcgaggtcc atcgagtcca tggcgcgctc ctcgacctgc tgattggcgc ggaaaacgcc
      721 gccttcttta aggagtttgt cgacgagcgt ggttttgccg tggtcgacgt gggcaataat
      781 ggcgatgtta cggatgttct gattcatgga gcggacgcgt tcattctttg gaaaaagggg
      841 aagaacgtgc gggtcggccc gcgcgaacgc aaggccgaag ccacggcgga ctcaggacgc
      901 taatttaccc cagcggcgcg ccggccgcaa tcgccggcaa cgccgccatc cgccctcaag
      961 cgacggcgtc ggctgcggct ccgacggtgt tcggagctgc aggtgtcgcg tgatgcgccg
     1021 ggaaataaca cgtgaagcaa ctgcccgcgc cgacagcggt ttccacggtg acgaagccgt
     1081 cgtgacgttt cacgaggcgc agcacggtcg caagaccgag cccggtgccg cgatcggccg
//
LOCUS       DASPLT010000497         1011 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_271886, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000497 DASPLT010000000
VERSION     DASPLT010000497.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1011)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1011)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1011
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_271886"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            285..656
                     /locus_tag="VEA63_05115"
     CDS             285..656
                     /locus_tag="VEA63_05115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HYD83404.1"
                     /translation="MSNSKLYVGNLSFKTTEDELRSAFGQFGSVTDVYVAMDKMTGRP
                     RGFAFVTMGTADEAKSAAEKLNGTDLGGRQLTVNEARPKEERPGGGFGGGGGGRGFGG
                     GGGGRGGFGGGRDRDRGERRY"
     gene            844..>1011
                     /locus_tag="VEA63_05120"
     CDS             844..>1011
                     /locus_tag="VEA63_05120"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HYD83405.1"
                     /translation="MPFKALNLSEPIFRGIQAAGYTDPTPIQLRAIPVILSGGDLIAS
                     AQTGTGKTAAFA"
BASE COUNT          183 a          328 c          283 g          217 t
ORIGIN      
        1 cggtcgctcc gccctgagct cgggcacgga gcacgtatcc agttcccaac tgcatgccac
       61 ggtgcacctt cgcccgcgtt tttcaccgtt ccgccacgat gacaaatcgc gaattccctt
      121 gcatttatcc ccctcccgtg cgttcttccg gcacatcgtt agagaaagcg ttagatgaca
      181 gtatctggtg agtcgtaggc tgagcgatag gcttaagtga tgatttcgaa acccatggtg
      241 ggaacggact ggcagcagac gatggatcta cgagacatca ctaaatgagt aactccaagc
      301 tatacgttgg aaacctgtcg ttcaaaacca ccgaagacga gctccgctcc gcgttcggcc
      361 agttcggttc cgtgaccgac gtctacgttg ccatggataa gatgaccggc cgcccgcgcg
      421 gtttcgcttt cgtcaccatg ggcacggccg atgaggccaa gtccgcggcc gaaaagctca
      481 acggcaccga tctcggtggc cgtcagctca ccgttaatga agctcgtccg aaagaagagc
      541 gcccgggtgg cggcttcggc ggcggcggcg gtggccgtgg tttcggcggc ggcggcggtg
      601 gccgcggcgg cttcggcggc ggtcgcgatc gcgatcgcgg cgagcgccgt tactaatccg
      661 tttcgcttct aacgaaccgg ttttctgcag gacggagctc ctttgcgggc tccgtccttt
      721 ttctttggcc cggtcggcaa cgtccgcgca ctccgatttg tcctttgctt cgccatcctg
      781 agcgaacgcg aaggatctct attattttct gctgcaccat tccgcatcct gcgcccgacc
      841 tgcatgcctt tcaaagcact caatctctcc gaaccgattt tccgcggcat tcaagccgcc
      901 ggctacaccg atcccacgcc gatccaactg cgtgcgattc cggtcattct cagcggaggc
      961 gatctgatcg cctccgcgca aacgggcacc ggcaaaaccg ccgccttcgc c
//
LOCUS       DASPLT010000498         1132 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_129063, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000498 DASPLT010000000
VERSION     DASPLT010000498.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1132)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1132)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1132
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_129063"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..525
                     /locus_tag="VEA63_05125"
     CDS             <1..525
                     /locus_tag="VEA63_05125"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012377092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HYD83406.1"
                     /translation="LRNNNTRLLTKLGADTGFDSIGDFSQARALSLYLDALDSTDELP
                     RTVLYNVNPKDNYTFGTMIGNFQDGSVPGKIQFGSGWWFLDQKEGMEWQINALSNLGL
                     LSRFVGMLTDSRSFLSYPRHEYFRRVLCNLLGADMERGEIPHDRELVGGMIRRICFAN
                     ARDYFRLELDPSFR"
     gene            629..>1132
                     /locus_tag="VEA63_05130"
     CDS             629..>1132
                     /locus_tag="VEA63_05130"
                     /inference="COORDINATES: protein motif:HMM:NF024295.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 44 protein"
                     /protein_id="HYD83407.1"
                     /translation="MNFLSRARRQLLPASVLLLSANLRAADSVQVFIDFMPTPAEPKP
                     ISPYIYGSNAALPGVTFPLLRQGGNRMTGYNWENNASNAGHDYRHQNDNFLTWVAGLP
                     DTLADTTPGLVLTHFHDAALDAGTSYSILTLPMAGYVAADKSGPVTAAQAAPSARWLP
                     VANQKPAT"
BASE COUNT          201 a          432 c          288 g          211 t
ORIGIN      
        1 ctgcgcaaca acaacacccg cctgctcacg aaactcggcg ccgacacggg cttcgactcg
       61 atcggagatt tctcccaagc ccgcgcgctc tccctctatc tcgacgcgct cgactccacc
      121 gacgagcttc cgcgcaccgt cctctacaac gtgaatccaa aggacaacta caccttcggc
      181 acgatgatcg ggaatttcca ggacggctcc gtgcccggta aaattcaatt cggcagcggc
      241 tggtggttcc tcgatcagaa ggaaggcatg gagtggcaga tcaacgcgct ctcgaatctc
      301 ggcctgctca gccggttcgt gggcatgctc accgactcgc gcagcttcct cagttacccg
      361 cggcatgagt attttcgccg cgtcctctgc aatcttctcg gcgcggacat ggagcgcggc
      421 gagatcccgc acgatcgcga acttgtcggc ggcatgatcc gccgcatctg cttcgccaac
      481 gcccgcgatt atttccgcct cgagctcgat ccgagtttcc gttgagcgcc ttccggatgc
      541 gctttaccgc atcccctcgt tcgcttcggc cgctaatccc gttgcgacgg caacacggca
      601 cttcatcatc gcgacaacct tcctcccggt gaatttcctc tcgcgcgccc ggcggcagtt
      661 gcttcccgcc agtgtccttc tcttgagcgc caacctgcgc gcggccgact ccgtgcaggt
      721 cttcatcgac ttcatgccta ccccggcaga gccgaagccg atcagcccct acatctacgg
      781 cagcaacgcg gctctccccg gcgtcacgtt cccgcttctc cggcaaggcg gcaatcgcat
      841 gaccggctac aactgggaaa acaacgcctc gaacgccggc cacgactacc gtcaccaaaa
      901 cgacaacttc ctcacgtggg tcgccggcct tcccgacacg ctcgccgaca ccacccccgg
      961 cctcgtgctc acgcatttcc acgatgccgc cctcgacgcc ggcacgagct actcgattct
     1021 cacccttccc atggcgggct acgtcgccgc cgacaaatcc ggcccggtca ccgccgcgca
     1081 agctgctccg tctgcgcggt ggctgccggt ggcaaaccag aaacccgcga cg
//
LOCUS       DASPLT010000499         1175 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_191163, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000499 DASPLT010000000
VERSION     DASPLT010000499.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1175)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1175)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1175
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_191163"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..291
                     /locus_tag="VEA63_05135"
     CDS             <1..291
                     /locus_tag="VEA63_05135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007360152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twitching motility protein"
                     /protein_id="HYD83408.1"
                     /translation="RGFICQKLIPALEGGGRVPTNEILTADAVVKNLILEGQFEKLQG
                     ILEGTGDSNSFSFNKDIYRLIKAGKISKADGLRFSPNPQALEMNLKGIFVRS"
     gene            294..>1175
                     /locus_tag="VEA63_05140"
     CDS             294..>1175
                     /locus_tag="VEA63_05140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD83409.1"
                     /translation="MSSFREIRGRLAVIAAAACAAANVSEAAKSTDDGIAGAKRDYET
                     ITAPKAGTGPSRLGFPKQSLPELDAAPPLPAGPSPTSPYRKNGITKNDAEEKPEKSEN
                     WLVDAMTKTDQGERDAATDLPNATAKAEAKGDLVALAAALEREEKATEKTREAREELK
                     AAESTTVNPLGAYMTNWLSSRDLALLNPASISTSADGADKMLLAAPGSGGSEASVAAP
                     TAVFDHLAAGNRDGGIDRMVSPTPNPYLAASDDLLIAPPTEFSMPTASAPSAPAPVSP
                     PTTLPAPEAPSRPSPATL"
BASE COUNT          252 a          345 c          382 g          196 t
ORIGIN      
        1 cgcggattca tctgccagaa attgattcca gcgctcgagg gcgggggacg cgtgccgacc
       61 aacgagatcc tcaccgccga cgctgtcgtg aaaaacctga tactcgaggg ccagttcgag
      121 aagcttcagg gcattctcga aggcaccggt gactcgaaca gtttttcttt caacaaggac
      181 atctaccggc tcatcaaggc ggggaaaatc agcaaggccg acggcctgcg tttctcgccg
      241 aatccgcagg cgctggagat gaacttaaaa gggatattcg tcaggtcgtg acaatgagct
      301 cgtttcgcga aatccgcggc cggctggcgg tgatcgccgc cgcggcgtgc gcggcggcaa
      361 acgtgagcga agcggcgaaa tcgacggatg acgggattgc gggcgcgaaa cgcgactacg
      421 aaacaatcac ggccccgaaa gctggcaccg ggccgtcgcg gctcgggttt ccgaaacaga
      481 gtttacctga gttggacgct gcgccgccgt tgccggcagg gccttcgccg acctcgccct
      541 accgcaaaaa cggtatcact aagaacgacg ccgaagagaa gccggagaaa tccgaaaatt
      601 ggttggtcga cgcgatgacg aaaacggacc aaggcgagcg tgacgcggcc actgacctgc
      661 cgaatgcaac cgccaaagct gaagcgaagg gcgatttggt ggcgctcgcc gcggcgctgg
      721 agcgcgaaga aaaagcgacg gaaaaaacac gggaggcgag ggaggagttg aaagcggcgg
      781 agtcgaccac ggtgaatccg ctcggcgctt acatgacgaa ctggctgagt tcgcgcgatc
      841 tcgcgttgtt gaacccggca tcgatctcca cctctgctga tggagcggac aagatgcttt
      901 tggccgcgcc cggcagcggc ggttcggagg caagcgtcgc ggcaccgacc gcagtgttcg
      961 atcacctcgc tgcgggaaat cgcgacggtg gaatcgatcg catggtttcg ccgacgccga
     1021 atccttattt ggccgcgtcg gacgatttgt tgattgcgcc gcccacggaa ttttcgatgc
     1081 cgactgcgtc tgcacctagc gcgccggcgc cggtttcgcc gccgacaact ttgcccgctc
     1141 cagaggctcc gtcacggccg tcgccggcga cgttg
//
LOCUS       DASPLT010000500         2028 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_170759, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000500 DASPLT010000000
VERSION     DASPLT010000500.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 2028)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2028)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2028
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_170759"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            136..>2028
                     /locus_tag="VEA63_05145"
     CDS             136..>2028
                     /locus_tag="VEA63_05145"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HYD83410.1"
                     /translation="MLEDISARKQREIALTKAMQKLQVIADRIPAMLSYVGSDLRYQF
                     VNEGYESLYKRSKEDIIGRRIQEIVAPSVWTVAAPFFERALQGEPISYEGVFAGDNPA
                     FQHSLVSLVPDKGSSDEVRGLFILVTNITELKKTEARLQDAIAGLRASEVNLKALMEA
                     SPDTLLVVDGDGCILYANARVTEMFGYRLEELLGQPVEQLFPPDRREQYKQYCEQVMQ
                     GNVPRGARRPTDLVAVHKNGQPLPVDIGLNPTKFDGKDVLMATVRDIGGIHAAFESSV
                     QIADIVEASGDAIFRCAVDGTVQYWSKGAEKLYGYRADEMLGQRVSRMIPPSDEWSHD
                     EIRGVLERNEIVMRPDSVRLHKDGSPIEVSVAVFPIRDAEGKVIGGASVHRDIREVKR
                     LEAQLRHAQRLDAAGLLAGGVAHDFNNILTVIQGYCSLITEVQPPDSLLTRQVHAIES
                     AAQRASSLTRQLLAFSQKQRRDVKILDPRALLSDLAPILQRAIGEDIRLETDLQSSWN
                     IQEDPADFEQIILNLAVNARHAMPKGGVLRIASSNIETTVFGVPSEMASFSPIPVQPG
                     AYVLLEIVDTGIGIAPDVLTRIFEPFFTTKSRGEGSGLGLPVVYGIIKNIGGGIVVSS
                     REGAGTN"
BASE COUNT          411 a          623 c          644 g          350 t
ORIGIN      
        1 cccggcaaac ttgcgagccc cgatgcgtgg agccgcgtta tcagcgtgga gctgcatctg
       61 cgcacgaaag cgggaaccgg attctggggc cggctgctga gccgcgcgtc gggcgacggc
      121 cagccgattc cgctattgct cgaagacatt tccgcgcgca agcaacgcga aatcgcgctg
      181 acgaaagcga tgcaaaagct gcaggtgatc gccgacagga tcccggcaat gttgtcgtac
      241 gtcggcagcg atctgcgcta tcaattcgtc aatgaaggct acgaaagcct gtacaagcgc
      301 agcaaggaag acatcatcgg caggcgcata caggaaatcg tagcgccatc cgtgtggacg
      361 gtggccgcgc cgttcttcga acgcgcgttg cagggcgagc cgatcagcta cgaaggtgtg
      421 ttcgccggcg acaacccggc gttccagcat tcactcgtca gcctggtgcc ggacaagggt
      481 tcgtccgatg aggtgcgcgg acttttcatt ctcgtcacga acatcaccga actgaagaaa
      541 accgaagcgc gcctgcagga tgcaatcgcg ggtttgcgcg ccagcgaagt gaacctgaag
      601 gccttgatgg aagcgtcgcc cgatacgctg ctcgtcgtcg acggcgacgg ctgcattctc
      661 tacgccaacg cgcgcgtgac ggagatgttc ggttaccgtc tcgaggaact gctcggccag
      721 ccggtggaac aactgttccc gcccgaccgg cgcgaacagt acaagcagta ctgcgaacag
      781 gtcatgcagg gcaatgttcc gcgcggcgcg cggcggccga cggatctcgt tgcggtgcac
      841 aagaatggcc agccgctgcc ggtggacatc ggtctgaacc cgacgaagtt cgacggcaag
      901 gacgtgttga tggctaccgt ccgcgacatc ggcggtattc acgccgcgtt cgaatcgagc
      961 gtgcagattg ccgacatcgt cgaagcatcc ggcgacgcga tcttccgctg cgcggtggat
     1021 ggcacggttc agtactggag caagggcgcc gagaaactgt acggctatcg cgccgacgaa
     1081 atgctcgggc agcgcgtcag caggatgatt cccccttccg acgaatggag ccacgacgaa
     1141 attcgcggcg ttctcgagcg caatgaaatc gtcatgcgcc ctgactcggt gcgcttgcac
     1201 aaggacggat cgccgatcga agtgtccgta gccgtattcc cgatacgcga tgcggaaggc
     1261 aaggtaatcg gcggcgcgtc ggtgcatcgc gacatccgcg aagtgaaacg gctcgaagcg
     1321 cagttgcggc acgcgcagcg gctcgacgcc gcgggcctgc tcgccggcgg cgtcgcgcac
     1381 gactttaaca acattcttac cgtgatccag ggttactgct cgctgatcac tgaagtgcag
     1441 ccgcccgatt cactgctgac gcggcaggtg catgcgatcg aaagcgccgc gcagcgcgca
     1501 agttcgctga cccgccagct gctcgcgttc tcgcagaagc agcggcgcga cgtgaagatt
     1561 ctggacccgc gcgcgctgct cagtgatctc gcgccgatcc tgcagcgcgc aatcggcgaa
     1621 gacattcgcc tcgaaactga cctgcagtcc agctggaaca tccaggaaga tcccgccgac
     1681 ttcgaacaga tcattctgaa cctcgccgtc aatgcgcggc atgcgatgcc caaaggcggc
     1741 gtgttgcgta tcgcgagcag caacatcgaa acgactgttt ttggcgtgcc gagcgagatg
     1801 gcgagtttca gtccgatacc ggtgcagcct ggcgcctacg tgctgctcga aatcgtcgac
     1861 accggcatcg gcattgcgcc cgacgtgctg acgcgcatct ttgaaccctt cttcacgacg
     1921 aaatcgcgcg gcgaaggatc cgggctcggc ctgcccgtcg tgtacggcat catcaagaac
     1981 atcgggggcg gcatcgtcgt gtcgtcgcgc gaaggcgccg gcacgaac
//
LOCUS       DASPLT010000501         2245 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_251977, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000501 DASPLT010000000
VERSION     DASPLT010000501.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 2245)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2245)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2245
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_251977"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1635
                     /locus_tag="VEA63_05150"
     CDS             <1..1635
                     /locus_tag="VEA63_05150"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF012913.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase domain-containing protein"
                     /protein_id="HYD83411.1"
                     /translation="VFAGPGATILNTPALVTSQKAREKYGLPALTDWRGRAVKSDVLR
                     MQRLAKPATVYIEAYSAHPLEKDSSQLYAPPDGYLDSAGNFHPEKQSPGDVPVFEVVL
                     KPEDGLYPLPYMARRADGSAWGHEVGEGPEFRQTFFPDASRLFDEIERWHGSQNANLR
                     SRADFSFFRAVPSGGYRKGLPASARTDAEAGDISPEKSGVDFFPYDNVNHNPPRSSLV
                     RITNLVQSVLADTSYRGAIWLEGSPRAEDTIYWLNLTIGAEAMIVATVAQRPNLIAGS
                     DGAANILDAVDFILSRTWREADGRNRVGAVMIQDQQIFAAREVVKSAARPGGFKATGG
                     HGGIIGSTIGPVLTFVPERRHGKASNVRYALWPAQLAGVRSDGNRIVSAEVKVKDEAG
                     RWMTSAVPYVETLVFPGWMTAEKVGEGAENMVNTTVQRLLAESQLVGLVAEGQTGGYF
                     DGQETAALERAVLRGIPVVSTSRGMSNHHAFVNPTNLIVEGANLPAEKARVLLMACLL
                     RFGNFPPCADPLAPTQAELAALKQRVAQFQEIFSTH"
     gene            complement(1649..>2245)
                     /locus_tag="VEA63_05155"
     CDS             complement(1649..>2245)
                     /locus_tag="VEA63_05155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD83412.1"
                     /translation="LWVYPETLPRLTPPAPLVTAQLTSRLLKQLHAGANVLWLPALDE
                     VHSPVVTGMSSIFWNTAWTENQAPHTLGILCDPSHPLFADFPTDAHSNWQWQELLLGA
                     KPIILDPLPHHYFPLVQLIDTWFENRKLGVVMEARFGRGKLLVCTLNLTAHPERWVAR
                     QFLYSLQRYLSSEQCVPPHAVTLDQIKSVVAAHTREPN"
BASE COUNT          436 a          590 c          778 g          441 t
ORIGIN      
        1 gtgtttgccg ggccgggtgc gacgattctc aatacgcccg cgctggtgac gagccagaag
       61 gcgcgggaga aatatggttt gccggcgttg acggattggc gcgggcgcgc ggtgaagtcg
      121 gatgtgcttc ggatgcagcg gctggcgaaa ccggcgacgg tttatattga ggcttacagc
      181 gcgcacccgc tggaaaaaga ttccagccag ctctatgcgc cgccggatgg ctatctggat
      241 agcgccggca attttcatcc tgagaagcaa tcgcccggcg atgtgccggt gttcgaggtc
      301 gtgctcaaac cggaggatgg actttacccg ttgccttata tggcaaggcg ggccgatggc
      361 agcgcgtggg gccatgaagt cggggagggg cctgagttca ggcagacttt tttcccggac
      421 gcgtcgcgtt tattcgacga gatcgagcgt tggcatggtt cgcaaaacgc gaacctccgg
      481 tcccgcgccg attttagttt tttccgcgca gttccgtcgg gcggatatcg taaaggtttg
      541 ccggcgtcgg cgcgcaccga tgcggaagcg ggcgatatct cgccggaaaa atcgggcgtg
      601 gatttttttc cctacgataa cgtgaatcac aatccgccgc gttcgagctt ggttcgcatc
      661 acgaatctcg tgcaaagcgt gttggcggat acttcctatc gcggcgcgat ctggctcgaa
      721 ggcagcccgc gcgcggaaga cacgatctac tggctgaacc tgacgattgg agccgaggcg
      781 atgatcgtcg cgacggtggc tcagcgtccc aacctcatcg cgggctccga cggtgcggcc
      841 aacatcctgg atgcggttga ttttattttg tcgagaacgt ggcgcgaggc ggacgggcgc
      901 aatcgggtgg gcgcggtgat gatccaggat cagcaaatct tcgcggcgcg cgaagtcgtc
      961 aaaagcgccg cccgcccggg aggcttcaag gcgacgggcg gccatggcgg gatcattggc
     1021 agcaccatcg gtccggtgct gacctttgtc ccggagcggc gtcacggcaa agcttcgaac
     1081 gtgcgttacg cgctgtggcc ggcgcaactc gcgggagtaa ggagcgacgg gaatcgtatc
     1141 gtttcagcgg aagtgaaggt gaaggacgag gcgggtcgct ggatgacttc ggcggtgcct
     1201 tacgttgaaa cgctggtgtt tcccggctgg atgacggccg agaaggtggg cgaaggcgcc
     1261 gagaacatgg tgaatacaac ggtccaacga ctgctggcgg aaagccagtt ggttggactg
     1321 gtggccgaag gacagactgg cggatatttc gatgggcagg aaaccgcggc gctggagcgg
     1381 gcggtgctgc gaggcatccc ggtggtatcg acctcgcgcg gcatgtcgaa tcaccacgcc
     1441 ttcgtcaacc cgacaaacct cattgtcgaa ggcgcgaatc tgccggcgga gaaagcgcgc
     1501 gtgttgctga tggcctgcct gctgcggttc ggaaattttc cgccgtgcgc cgatccgctc
     1561 gctcccacgc aagcggaact cgcggcgcta aaacaacgcg tggcgcagtt tcaggagatt
     1621 ttttccactc actaaacgga ggctattctc agttgggttc gcgggtgtga gcggcgacga
     1681 cgcttttgat ttgatcgagc gtgaccgcgt gcggcggaac gcactgttcg gacgagaggt
     1741 aacgttgcag gctataaagg aactgccgtg cgacccaccg ttccgggtgg gcggtgagat
     1801 tgagtgtgca gacgaggagt tttccccggc cgaaacgcgc ttccatcacg acgccaagct
     1861 ttcgattttc gaaccaggta tcgataagtt ggacgagcgg gaagtagtga tggggaagcg
     1921 gatcgaggat gatcggcttg gcgcctagaa gaagttcctg ccactgccag ttgctgtggg
     1981 cgtccgtggg gaaatcggca aacagcgggt gcgatggatc acacaaaatg ccgagggtgt
     2041 ggggcgcctg attttcggtc caagcggtgt tccaaaaaat cgaggacatg cccgtgacga
     2101 ccggactgtg aacttcatcg agcgccggca gccacaacac atttgcgccc gcgtggagtt
     2161 gcttgaggag tcggctcgtg agttgcgcag tgacgagcgg cgccggcgga gtgagcctgg
     2221 gcaatgtctc cggatagacc cagag
//
LOCUS       DASPLT010000502         1438 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_129110, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000502 DASPLT010000000
VERSION     DASPLT010000502.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1438)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1438)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1438
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_129110"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..311
                     /locus_tag="VEA63_05160"
     CDS             <1..311
                     /locus_tag="VEA63_05160"
                     /inference="COORDINATES: protein motif:HMM:NF025887.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HYD83413.1"
                     /translation="RYAHSDGRVQTKDELLTAVAKNPVSYLSVVPHDVAFQTLAPGAV
                     AMTGRTEIIAQAGRERTRFALRFLSVWREEAGRWRLAAYQSSQLLAPATPAPNSEVAP
                     "
     gene            308..853
                     /locus_tag="VEA63_05165"
     CDS             308..853
                     /locus_tag="VEA63_05165"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0052873 - FMN reductase (NADPH) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HYD83414.1"
                     /translation="MNVLVISASLNSDSNSRLLGREAERALAADGHIATFLDLRDLPL
                     PFCDGEHAYAHENVKRARTALAGADGIILATPIYNYDANAVAKNFIELTGIAWENKVV
                     GFLCASGGPGSYMSIMSLASSLMLDFRCVIVPRFVYAGGAAFVDGKIVDPKIHARVAE
                     CARTTAHLAAAVKTLPQPQKP"
     gene            1087..>1438
                     /locus_tag="VEA63_05170"
     CDS             1087..>1438
                     /locus_tag="VEA63_05170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD83415.1"
                     /translation="MSKRLSSLAWPLLRLAPRRPRRVLALTLALLTFTHLASAAEVQH
                     HGLIFERWICDTFFDGYRPSHYTQQWDIPASINRAHGDVPVNPKAAKYGTPVDLGDAL
                     RQFDIAEPFILAIGY"
BASE COUNT          253 a          560 c          360 g          265 t
ORIGIN      
        1 tccgttacgc gcactccgat ggccgtgtgc aaaccaagga cgagctcctc acggctgtcg
       61 caaaaaatcc cgtcagctac ctctccgtcg tcccccacga tgtcgccttt caaacgctcg
      121 cacccggcgc cgtcgcgatg accggccgca ccgagatcat cgctcaagcc ggacgcgaac
      181 gcacgcgttt cgccctgcgc ttcctctccg tctggcgcga agaagccggt cgctggcgcc
      241 tcgccgctta ccaatcctcg caactcctcg cgcctgccac gcccgccccg aattccgaag
      301 tcgctccatg aacgtgctcg tcatctccgc cagcctgaac tccgacagca acagccgcct
      361 cctcggccgc gaagccgagc gcgcgctcgc cgccgacggc catatcgcca cctttctcga
      421 cctgcgcgat ctgccgcttc cgttctgcga cggcgaacac gcctacgccc acgaaaacgt
      481 gaaacgcgcg cgcaccgcgc tcgccggcgc cgacggcatc attctcgcga cgccgatcta
      541 caactacgac gccaacgccg tcgcaaaaaa ctttatcgag ctcaccggca tcgcttggga
      601 aaacaaagtc gtcggctttc tctgcgcctc cggcggaccc ggcagttaca tgtccatcat
      661 gtcgctcgcg agcagcctca tgctcgattt ccgctgcgtc atcgtgccgc gcttcgtcta
      721 cgccggcggc gccgccttcg tcgacggcaa gatcgtcgac ccaaaaatcc acgcccgcgt
      781 cgccgaatgc gcgcgcacca ccgcccacct cgccgcggcg gtgaaaacgc tccctcaacc
      841 ccagaagccg tagcgcggtc aacctgaccg cgctcgtgag cactctttag taaagtgtcc
      901 cataggtaac ccgaatatat cgagtggcgc ggcagcgccg gtagggacgg gtctccgacc
      961 cgtccgttca cgtcgaaact gcagctcgtt tttctaaaaa gcgctgtaga aaccgcaccg
     1021 cgcctcagga acgcgccccc agctttaccg tcgtcaccca ccgccgcgcg tctcaatctc
     1081 cttcgcgtgt cgaagcgtct ctccagcctc gcttggcctc ttcttcgcct cgcgccgcgt
     1141 cgcccccgcc gcgttctcgc gctcacgctt gccctgctca cctttaccca cctcgcctct
     1201 gccgcggagg tgcagcatca tggcttgatt ttcgaacgct ggatctgcga cacgttcttc
     1261 gacgggtatc gaccctccca ctacacacaa cagtgggata ttcccgcctc catcaatcgc
     1321 gcgcacggcg atgtgcccgt gaatcccaaa gccgcgaaat acggcacgcc tgtcgacctc
     1381 ggtgatgcgc tgcgccagtt cgacatcgcc gaaccgttca tcctcgcgat cggttatt
//
LOCUS       DASPLT010000503         2249 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_6516, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000503 DASPLT010000000
VERSION     DASPLT010000503.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 2249)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2249)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2249
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6516"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(91..1698)
                     /gene="nadB"
                     /locus_tag="VEA63_05175"
     CDS             complement(91..1698)
                     /gene="nadB"
                     /locus_tag="VEA63_05175"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HYD83416.1"
                     /translation="MQIHRTDCLVIGAGLAGSAYALHMARRGFSVELLSLSEPLAANS
                     DWAQGGIIYDTNPDPASLARDIVEASDRTANPAAVEQLVREGPSAVKELLLDDLHVGF
                     DRDAKGALDFTREGGHSERRIIHAKDATGHAILQTVAERVNATPRIARRTGCVAIDLL
                     TLSHNSENPADKYQPLTCFGAYVLNTVTQEVSAIVAKKTVLATGGLGQIFLHSTNQPG
                     TVGHGVAMAYRVGARLVDLEYVQFHPTVFAKKNAPRFLITEALRGEGGVLVNARGERF
                     MDAADPRGSLAPRDVVARAIKQELAASGEPCVFLDVSAMKPEFLRERFPNIYERCFAH
                     GVDITREPIPVVPAAHFACGGVHTDLAGRTSVCHLNAIGETACTGLHGANRLASTSLL
                     ECLTSAKFTAIADAEEIAAAETPEGRSAQAASGYACGPFHLPDPRPWASPTRVADPVL
                     VRQDMLQIQHTMWNYAGVVRSPRRLTRARRILLELREEIQSFYRGCHLTRELIELRNA
                     VQTALLVVHAAALNPRSKGCHYVVDEE"
     gene            complement(1728..>2249)
                     /gene="nadC"
                     /locus_tag="VEA63_05180"
     CDS             complement(1728..>2249)
                     /gene="nadC"
                     /locus_tag="VEA63_05180"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HYD83417.1"
                     /translation="IATQARKFAAAVDGTGVRIVDTRKTTPGWRALEKYAVRCGGCVN
                     HRSSLGEHVLIKDNHIAAAGSLAKAVKLARASAPHLAKIEVEAKTQAEVREALRAGAE
                     VILLDNMTPAQVKAAVGLIGGGAVVEVSGGVRYETLRDYAVDGVDVISIGALTHSAPA
                     ADLSLTVLSGRKK"
BASE COUNT          387 a          815 c          662 g          385 t
ORIGIN      
        1 gcgcaacggg gtgcgacgag cgcaccccct acagtttcga accgggagca ggcaggcacg
       61 gaggcctacc gctacacgaa gcgccgccac tcactcttcg tccacgacgt agtgacatcc
      121 cttgctccgc ggattcaacg ccgccgcgtg cacgaccagc aatgccgtct gcaccgcatt
      181 gcgcagctcg atcaactcgc gcgtgagatg acacccgcga tagaaactct gaatctcctc
      241 gcgcagctcc agcagaattc gtcgtgcgcg cgtgagccgc cgcggcgagc gcacgacgcc
      301 cgcgtaattc cacatcgtgt gctgaatctg cagcatgtcc tgccgcacca gcaccggatc
      361 ggcgacgcgc gtcgggctcg cccatggccg cggatcgggc aggtgaaacg gtccgcacgc
      421 gtaacccgat gcggcttgcg cgctgcgccc ttccggcgtt tccgccgcgg cgatctcctc
      481 cgcgtccgcg atcgcagtga acttcgcact cgtgagacac tcgagcagcg acgtgctcgc
      541 gagccggttc gcgccatgca gccccgtgca cgcggtttcg ccgatcgcgt tgagatggca
      601 cacgctcgtg cgcccggcca ggtccgtgtg cacgccgccg cacgcgaaat gcgccgccgg
      661 caccaccgga atcggctcgc gcgtgatgtc gaccccatgc gcaaagcagc gctcgtagat
      721 gttgggaaat cgctcgcgca aaaattccgg cttcatcgcc gaaacatcga gaaacacgca
      781 cggctctccg ctcgccgcca gctcctgctt gatcgcgcgc gccacgacat cgcgcggcgc
      841 gagcgaaccg cgcggatcgg ctgcgtccat gaaacgctcg ccgcgcgcgt tcaccaacac
      901 gccgccttcg ccgcgcaacg cttccgtgat cagaaagcgc ggcgcgtttt tcttcgcgaa
      961 gacggtcggg tggaactgca cgtattcgag atcaaccaac cgcgcgccga cccgatacgc
     1021 catggccacg ccatgcccga ccgtgcccgg ctgattcgtc gaatgcagaa aaatctgccc
     1081 gagaccgccc gtcgcgagca ccgttttttt cgcgacgatc gccgacactt cctgcgtgac
     1141 ggtgttcagc acgtaggccc cgaagcaggt gagcggttgg tatttgtccg cgggattttc
     1201 ggagttgtgc gacaacgtga gcagatcgat cgccacgcat ccggtccgcc gtgcgatccg
     1261 cggcgtggcg ttcacccgct ccgcgacggt ctggagaatc gcatgcccgg tggcgtcttt
     1321 cgcatgaata atccgccgct cgctgtggcc gccttcccgc gtgaaatcga gcgcgccctt
     1381 cgcatcgcga tcgaagccga cgtgaagatc gtcgagcaat aattccttca ccgccgacgg
     1441 accttcgcgc accaactgct cgaccgccgc cggatttgcc gtgcgatcgc tcgcttcgac
     1501 aatgtcgcgg gcgagcgacg ccggatccgg attcgtgtcg tagatgatcc cgccctgcgc
     1561 ccaatcgcta ttcgccgcga gcggctcgga gagcgacagc agctcgactg aaaagccgcg
     1621 tcgggccatg tgcaatgcat acgccgatcc cgccagtccg gcgccaatca cgagacagtc
     1681 ggtgcggtga atttgcatgc gctaggcgag gccgggtttt aacccgacta ctttttcctg
     1741 ccgctcagca cggttaaact caaatctgcc gccggggccg agtgggtgag cgcgccgatg
     1801 ctgatgacgt ccacgccatc gaccgcataa tcgcgcagcg tctcgtagcg cacgccgccg
     1861 gaaacctcca cgacggcgcc gccaccgatc aagccgaccg ccgccttcac ctgcgcgggc
     1921 gtcatgttgt cgagcaggat cacctccgcg ccggcacgca acgcttcgcg cacctccgcc
     1981 tgcgtcttcg cttccacttc gatcttcgcc aggtgcggcg ccgacgctcg cgcaagcttc
     2041 accgccttcg ccagcgaacc cgccgctgcg atatggttgt ccttgatcag cacgtgctcg
     2101 cccagcgacg agcgatgatt cacgcagccg ccgcagcgca cggcgtattt ttccagcgcc
     2161 cgccagcccg gtgtcgtctt gcgcgtatcc acaatcctga cacccgtgcc atccaccgcc
     2221 gcggcgaact tccgcgcctg cgtcgcgat
//
LOCUS       DASPLT010000504         1565 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_230801, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000504 DASPLT010000000
VERSION     DASPLT010000504.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1565)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1565)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1565
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_230801"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(84..968)
                     /locus_tag="VEA63_05185"
     CDS             complement(84..968)
                     /locus_tag="VEA63_05185"
                     /inference="COORDINATES: protein motif:HMM:NF044415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD83418.1"
                     /translation="MRFGEAAENDSSAAVTIPLRVLGGRAVEQIFDGAREVESKAGVA
                     LYRAGGLLIGHAAEAFVPEELVERTRELYARVIAACEGRPLFRIWNYVPHINAMTGGE
                     ENYRAFCAGRSLAFEAAFGAEFVERLPAASAVGCAGGRLEVVFVAGEEEPRHFENPSQ
                     VPAYRYPAEHGPRSPSFARATVARDGGRELTFISGTAAIKGHETVAPGALDAQLDCTL
                     DNLRLISAAAGLGERLDGRAGSRRHFKIYLRRAADFERVRARLEQTLLTPADAVTYLE
                     ADICRAALDLEIEATVIR"
     gene            1256..>1565
                     /locus_tag="VEA63_05190"
     CDS             1256..>1565
                     /locus_tag="VEA63_05190"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HYD83419.1"
                     /translation="MNTFELSVLFFLQLALVLVVCRSVCWVAVRLGQPPVVGEMIAGV
                     LLGPSLFGYFWPHAQQAMFPEASRPILFSGAQIALALYMFTVGLDFRMDLVHTRLRSA
                     I"
BASE COUNT          268 a          610 c          421 g          266 t
ORIGIN      
        1 acacgcaacg cctccgcgag ctcatcgcct ccgctccgag gtagccgcat cggttacgat
       61 gcggaagcgg gctgaagccc gccctacctg atcaccgtcg cctcgatctc gaggtccagc
      121 gccgcgcgac aaatgtccgc ctcgagatac gtcaccgcat ccgccggcgt aagcagcgtt
      181 tgctcgagcc gtgcacgcac gcgttcgaaa tccgccgcac gccgcaggta aattttaaaa
      241 tgccgccggc tgcccgcgcg cccgtcgagc cgttcgccca gacccgccgc cgccgaaatc
      301 agccgcagat tatccagcgt gcaatcgagc tgcgcgtcca acgcccccgg cgcaaccgtc
      361 tcgtgcccct tgatcgccgc cgtgcccgaa ataaacgtca gctcgcggcc gccatcacgc
      421 gccaccgtag cccgcgcaaa actcggcgaa cgcggaccgt gctccgcggg ataacgatac
      481 gccggcacct gcgacggatt ttcgaaatgc cgcggctctt cctcgcccgc gacaaacacc
      541 acttcgagcc gcccgcccgc gcaacccacc gccgacgccg ccggcagccg ttccacgaac
      601 tccgcgccga acgccgcctc gaaggcaagc gatcgccccg cgcagaacgc gcgataattt
      661 tcttcgccgc ccgtcattgc gttgatgtgc ggcacgtagt tccagatccg aaagagcggc
      721 cgcccttcgc acgccgcgat cacccgcgca tacaactccc gtgttcgctc gacgagttcc
      781 tccggcacaa acgcctccgc cgcgtgcccg atcagcagcc cgcccgcccg ataaagcgcg
      841 acgcccgcct ttgactcgac ctctcgcgca ccgtcgaaaa tctgttccac cgcccttccc
      901 cccagcaccc gcaacgggat cgtcaccgcc gcgctcgaat cgttctccgc cgcttcgccg
      961 aaacgcacgg cgaatccggc cgccttggaa taaatcgtct cgtggtttgc cacaacgcgc
     1021 gctccgttca cccgacccgc gtcgcatccg cggcacgcgc gaacgccacg aaactcgatg
     1081 ctgtgggcaa caatgcagat cgctgcggga cgttcatgaa aatcgaggag ggacgacggc
     1141 gcgtgaccat cgcgccttcg acagccgaga acacagccgg ctccctcgcc tcggtcaatg
     1201 caagggattt ccagcttgtg cgccgatcca ccgccggcca acacctctgc actccatgaa
     1261 cacgttcgag ctgtcggtgc tctttttcct gcagctcgct ctcgtgctcg tcgtctgccg
     1321 cagcgtgtgc tgggtcgccg ttcggctcgg tcaacctccc gtcgtcggcg agatgatcgc
     1381 gggcgttctc ctcggccctt cgctcttcgg ctacttttgg ccgcacgctc agcaagcgat
     1441 gtttcccgaa gcgtcgcgac cgatcctttt ttccggcgcg cagatcgcgc tcgcgctcta
     1501 catgttcacc gtcggcctcg atttccgaat ggacctcgtg cacacgcggc tgcgcagcgc
     1561 catcg
//
LOCUS       DASPLT010000505         1321 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_107673, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000505 DASPLT010000000
VERSION     DASPLT010000505.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1321)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1321)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1321
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_107673"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1244)
                     /locus_tag="VEA63_05195"
     CDS             complement(<1..1244)
                     /locus_tag="VEA63_05195"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HYD83420.1"
                     /translation="MKQAILVLLTACLCQAGALRVLVRDPSGAAIANATVRAGADAFL
                     TDAAGEARFPSLPAGRVTVAIEAEGFAPYTTRVQVREPLTTFERKLSLAPRSDKVEVG
                     VDPREDATDPRGNFGATALTPQQIEQLPDDPDELERVLQEMAGPGAVMRVNGFRGGRL
                     PHKSQIASIRFRLTPYSAEEHDDGFMHVDIQTKPGMGEWHGDLAVAFGDDSLNARNAF
                     APRREPQQMRRLDVNLMGPVIKNRTSTSFNFSRNDGYDARTSNGFLPDGPFSQLIRLP
                     NEALSGGARLQHALTRSQILRGEYQFERSDRASAANFDLPERVSTTFSNLHQLRLSAL
                     GSISEKTVNELRFQGQWTDNGTSSLTSSPAVIVQGAFHAGGANADSNRVTRAFELTDH
                     VSFNKGKHSFRAGVQIDHLRHRG"
BASE COUNT          237 a          445 c          389 g          250 t
ORIGIN      
        1 ccgcgatgcc tcagatgatc gatctgcact cccgcccgga acgagtgttt acctttgttg
       61 aacgacacgt ggtctgtcag ctcgaacgcg cgagtaacgc ggttcgaatc ggcattcgcg
      121 ccgccggcgt gaaacgcgcc ctgcacgatc acagcggggc tcgacgtcag tgacgacgtg
      181 ccgttgtccg tccactgccc ctgaaaccgc agttcgttga ccgttttttc agagatcgag
      241 ccgagcgcgg acagccggag ctgatgcaga ttgctgaacg tcgtcgagac tctctccggc
      301 agatcgaaat tcgccgccga cgcgcggtcc gaccgctcga actgatactc cccgcgaagg
      361 atctgcgaac gcgtcagcgc gtgctgcagg cgcgccccgc cactcagcgc ctcgttcgga
      421 agacgaatca gttgcgagaa tggcccgtct ggcaggaacc cgttcgatgt cctcgcgtca
      481 tagccgtcat tgcggctgaa attaaagctc gtactggtcc ggttcttgat gaccggtccc
      541 atcagattca catccaagcg tcgcatctgc tgcggctcac gacgcggcgc gaacgcattc
      601 cgggcattga gggaatcgtc gccgaatgcc acagccaggt cgccatgcca ctcgcccata
      661 cccggcttcg tctgtatatc gacatgcatg aagccgtcgt cgtgctcctc cgccgagtac
      721 ggcgtcagcc gaaaccggat ggacgcgatc tgcgacttgt gcggcagccg cccgccgcga
      781 aacccgttca cccgcatgac ggcccccggc cccgccatct cctgcagcac ccgttccagc
      841 tcatccggat cgtcgggaag ctgctcgatc tgctgcggag tgagtgcggt ggcgccgaag
      901 ttcccccgcg gatctgtcgc atcctcgcga ggatccaccc cgacctcgac tttgtcggat
      961 cgtggcgcaa gcgagagctt ccgctcgaag gtcgtcagcg gttcacgcac ctgaacacga
     1021 gtggtgtacg gcgcgaaccc ctccgcctcg atcgcgaccg tgacgcgtcc ggccggcaga
     1081 gaaggaaagc gcgcctcgcc tgctgcgtcg gtgagaaatg cgtccgcgcc ggcccgcacg
     1141 gtggcatttg cgatcgccgc gccggaagga tcgcgcacaa gcacacgaag agcgcccgcc
     1201 tgacaaaggc aggcggtgag taacaccaga atggcttgtt tcatttccgg aacttctcag
     1261 gtttcaggtt cgggttgacc ttcgccgact tcacttcgta ctcttccgtc agtttcccgt
     1321 c
//
LOCUS       DASPLT010000506         2216 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_6550, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000506 DASPLT010000000
VERSION     DASPLT010000506.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 2216)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2216)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2216
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6550"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..854)
                     /locus_tag="VEA63_05200"
     CDS             complement(<1..854)
                     /locus_tag="VEA63_05200"
                     /inference="COORDINATES: protein motif:HMM:NF013097.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HYD83421.1"
                     /translation="MSDIDALPDDDTGVKKSFWDHLKDLRTALLRSTIAIGLAVILCL
                     LIADKLVMILEYPLQRIDIFQSPKPTVTFKIGDTQLGPYQVTPEQFSGLAEGETPQIV
                     YEIGVSEIAGQQVATLKPLPPVQGEKGASLRVRLLNLSPTEAFFVAFRVAIYGGVIVA
                     APFWIYFMGQFFLPALNMRERSVLYQWVVWGFLLFLTGVAMTYFALLPLALHASVEYS
                     HLMGFEAYDWRAESYIGFVTKFIVGMGVGFQFPIVVLLMVKMGLLTHEQLGHYRRHVV
                     VLSLVLGAL"
     gene            complement(999..1934)
                     /locus_tag="VEA63_05205"
     CDS             complement(999..1934)
                     /locus_tag="VEA63_05205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012377043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HYD83422.1"
                     /translation="MKLYVAGHQGMVGSALVRRLSREPDVTIVARTRRELELTDQAAV
                     DRFYATEKPDVAIIAAAKVGGIHANNTYPAEFIYDNMMVAANAVHAAHRHGVKRLLFL
                     GSSCIYPKHAPQPMPEECLLSSALEPTNEAYAIAKIAGLKLCEYYRKQYGVLFHSAMP
                     TNLYGPGDNYHLQNSHVLPALIRKFHEAKEAGRPEVTAWGTGSPRREFLHVDELADAC
                     AFLLRLENPPDWINVGTGTDVTIKELTETVAEVTGFTGRIVWDTSKPDGTPRKLMDVT
                     RLARLGWQAKLSLRDGVAQTYASFLAEKAAGTLRG"
     gene            complement(2012..>2216)
                     /locus_tag="VEA63_05210"
     CDS             complement(2012..>2216)
                     /locus_tag="VEA63_05210"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012377044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HYD83423.1"
                     /translation="DWEKYVAYDKRYERPAEVELLIGDPAKARRQLGWEPKVRFKELV
                     KIMVESDLQLAKREHQISLLPTL"
BASE COUNT          437 a          719 c          641 g          419 t
ORIGIN      
        1 agggcgccga gcacgagcga cagcacgacg acgtgccggc gatagtggcc gagctgttca
       61 tgcgtgagca ggcccatttt caccatcaac agcaccacaa tcggaaattg aaaaccgacg
      121 cccatgccca cgatgaattt cgtcacgaag ccgatatacg actccgcgcg ccagtcgtag
      181 gcctcgaagc ccatcaagtg cgaatactcc accgacgcat gcagcgcgag cggcagcagc
      241 gcgaaatacg tcatcgccac gcccgtcaaa aacagcagga agccccacac cacccactga
      301 taaagcacgc tgcgttcgcg catgttgagc gcaggcagga aaaactgccc catgaaataa
      361 atccagaacg gcgcggccac gatcacgccg ccgtagatcg ccacgcgaaa cgcgacgaag
      421 aacgcctcgg tcggactcaa attgagcaat cgcacgcgca ggctcgcgcc cttttcgccc
      481 tgaaccggtg gcaacggttt caatgtcgcc acctgttggc cggcgatctc cgacacaccg
      541 atttcgtaga cgatttgcgg cgtctcccct tcagccaggc ccgagaattg ctcgggcgtg
      601 acctgatacg gcccgagctg cgtgtcgccg atcttgaacg tcaccgtcgg tttcgggctc
      661 tggaaaatgt cgatccgttg caacggatac tcgagaatca tcaccaattt gtcggcgatg
      721 agcaggcaca gaatcaccgc cagcccgatc gcgatcgtcg aacggagcaa cgcggtgcgc
      781 aggtccttga gatgatccca aaaagatttt ttcacgccgg tatcgtcgtc cggcaacgca
      841 tcgatgtcgc tcatgagcag gccacccgtg ttgaaaggga ggtgccggct cgcacgcgat
      901 taaaaaatgc cggcgtcatc cgcgctgcgt catcgcgcgt gagcagacgc ctgcgttcca
      961 gcccgccgca cgcccacgcc gaactcaccg cccgcgcgtc agccgcgcaa cgtgcccgcg
     1021 gctttttccg ccaggaacga cgcgtaagtt tgggcgacgc catcgcgcag actgagtttc
     1081 gcctgccagc ccagtcgggc gaggcgggta acatccatga gtttgcgcgg tgtgccatcg
     1141 ggtttcgacg tatcccaaac aattctgccg gtgaatccag tcacttccgc aaccgtttcg
     1201 gtgagttcct tgattgtcac gtcggtgccg gtgccgacgt tgatccagtc cggcgggttc
     1261 tcgagccgca gcaggaaagc gcacgcgtcc gcgagttcgt cgacgtgcag gaattcccgt
     1321 cgcggcgaac ccgtgcccca cgctgtcacc tcgggccgac ccgcttcctt tgcttcgtga
     1381 aatttgcgaa tcagcgccgg cagcacgtgc gagttctgca gatggtagtt gtcgccgggg
     1441 ccgtagagat tcgtcggcat cgccgagtga aacagcacgc cgtattgctt tcggtaatac
     1501 tcgcagagtt tcagccccgc gatcttcgcg atcgcgtaag cctcattggt cggctcgagc
     1561 gcgctcgaca gcaggcactc ctcgggcatc ggctgcggcg cgtgcttcgg gtaaatgcac
     1621 gagctgccga gaaacagcag tcgcttcacg ccatggcgat gcgccgcgtg caccgcgttc
     1681 gccgccacca tcatgttgtc gtaaatgaac tcggccggat acgtgttgtt cgcgtggata
     1741 ccgccgacct tcgccgccgc gatgatcgcg acgtccggtt tttccgtggc gtaaaagcga
     1801 tccaccgccg cctggtcggt gagttcgagt tcgcggcgcg tgcgcgcgac gatcgtcacg
     1861 tccggctcgc gcgacaaccg ccgcacgagc gcagagccca ccattccttg atgtccggca
     1921 acgtagagtt tcatttttca gcgacaggtt cgcagtcgat tcaaaggcga atagagacac
     1981 ggcgccctcg ccgcgccgcc ctcgtccgat tttagagcgt cggcaacaag ctgatctggt
     2041 gctcgcgttt cgcgagctga agatcggatt cgaccatgat cttcaccagt tccttgaaac
     2101 gcaccttcgg ttcccagccg agctgacgcc gggccttcgc gggatcgccg atcaggagtt
     2161 cgacttccgc cggccgctcg tagcgcttgt cgtaggcgac atatttttcc cagtcg
//
LOCUS       DASPLT010000507         1228 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_293398, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000507 DASPLT010000000
VERSION     DASPLT010000507.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1228)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1228)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1228
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_293398"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..604)
                     /locus_tag="VEA63_05215"
     CDS             complement(<1..604)
                     /locus_tag="VEA63_05215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD83424.1"
                     /translation="MAAEETKSASPRRVLIEGLNERIEDLRWTDFEGRDVSSRLVPQS
                     KGENITVSLARPDQFIVKPKSISTVSALEAGKKEVMPGGIVVPLVAAPDGSTEPSTAW
                     FRLTLEVSPTPATWDDAARSYLTRLTFGLRRPPQLPAEVQLEQPVLIKLGFEGMTAGE
                     VAPVRLEAAGLENEQTVDLHFNPRTAAPKVLVRSTLSDVNV"
     gene            838..>1228
                     /locus_tag="VEA63_05220"
     CDS             838..>1228
                     /locus_tag="VEA63_05220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD83425.1"
                     /translation="MNPSAPSANSSFWYALRRTMPPWSFEDNLRELVGVLPRYRVDEV
                     IVKVDTEEFSHGHVPLEWLQRYQPRLHQARDALAEIGVTYSLNPWMTLGHIDRGRDGR
                     KTIPDLQPIVGHDGAVCACCACPISDAW"
BASE COUNT          227 a          414 c          355 g          232 t
ORIGIN      
        1 ccacgttcac atccgacagg gttgaccgca ccagcacttt cggcgcggcc gtgcgtggat
       61 tgaaatgcag atcgacggtc tgctcgttct ccagccccgc cgcttcgagc cgcaccggcg
      121 cgacttcgcc ggccgtcatg ccctcgaaac ccagtttgat caacaccggt tgctccagtt
      181 gcacctccgc gggaagctgc ggcggccggc gaagcccgaa ggtcagccgc gtgagatagc
      241 tcctcgccgc atcgtcccac gtcgccggcg ttgggctcac ctcgagcgtg aggcgaaacc
      301 atgccgtgct tggctccgtc gaaccatccg gcgccgccac aagcggcacg acgattccgc
      361 ccggcatcac ttcctttttc cccgcttcaa gtgccgacac ggtggagatc gatttcggtt
      421 tcacgatgaa ctgatccggt cgcgcgagag aaaccgtgat gttctctccc ttcgactgcg
      481 gcaccagccg cgaggaaaca tcgcggcctt cgaaatcggt ccagcgcagg tcctcgatgc
      541 gttcattcaa tccttcgatc aacaccctcc gcggcgaggc ggatttcgtt tcctcggccg
      601 ccaacgcggc acagcaaccg attgagaaaa gccagcagtg aaacaggggg aagcgcatgg
      661 agaagcgggg aagcctcagc gcgcgctggc agcggccgat cgatggaggc tgagagacgt
      721 tcgagtccgg cgcaagcacg ccggcgaaca cagcgaggta tttcgccaac cttgaaggtt
      781 ctcctttttt ccgatccttg gttgcccgtg actactgcgc atcgatctgt gggcccgatg
      841 aacccgtcgg caccatcagc aaactcttcc ttctggtatg cgctgcgccg cacgatgccg
      901 ccatggtctt tcgaggacaa tctgcgcgag cttgtcggcg ttcttccgcg ttaccgcgtc
      961 gatgaagtga tcgtcaaggt cgacaccgag gaattctccc acggccacgt gccgcttgaa
     1021 tggctgcaac gctaccaacc gcgcctgcat caggcgcgcg acgcactcgc tgaaatcggc
     1081 gtcacctact cgctcaatcc atggatgacg cttgggcaca ttgatcgtgg ccgcgacgga
     1141 cgaaaaacga ttcccgacct acagcccatc gtcggccacg acggcgccgt ctgcgcctgc
     1201 tgcgcgtgtc cgatttccga cgcgtggc
//
LOCUS       DASPLT010000508         2788 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_88443, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000508 DASPLT010000000
VERSION     DASPLT010000508.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 2788)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2788)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2788
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_88443"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..477)
                     /locus_tag="VEA63_05225"
     CDS             complement(<1..477)
                     /locus_tag="VEA63_05225"
                     /inference="COORDINATES: protein motif:HMM:NF012584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HYD83426.1"
                     /translation="MGQYTFKLPDVGEGTAEAEIVAWHVRVGDVVQEDAPLVDVMTDK
                     ATVEMTAPVSGKIVSIHGEPGDMAPVGGAIAVFETEAESSSPASGGGGERSEPEGGVQ
                     STTSTAAKTPAAEGLTATPPKAEPPQAPPTNNGAKPKIKAPAGATWSEDQVQAAMQG"
     gene            complement(481..1494)
                     /locus_tag="VEA63_05230"
     CDS             complement(481..1494)
                     /locus_tag="VEA63_05230"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF014798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HYD83427.1"
                     /translation="MATMTMIQALNSALDTMMERDSKVLTFGEDVGYFGGVFKVTEHL
                     QQKYGPKRCFDAPINECGIAAAAIGMAVYGLRPVIEIQFADYMYPAYDQLVSEAARIR
                     YRSANDYTVPMTVRMPYGGGIYGGQTHSQSPEALFTHVAGLKTVIPATPYDAKGLLIS
                     AIEDDDPVIFLEPKRVYNGPFDGRRDRPVQPWSKHPASEVPDGYYKIPLGKANIVREG
                     ADVTILSYGTMVHVALAAAEDSGIDAEVIDLRTLLPVDIETIVASVSKTGRCVIVHEA
                     TRTSGYGAELSALVQEHCFYHLEAPIQRVTGYDTPYPHALEWEYFPTPPRIIKAMRRV
                     MEG"
     gene            complement(1497..>2788)
                     /locus_tag="VEA63_05235"
     CDS             complement(1497..>2788)
                     /locus_tag="VEA63_05235"
                     /inference="COORDINATES: protein motif:HMM:NF012880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HYD83428.1"
                     /translation="PHRPRTHRRREKELRTAMSSRKSNALDLHIPAPRARPGDKPDFS
                     GWSFPEPGDTPRPDVDTPAVELRDYAYGLVRVLDMEGNALGPWTPHIEPETLRRGLKY
                     MLLTRAFDDRMYRAQRQGKTSFYMQSFGEEAISVAQALALNPDDMCFPSYRQQGILIA
                     RDYPLVEMMHQIYSNARDPMKGRQLPVMYSSKKDGFFTISGNLATQVPQAVGWAMASA
                     YSGDLRIASTWLGEGSTAEGDFHAAMTFANVYKAPVIINVVNNQWAISSFQGIAGGEN
                     TTFAARGVGYGMPPLRVDGNDFLAVYAVTQWAAERARSNMGPTLIEHFSYRAGPHSTS
                     DDPSRYRPAEEGRAWPLGDPIERLKTHLISIGEWTEDQHRQAQEEAVETVRTTGREAE
                     KVGVLGADGAHARQSMFEDVYKDVPWHLRQQSEEEAI"
BASE COUNT          412 a          800 c         1005 g          571 t
ORIGIN      
        1 gccttgcatg gcggcttgca cttggtcttc cgaccaggtg gcgccggcgg gggctttgat
       61 cttgggcttg gcgccgttgt tggtgggcgg cgcttgtggg ggttcggcct ttggcggcgt
      121 cgcagtcagg ccttcggcgg cgggcgtttt tgcggcggtg ctggtggtgg attggacgcc
      181 cccctccggc tcgctgcgct cgccacctcc cccgctcgcg ggggaggatg actccgcttc
      241 ggtttcaaac acggcgatgg cgccgccgac gggggccatg tcgccgggtt cgccgtggat
      301 ggagacgatt ttgccgctga cgggcgcggt catttcgacc gtcgctttgt ccgtcatcac
      361 gtcgacgagc ggggcgtctt cctgcacgac atcgcccacg cgcacgtgcc aggcgacgat
      421 ctcggcttcg gcggtgcctt cgcccacgtc gggcaatttg aaagtgtact ggcccacgcg
      481 tcagccctcc atcacacggc gcatcgcttt gatgatgcgc ggcggggttg ggaaatactc
      541 ccattcgagc gcgtgcggat agggcgtatc gtagcccgtg acgcgctgaa tgggggcttc
      601 gagatgatag aagcaatgct cctgcaccag cgcgctgagt tcggcgccgt agccggacgt
      661 gcgcgtcgct tcgtgaacga tgacgcagcg accggttttg ctgacgctgg cgacgatggt
      721 ctcgatatcg accggcagga gcgtgcgcag atcgatgact tcagcgtcga tgccggaatc
      781 ttcggcggcg gcgagcgcga cgtggaccat ggtgccgtag gagaggatgg tgacgtcggc
      841 gccttcgcgg acgatgttcg ctttgccgag cgggattttg tagtagccgt ccggcacttc
      901 gctggctggg tgcttcgacc agggctgcac ggggcggtcg cggcggccgt cgaacgggcc
      961 gttatagacg cgcttcggtt cgaggaagat gaccggatca tcgtcttcga tggcgctgat
     1021 gaggaggccc ttggcgtcgt agggcgtggc cgggatcacg gttttgaggc cggcgacgtg
     1081 cgtgaagaga gcttccggcg attggctgtg ggtttggccg ccatagatgc cgccgccata
     1141 gggcatgcgt actgtcatcg gcacggtgta gtcgttggcg gagcgatagc ggatgcgcgc
     1201 ggcttccgag acgagttgat cgtaggccgg gtacatgtag tcggcgaact gaatctcgat
     1261 cacggggcgc aggccataga cggccatgcc gatcgcggca gcggcgatgc cgcattcgtt
     1321 gatcggcgca tcgaagcagc gtttcggccc atacttctgc tgcaggtgtt cggtgacttt
     1381 gaacacgccg ccgaaatagc cgacgtcctc gccgaacgtg agcaccttgg agtcgcgctc
     1441 catcatggtg tcgagcgcgg agttcagcgc ctggatcatg gtcatggtgg ccatggtcag
     1501 atcgcctcct cttcgctttg ctgacggagg tgccaaggca cgtccttgta aacgtcttcg
     1561 aacatgcttt ggcgcgcatg ggcgccatct gcgccgagga cgccgacttt ttccgcttcg
     1621 cggccggtgg tgcggacggt ttcgacggct tcttcttgcg cctggcggtg ctgatcttcg
     1681 gtccactcgc cgatggagat gaggtgcgtc ttcaagcgct cgatcggatc gccgagcggc
     1741 caggcgcggc cttcttcggc tgggcgatag cgcgaaggat cgtcggaggt ggagtgcggg
     1801 ccggcgcgat agctgaagtg ttcgatcaaa gtcgggccca tattcgagcg cgcgcgttcg
     1861 gcggcccatt gggtgacggc gtagacggcg aggaaatcgt tgccgtcgac tcgcagcggc
     1921 ggcatgccgt agccgacgcc gcgggcggcg aaggtggtgt tctcgccgcc ggcgatgccc
     1981 tggaacgagc tgatcgccca ttgattgttg acgacgttga tgatgaccgg cgccttgtag
     2041 acgttggcga aggtcatggc ggcgtggaag tcgccctcgg cggtcgagcc ttcgccgagc
     2101 cacgttgaag cgatgcgcag atcgcccgaa tacgccgacg ccatcgccca gccgacggct
     2161 tgcggcactt gcgtagccag attgccggag atggtgaaga agccgtcctt cttggacgaa
     2221 tacatcaccg gcagttggcg ccccttcatg gggtcgcgcg cgttcgagta gatttggtgc
     2281 atcatctcga cgagcggata atcgcgcgcg atcaggatgc cttgctgacg atagctcggg
     2341 aagcacatgt cgtccgggtt gagcgcgagc gcttgcgcga ccgagatcgc ttcttcgccg
     2401 aagctctgca tgtagaagct ggtcttgccc tgacgctggg cgcggtacat gcggtcgtcg
     2461 aaggcgcgcg tgagcagcat gtattttagg ccgcggcgca acgtttccgg ctcgatgtgc
     2521 ggcgtccagg ggccgagtgc gttgccttcc atgtcgagca cacggacgag gccgtaggcg
     2581 tagtcgcgca gttcgacggc gggggtgtca acgtccgggc gcggcgtgtc gccgggctcg
     2641 gggaaggacc agccgctgaa atcgggctta tcgcccggcc gtgcgcgcgg cgcagggata
     2701 tgcagatcga gagcgttcga tttgcgcgag ctcattgctg tcctcagttc tttttcgcgg
     2761 cggcgatgcg ttcgcggacg atgcggtc
//
LOCUS       DASPLT010000509         1736 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_230892, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000509 DASPLT010000000
VERSION     DASPLT010000509.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1736)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1736)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1736
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_230892"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            193..714
                     /gene="mreD"
                     /locus_tag="VEA63_05240"
     CDS             193..714
                     /gene="mreD"
                     /locus_tag="VEA63_05240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HYD83429.1"
                     /translation="MRRTLAIFATLFLLWAIVAQVNHALSGVHVYLWVGGLFITYSAL
                     TLPLRSGLAASLLGGLLVDATTSVSFGLHALLFALAHAFIFNIRDRLPRDETTARVIV
                     ALFANLALFLVFSFLQIGRVPTPSTVWPRLILDLLCSQVFLTLVAPWFFALQARTLEA
                     ADAFAALYERRFQ"
     gene            724..>1736
                     /locus_tag="VEA63_05245"
     CDS             724..>1736
                     /locus_tag="VEA63_05245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012377387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase"
                     /protein_id="HYD83430.1"
                     /translation="MSSAETNLAKRSGRLVETHRSYDPRIVLFYFILAALLLTLVAGL
                     AYQQLIKTDTYHERERVQNQRRVLVPGPRGNIYDREGRLLVRNRPRFAVVLYLDELRA
                     EFRRSYLTVRNNYRQSGDKDLPSSSQMEQIARATVVQRYLDQVNAILGRSEKVDTADL
                     RKHFVRELLLPYTLIGDLAPEEYARLIERLPVQSPLQVYTSSTRDYPYGSAAAHTLGY
                     VSVDPDIDNQNLPGEDLTTFKMKGSVGKTGLEAAFDSHLQGEAGGTIFRVDPAGYRVN
                     PPLEKRLPVQGKSLTTSLDIDLQLVAEQAIGEFAGAAVALDVRTGEVLVLASKPDYNL
                     ADTS"
BASE COUNT          287 a          676 c          419 g          354 t
ORIGIN      
        1 gttcaaaagt ggcgacgttc aactcgaccc gaaactctcc agcgtcaccg aggtcaccgt
       61 gctggtgccg ctcaatcccg agtgatcgat ttttcagcta agagctgaga gccaagagcc
      121 cgaactccca atccttcgcc actccccgcg ctcagctcta gactctcagc tctcagcttc
      181 gttttcccag ccatgcgccg caccctcgcc atcttcgcca ccctgtttct cctctgggcg
      241 attgttgcgc aggtaaatca cgcgctctcc ggcgtgcatg tttatctttg ggtcggcggg
      301 cttttcatca cttactccgc cctcacgctt ccgctccgct ccggactcgc cgcgtcgctg
      361 ctcggcggct tgctcgttga cgccactacc tccgtttcgt tcggcctcca cgcgctgctg
      421 tttgcgctcg ctcacgcctt catcttcaat atccgcgacc ggctgccgcg cgacgaaacc
      481 accgcgcgtg tcatcgtcgc tctcttcgcc aatctcgcgc ttttcctcgt gttttctttt
      541 ctccaaatcg gacgcgtgcc gacgccctcc actgtctggc cgcggctcat cctcgacctc
      601 ctctgctcgc aggtttttct cacgctcgtc gcgccctggt tcttcgccct ccaagcgcgc
      661 acgctcgaag ccgccgacgc gtttgccgcc ctctacgaac gccgcttcca gtaacgccgg
      721 ccgatgtctt ccgccgaaac caatctcgcc aaacgctccg gccgcctcgt cgaaacgcac
      781 cgcagctacg acccgcggat cgtcctcttc tatttcattc tcgccgcgct cctgctcacg
      841 ctcgtcgccg ggctcgctta ccagcagctg atcaagaccg acacctatca cgagcgcgaa
      901 cgcgtccaga atcagcgccg cgtcctcgtt cccggcccgc gtggcaacat ctacgatcgc
      961 gagggccgtc tcctcgtccg taaccgcccg cgcttcgcgg tcgtgcttta cctcgacgag
     1021 ctccgtgccg agtttcgccg ctcctacctc acggtccgta acaactaccg ccagtccggc
     1081 gacaaagacc tgccctcgtc gtcgcaaatg gagcagatcg cccgcgcgac cgtcgtgcag
     1141 cgctacctcg atcaggtcaa cgcgatcctc ggccgcagtg aaaaagtgga tacggccgat
     1201 cttcgcaaac acttcgttcg cgagctgctc ctgccctaca cgctcatcgg cgatctcgcc
     1261 cccgaggaat acgcgcggct catcgaacgc ctccccgttc aatcgcccct ccaagtctac
     1321 acctccagca cgcgcgatta tccctacggt tccgccgcgg cccacacgct cggctacgtc
     1381 agcgtcgatc ccgacatcga taatcaaaat ctccccggcg aagacctcac gaccttcaag
     1441 atgaagggca gcgtcggcaa aaccggcctc gaagccgcgt tcgattcgca cctccaaggc
     1501 gaggccggcg gcaccatctt ccgcgtcgat cccgccggtt accgcgtgaa tcccccgctc
     1561 gagaaacgcc tgcccgtgca gggcaagagc ctcaccacgt cgctcgacat cgatctccaa
     1621 ctcgtcgccg aacaagccat cggcgagttc gccggcgccg cggtcgcgct cgatgtgcgc
     1681 accggcgagg tgctcgttct cgcaagcaaa cccgattaca atctcgccga cacctc
//
LOCUS       DASPLT010000510         1998 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_66592, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000510 DASPLT010000000
VERSION     DASPLT010000510.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1998)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1998)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1998
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_66592"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1598
                     /locus_tag="VEA63_05250"
     CDS             <1..1598
                     /locus_tag="VEA63_05250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD83431.1"
                     /translation="LTAGARGSDGPQPFAEDTVAYFSGAVAVKHSRSTSTLWQLDVLH
                     YEGNPSPGVVEYRATPSSRIVGPYRPLVDFHAFGPNTGVRRDVASLSGQLESQLSSSL
                     SLRAAAQWFMREIEQDRFTTGQYVLSTGRFSGVREPRHIEQSFQGAMAQTDLTWRFFG
                     LGGEHKALLGLESTWVNGGNTQRALAVSDRNLLPASVRLFDPDAPDYSRPAYDPALYQ
                     RLITDRRDEILFSALVFNHRTALQGGRTVFTSGLRQDFADIDVRDRRPDAPHAQAEDM
                     TGRLTFHAGANRTIAKGRALVFANVSSAFEPSTRVDGRTGEIQGNERTFGYESGLRTV
                     LFDRQLSVSLTGFQYFNDNIARRNPRYDDPIADADQKQPQLISSGAERFTGAVVQAGW
                     KASPSWTFAGRAAYTRAVTTASPDVPEEVGRALTRLPALSLTGSVRHAWNAGPLRGFS
                     WGINAVHVGEVVAHYANAVRERLDYPSYTLVGANIGYLLKRKSHAWNLRLNLSNAFDI
                     DLVDLLARPGSGPELSASCRVTF"
     gene            complement(1805..>1998)
                     /locus_tag="VEA63_05255"
     CDS             complement(1805..>1998)
                     /locus_tag="VEA63_05255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HYD83432.1"
                     /translation="QWFRNDGNSNFTPIVLAHAPTHLLSIVALQVEGSPKPALMSGAF
                     ATYPPYDRASRFLLWRQKN"
BASE COUNT          347 a          591 c          690 g          370 t
ORIGIN      
        1 cgctgaccgc gggcgcgcga ggcagcgacg gtccgcagcc gttcgcggag gacacggtgg
       61 cgtatttttc cggcgcggtg gcggtgaagc attcgcgcag cacgtcgacg ctgtggcagc
      121 tcgacgtgct gcattacgaa gggaacccga gtcccggcgt ggtggagtat cgcgcgacgc
      181 cgagctcgcg catcgtcgga ccttaccgtc cgctcgtcga ctttcacgcg ttcggcccga
      241 acaccggcgt gcgtcgcgac gtggcgagct tgagcgggca gctcgagagc cagttgagtt
      301 ccagcctcag cctgcgggcg gctgcgcaat ggttcatgcg cgagatcgag caggatcgtt
      361 tcacgacggg acaatacgtg ctttcgacgg ggcggttttc gggtgttcgc gagccgcggc
      421 acatcgagca gagctttcag ggcgcgatgg cgcagaccga cctgacgtgg cggtttttcg
      481 gattgggcgg cgagcacaag gcgctgctgg gcctcgagag cacgtgggtg aacggcggca
      541 atacgcagcg cgcgctcgcg gtgagcgatc gcaacctgct gccggcttcg gtgcggttgt
      601 tcgatccgga tgcgccggac tacagccggc cggcgtatga tccggcgctt tatcaacggc
      661 tgatcacgga ccggcgcgac gagattcttt tcagcgcact ggtgttcaac catcggaccg
      721 cgctccaagg cgggcgcacc gtgttcacgt cgggtttgcg ccaggacttt gccgatatcg
      781 atgtgcgcga ccggagaccg gatgcgccgc acgcgcaagc ggaagacatg acgggccggc
      841 tgacgtttca cgcgggagcg aaccgcacga tcgcgaaagg tcgcgcgctg gtgttcgcaa
      901 acgtcagctc cgccttcgag ccgtcgacgc gcgtcgacgg acgcaccggc gagattcagg
      961 gcaacgagcg gacattcggc tacgaatcgg gactgcggac cgtgctgttc gaccgacagc
     1021 tcagcgtctc gctgacgggc tttcagtatt tcaacgacaa catcgcgcgg cggaatccgc
     1081 gttacgacga cccgattgcc gacgcggacc agaagcagcc gcagttgatt tcgagcggcg
     1141 ccgagcggtt caccggagcg gtggtgcagg cgggttggaa agcgtcgccg tcgtggacct
     1201 ttgccggacg cgcggcgtat acgcgcgcgg tgacaacggc gtcgccggat gtgccggagg
     1261 aagtggggcg cgctttgacg cgactgccgg cgttgagtct gaccggatcg gtgcggcacg
     1321 cgtggaacgc ggggccgcta cgtggattct cctggggaat taacgcggtg cacgttggcg
     1381 aggtggtcgc gcactatgcg aatgccgtcc gagaacgcct cgactatcca agctacacac
     1441 tggtcggggc gaacatcggc tatctattga agcggaagag ccacgcctgg aacctgcggc
     1501 tgaatctcag caatgccttc gatatcgatc tcgtcgacct tctcgcgcgc ccgggttccg
     1561 gtccggagtt gagcgcgagt tgtcgtgtga cgttctaacc gcgacacgga gttcacggag
     1621 gtggatgcca aatccaaacg agaacgcttg gcctctatta gcatctactc gcatgaaacg
     1681 cagccaacac cttgtggcct cctgcttcct ctgtgtcggc gctctgtgca ctctgtgatc
     1741 cacgccttgc tcggtttcgg cggagaattt ttcgccgccg tgtgtgaggc ctcaagaagc
     1801 gcaatcagtt tttctgccgc cacaggagga atcggctggc gcggtcgtag ggcggatacg
     1861 tggcgaacgc gcctgacatc agcgcgggtt tcggcgagcc ctccacttgc agcgccacga
     1921 tcgagagaag atgtgtcggt gcgtgagcga gcacgatggg cgtgaagttg gagtttccgt
     1981 cgttacgaaa ccactgca
//
LOCUS       DASPLT010000511         2466 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_230902, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000511 DASPLT010000000
VERSION     DASPLT010000511.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 2466)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2466)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2466
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_230902"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..977)
                     /locus_tag="VEA63_05260"
     CDS             complement(<1..977)
                     /locus_tag="VEA63_05260"
                     /inference="COORDINATES: protein motif:HMM:NF015803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ShlB/FhaC/HecB family hemolysin
                     secretion/activation protein"
                     /protein_id="HYD83433.1"
                     /translation="MRSLLLLLAAMIGATVAFAQTSPAEQTAFELFAARPNEERLADK
                     LAGLRIVATPAQLTEPGFPADGVDTSRVAWMPPEDIIFAVAGFMDMPVSRESLDRLEM
                     AVQLFARTRGKTFVSIYLPPQDITGGYVQIVASEATNAGGVTVRGAKYFSEKSYRNAL
                     SQEDGKPIDEARLRADVEWLNRNPFRRAVIAAEPGAEPGTTRLALRVQESAPWQLSAG
                     YDNTGTKTTGEDRVSANVLWGNALGLGHQMSYRFTSDPEMEHSRSHSLSYAADLPWRH
                     SVSVFAAWSDIESIMPEPFTQEGSSGQAGVRYVIPLTNARKGWVQSVTLG"
     gene            complement(979..>2466)
                     /locus_tag="VEA63_05265"
     CDS             complement(979..>2466)
                     /locus_tag="VEA63_05265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD83434.1"
                     /translation="PNLYFDLRSENEAQGVGEAVDLASIAFPARPSRTPPQDTSAPPP
                     TTLPPGTVEFVPGKIVIPSSPTPVTYSEVIVLDTSITLGPPLILPGVPTKDTTKDRFA
                     DVTRYIIPRAYGANNYVVTKVTNGVLTMKADPDIKADTLEREAAAAKRKADRDAFYGT
                     TPVAPGEIRLAGTYAFGLPRDMLPTLRAAIGSYLNDEITKGLADEPGSLAAAINGGLQ
                     VTSWDQLPEDKIMAFLADIHTNPAKQAQVMPALMGYTMGVAQLDPAKWSDQDKQLMAF
                     ISPALKDARNTFIDEAKQARDDWVATEGAARGATFKGLLDGKVPYEKIVANAVGETMA
                     EAMQTFQQKAEEFDATKLSAIGAGAAAGAIAGSIAGIQNLVTQTFPYAKGAVELALKR
                     AGEKVTSEAMSKGLGRLMGSTGGGVGTIVSLAVTILITESMAIADNEKAAANFNALLN
                     SGNDPIDPNGMMKSDQGKAVMLLGLSKLFLGGGGQKEISTNFDLN"
BASE COUNT          408 a          834 c          733 g          491 t
ORIGIN      
        1 ccgagggtca cgctctgcac ccagcccttg cgtgcgttcg tcagcggaat cacataacgc
       61 acgcccgcct gtcccgagct gccttcctgc gtgaagggct ccggcatgat cgactcgatg
      121 tccgaccacg ccgcaaacac cgacaccgaa tgccgccacg gcaaatccgc cgcatacgag
      181 agcgagtgac tgcgcgaatg ctccatctcc ggatcgctcg tgaaacggta gctcatctga
      241 tgcccgagcc ccagcgcgtt gccccacagc acattcgccg aaacgcgatc ctcgcccgtc
      301 gtcttcgtgc cagtgttgtc gtagcccgcc gacaactgcc acggcgcgct ctcctgcacg
      361 cgcagggcca gtcgtgttgt gccgggctct gcgccgggtt ccgccgcgat caccgcccgc
      421 cgaaacggat ttcggttcaa ccactccacg tccgcgcgca gtcgcgcttc gtcgatcggt
      481 tttccgtcct cctggctgag cgcgttgcga tagcttttct cggaaaaata tttcgcgccg
      541 cgcaccgtca cgccgcccgc attcgtcgcc tcgctcgcaa cgatctgcac ataaccgccc
      601 gtgatgtcct gcggcggcag atagatcgaa acgaaggtct tcccgcgcgt tcgcgcgaaa
      661 agctgcacgg ccatttcgag ccggtcgaga gattcgcgcg acaccggcat atccatgaaa
      721 cccgcaaccg cgaagatgat gtcttccggc ggcatccacg ccacgcggct ggtatccacg
      781 ccgtcagcgg gaaatccggg ttcggtcaac tgcgcgggcg tcgcgacgat gcggaggccg
      841 gccagtttgt cggcgagccg ttcttcgttc ggtcgtgccg cgaagagttc gaacgccgtc
      901 tgttcggccg gcgaggtttg cgcgaacgca actgtggcgc cgatcatcgc cgcaaggagg
      961 agaagcaggg aacgcatgtc agttcaggtc gaagttggtg gagatttcct tctgcccgcc
     1021 gccaccgagg aacagtttcg agaggccgag cagcatgacc gccttgccct gatcgctctt
     1081 catcatccca ttcgggtcga tcggatcgtt gccggagttc agcagcgcgt tgaaattcgc
     1141 cgccgccttt tcgttgtcag cgatcgccat cgattcggtg atcaggatcg taaccgcgag
     1201 agacacgatg gtgcccacgc cgccacccgt cgaacccatg aggcggccca agcccttgct
     1261 catagcctca ctcgtgacct tttcgccggc gcgcttcagc gcgagttcaa ccgcgccttt
     1321 ggcatagggg aacgtttgcg taaccagatt ctggatgccc gcgatcgatc ctgcgatcgc
     1381 acccgcagcc gcaccggcgc ctatcgcact gagcttcgtc gcgtcgaact cctcggcctt
     1441 ctgctggaag gtttgcatcg cctctgccat cgtctcgccg accgcgtttg ccacgatctt
     1501 ttcgtaaggc actttgccat cgagcagccc cttgaaagtg gcacctcgtg ccgcgccttc
     1561 cgtcgccacc caatcgtcgc gcgcctgctt ggcctcgtca atgaacgtgt ttcgagcgtc
     1621 tttcagcgcg gggctgatga acgccatgag ctgcttgtcc tgatcgctcc acttcgccgg
     1681 gtcgagctgc gccacgccca tcgtataacc catcagcgcc ggcatcacct gggcctgctt
     1741 cgccgggttc gtgtgaatgt ccgccaggaa ggccatgatc ttgtcctccg gcaattgatc
     1801 ccaacttgtc acttgcagcc cgccgttgat tgccgccgcg agcgatccag gttcgtcggc
     1861 cagtcctttc gtgatctcgt cgttcaaata ggagccaatc gccgctcgca gcgtcggcag
     1921 catgtcgcgg ggcaatccga acgcgtaggt gccagcgaga cggatttcgc caggcgccac
     1981 cggtgtggtg ccgtagaacg catcgcgatc ggccttccgc ttcgccgcag ccgcctcgcg
     2041 ctcgagcgta tccgccttga tgtcggggtc ggccttcatc gtgagcacgc cgttcgtcac
     2101 cttcgtgacc acgtagttgt tcgcgccata cgcgcgggga atgatgtagc gcgtcacgtc
     2161 ggcaaaccga tccttcgtgg tgtccttcgt cggaactccc gggagtatca gcggcggccc
     2221 aagcgtgatc gatgtgtcga ggacaatgac ctcgctgtaa gtcaccggtg tcggcgagct
     2281 gggaatgact atcttgcccg ggacaaactc gacggtgccc ggaggcaggg ttgtgggcgg
     2341 cggcgccgac gtgtcctgcg gcggcgtgcg cgatgggcgc gcggggaacg cgatcgacgc
     2401 gagatcgacc gcctcgccca ccccttgcgc ctcgttttcc gagcgcaggt cgaaatacag
     2461 attcgg
//
LOCUS       DASPLT010000512         1342 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_29778, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000512 DASPLT010000000
VERSION     DASPLT010000512.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1342)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1342)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1342
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_29778"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..366
                     /locus_tag="VEA63_05270"
     CDS             <1..366
                     /locus_tag="VEA63_05270"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HYD83435.1"
                     /translation="FATARNAALDIFRRRKIVSIEPVGDLAALNVIEETPDAADTVNK
                     QQELELLAAAVRTLPERCRQVITLRLLYGLSHKEIARDLRISEHTVKAQLAKGMRRCA
                     AYFEERGLLTRVLPENPTP"
     gene            363..>1342
                     /locus_tag="VEA63_05275"
     CDS             363..>1342
                     /locus_tag="VEA63_05275"
                     /inference="COORDINATES: protein motif:HMM:NF016648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HYD83436.1"
                     /translation="MRTFPKKCPSDDEAIEARAAAWLAQRDDGMSPEDEVAFATWCAA
                     DRRHAVAVARLESAWTALAQLRDYRPTARAHPDRDLLAPHSGARLLVFPKWVAAAAVA
                     AAAIAVTLTWHWRSLPLAPALGTAQVYTTTVGGYQRLTLPDGSIVELNDSSEVRVRYT
                     PDTRFVQLVGGQAHFTVAKNKTRPFIVETRGVAVRAVGTAFDVRLAQDQVEVLVTEGR
                     VQLDRLQPPASRTAPAAAKPSLLDAGWRAVVPHERQLAVALEYLPPERIRETLSWQGP
                     RLVFVETPLAEVVEQFNRRNRIRLSLGDDELAALPVGGSFRAENVESFVR"
BASE COUNT          234 a          515 c          376 g          217 t
ORIGIN      
        1 ttcgccaccg cccgcaacgc cgcgctcgat attttccgcc gccggaaaat tgtttcgatc
       61 gaaccggtag gcgatttggc cgcactgaac gtcatagagg aaacgcccga tgccgccgac
      121 accgtcaaca aacagcagga actcgagctc ctcgccgccg ccgtccgcac gctgcccgaa
      181 cgttgccgcc aggtgatcac actgcgcttg ctctacggac tttcgcacaa ggaaatcgcc
      241 cgcgacctcc gcatctccga acacaccgtc aaagcccagc tcgcgaaagg catgcgccgc
      301 tgcgccgctt atttcgagga acgcggcctc ctcacccgcg ttctgccgga aaacccaacg
      361 ccgtgagaac ttttccaaaa aaatgtccgt ccgacgacga agcgatcgaa gcacgcgccg
      421 ccgcgtggct cgcgcaacgc gacgacggca tgagccccga agacgaagtc gcgttcgcca
      481 cgtggtgcgc ggccgatcgg cgtcacgcgg tggcggtggc gcgactcgag tccgcgtgga
      541 ccgcactcgc gcagctccgc gattaccgcc ccaccgcgcg cgctcatccc gatcgcgatc
      601 tgctcgcgcc gcactccggc gcccgtctcc tcgtgtttcc caaatgggtc gccgccgccg
      661 cggtcgccgc agccgcgatt gcggtcacgc tcacctggca ttggcgttcg ctgcccctgg
      721 cgcccgcact cggcaccgcg caagtctaca cgaccaccgt cggcggttat caacgtctca
      781 cgctgccgga cggttccatt gtcgaactca acgacagcag cgaagtgcgc gtccgttaca
      841 ctcccgatac gcgcttcgtt caactcgtcg gcggacaagc ccacttcacc gtcgcgaaaa
      901 acaaaacgcg gccgttcatc gtcgaaaccc gcggcgtcgc cgtccgcgcg gtcggcaccg
      961 ctttcgacgt ccgcctcgcg caagaccagg tggaggtgct cgtcaccgaa ggccgcgttc
     1021 aactcgaccg cttgcaaccg cccgcgtcgc gcactgcacc cgccgccgcg aagccgtcgc
     1081 tgctcgatgc cggctggcgc gccgtcgtcc cgcacgaacg ccagcttgcc gtcgcgctcg
     1141 agtatcttcc acccgaacgc attcgcgaaa cgctctcgtg gcagggcccg cgtctggtgt
     1201 ttgtcgaaac cccgctcgcc gaagtcgtcg aacaattcaa ccgccgcaac cgcatccgcc
     1261 tctcgctcgg cgacgacgaa ctcgccgcgc tgccggtcgg cggaagcttc cgcgccgaaa
     1321 acgtcgagtc cttcgtccgt tt
//
LOCUS       DASPLT010000513         1203 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_6613, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000513 DASPLT010000000
VERSION     DASPLT010000513.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1203)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1203)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1203
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6613"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1203
                     /locus_tag="VEA63_05280"
     CDS             <1..>1203
                     /locus_tag="VEA63_05280"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007362690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HYD83437.1"
                     /translation="GFTDPLVDCKVSKQRYRADQLFFSPVIVDGKTVGYVSALESENT
                     GGELQSAAESFKRKKAIQGTLAPVQPRDFTEAKPDEIALIPSPATGEPGSLTEPRSFN
                     MMFQTNVGAMTDGSSIAYLRPETAQGMFVDFKNVVDTGRVKLPFGIAQIGKSFRNEIT
                     PRNFIFRSREFEQMEMEYFIHEDADWKKAHDEWIQWCKNWLLSIGLPESHLSLYEHPK
                     EKLSFYSRGTVDIMFKYPFGVQELWGIAARGNYDLTQHANASGKPQDIFDEATKKKFV
                     PHVIEPAVGTDRIFLAVLCAAYAEEDVKDEKGNVEKRTVLRLSPRIAPVKVAVLPLLK
                     NKEQLVGRAKELYAKLKRKYAVFYDEAGAIGRRYRRQDEIGTPWCVTIDFDTIEKDGT
                     FTLRDRDSM"
BASE COUNT          271 a          437 c          302 g          193 t
ORIGIN      
        1 ggcttcaccg acccgctcgt cgattgcaaa gtctcgaaac aacgctaccg cgccgaccag
       61 ctcttcttct cgcccgtcat cgtcgacggc aaaaccgtcg gctacgtttc cgcgctcgag
      121 tccgaaaaca ccggcggcga actccagtcc gccgccgaat ccttcaagcg caagaaggcg
      181 atccaaggca cgctcgctcc cgtccaaccg cgcgacttca ccgaagcgaa acccgacgaa
      241 atcgcgctca tcccctcgcc cgccaccggc gaacccggca gtctcaccga gccgcgttcg
      301 ttcaacatga tgttccagac gaacgtcggt gcgatgaccg acggctcgtc catcgcctac
      361 ctccgccccg aaaccgcgca gggcatgttc gtcgatttca agaacgtcgt cgacaccggc
      421 cgcgtgaaac tccccttcgg catcgcccaa atcggcaagt ccttccgcaa cgaaatcacc
      481 ccgcgcaact tcatcttccg ctcccgtgaa ttcgagcaga tggagatgga atatttcatc
      541 cacgaagacg ccgattggaa aaaagcccac gacgaatgga tccagtggtg caaaaactgg
      601 ctgctctcga tcggcctccc cgagtcgcac ctttcgctct acgagcaccc gaaggagaaa
      661 ctctcgttct actcccgcgg caccgtcgac atcatgttca aatacccgtt cggcgtgcag
      721 gagctctggg gcatcgccgc ccgcggcaac tacgacctca cgcaacacgc caacgcctcc
      781 ggcaaaccgc aggacatctt cgacgaagcc acgaaaaaga aattcgtccc gcacgtcatc
      841 gagcccgccg tcggcaccga ccgcatcttc ctcgccgtcc tctgcgccgc ctacgcggaa
      901 gaggacgtga aagacgaaaa gggcaacgtc gagaaacgca ccgtcctccg cctctcgccc
      961 cgcatcgcgc cggttaaagt cgccgttctc ccgctcctca aaaacaaaga gcagctcgtc
     1021 ggccgggcga aagaactcta cgcgaagttg aagcgcaaat acgccgtctt ctacgacgaa
     1081 gccggcgcga tcggccgccg ctatcgtcgc caggacgaga tcggcacgcc gtggtgcgtg
     1141 acgatcgact tcgacacgat cgagaaagac ggcacgttca ccctccgcga ccgcgattcg
     1201 atg
//
LOCUS       DASPLT010000514         1702 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_6631, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000514 DASPLT010000000
VERSION     DASPLT010000514.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1702)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1702)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1702
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6631"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..939)
                     /locus_tag="VEA63_05285"
     CDS             complement(<1..939)
                     /locus_tag="VEA63_05285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012377414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HYD83438.1"
                     /translation="MKLNLLPVLLFAFTTASLSAAAASSAPTTPPATVAGFTFVKTVG
                     DISEYTLDANGLTVLMMPEHSSPTLTFMVTYRVGSRNEVTGTTGATHLLEHLMFKGTP
                     AFNREKGNSVDQFLERVGGIYNATTGFDRTNYYANIGNEHLEGYMAIEADRMRNLWLR
                     DSDRQPEMTVVRNEFERGENSPFMALYKELFQTAFVAHPYHHLPIGHRSDIERVPIEK
                     LKEFYDTFYWPNNATVSIIGDFQPADALALVQRYYGVFPRSPHPIPELYTEEPDQRGA
                     RRTTVKRAGELGVVALGHKICAATHPDFAPLSIASAI"
     gene            complement(1391..>1702)
                     /locus_tag="VEA63_05290"
     CDS             complement(1391..>1702)
                     /locus_tag="VEA63_05290"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HYD83439.1"
                     /translation="GFTTVQAVHGREAVEFFQREPNGFRAVLLDLTMPVMDGEEAFNE
                     IHRLNPAVPVILMSGYNKTDSAGRFEGRGLAGFVQKPFEASRLMETLRTVLEEQPSAR
                     R"
BASE COUNT          295 a          451 c          618 g          338 t
ORIGIN      
        1 aatcgcgctg gcgatcgaga gcggggcgaa gtcgggatgc gtggcggcgc agatcttgtg
       61 gccgagcgcg acgacgccga gttcgccggc gcgtttgacg gtggtgcgcc gggctccgcg
      121 ctgatcgggt tcttccgtgt agagctcggg gatcgggtgc ggcgaacggg gaaacacgcc
      181 gtagtagcgt tggacgagcg cgagcgcgtc ggctggctgg aaatcgccga tgatcgaaac
      241 ggtcgcattg ttcggccagt agaacgtgtc gtagaactcc ttcagctttt cgatcggcac
      301 gcgctcgatg tcgctccgat ggccgatggg caggtgatga tagggatgcg cgacgaaggc
      361 ggtttggaag agctccttgt agagcgccat gaagggcgag ttttcgccgc gctcgaattc
      421 gttgcgcacg acggtcatct cgggctggcg gtcggagtcg cggagccaga ggttgcgcat
      481 gcggtcggcc tcgatcgcca tgtagccctc gaggtgttcg ttgccgatat tggcgtagta
      541 gttggtgcga tcgaagccgg tcgtggcatt atagataccg ccgacgcgtt cgagaaactg
      601 gtcgacgctg ttgcccttct cgcggttgaa ggcgggcgtg cctttgaaca tcaggtgctc
      661 gagaagatgc gtggctccgg tggtgccggt gacttcgttg cgcgagccga cgcggtaggt
      721 gaccatgaac gtgagcgtcg ggctcgaatg ctcgggcatc atcagaacgg tgaggccgtt
      781 cgcatcgagc gtgtattcgg agatatcgcc aacggttttg acgaaggtga agcccgcgac
      841 cgtcgcgggt ggcgttgttg gcgcggacga cgcggcggcg gcggagagcg aggccgtggt
      901 gaacgcgaac aggagaacgg ggagcagatt caatttcatg cgaaagaagg ctggcggcca
      961 aggtgcgcaa accgtgggaa aggccaagcc gtttgcgttg aacttcggcg aagagcgcac
     1021 aggcggcgga tcggcgtgat gaacggcgtg gcgccgcgtc gcgcgcgcgg cctgatagcg
     1081 gttaaattat accgcggccg tggcggggtt ggggtaggag cccgccttcg gtgctacgat
     1141 cgaggcgcta cagcattttc agccaaacca tagccgctca gatggagggc cggcctccgt
     1201 gccggccgca gacgacacgg aggtcgtccc tccaaaacgg ccgcagcttt ttgaaaacgc
     1261 gcttgtaggg tgtcccggag gtatttttaa taattcggcg ttcttcccag tgcgcggacg
     1321 cctcggagag gcgtccctac cggcgctgcc gcgccactcg atttattcgc gtttcttgtg
     1381 gggcaccttg ctagcggcgg gcgcttggtt gctcttcgag cacggtgcgc agggtttcca
     1441 tgaggcgtga cgcttcgaag ggtttttgga cgaagccggc gaggccgcgg ccttcgaagc
     1501 ggccggcgga gtcggtcttg ttgtagccgc tcatcagaat caccggaacg gcgggattga
     1561 ggcggtggat ttcgttaaag gcttcttcgc cgtccatcac cggcattgtg agatcgagca
     1621 acacggcgcg aaagccgttt ggctcgcgtt gaaaaaattc caccgcttcg cggccgtgca
     1681 ccgcttgaac cgtggtgaag cc
//
LOCUS       DASPLT010000515         1045 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_129344, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000515 DASPLT010000000
VERSION     DASPLT010000515.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1045)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1045)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1045
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_129344"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..546)
                     /locus_tag="VEA63_05295"
     CDS             complement(<1..546)
                     /locus_tag="VEA63_05295"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HYD83440.1"
                     /translation="MSSSPKNETPRPHSSVVVDGNDRAAARSMLRAVGFTDEDFRKPQ
                     IAVASSASNMTPCNVHLGDLSEHAQKGVAAAGGKPVYFNTITVTDGISMGTQGMRYSL
                     VSREVIADSIETAVAAEGFDGLIAIGGCDKNMPGAMIAIARLNRPAVFIYGGTILPGF
                     TPGDCDKQKPLDIVSVFEAVGK"
BASE COUNT          210 a          280 c          364 g          191 t
ORIGIN      
        1 tttgccgacg gcttcgaaaa cggagacgat atcgagcggc ttctgtttgt cgcaatcgcc
       61 aggcgtgaag cccggaagaa tcgtgccgcc gtagatgaac accgcggggc ggttgaggcg
      121 ggcgatggcg atcatcgcgc cgggcatgtt tttatcgcag ccgccgatcg cgatgagacc
      181 gtcgaagccc tcggcggcga cggcggtttc gatggagtcg gcgatgactt cgcgggacac
      241 gaggctgtag cgcatgccct gcgtgcccat gctgatgccg tcggtgacgg tgatggtatt
      301 aaagtagacg ggcttgccgc cggcggcggc gacgcccttt tgcgcgtgct cgctgagatc
      361 gccgaggtgg acgttgcacg gcgtcatgtt gctcgcggac gaagcgacgg cgatttgcgg
      421 tttgcggaaa tcttcgtcgg tgaagccgac ggcgcgcagc atggagcggg cggcggcgcg
      481 gtcattgccg tcgaccacga cagaggaatg agggcgcggg gtttcgttct taggcgagga
      541 actcatgatg gaaagcggga gcagaccaag cgcggcgggt gcgggcaagc gcgattgaaa
      601 aggcctccaa tacaattgga ggccttttca atcgcgaagc tccaagcagg gaaaagctcc
      661 aagcaccaac atccaagctc cagggaaaca ccaagcaacg gcgaagctcc aagctccaac
      721 ctccagctcc aatgaagcat caagctccaa ggaccaagga aacacgaagt gctaacagca
      781 aggtcgaagg tgacgtcgga ctctgcgaac ggcggccggg ggccgatggc ggggcggcgt
      841 ttttagcgcg ttagggacga cgcgctccag ctttcggacg aatgatgttt gaagcttctc
      901 tggagcttga atcttggtgc ttggagcttc cgtcggcgcg ggctcgggcg cgccgacggc
      961 ggttttcgtg gttgcgtggg ccgaggggcg ccgcatcact cgtgcgaata tttccgcatg
     1021 gccttcaatt tgaaaaaggt gctca
//
LOCUS       DASPLT010000516         4015 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_45863, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000516 DASPLT010000000
VERSION     DASPLT010000516.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 4015)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4015)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4015
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_45863"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            338..961
                     /locus_tag="VEA63_05300"
     CDS             338..961
                     /locus_tag="VEA63_05300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin"
                     /protein_id="HYD83441.1"
                     /translation="MKKYIVEFIGTFFLVFTVGLAVRQGAPLAPIAIGSVLMVMIFAG
                     GHISGGHFNPAVTLAAFLRGRCERRDVLPYWIAQFLAGLAAALLVNVVLDGRVDPGAV
                     ALHPTLNSFIVEFLFTFALCWVVLNTATAKATMGNSFYGLAIGFTVLVGAITVGGISG
                     GAFNPAVGLGVFTMGLESARQFGVYLISEFAGAAVAAFAYRKINGLD"
     gene            complement(1118..2797)
                     /locus_tag="VEA63_05305"
     CDS             complement(1118..2797)
                     /locus_tag="VEA63_05305"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009513111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HYD83442.1"
                     /translation="MLKKIFAKLRDSIGPSRASKPAASSGKPASSAKPHGSTTPHRGE
                     PRTERGHGHGAKSGGGGHAQGGKPQAGRDARSGGEGHGRREDRGERGDGRGGRGQGRS
                     SQQGGGRGGQGRGRFERGGPSDRTPAGRPRKDDSFDHPRATPSKPAVPVDVPKMDTEF
                     SKLGLSDALAYAVQEMGYVSPTPIQAQAIPVVLQGGDVIGSAQTGTGKTAAFALPIIQ
                     RLGAHGKLRCLILEPTRELALQVEEAFHKFAKFTDLRVTIVYGGVGYGKQTDDLRRGM
                     DILAATPGRLLDHLEQGNCSLENIDILVLDEVDRMLDMGFLPDVRRIVQKCPKARQTL
                     FFTATLPPELEQLAGWALHNPVKVEIGRQRSPAETVSHAFYPVVASQKFELLQLLLEQ
                     TEFKSVLIFSRTRMGADRIAHKLQRSGHTVGVMHSDRSQRERIEALDGFKSGKFEVLV
                     ATDIAARGLDIAGVSHVINYDVPENAEDYVHRIGRTGRAQNTGDAFTLVTEEDVRDAR
                     SIERYMGVTVERKKIEGFPYIYSALFDEKALAETAAAAVKPASRLHRGVRR"
     gene            2928..3359
                     /locus_tag="VEA63_05310"
     CDS             2928..3359
                     /locus_tag="VEA63_05310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009508790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HYD83443.1"
                     /translation="MTRNSRFAVSVHILAYLAYRAGSAVSSSEIAGSVDTNPVVIRRL
                     LSALVKARLVTAQKGATGGFSLACAPEEISLLDIYRAVHAQPSPALRHFAPNQLCPVG
                     ARIETIIGDLFAKAQASMEAELARATLSGINHQVQHACAAK"
     gene            complement(3443..3850)
                     /locus_tag="VEA63_05315"
     CDS             complement(3443..3850)
                     /locus_tag="VEA63_05315"
                     /inference="COORDINATES: protein motif:HMM:NF023514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3568 family protein"
                     /protein_id="HYD83444.1"
                     /translation="MKSRINMGAVRRIGFSVLLAAVVAGTSGCLAVAAGAGAGAAVAY
                     VRGELRVTLPSNYEATSRATNRAIEQLQFTKVSERKDALLDLIEARNAADKKIEIRLE
                     NTARELTKVRIRIGIFGDEALSMAILDKIEANL"
BASE COUNT          657 a         1513 c         1083 g          762 t
ORIGIN      
        1 ggccatttgc tccgccgtgt aacccagttt ctcgccgtag ttgtccgcat tctcaccgcc
       61 gcagcagccg ccggtcgcac agccacacga gtcgcccgcc tgctcggcaa tggcaccgta
      121 acgttcgcgg acatagccgc ggatttcctc attatccaga gatgtgattt tggtcatgat
      181 gaagaaggtt cactcaggca gacgcgaaag gtgtcgcccg gacgcaacgt ttctcgcccc
      241 ttcgcggcgc atcccgaatt cgccgtcgca cgcatgccgc gaaatcccat tggcctttgc
      301 cgcgcaaccg cctagcttcc ctgccccttc ctaccccatg aaaaaataca tcgtcgagtt
      361 catcggcacg ttcttcctcg tgttcaccgt cggtctcgcc gtgcgccagg gcgcaccgct
      421 cgctcccatc gccatcggct ccgttctcat ggtcatgatc ttcgccggcg gacacatttc
      481 cggcggacac ttcaatcccg ccgtgacgct cgccgcattc cttcgcggcc gctgcgaacg
      541 ccgcgacgtg cttccttatt ggatcgcgca atttctcgcc ggtctcgccg ccgcgctgct
      601 cgtcaacgtc gtcctcgacg gccgcgtcga tcccggcgcc gtcgcgctgc accccacgct
      661 caactccttc atcgtcgagt tcctcttcac cttcgcgctc tgctgggtcg tgctcaacac
      721 cgcgaccgcc aaagcgacca tgggaaactc cttctacggc ctggccatcg gcttcacggt
      781 gctcgtcggc gcgatcaccg tgggcggaat ttccggcggc gccttcaacc ccgctgtcgg
      841 cctcggcgtg ttcaccatgg gcttggaatc cgcccgccag ttcggcgtgt atttgatttc
      901 tgaattcgcc ggcgccgctg tcgccgcctt cgcttaccgc aagatcaacg gcttggactg
      961 acgcgccccg ccgctgcgcg gcggaaactc caagctccaa gcgcgcaagc tccagagaaa
     1021 ctccaaaatc caatcacgcc aaacttcgat agttgggcgc tggtgtttct ctggagcttg
     1081 gcgcttggat gttggagctt ccggcgcgaa gcgccggtca ccgccgcacg ccgcgatgca
     1141 accggctcgc cggcttcacc gccgccgcag ccgtctccgc cagcgctttc tcgtcgaaca
     1201 gcgccgaata aatatacggg aagccttcga ttttcttccg ctccaccgtc acgcccatgt
     1261 aacgctcgat ggaacgcgcg tcgcgcacgt cctcctcggt caccagcgtg aacgcatcgc
     1321 ctgtgttctg cgcccggccg gtccgcccga tgcggtgcac gtaatcttcc gcattctccg
     1381 gcacgtcgta attgatcacg tgagacacgc ccgcgatgtc caaaccacgc gccgcgatgt
     1441 ctgtcgccac gagcacctcg aacttgccgc tcttgaagcc gtccaacgcc tcgatgcgtt
     1501 cgcgctggct ccggtccgag tgcatcacgc cgaccgtgtg tccgctccgc tgcagcttgt
     1561 gcgcgatgcg gtccgcgccc atgcgcgtcc gtgaaaaaat caacacgctc ttaaattccg
     1621 tctgctcgag cagcagttgc agcaactcga acttctgcga cgccacgacc ggataaaacg
     1681 cgtgcgacac cgtctccgcc ggcgaacgct ggcggccgat ctccaccttc acgggattgt
     1741 gcaacgccca ccccgcgagt tgctcgagtt ccggcggcag cgtcgccgtg aagaacaacg
     1801 tctgccgcgc cttcggacac ttctgcacga tccggcgcac gtccggcagg aagcccatat
     1861 cgagcatgcg atcgacttcg tccagcacca ggatgtcgat gttttccagc gagcagttgc
     1921 cctgctccag atgatccaac aagcgccccg gcgtcgccgc caggatgtcc atcccgcgac
     1981 gcagatcgtc ggtctgcttg ccgtatccaa caccgccata cacaatcgtc acccgcagat
     2041 cggtgaactt cgcaaatttg tgaaacgcct cctcgacctg cagcgccagc tcacgcgtcg
     2101 gttcgagaat caggcagcgc aacttcccgt gcgcacccag ccgctggatg atcggcagcg
     2161 caaacgccgc cgtcttaccc gtgcccgtct gcgcggaccc gatcacgtcg ccgccctgca
     2221 acaccaccgg aatcgcctgc gcctgaatcg gcgtcggtga cacatagccc atctcctgca
     2281 ccgcataagc cagcgcatcc gacaaaccga gcttgctgaa ctccgtgtcc attttcggca
     2341 cgtcgaccgg caccgcgggt ttcgacggcg tggcgcgcgg atgatcgaaa ctatcgtcct
     2401 tccgcggccg gcccgccggc gtgcgatccg acggcccgcc gcgttcaaaa cgaccacgcc
     2461 cctggccacc gcgcccgccg ccttgttgac tggaacgccc ctggccgcgt ccaccgcgac
     2521 cgtcgccgcg ctcaccgcga tcttcgcgcc gaccgtgacc ctcgccgccc gatcgcgcat
     2581 cgcgcccggc ctgcggcttg ccaccctgcg catgaccgcc gccacccgac ttcgcgccgt
     2641 gaccgtggcc gcgttccgtc cgcggctcgc cgcgatgcgg ggtcgtggac ccatgaggtt
     2701 tggccgaaga agcgggtttg ccgctgctcg cagcaggctt ggaggctcgg gaaggcccga
     2761 tcgaatcgcg cagcttcgca aaaattttct ttaacatgag aacgttggga aaggtttaag
     2821 ctcgccgaaa ccccctcgtt tgcaatcccc gatataaccc ggccgcagct cgcagacctc
     2881 gccgtcgctt gaccgttttc ccaaggccga ttgggtaggc gagccccatg acccgcaact
     2941 cgcgcttcgc cgtcagcgtt cacattctcg cttacctggc ttatcgcgcg ggctccgccg
     3001 tctcgtcctc ggagatcgcc ggcagcgtcg acacgaatcc cgtcgtgatc cgccgcctct
     3061 tgtcggcact ggtcaaagcc cggctcgtca ccgcccaaaa aggcgcgacc gggggtttct
     3121 cgctcgcctg cgcgcccgag gagatctcgt tgctcgacat ctatcgcgcg gtccatgcgc
     3181 aaccctcgcc cgcgctccgc cacttcgctc cgaatcaact ctgccccgtc ggcgccagaa
     3241 tcgaaacgat catcggcgac ctgttcgcca aagcgcaggc cagcatggaa gccgagctcg
     3301 ctcgcgccac gctctccggc atcaaccatc aggtccagca cgcctgcgcc gccaaatagc
     3361 agcgcaacgc gctcctcgcg cgcgcatcac ggcccgcggc tcagctcgcg ctgccgcggc
     3421 atccgcgcgc ggccgcctcg cctcagaggt tcgcctctat cttgtcgagg atcgccatcg
     3481 agagcgcttc atcgccgaaa atcccgatac gaatccgcac cttcgtcagc tcgcgcgccg
     3541 tgttctccag gcgaatctcg atcttcttgt ccgcggcgtt ccgcgcctcg atcaagtcga
     3601 gcaacgcgtc cttccgctcg ctcaccttcg tgaactgcaa ctgctcgatc gcgcgattgg
     3661 tcgcgcgcga cgtcgcttcg taattcgatg gcagcgtcac ccgcagttcg ccgcgcacat
     3721 acgcgaccgc cgctccggcg cccgcgcccg cagccaccgc gaggcaaccc gacgtgcccg
     3781 ccacaaccgc cgccagcagc acgctgaacc cgattcgacg aacggcaccc atgttgattc
     3841 tggatttcat gttgagagtt ggacgcccca acagaccgcg agttcgctca aacgtttcat
     3901 ccgcgatcca cgcttggctt ggccagcgcc gcgcctcatg ttcgcgcgcg tttctcgttt
     3961 cccgcaccgc gccatgcatc acgacgtcct ttggctcgcc cgtctcgcca tcgat
//
LOCUS       DASPLT010000517         2472 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_293479, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000517 DASPLT010000000
VERSION     DASPLT010000517.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 2472)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2472)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2472
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_293479"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(238..597)
                     /locus_tag="VEA63_05320"
     CDS             complement(238..597)
                     /locus_tag="VEA63_05320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HYD83445.1"
                     /translation="MNTQKIADRLVTLCREMKWEAAQRELYAADVVSIEPYASPGFEK
                     ETRGLDAILEKGRKFDAMVETMHTLTVSDPLVADSSFACTMRMDATMKGKGRMDMTEL
                     CVYEVKDGKIVSEQFHL"
     gene            complement(950..>2472)
                     /gene="nhaA"
                     /locus_tag="VEA63_05325"
     CDS             complement(950..>2472)
                     /gene="nhaA"
                     /locus_tag="VEA63_05325"
                     /inference="COORDINATES: protein motif:HMM:NF018648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HYD83446.1"
                     /translation="TLTEDDLRAVTDEFVLAADAGAPTPEEALQAKARVDEDIGSARA
                     SGVRFTPTFFINGRRYDGLWEETEFTQAILGSLGHRVRSAALDFARWAPSTGLLLVLA
                     TVLAVVLTNSPFGSRFAAVWEMPFGFDFGEASFRMSLLHWINDGLLTIFFLVVGLEIK
                     REFTVGHLASWRAAALAVAAAIGGMAMPVLLYKLAIPTGNWSHGWGVPMATDTAFAIA
                     LIVMLGRRVPVELRIFLTAASIVDDIGAIIVVAIFYSAELNLGALALAGAATGMLALL
                     NRLGVYRVAPYALTGLVLWAAVHAGGLHATLAGVVLAMFMPTRRPPNLRAMMAQADAI
                     LISEARQSGDALRHGPSEPALEALDAIHDRLESPANRVLRTVSVRSNYFVLPLFALAN
                     AGVVFSMDVFHGHGPLMLAIGAGLVLGKPLGFVAACALAVKLKIAVKPAVYTWRQMAG
                     AGALAGIGFTMSLFIAGQAFPMESDFAAAKIAVFAASVVSAIVGVALLGSAKQTPR"
BASE COUNT          507 a          900 c          665 g          400 t
ORIGIN      
        1 caccggcgga aaatgaaaaa aagcggcaag gtcgcgtccc gcgaatgcgg cacccacgag
       61 gggcaacccg atcacgagga agaaaatcgc cgcgccccag gtccaacgaa gagtgcgcag
      121 cggacgcatg gccgcagcga aaccgcgtct cgtgcgttcg caacctcgcg caccgcgcac
      181 ctagctgagc cggcgtccgc gcggcccgta aacgcacgcc gttgaatact cgaccctcta
      241 caggtgaaac tgctccgaca caattttccc gtctttcacc tcgtagacgc agagttcggt
      301 catatccatc cggcctttgc ctttcatcgt ggcatccatg cgcatcgtgc aggcgaacga
      361 actgtccgcc acgagcggat cggacaccgt gagcgtatgc atggtctcca ccatcgcgtc
      421 gaacttccgg cctttctcca ggatcgcgtc gagcccgcgc gtttcctttt caaacccagg
      481 cgacgcgtaa ggttcgatgc tgacgacgtc cgcggcatac aactcgcgct gtgcggcttc
      541 ccatttcatt tcgcggcata aggtgacgag gcgatcggca attttttggg tgttcatgtt
      601 ggggcaactt cagggttaga acggtgtgaa atatacggcc gccactgacg agatcggcac
      661 tgactgcaat tttcatcctc ccgttccaac ggtttttcgc tccacgcaaa tgtccgccga
      721 agcgcggctc gttcgttgaa gcagcgaccg tgtgctcacg tgctgatcac cagcacgccg
      781 aaaacctcct gacagcaggt cgtcagtggc cgcggttact cttcgcccat gctcacgctt
      841 tttctcatcc tcgttgctct cgcttgtctc gtcgcagtcg acgccgaaag cgccagcccg
      901 cgcgactaac gtttcgccaa cctgacccga ggccgcggag atccgcccgt catctcggcg
      961 tctgcttcgc gctcccgagc aatgccacgc cgacgatcgc ggacaccacc gacgccgcaa
     1021 acaccgcgat cttcgccgcc gcgaaatcgc tctccatcgg aaacgcctgg cccgcgatga
     1081 agagcgacat cgtgaaaccg attcccgcca acgcgcccgc tcccgccatc tgccgccacg
     1141 tgtagaccgc cggcttcacg gcgattttca atttcaccgc gagcgcgcac gccgcgacga
     1201 atcccaaggg tttcccgagc accagccccg caccaatggc gagcatcaac ggaccgtgcc
     1261 cgtgaaacac gtccatcgag aacaccacgc ccgcgttcgc cagagcgaac agcggcagca
     1321 cgaaatagtt cgaacgcacc gacaccgtgc gcagcacgcg attcgccggc gactccaacc
     1381 ggtcgtgaat cgcgtccaac gcttccagcg ccggttccga cggaccatgc cgcaacgcat
     1441 ccccgctctg cctcgcttcg ctgatgagaa tcgcatccgc ctgcgccatc atcgcacgaa
     1501 gattcggcgg tcggcgcgtc ggcatgaaca tcgcaagcac cacgcctgcg agcgtcgcat
     1561 gcaacccgcc cgcgtgcacc gccgcccaca gcaccagccc cgtcagcgca tacggcgcca
     1621 cccggtaaac gcccaaccga ttcagcaacg ccagcatacc ggtcgccgcg ccggccagcg
     1681 cgagggcgcc caggttcaac tccgcggagt aaaaaatcgc gaccacgatg atcgcgccga
     1741 tgtcgtcgac gatggaagcc gccgtcagaa aaattctcag ctccaccggc acgcgccgcc
     1801 ccagcatcac gatcaacgcg atcgcaaacg ccgtgtccgt cgccatcggc acgccccacc
     1861 cgtgcgacca gttgcccgtc ggaatcgcca gcttatacag cagcaccggc atcgccatcc
     1921 caccgattgc cgcggcgacg gccagtgccg cggcgcgcca actcgcgaga tgcccgaccg
     1981 tgaactcgcg tttgatctcc aagccgacca ccaggaagaa aatcgtcagt agcccgtcgt
     2041 tgatccagtg gagcagcgac attcggaacg atgcttcgcc gaaatcgaaa ccgaacggca
     2101 tctcccacac cgccgcgaaa cgcgatccga atggcgaatt cgtcaacacc accgcgagca
     2161 ccgtcgccag caccagcagc aaaccggtcg acggagccca gcgcgcgaag tccaatgccg
     2221 ccgagcgcac gcgatggccg agcgaaccca ggatcgcctg cgtaaattcc gtctcctccc
     2281 agagtccgtc gtagcgccgg ccgttgatga aaaatgtcgg cgtgaaacga acgccgcttg
     2341 cccgggccga gccgatgtcc tcgtcgaccc gcgccttcgc ttgcagcgct tcttccgggg
     2401 tcggcgcccc ggcatccgca gccaacacga attcatctgt caccgcgcgc aaatcgtcct
     2461 ccgttaacgt gg
//
LOCUS       DASPLT010000518         1841 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_211285, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000518 DASPLT010000000
VERSION     DASPLT010000518.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1841)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1841)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1841
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_211285"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(187..927)
                     /locus_tag="VEA63_05330"
     CDS             complement(187..927)
                     /locus_tag="VEA63_05330"
                     /inference="COORDINATES: protein motif:HMM:NF025160.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HXXEE domain-containing protein"
                     /protein_id="HYD83447.1"
                     /translation="MHEVLVGWPWLSLAFAVLFLAWWFLRRRGTLPFRSRFTEPSWLL
                     WLPLPVYMLHQFEEHGVDLFGRHYHFQTDLCTALGYANVADCPATEVFICAVNVGTVW
                     IAGLVAGVLGPRRPIAAAGMIGLLAVNVVAHLGPFVRNGGAYNSGLLSAVVLFLPVVV
                     WLFRALLARGFFRRRDVLLGVAVGFLMHAVLLGSVILADKGLLSSSALVAIQIVNGFV
                     PLGISFLLERQPPSEARPESISSLTARR"
     gene            1221..>1841
                     /locus_tag="VEA63_05335"
     CDS             1221..>1841
                     /locus_tag="VEA63_05335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD83448.1"
                     /translation="MLSSFAMLTLQWYHELPEWQFCFIVVAVFIFVAVGGQMAIRPWV
                     RRWLGDGDYNDIVGHYLGAFGVLYGITLGLISVGAWENYGDVESKASAEAAALASLYR
                     NVDSYPEPARTELTALLRNYMRELIDVAWPEMRRGIVPRVGNPLILRFQKTLVAFEPM
                     TAAQKALHSESFYRFNQVTELRRLRIDCVEGRLPTILWVVMLAGSFM"
BASE COUNT          356 a          573 c          598 g          314 t
ORIGIN      
        1 ggcgcgtccg ttgcgacacg ttgcgtggcg cgcgcgcgat cacggcaggc aggcaggcag
       61 gcagggacgc ctgccgccac gcgcgagcac cgaatcggta ggtgaggcgc cactcgcgaa
      121 actcgcgtgg cgcgagggcg gcgatgagac ccggacgaag cgcggcctgc ctttcgatcg
      181 tgtctactac cgtcgagcgg tgagtgacga tatactctcg ggccgcgctt ccgacggcgg
      241 ctgccgctcg aggaggaagc tgattccgag cggcacgaaa ccgttgacga tctggatcgc
      301 cacgagcgcg gatgaagaga ggagcccttt gtcggcgagt atgaccgagc cgagcagcac
      361 cgcgtgcatc aggaatccca cggccacgcc gagcaggacg tcgcgccgac gaaaaaagcc
      421 tcgcgccagc agcgcgcgaa acagccacac gaccactggc aaaaacaaca cgacggcgga
      481 gagcaggccg gaattgtaag caccgccatt gcgcacgaac ggcccgagat gcgccacgac
      541 gttgaccgcg aggagcccga tcatccctgc ggcagcgatc ggacgccgcg gaccgagcac
      601 gcccgcgacc aagccggcga tccacacggt gccgacgttg acggcgcaga tgaagacctc
      661 ggtcgccggg cagtcggcga cgttcgcata accgagtgcg gtgcagagat cggtttggaa
      721 atgatagtgc cgcccgaaaa gatcgacgcc atgctcctcg aactgatgga gcatatagac
      781 aggcaacggc agccagagca gccatgacgg ctcggtgaag cgcgagcgga aagggagagt
      841 gccacggcgc cgcaggaacc accacgccag aaacagcacc gcgaaggcga ggctaagcca
      901 cggccagcca accaggacct catgcatgcg ccgaccttgc ggggaaattt ccccggcgca
      961 cgcccgaatt cacggacact gaacgcgcga aaaaacttgg ttggaatcgc tcgattgcgt
     1021 ctgggccgtc gataggtgga ccgcctcgat tgagcagtgc cggccgatcg gctcatcccg
     1081 ttccgctccg tcgagacaaa gttctcacat ggatcgacgg gcggtgaggt tcgattccat
     1141 gcctcacccg cgcgacgcat gtcgcgtttc aacctcgctt tagcggtggg ctgtcgccct
     1201 cctgcttcgc tctttcgtcc atgctttcct ccttcgccat gttgaccttg cagtggtatc
     1261 acgagctacc cgaatggcag ttctgcttca tcgtggtggc cgtgtttatc ttcgtcgcgg
     1321 tgggcgggca gatggcgatt cgtccctggg tgcggcgctg gttgggcgac ggcgactaca
     1381 atgatattgt cgggcattat ctcggcgcgt tcggcgtgct ctacggcatc accctcggtc
     1441 tgatctcggt cggtgcctgg gagaattacg gcgacgtcga aagcaaggcc agcgcggagg
     1501 ccgcggcgct cgcttcgctc tatcgcaatg tggactccta tccggagccg gcacgcacgg
     1561 agctgacggc tctactgcgc aattacatgc gtgagctcat tgacgtcgcg tggcccgaga
     1621 tgcgtcgcgg catcgttcct cgcgtcggga acccgctgat cttacgtttc cagaaaacgc
     1681 tggtggcctt cgaaccgatg accgcagcgc agaaggcgct ccactccgaa tcgttctatc
     1741 gcttcaacca ggtgaccgaa ctgcgccggc tgcgcattga ctgtgtggaa ggccgtttgc
     1801 cgacgattct gtgggtggtg atgctggccg gctcgttcat g
//
LOCUS       DASPLT010000519         1625 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_230997, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000519 DASPLT010000000
VERSION     DASPLT010000519.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1625)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1625)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1625
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_230997"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..872)
                     /locus_tag="VEA63_05340"
     CDS             complement(<1..872)
                     /locus_tag="VEA63_05340"
                     /inference="COORDINATES: protein motif:HMM:NF004845.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HYD83449.1"
                     /translation="MKTKQEPINSGYGLRTEAREALGGRDLTGKVAIVTGGYSGIGLE
                     TTKTLAGAGAIVIVPARSAEKAQKALVGIANVEQAALDLADPGSIDAFAGGFLSRTKT
                     LDILINNAAIMASPLMRDARCYEAQFATNHLGHFQLAARLWPALKAANDARVVALSSI
                     GHRIAPPDLDDPNFERTEYNKWLAYGRAKSANALFAVGLDQRGAAHNVRAFAVHPGGI
                     MTDLQRYMPEEEQRAMGWIDEAGNVNERFKTPSQGAATSVWCAANAQLDGKGGVYCED
                     CDIAAAVPADDKGF"
BASE COUNT          258 a          509 c          528 g          330 t
ORIGIN      
        1 ctgaagcctt tgtcgtccgc gggcacggcg gccgcgatgt cgcaatcttc gcaatagaca
       61 ccgcctttac cgtcaagctg cgcgttcgcc gcgcaccaga cgctggtggc ggcgccttgg
      121 cttggcgtct tgaagcgctc gttgacgttg cccgcttcgt cgatccagcc catcgcacgc
      181 tgttcttctt ccggcatgta acgctgcagg tcggtcatga tgccgccggg atggacggcg
      241 aaggcgcgca cgttgtgcgc tgcgccgcgc tggtcgaggc cgacggcgaa cagcgcgttg
      301 gcggatttgg cgcggccgta ggcgagccat ttattatatt cggtgcgctc gaagttcgga
      361 tcgtcgagat cgggcggcgc gatgcgatgg ccgatcgacg agagcgcgac cacgcgggcg
      421 tcattcgccg ccttcagcgc cggccacagc cgcgccgcca gctggaagtg gccgaggtga
      481 ttggtggcga attgcgcttc gtagcatcgc gcatcgcgca tcagcggcga ggccatgatg
      541 gcggcgttgt tgatcaggat gtcgagtgtc ttggtgcgcg agagaaagcc cccggcgaac
      601 gcgtcgatgc tgccgggatc ggcgagatcg agcgccgcct gctccacatt ggcgatgccg
      661 acgagcgcct tctgcgcttt ctccgcgctg cgcgcgggca cgatgacgat cgcgccggcg
      721 ccggcgagcg tcttcgtcgt ctcaaggccg atcccggaat agccgccggt gacgatggcg
      781 accttgccgg tgagatcgcg cccgcccagc gcctcgcgcg cctcggtgcg caggccgtag
      841 cctgaattga tcggctcttg tttcgtcttc attggccgca tctcctctcg cagcaggcta
      901 aacttaaaga gcgcgcacgc gccagtgtga ttgcggcgca caacgggcgc gcgccggcgc
      961 cgtgcgggcc tgaaggcccg cggtccaact tttcaatatt cgctcccgaa gggaggcggg
     1021 gcgcgtcgga cgcgctgagg tagtctctag tgtgcgccgg tcatcgctga ccaacgcgtc
     1081 gaacgcgccc cgtaaacgtt cgttctttcc cggatgccgg tacaggactt tcggtgggac
     1141 tctgtccagc tgatccggtc cggtgcagtc ctcgtctctt gcgagacaat tggctggtcc
     1201 cggccgaggc ttagatccac ccggcattac cggttccgtg tgcaagattt ctccgagaaa
     1261 ccttgggcct cgcggtttgc atccccagcg agcaccgaac gccgctctga catttcaagg
     1321 ttcggggctc tcgaaatcct cccgtgccag agcgaggcat tatacgccaa gcgcaaggcc
     1381 agtcggattg attttctccg gagtgttgat ttccaacgtg ttgcgcggga aaacgcatgg
     1441 atagaggaag gttctatcct cgcattcgcg ctaggcgccg acagagtgtc acccatctat
     1501 ccgggctaaa ctgtcacctg aattgtggct ggacactatc ttgtacaatc gcctttgcgc
     1561 caagccccgg gaatggtgtg ttcgtctagc gcgcggcgat cagtgtcatc gccagcgccg
     1621 gatcg
//
LOCUS       DASPLT010000520         1254 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_107798, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000520 DASPLT010000000
VERSION     DASPLT010000520.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1254)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1254)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1254
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_107798"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..425
                     /locus_tag="VEA63_05345"
     CDS             <1..425
                     /locus_tag="VEA63_05345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="[acyl-carrier-protein] S-malonyltransferase"
                     /protein_id="HYD83450.1"
                     /translation="QIIVSGEKTKVEAAVAGAKDRGIKKVMLLNVAGAYHSRLMEPAR
                     AEFAAFLDGVKFAAPKFTVFTNTTGQAVSEPAAIKEALVKQVVSSVLWEDCMRSAAAA
                     GATEFWELGPAGVLAGLARRTEKSWNVKSFAEIGDVTA"
     gene            430..>1254
                     /locus_tag="VEA63_05350"
     CDS             430..>1254
                     /locus_tag="VEA63_05350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HYD83451.1"
                     /translation="MSYENTRNFCIIAHVDHGKTTLSDRLLEHTNQVAMRVMTDQHLD
                     SMDLEKERGITIKSHPVTMTYPAKDGKIYKLNLMDTPGHVDFSYEVSRSLAACEGAVL
                     LIDAAQGVEAQTVANAHLAFAQKLRVIPVINKIDLPSANLDLCLKQLEDILTIPAEEA
                     ILASGKAGIGIEDILEAVVQRVPPPRWADHPHTRALVFDSLYDSYRGVIAYVRVFSGV
                     MKAGDMMMLMSNGQKSEVKEVGVFTPKMSKIASLGAGDVGYIVSSIKDTSEIKTGDT"
BASE COUNT          264 a          370 c          400 g          220 t
ORIGIN      
        1 ggcaaatcat cgtgtcgggc gaaaagacga aggtcgaagc ggcggtcgca ggcgcgaaag
       61 atcgcggcat caagaaagtg atgctgctca atgtcgcggg cgcttatcac agtcggctga
      121 tggagccggc tcgcgcggaa ttcgcggcgt ttctcgacgg cgtgaagttc gccgcgccga
      181 agtttaccgt tttcaccaac acgaccggcc aggccgtcag tgagccggcg gcgatcaagg
      241 aggcgctcgt gaagcaagtc gtgtcctcgg tgttgtggga ggattgcatg cgcagcgcgg
      301 cggcggcggg cgcgacggag ttttgggaac tcgggccggc gggcgtgctc gccgggctcg
      361 cgcgacgcac cgaaaaatcc tggaacgtga aaagttttgc cgaaatcggc gatgtgactg
      421 cctgagccga tgtcctacga aaacacccgc aatttttgca ttatcgccca cgtcgaccac
      481 gggaagacca cgctgtcgga ccggttgctg gagcatacca accaggtcgc gatgcgcgtc
      541 atgaccgacc agcatctcga ctcgatggat ctcgagaagg agcgcggcat cacgatcaag
      601 tcgcatccgg tcacgatgac gtatccggcg aaggacggaa aaatctacaa gctgaacctc
      661 atggacacgc cgggccacgt ggacttctcc tacgaagtct cgcgcagtct cgcggcgtgc
      721 gaaggcgcgg tgctgttgat cgacgccgcg caaggcgtcg aagcgcaaac cgtggccaac
      781 gcgcacctgg ccttcgcgca gaagctccgc gtcattccgg tgatcaacaa aatcgatctg
      841 ccgagcgcga acctcgatct ctgcctgaag cagctcgagg acatcctcac gattcccgcc
      901 gaggaggcga ttctcgccag cggcaaggcc ggcatcggca tcgaggatat tctcgaggcg
      961 gtcgtgcagc gcgtgccacc gccgcgttgg gcggatcatc cgcacacgcg tgcgctcgtc
     1021 ttcgattcgc tctacgattc ctaccgcggc gtcatcgctt atgtgcgcgt gttctccggc
     1081 gtgatgaagg ccggcgacat gatgatgctc atgagcaacg ggcaaaaatc cgaagtgaaa
     1141 gaagtcggcg tgttcacgcc gaaaatgagc aagatcgcgt cactcggcgc cggtgacgtt
     1201 ggttacatcg tttccagcat caaggacacg tccgagatca agaccggcga cacg
//
LOCUS       DASPLT010000521         4233 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_149545, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000521 DASPLT010000000
VERSION     DASPLT010000521.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 4233)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4233)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4233
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_149545"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..772
                     /locus_tag="VEA63_05355"
     CDS             <1..772
                     /locus_tag="VEA63_05355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HYD83452.1"
                     /translation="KIVNPSDDAAGLAVSEKLDAQNKRVQAATTNVQNAVSYIQTADG
                     FMSGMTKILSRMSELAILAKDVTKNPTDIDLYQQEFTALQDQLRATIGGSTVEIGGTA
                     ITTPLGAFNGITLFGSNSAGLTVTIGQAVGQDMTIRESNLRAGASAALITQNASGVYN
                     VGIADVATIGHITDAIQQIASERAKLGASQSRLELASTTLSVEFENLSSAISRIRDVD
                     VAKESTQYAKYNILVQSGTAMLAQANQSPSAVLKLLQS"
     gene            complement(1022..>4233)
                     /locus_tag="VEA63_05360"
     CDS             complement(1022..>4233)
                     /locus_tag="VEA63_05360"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HYD83453.1"
                     /translation="HERLNAVLEQQAARLQELVARRTSELRTLSVNLRTTTRENAQLG
                     LVARNTTNGVVITDAEGHLEWANTAFERMTGYTFAEVKGRKPGHFLQGPDTDPASVAQ
                     LRRAVQSGERCYVEILNYTKSRHPYWQIVDMEPVHDAHGKLVNWIAIQTDVTAHRLAE
                     QHLRVLNERLQLATQSAALGVWEWDAAGQKHTWDERTHAIHGMKPGEFDGSFEAWMSC
                     LHPDDRPLATARFELVLAGGNFYDQEFRVTRGTDGTVRYVESRAIVQRDAQSKLLRIT
                     GTSRDVTAERENEERLRTLTDRWQLALRASRYGVWDWDPATGRLIWDERMFDVYGVNR
                     DTFPSTHQAWLELLHPDDRNRAEQIEQDALRFGREEFDNEFRIVLPDGFVRYVETHGY
                     IRRDETGQPVRIVGMDRDITAEKQTLEALRMAEERWQLAVEGTNDGVWDWNVPTGVTY
                     HDQRWSQMLGYETGEIPDTIESWRNLIHPDDLPGCVAATDDHFARRTALYHHEYRMRT
                     RQGDWKWILDRGKVVTRDAQGRPLRMVGMHTDITARKNLEQRLHQFEQLATQVSELAQ
                     IGGWELELDTMQLTWSEQVRRIHEVDQHFRPTVHSSIEFYAPESRDVMSSALKAAMAY
                     GRDYDLELPLITATGRRIWVRALTRAEFRDNKPVRLIGALQDISSRRDSEDARRKLEF
                     QLFQAQKMETLGTLAGGIAHDFNNLLTGIMGYQELVADVLGDNSIAADYLAQARGASL
                     RARDLVEQILTFGRQSPDDQHAALDMALVIEEARRLLRATIPATIAIETHVDPDCGPV
                     LGDATQIHQVLLNLGSNAAHAMHTKGGTLTISLAPVDREVSRSATLATLPAGDYLRLT
                     VSDNGHGMDETTLQRIFDPFFTTKEVREGTGLGLAVVHGIVRAHRGAIDVESRLGGGS
                     TFHIYLPTAAKDAEQEEREVLQSPRGAGEMIFIVDDEDTVASFTKFALENKGYRAATV
                     DTAAQCLEALRADPTACAALVTDQTMPGMTGTELAGKVREFAPNLPIIVMSGYFLKIP
                     PQDIAQIGHVELLGKPFTTDELAWMVHRALHPDPETAEV"
BASE COUNT          715 a         1470 c         1248 g          800 t
ORIGIN      
        1 gaaaatcgtc aacccctccg atgacgccgc gggcctcgcg gtctcggaga aactcgatgc
       61 ccaaaacaag cgcgtccaag ccgcgaccac gaacgtccaa aacgccgtct cctacatcca
      121 gaccgccgat ggcttcatga gcggcatgac gaagatcctc tcgcgcatga gcgagctcgc
      181 gatcctcgcg aaggacgtca cgaagaaccc gacggacatc gatctttatc agcaggagtt
      241 caccgcgttg caggaccagc tccgcgcgac catcggtggt tccaccgtcg agatcggcgg
      301 caccgccatc accacgccgc tcggcgcgtt caacggcatc acgcttttcg gctccaacag
      361 cgccggcctc accgtgacca tcggccaggc cgtcggtcag gacatgacca tccgcgagtc
      421 caatctgcgc gccggcgcgt ccgccgccct catcacccaa aacgccagcg gcgtctataa
      481 cgtcggcatc gccgacgtgg cgaccatcgg ccacatcacc gacgcgattc aacagatcgc
      541 ctccgaacgc gcgaagctcg gtgcctcgca atcgcgcctc gaactcgcct caaccacgct
      601 gagcgtcgaa ttcgaaaatc tctcgtccgc catctcgcgc atccgcgacg tggatgtcgc
      661 caaggagtcc acgcaatacg cgaaatacaa catcctcgtg cagtccggca ccgcgatgtt
      721 ggcacaggcg aatcaaagtc cgtccgcggt tttgaagctc ctgcagagct agtttttggg
      781 gtagggtcgt ttcataaaaa aagggcgggt catcgacccg ccctttttgc gtcacaaaac
      841 ggagtccttc tggttttccg tatcgctcct ttcggccgcg ctcgcggacc gcgggagcta
      901 acgcgcaaaa ttctatcgta gtgaaaagcg gagcttttcg gctctcgcct acacgaaacg
      961 ctcacgcgtt tcgctaaaac gtgctgcgcg cacctcgcgg cggaaatgtg aattttgcag
     1021 gctaaacttc cgccgtttcc ggatccggat gcagcgcgcg atgcaccatc cacgcgagct
     1081 cgtcggtggt gaacggtttt cccaacagct ccacgtggcc gatctgcgcg atatcctgcg
     1141 gcggaatttt caggaaatat cccgacatca cgatgattgg cagattcggc gcgaattcgc
     1201 gcactttccc ggccagctcc gtgccggtca ttcctggcat cgtctgatcc gtcaccagcg
     1261 ccgcgcaggc ggtcgggtcg gcacgcaacg cctccaggca ctgcgccgcc gtatccaccg
     1321 tggcagcacg gtagcctttg ttctccaatg cgaatttcgt aaagctcgcc accgtgtcct
     1381 cgtcgtcgac gatgaagatc atctcgcccg cgccgcgcgg cgattgcagc acttcgcgct
     1441 cctcctgctc ggcgtccttc gccgccgtcg gcagataaat gtggaacgtc gaaccgccgc
     1501 ccagccggct ctcgacatcg atcgcgccgc gatgggcccg cacaatgccg tgcaccaccg
     1561 cgagtccgag gcccgtgcct tcgcgcactt ccttggtcgt gaaaaacgga tcgaagatgc
     1621 gctgcaaggt cgtctcgtcc atgccgtgcc cattgtcgct cacggtcagt cgcaggtagt
     1681 cgcccgccgg cagcgtcgcg agcgtggcgc tgcgcgagac ttcgcgatcg acgggcgcca
     1741 gactgatcgt gagcgtgccg cccttggtgt gcatcgcgtg cgcggcgttc gagccgagat
     1801 tgaggagcac ctgatgaatc tgtgtcgcat cgccgagcac cggtccgcag tccggatcga
     1861 cgtgagtttc gatcgcgatc gtcgccggga tcgtggcgcg caacaaccgc cgcgcctcct
     1921 cgatcaccag cgccatgtcg agcgcggcat gctggtcgtc cggcgactgc cgcccgaacg
     1981 tcaggatctg ctccaccaaa tcgcgtgcgc gcaaactcgc tccgcgcgcc tgcgcgagat
     2041 aatccgccgc gatcgagttg tcgccgagca cgtccgccac gagctcctga tagcccatga
     2101 tgcccgtgag cagattgttg aaatcgtgcg cgatgccgcc cgcgagcgtg ccgagcgttt
     2161 ccatcttctg cgcctggaac aactggaact ccagcttgcg ccgcgcatcc tcgctgtcgc
     2221 gccggctgct gatgtcctgt aacgcgccga tcagccgcac cggcttgttg tcgcggaact
     2281 ccgcccgcgt gagcgcgcgc acccagatcc gccgtcccgt cgccgtgatc aacggcagtt
     2341 cgagatcgta gtcgcgaccg taagccatcg ccgccttcaa cgcgctcgac atcacatcgc
     2401 gcgactccgg cgcgtagaac tcgatcgacg aatgcaccgt gggccggaaa tgctgatcca
     2461 cttcgtggat acggcggacc tgctccgacc acgtgagctg catcgtgtcg agctccagct
     2521 cccagccgcc gatctgcgcg agctcgctga cctgcgtcgc cagctgctcg aactggtgca
     2581 accgctgttc gagattcttg cgcgccgtga tgtccgtatg catgccgacc atgcggagcg
     2641 gccggccttg cgcatcgcgt gtcacgacct tgccgcgatc gagaatccac ttccagtcgc
     2701 cctggcgcgt gcgcatccga tactcgtgat gataaagcgc cgtgcgccgg gcgaaatgat
     2761 cgtcggtcgc cgccacgcag ccgggcaggt cgtccggatg aatcaggttg cgccagctct
     2821 cgatcgtgtc gggaatctcg cccgtttcat agcccagcat ctgtgaccag cgttggtcgt
     2881 gataggtcac gccggttggc acgttccaat cccacacgcc gtcattcgtg ccctcgaccg
     2941 cgagctgcca ccgttcctcg gccatgcgca acgcttcgag cgtttgcttc tccgcggtga
     3001 tgtcgcggtc catgcccacg atgcgcaccg gctgaccggt ctcgtcacgg cggatgtagc
     3061 cgtgcgtctc gacgtagcgc acgaagccat cgggcagcac gatccgaaac tcgttgtcga
     3121 attcctcgcg tccgaaccgc agcgcatcct gctcgatctg ttcggcgcga ttgcggtcat
     3181 ccggatgcaa caactcgagc cacgcctgat gcgtgctcgg gaacgtgtcg cggttcacgc
     3241 cgtaaacatc gaacatccgc tcgtcccaaa tcagccggcc cgtcgccgga tcccaatccc
     3301 agacgccgta gcgcgacgcg cgcaatgcga gctgccagcg atcggtgagg gtgcgcagcc
     3361 gctcttcgtt ttcgcgctcg gcagtgacgt cgcggctcgt gcctgtgatc cggagcagct
     3421 tgctttgcgc gtcgcgctgc acgatcgcgc gcgactcgac gtagcgcacc gtgccgtcgg
     3481 ttccccgcgt gacgcgaaac tcctggtcgt agaaattccc gcccgccagc acgagttcga
     3541 agcgcgcggt cgcgagcggc cggtcgtccg gatgcaggca gctcatccac gcttcgaagc
     3601 tgccgtcgaa ctcgccgggc ttcatgccgt gaatcgcatg cgtgcgctcg tcccacgtgt
     3661 gcttttgccc cgccgcatcc cattcccaca cgccgagcgc cgcggattgc gtcgcgagct
     3721 gaagccgctc gttcaacacc cgcaggtgct gttcggccag acggtgcgcg gtgacgtcgg
     3781 tctggatcgc gatccagttg acgagtttcc cgtgcgcatc gtgcacgggc tccatgtcga
     3841 cgatctgcca atacggatgc cggctcttcg tgtagttgag aatctccacg tagcagcgct
     3901 cgccgctttg caccgcgcgg cgcaactgcg cgacgctcgc cggatccgtg tccggcccct
     3961 gcagaaagtg tcccggcttg cggcctttca cctccgcgaa ggtgtagccc gtcatgcgct
     4021 cgaacgcggt gttcgcccac tcgagatgtc cctccgcgtc ggtgatgacg acgccgttcg
     4081 tggtgttgcg cgcgacgaga ccgagctgcg cgttttcccg cgtcgtcgtg cgcaagttga
     4141 cgctcaaggt ccgcaactcg ctcgtgcgcc gggcgacgag ctcctgcagg cgcgcggcct
     4201 gttgctcgag cacggcgttg agccgttcgt gtt
//
LOCUS       DASPLT010000522         4636 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_88534, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000522 DASPLT010000000
VERSION     DASPLT010000522.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 4636)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4636)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4636
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_88534"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            5..169
                     /locus_tag="VEA63_05365"
     CDS             5..169
                     /locus_tag="VEA63_05365"
                     /inference="COORDINATES: protein motif:HMM:NF016432.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HYD83454.1"
                     /translation="MPDIPKKILAMYYFENMRLAEIAAVFGLTESRICQIHAQTILGL
                     RGYLQRVRNR"
     gene            297..1160
                     /gene="motA"
                     /locus_tag="VEA63_05370"
     CDS             297..1160
                     /gene="motA"
                     /locus_tag="VEA63_05370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor stator protein MotA"
                     /protein_id="HYD83455.1"
                     /translation="MLILIGALVVFVSTIGGFMLAGGSPLVLLHVSEFVVIGGVAIGV
                     LIIASPSHVLKEVMHKVMSCISGKNAGKTEFFELLKLLYEIFMVGRRNGLIALEEHVM
                     DPQKSAIFQRYPSLLQHPERLEFLVNGLKPVIDGKIKPDQLEELMTAEIDAKAEEWDH
                     PVHILQLVGDSLPGIGIVAAVLGIINTMSAIADGPEAVGHKVAAALTGTFLGIFFAYG
                     FVNPLANRIKFNNASELLCLRCIMQAVAGFAKGLAPLTAVEIARRSLDSSVQPGSSEL
                     ETALKALPPVK"
     gene            1286..2095
                     /locus_tag="VEA63_05375"
     CDS             1286..2095
                     /locus_tag="VEA63_05375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009507965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HYD83456.1"
                     /translation="MARKKAAAHHGGAWKVAYADFVTAMMALFMVLWISAQDQKILIA
                     TSQYFQNPFRSPMDATSGVLPFNTEKKTQSQGENEGSEKQDTKKKQIEMTFLNTVAAD
                     FYRLLHIDQQLTQKPIDIQVTSDGLRITLFDRATKPLFVGNTAEFSEWGTFVMQNLSW
                     SIERHGFRVTIDGHTKARLDLGRDDYSSWELSADRANAARRSLVRYAVDPSMIERITG
                     YADTRPLPNEDTQSESNQRITLSLAMSAKSREPAKTTVEQAARAAQPQPAS"
     gene            2092..2340
                     /locus_tag="VEA63_05380"
     CDS             2092..2340
                     /locus_tag="VEA63_05380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD83457.1"
                     /translation="MNSFLQGKKTLTIPPLRPVVGPPRPAIAPAGASSARPAAPVNCG
                     GASPSVEVVKEGDKVVRLVVVCGCGERVEIECLYPGGG"
     gene            2729..3478
                     /locus_tag="VEA63_05385"
     CDS             2729..3478
                     /locus_tag="VEA63_05385"
                     /inference="COORDINATES: protein motif:HMM:TIGR03506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body protein"
                     /protein_id="HYD83458.1"
                     /translation="MNIGMYQSAASLSALERWQDSVAQNITSSQVSGYRKRTINFSTQ
                     AAGELQSDPRRPVGNEGGIPTLFPKVNTGINFTSGETQPTRRELDVAIQGEGFFELLR
                     PDGSHVFSRSGEFRMRADRTLVTASGEEVLSTAGTPIIFVPNGGAISVNLDGSIFQGE
                     TPLGRLSVQRFADNAQLIPTSGGYFTAPEGLDPEPVDEPELLQGYLEGSNVTPLREMV
                     DLVLISRAYEANQKMINTLDQQMEKALNALG"
     gene            3685..4470
                     /gene="flgG"
                     /locus_tag="VEA63_05390"
     CDS             3685..4470
                     /gene="flgG"
                     /locus_tag="VEA63_05390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007363293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009426 - bacterial-type flagellum basal
                     body, distal rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HYD83459.1"
                     /translation="MNLSLYSAATGMEAQQLNLNTIANNLANVNTPGFKRSKIEFQDL
                     LYQKPTRASGTDSGGGNIVPTGIEVGNGSRVASTSKVFTQGQLTSTGEKLDIAIQGDG
                     FFEVQRPDGTIGYTRDGSFKLNAQGQVVTIDGLPVLSGFQPIPPGASGVSIAENGQVT
                     VQSSTGSTTFRLSLTRFANPSGLRSMGGNLYEETAASGTPEIGQPGELGFGSLMQGYI
                     ESSNVNIVEEMVNLIVAQRAYEINSKSIQTSDEMLQNIAQMKR"
BASE COUNT          921 a         1570 c         1270 g          875 t
ORIGIN      
        1 cgaattgccc gatataccga agaagatcct cgcgatgtat tatttcgaaa acatgcgcct
       61 cgccgaaatc gccgccgtct tcggcctgac ggagtcgcgc atctgccaaa tccacgctca
      121 aaccattctc gggctgcgcg gttaccttca acgcgtccgc aatcgctgac gcggaagcac
      181 cagattccaa catccaaact ccaaagaaac ttcaaatccc aacgtccaat gaagctgtct
      241 gaatctggga tttctctgga gctcggtgct tggagcttgg tgtttcgccg ttcgccatgt
      301 tgatcctgat cggagctctc gtcgttttcg tgtcgaccat cggcggcttc atgctcgccg
      361 gcggctcgcc gctcgtgctg ctgcacgttt ccgaattcgt cgtcatcggt ggcgtcgcga
      421 tcggcgtgct catcatcgcc agcccttcgc atgtgctgaa ggaggtcatg cacaaggtca
      481 tgtcgtgcat ctcgggcaag aacgccggca agacggagtt cttcgaactg ctgaaacttc
      541 tctacgagat tttcatggtc ggccgccgca acggactcat cgcgctcgaa gagcacgtga
      601 tggacccgca aaaaagcgcg atcttccaac gctacccgtc gctcctccag caccccgaac
      661 ggctggagtt tctcgtcaac ggcttgaaac ccgtcatcga cggcaagatc aagcccgacc
      721 agctcgagga gctgatgacc gccgaaatcg atgcgaaagc cgaggagtgg gatcacccgg
      781 tgcacattct tcaactcgtc ggcgattcgc tgcccggcat cggcatcgtc gccgccgtgc
      841 tcggcatcat caacacgatg tccgcgatcg ctgacggtcc cgaagccgtg ggccacaagg
      901 tcgccgccgc gctcaccggc acgttcctcg gtattttctt cgcttacggc ttcgtcaatc
      961 cgctcgcgaa tcgcatcaag ttcaacaacg cctccgagct gctctgcctc cgctgcatca
     1021 tgcaagcggt cgccggtttc gccaaaggcc tcgcgccgtt gaccgccgtc gagatcgctc
     1081 gccgttcgct cgacagctcg gtccagcctg gctcgagcga actcgagacc gcgctcaaag
     1141 cgctgccacc ggtcaagtga gcgtctgatg aagctgagag ctgagagtcc agagctgaga
     1201 gccgatcacc caatcgagaa gggtgttttg gcatttttgt gctctcagct ctggactctc
     1261 ggctctcagc tttccgtcca ttcccatggc taggaaaaaa gcagcagccc accacggcgg
     1321 cgcctggaag gtcgcttacg ccgacttcgt caccgcgatg atggcgctct tcatggtgct
     1381 gtggatctcc gcgcaggatc agaaaatcct catcgccacg tcgcagtatt ttcaaaatcc
     1441 gttccgctcg ccgatggatg cgacttcggg ggtgctcccg ttcaacaccg agaagaaaac
     1501 gcagagccag ggcgagaacg agggtagcga aaaacaggat acgaaaaaga agcagatcga
     1561 gatgacgttc ctcaacaccg tcgccgccga tttctaccgt ctcctgcaca tcgatcagca
     1621 gctcacgcaa aaaccgatcg acatccaggt gaccagcgac ggcctgcgca tcacgctgtt
     1681 cgatcgcgcc acgaaaccgc tctttgtcgg caacaccgcc gagttcagcg agtggggcac
     1741 gttcgtgatg cagaatctct cgtggtcgat cgagcgtcac ggttttcggg tgacgatcga
     1801 cggccacacg aaggcccgcc tcgatctcgg tcgtgacgac tattcctcct gggaactctc
     1861 cgccgatcgc gcgaacgccg cgcgccgcag cctcgtccgc tacgccgtcg atccttcgat
     1921 gatcgagcgc atcaccggct atgccgacac gcgtccgctt ccgaacgaag atacccagag
     1981 cgagtcgaac cagcgcatca cgctgagcct cgcgatgtcc gcgaaatcgc gcgagcccgc
     2041 gaaaaccacc gtcgaacagg ccgcgcgcgc cgcgcagccc cagcccgctt catgaactct
     2101 tttctgcaag gcaagaaaac cctcaccatt cctccgcttc gtccggtggt cggacctccg
     2161 cgtcctgcga tcgccccagc cggcgcgtcg tctgcgcgtc cggccgcacc agtgaattgc
     2221 ggcggcgcga gcccatcggt cgaggtcgtc aaggaaggcg acaaagtcgt ccgcctcgtc
     2281 gtcgtgtgcg gctgcggcga acgcgtcgag atcgaatgcc tctatcccgg cggcggctga
     2341 gcctgtcgga tcgcgatccg attctgcacg gcgtctgctg ggagtagtca cgtccgcgcg
     2401 cttatgtcgc gcgcacgctc gtgcgtgcaa tcccgctacg agatcggagg ggaggagcct
     2461 gcttgccggc gattcacgcc tcgcatgccg acgacgcacc gattgcctgc aagcaggctc
     2521 ctgcaattgg ctgcaattca ttgcaccgct ccaacgcctt tcgctcgcct ctgcgcaacg
     2581 cgacgcattg gcaacgccgc ccttcgccgc ggaaaatcct gccgcgaatt ttacggcaag
     2641 ttttgccaga tcgattgtgt gaaactgtat tacgcgagca caacgcgagt tatgcacttg
     2701 gcatcggctt tgctaacctt ccgtgtccat gaatatcggc atgtatcaaa gcgcagcttc
     2761 tctgtccgcc ctcgagcgct ggcaggactc tgttgcccag aatatcacgt cgagtcaggt
     2821 cagcggctac cgcaaacgca cgatcaactt ctccacgcag gcggcgggcg aattgcagag
     2881 cgatccgcgt cgtccggtcg gcaacgaagg cggcattccg accctctttc ccaaggtcaa
     2941 caccgggatt aatttcacga gcggcgaaac gcagccgacg cgtcgcgaac tcgatgtcgc
     3001 gattcaaggc gaaggtttct tcgaactgct gcggcccgat ggctcgcacg ttttcagtcg
     3061 cagcggcgag tttcgcatgc gcgccgatcg cacgctcgtc accgcaagcg gagaggaagt
     3121 tctcagcacc gcgggcacgc cgatcatttt cgtcccgaac ggcggcgcga tcagcgtcaa
     3181 tctcgacggt tcgattttcc agggcgagac gccgctcggc cgtctttccg tgcagcgctt
     3241 cgccgacaac gcgcaactca ttccgacgtc cggcggctat ttcaccgcgc ccgaaggtct
     3301 cgatcccgag cccgtcgacg agcccgagct gctccaaggc tatctcgaag gcagcaacgt
     3361 cacgccgctc cgcgaaatgg tcgacctcgt gctcatctcg cgcgcctacg aggccaacca
     3421 gaaaatgatc aacacgctcg atcaacagat ggagaaagcg cttaacgcgc tcggttgatc
     3481 caccgtgttt ttcgcaacgc gcacgcacgc ccgttccatg aattccccag acagacaacc
     3541 gcaatcacga ctacatggcc gagcccgcta tgggggcggg ccggccccaa tttctagccg
     3601 agagccaaga gcccagagcc aagagctcgg gcagtctggc tctcagctcc gggctctcag
     3661 ctctcagctt caattcctcc agccatgaat ctctctctct actcggccgc caccggcatg
     3721 gaggcgcaac agctcaatct gaacaccatc gcgaacaacc tcgcgaacgt gaacacgccc
     3781 ggcttcaagc gcagcaagat cgagtttcag gatcttcttt accagaaacc cacccgcgct
     3841 tccggcaccg actcgggcgg cggcaacatc gtcccgaccg gcatcgaggt cggcaacggt
     3901 tcgcgcgtcg cctcgacgtc gaaggtcttc acgcaaggcc agctcacgtc caccggcgag
     3961 aagctcgaca tcgcgattca gggcgacggc tttttcgaag tgcagcgtcc cgacggcacg
     4021 atcggttaca cgcgcgacgg atcgttcaag ctcaacgcgc aaggccaggt cgtcaccatc
     4081 gacggtcttc ccgtgctcag cggatttcag ccgattcctc ccggcgccag cggcgtcagc
     4141 atcgcggaaa acggacaggt caccgtgcag agttcgaccg gctcgaccac gttccgcctc
     4201 agcctcacgc gtttcgccaa tccttccggt ctgcgcagca tgggcggcaa tctctacgaa
     4261 gaaaccgccg cgagcggcac gcccgaaatc ggccaacccg gcgaactcgg cttcgggagc
     4321 ctcatgcagg gctacatcga atcttccaac gtcaacatcg tcgaagaaat ggtgaacctc
     4381 atcgtcgcgc agcgcgccta cgagattaac tccaagtcga ttcagacttc cgacgagatg
     4441 cttcaaaaca tcgcgcagat gaaacgctga gcccatgcgc ttcctcgctc atctcatccc
     4501 gcaatcgcac cgcgagctca cgcgaaaccg tttcgttcgc gtgctcttcc tcgcgtccgc
     4561 cgtgctcgtc gcgccgctcg cgttcgccgc cgcgccggcc gccgccaagg ccgtcgaagc
     4621 caccgcgacg ccgctg
//
LOCUS       DASPLT010000523         1271 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_272101, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000523 DASPLT010000000
VERSION     DASPLT010000523.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1271)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1271)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1271
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_272101"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..522)
                     /locus_tag="VEA63_05395"
     CDS             complement(<1..522)
                     /locus_tag="VEA63_05395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor"
                     /protein_id="HYD83460.1"
                     /translation="MSVSDTDFPADATPPAAPPASASTSQLVEHFFRHETGRLHGALI
                     RLLGVHNLSLAEDVAQEAMLRALHSWAMGGVPANPSAWITRVAMNIARDMLRHQRMSG
                     GKESAIVTYLEQTRSAPPAAADSDMEIRDDALRLMFVCCHPSIAADAQVVLALKVLCG
                     FSTSEIARAFLSSD"
     gene            complement(528..947)
                     /locus_tag="VEA63_05400"
     CDS             complement(528..947)
                     /locus_tag="VEA63_05400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007364094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HYD83461.1"
                     /translation="MPTQPAPSPYMLLFRNSGPENHQHLSPEQRQQLVTRWNAWFDEL
                     TTQGKAIEGQPLEMETRIVSGRNGQRVVDGPFAEAKEAIGGYVMLMVNGLDEATAIAQ
                     RHPGLEYGLVIEVRKLTEDCHLGVNTRGRAANIAASA"
BASE COUNT          234 a          379 c          434 g          224 t
ORIGIN      
        1 gtcgctggaa agaaaggcgc gcgcgatctc gctcgtgctg aaaccgcaga gaactttcaa
       61 ggcgagcacg acttgcgcgt cggcggcgat cgagggatgg cagcagacga acatcaggcg
      121 cagcgcgtcg tcgcgaattt ccatgtcgct gtcggcggcg gccggcggcg ccgagcgcgt
      181 ttgctcgaga taagtgacga tcgccgattc ctttccgccg gacatgcgct ggtggcggag
      241 catgtcgcga gcgatgttca tcgcgacgcg tgtgatccag gccgagggat tcgccggcac
      301 gccgcccatc gcccacgaat gcagtgcccg caacatcgct tcctgcgcga cgtcttcggc
      361 gagggaaagg ttatgcacgc ccagcaggcg aatcaacgca ccgtggagcc gacccgtttc
      421 gtgacggaaa aagtgctcca ccaactgcga cgtgctggcc gacgcgggcg gtgcagccgg
      481 cggggtggca tcagccggaa aatcggtgtc cgataccgac atggcgttta cgccgaagcg
      541 gcgatgttgg cggcccggcc gcgcgtattc actccgaggt gacaatcctc ggtgagtttt
      601 cgcacctcga tcacgagccc atattcgagg ccgggatggc gttgagcgat cgccgtggct
      661 tcgtcgagcc cgttgaccat gagcatgacg taaccaccga tggcttcctt ggcttcggcg
      721 aacgggccgt cgaccacgcg ctggccattg cgtccggaaa cgatgcgagt ctccatctcc
      781 aacggttgtc cctcgatcgc cttgccctgg gtcgtgagct cgtcgaacca ggcattccag
      841 cgcgtgacga gctgctggcg ttgctcaggc gaaaggtgct gatggttttc gggaccgctg
      901 ttgcggaaga gcaacatgta agggctggga gcgggttggg ttggcatagg ggaaggagga
      961 ttgagagttc gagaggaagt cgccgtcaca agcttcaacg aacgaacttc gcatcgtcgg
     1021 acaaggtagt gcaacttttc cggcgacgtt ccacgccgcg ccgagcggca gacctccgtg
     1081 ccggccggcc tctgttcaga acggtaggtg gcgcgctcgt tgcgcgcgcg cgcaacctac
     1141 cgggcgagct cggagccgag cggagcgttc gtcgttgcgc ggaaggcttc gcttggcatg
     1201 ttcggccgcg ccgccgacgc ggtggaaagc ggaccgctga aaacaaacgc atggagtcaa
     1261 acgaccactc g
//
LOCUS       DASPLT010000524         1344 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_149552, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000524 DASPLT010000000
VERSION     DASPLT010000524.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1344)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1344)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1344
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_149552"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..227
                     /locus_tag="VEA63_05405"
     CDS             <1..227
                     /locus_tag="VEA63_05405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD83462.1"
                     /translation="KSARFSPAEAPSLPSPLSVEPDPVSAAAGARRGCFIVAAIVALL
                     GAIALTTVYFVRYRDRPLIFVEKPADASRP"
     gene            344..808
                     /locus_tag="VEA63_05410"
     CDS             344..808
                     /locus_tag="VEA63_05410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD83463.1"
                     /translation="MNSAALPTDRINDSHWNDVRRIYRRVCVLRATGRPAEALDLEID
                     EFAKALASARAASGGDEETEAAVLAQENERVTNATVLAELVAPLLAERLHAQMPVPAV
                     SASVMPARKKSSSPDSSPAPAKTPPLTPPSIADLLDGMLAQSPALTAAGAHP"
     gene            871..>1344
                     /locus_tag="VEA63_05415"
     CDS             871..>1344
                     /locus_tag="VEA63_05415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD83464.1"
                     /translation="MSTAVEKPLPAAAVPANAPNVTVSRSASTPSKTKTVLVGFDFGT
                     NKSCVLAGSAANTDIAVSKVVPTVVGYVKDGIVDGIVAGNRSVLFGDDALQNMLHVRL
                     VQPMTHGVVTEPEAARDFLKHIRSLADPSGSAEIRAVVGVPANAAEQEREDIRRTA"
BASE COUNT          228 a          512 c          347 g          257 t
ORIGIN      
        1 cgaaatccgc ccgcttctcc cctgccgaag cgccgtcatt accctcaccg ctttcggtcg
       61 agccggatcc agtgagcgcc gccgcgggcg cacgtcgcgg gtgtttcatc gtcgccgcca
      121 ttgtcgccct gctcggtgcg atcgcgctca cgacggttta cttcgtccgc tatcgcgacc
      181 ggccgcttat ttttgtcgaa aaacccgccg acgcgtcgcg cccttgaggc gtcttcacaa
      241 gcgttcaaaa taacctcttc cgccgcggct tcgatcgcgc cgcatttcac ttttttcgaa
      301 tccacggaaa ccatcgcaca gcctgccggt ctagcgcctg atcttgaaca gcgcagcgct
      361 gcccaccgat cgcatcaacg actcccattg gaacgatgtc cgccggattt atcggcgcgt
      421 ctgtgtgttg cgcgcgactg gccgaccggc cgaggcgctt gatttggaaa tcgacgagtt
      481 cgcgaaagcc ttggccagcg cccgcgctgc ttccggtggc gacgaggaaa ccgaggccgc
      541 cgtgcttgcc caggaaaacg aacgcgtgac caacgccacc gtcctcgccg aactcgtcgc
      601 tccattgctc gcggagcgtt tgcacgcaca gatgcccgtg cctgcggtct cagcttccgt
      661 gatgcccgcc cggaaaaaat cctcttctcc cgattcttct cccgcgccgg ccaaaacgcc
      721 cccgctcacg ccgcccagca tcgccgacct tctcgatggc atgctcgcgc aatcacctgc
      781 gctcaccgcc gccggcgcgc atccctgaat ttcagtcctc ccgcttcgac gttcgctccc
      841 gatacatcac aaccctcgtt cctcctcatt atgtccactg ccgtcgaaaa acctcttcct
      901 gccgccgcgg ttcccgccaa cgcgccgaat gtgaccgtct cgcgctccgc gagcacgcct
      961 tcgaaaacca agacggtgct cgtcggcttc gacttcggca ccaacaagtc ctgcgtcctc
     1021 gccggctccg ccgccaacac cgacatcgcc gtcagcaaag ttgtccccac cgtcgtcggc
     1081 tacgtaaagg acggcatcgt cgatggcatc gtcgccggta atcgctcggt tctctttggc
     1141 gacgatgcgc tgcaaaacat gctccacgtc cgcctcgtgc aaccgatgac gcacggcgtc
     1201 gtcaccgagc cggaagccgc ccgcgatttc ctaaaacaca tccgctcgct cgccgatcct
     1261 tccggcagcg ccgagatccg cgccgttgtc ggcgtgccgg ccaatgccgc cgaacaggaa
     1321 cgcgaagaca tccgccgcac cgcg
//
LOCUS       DASPLT010000525         1285 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_88558, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000525 DASPLT010000000
VERSION     DASPLT010000525.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1285)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1285)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1285
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_88558"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..537
                     /locus_tag="VEA63_05420"
     CDS             <1..537
                     /locus_tag="VEA63_05420"
                     /inference="COORDINATES: protein motif:HMM:NF013257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HYD83465.1"
                     /translation="LEQRQLSRGDLVLIQAGNKKTMKGGDRRRASNVDYWPETNWAEV
                     IAGVLQTLPSSRVILCGSPAERPLAEDIVSHLAPSARDRVAIATDDLPIPRLFALQER
                     AHSLISVNTGPAHAAAAMGCPLLVMFTRHLHRAADLYAPVATTAFVKVIQPDSTAPDA
                     GLDQITPAAVLAGWRNLI"
     gene            584..>1285
                     /locus_tag="VEA63_05425"
     CDS             584..>1285
                     /locus_tag="VEA63_05425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HYD83466.1"
                     /translation="MPLLTVTDLRTYFHTRSGVYRAVDGVSFSIKRGETLGIVGESGS
                     GKSVTCYSLLGLVPQPPARIESGTALFDDVDLLHCSPAQARAIRGKRIAMIFQDPMTS
                     LNPYLRVSEQLIEPLLIHENISRRDALARARTMLDAVGINDAAKRIHFYPHEFSGGMR
                     QRVMIAMALITKPELLIADEPTTALDVTVQAQILELIKSMQRELGMAVIFVTHDLGVV
                     SGLCDRVQVMYAGRIV"
BASE COUNT          228 a          502 c          331 g          224 t
ORIGIN      
        1 ctcgaacaac gacagctctc acgcggagac ctcgttctta ttcaagccgg caacaagaaa
       61 accatgaaag gcggcgaccg tcgacgcgcc agcaacgtcg attattggcc cgagacaaac
      121 tgggccgagg tcatcgctgg cgtccttcaa acactgccgt cttcgcgcgt cattctctgc
      181 ggctcgcccg ccgaacgccc gctcgccgag gacatcgttt cccacctcgc tccttccgcg
      241 cgcgaccgcg tcgccatcgc caccgacgac cttccgatcc cgcgcctgtt cgcgcttcag
      301 gagcgcgcgc acagcctcat cagcgtgaac accggccccg cccacgccgc cgccgccatg
      361 ggctgtccgc tgctcgtgat gttcacccgc cacctgcacc gcgcggccga tctttacgcg
      421 cccgtcgcca ccaccgcctt cgtgaaagtc atccagcccg actcgaccgc acccgacgcc
      481 ggcctcgacc aaatcacgcc cgccgccgtg ctcgcgggat ggcgcaacct catttaagtt
      541 tgcggcgcct caccgccacc gcaccgtcgt cggcagctac gcgatgccac tgctgacggt
      601 caccgatctt cgaacttact tccacacgcg ctccggtgtt taccgcgcgg tcgatggcgt
      661 cagtttctcg atcaaacgcg gcgaaacact cggcatcgtc ggcgaatccg gctccggcaa
      721 atccgtcacc tgctactcgc tcctcggcct cgtcccccaa ccgcccgcgc gcatcgaaag
      781 cggcaccgcg ctcttcgacg acgtggacct gctccactgc tcgcccgccc aggcacgcgc
      841 catccgcggc aagcgcatcg cgatgatctt tcaggatccg atgacgtcgc tgaatcccta
      901 cctgcgcgtt tccgaacaac tcatcgagcc actgctcatc cacgaaaaca tttcgcgccg
      961 cgacgccctc gcccgcgcgc gcacgatgct cgacgccgtg ggcatcaacg atgccgccaa
     1021 acggatccac ttttacccgc acgaattttc cggcggcatg cgacagcggg tgatgatcgc
     1081 gatggcgctc atcacgaaac cggaactgct catcgccgac gagcccacca ccgcgctcga
     1141 cgtcaccgtg caggcgcaaa ttctcgagct catcaaatcc atgcagcgcg agctcggcat
     1201 ggccgtgatt ttcgtgacgc acgatctcgg cgtcgtctcc ggcctctgtg atcgcgtcca
     1261 agtcatgtat gccggccgca tcgtc
//
LOCUS       DASPLT010000526         1377 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_149564, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000526 DASPLT010000000
VERSION     DASPLT010000526.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1377)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1377)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1377
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_149564"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            23..>1377
                     /gene="recN"
                     /locus_tag="VEA63_05430"
     CDS             23..>1377
                     /gene="recN"
                     /locus_tag="VEA63_05430"
                     /inference="COORDINATES: protein motif:HMM:TIGR00634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HYD83467.1"
                     /translation="MEKLRVRFHAGLNVLTGETGSGKSIVVDALGLLFGGRASNEMLR
                     SGADRARISGIFEAPKAAIRTLSDAGIETEDGEVLIEREILAGGKSRAFIANRPVTAA
                     LLRELSPHFGDIHGQHDQQQLFSPDAQLDIVDSYGKAAPLLERVAGVYREWRACSAEL
                     AELERTEQEKLRLLDLWTFQRNEIESARLKPGEDAELENERRVLQNVGKLMDAANTAY
                     LALYDANESAYAQLRLALKRIEELCRIDESLTGVAETLKPAQIAVEDASATLRDYLGK
                     LEADPARLDEIENRMASLEKLKRKYGPDSESILAFLEEVQRDIAAAESSAERRAEIET
                     RRKELAAEYEAAAAKLTEARRSAASSLAKKVQSELKSLAMERTVFEVRLTPAAWSAHG
                     ADAAAFFVSANVGEEPRPLEKVASGGELSRIALAIKTCVTAAPKGRTLVFDEVDAGIG
                     GT"
BASE COUNT          277 a          393 c          479 g          228 t
ORIGIN      
        1 acacgtggaa aactacgcgg ttgtggagaa gctgcgcgtg cgcttccatg ccggcctgaa
       61 tgttctgacg ggcgagacgg gcagcggcaa atccatcgtg gtggacgctc tcgggctgct
      121 gttcggcggg cgcgcttcga acgagatgct gcggtcgggc gctgaccgcg ctcgcatctc
      181 cggtatcttc gaagcgccta aggccgcgat tcggaccttg agcgacgccg gcatcgaaac
      241 ggaagacggc gaggtgctga tcgaacggga gatcctggcc ggcgggaagt cgcgcgcgtt
      301 catagcgaac cgtccggtca ctgccgcctt actgcgtgag ttgtcgccgc atttcggcga
      361 tattcacggg cagcacgacc aacagcagct cttcagcccc gatgcgcagc tggatattgt
      421 cgattcatat gggaaagccg ctccgctgct agagcgggtc gccggagtgt accgcgagtg
      481 gcgcgcgtgc tccgcggagc tcgccgagct ggagcggacc gaacaggaga aactacgcct
      541 gctggatttg tggaccttcc agcggaacga gatcgagtcg gcacgcctga agccgggcga
      601 ggatgccgag ctggaaaatg agcggcgggt actccagaat gtcggaaagc tcatggatgc
      661 cgccaatacg gcgtatctgg cgctctacga cgcgaacgag tcagcctatg ctcagctccg
      721 gctggcgctg aagcggattg aggagctgtg ccggatagac gaaagcctga cgggcgtagc
      781 ggagactctg aagccggcgc agatcgccgt ggaggacgcc agcgcgacac ttcgggatta
      841 tctcggaaag ctggaagccg atcccgccag gctagatgaa atcgagaacc gtatggcctc
      901 gcttgaaaaa ctgaagcgaa aatatggccc cgattcggag tcgattcttg cctttctgga
      961 agaagtgcag agagatatag cggcggcaga gtcgtcggcg gagcgtcgtg cggaaattga
     1021 gacacgccgt aaggaattgg ccgccgagta cgaggcggcg gccgcgaagc taacggaggc
     1081 gaggcgctcg gccgcatcct cgctcgcgaa gaaagtgcag agcgaactga agtcgctcgc
     1141 catggagcgg acagttttcg aggtcaggct tacgcccgcc gcgtggtcgg ctcacggcgc
     1201 cgatgccgcc gcattcttcg tatcggcgaa cgtcggagag gagccccggc ctctcgagaa
     1261 ggtggcatcc ggcggcgagt tgtcgcgcat cgccctcgcg atcaagacgt gcgtgaccgc
     1321 cgcccccaaa ggcaggacgc tggtgttcga cgaagtcgac gcgggcatcg gcggcac
//
LOCUS       DASPLT010000527         2113 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_88579, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000527 DASPLT010000000
VERSION     DASPLT010000527.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 2113)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2113)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2113
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_88579"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..495
                     /locus_tag="VEA63_05435"
     CDS             <1..495
                     /locus_tag="VEA63_05435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HYD83468.1"
                     /translation="NINVTGSYLVGDEAAKTWREQGLRGNLVLTTSANAVVSKKGSLA
                     YDTSKAAANHLVRELAVELAPLVRVNGVAPATVVQGSAMFPRDRVIGSLAKYNIPYTD
                     DEATDSLVRKLAQFYADRTLTKSPITPADQAEAYFLLVTQRLSKTTGQVITVDGGLHE
                     AFLR"
     gene            669..>2113
                     /locus_tag="VEA63_05440"
     CDS             669..>2113
                     /locus_tag="VEA63_05440"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007361205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase family protein"
                     /protein_id="HYD83469.1"
                     /translation="MTSVSKNKRSIRSQPAQSSTPLHCAAVDLGATSGRVIVGTWAKN
                     KLTLTEVHRFPNAFASLDGHDYWNLPGLWTEIQTGLRRAAEQFKPIASIGVDTWGVDY
                     ALTDDAGRLVYPVHAYRDARTQPGLQKLANTRAALARLYAATGIPNVFYNSSLQLAET
                     VAAHPAIEDLATRCLFLPDYFNFLLSGRMENEFTIASTSQLIDVHSTDWSRPALDYFR
                     IPSQWFSKPILAGTKLGKLRRELRTGFSSFNDTQIIAVPGHDTASAYDAMPADPSGTD
                     LFLSSGTWSLVGFESDTPVLGAEALAARISNERTGDGRYRPLTNVIGLWLLECTLKDF
                     SSRPRNDTEWAALIKAAEKLRPTRGSARERRDSELNELLDVTDPAFANPPSMRAAIDA
                     HLKRRKLPLPKNLPAYVRLICDSLGRGHADAMRAFERLANRKFKRILMVGGGSKNRLL
                     CQATADAANVPVISFALEGTAVGNIASQLIAL"
BASE COUNT          395 a          830 c          501 g          387 t
ORIGIN      
        1 aacatcaacg tcaccggcag ctatctcgtc ggcgacgaag ccgcgaagac ctggcgcgaa
       61 cagggcctcc gcggcaacct ggtcctcacg acgtccgcca acgccgttgt atccaaaaaa
      121 ggatcactcg cctacgacac ctcgaaagcg gccgcgaacc acctcgtgcg cgagctcgcc
      181 gtcgagctcg ctccgctcgt tcgcgtcaac ggtgtcgcgc ccgcgaccgt cgtccaaggc
      241 tccgcgatgt ttccccgcga ccgcgtcatc ggctcgctcg cgaaatacaa catcccttac
      301 accgacgacg aagcgaccga ctccctcgtt cgcaaactcg cgcagttcta cgccgatcgc
      361 accctcacga aatcgccgat cacgcccgcc gatcaagccg aggcgtattt cctcctcgtg
      421 acgcagcgcc tcagcaaaac gaccggccag gtcatcaccg tcgacggcgg cctccacgag
      481 gccttcctcc gctaaatatt cgtagcccgc tgcgtgagca gcgggctact tccctctctt
      541 tctctaaaaa ttacacagag aacacagagt tcgagccgga gttcactgag gtttttccct
      601 gctctcccgc ccccgcgccc atcatcgccc acttctaact tcgcctctgt ggcctcagtg
      661 cttcctccgt gacctctgtg tcaaaaaaca aacgctccat ccgctcgcaa cccgcgcaat
      721 catcgacgcc gcttcactgc gccgccgtcg atcttggcgc cacgagcggc cgtgtgattg
      781 tcggcacgtg ggccaaaaac aaactcacgc tcaccgaagt ccaccgtttc cccaacgcgt
      841 tcgcttccct cgacggtcac gactactgga atcttcccgg cctctggacc gaaatccaaa
      901 ccggtctccg ccgcgccgcc gaacagttca aacccatcgc ctccatcggc gtcgatacct
      961 ggggcgtcga ctacgcgctg accgatgacg ccggccgcct cgtgtatcca gttcacgctt
     1021 acagagacgc ccgcacgcag cccggcctgc aaaaactcgc caacacccgc gccgccctcg
     1081 ctcgtctcta cgctgccacc ggaatcccga acgtcttcta caactccagc ctccaactcg
     1141 ccgagaccgt cgccgctcac cctgcgatcg aagacctcgc cacgcgctgc ctttttctcc
     1201 ccgactactt caatttcctt ctcagcggcc ggatggaaaa cgagttcacg atcgcgagca
     1261 cctcgcagct catcgacgtt cactccaccg actggtcccg tccggcgctc gattacttcc
     1321 gcattccgtc gcagtggttt tcgaagccga tcctcgccgg caccaagctc ggtaaactcc
     1381 gccgtgaact ccgcaccggt ttctcttcgt ttaacgacac ccaaattatc gccgtccccg
     1441 gccacgacac cgcgtccgcc tacgacgcga tgcccgccga cccatccggc acggatcttt
     1501 ttctcagctc cggcacctgg tcgctcgttg gtttcgaaag cgacacccct gtcctcggcg
     1561 ccgaagcgct tgccgcgcgc atctccaacg aacgcaccgg cgacggccgc taccgcccgc
     1621 tcacgaatgt catcggtctc tggctgctcg agtgcacgtt aaaggatttc tcctcgcgcc
     1681 cccgcaacga caccgagtgg gccgcgctca tcaaagccgc ggaaaaactt cgcccgaccc
     1741 gtggctctgc gcgtgagcgc agggattctg agctcaacga gctcctcgac gtcaccgatc
     1801 ccgctttcgc gaacccgccc tctatgcgcg ccgcgatcga cgcccatctc aagcgccgca
     1861 agctcccgct tccgaaaaac cttcccgctt acgtccgctt gatctgcgac tcgctcggcc
     1921 gcggccacgc tgacgcgatg cgcgccttcg aacgcctcgc gaaccgaaaa ttcaaacgta
     1981 ttctgatggt cggcggtggc tcgaaaaacc gcctcctctg ccaagccacg gccgacgccg
     2041 ctaacgttcc cgtcatctcc ttcgccctcg aaggcaccgc cgtcggcaac atcgccagcc
     2101 agctcatcgc cct
//
LOCUS       DASPLT010000528         2982 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_107851, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000528 DASPLT010000000
VERSION     DASPLT010000528.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 2982)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2982)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2982
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_107851"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..300
                     /locus_tag="VEA63_05445"
     CDS             <1..300
                     /locus_tag="VEA63_05445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007362529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HYD83470.1"
                     /translation="GEVKAALAEINVEGMTVTEVKGFGRQKGHTEIYRGSEYTVDFLP
                     KVKIEIATTDALASKAVEAIVKSAKTGKIGDGKVFVLPIEDVIRIRTDERGESAI"
     gene            618..956
                     /locus_tag="VEA63_05450"
     CDS             618..956
                     /locus_tag="VEA63_05450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007362529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HYD83471.1"
                     /translation="MKLIIAVIKPFKLEEVKAALAAISVEGMTVTEVKGFGRQKGHTE
                     IYRGSEYTVDFLPKVKIEIVTSDAIAPKAVEAIVKAAKTGKIGDGKVFVVPLDDAIRI
                     RTSERGDSAI"
     gene            1213..>2982
                     /locus_tag="VEA63_05455"
     CDS             1213..>2982
                     /locus_tag="VEA63_05455"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HYD83472.1"
                     /translation="MKISPLAAGCLLAVPFLATAAELPTTVAAFQSEATRHHVALVLP
                     TYPETVEKVRAMSDRVLNEADAALARLAALAPDALTFENTFAAYDAIAGDVSSFAGQI
                     ETVAETHPTQEMRDTAREMSVRIQEWGIALDYREDIYRALKAFADTNPKLDAQEQRLM
                     DFELRNYRRAGLALPAEQRAAVEQLRKQLSALEQEFSVNINQARAPLDFSADELAGVP
                     QTLLGSPDVRQPDGRFRVMAHITWHYMAVMDNATKAATRQRLYLARNTLAKETNVAVL
                     ARLVALRADIADRLGYATWADYRTEVRMAKTGATALKFEEDLVAGLQPKFAAELATLR
                     ELKRAEAGDAQAEIEAWDPSYYMNLLKKQRYAVDEEQLRVFFAYEPTLRGMFDIYQRI
                     FGLTFTEVTPPWVWTPGVQLFVVTDASTDAPIGSFYLDMFPREGKYNHFACFPQMSGR
                     VLPDGRYELPVATLVCNFPPPAGDAPSLLSHDNVETLFHEFGHVMHMLLGRARFQGQT
                     SGAVPRDFVEAPSQMLESWVWDKKVLDTFAADYRDRAKKIPAETIEALTQARVATDGY
                     FNRRQLSLGLVDLSLHTLSFTEAN"
BASE COUNT          578 a          868 c          994 g          542 t
ORIGIN      
        1 ggggaagtga aggccgcgct ggccgagatc aacgtcgagg gtatgaccgt gacggaagtc
       61 aaaggcttcg gccgccagaa aggtcacacc gaaatctatc gcggaagcga atacacggtc
      121 gatttcctgc cgaaggttaa aattgaaatc gcgaccaccg acgcgctcgc cagtaaagcg
      181 gtggaagcga tcgtgaaatc ggcgaagacc gggaaaatcg gcgacggcaa agtgttcgtt
      241 ttgccgatcg aggacgtcat tcgcatccgc acggacgaac gcggcgagtc ggcgatctaa
      301 gcacgcagac gctcgccaat aaaacagccg gctcttcgga gccggctttt tttgggggtc
      361 aggttgcgag cgggtttcag gttttgacgg tgggcggtgc gggctcgttg ccgtgtccgg
      421 cgtccgccgc attgtgtgat ctcgcgctgg cgaagcctcg gcaggcaggc gcggaggcct
      481 gcgctacggg gagacatcaa gtgggcgccg attgcggcgc cggcgaagca tcgtgcttgc
      541 gcgaaacaac taaagcgcct cagttgggaa gcgttcaccg cgcggttgcg gatcgcgcac
      601 atcattagcc cattctcatg aaactgatca ttgccgtcat caagccgttc aaactcgagg
      661 aagtgaaagc cgccttggct gcgattagtg tcgaaggcat gaccgtcacc gaggtcaagg
      721 gcttcggccg ccaaaagggg cacacggaga tttatcgcgg cagcgaatac accgtcgact
      781 tcctgccgaa ggtgaagatc gagattgtga cgtcggacgc catcgcgccg aaagcggtcg
      841 aggcgatcgt gaaagcggca aagacgggaa aaatcggtga cggcaaagtg tttgtagtgc
      901 cgttggacga cgcgattcgc atccgcacca gcgagcgcgg cgatagcgcg atttgagcga
      961 ggagcacgcg cgaaacgcgt atttgtagcg gcaagcctcc gcgcctgcct gctcgcattt
     1021 tcaagtaggt ggcgcgcttg cgcgccacag cgtgcgtgag cgtgtagcct tacggcagca
     1081 ggcaggcgcg gaagcctgcc gctacgctga atcctctccc gcggagttcg tggcggcggc
     1141 gcgctcggct gaaagagatt tgcgtgatct cgcgtggtgc gttttctgca tcgtttcgcc
     1201 cgcgatcttc tcatgaaaat ttcccctctc gccgccggtt gtttgctggc ggttcctttc
     1261 ctggcgacgg ccgccgaact cccgacaacg gtcgccgcgt ttcaatcgga ggcgactcgg
     1321 caccatgtcg ccctcgtcct gcccacttat ccggaaacgg tcgagaaagt gcgcgccatg
     1381 agcgatcgtg tgctgaacga agcagatgca gcgctcgcga gactcgcggc gctggctcct
     1441 gacgcgctga cgttcgagaa cacgttcgcc gcctacgatg cgatcgcggg cgatgtcagc
     1501 agttttgccg gacaaatcga gaccgtggcc gagactcatc cgacgcagga aatgcgcgac
     1561 acggcccgcg agatgtccgt gagaatccag gagtggggga tcgccctcga ttatcgcgaa
     1621 gacatttatc gcgcgctgaa agcgttcgcc gacacgaacc cgaagctcga tgcgcaggag
     1681 cagcggctca tggatttcga gttgcgcaac tatcgccgtg cagggctcgc gctgccggcc
     1741 gagcaacgcg ccgccgtcga gcagttgcgg aagcagcttt cggccctcga gcaggagttt
     1801 tccgtgaaca tcaaccaggc gcgcgcaccg ctcgacttct cggccgatga actcgcgggc
     1861 gtgccgcaga ccctgctcgg cagccccgac gtgcgtcaac ccgacggccg cttccgcgtg
     1921 atggcgcaca tcacgtggca ctacatggcg gtcatggaca acgccacgaa ggcggcgacc
     1981 cgccagcgcc tctatctcgc gcgcaacaca ttggcgaagg agacgaacgt cgctgtgctc
     2041 gcgcggcttg ttgcactgcg cgccgacatc gccgaccggc tcggctacgc gacgtgggcg
     2101 gactatcgga ccgaagtgcg gatggcgaag accggcgcaa cggcgttgaa gttcgaggag
     2161 gacctggtgg cgggactgca gccgaagttc gcagcggagc tcgcgacgct gcgggagttg
     2221 aagcgggccg aggcgggcga tgcgcaggcg gagatcgagg cctgggaccc gagctattac
     2281 atgaacctgc tcaagaagca gcgctacgcg gtggatgagg agcaactccg ggtgttcttc
     2341 gcctatgagc cgacgttgcg gggaatgttc gacatttatc aacgcatctt cgggctgacg
     2401 ttcaccgaag tgacgccgcc gtgggtgtgg actccggggg tgcagttgtt cgtcgtcacg
     2461 gacgcgtcca cggacgcgcc gatcggtagt ttttatttgg atatgttccc gcgcgaaggg
     2521 aaatacaacc acttcgcctg cttcccgcag atgtcaggtc gcgtgctgcc ggacgggcgt
     2581 tacgaactgc cggtggccac actggtgtgc aatttcccgc caccagcggg agacgcgccg
     2641 tcattgctgt cgcatgacaa cgtcgagacg ttgtttcacg aatttggcca cgtgatgcac
     2701 atgctgctcg gacgcgcgcg ttttcagggg cagacctcgg gcgcggtgcc gcgggatttc
     2761 gtggaggcgc cgtcgcagat gctggagagc tgggtgtggg acaagaaggt gctcgacacc
     2821 tttgccgcgg attatcgcga ccgggcgaaa aaaattccgg cggagacgat cgaggcgctg
     2881 acgcaggcgc gggtcgcgac ggacggatat ttcaaccggc ggcagctttc gctcgggctc
     2941 gtggatctct cgttgcacac cctgtcattt accgaggcca ac
//
LOCUS       DASPLT010000529         1278 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_88634, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000529 DASPLT010000000
VERSION     DASPLT010000529.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1278)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1278)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1278
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_88634"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(23..925)
                     /gene="rarD"
                     /locus_tag="VEA63_05460"
     CDS             complement(23..925)
                     /gene="rarD"
                     /locus_tag="VEA63_05460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HYD83473.1"
                     /translation="MLAKPNPSAVRGAFAAAGAYLAWGLFPLYWKQLSSVNAVEVIAH
                     RHLWSLGFVMLAMLVGGTMSELKAALVSARALAVHTASGVLLTINWLVFIWGVNAGYV
                     LEASLGYFLVPLVNVALGRFVLHERLRWLQWTAIALAAAGVVLLLIRVGRLPWIALSL
                     AGTFGAYGLLRKKSPLGALTGLGLETLLVAPLAVGFLTWRQLAGTGALGQASVGVHAL
                     LIGAGVVTAVPLVSFAFGARRIRFTTLGLLQYIAPTAQFAIGVWVYREPFSSERAAAF
                     GFIWAGLVLYTIDNLWAQRRLKVS"
     gene            complement(1018..>1278)
                     /locus_tag="VEA63_05465"
     CDS             complement(1018..>1278)
                     /locus_tag="VEA63_05465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD83474.1"
                     /translation="KSSLLLCLCAVVLASAPGCNVFRKSKKPKANPNIAAEVETNFRE
                     RWMERRVAELTAQGTDPAAAREQAAIDFREKFPHLKTVSGGK"
BASE COUNT          274 a          475 c          349 g          180 t
ORIGIN      
        1 ttcgaaatgg agctcatctc cgtcacgaaa ccttgagccg gcgctgcgcc cacaggttgt
       61 cgatcgtata aagcacgagc ccggcccaaa tgaagccgaa ggccgccgcc cgctccgacg
      121 aaaacggctc gcgatagacc cacacgccga tcgcgaactg cgccgtcggg gcgatgtatt
      181 gcaacagccc aagtgtcgtg aaccgaatcc gccgcgcacc aaaagcgaac gacaccagcg
      241 gcaccgcggt gactaccccg gcgccgatca gcaatgcatg cacgcccaca ctcgcctgcc
      301 ccagcgcccc cgtgcccgcc agctgccgcc acgtgagaaa ccccacggcg agcggagcaa
      361 ccaacagcgt ctccagccca agtccggtca acgcacccaa cggcgatttt tttcgcaaca
      421 acccgtaggc gccgaaggtg cccgcgagcg agagcgcgat ccacggcagc cggcccacgc
      481 gtatcagcag aagcacgaca ccggccgccg ccagcgcgat tgcggtccat tgcagccagc
      541 gcagccgttc gtggagcaca aaccggccga gcgccacgtt caccaacggc acaagaaaat
      601 aacccagact cgcctccagc acataccccg cgttcacgcc ccagatgaac accagccagt
      661 tgatcgtgag cagcaccccg ctggccgtat ggactgcgag cgctcgggcc gagaccagcg
      721 cggccttgag ctcgctcatc gtgcctccga ctagcatcgc cagcatcacg aaacccagcg
      781 accagaggtg acggtgcgca atcacctcca ccgcgttcac ggaggacaac tgtttccaat
      841 aaagcggaaa caatccccac gccagatacg cccccgcagc ggcgaatgcg cctcgcaccg
      901 cggatggatt cggtttcgcc agcatgcgcg cgacggtcgc gggtccacgc ctcgggtcaa
      961 cgccccaacc gctgtgccgc tcgttccggc gaacgggtgc gcaggtcgcc cggaaggtta
     1021 tttcccgcca cttaccgtct tcagatgcgg aaatttttcg cgaaaatcga tcgccgcctg
     1081 ctcgcgcgcc gccgctggat cggttccctg cgcagtcagc tccgccacgc gccgctccat
     1141 ccaacgctcc cggaaattcg tctccacctc cgctgcgatg ttcggattcg ccttcggttt
     1201 cttcgacttg cgaaacacgt tgcagccagg agccgacgcg agcacgaccg cgcacagaca
     1261 aagcagaagc gaagactt
//
LOCUS       DASPLT010000530         3857 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_88695, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000530 DASPLT010000000
VERSION     DASPLT010000530.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 3857)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3857)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3857
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_88695"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2521
                     /locus_tag="VEA63_05470"
     CDS             <1..2521
                     /locus_tag="VEA63_05470"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070569 - uridylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase"
                     /protein_id="HYD83475.1"
                     /translation="LAQLLGAIFGPGRGFELVSNVNRIPKGSRLAVSTNLLGALIGVC
                     MRATSQIRSLTGPLEESERRIVAARAILGEWLGGSGGGWQDSGGLWPGIKLIEGAPAQ
                     PGDPEHGISRGTLLPRHTLLGADRISPEARQKLQDSLVLVHGGMAQNVGPILEMATEK
                     YLLRSEAEWSARLKTVATLDDILALLAAGDIKQLGAALTENFFGPLQAIIPWVSNLYT
                     ERLIERTRAAFGEDFWGFWMLGGMSGGGMGFIFAPHRKREGQEKLREIMLTTKRELES
                     SLPFAMDPVVYDFAINEHGTVAALLTGPDALLPADFYRATIPAYLRRSARELTNRERG
                     DLQQFTRAARTLPEFSAALPTLLDQLLPSGESRDGGSQRLAELLAQNGFDRAQHEQIR
                     ADLRSGRIGLAMNRLPANTLIEDVPPTALFNYAEVGTPLRGVRPDPASPNPKPKTQNS
                     ELEEFAAGEAALKRGELAVVTYAAGVGSRWTQGAGVVKGLHPFAKLAGRHRSFIETHL
                     ARTRHTAREFGTTIPHIFTTSHLTHAPIEHWLRMHAADALDRDVFLSPGRSIGLRLVP
                     AVRDLQFAWEETAQQKLDEQKEKMRESIRAALTNWARTVGEGADYTDNLPEQCLHPVG
                     HWFEIPNLLKNGTLARLLSAQPQLRTLVVHNIDTLGASPDPTLLGWFQSTGATLGWEV
                     IPKRIEDHGGGLARVDGRLRIVEGLALPREDDEFNLTYYNSNTCWIDIDRLLTVFGLT
                     RKDLADTEKVAAAVRTLAARLPTYVTLKDVKKRWGHGQEDVYPVAQFEKLWGDMTALP
                     ECTSVFAAVPRARGQQLKDQAQLDAWQRDGSAAHIESLCRW"
     gene            2732..3535
                     /locus_tag="VEA63_05475"
     CDS             2732..3535
                     /locus_tag="VEA63_05475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HYD83476.1"
                     /translation="MAMRFCILGSGSSGNAAFLDTGETRVLVDAGFSARKLSQLLASI
                     GESLDRIDAVFLTHEHSDHSAGIEGLKKFPHLRLFANAATAAAIQTHLEHRPTWQLFE
                     TGGTFRFRDLEIRSFAVPHDAQEPVGFRFSHGHDDDLFTPRRSLTYLTDLGHAPQHIR
                     EHIRGCNVLVVESNHCAELLKNDPKRPWSLKQRISGRHGHLSNDAARALLAEVACPHW
                     RRVYLTHLSRDCNSRAAVETAFAPLRTTLATCEFSIVEHGQMTPLYDFA"
BASE COUNT          691 a         1558 c          967 g          641 t
ORIGIN      
        1 gctcgcgcaa ctcctcggcg ccatcttcgg ccccggccgc ggcttcgaac tcgtcagcaa
       61 cgtcaaccgc atccccaagg gctcgcgcct cgccgtctcc accaatctcc tcggcgcact
      121 catcggcgtc tgcatgcgcg ccaccagcca gatccgctcg ctcaccggcc cgctcgaaga
      181 atccgaacgc cgcatcgtcg ccgcccgcgc catcctcggc gaatggctcg gcggttccgg
      241 cggcggctgg caggattccg gcggtctctg gcctggcatc aaactcatcg agggcgcacc
      301 cgcgcaaccc ggcgacccgg aacacggcat ctcccgcggc acgctacttc cccgccacac
      361 actgctcggc gccgatcgca tctctcccga agcccgccag aaacttcagg actccctcgt
      421 cctcgtccat ggcggcatgg cgcaaaacgt cggccccatc ctcgagatgg ccacggagaa
      481 atatctcctc cgcagcgagg ccgaatggtc cgctcgtctc aagacggtcg ccacgctcga
      541 cgacatcctc gcgctcctcg ccgccggtga catcaagcag ctcggcgccg cgctcaccga
      601 aaactttttt ggcccgctcc aggcgatcat tccgtgggtg agcaatctct acaccgaacg
      661 cctcatcgag cgcacgcgcg ccgccttcgg cgaagacttc tggggcttct ggatgctcgg
      721 cggcatgtcc ggcggcggca tgggtttcat cttcgctccg catcgtaaac gcgaaggcca
      781 ggaaaaactt cgcgagatca tgctcacaac gaaacgcgaa ctcgaatcct cgctcccttt
      841 cgcgatggat ccggtcgtct acgatttcgc gatcaacgaa cacggcaccg tcgccgcact
      901 gctcaccggc cccgacgccc tgctgcccgc cgacttttat cgcgcgacga ttcccgccta
      961 ccttcgccgt tccgcccgcg agctcacgaa ccgcgaacgc ggcgatcttc aacaattcac
     1021 ccgcgccgcc cgcacgctcc ccgagttttc cgccgcgctc cccacgctcc tcgatcaact
     1081 gctcccctcc ggtgaatccc gcgacggcgg cagtcaacgt ctcgccgagc tcctcgcgca
     1141 aaacggcttc gatcgggcgc agcacgagca aatccgcgcc gacctccgca gcggccgcat
     1201 cggcctcgcg atgaaccgcc tccccgcgaa cacgctcatc gaagacgtcc ctcccaccgc
     1261 cctcttcaac tacgccgagg tagggacgcc tctccgaggc gtccgtcctg accccgcctc
     1321 ccccaaccca aaacccaaaa cccaaaactc agaactcgag gaattcgccg ccggcgaggc
     1381 ggccttgaaa cgcggcgaac tcgccgtcgt cacctacgcc gccggcgtcg gcagccgttg
     1441 gacccaaggc gcgggcgtcg tcaaaggtct gcatcctttc gccaaactcg ccggccgcca
     1501 tcgcagtttc atcgaaacgc acctcgcccg cacccgccac accgcgcgcg aattcggcac
     1561 cacgatcccg cacatcttca ccacgagtca tctcactcac gcgccgatcg aacactggct
     1621 ccgcatgcac gccgccgacg ccctcgaccg cgatgtcttt ctttctcccg gccgctcgat
     1681 cggcctccgt ctcgtccccg ccgtgcgcga tctccaattc gcctgggagg aaaccgcgca
     1741 gcaaaaactc gacgagcaaa aggagaagat gcgcgagagt atccgcgccg cccttacaaa
     1801 ttgggcgcgc accgtcggtg aaggcgccga ctacaccgac aaccttcccg agcagtgtct
     1861 ccaccctgtc ggccactggt tcgaaattcc gaaccttctc aaaaacggca cgctcgcccg
     1921 ccttctctcc gcgcagccgc aactccgcac gctcgtcgtt cacaacatcg acacgctcgg
     1981 cgcgtcgccc gaccccacgc tcctcggctg gtttcaatcc accggcgcca ccctcggctg
     2041 ggaagtgatt ccgaaacgca tcgaggatca cggcggtgga ctcgcgcgcg tcgacggccg
     2101 cctccgcatc gtcgaaggcc tcgcccttcc gcgcgaagac gacgagttca acctcacgta
     2161 ttacaacagc aacacctgct ggatcgacat cgaccgcttg ctcaccgttt tcggcctcac
     2221 acgcaaagac ctcgccgaca cggagaaagt cgccgccgcc gtccgcacgc tcgccgcgcg
     2281 cttgccaact tacgtgacgt tgaaagacgt aaagaaacgc tggggccacg gccaagagga
     2341 tgtgtatccg gttgctcaat tcgaaaaact ctggggcgac atgaccgccc tgccggagtg
     2401 cacgagcgtc ttcgccgccg tcccgagagc ccgcggccag caattaaaag accaagccca
     2461 actcgacgcc tggcaacgcg acggctccgc cgcccacatc gaatccctct gccgttggta
     2521 ggaccttggt agggacgcct ctccgaggcg tccgccccca cgcgtatccg ctcctatacc
     2581 atccgcggac ggctcggaga gccgtcccta cctttgccgc atccctccgc ttctcaaatc
     2641 cacgtttatc cgtgcccatc cgtggttaaa cttccgttgc caattttttc attggccccg
     2701 cgcccatcga ggtcttcaac cacctcgctc catggcgatg cgcttctgca ttctcggcag
     2761 cggcagttcc ggcaatgccg cctttctcga caccggtgaa acgcgtgtgc tcgtcgacgc
     2821 cggtttctcc gcgcgcaaac tttcgcaact cctcgccagc atcggcgaat cgctcgatcg
     2881 catcgatgcc gtcttcctca cccacgaaca cagcgatcac tccgccggca tcgaaggcct
     2941 gaaaaaattt ccgcacctcc ggctcttcgc caacgcggcc accgccgccg cgatccagac
     3001 gcacctcgaa caccgaccca cctggcagct cttcgaaacc ggcggcacgt ttcgcttccg
     3061 cgacctcgag atccgctcct tcgccgttcc acacgacgcg caggaacccg tcggcttccg
     3121 cttctcgcac ggtcacgacg acgacctctt caccccgcgc cgctcgctca cgtatctcac
     3181 ggacctcggt cacgccccgc agcacatccg cgaacacatc cgcggctgca acgtcctcgt
     3241 cgtcgaatcc aaccactgcg ccgagctcct caagaacgac cccaaacgcc cctggtcgct
     3301 caagcagcgc atcagcggcc gccacggtca tctctcgaat gacgccgccc gcgccctcct
     3361 cgccgaggtc gcctgcccgc attggcgccg cgtttacctc acgcacctgt cgcgcgactg
     3421 caattcccgc gccgccgtcg aaaccgcgtt cgcacccctg cgcaccaccc tcgccacctg
     3481 tgagttttcc atcgtcgagc acggccagat gacgccgctc tacgacttcg cttaacatcg
     3541 ggaaagttca ccgccgctcg tgtagcggca ggcctccgcg cctgcctgct ccgatcgtag
     3601 gttgtgcgct cgcgcgcaac gccacgcaac accacgcgac aacacgtcgt cgcccacctc
     3661 ctcctcggcc aacggcccac ccgctgcgcg ctcagcacgc cccacccgct gcgcgcaacg
     3721 ccgcttcggc caccgccccg cgagtctaca gcgtgacgac aaccacgctt gccgctccgg
     3781 cgcgtttcct gcacgccggt cggacgtcat gaccgttccc gccgacacct tccgccgcac
     3841 gaaaatcatc ttcaccc
//
LOCUS       DASPLT010000531         1418 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_129482, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000531 DASPLT010000000
VERSION     DASPLT010000531.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1418)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1418)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1418
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_129482"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1418
                     /locus_tag="VEA63_05480"
     CDS             <1..>1418
                     /locus_tag="VEA63_05480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD83477.1"
                     /translation="KIFATGFNCIQGLAWHGRDLWVANSPDLTIVRDLDGDDEADEYV
                     RVYTDLGNLEHAVHGLNWAPDGKLYLSAGTSKGLTQPPHRVAPKAFRDLWDVTAPEGT
                     PDLLPPRTYKKGDYKHTYQDPKDNWGREGGVLRADAMGANLEIVSRGTRNVWDIGFDH
                     EFNWLGTDNDQYDGDRIIMPFFGAHFGWGHTWSADWTGDQHLPTAPISGPVFQGSGTG
                     IVYYDSPQMPPEFRGVWFINDWLRKTTFVYRPTWDGALTMPDGGKWQEFALGRSALFQ
                     PVDIAIGPDGALYLTGWGKSYGAVYKNGEQVNEGRVFRISWPEATAANWNTPKRTRPI
                     AGWSVAELLEDLGSILPVWSTNAQEELVRRGSAVVPALTAALESRQLATAQETWALWT
                     LGRISLTDASIDAWFAQTGHTLSANAFIQSLRIAGHRWRASESAAPFPAFAVAALKDP
                     EPRVRFAAVQAIEQARQQKLIP"
BASE COUNT          268 a          521 c          389 g          240 t
ORIGIN      
        1 caagatcttc gccacgggct tcaactgcat ccaaggcctc gcgtggcacg gccgcgatct
       61 ctgggtcgcc aattcgcccg atctcaccat cgtccgcgat ctcgacggcg acgacgaagc
      121 cgacgaatac gtccgcgtct acaccgacct cggtaacctc gagcacgccg tccatggcct
      181 caattgggcg cccgacggca aactctatct ctccgccggc acttcgaagg gtctgacgca
      241 accgcctcat cgcgtcgcgc cgaaggcctt tcgcgatctc tgggacgtga ccgcgcccga
      301 aggcacgcct gacctcctgc cgccgcgcac ctataaaaaa ggcgactaca agcacacgta
      361 tcaggatccc aaggacaact ggggccgcga aggtggcgtg ctgcgggctg acgcgatggg
      421 agccaacctc gaaattgttt ctcgcggcac ccgcaacgtc tgggacattg gcttcgacca
      481 cgaattcaac tggctcggca ccgacaacga ccaatacgac ggcgaccgca tcattatgcc
      541 gttcttcggc gcccacttcg gctggggcca cacgtggagc gccgactgga ccggcgacca
      601 acatcttccc accgccccga tcagcggacc ggttttccaa ggctccggca ccggcatcgt
      661 ctattacgat tcccctcaaa tgccgcccga gtttcgcggc gtctggttca tcaacgactg
      721 gcttcgcaaa accaccttcg tttaccgccc gacttgggac ggcgcgctca cgatgcccga
      781 tggtggaaaa tggcaggagt tcgcgcttgg acgttccgcc ctcttccagc ccgtcgacat
      841 cgcgatcggt cccgacggcg cgctctacct cacgggctgg ggaaaatcct acggcgcggt
      901 ctacaaaaac ggcgaacagg tcaacgaagg ccgcgtgttc cgcatctcct ggcctgaagc
      961 gaccgcggcc aactggaaca cgcccaaacg cacgcgtccc atcgccggct ggagcgtcgc
     1021 ggaattgctc gaagatctcg gctcgattct ccccgtctgg agcaccaacg cccaggagga
     1081 actcgtccgc cgcggctcgg ccgtcgtgcc cgcgctcacc gccgcgctcg agtcccgcca
     1141 actcgccacc gcgcaggaaa cgtgggcgct gtggaccctc ggccgtatca gtttaacgga
     1201 tgcgtcgatt gacgcgtggt tcgcccaaac cggtcacaca ctctcggcca acgcgttcat
     1261 tcaatccctc cgcatcgccg gccaccgttg gcgcgccagc gaatccgccg ctcctttccc
     1321 cgccttcgcc gtcgcggcat tgaaagaccc cgaaccgcgc gtgcgtttcg ccgccgtcca
     1381 agcgatcgaa caagcgcgcc agcaaaaact cattcccg
//
LOCUS       DASPLT010000532         1265 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_88736, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000532 DASPLT010000000
VERSION     DASPLT010000532.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1265)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1265)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1265
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_88736"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..714
                     /locus_tag="VEA63_05485"
     CDS             <1..714
                     /locus_tag="VEA63_05485"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009508955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA adenine dimethyltransferase family protein"
                     /protein_id="HYD83478.1"
                     /translation="VEIGPGLGTLTSALLEAGAEVWAVEKDRTMHEHLAQTLVPRYPQ
                     TFHLLEGDAVEHPLAGFAAESSPSSGSPGSPRDFKIVANLPYAIATPWLDAVLSGPLP
                     ARLVLMLQQEAAQRYVAQPGTKAFGAISIFLQSAFAVAPGHKVEASCFYPRPDVDSYL
                     LHLVRRETPFIFPTAVKVMIRACFQQRRKQIGGLLRDRLPDHGRGWLAKLEAAGFPPQ
                     TRPEAIPTELWQTLPGSML"
     gene            949..>1265
                     /locus_tag="VEA63_05490"
     CDS             949..>1265
                     /locus_tag="VEA63_05490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD83479.1"
                     /translation="MSRFLLPLLLLISAPLFAQQPAAPNSLSGRVEGRTYISPTGAFR
                     VTVPVLPELGGRIMDTANVVTFQDSFSVLCTIAAFPMDATQRWEHSTRGPKDYLAYFF
                     ANFV"
BASE COUNT          222 a          430 c          343 g          270 t
ORIGIN      
        1 gtcgaaatcg gtcccggcct cggcacgctt acgtccgcgc ttctcgaagc cggcgccgaa
       61 gtctgggcgg tcgaaaaaga ccgcaccatg cacgagcacc tcgcgcaaac gctcgtgccg
      121 cgttacccac aaacctttca cctgctcgaa ggtgacgccg tcgagcaccc gctcgccggt
      181 ttcgctgccg agtcttcacc ttcgtccggc tcgcccggat ctccgcgcga tttcaaaatc
      241 gtcgcgaacc tcccctatgc gatcgcgacg ccgtggctgg acgcggtgct ctccggtccc
      301 ttgcccgctc gcctcgtgtt gatgcttcaa caggaagccg ctcaacgcta cgtcgcgcag
      361 cccggcacga aagcgttcgg cgcgatctcg atttttctgc aatccgcttt cgctgtcgcg
      421 cccggtcaca aggtcgaagc gtcgtgtttt tacccgcggc cggacgtcga ttcctacctg
      481 ctgcatctcg ttcgccgcga aacgccgttc attttcccga cggcggtgaa ggtgatgatc
      541 cgcgcgtgct ttcaacaacg gcggaaacag atcggcgggc tgctacgcga ccgccttccc
      601 gatcacggcc gcggatggct ggcgaaactc gaagcggccg gttttccccc acaaacccgc
      661 cctgaggcga ttccaacgga actttggcaa acgcttccgg gtagcatgct ctgagccgga
      721 ttcatgcagt tgagaaacca cgaatggaca cgaatgaaca cgaatgagaa atcaggacga
      781 agcgtggtta taagccggtc accgttcgct cgtcgaccga tcatctcggc tgctgctacg
      841 tggttcttct gccattcgtg ttcattcgtg tccattcgtg gttcttccat gcagggtccc
      901 acctaaggcg cttgaaagcg tcgcgagggc gcctagtgat atttccccat gtcccgtttc
      961 ctcctcccgc tccttttgtt gatcagcgcc ccgctgttcg cacaacagcc ggccgcccca
     1021 aattcgctct ccgggcgtgt cgaggggaga acttacattt ctcccaccgg cgcgtttcgc
     1081 gtcacagtgc ccgtgctacc cgaactcggt ggccgcatca tggacacggc caacgtcgtc
     1141 acttttcagg acagcttcag cgtgctctgc acgatcgccg cgttcccgat ggacgcgacg
     1201 caacgctggg aacactcgac gcgcggcccg aaagactacc tcgcgtattt cttcgcgaat
     1261 ttcgt
//
LOCUS       DASPLT010000533         1124 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_293634, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000533 DASPLT010000000
VERSION     DASPLT010000533.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1124)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1124)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1124
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_293634"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(447..>1124)
                     /locus_tag="VEA63_05495"
     CDS             complement(447..>1124)
                     /locus_tag="VEA63_05495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007364095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6596 domain-containing protein"
                     /protein_id="HYD83480.1"
                     /translation="NEGYKASAGERLLREDLCQEAVRLTSLLVEHPAGDTPRSHALLA
                     LMLLTAARFPSRIDEDGDLLRLDDQDRSKWNPVLIERGLIELVAAAQGDELSEYHLQA
                     GIAACHCTATDYASTNWSRILRHYDELNRMKPSPVVALNRAVAVAQLHGPQAGLEAID
                     AIPQRERLESHYLLHAVTGELHWRRKDHRAAAESFRRAVQLAKVGPEQLYLTRMLERA
                     DPAEASA"
BASE COUNT          211 a          325 c          370 g          218 t
ORIGIN      
        1 aacgaagagg aggttgattg atggttcgct gtttcaacga atgaacccgg cggtttcgga
       61 cgcgtttgaa aacggatcct tttattttcg ccgcgctcct tgcgcgtccg ggcggactag
      121 cgcgggaaat tcacacctgt agcgaaaagc gcatctgcag gtgataaatg cagaccaagc
      181 gagaagtagt ttagaacaga tcgatcaaaa ctatggccgc aaaaaagcac cgaagacgca
      241 aaaacggctt ggaggactct tctgttcttg cgccttttgc gcgtctttgc ggccaaagag
      301 gttggctgta gaatttgaag cccggtcagc gatatgacgc taggccgcga tcgctgcgcg
      361 tcatcgcctg caaacaggcg cccacctccg gcgtctcgat cggttataat aatgcaaacg
      421 gctccaacac tccagcggac gctcgctcag gccgaggcct cggccgggtc ggcccgctcg
      481 agcatgcgcg tgaggtagag ttgctccggg ccgaccttgg caagttgcac ggcgcggcga
      541 aagctttccg ccgcggcgcg gtgatccttc cgtcgccaat gtaattcgcc ggtgacagca
      601 tgcagcaggt agtgcgactc gaggcgctcg cgttgcggaa tcgcgtcgat cgcttcgagc
      661 cccgcctgcg ggccgtgtag ttgcgcgacg gctacagcgc ggttcagcgc cacgacgggg
      721 gacggcttca tccggttgag ctcgtcgtag tgccggagga tgcgcgacca gttcgtcgaa
      781 gcgtagtcgg tcgcggtgca atgacacgcg gcgatgccgg cttgaagatg atactcgctc
      841 aactcgtcgc cttgcgcggc ggcgacgagc tcgatcaagc cgcgctcgat cagcacggga
      901 ttccatttcg agcgatcctg gtcgtcgagt cggagcaggt cgccgtcttc gtcgatccgc
      961 gatgggaatc gtgcggcggt gagcagcatg agcgcgagca gcgcgtggct gcgcggcgtg
     1021 tcgccggcgg gatgctcgac gagcagcgag gtcaaccgca cggcttcctg gcagagatcc
     1081 tcgcgcagca ggcgttcgcc ggccgaggct ttgtagcctt cgtt
//
LOCUS       DASPLT010000534         1755 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_6852, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000534 DASPLT010000000
VERSION     DASPLT010000534.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1755)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1755)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1755
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6852"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1522)
                     /gene="ligA"
                     /locus_tag="VEA63_05500"
     CDS             complement(<1..1522)
                     /gene="ligA"
                     /locus_tag="VEA63_05500"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF005932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HYD83481.1"
                     /translation="MDAAEAQKRIAELRSQVGRHDELYYRQAKPEITDFDYDLLKQEL
                     ADLETRFPKLAGGDSPTKRIGDDRVRGFKEVEHRQKMLSLDNTYNEAELRAFHARLAR
                     LLEADDLRYTVEPKIDGLAVSLTYEDGRLVRAVTRGRGDRGDDVTANVLTIEALPRML
                     KGAAPKIVEIRGEIYLTQAEFERINAERTAAGEELYANPRNTAAGTIKQLDPAEVARR
                     KLSIVLYSLGYREPEVVSSQTELQDKLRAWGLPVVERYWKVRGIDEAWAAIGELDGMR
                     RAFAYGTDGAVVKLDDFEQQRIVGSTDKSPRWAIAYKYKPDTARTRLNAITIQVGKTG
                     ILSPVAELEPVFLSMSTIARATLHNENEIRVKDIRVGDLVEIERAGEVIPAVLRSFPE
                     ERPAGAAEFDLFAAVGGKCPVCGGRIAKVEVDTETGVGAAWKCQNYDCRAQRVGRLVF
                     FCSRRALAIDGVGDIVAAKLVELDLVKDPPDLYDVSFETLATLNLGTAEEPRVFGEK"
     gene            complement(1509..>1755)
                     /locus_tag="VEA63_05505"
     CDS             complement(1509..>1755)
                     /locus_tag="VEA63_05505"
                     /inference="COORDINATES: protein motif:HMM:NF016763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HYD83482.1"
                     /translation="AILIVSVFGVTDELHQHFTPGRSMDVWDWVADTLGAIVATVLYA
                     EWAAYRRLLETPVFGLFRKRRVEIADAACVIAADGRR"
BASE COUNT          327 a          684 c          411 g          333 t
ORIGIN      
        1 ttttttcacc gaacacccgc ggctcctccg ccgtgccgag gttcagcgtc gcgagcgttt
       61 cgaaactcac gtcgtaaaga tccggcggat ccttcaccaa atccagctcc accaatttcg
      121 ccgccacgat gtcgcccacg ccatcgatcg ccagcgcacg acggctgcag aaaaacacca
      181 gccgacccac gcgctgcgcg cgacaatcgt agttctgaca cttccacgcc gcgccgacgc
      241 ccgtctcggt gtccacttcc accttcgcga ttcttccacc gcacaccgga cacttccctc
      301 caaccgccgc gaacaaatcg aactccgccg ctcccgccgg ccgctcctcc ggaaacgagc
      361 gcaacaccgc cggaatcact tcgcccgcgc gctcgatctc caccagatcg cccacgcgaa
      421 tatccttcac ccgaatctcg ttttcgttgt gcagcgtcgc ccgcgcgatc gtgctcatcg
      481 ataaaaacac cggctcgagt tccgccaccg gactaaggat tcccgtcttt cccacctgaa
      541 tcgtgatcgc attcaaccgc gtccgcgccg tgtccggttt gtatttataa gcgatcgccc
      601 agcgcggcga tttgtccgtc gagccgacga tccgctgctg ctcgaaatcg tccaacttca
      661 ccaccgcgcc atctgtcccg taagcaaacg cccgccgcat cccatcgagt tcgccgatcg
      721 ccgcccacgc ttcgtcgatc ccgcgcactt tccaataacg ctcgaccacc ggcagtcccc
      781 acgcgcgcaa tttatcctgc aactccgtct gcgagctcac gacttccggc tcacgataac
      841 cgagactata cagcacgatc gaaagtttcc gccgcgccac ctccgccgga tcgagctgct
      901 tgatcgtgcc cgccgcggtg ttgcgcggat tcgcataaag ttcctcgccc gccgccgtcc
      961 gctccgcgtt gatccgctcg aactccgcct gcgtcagata aatctctccg cgaatctcca
     1021 cgatcttcgg tgccgcgcct ttcaacattc gcggcaacgc ttcgatcgtc agcacgttcg
     1081 ccgtcacatc atcgccccga tcgccgcgcc cgcgcgtcac cgcgcgcacg agccgcccgt
     1141 cctcatacgt caggctcacc gcgagcccgt cgatcttcgg ctcgaccgtg taacgcagat
     1201 catccgcctc gagcaaccgc gcgagccgcg cgtgaaacgc ccgcagctcc gcctcattgt
     1261 aagtgttatc caagctcagc attttctgcc gatgctcgac ttctttaaac ccgcgcacgc
     1321 gatcgtcgcc gatccgcttc gtcggggaat cgccgcccgc cagcttcgga aaccgtgtct
     1381 ccaaatccgc caactcctgc ttcagcaggt catagtcgaa gtcggtgatc tccggcttcg
     1441 cctgccggta atacagttcg tcgtgccggc cgacttgcga ccgaagttcc gcgatccgtt
     1501 tctgagcttc agcggcgtcc atccgcggcg attacgcatg ccgcgtccgc gatttcaacg
     1561 cgccgtttgc gaaataaccc gaacaccggc gtctccagca aacgccggta agccgcccac
     1621 tccgcataaa gcaccgtcgc cacgatcgcg cccagcgtgt ccgccaccca atcccataca
     1681 tccatcgagc ggcccggcgt gaaatgctga tgcaactcat ccgtcacgcc gaacaccgac
     1741 acgatcagaa tcgcc
//
LOCUS       DASPLT010000535         1403 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_129560, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000535 DASPLT010000000
VERSION     DASPLT010000535.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1403)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1403)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1403
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_129560"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..670)
                     /locus_tag="VEA63_05510"
     CDS             complement(<1..670)
                     /locus_tag="VEA63_05510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HYD83483.1"
                     /translation="MNSADLPVAILAGGLATRLRPITESIPKLLVDVAGEPFFTHQIR
                     LLKAASLTRLVLCIGYLGERIVELYGDGSRHGVQIDYVFDGPKLLGTGGALIQALPQL
                     GDAFYVLYGDSYLPINYTEVGDAFLRSGRQGLMTVYENRERYDTSNVWFEAGRIRLYD
                     KKNKLPQMQHIDYGLGVFRASAFNDFARDAVIDLADVQKSLVQRNELAGYEVTQRFYE
                     IGSPS"
     gene            complement(696..>1403)
                     /locus_tag="VEA63_05515"
     CDS             complement(696..>1403)
                     /locus_tag="VEA63_05515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HYD83484.1"
                     /translation="DIPGGTGLGSSGSFTTALLKALHASKKNIVSPADLAAQACEIEL
                     DRLGEPIGKQDQYIAAIGGITAFTFHRDGRVEYRPVRLSEETLYNLEDNLLLFFTGYS
                     RSASAILKDQNDRSKQQDSAMLENLHFTKELGYKSLECLESGNLEEFARLMDVHWQRK
                     KARSSGMSNAHINDWYDFAMQNGALGGKLIGAGGGGFLMFYAADKPKLRHAMRAKGLQ
                     EVRFRFDFEGSKVVAQD"
BASE COUNT          257 a          380 c          482 g          284 t
ORIGIN      
        1 cggagggcga cccgatttcg tagaaccgct gcgtgacctc gtagccggca agttcgttgc
       61 gttgcacgag ggatttctgg acgtcggcga gatcaatcac ggcgtcgcgg gcgaaatcgt
      121 tgaaggcgga agcgcgaaac acgccgagcc cgtagtcgat gtgctgcatc tgcgggagct
      181 tgttcttctt gtcgtagagg cggattcgac cggcctcaaa ccagacgttg ctcgtgtcgt
      241 aacgctcgcg gttttcgtaa acggtcatca agccctgtcg gccggagcgc agaaaggcgt
      301 cgccgacctc ggtgtagttg atcggcagat acgagtcgcc gtagagcacg taaaaagcgt
      361 cgccgagctg cggcagcgcc tggatcaacg cgccgccggt gccgaggagt ttcgggccat
      421 cgaagacgta gtcgatctga acgccgtggc gcgagccgtc gccgtagagc tcgacgatcc
      481 tttcgccgag ataaccaatg cagagcacga ggcgggtgag gctggcggct ttgagcaggc
      541 gaatctggtg agtgaagaac ggctcgccgg cgacatcgac gaggagtttc ggtatgcttt
      601 ccgtgatcgg ccgcaggcgc gtggcaagtc caccggcgag gatcgcgacg gggaggtcgg
      661 cggagttcat cgggaaggat tgggatgcgg catgcctagt cctgtgcgac gaccttgctg
      721 ccttcgaagt caaagcggaa gcggacttcc tggagccctt tcgcgcgcat cgcgtgacgg
      781 agtttcggtt tgtcggcggc gtagaacatc aggaaaccac cgccgccggc gccgatgagt
      841 tttccgccga gcgcgccgtt ttgcatcgcg aagtcatacc agtcgttgat gtgcgcgttg
      901 ctcatgccgg aggagcgcgc ctttttgcgc tgccagtgca cgtccatgag acgcgcgaac
      961 tcctcgagat tccccgactc gaggcattcc agagatttgt agccgagctc tttcgtgaaa
     1021 tggaggttct caagcattgc gctgtcctgt tgtttcgaac ggtcgttctg atccttgaga
     1081 atcgctgagg cgctgcgcga gtagccggtg aagaagagga gcaggttgtc ctcaagattg
     1141 tagagggttt cctcggagag gcggaccggg cggtattcga cgcggccgtc gcgatgaaag
     1201 gtgaacgccg tgatgccacc gatcgcggcg atgtattggt cttgtttccc gatgggctcg
     1261 ccgagccggt cgagctcgat ttcgcaggcc tgagcggcga gatcggcggg cgaaacgatg
     1321 tttttcttcg aggcgtgcaa ggccttcagc agcgcggtgg tgaaacttcc cgacgagccg
     1381 agtccggtgc caccggggat gtc
//
LOCUS       DASPLT010000536         1403 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_272284, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000536 DASPLT010000000
VERSION     DASPLT010000536.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1403)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1403)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1403
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_272284"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(90..863)
                     /locus_tag="VEA63_05520"
     CDS             complement(90..863)
                     /locus_tag="VEA63_05520"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013980.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HYD83485.1"
                     /translation="MSVEQQEDLFGQFAPEPGVHKAPPAAAPAPAPTANRDGVVFQRS
                     DRARGYRLTLKRDGTAVATIPIRGSEREARRFVEQHQAWLERARERQRHKPRGADVWA
                     VGTHVLWRGEMTEIRVASTAGDRPQVCLAADVFRVAKLDGDLRPTLESHFMRRAKIEL
                     PARTWELAAVTGVDVKQVVVRNQRSRWGSCSANGTISLNWRLVQTPEFVRDYIVYHEL
                     MHLREMNHSARFWARVEEVCPGWQDAERWIKRNGSLVGL"
     gene            complement(900..>1403)
                     /locus_tag="VEA63_05525"
     CDS             complement(900..>1403)
                     /locus_tag="VEA63_05525"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HYD83486.1"
                     /translation="NRGWFAGVFATALLTDVLDGFLARWLHAYSDFGRKLDSIADYVT
                     LFTGLACLGLLWPDVVRNEWPWFAAVLGSFFVAQAFCFWRLGRAPCYHTWASKATVAG
                     CVIALVPLLAGWTPVPARTVAVFQVLVGFEEMAIAVLVPWHTGEMPSVWHAWRRRRER
                     ERTQVES"
BASE COUNT          262 a          566 c          365 g          210 t
ORIGIN      
        1 ttagtggtga agcagacgct ttctccggag atgcctgccg gctcatctac gaggaagaac
       61 tcgacgaaga ccgctgaggt cgcccttcgt cagagcccca ccagcgagcc gttgcgcttg
      121 atccagcgct ccgcgtcctg ccaccccggg caaacctctt ccacgcgcgc ccaaaaacgc
      181 gcggagtgat tcatttcccg caggtgcatc agctcgtggt aaacgatgta gtcgcgcacg
      241 aactccggcg tctgcaccag ccgccagttt agcgaaatcg ttccgttcgc cgagcacgag
      301 ccccagcgcg aacgctgatt gcgcacgacg acctgcttca cgtccacgcc cgtgaccgcc
      361 gccaactccc acgtgcgcgc cggcaattca atcttcgccc gccgcataaa atgcgactcc
      421 aacgtcggac gcagatcgcc gtcgagcttc gccacccgaa acacatccgc cgccaaacac
      481 acctgcggtc ggtcgcccgc cgtcgacgcc acacgaatct ccgtcatctc cccgcgccac
      541 agcacatgcg tgcccaccgc ccagacgtcc gcgccgcgcg gcttgtgccg ctggcgttcg
      601 cgcgcccgct cgagccacgc ttgatgctgc tccacgaacc gccgcgcctc gcgctccgag
      661 ccgcgtatcg ggattgtggc gacagccgtg ccgtcccgtt tcaacgtcaa ccgatacccg
      721 cgcgcgcgat cgctccgttg aaacaccacg ccgtcgcgat tcgcggtcgg ggcgggtgcc
      781 ggcgccgcgg ccggcggcgc tttgtgcacg cccggctccg gtgcgaattg cccaaacaaa
      841 tcctcttgct gctccacgct catgcgtcgc cgccgagcct catcaacgcc gcccccgcgt
      901 caactctcca cctgtgttct ttcccgctcc cgccgccgtc tccacgcgtg ccacacactc
      961 ggcatttcgc ccgtatgcca cggcaccagc accgcgatcg ccatctcctc gaagcctacc
     1021 agcacctgaa acaccgccac cgtccgcgcc ggcaccggag tccatcccgc gagcagcggc
     1081 accagcgcga tcacgcaccc cgcgaccgtc gccttcgagg cccacgtgtg ataacacggc
     1141 gcgcgcccga gccgccaaaa acaaaacgcc tgcgccacga aaaacgaacc cagcaccgcc
     1201 gcaaaccacg gccattcgtt tcgcaccacg tcgggccaca acaacccgag gcatgccaat
     1261 cccgtgaaca acgtcacgta gtccgcgatg ctgtcgagct tgcgtccaaa atctgaatac
     1321 gcatgcagcc accgcgcgag aaacccgtcc agcacgtccg tcagcagcgc cgtcgcgaac
     1381 acgcccgcga accacccgcg gtt
//
LOCUS       DASPLT010000537         1045 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_107964, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000537 DASPLT010000000
VERSION     DASPLT010000537.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1045)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1045)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1045
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_107964"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..294)
                     /locus_tag="VEA63_05530"
     CDS             complement(<1..294)
                     /locus_tag="VEA63_05530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD83487.1"
                     /translation="MSTLLPEKNARATRLPRTAGQFARCLGIALACAFALSAGEAHEF
                     PDIPADFLQEAESIPDFWVSEVDAIEQFLRQQVKRGEVEIVGRTAGGRPIRAVA"
     gene            complement(368..>1045)
                     /locus_tag="VEA63_05535"
     CDS             complement(368..>1045)
                     /locus_tag="VEA63_05535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HYD83488.1"
                     /translation="GAWTREPEAAVLAHCRRVAAELPLIGFYLQPAVGGRVFSYRFWR
                     AFAEIPELVAVKLAPFDRYRTLDVVRAVLEADREDVALYTGNDDNIIADLLTPFEFAG
                     RTRHFNGGLLGQWGVWTERAVALFRDLQRARARGRVDLQWLTRNAALTDANAAIFDSA
                     NRFAGCLPGIHEVLRRQGLLATTACLDPHEQLSPGQAREISRVSAAYPWLVDDAFVEQ
                     HRDTWLS"
BASE COUNT          205 a          343 c          336 g          161 t
ORIGIN      
        1 cgccaccgcg cggatcggcc ggccgccagc ggtgcgaccc acaatctcca cctcgccgcg
       61 cttcacctgc tggcgaagaa actgttcgat cgcgtcgact tcgctcaccc aaaaatccgg
      121 aatcgattcc gcctcctgca gaaaatcggc ggggatgtcg gggaattcgt gagcctcgcc
      181 ggcggacaac gcgaaggcgc aggcgagagc gattccgagg caacgggcga actgcccggc
      241 ggtgcgcggg agacgggtgg cccgcgcgtt tttttcaggg aggagtgtgc tcataaattc
      301 gagggtctaa gttcgatgca ggactacgga cgattgaatg ggtgaaaaga aaatgcgcga
      361 tgtcgtgtta ggaaagccag gtgtcgcgat gctgttcgac gaacgcatcg tccaccagcc
      421 acggatacgc cgccgagacg cggctgattt cacgggcctg cccgggcgac aactgctcgt
      481 gcggatcgag acacgccgtg gtcgcgagca aaccttgccg ccgcagcacc tcgtggatgc
      541 ccggcagaca tcccgcgaaa cggttcgcgc tgtcgaagat cgcggcgttt gcatcggtca
      601 gcgcggcgtt gcgtgtgagc cactgcaaat cgacgcgtcc gcgcgcccgc gcgcgctgca
      661 agtcgcggaa gagcgcgacc gcgcgctccg tccacacgcc ccactgcccg agcagcccgc
      721 cgttgaaatg tcgcgtgcgg cccgcgaact cgaagggcgt gagcagatcc gcgatgatgt
      781 tgtcgtcatt gcccgtataa agcgcgacgt cctcgcgatc ggcctcgagc accgcgcgca
      841 cgacatcgag cgtccgataa cgatcgaatg gcgcgagctt caccgcgacg agttcaggga
      901 tctccgcaaa cgcgcgccag aagcgatagc tgaacacgcg tccgcccacc gccggctgaa
      961 gataaaaacc gatcagcggc agctccgcag cgactcgcct gcagtgagcg agcaccgccg
     1021 cttcgggttc gcgcgtccac gctcc
//
LOCUS       DASPLT010000538         1183 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_149763, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000538 DASPLT010000000
VERSION     DASPLT010000538.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1183)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1183)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1183
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_149763"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..580)
                     /locus_tag="VEA63_05540"
     CDS             complement(<1..580)
                     /locus_tag="VEA63_05540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HYD83489.1"
                     /translation="MRAGLAGLIDRQPDMVVGAEAGSPAEAFQQLPKIKPDLVLTDLT
                     MPGRSGLEFIKDLRAAEPALAILVISMHDEAVHAERALRAGARGYIMKEAGGENLLTA
                     IRQVLRGEVYVSPRMSARILEGLSGARPRGSSSPIEKLTDREFEIFQLIGQGKSTRDI
                     AEQLHLSPKTVDVHRSHIKEKLDLKDVTALIRH"
     gene            complement(938..>1183)
                     /locus_tag="VEA63_05545"
     CDS             complement(938..>1183)
                     /locus_tag="VEA63_05545"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HYD83490.1"
                     /translation="VNNAVKHARSTTVTISLIRESDALVLTVSDDGSGLARNAAKSRG
                     LGLGVMQHRAKVIGADLTVNSKRGDGVTIHCRLPLAR"
BASE COUNT          198 a          324 c          437 g          224 t
ORIGIN      
        1 cgtggcgaat gagcgcggtc acgtccttga ggtcgagctt ctccttgatg tggctgcgat
       61 gcacgtcgac cgtcttcggg ctgaggtgaa gctgctctgc gatgtcgcgc gtgctcttgc
      121 cctgaccgat gagttggaaa atttcgaact cgcggtcggt caatttttcg atcggcgaac
      181 tcgaaccgcg ggggcgcgcg ccggaaaggc cttcgagaat ccgcgccgac atgcgcgggc
      241 tcacatagac ctcgccgcgc agcacctggc ggatggcggt gaggagattt tcgccgccgg
      301 cttccttcat gatgtagccg cgcgcgccgg cgcggagcgc gcgctcggcg tgaacagctt
      361 cgtcgtgcat tgagatcacg aggatcgcga gagccggttc ggcggcgcgc aggtctttga
      421 tgaattcgag tccgctgcgg ccgggcatcg tcaaatcggt caacaccaag tccggtttga
      481 tcttcggcag ttgttggaat gcctcggcgg ggctgccggc ttcagcgccg acgaccatgt
      541 cgggctgccg atcgatgaga ccggcgagac cggcgcgcat gaacgggtga tcgtcgacga
      601 gaaggatgcg tttgcgcgtg gatgcggagg aaggggcgga cgcactcgga gacggagcag
      661 aagcgggagc aggagtagga cgggcgcgag atttgtctgc gggtggcttc atgcgggcta
      721 agcggtaagg gcgggcggtg cagagagtag ggcgggcttc agcgcgaata tttcacactc
      781 actggaatat gcctcacgaa gtgatttcgg aaaggtaggg gcggctctcc gagccgtccg
      841 cggatgcctc ggagaggcat ccctaccttt gcgccagttg cgcggtgcgg cggcaccaag
      901 tggcgggctg aagcccgccc tactttttgg gggcgactca tcgggcgagc gggaggcggc
      961 agtggatggt gacgccgtca ccgcgtttcg aattcacggt caagtcggcg ccgatcacct
     1021 tggcgcgatg ttgcatgacg ccgaggccga ggccgcgcga tttcgcggcg ttgcgcgcaa
     1081 ggccggaacc atcgtcgctg acggtgagca cgagcgcgtc ggactcccgt atcaggctaa
     1141 tggttacggt ggtggagcgg gcgtgtttga cggcgttgtt cac
//
LOCUS       DASPLT010000539         2251 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_272388, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000539 DASPLT010000000
VERSION     DASPLT010000539.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 2251)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2251)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2251
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_272388"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            114..1628
                     /locus_tag="VEA63_05550"
     CDS             114..1628
                     /locus_tag="VEA63_05550"
                     /inference="COORDINATES: protein motif:HMM:NF042648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N,N-dimethylformamidase beta subunit family
                     domain-containing protein"
                     /protein_id="HYD83491.1"
                     /translation="MRDLPLTSQSARGPASAIRRVLPVAALLAVLLFPFAAGAADSSI
                     VSENQRRGTKDWLLTKVDAVLVQKNVLGEDSPHFARSKRVEGFASQTSYRAGDKATFF
                     ISAEPASNVAIDIYRMGYYQGNGGRHVRSVDAIPVKPQPTPDDGERNLRECKWSPAHE
                     FTVPSDWLSGVYLAKLTRADDGFQSYIVFVVRDDRKADFNFQVSDMTWQAYNRWPAWR
                     SLYDFQGKLWNTSGGDIVSFDRPYTFFYNLLPSAANPLTNGSGEFLLWELPLAFWMEE
                     QGYDVTYTSTRDTHRDAPGLLRTKAFLSVGHDEYWTRQMYDNVARARDEGVNLLFLSG
                     NAVDGEVFLTTSTDGRPDRIMGRARWFFDEEKLMGASSYGVGLGDWVVTQPDHWLYAD
                     TGMKKGDTIADLVGWEYHGPPLKDDPTLVVVAEGKFTDFRWEAESTHAATFYDGPKGN
                     FVFNAGTCWWSMPLARPPGAVNPPDADFSKTDPRVQQMTKNLFERVVNAQSRTE"
     gene            1733..>2251
                     /locus_tag="VEA63_05555"
     CDS             1733..>2251
                     /locus_tag="VEA63_05555"
                     /inference="COORDINATES: protein motif:HMM:NF025861.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 97 N-terminal
                     domain-containing protein"
                     /protein_id="HYD83492.1"
                     /translation="MKPAQMMPLSFRIATFLAVVGLAFTSRAAEPSLGETWTVLSPDQ
                     THELLVTLSAEGRLAYALNRRGTDVSVPVLGASPLGLATDAGRFVDRLKIVSASEPTQ
                     VDEAYAMATGKQLALRDRHTAWSLELANVDEHPFAVDFRVYEDGLAFRYRLPQLAKPT
                     ARVVGEHSGFRFA"
BASE COUNT          428 a          768 c          626 g          429 t
ORIGIN      
        1 caggtaaaac gccggagtgc acggcgcgga ctatcccgcc caacggttcg agaacgggtt
       61 cacccggcgt gccgttgcgg cccaacccgg atcggagaat ggatttccct gccatgcgtg
      121 atctccccct cacctcgcaa agtgcccgcg gaccggcgtc tgcgattcgc cgcgttttac
      181 ctgttgccgc actgctggcg gtcctccttt tcccgtttgc ggcgggcgcg gcggacagtt
      241 ccatcgtatc cgaaaatcag cgacgcggga ccaaggactg gcttctcacc aaggtcgacg
      301 cggtcctcgt gcagaaaaac gtgctcggcg aagacagccc gcacttcgcg cgcagcaaaa
      361 gagttgaagg ctttgcctcg cagaccagct atcgcgcggg cgacaaagcc acgttcttca
      421 tcagcgctga gccggcgagc aatgtcgcga tcgatatcta tcgcatgggt tattatcagg
      481 gaaacggcgg acgccatgtt cgctccgtcg atgccattcc ggtcaaaccg cagcccactc
      541 cggacgacgg cgaacgcaac ttgcgcgaat gcaaatggtc gcccgcgcac gagttcaccg
      601 ttccctccga ctggttgagc ggcgtctatc tcgccaagct tacccgcgcc gacgacggct
      661 ttcagtccta catcgtcttc gtcgtgcgcg acgaccgaaa ggccgacttc aatttccaag
      721 tctccgacat gacgtggcag gcctacaatc gctggcccgc gtggcgctcg ctctatgatt
      781 ttcaaggcaa actctggaac acctccggcg gcgacatcgt cagcttcgac cggccttaca
      841 cgtttttcta caacctcctt ccctccgctg ccaatccgct gacgaacggc tccggcgaat
      901 ttctcctgtg ggaacttcca ctcgcgttct ggatggaaga acaaggctac gacgtcacct
      961 acacgtccac gcgcgatacg caccgcgacg cgccaggtct gctccgcacg aaggcgtttc
     1021 tctccgtcgg ccacgacgaa tactggacgc gccagatgta tgacaacgtc gctcgcgccc
     1081 gcgacgaagg cgtcaacctc ctcttccttt cgggcaatgc ggtcgatggc gaagtgtttc
     1141 tcaccaccag cacggacggt cgcccagacc gcatcatggg tcgcgcgaga tggtttttcg
     1201 acgaagagaa actcatgggc gcgtcctcct acggcgtcgg ccttggcgat tgggtcgtca
     1261 ctcagccgga tcactggctc tacgccgaca ccggtatgaa aaaaggtgat acgatcgccg
     1321 acctcgtcgg ctgggaatat cacggtccgc cgttgaagga cgatcccacg ctcgtcgtcg
     1381 tggcggaggg aaagttcacg gacttccgtt gggaagcgga gtccacgcat gccgcgacat
     1441 tttacgacgg cccgaaaggc aacttcgtct tcaacgccgg cacctgttgg tggagcatgc
     1501 cgctcgcgcg tccgcctggc gcggtgaacc caccggacgc tgacttcagc aaaaccgatc
     1561 ctcgcgtgca gcagatgacg aaaaatctct tcgaacgcgt ggtgaacgcc cagtcgagaa
     1621 cagagtagtt gcagcaaatc aattatggcg ctgcgcgcga gcgcagggat ttccgctgcg
     1681 atcaaccaat cttctcccgc tgctcacgcc agcgagccac gcccacgaca tcgtgaaacc
     1741 cgctcaaatg atgcctctct cttttagaat cgccacattt ctggccgtcg tcggtctcgc
     1801 gttcacgtct cgcgcggcgg agccctcgct cggtgaaaca tggaccgtcc tctcgcccga
     1861 ccaaacccac gagcttctcg tgacactttc cgccgaagga cgactcgcct atgcgctgaa
     1921 ccgccgcggc accgacgtct cagtccccgt gctcggcgcc tcgccgctcg gcctcgcgac
     1981 cgatgccggc cgtttcgtcg accggctcaa aatcgtttcc gcgagcgaac cgacgcaagt
     2041 cgacgaagct tacgcgatgg cgaccggcaa gcagctcgcg ctgcgcgatc gccacaccgc
     2101 ctggtcgctc gaactcgcga acgtcgacga gcatccgttc gcggtcgact tccgcgtcta
     2161 cgaagacggc ctcgcgtttc gctaccgttt accgcaactc gccaagccca ccgcgcgcgt
     2221 cgtcggcgaa cattccgggt ttcgcttcgc t
//
LOCUS       DASPLT010000540         2512 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_30163, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000540 DASPLT010000000
VERSION     DASPLT010000540.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 2512)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2512)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2512
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_30163"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1702)
                     /locus_tag="VEA63_05560"
     CDS             complement(<1..1702)
                     /locus_tag="VEA63_05560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HYD83493.1"
                     /translation="MLGLIAFANASSAGPKFRTSPAPSERLRQELWSAHWITHPAAPK
                     NEYGIYRFRKQIELTERPAQFVAHVSADARYRLFVNGESVCFGPQRSDKWVWHYETVD
                     LAPWLREGTNVIAAEVWSYGELTPYAIVGLRTSFLMQGDTEAERAVNTNASWKVSRDA
                     AFSPIEVKLPTYIVIGPGARVDAAAYPWGWHGEGFDDAAWSAVRVLARGNPYGRGTDM
                     DHWLKPRTIPLMEETPLRFAEVRRVSGAEVPADFVAGGAPFVIPAGGKATVLLDQGFE
                     TNAFPQLKVSGGKGGRVKLTYAEALIDNAGHKGNRNEVDGRRIAGVSDEFLPDGGDAR
                     VFSPLDFRTYRYVQIDVEAGAEPLRIDDLHGVFTAYPFHEHGAFESDDPQLAEIWTVG
                     WRTARLCAFETYVDCPYYEQLQYVGDTRIQALISLYVSGDDRLMRNAIELYDRSRIAE
                     GLTQSRYPSVTPQVINTFSLWWVEMVHDYWMHRKDDAFVSERLAGIEAVLGWFERRID
                     AKTGLLGPMDYWTFVDWTDEWRWDGPRGIGGEPPGARTGGSSVVSLQLAGTAMRAAEL
                     C"
     gene            complement(1904..>2512)
                     /locus_tag="VEA63_05565"
     CDS             complement(1904..>2512)
                     /locus_tag="VEA63_05565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD83494.1"
                     /translation="ALSVAQTWRERLGLARDAHWDDVIAKLAPLPQDDGKYVALESHP
                     DTWTNIASRHDHPEMLMPLGFLPEAPGVDRATMERTLDAVLTEWDWDTKIWGWDYPMI
                     AMTAARLGRPEVAVSVLLRDGPNNRYTANGHCPQRSDTALANGQSGPGRREIAVYLPG
                     NGSFLAAVGLMVGGWEGANGEQPGLPKDDSWKVKAEGWRPLP"
BASE COUNT          436 a          988 c          644 g          444 t
ORIGIN      
        1 ggcacagctc cgccgcgcgc atcgccgttc ccgccagctg caacgacacc acgctcgaac
       61 cgcccgttcg cgctcccggc ggctcgccgc cgatgccccg cggaccatcc cagcgccact
      121 catccgtcca gtcgacaaac gtccagtaat ccatcggtcc gagcaacccg gtcttcgcat
      181 cgatccggcg ctcgaaccag ccgagcaccg cttcgatccc cgccaaccgc tcgctcacaa
      241 acgcgtcgtc cttccgatgc atccagtagt cgtgcaccat ctcgacccac cagagcgaaa
      301 aggtgttgat cacctgcggc gtcaccgatg gataccggct ctgcgttaat ccttccgcga
      361 tccgcgaccg gtcgtagagc tcgatcgcat ttcgcatcag ccggtcgtcg cccgacacat
      421 acaatgagat cagcgcctgg atccgcgtgt cgcccacata ctgcagctgc tcgtaatagg
      481 ggcagtccac ataggtctcg aacgcgcaca gccgcgccgt ccgccagccc accgtccaaa
      541 tctcggccag ttgcggatcg tcgctctcga acgcgccgtg ctcgtgaaac ggatacgcgg
      601 tgaacacccc gtgcagatcg tcgatccgca acggctccgc ccccgcctcg acgtcgatct
      661 gcacgtagcg atacgtccgg aaatccagcg gcgaaaacac gcgtgcgtcg ccgccgtccg
      721 gcaggaactc gtcgctcacg cccgcgatcc tgcggccgtc gacttcgttg cgattgccct
      781 tgtgcccggc gttgtcgatc agcgcttccg catacgtcag cttcacccgc ccgccttttc
      841 cgccgctgac cttcaattgc ggaaacgcat tcgtctcgaa gccctggtcc aacagcaccg
      901 tcgctttccc gcccgccgga atcacgaacg gtgcgcctcc tgccacgaaa tccgccggca
      961 cctccgctcc gctcactcgc cgcacttcgg caaaacgcag cggcgtttcc tccatcaacg
     1021 gaatcgttcg cggcttcagc cagtgatcca tgtccgtgcc gcgcccgtaa ggattgcccc
     1081 gcgcgagcac gcgcaccgcg ctccacgcgg cgtcgtcaaa tccctcaccg tgccagcccc
     1141 acggatacgc cgccgcgtcc acccgcgccc ccggcccgat cacgatgtaa gtcggcagct
     1201 tcacctcgat cggcgagaac gccgcatcgc gcgaaacttt ccatgacgcg ttcgtgttga
     1261 ccgcccgctc cgcctccgtg tccccctgca tcagaaagct cgtgcgcaat cccacgatcg
     1321 catacggcgt cagctcgccg taactccaca cctccgcggc gatgacgttc gtcccttcgc
     1381 gcagccacgg cgcgagatcg acggtttcgt agtgccacac ccacttgtcg cttcgctgcg
     1441 gtccaaagca gaccgactcg ccgttcacga acaaccggta gcgcgcatcc gcactcacgt
     1501 gcgccacaaa ctgcgcgggt cgctccgtca gctcgatctg ttttcgaaat cgataaatcc
     1561 cgtattcgtt tttcggcgcc gccggatgcg tgatccaatg cgccgaccac agctcctgcc
     1621 gcaatcgctc cgatggcgcc ggcgacgtcc tgaacttcgg cccagccgac gacgcattgg
     1681 cgaacgcaat aagaccaagc atgaaaaaac agagacgcat gagtcgggaa tgtgcgtggc
     1741 acagtgagcc cccgcacccc tgtccgcaat gtccgacccg gcgaacccgt tcttcacccg
     1801 atgcaccgac cgcctgcttc gtggccctgc gcgtgagcgc agggattccg cggcgagagt
     1861 ccgcattgcc accatgcggc tccgcccgct tgttgccacc accttacggc agcggccgcc
     1921 agccctctgc cttcaccttc caactgtcat ctttcggcaa acccggttgc tcgccgttcg
     1981 cgccttccca gccgcccacc atcaatccca ccgccgctaa aaacgacccg ttccccggca
     2041 aatacaccgc gatctcccgg cgccccggac ccgattgtcc gttggccagc gccgtatcgc
     2101 ttctttgggg acaatgcccg ttcgccgtat aacggttgtt cggcccgtcg cgcagcagca
     2161 cgctcaccgc cacctccggc cggcccagcc gcgccgctgt cattgcgatc atcggataat
     2221 cccagcccca gattttcgtg tcccagtccc actccgtcag caccgcatcg agcgtgcgct
     2281 ccatcgtcgc gcgatccacc cccggcgctt ccggcaaaaa tcccaacggc atcagcatct
     2341 ccgggtgatc gtgccggctc gcgatattcg tccacgtatc cggatgcgat tccaacgcca
     2401 catattttcc gtcgtcctgc ggcagcggcg ccagcttcgc gatcacgtcg tcccaatgcg
     2461 catcgcgcgc gaggcctagc cgctcgcgcc acgtctgcgc cacgctcagc gc
//
LOCUS       DASPLT010000541         5752 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_171118, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000541 DASPLT010000000
VERSION     DASPLT010000541.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 5752)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5752)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5752
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_171118"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(37..366)
                     /locus_tag="VEA63_05570"
     CDS             complement(37..366)
                     /locus_tag="VEA63_05570"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HYD83495.1"
                     /translation="MQINESTHNGWTVFTLAGRIDHYSAEALKAALMPRMTGGAVALD
                     FTGVDYITSQGFRVLLIASKEQLAKKGRFVLGNMNEPLTAYFNMADLGTVFTIVQDIH
                     LAIDQAP"
     gene            complement(543..872)
                     /locus_tag="VEA63_05575"
     CDS             complement(543..872)
                     /locus_tag="VEA63_05575"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HYD83496.1"
                     /translation="MQINSSTQSGWTVLTLTGKIDNAGADELKAALAPHLTGGALALD
                     FAGVEYVTSSGFRVLLQAFKDLRAAGGQMILGNMSPALRGFFDMAGLSTVFKITHDIR
                     TVIDQAP"
     gene            complement(1032..1694)
                     /locus_tag="VEA63_05580"
     CDS             complement(1032..1694)
                     /locus_tag="VEA63_05580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007362366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070273 - phosphatidylinositol-4-phosphate
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GPP34 family phosphoprotein"
                     /protein_id="HYD83497.1"
                     /translation="MTTPHLTFVEELVLLALDDETGAQRPMPVMAFNYAIAGALLADL
                     ALANRVDTDPQQLVVLSREPLGDPLLDNALATIAAEPAPKPVAYWLSIFSEERPALEA
                     AALSRLVDRGILRREDKKVLWVFGVRRYPTVDNHERVEVRTRLAALILSDELPDPRDA
                     VLISLLTASHLAASMFAAPEFTARSQRIATLAKMDLVGREVAAAIEAVTYALRSALPL
                     GM"
     gene            complement(1846..2172)
                     /locus_tag="VEA63_05585"
     CDS             complement(1846..2172)
                     /locus_tag="VEA63_05585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007362367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HYD83498.1"
                     /translation="MDIIETQSNSATVLALGGRLDGLASPGLEQKVDALVASGARTIV
                     FDCSRLDYVSSAGLRAFLTSAKKLKAAGGKAAFAALAPTVREVFELSGFLNVLEVHST
                     VAAASA"
     gene            complement(2275..2697)
                     /locus_tag="VEA63_05590"
     CDS             complement(2275..2697)
                     /locus_tag="VEA63_05590"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007362368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HYD83499.1"
                     /translation="MSVTTLTLTLRNDLAEIPRLAAAVEAFCEPLEPTPKAAMAVQLA
                     LEEIVTNVINHGYTDGQPHTFTVDLTADDGRVILVVTDDASAYDPLARPDVDTSLPLE
                     QREIGGLGVHLVKKLMDRAHYERRDGHNILTLERSLRT"
     gene            complement(2857..3669)
                     /locus_tag="VEA63_05595"
     CDS             complement(2857..3669)
                     /locus_tag="VEA63_05595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007362370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HYD83500.1"
                     /translation="MLTISGIRKTFNPDTVNEVRALQGVDLTIDEGSFVIVLGMNGSG
                     KSTLLNAVAGTFFVDEGTLSLAGHDVTRWPEHKRAKLIGRVFQNPFSGTAPTMSIAEN
                     FALAARRGHARGLGWALVPGLMGPLRDRIASLKMGLENRLDNAIGSLSGGQRQALTLL
                     MATWLKPKLLLLDEHTAALDPKSADQVITLSDEVIQRDKLTTLMVTHSMQQAVTLGDR
                     IIMMHRGRVLHDFRGAEKKRLRTEDLLDRFDEVRRREQLDESAATMLNELYV"
     gene            complement(3787..4716)
                     /locus_tag="VEA63_05600"
     CDS             complement(3787..4716)
                     /locus_tag="VEA63_05600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007362371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HYD83501.1"
                     /translation="MTLLIGALTIGLILSLLAMGVFVSFRIFSMPDITTDGSITFGAA
                     IAAILLVNHVNPVLATAAGALGGALAGAVTGVIHTKFKINALLAGILVMTALYSVNLH
                     VMGRSNIPLMQSRTLVSAGEAFGETLFGTRTLDIFGWQVGSADAAVLLLSFLFAAFSG
                     AVLFAFFRTNLGTAMRATGDNPQMIRALGANVELHIILGLALSNGLVALSGALLAQYQ
                     GFADAQMGIGMVVWGLASVIIGEALTGTRSLGLTIVGALMGSVLFRLLVAIALRWGLN
                     PNDLKLITALFVFAALVLPPFIAKMKRKKLADA"
     gene            complement(4877..>5752)
                     /locus_tag="VEA63_05605"
     CDS             complement(4877..>5752)
                     /locus_tag="VEA63_05605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009514048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor antagonist"
                     /protein_id="HYD83502.1"
                     /translation="GGVQLNLIGNAAALTQAPATAARPPRMDFGAASFALGIGALGAN
                     YADCEPRFGEFLAIGGTAAFQPSDGSSRPDFVVSQGAFVPEGYLLLGLSGTGTFSTLA
                     RFEAKKEARTVGLAELAQTALDLSGAPAAAFIAITETAGLVGATLRQSPVAANGAAAP
                     SPRFGFPQIRDWLSFTSERAFRDSTSLIVGVAARPGTPLDPLLRPLQRPASSASQPST
                     LNPQPLLAHIHAASFPYRPLRKGRIELKPSLTELFDGHALQAVLHLLSDPREFNGAGE
                     SEFFRGALWIAPVTS"
BASE COUNT         1054 a         1375 c         2304 g         1019 t
ORIGIN      
        1 aatcagcggc tggagggcgc gggttcggcg tggacgtcag ggagcctggt cgatcgcgag
       61 gtgaatgtct tgaacgatgg tgaacactgt gccgaggtcg gccatgttga aataggcggt
      121 gagcggttcg ttcatgttgc cgaggacgaa gcggcccttt ttcgcgagtt gttctttcga
      181 tgcgatgagc agcacgcgaa agccctggct cgtgatataa tcgacgccgg tgaaatcgag
      241 cgcgacggcg ccgccggtca tccgcggcat caaggcagcc ttcaacgctt cggcgctgta
      301 gtgatcgatg cgtcccgcca gcgtgaagac cgtccagccg ttgtgggtcg attcattgat
      361 ttgcatggaa gaagcgcggc gggccggtga gggagaagcg gcgacgctcg aagaggacga
      421 cgagattaag ggccgcgcgg cggatggcca tccggtattt gcggaaacgc tgcgccggta
      481 ggggacggcc gggcgttggg cggcgggcga tctgtcgggg cgggacgagc ccgcgctcgc
      541 ggttatggcg cctgatcgat cacggtgcgg atgtcgtgcg tgattttgaa aacggtgctg
      601 agaccggcca tgtcgaagaa gccgcggagc gcggggctca tgttgccaag gatcatctgc
      661 ccgccggccg cgcggaggtc tttgaacgct tgcaacagaa cgcgaaagcc ggagctggtg
      721 acgtattcga cgccggcgaa atcgagggcc agcgcgccgc cggtgagatg gggcgcgagc
      781 gcggctttga gttcgtcggc accggcgttg tcaattttgc ccgtgagcgt gagaacggtc
      841 cagccgctct gcgtggagga gttgatctgc atggaggagg gagcgttaag cgtgcggcga
      901 gagaaggaga ggaaaaggcg tggcgcaacg gcgggcgggc gcggtggtgt tacaatcgcg
      961 tggcggtgac agcggcgccg gccggagcgg atgaagctgc aaacggagca ggcaggcgcg
     1021 gaggcccgcc gctacatgcc gagggggaga gcgctgcgga gcgcgtaggt gacggcttcg
     1081 atggcggcgg cgacttcgcg gccgacgagg tccattttcg ccagggtggc gatgcgctgt
     1141 gaacgggcag tgaactcagg cgcggcgaac atcgaggcgg cgagatggct cgcggtgagc
     1201 aggctgatca ggacggcatc gcgcgggtcg ggaagctcgt cgctcaggat gagcgcggcg
     1261 aggcgcgtgc ggacttcgac gcgttcgtgg ttgtcgaccg ttgggtagcg acggacgccg
     1321 aagacccaga gaactttttt gtcttcgcgg cgaagaatgc cgcggtcgac gaggcggctg
     1381 agcgcggcgg cttcgagcgc ggggcgttcc tcggaaaaga tcgagagcca gtaggcgacg
     1441 ggtttcggcg cgggctcggc ggcgatggtc gcgagggcgt tgtcgaggag cggatcgccg
     1501 agcggttcgc gactgaggac gacgagttgc tgagggtcgg tgtcgacgcg attggcgagc
     1561 gcgaggtcgg cgaggagcgc gccggcgatg gcgtagttga acgccatgac gggcatcggg
     1621 cgttgcgcgc cggtttcgtc gtcgagcgcg aggaggacga gttcttcgac gaaggtaaga
     1681 tggggagtgg tcatggcgaa ggaggattgg ggggcgtgct gaatgggcct gatctttctc
     1741 tttcttcttt ctctttatct ttctcctgct gattgcgggc tgcgagtttc ggacggtcgc
     1801 gctgacgaaa gagaaagata aagaggaaag agaaagaggc gagggtcagg cggaggcggc
     1861 ggcgacggtg gagtgaactt cgaggacgtt gaggaagccg gagagctcga agacttcgcg
     1921 gacggtggga gcgagggcgg cgaaggcggc tttgcccccc gcggctttca gttttttcgc
     1981 ggaggtgaga aacgcgcgaa gtcccgcgct gctgacgtag tcgaggcgcg agcaatcgaa
     2041 cacgatggtg cgcgcgccgg aggcgacgag cgcatcgact ttttgttcga ggcccgggct
     2101 ggcgaggccg tcgaggcgac cgccgagggc gagaacggtg gcggagttgg attgggtttc
     2161 gatgatatcc atggcggaaa ttgattttgg gtttgttcgg agttcggagt tccgggttgt
     2221 tgggaattgg gggagctaca gtaactcgga acgcggaaca cggaactctg aactttacgt
     2281 gcggagtgac cgttcgaggg tgaggatgtt gtggccgtcg cggcgttcgt aatgcgcacg
     2341 atccatcagc tttttcacga ggtgaacgcc gaggccgccg atctcacgtt gttcgagcgg
     2401 cagggaggtg tcgacatcgg ggcgcgcgag tgggtcgtag gcggaagcgt cgtcggtgac
     2461 gaccaagatc actcgtccgt cgtcggcggt gaggtcgacg gtgaacgtgt gcggttggcc
     2521 gtcggtatag ccgtggttga tgacgttggt gacgatttcc tcgagcgcga gctggacggc
     2581 catcgcggct ttgggcgtgg gctcgagcgg ttcgcagaac gcttcgacgg cggcggcgag
     2641 gcgggggatt tccgcgaggt cgttgcgcag ggtgagcgtg agcgtcgtga cggacatctt
     2701 tttgtttaac cgctaatctg cgctaatttt tcggagtcga gggaggtggc gggacgttgc
     2761 aggcttcgcc tgatcggcga agcacgggcg cgtgaacaga aagtgggtgg ctcggatagc
     2821 gatgattgat gcggattagt ggttaacgag atttttttag acgtagagct cgttgagcat
     2881 cgtcgcggcg gactcgtcga gttgttcgcg gcggcggact tcgtcgaaac gatcgaggag
     2941 gtcttcggtg cggaggcgtt ttttttcggc gccgcggaaa tcgtggagga cgcggccgcg
     3001 gtgcatcatg atgatgcgat cgccgagcgt gacggcttgc tgcatggaat gcgtgaccat
     3061 gagcgtggtg agtttgtcgc gctggatgac ttcgtcgctg agtgtgatga cttggtcggc
     3121 gcttttcggg tcgagggcgg cggtgtgttc gtcgaggagc aggagtttcg gtttcagcca
     3181 cgtggccatg aggagcgtga gcgcctggcg ttgtccgccg gagagcgagc cgatggcgtt
     3241 atcgaggcgg ttttcgagac ccatttttag cgaggcgatg cggtcgcgga gcgggcccat
     3301 gaggccgggg acgagcgccc agccgagtcc gcgcgcgtgg ccgcggcggg cggcgagggc
     3361 gaagttctcg gcgatggaca tcgtgggggc ggtgccgctg aacggatttt ggaaaacgcg
     3421 gccgatgagt tttgcgcgtt tgtgttcggg ccagcgggtg acgtcgtggc cggcgaggga
     3481 gagggtgcct tcgtcgacga agaaggtgcc ggcaacggcg ttgaggagcg tggatttgcc
     3541 ggagccgttc atcccgagaa cgatcacgaa ggagccttcg tcgatcgtga ggtcgacgcc
     3601 ttggagggcg cggacttcgt tgaccgtatc gggattgaag gttttgcgga tgccggagat
     3661 cgtgagcatg tgggcgggcg cgtgggaaga agagcgatcg ttctcgttct cttaatcgtt
     3721 ctcgttctcg aaactggacg gacgagagaa cgagaaagag aacgattacg agaacgagga
     3781 gggcgtttag gcgtcggcga gtttcttgcg tttcattttc gcgatgaaag gcgggaggac
     3841 gagcgcggcg aagacgaaga gcgcggtgat gagtttgaga tcgttcgggt tgagccccca
     3901 gcggagggcg atcgcgacga ggaggcggaa gaggacggag cccatcagcg cgccgacgat
     3961 cgtgaggcca aggctgcgcg tgccggtgag cgcttcgccg atgatgacac tggcgaggcc
     4021 ccagacgacc atgccgatgc ccatctgggc gtcggcgaag ccttggtatt gcgcgagcag
     4081 ggcgccggag agagcgacga gtccgttgga gagcgcgagg ccgaggatga tgtgcagctc
     4141 gacgttggcg ccgagcgcgc ggatcatttg cgggttgtcg ccggtggcgc gcatggcggt
     4201 gccgagattg gtgcggaaga acgcgaacaa cacggcgccg gaaaacgcag cgaagagaaa
     4261 cgagaggaga agaacggcgg cgtcggcgga gcccacttgc cagccaaaaa tgtcgagtgt
     4321 gcgcgtgccg aagagggttt caccgaaagc ttcgccggcg gagacgaggg tgcgggattg
     4381 catcaacggg atgttgctgc ggcccatgac gtgcaggttg accgagtaga gcgcggtcat
     4441 cacgaggatg ccggcgagga gggcgttgat cttgaacttg gtgtggatca cgccggtaac
     4501 ggcacccgcg agtgcgccgc cgagggcgcc ggcggcggtg gcaaggacgg ggttgacgtg
     4561 gttgacgagc aggatcgcgg cgatcgcggc gccgaaggtg attgagccgt cggtcgtgat
     4621 gtcgggcatt gaaaaaatgc ggaacgaaac gaagacaccc atcgcgagaa gcgagaggat
     4681 gaggccgatg gtgagggcgc cgatgagcag ggtcatggga ggcgaagcgg agaaaaggga
     4741 cggacggcga aagggagggc ggagttctgg ttcgttctcg ttctcttgat cgttctcgtt
     4801 ctcgaagcgt ttttagggag gagagaaaga gaacgattaa gagaacgaga acgaggcgga
     4861 gggtgagcgg gagtttttag gaagtcacgg gagcgatcca gagtgcgccg cggaagaatt
     4921 cgctttcgcc ggcgccgttg aattcgcggg ggtcagagag gaggtgcaac acggcttgca
     4981 gcgcgtggcc gtcgaagagc tcggtgagcg acggttttag ttcgatgcga cctttgcgca
     5041 gcggacgata cgggaatgag gcggcgtgaa tgtgggccag caacggttga gggttgagag
     5101 ttgaaggttg agaggcggaa gaggcgggac gctgcagcgg gcgaaggagc ggatcgaggg
     5161 gggtgcccgg gcgggcggcg acgccgacga tcaaggacgt cgaatcgcga aaggcgcgtt
     5221 cgctcgtgaa agaaagccag tcgcggattt gcgggaagcc gaagcgcggc gagggcgcgg
     5281 cggctccgtt cgcagcgacc ggcgattggc gaagtgtggc gccgacgagg ccggcggttt
     5341 cggtgatcgc gatgaacgcg gcggcgggcg ccccgctgag atcgagggcg gtttgcgcga
     5401 gttcggcgag gccgacggtg cgcgcctctt ttttcgcctc gaaacgcgcg agcgtggaaa
     5461 acgtgccggt gccggaaagg ccgaggagca gataaccttc gggaacgaaa gcgccctggc
     5521 tgacgacgaa atcgggacgg ctggagccgt cggacggttg aaacgcggcc gtgccaccga
     5581 tcgcgagaaa ttcgccgaag cgcggttcgc aatcggcgta gttggcgccg agcgcaccga
     5641 tgccgagtgc gaacgacgcg gcgccgaaat ccatccgcgg cgggcgggcg gcggtggcgg
     5701 gggcttgcgt gagcgcggcg gcgttgccga tgagattgag ctgcacgccg cc
//
LOCUS       DASPLT010000542         2105 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_30167, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000542 DASPLT010000000
VERSION     DASPLT010000542.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 2105)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2105)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2105
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_30167"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(197..742)
                     /locus_tag="VEA63_05610"
     CDS             complement(197..742)
                     /locus_tag="VEA63_05610"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HYD83503.1"
                     /translation="MPSRAAPPPKFDFSVIRRLREQHQMTLADLSEVSKVSTAVISKL
                     ERNQTTAELDTLFRLARAFGLSTTDLLALTESALAHQVDETTYRSGKFAFRQIRFANI
                     VGLLGEAPAGATIKRPEIHHDDTEVCWIISGKLRLTLPHEAFELDEGTSIQFDAILEH
                     SYEALSDVRLIILHLRKDKRY"
     gene            1110..1907
                     /locus_tag="VEA63_05615"
     CDS             1110..1907
                     /locus_tag="VEA63_05615"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HYD83504.1"
                     /translation="MSYSRGFSTLGCPELSLDEAFVLARSHRLDCVELRALAGTIELP
                     AYFKEKYVTPAAFAERLRASDVRVAALDTSLRLIGGSDADREALLRFVPWAEAARIAR
                     LRVFDGGKTGDETELAEAAAAMAWWRELRAQNGWRVDLMVETHDSLFTAAAIRRFLAL
                     APGAGILWDTHHTWKRGGEDPLVTWRAIRDAVVHVHVKDSVSKPSEKHPFTYVLPGDG
                     EFPMAPLRAAFAEGFSGAVSVEWERMWHPYLAPLDDVLRAAGERAWW"
BASE COUNT          403 a          470 c          828 g          404 t
ORIGIN      
        1 gttgagagtg agaggcgggc ggagcgcggt aggtgagagc ggaggggctt agagctgaga
       61 ggctagagct gagagcagga gctgcagagg gagtgagagt ctgtaattga gggtgaaaag
      121 ggtcatagcg gtggatgcag aaggatccgc tggatcgggg ctctagactc tcagctctaa
      181 gctctcagct tataagctag tagcgtttgt ctttgcggag gtggagaatg atgaggcgga
      241 cgtcggagag cgcttcgtag gaatgctcga ggatggcgtc gaactggatg ctcgtgcctt
      301 cgtcgagttc gaaggcttcg tgcggcagcg tgaggcgaag tttgcccgag atgatccagc
      361 agacctcggt gtcgtcgtga tgaatctcgg ggcgtttgat ggttgcgccc gcgggggctt
      421 ccccgagcag accgacgatg ttggcgaaac gaatctggcg gaaggcgaac ttgccggagc
      481 gataggtggt ttcgtcgact tgatgggcga gggcggattc agtgagtgcg agaaggtcgg
      541 tggtgctgag gccgaacgca cgagcgaggc gaaacagcgt gtcgagctcg gcggtggttt
      601 gattgcgctc gagtttggaa atcaccgcgg tcgagacctt cgacacctcg gaaaggtcgg
      661 cgagcgtcat ctggtgttgc tcgcgcaagc ggcggatgac gctgaagtcg aactttggcg
      721 gcggggcggc gcgggaaggc atgtttcgcg aaggcacaaa aaaccgtggt ttttatctgt
      781 caaggaaagt cggcacgaga ggcggctcag aagggatttg aaccacgaat ggacacgaat
      841 taacacgaat gaagaaaaag tgtgatgctg cgcggtgggc gggaatcgga tgacaacgga
      901 tgaacacgga tctgaaagat gaggggcggc gtgagggggc tgcgcgggtg gagaagaaag
      961 cggagcgggg gccaagagca ggaggtgtgt tatctgtgtt tatccgtgct tcagcggatg
     1021 gttcagtgag gagcggaggg cgccggtgaa ggggcggtga aaatttggag cgtcgacaag
     1081 ggggcggttg ttttcgcagc gtggagggca tgtcgtattc acgcggattt tccacgctgg
     1141 gttgtcctga gttgtcgctc gatgaggcgt tcgtgctggc gcggtcgcat cgactcgact
     1201 gtgtcgagct gcgcgcgttg gcggggacga tcgagttacc ggcgtatttt aaggagaaat
     1261 acgtgacgcc ggcggcgttc gcggagcggc tgcgggcgag cgacgtgcgt gttgccgctt
     1321 tggatacgtc gttgaggctg atcggcggaa gcgacgcgga ccgggaagcg ttgctgcgct
     1381 tcgtgccgtg ggcggaggcg gcgaggattg cgcggctaag ggtgttcgat ggcggaaaga
     1441 cgggcgacga aacggaactc gcggaggcgg cggcagcgat ggcgtggtgg cgcgagctac
     1501 gcgcgcagaa tggctggcgc gtggacttga tggtggagac gcacgacagc ttgttcacgg
     1561 cggcagcgat caggcggttt ctcgcgcttg cgccgggcgc ggggattttg tgggacacgc
     1621 atcacacttg gaaacgtggc ggcgaggatc cgctggtgac gtggcgcgcg attcgagatg
     1681 ccgtcgtgca cgtgcacgtg aaggacagcg tgagcaagcc gagcgaaaaa catccgttca
     1741 cgtatgtgct gccgggcgac ggtgagtttc cgatggcgcc gctgcgggca gcgtttgccg
     1801 agggattttc cggcgcggtg agcgtcgaat gggagcggat gtggcatcct tatcttgcgc
     1861 cgttggacga cgtgttgcgc gcggcgggcg agcgggcgtg gtggtgagtt gcgggatggc
     1921 cggcggcgag tgtgaggaac catgcaattg aaccacggat gaacaccggt agcgaaacgt
     1981 gtgagcgttt cgtttctcgc ccggacgaaa cgctcacgcg tttcgctaca acagactgcg
     2041 cgcacccgca tctgcctgtg atgaacgcgg gttggctgac cgctaacacc ggctcgagcc
     2101 cgtcg
//
LOCUS       DASPLT010000543         1717 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_46080, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000543 DASPLT010000000
VERSION     DASPLT010000543.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1717)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1717)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1717
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_46080"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..343)
                     /locus_tag="VEA63_05620"
     CDS             complement(<1..343)
                     /locus_tag="VEA63_05620"
                     /inference="COORDINATES: protein motif:HMM:NF012885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA lyase"
                     /protein_id="HYD83505.1"
                     /translation="MTRPAKIKIVEVGPRDGLQNESQIVSAAVKAELVHRLADAGLRT
                     IEVTAFVSPQRVPQMADAAEVMRMIVRRAEASYAVLVPNLRGFEAAMAAGAREIAVFG
                     SASETFSQRNIN"
     gene            complement(354..>1717)
                     /locus_tag="VEA63_05625"
     CDS             complement(354..>1717)
                     /locus_tag="VEA63_05625"
                     /inference="COORDINATES: protein motif:HMM:NF014805.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HYD83506.1"
                     /translation="EIQVFADAHGNCVHLFERDCSVQRRHQKVLEEAPAPGLTEERRR
                     EMGAAAIAAARAVGYVGAGTVEFIVDPDGGFYFMEMNTRLQVEHPVTEMITGLDLVEW
                     QLRVAAGELLPLAQVDITRAGHAIEARIYAEDPARDFLPSTGKLGYLSIPEESRHVRV
                     DTGVCAGDTITPHYDPMIAKLIVWDESREQALARMAAALARYRISGVTCNVEFLGRLV
                     TCPAFATGDLDTGLIARESVRLFPERAAVTRDACFAATLAVLAKEVQAVERSGERSSP
                     WVAADGWRLNSVQERTLHFRDGEAERLVQVRYEKTGYRLTLEGSGTVASGMLQGNAAL
                     RFTFDGVSGEATVVEEGDRLDVFLHGRKYELVRARAWNGGGETAAGDAGLTAPIPGHV
                     VALLAEPGAFVEQGAPLLVLEAMKMEMTIVAPTAGTVRAFRFPVGAQVAAGAELVEFE
                     KRG"
BASE COUNT          299 a          683 c          449 g          286 t
ORIGIN      
        1 agttgatgtt ccgctgcgaa aacgtttccg acgccgaccc gaagaccgcg atctcccgcg
       61 cccctgcggc catcgccgct tcgaacccac gcaggttcgg caccagcacc gcataacttg
      121 cctccgcgcg ccgcacgatc attcgcatca cctcggcggc atccgccatc tgcggcacgc
      181 gctgcggcga cacaaacgcg gtcacttcga tcgtccgcag ccccgcgtcc gccagtcgat
      241 gcacgagttc cgccttcacc gccgccgaca cgatctggct ctcgttctgc aagccgtcgc
      301 gcgggccgac ctccacaatc ttgatcttcg ccgggcgcgt catggtcgtt cgttcagccg
      361 cgtttctcga actccaccag ttccgcgccc gcggcaacct gcgcgcctac aggaaaacga
      421 aatgcccgca ccgtccccgc ggtcggcgcc acgatggtca tctccatctt catcgcctcg
      481 agcaccagca acggcgcgcc ttgctccacg aacgcaccgg gctctgccag cagcgcgaca
      541 acatggccgg gaatcggcgc cgtgagccct gcgtcgcccg cggccgtttc accgccgccg
      601 ttccacgccc gcgcgcgcac aagctcgtat ttccgtccat ggagaaacac gtccaaacga
      661 tcgccttctt cgacgaccgt cgcttcaccg ctcacgccat cgaacgtaaa ccgcagcgcc
      721 gcgttccctt gcagcattcc gctcgcaact gttccactcc cttcaagcgt cagccgatag
      781 cccgttttct catagcgcac ctgcaccaac cgctctgctt cgccatcgcg aaaatgcagc
      841 gtgcgttcct gcaccgaatt caaccgccag ccgtccgccg ccacccacgg cgagctgcgc
      901 tcgccggacc gctcgacagc ttgcacctcc ttcgccaaca cggccaacgt cgccgcgaaa
      961 cacgcgtccc gcgtgaccgc cgcgcgctcc ggaaaaagcc gcaccgactc gcgtgcgatc
     1021 aatcccgtat ccagatcgcc cgtcgcaaac gccgggcacg tcaccagccg tccgagaaac
     1081 tccacgttgc atgtcacgcc cgaaatccga tatcgcgcga gcgccgccgc catgcgggcg
     1141 agcgcctgct cccggctttc gtcccacacg atcagcttcg cgatcatcgg atcgtaatgc
     1201 ggcgtgatcg tgtcgccagc gcagacccct gtatccacgc ggacatgacg cgattcctcc
     1261 ggaatcgaaa gatagccgag cttcccggtc gagggcagaa agtcccgtgc gggatcctcc
     1321 gcatagatac gcgcctcgat cgcgtgcccg gcccgcgtga tgtccacctg cgccagcggc
     1381 agcagttcac cggccgcaac ccgcagctgc cactccacga ggtcgagccc ggtgatcatc
     1441 tccgtcaccg gatgctccac ctggagccgc gtgttcatct ccatgaagta aaatccgccg
     1501 tccggatcga cgatgaactc caccgtgccc gctccgacgt agccgaccgc tcgcgccgcc
     1561 gcgatcgccg ccgcccccat ttcccggcgt cgctcctctg tcagcccggg cgccggcgct
     1621 tcctcgagga ccttctggtg acgtcgctgc actgagcaat cgcgctcgaa aagatgcacg
     1681 cagttcccgt gcgcgtcggc aaacacctga atctcga
//
LOCUS       DASPLT010000544         1267 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_211570, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000544 DASPLT010000000
VERSION     DASPLT010000544.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1267)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1267)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1267
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_211570"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1253)
                     /locus_tag="VEA63_05630"
     CDS             complement(<1..1253)
                     /locus_tag="VEA63_05630"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HYD83507.1"
                     /translation="MLEADLACRTIEDRTRLIREKFGEEGERELSLESEALRLIEDRD
                     GDGTADFSTVYATGFNTPLDGVASGVVVRRGEVWFTNMPSFWKFTGEEKAETREELSR
                     GYGVRFNFTGHDLHGPIFGPDGKIYFSAGDRGSHVPTKEGGVIDVPDTGAVFRCNPDG
                     TELELFAWGLRNPQSLLFTENGDLFTGDNDSDQGDEERLVHLVEGGDSGWRVGYQFAP
                     LGDAGPWNSEHMWHPRHEGQPAFMLAPICNVEDGPSGVAYYPGTGLNEKYAGRIFVTH
                     FKGSIARSGIFDYKLKPQGASYAVEDARPLLTSSLPTDVRFGPDGKLYYSDWAEGWPK
                     STRGRIYTIFDPKSATDPLVKETKVLLASDFTKKSAAELVTLLSHADWRVRLEAQFTL
                     AESGEGSVAHFAAVLQRAQSGLAGL"
BASE COUNT          235 a          437 c          352 g          243 t
ORIGIN      
        1 tgcaaacccg cgagtcccga ttgggctcgt tgcaggaccg cggcgaaatg cgcgacactg
       61 ccctccccgc tttccgcgag cgtgaactgc gcttccagtc gcacgcgcca gtcggcatga
      121 ctcagcagcg tcacaagctc cgccgcgctc ttcttggtga agtctgacgc cagcagcacc
      181 ttcgtctcct tcaccagcgg atcggtcgcg ctcttcggat cgaagatcgt atagatgcgt
      241 ccgcgcgtcg acttcggcca gccttccgcc caatccgagt agtagagttt tccgtcgggt
      301 ccgaagcgca cgtccgtcgg cagcgacgag gtcagcaacg gccgcgcgtc ctccacggca
      361 tatgaggctc cctgcggctt cagcttgtag tcaaagatgc cgctgcgcgc gatgctgccc
      421 ttgaaatgcg tgacgaagat ccggccggca tacttctcgt tgagccccgt tccgggataa
      481 taggccacgc cggacggccc gtcttcgacg ttgcagatcg gcgcgagcat gaacgccggc
      541 tgcccttcgt gacgcggatg ccacatgtgc tcgctgttcc acgggccggc gtcgcccagg
      601 ggcgcgaatt gataacccac gcgccaaccg ctatcgccgc cctcgacgag atgcaccagc
      661 cgctcctcgt cgccctggtc ggagtcgtta tcgccggtga aaagatcacc gttctcggtg
      721 aacaacaaac tctgcggatt gcgcaggccc caagcgaaaa gctcgagctc cgtgccatcc
      781 ggattgcagc gaaacacggc gccggtatcc ggcacatcga tcacgccacc ttccttcgtc
      841 ggcacatgtg aaccgcgatc gcccgcgctg aaatagattt ttccgtccgg tccgaagatc
      901 gggccgtgaa gatcgtgacc ggtgaaattg aaacgcacgc cgtagccgcg actcagctcc
      961 tcacgcgtct cggctttttc ctcgcccgtg aatttccaga agctgggcat gttggtaaac
     1021 cagacctcgc cccgccgcac caccacgccc gatgcgacgc cgtcgagcgg cgtgttgaaa
     1081 ccagtcgcat acactgtcga aaaatctgcc gtgccgtcgc catcgcgatc ctcgatcaaa
     1141 cgcagcgctt cgctttcgag cgacaactcg cgttcgcctt cctcgccgaa tttttcgcga
     1201 atcaatcgcg tgcgatcctc gatcgtccgg cacgccagat cggcctcgag catccagaga
     1261 taattgc
//
LOCUS       DASPLT010000545         1954 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_30214, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000545 DASPLT010000000
VERSION     DASPLT010000545.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1954)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1954)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1954
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_30214"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(388..1242)
                     /locus_tag="VEA63_05635"
     CDS             complement(388..1242)
                     /locus_tag="VEA63_05635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007360075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HYD83508.1"
                     /translation="MPNLFIVAGPNGAGKSTYVKRFLPEEMRCREFVNADLIAAGLSP
                     FSPETAAFEAGRIMLRRLRELAYRREDFSFETTLSGRAYAPLLRELRSAGYRVRLDFL
                     WIPNLDITRNRVRQRVTKGGHNIPDAVQERRFHLGIRNLAELYRPLVDYWRLYDNTGD
                     SPHLVADEENGRLRIVDATRLAFIERNATVRFMPDQPAHTAEEAATFTPAEETRRSLR
                     AMRKAFADVVLENKAYGLPVIQWRDDRGVVEVPAEQLEPFARRILEVDGDPLPEEEER
                     ALLAHVKI"
     gene            complement(1393..>1954)
                     /locus_tag="VEA63_05640"
     CDS             complement(1393..>1954)
                     /locus_tag="VEA63_05640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007361176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycine dehydrogenase
                     (aminomethyl-transferring)"
                     /protein_id="HYD83509.1"
                     /translation="TEATKFAILKANYVAKRLDKFFPVLYRGHAGLVAHECIVDLRAW
                     KKFGVEVEDAAKRLMDYGYHAPTMSFPVPGTFMIEPTESEAKSELDRFCDALIAIHGE
                     MQAVATGESDKANNPLKHAPHTASVVCAAEWNHPYSRELAAFPSPSTRASKFWPSVGR
                     VDNVYGDRNLVCSCVGMEAYADSASE"
BASE COUNT          365 a          543 c          667 g          379 t
ORIGIN      
        1 gcacgatttt ttcggagaag tgcacccggg tgtttgagca tgtgtatgag acgcgggcgt
       61 cgtgatatac cctttccgtc cgccggactg cgtcgcgcga atggcgagga gaggcaaagg
      121 aaaaacgtgt cggcgcattc ccgctgcgca cgcggcgagt cacgaaaagc gcacgagcct
      181 gaccattacg agggcagagt tcttgaaccg ctaatctacg ctgatttcag aggccggcgg
      241 cgcgggcgaa agagtgcgtg cttcgcctaa tcggcgaagc gcggaagtca tcgggatccg
      301 cccagatcca ttagcggaga tgagcgatga ttagcggtta actgtattcg cgttccggat
      361 atgtcgtcgg gctggagcgc aaccccgtta gatcttcacg tgtgcgagga gggcgcgttc
      421 ttcttcttcg ggcaaggggt cgccgtcgac ttcgaggatg cggcgcgcga acggctcgag
      481 ctgctcggcc gggacctcga ccacgccacg atcatcgcgc cattgaatga cggggagacc
      541 gtaggctttg ttttcgagca cgacgtcggc gaaggctttc cgcatcgcgc ggagcgagcg
      601 tcgtgtttcc tcggcaggcg tgaacgtggc ggcttcctca gcggtgtgcg cgggctggtc
      661 gggcatgaag cgaacggtgg cgtttcgctc gatgaacgca agtcgtgttg catctacaat
      721 ccgtaggcgg ccgttttctt catcggcgac cagatgcgga ctgtctcccg tattgtcgta
      781 gagccgccaa taatcgacga gcgggcggta aagctcggcc aggttgcgaa tgccgagatg
      841 aaagcggcgt tcctgaacgg cgtcggggat attgtgcccg cctttggtca cacgctggcg
      901 aactcgattg cgagtgatgt cgaggttcgg gatccagaga aaatccaaac ggacgcggta
      961 gccggcgctg cgaagctcgc gcagcagcgg ggcgtaggcg cgaccggaca gtgtggtctc
     1021 gaacgaaaaa tcttcgcggc gataggcgag ctcgcgaagt cgtcgaagca tgatacggcc
     1081 ggcttcgaac gcggcggtct ccggagaaaa tggggagaga cccgccgcaa tcagatcggc
     1141 attcacgaat tcccggcagc gcatttcctc cggaaggaat cgcttcacgt aggtggattt
     1201 gccggcgccg ttcgggccgg cgacgatgaa caggttgggc acgacggatg agaacgtcgc
     1261 tgtgcgcgca gggcaacgca cgtttgttgc gatgggcggc gcgagatccc tggactcacg
     1321 cgcagagtcg cggacgggtc ggagacccgt ccctgccggc gctgccgcgc caatcgcttt
     1381 gttcgcgacg cattactcgc tcgcgctgtc ggcgtaggct tccatgccga cgcaggagca
     1441 gacgagattg cgatcaccgt aaacgttgtc gacgcggccg acgctcggcc aaaacttgct
     1501 cgcgcgcgtg gaaggcgaag gaaacgcggc gagctcgcgc gagtagggat gattccactc
     1561 ggcggcacag accacgctcg ccgtgtgcgg tgcgtgcttc agcgggttgt tcgctttatc
     1621 ggattcgccg gtcgcgacgg cctgcatctc gccgtgaatc gcgatgagcg catcgcagaa
     1681 gcggtcgagt tcgctcttcg cttcgctttc ggtcggctcg atcatgaacg tgccgggcac
     1741 gggaaacgac atcgtcggcg cgtgataacc gtagtccatg agccgtttcg cggcgtcttc
     1801 gacttcgacg ccgaattttt tccacgcgcg aagatcgacg atgcactcgt gcgcgaccaa
     1861 gccggcgtga ccgcgataaa ggacggggaa gaatttatcg aggcgctttg cgacgtagtt
     1921 cgctttgagg atggcgaact tggtggcttc ggtg
//
LOCUS       DASPLT010000546         1896 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_66776, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000546 DASPLT010000000
VERSION     DASPLT010000546.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1896)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1896)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1896
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_66776"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..602
                     /locus_tag="VEA63_05645"
     CDS             <1..602
                     /locus_tag="VEA63_05645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
                     /protein_id="HYD83510.1"
                     /translation="ALAKADWPTRFASALAAARDGATVGQLSRFSRGETAATEVIAPV
                     MTRRASADFEALRAASAGFAHRTGVRPGVFLAKMGPPLQHKARADFSAGFFAVGGFES
                     VAKQTFTTAEEAAAAAAASKAPVVVLCSTDETYPALVPAFAAGVRAASPDATIVLAGL
                     PADAAMVAAFRAAGIDEFIHLRANVHDVLAGLLKKMGAL"
     gene            602..>1896
                     /locus_tag="VEA63_05650"
     CDS             602..>1896
                     /locus_tag="VEA63_05650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HYD83511.1"
                     /translation="MKDVPDFTKLAYDAFSFGNAKPAAVADATSSQTYEQIPVKPVFT
                     ADDLPPQGELDTMPGLAPFTRGPYATMYVGKPWTVRQYAGFSTAEESNAFYKRNLAAG
                     QAGLSVAFDLATHRGYDSDHPRVVGDVGKAGVAIDSVLDMQTLFGGIPLDKVSVSMTM
                     NGAVLPVMAFYIGAALEQGAKLSDLSGTIQNDILKEFMVRNTYIYPPTPSMKIIADIF
                     AYTSTKMPKFNSISISGYHMQEAGATADLELAYTLADGLEYLKTGVAAGLDVDAFAPR
                     LSFFWAVGKNFFMEVAKMRAARTLWSEIVGRFNPKNAKSRALRTHSQTSGWSLTEQDP
                     FNNVARTCLEALAAACGHTQSLHTNALDEAIALPTDFSARIARNTQLHLQQETGVCNV
                     VDPWGGSYYVEYLTRELTAKARDHLAEVERLGGMTKAIEA"
BASE COUNT          331 a          567 c          671 g          327 t
ORIGIN      
        1 cagcgctcgc gaaagcggac tggccgacgc ggttcgcgtc ggcgctcgcg gcggcgcgcg
       61 acggcgcgac ggttggacag ctgagccgat tctcgcgcgg cgaaacagcg gcgacggaag
      121 tgatcgcacc cgtaatgacc cggcgcgcga gcgccgactt cgaggcgctg cgcgcggcat
      181 cagcggggtt cgcacaccgc accggggtgc ggccgggggt gtttctcgcg aagatgggac
      241 cgccgttgca gcacaaggcg cgggcggatt tttccgccgg gttcttcgcg gtcggcggtt
      301 ttgagagcgt cgcgaaacag accttcacga cggcggaaga agcggcggcg gcggcggcgg
      361 catcgaaggc gccggtcgtg gtgctgtgtt cgacggacga gacgtatccg gccttggtgc
      421 cggcgttcgc cgccggcgtg cgcgcggctt cgccggatgc gacgatcgtg ctggcgggat
      481 tgccggccga tgctgcgatg gtcgcggcgt tccgcgccgc ggggatcgac gaattcattc
      541 acctccgcgc caacgtccac gacgtgctgg ccggcctgct taaaaagatg ggagccctct
      601 gatgaaagac gtgcctgatt ttaccaagct cgcgtatgac gcgttctcgt ttgggaacgc
      661 gaaaccggcc gcggtggcgg acgctacctc ttcgcaaact tacgagcaga ttccggtgaa
      721 gccggtgttc accgccgacg atttgccgcc gcaaggcgag ctcgacacga tgccggggct
      781 cgcgccgttt acacgcgggc cttatgcgac gatgtatgtc ggcaagccgt ggacggtgcg
      841 ccaatacgcg ggattctcga cggcggagga gtcgaacgcg ttttataaac gaaatctcgc
      901 ggcgggtcag gcgggcttgt cggtcgcgtt cgatctcgcg acgcaccgcg gctacgacag
      961 cgatcacccg cgcgtggtgg gagacgttgg caaggcgggc gtggcgatcg acagcgtgct
     1021 cgacatgcag acgctcttcg gcgggatccc gctggataaa gtttcggtgt cgatgacgat
     1081 gaacggcgcg gtgctgcccg tgatggcgtt ctacatcggc gcggcgctcg agcagggcgc
     1141 gaagctgagc gatctttccg ggacgattca gaacgacatc ctgaaggagt tcatggtgcg
     1201 gaacacctac atctatccgc ccacaccctc catgaagatc atcgcggaca tcttcgctta
     1261 cacgtcgacg aagatgccga agttcaactc gatctcgatc tcgggttatc acatgcagga
     1321 agcgggcgcg acggccgacc tcgagctggc ttacacgctg gcggacgggc tggagtattt
     1381 gaaaacgggc gtcgcggccg ggctcgacgt cgacgcgttt gcgccgcggc tgtcgttctt
     1441 ctgggcggtc gggaagaact tcttcatgga agtcgcgaag atgcgcgccg cccgcacgct
     1501 gtggtcggag atcgtggggc ggttcaatcc gaagaacgcg aaatcgcgcg cgctgcgcac
     1561 gcattcgcag acctcggggt ggtcgctgac ggagcaggat ccgttcaaca acgtcgcgcg
     1621 cacgtgtctc gaagcgctgg ccgcggcgtg cggtcacacg cagtcgttgc acacgaacgc
     1681 gctcgacgaa gcgatcgcgt tgccgacgga tttctcggcg cgcatcgcgc ggaacacgca
     1741 gcttcatctc cagcaggaaa cgggcgtgtg caatgtagtc gatccgtggg gcggcagtta
     1801 ctacgtggaa tatctcacgc gcgaactcac cgcgaaggcg cgcgaccatc tggcggaagt
     1861 cgagcggctc ggcggcatga cgaaggcgat cgaggc
//
LOCUS       DASPLT010000547         1562 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_88868, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000547 DASPLT010000000
VERSION     DASPLT010000547.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1562)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1562)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1562
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_88868"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1237)
                     /gene="xseA"
                     /locus_tag="VEA63_05655"
     CDS             complement(<1..1237)
                     /gene="xseA"
                     /locus_tag="VEA63_05655"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HYD83512.1"
                     /translation="MSARDESLFAETEDRVESVSDFTRRVKTLLERGLRPGWVRGEVS
                     NLRAQASGHVYFCLKDSAAAMNAVLFRGDAARQSVKLRDGQQIVVYGEVSVYEARGTY
                     QFIVRAIIEDGEGRLRREFEALKQRLSDEGLFAAEKKRPLPEMPATIGFVTSPTGAAV
                     QDFLRILKRRGWRGRVVVLPAKVQGEGAAAEMAAMLELAQRLGIFDLLVIGRGGGSLE
                     DLWAFNEEPLVRAVAACAVPIMSAVGHEIDFSLCDFAADVRAETPSAAAEVISSHFVD
                     AAQRVLTASEGILTAGEDALERASARLDHARSRLRLLSPTAQVEQGWLRLDDLANRLA
                     GALRENVQRQRQRLEAARALLAHRSPEKRVQVESHRLLALWKRLQGASPQSVLNRGFV
                     IIRDEQGQPVARKNAVQRGQ"
     gene            complement(1253..>1562)
                     /locus_tag="VEA63_05660"
     CDS             complement(1253..>1562)
                     /locus_tag="VEA63_05660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD83513.1"
                     /translation="GDHTDLAIVVRVTPGIPFFVQNYLLGLAGVPLGKYLLVSCIISW
                     SYTVGFVVFGDALLHGKGKMALLAVSLLVAAAVITHWLRKHYARKKAHRKPPTVHENL
                     "
BASE COUNT          283 a          598 c          409 g          272 t
ORIGIN      
        1 tttgtccccg ctgcaccgcg ttcttccgtg ccaccggctg tccttgttca tcgcggatga
       61 tcacgaaacc ccggttcaat accgactgcg ggctcgcgcc ttgcaagcgc ttccacaacg
      121 caagcaaccg gtgcgactcg acctgcacgc gtttctccgg cgaacgatgc gcgagcaacg
      181 cccgcgccgc ctccaaccgc tgtcgctgcc tttgcacatt ctcgcgcaat gctcccgcca
      241 gccggtttgc cagatcgtcc aaacgcaacc acccctgctc cacctgcgct gtcggcgaga
      301 gcagccgcag tcgcgatcgc gcatgatcca gccgggccga cgcccgctcc agcgcgtcct
      361 cgccggccgt caggatccct tcgctggcag tcagcacgcg ttgcgcggca tcgacaaaat
      421 ggctcgagat cacctctgcc gccgcgctcg gcgtctccgc ccgcacatcc gccgcaaaat
      481 cgcacagcga aaaatcgatt tcatgcccca ccgccgacat gatcggcact gcgcaggccg
      541 ccaccgcgcg cacgagcggc tcttcattga acgcccacag atcctccaaa ctcccgccgc
      601 ctcggccgat caccagcaga tcgaaaatcc ccagtcgctg cgcgagctcg agcatcgccg
      661 ccatctccgc cgccgcccct tcgccctgca ctttcgcggg caacaccacg acgcgcccac
      721 gccagccgcg acgcttcaga atccgcaaga aatcctgcac cgccgcgccc gtgggcgacg
      781 tcacaaaacc gatcgtcgct ggcatctccg gcagcgggcg tttcttctcc gccgcaaaca
      841 gcccctcatc cgacaatcgt tgtttcagcg cctcgaactc ccgccgcagt cgtccttcgc
      901 cgtcctcgat gatcgcgcga acgatgaatt gatacgtccc gcgcgcttca tacacgctca
      961 cctcgccgta aaccacgatc tgctgaccgt cgcgcagctt cacggattga cgtgccgcgt
     1021 cgccgcggaa gagcaccgcg ttcatcgcgg cggcggaatc cttcaaacag aaatacacgt
     1081 gcccgctcgc ctgtgctcga agattggata cttccccgcg cacccagccc ggccgcaggc
     1141 cgcgctcgag cagcgttttc acccgacgcg tgaaatcgct gacggattcc acgcggtcct
     1201 ccgtctccgc gaacaggctt tcgtcccggg cgctcattcg gaatttcctc tgctacagat
     1261 tctcgtgcac cgttggtggc tttcggtgcg ccttctttcg cgcgtaatgt ttccgcagcc
     1321 aatgtgtgat caccgccgcg gccacgagca ggctcaccgc caggagcgcc atcttgccct
     1381 tcccatggag cagcgcgtcg ccaaacacca cgaaacccac cgtgtagctc cacgaaatga
     1441 tgcaggacac gagcaggtat ttacccagtg gcacgcccgc gaggccgagc agatagttct
     1501 gcacgaagaa cgggatgccc ggcgtcacgc ggaccacgat ggcgagatcg gtgtgatcgc
     1561 ct
//
LOCUS       DASPLT010000548         2245 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_149861, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000548 DASPLT010000000
VERSION     DASPLT010000548.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 2245)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2245)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2245
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_149861"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1127)
                     /locus_tag="VEA63_05665"
     CDS             complement(<1..1127)
                     /locus_tag="VEA63_05665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HYD83514.1"
                     /translation="MRYKILTVDDSKTVRIIVKKAFKSYDCEILEAANGVEGLALAAK
                     ETPDLILLDVTMPVMDGVEMLTKLKSDPALKGIPVMMLTAEGGRDNVLKIAKIGVRDY
                     LVKPFKEDVLIEKAGRVIDLKPLSETPAKAKSIFDPADILVVEDKPAIIQQIQEGLKH
                     TPWKIHGVSTQGEAIDFCSRTPPDMMLVSLSLPDESAFTLFRLIRTNVKTKYTPVFAL
                     VVKTETAQQQQAQTVGFSAIITKPIDLGELESKMAKAMNLDTSQRYFAIEGDSLIMRL
                     PENCSPAVIAEVSTYLKPKFAEAVDAGHNKVVIDIHQIRSLHMGIIKLLFQAMQTCRE
                     LGMQFALVANPQIVTECKGFEDTRNWTFYDSLEDAKANLGKS"
     gene            complement(1139..1684)
                     /locus_tag="VEA63_05670"
     CDS             complement(1139..1684)
                     /locus_tag="VEA63_05670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheX"
                     /protein_id="HYD83515.1"
                     /translation="MPAAAQITAPLIRENIARAVSDVFKTMLAREPVLTPVAGGDLDA
                     VWPAQSPLADNPRPHIVGTVGFIGDANGLIYLYLDLAFARHCTCHLLGMTEEELTEAG
                     DEVMNDAIGELTNMTVGSFKNGLCDAGYPCKLTIPSILHGSNFTIEPISSVTRHVYHF
                     DCSGHRIIADILMKQDDEPLS"
     gene            complement(1955..>2245)
                     /locus_tag="VEA63_05675"
     CDS             complement(1955..>2245)
                     /locus_tag="VEA63_05675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HYD83516.1"
                     /translation="APPLSVEESAPSSDVTPPPSAVAASGSTSVTDGQGQVLRLDALE
                     KQAIRAALRQTNGNRTQAAAELGISIRTLRNKLQEYRESGDPIDADMASIDG"
BASE COUNT          392 a          653 c          723 g          477 t
ORIGIN      
        1 gacttgccaa ggttcgcttt cgcgtcttcc agcgaatcgt agaacgtcca gttccgagtg
       61 tcttcgaatc ctttgcactc ggtgacgatc tgcgggttcg cgaccagcgc gaattgcatc
      121 ccgagttcgc ggcaggtctg catcgcctgg aataggagct tgatgatgcc catgtgcagc
      181 gagcggatct ggtggatgtc gatcacgact ttgttgtgac cggcgtcgac cgcctcggcg
      241 aacttcggct tcaaatacgt ggacacttcg gcgatgaccg ccggcgagca gttctcgggc
      301 aggcgcatga tgagcgagtc gccctcgatc gcgaagtaac gttgcgacgt gtcgagattc
      361 atcgccttcg ccatcttcga ctcgagctct ccgagatcga tcggcttcgt gatgatcgcg
      421 ctgaaaccca cggtctgcgc ctgctgctgc tgcgcggtct cggtcttcac gacgagcgcg
      481 aacaccggcg tgtatttcgt tttcacgttc gtgcggatca aacggaagag cgtgaacgcg
      541 gattcgtcgg gcaacgaaag actgacgagc atcatgtccg ggggcgtgcg tgagcagaaa
      601 tcgatcgctt cgccctgcgt gctcacaccg tggattttcc aaggcgtgtg cttcaggcct
      661 tcttggatct gctggatgat cgcgggcttg tcctcgacga cgaggatgtc ggccggatcg
      721 aaaatggact tcgctttcgc gggcgtttcg gagagcggct tgaggtcgat cacgcgcccg
      781 gccttctcga tcagcacgtc ttccttgaag ggcttaacga gatagtcgcg cacgccgatc
      841 ttcgcgatct tgaggacgtt gtcgcggccg ccttccgcgg tgagcatcat cacggggatg
      901 cccttgagcg cggggtcgga cttgagcttc gtcaacatct cgacgccgtc catgacgggc
      961 atcgtgacgt cgaggagaat gagatcgggg gtttccttcg cggcgagcgc gaggccctcg
     1021 acgccgttgg cggcttcgag gatttcacag tcgtaggact tgaaggcctt cttgacgatg
     1081 atgcggacgg tcttcgagtc gtccacggtg agaatcttgt agcgcatggt gtgtgtgatt
     1141 atgaaagggg ctcgtcgtcc tgcttcatga gaatgtcggc gatgatccga tgacccgagc
     1201 agtcgaaatg gtaaacgtgc cgcgtcacgg agctgatcgg ctcgatcgtg aaattgctgc
     1261 cgtgcaggat cgacgggatc gtgagcttgc agggatagcc cgcatcgcag aggccgttct
     1321 tgaaactgcc gaccgtcatg ttggtgagtt cgccgatggc gtcgttcatc acttcgtcgc
     1381 ccgcctcggt gagttcctcc tcggtcatgc cgagcaggtg gcaggtgcag tggcgcgcga
     1441 acgcgagatc gagataaagg tagatcaacc cgttcgcgtc gccgatgaag ccgacggtgc
     1501 cgacaatgtg cggccgggga ttgtccgcca gcggcgattg cgcgggccag actgcgtcga
     1561 gatcgccacc ggcgacaggc gtcaacacag gctcacgagc caacatggtc ttgaacacat
     1621 cgctaacggc gcgggcgatg ttttcgcgga taagaggagc ggtgatttgc gcggcggcag
     1681 gcatggcgtg gctggatgat gcggtgctct ggctgctatc ggcgcattcg tgaacggcct
     1741 taacaggatt ctcgccgctc gatgatttca gacaccgcga acctcgtccc actctgccct
     1801 gcccgcgcag tcggctcgct gacgaactgc gcgcaatcac gccgacagcc aatcgcggaa
     1861 ttgaaggtag gagcctccgt gcaggcgatt gagtggcgtg cgcgaggcga aatcgcctgc
     1921 aagcaggctc cacctattgc atcgcccgtc cggcttagcc gtcgatcgaa gccatgtccg
     1981 cgtcgatcgg atcgccggac tcccgatact cctgcaattt attgcgcagc gtgcgaatgc
     2041 tgatgccgag ctccgcggcg gcttgcgtgc ggttaccgtt ggtctgccgc agcgcagcgc
     2101 gaatcgcctg cttctccagc gcgtcgagtc gcaacacctg tccttgtccg tccgtgacgg
     2161 aagtcgaacc gctcgccgcg actgcactcg gcggcggcgt cacgtccgac gacggtgcgc
     2221 tttcttccac cgagagtggc ggcgc
//
LOCUS       DASPLT010000549         1556 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_108097, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000549 DASPLT010000000
VERSION     DASPLT010000549.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1556)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1556)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1556
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_108097"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            211..>1556
                     /locus_tag="VEA63_05680"
     CDS             211..>1556
                     /locus_tag="VEA63_05680"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF012630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031012 - extracellular matrix [Evidence
                     IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="matrixin family metalloprotease"
                     /protein_id="HYD83517.1"
                     /translation="MTRAPRLHTVAVSFVSILVGTALSLFAPLVMPAHVPIDDGIYYG
                     SHEALGISDDFGRFNSTIRLVYDPDGAPTAYSSNAHVLALLEEAFGYWTTVSGVQFEI
                     AGIDTNALDDRFVSNPDHRDGLVRVSWGAIGSVAGQAGPLSGWYDDSLGYFPFEDGTI
                     ELNNSAGVISSDAELIAVLVHEIGHLLGLGHSDNPDSAMYANPYNFLRYPREDDIRAI
                     QVLYGAPAVSIDVNSPVPQWIYTPPPQATSATTQFLFKPHANAASGAYFEVNDAPVTS
                     VTANTPNNQYVWFVGALGGGGTAIAIDATFVVVDPSGYIYDERPWELACSASSICLRS
                     ITLADGRIMKTIPGTWKIYVIDNATNQTLVSASLPVNTVPEYNRAPLATITAVPGSTP
                     ASARFTLTATDPEGDGIEVVWHPPNLPGAAIDVRQTIASGGSATQTIDFALTGTHT"
BASE COUNT          277 a          520 c          482 g          277 t
ORIGIN      
        1 gatggtacaa cggaacgggg gccgggaaaa attgcatcgg cgcgcgcgga aacacgggct
       61 gccggcactt tcgaaattcg tgcctcgggc gccctacgcg cggtgtagcc ggccggtcga
      121 aagccgctat actcggctcg ccctgacgct ccgccccgca gggcgcaagc ggccgactgg
      181 catcagtggc ctggtatcga ggaccactgc atgacccgcg cgccgcgcct gcacaccgtt
      241 gctgtttcgt tcgtttccat ccttgttggc accgccctca gtttgttcgc accgctggtg
      301 atgccggccc atgtgccgat cgatgacggc atctattacg gtagccacga agcgctcggc
      361 atcagcgacg acttcggccg tttcaattcc acgatccgac tcgtgtacga ccccgacggc
      421 gcgccgacgg cgtacagcag caacgcgcac gtgctcgcgc tgctcgaaga ggctttcggt
      481 tactggacca cggtcagcgg cgtgcagttt gagatcgccg gtatcgatac caacgcgctc
      541 gacgatcgct tcgtcagcaa tcccgatcat cgcgatggcc tcgtgcgcgt gtcgtggggt
      601 gcgattggca gtgttgcggg acaggctggt ccgctgagcg gctggtatga cgactcgctc
      661 ggctactttc cgttcgaaga cggcacgatc gagttgaaca acagcgccgg cgtgatttcg
      721 tccgacgccg agctgatcgc cgtgctcgtg cacgagatcg gccacctgct cggactcggg
      781 cattccgaca atcccgactc ggcgatgtac gcaaacccgt acaacttcct gcgctacccg
      841 cgtgaggacg acatccgcgc gatccaggtg ctttatggcg cgcccgccgt ctcaatcgac
      901 gtgaacagcc cggtgccgca gtggatttat acaccgccgc cgcaggccac ttcggccacg
      961 acgcagttcc tgttcaagcc gcatgcgaac gccgcgagcg gcgcgtactt cgaagtgaac
     1021 gacgcgccgg tgacgagcgt caccgccaac acgccgaaca atcagtacgt ctggttcgtc
     1081 ggcgcgcttg gcggcggcgg cacggcaatc gccatcgatg ccacgttcgt cgtcgtcgat
     1141 ccgagcggat acatctacga tgaacgtccg tgggagctgg cctgttccgc gagcagcatc
     1201 tgcctgcgtt cgatcacgct ggcggatggc cgcatcatga agacgatacc gggcacgtgg
     1261 aagatttacg tcatcgacaa cgccacgaac cagacgctcg tgtccgcgtc gctgcccgtc
     1321 aacacggtgc cggaatacaa ccgcgcgccg ctcgcgacga tcaccgcggt gcccggaagc
     1381 acgcccgcgt cggcacgttt cacgctcacg gccaccgatc cggaaggcga cggcatcgaa
     1441 gtggtctggc atccgccgaa tctgcccggc gcggcgattg acgtgcggca gaccatcgcg
     1501 tcgggtggat cggcgacaca gacgatcgat ttcgcgctca ctggcacgca cacctt
//
LOCUS       DASPLT010000550         2772 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_88901, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000550 DASPLT010000000
VERSION     DASPLT010000550.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 2772)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2772)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2772
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_88901"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..587)
                     /locus_tag="VEA63_05685"
     CDS             complement(<1..587)
                     /locus_tag="VEA63_05685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar nucleotide-binding protein"
                     /protein_id="HYD83518.1"
                     /translation="MKIFLTGASGLVGSAFARAAARNGHEIVGTVGRFRGKIAGLTRQ
                     LTLDLTDETAVTKALLDSEAEAVVNAAAISEPAACEKDPQGSAAMNVALPATLARLAQ
                     RLGAALVHVSSEQVFDGERSTAYAKGDATSPINVYGGQKVASERAVLEAASKLAAVVR
                     APLLMGDSPGGQRSLHERLLGDWAAGRTARLYVDE"
     gene            complement(653..2212)
                     /locus_tag="VEA63_05690"
     CDS             complement(653..2212)
                     /locus_tag="VEA63_05690"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HYD83519.1"
                     /translation="MKKPAAKKSAGLPPSLLAAALRAQADGVFVAEAKSTARGLKILF
                     VNESFCAMTGQAAAELVGRPHGALHVDKADVSKLTGWLRNAEPGKTLAGEGYLVRKDG
                     GTIYAAWSFSAVVAARGRVSHVVATYRDTTEKRRLQEALVHSQRLDAVGRLAGGVAHD
                     FNNLLSVINGYCEMLAAQVSDRPQALREVTEIHKSGLRAATLTRQLLAFGRRQPMDAH
                     IINFNRIVQDNAEILKRLVGTAGRLELDLARKVGNVRADPTQFQQVLLNLVINARDAL
                     RDRGCITISTSNRDVRPGLNRRVTDVPPGHYVCLTIADNGTGMDADTQKHLFEPFFTT
                     KPEGKGTGLGLALVYGIVQQSSGYISVRSALLAGSTFEILLPEVREPEEAPSPATPIS
                     PLPATRGSETLLIVEEDEVVRKMVAGMLTADGYRVLAAKNAAEGRKIAREQSSQSRPV
                     QLLIAGLADGGEALARLLHEQLRELRVLCIGNYDAPETLPWLDANRQASLAKPFALSE
                     ILRSVRALLDA"
     gene            complement(2209..>2772)
                     /gene="pdxH"
                     /locus_tag="VEA63_05695"
     CDS             complement(2209..>2772)
                     /gene="pdxH"
                     /locus_tag="VEA63_05695"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007360256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HYD83520.1"
                     /translation="RQFEKWFQEAEAAKLVEPNAMTVATASRDGRPSVRTILLKGVDG
                     RGFVFYTNFESRKGRELEANPRAALLFPWIALERQVIVEGPVTKVSREEAEAYFHSRP
                     LPSQLSAWVSQQSSIISGRKVLEDAMKEVEKKYAGQPVPLPPHWGGFRVAPETVEFWQ
                     GRRSRLHDRLRYRRGDDGGWIIDRLAP"
BASE COUNT          461 a         1004 c          803 g          504 t
ORIGIN      
        1 aactcgtcca catatagccg cgcggtgcgc cccgccgccc aatctccaag cagccgctcg
       61 tgcaagctcc gctgtccgcc ggggctgtcg cccatgagca gcggcgcacg caccaccgcg
      121 gcgagtttcg acgcggcttc gagcaccgcg cgctcgctcg ccactttctg cccgccataa
      181 acattgatcg gcgaagtcgc gtcgcccttc gcatacgccg tcgaacgttc accgtcgaac
      241 acctgttccg aagaaacatg caccagcgca gcgcccaagc gctgcgccag tcgggccagc
      301 gttgccggca gcgcgacgtt catcgccgct gatccctgcg ggtctttctc gcatgcggcc
      361 ggctccgaaa tcgccgccgc gttcacgacc gcctccgctt cggaatccag cagggccttc
      421 gtcaccgcgg tttcgtcggt gaggtcgagc gtgagctgac gcgtcaggcc ggcgattttc
      481 ccgcgaaaac ggccaacggt tccgacgatt tcatgaccgt ttcgcgccgc cgctcgcgcg
      541 aatgcggaac ccaccaatcc cgatgctccg gtgagaaaaa ttttcatggt tgatggaatc
      601 actctcgcgc gacccgcgct cgcgaatccc gacgcgtcca cacgtgctta cgtcacgcgt
      661 ccaacagcgc gcgcacgctg cgcaggattt cactcagcgc aaacggcttc gcgagcgaag
      721 cctgacgatt cgcatccagc cagggcagcg tttcgggcgc atcataattt ccgatacaca
      781 acacgcgtaa ttcgcgcagc tgctcgtgca gcaatcgcgc cagcgcctcg ccgccgtcgg
      841 ccagccccgc gatcagcaac tgcacgggcc ggctttgcga gctctgctcg cgcgcgatct
      901 ttcggccttc ggccgcgttc ttcgccgcga gcacgcggta gccgtcggcc gtcagcatgc
      961 ccgcgaccat cttgcgcacc acttcgtcct cttcgacaat tagcagggtt tcgctgccgc
     1021 gcgtcgcggg cagcggcgag atcggcgttg ccggcgaggg agcttcctcc ggttcccgca
     1081 cctcgggcag cagaatttcg aacgtcgatc ccgcaagcag tgcgctgcgc accgaaatgt
     1141 agccgctgct ctgctgcacg atgccataga cgagcgcgag ccccagcccc gtgcctttgc
     1201 cttccggctt tgtcgtgaag aatggctcga acaaatgctt ttgcgtgtcc gcgtccatcc
     1261 cggtgccatt gtccgcaatc gtcaggcaaa cgtagtgtcc cggcggcaca tccgtgacgc
     1321 gacgattcag tcccggccgc acgtcgcggt ttgaagtgct gatcgtgata cagccgcgat
     1381 cgcgcaacgc gtcgcgcgcg ttgatcacga ggttgagcaa cacttgctga aactgcgtgg
     1441 ggtcggcgcg cacgttgccc accttccgcg cgagatcgag ctcgagccgg cctgcggtgc
     1501 cgaccagccg cttcaggatc tcggcgttgt cctgcacgat ccggttgaaa ttgatgatgt
     1561 gcgcgtccat cggctgccgc cgtccaaacg ccagtagctg ccgcgtaagc gtggccgcgc
     1621 gcaggccgga cttgtgaatc tccgtcactt cgcgcaacgc ctgcgggcga tccgacacct
     1681 gcgccgcgag catctcgcag taaccgttga tcaccgaaag caggttgttg aaatcgtgcg
     1741 cgacaccgcc cgcgagccgc cccacggcgt cgagccgctg cgaatgcacc agcgcttcct
     1801 gcagccggcg cttttctgtc gtatcgcgat acgtggcgac cacgtgcgac acccgcccgc
     1861 gcgccgccac gacggcgctg aaactccacg cggcgtagat ggtgccgccg tccttgcgca
     1921 cgagatagcc ttcgccggcg agtgtctttc ccggttccgc gttgcgcagc cagcccgtga
     1981 gcttggaaac gtcggccttg tcgacgtgca acgcgccgtg cggccgcccc acgagttccg
     2041 cggctgcctg gcccgtcatc gcgcaaaaac tctcgttcac aaaaagaatc ttcagcccgc
     2101 gcgccgtcga tttcgcctcc gccacgaaca cgccgtccgc ctgcgcccgc aacgccgcgg
     2161 cgagcagtga aggaggcaat ccggccgact ttttcgcggc gggtttcttc acggagcgag
     2221 tcgatcgatg atccagccgc cgtcgtcgcc gcgccggtaa cgcagccgat cgtgcagccg
     2281 gctgcgccgc ccctgccaga attccaccgt ctccggcgcc acgcgaaaac caccccagtg
     2341 cggtggcagc ggcacgggct ggccggcgta ttttttttcc acttccttca tcgcgtcttc
     2401 gagcactttg cggcccgaga tgatcgagct ctgttgcgac acccaagccg acaactggct
     2461 cggcagcggc cggctgtgaa aatacgcctc cgcttcctcg cgcgacacct ttgtcaccgg
     2521 cccttcgacg atcacctgcc gctctagcgc aatccaggga aacagcagcg ccgcgcgcgg
     2581 gttcgcctcg agctcacgac ctttgcgact ctcgaagttc gtatagaaaa caaagccgcg
     2641 cccatcgact cccttcagca aaatcgtgcg cacggacggt cgtccatcgc gactcgccgt
     2701 cgcgacggtc atcgcgttcg gctccaccag cttcgccgct tccgcttcct gaaaccattt
     2761 ctcgaactgg cg
//
LOCUS       DASPLT010000551         2339 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_66817, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000551 DASPLT010000000
VERSION     DASPLT010000551.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 2339)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2339)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2339
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_66817"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..379
                     /locus_tag="VEA63_05700"
     CDS             <1..379
                     /locus_tag="VEA63_05700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD83521.1"
                     /translation="RGIPAGVASNDCVLIWWVDKAGYRLELAGLRVATAGRYRVLARF
                     VREHEMGRFAFDLNGVRLGGEFDLYAPTKGKPPLLDDVPVELGVVELAAGEHVFGAET
                     LEKNASSSGYLLCVDYFKLVPIS"
     gene            376..1776
                     /locus_tag="VEA63_05705"
     CDS             376..1776
                     /locus_tag="VEA63_05705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007360612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HYD83522.1"
                     /translation="MNDVNASAPENTAPTTDAAGRKIWTVGTLTYTTGGLVILFCWLL
                     AGDFAWSMKERSVMWVVQLLIKKFEASDMIAGLLIGTLPQALALILTPIVSYRSDRHR
                     GRWGRRIPYLIIPTPIAALSMVGLAFSPAIGTWLHGVLGETSPGLNPSILIVFGVFWT
                     VFEVATVTANAVFFGLINDVVPKAVLGRFYGMFRAMGLIVAMAFNYWLFGKAETHYTA
                     IFIGIGILYGVGFTTMCFKVKEGEYPPPAPIPPSRAGGLVAAVRTYFRECFTRPYFLW
                     IYASVSFAWMAMLTANLFNLFFAKSVGMSMDTYGKYLAATFLISFFMSYFIGALADRW
                     HPLRLSLGLLAIFAVVMVAGGLLVTDDRSFGVALIAYGVLGGSWQTAAASLTMRLFPQ
                     ARYAQIDSAKAIVQSFGMMLVGPAMGYFLDLSGHDYRHAYTASGIMALLSLLLGLVVH
                     AKFMKLGGPKGYVAPE"
     gene            2083..>2339
                     /locus_tag="VEA63_05710"
     CDS             2083..>2339
                     /locus_tag="VEA63_05710"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HYD83523.1"
                     /translation="MKRFFFGLFLSVSWVSGAAASENGTRPNFVVIIADDLGWGDLSS
                     YGHPRIETPSIDRLAADGIRFTSCYSTSPVCSPSRAGLLTGR"
BASE COUNT          394 a          564 c          830 g          551 t
ORIGIN      
        1 gcgcgggatt cccgccggtg tggcgagcaa cgattgcgtg ctgatctggt gggtggacaa
       61 ggcggggtat cggctggagc tcgcgggact tcgcgtggcg acggcggggc gttatcgcgt
      121 gctggcgcgg ttcgtgcgcg agcatgagat ggggcggttc gctttcgatt tgaacggcgt
      181 gcggttgggc ggggagttcg atctttacgc gccgacgaaa gggaagccgc ctctgctcga
      241 cgacgtgccg gtggaactcg gtgtcgtcga actcgcggcg ggcgagcatg tgttcggcgc
      301 ggagacgctg gagaagaacg cgtcgtcgtc ggggtattta ttgtgcgtcg actatttcaa
      361 actcgtgcca atttcatgaa tgacgtgaac gcttccgccc ccgaaaacac cgccccgacg
      421 accgatgccg cggggcgcaa gatctggacg gtgggcacgc tgacgtatac gacgggcggg
      481 ctggtgattt tgttttgctg gctgctggcg ggcgatttcg cgtggtcgat gaaggagcgg
      541 tcggtgatgt gggtggtgca gctcctgatc aaaaaattcg aggcgtcgga catgatcgcg
      601 ggtttgttga tcggcacgct gccgcaagcg ctcgcgctga tcttgacgcc gatcgtgagt
      661 tatcgctcgg accggcatcg cggacggtgg ggacggcgga ttccttattt gatcatcccg
      721 acgccgatcg cggcgctgtc gatggtgggg ctggcgttta gtccggcgat cggcacgtgg
      781 ttgcacggcg tgttgggaga aacgtcgccc ggattgaatc catcgattct gatcgtgttc
      841 ggcgttttct ggacggtgtt cgaggtggcg acagtgacgg cgaacgcggt gtttttcggg
      901 ttgatcaacg acgtggtgcc gaaggccgtg ctggggcggt tctacgggat gtttcgtgcg
      961 atgggactga tcgtggcaat ggcgttcaac tactggctgt ttggcaaagc ggagacgcac
     1021 tacacggcga tcttcatcgg catcggaatt ctgtatggcg tcggtttcac gacgatgtgt
     1081 ttcaaggtga aggagggcga gtatccgccg ccggcgccga ttccgccgag ccgggcgggc
     1141 ggtctggtgg cggcggtgag aacgtatttt cgggagtgtt tcacgcgacc gtatttcctg
     1201 tggatctacg cgtccgtctc gttcgcgtgg atggcgatgt tgaccgcgaa tttgttcaat
     1261 ctgttcttcg cgaaaagcgt cggcatgagc atggacacgt atgggaaata tttggcggcg
     1321 acgtttctga tttcgttttt catgtcgtat ttcatcggtg cgctcgcgga ccgttggcat
     1381 ccgttacggc tgagtcttgg gctgctggcc atctttgcgg tcgtgatggt ggcgggcgga
     1441 ttattggtca cggacgaccg gagtttcggc gtcgcgctga ttgcgtatgg cgtgttgggc
     1501 ggctcgtggc agacggcggc ggcgtcgttg acgatgcggt tgtttccgca ggcgaggtat
     1561 gcgcaaatcg actcggcgaa ggcaatcgtg caaagttttg gaatgatgct ggtcgggccg
     1621 gcgatgggct actttctcga cctaagcggg catgactatc gacacgcgta taccgcgagc
     1681 ggaatcatgg cgctgctgtc attgctcttg gggctggtgg tgcacgcgaa gttcatgaag
     1741 ctcggcggac cgaaggggta tgtggcgccg gaatgagcgg gaccgcgcgg gcggaagctc
     1801 caagagccaa gcaccaagct ccagtgaagc ttcaaggttc aattaccaga atgaagagcc
     1861 tgatttttaa ccacggatga acacggatgg acggcgatgg acgcggatgc ggatgaatgg
     1921 agtggttaca cagagctcac cgagctcgaa ccacagagat cacggagcgg atccgcgggc
     1981 attacttcat gaggtcaact cgcacctgcg tgatctatgt gcagtcggtt gattcgttgc
     2041 tctcaagcca agcggcttaa cttgctcccg atttttactc ctatgaaacg ttttttcttt
     2101 gggctttttc tttcggttag ttgggtgagt ggcgcggctg cgtcggagaa cggaacgcgg
     2161 ccgaatttcg tggtgatcat cgcggacgat ctcggatggg gcgatttgag ttcgtatggg
     2221 catccgcgaa tcgagacgcc gagcatcgat cggttggcgg cggacggaat tcgtttcacc
     2281 agttgttatt ccacctcgcc ggtgtgttcg ccgtcgcgcg cggggttgct gacgggacg
//
LOCUS       DASPLT010000552         1983 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_191610, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000552 DASPLT010000000
VERSION     DASPLT010000552.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1983)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1983)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1983
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_191610"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1646
                     /locus_tag="VEA63_05715"
     CDS             <1..1646
                     /locus_tag="VEA63_05715"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HYD83524.1"
                     /translation="RWWMELSDTGLDLRQSVLVFVDLTELKQAQAALAAEKERLAVTL
                     RAMAEGVITTDVDGVVQFMNPAAAAFTQIGSEMAIGQGVGEICLFENDRTGEPIELPV
                     RRVTQGDVVLELPPRTRLVVRSGASRLVEGCCAPIHSATSTVIGTVLVFRDVTEHDRL
                     EQELVRATKLESVGILAGGIAHDFNNILTAIMGNVALAALDVDPASDAGRSLNEAEKA
                     TLRARDLTQQLLTFAKGGEPVRAAVQLDGIVREMTTFTLHGSRVRADYDFAPDLWPAD
                     ADKGQIGRVVQNLVLNAVQAMPEGGTVKIALRNVQIDGLSRLALAPGNYVQIAIADTG
                     IGIKPEHLSRIFDPYFTTKQMGSGLGLAAVYSIVHKHRGTIDVDSQLGRGTTFNIWLP
                     ASFDAVMVNGGEKRGGAAVTLKGRLLFMDDEDSIRQMAVILLRRFGFEVACATDGAQA
                     VRMYQEAQDGGEPFTLVMMDLTVPGGMGGREAIAQLRRIDPKVRAIVSSGYSSDPVLA
                     NYREHGFCGVAAKPYEMSDLARVLREALAGDEADRAGAS"
     gene            complement(1694..>1983)
                     /locus_tag="VEA63_05720"
     CDS             complement(1694..>1983)
                     /locus_tag="VEA63_05720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007360843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD83525.1"
                     /translation="NPPTWFSSFRDLTLYCHIFLRHDVVIESEDPDPYFRWMRPRGGL
                     DFVEDFVRPGSEDGVHLDVEHNYPRSVITDRIAPENVHRLIAQINSCRGLS"
BASE COUNT          347 a          501 c          786 g          349 t
ORIGIN      
        1 tgcgctggtg gatggagttg agtgacacgg gactcgatct gcggcaatcg gtgctggtgt
       61 tcgtcgattt gacggagttg aagcaggcgc aggcggcgct ggcggcggag aaggagcggt
      121 tggcggtgac attgcgcgcg atggcggagg gcgtgatcac gacggacgtc gacggcgtgg
      181 tgcagttcat gaatccggcg gcggcggcgt tcacgcagat cgggagcgag atggcgatcg
      241 ggcagggcgt gggcgaaatt tgcctgttcg aaaacgatcg cacgggagaa ccgatcgaat
      301 tgccggtgcg ccgggtgacg cagggcgacg tggtgctcga gctgccgccg cgcacgcggc
      361 tggtggtgcg cagtggagcg agccggctgg tcgaagggtg ttgcgcgccg atccattcgg
      421 cgacgagcac ggtgattgga acggtgctgg tgtttcgcga cgtgacggag cacgaccggc
      481 tggaacagga actcgtgcgc gcgacaaagc tcgagtcggt gggtattctc gcgggcggca
      541 tcgcgcacga tttcaataac attctcactg cgatcatggg caacgtggcg ctggcggcgc
      601 tcgacgtgga cccggcttcg gatgccgggc gcagcttgaa cgaggcggaa aaagcgacgt
      661 tgcgcgcgcg cgatctcacg cagcagttgt tgacgtttgc gaagggcggc gagccggtgc
      721 gcgcggcggt gcaactggac gggatcgtgc gcgagatgac gacgttcacg ctccatgggt
      781 cgcgggtgcg cgcggactac gatttcgcgc cggatctctg gccggcggat gcggacaagg
      841 gccagatcgg ccgggtggtg cagaacctgg tgttaaacgc agtgcaggcg atgccggagg
      901 gtggcacggt gaaaattgcg ctgcgtaacg tgcagatcga tgggttgtcg cggctggcgc
      961 ttgcgccggg caattatgtg cagatcgcga tcgcggacac gggcatcggg atcaagccgg
     1021 agcacctttc gcggattttc gatccgtatt tcacgacgaa gcaaatgggc agcgggctgg
     1081 gactcgcggc ggtttactcg atcgtgcaca agcatcgcgg cacgatcgac gtcgactcgc
     1141 agcttggccg gggaacgacg ttcaacatct ggctgcccgc gtcattcgac gcggtgatgg
     1201 tgaacggcgg cgagaagcgc gggggtgcgg cggtgacgtt gaaggggcgg ctgttgttca
     1261 tggacgacga ggattcgatc cggcagatgg cggtgatttt gttgcgtcgt ttcggcttcg
     1321 aggtcgcgtg tgcgacggat ggcgcgcaag cggtgcgcat gtatcaggaa gcgcaggacg
     1381 gcggggagcc gttcacgctg gtgatgatgg acctgacggt gccgggcggg atgggcgggc
     1441 gggaagcgat cgcacagttg cggcggatcg atccgaaggt gcgtgcgatc gtgtcgagcg
     1501 gctactcgag cgatccggtg ctggcgaatt atcgcgagca cggcttctgc ggcgtggcgg
     1561 cgaaacctta cgaaatgtcg gatctcgcgc gcgtgttgcg cgaggcgctg gcgggcgacg
     1621 aggcggaccg cgcgggtgcg agttgatggg cggaaacgcg gcgggggcgc cgcggctcca
     1681 tggcggagcg gattcagctc aggccgcgac agctgttgat ctgcgcgatg aggcggtgaa
     1741 cgttttccgg cgcgatgcgg tcggtgatga cggagcgcgg gtagttgtgc tccacgtcga
     1801 gatgcacgcc gtcttcgctg ccgggtcgga cgaagtcctc gacaaagtcc agaccgccac
     1861 gaggtctcat ccagcgaaaa tacggatcgg gatcttcgga ctcgatgacg acatcgtgac
     1921 ggaggaaaat gtggcagtag agagtaaggt cgcggaagga gctgaaccac gtgggcgggt
     1981 tca
//
LOCUS       DASPLT010000553         1159 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_30263, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000553 DASPLT010000000
VERSION     DASPLT010000553.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1159)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1159)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1159
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_30263"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..304)
                     /locus_tag="VEA63_05725"
     CDS             complement(<1..304)
                     /locus_tag="VEA63_05725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HYD83526.1"
                     /translation="MNLNELPLEHALLTDRNIAIKLVGVGGAGSNAVDRLKMENLERL
                     QLAVVNTDYQALASSPVQDKVLIGMGVTRGLGAGGDPELGREAAEADREKIATVVKE"
     gene            complement(477..>1159)
                     /locus_tag="VEA63_05730"
     CDS             complement(477..>1159)
                     /locus_tag="VEA63_05730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cell division FtsA domain-containing protein"
                     /protein_id="HYD83527.1"
                     /translation="LEVRELVLSSLASGHMITTPEERQHGVLAIDIGAGTTDFALYRD
                     GVAHTTGVVPVGGTHLTNDLSIGLRLTEGQAEKLKLRFGRATVQARDKQEKVWLDGNF
                     AIGDRQFPRQAIEQITSARTWELLEVVRKKLGNAFSPETCAAGVVLTGGTAKLVGIAE
                     AAAKVFGVPAHLGETPAWVTENLRDPGYNTALGLLYYGVSAQNERLAPTRRSSGFLSN
                     VKRLFATA"
BASE COUNT          209 a          434 c          296 g          220 t
ORIGIN      
        1 actctttcac caccgtcgcg atcttctcgc gatccgcctc cgccgcctca cggccgagct
       61 ccggatcgcc gcccgccccc aacccgcgcg tcacgcccat gccgatcagc accttgtcct
      121 gcaccggcga gctcgcgagc gcctgatagt cggtgttcac caccgcgagc tgcaagcgct
      181 cgagattctc catcttgagc cggtcgaccg cgttcgaacc cgcgccgccg acgccgacga
      241 gtttgatcgc gatgttgcga tccgtgagca acgcatgctc gagaggaagt tcgttgagat
      301 tcatgtcaga aaatgagccg cccgcaaatc acgcgaatcg ccgcgtatta gctgggcaga
      361 aaacaaaatt atattcggtc aaaatcattc ccgccgcagg cacggggttc tctggttcat
      421 tcttcgcgac gattcgcgtc attcgcagga ggtgatcggt attcgcggct cgatcttcac
      481 gccgtcgcaa acaaccgctt cacgttggac aaaaacccgc tgctgcgccg cgtcggcgcc
      541 agccgctcgt tttgcgcgct cacgccataa tacagcagtc ccaacgccgt gttgtaacca
      601 ggatcgcgca aattctccgt cacccacgcc ggcgtttcgc cgagatgcgc cggcacgccg
      661 aacacctttg ccgccgcctc tgcgattccc accagcttcg ccgtgccacc ggtcaacacg
      721 acgcccgccg cacatgtctc cggcgaaaac gcattgccca acttcttccg cacgacctcc
      781 aacaattccc aggttcgcgc cgacgtgatc tgctcgatcg cctgccgcgg aaactgccgg
      841 tcgccgatcg cgaaattccc atcgagccac accttctcct gcttgtcccg cgcctgcacc
      901 gtcgcgcggc cgaagcgcag cttcagtttc tctgcctgcc cttccgtcag ccgcagcccg
      961 atgctgagat cgttcgtcaa atgcgtgccg cccaccggca ccacacccgt cgtgtgcgcc
     1021 acgccatcgc gatacagcgc gaaatccgtg gtccccgcgc cgatgtcgat cgccagcacg
     1081 ccatgctgcc gttcctccgg cgttgtgatc atgtgacccg acgcgaggct cgagagcacc
     1141 agctcgcgca cttccaaat
//
LOCUS       DASPLT010000554         1227 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_30280, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000554 DASPLT010000000
VERSION     DASPLT010000554.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1227)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1227)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1227
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_30280"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..625
                     /locus_tag="VEA63_05735"
     CDS             <1..625
                     /locus_tag="VEA63_05735"
                     /inference="COORDINATES: protein motif:HMM:NF017745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF885 family protein"
                     /protein_id="HYD83528.1"
                     /translation="GALEPEGETFFTVSPTPADWPEARKRSFYREYNDFMLHDLAVHE
                     AMPGHFLQIAHSNQFRAPTLVRAVFASGTFIEGWAVYAERVMADAGFGGPEVKMQQLK
                     MRLRAIINAILDNKVHTAGMTEQQALELMIRRGFQEEGEAVGKWRRACQSSTQLSTYF
                     VGAVEHDDMRAAAEKKEGAAFDLKRYHDRVLSRGSPAVKYVRNELGW"
     gene            684..>1227
                     /locus_tag="VEA63_05740"
     CDS             684..>1227
                     /locus_tag="VEA63_05740"
                     /inference="COORDINATES: protein motif:HMM:TIGR01643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD83529.1"
                     /translation="MATPRGWRNGQTVNTSTYATDLSLTGVAANYTYNAYGELTGSSH
                     RSLSWDYDLAGNRKFETDATGITNYTPDSLNQYASITGQRQEPGYDYDPDGNLKQDAI
                     WTYSYDAENRLIQMTKSGQTLTFKYDYLGRRIQKTVTGTGAYDIKFLWNGWTMIGELA
                     ANGTTANKTFVWGQDFSDAQG"
BASE COUNT          267 a          357 c          403 g          200 t
ORIGIN      
        1 gggagctctc gagcccgagg gcgagacgtt cttcacggta tcgcccacgc cggccgactg
       61 gccggaggcg cgtaagcggt cgttctatcg tgaatacaac gatttcatgt tgcacgactt
      121 ggcggtgcac gaggcgatgc cgggacattt cctgcagatc gcgcactcga atcagtttcg
      181 cgcgccgacg ctcgtgcgcg cggtgttcgc gagcgggacc tttatcgagg gttgggcggt
      241 gtatgcggag cgcgtgatgg ccgacgcggg atttggcgga cccgaggtga agatgcagca
      301 gctcaagatg cggctgcgtg cgatcatcaa cgcgatcctc gacaacaagg tgcacaccgc
      361 gggtatgacc gagcagcagg cgctggagct gatgattcgc cgcggttttc aggaggaagg
      421 cgaagccgtg ggcaaatggc gtcgcgcgtg ccagagctcg acgcagctct ccacgtattt
      481 cgttggagcg gtggagcacg acgacatgcg cgccgccgcg gagaagaagg aaggcgcggc
      541 ctttgatctg aaacgttacc acgaccgcgt gctctcgcgc ggcagccccg cagtgaaata
      601 cgtgcggaac gagctgggtt ggtgatcaga ggtagcgggt gatctccggg tgagcggaac
      661 agaccgggaa gatacctaaa agagtggcga cgccgcgcgg ctggcgcaat gggcaaacgg
      721 tcaacacctc gacgtatgcc accgacctga gcctcacggg cgtggccgcc aactacacct
      781 acaatgccta cggcgaactc acgggcagtt cgcaccgtag tctgagctgg gactacgatc
      841 tcgcgggcaa ccggaaattc gagaccgacg ccaccggcat cacgaactac acgcccgatt
      901 cgctcaacca atacgcctcg atcaccggcc agcgacagga gccgggatac gactacgatc
      961 cggatggaaa cttgaaacag gacgcgatct ggacgtattc ctacgatgcg gagaatcgcc
     1021 tcatccagat gaccaagtcg ggccagacac tcacgttcaa atacgactat ctcggtcgcc
     1081 ggattcagaa aacggtcacg ggcaccgggg cgtatgacat caagttcctg tggaacgggt
     1141 ggacgatgat cggcgagctc gctgccaacg gcacaaccgc gaacaagacg ttcgtctggg
     1201 gccaggactt ctccgacgcg cagggcg
//
LOCUS       DASPLT010000555         1453 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_231418, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000555 DASPLT010000000
VERSION     DASPLT010000555.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1453)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1453)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1453
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_231418"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1048
                     /locus_tag="VEA63_05745"
     CDS             <1..1048
                     /locus_tag="VEA63_05745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD83530.1"
                     /translation="AIRPGDRLKPVDNRDCIKIGGGAGHPVHHIVIDHVSCSWAVDET
                     ASTWSDKSTVSDVTFSACIFSEPVINGGHSKGSHPYGALGGRHSSNLSLIGNLFAFNF
                     GRNPLIRDETAGAQVVNNFIYRPGIWSNGTIYIGDLTLPPHAVSVVGNVIVRHPVPSQ
                     LEMTGTDGVRKLQEFQESDYRSVSIYVHDVVSPEAGLYLHDNRVFNPQTAVWHPVDGD
                     PWNPEIFRDSRKHPVARLAADPYANSGGTAWTPLPSSEVEARVLANAGKHPAQRDALD
                     AALIEKIRTRTGSFLQDLTGDDPWASVDVRNTRALSLPENPSADDDGDGYTNLEERLH
                     QLAAELERGGPPPR"
     gene            1116..>1453
                     /locus_tag="VEA63_05750"
     CDS             1116..>1453
                     /locus_tag="VEA63_05750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD83531.1"
                     /translation="MKRSFPSPVRFLSATILALLSSVLLRALPVVPGAVGFGTDTPAG
                     RGGIVYRVTNLNNSGPGSLRFGIEDVSMRQPRVIVFEVSGVIDLQSDLVIRDDSIGAY
                     SHLTIAGQTAP"
BASE COUNT          252 a          484 c          434 g          283 t
ORIGIN      
        1 cgcgatccgg ccgggcgacc ggctgaagcc ggtcgataac cgcgattgca tcaagatcgg
       61 cggcggcgcc ggtcatccgg tgcatcacat cgtgatcgac catgtttcct gcagttgggc
      121 ggtcgatgaa acggcgtcca cctggagcga caaaagcacg gtgagcgacg tgacgttttc
      181 cgcgtgtatt ttcagcgagc cggtgatcaa cggcgggcac agcaaaggct cgcatccgta
      241 tggggcgctc ggcgggcggc actcgtcgaa tctgtcgctg atcggaaatc tcttcgcttt
      301 caacttcggc cgtaatccgt tgatccgcga cgagaccgcg ggcgcgcagg tggtgaacaa
      361 tttcatttac cgtcccggca tctggtcgaa cggcacgatt tacatcggcg atctcacgct
      421 cccgccgcac gccgtctccg tggtgggaaa tgtcatcgtg cgccatccgg tgccgtctca
      481 gctcgagatg acgggcaccg acggcgtgcg gaagcttcag gagtttcagg agagcgatta
      541 tcgcagcgtt tccatttatg tgcacgacgt cgtgtcgccc gaagccggtc tctatctgca
      601 cgacaaccgc gtgttcaatc ctcagacggc tgtgtggcat ccggtcgacg gcgatccgtg
      661 gaatcccgag attttccgcg acagcagaaa gcatccggtc gcacgactcg ccgccgatcc
      721 atatgcgaac tccggtggga cggcgtggac tcccctgcct tcgagcgagg tggaagcgcg
      781 cgtgctggcg aacgccggga agcatccggc gcaacgcgac gcgctcgatg cggcgttgat
      841 cgaaaagatc cgcacgcgca ccggatcgtt tctccaggac ctgaccggcg acgatccgtg
      901 ggcgtcggtc gacgtgcgta acacgcgtgc gctctcgctg ccggagaatc cctccgcgga
      961 cgacgacggc gacggctata cgaatttgga agaacgcctg catcaactcg ccgctgaact
     1021 cgaacgaggc ggccctccac cgcgttagcg gcgccgtcct gcttttttaa atacgcaacc
     1081 cccactttca ccccgctcct cgccaccccc caatcatgaa acgctcgttt ccttcccccg
     1141 tccgattcct gagcgcgacg atcctcgcgt tgctcagttc agttctcctc cgcgcgcttc
     1201 cggtcgttcc cggcgccgtc ggcttcggca ccgatacgcc tgctggccgc ggcggcatcg
     1261 tttaccgcgt gaccaacctc aacaacagcg gccccggttc cctgcgcttc ggcatcgaag
     1321 acgtgagcat gcggcagccg cgtgtgatcg tgttcgaggt gtcgggcgtc atcgatctcc
     1381 agtccgatct cgtcattcgc gacgattcca ttggcgccta ctcgcacctg acgatcgccg
     1441 gccagacggc gcc
//
LOCUS       DASPLT010000556         1256 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_149914, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000556 DASPLT010000000
VERSION     DASPLT010000556.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1256)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1256)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1256
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_149914"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..578
                     /locus_tag="VEA63_05755"
     CDS             <1..578
                     /locus_tag="VEA63_05755"
                     /inference="COORDINATES: protein motif:HMM:NF019552.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="heparinase II/III family protein"
                     /protein_id="HYD83532.1"
                     /translation="GQPVLIDLGSARYEARTFGPQRYDIWINQSGWHNVPTIGDVMQS
                     AGREFKATDVLYRTDHDRAEISFELATAYPAEAQVNTWRRTVTLHRGSEIVIADAGEL
                     AKPQRVDFNFITPLAPDVSAGELTLRGEDGVDFRLRFDASLVEPHIEPVEVTDAWLRN
                     SWGAKIWRVVLRTRSEITRAKTVFHLSSASR"
     gene            709..>1256
                     /locus_tag="VEA63_05760"
     CDS             709..>1256
                     /locus_tag="VEA63_05760"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Plug domain-containing protein"
                     /protein_id="HYD83533.1"
                     /translation="MKLTLHRKAAIMMAICSTVVSQSQTTTPANAASQPLEEEIIELS
                     PFQVVTEKDRGYRATNSTSGTRLNAALKEIPMPIEVITAEFIRDIGASDLRQALSYSS
                     GIVLESQYDMGRDLTDITDAAEGITGAKEQTQIKLRGFATTETLRRGFRRASYSDSVG
                     IDRIEVVRGPAALLYGIGNFGG"
BASE COUNT          270 a          348 c          393 g          245 t
ORIGIN      
        1 acggccaacc tgtgttgatc gatctcggat cggcgcgcta cgaagcgcgc acctttggtc
       61 cgcagcgcta tgatatatgg ataaaccagt cgggctggca caacgtcccg accatcggcg
      121 atgtgatgca atcggccggc cgggaattca aggcgaccga tgtgttatat cgcacggacc
      181 atgatcgcgc ggagatttca ttcgagctgg ctacggcgta tccggcggaa gcccaggtga
      241 acacgtggcg gcgcacggtg acactccatc gcgggagcga gattgtgatt gcggacgccg
      301 gcgagctcgc gaaaccgcag cgggtggatt tcaatttcat cacgccgctg gcgccagacg
      361 tgagcgctgg ggaattgacg ctgcgtggag aggacggcgt tgattttcgg cttcgcttcg
      421 atgcgagttt ggttgagcca cacatcgagc ccgtcgaggt gacggatgcg tggttgcgca
      481 acagttgggg cgctaagatt tggcgcgtcg tgttacgcac gcggagcgaa atcacgcggg
      541 cgaaaaccgt ttttcacctg agttccgcga gtcgctgacg cggagattct ttccaagaaa
      601 acaacagaaa attccgtggt tatgccattc aggaagttcg gggggccggc tagactgaac
      661 gtgtaggtcg ggtgatttcc accgacctaa aaacctcacc ccaaaaagat gaaactgacc
      721 ttgcatcgaa aagcggcgat catgatggcg atatgttcga ccgtcgtgtc gcagtcgcaa
      781 accacgactc cagccaacgc ggcctcgcag ccgctcgaag aagaaatcat agaactctcc
      841 ccctttcagg tcgtgaccga gaaggaccgc ggttatcgcg cgaccaacag cacctcaggc
      901 acgcgtctca acgccgcgtt gaaagagatc cccatgccga tcgaggtgat cacggcggag
      961 ttcattcgcg atattggcgc gagcgatttg cgccaggcgc tgtcgtatag ctcgggtatc
     1021 gtgctcgaat cgcaatatga catgggccgc gatttgaccg acatcacgga tgccgccgaa
     1081 ggcatcacgg gggcgaagga gcagacgcag atcaagctgc gcggattcgc aacgaccgag
     1141 acgcttcgtc gcggctttcg ccgcgcgtcc tattcggaca gcgtcggcat cgatcgtatt
     1201 gaagtggtgc gcggtccggc cgcgttgctt tacggcatcg gcaatttcgg cggcat
//
LOCUS       DASPLT010000557         1034 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_66868, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000557 DASPLT010000000
VERSION     DASPLT010000557.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1034)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1034)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1034
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_66868"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..222)
                     /locus_tag="VEA63_05765"
     CDS             complement(<1..222)
                     /locus_tag="VEA63_05765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD83534.1"
                     /translation="MMSASQFRPIRLISLFAGVGIVPFLVGAEAAPWFKSGFESGVEL
                     RYDEPTRAMDTLEGRDATTGFDWSRDVPAA"
     gene            555..>1034
                     /locus_tag="VEA63_05770"
     CDS             555..>1034
                     /locus_tag="VEA63_05770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HYD83535.1"
                     /translation="MFLRTVETDLGQLPADVEWLDTAELTEASWHQQLVSRAPHVLLT
                     AWSAPPLPPAWLQTPGCSLRYVCHLTGSVRRLVPRFFLEQGGLVSNWGTLAAATVAEH
                     ALLLGLAALRNLPAWRPHLLRPPAERSVDDLATRSLFRRRVGLHGFGAIARELVPLLE
                     "
BASE COUNT          169 a          358 c          299 g          208 t
ORIGIN      
        1 cgccgccggc acatcgcgcg accagtcgaa accggtcgtc gcgtcgcgtc cctccagcgt
       61 atccatcgcg cgcgtgggtt cgtcgtagcg cagttccact ccggattcga aacctgattt
      121 aaaccacggc gccgcttcag ctcccaccag aaaggggacg atcccgacac cggcaaaaag
      181 agaaataagc cggatcgggc ggaattggct cgcgctcatc acacccgcat ttgatttatc
      241 ggaatcgtgt cgtcgcaacc aaattcgatt tgctcctcgc accccgcgct cgtttcacct
      301 ctccggtttg aagcaagccc cacgcccgta aaagatgtct ctaatgcgta tgtgatttat
      361 tggatgcaat ccctgcgagg caccggcgac gtcgcaaaag cggaatccga gatcgcggca
      421 ccgtcgtgtt tgcacgggct cgatcttgcc caagcgtaat aaacatggcc gcccaagaga
      481 ctgccgtttc gccttcggtc gcacctgcga cgcgaacacg catccttggc gttttgtcgg
      541 cggaagagcg ccgcatgttt cttcgcacgg tcgagaccga cctcggccag ttgcccgccg
      601 acgtggaatg gctcgacacc gccgaattga cggaagcctc ctggcaccag caactcgtta
      661 gtcgtgcgcc gcacgtcttg ctcacggcgt ggagcgctcc tcccctcccg ccggcctggc
      721 tgcagacgcc gggctgctct ctgcgctacg tctgccatct caccggctcg gtgcgccgcc
      781 tggtgccgcg tttttttctc gagcaaggcg gcctcgtgtc caactggggc acgctcgccg
      841 cggccaccgt cgcggaacat gcgctgctgc tcgggctcgc cgcgctgcgt aatcttcccg
      901 cctggcggcc gcatctgctg cgaccgcccg cagagcgttc cgtcgacgat cttgcgacgc
      961 gttcgctgtt tcgccggcgc gtcggcttgc atggattcgg tgcaatcgcg cgtgagctcg
     1021 tgccgctgct cgag
//
LOCUS       DASPLT010000558         1624 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_46192, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000558 DASPLT010000000
VERSION     DASPLT010000558.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1624)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1624)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1624
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_46192"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..234)
                     /locus_tag="VEA63_05775"
     CDS             complement(<1..234)
                     /locus_tag="VEA63_05775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012377330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HYD83536.1"
                     /translation="MHFDNLQPQLAAPLPRSDDDDDDDDEKENAPSKKGTQPVGMLIQ
                     KKFLDQRKIFLWGAVTDETAKDLTEKLLYLEAID"
     gene            complement(448..>1624)
                     /locus_tag="VEA63_05780"
     CDS             complement(448..>1624)
                     /locus_tag="VEA63_05780"
                     /inference="COORDINATES: protein motif:HMM:NF019260.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="nucleoside hydrolase-like domain-containing
                     protein"
                     /protein_id="HYD83537.1"
                     /translation="YAKVRDNLLLHEPGYPTAEHLLSVVSEGRPAYGMTAVGEAMDSP
                     GSTQLIASVDRDDSRPVWVLVWGGPNVLAQALWKVRATRSPEALDQFIGKLRVYAIAD
                     QDDSGPWIRKNFPDLFYVVSPGVHDGGAYHYATWNGISGDQFHGRFVGADFTLVDNPW
                     LDAHIRCKGPLGAEYPHVEYLMEGDSPSFLFVIENGLNAPEHPDWGGWGGRYERYTPP
                     MRKWFVEPETRPIWTDADDEVLGVDGHWHTSNQATIWRWRSAMQNDFATRMDWTIKPF
                     AEANHPPQPKLAHPPQLTARPGQRVELSAEGSTDPDGDALSYTWFYYPEPGTFTIADG
                     RSGAPLKIENSDQSTASFTVPTRGVLRTGTMHFILAVNDHGAPPLTRYQRVIVTVAP"
BASE COUNT          284 a          411 c          611 g          318 t
ORIGIN      
        1 atcgatggcc tcgagataga ggagcttctc ggtgaggtcc ttggcggttt cgtcggtgac
       61 cgcgccccag aggaagattt ttcgctgatc cagaaatttt ttctgaatca acatcccgac
      121 cggctgcgtg ccctttttgg agggagcgtt ttccttctcg tcgtcgtcat cgtcatcatc
      181 gtccgaacgc ggaagcgggg cggcgagctg gggctgaaga ttgtcgaaat gcataggggg
      241 ccaaactgat aagctccgga tgcttttggc aactgcggaa cggtgtttgc cgcggtagcg
      301 cggtcgacct gaccgcgaat tggcgaggtc cagacacggt cagttgaccg tgcgacgtgg
      361 ggcgcgtgtt cgccgatcca aagggacggc gggcgtttgg gaagcaggca ggcagggacg
      421 cctgccgcta ccttgtgtgg gggtgagtca gggagcgacg gtgacgatga cccgttggta
      481 acgagtgagc ggcggggcgc cgtggtcgtt gacggcgagg atgaaatgca tcgtgccggt
      541 gcggagcacg ccgcgcgtgg ggacggtgaa cgacgcggtg gattgatcgc tgttttcgat
      601 tttcaacggt gcgccggagc ggccgtcggc gatggtgaac gtgccgggct caggataata
      661 gaaccaggtg tagctgagcg catcgccatc gggatcggtc gaaccctcgg cgctgagttc
      721 gacgcgctgg ccgggacggg cagtgagttg cggcgggtgg gcgagtttcg gttgcggcgg
      781 atgattggct tcggcgaagg gtttgatcgt ccaatccata cgggtggcga aatcgttttg
      841 catggcggag cgccagcgcc agatcgtggc ttgattactg gtgtgccagt ggccgtcgac
      901 gccgaggacc tcgtcgtcgg cgtcggtcca gatggggcgg gtttcgggtt cgacgaacca
      961 tttgcgcatc ggcggcgtgt agcgttcgta gcggccgccc cagccgcccc aatcggggtg
     1021 ctcgggggcg ttgagaccgt tttcgatgac gaagagaaac gaaggcgagt cgccctccat
     1081 gagatattca acatgtggat actcggcgcc gagcggacct ttgcagcgga tgtgggcgtc
     1141 gagccaggga ttgtcgacga gagtgaaatc agcgccgacg aagcggccgt gaaattgatc
     1201 gccgctgatg ccgttccacg tcgcgtagtg atatgcaccg ccgtcgtgaa cgccggggct
     1261 gacgacgtag aagaggtcgg ggaaattttt gcgaatccat gggccgctgt cgtcctggtc
     1321 ggcgatggca tagacgcgaa gtttgccgat gaactggtcg agtgcttcgg gcgagcgggt
     1381 ggcgcggact ttccagagcg cctgcgcgag gacgttgggg ccgccccaga cgagcaccca
     1441 gacgggacgc gagtcgtcgc ggtcgacgga ggcgatcaat tgggtggagc cgggagagtc
     1501 catggcttcg ccgacggcgg tcatgccgta agcggggcgg ccttcgctta cgacggaaag
     1561 gagatgctcg gcggtgggat aaccgggctc gtggaggagg agattatcgc ggaccttcgc
     1621 gtag
//
LOCUS       DASPLT010000559         3482 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_46240, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000559 DASPLT010000000
VERSION     DASPLT010000559.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 3482)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3482)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3482
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_46240"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1881)
                     /locus_tag="VEA63_05785"
     CDS             complement(<1..1881)
                     /locus_tag="VEA63_05785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009512207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HYD83538.1"
                     /translation="MPAPKDLPIYELENAVDASLRAAGRLIVQAPTGSGKSTQIPQML
                     LAHGLLGEKGEVVVLQPRRLAARMLAKRVAEEVGTNLGDVVGYQIRLESRVSAKTRIR
                     FVTEGILLRQMSFDATLRGVSAVVFDEFHERHLYGDISLARAIQIQQTTRPDLKIVVM
                     SATLDAGALKEYLAPCDVLVSQGRSFPVRIEYLPKAVNFDNEPVWDVAMRECERIAGE
                     VSGDMLVFMPGGYEISRTVQAIQGSRALRDFVCFPLHGELPPEQQDRAVERYEKRKII
                     VSTNVAETSLTIEGVTAVIDGGLARVARFDPHRGINTLLIEKISAASADQRAGRAGRT
                     APGVCVRLWTEREHAQRAPQELPEVRRLDLSEVVLTLKASGIDDVFAFPWLEKPDAKA
                     LERAERLLADLGAVGRDASPSRPPDASERRPYRITDIGRKMLRFPVHPRYARMLLAAQ
                     ERGCVRPVALMAALTQGRNFLLRGAARDVDDARENALGEEHESDFFLLMRAWRYAHQN
                     NYSLEACRRLGIHAQGARQVGPLFEQFLEIARKEGLDVGETRVEGAEVRKCVLAGFSD
                     QLAKRLDGGTLRCELVHNRRGVLARESAIQKSALFVAAEISEIEGRGGEINVLLSLAT
                     AIE"
BASE COUNT          669 a         1198 c         1002 g          613 t
ORIGIN      
        1 ctcgatggcc gtcgccagcg aaagcagcac gttgatttct ccgccccggc cttcgatctc
       61 gctgatttcc gcggccacga acaacgccga tttttgaatc gcactttccc gcgcgagcac
      121 cccgcgacgg ttgtgcacga gttcgcaacg cagcgttccg ccatcgagcc gcttcgccag
      181 ttgatcggaa aaacccgcga gcacgcactt gcgcacttcg gcgccctcca cgcgcgtttc
      241 gccgacgtcg agaccttcct ttcgcgcaat ttccaaaaac tgctcgaaca gcggcccgac
      301 ttgccgcgcg ccctgcgcgt gaatgccgag tcggcggcac gcctccagcg aatagttgtt
      361 ctggtgcgca taacgccacg cgcgcatcag cagaaaaaaa tcgctctcgt gttcctcgcc
      421 gagcgcgttt tctcgtgcgt cgtcgacgtc gcgcgctgcg ccgcgcagca gaaaatttcg
      481 cccctgcgtg agcgccgcca tcaacgccac cggtcgcacg caaccgcgtt cctgcgccgc
      541 caacagcatg cgcgcataac gcggatgcac cggaaaccgc agcatcttcc gaccgatgtc
      601 cgtgattcgg tagggacgcc tctccgaggc gtccggcggt cggctcggag aggcgtccct
      661 acccaccgcg cccaaatccg ccagcaaccg ctccgctcgc tcgagcgctt tcgcatccgg
      721 tttctcgagc catggaaacg caaacacgtc gtcgatcccg ctcgccttca acgtcaacac
      781 cacctccgac aaatccaacc gccgcacctc cggcagctcc tgcggcgcgc gttgcgcgtg
      841 ctcgcgctcc gtccacaacc gcacgcacac gcccggcgcc gtgcggccgg ctcgacccgc
      901 gcgttgatcc gcactcgccg ctgaaatctt ctcgatcagc agcgtgttga tcccgcgatg
      961 cggatcgaaa cgcgccacgc gcgccagccc gccatcgatc accgcggtca cgccttcgat
     1021 cgtcagcgac gtttccgcga cgttcgtcga cacgatgatc tttcgtttct cgtaacgttc
     1081 caccgcgcga tcctgttgct ccggcggcaa ctcgccatgc agcggaaaac acacgaaatc
     1141 gcgcaacgct cgcgagccct ggatcgcctg caccgtgcga ctgatttcat agccgccggg
     1201 catgaacacg agcatgtcgc cactcacctc gcccgcgatc cgctcgcact cgcgcatcgc
     1261 cacgtcccac accggctcgt tgtcgaaatt caccgctttc ggcagatact cgatgcgcac
     1321 cggaaaactc cgtccttgcg acaccagcac gtcacacggc gcgagatact ccttcaatgc
     1381 gcccgcatcc agcgtcgccg acatcaccac gatcttcaga tcaggccgcg tcgtctgctg
     1441 aatctgaatc gctcgcgcga gcgagatgtc gccatagaga tgccgctcgt gaaactcgtc
     1501 gaacaccacg gcgctcaccc cgcgcaaggt cgcatcgaac gacatctgcc ttagtaaaat
     1561 tccctccgtc acgaaacgaa tccgcgtctt cgcgctcacg cgcgactcga gacgtatctg
     1621 ataacccact acgtcgccca ggttcgtccc gacttcttcc gccacccgtt tcgcgagcat
     1681 gcgcgcggca agccttctcg gttgcagcac cacgacctcg cccttttcgc cgagcagccc
     1741 gtgagcgagc agcatctgcg gaatctgcgt ggatttgccg gagccggtcg gcgcctgcac
     1801 gatgagccgg ccggcggcgc gcaaggaggc gtcgacggcg ttctccagtt cgtaaatcgg
     1861 aaggtctttc ggagcgggca tggcggccgg caaagctcca agctccaaac cccaagctcc
     1921 agagaatttt caaacttcaa acatgcttcg tttttgctcc gcgcaaagcg gctgagtttt
     1981 gatgtttccc tggagcttgg atgttggagc ttggagcttc cgccgcagca gcggcggccg
     2041 gtgaataacc ggtgaaccac tttgctccca aattactcac gggcggcggc ggtgcatcga
     2101 ttgtgaagaa caaagacttc aatttcgccg gcgcgcctgc acgctctcac tcgttccttg
     2161 atagaacagt cgcagcgcga agacctgaaa aaaccttcga gagcgcgact gggataatcc
     2221 cgggcgaccg gggaaaggtt ggcgacagct ggtgtcgccc tcccctgcga cgcggcgcga
     2281 gccgatgtgg caggcagata tcaccagctc ctctgatccg gagtcgtgtc cggtcagttt
     2341 acacgaacca ggagctgagc ggcgagcggc gattagaaac cgcgcgcgag ccgagtcagg
     2401 cccacaggga gtttcgacct gtgcgccgac gcagttaccc cagagggttt tggttgctac
     2461 tgcgcagacc agaacgcagt tgcgtttccc ggcgggacga ccccgccaaa aggccccgtt
     2521 cgcacgaacc gggcgcgcaa gggaggcgcg attgcgggga gagagcgagc ctggcaaagc
     2581 tcgcgaacct cctcgattgg ggcggcccga aaaacgggtc accccactgc aaaagcgaaa
     2641 gcaacctgag agttaagagg tcagacccgt aacgcggggc caacgtccgg ccaaaagccg
     2701 gagccgaccc cgtggcaagg gtgccgacgc gagagattag aaattgcagg cgtgtctgca
     2761 ccacgtccgg cgagccgctt ccggcaggaa gcgaccgaac tgggcgaaga ggcctcactc
     2821 gaaagattcc gaccggaaac gatcggaacg gtcccgccat gagaccgaaa catccgcatg
     2881 ccgcggagag tggcgtcggg gagcacacgg cccgcgaaag cgaggcgccc aatgtgtgcg
     2941 tcgggaaaga gcgtgtggcg aacgcccatc cccaaatttg gctccgggac cgcaaggtcc
     3001 cggggcctta ttttttttgc gtaaactgct cgcaacctgt ccggtgcgat cggcttcagc
     3061 cacgcccatt tattccgcaa aacgaacctg accgccgccc ggagtgcgca cagcgcgtgt
     3121 agcgaaacgc gtgagcgttt cggtagcgag atgcgcgagt gtcggcagcg aaacgcgtga
     3181 gcatttcgtt ggtgcaatcg ccgaaaagct cacgcttttc gctacgtgcg aactcgcggg
     3241 ccacgcggcc cgccgaccgc cagatcagcg attcgcgtca accgtcgccg tttcgcgctc
     3301 accccagccc aagctgtgac gataattggc agcagtgcca ccgactgcgc cgcttccctg
     3361 gctgcttcgg ccgctgacgc tcgcgctggt cagcggcgcg ctcggctttg tcgcaaaccg
     3421 atggtcgttg cctgttttcg gcgacaccac gctcgttttc ggcggagtct tctcgctggt
     3481 gg
//
LOCUS       DASPLT010000560         1992 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_89167, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000560 DASPLT010000000
VERSION     DASPLT010000560.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1992)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1992)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1992
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_89167"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            351..1046
                     /gene="araD"
                     /locus_tag="VEA63_05790"
     CDS             351..1046
                     /gene="araD"
                     /locus_tag="VEA63_05790"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase AraD"
                     /protein_id="HYD83539.1"
                     /translation="MLEDLKREAYEANLALPREGLINLTFGNASAIDRAKGIFAIKPS
                     GVDYAALKPEHMVLVDLDGKKVEGSLNPSSDTPTHRRLFLAFEHIGGVVHTHSSHATA
                     FAQAGRAIPCFGTTHADYFFGEVPVTRKMTAEEIARAYEWETGNVIVEHFTTNSLSAV
                     DFSAILVNRHAPFTWGATVAKAVEVAVALECCAHMALMSLQIFPTLAPIEPELLNKHF
                     NRKHGPGAYYGQK"
     gene            1158..1490
                     /locus_tag="VEA63_05795"
     CDS             1158..1490
                     /locus_tag="VEA63_05795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD83540.1"
                     /translation="MNSSSSWTSDKYIETVREVLRRSTQDKAFRQQALADADKTLESV
                     SGIAIPADKRGKAKFVEQAGGKGHVLPAFGAKFEPDQLSDAELERVAGGGSPWCWFTS
                     GCYCFFTK"
     gene            complement(1635..1877)
                     /locus_tag="VEA63_05800"
     CDS             complement(1635..1877)
                     /locus_tag="VEA63_05800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD83541.1"
                     /translation="MNRRSLTPRVREALRRLAAGPQDMRDEALLREINDEMKKLPVEE
                     IAYVRDRVRYSCPPCPMVQSVVLMLNGLIEVKNLQL"
BASE COUNT          409 a          664 c          557 g          362 t
ORIGIN      
        1 ggttacagtt acagtgtgac caaggaaatg ctcgaagact tcgcgacgat tgccgggatc
       61 gagctcgtgg tgatcgatgc cgacacgcgc ctcaccgact tcaaacaagc ccttcgcaac
      121 aacgaggtct actatcacct cgcccaaggc atccgggtgt aagaaaattc cgggtctggt
      181 tacacagagg gcaccgaggt ggcacggagg ccactgagac cttgttcttt cttaaaaaac
      241 ggccacgcga atgcacctcg actggcagct agctttgatc ctccgtgctc tctgtgccac
      301 ctccgtgacc tctgtgtaac cagtccgaat aaacgtccga ttgccctccg atgctcgaag
      361 atctcaaacg cgaagcctac gaagccaacc tcgccctccc gcgcgaaggt ttgatcaacc
      421 tcacctttgg caatgcgagc gccatcgatc gcgcgaaggg catcttcgcg atcaagccga
      481 gcggcgtcga ctacgccgcg ctcaagcccg agcacatggt gctcgtcgat ctcgacggca
      541 agaaagtcga aggctcgctc aacccgtcct ccgacacgcc cacacatcgc cggctctttc
      601 tcgccttcga gcacatcggc ggcgtcgttc atacccactc gtcgcacgcg accgctttcg
      661 cccaagcggg ccgcgcgatc ccgtgctttg gcaccacgca cgccgattat ttcttcggcg
      721 aagttccggt cacccgcaag atgaccgccg aggaaatcgc ccgcgcctac gaatgggaaa
      781 ccggcaacgt catcgtcgag cactttacga caaactcgct cagcgccgtc gatttcagcg
      841 cgatcctggt caaccgccac gcgccgttca cgtggggcgc gacggtcgcc aaagccgtcg
      901 aagttgccgt cgcgctcgag tgctgtgcgc acatggcgct gatgagtctc caaatttttc
      961 ctacactcgc gccgatcgag cccgagttgt tgaacaagca tttcaaccgc aagcacgggc
     1021 ccggcgctta ctacggccaa aagtagcttc tggagtgcgg tggctcgacg ccgcactcca
     1081 gagggcgagt tgagacttgc cgcacgggac ttggaggata caccaaccac cgcaattttc
     1141 acccctgaca aaatcccatg aactcctcct ctagctggac ctccgacaaa tacatcgaga
     1201 ccgtgcgtga agtgctgcgc cgttcgactc aggacaaggc gttccgtcag caggcgctcg
     1261 ccgatgccga caagacgctc gaaagcgtga gcggcattgc gattcccgcc gacaaacgcg
     1321 gcaaagccaa attcgtcgag caggccggcg gcaagggtca cgttcttcct gccttcggtg
     1381 ccaagttcga gcccgaccag ttgagcgatg ccgagctcga gcgcgtcgcc ggcggcggca
     1441 gcccgtggtg ctggttcacc agcggctgct actgcttttt caccaagtaa tgttttttcg
     1501 tgcgccggtt cgatgaggcg cacgccagac cagaaccccg ccgcgtccca ttgctgcggg
     1561 cgggggccga gccgaaacaa aggagacgaa ctcgtgcgtc gtgcgcgcgt tttgcgaacg
     1621 acgccatctc ccgcctagag ctggagattt ttcacctcga tcagcccgtt cagcatcagg
     1681 acgaccgact gcaccatcgg acaaggcggg caggaatacc ggacgcgatc gcgaacgtaa
     1741 gcgatctcct caaccggcag cttcttcatc tcgtcgttga tctcgcgcag cagcgcctcg
     1801 tcgcgcatgt cctgcgggcc tgccgccaac cggcgaagcg cctcgcgaac gcggggagtg
     1861 agtgaacggc ggttcatgac gagtaaggcc gcggaacgtg cacacctcac cgcgaatggc
     1921 cagccccgcg ccacggagat ggctaacctc cacgttcgag cgcctctgca aactgacttg
     1981 gtgccgcgct cc
//
LOCUS       DASPLT010000561         1454 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_89191, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000561 DASPLT010000000
VERSION     DASPLT010000561.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1454)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1454)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1454
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_89191"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(447..899)
                     /locus_tag="VEA63_05805"
     CDS             complement(447..899)
                     /locus_tag="VEA63_05805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD83542.1"
                     /translation="MKRFLFLPILLFAALAAGCSTFEKRSQEKAATFESLTPEEREKL
                     KRGVIEIGNNPDMVYIALGRPDQTRETSTAEGRETIWIYNTYHREYEGQFHSGYRRIL
                     VYDPVRKRYTVFYDPVYTDVYSNHTEEHIRIKFLDDRVVEIEQPKPPV"
     gene            complement(962..>1454)
                     /locus_tag="VEA63_05810"
     CDS             complement(962..>1454)
                     /locus_tag="VEA63_05810"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HYD83543.1"
                     /translation="SKLHRIGQRAPRPSLEQNPFVMPIALAETDADIARCFPVMVQLR
                     PHLTSADFVARIRRQQKENYRLAFATDADREVRSVAGFRVMEMLYSGRTLYVDDLVTD
                     AAARSQGHGDRLLDWLVAHARSLDCDEFSLDSGTQRVDAHRFYLRKRMKIASFHFSLR
                     LKP"
BASE COUNT          279 a          338 c          566 g          271 t
ORIGIN      
        1 ggtggtgtcg agccggtcgc gggcgagcag ttctgcgtag aagccgtcga aattcagcac
       61 gtcgggatga ggcgcgagca gttgggcgac ctcgttgagg gcagaggtgc gcttggtgtt
      121 tttcacctgc agctgaatgc gggcgggatc gagaagttga gagaggcggc cgggcattgc
      181 gaatcgggcg gagatgctcg ccgagagtga cgcgcgctac aaaattgttg gcaagcgtgg
      241 agtcggcttt gcggagaatt agagtgcgcc gcgacgagca agcgaatggc tcgagcgtgc
      301 gcgcgagaga aggatgacgg tgaacgctgg aagaaagcgc cgggcgacgg cgcgaggggc
      361 gcggtggagg gccgcgcatg tgcgtcggtg agcgagacgc gagcgaacgg cgtgtcggcg
      421 gcacgccgga gggcgcggaa cgaagatcaa acgggcggct tcggctgctc gatttcgacc
      481 acgcgatcgt cgaggaattt tatgcggatg tgttcctcgg tgtgattgct gtaaacgtcg
      541 gtgtagaccg gatcgtagaa aacggtgtag cgcttgcgaa cggggtcgta gacgagaatg
      601 cgtcgatagc cggagtggaa ctggccctcg tattcgcgat ggtaggtgtt gtagatccag
      661 atggtttcgc ggccttcggc ggtggaggtt tcgcgtgtct ggtccgggcg gccgagcgcg
      721 atgtaaacca tgtcggggtt gttgccgatt tcgatgacgc cgcgtttgag tttctcgcgt
      781 tcttcgggcg tgagggactc gaacgtcgca gctttttcct gcgagcgttt ttcaaaagtg
      841 gagcagcccg cggcgagtgc ggcgaagagc aggatgggga gaaagaggaa gcgtttcatg
      901 tgaactagga cggggaacgt ggctcagggt taccgcgctc gcaagcgacc gccggcggga
      961 atcagggctt caatcgaagc gagaagtgga aggaggcgat tttcattcgc ttccgcagat
     1021 agaaacgatg cgcgtcgacg cgttgggtgc cggagtcgag ggagaactca tcgcaatcaa
     1081 gcgagcgggc gtgcgcgacg agccagtcga gcaagcgatc gccgtggcct tgcgagcgag
     1141 cggcggcatc ggtcacgaga tcgtcgacgt agagcgtgcg gccggagtag agcatttcca
     1201 tcacgcgaaa gccggcaacg ctgcggactt cgcggtctgc atcggtggcg aacgcgaggc
     1261 ggtagttttc cttttgttgg cgacggatgc gggcaacgaa atcggcggac gtcaggtgcg
     1321 ggcgcagttg caccatcacg ggaaaacagc gggcgatatc ggcgtcggtt tcggcgaggg
     1381 cgatgggcat gacgaatggg ttttgttcca gcgaggggcg cggcgcgcgt tggccgatgc
     1441 gatggagttt cgat
//
LOCUS       DASPLT010000562         1611 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_108294, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000562 DASPLT010000000
VERSION     DASPLT010000562.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1611)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1611)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1611
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_108294"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1611)
                     /locus_tag="VEA63_05815"
     CDS             complement(<1..>1611)
                     /locus_tag="VEA63_05815"
                     /inference="COORDINATES: protein motif:HMM:NF028498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 30 beta sandwich
                     domain-containing protein"
                     /protein_id="HYD83544.1"
                     /translation="DYSREFAGKRAPIAVMGPDIEQNIAVFDYEHTLPKGSGVLASVG
                     TQRAAELGDFKLLGCFWSPAPWVKIASGNVSPNYGGILPVQGTPWPYICLDNFAGGKL
                     DVSHTPLEVFNDGTGPTSALTQYARSCAAYILGYQRHFGVKFYAVSIQNEPGFEQYYN
                     SMKHALSSSYIAALNALRAEFDRHAELRHIVFSGTDDLLGNDAYGLWQYGGGATTQHK
                     NLQFLANIEADPTARRALQIYAIHGYAANGVNAAEADPLQWRWWANGWTESPAPGVPA
                     PVAGFVSTGKRSWMTETSGENPQWLWPQSGFPGSGAWSVALRIHQALTAGRQSAWLYW
                     QMADGGETSAGETLTGSMELETAPKFNAAKHFFRYIRPGAQRVEVEVNSAAIDASAYV
                     HDDNQTITYVLLNTTGTPVTLDVAAPALPSGVENFEVFTSSEANYWIPSAAAVVDGKA
                     SVSVPAYGVVTLVGAGTSRSVFGDRLGDGWTRADGAITSESTHWGSERALNFETSRKH
                     RTLELTRASGLALNGNDILEFYVRAMAAK"
BASE COUNT          278 a          566 c          454 g          313 t
ORIGIN      
        1 tttggccgcc atcgctcgaa cgtagaattc taaaatgtca tttccgttca gcgccagccc
       61 gctggcacgc gtaagctcga gcgtgcggtg cttgcgactc gtctcgaaat tcaacgcccg
      121 ttcgctgccc caatgcgtgg attcgctcgt gatggcgccg tcggctcgcg tccacccgtc
      181 gccgaggcgg tcgccgaaaa cactccgcga ggtccccgct ccgacgagcg tcaccacgcc
      241 ataagcgggc acgctgaccg acgccttgcc gtccaccacc gctgccgccg acggaatcca
      301 gtagttcgcc tcgcttgacg tgaacacctc gaagttttcc acgccgctcg gcagcgccgg
      361 cgccgccacg tcgagcgtca ccggtgtccc cgtcgtgttc agcagcacat acgtgatcgt
      421 ctggttatcg tcgtgcacat aggcgctcgc gtcgatcgcc gccgagttga cttcgacctc
      481 gacgcgttgc gcgccgggac ggatgtagcg gaagaaatgt ttcgccgcat tgaacttcgg
      541 cgccgtttcg agctccatcg atcccgtcaa cgtctcccct gccgacgtct caccgccgtc
      601 cgccatttgc caatacaacc acgcgctctg acgccccgcc gtgagcgcct gatgaatccg
      661 cagcgccacg ctccacgcgc ccgagcccgg aaatccgctc tgcggccaaa gccactgcgg
      721 attttcgccg gacgtctcgg tcatccacga tcgcttcccg gtggaaacaa aacccgctac
      781 tggcgctggc acacccggcg ccgggctctc ggtccatccg tttgcccacc accgccattg
      841 caacgggtcc gcttccgccg cgttcacgcc gttcgccgcg taaccgtgga tcgcataaat
      901 ttgcagcgcc cgccgcgcgg tcggatcggc ttcgatgttc gcgagaaatt gcaggttctt
      961 gtgctgcgtc gtcgcgcctc caccgtattg ccaaagtcca tacgcatcgt tgccgagcag
     1021 gtcgtccgtg cctgagaaca cgatgtgcct aagctctgcg tgccggtcga actccgcgcg
     1081 caacgcattc agcgcggcga tgtagctgct cgagagcgcg tgcttcatgc tgttgtagta
     1141 ttgttcgaaa cccggctcgt tctggatgct cacggcgtaa aattttactc cgaaatgccg
     1201 ctgataaccg agaatgtagg ccgcgcagct tcgcgcgtat tgcgtcaacg cactcgtcgg
     1261 ccccgtgccg tcattgaaca cctcgagcgg cgtgtgcgaa acgtcgagtt tcccaccggc
     1321 gaaattatcc aggcagatgt aaggccaggg cgtgccctgc accggcagga tgccgccgta
     1381 gttcggcgag acgttgccgg acgcgatctt cacccacggc gccggcgacc aaaagcaacc
     1441 gagcagtttg aaatcgccga gttcggccgc gcgctgcgtc ccgacgctgg ccaggacgcc
     1501 tgaacctttc ggcagcgtgt gctcgtaatc gaacaccgcg atattctgct cgatatccgg
     1561 ccccatcacg gcgatcggcg cccgcttccc cgcaaactcg cgcgaatagt c
//
LOCUS       DASPLT010000563         3757 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_150057, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000563 DASPLT010000000
VERSION     DASPLT010000563.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 3757)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3757)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3757
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_150057"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            32..1183
                     /locus_tag="VEA63_05820"
     CDS             32..1183
                     /locus_tag="VEA63_05820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009511074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="complex I subunit 1 family protein"
                     /protein_id="HYD83545.1"
                     /translation="MLADLYLNLHPVAQGFLKGLAVILVIFPIAGACSMAERKVAAWV
                     QDRPGPNRAIVPWVAWIPFIGRFLQRLGIFHVMADGGKMLFKEDSTPNHVNKFYFISA
                     PLLAMVPALTTVTVVPFGAYIDALGDVVPLGLAPVDIGILAVFAISSLSVYSLILAGW
                     ASNSKYPFLGSVRASAQLISYELSMTLAVLPVFLWVNAPGLAGTMGFGRVVEFQSQPG
                     FLGGMWFIFIMPVSAFIFLTALFAETNRQPFDTVESEADLVGSFHTEYGAFKWGLFFV
                     AEYSHMIVGSAVFVLLFCGGWNPLPWVSLATFADWLKIADNPIVLGLVSIAIFVGKTL
                     AMIFFFMWVRWTVPRFRYDQVMKLGWQKLLPASIANLLFYAIAIAFIQK"
     gene            1292..1840
                     /locus_tag="VEA63_05825"
     CDS             1292..1840
                     /locus_tag="VEA63_05825"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007360957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit I"
                     /protein_id="HYD83546.1"
                     /translation="MAVVPLERKPLTFAERTYVPQIVSGLKTTFKHLVRKKQTLEYPE
                     QRPEIPTGYRGVPTLVKDPNGREKCVSCQLCEFVCPPKAIRITPGEIDPAAPTSHVEK
                     APREFEIDMLRCIYCGMCQEVCPEEAIFLQQQYTMTGYTRAEMVNNKERLYELGGTLP
                     DQHFKWDKKKAAEQAASAQHHH"
     gene            1943..2458
                     /locus_tag="VEA63_05830"
     CDS             1943..2458
                     /locus_tag="VEA63_05830"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012709.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HYD83547.1"
                     /translation="MSPLLFNIFALLTVIAAVATVLNKNAVNAAMCFMLSLVGVAGLF
                     VMLEAYLLAFVLVLVYAGAVAALFLFIVMLLNVQQAASKPFKKMTLVASTVAAALLAV
                     GVLAFYRQPELTTGALSAAPAVGASLKDYSYQLFTTYLLPVQIIGFLLLIAMLGVIVL
                     SRKMDNAEPAS"
     gene            2455..2763
                     /gene="nuoK"
                     /locus_tag="VEA63_05835"
     CDS             2455..2763
                     /gene="nuoK"
                     /locus_tag="VEA63_05835"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HYD83548.1"
                     /translation="MITASLHTYLVLSALLFAIGFFGVLLRKNTLIIFMGLELMLIAS
                     TLALVAFSRYNGTMDGNVFVFFILTVAAAEVAVGLAIIVALFRKRGTIEVDQLDGLKN
                     "
     gene            2930..>3757
                     /locus_tag="VEA63_05840"
     CDS             2930..>3757
                     /locus_tag="VEA63_05840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HYD83549.1"
                     /translation="MTMPQLAVTVLLLPLASAALIALFLRRAGSLAALISVLAATGIA
                     AGSLYLALGNHRFDTALEWLRLGDFALSLGIKFDDLAALMLVIVGIVGLCVHVFSLGY
                     MHDDAAKARFFGGLSIFMFSMIGIVLADNLFMMFIFWELVGFSSWLLINHWHERPSAA
                     AASKKAFIVNRVGDFGFLLGIIMCYWVNGTVSLTELGQGSAIASTAIPLLLFCGAIGK
                     SAQMPLQVWLPDAMEGPTPVSALIHAATMVAAGIYMLCRINALMVPDALTVIMWIGTA
                     "
BASE COUNT          612 a         1271 c         1028 g          846 t
ORIGIN      
        1 cacgttcttc atctttcgct aatcgcccgc catgctggct gacctctact taaacctgca
       61 cccggttgcg caaggctttc tcaagggcct cgcagtgatc ctcgtgattt tcccgatcgc
      121 cggcgcgtgc tcgatggccg agcgcaaagt cgccgcgtgg gtccaggatc gtcccggtcc
      181 gaatcgcgcg attgtgccgt gggtcgcttg gattccgttc atcggccgct ttctccagcg
      241 cctcggtatt tttcacgtga tggcggacgg cggtaagatg ctcttcaagg aagactccac
      301 gccgaaccac gttaacaagt tctacttcat cagcgcgcca ctgctggcga tggtgccggc
      361 gctgaccacc gtcacggtcg tgccgttcgg cgcttatatc gatgcgctcg gcgatgtcgt
      421 gccgctcgga ctggccccgg tcgacatcgg catcctcgct gtcttcgcga tctcgtcgct
      481 cagcgtttac tcgctgatcc tcgcgggctg ggcctccaac tcgaagtatc catttcttgg
      541 atccgtgcgc gcgtccgcgc agctcatttc ctacgagctt tcgatgacgc tcgccgtgct
      601 gccggtgttt ctctgggtca acgccccggg cctcgcgggc acgatgggtt tcggccgcgt
      661 cgtcgagttc cagagtcaac ccggcttcct gggcggcatg tggttcatct tcatcatgcc
      721 ggtgtcggct ttcatttttc tcaccgcgct cttcgccgaa accaaccgcc agccgttcga
      781 caccgtcgag tccgaggcgg atctcgtcgg cagcttccac accgaatacg gcgcgttcaa
      841 atggggcctc ttcttcgtcg ccgaatactc gcacatgatc gtcggctccg cggtgttcgt
      901 cctgctcttc tgcggcggct ggaatccgtt gccgtgggtg tcgctcgcga cgttcgccga
      961 ttggctaaag atcgcggaca acccgatcgt gctcgggctc gtttccatcg cgatcttcgt
     1021 cggaaagacc cttgcgatga tcttcttctt catgtgggtc cgctggaccg ttccgcgttt
     1081 ccgctacgat caagtaatga agctcggctg gcagaaactc ctgccggcct ccatcgccaa
     1141 cctcctcttc tacgcgatcg cgatcgcgtt cattcagaaa taagaaacca cgaatggaca
     1201 cgaatgcaca cgaatcagga accgggctga gcagtgtctc ggttcattcg tgtctattcg
     1261 tgtccattcg tggttaaaaa aatctccgcc catggccgtc gtccctctcg aacgcaaacc
     1321 gctcaccttc gccgagcgca cctatgtccc gcagatcgtc agcgggctga agacgacgtt
     1381 caaacacctc gtccgcaaga aacagacgct cgagtatccg gagcagcgtc cggaaattcc
     1441 caccggctac cgcggcgtgc cgacgctcgt gaaggatccg aacggccggg agaagtgcgt
     1501 ttcctgccag ctgtgcgaat tcgtctgccc gccaaaagcg atccgcatca cgcccggcga
     1561 aatcgatccc gccgcgccga cctcgcacgt cgagaaggcc ccgcgtgaat tcgagatcga
     1621 catgctccgc tgcatttact gcggcatgtg ccaggaggtc tgcccggaag aggcgatctt
     1681 ccttcagcag cagtatacaa tgactggata cacgcgggcc gagatggtga acaacaagga
     1741 gcgcctctac gaactcggcg gcacgctgcc ggaccagcat ttcaaatggg acaagaagaa
     1801 agccgccgag caggccgctt cggcgcagca tcaccattga gaatcatgcc taaacctcgc
     1861 acctccactg tagccgcgcg cgtgagcgcg cggatcgcac cgtgaccggt gcggctaccg
     1921 agtcagcgac gccttcgcct ccatgtctcc tcttctgttt aacatcttcg cgctcctgac
     1981 ggtcatcgcc gccgtcgcca cggttctgaa caagaacgcc gtcaacgccg cgatgtgttt
     2041 catgctgagt ctcgtcggcg tcgccgggct cttcgtcatg ctcgaagcct acctcctggc
     2101 cttcgtgctc gtcctcgttt acgccggcgc cgtcgctgcg ttgttcctgt tcatcgtcat
     2161 gttgctcaac gtgcagcaag cggcgtccaa gcccttcaag aagatgacgc tcgtcgccag
     2221 caccgtcgcc gccgcgctgc tcgcggtcgg cgtgctcgcg ttctaccgcc agccggagct
     2281 caccaccggc gcgctctccg ccgcacccgc cgttggcgcc tcgctcaagg actactccta
     2341 ccagctcttc accacgtatc tgctccccgt gcagatcatc ggtttcctgc tcctcatcgc
     2401 gatgctcggc gtgatcgtgc tgagccggaa gatggacaac gccgaacccg cgtcatgatc
     2461 accgcatcgc ttcacactta tctcgtcctg agcgcgctgc tctttgcgat cggcttcttc
     2521 ggcgtgctgc tgcgcaagaa cacgctgatc attttcatgg ggctcgaact gatgctgatc
     2581 gcgtcgaccc tcgcgctggt cgccttctcg cgttataacg gcacaatgga cggcaatgtt
     2641 ttcgttttct tcatcctcac cgtcgccgcc gccgaagtcg cggttggtct cgccatcatc
     2701 gtcgcgctct tccgcaaacg cggcaccatc gaagtcgacc agctcgacgg tctgaagaac
     2761 tgaactcgga atgttacaca gagcacaccg agcaccgaca cggagttcac ggagtaattc
     2821 aaaatgactt ggagccccaa ccgccctctc gctttcgtcc accaccggga ctccgtgtcc
     2881 tccgtgggcc gcgctccgtg ccctctgtgt tcctgatcga aatcgcgcca tgacgatgcc
     2941 ccagctcgcc gttaccgttc tccttctgcc gctcgcgtcc gcggcgctga tcgcattgtt
     3001 cctgcgtcgc gccggctcgc ttgctgcgct catttccgtg ctcgccgcga cgggcatcgc
     3061 cgccggctcg ctttatcttg cgctcggtaa ccaccgtttc gatacggcgc tcgaatggct
     3121 gcgtctcggc gacttcgcgc tctcgctcgg catcaagttt gacgatctcg ccgcgctgat
     3181 gctcgtgatc gtcggcatcg tcggcctttg cgtgcatgtt ttctcgctcg gctacatgca
     3241 tgacgacgcg gcgaaagcgc gcttcttcgg ggggctttcg atcttcatgt tttcgatgat
     3301 cggcatcgtg ctcgccgaca atcttttcat gatgttcatc ttttgggagc tggtcggttt
     3361 cagctcttgg ctgctgatca atcactggca cgaacgccct tccgccgccg ctgcctcgaa
     3421 aaaggccttc atcgtcaacc gcgtcggcga cttcggcttt ctgctgggca tcatcatgtg
     3481 ctactgggtc aacggcacgg tcagcctcac ggaactcggc cagggcagcg ccatcgcgag
     3541 caccgcgatt ccgctgcttc tcttctgcgg cgcgatcggt aaatccgcgc agatgccgct
     3601 gcaagtctgg ctcccggacg cgatggaagg cccgacaccc gtgtccgcgc tgatccacgc
     3661 cgcgacgatg gtcgcggccg gtatctacat gctctgtcgc atcaacgccc tgatggtgcc
     3721 tgacgcgctg accgtcatca tgtggatcgg caccgcg
//
LOCUS       DASPLT010000564         1023 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_171437, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000564 DASPLT010000000
VERSION     DASPLT010000564.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1023)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1023)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1023
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_171437"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..573
                     /locus_tag="VEA63_05845"
     CDS             <1..573
                     /locus_tag="VEA63_05845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD83550.1"
                     /translation="GGVAGNPNNVSPTVSPDLANAITQYNNLRAAYVNLTPADQKVAR
                     LAEITANLFTDYTFQTGVLKNFRVGGGVNYRGREVIGYRGGDTIADPSNPAQAIDNPS
                     FDATTPVYRPDYYLVTGVLGYSFKLMNKYPVRLDLTVNNLLEEDMPLYYNTVQRPPNG
                     NIGDPSRIAAPNQYAYLTPRSFTLTATFSF"
     gene            727..>1023
                     /locus_tag="VEA63_05850"
     CDS             727..>1023
                     /locus_tag="VEA63_05850"
                     /inference="COORDINATES: protein motif:HMM:NF012686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD83551.1"
                     /translation="MMLASAGLLIPALPFAAVSGATVSGYDYGIIVVYLIFLASMGWI
                     FRKFTAGSKDYFAGGYRQTWWLLGASSFISNFSCWTFTGAAGIAYTYGFLIFAVY"
BASE COUNT          199 a          302 c          296 g          226 t
ORIGIN      
        1 ggcggcgtcg cagggaaccc gaacaacgtt tcaccgacgg tttcaccgga tctcgcgaac
       61 gcgattacgc agtataacaa cctgcgcgcc gcctacgtga acctcacgcc cgcggaccaa
      121 aaggtcgcgc gtctcgcgga gatcacggcg aaccttttca cggattacac gtttcaaacg
      181 ggcgtgttga aaaacttccg cgtcggtggc ggcgtgaact atcgcggacg cgaagtcatc
      241 ggctatcgcg gcggtgacac gatcgcggat ccgtcgaatc cggcgcaggc gatcgataat
      301 ccgagcttcg atgcgaccac gccggtgtat cgaccggact actacctcgt caccggcgtg
      361 ctcggctaca gcttcaagct gatgaacaag tatccagtgc gtctcgatct gacggtgaac
      421 aacctgctcg aggaggacat gccgctttac tacaacaccg tgcagcgtcc gccgaacggc
      481 aacatcggcg atccttcgcg catcgcggcg ccgaaccaat acgcgtatct cactccgcgc
      541 agtttcacgc tgaccgcgac gtttagcttc taaaacaaac ggccgcgtag ttatgatcgg
      601 aggtaggagc ctgcttgcga gcgatggtgc cccagccgta gtcgaatgcc gagatcgcct
      661 gcaagcaggc tcctacctcc caatcaagcg agcgattgcc atgctcgcac ttagtcagcg
      721 cttttcatga tgctcgcttc cgctggtctt ttgattcccg cgctgccgtt tgcggcggtg
      781 tccggtgcga cggtgagcgg ctacgactat gggatcattg tcgtctatct gatctttctg
      841 gcctcgatgg gctggatttt tcggaagttc accgccggca gcaaagacta cttcgcgggc
      901 ggatatcggc agacgtggtg gttgctcggc gcgagttcgt ttatttcgaa tttcagttgt
      961 tggacgttca cgggcgcagc gggaattgcc tacacgtatg gcttcctgat tttcgcggtg
     1021 tat
//
LOCUS       DASPLT010000565         1626 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_89232, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000565 DASPLT010000000
VERSION     DASPLT010000565.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1626)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1626)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1626
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_89232"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1425)
                     /locus_tag="VEA63_05855"
     CDS             complement(<1..1425)
                     /locus_tag="VEA63_05855"
                     /inference="COORDINATES: protein motif:HMM:NF036578.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD83552.1"
                     /translation="MSQTPFLQVEFSDASPEFSWFAVDSLGRGENLGNVVLTEKLRPG
                     SGRLEPIDDMQARYMGGAADGAQTTTWRMRVDGPRVVLQSDYSTGVRVEPFRLLINQK
                     KNHATLLGLPVDAGHGQRIALPAVLHLPDRGTLRVSASAPGVAVGYDARRRQPEYFVE
                     VLFPAATADCPRIEYVLEATLIHPSLPGLEAQPLYDGYRRSFLNLLQFHSRLRTVANN
                     SSSDVCGFCFWMYAELAVQAPPLAPGLRALDLIRVSLDRVLDGGLTYGQAGYQKTPQY
                     PDAAAWSPPFDSLDTLPSFVIAGAIYLRGSQDRVWAEARFDQLVQLARRMLAQDRNGN
                     GLIEYALSGNSDSWTGDVTTRPANWWDTIGFGHEDAYSNALAFRACSLLADAAEGLGR
                     TAEAGEFHRAAEKLKAAYVSTFYNTQTGVLAGWKSADGKLHDYWFVFVNGMAIAFGLV
                     EEQLAHEIVDRLLAKMKEVGFERFD"
     gene            complement(1444..>1626)
                     /locus_tag="VEA63_05860"
     CDS             complement(1444..>1626)
                     /locus_tag="VEA63_05860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007361416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD83553.1"
                     /translation="MILEDSGEVKLYYGAADTSVALATASLEELLARCEPYDASQKTA
                     GENPLTSRVVLPAGQS"
BASE COUNT          319 a          565 c          468 g          274 t
ORIGIN      
        1 atcgaaacgc tcgaacccta cctccttcat cttcgccagc agccggtcca cgatctcatg
       61 cgccagttgc tcttccacga gcccgaacgc gatcgccatg ccgttcacaa acacgaacca
      121 gtagtcgtgc agctttccgt cggcgctctt ccagccggcg agcacgcccg tctgtgtatt
      181 gtaaaacgtg gatacatatg ccgccttcaa cttttccgcc gcgcggtgga actcgcccgc
      241 ttccgccgtc cggcccaatc cttccgctgc atccgccagc aggctgcacg cgcgaaaggc
      301 gagcgcgttg gagtaagcgt cttcgtggcc gaatccgatc gtgtcccacc agttcgcagg
      361 acgtgtcgtg acgtcgcccg tccagctgtc ggagttgccc gacagcgcgt attcgataag
      421 cccgttgccg ttgcgatcct gggcgagcat ccggcgcgcg agttgcacga gctggtcgaa
      481 ccgcgcctcc gcccaaactc gatcctgcga tcctcgcaga taaatcgctc ccgcgatcac
      541 gaacgaaggc agcgtatcga gcgaatcgaa cggcggcgac caggccgccg catccggata
      601 ctgcggcgtt ttctgataac ccgcctggcc gtaagtgagc cctccatcga gcacgcgatc
      661 gagcgaaaca cggatcagat ccagtgcccg caggcccggc gcaagcggcg gcgcctgcac
      721 cgcgagctcg gcatacatcc aaaaacaaaa tccgcagaca tcgctcgatg aattgttcgc
      781 gacggtgcgc aaccgggaat gaaattgcag caggttcaga aaactgcgcc ggtaaccgtc
      841 atagagaggc tgcgcttcca atcccggcag cgacgggtga atcaacgtcg cctcgagcac
      901 atactcgatc cggggacagt ctgccgtcgc tgccggaaac aggacctcca cgaaatactc
      961 cggctgccgc cggcgcgcgt cgtagcccac cgcaactccg ggcgcgctcg cggaaacgcg
     1021 cagcgttccc cggtcgggca ggtgcagcac cgccggcagc gcgatccgct ggccgtggcc
     1081 ggcatcgacc ggcaacccca gcagggtcgc atgattcttc ttctggttga tcaacagccg
     1141 gaatggctcg actcgaaccc cggtggagta atccgactgc agcacgacac gcggcccgtc
     1201 cacccgcatc cgccacgtcg tcgtttgcgc tccgtcggca gcgccgccca tgtagcgggc
     1261 ctgcatgtcg tcaatcggct caagccggcc cgaacccggg cgcagcttct ccgtcagcac
     1321 aacatttccc agattctcgc cgcgacccag cgaatcgacg gcgaaccagg aaaactcggg
     1381 cgaggcgtcg gaaaactcga cctgcaaaaa gggcgtctgc gacatgggaa atttcaactc
     1441 tcgtcagctc tggcccgccg gaagcaccac gcgcgacgtg aggggatttt cgcccgccgt
     1501 cttttgactg gcgtcatacg gttcgcagcg cgcgagcagt tcctcgagcg acgcggtcgc
     1561 gagcgcgacg gacgtgtccg cggcgccgta ataaagtttc acctcgcccg agtcctcaag
     1621 aatcat
//
LOCUS       DASPLT010000566         2221 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_7305, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000566 DASPLT010000000
VERSION     DASPLT010000566.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 2221)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2221)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2221
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7305"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1732)
                     /locus_tag="VEA63_05865"
     CDS             complement(<1..1732)
                     /locus_tag="VEA63_05865"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HYD83554.1"
                     /translation="MTLSPFEVVSDNRGYYGANTMSGTRFNTKIEDLASAVTIVTKEQ
                     MTDFAMLDINDVFLYTVGAEGTGTYTDIVVDRNGSVSDNVQLNPTQANRVRGLASANV
                     AFNNIEMQNRTPIDPVMIDALEVSRGPNASVFGLGNPSGTVNQVGASANLLRDRYQVQ
                     LRGDDWGGYRETVDVNQVILPEKLALRFSQVFNHEEWERKPSGVDTERYNVMVKYRPF
                     KNTTITGGYYYYHMYGNRPNAIPPRDGISYWRDSGAPTWDPVSREITLNGQVLTRPNG
                     STAWGATNGNYDGPTDALSSGWLGGTHSQVFINENGAITHWTGPTGVSGIGTPQPPNG
                     PNPSGPGVQPNRYLQPTPMAGLGNASTGKGAQALFLTVPSITDRSIFDWKNINISAPN
                     RIWDKSEISTLQLDQIFLNTPRQTLAAQFTFLREESERYQRNFIGIANDLGLSGLLTI
                     DVNERLLDGANNPYFLRPYISTSKPRTTYSPWKWDTYRGQAAYKFDFTQERNLLRHLG
                     IHQLTGYYEYKYRVNRNYSYRDVMTSNNPWITPGVYRGNQSQVANTPTLIATTQSNFR
                     YYVGDNQGYNA"
BASE COUNT          400 a          551 c          812 g          458 t
ORIGIN      
        1 cggcgttata gccctggttg tcgccgacgt agtagcggaa gttgctctgc gtggtggcga
       61 tgagggtggg cgtgttggcg acttgggact ggttgccgcg atagacgccg ggcgtgatcc
      121 aaggattgtt ggaggtcatg acgtcgcggt aggagtagtt ccggttcacg cggtatttgt
      181 attcgtagta accggtgagc tggtgaatgc cgaggtggcg gaggaggtta cgctcctgcg
      241 tgaagtcgaa cttgtaagca gcctggccac ggtaggtgtc ccacttccag ggcgagtagg
      301 tcgtgcgtgg tttcgacgtg ctgatgtagg gacggaggaa gtaagggttg ttcgcgccgt
      361 cgagcaggcg ctcgttgacg tcgatcgtga gcaggccgga gagaccgagg tcgttcgcga
      421 tgccgatgaa gttgcgttga taacgctccg attcttcgcg gaggaaggtg aactgcgcgg
      481 cgagagtctg acgcggcgta ttcaggaaga tctggtcgag ctgcagcgtg gagatctcgg
      541 acttatccca aatgcggttg ggcgcggaga tgttgatgtt cttccaatcg aagatcgaac
      601 gatccgtgat gctggggacc gtgaggaaga gggcctgcgc gcctttaccg gtggacgcgt
      661 tgccgaggcc cgccatcggg gtgggctgca ggtagcgatt tggttgcacg ccgggaccgg
      721 aggggttggg gccgttcggc ggctgtggag tgccgatgcc gctaacgccc gtggggcccg
      781 tccagtgggt gatggcgccg ttttcattga tgaagacttg gctgtgcgtg ccgccgagcc
      841 agccggagct gagggcgtcg gttgggccgt cgtaattgcc gttggttgcg ccccaagcgg
      901 tggagccgtt cgggcgggtg aggacctggc cgttcaacgt gatttcgcgc gagacgggat
      961 cccaggtcgg cgcgccgctg tcgcgccagt agctgatgcc gtcgcggggc gggatggcat
     1021 tcggacggtt gccatacatg tggtagtaat agtagccgcc ggtgatcgtc gtgtttttaa
     1081 acgggcggta tttcaccatc acgttatagc gctcggtatc gacgccagag ggtttgcgct
     1141 cccattcctc gtgattgaag acctggctga aacgcagggc gagtttctcg gggaggatga
     1201 cttggttgac gtcgacggtt tcgcgatagc cgccccaatc gtcgccgcga agttgaacct
     1261 gatagcgatc gcgaaggagg ttggcgctgg cgccgacctg gttcacggtg ccggacggat
     1321 tgccgaggcc gaagacgctg gcattgggac cacggctgac ttcgagcgcg tcgatcatga
     1381 ccggatcgat cggcgtgcgg ttctgcattt cgatgttgtt gaacgcgacg ttggccgagg
     1441 cgagaccgcg cacgcggttg gcctgcgtgg ggttcaactg aacattgtcg gagacggagc
     1501 cgttgcgatc gacgacgatg tcggtgtagg tgcccgtgcc ctcggcgccg acggtgtaga
     1561 ggaacacgtc gttgatatcg agcatcgcga agtcggtcat ctgctccttc gtcacgatgg
     1621 tgacggccga ggcgaggtcc tcgatcttcg tgttaaaacg cgtgccggac atcgtgttcg
     1681 cgccgtaata accgcggttg tcgctgacga cttcgaaggg ggacaaggtc acaatctcat
     1741 catcttcagg agcggacgag gtggtcgcgt cttgcgcccc gagaggcgaa aacgcgaggg
     1801 ggacgagcgc agacgccgcg aggagccggc gcaggttcag ggttggtcga taggcattca
     1861 tattggtttg tagtagctac tgcggaaaaa cagataaccg gaaagtggct gagcgacggc
     1921 cgcgtgaggc ggtaagggcg gagcggcacg ggaggcatgc cgcccactaa cgcgagaagt
     1981 ccgtcgaggc aggggcgggc gcagcgacga gaaaggcaga acgaagagaa gcggttcgat
     2041 tgaaacggtt cgaaggtgaa ccgttgggga attcgatggc cgaagggaat gctcgcactg
     2101 cgcccgctca agagcactgt tgcgcacatt gttgactcgc gcgatcagcg aaacgtccca
     2161 tctccttgcg gcgcaggcat cctcgtcgat agcctgcgat gatttatcgt ttctccagtc
     2221 c
//
LOCUS       DASPLT010000567         4749 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_191853, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000567 DASPLT010000000
VERSION     DASPLT010000567.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 4749)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4749)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4749
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_191853"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            157..525
                     /locus_tag="VEA63_05870"
     CDS             157..525
                     /locus_tag="VEA63_05870"
                     /inference="COORDINATES: protein motif:HMM:NF015735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HYD83555.1"
                     /translation="MKYALLIYLDEKQFTALPKEEQNRVHQECGAWHEELVKSGHSVG
                     CKGLQGTATATTLRERDGKVAIMDGPFAETKEMLGGLETLECRDLDEALGIAKRFPGL
                     RGGRCAVEVRPEVPGGTCEA"
     gene            598..1803
                     /locus_tag="VEA63_05875"
     CDS             598..1803
                     /locus_tag="VEA63_05875"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HYD83556.1"
                     /translation="MSVEEIYRREYGRILASLIRVVRDFTLAEDALQEAFAAAHERWA
                     KEGWPRNPVGWLMTTARWRAIDEIRRRALAENRREEIALISGPQEEAPMPIDELRLIF
                     TCCHPALAPDARVALTLRTVSGLTTEEIARAFLVPVPTMAQRLVRAKTKIKLAGIPYL
                     VPADDELSERLAAVLATIYLVFNEGYAASTGENLVRGELCTAAIRVGRDLVALLPQER
                     EAAGLLALMLLSDARRAARTDAAGELVLLEEQDRTQWDRAKIDEGCALAERALGGASQ
                     PGGYAVQAAIAAVHARAKTAGETDWSGIARWYGLLARIAPSPVVELNRAVAIAMAEGL
                     EKGLALMERIDMPGYYLVPAARADLLRRLGRNAEAAAAYREALALATNEAERRFLMKR
                     IAEVARAAE"
     gene            complement(1808..2017)
                     /locus_tag="VEA63_05880"
     CDS             complement(1808..2017)
                     /locus_tag="VEA63_05880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016293078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HYD83557.1"
                     /translation="MPIQLNLDVVMAKRGISLTELAERVDLTLANLSILKTNKAKAIR
                     FSTLEALCRELRCQPGDLLEFVDEK"
     gene            complement(2021..2713)
                     /locus_tag="VEA63_05885"
     CDS             complement(2021..2713)
                     /locus_tag="VEA63_05885"
                     /inference="COORDINATES: protein motif:HMM:NF022636.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HYD83558.1"
                     /translation="MKLFTNLPGNLRALFGALRLVVVLLAAFWFFTLTFNAWIQKRFV
                     DDPKLIVTVGEVTATESAQPPTLVSTAAGPGALILGDLNGPLQADLLTGDAALVSAVR
                     WTILPAMIAFVVFSWWLFGSLRAVCANIERGQVFTETNLRLVRGIGGLLIAYSLVTLI
                     VGWWGAHVMNDYLAHHVTATGFASSPGALRFSLGGQFPQWGGLVTGLVVLVVSEAFRQ
                     GLTLKTENDLTV"
     gene            complement(3045..3419)
                     /locus_tag="VEA63_05890"
     CDS             complement(3045..3419)
                     /locus_tag="VEA63_05890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HYD83559.1"
                     /translation="MPVALSDIGQIAITVRDVSVSTAFYRDVLGLRFLFAAGPNLAFL
                     AAGSVRLMLTTPQGHGEVGKNSTLYFKVDDLPATHAALIARGAKNERPPQLTAKMPDH
                     DLWMAFLRDPDDNLVGLMSEVR"
     gene            complement(3573..4373)
                     /locus_tag="VEA63_05895"
     CDS             complement(3573..4373)
                     /locus_tag="VEA63_05895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-ketoacyl-ACP reductase"
                     /protein_id="HYD83560.1"
                     /translation="MKKVALVTGGSRGIGFGIAEKLAAEKWDLVINGVRPESAVAEPL
                     AQLRAHGVRVAYAQGDVGSAEGRASILKTCRDFVAAGQAPGSGLSALSLLVNNAGVAP
                     KVRADLLDTTEESYDYVVNTNLRGVFFLTQAVARDMVAAKKADPAFNAAIINITSISA
                     TVISINRGEYCIAKAGLSMLTQLFAVRLGPDGIPVYEVRPGVIKTDMTSGVTAKYDKL
                     IAEGLCVQPRWGFPDDVGRAVASLARGDMPYSTGQVIMVDGGLTLPRL"
     gene            complement(4548..>4749)
                     /locus_tag="VEA63_05900"
     CDS             complement(4548..>4749)
                     /locus_tag="VEA63_05900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HYD83561.1"
                     /translation="FDGWTRVPDQGVYDNAFKVQWELFLKHVVTDSPFPWSLHEGAKG
                     VQLAELGLASWAKRAWVDVPKL"
BASE COUNT          909 a         1170 c         1805 g          865 t
ORIGIN      
        1 gggtgaaatg agttgggctg gcgaacgcgg aagtgggtcg catcagtagg tcgacgttcg
       61 tttaggagga aaacggaccg caaagggatt ttttttgatg aagcctgtcg aagtggcggg
      121 cgtccgctcg actagaggag aacaccacga acgattatga aatatgcatt gttgatttat
      181 ttggacgaaa agcaattcac ggcgctgccg aaagaagagc agaaccgggt tcaccaggag
      241 tgcggtgcgt ggcacgagga gttggtgaaa agcggacaca gcgtgggttg caaaggactg
      301 caagggacgg cgacggcgac gaccttgcgc gagcgagacg gaaaagtggc gatcatggac
      361 gggccgtttg cggagacgaa ggagatgctg ggcgggttgg aaacgctcga gtgcagggat
      421 ctcgacgagg cgctggggat cgcgaagcgg tttcccgggc tgcgcggcgg ccggtgtgcg
      481 gtggaggttc ggccggaggt gccgggagga acgtgcgagg catgaggtgc gaccgcggcg
      541 cagcgtgggc gaatggcgaa cgactcggcg agggcgtcgg gtttcgctgc ggatgaaatg
      601 agcgtcgagg agatttatcg gcgcgagtat gggcgtatcc tggcgagttt gattcgcgtg
      661 gtgcgggatt tcacgctggc ggaggatgcg ttgcaggagg cgttcgcggc ggcgcacgaa
      721 cggtgggcga aggaaggctg gccgcgaaat ccggtggggt ggttgatgac gacggcgcgg
      781 tggcgggcga tcgacgagat ccggcgacgg gcgctggcgg agaaccgacg ggaggagatc
      841 gcgctgatca gcggaccgca ggaggaagca ccgatgccga tcgacgagct gcgtttgatt
      901 ttcacgtgtt gtcatccggc gctggcgccg gacgcgcgcg tggcgttgac cttgcggacg
      961 gtgagcgggt tgacgacgga ggagatcgcg cgggcttttc tcgtgccggt gccgacgatg
     1021 gcgcagcggc tggtgcgggc gaagacgaag atcaagctgg cgggaatccc gtatctggtg
     1081 ccggcggacg atgagctgtc ggagcggttg gcggcggtgc tcgcgacgat ctacctggtg
     1141 tttaacgaag gttacgcggc gagcacggga gagaatctgg tgcgcgggga gttgtgcacg
     1201 gcggcgattc gcgtggggag agacttggtg gcgttgctgc cgcaggaacg cgaggcggcg
     1261 gggctgctgg cgttgatgtt gttgagcgat gcgcgacggg cggcgcgcac ggatgcggcg
     1321 ggcgaattgg tgttgttgga ggagcaggac cggacgcagt gggatcgcgc gaagatcgat
     1381 gaaggctgcg cgctggcgga gcgggcgttg ggcggcgcgt cacagccggg cggatacgcg
     1441 gtgcaggcgg cgatcgcggc cgtgcatgcg cgggcgaaaa cggcgggcga aacggattgg
     1501 agcgggattg cgcggtggta tgggttgctc gcgcggatcg cgccgtcgcc ggtggtggaa
     1561 ttgaatcgag cggtggcgat cgcgatggcg gaggggctcg agaaaggatt ggcgctgatg
     1621 gagcgaatcg acatgccggg ctactatttg gtgccggcgg cgcgggcgga cttgttgcga
     1681 cgattggggc ggaacgcgga ggcggccgcg gcgtatcggg aggcgctcgc gctggcgacg
     1741 aacgaagcgg agcggcggtt tttgatgaag cggatcgcgg aggtcgcgcg cgcggcggaa
     1801 tagcgcgcta tttctcgtcg acgaattcga gcagatcgcc gggctggcaa cgcagttcgc
     1861 ggcaaagcgc ctcaagcgtg ctaaagcgaa tggctttggc cttgttggtt ttgaggatcg
     1921 agagattcgc gagcgtgaga tccacgcgct cggcgagttc agtgaggctg atgccgcgct
     1981 tcgccatgac gacgtcgagg ttgagttgaa taggcatggc tcagaccgtg aggtcgtttt
     2041 cggttttgag ggtgaggccc tggcggaaag cctcgctcac cacgagcacg acgagaccgg
     2101 tgacgaggcc gccccactgc gggaactgcc caccgaggct gaaacgcaga gcgccaggtg
     2161 atgaggcgaa gccggtcgcc gtgacgtggt gcgcaaggta gtcgttcatc acgtgcgcgc
     2221 cccaccagcc cacgatgagc gtgacaaggc tgtaggcgat caatagcccg ccgatgccgc
     2281 ggaccaatcg gagattggtt tcggtgaaga cctgcccgcg ttcgatgttg gcgcagacgg
     2341 cgcggagtga gccgaagagc caccaggaga aaacgacgaa cgcgatcatc gcgggtagaa
     2401 tggtccagcg gacggcggaa acgagcgcgg cgtcgccggt cagcagatcc gcttggagcg
     2461 ggccgttgag atcgccgagg atcaacgcac caggcccggc ggcggtcgaa accaaggtcg
     2521 gcggctgagc cgattcggtg gctgtgactt cgccgacggt gacgatcagc ttcggatcat
     2581 cgacgaaacg tttttggatc caggcgttga acgtaagcgt gaaaaaccaa aacgcggcca
     2641 ggaggacgac gacgaggcga agagcgccga acagtgcgcg aagattaccg gggaggttgg
     2701 tgaagagttt cattggggag gccaattgcg gcttattgat attcgataaa catgcttggc
     2761 gtttcgtcaa cgtatttttt atcgaatatc gataattcga tcgaacggct caggggcgct
     2821 gttgcgaacc ttgttcgcgc ctacagcgtt ttcagaaaaa ccatcgccgt tcagatggag
     2881 ggccggcctc cgtgccggcc gcggacgaca cggaggtcgt ccctccagaa atggccatag
     2941 ctttgttgaa agcgctcgag gtgccgaaga gctggccgtc gcagcgagag cggataccga
     3001 tgtggttata aaccgacgaa ccggtgtcgt cttggcgggc ggcgttatcg gacttcgctc
     3061 atcagaccga cgaggttgtc gtcggggtcg cggaggaagg ccatccaaag gtcgtggtcg
     3121 ggcattttcg cggtgagctg cggagggcgt tcgttctttg cgcctcgggc gatgagggcc
     3181 gcgtgcgtgg cggggagatc gtcgaccttg aaatagagcg tggagttttt gccgacttca
     3241 ccgtggcctt gcggcgtggt gagcatcagg cggacagagc cggcggcgag gaaggcaaga
     3301 ttcggcccgg ctgcgaaaag gaagcggagg ccgagaacgt cgcgataaaa ggcggtggaa
     3361 acggagacgt cgcgaacggt gatcgcgatt tggccgatgt ctgaaagggc aaccggcata
     3421 caggaaagct ccaacatcca agcaccaagc tccagtgaat attcaaactc caagctccaa
     3481 ttgaggtttg gtgtttggca gttctctgga gcttggatct tggtgcttgg agcttccctg
     3541 ccgcgctccc aaggcagaaa tgcaggacgg cgctacagtc gcggcagggt cagtccaccg
     3601 tcgaccatga tgacttggcc ggtggaatac ggcatgtctc cgcgggcgag ggaggcgacg
     3661 gcgcggccga cgtcgtcggg gaaaccccag cgcggttgga cgcagaggcc ctcggcgatg
     3721 agcttgtcgt atttcgcggt cacgccggag gtcatgtcgg ttttaatcac gccggggcgg
     3781 acttcgtaaa cgggaatgcc gtcggggccg agacggacgg cgaagagctg cgtgagcatg
     3841 ctgaggccgg ctttcgcgat gcagtattcg ccgcggttga tgctgatgac ggtcgcggaa
     3901 atcgaggtga tgttgatgat cgcggcgttg aacgcggggt cggctttctt tgcggcgacc
     3961 atgtcgcggg cgacggcttg cgtgaggaag aacacgccgc ggagattcgt gttcaccacg
     4021 tagtcgtaac tttcctcggt cgtatccaga aggtcagcac ggaccttcgg ggcgacgccg
     4081 gcgttgttga cgagcaagct aagagctgag agcccagagc cgggagcttg accagcggcg
     4141 acgaagtcgc ggcaggtttt gaggatcgag gcgcggcctt cggcgctacc gacatcgcct
     4201 tgggcgtagg cgacacggac gccgtgcgcg cggagttgcg ccagcggttc ggccacggcg
     4261 gactcggggc ggacgccgtt gatgacgagg tcccattttt cggcggcgag tttttcggcg
     4321 atgccgaagc cgatgccgcg ggagccgccg gtcacgagcg ctactttttt cataagggtg
     4381 aggctgaatg tttcgtgaaa gctccaagca ccaacatcca agctccaatg aagcaccaat
     4441 cttcaatgct cggaaattgg atgtttggtg tttggacatt cactggagct tggatgttgg
     4501 tgcttggagc ttctgcgtgg tgcttggagc tttgagctgg gccgtattca gagcttgggg
     4561 acgtcgaccc acgcgcgctt cgcccaactg gcgagtccga gttcggcgag ttggacgcct
     4621 ttggcgcctt cgtggagaga ccaggggaac ggcgaatcgg tgacgacgtg tttcaggaag
     4681 agctcccact ggactttgaa ggcgttgtcg taaacgcctt ggtcggggac gcgcgtccag
     4741 ccgtcgaag
//
LOCUS       DASPLT010000568         2686 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_272726, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000568 DASPLT010000000
VERSION     DASPLT010000568.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 2686)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2686)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2686
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_272726"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(286..1998)
                     /locus_tag="VEA63_05905"
     CDS             complement(286..1998)
                     /locus_tag="VEA63_05905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HYD83562.1"
                     /translation="MNLIDWIVLLGTMLGIAAYGTWRTRHTDNLNTYLKGNRTTKWGT
                     IGLSVMATQASTITYLSLPGQAYENGIAFIQNYFGLPLALILVCAVFLPIYRKLGVYT
                     AYEYLGKRFDQKTRLLGAGLFLLQRGIQAGITIYAPAIILSTVLGWRLDLTIIFTGLL
                     AIVYTVTGGSAAVNLTQKWQMAVIFGGMITAFVVLLAKLPPDAAHIAGALGKLEAVDY
                     TPDLKQRYTFWSGILGGFFLSLSYFGTDQSQVQRYIGGAALREGRLGLMFNAVMKIPM
                     QFFIVMLGALLFVFYQFQPKTPIIFNQTQWQRQIDGPQGDAFRAFEQQHAAAHLERQE
                     KIRAWAAVRDSGDVAAETAARSAMLEAQAAANAVRLAARDALQAADPAAKKTRDSDYV
                     FITFILTQLPHGAIGLLIAVMFASALSSKAGELNALGTTSTIDLWRHFRPLAEHDEPR
                     NVRNAKWFTAAWGLFAISFALFVSFAENLIEALNIVASIFYPALLGVFIVAFFLKKVG
                     GSAVFWAAIAAQAVVIGIYIYGKMNPVHEIGYLWLNPIGCAACVLFSLILQALFRSPK
                     SNPS"
     gene            complement(1995..2168)
                     /locus_tag="VEA63_05910"
     CDS             complement(1995..2168)
                     /locus_tag="VEA63_05910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD83563.1"
                     /translation="MSARPSSSPPPPASADPGDDERPGVPLFQTWRGVYAFVFASFLL
                     VVLLLTIFTHVYA"
     gene            complement(2303..>2686)
                     /locus_tag="VEA63_05915"
     CDS             complement(2303..>2686)
                     /locus_tag="VEA63_05915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD83564.1"
                     /translation="IYVPLGPYPLSIQGPAPRHRVTDETAPIAILAPDHPALNTPNKI
                     TASDFEGWLQERGAYFPSSWDEAHYTSILALSDPGEPPLTSGLLVARHGSGYFVYTGL
                     SFFRQLPGGVPGAYRLFANLVSLGK"
BASE COUNT          537 a          629 c         1034 g          486 t
ORIGIN      
        1 cggatgaggg ggctaagatt ttttaaccac gaatggacac gaagggacac gaattcaaac
       61 cgggcaattc gtgtgaattg gtggttgaag cggctaggga gctttttgaa cgcgcttcaa
      121 aggaggagga attttttaac cacggatgga cacggatgaa cacggattgg gaagagacgg
      181 gagaggcggt ggatttgatc cgtgtttatc tgtgttcatc cgtggttgag agagatcggg
      241 gccgatctga tcggtccatt cgtgtgaatt cgtgtccatt cgtggttaag acggattgga
      301 tttgggcgaa cggaaaaggg cttggaggat gaggctgaag aggacgcagg cggcgcagcc
      361 gatggggttc agccagagat agccgatttc gtggactggg ttcatcttgc cgtaaatgta
      421 gatgccgatg acgacggctt gcgcggcgat cgcggcccag aagacggcgg agccgccgac
      481 ttttttcagg aagaacgcga cgatgaacac gccgaggagc gcgggataaa agatcgaggc
      541 gacgatgttg agcgcttcga tgaggttttc ggcgaagcta acgaagagcg cgaagctgat
      601 cgcgaagagg ccccacgcgg cggtgaacca ttttgcgttg cggacgtttc gcggttcgtc
      661 gtgttcggcg agcgggcgga agtggcgcca aaggtcgatc gtgctggtgg tgccgagcgc
      721 gttgagctcg ccggctttcg aggagagcgc ggaggcgaac atcacggcga tgagcaggcc
      781 aatggcgccg tgcgggagtt gcgtgaggat gaaggtgatg aacacgtagt cggaatcgcg
      841 cgtttttttt gcggcgggat cggcggcttg cagggcgtca cgcgcggcga ggcggacggc
      901 gttggcggcg gcttgcgctt cgagcatcgc actgcgcgcc gcggtttcgg cggcgacgtc
      961 accgctgtcg cggacggcgg cccacgcgcg gattttttcc tgacgctcga ggtgggcggc
     1021 ggcgtgctgt tgttcgaagg cgcggaaggc gtcgccttgc gggccgtcga tctggcgctg
     1081 ccattgcgtt tggttgaaga tgatgggcgt cttcggctgg aactggtaga acacgaagag
     1141 cagggcgccg agcatcacga tgaagaactg catcgggatt ttcatgacgg cgttgaacat
     1201 gaggccgagt cggccttcgc gcagcgcggc gccgccgatg tagcgttgga cctgcgattg
     1261 gtcggtgccg aagtaggaga gcgacaggaa aaagccgccg aggatgccgc tccaaaacgt
     1321 gtagcgctgc ttgaggtcgg gcgtgtagtc gacggcttcg agcttgccca aggcgccggc
     1381 gatgtgcgcg gcgtccggcg ggagcttggc gagcaacacg acgaacgcgg tgatcatgcc
     1441 gccgaagatg acggccatct gccatttttg cgtgaggttc acggcggcgc tgccgccggt
     1501 cacggtgtag acgatcgcga gcaggccagt gaaaatgatc gtgagatcga gtcgccagcc
     1561 gaggacggtg gagaggatga tcgccggtgc gtagatagta atgccggctt ggataccgcg
     1621 ttggaggaga aacaggccgg cgccgagaag gcgggttttc tggtcgaagc gttttccgag
     1681 gtattcgtag gcggtataca cgccgagctt gcggtagatc ggaaggaaga cggcgcaaac
     1741 gaggatgagc gcgagcggaa ggccgaaata attttggatg aacgcgattc cattttcgta
     1801 ggcctggccg ggcaaggaga ggtaagtgat cgtgctcgct tgcgtggcca tcacggagag
     1861 gccgatggtg ccccatttcg tggtgcggtt gcccttgagg taggtgttga ggttgtcggt
     1921 gtgacgcgtg cgccaggtgc cgtaagcggc aatgccgagc atcgtcccga gcagcacgat
     1981 ccaatcgatc aggttcatgc gtagacgtgc gtgaagatcg tgagcaggag gaccacgagg
     2041 aggaagctcg cgaacacgaa cgcgtagacg ccgcgccagg tttggaaaag cgggacgccg
     2101 ggtcgctcgt cgtcgccggg atcggcggat gcgggcgggg gcggtgaaga cgaagggcgg
     2161 gcagacatcg gcgaaggaga aaggagatgc ggaggtgaga aggcggatga gcgtcgatgc
     2221 ttacacccgc ctttcggtta cgtcgctagc gagagaggag gcgtggggcg acagaccgtg
     2281 tggtgagtgc gagcatgccg cttcattttc cgagcgagac gaggttcgcg aagaggcgat
     2341 aggcgccggg gacgccgccg ggaagctggc gaaaaaacga gagaccggtg tagacgaaat
     2401 agccggagcc gtggcgggcg acgagcaggc cgctggtgag cggcggttcg ccgggatcgc
     2461 tcaacgcgag gatcgaggtg tagtgggcct cgtcccagct actgggaaaa tacgcgccgc
     2521 gctcctggag ccagccctcg aaatcggagg cggtgatttt gttcggcgtg ttgagcgcgg
     2581 gatgatcggg cgcgagaatg gcgatgggcg cggtctcgtc ggtgacgcgg tggcggggcg
     2641 cgggaccctg aatcgaaagc ggataggggc cgagcgggac gtagat
//
LOCUS       DASPLT010000569         1284 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_89363, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000569 DASPLT010000000
VERSION     DASPLT010000569.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1284)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1284)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1284
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_89363"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1083
                     /locus_tag="VEA63_05920"
     CDS             <1..1083
                     /locus_tag="VEA63_05920"
                     /inference="COORDINATES: protein motif:HMM:NF037878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD83565.1"
                     /translation="SFQLYYRGALAIVSGLYRKNGLTDGYGNDYTKNYYWRSIAHNTL
                     LVHNPAEEFVETYGNDGGQRVPNQRREARTLDMLLDPENGYRTGEVLAHGFGPEAPMP
                     DYTLLQGDISAAYSEKVKSATRSFVFLNLRNSVVPAALITLDRVVSAEPDFRKYWLLH
                     TMEEPTIDGTSASVERTTHGDSGRLNLDVLLPGPDNTLLEKVGGPGKEYWVFGKNHPN
                     DVDPATMAKGTIEAGAWRLELSPKTPATEDVFLAVMQVTDRETGERWPARRIDAGERA
                     GCVLTGQGNAWVVLMRKDSTRSAQPVEFSFEGEGRARVLVTDLAPGRWQATRTGGGEQ
                     TIVEVGADSGAAWFEGSAGTWTLRVL"
BASE COUNT          247 a          376 c          440 g          221 t
ORIGIN      
        1 tcgttccagc tctattatcg cggcgcgctg gcgatcgtct cgggacttta tcgcaagaac
       61 ggtctgactg acggctacgg caacgactac acgaagaatt actattggcg gtcgatcgcg
      121 cacaacacgc tgctcgtcca caatcccgcg gaggagttcg tcgagactta tggcaacgat
      181 ggcggtcagc gcgtgccgaa ccagcgtcgc gaggcgcgca cgcttgacat gctgctcgac
      241 ccggaaaacg gctatcgcac cggcgaggtg ctcgcacatg gcttcgggcc tgaggcgccc
      301 atgccggatt acacgctgct gcagggcgac atttccgcgg cttacagcga gaaagtgaaa
      361 agcgcgacgc gttcgttcgt gtttctcaac ctgcgcaatt ccgtcgtgcc ggcggcgctg
      421 atcacgctgg accgggtggt gagcgccgaa ccggatttcc gaaaatactg gctgctgcat
      481 acgatggagg agccgacgat tgacggcact tcggcgagcg tggagcgcac gacgcatggc
      541 gacagcggac ggctgaacct cgacgtccta ttgcccgggc cggataacac gctgctcgaa
      601 aaagtcggag gaccggggaa ggagtattgg gtatttggca agaaccaccc caacgacgtc
      661 gatcccgcca cgatggcgaa aggcacgatc gaagcgggcg cgtggcggct ggaactttcg
      721 ccaaaaacgc cggcgaccga ggatgtgttt ctcgcggtga tgcaggtgac ggatcgcgaa
      781 accggtgagc gctggccggc gcggcgcatc gatgcgggtg aacgcgccgg ctgcgtgctc
      841 acagggcagg ggaacgcgtg ggtggtgttg atgcggaagg acagcacgcg ctccgcgcag
      901 ccggtggagt tttcgttcga aggcgaaggc cgcgctcgcg tgctggtgac cgatctcgcg
      961 cccggccgct ggcaggcgac gcgaaccggc ggaggcgaac aaacgatcgt cgaggtcggc
     1021 gccgattcgg gcgcggcgtg gttcgaaggc tccgccggca cgtggacgct ccgcgtgctc
     1081 tagggttatt ttgcaagcga acgctgctcg acccggtgaa catcggacct caccgcacgc
     1141 gtcgggctat agcggattaa ataagatcat cggccgaggt aggagcctgc ttgcaggcga
     1201 tctcgccgtg cgaacggcca acatcgctgc actgcgggtg tccgcagatg tctttaccga
     1261 ttagcgattc cgccgttcgc gagg
//
LOCUS       DASPLT010000570         1117 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_211807, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000570 DASPLT010000000
VERSION     DASPLT010000570.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1117)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1117)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1117
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_211807"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..946)
                     /gene="hisC"
                     /locus_tag="VEA63_05925"
     CDS             complement(<1..946)
                     /gene="hisC"
                     /locus_tag="VEA63_05925"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HYD83566.1"
                     /translation="MNFQSLVKPAVLSQPVYEPGKPIEDVAREIGIDPATIIKLASNE
                     NPFGPSPRAVAAAKAALEHGEVYPDGGCIALREKLAAARGLAPDQIVVGNGSNEIIEL
                     LGHALLGPGDEVVMASPAFVVYKLVTLLFGAKPIEVPLREHRHDLEALAAAITPRTKL
                     VFVCSPNNPTGTANTEAELRAFARAMPPHVVTVFDEAYAEYVEGPPDLRPLIVEGLKV
                     VCLRTFSKIYGLASLRVGYGYAAAEFASLLQRVRQPFNVNAIAQAAARAALDDTEFAE
                     RCARENRAGMRQLETGFKALGLELVPSVANFLLVRVGDG"
BASE COUNT          203 a          402 c          321 g          191 t
ORIGIN      
        1 cgccgtcgcc tacccgcacg agcaggaaat tggcgacgct cgggacaagc tccaacccga
       61 gcgctttgaa acccgtctcg agctgccgca ttcccgcacg attctcccgc gcgcaccgct
      121 cggcaaactc ggtgtcgtcg agcgccgcac gggccgccgc ctgcgcgatc gcattcacat
      181 tgaaaggctg ccgcacgcgc tgcagcagcg aggcgaactc cgccgcggcg tagccgtatc
      241 caacgcgcag cgacgccagc ccgtagatct tcgagaatgt ccgcaggcaa accaccttca
      301 ggccttccac gatcaacggc cgcagatccg gcggaccctc cacgtattcc gcatacgctt
      361 catcgaaaac cgtcaccacg tgcggcggca tggcccgcgc aaaggcccgt aattccgcct
      421 ccgtgttcgc tgtgcccgtg ggattattcg ggctgcagac gaacaccagt tttgtccgcg
      481 gcgtgatcgc cgcagcgagc gcctccaaat catgccggtg ctcgcgcagc ggcacttcga
      541 tcggcttcgc gccgaacagc agggtgacca gcttgtagac cacgaacgcc ggactcgcca
      601 tcaccacctc atctcccggc ccgagcagag catggccgag cagttcgata atctcgttcg
      661 atccgttgcc gactacgatt tggtccggag caagcccgcg agcggcagca agcttctcgc
      721 gcaacgcaat gcatccgcca tccggataca cttcaccgtg ctccagcgcc gccttggccg
      781 ccgccaccgc gcgcggtgac ggtccaaacg ggttttcatt ggacgcgagt ttgatgatcg
      841 tcgccggatc aatcccgatt tcccgcgcga catcctcgat gggcttgccg ggttcgtaga
      901 ccggctgcga cagcacggcg ggtttcacga gcgactgaaa gttcatgctc ttaggaatga
      961 acagccgggc gcgcgaatga aagtgcgttt ctcggcccgc cagcaccgcg ggtgctgctt
     1021 ttccgctcgg agcggaaact cccacctttc ctcaacgccg ccggctcggc acgcgcacga
     1081 ctttcatccc cgccgcgtga gcagcggcga tcccggg
//
LOCUS       DASPLT010000571         1532 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_89397, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000571 DASPLT010000000
VERSION     DASPLT010000571.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1532)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1532)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1532
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_89397"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(68..997)
                     /locus_tag="VEA63_05930"
     CDS             complement(68..997)
                     /locus_tag="VEA63_05930"
                     /inference="COORDINATES: protein motif:HMM:NF024602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4038 domain-containing protein"
                     /protein_id="HYD83567.1"
                     /translation="MIARYGAFNLVFSGIHLDWIPKDYSLTADEFNAALQYHLKTYGP
                     LPFEQPYTALIDSSTLKAFGHGEKAPWLDMHTVGNKPRNHAIYGSLEDIFRLEPAYPA
                     ANLEPYYTGWNHMMNRPGGETPAENSPRDNYFARAMMYGSVLSGGLAGHVHGTAAYDL
                     TTTGEPAGWRPHIWTALRYESGAQMRHLKNFVLSEGDRYQQLELASQDVSTRRKTNPL
                     DEGLDGWSFLMRTAARDFALAYFENKAEPPQLHGFTPNATYTWTWFNPRTGEWLPSQT
                     ADADPSGTLMTPAFPTDRDNTSLADWAARISQR"
     gene            complement(994..>1532)
                     /locus_tag="VEA63_05935"
     CDS             complement(994..>1532)
                     /locus_tag="VEA63_05935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD83568.1"
                     /translation="YRGARAAADYVPGPGISFEEAVAWRKRQGFNSVSFIAAFPNWAA
                     DHRGATFANKDGIYLRNAWEKFGHWAPNAKVSTADGATTTAKDMHDEHGNRPFEVFHD
                     REGLANFDRIVPAYFRSLDRKMKHLADEGFVPFFETIRRDNAPSWKAYFDFNPSYARF
                     VQYLIARYGAFNLVLSSI"
BASE COUNT          266 a          373 c          585 g          308 t
ORIGIN      
        1 tttgtcatct attgtatgac aaacgtgcga gaggtcggtc ggtcgctgtg gttttagccg
       61 aagggtttca gcgctggctg attcttgcgg cccagtcggc gagggacgtg ttgtcgcgat
      121 cagtggggaa ggcaggcgtc atgagcgtgc cgctggggtc ggcgtcggcg gtttgcgagg
      181 ggagccattc gccggtgcga gggttgaacc aagtccacgt gtaagtggcg ttgggagtga
      241 agccatggag ctggggcggc tcggctttgt tttcgaagta ggcgagggcg aaatcgcgcg
      301 cggcggtgcg catcaggaag gaccagccgt cgaggccttc gtcgagcggg ttggttttgc
      361 gacgggtgga gacgtcttgc gaagcgagct cgagctgctg gtagcggtcg ccttcggaga
      421 ggacgaaatt tttcaggtgc cgcatctgcg cgccggattc gtagcgaagc gccgtccaga
      481 tgtgcggacg ccagccggcg ggttcgccgg tggtcgtgag atcgtaggcg gcggtgccgt
      541 ggacgtggcc ggcgaggccg ccggagagga cggagccata catcatcgcg cgcgcgaaat
      601 aattatcgcg cggggaattc tccgcgggcg tttcgccgcc gggccggttc atcatgtgat
      661 tccagccggt gtaatacggc tcgaggttcg cggcgggata agcgggctcg aggcggaaga
      721 tgtcttcgag ggaaccgtag atcgcgtgat tgcgcggctt gtttccgacg gtgtgcatgt
      781 cgagccacgg ggctttttcg ccgtgaccga aggccttgag cgtggagctg tcgatcagcg
      841 cggtgtaggg ctgctcgaag ggcaacgggc cgtaggtttt taaatgatac tgaagcgcgg
      901 cattgaactc gtcggcggtg agcgagtagt ccttcgggat ccagtcgagg tggataccgc
      961 tgaacacgag gttgaacgcg ccgtatcgag cgatcaaata ctggacaaca cgaggttgaa
     1021 cgcgccgtat cgagcgatca aatactggac aaagcgcgcg taggatgggt tgaagtcgaa
     1081 gtaggctttc caggagggag cgttgtcgcg gcggatggtc tcgaagaacg gcacgaagcc
     1141 ttcgtcggcg aggtgcttca tcttgcgatc gagcgagcgg aagtaggcgg gaacgatgcg
     1201 gtcgaaattc gcgaggcctt cgcggtcgtg gaagacttcg aacggacggt tgccatgttc
     1261 gtcatgcatg tccttcgcgg tggtcgtggc gccgtcggcg gtggagactt tggcgttggg
     1321 tgcccagtgg ccgaattttt cccaagcgtt gcggagatag atgccgtcct tgttggcgaa
     1381 cgtggcgccg cggtgatcgg cggcccagtt cgggaaggcg gcgatgaagg aaacggagtt
     1441 gaacccctgg cgtttgcgcc aggcgacggc ttcctcgaag ctgatgcccg gaccgggaac
     1501 gtagtcggcg gcggcgcgcg cgccgcgata gg
//
LOCUS       DASPLT010000572         1078 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_150148, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000572 DASPLT010000000
VERSION     DASPLT010000572.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1078)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1078)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1078
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_150148"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..356)
                     /locus_tag="VEA63_05940"
     CDS             complement(<1..356)
                     /locus_tag="VEA63_05940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007363072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HYD83569.1"
                     /translation="MTIEQHEDYMRCALDVARKGWGTTHPNPMVGAIIVEEERIVAEG
                     FHAQDGGPHAERLALLARGRPPREGAILYVTLEPCSTAGRTGACTDAIIASGIKHVVV
                     GATDPNPAHAGEGFEV"
     gene            complement(435..>1078)
                     /locus_tag="VEA63_05945"
     CDS             complement(435..>1078)
                     /locus_tag="VEA63_05945"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HYD83570.1"
                     /translation="RAMKLHVLPAGPIQTNAYLLTVAARGEAVLIDAPGDVWAVVEPI
                     LRAEKCKLVELWLTHGHWDHTQGAAEVVRATGARVLAHADDRVMIETPEAMERFMGER
                     MKLEAVKVDRWVKQGERIEILGTTVEVRHVPGHCPGNVLFYFAAAKAAFVGDALFAGS
                     IGRTDLPGGNFAQLEAAIRSQIYTLPEETIVYPGHGPDTTVEAERATNPYVSG"
BASE COUNT          193 a          441 c          254 g          190 t
ORIGIN      
        1 aacacctcga atccttcgcc cgcgtgcgcc ggatttggat ccgtcgcgcc cacgaccaca
       61 tgcttgatgc ccgacgcgat gatcgcatcc gtgcacgcgc ccgtccggcc cgcggtcgaa
      121 cacggttcga gtgtcacata caagatcgca ccttcccgcg gcggccgccc ccgcgccaac
      181 aacgccaacc gctccgcgtg cggaccgccg tcctgggcgt gaaacccctc cgcgacgatc
      241 cgttcctcct ccacaataat cgctcccacc atcggattcg ggtgggtcgt gccccagcct
      301 tttcgcgcta catcgagcgc gcaacgcatg tagtcctcgt gttgctctat cgtcatcgct
      361 ggtgccatcg atccagcgcg attcggcccg cggagtccac cacgttcatc gcttcctccc
      421 gcaataacag gagtttaacc actcacgtaa ggattggtcg cccgctccgc ctccaccgtc
      481 gtatcaggcc cgtgccccgg ataaacgatg gtttcctccg gcagcgtata gatctgcgaa
      541 cgaatcgccg cctccagttg cgcgaaattc ccgcccggca aatccgtgcg cccaatgctc
      601 cccgcgaaca gcgcatcgcc cacaaacgcc gccttcgccg ccgcgaaata gaacaacacg
      661 ttccccggac aatgccccgg cacatgacgc acctcgacgg tcgtccccaa aatctcgata
      721 cgctccccct gtttcaccca tcgatccacc ttcaccgcct cgagcttcat ccgctcgccc
      781 atgaaccgct ccatcgcttc cggcgtttcg atcatcaccc gatcgtccgc atgcgccagc
      841 acgcgcgccc ccgtcgcccg cacgacctcg gccgcgcctt gcgtatgatc ccaatgcccg
      901 tgcgtcagcc atagctccac cagcttgcac ttctccgccc gcaaaatcgg ctccaccacc
      961 gcccacacgt cgcccggcgc atcgatcaac accgcctcgc cccgcgccgc cacggtaagc
     1021 aaatacgcat tcgtctgaat cggcccggcg ggcagcacgt gaagtttcat cgcccgcc
//
LOCUS       DASPLT010000573         1943 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_191911, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000573 DASPLT010000000
VERSION     DASPLT010000573.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1943)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1943)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1943
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_191911"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..935
                     /gene="argF"
                     /locus_tag="VEA63_05950"
     CDS             <1..935
                     /gene="argF"
                     /locus_tag="VEA63_05950"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HYD83571.1"
                     /translation="KETDFAAHELPGLFALARSLKAGRGKGDQPKPLAGRTWAMIFSK
                     SSTRTRVSFEVGIHELGGNPLFLNRNDIQLGRGESIEDTAKVLSRFVHGLIVRTFDHS
                     DVERLASAGSVPVINALTDFLHPCQIFTDAFTAAERWAKGDDLVGSLKGRKIAFLGDT
                     ACNMAYSWVLGANLFGMEIALGGPKEFAPAKEFDAFLAKEGYAGGYTFTTDPMEAVRG
                     ADIVYTDVWVSMGKEEETAARIKQMRPYAVTAKVFGGAKPDALFMHCLPAHPGEEVDQ
                     TVLDNPRSIIFDEAENRLHMQKAILATLALSRKA"
     gene            1031..>1943
                     /locus_tag="VEA63_05955"
     CDS             1031..>1943
                     /locus_tag="VEA63_05955"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HYD83572.1"
                     /translation="MKIVLAYSGGLDTSVIVKWLKETYDAEIITFAADVGQEEELKGL
                     DKKAKKTGASKHYTLDLVEEFARDFIYPMIRANAIYEGQYYLGTSIARPLIAKAQVDI
                     AKKEKADTVAHGATGKGNDQCRFELTYMALAPDLQIVAPWKIEAYRELFPGRAEMIAY
                     CAQHKIPVEASLKKPYSMDRNLLHISYEAGILEDPWFDPTTKENKGMFKLSVSPEDAP
                     DKAEYVELDFVKGNCVAVNGKKLSPAGVLKALNKLGGKHGIGRVDLVENRFVGMKSRG
                     VYETPGGTILMHGHRQIESLTMDREVMH"
BASE COUNT          412 a          523 c          631 g          377 t
ORIGIN      
        1 tcaaagaaac cgactttgca gcccatgaac tgccgggttt gtttgcgctt gcgcggagtt
       61 tgaaggcggg gcgggggaag ggcgatcagc cgaagccgct cgcggggcgg acttgggcga
      121 tgattttctc caagtcgtcg acgcgcacgc gggtttcgtt cgaggtcggc atccacgagc
      181 tcggtgggaa tccgcttttt ctgaatcgca acgacatcca gctcggacgc ggtgagtcga
      241 tcgaggatac ggcgaaagtg ctgtcgcggt ttgtgcacgg actgatcgtg cggacgttcg
      301 atcacagcga tgtcgagcgg ctggcgagcg ccggcagcgt gccggtgatc aatgcgctga
      361 cggacttttt gcatccctgt cagattttca ctgatgcgtt tacggcggcg gagcggtggg
      421 cgaaaggcga cgatctcgtc ggttcgctga aggggcggaa gatcgccttc cttggcgaca
      481 cggcgtgcaa catggcttac tcgtgggtgc tcggcgcgaa tctttttgga atggagatcg
      541 cgctcggcgg gccgaaggaa ttcgcgccag cgaaggagtt cgacgcattt ctcgcgaagg
      601 aaggttatgc cggtggctac acgttcacga ctgatccgat ggaagctgtg cgcggcgcgg
      661 acatcgtcta cacggacgtg tgggtcagca tgggcaagga ggaggagacc gcggcgcgta
      721 tcaaacaaat gcggccctac gcggtgacgg caaaggtgtt cggcggcgcg aaaccggacg
      781 cgctgttcat gcactgcctg ccggcgcatc cgggcgagga ggtcgaccag acggtgctcg
      841 ataatccgcg ctcgatcatc ttcgacgagg cggaaaaccg gctgcacatg cagaaggcga
      901 ttcttgcgac gctggccttg tccagaaagg cctgaaaatt tgaaccacgg atgaacacgg
      961 atacacacgg atctggtttg ttcatcgaaa gccatcggag ttcattcgcg gtttcgcttc
     1021 tctttttccc atgaaaattg tcctcgcata ctcgggcggc ctcgacacgt ccgtcatcgt
     1081 caaatggctc aaggaaactt acgacgctga gatcatcacc ttcgcggccg acgtcggcca
     1141 agaggaagag ctgaagggtc tcgacaaaaa ggcgaagaag acgggcgcct cgaagcacta
     1201 cacgctcgat ctcgtggagg agttcgcgcg cgacttcatc tatccgatga tccgcgcgaa
     1261 cgcgatttac gaggggcagt attacctcgg cacgtcgatc gcgcggccgt tgatcgcgaa
     1321 ggcgcaggtc gacatcgcga agaaggaaaa agccgacacc gtcgcgcacg gcgccacggg
     1381 caagggcaat gaccagtgcc gtttcgagct gacctacatg gcgctcgcgc cggatttgca
     1441 gatcgtcgca ccgtggaaaa tcgaggcgta tcgtgagttg tttcccggac gcgcggagat
     1501 gatcgcgtat tgcgcccagc acaagatccc ggtcgaagct tcgttgaaga agccctattc
     1561 gatggatcgc aaccttctgc acatctccta cgaggcgggc attctcgagg atccgtggtt
     1621 cgatccgaca acgaaggaga acaaggggat gttcaaactc tccgtctcgc cggaagacgc
     1681 gccggataag gcggaatacg tcgaactcga tttcgtgaag ggcaactgcg tcgcggtgaa
     1741 cggcaaaaag ctctcacctg cgggcgtgtt gaaagcgctc aacaaacttg gcggcaagca
     1801 cggcatcggc cgcgtcgatc tcgtcgagaa tcgttttgtc ggcatgaagt cgcgcggcgt
     1861 ttatgaaacg ccgggcggca cgatcttgat gcacgggcat cggcagatcg aatcgttgac
     1921 gatggatcgc gaggtcatgc aca
//
LOCUS       DASPLT010000574         1045 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_89422, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000574 DASPLT010000000
VERSION     DASPLT010000574.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1045)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1045)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1045
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_89422"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..811
                     /locus_tag="VEA63_05960"
     CDS             <1..811
                     /locus_tag="VEA63_05960"
                     /inference="COORDINATES: protein motif:HMM:NF015648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HYD83573.1"
                     /translation="QLATLVGIAFSLTFLAEAKRVADDIVAEREAAALAASEASDPSA
                     VITPAGEPPHRNRRHWSGREHAIRDALKNPANPFGRLIERSPWWLFPLIAVIEFGGIL
                     LYLLQIPVTYALVRLEFESHWYIVTDRSLRIRSGLVSMQESTMSFANIQQVTVSQGPL
                     QRLLGIADVRVQSAGGGSGSVGEGHSRDSMHTGIFHGVDNATEVRDLILERLRLFRAA
                     GLGDPDDHHEAVAATAQLQSDAANAAQPALAAAQELLAEARALRAFLAAAR"
     gene            complement(908..>1045)
                     /locus_tag="VEA63_05965"
     CDS             complement(908..>1045)
                     /locus_tag="VEA63_05965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HYD83574.1"
                     /translation="LVIALIAGVLGFGVVAGTAATIAKICFVIFLVLFLVSLLRGRRA
                     P"
BASE COUNT          197 a          366 c          308 g          174 t
ORIGIN      
        1 ccagctggcg acgctcgtcg gtatcgcctt ttcccttaca tttcttgcgg aggccaaacg
       61 cgtcgccgac gatatcgtcg ccgagcgcga ggctgccgca ctcgccgcca gcgaggcgag
      121 cgacccctcc gccgtcatca cgcccgcagg cgaaccgccc caccgaaatc gccgccactg
      181 gtccggacgg gagcatgcga ttcgcgacgc gttgaaaaac cccgcaaacc cgttcggccg
      241 gctgatcgag cgttcaccgt ggtggctgtt tcccctcatc gccgtgatcg agttcggcgg
      301 tattctgctc tacttgctgc agattcccgt gacctacgcg ctcgtgcggc tcgagttcga
      361 atcgcactgg tatatcgtca ccgatcgcag cctgcgcatc cgttcgggcc tcgtttcgat
      421 gcaggaatcg accatgagtt ttgccaacat tcagcaagtc accgttagcc agggtccgct
      481 gcagcgactg ctcggcatcg ccgatgtgcg cgttcaatcg gcaggcggag ggagcggcag
      541 cgtgggcgaa ggccacagcc gcgattcgat gcacaccgga attttccacg gcgtcgacaa
      601 cgcgacggaa gtccgcgacc tgatcctgga gcgtctgcgc ctctttcgcg ccgcgggcct
      661 cggcgatccc gacgatcacc acgaagccgt tgccgcgacg gcccaacttc aatctgacgc
      721 cgcgaacgcc gctcaacccg cgctcgcggc cgcacaagaa ttgctcgccg aagcacgcgc
      781 cctccgcgcg tttctcgcag ccgcaaggta ggagcctgct tgcaggcgag tttggacgca
      841 tcacgcgcag tcgatgcctt atatcgcctg caagcaggct cctaccttcg aaccgcaacg
      901 accggcatca aggcgcgcgc cggccgcgta acagcgagac gaggaacagc accaggaaga
      961 tcacaaagca gatcttcgcg atcgtcgcag ccgtgccggc gaccacgccg aagccgagaa
     1021 cgcccgcaat cagggcgatg acaag
//
LOCUS       DASPLT010000575         1235 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_231641, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000575 DASPLT010000000
VERSION     DASPLT010000575.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1235)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1235)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1235
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_231641"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1235
                     /locus_tag="VEA63_05970"
     CDS             <1..>1235
                     /locus_tag="VEA63_05970"
                     /inference="COORDINATES: protein motif:HMM:NF012722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HYD83575.1"
                     /translation="LVDAPSATWLTSPVGAVSSTNPGVKLLRFGDTAQPANAVVPASL
                     LNEIGEVSTLLEEGDGAARVVWIAGARGLLRCVLTDLPRETRPNSPMLRRVSPARPGA
                     SEEVRFEYSSPWFKSGEPMMYQTRLDGLTDAWSEFSAEPHTVFHRPPAGHFTFRVRAR
                     NADGLVGPESTHTFMIPKPWWLTPWMFGLYAIAAAGAVTTGVRWRLRTVQRRNAELEQ
                     IVTQRTAELRDAKGAAESANHAKSAFLANMSHELRTPLNAILGYAQILRKETGLSDKG
                     RHQLGIVGRNGEHLLQMINEVLDLAKIEAGKMTLHAADFPLARVVKTAADLLEQRAAE
                     KRLAFRLEFGAGLPRTVSTDEQKLRQVLLNLLGNAVKFTVYGEVVLSVARHDAHVRFE
                     VRDTGPGIAPDQIEAIFQP"
BASE COUNT          226 a          437 c          353 g          219 t
ORIGIN      
        1 ctcgtcgacg caccttccgc cacgtggctg acctcccccg tcggcgccgt ttcgtccaca
       61 aatccgggcg tcaaactcct gcgcttcggc gacaccgcgc aacctgcgaa cgccgtcgtg
      121 cctgcctcgt tgcttaacga aatcggcgag gtcagcacat tgctcgaaga aggcgacggt
      181 gccgcccgcg tcgtgtggat tgcaggcgcg cgagggctgt tgcgctgcgt gctcacggac
      241 ttgccacgtg aaacgcgccc gaattcgccc atgctacgac gcgtttcgcc cgctcgtccc
      301 ggcgcttcag aggaagttcg cttcgagtat tcgtcgccct ggttcaagtc aggcgagccg
      361 atgatgtatc agacccggct ggacggcctg accgatgcct ggtcggaatt ctccgccgaa
      421 ccacacaccg tctttcaccg cccaccggcg gggcatttca cgtttcgcgt tcgagcgcga
      481 aacgcggatg gactggttgg accggagagc acgcacacgt tcatgatccc caaaccctgg
      541 tggctcacac cgtggatgtt cggactctat gcgattgccg ccgctggtgc ggtgacgacc
      601 ggcgtgcgtt ggcgccttcg aaccgtgcag cgccgcaacg ccgagctcga gcaaatcgtg
      661 acccagcgca ccgccgagct ccgcgacgcc aaaggcgccg ccgaaagcgc caaccacgcg
      721 aagagcgctt ttctcgccaa catgagccac gagctccgca cgccgttgaa tgcaattctt
      781 ggatacgccc agattctccg caaggaaacc ggtctctccg acaaaggccg ccaccagctc
      841 ggcatcgtcg gccgcaacgg cgaacacctc ctccagatga tcaacgaggt gctcgacctc
      901 gccaagattg aggccggcaa gatgacgctt cacgccgccg attttcccct cgcgcgcgtc
      961 gtcaaaaccg ccgccgacct gctcgagcag cgcgccgcgg agaaacgcct cgctttccgc
     1021 ctcgaatttg gcgccggcct gccgcgcacg gtttccaccg acgaacaaaa attgcgccag
     1081 gtgctgctaa acctcctcgg caacgcagtg aagttcacgg tgtatggcga agtcgttctc
     1141 tccgtcgccc gccacgatgc tcacgttcgc ttcgaagtgc gcgataccgg gccgggcatc
     1201 gcgcccgacc agatcgaagc gatcttccag ccttt
//
LOCUS       DASPLT010000576         2085 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_108404, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000576 DASPLT010000000
VERSION     DASPLT010000576.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 2085)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2085)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2085
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_108404"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..226
                     /locus_tag="VEA63_05975"
     CDS             <1..226
                     /locus_tag="VEA63_05975"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HYD83576.1"
                     /translation="RDRVTRYRLVNHVDDFYSLAPGNDHLRPAQATPVRGLSLAGDYT
                     RQKYVATMEGAVVSGQLAAEAASADLRTAG"
     gene            317..1030
                     /locus_tag="VEA63_05980"
     CDS             317..1030
                     /locus_tag="VEA63_05980"
                     /inference="COORDINATES: protein motif:HMM:NF018580.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OBAP family protein"
                     /protein_id="HYD83577.1"
                     /translation="MMGLAVALGLAGIAMAAEGAHAEHRVPGEAKSGKTRMLEAGAEL
                     LQREAPLRRIHAHVCGFHFYNGEPGRQVRADHYCAHLNDEVRQCVIYDSDKEDARLIG
                     IEYIISERLFRELPEEEKKLWHSHRFEVKSGQLIAPGLPEVAENALMEELVTTYGKTW
                     HTWQVDRGDALPLGVPQLMMGFTADGQIDEALLRARDEDLRVSTMEKKAGRANIPVRE
                     IAPGADAWQRGEVVQLKLK"
     gene            complement(1154..>2085)
                     /locus_tag="VEA63_05985"
     CDS             complement(1154..>2085)
                     /locus_tag="VEA63_05985"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HYD83578.1"
                     /translation="RITRGKLQIDRQPVSLHAVLDDAISTVQADFVDKKIALHRHFDA
                     HRPVVVGDAVRLQQVFWNILKNAVKFTPAGGKVTIETRLDADNTRARVSITDTGIGLE
                     PAELERIFEAFVQGDHAGQRSRHFGGLGLGLAISKMLVDLHGGQISAASDGTGRGATF
                     TVELPLATADRVLDAPAPTPDRAERPQADRAMKRILLVDDHEATRNALAFLLQRRNFE
                     VLPAGSLTEALALAERGGIELIVSDIGLPDGSGYDLMERFGKVQGVKGIALTGYGTEQ
                     DLALSEQAGFAAHLTKPVRIEALDEALKTVLAG"
BASE COUNT          375 a          530 c          813 g          367 t
ORIGIN      
        1 gcgcgatcgc gtgacgcgtt accggctggt gaaccacgtg gacgattttt attcgctcgc
       61 gccagggaac gatcatctgc ggccggcgca ggcgacgccg gtgcgcgggc tgtcgctggc
      121 gggggattac acgcggcaga aatatgtcgc gacgatggaa ggcgcggtgg tgtcggggca
      181 actcgcggcg gaggcggcgt cggcggattt acgaacggcg ggttgaatcg aaaaactgaa
      241 tacaggagga cgcaccatga aaagcgaacg acgaaaatgg ggacggccgc aaagaggcac
      301 agaaggcgca aagagtatga tgggattggc ggtggcgctg gggttggcgg ggatcgcgat
      361 ggcggcggag ggggcgcatg cagagcatcg cgtgccgggc gaggctaaga gcggtaagac
      421 gcggatgctg gaagcggggg cggagctgtt gcagcgcgag gcgccattgc ggcggattca
      481 tgcgcacgtg tgcgggttcc atttctacaa cggcgaaccg gggcggcagg tgcgcgcgga
      541 tcattattgt gcgcatctga acgatgaggt gcggcagtgc gtgatctacg attccgacaa
      601 ggaagacgcg cggctgatcg ggatcgaata catcatcagc gaacggctgt ttcgcgagtt
      661 gccggaggag gaaaaaaagc tgtggcattc gcatcgcttc gaggtgaagt cggggcagtt
      721 gatcgcgccg gggctgccgg aggtggcgga gaacgcgttg atggaagagt tggtgacgac
      781 gtatggaaaa acgtggcaca cgtggcaggt ggatcgcggc gacgcgctgc cgctcggcgt
      841 gccgcagttg atgatgggct ttactgcgga cgggcagatc gacgaggcgt tgttgcgtgc
      901 gcgcgacgag gacttgcgcg tgagcacgat ggaaaagaaa gcgggtcgcg cgaatattcc
      961 ggtgcgcgag atcgcgcccg gcgcggatgc gtggcagcgc ggcgaggtcg tgcagttgaa
     1021 gctgaagtaa agcaccaagc tccaagcacc aaactccagt gaaacttcaa tccgccaagt
     1081 tccaattgct tccgtgcttt ccctgaggtt cggggcttct ttggagcttg gagcttccga
     1141 cgcggcggcg gcgttacccg gcgaggacgg ttttcagcgc ttcgtcgagg gcttcgatgc
     1201 ggacgggttt ggtgaggtga gcggcgaagc cggcttgttc gctcaacgcg aggtcctgct
     1261 cggtgccgta gccggtcaag gcgatgcctt tcacgccctg cacttttccg aagcgttcca
     1321 tcaagtcgta gccgctgccg tcgggcaggc cgatgtccga gacaatgagc tcgatgccgc
     1381 cgcgttcggc gagcgcgagc gcttcggtga gcgagccggc ggggagcacc tcgaaatttc
     1441 gacgctggag gaggaaggcg agggcgttgc gcgtcgcttc gtgatcgtcg acgaggagaa
     1501 tgcgtttcat cgcgcgatcg gcctgggggc gctcggcgcg atcgggagtg ggcgcgggtg
     1561 catcgaggac gcggtcggcg gtcgcgagcg ggagctcgac ggtgaacgtg gcgccgcggc
     1621 cggtgccatc gctggcggcg gagatttgtc cgccgtgcag gtcgacgagc attttcgaga
     1681 tggcgaggcc gaggccgagg ccgccgaagt ggcggctgcg ctgaccggcg tgatcgcctt
     1741 gcacgaaggc ttcgaagatc cgctcgagtt cggcaggctc gaggccgatg ccggtgtcgg
     1801 tgatcgaaac gcgggcgcgg gtgttgtcgg cgtcgaggcg tgtctcgatc gtgaccttgc
     1861 cgccggcggg agtgaacttg acggcgtttt tgaggatgtt ccaaaacacc tgttgaaggc
     1921 gcacggcatc gcccaccacg acggggcggt gtgcgtcgaa atggcggtga agcgcgatct
     1981 tcttgtcgac gaagtcggcc tgcacggtgg agatggcgtc gtccaacacg gcgtgaaggg
     2041 agacgggctg ccggtcgatt tggagtttgc cgcgggtgat gcggg
//
LOCUS       DASPLT010000577         1525 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_46435, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000577 DASPLT010000000
VERSION     DASPLT010000577.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1525)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1525)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1525
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_46435"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1016)
                     /locus_tag="VEA63_05990"
     CDS             complement(<1..1016)
                     /locus_tag="VEA63_05990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase family protein"
                     /protein_id="HYD83579.1"
                     /translation="MSADPNAAAAPAPAPSDFIRDIVAQHVAEQRYAKIVTRFPPEPN
                     GYLHIGHAKSICLNFGIARENDGQCNLRMDDTNPAKEDVEYVDSITADVRWLIDGWAD
                     HTLGFKSKGATPTKQLVNGREDYHLAPAPLSNLRSQISNPASSATAPASAEPFFASDY
                     FEQLYDYAVALIRKGKAYVDDLSPEDTDAYRGAPDKPGRDSPFRTRSIEENLDLFARM
                     RAGEFPNGARSLRAKIDMASPNIWLRDPLLYRIRHVPHHHAGDKWCVYPLYDFAHCLS
                     DYIEGVTHSICTLEFEVHRPLYDWILENLDLPRALPRQYEFAKLIPSYMIVSKRRLIQ
                     LVNE"
     gene            1310..>1525
                     /locus_tag="VEA63_05995"
     CDS             1310..>1525
                     /locus_tag="VEA63_05995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012377016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreE"
                     /protein_id="HYD83580.1"
                     /translation="MLNLVHFPVADPDPTLAEVALRVDRIVIAKRIWRGVAEDGVEFG
                     FELERPLKDGEVFWQTAKARYVVRQEAE"
BASE COUNT          272 a          328 c          606 g          319 t
ORIGIN      
        1 ttttcgttca cgagctgaat gagccgtcgc ttggagacga tcatgtagct cgggatgagt
       61 ttcgcgaact cgtattggcg gggaagagcg cgcgggaggt cgaggttttc gaggatccag
      121 tcgtagagcg ggcggtgaac ctcgaactcg agcgtgcaaa tgctgtgggt gacgccctcg
      181 atgtaatcgc tgaggcagtg ggcgaagtcg tagagtggat acacgcacca tttgtcgccg
      241 gcatggtgat gcgggacgtg gcggatgcgg tagaggagcg ggtcgcgcag ccagatgttg
      301 ggcgaggcca tgtcgatctt ggcgcggagc gagcgggccc cgttgggaaa ttcgccggcg
      361 cgcatgcggg cgaagagatc gaggttttct tcgatggaac gcgtgcggaa aggggaatcg
      421 cgaccaggct tgtcgggggc gccgcgatag gcgtcggtgt cttccggact gaggtcgtcg
      481 acgtaggctt tgcctttgcg aatgagcgcg acggcgtagt cgtagagctg ttcgaagtag
      541 tcggaggcga agaagggttc cgcggaggcg ggggcagtgg cggatgaggc gggatttgag
      601 atttgagatc tgagatttga gagcggggcg ggcgcgaggt ggtagtcttc gcggccgttg
      661 acgagctgtt tggtgggggt ggcccctttg gatttaaagc cgagcgtgtg atcggcccag
      721 ccgtcgatca gccagcggac gtcggcagtg atggaatcga catactcgac gtcttccttc
      781 gccggattgg tgtcgtccat gcggaggttg cactggccgt cgttttcgcg ggcgatgccg
      841 aagttgaggc aaatggattt cgcgtggccg atatggaggt agccgttggg ctcgggcgga
      901 aagcgcgtga cgatcttcgc gtagcgttgt tccgcgacgt gctgggcgac gatgtcacga
      961 atgaaatcgc tgggggcggg tgcgggagcg gcggcggcgt tgggatcggc agacatgagc
     1021 gaagagagtg aaggggcaat gtgcgcgtcg aaatggggtg aggcaacgat tggtttttgg
     1081 tgcagcgtca ggaccggaga aaacgcgcta tgcattcaga tgtcgcatat gcgacatcta
     1141 actgcacggt cggcggaagt tggactggag gcggttgtca gtgagcggcg gttgggggtt
     1201 acagaaagat gtcgcatctg cgacattccc gtgggcgggg tgaaatggag ggttgcgatg
     1261 ggggcggggg tgcgactcaa acggcaggct tcgtgctttt ccttacgtct tgctgaacct
     1321 cgttcacttt cctgttgccg atcccgatcc gacgctggcg gaggtcgcgt tgcgcgtgga
     1381 tcgaatcgtg atcgcaaaac ggatctggcg cggggtggcg gaggatggag tggagtttgg
     1441 gttcgagctc gaacgtccgc tgaaggatgg cgaggtgttt tggcagacgg cgaaggcgcg
     1501 ttatgtggtg cggcaggaag cggag
//
LOCUS       DASPLT010000578         1108 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_171604, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000578 DASPLT010000000
VERSION     DASPLT010000578.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1108)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1108)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1108
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_171604"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..182)
                     /locus_tag="VEA63_06000"
     CDS             complement(<1..182)
                     /locus_tag="VEA63_06000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD83581.1"
                     /translation="MHRLTLRLFLLFLSPLLAAASDASPFSVHLAWRDGAPRGQVEVT
                     PGRLDRIDILDTNGSVA"
     gene            complement(298..>1108)
                     /locus_tag="VEA63_06005"
     CDS             complement(298..>1108)
                     /locus_tag="VEA63_06005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD83582.1"
                     /translation="FVNSWMGANSSWGTDSLETPIVSSPTGFTATEDSLSEGYEVELI
                     ANPTPNWRIAVNASQTEAVRENVPGANFRAVAEFIDDAFQNTDVGLAPVWWPQNFEGA
                     RVIGPYAFNFRPDWLRVAALNGQSAGEIRKYRANVISSYDFTDGRLKGFGVGGGYRWE
                     DRSIIAYAPMKNEDGGYGVNLNAPFFAPREDSLDLWVSYSRKLTSKINWKIQLNVFGV
                     GEKDRLVPLAAGVDAARIGNVETPGMVVPMKATSFAIREGMSWQIRNTFEF"
BASE COUNT          225 a          319 c          371 g          193 t
ORIGIN      
        1 gcgaccgatc cgtttgtatc cagaatgtcg attcgatcca accggccggg tgtgacctcg
       61 acctggccgc gcggagcgcc atcgcgccag gcgaggtgca cggaaaacgg cgaggcatcg
      121 gacgcagcgg cgaggagtgg agaaagaaag agaagaaata ggcggagcgt gaggcggtgc
      181 atgatcgaga ggaaccgaag cgatgatgct cgtggagcag cggaggtgaa aaagaaaagc
      241 gccccggccg gtgaaggtcg gggcgctcga cgagaactga ccaagcacaa ctgacgatca
      301 gaactcgaac gtattgcgga tctgccagct catgccttcg cggatggcga agctggtggc
      361 cttcatcggg accaccatgc cgggcgtttc tacgttgccg atgcgcgcgg catcgacgcc
      421 ggcggcgagc ggaaccaggc ggtccttttc gccgacgccg aacacgttca actggatctt
      481 ccagttgatc ttgctcgtga gcttgcggct gtagctgacc cagaggtcga gcgagtcttc
      541 gcgcggcgcg aagaacggag cattgaggtt cacgccatag ccgccgtctt cgttcttcat
      601 cggagcgtag gcgatgatcg agcgatcttc ccaacgataa ccgccaccca cgccgaaccc
      661 tttcaggcga ccgtcggtga agtcgtagct gctgatcaca ttggcgcgat acttgcgaat
      721 ttcaccggcg gattggccgt tgagggccgc cacgcgcagc cagtcggggc ggaagttgaa
      781 cgcgtagggt ccgatgacgc gggcgccctc gaagttctgc ggccaccaca ccggggcgag
      841 accgacgtcg gtattttgga acgcatcgtc aatgaattcg gcgacagcgc ggaagttcgc
      901 ccccggcacg ttttcgcgga ccgcttcggt ttgcgaagca ttcacggcga tgcgccagtt
      961 cggggtcggg ttggcgatca actcgacttc gtagccctcg gacaacgagt cttccgtggc
     1021 agtgaacccg gtgggcgacg agacgatcgg cgtttccagg ctatcggtgc cccaggacga
     1081 gttggcgccc atccaggagt tcacgaac
//
LOCUS       DASPLT010000579         2355 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_171605, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000579 DASPLT010000000
VERSION     DASPLT010000579.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 2355)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2355)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2355
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_171605"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..253)
                     /locus_tag="VEA63_06010"
     CDS             complement(<1..253)
                     /locus_tag="VEA63_06010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD83583.1"
                     /translation="MALAPAARARLHATFACLGTLVLWWRFNGVLTTALVTLAAILAL
                     LAWFAPRHYALVQHLFDRCTHALLALITWTALGAVYFGVF"
     gene            422..>2355
                     /locus_tag="VEA63_06015"
     CDS             422..>2355
                     /locus_tag="VEA63_06015"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HYD83584.1"
                     /translation="MLWITPLCCRGVRTLGAVVLAAMIATELIADDGRPVPEWCDPMA
                     PLKAQPESTRRMVQRLGALEGKAKPFANRFHTVGAIVALRERIAQVSADEALPLKPRL
                     AEALLNDGQSVAALGEFESYERAVAAGGMTLKPEFEARLLMMKALCHLRIGEQENCLT
                     NHNADSCIFPIQGGGVHVRPEGSRAAVALLTQVLERYPERLDTVWLLNIAAMTLGEYP
                     AGVPARWRLAPEIFASEHELPRFRDVAAPLGLDVDDLSGGVVLDDFDNDGNLDLMMSA
                     FGFRGQLRFFYNRGDGTFTDLTDRAGLTGLTGGLNLIQADYDNDGFVDVFVLRGAWLD
                     NEGRFPNSLLRNNGDGTFTDVTEEAGVLSFHPTQTGVWFDYDGDGLIDLFVGNESTSA
                     ESVHPCELYRNNGDGTFTECAAENGVAVTGFVKGVAAGDYNRDGRPDLFLSVREAPNR
                     LLRNDGPADTRGRWRFTDVAPAAGVTEPLVSFPCWFWDFDNDGWEDLMVFGYKIRDVG
                     EVAADYLGRPHLAERARLYRNNGDGTFSDVTRATGLYRVVHAMGCNFGDLDNDGWLDF
                     YAGTGDPFLGTLIPNRMFRSDGGRRFQDVTTAGGFGQLQKGHAVAFGDLNGDGAQDVY
                     SVLGGAYPGDNYRNQLLANPG"
BASE COUNT          406 a          580 c          936 g          433 t
ORIGIN      
        1 tgaagacgcc gaagtaaact gcgccgagag cggtccaggt gatgagggcg aggagcgcgt
       61 gagtgcagcg atcgaagagg tgttggacga gtgcgtagtg acgcggcgcg aaccacgcga
      121 gcaacgcgag gatcgcggcg agcgtcacga gagcagtcgt aagcacaccg ttgaaacgcc
      181 accaaagaac gagcgtgccg aggcaggcga acgtggcgtg caggcgggcg cgagcggctg
      241 gggcgagggc catgcagcca gcgtgtgggg tggtgggaat gcgccaacga aaaaggcgga
      301 gcgcggcact cacacattgg cgggcgtttg agcccgacgg gaggtcgagc tccatcgggc
      361 gaggctgcat cgggcgagat tgacgcgggg aagggtgggc tcagttttca agcgtatcct
      421 catgctttgg atcaccccat tatgctgtcg cggtgtgcgc acgttgggtg cggtggtttt
      481 ggcggcgatg attgcgacgg agttgatcgc ggacgatggg cggccggttc cggagtggtg
      541 cgatccgatg gcgccgctca aggcgcagcc ggagagcacg cggcgaatgg tgcagcggct
      601 cggcgcgctc gaaggaaagg cgaagccgtt cgcgaatcga tttcacacgg tgggggcaat
      661 cgtcgcgctg cgcgagcgga tcgcacaggt gtcggcggac gaggcgctgc cgttgaagcc
      721 gcggctggcg gaggcgttgc tcaacgatgg gcagagtgtc gcggcgctgg gggagttcga
      781 gagttacgag cgggcggtgg cggccggcgg gatgacgctg aagccggagt tcgaggcgcg
      841 gctgttgatg atgaaggcgc tttgtcatct gcgaatcggc gagcaggaga attgtctgac
      901 gaaccacaat gccgactcgt gtatttttcc gattcaggga ggcggcgtgc atgtgcgtcc
      961 ggagggatcg cgggcggcgg tggcgttgtt gacgcaggtg ctggagcggt atccagaacg
     1021 cttggacacg gtgtggttgt tgaacatcgc ggcgatgacg ctcggcgaat atccggcggg
     1081 cgtgccggcg cgctggcggc tggcgccgga gatttttgcg tcggagcacg agctgccgcg
     1141 ttttcgcgac gtggccgcac cgctgggatt ggacgtggac gatttgtcgg gcggcgtggt
     1201 gctcgacgac ttcgacaacg acgggaatct ggacttgatg atgtcggcgt tcgggtttcg
     1261 cgggcagttg cggtttttct ataaccgcgg ggacggcacg ttcacggatt tgaccgatcg
     1321 cgcggggctg acgggactga cgggcgggct gaatctgatc caggcggatt acgacaacga
     1381 tggattcgtg gacgtgttcg tgttgcgggg cgcgtggctc gacaacgaag ggcgctttcc
     1441 gaattcgctg ctgcggaaca atggcgacgg aaccttcacc gacgtgacgg aggaggccgg
     1501 cgtgctgagt tttcatccga cgcagacggg cgtgtggttc gactacgacg gtgacgggct
     1561 gatcgacctt ttcgtgggta acgagtcgac gtcggcggag tccgtgcatc cgtgcgagtt
     1621 gtatcgaaac aatggcgacg ggacgttcac cgaatgtgcg gcggagaatg gcgtcgcggt
     1681 gaccggcttc gtgaaaggcg tggcggcggg cgactacaat cgcgacgggc ggccggattt
     1741 gtttttatcg gtgcgagagg cgccgaatcg gttgctgcgg aacgacgggc cggcggacac
     1801 gcggggtcgc tggaggttta cggacgtggc gccggcggcg ggggtgacgg agccgctcgt
     1861 gagttttccc tgctggttct gggatttcga caacgacggc tgggaggacc tgatggtctt
     1921 cggttacaag atccgcgacg tcggcgaggt ggcggcggat tatcttgggc ggccgcattt
     1981 ggccgagcgg gcgaggttgt atcggaataa tggcgacggg acgttcagcg acgtgacgcg
     2041 agcgacgggg ctttatcgcg tggtgcatgc gatgggctgc aacttcggcg atttggacaa
     2101 cgacggttgg ctggatttct atgcgggcac gggcgacccg tttctcggga cgctgattcc
     2161 gaaccggatg tttcggagcg acggcggcag gcgatttcag gacgtgacga cggcgggcgg
     2221 attcggccaa ctgcagaaag ggcacgcggt cgcgtttggc gatctcaacg gggacggtgc
     2281 gcaggacgtt tactcggtgc tgggtggcgc gtatccgggc gacaattacc gcaatcagct
     2341 gctggcgaac ccggg
//
LOCUS       DASPLT010000580         1731 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_108542, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000580 DASPLT010000000
VERSION     DASPLT010000580.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1731)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1731)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1731
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_108542"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..785)
                     /locus_tag="VEA63_06020"
     CDS             complement(<1..785)
                     /locus_tag="VEA63_06020"
                     /inference="COORDINATES: protein motif:HMM:NF016114.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding domain-containing protein"
                     /protein_id="HYD83585.1"
                     /translation="MQNKYSDDRLRLAARLYYIDGLGQADVAKFVKVSQAKVSRLLAL
                     ARERGIVRITVADYEPRQAELEAQLRAQLGLATVIVIKAIEELPSVDLRRSVGHFAST
                     AVEALIQPRDIVAIAGGRTIHELVHHLSEVRNKALTVIQAMGSVDSNVSAFDAQEVGR
                     VMAQRLGGVFLSLNAPAYIPDKRTRDALLKLAQVNEVHEQLSRATFALVGLGTLDNSV
                     FVERGVLEPADVAELRAAGAVGEVCGRFFDQDGEECDTAWRDRV"
     gene            1122..>1731
                     /locus_tag="VEA63_06025"
     CDS             1122..>1731
                     /locus_tag="VEA63_06025"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HYD83586.1"
                     /translation="MKIPKLRSFVCAVPALFAVTVYGQEAATDDEVVRLSAFEVSAKA
                     SRGYVTTSSMSASRIAVPITELPSSVVVINEKMIEDTVAVDLRDTLSLVSGILQSAPP
                     QGSNEISMRGYTLSGAQRDGVADYLLSSGSEAGGGFDYALTERIEVVKGPSGILYGAH
                     NPGGIINLISKRPLSTPKTRITLMGGSYNSYRAELDTSNFFDA"
BASE COUNT          349 a          501 c          544 g          337 t
ORIGIN      
        1 acgcgatcgc gccaagcggt gtcgcattcc tcgccatcct gatcgaagaa acggccgcac
       61 acttcgccga cggcgccggc ggcgcgcagc tcggcgacgt cggccggttc gagaacgccg
      121 cgctcgacaa acacggaatt gtccagggtg ccgagaccga cgagggcgaa tgtggcgcgg
      181 ctgagttgtt cgtggacttc gttgacctgc gcgagcttca acaaggcgtc gcgggtgcgc
      241 ttgtcgggaa tgtaagcagg ggcgttgagc gagaggaaaa cgccgccgag ccgctgcgcc
      301 atcacgcgcc cgacttcctg tgcgtcaaac gcactcacgt tggaatcgac gctgcccatg
      361 gcctggatga ccgtgagcgc tttgttgcgc acttcggaca gatgatgaac gagctcgtga
      421 atcgtgcgtc cgccggcgat cgcgacgatg tcgcgcggct ggatgagcgc ctcgaccgcg
      481 gtagaggcga aatggccgac ggaacgacga aggtcgacgc tcggcagctc ttcgatcgcc
      541 ttgatgacaa tcacggtggc gaggccgagc tgtgcgcgga gctgcgcctc cagctcggct
      601 tgacgaggtt cgtagtcagc aacggtgatg cgcacgatcc cgcgctcccg agcgagagcg
      661 agcagtcggg agaccttagc ttgagagacc ttcacaaact tggccacatc ggcctgcccc
      721 aagccatcga tgtagtagag ccgggccgct agccgcaggc gatcatcgga atatttattc
      781 tgcatacgaa ttagagaact gatacaggcc gtggtttttt attgtcaagg agtgattgta
      841 acggcgcatc tgcgcttggg ggtaaaatgg aaaacagccc acggcgccga gtaataggga
      901 gggttatttt ccgagcgaaa ctttatcttg gcaccgcgtc gagccggctg aagtccaatc
      961 ataaagggcg gatgccaacc aggtgacgaa gcggaaacag acttgacacg ccaaacgggg
     1021 cagggattga tttcgtatgt taattctgaa taattattca ggattatgcc cccgggttcc
     1081 gcagtctccc tcggcaacct cacctttccc gtttccccga catgaaaata cccaagctcc
     1141 gttcgtttgt gtgcgccgtt ccggcgctgt ttgcggtcac cgtttacggc caggaggccg
     1201 ctaccgatga cgaagttgtg cggttgtctg cctttgaggt ctcggccaag gcaagccgcg
     1261 gctacgtgac tacgtcctca atgagtgcct cgcgcatcgc ggtgccgatc accgagctgc
     1321 cgtcgtcggt ggtggtgatc aacgagaaga tgatcgaaga caccgtggcg gtggatcttc
     1381 gcgacacgct cagcctcgtg agcggtattc tccagagcgc gcctccgcag gggagcaacg
     1441 aaatctcgat gcgcggctac acgctttccg gcgcgcagcg cgacggtgtg gccgattacc
     1501 ttttgtcctc tggcagcgaa gcgggcggcg gtttcgacta cgcgctgacc gagcgcatcg
     1561 aggtggtgaa gggcccgtcg ggcattctct acggagcgca caatccagga ggcatcatca
     1621 atctgatcag caagcgcccg ctcagcacac cgaagacgcg gatcacgctg atgggcggct
     1681 cgtataactc gtatcgcgcc gagctcgata cctcgaattt cttcgatgct a
//
LOCUS       DASPLT010000581         2562 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_108548, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000581 DASPLT010000000
VERSION     DASPLT010000581.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 2562)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2562)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2562
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_108548"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..428
                     /locus_tag="VEA63_06030"
     CDS             <1..428
                     /locus_tag="VEA63_06030"
                     /inference="COORDINATES: protein motif:HMM:NF013155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD83587.1"
                     /translation="QHSRRTLVEVNLISNRLLEYTPDLAHHPFPEFLRTGVPVCLNTD
                     DRGMWDSNLTDEYYTAVTQFNLSWDEIVQLGRNSLAYAFVQPDVKQQLLARYEAALAA
                     FEKKYGTDAATSEAALAQLATVKPTAYGYARRTWQLTFP"
     gene            complement(544..1395)
                     /locus_tag="VEA63_06035"
     CDS             complement(544..1395)
                     /locus_tag="VEA63_06035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007362197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HYD83588.1"
                     /translation="MSRLLEPFQYPFMVEAMVVGVAIGAVCAVLSCYLVLKGWSLMGD
                     AISHAVLPGIVIAYTIGVPLAIGAVASGLTCAVLTGYVNANSRVKEDTVMGVVFTGMF
                     AFGLVLFTKIESDLHLDHILFGNILGLAAGDLRDTLIVGGVTLAVVLALRRDLLLFCF
                     DAAHARTIGLRTTVLYYLLLSLLAVTIVVSLKAVGIILVIAMLVTPGCIGYLMTDRFN
                     AMMTIAAASAVFSSFVGVYLSFFINASTGACIVLLQAAIFVIALIWAPKHGMLAARRH
                     RAAARSA"
     gene            complement(1392..2315)
                     /locus_tag="VEA63_06040"
     CDS             complement(1392..2315)
                     /locus_tag="VEA63_06040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007362198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HYD83589.1"
                     /translation="MIETLLIPLQYDYMVKAILVSGVIGGVCAFLSCFITLRGWSLMG
                     DALSHSVVPGVAIAYYIGWPFAAGALVTGLLAAAGMGFVKTKTRLREDAVIGVVFTAF
                     FALGLFLISLNPSGVDLRSIVFGNILGISDSDIVQMLIISAVTLLALGAKWRDLLLYC
                     FDANQARALGLNTTVLHGLLLTLLSATAVAALQTVGAVLVVAMLVTPGATAYLLTDRF
                     GRMIGLAVAMGAGTSLVGAYASYFFDGSTGGCIVTLQSALFVIALVFAPKHGIRAARA
                     KRRAEAREYDCGSGDESGSCAHTQNHTEGRP"
BASE COUNT          579 a         1010 c          579 g          394 t
ORIGIN      
        1 ttcagcacag ccggcgcacg ctcgtcgagg tgaacctcat ctcgaatcga cttcttgaat
       61 acacgcccga tctcgcgcac cacccgttcc ctgagtttct gcgcaccggc gttcctgtct
      121 gcctcaacac cgacgaccgc ggcatgtggg acagcaacct gaccgacgaa tactacaccg
      181 ccgtcacgca gtttaatctt tcctgggacg agatcgttca actcggccgc aactccctcg
      241 cctacgcctt cgttcaaccc gacgtgaaac aacaactcct cgcccgctac gaagccgccc
      301 tcgccgcctt cgagaaaaaa tacggcaccg acgccgccac ctccgaagcc gccctcgccc
      361 aactcgccac cgtgaaaccc accgcctacg gctacgcccg gcgcacgtgg cagctcacct
      421 ttccctgacg cgccaccgtc cgttctgcag gtagggtcgc ctctccgagg cgtccgcgca
      481 ggcgattcct cgctcgccga catcgcagtc tccgcggttt gcgggagcgg cgtcctctgt
      541 gcttcacgcc gagcgcgccg ccgctcgatg ccgccgcgcc gccagcatcc cgtgcttcgg
      601 cgcccagatc agcgcgatca cgaaaatcgc ggcctgcagc aacacgatgc atgcgcccgt
      661 cgacgcgttg atgaaaaagc tcagatacac gccgacaaaa ctcgagaaca ccgcactcgc
      721 cgccgcaatc gtcatcatcg cgttgaagcg gtctgtcatc aaataaccga tacaccccgg
      781 cgtcaccagc atcgcgatca cgagaatgat gcccaccgcc ttcaacgaca ccacgatcgt
      841 caccgccagc agcgacaaca gcaggtaata gagcaccgtc gtccgcagcc cgatcgtgcg
      901 cgcatgcgcc gcatcgaagc agaacagcag caggtcccgc cgcagcgcga gcaccaccgc
      961 gagcgtcact ccgccgacga tcagcgtgtc gcgcaaatcc cccgccgcca atccgaggat
     1021 gttgccgaat aaaatatgat ccaggtgcag gtcgctctcg attttcgtga agagcaccag
     1081 gccaaacgcg aacatcccgg tgaacaccac gcccatcacc gtatcctcct tcacccgact
     1141 gttcgcattc acgtagcccg tcagcaccgc gcacgtcagc cccgacgcca ccgccccgat
     1201 cgccagcggc acgccgatcg tatacgcgat cacgatcccc ggcagcaccg catgcgaaat
     1261 cgcgtcgccc atgagcgacc agcctttcaa caccaggtaa cacgacaaca ccgcgcacac
     1321 tgcgccgatc gccacgccga cgaccatcgc ctcgaccatg aacggatact gaaacggctc
     1381 gagcaaccga ctcatggccg cccctccgtg tggttctgcg tgtgagcgca ggaaccgctt
     1441 tcatccccgc tgccgcagtc atattccctc gcctccgccc gccgcttcgc ccgcgccgcg
     1501 cgaatcccat gcttcggcgc aaacaccagc gcgatcacga acaacgcgct ctgcagcgtc
     1561 acgatgcaac cgccggtcga cccatcgaaa aaataactcg catacgcacc gaccagcgac
     1621 gtccccgccc ccatcgccac cgccagcccg atcatccgcc caaaccgatc cgtcaacaaa
     1681 tacgccgtcg cacccggcgt caccagcatc gccacgacga gcaccgcgcc gaccgtctgc
     1741 aacgccgcga ccgccgtcgc cgacagcaac gtcagcaaga gtccgtgcaa cacggtcgtg
     1801 ttcagcccca gcgcgcgcgc ctgattggca tcgaagcaat acagcaacaa atcccgccac
     1861 ttcgctccca gcgccaacag cgtcaccgcc gaaatgatca acatctgaac gatgtccgaa
     1921 tccgaaatcc ccaaaatatt tccaaacacg atcgatcgca gatcgacccc gctcggattc
     1981 agcgaaatca aaaacaaccc cagcgcgaag aacgccgtga acaccacgcc aatcaccgca
     2041 tcctctcgca gccgcgtttt cgtcttcaca aatcccattc ccgccgccgc caacaaaccc
     2101 gtcactaacg ccccggcggc gaacggccac ccaatataat aagcgatcgc cactcccggc
     2161 accaccgagt gcgacagcgc gtcgcccatc aacgaccagc cgcgcagcgt gatgaagcac
     2221 gacaaaaacg cgcacacgcc tccgatgacg ccactcacca gaatggcctt caccatatag
     2281 tcatactgca acggaatcag cagcgtctca atcatgacag ctctcttcaa accgtctcgg
     2341 gcgaggcctc tgaaatctta aatccaaaat ctgagatcca aaatccaaaa ctcctcccgc
     2401 aggcgcactc agcgccctct caagcttccc gatccccgat gccaaattcc cgatttccga
     2461 tttcaagatt tccgctctca tcatcacacg tcctcctcgc cctccgcccg cttcttcacc
     2521 acgtcctcgc gcccgcgccg ctcgctatac tcgacgtgcc cg
//
LOCUS       DASPLT010000582         1729 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_192047, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000582 DASPLT010000000
VERSION     DASPLT010000582.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1729)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1729)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1729
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_192047"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..620)
                     /locus_tag="VEA63_06045"
     CDS             complement(<1..620)
                     /locus_tag="VEA63_06045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD83590.1"
                     /translation="MNPRPTPLVLFAALTLALVSVPTFAHASPQHERIAAAYVIALGR
                     TPSSSEIDRWSKEEKISVPKLVAELEQNLSQDRALQGTVRRKAFEDAFGRTPSDTEVA
                     AGAESGATSYTTLLKRHLEQLAKQPDDYAQVLDRAYRLVVRRGVYPEEIAYWKSRDTL
                     SYALLVGCIEDWARRNQPGLMVTAGTPTVSINSGYLTTVRLSRPVA"
     gene            complement(743..1267)
                     /locus_tag="VEA63_06050"
     CDS             complement(743..1267)
                     /locus_tag="VEA63_06050"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HYD83591.1"
                     /translation="MSSPSRLADSRGCAIDKPVMELFIVRHAHAVDAEENPERPLSSR
                     GREQVRQLTAFLEKSGSLNCGEVWHSPLARSRETAELLTAGLALNARLVEVDGLEGEA
                     DPTIIVGRLQSRREPLAIVGHEPHLSALASQLVAGTAEPPRFVLKKCAALALERGDDI
                     WRVRWHVSPEILVG"
     gene            1460..>1729
                     /locus_tag="VEA63_06055"
     CDS             1460..>1729
                     /locus_tag="VEA63_06055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD83592.1"
                     /translation="MKTLIKLLALAAALSLPTINAVAGYHSTTASASNGSNDQSGFNI
                     GIPNGGAAGYVLVISGYGYVSASVSGSGLSIGGSRDTDGSEGNGGA"
BASE COUNT          325 a          495 c          580 g          329 t
ORIGIN      
        1 tccgccacgg gacgcgatag ccggacggtg gtgagataac cgctgttgat ggagacggtg
       61 ggcgtgcccg cggtgaccat caagccgggc tgattgcgtc gcgcccagtc ctcgatgcag
      121 ccaaccagca gcgcgtagga aagcgtgtcg cggcttttcc agtaggcgat ttcctcggga
      181 taaacgcccc ggcgaacgac gaggcgataa gcgcgatcga gaacctgcgc gtagtcgtcg
      241 ggctgcttgg cgagttgctc gagatgacgt ttgagcagcg tggtgtagct tgtcgcgcca
      301 ctctcggcac cagcggcgac ttcggtgtcg gagggcgtgc ggccgaaagc atcttcgaag
      361 gctttgcggc gaacggttcc ttgcagcgca cggtcctggc tcaagttctg ttcgagctcg
      421 gcgacgagtt ttgggacgga aattttttct tctttgctcc aacggtcgat ctcgctggac
      481 gatggagtgc ggccgagagc gatcacataa gcggcggcga tgcgttcgtg ttgtggcgac
      541 gcgtgggcaa acgtcggcac ggaaacgaga gccagggtga gcgcggcgaa cagaaccagc
      601 ggggtagggc ggggattcat gagtggagca agacgacagc gcgagtcatg agatgcgtca
      661 aatctgtggc gacacgcgcg cgtgcctgag cgagggtgtg caggctttcg ccttcatgct
      721 gggtttaact gcaagcttcg gctcagccca cgaggatttc cggcgacacg tgccaacgca
      781 cgcgccagat gtcgtcgcca cgctcgagcg cgagcgccgc acatttcttc aacacgaagc
      841 gcggcggttc ggccgtgcca gcgacgagtt gcgaagccag tgcgctgagg tggggctcgt
      901 gaccgacgat cgcgagcggt tcgcgacggc tttggagccg gccaacgatt atcgtcggat
      961 cggcttcacc ctcgaggccg tcgacctcga cgagccgggc gttcaacgcg aggcctgcgg
     1021 tcagcagttc ggccgtctcg cgagaacgtg cgagggggct gtgccaaacc tcgccgcagt
     1081 tcagcgagcc gcttttttct aaaaaggcgg tgagctggcg gacctgttca cggccgcgtg
     1141 agctgagcgg tcgctcagga ttttcttccg cgtccacggc gtgagcgtga cgaacgatga
     1201 agagctccat gacgggttta tcgatggcgc agccgcgcga atccgcaagg cggctgggcg
     1261 atgacacggc cggagcgtgg gaaaggctct tatgtgagag cggcaatgat tttgcgtcta
     1321 actcgaatta ttgcaccctc atggcgttct tggcgtgcgc ggttttaccc tgcacccgac
     1381 gaattttcgc cgtgtcgcgc cgtttccaac gatcgcagtc gcggcggaat aaccgaaaaa
     1441 cccccacaat cgaataccca tgaaaaccct gatcaaacta ctcgccctcg ccgcagcttt
     1501 atcgctcccg acgatcaacg ctgtcgccgg ctaccactcg acgactgcca gcgcgtcgaa
     1561 cggcagcaat gaccaatccg gattcaacat cgggatcccc aatgggggag ctgccggata
     1621 tgtgctggtg atttccggtt acggttacgt tagcgcgagc gtaagcggct ccggcttgag
     1681 catcggtggg agccgcgaca cggacggttc cgagggcaat ggtggtgcc
//
LOCUS       DASPLT010000583         1174 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_252880, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000583 DASPLT010000000
VERSION     DASPLT010000583.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1174)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1174)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1174
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_252880"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(264..929)
                     /locus_tag="VEA63_06060"
     CDS             complement(264..929)
                     /locus_tag="VEA63_06060"
                     /inference="COORDINATES: protein motif:HMM:NF012804.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HYD83593.1"
                     /translation="MSSALHESLKAEMIRTCRAMERIGHFIGTWGNISVRVSEGFIVT
                     PSRVNYDAITPADFVTVSLEGGIIAGHRLPSSETGVHRLVLKKKPDVGAIIHSHPPYA
                     SAVACLQRSIPVFVEDIAQIIGGEVKCTRYVPAGQHEKIAAEVANTIGDENAVLLANH
                     GALVCGRTLDEAFVACQILEKAAQMLLLTEGAGGPLPIPEEHVRSERHRFLHKYGTAN
                     DRG"
     gene            complement(965..>1174)
                     /locus_tag="VEA63_06065"
     CDS             complement(965..>1174)
                     /locus_tag="VEA63_06065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD83594.1"
                     /translation="GAKGAAINVGVAGGFYHNARHGVKESVRVSRTYSPDRTATKLYA
                     QLYPIYRETAQRQMELWDEKARIFA"
BASE COUNT          228 a          369 c          347 g          230 t
ORIGIN      
        1 cgcgatgttc ctccgggcca catcgagatc gagaatcagc gccggcgtga cgagctcccg
       61 gcggtgacgt ccgatcgcgg gcgcgtaagc gcggcgaatg tgatcacgaa gttgatcgat
      121 catagttcga gaacgcgctc ggaatttccc ggccgattca tgaccgcaaa aaagcgcgac
      181 gagacgcaaa aaccagagtg gcttccgtgc tcccttcggt gcgttttgtg ccctttcgtg
      241 gccatcgttc cggttgaaca ggtctaacct ctgtcgtttg ccgtcccgta tttatggagg
      301 aagcgatgcc gctcggagcg gacgtgctcc tcgggtatcg gaagcggacc gccagccccc
      361 tccgtgagca gcagcatctg cgcggccttc tcgagaatct ggcaggcgac gaacgcctca
      421 tcgagggtgc ggccgcagac gagcgcgccg tgattcgcga gcaacacggc gttctcgtcg
      481 ccgatcgtgt tcgccacctc tgccgcgatt ttctcgtgct gacccgcggg cacatagcgc
      541 gtgcacttca cctcgccgcc gatgatctgc gcgatgtctt caacgaagac aggaatcgag
      601 cgctgcaagc acgcgaccgc gctcgcgtaa ggcggatgag agtgaatgat cgcaccgaca
      661 tccggtttct ttttcaaaac cagccggtgc actcccgttt ccgatgatgg caaacgatgg
      721 ccagcaatga tgccaccctc cagactcacg gtcacgaaat ccgccggcgt gatcgcgtcg
      781 tagttcaccc gactgggcgt gacgatgaaa ccctcggata cccgcacgct gatgtttccc
      841 cacgtcccga tgaaatgacc gatgcgctcc atcgcacggc aggtgcggat catctcggcc
      901 ttgagagatt cgtgaagagc ggaggacatc gaaggcgaaa tcaggatggc ggcacactac
      961 tggctcaggc gaagatccgt gctttctcat cccaaagctc catctgccgc tgcgcggttt
     1021 cccggtagat cggataaagc tgcgcgtaga gcttcgtcgc cgttcgatcg ggagaatacg
     1081 tgcgactcac gcgcaccgat tccttcacgc catggcgcgc gttgtggtag aacccgcctg
     1141 cgacaccgac gttgattgcc gcgccctttg cgcc
//
LOCUS       DASPLT010000584         1557 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_7587, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000584 DASPLT010000000
VERSION     DASPLT010000584.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1557)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1557)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1557
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7587"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..576
                     /locus_tag="VEA63_06070"
     CDS             <1..576
                     /locus_tag="VEA63_06070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD83595.1"
                     /translation="IATHALDDDVRGVMFGALVGAASLLLAVMLGVLAIVTTLDGRPI
                     MVRWRKQPRSYERLIDALLGPVFTTLVMAALSIVCVAIPAEGANGDVVAFVVALTLAL
                     GVATFFQAAYVGYILSRILLAGENAGTTRSAQTAEPPTTIAPALDPPAPESTVVAAPE
                     PPPVVAPKPSKGRQSKKRGGQQARVAIASGT"
     gene            853..>1557
                     /locus_tag="VEA63_06075"
     CDS             853..>1557
                     /locus_tag="VEA63_06075"
                     /inference="COORDINATES: protein motif:HMM:NF022021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P63C domain-containing protein"
                     /protein_id="HYD83596.1"
                     /translation="MGKLPLCGHEIPCANLEDGRRVISITAFSAALGRSSPGAQTYRR
                     RAEQGVDHLPIFLSSKRLSPFIPSDFSVSTIRYTPVGNGAMAEGIDAQAVPMICGIWV
                     KAWAADALVAHQIPTAQKAAAIATALAHVGIAALVDEATGFQYDRDREALSVILEQYL
                     GKELAAWTKTFPDDFYRHLSKLRGIRYDDQKRPQYFGMLTNDIIYSRLAPGVRKELCT
                     RNPKLDSGRRRFKHHQF"
BASE COUNT          292 a          492 c          492 g          281 t
ORIGIN      
        1 atcgcgacac acgcactcga cgacgacgta aggggcgtga tgttcggagc cctcgtcgga
       61 gcagcatcgt tgctgctggc cgtgatgctc ggcgtcctcg cgatcgtcac gacccttgat
      121 gggcgtccaa tcatggtccg atggcgcaag caacctcgca gctacgagcg cctaatcgac
      181 gcactcctcg ggcccgtgtt caccacgctt gtgatggcgg cactcagcat tgtctgcgtc
      241 gcaattcccg ctgaaggtgc gaacggtgac gtcgtcgcgt ttgtcgttgc gctgaccctc
      301 gcgctgggtg tcgcgacgtt cttccaggcc gcgtacgtcg ggtacatctt gagccgcatc
      361 ctccttgcag gggagaacgc cggcacgacc cgctcagctc aaacagcaga gccgccaacc
      421 acgatcgctc cggctctcga tccacctgct ccggaatcga cggtcgttgc cgcgcccgag
      481 cctccgccgg tggttgcccc aaaaccatcg aagggacgcc agtcgaagaa gcgcggtggc
      541 cagcaggctc gagttgcaat cgcatccggc acgtagcccc cggagtcaaa ccgcgggcgc
      601 aagcagttgt gatcgcaccg cttttatata tgcctacgta agagtgtcag gcccgtgcgc
      661 tatgcgtagg gtgtgaaaag gcgatcggat gatgggaaga gcgtcgaggg cgagttcgcg
      721 gtcgacaaga ccgatgcgcg cgtgaccggc gcgatcggcg gtcggacgcg tgcggcgaag
      781 atgtcagcgg gcgcgcggaa gcgtgcgtca aaggacgccg taaccgttcg atggggtgtc
      841 cgcgcgacgc acatgggcaa gcttccgctt tgcggccacg agattccgtg cgcaaacctg
      901 gaggacggac gacgagtcat ctcgatcaca gccttctcag cagcccttgg ccgttcgtcg
      961 cctggtgcgc aaacgtatcg ccggcgcgcc gagcagggtg tcgaccatct gccgattttt
     1021 ctatcgtcga agcggctaag ccctttcatt ccaagcgact tctcggtatc gaccattcga
     1081 tacacgccgg tgggcaacgg cgcgatggcg gaaggaatcg acgcgcaggc cgtgccgatg
     1141 atctgcggca tttgggtgaa ggcgtgggcc gctgatgccc tcgtcgcaca ccagatccca
     1201 acggcgcaga aggcagccgc gatcgcgaca gctcttgcgc acgtcggcat cgcggcccta
     1261 gtcgacgaag cgacggggtt tcagtacgac cgcgatcgcg aggcgctgtc ggtcatcctc
     1321 gagcagtacc tcggaaaaga gctcgccgcg tggacgaaga cgttcccaga tgatttctat
     1381 cggcacctct cgaagcttcg cggcattcga tacgatgatc agaagcggcc gcagtacttc
     1441 ggcatgctga cgaacgacat catctactcg cgcctcgccc cgggcgtccg caaggagctg
     1501 tgcacgcgca acccgaagct cgattcaggt cggcgtcggt ttaaacacca ccagttc
//
LOCUS       DASPLT010000585         1219 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_89556, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000585 DASPLT010000000
VERSION     DASPLT010000585.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1219)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1219)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1219
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_89556"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1049)
                     /locus_tag="VEA63_06080"
     CDS             complement(<1..1049)
                     /locus_tag="VEA63_06080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HYD83597.1"
                     /translation="MRLLRLSLGCALAFSLLTACSRREAPAEAAIASRTLLLGNLSEP
                     AELDPHLATAYTDQNILIALFEGLTALDEKSSLPVPATAARWEVSPDGLTYTFHLRPD
                     ARWSNGDRVTAHDFAFSFQRILSPALAAEYSYMLWPIKNAEAFNTGRVTAFSDVGVAA
                     IDDATLRLTLERPTPYLLSLAAHPTWFPVHRATIEKFGRADQRSTAWTRVGHLVGNGA
                     FTLTEWSPNARLTVSENPLYWGSADNALERVTFFPTENPDVEERDFRAGQTHLTYSLP
                     IEKIARYRNDSPEQLRLDPFLQTFFLRFNTTRPPLDNPRVRRALALALDRDAITAHIL
                     RGSRLPAAHLTPPDCA"
BASE COUNT          230 a          283 c          502 g          204 t
ORIGIN      
        1 gcacaatcgg gcggggtgag gtgcgcagcg ggaagacgcg agccccggag gatgtgtgcg
       61 gtgatcgcat cgcgatcgag ggcgagggcg agggcgcggc ggacgcgggg attgtcgagc
      121 ggcgggcgcg tggtgttgaa acggaggaag aacgtttgaa gaaacggatc gagccggagt
      181 tgctcgggcg agtcgttgcg atagcgggcg attttctcga tgggaaggct gtaggtgaga
      241 tgggtctgtc cggcgcggaa atcgcgttcc tcgacgtcgg gattttcggt ggggaaaaaa
      301 gtcacgcgtt cgagggcatt gtcggcagag ccccagtaga gtggattttc ggatacggtg
      361 aggcgggcgt tgggcgacca ttcagtgagc gtgaaggcgc cattgccgac gaggtggccg
      421 acgcgggtcc acgcggtgct gcgctgatcg gcgcggccaa acttttcgat ggtggcgcgg
      481 tggacgggaa accaggtcgg gtgcgcggcg agcgagagca gataaggcgt ggggcgctcg
      541 agcgtgaggc ggagcgtggc gtcgtcgatc gcggcgacgc ccacgtcgga gaacgcggtg
      601 acgcggccgg tgttgaacgc ttcggcgttt ttgatcggcc aaagcatgta ggaatattcc
      661 gcggcgagcg cgggagagag gatgcgttga aaggagaacg cgaaatcgtg cgcggtgaca
      721 cgatcgccgt tggaccaacg ggcgtcgggg cgcaggtgaa atgtgtaggt gaggccgtcg
      781 ggggagacct cccagcgtgc ggcggtggcg gggacgggga gcgaggattt ttcgtcgagc
      841 gcggtgaggc cttcgaataa cgcgatgagg atgttctgat cggtgtaggc ggtggcgaga
      901 tgcggatcga gctccgccgg ttcggagaga ttgccgagca atagcgtgcg cgatgcgatc
      961 gcggcttcgg cgggagcttc gcggcgcgag cacgcggtga ggagcgagaa ggcgagagcg
     1021 cagcccaagg aaaggcgcag gagacgcatg agggaaatga cgaacgcgat cggatttgct
     1081 gcaagttcgg cgcgggcccc gatatttcat tgcaagccga tgtctgcggc ggtttcaaag
     1141 gagccgcttc acctatgcaa tggtattacg ccgtcgacgg gcagcgccag ggcccggtct
     1201 ctgctgagga attcgagca
//
LOCUS       DASPLT010000586         1107 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_108606, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000586 DASPLT010000000
VERSION     DASPLT010000586.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1107)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1107)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1107
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_108606"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..333)
                     /locus_tag="VEA63_06085"
     CDS             complement(<1..333)
                     /locus_tag="VEA63_06085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HYD83598.1"
                     /translation="MPKYTLNVNGQSRSVDVAADTPLLWVLRDSLDLVGTKYGCGIGQ
                     CGACTVHLDGVPTRSCLTPVSTATSLQITTIEGLAADAEHLHPVQQAWCDLDVAQCGY
                     CQAGQIMTA"
     gene            complement(513..>1107)
                     /locus_tag="VEA63_06090"
     CDS             complement(513..>1107)
                     /locus_tag="VEA63_06090"
                     /inference="COORDINATES: protein motif:HMM:NF028108.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF5069 domain-containing protein"
                     /protein_id="HYD83599.1"
                     /translation="RDRHPRRVAFLASLHESLRAMPHYEFTSQFRRIYDHAAALYVKG
                     QRSAETFFDAGERAFLAANGITPQHLYDYAEDQANYGEPGYDNALAIELVRRDYFLNV
                     QDGRASSVVLDETSLPAKTDAVRGIEWLPRIIPKAKAKLRGELPPSLMYCCGGDRKFL
                     KAHDILPSEFLALVWRHENDDTAIVEWVVRRSAAKQK"
BASE COUNT          208 a          295 c          397 g          207 t
ORIGIN      
        1 ggcggtcatg atctggccgg cctggcagta accgcattgg gcaacgtcga gatcgcacca
       61 agcttgttga acagggtgca agtgctcggc gtccgcagcg aggccttcga tcgtggtgat
      121 ctgcaggctg gtggcggtcg acaccggcgt gaggcaggag cgggtcggaa cgccgtcgag
      181 atggacggtg caggcgccgc actggccgat gccgcaaccg tatttcgttc cgacaaggtc
      241 gagggaatcg cggagcaccc acaggagggg cgtgtcggcg gcgacatcca ccgagcggga
      301 ctggccgtta acgttcagag tgtatttggg catgagaggc ggggttgtcg cttcagcaaa
      361 gtgatcggag aggccgcaaa aaagcttttt ggcggtcgca cgggtgacgc ggtcacggcg
      421 aaaaagttgc ggcgccgcga agcgtggttt cccctaaact cagccaataa aaaacggcgg
      481 tcgatgaccg ccgttgcgag cgcgcgctct gtttattttt gtttcgcggc gctgcggcgc
      541 acgacccatt cgacgatcgc ggtgtcgtcg ttttcgtgac gccacacgag cgcgagaaac
      601 tcggacggca ggatgtcgtg ggctttcagg aatttccgat cgccgccgca gcaatacatc
      661 agcgacggcg ggagctcgcc gcggagcttg gctttcgcct ttggaatgat gcgcggcagc
      721 cactcgatgc cgcgaaccgc gtcggttttt gcgggcaacg aggtttcatc gaggacgacg
      781 gacgacgcgc ggccgtcctg gacgttgaga aaataatcgc ggcgaacgag ttcgatcgcg
      841 agggcgttgt cgtagcccgg ttcgccgtaa ttcgcctgat cttcggcgta atcgtaaaga
      901 tgctgcggcg tgatgccgtt ggcggcgaga aaagcgcgtt cgccggcgtc gaaaaacgtc
      961 tcggcgctgc gctggccttt gacgtaaagc gcggcggcgt gatcgtagat gcggcggaac
     1021 tgggaggtga actcgtagtg tggcatggcg cgcaacgatt catgcaggct tgcgagaaag
     1081 gcaactcggc gcgggtggcg atcgcgg
//
LOCUS       DASPLT010000587         1153 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_272982, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000587 DASPLT010000000
VERSION     DASPLT010000587.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1153)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1153)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1153
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_272982"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..236
                     /locus_tag="VEA63_06095"
     CDS             <1..236
                     /locus_tag="VEA63_06095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HYD83600.1"
                     /translation="WPRVRIGEIVFRAGGPCARCIVTTTDQFTGARGKEPLRTLATYR
                     RDTSDPSAVNFGQNLIHETKSGTLRVGDLVSIV"
     gene            complement(270..713)
                     /locus_tag="VEA63_06100"
     CDS             complement(270..713)
                     /locus_tag="VEA63_06100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009510874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HYD83601.1"
                     /translation="MIIGGYAAVAHGSAQVTRDIDVCAVLTSENVETLRRALAEWKPK
                     HRMTSQRLSFLDVPPPGTPVNSLYLQTDVGVVDVLSSVLGVGDFERLKSRAEKLEVDG
                     RIHWLIALEDLIAAKEAVGREKDLLVVKELRAIAAKRRALDGAGG"
     gene            complement(730..924)
                     /locus_tag="VEA63_06105"
     CDS             complement(730..924)
                     /locus_tag="VEA63_06105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007362149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD83602.1"
                     /translation="MQTPDPLDTAAKAGIDLDLLDTNLALPVKERWRQHDGALAFILK
                     LEEAKRKRDAGLQPVTREAR"
     gene            complement(968..>1153)
                     /locus_tag="VEA63_06110"
     CDS             complement(968..>1153)
                     /locus_tag="VEA63_06110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor-like protein"
                     /protein_id="HYD83603.1"
                     /translation="RELGEKLEARERERTAAVQQAREAERRRTRPKSRAQKVRMIEAK
                     KRRAKHKAARGRVGREE"
BASE COUNT          200 a          438 c          292 g          223 t
ORIGIN      
        1 actggccgcg cgtccgcatc ggtgaaattg tttttcgcgc cggcggcccc tgtgcgcgct
       61 gcatcgtgac gaccaccgac caattcaccg gcgcccgcgg caaggaacct cttcgaaccc
      121 tcgccaccta tcgccgcgat accagcgacc ccagcgccgt caacttcggc caaaatctca
      181 ttcatgaaac caagtccggc accctccgcg tcggcgacct cgtctcgatc gtataaaaag
      241 gtaggggcgg ctgtcccagc cgaccggagt cagcctcccg cgccgtccaa cgcccgccgc
      301 ttcgcagcaa tcgcccgcaa ttccttcacc acgagcaaat ccttctcccg ccccacggcc
      361 tccttggccg cgattaggtc ctccagcgcg ataagccagt gaatacgccc gtcgacttcc
      421 agcttctccg cccggctttt caagcgctcg aaatctccca cacccaaaac cgacgacagc
      481 acgtcgacga cgccgacatc cgtctgcaga taaagactat tcacgggcgt gcccggcggc
      541 ggcacgtcca aaaaggacaa ccgctgcgat gtcatgcgat gcttcggctt ccactcggcc
      601 aacgcccgcc ggagcgtttc cacattctca ctcgtcaaca cggcgcatac atcgatgtcg
      661 cgtgtgactt gagcagatcc gtgcgccacc gcggcatagc cgccgatgat cacgaactcg
      721 aaccccgagt cagcgagctt cgcgagtaac tggctgaagt ccggcgtcac gtttgcgttt
      781 ggcttcttcc aatttgagga tgaacgccag cgcaccgtcg tgctgccgcc agcgctcttt
      841 caccggcaaa gccaggttcg tatccagcaa atccaagtcg attcccgcct tggcggcagt
      901 gtcgagtgga tcaggcgttt gcatgccgaa atatcgccgt gcgccgcggc ttcggcaacc
      961 tcgatcttca ctcttctctg cccacgcgac cacgcgcggc cttgtgcttc gcccgccgtt
     1021 tcttcgcctc gatcatccgc actttttgcg cccggctttt cggccgcgtc cgccgtcgct
     1081 ccgcctcacg cgcctgctgc accgccgccg tccgctcccg ctcgcgcgcc tcgagctttt
     1141 cgcccagttc gcg
//
LOCUS       DASPLT010000588         5739 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_150306, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000588 DASPLT010000000
VERSION     DASPLT010000588.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 5739)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5739)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5739
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_150306"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(239..2725)
                     /locus_tag="VEA63_06115"
     CDS             complement(239..2725)
                     /locus_tag="VEA63_06115"
                     /inference="COORDINATES: protein motif:HMM:NF037510.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HYD83604.1"
                     /translation="MNEAETRAELIDPALRAAGWGVVEQSRVRREQITKGRLLGAGIR
                     GEPTTADYVLIYRGKKLGVIEAKKRDAALTEGLGQAKAYAGKLQTRFAFSTNGQGFYQ
                     VDMQTGAEGEIAAFPTPDALWAATFAEENAWRDRFAAVPLSDHGGKQAHYYQDIAIER
                     VLEKITAGDKRILLTLATGTGKTFIAFQLAWKLFHARWNLTGEPTRRPRILFLADRNI
                     LADQAYNSFSAFAEDARARISPEEVRKTGRVPKNANIFFTIFQTFMAGASPFSLAEKG
                     LGDEGRSTASTPESLHAPHPPAPSPPRDRVAIPLILDSPAAKRPVGGGGEDRAYFYDY
                     PPDFFDFIIIDECHRGGAQDESSWRDIMEYFAPAVQLGLTATPRRQGNADTYRYFGEP
                     AFTYSLKEGINDGFLTPFKVRQFATTMDDYTYVADDIVLEGDVEAGDTFTENDFNTRI
                     EIMEREQNRVNLFMREIDQNQKTLVFCANQAHAAAVRDLINQAKTSRDPNYCVRVTAK
                     DGALGDHYLRAFQDNEKTIPTILTTSHKLSTGVDARNVRNIVLMRPIKSMIEFKQIIG
                     RGTRLYDGKDYFTIYDFVKAHALFSDPEWDGEPAEPDEGPKRPTIIGEPIAPIYDPED
                     PAPRPQRIKIRLADGKERRIQHMAQTMFYSPDGTPIPAAAFIEKLFGDLPELFKDEAE
                     LRTLWGDPTSRKRLISGLAERGYGPEQLHEIGKLIEAENSDVFDVLAYIAFALAPLSR
                     AERVTQHRAHIMSAYDSKLQTFLDFVLDQYVRSGVEELDAEKLPSLLTLKYQDLGDAV
                     AALGGVPAIRGAFLGFQPRLYGKSGGAQAR"
     gene            complement(2722..3930)
                     /locus_tag="VEA63_06120"
     CDS             complement(2722..3930)
                     /locus_tag="VEA63_06120"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013579.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HYD83605.1"
                     /translation="MLTIGELCEVVNGGTPKTGIDEYWNGKHLWITPAEMGKRESPYV
                     FETERRITDAGLRNSSARLLPPNSVILSSRAPIGHLVINTEPMAFNQGCKGLVPGPSI
                     HHKYLFYYLSSIVDLLNALGTGATFKELSGGKLKEVIVPVPPLAEQKRIVAILDEAFE
                     GIRIATANAEKNLANARDLFDAELRCLFEESAQSYPMLALENLTERITKGSSPKWQGI
                     SYVTDGGILFVTSENVGQNRMLLDEPKFVDARFNEKDRKSILRRDDVLTNIVGASIGR
                     TAVFESDDVANINQAVCLIRCKTDALLPRFLSALLNSDYFRRQLFANTTDTARANLSL
                     AFFRSFEIPVPPIEVQQRIIEDVLSIQLECDALSMRYQEKCNGVSELKQSILSRAFSG
                     QLTATKGLAA"
     gene            complement(3942..5393)
                     /locus_tag="VEA63_06125"
     CDS             complement(3942..5393)
                     /locus_tag="VEA63_06125"
                     /inference="COORDINATES: protein motif:HMM:NF014442.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HYD83606.1"
                     /translation="MFEQAFRNIDDVLRREAGCTTELDYTEQSSWILFLRYLDELETK
                     KRIAAELKGEPYAPIIDAKHRWSAWAAPKKPDNTFDHDNALTGDDLIAYVDNDLFPYL
                     RGFKQRAASADTIEYKIGEVFSEIKSKFTSGYNLRDVLEHTDELKFGSQAEQHELSLL
                     YEAKIKKMGNAGRNGGEYYTPRPLIRAMIKVLQPKIGERIYDGAVGSAGFLCEAYQHM
                     LPLAETTKQRETLQKRTFYGREKKSLAYVIALMNMILHGIETPNILHVNTLTENLADI
                     QEKDRYDIILANPPFGGSERKEVQQNFPIKTGETAFLFLQHFIKSLKAGGRAAIVIKN
                     TFLSNTDNAAIALRKELIQSCNLHTVLDCPGGVFQGAGVKTVVLFFEKGAPTRNVWYY
                     QLDPGRNLGKTKPLGDDDMADFVALQASFADSAKSWSVDAATLDELTCDLSVKNPNKA
                     EEAPLRDPKDIIDEMLALDAESAEILRGIRELV"
BASE COUNT          984 a         1566 c         1905 g         1284 t
ORIGIN      
        1 gacacctccc ccgtgaacgg gggaggaaag gtgactttgc ctttgcggtc ctttcctcat
       61 cgaagcgaca tcagccgcgc cggccgccgc ctgaccttcc aaagcttcgg tgcacttcga
      121 aatcctctcg tgtcaggcag agagagtatc gcacgggttt gggagggggc ggataagttt
      181 ttgtgctttt gctttgcgga caaagactta gggggaggga agtgggggat agattggctc
      241 agcgcgcctg tgcgccgccc gattttccat agagtcgcgg ctggaagccg aggaaggcgc
      301 cgcggatcgc aggaacgccg ccgagcgcgg cgacggcgtc gcccaagtcc tgatatttca
      361 gcgtcagcag gctcggcagt ttttcggcgt ccagttcttc gacgcctgag cgcacgtatt
      421 gatcgagcac gaaatccaag aacgtctgca gcttggaatc ataggcgctc atgatgtgtg
      481 cgcggtgttg cgtcacgcgt tcggcgcgag agagcggcgc gagcgcgaag gcgatgtaag
      541 cgagcacgtc gaagacgtcg ctgttttcgg cttcgatgag cttgccgatc tcgtgcagct
      601 gctcagggcc atagccgcgt tcggccagtc cggagatgag ccgtttgcgg ctcgtgggat
      661 cgccccaaag cgtgcgcagc tcggcttcgt ctttgaagag ttcaggcaga tcgccgaaca
      721 gcttttcgat aaacgcagcg gcggggatcg gcgtgccgtc cggcgaatag aacatggttt
      781 gcgccatgtg ctggatgcgg cgctctttgc cgtccgcgag cctgatcttg atacgttgcg
      841 gacgcggcgc ggggtcttcg ggatcgtaga ttggcgcgat cggctcgccg atgatcgtcg
      901 gccgctttgg tccttcgtcc ggctcagcgg gttcgccgtc ccattcggga tcgctgaaca
      961 gggcgtgcgc cttcacgaag tcgtagatcg tgaaatagtc cttgccatcg tagagccgcg
     1021 tgccgcggcc gatgatctgc ttgaactcga tcatcgactt gatcgggcgc atcagcacga
     1081 tgttgcggac gttgcgcgca tcgacgccgg tcgagagttt gtgcgatgtg gtgaggatgg
     1141 tcggaattgt tttctcgttg tcctgaaaag cgcgcaggta gtggtcgccc aaggcgccgt
     1201 cttttgcggt gacgcggacg caataattcg gatcgcggct cgtcttcgcc tggttgatga
     1261 gatcgcggac ggcggcggcg tgggcctgat tggcgcagaa gacgagcgtt ttctggttct
     1321 gatcgatctc gcgcatgaac aggttcacgc ggttctgctc gcgctccatg atctcgatgc
     1381 gggtgttgaa gtcgttctcc gtaaacgtat cgccggcttc gacatcgcct tcgagcacga
     1441 tgtcatcagc gacgtaggtg taatcgtcca tcgtcgtggc gaactggcgc accttgaacg
     1501 gcgtcaggaa gccgtcattg atgccttcct tgagcgagta ggtgaaagcc ggttcgccga
     1561 aatagcggta ggtgtcggcg ttgccttggc ggcgcggcgt ggcggtgagg ccaagctgca
     1621 cggcgggcgc gaaatattcc atgatgtcgc gccacgagct ttcgtcttga gcgccgccgc
     1681 ggtggcattc gtcgatgatg atgaagtcga agaagtcggg cgggtagtcg tagaaatatg
     1741 cgcgatcttc tccccctccc cccaccggcc gcttcgcggc cggcgaatct aagatcagcg
     1801 ggatcgcgac gcgatcccga ggcggggagg gggccggggg gtgaggggcg tgcaaactct
     1861 caggcgtcga ggcggttgaa cgcccctcat cccctaaccc cttctccgca agggagaagg
     1921 gggacgcgcc cgccatgaaa gtctgaaaaa tcgtgaagaa gatgttggcg ttcttcggca
     1981 cgcggcctgt cttgcgcact tcttcggggg agatgcgggc gcgggcgtct tcggcgaagg
     2041 cggagaatga attgtaggct tggtcggcga ggatgttgcg gtcggcgaga aagaggatgc
     2101 gtgggcggcg cgtcggttcg ccggtgagat tccagcgcgc gtggaagagt ttccaggcga
     2161 gttggaaggc gatgaaggtt ttgccggtgc cggtggcgag cgtgagcagg atgcgcttat
     2221 cgccggcggt gatcttttcg agcacgcgct cgatggcgat atcctgataa tagtgcgcct
     2281 gcttgccgcc gtgatcggag agcgggacgg cggcgaagcg gtcgcgccag gcgttttctt
     2341 cggcgaaggt cgcggcccag agcgcgtcgg gcgtggggaa ggcggcgatc tcgccttccg
     2401 cgccggtttg catatcgact tggtagaagc cttggccgtt ggtggagaag gcgaagcgcg
     2461 tttgcagctt gccggcgtag gctttggctt ggccgagccc ttcggtgagc gccgcatcgc
     2521 gcttcttggc ttcgatcacg ccgagcttct tgccgcgata gatcagcacg taatcggcgg
     2581 tggtcggctc gccgcggatg ccggcgccga gcaggcggcc tttggtgatc tgctcgcggc
     2641 gcacgcggct ttgctcgacc acgccccaac cggctgcgcg cagcgcggga tcgatcaact
     2701 cggcgcgtgt ctcggcctcg ttcacgcggc taggcctttg gttgcggtga gctggccaga
     2761 gaaggcgcgg gagaggatgg attgcttgag ttcgctcacg ccgttgcact tttcttggta
     2821 gcgcatgcta agcgcgtcgc attctagctg aatagacagc acgtcctcga tgatgcgttg
     2881 ctgaacttct ataggcggca ccgggatttc aaacgatctg aagaatgcaa ggctcagatt
     2941 ggcgcgagct gtgtcggttg tattggcgaa aagctgacgc cgaaaatagt cggaattcag
     3001 cagagcgcta agaaaacggg gcagtagggc atcggttttg catcgaataa ggcaaacagc
     3061 ctgattgatg ttggccacat catcggattc aaacactgcg gttcgaccga tagacgctcc
     3121 gacgatgtta gtgagaacgt cgtctcgccg aagtatggat ttccgatcct tctcattgaa
     3181 tcgcgcatct acgaatttcg gctcgtccag tagcattcga ttttgaccga cattctcgct
     3241 tgtgacgaag aggatgccgc catccgtcac gtaggatatg ccttgccatt tgggtgatga
     3301 ccctttggtt attcgctcgg tcaaattctc caacgcaagc atcggatacg attgagcgct
     3361 ctcttcgaat agacatctga gttcggcgtc aaacaggtcg cgggcgttgg cgaggttctt
     3421 ttcggcgttg gcggtcgcga tcctgatccc ctcgaacgcc tcgtccagaa tggcgacgat
     3481 gcgcttttgc tcagcgagcg gtggcacagg aacaatgact tccttgagct tgccgcccga
     3541 aagttctttg aaggttgccc ccgtgcccag cgcgttcagc aagtcaacga tgctgctgag
     3601 atagtagaag agatatttgt gatggatcga tggtccgggg accaatcctt tgcacccttg
     3661 gttgaatgcc atgggctccg tgttgatcac caaatgtcct atgggtgcac gcgacgacag
     3721 gattacggag ttcggaggaa gcaatctcgc ggaactgttt cgcaacccgg cgtcagtaat
     3781 ccgccgctct gtctcaaaaa catatgggct ctcacgtttc cccatttccg cgggcgtgat
     3841 ccagagatgc ttgccgttcc agtattcatc tatgccggtc ttaggtgtgc cgccgttgac
     3901 gacctcgcac agctcaccga tggtcagcat ccgccaagcc atcacaccag ctcccggatg
     3961 ccgcgtagaa tctccgcgct ctcggcgtcg agggcgagca tttcgtcgat gatgtctttc
     4021 ggatcgcgca gcggcgcttc ctcggctttg ttcgggttct tcaccgagag atcgcaggtc
     4081 agttcgtcga gtgtggcggc atcgacgctc cacgatttgg cgctgtcggc gaagctggct
     4141 tgcaacgcga cgaagtccgc catgtcgtcg tcgcccagcg gtttggtttt gccgagattg
     4201 cggccgggat cgagctggta gtaccagacg ttccgcgttg gtgcgccttt ctcgaagaac
     4261 agcaccaccg tcttcacgcc cgcgccctgg aacacgccgc cggggcaatc gagcacggtg
     4321 tgcagattgc agctttggat tagctctttg cgcagcgcga tggcggcgtt gtcggtgttc
     4381 gacaggaacg tgttcttaat gacgatggcg gcgcggccgc cggctttcag cgatttgatg
     4441 aagtgctgca ggaagaggaa ggcggtctcg ccggtcttga tcgggaagtt ctgctgcacc
     4501 tctttgcgct cgctgccgcc gaacggggga ttggcgagga tgatgtcgta gcgatccttt
     4561 tcctggatgt cggcgaggtt ttcggtgagc gtgttgacgt gcaggatgtt cggcgtctcg
     4621 atcccgtgca ggatcatgtt catcagcgcg atgacgtagg cgaggctctt tttctccctg
     4681 ccgtagaagg tgcgcttttg cagcgtctca cgctgcttcg tggtttccgc cagcggcagc
     4741 atgtgctggt aggcttcgca caggaagccg gctgagccga cagcgccgtc atagatgcgt
     4801 tcgccgattt tgggctgcag caccttgatc atggcgcgga tgagcgggcg gggcgtgtaa
     4861 tactcgccgc cattgcggcc ggcattgccc atcttcttga tcttggcttc gtagagcagc
     4921 gacagttcgt gttgttcggc ctggctgccg aacttcagtt cgtcggtgtg ctccaaaacg
     4981 tcgcgcagat tgtagccgct ggtgaacttg gatttgatct cgctgaacac ctcgccgatc
     5041 ttgtactcga tcgtatcggc gctggcggcg cgctgcttga agccgcgcag ataggggaag
     5101 aggtcgttgt cgacgtaggc gatgaggtca tcgccggtca gggcgttgtc atgatcgaag
     5161 gtgttgtcgg gctttttggg tgccgcccag gcggaccagc ggtgcttggc gtcgatgatc
     5221 ggggcgtagg gctcgccttt gagttcggcg gcgatccgct tcttggtctc gagctcgtcc
     5281 aggtagcgga ggaacagaat ccaggaggat tgctcggtgt agtcgagctc ggtcgtgcag
     5341 ccggcctcgc ggcgcagaac gtcgtcgata ttcctgaatg cttgctcgaa catggccccg
     5401 ccgcgatttg cccactggca aggtaacggg atttgccgcg ggcagggaag cttgggcggt
     5461 gcgctctatt ctggaccact ccccctcacc cggttcgagc aggctcgacc cgacctctcc
     5521 ccctcgcggg ggagaggtgg gcggtccata gtaccggctt cagccgctga aaacggtccg
     5581 gttctgcatt gacgggcacc cggcgcttcc cttatatcgc cgctccccgc ccgggcgagc
     5641 ttcgttcggg cgcgctatag gtttggaagc aagtccgatg ttcgcggtaa tcaagacggg
     5701 cggtaagcaa taccgggtgg ccaaggatga cgtgatcgt
//
LOCUS       DASPLT010000589         1155 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_130210, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000589 DASPLT010000000
VERSION     DASPLT010000589.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1155)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1155)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1155
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_130210"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..275
                     /locus_tag="VEA63_06130"
     CDS             <1..275
                     /locus_tag="VEA63_06130"
                     /inference="COORDINATES: protein motif:HMM:NF016296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LytTR family transcriptional regulator
                     DNA-binding domain-containing protein"
                     /protein_id="HYD83607.1"
                     /translation="AEDNYSTVTLASGERVLLRKSLSAWESILPASHFARVHRTRIVN
                     LAHVLRYERQGDGALLFIKGAAEPVPASRRTWSDVRTRLAELHPQL"
     gene            481..843
                     /locus_tag="VEA63_06135"
     CDS             481..843
                     /locus_tag="VEA63_06135"
                     /inference="COORDINATES: protein motif:HMM:NF016296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family transcriptional regulator
                     DNA-binding domain-containing protein"
                     /protein_id="HYD83608.1"
                     /translation="MPLRFDDTISLRTAGRAHFTSLRQIVLIAARDNYSEVTLSDGTR
                     VVLRKSLKAWQSLLPESHFLRVHRMLIVNLTSIVRYERDRDEHTRLFFAGRDTPVAAS
                     RRAWGLLRQRLADLHPIP"
BASE COUNT          178 a          453 c          298 g          226 t
ORIGIN      
        1 cagccgagga caattactcc accgtcacgc tcgcgtccgg cgaacgcgtt ctcctgcgca
       61 aaagcctgag cgcgtgggaa tccattttgc ccgcctcgca tttcgcccgc gtgcatcgca
      121 cccgcatcgt caacctcgcc cacgtgctcc gctacgaacg ccagggcgac ggcgcgctgc
      181 tctttatcaa gggcgccgcc gaacccgtgc ccgccagtcg ccgcacgtgg agtgacgtcc
      241 gcacgcgcct cgccgagctg catcctcagc tatagagcgt aatgaacttt cgagtggcga
      301 ccgtcagccg tagcgcggtc agcttgaccg cgttgatgcg cctcgaacac ggtcagattg
      361 accgggctac ccgcagacgt tcgacgccgc agctcatcgc ccccgctcgc cccaccggcc
      421 ccgcctttca cccccgcgcg ctggttgccg cgttgttttc gtcctcaacg tggcgcacac
      481 atgcctctgc ggttcgacga cacaatttcc ctgcgcaccg ctggtcgcgc gcatttcacc
      541 tcgctgcgcc agatcgtgct catcgccgcg cgcgataact acagcgaagt cactctttcc
      601 gacggcacgc gcgtcgtgct gcgcaagagt ttgaaagcat ggcagtcgct gttgcccgaa
      661 agtcatttcc tgcgcgtcca tcgcatgctg atcgtcaacc tcaccagcat cgtccgctac
      721 gagcgcgatc gcgacgagca cacccggctc ttcttcgccg gacgtgacac acccgtagcg
      781 gccagccgcc gcgcgtgggg gctcctgcgc cagcgcctcg cggatctgca ccccattcca
      841 taacttcgtt acaccgcagt gcatcggagc gagccggact ccgaccgcca ttcgccactc
      901 gggccccacc gctggcgact ttctcctcga atgccctttg aggccggcgc ctctctccac
      961 gagaccgccg gcttcacgcc gtcggctacg tttcacccat cctcatcgcc gtcgctcgac
     1021 cgcttccttc agctccatgc ataaaatcat cctcgccgtt ctcctcgccg ccacgccctt
     1081 gctctcgttc gcccaagtca attggtctcc cacccccggc tcggcgatta cgttcgccgg
     1141 ctcgtttgtt cgcac
//
LOCUS       DASPLT010000590         1286 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_294232, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000590 DASPLT010000000
VERSION     DASPLT010000590.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1286)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1286)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1286
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_294232"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..253)
                     /locus_tag="VEA63_06140"
     CDS             complement(<1..253)
                     /locus_tag="VEA63_06140"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009509697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit H"
                     /protein_id="HYD83609.1"
                     /translation="MSIAPSNFLEHAPLAARDWLVSIFPAPLHWLVHHLITIVAILAV
                     FGLFFALLTVVERKLLGRIQNRLGPNRAGLPFMKGKGPRL"
     gene            complement(351..1106)
                     /locus_tag="VEA63_06145"
     CDS             complement(351..1106)
                     /locus_tag="VEA63_06145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007363714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HYD83610.1"
                     /translation="MLGSGILKGLAVTAKNFAESYTTPERLVTVEYPEKQIPIPEAYR
                     NFPFLVTENATDPMGTLRCVACQICEKECPPQCIYIEKSKDKKPDATGKLQLYPAIFD
                     IDTSVCMSCQICVEVCPFDAIKMDQVFEIATTDRFTALLLNREQLAKPNSYYHDIHPT
                     EAAEVDARLAAERQAAAEKAKAAAAAKAALPKVATGVPTASASANTSTAASTSSTPTT
                     TPRPAATTPSNQPPAPTAEAPSAEAPKPPPPAA"
     gene            complement(1141..>1286)
                     /locus_tag="VEA63_06150"
     CDS             complement(1141..>1286)
                     /locus_tag="VEA63_06150"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012566.4"
                     /note="Catalyzes the transfer of electrons from NADH to
                     quinone; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HYD83611.1"
                     /translation="PNPYRYRVRPPSFVNLTVLEDMCLGHTLADAVVILGSIDIVLGE
                     VDR"
BASE COUNT          202 a          301 c          525 g          258 t
ORIGIN      
        1 agagccgcgg gcccttgcct ttcatgaaag ggaggccggc gcgattcggg ccgaggcgat
       61 tctggatgcg gccgaggagc ttgcgttcga ccacggtgag cagcgcgaag aagaggccga
      121 acacggcgag gatcgcgacg atcgtgatca ggtggtgaac gagccagtgc agcggtgctg
      181 ggaaaatgga tacgagccag tcgcgcgcgg cgagcggggc gtgttcgagg aagttggacg
      241 gcgcgatgct catcggcaga aaaggtaggg cgaaccgtcc cggtgagccg tggcggttgc
      301 ggctcggcgg ggacgcctcg ccctaccgtt gaaatcgtcg cgcgatccgg tcatgcggcg
      361 ggcggaggag gctttggagc ttctgcgctc ggcgcttcgg cggttggggc aggcggttga
      421 ttggagggag tcgtggcggc ggggcgcggt gtggtggttg gagtcgaaga ggtggacgcg
      481 gcggtgctgg tgttggcgga ggcggacgcg gtggggacgc ccgtggctac tttgggtaga
      541 gcggctttcg ctgcggcggc ggctttggct ttttcggcgg cggcctggcg ttcggcggcg
      601 aggcgggcgt cgacttcggc ggcttccgtg ggatggatgt cgtggtagta gctgttgggt
      661 ttcgcgagtt gctcgcggtt gagcagcaga gcggtgaagc gatcggtggt cgcgatctcg
      721 aaaacctggt ccatcttgat ggcgtcaaac gggcagacct cgacgcagat ctggcagctc
      781 atgcagacgg acgtgtcgat gtcgaagatg gcgggataca gttggagttt gccggtggcg
      841 tcgggcttct tgtccttcga tttttcgatg tagatgcact gcggcgggca ttctttttcg
      901 cagatctggc aggcgacgca gcggagcgtg cccattggat cggtggcgtt ttctgtgacg
      961 aggaacggga aatttcgata cgcctcggga atcgggattt gtttttccgg atactcgact
     1021 gtgaccaggc gctcgggcgt ggtgtagctc tccgcgaagt ttttcgcggt gacggcgagg
     1081 cctttgagta tgccggagcc tagcatcggg gatcggagat gggagatcgg agttcggggt
     1141 tcagcggtcg acctcgccga ggacgatgtc gatggagccg aggatcacga cggcgtcggc
     1201 gagggtgtgg ccgaggcaca tatcctcgag gacggtgagg ttgacgaacg agggcgggcg
     1261 gacgcggtaa cgatacgggt ttgggc
//
LOCUS       DASPLT010000591         1256 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_253017, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000591 DASPLT010000000
VERSION     DASPLT010000591.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1256)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1256)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1256
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_253017"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..231
                     /locus_tag="VEA63_06155"
     CDS             <1..231
                     /locus_tag="VEA63_06155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007361970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HYD83612.1"
                     /translation="ALKRAKEMAEAGVRSPDRLVAEATHVMSQRRRVRIIYVCIVDAT
                     TMEMMREVVPGRSMMVIAAWVDEIRLIDNLVL"
     gene            403..>1256
                     /locus_tag="VEA63_06160"
     CDS             403..>1256
                     /locus_tag="VEA63_06160"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HYD83613.1"
                     /translation="MPHGFCYLWSPKLLSMHVSSDVLIAASYVAIAITLLRIVRRRRD
                     GSFSGVGISFALFIVTCGFTHLLEAWNIWHADYWLSAWAKSVTAIASVGTAIYVFRLR
                     TAILILPTPRQVAALNAKLRSRSSELKQTENALRASEEQFRTAFDFAGIGMAVVGLDG
                     RWLRVNSRVCEITGYAEPELLATTVEAITVPEDTGRYREALDGLRVEAQRFFQLEKRY
                     RHKAGHSVAIRLTMALVRGPEGAPVHFVAQIEDVSDRKRLEEQLQHSRKMEAVGQLAG
                     GIAHDYNN"
BASE COUNT          216 a          336 c          441 g          263 t
ORIGIN      
        1 gccctgaaac gggcgaagga aatggctgag gcgggggtgc gcagcccgga tcgcctggtg
       61 gcggaggcga ctcacgtgat gagccagcgt cgccgcgtgc ggatcatcta cgtgtgtatc
      121 gtcgatgcca ctacgatgga aatgatgcgg gaggtcgtgc cgggccgcag catgatggtg
      181 attgccgcct gggtggacga gattcgtctg attgacaatt tggtgctctg agaagaagcg
      241 cccgacggag aacgcgtttc ggggcgtaag cggctctgct ggctgccgca ggattcgcac
      301 tgaccgtagc tcgcggcgaa tgggatttca gttcgccggc gaagcggacg attgaaggac
      361 tagaattctc tctcgtcgtt catgttgccg ctagcgtcgt tcatgcctca cggcttctgc
      421 tatctttggt cgccgaagct tctctccatg catgtctcga gtgacgtgct gattgcggct
      481 tcgtatgtcg cgatcgcgat cacgctgttg cgcatcgtgc gccggcgccg cgacggttcg
      541 ttcagcggag tcggtatcag cttcgcgttg ttcatcgtga cgtgcggctt cacgcacttg
      601 ctggaggcgt ggaatatctg gcacgcggat tactggttgt cggcgtgggc gaaaagtgtg
      661 acggcgatcg cctccgtggg gacggcgatc tacgttttcc gactgcgcac tgcgattctg
      721 attttgccga cgccgcgaca ggtcgctgcg ctcaatgcga aactgcggtc gcgttcctcg
      781 gagctgaagc agacggagaa tgcgctgcgg gcgagcgagg agcagtttcg aacggctttc
      841 gacttcgccg gcatcggcat ggcggtcgtg ggcttggatg gccgctggtt gcgcgtaaat
      901 tcgcgagtgt gcgagatcac cggctatgcg gaaccggagc tgctggcgac gacggtggaa
      961 gcgatcactg ttccggagga taccgggaga taccgcgagg ctctcgacgg gttgcgcgtc
     1021 gaggcgcagc ggttcttcca gttggagaaa cgttatcggc acaaggcagg ccacagcgtc
     1081 gcgatccgcc tgacgatggc gctcgtgcga ggtcccgaag gtgcgccggt gcacttcgtg
     1141 gcgcagatcg aagatgtctc tgatcgcaag cgcctcgagg agcagttgca gcactcgcgc
     1201 aagatggagg cggtcggcca gctcgcgggc ggcatcgcgc atgattataa caacat
//
LOCUS       DASPLT010000592         4087 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_150364, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000592 DASPLT010000000
VERSION     DASPLT010000592.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 4087)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4087)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4087
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_150364"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1044)
                     /locus_tag="VEA63_06165"
     CDS             complement(<1..1044)
                     /locus_tag="VEA63_06165"
                     /inference="COORDINATES: protein motif:HMM:NF015331.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HYD83614.1"
                     /translation="MKDSHFSLTTRIERPAPEVFAWHERPGALARLCPPWERVEIMEA
                     SGGVRDGARVRVRTKIGPVWSEWWVEHRDYVEGAQFRDVQVSGPFAKWEHVHRFRPDE
                     ANANACWLTDEITYRLPGGAIGRVLSGEFVRRRLERLFRWRHATTKADVEGRRVTAKR
                     ILIAGASGLIGRALTPFLQTQGHTVLRLVRRKPVSAGDIEWNPAKRELSSEVVEGMDA
                     IVNLSGANVGAGRWTRRRREEIVTSRVNATATLVRAMERLARKPEVFVSMSAAGFYGE
                     RGNERLTEEAASGAGFLAEVCRSWEAEALAAERLSVRTTRLRFGVVLTPAGGALAKLL
                     PLFRAGVGGRVGDG"
     gene            complement(1109..2551)
                     /gene="glgA"
                     /locus_tag="VEA63_06170"
     CDS             complement(1109..2551)
                     /gene="glgA"
                     /locus_tag="VEA63_06170"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HYD83615.1"
                     /translation="MKIVHVASELFPYVKTGGLADAVSSLAGALADSGHEVSVFLPGY
                     RAALEHPHAANAQRRLRLKIEMGSQFLSGDVRVYSPRKNLTVHLICREEFFDRRAPYG
                     NGERDYEDNADRFIFFCKGVAETLRLADMQADVVHAHDWQAALVPLLLRDTERRQSLT
                     LAVRTIFTIHNIAFQGVFPRSTFARTNLPEELNSVDGLEYYEQINFMKAGLLFADRVT
                     TVSPRYAQEIQTPEFGCGLEGVVQTRADDIVGLLNGVDTHVWNPATDELLPMRYSAAN
                     MSGKRTCREELLQRAGFDAGFTGPVFGMVARLTEQKGVDLLLANQDFFVKEDCRLVIL
                     GSGERRYEEALRALATRVPNKVALTAKLDEAMSHLIEAGSDFFVMPSLFEPCGLNQMY
                     SQLYGTVPIVSRVGGLADTVTDADEQPETGTGLMCEPNAASLRDALGRALKLFANAPR
                     YSAVQQRAMARNFSWSVAAVGYETLYRESL"
     gene            2629..>4087
                     /locus_tag="VEA63_06175"
     CDS             2629..>4087
                     /locus_tag="VEA63_06175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNB domain-containing ribonuclease"
                     /protein_id="HYD83616.1"
                     /translation="MTLRERLLALLREPSYSPANESELLRKLGLHKKRRASLAHEIRQ
                     LLAKGEAVRSGDDRIRIPREERAPKKEKVGDRKLFTPTRRAAATPPVDAPAPSPTAKR
                     SAQRAPAAARAPSPTRSANANEMIGRIQFRAGGSAFVVPENPETGLSDLGEPSVQIFP
                     EDTDIALPGDRVLVRIHAGRRGKRPGERIGGVERILERGRESIVGDLRRSGRDYIVVP
                     DDPRFVYEIYVADPARGTLRPAPQPGDKVVVQLDDWKHRRDRITGEITKHLGRTHEPR
                     AELLGIFEKYNLSTSFPADVEREAAELPDRVPASALAHRLDYREKAVFTIDPDDAKDF
                     DDALSYEVLDGGDVRIGIHIADVSTYVHAGTALDREAQRRGNSTYLVGVVIPMLPEKL
                     SNGLCSLVEGQDRLCRAVFLTFAKNGRIKDTSFANTVIRSRKRLTYKQAYALLFENDL
                     DRVRALPLPPKHQTGSTGRALNSLSDAELVDLQSWV"
BASE COUNT          754 a         1563 c         1061 g          709 t
ORIGIN      
        1 tccgtctccc acgcgcccac ccacgcccgc gcgaaacagc ggcaatagtt tcgcgagcgc
       61 cccgccggca ggcgtcagca ccaccccgaa acgcagtcgc gtcgtgcgca cactgagacg
      121 ttccgccgcc aacgcctccg cttcccaact ccggcacacc tccgccaaaa aacccgcgcc
      181 gctcgccgct tcctccgtca gtcgttcgtt gccacgctct ccataaaagc ccgccgccga
      241 catgctcacg aagacctcgg gttttcgcgc gagccgctcc atggcgcgca caagcgtcgc
      301 tgtcgcattc acccgactcg tcacgatttc ttcccgccgt cttcgcgtcc aacggccggc
      361 gcccacgttt gcccccgaca agttcacgat cgcgtccatg ccttcgacca cctcgctgct
      421 cagttcccgc ttcgccgggt tccactcgat gtctcccgcg ctcaccggct ttcgccgcac
      481 gagacgcaac accgtgtggc cttgcgtctg caaaaatggc gtcagcgcgc gcccaatcag
      541 tcctgatgcg ccagcgatca gtatccgttt cgcggtgacg cgccggcctt caacatccgc
      601 tttcgtcgtc gcatgccgcc agcgaaacag ccgttccagc ctccttcgca caaattctcc
      661 gctcagcaca cgcccgatcg ccccgcccgg cagccgatag gtgatttcat ccgtcaacca
      721 gcacgcattc gcattggctt cgtccggccg aaatcgatgc acatgctccc acttcgcaaa
      781 cggcccgctc acctgcacgt cgcgaaactg cgcgccttca acgtagtcgc gatgctccac
      841 ccaccattcg ctccacacag gtccgatctt cgtgcgaacc cgcacacgcg ctccgtcgcg
      901 caccccaccg ctcgcttcca taatctccac acgttcccac ggcggacaca accgcgcgag
      961 cgcccccggc ctctcatgcc aggcaaacac ctccggcgcc ggccgctcga tccgcgtcgt
     1021 cagcgagaaa tgactgtcct tcataaccga gccgctccgc tacgccgcgc acgtccctcc
     1081 gttctcaccg catccgtcgt cccacgcttc acaacgattc gcgatacagc gtctcgtagc
     1141 ccaccgccgc cacgctccag ctaaaattcc gcgccatcgc gcgctgctgc accgccgaat
     1201 aacgcggcgc attcgcgaag agcttcagcg cccgcccgag ggcatcgcgc agcgacgcgg
     1261 cattcggttc acacatcagc cccgtgcccg tctccggctg ctcgtcggca tccgtcaccg
     1321 tgtccgccaa ccctcccacg cgactcacga tgggcacggt gccgtaaagc tgcgaataca
     1381 tctggttcaa accgcacggc tcgaacagcg agggcatcac aaaaaaatcg ctgcccgctt
     1441 cgatcaggtg gctcatcgct tcgtcgagct tcgccgtgag cgcgaccttg ttcggcacgc
     1501 gcgtcgcgag cgcgcgcaac gcctcctcat aacgccgttc gcccgagccg aggatcacga
     1561 ggcggcagtc ctccttcacg aaaaaatcct ggttggccag gagcagatcc acgcctttct
     1621 gctcggtgag tcgcgcgacc atgccgaaca ccggcccggt aaatcccgca tcgaaacccg
     1681 cccgttgcag cagctcctcg cgacacgtcc gctttccgct catgttcgcc gccgaatagc
     1741 gcatcggcag caactcgtcc gtcgccggat tccacacgtg cgtgtcgacg ccgttgagca
     1801 gtcccacgat atcgtccgcc cgcgtctgca ccacgccttc gagcccgcag ccgaactccg
     1861 gtgtctggat ttcctgcgcg taacgcgggc tcaccgtcgt cacgcgatcg gcgaacagca
     1921 gccccgcctt catgaaattg atctgctcgt aatactccag accgtccacg ctgttgagtt
     1981 cctccggcag attcgtgcgg gcgaacgtgc tccgcggaaa cacaccttgg aacgcgatgt
     2041 tgtggatcgt gaaaatcgtg cgcaccgcaa gcgtgagcga ttgccgccgc tccgtgtcgc
     2101 gcaacaacaa cggcaccagc gccgcctgcc agtcgtgcgc atgcaccacg tccgcctgca
     2161 tgtccgccag acggagcgtc tcggccacgc ctttgcagaa aaaaatgaag cggtccgcgt
     2221 tgtcctcgta atcgcgttcg ccatttccgt aaggcgcgcg ccggtcgaag aattcttccc
     2281 ggcagatgag atgcaccgtc aggtttttcc tcggtgaata aacccgcaca tcgcccgaga
     2341 gaaactggct gcccatctcg atcttcaacc gcagtcgtcg ctgcgcgttc gccgcatgcg
     2401 gatgctcgag cgccgcgcga tatcccggaa ggaaaaccga aacttcgtga ccgctgtccg
     2461 cgagcgcgcc ggccaacgaa ctcaccgcgt ccgccaagcc acctgtcttg acgtagggga
     2521 acaattcact ggcgacgtgg acgattttca tcgcgccaac ctatgtcccg ctctttcgtt
     2581 aggccaactc caaagcacgg cttgcgcgcc gcggcccggg atactcacat gacacttcgc
     2641 gaacgcctgc tcgccctttt gcgcgagccc agttactcac ccgccaacga atccgaattg
     2701 ctccgcaagc tcggccttca caaaaagcgt cgcgcttcgc tcgcgcacga aatccgccag
     2761 ttgctcgcca aaggcgaagc ggttcgctcg ggcgacgatc gcatccgcat cccgcgcgaa
     2821 gaacgcgcgc cgaaaaaaga aaaagtcggc gatcgcaaac ttttcacgcc cacccgacgc
     2881 gccgccgcga ccccgcccgt cgacgcgccc gcgccgagcc cgaccgccaa gcgctcggct
     2941 caacgcgcgc ccgccgccgc gcgtgctccg agtccgacac gttcggcgaa cgccaacgag
     3001 atgattggcc gcatccagtt tcgcgccggc ggctccgctt tcgtcgttcc cgaaaatccc
     3061 gaaaccggtc tctccgatct cggcgaacca tccgtgcaga tttttccgga ggacaccgac
     3121 atcgcgcttc ccggcgaccg cgtgctcgtt cgcatccacg ccggccgccg cggtaaacgc
     3181 cccggcgaac gcatcggcgg cgtcgaacgc attctcgagc gcggtcgcga gtccatcgtc
     3241 ggcgatctgc gccgcagcgg tcgcgattac atcgtcgtgc ccgacgatcc gcgtttcgtt
     3301 tacgaaatct acgtcgccga ccctgcgcgc ggaacgctgc gtcctgcacc gcaaccgggc
     3361 gacaaggtcg tcgtgcagct cgacgattgg aaacaccgtc gcgaccgcat caccggcgaa
     3421 atcaccaaac acctcggtcg cacgcacgag ccgcgcgccg aactgctcgg cattttcgaa
     3481 aaatacaatc tctcgaccag cttccccgcc gacgtcgaac gcgaggccgc cgagcttccc
     3541 gatcgcgtgc ccgcgagcgc cctcgcccac cgcctcgact accgcgagaa agccgtcttc
     3601 accatcgatc ccgacgacgc gaaggatttc gacgacgcgc tttcttacga agtgctcgat
     3661 ggcggcgacg tccgcatcgg catccatatt gcggatgtgt cgacctacgt ccacgccggc
     3721 actgcgctcg atcgcgaagc gcagcgccgc ggcaattcca cgtatctcgt cggcgtcgtc
     3781 attccgatgc tgccggaaaa attgtccaac ggcctctgct cgcttgtcga aggccaggac
     3841 cgtttgtgca gggcggtctt tctcaccttc gcgaaaaacg gccgcatcaa ggacacctct
     3901 ttcgcgaaca ccgtcatccg cagccgcaag cgtctcactt acaagcaagc ctacgcgctg
     3961 ctcttcgaaa acgatctcga ccgcgtccgc gcgctcccgc tgccgccgaa acatcagacc
     4021 ggctcgaccg gccgcgcgtt aaactccctc agcgacgcag aactcgtcga cctgcaatcc
     4081 tgggttc
//
LOCUS       DASPLT010000593         1512 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_253041, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000593 DASPLT010000000
VERSION     DASPLT010000593.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1512)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1512)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1512
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_253041"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..856
                     /locus_tag="VEA63_06180"
     CDS             <1..856
                     /locus_tag="VEA63_06180"
                     /inference="COORDINATES: protein motif:HMM:NF021606.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2339 domain-containing protein"
                     /protein_id="HYD83617.1"
                     /translation="YQIVKTTWPNAIMGALPAAFALAPAASLIAIARASPPSDAPSAR
                     VNQLAWFGGVALLFITLIFPIQFERQWITVAWALEGAALLLLFRRVPHPGLRATGIAL
                     LVVAFARLALNRAVFAYHERGDVPLLNWYLYAYGIAIACLFVGARFFAPGERVLRINA
                     PALLNTLGTILAFLLLNIEIADYFSAPGATLTFDYSGNFARDVAYTVGWAAFALVLLG
                     AGIWKKTRTARFAAIALLGVTLLKLFFHDLAELGQLHRVGALVAVAIVAIAASFLYQR
                     FAPSDHRR"
     gene            complement(853..1050)
                     /locus_tag="VEA63_06185"
     CDS             complement(853..1050)
                     /locus_tag="VEA63_06185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD83618.1"
                     /translation="MDDPSDHAPVDRVGVGGRGRPQDEFGALPHFRGRDGESTDADVR
                     AGHGGDDYTTTRDVFSIERPD"
     gene            1265..>1512
                     /locus_tag="VEA63_06190"
     CDS             1265..>1512
                     /locus_tag="VEA63_06190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE3 domain-containing protein"
                     /protein_id="HYD83619.1"
                     /translation="MNLSIEKKVVTGFSVAVLVLLLVAGVVAWNARRFGETFRAVDRA
                     HQVLRHLEDVMLGTLSVQTSARGFALTGAEEFLPRLENG"
BASE COUNT          235 a          536 c          437 g          304 t
ORIGIN      
        1 ttatcaaatc gtgaaaacca cgtggcccaa cgccatcatg ggcgcgttgc cagccgcctt
       61 tgccctcgca ccggccgcga gcttgattgc catcgcgcgc gcttcgccac catccgacgc
      121 gccgtcggcg cgagtcaacc agctggcgtg gttcggcggc gtcgcgctgc tttttatcac
      181 gctgatcttc cccattcaat tcgagcggca atggatcacc gtcgcgtggg cgctcgaggg
      241 cgcggcgctg ctgctgctgt ttcgccgcgt gccgcatccg ggtctgcgcg cgaccggcat
      301 cgcgctgctc gtcgtggcgt tcgcgcggct ggcgttgaac cgcgcggtgt tcgcgtatca
      361 cgaacgtgga gacgttcccc ttctcaactg gtatctctac gcctacggca ttgcgatcgc
      421 gtgcctgttc gtcggcgcac gcttcttcgc gcccggcgaa cgcgtgctgc ggatcaacgc
      481 ccccgcgctg ctgaacaccc tcggcacgat cctcgccttt cttctgctca acatcgagat
      541 cgcggattat ttcagcgcgc ccggcgccac cctgacattc gactactccg gcaattttgc
      601 gcgcgacgtg gcttacacgg tcggctgggc cgccttcgcc ctcgtgctgc tcggcgccgg
      661 catctggaaa aaaacgcgca cggcccgctt cgccgcgatc gcgcttctcg gagtcacgct
      721 gctcaagctc ttcttccacg atctcgccga gttgggtcaa ctgcaccgcg tcggcgcgct
      781 cgtcgccgtc gccatcgtgg ccatcgccgc gtccttcctc tatcagcgtt tcgccccgtc
      841 cgatcaccga cgctagtccg gccgctcgat cgaaaacacg tcgcgcgttg tcgtataatc
      901 gtcgccgccg tgaccggcac gaacatcagc gtcggtggat tcgccgtcgc gcccccgaaa
      961 atgcggaagg gcgccaaatt cgtcctgcgg tctcccccga ccgccgacac ccacgcgatc
     1021 gaccggggca tgatcgctcg gatcatccat tgataagcat cgcgcggaca gttcggagaa
     1081 atcgaattgc atcgcacggc cgcaaatgcc gcggacgtgc aacgtagcgc caaatcgccg
     1141 gtccctacaa gacccgcgcg gctgactccg cgctggcgca atttaccgtt gtcggtttgt
     1201 tgagaaacat tcccacccgc cgcactccta tcctgcgaca gccctgttgt tttcagaacg
     1261 ccgcttgaac ctctcaatcg aaaaaaaagt cgtcaccgga ttctcggtcg ccgttctcgt
     1321 gctgctgctg gtcgccggcg tggtcgcttg gaatgcacgg cgcttcggcg agacgttccg
     1381 cgccgtcgat cgcgcgcacc aggttctcag gcacttggaa gacgtgatgc tcggcaccct
     1441 cagcgtgcag accagcgcgc gaggcttcgc gctgaccggc gcggaagaat ttctcccgcg
     1501 gctcgagaac gg
//
LOCUS       DASPLT010000594         1148 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_231945, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000594 DASPLT010000000
VERSION     DASPLT010000594.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1148)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1148)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1148
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_231945"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..286
                     /locus_tag="VEA63_06195"
     CDS             <1..286
                     /locus_tag="VEA63_06195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD83620.1"
                     /translation="NLLTAYHFLKEIETARQAGDPALVAQKESDYHALLREDVEVRTR
                     FIALLSRFSTLTPVLTRTSLSEQGIARQVDYMKAEIEKTNAYLAQHAGTP"
     gene            283..>1148
                     /locus_tag="VEA63_06200"
     CDS             283..>1148
                     /locus_tag="VEA63_06200"
                     /inference="COORDINATES: protein motif:HMM:NF015604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046559 - alpha-glucuronidase activity
                     [Evidence IEA];
                     GO_process: GO:0045493 - xylan catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucuronidase family glycosyl hydrolase"
                     /protein_id="HYD83621.1"
                     /translation="MKHPAPPPLPLIRPARIVCGWLLALAAVSSPAFEYDRSIGQIAH
                     LPVTLVEKPFVLRFSPEELLAQPVWSKRIDGIVLSNLADAPTRTGARELQNGLVAAKL
                     GDAPLIEIEPSLTELPRELAGKHVVVLGRPDELALVRTLVDEQKLTVTDERLNGDGFI
                     VKPLKKWDAHLLLVTSCVSRGVMYGAFELEERSTSRGVPRIDESFVPAVRDRAWSFWN
                     FYPEPPGLIGRSRYNHSIHYFGDWPGILAYPAYPELGGEKNVSRRLERQTQIHRMLGD
                     ATKYGATPGLIF"
BASE COUNT          211 a          442 c          296 g          199 t
ORIGIN      
        1 caacctgctc acggcctatc atttcctcaa ggaaatcgag acggcgcggc aggccggcga
       61 tcccgcgctc gtggcgcaga aggaaagcga ctaccacgca ttgctccgcg aggacgtcga
      121 ggtccgcacg cgcttcatcg cgctcctctc ccggttctcg accctgactc ccgtcctcac
      181 gcgcacgagc ctttccgagc agggcatcgc ccgccaagtc gattacatga aagccgaaat
      241 cgaaaagacc aacgcctacc tcgcacagca cgccggcacc ccgtgaaaca tcccgcacct
      301 cccccgctcc cgctcattcg acccgcccgg atcgtctgcg gctggctcct cgccctcgca
      361 gctgtatcgt ctccggcgtt tgaatacgac cgctccatcg ggcagatcgc ccacctgccc
      421 gtcacgctcg tggaaaagcc atttgtcctg cgcttcagtc cggaggaact gctcgcgcag
      481 ccggtctggt cgaaacgcat cgacggcatc gttctcagca acctcgccga tgcgccgacc
      541 cgcaccgggg cgcgggagct tcaaaacggc ctcgtcgcgg cgaagctggg cgacgctccc
      601 ctcatcgaaa tcgagccgtc gctgaccgaa ctcccgcgcg aactcgccgg caaacatgtc
      661 gtcgtcctcg gccgccccga cgaactcgct ctcgttcgca ccctcgtcga cgagcaaaaa
      721 ctcaccgtca ccgacgaacg cctcaacggc gatggcttca tcgtcaagcc gctgaaaaag
      781 tgggatgccc atctcctcct cgtcacgtcc tgcgtcagcc gcggcgtcat gtatggcgcc
      841 ttcgaactcg aagaacgctc cacctcgcgc ggcgtccctc gcatcgacga atcgttcgtg
      901 cctgcggtcc gcgatcgcgc ctggtcgttt tggaattttt accccgagcc gcccgggctc
      961 atcggccgct cgcgctacaa ccactcgatc cattacttcg gcgactggcc gggcattctc
     1021 gcctaccccg cctatcctga actcggcggc gaaaaaaacg tttcccgccg cctcgagcgc
     1081 cagacccaaa ttcatcgcat gctgggcgac gccacgaaat acggcgcgac ccccggcctc
     1141 atcttcaa
//
LOCUS       DASPLT010000595         1475 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_253076, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000595 DASPLT010000000
VERSION     DASPLT010000595.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1475)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1475)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1475
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_253076"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..292)
                     /locus_tag="VEA63_06205"
     CDS             complement(<1..292)
                     /locus_tag="VEA63_06205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD83622.1"
                     /translation="MSTHSEHRATAYPASLRAVELAAIAARRRSAGTDPDAPIVGFAL
                     SGGGIRSATFCLGFFQALARQRLVRRIDLLSTVSGGGYFGSFLGTAFSRDDGP"
     gene            complement(578..976)
                     /locus_tag="VEA63_06210"
     CDS             complement(578..976)
                     /locus_tag="VEA63_06210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5056 domain-containing protein"
                     /protein_id="HYD83623.1"
                     /translation="MEPTDLNPLSRDDERLSAILREAQPAIADDGFSTRVLAALPPPK
                     NSAHPLLSRPFACAIGTLVGIAVAWNHGVFSASFTSAFADLRSSVSTSLATNADMPAS
                     PGLALALALAVTGASLFYAFGFTSARVRRP"
     gene            complement(958..>1475)
                     /locus_tag="VEA63_06215"
     CDS             complement(958..>1475)
                     /locus_tag="VEA63_06215"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HYD83624.1"
                     /translation="IARVLASDDRAAFGELVRRHQSSVRQFLRYLTRGDVATADDLAQ
                     ETFVHAYRSLARFRGDASFSTWLLGIGRNYWRNAQRRDLRRDQLVEPMPDVAAPTATH
                     TADLRHDLTLALRELTTDEQLAIHLGYQEGKSHSEIAALLDCPLGTVKTHLARAKEKL
                     RHLLAAWNPQI"
BASE COUNT          295 a          391 c          566 g          223 t
ORIGIN      
        1 gcgggccgtc gtcgcgtgaa aaagccgtgc cgagaaaact gccgaaatag ccgccgccgg
       61 agacggtcga aagcagatcg atgcggcgga ccaggcgctg gcgggcgagc gcctgaaaaa
      121 agccgaggca gaatgttgcg ctgcgaatgc cgccgccgga gagcgcgaag ccgacgatcg
      181 gcgcgtcggg atcggtgccg gcgctgcggc gacgcgcggc gatggcggca agttccacgg
      241 cgcggagcga agcggggtaa gccgtggcgc ggtgttcgga gtgcgtggac acgggcggcg
      301 gccatgttga tgaggagcga gccgcgaaaa taccagcgaa cgggacgacg gaagttcccg
      361 aatgtgggga cggggaaaat caggtggacg cgcggatttt cgattcagga ttttggattt
      421 tcgatttcgg acgaccggcg ggagcagatc gaaaatcgag agtcgcgtgg gcggatcacc
      481 gcgaggtgga gcgcgacgtc cttgcccgac tgttagcgag cgagacgtta ccaagctgga
      541 caggcacgtc gggctccacc tgctgcacgt gttcgactca ggggcggcga actctcgcag
      601 aggtaaagcc gaaggcgtag aacagcgaag cgccggtgac tgcgagcgcg agggcaaggg
      661 cgaggcccgg actcgctggc atgtcggcgt tggtcgccag cgaggtgctg acagatgaac
      721 ggagatcggc gaacgccgag gtgaacgaag cggagaaaac gccgtggttc cacgcgacgg
      781 caatgccgac cagcgtgccg atcgcacacg cgaaaggtcg cgacagcagc gggtgcgccg
      841 agttttttgg cggcggaaga gccgccagaa cacgcgtgga aaaaccatcg tcggcgatcg
      901 cgggctgcgc ctcgcgcaag atcgccgaca gccgttcgtc gtcgcgagag agaggattta
      961 aatctgtggg ttccatgcgg cgaggaggtg gcggagtttt tctttcgcgc gagcgaggtg
     1021 agttttcacc gtgcccaacg ggcagtcaag aagcgcggcg atttcgctgt gcgacttgcc
     1081 ttcttgatag ccgagatgaa tcgcgagttg ttcgtcggtt gtgagctccc ggagcgcgag
     1141 cgtgaggtcg tggcgcaagt cggccgtgtg agtcgcggtc ggagcggcaa catccggcat
     1201 gggttcgacg agttgatcgc ggcggagatc gcggcgttgt gcgttgcgcc agtaatttcg
     1261 cccgatgccg agcagccagg tggagaaact cgcgtcgcca cggaaacgag cgagagagcg
     1321 gtaggcgtgg acgaacgttt cctgcgcgag gtcgtcggcg gtcgcgacgt cgccgcgggt
     1381 gaggtagcgg aggaactgac ggacggaaga ttgatgccgc cgcacgagtt cgccgaaagc
     1441 ggcgcggtca tccgaggcga gcacccgcgc gatca
//
LOCUS       DASPLT010000596         1130 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_150403, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000596 DASPLT010000000
VERSION     DASPLT010000596.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1130)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1130)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1130
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_150403"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1130
                     /locus_tag="VEA63_06220"
     CDS             <1..>1130
                     /locus_tag="VEA63_06220"
                     /EC_number="1.1.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009508783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HYD83625.1"
                     /translation="SDPIIFGHVVRVFFKDLLAKHADTFAALGVDLNNGLGDLLSKIA
                     TLPADQRAAIEADIAATYAAGPALAMVNSDKGITNLHVPSDVIVDASMPAMIRTSGQM
                     WNAQGKQQDTLAVIPDSSYAGVYQTVIDYCKKHGAFDPKTMGSVPNVGLMAQAAEEYG
                     SHNKTFQMSANGTVRVTDASGKVLLEHAVEEGDIWRACQTKDAPVQDWVKLAVNRARL
                     SNTPAIFWLDEKRAHDAQIIAKVKTYLAQHDTTGLDIKILAPAAACQATLERLVKGQD
                     TISVTGNVLRDYLTDLFPILEVGTSAKMLSIVPLMNGGGLFETGAGGSAPKHVQQFTE
                     ENFLRWDSLGEFFALAASLEHLSIVTKHAKAKVLADTLDAAN"
BASE COUNT          240 a          394 c          311 g          185 t
ORIGIN      
        1 tctccgaccc gatcatcttc ggccacgtcg ttcgtgtctt cttcaaagac ctgctcgcga
       61 aacacgccga cacgttcgcg gccctcgggg tcgatttgaa caacggcctc ggcgatctcc
      121 tgagcaaaat cgcgacgctt cccgccgacc agcgcgccgc gatcgaagcc gacatcgccg
      181 ccacctacgc cgccggcccc gcgctcgcga tggttaactc cgacaaaggc atcaccaatc
      241 tccacgtgcc gagcgacgtc atcgtcgacg cgtcgatgcc cgcaatgatc cgcacgtccg
      301 gccaaatgtg gaacgcgcaa ggcaagcaac aggacacgct cgccgtcatc cccgacagca
      361 gctacgcggg tgtttaccag accgtcatcg actactgcaa aaaacacggc gcgttcgatc
      421 ccaagacgat gggctccgtc cccaacgtcg gcctcatggc ccaagccgcc gaagaatacg
      481 gctcgcacaa caagacgttc caaatgtccg cgaacggcac cgttcgtgtc accgacgcct
      541 cgggcaaagt tctgctcgaa cacgctgttg aggaaggcga catttggcgc gcctgccaga
      601 cgaaggacgc gccggtgcag gattgggtga agctcgccgt caaccgtgcg cggctctcga
      661 atacgcctgc gattttctgg ctggacgaga aacgcgcaca cgacgcgcag atcatcgcga
      721 aagtgaaaac gtatctggcg cagcacgaca ccaccggcct ggatatcaag atcctcgctc
      781 ccgccgccgc ctgccaagcc acgctcgagc gtctcgtcaa aggtcaggac acgatcagcg
      841 tcaccggcaa cgttctccgc gactacctca ccgatctttt cccgatcctc gaagtgggaa
      901 cgagtgcgaa gatgctctcc atcgttccgc tcatgaacgg aggcggcctg ttcgaaaccg
      961 gcgccggcgg ctctgcaccg aagcatgtgc agcagttcac cgaggagaac tttctccgct
     1021 gggacagcct cggcgaattc ttcgcgctcg ccgcgtcgct ggagcacttg agcatcgtga
     1081 cgaagcacgc caaggccaag gtgctcgccg acacgctcga cgccgcgaat
//
LOCUS       DASPLT010000597         3172 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_273066, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000597 DASPLT010000000
VERSION     DASPLT010000597.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 3172)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3172)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3172
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_273066"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1118)
                     /locus_tag="VEA63_06225"
     CDS             complement(<1..1118)
                     /locus_tag="VEA63_06225"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HYD83626.1"
                     /translation="MIRGGKQIRIALSVAVVIALASAGGWWWRAAATRDRVAAIIPTQ
                     PDRLRAASELQARVNEAEARARGLRQPLDGLADLARLYHANGFAAQAAECARALMQLE
                     PSNALWPYLQAHMMGGYGDLEGALPLLQRAIELAPNYAPARVRLGDALLKQNEAERAA
                     QTYRDALSRDPGNGYASIGLARVEIAAGNETAARDRLLKLVAAQPMFAPAWGLLIGID
                     ERLGDRTAAETHRLHARNAGTSREMPDPWIDELMAYCYDAYQLAVAAAAADPMNNQAR
                     ARQLLERAVSVAPNDDLPRRLLGNLLSDLGELSSARLFLEQATVFAPKEPDNWSHLVR
                     VLKAMGDISAASRAVDVGLEHCPDSAVLHLERGRRLAAA"
     gene            complement(1115..2236)
                     /locus_tag="VEA63_06230"
     CDS             complement(1115..2236)
                     /locus_tag="VEA63_06230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HYD83627.1"
                     /translation="MSGKPWITHVNALDLDDDGRCDIVVCDAKANTVSWLRQTASGRF
                     VETVLAPDIAAPVRAEAVDIDGDGDLDLLIASMGQVFPNNDKIGAVIILENEGDSHFA
                     KHVIVDGIARVTDVRAADFDGDRRLDLAVGQFGYEQGEIRWMKNLGGWRFESRILLSL
                     SGTVNVCAADLTGDGHNDIVALVSQQWEEIHLFEGDGNGNFARRVIFGSTNQDFGSSG
                     MALSDLDRDGRLDILYTNGDGFDYAQPGPRPWHGVQWLENGGNGTFKNRRIGALEGAY
                     SPVAVDLDRDGDMDVIAVSGFNEWNQPHVASLMWFKNDGRMGFTPHVLARAPTHLLTC
                     VAADFDGSGNPALVTGAFHAYPPWDRMSRLLLWEHAESP"
     gene            2591..>3172
                     /locus_tag="VEA63_06235"
     CDS             2591..>3172
                     /locus_tag="VEA63_06235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HYD83628.1"
                     /translation="MRKIWKSRRFIPALALLCSVSGVGQEHPGVDRQPLPTRARRTGP
                     ALFTTLPPEQTGIDVINRYDDVRIWGDRYEEFAFGSIGTGVAIADYDADGRPDVFLAG
                     KSAGGRLYRNLGDWKFRDVTEPSGLIAPDAGWQAGAAFVDLDNDGDLDLYICRANEPN
                     LLYVNQGDGTFKEGAKAAGLDVVDASVMAAFADY"
BASE COUNT          590 a         1073 c          951 g          558 t
ORIGIN      
        1 gcggccgcga gtcgccgacc gcgctcgagg tgcaggaccg cggagtccgg acagtgttcg
       61 agcccgacgt cgaccgcccg actcgccgcg gagatgtccc ccatcgcctt gagcacccgg
      121 acgagatgac tccagttgtc cggttccttt ggtgcaaaca ccgttgcttg ctcgagaaac
      181 aatctcgcac tcgaaagctc ccccagatcg gacaggagat tgccgaggag gcggcgcggc
      241 aaatcgtcgt tgggcgcgac gcttactgcc cgctcaagta actgccgcgc ccgggcctga
      301 ttattcatag ggtcggccgc cgccgcagcc accgccaact gatacgcgtc atagcaatac
      361 gccatcaact catcgatcca agggtccggc atttcccggg acgtgccggc attgcgagcg
      421 tgcaagcgat gggtttccgc ggccgtccgg tcgcccagcc gctcgtcgat gccaatcagc
      481 aagccccagg ccggtgcgaa catcggctgc gcggcgacga gtttcaacag ccgatcgcgc
      541 gctgccgttt cattgccggc cgcaatctcc acccgcgcaa gtccgatcga cgcgtagcca
      601 tttcccggat cgcgcgaaag cgcatcgcgg taggtttggg cagcccgttc ggcttcgttt
      661 tgctttagca gcgcatcccc aagtcggacg cgtgccggcg catagttcgg cgccagctcg
      721 attgctcgct gcagcaaagg taacgcgcct tcgagatctc cataaccgcc catcatgtgc
      781 gcctgcaggt aaggccacaa cgcgttcgaa ggctcgagct gcatcagcgc gcgggcgcat
      841 tcggcggctt gcgccgcgaa tccgttcgcg tgatacaacc gggccaggtc ggccagccca
      901 tccaacggtt gccgcagacc gcgcgcacgc gcttcggctt cgttgacccg cgcctgtaat
      961 tccgaagcgg ctctgagccg atcaggctgc gtcgggatga tcgccgcgac cctatcgcga
     1021 gtagcggccg cacgccacca ccagccgccg gcgctcgcca gcgcaatcac cacagcaacg
     1081 ctcagtgcga tccggatttg ctttccgccg cgtatcatgg cgactccgcg tgctcccaca
     1141 gcagcagccg actcatgcga tcccacggcg gatacgcatg aaaggcgccg gtgaccagcg
     1201 ccggatttcc cgacccatcg aaatccgccg cgacacaggt gagcagatga gtgggcgcac
     1261 gcgccaaaac gtgcggggtg aaacccatgc ggccatcgtt cttgaaccac atcagcgacg
     1321 ccacatgcgg ttggttccac tcgttgaacc cgctgactgc gatcacatcc atgtcgccat
     1381 cccgatccaa gtccacggca accgggctgt aggcgccctc aagcgcaccg atgcgccgat
     1441 tcttgaaagt tccgttaccg ccgttctcga gccattgcac cccgtgccat gggcgcggac
     1501 ccggctgcgc ataatcgaag ccgtcgccgt tggtatagag aatgtccagc cggccatcgc
     1561 gatcgagatc gctcaacgcc atgccgctgc tgccgaagtc ctgattggtg gaaccgaaaa
     1621 tgacgcgcct cgcgaagtta ccgttcccgt ccccttcgaa cagatggatc tcttcccatt
     1681 gctgcgatac cagcgcgaca atgtcattgt gcccgtcgcc ggtcaggtcg gctgcgcaga
     1741 cgttcaccgt gcccgaaaga ctgagcagga ttcggctttc gaaccgccag ccgcccaggt
     1801 ttttcatcca gcgaatctcg ccttgctcat agccgaactg gcccaccgcg agatcgagcc
     1861 gccggtcgcc atcgaaatcg gcggcacgca cgtccgtgac ccgcgcgatt ccgtcgacga
     1921 tcacgtgctt ggcgaaatgc gagtcgccct cgttttccag aatgatcacc gcgccgatct
     1981 tgtcgttgtt cggaaacacc tgccccatgc tcgcaatcag caggtccagg tcgccgtcgc
     2041 catcgatgtc aaccgcctcc gcccgcacgg gcgcggcgat atcgggcgcg agcacggttt
     2101 cgacgaaccg acccgacgcg gtctggcgca accacgaaac ggtgttcgcc ttcgcatcac
     2161 agacgacaat gtcgcagcgc ccatcatcgt cgaggtcgag cgcgttgacg tgagtgatcc
     2221 acggcttgcc gctcaccggc tccccaatgg gggtcgcgtg taaacggttc agctcttcag
     2281 cggtttcgac cgtctgccgc ggcggaattg cggccggcgc atccggccgg cggcaccccg
     2341 cccatcccgc tgcggccaga attccgcaca gcgcaaggcg gcacgggccg cccgataccg
     2401 gccggagccg cggaaaaaat ctgaggcgcg gggtcatgcg gcaagtcgac aggaaaagag
     2461 aagctgatcg aactctaaat gcaccccgct gcccgcgcgg accggacgat tgattgcgtg
     2521 gatttccgca tcatcatcga acgcggggaa cggggttgct taacggcctc gcgaaggctt
     2581 ttttcgccgc ttgcgcaaaa tttggaagag tcgccgcttc attcccgcgc tggcacttct
     2641 gtgcagcgtc agcggagtcg gacaggagca ccccggcgtc gaccggcaac cgttgccgac
     2701 tcgggcaaga cgcaccggcc ccgcgctgtt cacgacgctt ccgcccgaac agacaggaat
     2761 cgacgtgatc aaccgttacg atgacgtcag gatttggggc gatcgatacg aggaattcgc
     2821 cttcggctcg attggcaccg gggtcgcaat cgccgactac gatgcggacg gacgccctga
     2881 tgtgtttttg gcgggcaagt ccgcgggcgg ccgtctgtat cgaaatcttg gagactggaa
     2941 gttccgcgat gtcaccgaac catccgggct catcgcaccg gacgcgggct ggcaggcggg
     3001 cgcggcgttc gtcgatctcg ataatgacgg agatctggat ctttacatct gccgggccaa
     3061 cgagccgaac ttgctctatg tcaaccaagg cgacggcacc ttcaaagagg gcgccaaggc
     3121 cgccggcctc gatgtggtcg acgcgagcgt catggccgct ttcgccgact at
//
LOCUS       DASPLT010000598         1100 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_108743, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000598 DASPLT010000000
VERSION     DASPLT010000598.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1100)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1100)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1100
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_108743"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(3..500)
                     /locus_tag="VEA63_06240"
     CDS             complement(3..500)
                     /locus_tag="VEA63_06240"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF012413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0005542 - folic acid binding [Evidence
                     IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HYD83629.1"
                     /translation="MQALNLIVACAENRVIGRDGKLPWRIPEDWRFFKSRTAGATVIL
                     GRISFESWKSILDDDRHAIVLTRDASLARDRVQTARSLPEAIAHAQESSRPIYICGGQ
                     RIFEEAIAHPQATRLYLTLVHANVEGDRYFPEWRAHFPRVIEARPGADENFRYTFYTL
                     ARADA"
     gene            complement(500..>1100)
                     /locus_tag="VEA63_06245"
     CDS             complement(500..>1100)
                     /locus_tag="VEA63_06245"
                     /inference="COORDINATES: protein motif:HMM:NF012698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HYD83630.1"
                     /translation="PDLVVLDVDMPVLTGLEVCQELRRLHFESPILMLTVRSQVDDKV
                     AGLNAGADDYLSKPFEGRELIARVQALLRRWKREEQTRLTLEFGAVKVDLEKKTATRN
                     GEPLSLTKTEYALLELLAKHPDQPVSRETMLDIAWGYARFPNTRTVDTHIWRLRKKIG
                     DDGDEPRWIRRVHGQGYCLVTDPPASPAAPASPEREPGS"
BASE COUNT          181 a          309 c          401 g          209 t
ORIGIN      
        1 cgctacgcgt cggcgcgggc gagggtgtag aaggtgtagc ggaagttttc gtcggcgccg
       61 gggcgcgctt cgatgacgcg agggaagtgc gcgcgccatt cggggaaata gcggtcccct
      121 tccacgttag cgtggacaag cgtgagatac aggcgcgtgg cttgcggatg agcgatcgct
      181 tcttcgaaga ttcgctgtcc gccgcaaatg tagatggggc gagaggattc ttgcgcgtgc
      241 gcgatcgctt cgggcagcga gcgcgcggtt tgcacgcgat cgcgggcgag cgaggcatcg
      301 cgcgtgagca cgatcgcatg gcggtcgtcg tcgaggatac ttttccaaga ttcgaaactg
      361 atgcgaccga ggatcaccgt ggcgccggcg gtgcgggatt tgaagaagcg ccagtcctcc
      421 ggaatgcgcc acggcaattt gccgtcgcgg ccgatcacac ggttctcggc gcaggcgacg
      481 atgagattga gcgcttgcat cacgagccgg gctcgcgctc gggcgatgcc ggcgcggcgg
      541 gcgacgcggg cggatcggtg acgaggcagt agccctggcc gtgcacgcgg cgaatccagc
      601 gcggttcgtc gccatcgtcg ccgatttttt tccggagccg ccaaatgtgg gtgtcgaccg
      661 tgcgcgtgtt gggaaagcgt gcgtagcccc aggcgatatc gagcatggtc tctcgcgaca
      721 ccggttgatc gggatgtttc gcgaggagtt cgagcagggc gtattcggtc ttcgtgaggc
      781 tgagcggctc gccgttgcgc gtggcggttt tcttttcgag gtcgaccttc acggcgccga
      841 attcgagcgt gagccgggtt tgctcttcgc gtttccaacg gcgcaacagg gcttggacgc
      901 gcgcgatgag ctcgcgacct tcgaagggtt tcgaaagata atcgtcggcg ccggcgttga
      961 gccccgcgac cttgtcgtcc acttgcgagc gcacggtcaa catcaggatc ggcgactcga
     1021 aatgcagccg gcgcaattcc tggcagactt cgagaccggt gagcaccggc atgtcgacgt
     1081 cgagcacgac gaggtcgggc
//
LOCUS       DASPLT010000599         1485 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_212166, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000599 DASPLT010000000
VERSION     DASPLT010000599.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1485)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1485)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1485
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_212166"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1108
                     /gene="gndA"
                     /locus_tag="VEA63_06250"
     CDS             <1..1108
                     /gene="gndA"
                     /locus_tag="VEA63_06250"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007360564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HYD83631.1"
                     /translation="GELGARFGPSLMPGGDPSSWQHLKPIWDAIAAKVDEKTGKPLEN
                     YAPGKPVTGGVPCTAYIGPNGAGHYVKMIHNGIEYGDMQMICEAYALMSGLLGLKAEE
                     MGAIFSEWNEGALDSFLIEITADILKQKDPRNKRRAFVDIVLDTAGQKGTGKWTSTNA
                     LDMGVPAPTIAEAVFARCISAIKEERVAASKILKGPAKKYRGSKKALIQAIHDALYCS
                     KICSYAQGFQLMRTAQKEYNWKLNFGEIAQIWRGGCIIRAAFLQKITEAYARNPNLAN
                     LLLDPYFNKTVKQAQENWRKVVSLAAECGVAAPTFSSALAYYDSYRSARLPANLLQSQ
                     RDYFGAHTYERVDEKRGKFFHVDWPEASRPEIGA"
BASE COUNT          295 a          533 c          406 g          251 t
ORIGIN      
        1 cggcgagctc ggcgcacgct tcggaccctc cctcatgccc ggcggcgatc cctcctcctg
       61 gcaacacctc aagcccatct gggacgccat cgccgccaag gttgatgaaa aaaccggcaa
      121 accccttgag aactacgccc ccggcaagcc cgtcaccggc ggcgtcccct gcaccgccta
      181 catcggcccc aacggcgccg gccattacgt gaagatgatt cacaacggca tcgagtatgg
      241 cgacatgcag atgatctgtg aggcctacgc cctgatgtct ggtctcctcg ggctcaaagc
      301 cgaagaaatg ggcgccatct tctccgagtg gaacgaaggc gcgctcgaca gtttcctcat
      361 cgaaatcacc gccgacatcc tcaagcagaa ggacccgcgc aacaaacgtc gcgccttcgt
      421 cgacatcgtc ctcgacaccg ccggtcagaa aggcaccggc aagtggacgt cgaccaacgc
      481 cctcgacatg ggcgtccccg cgccgacgat cgccgaagcc gtcttcgccc gctgcatctc
      541 cgccatcaag gaagagcgcg tcgccgcttc gaagattctc aaaggccccg cgaagaaata
      601 ccgcggctcg aagaaagcac tcatccaggc gatccacgac gcgctgtatt gctcgaagat
      661 ctgctcgtat gcgcagggct tccaactgat gcgcaccgcg caaaaggaat acaactggaa
      721 gctcaacttc ggcgagatcg cgcagatctg gcgcggcggc tgcatcattc gcgccgcgtt
      781 cctccagaag atcaccgagg cctacgcgcg gaatccgaat ctcgcgaatc tcctcctcga
      841 tccgtatttc aacaagacgg tgaagcaggc gcaggaaaac tggcgcaagg tcgtctcgct
      901 cgccgccgag tgcggcgtcg ctgcgccgac gttctcctcc gcgctcgctt actacgacag
      961 ctatcgctcc gcccgcctgc ccgcgaacct gctgcaatcg cagcgcgatt acttcggcgc
     1021 acacacctac gagcgcgtcg acgaaaagcg cggcaagttc ttccacgtcg attggcccga
     1081 agcctcccgc cccgagatcg gcgcgtaagt caaccaacga caacgtagcg gcaggcctcc
     1141 gtgcctgccc gctttcgttt tcaaggtagg tggcgcgctc gccgcgccac gccctctccc
     1201 gaggacaagc ttcggcgctg cgcctgcagc tttcgcgcac gcgcgcattt cagcaggcag
     1261 gcacggaggc ctgtcgctac gcgaatgcgt ttagcgaccg ccccgttgtc cccttgcaag
     1321 attgcagtcg cccgacccac gcgaacattg tccgcctcat gggctcagtc ctgcacgtcc
     1381 tttacgccga ggacaatacg gccgatgccg aattgacgcg cgctcatttc gagcgattcg
     1441 cgccggactt cgcgctcgag atcgtgccac gcgccgatgc gtttc
//
LOCUS       DASPLT010000600         2420 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_253133, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000600 DASPLT010000000
VERSION     DASPLT010000600.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 2420)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2420)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2420
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_253133"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1249
                     /locus_tag="VEA63_06255"
     CDS             <1..1249
                     /locus_tag="VEA63_06255"
                     /inference="COORDINATES: protein motif:HMM:TIGR01891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HYD83632.1"
                     /translation="AAQIAADYPSLESLYRHFHANPELSLMEEKTAAKLAAELRAAGC
                     EVTENFGGTGVVAVMKNGEGPTLLLRADLDALPVREETGLPYASTVRVNDLSGQEVPV
                     MHACGHDVHLTVLVGAARRLAALKDRWSGTLVLIGQPAEERGLGARAMLSAGLYRLYP
                     KPDYALALHDSASLPAGTVGTLEGFIMANVETLEITVRGQGGHGAAPHLTKDPVVLAA
                     RIVLALQTIVSRELRPGEPAVITVGSIHGGTKSNIIPDEVKLQLTLRSYSDEVRRYLV
                     DAIARICRGEALAAGLPEDRLPILTLAEGDFTPSLYNDPALTQRVTGVLADWLGATNL
                     KPVQPEMIGEDFSRYGRTTEKVPSTMIRLGAVPQKTWDEAQRTGAPLPSLHSSKFAPD
                     PEPTIKTGVTVLTATALDLLAKP"
     gene            1553..2092
                     /locus_tag="VEA63_06260"
     CDS             1553..2092
                     /locus_tag="VEA63_06260"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HYD83633.1"
                     /translation="MRSFVYLALLGVILITIAFVVRSGLLARKDPGRPINTAMREALD
                     KDQQGTPELSSADADIVRHKYPTATRTDSGLIYLVRAPGTGQPPRFGDEVIVHYDGRL
                     LDGTPFDSSYRSGAPITFRVGTGAVIKGWDEALLTMRKGEKRTLIIPHWLAYGAAGRP
                     PNIPPRATLVFEVELIDFR"
BASE COUNT          422 a          929 c          636 g          433 t
ORIGIN      
        1 cgccgcccaa atcgccgccg attacccgtc gctcgaatcg ctctaccgcc atttccacgc
       61 gaaccccgag ctctcgctca tggaggaaaa aaccgccgcg aaactcgccg ccgaactccg
      121 cgccgccggt tgcgaagtca cggaaaattt cggcggcacc ggcgtcgttg ccgtcatgaa
      181 aaacggcgaa ggccccacgc tcctgctccg cgccgatctc gatgccctcc ccgttcgcga
      241 ggaaaccggc ctcccttacg cgagcaccgt ccgcgtgaac gacctctccg gccaggaggt
      301 ccccgtcatg cacgcctgcg gccacgatgt tcacctcacc gtcctcgtcg gcgccgcccg
      361 ccggctcgcc gcactaaaag atcgctggtc gggcaccctc gtcctcatcg gccagcccgc
      421 cgaggaacgc ggcctcggcg cgcgcgccat gctctcggcc gggctctatc ggctgtatcc
      481 gaaacccgat tacgccctcg cgctgcacga cagcgcctcg ctgcccgccg gcaccgtcgg
      541 caccctcgag ggcttcatta tggccaacgt cgaaacgctc gagatcaccg tgcgcggcca
      601 aggcggccac ggcgcggcgc cgcatctcac gaaagatccc gttgtcctcg ccgcccgcat
      661 cgtgctcgcc ctgcagacga tcgtcagccg cgaactccgc cccggcgaac cggcggtgat
      721 caccgtcggc tccatccacg gcggcacgaa gagcaacatc atccccgacg aggtgaaact
      781 tcagctcacg ctgcgttcct actccgacga agttcgccgc tatctggtcg acgccatcgc
      841 gcgcatctgt cgcggcgagg cgctcgccgc cggtttacct gaagaccgtc tgccgatcct
      901 cacgcttgcc gaaggcgatt tcacaccgtc gctctacaac gaccccgcgc tcacacaacg
      961 cgtcaccgga gtgctcgcgg attggctcgg cgccacgaac ctcaaacccg ttcaacccga
     1021 aatgatcggc gaagacttca gccgctacgg ccgcaccacc gaaaaagtcc cgagcacgat
     1081 gatccgcctc ggcgccgtcc cgcaaaaaac ctgggacgaa gcccagcgca ccggcgcgcc
     1141 ccttccttca ctccactcga gtaaattcgc tcccgatccc gagcccacca tcaagaccgg
     1201 cgtcaccgtc ctcaccgcca ccgcactcga tctcctcgcg aagccctgac cagatatcat
     1261 gcggatcccg ccggcgcccc accgacccca acgcacgcca actccgcagt gttcagtttc
     1321 tatttcggcg atcatccttc ttcctccccg gcgtctttaa cgcccatggt cggaccggtt
     1381 ttctttcgtg tccattcgtg gttcaaaccc gttcgaaaat ttcggcctga atccgtgttc
     1441 atcagtgtcc atccgtggtt gaactaaacg gttcgaccaa actcaacgcg cccacgcagc
     1501 ctctgggttt gcaaattcct caatcctccc cgaaatccgc ttcgcttttc ccatgcgcag
     1561 cttcgtctat ctcgccctgc tcggtgtcat cctcatcacg atcgcgttcg tcgtccgctc
     1621 cggcctgctc gcccgcaagg accctggccg cccgatcaac accgcgatgc gcgaagcgct
     1681 cgacaaggac caacaaggca cccccgagct cagcagcgcc gacgctgaca ttgtccgcca
     1741 taaatacccc accgccacgc gcaccgactc cggtctcatt tatctcgttc gcgcccccgg
     1801 caccggacaa ccgccgcgtt tcggcgacga agtcatcgtg cactacgacg gccgtctcct
     1861 tgatggcacg cccttcgaca gctcgtatcg cagcggcgct ccgatcactt tccgcgtcgg
     1921 caccggcgcc gtgatcaagg gctgggacga agcgcttctc accatgcgca aaggcgagaa
     1981 acgcacgctc atcattcctc actggctcgc ctacggcgcc gcgggccgcc cgcccaacat
     2041 cccgccccgc gccacgctcg tcttcgaagt ggagctgatc gattttcgct gagcacgcgg
     2101 tcgatcgcga accgacgtcc ggctccaatt ttttcctccc gagccatgcg cgtcgctcac
     2161 cagttcgctc ggctctttgt tttcgccgcg accgctcttt cgccgtgcac ggcaaacgcg
     2221 gctgatgtcg cgagcgttct ccagggagcg cgcgcgctcg ttcagcggga ggtcgcttcg
     2281 aaggtgccgg gggcctcggt cgctgtcgca gtggatggcg ttctggtgtg gcaggagaat
     2341 ttcggcgtag ccgacgtgaa ggccgcgaga ccggtcacgg cgcacacgcg atttcgcatc
     2401 ggcagtattt caaaatcgct
//
LOCUS       DASPLT010000601         1139 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_150468, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000601 DASPLT010000000
VERSION     DASPLT010000601.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1139)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1139)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1139
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_150468"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            27..941
                     /locus_tag="VEA63_06265"
     CDS             27..941
                     /locus_tag="VEA63_06265"
                     /inference="COORDINATES: protein motif:HMM:NF037959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HYD83634.1"
                     /translation="MKALCVLAALLAPFAMSKAAAAADGDYIEHFDTIYNSLTVEKRG
                     SVVELRAHARGSEALESAVDLNNPLNLLVPYTRTLYAAMFFQPEPKRVLMIGLGGAGF
                     HRPFAAAFPEALLQTAELDPKVLELCETRLGWKPTAQTPVELMDGRMFVKRNREKWDW
                     LILDAFRGGFVPPHLKTEEFYRECAARLDDRGVFVSNLHPNSQLYYSDIKTIREVFPQ
                     VIFFQTQGRGNVIAVAVKYRTPSIADDAKWPASDELTRRLRGLVDMERVKQGRARIPE
                     AQVAKADLLTDDFAPVEFLNAIEAGNVN"
BASE COUNT          211 a          332 c          377 g          219 t
ORIGIN      
        1 ctccgcaacc ccgattctgc gtccgcatga aagctctctg tgttctcgcc gcgctgctcg
       61 ctccattcgc gatgtcgaaa gccgccgccg cggccgacgg cgactatatc gaacatttcg
      121 atacgatcta caacagcctg accgtggaga aacgcggttc ggtggtggag cttcgggcgc
      181 acgcccgagg cagcgaagcg ctcgaatcgg cggtggattt gaacaacccg ctgaacctgc
      241 tcgtgcctta cacgcgcacg ctctacgcgg cgatgttctt ccagccggag ccgaagcgcg
      301 tgctgatgat cggcctcggc ggcgcgggat ttcaccggcc gttcgcggcg gcgtttcccg
      361 aagcgttgct tcagaccgcg gagcttgatc cgaaggtgct cgagttgtgc gaaacgcggt
      421 tgggttggaa gccgacggcg cagacgccgg tggagttgat ggacggccgg atgttcgtga
      481 agcgcaaccg cgagaaatgg gactggctga ttctcgatgc gtttcgcggc gggtttgtgc
      541 ctccgcattt gaaaaccgag gagttttatc gcgagtgcgc ggcgcggttg gacgatcgcg
      601 gcgtgttcgt gtcgaatctc catccgaaca gccagctcta ctactccgac atcaagacga
      661 tccgcgaagt gttcccgcag gtgatctttt tccaaacgca aggccgcggg aacgtgatcg
      721 cggtcgcggt gaagtatcgc acgccgtcga tcgctgacga tgccaagtgg ccggcgtcgg
      781 acgaactcac gcgacgcctg cgcgggctgg tcgacatgga acgcgtgaaa caagggcgcg
      841 cgcgaattcc cgaggcgcag gtcgcgaagg cggatttatt gacggacgat ttcgcgcccg
      901 tggagtttct caacgcgatc gaagcgggga acgtgaattg agccgtgcgg gcgggtaacc
      961 gcgggggttg acctgcggac cgcatcgcaa ccgatgcggc tacgggcgag gtcgagtttt
     1021 agaccgcgaa ttccctcgcg gagttgcggg tgaacggaga aggctgagac tctacttcac
     1081 ctcatgagcc tcttcatcgg catcgattcc ggcacccaaa gcgtcaaggc agtcgtcct
//
LOCUS       DASPLT010000602         1046 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_294372, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000602 DASPLT010000000
VERSION     DASPLT010000602.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1046)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1046)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1046
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_294372"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..781
                     /locus_tag="VEA63_06270"
     CDS             <1..781
                     /locus_tag="VEA63_06270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007360323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HYD83635.1"
                     /translation="GIPIRARNLEQQFFLDALLDDSVSIVTCFGKAGTGKTLLSIAGA
                     LHQTQDENSRYDGLSISRPVIALGKDIGFLPGTLEEKMKPWLQPYYDALEVLMPSKPP
                     KEPQFASKKVSKKKHKKSDERYLAALAAPAPSQASHNGGHGNAAVAKPYERLLRTGLV
                     EIEALAFIRGRSIARRFFILDEAQQLTPHEAKTVITRISEGSKIVLIGDPAQIDNPYV
                     DARSNGLVYCHNRMKGQSLAAHVKLTKGERSRLAELAADLL"
BASE COUNT          205 a          300 c          361 g          180 t
ORIGIN      
        1 aggaatcccg attcgcgcgc gcaatctgga gcagcagttt ttcctggatg cgttgctcga
       61 cgacagtgtg agcatcgtca cgtgtttcgg caaagccggc acgggcaaga cgctgctgtc
      121 gatcgcgggc gcgctgcacc agacgcagga cgagaattcg cgctacgacg ggctgtcgat
      181 ctcgcggccg gtgatcgcgc tcggcaagga catcgggttt ctaccgggca cgctcgagga
      241 gaagatgaag ccctggctgc agccctacta cgacgcgttg gaagtgttaa tgccctcgaa
      301 gccgccgaag gagccgcagt tcgcgtcgaa gaaagtttcc aagaagaagc acaagaagag
      361 cgacgagcgt tatctcgccg ctttggcggc gcccgctccg agtcaggcga gtcacaatgg
      421 cggacacggc aacgccgcgg tggcgaagcc ctacgagcgg cttttgcgca cggggctcgt
      481 ggagatcgaa gcgctcgcgt tcattcgcgg acgctcgatc gcgcggcggt ttttcatctt
      541 ggacgaagcg caacagctca cgccgcatga agcgaagacg gtgatcacac gcatctcgga
      601 aggctcgaag atcgtgctga tcggcgatcc ggcgcagatc gacaatcctt atgtggacgc
      661 gcgcagcaac ggcctcgttt actgccacaa tcgcatgaag ggccagtcgc tcgcggcgca
      721 cgtgaaattg acgaagggcg agcgttcgag actcgcggaa ctcgcggcgg atttgttgta
      781 ggcgacgcag cgcgcgaggc gaagcgcgcg gtgatgtagc ggcaggcttc cgtgcctgcc
      841 tgctcgattg ttgtggggtt gcgcgctggc gcgcaaccgt ggtgcggcga gcgcaccacc
      901 tacaatgccg aagtggtgcg gcgagcgcac cacctacatt cgaacagagc agcaggcagg
      961 cgcggaggcc tgccgctaca gcatgcggat cggcttcagg agatttttcg agcggcttgt
     1021 tgggcgcgga ggcagagttc ggcggc
//
LOCUS       DASPLT010000603         1023 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_192229, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000603 DASPLT010000000
VERSION     DASPLT010000603.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1023)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1023)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1023
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_192229"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1023
                     /locus_tag="VEA63_06275"
     CDS             <1..>1023
                     /locus_tag="VEA63_06275"
                     /inference="COORDINATES: protein motif:HMM:NF013400.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="beta-xylosidase"
                     /protein_id="HYD83636.1"
                     /translation="VHHLHTSGDGTLALKWSSTNVYTEDAEGNPVYDWKLIDEIMDEL
                     TRPGLEPFVEVSFMPRALSAKPDPYTPTLTKRGLPGNDILSGGAYHPPKDYQKWQAFM
                     ETWVRHCVERYGEAAVSSWLWELWNEPESPYFKGTVEDYCRLYDHFAAAVKKVVPNAR
                     VGAPHTTDPKWRKGDEFMVAFLEHCRSGRNAVTGAVGAPLDFIAYHAKGLTRLDEQRR
                     VEMDLRNHLWSIDLYSGIIARFPEFAQLPVYIGESDPEGCAGCPSTLDPQRDYRRTSQ
                     FAAYTAASFMRKQDLVSSHGGNLQGAVSWAFTFHDQPWFNGLRALTTNEVALPVFNAF
                     KLFARLG"
BASE COUNT          197 a          312 c          317 g          197 t
ORIGIN      
        1 cgtgcatcac ttgcacacga gcggcgacgg cacgctggcg ctgaagtggt cgagcacgaa
       61 cgtctacacc gaagatgctg agggaaatcc ggtctacgat tggaagctga tcgacgagat
      121 catggacgaa ctcacgcgtc ccggcctcga gccgtttgtt gaagtgagtt tcatgccgcg
      181 cgctctctca gcaaaacccg atccttacac tccgacgctt acgaaacgcg gcctgccggg
      241 gaacgacatc ctttccggtg gcgcctacca tccgccgaag gattaccaaa agtggcaggc
      301 gttcatggaa acgtgggtgc gccattgcgt ggagcgttac ggtgaagcgg cggtttcctc
      361 ttggttgtgg gaactgtgga acgaaccgga gtcgccctac ttcaagggca cggtcgaaga
      421 ctactgccgg ctctacgatc attttgccgc cgcggtaaag aaagtggtgc ccaacgcgcg
      481 agtgggtgcg ccgcacacga cggatccgaa gtggaggaaa ggcgacgagt tcatggtcgc
      541 gtttctcgag cactgccgtt cggggcgaaa cgcagtcacg ggcgcggtcg gcgcgccgct
      601 cgatttcatc gcgtatcacg cgaaaggcct tacgcggctc gacgagcagc ggcgggtgga
      661 gatggacctg cggaatcacc tctggtcgat cgatctctac agcggcatca tcgcccgctt
      721 cccggagttc gcccaattgc cggtctatat cggtgagtcg gatccggaag gttgcgccgg
      781 ctgcccttcg acgctggatc cgcagcgcga ctatcgccgc acctcgcaat tcgccgctta
      841 cacggctgcg tcgttcatgc gcaaacagga cctcgtcagc agccacggcg gcaatctgca
      901 gggcgccgtc tcgtgggcgt tcacgtttca cgatcaaccg tggtttaacg gactgcgcgc
      961 actcaccacg aacgaggtcg cgttgccggt gttcaacgcc ttcaaacttt tcgcccgcct
     1021 cgg
//
LOCUS       DASPLT010000604         2581 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_192233, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000604 DASPLT010000000
VERSION     DASPLT010000604.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 2581)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2581)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2581
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_192233"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..577)
                     /locus_tag="VEA63_06280"
     CDS             complement(<1..577)
                     /locus_tag="VEA63_06280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD83637.1"
                     /translation="MKKQARWLAIFVLLAATAAAAPFIVVVYNVENLFDADGKAGFED
                     YQPTRYSRGHVLTKLQNISRLLARFENGRGPDVILFQEIEADQTPGATAPRYDEILRR
                     YVDVRIEEMLGAKFDDAVADLPAEALLAKAMADQGMTGYHVVAAENVTADGSTRALAQ
                     ACVVFTRFPVKAVRSHPTLDARAVLEVQVEVE"
     gene            complement(591..1106)
                     /locus_tag="VEA63_06285"
     CDS             complement(591..1106)
                     /locus_tag="VEA63_06285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD83638.1"
                     /translation="MKVPPLLPEETFHRVMRVANRDGLSVMAVAGMLALAAASIRDFS
                     GAAVGLAVAAAGAMELHGAGLLRAGERRGMKWILVSQPYLMAVLLAYCAVRLATFDAA
                     VLQEAVTDDLRQKIAEAGFTEERMLRQAYTLMYGLLAIGTVIYQGAMTLYYWRRRSAV
                     EAAAVDDNRFA"
     gene            1226..>2581
                     /locus_tag="VEA63_06290"
     CDS             1226..>2581
                     /locus_tag="VEA63_06290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012372839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HYD83639.1"
                     /translation="MSARPAKAFPFRLEFPPELPISARAEEITAAIAAHPVVILAGET
                     GSGKTTQIPKMCLAAGRGTDGRIACTQPRRVAALSVSRRVADELNVAWGREVGCKIRF
                     NDETTRDTVVKFLTDGMLLAEVQGDPLLREYDTIIIDEAHERSLNIDFLLGHLRTLRY
                     KRPELKIVITSATIDTEAFSQAFDGAPIIQVEGRTYPVEVIYAPLDELGSDAAEGDER
                     ESKAEALHYIDGAVEAVDRILHESNAGDALVFLPSERDIREVRDLLEGRRLRDCELVP
                     LFGRLSNAEQQRVFAPTQRRKIILATNIAETSLTIPGIRFVVDTGLARISRYSPQART
                     RRLPIEPVSQSSADQRKGRSGRVADGVCIRLYSETDYLERPRFTQPEIQRANLADVIL
                     RMKAFGLGDIERFPFINMPAAKSIRAGYALLEELGALETCRAGPPDPAAPTAPDADRR
                     GQ"
BASE COUNT          478 a         1019 c          660 g          424 t
ORIGIN      
        1 cttcgacctc cacttgcacc tcgagcaccg cgcgcgcatc gagcgtcgga tgcgaacgca
       61 ccgccttcac cggaaaccgc gtgaagacca cgcaggcctg cgcgagcgcc cgcgtgcttc
      121 cgtccgccgt gacattctcc gccgccacca cgtgataacc cgtcatcccc tgatctgcca
      181 tcgccttcgc cagcagtgcc tccgccggca gatccgcgac cgcgtcgtcg aacttcgcgc
      241 cgagcatctc ctcgatccgc acatccacat aacgccgcaa aatttcgtca taacgcggcg
      301 ccgtcgcacc cggcgtttgg tccgcttcaa tttcttgaaa cagaatcacg tcgggcccgc
      361 gcccgttctc aaaccgcgcg agcaaccgtg aaatattctg cagcttcgtc agcacgtgcc
      421 cccgcgaata tcgcgtcggt tgataatcct cgaaccccgc cttgccatcc gcgtcgaaga
      481 gattctcgac gttgtaaacc accacgatga acggcgccgc cgccgcggtc gccgccagca
      541 gcacgaagat cgccagccat cgcgcttgtt ttttcatcgc ggcatacggc tcaggcgaac
      601 cggttgtcat caaccgccgc cgcttcgacc gccgaccgcc gccgccagta gtaaagcgtc
      661 atcgcgcctt ggtaaatgac cgtcccaatc gcgagcaaac catacatcaa cgtatacgcc
      721 tgccgcagca tgcgctcttc ggtaaacccc gcctcggcga ttttttgccg cagatcgtcc
      781 gttaccgctt cttgcagcac cgccgcgtcg aacgtcgcga gccgcaccgc gcaatacgcc
      841 agcaacaccg ccatcaaata cggctggctc acgagaatcc atttcatccc ccgccgctcg
      901 ccggcccgca acaatcccgc gccgtgcaac tccatcgcgc ccgccgccgc caccgccagg
      961 cccaccgccg ccccgctgaa atcgcggatc gatgccgccg cgagcgccag catgccagca
     1021 accgccatca cgctcaaccc gtccctattt gccacgcgca tcacgcgatg gaacgtttcc
     1081 tccggcaaca acggcggcac cttcatcgcg aatggagccc cggcgcccgc gccgcaccgc
     1141 cttcgatcgc aaaacaattc tgttggatgt cgctcacgag ccgttctgga ttcctccctt
     1201 ccctggtaat gacaacctaa atttcgtgtc cgcccgacct gccaaagcct ttcctttccg
     1261 cctcgagttt ccgcccgagc tgccgatcag cgcgcgcgcc gaagagatca ccgcggccat
     1321 cgccgcgcat cccgtcgtca tcctcgccgg cgaaaccggc tccggcaaaa ccacacagat
     1381 ccccaagatg tgcctcgctg ccggtcgcgg cacggacggc cgcatcgcgt gcacgcagcc
     1441 ccgccgcgtc gccgcgctct ccgtctcccg ccgcgtcgcc gacgaactca acgtcgcctg
     1501 gggccgcgag gtcggctgca agattcgttt caacgacgaa accacccgcg acaccgtcgt
     1561 taaatttctc accgacggca tgctgctcgc cgaagtccag ggcgacccgc tcctccgcga
     1621 atacgacacg atcatcatcg acgaagcgca cgagcgctcg ctcaacatcg acttcctcct
     1681 cggccatctg cgcacgctgc gctacaaacg tcccgagctc aaaatcgtca tcacgtccgc
     1741 cacgatcgac accgaggcgt tcagccaggc cttcgacggc gcgccgatca tccaggtcga
     1801 aggccgcacg tatccggtgg aagtgattta cgctcccctc gacgaactcg gcagcgacgc
     1861 cgccgagggc gacgaacgcg aatccaaagc cgaggcgctt cactacatcg acggcgccgt
     1921 cgaagccgtc gatcgcatcc tccacgagag caacgccggc gacgcgctcg ttttcctccc
     1981 cagcgagcgc gacatccgcg aggtccgcga cttgctcgag ggccgtcgcc tccgcgattg
     2041 cgagctcgtc ccgctcttcg gccggctttc caacgccgag caacaacgcg tcttcgcccc
     2101 cacgcagcgc cgcaaaatca ttctcgcgac caacatcgcc gagacctcgc tcacgatccc
     2161 cggcatccgc ttcgtcgtcg acaccggcct cgcgcgcatc agccgctatt cgccgcaggc
     2221 ccgcacgcgt cgcctaccga tcgagcccgt ttcgcaatcc agcgccgacc agcgcaaagg
     2281 ccgcagcggc cgcgtcgccg acggcgtttg cattcggctg tattcggaaa ccgactactt
     2341 ggaacgtccg cgcttcacgc agccggagat tcaacgcgcc aacctcgccg acgtgatcct
     2401 gcgcatgaaa gcgttcggcc tcggcgacat cgaacgtttc cccttcatca acatgcccgc
     2461 cgcgaaatcc attcgcgccg gctacgccct cctcgaagaa ctcggcgccc tggaaacatg
     2521 tagggcgggg cctcccgacc ccgccgctcc gactgctccc gacgccgatc ggcggggtca
     2581 g
//
LOCUS       DASPLT010000605         1569 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_150513, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000605 DASPLT010000000
VERSION     DASPLT010000605.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1569)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1569)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1569
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_150513"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..456)
                     /locus_tag="VEA63_06295"
     CDS             complement(<1..456)
                     /locus_tag="VEA63_06295"
                     /inference="COORDINATES: protein motif:HMM:NF012312.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HYD83640.1"
                     /translation="MSLPTAGVEPIRNSPRRVLLASLAGTTIEFFDFYIYATAAVLVF
                     PKLFFPASDPASATLQSLATFALAFFARPVGSAVFGHFGDRIGRKATLVAALMTMGVS
                     TVVIGFLPTYASIGMAAPVLLAVCRFGQGFGLGGEWGGAVLLATENAPPG"
     gene            776..>1569
                     /locus_tag="VEA63_06300"
     CDS             776..>1569
                     /locus_tag="VEA63_06300"
                     /inference="COORDINATES: protein motif:HMM:NF024869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HYD83641.1"
                     /translation="MHPPFPRLTHRAMVATPTFYAQLAALESVGAPFVLVVLAEAFGS
                     TPQDAGAKMLVTPAGLLAGTVGGGRVEAKAIALAQELLAATGADAAKPRFVTWTLKTD
                     VGMTCGGSVKLYFEPHAAGGAAWPIAIFGAGHITQALLPVLLPLTCTIAVYDTRAEWL
                     AKLPRAANLRAERVDDLAAIVDTLPADASVLSMTQGHATDRPILFAHSPRETFLTSAA
                     SEAMRKPPSSAANSSPTVSRSTAPPTSTAPSASPSARTIRTKSPSAS"
BASE COUNT          286 a          653 c          383 g          247 t
ORIGIN      
        1 acccggcggc gcgttctccg tcgccagcaa caccgctccg ccccactcgc cccccagccc
       61 gaaaccctgc ccaaaacgac acactgccag cagcaccggc gccgccatgc cgatcgacgc
      121 atacgtcggc agaaagccaa tcacgaccgt cgaaacaccc atcgtcatca acgccgcgac
      181 caacgtcgcc ttgcgcccga tccgatcgcc gaaatgtccg aacaccgccg accccaccgg
      241 ccgcgcgaaa aacgccagcg cgaaggtcgc gagcgattgc aatgtcgccg acgccggatc
      301 cgacgcgggg aaaaacaact tcggaaacac cagcaccgcc gccgtcgcat aaatatagaa
      361 atcgaaaaac tcgatcgtcg tgcccgcgag gctagcgagc agcacgcgac gcggggaatt
      421 tctaatcggc tcaacaccgg cggtagggag gctcatagcg ggggcgaagt aaaatgtgcg
      481 aaacagtcac agcgcccgcg tatcgaaatt acaacgacaa cccgcacgcc gccaaacgca
      541 tccgattatg cgctccggct tctcgcgtca atgttcgccc caaacgcact gcgcgtccac
      601 cctgcgcaaa attgctgtaa ggcggggcct ctcccgcccc gccgtctccc tccccccctc
      661 cataccctga tcccccactc gttctcgttc tctttctctt tctcgttctc ctccgccccc
      721 gcttccgtcc cctcgatctc cacgcttcgc cgcccgcccg caccgttttc tccctgtgca
      781 tccgcccttc ccgcggttaa ctcatcgcgc gatggtcgcc acgcccacct tctacgctca
      841 actcgccgcg ctcgagtccg tcggcgcccc cttcgtcctc gtcgtcctcg ccgaagcgtt
      901 cggcagcacg ccgcaggacg ccggcgcgaa aatgctcgtc acgcccgccg gtctcctcgc
      961 cggcactgtc ggcggcgggc gcgtcgaagc caaagccatc gccctagctc aggagcttct
     1021 cgccgccacc ggcgccgacg ccgcgaaacc acgcttcgtt acttggacgc ttaaaaccga
     1081 cgtcggcatg acctgcggcg gctccgtcaa actctacttc gaaccccacg ccgccggcgg
     1141 cgccgcctgg cccatcgcca ttttcggtgc cggccacatc acgcaggcac tgctgcccgt
     1201 tctcctcccg ctcacctgca ccatcgcggt ctacgacacg cgcgcggagt ggctcgcgaa
     1261 actaccgcgc gccgcaaacc tccgcgccga acgcgtcgac gacctcgccg cgatcgtcga
     1321 cacgttgccc gccgacgcct ccgtcctcag catgacgcaa ggccacgcca ccgaccgccc
     1381 gattcttttc gcgcactcgc cacgcgaaac tttccttacc tcggctgcat cggaagcgat
     1441 gcgaaagcca ccgtcctccg ccgcgaactc gtcgccgacg gtctctcgct cgaccgcgcc
     1501 gcccacttcc actgccccat cggcctcccc ttcggcacga accatccgca cgaaatcgcc
     1561 ctcagcatc
//
LOCUS       DASPLT010000606         1928 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_89890, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000606 DASPLT010000000
VERSION     DASPLT010000606.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1928)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1928)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1928
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_89890"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            509..1927
                     /gene="uxaC"
                     /locus_tag="VEA63_06305"
     CDS             509..1927
                     /gene="uxaC"
                     /locus_tag="VEA63_06305"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012377092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HYD83642.1"
                     /translation="MHSFIHEDFLLETDAARDLYHHFAKDEPIYDYHCHLPPQQIVDN
                     HQFADLAEIWLGGDHYKWRAMRSNGVDERFCTGNASPREKFDAWVATVPHTLRNPLYH
                     WSHLELARYFGISDLINPSSADKIWREANAKIPSLRVHDILAANKVAVICTTDDPADP
                     LDLHAKIRQLDLKTRVYPTFRPDKALVVDAPAAFNAWLEKLAGVTKTPIRSFDDFLAA
                     LKQRHADFHAIGGRLSDHGMENCYAEACTPAEAKTIFDAARAGRAATAADHAKFASFM
                     MLEFGRWDARAGWTKQLHLGAQRSNNTRLLAKLGPDTGFDSIGDYPQTRALSRYLDTL
                     DSTDELPRTILYNLNPADNYTFGTMIGNFQDGSIPGKLQLGSGWWFLDQKEAMEWQMN
                     ALSNLGLLSRFVGMLTDSRSFLSYPRHEYFRRILCNLLGRDMERGEIPNDRQLVGDLA
                     RRISFANARDYFRLELDPAFAK"
BASE COUNT          401 a          724 c          455 g          348 t
ORIGIN      
        1 cgctcgaaga aatgctcaac gacatctcgc gctgaccggc gcttcggaat gaaccgcgga
       61 gacacaaagc cacagagaaa atcggcgatc ggatgcctcc gtcgttcaag ggattggtcc
      121 ggatcgaaat cgtcagcccg atccggctca tccgcgtcca tcccgtgagt agaggcctcg
      181 ctccgacatt cgcgtccctg accggatctc tgcagaagac ctcccgcgaa tggcgcgaat
      241 ggacgcgaat ctcagggacg aaatcaaatc cagccgcttc aaaaccaggt cactgtccgg
      301 cgaaaaatca tttctcttca ttcatattcg ccgtaattgg cgtgattcgc gggcaactga
      361 attgatcccg gcttagcatc cattcacggt tcaaaaatca gcaccgcccg gaacttctcc
      421 gcctctgatc cgtgttcatc ggtgtccatc cgtggttaac cggccaacct ccgcattccg
      481 atctcccatc tccgaacttc gatctccgat gcattccttc attcacgaag acttccttct
      541 cgaaactgac gccgcgcgcg atctctacca ccatttcgcc aaggacgagc cgatctacga
      601 ttaccactgt catctcccgc cgcagcagat cgtcgacaac caccagttcg ccgacctcgc
      661 cgaaatctgg ctcggcggcg atcactacaa atggcgcgcc atgcgctcca acggcgtcga
      721 cgaacgcttc tgcaccggca acgcctcgcc ccgcgagaaa ttcgacgcct gggtcgccac
      781 cgttccccac acgcttcgca atccgctcta tcactggtcg cacctcgagc tcgcccgcta
      841 cttcggcatc tccgacctca tcaacccgtc ctccgccgat aaaatctggc gcgaagccaa
      901 cgcaaaaatc ccgtcgctcc gtgttcacga tatcctcgcc gccaacaagg tcgctgtcat
      961 ctgcacgacc gacgatcccg ccgacccgct cgatctccac gcgaaaatca gacagctcga
     1021 cctgaagact cgcgtttatc cgaccttccg ccccgacaag gcgctcgtcg tcgacgcccc
     1081 cgccgccttc aacgcctggc tcgagaaact cgccggcgtc accaaaactc cgatacgctc
     1141 cttcgacgac ttcctcgccg cactgaaaca gcgccacgcc gacttccacg ccatcggcgg
     1201 ccgcctctcc gaccacggca tggaaaactg ctacgctgaa gcgtgcacgc ccgccgaagc
     1261 caaaacgatc ttcgacgccg cccgcgcggg acgcgccgcc accgccgccg accacgcgaa
     1321 gttcgcgagc ttcatgatgc tcgagttcgg ccgctgggac gcccgtgccg gctggacgaa
     1381 acagctccac ctcggcgcgc agcgtagcaa caacacccgt cttctcgcga agctcggccc
     1441 cgacaccggc ttcgattcga tcggcgatta tccgcaaacc cgcgctctct cccgctatct
     1501 cgacaccctc gactccaccg acgagctccc gcgcacgatt ctctacaact tgaatccagc
     1561 cgacaactac accttcggca cgatgatcgg caatttccag gacggcagca ttcccggcaa
     1621 actccagctc ggcagcggct ggtggttcct cgaccagaaa gaagcgatgg agtggcagat
     1681 gaacgcgctg tcgaatctcg gcctcttgag ccgcttcgtc ggcatgctca cagactcacg
     1741 cagcttcctg agttatccgc gccacgaata cttccgccgc atcctctgca atctcctcgg
     1801 tcgcgacatg gagcgcggcg aaatcccgaa cgaccgccaa ctcgtcggcg acctcgcccg
     1861 ccgcatcagc ttcgccaacg cccgcgacta cttccgcctc gagctcgacc ccgccttcgc
     1921 aaagtagc
//
LOCUS       DASPLT010000607         3347 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_253242, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000607 DASPLT010000000
VERSION     DASPLT010000607.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 3347)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3347)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3347
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_253242"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..611)
                     /locus_tag="VEA63_06310"
     CDS             complement(<1..611)
                     /locus_tag="VEA63_06310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD83643.1"
                     /translation="MMGILACSPLIDAATMLAPGIAPPGAKVEVDRRIVLTIGPLELS
                     AYAVEKNVNRFRGTGGRRPARSVEEVRDWFRLFLAQQVVIAKAQEEGYLERPEVMAMV
                     ERMAAHMLTQTEGPFYETLYGPEPTREELAALYARTAEVRDAVVARFRDAKEMDAALG
                     EDFRVQPPEEKLARLARLGERDSAAVREGALTWPYDPFAEIGDA"
     gene            complement(778..>3347)
                     /locus_tag="VEA63_06315"
     CDS             complement(778..>3347)
                     /locus_tag="VEA63_06315"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004736 - pyruvate carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009374 - biotin binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HYD83644.1"
                     /translation="IAQGHALHSAEVGMPAQADVPRNGFAIQCRITTEDPENKFIPDY
                     GRLLAYRSPGGFGVRLDGAMGFAGAVITPFYDSLLVKCITSGQSYEIALNRARRVLSE
                     FRIRGVKTNIPFLENVIAHPLFRTGQATTMLIDTSPELFSFRPRRDRATKLLNFLGNV
                     IVNGNPHAKGFRPEKSFLAAVPPAAGHGEAPRGTRQMLLELGPKKFAEWTLKQKRLLV
                     TDTTFRDAHQSLMATRVRSYDMLAVASAVAHQAPGLFSLEMWGGATFDTAMRFLNEDP
                     WERLRQLRARVPNICFQMLFRGANAVGYTNYPDHIVAGFVRHAAANGMDIFRIFDSLN
                     YLPNLRVGMEAVQDTHAICEAAICYTGDIMDERRDKFSLQYYVKLAKELERMGAHFLA
                     IKDMAGLCRPYAAHKLVKALREEIGLPIHFHTHDTSGIASASILRASDAGVNVVDLAL
                     ASMSGSTAQPNLNSIVAALQHTPRDTRLDLDRLNEFSDYWEHVREFYRPFDTAPKTGS
                     AEVYLHEMPGGQYTNLKEQAASMGVSHRWPEIARTYAEVNQLFGDIVKVTPSSKVVGD
                     MALFLFSRGIKPADVVNLEPGATPFPESVLDMLEGGLGWPDGGWPEPLWRAVLGEKRF
                     KEAKAKYLAAAKAAAKPRAKSPESRAGVRLSAHGSQLLALRAELSEKLKREATDDELY
                     SHLMYPAVFADFVKHQREFGDVSVLPTPAFFYGLKPGEEISVEIEEGKVLIIRLVSIG
                     APDKDGRRTLSYELNGIAREAFIADKSVAPKTKARVKVDPADPLQIAAPIPGLIVVLS
                     TSVGAKVAKGDKLFMMEAMKMQTTVYAQADGVVAEVNAATGDTVEAKDLIVRLRPAS"
BASE COUNT          600 a         1241 c          912 g          594 t
ORIGIN      
        1 gcgtcgccga tctccgcgaa cggatcgtaa ggccacgtga gcgcgccttc gcgcaccgcc
       61 gcgctgtcgc gctcgcccaa ccgtgcgagt cgcgcgagtt tctcctccgg cggctggacg
      121 cggaaatctt caccgagcgc cgcgtccatt tccttcgcat cgcgaaaccg cgccaccacc
      181 gcgtcgcgca cctccgccgt ccgcgcatac aacgccgcca gttcctcccg cgtcggctcc
      241 ggcccataaa gcgtctcgta aaacggcccc tccgtctgcg tcagcatgtg cgccgccatg
      301 cgctccacca tcgccatcac ctcgggtcgt tcgagataac cctcctcctg ggccttcgcg
      361 atcaccacct gctgcgcgag aaacaaacga aaccaatcgc gcacctcctc caccgatcgc
      421 gcgggccgac ggccgccggt gccgcgaaag cggttcacgt ttttctcgac cgcatacgcg
      481 ctcagttcga gcggaccgat cgtcagcaca atccgccggt cgacttcgac tttcgctccg
      541 ggcggcgcaa tccccggcgc caacatcgtc gccgcgtcga tcaagggcga acacgcaaga
      601 attcccatca accagcaaat ccgtttcacc atgcttttcg ctcaggcaaa accctggcga
      661 aagcgagcca agaaactcag cccgctccgc gtagcggcag gcctccgtgc ctgcctgctt
      721 caacgctcgc aagcaggcag gcacagaagc ccgccgggat ggcgggctcc acctgcctca
      781 actcgccggc cgcaagcgca cgatcaaatc cttcgcctcg accgtgtcgc ccgtcgccgc
      841 attaacctcg gcgaccacgc catcggcctg tgcgtaaacc gttgtttgca tcttcatcgc
      901 ttccatcatg aagagcttgt cgcccttcgc gactttcgcg ccgaccgagg tcgatagcac
      961 cacgatcaaa cccgggatcg gcgccgcgat ctgcagcgga tcggccggat cgactttcac
     1021 gcgcgccttc gtcttcggtg ccacgctctt gtcggcgatg aacgcctcgc gcgcgatgcc
     1081 gttgagctcg tagctcaacg tccgacgccc gtccttgtcc ggcgcgccga tcgagacgag
     1141 gcggatgatc aacaccttgc cctcttcgat ctcgaccgaa atttcctcgc ccggcttcaa
     1201 cccgtagaaa aacgccggcg tcggcagcac gctcacatcg ccgaattcgc gctggtgctt
     1261 cacgaaatcc gcgaacaccg ccggatacat cagatgcgag taaagctcat cgtccgtggc
     1321 ttcgcgcttc agcttctccg acagctccgc tctcaaagcc aagagctgag agccgtgagc
     1381 cgagagcctg actccggctc tggactctgg gctcttcgct ctgggcttcg ccgccgcctt
     1441 cgcggcggcg agatacttcg ccttcgcttc cttgaaccgc ttctcgccga gcaccgcgcg
     1501 ccacaacggc tctggccagc cgccatccgg ccagcccaat ccgccttcca acatgtccag
     1561 cacgctctcc ggaaacggcg tcgcacccgg ctcgaggttc accacgtccg ccggcttgat
     1621 gcctctcgaa aacagaaaca gcgccatgtc gccgacgacc ttcgacgacg gcgtcacttt
     1681 cacgatgtcg ccaaaaagct gattcacctc cgcgtaggtc cgcgcgatct ccggccagcg
     1741 gtgcgacacg cccatcgacg ccgcctgctc cttgagattc gtgtattggc cgcccggcat
     1801 ttcgtgcagg taaacctccg ccgagcccgt cttcggcgcc gtgtcgaacg gccggtaaaa
     1861 ctcgcgcacg tgctcccagt agtccgagaa ctcgttcaac cggtccagat ccagccgcgt
     1921 gtcgcgcggc gtgtgctgca acgccgccac gatcgaattc aaattcggtt gcgccgtgct
     1981 gccgctcatc gaggcgagcg cgagatcgac gacgttcacg cccgcgtcgc tcgcgcgcaa
     2041 aatcgacgcc gatgcgatgc cgcttgtgtc gtgcgtgtgg aaatgaatcg gcaacccgat
     2101 ctcctcgcgc agcgctttca ccagcttgtg cgccgcatac ggccggcaca gccccgccat
     2161 gtccttgatc gccaggaaat gcgcgcccat ccgctccagc tccttcgcca gcttcacgta
     2221 gtattgcagc gagaatttgt cccgccgctc gtccatgatg tcgcccgtgt agcaaatcgc
     2281 cgcctcgcag atcgcgtgcg tgtcctgcac cgcttccatg ccgacgcgca ggttcggcag
     2341 ataattcaac gagtcgaaga tccgaaaaat atccatgccg ttcgccgccg catgacgcac
     2401 gaaacccgcg acgatgtgat ccggataatt cgtgtagccg accgcgttcg cgccgcgaaa
     2461 cagcatctga aaacagatgt tcggcacgcg cgcgcgcagc tgccgcagcc gctcccacgg
     2521 atcctcgttc aggaaacgca tcgccgtgtc gaacgtcgcg ccgccccaca tttccaacga
     2581 aaacaatccc ggcgcctggt gcgccaccgc actcgccacc gccagcatgt cgtagctccg
     2641 cacgcgcgtc gccatcagcg attgatgcgc gtcgcggaag gtcgtgtccg tcaccagcaa
     2701 ccgcttctgt ttcagcgtcc actccgcgaa cttcttcggc ccgagttcga gcaacatctg
     2761 ccgcgtgccg cgcggcgcct cgccatgccc cgccgccggc ggcaccgccg cgagaaacga
     2821 tttctccggg cgaaatcctt tcgcgtgcgg attgccgttc acgatcacgt tgccgagaaa
     2881 attcagcagc ttcgtcgcgc ggtcgcgccg cggccggaac gagaacagct ccggcgaggt
     2941 gtcgatcagc atcgtcgtcg cctgccccgt gcggaacagc ggatgcgcga tcacgttttc
     3001 caagaacgga atgttcgtct tcacgccgcg gatccgaaac tcgctcagca cgcggcgcgc
     3061 gcgattcagc gcgatctcgt agctctgtcc gctcgtgatg cacttcacca gcagcgagtc
     3121 gtagaacggc gtgatcaccg cacccgcgaa acccatcgcc ccgtcgagcc gcacgccgaa
     3181 accgcccggc gaacgatacg ccagcagccg cccgtaatcc gggatgaact tgttctccgg
     3241 atcttccgtc gtgatgcggc actggatcgc gaaaccgtta cgcgggacgt ccgcctgcgc
     3301 cggcatgccg acttcggccg aatgcagcgc atgcccctgc gcgatca
//
LOCUS       DASPLT010000608         1736 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_30973, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000608 DASPLT010000000
VERSION     DASPLT010000608.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1736)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1736)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1736
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_30973"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..514)
                     /locus_tag="VEA63_06320"
     CDS             complement(<1..514)
                     /locus_tag="VEA63_06320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD83645.1"
                     /translation="MPEKSATLPAPEVPPPWADLDGYVFGFAHPGSQLKDHIYERSRR
                     LFAAGDAQRDALQSAETLARHQAKLRADLIDSLGGLPTSTAPLNARVTGVVQAAGFTI
                     EKIIFEARPAHYVTANLYLPAGRATPTAAVLFLCGHHVAAKQQPEYQTVCQTLVAAGL
                     IVFALDPVGQG"
     gene            complement(687..>1736)
                     /locus_tag="VEA63_06325"
     CDS             complement(687..>1736)
                     /locus_tag="VEA63_06325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD83646.1"
                     /translation="GRWAENDPDGIAMHDGLWGAAMGGAAGGSLVWEWDKWVDPQNLY
                     RHFAPLAATLQDVAFNRSIWRAVDVESLVFRDPSCPPYYASVFFEGFSGNYQFKTVPE
                     PRPEVFTLTPEGELDRPEAFDGVLAPVGSAAVPVAGEARNDAAVATRNSQRTLAIDQP
                     RDGELILHVPRLTGEQRPILRVTVDGKQVLEQPLAREREDATWDYFGRFSIPLSAGSH
                     RVVVANVAQAVPDRFWKDRVTVAYELTNYRLRRGPNLDWAGLQSEDTILLWLHNPGFT
                     WMLARIGREPELQPEGVLCLRNVPDGSYTVAWRDTWTGAIVARDTAEAKAGAMELRTP
                     AIAKSAVAKLTRMSL"
BASE COUNT          323 a          557 c          549 g          307 t
ORIGIN      
        1 cgccctgccc caccggatcg agtgcgaaca caatcaatcc cgccgcgacg agcgtctggc
       61 agacggtttg atactcgggc tgctgttttg cagcaacatg atgtccgcac aggaacaaca
      121 ccgccgccgt cggcgtcgcg cgaccggcag gcagataaag gttcgccgtg acatagtgcg
      181 ccggccgcgc ttcgaagatg attttctcga tcgtgaaacc cgcggcctga acgacgcccg
      241 tcacgcgcgc gttgagcgga gcggtcgacg tcggcagtcc gccgagcgag tcgatcaagt
      301 cggcacgaag cttcgcctga tgacgggcaa gcgtctccgc cgactgcaac gcatcgcgct
      361 gcgcgtcacc cgcggcgaac agccggcgcg agcgttcgta gatgtgatcc ttgagctggc
      421 tgcccggatg cgcaaaaccg aaaacataac catcgaggtc ggcccagggc ggcggcactt
      481 caggagcagg aagcgtggcg gatttttcgg gcatgctgtt ttagcggtaa cgtttcagtt
      541 gaaccatgaa tgagaaatca gaaggcgtag ttgtggacct gtcagcgtct ggtcgtcgct
      601 cgatcgtctc gaatacttcg gcatggctcc tctgtcattc gtgtgaattc gtgtccattc
      661 gtggttcgtc agtgcatggt tccggtttag agcgacatcc gggtgagttt ggcgaccgcg
      721 cttttcgcaa tcgccggcgt gcgcaattcc atcgcaccgg cttttgcctc cgccgtgtcg
      781 cgcgccacga tcgcaccggt ccacgtgtcg cgccaggcca ccgtgtagga tccgtcgggg
      841 acgtttcgca aacacagcac gccttcaggc tggagctccg gctcgcgacc gatgcgcgcg
      901 agcatccacg tgaagccggg attgtgcagc catagcagaa tcgtgtcttc gctctgcaaa
      961 cccgcccagt cgagattcgg cccgcggcgc aatcggtagt tcgtcaactc gtaggcgacc
     1021 gtcacgcgat ctttccaaaa gcgatcgggc accgcttgcg cgacgttggc gacgacgact
     1081 cgatgggagc ccgcggagag cggaatcgaa aaacggccaa aataatccca cgtggcatcc
     1141 tcgcgctcgc gcgcgagcgg ctgttcgagg acctgctttc cgtcgacggt cacccggagg
     1201 atcgggcgct gttcgcccgt gagacgcggc acgtgaagga tgagctcgcc atcgcgcggc
     1261 tggtcgatcg cgagcgtgcg ctggctgttg cgcgtcgcca ccgcggcgtc gttgcgcgcc
     1321 tcgccggcga ctgggacagc ggcggacccg accggtgcga gcacgccatc gaacgcttcc
     1381 ggtcgatcaa gctcgccttc gggcgtaagc gtgaacacct cgggccgcgg ctcgggcacg
     1441 gttttgaact gataattgcc ggaaaagccc tcgaagaaaa cgctcgcgta gtaaggcgga
     1501 cacgacggat cgcgaaagac cagcgactcg acgtcgacgg cgcgccagat cgaacggttg
     1561 aaggcaacgt cctgcaacgt cgcggcgagc ggcgcgaaat gacggtagag attctgcgga
     1621 tcgacccact tgtcccattc ccacaccagc gaaccgcccg cggctccacc catcgccgcc
     1681 ccccacaaac cgtcgtgcat ggcgatgccg tcgggatcgt tctccgccca gcggcc
//
LOCUS       DASPLT010000609         1624 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_150548, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000609 DASPLT010000000
VERSION     DASPLT010000609.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1624)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1624)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1624
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_150548"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..343)
                     /locus_tag="VEA63_06330"
     CDS             complement(<1..343)
                     /locus_tag="VEA63_06330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HYD83647.1"
                     /translation="MNKSDGMNYAPRGKPAPVVKPGEFTFAATHLEHGHIYGQCSGLT
                     EAGANLKWVYDADLEKVAAFRAQFPQVKVARSFDEVLADPEVKLVAAAAIPSERGGIG
                     CQVMEVGKDYFT"
     gene            complement(380..>1624)
                     /locus_tag="VEA63_06335"
     CDS             complement(380..>1624)
                     /locus_tag="VEA63_06335"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HYD83648.1"
                     /translation="QQFFNLARDAAMEQLIADKAKATEYGPYTTSVFLVGWAVGGLIF
                     GSLGDRYGRAKILTISVLLYSVFTGLSALSTGFLDFCVYRFFTGLGVGGVFGLVVALV
                     ADSVPDRARAPALGLLQSLSTWGNILAGLIGMGVGLLAARQYLPFGLEAWQTLFLIGA
                     VPAFLCAFIIRKLPEPAKWRDAKAAGALKGVKFGSYATLLRHPKWSRHAWLGLLLCSA
                     GIVGLWGIGNFHPKIVGAIVEQHLAASNLDAESMASEKAYWRALGLLLQNIGGFLGML
                     SLAKYAQVKGRRPAFAIALALSFLSTLLVFKGLREVNQMYWMLPLMGFGQLSVFGVYA
                     IYLPELFPTSLRSTGTSFCYNFGRLAAATAPFTMGKITKSLGGDIESFRTAGVWVSVV
                     LLAGIAVLPFLPETKDEPLPEE"
BASE COUNT          370 a          586 c          412 g          256 t
ORIGIN      
        1 ctgtgaaata atcctttccc acctccatca cctggcagcc gatgccgccg cgttccgacg
       61 gaatcgccgc cgccgccacc agtttaacct cgggatcggc gagcacctcg tcgaacgatc
      121 gcgcgacttt cacctgcgga aactgcgcgc ggaaagccgc caccttctcc agatcggcat
      181 cgtaaaccca tttcaaattc gcccccgcct ccgtgagtcc actgcactgg ccgtagatgt
      241 gaccgtgctc gaggtgcgtc gcggcaaacg tgaactcgcc gggtttcacc acgggagcag
      301 gtttaccgcg aggagcgtag ttcattccgt cggatttatt catgctctaa taggtggatg
      361 cgcgcggcga aaccggaact cattcctcgg gcaatggctc gtccttcgtt tccggcagga
      421 acggcagcac cgcgattccc gccagcaaga ccacgctcac ccacacgcct gccgtgcgga
      481 agctctcgat gtcgccgccg aggctcttcg tgattttccc catcgtgaac ggcgccgtcg
      541 ccgcggcgag acggccgaag ttgtagcaga aactcgtgcc cgtgctgcgc aggctcgtcg
      601 ggaaaagctc cggtaaatag atcgcataca cgccgaagac cgacagctgg ccaaagccca
      661 tcaacggaag catccaatac atctgattca cttcgcgcag gcccttgaag acgagcagcg
      721 tcgagagaaa cgacagcgcg agtgcgatcg caaacgccgg ccggcgtcct ttcacctgcg
      781 catacttcgc cagcgaaagc atcccaagaa atccaccgat gttctgcagc agcagtccaa
      841 gcgcccgcca gtaagccttc tcgctcgcca tgctttcggc atcgagattc gacgccgcga
      901 gatgctgttc gacaatcgcg ccgacgatct tcgggtgaaa atttccaatg ccccacaacc
      961 caacgatgcc cgcgctgcac aacaacaagc ccagccaggc atgccgcgac catttcggat
     1021 gccgcagcag cgtcgcatag gagccgaact tcacgccttt cagcgccccc gccgccttcg
     1081 cgtcccgcca cttcgccggc tcgggcagct tccgaataat aaacgcacag agaaacgccg
     1141 ggacggcgcc gatcaaaaac agcgtctgcc aagcctcgag gccgaacggc aaatactgcc
     1201 gcgccgccag cagccccacg cccatgccaa tcaatcccgc cagaatgttt ccccatgtcg
     1261 acaacgattg cagcaaaccc agcgccggcg cccgcgcgcg gtccggcacc gagtccgcca
     1321 cgagcgccac gaccagaccg aacaccccgc ccacgcccaa acccgtgaaa aaccgataca
     1381 cacagaaatc caggaagccg gtcgacaacg cgctcagccc cgtaaacacg gaatacaaca
     1441 acaccgaaat cgtcaggatc ttcgcgcgac cgtaacgatc gcccagcgat ccgaaaatca
     1501 gcccgcccac cgcccagccc acaaggaaca ccgacgtcgt atacggcccg tattccgtcg
     1561 ccttcgcctt atccgcgatc aactgctcca tcgccgcatc acgcgcgagg ttgaaaaact
     1621 gctg
//
LOCUS       DASPLT010000610         1210 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_30986, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000610 DASPLT010000000
VERSION     DASPLT010000610.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1210)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1210)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1210
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_30986"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..647
                     /locus_tag="VEA63_06340"
     CDS             <1..647
                     /locus_tag="VEA63_06340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HYD83649.1"
                     /translation="ADEKRGAARGINVIPGMTFAANEKPTVDAVIATLGEQVVVKPND
                     QGSSVGLRVVGSRAELVEALAAITTGNWLIEKRVFGRELAVGVLMGRPLGIVEIRPKS
                     GIYDYASKYTKGLTEYLAPAPLSAELTVAIRAAAATAFAACGCRDYARVDFMIGENDA
                     FYLLEINTLPGMKETSLLPMSARCEGLDFNELVRDMVAPALQRFHAAASPVLST"
     gene            644..>1210
                     /locus_tag="VEA63_06345"
     CDS             644..>1210
                     /locus_tag="VEA63_06345"
                     /inference="COORDINATES: protein motif:HMM:NF020067.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HYD83650.1"
                     /translation="MNRSEINAPPARSWRDIPQQVKPRAMSTEGRRRVWWSATKTVLA
                     VAFVGGLGWGGFQIAAALRGNPNLLADHAEAVPVKEVELVTDGVLEKTWLVPTLALPK
                     GATLMELDLYQLQERVMATTQVRAATLTRNFPATLTVTIAEHSPVARVMAQVGDAPPR
                     ALLVAREGVVFEGHGFDPEMIETLPWLAG"
BASE COUNT          218 a          295 c          481 g          216 t
ORIGIN      
        1 acgctgacga aaaacgcggg gcggcgcgcg ggatcaacgt gattcctgga atgacgttcg
       61 cggcgaacga gaagccgacg gtggacgcgg tgatcgcgac gctcggcgag caggtggtgg
      121 tgaagccgaa cgatcagggg agcagcgtgg gattgagagt cgtgggctcg cgcgcggagt
      181 tagtggaggc actggcggcg atcacgacgg gaaactggtt gatcgagaaa cgcgtgttcg
      241 ggcgcgaact ggcggtgggc gtgttgatgg ggcgtccgtt ggggatcgtg gaaatccggc
      301 cgaagtcggg gatctacgat tacgcgagca aatacacgaa agggctgacg gagtatctcg
      361 cgccggcgcc gttgtcggcg gagttgacgg tggcgattcg cgcggcggcg gcgacggcgt
      421 tcgcggcgtg cgggtgtcgc gattatgcgc gcgtggattt catgattggt gagaacgacg
      481 cgttttattt gctggaaata aacacgctcc ccggcatgaa agaaacgagc ttgctgccga
      541 tgagcgctcg ctgcgagggg ctggatttca acgagctggt cagggacatg gttgcacccg
      601 cgctgcagcg ttttcatgcg gcggcgtcgc ctgttttatc gacgtgaacc gaagcgagat
      661 caacgccccg cctgcgcgca gttggagaga cattccccaa caggtgaagc cgcgtgcgat
      721 gtcgacggaa ggccggcggc gcgtgtggtg gagcgcgacg aagacggtgc tcgcggtggc
      781 gttcgtcggc gggctcggct ggggtggttt tcaaatcgcg gcggcgttgc gcggaaatcc
      841 gaacctgctg gcggatcacg cggaagcggt gccggtgaag gaagtcgagc tggtgaccga
      901 cggcgtgttg gagaaaacgt ggctggtgcc gacgctcgca ctgccgaaag gcgcgacgtt
      961 gatggagctg gatctttatc aattgcagga gcgggtgatg gcgacgacgc aggtgcgcgc
     1021 ggcgacgttg acgcggaatt ttccggcgac gttgacggtg acgattgcgg agcattcgcc
     1081 ggtggcgcgg gtgatggcgc aggtcggcga cgcgccgccg cgggcattgt tggtcgcgcg
     1141 agaaggcgtg gtgttcgaag ggcatggctt cgatccagaa atgatcgaaa cgctgccgtg
     1201 gctggcgggc
//
LOCUS       DASPLT010000611         1325 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_46882, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000611 DASPLT010000000
VERSION     DASPLT010000611.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1325)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1325)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1325
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_46882"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            14..1288
                     /locus_tag="VEA63_06350"
     CDS             14..1288
                     /locus_tag="VEA63_06350"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007359927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HYD83651.1"
                     /translation="MRPDWTDVRAVAEYLFGLKPRGTKFGIDRMRLLAAKLGYPERAL
                     PVIHIAGTNGKGSVAAMLEAILREAGWKVGLYTSPHLVWVGERVQVDREALSASELAA
                     YVAELDAIADEIATEVGEDDRPSFFEYMTAMAFLQFVRKRCDIAVVEVGLGGELDATN
                     IVTPEVSVITSIALDHCEYLGETIEKIAVAKAGIIKNGRPLVVGRLPVEAEPVIRTHA
                     AVKGAAVRSVREEFGEEVARYPRTRLAGEYQRWNAATAALVAQALGARWKISDEIITR
                     GLQSVEWTGRWEQVTLAGRAIVLDASHNAEGAAVLEKNLEELEKKTGRKPVVIVGVLG
                     GQRAQPLMETIARHAREIHLVVPNQSRATSYEELAAFVPRDYAGPILRRTVANVFPGG
                     EICAAGGADDVVVVTGSIYLLGEVLSRIRIKS"
BASE COUNT          262 a          339 c          478 g          246 t
ORIGIN      
        1 agttgatcgt cgcatgcggc cggattggac cgatgtccgc gcggtggcgg agtatctgtt
       61 cggcctgaaa ccgcgcggaa cgaagttcgg tatcgaccgc atgcgtttgc tggcggcgaa
      121 gctcgggtat cccgaacgag cgctgcctgt gattcacatc gcgggcacga atggcaaagg
      181 ctcggtcgcg gcgatgctcg aagcgatcct gcgcgaagcc ggttggaagg tgggactgta
      241 tacgtcgccg catctcgtgt gggtgggcga acgcgtgcag gtggatcgcg aggcgctgag
      301 cgcgagcgag ctcgcggcct acgtggcgga actcgacgcg atcgcggacg agatcgcgac
      361 agaagtcggt gaggacgacc ggccgagttt tttcgaatat atgacggcga tggcgtttct
      421 gcagtttgtg cggaagcgtt gcgacatcgc ggtggtggaa gtcgggctcg gcggcgaact
      481 cgatgcgacg aacatcgtga cgccggaggt ttccgtgatc acttcgatcg cgctggatca
      541 ctgcgaatac cttggcgaaa cgatcgagaa gatcgcggtt gccaaagcgg gtatcatcaa
      601 aaacggccgg ccgctcgtgg tcggacgtct gccggtagaa gcggaacccg tgatccgcac
      661 gcacgccgcg gtgaagggag cggcggtgag gtccgtgagg gaagaatttg gcgaggaggt
      721 tgcacgttat ccgcgcacgc gactcgctgg cgagtatcag cgctggaatg ccgcgacggc
      781 ggcactcgtg gcgcaggcgc ttggtgcgcg ctggaaaatc agtgacgaaa tcatcacgcg
      841 cggattgcag tcggttgagt ggacggggcg ttgggagcag gtgacactag ccgggcgggc
      901 aatcgtattg gatgcgtcgc acaacgcgga aggtgctgcg gtgctcgaaa agaatctgga
      961 ggaactggaa aaaaaaacgg gacggaaacc ggttgtgatc gtcggtgtgc tcggcggcca
     1021 gcgagcgcaa ccattgatgg agacgatcgc gcgccatgcg cgtgagattc acctcgttgt
     1081 tccgaatcag tcacgtgcga cttcttacga ggaactcgct gcgtttgtgc cgcgcgacta
     1141 cgcggggccg attctgcggc gaacggtcgc gaacgttttt ccgggcggtg agatttgcgc
     1201 ggcgggcggt gcggatgacg tcgtggtcgt gaccggctcg atctatctcc tcggcgaagt
     1261 gctttcgcgg atacgaatca agtcatagcc acagaagccg taaaaagcgc aaaattcccc
     1321 agtag
//
LOCUS       DASPLT010000612         1067 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_294466, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000612 DASPLT010000000
VERSION     DASPLT010000612.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1067)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1067)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1067
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_294466"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(136..507)
                     /locus_tag="VEA63_06355"
     CDS             complement(136..507)
                     /locus_tag="VEA63_06355"
                     /inference="COORDINATES: protein motif:HMM:NF012305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HYD83652.1"
                     /translation="MTNIFVGNLPYTTTEDELHSAFGAYGQVDRVSIVRDRETGQPRG
                     FAFVEMPNASEAEQAINGMNGRDMNGRALNVNEARPREDRGGARGFGGGGGGNRAGGG
                     GGGGNRGGNGGGFNRRREPRW"
     gene            806..>1067
                     /locus_tag="VEA63_06360"
     CDS             806..>1067
                     /locus_tag="VEA63_06360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD83653.1"
                     /translation="MVPVLCVAATCESPFTATVTPGSRLRLDIRPGNIEIRGAEGDGL
                     RVTCAFKDGTPPGAISVSFQNGELKVSGGPTGGNSSATIRIDV"
BASE COUNT          197 a          335 c          329 g          206 t
ORIGIN      
        1 gaaagagccg gaggttttgg ccgctcgcgc tcgtttcgag aagattatgg aagggctgcc
       61 ggcaccgaag cgcgagaagg ccggctgagg ctgacagagg gcgggtttgc tagaaagcag
      121 cccgccccgg aagccttacc agcgaggttc gcggcgccgg ttgaacccgc cgccattgcc
      181 gccgcggttt ccaccgccac cgccaccgcc ggcacggttg ccaccgccgc cgccgccgaa
      241 gccacgagcc ccgccgcggt cttcgcgcgg acgtgcttcg ttgacgttca gtgcgcgacc
      301 gttcatgtcg cgcccgttca tcccgttgat cgcctgctcg gcttcagaag cgttgggcat
      361 ctcgacgaac gcaaaaccgc gcggctgccc cgtttcacgg tcacgaacaa tgcttacacg
      421 gtccacttgg ccataagcgc caaaagcgga gtgaagctcg tcttcagttg tcgtataggg
      481 aaggtttccc acaaagatgt tggtcactca aatactccaa aaaggctgta tttacacagc
      541 tgtccgtcta tgctgccaac cacggcagcg gttaatcaca ggcgagctag tctgggccgg
      601 agagaaacta caggcgggta aacgtaacac gcaggaataa cgccactcct taggcggcaa
      661 tctcagcata ccacaaaagc ggggaatcca cgcgttggtt gcggggtgtg tggctgtcgg
      721 tggcgcctcg gccgctggcg ttcatttggt tgaactccgt acccatttgt gcgtatcgtt
      781 gggcatgcga ctctggttgt ctctcgtggt tcccgtcctg tgcgtcgccg cgacgtgcga
      841 gtctccgttc acagccaccg tcacgcccgg atcacgcctc cggctcgata tccggcccgg
      901 caatatcgag atccgcggtg ccgagggtga cgggcttcgg gtcacgtgcg cctttaagga
      961 cggcacgccg cccggtgcga tctcggtgtc ttttcaaaac ggcgaactca aggtctccgg
     1021 cgggccgacc ggcggcaata gcagtgcgac gattcgcatc gacgtgc
//
LOCUS       DASPLT010000613         1247 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_212326, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000613 DASPLT010000000
VERSION     DASPLT010000613.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1247)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1247)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1247
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_212326"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..746
                     /locus_tag="VEA63_06365"
     CDS             <1..746
                     /locus_tag="VEA63_06365"
                     /inference="COORDINATES: protein motif:HMM:NF012958.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HYD83654.1"
                     /translation="PRSRVVQIFLGVALALMVVDDASAARRSPQLTARPAQPTEQPIR
                     GLHNLGLGNRRDGFVYVPENYNPAQAAPLMVLLHGAGGTAKPGWMPYTKMAESRGIIL
                     LAIDSRSHRTWDGILDAWGPDVSFIDEALARIFAHYHVDAARIALVGFSDGASYALSL
                     GAANGDLFTHVVAFAPGSIRPVMTRGRPRIFVSHGHNDPVLSFQRTRDGIVPTLQRTG
                     YDVTFRDTNSDHEVSWDISIEMMDWFLAR"
     gene            complement(782..>1247)
                     /locus_tag="VEA63_06370"
     CDS             complement(782..>1247)
                     /locus_tag="VEA63_06370"
                     /inference="COORDINATES: protein motif:HMM:NF012363.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carboxylesterase family protein"
                     /protein_id="HYD83655.1"
                     /translation="GERIAAFRALYPVSNDVEAGAAQKQRGRDAARADLYAWTALRAK
                     HGRAKDWGYYFDRAIPWPEHPEYQAFHSGELPYTFNNLRLMKRPWEEVDHRLAEQVSN
                     YWVNFINTGNPNGSGLLHWPDDNGQLMRLGRDVVPEPVLSAEKLEFYLGDSR"
BASE COUNT          211 a          443 c          349 g          244 t
ORIGIN      
        1 gccctcgatc tcgtgtcgtc caaatttttc tcggtgtcgc actcgcgctt atggttgtcg
       61 acgatgcgag cgccgcccgg cggtctccgc agctcacggc ccggccggcc caacccaccg
      121 agcaaccgat ccggggactg cacaatctgg gcctcggaaa ccggcgcgac ggcttcgtgt
      181 atgtgccgga aaactacaac ccagcccaag ccgcgccgtt aatggttctg ctccacggcg
      241 ccggcggcac cgcgaaaccc ggctggatgc cctacacgaa aatggccgag tcgcgcggca
      301 tcatcctgct cgcgatcgac tcgcgttcgc atcgcacttg ggacggcatc ctcgatgcct
      361 gggggcccga cgtgagtttc atcgatgaag cactggcgcg gatcttcgcg cattatcacg
      421 tcgacgcggc gcgcatcgcg ctggtgggct tttccgacgg cgcctcttat gcgctttcgc
      481 tcggcgcggc gaatggcgat ctcttcactc acgtcgtcgc gtttgccccc ggctccattc
      541 gtcccgtgat gactcgcggc cgcccgcgca tcttcgtctc ccacggccac aacgaccccg
      601 ttctctcttt ccaaagaacg cgcgatggca tcgtgccgac cctgcaacgc accggctacg
      661 atgtcacttt tcgcgacacc aactccgatc acgaggtgag ctgggatatt tcgatcgaga
      721 tgatggactg gtttctggcg cggtgatctc acgccccacg cttcgcccac cctcgtctgc
      781 gtcaccgcga gtcgccgaga taaaactcga gcttttccgc actcaacacc ggttccggca
      841 cgacgtcccg gccgagccgc atgagctgcc cgttgtcgtc aggccagtgc agcaggccgg
      901 agccgttggg gtttcccgtg ttgataaaat tgacccaata gttcgacacc tgctccgcga
      961 gccgatggtc aacctcttcc cacggccgtt tcatgaggcg cagattgttg aacgtgtaag
     1021 gcaactcgcc cgaatgaaat gcctgatact ccgggtgctc cggccacgga atcgcgcggt
     1081 cgaaataata gccccaatcc ttggccctgc cgtgtttcgc gcgcagcgcc gtccacgcat
     1141 acaagtccgc tcgcgcagcg tcccgcccgc gctgcttctg cgccgcaccg gcctccacgt
     1201 cgttggaaac cggatacaac gcacgaaacg ccgcgatccg ctcgccg
//
LOCUS       DASPLT010000614         1658 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_294471, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000614 DASPLT010000000
VERSION     DASPLT010000614.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1658)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1658)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1658
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_294471"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..830
                     /locus_tag="VEA63_06375"
     CDS             <1..830
                     /locus_tag="VEA63_06375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HYD83656.1"
                     /translation="NLGNGEFRSRTILDRAARVSDVRAADFDGDGRLDLVVGQFGYEQ
                     GEIRWMRNRGDWEFESHVISGLSGTIHVPIVTLHGGTQPGFLALVSQEYEEIHSFNNA
                     GGELVDTLIWGSINEDYGSSGLDVADVNADGRIDIIYTNGDAFDYARPGPRPWHGIQW
                     LENRGDGGFVFHRVGQLPGAYSPCAADLDGDGSIDLLAVSGFNDWSNPGAVSMMVWLN
                     DGQQRFTPVPLAQAPTHLITAAVGDLDGDGVPEIVTGGFHAYPPYKHMSRLTLWKRQ"
     gene            851..>1658
                     /locus_tag="VEA63_06380"
     CDS             851..>1658
                     /locus_tag="VEA63_06380"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HYD83657.1"
                     /translation="MRRTPLSAIAKRLGLLLVAGLLLAAAVGVGLWLRTRSHGIDPEQ
                     VRAATPAIPDLAGWPREFAAKVRAATAAAERREQPLRALSELAFLYHANGFFREAEQV
                     ERGLHALEPRNADWLYLLADVCHSIGDMAGTEQFLARTVELDPKHHVAHLKLADLLFK
                     RGQASAAARHYETRLALVPRDPYARVGLARIALYQRDGDGARRQLEWIATHCPDFPTA
                     HNVLAGIYEQTGEHRRAAEERERGLNAGRFRAADDPLIDRLYAWSCDPYRL"
BASE COUNT          275 a          504 c          565 g          314 t
ORIGIN      
        1 aaaacttggg caatggggaa ttccgctcgc gaacgatcct ggatcgcgcg gcgcgggtgt
       61 ccgatgttcg cgcggccgac ttcgatggcg acgggcgtct cgatctggtg gtcggccaat
      121 tcggctacga gcaaggtgaa atccgttgga tgcggaatcg cggcgattgg gagttcgaaa
      181 gccacgtgat cagcgggctc tcgggcacga ttcacgtgcc gatcgtgact cttcacggcg
      241 gaacgcagcc cggttttctc gcgctggtgt cgcaggagta tgaagaaatc cactccttca
      301 acaacgccgg cggcgagctc gttgacaccc tgatctgggg ttcaatcaac gaggactacg
      361 gcagcagcgg actcgacgtc gccgacgtca acgccgacgg tcgtatcgac attatttata
      421 cgaacggcga tgccttcgac tacgcccgcc cgggtccgcg gccgtggcac ggcatccagt
      481 ggctcgagaa tcgcggcgac ggcggattcg tgtttcatcg ggtcggccag ctgccgggcg
      541 cgtattctcc gtgcgccgcc gatctcgacg gcgacggctc gatcgatctt ctggcggtga
      601 gcggtttcaa cgactggtcg aaccccggcg cggtttcgat gatggtgtgg ctgaacgacg
      661 gccagcagcg gttcacgccc gtgccgctgg cgcaggcgcc gacgcatctc atcacggccg
      721 cggtcggcga tctcgatggc gatggcgttc cggaaatcgt gaccggcggg tttcatgcgt
      781 atccgcctta caagcacatg agccggctga cgctctggaa acggcagtag gctaaacgcg
      841 tttacgcccg atgcgccgca ctcctttatc cgccatcgcg aaacgcctgg gcttgttgct
      901 cgtcgcggga ttgctgctcg cggcggccgt cggcgtcggg ctttggctgc ggacacgctc
      961 gcatggcatc gatccggagc aggtgcgggc ggcaacgccg gcgattcccg atctggcggg
     1021 ctggcctcgg gagttcgcgg cgaaagttcg tgctgcgacc gctgcggcgg aacgcaggga
     1081 gcaaccgctc cgcgcgttgt cggaactcgc tttcctatat cacgccaacg gtttttttcg
     1141 cgaagccgaa caggtggagc gcggattgca cgcgctcgaa ccgcgaaacg cggactggct
     1201 ctatctgctg gcggatgttt gccacagcat cggcgacatg gcgggcacgg agcagtttct
     1261 cgcccgaacg gtcgagctgg atccgaagca tcatgtcgca cacctgaagc tcgcggattt
     1321 gttgttcaag cgagggcagg cgagcgcggc ggcgcgacac tacgagacgc ggctcgcgct
     1381 cgtcccgcgc gatccgtatg cgcgcgtcgg gctggcgcga atcgccttgt atcagcgcga
     1441 tggcgacggc gcccgccggc agctcgagtg gatcgcgacg cactgccctg attttccgac
     1501 tgcgcacaat gtgctcgcgg ggatttatga acaaacgggc gagcatcgtc gcgccgcgga
     1561 ggaacgggag cgcggactca acgcggggcg tttccgtgcg gccgatgatc cgttgatcga
     1621 tcgcctttat gcgtggagtt gcgatccgta tcggctcg
//
LOCUS       DASPLT010000615         1127 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_130453, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000615 DASPLT010000000
VERSION     DASPLT010000615.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1127)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1127)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1127
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_130453"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1127)
                     /locus_tag="VEA63_06385"
     CDS             complement(<1..>1127)
                     /locus_tag="VEA63_06385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009508167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HYD83658.1"
                     /translation="AVRSTMAELKKNFPDGLDYDIVYDPTVFVRDSIKAVVHTLLEAV
                     VLVVIVVILFLQTWRASIIPLVAVPVSLVGTFAVMHALGFSINALSLFGLVLAIGIVV
                     DDAIVVVENVERNIALGKSPLEATKQAMREVTGPIVATALVLSAVFIPTAFITGLSGQ
                     FYKQFAITIAISTVISAFNSLTLSPALSALLLRDHHAPKDRLSRIMDRSLGWFFRPFN
                     RFFEWSAQKYSRGVARLIRLTAVSLIVYVGLLAFTGFSFNKIPSGFVPSQDKQYLIAF
                     TQLPSAASLDRTDDVIRRMSDIAMKHPGVSNAVAFPGLSINGFTNSSNSGIVFVILKS
                     FEERKSDELSGNAIAAALQQQFGEIQDAFIAIFPPPPVMGL"
BASE COUNT          244 a          327 c          375 g          181 t
ORIGIN      
        1 gaggcccatc accggcggcg gtgggaagat cgcgatgaac gcgtcctgga tttcgccgaa
       61 ctgctgctga agcgccgccg cgatcgcatt ccccgagagc tcgtcgcttt tgcgttcctc
      121 aaaggacttc aggatcacaa acactatgcc gctgttcgac gagttggtga agccgttgat
      181 cgagaggccg gggaaagcga cggcgttgga gacgcctgga tgcttcatcg cgatgtcgct
      241 catgcggcgg atcacgtcgt cggtgcgatc gagcgatgca gcggacggca gctgcgtgaa
      301 cgcgatcagg tattgtttgt cctgcgacgg cacgaagccg ctcgggattt tgttgaacga
      361 gaaaccggtg aacgcgagca ggccgacgta gacgatcagc gacacggccg tgagccggat
      421 caaccgcgcg acgccgcggg aatacttctg cgccgaccat tcgaagaagc ggttgaacgg
      481 acggaaaaac cagccgagcg aacgatccat gatgcgcgag agccggtcct tcggcgcatg
      541 gtgatcgcgc agcaggagcg cgctcagcgc gggcgaaagc gtgagcgagt tgaaggcaga
      601 gatcaccgtc gagatcgcga tcgtgatcgc gaactgcttg tagaattgac cgctgaggcc
      661 ggtgatgaag gccgtcggaa taaagaccgc ggagagcacg agcgccgtgg cgacgatcgg
      721 cccggtcacc tcgcgcatcg cctgcttcgt cgcttcgagc ggcgacttgc cgagcgcgat
      781 gtttcgctcg acgttttcca ccaccacgat cgcgtcgtcg accacgatgc cgatcgcgag
      841 cacgagcccg aagagcgaca gcgcgttgat cgagaaaccg agcgcgtgca tcacggcaaa
      901 cgtgccgacg agcgacaccg gcaccgcgac gagcgggatg atcgacgcgc gccaggtttg
      961 gagaaagaga atcaccacga tgacgacgag gaccaccgcc tcgagcagcg tgtgaacgac
     1021 ggccttgatc gaatcgcgga cgaaaaccgt cggatcgtag acgatgtcgt aatcgagccc
     1081 gtcgggaaaa ttcttcttca gctccgccat cgtggagcgc acggcgt
//
LOCUS       DASPLT010000616         1067 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_46900, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000616 DASPLT010000000
VERSION     DASPLT010000616.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1067)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1067)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1067
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_46900"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..790
                     /locus_tag="VEA63_06390"
     CDS             <1..790
                     /locus_tag="VEA63_06390"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HYD83659.1"
                     /translation="LTFHGSEILKFAHSPWRRGLARRLIRHATRVTTLTNYTQELLLE
                     HFPEAADKISLTPGALRTDFVVVPRKLPDPGDKIIVLTVGRLHPRKGQLLTLHALQAL
                     APEVRARLEYWIVAGHSRSHYESTLRETAALTPDLTVRFLGNLPDNELSDVYDRADIF
                     AMTSINHGRSIEGFGLVYLEASAHGLPIVAHDVGGVSEAVVNDVTGLLVPPNRPAQLA
                     AAFERLVFEPALRRRLGDAGREWATRNCWTRSAAALFEPVEQPA"
     gene            787..>1067
                     /locus_tag="VEA63_06395"
     CDS             787..>1067
                     /locus_tag="VEA63_06395"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009509426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HYD83660.1"
                     /translation="MIQSRATVTVRYAETDMMGVVYHANYLPWFEVARTQLLREQGFP
                     YRQLEADGFRIPVLEVTAKYLRPAVYDDTLTIVATIREKPLLRIRIDYEV"
BASE COUNT          192 a          380 c          293 g          202 t
ORIGIN      
        1 gcttacgttt cacggctcag agatcttgaa atttgcacac agcccgtggc gtcgcggact
       61 cgcgcgtcgc ttgatccgtc atgcgacccg cgtgaccacg ctcacgaact acacgcagga
      121 gcttctgctc gaacatttcc cggaagctgc cgacaaaatt tccctcacgc ctggcgcgct
      181 gcgcaccgat ttcgtggtcg tgccgcggaa gctgccagat cccggcgaca aaatcatcgt
      241 cctgacggtc ggccgcttgc atccgcgcaa gggccagctc ctcacgctgc atgccctcca
      301 agctctggcg cccgaagtgc gcgcgcgctt ggaatactgg atcgtcgccg gccacagccg
      361 gagccactat gaatcaacgt tgcgcgaaac cgccgcgcta acgcccgacc tgaccgtgcg
      421 cttccttggt aatctgcccg acaacgaact ttccgacgtt tacgaccgcg ccgacatctt
      481 cgcgatgacg agcatcaatc atgggcgcag catcgaaggt ttcggtctgg tctatctcga
      541 agcctccgct cacggcctgc cgatcgtcgc ccacgatgtt ggcggcgtgt ccgaggccgt
      601 cgtgaacgac gtcaccggtt tgctcgtccc gcccaatcgc cccgcgcagc tcgcggccgc
      661 cttcgagcgg ctcgtcttcg aaccggcact gcgccgtcgt ctgggcgatg cgggccgcga
      721 atgggccaca cgcaattgct ggacgcgttc cgccgccgcg ctcttcgagc cggttgaaca
      781 acccgcatga tccaatcacg cgccaccgtc accgtccgct acgccgaaac cgacatgatg
      841 ggcgtggtct accacgccaa ttatctcccg tggttcgaag tcgcgcgcac ccagcttttg
      901 cgcgagcaag gattccccta tcggcagctt gaagcggatg gcttccgcat ccccgtgctc
      961 gaagtcactg cgaaatacct gcggcccgcc gtctacgacg acaccctcac gatcgttgcg
     1021 acaattcgcg aaaaaccgct gctgcgcatc cgcatcgatt acgaggt
//
LOCUS       DASPLT010000617         1286 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_192341, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000617 DASPLT010000000
VERSION     DASPLT010000617.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1286)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1286)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1286
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_192341"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..379
                     /locus_tag="VEA63_06400"
     CDS             <1..379
                     /locus_tag="VEA63_06400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HYD83661.1"
                     /translation="NLSRRERRDRVAMALGLVGLAERMEHLPSELSGGQQQRVAIARA
                     IITDPTLLVADEPTGDLDRVSAEEILELLDRLNRELGKTIIMVTHDKRAADKAHAIMY
                     LEKGELSTAEGIAERSVRESRHA"
     gene            380..970
                     /locus_tag="VEA63_06405"
     CDS             380..970
                     /locus_tag="VEA63_06405"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HYD83662.1"
                     /translation="MLHFLKLILRNGMRHKLRSALTVLGLVVAILSFGLLQTIVDAWY
                     KGADAAAANRLVTRNAVSLVFPMPIHYRERIRAVEGVEGVAAANWFGGIYRDTSVANF
                     FPKFAVDPEPYFAMYPEYVLDPDQFRAFQRDRKGAIIGRKLALNHGFRIGEVIPVQGD
                     IFPGNWEFTVRGIYDAGQASTDTSLMFFTTNTSTRR"
     gene            1030..>1286
                     /locus_tag="VEA63_06410"
     CDS             1030..>1286
                     /locus_tag="VEA63_06410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HYD83663.1"
                     /translation="MGRAAEIARDIDAQFKNSSAETLTETEKAFQIGFAKQTEAIVIA
                     VRFVSYVVIVIILAVMANTMSMTARERMAEYATLKALGFQPA"
BASE COUNT          232 a          416 c          415 g          223 t
ORIGIN      
        1 caatctttcg cgacgcgagc ggcgcgaccg cgtcgccatg gcgctcgggc tcgtcggcct
       61 cgcggagcgc atggaacatc tgccctcgga attgtcgggt gggcagcagc agcgcgtggc
      121 gatcgcgcgc gccatcatca ccgatccaac tttgctggtc gcggacgagc cgaccggcga
      181 tctcgaccgc gtgtccgccg aagaaatcct cgaactgctc gatcggctga accgcgagct
      241 gggcaagacc atcatcatgg tcacgcacga caagcgggcg gccgacaagg cgcacgcgat
      301 catgtatctg gagaagggcg agctctcgac ggcggagggc atcgccgagc gatcggtgcg
      361 cgagagccgc cacgcgtaga tgctgcattt cctcaagctg atcctgcgca acggcatgcg
      421 gcacaagttg cgcagcgcac tcacggtgct gggcctggtc gtcgcaatcc tgtccttcgg
      481 gttgctgcaa acgatcgtgg acgcctggta caagggcgcc gatgccgcgg cggcgaaccg
      541 tctggtgacg cgcaacgcgg tctcgctggt gttccccatg cccatccact atcgcgagcg
      601 gatccgcgcc gtggagggcg tcgagggcgt ggccgcggcg aactggttcg gcggcatcta
      661 ccgcgatacc agcgttgcca atttctttcc gaaattcgcc gtcgacccgg agccctattt
      721 cgcgatgtat ccggaatacg tgctcgatcc ggatcagttc cgggctttcc agcgcgaccg
      781 caagggcgcg atcatcggcc gcaagcttgc gctcaatcac ggctttcgca tcggcgaagt
      841 cattcccgtc cagggcgaca tctttccggg caactgggaa ttcaccgtgc ggggcatcta
      901 cgacgcgggc caggcgagca ccgacacgtc gctgatgttc ttcactacga atacctcaac
      961 gagacgctga agcgccgcgc gccgtctcgc gcggacaagg tcggcgtctt cgtcgtctcg
     1021 atcgacgacg tggggcgcgc cgcggaaatc gcgcgcgaca tcgacgcgca gttcaagaac
     1081 tcatcggccg agacgctcac ggagacggag aaggcattcc agatcggctt cgcgaaacag
     1141 acggaagcga tcgtcatcgc ggtgcgcttc gtgtcctacg tcgtgatcgt catcattctc
     1201 gccgtcatgg cgaacaccat gtcgatgacc gcgcgcgagc gcatggcgga atacgcgacg
     1261 ctgaaggcgc tcggtttcca gccggc
//
LOCUS       DASPLT010000618         2526 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_46911, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000618 DASPLT010000000
VERSION     DASPLT010000618.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 2526)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2526)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2526
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_46911"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1661
                     /locus_tag="VEA63_06415"
     CDS             <1..1661
                     /locus_tag="VEA63_06415"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HYD83664.1"
                     /translation="LIGLRMSNALMTDATTRELVDHLGAFQSYGVNTISVYVMGSRFG
                     DVKGFRPDASLDPVYAERLARIVAAADERGMIVLVGCLYWSESEAKAELGHWTQAEAN
                     RAVANVVRWLKERNFRNVFVDPDNEGMAHKEKGWSIAEMIDAGHRENPACVIAYNARP
                     TPPANADLTIHHAPRVAGKPYIETEGTPQVVPYWHAYSRREGLRNYLNIGIYSEAMKA
                     EQRRDTDEGIANANGFMLASTWLQCPPPFGPNMRPEGDGSPENPGILWWLEHVKARHG
                     AGTAMRASWPFFAMDNGVGRGVWTPAQQASTLRELGFDGISYNYTNVAELRVWLSELR
                     RQELKLYALYFPVALQGTAPLPDGFAEAVDLLRGSGTALWMLIPAKSRPAGYEAMAVE
                     RVREVADLAADAGLRVVLYPHVQCVPATAEEAFSLSEQAERANVALTMNLSHELAAGN
                     GARLTEIVRHVVPRLAMVSINGATDEPGPLWKNYIRVLGEGDYDVAGLLRTLRDVDYG
                     GPVGLQSYGIEGKPGDNLAASMRAWKALTEESSRERQERRRPKT"
     gene            1729..>2526
                     /locus_tag="VEA63_06420"
     CDS             1729..>2526
                     /locus_tag="VEA63_06420"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HYD83665.1"
                     /translation="MLAARVAPQIVSSRLFGATAPSNRVTLGCIGLGVHGFGVNLKSF
                     LYQDDCVVRAVCDVFGERREQGRTAVDERYGNRDCAVFEDFRELLARPDIDAVVISTP
                     DHWHVAMSLLALEAGKMVFCEKPTLTIAQGRRLADEVKRRGLLFATGLEDRAELKYHL
                     LAEAVRNGAIGTLQRIQIGLPEKPVFAKEEPAPVPADLNYELWLGPARFRPYTPNLTD
                     AQVWRQIRDFSGGSLTDWGAHLIDTAQVANFSEDTSPVAVEGAGEIPA"
BASE COUNT          459 a          648 c          941 g          478 t
ORIGIN      
        1 agttgatcgg actgcggatg tcgaacgcgc tgatgaccga tgcgacgaca cgggaactcg
       61 tcgatcatct cggcgcgttt cagtcctacg gcgtgaacac gatttcggtt tacgtgatgg
      121 gttcgcgatt cggcgacgtg aaaggattcc ggcccgacgc gagcctcgac ccggtttacg
      181 cggagcggct ggcgcggatc gtggcggccg cggatgagcg cgggatgatt gtgctggtgg
      241 gctgtctgta ttggtcggag tcggaggcga aggcggagct cggtcactgg acgcaagcgg
      301 aagcgaaccg cgcggtggcg aatgtggtgc gctggttgaa ggagcggaat tttaggaacg
      361 tgtttgtcga tccggacaat gaaggcatgg cgcacaagga gaagggttgg agcattgcgg
      421 agatgatcga tgcggggcat cgcgaaaatc cggcgtgtgt gatcgcttac aatgcgcgtc
      481 cgactccgcc ggcgaacgcc gacctgacga ttcatcacgc gccgcgcgtg gcggggaaac
      541 cttacatcga gaccgaaggc acgccgcagg tggttcctta ctggcacgcg tatagccgtc
      601 gcgaaggttt acggaactat ctcaacatcg ggatttactc ggaagcgatg aaggcggagc
      661 agcggcgcga taccgacgag ggaatcgcaa acgcgaatgg tttcatgctg gcgtcgacgt
      721 ggctgcaatg cccgccgccg tttgggccga acatgcggcc ggagggcgac ggttcgccgg
      781 agaatccggg aatcctgtgg tggctggagc atgtgaaagc gcgacacggc gcaggaacgg
      841 cgatgcgcgc gagttggccg tttttcgcga tggataacgg ggtggggcgt ggcgtttgga
      901 cgccggcgca gcaggcgtcg acgttgcgcg aactcggttt cgacggcatc agctacaatt
      961 acacgaacgt cgcggagttg agggtgtggc tgtctgagtt acggcggcag gaactgaaac
     1021 tttacgcgct gtattttccg gtggcgttgc aggggacggc gccgctgccg gacggtttcg
     1081 ccgaagcagt ggatttgctg cgaggctcgg ggaccgcgtt gtggatgttg attccggcca
     1141 agtcacggcc tgccggatac gaggcgatgg cggtcgaacg cgtgcgtgag gtcgcagacc
     1201 tggcggcgga cgcagggttg cgggtcgtgt tgtatccaca tgttcaatgc gtgccggcga
     1261 ccgcggaaga ggcgttttcg ctgagcgagc aggcggagcg agcgaacgtt gcgttgacca
     1321 tgaatctcag ccacgagctc gcagcgggga atggcgcgcg gttgacggag atcgtgcggc
     1381 acgtggtgcc gcgtctcgcc atggtttcga tcaatggcgc gacggacgag ccggggccgc
     1441 tctggaagaa ttacatccga gtgctcggcg agggcgacta cgacgtcgcc gggttgctgc
     1501 gaacgttgcg tgacgtggat tacgggggac cggtgggtct gcagtcgtat ggcatcgaag
     1561 gcaagccggg cgacaacctc gcggcgtcga tgcgcgcatg gaaggcgttg acggaggaaa
     1621 gctcgcgtga aaggcaggag cggcgtcggc cgaaaactta aatttcaaac catggaaacc
     1681 gccccacgaa tcactcgacg ctcgttcgtt aaaaccgggc ttactggaat gctggctgcg
     1741 agggtcgcgc cgcagatcgt gtcctcgcgt ttgttcggcg cgacggcgcc gtcgaatcgc
     1801 gtgacgctcg gctgcatcgg gctcggggtt cacggctttg gcgtgaatct gaaatcgttt
     1861 ctctaccagg atgattgcgt ggtgcgcgcg gtgtgcgacg tgtttggcga gcggcgcgag
     1921 caaggacgca cggcggtcga cgagcgttac ggcaatcgcg attgcgcggt gttcgaggat
     1981 tttcgcgagc tgcttgcgcg gcctgatatc gatgcggtcg tgatctcgac gccggatcac
     2041 tggcacgtcg cgatgtcgct gctcgcgctc gaggcgggca aaatggtttt ctgcgaaaag
     2101 ccgacgctga cgattgcgca ggggcggcga ctcgcggacg aggtgaagcg gcgcgggcta
     2161 ttgttcgcaa cggggctcga ggatcgcgcg gagttgaagt atcacctgct ggcggaagcg
     2221 gtgcgtaacg gtgcgatcgg cacgctgcag cggattcaga tcggacttcc ggaaaaaccg
     2281 gtgttcgcga aagaagagcc ggcgccggtg ccggcggatt tgaattacga attgtggctc
     2341 gggccggcgc gctttcggcc gtatacgccg aacttgacgg acgcgcaggt gtggcggcag
     2401 atcagggatt tctccggtgg gtcgttgacc gactggggtg cgcatctgat cgacacggcg
     2461 caggtggcga atttttccga agacacgagt ccggtcgcgg tcgaaggtgc cggcgagatt
     2521 ccggcc
//
LOCUS       DASPLT010000619         1481 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_171940, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000619 DASPLT010000000
VERSION     DASPLT010000619.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1481)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1481)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1481
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_171940"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            321..611
                     /locus_tag="VEA63_06425"
     CDS             321..611
                     /locus_tag="VEA63_06425"
                     /inference="COORDINATES: protein motif:HMM:NF024363.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH/PUA domain-containing protein"
                     /protein_id="HYD83666.1"
                     /translation="MSAEHELKTWPDFFQAIERGEKTFEVRKNDRGFQKGDVLRLREW
                     DPRKRTVSLSGPGDYTGNAFSVRVTYVLHGEAFGVMDGFAVLGIKRMKGPKP"
     gene            608..1054
                     /gene="dut"
                     /locus_tag="VEA63_06430"
     CDS             608..1054
                     /gene="dut"
                     /locus_tag="VEA63_06430"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF001862.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HYD83667.1"
                     /translation="MTALSLAIEVPCRIKLIDERAQVPAYAHASDTGADLFAVEHTAI
                     MPGETRAVDIGIAIELAPGYCAEIRPRSSQSKRGILVHTGTLDWSYRGSIRVLITNGS
                     EDIYRISPGDRIGQMVVAPVHQARFSVVSELGETARADGGLGSTGV"
     gene            1051..1326
                     /locus_tag="VEA63_06435"
     CDS             1051..1326
                     /locus_tag="VEA63_06435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD83668.1"
                     /translation="MKVSQSILDTALSQPILDTALSQPILATAPYADEVADAVAAIKS
                     SPRLGHLHAIIRRELANAKDGVHANPARWSAAVEMWERLAELFGVRT"
BASE COUNT          238 a          521 c          453 g          269 t
ORIGIN      
        1 actcatgcgc ttggacgtgc cgctcacgac cgcgtcgtac atcgcgactc agcagggcat
       61 gcgcccaacg cctgagcccg ggccgtttga cgatcgcatg ctgcagtggg cgaccgacgt
      121 actcggagaa tcgccatgac cagcccccat cttacggccc accgatcccc cctatcggca
      181 ggccccgccc actcatcccg tgccccgcat cgcgggatgg gtgggcgtct ttctctggcc
      241 gatgccggca tcgcgctcgc gtgctcgctc ggtctcatcc tggttctcga tctttggtcg
      301 gttttggtgg aggtggtgag gtgagcgcag agcatgaact gaagacctgg cctgacttct
      361 tccaggccat cgagcgcggt gagaagacct ttgaggttcg caagaacgat cgcggtttcc
      421 agaagggcga tgtgcttcgg ctgcgcgagt gggacccgcg caagcgcacc gtgtctctga
      481 gcggacccgg tgactacacc ggcaacgctt tcagcgtccg cgtgacgtac gtgctccacg
      541 gcgaggcgtt cggcgtcatg gacggtttcg ctgtgctcgg catcaagcgc atgaagggtc
      601 cgaagccatg accgctctct ccctcgccat cgaagtcccc tgccgcatca agctcatcga
      661 cgagcgcgcg caggtccctg cctacgctca cgcctcggac acgggcgccg acctgttcgc
      721 cgtcgagcac accgctatca tgcccggcga gacccgcgcc gtggacatcg gcatcgcaat
      781 cgagctcgcg cccggctact gcgccgagat ccgcccgcgt tcgtcgcaga gcaagcgcgg
      841 catcctcgtt cacactggca cgctggattg gtcgtaccga gggtccattc gcgtgctcat
      901 cacgaacggc agcgaggaca tctaccgcat ctcgcccggt gaccgtatcg ggcagatggt
      961 ggtcgcaccg gttcatcagg cgcggttcag cgttgttagc gagttgggcg agactgcgcg
     1021 ggccgatggc ggcttgggga gcacgggcgt atgaaggtct cccaatccat cctcgacacc
     1081 gccctctccc aacccatcct cgacaccgcc ctctcccaac ccatcctcgc caccgccccc
     1141 tacgcagacg aggtcgccga cgctgtcgcc gcgatcaagt cctcccctcg cctcggccac
     1201 ctccacgcca tcatccgccg cgagctcgcc aacgcgaagg acggtgtgca tgcgaatccg
     1261 gcgcgctggt cggcggcggt tgagatgtgg gaacggctgg ctgagctgtt tggggtgcgg
     1321 acatgactgt tcgccgctta ggcatggcaa acctcgccat gattaccgcg ctcatggccg
     1381 cgctcatggc ctgcaacgat ctcggtcttg agctgcctga gatggagcct tcggacccgc
     1441 ctccaaggcc cgagcgcgac ccgcagccag ctgtcgatcc g
//
LOCUS       DASPLT010000620         1674 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_273228, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000620 DASPLT010000000
VERSION     DASPLT010000620.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1674)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1674)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1674
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_273228"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1674
                     /locus_tag="VEA63_06440"
     CDS             <1..>1674
                     /locus_tag="VEA63_06440"
                     /inference="COORDINATES: protein motif:HMM:NF014215.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HYD83669.1"
                     /translation="AASPQLSRARLSPDGSHLASLIENDGDTWLVVMNLKTQKALRIS
                     PGKTINGLSKEVGSFRWLSDDRISFLTTVWDGSMWTGVSAVDSNGKRWAAFAGPDVDP
                     RDLKPLVARQIIHSFEDAGQNVLMIDLGSHHGADLVFPDIVKVSTLTGRSDRVVKNPG
                     NVVHWVVDHNGVVRLGVTREGLRFGVIYRENEQAPWRTLPLFDEARGKVTPLGFDYGD
                     QRLIVAATNEQKRRAVYYYDPTTGKLGDMIAGHESYDIVPDRGAASLDGITLAGPVGS
                     DLVGTVVGIRYFTEGPRVRWFDPGLEILQKAIDNRLPGTVNLIVSRSRDEKRFLVLAF
                     SDRDPGTYYLVDLASEKPGFSRIGQPMPNFPVDRMAAMLPIKYQARDGETIHGYLTLP
                     SGDRKKNLPLVVMPHGGPTVRDIWAYNPTVQFLASRGYAVLQVNFRGSPGYGSEFYEK
                     GKREIGRGIQTDIDDGTRWAIAHGFADPARVAIVGSSYGGYSALFALAHSPELYRCGI
                     SINGVTDWTDIVRERKGEEYRYAYLHFQEWIGDPKTDANFLASISPVTFA"
BASE COUNT          318 a          579 c          476 g          301 t
ORIGIN      
        1 gccgccagtc cccagctctc gcgagcgcgc ctctctcccg acggaagcca tctcgcctcg
       61 ctcatcgaaa acgacggcga cacctggctg gtcgtcatga atctcaagac gcaaaaggcg
      121 ctgcgcatca gtcccggtaa aacgatcaac ggcttgagca aggaggtcgg ctccttccgc
      181 tggctgagcg acgaccgtat cagctttctc acgacggtgt gggatggctc gatgtggacc
      241 ggcgtcagcg ccgtcgattc caacggcaag cgctgggcgg ccttcgccgg ccccgatgtc
      301 gacccgcgcg acctcaagcc gctggtcgct cgccaaatca tccacagttt cgaagacgcc
      361 ggccaaaacg tgctgatgat cgatcttgga tcgcatcacg gcgcggacct cgttttcccc
      421 gacatcgtca aagtcagcac gctcaccggc cgctccgatc gcgtcgtcaa aaatcctggc
      481 aacgtcgtcc actgggtggt ggatcacaac ggcgtggtgc ggctcggcgt cacgcgcgag
      541 ggcctgcgct tcggtgtcat ttatcgcgag aacgaacaag ccccttggcg cacgctcccg
      601 ctcttcgatg aagcgcgcgg caaggtcact ccactcggct tcgactatgg agatcaacga
      661 ctgatcgtgg ccgcgacgaa cgagcaaaaa cggcgcgctg tctactatta cgatccgacc
      721 accggaaagc tcggggacat gatcgcgggc catgagagct acgacatcgt ccctgatcgc
      781 ggcgccgcct cgctcgatgg catcacgctc gccggccccg tgggctccga tctcgtcggc
      841 accgtcgtcg gcatccgcta cttcactgaa ggcccgcgcg tgcgctggtt cgatcccgga
      901 ctcgagatcc tccagaaggc gatcgataac cgccttccgg gcaccgtgaa cctgatcgtc
      961 agccgctctc gcgacgaaaa acggtttctc gttctcgcgt tttccgatcg cgatcccggc
     1021 acctactatc tcgtcgacct cgcctccgaa aaacccgggt tctcgcgcat cggccagccc
     1081 atgccgaatt ttcccgtcga ccgcatggcg gccatgctgc cgatcaaata tcaagcacgc
     1141 gacggcgaga ccattcacgg ttacctgacc ctcccctcgg gtgatcggaa gaaaaacctc
     1201 ccgctggtcg tcatgcctca cggcgggccg acggttcgcg acatctgggc ctacaacccc
     1261 acggtgcaat ttctcgccag ccgcggatac gcggtccttc aggtgaactt ccgcgggtcc
     1321 cccggctacg gcagcgagtt ctacgagaaa ggaaaacgcg agatcggtcg aggaatccaa
     1381 actgacatcg acgacggcac gcgctgggcc atcgcgcacg gcttcgcgga cccggcgcgc
     1441 gtcgcgattg tcggcagcag ctacggcggc tactccgcgc tattcgcgtt ggcccacagc
     1501 cccgagcttt accggtgcgg catctccatc aacggcgtca ccgactggac cgacatcgtg
     1561 cgcgaacgaa agggcgaaga gtatcgttac gcctatcttc acttccagga gtggatcggc
     1621 gatcccaaga cggacgccaa cttcctcgcc tcgatttcac ccgtgacgtt cgcg
//
LOCUS       DASPLT010000621         1014 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_253346, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000621 DASPLT010000000
VERSION     DASPLT010000621.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1014)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1014)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1014
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_253346"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1014
                     /locus_tag="VEA63_06445"
     CDS             <1..>1014
                     /locus_tag="VEA63_06445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007360154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HYD83670.1"
                     /translation="YRLDFFGDEIEEIRAFDPVTQRSGATVESITISASSRVKLDPAK
                     TGIADYLTPRTHLVFVEPASLDETFSAHAREGADQLTPLLEKCAAAFGVSDLDEASAL
                     FEDAASEITWDSESLGHHRHYPDDALVAQERLQVEEEARAKFLLQIAAWKKEGYRPLF
                     VVTKDGEEQRIREILGEDARFKSIQPRFLRGGLNEGFRVTFRENAALSLSAPLTRSKT
                     KADSARGLVVVTETEIFGRQRQRRASLNTRAIAQRAQIDQLLDFAELVEGDFVVHLQH
                     GIALYRGLTKLDTAQGMREVISLEFDDHVTLHVPLQESHLISRYVGLSKTKPQLGRIG
                     SNR"
BASE COUNT          211 a          342 c          278 g          183 t
ORIGIN      
        1 cgtatcggct cgatttcttc ggcgacgaaa tcgaagagat tcgcgcgttc gatcccgtca
       61 ctcagcgctc gggcgcgacc gtcgagagca tcacgatttc cgcgtcgtct cgcgtgaaac
      121 tcgatccggc gaaaaccggc attgcggatt atctcacgcc gcgcacgcac ctcgttttcg
      181 ttgaaccggc gtcactcgac gaaaccttca gcgctcacgc ccgcgaaggc gcggatcaac
      241 tcacgccttt gctcgaaaaa tgcgccgctg cgttcggcgt cagcgatctc gacgaagcca
      301 gcgcgttgtt cgaagacgcc gcttcggaaa tcacctggga ctccgagagc ctcggacatc
      361 atcgccacta ccccgacgac gcgctcgtcg cccaggagcg cctgcaagtc gaagaagaag
      421 cgcgcgcgaa attcctcctg caaatcgccg cgtggaaaaa ggaaggttac cgcccgttgt
      481 tcgtcgtcac caaagacggc gaggaacagc gcatccgcga aatcctcggc gaagatgcac
      541 gcttcaaaag catccagccg cgcttccttc gcggcggact caacgaaggc ttccgcgtca
      601 cgtttcgcga aaatgccgca ctgtcgcttt ccgcgccact gacgagatcg aaaacgaaag
      661 ccgacagcgc ccgcggcctc gtcgtcgtca ccgaaacgga gatcttcggc cggcaacgcc
      721 agcgccgcgc ttccctcaac acccgcgcaa tcgcccagcg cgcacagatc gatcagttgc
      781 ttgatttcgc cgagttggtc gaaggcgatt tcgtcgtcca cctccagcac ggcatcgcgc
      841 tttatcgcgg cctgaccaag ttggatactg cgcagggcat gcgggaggtg atctcgctcg
      901 aattcgacga tcacgtcacg ctgcacgtcc cgctccagga atcgcatctc atcagtcgtt
      961 atgtcggctt gagcaaaacg aagccgcagc tcgggcgcat cggctccaat cgct
//
LOCUS       DASPLT010000622         2660 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_130626, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000622 DASPLT010000000
VERSION     DASPLT010000622.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 2660)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2660)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2660
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_130626"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..628)
                     /locus_tag="VEA63_06450"
     CDS             complement(<1..628)
                     /locus_tag="VEA63_06450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD83671.1"
                     /translation="MLVAFFADRGHAEVTPYPRVPGDEANSDYVSVRVNGVEVDTVGT
                     SMNVGYAHFAFAGTAKVEIAAKEPIETFDLSPHRLKIPASVAGNVLTLQLTEPRKLHL
                     RVNGLSRFFIFAEAPERNAPQPGQPGVISVGEFGVVSDPQKTQTEALQRAIDQVAETR
                     GVLHVPPGIYRTGRLLLRSNLSLYLAPGAIIKGTGKLADYPRTDGNTQQ"
     gene            complement(697..>2660)
                     /locus_tag="VEA63_06455"
     CDS             complement(697..>2660)
                     /locus_tag="VEA63_06455"
                     /inference="COORDINATES: protein motif:HMM:NF024777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LamG-like jellyroll fold domain-containing
                     protein"
                     /protein_id="HYD83672.1"
                     /translation="PGSGNPSIQATDTPGVFTHRVTRTNDSIEPGGKWLIKNKRGGVV
                     YLTTRAGAENITLSGVDGRASGGGHLRFWQTSGVNILNCRFEPERNNWISSSADGVHG
                     RGREGVWIEDTLIRGVCEDIMNTYGQNMIVEPDDDNEDAVMSIRMFTRSSESFNDGAP
                     RLPTEESVAVGDQLVFFDPRTGRVQGYAGVVSIANGRYTLTNPVPGIDPWHETDGKNA
                     TMVYNTHVAGRFFVRDTRMTDSMRFGIYIKARGGVVFGSQFEGLSAPPIFAVNEPEWP
                     EGPPATHLWVQGCTFSQNNYGYMPRNRDFMVVDPADISVYTRRFRAPSEPDDYRANLT
                     RGQYANSHMKFIGNVFHDWRGMGISVRNSRNARIEENVFLSPVNDETMRKTLASDPVM
                     SVGGHGGYAAIFLDSVGGVRVSGNWFHGLPAGDRGLVKGEDVEGLIADNNVDSASTSS
                     TVSLSFDEWFGDTSIARNAGGEPVGMLALGGGVHRAGRLGGGVFFAGGAPATFDELSD
                     VGGVRLGRFGLALWVCAEEAKATGTVFVLGSERDGIVIALKNGRWRASLRRGGRGGWL
                     ELGAATAGHWQHLALIFDGEARIMHGYTDGVELTRQAGVATRIDLPAKGEVGGGTFRG
                     GVDEVRWTTAKWSAADVAALALRKPREGL"
BASE COUNT          470 a          962 c          735 g          493 t
ORIGIN      
        1 actgctgcgt gtttccatcc gtgcgcggat agtccgcgag cttgcccgtg cctttgatga
       61 tcgcgccggg cgcgagatac agcgagagat tgctccgcag cagcaggcgt cccgtccgat
      121 aaatccccgg cggaacgtgc aacacaccac gcgtctccgc cacctgatcg atcgcgcgtt
      181 gcaacgcctc cgtctgcgtc ttctgcggat cgctcacgac gccgaattcg ccaacactga
      241 tcacccccgg ttggccgggc tgcggcgcat tcctctcggg cgcctccgcg aaaataaaga
      301 agcggctcag cccgttgacg cgcaggtgca gctttcgcgg ttcggtgagt tgaagggtca
      361 acacattgcc cgcaacgctc gccgggatct tcaaccgatg cgggctgagg tcgaacgtct
      421 cgatcggctc cttcgccgca atctccacct tcgccgttcc cgcgaacgcg aaatgcgcgt
      481 atccaacgtt catggacgtg ccgacggtgt cgacctccac tccattcacg cgcacggaaa
      541 cataatccga gttcgcttca tcgcccggca cgcgcggata cggcgtgacc tcggcgtgac
      601 cacgatccgc gaaaaacgct accaacacgg cggcgatcaa cccacgaaga agcgcgccgc
      661 ttaggccgac tctgccgagc ctgtcggata cgctcatcat aaaccttccc gcggtttacg
      721 cagcgcgagc gcggcgacat ccgcggcgct ccatttcgcg gtcgtccatc gcacttcgtc
      781 aacgccgccg cggaacgttc cgccgcccac ctcgcctttc gccggcaagt cgatccgcgt
      841 cgccacaccc gcctgtctcg tgagctccac gccgtcggtg taaccgtgca tgatccttgc
      901 ttcaccgtcg aagatgagcg cgagatgctg ccagtgaccg gcggttgctg cgccaagctc
      961 gagccacccg ccgcggccgc cgcgtcgcaa cgacgcgcgc caacggccgt tcttgagcgc
     1021 gatcacgatt ccatcccgct cgctgccgag aacgaagacc gttcccgtcg ccttcgcctc
     1081 ttccgcacac acccaaagcg ccagtccgaa acggcccagg cgcaccccgc cgacgtcgct
     1141 caactcgtcg aatgtcgccg gtgcgccgcc cgcgaaaaac acgccgccgc cgagccgtcc
     1201 agcccgatgg acgccgccgc caagcgcgag cattccaaca ggctcgccgc ctgcgttccg
     1261 cgcgatgctc gtgtcgccga accactcgtc gaacgaaagc gacaccgtcg atgacgtcga
     1321 tgcgctgtcg acattgttgt ccgcgatcaa tccttcgacg tcctcacctt tcaccagccc
     1381 acgatcgcct gccggcaacc cgtgaaacca gtttccagat acacgcactc cgccgacgct
     1441 atcgagaaag atcgcggcat atccgccatg tccgcccacg ctcatcaccg gatcgctcgc
     1501 cagcgtcttc cgcatcgtct cgtcgttcac cggcgaaagg aaaacatttt cctcgatgcg
     1561 cgcgttgcgc gagttgcgca ccgaaatccc catcccgcgc cagtcgtgaa atacatttcc
     1621 aataaatttc atgtgactgt tcgcatactg cccgcgcgtg aggttcgccc ggtaatcgtc
     1681 gggctccgac ggcgcgcgga agcgccgcgt gtaaacgctg atgtccgccg gatcgacgac
     1741 catgaaatcg cggttgcgcg gcatgtagcc gtagttgttt tggctgaagg tgcagccctg
     1801 cacccacaag tgcgtcgcgg gcgggccctc gggccattcg ggctcgttca ccgcgaagat
     1861 cggcggcgcg gaaagccctt cgaactggct gccgaacacg actccaccgc gcgccttgat
     1921 gtagatgccg aaacgcatgc tatccgtcat ccgggtgtcg cggacaaaaa accgcccggc
     1981 gacatgcgtg ttataaacca tcgtcgcatt cttcccgtcg gtctcgtgcc acggatcgat
     2041 gcccggcacc ggattcgtga gcgtgtaacg cccgttcgcg atcgacacca cgccggcata
     2101 gccttgcacc cggccggtgc gcggatcgaa aaacacgagt tggtcgccca ccgccacact
     2161 ctcctccgtc ggcaaacgcg gcgcaccgtc gttgaacgat tcgctgctgc gcgtgaacat
     2221 ccggatcgac atcaccgcgt cttcgttgtc gtcgtccggc tcgacgatca tgttctgccc
     2281 gtaggtgttc atgatgtcct cgcagacgcc gcggatcaac gtgtcttcga tccacacgcc
     2341 ttcgcgtccg cgtccgtgca ccccgtcggc gctcgagctg atccagttgt tcctttccgg
     2401 ttcgaagcgg cagttcaaga tgttgacgcc cgacgtctgc cagaatcgca ggtgcccgcc
     2461 gcccgacgcg cgaccgtcga ctcctgacag cgtgatgttc tccgcccccg cgcgcgtcgt
     2521 cagataaacg acgccgccgc gcttgttttt tatcaaccac ttcccgcccg gctcgatcga
     2581 atcgttggtg cgcgtcacgc gatgcgtgaa cacccccggt gtatccgtcg cctggatcga
     2641 cggattcccg ctgcccggat
//
LOCUS       DASPLT010000623         1302 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_212538, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000623 DASPLT010000000
VERSION     DASPLT010000623.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1302)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1302)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1302
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_212538"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1086)
                     /locus_tag="VEA63_06460"
     CDS             complement(<1..1086)
                     /locus_tag="VEA63_06460"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HYD83673.1"
                     /translation="MVRAFKPTANAAAMLSWRGRLWFAFGMALIVVAGCRRAETSLDL
                     PARPGAEVSALLRARLDDAEAQVRAAPGAAERWRRLAAVYHANGFAGEAIACYRHAIE
                     CAGDNATTGELHYLLAVAAEEAGDVETAETALRETLKRIPNAVPALLRAGDLRYKAGD
                     ADAAMVFYRAASAIDVNNPEALLALAREALRKEDDAIALELLDRLERAHPGHGSGLSL
                     KAQLMERRGEYSQADAIRRQRRVRKDRPMRDPLLDEVMLACVDVPRLGLRFEDELNAG
                     RIDDALATLDRMQAIEPGNWLTYRLRGFALAHSGRPEAAVEAYRKAIEFGGDPAVVYP
                     GLVSVLVKLERFDEAAQFAREGLAKAPG"
BASE COUNT          219 a          478 c          395 g          210 t
ORIGIN      
        1 cccgggagct ttcgcgaggc cttcgcgcgc gaattgcgcg gcctcgtcga aacgttcgag
       61 tttcacgaga acagagacca gccccggata aaccaccgca gggtcgccac cgaattcgat
      121 cgccttgcga tacgcctcaa ccgcggcttc gggccgtccg ctgtgcgcga gcgcgaaccc
      181 acgaagtcga tacgtcagcc agttgccggg ctcgattgcc tgcatgcggt cgagcgtcgc
      241 cagcgcgtcg tcgatccgtc ccgcgttcaa ctcatcctca aaacgcagcc cgagtcgtgg
      301 cacgtcgacg catgccagca tgacttcgtc gagcaagggg tctcgcatcg gccggtcctt
      361 gcgcacgcgg cgttggcgcc gaatcgcgtc ggcttgggag tattcaccgc gccgctccat
      421 caactgggct ttgagcgaca agccggagcc gtggcccggg tgcgcacgtt caaggcggtc
      481 gagcagctcc aacgcaatcg cgtcatcctc tttccgcagc gcctcgcgcg cgagcgccag
      541 cagcgcctcg gggttgttca cgtcgatggc cgacgccgcg cgataaaaaa ccatcgccgc
      601 gtctgcatcg ccggccttgt agcgcaggtc gcccgcgcgc agcagcgccg gcactgcatt
      661 cgggatgcgc ttcaacgttt cgcgcaacgc ggtctccgcc gtttcaacgt cgcccgcttc
      721 ctccgccgcg accgcgagca gataatgcag ttcgccggtc gtcgcattgt cacccgcgca
      781 ttcgatcgca tgccgataac aggcgatcgc ttctccagcg aagccgttcg catgataaac
      841 cgccgcgagc cggcgccagc gctccgcggc acccggcgca gctcggacct gcgcctccgc
      901 atcgtcgagt cgcgcgcgga gtagcgcgga aacttccgcg ccgggccgcg ccggcagatc
      961 gagcgaggtt tcggcccgcc gacatcccgc cacgacgata agcgccatcc cgaacgcgaa
     1021 ccacagccgc ccacgccagc tcagcatcgc agcggcattc gctgtgggtt tgaacgcacg
     1081 gaccatcacg atcgcagggg taacggaacc atcatcgagc ggttggccga actgaccacc
     1141 gaccgccggg agcttgaatc gaagggaccg cgttgcgaac gggaaagcat cgcgaatctc
     1201 caccgtagcg gcaggcctcc gtgcctgcct gtccgcttgc cggatgcctc tgcctgttgc
     1261 ctgccgcaac ccccgcgcgc ccttcgcccg ctcttcgcca gc
//
LOCUS       DASPLT010000624         1527 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_294666, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000624 DASPLT010000000
VERSION     DASPLT010000624.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1527)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1527)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1527
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_294666"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..233)
                     /locus_tag="VEA63_06465"
     CDS             complement(<1..233)
                     /locus_tag="VEA63_06465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009514036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HYD83674.1"
                     /translation="MNTTKKILLFAAAIAAAFVTAVQADVKVGAPAPDFSVTDIQGKA
                     HRLSDYKGKTVVLEWVNPECPIVMKHYGSGNMP"
     gene            438..>1527
                     /locus_tag="VEA63_06470"
     CDS             438..>1527
                     /locus_tag="VEA63_06470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD83675.1"
                     /translation="MIGWLADFFRFAWGLLYWNTRKTWFQLRRGRSPCPCQSPSDSGR
                     AYETGCDACLSWDKSARFRRVCPLLVETPDGLRCSANTADVRPFWGRAFGVYGGTAAA
                     LYLGGALAVFIFLRTVGYPINIGHLVWPGAWHRVTEVRGWFFLERAKAAFNEGRTAEG
                     MLYLGNAHQFDPANYTIASMFAQRLQLSHPVRADDIYRRLREDHPAQRALVSETWFRS
                     LLARGEFAAVATLARDEILVDPDRAGVWMRALIFASRQTNSDSALRELLTSAEPAAQP
                     WRQLLETELLLRTGRRTEARDALSRPWENIPAYARFYQISELIALGDGIAAVDFVARY
                     GSTLDDTARVVLLLDAYTTLGAAQSRERL"
BASE COUNT          242 a          589 c          379 g          317 t
ORIGIN      
        1 ttcggcatgt ttccgctgcc gtaatgcttc atcacgatcg ggcattccgg gttcacccat
       61 tccagcacca ccgtcttgcc tttgtaatcc gacaatcgat gcgccttgcc ctgaatgtcc
      121 gtgaccgaaa agtccggcgc cggcgctcca accttcacgt ctgcctgcac cgccgtcacg
      181 aaagcagcag cgatggccgc tgcgaaaaga aggattttct tggtcgtgtt catgggaatc
      241 gagggcggta gacgcagcgc tctggcgaaa gattcgagcg ccgccccaac atgggagttc
      301 tcccgctagc tcgatctcct tctgccccca aatcgccctt tccgatcgca taaaacctct
      361 cgtcggtttc cgtcgcgccc tcctccgtcg ccgttttcct ttacgcttct gacgtcgccg
      421 cctagggtct ctcccctgtg attggctggc tcgccgactt cttccgcttc gcttggggac
      481 tgctttattg gaacacccgc aaaacctggt tccagcttcg ccgcggacgc tcgccctgcc
      541 cctgccaaag cccaagcgat tccggccgtg cttacgaaac cggttgcgac gcctgccttt
      601 cctgggacaa atccgcgcgt ttccgtcgcg tttgcccctt gctcgtcgaa acccccgacg
      661 gccttcgctg ctccgcgaac accgctgacg tccgtccttt ctggggccgc gcgttcggtg
      721 tctacggcgg cacagcagcc gcgctctacc tcggcggagc actcgcggtc ttcatttttc
      781 tccgcaccgt cggctacccg atcaacatcg gccatctcgt ctggcccggt gcctggcacc
      841 gcgtcaccga agtgcgcggc tggttcttcc tggaacgcgc caaagccgcc ttcaacgaag
      901 gccgcaccgc cgaaggcatg ctctacctcg gcaatgcgca ccagttcgat cctgccaact
      961 acaccattgc gtcgatgttc gcgcagcggc tccaactcag tcacccggtg cgtgccgacg
     1021 acatctaccg ccgcctccgc gaagatcatc ccgctcaacg cgccctcgtc agcgaaacct
     1081 ggtttcgttc gctcctggct cgcggcgaat ttgccgccgt cgcaacgctc gcccgcgacg
     1141 aaatcctcgt cgatcccgac cgcgccggcg tctggatgcg cgccctcatc ttcgcctccc
     1201 gccaaaccaa ttccgactcc gcgctccgcg aacttctcac ttccgccgaa cccgccgcgc
     1261 aaccctggcg tcaacttctc gaaaccgaac tcctccttcg caccggtcgc cgcaccgaag
     1321 ctcgcgacgc ccttagtcgc ccttgggaaa acatccccgc ctacgcccgc ttttatcaaa
     1381 tcagcgaact catcgcactc ggcgacggca tcgccgccgt ggactttgtc gcccgctacg
     1441 gttcgaccct cgacgacacc gcgcgcgtcg tcctcttgct cgacgcctac acgacactcg
     1501 gcgccgccca atcccgcgaa cgtctcg
//
LOCUS       DASPLT010000625         1207 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_212578, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000625 DASPLT010000000
VERSION     DASPLT010000625.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1207)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1207)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1207
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_212578"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(12..836)
                     /locus_tag="VEA63_06475"
     CDS             complement(12..836)
                     /locus_tag="VEA63_06475"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HYD83676.1"
                     /translation="MLDYLERKFGRIALQNVTLYLIIGQVFVTLTWMLGLLDQNRLVL
                     VPYLVLHGEPWRLITFLFLPPPVSGTFGLMLLPFAWWLFYLMGNALEHYWGAFRYNIF
                     LLLGIALTVGAAFLSPMSPASNLFLAGTVFLAFAWLNPDFEIMLFFILPVKIKWLAAI
                     TAVGYAVQLVVGPATVRWQVLASIGNFLVFFGRDIVNAIRYRQRSIATHAKRVAEERK
                     GPEARHRCHICGKTDLTNPEMDFRYCSKCAGDQCYCPEHIFNHEHVTAEPENGEKA"
     gene            complement(876..>1207)
                     /locus_tag="VEA63_06480"
     CDS             complement(876..>1207)
                     /locus_tag="VEA63_06480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD83677.1"
                     /translation="DAINRSIHEAFKKAAELAPDRIEFTYRYAESFYHLDPPDWDAAL
                     KAWGALEEKAPTPVERETMRLHAANVLILQGKREHAQALLDTVTEEALQKQKEKLVAQ
                     LSEPVEK"
BASE COUNT          249 a          379 c          363 g          216 t
ORIGIN      
        1 gggaaacgtc gctacgcctt ctcgccgttt tccggttcag cggtgacgtg ctcgtggttg
       61 aagatgtgct ccgggcagta gcattggtcg ccggcgcact tcgaacagta gcgaaagtcc
      121 atctcgggat tcgtcaggtc ggtcttcccg caaatgtgac agcgatgccg cgcctccggg
      181 cctttgcgtt cttctgcgac gcgtttcgcg tgcgtcgcga tcgagcgctg acgatagcgg
      241 atcgcgttca cgatatcgcg gccgaagaac accaggaagt tgccgatcga cgcgagcacc
      301 tgccacctca ccgtcgccgg cccgacgacg agctgcaccg catagccgac cgccgtgatc
      361 gccgcgagcc acttgatctt caccggaagg atgaagaaca gcatgatctc gaaatccgga
      421 ttcaaccacg cgaacgccag gaacaccgtg ccggcgagaa acagattcga cgccggcgac
      481 atcggcgaaa gaaaggccgc tccgaccgtc agcgcgatgc cgagcagcag gaaaatgttg
      541 tagcgaaacg cgccccagta gtgctcgagc gcgttgccca tcaggtaaaa caaccaccac
      601 gcgaacggca gcagcatgag cccgaacgtg ccgctcaccg gtggcggcaa aaagagaaac
      661 gtgatcagcc gccacggttc gccgtggagc accagataag gcacgagcac cagccggttc
      721 tgatcgagca ggcccagcat ccaggtcaac gtcacgaaga cctgtccgat gatcaggtag
      781 agcgtgacat tctggagcgc gatgcggcca aacttgcgtt cgagatagtc gagcatgaga
      841 gaaacgggac ggccgcgggg aaattgcgcg accgcctact tctcgacggg ctccgacaac
      901 tgcgcaacga gcttttcctt ctgtttttgc agcgcctctt ccgtgacggt gtcgagcagc
      961 gcctgcgcgt gttcgcgttt gccttgcaaa atcaacacgt tcgccgcgtg caagcgcatc
     1021 gtctcgcgct ccaccggcgt cggcgctttt tcctcgagcg cgccccacgc tttcaacgcc
     1081 gcgtcccagt ccggcggatc gagatggtaa aacgattccg cgtagcgata cgtgaactcg
     1141 atccggtccg gcgcgagctc cgccgctttc ttgaaggctt cgtggatcga gcggttgatc
     1201 gcatcgg
//
LOCUS       DASPLT010000626         1800 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_294692, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000626 DASPLT010000000
VERSION     DASPLT010000626.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1800)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1800)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1800
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_294692"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..563
                     /locus_tag="VEA63_06485"
     CDS             <1..563
                     /locus_tag="VEA63_06485"
                     /inference="COORDINATES: protein motif:HMM:NF027666.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sulfatase/phosphatase domain-containing protein"
                     /protein_id="HYD83678.1"
                     /translation="DSTVIVFASDHGYHLGEHGLWQKRSLFEESARVPLLVVAPGVSK
                     ANTTVAAPVSLIDLFPTLAELCGINAPANLQGQSLVPMLRDPSVLGRGWAITQTAHNG
                     VSGYSLRTPRWRYTEWEGAPGRELYDHDTDPSELTNLATDPAHAKTIEALSAQLRAAV
                     SATFPPGGERPAVREGALWHPVLVTP"
     gene            complement(795..>1800)
                     /locus_tag="VEA63_06490"
     CDS             complement(795..>1800)
                     /locus_tag="VEA63_06490"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HYD83679.1"
                     /translation="SCDPYRLEVLGAMRMQVGQLQQSLPFYEKAVELAPRDGPARDAL
                     AEVYLQLGRLEDARAALDAGIAATPDYPPLYSTMAGLLRRQSKHAEAIAILQRGLRAL
                     PESPELHNDIGISLEAAGRQAEAIDAYREATRLNPNFPEAHLNLGLCLLAAGDTHAAQ
                     QSLDRTLRLRPADLKTLVALARKAFEEERLDAAEGYVRTLQEFRPGLPATRQLTGLLH
                     LRRGNRAAAAGRIDDAAAEFRAGLVADPESPDLHANLGVLHARAGQHEAAIAEFQLAL
                     NADPANGRLLLYLGQVLLQAGRAPEAREVLVRGLASARAAGQAELQAQISQVLGSLGR
                     "
BASE COUNT          315 a          642 c          540 g          303 t
ORIGIN      
        1 cggacagcac ggtgatcgtt tttgcgagcg accatggtta tcatttggga gaacatggac
       61 tctggcagaa acgaagtttg ttcgaggaaa gcgcgcgcgt gccgctgttg gtggtggcgc
      121 ccggcgtgtc gaaagcgaac acgaccgtag ccgctccggt gagcttgatc gacctgttcc
      181 ccacgctggc cgaactctgc ggaatcaacg cgccggccaa tctccagggg cagagccttg
      241 tgccgatgtt gcgcgatcct tctgtgcttg ggcgtggttg ggcgatcacg cagacggcgc
      301 acaatggtgt ttccggctac agcttgcgca cgccgcgctg gcgctacacc gagtgggaag
      361 gcgcgccggg ccgcgaactc tacgatcacg acacggatcc gagtgaactc accaatctcg
      421 cgaccgatcc cgcgcacgcg aaaacgatcg aggctctctc tgcccaacta cgcgccgcgg
      481 tgagcgcaac cttcccgccg ggcggagagc ggcccgcggt gcgcgaaggt gcgttgtggc
      541 acccggtgct ggtcacgccg taattgaggg tcggctttcg tgctgccggc cgcggacgac
      601 acggaggtcg tccctccgga aatggctttg ttgaaggctc gttagccggc gcgcccaggc
      661 tcacgccttc cgccgatggc ttcagattgc gacgcctcct caccgtaatt cgcggtcaag
      721 agtcaccctt gctttgctcc attctctccg aatctcaaac tcagaaccca caaccccaaa
      781 ctccaaaccc agcctcaccg ccccaaactc cccaacacct gcgaaatctg cgcctgtaac
      841 tccgcctgcc cggctgccct cgccgacgcg agtcctcgca ccagcacctc gcgcgcttcg
      901 ggcgcacgtc cggcctgaag caacacctga ccgagataca gcagcagacg gccgttcgcc
      961 ggatccgcgt tcaacgccag ttgaaactcc gcgatcgccg cctcgtgttg ccccgcccgc
     1021 gcgtgcagca cgccgagatt cgcatgcaaa tccggcgact caggatcggc caccaggccc
     1081 gcgcgaaact cggccgccgc atcgtcgatg cgccccgccg ccgccgcgcg attcccccta
     1141 cgcaaatgca gcaagcccgt gagctggcgc gtcgccggca atcccggacg aaactcctgc
     1201 aacgtccgca cgtaaccttc cgccgcgtcc agccgctcct cctcgaaggc ctttcgcgcg
     1261 agcgcgacca gggttttcag atccgcgggc cgaagccgca gggtgcgatc gaggctttgt
     1321 tgcgccgcgt gcgtgtcgcc cgccgccagc aggcaaagcc cgaggttgag atgcgcttcc
     1381 ggaaaattcg gattgagccg cgtcgcttcg cgataggcgt cgatcgcctc ggcctgccgc
     1441 cccgccgcct cgagcgaaat gccgatgtcg ttatgcaatt ccggcgactc cggcagcgcg
     1501 cgcagcccgc gctggagaat cgcgatcgcc tcggcgtgtt tcgactggcg acgcagcaac
     1561 cccgccatcg tcgaatacaa cggcggataa tccggcgttg ccgcgatgcc cgcgtcgagc
     1621 gccgcgcgcg cgtcttcgag acgcccgagc tgtagataaa cttctgcgag cgcgtcccgc
     1681 gccggtccat cgcgcggcgc gagctccacg gctttctcat aaaacggaag cgattgctga
     1741 agctggccga cctgcatgcg catcgcgccg agcacctcga gccgatacgg atcgcaactc
//
LOCUS       DASPLT010000627         1292 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_47048, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000627 DASPLT010000000
VERSION     DASPLT010000627.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1292)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1292)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1292
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_47048"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            206..1108
                     /locus_tag="VEA63_06495"
     CDS             206..1108
                     /locus_tag="VEA63_06495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HYD83680.1"
                     /translation="MSSTALPLRHLLLALLVVAIWGVNFVAIKVALRELPPLLLCAVR
                     FTLVSVPVVLLLPRPAVPWRQLIVYGLTMFALHFGFLFLGMKLGMSAGLASLTLQFQV
                     FVTLALAATVLKERIAPVQIAGALTACAGFGIVAAHTGGEVTLAGLACVLLGATSWGY
                     ANFTSKRLGVINPLALVVWASLVVPVPMALASLIFEGPALIAHSLTHVGGATVFSVAF
                     IVYISTLVAYSLWSGLLSRHPASIVAPFTLLVPIFGMLSSALLLGESLPAWKLHAAAL
                     VIAGLALNLCGPRFLSVRARARSA"
BASE COUNT          191 a          473 c          349 g          279 t
ORIGIN      
        1 cgttgatccc acccgaaacc cgccacaccg cgacgcctcg ttcacgatcc cgaacaacga
       61 acgccacgtc ctcggtgccc gaagccaaag catcccgcga cgcgcgaccc gcacaccgcc
      121 ccacatttgc agccaacacg gccgccaacc tccggttctg ggtttggacg ccgcacagac
      181 tcgctgctta gctcatctct cgcccatgtc ctccaccgcg ctcccgctcc gtcatctgct
      241 gctcgccttg ctcgtcgttg cgatttgggg cgtgaacttc gtcgcgatca aggtcgcact
      301 ccgcgagctg ccgccgctgc tcctgtgcgc cgtccgcttc acgctcgtgt ccgtgccagt
      361 cgtgctcctg ctgccgcgac ccgccgttcc ctggcgacaa ctgatcgttt acggactcac
      421 catgttcgcg ctgcatttcg gctttttgtt tcttggcatg aagctcggca tgtcggccgg
      481 tctcgcttcg ctgacgctcc aattccaagt cttcgtcacg ctcgcgctcg ccgcgaccgt
      541 gctgaaagag cgcatcgcac cggtgcagat cgccggcgcg ctcaccgcgt gcgccggctt
      601 cggcatcgtg gccgcgcaca ccggcggcga agtcaccctc gccggcctcg cctgtgtgct
      661 gctcggcgcc acgtcgtggg gttatgccaa cttcacctcg aaacgcctcg gcgttatcaa
      721 tcccctagca ctcgtcgtgt gggcgagtct ggtcgtgccg gtgccgatgg cgctcgcttc
      781 actcatcttc gagggtcccg cgctgatcgc gcacagtctc acacacgtcg gtggcgcgac
      841 cgtgttcagc gtggcgttca tcgtctacat ttccacgctg gtcgcctatt cgctctggag
      901 tggattgctc agccgccatc ccgcctcgat cgtcgcaccg ttcacgttgc tcgtgccgat
      961 cttcggcatg ctcagttcgg cgctgctgct cggcgaaagc ctgcccgcgt ggaaactcca
     1021 cgccgccgcc ctcgtcatcg ccggactcgc gttgaacctc tgcggcccgc gcttcctctc
     1081 tgtccgcgct cgcgctcgtt cggcttagcg gaatggactc aatcgaacca cgaatgggtc
     1141 ggttgcccgc gaatcacgcg aacggccgcg aatcagaata aggagaaatg atctttcacc
     1201 aaacactgac ctggcgttga aaccgctaga tctggtttca cctctgaaat tcgcgtccat
     1261 tcgcgtcatt cgcgggaggt cttctgcatg aa
//
LOCUS       DASPLT010000628         1044 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_130688, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000628 DASPLT010000000
VERSION     DASPLT010000628.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1044)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1044)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1044
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_130688"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..210)
                     /locus_tag="VEA63_06500"
     CDS             complement(<1..210)
                     /locus_tag="VEA63_06500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HYD83681.1"
                     /translation="MKKHSFVLGAVALAFSLVAPVSVSAGAPPPNVVIIVADDLGVTD
                     LSVLGKRDVLTPHIDSIAQQGTSFTQ"
     gene            complement(221..>1044)
                     /locus_tag="VEA63_06505"
     CDS             complement(221..>1044)
                     /locus_tag="VEA63_06505"
                     /inference="COORDINATES: protein motif:HMM:NF027666.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HYD83682.1"
                     /translation="AVRFIHERKDGPFFLYLSQYYVHTPIRTRTPWLTEKYRAKLPAD
                     ASPDRAVYAGMVETLDHLVGRVLQAIDEAGVAENTLVVFMSDNGGHPEYAANGPLRGS
                     KWNVYEGGIRVPFIVRWPGRVAAGSVSDTPIIGSDLMPTLSAVAGTTPPAGVPMDGVN
                     IMPLLSGAKSVERSQPIVWHFPYYHPEGKFAEAKPDIGIDDFKTSQTRPQSAIRMGNY
                     TLIHFYENDRDELYDLADDLGEQNDISAARPKKARKLRAKLDDYLQAVGARLPTR"
BASE COUNT          187 a          315 c          350 g          192 t
ORIGIN      
        1 ctgggtgaac gacgtgccct gctgcgcgat ggagtcgatg tgcggcgtga ggacatctcg
       61 cttgccgagg acgctcaggt ccgtgacgcc gagatcatcc gccacgatga tcacgacgtt
      121 gggcggcggc gcgccggccg aaacggaaac cggcgcgacg agggagaagg ccagcgccac
      181 ggcgccgaga acgaacgaat gtttcttcat ggcgtcgaaa ttagcgggtg ggcaaacgcg
      241 cgccgactgc ctgcagatag tcgtcgagct tggcgcggag cttccgcgcc tttttcggcc
      301 gcgcagcgct gatatcgttt tgttcgccca agtcgtcggc gaggtcgtag agctcgtcgc
      361 ggtcgttttc gtagaagtga atcaacgtgt agttgcccat gcgaatggcc gattgcggtc
      421 tcgtctgact ggtcttgaaa tcgtcgatcc cgatgtcggg cttggcctcg gcgaatttcc
      481 cctccgggtg ataatacggg aaatgccaga caatcggttg cgagcgctcg accgacttcg
      541 ccccgctcaa caagggcatg atgttgacgc cgtccatcgg cacgcccgcg ggcggtgtcg
      601 tgccggccac cgcgctgagc gtgggcatca agtcgctgcc gatgatcggc gtgtcggaga
      661 ccgaaccggc ggctacacgg cccggccaac gcacgatgaa cggcacgcgg atgccacctt
      721 cgtagacgtt ccacttgctg ccgcgcagcg gaccgttggc cgcatattcg gggtggccgc
      781 cgttgtcgga catgaagacg accagcgtat tctccgccac gccggcttca tcgatcgcct
      841 gcagcacgcg gccgacaaga tggtcgagtg tctcgaccat cccggcgtaa acggcgcgat
      901 cgggggaagc gtcggcgggg agtttcgcac gatacttctc cgtcagccac ggcgtgcgcg
      961 tgcggatcgg cgtgtgcacg taatactgcg acaaatacag gaagaagggg ccgtccttgc
     1021 gttcgtgaat gaacctcacg gcgc
//
LOCUS       DASPLT010000629         2119 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_47083, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000629 DASPLT010000000
VERSION     DASPLT010000629.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 2119)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2119)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2119
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_47083"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..241)
                     /locus_tag="VEA63_06510"
     CDS             complement(<1..241)
                     /locus_tag="VEA63_06510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD83683.1"
                     /translation="MARGRESGDAHRATLTAGDERIVKNDVRATNVCSLIDCSQRFAR
                     WILTLNPHHIMSSETPKSKTTTSANGRELTLTRLIH"
     gene            complement(196..582)
                     /locus_tag="VEA63_06515"
     CDS             complement(196..582)
                     /locus_tag="VEA63_06515"
                     /inference="COORDINATES: protein motif:HMM:NF024865.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HYD83684.1"
                     /translation="MSTNTVLADEVEKLNREWMQSYVRRDVAFLEKHLADDYTSTFPD
                     GAVLDKKGEIAALESGDVALAEMTPSEMNVRVYGSAAVITGRSTIKANVNGQEIGGEF
                     RFTDVWVKQDNRWLAVASQVTRIAQL"
     gene            674..964
                     /locus_tag="VEA63_06520"
     CDS             674..964
                     /locus_tag="VEA63_06520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD83685.1"
                     /translation="MADRADYLARTLIARLGNLEERFAEDERGEPDRRQRIETVEKVL
                     AVELGVADSATTALIEAAVPSSKTVKQGSDRELVEFAGFLRDRLRAHLSEQR"
     gene            complement(1051..1860)
                     /locus_tag="VEA63_06525"
     CDS             complement(1051..1860)
                     /locus_tag="VEA63_06525"
                     /inference="COORDINATES: protein motif:HMM:NF014016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CbiX/SirB N-terminal domain-containing protein"
                     /protein_id="HYD83686.1"
                     /translation="MSRRVLLMDNGSLEPASTLQLRRLAAALEAKIGVRVEPLSLAHS
                     AKVAASELDGRPAELFEAALDRVIGEGARDIVVAPLFVGPSHAVMRHVPSVVAERKKA
                     TPDLKVEIAAPLFVAGEQRLAEILADHAREEIKALATEARVAVVDHGSPSRAVTVVRD
                     AVAEQVRELLGSAVIEVAACSMERREGTEFAFNEPLLENLLAREGWRDGAVVVALLFI
                     GPGKHAGADGDVAQIVRRARAGKTDTVRFTPVMGLHPRLIEILADRVGPVS"
     gene            complement(1865..>2119)
                     /locus_tag="VEA63_06530"
     CDS             complement(1865..>2119)
                     /locus_tag="VEA63_06530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD83687.1"
                     /translation="ELVAGGLSEATPVAVVAGATLTSQSLRVGTLADGDGLATNLQGR
                     PAMIIVGEVVRWSELAAAAAGTVDEGRVAAADAVPMGCEI"
BASE COUNT          377 a          795 c          562 g          385 t
ORIGIN      
        1 catggatgag acgcgtcagc gtcagttcgc ggccgttggc cgaggtggtg gttttggatt
       61 tgggagtttc cgagctcatg atgtgatgtg ggttgagagt taaaatccag cgtgcgaaac
      121 gctgtgaaca atcaatcaac gaacaaacgt tcgtagcacg gacatcgttt ttaacgatcc
      181 gctcgtcgcc cgcggtcaga gttgcgcgat gcgcgtcacc tgactcgcga ccgcgagcca
      241 tcggttgtcc tgtttcaccc acacatccgt aaagcgaaac tctccgccga tctcctgtcc
      301 gttcacgttc gccttgatcg tgctccgccc ggtgatcacc gccgcgctgc cgtagacgcg
      361 cacgttcatc tcgcttggcg tcatctccgc gagcgccacg tcgcccgatt cgagcgccgc
      421 gatctcgcct tttttgtcga gcacggcgcc atcggggaac gtgctcgtat aatcgtcggc
      481 taaatgtttt tcgagaaaag ccacgtcgcg ccgcacatac gactgcatcc attctcgatt
      541 cagcttttcc acctcgtccg ccaaaacggt attcgtgctc atccgccttc tgaatgcgat
      601 tcgtccaccg aatcaagcga ggcgcttcga tacaggcggt tgaatttgtc gccggcctcg
      661 acgacatttt cgcatggctg atcgcgccga ctaccttgcc cgcaccttga tcgcacgtct
      721 cggcaacctc gaagagcgct tcgccgaaga cgagcgcggc gaaccggatc gtcgtcaacg
      781 catcgagacc gtcgagaaag tcctcgccgt ggaactcggc gtcgctgaca gcgccaccac
      841 cgcgctcatc gaagccgcgg tgccgagttc gaagactgtg aaacaaggat ctgatcgaga
      901 gctcgtcgaa ttcgccggat ttcttcgcga ccgcctccgc gcgcacctct ccgaacagcg
      961 ttaacgcagg aatgtagggc ggggtctccc gacctcgccg acactgcgct tccccccgcg
     1021 atcaggcggc gcccgcccgc tcgacgccgc tcaggacacc ggcccaaccc ggtccgccaa
     1081 aatctcgatc agccgcgggt gcagccccat cacgggcgtg aatcgaaccg tatccgtttt
     1141 cccagcacgc gcccgtcgca cgatttgcgc gacgtcgccg tcggcgcccg cgtgtttgcc
     1201 gggcccgata aacaacaacg ccaccaccac cgcaccgtcc cgccagccct cgcgcgcgag
     1261 gagattttcc aatagcggct cgttgaacgc aaactcggtg ccctctcttc gctccatcga
     1321 gcatgcggcc acttcgatca ccgcgctacc cagcagctct cgcacttgct ccgccacggc
     1381 atcgcgcacc acggtcaccg cgcgagacgg actgccgtga tcgaccacgg ccacacgtgc
     1441 ttcggtggcg agcgccttga tctcttcgcg cgcgtggtcg gccaggatct ccgccagccg
     1501 ctgttcaccc gccacaaaca acggcgccgc gatctccacc ttcaagtcgg gcgtcgcttt
     1561 cttgcgctcc gcaaccaccg acggcacatg ccgcatcacc gcgtgcgacg gtccgacaaa
     1621 cagcggcgct accacgatat cgcgtgcgcc ctcaccgatc acgcgatcga gcgccgcctc
     1681 gaacaactcc gccggtcgtc cgtccaactc cgacgccgcc actttcgccg aatgcgccaa
     1741 cgacagcggc tccacgcgca cgccgatttt cgcctccagc gccgccgcca gtcgcctcag
     1801 ttgcaacgtg ctcgccggct ccagcgagcc gttgtccatc agcagcacgc gccgcgacat
     1861 cgcgttagat ttcgcagccc atcggcaccg catccgccgc cgcgacccgg ccttcgtcaa
     1921 cggttcccgc tgccgccgcc gcgagttcgc tccaacgcac cacctcgcca acgatgatca
     1981 tcgccggccg tccttgcaga ttcgtcgcca acccatcgcc atccgccagc gtgccgacgc
     2041 gcaaactctg cgacgtcaac gtcgctcccg cgaccaccgc caccggcgtc gcctccgaca
     2101 acccgcccgc aaccagctc
//
LOCUS       DASPLT010000630         3539 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_273421, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000630 DASPLT010000000
VERSION     DASPLT010000630.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 3539)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3539)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3539
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_273421"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1202)
                     /locus_tag="VEA63_06535"
     CDS             complement(<1..1202)
                     /locus_tag="VEA63_06535"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HYD83688.1"
                     /translation="MTTLVPPHICLRKKRLFHGVLAGMLSLLLGSISLHAQEGRGSIQ
                     GRVQDPASGDYIANAKVVVAETGQEALTDSTGFYQFTGVRAGDATLRVSYLNFDEMTV
                     QVPVTEGELVVHDFALTNKTRYGTDDSVVSLDPFIVATTREEEASGIAIAEQRNAHIQ
                     KKVLAADAFGEISENNVGEFLKRMPGVTVNYSDGDAASISLRGFSDMHTSILIDGNSV
                     ASGGSSNPTRLIDLENLSVGNASRLEVIKTVLPNQWANSLGGSVNLVSKSSFERTRPL
                     LIARAVVQFTNDDHSFGRSPGPGWKNTNKVRPGGGFSYVNPISINLGITMSANYSDQF
                     GRRTTSQTGYEFVAGTNNATGGSETEPYLRTLRLSNNERSTTREAYSFGVDWRPIERL
                     TLNFNYQY"
     gene            complement(1485..>3539)
                     /locus_tag="VEA63_06540"
     CDS             complement(1485..>3539)
                     /locus_tag="VEA63_06540"
                     /inference="COORDINATES: protein motif:HMM:NF039707.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6259 domain-containing protein"
                     /protein_id="HYD83689.1"
                     /translation="ADGTRILEPADATRCEVESLGGPTPGLRLSWSEFSGDDVAGVRV
                     EIVARLTDDPAVSRWEIVVTKPRDCVVQQVVFPRLAGIPAQSDERLAVPAWLGELLVD
                     PRNAMVREAGKSARTEWQYPGRLAMQCLAFYRPNAAGLYLACDDSTALRKIFTANADG
                     HGGFGLEIAHLPENGARGTERYTLPYGVQMGTFQGDWITAAERYRTWAMQQSWVKASR
                     LASGAVPEWAQETALWVWNRGRSKDVLAPAVAMQRELGLPVSVLWHWWHGGAYDADFP
                     EYLPPREGTESFRAAMADAHRHGVHSLVYMNTRLWGLTTRSWEEEGAVAFAVKRPDGS
                     HRREVYNTFTRQPLTAMCMGTEFWRNKYAGLAERAVRELGVDGIYMDQACSSLSCFDP
                     THGHPLGGGSYWINGFRQLAADIRHRSAGVRAIGLAGEGAGEAWLPHLDLMLSLEVSR
                     ERYRPPTPAWEPIPFFQAVYHPYAIQFGNYSSLTMPPYDDLWPAEFAPKQPLQLLDEK
                     FTPQFRYEQARAFVWGQQPMIANFLPSHVQDRAQEIAYVLRLARLRHAGLDYLMHGEF
                     LRPPAIDAPSIEADISRLSIYAGQKGALTSFKKHLPQAIAGAWRSPSGNVAVALASIA
                     DEPLTLTLAFDPAYHGVAGAKHIVRIDESGRTPLGRLSETGTLAVTLPARGACLVEFE
                     RQ"
BASE COUNT          677 a         1155 c         1032 g          675 t
ORIGIN      
        1 ttgtattgat agttaaagtt aagtgtcagc cgttcaatgg gccgccaatc gacgccaaaa
       61 ctataggctt cccgcgtggt gctgcgttcg ttgttgctca gacgcagagt gcgcagatac
      121 ggctccgttt cagatcctcc ggtggcattg ttggtgccag ctacaaactc gtaacccgtc
      181 tgcgaggtcg tgcgccgccc aaactgatcc gagtaattgg cgctcattgt gatgcctaga
      241 tttatgctga tcggattgac ataggagaat ccgccgccgg gacgcacctt gttcgtgttt
      301 ttccatcccg gccccgggct gcgaccaaag gaatgatcat cgttggtaaa ctgcaccacc
      361 gcgcgcgcga taagcagcgg ccgcgtccgc tcaaatgaac tcttgctcac aaggttcacg
      421 gaacctccca gtgaattcgc ccactggttt ggcaataccg tcttgatgac ctccagcctc
      481 gaggcgttgc ccacggaaag attctccagg tcaatcaagc gggtcggatt ggaggagcct
      541 ccgctcgcga cgctgttacc gtcgatcagg atcgaggtgt gcatgtcgct aaagccccgc
      601 agactgatgc tggccgcgtc cccatcggaa tagttgacgg tgacgccggg cattcgtttg
      661 aggaactcgc ctacattatt ctcgctgatc tcaccgaatg catcggccgc caaaaccttc
      721 ttctggatat gtgcattccg ttgttcggcg atcgcgatgc ccgacgcctc ttcttcgcgc
      781 gtcgtcgcga cgatgaaagg atcgaggctg actacagaat catccgtccc atatcgcgtt
      841 ttgttggtga gcgcgaagtc atgcacaacc agttcccctt ccgtcacggg aacctgcacg
      901 gtcatctcgt cgaaattgag gtagcttacc cgcagagtcg catcgccggc gcgaactccg
      961 gtaaactggt aaaaccccgt ggaatcggtg agggcttcct gcccggtctc cgccacgacg
     1021 accttcgcgt ttgcaatata gtctccggaa gcggggtcct ggactcgacc ttgaatggaa
     1081 ccacggccct cctgagcgtg cagcgagatg ctgcccagca ggaggctcaa catgcctgcc
     1141 aaaacgccgt gaaacaggcg tttcttgcgc aggcaaatat ggggtggaac aagagtagtc
     1201 atcagcaagg ggtgttgggg ttgatcaatg aagcgagtga cgcattgagg ctgctccgtc
     1261 gcgtcaaacg cgggaaggcg ggcatcgtgg taactgccgt ttaccacttc gcgaacgtga
     1321 catgcgcgat gtgcttcaac ccacggttta cttcagcgaa cggctacgtc gacggaaggc
     1381 gccgatccgg ccgtcctcat gttcaccgtg tcggcgggga tgtgtccggg gagccagcgc
     1441 atcagcccca gccgcaatca cggcgcggct atgcgcgccc agtcttactg ccgctcgaac
     1501 tccaccaagc acgcgccacg cgccggcagc gtcacggcaa gcgtgcccgt ctcgctcaac
     1561 cgtccgagcg gcgtgcgacc cgactcgtcg atgcgcacga tgtgtttcgc gccggccacg
     1621 ccgtgatacg ccggatcgaa cgccagcgtg agcgtcagcg gttcatccgc gatgctggcg
     1681 agcgccacgg cgacgttgcc cgacggcgaa cgccatgctc cggcgatcgc ttgcggcaaa
     1741 tgtttcttga acgacgtcag cgcgcccttc tggcccgcgt aaatcgagag acgcgagatg
     1801 tcagcttcga tcgagggagc atcaatcgcc ggcggtcgga gaaattctcc gtgcatgaga
     1861 taatcgaggc ccgcgtgccg cagccgcgcg agccgtagca cgtaggcgat ttcttgcgcg
     1921 cgatcctgca cgtgcgacgg caggaaattc gcaatcatcg gctgctgtcc ccacacgaaa
     1981 gcgcgcgcct gctcgtagcg aaactgcggc gtgaactttt cgtcgagcaa ttgcagcggt
     2041 tgctttggcg cgaactccgc cggccacaaa tcgtcgtaag gcggcatcgt gagtgacgag
     2101 tagttcccga actgaatcgc atatggatga tacaccgcct gaaaaaacgg gatcggctcc
     2161 cacgccggcg tcggcggacg atagcgttcg cggctcacct cgaggctgag catgagatcg
     2221 agatgcggca gccacgcctc gcccgcgcct tcgccggcca acccgatcgc gcgcacgccc
     2281 gcgctccggt gacgaatgtc cgccgcgagc tgacgaaatc cgtttatcca ataactgccg
     2341 ccaccgagcg gatggccatg cgtcggatcg aaacaggaaa gactgctgca cgcctgatcc
     2401 atgtagatgc cgtcgacgcc gagctcgcga accgcgcgtt cggccaggcc cgcgtattta
     2461 ttgcgccaga attccgtgcc catgcacatg gccgtgagcg gctgacgcgt gaacgtgtta
     2521 tacacttcgc gacggtgact gccgtcgggc cgtttcaccg caaacgccac cgcgccttcc
     2581 tcctcccagc ttcgcgtcgt gagaccccac aaacgcgtgt tcatgtaaac cagcgaatga
     2641 acaccgtgac gatgcgcgtc ggccatcgcg gcgcgaaacg attccgttcc ttcgcgcggc
     2701 ggaagatact ccgggaaatc cgcgtcgtag gcgccgccgt gccaccaatg ccacaacacg
     2761 ctcaccggca gcccgagttc gcgctgcatc gcgaccgccg gcgcgagcac gtctttcgag
     2821 cgtccgcggt tccaaaccca tagcgcggtt tcctgcgccc attccggcac ggcgccactc
     2881 gcgagccggc tcgctttcac ccacgactgc tgcatcgccc acgtgcgata acgttccgcc
     2941 gcggtgatcc agtcgccctg aaacgtgccc atctgcacgc catacggcag cgtataacgc
     3001 tccgtgccgc gcgctccgtt ctccggcaga tgcgcgatct ccaaaccgaa gccgccgtga
     3061 ccatcggcat tcgcggtgaa aatttttctt agcgcggtcg aatcgtcgca cgccagatag
     3121 agccctgcgg cgttcggtcg ataaaacgcc agacactgca tcgcgaggcg ccccggatac
     3181 tgccattcgg tgcgcgcgct tttgccggct tcgcggacca tcgcgttgcg aggatccacc
     3241 aataattcgc cgagccacgc cggcaccgcg agccgctcgt cggattgcgc gggtatccct
     3301 gcgagccgcg gaaacaccac ctgctgcacc acgcaatcgc gcggttttgt caccacgatc
     3361 tcccagcggc tcaccgccgg atcgtcggtc aaacgtgcca cgatttcgac gcgcacaccc
     3421 gcgacgtcgt ctccggaaaa ctcgctccaa ctcaaccgca ggccgggcgt cgggccgccg
     3481 agcgattcga cttcgcagcg cgttgcgtct gctggctcga ggattcgcgt gccgtcggc
//
LOCUS       DASPLT010000631         1092 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_31379, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000631 DASPLT010000000
VERSION     DASPLT010000631.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1092)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1092)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1092
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_31379"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            51..>1092
                     /locus_tag="VEA63_06545"
     CDS             51..>1092
                     /locus_tag="VEA63_06545"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HYD83690.1"
                     /translation="MTRLLYEISEAFKIAFAQLRANKMRSVLTALGVIIGIVAVTLMG
                     TAIRGIDIGFQRSLAMLGDDVLYVQKWPWHGVEDWWNYENRPLIRAEQAVTLNRIIDA
                     TPNSLLEVAVPVVARPAGVKAGDNQVSGVYIVGTTADYSRLLSVDFADGRFFSETEAA
                     GGRQVCLLGYDVAEALFPSGHAIDQKVLIRGQPFTVIGVFARQGRFLGLFSFDTHAII
                     PIAAYRKHFGVGWRGADLRVKVRDKNQLTSEVEELTGAMRRVRGQLPGERDNFSINQQ
                     QAFKAQLDPVKSGIALAGFVITGLALFVGAIGIMNITFVSVKERTKEIGTRKALGARR
                     RTILLQFLVESAV"
BASE COUNT          199 a          306 c          372 g          215 t
ORIGIN      
        1 agttgtatct gaatcgctga atctccggcg aagcgagaaa ccgcccgaac atgacccggc
       61 tgctctacga gatttccgag gcattcaaga tcgcattcgc gcaactgcgc gcgaacaaga
      121 tgcgctccgt cctcaccgcc ctcggcgtga tcatcggcat cgtggcggtc acgctgatgg
      181 gcacggcgat ccgtggcatc gacatcggtt tccagcgcag cctcgcgatg ctcggcgacg
      241 acgtgcttta cgtgcagaaa tggccgtggc acggcgtcga ggactggtgg aattatgaga
      301 accgtccact catccgagcg gagcaggcgg tgacgttgaa ccggatcatc gacgccacgc
      361 cgaactccct gctcgaggtc gcggtgccgg tggttgcgcg gccggcggga gtgaaggcgg
      421 gcgacaatca agtgagcggc gtctatatcg tcggcacgac cgcggactat tcacgactgc
      481 tgtcggtcga tttcgccgat gggcgttttt tcagtgaaac ggaagccgcg ggcgggcgcc
      541 aggtgtgttt attgggctac gatgtcgcgg aggctttgtt tccgagcggg catgcgatcg
      601 accagaaagt gctgatccgt gggcagccat ttaccgtgat cggcgtgttc gcccgccagg
      661 ggcggtttct cggactgttt agctttgata cgcacgcgat catcccgatc gcggcgtatc
      721 ggaaacattt tggcgtcggc tggcgcggcg cggatctgcg cgtgaaggtg cgagacaaga
      781 atcaactgac gtcggaggtg gaggagctca cgggcgcgat gcggcgcgtg cgcgggcagt
      841 tgcccggcga gcgcgataat ttttcgatca accagcaaca ggcgttcaag gcgcagctcg
      901 atccggtgaa gtcgggcatt gcgctggcgg gtttcgtgat cactgggctc gcgctgtttg
      961 tcggtgcgat cggcatcatg aacatcacgt ttgtcagcgt aaaggaacgc acgaaggaga
     1021 tcggcacccg aaaggcgctc ggcgcgcggc ggcgaacgat cctcctccag ttcctcgtcg
     1081 agtccgcggt gg
//
LOCUS       DASPLT010000632         2998 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_192677, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000632 DASPLT010000000
VERSION     DASPLT010000632.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 2998)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2998)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2998
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_192677"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            248..2197
                     /locus_tag="VEA63_06550"
     CDS             248..2197
                     /locus_tag="VEA63_06550"
                     /EC_number="4.2.1.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjhG/YagF family D-xylonate dehydratase"
                     /protein_id="HYD83691.1"
                     /translation="MSDPILDSANEAIYTLRTTAPGPGGQLPVTPDRLRAMSSGELFG
                     WTQNAGMGWDPKLLLGKQFLILSTQGGIRAPDGSPIALGYHTGHWEVGLLMEEAARTF
                     SAAKAIPFAGYVSDPCDGRTNGTAGMLDSLPYRNDAAVVLRRLIRSLPTRRGVLGVAT
                     CDKGLPAMLMALAGSPDLPTILVPGGVTLLAEQAEDTAKVQTLATRFARDEISLDYAA
                     DMGCRACGSPGGGCQFMGTAATSQVVAEALGLSLPHAALSPSGAEIWRDLARRSATAL
                     MALDSAGLTTRDILSDDSIHNAMVAHAAIGGSTNLVLHIPAFAHAAGLQRPTVHDWTR
                     INRSVPRLVDSLPNGPRHFATVQVFMAGGVPEMMLHLRELGLLKLDARTVTGRTLGEN
                     LEWWEKSERRQRLRAKLTQLDGIDPLDVIMPAPRARERGLKSTVTFLRGNLAPEGALV
                     KSTSILPERLDASGVYHHEGPARVFTSENSAIAAIKSGSVKAGDIMVLIGIGPGVGMP
                     ETYQITSALKYVKDGAKIALITDGRFSGVSTGACIGHASPEAWAGGPIGQVRNGDILR
                     LHIDTNSLEGSVDVISVEQSEFLARSRHPELRRDERVPADTRLWAALQDASGGSWAGC
                     VYDVDRIAHLLELGKAAEAKSKAGL"
     gene            complement(2289..>2998)
                     /locus_tag="VEA63_06555"
     CDS             complement(2289..>2998)
                     /locus_tag="VEA63_06555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="family 16 glycoside hydrolase"
                     /protein_id="HYD83692.1"
                     /translation="NQLYFDAIKDQPKVTLLDTWSLFADAKGDAKEDEFPDLLHPNGV
                     GYAKWEKALRPIMETVGLLAAWPDDFAPEPGFVSLLNGRDLSGWGYKGQPALDGKTVS
                     DDGRYLAKNGRLVVTVAHTERDYKTLWTTRTFPKDFELRLEFRASPNADSGVFIRQPQ
                     LQCRDYLIAGPYKDLKKYRPLEWNELVVTVKGALAHCTCNGEVLIDAMPVPADGPIGL
                     ESDRGQMEYRRIRVKEL"
BASE COUNT          532 a         1079 c          857 g          530 t
ORIGIN      
        1 ccgtctgcta ccagaacttc cccgacgcgc tgctgccggc cgaacttcaa aacgccaatc
       61 cgctcggcct gctgcgactc gtcgacggca aatactcccg cgatccgatt cgttgaggcg
      121 aactgaagct ccaagcacca acatccaagc tccagagaat acccaagctc cagttcagat
      181 ccagttttga ggtttgaagt ttgaaacttc cttggagctt ggtccttgga gcttggagct
      241 tttccccatg tccgatccca tcctcgattc cgccaacgaa gccatctaca ccctgcgcac
      301 caccgcgccc ggtcccggcg gacaactccc cgtcacaccc gatcggctcc gcgcgatgag
      361 cagcggagag cttttcggtt ggacgcaaaa cgccggaatg ggttgggatc cgaagctgct
      421 cctcggaaaa cagttcctca tcctgagcac gcaaggcggc atccgcgcgc ccgacggttc
      481 gccgatcgcg ctcggttatc acaccggcca ttgggaagtc ggcctcctga tggaggaagc
      541 cgcgcgcacg tttagcgccg cgaaggcgat tccgttcgcc ggctatgtca gcgacccatg
      601 cgacggccgc accaacggca ccgccggcat gctcgacagc ctgccttacc gcaacgacgc
      661 cgccgtcgta ttgcggcggc tgatacgctc gctgcccacg cgccgcggcg tcctcggggt
      721 cgcgacgtgc gacaaaggtc ttcccgcgat gctcatggcg ctggccggct cgcccgatct
      781 gccgacgatt ctcgtcccgg gtggcgtcac gttgctcgcc gaacaagcgg aggacaccgc
      841 caaggtgcag acgctcgcca cgcgtttcgc gcgcgacgaa atctcgctcg attacgccgc
      901 cgacatgggc tgccgcgcgt gcggctcgcc gggcggcggc tgtcaattca tgggcaccgc
      961 cgcgacctcg caggtcgtcg ccgaggccct cggcctcagc ctgccgcacg ccgcgctcag
     1021 tccgtccggt gcggagattt ggcgcgacct cgcccgccgc tcggcgaccg cattgatggc
     1081 actcgattcc gccggactca cgacgcgcga catcctgagc gacgattcga ttcacaacgc
     1141 catggtcgcg cacgccgcga tcggcggttc gactaatctc gtgctgcaca tcccggcctt
     1201 cgcgcacgcc gcgggtttgc aacgtcccac cgtgcacgat tggacgcgca tcaatcgctc
     1261 cgtcccgcgc ctcgtcgatt cgctgcccaa cggcccgcgc cacttcgcca ccgtgcaggt
     1321 tttcatggcc ggcggcgtcc ccgagatgat gcttcacttg cgcgagctcg ggttgttgaa
     1381 actcgacgcg cgcaccgtca ccggtcgcac gctcggcgaa aatcttgagt ggtgggaaaa
     1441 gtccgaacgc cgccagcgcc ttcgcgcgaa gctcacgcag ctcgacggaa tcgatccgct
     1501 cgacgtcatc atgcctgcgc cgcgcgcgcg cgagcgcggc ctgaagagca cggtgacatt
     1561 tctccgcggc aatctcgccc cggaaggcgc gctcgtgaag agcacctcga ttctccccga
     1621 gcgcctcgac gcgagtggcg tttaccatca cgaaggcccg gcgcgcgtgt tcaccagcga
     1681 gaactccgcg atcgccgcga tcaaaagcgg cagcgtcaaa gccggcgaca tcatggtgct
     1741 gatcggcatc ggccccggcg tcggcatgcc cgaaacgtat caaatcacgt ccgcgctgaa
     1801 atacgtgaaa gacggcgcga aaatcgcact gatcaccgac ggccgtttct ccggcgtctc
     1861 caccggcgcg tgcatcggcc acgcgagccc cgaagcgtgg gctggcggtc cgatcggcca
     1921 ggtgcgcaac ggcgacattc ttcggcttca catcgacacc aacagcctcg aaggctcggt
     1981 cgacgtgatc agtgtcgagc aaagcgaatt cctcgcgcgt tcgcgccacc ccgaattgcg
     2041 tcgtgacgaa cgcgtccccg ccgacacgcg cctctgggct gcgctgcaag acgcgagcgg
     2101 cggcagctgg gccggctgcg tttacgacgt cgaccgcatc gcgcacttgc tcgaactcgg
     2161 caaagccgcc gaagccaaaa gcaaagcggg tctctgaccc acccttccgt gtagccctgc
     2221 gcgtgagcgc agggaccggg tgctgcgaac ctcccgctgc tcacgcagcg agccacgcca
     2281 gatcgcgctc acaactcctt cacgcggatc cggcgatact ccatctgccc gcgatcgctc
     2341 tcgagtccga tcggtccgtc agccggcacc ggcatcgcgt cgatcagcac ttcgccgttg
     2401 cacgtgcagt gcgcgagcgc gcccttcacc gtcaccacga gttcgttcca ttcgagcgga
     2461 cgatactttt tcaaatcctt gtaaggcccc gcgatcaagt agtcgcggca ttgcagttgt
     2521 ggttgccgaa tgaatacacc gctgtccgcg ttcggactcg cgcgaaactc cagccgcagc
     2581 tcgaagtcct tcggaaaggt ccgcgtcgtc cacagcgttt tgtaatcacg ctccgtgtgc
     2641 gccaccgtca ccacgagccg cccgttcttc gccagatagc gcccgtcatc gctcaccgtt
     2701 tttccatcga gcgccggctg acctttgtaa ccccagccgc tgagatcgcg gccgttgagc
     2761 aagctcacaa acccgggctc cggcgcgaaa tcgtcgggcc acgccgccaa caaccccact
     2821 gtctccatga tcggtcgcag cgccttttcc catttcgcat aaccgacgcc gttcggatgc
     2881 agcaaatcgg ggaactcatc ctccttcgca tcgcctttcg cgtccgcgaa caacgaccac
     2941 gtgtcgagca gcgtcacctt cggctgatct ttgatcgcgt cgaaatacaa ctggttca
//
LOCUS       DASPLT010000633         4258 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_47141, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000633 DASPLT010000000
VERSION     DASPLT010000633.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 4258)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4258)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4258
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_47141"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1183)
                     /locus_tag="VEA63_06560"
     CDS             complement(<1..1183)
                     /locus_tag="VEA63_06560"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HYD83693.1"
                     /translation="MNYTRNLLPEICFRLLTAVLLAASAVLDAQPTDAAAAEPVMLDV
                     FTVSSATDQGYKASNAVGATRTNTPIADTPQSIGVFNDLFIEDTMSFNLTELTLYDPT
                     FMPTQADKGTYLARGFTAASTATFVDGLPQRNTFGPNYTANVDRVEILKGPAAILYGQ
                     GPAGATINRVLKRAQAKQRTTLTTTASDDGTLRAHLDTTGPLALRIGGRPLAYRLNLA
                     VQEGETFKLQSEDNEILFSPALHIPLGRHTDLDLNFTYDKTNLTGNFAHPVTGGVPGR
                     VILTDSTALQVPIENSFGEPYDERPIEKTLTSYDLRHQFSGHLSFRSQYQFETYNAEI
                     SELFPQIGQYVITATSASIRRVYREQNTDMNADRTRNEFVADFATGPVHHKALAGFSF
                     DE"
     gene            complement(1339..2439)
                     /locus_tag="VEA63_06565"
     CDS             complement(1339..2439)
                     /locus_tag="VEA63_06565"
                     /inference="COORDINATES: protein motif:HMM:NF024770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HYD83694.1"
                     /translation="MHPIPQRHSLVSQTAQVLRDEIAKGTWSEWLPGERMLCEMLQVS
                     RNTLRAALTQLQREGSIAPKHGAGNRVVRSGKRAAKRAPAAGARDVGLLSPEPLERLR
                     PTQTLWIDELRALLSERDCRLHVFHGPQYFRREPGPALQRLVAQRVHGCWILTLSNEA
                     IQRWFSENGVSCIVAGSVHAGLGLPTRDLDHRALCRHAAGVLLGAGHRQLALVIGQSR
                     LAGDLASETGFLEGVQTSRAAAEAAAVVARHDGTVAGISNALRRLMSAKRPPTGLLVA
                     NAYHYLTVASRLAEQGIRVPQDVSLISRDEDPFLSFMVPEPARYIISPRTLSKALLEP
                     VIDLLEHRPVARPVVQLMPEFLRGASIAPSRV"
     gene            2859..>4258
                     /locus_tag="VEA63_06570"
     CDS             2859..>4258
                     /locus_tag="VEA63_06570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HYD83695.1"
                     /translation="MKATTPHRISLAILLACTVPFLSAQTADNRDAATNEEEKEEEVV
                     KLSPFEVVSTEDNGYAATSSLAGSRLNTQLRDVASAITVVTPEFLKDTGSTNLRDVLV
                     YTTSTEVSGIGGNFYGGNADDNAYRNQMLANPQSGTRVRGLNTADLTRNFFVTSIPMD
                     AYNTSRIDIQRGPNSILFGLGSPAGIINNTLKDPNLEKLEAEAQFRVGSYGSHREVVD
                     VNVPLIKGQLGLRVAGLNDEAQYRQDFTFNHDKRLYGALRWQPKLAQHVFTQFDIKAE
                     KGEIRGNRPIATTAADFITNWFGPLNKYLMYDPLTSNGIPQEPNGTQHRELSHYFAGA
                     PARDWWNSSPATIYQNPSAAAIGNGQLDAYRQRDGSPWGGLSGVTNPNLDEGGTGSWN
                     RNTAAFFAGNSIISDLISDYESTSGRAFNGFGAALWPTQMILSGPLAFVDRTMAGPNK
                     GEWNEFDTIDLSFTQT"
BASE COUNT          858 a         1365 c         1280 g          755 t
ORIGIN      
        1 tctcgtcgaa cgagaagccg gcgagcgcct tgtggtggac cggaccggtg gcaaagtcgg
       61 cgacgaactc gttgcgcgtg cgatcggcgt tcatgtcggt gttctgctcg cgatacacgc
      121 ggcgaattga cgcgctggtg gcggtgatca cgtattggcc gatctgtgga aacagctcgc
      181 tgatttcggc gttgtaggtc tcgaactgat actgcgaccg gaaggaaaga tgcccgctga
      241 actgatggcg aagatcgtag ctcgtcagcg tcttctcgat cggacgttcg tcgtagggtt
      301 ctccgaagga attttcgatc ggcacctgca acgccgtgct gtcggtcagg atcacgcgac
      361 cgggcactcc gccggtcacc ggatgtgcga agttcccggt caggttcgtc ttgtcgtagg
      421 tgaagttcag atcgagatct gtgtgacggc cgagcggaat atgcagcgcg ggcgaaaaga
      481 gaatttcgtt gtcttcgctt tgcagcttga aggtctcgcc ttcttgaact gcgagattca
      541 agcggtaggc aagcgggcgg ccaccgatgc ggagcgcgag cggcccggtt gtgtcgaggt
      601 gtgcgcgaag cgtgccatca tcgctcgccg tcgtcgtcaa cgtggtcctc tgtttcgcct
      661 gcgcgcgttt cagcacgcga ttgatcgtgg ccccggcggg gccttggccg taaaggatcg
      721 ccgcggggcc tttcaggatt tccacgcgat cgacgttggc ggtgtagttg ggaccaaacg
      781 tgtttcgctg cggaaggccg tccacgaacg tcgcggtcga cgccgccgta aacccgcgcg
      841 cgaggtaagt gcctttatcc gcctgcgtcg gcatgaacgt cggatcgtag agtgtgagct
      901 cggtcagatt gaacgacatc gtgtcctcga taaagagatc gttgaaaacg ccgatcgact
      961 gcggtgtatc cgcgatgggc gtattggtgc gcgtcgcgcc aacggcgttg gaggccttgt
     1021 agccttggtc ggttgcggag ctgacggtaa acacgtccag catcaccggc tccgcggccg
     1081 ctgcatccgt gggttgagca tcgagcacgg ccgatgccgc gagcagcacc gccgtcagga
     1141 gccgaaagca aatttcagga aggaggtttc gagtgtagtt catcgcgaga acgataagga
     1201 tcaggggttc gaaccgcggg gatggggacc cgttaagctg cggttgaaag cgtaggtaag
     1261 gagcgccacg cgtcggcgac atccccgatc agtggtccca tcgagggacc acttcgctcg
     1321 tcctacctct ctcgtcatct agacccggct cggcgcgatc gacgcgcccc gcaaaaactc
     1381 cggcatgagc tgcaccacgg gccgcgccac cgggcggtgc tccagcaaat cgatgacggg
     1441 ctcgagcaac gctttggaga gcgttcgcgg actgatgatg taacgcgcag gctccggcac
     1501 catgaacgag agaaagggat cttcgtcgcg cgagatcagc gagacatcct gaggcacccg
     1561 gatcccctgt tccgcgagcc ggctcgcgac ggtgagatag tgataagcgt tggcaacgag
     1621 caagccggtc ggaggtcgtt tggcgctcat caaccggcgc aacgcattcg aaattcccgc
     1681 aacggtgccg tcatgtcgcg cgaccaccgc ggcagcctcg gctgcggcac gcgaagtctg
     1741 cacgccttcg agaaatccgg tttcgctggc gagatcgccg gcgagccggg attgtccgat
     1801 cacgagcgca agttgacgat gaccggcgcc cagcaacaca cccgcggcat gccggcacaa
     1861 cgcgcgatga tcgagatcgc gcgtcggcaa accgaggccc gcatgcacag agcccgcgac
     1921 gatgcagctt acgccgttct cgctgaacca gcgctgaatc gcctcgttcg aaagcgtcag
     1981 gatccaacag ccgtgcacgc gctgagcgac cagtcgctgg agcgcgggtc ccggctcgcg
     2041 gcgaaaatac tgggggccgt ggaaaacatg cagccggcaa tcgcgctcgc tcagcaacgc
     2101 gcgcagttcg tcgatccaca aagtttgagt cggacgcagc cgctcgagcg gttcgggaga
     2161 aagcaacccg acgtcgcgcg cgcccgccgc cggcgcccgt ttcgcggcgc gtttcccgga
     2221 tcgaacgacc cgattacctg caccgtgttt cggcgcgatt gagccttccc gctgcaactg
     2281 cgtgagcgcg gcgcgcagcg tgttgcggct gacctgcagc atctcgcaga gcatgcgttc
     2341 gcctggcagc cactcgctcc acgtcccttt cgcgatctcg tcccgcaaga cttgcgcagt
     2401 ctggctgacc agagaatggc gctgcggaat cggatgcatc cgcggcacct acgccgcctc
     2461 gagcgccgca ggcgagcacg aactttccgt cacacgtcgc gtcgagacaa gcgaagggac
     2521 tcacaggctc tggcgaaaaa atttcgccaa tcattccgaa gggaagggaa catcatcggg
     2581 gaatacccga cattaacccg atgcagtcgc acgcgctgca caatttcgcc cgatgcacgc
     2641 gcgattgcct gcaatgaatg gaccagcgac gtccacccca acgtcttttc cgatcgcaga
     2701 aaagattcac ccaaacaggt gatagccgga gatgtctgag atgcgttagc atgcggccgt
     2761 tatcccgtcg ttccccctcc ccgcgcgacc tgcggactcg ctccggctac ggcgattgtt
     2821 cgcgcatttt tcccaccccg tacaacaggc aaacacacat gaaagcgact accccacatc
     2881 ggatctcact cgcgattctg ctcgcgtgca ccgttccgtt tttgtccgcg caaaccgcgg
     2941 ataatcgcga cgccgcaacc aacgaagaag aaaaagaaga agaggtcgtg aagctctcgc
     3001 cgttcgaagt cgtcagcacc gaagacaacg gctacgcggc cacctcctcg ctcgccggct
     3061 cgcgtctcaa cacgcagctc cgcgacgtcg cttcggcgat cacggtcgtt acgccggaat
     3121 tcttgaagga caccggttcc accaatctgc gcgacgtcct tgtctacacg acgagcaccg
     3181 aagtctcggg catcggcgga aatttctacg gcggcaatgc cgacgataac gcctaccgca
     3241 accagatgct cgcgaacccg cagagcggca cgcgcgtgcg cggcctcaac accgccgacc
     3301 tcacgcgcaa ttttttcgtc acgagcatcc ccatggatgc ctacaacaca tcgcgcatcg
     3361 acatccagcg cggccccaac tccatcctct tcggtctcgg cagcccggcc ggcatcatca
     3421 acaacaccct caaggatccg aatctcgaga agctcgaagc ggaggcgcag ttccgcgtcg
     3481 gcagctacgg ttcccatcgc gaagtcgtcg atgtgaacgt gccgctgatc aagggacaac
     3541 tcggcttgcg cgtggcaggc ttgaacgacg aagcgcaata ccgacaggac ttcacgttca
     3601 accacgacaa gcggctctac ggcgcgctcc gttggcaacc caagctcgct caacacgtgt
     3661 tcacgcagtt cgacatcaaa gccgagaagg gcgagatccg gggcaatcgt ccgatcgcca
     3721 ccaccgccgc cgacttcatc acgaactggt ttgggccgct caacaaatac ctgatgtatg
     3781 atccgctcac gagtaacggc atcccgcagg agccgaatgg cacgcagcac cgcgagctgt
     3841 cgcactattt cgccggcgcg cccgcgcgcg attggtggaa cagctcgccc gccaccatct
     3901 atcaaaaccc atcagccgcc gcgatcggca acggccagct cgacgcctac cgccagcgcg
     3961 acggttcgcc gtggggtggc ctcagcggcg tcaccaatcc aaacctcgat gaaggtggca
     4021 ccggatcgtg gaaccgaaac accgccgcct tcttcgccgg caactcgatc atctccgacc
     4081 tcatcagcga ttacgagagc acgtcggggc gcgcgttcaa cggtttcggc gccgcgcttt
     4141 ggcccacgca gatgatcctc agcggtccgc tcgctttcgt cgaccgcacc atggctggcc
     4201 cgaacaaggg agaatggaat gagttcgaca ccatcgacct ctccttcacg cagaccta
//
LOCUS       DASPLT010000634         1407 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_232619, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000634 DASPLT010000000
VERSION     DASPLT010000634.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1407)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1407)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1407
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_232619"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..814)
                     /locus_tag="VEA63_06575"
     CDS             complement(<1..814)
                     /locus_tag="VEA63_06575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HYD83696.1"
                     /translation="MFGLFASQEKKARENAGNWLELADKVWHFRRDLLSDKESAELLK
                     RAAELREQLKAKADAARLKLSIESLEAVLRRTGGAIYPKTSLVENVEFFLVAAIVILG
                     IRTYFVQPFKIPTNSMWPTYFGMTPEVFTSPADEPNKLEQAGRLLAFGAWPHRLDAPA
                     SGEVLIPVGGIESRGIVHSRIVRGRSWLVLPTTLREYTLLVGSEAVRVRVPMDFDFDW
                     AVYDGFFSDGGHYSSRKLASEIQRRLETGQVEVRVIDGERVRCVRTGRRVREG"
     gene            complement(828..>1407)
                     /locus_tag="VEA63_06580"
     CDS             complement(828..>1407)
                     /locus_tag="VEA63_06580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376004.1"
                     /note="back-translocating Elongation Factor EF4; binds to
                     the ribosome on the universally-conserved alpha-sarcin
                     loop; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HYD83697.1"
                     /translation="KAAIHIPNDAMGDILALVMEKRGVVDHTDTLDANRVMLTCLLPL
                     NEILVDFNDRLKSITHGYGSMDYELGEYRTSDLVKMEILINGDPVDAFASIVHREKAE
                     GKGRELCEKLAEIIPPQMFKVAIQAAIGGKVIARDNVKEMRKDVTSKCYGGDISRKRK
                     LLDKQKEGKKKMKQFGRVSIPSDAFIKVLKTN"
BASE COUNT          260 a          458 c          390 g          299 t
ORIGIN      
        1 cgccttcgcg gacgcgtcgt cccgtgcgca cgcaacgcac gcgttcgccg tcgatcacgc
       61 gcacttcgac ttgtccggtc tcgagtcgcc gctgaatctc cgaggccagc ttgcgagaac
      121 tgtaatggcc gccgtcgctg aaaaaaccgt catacaccgc ccaatcgaaa tcgaagtcca
      181 tcggcacgcg gacgcgcacc gcttcgctgc cgaccagcaa cgtgtattcg cgcaacgtcg
      241 ttggtagcac gagccacgaa cgcccgcgca cgatgcgcga atgcacgatg cctcggctct
      301 cgatgccgcc caccgggatc aacacttcgc cactcgcggg cgcgtcgagt cgatgcggcc
      361 aggcgccgaa cgccaataac cggcccgctt gttccaattt gttgggttcg tccgccggac
      421 tcgtgaagac ctccggcgtc atgccaaaat acgtcggcca catcgaattc gtcgggatct
      481 tgaacggctg cacgaaatac gtgcggatgc ccaggatcac gatcgccgcc accagaaaaa
      541 attccacgtt ctcgaccagg gaagtcttgg gataaatcgc gccgcccgtg cgccgcaaca
      601 ccgcttcgag cgactcgatc gagagcttca accgcgcagc gtcggccttg gctttgagct
      661 gttcgcgaag ctcggccgcg cgtttcaaca gctcggccga ttccttgtcc gacagcaaat
      721 cgcgccgaaa atgccacacc ttgtcggcga gctcgagcca gttgcccgca ttttcgcgcg
      781 ctttcttttc ctgggatgcg aagaggccga acatgtgtgg cgcgcgctta gttggtcttg
      841 agcaccttga taaatgcgtc cgacggaatc gagacacggc cgaactgctt catcttcttt
      901 ttgccctctt tctgcttgtc gaggagcttg cgcttgcggc tgatgtcgcc gccgtaacat
      961 ttcgatgtca cgtccttgcg catctccttc acgttgtcgc gcgcgatgac cttgccgccg
     1021 atcgccgcct gaatcgcgac cttgaacatc tgcggcggaa tgatctccgc gagcttctcg
     1081 caaagctcgc gccccttccc ttccgctttc tcgcgatgca cgatgctggc aaacgcgtcc
     1141 accggatcgc cgttgatgag aatttccatc ttcaccagat ccgacgtgcg atactcgccg
     1201 agttcgtaat ccatcgatcc ataaccgtgc gtgatgcttt tcagccggtc gttgaaatcg
     1261 acgaggattt cgttgagcgg gagcaggcag gtgagcatga cgcgattcgc atcgagcgtg
     1321 tccgtgtgat cgaccacgcc gcgtttttcc atcacgagag caaggatgtc gcccatcgcg
     1381 tcgttcggga tgtggatggc cgccttg
//
LOCUS       DASPLT010000635         1818 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_90121, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000635 DASPLT010000000
VERSION     DASPLT010000635.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1818)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1818)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1818
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_90121"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..930)
                     /locus_tag="VEA63_06585"
     CDS             complement(<1..930)
                     /locus_tag="VEA63_06585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0009678 - diphosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proton-translocating pyrophosphatase"
                     /protein_id="HYD83698.1"
                     /translation="MSDARASEANIQIPDLTQVSFDGLGGTSGLTLMYAGLALCLLGG
                     IFGMIQYWQTKALPVHASMAKVSHTIWETCKTYLFTQGKFLAILWLLIAACIFYYFSV
                     LQHNSLGHVLVILLASILGILGSYGVAWFGIRINTVANSRTAFSALKGNSLATLGIPL
                     RSGMSVGLLLVCVELFFMICILVFLPRDLVGPCFIGFAIGESLGASVLRICGGIFTKI
                     ADIGSDLMKIVFKLPEDDPKNPGVIADCTGDNAGDSVGPTADGFETYGVTGVALITFI
                     LLAVREPVVQVQLLVWIFSMRVMMVLASAVGYFV"
     gene            complement(1343..>1818)
                     /locus_tag="VEA63_06590"
     CDS             complement(1343..>1818)
                     /locus_tag="VEA63_06590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LysR family transcriptional regulator
                     substrate-binding protein"
                     /protein_id="HYD83699.1"
                     /translation="TEFEFVPLFTDELRFVTSPLHAWARDGRMTRDTLESETLVLYNK
                     SSYTARLVNEYFRDDRVVFGHYIELGSMDAIKELVKIGVGVGVLAPWIARAELEAGAL
                     VDFPLGRRRLRRTWGVAHLRGRRLPLGEETFIGLCRSVTEKFGEPSASPDPASAS"
BASE COUNT          372 a          549 c          592 g          305 t
ORIGIN      
        1 cacgaagtag ccgacagccg acgcgagaac catcatcacg cgcatcgaga agatccagac
       61 caggagctga acctggacca ccggctcgcg aaccgcgagc aggatgaagg tgatgagcgc
      121 gacaccggtg acgccgtagg tttcgaaacc gtccgccgtc gggccgacgc tgtcgccggc
      181 gttgtcaccg gtgcagtcgg cgatcacgcc ggggttcttc gggtcgtctt cggggagctt
      241 gaatacgatc ttcatcagat cggagccgat gtcggcgatc ttggtgaaaa tgccgccgca
      301 gatacgcagc accgacgcgc cgagtgattc gccgatcgcg aagccgatga agcacggacc
      361 gaccagatcg cgcggcagaa acaccaggat gcagatcatg aagaacaatt ccacgcacac
      421 gagcagcaag ccgacgctca tgccggagcg cagcggaatg ccgagggtgg cgagcgagtt
      481 gcccttcaat gcggaaaacg ccgtgcgcga gttggccacg gtgttgatcc ggatgccgaa
      541 ccacgcgaca ccgtaggaac cgaggatgcc gaggatcgag gcgagcagga tgaccagcac
      601 gtgacccagg ctgttgtgct gaagcacgct gaaatagtaa aagatgcacg cggcgatcag
      661 cagccagagg atcgcgagaa attttccctg cgtgaagagg taagttttgc acgtttccca
      721 aatcgtgtgc gacaccttcg ccatcgacgc gtgcaccggc agcgccttgg tctgccagta
      781 ttggatcatt ccgaaaattc cgccgaggag gcagagcgcc aaaccggcat acatcagggt
      841 caatccgctc gtgccgccga gcccgtcgaa actgacctgc gtgaggtcag ggatttgtat
      901 attagcttcg ctcgcgcgag catcggacat cactgacgcg gccaccgcgg ccgcgcccaa
      961 cttcaagcat tgaggtagct tcatagaggg gtaggagtga aaagaaccga ccggtttgaa
     1021 acgcagtggt tctgcgtccc gtgtgcgatg ttcgcgagcc tttagtcatt tcggagccgg
     1081 cgggaggtct ccctgattca ggccggtaaa accctgatgc gatggaggac gcgcgtgtcg
     1141 gtgcgaggtg cgtgtggtgt cggcgcgtcg taggttcacc gcatcgtcac cgatgcggct
     1201 acgcggcggg gtgggtagcg gcaggcctct gtgcctgcct gccggtggta ggttgcgcgc
     1261 ttgcgcgcaa cggggtgcga cgagcgcacc ccctgccgtt ccgaaccgat gagcaggcag
     1321 gcgcggaggc ctgccgctac ggtcaggaag cgctggcggg atcgggcgat gcggagggct
     1381 cgccgaattt ttcggtcacc gaccgacaga ggccgatgaa cgtttcttcg ccaagcggca
     1441 gtcggcggcc gcgcaaatgt gccacgcccc acgtgcgacg gagccgccgc cgccccagcg
     1501 gaaaatccac cagtgcgccg gcttcgagtt ccgcgcgcgc gatccacggc gcgagcacgc
     1561 cgacaccgac gccgattttc accagctcct tgatcgcgtc catgctgccc agctcgatgt
     1621 agtggccgaa caccacgcgg tcgtcgcgga aatattcgtt caccaaacgc gccgtataac
     1681 tcgatttgtt atagagcacg agcgtctcgc tttcgagcgt gtcgcgcgtc atccggccgt
     1741 cgcgcgccca tgcgtggagc ggcgacgtca cgaaacgcaa ctcgtcggtg aacagcggca
     1801 cgaattcgaa ctcggtcg
//
LOCUS       DASPLT010000636         2204 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_47162, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000636 DASPLT010000000
VERSION     DASPLT010000636.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 2204)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2204)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2204
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_47162"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..406
                     /locus_tag="VEA63_06595"
     CDS             <1..406
                     /locus_tag="VEA63_06595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD83700.1"
                     /translation="GAAEGGGGMIPMIDPVGIVGVIGMVIPMAVLFSAVIFTVALFAK
                     SYKEAQSYVTPLMIVVIMPAVVGMLPGIDLNATLALVPILNLSLVCKEMLSGVWHWNY
                     IALIFGSSCVYAAVALWLAVKMFQRESVMFRT"
     gene            752..1312
                     /locus_tag="VEA63_06600"
     CDS             752..1312
                     /locus_tag="VEA63_06600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD83701.1"
                     /translation="MNTSAPFPGSSPATKLPRRPVAGPSSGPGFSAEMSGQLAADLDA
                     LREREMNLRDYESRLRNWQSQLDAALVKQPSATAGSGSPFPRAGSGSPFASDTSLEAA
                     WEKFHRAHALLEAEQHQMRDDRIVLREMELALKRREAELAQREEIVAERERLLAEALE
                     RSPASTVQRLTQAPFVAAKAIFSSRK"
     gene            1752..>2204
                     /locus_tag="VEA63_06605"
     CDS             1752..>2204
                     /locus_tag="VEA63_06605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007360996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD83702.1"
                     /translation="MPVLTEPPPTRSSSRQQLELPSVSFFGRSLEEYTRFFSLDPLAL
                     RGRAVLDVAAGPSSFTAEACRRGIDAVAVDPLYGSTPDALATHVQLDYARMFAQMRAK
                     PRLFKVGRGNGRAFFSSIDEAEVDRRAAAQRFLTDYEAHFAHGRYVGAS"
BASE COUNT          385 a          645 c          739 g          435 t
ORIGIN      
        1 gggcgcggcg gagggcggag gcgggatgat cccgatgatc gacccggtgg gaatcgtcgg
       61 cgtaattgga atggtgatac cgatggcggt gttgttttcc gcggtgattt tcaccgtcgc
      121 gctgttcgcg aagagctaca aggaggcgca gagctacgtc acgccgttga tgatcgtggt
      181 gatcatgccg gcggtggtcg gcatgttgcc gggcatcgat ctcaatgcga cgctggcgct
      241 ggtgccgatt ctcaaccttt cgctcgtttg caaggagatg ctgtcgggcg tgtggcattg
      301 gaactacatt gcgctgatct tcggctcgtc gtgcgtgtat gcggcggtgg cgttgtggct
      361 ggcggtgaag atgtttcaac gcgagagcgt gatgtttcgc acgtgaggcg aacgactgag
      421 ccggaaaaat tcggttcaac cacgaatcga cacgaatgaa atcgagttcg gatgcggaat
      481 cggatcggaa caccaaacgg tgatacacac gatcggtccc aaagctttcc tctgcctcct
      541 ctgagttcgt gttcattcgt gtccattcgt ggtttcgcaa ctgcacgaat ccgactgagc
      601 gggcgtcccg gccgcggcgt aaaggacatc ggaaaaatta ccggggctgg gtaaagctcg
      661 ggtaaaaacc ggcgcagccc ataggcagcg gaggcggaat gtgattagga tgcgggcgtc
      721 gcccattgcg ctcgttttgc ggcttcacat catgaacaca tccgctcctt ttcctggcag
      781 ttctccggca acgaaacttc cgcggcgtcc ggtcgccggc ccttccagcg gtcccggatt
      841 cagtgctgaa atgagcggac agctcgccgc cgatctcgac gcgctgcgcg agcgcgagat
      901 gaatctgcgc gactacgaat cacgtctgcg aaactggcag tcgcagttgg acgcggcgct
      961 ggtgaagcag ccgagcgcga cggctgggag tggctcgcct tttccgcgcg cgggcagcgg
     1021 ttcgcctttt gccagcgaca cttcgctcga agcggcgtgg gaaaaatttc atcgggcgca
     1081 cgcgttgctc gaagcggagc agcatcagat gcgcgacgat cgcatcgtgc tccgcgagat
     1141 ggaactcgcg ttgaagcgtc gcgaggccga actcgcgcaa cgtgaggaga tcgtggcgga
     1201 gcgtgaacgt ctgctcgcgg aagcgctcga gcgatcaccg gcgtcgacgg tgcagcggtt
     1261 gacgcaggcg ccgtttgtgg ccgcgaaggc aatcttcagt tcgcgaaagt agggtggctg
     1321 gagcgtcgca gtctgcgtcg cgaatcggat cgtagcggca ggcctccgcg cctgactgct
     1381 tggtttccgg aaagggtggg tggtgcgctt gcgcaccacg ttgcgcgcga gcgcgcaacc
     1441 tacacaagca ggcaggcgcg gaggcctgcc cctacatcgc cgaatttgcg cttgcgcggc
     1501 aagcgacgcg tggtcatcac gacgcgcaat gaatctcgtc gtcaacgccc gcgtcgccgc
     1561 gtccgccatt gtaatggcgg cgggtaaacg cgtccgctgg cacgggtggg cccggcgacg
     1621 atggtgatgc gcgcaacgcg caacgatggc caaggagccg tctgaaatca gccaatcgcc
     1681 gagcccgact caaccgagtc gggctttttg tttttccccc tgcaaccacc gacctcaatc
     1741 cgtcccccgt catgcctgtc ctcactgaac ctcctccgac tagaagtagt agtagacaac
     1801 aactcgaact gccctcggtg tcgtttttcg ggcgttcgct cgaagaatac acgcgtttct
     1861 tttcgctcga tccactggcg ctgcgcggcc gcgcggtgct cgacgttgcg gcgggcccct
     1921 cgtcgttcac ggccgaggcg tgccgccgcg gcatcgatgc ggtggcggtc gatccgcttt
     1981 atggctcaac gcccgatgcg ctcgcgacgc acgtgcagct cgattacgcg cggatgtttg
     2041 cgcagatgcg cgcgaagccg cggctgttca aggttggccg gggcaacggc cgtgcgttct
     2101 tctcttcgat cgacgaggcg gaggtggacc ggcgggcggc ggcgcagcgg tttctcacgg
     2161 attacgaggc gcacttcgcg cacggacgct acgtcggcgc gtcg
//
LOCUS       DASPLT010000637         2370 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_90150, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000637 DASPLT010000000
VERSION     DASPLT010000637.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 2370)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2370)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2370
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_90150"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..207
                     /locus_tag="VEA63_06610"
     CDS             <1..207
                     /locus_tag="VEA63_06610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HYD83703.1"
                     /translation="ERPAQLSLRLAQMNELGVFEAVHDAVQAEIGAACAALQEWPGEP
                     PTPLLLSLCDVLRGQLAALRPARG"
     gene            392..1027
                     /locus_tag="VEA63_06615"
     CDS             392..1027
                     /locus_tag="VEA63_06615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HYD83704.1"
                     /translation="MDAAKILGKTLVASPDRQQEAGSDMSIVRQVQSGDVAAFDQLVV
                     KYRERVYSVVYNLTSNREDAADLTQDAFIKAFQSINRFQGQSSFFTWLYRIAVNSTLT
                     HLRKHRLRTFFSFEKIDESDPISADVLAALTDATGADRETFARELQEKLNEAMQKLSI
                     KHRTVITLFEIDGLSHQEIAEVMDCSVGTVRSRLHYAKQLLQSELQPYTRR"
     gene            1024..1845
                     /locus_tag="VEA63_06620"
     CDS             1024..1845
                     /locus_tag="VEA63_06620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD83705.1"
                     /translation="MTSKKNTVSVEDLLRLKRAERPRPEFWAEFERELRVKQLAAIVE
                     PRPWWSPFIRVSARVARLQLPMGAAAVLAISFVTVREYREPDAESLRLPEIAAPAVYS
                     EAMTRVAITPSGSDLATRAPAEAQLAEATSAESQPTDAMAAMEVGQAAHVVALLDNNT
                     SLHREPTPSARYIAANLAAAQADNPGLMDDVFGAATSSRAKLREPVRDPLAQMAVPGE
                     SRRSRLLATALPAVVGAADMEITGGDRMSRRLTEDRLYDSISRIGVKGDRVAFKF"
     gene            1937..>2370
                     /locus_tag="VEA63_06625"
     CDS             1937..>2370
                     /locus_tag="VEA63_06625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lyase family protein"
                     /protein_id="HYD83706.1"
                     /translation="MRTEKDSMGEMAVPDDALYGASTQRAVLNFPISGRPLPEPFIRG
                     LGLVKLACARANLELGLLSPEKSQLIQTVAREIADGRLSSHFPIDVFQTGSGTSTNMN
                     ANEVIANRASRISGLPIGAKKPLHPNDEVNLGQSSNDIIPTTL"
BASE COUNT          421 a          704 c          773 g          472 t
ORIGIN      
        1 gagcggcccg cgcagctttc actgcggctc gcgcagatga acgagctcgg ggtgttcgaa
       61 gcggtgcacg acgcggttca ggccgagatc ggcgcggcct gtgcggcgtt gcaggaatgg
      121 ccgggcgagc cgccgacgcc attgttgctg agtctttgcg atgtgttgcg cgggcagctc
      181 gcggcgctgc ggccagcgcg aggataggcc ccgacgttct cgtcggactt caaaacgttt
      241 cgcgcgcaac cagcctgacg aggacgttgg gctccacctg ctactgcatg agcctctccc
      301 gcagcgcggg cgatgaaatg ggcgtaactt tgtcggagct gcccactttc catttgcgcc
      361 gccgcggtgc ctcggctttg ttcggttcgc aatggatgcc gccaaaatcc tcggcaagac
      421 gctcgtggcg tcccccgacc gccagcagga ggcgggctcc gatatgtcga tcgtgcgcca
      481 agtgcagagc ggcgatgtcg cggcgtttga ccagctggtc gtgaagtatc gtgaacgcgt
      541 ttacagcgtg gtctacaact tgacgtcgaa tcgcgaggac gccgctgact tgacgcagga
      601 cgctttcatc aaggcctttc agtcgatcaa tcgtttccaa ggccagtcgt cgttcttcac
      661 ctggctttat cgcatcgcgg tgaattcgac gctcacgcat ttgcggaaac accggctgcg
      721 cacgttcttc agcttcgaga aaatcgacga gagcgatccg atctcggccg acgtgctggc
      781 ggcgctcacg gatgcgacgg gcgctgaccg ggaaaccttt gctcgcgagc tccaggaaaa
      841 attgaacgag gcgatgcaga aattgtctat caagcacaga accgtgatca cactttttga
      901 gatcgatggc ctcagccatc aggaaatcgc cgaggtaatg gattgctcgg tgggcacggt
      961 tcggtcgcgg cttcattatg ccaagcagct ccttcagtct gaattgcagc cttatactcg
     1021 ccgatgacgt ccaagaaaaa cactgtgtca gttgaggact tgctgcgcct gaagcgggct
     1081 gagcgcccgc gtccggaatt ctgggccgag ttcgagcgtg agctgcgcgt gaagcaactc
     1141 gccgcgatcg tcgagccgcg cccttggtgg tcgccattca ttcgggtgag cgcacgcgta
     1201 gcgcgtttgc aattgccgat gggtgccgcc gcggtgctcg cgatttcctt cgtcaccgtt
     1261 cgcgagtatc gcgagcccga tgccgagagc ttgcgtttgc cggagattgc ggctcccgcc
     1321 gtttattctg aagcgatgac gcgtgtggcg attacgccga gcgggtctga tctcgccacg
     1381 cgagcgcccg ccgaggcgca gctagcggaa gcgacttctg cggagtcgca gccgaccgat
     1441 gcgatggcgg cgatggaagt gggccaggcg gcgcacgtgg tggcgctttt ggacaacaat
     1501 acgtcgctgc accgcgagcc aacaccgtcg gcgcgttata tcgcggccaa ccttgccgcg
     1561 gcgcaggccg acaatcccgg tctgatggat gatgtttttg gcgccgcgac gagcagccgt
     1621 gcgaaactgc gcgaacccgt tcgcgatccg ttggcgcaga tggccgtgcc cggcgaatcg
     1681 cgtcgttcgc gtttgctggc cacggcgttg ccggcggtgg tcggtgcagc ggacatggag
     1741 atcacgggcg gcgatcgcat gtcgcgccgg ctcacggaag atcggctgta tgacagcatc
     1801 agtcggatcg gcgtgaaggg cgaccgggtt gctttcaaat tctaaccgct cgcgtcgcgc
     1861 ttgcggtttc gtttttaggc cccgctgcag gcggggcctt ttgctttccg cagatatgga
     1921 ttaacatcgg cgcatcatga gaaccgaaaa ggactcgatg ggcgaaatgg cggtgccgga
     1981 cgacgcgctg tatggcgctt ccacgcagcg ggctgtcctg aactttccca tcagtggacg
     2041 gcctctgccg gagccgttca ttcgcgggct cggactcgtg aagctcgcgt gcgcgcgcgc
     2101 gaacctcgag ctcgggctcc tctcgccgga gaaaagtcag ctgattcaaa ccgtggcgcg
     2161 cgagatcgcg gacgggcggc tctcgtcgca ttttccgatc gacgttttcc aaacgggctc
     2221 gggcacgtcg accaacatga acgcgaacga ggtgatcgcg aatcgcgcga gccggatctc
     2281 gggtttgcca atcggcgcaa aaaagccgct gcatccgaac gacgaggtga atcttggcca
     2341 gtcgtcgaac gacatcattc cgacgacgct
//
LOCUS       DASPLT010000638         1110 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_150921, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000638 DASPLT010000000
VERSION     DASPLT010000638.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1110)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1110)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1110
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_150921"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(232..>1110)
                     /gene="ruvB"
                     /locus_tag="VEA63_06630"
     CDS             complement(232..>1110)
                     /gene="ruvB"
                     /locus_tag="VEA63_06630"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HYD83707.1"
                     /translation="RGEPLNHILLSGPPGLGKTTLAFILGHELGKNVRVTSGPVVEKA
                     GDLAGLLTNLEEGDILFIDEIHRIPKTVEEYLYSAMEDFRLDIMIDQGPNARSVRLTI
                     PRFTLVGATTRAGLLTAPLRSRFTLQTRLDYYDRATLETIVRRSCALLKVPVDQGGAQ
                     EIASRARGTPRVANNLINFVRDYAQQRANGKITQPVAAAALELLEIDAAGLDEMDKRM
                     LRVMGENYGGRPVGMSTIAVAVGEEAETLEEVHEPFLIQEGYIQRTREGRILTPKGYA
                     AIGLKPFGGPEQPTLL"
BASE COUNT          195 a          292 c          405 g          218 t
ORIGIN      
        1 ggtgatgttc tgcacgcggc ggatcgattg gtatgcggtg cagttgggcg aggagaattc
       61 atctcaacca cgaatggaca cgaaaggaca cgaatgaaaa ccgagttcga aagcagcgct
      121 catcggaatg ccgaagcgtg agcgagcgaa acgttgaatc gtttgagcgc ggccaatggc
      181 cggagaatgc gtgagcgcaa tggcggacgg ctcggagagc cgtccctacc ttcagagcag
      241 cgtgggctgc tcggggccgc cgaagggttt taggccgatg gcggcgtagc ctttcggggt
      301 gaggatgcgg ccttcgcgcg tgcgttgaat gtagccttcc tggatcagaa aaggctcgtg
      361 cacttcctcg agcgtttcgg cttcctcgcc gacggcgacg gcgatggtgc tcatgccgac
      421 gggacggccg ccgtagtttt cgcccatgac gcggagcatg cgtttgtcca tttcgtcgag
      481 gccggcggca tcgatttcaa ggagttcgag agcggcggcg gcgacgggtt gcgtgatctt
      541 gccgttggct cgctgctggg cgtagtcgcg cacgaaattg atcaggttgt tggcgacgcg
      601 cggcgtgccg cgagcgcgtg acgcgatttc ctgagcgccg ccctgatcga ctggcacttt
      661 cagcaacgcg cagcttcggc gcacgatcgt ttcgagcgtg gcgcgatcgt agtagtcgag
      721 gcgcgtttgc agcgtgaaac gcgaacgcag cggtgcggtg agcaggccgg cgcgcgtggt
      781 ggcgccgacg agtgtgaaac gcggaatcgt gagccgcacg ctgcgggcgt tgggcccctg
      841 atcgatcatg atgtccaacc ggaagtcctc catcgccgag tagagatact cttcgacggt
      901 cttggggatg cggtggattt cgtcgatgaa gaggatgtcg ccctcttcga gattcgtgag
      961 caggccggcg aggtcaccgg ctttctcgac gactgggccg gatgtgacgc ggacattttt
     1021 cccgagctcg tggccgagga tgaaggcgag cgtggttttg ccgaggccgg gcggaccgga
     1081 gagcaggatg tgattgagtg gctcaccgcg
//
LOCUS       DASPLT010000639         1081 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_31478, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000639 DASPLT010000000
VERSION     DASPLT010000639.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1081)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1081)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1081
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_31478"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..383)
                     /locus_tag="VEA63_06635"
     CDS             complement(<1..383)
                     /locus_tag="VEA63_06635"
                     /inference="COORDINATES: protein motif:HMM:NF024742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HYD83708.1"
                     /translation="MSGKELCAYGTGIIAYQFVHSGIAALAMVIFNIELGLAPGLVGA
                     VLMAGRIWDAITNPIVGALSDNTRTRWGRRRPYLLAGAIACGVIYPLVWLAPREASEN
                     LLLGYLLLTSLLLYTAFAFYSVPYMA"
     gene            complement(461..>1081)
                     /locus_tag="VEA63_06640"
     CDS             complement(461..>1081)
                     /locus_tag="VEA63_06640"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HYD83709.1"
                     /translation="EEVGVRMNFLGDRLSVEASAFDITNTNVVQGDPFGNVGVFPPDP
                     RNDWKFVDGISNRGFEVDVKVNLSGATQVLFSYANYDLKNAGNPAQVAAGIKEYPVNN
                     SPQHQASLWLKWSPQHEALRGWRLTGGARYMGERSAGGIGALPSLYLPAYTIVDAGVG
                     YAREKWAVDLLVRNVFDEYAIRTASTNNRIYPEQPLHAMLAASVSF"
BASE COUNT          226 a          333 c          332 g          190 t
ORIGIN      
        1 gccatgtaag gcaccgagta gaaagcgaag gcggtgtaga gcagcagtga tgtcagcagt
       61 agatatccga gcagcaggtt ttccgacgcc tctcgcggtg cgagccacac gagcggataa
      121 atcacgccgc aggcgatcgc cccggcgagg agatagggcc ggcgacgacc ccagcgcgtg
      181 cgggtattat ccgacagcgc gccgacaatg ggattggtga tggcgtccca gattcgtccg
      241 gccatcaaca ccgcgcctac caatcccggc gccagcccga gctcgatatt gaagatcacc
      301 atcgccagcg cggcgatgcc gctatggaca aactgatacg cgatgattcc agtgccgtag
      361 gcgcacagct cctttccgct caaacggtca cgtgtgtctg ctggagtcaa aggcaggccg
      421 tgcccgcgag accgcgggca cgtgtcgatg tttagggggc tcagaaactc acactggcgg
      481 cgagcatcgc atgcaacggc tgctcgggat aaatgcgatt gttggtcgaa gccgtgcgga
      541 tggcgtattc atcgaagacg tttcgcacca gcagatcaac cgcccacttc tcccgcgcat
      601 atcccacgcc cgcgtcgacg atcgtgtagg cgggcaggta aagcgacggg agcgcgccga
      661 tgccccccgc cgatcgttcg cccatgtagc gcgcaccgcc cgtcaaccgc cagccgcgca
      721 gtgcctcgtg ctgagggctc cacttgagcc aaagcgacgc ctggtgctga ggagagttgt
      781 tcactggata ctccttgatg ccggcggcga cttgcgcggg attaccggcg ttcttcaaat
      841 cgtagttggc atacgagaag agcacctggg tcgcaccgct gaggttgacc ttcacatcga
      901 cctcgaagcc gcgattgctg atgccgtcga cgaacttcca atcgtttcgc ggatcaggcg
      961 ggaaaacacc gacattaccg aaggggtcgc cctgcacgac attcgtgttg gtgatatcga
     1021 acgcggacgc ttcaaccgag aggcgatcgc cgaggaaatt catgcgcacg ccgacttcct
     1081 c
//
LOCUS       DASPLT010000640         2982 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_294872, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000640 DASPLT010000000
VERSION     DASPLT010000640.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 2982)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2982)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2982
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_294872"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..583
                     /locus_tag="VEA63_06645"
     CDS             <1..583
                     /locus_tag="VEA63_06645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glutamate synthase subunit alpha"
                     /protein_id="HYD83710.1"
                     /translation="YPPANATFKPQDNIIVGNVAFYGATAGEIYLNGRAGERFAVRNS
                     GVNAVVEGVGDHACEYMTGGRVVILGSTGRNFAAGMSGGVAYVLDESGAFTKDVNLAM
                     VGIEKLDSPAEIAEVRLLIENHHTYTKSARAKEVLDAWDATVPKFVKVLPKDYKRMLA
                     CIERAQSQGLTGDEAIMAAFEENSRDTSRVGGN"
     gene            627..2111
                     /locus_tag="VEA63_06650"
     CDS             627..2111
                     /locus_tag="VEA63_06650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009510296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit beta"
                     /protein_id="HYD83711.1"
                     /translation="MGKPTGFIEYLRELPVDRAPLERVRDWKEFHHHMEEKKLRTQGA
                     RCMDCGVPFCHTGKLISGMASGCPINNLIPEWNDLVYRGLWKEALDRLHKTNNFPEFT
                     GRVCPAPCEGSCVLGINNPPVTIKNIEAAITDRGWAEGWVNPEQPKIRTGKKVAVIGS
                     GPAGLSAAEQLNKAGHTVTVFERADRPGGLLMYGIPNMKLDKKEVVLRRIKLMEQCGI
                     KFICNANVGENVEPQMFLKEYDATVICTGATKPRDLPIEGRSLKGVHFAMEFLTENTK
                     ALLNGGADHCPIHAKGKDVVVIGGGDTGTDCVGTSMRHGAKSITQIEILPKPPMDRAA
                     DNPWPEWPKVYKMDYGQEEAAAKFGADPRVYLTTVKKFIGDENGAVKELVTVQITWGK
                     NDKGQFVPTEVPGTEQTRPAQLVLLAMGFLGPEQAFLKEINLETDARSNIKAEHEKYT
                     TNVKGVFAAGDCRRGQSLVVWAINEGRGAARECDRYLMGSTDLP"
     gene            complement(2330..>2982)
                     /locus_tag="VEA63_06655"
     CDS             complement(2330..>2982)
                     /locus_tag="VEA63_06655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HYD83712.1"
                     /translation="GVMDQGEAGRYRTIAVRVGRHVPPAPDMVSGLMRELLDWWNGPS
                     VEWSPVLTSAILHYQFEDIHPFGDGNGRTGRLLALWELYRRGFDTHHVFSVDEFFWED
                     RERYYASLAIVPKQGGNLTSWLEYTTEGVRRTLERVWLRVQQLAAKSGSKKMVLRPKQ
                     EQLLHLLRERRELAPRDIWAALGVTKQGAAHILAPLLKAKLVKRVGTRKTGRYVLT"
BASE COUNT          611 a         1001 c          862 g          508 t
ORIGIN      
        1 ctacccgccg gcgaacgcga cgttcaagcc gcaggacaac atcatcgtcg gcaacgtcgc
       61 gttctacggc gcgaccgccg gcgagattta tctcaacggc cgcgccggcg agcgcttcgc
      121 cgtccgcaac tccggcgtca acgccgtcgt tgaaggcgtg ggcgaccacg cttgcgaata
      181 catgacgggc ggccgcgtcg tcatcctcgg ctccaccggg cgcaatttcg ccgccggcat
      241 gtcgggcggc gtcgcctacg tgctcgacga gagcggcgcc ttcacgaagg acgtgaacct
      301 cgcgatggtc ggtatcgaga aactggattc gcccgccgag atcgccgaag tccgtctgct
      361 catcgagaac caccacacct acacgaagag cgcccgcgcg aaagaagtgc tcgatgcgtg
      421 ggacgcgacc gtgccgaagt tcgtgaaagt gcttccgaag gactacaagc gcatgctcgc
      481 ctgcatcgag cgcgcgcagt cccaaggact caccggcgac gaagccatca tggccgcctt
      541 cgaagaaaac tcccgcgaca cctcgcgcgt cggcggaaac taaccgggct ctcagctctc
      601 aactctaagc tctcagctaa tttcccatgg gcaaacccac cggcttcatc gaatacctcc
      661 gcgaacttcc cgttgatcgc gcgccgctcg agcgcgtgcg cgattggaag gagtttcacc
      721 accacatgga ggagaagaaa ctccgcacgc agggcgcgcg ctgcatggat tgcggcgtcc
      781 cgttctgcca caccgggaag ctcatcagcg gcatggcttc gggctgtcca atcaacaacc
      841 tgatcccgga gtggaacgac ctcgtttacc gcggcctctg gaaggaagcg ttggatcgcc
      901 tgcacaagac gaacaacttt cccgagttca ccggccgcgt gtgcccggcg ccttgcgaag
      961 gttcgtgcgt tctcggtatc aacaacccgc ccgtcacgat caagaatatc gaggcggcga
     1021 tcaccgaccg cggttgggcg gagggctggg taaatcccga gcagcccaag atccgcaccg
     1081 gcaagaaagt cgcggtcatc ggctccggcc ccgcgggcct ctccgccgcc gagcagctca
     1141 acaaagccgg tcacaccgtc acggtgttcg aacgcgctga tcgtcccggc ggcctgctca
     1201 tgtatggcat ccccaacatg aagttggaca agaaggaagt cgtccttcgc cgcatcaagt
     1261 tgatggagca gtgcggcatc aaattcatct gcaacgccaa cgtcggcgaa aatgtcgagc
     1321 cgcagatgtt cctgaaggaa tacgacgcga ccgtcatctg caccggggcg accaagccgc
     1381 gcgatctccc gatcgaaggc cgcagcctca agggcgtgca cttcgcgatg gagttcctca
     1441 ccgaaaatac gaaggcgctt ctcaacggcg gcgccgacca ctgcccgatc cacgcaaagg
     1501 gcaaggatgt cgttgtcatc ggcggtggcg acaccggcac ggactgcgtc ggcacttcga
     1561 tgcgccacgg cgcgaagagc attacgcaaa tcgaaattct ccccaagccg ccgatggatc
     1621 gcgcggccga caatccgtgg cccgagtggc cgaaggtcta caagatggac tatggccagg
     1681 aggaagccgc cgcgaaattc ggtgccgatc cccgcgtgta tctcacgacg gtgaagaaat
     1741 tcatcggcga cgaaaacggc gcggtgaagg aactcgtcac tgttcagatc acctggggca
     1801 aaaacgacaa gggccagttc gtgccgacgg aagtccccgg caccgagcag acccgccccg
     1861 cgcaactcgt gttgctcgcg atgggttttc tcgggccgga gcaggcgttt ctcaaggaga
     1921 tcaacctcga gaccgacgcg cgttcgaaca tcaaggccga gcacgagaaa tacacgacga
     1981 acgtcaaagg cgtcttcgcc gccggtgact gccgtcgtgg ccagagcttg gtcgtctggg
     2041 ccatcaacga aggccgcggc gccgctcgcg agtgcgaccg ctacctcatg ggcagcaccg
     2101 atctcccgta attattcaag acacccaaaa ggtgaagccc ggcgtccccc gccgggcttt
     2161 ttttggggta gggcgaggcg tccccgccga gccgcgcaat gcttcgtctg cgttcttcct
     2221 cttgagcagt taaagcagaa gcgtagcgga gatcgccttg tgaaaggggc tttatgcccg
     2281 actcattggc gcgttcgtcg gggcatgaag accctcccac cgttacgccc tacgtcagca
     2341 catagcgccc tgttttcctc gtgcccactc gtttcacgag ctttgccttc aacaacggcg
     2401 cgagaatatg cgccgcccct tgcttcgtta caccgagcgc tgcccaaatg tccctcggcg
     2461 cgagctcccg ccgctcgcgc agcaagtgaa gcaactgttc ctgcttcggc cgcagcacca
     2521 tttttttcga tcccgatttg gccgcgagtt gctgcacgcg cagccacacg cgttccaacg
     2581 tccgtcgcac tccctccgtc gtgtattcca gccagctcgt caggttgccg ccctgtttag
     2641 gcacgatcgc aagactcgca tagtaacgct cgcgatcctc ccaaaaaaat tcgtcgaccg
     2701 agaacacgtg gtgcgtgtcg aagccgcgcc gatacaactc ccacaacgcc agcagccgac
     2761 ctgtgcgacc gttgccgtcc ccgaacgggt gaatgtcttc gaactgataa tgcagaatcg
     2821 cggacgtgag caccggcgac cactccacgc tcgggccgtt ccaccaatcc agcaactcgc
     2881 gcatcagccc tgaaaccatg tccggcgccg gcgggacgtg ccgaccgacc cgcaccgcga
     2941 tcgtgcgata gcgtccggcc tcgccttggt ccatgacgcc ac
//
LOCUS       DASPLT010000641         1279 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_232727, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000641 DASPLT010000000
VERSION     DASPLT010000641.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1279)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1279)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1279
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_232727"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..386
                     /locus_tag="VEA63_06660"
     CDS             <1..386
                     /locus_tag="VEA63_06660"
                     /inference="COORDINATES: protein motif:HMM:NF012645.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP synthase F0 subunit B"
                     /protein_id="HYD83713.1"
                     /translation="MEERNRKIESGIKHAEEMQAKLAAAQQESATIVKKAQLEASRIV
                     DDARKTAKEYLDKQTQEASTKANDLLTKAQQAIELEHKKMLADARGEIARLVIVTTER
                     VLAKKLTDADRASYNEAAARELTSV"
     gene            438..833
                     /locus_tag="VEA63_06665"
     CDS             438..833
                     /locus_tag="VEA63_06665"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HYD83714.1"
                     /translation="MASAKKQTQQLARQFYKLSFENGVLSADRVAGVLAYIEKHRPAH
                     TLAVLHAYQRLIAAEVARSQAVVEHAGPASNAALDAIAAALSKKYHRPVTAVAKRNDA
                     LLAGLRVRVGDDTYESSVAGQLAALSAAV"
     gene            883..>1279
                     /locus_tag="VEA63_06670"
     CDS             883..>1279
                     /locus_tag="VEA63_06670"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373703.1"
                     /note="produces ATP from ADP in the presence of a proton
                     gradient across the membrane; the alpha chain is a
                     catalytic subunit; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HYD83715.1"
                     /translation="MSTVLEQIEQQIAKLSTKAVKKNTGHIRTVADGVAKIDGLSDVM
                     YNEMVQFPGGAIGIALNLEEDEVGCVVLGDISKLKEGDEVQTTGRLLSVPVGKALLGR
                     AVDALGNPVDGKGPIAATETNPVEKIAPGI"
BASE COUNT          282 a          461 c          342 g          194 t
ORIGIN      
        1 cgatggagga gcgcaaccgg aagatcgaat ccggtatcaa gcacgccgaa gaaatgcagg
       61 ccaagctcgc ggccgctcaa caggagagcg ccacaatcgt gaaaaaggcc cagctcgagg
      121 cttcccgcat cgtcgacgac gcccgtaaaa cggcgaaaga gtatctcgac aaacaaacgc
      181 aggaagcttc gaccaaggcg aacgatctcc tcacgaaggc gcagcaggcg atcgagctcg
      241 agcacaagaa aatgctcgcc gacgcccgcg gcgaaatcgc ccgcctcgtc atcgtcacga
      301 ccgagcgcgt cctcgccaag aaactcaccg acgccgatcg cgcttcctac aacgaagccg
      361 ccgcgcgcga gctcacctcg gtttgatcgc gccgtccgct cttagcttcc gacccttaag
      421 cttctcgctc caccgcgatg gcctccgcca aaaaacaaac tcaacagctc gcccgccagt
      481 tctataaact gagcttcgaa aacggcgtcc tgtccgccga tcgcgtcgct ggcgtcctcg
      541 cctatatcga gaagcaccgc ccggctcaca cgctcgcagt gcttcacgcc taccaacgct
      601 tgatcgccgc cgaggttgcg cgcagccagg ccgtcgtcga gcacgccggc ccggccagca
      661 atgccgccct cgacgcgatc gccgccgcac tctcgaaaaa ataccaccgt ccggtcacgg
      721 cggtcgcgaa gcgcaacgac gcgttgctcg ccggtctgcg cgtccgcgtg ggcgacgaca
      781 cctacgagtc ctccgtcgcc ggccagctcg ccgctttgag cgctgccgtc tgattctccc
      841 cgtcccgcgc cccttcatat ccgaatttca aatacatctc cgatgagcac cgtcctcgaa
      901 caaatcgaac aacagatcgc caagctctcg accaaggcgg tcaagaagaa caccggccac
      961 atccgcaccg tcgccgacgg cgtcgccaaa atcgacggcc tctccgacgt gatgtataac
     1021 gagatggtgc agttccccgg tggcgccatc ggcattgcgc tcaatctcga ggaagacgaa
     1081 gtcggctgcg tcgtgctcgg cgacatttcg aaattgaagg aaggcgacga agtccagacc
     1141 accggacgcc ttctctccgt gcccgttggc aaagcccttc tcggccgcgc ggtcgatgcc
     1201 ctcggcaatc ccgtcgacgg caaaggcccg atcgcggcga ctgagacgaa ccccgtcgaa
     1261 aaaatcgcgc ccggcatca
//
LOCUS       DASPLT010000642         1709 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_253596, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000642 DASPLT010000000
VERSION     DASPLT010000642.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1709)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1709)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1709
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_253596"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1709)
                     /locus_tag="VEA63_06675"
     CDS             complement(<1..>1709)
                     /locus_tag="VEA63_06675"
                     /inference="COORDINATES: protein motif:HMM:NF024603.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IPT/TIG domain-containing protein"
                     /protein_id="HYD83716.1"
                     /translation="DLLFGAYVYLEPFRVTSVQPNFGPTAGGTAVVIKGTGFAVDNDV
                     QVWFGAAPATGVQVLDSTSIRVTTPNGLLGPVDVVVKNDASKSNSATVTLAAGFLYAQ
                     RDDVTLATGLTVQDWAIEPASNLAFVIGTSAAEGSVLKVVNLTVDGEDLSQRVGLNQT
                     IATLKLPQEANGAIAILPERDLVFVSTKTDLIVIDAGGLDFESNPANELVVLATRSLD
                     STQGGGIAAAGNTVYVAAGDAGVRVFNTHFPDRPFLMDTIDTRDAGLDVDIIGSSALV
                     VREGVFAAPIAQRRYPYANGQVQRHSGSAEIFDIISSEHEPLSRIEIDFERMKAGGDA
                     LYFVSQLDGLAKYDVRHLRAPVLLGKLGGLGRLNDFDLRFALGFAAVGNTGMQYLDLS
                     PPNFADGSVLARATNPTFDRELLQVYQSFGVVYGLFANGEIQVLATRQMSPHSVFPEP
                     SSLVEAGLDQIKAVFISELAGASVTTANVRIVDETAETSVAFTDFDLIVEGNTILLNL
                     QSPLAPGHAYRVEIDRSIKDIFGLSPLADLVWRFRTATQAAAIRPEITEIYPGFAPTS
                     GGT"
BASE COUNT          348 a          544 c          512 g          305 t
ORIGIN      
        1 cgtcccaccg ctcgtcggcg cgaagccggg atagatctcc gttatctctg ggcggattgc
       61 agcggcctgc gtcgccgtcc ggaaacgcca cacgagatcg gcgagtggcg acaagccgaa
      121 gatgtccttg atcgaacgat caatctcgac gcgataggca tgtccgggcg ccagcggaga
      181 ctggagattg agcaggatgg tgttgccctc gacgatgaga tcgaagtcgg tgaacgcgac
      241 ggatgtttcg gccgtttcat ccacgattcg cacgttcgcg gtcgtcacac tcgcgccggc
      301 cagctcactg atgaaaacgg ccttgatctg atcgagccct gcctcgacca acgagctcgg
      361 ctcaggaaac accgagtgcg gactcatctg gcgcgtggcg agcacttgaa tctcgccgtt
      421 cgcaaacaac ccgtagacga cgccgaagga ttgataaact tgcagcagtt cccgatcgaa
      481 cgtcggattg gtggcgcggg ccaacaccga accgtccgca aagttcggcg gagaaagatc
      541 gagatactgc atgccggtgt tccccaccgc cgcgaagccg agcgcaaaac gaaggtcgaa
      601 gtcgttgagg cggccaagcc caccgagctt gccgagcaac acgggagccc gcaggtgcct
      661 cacgtcatac ttcgccagac cgtcgagctg cgaaacaaag tagagtgcgt caccccccgc
      721 cttcatgcgc tcgaagtcga tctcgattcg gctcagcggc tcgtgctcag aactgatgat
      781 gtcgaaaatc tccgccgagc cgctgtgacg ctgcacttgt ccgttggcat acgggtagcg
      841 acgctgcgcg atcggagctg cgaacacgcc ttcgcgaacc accagcgccg agctaccaat
      901 gatgtcgaca tccagcccgg catcgcgcgt atcgatcgta tccatcagga atggacgatc
      961 gggaaaatgc gtgttgaaca cacgcacacc cgcgtcaccc gccgccacgt agaccgtgtt
     1021 gcccgccgcc gcgatgccgc cgccctgagt gctgtcgagc gaacgcgtgg ccaggacaac
     1081 cagttcattc gcgggattgc tttcgaaatc caacccgccc gcatcgatga caatcaggtc
     1141 cgtcttcgtg ctcacgaaga ccaggtcgcg ctccggcaaa atcgcaatgg cgccgttggc
     1201 ttcctgtggc agtttgagcg tggcgatggt ttgattgagg ccgacgcgtt gagaaaggtc
     1261 ctccccgtcg accgtcaagt tgaccacctt caacacgctg ccttcggccg cgctcgtgcc
     1321 gatcacgaag gcgagattcg atgccggttc gatcgcccaa tcctgcaccg tcagtccagt
     1381 cgccagggtg acgtcgtcgc gctgcgcgta aagaaaaccg gccgccaacg tgaccgtggc
     1441 gctgttggat ttgctggcgt cgtttttgac cacgacgtcg accggaccga gcaggccgtt
     1501 tggcgtggtc acgcggatac tcgtgctgtc gagcacctgc acgccggttg caggcgccgc
     1561 gccaaaccag acttgaacgt cgttatcgac ggcaaatccg gtgcccttga tcaccactgc
     1621 cgtgccgccg gcggtcgggc cgaaattcgg ctgcacgctc gtgacgcgga acggctccag
     1681 atacacatag gcgccaaaca acaggtcgg
//
LOCUS       DASPLT010000643         2036 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_47274, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000643 DASPLT010000000
VERSION     DASPLT010000643.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 2036)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2036)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2036
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_47274"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            184..795
                     /locus_tag="VEA63_06680"
     CDS             184..795
                     /locus_tag="VEA63_06680"
                     /inference="COORDINATES: protein motif:HMM:NF019575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HYD83717.1"
                     /translation="MSSPSPRPSQPHRSRSRSRGMTLVEVMVAMLVLTTAMLGFLGSF
                     IQSRRITESSVLHAAASSLVYGLLEQMKGLDYSSVPSGSPPEIRLRVTPTDIVSLRAV
                     YTQAPNAPKAPATTPAFSVTAATLGAVDNIIGPLPLSSSSGTRSQQLRINLWVWIDEI
                     PDAARDVKEVKKITVVYSYDFNDGNRVRTVRDREVILRTRFDQ"
     gene            792..1535
                     /locus_tag="VEA63_06685"
     CDS             792..1535
                     /locus_tag="VEA63_06685"
                     /inference="COORDINATES: protein motif:HMM:NF019575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HYD83718.1"
                     /translation="MKTRPLRRRSNAGYTLTEIMITTTIFGMVVAGTLTVFMMGMRSM
                     YRDIERLSTDSTLRRLTLHVAKETIDSTEFYVFPNYQSLDGSVNLVTDISPLSPAEPD
                     EGETQLASGDCLVLVTRVTIESTSNVRQFRIYYRPVTSAGAQAPLRYYESTDYGSVGS
                     EDSLNALLNAVNLKTTPAYPGSRELASITRGKPIPSSTDHFPVFSTEATVPTATNESV
                     SLNIEVINGKSAVNMLSSSSFNYTISPRR"
     gene            1541..>2036
                     /locus_tag="VEA63_06690"
     CDS             1541..>2036
                     /locus_tag="VEA63_06690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD83719.1"
                     /translation="MKAPRSSRQRIQGYALLSVIVFVAIMALLTASMLKYSSSERRMN
                     ERSRLLLRARNMAENAALYASEQLTTKLYRLRSATPMAFMTGSNQIFLPPDEVLTTSF
                     SQPSDVEVRAGLTYSTDLEFIDPTEAENANNPNAGLSVSTSTVPIVAKATMRHPSLGE
                     VTAHV"
BASE COUNT          398 a          782 c          493 g          363 t
ORIGIN      
        1 gtttcgtctg ggcgagagcc gaaaagctca cgcttttcgc tacgggacga attttcccgc
       61 taatctcgac acccctccct catcgcccgc caccaaggcc attcggccct atcgcattag
      121 ggctctttca gcagtccgcc gcctcttact caagcgagcg gcatctgtta cgatagatgc
      181 gaaatgagtt cgccgtcacc tcgtccatcc cagccgcacc gatcgcgctc gcgctcccgc
      241 ggcatgactt tggtcgaagt gatggtcgcg atgctcgtgc tcaccaccgc catgctcggc
      301 ttcctcggga gcttcatcca gtcgcggcgt atcaccgaat ccagcgtcct gcacgccgcg
      361 gccagcagtc tcgtttacgg cctgctcgaa caaatgaaag gcctcgacta cagttcggtc
      421 ccaagcggct cgccgccgga aattcgcctg cgcgtgacgc cgaccgacat cgtctcgttg
      481 cgagccgtct acacgcaggc tcccaacgcc cccaaagcgc ccgccaccac ccccgctttt
      541 tccgtcaccg ccgccaccct cggcgcggtc gacaacatca tcggccccct ccctctctcc
      601 tcgagctcgg gcacgcgctc ccaacaactc cgcattaacc tctgggtgtg gatcgacgaa
      661 attcccgacg ctgcgcgcga cgtgaaagag gtcaaaaaaa tcaccgtcgt ttactcctac
      721 gatttcaacg acggcaaccg cgtgcggacc gttcgcgacc gcgaggtcat cctccgcacc
      781 cgcttcgacc aatgaaaacg cgtccgcttc gccgccgcag caacgccggc tacacgctca
      841 ccgaaatcat gatcacgacg accatcttcg ggatggtcgt cgccggcacg ctcacggttt
      901 tcatgatggg catgcgctcg atgtatcgcg acatcgagcg gctcagcacc gattccacgc
      961 tccgccggct cacgctccac gtcgccaagg agacaatcga ctccaccgag ttctacgtct
     1021 tccccaacta ccagagcctc gatggcagcg tgaacctcgt caccgatatc tcgccgctta
     1081 gtcccgccga acccgacgaa ggcgagacgc agctcgcgtc cggcgactgc ctcgtgctcg
     1141 tcacgcgcgt cacgatcgag tcgacgtcga acgtccgcca attccgcatc tactaccgcc
     1201 ccgtcacgtc ggccggcgcc caggcgccgc ttcgctatta cgagagcacc gactacggaa
     1261 gcgtcggcag cgaagattcg ctcaacgcgt tgctcaacgc cgttaacctt aaaaccacgc
     1321 ccgcctatcc cggatcgcgc gagctcgcca gcatcacccg cggcaaaccc attcccagca
     1381 gcacggacca tttccccgtg ttctccaccg aggccacggt gccgactgcc accaacgaga
     1441 gcgtttccct caacatcgag gtcatcaacg gtaagtccgc cgtgaacatg ctctccagct
     1501 ccagcttcaa ctacacgatc tcgccccggc gataattccc atgaaagctc cccgttccag
     1561 ccgccagcgc atccaaggtt acgcgcttct gtccgtcatc gtgttcgtcg ccatcatggc
     1621 cctgctcacc gcgagcatgc tgaaatacag cagcagcgaa cgccgcatga acgagcgcag
     1681 tcgcctcctc ctgcgcgccc gcaacatggc cgagaacgcc gcgctctacg cttccgagca
     1741 actcacgacg aagctctacc gcctccgctc cgccacgccg atggcgttca tgaccggcag
     1801 caatcagatc ttcctcccgc ccgacgaggt tctcactacg tctttctcgc agccctccga
     1861 cgtcgaagtg cgcgcgggtc tcacctacag cacggatctc gaatttatcg atccaacaga
     1921 ggccgaaaac gccaacaacc ccaacgccgg tctcagcgtc agcaccagca ccgttcccat
     1981 cgtcgccaaa gccacgatgc gccacccctc cctcggcgag gtcaccgccc acgtgg
//
LOCUS       DASPLT010000644         4645 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_8217, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000644 DASPLT010000000
VERSION     DASPLT010000644.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 4645)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4645)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4645
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8217"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..246
                     /locus_tag="VEA63_06695"
     CDS             <1..246
                     /locus_tag="VEA63_06695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD83720.1"
                     /translation="NRTTDLLLPSISHHADYLNIAGVSITAHTATFNPPAAFNKGSTT
                     SDVARAREDFRLALVAYDVVADVNRTVEMPDISVRTP"
     gene            657..3110
                     /locus_tag="VEA63_06700"
     CDS             657..3110
                     /locus_tag="VEA63_06700"
                     /inference="COORDINATES: protein motif:HMM:NF019113.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BNR-4 repeat-containing protein"
                     /protein_id="HYD83721.1"
                     /translation="MNRASLVSLLVGFGLTLARLTAGEPLTAAPTRLLPLADAAFSGS
                     SINVGANTRSSIVTYSTTQFAAFYDADGFMVLAKRALGADTWETRRTTHRANIADAHN
                     FISLAVDGAGFLHVAWDHHNNPLNYARSTAPLSLELAAKSPMTGERESSVTYPQFHLT
                     PNGDLLFLFRDGSSGNGSLVLHRYATADEKWSVVQSNLIDGEGQRSPYWDLNVDGRGT
                     LHLAWTWRDSPDVATNHDLAYARSTDGGATWQRTDGTPLALPLTATNCEYALRIPQNS
                     NLMNPPAIGTDRRGRPYISSYWSPAADAAPQFNIVQHTGSEWRIIPGPTPSQSFTLAG
                     TGTKRPPLSRAALLVDQTWDRLALHLIYRDDARGGRIVLATRAGYEPGEWRLRELNDT
                     DVGGWEPSFDPVSWQRLTQGQLLVQNVTQRDGDDRTTAMGSAKPISHLIWAPVTDRMR
                     ASSSHARTAAAHDATTTVAASSVNPAPRDPAAILALAQRAADWQWANFPPAEKRHPRG
                     WEVAPFYIGVLALDRVSPDHRNRERMLQQADALGWQPHQRIYHADDHCVIQAYLELYD
                     HYREPRMIGPSKERFDHILANPTTALFDWGTPNCGDHWSWCDALFMAPVAWLQLWKET
                     GDKRYLDFMNENWWLTTKRLYLPEVGFYLRDESYLDLREPNGKTIHWSRGNGWVFAGL
                     ARVLDLFPQDHADYPRYVKLYRDMAKAVLAAQQSDGLWRVGLLDPDTHTVRETSGTAF
                     FTYGLAWGLNRGLLDAADAKPAVQRGWQALASSVTADGKLENVQPIGHSPEGFDPHHT
                     DVFAVGAFLLAATEVSQLK"
     gene            3335..>4645
                     /locus_tag="VEA63_06705"
     CDS             3335..>4645
                     /locus_tag="VEA63_06705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046556 - alpha-L-arabinofuranosidase
                     activity [Evidence IEA];
                     GO_process: GO:0046373 - L-arabinose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-arabinofuranosidase C-terminal
                     domain-containing protein"
                     /protein_id="HYD83722.1"
                     /translation="MKTSLVPFAALLFVTSLSAAQTPASTSSSAASATATLTLHADQA
                     GAVINPNLYGHFAEHLGRCIYEGIWVGPDSPIPNTRGIRNDVVAALKQLNIPVLRWPG
                     GCFADEYHWKDGIGPREQRPAMINTHWGGVTENNHFGTHEFLDLVEQLGCEAYISGNL
                     GSGTVQEMMEWVEYMTSAADSPMANLRRANGRDQPWRVKYFGVGNESWGCGGNMTPEY
                     YANEYKRYNTFVKNYDRANPIYRIACGSNGEDYNWTDVLMRDAGRQMNGLSLHYYTLP
                     TGSWRGSKGSATDFAEDMYFATLRRTLRMDELVQKHGAIMDKYDPEKKVGMIIDEWGT
                     WYDVEPGTNPGFLYQQNTLRDALVAALNFHIFHKHADRVAMTNIAQTINVLQAVILTD
                     KEKMILTPTYHVFEMFKVHQGATVVPLELATPDYVHGDEKIPAVS"
BASE COUNT          871 a         1756 c         1232 g          786 t
ORIGIN      
        1 aaccgcacga ccgatctcct gctgccatcg atcagccatc acgccgatta tctgaacatc
       61 gcgggcgttt ccatcaccgc gcacaccgcc acgttcaacc cgccggccgc gttcaacaaa
      121 ggcagcacca cgagcgacgt cgctcgcgcc cgtgaagact tccgcctcgc gctcgtcgcg
      181 tatgacgtcg tcgccgacgt gaatcgcacc gtcgagatgc ccgacatctc ggtgcgcacg
      241 ccttgaagcg gtgcaatcat gtgtgctcgt tgtaggagcc tgcttgcagg cgattgcgcg
      301 acctaagccg gaaccatgcg tgaggatgta ggggcgccgc ttgtcggcgc cccgatttgc
      361 gagcgaagcg gcacggacgc aaggtccggc cctacactca ctccatggtg gcggcacccg
      421 cagaggaaga ttgagctttg gctgaatcgt tacggctgag gcggtcgccg cgccgtatcg
      481 cctgcaaaca agctcctaca tccgatcctt caatcggctc cgctatttct aaaccgccgc
      541 agccgtcgcc gcgaatccgt cagattctcc ttcgcctgcc gagcccggga cgctttgtca
      601 ccgggataac tggttaggga ttggcggcgt cccttcgagt cgttcacgtt gggctgatga
      661 atcgtgcctc gcttgtctca ctcctcgtcg gtttcggttt aaccctcgcc cgcctgacgg
      721 ctggcgaacc gctgacggcc gcgccgaccc gactccttcc gctcgccgac gccgccttct
      781 cgggcagctc catcaatgtc ggcgccaaca cccgctcgtc catcgtcacg tattcgacca
      841 cgcagttcgc tgcgttctac gacgccgacg gtttcatggt cctcgcgaaa cgcgccctcg
      901 gcgccgacac gtgggaaacc cgccgcacca cgcatcgcgc caacatcgcc gatgcgcata
      961 atttcatcag cctcgccgtc gacggcgccg gttttctcca cgtcgcgtgg gatcatcaca
     1021 acaaccccct caactacgcc cgctccaccg cgccgctctc gctcgagctt gccgccaaat
     1081 cgccgatgac cggcgaacgc gagtcgagcg tcacgtatcc acaattccac ctcacgccca
     1141 acggcgatct cctcttcctc ttccgcgacg gcagctccgg caacggctcg ctcgtgcttc
     1201 accgctacgc cacggccgac gaaaagtggt ccgtcgtgca atcgaacctc atcgatggcg
     1261 aaggtcagcg cagcccctat tgggatctca acgtcgacgg ccgcggcacg ctccacctcg
     1321 cgtggacctg gcgcgactcg cccgacgtcg ccaccaatca cgatctcgcc tacgcccgct
     1381 ccactgacgg cggcgcgacg tggcaacgca cggacggcac gccgctcgcg ctcccgttga
     1441 ccgccaccaa ttgcgaatac gcgctgcgca ttccccaaaa cagcaatctc atgaacccgc
     1501 ccgcgatcgg caccgatcgc cgcggccgcc cgtatatcag cagctactgg tcaccggccg
     1561 ccgacgccgc gccccagttc aacattgtgc aacacaccgg cagcgagtgg cgtatcattc
     1621 ccggccctac gccgtcgcag tcgttcaccc ttgccggcac cggcaccaag cgcccgccgc
     1681 tctcccgcgc cgcgctcctc gtcgaccaaa cctgggatcg cctcgccctg cacctcatct
     1741 atcgcgacga cgcccgcggc ggccgcatcg tgctcgcgac ccgggccggc tatgaacccg
     1801 gcgaatggag attgcgcgaa ctcaacgaca ccgacgtcgg cggctgggaa ccctccttcg
     1861 atcccgtttc ctggcaacgg ctcacgcaag gccagctcct cgtgcaaaac gtcacgcaac
     1921 gcgacggcga cgaccgcacg acggccatgg gctccgcgaa accgatttcc catctgatct
     1981 gggcgcccgt caccgatcgc atgcgcgcgt cttcctcgca cgcccgcacc gccgcggcgc
     2041 atgacgccac caccacggtc gccgcttcat ccgtcaaccc cgcgccacgc gaccccgcgg
     2101 cgatcctcgc gctcgcgcaa cgtgccgccg attggcaatg ggcgaatttc ccgcccgccg
     2161 agaaacgtca cccgcgcggc tgggaagtcg cgccgttcta catcggcgtc ctcgcgctcg
     2221 atcgcgtttc acccgatcac cgcaaccgcg agcgcatgct ccagcaagcc gacgcgctcg
     2281 gctggcagcc gcaccaacgc atctaccacg ccgacgacca ctgcgtcatc caagcctacc
     2341 tcgaactcta cgatcactac cgcgagccgc gcatgatcgg gccttcgaaa gaacgtttcg
     2401 accacatcct cgcaaatccc accaccgcgc tctttgactg gggcacgccg aattgcggcg
     2461 atcactggtc gtggtgcgat gcgctgttca tggctcccgt cgcgtggctc cagctttgga
     2521 aagaaaccgg cgacaagcgc tacctcgatt tcatgaacga gaactggtgg ctcaccacca
     2581 agcggcttta tcttcccgaa gtcggtttct acctgcgcga cgaatcctac ctcgatctcc
     2641 gcgagcccaa cggcaaaacc atccactggt cgcgcggtaa cggctgggtg ttcgccggcc
     2701 tcgcccgcgt gctcgatctc tttccgcagg atcacgccga ctatccgcgc tacgtgaaac
     2761 tctaccgcga catggccaag gccgttctcg ctgcgcaaca atccgacgga ctctggcgcg
     2821 tcggtctcct ggatccggat acacacaccg ttcgcgaaac cagcggcacg gcgttcttca
     2881 cctatggcct ggcgtggggt ctcaatcgcg gtctgctcga tgccgcggac gccaagcctg
     2941 cggtgcaacg cggctggcag gcactcgcga gcagcgtcac cgccgacggc aaactcgaaa
     3001 acgtccaacc catcggccat tcgccggaag gtttcgaccc gcaccacacc gacgtcttcg
     3061 ccgtcggtgc cttcctcctc gcggccaccg aagtcagcca gctcaagtag ggcagggctt
     3121 cagcccgccc tccgtagcgg caggcctccg tgcctgctgc tttgtaggtg aggcgccccc
     3181 gcgcgaaaag aagcagagca aaaccggcag tcccgacggc ctgccgctac gctccggttg
     3241 acggtcaaac gccacgccgt ttgtccgtca tcgccgcgcg cgctctcgta gcgaaacgcc
     3301 tgagcttttc gctccgccaa tccctcgccc catcatgaaa acgtctctcg tccctttcgc
     3361 cgcgctgctg ttcgtcacca gcctgtccgc cgcgcaaacg cccgcgtcca cctcctcatc
     3421 cgcagcatcc gccaccgcca cgctcacgct ccatgcggac caagccggcg ccgtcatcaa
     3481 ccccaatctc tacggccatt tcgccgagca tctcggccgc tgtatttacg aaggcatctg
     3541 ggtcggcccc gattcaccga tccccaacac ccgcggcatc cgcaatgacg tcgtcgccgc
     3601 gctcaagcag ctcaacattc cggtccttcg ctggccgggc ggctgcttcg ccgacgaata
     3661 ccattggaag gacggcatcg gcccgcgcga gcaacgcccc gccatgatca acacccactg
     3721 gggcggcgtc accgagaaca accacttcgg cacgcacgag tttctggacc tcgtcgaaca
     3781 gctcggctgc gaagcctaca tctccggcaa cctcggcagc ggcaccgtcc aggaaatgat
     3841 ggagtgggtc gaatacatga cctccgccgc cgactcgccg atggccaatc ttcgccgcgc
     3901 caacggccgc gatcagccgt ggcgcgtgaa atatttcggc gtcggcaacg agagctgggg
     3961 ctgcggcggc aacatgacgc ccgagtatta cgcaaacgaa tacaaacgct acaacacctt
     4021 cgtcaaaaac tacgaccgcg cgaaccccat ctaccgcatc gcctgcggct ccaacggcga
     4081 ggactacaac tggaccgacg tcctcatgcg cgacgccggc cgccagatga acgggctgtc
     4141 gctccactat tacacgctcc ccaccggcag ctggcgcggc tcgaaaggct ccgccaccga
     4201 cttcgccgaa gacatgtatt tcgccacgct ccgccgcacg ctgcgcatgg acgagctcgt
     4261 tcagaagcac ggcgcgatca tggacaaata cgacccggag aaaaaagtcg gcatgatcat
     4321 cgacgaatgg ggcacctggt atgacgtcga gcccggcacc aaccccggtt tcctttacca
     4381 gcaaaacacg ctgcgcgacg cgctcgtcgc cgcactcaac ttccacattt tccacaagca
     4441 cgccgatcgc gttgcgatga ccaacatcgc ccaaacgatc aacgtcctcc aggcggtgat
     4501 tctcaccgac aaggaaaaga tgattctcac gccgacctat cacgtcttcg agatgttcaa
     4561 ggttcatcaa ggcgcaaccg tcgttcccct cgagctcgcc acgcccgact acgtccacgg
     4621 cgatgaaaaa atccccgccg tcagc
//
LOCUS       DASPLT010000645         1112 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_67825, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000645 DASPLT010000000
VERSION     DASPLT010000645.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1112)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1112)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1112
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_67825"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..226
                     /locus_tag="VEA63_06710"
     CDS             <1..226
                     /locus_tag="VEA63_06710"
                     /inference="COORDINATES: protein motif:HMM:NF019229.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ASPIC/UnbV domain-containing protein"
                     /protein_id="HYD83723.1"
                     /translation="REAPGARVEVHTPRRVHVQTVAPAMSYMAQSTTTLTFGLGDDAR
                     VRKVVVQWPSGLRQEVRPEAVNRRIVVTEP"
     gene            362..859
                     /gene="rplI"
                     /locus_tag="VEA63_06715"
     CDS             362..859
                     /gene="rplI"
                     /locus_tag="VEA63_06715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HYD83724.1"
                     /translation="MAQSEILLVKPVENLGGEGDQVKVRAGYARNYLLPRGIAVPLTG
                     ANRKQVDALKKRRAEREMHELNGAQEVAKKLEKKSLAFAVKTGEGGKMFGAITASDIH
                     EKLVAEGLEIEKRRIHLHTPVKTLGQHTVKIKLHADVTVELNFDVVSENPIETAAPAV
                     EEKKK"
BASE COUNT          226 a          324 c          368 g          194 t
ORIGIN      
        1 gcgcgaggcg ccaggggcgc gcgtcgaagt gcacacgccg cggcgcgtgc acgtgcaaac
       61 cgtcgcgccg gcgatgagct acatggcgca gtcgacgacg acgttgacgt ttgggctcgg
      121 cgacgatgcg cgcgtgcgga aagtcgttgt gcaatggcct agcggtctgc ggcaggaggt
      181 gaggccggag gcagttaatc gtcggattgt cgtcacggag ccatgagggt gggcggggct
      241 gcgtgccagg cgattgaggt gttgacactc ggaggaaact ccggcgaatt ctgcaccctt
      301 tctctcgaat cccggtggcg aaaactgctg gcgaacgcat ccctttcaac caatccgtcc
      361 catggcccaa agcgaaatcc tcctcgtcaa acccgtcgaa aacctcggtg gcgaaggcga
      421 ccaagtcaaa gtccgcgccg gttatgcgcg caactatctc ctgccgcgcg ggatcgccgt
      481 gccgttgacg ggtgcgaatc gcaagcaggt cgatgcgctc aaaaagcgtc gcgccgagcg
      541 cgagatgcat gagctgaacg gcgcgcagga agtcgcgaag aagctggaga agaagagcct
      601 ggcgttcgcg gtgaagaccg gcgagggcgg caagatgttc ggcgccatca cggcgagcga
      661 catccacgag aagctcgtcg ccgaaggtct cgagatcgag aagcgccgca tccacctgca
      721 tacgccggtg aagacgctcg gccagcacac ggtgaagatc aagctgcacg ccgatgtgac
      781 ggtggagttg aacttcgacg tcgtgtcgga gaacccgatc gagacggcgg cgccggcggt
      841 cgaagagaag aagaaataat ctatttgtct gctttcccga cgaaggccgc gtccgttgcg
      901 acgcggcctt cgtgtttttg ggagcaaaat gttcaaaacc ctttattgcc ggcccgagtt
      961 cgcgtcctct cactgaaccg ctgcgcatgt ctgaagaagc caccattccg tttcgtcgtc
     1021 ccgagtccgc gcgctcggct gcaccgatcg ggcgaacgat gccgcacagc atcgaggccg
     1081 aggagtattt gctctcgtgc tgtttgctcg ac
//
LOCUS       DASPLT010000646         3345 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_172380, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000646 DASPLT010000000
VERSION     DASPLT010000646.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 3345)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3345)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3345
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_172380"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..295
                     /locus_tag="VEA63_06720"
     CDS             <1..295
                     /locus_tag="VEA63_06720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009513218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HYD83725.1"
                     /translation="AAGPLMAIYLLAMRLPKMEFVGTAAVFFMLLNLFKVPFMVNLGL
                     INAESFAINLWLAPAVFLGAWGGRKILVKIDQRLFENLALVLSAVAGVKLLWS"
     gene            complement(1033..2145)
                     /locus_tag="VEA63_06725"
     CDS             complement(1033..2145)
                     /locus_tag="VEA63_06725"
                     /inference="COORDINATES: protein motif:HMM:NF012870.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HYD83726.1"
                     /translation="MTRKLTFAAACREAGISRQCGYKWWRRFRRKGAAGLKERSHRSR
                     RAESLQRRWCARVLALRRRHRTWGAPKIRGYLKRRHARAALPGVSTISRWLAEAGLVR
                     PRVRWTRPGPRVPAAARAPARQPNDVWTVDFKGSFRTHDARRVYALTVRDATTHYVLA
                     VRHAPKPDDVTVRTIFIRLFRRYGLPRALQMDNGTPFGAVGPLGLSRLSVWWLRLGIE
                     LRFSRPACPQDNPAHEQMHGVLKAETTRPPSANPQAQQRRFDRWRTIYNHVRPHAALA
                     NASPADHYRPSPRRYPATLPDWSYPPNTATLCPGTNGRAWWQQRQRLIGRAFAGERLG
                     LQITPTCTQVRLGPFLIGTLHAEDMAGLRPARWTRP"
     gene            complement(2382..3296)
                     /gene="bla"
                     /locus_tag="VEA63_06730"
     CDS             complement(2382..3296)
                     /gene="bla"
                     /locus_tag="VEA63_06730"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: protein motif:HMM:NF033105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="subclass B3 metallo-beta-lactamase"
                     /protein_id="HYD83727.1"
                     /translation="MKAFFSSTRMIIGFAAAGGAAFAATPPVDRAAALAMSAAWNAPV
                     PPRHLVGNIYYVGAMGVSSFLITTPEGHVLLDTGLPATAPQIVSGVEQLGFKVSDIKL
                     ILSSHAHFDHTGSHATLQRLTDAKVVASAEDARVLESGGAADFIPFPKDLAAYEPVKV
                     DRVVADEEGVKLGGVMLTAHVTPGHTRGATTWTMEVEEEGRTYRVVFFSSASINPGTR
                     LTGPGAAYPEIADDLARSFSAFRAMRCDLFFAPHGGQFAMGEKFTRLDAGEKGEVVFA
                     DPAGWRALIDGQEKAFRELLVAERAEAK"
BASE COUNT          608 a         1096 c         1035 g          606 t
ORIGIN      
        1 tgcggcgggg ccgttgatgg cgatctatct gctggcgatg cggctgccga agatggagtt
       61 cgtcgggacg gcggcggtgt ttttcatgct gctgaatttg ttcaaggtgc cgttcatggt
      121 gaacctcggg ttgatcaacg cggagagttt cgcgatcaat ctgtggctcg cgcccgcggt
      181 gtttctcggg gcgtggggag ggcggaaaat tttggtgaag atcgaccagc ggctgttcga
      241 aaatctggcg ttggtgttga gcgcggtggc gggggtgaag ctgttgtgga gctgagccgc
      301 atcgattcag ccagcgctca atctttcgct gcgggtgccc gccaccatgg agtgagtgta
      361 ggaccggacc ttgcgtccgt gccgcttcgg tcgcaatcgg gcgccggcaa gcggcgcccc
      421 tacctcctca ctccatggtt ccggctaagt cggattcatc gagttgagaa accacgaatg
      481 gacacgaatg aacacgaatg aacacgaatt cagaggaggt ggaggaaagc tttggaccga
      541 ttgtgtgcat caccgtttgg tgttccgatc cgattctgca tccgaactcg attcattcgt
      601 gtcggttcgt gtccattcgt ggttgaacgg aatttttccg gctaagggag gatgaaaggt
      661 gagagtgaaa gtagggtcct ccgcgcctgc gcgcttgatt gccgattgcg cactcaccct
      721 gacgtagcgc ggtcaacctg accgcgcccg cgcgtctcca agcacgggca agtggcggtt
      781 gctcgctgag tggtgacact ttatgctcac ggagtggtga cactcgatga ggttgttgag
      841 gggttgttgg ggagcgaagc tctccagcgg tccggcggct tgaaagattc caaaggaatc
      901 ggggtggaga ggggtgaggg gagaggcggg gctgccccgc ctttcccctc tgcggaaccg
      961 acgtcgtggt tcaacgatgg tgaagaaaaa gagttgcttg ggatgagcgc atgcttggga
     1021 caccgcctgt agctatgggc gtgtccatcg tgccggtcgc agcccagcca tgtcttcggc
     1081 atggagtgtg ccgatcagga aaggaccgag gcgcacctgc gtgcaagtcg gggtaatttg
     1141 gaggccgagc cgctcgccgg caaacgcgcg accaatcagg cgttggcgct gttgccacca
     1201 cgcccggccg ttggtgccgg ggcagagggt ggcggtattc ggtgggtagg accaatccgg
     1261 gagcgtagcc ggatagcgtc gcggactggg gcggtagtgg tcggcggggg atgcgttggc
     1321 caaggcggcg tgaggccgga cgtggttgta gatcgtgcgc cagcggtcga agcggcgctg
     1381 ttgcgcttgg gggttggccg acggcggccg cgtggtttcc gctttgagca cgccgtgcat
     1441 ctgttcgtgc gccggattat cctgcgggca ggcgggccgg gagaacctta gttcaatccc
     1501 taaccgcaac caccaaacgc tcagtcgcga caaccccagc ggcccgaccg cgccgaaagg
     1561 cgtgccattg tccatttgga gcgcgcgagg cagcccgtag cggcgaaaca agcggatgaa
     1621 gatcgtgcgc accgtgacgt cgtcgggctt gggtgcatga cgcacggcga gcacataatg
     1681 agtcgtcgcg tcccgcacgg tcagggcgta gacgcgccgt gcgtcgtggg tgcgaaacga
     1741 gcccttgaaa tcgaccgtcc acacatcgtt gggctggcgc gccggcgccc gtgcagccgc
     1801 cggcacgcgg ggtccaggcc gagtccaccg aacccggggc cgcaccagcc ccgcctccgc
     1861 gagccatcgg ctgatcgtgc tcacccctgg caacgccgcc cgcgcgtggc gccgcttcag
     1921 atacccgcga atcttcggcg caccccaggt gcgatgtcgc cgtcgcaacg ccagcacccg
     1981 cgcgcaccag cgccgctgca acgactccgc gcgacgcgac cggtgcgacc gctcctttaa
     2041 tccagccgcg cccttgcggc gaaaacgccg ccaccacttg taaccacact gccggctgat
     2101 cccggcctca cggcaagcgg ccgcgaacgt tagctttctg gtcaaaatcg tcgcgatgaa
     2161 ttgccaacgg gcaccgcgtt tctggggttc tttccaaggc atatcgcctc agcaaagtgt
     2221 caccactcag tgagcataaa gtgtaaccga tcagtgaaca accgccggtt gaccgtgcta
     2281 ccttccgagc attcgtggct cgccgcgtga gcggcgggat ttgtgacgca cgtcccgccc
     2341 cccttgcgca tcaatcccca cgctcacgca cagcgccacc gtcactttgc ctcggcccgt
     2401 tccgccacca gcaattccct aaacgccttc tcctgcccat cgatcaacgc acgccagccc
     2461 gccggatcgg caaaaacgac ctcgcccttc tcgcccgcat cgagccgcgt gaacttctcc
     2521 cccatcgcga actgcccgcc atgcggcgcg aaaaacaaat cgcaccgcat cgcgcgaaac
     2581 gcgctaaaac tccgcgccaa atcgtccgca atttctggat acgcggcgcc cggccccgtc
     2641 agccgtgtcc ccggattgat cgacgcgctg ctaaagaaca cgacgcgata agttcgccct
     2701 tcctcctcaa cctccatcgt ccacgtcgtc gccccgcgcg tgtgccccgg cgtcacgtgc
     2761 gccgtcaaca tcaccccgcc gagcttcacc ccctcctcat ccgccacgac gcgatccacc
     2821 ttcaccggct catacgccgc aagatccttc ggaaacggaa tgaaatccgc cgctccaccc
     2881 gactccagca cgcgcgcatc ctccgcgctc gcgacgacct tcgcgtccgt caaccgctgc
     2941 aacgtcgcat ggctccccgt gtgatcgaaa tgcgcatggc tgctcaggat gagtttgatg
     3001 tccgacacct tgaacccgag ctgttccact ccgctcacga tctgcggcgc cgtggccggc
     3061 agccccgtat ccagcagcac atgcccctcc ggcgtcgtga tcaaaaacga gctcactccc
     3121 atcgcgccca cataataaat attgccgacg agatgccgcg gcggcaccgg cgcattccac
     3181 gccgccgaca tcgccagcgc cgccgcgcga tccaccggcg gtgtcgccgc gaacgccgcc
     3241 cctccggccg ccgcgaaacc aatgatcatc cgcgtgctcg aaaagaaagc cttcataagc
     3301 ctgtgaacga acagtccgca ccgcactcgt caacgatgcg gcacc
//
LOCUS       DASPLT010000647         1079 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_90310, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000647 DASPLT010000000
VERSION     DASPLT010000647.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1079)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1079)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1079
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_90310"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..717
                     /locus_tag="VEA63_06735"
     CDS             <1..717
                     /locus_tag="VEA63_06735"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HYD83728.1"
                     /translation="DECPPWPCERDECARAVARSHRWAKQCKEIASESGFLAGGHHVF
                     AIVQGSTFDDLRRQAAESLSALDFPGYAVGGVSVGEPEPEMLKQVGATTPFLPADKPR
                     YTMGLGTPPQLLKMIALGVDMFDCVLPTRVARNGLVFTPDGPMNLRNEKYRADPRPIV
                     EGLDNYTCRTFSRAYLRHVTLAGEIVSCTLLTLHNLHFYLNLMALAREHLEAGDFGAW
                     HLAWIERYEAGAAARASGAA"
BASE COUNT          201 a          285 c          400 g          193 t
ORIGIN      
        1 gacgagtgtc cgccgtggcc gtgcgagcgc gatgagtgcg cgcgggcggt ggcgcgaagc
       61 caccggtggg cgaagcagtg caaggagatc gcgagcgaaa gcggcttcct cgcgggcggg
      121 catcatgtgt tcgcgatcgt gcaaggatcg acgtttgacg atctgcggcg tcaggcggcg
      181 gaatcgctga gcgcgttgga ttttcccgga tatgcagtgg gcggcgtcag cgtgggcgag
      241 cccgagccgg aaatgttgaa acaggtcggc gcgacgacgc cgtttctgcc ggcggacaaa
      301 ccacgttaca ccatgggcct gggcacgccg ccgcagctgc tgaagatgat cgcgctgggg
      361 gtggatatgt tcgattgcgt gctgccgacg cgagtcgcgc gcaacggcct ggtgttcacg
      421 ccggacgggc cgatgaattt acgaaacgag aagtatcgcg ccgatccgcg gccgatcgtg
      481 gaagggctgg acaattacac gtgccggacg ttttcgcgcg cgtatctgcg gcacgtgacg
      541 ctcgcggggg agatcgtcag ttgcacgctg ctgacgctgc acaacttgca cttctacctc
      601 aatctgatgg cgctagcgcg agaacacctc gaggcgggcg acttcggcgc gtggcatctc
      661 gcgtggatcg agcgttacga agcgggcgcg gcggcgcggg cgagtggtgc ggcgtagccg
      721 gtaggctctg tttttaaccg ccgcttgtta tgcgatgagg cgcgccggcc ggcggggcga
      781 gacgcgctcg tcgggcgcgg tcagttttgt ccgtcgtggg acacgttgga ctcggtcgcg
      841 acgagcacga caaggagaag cagaacaata caagcggcta acatggtgtg tgttggtgtg
      901 tagggcgacg aagtgtagtg ggcttcgctg cgttacggaa tacgcatcct tgcgcaggtc
      961 agggcggtcg gatgggtagc gagagaacgc gagggctgag accatcgcga gcccgaacat
     1021 tgttccatca atgatagtag ccgtttcacc aaggctagaa accggcgcaa taaaaggag
//
LOCUS       DASPLT010000648         1339 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_90315, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000648 DASPLT010000000
VERSION     DASPLT010000648.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1339)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1339)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1339
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_90315"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            83..283
                     /locus_tag="VEA63_06740"
     CDS             83..283
                     /locus_tag="VEA63_06740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="JAB domain-containing protein"
                     /protein_id="HYD83729.1"
                     /translation="MQRVGRRAQHPSGDPSPSAADVNVTRKLREAAKILEIELLDHVV
                     VGDSKGDPQGRGVYSFREAGLL"
     gene            360..677
                     /locus_tag="VEA63_06745"
     CDS             360..677
                     /locus_tag="VEA63_06745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD83730.1"
                     /translation="MKACLPHQPTDTEREAAFANFFATVRATARESDAAVLAAKPALE
                     RIAQAVVGRDNGQALRVRSILLSLYTGGAMLADVSDLMTLDWSLRRDLCAVLLTASSA
                     TII"
     gene            674..1000
                     /locus_tag="VEA63_06750"
     CDS             674..1000
                     /locus_tag="VEA63_06750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD83731.1"
                     /translation="MRTAFQDASDARWFLGAAPDPIERLREALDFVKPGPLTTTTTST
                     LSERGVAEFLASLFGGVPVELGSALQSLDQIRAALVMDVSSDFISRRFGDEAVALVRE
                     HFSLDA"
BASE COUNT          249 a          394 c          405 g          291 t
ORIGIN      
        1 gtcggcacct tatcacgctg ggaacggttg attcgacact ggtttcctgt cgcgaggttt
       61 tccgcggcgc gatcattgct ggatgcagcg cgttggtcgt cgcgcacaac acccgagcgg
      121 cgatccgtcg ccgagcgcgg ccgatgtgaa cgtcacgcga aagctccgcg aagccgcgaa
      181 gatcctcgag atcgagttgc tcgaccacgt ggtggtcggt gattcgaaag gcgacccgca
      241 aggccgcggg gtctattcgt ttcgcgaagc ggggctcctc taggaggagc gtccgcagcc
      301 gccccgtaag gggcggcgtc atctccggac ctacgaagca tcccccgtag acttccgcga
      361 tgaaagcctg tctccctcat caaccgacgg acaccgaacg tgaggccgcg ttcgcgaatt
      421 tctttgccac ggtgcgcgcg actgcgcgcg agagtgatgc tgcggtgctc gccgcgaagc
      481 ccgctttgga gcgaatcgcc caagctgtcg ttgggcgaga caatgggcaa gcgttgcgcg
      541 tgcgttcaat cctgctgtcg ctctacacgg gtggggctat gctggcggac gtctccgact
      601 tgatgacgct cgattggtcg ttgcgccgag acctctgcgc ggtgctcctc acggcgagtt
      661 cggctacgat tatatgagga cggctttcca ggacgcaagt gatgcacgat ggtttctcgg
      721 cgccgccccc gatccgatcg aacgactacg cgaagccctc gattttgtga aacccggccc
      781 gcttacaaca acaacaacca gtacgctaag cgaacggggc gtcgcggaat tcttggcgag
      841 tctttttggc ggggtgccgg tagagctggg aagcgcgtta cagagtctcg accagattcg
      901 cgcagcgctt gtcatggacg tgtcgtccga tttcatttcg cgccgatttg gcgacgaggc
      961 agtcgccctc gtgcgtgagc atttttccct cgacgcgtaa atcggatagc acgctcgtta
     1021 ggggcattcc tcttgcccgg cggtggcgga aggtaagaat gttcgagcca tgtcctccaa
     1081 cgcgattgcc ttacgtttca gtgattccgg tagggggcgg ttcggcgatc atggccacgt
     1141 aactctttgg gtaacactta aacctttttg tcatcgcaat aatgcgataa agcatttgac
     1201 ctgtcatgcc tgtttgccac gttgttttct accaggaaca ggacggaaac gcccccgttg
     1261 tcgattggct caaagaacta cgaaacaccg atgcgaaagc ttttaccaaa tgtcgcgccg
     1321 ccctcgctcg tctcgccca
//
LOCUS       DASPLT010000649         1525 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_192832, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000649 DASPLT010000000
VERSION     DASPLT010000649.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1525)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1525)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1525
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_192832"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1525)
                     /locus_tag="VEA63_06755"
     CDS             complement(<1..>1525)
                     /locus_tag="VEA63_06755"
                     /inference="COORDINATES: protein motif:HMM:NF039178.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MBG domain-containing protein"
                     /protein_id="HYD83732.1"
                     /translation="LNFPRSADGANSYGFGLVIQGGDSAITTVGLGTVNSWGAMSVEG
                     GRLAIDELTIGWQQTANRGGQARVLGGELVVDQLVMSRKNGSNANNIAELHLLGGITT
                     LERLTLGYDADVNAGSATINLNGGALFVGAGGIVRHGVAPFATAINLIDGTLGAKDDW
                     ASGVAFNLASDNTVAIHTADAANVPHNLTLVGVIGGAGGFTKTGDGTLMLAGANTFTG
                     SVAIDAGTLLVTGSLAAGGDVTINTGGALAGSGTIEKTIVLNGGTLAPDGSTAAAAVT
                     WNPGSLLAFRVGANGVSDQLALSGALTRGGSGTFPIALSPAIGFAAGNTYTLATFGST
                     DFAASDFTAVGLPAGHLAIFSITDTSLQVTIKAAASLTLGNLTQAYDGTPKSASATTT
                     PAGLAITLNYDGNTTPPTLPGRYAVTATIDDPAYVGSASGTLVITVTALVRHAPTLNG
                     DIEGSVQVLLPENVTFNSGALISGDLLVPGTPAIQLNGSPTYGSTLDQSGAATPASHR
                     "
BASE COUNT          239 a          432 c          585 g          269 t
ORIGIN      
        1 tcgatgactc gcgggagtcg cggcaccgga ctggtcgagc gtgctgccgt aagtcgggct
       61 accattgagt tgaatggcgg gcgtgccggg gacgagaaga tcgccggaga tcaacgcacc
      121 gctgttgaag gtgacgtttt ccggcaggag cacttgaacc gacccttcga tgtcgccgtt
      181 gagcgtcggc gcgtggcgaa ccagtgcggt gaccgtgatc acgagcgtgc cgctcgcgct
      241 gccaacgtag gccggatcgt cgatcgtggc ggtgacggcg taacgacccg gaagcgtcgg
      301 gggcgtcgta ttgccgtcgt agttgagagt gatggcgaga ccggcgggcg tggtggtcgc
      361 gctggcagat tttggcgtgc cgtcgtaagc ttgcgtgagg ttgccgagag tgagacttgc
      421 ggccgccttg atcgtaacct ggaggctcgt gtcggtgata ctgaaaatcg ccaggtggcc
      481 ggcggggagg ccgacggccg tgaagtcgct cgcggcaaaa tcggtggagc cgaacgtcgc
      541 gagcgtgtaa gtgttgccag cggcgaagcc gatggccggc gagagtgcga tcgggaacgt
      601 gccgctgccg cctcgcgtga gcgcgccgct gagggcgagt tgatcggaga ctccgttcgc
      661 gccgacgcgg aacgcgagga gactgccggg attccaagtg acggcggcgg ctgcggtgct
      721 gccgtcggga gccagcgtgc cgccgttgag gacaatggtt ttttcgatcg tgccggagcc
      781 ggcgagcgcg ccgccggtgt tgatcgttac gtcgcccccg gcggcgagac tgccagtgac
      841 gaggagggtg cccgcgtcga ttgcaacgga accggtgaac gtgtttgcgc ccgcgagcat
      901 gagtgtgccg tcgccggttt tcgtgaagcc gccggcgcca ccgatgacac cgacaagcgt
      961 gagattgtgc ggaacgttcg cggcgtcggc ggtgtggatc gcgacggtgt tgtcgctggc
     1021 gaggttgaac gcgacaccgg acgcccagtc gtctttcgcg ccgagcgtgc cgtcgatgag
     1081 attgatcgcg gtggcgaagg gcgcgacacc gtggcggacg atgccgccag cgccgacgaa
     1141 gagcgcgccg ccattgaggt tgatggtggc ggagccggcg ttcacgtcgg cgtcgtagcc
     1201 gagggtgagg cgttcaagcg tagtgatgcc gccgagaagg tggagttcgg cgatgttgtt
     1261 ggcgttcgag ccgttcttgc gcgacatgac gagttggtcg acgacgagct cgccgccgag
     1321 cacgcgcgcc tggccaccgc gattcgcggt ttgctgccag ccgatcgtca gttcgtcgat
     1381 cgcgaggcgt ccgccctcca cggacattgc gccccacgag ttgacggtgc ccagcccgac
     1441 agtcgtgatc gcgctgtcgc caccttggat gacgaggccg aaaccgtagg agttggcgcc
     1501 gtccgcgctg cgcgggaagt tgagc
//
LOCUS       DASPLT010000650         2190 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_253671, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000650 DASPLT010000000
VERSION     DASPLT010000650.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 2190)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2190)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2190
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_253671"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1696)
                     /locus_tag="VEA63_06760"
     CDS             complement(<1..1696)
                     /locus_tag="VEA63_06760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007359261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreAB"
                     /protein_id="HYD83733.1"
                     /translation="MNSEAVSALIAKNPPLKAAKAKLEAMEPGAYVIHRSWGFGQIKS
                     YDDASQRLLIDFKDKKAHPMDPAFCVNTMEVLPPKHLLVRKETESARIAELIADDPAQ
                     LIAEALESYPNHATTQIDLEITLAQVIGEEKFKKWWAVAKKAIAKDPRIQVPEKKTEC
                     FILRETPVSAEDEILEQFNSTRSARRRIALAEELIAAVGKKDVKTDLTAILASVADGV
                     KSSNQLDPAERLYGAFVRDELARHINVDPATFDPQQSALIANTRDLPAIAEKIPVHFQ
                     GQFLELVNATHPIESRDLLFTLLKTSQGKFTTECINFLVEQGHADELAAALKRWQTEQ
                     NLRAPVLLWIVKNRHSKKFAKLLNDLITPRLLGAIFFAIDYEALQASSARRIPLADIL
                     SEDTDLIADLLSTADPETARDLANTLMLNQGFEELTKKSLLARFIKIFPKIQSLVAAD
                     AESKEEQLLVSKPSFERKREEYETIVSKKIPENSKAIATAREHGDLKENSEYKMAKQD
                     QQVLMAQKTQLERDLGRARITDFKEATTEQVSVGSIVEVKNTATGAVTRYTLLGAWD"
     gene            1776..>2190
                     /locus_tag="VEA63_06765"
     CDS             1776..>2190
                     /locus_tag="VEA63_06765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HYD83734.1"
                     /translation="MSVATEPSKPDAWAAAARLIARWLERRERVDELLETLGASLSGA
                     ERARCQHLVFGVVRHFGRIEAVLGRLIAHPPRFSTRAVLFIAGFELIDASGKAMADGL
                     PPKIVHHAVEQTKRLASPAEAKLVNAVVRKLAGVLR"
BASE COUNT          360 a          548 c          800 g          482 t
ORIGIN      
        1 cgtcccaagc cccgagcagc gtgtaacgcg tgacggcgcc agtggcggtg ttcttgacct
       61 ccacgatcga gccgacgctg acctgctcgg tggtggcttc cttgaagtcg gtgatccggg
      121 cgcggccgag gtcgcgctcg agctgggttt tctgggccat gaggacctgc tggtcctgct
      181 tggccatctt gtattcggag ttttccttga ggtcgccgtg ttcgcgagcg gtggcgatgg
      241 ccttggagtt ctccggaatt ttcttggaga cgatcgtttc gtattcctcg cgcttgcgct
      301 cgaacgacgg cttcgaaacg aggagttgct cttccttgct ttcggcgtcg gcggcgacga
      361 gcgactggat cttcgggaag attttgatga agcgggcgag gagcgacttc ttcgtgagtt
      421 cttcgaagcc ttggttcagc atcaacgtgt tcgcgagatc gcgggccgtc tccggatcgg
      481 cggtcgagag aagatcggcg atgagatcgg tgtcttcgct gaggatatcc gcgagcggga
      541 tacggcgggc gctggaagct tggagcgcct cgtaatcgat cgcgaagaaa atggcgccga
      601 ggaggcgggg tgtgatgaga tcgttgagga gcttcgcgaa tttcttcgag tggcggttct
      661 tgacgatcca aaggaggacg ggcgcgcgca gattttgctc ggtctgccag cgcttgagag
      721 cggcggcgag ttcgtcggca tggccttgtt cgacgaggaa gttaatgcac tcggtggtga
      781 acttgccctg cgacgtcttg agcaacgtga agagcaggtc gcgggattcg atcgggtggg
      841 tggcgttcac cagttccagg aactggcctt ggaaatgaac ggggatcttc tcggcgatgg
      901 cggggagatc gcgggtgttg gcgatcagcg cggactgctg cggatcgaac gtggccgggt
      961 cgacgttgat atggcgggcg agctcatcgc ggacaaaggc accgtagagg cgttcggccg
     1021 gatcgagctg gttactggac ttcacgccgt cggcgacgct cgcgagaatg gcggtgaggt
     1081 cggtcttaac gtctttcttg ccgaccgcgg cgatgagctc ctcggcgagg gcgatgcggc
     1141 ggcgggcgga gcgagtggag ttgaactgtt cgagaatctc gtcctcggcg gaaaccgggg
     1201 tttcacgcag gataaagcat tcggttttct tttccgggac ctggatgcgc gggtccttgg
     1261 cgatggcttt cttcgcgacg gcccaccact tcttgaactt ttcctcgccg atgacctggg
     1321 cgagcgtgat ctcgagatcg atctgggtgg tggcgtgatt cgggtaagat tcgagcgctt
     1381 cggcgatgag ttgagcgggg tcgtcggcga tgagttcggc gatgcgggcc gactcggttt
     1441 ctttgcggac gaggaggtgc ttcggcggga gaacctccat ggtgttgacg cagaaagccg
     1501 ggtccatcgg atgggccttc ttgtccttga aatcgatcag gaggcgttgc gaggcatcgt
     1561 catagctctt gatttgaccg aacccccagc tgcggtgaat cacgtaagcg cccggctcca
     1621 tggcttcgag tttggctttg gccgctttga gcggcgggtt cttggcgatg agcgcggaaa
     1681 cggcttcgga attcataatt gcgaaagtga tggcctgaac gggagagttg agcggagaat
     1741 gatcagcctt gtcaaacaga gtgtcgaata cggccatgag cgttgcaact gaaccgagca
     1801 aacccgatgc gtgggcagcc gcggcgcgtt tgatcgcgcg ctggttggag cggcgcgaac
     1861 gggtggacga actgctggag acgctgggag cgtcgttgag tggtgccgag cgggcgcggt
     1921 gtcagcacct ggtgtttggg gtggtgcggc attttggtcg gatcgaggcg gtgctcgggc
     1981 ggttgatcgc gcatccgccg cgtttctcga cgcgcgccgt gctgtttatc gcggggttcg
     2041 agttgatcga cgcgtccggc aaagcgatgg cggatgggct gccgccaaag attgtgcatc
     2101 atgccgtcga gcagacaaag cgcctggcga gcccggccga ggcgaagctg gtgaacgcgg
     2161 tcgtgcgcaa actcgccggc gtgcttcgcg
//
LOCUS       DASPLT010000651         2402 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_8313, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000651 DASPLT010000000
VERSION     DASPLT010000651.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 2402)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2402)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2402
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8313"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2402)
                     /locus_tag="VEA63_06770"
     CDS             complement(<1..>2402)
                     /locus_tag="VEA63_06770"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HYD83735.1"
                     /translation="VSNVGVESALNQLPQFVPSQTQFSAGDIQSFAYNTPGIATLNLR
                     GLGSNRNLVLVDGRRAQPANALLVVNVNTIPSAAIESVEVISGGASATYGADAMGGVT
                     NFKLKKNFEGFNLSVQGSDTEQGGGGETRISGLMGASFGSGNVMLGLEGSERQEIRNI
                     DRDFYLDGFRDPGTPSGVSSLRFASYKPAGASTAPSAAAVAAIFGTAPFAPGADSFFV
                     NPDKATMFLNTPASGAVGYTGPQNDEFKIIKGVAGAGALNQNNIEAAFSTPMERYSIF
                     SRANYDFSDSLSAYMQANFTNSTVVTNAAYSPALSQWNATIPRDGRALPAQLNALLDS
                     RANPAGSWPLEMGFIDFSGTRRNVNRDKEFQVLAGITGDIFSTGWTFDAYASHGETAL
                     VAELENYPMLQPFRAIIQAPNFGKNFISTSGPPLFFATKCTSGLPVFENFEISQDCID
                     SIYAQMKHLTSIDQDIIEINTQGEVMDLPAGTLQAALGVSTRRNSFAFRPDALLDTHS
                     IEYPIGLFPTSPTTGETEVKEAYAEALVPIVQDLPGIQDFNLELGARYSDYDTSGGIW
                     TYKGLIDWSVTDELRIRGGYQLANRAPNTAELYLGSSTSVVGFAGGDPCLTNTLNEWG
                     NLPSNPNQAQVVALCNLINEKSGYPNTPYKQNPNAIVGPFAFNFPLELAISSGNPNLR
                     NEEAETWTLGFVFNSPFEGGLFERATLAVDWYEIDIVDAIQIGDSWSVYAKCFNQDGS
                     NPTYSYVEACTLIARDQDGYRATVTTPYQNLGGIQTSGIDVQLNWGFDAIGGSLNLSS
                     VV"
BASE COUNT          420 a          728 c          778 g          476 t
ORIGIN      
        1 cacgacgctg ctcaggttca ggctgccccc gatcgcgtcg aagccccagt tcaattgcac
       61 gtcgatgccg gaggtctgga tgccgccgag gttctggtac ggcgtcgtta ccgttgcgcg
      121 gtagccgtcc tggtcgcgcg caatcagcgt gcaagcttcg acgtaggaat acgtcgggtt
      181 gctgccgtcc tggttgaaac acttcgcata caccgaccag ctgtcgccga tctggatcgc
      241 atcgacgata tcgatctcgt accagtccac tgccagtgtg gcgcgttcga acagcccgcc
      301 ttcgaacggc gagttgaaca cgaagccgag cgtccacgtt tccgcttcct cgttgcgcag
      361 gttcggattg ccgctcgaga tcgcaagttc cagcgggaag ttgaacgcga acggaccgac
      421 gatcgcattc gggttctgtt tgtacggcgt attcggatag ccgctcttct cgttgatcag
      481 gttgcacagc gcaacgacct gcgcctggtt cgggttgctc ggcaggttgc cccactcgtt
      541 cagcgtgttc gtcaggcatg gatcgccgcc ggcaaagcca accaccgacg tcgacgaacc
      601 gagatacagt tccgccgtgt tcggcgcgcg gttggcaagc tggtagccgc cgcgaatgcg
      661 caactcgtcg gtaacgctcc agtcgatcag gcccttgtag gtccagatgc cgcccgacgt
      721 gtcgtaatcg gaatagcgcg caccgagctc gagattgaag tcctggatgc ccggcaggtc
      781 ctgcacgatt ggcacgagcg cctcggcata ggcttccttc acttcggttt cgccagtggt
      841 gggcgacgtg gggaacaggc caatcggata ctcgatgctg tgcgtatcga gcagcgcatc
      901 gggacggaac gcgaaactgt tccggcgcgt cgacacgccg agcgcggcct gcagcgtgcc
      961 ggccggcaga tccatcactt cgccctgcgt gttgatctcg atgatgtcct ggtcgatgga
     1021 cgtcaggtgt ttcatctgcg cgtagatcga atcgatgcag tcctgcgaga tctcgaagtt
     1081 ctcgaacacc ggcaaaccgc tcgtgcactt cgtggcgaag aacagcggcg ggcccgacgt
     1141 cgagatgaag ttcttgccga aattcggcgc ctggatgatc gcgcggaacg gctgcagcat
     1201 cggatagttc tcgagttcgg cgacgagcgc ggtttcgccg tgcgaggcat acgcatcgaa
     1261 ggtccagccg gtgctgaaga tgtcgccggt gatgccggcg agcacctgga attccttgtc
     1321 gcggttcacg ttgcggcgcg tgccggagaa atcgatgaag cccatttcca gtggccacga
     1381 tccggcgggg ttcgcgcgtg aatcgagcag cgcattcagc tgcgcgggca acgcgcgacc
     1441 atcgcgcgga atcgtagcgt tccactgcga caatgccggc gagtacgctg cgttcgtcac
     1501 gaccgtcgag ttcgtgaagt tcgcctgcat gtacgccgac aggctgtcgg agaaatcgta
     1561 attcgcgcgc gagaagatcg agtagcgttc catcggtgtc gagaaagccg cttcgatgtt
     1621 gttctggttc agcgcgccgg cgccggcgac gcccttgatg atcttgaact cgtcgttctg
     1681 cggcccggta taaccgaccg cgccggatgc cggggtgttc aggaacatcg tggccttgtc
     1741 agggttgacg aagaacgagt cggcgccagg agcgaacggc gccgtgccga aaattgccgc
     1801 gaccgccgca gcgctcggcg ccgtggatgc accggccggt ttatacgacg cgaagcgcag
     1861 gctcgacacg cccgacggcg tgccgggatc gcgaaaacca tcgaggtaaa agtcgcgatc
     1921 gatgttgcga atctcctggc gctcggagcc ttcgaggccg agcatcacgt tgccactgcc
     1981 gaagctcgcg cccatcaggc cgctgatgcg ggtttcgcca ccgccaccct gttcggtgtc
     2041 gctgccctgc accgacaggt tgaagccttc gaagttcttt ttcagtttga agttggtgac
     2101 gccacccatg gcgtcggcgc cgtacgtggc cgacgcgccg ccactgatca cttcgacgct
     2161 ttcgatcgcc gccgacggaa tcgtgttgac gttgacgacg agtagcgcgt tcgccggctg
     2221 cgcgcggcgg ccgtcgacga gtacgaggtt gcggttcgag ccgaggccgc gcaggttcag
     2281 cgtagcgatg ccgggcgtgt tgtaggcgaa cgactggatg tcaccggcgc tgaactgcgt
     2341 ttgcgacggc acgaactgcg gcagctggtt cagcgcgctt tcgacgccga cgttgcttac
     2401 ct
//
LOCUS       DASPLT010000652         1517 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_8325, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000652 DASPLT010000000
VERSION     DASPLT010000652.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1517)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1517)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1517
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8325"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            225..>1517
                     /locus_tag="VEA63_06775"
     CDS             225..>1517
                     /locus_tag="VEA63_06775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009510118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HYD83736.1"
                     /translation="MTRSLAAVAMGREAPDLVITGGRVLSTYTERIHDDREIWVKGGR
                     IAAVKAAGTFKAAKGAANKTKLYDARGGILAPGLVDPHIHIESAMMTACAYAEGALLN
                     GTTTIFCDSHEIGNVCDAAGIEWMLEDARQAPLSIFLTVPSTVPVTTPKFETAGGDLT
                     ARKIGKLFDKWPEAVALGEKMDFVQVAMGDVRSHAILGEAIKRGRPVCGHIYGREFVA
                     AGAASGITDTHEAIDREIANDFLEAGVWVFLRGGPPTTPWHSLPQAIKAVTELGANPK
                     RVCVCTDDRDADDLFLFGMDWVTREAVKAGLPPVTAWSMGSLHGATRYAKDGDIGGLG
                     HSRRADVVLLNDALEVQNTWFGGELVVEGRKVTPLLDKALSRRYRYPRAAYQTVKLPK
                     KVKLTPALPTKPVTANVIRTALPGIILLHEKVSLAPAAG"
BASE COUNT          283 a          363 c          600 g          271 t
ORIGIN      
        1 gtggcgcggg ttttgagttc gtcgaaacgg ctggagaagg aatcggcgaa cgatgaagaa
       61 cacgcggcga ggcacgcgat cgcggaaaga aaccggaggc tcatgaggtg aaacggaagc
      121 aggcgtcgcg ccgggtcgcg acgcaaaaac ggctttcgct ggcgtgcggt ttgcttttgg
      181 gtgggcgcat gctcacgcgt ttctccgttg ccccgcttca cacgatgact cgctcgctgg
      241 ccgcggtggc catggggcgc gaagctccgg atctggtgat cacgggcggg cgcgtgttgt
      301 cgacttacac ggagcggatt catgacgacc gcgagatttg ggtgaaaggc gggcggatcg
      361 cggcggtgaa ggcggcgggg acgttcaagg cggcgaaagg cgcggcgaac aagacgaagc
      421 tttacgatgc gcgcggaggg attctggcgc cgggattggt cgatccgcac attcatatcg
      481 agagcgcgat gatgacggcg tgcgcgtatg cggaaggggc gttgttgaat ggaacgacga
      541 cgattttttg cgacagccac gagatcggca acgtgtgcga tgcggcggga atcgagtgga
      601 tgttggagga tgcgcggcag gcgccgttgt cgatttttct gacagtgccg agcacggtgc
      661 cggtgacgac gccgaagttt gagacggcgg gcggcgattt gacggcgagg aagattggga
      721 agctgttcga caagtggcca gaggcggtgg cgctcggcga gaagatggac ttcgtccagg
      781 tcgcgatggg cgacgtccgg agtcacgcga tccttggtga agcgatcaag cgggggcggc
      841 cggtgtgcgg acacatctac gggcgggagt tcgtggcggc aggggcggcg agtggaatca
      901 cggatacgca cgaggcgatc gaccgggaga tcgccaacga ttttctggag gcgggtgtct
      961 gggtttttct gcgcggcggt ccgccgacga cgccgtggca ttcgctgccg caggcgatca
     1021 aagcggtgac ggagctcggc gcgaatccga agcgcgtatg cgtgtgcacg gacgatcggg
     1081 acgcggacga tttgtttttg ttcggaatgg attgggtgac gcgcgaggcg gtgaaggccg
     1141 ggctgccgcc ggtgacggcg tggagcatgg gttcactgca cggtgcgacg cggtatgcaa
     1201 aagatggaga catcggcggg ctcggtcact cgcggcgcgc ggatgttgtg ttgttgaacg
     1261 atgcactgga ggtgcagaac acgtggttcg gaggcgagct ggtggtcgaa ggacggaaag
     1321 tgacgccgct tttggataaa gcgctgagcc ggcgttatcg ttatccgcgc gcggcgtatc
     1381 aaacggtgaa gctgccgaag aaggtgaagc tcacgccggc gctgccgacg aagccggtga
     1441 cggcgaacgt gattcggacg gcgttgccgg ggatcatttt gttgcacgaa aaagtgtcgc
     1501 tcgcgccggc ggcgggc
//
LOCUS       DASPLT010000653         1741 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_232869, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000653 DASPLT010000000
VERSION     DASPLT010000653.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1741)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1741)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1741
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_232869"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1202
                     /gene="argA"
                     /locus_tag="VEA63_06780"
     CDS             <1..1202
                     /gene="argA"
                     /locus_tag="VEA63_06780"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007360364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004042 - acetyl-CoA:L-glutamate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HYD83737.1"
                     /translation="IVADENFSNLLVDLAVLRSLAIKVVIVHGIGHQLRELAVARNIA
                     ITNVDGSGVTDAATLDLAIRASSRVSHALIEGLTQNGLKCAVTNAVRALPLGIIKGVD
                     HQFTSKVERIDKDFLTELIDRQVIPVVSPIAFGPDGRSLRVNSDLLAAELAEALHATK
                     IIYLTPQPGLEIDGVVRREISVDAVRKLLAEHPEQISEATRSKAAHAVKAIETGTPRV
                     HILDGRIFDGLLNEIFSNEGVGSLVYGNDYAQIRKARKSDVRLIYNLTRAAVRCEELL
                     HRTQQAIEKNIDHFFVFEIDENIIACVTLYFYPDKSQLAEVGSLYVMPFYHNRGIGKK
                     MVEYAVLVAKERGAKKVIALSTQSFGFFQNVVGFEEADKSILPEARLKSYEESGRNAK
                     VLVKQLA"
     gene            complement(1409..>1741)
                     /locus_tag="VEA63_06785"
     CDS             complement(1409..>1741)
                     /locus_tag="VEA63_06785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD83738.1"
                     /translation="RAGGTGSAISFVTADAAAHFRLVEKRHGLSLPRERLRGFEPTDV
                     AAPVRDPHGGVKGKRKSKKDKAREAAGRAANPVEEKTAEASGMETAKETTELFPWRPR
                     VPRAGRGI"
BASE COUNT          328 a          648 c          453 g          312 t
ORIGIN      
        1 cgatcgttgc cgacgagaat ttcagcaatc tcctcgtcga cctcgccgtt ctccgctcgc
       61 tcgcgatcaa ggtcgtgatc gtccacggca tcggccacca gctccgcgaa ctcgccgtcg
      121 cccgcaacat cgcgatcacc aatgtcgatg gttccggcgt caccgacgcc gccacgctcg
      181 atctcgcgat tcgcgcctcc tcgcgcgttt cgcacgcgct tatcgaagga ctcacgcaaa
      241 acggcttgaa atgcgccgtc acaaacgccg tgcgcgcgct cccgctcggc atcatcaagg
      301 gcgtcgatca ccagttcacc agcaaggtcg aacgcatcga caaggatttc ctcaccgagc
      361 tgatcgatcg ccaggtcatc cccgtcgtgt ccccgatcgc cttcggcccc gacggacgct
      421 cgctccgcgt gaactccgac ctcctcgccg ccgaactcgc cgaggcgctt cacgccacga
      481 aaatcattta tctcacgccg cagcccggcc tcgaaatcga cggcgtcgtt cgccgcgaaa
      541 tttccgtcga cgccgtgcgc aagctcctcg ccgagcatcc cgagcaaatc tccgaagcca
      601 cccgcagcaa ggccgctcac gccgtcaaag cgatcgagac cggcacgcca cgcgtgcaca
      661 tcctcgatgg ccgcatcttc gacggcctgc tcaacgaaat tttttccaac gaaggcgtcg
      721 gctcgctcgt ctacggcaac gactacgcgc aaatccggaa agcccgcaaa agcgacgtcc
      781 gcctcatcta caatctcacc cgcgccgccg tccgttgcga ggagctgctt catcgcacgc
      841 agcaggccat cgagaaaaac atcgatcact tcttcgtctt cgaaatcgac gagaacatca
      901 tcgcctgcgt cacgctctat ttttatcccg acaagtcgca gctcgccgag gtcggctcgc
      961 tctacgtcat gccgttctac cacaaccgcg gcatcggcaa gaaaatggtc gaatacgccg
     1021 tgttggttgc gaaggaacgc ggcgcgaaaa aagtgatcgc gctctcgacg cagagcttcg
     1081 gctttttcca gaacgtcgtc ggcttcgagg aagccgacaa atcgatcctc cccgaagccc
     1141 gcctgaagag ctacgaggaa agcggccgca acgcgaaagt cctggtcaag caactcgcgt
     1201 gaccaagcag ctcgcgtgaa ggtggagccc gacgtccccg tcggactcca cctttccgtc
     1261 tgcaattctc gcggccagac ggcttctcct gcatgagtcc aacgcagtga ttgcctcaac
     1321 cgaacgcgct tcgccgatta ggcgaagcac gcaggccctc gcccgcccct cccacctccg
     1381 aaaattagcg caaattagcg gttaaacctc agattccgcg ccccgctcgc ggcacccgcg
     1441 gccgccacgg aaacagctcc gtcgtctctt tcgctgtctc catcccgctc gcttccgccg
     1501 ttttttcctc taccggattc gccgcccgcc ccgccgcttc gcgcgccttg tccttcttgc
     1561 tcttccgctt ccccttcacg cctccgtgcg gatcgcggac gggcgccgcc acgtcggtcg
     1621 gttcgaagcc gcggaggcgc tcgcgcggga gagacaaccc gtgacgcttc tcgacgagcc
     1681 ggaagtgcgc ggccgcatcc gcggtgacga agctgatcgc ggagcccgtc ccgccggcgc
     1741 g
//
LOCUS       DASPLT010000654         1003 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_90388, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000654 DASPLT010000000
VERSION     DASPLT010000654.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1003)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1003)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1003
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_90388"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(157..>1003)
                     /locus_tag="VEA63_06790"
     CDS             complement(157..>1003)
                     /locus_tag="VEA63_06790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD83739.1"
                     /translation="LAVFVDDTVVDQLERLHGVLASLEPAPRIARWLVFHETKDATPP
                     CALAAARQVFSTSRFAAPIGGGATDNFTELNYHPEVSASADFTVHACNPQVHAFDEAS
                     IVETLAMQGVTVANARQLSAGKPVVVSPVTFIRRWRLNDVGAPSGIPAGLMPFQRDPR
                     HASAFNAAWTLGSLAALARAGADSLTFFETHGDNGLLSSAGTPLPVHGVFAALHGTTG
                     AAVLPTSSSHPLHADALALHSGARPIVHLANFRGENQRARVTGPWGTHVLELAPYATQ
                     TFQLP"
BASE COUNT          190 a          285 c          363 g          165 t
ORIGIN      
        1 caccggattc cgaatacgcg tcgggcagca ccacgttgac cggaatatcc ttgtgcatcg
       61 atgcactggc gatggtgcgc gtttcgacct tggcgccgaa ggctgatgac gccgcaacga
      121 atagcgcgag taatccagcg aaagagagtc gagttgtcat ggaagttgaa aggtctgcgt
      181 cgcgtagggt gctagttcga gcacgtgggt gccccaaggg ccggtcacgc gcgcgcgttg
      241 attctcgccg cgaaaattcg cgaggtggac gattggccgc gcacccgaat gaagcgcgag
      301 cgcatccgcg tggagcgggt gcgaagaact cgtcggaagc accgcagcgc ccgtggttcc
      361 gtgcaacgcg gcgaagacgc catgcaccgg gagcggcgtg ccggcggaag agagcaggcc
      421 gttgtcgccg tgcgtttcga aaaacgtgag gctgtcggcg ccggcgcgcg cgagcgccgc
      481 gaggctgccg agtgtccacg cggcgttaaa tgctgacgcg tgtcgtggat cgcgttgaaa
      541 cggcatgagg ccagcgggaa tcccgctcgg cgcgccgacg tcgttcaggc gccacctgcg
      601 gatgaacgtc accggcgaga ccacgacggg tttgccggcg gagagctggc gggcattggc
      661 gaccgtgacg ccttgcatcg cgagcgtttc gacgatcgag gcttcgtcga aggcgtgcac
      721 ctgcgggttg caggcgtgca ctgtgaaatc cgccgaggct gaaacctccg gatggtagtt
      781 gagctcagta aaattgtcgg tcgcgccgcc gccgattgga gcggcgaagc gtgaggttga
      841 aaaaacctgt cgcgccgcgg cgagcgcgca gggaggtgtc gcatctttcg tttcgtgaaa
      901 caccagccag cgagcgattc gcggagccgg ctcgagcgac gcgagaacgc cgtgcaatcg
      961 ttcgagttgg tcgacaaccg tgtcgtcgac gaagacggcg agc
//
LOCUS       DASPLT010000655         1069 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_294919, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000655 DASPLT010000000
VERSION     DASPLT010000655.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1069)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1069)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1069
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_294919"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..994
                     /locus_tag="VEA63_06795"
     CDS             <1..994
                     /locus_tag="VEA63_06795"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HYD83740.1"
                     /translation="SDSTLWNPAIFVQDSIKLGEKFALLVGARGDAFFAKARDPLGAA
                     AGDNWADEHDDTALSWNASVIHYLTPRASLYVTHQRAFAVHGNVTGGGVMLKDDGNGR
                     GIIDPDDFKNLSRLTEVGAKFSLLDNKLYAGVALFDQRREEVELGGDVKNLKFRGGEL
                     EFVYQPDTHFNATFNVALTDGRFDNSSASQAGGTSLYNLYALGAGPGGRGNGLGFQWD
                     KLPRGDYRIPGLSRWIVNTSFSYRLRNGFGGGLGGSWQSEQPGNLLNEYHIPAQVFLN
                     VFLFYRQPTWEVNVDVLNVLDRRNWIHNGDNFSNNVLVFQDLPLRVEGYVKFKF"
BASE COUNT          203 a          304 c          352 g          210 t
ORIGIN      
        1 gtcggactcg acgctttgga atccggccat cttcgtgcag gattcgatca agctcggtga
       61 aaaatttgcg ctgttggtgg gtgcgcgcgg cgatgcgttt ttcgcgaagg cgcgcgatcc
      121 gctcggcgct gcggcggggg ataactgggc ggacgaacat gacgacaccg cgttgtcgtg
      181 gaatgcgagt gtcatccact acctgacacc gcgcgcgtcg ctctacgtga cgcatcagcg
      241 cgcgttcgcg gtgcacggca atgtgacggg cggcggcgtg atgttgaagg acgacggcaa
      301 cggtcgcggc atcatcgatc ccgacgattt caaaaacctc agccggctca cggaagtggg
      361 cgcgaagttt tcgctgttgg acaacaagct ctatgccggc gtggcgctct tcgaccagcg
      421 gcgcgaggag gtcgagctcg gaggcgacgt gaagaacctg aagtttcgcg gcggtgagct
      481 ggagttcgtt taccagcccg acacgcattt caacgcgacc ttcaacgtcg cgctcaccga
      541 cgggcgcttc gacaactcgt cggcgtcgca agccggcggc acgtcgctct acaatctcta
      601 cgcgctggga gcggggccgg ggggacgggg caacggactc ggtttccaat gggacaaact
      661 gccgcgcggc gattatcgaa ttccgggact gtcgcgttgg atcgtgaaca ccagtttctc
      721 ctatcgtctc agaaacggct tcggaggcgg cctgggaggc agctggcaaa gcgagcagcc
      781 gggcaacttg ctcaacgagt atcacattcc ggcgcaggtc ttcctgaacg tattcttgtt
      841 ctaccgccag ccgacgtggg aggtgaacgt cgatgtcctc aacgtgctcg atcggcgcaa
      901 ctggatccac aacggagaca acttctcgaa caacgtgctg gtctttcagg atctgccgct
      961 gcgcgtcgaa ggctacgtta agttcaagtt ctagttcctg tagtcaggat gtgggggcgc
     1021 cgcttgtcgg cgcaccgatt tgcgagcgaa gcggcacgga cgcaaggtc
//
LOCUS       DASPLT010000656         1474 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_8411, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000656 DASPLT010000000
VERSION     DASPLT010000656.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1474)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1474)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1474
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8411"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..314)
                     /locus_tag="VEA63_06800"
     CDS             complement(<1..314)
                     /locus_tag="VEA63_06800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD83741.1"
                     /translation="MLTLRGSPSLSPFRLQKLLADLTAAAVPVRALSAEFVHVAELVD
                     AKNPLTDVEHAVLEKLLTYGPSRATQRVTGLTQVVAPRPGTISPWSSKATDIAHICGL
                     TK"
     gene            641..>1474
                     /locus_tag="VEA63_06805"
     CDS             641..>1474
                     /locus_tag="VEA63_06805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HYD83742.1"
                     /translation="MSEHIGHECGIAVVRLLKPLSYFQEKYGTPLWGFEKLFLLMEKQ
                     HNRGQDGAGVACVKLDMPAGEPYMFRERSIQPNPLDRVFGEVLGRYRELVSAGTVHPE
                     FPDTVKKHFDFGAELYLGHLRYGTSGGYGVSACHPYFRRSSWPTRNLALCGNFNMTNT
                     RELNDSLIEIGQHPIFASDTQAVLEKIGFYLDEEHEDVYRYLRTRGLEGVEMSRKISE
                     DLDLARVFTRASQNWDGGYAIAGLVGNGDAFVARDPSGIRPAFWFQNDEVVAFASERA
                     PL"
BASE COUNT          285 a          353 c          545 g          291 t
ORIGIN      
        1 attttggtga ggccgcagat gtgcgcgatg tcggtggctt tggaggacca gggcgagatg
       61 gtgccggggc ggggtgcgac gacttgagtg aggccggtga cgcgttgcgt ggcgcggctg
      121 gggccgtagg tgagaagttt ctcgaggacg gcgtgttcga catcggtgag cggatttttc
      181 gcatcgacga gttcggcgac gtgaacgaac tcggcgctga gcgcgcggac cggcacggcg
      241 gcggcggtga ggtcggcgag aagcttttga aggcgaaagg gcgagaggga aggcgagccg
      301 cgaagcgtca gcatgatgaa aagcgaatgt ttcgggagtt cgcgggcgtg gtcaagggga
      361 gcgaggaggg aagctccaag ctccaagatc caagctccag ggaaacatca catcccaacc
      421 gcaaaattag ggcgtgagtt tgaatgtgga ggtttgtcgg ggcgtaaagc ccctcccacc
      481 gtgttggatt gcgtgcggtt tggagaaagc cgttgaccaa attggacacg tcgccgaaat
      541 cagacctttt aacgcatgat ggagacgacc aacctggctg ccccggttat cttgggcagg
      601 gagtctgaat cgccggccga ccgccggtgt cactcctagt atgagcgaac atatcggcca
      661 cgagtgcggg atcgcggtgg tgcggctgtt gaagccgctc tcatattttc aggagaaata
      721 cgggacgccg ttgtggggtt tcgagaagct tttcctgttg atggagaagc agcacaatcg
      781 cggacaggac ggcgcgggcg tggcctgcgt gaagctggat atgccggcgg gagagccgta
      841 tatgtttcgt gagcggagca ttcagccaaa tccgctcgac cgggtgttcg gcgaggtgtt
      901 gggacggtat cgcgagctgg tgagcgcggg cacggtgcat ccggagtttc cggacacggt
      961 gaagaagcat ttcgatttcg gcgcggagct ttatctcggg catttgcgtt acgggacttc
     1021 gggcggctac ggcgtgagcg cgtgccatcc gtattttcgg agaagttcgt ggccgacgcg
     1081 gaatctcgcg ttgtgcggca acttcaacat gaccaacacg cgggagttga acgacagcct
     1141 gatcgagatc gggcagcatc cgatcttcgc gtcggacacg caggcggtgc tggagaaaat
     1201 cggcttctac ctcgatgaag agcacgagga tgtttaccgc tacctgcgca cgcgcgggct
     1261 cgaaggcgtg gagatgtcgc ggaagatcag cgaggatcta gacttggcgc gggtgttcac
     1321 gcgcgcttcg cagaattggg acggcggtta cgcgatcgcc gggctggtgg gcaacggtga
     1381 tgcattcgtg gcgcgcgatc catcggggat ccggccggcg ttttggtttc agaacgacga
     1441 agtggtggcg tttgcgtcgg aacgcgcgcc gttg
//
LOCUS       DASPLT010000657         1649 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_31671, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000657 DASPLT010000000
VERSION     DASPLT010000657.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1649)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1649)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1649
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_31671"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..744)
                     /locus_tag="VEA63_06810"
     CDS             complement(<1..744)
                     /locus_tag="VEA63_06810"
                     /inference="COORDINATES: protein motif:HMM:TIGR00056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HYD83743.1"
                     /translation="MKQLNAILEGIGSTVLLLVRSLSYLGTLPRQSARFIEQCFMIGY
                     TTLPIVAILSFFIGSVLALQAGYAMQNFGAKQFLGVLVGLSMARELGPVMVAILLAGR
                     VGSAIAAELASMKVYQEIDALETMNIPPARMLVLPRLAAVLCMMPVLTIIADLVGWFG
                     GAVVAKFVSMISIDPPAYFAAMRQQMEFEDVLNGMIKAEVFGFVVVLVCCNIGLNTRG
                     GPREIGAAVTRAVVTSLILILVLDYFVTKA"
     gene            complement(746..>1649)
                     /locus_tag="VEA63_06815"
     CDS             complement(746..>1649)
                     /locus_tag="VEA63_06815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD83744.1"
                     /translation="FAFWPLYGRLEKPGVFERRFYLWPFAWNNTIQTRHADGAPVADA
                     APRREFGVLPFYTAETAPGFVNKSYLWPFFGYTDRTEPTRYHETRYFWPFIVRGEGDD
                     RDVNRFGPFYTHSEVKGVEKTWIAWPIYRERNWTDAGLVHEQRQVLYFLYRSTKQRSA
                     TNPAAASAEKAHLWPLISRWDNGAGRRQFQFPSPLEVFFPDNERIRVSWSPLFALYRF
                     DQQAPDRRRHELLWGLFTKRTTPELRETHLGPLFTVKEREGEKRIAIGNGLLSWRRSA
                     PGARWRFFWFDFPRKDRTLRASHR"
BASE COUNT          345 a          469 c          558 g          277 t
ORIGIN      
        1 ggctttcgtg acgaagtaat cgagcacgag gatgaggatg agcgaggtga ccaccgcgcg
       61 ggttacggcg gcgccgatct cgcggggacc gccgcgtgta ttcagaccga tgttacagca
      121 aacgagcacg acgacgaagc cgaagacctc ggccttgatc atgccgttga gcacgtcttc
      181 gaactccatc tgctgccgca tcgcggcgaa atacgcgggc ggatcgatcg agatcatgct
      241 gacgaatttc gcgaccacgg cgccgccgaa ccatccgacg aggtcggcga tgatcgtgag
      301 cacgggcatc atgcagagca cggcggcgag acgaggcagc accagcatgc gcgcgggggg
      361 aatgttcatc gtctcgagcg catcgatctc ttgataaact ttcatcgagg cgagctccgc
      421 ggcgatggcg gacccaactc ggccggcgag aagaatggcg accatcacgg gaccgagttc
      481 gcgcgccatc gagaggccga cgagcacgcc gaggaactgt ttcgcaccga aattctgcat
      541 cgcgtagccg gcctgcaaag cgagcacgct gccgatgaag aagctcagga tcgcgacgat
      601 gggcagtgtc gtataaccga tcatgaaaca ctgctcgatg aaacgagcgc tctgacgcgg
      661 gagcgtgccc aaatatgaaa ggctgcgcac gagaagcagc acggtgctgc cgatgccctc
      721 gaggatggcg ttgagttgtt tcatgtcagc ggtgcgacgc gcgaagcgtg cgatctttgc
      781 gggggaaatc gaaccagaag aaccgccagc gcgcgccggg agcgctgcgg cgccacgaga
      841 gcaggccgtt gccgatcgcg atgcgtttct cgccttcgcg ctcttttacg gtgaagagcg
      901 ggccaagatg cgtctcgcgc agctccggtg tcgtgcgttt cgtgaacaag ccccagagca
      961 actcgtggcg gcgacgatcg ggagcttgct ggtcgaaacg gtagagggcg aacagcggcg
     1021 accacgacac gcggatgcgt tcgttatcgg ggaagaacac ttcgagcgga ctgggaaact
     1081 ggaattggcg gcggccggcg ccgttgtccc aacggctgat gagcggccag aggtgcgcct
     1141 tttccgcgga cgcggcggcg ggattcgtag cgctgcgctg tttcgtggag cggtagagaa
     1201 aatagagcac ctgacgctgt tcgtgaacga gtcccgcatc agtccagttg cgctcgcgat
     1261 agatcggcca ggcgatccac gtcttttcga cgcctttgac ttccgaatgc gtgtagaaag
     1321 ggccgaaacg attcacgtcc cggtcgtcgc cttcgccgcg aacgataaac ggccagaagt
     1381 agcgcgtctc gtgataccgc gtgggttccg tgcgatcggt gtagccgaag aagggccaca
     1441 gatagctttt gttgacgaag cccggcgcgg tttccgcggt gtagaaggga agcacgccaa
     1501 actcacgacg cggcgcggcg tcggcgacgg gtgcgccgtc ggcgtggcgc gtctggatcg
     1561 tgttgttcca ggcgaacggc catagataga aacgccgctc gaacacgccg ggcttttcga
     1621 gccggccata caatggccag aacgcaaag
//
LOCUS       DASPLT010000658         1354 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_90437, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000658 DASPLT010000000
VERSION     DASPLT010000658.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1354)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1354)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1354
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_90437"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..450
                     /gene="modA"
                     /locus_tag="VEA63_06820"
     CDS             <1..450
                     /gene="modA"
                     /locus_tag="VEA63_06820"
                     /inference="COORDINATES: protein motif:HMM:NF024921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0030973 - molybdate ion binding [Evidence
                     IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HYD83745.1"
                     /translation="VLWTTRDDLALPTIADAVRNRSTKRIAIANPDTAPYGRAALQVL
                     EKLGVRAEAEPKIVIGENITQTAQFVATGNAEIGFVALSLLLSPKLKERGRWLEVPAN
                     LHSPLEQGAVLTRRGAENPAAKTYLTFLRSATAREILERHGYRAPMR"
     gene            631..1323
                     /gene="modB"
                     /locus_tag="VEA63_06825"
     CDS             631..1323
                     /gene="modB"
                     /locus_tag="VEA63_06825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HYD83746.1"
                     /translation="MLDSASTFLGLSATLWQSLGLTLKLAAITTLVLGLVGLPLAHWL
                     NTTRSRFAAIIETISTLPIVLPPTVIGFYLLVSFSPNHPPGSWWVALTGSPLSFSFTG
                     LVIASVFYSLPFAVQPFQAALRGVPPELIDAGRALGAKPFRVWWRVHVPLAWRGIVAG
                     LTLGFAHTLGEFGVVLMIGGSIPGVTRVASIALYDEVQKLNYTQAHAFAAILLVLSFV
                     LLLIVTLLQRRR"
BASE COUNT          212 a          497 c          377 g          268 t
ORIGIN      
        1 gtgctctgga ccacacgcga cgacctcgcc ctcccaacga tcgccgacgc cgttcgcaat
       61 cggtcgacga agcgaatcgc catcgcaaat cccgacaccg ctccctacgg ccgggcggcc
      121 ctgcaagttc tcgaaaagct cggcgtgcga gccgaagccg agccaaagat cgtcatcggc
      181 gagaacatca cgcaaaccgc gcagtttgtc gccaccggca acgccgagat cggcttcgtg
      241 gcactctcac tcttgctatc gccgaaattg aaagagcgcg gccgctggct tgaggtgccg
      301 gcgaatctcc actccccgct ggaacaaggc gccgtgctca cgcgccgcgg tgcggaaaat
      361 cccgccgcaa aaacttatct cacgttcctg cgaagcgcga ctgctcgcga aatcctcgaa
      421 cgccatggct atcgcgcgcc catgaggtag ggcccgacct ccgggcgggc cgattccgcc
      481 cggctgctcg caggacgtgc gctttcaagc caatcagcgc gagcaagctc gccgcctacc
      541 actcacaccc cgggcttcac cgctccacaa tcttcgcccc tccaacgatc gcgttgaggt
      601 gttcgcttcg gcccgccaac gtctctcgcg ttgctcgact ccgcctccac gtttctcggt
      661 ctctccgcga ccctctggca atcgctcggc ctcacgctga agttggccgc gatcaccacg
      721 ctcgtgctcg ggcttgtcgg acttccgctc gcgcactggc tcaacaccac acgttcgcgg
      781 tttgcggcga tcatcgaaac gatctcaacc ctgccgatcg tccttccgcc gaccgtcatc
      841 ggtttctacc tgctcgtgag tttctcgccc aatcacccgc cgggtagctg gtgggtcgcg
      901 ctcacgggtt cgccgctttc gttttcgttc accggactcg tgatcgcctc ggtcttctac
      961 tcgctgccat tcgccgtgca gccgtttcaa gccgcactgc gcggcgtgcc acccgaattg
     1021 atcgacgccg gacgcgctct gggcgcgaag ccctttcgcg tctggtggcg cgtgcacgtg
     1081 ccgctggcgt ggcgtggcat cgttgcgggc ctcacgctcg gcttcgcgca caccctcggt
     1141 gagttcggtg tcgtgctcat gatcggcggg tcgattcccg gcgtgacccg cgtcgccagc
     1201 atcgcgctct acgatgaggt gcaaaaactc aactacacgc aggctcatgc cttcgccgcc
     1261 atactgctcg tgctctcgtt cgtgctgctg ctgattgtga cacttctgca acgccgccgc
     1321 taggtttaga cgcgccaccg cgcgggcctt gacg
//
LOCUS       DASPLT010000659         1559 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_232997, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000659 DASPLT010000000
VERSION     DASPLT010000659.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1559)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1559)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1559
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_232997"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1559)
                     /locus_tag="VEA63_06830"
     CDS             complement(<1..>1559)
                     /locus_tag="VEA63_06830"
                     /inference="COORDINATES: protein motif:HMM:NF037085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBG domain-containing protein"
                     /protein_id="HYD83747.1"
                     /translation="GRAIVWANQSTRFLGTLKANAGAAGGNGGFVEVSGKTFLDFNPR
                     SVVDLSAANGKTGTLLLDPAALHIGATNPGTAVSFLDVSILEDQLANGNVTLETTIND
                     GDGTVTFAAPVAWESANTLRVESFDDIYLEADLTAPHGAIEFYPGPSGTFQPQNQDGG
                     MRFLSPRVEQAADTTLEAGRLTYGTNDVPLPEEWDAEGGAGSAFFDGNLNVGTIEIDL
                     DHGGTGIIAGGINNTISSIRTIGTGALEFFYVENHHGPLNLRLNSTNATAGAITAISA
                     GNLTLEAGSEFNFNNTESRNAVLASRDGNVVNQAGAAAFGEKTRAVFYSNTNVPSNKG
                     GLAGIDIYNHAFPADGSEDLDGFATSNLFFFAGDSGLPLLTYTANNLSRLYGAPNPTF
                     TATVTGLLNDDQLADVVTGAPSFSTAATQSSGVNTYAITVAQGTLTSTNYGFDFVPGT
                     LTITPAPLTITANDTDRRLNRNNPTFSASFDGFVNGDTSAVVSGLQFSTTATLVSPAG
                     NYAITPYGATAA"
BASE COUNT          273 a          452 c          537 g          297 t
ORIGIN      
        1 gccgcggtcg caccgtaagg cgtgatggcg tagttgccgg ccggagacac gagcgtcgca
       61 gtcgtggaga actgcaggcc ggaaaccacc gccgacgtgt caccgtttac gaagccatcg
      121 aagctcgcgc tgaaggtggg attgttgcga ttgaggcggc ggtcggtgtc attggccgtg
      181 atcgtgagcg gcgccggcgt gatcgtcagc gtgccaggca cgaagtcgaa gccgtagttc
      241 gtcgaggtca gcgtgccttg cgcaacggtg atcgcatagg tgttcacccc ggagctctgc
      301 gtcgcggcgg ttgagaagct gggcgcgccg gtgacgacgt cagcgagttg atcgtcgttg
      361 agcaggcccg tgaccgtcgc cgtgaaggtt ggattcggcg caccatatag gcgactcagg
      421 ttgttcgccg tataagtgag cagcggcagg ccggaatcac ccgcgaagaa aaagagattg
      481 ctcgtggcga atccgtcgag gtcttccgag ccgtccgcag gaaaggcatg gttgtaaatg
      541 tcgatgcctg cgagaccacc cttgttcgac gggacgttcg tgttgctgta gaaaacggcg
      601 cgcgtcttct cgccgaaagc ggcggcgccc gcctggttga ccacgttgcc gtcgcgcgag
      661 gcgaggacgg cgttgcgcga ttcggtgttg ttgaaattga actcgctgcc ggcttcgagc
      721 gtgagattgc ccgcgctgat cgccgtgatg gccccggcgg tggcgttcgt cgaattgagc
      781 ctcaggttga gcggcccgtg atgattctcc acgtagaaaa attcaagggc acccgtgccg
      841 atggtgcgga tcgagctgat cgtgttgttg atgccgccgg cgatgatgcc ggtgccgccg
      901 tggtcaaggt cgatctcgat ggtgccgacg ttgagattgc cgtcgaagaa cgcgctgccg
      961 gcgccgccct ccgcatccca ttcctcgggc agcggcacat cgttggtgcc ataggtgagc
     1021 cgaccggcct ccagcgtcgt gtcggcagcc tgctcgacgc gcggagaaag aaaccgcatg
     1081 cctccgtcct ggttttgcgg ctgaaaggtg ccggaggggc cggggtaaaa ctcaatggcg
     1141 ccatgagggg cggtgaggtc cgcttccaaa tagatatcgt cgaacgattc cacgcgcagc
     1201 gtgttcgcgc tctcccacgc gaccggcgcg gcgaaggtca ccgtgccgtc gccgtcgttg
     1261 atcgtcgtct cgagcgtcac gttgccgttc gcgagctggt cttccagaat gctgacatcg
     1321 aggaaagaca ccgcggtgcc gggattggtg gcgccgatgt gcagcgccgc gggatcgagc
     1381 agcagcgtgc cggtttttcc attggcggcc gaaagatcga cgactgagcg cggattgaaa
     1441 tcaagaaacg tcttgccgga aacttccacg aaaccgccat tgccgcccgc ggcgcccgca
     1501 ttcgccttca gcgtgccgag gaaacgcgtg ctctggttcg cccacacgat cgcgcgacc
//
LOCUS       DASPLT010000660         1714 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_213082, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000660 DASPLT010000000
VERSION     DASPLT010000660.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1714)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1714)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1714
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_213082"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1313)
                     /locus_tag="VEA63_06835"
     CDS             complement(<1..1313)
                     /locus_tag="VEA63_06835"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HYD83748.1"
                     /translation="MPTPLLEARHLTKKFPGVVALRDVAFDLRAGEVHALCGENGAGK
                     STLIKLLSGIHPHGSYEGELRVDGRAVQFRHIRDAEAAGIAVITQEFALVDELSIAEN
                     IFLGREPRRGVRIDWLEMNRRAAVLLADFGLTLAPETPVRELGIGHKQLLEIVRAMDK
                     QTRVLVLDEPTAALTEQEVSVLIDHLRRLRAQGTACIYISHKLDEVFAIADRITVLRD
                     GATIATLPTAAASAPLIIKHMVGREIADLFPRKNDHAPGDVQLSVRELSVAPAKHAAP
                     FLRDISFDLRAGEVLGFGGLMGAGRTELLMHLFGAWGRRIHGDVTLHGASYSTPNPRA
                     SIRRGLVLVSEDRRRYGLVLDEDISFNLSLSSLHRFVARTGQINQPAEHRRNQQFFDS
                     LRIKAPHQHARVGGLSGGNQQKVVLGKALMTEPTVIMLDEPTRGI"
     gene            complement(1371..>1714)
                     /locus_tag="VEA63_06840"
     CDS             complement(1371..>1714)
                     /locus_tag="VEA63_06840"
                     /inference="COORDINATES: protein motif:HMM:NF024799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HYD83749.1"
                     /translation="NDGTAGGAIQSLVEEGLAGKVLVTGQDADLAACQRILRGTQAMT
                     VYKPLKTLASNAARIAVEVAQGNRPTTTATLDNGAKQVPSIFENVIAVDKDNLESTVI
                     ADGFHKASALK"
BASE COUNT          288 a          439 c          658 g          329 t
ORIGIN      
        1 tcgatgccgc gcgtcggctc gtcgagcatg atgacggtcg gctcggtcat gagggctttg
       61 ccgagaacga ccttttgctg attgccgcca gagagtccgc ccacgcgcgc gtgctggtgg
      121 ggagctttga tgcgaagcga atcgaagaac tgttggttgc gccggtgctc ggccggttga
      181 ttgatctggc cggtgcgcgc gacgaaacgg tggagcgaag agagggaaag gttgaacgag
      241 atgtcttcgt ccaaaacgag gccgtagcgg cggcggtctt cgctgacgag cacgagacca
      301 cggcggatcg aggcgcgggg attcggcgtg gagtaagacg cgccgtgaag cgtcacatcg
      361 ccgtggatac ggcggcccca ggcgccgaaa agatgcatga gcaactcggt gcgaccggcg
      421 cccatgaggc cgccgaagcc gagcacttcg ccggcgcgca gatcgaagct gatgtcgcga
      481 agaaacggtg ctgcgtgttt tgcgggagcg acactgagtt cgcggacgga aagttgaacg
      541 tcgccggggg catggtcgtt tttgcgggga aagagatctg cgatttcgcg tccgaccatg
      601 tgtttgataa taagaggggc gctcgcggcg gcggtcggga gcgtcgcgat tgtggcaccg
      661 tcgcgcagca cggtgatgcg gtcggcgatg gcgaaaactt cgtcgagttt gtgtgagatg
      721 tagatgcagg cggtgccttg ggcgcggagg cggcggagat gatcgattag aacggagacc
      781 tcctgctcgg tgagcgcggc ggtgggttcg tcgaggacga ggacgcgcgt ttgcttgtcc
      841 atggcgcgga cgatctcgag caattgtttg tggccgattc cgagttcgcg cacgggggtt
      901 tcgggcgcga gcgtcaggcc gaaatccgcc aggagcacgg cggcgcggcg gttcatttcg
      961 agccagtcga tgcggacgcc gcggcggggt tcacggccga ggaaaatgtt ttcggcgatg
     1021 ctgagctcgt cgacgagcgc gaactcttgc gtgatgactg cgatgccggc ggcttcggcg
     1081 tcgcggatgt ggcggaactg cacggcgcgg ccgtcgacgc gcagctcacc ttcgtaactt
     1141 ccgtgcggat gaatgccgga gaggagtttg atcagcgtcg atttgcccgc gccgttttcg
     1201 ccgcacagcg cgtgaacttc gccggcgcgc agatcgaaag ccacgtcgcg aagggcgact
     1261 acgccaggaa actttttcgt aaggtggcgg gcttcgagga gcggggtggg cattcgaggg
     1321 ccggtgaaga cggggcggaa gctggcgatg gggaggagcg gcgttccccg ttacttcaag
     1381 gcggaagctt tatgaaagcc gtcggcgatg acggtgctct caaggttgtc tttgtcgacc
     1441 gcgatgacgt tttcgaaaat cgaggggact tgtttggcgc cattgtcgag cgtcgcggtg
     1501 gtggtcgggc ggttgccttg ggcgacttcg acggcgatcc gcgcggcgtt gctggcgagg
     1561 gttttcaggg gtttgtagac ggtcatcgct tgtgtgccgc ggagaatacg ttggcaggcg
     1621 gcgaggtcgg cgtcctgacc ggtgacgagc actttgccag cgaggccttc ctcgacgagc
     1681 gactggatgg cgccgccggc ggtgccgtcg ttgg
//
LOCUS       DASPLT010000661         4918 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_31758, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000661 DASPLT010000000
VERSION     DASPLT010000661.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 4918)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4918)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4918
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_31758"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            566..880
                     /locus_tag="VEA63_06845"
     CDS             566..880
                     /locus_tag="VEA63_06845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD83750.1"
                     /translation="MLSFALTGSIGSVLATAPDELTLAISSVLDRVGEPPRVVWILCV
                     ARDSALRAKILVHLGEGDPVRIEGEIEQRRRQVGELAFHSVGFVIHSIERLPSPSEGA
                     AP"
     gene            877..1182
                     /locus_tag="VEA63_06850"
     CDS             877..1182
                     /locus_tag="VEA63_06850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD83751.1"
                     /translation="MRRLYLTPREYNALLEAQGGKCCVRGCRATEDLIAEHSTPNALK
                     PGKPDQLMCSACHKVKTLRDVKAIAKVKRLNGKTMSQYERRKKFGSRLRGRGFDKRE"
     gene            complement(1397..2728)
                     /locus_tag="VEA63_06855"
     CDS             complement(1397..2728)
                     /locus_tag="VEA63_06855"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HYD83752.1"
                     /translation="MLLVFWIFCAASAGAHADEIQPSDSVVTGVVIRAAPTTSSDRLG
                     LLRPEETLLFVSDRPGWNEVSLRDGRTGFVSKRWTIRIESEEPGEDPFPITEAAPAMF
                     AHFVYVGQGAGAILEFPCGVAVIDTGGQYTGDPDGGESFNTYLDGFFAAHPQYQNTID
                     VLFTSHPHLDHLAGLGGLLVNGALRYRVRNVVDNGQSGSGDSVRRQMNYRVISRARGA
                     GFNGVSVTSALIPNGVTNPVIDPIPRIDPEYPIRCEGQGVDPTFRVFWGGWERDTVRE
                     LGGRGRYHSKPNNHSLVIRIDYGEASFLFTGDLETPAIEDMLTLYQDHLSNFDVDVYH
                     VGHHGADNATTAPFLAAMSPQMAIISMGDLVDTTSASAPGHGHPRVAALRLLQNAVSG
                     ARIAPVTEQAFETEHDLVDVRIDRAIYATGWQHDIVLRADARGDFSFDHVH"
     gene            2865..4481
                     /locus_tag="VEA63_06860"
     CDS             2865..4481
                     /locus_tag="VEA63_06860"
                     /inference="COORDINATES: protein motif:HMM:NF025056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIR domain-containing protein"
                     /protein_id="HYD83753.1"
                     /translation="MGKVFISYAHEDEEKARKIKARLDDLGFETWIDRSAPASRDDIS
                     TVIDTKLSAVDCVLVLWSADSRGSVWVRGEALKGLESNKYLGLLLTAFVPPVPFNALA
                     APDLSDWTGLHLHFGWDALVRGLADMSKAKSAILNKHTGYQKEESDHADRLANKQERL
                     PLEEPAEPLDVGPAQGDIDAIIAATRAILGRSAKLDLTASIAQAALDQEMLRDATFVV
                     STAGNVPRPTNARFEVETSIGAALKRAHDGDLIVIDRGRYEECLRVTKRVRIVGLGAG
                     ADRPIIAAYTEQPTVVLTESARLENISVETRYRSHAIQCDRGQPAILRCDIRRFADAS
                     DDKDAAFYVAGKANPIVIATSIASTGCRALYFVVNSGGQFLGVNVVALRGDGMTCRGR
                     PNFHACTIEAIGGHAVNVFTSGAPTFESCDISGRGAAVIKASNRAYPRITESRVNAIR
                     QLAFDFEDDASGRYEGNIVSIETDESAGTGRKSEEGFFARFKSKQDAIVRRAHGSEFA
                     RLKGRGKPLFIANVTPTGEDLPEPVSFGGF"
BASE COUNT          970 a         1524 c         1529 g          895 t
ORIGIN      
        1 ctttgcgtcg gcggcagccc agtccgacct gcatcgatca gcgcactgaa caacgcgtca
       61 cccgccggac cgcactgcgg cacgcagagc tgtcccccgc tttgatcgcg tgtcgcaacg
      121 cgggcgggcg cgcgcaaggg acgagccttc ggcgcgtacc gcgcgcccct gcccagcgcc
      181 ccccttctcg cgtgcaggcc gctttcagca tcgcggggaa cccctgagcg acaccggcga
      241 aagcagctct cgcccaactg aatgcgcgcc tgcaagctgc ggcgcttgat cgccaagcgc
      301 gtcctacgcg cgccccgctg agccgcccac gacgggtggc gcgtccgaag cggtcggccc
      361 tctctcacga ccgttctgcg cttcgcgaac ctcttcccgc tccttcattg ctcgcacgcg
      421 gtcggacggg cgcaaggcca agcctgcggg cgcgctgacg cgcgcgcctt gcgcccgacc
      481 tcttcccgcg tgcgctcggg tgttggcgtc gcggggagtc ggcccgcggc gcgccgccgg
      541 cgcgtaccgg cccgagcttt tcctcatgct ttcctttgcg cttaccgggt cgatcggctc
      601 cgtgctcgca accgcgcccg atgaactcac cttggcgatc tcatcagtgc tcgaccgcgt
      661 cggcgagcct ccgcgcgttg tatggatcct ctgcgtggcg cgcgacagcg ccctacgcgc
      721 caagatcctt gtgcatctgg gcgagggcga ccccgtccgc atcgagggcg agatcgagca
      781 gcgccggcgc caagtcggcg agcttgcctt tcatagcgtg ggctttgtca ttcattcgat
      841 cgagcgcctg ccctcccctt cggaaggagc cgcgccatga gacggctcta cctgacaccg
      901 cgggaataca atgcgctgct cgaggctcag ggcggcaagt gctgcgtgcg aggctgcaga
      961 gcgacagaag acctgatcgc cgaacattcg acccccaacg cgctaaagcc aggaaagccc
     1021 gatcagctga tgtgcagcgc ctgccacaag gtgaagacgc tccgagacgt caaagccatc
     1081 gccaaggtga agcgactcaa tggcaagacc atgagccaat atgagaggcg gaagaaattt
     1141 ggatcgaggc tgcggggcag aggcttcgac aagcgggagt gatgaggcgt cgcggcgcgc
     1201 ttgatgcgcg ccgccgggct ctcgtcgctg cgcgaccgag ccgcggcatg cccgcgtctc
     1261 ggccctcgcg ggcttcgatc cctgacgcgg gaggcttccg cattatccca ggagcggact
     1321 ctggctcgac ccttcctgga agcggacgcc ggctgtcagc tcaccacgct ctcacctgca
     1381 tcgaccgcta tgccgatcag tgcacgtggt cgaaagagaa atcgccgcgc gcatcggcgc
     1441 gcaaaacgat gtcatgctgc cagcccgtgg cgtagatggc gcgatcgatc cggacatcta
     1501 ccaaatcgtg ctcagtctcg aacgcctgct cagttaccgg tgcgatgcgc gctcctgaca
     1561 cggcgttttg cagcagccgc agtgccgcga cgcgcggatg gccatgaccg ggggcgctgg
     1621 cgcttgtcgt gtcgacgaga tcgcccattg aaatgatagc catctgtgga ctcatcgccg
     1681 ccagaaacgg cgcggtggtg gcgttgtcgg cgccatgatg gccgacgtga tagacgtcga
     1741 cgtcaaaatt gctgaggtga tcttggtaga gcgtaagcat gtcttcgatt gccggcgttt
     1801 caagatcgcc ggtgaacagg aaagacgcct ctccgtaatc gatacggatc accaagctgt
     1861 gattgttggg cttgctgtga tagcgcccgc ggccgccaag ctcccgaaca gtatcccgct
     1921 cccagccacc ccaaaatacg cggaacgtgg gatcaacgcc ctgcccctcg caccggatcg
     1981 gatactcggg gtcaatacgg ggaatgggat cgatcaccgg attggtgaca ccatttggga
     2041 taagcgctga cgtgaccgag acgccattga acccggcgcc cctagcgcgt gagatgacgc
     2101 gataattcat ctgccgacgg acggagtctc cagatcctga ttggccgtta tcgacaacgt
     2161 tgcgaacccg gtagcgcaac gcgccattaa cgagaaggcc tccaaggcca gcgagatggt
     2221 cgaggtgtgg gtggctggtg aacaggacgt cgatggtgtt ctggtattgc gggtgcgcgg
     2281 cgaagaagcc gtcgagatag gtattgaaag actccccgcc gtctggatcg ccagtatact
     2341 gcccgccggt atcgatcacg gcgacaccgc agggaaactc aaggatcgcg cctgcgcctt
     2401 ggccgacata gacgaagtgc gcgaacatgg caggcgcagc ttccgtgatc ggaaatggat
     2461 cttcacccgg ctcctcgctc tcgatcctga tcgtccaacg cttcgaaaca aagccagtgc
     2521 ggccatcgcg cagggatact tcgttccagc cgggacgatc gctcacgaac aatagtgtct
     2581 cttcggggcg gaggagaccg aggcgatcgg aggatgtcgt gggcgcagcg cgaatgacga
     2641 cgcccgtgac gacgctgtcg ctgggctgaa tctcatcggc gtgtgcgcct gctgaggccg
     2701 cgcagaagat ccaaaaaaca agaagcatga tgcgcatttg agccccgcct actctgcccg
     2761 cgcggctggc atgcctcgcg catagttcga aatcatcgca cgccgcgtta accaacactt
     2821 gcggagcgca ctggaggttg taccgtcccg ccgggagttg ggcgatgggc aaagttttca
     2881 tctcgtacgc gcacgaggac gaagaaaaag cgcgcaagat caaagcgcga ttggacgatc
     2941 tagggtttga gacatggatt gaccgatcgg ctcctgcgtc tcgcgacgac atcagcacgg
     3001 tgattgacac caaactgagc gccgtagact gcgtcctcgt tttgtggtcg gcggattctc
     3061 ggggttctgt ttgggtgcga ggcgaagcgc ttaaagggct cgaatccaac aagtacctag
     3121 gtcttctgct cactgcattc gtaccacctg tgccattcaa tgctttagcc gcgccagacc
     3181 tctcagattg gactggactg cacttgcact ttggctggga cgctctggtg cgcggcttgg
     3241 ccgacatgag caaagccaag tcggccatcc tgaacaagca caccggctat caaaaagaag
     3301 aatccgacca cgctgaccgt ctcgcgaaca agcaagagcg cttgccgctg gaagagccgg
     3361 ccgaaccatt ggacgtaggg ccggcgcaag gcgatatcga cgcaatcatc gcggcgacaa
     3421 gagcgatcct cggacgttcg gcgaagctcg atctcaccgc ctcaattgcc caagcggcgc
     3481 tcgatcagga aatgctccgt gacgcgacat tcgttgtaag caccgccggc aacgttcctc
     3541 ggccaacaaa tgcgagattt gaggttgaga catctatagg cgccgcgtta aagcgggcgc
     3601 atgacggcga tctcattgtg attgaccggg gccgctacga ggaatgcctg cgcgtaacga
     3661 aacgtgtgcg cattgttggc ctcggcgctg gcgccgatcg cccaatcatc gcggcctaca
     3721 ccgagcaacc cactgtggtg cttacggaaa gcgcgcggct tgagaacatc tccgttgaga
     3781 cgcgctaccg cagtcacgcc attcagtgcg atcgagggca accggcaatc ctccggtgcg
     3841 acatccgccg ctttgccgac gcttccgatg acaaggacgc ggcgttctat gtggcgggca
     3901 aagccaatcc gatcgtcatc gcaacaagca tcgcatcaac aggatgccgg gcattgtact
     3961 ttgtcgtgaa ctccggcggt cagttcttgg gcgtgaacgt cgttgccctc aggggcgacg
     4021 gaatgacctg ccgcgggcgc cccaactttc atgcctgcac catcgaggcg attgggggcc
     4081 acgcggtaaa cgtcttcacc tccggtgctc ctacatttga aagctgcgat atttcggggc
     4141 gtggcgccgc tgtgattaaa gcaagcaatc gagcttatcc gcggatcacg gaaagtcggg
     4201 tcaacgcgat taggcaattg gcgttcgatt tcgaggacga cgcgtctggg cgttatgaag
     4261 gcaacatcgt ctcaatagag accgacgaga gtgccggcac gggaagaaaa tccgaagaag
     4321 ggttcttcgc gcgcttcaag tcgaagcagg atgccatcgt gcgcagagcc catggtagtg
     4381 agtttgcaag gctcaaggga cgcggcaagc cccttttcat cgcgaacgta acaccgacgg
     4441 gagaagatct gcccgaaccc gtgtctttcg gcgggttcta aaggcgagtt tgctgcttca
     4501 gcccaggaac ggacactagc tataagcgcg ccaagagcgg acgttcacgt gtcaccttgc
     4561 cgttggatac ggtcacctct cgtcagcgcc tttcgtatca tcacccggtg aaacgtcggc
     4621 gtcagcggcg gcgtcctcta gcgagggccg atcgcgtgcg gtttgcacac gatttggttc
     4681 tcgcggttcg gcctgcacga ccgcaggagc ttccgcgcgg gacggcaata ttccttcggc
     4741 gacgaagaat gccaccgcta ggttcacgaa aagcaagccg gcaaaaaggg cgaagttgct
     4801 gactggctcc caatatcgcg tcaagccgcg cagtccactg ggcgcgagtg aaggcgttgg
     4861 ctcaaagccg tggagttgcc gcggtcacgc catgcgcgcc gcgtcgcgtg tctgacgc
//
LOCUS       DASPLT010000662         1048 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_109690, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000662 DASPLT010000000
VERSION     DASPLT010000662.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1048)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1048)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1048
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_109690"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..626)
                     /locus_tag="VEA63_06865"
     CDS             complement(<1..626)
                     /locus_tag="VEA63_06865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD83754.1"
                     /translation="MKNKVAKRSVGSQSEVTDDTAAINDAIRAARAEITLADRELTFA
                     ESSLEREERAAQDGDRWKCAPDRDTLAEADAADRGDVEFFGKRAYGDVWHIMKARGHV
                     PNIETYRRQNPICRARARVRELQARRAEYQRRQDELCNTLASMCRSKERVRTRVTART
                     VHVVPQASTRHGRSRRPVRVAQKKTVGKAAADPDGPPRPRTSSSGGAW"
     gene            complement(694..852)
                     /locus_tag="VEA63_06870"
     CDS             complement(694..852)
                     /locus_tag="VEA63_06870"
                     /inference="COORDINATES: protein motif:HMM:NF024803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HYD83755.1"
                     /translation="MLTIGDVAKALGLSTARVNQLDAVLRPVRRENGHRRYAAADVER
                     LMKERSKR"
BASE COUNT          154 a          339 c          341 g          214 t
ORIGIN      
        1 caccacgcac cccccgagct ggaggtacgc ggtctaggtg ggccgtcggg gtcggcggcg
       61 gctttgccta cggttttctt ctgcgccacg cgcacggggc ggcgcgagcg tccgtgccgc
      121 gtcgatgcct gcggtacgac atgcaccgtc cgtgcggtga cacgcgtgcg cacgcgctcc
      181 ttgctgcggc acatcgaagc gagcgtgttg cacagctcgt cttgacggcg ctggtactca
      241 gcgcgacgag cctgtagctc acgcacgcgg gcgcgtgcac ggcagatcgg gttctgtcgg
      301 cgatacgtct cgatgttcgg gacgtggccg cgcgctttca tgatgtgcca gacgtcgcca
      361 taggcgcgct tgccgaagaa ctcgacatca ccgcgatcgg cggcgtccgc ctcggcgagc
      421 gtgtcgcgat cgggtgcgca cttccatcgg tcaccgtctt gcgccgcgcg ctcctcgcgt
      481 tcgagcgagg actccgcgaa ggtcagttcg cgatcggcga gggtgatctc agcgcgggcg
      541 gcgcggatgg cgtcgttgat cgcggcggtg tcgtcggtga cttcgctctg gctaccgaca
      601 gagcgctttg ctactttgtt cttcatcggg tcaggtccca tctggctcgt tcaaggcccc
      661 gggcgtttgc ggcgctgcgg ggctttcatt tgcctagcgc ttgctgcgct ccttcatgag
      721 tcgctcgaca tcagcagcgg cgtagcgtcg atgaccgttc tcgcgtcgaa ccgggcgcag
      781 cacggcatcg agctgattca cgcgcgccgt agaaagcccg agcgccttag cgacatcgcc
      841 gatcgtcaac atgtggctat cgaacttcat gactcattga ccgttagcag atgccgtttt
      901 cgcatgtcga tatcattcga cgtgatcacg ctagatttat cgactcccgg taggtccccc
      961 actaaacacg ggtccggagt ccttcgccgc cgccgagact ccgtgcccgg agcgtggcca
     1021 gccttagcgg aatcagacgt aacgcgac
//
LOCUS       DASPLT010000663         2278 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_253909, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000663 DASPLT010000000
VERSION     DASPLT010000663.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 2278)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2278)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2278
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_253909"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..585)
                     /locus_tag="VEA63_06875"
     CDS             complement(<1..585)
                     /locus_tag="VEA63_06875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HYD83756.1"
                     /translation="MNVAEVTAKAKVLLEALPYIQDFRGSTFVVKYGGSFMDDPDPIA
                     RTRVAYDIAFFAAVGINIVVVHGGGKAITRAMETSGLKANFVNGLRVTDEATIAVVKK
                     TLDEIVNKDVCDAIVSAKGKPKGLPGDTVLVCQKLTVDDNGNEIDLGYVGDVTEVKVK
                     LIKKEIAEGFVPVISPVAEGYDGKPYNVNADLVAG"
     gene            846..2162
                     /locus_tag="VEA63_06880"
     CDS             846..2162
                     /locus_tag="VEA63_06880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009513410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ornithine
                     acetyltransferase/N-acetylglutamate synthase"
                     /protein_id="HYD83757.1"
                     /translation="MPSTNLTFTSRDAHRNWLASQAELPAGFRVGTTRFEFTPREAPK
                     PAKMTLTLLALDQPSRDFAAVFTRNAFPGAPVIVGRQRLREETLGAIIVNNKISNVCA
                     PGGVETAEKICLATARLLGLSSGQVLPSSTGVIGWTLPSDAMIAALPQAVGALSAGSI
                     MPAAEGIVTTDLYPKVRRAVVGKGSIVGIAKGAGMIEPNLATMLVYILTDIAVPRAEL
                     RAMLVRAVDPSFNAISIDSDTSTSDTVVAISSGRVTGVDLKAFEAALTLVCRDLAEDV
                     VRNGEGVRHVIRVAVKNAATPQLARSLGKVIVNAPLFKCAVAGNDPNVGRLVQAIGKH
                     VGASAPETDLSKLRLRLGGIEIFAGGVFQLNPEKENALVEHLRGAELYASAPPKDGVF
                     APPIDYPPHERCVEIDVDLGSGTHAATIFGGDLTHEYVSENADYRS"
BASE COUNT          423 a          768 c          668 g          419 t
ORIGIN      
        1 acccgccacg aggtctgcat tcacgttgta aggcttgccg tcgtagcctt ccgccaccgg
       61 cgagatcacc ggcacgaagc cttccgcgat ctccttcttg atcagcttca ccttcacctc
      121 ggtgacatcg cccacgtagc ccaaatcgat ctcgttgccg ttgtcgtcga ccgtgagctt
      181 ttggcaaacg agcaccgtgt cgccgggcag gcctttcggt ttgcccttcg cgctcacgat
      241 cgcatcgcag acgtctttgt tcacgatctc gtcgagggtc tttttcacga ccgcgatcgt
      301 cgcctcatcg gtgacgcgca ggccattgac gaaattcgcc ttcaagcccg acgtctccat
      361 cgcgcgcgtg atcgcctttc cgccgccgtg cacgacgacg atgttgatgc ccacggccgc
      421 gaaaaacgcg atgtcgtagg cgacccgcgt gcgcgctatc ggatcgggat cgtccatgaa
      481 actcccgccg tatttcacga cgaacgtgga gccgcgaaaa tcctgaatgt agggaagcgc
      541 ctcgaggagc accttcgcct tggctgtaac ttcagcaacg ttcatcagcg atcaaaaccc
      601 gcggcacaaa gccgcacggt ggtggtggaa ggagtgagcg ttcacttgtt ggtgcgaagt
      661 ggggaaaggc attacgcagc cgctcgccgc atttccacca aattcttgga gcaaatggaa
      721 tgtcggggaa agcacccgcc gtgcccgcac gatttctccc attgccgccg agcgcattgc
      781 ggtcgttgcg aagtcgtcgc ataattcgac ttcactccgc cgcgacgccc gcccacttgt
      841 tgcacgtgcc cagcacaaat ctcactttca cttcgcgcga cgcacatcgc aactggctcg
      901 cttcgcaagc cgagttgccc gccggttttc gcgtcggcac gactcgcttc gagttcactc
      961 cgcgcgaggc gccgaagccc gcgaaaatga cgctcacgct cctcgcgctc gaccaaccgt
     1021 cgcgcgactt cgcggcggtc ttcacccgca acgcgtttcc cggcgctccc gtgatcgtcg
     1081 gccgccagcg gctgcgcgaa gagacgctcg gcgcgatcat cgtgaacaac aaaatctcca
     1141 acgtctgcgc gcccggcggc gtcgagaccg cggaaaaaat ctgcctcgcg accgcgcgcc
     1201 ttctcggcct ctcgagcggc caggtgttgc cgagttcgac cggcgtgatc ggttggacgc
     1261 ttccgtccga cgcgatgatc gctgcgctgc cgcaagccgt cggcgccttg tccgccggct
     1321 cgatcatgcc cgccgccgaa ggcatcgtga ccaccgatct ttatcccaaa gtgcgccgcg
     1381 ccgtcgtcgg caaaggcagc atcgtcggca tcgccaaagg tgccggcatg atcgagccga
     1441 atctcgcgac gatgctcgtc tacatcctga ccgacatcgc ggtgccgcgc gccgaactgc
     1501 gcgcgatgct cgtgcgcgcg gtcgacccga gtttcaacgc gatcagcatc gatagcgata
     1561 cgagcacgtc cgacaccgtt gtcgcgatct cgtcgggacg ggtcaccggc gtagacctca
     1621 aggcgttcga agccgcgctc acgctcgtgt gccgcgacct cgccgaggat gtcgtgcgca
     1681 atggcgaagg tgtgcgtcac gtaattcgcg tcgccgtgaa aaacgcggcg acgccgcaac
     1741 tcgcgcggtc gctcggcaag gtgatcgtca acgcgccgct gttcaaatgc gccgtcgccg
     1801 gtaacgatcc caacgtcggc cgcctcgtgc aggcgatcgg caaacacgtc ggcgcgtccg
     1861 cgcctgagac cgatttatcg aaactgcggc tgcgcctcgg cggcatcgag atcttcgccg
     1921 gcggggtgtt tcagttgaat cccgaaaaag aaaacgcgct cgtcgagcac ctgcgcggcg
     1981 cggagctcta cgcgagcgcg ccgccgaaag acggcgtgtt cgcgccgccg atcgattatc
     2041 cgccgcacga gcgctgcgtg gaaatcgacg tcgatctcgg cagcggcacg catgcggcga
     2101 cgatcttcgg cggcgatctc acgcacgaat acgtgagcga gaacgccgac taccgaagct
     2161 gagcgcggcg ccgccgcgga agttccaagc accaagatcc aagctcccaa ataaaacccc
     2221 aaacaccaaa tcagaacagg tgtttggaga ttggagtttc tctggagctt ggtgcttg
//
LOCUS       DASPLT010000664         1134 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_193098, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000664 DASPLT010000000
VERSION     DASPLT010000664.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1134)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1134)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1134
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_193098"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..318)
                     /locus_tag="VEA63_06885"
     CDS             complement(<1..318)
                     /locus_tag="VEA63_06885"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HYD83758.1"
                     /translation="MYIAKLIRSLFAAAVFLGVLSAGYAQVVSSGITGTVVADDGRPI
                     AGASVTVRHEPTNTVSTAVTRDNGRFSFRGLPVGGPFTVSATAEGATIGSVSGVTTLL
                     GEDV"
     gene            638..>1134
                     /locus_tag="VEA63_06890"
     CDS             638..>1134
                     /locus_tag="VEA63_06890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:dicarboxylase symporter family
                     transporter"
                     /protein_id="HYD83759.1"
                     /translation="MLGLAIGVVAGAITLVIGAFYPPALTFMRAVSTHVLDPFGQIFL
                     RMLFFVVIPLVFASLASGVTQLGRLDRLGPLAVRTFGLFALNMIIGVALGLLMMNVLQ
                     PGDYLAEEAKNRLMAEYGSVAQQTVQTQAERAPLTAMTIVEMFMPKNLFGAFVGNSRG
                     SLGDVL"
BASE COUNT          223 a          363 c          341 g          207 t
ORIGIN      
        1 gacgtcttcg ccgaggaggg tcgtgacccc gctcaccgat ccgatcgtcg caccttccgc
       61 cgtggcggaa acggtgaacg gccctccgac gggaagtcca cggaacgaga aacgaccgtt
      121 gtcgcgtgtg accgcggtcg aaaccgtatt ggtcggctcg tgacgaaccg tgacagatgc
      181 tccagcgatc ggccggccgt cgtcggcgac gaccgtgccg gtgataccgg acgatacgac
      241 ttgcgcatag ccagcggata aaacgccgag aaagacggcg gcggcgaaca gcgacctgat
      301 gagtttagcg atatacatgt gtgtgcttag gttcaaagga gagaacttcc cagcaatcgg
      361 gcgaacttcc cctttccgcg tagcaagagc cgtgcgaaac ctcagtcaag cccccaaccc
      421 ccgtcaaaaa gcgtgatgcg caatcgtaac gaaataggca atttgccgta acgcgcctct
      481 cccaagagca ggactaccga gtcagccgac ccgccggcca ctgcccacgc ttcgaccgcg
      541 cgccccagcc acacttgccg ttggacaggg cggcggtgga ctctcagcgt ttccgcccaa
      601 tatgggcgaa atcaaccagc ggctggcaaa tcggatcttg ttgggcctcg caatcggcgt
      661 cgtcgccggc gcgatcaccc tcgtcatcgg agcattttat ccgcccgcgc tgacgttcat
      721 gcgagcggtg tccacacacg tgctcgatcc cttcggacag attttcctgc gaatgctctt
      781 cttcgtggtg atcccgctcg tgtttgcatc gctcgcgtcc ggcgtcaccc aactgggccg
      841 gctcgaccgg ctcgggccgc tcgccgtgcg gaccttcggt ttgttcgcgc tcaacatgat
      901 catcggcgtc gcgctcgggc tgctcatgat gaacgtgctg cagccgggcg attatctcgc
      961 cgaagaggcg aagaaccggc tcatggcgga atacggcagc gtggcgcaac aaaccgttca
     1021 gacgcaagcc gagcgagcgc cgctgacggc gatgacgatc gtcgagatgt tcatgccgaa
     1081 aaatcttttc ggcgcattcg tgggcaacag ccgcggctcg ctcggcgatg tgct
//
LOCUS       DASPLT010000665         1169 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_151241, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000665 DASPLT010000000
VERSION     DASPLT010000665.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1169)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1169)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1169
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_151241"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            540..>1169
                     /locus_tag="VEA63_06895"
     CDS             540..>1169
                     /locus_tag="VEA63_06895"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HYD83760.1"
                     /translation="MTTDAPPSRERDASLVESEGWCIHTAHEIGSTNSAAARLPAWHA
                     VRARVQTDGRGRTGRAWTSDEGGLWLSAVLPCTGNRARWAILPLAAGWAIVAALKELG
                     AHGLRLRWPNDIMAGHRKLAGLLVERYRNDTAVVGIGLNVFNSPEEAEPALAGATVRL
                     ADLVSGGYTLDDLTRVILRSISRAHAMIRNDEFRLIADELNVQWSRPRLV"
BASE COUNT          199 a          434 c          317 g          219 t
ORIGIN      
        1 gctccctctt ttgacggacc cgtccatccg actctgaccg tcgcgggcca gcttctggct
       61 ctcggcggtt tgtatatcat ctgccacggc atccgccgcc ggattcaagc catccagtcg
      121 accgaaacgc cgtcacgcga aaatcctctg agctaacacc gaacaattca tttgaaccac
      181 gaatgaacac gaatgaaatc gcgttcgaat gcggaatcat atcggaacgg cgaagagtga
      241 ggtaacgatc ggcctcagcg ctgtccttcg gctcccctga attcgcgttc attcgtgtca
      301 attcgtggtt tttcttctgc acgcccggct aagacgcctc ggctcatccc atcacgatcg
      361 tccgcgccat cgagccgtgc ccgcgtcccg agcctgtttg tcatctaata gatgacaaac
      421 ccgttggtcg ttcgcgggtg tttgcgcggc acacgcgtag cttccgcgga tcagctttcg
      481 ctcaacaatt ggcaacccat gcgcagcccc gcacccgccg cgctgttaag ataaaggcga
      541 tgaccaccga cgcaccgcct tcccgcgaac gcgacgcttc gctcgtcgag agcgagggct
      601 ggtgcattca cacggcgcac gaaatcggct ccaccaactc cgccgcggcc cgcctccccg
      661 cctggcacgc cgtgcgcgcg cgggtgcaaa ccgacggccg cggccgcacc ggtcgcgcgt
      721 ggacctccga cgagggcggg ctctggcttt ccgcggtgct gccttgcacc ggcaaccgcg
      781 cccgctgggc cattctcccg ctcgccgccg gttgggccat cgtcgccgcg ctgaaagaac
      841 tcggcgctca cggcctgcgt ctccgctggc ccaacgacat catggccggc catcgcaaac
      901 tcgccggtct gctcgtcgaa cgctaccgca acgacaccgc cgtcgtcggc atcggcctca
      961 acgtttttaa ttctcccgag gaagccgagc ccgcgctcgc cggcgccacg gtccgcctcg
     1021 ccgatctcgt ctccggcggc tacacgctcg acgatctcac ccgcgtcatt ctccgctcga
     1081 tcagccgcgc ccatgcgatg attcgcaacg acgagttccg cctcatcgcc gacgagttga
     1141 acgtgcaatg gtcccgaccg cgcctcgtc
//
LOCUS       DASPLT010000666         2742 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_68154, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000666 DASPLT010000000
VERSION     DASPLT010000666.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 2742)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2742)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2742
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_68154"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(151..696)
                     /locus_tag="VEA63_06900"
     CDS             complement(151..696)
                     /locus_tag="VEA63_06900"
                     /inference="COORDINATES: protein motif:HMM:NF025013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4142 domain-containing protein"
                     /protein_id="HYD83761.1"
                     /translation="MSLCTALSAQSSTNTTSSTRTSDSTAADRRTELATDTRAAANLS
                     RSDRNFFEKAAKSGMKEVAVSRSTLDRLVNPQVKHFAQMMVDDHSKANAELMALAQKK
                     GVTLPPKDDMKLMEKWSKKTDEVDEDYMEEMVDDHQEAVELFEEASKSEDAEIAAFAN
                     KTLPTLRQHLTVAKDLKKAVD"
     gene            complement(860..>2742)
                     /locus_tag="VEA63_06905"
     CDS             complement(860..>2742)
                     /locus_tag="VEA63_06905"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M13 family metallopeptidase"
                     /protein_id="HYD83762.1"
                     /translation="GKWYAGTKIPADKSRWGGFNELAERNWAALRTIVEEAAAAPGEA
                     GSVRQKVGDFFASAIDTATINARGLKPIEDELAAIEAVKSVDELVRVATQIHLRVGAP
                     FFNAAFYPDQKQSDIYGFYLSQGGMSLPSKEYYFSEKFARERWELVGHIAKMLELAGE
                     TKAAAFQHAETIMAIERTLAENAKLPVDLRDRVANYNKFSIDEAAAEFPGFPLKLFVA
                     EMQIPAAVKDVIVGQPKFFEGLSKLLAERPLEELKVYTRWQLLHAVAPYLSEPFEQEN
                     FRFYGTVLNGTPEQEPRWQRATKAVDGAIGEALGQLYVEKHFPPAAKARMMEMIDNIK
                     AVMRSRLEKVEWMTEPTRQKALAKFDRFEPMIGYPDTWRDYASVEVKRDDYFGNVWRA
                     AYFEARRLIDRTGQKVDKREWGMTPPTVNAYFSPVTNQIVFPAGILQPPFFDTRLDDA
                     VNYGAIGAVIGHEITHGFDDQGRRYDADGNLVDWWTEEDATRFRERAQLVVEQFNNYQ
                     ALPGLAINGQLALGENIADLGGTSIAFEALQRSLEGKPVPPKIDGFTAEQRFFISWAQ
                     QWRTAYREDAMRLQVARGPHAPGNFRAFGPLVNLDSFYAAFEIKEGDAMWRPPEKRAK
                     IW"
BASE COUNT          491 a          964 c          727 g          560 t
ORIGIN      
        1 gacggtatcc gcggccgctt tcgactggtt ttcgatgaac aaaacaaatt cataagcatc
       61 gaggccgcgc cgctcgcttg agtcggtcga aaaaaagcgg agccggttcg gctccgctga
      121 ttgagtcctg tcgttggctc ggtgcgcgat tcagtcgacg gccttcttca gatccttggc
      181 caccgtgagg tgctgacgca gcgtcggcag cgtcttgttc gcgaaagccg cgatctcggc
      241 gtcctcgctc tttgaagctt cctcgaagag ttccacggct tcctgatggt cgtcgaccat
      301 ctcctccatg tagtcttcgt cgacctcgtc ggttttcttc gaccattttt ccatcagctt
      361 catgtcgtct ttcggcggca gcgtgacgcc tttcttctgc gccagcgcca tcaactcggc
      421 gttcgccttc gaatggtcgt ccaccatcat ctgcgcgaag tgtttcacct gcggattcac
      481 cagacgatcg agggtcgaac gcgacaccgc gacttccttc atgccggatt tcgccgcctt
      541 ctcgaagaag ttgcggtcac tccggctcaa gttcgccgcc gcacgcgtat cggttgccaa
      601 ctccgtccgg cgatcggcgg ccgtcgaatc gctcgtgcgg gtggaagaag tagtattcgt
      661 cgacgattgc gccgacaacg cggtgcatag cgacaccgcg gaaacaagga gcgcgcaacg
      721 aagcgcggag ggggttgaga tattcataac gttctcatgg tgcggctacc gcccgcatgg
      781 ttccgccgcc acgcgccccc ttggtaaaac caccggtgcc gtccttgggg ccggcactcg
      841 cctcatgcgc gtcagcgaac taccagatct tcgcgcgttt ttccggcgga cgccacatcg
      901 cgtcgccctc cttgatctcg aacgccgcgt agaacgaatc gagattcacc aacggaccga
      961 acgcgcgaaa atttcccggc gcatgcggac cccgcgccac ctgaagccgc atcgcgtctt
     1021 cgcgatacgc cgtccgccac tgctgcgccc acgagatgaa aaaccgctgc tccgccgtga
     1081 acccatcgat cttcggcggc acgggcttcc cttccaacga acgttgcaac gcctcgaacg
     1141 cgatcgatgt tccgccgaga tcggcgatgt tttcgccgag cgcgagctgc ccattgatcg
     1201 caagaccggg cagcgcttgg taattattga actgctcgac gacgagctgc gcgcgttcgc
     1261 gaaaacgcgt cgcgtcctcc tccgtccacc aatccaccag attgccgtcc gcatcgtagc
     1321 gtcgtccctg atcatcgaaa ccgtgcgtga tctcgtgacc gatcaccgcg ccaatcgcgc
     1381 cgtagttgac cgcgtcgtcg agtcgcgtgt cgaaaaacgg cggctgcagt atccccgcgg
     1441 gaaatacgat ctggttcgtc accggcgaga aatacgcgtt caccgtcggc ggcgtcatgc
     1501 cccattcgcg cttgtccact ttctgccccg tgcgatcgat cagccgccgc gcttcaaaat
     1561 acgccgcacg ccagacgttg ccgaaatagt cgtcgcgctt cacctcgacc gacgcgtagt
     1621 cgcgccacgt gtccggataa ccgatcatcg gctcgaaccg gtcgaacttc gccagcgcct
     1681 tctgccgcgt cggctccgtc atccattcga ctttctcgag ccgcgaacgc atcaccgcct
     1741 tgatgttgtc gatcatctcc atcattcgcg ccttcgccgc cggcgggaaa tgtttctcga
     1801 cataaagctg ccccaacgcc tcgccgatcg cgccatcgac cgccttcgtc gcgcgctgcc
     1861 agcgcggctc ctgctccggc gtgccgttca acaccgtgcc gtagaaccga aagttctcct
     1921 gctcgaatgg ctccgacaaa tacggcgcca ccgcgtgcaa caactgccag cgcgtgtaaa
     1981 ccttcagctc ctccagcggc cgctccgcca gcagtttcga caacccttcg aagaacttcg
     2041 gctgtcccac gatcacgtct ttcaccgccg ccggaatctg catttcggca acgaacagct
     2101 tgagcggaaa tccggggaac tccgccgccg cttcatcgat cgagaacttg ttgtagttcg
     2161 ccacgcggtc gcgcaggtcg accggcagct tcgcgttttc cgcgagcgtt cgctcgatcg
     2221 ccataatcgt ctccgcgtgc tgaaacgccg ccgccttcgt ctcgcccgca agctccaaca
     2281 tcttggcaat gtggccgacc agctcccaac gctcgcgcgc gaacttctcc gaaaaataat
     2341 attccttcga cggcaggctc atgccgccct ggctcaaata aaagccgtag atatcgctct
     2401 gcttctgatc cggataaaac gccgcattga agaacggcgc gccgacccgc aggtgaatct
     2461 gcgtcgccac gcgcacaagc tcgtcgacgc tcttcaccgc ctcgatcgcc gcgagctcgt
     2521 cctcgatggg cttcaaccca cgcgcattga tcgtcgccgt gtcgatcgcc gacgcgaaga
     2581 aatcgccgac cttctgccgc accgaacccg cctcgccagg cgccgccgcc gcttcttcca
     2641 cgatcgtccg cagtgccgcc cagttgcgct ccgcgagttc gttgaaaccg ccccaacgcg
     2701 acttgtccgc ggggatcttc gtccccgcat accatttccc cg
//
LOCUS       DASPLT010000667         1295 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_131356, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000667 DASPLT010000000
VERSION     DASPLT010000667.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1295)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1295)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1295
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_131356"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1295
                     /locus_tag="VEA63_06910"
     CDS             <1..>1295
                     /locus_tag="VEA63_06910"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HYD83763.1"
                     /translation="NINGNGLDNLASNADVVAGAARGNNGATSANLRGQGANATLVLL
                     NGRRIASHGLNGDVIDLNQVPFAAIERVEVLKDGASAIYGTDAIGGVINFILRTDYTG
                     LSATAATDVTEDGGGAIHRFSLLGGIGDLDRDRFNIMASLSVTEHEALRGDQRDFVNT
                     FQPDRGLSPDTRGTPIATVFAIGSLHNALSRDNLDATGRATGPTDPANPSLRVNGINL
                     VDLPNGPGYAGLDGMGPYDEVLWASPGSKYGSAWDTGRAAVLQQPVRNTHLVTRGTFE
                     VGEHRVTAEGVFGRSESTKSFSANQIASSTATTTLLPDGTSVPNPFFNLAYPSSGADY
                     ARVFDALAAYFPDIAPNRGLPLAFRWRAIPAGNREVETRSDTRRLLLSAEGPLSFLDE
                     WDYRIGLSHASSDSFSILGDGYFYSAGFASLINNGTLNP"
BASE COUNT          235 a          414 c          408 g          238 t
ORIGIN      
        1 aacatcaacg gcaatggcct ggacaatctc gcgagcaacg ccgacgtcgt cgcgggtgcg
       61 gcgcgcggga acaacggcgc gacgtcggcg aatctgcgcg gacaaggtgc caacgccacg
      121 ctcgtgctgc tcaacggccg gcgcatcgct tcgcatggct tgaacggcga tgtgatcgat
      181 ctgaaccaag tgcccttcgc cgcgatcgag cgcgtggaag tgctgaaaga cggcgcgtcg
      241 gcgatctacg gcaccgacgc gatcggcggc gtgatcaatt tcatcctgcg caccgattac
      301 accggactga gtgcgactgc cgcgaccgac gtcaccgagg acggcggcgg cgccattcac
      361 cggttttcgc tgctgggcgg aatcggcgat ctcgatcgcg accgcttcaa catcatggcg
      421 tcgttgtccg tgacggagca tgaagcacta cgcggcgacc agcgcgattt cgtcaacacg
      481 ttccagcccg atcgaggctt gtcgcccgac acgcgcggaa caccgatcgc gactgtgttc
      541 gcgatcggtt cgctgcacaa cgcgctcagc cgcgacaatc tggatgctac cggcagagcg
      601 accggaccaa ccgatccggc gaacccaagt ctgcgcgtca acggcatcaa cctggtcgat
      661 ttgccgaatg gtccgggtta cgccggcctc gacggcatgg gtccttacga cgaagtcttg
      721 tgggcgtctc caggatccaa atacgggtcg gcatgggaca cggggcgggc ggcggtgctg
      781 caacagccgg tgcgcaacac gcatctcgtc acgcgcggca cattcgaggt cggcgaacac
      841 cgggtcaccg ccgagggcgt tttcggccgc tcggagtcga cgaagagttt ctcggcgaac
      901 cagatcgcga gttcgaccgc gacgaccacg ttgctgcccg acggcacgtc ggtgccgaat
      961 ccgtttttca atctcgcgta tccgagttcc ggcgcggatt acgcgcgcgt cttcgatgcg
     1021 ctcgcggcgt atttcccgga catcgcgccc aaccgcggtt tgccgctggc atttcgctgg
     1081 cgcgcgatcc ccgcggggaa tcgagaagtc gagacgcgca gcgacacgcg tcgcttgctg
     1141 ctttcagccg aaggaccttt gtcgtttctc gacgagtggg attatcgaat cggactctcc
     1201 cacgcgagca gcgacagttt ttcgatttta ggcgacggct acttttattc cgcgggcttt
     1261 gcgtcgctga tcaacaacgg cacgctcaat ccgtt
//
LOCUS       DASPLT010000668         3672 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_193152, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000668 DASPLT010000000
VERSION     DASPLT010000668.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 3672)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3672)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3672
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_193152"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..871)
                     /locus_tag="VEA63_06915"
     CDS             complement(<1..871)
                     /locus_tag="VEA63_06915"
                     /inference="COORDINATES: protein motif:HMM:NF028461.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE sensor domain-containing protein"
                     /protein_id="HYD83764.1"
                     /translation="MAFRDLPIKRKLVAVILLTCLAVLGLTSIALLSYELFSYRQTTR
                     RTLMTIADLIAANSTAVLIYDDQKLAAEILAGLRAEPEITAAALFDKTGQLYASYSAG
                     GKTTNFPTSPGRDGLEFALQDVSVYHPVIEGANRVGTLYIHGDLSGMYSRLGIYGLML
                     LTVLGGSGFVALFLSNFFQKIISEPVLGLAETARVVSENKDYTVRAVKLSNDEIGGFT
                     EAFNSMLNQIQESHSALRTSEERLSAVFQQAGAGIAQTDLAGRFLMVNDRYCEIVGRS
                     RETLLELRMQDILH"
     gene            complement(875..1477)
                     /locus_tag="VEA63_06920"
     CDS             complement(875..1477)
                     /locus_tag="VEA63_06920"
                     /inference="COORDINATES: protein motif:HMM:NF025069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiR family protein"
                     /protein_id="HYD83765.1"
                     /translation="MSNAHAFSVRCATFAPCIGWRGILSFVAVLCALLAPLSAAELTK
                     ESDVKAALLYNFTQFAEWPPSAFEGADDPFVIGIMGRDPLGGVLDKLVEHEAVAGHRI
                     VVTRYRNVEAARQAHVLYISASERAELSHILPALAGHPVLTVADFDGFLDRGGMVVLQ
                     RGRNQKIRIRVNLAAAQAASLTFSAKLLRVVEIVSPEEEP"
     gene            complement(1597..3312)
                     /locus_tag="VEA63_06925"
     CDS             complement(1597..3312)
                     /locus_tag="VEA63_06925"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HYD83766.1"
                     /translation="MKKMSLEELLSIHVTTMSRKEELWWAAPGAIEVLTSEEIRRSGA
                     QNLPDALRLATGIDVAQPSARSWAVSARGFNVLAANKISVLMDGRSLFTPFFSGVQWD
                     AQDTLLADIDRIEVVRGPVGALWGSFAVNGFIQIVTKSARDTQGTLVSAGAGTEDPGF
                     VAMRHGGRIGRQTFYRAYAKYYKTEWSYLANGQHSQPPTDFFQTGFRIDSKLDADTSA
                     TLQGDVYTNKGTPEDHPQGETSGGNVLARVTRSLAAGSDVEILSYFDHTRRFIPQNWL
                     ETRNTGALTLKYRRPTGAHDLLFGFDANVSRDDIVELGVATMDPPQRTTHTASVYVQD
                     TWAISPGRTALTFGGKLEHNAFSGFEYHPSLRGAWTPTADTTWWAAVSRAVRSPVRVD
                     HDLRFDFNGVTIVRASDEFRSETAIAYEIGWRRRFGETATIDVATFYNEYDHLRTTEP
                     EGAAALPVTFKNGLRARSLGVETTLMYQPFTRLFFKLSHRLLDLDFLETPGTRDTTHG
                     NTEGNDPRHLVTLSAHAGLPWDLEFDATLRHVGKRPNPASDAYTVADLRLGWSPTDRW
                     DFSLI"
BASE COUNT          699 a         1224 c         1102 g          647 t
ORIGIN      
        1 cgtgcaggat gtcctgcatg cgcagctcca ggagcgtttc gcgcgagcgg ccgacgattt
       61 cgcaataccg atcgttgacc atcaggaaac gccccgcgag gtccgtctgc gcgatgccgg
      121 cgcccgcctg ctggaaaacc gcggagagcc gctcctcgct cgtgcggagc gccgaatggc
      181 tttcctgaat ctggttcagc atcgagttga acgcctcggt gaaaccgccg atctcgtcgt
      241 tgctcagctt cacggcgcgc acggtgtaat ccttgttttc cgacacgacg cgcgcggtct
      301 cggcaaggcc gagcaccggc tccgaaataa ttttctggaa aaagttcgag aggaacagcg
      361 ccacaaatcc cgagccgccg agcacggtca ggagcatcaa accatagatg ccgagccgac
      421 tatacattcc gctgagatcg ccgtgaatgt agagcgtccc cacgcgattt gcgccctcga
      481 tcaccggatg ataaacgctg acatcctgca gcgcgaactc gagaccgtca cgccccggcg
      541 aagtcggaaa gttcgtcgtc ttgccgccgg ccgaatacga cgcgtaaagc tgccccgtct
      601 tgtcgaagag cgccgccgcc gtgatctcag gctcggcgcg caggcccgcg agaatctccg
      661 cggcgagttt ctggtcgtcg taaatcagca ccgccgtgct gttcgctgcg atgagatcgg
      721 cgatcgtcat cagcgtgcgg cgggtcgtct gccggtagct gaacaactcg tagctcaaca
      781 gcgcgatcga cgtcagcccg agcaccgcca ggcaggtgag caggatcacg gcgacgagct
      841 tgcgtttgat gggaaggtcg cggaaggcca tcgcctaggg ttcctcttcg ggcgaaacga
      901 tctcgaccac gcgaagtaat ttcgcgctga aggtcagcga ggcggcttga gcggcggcca
      961 ggttcacgcg aatccggatt ttctgattcc ggccgcgctg cagcacaacc atgccgcctc
     1021 ggtccaggaa gccatcgaag tccgccaccg tgaggaccgg gtgtcccgcg agcgcaggaa
     1081 gaatgtgaga aagctccgct cgttcgctgg cgctgatgta gagcacatgg gcctggcgcg
     1141 cagcttcgac atttcgatac cgcgtgacca caatccgatg cccggcaacc gcctcatgtt
     1201 ccacgagttt gtcgagaact ccacccagcg ggtcgcgccc catgattccg atcacgaacg
     1261 gatcgtccgc accttcgaac gccgacggcg gccactcggc aaactgcgtg aagttataga
     1321 gcagcgccgc tttcacgtcc gactctttcg tcaactccgc tgccgacaac ggtgcaagca
     1381 gcgcgcagag caccgccacg aagctcaaaa tgccgcgcca gccgatgcac ggcgcgaacg
     1441 tcgcgcagcg aacggagaaa gcgtgggcgt tcgacatcgt ggacatgctc gcggtcagta
     1501 tctccacgtt gctttcatcg tgccactcgg gccgacaaat tcgttcaaac tattcgtggt
     1561 aacgagctcc cggtgcaggc cgttgaatag gtttcgtcag atcagcgaaa aatcccatcg
     1621 atcggtcggc gaccagccga gccgcaagtc ggccaccgta tacgcatcgc tcgccggatt
     1681 tggccgcttg ccgacgtggc gcagggtcgc atcgaattcc aaatcccacg gcaagcccgc
     1741 gtgcgcgctc aacgtcacaa ggtgccgcgg gtcgttaccc tcggtgttgc cgtgagtggt
     1801 atcgcgcgtg ccgggcgttt ccaaaaaatc caggtcgagc aggcggtgac tgagtttgaa
     1861 aaacaatcgc gtgaacggct gatacatcag cgtcgtctcc acgcccagcg accgcgcgcg
     1921 caggccgttc ttaaacgtca ccggcaacgc cgcggcgccc tccggctccg tcgtgcgcag
     1981 gtgatcgtat tcgttgtaga acgtcgccac gtcgatcgtc gccgtctcgc cgaaccgccg
     2041 ccgccagccg atctcgtagg caatcgccgt ctccgagcga aactcatcgc tcgcgcgcac
     2101 gatcgtgacg ccgttgaagt caaagcgcag atcgtggtca acgcgcaccg gcgaacgcac
     2161 ggcgcgcgac accgccgccc accacgtcgt gtcggcagtc ggcgtccacg cgccgcgcaa
     2221 cgacggatga tactcgaaac cgctgaacgc gttgtgctcg agtttgccgc cgaacgtcag
     2281 cgccgtgcgc cccggcgaga tcgcccacgt gtcctgcaca taaacggagg ccgtgtgcgt
     2341 cgtgcgctgc ggcgggtcca tcgtcgccac gcccaattcc acgatgtcgt cgcgcgaaac
     2401 gttggcgtcg aagccgaaga gcaggtcgtg cgcgccggtc ggacggcggt atttcaacgt
     2461 gagcgcgccg gtgttgcgcg tctcgagcca gttttgcgga atgaaccgcc gcgtgtgatc
     2521 gaagtaggaa aggatctcca catccgagcc ggccgcgagt gatcgcgtga cccgcgcgag
     2581 cacattccca cccgaggttt ctccctgcgg atgatcctcc ggcgtgccct tgttcgtgta
     2641 gacatcgccc tgcagagtcg cggaggtgtc ggcatcgagc tttgaatcga tgcggaaacc
     2701 cgtctggaaa aaatccgtcg gaggctgcga atgctggccg ttggcgaggt aactccactc
     2761 cgttttgtaa tacttcgcgt aagcgcgata aaacgtctgc cgaccaatcc gcccgccgtg
     2821 ccgcatcgcc acgaagcccg gatcctcggt gcccgcgccc gcgctcacca gcgtgccctg
     2881 cgtgtcgcgc gcggatttcg tcacgatctg gataaaaccg ttcaccgcga acgatcccca
     2941 gagtgcgccc accggcccgc gcaccacttc gatgcgatcg atgtcggcga gcagcgtgtc
     3001 ctgcgcgtcc cattgcacgc cggagaaaaa cggcgtgaac aaactccgcc cgtccatcag
     3061 cacggaaatc ttgttcgccg cgaggacgtt gaaaccccgg gcgctcaccg cccagctccg
     3121 cgcactcggc tgcgccacgt cgatgcccgt cgcgagccgc agcgcatccg ggagattctg
     3181 cgcgccggag cggcggatct cctcgctggt caacacctcg atcgcgcccg gcgccgccca
     3241 ccagagctct tctttgcgcg acatcgtcgt cacgtggatg ctcagcaatt cctcgagcga
     3301 catttttttc atgctctcga cgctggcgac gagcggctgc gcccgaccgc tcggcacccc
     3361 agctcccttt gccagcaaag ctgccaccaa aacgccgcac cacgacacgc cgccacgacg
     3421 gagtctcggg agcctacatc caggagagaa ttgggtcatg agctggagaa acgaagagcc
     3481 tgaacagctt gcagggaagt gaaccgcgcc gtcgagtgcc gtatcgcgcg gcattcagcc
     3541 ggccgatttg gccgcgccca tcgtggcgag ggctttttcg agtgcttgca tgcgaatcgg
     3601 cttcgtcagg tgcacgacaa atcccgcccc ttcgctgcgc gcgacgtcgt cttccatgcc
     3661 atagccgctc ag
//
LOCUS       DASPLT010000669         2286 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_131386, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000669 DASPLT010000000
VERSION     DASPLT010000669.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 2286)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2286)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2286
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_131386"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            257..1270
                     /locus_tag="VEA63_06930"
     CDS             257..1270
                     /locus_tag="VEA63_06930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HYD83767.1"
                     /translation="MKAIFDARTLSHQDRAEMRRIFDLWIKQADRGLLRMRIPKEVKP
                     TQNLRGMHYHYRPEIFLQVSGRTEFRFPRERFDLLPGEICIVPAGVPHGEIVHPDAGQ
                     PFRNLVAGFYNNTISLHFAYEAGPQRPDIEAIEFFDAPNLDVFLTLTNSLAHAFSMQA
                     PARDAVLKGLLVALLGLFRNIIETGAGNLNGDVGKVFQAKCLVREQFSNPELRVQDIA
                     AMLGCSADYLSHLFHVETEERLTHYIQRIRIEGAILALGTTALTIAEIAYTSGFADPA
                     YFARVFKQHKAMTPQEFRAQLDAHRNRPEPQPKTVYANRVDFTHGTPRKPLAPSAEAL
                     AEA"
     gene            complement(1280..>2286)
                     /locus_tag="VEA63_06935"
     CDS             complement(1280..>2286)
                     /locus_tag="VEA63_06935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HYD83768.1"
                     /translation="HYRAAEFWLKEIGVDGFRLDAVRYFYEIGEALQDTEETRQWLKA
                     FTEHCHAVNPAAFVIGENTARMNEVARSIRGDSVDSSFEFDLARAVLESVRLRTPAIL
                     SNALARLDETYEGQTPWATLLTNHDQERARTQLGGSDALTRLATKLLFTLPGVPFLYY
                     GEELGMQATKPDPELRTPMPWTAEPPNAGFTAREAQAWKAPQPEFVDVNVAAQTSDPD
                     SMLTLYRRLIRLNASSPALRYGKAIEVTTNHRAVYAQMRQTDREVVLVLGNFGETPLH
                     DVHLTVEESVASDTWTLSEALEGEEIAKPALTERGGFQQWRPLAELAPESIYVLRWRR
                     "
BASE COUNT          434 a          800 c          598 g          454 t
ORIGIN      
        1 agcgtcgtgt agcggtgcaa ttggtaggag cctgcctgca ggctcctacc tccgattcgt
       61 cccgggaaaa attccggttc aactctgaac ctccgtgcgc ccacgaacgc gcatcggaaa
      121 aatcctgaaa agaccggata aaacctattc gcgttcgcgc gggcgtcgct tttctgcaca
      181 ctgctgcaag tccgcggcgt tcggcgcgcg gctccctctt ctgctacccc gttcctcacg
      241 cgttttcgcg ttccccatga aagctatttt cgatgccagg accctgagcc atcaggaccg
      301 cgccgagatg cgccgcattt tcgacctctg gatcaagcaa gcagaccgcg gtcttttgcg
      361 gatgcgcatt ccgaaggaag tgaaacccac gcagaacttg cgcggcatgc actatcacta
      421 tcgtcctgaa atttttctcc aggtgagcgg ccgcaccgaa tttcgatttc cgcgcgaacg
      481 tttcgatctc ctgcctggcg aaatctgcat cgtccctgcg ggcgtgcctc acggtgaaat
      541 cgtgcacccc gacgctggcc aaccgttccg taaccttgtc gccggtttct acaacaacac
      601 gatcagcctt cacttcgcat acgaggccgg tccgcagcgg cctgacatcg aagcgatcga
      661 gtttttcgat gcgccgaacc tcgacgtttt tctcacgctg accaacagcc tcgctcacgc
      721 gttttcaatg caggcgcccg cgcgcgatgc tgtgctgaag ggtcttctcg tcgcgcttct
      781 cggcttgttt cgcaacatca tcgaaaccgg cgccggcaac ctcaacggcg acgtcggcaa
      841 agttttccaa gcgaaatgcc tcgttcgcga gcagttctcc aatcccgaac tccgcgtgca
      901 ggatatcgcg gcgatgctcg gctgctcggc cgactatctc tcacacctgt tccacgtcga
      961 aactgaggag cgtctcacgc actatattca gcgaatccgc atcgaaggcg cgattctcgc
     1021 gcttggcacc accgcgctca ccatcgctga aatcgcctac accagcggct tcgcggaccc
     1081 cgcgtatttc gcacgtgtgt tcaagcagca caaagcgatg acgccgcagg aatttcgcgc
     1141 ccagctcgac gcgcatcgca accgccccga gcctcagccg aaaaccgtct acgccaaccg
     1201 cgtcgatttc acgcacggca cgccgcgaaa accgctcgcg ccctccgccg aagcgctcgc
     1261 ggaagcgtga gcgtttatct caccgccgcc accgcagcac ataaatcgat tccggcgcca
     1321 actccgccaa cggtcgccat tgctgaaatc caccgcgctc ggttagcgcc ggcttggcga
     1381 tctcctcgcc ttccaacgct tcgctgagcg tccacgtgtc ggatgccacg ctttcctcga
     1441 ccgtgagatg aacatcgtgc aacggcgttt caccgaaatt gcccaacacc agcacgacct
     1501 cgcggtctgt ttggcgcatc tgcgcgtaaa ccgcacggtg gttcgtcgtc acctcgatcg
     1561 cctttccgta gcgcagcgct ggcgagctcg cgttcaaccg gattagccgg cgataaagcg
     1621 ttagcatcga gtccggatcg ctcgtttgag ccgcgacatt cacgtcgacg aactccggct
     1681 gcggcgcctt ccacgcttgc gcctcgcgcg cggtgaaccc cgcgttcggc ggctccgcgg
     1741 tccaaggcat cggtgtgcgc agctccggat ctggcttcgt cgcctgcatc cccaattcct
     1801 cgccgtagta gagaaacggc acgcccggga gcgtgaacaa aagcttcgtc gcgagccgcg
     1861 tcagcgcgtc gctgccgccg agctgcgtgc gggcgcgctc ctgatcgtga ttcgtgagca
     1921 gcgtcgccca cggcgtttgc ccctcgtaag tctcgtccaa tcgcgcgagc gcgttcgaaa
     1981 gaatcgccgg cgttcgcaac cgcaccgact ccaatacagc gcgcgcaaga tcgaactcga
     2041 aggagctgtc caccgaatcg cctcgaatcg accgagccac ctcgttcatc cgcgcggtgt
     2101 tctccccgat aacgaacgca gcggggttca ccgcatgaca atgctccgtg aacgccttca
     2161 accactgccg cgtctcctcc gtgtcctgca acgcttcgcc gatctcgtaa aagtagcgca
     2221 ccgcatcgag ccggaaccca tcgacgccaa tttccttcaa ccaaaactcc gccgctcgat
     2281 aatgat
//
LOCUS       DASPLT010000670         1606 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_295061, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000670 DASPLT010000000
VERSION     DASPLT010000670.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1606)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1606)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1606
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_295061"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            45..>1606
                     /locus_tag="VEA63_06940"
     CDS             45..>1606
                     /locus_tag="VEA63_06940"
                     /inference="COORDINATES: protein motif:HMM:NF013239.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIM-barrel domain-containing protein"
                     /protein_id="HYD83769.1"
                     /translation="MADPGQPGAKIDTLKGEHFVQMVEKLQALSIPVGVTWFEPPWQD
                     ARNSFVPNPAFSADLRGLVRQLEERGVRTLAWTVPYTTAEASNWKEAVGRGYLVRKPG
                     TTVDGAKVKVTASGELEGRYYNMIDLFNPEAFAWWRAQIERSLETGIRGYKLDAGQDL
                     PADALLHGGRRGADVHNSYAAEYSRVFFEALQKKYGDDFLMVPRAAWMGSSAFTNFKW
                     PGDLSGSYANNGLPSSVYSSLSLAFSGFPFVSTDIGGFEDQPAPEHVWLRWAQFGAML
                     PGMQTLHMRWWYSEESLAHFRFLTWLHTDMTPLWMSLAHEAAATAAPICRPLVWSDQA
                     DVETWRVDDQFTVGESLLVAPLLNNNYDRAVYLPEGRWYDFWDNRETFVGPAHVTWFK
                     GWFVKDKFPLYIKEGAIIPLEVINEFSGFGWPESSGFVTLAMWPRVRGVSEFTLQDTE
                     APVRITAEWGGDDVLTLGWSETRRNHLLRVHLDSSFGVGSVADSTGLLNRFDSLDAFR
                     GGDEGWFHDRDTG"
BASE COUNT          277 a          451 c          547 g          331 t
ORIGIN      
        1 ctcggcagcc gccgcggtgg acgtttaagc cgtggttctg gctcatggcc gatcccgggc
       61 agccgggcgc gaagatcgat acgctcaagg gcgagcattt cgtgcaaatg gtcgagaagc
      121 tgcaggcact ctcgattccg gtgggcgtta cgtggttcga accgccgtgg caggatgccc
      181 gcaattcctt cgtgccgaat cccgcgttca gcgcggatct gcgcgggctg gtgcggcaat
      241 tggaagagcg cggcgtccgc acgctcgcgt ggacggtgcc ttacacgacg gcagaggcgt
      301 cgaattggaa ggaagcggtt gggcgcggct atctcgtgcg gaaacccggc accacggtcg
      361 acggggcgaa ggtgaaggtg acggcgtcag gcgaactcga aggccggtat tacaacatga
      421 tcgatctgtt caatccggag gcgttcgcgt ggtggcgcgc gcagatcgag cgaagcctcg
      481 agaccggaat ccgcggttac aaactcgacg ccggtcagga cctgccggcc gacgcgttgc
      541 tgcacggcgg gcgtcgcggg gcggacgtgc acaattctta cgcggccgaa tacagccgcg
      601 tgtttttcga ggcgctgcaa aaaaaatacg gcgacgattt tttgatggtt ccgcgagccg
      661 cgtggatggg ctcctcggcg ttcaccaatt tcaagtggcc gggggacctg agcggaagtt
      721 atgcgaacaa cggcctgccg tcgtcggtct actcttcgct ttcgctggcg ttcagcggat
      781 ttccctttgt ttcaacggac atcgggggct ttgaagacca gccggcgccc gagcacgtgt
      841 ggctgcgttg ggcgcagttc ggcgcgatgc tcccgggcat gcaaacgctg cacatgcggt
      901 ggtggtattc ggaggagtcg ctcgcgcact ttcgtttttt gacgtggctt cacactgaca
      961 tgacgccgct ttggatgagc ctcgcccatg aagccgccgc gaccgccgct ccgatttgtc
     1021 gcccgctggt ctggtcggat caggccgacg tcgaaacctg gcgcgtagac gaccagttca
     1081 ccgtcggcga atcgctgctc gtcgcgccgt tgctcaacaa caactacgat cgcgccgtct
     1141 acctgccgga aggacgctgg tatgattttt gggacaatcg ggaaacgttt gttggccctg
     1201 ctcacgtcac gtggttcaag ggctggttcg tgaaagacaa atttccgctc tacatcaagg
     1261 aaggcgcgat catcccgctg gaggttataa atgaattcag cggcttcggc tggccggagt
     1321 cgtcgggttt cgtgacgttg gcgatgtggc cgagggttcg cggtgtgagc gagtttacgc
     1381 tgcaagacac ggaagctccg gtgcgcatca cggccgagtg gggcggggac gatgtgctga
     1441 cgcttggctg gagcgaaacg cgtcgcaatc acctgctgcg cgtgcacctc gattcatcgt
     1501 tcggcgtcgg cagcgtcgct gattccacgg gtttgctgaa ccgtttcgat tcgttggatg
     1561 cgtttcgggg cggcgacgag ggctggtttc acgatcgcga caccgg
//
LOCUS       DASPLT010000671         2148 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_68267, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000671 DASPLT010000000
VERSION     DASPLT010000671.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 2148)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2148)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2148
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_68267"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1490)
                     /locus_tag="VEA63_06945"
     CDS             complement(<1..1490)
                     /locus_tag="VEA63_06945"
                     /inference="COORDINATES: protein motif:HMM:NF014763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HYD83770.1"
                     /translation="MSLSRFLPKTIGARLVVAIGLASALVIGSISLTAFRTGRTSLIA
                     QASADAFTEVNTAAAAVDEAINRLAYVPRMLAAYQAGVGREPEPHLEYIIRNLLRRIP
                     AREIYGTYIAFDAKEWSDPKGMIWMDRVSWPALRRIDYDYHDPKQDWFAGAKRTQQLH
                     LTEPFFDEDGSNITMVSITFPVLIDRQFIGAAGVDLPLTEIGALIESIHERAANRRAR
                     DSGEFIFLASREGRLIAHPDASHLPREGYAGTTLADLPEGERLARDTEGSATYTAANG
                     EERILSWTVAPTTGWKVVLDQPASLVAAPVGALARRTALIGYGGLVVLLALVGLIARR
                     ATRPIVKLERAAKALETGAFNAADLDPLVARVDETGRLAGAFQSMAREINAREERLAE
                     WNQNLELTIEQRTADLRLAREAAEEASRTKSAFLANMSHELRTPMNAIIGYSEMLVEE
                     AEDTGQDAFVPDLKKIHSAGKHLLGLINDVLDLSKIEAGKMTLYLE"
     gene            complement(1605..>2148)
                     /locus_tag="VEA63_06950"
     CDS             complement(1605..>2148)
                     /locus_tag="VEA63_06950"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007362364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HYD83771.1"
                     /translation="GAALLSVTAINVLRNAALPSVDFRDPGAMLTALNEAFLMEKQND
                     MYFTIWYGVWQPSTRTLRFATAGHPPALLVTPQPDGSGAVELLKGAGMILGGMSGASY
                     RTFERRLTGPARIYIVSDGTFEIARPDGTMWDFAEFQASFAAAPRENESDLDRLFAHV
                     KSLHGPGPLDDDFSIVRFEL"
BASE COUNT          352 a          547 c          848 g          401 t
ORIGIN      
        1 tcctcgaggt agagcgtcat cttgccggct tcgattttgg agagatcgag gacgtcgttg
       61 atgaggccga gcaggtgttt gccggcggag tggatttttt tgaggtcggg gacgaaggcg
      121 tcctggccgg tgtcctcggc ttcttcgacg agcatttcgc tgtagccgat gatggcgttc
      181 atcggggtgc ggagctcgtg gctcatgttg gcgagaaatg cgctcttggt gcggctggct
      241 tcctcggcgg cttcgcgggc gaggcggagg tcggcggtgc gctgctcgat ggtgagctcg
      301 agattttgat tccactcggc gaggcgttct tcgcgggcgt tgatttcgcg ggccatgctt
      361 tggaacgcgc cggcgaggcg gccggtttcg tcgacgcggg cgacgagcgg gtcgaggtcg
      421 gcggcgttga acgcgccggt ctcgagggct tttgcggcgc gttcaagttt gacgatgggg
      481 cgcgtggcac ggcgggcgat caagccgacg agcgcgagca ggacgacgag gccgccgtag
      541 ccgatgagcg cggtgcggcg ggcgagcgcg ccgacgggtg cggcgacgag cgaggcgggt
      601 tggtcgagga cgactttcca gccggtggtg ggtgcgacgg tccaggagag gatgcgttct
      661 tcgccgttgg cggcggtgta ggtggcggag ccttcggtgt cgcgcgcgag acgttcgccc
      721 tccggcagat cggcgagcgt ggtgccggcg taaccctcgc ggggaagatg cgacgcgtcg
      781 ggatgcgcga tcaggcgacc ttcgcggctg gcgagaaaaa tgaattcgcc gctgtcgcgg
      841 gcgcggcggt tggcggcgcg ttcgtggatg ctttcgatga gcgcgccgat ttcggtgaga
      901 ggcagatcga cgccggcggc gccgatgaac tggcggtcga tgagcacggg aaacgtgatg
      961 ctcaccatgg tgatgttgga gccgtcctcg tcgaaaaacg gttccgtgag atgaagctgt
     1021 tgcgtgcgtt tcgcgccggc gaaccaatct tgtttcgggt cgtggtaatc gtagtcgatg
     1081 cgacgcagcg cgggccagct cacgcggtcc atccagatca tgcctttcgg atcggaccat
     1141 tccttggcgt cgaaggcgat gtaggtgccg tagatttcgc gagcgggaat gcggcggaga
     1201 agattgcgga tgatgtattc gaggtgcggc tcgggttcgc gaccgacgcc ggcctgatag
     1261 gcggcgagca tgcgcggaac gtaggcgagg cggttgatgg cttcgtcgac ggcggcggcg
     1321 gcggtgttga cctcggtgaa cgcgtcggcg ctggcttgcg caatgagcga ggtgcggccg
     1381 gtgcgaaacg cggtcagcga gatgctgcca attaccagtg cggaggcgag gccgatggcg
     1441 acgacgagcc gggcgccaat ggttttgggg aggaagcggg agagggacat cggacaaaaa
     1501 gtgaagaatg aattggccgc aaagaggcgc agaaggcgca agaaaagctg agggccgggg
     1561 gatttttttt cgccttttgc gcctctttgc ggctaaagtt tgggctagag ttcgaagcgg
     1621 acgatcgaga aatcgtcgtc gagcgggccg gggccgtgga gggatttcac gtgagcaaaa
     1681 aggcggtcga ggtcggactc gttttcgcgc ggcgcggcgg cgaacgaggc ttggaattcg
     1741 gcgaagtccc acatcgtgcc gtcgggacgc gcgatctcga atgtgccgtc gcttacgatg
     1801 tagattcgtg ccgggccggt caggcggcgc tcgaaagtgc ggtagctggc gccgctcata
     1861 ccgccgagaa tcatgccggc gcctttgagc agttcgacgg cgccggagcc gtcgggctgc
     1921 ggcgtgacga gcagcgcggg cggatggccc gcggtggcga agcgcaacgt gcgcgtcgac
     1981 ggctgccaca cgccatacca gatggtgaaa tacatgtcgt tctgcttctc catcaggaac
     2041 gcttcgttca aggcggtgag catcgcgccg ggatcgcgaa aatcgacgga gggtagggca
     2101 gcgttgcgga ggacgttgat ggcggtgacg gagagcaacg cggcgccg
//
LOCUS       DASPLT010000672        16633 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_213209, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000672 DASPLT010000000
VERSION     DASPLT010000672.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 16633)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16633)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16633
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_213209"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            104..328
                     /locus_tag="VEA63_06955"
     CDS             104..328
                     /locus_tag="VEA63_06955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD83772.1"
                     /translation="MDEAAVELARTYAASIDANPLMVGQLGGGLKAMLQELGMTPKAR
                     ADLMGGKVAPAANPSLDELRARREQRARQR"
     gene            1140..2345
                     /locus_tag="VEA63_06960"
     CDS             1140..2345
                     /locus_tag="VEA63_06960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD83773.1"
                     /translation="MILTLYWLYVDRVRLVLGTSTNLDTARESWEKAVSLAEDNPTLA
                     SEVGDVRRTNGEQTLTTVWGGRYKIAASNRKGGRGLTIDRLVLDELREHAHWDSYNAA
                     VPAMNAVPDATAFFISNQGDDKSQVLNALRDQALEGSDERLGLFEWSSEDGMDPTDPE
                     ALAMANPSLGRRIFLDTLMGDAIRAKQAGGEQLADFLTEVHCRRVPLLNPAIDLDAWS
                     RSYSAENSLEAFKDKLALCVDVSFDQTHATLYAAAVDGERVRVDPVAAWEGQDAISQM
                     SSQLPALVEKMKPRVLGWFPDGPAAAALPALTPRQGWPPAGVTVEAIRQVTAVCMGFA
                     EQVRSDKVYHSGDPLLDAHVRAAEKLSYGDGWRFTRRKAGHVDAAYAAAGAVHLARTL
                     QDESEPMIF"
     gene            2643..3767
                     /locus_tag="VEA63_06965"
     CDS             2643..3767
                     /locus_tag="VEA63_06965"
                     /inference="COORDINATES: protein motif:HMM:NF016732.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HYD83774.1"
                     /translation="MSVPAFGRAGNIIAGTIAGFPLHIYESGQDGQRRLAQDDSNRFL
                     WGVGGPRSKNNDRPLGPNPEVPAFVFWKVVATHKFWRENVYLFVVSDNARRTLELWPI
                     DPVRVRVGRLADGRKIFEVDGKEAYLDYLHGGEIVHVVGDSEDGLVGVGPLQRYADTF
                     ETAISTEQYASRQFSNNTNPSGILKTDQQLTPDRAEELLARWEARHAGGVNAGRPAVL
                     SNGLEWQTIQFNPEETQMLESRKFGVFLMEILSGVPPHLLGDVERSTSWGSGIEEQSR
                     NFLTYTLSNHYIPIEQTITMAFLPKDRFALFNPDAILRPDTFKRAQVHEIMIRSRMAT
                     PNERRAYENMEPIEGGDELAAPPNASLPAPTERRSTEEGE"
     gene            3767..4024
                     /locus_tag="VEA63_06970"
     CDS             3767..4024
                     /locus_tag="VEA63_06970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD83775.1"
                     /translation="MGEQITEQMWGLHADREAPCDRCYAMISMVIAGQSTWQNINDLH
                     HDCRLAAINVGLIRWVPVQRMSLSDAGQLRTLIEAFEEGNE"
     gene            4021..4170
                     /locus_tag="VEA63_06975"
     CDS             4021..4170
                     /locus_tag="VEA63_06975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD83776.1"
                     /translation="MMGVQTIPAAIEQKIEAFCGQKKSGRFILDISEGRIVGWEILEK
                     GRIRG"
     gene            4675..5574
                     /locus_tag="VEA63_06980"
     CDS             4675..5574
                     /locus_tag="VEA63_06980"
                     /inference="COORDINATES: protein motif:HMM:NF013506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HYD83777.1"
                     /translation="MADEAVRAIVLDIDSPGGTVAGAPELAAEIYRARGRKPVVALAN
                     AHAMSAAYWLGSSAEEFYITPSGEGGSIGVIASHMNLSGAAEKAGIQPTILSAGKYKA
                     EVNEFEPLNDEALAAAQHRIEAAYDLFVHDVARNRGATEAQVRNGYGEGRYLVAKDAV
                     KAGLFDGERTFEQVLRNIGQSRSSSPAAALEETERAAESIPFGVHATQALAAMQAFIS
                     RAQALAALRAEEGRPLGESARNALFSFAEAVAEQTQLVDQLIRNQPDARARQDAEAFA
                     RFRVALLRDEAAATQQALEMNAR"
     gene            5571..6881
                     /locus_tag="VEA63_06985"
     CDS             5571..6881
                     /locus_tag="VEA63_06985"
                     /inference="COORDINATES: protein motif:HMM:TIGR01554.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HYD83778.1"
                     /translation="MTTQTRADQARERLVEATAVLIEELAKYENTPPTPEQLEHLNEL
                     KASKDKAKADFDEVGKFEALRVDAATMQDFLKQPANRPPHADSGEPMRERRGRISPKS
                     LGQKFAADENYQAWLKSIAPNGKIPDSARIPSSPAFAFGGLNDLQPMAALLTGASSTS
                     GGAMIYDDNYPGLIGLGRRELTIRDLVTPGQTDSDTVTYVRVTSETNNAAPVAEATAT
                     GDGSGVKPESAMAFERVSTTVKTIAHWIPATKAALADAGQLRTLIDAFLRDGLEEELE
                     DQMLTGDGSGENFTGIFNTTGTQTQAWDTDIFATTRKAITKMRTVARRRPTAWAMLPA
                     TLEFLDLQKDGEERYYNGGPFGPAGRTLWGYPIVETEGLPTGFALLGDFKRAVLWDRE
                     QASIQVSDSHSDFFIRNLVAILAEMRAAFGVLQPNAFVEVDVVA"
     gene            6915..7277
                     /locus_tag="VEA63_06990"
     CDS             6915..7277
                     /locus_tag="VEA63_06990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD83779.1"
                     /translation="MSDTLNGAGLDRVSVKTANYTVVAATDNGKVFTNEGAAGAITFA
                     LPAATVGQRYGFHVMAAQELRIDPNGTETISLPTGVQQAAGKYIGADAAGEYILIRCV
                     KAGQWNAFGAVGTWTAEA"
     gene            7445..7687
                     /locus_tag="VEA63_06995"
     CDS             7445..7687
                     /locus_tag="VEA63_06995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD83780.1"
                     /translation="MNTADGKTTINVRNEELEKYLAANPGAEVVQPERTATRSRTRAQ
                     AEDASTEDADGDQDEQETDGDDGPRRSPRARTTRRS"
     gene            7709..8353
                     /locus_tag="VEA63_07000"
     CDS             7709..8353
                     /locus_tag="VEA63_07000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD83781.1"
                     /translation="MELLDPYVSVDEYRTRAGSLAIADDPTLAAQLVAVSRLIEEVLK
                     VAPGAFNSVEGTRYFDSDGTATLRLRDAQGKQHFLQSVDANAIGVDNSSSGAFTDYAL
                     DFDDDWVVGVGGLGQPYEAITISPITSAAPYYWPRGVRTVSITGVWGWPSVPGVIKEL
                     VVGLARDMRQGQLTGSGGSIADFEGFNGRALSPGTYFWWTEAKRLLSRELTAFA"
     gene            8350..8808
                     /locus_tag="VEA63_07005"
     CDS             8350..8808
                     /locus_tag="VEA63_07005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD83782.1"
                     /translation="MSWLTARAALAALLNGQALQVEGYREETLTCVSTPKAMPDAPGF
                     PLAVVRRPASRVVRRSPGGLRNLEVTAEVDVMVAAETLDEAEERLEAWVQHLMDLVGA
                     HLKFDGQTDVTVTGQEFGEFGSYSDQANASPFGFPMQLTVLLTDTETRGA"
     gene            8819..9082
                     /locus_tag="VEA63_07010"
     CDS             8819..9082
                     /locus_tag="VEA63_07010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD83783.1"
                     /translation="MAWKFKGEGLPLPGIGAGPFTDEEFEIAVSVYEAQFSDDQKGAV
                     KASGLYERTSLEKVADELPDTPPPVVAEAKADEQPRRRGGRTE"
     gene            9083..10081
                     /locus_tag="VEA63_07015"
     CDS             9083..10081
                     /locus_tag="VEA63_07015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD83784.1"
                     /translation="MAITASGLKVYQGGPEVTPGTPVAATRKFSLENLDWTPQDEVYR
                     PRFANGVLLDNRGGETVISRGSEFTGEGPVIPDELHRWASMCIAADVITGSGPYTHTF
                     TRNPVANPTLKTWTLERRLSDGSNAIDNEWSHALLRSLTISAAMDAPLRLSFAGIARR
                     VLDSTLTAALSQVTTEILPSSLSTVFIDPAWADLGETQVTQQVLSFSVTFRSGINPVK
                     TADGRTDLDFGIVTVNDEEVGIDISMRLMVKADSGQYATEKTAAEAQSLRAVRIAVAG
                     AGDTEITIDALCKHVAGSLFKVDEQDGYEVFDMNLVSSTDLTNAYRLVVINAASAL"
     gene            10092..10439
                     /locus_tag="VEA63_07020"
     CDS             10092..10439
                     /locus_tag="VEA63_07020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD83785.1"
                     /translation="MAEKHRIKLDDLLGEDYAGEWVDIHKARTWGARLKVQDAARGDV
                     SDYKLTVLDVSIVGWSRSEPHERSAYEALAEEAGEALFEAITGYYASRRRAADASKSA
                     VAAGADEPGERAA"
     gene            10625..11014
                     /locus_tag="VEA63_07025"
     CDS             10625..11014
                     /locus_tag="VEA63_07025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD83786.1"
                     /translation="MRGALRNLGPEFVKELQRENKAVANEIGQEAGRAFAAAHPGGSG
                     RSAATYRGLATQTSAKVAAGSARRLHVLGFDFGSNQGKNKKQFPPYVKGGRFLYPTIQ
                     RRSADLPERYGKVLDKVGRPVFPGGSR"
     gene            complement(11016..11267)
                     /locus_tag="VEA63_07030"
     CDS             complement(11016..11267)
                     /locus_tag="VEA63_07030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD83787.1"
                     /translation="MQSAQTLTPAPSKAIPALALLLGVGLFIVWSMGAFDVVLMKLGW
                     ESLVSQPCIRLITDAVICGREEIDAMCAAWSNTANFCTP"
     gene            11363..13255
                     /locus_tag="VEA63_07035"
     CDS             11363..13255
                     /locus_tag="VEA63_07035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD83788.1"
                     /translation="MKQMDGELGTVETTTTRVGRTMQNAFAIGATAIAAGTAAAAAGA
                     GAVLNLANSYAEAGSQIFDLSQRTGFSTEALSEWKYAADQSGASIEGLEVGIRRMSST
                     VVDAGNGLDSAKDILKGLGVSVSELNGLQPEQIFNRMVAALASVDDETTRAALATDLF
                     GRSGTQLLPLLADGTEGLEALKQEAREAGVVFSQDAAAAADAFGDDMLRLEREIQGVK
                     YQIASELLPALTPMIDEFQSWIDQNGAEFARDTAEAIAGVAEKTSELVGWLGDVASFG
                     PIRVAIDVAANMSGDELAIATTALLVGSRFGPIGLGLGALTAGVAAITSGQPTERPIG
                     GPAGARPAGAPAPVGVRAGGSGSNFTPSVPSGMSDEDAFLAAEEALARAAGWVPNENG
                     PITPPRASAGFAAGRGAGGVSTARGGGAGGAKKAVEELTEAERALARIREDQAAEIER
                     AYVTGGIEAVNALRQLHAEQDATWEAMKAQAREDGYELTDTARDAWQQMLEDQKAANE
                     RMNEENERAFEELERIRQDAIDDMLEAEKKAAEERKRILDDENQDVFNRTRQLWAAAG
                     PNLSTGNALSLARQAELGAILNTNVNNPGLDRGYTIVNYGTQNLALPNVVAGQPLMPS
                     TADSLP"
     gene            13252..15624
                     /locus_tag="VEA63_07040"
     CDS             13252..15624
                     /locus_tag="VEA63_07040"
                     /inference="COORDINATES: protein motif:HMM:NF041539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor R domain-containing protein"
                     /protein_id="HYD83789.1"
                     /translation="MISILTFDGHAINDGTNYDAWFDVGDSPYSRQITPVIQRPLSRI
                     PRYMRRDIQERIIPVHVSIKTDLAANREQLEEWFAPGEAGELVVAYEGTDRALTCVVT
                     RFISYDGSANRFTAVLLAADPEWHSPSVQNVSQQQTASGATWDVTNDGNAVESRAVIT
                     LQPRTAKVAADAYLYKREVIVANRVARAFANYALDVTDSGLDHAALVTATKSQADGDD
                     VRVRVDGVEVPRWFHEHASHDANSAATRVWINISLSPGKFAELLADVTAGTPADGSDL
                     EVVRGGTSGWPRSGAFLIDDEIVEYDGVSESNANGRASFLNIKRARRGTAAASHSAGD
                     ILYWVERRVQIVYGWSAATAPDAFPERKPMLDFTSSTLTNEQHEYLDFAHETDPRSMQ
                     WARTYLSRDDQGQLVRAPSGTPAAAMTLEYDQDGASVGYPNYNAWTRDFPSKTDGNIA
                     ITRVVDNTMALQVIGTDEEGNEVRGTPANNGLLLGPLSSGSVNHDPGDVVSLLFWGVN
                     QLIGASRAVALAAGTAALLDTDSAANAQQFTTGDEPMMLKSVSVWAGGSNESTTLRIM
                     PDNAGAPAGEAASLGSASLNLSTTAGIATATFTTAIRLEPNTTYWIGIETGAGNSWHY
                     TNGSYSGGPARINGTNYAYRRYEFKITGYPAPPSIAYERFDSNCLADDQDVAQVTALT
                     VPLLATGVPYVAVGSEQAAYWLNGTLTNTLTAQSVTFDLICALLDEIEIDVGARTILN
                     VTQGESVFASAQFSDPENWIDIFSGANTFSYDEEGIARVDVTISFRDRWH"
     gene            complement(15621..15956)
                     /locus_tag="VEA63_07045"
     CDS             complement(15621..15956)
                     /locus_tag="VEA63_07045"
                     /inference="COORDINATES: protein motif:HMM:NF024784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HYD83790.1"
                     /translation="MVAKAFLGGPPTNTHQVNHIDGDPTNNRVENLEWVTPSANQIHA
                     ARTGLSNPVANLRFGAQHHSAKLSEEDVRTIRTLLGSMSQQAIADRFGIGQVSVSRIA
                     LGKNWKHVV"
     gene            16172..>16633
                     /locus_tag="VEA63_07050"
     CDS             16172..>16633
                     /locus_tag="VEA63_07050"
                     /inference="COORDINATES: protein motif:HMM:NF025464.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heparin lyase I family protein"
                     /protein_id="HYD83791.1"
                     /translation="MPMIHNTSYEVGDYIPAEQFGDGCVHAVRYHAAGVTQDSLGPSP
                     TPFNGSRTVRFACKTGDPPWTPSGSALGYRSEHEHDDHELSSSGTYCWYGWALYLNAD
                     WKIGSEGWRILTQWHLGSGQPALKLVYDVASAGGWCWQRRTTTNLNQMITPI"
BASE COUNT         3121 a         5345 c         5524 g         2643 t
ORIGIN      
        1 gtcagtttct ctccccccga aagaaagggg gggaggggtg cctaggcgag gtcgccttga
       61 actcgcgctc gctcgcgcag ttcggacggc tcctgtcctg ccgatggacg aggccgcggt
      121 cgaattggcg cgtacttacg ccgcctcgat tgacgcgaat cctctgatgg tggggcaact
      181 tggcggcggg ctaaaggcga tgcttcaaga gttgggcatg acgccgaaag cccgcgcgga
      241 cctgatgggc ggtaaggttg cgccggctgc gaatcccagc ctcgacgagc tacgcgcacg
      301 ccgcgagcag cgcgcgaggc agcgatgaac tgtaagcacc cacttggctg cgagagacca
      361 gtccgggcgc agggctggtg taatgcgcac tatctccggt ggaaggcaac cggagatccg
      421 ggcgtggcgg agatcaagcg ggttcgtcca accggcgagc cgtgcgcagc ggaggggtgc
      481 gcccgccctc gaagcaagcg ggaatggtgt ggcacacact acgaacggtg gcgcgtccat
      541 ggagacgcct caatctcgct cgttgcgcgc ggaaatccct catacctagc gctccataaa
      601 caggtttacc ggcgcaaggg caaagctagc gagcgccaat gtgcgcactg cggctctgct
      661 gctcagcaat gggcctacga ccaccttgat ccgaatgagc ggatcgcgcc catcaaggtg
      721 cgcgacgagg tattcgacct ccgctacagc caagaccctg agcactacat accgctctgc
      781 gttccgtgcc accgccaatt cgaccgggcg gtggtcgcat gatcgcgcag gcgccggcgg
      841 tcatgggccg cacgtatccg agaattgcta cggctcctct ggtaaagggc gaacctggac
      901 cgtgcggctg cggctgcgcc ttatcgccgg ccacgtccta cggattcgat gtagaggact
      961 tcgccaggga cgtactcaac aggcctctcg acccgtggca gcggcacctc gtaatccacg
     1021 cgggagagtt actaggagag gagggaaatg gcgggctgtc ttcgttgcga aggcccagat
     1081 tcaggcaagt cctggttctg gttgcccgcc agagaccgaa cggtaagacc gagctcctcg
     1141 tgatcctcac cctgtattgg ctctacgtcg accgtgttcg gctggttcta ggcacatcaa
     1201 ccaacctcga tactgcgcgc gagtcatggg agaaggcagt ctcgctcgcc gaggacaacc
     1261 caactctggc ctctgaggtc ggggatgttc ggcgtacgaa cggtgagcag acactgacca
     1321 cggtgtgggg tggtcgatac aagatcgcgg cctcaaaccg gaagggtggt agaggcctca
     1381 caatcgaccg cctcgtgctc gatgagttgc gtgagcacgc tcactgggac tcgtacaacg
     1441 ctgctgttcc agcaatgaac gccgtccccg acgccacggc cttcttcatc tcaaaccaag
     1501 gtgacgacaa gtctcaggta ctgaacgcgc tgcgcgacca ggcgttggag ggaagcgacg
     1561 aacgcttggg gttgttcgag tggtcgtccg aggacggcat ggacccgaca gatcctgagg
     1621 ctcttgcgat ggctaatccg agcttagggc ggcgcatttt tctcgacacg ctaatgggtg
     1681 atgcgattcg tgcgaagcaa gccggcgggg aacagttggc ggacttcctc actgaagtgc
     1741 actgcagacg agtgccactc ctgaacccag ctatcgactt ggacgcctgg tcgcgcagct
     1801 acagcgccga gaacagcctc gaggcgttca aggacaagct cgccctgtgc gtcgatgtct
     1861 cgttcgacca gacgcacgcg acgctctacg ccgccgcggt ggatggcgag cgcgtgcgcg
     1921 tcgatcccgt ggcggcatgg gaggggcagg acgccatctc ccagatgtcg tcccagttgc
     1981 cggcgctggt cgagaagatg aaaccgcgcg tgctcggctg gttccccgac ggaccggccg
     2041 cagcagcgct ccccgcgctg actccgcgtc agggctggcc tccggcaggt gtgacggtcg
     2101 aggcgatccg ccaggtgacg gcggtgtgca tgggcttcgc cgagcaggtg cggtcggaca
     2161 aggtctacca ctcgggcgac ccgttgctcg acgcgcacgt gcgggccgcc gagaagctct
     2221 cgtacggcga cggctggcgc ttcactcgac gcaaggcggg gcacgtcgat gcggcgtacg
     2281 ccgctgcggg cgcagtgcac ctcgcgcgca cactccaaga cgagtcagaa ccgatgatct
     2341 tctgatgacg agggatgcgc tggaatggct cggagcgatg ctagtggtgg tgggagcggg
     2401 catggcctat ctccctctcg gattccttgc agcgggattg tggctgctgt gggccgcgaa
     2461 cgcagacgcg atcgcggtac tgatgttcgg tacgggaggc gaagatggca ccgacgctga
     2521 gtaatttttc cacccgcgtc cgtacgcgtg gtgcaaatcc agtgacgcag cgacagacgc
     2581 cccgatggtc gcccagttgg tttacggggt tatcggggcg cacattcgat gtatcgcaag
     2641 ccatgagtgt tccagcattc ggccgcgcgg ggaacatcat cgccggaacg attgcggggt
     2701 tccccttaca catctacgag tccggacagg acgggcagcg tcgattggcg caggacgact
     2761 ccaaccggtt tctgtggggc gtcggcgggc cgcgtagcaa gaacaacgac cggccactcg
     2821 ggcctaaccc ggaggttcca gcgtttgtct tttggaaggt agtggcgact cacaagttct
     2881 ggcgagagaa cgtctacctc tttgtggttt ctgataatgc ccggcgtact ctggaactct
     2941 ggccgatcga tcctgttcgc gtgcgggtcg ggcgcctcgc agacggccgc aagatattcg
     3001 aggtcgatgg caaagaggca tacctcgact acctccacgg aggagagatc gtccatgtcg
     3061 tcggggactc agaggatggg ctcgtcggcg taggaccact acagcgctac gccgacacct
     3121 tcgagacggc gatttcgacg gagcagtacg cctcccggca gttctcaaac aacacgaatc
     3181 cgagcggcat cctcaagacc gaccagcaac tgactccaga ccgcgccgag gaactccttg
     3241 cgcgctggga ggcgcgtcac gctggcgggg taaacgctgg ccgccctgct gttctgtcga
     3301 acgggctgga atggcaaacg atccagttca acccggaaga gactcagatg ctcgagtctc
     3361 gcaagttcgg cgtgttcctc atggagatcc tgagcggcgt cccgccgcac ttgctgggcg
     3421 acgtagaacg gtctacttcg tgggggagcg gcatcgaaga gcaatcaaga aatttcctga
     3481 cgtacacgct ctcgaatcac tacattccga ttgaacagac gatcacgatg gcgttcctgc
     3541 cgaaggatcg ctttgcgttg ttcaacccgg acgccatcct gcggccggac accttcaagc
     3601 gggctcaggt ccacgagatc atgatccgct cccgcatggc tacgccaaac gagcgccgtg
     3661 cctacgagaa catggagccc atcgaaggcg gcgacgagct cgctgctccc ccgaacgcat
     3721 ccctccccgc accgactgaa cgtcgttcaa ctgaggaggg cgagtgatgg gagaacagat
     3781 caccgaacag atgtggggct tgcacgcgga tcgcgaggct ccgtgtgacc gctgctacgc
     3841 gatgatcagc atggtcatag cgggtcagtc cacttggcag aacatcaacg atctccatca
     3901 cgactgccgc ctagcggcga tcaatgttgg cttgatcagg tgggttccgg tgcagcgaat
     3961 gtcgctctct gatgccggcc aactccgaac gctgatcgag gcgtttgagg agggcaacga
     4021 atgatggggg tacagacaat cccggcggcc atcgagcaga agatcgaggc gttttgcggc
     4081 cagaagaaat cggggcggtt catcctcgac atcagcgagg gccgcatcgt cggctgggag
     4141 attctcgaaa agggccgcat ccgtggatga ccgctgcgag tgcggacact cgatcgagcg
     4201 gaagcacggg ccgtacggct gccagtcgtg tcctccgaac aagtgcctgc gcgaaggttt
     4261 gcgtccggtc ggataatgtt gacgcgcgta ctcgggtgag ggcatactag cattaggtaa
     4321 taaggttcct cctacgcccg ccccaggcgg ttccagcgga gcccttcgag cgatgcacat
     4381 cgctacgggg gctccgcttt gtacgacaca agggcgttcc tctccgacca accctgggcc
     4441 gtagacctcc ctcacctcga gagggagctt tctagccgac tcgaaatcct caaggcaatc
     4501 ggccaaccgc acgcggagca acgcgtccca ccgccccgcc tgccgcgcgg tggctctggc
     4561 gtggcagtcc tctcaatccg cggcccgatc acgcaccact ccgaaggcgg ccttctagcc
     4621 gcgctcttcg gcgagacgac gtacgactcg atcggggcgg agtttcgcac ggcgatggcg
     4681 gacgaagccg ttcgcgcgat cgtcctcgac atcgactccc cgggcggcac cgtcgccgga
     4741 gctcccgaac tcgcagccga gatctaccgc gcgcgcgggc gcaagcctgt agtcgcgctc
     4801 gccaacgcgc acgcgatgag cgcggcgtac tggctcggat cgtcagccga agagttctac
     4861 atcacgcctt ctggcgaggg cggcagcatc ggcgtgatcg cctcgcacat gaacctctct
     4921 ggcgccgccg agaaggccgg catccagccg accatcctct ccgccggcaa atacaaggca
     4981 gaggtcaacg agttcgagcc tctgaacgac gaagcactgg ccgccgcgca gcatcgcatc
     5041 gaggccgcat acgacctatt cgtccacgac gtagcgcgca accgcggcgc gaccgaggcg
     5101 caggtgcgca atggctacgg cgaaggccgt tacttggtcg cgaaggacgc ggtcaaggct
     5161 ggcctgttcg acggtgagcg cacgttcgag caggtgcttc gcaacatcgg tcagtcacgc
     5221 tcatcgtctc ccgccgctgc gctcgaagag accgagcgcg ccgcagagtc cattccattc
     5281 ggagttcacg ccacgcaggc gctagctgcc atgcaggcgt tcatctcccg tgcgcaagcg
     5341 ctagctgctc tgcgcgcgga ggagggccgc cccctcggcg agtccgcacg aaacgcactg
     5401 ttctcattcg ctgaggcggt cgcggagcaa acccagctag tcgatcaact gattcgcaac
     5461 cagccagacg cgcgagcgcg tcaggacgcg gaagcgttcg ctcggttccg agtcgcgttg
     5521 cttcgagacg aggccgctgc cacgcagcag gcactggaga tgaacgcacg atgaccacac
     5581 agactcgggc ggaccaggcg cgcgagcgcc tcgtcgaggc cacggcagtc ctgatcgagg
     5641 aactcgccaa gtacgagaac acacctccga ccccggagca gttggagcac ctgaacgaac
     5701 tgaaggcgtc gaaggacaag gccaaggctg acttcgatga ggtcggcaag ttcgaggctc
     5761 tccgcgtcga cgccgcgacc atgcaggact tcctcaagca gccggccaac cgcccgccgc
     5821 acgcggacag cggtgagccg atgcgcgagc gccgcgggcg catctcgccc aagagcctcg
     5881 gccagaagtt cgcggccgac gaaaactacc aggcatggct gaagtccatc gcgccgaacg
     5941 gcaagatccc cgacagcgcg cgaatcccca gctccccggc gttcgctttc ggtggtctga
     6001 acgacctgca gccgatggcg gcgctgctga ccggtgcgtc gtccacatcg ggcggcgcga
     6061 tgatctacga cgacaactac ccaggcctca tcggcctcgg tcgtcgcgaa ctcaccatcc
     6121 gcgacctggt cacccccggc cagacggaca gcgacaccgt gacgtacgtc cgcgtcacga
     6181 gcgagacgaa caacgcggct cccgtggcgg aggcaaccgc tactggtgac ggctccggcg
     6241 tgaagcctga gtcggcgatg gcgttcgagc gcgtctcgac gaccgtgaag acgatcgccc
     6301 actggattcc cgccacgaag gcggcgctgg ctgacgctgg tcagttgcgc acgctgatcg
     6361 acgccttcct gcgcgacggc ctcgaggaag agctcgaaga ccagatgctc accggcgacg
     6421 gttccggcga gaacttcact ggaattttca acacgaccgg cacgcagacg caggcgtggg
     6481 acaccgacat cttcgcgacc acccgcaagg cgattacgaa gatgcgcacc gtcgcgcgtc
     6541 gccgtccgac cgcctgggcg atgctcccgg ccacgctgga gttcctcgac ctccagaagg
     6601 acggcgagga gcgttactac aacggcggcc cattcgggcc tgctggccgc acgctctggg
     6661 gctacccgat cgtcgagacc gaggggctcc cgacgggctt cgcgctgctc ggagacttca
     6721 agcgcgccgt gctctgggat cgcgagcagg cgtcgatcca ggtctcggac tcgcactcgg
     6781 acttcttcat ccgaaacctc gtcgcaatcc tcgcggagat gcgcgcggcc ttcggtgtgc
     6841 tgcagcccaa cgcgttcgtc gaagtggacg tggtcgccta acagggcggc gcgaccggaa
     6901 gcaggagatt cgagatgagc gacacgctca atggcgcggg cctcgaccga gtgtccgtga
     6961 agacggcgaa ctacaccgtc gtcgccgcca cagacaacgg caaggtcttc accaacgagg
     7021 gcgcggcggg ggcgatcacg ttcgccctcc ccgccgcaac ggtgggccag cgctacggct
     7081 tccatgtgat ggcggcgcag gaactgcgca tcgacccgaa cggcaccgag accatctcgc
     7141 tgcccacggg cgtgcagcag gccgccggca agtacatcgg tgccgacgca gcgggcgagt
     7201 acatcctcat ccgctgcgtg aaggcgggtc agtggaacgc cttcggcgct gtcggcacct
     7261 ggacggctga ggcctagcag atgccagaca cggtagcccc gttcacgacc acgacgcagg
     7321 cgctgccccc ggtgcctgtg cgtgtcgtgc aacggggcta ccgctgtccg atctgcaatc
     7381 gactgaacta cgcctgcacg acagaggagc acgaggcatg acaaccgaac gaccggtcac
     7441 gcgggtgaac accgcagacg gcaagaccac gatcaacgtc cgcaacgaag agctcgagaa
     7501 gtacctcgcg gccaaccccg gcgccgaggt ggtgcagccg gagcgcactg cgactcgctc
     7561 gcgtacgcgc gcgcaggccg aggacgcgtc caccgaggac gccgacggcg accaggacga
     7621 gcaggaaacg gacggcgacg acggtccgcg gcgttccccc cgcgcccgca cgactcgtcg
     7681 cagctagcgc agggaagacg gtggcgagtt ggaactgctg gacccgtatg tctcggtgga
     7741 cgagtaccgc acccgtgcgg ggtcgctcgc cattgcagac gacccgaccc tggcagcaca
     7801 actcgtcgcc gtctcccgcc tgatcgagga agtcctcaag gtcgcgcccg gcgcgttcaa
     7861 cagcgtcgag ggcacgcgtt acttcgacag cgacggcacc gcgactctcc ggctccggga
     7921 cgcgcagggc aaacagcact ttctgcagtc cgtcgatgcg aacgcgatcg gcgtcgacaa
     7981 ctcctccagc ggagcgttca cggactacgc gctcgacttc gatgacgact gggtcgtcgg
     8041 agtaggcggg ctcgggcagc cgtacgaggc gatcacgatc agcccgatca cctcggccgc
     8101 cccgtactac tggccgcgcg gcgtgcgcac cgtctcgatc actggtgtct ggggctggcc
     8161 gtctgtgcct ggtgtcatca aggaacttgt ggtcgggctc gcacgcgaca tgcggcaggg
     8221 gcaactgacg gggtcgggcg ggagcatcgc tgacttcgag ggcttcaacg gtcgcgcact
     8281 gtcgccgggg acgtacttct ggtggaccga ggccaagcgc ctgctgtcta gagaactgac
     8341 tgcattcgca tgagttggct caccgcgcgc gccgcgctcg ctgcactcct caatggtcag
     8401 gccctgcagg tcgagggcta ccgcgaagag acgctgacgt gcgtcagcac gccgaaggcc
     8461 atgcccgacg cgccggggtt cccgctggcg gtcgtgcgcc ggccggcgtc gcgcgtcgtc
     8521 aggcgatcgc cgggtggcct gcgcaacttg gaagtcaccg ccgaggtcga cgtcatggtc
     8581 gcggcggaaa cgctcgatga agccgaggag cgcctcgagg cgtgggtgca gcacctgatg
     8641 gacctcgtcg gcgcccacct caagttcgac ggtcagacgg acgtgaccgt gaccgggcag
     8701 gagttcggcg agttcgggtc gtacagcgac caggcgaacg cctcgccgtt cggcttcccg
     8761 atgcaactga cggtcttgct taccgacacg gaaacgcgcg gagcgtagga ggcagcacat
     8821 ggcgtggaag ttcaagggcg aagggctgcc gctgccgggc atcggggcgg ggccgttcac
     8881 agacgaagag ttcgagatcg cggtctcggt gtacgaggcg cagttctcgg atgaccagaa
     8941 gggcgcggtc aaggcgtccg gcctctacga gcggacgtcg ctcgagaagg tcgcggacga
     9001 actgcctgac acgccgccgc ccgtcgtggc agaggcaaaa gccgacgagc agccgcggcg
     9061 ccgcggtggg aggactgagt aaatggcgat cacagcgtcc ggtctgaagg tctaccaggg
     9121 cgggccggag gtcactcctg gcacgcccgt agcggcgacg cggaagttct cgctagagaa
     9181 cctcgactgg acgccgcagg acgaggtcta ccgcccgcgc ttcgcgaacg gggtactgct
     9241 cgacaaccgc ggcggcgaga cggtcatctc gcgcggctcg gagttcacgg gcgaagggcc
     9301 ggtcatcccg gacgagctcc accgctgggc gtcgatgtgc atcgcggctg acgtgatcac
     9361 gggctcaggc ccgtacacgc acaccttcac gcgcaacccg gtcgccaacc cgacgctgaa
     9421 aacgtggact ctggagcggc gcctttcgga cggatcaaac gcgatcgaca acgaatggag
     9481 ccatgcgctc ctgcgctcgc tgacgatctc ggccgccatg gacgcgccgt tgcgtctgag
     9541 cttcgcgggg atcgcgcggc gcgtgctgga ctcaacactg accgccgcgc tgtcgcaggt
     9601 cacgacggag attctgccgt cgtcgctctc gaccgtgttc atcgacccgg cgtgggctga
     9661 cctcggcgag acgcaggtca cgcagcaggt gctgtcgttc tccgtcacgt tccgctcggg
     9721 catcaatccg gtaaaaaccg ctgacggtag aacggatttg gacttcggca tcgtcacggt
     9781 caatgacgag gaagtcggca tcgacatctc gatgcgcctg atggtcaagg cggactctgg
     9841 ccagtacgcg acggaaaaga ctgcggccga ggcgcagagc ctccgcgccg tccgcatcgc
     9901 cgtcgctggt gcgggcgaca ccgagatcac gatcgacgcg ctctgcaagc acgtcgctgg
     9961 gtcgctgttc aaggtcgacg agcaggacgg atacgaggtc ttcgacatga acctcgtgtc
    10021 gagcactgac ctcaccaatg cataccgcct ggtcgtgatc aacgcggcct cggcgctcta
    10081 gggggaactg agtggcggag aagcaccgga tcaagttgga cgacctgctc ggcgaggact
    10141 acgcgggcga gtgggtggac atccacaagg cgcgtacgtg gggcgcgcgg ctcaaggtgc
    10201 aggacgcggc gcgcggcgac gtgtccgact acaagctcac cgtgcttgac gtctcgatcg
    10261 tcggctggtc acgcagcgag ccgcatgagc gctcggcata cgaagcgctg gccgaggagg
    10321 caggagaggc gctgttcgag gcgattaccg gctactacgc cagccggagg cgtgccgcag
    10381 atgcctcgaa aagcgctgtg gctgcagggg ctgacgaacc cggagagcgt gccgcctgaa
    10441 ctggcgtacg tggctgtagc ggaacgcttc ggctgcgacc cgcaggcggt gctcgagtgg
    10501 gacgaagaag tggcggaaga cgtggcgctg ttcctgcgga tctcgaacgc gaactcgcgc
    10561 gccgcggctc aggtgcgaag gggtctccgt ggctgatcgc atctacgtca aaggcctcaa
    10621 ggagatgcgc ggcgcactgc gcaacctcgg cccggagttc gtgaaggaac tccagcgcga
    10681 gaacaaggcg gtcgcaaacg agatcgggca ggaagccggc cgcgcgttcg ccgcggcgca
    10741 tccgggcggc tcaggtcgca gcgccgccac gtatcgcggt ctcgcaacgc agaccagcgc
    10801 gaaggtcgct gctggctccg cgcgtcgcct ccatgtgctc gggttcgact tcggctcgaa
    10861 ccagggcaag aacaagaagc agtttccgcc gtacgtgaag ggcgggcggt tcctctatcc
    10921 gacgatccag cgccgctcgg ctgacctgcc agagcggtac gggaaggtgc tcgacaaggt
    10981 tgggcgcccg gtgttccccg gtgggtcgcg atgacttacg gggtgcagaa gttggctgtg
    11041 ttgctccacg cggcgcacat cgcgtcgatt tcctcgcgcc cgcagatgac cgcgtcggtg
    11101 atgaggcgga tgcagggctg gctcacgagg ctctcccagc cgagcttcat caggactacg
    11161 tcgaaggcgc ccatgctcca gacgatgaaa agccctacgc ccagcagtag agcgagggcg
    11221 gggatggcct ttgagggtgc cggggtgagg gtttgggcgc tttgcacggg cgttcagtct
    11281 agcagcacga aggcggccgg gaatggctga ccgccagctc accgtaacaa ttttgggaga
    11341 tgctgccgac gccgtgcgtg ccttgaagca gatggacggc gaactcggca cggttgagac
    11401 cacgacgacc cgcgttggcc ggacgatgca gaatgccttt gccatcggcg cgacggcgat
    11461 cgcggctggc acggcagcgg cggcggcagg cgcgggggcg gtactcaacc tcgcgaactc
    11521 ctatgccgag gcgggttcgc aaatcttcga cctgtcgcag cggacggggt tctccaccga
    11581 ggctctctcc gaatggaagt acgcggctga ccagtccggg gccagcatcg aaggcctaga
    11641 ggttggcatc cggcggatgt cctcgacggt cgtggatgca ggcaatgggc tcgacagtgc
    11701 caaagacatc ctgaaggggc tcggagtctc ggtctcagaa ctcaacggcc tccagccgga
    11761 gcagattttc aaccgcatgg tcgccgctct cgcctccgtc gatgacgaga cgaccagagc
    11821 ggcgctcgcg actgacctgt tcggacgctc cggcacgcaa cttctgccgt tgctcgctga
    11881 tgggacggaa ggcctcgaag cgctgaagca agaggctcga gaggcaggtg tcgtcttcag
    11941 tcaggacgcc gccgcagccg cggacgcatt cggcgacgac atgctgcggc ttgaacgaga
    12001 gattcagggc gtcaagtacc agatcgccag tgagttactc ccagcgctca cgccgatgat
    12061 cgacgagttc cagtcgtgga tcgaccagaa cggcgccgag ttcgctagag acacggccga
    12121 ggccatcgct ggcgtagcgg aaaagacgag cgaactggtc ggctggctgg gggacgtcgc
    12181 gagcttcggg ccgatccgag tggcgatcga cgtggccgcg aacatgagcg gcgacgagct
    12241 cgcgatcgcg actacggctc tgctcgtggg gtcgcggttc gggccgatcg ggttagggct
    12301 cggcgcgctc actgcgggcg tcgcagctat tacctccggg cagccgacgg aacgcccgat
    12361 cggcgggcca gcaggagcgc gccccgcggg tgcccccgcg cctgttggcg tccgcgccgg
    12421 cggttcgggc agcaacttca cgccttcggt tcctagcggc atgagcgacg aggacgcctt
    12481 cctcgccgct gaggaggcgc tcgcacgcgc ggccggctgg gttccgaacg agaacggccc
    12541 aatcacaccg ccgcgcgcat cggccgggtt cgccgcaggc cgtggtgcgg gcggggtgag
    12601 caccgctcgc ggtggcggcg ccggcggtgc aaagaaggcg gtcgaggaac tcaccgaggc
    12661 cgagcgcgcg ctcgcccgca tccgcgagga ccaagctgcg gagatcgagc gcgcctacgt
    12721 cacgggcggc atcgaagccg tcaacgcact gcgccaactc cacgccgaac aggacgcgac
    12781 ctgggaggcg atgaaagccc aggcccgtga ggacggctac gaactgacgg acacggctcg
    12841 cgacgcgtgg cagcagatgc tcgaagacca gaaggccgcg aacgagcgga tgaacgagga
    12901 gaacgagcgc gccttcgagg aactcgagcg catccgccag gacgcgatcg acgacatgct
    12961 cgaagcggag aaaaaggcgg cggaggagcg taagcgcatc ctcgacgatg agaaccagga
    13021 cgtcttcaac cgcacgcgcc aactctgggc cgcagccggg ccgaacctct cgaccgggaa
    13081 cgcgctctcc ctcgcgcgcc aggcggagct cggcgcgatc ctcaacacca acgtcaacaa
    13141 tcccggcctc gaccgcggct acacgatcgt caactacggc acgcagaacc tcgcgctgcc
    13201 caacgtggtc gcgggtcagc cgctcatgcc gtccacggcg gacagcctac cttgatctcc
    13261 atcctgacct tcgacgggca cgccatcaat gacggcacga actacgacgc ctggttcgac
    13321 gtaggggact cgccgtactc gcggcagatc acgccggtca tccagcgccc gctctctcgc
    13381 atcccgcgct acatgcggcg ggacatccag gagcggatca tcccggtcca cgtctcgatc
    13441 aagaccgacc tcgccgcaaa tcgcgagcaa ctcgaggagt ggttcgcacc gggggaggcg
    13501 ggcgagctcg tcgtcgccta cgagggcacg gaccgcgcgc tgacctgcgt cgtcacgcgc
    13561 ttcatctcct acgacggctc cgcgaaccgc ttcacggccg tactgctggc cgctgacccg
    13621 gagtggcact cgccatcggt acagaatgtc agccagcagc agacagcgag cggtgccacc
    13681 tgggacgtca cgaacgacgg gaacgccgtc gagagccgcg ccgtgatcac gctccagccg
    13741 cgcacggcga aggtcgcggc ggacgcctac ctctacaagc gcgaggtgat cgtcgccaac
    13801 cgcgtcgcgc gcgcattcgc gaactacgcg ctcgacgtga ccgacagcgg cctcgaccac
    13861 gccgcgctag tcacggcgac gaagtctcag gctgacggcg acgacgtccg ggtgcgtgtg
    13921 gatggcgtcg aggtgccgcg ctggttccat gagcacgcga gtcacgacgc gaactcggcg
    13981 gcaacgcgcg tctggatcaa catcagcctg tcacccggca agttcgcgga gctgctcgcg
    14041 gacgtgacgg caggcacccc agcagacggc tcggacctcg aggtcgtgcg gggcggcacg
    14101 tcgggctggc cgcgctcagg cgcgttcctg atcgacgacg agatcgtgga gtacgacggg
    14161 gtttcggaga gcaatgcaaa tggcagggcc agttttctaa acatcaagcg cgcgcgccgc
    14221 ggtacggcgg cggcgagcca tagcgcaggc gacatcctgt actgggtcga gcgccgcgtg
    14281 cagatcgtct acggctggtc ggcggctaca gcgccggacg ccttccctga gcggaagccg
    14341 atgctggact tcacgtcgag cacgctgacg aacgagcagc acgagtacct cgatttcgcg
    14401 cacgagaccg acccgcggtc gatgcagtgg gcgcgcacct acctcagccg cgacgatcaa
    14461 ggccagcttg tccgtgcgcc gagcggcact cctgccgccg cgatgacgct cgaatacgac
    14521 caggatgggg cgtctgtcgg ctatcccaac tacaacgctt ggacgcgcga cttcccctca
    14581 aagacggacg ggaacatcgc gatcacgcgc gttgtggaca acacgatggc cttacaggtg
    14641 atcgggacag acgaggaagg caacgaggta cggggcacgc ccgccaacaa cggattactg
    14701 ctcgggccgc tgagttcggg ctcggtcaat cacgaccctg gcgatgtggt gagcctgctc
    14761 ttctggggcg tgaaccagtt gatcggggcg tcgcgtgctg tagcgctggc ggctggcact
    14821 gctgccttgc tggacacgga tagcgcggcg aacgcgcagc agttcacgac tggcgatgag
    14881 cccatgatgc tgaagtcggt ttccgtctgg gcgggcggca gcaacgagtc caccaccctc
    14941 cgcatcatgc cagacaacgc tggggcgccg gccggggagg ccgcctcgct ggggtctgca
    15001 agcctcaacc tcagcaccac cgcaggcatc gcgacagcca ccttcactac ggccatccgc
    15061 ctcgagccga acacgacgta ctggatcgga atcgagaccg gcgccggtaa ctcgtggcac
    15121 tacacgaacg gcagttactc gggcggacca gcgcgcatca acggcacgaa ctatgcctac
    15181 cgccgctatg agttcaagat cacgggctac ccggcgccgc ccagcatcgc gtacgagcgc
    15241 ttcgactcga actgcctcgc agacgaccag gatgtggcgc aggtcacagc actcactgtg
    15301 ccgctgctcg ccaccggcgt cccgtacgtt gcggtcggca gtgagcaggc ggcgtactgg
    15361 ctgaacggca cgctgaccaa cacccttacg gcacagagcg tgacgttcga cctgatctgc
    15421 gcgctgctgg acgagatcga gatcgacgtc ggcgcgcgca ccatcctgaa cgtcacgcag
    15481 ggcgagtcgg tattcgccag cgcgcagttc tctgacccgg agaactggat cgacatcttc
    15541 tcgggcgcga acaccttctc gtacgacgaa gaggggatcg cgcgtgtcga cgtcacgatc
    15601 tcttttagag acaggtggca ctagaccaca tgcttccaat tcttaccgag tgcgatgcgc
    15661 gatacagaga cctgaccgat gccaaatcga tcggcaatgg cctgctggct catgctgccg
    15721 aggagggtgc gaatcgtgcg gacatcctct tcagagagct tggcgctgtg gtgctgggct
    15781 ccgaaacgaa ggttcgcgac tgggttagaa aggcccgtgc gcgcagcatg gatctggtta
    15841 gcgctcgggg taacccattc aaggttttcg acacgattgt ttgtggggtc gccgtcgatg
    15901 tgattgacct gatgggtatt tgtcgggggg cctccgagga acgccttcgc taccaaccta
    15961 tgaaccgttc ggctgtattg cttgtcttgg ccgcggagat tgacgtacag gcgtctctcc
    16021 caaacgcccg gacggagcac gcgtggcgca ggcctccctg gcagtactac cacgcgcctg
    16081 acgttcccaa gactgctcac ttcgtacagg gggaactctt cgatctggcg ccactcttcc
    16141 atttctcgat tatacgaagt cggtggcact gatgccgatg atccacaaca cctcgtacga
    16201 agtcggcgac tacatcccag cggagcagtt cggcgacggc tgcgtccatg ctgtgcgtta
    16261 tcacgcggca ggcgtcacgc aggactcact cggcccatcc ccgacaccgt tcaacggctc
    16321 acgcactgtg cgcttcgcct gcaagactgg cgacccgccc tggacgccca gcggctcggc
    16381 gctgggctac cgctcggagc acgagcacga cgaccacgaa ctgagcagct cgggcacgta
    16441 ctgctggtac ggctgggcgc tctacctcaa cgccgactgg aagatcggct ctgagggctg
    16501 gcgcatcctc acgcagtggc acctcggctc cgggcagccc gcgctaaagc tcgtctatga
    16561 cgtggcctct gctggtggat ggtgctggca gcgccgcacg acgaccaacc tgaaccagat
    16621 gatcacaccg atc
//
LOCUS       DASPLT010000673         4978 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_233103, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000673 DASPLT010000000
VERSION     DASPLT010000673.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 4978)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4978)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4978
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_233103"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..390
                     /locus_tag="VEA63_07055"
     CDS             <1..390
                     /locus_tag="VEA63_07055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GxxExxY protein"
                     /protein_id="HYD83792.1"
                     /translation="GWTQMNTDELPESGLTREIIGAAFEVLNEVGHGLHEKPYENALV
                     IELGLRGLVCDQQRQFDVLFKGFKITDYIPDLIVSGQVIVDAKVIDRITDLERGQMIN
                     YLRITKLRVGLVINFKRPKLEWERIVL"
     gene            433..1800
                     /locus_tag="VEA63_07060"
     CDS             433..1800
                     /locus_tag="VEA63_07060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="POT family MFS transporter"
                     /protein_id="HYD83793.1"
                     /translation="MSQPYLTAPQKISTMPPGIPYIVGNEAAERFNFYGMRAVLVIFM
                     TQFLVNREGQLAPMTESEANKWYHLFLATNYFLPVFGAILADAFLGKYKTIIWLSLVY
                     CLGSIVLAIDHTRTELGVGLMLIAIGSGGIKPCVSAHVGDQFSAENERLLSKAFGWFY
                     FSINAGSLISIPLTPILLHYYGPRWAFGVPAALMLLATFVFWLGRYKFAHIPPAGRAF
                     FRDNFNAEGRAAIGRIALIYVFVAVFWSLWDQSGGEWVLQAEKMDRQVTIFGWSFEVF
                     SSQVQTLNAVFILVLIPVCSYVLYPAIDRVFPLNPLRKIGLGLLVTVSSFLISAWIES
                     RITAGLRPSIAWQVPAYLLLSLGEVMVSVTALEFAYTQAPKRMKSIIMSLYLLAISAG
                     NLFTALVHHFIANEDGTTKLPGATFYLFFAGLCLLTAGVFAIVARSYQEKSYLQDEAT
                     TETAS"
     gene            1918..2781
                     /locus_tag="VEA63_07065"
     CDS             1918..2781
                     /locus_tag="VEA63_07065"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HYD83794.1"
                     /translation="MTRIPITKTPKVYVGGAFIRSESARVFPIHDVDGNFFANIPQCT
                     RKDLRNAVEAAAKAGPGWAKRTAYNRGQILYRLGEMLEARRSEMLDTLVRFGCDAKRA
                     VRGFDTSVDRLVYYAGWADKYEQVLGNVNPVAAPYFNFTVTEPMGVIGVLAPDQPPLL
                     PLISLIAPAIVSGNTVVAVVSEKMPYPAILLGEMLATSDLPGGVVNLLTGFRKELVPT
                     FATHTHLRAIVGAVSAEDRKALELGAAESVKRTRLVDAENADWLGDASQSLYAIRDTL
                     EFKTTWHPVGA"
     gene            2991..4772
                     /locus_tag="VEA63_07070"
     CDS             2991..4772
                     /locus_tag="VEA63_07070"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYD83795.1"
                     /translation="MFAGASVLTGADAPLDRVVLQLKWRHQFQFAGYYAAVAQGYYRE
                     AGLEVELREAASGRDPVDEVLSGRADFGVGNSDLLLLRAEGKPVVVLAAVFQHSPLLL
                     VTRAASGVSDLQGLHDRPIMMIESEKAELFAYFKYEGVDTKRLRVLPHTLRHEEFIEG
                     RVDAMSAYGTDEPYTLRAEGVAFHAFTARSGGIDFYGDNLYALESQLRDHPARVRAFR
                     EASLRGWDYALAHPDEMVNWIFERYPNRHTREHLQFEAAKTIELMHPGVIEVGHMNPG
                     RWRHIADTYAEFGMLPRDFDLKGFLYDPNAQPDLRWVYWALGLLAVVATGSLGWALPL
                     VRLNHRLRVAKEAAEAANAAKSRYLAFLTHEIRTPIGGLVGLVDVMKMENVSALQRQQ
                     VQMVEQAAQQLLQLVDSVLDHSKLEAGRMELNLERVALPDFMRGLCELFQPAAQAKGL
                     SLSYEFYQGVPSSIMTDAMRLRQILANLISNAIKFTPAGGVSLLVEALPVESADVGAY
                     RRHRLFFMVSDTGVGIPPEKQSGLFEPFRQADASIASRFGGSGLGLSISRQLAELLGG
                     QIRLESTPGQGATFTVDIVAEEPPPTE"
     gene            complement(4762..>4978)
                     /locus_tag="VEA63_07075"
     CDS             complement(4762..>4978)
                     /locus_tag="VEA63_07075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HYD83796.1"
                     /translation="TTSGRFITGPGGKGANQAVAAARAGAATRFIGAVRRDVFTAGTA
                     KFGAASAAATARAMAAFARDPLAQPIR"
BASE COUNT          872 a         1349 c         1699 g         1058 t
ORIGIN      
        1 ggatggacac agatgaacac agatgaactg ccggagagcg gacttacccg ggagatcatc
       61 ggagcggcgt tcgaggtgct caacgaggtc gggcatgggc tgcatgaaaa gccttacgag
      121 aacgcgctgg tcatcgaact cggtttaagg gggctcgtct gcgatcagca aaggcagttc
      181 gatgtgctct tcaaaggatt caaaatcacc gactacattc ccgacctgat tgtgtcaggt
      241 caggtgattg tcgacgcgaa ggtcatcgat cgaatcacgg atttggagcg cggtcagatg
      301 attaattatc tccgaattac gaaacttcgc gttggtctcg tcattaactt caagcggccg
      361 aagctagagt gggagcggat tgtgctctga tatccgtgtc catcagtgtt catccgtggt
      421 ttaaaatttt tcatgtctca gccttacctc acagcgccgc agaagattag cacgatgccg
      481 ccggggattc cttatatcgt ggggaacgag gcggcggagc ggttcaattt ttatgggatg
      541 cgcgcggtgc tcgtcatctt catgacgcag ttcctcgtga atcgcgaagg gcagctcgcg
      601 cccatgaccg aatcggaggc gaataagtgg tatcacctgt tcctcgcgac gaactacttc
      661 ctgccggtgt tcggcgcgat tctggcggac gcttttttgg ggaaatacaa gaccatcatc
      721 tggctctcgc tcgtgtattg cctcggcagc atcgtgcttg ccatcgatca cacgcggact
      781 gagctcgggg ttgggttgat gttgatcgcg atcgggtcgg gcggaatcaa accgtgtgtg
      841 tcggcgcatg tcggcgatca gttcagcgcg gagaacgagc gactgctgtc gaaggcgttc
      901 ggctggtttt atttttcgat caacgccggc tcgctgatct cgattccact cacaccgatc
      961 ctgctgcatt attacgggcc ccgatgggcc tttggcgtgc cggcggcgct gatgctgctg
     1021 gcgacgttcg tgttttggct ggggcggtat aaattcgcgc acattccgcc ggcggggcgc
     1081 gcgtttttcc gcgataactt caatgcggag ggccgcgcgg cgatcgggcg gatcgcgttg
     1141 atctatgtgt tcgtggcggt cttctggtcg ttgtgggacc agagcggtgg cgagtgggtg
     1201 ttgcaggcgg agaagatgga tcgacaggtg acgatcttcg gctggtcgtt cgaggtgttt
     1261 tcatcgcagg tgcagacgct caacgcggtc ttcatcctcg tgctgatccc ggtgtgttcg
     1321 tatgtgctgt atcccgcgat cgatcgcgtt ttcccgctga atccgttgcg taagatcggg
     1381 ctcggtctgc tggtgacggt gagctcgttt ttgatcagcg cgtggatcga gtcgcgcatc
     1441 accgccgggt tgcggccctc gatcgcgtgg caggtgccgg cgtatctgct gttatcgctc
     1501 ggcgaggtga tggtgtcggt cacggcgctg gagttcgcct acacgcaggc gccgaagcgc
     1561 atgaagtcga tcatcatgtc gctctacctg ctcgcgattt ccgcgggcaa tttgttcacg
     1621 gcgctggtgc atcatttcat cgcgaacgag gacgggacaa cgaagctgcc gggagcgacg
     1681 ttctacctgt tcttcgcggg gctttgcctg ctgaccgccg gggtgtttgc gatcgtggcg
     1741 cggtcgtatc aggaaaaaag ttacctgcag gacgaggcga cgacagagac ggcgagttga
     1801 acggcttcca atttttaaac cacgaatgga cacgaattca cacgaatgca gaaggatgat
     1861 gaccgtgatt tcggtcgaat ccatgcgcgt ccattcgtga ttcaacccgt ttcaaacatg
     1921 acccgaattc ctattaccaa gactccgaag gtttatgtcg gcggcgcgtt cattcgctcg
     1981 gagagcgcgc gcgtgtttcc gatccacgac gtggacggga attttttcgc caacattccg
     2041 cagtgcacgc gcaaggatct gcgcaacgcc gtcgaagccg ccgcgaaggc ggggccgggc
     2101 tgggcgaagc gcaccgccta taaccgtggt cagattctct accggcttgg cgagatgctc
     2161 gaggcgcggc ggagcgagat gctcgacact ctggtgcgct tcggctgcga cgcgaaacgc
     2221 gcggtgcgcg gtttcgacac gtcggtcgat cggttggtct actacgccgg ttgggcggac
     2281 aaatacgagc aggtgctggg caacgtgaac cccgtggcgg caccgtattt caacttcacc
     2341 gtgaccgagc cgatgggggt gatcggcgtg ctcgcgccgg accagccgcc attgttgccg
     2401 ctgatttcgt tgatcgcgcc ggcgatcgtg agcggcaaca ccgtggtcgc ggttgtatcg
     2461 gaaaagatgc cgtatccggc gattctgctc ggcgagatgc tggcgacgag cgatctgccg
     2521 ggtggcgtgg tgaatttgct gacgggattt cggaaagagc tcgtgccgac gtttgcgacg
     2581 cacacgcatt tgcgggcgat cgtgggcgcg gtgtcggcgg aggatcgcaa ggcgctcgag
     2641 ctcggcgcgg cggaaagcgt gaagcgcacg cggctcgtcg atgcggagaa cgcagattgg
     2701 ctgggcgacg cgtcgcaaag cctttatgcg attcgcgaca ccctcgaatt caagacgacg
     2761 tggcatccgg tcggggcgtg agtggcgagc ggcgagaccc acgaaacggc ggcagtcagt
     2821 gggatgaaga aatcggattc gggctgtcac caggatggtg atacgtccga gcactcgctg
     2881 aacgcttgca ctacgtttcg cttgccgggg taaagcaggt tcaggttact gtgcgcatcg
     2941 tgcggttctg gcttttccga ttcattcttc gtctgatttg tggcggtgcg ttgttcgccg
     3001 gagcgagcgt tctcacgggc gcggatgcgc cgctcgatcg ggtcgtgctg caattgaagt
     3061 ggcggcacca gtttcagttt gccggctact acgccgcagt ggcgcagggc tattatcgcg
     3121 aagcggggct cgaggttgaa ttgcgcgagg cggcgtcggg gcgcgatccg gtcgacgagg
     3181 ttttgagtgg ccgggcggat ttcggggtgg gcaactccga tctgctgctg ttgagagcgg
     3241 aagggaaacc ggtggtggtg ctcgcggcgg tttttcagca ttcgcccctg ctgttggtga
     3301 cgcgcgcggc aagcggcgtg agcgacctgc aagggttgca cgaccggccg atcatgatga
     3361 tcgaatcgga gaaggcggag ctgttcgcgt atttcaaata cgagggcgtg gacaccaagc
     3421 gattgcgcgt gctgccgcac acgttgcgcc acgaggagtt catcgagggc cgcgtggatg
     3481 cgatgtcggc ctacggcacg gacgagcctt acacgctgcg cgcggagggc gtggcgtttc
     3541 atgcgttcac ggcgcgatcg ggcggcatcg atttttacgg agacaacctc tacgcgctcg
     3601 aaagccagct ccgcgatcat ccggcgcggg tgcgggcatt tcgggaggcg agcttgcgcg
     3661 gctgggacta tgcgctggcg catcccgacg agatggtgaa ctggattttc gagcgttatc
     3721 cgaatcgtca cacgcgcgag cacctgcagt tcgaggcggc gaaaacgatc gagctcatgc
     3781 atcccggcgt gatcgaagtc gggcacatga atccgggacg gtggcgccat atcgcggaca
     3841 cgtatgcgga gttcggcatg ctgccgcggg atttcgacct gaagggattt ctttacgatc
     3901 cgaatgcgca gcctgatttg cgctgggtct actgggcgct cggccttctc gcggtcgtcg
     3961 ccaccggatc gctcggctgg gcgttgccgc ttgtccggct caatcaccgg ttgcgcgttg
     4021 cgaaagaagc ggccgaagcg gcgaatgcgg cgaagagccg ctatctcgcg tttttgacgc
     4081 acgaaattcg cacgccgatc ggcggcttgg tggggctggt cgacgtgatg aagatggaga
     4141 acgtatccgc gctgcaacga cagcaggtgc agatggtcga gcaggcggcg cagcagcttt
     4201 tgcagctcgt ggacagcgtg ctggatcact cgaaattaga ggcggggcgg atggagctga
     4261 acctggagcg ggtcgcgctg ccggatttca tgcgcggatt gtgcgagtta tttcagccgg
     4321 cggcgcaggc gaaaggtttg tcgctctcct acgaattcta ccaaggggtg ccatcgtcga
     4381 tcatgacgga cgcgatgcga ctgcgccaga ttctggccaa cctcatctcg aacgcgatca
     4441 agttcacccc ggccggcggg gtctcgcttt tggtggaagc gctgcccgtc gaaagtgccg
     4501 acgtcggcgc gtatcgccgg caccggcttt tcttcatggt gagcgacacg ggcgtcggca
     4561 ttccgccgga gaaacagagc ggattgttcg agccgtttcg gcaggcggac gcgtcgatcg
     4621 cgtcgcggtt tggcggcagc gggctggggc tttcgatttc tcggcagttg gcggaactgc
     4681 tcggcgggca gatccggctc gagagcacgc cgggccaggg agcgacgttc accgtcgaca
     4741 tcgtcgcaga agaaccgccg cctaccgaat aggctgagcc aacgggtctc gcgcgaacgc
     4801 tgccatcgcg cgcgccgtcg ccgcggcgga tgcggcgccg aatttcgccg tgccggccgt
     4861 gaatacgtcg cgtcttacag cgccgatgaa gcgcgtggca gcgccggcac gcgcggccgc
     4921 gacggcctgg ttggcgcctt tgccgccggg tccggtgatg aagcggccgc tggtcgtc
//
LOCUS       DASPLT010000674         1820 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_273883, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000674 DASPLT010000000
VERSION     DASPLT010000674.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1820)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1820)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1820
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_273883"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..742
                     /gene="ispH"
                     /locus_tag="VEA63_07080"
     CDS             <1..742
                     /gene="ispH"
                     /locus_tag="VEA63_07080"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015968 - stringent response [Evidence
                     IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HYD83797.1"
                     /translation="QMMEKLSQEGIREVGDYQSRADLSLAPTAAGENPVMVVRAHGIS
                     PERRTYLKNLGMEFKDATCPDVGIIAGKIRLHAKKGYSTIIFGDKNHPEAIGLMGYTE
                     GRGYCITSKADIDALPPLERVCMVSQSTMFMHEFAELSDYARSKFGEVLVFDTICQAT
                     KDRQTDVVTLAREGAQAIVVIGGHHSANTVKLASLARLQNLPTYHIETAAEIIPDEMK
                     QYETVGVTAGASTPEFLISEVCTKLAAL"
     gene            complement(950..1552)
                     /gene="rdgB"
                     /locus_tag="VEA63_07085"
     CDS             complement(950..1552)
                     /gene="rdgB"
                     /locus_tag="VEA63_07085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009512075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HYD83798.1"
                     /translation="MKLFFASGNAHKVAELQALATASGVPLEIVSARAVGGMPEVVED
                     AGTFVGNARKKAQALRAKLPAGSWVLADDSGVCVDALDGGPGVESAYYAGPEADAVAN
                     LRKLIEVLRDVSEGQRGAHFLCVLLVITGDGEELVFEGVCGGRMLREPRGAWGFGYDP
                     LFVPDGYEQSYAELGDEVKNTISHRARAWARLAEWARAKA"
BASE COUNT          357 a          722 c          437 g          304 t
ORIGIN      
        1 gcagatgatg gaaaaacttt cgcaggaagg cattcgcgaa gtcggcgact accaaagccg
       61 cgccgatctc tcgctcgcgc ccaccgccgc cggcgaaaat cccgtcatgg tcgtccgcgc
      121 ccacggcatc tcgccggaac gccgcaccta cctcaaaaat ctcggcatgg agttcaaaga
      181 cgccacctgc cccgacgtgg gcatcatcgc cggcaagatc cgcctccacg ccaaaaaggg
      241 ttactccacc atcattttcg gcgacaaaaa ccaccccgaa gcgatcggcc tcatgggcta
      301 caccgagggc cgaggttact gcatcacgag caaggccgac atcgacgcgc tcccgccgct
      361 cgagcgcgtg tgcatggttt cgcaatccac catgttcatg cacgagttcg ccgagctgtc
      421 cgactacgca cgcagcaaat tcggcgaagt cctcgtcttc gacacgatct gccaggcaac
      481 gaaagaccgc cagaccgatg tcgtcaccct cgcccgcgaa ggcgcgcaag cgatcgtcgt
      541 gatcggcggt caccattccg cgaatacggt gaaactcgcc tcgctcgcgc gtctccagaa
      601 tctccctacg tatcacatcg agaccgccgc cgaaatcatc cccgacgaga tgaagcaata
      661 cgagaccgtc ggcgtcaccg ccggcgcctc gacgcccgag ttcctcatca gcgaggtctg
      721 cacgaaactc gctgcgctct aagccgagtc cgaggtggaa cccgacagtc ctcgtcgggt
      781 tttttcgcga gcgaaccgct ccgagtgaat gttcaactcg ttggcgctcg ctccgattaa
      841 agatcgcgta gcgatcgccc gatcctagcc ccggcctcca agccggagtt ttccatcccc
      901 acgcacctgc gtcgcgtagc gagcctgaca tcccgcgccg ccctccaact cacgccttcg
      961 cccgcgccca ctcggccaac ctcgcccacg cgcgcgcccg atggctgatc gtgtttttca
     1021 cttcgtcgcc cagctccgcg taactctgct cataaccatc cggcacgaac aacggatcgt
     1081 aaccaaaacc ccacgcgccg cgcggctccc gcaacatccg tccgccacac acgccttcga
     1141 aaaccagctc ttcgccatcg cccgtgatca ccagcagcac gcataaaaaa tgcgcgcccc
     1201 gctgcccctc ggacacgtca cgcaacacct cgatcagctt ccgcaaattc gccaccgcgt
     1261 ccgcttccgg ccccgcatag taagccgact ccacgcccgg cccgccatcg agcgcgtcga
     1321 cgcacacccc gctgtcatcc gcgagcaccc acgatcccgc cggcagtttc gctcgcagcg
     1381 cctgcgcctt ctttcgcgca ttgccgacaa acgttcccgc gtcttcgacc acctccggca
     1441 tcccgcccac cgcgcgcgcc gacacgatct cgagcggcac gccactcgcc gtcgccagcg
     1501 cctgcaactc cgccaccttg tgcgcgttgc ccgaagcaaa aaacaatttc atcgactcgt
     1561 cattgcgcac acccgcctgc caaagcaacc ccgcgccaca tcgccccgtc caccgtctac
     1621 agcctaccgt ccaccgttcc gccgactcac gccatgctga acgtcgtctc gtcgatgaac
     1681 atcttctcgg gatactgcac gagcccgcgc gtcagcacat cctcgccgtg catctcattg
     1741 cgcacctcgc gcaaccggat cgcgttgatc agccgctccg tccgcagtcc gttgaacatc
     1801 ggcttcgcct tctcgtccgc
//
LOCUS       DASPLT010000675         1231 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_172503, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000675 DASPLT010000000
VERSION     DASPLT010000675.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1231)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1231)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1231
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_172503"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..209)
                     /locus_tag="VEA63_07090"
     CDS             complement(<1..209)
                     /locus_tag="VEA63_07090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD83799.1"
                     /translation="MSELSAAAVEDGAGSLTGEETMAGETGGDWLAANGGRSLAQPRK
                     LVSWQRMFVDNEFSLRAKECSVGER"
     gene            211..>1231
                     /locus_tag="VEA63_07095"
     CDS             211..>1231
                     /locus_tag="VEA63_07095"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HYD83800.1"
                     /translation="MADALALRHSADAEFYRSVLESLGEGVMITDAQWRIQYANGVLH
                     QITGYTPAEFAGLTPHHLFAVDTAMQAECSAQNPIAETEERREMRLRDKSGELRWVYL
                     KTTPFRNAQGEIVGRVTALGCIDRQKELERENEILLAEARENFGHIVGESLALKKVLS
                     QIATVAPTEASVLVLGESGTGKELVARAVHELSPRRARPLVRVNCASIPKELFESEFF
                     GHVRGAFTGAIKDRVGRFELANGGTLFLDEIGEIPLELQGKLLRVLQEGQFERVGDER
                     TRRVEVRIIAATNRDLQAEAKAGRFRLDLYYRLSVFPIELAPLRERLDDIEPLAAHFI
                     RHAARR"
BASE COUNT          232 a          424 c          366 g          209 t
ORIGIN      
        1 aatcgctcac ctaccgagca ctccttagct cgcaacgaaa actcgttatc aacgaacatt
       61 cgttgccatg aaacgagctt tcgtggttgc gccagcgaac gaccgccgtt cgctgccagc
      121 cagtctcctc ccgtttctcc cgccatggtc tcctcacctg tcagcgaacc tgccccgtcc
      181 tccaccgctg ccgcgctgag ctccgacatc gtggccgatg cgctcgcgct acgtcattcc
      241 gcggacgcgg aattctatcg cagcgtcctc gagagcctcg gcgagggcgt gatgatcacc
      301 gacgcacagt ggcgcatcca gtatgcgaac ggcgtcctgc atcaaataac cggatacacg
      361 cccgccgagt tcgccgggct cacgccgcat cacctgttcg cggtcgacac agcgatgcag
      421 gcggaatgca gcgcacaaaa tccgatcgcc gagaccgagg agcggcggga aatgcgcctc
      481 cgcgacaagt cgggcgaact gcgctgggtt tacctgaaga ccacgccgtt tcgcaacgcg
      541 cagggcgaga tcgtcggccg cgtgaccgcg ctcggttgca tcgatcggca aaaagagctc
      601 gagcgcgaaa atgaaatcct tcttgccgaa gcacgcgaaa atttcggcca catcgtcggc
      661 gaaagcctcg cgctgaaaaa agtgctttcg caaatcgcca ccgtcgctcc aaccgaagcc
      721 agcgtgctcg tcctgggcga gtccggcacc ggcaaggaac tcgtcgcgcg cgccgtgcac
      781 gagctgagcc cgcgacgcgc gcgtccgctc gtgcgcgtca attgcgcgtc gattccaaag
      841 gagctgttcg agagcgaatt cttcggccac gtgcgcggcg cgttcaccgg cgcgatcaag
      901 gatcgcgtgg gccgcttcga gctcgccaac ggcggcacgc tctttctcga cgagatcggc
      961 gaaattccgc tcgagctgca gggcaagctg ctgcgggtgt tgcaggaggg ccagttcgag
     1021 cgcgtgggcg acgagcgcac gcggcgtgtg gaggttcgca tcatcgccgc caccaaccgc
     1081 gatcttcagg cggaggcaaa agccggacgt ttccgcctcg acctctacta tcggctcagc
     1141 gtgtttccga tcgaactggc gccgctgcgc gaacgtctcg acgacatcga gccgctcgcc
     1201 gcgcatttca tccgccacgc cgcccgccgc c
//
LOCUS       DASPLT010000676         1742 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_151378, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000676 DASPLT010000000
VERSION     DASPLT010000676.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1742)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1742)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1742
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_151378"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1742)
                     /locus_tag="VEA63_07100"
     CDS             complement(<1..>1742)
                     /locus_tag="VEA63_07100"
                     /inference="COORDINATES: protein motif:HMM:NF014116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate binding domain-containing protein"
                     /protein_id="HYD83801.1"
                     /translation="RLTFWARGANIASSANVIAAVYHAGTGASLLTETVAVTTAWQKF
                     EYEFIAATDVPQAASSLSFVLKEAGTLWLDQVSVAARTASHFPNSSFEAWSGGFPTNW
                     LYTSSPSGVTRTYTQDPGRVGSSSLKIDCTVYPGAPGSTSVADAFQYGPTVTAGSTMR
                     VSFWAKGSNIGNPANALVRVFDTSTWAYNPLSQPINVTGTWQRFEFEFPITQSISSAY
                     HLFWFTLTEVGTLWVDDVQYAVRDASLAANSSFETWSSGFPTSWNRTTSPAGVTVNYA
                     ADTGAFGPTALKIDCTVYPGSPTSTSHADVFQTGQALLSGETYHLSFWAKGSNIGSAA
                     NVRVKVVDASGSYVALDQPLDITADAWKKFTFSFPVTSEVDGSNAKLWFQLDEVGTLW
                     LDDVHYTKESRPTNLVGASGSFESWSGGLPAGWTALSSPDGVSVSYSADTGKVGQGVK
                     LLCSGYPASPTSTSHADLQQRPTAWAKGTTYRLSFWAKGQNIDVANSVRVWMVDTSTW
                     AYIPLSATFVPGATWQRFEFDVPISKNVAQPDALLWITFDEVGTLWLDEVYLTSIPSA
                     PSRYNPVVAPVAGAV"
BASE COUNT          338 a          595 c          496 g          313 t
ORIGIN      
        1 accgcgcccg ccaccggcgc gaccaccgga ttataccgcg acggcgcgga ggggatgctc
       61 gtcagataaa cttcgtcgag ccacagcgtg cccacttcat cgaacgtgat ccagagcagc
      121 gcgtcgggtt gcgccacatt cttcgagatc ggcacgtcga actcgaaccg ctgccacgtc
      181 gcgcccggca cgaaggtcgc cgaaagcgga atatacgccc acgtgctcgt atccaccatc
      241 caaacacgca cgctgttcgc cacgtcgatg ttctgcccct tcgcccagaa cgacagccgg
      301 taggtcgtgc ccttcgccca ggccgtcggc cgctgctgca aatcggcgtg gctggtcgaa
      361 gtcggcgacg ccggatagcc ggagcacaga agcttcacgc cttggccgac tttccccgtg
      421 tcggcggagt agctgacgct gacgccgtcc ggtgacgaga gcgccgtcca cccggcaggc
      481 aatccacccg accagctttc gaacgaacca cttgccccca ccaggttcgt cggccggctc
      541 tccttcgtgt aatgcacgtc gtcgagccag agtgtgccca cctcatcgag ctggaaccac
      601 aacttcgcgt tcgatccatc cacttcgctc gtcaccggaa acgaaaacgt gaatttcttc
      661 cacgcatcgg ccgttatatc gagcggctgg tcgagcgcca catacgagcc tgacgcatca
      721 accactttga cccgcacgtt cgccgcgctg ccgatgttgc tgcctttcgc ccagaacgac
      781 aggtgatacg tctcgccgct caagagcgcc tgtcccgtct ggaacacgtc ggcatgactg
      841 gtcgacgtcg gcgagcccgg ataaaccgtg cagtcgatct tcaacgctgt cggtccgaac
      901 gcgcctgtgt cggccgcgta gtttaccgtc accccggccg gcgacgtcgt tcgattccag
      961 ctcgtcggaa agccgctgct ccacgtttcg aacgaactgt tcgccgcgag cgacgcgtcg
     1021 cgaaccgcat attgcacgtc gtcgacccaa agcgtgccca cctctgtcag cgtgaaccag
     1081 aaaagatgat acgccgacga gatgctttgt gtgatcggaa actcgaactc gaatcgctgc
     1141 caggtgcctg tgacattgat cggctggctc aacggattgt aagcccacgt cgaggtgtcg
     1201 aaaacgcgca ccagcgcgtt cgccggattg ccgatgttgc tgcccttcgc ccagaacgag
     1261 acacgcatcg tcgatcctgc cgtcacggtc ggaccgtatt gaaatgcgtc cgccacgctc
     1321 gttgaaccgg gcgctcccgg ataaaccgtg cagtcgatct tcagcgaact gctaccgaca
     1381 cgtcccgggt cttgggtata ggtgcgcgtc acgcctgaag ggctcgacgt ataaagccag
     1441 ttcgtcggaa atccgccgga ccacgcttcg aaggagctgt tcggaaaatg cgacgccgtg
     1501 cgcgccgcca ccgaaacctg gtcgagccat agcgtgccgg cttccttgag cacaaacgaa
     1561 agcgacgacg ccgcctgtgg cacgtccgtg gcggcgatga actcgtattc gaacttctgc
     1621 cacgccgtcg tgacggccac cgtctcggtc agcagcgacg ctccggtgcc cgcgtgataa
     1681 accgccgcga tcacattcgc cgacgaagcg atattcgcgc cgcgcgccca gaaggtcagc
     1741 cg
//
LOCUS       DASPLT010000677         1151 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_47687, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000677 DASPLT010000000
VERSION     DASPLT010000677.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1151)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1151)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1151
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_47687"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..922)
                     /locus_tag="VEA63_07105"
     CDS             complement(<1..922)
                     /locus_tag="VEA63_07105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HYD83802.1"
                     /translation="MQRRLLGHTGLNVSILSFGASSLGGVFRPTDDAESIRTVHTALD
                     LGINFIDVSPFYGATRAESVLGRALRDVARDRYYLATKVGQYGEGEFDFSAARVTRSL
                     DESCARLGVDYIDVLQCHDIEFASIDQIVGETLPALLALKRAGRIGHIGITGLPLKIF
                     PAVLDRVPDGQVETVLSFCRYTLNDISLEPLLPYFAQKGVGVINASPTGMGLLTPRGV
                     PDWHPAPPALVAGARRAVEYCAQRGGDIVKLAIQFAVSHPGIATTLVGSANSENIRRN
                     IAFANEPLDRELLAEVLDQLQPIHNHNFTRG"
     gene            complement(932..>1151)
                     /locus_tag="VEA63_07110"
     CDS             complement(932..>1151)
                     /locus_tag="VEA63_07110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HYD83803.1"
                     /translation="WSAEQIHPFFDTVLEAFSPARLMFGSDWPVCLVAADYARWHSAV
                     ESLVQPLTRDERDAIMGGTATAFYQLAS"
BASE COUNT          223 a          314 c          424 g          190 t
ORIGIN      
        1 ggccgcgggt gaagttgtga ttgtggatcg gctgcaactg atcgaggact tcggcgagca
       61 gttcgcggtc gagcggttcg ttggcgaagg cgatgttacg gcgaatgttt tccgagttcg
      121 cgctaccgac gagtgtcgtg gcgatgccgg gatgcgaaac ggcgaattgg atcgcgagct
      181 tcacgatgtc gccgccgcgt tgcgcgcagt attccacggc gcgacgcgca ccggcgacga
      241 gcgcgggcgg cgcgggatgc cagtcgggca cgccgcgtgg cgtcaacagc cccatgccgg
      301 tcggcgacgc gttgatgacg ccgacaccct tttgggcaaa atacggcagc aacggctcga
      361 gcgagatgtc gttgagcgtg taacggcaga acgaaagcac ggtctcgacc tggccgtcgg
      421 gaacgcggtc gagcaccgcg ggaaaaattt tgaggggcag gccggtgata ccgatgtgac
      481 cgatgcgacc cgcgcgcttg agcgcgagca gcgcggggag cgtttcaccg acgatctggt
      541 cgatcgaggc gaactcgatg tcgtgacatt ggagcacgtc gatgtagtcg acgccaaggc
      601 gcgcgcaact ttcgtcgagg ctgcgagtca cgcgcgccgc ggagaagtcg aactcgcctt
      661 cgccgtattg gccgaccttg gtggcgagat aatagcggtc acgcgcgacg tcgcggagcg
      721 cgcggccgag cacggattcg gcgcgggtgg cgccgtaaaa aggcgagacg tcgatgaagt
      781 tgataccgag atccaaggcc gtatgcacgg ttcggatact ttccgcgtcg tcggtcggac
      841 ggaaaacgcc gccgagtgac gaggcgccga agctgagaat ggaaacattc aggccggtgt
      901 ggccgaggag gcggcgttgc atgcgggtag attaggaagc gagttgataa aaggccgtcg
      961 cggtgccgcc catgatcgcg tcgcgttcgt cgcgggtgag cggttgcacc aggctttcga
     1021 cggccgagtg ccagcgggcg tagtcggcgg caacgaggca gaccggccag tcagatccga
     1081 acatcagccg cgcgggacta aaggcctcga gcacggtatc gaaaaaggga tggatttgtt
     1141 ccgcgctcca g
//
LOCUS       DASPLT010000678         1031 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_151398, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000678 DASPLT010000000
VERSION     DASPLT010000678.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1031)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1031)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1031
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_151398"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            230..790
                     /locus_tag="VEA63_07115"
     CDS             230..790
                     /locus_tag="VEA63_07115"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HYD83804.1"
                     /translation="MKNTHNVAVFVGSLRKGSFNRQIARALAELAPAELKLEIVEIGH
                     LPLYNQDSDDNPPATFTEFRERIKAADAVLFVTPEYNRSVPGVLKNALDVASRPYGKN
                     AWDKKPGAVVSASIGAIGGFGANHHLRQSLAYLNVPTMAQPEAYIGTVDKLLDADGKL
                     ADDGTRKFLSKFMQAYAAWVKAHAQT"
     gene            829..>1031
                     /locus_tag="VEA63_07120"
     CDS             829..>1031
                     /locus_tag="VEA63_07120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002636867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HYD83805.1"
                     /translation="MRRTKIVATLGPVSSSPDVIRQLIRAGMDVARLNFSHGSHEEHA
                     RQIANLRALSQELDTPVTILQDL"
BASE COUNT          219 a          337 c          272 g          203 t
ORIGIN      
        1 actccctcgc gatgtcggcc acggctttgg gtctcgagcc tctcctcgcc gagcactgat
       61 ttttttcacc cgcggtcggc agaattcgcg aaccatttcg caaattcctc ctttcattgt
      121 atcctcgttc cacccccgcc cgcgcgtcgt cgcggaaatt cccggcggtg cagtttcacc
      181 ccatcacgaa gcgcgcgcgg cttcctcatc aaaccaacaa attcaagaaa tgaaaaacac
      241 ccacaacgtt gccgtcttcg tcggcagcct gagaaaagga tcgttcaacc gtcaaatcgc
      301 tcgcgccctc gccgagctcg cacccgcgga gctgaaactc gaaatcgtcg agatcggcca
      361 tcttccgctc tacaatcagg acagcgacga caatccgcct gccacgttca cggaatttcg
      421 cgaacggatc aaagccgcgg atgccgtgct cttcgtgacg ccggagtata atcgctccgt
      481 tccaggcgtg ttgaaaaacg cgttggatgt cgcatcgcgg ccctacggta aaaacgcgtg
      541 ggacaagaag cccggcgcgg tcgtcagcgc atcgatcggc gccatcggcg gcttcggcgc
      601 caaccaccat ttgcggcaat ccctcgctta cctgaacgtg cctacgatgg cgcagccgga
      661 agcttacatc ggcacggtcg acaaattgct cgacgcggat ggcaaactgg ccgacgacgg
      721 aacccgaaag ttcctgtcga agttcatgca ggcttatgcc gcatgggtga aagctcatgc
      781 tcaaacttga gtaacgtgca cctctcacgc gattcatccg gcggcaccat gcgtcgcaca
      841 aaaattgtgg cgacgctcgg cccggtcagc agttcgccgg atgtcattcg ccaactgatc
      901 cgggcgggca tggatgtcgc gcgcctcaac ttttcgcacg gcagtcacga ggagcatgcg
      961 cggcagattg cgaacttgcg cgccctgtcg caggaactcg atacacccgt gactatcctt
     1021 caggatctgc a
//
LOCUS       DASPLT010000679         1011 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_172544, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000679 DASPLT010000000
VERSION     DASPLT010000679.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1011)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1011)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1011
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_172544"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1011
                     /locus_tag="VEA63_07125"
     CDS             <1..>1011
                     /locus_tag="VEA63_07125"
                     /inference="COORDINATES: protein motif:HMM:NF014895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative PEP-binding protein"
                     /protein_id="HYD83806.1"
                     /translation="RGGRTCHAAIIARELGIPAIVGCGNATAKLKNDESVTVSCAEGD
                     TGNVYRGALEYDIVRHDVASMPPVPVKLALNVGNPDMAFEFRNIPNSGVGLARTEFAI
                     NNMIGVHPKAILDYDTLPAELQAAVRKRAAGYATPRAFYVEKLVEGIATIAAAFWPKP
                     VIVRTSDFKSNEYGKLLGGGLYEPDEENPMLGFRGASRYVSEEFRECFRMECEALKKV
                     REVLGYTNVEIMIPFVRTLNEAQAVIERLAEHGLRRGEHDLRLIMMCEIPSNVVLAER
                     FLEYFDGFSIGSNDLTQLTLGVDRDSALVAQAFDERDPASLAMLATAIKACRKLDKYV
                     GIC"
BASE COUNT          187 a          331 c          314 g          179 t
ORIGIN      
        1 cgcggcgggc gcacctgtca cgcggcgatc atcgcgcgcg agctcggcat cccggccatc
       61 gtcggttgcg gcaacgccac cgcgaaattg aagaacgacg agagtgtcac ggtctcctgc
      121 gccgagggcg acaccggcaa cgtctatcgc ggcgcgctgg agtacgacat cgtgcggcat
      181 gatgtcgcgt cgatgccgcc ggtgcccgtc aagctcgcgc tcaacgtcgg caatcccgac
      241 atggcgttcg agttccgcaa catcccgaac agcggcgtcg gtcttgcgcg taccgagttc
      301 gccatcaaca acatgattgg cgtgcacccg aaggcgatcc ttgattacga cacgctgccc
      361 gccgagttgc aggccgcggt acgcaagcgc gctgccggct atgcgacgcc ccgcgccttc
      421 tacgtcgaaa aactcgtcga gggaatcgcc accatcgcgg ccgccttctg gccgaagccg
      481 gtgatcgtgc gcacctccga cttcaagtcc aacgaatacg gcaagctgct cggcggtggg
      541 ctgtacgagc cggacgagga gaacccgatg ctcggcttcc gcggcgcctc gcgctatgtc
      601 tccgaggagt tccgtgaatg cttccgcatg gaatgcgagg ccttgaagaa ggtgcgcgaa
      661 gtgctcggct ataccaacgt cgagatcatg attccgttcg tgcgcacgct gaacgaggcg
      721 caggcggtca tcgaacggct ggccgaacac ggactcaggc gaggcgaaca cgatctgcgc
      781 ctcatcatga tgtgcgagat cccgtcgaac gtcgtgctcg cggagcggtt cctcgaatac
      841 ttcgacggct tctccatcgg gtcgaacgac ctcactcaac tgacgctcgg ggtggatcgc
      901 gattcggcgc tcgttgccca agctttcgac gagcgcgatc cggcctcgct tgccatgctc
      961 gccaccgcca tcaaagcgtg tcgcaagctc gataagtacg tcgggatttg c
//
LOCUS       DASPLT010000680         4096 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_213263, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000680 DASPLT010000000
VERSION     DASPLT010000680.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 4096)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4096)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4096
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_213263"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..815)
                     /locus_tag="VEA63_07130"
     CDS             complement(<1..815)
                     /locus_tag="VEA63_07130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP cone domain-containing protein"
                     /protein_id="HYD83807.1"
                     /translation="MHTLTPSRHRHFQPFSGVSRKGAQLLDRAIAGDERMVLKRDGTR
                     ARFDQSKVTRAIALAFYEVQTDNAPNPDRDHLLACFGLGSAAFGEVMKISAGVAQMLE
                     LYYREGRHPTIEQVQDAVEKAIAAAGHWEVARAYMLYRARHAERRLAHFADNGMSDYI
                     VMAKYARYRPELGRREIFVEGVERVRDMHLEFFAAKLARQLSVNLPADVAALAGENAE
                     LLARALGGARLDAVIAEAFSQVAAKKVLPSMRSMQFGGPAILKNHSRMFNCSFS"
     gene            1288..2466
                     /locus_tag="VEA63_07135"
     CDS             1288..2466
                     /locus_tag="VEA63_07135"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HYD83808.1"
                     /translation="MRALLAFDKFKDALSAPAACDVAADTLRALHATWELDRCPLTDG
                     GEGFAEILTHAAGGHLRSVRVQGPRGEVVDATFGVVALAKLPPAARTLLALPPQLPSD
                     ATLAIVEMAAASGLALLRKSQRDPWHTTTFGTGELLRAAADAGAAAILLGVGGSATND
                     LGLGALAALGVEFQTAAGETVSPPTPARWPEIARIQKSTSPPLPPIRIACDVTNPLLG
                     DTGCAAVYGPQKGLRHTDRARLEQESARLAGLLCAHCGQADALMDVPGAGAAGGISFG
                     LMTAANAMLLPGFALVSAWLGLDTRIAAADLVLTGEGRFDRSSLTGKGPGAIAARAAA
                     LRREVHVFAGTITDSPPPDVRCHAITPDGLPLETALAATRDLLGAAIRRQFAQPAHAP
                     "
     gene            2456..3151
                     /locus_tag="VEA63_07140"
     CDS             2456..3151
                     /locus_tag="VEA63_07140"
                     /inference="COORDINATES: protein motif:HMM:NF021363.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2062 domain-containing protein"
                     /protein_id="HYD83809.1"
                     /translation="MLPDEHHEEKAHRHGRRRRAKRLLRFVPRRAMLHKYPLIGRFAE
                     FARKRSFLWSFKSEHMRPAFYAGSILSLLPVMGVQLPLALLLSLLLRGNFMVMGGLQF
                     ITNPFTAAPIYYATHQLGAKIIEVSGFGHSIEVVEETPEPHAAEPIVPPHLEPARTVP
                     PADPAEIHWTRRVGTAINALVIGGVVTGALLGLALDLLWKLTWANHHQHPKKRPRRHP
                     PHSHSTPPHPPTE"
     gene            3157..>4096
                     /locus_tag="VEA63_07145"
     CDS             3157..>4096
                     /locus_tag="VEA63_07145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1343 domain-containing protein"
                     /protein_id="HYD83810.1"
                     /translation="MCGPFRLLFSERIFAYVALTLGLLGCSASQPPTRPATKAAAPAA
                     PLFTPPPRTQQTTPLVLLGIDVLEALGFSAVKGKRLGLLTHPAGVNRNGVSTVDVLRR
                     APGTKLVALFGPEHGIYGDEKAEVRIADRRDARTGLPVFSLYGRTRKPTAAMLKGLDA
                     MVIDLQDVGTRSYTYVSAMKLTMEACFEANVEVIVLDRPNPLGGLKADGPLLDAKWTS
                     YVGAFRVPYVHGLTIAELARMAKEAPGVLAIPDAVRARGKLTVIPMRGWRRSMRWPET
                     GLKWVPTSPMIQDFSAVIGYAMVGLGTYFDPRSNFDI"
BASE COUNT          708 a         1521 c         1161 g          706 t
ORIGIN      
        1 gagaagctgc aattaaacat ccggctgtgg ttcttgagaa tcgccggccc gccaaactgc
       61 atcgagcgca tgctcggcag caccttcttc gccgccacct gcgaaaacgc ctccgcgatc
      121 accgcatcca agcgcgcgcc accgagcgcc cgcgccagca gttccgcgtt ctcgccggcc
      181 agcgcagcga cgtctgccgg caagttgacc gacaactgcc gcgccagctt cgccgcgaaa
      241 aattcgagat gcatgtcgcg cacgcgctcg acgccttcga cgaaaatctc gcgccgcccg
      301 agctccggcc gatagcgcgc gtatttcgcc atcacgatat agtcgctcat cccgttgtcc
      361 gcgaagtgcg cgagccgccg ctccgcatga cgcgcgcgat acaacatgta agcccgcgcc
      421 acttcccaat gccccgccgc cgcgatcgct ttctccaccg cgtcctgcac ctgctcgatc
      481 gtcggatgcc gaccctcgcg ataatacaac tccagcatct gcgcgacccc cgccgaaatt
      541 ttcatcacct cgccaaacgc cgcgctgccc aacccgaaac acgccagcaa gtgatcgcga
      601 tccggattcg gcgcgttgtc cgtctgcact tcgtagaacg ccagagcgat cgcccgcgtc
      661 accttcgatt gatcgaaacg cgcgcgcgtc ccatcgcgct tcaacaccat gcgctcgtcc
      721 cccgcgatcg cccgatcaag caactgcgcg ccctttcgcg acaccccgct aaaaggctga
      781 aagtgacggt gacgggaggg cgtgagcgtg tgcatggcga attcgggaaa acggttggaa
      841 cgaagagtga atgagccggc gaccgcgggt tgaaccgccc gcacgccgtg cctcgaaaaa
      901 agcgggtcac cactaccagt cgcgtcgctt cggtgggtca acccctacta attgtggtca
      961 gcgaactatt acctcaaatg atgacgcgca tcttgccagg tccgaaacga gcgccgttcc
     1021 tgcgtctacg tccgcgcttc aaggcctcgc gcacgcagcg cgttagacga cgctttgcga
     1081 gatcaggcgt ccgcgatttc agaaccggcg cttaagcaaa aaatgcgcag cgactcgcgc
     1141 cgcagatctc cacgacacct gagtgcatcg cacgggctcg acaaacaccc ttgcacgtca
     1201 cgcgtcatcg ccgttcgccg cgacggagtc atcgctcgcg acgcaatttc cctcgcctgt
     1261 ctcgccgcga ctcgtctgat ccgaacgatg cgcgcgctcc tcgctttcga caaattcaag
     1321 gatgctctct ccgcccccgc cgcctgcgac gtcgccgccg acacgttgcg cgcgcttcac
     1381 gcgacgtggg agctcgatcg ttgtccgctc accgacggcg gagaagggtt tgcggaaatt
     1441 ctcacgcacg ccgccggcgg tcacctgaga tcggtgcggg tccaaggtcc gcgcggtgaa
     1501 gtcgtcgacg ccacgttcgg cgtcgtcgcg ctcgcgaaac tccctcccgc cgcacgcacg
     1561 ctgctcgcgc ttccgcctca acttccttcc gacgccaccc tcgctatcgt cgaaatggcc
     1621 gccgccagcg gtctcgctct cctgcggaaa tcacagcgcg acccgtggca caccaccacc
     1681 ttcggcaccg gcgaactcct gcgcgccgcc gcggacgccg gcgccgccgc gatcctgctc
     1741 ggcgtcggcg gcagtgcaac caacgatctc gggctcggcg cgctcgccgc gctcggcgtg
     1801 gaatttcaaa ccgccgctgg cgaaaccgtt tctccgccga cgcccgcgcg ttggccggaa
     1861 atcgcgcgta tccaaaaatc aacttcaccg ccgctcccac cgattcgcat cgcctgcgac
     1921 gtcacgaacc cgctgctcgg cgacaccggt tgcgccgccg tttacggccc tcaaaaaggc
     1981 ctccgccaca ccgatcgcgc gcgtctcgaa caagaaagtg cgcgactcgc gggtttgctc
     2041 tgcgctcatt gcggacaagc cgacgcattg atggacgtcc ccggcgccgg cgccgcgggc
     2101 ggaatttcgt tcggcctgat gactgccgcg aacgcgatgc ttctgcccgg tttcgcgctc
     2161 gtttccgctt ggctcgggct cgatacgcgc atcgccgccg ccgatctcgt gctcaccggc
     2221 gaaggtcgtt ttgaccgcag ctcgttgacc ggcaaaggcc cgggcgccat cgccgcgcgc
     2281 gccgcggcac ttcggcgcga ggtgcatgtc ttcgccggaa cgatcaccga ttcaccgccg
     2341 ccggatgtgc gctgtcacgc gatcacccct gacggcctcc cgctggaaac cgcgctcgca
     2401 gccacccgtg acttgctcgg cgcggcgatt cggcgccagt ttgcgcagcc cgctcatgct
     2461 ccctgacgaa catcacgagg aaaaagctca tcgccacggc cgccgtcgcc gcgccaagcg
     2521 tttgctacga ttcgtgccgc ggcgcgcgat gctgcacaaa tatccgctga tcggccgctt
     2581 cgcggagttc gcccgcaaac gctcgttcct ctggtcgttc aaatccgagc acatgcggcc
     2641 cgccttttat gcgggctcga tcctctcgct gctgcccgta atgggagttc aactaccgct
     2701 cgcactcctg ctttcgctgc tgttgcgcgg caacttcatg gtcatgggag gactacagtt
     2761 catcacgaat cccttcaccg ccgcgccgat ctactacgcc acgcatcagc tcggcgcaaa
     2821 gatcatcgaa gtctccggct tcggccacag catcgaagtc gtcgaggaaa cgccagagcc
     2881 ccacgccgcc gagcccatcg tgccgccgca cctcgagccc gcgcgcaccg tgccgccggc
     2941 cgatccggcg gagattcatt ggacccgacg cgtcggcacc gcgatcaacg cgctcgtgat
     3001 cggcggcgtc gtgaccggcg cgttgctcgg cctcgccctc gacctgcttt ggaaactcac
     3061 gtgggcaaac caccaccagc atccaaagaa aagaccacgc cgccatccgc cgcattccca
     3121 ctcgacgcca ccgcacccgc caacagagtg acccgaatgt gcgggccctt tcgccttctt
     3181 ttttccgagc gtattttcgc ttatgttgcc ctgacgcttg gtttgctcgg ttgcagcgcg
     3241 agccagcctc ccacccgccc cgccactaaa gccgccgcac ccgccgcgcc gctgttcacc
     3301 ccgccgccgc gcacgcagca aacgacaccg ctcgtccttc tcggcatcga cgtgctcgag
     3361 gcgctgggtt tctccgcggt caaaggcaaa cgcctcggcc tgctcacaca tcctgccggc
     3421 gtgaaccgca acggcgtcag caccgtcgac gtgctccgcc gcgcgcccgg cacgaaactc
     3481 gttgcgctgt tcggtcccga gcacggcatc tacggcgacg aaaaagccga ggtccgcatc
     3541 gccgatcgcc gcgacgcgcg cacgggcctc ccagtgtttt ccctctacgg gcgaacccgc
     3601 aagcccactg cggcgatgtt gaagggcctc gatgcgatgg tgatcgatct ccaggacgtc
     3661 ggcacgcgca gctacacgta tgtcagcgcg atgaagctga cgatggaagc atgcttcgag
     3721 gccaacgtcg aagtcatcgt cctcgaccgg cccaacccgc tcggcggtct taaagcggac
     3781 gggcctttgc tcgacgcgaa atggacgagc tacgtcggcg cgttccgcgt tccctacgtg
     3841 cacggtctca cgatcgcgga gctcgcgcgc atggcgaagg aagcgcccgg cgtcctcgcg
     3901 attcccgacg cggttcgcgc gcgcggaaag ctcacggtca tcccgatgcg cggctggcgg
     3961 cgcagcatgc gctggccgga aaccggcctg aaatgggtgc cgacctcgcc gatgatccag
     4021 gatttctccg ccgtgatcgg ctacgcgatg gtcgggctcg gcacgtattt cgatccgcgt
     4081 tccaatttcg acatcg
//
LOCUS       DASPLT010000681         1550 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_213295, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000681 DASPLT010000000
VERSION     DASPLT010000681.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1550)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1550)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1550
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_213295"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..279
                     /locus_tag="VEA63_07150"
     CDS             <1..279
                     /locus_tag="VEA63_07150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD83811.1"
                     /translation="KHGGGVLEIEARDMENNVAWRGRWHEGSGYVQRGSSVLLRFRLE
                     GSGSDEIVLTLEPLAPHGLLQVSAQRITPTLLGPVARPLGTYLFHRMP"
     gene            363..998
                     /locus_tag="VEA63_07155"
     CDS             363..998
                     /locus_tag="VEA63_07155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HYD83812.1"
                     /translation="MSNEPVPARPRRFSMIRSFVLADVLTLGNGFCGSGSVLAAMNSL
                     VTGESRWLVVAMALLPIALIFDFADGRVARWRRRSSSLGADLDSLADVVSFGLAPAAL
                     GFAVGLRGAVDVAILLYFVGCGISRLARFNTTADALADDSGKVKYFEGTPIPTSVLIV
                     VLLGVLHFQGRLGADMWLGRWTLGELVLHPLTLVYALSGSAMISTIRVPKI"
     gene            complement(1068..>1550)
                     /locus_tag="VEA63_07160"
     CDS             complement(1068..>1550)
                     /locus_tag="VEA63_07160"
                     /inference="COORDINATES: protein motif:HMM:TIGR00686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein YjdM"
                     /protein_id="HYD83813.1"
                     /translation="RAVSISIRPRYDARAPRKVIRMERASLRRQLLACSPPSSTANFT
                     NPVQPTMSTTPPPCPVCTLEDVLSHSDHWECATCGHEWPKEAAPEAARVVKDAHGTVL
                     AEGDTIALIKDLKLGSGSQVLKAGTKAKNIRLVDGDHEIACKIDGSSMALKACFVKKA
                     "
BASE COUNT          246 a          442 c          537 g          325 t
ORIGIN      
        1 aaacacggag gcggggtgtt ggagatcgag gcgcgcgata tggagaacaa tgtggcttgg
       61 cgcgggcgct ggcacgaggg ctccggctac gtgcaacgtg gatcgagcgt gttgttgcgt
      121 tttcgcctcg aaggctcggg tagcgacgaa atcgttttga cgctcgaacc gctcgcgccg
      181 catggcctcc tgcaagtcag cgcgcaacgg atcacgccga cgttgcttgg gccggtcgcg
      241 cgtccgttgg ggacttatct gttccaccgc atgccgtgag gcccaaggta tcggcgcgcg
      301 gagggagcgg ctaatttacg cggaacgggc agcgagcggc attgaccaag cggcgagcca
      361 ccatgtcgaa cgaacccgtg cctgcccgtc cgcgtcgctt ctcgatgatc cgctcgttcg
      421 tgttggcgga tgtgctgacc ttgggaaatg gattttgcgg cagcggctcg gtgctcgcag
      481 cgatgaactc tctcgtgacc ggcgaatcgc gctggctcgt ggttgcgatg gcgctgctgc
      541 cgatcgcgct catcttcgac tttgccgacg gacgtgttgc acggtggcgg cgccgctcgt
      601 cgtcgctcgg cgccgatctc gattcgctgg cggatgtcgt gtcgttcggg ctagctccgg
      661 cggcgcttgg tttcgcggtg gggctgcgcg gcgcagtcga tgtggcgatc ctgctttatt
      721 tcgtcggttg cggaatcagt cgtctcgcac gtttcaacac caccgcggac gcgctcgccg
      781 acgacagcgg caaggtgaaa tatttcgaag gcacgccgat tccgacgagc gtgttgatcg
      841 tcgtgctgct cggcgtattg cattttcaag gacggctcgg cgccgacatg tggctgggac
      901 gttggacgct cggcgagttg gtgctgcatc cgctcacgtt ggtctacgca ctcagcggaa
      961 gtgcaatgat cagcacgatc cgcgtgccga agatttgatg aaatgcgatt cgagccagcc
     1021 tcgcctccga gatcggcctc gtcagccggg ggcgcgccgc gacgacgtca ggcctttttc
     1081 acgaagcacg ctttaagcgc catcgaactg ccgtcgattt tgcacgcgat ctcgtgatcg
     1141 ccgtcgacga gccggatgtt cttggccttc gtgccggctt tcaggacctg cgaaccgctg
     1201 ccgagcttta gatccttgat caacgcgatg gtatcgccct cggcgaggac ggtgccgtgc
     1261 gcgtctttga cgacccgagc ggcctcgggc gcagcttctt tcggccattc gtggccgcag
     1321 gtggcgcact cccaatgatc ggaatggctg agcacgtctt cgagcgtgca gacagggcaa
     1381 ggcggaggag tcgtggacat cgttggttga accgggttgg tgaaattggc ggtcgaagac
     1441 ggtggtgagc aagcgaggag ttgtcggcgg agcgaagcgc gctccatgcg aatgacttta
     1501 cgcggagcac gtgcgtcgta gcgtgggcgg atgctgatcg ataccgcgcg
//
LOCUS       DASPLT010000682         1708 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_109950, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000682 DASPLT010000000
VERSION     DASPLT010000682.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1708)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1708)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1708
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_109950"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(109..909)
                     /locus_tag="VEA63_07165"
     CDS             complement(109..909)
                     /locus_tag="VEA63_07165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD83814.1"
                     /translation="MSRIEKSSHSWPSERSIEAAPETEWGALIPDDQWEVFMSGVNAM
                     EESGVPFLLAGALALATYTGHWRNTKDVDVIVRECDRERAVAALRGVGFEDYFEQQAY
                     DRSWIFRGFKDGVLFDVIWALPNHRVGVDEAWFAHATPVCLRGRKLRTAPAEEIVRVK
                     LYVMQRERCDWVDVLNVMAAWVERIDWAHLVERMGRDLPLLQGALAIFNWMCPGRAQA
                     LPAWLREQFALPRIEADDQAAMEERRVRLFDSRPWFALHQPVDRPLER"
     gene            1366..>1708
                     /locus_tag="VEA63_07170"
     CDS             1366..>1708
                     /locus_tag="VEA63_07170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007358887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HYD83815.1"
                     /translation="MSSPSVENVVIIGTGCAGLTAAIYTGRANLNPLVLEGSLPGGQL
                     TTTSEVENFPGFPQGVDGFQLMQNLREQATKFGTRFEQALVTAVDFTSQPRKLICGDR
                     TILAKSIIIATG"
BASE COUNT          345 a          650 c          426 g          287 t
ORIGIN      
        1 aaccacgaat ggacacgaac ggacacgaat ggagaaaagc gataaaggcg gcgcggtccg
       61 gatattcgtg tctgttcgtg ttcattcgtg gttaactaaa aacgtgttct accgctccag
      121 cggccgatcc accggctgat gcagcgcgaa ccacggccgg ctatcgaaca gccgcacccg
      181 ccgctcctcc atcgccgcct ggtcgtccgc ctcgatccgc ggcagcgcga attgctcgcg
      241 cagccacgcc ggcaacgcct gcgctcggcc cggacacatc cagttgaaaa tcgccagcgc
      301 cccctgcagc agcggcaaat cgcgtcccat ccgctccacc aaatgcgccc aatcgatccg
      361 ttcgacccac gccgccatca cgttcaacac gtcgacccag tcgcaacgtt cgcgctgcat
      421 cacatacaac ttcacccgca cgatttcctc cgccggcgcc gtgcgaagtt ttcttccgcg
      481 caagcaaacc ggcgtcgcat gtgcaaacca cgcttcgtcg acgcccaccc gatgattcgg
      541 cagcgcccag atcacatcga acagcacgcc gtccttgaac ccgcgaaaaa tccagctccg
      601 gtcatacgcc tgctgctcga aataatcttc gaaacccacg ccacgcagcg ccgcgaccgc
      661 ccgctcgcga tcgcattcac gcacgatcac gtcgacgtcc ttcgtgttgc gccagtgtcc
      721 cgtgtaagtc gccagtgcca gcgcgcccgc cagcaaaaac ggcacgcccg actcctccat
      781 cgcgttcacg ccggacatga acacttccca ctgatcgtcg ggaatgagcg ccccccactc
      841 cgtctcgggc gccgcctcga tcgatcgctc ggacggccag gaatgggatg atttttcaat
      901 gcgggacatg acgatttttc ggatcgcgtt ccgcagacca accatccgcg tgcacgttcc
      961 cgcaactgta gaggggcttt acgccccgac aaagcgccgc aaccaccttc atcctccacc
     1021 gcgccgccac ttcgatacgc cgacaagcgc gaacccgcgc ctcgctcgac gtgcatcccg
     1081 tgaacattgc ttcaaaactc gtcccgctcc tcacggagcg agccacaccg ctgccgtagc
     1141 tctgcgcgtg agcgcaggga ttcacaccgc tgccgtagct ctgtgcgtga gcgcagggac
     1201 tcgcacggtc aacgattcgc acgatcagtc cacagcgcca cccgcccttt cacgcttcac
     1261 cgcaccgcgc acaccaccgc gcagaccgca ccgcgcacgt cgcgccaact cgttaccact
     1321 ttccccttcc caactgtggt atgttgcggc tgaactaaac accgcatgtc ttctccctcc
     1381 gtcgaaaacg tcgtcatcat cggaaccggt tgcgccggct tgaccgccgc catctacacc
     1441 ggccgggcca atctcaaccc gctcgtgctc gagggctcgc tgccgggcgg acagctgacg
     1501 acgacgagtg aggtcgaaaa cttcccggga ttcccccaag gcgtcgacgg tttccagctc
     1561 atgcaaaacc tgcgcgagca ggcgacgaag ttcggcacgc gcttcgaaca agcgctcgtc
     1621 accgccgtcg acttcacctc ccagccgcgc aaactcatct gcggcgatcg caccatcctc
     1681 gcgaaaagca tcatcatcgc caccggcg
//
LOCUS       DASPLT010000683         1250 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_233211, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000683 DASPLT010000000
VERSION     DASPLT010000683.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1250)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1250)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1250
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_233211"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..437
                     /locus_tag="VEA63_07175"
     CDS             <1..437
                     /locus_tag="VEA63_07175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012377323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ThuA domain-containing protein"
                     /protein_id="HYD83816.1"
                     /translation="DSPGKAGHPPRSEFTLVTRTSDHPIMQGLPAEWRQASDEIYSQL
                     RGPAKNVTVLATAYADKAAHPTASGEHEPMLMTISYGKGRVFHTTLGHVGPKETPPYP
                     SLSSVGFIVTLQRGAEWAATGKVTQPIPTDFPTAEKATTRTN"
     gene            complement(810..>1250)
                     /locus_tag="VEA63_07180"
     CDS             complement(810..>1250)
                     /locus_tag="VEA63_07180"
                     /inference="COORDINATES: protein motif:HMM:NF016060.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HYD83817.1"
                     /translation="MKKMRVTLKRPWPEWGRWFAVATLFTAAGLALLKLFVDGLQWPY
                     PVATLVQSELCNLLRFFVNDRWVFGRRRPTWKRLVQYHLANAMGFAVWWLGANALKAM
                     GMNYLLASVVAMLGSFGVSWLTNFKWIWRKRPPPSCETATDHAR"
BASE COUNT          261 a          461 c          311 g          217 t
ORIGIN      
        1 acgactcgcc cggcaaagcc ggccacccgc ctcgctccga attcacgctc gtcacccgca
       61 cctccgatca cccgatcatg caaggcctgc ccgccgaatg gcgccaggcc agcgacgaaa
      121 tctacagcca gcttcgcggc ccggcgaaaa acgtcaccgt tctcgcgacc gcttacgccg
      181 acaaagctgc gcatcccacc gccagcggcg aacacgagcc gatgctcatg accatttcct
      241 acggcaaagg tcgcgtgttt cacaccaccc tcggccacgt cggcccgaag gaaacgcccc
      301 cttatccatc gctttcgagc gttggcttca tcgtcacctt gcaacgcggc gccgagtggg
      361 ccgccaccgg caaagtcacg cagcccatcc caaccgattt ccccaccgcc gaaaaagcga
      421 cgacgcgcac aaactgagcc cgaacaattc atttgaacca cgaatggaca cgaatgaaac
      481 cgagttcgaa tgcgtcgtct tttcggaaca ccaaagggtg atgtcacgat tggccctgaa
      541 agctttcctt cggccactct gaattcgtgt tcattcgtgt ccattcgtgg tttctcttct
      601 gcatgagtcc ggctaagttc tcacggtggg agggctttac gccccgacaa acgcctcgcg
      661 cgctcctccg cgttcactca cgcatgacac cactgcagcg tgcgaacgcc gtgcccagaa
      721 actgaatacg ccgcgcacgg ctccgttgtc atccgcgcta tccggcccta tccccgcgca
      781 cgcggatccg cgctcacgcc acgaccgcct caccgcgcat gatccgtcgc cgtctcgcac
      841 gacggcggcg gtcgctttcg ccagatccat ttgaagttcg tcaaccagct cacgccgaac
      901 gaccccagca tcgcgacgac cgacgccaga agatagttca ttcccattgc cttcagcgca
      961 ttcgcgccca accaccacac cgcgaaaccc attgcgttcg cgaggtggta ttgcacgagg
     1021 cgtttccaag tcggccggcg ccgcccgaac acccagcgat cgttcacgaa gaaacgcagt
     1081 aaattgcaca gttccgactg cacgagcgtc gcgaccggat acggccattg cagcccgtcg
     1141 acgaacaact tgagcaacgc cagccccgcc gcggtgaaca gtgtcgccac cgcgaaccag
     1201 cgcccccatt cgggccaggg tcgtttgagg gtcacgcgca ttttcttcat
//
LOCUS       DASPLT010000684         3231 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_131566, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000684 DASPLT010000000
VERSION     DASPLT010000684.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 3231)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3231)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3231
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_131566"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..282
                     /locus_tag="VEA63_07185"
     CDS             <1..282
                     /locus_tag="VEA63_07185"
                     /inference="COORDINATES: protein motif:HMM:NF024985.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4139 domain-containing protein"
                     /protein_id="HYD83818.1"
                     /translation="GVVSKGKRITYDITLTVENHKRTAETIVVQDQIPVSRHEKIIVK
                     QLAPDERQLKPDTEGVIKWGLELKPGEKRDLPLKFSIEHPADLAVSGLE"
     gene            461..970
                     /locus_tag="VEA63_07190"
     CDS             461..970
                     /locus_tag="VEA63_07190"
                     /inference="COORDINATES: protein motif:HMM:NF016301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="paraquat-inducible protein A"
                     /protein_id="HYD83819.1"
                     /translation="MSSSRAPAPSPASAQQRSVPWLLALAFLLFASGVLLPFFHVTKF
                     WMFDDAVSVVGGIYTLLLEGEYFLFTVLTLFTLVFPCVKLGLLAIIWTEREHNLTRVR
                     RLHHWVEHLGKWSMLDVFVVAILIVAMKSAAVAELHIGLGLYLFTFSIIATQLASGFV
                     ARLLAVLSR"
     gene            complement(1223..1882)
                     /locus_tag="VEA63_07195"
     CDS             complement(1223..1882)
                     /locus_tag="VEA63_07195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD83820.1"
                     /translation="MKQNTTYKTLIAAAALSLGLASTALAQDANVAVPNPADEAPVGG
                     LLGSRYTQVEYNYIDLDGFGPSHADGFGVTLNQPLQPNVDVFASYDWAEAEFAGSKFR
                     VQDAEVGATIYSGLEWGKPFATAAVGWQWQKAGVFKDDSFAFKVGVGSEFQVAPAIVV
                     TPFVNFVRATGFNANEFELGAKATYRINGDWSVTARAQYEAVEHSDNSAEYSLGVNYH
                     F"
     gene            2071..2589
                     /locus_tag="VEA63_07200"
     CDS             2071..2589
                     /locus_tag="VEA63_07200"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HYD83821.1"
                     /translation="MKSRTLHLLALVIFSTLFAAFARADSLKVGDAAPVVAATTDTGD
                     SLDLGDVYKKHNYTLVWFYPKALTGGCTSQGCSLRDAAADLAKHDVAVIGVSVDPVEK
                     QKEFKEANHFPFPLLADTDRAVMKAFGQGDSGMAKREAYLIKDGKIVYKDQGVTTKQA
                     ENVLNYLASQKS"
     gene            complement(2843..>3231)
                     /locus_tag="VEA63_07205"
     CDS             complement(2843..>3231)
                     /locus_tag="VEA63_07205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009510625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD83822.1"
                     /translation="GGMATEIVAVTPEGIVVAKQVLGEPLPQGDDMSASLPAGLIEIP
                     SRFLRANRDHPRLLFHGGAAWLVADLHARNFVRCADGALRVIDLVAAPWPVETAARHP
                     LIGDWLERVRENPAAGLLRVSADEEL"
BASE COUNT          570 a         1192 c          876 g          593 t
ORIGIN      
        1 ggcgtcgtct cgaagggcaa acgtatcacc tacgacatta cgctcaccgt cgagaaccac
       61 aaacgcacgg ccgagacgat cgtcgtgcag gatcagatcc ccgtttcgcg tcacgagaaa
      121 atcatcgtga agcagctcgc gccggacgag cgccagctga aacccgacac cgaaggcgtg
      181 atcaaatggg gcctcgagtt gaaacccggc gagaaacgcg atctcccgct caaatttagc
      241 atcgagcacc ccgccgacct cgccgtctcc ggcctcgagt aacttacttt gtagggcggg
      301 gtctcccgac cccgccgctc cgccgcgcgc caaccgcgcc gagcccatcc cgtcctcatc
      361 ggcgcggcac cgccctacag tccggctctc tggatcgcac cgcactctcc cgccactttt
      421 ccttttgccg ccccctcgcg ctcctgccac cgcttcacgc atgtcatctt cccgcgctcc
      481 cgcgccgtcg cccgcctcgg cccagcagcg cagcgtccct tggctgctcg cgctcgcttt
      541 tcttctcttc gccagcggcg tgctcctgcc gttttttcac gtcacgaaat tctggatgtt
      601 cgacgacgcg gtctccgttg tcggcgggat ctacacgctc ctgctcgaag gagaatattt
      661 tctcttcacc gtcctcaccc tgttcacgct ggtgtttccc tgcgtcaaac tcggcctgct
      721 cgcgatcatc tggaccgagc gcgaacacaa cctcacgcgc gtgcgccgac tgcatcactg
      781 ggtcgaacac ctcggcaaat ggtcgatgct cgatgtattc gtcgtcgcga tactgatcgt
      841 cgccatgaag tccgcggccg tcgccgaact ccatatcggc cttggcctgt atctcttcac
      901 cttttccatc atcgccaccc agctcgcgtc cggcttcgtc gcccgcctcc tcgccgtcct
      961 ttcaaggtag ggcggggcat ccctcacccg cccactcagc ccggcgtagc cgcacccctg
     1021 gcgtagccgc accggttacg gtgcaggccc cgacgcgccc cgcacaatcc gcccgcgcga
     1081 aagccacggc gctcatcacg ccgtggctcg aatccgaaat cgcggcgccg gctggtgcgg
     1141 cgccgcgact cggctaagca acgctatcgg cgaagcgaaa aaggcttgct ggctcctctc
     1201 gctccgcctg gcagcggctc actcagaagt gatagttcac gccgagcgaa tactccgccg
     1261 agttgtcgct gtgctccacg gcctcgtatt gcgcgcgggc cgtcacgctc cagtctccat
     1321 taatccgata cgtcgccttc gcgccgagct cgaattcgtt cgcgttgaaa ccggtcgcgc
     1381 ggacgaagtt cacgaacggc gtgacgacga tcgccggcgc cacttggaat tcgctgccga
     1441 cgcccacctt gaaggcaaac gaatcgtcct tgaaaacgcc cgccttctgc cactgccagc
     1501 ccaccgccgc cgtcgcgaac ggcttgcccc actcgagccc cgagtagatc gtcgcgccca
     1561 cctcggcgtc ctgcacgcgg aacttgcttc ccgcgaattc tgcctcggcc cagtcgtagc
     1621 tggcgaacac gtcgacgttc ggctgcagcg gctggttcag cgtcacgccg aaaccgtccg
     1681 catggctcgg gccgaagcca tcgagatcga tgtagttata ctcgacctgc gtgtaacgcg
     1741 agccgagcag ccctccgacc ggcgcctcgt ccgcgggatt tggcaccgcg acattggcgt
     1801 cctgcgccaa cgccgtggag gcgagaccga gcgacaacgc tgcggccgca attaaagttt
     1861 tatacgtagt gttctgtttc attgtgtagt cttggttctt cccgcggacc gtggcttgcg
     1921 cccgtattcg cggggtggat atgaccgcga accgcgcaaa agttcctcat ggaaaattta
     1981 cttcggtcga aaatttcgct ggaacaatcg cgccgcatga caccaacccc gcttgcgaaa
     2041 caatggcgct gatggagggt atcagttgtc atgaaatccc gcaccctcca cctccttgcc
     2101 ctcgtgatct tctccaccct cttcgccgcc ttcgcacgcg ccgactccct caaagtcggc
     2161 gacgccgctc ccgtcgtcgc cgccaccacc gacactggtg actccctcga tctcggagac
     2221 gtgtataaga agcacaacta cacgctcgtc tggttttacc ccaaggcgct caccggcggt
     2281 tgcaccagcc agggctgttc gctccgcgac gccgccgccg acctcgccaa gcacgacgtc
     2341 gcggtcatcg gcgtgagcgt cgacccggtc gaaaagcaaa aggagttcaa ggaagccaac
     2401 cattttccct tcccgctgct cgccgacacc gaccgcgccg tcatgaaggc cttcggccag
     2461 ggcgattccg gcatggcgaa gcgcgaggcc tatctcatca aggacggcaa gatcgtctac
     2521 aaggaccagg gcgtcaccac gaagcaggcc gaaaacgtcc tcaactacct cgcgtcacag
     2581 aaaagctgat acgatattcg tgcggctcgc tacgtgagca acggaacggc ccgctctccg
     2641 agcgggcctt ttttttgcct tctgccggac cgatcttccg ctgcgacaaa gcccaacggc
     2701 tccgtgccgg cccgctttgt aggttgcgcg ctcgcgcgca acgtggcgcg caagcgcacc
     2761 acccaccttt ctcttcttcg aagcgatatc gggaaatccc gctcgcaggc gcggaggccg
     2821 cacggcgtcg ctcgcctgac gcctacaact cctcgtccgc ggagacccgc aacaatccgg
     2881 ccgccggatt ctcccgcacc cgctccagcc aatcgccgat gagcgggtgc cgcgcggccg
     2941 tttccacggg ccacggcgcc gccaccagat cgatcacgcg cagcgcgccg tcggcgcaac
     3001 gcacgaaatt gcgcgcgtgc aaatccgcca ccagccacgc cgcgccgccg tgaaacaaca
     3061 accgcggatg gtcgcgattc gcgcgcagga agcgcgacgg tatctcgatc aacccggccg
     3121 gcagcgatgc cgacatgtcg tcgccctgcg gcagtggttc gccgagcact tgtttcgcaa
     3181 ccacgatgcc ctccggcgtc accgccacga tctcggtcgc cattccgccg a
//
LOCUS       DASPLT010000685         1868 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_131567, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000685 DASPLT010000000
VERSION     DASPLT010000685.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1868)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1868)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1868
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_131567"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            129..1628
                     /locus_tag="VEA63_07210"
     CDS             129..1628
                     /locus_tag="VEA63_07210"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HYD83823.1"
                     /translation="MLPAPSASFTLTPFRRLRSYLAFVAAALTPVLLGAETLLTLEDA
                     IRFALDKNPQIKVDAFSRSIARADLLAARGQFDPALTFRRSYSEDGAPASSNPLVAQL
                     IQTDDYSLALEGTTPWGMNYSLGGSATNQRGGFLGYQDNYATFGGITVTQPLLRGFGF
                     GPNLLGVRIAKADRGIADWLFRQTVIDTVTQVAIAYSDVAYAQQQLRIAQRSRELAAG
                     LLAENEKRFSVGSMSESDVTQARARTATRDEAILFSEQALRDAINYLRQLMGETAFSI
                     EPENLLVNPPEIPDDLDIRPAEDLQLAYEQRPDFQAAKLGIVKRRATHLAERNQLLPQ
                     VDFVGSYGYSGLDRDFGVSRRMVANNDNRSYSAGVVVSIPLTFAEGRGRARSARLRLH
                     QAEADLLLLEQEIAVGIARAAGQIETTRRRVAANRAAQDLAREALDAELKKLRAGTSN
                     TFFVLQQQEEVSRAENNLYRALADQRRAVALYDRELGRTLEARQITVEQ"
BASE COUNT          356 a          739 c          466 g          307 t
ORIGIN      
        1 cgaatgattc cgctgccgga cacgccaacc aaccgctcgc cctgcgcata atttttagtc
       61 actaatctcg agaagcttac atatctttat ccgccgtggc acgccccctg caaaacctgg
      121 cccatccgat gctccccgct ccttccgcca gcttcacgct cacacctttt cgccgtctgc
      181 gatcctatct cgcctttgtc gccgccgcgc tcactcccgt tctcctcggc gccgaaacgc
      241 tcctcacgct cgaggacgcc atccgcttcg ccctcgacaa aaatccgcag atcaaagtcg
      301 acgcgttcag ccgctccatc gcgcgcgccg acctcctcgc cgcgcgcggc cagttcgatc
      361 ccgctctcac gtttcgccgc agctacagcg aagacggcgc gcctgcgtcc tccaatccgc
      421 tcgtcgcgca actcatccaa accgacgact acagcctcgc cctcgaaggc accacgccgt
      481 ggggcatgaa ctacagcctc ggcggcagcg cgacgaatca acgcggcggc ttcctcggtt
      541 atcaggataa ctacgccacg ttcggcggca tcaccgttac acaacctctc ctccgcggct
      601 tcggcttcgg tccgaacctc ctcggcgtcc gcatcgccaa agccgaccgc ggcatcgccg
      661 actggctgtt tcgccagacc gtgatcgaca ccgtcaccca agtcgcgatc gcctacagcg
      721 acgtcgccta cgcgcaacaa cagctccgca tcgcccaacg ttcacgcgag ctcgccgccg
      781 gccttctcgc tgaaaacgaa aaacgtttca gcgtcggcag catgtccgaa agcgacgtca
      841 cgcaagcgcg cgcccgcacc gccacccgcg acgaagccat cctcttttcc gagcaggcgc
      901 tgcgcgacgc catcaactac ctccgccagc tcatgggcga aaccgcattt tcgatcgagc
      961 ccgagaacct cctggtcaac ccgccggaaa ttcccgacga cctcgatatt cgccccgccg
     1021 aggaccttca actcgcctac gaacagcgcc ccgactttca ggccgcgaaa ctcggcatcg
     1081 tcaaacgccg cgccacccac ctcgccgagc gcaaccagct tttgccccag gtcgatttcg
     1141 tcggcagcta cggttacagc ggactcgatc gcgatttcgg cgtgagtcgt cgcatggtgg
     1201 ccaacaacga caaccgttcc tactccgcgg gcgtcgtcgt cagcatcccg ctcacctttg
     1261 ccgaaggccg cggccgcgct cgctccgcgc gcctccgcct tcatcaagcc gaagccgact
     1321 tgctgcttct cgagcaggaa atcgccgtcg gaatcgcccg cgccgccggc cagatcgaaa
     1381 cgactcgccg tcgcgtcgcc gccaaccgcg ccgcacaaga cctcgctcgc gaagcgctcg
     1441 acgccgagtt gaagaaactt cgcgccggca cgagcaatac gttcttcgtt ctccagcagc
     1501 aggaggaagt ctcccgcgcg gaaaacaatc tctaccgcgc gctcgccgac caacgccgcg
     1561 ccgtcgccct ctacgaccgc gaactcggcc gcaccctcga agcccgccaa atcaccgtcg
     1621 agcagtagcg cctaaaagta gcggcaggcc tccgtgcctg cctgcccgtc cgtcccacgt
     1681 gacccgatgc agtcgtcgct cacgaaccca ccgccaccgc cgccggtcaa acggcgcgcc
     1741 ggcgccggta ggggcgcctc tccgaggcgt ccgccgaaaa cggaagagcc gggacttcta
     1801 aaaatatccg ccgaacacct cgctgccgca gttaaagcaa agttgcagcg taacatcgcg
     1861 cttctttg
//
LOCUS       DASPLT010000686         1096 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_151470, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000686 DASPLT010000000
VERSION     DASPLT010000686.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1096)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1096)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1096
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_151470"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            18..>1096
                     /locus_tag="VEA63_07215"
     CDS             18..>1096
                     /locus_tag="VEA63_07215"
                     /inference="COORDINATES: protein motif:HMM:NF021534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2264 domain-containing protein"
                     /protein_id="HYD83824.1"
                     /translation="MDHALDEFLKNYRDCFARDGGPIPWGRSLAYRFGAIAPFGWAAW
                     NGTGSLPPGELRRISSGALRYFWERGAMSADGLLHPGYHGPNGVAAEFYIGAGTPYFA
                     IQGLIALMIPEDHPFWNDTELPMPADQGEGVAVLTGPEMVVRIKPDGDARLYPVSQRF
                     SRAHQHWQRGVKYQQHAYSSYLGWAALGEGSDLGAGRTGVSTDGKTWQYRDHARAISL
                     ADDHIASAYGVEIDTTYRPAIDDRYEVITHTLLGAAGEVHVFWHRSPQSLHLHLGGYG
                     ISAPDARTIVSTAPAGGIEIETPDYFSVMRVVSAPEGNLAHEVLEPREGWRHAHLFGG
                     IGTFPVWRSQNVVPANTPVIIYVEGG"
BASE COUNT          194 a          321 c          372 g          209 t
ORIGIN      
        1 agcaattcgg cgaacgaatg gatcacgccc tcgacgagtt tctgaaaaac taccgcgact
       61 gtttcgcccg cgacggcgga ccgattcctt ggggtcgctc gctcgcgtat cgtttcggcg
      121 cgatcgcccc gttcggctgg gcggcttgga acggcaccgg atcgctgccg ccgggagaac
      181 tgcggcggat ttcgtccgga gcactgcgtt atttttggga gcgcggcgcg atgagcgcgg
      241 atggattgct gcatccgggg tatcacgggc cgaatggggt cgccgccgag ttctacatcg
      301 gcgcgggcac gccatatttc gcgatccaag gtttgatcgc gctgatgatt ccggaggatc
      361 atccgttctg gaacgacacg gagttgccga tgccagccga tcaaggcgaa ggtgtcgcgg
      421 tgctgaccgg cccggaaatg gtcgtgcgca tcaaaccaga cggcgacgcg cggctctatc
      481 cggtgagcca gcgcttttcc cgtgcgcacc agcattggca gcgcggtgtg aagtatcagc
      541 agcatgcgta ttccagttat ctcggttggg cggcgctggg ggagggcagc gatctcgggg
      601 cgggtcgcac tggcgtgtcg accgacggga aaacgtggca gtatcgtgat cacgcgcgtg
      661 cgatttcgct ggccgacgat catatcgcga gcgcttacgg cgtggagatc gacacgacct
      721 accggccggc gatcgacgat cgctatgagg tgataacgca cacgttgctc ggagcggcgg
      781 gcgaggttca cgtcttctgg caccgcagcc cgcagtcgct tcatctgcat ctcggcggct
      841 atggcatcag cgcgccggat gcgcgcacca ttgtgagcac cgcaccagca ggcgggatcg
      901 agatcgaaac gccggactat ttctccgtga tgcgcgtggt cagcgcgccc gaaggcaatc
      961 ttgctcacga agtgctggag cctcgggaag gctggagaca cgcgcatctt ttcggcggca
     1021 tcggcacgtt tccagtctgg cgcagccaaa acgtcgtgcc ggccaacacg cccgtgatta
     1081 tctatgtcga gggagg
//
LOCUS       DASPLT010000687         1108 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_213320, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000687 DASPLT010000000
VERSION     DASPLT010000687.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1108)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1108)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1108
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_213320"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..524)
                     /locus_tag="VEA63_07220"
     CDS             complement(<1..524)
                     /locus_tag="VEA63_07220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD83825.1"
                     /translation="MATRASPLASGPHAPLSRLIRGLTFRQVMVLTTGCFILVAAGVV
                     TVAMVSRNYSKQGMRQTETLTGNFLPGLVTLARLQDSTLNLKSLTFQLALARDEAAIA
                     EQKRAFADYTTQVDRGVRQLTTMAQDGRIVELTAGLDAGVQSFLSGTEKFQAQLGAGE
                     FEQAMATLDLVIVPA"
     gene            complement(889..>1108)
                     /locus_tag="VEA63_07225"
     CDS             complement(889..>1108)
                     /locus_tag="VEA63_07225"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HYD83826.1"
                     /translation="PAVLLPMSDFNGEKKLWKLFLENNAGKEIIVYCRSGNRSGQVAQ
                     KLADDGKTVANAGAFKDWVAADLPVRQP"
BASE COUNT          195 a          304 c          402 g          207 t
ORIGIN      
        1 gccgggacga tgacgaggtc gagggtggcc atcgcttgtt caaactcgcc ggcgcccagt
       61 tgcgcttgga atttctcggt gccgctgagg aagctttgga cgccggcgtc caagccggcg
      121 gtcaattcca cgatgcgtcc atcctgcgcc atcgtcgtga gctggcggac gccacggtca
      181 acttgcgtgg tgtagtcggc gaaggcgcgc ttttgttcgg cgatcgccgc ttcgtcacgg
      241 gcaagggcga gctggaatgt gaggcttttt agattgagcg tggagtcctg aaggcgcgcg
      301 agcgtgacaa gacccggtaa aaagttgccg gtgagcgttt cggtctgacg catgccctgc
      361 ttggagtaat tgcgcgagac catcgcgacg gtcacgacgc cggccgcgac caggatgaag
      421 cagcccgtag tcaacaccat gacctgtcga aaagtcaggc cacggatcag cctggagagc
      481 ggcgcgtggg gacctgacgc gagtggcgaa gcgcgggtgg ccacgggcgg gtttgcagcg
      541 tttgagggcg aagcggacat gtgtgcggcg tgcgagatag cggtagaagg aagggccttc
      601 cgtcgatcac gtccggacgg cagcgtccgg gccgctcgcg gcataggtgt gtgcaaacct
      661 acatcggttg gcgcgcgctt cccatggagt cacaatttcg tcacaatgcc gacgtgttgc
      721 gcggcggctc acggacgcac gacgcaaagg agatcgggca cgcttgggag gatgaagtgc
      781 ggaccgatct ggcgcgtcgt atttgcgcgc ccgccggggt tgggcgcgcc acgatgcggc
      841 gccgagatgg actggggcgc cttctacgag gcgcgattca aaccgcgatc agggctggcg
      901 aacagggagg tcggcggcga cccagtcttt gaacgcgccg gcgttggcga cggttttacc
      961 gtcgtcggcg agtttttgcg cgacttggcc ggagcgatta ccggagcggc agtagacgat
     1021 gatctccttg ccggcgttgt tctcgagaaa gagtttccag agctttttct cgccgttgaa
     1081 atcgctcatc gggagcagca cggcgggc
//
LOCUS       DASPLT010000688         1702 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_31979, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000688 DASPLT010000000
VERSION     DASPLT010000688.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1702)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1702)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1702
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_31979"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1553
                     /locus_tag="VEA63_07230"
     CDS             <1..1553
                     /locus_tag="VEA63_07230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HYD83827.1"
                     /translation="RISEHTQLTVKLVAAEHWIFREPLLILDPSVDANTRDPQLAPGI
                     SKRGLNGIQPWSHVGTHTADLFTLLTSNLTENISVRFAANGRYLFEDSTQEFLSTPNL
                     TNRYNPMTGELTQDYTWALDPAIGTYVATYSPFYNPAAIPVRGDKQATRRKTVSVQTD
                     AVARYRFGSVTSQTVAGVALSRRTNYGRGVSGALPGIDLSQPNRRAVPAWSSEWAFYN
                     DSHFTNWQAYVNERLGFFADRLQLTAGVLHYDTHTYARNHLLNAAPGVLDDHKAMWMA
                     SVLIKPRPNLAFYYNHSTNASPVIANNVPLWRDGVQDEIGFKSEFFNQRLSFNAAYFE
                     IAQTNVTVPNPDRQTDPTAPETLVSDSGNHGIEFELVGGVTPNLSVIATFTHLKMRDN
                     LGRPVRAVADRNAALLLNYRFRETRFDGLALTAGVSYSGRRAGDTPINFTPLNVVGRT
                     SFFLKPYYVTTLGLSYRWNENYLFRFNIDNVLDDKGYIQQAGARVSGTGITTAPGINV
                     KLTATVTF"
BASE COUNT          337 a          660 c          397 g          308 t
ORIGIN      
        1 atcgcatttc cgagcacacg cagctcaccg tgaaactggt cgccgccgaa cactggattt
       61 tccgcgagcc gctcctgatt ctcgatccgt ccgtcgatgc caacacccgc gatccgcaac
      121 tcgcgcccgg catctccaag cgcggcctca acggcattca accctggagc catgtcggca
      181 cgcacaccgc cgatctcttc acgctcctca ccagtaatct caccgaaaac atcagcgtcc
      241 gcttcgccgc caacggccgt tatctgttcg aagattccac gcaggagttt ctctccacgc
      301 ccaatctcac caatcgctac aatccgatga cgggcgagct cacgcaggat tacacctggg
      361 cactcgatcc tgcgatcggc acctatgtcg cgacctattc tccgttctac aatcctgcgg
      421 ccattcccgt ccgcggcgac aagcaggcca ctcgtcgcaa aaccgtcagc gttcaaaccg
      481 atgccgtcgc tcgttaccgc ttcggctccg tcacctcgca aaccgtcgcg ggcgtcgcct
      541 tgtcgcgccg caccaactac ggccgcggcg tctccggcgc gctccccggc atcgatctca
      601 gccagccgaa tcgccgcgca gttcccgcat ggtcgtccga atgggcgttc tacaacgaca
      661 gccacttcac caactggcag gcctacgtga acgagcgcct cggcttcttc gccgaccgcc
      721 tccagctcac cgccggcgtt ctccactacg atacgcacac ctacgcccgc aaccacctgc
      781 tgaacgccgc tcccggcgtc ctcgacgatc acaaggccat gtggatggcc agcgtgctga
      841 tcaaaccgcg ccccaacctc gcgttctact acaatcattc caccaacgcc tcgcccgtca
      901 tcgccaacaa cgtccctctc tggcgcgacg gcgtgcagga cgagatcggt ttcaaaagcg
      961 agttcttcaa ccaacgcctc tccttcaacg ccgcgtattt cgaaatcgcg cagaccaacg
     1021 tcaccgtgcc caaccccgat cggcaaaccg atcccacggc gcccgagacg ctcgtctccg
     1081 actcgggcaa ccacggcatc gaattcgaac tcgtcggcgg cgtcactccg aacctctcgg
     1141 tcatcgccac gttcacgcac ttgaaaatgc gcgacaacct cggccgcccc gtgcgcgccg
     1201 tcgccgaccg caacgccgcc ctcctgctca attaccgctt ccgcgaaacc cgcttcgacg
     1261 gtctcgcgct caccgccggc gtcagctaca gcggccgccg cgccggcgac acgccgatca
     1321 atttcacgcc gctcaacgtc gtcggtcgca ccagtttttt cctaaaaccg tattacgtca
     1381 ccacgctcgg cctctcgtat cgctggaacg aaaactatct cttccgtttc aacatcgaca
     1441 acgttctcga cgacaaaggc tacatccagc aagccggcgc ccgcgtctcc ggcaccggca
     1501 tcacgaccgc ccccggcatc aacgtaaaac tcaccgccac ggtgaccttc taagtaggct
     1561 tctagcccgc attcatcacc cgcagatgcc gggtgcgcgc agtctgttgt agcgaaaggg
     1621 gtgaattttc cggttaagta gggcggcgct tcagcccggc ccctgacgca acgacgtcaa
     1681 agaaaacagc tctctctctc ca
//
LOCUS       DASPLT010000689         1357 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_32002, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000689 DASPLT010000000
VERSION     DASPLT010000689.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1357)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1357)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1357
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_32002"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..326)
                     /locus_tag="VEA63_07235"
     CDS             complement(<1..326)
                     /locus_tag="VEA63_07235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HYD83828.1"
                     /translation="MKKTIQTLIALAAFSATALIAQAQPAPKILVVDMAKLYDGHYKT
                     EEQNAKLRGDEQKAQEELDKLNKEGNDLVEQYKELVDQSNNPAATAEAKSKAQDEAQR
                     MLEQIQR"
     gene            complement(371..>1357)
                     /locus_tag="VEA63_07240"
     CDS             complement(371..>1357)
                     /locus_tag="VEA63_07240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HYD83829.1"
                     /translation="LERATVFAEISQGNFDLFNRRSFFQGDGQKFRLKLSLGSLSSEV
                     SMSFEEPWLFEQQLALGTNLFRTTSEYNSTFYTEIDTGGEVYLRKRLFGLFEGRLSYS
                     YTVISIEDVAPNASPILRSLEGDNPISQIGFQLLRDTRDKIINTTSGNRLEVNTAVAG
                     GPLGGQDNYYSLELRGSQFFPVFETQAQVLSLIGRAGVINNFGDSATVAPYKRWYLGG
                     PTTLRGYEFREVAPRDEFNEVIGGKAYAFFSAEYSFDIVSPIRFAIFYDAGFVNAGAY
                     DFSPADFTDNFGFGVRLFVAGSPLSLDFGIPLTSDPRSRDGNQFNFSFGTRY"
BASE COUNT          276 a          366 c          429 g          286 t
ORIGIN      
        1 cgctggatct gctcaagcat gcgctgcgct tcgtcctgcg ccttcgactt cgcctcggcg
       61 gtggcggccg ggttgttcga ctgatcgacg agctctttgt attgctcgac gaggtcgttg
      121 ccttctttgt tgagcttatc gagttcctct tgggccttct gctcgtcgcc ccggagcttc
      181 gcgttttgct cctcggtctt gtagtggccg tcgtaaagct tggccatgtc gacgacgagg
      241 atcttgggcg ccggctgggc ttgggcgatc agcgcggtgg cgctaaacgc tgccagggcg
      301 atcaaggttt ggatggtttt tttcatggat gggtgatgat tggggaagac gaatgttcag
      361 caaaacgcaa tcagtagcgc gtgccaaagg aaaagttgaa ttgatttccg tcgcgcgacc
      421 gggggtcgct cgtcaacgga atgccgaagt cgagactcaa gggtgaaccc gcgacaaaga
      481 ggcggacgcc gaaaccgaag ttatccgtaa aatccgccgg gctgaagtca taggcgccgg
      541 cgttgacgaa acccgcgtcg tagaagatcg cgaaacggat cgggctcacg atatcgaacg
      601 aatactccgc gctgaaaaac gcatacgcct tgccgccgat cacttcgttg aactcgtcgc
      661 gcggagcgac ttcgcggaat tcgtagccac gcaacgtcgt cgggccgccg agataccagc
      721 gtttgtaagg agcaaccgtc gcactgtcgc cgaagttgtt gatcacgcct gcgcggccga
      781 taaggctgag cacctgcgcc tgggtttcga acacgggaaa aaactgtgat ccgcgcaact
      841 cgagactgta gtaattgtcc tggccgccga gcggtccgcc ggcaacggcc gtgttgacct
      901 cgaggcggtt gccggaggtc gtattgatga tcttgtcgcg cgtgtcgcgc agcagctgga
      961 aaccgatttg cgagatcggg ttgtcaccct cgagcgagcg caggatcggc gaagcgttcg
     1021 gcgcgacgtc ctcgatcgaa atgaccgtgt agctatacga cagccggcct tcgaagagcc
     1081 cgaagaggcg cttgcgaagg taaacttcac caccggtgtc gatttccgta tagaacgtgc
     1141 tgttatattc cgaggtcgtg cgaaacagat ttgtgccgag cgcgagttgc tgctcgaaaa
     1201 gccacggttc ctcaaacgac atcgagactt cgctggaaag cgagccgagc gaaagcttca
     1261 ggcggaactt ctggccgtcg ccttggaaga aggagcggcg gttgaagagg tcgaaattac
     1321 cctgtgagat ttcggcgaac accgtcgcac gctcgag
//
LOCUS       DASPLT010000690         1049 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_32024, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000690 DASPLT010000000
VERSION     DASPLT010000690.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1049)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1049)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1049
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_32024"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..421)
                     /locus_tag="VEA63_07245"
     CDS             complement(<1..421)
                     /locus_tag="VEA63_07245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD83830.1"
                     /translation="MRSLLKRKTDAATSGTPPWHPNFRNFERLPDTKVVRTAFFVNGL
                     AIVVATILLLWFAYQEYQLRDLKRQATEWQEQIDRDRPASNKAIADFKKFQAEGAKVK
                     EIEAFLSARPLISELLVQLGQTLPSNIAIDGFELRGNG"
     gene            complement(393..>1049)
                     /locus_tag="VEA63_07250"
     CDS             complement(393..>1049)
                     /locus_tag="VEA63_07250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD83831.1"
                     /translation="FCGLPSEDIIAIQDSLLAGGIYPERLELASVAMLGGIVDYLAFK
                     KSKTPTLVLEIGAETTHSFIVTAAGVEASRPIAQGLESMVPVVQKELGLKDAESARKL
                     FFSNTFDFTGMGPLLVKKLLKELQSSIGFYEVQTGQSVGQVLCTSIPAKLSWLEGTLA
                     TALGVARLQLELLPWLQSRHVTLAEGVGGAGLDARWLGLFSLMVSQTISDAVPAEKKD
                     "
BASE COUNT          207 a          377 c          275 g          190 t
ORIGIN      
        1 ggccgttgcc gcggagttcg aaaccatcga tcgcgatgtt gctcggcagc gtctgaccga
       61 gttgcaccag caattcggag atcaacggcc gcgccgacag aaaggcctcg atctccttca
      121 ccttcgcgcc ttcggcctgg aactttttga agtccgcgat cgctttgttg ctcgccggcc
      181 ggtcgcggtc gatctgctcc tgccactccg tcgcctgccg cttgaggtcg cgcaactgat
      241 actcctgata cgcgaaccac agcagcagaa tcgtcgccac gacgatcgcc agcccgttca
      301 cgaaaaacgc cgtgcgcacc accttcgtgt ccggcagccg ctcgaaattg cggaagttcg
      361 gatgccacgg cggcgtgccc gaggtcgccg cgtcagtctt tcttttcagc agggaccgca
      421 tcggaaatcg tttgagaaac catcaagctg aacaacccca gccatcgcgc gtccaagccc
      481 gcgccgccca cgccctccgc gagcgtcacg tgacgcgatt gcagccacgg cagcagctcg
      541 agttgcagcc gcgccacgcc gagcgccgtc gcgagcgtgc cttcgagcca gctcaacttc
      601 gccggaatcg aggtgcacaa cacctgcccg accgactgcc ccgtttgcac ttcgtaaaag
      661 ccgatcgagg attgcagctc cttcaaaagc ttcttcacca gcaacgggcc catgccggtg
      721 aaatcgaacg tgtttgaaaa aaacagtttt cgcgccgact ccgcgtcctt cagccccagt
      781 tctttttgca ccaccggcac catcgactcc agcccctgcg cgatcggccg cgacgcctcc
      841 acgcccgccg ccgtgacgat gaacgaatgc gtcgtctccg cgccgatctc caacaccagc
      901 gtcggcgtct tcgacttctt gaacgccagg taatcgacaa tccccccgag catcgcgacc
      961 gacgccagtt cgagccgttc cggataaatg ccgcccgcga gcaacgaatc ctgaatcgcg
     1021 attatgtctt ccgaaggcag cccgcaaaa
//
LOCUS       DASPLT010000691         1238 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_193402, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000691 DASPLT010000000
VERSION     DASPLT010000691.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1238)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1238)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1238
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_193402"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..918)
                     /locus_tag="VEA63_07255"
     CDS             complement(<1..918)
                     /locus_tag="VEA63_07255"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HYD83832.1"
                     /translation="MPSTPRLLQVLALGAALLLLVLLALHLRQPPPAASTHFPSSEPP
                     QTILALNPTFAPSAFASVRSAAELHASLENTLLVRDARPHEAVLTFANAGAYARFLAR
                     AQHRGLAVLGQLDRLLTVRIRYESLSALEAELLEHAADYATIGANHLVHIPAPPAKVD
                     RATAHEIPFRNDTLSFLGVAADHTTWGRGTTIAILDSGVAPDSTFGSGRVQYLNIGLG
                     TVPGTNANDGHGTSVAALAAGASADAAGIAPAATVLSIRVTDTNGTSDIFTVSQAILA
                     AVDAGAPIINLSLGGYTTNTALNSAIAYAH"
     gene            1032..>1238
                     /locus_tag="VEA63_07260"
     CDS             1032..>1238
                     /locus_tag="VEA63_07260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007364114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HYD83833.1"
                     /translation="MKETRLLPAHGNYRELRSFQKAEIVYDLTYRFCERFLRKGDRTV
                     DQMVQAARSGKQNILEGSKASGTSK"
BASE COUNT          241 a          284 c          506 g          207 t
ORIGIN      
        1 gtgggcgtag gcgatggcgg aattcagcgc ggtgttcgtc gtgtagccgc cgaggctgag
       61 attaatgatg ggcgcgccgg cgtcgacggc ggcgaggatc gcttgtgaaa cggtgaagat
      121 gtcgctcgtg ccgttcgtat cggtcacgcg aatactgagg acggtagcgg ctggggcgat
      181 ccccgcggcg tcagccgaag ctccggcggc cagcgcggcg acggatgtgc cgtggccatc
      241 gtttgcgttg gtgccgggca cggtgccgag tccgatattg agatactgca cgcgaccgct
      301 gccgaacgtg gaatcgggcg cgacgcccga gtcgaggatg gcgatggtgg tgccgcgtcc
      361 ccacgtggtg tgatcggccg cgacaccgag gaacgagagc gtgtcgttgc gaaagggaat
      421 ttcgtgagcg gtggcgcgat cgactttcgc cggcggtgca gggatgtgga ccagatggtt
      481 cgcgccgatg gtggcgtagt cggccgcatg ctcgagcagc tcggcttcga gcgcggacaa
      541 ggactcgtag cggatgcgaa ctgtgagcag gcggtcgagt tggccgagaa cagcgagtcc
      601 gcgatgctgt gcacgtgcga gaaaacgtgc gtaagccccc gcgttggcga aggtgagcac
      661 ggcttcgtga ggacgggcgt cgcggacgag gagcgtgttt tcgagggagg cgtggagttc
      721 ggcggcggag cgaacagagg cgaacgctga aggggcaaag gtggggttga gcgcgaggat
      781 cgtttggggc ggctcggagg acggaaagtg ggtggaagcg gctggaggag gctggcggag
      841 gtggagtgcg agaaggacga gcaacagcag cgcggcgccg agagcgagga cttggaggag
      901 ccgcggggtg gagggcatgg gggagaggcg aggagatagg acctatagga caaatggacg
      961 tataggacct atgacggaga cgaatgcaaa atgatgtcgc gagagtggac gggtcttggt
     1021 ggcttttcgc gatgaaggag acgcggttgc tgccggcgca cgggaattat cgggagctgc
     1081 ggtcgtttca gaaggcggag atcgtttatg atctgactta tcgcttttgc gaacggttcc
     1141 tgcggaaagg agatcggacg gtcgatcaga tggtgcaggc tgcccgctcc ggaaaacaaa
     1201 acatcctcga ggggagcaag gcctcgggca cttcgaag
//
LOCUS       DASPLT010000692         1319 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_172658, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000692 DASPLT010000000
VERSION     DASPLT010000692.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1319)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1319)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1319
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_172658"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1240)
                     /locus_tag="VEA63_07265"
     CDS             complement(<1..1240)
                     /locus_tag="VEA63_07265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012372925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD83834.1"
                     /translation="MSASTVSARRPLLAPALVIAGLAIAVLAWTVPVNLKSVSPALLR
                     EAGIDTPSLAAFGEQWVESEKIGPAALVLDAAKALNDPGAATLESSLQELSAQQPEMV
                     AWGGWDPFLDPLFNLRENRGNTASTPVLNFFLPEKARATLLGYLSNSRSLGVQALLKT
                     RAIQATGRFVPVGQAGGQPLDAVILLTALLYQAEHVSPALQREVRALAERAVAQQNLG
                     ELEPFFLDVLSLGKRLDWAQLGELLRRTEDADTVGEYAHLARVTPDELPLIYVAALFS
                     GSADRVASYLIQYGKQGLADLRIALANGQGAVQQLMLRQVPVNHSVTAGLGTAASLVM
                     LHPKAMLVLKYLGYLLGAFLVFRGLDLWIVAPTAGASLGLPRMKSGVLAALFAGLIIV
                     ATEPFLIKAAPPSEFRVRLVL"
BASE COUNT          293 a          460 c          365 g          201 t
ORIGIN      
        1 gtaacacgag ccgcacgcga aattcagaag gcggcgccgc cttgataagg aacggttcgg
       61 tggcgacgat gatcagcccg gcaaacaatg cggcaagcac accgcttttc attcgcggca
      121 aaccgagact cgcgcccgcc gtcggtgcca cgatccagag atcgagcccg cggaaaacca
      181 gaaaagcgcc gagcagatag ccgagatatt tcagcacgag catcgctttc ggatgcagca
      241 tcacgagcga cgccgccgtg cccaatcccg ccgtgaccga atgattcacc ggcacctgcc
      301 gcagcatcaa ctgctgaacc gcgccctgac cgttcgccag cgcgatgcgc aaatccgcaa
      361 gaccctgttt tccatattga ataagataac tcgccacgcg atccgccgaa cccgagaaca
      421 gcgccgccac ataaatcaac ggcagctcgt cgggcgtcac ccgcgcgagg tgcgcgtatt
      481 cccctacggt gtccgcgtcc tccgttcgtc gaagcagttc gccgagttgc gcccaatcga
      541 gacgtttccc cagcgacagc acatcgagga aaaacggctc cagttcaccg agattctgct
      601 gcgcaacggc gcgctcggcg agcgcacgca cctctcgctg caacgccggc gacacgtgct
      661 cggcttgata aagcaatgcc gtcagcaaaa tcaccgcatc gagcggctgt ccgcccgcct
      721 gcccgaccgg aacgaagcgc ccggtcgcct gaatggcgcg cgtcttcagc agcgcctgca
      781 cgccgagcga gcgcgaattc gaaagataac cgagaagcgt cgcgcgcgct ttctccggca
      841 ggaaaaaatt taaaaccggc gtgctcgccg tgttcccgcg attttcgcgg agattgaaca
      901 agggatcgag aaacggatcc cagccgcccc acgcaaccat ctccggctgc tgcgcggaaa
      961 gttcctgcaa cgaagactcg agcgtggccg cgcccggatc gttcaacgcc ttggccgcat
     1021 cgagcaccaa cgccgccggg ccgatcttct ccgactccac ccactgctcg ccaaacgccg
     1081 ccagcgaagg cgtgtcgatt cccgcctcgc gcaacagcgc cgggctcacc gatttcaggt
     1141 tcaccggcac cgtccacgcg agcacggcga tcgccaggcc cgcgatcaca agcgccggcg
     1201 caagcaacgg ccttctggcg gaaacggttg aggcgctcat tgaaaggtag tattggtttg
     1261 cagagctaaa tgacaaaccc acgctcctgc ccctaaccga ccgatgccac tgccaattg
//
LOCUS       DASPLT010000693         1061 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_32083, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000693 DASPLT010000000
VERSION     DASPLT010000693.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1061)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1061)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1061
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_32083"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..334)
                     /locus_tag="VEA63_07270"
     CDS             complement(<1..334)
                     /locus_tag="VEA63_07270"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007359231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HYD83835.1"
                     /translation="MKKALITGITGQDGSYLAELLLSKGYEVHGVIRRASSFNTGRID
                     HLYEDRNVYGKRLFLHYGDLADSVQMVKLLYELQPDEIYNLGAQSHVRVSFDVPEYTG
                     DVDGLGAQR"
     gene            606..>1061
                     /locus_tag="VEA63_07275"
     CDS             606..>1061
                     /locus_tag="VEA63_07275"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HYD83836.1"
                     /translation="MPFISVNPATGRRIGTHRAHSQRTIEEIVSRAHAAFGTWRTTTL
                     AARARTLRAIAKALRREQEPLAALITAEVGKPIAQSRAEVAKSALACDYYAEHGRRWL
                     APAHPPGAPKNTAVHFEPLGPVLAVMPWNFPIWQAMRAIAPALVAGDTFL"
BASE COUNT          205 a          364 c          298 g          194 t
ORIGIN      
        1 tgcgctgcgc gccgagtcca tcgacgtcgc ccgtgtattc cggcacgtcg aacgaaacgc
       61 gcacgtgcga ctgcgcgccg agattgtaga tctcatccgg ctgcaactcg taaagcaact
      121 tcaccatttg cacggaatcg gcgagatcgc cgtagtgcaa aaagagccgc ttcccgtaga
      181 cgtttcgatc ctcgtaaagg tgatcgatgc gaccggtgtt gaagctcgat gcacggcgaa
      241 tgacgccatg cacctcatag cctttggaga gaagcagctc cgccagatag gaaccatcct
      301 ggccggtgat gccggtaatc agcgcttttt tcattcgtta aaggggacag taggatttga
      361 ttccgacccg gcaagttccg tcccggctta actttgggcg aaatacaaaa tcagggagac
      421 cctgaggcgt gttcctggaa acaaattttc acccctcgga caaggtggag cccgacgtcc
      481 cgtcgggctg attgaatcgc gaacgtttcc aatccagccg acgagaacgc cggaccccaa
      541 cccgcaattc cccgctcgtg ctcccccgag ttgctgcgtg cgccttttca cactgttgtg
      601 ccgccatgcc gttcatttcc gtcaaccccg ccaccggccg gcgcatcggc acgcatcgcg
      661 cgcattcgca gcgcaccatt gaggaaatcg tttcgcgcgc gcatgccgcc ttcggcacgt
      721 ggcgcaccac caccctcgcc gcgcgcgccc gcacgctgcg ggccatcgcg aaagcgttgc
      781 gccgcgagca agagccgctc gccgcattga tcaccgcgga agtcggcaag cccatcgcgc
      841 aatctcgcgc cgaagtggcg aagagcgccc tcgcctgcga ctactacgcg gaacacggca
      901 gacgctggct cgcgcctgcg catccgcccg gcgcaccgaa aaacaccgcg gtccatttcg
      961 agccactcgg ccccgtgctc gccgtgatgc cgtggaattt tccgatctgg caggcgatgc
     1021 gcgcgatcgc gcctgcactc gtcgccggtg acaccttcct c
//
LOCUS       DASPLT010000694         3426 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_151600, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000694 DASPLT010000000
VERSION     DASPLT010000694.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 3426)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3426)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3426
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_151600"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1048)
                     /locus_tag="VEA63_07280"
     CDS             complement(<1..1048)
                     /locus_tag="VEA63_07280"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HYD83837.1"
                     /translation="MNHHARSFLIVFLFALAIPLLAQTPVGTLTGRVTDDSTRLMLGG
                     ARVGVIGTPLETYADVSGRYMLPNVPSGPQTLVVSYVGYGELRQAVTVAAGSVTEADF
                     SFGRDVVRLDRFVIEGSLVGAARALNQQRSADTLVNVVASDEIGNFPDQNAAESLQRI
                     PGVSLYRDQGEGRFVLVRGINYTMGNVTLNGAKLASPETGERGIALDVLPADALASIE
                     VTKVATPDMDGEGLGGQVNLRTKSAFETSGLAASFTAQGHYSALADAYSSKFNASVSN
                     VFADGTWGVIVSPTWQRRKFGSNNFEIDDGWTDEVEDGDDNERELPAYFLQDISFREY
                     EIERERYGVSGALEF"
     gene            complement(1194..3041)
                     /locus_tag="VEA63_07285"
     CDS             complement(1194..3041)
                     /locus_tag="VEA63_07285"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HYD83838.1"
                     /translation="MMRNLPIRQKLAIVILTTSSAALLITCLSFFAYEFVTFRRTAVR
                     NLATLGEIIASNSTAALAFDNPDDARETLSALRAERHIVAAALYDASGAIFAKYPAGL
                     ADSAVPAAPGKTGHRITLRSLTSFQPVVHNDRQLGILYLESDMDAMVERFQLYALIAM
                     IVMGGSLLGAFVLSQKLQHQISRPVLALADTARAVSDRRDFSVRAVRQGDDELGVLTD
                     AFNDMLTQIHEQDRALRESEARLRAVLNSAVSAVVVTDHLGRIIDWNACAERMFGRTR
                     GEALGLDLGEVLEPVADGAVAGMKRFMTKADSEITTWPIEMNGHRRDGEIFPIEVAIN
                     PVVSGGVVTFCAFASDITERKKSEIEIQTLNQQLERRVAERTEQLQSANKELEAFSYS
                     VSHDLRAPLRHIDGFANMLTTHVGDALDEKSRRYLTVIMDSAKRMGRLIDDLLSFSRH
                     GRAELRHVPVKLGELVSEVRRQMLDDVGGRNITWKIEELPEVEGDIAMLHQVFANLLG
                     NAVKYTRQRSEAVIEVGSRTNEEGELVVFVRDNGAGFNPKYVDRLFGVFQRLHSESEF
                     EGTGVGLANVQRIIHRHGGRVWAEGQVNEGATFYFTLPRARRLDGGAVV"
     gene            complement(3046..>3426)
                     /locus_tag="VEA63_07290"
     CDS             complement(3046..>3426)
                     /locus_tag="VEA63_07290"
                     /inference="COORDINATES: protein motif:HMM:NF025069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiR family protein"
                     /protein_id="HYD83839.1"
                     /translation="TPIIIGVLGQDPFGQFLDQLVSGEVVNGRPLVVQRFRSVAEIQA
                     CHVLFVSGSEGKRGQQIIASLRGRSILTVCDWEGLMDQGAMVQFVTERNRVRLRINLD
                     SVKAAGLTISSKLLRSAETITHAR"
BASE COUNT          655 a         1209 c          938 g          624 t
ORIGIN      
        1 tgaactccag cgcgccgctg acgccatagc gctcgcgctc gatctcgtat tcgcgaaacg
       61 aaatatcctg caggaaatac gcgggcagtt cccgctcgtt gtcgtcgccg tcctccactt
      121 catcggtcca gccgtcgtcg atttcgaaat tgttcgaacc gaacttccgc cgctgccacg
      181 tcggcgagac gatcacgccc cacgtgccgt cggcgaacac gttcgaaacc gacgcgttga
      241 acttgctgct gtaagcatcg gcgagcgccg agtagtgacc ttgcgcggtg aacgaggcgg
      301 cgagaccgct cgtctcgaac gcgcttttcg tgcgcagatt cacctgcccg ccgagcccct
      361 cgccgtccat gtccggtgtc gccactttcg tgacttcgat cgacgccagg gcgtccgccg
      421 gcaacacatc gagtgcgatg ccacgttcgc ccgtctccgg actcgcgagc ttcgcgccgt
      481 tcaacgtcac gttgcccatc gtgtaattga tgccacgcac gagcacgaaa cggccttcgc
      541 cttggtcgcg ataaagcgac acgcccggaa tccgctgcaa cgactccgcc gcgttctgat
      601 ccggaaaatt tccaatctcg tccgatgcaa cgacgttcac gagcgtgtct gcgctgcgct
      661 gttgattgag cgcgcgagcc gcgccgacca gcgagccttc gatcacgaaa cgatcgagtc
      721 gcacgacgtc gcgaccgaac gaaaaatccg cttccgtcac cgaacccgcc gcaaccgtca
      781 cggcctgacg caactcgccg tagcccacat aactcacgac cagcgtttgc ggaccgctcg
      841 gcacattcgg gagcatgtag cggcccgaca cgtcggcata cgtttcaagt ggcgtgccga
      901 tcacccccac cctggctcct ccgagcatca gtcgcgtcga gtcgtctgtg actcgacccg
      961 tgagcgttcc gaccggcgtt tgagccagca acggaatcgc caacgcaaat aggaacacga
     1021 tcaagaagct gcgtgcgtgg tggttcatca aaaccgaccg tgcgacgcgc ggcaaaccgc
     1081 ggtcaacagc gcgcgttcgg cgtcaccgaa ctactaccgt cccgtcaccc gtccgtcgca
     1141 aaacgggatc cgactcgccg ttgggatctt cctcttcgcc tcaagccgcg acgtcacacc
     1201 accgcgccgc cgtcgagtcg ccgagcccgc ggcagcgtga aatagaacgt cgcaccttcg
     1261 ttcacctgac cctccgccca gacgcgacca ccgtgccgat ggatgatgcg ctgcacattc
     1321 gcgagaccga cgcccgtgcc ttcgaactcg ctctccgaat gcagccgctg aaacacgccg
     1381 aacagccggt ccacatactt cggattgaaa cccgcgccat tgtcgcgaac gaaaaccacc
     1441 aactcacctt cctcgttggt gcgagagccg acttcgatta cggcctcgct ccgttgacgc
     1501 gtgtatttga ctgcgttgcc gagcagattc gcgaacactt gatggagcat cgcgatgtcg
     1561 ccttcaacct ccggtaattc ctcgatcttc caggtgatgt tgcggcctcc gacgtcgtcg
     1621 agcatttgcc gccgcacctc gctcaccaac tcgccgagct tcacgggcac atgccgcaac
     1681 tccgcgcggc cgtgtcgcga aaaactcaac agatcgtcga tcaatcggcc catgcgcttc
     1741 gccgagtcca tgatcaccgt gaggtagcgg cggctcttct cgtcgagcgc gtcgccgacg
     1801 tgcgtcgtca gcatgttggc gaagccgtcg atgtgccgca gcggtgcgcg caggtcatgg
     1861 gaaactgaat acgagaacgc ctccaactcc ttgttcgcgc tttgcaactg ctcggtgcgc
     1921 tcggcgacgc gccgctccaa ctgctggttg agagtttgga tttcgatctc ggactttttc
     1981 cgctccgtga tgtcgctcgc gaacgcgcaa aacgtcacca cgccgcccga caccaccgga
     2041 ttgatcgcca cctcgatcgg gaatatttct ccgtcacgcc ggtgaccgtt catctcgatc
     2101 ggccaggtgg tgatttcgct gtccgctttc gtcatgaacc gcttcatccc ggcgaccgcc
     2161 ccgtcagcca ccggctccaa aacctcgccg agatcgagcc ccagcgcttc accgcgcgtg
     2221 cggccaaaca tgcgctcggc gcaggcattc caatcgatga tccggcccag gtggtcagtc
     2281 accaccaccg cgctgaccgc ggaattcaac accgcgcgca accgcgcctc actctcgcgc
     2341 aacgctcgat cctgctcgtg gatttgcgtc agcatgtcgt tgaacgcgtc ggtcaacacc
     2401 ccgagttcgt cgtccccctg gcgcacggcg cgcacggaga aatcgcggcg gtccgacacc
     2461 gcccgcgccg tgtccgcgag cgcgagcacc ggccgggaaa tctgatgctg cagtttttgc
     2521 gaaagcacaa acgcgcccaa cagtgatccc cccatcacga tcatcgcgat cagggcgtag
     2581 agctgaaacc gctcgaccat cgcgtccatg tccgactcca gataaaggat gccgagttgc
     2641 cggtcgttgt gcacgaccgg ctggaacgag gtcagcgagc gaagcgtgat ccgatggccg
     2701 gtttttcccg gtgccgctgg caccgcgctg tcggccagcc cggcgggata cttcgcgaaa
     2761 atcgcacccg aggcatcgta aagcgccgcc gccacgatgt gccgctccgc gcgcagggcc
     2821 gagagcgttt cacgcgcgtc gtcgggattg tcgaacgcga gcgccgccgt gctgttcgaa
     2881 gcgatgatct cgcccaacgt cgccaggttc ctcaccgccg tgcgacgaaa cgtgacgaac
     2941 tcgtaggcga aaaacgacag gcacgtgatc aacaacgccg cgctgctcgt cgtcaggatg
     3001 acgatcgcca gcttctgccg aatgggaaga ttgcgcatca tgccgtcacc tcgcgtgtgt
     3061 gattgtttca gccgaacgca gcagcttcga actgatcgtc agccccgccg cttttacgct
     3121 gtccagattg atgcgcagcc gcacgcgatt gcgttccgtt acgaactgca ccatcgcccc
     3181 ctgatccatc aggccctccc agtcgcacac cgtcagaata ctgcggccgc gcaggctcgc
     3241 gatgatctgc tggccccgtt ttccctccga gccgctcacg aacagcacgt gacacgcctg
     3301 gatctcggcg accgaacgga aacgctgcac caccaacggg cgcccattca ccacctcccc
     3361 gctcaccaac tgatccagaa actggccgaa cggatcctgc cccaaaacac cgatgataat
     3421 cggcgt
//
LOCUS       DASPLT010000695         1085 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_274172, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000695 DASPLT010000000
VERSION     DASPLT010000695.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1085)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1085)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1085
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_274172"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            87..>1085
                     /locus_tag="VEA63_07295"
     CDS             87..>1085
                     /locus_tag="VEA63_07295"
                     /inference="COORDINATES: protein motif:HMM:NF013752.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HYD83840.1"
                     /translation="MTWIVNDGAIFDHLPRHGNISWAPSQLILSAILWVWSDQAQLTV
                     AYHEAHQAVRGFFPRVALQSYQGFIRALVRWTEPLRPLLWARLHALMEQVAGGYWRCG
                     EYLALAVDGSRVTTPRTKSNERAFAAQNFGRGRKARSRRKWKNKKRRSKKLCIPVKPQ
                     IWLTMIWHMGLKMPWCWKTGPSTASERGHFLDLLKNCAFPRKTLFCADAGFVGYELWK
                     AILDGGHDFLIRVGGNVTLLRKLGRVRVDRDLVYFWPKTAQEKKRPPLVLRLLSFSGP
                     RGPVHLLTSVLDARRLSLANARELYRQRWGVELQFRTLKQTFGRSKLRSRTADRALV"
BASE COUNT          204 a          327 c          337 g          217 t
ORIGIN      
        1 cgcgaaagcg acgcgcgaaa aatcgcggtt cttcagccac atcggcctcc gcccaagttt
       61 gcaacctgga gttgttgcga cgggcgttga cgtggattgt gaacgacggg gccatcttcg
      121 atcatctgcc gcggcacggc aatatcagtt gggcgccgtc gcaacttatc ctctcggcca
      181 ttctctgggt gtggtcggat caggcgcagt tgacggtcgc atatcacgaa gctcatcagg
      241 cggtacgggg attctttccg cgggtcgcgc tgcagagcta tcaaggcttc attcgagccc
      301 tggtccgttg gaccgagccg ttgcggccgt tgctgtgggc gcggctgcac gcgctcatgg
      361 aacaagtagc cggcgggtat tggcgttgcg gcgagtatct tgcgctcgcg gtggacggtt
      421 cgcgggtgac gacgccgcgc accaagagta acgaacgcgc ctttgccgcg caaaacttcg
      481 gccgcggccg aaaagcccgt tcgcgacgaa aatggaagaa taaaaaacgc cgcagcaaga
      541 aactgtgcat tccggtgaag ccgcagatct ggctcacgat gatctggcac atggggctga
      601 agatgccttg gtgttggaag acggggccgt cgaccgcgag cgagcgaggc cactttctcg
      661 acctgctgaa aaactgtgct tttccgagga aaacgctgtt ttgcgcggat gccggcttcg
      721 tcggctacga actttggaaa gcgattctcg acggcggaca cgactttttg attcgcgtcg
      781 gcggcaacgt aacgctgctc cgcaaactcg gacgagtgcg cgtcgatcgc gacttggtct
      841 acttctggcc gaagaccgca caagagaaaa agcggccccc gctcgtgctg cgattattgt
      901 ccttctccgg ccctcgcggc cccgtgcatc tcctcacctc cgtcctcgac gcgcgccgcc
      961 tgtctttggc gaacgctcgc gaactgtacc ggcaacgttg gggcgtcgaa cttcagttcc
     1021 gcacgctcaa gcaaaccttc ggccggagca aacttcgcag ccgtaccgcg gatcgcgcgc
     1081 tcgtg
//
LOCUS       DASPLT010000696         2012 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_32162, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000696 DASPLT010000000
VERSION     DASPLT010000696.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 2012)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2012)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2012
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_32162"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..601
                     /locus_tag="VEA63_07300"
     CDS             <1..601
                     /locus_tag="VEA63_07300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007364245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HYD83841.1"
                     /translation="AAVRGVDWRAVVDGLRPHSQAIWQGFSAEERSRFLRYARSFWEG
                     HRHRIASQLAEVLVRMEAEGRLKYHAGRLQTLDVDASGVDAVFRRRGTIQHVALRVVK
                     VVNCTGPRTDYSKYQHPLFIHLLARGLIDHDPLALGISARPTGEVLRYRGDEVGWLFT
                     LGAPLKGVLWETTSVREIREQASALATRLLASEVDAGAR"
     gene            complement(777..1703)
                     /locus_tag="VEA63_07305"
     CDS             complement(777..1703)
                     /locus_tag="VEA63_07305"
                     /EC_number="1.11.1.5"
                     /inference="COORDINATES: protein motif:HMM:NF015131.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HYD83842.1"
                     /translation="MHRLRFVLAWTFVAAAHASDPTDAAALGGKLFFDPILSADRTVS
                     CASCHKPEYAFADNAKVSLGLHGQPGTRNTPSAMNVSGRLAFFWDERAGSLEEQALAP
                     IANPAEMGLPVDEAVARLNADEIYRRAFATIFEGPATEKNLARALAEFERTLETADTP
                     YDRYIAGDDSALSPEAARGRLLFIGKANCANCHSGEDFTADRPKSIGLYNAKELNDPG
                     RGGVTQNPEHMGLFKTPSLRNVAVTAPYMHNGMFTTLREVVDYYNHPDRVVAHSINRD
                     KALDTPLNLTDTEIHELVAFLESLTDDRFVKR"
     gene            complement(1703..>2012)
                     /locus_tag="VEA63_07310"
     CDS             complement(1703..>2012)
                     /locus_tag="VEA63_07310"
                     /inference="COORDINATES: protein motif:HMM:NF022853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD
                     domain-containing protein"
                     /protein_id="HYD83843.1"
                     /translation="LYSSDFKADIGPLPTAFTFEPSSGAAPVGEVRAINPKRKKDRTW
                     DTEFTYFENTAEFRQPLRVAKSGFTLRGTIKGQLCNERDGTCSLFEQPLEITASTDRS
                     "
BASE COUNT          322 a          520 c          768 g          402 t
ORIGIN      
        1 ggctgcggtt cgcggtgtgg attggcgcgc ggtcgtcgac ggcttgcgcc cgcattcgca
       61 ggcgatctgg cagggatttt ccgcggaaga acgcagccgg tttttgcggt atgcgcgttc
      121 gttttgggaa gggcatcggc atcggatcgc gtcgcagctc gcggaggtgc tggtgcggat
      181 ggaagcggaa ggccgtttga aatatcacgc gggccgtttg cagacgctgg atgtcgacgc
      241 gagcggcgtg gatgcggtgt ttcgccggcg aggaacgatt cagcacgtgg cgctgcgggt
      301 ggtgaaggtc gtcaattgca cgggaccgcg gacggattac tcgaagtatc agcatccgtt
      361 gttcattcat ctgctcgcgc gcgggctgat cgatcacgat ccgctggcgc tgggtatcag
      421 cgcccggcca accggagagg tgctgcgcta tcgcggcgac gaagtcggct ggctgttcac
      481 actcggcgcg ccgttgaaag gcgtgttgtg ggaaacgact tccgtgcgcg aaatccgcga
      541 gcaagcctcg gcgctggcga cacgtttgct ggcgagcgag gtggatgcgg gcgcgaggtg
      601 aacgctgtcc gggagaatgt taaaacaaga aggtagggac ggctgtcccc aggcgtcctg
      661 cgtttgacgc cggctttcag tgggtgcggg tagccaagtc ccggcccgct cggagaacgg
      721 gccctgccgg cgctgccgcg ccatcgaatt tttcgtggct ctacggccgg cagtttttat
      781 cgtttcacga agcggtcgtc ggtgagggat tcgaggaagg cgacgagttc gtggatttcg
      841 gtgtcggtga gattgagcgg cgtgtcgaga gccttgtcgc gattgatgct gtgcgccaca
      901 acacggtcgg gatggttgta gtagtcgacc acctcgcgga gggtggtgaa catgccgttg
      961 tgcatgtagg gcgcggtgac ggcgacgttg cgcagcgagg gcgttttgaa gaggcccatg
     1021 tgctcgggat tttgggtaac accgccgcgg ccggggtcgt tcagttcctt ggcgttgtag
     1081 aggccgatgc ttttcggtcg gtcggcggtg aagtcctcgc cggagtggca gttcgcgcaa
     1141 ttggccttgc cgatgaaaag cagacgcccg cgcgcggctt ccggcgagag cgcggagtcg
     1201 tcgccggcga tgtagcgatc gtagggcgtg tcggcggttt cgagcgtgcg ttcgaactcg
     1261 gcgagggcgc gggcgagatt tttttcggtg gcgggacctt cgaaaatcgt ggcgaaggcg
     1321 cggcgataaa tttcgtcggc gttgaggcgg gcgacggctt cgtcgacggg caggcccatc
     1381 tcggcgggat tcgcgatggg agcgagggct tgttcctcga gcgagccggc gcgttcgtcc
     1441 cagaagaacg cgagccggcc ggagacgttc atcgcggagg gcgtgttacg cgtgccgggt
     1501 tggccgtgaa ggccaaggct gaccttggcg ttgtcggcga aggcgtattc aggtttgtga
     1561 cacgacgcgc agctcacggt gcggtcggcg gagaggatcg gatcgaagaa cagtttcccg
     1621 ccgagcgcgg cggcgtcggt ggggtccgac gcgtgcgccg ctgcgacgaa tgtccacgcg
     1681 aggacgaaac ggaggcgatg catcacgaac gatcggtcga ggcggtgatt tccaagggtt
     1741 gctcgaacaa cgagcaagtg ccgtcgcgct cgttgcagag ctggcctttg atcgtgccgc
     1801 gaagggtgaa gccggatttc gcgacgcgca gcggctgacg gaattcggcg gtgttttcga
     1861 aatacgtgaa ctccgtgtcc cacgtccgat cttttttccg cttcggattg atcgctcgga
     1921 cttcgccgac gggcgcggcg ccggaggagg gctcgaacgt gaaggcggtg gggagcggac
     1981 cgatgtcggc tttgaagtcg ctcgagtaga gc
//
LOCUS       DASPLT010000697         1337 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_9003, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000697 DASPLT010000000
VERSION     DASPLT010000697.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1337)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1337)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1337
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9003"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..870)
                     /locus_tag="VEA63_07315"
     CDS             complement(<1..870)
                     /locus_tag="VEA63_07315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="heparinase"
                     /protein_id="HYD83844.1"
                     /translation="MRYLFHPLAVLLWATVAIAAEDPLGTLRRDHPRLLFNQAEFEAK
                     RAAAENDPLRAELHRQIVAAAEQQLSEPPIRYVLVGPRMLDQSRRALAHVTTSAMAYR
                     LSGDARFAAHAKQVMLTAAAFPDWNPSHFLDVAEMATALGLGYDWLYDELSADERAAI
                     KRALLDKALAYVKPAYARVDPTRISFPFVQGNLTNNWNQVCNGGFLVAALAIAEDEPE
                     LARAVIAGVRETLPHAMEAYRPDGAYPEGPVYWGYGTRYNVLILAALESALGNDFGLG
                     EFPAFDRTALFRFY"
     gene            complement(1035..>1337)
                     /locus_tag="VEA63_07320"
     CDS             complement(1035..>1337)
                     /locus_tag="VEA63_07320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD83845.1"
                     /translation="PFIRDIYRYYGAEDNVANVHLANEGHDYGPSKREAMYRFVAPRL
                     GLDLSEVLDANGAIDETKVTIEPHEKMHFFDDTHPVPERAMRTAAAVEAVLKELQR"
BASE COUNT          264 a          459 c          380 g          234 t
ORIGIN      
        1 ataaaaccgg aacagcgccg tgcggtcgaa cgccggaaat tcacccaaac caaaatcgtt
       61 gcccaacgca ctctcgagcg ccgctaaaat cagcacgttg tagcgcgtgc catatcccca
      121 atacaccggc ccctcgggat acgcgccgtc cggccgataa gcttccatcg cgtgcggcaa
      181 cgtctcgcgc acgcctgcga tgacagcgcg cgccagttcc ggctcgtcct ccgcgatcgc
      241 gagtgctgcg accaaaaagc cgccgttgca cacttgattc cagttgttcg tcagattgcc
      301 ctgcacgaac ggaaacgaaa tccgcgtcgg gtccacgcgc gcataggcgg gcttcacgta
      361 agcgagcgcc ttgtcgagga gcgcgcgttt gatcgcggcg cgctcgtcgg ccgacagctc
      421 gtcgtagagc cagtcgtagc ccaatcccag cgccgtcgcc atctccgcca cgtcgagaaa
      481 gtgcgagggg ttccaatcgg gaaacgccgc cgccgtgagc atcacctgct tcgcgtgcgc
      541 cgcgaaccgc gcgtctccac tcaaccgata cgccatcgcc gaggtggtca cgtgcgccag
      601 cgcgcgacgc gattggtcga gcatccgcgg accgacgagc acatagcgga tcggcggctc
      661 gctcaactgc tgctctgccg cggcgacgat ttgccggtgc aattccgccc gcaacggatc
      721 gttctccgcc gcagcgcgct tcgcctcgaa ctccgcctgg ttgaacagca gcctcgggtg
      781 gtcccgccgc aacgtgccga gcggatcttc ggcggcaatg gcaaccgtcg cccacaacaa
      841 gaccgccaac gggtggaaga ggtaacgcat gaagcaaggt tgggtcggcg tagcgacacc
      901 ggccacgccg aaaattggca accaagctga agcatggatt cacgttggcg aacccaacaa
      961 agctttagcg tatagcagtg cgccccgcca tcgcatcacg gcggcgtttt ttccccgtgc
     1021 tttcagcacc gcaactaacg ctgcaattcc ttcagcaccg cttccaccgc cgccgccgtg
     1081 cgcatcgctc gctcaggcac aggatgcgtg tcgtcgaaaa aatgcatttt ctcgtgcggc
     1141 tcgatggtga ccttcgtctc gtcgatcgcc ccattcgcat cgagcacctc gctcaaatct
     1201 aaaccgagcc gcggcgctac gaagcgatac atggcctcgc gcttcgatgg gccgtagtcg
     1261 tgcccttcgt tcgccagatg aacgttcgcc acgttgtcct cggcgccgta gtaacgatag
     1321 atgtcgcgaa taaacgg
//
LOCUS       DASPLT010000698         1061 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_32187, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000698 DASPLT010000000
VERSION     DASPLT010000698.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1061)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1061)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1061
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_32187"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            389..796
                     /locus_tag="VEA63_07325"
     CDS             389..796
                     /locus_tag="VEA63_07325"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HYD83846.1"
                     /translation="MDSLQLEFHHESGILVGKLSGRLDGNSAPEFGRRVAEELAAGPI
                     PVVLDVTDLAFISSAGLREFVGLAKRLSPAKLKASLVGVSPTVSEVLDISGLTALFLR
                     LDSVESAKQQVAGGGTGSRNGLLGRLFSGRPKS"
     gene            793..>1061
                     /locus_tag="VEA63_07330"
     CDS             793..>1061
                     /locus_tag="VEA63_07330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD83847.1"
                     /translation="MTIPAEYRAGYERARQVDPVLTERYLRHTTVGDPAADALIEALN
                     RQPREHAHRWIEGGIEGKADALRDAPDGVRDFFAALEPVPDWWNA"
BASE COUNT          184 a          332 c          347 g          198 t
ORIGIN      
        1 gaggcatgca cgaattccgt gaacggccga aaacggcgct aggctcccgg catcggtgga
       61 accacggtga gcagaggcat tcgaaacggg atgtccatcg gtcggtaaac gtcaatgatg
      121 cccgctgcgc cgcggagaaa cgcgacgcgt gaaggagcgg agttcaccac gctgcggccg
      181 agttcctctt cgccgtgtcg agagtcaact ccgtccgtcc ggcccgaagc tcgccgcacc
      241 gcgtgacaat ctcaagcccg ccccggcagt gcggctcgtg gagacttacg cgttaccctg
      301 gcttgccatc gcgcctgctg acgacgccgc gtgcttttga cggcaataaa tacttgttcg
      361 gccagcgaat cgcgcaatct tcgcgaggat ggattctctc cagctggaat ttcatcatga
      421 gtccggaatc ttggtgggta agctgagcgg ccgcttggac ggaaattctg cgcctgagtt
      481 cggtcgccgg gtcgcggaag agctggccgc cggcccaatc cccgtcgtgc tggacgttac
      541 cgacctggcc tttatcagca gtgccggact gagggaattc gtgggactgg ccaaacgcct
      601 ttctccggcc aaactcaagg cctcgctggt cggtgtttcc cccaccgtga gcgaggtgtt
      661 ggatatttcc gggctgactg cgcttttcct tcgtctcgac tccgtcgaga gcgcgaagca
      721 acaggtcgct ggcggcggga cgggctcccg caacggtctg ctgggacgcc tgttttccgg
      781 ccgacccaag tcatgacgat ccccgcggag tatcgcgcgg gttatgagcg cgcgcggcag
      841 gtcgatccgg ttttgacgga gcgctacctc aggcacacca ccgtcggcga tcctgcggcg
      901 gacgccctga tcgaagctct caatcgacag ccgcgcgagc acgcgcaccg gtggatcgag
      961 ggcggcatcg agggcaaggc ggacgcgcta cgcgatgcgc cggacggcgt gcgcgacttc
     1021 tttgccgcgc tcgaaccggt ccctgattgg tggaatgcga g
//
LOCUS       DASPLT010000699         1302 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_295389, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000699 DASPLT010000000
VERSION     DASPLT010000699.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1302)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1302)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1302
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_295389"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            133..>1302
                     /locus_tag="VEA63_07335"
     CDS             133..>1302
                     /locus_tag="VEA63_07335"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008740 - L-rhamnose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HYD83848.1"
                     /translation="MSSSKQISAAYKLARETYAEFGVDTETAIKRALSIPISLHCWQT
                     DDVRGLETPKSGVDSGGIMATGGYAGRARNGDEMRADLDLVLKLLPGKQRLNLHASYA
                     ETGSAHIDRDELAPEHFSRWTDWAKSRRIGLDFNPTYFAHPNATSGFTLSHADASIRK
                     FWIAHAKACRRIAQSFAKKLGTPCVHNHWIPDGAKDAPADRWAPRQRLVESFDAIFAD
                     KSIDRKLCVDAVEGKLFGLGSEEYVVGSQEFYSAYAQARDLVLCLDLGHYHPTESVAD
                     KVSALMQFHERILLHTSRPIRWDSDHVVILDDPTRNLFLEIARGNVWDRVFVALDFFD
                     ASINRIAAYIVGTRATRQAILLGLLDPSKQLRAAESAGCGHERLALMEQAKALPWG"
BASE COUNT          256 a          485 c          331 g          230 t
ORIGIN      
        1 caccttccgc gcctcgctcg ccaaacaaat caaacagacc gtgtatcagc cgagagccca
       61 gcgctaaaga gccgagagcc aaccgtcttc ttgcaacctt ttcggctctc cgctcgcagc
      121 taattttcca ccatgtcttc ctccaaacaa atctccgccg cctacaaact cgcgcgcgaa
      181 acttacgccg agttcggcgt cgacaccgaa accgcgatca agcgcgcgct ctccattcca
      241 atctcgctgc attgctggca gaccgacgac gtgcgcgggc tcgaaacacc gaaatccggc
      301 gtcgattccg gcggcatcat ggcgaccggt ggctacgcgg gccgcgcgcg caacggcgac
      361 gagatgcgcg ccgacctcga tctcgtcctg aaacttctcc ctggcaaaca acggctcaac
      421 cttcacgcca gctacgccga aaccggctcc gcgcacattg atcgcgacga gctcgcgccc
      481 gaacatttct cgcgctggac cgactgggcg aagtcccgcc gcatcggcct cgatttcaac
      541 ccgacctatt tcgcgcatcc caacgccacc tccggcttca cgctctcgca cgccgacgcc
      601 tcgatccgca aattctggat cgcacacgcc aaagcctgcc ggcgcatcgc gcaaagtttc
      661 gccaagaaac tcggcacgcc ctgcgtccat aatcactgga tacccgacgg cgccaaagac
      721 gcacccgccg atcgctgggc gccgcgccag cgcctcgtcg agtccttcga tgcgatcttc
      781 gccgacaaat cgatcgaccg aaaactctgc gtcgacgccg tggaaggaaa actcttcggc
      841 ctcggcagcg aggaatacgt cgtcggctcg caggagtttt attccgccta cgcgcaagct
      901 cgcgatctcg tgctctgcct cgacctcggc cattatcacc cgaccgaatc cgtcgcggac
      961 aaagtctccg cgctcatgca gtttcacgaa cgcatccttc tgcacacgtc gcgaccgatc
     1021 cgttgggaca gcgatcacgt cgtgattctc gacgacccga cgcgcaacct cttcctcgaa
     1081 atcgcgcgcg gcaatgtctg ggaccgcgtc ttcgtcgcgc tcgatttctt cgatgcctcg
     1141 atcaaccgca tcgccgccta catcgtcggc acgcgcgcca cgcgccaggc gattctcctc
     1201 ggcctgctcg atccatcgaa acaactgcgc gccgctgaat ccgccggctg cggccatgag
     1261 cggctcgcgt tgatggagca ggccaaagcg ctgccatggg gc
//
LOCUS       DASPLT010000700         1009 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_90969, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000700 DASPLT010000000
VERSION     DASPLT010000700.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1009)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1009)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1009
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_90969"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1009)
                     /locus_tag="VEA63_07340"
     CDS             complement(<1..>1009)
                     /locus_tag="VEA63_07340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007363937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140658 - ATP-dependent chromatin
                     remodeler activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SNF2-related protein"
                     /protein_id="HYD83849.1"
                     /translation="ADEVGLGKTIEACLIVQRLLAVGKARRVLILVPESLTHQWFVEL
                     LRRFNLWFSIYDEARCAALEESDPGENPFEASQLALCSVGFLAADERRREQAVAAGWD
                     MVVVDEAHHLAWSPEGASAEYQLVEQLARRTPGLLLLTATPTQLGLAGHFARLRLLDP
                     NRYDDFETFVREAESYGAVAEIAEKIVEGKPLRPKDHATLKRIFNKDPEGLAQHLDAL
                     ANGRSEARAALLKTLLDQHGTGRVVFRNTRAAMSGFPKRKFCPAPLVDNNVALHARIA
                     RELLAEETGADAEIRHSFRDDPRIEWLAAFLKRHRPAKVLLICKSQRKVMAIEAALKE
                     R"
BASE COUNT          183 a          384 c          263 g          179 t
ORIGIN      
        1 atcctttcct tcaacgccgc ctcgatcgcc atcaccttgc gctgcgattt gcagatcagc
       61 aaaaccttcg ccggccggtg ccgcttcaga aacgccgcga gccactcgat ccgcggatcg
      121 tcgcgaaatg aatgacggat ctccgcgtcc gcccccgttt cctccgcgag cagctcccgc
      181 gcgatgcgcg catgcaaggc gacgttgttg tccacgagcg gtgccggaca gaacttccgc
      241 ttcggaaacc cgctcatcgc cgcacgcgtg ttccgaaaca ccacgcggcc cgtgccatgc
      301 tgatcgagca gcgtcttcaa caacgccgcg cgtgcctccg accggccgtt cgccagcgcg
      361 tccagatgtt gcgcgagccc ttcaggatct ttgttgaaaa tgcgcttcaa cgtcgcgtgg
      421 tctttcggac gcagcggctt cccctcgacg attttctccg cgatttccgc caccgcgcca
      481 taactctccg cctcgcgaac gaacgtttca aaatcgtcgt agcgattcgg atcgagcaat
      541 cgcaaccgcg cgaaatgccc cgccagccca agctgcgtcg gcgtcgccgt cagcaacagc
      601 aaacccggcg tccgccgcgc gagttgttcc accagttgat actccgcgct cgcgccctcc
      661 ggcgaccacg cgaggtgatg cgcttcgtcc acgaccacca tgtcccaacc ggcggcgacc
      721 gcctgctcgc gccgccgttc atccgcggcg agaaacccca cgctgcacaa cgcgagctga
      781 ctcgcctcga acggattctc acccgggtcg ctttcctcca gcgccgcaca ccgcgcttcg
      841 tcgtagatgc tgaaccacag gttgaaccga cgcagcaatt cgacgaacca ctggtgcgtc
      901 agcgattccg gcacgaggat caacacccgc cgcgccttcc ccaccgcgag cagtcgctgc
      961 acgatcaagc acgcctcgat cgttttcccc aaaccgactt cgtcggcga
//
LOCUS       DASPLT010000701         1282 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_68659, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000701 DASPLT010000000
VERSION     DASPLT010000701.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1282)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1282)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1282
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_68659"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..181
                     /locus_tag="VEA63_07345"
     CDS             <1..181
                     /locus_tag="VEA63_07345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD83850.1"
                     /translation="IAGPELARRAVRAGNSVVGFGASLEPATERFPASGLRARAAFYS
                     AGAGGTADDIVYSWR"
     gene            184..894
                     /locus_tag="VEA63_07350"
     CDS             184..894
                     /locus_tag="VEA63_07350"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HYD83851.1"
                     /translation="MHGDLYRSRAGGISRSAFTLIELLVVVALIAGLAALFIAGVGSG
                     SKAGALQSAQATMANLIMAARAKAMASGMRVRILVHADTRDVARFRRFIAMQQETSYL
                     SNAWRPAYERVSLPEGICVLPRVTRVPSGFYAGNMAWTKTSVADPLHSSALDMAVVSA
                     AVDRDDEEVWEVIQFTPFGTMSTNAGDVVLATVVRRAPGSFAEGESPVRAEDVKRVRG
                     MAISTYGVPMLLNGRAAF"
     gene            895..>1282
                     /locus_tag="VEA63_07355"
     CDS             895..>1282
                     /locus_tag="VEA63_07355"
                     /inference="COORDINATES: protein motif:HMM:NF019575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HYD83852.1"
                     /translation="MKRRRGAQGFSLIEVVVAVAVFAIAVVSVLLVLPTLARQTSGSA
                     DLLVVQGLPSAIRSEMKRLAAGGFDAFAAGLPVMTPAPTEGYRMVGARDGLRLTSLTA
                     DNGEVSESEHYFLIELWRFSAPPLGFV"
BASE COUNT          205 a          328 c          493 g          256 t
ORIGIN      
        1 gatcgcggga ccggagctcg cgcgccgggc ggtgcgtgcg ggcaatagtg tggtcggttt
       61 tggggcgagc ctcgagccag cgacggagcg ttttccggca agcgggttgc gcgcgcgagc
      121 ggcgttttac tcggcgggcg cgggcggcac ggcggatgac atcgtttaca gctggaggtg
      181 aatatgcacg gagacttgta tcgaagtcgg gccggcggga tttcacgaag cgccttcacg
      241 ttgatcgaac tgttggtggt tgtagcgctg atcgctggtt tggcggcgct gttcattgca
      301 ggcgtgggca gcgggagcaa agcgggagcg ttgcaatcgg cgcaggcaac gatggcgaac
      361 ttgatcatgg ccgcgcgggc aaaggcgatg gcgagcggaa tgcgggtgcg aatcctcgtg
      421 cacgccgata cgcgcgacgt ggcacgtttt cgacggttca tcgcgatgca gcaggaaacg
      481 agttatttga gcaatgcgtg gcgtccggct tacgagcgag tgagcctgcc ggagggcatt
      541 tgcgttttgc cgcgcgtcac gcgcgtgccg tcgggcttct acgcgggaaa catggcttgg
      601 acaaaaacgt cggtggccga tccgcttcac tcgtcggcgc tcgacatggc cgtcgtgagc
      661 gcggcagtcg atcgcgacga cgaggaggtg tgggaggtga ttcagttcac gccgttcggc
      721 acgatgtcga ccaacgccgg cgacgtcgtg ctcgcgacgg tggtgcgtcg ggcgccaggt
      781 tcatttgcgg agggcgaatc gcccgtgaga gcggaagatg tcaagcgggt gaggggaatg
      841 gcgatcagca cttacggcgt gccgatgctg ctcaacggtc gcgctgcatt ttgaatgaaa
      901 agacgacgcg gagcgcaggg attttcgctg atcgaggtgg tcgtggccgt ggcggtcttt
      961 gcgatcgcgg tcgtgagcgt tttgctcgtg ctcccgacgc tggcgcgcca gacgtcgggg
     1021 tcggcggact tgctcgtggt gcaaggcttg ccgagcgcga ttcgcagcga gatgaagcgt
     1081 cttgccgcgg gcgggtttga tgcgtttgcg gcggggctgc cagtgatgac gccggcgccg
     1141 acggaaggtt atcggatggt tggcgcgcgt gacggcttgc ggctcacgtc gttgacggcc
     1201 gacaacggcg aagtgtccga gagcgagcat tattttctca tcgaattatg gcggttcagc
     1261 gcgccaccgt tgggtttcgt cg
//
LOCUS       DASPLT010000702         1062 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_32301, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000702 DASPLT010000000
VERSION     DASPLT010000702.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1062)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1062)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1062
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_32301"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1062)
                     /locus_tag="VEA63_07360"
     CDS             complement(<1..>1062)
                     /locus_tag="VEA63_07360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009509301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HYD83853.1"
                     /translation="GQTPDSATNSRAVPIYQTTSYVFRDTDHAANLFALKELGNIYTR
                     IMNPTTDVFEQRIAALEGGTAALGHASGQAAITSTILNLAGAGDHIVSVAQLYGGTYN
                     LFHHTLPKLGIEITFVDADDREAFRRALRPNTKAFYGEGLGNPALNIFPVEDVAAIAR
                     SAGVPLILDNTCLSPILHRSFEWGANIVVHSTTKYLGGHGTSIGGVVVDGGNFDWGSG
                     RFPGFTTADASYHGLVHWDAFKAFPPAGGANIAFAMKMRLQLLRDTGNCISPFNAWQT
                     LIGIETLHLRMERICANALAAAQFLQGHPGVAWVNYPGLPSSKYHAAANKYLTGGYGG
                     LLGFGVKSGFEGAKRVINA"
BASE COUNT          203 a          330 c          338 g          191 t
ORIGIN      
        1 acgcgttgat tacgcgcttc gcgccttcga agccgctctt gacgccgaaa ccaagcaggc
       61 cgccgtatcc gccagtcaga tacttgttgg ccgcggcgtg atatttcgac gacggcagac
      121 ccggatagtt gacccacgcg acgccaggat gtccttgcaa gaactgcgcg gcggcgagcg
      181 cgttcgcgca gatgcgctcc atgcgtagat gcaacgtctc gatgccgatg agcgtctgcc
      241 acgcgttgaa cggcgagatg cagttgccgg tgtcgcgcag gagttggagt cgcatcttca
      301 tcgcgaaggc gatgttcgcg ccgcccgcgg gcggaaacgc cttgaacgcg tcccagtgga
      361 ccagtccgtg ataagacgca tccgccgtcg tgaaccccgg aaagcggccc gaaccccagt
      421 cgaaattgcc gccatcgacg accacgccgc cgatcgacgt gccgtgtccg ccgaggtatt
      481 tcgtggtcga gtgaacgacg atgttcgcac cccactcgaa cgaacgatgc agaatcggcg
      541 agaggcaggt gttgtccaga atcagcggca cgcccgccga gcgcgcgatc gcggccacgt
      601 cttcgacggg gaaaatattg agcgccggat tgccgagtcc ttcgccgtaa aacgccttgg
      661 tgttcggccg cagcgcgcga cgaaacgcct cgcgatcgtc ggcatcgacg aacgtgatct
      721 caatgccgag cttcggcagc gtgtgatgaa acaaattata ggtgccgccg taaagttgcg
      781 cgaccgacac gatgtgatcg cccgcgcccg cgaggtttaa aattgtgctc gtgatcgccg
      841 cctggcccga ggcatgcccc agcgccgcgg tgccgccttc gagcgcggcg atgcgctgct
      901 cgaacacgtc cgtcgtggga ttcatgatac gcgtgtagat gttcccgagt tccttcagcg
      961 cgaaaagatt cgccgcgtga tcggtgtcgc ggaacacgta actcgtcgtc tggtaaatcg
     1021 gaaccgcgcg cgagttcgtg gcggagtcgg gcgtctgacc cg
//
LOCUS       DASPLT010000703         1124 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_110242, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000703 DASPLT010000000
VERSION     DASPLT010000703.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1124)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1124)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1124
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_110242"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1124
                     /locus_tag="VEA63_07365"
     CDS             <1..>1124
                     /locus_tag="VEA63_07365"
                     /inference="COORDINATES: protein motif:HMM:NF012931.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="family 20 glycosylhydrolase"
                     /protein_id="HYD83854.1"
                     /translation="LRSAVARTVRRWEERTGFTFARSPQAAFVFATDASAASLVIDCT
                     AAGSALPTLGEDESYTLDASATQIVLRAPNVVGALRGLETLLQLLQRDTDGWFVPKIS
                     IHDQPRFPWRGLMIDVCRHWQPMDVIKRNLDGMALVKLNVLHLHLTEDQGFRIESKTH
                     PRLHELGSDGKYFTQDEIREIIAYAAERGIRVVPEFDIPGHATSWLVAYPELGSAPGP
                     YTIERKWGIFDPVLDPTNEDVYVLLADFLGEMAELFPDPYLHIGGDENNGKHWNANAR
                     IQNFIREQNLHDNEGIHAYFNRRIRDILAPHGKKIVGWDEILHPDIPQDAVIHSWRGP
                     AGLVDAAKRGYSAILSNGYYIDLCYPAADHYRNDPLPADT"
BASE COUNT          211 a          431 c          288 g          194 t
ORIGIN      
        1 gtctccgctc cgccgtcgcc cgcacggtcc gccgctggga ggaacgcacc ggcttcactt
       61 tcgcgcgttc gccgcaggct gcgtttgttt tcgccaccga tgcgtccgct gcttcgctcg
      121 tcatcgattg caccgccgcc ggctccgcgc tgcctacgct cggcgaagac gaatcctaca
      181 cgctcgacgc cagcgccacg cagatcgtcc tgcgcgcgcc caacgtcgtc ggtgcgctgc
      241 gcggcctcga aaccttgctc cagcttctcc aacgcgacac cgacggctgg tttgtcccca
      301 agatttcgat acacgaccag ccgcgcttcc cgtggcgcgg cctgatgatc gacgtctgcc
      361 gccattggca gccgatggac gtgatcaagc gcaacctcga cggcatggcc ctcgtgaaac
      421 tcaacgtcct gcatctccac ctcaccgagg accaaggctt ccgcatcgaa agcaaaaccc
      481 acccgcgcct ccacgagctc ggctccgatg gaaagtattt cacgcaggac gagatccgcg
      541 agatcatcgc ctacgccgcc gaacgcggca tccgcgtcgt ccccgagttc gacatccccg
      601 gccacgccac gagctggctc gtcgcttatc cagaactcgg cagcgcgccc ggcccttaca
      661 ccatcgagcg caagtggggc atcttcgatc ccgtcctcga tccgaccaac gaagacgttt
      721 acgtcctgct cgccgacttc ctcggcgaaa tggccgagct gtttcccgat ccgtatctcc
      781 acatcggcgg cgacgagaac aacggcaagc actggaacgc caacgcgcgt atccaaaatt
      841 ttatacgcga gcagaacctg cacgacaacg aaggcatcca cgcttacttc aaccgccgca
      901 tccgcgacat cctcgcgccg cacggcaaaa agatcgtcgg ctgggacgag attctccacc
      961 ccgatattcc gcaggacgcc gtcatccatt cctggcgcgg ccccgccggc ctcgtcgacg
     1021 cggcgaaacg cggctactct gccattctct ccaacggcta ctacatcgac ctctgctatc
     1081 ccgccgccga tcactatcgc aacgacccgc tgcctgcgga caca
//
LOCUS       DASPLT010000704         1198 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_233472, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000704 DASPLT010000000
VERSION     DASPLT010000704.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1198)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1198)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1198
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_233472"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1198)
                     /locus_tag="VEA63_07370"
     CDS             complement(<1..>1198)
                     /locus_tag="VEA63_07370"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: protein motif:HMM:NF002730.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HYD83855.1"
                     /translation="GRGESTTDLAWDGQIVAYEMGEKIADGERFAREPKLVIADIDVE
                     RVAQERRRLSNFRDAAAQEKHRTASFQRINFELGLAHEKAPLQRALDRFPFVPDDPAK
                     RDHDCYEAYNIQVSGLAKRIETTRSKRVMIGVSGGLDSTHALLVIARAFDLLDLPRKS
                     IVGVTMPGFATSDTTKSNAWALMTALGIDAREVSIAPLAQRMLEDLNHPAARGEPVYD
                     VAYENVQAGLRTDYLFRLANTEGGFVVGTGDLSELALGWCTYGVGDHMSHYSVNASVP
                     KTLIAHLVRWVAETGQIGGAGSDVLVSVLDTDISPELVPGKSGDAPEQKTESVIGPYE
                     LQDFNLYYTLRFGFSPSKVAFLAFHAWRDRTAGRWPDEAHAVKNEYGLAIIKRNLAIF
                     LRRFFQT"
BASE COUNT          198 a          383 c          398 g          219 t
ORIGIN      
        1 tggtctggaa gaagcggcgc aggaagatgg ccaggttgcg cttgattatt gcaagaccat
       61 attcgttctt gacggcgtgc gcctcgtccg gccagcgccc ggcggtccgg tcgcgccacg
      121 cgtggaacgc gaggaacgcg accttcgacg gggagaatcc gaaacgcagc gtgtagtaca
      181 ggttgaagtc ctgcagctcg tacggtccga tcacgctctc ggtcttttgc tccggcgcgt
      241 cgccggactt gccgggcacc agttccgggc tgatgtcggt gtccagcacg ctgaccagga
      301 cgtcggagcc ggccccgccg atctgcccgg tttccgcgac ccagcgcacc aggtgcgcga
      361 tcagcgtctt gggaacgctc gcgttgacgc tgtaatgcga catgtgatcg ccgacgccgt
      421 acgtgcacca gccgagcgcg agttcggaaa gatcgccggt gccgacgacg aagccgcctt
      481 cggtgttggc gaggcggaag agataatcgg tgcgcaagcc cgcctgcacg ttctcgtacg
      541 ccacgtcgta aaccggttcg ccccgcgcgg cggggtggtt caaatcctcc agcatgcgct
      601 gcgccagcgg cgcaatcgaa acttcgcgcg catcgatgcc gagcgccgtc atcagcgccc
      661 aggcgttcga tttggtcgta tcgctggtgg cgaagcccgg catggtgacg ccgacgatgc
      721 tcttgcgcgg cagatccaag agatcgaacg cgcgcgcgat gacgagcagc gcatgcgtcg
      781 aatcgaggcc gccggagacg ccgatcatca cccgcttgct acgcgtcgtc tcgatccgct
      841 tggcgaggcc ggagacctgg atattgtaag cttcgtagca atcgtggtcg cgcttggccg
      901 gatcgtccgg cacgaacgga aagcgatcga gtgcgcgctg cagcggcgct ttctcatggg
      961 cgaggcccaa ttcgaagttg atgcgctgaa agcttgccgt acggtgcttt tcctgcgccg
     1021 ccgcgtcgcg gaaattggag agccgccgcc gctcctgcgc gacgcgctcg acgtcgatat
     1081 cggcgatcac cagtttcggc tcgcgcgcaa agcgctcgcc gtcagcgatt ttctcgccca
     1141 tttcgtaggc gacgatctgg ccgtcccagg cgaggtcggt cgtgctctcg ccgcggcc
//
LOCUS       DASPLT010000705         1245 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_32318, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000705 DASPLT010000000
VERSION     DASPLT010000705.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1245)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1245)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1245
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_32318"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..339
                     /locus_tag="VEA63_07375"
     CDS             <1..339
                     /locus_tag="VEA63_07375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD83856.1"
                     /translation="SWINDVIRRADHPGIGTLPDWGNFSPAEGDRYEEVARMMPTARA
                     VSAKCFDFDAEGRETTLDFQRLVSIIKRSSYRGHLGIEYEGDRLSEADGIKACKTLLE
                     RLQREPMVVA"
     gene            424..720
                     /locus_tag="VEA63_07380"
     CDS             424..720
                     /locus_tag="VEA63_07380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD83857.1"
                     /translation="MLEFVLVLAYGAALIGAAGYAFVNPAGVYGRSAAGVALIIVAAM
                     LLWWLASPMISNEAGFGAFAISAAIVALALLIAITACLAATVRHALNALGVRLL"
     gene            complement(724..>1245)
                     /locus_tag="VEA63_07385"
     CDS             complement(724..>1245)
                     /locus_tag="VEA63_07385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HYD83858.1"
                     /translation="LAPDRIAASVLNDIGPKVNVAGLSRIAAYVGHVQPVASWAEAAE
                     ASKATNGTAFPERSDDDQFWLAFARRTFRARDDGQLELDYDPHIALAFADFDQDAPPP
                     DLTPIFAALAQKPMLSVRGELSDLFGADAVEMMRGLKPDLEAVTIESIGHAPMLDEPE
                     AWDAVLSFLAKAD"
BASE COUNT          206 a          401 c          399 g          239 t
ORIGIN      
        1 agctggatca atgacgtgat acggcgcgcc gatcatccgg gcatcggcac cctccccgac
       61 tggggcaatt tctcgccggc cgagggcgac cggtacgagg aagtggcgcg gatgatgccc
      121 actgcccgcg ccgtcagcgc caagtgcttt gatttcgatg ctgagggccg ggaaaccacg
      181 ctcgatttcc agcgcctcgt ctccattatc aaacgctcga gctatcgcgg ccacctcggc
      241 atcgaatacg aaggcgaccg cctgagcgag gccgacggca taaaggcctg caagaccctg
      301 ctcgaacggc tgcagagaga gccgatggtg gtcgcttagg ccgcgcgggg tgcgccgttc
      361 accattcgca ttaggttcaa tgtttaccac gcagccccat gatcgcttcg cgaaggggcc
      421 ggtatgctcg aattcgttct cgtcttggcg tatggggcgg cgctcattgg cgcggctggc
      481 tacgcgttcg tgaaccccgc gggtgtttac gggcgcagcg cggctggcgt tgcgttgatc
      541 atcgtagctg cgatgctgct gtggtggttg gcctcgccga tgatttccaa cgaagccggc
      601 ttcggcgcct ttgcgatttc ggcggcgatc gtagcccttg ctttgctcat agcgataaca
      661 gcttgcctcg cggctaccgt gcgccacgcg ctcaacgcct tgggcgtgcg tctgctttag
      721 gcgtcagtcc gccttcgcga ggaagctcag caccgcatcc cacgcttccg gttcgtccag
      781 catcggcgcg tggccgatac tctcgatcgt caccgcttca agatcgggct ttaggccgcg
      841 catcatctcg accgcgtccg cgccgaataa atccgacagc tcgccgcgca cggacagcat
      901 cggcttttgc gccagcgcgg cgaatatcgg cgtcaggtcc ggcggcggcg cgtcctggtc
      961 gaaatcggca aaggcgagcg cgatgtgcgg atcgtagtct agttcgagtt ggccgtcgtc
     1021 acgcgcgcgg aaggtgcgcc gggcgaacgc gagccagaat tgatcgtcgt ccgaccgctc
     1081 ggggaacgcg gtcccgttcg tcgccttgct cgcttccgcc gcctcggccc aggacgcgac
     1141 aggctgcacg tgaccgacat aagcggcgat gcgcgaaaga ccggccacgt tcactttcgg
     1201 accgatatcg ttcagcaccg aagcagcgat ccgatcaggc gccag
//
LOCUS       DASPLT010000706         3174 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_151817, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000706 DASPLT010000000
VERSION     DASPLT010000706.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 3174)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3174)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3174
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_151817"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            329..922
                     /locus_tag="VEA63_07390"
     CDS             329..922
                     /locus_tag="VEA63_07390"
                     /inference="COORDINATES: protein motif:HMM:NF014417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006354 - DNA-templated transcription
                     elongation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0140673 - transcription elongation-coupled
                     chromatin remodeling [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination NusG
                     family protein"
                     /protein_id="HYD83859.1"
                     /translation="MDTGITSTRTGWFCLRTQPRREQAAAANLLERVGVEVFAPRIQT
                     RRRSRGGLVTALAEALFPGYLFARFEYPRQIRHVVSTAGVTGIVSFGAAPPPVADEVI
                     EFLRHQIGQAGLGVTTPVLSEGAWVRIAAGCFREIEGRVLSFDPKTERVRVLLSLLGR
                     EVQVSVFAHQVESAAHDAKVFPPGLLASQTDAAPVAR"
     gene            1073..2344
                     /locus_tag="VEA63_07395"
     CDS             1073..2344
                     /locus_tag="VEA63_07395"
                     /inference="COORDINATES: protein motif:HMM:NF021589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HYD83860.1"
                     /translation="MKASQVLLLVTTATLGSSIAHGFAQFARGEALLSTSASARYDSR
                     VFGGVNASDDYIFTLYPQLIYRREAGLLKLDSNLGLRVNRYLDFEEMNSEDLVTSISL
                     RLPPESSTMASGEFETRYDEHTEVNYDVNQRVREKTFVTRLDGALPTSLKTSILGGGS
                     FSHEERNRFSDRETWSGNVGFRYGNFLGGSTFDVRYRRLEVESTGGNAWNIPLNQQSD
                     IYSATLTRPIYHDVRAALSYGYRVLRRSGREVFGQDPRSGGSIISVRINGPFLPESKF
                     PKVDTSITLGYQEAETPGVNDSGGSRFVGAMHVSWQARERTRVFFDARRSLELSINDL
                     TVETTAFEARIGQSIGDFTTASIYAGYEERDYRTVGRQDDAIIGGLSAHYRITKYWSA
                     EGNYRGRLTDSSAFIADYERHIVSLSVNYIF"
     gene            2355..2999
                     /locus_tag="VEA63_07400"
     CDS             2355..2999
                     /locus_tag="VEA63_07400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015774 - polysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HYD83861.1"
                     /translation="MIPLRFAVLPLLAVVAFAQTPPPASLAPAQSLPAANDTDPSYRL
                     APGDSVAVNVYAEPDMSTAQRLDTTGILRLPMIGEIKVGGMSVREAEKALENIYVERE
                     LLKSPLVTIAVSNYALREVSVLGAVRSPGNFQFPKETTSLDIADLITRLGGFSPTAKS
                     DAVSVIRRKPDGTEEVTTVDVERMISGRRRGDSSARREFAVYPGDRIWVPERLF"
BASE COUNT          569 a         1081 c          893 g          631 t
ORIGIN      
        1 ccacgcgaca tacaccgcgg cggcagtgag cacgagagcg cagccgtgga ggcaacgcag
       61 tccttcttgg cgatctttta gcatacggtt ctttccgggt ttatctggct cccgaagcga
      121 cacctgatga tagccggctg ttcgtatcct tggaatcggc gcacgcctgc tctgcccctc
      181 cgctaggctt ccccacccct catcggggat tccctgatca agcgcctcag tgagggaaca
      241 gtcttgcttg aactcacgtc gtgtcgaggg tgcgcgacgt gagtgacgct cgtccaaccg
      301 gacaagtgtc gcacgcagcg cattgcacat ggacaccggc atcacatcga cgcgaacagg
      361 ttggttctgc ctgcgcacgc agccgcgccg cgagcaagcc gcagccgcca atctcctcga
      421 acgcgtcggc gtcgaggtgt tcgcgccgcg cattcaaacg cggcggcgct ctcgcggcgg
      481 acttgtcacc gcactcgcgg aagcgctttt ccctggctat ctcttcgcgc gcttcgaata
      541 cccgcgacaa atccgccacg tggtcagcac tgcgggtgtg acgggcatcg ttagcttcgg
      601 tgctgcaccg ccgccggtcg ccgacgaagt gattgaattt cttcgccatc agatcggtca
      661 ggccgggctc ggcgtgacga cacccgtgct cagcgaaggc gcatgggtgc ggattgctgc
      721 cgggtgtttc cgcgagatcg agggtcgagt gctttccttc gatccaaaaa ccgaacgcgt
      781 gcgcgtgctg ctcagcctgc tcggccgcga ggttcaagtc agtgtgtttg cccaccaagt
      841 cgagtcggcg gcgcacgacg ccaaagtttt tccccctggt ttgcttgcat cacaaaccga
      901 tgcggcacca gtcgcccgat aatcggtcca tgggggcagt gatctcccgg ccaaggggag
      961 aaaaaaatgc ggcccggtgc gcttcttcca gaggtgcgct cgggcggcag cttggcgcaa
     1021 atggcgattt ggtgggcgtc cagctcgctt cgcccaactc ctaactacaa taatgaaagc
     1081 atcccaagtc ctcctgctgg tcaccaccgc cacgctcggt tcatcgatcg cccatggctt
     1141 tgcgcagttc gcccgcggcg aagcgctgtt atcgacgtcc gcctccgcgc gctacgactc
     1201 gcgcgtgttc ggcggcgtga atgcctccga cgattatatc ttcacgctct atccccaact
     1261 gatctaccgg cgcgaagcgg gcctgctgaa attggatagc aatctcggtc tgcgcgttaa
     1321 tcgctacctc gacttcgagg aaatgaactc ggaggacctc gtcacttcca tttcgcttcg
     1381 ccttccgccg gaaagttcga cgatggcgag cggcgagttc gaaacccgct acgacgagca
     1441 cacggaggtc aactacgatg tgaaccagcg cgtgcgcgaa aaaactttcg tcacgcggct
     1501 cgatggcgcg ctgcccacga gcttgaaaac cagcatcctc ggcggcggat cgttcagcca
     1561 cgaagaacgc aatcgtttca gcgatcgcga aacgtggtcc ggcaatgtcg gctttcgcta
     1621 cggtaacttt cttggcgggt ccacgttcga cgtgcgttac cgccgcctcg aggtcgagtc
     1681 caccggcggc aatgcttgga acattccgct gaatcaacag agcgacatct attccgcgac
     1741 gctcacccgc cccatctatc acgacgtccg cgccgccctc agttacggct atcgcgttct
     1801 ccgccgctcg ggtcgcgagg tgttcggcca ggatccgcgc tccggcggtt cgatcatctc
     1861 ggtccgcatc aacggcccct tccttcccga gtcgaagttc cccaaggtcg acacctcgat
     1921 cacgctcggt tatcaggagg ctgaaacacc cggcgtgaac gacagcggcg gcagccgctt
     1981 cgtgggcgcg atgcatgtct cgtggcaggc ccgcgaacgc acacgggttt tcttcgatgc
     2041 gcgtcgctcg ctcgaacttt cgatcaacga tctcaccgtc gagaccaccg cgttcgaagc
     2101 tcgcatcggc cagtcgatcg gcgacttcac caccgcgtcc atctacgcag gatacgagga
     2161 acgtgattac cgcaccgtcg ggcgccagga cgatgcgatc atcggtgggt tgagcgctca
     2221 ctatcgcatc acaaaatact ggtccgccga gggtaattac cgtggccgtc tcaccgactc
     2281 gagcgcgttc atcgcggatt acgaacggca catcgtgagc ctttcggtga actacatttt
     2341 ctaatcgcct ccacatgatt ccacttcgct tcgccgttct gccgctgttg gcggtcgtcg
     2401 ccttcgccca aactccgccg cccgcttcgc tggcgcccgc gcaatccctc cccgccgcga
     2461 acgacaccga cccgtcgtat cggctcgcgc ccggcgattc cgttgccgtc aacgtctacg
     2521 ccgagcccga catgtcgacc gcgcagcggc tcgacaccac cggcatcctt cgcctcccga
     2581 tgatcggaga gatcaaggtc ggcggcatgt ccgtgcgcga agccgagaag gcgctcgaga
     2641 acatctacgt tgaacgcgag ctccttaaat ccccgctcgt caccatcgcg gtcagcaact
     2701 acgcgcttcg cgaagtgtcc gttctcggcg ccgtgcgttc gcccgggaat tttcagttcc
     2761 caaaagaaac gaccagcctc gacatcgcag atctgatcac acggctcggc ggattttctc
     2821 cgacggcgaa atccgacgcc gtgagcgtca ttcgccgcaa accggacggc accgaggagg
     2881 tcactacagt cgatgtcgaa cgcatgatct ccggccgccg ccgcggcgat tcttccgccc
     2941 gtcgcgagtt cgccgtttat cccggcgacc gcatctgggt tcccgagcga ctgttctgat
     3001 caacgttcaa cttcaatttc acgtcacatg cctacaccga agacttccgc cggcgccagc
     3061 tcgcctgcac tccgcatctc gccccgcgac atcctccaga tgttccagga gcgctggctt
     3121 atcgggctcc tcgtaggcgc cgccgccgcg acggcgttcg tgctccttca gccc
//
LOCUS       DASPLT010000707         1648 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_68713, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000707 DASPLT010000000
VERSION     DASPLT010000707.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1648)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1648)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1648
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_68713"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(217..1479)
                     /locus_tag="VEA63_07405"
     CDS             complement(217..1479)
                     /locus_tag="VEA63_07405"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HYD83862.1"
                     /translation="MNPLRLSRLALLLFAGPLLAADLPKPDPDNGGLTLPPGFSAVVV
                     ADKLKPLRNLTVAPNGDLYVKTRQAGIYALRDTDADGRADEINEFGTGGGTGIALHNG
                     YLYHSTDDHVFRYKLTRGELVPTGEPERIVRDLPNKRQHSAKVFAFGADGQLYVEVGS
                     PSNALGQPDRALGAKGQDPTEFLKTHGGYWRFDPDKPDQTQADAHHVSTGHRHSTTIA
                     LNPVSKKLFIALHGRDQLNTVAPQFFTEEDNAENPAEVFHVLRDGADFGWPTTYYDPR
                     TKQRKLNPEYGGDGKKLDESGKFDAPLIAFPAHWAPMQLAFYAGQQFPAKYRNGAFLA
                     FQGSWNRAPKPQQGYKVVFIPFDEKGMPVGTYEPFADNFAGASEIASPGDAKHRPNGV
                     AVGPDGSLYISDSSAGRIWRIIYTGAAN"
BASE COUNT          271 a          395 c          665 g          317 t
ORIGIN      
        1 cgacgaaaag gaagggagag cgcggcagcg aagcgaatgt tcccggtgct ccctgcgctc
       61 acgcgcagag ccaccaagaa tgagcgatgg ccgagcgaga gggcgacgcg gctgcgggcg
      121 ggggttcatc cccgcgctca cgcgcagggc tacggatcgg atggcaccga ccagaggtcg
      181 ggagcctacg gcgctgccgt gccaggttga tgagcgttag ttcgcggcgc cggtgtagat
      241 gatgcgccag atgcggccgg cggaggaatc cgagatatag agcgatccgt cggggccgac
      301 ggcgacgccg ttcgggcggt gcttcgcgtc gccgggcgag gcgatttcgg aggcgccggc
      361 gaagttgtcg gcgaagggtt catacgtgcc gacgggcatg cccttttcgt cgaagggaat
      421 gaagacgacc ttgtaacctt gctgcggttt tggcgcgcga ttccacgaac cttggaacgc
      481 gagaaaagcg ccgttgcgat acttcgcggg gaattgctgg ccggcgtaga acgcgagctg
      541 catgggcgcc cagtgcgcgg ggaaagcgat cagcggcgcg tcgaacttgc cggattcgtc
      601 gagtttctta ccgtccccgc cgtattcggg gttgagttta cgttgtttcg ttcgcgggtc
      661 gtagtaggtg gtgggccagc caaagtcggc gccgtcgcgg agcacgtgga agacttcggc
      721 ggggttttcg gcgttgtctt cttcggtgaa gaactgcggc gcgacggtat tcaactggtc
      781 gcgaccgtga agcgcgatga agagtttttt ggaaacgggg ttgagcgcga tggtggtcga
      841 gtggcggtgg ccggtggaaa catggtgggc gtcggcctgg gtttggtcgg gcttgtcggg
      901 atcgaagcgc cagtaaccgc cgtgcgtttt gaggaattcg gtggggtcct ggcctttcgc
      961 gccgagtgcg cgatcgggtt ggccgagggc attggagggc gagccgactt cgacgtagag
     1021 ctgaccgtcg gcgccgaagg cgaaaacttt tgcggagtgc tgacgtttgt tggggagatc
     1081 gcggacgatg cgctcgggtt cgccggtggg aacgagttcg ccgcgcgtga gtttgtagcg
     1141 gaaaacgtga tcgtcggtgg agtggtagag atagccgtta tggagggcga tgccggtgcc
     1201 gccgccggtg ccgaactcgt tgatctcgtc ggcccggccg tcggcgtcgg tgtcgcgcag
     1261 ggcgtagatg ccggcctggc gggttttgac gtagaggtcg ccgttgggcg caacggtgag
     1321 gttgcgaagg ggtttgagtt tatcggcgac gacgacggcg ctgaagccgg gcgggagggt
     1381 gaggccgccg ttgtcgggat cgggtttcgg caggtcggcg gcgaggagcg ggccggcgaa
     1441 gagcaacagc gcaaggcgag aaagtctgag ggggttcatg cgcgcggtat gacggacgag
     1501 gcgcggcggg gattcgcggt ggaggaaagt tgagcgagcg gagtttgtca tggagaggcg
     1561 agtgcgacgg cggaggattg gttttgatcg ttctcgttct cttaatcgtt ctcgttctcg
     1621 cggtcgaaac gatgggggga gggaagtg
//
LOCUS       DASPLT010000708         1588 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_274375, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000708 DASPLT010000000
VERSION     DASPLT010000708.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1588)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1588)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1588
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_274375"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1588
                     /locus_tag="VEA63_07410"
     CDS             <1..>1588
                     /locus_tag="VEA63_07410"
                     /inference="COORDINATES: protein motif:HMM:NF028442.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HYD83863.1"
                     /translation="SEAALHRGARVNRWEEKAGFSFLFEYGSVPTPDVSADATVPAGE
                     VIDLTARLQRDGTLEWDAPPGNWTVLRLGYSLTGAKNRPATPAGSGYEADKLSRAHME
                     AYYHGYFDPIEKALGPHFGKSLRYVMMDSWEAGTNNWTDNLAAEFRQRRGYDPMPWLP
                     ALTGRIVGSADMSDRFLWDFRRTLADLWADAHYGTMADKLRERGIGIYAEAAGVSLEM
                     PEDTLLNKSKVEIPMGEFWVNDLHPRLMYLQDVRGAASAAHAYGKPLVAAEAFTGGGY
                     ESPAALKNAADYWFAQGVNRLVFHTSAHQPLDTKPGNTMVGTHLNRNITWAEQAKPLF
                     TYFARASHLLQQGLFVADLAYLLDEGAPSTPPIWGAGTRPSPPDGYDYDFINADVLLN
                     RLSVADEGQLLLPDGMSYRVLVLPETQRMRPELLRKLRELVAGGATIVGPKPTHSPSL
                     ANHPEADEEVRALAADLWGDLDGASRTVRHYGKGRVVWGLPSSDVLTALQIPKDFDYV
                     RGLDADMAWLHRRTPTADIYY"
BASE COUNT          289 a          523 c          504 g          272 t
ORIGIN      
        1 gagtgaggcg gcgctgcacc gcggcgcgcg cgttaatcgc tgggaggaga aggctggctt
       61 cagcttcctg ttcgaatacg gcagcgtgcc gacccctgac gtttccgccg acgcgaccgt
      121 tccggccggc gaagtgattg acctcaccgc tcgcctgcag cgcgacggca cgcttgagtg
      181 ggatgcgccg ccggggaact ggacggtgct gcgcctcggc tactcgttga ccggtgcgaa
      241 aaaccgtccg gccacacccg ccggctccgg ctacgaagcc gacaagctga gccgtgcgca
      301 catggaggct tattaccacg gctatttcga tccgatcgaa aaagcgctcg gtccgcactt
      361 cggaaaaagt ctgcgctacg tgatgatgga cagttgggaa gcgggcacga ataactggac
      421 cgacaacctt gccgccgaat tccgccagcg ccgcggctac gatccgatgc cgtggttgcc
      481 cgcgctcacc ggtcgcatcg tcggcagcgc ggatatgagc gaccgctttc tctgggattt
      541 ccggcgaacg ctcgcggacc tgtgggccga cgcgcattac ggcacgatgg ccgataagct
      601 gcgcgagcgc gggatcggca tttacgccga agccgccggc gtctcgctcg aaatgccgga
      661 agacacgctg ctcaacaaaa gcaaggtcga gattccgatg ggcgagtttt gggtgaacga
      721 tctccatccg cgtctgatgt atctgcagga cgttcgcggg gccgcgtcgg ccgcgcacgc
      781 ttacggcaaa ccgctggtcg ctgcggaagc attcaccggc ggcggctacg aatcgcccgc
      841 ggcgctcaaa aacgccgccg actattggtt cgcgcagggc gtcaaccgcc tcgtcttcca
      901 cacgtccgcg catcagccgc tcgatacgaa acccggcaat acgatggtgg gcactcacct
      961 gaatcgaaac atcacgtggg ccgaacaggc gaaaccgctc ttcacgtatt tcgcccgcgc
     1021 gagccacctg ttgcagcaag ggctgttcgt cgcggatctc gcttacttgc tcgacgaagg
     1081 cgcgccctcg accccgccga tctggggcgc cggcacgcgt ccgtctccgc cagatggata
     1141 cgactacgat ttcatcaacg ccgacgtgtt gctgaatcga ctgagcgtcg cggacgaggg
     1201 gcaattgctg ctgccggacg gcatgagcta tcgcgtgctt gtgttgcccg aaacccagcg
     1261 gatgcgaccg gagctcctgc gcaagctgcg cgaactcgtc gccggcggcg ctacgattgt
     1321 cggcccgaag ccgacgcact cgccgagtct cgccaaccat cccgaggctg acgaagaagt
     1381 gcgcgcgctg gcggccgatc tctggggcga tctcgacggc gcgagccgca ccgtgcgcca
     1441 ctacggaaaa ggtcgcgttg tgtgggggct gccctcgagc gacgttctca ccgcgctcca
     1501 gatcccgaag gatttcgact acgtccgcgg actcgatgcc gacatggcgt ggttgcaccg
     1561 acgcacgccg accgccgaca tttactat
//
LOCUS       DASPLT010000709         4780 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_274382, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000709 DASPLT010000000
VERSION     DASPLT010000709.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 4780)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4780)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4780
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_274382"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(80..277)
                     /locus_tag="VEA63_07415"
     CDS             complement(80..277)
                     /locus_tag="VEA63_07415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD83864.1"
                     /translation="MAVKVTPFFRWYDLWIGAYVDTKGKTLYICPLPMLGLKIQYGDP
                     RCQCEECLALRRQLADYEVQA"
     gene            complement(268..729)
                     /locus_tag="VEA63_07420"
     CDS             complement(268..729)
                     /locus_tag="VEA63_07420"
                     /inference="COORDINATES: protein motif:HMM:NF024929.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HYD83865.1"
                     /translation="MLVPQSWRSANLRQLIIPTAAGGKIATVHKDAIAVFDAWLALMS
                     ERGIDKDILTYDGGFVPRLKRGVDQPKDRASKAAWGKLLSNHSRGTAIDLNAKWNPMG
                     KYGATNGTEGSLARVIACAREVRVGDLGIVCGADWKGRSRDDMHFEIGVWP"
     gene            complement(980..1156)
                     /locus_tag="VEA63_07425"
     CDS             complement(980..1156)
                     /locus_tag="VEA63_07425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD83866.1"
                     /translation="MISWLVKKHEESIAKKSLERLRSGYALPGDTQRVTKHYGDVGDL
                     IDVNRGRGKRRSDR"
     gene            complement(1323..1913)
                     /locus_tag="VEA63_07430"
     CDS             complement(1323..1913)
                     /locus_tag="VEA63_07430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD83867.1"
                     /translation="MTRILNAIPRENAADYRRVLNKQDLPKLKRKQPVKAYVGQFHKF
                     DPAKSTHTLQLPIAFRTAEYNAGRTPAQLRARWVKEKRELVWRVMHTHMPTFARCIPE
                     GISLRVTHIEFVRIGQRALDDDNLVAAFKAVRDALCAFLVWGHECDENIRQIGRADDI
                     LKQRGVRWSYRQQKCESNPRLYGVRLILHCAPRSSE"
     gene            complement(1910..2206)
                     /locus_tag="VEA63_07435"
     CDS             complement(1910..2206)
                     /locus_tag="VEA63_07435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD83868.1"
                     /translation="MSAAAELRDVLHDARPKTLRPIVRTTVLRAPHLEVGDISTRVES
                     LCGQVDCRRCEQPRQQQLVARTSLGAETWDVLPCQCGQPLITLGDFIRERGLAG"
     gene            complement(2203..2406)
                     /locus_tag="VEA63_07440"
     CDS             complement(2203..2406)
                     /locus_tag="VEA63_07440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD83869.1"
                     /translation="MTGEERDALVLELQDRLGEIEVEMYDLEAERIRIEEREAELRPE
                     DERIRARIWELEDTACEYAEDAS"
     gene            complement(2403..3716)
                     /locus_tag="VEA63_07445"
     CDS             complement(2403..3716)
                     /locus_tag="VEA63_07445"
                     /inference="COORDINATES: protein motif:HMM:NF015736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DnaB-like helicase C-terminal domain-containing
                     protein"
                     /protein_id="HYD83870.1"
                     /translation="MSERIGLPHDHHLERKVLNLALHFAEHRQLLVDELLPSDFYADG
                     TRAVFEGIARLHRDAGQNFDLATLTSASSGSELILEFIAAAYELTMLSLEEYEGAVER
                     LRQLAEARTLARACLDVGACGLESAATHDPNEFLQRASERLTAALAGRRQKRVGRRLG
                     EVLDLAIQEIVAERPVTPRIVAPSGIDPVDRALPWRGFASGELAIVLARPGIGKTSFA
                     LQIARNNAVAGLHTAVFSYEMSELDVGLSVLSQDARIPVQAFARKLSADTATHLVNRA
                     GMLSDIPLYIFDCAGLTLEDLANTCRAMKRKGQLDLVAIDYLQLMRMRKRYQSRFEEI
                     GDIATALKLLSRELDVPFVVLSQSNRDSDKRGDKRPVMSDIAQSGQIERDADRIIGLH
                     RECVYDANADKTAAEAVFLKNRKGDTGIVRMRFDAPTTTFSGELS"
     gene            complement(3741..4577)
                     /locus_tag="VEA63_07450"
     CDS             complement(3741..4577)
                     /locus_tag="VEA63_07450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD83871.1"
                     /translation="MDFANESYVRVYTRDTMTWKRLNWQARCVWSLLLRKLDRAGVLD
                     LEGLQPDEAVALATELPPDVVEVGLPQLFDRGVIEAHDDLLVAPRFLEAQECVKSDKQ
                     RAKEYRERRRSQAMAEPSQNVTPESRDVTQSSREITAVTDRHASVTLNSAVLPSALPG
                     IAVPYAVVTAAEKIGRAWYAELVQKDVASIPSASAAYEFIGKQSADERATVAANLRAS
                     SVHGKKLRALNPKRIVDSLWHSHLLSEQEHAAQKQQYANGRQQLRAAEVVPSAHQPMK
                     LL"
BASE COUNT          737 a         1561 c         1525 g          957 t
ORIGIN      
        1 ccaagcatgg cgcagacggc gagcgcgccc atgattgcgc cggctatgtc gccggccgtg
       61 atgactgctg gcacgttgtt cacgcttgca cctcgtagtc agccagctga cggcggagcg
      121 ccaagcactc ctcgcactgg cagcgcggat cgccgtactg aatcttgagc ccgagcattg
      181 gcagcgggca gatgtagaga gtcttgcctt tggtgtcgac gtacgcgccg atccataggt
      241 cgtaccagcg gaagaatggc gtcaccttca cggccacacc ccgatctcaa agtgcatgtc
      301 gtcgcgcgat ctacccttcc agtccgcgcc gcagacgatg cccaggtcac cgacgcggac
      361 ctctcgcgcg cacgcgatca cgcgcgcgag actgccctcg gtgccgtttg tagcgccgta
      421 cttccccatg gggttccact tcgcgttgag gtcaatcgct gtcccgcgcg agtggttgga
      481 cagcagcttg ccccacgccg cttttgacgc gcggtccttt ggctgatcga caccgcgctt
      541 gagccgcggc acgaagccgc cgtcatacgt caggatgtct ttgtcgatgc cgcgctctga
      601 catgagcgcg agccacgcgt cgaacaccgc gatcgcgtct ttgtgcaccg tcgcgatctt
      661 gccgcctgcc gcggtcggga tgatcagctg acgcaggttc gctgatcgcc acgactgcgg
      721 cacgagcacc cgtccctcgt cgtcgactgt gaagtcgatt ggatagcgcg cgcatcgctc
      781 ctcgtacgtg agctgacgcg gccggtccgc ttgcttagtt gttagttgct tcacttcatt
      841 cgctccggtt tgattccatg cttacgcgcg actgcgcgtg ctctgtgctc gtctgcttcg
      901 ttccagtcct cgccgcgctc gattgcgcgc ttcatgcggc gcgtgtgttt ggcgagccag
      961 aggtcggccc agaatcccat cagcggtcac tccgccgctt gcctctgcct ctattgacgt
     1021 cgatcagatc gccgacgtcg ccgtagtgtt tggtcacacg ctgcgtgtct ccgggcaggg
     1081 cgtagccgct gcgcagacgc tctagcgatt tcttagcgat ggactcttcg tgtttcttta
     1141 cgagccagct gatcatgtgt tttttcctgg ttggttggtt ccgttatcgt tcgatcgctc
     1201 tagccgctgc agcgcagcga ccatgagact tgagaactct ctgcctgcgt tgagcgcgcc
     1261 ggtcccgtaa gcgcgcagcc cgcacgcgtc tagctcgtcg gcgagctcgc gcagcgcgtt
     1321 gatcactcgg acgaacgcgg cgcgcagtgc agaatgagac gtactccgta aagcctcggg
     1381 ttgctctcgc acttctgctg ccggtaactc catctgaccc ctcgctgttt cagaatgtcg
     1441 tctgcacgtc cgatctgcct gatgttctcg tcgcactcgt ggccccatac gaggaacgca
     1501 cacagcgcgt cgcgtacggc tttgaacgcg gcgacgaggt tgtcgtcgtc gagcgctcgc
     1561 tggccgatgc gcacgaactc tatgtgcgtg actcgcaggc tgatgccttc cggtatgcag
     1621 cgagcgaacg tcggcatgtg cgtatgcatc acgcgccaga caagctcgcg cttctctttg
     1681 acccagcgag cgcgcagctg cgccggtgta cgtcctgcgt tgtactccgc ggtgcggaat
     1741 gcgatcggca gctgcagcgt gtgcgtgctc ttggcggggt cgaacttatg gaactgcccg
     1801 acgtacgctt tgaccggctg cttgcgcttc agcttcggca ggtcctgttt attgagcacg
     1861 cgccgatagt cagccgcgtt ctcgcgcggg atcgcgttga ggatgcgagt catcccgcga
     1921 gcccccgctc cctgatgaag tcgccgagcg tgatgagcgg ctggccgcac tgacacggca
     1981 gcacgtccca ggtctcagcc ccgagcgacg tgcgcgctac gagctgctgc tgcctcggct
     2041 gctcgcagcg gcggcaatcc acctgtccgc acaggctctc gacacgcgtg ctgatgtcgc
     2101 cgacttcgag gtgcggtgcg cgcagcacgg tcgtgcgcac gattgggcgt agcgtcttgg
     2161 gccgcgcgtc gtggaggacg tcgcgaagct ctgcggcggc gctcatgacg cgtcctccgc
     2221 gtattcgcaa gccgtgtcct caagctccca aatcctggcc cggattcgct catcctccgg
     2281 ccgcagttct gcctcgcgct cctcgatgcg aatgcgctcg gcttctaggt cgtacatctc
     2341 gacctcgatc tcgccgagcc ggtcctgcag ctcgagcaca agcgcgtcgc gttcctcgcc
     2401 ggtcatgaca gctccccgct aaacgtcgtg gtcggagcgt cgaagcgcat gcgaacgatg
     2461 cccgtgtcgc ccttgcggtt cttgaggaac acggcctcag ccgcagtctt gtccgcgttc
     2521 gcgtcgtaga cgcactcgcg gtgcagcccg atgatgcgat cagcatcgcg ctcgatctga
     2581 ccgctctgcg cgatgtcgct catcacgggg cgcttgtcgc cgcgcttgtc ggagtcgcgg
     2641 ttgctctgcg agagcacgac gaaaggcacg tccagctcgc gcgacaggag cttgagcgcg
     2701 gtcgcgatgt cgccgatctc ctcgaagcgc gactgatagc gcttccgcat gcgcatcagc
     2761 tgcaggtagt cgatcgccac aaggtcaagc tgacccttgc gcttcatggc gcggcacgtg
     2821 ttggcgagat cttcgagcgt caggccggcg cagtcgaaga tgtacagcgg gatgtccgac
     2881 agcatgccgg cgcggttgac caagtgcgtc gcggtgtcgg cgctgagctt gcgcgcgaac
     2941 gcctgcacgg ggatgcgcgc gtcctgcgag agcacgctga ggcccacgtc cagctcgctc
     3001 atctcgtacg agaacaccgc cgtgtgcagg ccggcgaccg cgttgttgcg tgcgatctgc
     3061 agcgcgaacg acgtcttgcc gatgccgggg cgagccagca cgatcgcgag ctcgccggaa
     3121 gcgaagccgc gccaaggcag cgcgcggtcc acaggatcga tcccgctcgg cgcgacgatg
     3181 cgcggcgtga ccggccgctc ggcgacgatc tcctgaatcg ctaggtccag cacttcgcca
     3241 agtcgacgcc ctactcgctt ttgccgccgc cccgctaagg cggctgtgag cctctcagag
     3301 gcgcgttgca gaaactcgtt cgggtcgtgc gttgccgccg actccaggcc gcacgcgcca
     3361 acgtcgaggc aagcgcgcgc caaagtgcga gcctcggcca gctggcggag ccgctcgact
     3421 gcgccctcgt actcttcgag cgagagcatg gtcagctcgt acgcggctgc gatgaactcg
     3481 aggatcagct ccgagccgct cgacgcgctc gtaagtgtgg ccaggtcgaa gttctgcccg
     3541 gcgtcgcggt gcagtcgagc gatgccctcg aacacggcgc gcgtgccgtc ggcgtagaag
     3601 tcgctcggca ggagctcgtc gacaagtagc tgccggtgct cggcgaagtg gagcgcgagg
     3661 ttgagcacct tgcgctcgag gtgatggtcg tgtggcaatc cgattcgttc tgacatttgc
     3721 gtgttcctgg ttggttgagg tcagagcaac ttcatgggtt ggtgcgcgga cgggacgacc
     3781 tcggcagcgc gcagctgctg ccgaccgttc gcgtattgct gtttctgcgc tgcgtgctcc
     3841 tgctcgctga gcaggtgcga gtgccaaagg gagtcgacga tgcgcttcgg gttgagcgcg
     3901 cggagcttct tcccgtgcac cgagctggct cggaggttgg ccgcgaccgt cgcgcgttca
     3961 tcggccgact gcttgccgat gaactcgtag gcggcagacg cggacgggat gctggccacg
     4021 tccttctgga ccagctctgc gtaccaggcc cggccgatct tctccgccgc cgtcaccacc
     4081 gcatacggca ctgcaatgcc aggcaaagca gagggaagca cagcagagtt aagagtgacg
     4141 ctcgcgtgac ggtccgtgac ggccgtgatt tcgcgtgacg attgcgtcac gtctcgtgac
     4201 tccggcgtca cgttttgtga cggctctgcc atagcttggc tacggcgccg ttcccggtac
     4261 tccttcgcgc gttgcttgtc cgacttcacg cactcctgcg cttcgaggaa gcgcggagcc
     4321 acgagcaggt cgtcgtgcgc ctcgatgacg cccctgtcga acagctgcgg cagaccgacc
     4381 tcgacgacat ccggcggcag ctcggtagcg agcgctaccg cttcgtcggg ctggaggccc
     4441 tcaaggtcga ggacccctgc ccggtcaagc ttgcgaagca agagcgacca tacacagcgc
     4501 gcctgccagt tgagccgctt ccaggtcatc gtgtcgcgcg tgtagacgcg gacatagctc
     4561 tcgttcgcaa agtccatgcg atgtagtcac cttgtcatac aaacgtatgc ttgttagatc
     4621 gtgggtgggc gagcgcggca aagccgcacg tcgtggcagt cgggtgcttc accgcgctcg
     4681 gtacgtaagc caccgcacag ctggctgacc tacccagcac gtcgccgctc gcattggcta
     4741 catttcagcc tgcggggcgt tgtgggggaa accactacaa
//
LOCUS       DASPLT010000710         1762 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_233554, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000710 DASPLT010000000
VERSION     DASPLT010000710.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1762)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1762)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1762
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_233554"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(39..1340)
                     /locus_tag="VEA63_07455"
     CDS             complement(39..1340)
                     /locus_tag="VEA63_07455"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HYD83872.1"
                     /translation="MTSSPASPSFALPSAGAGAVWLHVGTLIDGTSTRPCRDAHVVFN
                     RAKILFVGVDGRMPPREVLRAGQTAPDVDAPDATLLPGLIEAHAHLFLEGGELDADKR
                     AAYLQQTPEQLLARARERLPKLVRYGIAGVRDAGDKDGVGLALSKLCASRERPLMPYV
                     ESPGAALHRKGRYGSFMADAVENHSSLRSVVEARVAAGADRIKLIPTGIINFKKAAVT
                     AEPQMTTEDVAQLVEAARAFHRQTFAHASGDVGIDRAIDGGVDSIEHGFFIRDDQLAK
                     LRDKQTAWVPTFAPVQKQVDHAEIMKWDAEIVTNLRGILERHAASLAKAHAMGVLIVA
                     GSDAGSYGVAHGVDFVYELELMERAGVPSIGVINAATGVSAGRLRYKEKIGRIEQGCA
                     SRFILTRHSPLETVANLRKARTVIFDGEVFETDQRADVQGL"
     gene            complement(1481..>1762)
                     /locus_tag="VEA63_07460"
     CDS             complement(1481..>1762)
                     /locus_tag="VEA63_07460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012377132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HYD83873.1"
                     /translation="IVGLSNFAWGTPKGIREQLENAYLTLAMEHGLDYALANPEKSPG
                     PLPKDHPMVDKLREALAQGRAQDGESQETAGFRQAEAIMAICAEAEPVD"
BASE COUNT          331 a          630 c          484 g          317 t
ORIGIN      
        1 catctctgtg cctctgtggt tcaaatgctt agtcgtattt agagcccttg cacatccgcg
       61 cgctgatccg tctcgaacac ctcgccgtcg aagatcaccg ttcgcgcctt gcgcaaattc
      121 gcgaccgtct cgagcggcga atgccgcgtc aggatgaacc ggctcgcgca cccctgctcg
      181 atgcgtccga tcttctcctt gtagcgcaac cgtcccgcgc tcacgcccgt cgccgcattg
      241 atcacgccaa tcgacggcac gcccgcgcgc tccatcaact cgagctcgta aacaaaatcg
      301 acgccatgcg ccacgccgta agagccggcg tcgctgcccg cgacgatcaa cacgcccatc
      361 gcgtgcgcct tcgccaaact tgccgcgtga cgttcgagaa tcccgcgcag attcgtcacg
      421 atctccgcgt cccacttcat gatctccgcg tgatcgacct gcttttggac gggcgcaaac
      481 gtcggcaccc acgcggtctg cttatcgcgc aactttgcga gctggtcgtc gcgaataaaa
      541 aacccgtgct cgatcgaatc gacgcccccg tcgatcgcgc gatcgatccc cacgtcgccc
      601 gacgcatgcg caaacgtttg ccggtgaaac gcgcgcgccg cttcgacgag ttgcgccaca
      661 tcttcggtcg tcatctgcgg ctccgccgtc accgccgcct ttttgaaatt aatgatgccc
      721 gtcgggatca gcttgatgcg atccgcaccc gccgcgaccc gcgcctccac caccgaccgc
      781 aacgacgaat ggttttccac cgcatccgcc atgaagctcc cgtagcgccc cttgcgatga
      841 agcgccgcgc cgggactctc gacgtaaggc atgagcggcc gctcgcggct cgcacaaagt
      901 ttgctcaacg cgagtccgac gccgtccttg tcacccgcat cgcgcacgcc cgcgatgccg
      961 tagcgcacca gcttcggcaa acgctcgcgc gctcgcgcca acaattgctc gggcgtttgt
     1021 tgaagatacg ccgcgcgctt gtccgcatcg agttcgccgc cttcgagaaa gagatgcgcg
     1081 tgcgcctcga tcaaacccgg cagcagcgtc gcatccggcg catcgacgtc cggtgcggtt
     1141 tgccccgcgc gcaacacttc gcgcggcggc atccggccgt ccacgccgac aaataaaatc
     1201 ttcgcgcgat taaaaaccac gtgcgcatcg cgacacggcc gcgtgctcgt gccatcaatc
     1261 aacgtgccaa cgtgcaacca gacggcgccg gcgccggcgg atggaagagc gaacgaagga
     1321 gaagcgggcg atgacgtcat gtgattcggg cgccggaagc tccaagctcc aagcgccaag
     1381 gaccaaagaa acaacaaacc gcaaaaccca attggagttt gaacattgaa aattctctgg
     1441 agcttggagg ttggagcttg gagcttccga cgttagtcgg ctagtcgacc ggttccgcct
     1501 ccgcgcagat cgccatgatc gcctccgcct ggcggaagcc cgccgtctcc tgcgactcgc
     1561 cgtcctgcgc gcggccctgc gccagcgcct cgcgcaactt gtcgaccatc ggatgatcct
     1621 tcggcagcgg accgggcgac ttttccggat tcgcgagcgc atagtccaag ccgtgctcca
     1681 tcgcgagggt gaggtaagcg ttctccagct gctcccgaat tcccttcggc gtgccccacg
     1741 cgaaattcga caagcccacg at
//
LOCUS       DASPLT010000711         1738 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_32383, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000711 DASPLT010000000
VERSION     DASPLT010000711.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1738)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1738)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1738
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_32383"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..786
                     /locus_tag="VEA63_07465"
     CDS             <1..786
                     /locus_tag="VEA63_07465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD83874.1"
                     /translation="TGAAAAITADFSADPALNQITRMSFIDLDNDIVQVELSGPGTLS
                     LVLDGATPPALPTRYKQEINYMKGHAGIVIVGADESTHVSVFSVGRATAYDPTGAFNF
                     LQPIDPITNNPDDNRNPLFEGRTAADYDGIADIAFIAISSPTGKFGGVRTANTTYFAD
                     HGLTGIYAPGIAFSGPVYIGDITAFDDAEPVIILGSAADTRITGGSLDQPNDHPVEVG
                     GVTQLIFTPGTDSHGRFLPAKTNQASLRQNSQDVTAQIVVNPQ"
     gene            912..>1738
                     /locus_tag="VEA63_07470"
     CDS             912..>1738
                     /locus_tag="VEA63_07470"
                     /inference="COORDINATES: protein motif:HMM:NF012415.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HYD83875.1"
                     /translation="MHLVSRLVFLLLCTTPLWAQIPYSIDGTSGPPTDEETFYLELIN
                     RARANPTAEGERLRTTTDTTVLAAVDFFDTDLNLMAQEMSALPPAPPIAFNAKLTNAA
                     RAHSLDQFTHQFQGHVGSNGSDGVSRVTAAGYSFQTFGENVYATASSVFYGHAGFEID
                     WGNNDIGGMQAGRGHRLIIHKPDIREVGIGVVLGTNGTVGPQVVTQDFGTQLNATPLL
                     TGVAYYDLSGDNFYTPGEGLGGINVTVSNATFSAVTSPSGAFTVPLPGDGIYNVRFSG
                     "
BASE COUNT          341 a          689 c          406 g          302 t
ORIGIN      
        1 accggcgccg ccgccgcgat caccgccgac ttttccgccg accccgcgct caatcaaatc
       61 acgcgcatgt ctttcatcga tctcgataac gacatcgttc aagtcgaact cagcggcccc
      121 ggcacgctct cgctcgtcct cgacggcgca acaccgcccg cgctccccac acgctataaa
      181 caggagatca actacatgaa aggtcacgcc ggcatcgtga tcgtcggcgc cgacgaaagc
      241 acccacgtct ccgtcttcag cgtcggccgc gccaccgctt acgatcccac cggcgcgttc
      301 aatttccttc aaccgatcga tcccatcacc aacaatcccg acgacaaccg caacccgctc
      361 ttcgaaggcc gaactgcggc cgactacgac ggcatcgccg atatcgcttt catcgcgatc
      421 tcgagcccca ccggaaaatt cggcggcgtg cgcaccgcca acaccaccta cttcgccgac
      481 cacggcctca ccggcatcta cgcgcccggc atcgcgttct ccgggcccgt ctacatcggc
      541 gacatcaccg ccttcgacga cgccgaaccc gtgatcatcc tcggctccgc cgccgacacc
      601 cgcatcacgg gcggcagtct cgatcagccc aacgaccacc cggtcgaagt cggcggtgtg
      661 acccagctaa tattcacccc cggcaccgat tcccatggcc gcttccttcc cgccaaaacc
      721 aaccaagcct ccctccgcca aaacagccaa gacgtgaccg cccaaatcgt cgtgaatccg
      781 caataaccgg tgaaacccga cgtgccgtcg ggttgttcgc gtccgtcctc gttcgaggga
      841 cacaagaatc taagggaaac tcctcagcat gcttgccctt acgccgccgc ctcccttgcc
      901 tcggcgcatt catgcatctc gtctcacgcc tcgtcttcct gcttctgtgc accacgccgc
      961 tttgggctca gattccttac tcgatcgacg gcacgagcgg cccgcccacc gacgaagaaa
     1021 cattttatct cgagctgatt aaccgcgccc gcgccaaccc caccgccgag ggcgagcgac
     1081 ttcgcacgac gaccgacacc accgtcctcg ccgccgtcga tttcttcgac acggacctga
     1141 atctcatggc gcaagagatg agcgctcttc cccccgcgcc tccgatcgcc ttcaacgcca
     1201 agctcaccaa cgccgcccgc gctcactccc tcgatcagtt cacgcatcaa ttccaaggtc
     1261 acgtaggctc caacggcagc gacggcgtgt cccgcgtcac cgcggccggc tacagttttc
     1321 aaacattcgg tgaaaacgtc tacgccaccg cctcctccgt cttctacggc cacgccggtt
     1381 tcgaaatcga ctggggcaac aacgatatcg gcggcatgca agccggccgc ggtcaccgcc
     1441 tcatcatcca caaacccgac atccgcgaag tcggcatcgg tgtcgtgctt ggcacgaacg
     1501 gcaccgtcgg cccccaagtc gtcacgcagg atttcggcac gcaactcaac gccaccccgc
     1561 tcctcaccgg cgtcgcctat tacgatcttt ccggcgacaa cttctacacg cccggcgaag
     1621 gtctcggcgg catcaacgtc acggtttcca acgccacctt ctccgccgtc acctcgccgt
     1681 caggcgcgtt caccgtgcca ctgccgggcg atggcattta caacgtccgt ttcagcgg
//
LOCUS       DASPLT010000712         1159 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_9189, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000712 DASPLT010000000
VERSION     DASPLT010000712.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1159)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1159)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1159
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9189"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(183..>1159)
                     /locus_tag="VEA63_07475"
     CDS             complement(183..>1159)
                     /locus_tag="VEA63_07475"
                     /inference="COORDINATES: protein motif:HMM:NF012703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="arginase family protein"
                     /protein_id="HYD83876.1"
                     /translation="FELGIGRISVPTGQWLGGGALGRSRLSSNSTMQPQPVILLAPSV
                     LGLDGTGVNQLGDALLEAGLAARLNAPVIARVDAPPQNPVRHPEHGMLNSDTLAGYTV
                     TLADAVEPILARGEFPLVLGGDCTILLGNLLALRRRGRFGLLFIDAHADFYQPEANVN
                     GEGASSELALATGRGPHGLTRFEHHERLIDDEDVVVFGFRDLDEQRHFGSQPLAPGIH
                     AMDLAEVRRLGVEHALGRALAHLTRAELAGFWIHFDADVLDDAIMPAVKYHLPDGFSW
                     DEIVSILRTALACDRAVGLEVTIYDPTLDPSRRAARGLVDAIARGFTR"
BASE COUNT          228 a          364 c          380 g          187 t
ORIGIN      
        1 ctaaaaccag caggtaagtc ggcgtagagc gcagaacaag gaaagcgatt ttggcgattt
       61 tcggaaacgc tggtgcgccg cgtttggagt cctcgcgctt agcccgtcat ggtgaatccc
      121 gctatcgctc tctgtccgca ctcggactgg gcctgccggc tgcgctgccg tgactgggct
      181 acttaacggg tgaatccacg cgcgatggcg tccaccaaac cacgagccgc acgccgactc
      241 ggatccaacg tcggatcgta gatggtgacc tccaaaccca cggcccgatc gcaggccaga
      301 gccgtgcgca ggatggagac gatttcatcc caggaaaacc catcgggcag gtgatacttc
      361 accgcgggca tgatcgcatc gtcgagcacg tcggcgtcga aatgaatcca gaaacctgcg
      421 agctccgcgc gcgtgagatg ggcgagcgct cgaccgagcg cgtgttccac gccgaggcgc
      481 cggacctcag ccaggtccat cgcgtggata ccgggcgcga gcggctggct gccgaagtgg
      541 cgctgctcgt ccagatcgcg aaagccgaaa acgaccacgt cctcgtcgtc gatgagacgc
      601 tcgtggtgtt cgaagcgggt gagtccgtga gggccgcggc ctgtggccag cgccagctcg
      661 ctggaagcgc cctccccatt cacgttcgcc tcgggctgat agaaatcggc gtgagcatcg
      721 atgaagagca ggccgaaacg gccgcgccgt cgcagtgcga ggaggttgcc cagcaggatc
      781 gtgcaatcgc cgcccagcac gagaggaaat tcgccgcggg ccagaatggg ctcgactgca
      841 tcggcgagcg tgaccgtgta gccggcgagc gtgtcggagt tgagcatacc gtgctccgga
      901 tggcgcaccg gattctgcgg cggcgcgtcg acgcgggcga tcaccggtgc attcagtcgc
      961 gcagccaagc cagcttccaa cagggcgtcg ccgagttgat tcacgccggt gccgtccaat
     1021 ccgaggactg acggagccag gagaatcacc ggttgcggtt gcattgtgct gttgctggac
     1081 aagcgcgacc gccctaaagc tccaccgccc aaccactgcc cggtcggaac ggaaatacgc
     1141 ccgatgccta gttcgaaag
//
LOCUS       DASPLT010000713         1029 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_68769, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000713 DASPLT010000000
VERSION     DASPLT010000713.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1029)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1029)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1029
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_68769"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..183
                     /locus_tag="VEA63_07480"
     CDS             <1..183
                     /locus_tag="VEA63_07480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HYD83877.1"
                     /translation="NKPVAAYQVSGEYAQLHAAAKLGWLDLARTRHESLLAIKRAGAD
                     MILTYFAKEMAAELKG"
     gene            complement(237..833)
                     /locus_tag="VEA63_07485"
     CDS             complement(237..833)
                     /locus_tag="VEA63_07485"
                     /inference="COORDINATES: protein motif:HMM:NF014606.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HYD83878.1"
                     /translation="MDFRRALPLALAALVATFLTACVTDDGAGKRVNPTIPEATSNAQ
                     LRSGDSLTIALQGVPDPSLNAVQIDDEGSISLPYIGTLAAAGANTGELAQRIRDTYIA
                     KKIYNTVDVSVSVTERFVYVGGEVVRPGRIIWTPDLTVTKAIQSAGGFTLYAKETRVN
                     LTREQNTYEIDTKLAQKNPAQDPRLVPGDAIQIPRSPF"
BASE COUNT          180 a          263 c          374 g          212 t
ORIGIN      
        1 aacaaaccgg tggcggcgta tcaggtgtcg ggcgaatacg cacaactgca cgcggcggcg
       61 aaactgggtt ggctggatct cgcgaggacg cggcatgagt cgttgctcgc gatcaagcgc
      121 gcgggcgcgg acatgatcct gacgtatttc gcgaaagaga tggcggcgga actgaaaggg
      181 tagggcgggc ttcagcccgc ttttcggcga cggcgggctg aagactgccc tacttgttag
      241 aacggcgagc gggggatttg gatggcgtcg cccgggacga ggcgggggtc ttgcgcggga
      301 tttttttgcg cgagtttcgt gtcgatctcg taggtgtttt gctcgcgcgt gaggttcacg
      361 cgagtttcct tcgcgtagag cgtgaaaccg cccgcggatt ggatcgcttt cgtcaccgta
      421 aggtcgggcg tccagatgat gcgaccggga cgaacgactt cgccgccgac atacacgaag
      481 cgttcggtca cgctgacgga aacgtcgacg gtgttgtaga ttttttttgc gatgtaggtg
      541 tcgcgaatgc gttgcgcgag ttcgccggtg tttgcgccgg cggcggcgag ggtgccgatg
      601 tagggaaggc tgatgctgcc ttcgtcgtcg atttgcacgg cgttcagcga gggatcgggc
      661 acgccttgaa gagcgatcgt caggctatcg ccgctgcgca attgagcgtt cgatgtcgcc
      721 tccggaatgg tggggttcac tcgcttgccg gcgccgtcgt cggtgacaca cgcggtgagg
      781 aaagtggcga ccagagcggc gagggcgagc ggcagcgcac ggcggaaatc catggcgcga
      841 gaattgggca acgcgcgacg ggaaagcaac gttgcgattg ggctggcaac ccgcgcgaac
      901 gcgcgccctg agaaggcgca agcgacacaa aaaaggccgc ctcgatgagg cggccctttg
      961 gctaaaggct agcgtttgcc tttggatttt ttgcgaggtt tctcgtcttc gtcgtcggat
     1021 ttttcgtcc
//
LOCUS       DASPLT010000714         1979 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_131933, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000714 DASPLT010000000
VERSION     DASPLT010000714.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1979)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1979)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1979
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_131933"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1868)
                     /locus_tag="VEA63_07490"
     CDS             complement(<1..1868)
                     /locus_tag="VEA63_07490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HYD83879.1"
                     /translation="MKKLWEVSTTEFGGGKESLMRRGNVYQIANGYMGYRGTLDEFGP
                     EESVGVTLAGIYDRVGSAWREPVNAPNAGFTRVSLDGAELSTLGRKVVRHRQTLQFAS
                     ATFERETVFMSRGKTLTLTSSRFLSAATPNLGVVRLSVRCDQPAVVTIRTGIDYNIWD
                     LNGPHLLELTAEKQDGILLVRGVTSEAGKQVVVAEAVECGFGEERHDIVGKRNLRVIR
                     FRAAPGTTYSLLKYFAVFTEHDRVEGTLVDAAAAVVRRGRSVGYAACRAAHEAEWVRK
                     WGRCDVVIAGDDEAQHALRYSILQLLMVAPVNGSANSIPARALSGQVYKGAVFWDTEM
                     FMFPFFLHTYPEKAVELLRYRIRTLDGARRKAKAEGPGYRGAFYAWESQETGDEACMY
                     FNIGDPFTGRELRTYFRDKQVHISGDVAIAMWHYFKVTGDDSLLLDGGAEVILECARF
                     FASYAYFQKDKRRYEILDVVGPDEYHERVNNNAFTSMVVKETFAIANATVEYLRRKHP
                     TAWGALVKKIRIERELPRFREAAELLYVPAPNPETGVIEQFDGYFQLRDATVEELKAR
                     MVHPNEYLGAGQGLAVPTKMIKQADVVMMLNLFKSRFSTDVKKANWNYYEPRTEHGS"
BASE COUNT          367 a          709 c          531 g          372 t
ORIGIN      
        1 gaaccgtgct ccgtgcgcgg ctcgtagtag ttccagttcg ccttcttcac gtcagtcgag
       61 aagcgcgact tgaacaggtt gagcatcatc acgacgtccg cctgtttgat catctttgtc
      121 ggcaccgcga gcccctgccc cgcgccgagg tattcgttcg gatgcaccat gcgcgccttc
      181 aactcctcga ccgtcgcgtc ccgcaattga aagtaaccgt cgaactgctc gatgacgccc
      241 gtctcgggat tcggcgccgg cacatacagc agttccgccg cttcgcgaaa gcgcggcagc
      301 tcgcgctcga tgcggatctt tttcaccagc gcgccccacg ccgttggatg cttccgccgc
      361 aaatactcca ccgtcgcatt cgcaatcgcg aaggtctcct tcaccaccat gctcgtgaag
      421 gcgttgttgt tgacccgctc gtgatattcg tcggggccga cgacatcgag aatctcgtag
      481 cgcctcttgt ccttctgaaa atacgcgtaa gacgcaaaga agcgcgcgca ctcgagaatg
      541 acctcggcgc cgccgtcgag cagcagcgaa tcgtcgccgg tcactttgaa gtaatgccac
      601 atcgcgatcg cgacgtcgcc gctgatgtgc acctgcttgt cgcgaaaata cgtgcgcagc
      661 tcgcgtcccg tgaagggatc gccgatgttg aaatacatgc aggcctcgtc gccggtctcc
      721 tgactctccc acgcgtaaaa cgcaccgcga taaccggggc cctccgcctt cgccttccgt
      781 cgcgcgccgt cgagcgtcct gattcggtag cgcaacagct cgaccgcttt ctccggatat
      841 gtatggagga aaaacggaaa catgaacatc tccgtatccc agaacaccgc gcccttatac
      901 acctgcccgg acagcgcgcg cgccggaatc gagttcgcgc tgccgttcac cggcgcgacc
      961 atcaacaact gcagaatgct atagcgcaac gcatgctgcg cctcgtcatc gcccgcgatc
     1021 acgacgtcgc atcgccccca ttttctcacc cactccgcct cgtgcgccgc gcgacacgcc
     1081 gcgtaaccca ccgaacgccc gcgccgcacc accgccgccg ccgcatcgac cagcgtcccc
     1141 tccacgcgat cgtgctcagt gaacacggcg aagtatttga gcaacgaata cgtcgtcccc
     1201 ggtgccgcgc gaaatcggat cacccgaaga tttcgcttac cgacgatgtc gtgccgttct
     1261 tcgccaaagc cgcactccac ggcttcggcg acgaccacct gctttcccgc ctcgctcgtc
     1321 acgccacgga cgagcaggat cccgtcctgc ttttccgccg tgagctcgag cagatgcgga
     1381 ccgttcaaat cccagatgtt gtagtcgatc ccggtgcgga tcgtcaccac ggccggttgg
     1441 tcgcagcgca cgctcaaccg cacgaccccg agattcggcg tggccgcgct caggaaacgc
     1501 gacgacgtga gcgtcagcgt tttcccgcgc gacatgaaaa ccgtttcacg ttcaaacgtc
     1561 gcgctcgcga actgcagcgt ctgccggtga cgcacgacct tgcgacccag tgtggaaagc
     1621 tccgcgccgt cgagcgacac ccgcgtgaac ccggcgttgg gcgcgttgac gggctcgcgc
     1681 cacgccgagc ccacgcgatc gtaaatcccc gcgagcgtga caccgaccga ttcctccggg
     1741 ccgaattcgt cgagcgtgcc gcgatagccc atgtagccgt tcgcgatttg atacacgttg
     1801 ccgcggcgca tcaggctttc cttgccgccg ccaaattcag tggtgctaac ctcccagagt
     1861 tttttcatgc gctaaattcg tctccgctcg tccttcaccc gcagcatcag cgccgcggcg
     1921 acaatcatcg caccaccgcc gagcacgagc gcgttgaccg cctggccgtc aaacacggt
//
LOCUS       DASPLT010000715         1107 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_9245, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000715 DASPLT010000000
VERSION     DASPLT010000715.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1107)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1107)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1107
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9245"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1107
                     /locus_tag="VEA63_07495"
     CDS             <1..>1107
                     /locus_tag="VEA63_07495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HYD83880.1"
                     /translation="EAFSAGAREGNDSLSPLLEKVAATFGLSDLDEASALFENVEHES
                     TWDTESLAHHRSYPEDALVAQERLQVEEDARAKFITQIATWKKDGYAIVFVVTKDGEE
                     QRIREILDENPKFKSIQPRFLRGALNEGFRVTFRADTPFDLASALCRAGSPDPAAPRA
                     RRGQETPPYTGLVVVTETEIFGRQRQRRAAPNARAVAQRAQIDQLLDFSELVEGDFVV
                     HLQHGIALYRGLTKLDTAQGVREVISLEFDDHVTLHVPLQESHLVSRYVGLSKARPQL
                     GRIGSGRWEKARAAAERATIDLAAELLRIHAAREAQPGFAFPPDNTWQKEFEASFPFT
                     ETRDQLRAIEESKADMERTRPMDRLICGDVGFGKT"
BASE COUNT          226 a          417 c          297 g          167 t
ORIGIN      
        1 cgaagccttc agcgctggcg cgcgtgaggg caacgacagc ctcagcccgc tgctcgaaaa
       61 agtcgccgcc accttcggcc tcagcgatct cgacgaagcc agcgcgcttt tcgagaacgt
      121 cgaacacgaa tccacctggg acaccgaaag tctcgcccat caccgcagct atcccgagga
      181 cgcgctcgtc gcccaggaac gcctgcaagt cgaagaagac gcccgcgcga aatttatcac
      241 ccaaattgcc acgtggaaaa aagacggcta cgcgatcgtt ttcgtcgtca ccaaagacgg
      301 cgaagaacaa cgcatccgcg aaattctaga cgagaatccc aagttcaaat ccatccagcc
      361 gcgcttcctc cgcggcgcct tgaacgaagg cttccgcgtc accttccgag ccgacacgcc
      421 attcgacctc gcgtcagcct tatgtagggc ggggtctccc gaccccgccg ctccgcgcgc
      481 ccggcggggt caggagaccc cgccctacac cggcctcgtc gtcgtcaccg aaaccgaaat
      541 cttcggccgc cagcgtcagc gccgcgccgc gcccaacgcc cgcgccgtcg cccaacgcgc
      601 gcagatcgat cagctcctcg atttctccga actggtcgaa ggcgacttcg tcgttcacct
      661 ccagcacggc atcgcgctct accgcggtct cacgaaactg gatacggccc agggcgtgcg
      721 cgaagtcatc tcgctcgagt tcgacgatca cgtcacgctg cacgttccgc tccaggaatc
      781 gcacctcgtc agccgctacg tcggcctcag taaagcgcgc ccacagctcg gccgcatcgg
      841 ctccggccgt tgggaaaaag cccgcgccgc cgccgaacgc gccacgatcg acctcgccgc
      901 cgaattgctt cgcatccacg ccgcccgcga agcgcagccc ggcttcgcct tcccgcccga
      961 caacacgtgg cagaaggaat tcgaagcgtc gttccctttc accgaaaccc gcgatcagct
     1021 ccgcgccatc gaggaatcga aggccgacat ggaacgcacg cgaccgatgg atcgcctgat
     1081 ctgtggcgac gtcggcttcg gcaaaac
//
LOCUS       DASPLT010000716         1898 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_68802, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000716 DASPLT010000000
VERSION     DASPLT010000716.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1898)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1898)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1898
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_68802"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1232)
                     /locus_tag="VEA63_07500"
     CDS             complement(<1..1232)
                     /locus_tag="VEA63_07500"
                     /inference="COORDINATES: protein motif:HMM:NF014594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase C-terminal
                     domain-containing protein"
                     /protein_id="HYD83881.1"
                     /translation="MPSSLDWKARAVSASDVISFVRSGHTLFVHGACATPTPLIEALC
                     ERRDLTDVRLYHLHTAGPAPFAARGREKEFRSVSLFTGAPLRQAIAEDRADFVPIFLS
                     DIPGLFLNGDVKLDVALVQVSPPDSHGLCSLGTSCDAAKAATETAKIIVAEINEQMPR
                     THGNNVLPFERIHAFTATDRPLIEHHPEPETAVEARIGELVANLVEDGSTLQMGIGGI
                     PDAVLARLHNKRDLGIHTEMFSDRVVDLVESGAVTNRLKAVGTGRLITSFINGTRRLF
                     DFVNDNPLVAFYPCDWTNDTSVIRKNPKVVAINSAIQIDLSGQVCADSIGHKVFSGIG
                     GQMDFIRGAALSRGGKPIIALPSTAAGGKISRLVVQLNAGAGVVTTRGHVHWVVTEYG
                     AVNLHGKTLRERAEALIS"
     gene            1623..>1898
                     /locus_tag="VEA63_07505"
     CDS             1623..>1898
                     /locus_tag="VEA63_07505"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HYD83882.1"
                     /translation="MPPLPTPPRIAILGAGITGLTAAWHLRRAGLTPVVFEKSAHFGG
                     AIGTVQSGGWLHELGPNSLLESSPAVAAFIDEIGLGERRIYASSAAKQ"
BASE COUNT          351 a          664 c          539 g          344 t
ORIGIN      
        1 atcgagatca gcgcctccgc gcgctcgcgc agcgtcttcc cgtgaaggtt caccgcgccg
       61 tattccgtca cgacccaatg cacgtgaccg cgcgtcgtca cgacccccgc acccgcgtta
      121 agctgcacca ccagcctcga aattttcccg cccgccgccg tcgagggcag cgcgatgatc
      181 ggcttgcccc cgcgcgacaa cgccgcgccg cgaatgaaat ccatttgtcc gccgattcca
      241 gaaaacacct tgtgcccgat cgagtccgcg cacacctgcc cgctcaaatc gatttgaatc
      301 gccgagttga tcgcgacgac tttcgggttc ttgcggatca ccgacgtgtc gttcgtccag
      361 tcgcacggat aaaacgcgac cagcggattg tcgttcacga agtcgaacag ccgccgcgtg
      421 ccgttgatga agctcgtgat caaccggccc gtgccgaccg ctttcaaccg gttcgtcacc
      481 gcgcccgact ccacgagatc gacgacccga tcggaaaaca tctccgtgtg aatcccgaga
      541 tcgcgcttgt tgtgcagccg cgcgagcacc gcgtccggaa tgccgccgat tcccatctgc
      601 aaggtcgaac catcctcgac caggttcgcc acgagttcgc cgatgcgcgc ttcgaccgcc
      661 gtctccggtt ccgggtgatg ctcgatcagc ggacgatccg tcgccgtgaa cgcatgaatg
      721 cgctcgaacg gcagcacgtt gttgccatgc gtgcgcggca tctgctcgtt gatttcggcg
      781 acaatgatct tcgccgtttc ggtggccgct ttcgccgcgt cacaagaagt gcccagcgag
      841 caaagcccat gcgagtccgg cggcgagacc tgcacgagcg cgacgtcgag tttgacgtcg
      901 ccgttgagaa acagtccggg aatgtccgac aaaaaaatcg gcacgaagtc cgcgcgatcc
      961 tcggcaatcg cctgccgcag cggcgcaccg gtgaaaagcg acaccgagcg aaattccttc
     1021 tcacgcccgc gcgcggcaaa cggcgccggc cccgccgtgt gaagatgata aagccgcacg
     1081 tcggtcaggt cgcggcgctc gcacagggct tcgatcagcg gcgtgggcgt cgcacaagca
     1141 ccatggacaa agagggtgtg gccgctccgc acaaacgaga tgacatcgct ggctgagacg
     1201 gcacgggctt tccaatcgag cgaagagggc atcggatgat ggtggcatct tacacagccc
     1261 cggtttcagc gaaagcgcgg accgtgcgca ggcgctgcca agcagtgcgc atttcttgtg
     1321 gacgcaaccc actcgcgttc gcgttcgcat tcacccttcg caagcgatgc gcagcctctc
     1381 gcaaaccgcg cgatgcagtc cgcccgcttc gccgtaagct ctgcgctcaa atactggatg
     1441 caaacccgag ccgtcatcgt cgtcagcatt cgtccgcatc gaagcccgcc aaacaccttg
     1501 tgcctcctgc ttcctcggtg tcggcgctcc gcgctctctg ggtaacaagg ctcgattgat
     1561 tcggtgcaat tcatccgggc taaagatcac ggctccctct tgccatgcct cctcctcttg
     1621 ccatgcctcc tcttcccacg cctccgcgaa tcgcgatcct cggtgccggc atcaccggat
     1681 tgacggccgc ctggcacctt cgtcgcgccg gcctcacgcc cgttgtgttc gaaaaatcgg
     1741 cccacttcgg cggagcgatc ggcacggtgc aaagcggcgg ctggctgcac gagctcggac
     1801 ccaattccct cctcgaaagc tcgccggccg tcgctgcgtt catcgacgaa atcgggctcg
     1861 gcgagcggcg gatttacgcc tcctccgccg ccaaacag
//
LOCUS       DASPLT010000717         1466 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_68808, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000717 DASPLT010000000
VERSION     DASPLT010000717.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1466)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1466)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1466
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_68808"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(387..>1466)
                     /locus_tag="VEA63_07510"
     CDS             complement(387..>1466)
                     /locus_tag="VEA63_07510"
                     /inference="COORDINATES: protein motif:HMM:NF028171.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase C-terminal
                     domain-containing protein"
                     /protein_id="HYD83883.1"
                     /translation="VNLLERPGTKAAAIEDVYWGTDIGGEMEIERELRGWSELVTFEK
                     RADMVTQAARLMAEGKVFGWVQGRSEFGPRALGNRSILADPRPAENKDIINRMVKKRE
                     AYRPFAPSVLAERVDEFFVLPGVQKDFGFMTFVVDVQPDKRVQLGAITHVDGTARVQT
                     VTRASNPRYWELIRAFGEITGVPILLNTSFNNHAEPIVDSVREAIACFLTTELHHLVI
                     GDFFVSKKNVVPADEALFGLAVTLPENLALRRERKRSADAADAGGAEGARGARWETTC
                     VATFTQHRGKSATLSDGMAAVLERANGVTSVSELAARAGVARTVEARAELAAELRELW
                     SNRVVVLRPVGESLDCLDRAVFLNR"
BASE COUNT          286 a          612 c          309 g          259 t
ORIGIN      
        1 agggcttttg tgcgcttcgg tgggaggggc tttacgcccc gacaaccgcc tccctctcca
       61 atcgccgtaa acccgccctc aatctcccct cgcatcccaa gaaaataatt gggacggacc
      121 tcaagcgagg tatcccggct gttttcattc accgcacgtc gcgctccact ccgctcactc
      181 catcgccccc gtaaacgcct ccttcagcaa cccgttctcc tgccccgctc cgtgacctct
      241 gtgtcccatg cctcggcatt tttcggcgtg atcgctctgc gctactggaa ccccggcgaa
      301 tcccgataaa acgtccgcgc tgcgccgatc aggcgaagca cgcactcctt cgttcgcacg
      361 attgacctca gaaaaatcag cgtagattag cggttcaaaa acacagcccg atcaaggcaa
      421 tcaaggctct cgccaaccgg ccgcagcacc accacgcggt tactccacaa ctcgcgcaac
      481 tccgccgcca gctccgcgcg tgcctccacc gttcgcgcca cacccgcacg cgccgccagc
      541 tcgctcacac tcgtcacacc attcgcccgc tcgagcaccg cagccatccc gtccgacaac
      601 gtcgcgcttt tcccacgatg ctgcgtaaac gtcgccacgc acgtcgtctc ccatcgcgca
      661 ccacgcgcgc cctccgcacc acctgcgtca gccgcatccg cgctccgctt ccgctcgcgc
      721 cgcaacgcca gattttccgg caacgtcacc gccaatccga acaacgcctc gtccgccggc
      781 accacgttct tcttcgacac aaagaaatcg ccgatcacca ggtggtgcag ctccgtcgtc
      841 agaaaacacg cgatcgcctc gcgcaccgaa tccacgatcg gctccgcatg attattgaac
      901 gacgtgttca ataaaatcgg cacgcccgtg atctcgccaa acgcgcggat caactcccaa
      961 taacgcggat tgctcgcgcg cgtcaccgtc tgcacccgcg ccgtcccatc cacgtgcgtg
     1021 atcgcaccga gctgcacccg cttgtccggc tgcacgtcca ccacaaacgt catgaaccca
     1081 aaatccttct gcacccccgg cagcacgaaa aactcgtcca cccgctccgc cagcaccgac
     1141 ggcgcaaacg gccggtaagc ctcccgcttc ttcaccatcc tattgatgat gtccttgttc
     1201 tccgccggac gcggatccgc cagaatgctc cggttcccca acgcccgcgg cccaaactcc
     1261 gaccgtcctt gcacccagcc aaacaccttc ccctccgcca tcaaccgcgc cgcctgcgtc
     1321 accatatccg cgcgcttctc gaacgtcacc aactcgctcc acccgcgcaa ctcccgctcg
     1381 atctccatct caccgccaat gtccgttccc caataaacat cctcgatcgc cgccgccttc
     1441 gtccccggcc gctccagcaa attcac
//
LOCUS       DASPLT010000718         1729 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_131962, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000718 DASPLT010000000
VERSION     DASPLT010000718.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1729)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1729)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1729
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_131962"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..467)
                     /locus_tag="VEA63_07515"
     CDS             complement(<1..467)
                     /locus_tag="VEA63_07515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012377393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0042279 - nitrite reductase (cytochrome,
                     ammonia-forming) activity [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-forming cytochrome c nitrite reductase
                     subunit c552"
                     /protein_id="HYD83884.1"
                     /translation="MSTPHRPSRFRDRRGVALWVYIAVLVAVGIATFLVLLLYQNIVA
                     RKAEATQHVFRVVDVDDATTDPALWGKNYPRQYDSYRRTVDIVETKHGGSEAFQHLDR
                     DPAWRRIFAGYAFAIDYREERGHAYMLSDQRETERVHQFKQPGSCLQCHGSVIP"
     gene            complement(529..1005)
                     /gene="nrfH"
                     /locus_tag="VEA63_07520"
     CDS             complement(529..1005)
                     /gene="nrfH"
                     /locus_tag="VEA63_07520"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012377392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase small subunit"
                     /protein_id="HYD83885.1"
                     /translation="MKLPSTRTRILLLLALASTFGLFAGVGTYTFYYAEGLSYFSNDP
                     AACVNCHIMREQYDGWLKSPHHAVATCNDCHTPHDLIGKYLTKAENGYWHSKGFTLQD
                     FHEPIMIRPKNSTVLQRNCVSCHQQIVSGINTHAGNRREMLDCVHCHRDVGHGPLI"
     gene            complement(1104..>1729)
                     /locus_tag="VEA63_07525"
     CDS             complement(1104..>1729)
                     /locus_tag="VEA63_07525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012377391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HYD83886.1"
                     /translation="NYRKHAAESGNDAPARPVLFIKATSSVQNPDDPIELPVKLASTR
                     VDYECELAIVIGKRCKNVPRAEALDYVLGYTCANDVSARDWQRDLGGGQWCQAKSFDT
                     FCPLGPVLVTKDQIPNPNDLRIRTILNGETLQDWNTNDMVFDVPALIEFLSSSKTLLP
                     GTVILTGTPHGVGFARQPPVWLKSGDTVSIDIEHIGTLTNPVISEPL"
BASE COUNT          311 a          424 c          646 g          348 t
ORIGIN      
        1 ggaatgactg agccgtggca ctggaggcag gagccgggct gcttgaactg gtgcacgcgc
       61 tccgtctcgc gttgatcgct cagcatgtag gcgtgtccgc gttcctcgcg gtaatcgatg
      121 gcgaaggcgt agccggcgaa aatgcggcgc caggccgggt cgcgatcgag gtgttgaaag
      181 gcttcgctgc cgccgtgttt cgtttcgacg atgtcgacgg tgcgtcggta gctgtcgtat
      241 tggcgcgggt agtttttgcc ccagagcgcg gggtcggtgg tggcatcgtc gacgtcgacc
      301 acgcggaaga cgtgctgcgt ggcctcggct ttgcgcgcaa cgatgttttg gtaaagcagg
      361 aggacgagga acgtggcgat gccgaccgcg acgaggacgg cgatgtaaac ccagagggcg
      421 acgccgcgac gatcgcgaaa acgggagggg cggtgaggag tgctcatggg gcaaaacgta
      481 gcgaagtggc ggatacgagc ggtgaagcgg aggggcgggc ggggcgaatc agatgagcgg
      541 gccgtgaccg acgtcgcggt ggcagtgcac gcagtcgagc atttcgcggc gattgcccgc
      601 gtgggtgttg atgcccgaga cgatttgctg gtggcagctc acgcagttgc gttgaaggac
      661 ggtggagttt ttcggccgga tcatgatcgg ctcgtgaaaa tcctggaggg tgaaaccttt
      721 cgaatgccag tagccgttct cggctttggt caggtatttt ccgatgaggt cgtgtggggt
      781 gtggcagtcg ttgcaggtgg cgacggcgtg atgaggagac tttagccagc catcgtattg
      841 ctcgcgcatg atatggcagt tcacgcaggc ggccggatcg ttggaaaaat acgagaggcc
      901 ctcggcgtaa tagaacgtgt aggtgccgac accggcgaag agcccgaacg tgctggcgag
      961 ggcgagcagc aggagaatgc gagtgcgagt cgacggcagt ttcacggggt atgaaacgcg
     1021 acgtctggtg gagactacgt cgcggcggct aaccgtatgg ttaaagcaaa ccgccggcag
     1081 tcaaatcgga gcgagacgaa ccgtcagagc ggctcgctga tcaccggatt ggtgagcgtg
     1141 ccgatgtgtt cgatgtcgat gctcacggtg tctccagatt tcaaccacac gggcggttgg
     1201 cgtgcaaagc cgacgccgtg cggcgtgccg gtcaggatga cggtgccggg cagcagggtt
     1261 ttgctgctgc tcagaaattc gatgagcgca ggcacgtcga agaccatgtc gttggtgttc
     1321 caatcctgca gcgtttcgcc gttcagaatc gtgcgaatgc ggagatcgtt ggggttcggt
     1381 atttgatctt tggtgacgag caccggtccg agtgggcaaa aggtgtcgaa gctcttcgcc
     1441 tggcaccatt ggccgccgcc gagatcgcgc tgccagtcgc gcgcggaaac gtcgttggcg
     1501 catgtgtagc cgaggacgta atcgagggct tcggcgcgcg ggacgttttt gcaacgtttg
     1561 ccgatgacga tggcgagttc gcattcgtag tcgacgcgcg tgctcgcgag cttgaccggg
     1621 agttcgatcg gatcgtcggg gttttgcacc gagctcgtcg ctttgatgaa gagcacgggg
     1681 cgggcggggg cgtcgttgcc gctttcggcg gcgtgcttcc ggtagttga
//
LOCUS       DASPLT010000719         1052 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_233687, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000719 DASPLT010000000
VERSION     DASPLT010000719.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1052)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1052)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1052
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_233687"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1052
                     /locus_tag="VEA63_07530"
     CDS             <1..>1052
                     /locus_tag="VEA63_07530"
                     /inference="COORDINATES: protein motif:HMM:NF012551.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="endo-1,4-beta-xylanase"
                     /protein_id="HYD83887.1"
                     /translation="VAQTPEGPPLATGLNKFLGGIYSASQQANFTAYFNQVTPENAGK
                     WGSVEATRDVMNWTELDAAYALAKENGFPFRFHVLVWGSQQPGWIENLPAPAQLEEIE
                     EWFAAVAARYPAIDYLEVVNEPTHAPPSGAGRGNYIAALGGAGASGWDWILNAFRMAR
                     LYFPATTKLVLNEYSVTNEPARMQTYIGIVNLLKAEGLIDAVGVQGHSFSTRSYSGNL
                     PETSAQTKASLDLLAATGMPIMVTEMDIDGGTPDAPNDAGQLSEYQRIFPIFWEHPAV
                     VGITLWGYRPGLWRNAQAAYIVRSDATERPAMTWLKETVRGERVAVAMPVTPLVSGSG
                     VVTLAAAASGTPAPSV"
BASE COUNT          186 a          290 c          386 g          190 t
ORIGIN      
        1 ggtcgcgcaa accccggaag gtccgccgct ggcgacgggg ttgaacaagt tcctcggtgg
       61 gatttacagc gcttcgcagc aggcgaattt caccgcctat ttcaatcagg tcacgccgga
      121 gaacgcgggc aaatggggca gtgtcgaagc cacgcgcgac gtgatgaatt ggacggagct
      181 cgacgccgcc tacgcgctcg cgaaggagaa tggttttccg tttcgtttcc acgtgctggt
      241 ttggggcagc cagcagccgg gctggatcga gaatctgccg gccccggcgc agttggagga
      301 aatcgaggaa tggtttgcgg cggtggcggc gcggtatccg gcgatcgatt acctcgaggt
      361 ggtcaacgag ccgacgcatg ctccgccgag cggggcgggg cggggcaact acatcgcggc
      421 gttgggcggc gcgggcgcgt cgggctggga ctggatcttg aacgcgtttc ggatggcgcg
      481 cttgtatttc cccgcgacga ccaagctggt gctcaacgaa tacagcgtga ccaatgaacc
      541 ggcgcgcatg cagacttaca tcggcatcgt gaatctcctg aaagccgagg gcctgatcga
      601 tgcggtgggc gtgcaggggc attcgttttc gacgcgctct tactcgggga acttgccgga
      661 gacctctgcg cagacgaagg ccagcctcga tctgctcgcg gcgacgggga tgccgatcat
      721 ggtaactgaa atggacatcg atggcggcac gccggatgcg ccgaacgatg cgggccagct
      781 ttccgagtat cagcggattt tcccgatctt ttgggagcat ccggcggtgg ttggcatcac
      841 gctttggggc tatcgaccgg gactgtggag aaacgcgcag gcggcttaca ttgttcgaag
      901 cgatgccacg gagcggccgg cgatgacctg gctcaaggag acggtgcgtg gcgaacgcgt
      961 ggcggtggcg atgccggtga ccccgcttgt gagcgggagc ggcgtggtga cgttagccgc
     1021 cgcggcgagc ggcacgccgg cgccgagtgt ga
//
LOCUS       DASPLT010000720         2156 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_193808, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000720 DASPLT010000000
VERSION     DASPLT010000720.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 2156)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2156)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2156
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_193808"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..781)
                     /locus_tag="VEA63_07535"
     CDS             complement(<1..781)
                     /locus_tag="VEA63_07535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HYD83888.1"
                     /translation="MSKLRILVLTSSTGGGHDARAEAFAEWCFQLYRHQVEVRIEQML
                     ERSSIVNRTGVRLYNRIQRSAPWVHKLFYALIELLAVLNRKRVTFGESYYTEVLREYQ
                     PHLVFSVHDCLNRGYFPLARKILGAENVRCATYCGEFSGGWGYSRNWVEPSADLYISR
                     TPTAHDFAVKRGISPERARVRGLLMQPRAHTEVFDEAKRRTFLTKKLGLREDRFTVFL
                     ATGGNGANNHFDMLPALLRHADKVQAIVICGRNRQTYNDVLH"
     gene            complement(784..1674)
                     /locus_tag="VEA63_07540"
     CDS             complement(784..1674)
                     /locus_tag="VEA63_07540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007360786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HYD83889.1"
                     /translation="MSASPPSTCAASSVANRSGVLAARYPTAFVTGASAGLGRAFAEM
                     LMGEGVRVWGTARDAARLTTLASAYPALFTPVVLDLADSAATEAAFARSARAADGAFA
                     LVINNAGYGVFGEFDATDFLVWQRQLDTMVTATLRIAHDALRGMRARGRGCIVNVASL
                     ATEFPLPFMSGYNVAKAALSAFSESLIVETAGTGISVIDFRLGDFRTDFNQAMQTASP
                     AATALNPRAKRAWQALEAHLRAAPPPSVAAGDLRRALLRGRSGTVRSGTWFQARIAPL
                     FARVVPASWRRAIAARYFGL"
     gene            complement(1671..>2156)
                     /locus_tag="VEA63_07545"
     CDS             complement(1671..>2156)
                     /locus_tag="VEA63_07545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HYD83890.1"
                     /translation="VTPYCEVLGWGQASDGYNVAISHPEGNGSAAAMTRALRASNVEP
                     AQVDYVNAHATSTIIGDVSEARALRAVFKEHAPRVAVSSTKSLTGHGLSLAGAMESGF
                     CAWAMREGFMPGSAHISRLDPECEGLRILRETENTAPNLVLKNSSGFGGANVALLFKR
                     T"
BASE COUNT          402 a          776 c          642 g          336 t
ORIGIN      
        1 agtgcagcac gtcgttgtag gtttgccggt tccgtccgca aatcacgatc gcctgcactt
       61 tgtccgcatg tcgcaacagc gcgggcaaca tgtcgaagtg gttgttcgcg ccattgccgc
      121 ccgtcgccag gaaaacggtg aagcggtcct cgcgcagccc gagcttcttc gtgagaaaag
      181 ttcgccgctt cgcttcgtcg aacacttccg tatgcgcccg cggctgcatc agcaaaccgc
      241 gcacccgcgc gcgctccggc gaaatgccgc gcttcaccgc gaaatcgtgc gccgtcggcg
      301 tgcgcgagat ataaagatcg gcgctcggct ccacccagtt gcgcgagtaa ccccagccgc
      361 cggaaaactc gccgcaatac gtcgcgcaac gcacgttctc cgcgccgagg atttttcgcg
      421 ccaacggaaa atacccgcga ttcaaacagt cgtggacgct gaacacgaga tgcggctgat
      481 actcgcgcag cacttcggtg taatagcttt cgccaaacgt cacgcgcttc cgattgagca
      541 ccgcgagcag ttcgatcagc gcgtagaaaa gtttatgcac ccacggcgcg ctgcgctgga
      601 tgcgattgta gagccgcacg cccgtgcggt tgacgatcga cgagcgctcg agcatctgct
      661 cgatccgcac ttcaacttga tgccggtaaa gttgaaaaca ccactcggca aacgcctcgg
      721 cgcgggcgtc gtgcccgccg ccggtgctcg aggtcagcac cagtatgcgc agcttcgaca
      781 cagtcagagc ccgaaatagc gggccgcgat cgcgcgacgc cacgaagccg gcaccacgcg
      841 cgcgaagagc ggcgcgatgc gagcttgaaa ccacgtgccg gagcgcaccg tgccgctccg
      901 cccgcgcaac aacgcacggc gcaaatcacc cgccgccacg ctcggtggag gcgcggcgcg
      961 caaatgagct tcgagcgcct gccacgcgcg cttggcacgt gggttcagcg cagtcgctgc
     1021 gggagacgcg gtttgcatgg cttggttgaa gtcggtgcgg aagtcgccaa gacggaaatc
     1081 gatcacgctg atccccgtgc ctgccgtttc aacgattaaa ctttcgctga acgcgctgag
     1141 cgccgccttc gcgacattgt agccgctcat gaacggcagc ggaaactcgg tcgcgagcga
     1201 ggccacgttc acaatgcatc cgcgaccacg cgcgcgcatg ccccgcagcg cgtcatgcgc
     1261 gatccgcagc gtcgccgtca ccatcgtgtc gagctggcgt tgccacacca aaaaatccgt
     1321 cgcgtcgaat tcgccgaaca cgccgtaacc cgcattgttg atcacgagcg caaacgctcc
     1381 gtccgccgca cgcgccgacc gcgcaaacgc cgcctccgtc gccgcggaat cggcgagatc
     1441 gagcaccacc ggcgtgaaca gcgccggata cgccgacgca agcgtcgtga gccgcgccgc
     1501 atcgcgcgcc gtgccccaca ctcgcacgcc ttcccccatc aacatctcgg caaacgcccg
     1561 ccctagcccg gcgctcgcgc ccgtcacgaa ggcggtcgga taacgcgcgg cgagcacgcc
     1621 ggaccggttg gcgacggacg acgcagcgca cgtcgatggc ggcgacgcgc tcacgtgcgt
     1681 ttgaacagca gcgcgacgtt cgcgccgccg aaaccgctgc tgttcttcaa cacaagattc
     1741 ggggcggtgt tctccgtctc gcgcaaaatc cgcagccctt cgcactcggg atccagccgc
     1801 gaaatatgcg ccgagcccgg cataaagcct tcgcgcatcg cccacgcgca aaacccgctc
     1861 tccatcgcgc cggcgagcga cagcccgtga ccggtcagcg atttcgtgct gctcaccgcg
     1921 acgcgcggcg cgtgctcctt gaacaccgct cgcaacgccc gcgcctccga cacatcgccg
     1981 atgatcgtcg aggtcgcgtg cgcgttcacg tagtcgacct gcgccggctc gacgttgctc
     2041 gcgcgcagcg cccgcgtcat cgccgccgcg gagccgttgc cctccgggtg cgagatcgcg
     2101 acattgtagc cgtccgacgc ctgcccccag cccagcactt cgcaatacgg cgtcac
//
LOCUS       DASPLT010000721         1032 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_193830, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000721 DASPLT010000000
VERSION     DASPLT010000721.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1032)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1032)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1032
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_193830"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..355
                     /locus_tag="VEA63_07550"
     CDS             <1..355
                     /locus_tag="VEA63_07550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HYD83891.1"
                     /translation="LNEATEYINPTKALEYMAAGRPVVSTALDEVKSNFSSVARIAKS
                     HTEFISLCQREVASPSQSRIARGLRLAAENTWEANVAKMEAHIAETLAARDAEAAAEA
                     SSPIVPLVGNQSAYV"
     gene            348..>1032
                     /locus_tag="VEA63_07555"
     CDS             348..>1032
                     /locus_tag="VEA63_07555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008767 - UDP-galactopyranose mutase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
                     /protein_id="HYD83892.1"
                     /translation="MFDYLIVGAGFAGSVLAERLARGQNKKVLIIDRRSHIGGNAYDC
                     HNEHGILIHQYGPHIFHTNSAEIFDYLSRFTEWRQYQHRVKAMVDGRLLPIPINLDTV
                     NELYGLQLTPEGMKTYLASVAEKKEKILTSEDVVVNGVGRDLYEKFFRNYTRKQWGLD
                     PSELDAQVTARVPVRFNRDDRYFTDSYQAMPARGYTHMFGNMLDHPNIKVMLNADYRE
                     VRDEVSFDQL"
BASE COUNT          227 a          358 c          259 g          188 t
ORIGIN      
        1 gctcaacgaa gcgaccgaat acatcaaccc gacaaaagcc ctcgaataca tggcggccgg
       61 ccgtcccgtt gtctccaccg ctctcgacga agtgaagtcc aacttcagca gcgtcgctcg
      121 tatcgcaaaa tcgcatacgg agttcatctc cctgtgtcag cgcgaagtcg cgtcgccttc
      181 ccaatctcgc atcgcccgcg gcctccggct cgccgcggaa aacacgtggg aagccaacgt
      241 cgctaaaatg gaagctcaca tcgccgaaac cctcgccgcc cgcgacgccg aagcggccgc
      301 cgaggcttcc tcccccatcg ttcctctcgt cggcaaccaa tccgcctatg tttgattatc
      361 tcatcgtcgg tgccggtttc gccggcagcg tcctcgccga acggctcgct cgtggacaaa
      421 acaagaaggt gctcatcatc gaccgccgct cgcacatcgg cggcaacgcc tacgattgcc
      481 acaacgagca cggcatcttg atccaccaat acgggccgca tatcttccac accaactccg
      541 ccgagatctt cgactacctg tcgcgcttca ccgaatggcg ccagtatcag catcgcgtaa
      601 aagcaatggt cgacggccgc ctgctgccga tcccgatcaa tctcgatacg gtcaacgagc
      661 tctacggcct ccaactcacg ccggagggaa tgaaaacgta tctcgcctcg gtcgcggaga
      721 aaaaagaaaa aatcctcacc tccgaggacg tcgtcgtgaa cggcgtcggt cgcgatctct
      781 acgaaaagtt cttccgcaat tacacgcgca agcagtgggg cctcgatccg tccgagctcg
      841 acgcgcaggt caccgctcgc gtgccggtgc gtttcaatcg cgacgatcgt tacttcaccg
      901 attcctacca ggcgatgccg gcgcgcggtt acacccatat gttcggcaac atgctcgatc
      961 acccgaacat caaggtgatg ctcaacgccg actaccgcga ggtgcgagac gaggtttcgt
     1021 tcgaccagct ca
//
LOCUS       DASPLT010000722         3366 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_9345, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000722 DASPLT010000000
VERSION     DASPLT010000722.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 3366)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3366)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3366
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9345"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..495
                     /locus_tag="VEA63_07560"
     CDS             <1..495
                     /locus_tag="VEA63_07560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012377368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HYD83893.1"
                     /translation="ALLELFYSSGLRVSELAGLMLQQVDLEHGFLRVFGKGAKERVVP
                     VGGKAAEALATYLAAGRPHFVRGRTGSRLFLNKHGAALSRVMLWMIVKKYAKRAGITK
                     NVKPHGLRHSFATHLLTGGADLRAIQEMLGHASIATTQIYTAVEPQRLLDHHAKYHPR
                     NRQA"
     gene            652..2073
                     /locus_tag="VEA63_07565"
     CDS             652..2073
                     /locus_tag="VEA63_07565"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HYD83894.1"
                     /translation="MLAARAAQPEAAVDPVEGFWCGTIRSHNEVSIYGLEFKRNDAGE
                     LVAVQYVPESNINGYTMGTIRAEGNRYLGAYDLTRRGDLLEGSFAGGGLPLSLRRTDG
                     PPSPHAPPRAPVGPAVVWSYTATGALWARPAVVEGVAYLGDVAGRLHAVDVADGSLRW
                     TRETGGAIYGEVLASGDVLYVVNDAGLLLAFDRVSGVERWRFVLGGGDRLRRPPSREK
                     DTEFDYAAPTPVLVDGVIYVGAVDGGFHAISATTGESVWRFEAEGGIRATALVIGERV
                     FFGTLKNHLVALDRANGKEVWRVDTGGQITTTPTLAAGNIVVGNRGYKLMAVRPDDGA
                     RVWEYFQWFSWVDSSPTVVDGVIYIGSSDQRKARALDAVDGGTQWATAIGGWAWAQPA
                     VTDDAVYTCTVGMADAFNYAHPVEGSINALDRKTGELKWRIPVPMPSEAYLSGAAAGP
                     VVTGDRLLIAGLDGVLRAYPLSR"
     gene            complement(2093..2413)
                     /locus_tag="VEA63_07570"
     CDS             complement(2093..2413)
                     /locus_tag="VEA63_07570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD83895.1"
                     /translation="MKTCSYIALALLVLGSGCSTAFRRTPASPHVPAVVHTPPSEKQL
                     QHRISELKRAGHSTDAAERKARREFAKDAWSYDNSHAWANEKARAERSAAREKMEKEL
                     AKLP"
     gene            complement(2423..2764)
                     /locus_tag="VEA63_07575"
     CDS             complement(2423..2764)
                     /locus_tag="VEA63_07575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD83896.1"
                     /translation="MNRLTPAVLLSFVVAGCTTKAVKTTEVHRPTTSTIAYAAFVERR
                     TDELQRMGGPFADRNIAATKARELGRSLYGEPTPDVSTTWSWGSEARRKEAQADVTET
                     LDKWDRNESRH"
     gene            complement(2842..3051)
                     /locus_tag="VEA63_07580"
     CDS             complement(2842..3051)
                     /locus_tag="VEA63_07580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD83897.1"
                     /translation="MKTIGIFFLWCLLLVLCWPLALLALVLAPLVWLIALPFRLLGVC
                     VHATFAFIKALLFLPARLLGGRVPA"
     gene            complement(3151..>3366)
                     /locus_tag="VEA63_07585"
     CDS             complement(3151..>3366)
                     /locus_tag="VEA63_07585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD83898.1"
                     /translation="PGDLARKITAIHTDADEFSVKARELYWLTRGKMSDSTVWTLPEV
                     RSLKLPKSTMRNTTTLRKIAEQYPPLA"
BASE COUNT          593 a          886 c         1228 g          659 t
ORIGIN      
        1 gcgctgttgg agttgtttta ttcgagcggg ctgcgtgtat ccgaattggc aggactgatg
       61 ttgcagcagg tcgacctgga gcacgggttt ttacgcgtgt tcggcaaggg tgcgaaagag
      121 cgcgtcgtgc cggtgggcgg gaaagcggcc gaggcgctcg cgacgtatct tgcggcgggg
      181 cggccgcact tcgtgagagg acgcacaggc agccggctgt tcctgaacaa gcacggtgcg
      241 gcgctttcgc gagtgatgtt gtggatgatc gtgaaaaaat acgcgaagcg cgcgggcatc
      301 acgaaaaacg tgaagccgca cgggctgcgg cattcgtttg cgacgcactt gttgacgggt
      361 ggcgcggacc tgcgcgcgat ccaggagatg ctcgggcacg cgagcatcgc gacgacgcag
      421 atctacacgg cggtcgagcc gcagcggctg ctcgatcatc atgcgaagta tcacccgcgg
      481 aatcggcagg cgtagtttcg cccacgaaga aaggtgaagg aaagacgtgg acgccggtgt
      541 tgaaccgctg tgcggtgccg tccacgcgtc gcaaacttcc acgcttcatc actcatgaga
      601 ttattttttc gccgccgttg ctttgctgtc ttcgtcgcat tttcgcttgg tttgctggca
      661 gctcgcgcgg cgcagccgga agcagcggtg gatccggtcg agggtttttg gtgcggcacg
      721 atccgatccc acaacgaggt ttcgatctac ggattggaat tcaaacgcaa tgacgcgggc
      781 gaactcgtgg cggtgcaata cgtgccggag tccaatatta atggatacac gatgggaacg
      841 atccgcgcgg agggaaaccg ctaccttggc gcctatgatc tgacgcggcg cggcgacttg
      901 ctcgagggat cgttcgcggg cggtggattg ccgctgagtc tgaggcgaac cgatggaccg
      961 ccctcgccgc atgcgcctcc gcgtgctccc gtgggtcccg cggtggtgtg gagttatacg
     1021 gcgacaggtg cgttgtgggc gcggccggcg gttgtcgagg gcgtcgccta tttaggcgac
     1081 gtcgccgggc ggttgcacgc tgtcgacgtg gcggatggca gcctgcgatg gacgcgtgag
     1141 acgggcggcg cgatttacgg cgaagtgttg gcgagcggcg acgtcctcta cgttgtgaac
     1201 gacgcggggc tgctgctggc attcgatcgc gtgtcgggcg tggagcgctg gcgtttcgtg
     1261 ttgggcggag gggaccgcct gcggcgtccg ccgagtcgtg agaaagacac ggagttcgac
     1321 tacgcggcgc cgacgccggt gctcgttgat ggcgtgattt atgtcggcgc ggtcgatggc
     1381 gggtttcatg cgatctcggc tacgacggga gaatccgttt ggcggttcga ggcggagggc
     1441 gggatccggg cgacggcgct cgtgatcggc gaacgcgtgt tttttggaac gttgaaaaac
     1501 catctcgttg cgctcgaccg ggcgaacggg aaagaagtgt ggcgcgtcga tacgggcggt
     1561 cagatcacaa cgacgccgac cttggcggcg ggaaatatcg tcgtaggaaa tcgcggctat
     1621 aaactgatgg cggtgcgccc ggacgatggc gcgcgcgtgt gggaatattt tcaatggttc
     1681 tcctgggtcg actcgagtcc gaccgtggtc gatggcgtga tctacatcgg ctcgtctgat
     1741 caacgaaagg cgcgggcgct cgatgcggtc gacggaggaa cgcagtgggc gacggcaatc
     1801 ggcgggtggg cgtgggcgca acccgcggtg acggacgacg cggtttatac ctgcacggtg
     1861 ggtatggccg atgcgtttaa ctacgcgcat cccgtcgaag gttcgatcaa cgcgctcgat
     1921 cggaagacgg gggagctcaa gtggcggatt ccggtgccga tgccgtcgga ggcttatctg
     1981 tctggagcgg cggcggggcc ggtggtgacg ggagatcgct tgctcatcgc gggcctcgac
     2041 ggcgtgttgc gcgcttatcc gctcagccga tagtcgaatc gggagacgtg agttacggga
     2101 gcttcgcgag ctccttctcc attttctcgc gagcggcgct acgttcggcg cgggctttct
     2161 cgttcgccca tgcgtgcgaa ttatcgtagc tccaggcgtc tttcgcgaac tcgcggcgcg
     2221 ctttgcgctc ggccgcatcg gtcgagtgtc cggcgcgttt tagttccgag atccgatgtt
     2281 gaagctgttt ctcgctgggt ggcgtatgga cgaccgctgg gacgtgaggc gaggcggggg
     2341 tccgtcggaa agccgtgctg caaccgctgc cgagcacgag gagagcgaga gcgatatagg
     2401 agcacgtttt catgagataa ctttagtgac gactctcgtt gcggtcccat ttatcgaggg
     2461 tctcggtgac gtccgcttga gcttctttcc tgcgcgcctc gctgccccag ctccacgtgg
     2521 tggagacgtc gggcgtcggc tcgccgtaaa gtgagcgccc caattcgcgg gctttggttg
     2581 cagcgatgtt gcggtcggca aacgggccgc ccatccgctg gagttcgtcg gtgcggcgct
     2641 ccacgaaggc tgcgtaagca atggtgctcg tcgtcgggcg atgcacttcg gtcgtcttga
     2701 ccgccttggt tgtgcagccg gcaacgacga agctcagcaa cacggcgggg gttaaccggt
     2761 tcatgccgca aaaaaactca cggttgcgcg aaggtcaatt cgccgcggct gggcgaatgg
     2821 gcgcggtggg cgtggacttg cttacgcggg aacgcgaccg ccgaggagac gagcgggaag
     2881 aaatagcaga gctttgatga aggcgaacgt ggcgtgaacg cacacgccga gaagccgaaa
     2941 gggcagggcg atcagccaga ccagcggcgc gagcacgagc gcgagcagcg ccagcggcca
     3001 gcagaggacg agaaggagac accagagaaa gaaaataccg atggttttca tggtggcggg
     3061 aaggagagga gagagggaaa gagagagaca gagtcgttcg ctcagagaac ccgtggcggt
     3121 cggtcgagtt gcaggtattt ccgcgcgcgg ctacgcgagc ggaggatact gctctgcgat
     3181 tttacgaagg gtggttgtgt tgcgcatggt ggatttggga agcttcaagg agcgcacttc
     3241 cggcagcgtc cacaccgtcg aatcgctcat tttgccacgc gtcagccagt agagctcacg
     3301 cgccttcacg ctgaactcgt cggcgtcggt gtggatggcg gtgattttgc gggcgagatc
     3361 gccagg
//
LOCUS       DASPLT010000723         1353 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_132102, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000723 DASPLT010000000
VERSION     DASPLT010000723.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1353)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1353)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1353
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_132102"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..781)
                     /locus_tag="VEA63_07590"
     CDS             complement(<1..781)
                     /locus_tag="VEA63_07590"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009513561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HYD83899.1"
                     /translation="MSTSAASTFLVIGERTNITGSPKFAKALKAGDWDACLAIARQQV
                     ESGANLIDINVDEALIDGEATMVKFLNLIAAEPEISRVPVMLDSSKWSVLEAGLKCIQ
                     GKGVVNSISLKDGEAEFLRRAKLLRLYGAAAVVMAFDEQGQAATRDEKVRICTRAYQL
                     LTERVGFPAEDIIFDPNILTVATGIEEHNNYAVDFFEATRIIKQTLPGAKVSGGVPNV
                     SFSFRGNNPVREAIHTVFLYHAIRAGLDMAIVNAGMLGVYEE"
     gene            complement(885..>1353)
                     /locus_tag="VEA63_07595"
     CDS             complement(885..>1353)
                     /locus_tag="VEA63_07595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007360562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HYD83900.1"
                     /translation="ESIFDERGYRLPVMISVTITDASGRTLSGQTVGAFYNSIRHVKP
                     FSVGINCALGGRAMRPYVEELAQLAECYVTCYPNAGLPNAFGGYDETPADMATVLGEF
                     AASGFVNAVGGCCGSTPAHIAAIAAAVRGAPPRSLPPRAATMQLSGLEPLIVA"
BASE COUNT          238 a          341 c          517 g          257 t
ORIGIN      
        1 tctcctcgta gacgccgagc atgccggcgt tgacgatcgc catgtcgagg ccggcgcgga
       61 tcgcgtgata gaggaaaacg gtgtggatcg cttcgcggac ggggttgttg ccgcggaagg
      121 aaaacgaaac gttggggacg ccgccggaga ctttcgcgcc gggcagcgtt tgcttgatga
      181 tccgcgtggc ctcgaagaaa tcgacggcgt agttgttgtg ctcctcgatg ccggtcgcga
      241 cggtgaggat gttgggatcg aaaatgatgt cctcggcggg gaagccgacg cgttcggtga
      301 ggagttggta ggcgcgtgtg cagatgcgga ccttttcgtc gcgggtggcg gcctggcctt
      361 gttcgtcgaa ggccatgacg acggcggcgg caccgtaaag gcggaggagt ttggcgcggc
      421 ggaggaactc ggcttcgccg tctttgaggg agatcgaatt aacgacgcct ttgccctgga
      481 tgcatttgag gccggcttcg aggacggacc atttcgagga gtcgagcatc acgggcacgc
      541 gcgaaatctc gggttcggcg gcgatgaggt tgaggaattt caccatcgtc gcttcgccgt
      601 cgatgagcgc ttcgtcgaca ttgatgtcga tgagattggc gccgctctcg acctgttggc
      661 gggcgatggc gaggcaggcg tcccaatcgc cggctttgag tgctttggcg aatttgggcg
      721 agccggtgat gttggtgcgt tcgccgatga cgaggaaggt ggaggcggcg gaggtggaca
      781 ttgttcagag gattggatcg ttctcgttct cttaatcgtt ctcgttctcg acgctttttc
      841 cttccgatcg agaacgagaa agagaacgag gaacgagaac gagtttaggc gacgatgagg
      901 ggttcgaggc cgctgagctg catggtcgcg gcgcgaggcg ggagcgagcg cggaggggcg
      961 ccgcgcacgg cggcggcgat cgcggcgatg tgggcgggcg tcgaaccgca gcagccgccg
     1021 acggcgttga cgaagccgct ggcggcgaac tcgccgagaa cggtggccat gtcggcgggc
     1081 gtttcgtcgt agccgccgaa cgcgttgggc aggccggcgt tggggtaaca ggtgacgtag
     1141 cactcggcga gttgcgcgag ttcctcgacg tagggacgca tcgcccggcc gccgagggcg
     1201 cagttgatgc cgacggagaa cggcttcacg tgacgaatgg agttgtagaa cgcgccgacg
     1261 gtctggccgg aaagcgtgcg accggacgcg tcggtgatcg tgacggaaat catcacgggc
     1321 aagcgatagc cgcgctcgtc gaaaatggat tcg
//
LOCUS       DASPLT010000724         1210 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_233759, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000724 DASPLT010000000
VERSION     DASPLT010000724.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1210)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1210)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1210
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_233759"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..381
                     /locus_tag="VEA63_07600"
     CDS             <1..381
                     /locus_tag="VEA63_07600"
                     /inference="COORDINATES: protein motif:HMM:NF024308.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative collagen-binding domain-containing
                     protein"
                     /protein_id="HYD83901.1"
                     /translation="SRDQSWDYARIALRFFREQQIPFWEMQPADELVGNSIGAESYYC
                     LAKPGELYLVYLPWGDVSTIDLRETSGEYSVKWFNPRTGGPLVEGPLKVVHGGKRNRI
                     GLPPTEENEDWVIVIRRNAGGNGN"
     gene            634..>1210
                     /locus_tag="VEA63_07605"
     CDS             634..>1210
                     /locus_tag="VEA63_07605"
                     /inference="COORDINATES: protein motif:HMM:NF024120.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 28-related protein"
                     /protein_id="HYD83902.1"
                     /translation="MNSPHQVCHSVADFGAASDGVTLAHDAFNHAIAACHQGGGGTVL
                     VPAGVYLVGTIELLSNVTLHLEAGAVIKGSPHLADYRVLPYTSEFRNTTLLLADGATN
                     IAITGRGTIDGNGAAFGIFDEADLSRDFDAKHTRQGEAYYAINDLPDDGPVKHKPRPG
                     ILILLVRCRDVVVRDIKLVDAPNWCLHVACSV"
BASE COUNT          261 a          312 c          399 g          238 t
ORIGIN      
        1 agtcgcgatc agagctggga ttacgctcgg atcgcgctgc ggtttttccg cgaacagcag
       61 attccgtttt gggaaatgca gccggcggac gaattggtgg ggaactcgat cggagcggag
      121 tcgtattact gtctcgcgaa gccgggtgag ctttacctcg tgtatctacc atggggcgac
      181 gtctcgacga tcgatctgcg ggaaacgtcg ggagaatact cggtgaagtg gttcaacccg
      241 cgcacgggcg gaccgctggt ggagggaccg ctgaaagtcg tgcacggtgg gaaacgaaac
      301 cgaatcggtt taccgccgac cgaggaaaac gaggactggg tgattgtgat caggaggaac
      361 gccggcggaa acggaaactg aacgggaacg gagttaaccg ctaattatcg ctaatggccg
      421 ctaatccaga ccgggatcat cggagtgatg accacgaaac ccacgaacga cacgaaatcg
      481 gaaggacgga gcggtggtga ttctcggtta aggccgatgc agccggacca tcgttcgcgg
      541 ctcgccgatc aggcgaggca tggtcagcgg agtttctctt ttacttcgga aaaatagcgt
      601 agattagcgg ttaaaaactt cagctttcgt ttcatgaact cacctcatca ggtctgccat
      661 agcgtcgcgg atttcggcgc ggccagcgat ggcgtcacgc tcgcgcatga cgcgttcaat
      721 catgccatcg cggcgtgtca ccaaggcggg ggcggcacgg tgctcgtgcc agcgggcgtt
      781 tacctggttg gaacgatcga gttgctcagc aacgtgacgt tgcacctcga agcgggggcg
      841 gtgatcaaag gcagcccgca tctcgccgat tatcgcgtgc tgccctacac gagcgagttt
      901 cggaatacga cgctgctgct cgccgacggc gcaacgaaca tcgcgatcac cggacgcggc
      961 acgatcgacg gaaatggcgc ggcatttggg attttcgacg aagcggattt gtcacgcgat
     1021 ttcgatgcga agcacacgcg gcagggcgaa gcctattatg cgatcaacga tttgccggac
     1081 gatggtccgg tgaagcacaa gccgcggcca ggaattttaa ttctgctcgt gcgttgtcgc
     1141 gacgtggtgg tgcgtgacat caagctcgtg gatgcgccga actggtgtct gcacgtggcg
     1201 tgcagcgtgg
//
LOCUS       DASPLT010000725         1269 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_32570, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000725 DASPLT010000000
VERSION     DASPLT010000725.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1269)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1269)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1269
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_32570"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..738)
                     /locus_tag="VEA63_07610"
     CDS             complement(<1..738)
                     /locus_tag="VEA63_07610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD83903.1"
                     /translation="MTGLVALRVGAADAVEGDAYARAGREAGTWQIGTGAVELTLDGR
                     DGGLKVTGFLNKLTRSSQNYIARDGATPAFLPEEQSGHEVETVWSKTLEGTQSADPAA
                     DRLRVRVEAGDLVGFEVHHFGDATGTETAWVTEVAYDDGERFLSSADTVLEQGPIWFY
                     AVHRRGARYFEYMDKVESLSEKIEYNDAGHVIGKGASSGEGEKIRCTTTYVPYAMPKA
                     APFANGTKIQPSRVNGAVRLWRAPKAGT"
     gene            complement(945..>1269)
                     /locus_tag="VEA63_07615"
     CDS             complement(945..>1269)
                     /locus_tag="VEA63_07615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009507741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HYD83904.1"
                     /translation="FAPLGPRLVTTDEIVNPNNLRISTRLNSERVQDSNTSDMIFDVP
                     ALIEYLSGSTTLVPGTVILTGTPQGVGMAAKPQPRWLRPGDEVSIEIEKIGTLTNRVV
                     WEGSS"
BASE COUNT          223 a          454 c          357 g          235 t
ORIGIN      
        1 ggtgccggct ttcggcgcgc gccaaagccg caccgcgcca ttcactcgcg aaggctggat
       61 cttcgtgcca ttcgcaaatg gcgccgcctt cggcatggcg tagggcacgt aggtcgtcgt
      121 gcagcgaatc ttctcaccct cgccagatga tgcgcccttg ccgataacgt ggccggcgtc
      181 gttgtattca attttctccg acagagactc gaccttgtcc atgtattcga aatagcgcgc
      241 gccgcgtcga tgcacggcgt aaaaccaaat cggcccctgt tccaggacag tgtccgccga
      301 cgacaggaac cgttcgccgt cgtcgtaggc gacttccgtc acccaagccg tctccgtccc
      361 cgtcgcatcg ccgaaatgat ggacctcgaa cccgacgaga tcacccgcct cgacccgcac
      421 gcgcagtcgg tccgccgccg gatcggcgga ctgcgttcct tcgagcgttt tgctccagac
      481 ggtttccacc tcgtggccgc tttgctcctc gggcaggaac gccggcgttg cgccgtcacg
      541 cgcaatgtag ttttgggatg acctcgttag cttgttgagg aaccccgtca ccttcaaccc
      601 gccgtcacgt ccgtcgagcg tcagctccac ggctcccgtg ccgatctgcc acgtgccggc
      661 ttcacggccg gcgcgggcat aagcatcgcc ctccaccgca tccgcagcgc cgactcgcag
      721 cgcaaccagg ccggtcatta ctccaacggc taaacacggg cgaagcgaaa atagtccgga
      781 aagcataagt gaaagaatgc ccgaccgcgg ttcgacccgc tacgcgtata cgccaaaatc
      841 ccgacgcttt cggctcccgc ctcaggcggc gactgccgtc cgacacagcg ccgttcgccg
      901 cacgcacccg gccgaggttc gagcgcaaac gcccgtcgca ccggtcagga cgaaccttcc
      961 cacaccaccc gatttgtcag cgtgccgatc ttctcgatct cgatgctgac ttcgtcaccc
     1021 ggccgcagcc agcgcggctg cggcttcgcg gccatgccca cgccctgcgg cgtgcccgtg
     1081 aggatcaccg tgccgggcac cagcgtcgtg ctgccgctca gatattcgat cagcgccggc
     1141 acgtcgaaaa tcatgtccga tgtatttgaa tcctggacac gctcgctatt caaccgcgtc
     1201 gagattcgca ggttgttggg attcacgatc tcgtcggtcg tcaccagtcg cggcccgagc
     1261 ggcgcaaag
//
LOCUS       DASPLT010000726         1434 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_48420, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000726 DASPLT010000000
VERSION     DASPLT010000726.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1434)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1434)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1434
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_48420"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1257)
                     /locus_tag="VEA63_07620"
     CDS             complement(<1..1257)
                     /locus_tag="VEA63_07620"
                     /inference="COORDINATES: protein motif:HMM:NF028199.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heparinase II/III family protein"
                     /protein_id="HYD83905.1"
                     /translation="MLARAVVIDGAFACASISAPMLFLSRRQFVEHAVLTAGAALCPS
                     ALRASNSSSVSAELAAGLGDRATRARHRIATVARFLDEAYPQLRRETIAAADEALRGE
                     LLLPGNRALSFVGDPPDWFTPRYGDEEYLWSLNRMMHWKTLLRAHALTGEEHYAAKVV
                     DELDDWIARARAPHFRHDDGTPNPHAVSNAGPPPWRSLEIGIRMFDTWPVVVEHLAGT
                     AHLPPARLARLAESVTQHAEALSLLTPLLWPDADHNHYFMEMLGLLSIGVRYRELPGA
                     ASLTNQAIHELQRCVRRQFTTDGGHVEGCPSYHNLCVVFLARFLSLAAIAQRELPADI
                     HQIAATSIEQTLHSTRPTGTIVPWGDSTQPENFAEAAVWFYKGSNDASVLQHLAALTG
                     LEKLQSLCAPFLWEIDDPADLFARMAN"
BASE COUNT          261 a          403 c          526 g          244 t
ORIGIN      
        1 attcgccatg cgcgcgaaaa gatcggcggg gtcgtcgatc tcccagagga acggcgcgca
       61 cagcgattgg agcttttcga ggccggtgag cgcggcgagg tgttgaagca cgctcgcgtc
      121 gttcgaccct ttgtagaacc agaccgccgc ctcggcgaaa ttttccggct gagtcgaatc
      181 gccccagggc acgatggtgc cggtggggcg cgtgctgtgg agcgtttgtt cgatactcgt
      241 ggccgcgatc tggtgaatgt ccgccggtag ctcgcgttgg gcgatcgcgg ccagagagag
      301 aaagcgcgcg aggaaaacga cgcatagatt gtgatacgaa gggcagcctt cgacatggcc
      361 gccgtcggtg gtgaactggc ggcgcacaca acgttgcagt tcgtgaatcg cctggttcgt
      421 caacgaagcc gcgcccggca gttcgcgata gcgcacaccg atcgaaagga gtccaagcat
      481 ctccatgaag tagtgattgt gatcggcgtc tggccagagg agcggcgtga gaagcgacag
      541 tgcttcggca tgttgggtga cgctctccgc gagacgcgcc agacgagcgg gtgggagatg
      601 cgcggtgccg gcgaggtgtt cgacaacaac cggccaggta tcgaacatcc gaataccgat
      661 ctcgagcgag cgccacggcg ggggaccggc gttgctcacc gcgtgcggat tgggtgtgcc
      721 gtcgtcgtgg cgaaaatgcg gcgcgcgggc gcgcgcgatc cagtcgtcga gttcgtcgac
      781 gactttcgcg gcgtaatgtt cctcgccggt cagcgcgtgg gcgcggagca gggttttcca
      841 gtgcatcatc cggttgaggc tccagaggta ttcttcgtcg ccgtagcggg gcgtaaacca
      901 gtcgggcggg tcaccgacga aggaaagcgc gcgattgccg ggaagaagca gttcgccgcg
      961 caaggcctcg tccgcagcgg cgatggtctc gcggcggagc tgcggataag cttcgtcgag
     1021 aaagcgcgcg acggtcgcga tgcggtgtcg cgcgcgagtg gcgcgatcgc ccagcccggc
     1081 ggcgagttcg gcggacacgc tcgacgagtt gctcgcgcgc aaggcggaag gacagagcgc
     1141 tgcgccggcg gtaagcacgg cgtgttcgac gaactggcgg cgggagagga aaagcatcgg
     1201 ggcggagatc gaagcgcacg cgaacgctcc gtcgatcacc acggcgcgcg cgagcacgag
     1261 cggggtctag cgtaaaccgc gcggcgctgg ttgcgtcaca cgattgtcac agatgcccgt
     1321 cgagagagga tttacgttaa acccgagaaa cactggtctc gccgattggc gagtgattgg
     1381 gatgccgccg atccttctgc ctcttcggtg gcgcgcagcg tttgtttcgc gtcg
//
LOCUS       DASPLT010000727         1394 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_132156, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000727 DASPLT010000000
VERSION     DASPLT010000727.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1394)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1394)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1394
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_132156"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..295)
                     /locus_tag="VEA63_07625"
     CDS             complement(<1..295)
                     /locus_tag="VEA63_07625"
                     /inference="COORDINATES: protein motif:HMM:NF019262.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome domain-containing protein"
                     /protein_id="HYD83906.1"
                     /translation="MRCPRERAPAARIACPLLVLVFSGALFFQAGCRPRLERTAEALP
                     DDLKAAGVAAAAPKLSFNEHVQPILSENCYACHGADAGSRKGELRLDRAEYAFA"
     gene            359..>1394
                     /locus_tag="VEA63_07630"
     CDS             359..>1394
                     /locus_tag="VEA63_07630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HYD83907.1"
                     /translation="MSSTALQQDDQTRTIGLAATRHFGMLGGMPTEPSRFASRMEQLR
                     PSTIREILKVTAQPEIISFAGGLPAPELFPVDAVSRAAQQVLASDGPSALQYGPSEGY
                     MPLREWIAAEMNRRGVKASSNEVLVTSGSQQVLDLAGKLFLNRGDVVLTENPTYLAAI
                     QAFQTMEARFVPVPTDDNGLIPDTIPELIRQHRPKFLYTIPNFQNPTGVTLAADRRQK
                     LARIAADHSLTILEDDPYGQLRYRGADIPPVKHWDEAGRVLYASTFSKVIAPGLRTGW
                     VVAPPEIFSKLLMLKQASDLHTSSLDQRIAYSFLTTSDVGAHLDRIRKSYGERFAVMD
                     ETLRAAMPSDY"
BASE COUNT          275 a          447 c          424 g          248 t
ORIGIN      
        1 gtgcgaatgc gtattccgcg cgatcgaggc ggagttcacc tttgcgtgaa ccggcgtcgg
       61 caccgtgaca cgcgtagcag ttttccgaca ggatcggctg aacgtgttcg ttgaaggaga
      121 gtttcggcgc agccgctgcg acccctgccg ccttcaaatc gtccggcaaa gcctcagcgg
      181 tccgctcgag acgcggacgg cacccggctt gaaaaaacaa cgcgccggaa aacaccaaca
      241 cgagcaaagg gcaggcgatt cgagcggcgg gggcgcgctc gcgggggcaa cgcatgtcct
      301 atcggtaaac cggcgcgacg ctgccgcaat accttcttcg cggattccgt tcacgcgctt
      361 gagcagcaca gcgcttcaac aagacgacca gacccgcaca atcggattgg ccgcgacgcg
      421 gcatttcggc atgttgggcg ggatgccgac cgagccgtca cgtttcgcga gccgcatgga
      481 gcaattgcgc ccctcgacca ttcgggaaat cctgaaggtc accgcgcaac cggaaatcat
      541 ttcgttcgcc ggcgggttgc ccgcgccgga attgtttccg gtcgacgccg tgagccgtgc
      601 cgcgcagcag gtgctcgcga gcgacgggcc gtccgcgttg caatacggcc caagcgaagg
      661 ctacatgccg ctccgggagt ggatcgcggc ggagatgaac cgccgcggcg tgaaagcctc
      721 atcgaacgaa gtgctcgtga cgagcggctc gcaacaggtg ctcgaccttg ccggaaaact
      781 tttcctcaac cgcggcgacg tcgtgttgac ggaaaacccg acctacctcg cggcgatcca
      841 ggcgtttcaa acgatggagg cgcgatttgt tcccgtgccg acggacgaca acggcctcat
      901 tcctgataca atcccggagc tgatccggca gcaccggccg aagtttctct acacgattcc
      961 gaactttcag aatccgacgg gcgtcacgct cgccgccgac cgccggcaaa aactcgcacg
     1021 catcgccgcc gatcattcgc tcacgattct cgaggatgat ccgtatggac aacttcgata
     1081 ccgcggggcc gacatcccgc ccgtgaagca ttgggacgaa gccggccgcg tgctttatgc
     1141 gagcacgttt tccaaggtga ttgcgcccgg gttgcgcacc ggctgggtcg tggcgccgcc
     1201 ggaaattttt tcgaagctgc tgatgttgaa acaggcgtcg gacctgcaca cctcgagtct
     1261 cgatcagcga atcgcgtatt ccttcctcac gacgagcgac gtcggcgctc acctcgatcg
     1321 cattcgcaaa tcttacggcg agcgtttcgc ggtgatggac gaaacgctcc gcgccgcgat
     1381 gccatctgac tatc
//
LOCUS       DASPLT010000728         1187 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_274483, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000728 DASPLT010000000
VERSION     DASPLT010000728.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1187)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1187)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1187
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_274483"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            209..967
                     /locus_tag="VEA63_07635"
     CDS             209..967
                     /locus_tag="VEA63_07635"
                     /inference="COORDINATES: protein motif:HMM:NF016826.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-deoxy-glucuronate isomerase"
                     /protein_id="HYD83908.1"
                     /translation="MSSSAVDHGLEFDLLVLGEGERFGETSGEESAWILLHGAAEIES
                     AGTRTAVVRGNVFDDPPSAIHLSADTPLQIRAIAADTEWAVVRTANERTFAPRVFLPV
                     DITPEYRGAGLVQNACLRNVRLIFDRASRPESNLVVGEVVNYPGRWSSYPPHHHDQPE
                     IYHYRFTHPDGYGHAELGDDVRKVRHKDTVVIPPGRDHAQVAAPGYGMYYLWMIRHLP
                     DNPYTGFTFAEEHKWTLNPAQQGWRPSDLPASLR"
     gene            964..>1187
                     /locus_tag="VEA63_07640"
     CDS             964..>1187
                     /locus_tag="VEA63_07640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD83909.1"
                     /translation="MKPTITLSMGAALVKYLQQQYIRRDGVEHRLINGVWGIFGHGNV
                     TGFGQALEEHGGRALPYYQAKNEQAMVHSAV"
BASE COUNT          221 a          383 c          366 g          217 t
ORIGIN      
        1 ttgtaggtgg cgcgcttgcg cgccacgtgg ggcgacgagc gcaccaccta cattgcagac
       61 ccgatcggag ggcaggcgcg gaggcctgcc gctacgcgac gatgctcccg attgttgacg
      121 ttaaaccaac ggcgcacctg ccgccgccac caatttccgg caagagacga atgtgcccct
      181 tgttcaacat ctggccggcc ccgccgccat gtcgtcatct gccgtggatc acggcctgga
      241 gttcgacctg ctcgtcctcg gcgaaggaga acggtttggc gagacctctg gcgaggagag
      301 tgcgtggatt ttgctgcacg gcgcggccga aatcgaatcg gccggcacgc gcaccgcggt
      361 cgttcgcggc aacgtcttcg acgatccgcc gagcgcgatt catcttagcg cagatacacc
      421 tctgcagatt cgcgccatcg cggccgatac cgaatgggcg gttgtgcgaa cggcgaacga
      481 gcggacgttt gcgccgcgcg tgtttctgcc ggtagatatc acgcctgaat atcgcggcgc
      541 ggggctcgtt caaaacgcct gcctgcgcaa tgtgcggctg attttcgacc gcgcgtcgcg
      601 gccggagtcg aacctggtcg tgggcgaagt cgtcaactat cccggacgct ggtccagcta
      661 cccgccgcat caccacgacc agcccgaaat ctatcattac cgtttcacgc atcccgacgg
      721 ctacggccac gccgagctgg gcgacgacgt gcgaaaagtt cggcacaaag acacggttgt
      781 gattccgccc ggccgcgatc acgcgcaggt cgcggcgccc ggttacggca tgtattatct
      841 ctggatgatt cggcacctgc cggacaatcc ctacaccggt ttcacgtttg cggaagagca
      901 caagtggacg ctcaatcccg cgcagcaagg ctggcgtccg tccgatctcc ccgcatctct
      961 ccgatgaaac cgacaatcac gttgagcatg ggcgctgcgc tggtgaagta tcttcagcag
     1021 caatacatcc gccgcgatgg cgtcgagcac cgcttgatca acggcgtgtg gggcatcttc
     1081 ggccacggta acgtcaccgg cttcggccag gcgctcgaag agcatggcgg ccgcgcgttg
     1141 ccttattatc aggcaaaaaa cgagcaggcg atggtgcact ccgccgt
//
LOCUS       DASPLT010000729         1055 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_9444, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000729 DASPLT010000000
VERSION     DASPLT010000729.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1055)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1055)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1055
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9444"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..364
                     /locus_tag="VEA63_07645"
     CDS             <1..364
                     /locus_tag="VEA63_07645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD83910.1"
                     /translation="QKRLADTVYFRNLANNAGVLDLLVGAGEDQDAKEAFLAYAVLRA
                     SERPLVKAEIDNAAEALLQERFGLDVDFEINDALAKIERLGLVAREGEDYRAVAPAEA
                     LAKLDAAWDDLFSFSARR"
     gene            complement(842..>1055)
                     /locus_tag="VEA63_07650"
     CDS             complement(842..>1055)
                     /locus_tag="VEA63_07650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD83911.1"
                     /translation="LFNLRDDPTEQHDVSAQHPERVAELRALIEAHNAQMPPPLWPAL
                     LEGPVRIDVPLNAPWTEEQEYVYWSN"
BASE COUNT          174 a          309 c          355 g          217 t
ORIGIN      
        1 tcagaagcgg ctggccgata cggtgtattt ccgcaacttg gccaacaatg ccggcgtgct
       61 cgatctgctg gtcggcgccg gcgaagacca agacgcgaaa gaagcgttcc tggcttacgc
      121 ggtgctgcgc gcctccgagc gcccgctggt gaaagcggaa attgacaatg cggcggaggc
      181 tttgctgcag gagcgctttg gtctcgatgt cgatttcgag atcaatgatg cgctggcgaa
      241 gatcgaacgc ttgggcctcg tggcgcgcga aggcgaagac tatcgcgccg tcgcgccggc
      301 cgaagcgctg gcgaaactcg acgcggcgtg ggacgatttg ttcagtttct ccgcgcgccg
      361 ctagcatact cttccctccg cgttgcaggc atggcgaggc cgcgagcctt ttgggctcgc
      421 ttcgagcttt cagcgttggt ctccctcgca aagggagccg gggcgcgcga tccgcgctcg
      481 ggtggtcgta gggtgcgctg ttgtacctct catcgaggcc cgatcgacat cccgcgtccg
      541 ccggctttct caacgatgat tggatttctc cacctcatcg aagcggcgcc tgccgcgccg
      601 gccgccgccg cacattccaa agtttcggac gtcttcgaaa tctcccgtgt gcgacggggc
      661 aagtgtactt gaggttggaa gcaatcggat agatttgcgc acatttttgc aggttggagc
      721 gcgggccttc aggcccgcat ggcgctagtg cttgaaagtt ggccgctaag cgggcctgaa
      781 ggcccgcggt ccaacatgca cgcagtggcg cgaattgggg gatggtgtcg gcgtctatcc
      841 atcaattcga ccagtagacg tattcctgct cttcggtcca tggcgcgttg agcggaacgt
      901 cgatacgcac tgggccttcc agcaaagcgg gccagagcgg cggcggcatt tgcgcgttgt
      961 gcgcttcgat caaggcgcgc agctcggcga cgcgttcggg gtgctgcgcc gaaacgtcgt
     1021 gctgctcggt cggatcgtcg cgcaggttga agagc
//
LOCUS       DASPLT010000730         1855 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_110610, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000730 DASPLT010000000
VERSION     DASPLT010000730.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1855)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1855)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1855
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_110610"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..619)
                     /locus_tag="VEA63_07655"
     CDS             complement(<1..619)
                     /locus_tag="VEA63_07655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD83912.1"
                     /translation="MPFPSPHRIRTALVCAAVLAAAFALAHSAAHAGAATSAPPKTII
                     FLAGPKDHGIPGRHEYEKDLRVLAHGLETSPNLPKLATKVFVGRAPRDLAELENAAAI
                     VIESSSDRLAKEVHPLFPPEPDNPNHTYDADTIAHLKALDALIAKNRIGVVVFHYANW
                     VEHWTARGYYLKWLGGLWVQMASRNPVDDWTMSPKTGDHPIFRGIQ"
     gene            complement(834..1565)
                     /gene="radC"
                     /locus_tag="VEA63_07660"
     CDS             complement(834..1565)
                     /gene="radC"
                     /locus_tag="VEA63_07660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009511796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HYD83913.1"
                     /translation="MSSSSATEYPANNRLRETAVGDRPQERLERLGAGALSDSELLAM
                     LLRSGTRGHDVLTLATRLIAEAGSLAGLIAWKEADFRRLKGIGRVKALQLLTVMEIAR
                     RVVGQQSSETPVLNRADLVAAHLQPVAHGLVVEKFWVLCLNRKNRLLRRVEVSSGTAT
                     AALAHPREVFREAIRESATAVICAHNHPSGDPAPSAADVHLTRQLREAAKAVDIALLD
                     HVVLGRPTADPLGRGYYSFREAGVL"
     gene            1611..>1855
                     /locus_tag="VEA63_07665"
     CDS             1611..>1855
                     /locus_tag="VEA63_07665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009510710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose utilization protein ThuA"
                     /protein_id="HYD83914.1"
                     /translation="MNPLRVVVWGENVHEHTNEAVRALYPHGMHETIAAAVRGLLPAA
                     SVTTATLQEPEHGLTEQRLAETDVLTWWGHKAHGDVS"
BASE COUNT          310 a          473 c          753 g          319 t
ORIGIN      
        1 gttggatgcc gcggaagatc gggtggtctc ccgtttttgg agacatcgtc cagtcgtcga
       61 cgggattgcg cgaggccatt tgcacccaaa ggccaccaag ccatttcagg taatagccgc
      121 gggcggtcca gtgttcgacc cagttggcgt agtgaaacac gacgacgccg atgcggtttt
      181 tcgcgatgag cgcgtcgagg gctttgaggt gggcgatggt gtcggcgtcg taggtgtgat
      241 tgggattatc gggttcgggc gggaagagcg ggtggacttc tttcgcgaga cggtcggagc
      301 tgctttcgat cacgatggcg gcggcgtttt cgagttcggc gagatcgcgg ggcgcgcggc
      361 cgacgaagac cttggttgcg agtttgggga gattgggcga ggtctcgaga ccgtgcgcga
      421 ggacgcgcag atctttttcg tattcgtggc ggccggggat gccgtgatct ttggggccgg
      481 cgaggaagat gatggttttc gggggggcgg atgtggcggc accggcgtga gcggcggagt
      541 gagcgagcgc gaaggcggcg gcgagaacgg cggcgcacac gagggccgtg cggatgcgat
      601 gaggcgaggg gaacggcatg gaggggaggc ggcgtgggcg gaaatgcgtg cggaaggaac
      661 cgaggaatct aacaggggaa cgaatcgcac gggcaaaagt tgcgacggtt gcgcgcgagc
      721 cggcggcgcg agttgaagct gtaggttgcg cgcttgcgcg caacgtggcg cgcaagcgcg
      781 ccacctacct gagattcggc aggcaggcac ggaggcctgc cgccgctacg ggatcagagg
      841 acgccggctt cgcggaagct gtagtagccg cggccgaggg gatcggccgt ggggcgaccg
      901 aggacgacgt gatcgagcaa cgcgatgtcg accgctttgg cggcttcgcg gagctggcgc
      961 gtgagatgaa cgtccgcggc gctcggcgcc ggatcgccgc tcgggtggtt gtgcgcgcag
     1021 atgaccgcgg tggcggattc gcggatggct tcgcgaaaca cttcgcgcgg gtgggcgagg
     1081 gcggccgtag cggtgccgga ggacacttcg acgcggcgga gcaggcggtt tttgcgattg
     1141 aggcagagca cccagaattt ttcgacgacc agaccgtggg cgacgggttg aaggtgcgcc
     1201 gcgacgagat cggcgcggtt aaggaccggg gtttcgctgg attgctggcc gacgacgcgg
     1261 cgggcgattt ccatcacggt gagaagttgc agcgctttga cgcggccgat gcctttgagg
     1321 cggcggaaat cggcctcttt ccaggcgata aggccggcga gggaaccggc ttcggcgatc
     1381 aggcgtgtag ccagcgtgag cacgtcgtgc ccgcgcgtgc cgctgcgaag gagcatggcg
     1441 aggagttcgc tatcgctgag cgcgccggcg ccgaggcgct cgagccgttc ctgcgggcgg
     1501 tcgccgacgg cggtttcgcg caggcgatta ttggcggggt attccgtcgc gctggagctc
     1561 gacacgaatt tcttttggac aggtttccgc gtaatgccaa cctttgactg atgaatccgt
     1621 tgcgagtcgt ggtgtgggga gaaaacgtgc acgagcacac gaacgaagcc gtgcgcgcgt
     1681 tgtatccgca tggcatgcac gagacgatcg cggcggccgt gcgcgggctg ctgccggcgg
     1741 cgagcgtgac gacggcgacg ctgcaggaac cggagcacgg gttaaccgag cagcggttgg
     1801 cggagacgga tgtgctgacg tggtgggggc acaaggcgca tggcgacgtg tcgga
//
LOCUS       DASPLT010000731         1502 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_132202, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000731 DASPLT010000000
VERSION     DASPLT010000731.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1502)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1502)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1502
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_132202"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..878)
                     /locus_tag="VEA63_07670"
     CDS             complement(<1..878)
                     /locus_tag="VEA63_07670"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HYD83915.1"
                     /translation="MSRSKPRTTKPVLGEDLDTLARSGAPRRFRAGEVIFSAGDAGDG
                     FYIVESGRVKISAPVGGNELRQLASIGAGDFFGEMAVLDDAPRSATATAEVATSTFFV
                     GRDQLLALLESRPPLALNLIREFSVRMRALNRKYLDEIIHAERLSIVGRFASTIVHDF
                     KNPLAVISLAAEASASTDLPEPTRLAAQKQIERQIHRMSNMLNELIDFTRPTKRAANF
                     EAVDFADYLRDLAQELQHDVAGREVTVVLANPPPSVEVQLQPTRYSRLFYNLVNNAVD
                     AMPDGGKVFLRSTVDGA"
     gene            complement(1012..>1502)
                     /locus_tag="VEA63_07675"
     CDS             complement(1012..>1502)
                     /locus_tag="VEA63_07675"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HYD83916.1"
                     /translation="VRVATHSDLPAIVEIYNSIIPGRMVTADLEPVTVESRLPWFDAH
                     DPARRPIWVAEENGTAIAWLSFDTYYPRAAYDGTAMIAIYVAEKKRGSGIGRMLLTRA
                     IEHAPSLGLRVLLGYIFAHNDPSLRLFTKFGFERWAYLPGVTRLDGVDRDVVIMGKRV
                     AP"
BASE COUNT          287 a          432 c          522 g          261 t
ORIGIN      
        1 gcgccgtcga cggtcgagcg gagaaacact tttccgccgt cgggcatcgc gtcgaccgcg
       61 ttgttgacga ggttgtagaa gagccgcgaa taacgcgtgg gctgaagctg aacttcaacc
      121 gacggcggcg gattggcgag gaccacggtg acctcgcggc cggcgacgtc gtgttgaagt
      181 tcttgcgcga gatcgcgaag gtagtcggcg aaatcgacgg cttcgaagtt ggccgctcgc
      241 ttcgtcgggc gggtaaaatc gatgagttcg ttcagcatgt tggacatgcg gtgaatctgc
      301 cgctcgatct gcttttgcgc ggccagtcga gtcggctccg gcaagtcggt gctcgcgctc
      361 gcctccgctg cgaggctgat gacggcgagc gggtttttga aatcgtggac gatcgtgctc
      421 gcgaaccggc cgacgatgct gaggcgctcg gcgtggatga tttcgtcgag atatttgcgg
      481 ttgagcgcgc gcatgcgcac gctgaattcg cgaatcagat tcaaggcgag cggcggacgg
      541 ctttccagca gcgcgagcag ttgatctcga ccgacgaaga acgtgctcgt cgcgacctcg
      601 gcggtcgcgg tcgcggagcg cggggcgtcg tcgagcacgg ccatctcgcc gaagaaatcg
      661 ccggcgccga tgctcgcgag ctggcgaagc tcgttgccgc cgacgggcgc ggagattttc
      721 acgcggccgg attcgacaat gtaaaagccg tcgccggcgt cgcccgcgga gaagattact
      781 tcgccggcgc gaaaccgccg cggcgctccc gagcgagcga gcgtgtccaa atcctcgccg
      841 aggaccggct tcgtggttcg tggtttcgac cgactcatgc aaccgccgaa cgtagaggcg
      901 cgaacgaagc agtaaaggcc ggaagccggt gagatcgctc ggagagcagg cagccacgga
      961 ggcctgccgc tacggacaac cgcggaaaca cggttatcgc ggccgagttg atcagggtgc
     1021 aacgcgtttg cccatgatca cgacgtcgcg gtcgacgccg tcgagacgcg tgacgccggg
     1081 aagatacgcc cagcgttcga aaccgaattt cgtgaagagg cgcagactcg ggtcgttgtg
     1141 ggcgaaaata taaccgagca acacacgcag accgagtgac ggcgcgtgct cgatcgcacg
     1201 tgtcagcagc atgcgaccga tcccgctacc acgctttttt tccgccacgt agatcgcgat
     1261 catcgcggtg ccatcgtagg cggcgcgggg ataatacgtg tcgaaactca accacgcgat
     1321 cgcagttccg ttttcttcgg cgacccagat cggacggcgg gcagggtcgt gcgcatcgaa
     1381 ccagggaagg cggctttcga cggtgaccgg ctcgagatcg gcggtgacca tgcggcccgg
     1441 gatgatcgag ttgtagatct cgacgatcgc agggagatcg gagtgcgtgg cgacgcgaac
     1501 ag
//
LOCUS       DASPLT010000732         1425 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_295781, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000732 DASPLT010000000
VERSION     DASPLT010000732.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1425)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1425)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1425
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_295781"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1300
                     /locus_tag="VEA63_07680"
     CDS             <1..1300
                     /locus_tag="VEA63_07680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HYD83917.1"
                     /translation="TGEPAAGIERYADFYLRGRDGWREGERDGKGREAAQFRTREVPA
                     SDGYSVVLSIDTAVQHIVEAELDYIAKTFQPKKATIVVTDPQTGFILALANYPSFDLN
                     TYNEVPRSQQHVMRNLAVTDMYEPGSTFKIVAAAGALNERLVTPGTLFDVSLDTVFYD
                     GEPRAMVRRMPRDDHPFHEPISVTDIIKHSSNRGAALLAMRLGDGKFYDYARAFGFGS
                     RTGFPVGGEHGPESFGMIAEPKDWDGLTITRMPMGHSVAATPLQMHMAMSVIASGGLL
                     LRPQVIKQIRDASGEVVYSYGPISKQRVVSETTAKAVGKMLMGVVGPDGPAKEIAIPH
                     YQVAGKTGTTQKIIDGKYSTRHHVASFVGFFPASRPQVAISVIVDDADAHAPNGVAYG
                     RTVAAPSFKRIGEQLIQYLDIKPVYSEAGGSNIVMGGVRQ"
BASE COUNT          266 a          403 c          485 g          271 t
ORIGIN      
        1 gaccggtgag ccggcggcgg ggatcgagcg ctatgcggat ttttatttgc gcggccgcga
       61 cggctggcgc gagggcgagc gcgatgggaa gggccgcgag gcggcgcagt ttcgcacgcg
      121 cgaagtgccg gcttcggacg gctattctgt cgtgttgtcg atcgacacgg cggtgcagca
      181 catcgtcgag gcggaactcg attacatcgc gaagacattt cagccgaaga aggcgacgat
      241 cgtcgtgacc gatccgcaga cgggcttcat tctcgcgctg gcgaattatc cgagcttcga
      301 cctcaacact tataacgagg tcccgcgatc gcagcagcat gtgatgcgca acctcgcggt
      361 gacggacatg tatgagcccg gctcgacgtt caagattgtc gccgcggcgg gcgcgttgaa
      421 cgagcggttg gtgacgccgg gcacgttgtt cgacgtgtcg ctcgacacgg ttttttacga
      481 cggcgagccg cgcgcgatgg tgcgccggat gccgcgcgat gatcatcctt ttcacgaacc
      541 gatttcggtc accgacatca tcaagcattc gagcaatcgc ggggcggcgt tgctcgctat
      601 gcgattgggc gacgggaaat tttacgatta cgcgagggcg tttggctttg ggagccgcac
      661 gggatttccg gtgggcggcg agcacgggcc ggaatcgttc ggcatgatcg cggagccgaa
      721 ggattgggat gggctgacaa tcacgcgcat gccgatgggg cactcggtgg cggcgacgcc
      781 gttgcagatg cacatggcga tgagcgtgat cgcgagcggc gggttgttgc tccggccgca
      841 ggtcatcaag cagatccgcg acgcgagcgg cgaagtggtt tacagctacg gaccgatttc
      901 gaagcagcgt gtggtcagcg agaccacggc aaaggcggtg ggcaagatgt tgatgggcgt
      961 ggtcgggccg gatggcccgg cgaaggaaat cgcgattccg cattaccagg tggcgggcaa
     1021 gaccggcacc acgcagaaga tcatcgacgg gaaatattcg acgcggcacc acgtggcgtc
     1081 gttcgtgggt ttctttccgg cgagccggcc gcaagttgcg atttccgtca tcgtcgacga
     1141 tgctgacgcg cacgcgccga acggtgtcgc atatgggcgc accgtcgcgg cgccgtcgtt
     1201 caagcgcatc ggtgaacaac tcatccagta tctcgatatc aagcccgtct attccgaggc
     1261 gggcggcagc aacatcgtca tgggaggagt tcgccagtga tcggttatct ttcgcaaaac
     1321 tatccactga ttcacgcctt cgcgccgcgg ccggctgttc gccgctcccg ccaggagaat
     1381 ctcatccgca atcgcgtctt taaaatggcc ccgaaactct ccgac
//
LOCUS       DASPLT010000733         2414 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_233859, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000733 DASPLT010000000
VERSION     DASPLT010000733.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 2414)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2414)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2414
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_233859"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..707)
                     /gene="prmC"
                     /locus_tag="VEA63_07685"
     CDS             complement(<1..707)
                     /gene="prmC"
                     /locus_tag="VEA63_07685"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HYD83918.1"
                     /translation="MLTVLEIIKKTTDFFAAKGVESPRLNAELIVGHGLALKRMQLYV
                     QFERLLSEVELERIRPMVRRRAQREPLQYVLGETEFFGLTLKTDRRALIPRPETELLV
                     ETIVAKLTAPPARILDLGTGTGAIALALAKTYADAAVVAVDASDEALSLARENSAKTA
                     LEGRVTWMKSDWFSDVPATERFDLIVSNPPYLSAAETGETSPEVRVHEPVGALTGKGV
                     DGLDDLRAIVRAAPAHLS"
     gene            complement(709..1836)
                     /gene="prfA"
                     /locus_tag="VEA63_07690"
     CDS             complement(709..1836)
                     /gene="prfA"
                     /locus_tag="VEA63_07690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HYD83919.1"
                     /translation="MEELPDIAPFQRRLDELDAQMADPAFYANPRRAAEISREQQKIQ
                     QLVADHQEFVRLEREADEAVAMSKDPAADPDLRELAVAELPELQRRRGALKEAVLLAM
                     IPPDPTDSRNTVMEIRAGTGGDEASLFAADLFRMYSRYADNRGWKIQPMSSSTSERGG
                     YKEVIFLITGTDVYKQLKFESGVHRVQRVPVTEANGRIHTSTVTVAVLPEAEEVDVQI
                     DPQDLEITVTRASGPGGQGVNTTDSAVQIVHKPTGLIVTCADERSQIKNKARAMTVLR
                     SRLLQRREDEERAKYAATRRSQIGSGDRSERIRTYNFPQNRLTDHRIGLTLYNLPQVM
                     EGGIEEIVAALQKADYEEKLAALTGVSLPGAPVRAARGDDD"
     gene            1954..>2414
                     /locus_tag="VEA63_07695"
     CDS             1954..>2414
                     /locus_tag="VEA63_07695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007360434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HYD83920.1"
                     /translation="MATTLFTQNTIACIWDFDKTLIPDYMQSPLFRRYGIDEATFWEE
                     TNKLAEHYRLRGYHLAPEISYLNHLLTHVLAGPLAGLNNKILRECGQEIRFYPGLPQF
                     FDRAKSFVQERPEYAKHDITLEHYVVSTGIAPMIRGSAIAKHIDGIWACEFT"
BASE COUNT          443 a          815 c          683 g          473 t
ORIGIN      
        1 gaaaggtgag ccggcgccgc gcggacgatc gctcgcaagt cgtccagccc atccacacct
       61 ttgccggtca acgctccgac tggctcgtgc acgcgcactt cgggcgacgt ctcccccgtt
      121 tccgccgccg acaaataagg cgggttagac acaatcaaat cgaaacgctc cgtcgcgggc
      181 acatccgaaa accagtcgga cttcatccag gtcactcgac cttcgagcgc cgtcttcgcc
      241 gaattctccc gcgccaacga tagagcctca tcgcttgcgt cgactgcaac cactgccgca
      301 tccgcatacg tcttcgcgag cgccagcgcg atcgcgcccg tgcctgtccc caaatccagg
      361 atacgcgccg gcggcgccgt caacttcgcg acgattgtct cgacgagcaa ctcggtctcc
      421 ggccgcggaa tcaaggcccg ccggtccgtc ttgagcgtga gcccgaaaaa ttcggtttcc
      481 cccagcacat actgcaacgg ttcgcgctgg gcgcgccgcc gaaccatggg gcgaatccgt
      541 tcgagttcca cttcggataa cagccgctcg aattgcacgt agagctgcat ccgcttcagc
      601 gcgaggccgt gtccgacgat caactcggcg ttcagccgcg ggctctcgac gcctttcgcg
      661 gcgaagaaat ccgtcgtctt tttaatgatc tcgagaacgg tgagcatctc aatcgtcgtc
      721 cccgcgcgcc gcgcgcacgg gcgcaccggg caaagatacg cccgtcagcg cggcgagctt
      781 ctcctcgtag tccgctttct gcagcgccgc tacgatttcc tcgatcccgc cttccatcac
      841 ttgaggaaga ttgtagagcg tcaggccgat tcggtgatcg gtgagccggt tctgcggaaa
      901 gttatacgtg cggatccgtt cgcttcgatc accggagccg atctgactgc ggcgcgtcgc
      961 cgcatatttc gcccgctcct cgtcctcgcg ccgttgcagc aggcgcgaac gaaggacggt
     1021 catcgcgcgc gccttgttct tgatctgcga acgctcatcg gcgcaggtga caatcagccc
     1081 ggtcggcttg tgcacgattt gaaccgcgga gtcggtcgtg ttgaccccct gcccgcccgg
     1141 cccgctcgct cgcgtcaccg tgatctcgag atcctgcgga tcgatctgca cgtccacctc
     1201 ctcggcttcc ggcagcacgg cgacggtgac ggtcgacgtg tggatgcgac cattcgcctc
     1261 cgtcaccgga acgcgttgca cgcgatgcac gccgctctcg aacttgagct gtttatagac
     1321 gtcggtgccg gtgatgagaa aaataacttc cttgtatcca ccgcgctcgg acgtgctgct
     1381 gctcatcggt tgaatcttcc aaccgcgatt gtccgcatag cgcgaataca tccgaaacaa
     1441 gtccgccgcg aacaagctcg cctcatcgcc gccggtgccc gcgcggattt ccatgaccgt
     1501 gttacgtgaa tcggtcggat caggcggaat catggcgagc agcaccgctt ccttcaacgc
     1561 gccccgccgc cgctgcaact cgggaagttc ggccaccgcg agctctcgca gatcgggatc
     1621 cgccgcggga tccttgctca tcgcgacggc ttcgtcagcc tcgcgttcca ggcgcacgaa
     1681 ctcctgatga tcggcgacga gctgctgaat tttctgctgc tcgcgcgaaa tctccgccgc
     1741 gcggcgcggg ttcgcgtaga aggcaggatc ggccatttgc gcatcgagct cgtcgaggcg
     1801 gcgttgaaac ggggcaatgt caggcagttc ttccatcgag ttcgggagcg agagcgagtg
     1861 gtggcgattt gcgaattgcg cgccccgccg catacggctt ggctgcccgc aatcgtctcg
     1921 ccaggcgcgt ccacctagcg ttgccgatca gcaatggcca ccacgctttt cactcaaaac
     1981 acgatcgcct gcatttggga tttcgacaag accttaatcc ccgattacat gcagtcgccg
     2041 ctcttccggc gctacggcat cgatgaggcg acgttctggg aggaaaccaa caagctcgcc
     2101 gagcactacc ggcttcgtgg ctatcatctg gcaccagaaa tcagttacct taatcacctg
     2161 ctcacccatg tgctcgccgg tccgctcgcc ggactgaaca acaagatact gcgcgagtgc
     2221 ggtcaggaaa ttcgcttcta ccccggcctg ccgcagtttt tcgatcgggc gaaaagtttc
     2281 gttcaggaac gccccgaata cgcgaaacac gacatcacgc tggagcacta cgtcgtcagc
     2341 acgggcatcg cccctatgat ccgcggcagc gcgatcgcca agcacatcga cggaatttgg
     2401 gcctgtgagt tcac
//
LOCUS       DASPLT010000734         1020 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_48535, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000734 DASPLT010000000
VERSION     DASPLT010000734.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1020)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1020)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1020
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_48535"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..829
                     /locus_tag="VEA63_07700"
     CDS             <1..829
                     /locus_tag="VEA63_07700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD83921.1"
                     /translation="DTARLALAAEILRQQDNLRSIEAGLPTIRDRRETQRAAVNRKTA
                     TVPAPQALAAATRAPNPVEVLRDNPELQLLKLAARRAHLETVYGLLWHRLGLSTEQTE
                     RFRDLFVAREEQLSDLLYATSKHGGVTNDPAVARLRTDLEVRHRTALRELLGDSSFRV
                     FEDYERTAPFRDTASALIGAATVSGLPFTQEQAENLTRVFAQANDDYRNGRWANAAGI
                     DWDVVKAEARGFLSNAQIEFLLTNEPRGRGAGGRFLPYFHRVVNEAQQADAAASQSP"
BASE COUNT          194 a          376 c          272 g          178 t
ORIGIN      
        1 ggacaccgcc cgtctggccc tggctgcgga aattctccgc cagcaagaca accttcgatc
       61 catcgaagcg ggccttccaa ccattcgcga tcgccgcgag acacaacgag ccgccgttaa
      121 ccggaaaacg gcaaccgttc ccgcgcctca agcgcttgcc gccgcgacaa gagcacccaa
      181 ccccgtcgag gtgttgcgcg acaatcccga gttgcaactc cttaaactcg ccgcccgtcg
      241 tgcccatctc gaaaccgttt acggcctttt atggcatcga ctcggtctct cgaccgagca
      301 gacggagcgg tttcgcgacc tcttcgtcgc ccgggaagag caactcagcg atctgctcta
      361 tgccacgtcg aagcatggcg gcgtcaccaa cgaccctgcc gtcgcccggc tccgcacgga
      421 tctcgaggtc cgccatcgca ccgcgctgcg cgaactactc ggcgattcct cgtttcgcgt
      481 gttcgaagac tatgagcgca cggccccctt ccgcgatacc gccagtgcgc tgatcggtgc
      541 ggccaccgtg tccggccttc ccttcactca ggagcaggcg gaaaacctca ctcgcgtctt
      601 cgcgcaggcg aacgacgatt atcgcaacgg tcgctgggcc aatgctgccg gtatcgattg
      661 ggacgtcgtg aaagcggaag cgcgcggttt tctctccaac gcgcaaatcg aattcctcct
      721 caccaacgaa ccgcgtggcc gtggagcagg aggccgcttc ctcccctatt ttcaccgcgt
      781 ggtgaacgag gcgcaacagg ccgatgccgc cgcatcccaa tcgccctgac atcccctcgg
      841 tttctcgtcc agcgcgggat gaaatgccgc ctcaagccac gctcgcgcac aacgccgagt
      901 aatgctcgcg cgcgcgcacc gcgcactcct ccaccgccgt ctccggcaga tgcgccagct
      961 tcagctcggt caaactgccg atccagtgac ttgtctcacc cggaagatcc aagccgaaac
//
LOCUS       DASPLT010000735         1135 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_152206, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000735 DASPLT010000000
VERSION     DASPLT010000735.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1135)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1135)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1135
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_152206"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..946
                     /locus_tag="VEA63_07705"
     CDS             <1..946
                     /locus_tag="VEA63_07705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD83922.1"
                     /translation="AVAVGTAVFQTREARKARDSLAAIQVERQQWLTRIEDLETRLQM
                     ATRHSRAADADNEAMVKAVAQASASAQARANPPVTEETVQARFRDAQQLARSGNWEGA
                     LPELLWCYDEGMVRIRSFTGVRNSFLLGELGRMAKDYPPALAALRERRDRAEKRMLAD
                     EDDRRAAMDVAALNSALGEKDRNMVLFDQLPENDPRREGLLLRVFDDLIAERRYREAA
                     AGRPAAKMLSQFERLTAERPIPEGISNPQMAKDINNANRRHVITSTAKHIEVLAGAGD
                     VEGARTLATRLLAYDASAETRELVEKHAARAGQPELIR"
BASE COUNT          235 a          327 c          386 g          187 t
ORIGIN      
        1 tgcggtcgcc gtgggcaccg cggtctttca aacgcgggag gctaggaagg cccgggattc
       61 cctcgctgcg attcaggttg aacgacaaca gtggttgaca cgtatcgagg atctggagac
      121 acggttgcag atggccacac gccactcccg agcggccgat gccgacaacg aggcgatggt
      181 aaaagccgtg gcacaagcaa gtgccagcgc gcaggcacgg gcgaatccac ccgttacaga
      241 ggagacggtg caggcgcgtt tccgtgatgc gcagcaactc gcgcgcagtg gaaactggga
      301 aggggcattg ccggaactgc tctggtgtta cgacgagggc atggtgcgga tccgaagctt
      361 caccggtgtg cgcaacagct ttctcctggg cgaactcggc cgcatggcga aagattatcc
      421 gccggccttg gccgcgctgc gcgagcgccg cgatcgcgct gaaaagcgaa tgctggccga
      481 cgaagacgac cgacgcgcag ccatggacgt ggcggcgctc aatagcgcgc tcggcgagaa
      541 agatcgtaac atggtgctgt tcgaccaact gccggaaaac gacccccggc gtgaaggact
      601 gctcctgcgc gtgttcgacg atctgatcgc cgagcgccgg tatcgtgaag cggccgccgg
      661 gcgaccggcg gcaaagatgt tgtcgcaatt cgaacggctg acggccgagc ggccgatacc
      721 ggaggggata tcgaatccgc agatggcaaa ggatatcaac aacgcgaatc gccgtcatgt
      781 gatcacgtcg acggcaaagc acatcgaggt tttggccggc gcaggggatg tcgaaggagc
      841 gcggacattg gcgacgcgac tgctggcgta tgacgcctcg gcggagacgc gggaactcgt
      901 ggagaaacac gccgcgcgcg cgggccagcc cgagttgatc aggtgagatt gtagcctgcg
      961 cccgcgagtc gttcttctac gattccgcgg cgcgcgctct tcggcttcac tcgaggatct
     1021 taagagggtc atggccgttg caggcgttag gcttgaaggc gatgttggct gtccgcatct
     1081 cgaaatcgcc tgcaagcagg ctctcacctc cgatttaaaa acgcgctcac gcagc
//
LOCUS       DASPLT010000736         2061 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_69123, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000736 DASPLT010000000
VERSION     DASPLT010000736.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 2061)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2061)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2061
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_69123"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2061
                     /locus_tag="VEA63_07710"
     CDS             <1..>2061
                     /locus_tag="VEA63_07710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD83923.1"
                     /translation="TLLSPHVDSVALTHSESFAVSHHRDGARTLAIIAGADARGTSHG
                     VYALLEKLGCGFYLSHTTRPPPRNSPLTFEAWTLSDEPLFDDRIVLNWHNFLSSATTW
                     EFADWERYIDASTRMRFNDLMVHAYGNNPMFTFTFNGVTKPVGYFATTQQGRDWGTQH
                     VNDVRRMIGGELFADAVFGASIAKADPAHRVAQTTALMQRVFTHARARGLGVSFALDV
                     DTPAANPQEIIRTLPPSARFTSGDYELANPDTPEGYAYYRAQVDQLLALYPNLTRLVV
                     WFRSPDSNTPWRRVRRSELPAAWQREFDGSDEDVSAFALAKVVKAFDRALKETGRRDI
                     TLAAGSWAFPFLEASDRYIPPEIPIIALDWWVSFDTARAQRDLRQVRSGRKLIPIVWA
                     HHDDRTYIGRPYTPFVDFASKLRRINASGFGIIHWTTRPLDLYFKSTLVQTWKTTADQ
                     PLEQTCDDMAARLFGESARAAGGRYLFSWVTEAPMFGRETSAHFIDHPLPTPLLHQKL
                     GRERLALLATIDAATLTPEGATQLRFFQRYEEFMLSFFASHTAFERAADALKHNDLAR
                     ARIEIAQANPHAVIRDYVTAARTGEITRGEEAILVSLNLRWLPHILSLRQAVGLEPVR
                     YRLGEVLQEPLAQGAYPNTFYFDDEQRLWKVIDAASAPSVLRLTAISGDKLAPGRYVV
                     NGIE"
BASE COUNT          369 a          820 c          506 g          366 t
ORIGIN      
        1 ggaccttgct ttctccgcac gtcgactccg tcgcgcttac acactccgaa tcgttcgccg
       61 tttcccacca ccgcgacggc gcgcgcaccc tcgcgatcat tgccggtgcc gacgcgcgcg
      121 gcaccagcca cggggtctac gcattgctcg aaaaactcgg ctgcggcttc tatctttccc
      181 acaccacccg cccgccgccg cgcaacagcc cgctcacctt cgaggcatgg acgctttccg
      241 acgagccttt gttcgatgac cgcatcgtcc tcaactggca caactttctc agcagcgcga
      301 cgacctggga atttgccgac tgggagcgct acatcgatgc ctccacccgc atgcgtttca
      361 acgacctcat ggtccacgcc tacggcaaca acccgatgtt cacgttcacg ttcaacggcg
      421 tcaccaaacc cgtcggttac ttcgccacca cgcaacaagg tcgcgactgg ggcacgcaac
      481 acgtcaacga cgtccgccgc atgatcggcg gcgagctttt cgccgacgcg gtcttcggcg
      541 caagtatcgc gaaagccgac cccgcccacc gcgtcgcgca aaccacggcg ctcatgcaac
      601 gcgtgttcac ccacgcccgc gcacgcggcc tcggcgtctc tttcgccctc gacgtcgaca
      661 cgcccgccgc gaatccgcag gaaatcatcc gcacgctgcc gccctccgcc cgtttcacga
      721 gcggcgatta cgaactcgcg aaccccgaca cgcccgaggg ctacgcctac tatcgcgcgc
      781 aagtcgatca actgctcgcc ctctacccga atctcacccg gctcgtcgtc tggttccgta
      841 gtcccgattc gaatacaccc tggcggcggg tgcgacgttc cgaactcccc gccgcgtggc
      901 agcgcgagtt cgacggctcc gacgaagacg tctccgcctt cgccctcgcg aaagtggtca
      961 aagccttcga ccgcgcgttg aaggaaaccg gccgccgcga catcacgctt gccgccggct
     1021 cctgggcgtt tcctttcctg gaagcctccg atcgttacat cccgcccgag atcccgatca
     1081 tcgcgctcga ctggtgggtc tccttcgaca ccgcccgcgc gcaacgcgat ctccgccagg
     1141 ttcgcagcgg gcggaaactc atccccatcg tttgggcgca tcacgatgac cgcacttaca
     1201 tcggccgtcc ttacacgccc ttcgtcgact tcgcgtcgaa gctccgccgc atcaacgcct
     1261 ccggcttcgg catcattcat tggaccaccc gcccgctcga cctgtatttc aaaagcacgc
     1321 tcgtccaaac ctggaaaacc accgccgacc aaccgctcga gcaaacctgc gacgacatgg
     1381 ccgcacgcct tttcggcgag tccgcccgcg ccgccggcgg ccgctatctt ttctcctggg
     1441 tcaccgaagc tccgatgttc ggccgtgaaa cctccgcgca tttcatcgac cacccgctgc
     1501 ccacgcccct gcttcatcaa aaactcggcc gcgaacgcct tgccctgctc gccaccatcg
     1561 atgccgcgac gctcacgccc gaaggcgcca cgcagctccg gttctttcaa cgttacgagg
     1621 agttcatgct ttccttcttc gcgagccaca ccgctttcga gcgcgccgcc gacgccctga
     1681 aacacaacga cctcgcgcgc gcccgcatcg aaatcgccca ggccaatccc cacgccgtca
     1741 ttcgcgatta tgtcaccgcc gcccgcaccg gcgaaatcac aagaggcgag gaagcgatcc
     1801 tcgtttccct caacctccgc tggctgccgc acatactttc actgcgccag gccgtcggcc
     1861 tcgaacccgt ccgttaccgc ctcggcgaag tgctccagga gcctctcgcc caaggcgcgt
     1921 atccaaatac cttctacttc gacgacgaac aacgcctctg gaaagtaatc gacgccgcct
     1981 ccgcgccatc cgttcttcgc ctcaccgcca tctccggcga caaactcgcc cccggtcgct
     2041 acgtcgtcaa cggcatcgaa a
//
LOCUS       DASPLT010000737         2280 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_132308, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000737 DASPLT010000000
VERSION     DASPLT010000737.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 2280)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2280)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2280
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_132308"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            125..922
                     /locus_tag="VEA63_07715"
     CDS             125..922
                     /locus_tag="VEA63_07715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD83924.1"
                     /translation="MNRLQTGSYLPKGMVAGLAAFALQLFVDFHFKIPALAMAFGVVA
                     ALVVQEFWPVASEANARWRKARRFGCVAGALAMVAGVWLFARPVYLGEALRYGARQEI
                     DRIAVRRLPEGEWPPVLSRVSADLQRSLALAPRNGQAWAECAYANALWARAEPAKAGQ
                     HGAAALDAADRALACSTVVPEFWLRRGVALDVLGRRVEAGAAFVEALKLAPANALVWY
                     YHAFHLSLDRTQPGRAVAAARVCLRLDPQNQDAQALLQRLADRSRSR"
     gene            933..1892
                     /locus_tag="VEA63_07720"
     CDS             933..1892
                     /locus_tag="VEA63_07720"
                     /inference="COORDINATES: protein motif:HMM:NF016648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR family protein"
                     /protein_id="HYD83925.1"
                     /translation="MRLIKSFSWLLLCGALAFVATAAHAQPSTRVAGKIVAARVVGTV
                     TATDRASGVAQTLANGAELSQNHQITTGAGSSVVLVFSNGASLNLGAESTLAIEEFLQ
                     DPFTTPVSPADMTEEPTTSTTKLNLTRGELVGNVKHLKHEQGSTFEVQTPVGAAGIRG
                     TTFRIVFRPDASGRVFFTLSTADGTVLFEAPAAEGVSVETGNEVVVTVDVTVDAENAT
                     VTMNSTPEVGSVLPMSPATEALIAVQAQQIIADSATVILTTTTSEMTTPAGGLPPSNE
                     GDGGENEQTQEAQDDPAFTPSNDTTVPIPGPIKNTTPGDGKTI"
BASE COUNT          365 a          635 c          877 g          403 t
ORIGIN      
        1 tgtggggttc gcgctgttct tcggcgcgtg cgcggtggtg ggagcgggtt cgtggcgggt
       61 gacgcgaagg cgcgcagact gtttgggttc aacagcccca cctgcgcttt cccgcctccg
      121 tcgcgtgaat cgcctgcaaa caggctccta ccttccgaaa gggatggtcg cgggcctggc
      181 ggcgtttgcg ctgcagttgt tcgtggattt tcacttcaag attccggcgc tcgcgatggc
      241 gttcggtgtg gtcgcggcgc tggtggtgca ggaattctgg ccggtggcgt cggaggcaaa
      301 cgcgcggtgg cggaaggcgc ggcgtttcgg atgcgtggcg ggagcgctcg cgatggtagc
      361 gggcgtctgg ttgtttgcgc gaccggttta tctgggggag gcgctgcgct acggcgcgcg
      421 gcaagagatc gatcggattg cggtgcggcg actgccggaa ggcgagtggc cgccggtgct
      481 gagtcgggtg agcgcggatc tgcagcgttc gctcgcgctg gcgccgcgca acggacaggc
      541 gtgggcggaa tgcgcgtatg cgaacgcgct ttgggcgcgc gccgagccgg cgaaagcggg
      601 gcaacatggc gcggcggcgt tggacgcggc ggatcgcgcg ctggcgtgct cgacggtggt
      661 gccggagttt tggctgcggc gtggagtcgc gctcgatgtg ttggggcggc gggtcgaggc
      721 gggggcggcg tttgtcgaag cgctgaaact cgcgccggcg aacgcacttg tttggtatta
      781 ccacgcgttt catttgagcc tggatcgcac tcagccgggc cgggcggtgg cggcggcgag
      841 agtctgcttg cgccttgacc ctcaaaacca ggacgcgcaa gcattgctcc agcgtctggc
      901 cgaccgcagt cgctcccgtt aatccagctc tcatgcgact gatcaaatcg ttttcgtggc
      961 tgttgctctg cggggcactg gccttcgtcg caaccgcggc acacgcgcag ccatcgaccc
     1021 gggttgcggg caagatcgtg gccgcgcgcg tggtcggcac ggtgacggcg acggatcgcg
     1081 cgagcggcgt cgcgcaaacg ctcgcgaacg gcgccgagct gtcgcagaac caccagatca
     1141 cgacgggcgc gggttccagc gtggtgctgg tgttttcgaa tggcgcgtcg ttgaacctcg
     1201 gagcggaatc gacgctggcg atcgaggaat ttttgcagga tccgttcacg acgccggtgt
     1261 cgcccgcgga catgacggag gagccgacca cgtcgacgac gaagttgaat ctcacgcgcg
     1321 gtgaattggt cggcaacgtg aagcatttga aacatgagca gggctcgacc ttcgaggtgc
     1381 aaacgccggt cggcgcggcg gggattcgcg gcacgacgtt ccggattgtg ttccggccgg
     1441 atgcgagcgg gcgggtgttt ttcacgctga gcacggcgga cggcacggtg ttgttcgaag
     1501 cgccggcggc cgagggcgtg agcgtggaaa ccggcaacga ggtcgtggtg acggttgacg
     1561 tgaccgtcga cgccgagaac gccacggtga cgatgaactc gacgcctgaa gtgggctcgg
     1621 tcctgccgat gtcgccggcg acggaggcgt tgatcgcggt gcaggcgcag cagatcattg
     1681 cggactcggc gacggtgatc ctcaccacga cgacgagtga gatgacgacg ccggcgggcg
     1741 ggcttccgcc gtcgaacgaa ggcgacggcg gcgagaacga acagacgcaa gaggcgcagg
     1801 acgatccggc gttcacgccg tcgaacgaca cgaccgtgcc gattccgggg ccgatcaaga
     1861 acacgacgcc gggcgacggg aagacgatct gagcggtgtt gatggaggga agcggcggag
     1921 ctgctgacag cgctgcgtgg tcgaaggtgg aacccgacga gaactgtgtt cgtcgggttg
     1981 ctttgcgagc gagcgtcgtt caacccgatg aggacattgg gctccgccgc tgaacgcgac
     2041 aagggcaacg ggaacggctc gccggggacg gctcgcccta ccttgtgtcg tgaggtgagg
     2101 acgcggtttc acctttgtcc attgaagctt cgagcttcaa tggagcttgg aggttggagc
     2161 ttggtgcttc gccgccgatc ggcggcgcgc ggcgcgtcct caaatcatcg gaacgtgcgg
     2221 ctcgacgtcg gcatcgtagt cgatgccggg gaggccgaaa ccgaagagac ggaggaattc
//
LOCUS       DASPLT010000738         1121 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_110741, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000738 DASPLT010000000
VERSION     DASPLT010000738.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1121)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1121)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1121
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_110741"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..302)
                     /locus_tag="VEA63_07725"
     CDS             complement(<1..302)
                     /locus_tag="VEA63_07725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5060 domain-containing protein"
                     /protein_id="HYD83926.1"
                     /translation="MLMRFVRCIGCVVLANMLVAVSDAAGAAAAIGGELKQWHKITLS
                     FAGPQVSETDAKNPFLDYRLDVSFTHAASGRTLVVPGYFAADGNAANTGAKAGNVWR"
     gene            651..>1121
                     /locus_tag="VEA63_07730"
     CDS             651..>1121
                     /locus_tag="VEA63_07730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD83927.1"
                     /translation="MKFFSLALAGLLAIPFHDAVAASRPAVTVQIVPAKIVRRTFDPQ
                     RPPADMPKLVPPEVGQCVYEFGCEMETRTERPALPVGSPRARVTATSITARLNITLWT
                     PRGGPPAIVEHEEAHREICEIYYRPAAEIARRLGERMVGTTLPLTSRSQAAAEAA"
BASE COUNT          195 a          404 c          306 g          216 t
ORIGIN      
        1 cgccacacgt tgccagcctt cgcgccggtg ttggccgcat tgccgtcggc cgcgaaatag
       61 cctgggacca ccagcgtgcg gcccgacgcc gcgtgcgtga agctcacatc gagccggtag
      121 tcgagaaacg gattctttgc atccgtctcg ctcacctgcg ggcccgcaaa cgacagcgtg
      181 atcttgtgcc attgcttcaa ctcaccgccg atcgcggccg ccgctcccgc cgcgtcgctc
      241 accgccacca gcatgttcgc caacaccacg catccaatac accgcacaaa tcgcattaac
      301 atgtttcgtc tattttgaag gttactgaga acgccggccc acgtgcgccg ccgccgttgt
      361 cgctggcagg aatcgagcgc acagtgcggc ggacgtctgc tccgtcgaga aatttgatta
      421 cgccgggcag cgcgagaaat cctatgatca acataggaat tttccctaag ctcttctttg
      481 cgaattccgc tcggcgcgtg acgtgcgaca atcgccgaaa atccaccgcg ttatctaaat
      541 ccctttttta cagctccacc gctcacgatg cgccactccg cttttcgcgt tgatcgcgtc
      601 ccgcactcgc cctgatctcc gcgcacgtcg tccgtgagca cacctcgtcc atgaaatttt
      661 tctcgttggc cctggccggg ctgctcgcga ttccctttca cgacgccgtc gcggcttccc
      721 ggcccgcggt caccgtccaa atcgtgcccg caaaaatcgt tcgtcgcact ttcgatccgc
      781 agcgccctcc ggccgacatg ccgaaactcg tgccgcccga ggtcggtcaa tgcgtttacg
      841 agttcggttg cgaaatggag acgcgcacgg aacggcctgc gcttcccgtc ggctcgccac
      901 gcgcgcgtgt cactgccacg tcgatcacgg cgcgcttgaa catcacgctg tggacgccgc
      961 gcggcggtcc gcccgcgatc gtcgaacacg aggaggctca ccgcgaaatc tgcgaaatct
     1021 attaccgccc cgccgccgag atcgcgcgcc gactcggcga acgcatggtc ggcaccaccc
     1081 ttccgctcac gtcgcggagt caggccgcgg cggaggcggc g
//
LOCUS       DASPLT010000739         1587 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_152236, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000739 DASPLT010000000
VERSION     DASPLT010000739.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1587)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1587)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1587
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_152236"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..949
                     /locus_tag="VEA63_07735"
     CDS             <1..949
                     /locus_tag="VEA63_07735"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HYD83928.1"
                     /translation="VNPSWDILKTLSDPTRLRLLALLTREELSVAELQEILGMGQSRI
                     SSQLALLRQVNLVTDRRDGKKAFYSLRANLPAKTLALLKASIDSVSELSVVAEDRDNL
                     DRILQKRRRTQEQYFNLIAGRLGKNYCPGRSWEAIGHLALRLTPAIDIADLGAGEGLV
                     SQLLAARARQVWCIDNSPRMVEVGTELARKNNLVNLVYKLGDIEHVPLADKSVDLAIL
                     SQALHHAQHPQAAVDEAHRILRPGGQVLILDLNEHTFEKAHELYADVWLGFKESALHH
                     FLKKAGFTKVEVATVAKEPSEPFFETLLASGVKAGKPER"
     gene            1377..>1587
                     /locus_tag="VEA63_07740"
     CDS             1377..>1587
                     /locus_tag="VEA63_07740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD83929.1"
                     /translation="MNSTPLRVRPQLFPFLLTLVALGACSSPPGPPRVDNLPMYGAPE
                     VPRPTQLKQADERFVRNAAAGFRGDR"
BASE COUNT          296 a          599 c          391 g          301 t
ORIGIN      
        1 cgtgaatcca tcttgggaca tccttaaaac cctctccgac cccacgcgcc tccgcctcct
       61 cgcgctcctc acccgcgaag agctctccgt cgccgaactt caggaaatcc tcggcatggg
      121 ccagtcgcgc atctcctcgc agctcgcgct tctccgccag gtcaatctcg tcaccgaccg
      181 tcgcgacggc aaaaaagcct tctattccct gcgcgcgaat ctgcccgcga aaaccctcgc
      241 gctgctgaaa gcctcgatcg actccgtcag cgagctctcc gtcgtcgccg aagaccgcga
      301 caatctcgat cgcatcctcc aaaaacgccg ccgcacccag gagcagtatt tcaacctcat
      361 tgccggccgc ctcggcaaaa attactgccc cggccgctct tgggaggcga tcggccatct
      421 cgcgctccgc ctcacgcccg cgatcgatat cgccgacctc ggcgccggcg aaggcctcgt
      481 ctcccaactc ctcgccgcgc gcgcccgcca ggtctggtgc atcgacaact cgccgcgcat
      541 ggtcgaggtc ggcaccgagc tcgcgcgcaa aaacaacctc gtcaatctcg tctacaagct
      601 cggcgacatc gaacacgttc cgctcgccga caaatccgtc gaccttgcga tcctctcgca
      661 agcgctccac cacgcgcagc acccgcaggc cgccgtcgac gaagcgcacc gcattctccg
      721 ccccggcggc caggtcctga tcctcgacct caacgaacac accttcgaaa aagcgcacga
      781 gctctacgcc gatgtctggc tcggcttcaa ggaaagcgcc ctgcatcact tcttgaagaa
      841 agccggtttc acgaaggtcg aagtcgccac cgtcgcgaaa gaacccagcg agccgttctt
      901 cgaaacgctt ctcgccagcg gcgtcaaagc cggaaaaccc gagcgctaag ccggactcct
      961 acagaagaga aaccaccaat cgacacgaat gaacacgact tcagaggagc cgaaggacag
     1021 cttcgaggct gatcgttacg tcacctttcg gtgttccgat aaggctccgc attctaacgc
     1081 gatttcattc gtgcctattc gtggttcaaa tgaactgttc caactaagct cactcgcccc
     1141 gcagcgaaaa acgtaagttt ttcgtccacc aaagcggccg cttttcgccg gcccgaaccg
     1201 ctcgcttccg ccgcgctcct ttcgcctacg tatctttgcg cagttgcgcc tgctgattcg
     1261 ctttgcgctt tgtccgcttc ttcgtgcgat tgagccgtcc acttcgtcgc gccgccctgc
     1321 gcggatagcg cgaggccgct tgcgcgtcgc tcaacgagtc taatttttcc gtgtcgatga
     1381 actccacgcc ccttcgcgtc cgtccgcagc tttttccatt tctgctgact ctcgtcgcgc
     1441 tcggcgcctg ctcctcgccg cccgggccac cgcgcgttga caacctcccc atgtatggcg
     1501 cccccgaagt cccgcggccg acgcagttga agcaagccga cgagcgtttc gtgcgcaacg
     1561 ccgccgccgg cttccgtggc gaccgcc
//
LOCUS       DASPLT010000740         1607 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_110757, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000740 DASPLT010000000
VERSION     DASPLT010000740.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1607)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1607)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1607
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_110757"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(122..1378)
                     /locus_tag="VEA63_07745"
     CDS             complement(122..1378)
                     /locus_tag="VEA63_07745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HYD83930.1"
                     /translation="MAELVGNVLVGQSGGPTAVINASVAGIVAEALNHECIEEIYGAL
                     NGVLGILNEDLIDLAAESQQQIRALRHTPGAALGTCRYKLKKQSDYERVLEVFKAHNI
                     RYFFYIGGNDSQDTADKISKLAQQQAYELRVIGVPKTIDNDLPITDHTPGYGSVIKYT
                     ATTIRELACDHEAMGQGDLVSIVEVMGRSAGWIAAGAALAKRRDHPHDPPHLIYLPEI
                     AFDQAKCLEDIKRILKREKYCMIVVGEGLIDADGNYVSANAATDSFGHAQLGGAGDYL
                     KELVENNLPGVKARVAKPGIIQRAAAHAGSKTDSDEAFMAGQAAVEAAIDGQTDKMIT
                     LLRGDSDHYTCETGLAPLSDIANGVKKLPREWINEDGTSMNFQFLRYAQPLIQGEVTL
                     PYDNGVPVFARLDKVRVDKTLAAFAG"
     gene            complement(1363..>1607)
                     /locus_tag="VEA63_07750"
     CDS             complement(1363..>1607)
                     /locus_tag="VEA63_07750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD83931.1"
                     /translation="RSHGFQRRRVSYKRSFAARSDERRGPIVVITRPFAAPHDRAFSG
                     KLLRWKFEFASPLREPLSCPFTLFPTLYKHSSWLNS"
BASE COUNT          287 a          424 c          575 g          321 t
ORIGIN      
        1 cgacaggacg cgggctcgac ctacaagaaa ttcgccagcg aaaaggccgg tcacgaggac
       61 cggcctttcg gggagctccg catgctcacg catgcggcta caagttttcg attcgcgccg
      121 ttcagccagc gaaggccgcg agggttttat cgacgcggac tttgtcgagc cgcgcgaaaa
      181 ccgggacgcc gttatcgtaa ggaagggtga cttcgccctg gatgagcggc tgcgcgtagc
      241 ggaggaactg gaagttcatg ctcgtgccgt cttcgttgat ccactcgcgg ggaagtttct
      301 tcacgccgtt cgcgatgtcg ctgagcgggg cgagaccggt ctcgcaggtg taatgatcgc
      361 tgtcgccgcg gaggagcgtg atcatcttgt cggtctggcc atcgatcgcg gcttcgacgg
      421 cggcctggcc ggccatgaag gcttcgtcgg agtcggtttt cgagccggcg tgcgcggcgg
      481 cgcgctggat gatgccgggc ttggcgacgc gggctttgac gcccgggaga ttgttttcga
      541 cgagttcctt gaggtaatcg ccggcgccac cgagttgagc gtggccgaag gaatcggtcg
      601 cggcgttggc cgagacgtag ttgccgtcgg cgtcgatcaa gccttcgccg acgacgatca
      661 tgcagtattt ctcgcgcttc aggatgcgtt tgatgtcctc gaggcacttg gcttggtcga
      721 acgcgatctc ggggagataa atgaggtgcg gcggatcgtg cgggtgatcg cgacgtttcg
      781 cgagggcggc gccggcggcg atccagccgg cggaacgacc catgacttcg acaatggaaa
      841 cgagatcgcc ctggcccatc gcttcgtggt cgcacgcgag ttcacgaatc gtggtggcgg
      901 tgtatttgat cacgctgccg tagccgggcg tgtgatcggt gatcggaaga tcgttatcga
      961 tcgtcttcgg cacgccgatg acgcggagct cgtaggcctg ctgttgggcg agcttggaaa
     1021 tcttgtccgc ggtgtcctgg gaatcgttgc cgccgatgta gaaaaagtag cggatgttgt
     1081 gcgctttgaa gacctcgagc acgcgttcgt agtcggattg cttcttgagc ttgtagcggc
     1141 aggtgccgag agcggcgccg ggcgtatggc ggagagcgcg gatttgctgc tgggactcgg
     1201 cggcgagatc gatcaggtcc tcgttgagga tgccgagcac gccgttcagc gcgccgtaga
     1261 tttcctcgat gcactcgtgg ttgagcgcct cggccacgat gcccgcgaca ctggcgttga
     1321 tgacggcggt ggggccaccg gactggccca ccaagacatt tcctacgagt tcagccatga
     1381 cgaatgtttg taaagggttg gaaacaacgt gaacgggcaa gaaagcggct cgcgaagggg
     1441 ggatgcaaac tcaaatttcc agcggaggag tttcccggaa aaggcccgat catgaggtgc
     1501 ggcgaagggg cgtgtaatga cgacaattgg cccgcggcgc tcgtcagagc gggcggcaaa
     1561 tgagcgttta taggagacgc ggcggcgctg gaagccatgc gagcggg
//
LOCUS       DASPLT010000741         1054 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_152266, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000741 DASPLT010000000
VERSION     DASPLT010000741.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1054)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1054)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1054
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_152266"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..465)
                     /locus_tag="VEA63_07755"
     CDS             complement(<1..465)
                     /locus_tag="VEA63_07755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HYD83932.1"
                     /translation="MKPIRKLAFVINGDKAGASDLARELITAARAAGVKLKHTVRFPL
                     PAGYLRGFDACCVIGGDGTLLGVAREAAHQQVPIIGVNRGSLGFLTTFSADEARSHFV
                     ELLNGQYLIAQRSLLECCTGPNSHDLALNDVLVKSDVNSRLVQLEVHADGELV"
     gene            complement(714..>1054)
                     /locus_tag="VEA63_07760"
     CDS             complement(714..>1054)
                     /locus_tag="VEA63_07760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HYD83933.1"
                     /translation="ADLPRADYDAIVMDLSFISLKSVLPAVWPLLRDGGALVALVKPQ
                     FEAGKAEVDKGRGVIRDAAVQDAALANVRDFALAQLPAAQLVGTTDSPITGADGNREF
                     LLCLRKGPPA"
BASE COUNT          206 a          298 c          366 g          184 t
ORIGIN      
        1 gacgagctcg ccgtcggcgt gcacttcgag ttggacgagc cgcgagttga cgtcgctctt
       61 cacgagcacg tcgttgagcg cgaggtcgtg cgagttgggg ccggtgcagc actcgaggag
      121 tgagcgttgc gcgatgaggt attggccatt gagcagctcg acgaaatgcg agcgcgcttc
      181 gtcggcggaa aacgtcgtga ggaagccgag gctgccgcga ttcacgccga tgatcggcac
      241 ctgttggtgg gcggcttcgc gcgcgacgcc gaggagagtg ccgtcgccac cgatgacgca
      301 gcacgcgtcg aagccccgca gataaccggc cgggagcgga aagcggacag tgtgcttcag
      361 tttcacgccg gcggcgcgtg cggcggtgat caactcgcgc gcgagatcgg aagcgccggc
      421 cttgtcgcca ttgatgacga acgcgagttt gcggatgggc ttcatgcgag cggttagtga
      481 aaccacgaac ggacgcgaat cgacatgaat atcacggcgg cgacggtctt cgtgtccact
      541 tggggtgaaa aattttcaac cacggatgaa cgcggattca atcgccgagc cttgtggctg
      601 tgcgctaacc gagaagggag aaccacgaat ggacacgaat tcacacgaat aacgaaccga
      661 tcatgtttct cgtgtccgtt ggcggtcgtt cgtgattcga atgaatcgtt gcattaagcg
      721 ggcggacctt ttcggaggca caggagaaac tcgcggttgc cgtcggcgcc ggtgatcggc
      781 gagtccgtgg tgccgacgag ttgcgcagcg gggagctgcg ccagagcaaa gtcgcgcacg
      841 ttcgcgagcg cggcgtcctg gactgcggcg tcgcggatca cgccgcggcc tttgtcgacc
      901 tcggctttgc ccgcttcgaa ttgcggcttc accagcgcga cgagcgcgcc gccgtcacgc
      961 aacagcggcc agacggcagg caacacggat ttcaacgaga tgaacgacag gtccatcacg
     1021 atcgcgtcgt aatcggcacg cggcagatcg gcgg
//
LOCUS       DASPLT010000742         1538 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_274552, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000742 DASPLT010000000
VERSION     DASPLT010000742.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1538)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1538)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1538
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_274552"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..816)
                     /locus_tag="VEA63_07765"
     CDS             complement(<1..816)
                     /locus_tag="VEA63_07765"
                     /inference="COORDINATES: protein motif:HMM:NF020037.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HYD83934.1"
                     /translation="MPDFQKVFETSPDAYLVLDADPTFTIAAVNDAYLRATMTTREQI
                     VGRPIFEVFPDNPEQPHGVSVKTLRASLETVVATRQRHEFPPLQYDIPRSAAQGGGFE
                     QRFWRTSNIPVLGADGSVRQIIHRVEDITALVRLQQTERDHQRDNEALLARAAAARAE
                     VESRNEELAQTHRDLAEHRQLLHEARVRLEAAVSAGDIATFIWELGTDSIHADQNLRA
                     WFRLPADAPAKAALSHYRAAFHPDDLPKVQHVVARIRAGEAPAGIEFRLRNGDS"
     gene            1153..1452
                     /locus_tag="VEA63_07770"
     CDS             1153..1452
                     /locus_tag="VEA63_07770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dabb family protein"
                     /protein_id="HYD83935.1"
                     /translation="MLVHTVYFWLKPELSSAERAEFRRGVESLAAIAAVEKIYVGTPA
                     ATPKRPVIDDTYSVALTVICKDIAAQNEYQIDPVHLEFVEKFKTYWTRVQIYDAE"
BASE COUNT          251 a          371 c          630 g          286 t
ORIGIN      
        1 ggagtcgccg ttgcgcagcc ggaattcgat gccggcgggc gcctcgccgg cgcggatgcg
       61 cgcgacgacg tgttgcacct tgggcaagtc gtcgggatgg aaggcggcgc ggtagtggct
      121 gagcgcggct ttcgcgggag cgtcggccgg caggcgaaac cacgcgcgaa gattttggtc
      181 ggcgtgaatc gagtcggtgc cgagttccca aatgaacgtg gcgatatcgc cggcgctgac
      241 ggcggcctcg aggcgaacgc gtgcctcgtg gaggagctgg cggtgttccg cgagatcgcg
      301 gtgagtttgc gcgagctctt cgttgcggga ttcgacttcg gcgcgcgcgg cggcggcgcg
      361 tgcgagcagc gcctcgttgt cgcgctggtg gtctcgctcg gtttgctgca ggcggacgag
      421 ggcggtgata tcctcgacgc gatggatgat ttgacgaacg gagccgtcgg cgccgaggac
      481 gggaatgttc gatgtgcgcc agaagcgctg ctcgaagccg ccgccttggg ccgcggagcg
      541 cggaatgtcg tattggagcg gagggaattc gtgacgctgg cgcgtggcga cgacagtctc
      601 gagggaggcg cgcagcgttt tcacggacac gccgtgaggt tgttcggggt tgtcaggaaa
      661 cacctcgaaa atcggacgac cgacgatctg ctcgcgggtc gtcatcgttg cgcgcaggta
      721 agcgtcgttg acggcggcga tcgtgaacgt cggatcggcg tcgaggacaa ggtaagcgtc
      781 gggcgaagtt tcgaaaactt tttggaagtc cggcatgcga tcggcgcggg gcgctgcgtt
      841 ttcgagggcg ttcaggcgcg gaggttcctg agtcagggga atgcgtggag gtggagcggg
      901 gtgggggttt tgtaaaggcc caccggtgtg cgtttagaac cgctgtccgg attccgccga
      961 ggaggagagc gggtaggttt ccgatggggc taagggaaaa ggcggcagcg tgcggtggcg
     1021 gcagcgtgcg tggcacgcgg tgtgagtgaa ggtgggtgcg acaacgcggt caggttgacc
     1081 gcgctacgga atgggtgggt tgcggaggtt cgcgcttgcg ccgggacggg aggcgggaag
     1141 gctcggcgac ccatgttggt gcacaccgtt tatttctggc tgaaacctga attgtcgtcg
     1201 gcggaacgcg cggaattccg ccgcggcgtc gagtcgctcg ccgcgatcgc tgcggtggag
     1261 aaaatttatg tcggcacgcc ggcggcgacg ccgaagcggc cggtgatcga cgacacgtat
     1321 tcggtggcgt tgacggtgat ctgcaaagat atcgcggcgc agaacgaata tcagattgat
     1381 ccggtgcacc tggagttcgt cgagaagttc aagacgtatt ggacgcgcgt gcagatttac
     1441 gacgcggagt gaagagggag tgagggaatg ggggagtgag ggagtggggc ggcgcggggg
     1501 cggtgggcgt cgggtctgtt tgcctgcggt gtaggttg
//
LOCUS       DASPLT010000743         1605 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_194119, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000743 DASPLT010000000
VERSION     DASPLT010000743.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1605)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1605)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1605
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_194119"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(68..1300)
                     /locus_tag="VEA63_07775"
     CDS             complement(68..1300)
                     /locus_tag="VEA63_07775"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="vanadium-dependent haloperoxidase"
                     /protein_id="HYD83936.1"
                     /translation="MLALAFFVTAPLASANSVLEWNEQALNATRLARNPPPPASYFLA
                     VYHLAIFDAVNGITRTHESWHVNEPAPAGADLDAAVAGAAHTAMAALWSSTNPRVVQN
                     FYEKALAAIPDGQPKTDGLAWGKHVAEKILERCSDSGYNKPIPGVYSSNEPGKWRETP
                     AAFRPPLMPFWAKVRPFAMTSPNQFRAPPPHPVDSKEHAEELAFVAKVGPRDGAERTE
                     YETMSTPFWSDDLGTSTPPGHWNMVAQDLARRNKLTVPETARLLALVNIAQADAAIAC
                     WETKFFYNTWRPETGIRELDSKLNPHAKPSPDFIPNLASPSFPSYTSGHSTFSGAGAR
                     SLALFFGTDEIEFTLTSDGLPGAVRTYKRLSDAQREAGMSRVWGGIHVMSDNLEGQKA
                     GVKIAEWTFANALKPLAK"
     gene            1329..>1605
                     /locus_tag="VEA63_07780"
     CDS             1329..>1605
                     /locus_tag="VEA63_07780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD83937.1"
                     /translation="MRGGITCVETGRCSRLGKGTNGECGGTKQGKLLWGELRESRGEP
                     RDFWAIGWAQALAERENHVGDLLDDFEAVAARHVDVEKHDVGLLATNC"
BASE COUNT          282 a          409 c          630 g          284 t
ORIGIN      
        1 tggcgcggtg gggatgggag ataggcgcag agctcgaaga acggtggagc gcggcccgcg
       61 cggcggatta tttggcgagc ggcttgagcg cgttggcgaa ggtccactcg gcgatcttca
      121 cgccggcttt ctggccttcg aggttgtcgg acatgacgtg gatgccgccc cacacgcggc
      181 tcatgccggc ttcgcgctgc gcgtccgaga ggcgtttgta agtgcggacg gcgccgggca
      241 ggccatccga agtgagggtg aattcaatct cgtcggtgcc gaagaacaac gcgaggctgc
      301 gggcgccggc gccggaaaaa gtgctgtggc cggacgtgta ggacgggaac gacggcgagg
      361 cgaggttggg aatgaaatcg gggctcggct tggcgtgggg attcagtttc gagtcgagct
      421 cgcggatgcc ggtttcgggg cgccaggtgt tgtagaaaaa tttcgtctcc cagcacgcga
      481 tggcggcgtc ggcttgcgcg atgttgacga gggcgaggag tcgcgcggtt tccgggacgg
      541 tgagtttgtt tcggcgggcg aggtcctgcg cgaccatgtt ccaatgccca ggtggcgtcg
      601 acgtgccgag atcgtcggac caaaagggcg tgctcatggt ttcatattcc gtgcgctcgg
      661 cgccgtcgcg cgggccgact ttggcgacga aggccagctc ctcggcgtgt tctttcgagt
      721 cgaccggatg cggcggggga gcgcgaaatt ggttgggcga cgtcatcgcg aacggacgaa
      781 ccttggccca gaagggcatg agcggcgggc ggaaagcggc gggcgtttcg cgccatttgc
      841 cgggttcgtt gctcgaatac actccgggaa tcggcttgtt gtagccgctg tcggaacagc
      901 gctcgaggat cttctcggca acgtgcttgc cccacgcgag gccgtcggtt ttgggctggc
      961 cgtccggaat ggcagcgagc gccttctcat aaaaattctg aacgacgcgc gggttggtgg
     1021 aactccacag tgcagccatc gcggtgtgcg cggcgccggc gaccgcagca tcgagatcgg
     1081 cgccggcggg cgcgggttcg ttgacgtgcc agctctcgtg cgtgcgagtg atgccgttga
     1141 cggcgtcgaa gatcgcgagg tggtaaacgg cgaggaagta ggaggccggt ggcgggggat
     1201 tgcgcgcgag ccgggtggcg ttcagcgctt gctcgttcca ttcgagcacg gaattggccg
     1261 aggcgagcgg agcggttacg aaaaaggcga gggcgagcaa gggggacgac aggaagcgag
     1321 gggaacgcat gaggggtggc ataacgtgtg ttgaaaccgg tcgatgcagc cggctgggga
     1381 aggggacgaa cggagaatgt gggggaacga agcaaggcaa actgctttgg ggtgaactgc
     1441 gggaatcgcg gggtgaaccg cgcgattttt gggccatcgg ctgggcgcag gcgctcgcag
     1501 aacgagaaaa ccacgtcggc gatttgctcg acgacttcga agccgttgca gcgcggcatg
     1561 tggacgtcga gaaacacgat gtcgggctgc tcgcgacgaa ttgcg
//
LOCUS       DASPLT010000744         1344 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_9576, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000744 DASPLT010000000
VERSION     DASPLT010000744.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1344)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1344)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1344
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9576"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1167
                     /locus_tag="VEA63_07785"
     CDS             <1..1167
                     /locus_tag="VEA63_07785"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HYD83938.1"
                     /translation="TLLKIFAADGSQVAIGDPLCAIGKPGEKIDAPAPKSAAPAPAQS
                     PADDAKVSAPGASTTSKNEVEARPTTEPKADAKAAAASAPAQKREGGRIKISPLARKL
                     AEEKGIDPADVTGSGPGGRIVRADIEAASKAPRSEIANRKSQIGALGAKGPVQEERTV
                     PVSNMRGAIARRLVESKTQYPHFYVEVEIDAAPLLALREQLNSGLEKEVVKLSVNDFI
                     LKASAEALRRVPQVNASWETAASGQGQIRYFGAAHVAFAVAMEDGLITPVIRDAHQKS
                     VFQISTEAKSLGKLAKDKKLKPDQFTGGTFCVSNMGMMGVQRFSAIINPPNAAILAVG
                     TTVKKPVVKNDQIVIGQAMVLTLSCDHRVVDGAVGAQYLAALKQILEAPALLLV"
BASE COUNT          267 a          482 c          384 g          211 t
ORIGIN      
        1 acgctgttga agatcttcgc cgccgacggt tcgcaagtcg ccatcggcga tccgctctgc
       61 gcgatcggca aacccggtga aaaaatcgac gcccccgcgc cgaaatccgc cgcgcccgcg
      121 cccgcgcaat cgcccgccga cgacgcgaag gtctccgcac ccggcgcttc aacgacgtcg
      181 aaaaacgagg tcgaagcacg tcccacgaca gagccgaaag cggacgccaa agctgccgcc
      241 gcgtcagcgc ccgcgcaaaa acgcgaaggc ggtcgcatca agatttcccc gctcgcccgc
      301 aagctcgcgg aagaaaaagg catcgatccc gccgacgtca ccggctccgg tcccggcggt
      361 cgcatcgttc gcgccgacat cgaggcagcg tcgaaggcgc cgcgttctga aatcgcaaat
      421 cgcaaatcgc aaatcggcgc cctcggcgcc aagggccccg tgcaggaaga gcgcacggtt
      481 cctgtttcaa acatgcgcgg cgcgattgcc cgccggctcg tcgaatcgaa gacgcagtat
      541 ccgcatttct acgtcgaagt cgaaatcgac gccgccccgc tcctcgcttt gcgcgagcag
      601 ctcaacagcg gcctcgagaa ggaagtcgtt aaactctccg tcaacgactt catcctgaaa
      661 gccagcgccg aagcgctccg tcgcgtgccg caagtcaacg cctcctggga aaccgccgct
      721 tccggccagg gccagatccg ctacttcggg gccgcgcacg tcgctttcgc cgtcgccatg
      781 gaagacggcc tcatcacacc ggtgatccgc gacgcgcacc agaaaagtgt tttccaaatc
      841 agcactgaag cgaagtcgct cggcaaactg gccaaggaca aaaagctcaa gcccgatcag
      901 ttcaccggcg gcacgttctg tgtgtcgaac atgggcatga tgggcgtgca gcgtttcagc
      961 gcgatcatca acccgcccaa tgccgcgatc ctcgccgtcg gcacgaccgt gaaaaagccg
     1021 gttgtgaaaa acgaccagat cgtgatcggc caggcgatgg tgctcacgct ctcctgcgac
     1081 caccgcgtcg tcgatggcgc cgtcggcgcg caatacctcg ccgcgctcaa gcagatcctc
     1141 gaagcccccg ccctgctgct ggtctaaact tcccgcgtag cggcaggcct ccgtgcctgc
     1201 ctgctccgac gccgcgccga ggcctgcacg tatccgccgc gctttcgcgt gcggcgattc
     1261 cgctcggcgc gatgctgaac gctcacgcat tccgctccga cagacatttg cgtgttcggt
     1321 agcggcgatc atctgttcgc cgct
//
LOCUS       DASPLT010000745         1105 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_194170, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000745 DASPLT010000000
VERSION     DASPLT010000745.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1105)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1105)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1105
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_194170"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1089)
                     /locus_tag="VEA63_07790"
     CDS             complement(<1..1089)
                     /locus_tag="VEA63_07790"
                     /inference="COORDINATES: protein motif:HMM:NF018647.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046556 - alpha-L-arabinofuranosidase
                     activity [Evidence IEA];
                     GO_process: GO:0046373 - L-arabinose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-arabinofuranosidase C-terminal
                     domain-containing protein"
                     /protein_id="HYD83939.1"
                     /translation="MPSDNLKGMRRDTLALLKQLNGTIYRWPGGNFVSGYDWRDGIGD
                     RDRRPPRKNPAWTGVEHNDFGTDEFIVFCREIGTEPMIAANTGMGGAYSAAQWVEYTN
                     RGADTIGGGMRAKNGNREPYNVKYWCVGNEMWGTFQLGWMQLHHYTIKHNLVAEAMWK
                     ADPKLELTAVGDLETINRNHDPEAVKNNLPWSKGMLQSCADYMDSISEHFYDGRLPWN
                     QAGRVGVVEHAGLIKKNIRKKVDGHRELQPTLANLKGRRIPISMDEWNYWHREYAYGE
                     LGCIYELQDGLGIAAGLHEFYRNSDFVPMAFYAQTVNVIGAIKTTRTAAEMETTGLVL
                     QMYRAHFGEMPLQLEQNFGDRDVAAALSK"
BASE COUNT          190 a          328 c          343 g          244 t
ORIGIN      
        1 tttcgaaagc gccgccgcta catcgcgatc gccgaaattt tgttcgagct gcaacggcat
       61 ctcgccgaaa tgcgcccgat acatctgcag caccaaaccg gtcgtttcca tttctgcggc
      121 ggtgcgcgtc gtcttgatcg caccaatgac gttcaccgtc tgggcgtaga acgccatggg
      181 aacgaaatcg ctgttgcgat aaaactcgtg cagaccggcc gcgataccga ggccgtcctg
      241 cagctcataa atgcagccga gttcgccgta agcgtattcg cggtgccagt aattccactc
      301 gtccatcgaa atcgggattc tgcggccttt gagattcgcg agcgtcggct ggagctcacg
      361 gtgaccgtcg actttcttgc ggatgttttt cttgatgagt cccgcgtgct cgacgacgcc
      421 gacgcgtcca gcctggttcc atggcaagcg accgtcgtag aagtgctcgg aaatcgaatc
      481 catgtaatcg gcgcaggatt ggagcatgcc tttcgaccaa ggcaggttgt tcttcacggc
      541 ttctggatcg tgattgcgat tgatcgtctc gagatctccc accgcggtca gctcgagctt
      601 cgggtcggcc ttccacatgg cctcggcgac caggttgtgt ttgatcgtgt aatgatggag
      661 ctgcatccag ccgagttgga acgtgcccca catctcgttg ccgacgcacc agtatttgac
      721 gttgtagggc tcgcgattgc cgttcttcgc gcgcatgccg ccgccgatcg tgtcggcgcc
      781 gcggttggtg tattcgaccc actgcgcggc ggagtaggcg ccgcccatgc cggtgttggc
      841 cgcgatcatg ggctcggtgc cgatctcgcg gcagaaaacg atgaactcgt ccgtgccgaa
      901 gtcgttgtgc tcgacgccgg tccacgcggg attcttgcgc ggcggacggc ggtcgcggtc
      961 gccgatgccg tcacgccagt cgtaaccgct gacgaagttg ccgcccggcc agcgataaat
     1021 cgtgccgttg agctgtttca acagcgcgag ggtgtcgcga cgcatgcctt tcaggttgtc
     1081 ggaaggcatg agcgagggcg ggccg
//
LOCUS       DASPLT010000746         1172 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_296012, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000746 DASPLT010000000
VERSION     DASPLT010000746.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1172)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1172)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1172
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_296012"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(222..>1172)
                     /locus_tag="VEA63_07795"
     CDS             complement(222..>1172)
                     /locus_tag="VEA63_07795"
                     /inference="COORDINATES: protein motif:HMM:NF014683.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015129 - lactate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015727 - lactate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HYD83940.1"
                     /translation="IGPMLVDIISAVVSIGALVVLMKFWRPQKTDTAGEKIPAAAAVA
                     SAGVGLPAAGVMATSAPSPARAWLPWIFLSVFVFAWGLPVIKNALNALFAPSFAMPGL
                     HQLVQRMPPVVPHPELENAIFSFNFLSATGTALLFAGIVAGFCLGLTPGKLAALYGRT
                     LYRVRVSLLTIALMLALGFTTRYSGTDTTMGLAMAATGMFYPFFSPIIGWLGVALTGS
                     DTSSNVLFGNLQKVTAQQLGFSPILMAASNSSGGVMGKMIDAQSIVVAAVATGGRPDS
                     PDAGTVLRSVFWHSVALALLMGALVTFQTYVWTSVVPGAR"
BASE COUNT          249 a          394 c          360 g          169 t
ORIGIN      
        1 catcactcgg cgcgcaaaag gcgcaagccg tcctcgcaac gggcgcctcg atcgtcgcga
       61 gcggaaacat cggctgcctc acgcaactcc gcacgcactt ggaaaaactc ggttcgccca
      121 tccgcgtccg ccacacgatg caagtcctgc gcgacgcgta tgaggaatag actgggtatc
      181 gcatggtagg cgaggcgccc cccgagccgc agcgcgacgg cttaccgtgc gcccggcacc
      241 acgctcgtcc acacataggt ctggaacgtc accagcgcgc ccatcagcaa tgcgagtgcg
      301 acgctgtgcc agaacaccga gcgcaggacc gtgcccgcat ccggactgtc gggccgtccg
      361 ccggtcgcca ccgcagcgac cacgatgctt tgcgcgtcga tcatcttgcc catcactccg
      421 ccggagctgt tcgacgccgc catcaggatc ggcgagaaac cgagttgttg cgcggtgact
      481 ttttggaggt tgccgaaaag cacgttcgac gacgtgtcgc tgccggtgag cgcgacgccg
      541 agccagccga taatcggcga gaagaacgga taaaacattc cggtggccgc catcgccagc
      601 cccatcgtcg tatccgttcc actgtagcgg gtcgtgaaac cgagcgcgag catcagcgca
      661 atcgtcagca gcgaaacgcg cacgcgatac agcgtgcgcc catacagcgc tgccagcttg
      721 ccaggcgtca gcccaaggca gaaacccgcg acaatccccg cgaacaaaag cgccgtgccg
      781 gtcgccgaaa gaaagttgaa actgaagatc gcattctcga gctcgggatg cggcaccacg
      841 ggcggcatcc gctgcacgag ctggtgcagc ccgggcatcg cgaacgacgg cgcgaaaagc
      901 gcattcagcg cgtttttgat cactggcaat ccccaggcga agacgaagac ggaaagaaat
      961 atccacggca gccacgcgcg cgccggcgag ggagccgatg tcgccatcac cccggcggcg
     1021 ggcagcccga caccggctga ggcgacggcg gcggcggcgg gaattttttc gccggccgta
     1081 tccgtttttt gcggacgcca gaacttcatc aacacgacca gcgcgccaat cgatacgacc
     1141 gcgctgatga tgtcgacgag catgggtccg at
//
LOCUS       DASPLT010000747         1675 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_132461, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000747 DASPLT010000000
VERSION     DASPLT010000747.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1675)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1675)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1675
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_132461"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..404)
                     /locus_tag="VEA63_07800"
     CDS             complement(<1..404)
                     /locus_tag="VEA63_07800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007361738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HYD83941.1"
                     /translation="MSFRKVLFWLHLVAGLVAGLSIGIMCFTGVVLAYEKQIVSWSER
                     DARRVPAPTAGQTRLSLDELTARVRTAHPDASPASIIVSADPRDAVTFSLGRDNTVFA
                     NPYTGAIQKPASTAVHDFMHVMEDWHRVLAFAG"
     gene            complement(508..993)
                     /locus_tag="VEA63_07805"
     CDS             complement(508..993)
                     /locus_tag="VEA63_07805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD83942.1"
                     /translation="MRTSPTVPTLSFPLPLSPSAAEACPLNHGVAVVDQLLAAITRAS
                     EFDAHALDVALDFRLLLASSTDAEDILQAFFRVRAAVEENHYLACFRLRRWLEIQFAA
                     HVRPDRTQPIREVPVRLTANSYRELCARCAFDAAGNELVSPWVRVHFVTRQPHAVATS
                     R"
     gene            complement(1137..1622)
                     /locus_tag="VEA63_07810"
     CDS             complement(1137..1622)
                     /locus_tag="VEA63_07810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007363459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HYD83943.1"
                     /translation="MKPLAASTTKSNKTDSPIVSALNLLLADSYALMALTHHAHWNVE
                     GPGFFALHKAFEEQYEHLFRAVDDIAERVRALDSYALGGLRAFAKEAGMEEFATGAIP
                     AKDYVASLIIAHEKTLNDALAVRDAAGHANDLETQDLAIERITWHQKTTWMLRSYLKS
                     I"
BASE COUNT          322 a          390 c          674 g          289 t
ORIGIN      
        1 ccggcgaagg cgaggacgcg gtgccagtct tccatcacgt gcatgaaatc atggacggcg
       61 gtcgaggcgg gtttttggat cgcgccggtg taggggttgg cgaaaaccgt gttgtcgcgg
      121 ccgagggaaa acgtgacggc atcgcggggg tcggcggaaa cgatgatcga cgcgggagac
      181 gcgtccgggt gcgcggtgcg aacgcgtgcg gtcaattcgt cgagcgacag gcgcgtttga
      241 ccggcggtcg gagcggggac gcggcgggcg tcgcgttcgg accacgagac gatttgtttc
      301 tcgtaggcga gaaccacgcc ggtgaagcac atgatgccga tcgagagacc cgcgacgagg
      361 ccggcgacga ggtgaagcca aaaaaggact ttgcggaagg acatggaaga gggcgtgcgg
      421 ggcgcgggcg acgttgggcg cggagggcgg gcaggggcgg agaagcagga gagggcggcg
      481 tttagcggac gcctcggaga ggcgtcccta cctggacgtg gccacggcgt ggggttggcg
      541 ggtgacgaag tggacgcgga cccacggtga gaccaactcg tttccggcgg cgtcgaacgc
      601 gcatcgggcg cagagctcgc gatagctgtt agccgtcagg cgaaccggaa cctcgcggat
      661 cggttgagtg cggtcgggcc ggacgtgcgc ggcgaactga atctcgagcc agcgacgaag
      721 gcggaagcag gcgaggtagt ggttttcttc gacggcagca cggacgcgga agaacgcttg
      781 aaggatgtct tcggcatcgg tcgaactcgc gagcaacagg cggaaatcga gggcgacgtc
      841 gagagcgtgg gcgtcgaact cggatgcgcg cgtgatggcg gcgaggagtt gatcgacgac
      901 ggcgacgccg tgattaagcg ggcaggcctc tgcggcggac ggggagagcg gaagcgggaa
      961 agaaagcgtc ggaacggtcg gggaagttct cattaggagt gaaactcagt ctcagcagag
     1021 gggctttaaa aagcgccgac cggtgtcggc gcagaaattt ttgagggatt gtaagcttgt
     1081 cgatacgatc ggccgggcga gggcgcgcgg ccgagggtgt gttgatggaa agtgagttag
     1141 atggatttaa ggtagctgcg tagcatccaa gtggtctttt ggtgccaggt gatacgctcg
     1201 atggcaagat cctgtgtttc gagatcgttg gcgtggccgg cggcgtcgcg gacggcgagg
     1261 gcgtcgttga gcgtcttttc gtgcgcaatg atgagggacg cgacgtagtc cttggcgggg
     1321 atggcgccgg tcgcgaactc ttccatgcca gcttccttgg cgaaggcgcg gaggccgccg
     1381 agcgcgtagg aatcgagggc gcgaacgcgt tcggcgatgt cgtcgacggc gcggaaaagg
     1441 tgttcgtatt gttcttcgaa cgctttgtga agggcgaaga agccggggcc ttcgacgttc
     1501 caatgcgcgt gatgggtgag cgccatcagc gcgtaagagt cggcgaggag aagattcagt
     1561 gccgagacga tcggcgaatc ggttttgttg gatttggtgg tggaggcagc gagcggtttc
     1621 atggcgaagg gagtctgttg ggttgcgctg agttagcaac tccgcgcgtg gcaat
//
LOCUS       DASPLT010000748         1440 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_152398, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000748 DASPLT010000000
VERSION     DASPLT010000748.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1440)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1440)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1440
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_152398"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(522..>1440)
                     /locus_tag="VEA63_07815"
     CDS             complement(522..>1440)
                     /locus_tag="VEA63_07815"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HYD83944.1"
                     /translation="AYSDVAYAQQFFRIAHRSRELAATLLDENEKRFSVGSMSESDVT
                     QARARMATRDETILFAEQAVRDTVNRLRQLIGETHFPVNPENIIVEAPEIPDDIAINP
                     AEDLRRAFEDRPDYQAARLGIVKRRANHAAARNELLPQIDLVGSYGYSGLDRDFAASR
                     RMVRDYENRSYSAGVVVSIPLTFSEGRGRARSAKLQVRQAESDLERLEQDIAVSIASA
                     AGQIETTRRRVAATRAAYTLAKEALDAELKKLRAGTSSTFFVLNLQGELIGAENSLHN
                     ALADQRRAHAAYDREVGRTLAVHHITLSE"
BASE COUNT          241 a          393 c          517 g          289 t
ORIGIN      
        1 gtccgagcgc gcttggcgcg ctgaccttta gttcgcggcg ccgggccgct ccgggcagat
       61 atacaacgca ccgatcgccg ccagttgctg gcgggcgcag aaaatataat tctgatgcgt
      121 gttcgggttc accgtggggc acgtgtgctt cgggaaaata tattgtcaag ctttgtgccg
      181 ccggcgatgc gcagagcgcg caagacggcg tggtcgggcc agcgcttgtg ttggactgac
      241 gcagactagc caacgatcgc cggcgcgatg aggatcaatc cggcgatcgg gcgaggcgag
      301 aacactgacg gataatgatc gtcggagagc gacggataac gtcgatggcg gaggccggcg
      361 ggctgaggtg agtgtctggg cgcagcacca cggtgaaatg ggagctgcga gttgggtggc
      421 gccgcggcag gatcatcgaa ccggttcgtg agttggtgaa agcgggcgtg tgaactcgaa
      481 agcagattcc accagccggc tttccttccc tcggcgtggt tctattccga cagcgtgatg
      541 tggtggacgg cgagcgtgcg gccgacttcg cggtcgtagg cggcgtgagc gcggcgttga
      601 tcggcgaggg cgttgtggag gctgttttcg gcgccgatga gttcgccttg cagattcaag
      661 acgaagaacg tgctgctggt gcctgcgcgc agtttcttca gctcggcatc aagtgcttcc
      721 ttcgcgagcg tataggcggc tcgcgtggcg gcgacgcggc gacgcgtggt ctcgatttga
      781 ccggcggcgc tcgcgatgct cacggcaatg tcctgttcaa gacgctcgag atcggattcg
      841 gcttggcgga cctgtaattt cgccgaacgc gcgcggcccc ggccctcgga aaaggtgagc
      901 gggatgctga ccaccacgcc ggcggagtag gaccgattct cataatcgcg caccatgcgt
      961 ctgctcgccg cgaaatcgcg atccagtccg ctgtagccat agctgccgac gagatcgatt
     1021 tgcgggagca gctcgttgcg tgcggcggcg tgattcgcgc gacgttttac gatgccgagt
     1081 cgcgctgcct ggtaatcggg ccgatcctcg aaggcgcgtc gcagatcctc ggccggattg
     1141 atcgcgatat cgtccggaat ctctggggct tcgacgatga tattctccgg gttcacgggg
     1201 aaatgggttt cgccgatgag ctggcgcagg cgattgacgg tgtcgcggac ggcttgttcg
     1261 gcgaacagga tcgtttcgtc acgggtggcc atgcgggcgc gcgcctgggt gacgtcgctt
     1321 tccgacatgc tgccgacgct gaagcgtttt tcgttttcat cgaggagcgt ggcggcgagc
     1381 tcgcgcgagc ggtgcgcgat ccggaaaaac tgctgcgcgt aggcaacgtc gctataggca
//
LOCUS       DASPLT010000749         1064 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_48787, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000749 DASPLT010000000
VERSION     DASPLT010000749.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1064)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1064)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1064
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_48787"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1064)
                     /locus_tag="VEA63_07820"
     CDS             complement(<1..>1064)
                     /locus_tag="VEA63_07820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD83945.1"
                     /translation="ALFDGARFDTSAKANELRTAMLQFWRDFRQECPELLVETRGTNL
                     SSGVDLSSDGVPLKEIYRDFGIVAPPNSPWAAINGDLGLELMGWMSHIAELPATGAFP
                     FRFYIHDPWFLNSPWLDRYCRQPHDIYLPLSVARLDREGEVQDPDSIEFLTIDDSRGR
                     MPDQVPLEVIPHIREAWRTRPDEAGPFTWIYPFDLYHSFTFEQHARIGEPFFGDWIVR
                     NALNDGLPLNTVMSEQNFVSLKSRDATPLANAVLVTHAPDAGSEMAEALIQHVTSGGK
                     AILYGPLEHTDPQLRSALGVTIGDALENDLQVTLSPKARRHADDAAYATRLVHETHLS
                     GGGLREVVQRSQDQNVEVLA"
BASE COUNT          204 a          300 c          364 g          196 t
ORIGIN      
        1 tgcgaggact tcgacgtttt ggtcttggga acgttgaaca acctcgcgca gcccgccgcc
       61 ggagagatga gtttcgtgaa cgagtcgagt cgcgtaggcg gcatcgtctg catggcgccg
      121 ggctttcggc gagagagtca cctgcagatc gttttccagc gcgtcgccga tcgtcacgcc
      181 gagcgcggag cgaagctgcg gatccgtgtg ctcgagcggt ccgtagagga tcgcttttcc
      241 gccagacgtc acatgttgaa tcagcgcttc ggccatttcg ctgccggcgt ccggcgcgtg
      301 ggtcacgaga accgcgttgg cgagaggagt ggcgtcgcgg cttttgagcg aaacgaagtt
      361 ttgttcgctc atgacggtgt tgagcggcag gccgtcgttc agggcgttgc gaacgatcca
      421 gtcgccgaag aacggttctc cgatgcgcgc gtgctgctcg aacgtgaacg agtgatagag
      481 atcgaaagga taaatccagg taaacggacc cgcttcgtcg gggcgcgtcc gccacgcttc
      541 acggatgtgc ggaatgacct cgagcggaac ctggtccggc atgcgtccgc gtgagtcgtc
      601 gatcgtgagg aactcgatcg aatcgggatc ctgcacctcg ccttcacgat cgagccgagc
      661 gaccgaaagc ggcaggtaga tgtcgtgcgg ctgtcggcag taacgatcga gccaggggct
      721 gttgagaaac cacggatcgt ggatgtagaa gcgaaaagga aacgcgccgg ttgcgggcag
      781 ttcggcgatg tgcgacatcc agcccatcaa ctccaggccg aggtcgccgt tgatcgcggc
      841 ccacggggaa ttcggcggag cgacgatgcc gaaatcgcga tagatctcct tcagcggcac
      901 gccgtcggac gagagatcga caccgctcga caaattcgtg ccgcgagttt cgacgagcag
      961 ctccgggcat tcctgccgga agtcgcgcca gaattggagc atcgcggtgc ggagctcgtt
     1021 ggctttggcc gacgtgtcga agcgcgcgcc gtcgaacaac gcgc
//
LOCUS       DASPLT010000750         1083 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_255113, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000750 DASPLT010000000
VERSION     DASPLT010000750.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1083)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1083)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1083
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_255113"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1035)
                     /locus_tag="VEA63_07825"
     CDS             complement(<1..1035)
                     /locus_tag="VEA63_07825"
                     /inference="COORDINATES: protein motif:HMM:NF012722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HYD83946.1"
                     /translation="MLGRLSPDPKGATWTHYSLSGLGVFGEINAMLVDDRGILWLGEN
                     DGVLRIDPEKLSPVSTPHPPTIHANVADGGQIEANDNSIEFDAGSVEYARRESVRFQT
                     RLSGRDEWSPATNTHQFTFAGLRDGDYAFAVRVVNDAGLTSTPASWSFTVLPPWYRTK
                     AAFALWTVLLFAACFGFVQWRSAYLRRRNLLLEELVRKKTDQLEKANAAKSEFLANMS
                     HEIRNPISGIVGLSLAMEETTLDPRQRNLTDSIRSCASLLATLVDDVLDFSKIEAGKI
                     DLRPAPFDLRKAVEQCAGMVTEEIRQKGGQLTLELHPDVPAQVLGDSARVQQIVLNYL
                     TNAVKFGAGKP"
BASE COUNT          193 a          310 c          373 g          207 t
ORIGIN      
        1 gggttttccc gcgccgaact tcaccgcgtt ggtgagatag ttcaggacaa tttgctgcac
       61 gcgcgcggag tcgccgagca cttgggccgg cacatccggg tggagctcga gcgtgagctg
      121 tccgcctttt tggcgaatct cctcggtcac cattccggcg cattgttcga cggctttgcg
      181 caaatcgaac ggcgcgggcc ggagatcgat tttgcccgct tcgatcttgg aaaaatcgag
      241 cacgtcgtcg acgagcgtgg cgagcaggct ggcgcaactg cggatcgaat cggtgaggtt
      301 gcgctggcgc ggatcgagcg tggtttcctc catcgccagc gagagcccga cgatgcccga
      361 gatcggattg cggatctcgt ggctcatgtt ggcgaggaac tccgatttgg cggcgttggc
      421 cttttcgagt tgatcggttt ttttgcgaac gagctcctcg agcaaaagat tgcggcggcg
      481 cagataggcc gagcgccatt gcacgaagcc gaagcaggcg gcgaaaagga ggacggtcca
      541 aagcgcgaag gcggctttcg tgcgatacca cggcggcaac acggtgaaac tccagctcgc
      601 gggcgtgctg gtcaggccgg cgtcgttgac cacgcgcacc gcgaacgcgt agtcgccatc
      661 gcgcaagccg gcgaaggtga actggtgcgt gttcgtcgcg ggcgaccatt catcgcggcc
      721 gctgagacgc gtctggaatc gcaccgattc gcggcgcgcg tattcgaccg agccggcgtc
      781 gaactcgatg gaattgtcgt tcgcttcgat ctgtccaccg tcggcgacgt tcgcatggat
      841 cgtcggcggg tgcggcgtcg aaacggggga gagtttctcc ggatcgatac ggagcacgcc
      901 gtcgttttcg ccgagccaga ggatgccgcg atcgtccacg agcatcgcgt tgatttcgcc
      961 gaaaacgccg agcccgctga gcgaatagtg agtccacgtg gcgcctttgg gatcgggcga
     1021 gagacgcccg agcacgggcg cgcgcgaacc atctttgaag gggcttttaa aggcggccca
     1081 gat
//
LOCUS       DASPLT010000751         1049 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_194316, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000751 DASPLT010000000
VERSION     DASPLT010000751.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1049)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1049)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1049
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_194316"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            70..>1049
                     /locus_tag="VEA63_07830"
     CDS             70..>1049
                     /locus_tag="VEA63_07830"
                     /inference="COORDINATES: protein motif:HMM:NF014919.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HYD83947.1"
                     /translation="MRQFYDPYAFLPTLGEQDLYLFNEGNEHRIYDKLGAHVRTLGDV
                     PGVAFAVWAPAAKQVSLVGNFNHWDGRYHQMRPLGSSGVWELFVPGLEEGELYKFAVR
                     DQRGHVRLKTDPYGTYFEGPPGNASVVYDVGKIKWNDGAWLEKRRANAGKLDQPMSIY
                     EVHLGSWRKNAEDANRKLSYREAAVQLADYVTEMGFTHVEFMPLAEHPFTGSWGYQVT
                     GFYAPTHRHGSPEDFAWMVDYLHQRGVGVIIDWVPAHFPRDSFALAEFDGSHLYEHAD
                     PRLGAHMDWGTLIFNYGRNEVRCFLVANALAWLDRYHIDGLRVDAVASML"
BASE COUNT          185 a          273 c          376 g          215 t
ORIGIN      
        1 gaagtgttcg tcgggaagcg gtccgaggtt tttggatacc agcttcgggt caagacgagt
       61 agcggcgaga tgcggcagtt ctatgatccg tatgcgtttc tgccgacgct cggggagcag
      121 gacttgtatc tcttcaacga aggcaacgag caccggattt acgacaagct cggcgcgcat
      181 gtgcggacgc tcggcgacgt gcccggcgtg gcgtttgcgg tgtgggcgcc ggcggcgaag
      241 caggtgtcgc tcgtgggcaa cttcaatcac tgggatgggc gttatcatca gatgcgtccg
      301 ctgggctcgt cgggcgtgtg ggaattgttc gtgccggggt tggaggaggg tgagctctac
      361 aaatttgccg tgcgcgatca gcgcgggcac gtgcggctga agacggaccc atacggcacg
      421 tatttcgaag gcccgcccgg aaacgcgtcg gtcgtgtatg acgtgggcaa gatcaagtgg
      481 aatgacggtg cgtggctgga gaaacgtcgc gcgaacgcgg ggaaactcga ccagcccatg
      541 tcgatctacg aagtgcacct cggctcgtgg cggaagaacg cggaggatgc gaatcgcaag
      601 ctgagttacc gcgaggcggc ggtgcaactg gcggactacg tgaccgagat gggcttcacg
      661 cacgtggagt tcatgccgtt ggcggagcat ccgttcacgg gctcgtgggg ttatcaagtg
      721 actggttttt atgcgccgac gcaccggcat gggtcgccgg aggactttgc gtggatggtc
      781 gattatctgc accagcgagg ggtcggcgtg atcatcgatt gggtgccggc acattttccg
      841 cgcgacagtt tcgcgctcgc ggaattcgac ggctcgcatc tctacgagca cgcggatccg
      901 cgccttggcg cgcacatgga ttgggggacg ctgatcttca actacggccg caacgaagtg
      961 cggtgtttcc tcgtggcgaa cgcgctcgcg tggctcgatc gctatcacat cgacggtttg
     1021 cgcgtggatg ccgtggcgtc gatgctcta
//
LOCUS       DASPLT010000752         1067 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_132573, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000752 DASPLT010000000
VERSION     DASPLT010000752.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1067)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1067)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1067
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_132573"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1044)
                     /locus_tag="VEA63_07835"
     CDS             complement(<1..1044)
                     /locus_tag="VEA63_07835"
                     /inference="COORDINATES: protein motif:HMM:NF012722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HYD83948.1"
                     /translation="MAVVATVLWFATAINSWLDGVFRFYPRATVARQAAFTVARRESR
                     TDSLEKAFVDVLKGWGQAEHAVLISGGKGGLKGDGIQLSEESVVVKTLREQRWVTPER
                     LARERPSPARNEIGAFLAEHQLGVLVIGEGRTLTVIVGVGAAASRRPFTYPQVTQLME
                     LASIMESAMERAHFSVKAQHAEQLATVGLLGASLAHEIRNPLVTIKTFVQLLPKHYQE
                     QNFREKFFTLIGDEVTRIDRLTEQLLDLASPRAYKAEAIELHPVLRTSLELVAAKATD
                     KRIQFLTDFQANPDRAFTDASAVKQVMLNLCFNALQAVEAKTDERWVKVCTRNVARGI
                     EMTVEDSGPGIAPE"
BASE COUNT          202 a          371 c          286 g          208 t
ORIGIN      
        1 ttccggcgcg atcccagggc cgctgtcctc gaccgtcatc tcgattccgc gcgcgacatt
       61 gcgcgtgcaa accttcaccc accgctcgtc ggttttggcc tccaccgctt gcagcgcgtt
      121 gaagcaaaga ttgagcatca cctgtttcac cgccgaggca tccgtgaacg cgcgatccgg
      181 attcgcctga aaatcggtga gaaactggat gcgcttgtcc gtcgctttcg ccgcgaccaa
      241 ttccaaactc gtgcgcagca ccggatgcaa ttcgatggct tcggccttgt aggcccgcgg
      301 tgaagccaga tcgagcaatt gctcagtcag ccggtcgatc cgcgtcacct cgtctccgat
      361 caaagtgaag aatttttccc ggaagttctg ttcctgataa tgtttcggca gcagttgcac
      421 gaaggtcttt atcgtcacaa gtgggttccg gatttcgtgc gcaaggctcg cacccagtaa
      481 tcccaccgtg gccaactgtt ccgcgtgctg cgcttttacc gaaaaatgcg cccgctccat
      541 cgcgctttcc atgatcgacg ccagctccat caactgcgtg acctgcgggt aggtgaacgg
      601 ccgccgcgaa gccgcggcgc ccacgcccac gatcacagtg agcgtgcgcc cttcgccgat
      661 cacgagcacg ccgagttgat gctcggcgag gaacgccccg atctcgttcc tcgccggcga
      721 cggacgttcg cgcgccaatc gctccggcgt cacccagcgc tgctctcgca aagttttaac
      781 gactacactc tcctcgctga gttgaatccc atcgcccttc aggccgccct tcccgccgga
      841 aatgagcacc gcgtgctccg cctgtcccca acctttcaat acatcgacga acgccttctc
      901 caagctgtcc gtccgcgatt cgcggcgcgc gacggtgaac gccgcctggc gcgcgacggt
      961 ggcgcgcggg tagaaacgaa agacaccatc gagccaggaa tttatcgccg tcgcaaacca
     1021 caacacggtt gccacgaccg ccacgaacgc cagccactgc ggcagga
//
LOCUS       DASPLT010000753         1420 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_132595, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000753 DASPLT010000000
VERSION     DASPLT010000753.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1420)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1420)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1420
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_132595"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            465..1097
                     /locus_tag="VEA63_07840"
     CDS             465..1097
                     /locus_tag="VEA63_07840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD83949.1"
                     /translation="MQTNDTTTPATVAAPQAGTAESAQALAQAATQAAQAKQAKAAER
                     RAKPSKPAASAKPAKPAKQAKQAAKPATAKAKAVKPADPAAIKARAERQAVITGHRQA
                     VAAIYSGASLTVHSSRAAPVNAYVERISAPVQRIGKSGPTTRDNSLLALLARNAKPNG
                     DFDPVALACDVGAISRLASAGFVTLRDGTPNLTKAGRERGNNEHRKANAS"
BASE COUNT          300 a          539 c          344 g          237 t
ORIGIN      
        1 gtatcgttta tcgataagga gaagaaggtg tatcgataaa agatgaacat tgatcgataa
       61 tcgatgtagt tgttgtgcgg ctgtcacact ggccagacaa aagtgcagtc gcctgacgat
      121 ttggattgac tgtagttttg cggtcacacg ctcagttgcc cggccctcca tatcgggaaa
      181 cgataaatcc acgctaagcc ccatttccgg ccctacagcg cccctctcct accccgccgc
      241 atgcgaccca gcggctacca tcgccgcatg ccctcagcgg ccacgtatcg gctaaacagc
      301 catattcgcc ccctcccctg ctaacgttat cagcggctcg cccctccccc catcgtcgcg
      361 gcgttacact ttttcgcatt cccctattgc aaccttcggc gcgttcgcgc atatctttcg
      421 tcggcggcgc acttctgccc cgccactgca aacggagcca acccatgcaa accaacgaca
      481 ccaccacccc cgccaccgtc gccgctcccc aagccgggac cgccgaaagc gcccaagccc
      541 tcgcccaagc ggccacgcaa gccgcgcaag cgaagcaagc gaaagccgcc gaacgccgcg
      601 ccaagccgtc caagcccgcc gcctcggcca agcccgccaa gcctgcgaag caagcgaagc
      661 aagcggccaa gcccgcgacg gccaaagcga aagccgtcaa gcccgccgac cccgccgcga
      721 tcaaagcccg cgccgaacgg caagccgtca tcaccggcca ccgccaagcc gtcgccgcga
      781 tctactcggg cgcgtccctc acggtccaca gcagccgggc ggctcccgtc aacgcctacg
      841 tcgagcgcat cagcgccccc gttcagcgca tcgggaaaag cggacccacc acccgcgata
      901 acagcctgct cgccctgctc gcgcgcaacg ccaagccgaa cggggatttc gacccggttg
      961 cgctcgcctg cgacgtcggc gcaatctctc gcctcgcctc ggccggtttc gtcaccctgc
     1021 gggacggaac cccgaacctc acgaaagccg ggcgggagcg cggcaacaac gagcaccgca
     1081 aagcgaacgc cagctaagcg agccctccga actcaagcgc cccggccaca agccggggcg
     1141 ttccctttct ccacacacgg gacttagccc tatgcttgct ctaacctttg ctttccttat
     1201 cggctcttcc gtaatactcg gttgcatcct agcaatcgcg gcgactatta cggaacacag
     1261 caacgcggaa cgtgcggcaa gctttattgc gtccgttaca ctattcagcg tcgcactatt
     1321 cacggcctat attgcgtcgt gcatccctaa cctttcccaa tactaataac gtccctaaaa
     1381 aaccagccga ttacttcggc accccctccc gctgtatgtt
//
LOCUS       DASPLT010000754         1513 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_255161, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000754 DASPLT010000000
VERSION     DASPLT010000754.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1513)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1513)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1513
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_255161"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..282
                     /locus_tag="VEA63_07845"
     CDS             <1..282
                     /locus_tag="VEA63_07845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD83950.1"
                     /translation="WQAVTGTQPGERASRFVDPVFTSTSSGNSHSQSDANGADQRGSN
                     PQHDRRLADDLAALRGAARTQSGAVAVAAQPSLARRAPLSTSSHLHTFA"
     gene            289..762
                     /locus_tag="VEA63_07850"
     CDS             289..762
                     /locus_tag="VEA63_07850"
                     /inference="COORDINATES: protein motif:HMM:NF015896.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook capping FlgD N-terminal
                     domain-containing protein"
                     /protein_id="HYD83951.1"
                     /translation="MSVSPIGQNYQTRAASDDTDATTTAPKKKTQLGQEDFLKLLAVQ
                     FKSQDPMKPIEDTAFIAQMAQFSALDQSSTLVQQMTELRSNQDIATANTYIGRHVTLD
                     AGDDEVVSGDVSGVDVSDGTPRLIVGDKTYPLSSVLLVEPGTTPPPSQPANAASA"
     gene            788..>1513
                     /locus_tag="VEA63_07855"
     CDS             788..>1513
                     /locus_tag="VEA63_07855"
                     /inference="COORDINATES: protein motif:HMM:TIGR03506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein"
                     /protein_id="HYD83952.1"
                     /translation="MALIGTLTSGISALKTFSKGLDVIGNNIANVNTTAYKSSSASFA
                     DTFSNTLRASATGTSAMQVGTGVQLAGITANYTQGSVSSTGNATDLAISGNGYFVVRN
                     AADGQLFATRAGNFRIDEQGYLVTSNGYRVQGATGGTASLPPATIGNIRLKTSAEIAA
                     EWATNNAVEIHDTEIARDAADGAATALNALVTANAGAATAADVLTSIQTARDAAVTDT
                     ATASAALALDPTNQSLQDALILAQ"
BASE COUNT          289 a          584 c          398 g          242 t
ORIGIN      
        1 tggcaggcgg tcaccggcac gcagcctggc gaacgcgcgt cgcgtttcgt cgatcctgtt
       61 ttcacttcga cgtcctcggg taactcgcac tcgcagtcgg acgcaaacgg cgcggatcaa
      121 cgcggctcca atccgcagca cgatcgccgc ctggccgacg atctcgcggc gcttcgcggc
      181 gcggcgcgca ctcaatccgg tgcagtcgct gtcgccgctc agccgtccct cgcccgccgc
      241 gcgccgctct ccacctccag ccacctccac acgtttgcct gatttaccat gagcgtctca
      301 cccatcggcc agaactacca aacccgggcg gcgtccgacg acacggacgc cacgaccacg
      361 gctcccaaga aaaagaccca gctcgggcag gaggattttc tgaagctcct cgcggttcaa
      421 ttcaagtcgc aggacccgat gaaacccatc gaagacacgg cgttcatcgc gcagatggcg
      481 cagttcagcg cgctcgatca atccagcacg ctcgtgcagc agatgaccga gctccgctcg
      541 aatcaggaca tcgccaccgc caacacctac atcggccgcc acgtcacgct cgacgctggt
      601 gatgacgagg tcgtgtccgg cgacgtttcc ggcgtcgacg tcagcgacgg cacgccgcgc
      661 ctcatcgtcg gcgacaagac ttatccactc tcctccgtgc tcctcgtgga gccgggcacc
      721 actcctcctc cgtcgcaacc ggccaacgcc gccagcgcct agtcctcaac ctccatactc
      781 aaccaccatg gccctcattg gcactctcac ttccggcatc agcgcgttga agactttcag
      841 caaaggtctc gacgtgatcg gtaacaacat cgcgaacgtc aacacgactg cctacaagag
      901 ctcctccgcg agctttgccg acacgttcag caataccttg cgcgcgtccg ccaccggcac
      961 gtccgcgatg caagtcggca ccggcgttca actcgcgggc atcaccgcca actacaccca
     1021 gggctcggtc tcgagcacgg gcaacgccac cgatctcgcg atctcgggca acggttactt
     1081 cgtcgttcgc aacgccgccg acggacagct cttcgccacc cgcgccggca atttccgcat
     1141 cgacgaacaa ggctatctcg tcacctcgaa cggttatcgc gttcaaggcg ccaccggcgg
     1201 caccgcgtcg ctcccgcctg cgaccatcgg caacattcgc ctcaaaacgt cggctgaaat
     1261 cgccgccgaa tgggcgacca acaacgcggt cgaaattcac gatacggaaa tcgcccgcga
     1321 tgccgccgac ggcgccgcca ccgcgttgaa cgcgctcgtc accgccaacg ccggcgctgc
     1381 cacggccgcc gacgtgctga cgtcgattca gaccgcccgc gatgccgccg tcactgacac
     1441 cgccacggcg tccgctgcgc tcgcgctcga tcccacgaat caatccctgc aagacgcgtt
     1501 gatcctcgcc cag
//
LOCUS       DASPLT010000755         1312 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_296164, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000755 DASPLT010000000
VERSION     DASPLT010000755.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1312)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1312)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1312
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_296164"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(60..>1312)
                     /locus_tag="VEA63_07860"
     CDS             complement(60..>1312)
                     /locus_tag="VEA63_07860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD83953.1"
                     /translation="GPQSGLYQLRVNGEEIHVSDESRFDFHTAAFTLSRPVTVEVTLP
                     VGANTPVVRPLRHKLYPTFHDGKATFTLSEPLNLVIQAEGLPPLALFATPAETDVPRP
                     DDPKVLYFGPGIHEPGVIQPRSGQTIYLADGALVKGRIEATHVTGVRVRGRGVLDASA
                     YSIRQDKTPGILFEKCADIRVEGIGLRGGSWWQVLFLLTEDAEVAHMNILGVSVNTDG
                     IDIDGVRNFVARNCFIRCEDDGFGWHAVDAKRNGEPPTENCLAEDCVIWNTRYGNGLR
                     VGASMETQLFRNITFRNIDVLEHAGAAIRSDHSDWALCENIRFENFIDESAGRVVEIV
                     IARTRYSNDNGYRDERGRFRGLHFKNVHSAGGAIVLKGHDAEHAIDDVTFENCTIARR
                     PLQSVGDIEVNEFVRNISFDAPWR"
BASE COUNT          247 a          433 c          378 g          254 t
ORIGIN      
        1 gcctgccagc cgccacgcag cccccgcggc ccgccgcacg tttcgacgac cgcttcacgt
       61 tagcgccacg gcgcgtcaaa agagatgttc ctgacgaact cgttcacctc gatgtcgccg
      121 acgctttgca acggtcttcg agcgatggtg cagttttcga aagtgacatc gtcgatcgca
      181 tgctccgcat cgtggccttt aagaacgatg gcgcctcccg ccgagtgcac gttcttgaaa
      241 tgcaggccgc ggaagcgccc gcgctcatcg cggtaaccgt tgtcgttgct atagcgggtt
      301 cgcgcgatga cgatctccac tacccggccg gccgactcgt cgatgaagtt ttcaaagcgg
      361 atgttttcgc agagcgccca atcggagtga tcgctccgga tcgcggcgcc cgcgtgctcc
      421 aggacgtcga tgttgcgaaa cgtgatgttc cggaagagct gggtttccat cgacgcaccg
      481 acgcgcagcc catttccgta gcgcgtattc cagatcacgc agtcctccgc caagcagttc
      541 tccgtgggcg gctcgccgtt tcgcttcgcg tcgacggcat gccagccgaa gccgtcgtcc
      601 tcgcaacgaa tgaaacaatt ccgcgccacg aagtttctca cgccgtcgat gtcgatgcca
      661 tcggtgttca ccgacacccc gaggatgttc atgtgcgcaa cctccgcgtc ctccgtgagc
      721 agaaacaaaa cctgccacca acttccgccg cgaagcccga tgccctccac gcggatgtcg
      781 gcgcacttct cgaaaagaat tcccggcgtt ttgtcctgcc gaatcgaata cgcgctggcg
      841 tcgagcacgc cccggccgcg cacgcggacg cctgttacat gcgtcgcttc aatccggcct
      901 ttgaccaacg ctccatcggc caaataaatc gtctggccgc tgcggggttg gatgactccg
      961 ggctcatgga tacccgggcc gaaatacaac accttcggat cgtcgggccg cggaacgtcc
     1021 gtttccgcgg gagttgcgaa caaggccagc ggcgggagcc cctcggcctg aatcaccagg
     1081 ttcagcggct cggacaacgt gaatgtcgct ttgccgtcat gaaacgtcgg atacagttta
     1141 tgccgcaaag gtcggacgac gggcgtgttc gcgccgaccg gcagggtaac ttcaaccgtc
     1201 accgggcgcg acagggtgaa cgccgcggtg tgaaaatcaa aacggctttc atcgctgacg
     1261 tggatctctt ctccattaac ccggagctga tagagcccgc tctgcggccc gg
//
LOCUS       DASPLT010000756         1098 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_69407, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000756 DASPLT010000000
VERSION     DASPLT010000756.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1098)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1098)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1098
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_69407"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..414
                     /locus_tag="VEA63_07865"
     CDS             <1..414
                     /locus_tag="VEA63_07865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD83954.1"
                     /translation="SHLTKNEMTVSRPLGTDPTSTRNFLVLNGRPLDLAPDDTLRVYQ
                     RYEFLDGTLKGASVGLGVRYQSDFWPQASSVNWGIVFPASAIVDLNLGYKTKIQNRNV
                     SFLFGVNNVLDSLSIEGQRVFGAPREFYLSTRVSF"
     gene            708..>1098
                     /locus_tag="VEA63_07870"
     CDS             708..>1098
                     /locus_tag="VEA63_07870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009510848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HYD83955.1"
                     /translation="MLLFGAAVLNYVDRQTLSALAPTIQRDLAMDDRDYANVVNLFLV
                     AYTLAYLVAGRLTDALGTRAGMAVFVAWWSLSNALTAAAVGVRSLGVFRFSLGLGEAG
                     VWPAASKAVSEWFPSRERALAIGVYTMG"
BASE COUNT          188 a          352 c          312 g          246 t
ORIGIN      
        1 tcgcacctga cgaaaaacga gatgaccgtt tcgcgtccgc tcggcaccga tccgacgagc
       61 acgcggaatt tcctcgtgct caacggccgt ccgctcgacc tcgctccgga tgacacgctg
      121 cgtgtttacc agcgctacga attcctcgat ggcacgttga aaggcgcatc cgtgggcctc
      181 ggtgttcgct accaaagcga tttctggccg caggcctcga gtgtgaattg gggcatcgtt
      241 ttcccggcct cggcgatcgt ggacctcaat ctcggctaca aaaccaagat ccagaatcgc
      301 aacgtgagct tcctcttcgg tgtgaacaac gtcctcgatt cgctctccat cgaaggccag
      361 cgcgtgttcg gagcgccgcg cgagttttat ctctccacgc gcgtttcttt ctaaacgcag
      421 ttcaccgaag tgacggcctc cgcagcgaaa gcccatccga caacacggca gctatcgttt
      481 cgaaattggc ggagccgatc tccgagcggt ccgcccggag ggtggaccct ttcccttcgg
      541 ctacgacaaa atccaagtca cacggctccc gactccccgc gaagtttggg agccgttttt
      601 attccgctct ctagattctt caacgatcgt ttcctcccgc gctctcgatt aagcagacgc
      661 gtgatcgccg aatgagaaaa atcccacacc tgcgttggtg gatcgccgtg ctccttttcg
      721 gtgcggcggt gctgaactac gtagatcgcc agacgctctc ggcgctcgcc cctacgattc
      781 agcgcgatct cgcgatggac gaccgcgatt acgcgaacgt cgtcaatctc ttcctcgtcg
      841 cctacacgct cgcgtatctc gtggcaggac ggctgaccga tgcgcttggc acgcgcgctg
      901 gcatggccgt gtttgttgct tggtggtcac tcagcaacgc gttaaccgcg gcagccgtgg
      961 gcgttcgctc cttgggggtt ttccgttttt cgcttgggct cggcgaagcc ggcgtgtggc
     1021 cggcggcctc gaaagccgtc tcggagtggt ttccctcgcg cgaacgagcg ctcgccattg
     1081 gcgtgtatac gatgggcg
//
LOCUS       DASPLT010000757         1126 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_173572, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000757 DASPLT010000000
VERSION     DASPLT010000757.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1126)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1126)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1126
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_173572"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1103)
                     /locus_tag="VEA63_07875"
     CDS             complement(<1..1103)
                     /locus_tag="VEA63_07875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HYD83956.1"
                     /translation="MTGNLVMRPTGAFEGLCGPDCTEIAPLLSKAEHSNTAVIFGDRF
                     LFKLFRRPEAGMNPDVEIMGYLTAKNFPNVPTLGGAMEYRTTGSVRSIGILTQFIAGA
                     NDGWDYTLDALGRYFDRALLYVAEGRPCSPPEMALLDLAEQEPTPDVVEAIGTYLESA
                     RLLGARTAELHLALGSGGGPDFAPEPFSPHYQRSLYQSMRNQVMENFELLKRQVRGLP
                     EGLQTDAQAILAAQDAVLERYKLLYTDRLDAMRIRVHGDYHLGQVLSTGTDFVILDFE
                     GEPARALSARRLKRSPVSDVAGMIRSFNYAVHTALQTVADRGLLTPENRDGAVAWATF
                     WRRWISATFVGAYRRTMGETRLLPRDRRQFEILL"
BASE COUNT          219 a          435 c          280 g          192 t
ORIGIN      
        1 agcagaatct cgaactgccg ccgatcgcgc ggcagcaaac gcgtttcgcc catcgttcgc
       61 cgataagcgc ccacgaacgt cgccgagatc caccgccgcc agaacgtcgc ccacgccacc
      121 gcgccgtcgc ggttctccgg cgtcagcagc ccccgatccg ccaccgtttg cagcgccgtg
      181 tgcaccgcat agttaaacga ccgaatcatc cccgcgacgt cgctcaccgg cgaacgcttc
      241 aaccgccgcg cgctcaacgc tcgcgccggc tcgccttcga aatccaaaat cacgaaatcc
      301 gtgcccgtcg aaagcacctg ccccaggtga taatccccgt gcacccgaat tcgcatcgcg
      361 tcgagtcggt cggtgtagag cagcttgtaa cgttccagca ccgcgtcctg cgccgcgaga
      421 atcgcctgcg catctgtctg caaaccttcc ggcaacccgc gcacctgccg tttcaacagc
      481 tcgaaattct ccatcacctg gttgcgcatc gattgataca atgaccgttg gtaatgcggc
      541 gaaaacggct ccggtgcgaa gtccggtccg cctccactcc ccagcgccag atgcagctcc
      601 gccgtccgcg cgcccagcaa tcgcgccgac tcgagatacg tgccgatcgc ctccactacg
      661 tccggcgtcg gctcctgctc cgccaaatcg agcagcgcca tttccggcgg cgaacacggc
      721 cgcccctccg ccacatacag cagcgcgcga tcgaaatacc gcccgagcgc atcgagcgtg
      781 taatcccatc catcgttcgc accggcgatg aactgcgtca gaatgccgat gctccgcacg
      841 cttcccgtcg tgcgatactc catcgcacca cccagcgtcg gcacattcgg aaaatttttc
      901 gccgtgagat agcccatgat ctccacgtcc ggattcatcc ccgcctccgg ccgccggaaa
      961 agtttgaaca aaaaccgatc gccaaaaatc accgccgtgt tgctgtgctc cgccttcgac
     1021 agcagcggcg caatctccgt gcaatccggt ccgcacagtc cttcgaacgc acccgtcggc
     1081 cgcatcacca gattcccggt cacgccgcgc agcgtcgctc cttgct
//
LOCUS       DASPLT010000758         1110 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_33067, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000758 DASPLT010000000
VERSION     DASPLT010000758.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1110)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1110)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1110
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_33067"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..673)
                     /locus_tag="VEA63_07880"
     CDS             complement(<1..673)
                     /locus_tag="VEA63_07880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012377023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HYD83957.1"
                     /translation="MPLKLSRRQYAEMFGPTVGDRVRLADTELFVQVERDLIAEGGGY
                     GNEVKFGGGKVIRDGMGQSSTATDAESLDLVITNALILDPVLGVIKADIGIKHGVIVG
                     IGHAGNPGIQSGLGSVYVDPRAKKKNPMIVGACTEVIAGEGCIVTAGGIDSHIHFICP
                     QQIDEAISSGITTMFGGGTGPAHGTLATTCTPGAWNLHRMLEAAEAYPMNLGFLGKGN
                     CSTPQP"
     gene            complement(784..1095)
                     /locus_tag="VEA63_07885"
     CDS             complement(784..1095)
                     /locus_tag="VEA63_07885"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007360046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0035550 - urease complex [Evidence IEA];
                     GO_process: GO:0043419 - urea catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit beta"
                     /protein_id="HYD83958.1"
                     /translation="MIPGEIIVAKDAAPLAANLELETKVLAVANTGDRPIQVGSHYHF
                     FETNEALRFDRAAARGFRLNIPAGTAVRFEAGDTKEVELVALAGAREVYGLNAKVNGK
                     L"
BASE COUNT          219 a          373 c          306 g          212 t
ORIGIN      
        1 acggctgcgg cgtggaacag ttgcccttgc caagaaaccc gaggttcatc ggatacgcct
       61 cagccgcttc gagcatgcga tgcaaattcc acgcgcccgg cgtgcaggtc gtcgccagcg
      121 tgccgtgcgc cggccccgtg ccgccaccaa acatcgtcgt gatgcccgag ctgatcgctt
      181 cgtcgatctg ctgcggacaa atgaaatgga tatgactatc gatcccgccc gcggtgacga
      241 tgcagccctc gcctgcgatc acctccgtgc acgcgccgac aatcatcggg ttcttcttct
      301 tcgcgcgcgg atcgacgtaa accgatccca gcccgctctg aatccccgga ttgcccgcgt
      361 ggccgatgcc gacgatcacg ccgtgcttga tgccgatgtc cgctttgatg acgccgagca
      421 ccggatcgag aatcagcgca ttcgtgatca ccaaatcgag cgactccgca tccgtcgccg
      481 tcgacgattg acccatgcca tcgcgaatga ctttgccgcc gccgaacttc acttcgttgc
      541 cgtagccgcc gccctcggcg atgagatcgc gttcaacttg aacgaacaac tccgtgtcgg
      601 cgagacgcac gcgatcgccc acagtcggtc cgaacatctc agcgtattgg cggcgggaaa
      661 gtttgagggg catggtcagg aaggtttgaa accacgaatg gacacgaatc gacacgaact
      721 aaaaccgtgt ccgtccggtc cggtcattcg tgccagttcg tgtccattcg tggttaaaac
      781 gactcagagc ttgccgttca ccttcgcgtt gaggccgtag acctcgcggg caccggcgag
      841 cgccaccaac tcgacttcct tcgtgtcgcc cgcctcgaac cgcaccgcgg tgccggcagg
      901 aatgttgaga cgaaaaccgc gcgccgccgc acgatcgaaa cgcagcgcct cgttcgtctc
      961 gaaaaaatga taatgcgaac cgacctgaat cggccgatcg cccgtattcg ccacggccag
     1021 caccttcgtc tcgagctcga gattcgccgc gagcggcgcc gcatccttcg cgacgatgat
     1081 ttccccagga atcatttcag cctttcaggt
//
LOCUS       DASPLT010000759         1170 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_194382, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000759 DASPLT010000000
VERSION     DASPLT010000759.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1170)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1170)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1170
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_194382"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            50..727
                     /locus_tag="VEA63_07890"
     CDS             50..727
                     /locus_tag="VEA63_07890"
                     /inference="COORDINATES: protein motif:HMM:NF018604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family protein"
                     /protein_id="HYD83959.1"
                     /translation="MFTKQVPSWIPLGGLALTSAAGCINAVGFLSLDHHAISHMSGNV
                     TVLGLELGRADYAGAMRTLTVLLCFFFGCLLSGLIIRESTLRLGRRYGVALACESTLL
                     VFATWYLRHGQHLGVYLAAMACGLQNAMATGYSGAVVRTTHMTGIVTDLGIAVGLWAR
                     RQPVDWRRMRLYLVLLLGFFVGSGLGAVGFAHFHYDTLLFPAALTGFGGIVYGIYKHR
                     TRRAGPH"
     gene            complement(832..>1170)
                     /locus_tag="VEA63_07895"
     CDS             complement(832..>1170)
                     /locus_tag="VEA63_07895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD83960.1"
                     /translation="RRGYRLDTTKIVGARTARKIRETRGQLGYEWAHLIRKLKVRAPE
                     LARRLRDVVSPEPHPLFRVIEGEVCEWEKQPVATLKPVRLRRTDGADPKRRQALRKSC
                     KAGNTERTER"
BASE COUNT          164 a          432 c          309 g          265 t
ORIGIN      
        1 gcccccggct gatcgcccgc cactccttcg cgcgcctccc ttcgccgcca tgttcacgaa
       61 gcaggtcccc tcttggatac cattgggcgg cctcgcgctc accagcgctg cgggctgcat
      121 caacgcagtc ggtttcctga gcctcgatca tcacgcgatc tcgcacatgt cgggcaacgt
      181 caccgtgctc ggcctcgaac tcggccgcgc cgattatgcg ggcgcgatgc gcaccttaac
      241 ggtcctcctt tgtttctttt tcggctgcct gctcagcggc ttgatcattc gcgaaagcac
      301 gctccgactt ggccgccgct acggcgtcgc gctcgcctgc gaatccacgc tgctcgtgtt
      361 cgccacgtgg tatctgcgcc atggccagca tctcggcgtt tatctcgccg cgatggcctg
      421 cggcctccaa aacgcgatgg ccaccggcta cagcggcgcc gtcgttcgga cgactcacat
      481 gactggcatc gtcaccgacc tcggcattgc ggtcggtctg tgggcccgcc ggcaacccgt
      541 cgattggcgc cgcatgcgtt tgtatctcgt gctgctgttg ggttttttcg tcggcagcgg
      601 cctgggcgcc gtcggcttcg ctcattttca ttacgacacc ttactcttcc ccgccgcgct
      661 caccggcttt ggcggcatcg tctacggtat ctataaacac cgcacccgcc gcgccggacc
      721 tcactgaggc tttcaagcct ccaactgcgc cgtcagtttt aatagcaaat cgcaccgcac
      781 cggcctgcct gcagtctatt ttttctggga tcttgctccc cgacctttgt gtcatcgctc
      841 cgtgcgctct gtgttccctg ccttacagct tttccggagc gcctgccgtc gttttggatc
      901 cgcaccgtcg gtgcgccgca gccgcacggg cttcagggtc gccaccggtt gtttctccca
      961 ttcacaaacc tcgccctcga tcacacgaaa cagcggatgc ggttccggcg acaccacatc
     1021 gcgcaaccgc cgcgccagct ccggtgcgcg aactttcaac ttccgaatca aatgcgccca
     1081 ctcgtagccg agttgcccgc gcgtctcgcg aattttccgc gccgtgcgcg cgcccacgat
     1141 tttcgtcgta tccaaacgat acccgcgccg
//
LOCUS       DASPLT010000760         1090 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_152583, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000760 DASPLT010000000
VERSION     DASPLT010000760.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1090)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1090)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1090
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_152583"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..692
                     /locus_tag="VEA63_07900"
     CDS             <1..692
                     /locus_tag="VEA63_07900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HYD83961.1"
                     /translation="AAGSGDPALHGGVGDCVDCNRCVQVCPTGIDIRHGLQMECIGCT
                     ACIDACDEVMTRLQRPKGLIRYDSQAAFSGGRTHWLRARTVIYGVLLLIGASVATWAL
                     TTVKPASFGVTRMTGAPYIVDEAAVRNQFLVRIVNKRNEPARFDLSLVEVPSALRQLG
                     FDTTVEVGPLGELVQPLVLQQPRESYTGPFHFAVRVEDVAGEMRLEREVEFLGPEARL
                     LREEEEAKRER"
     gene            complement(738..>1090)
                     /locus_tag="VEA63_07905"
     CDS             complement(738..>1090)
                     /locus_tag="VEA63_07905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD83962.1"
                     /translation="RSAHACTVQARDQTRDERGSRRVDGSEFHSEISKKCLRTRKNRR
                     ASLALLLRLLRLFAANHLPAPPVASGTSCTLCCDAIVNHAVHAPICIRKAATNHSHSR
                     DFPVAAGVPACLPV"
BASE COUNT          186 a          285 c          415 g          204 t
ORIGIN      
        1 cggcggcggg gtcgggagac cccgccctac atgggggcgt cggcgattgc gtggattgca
       61 atcgctgcgt gcaggtttgt ccgacgggga tcgatattcg tcatgggctg cagatggagt
      121 gcatcggttg cacggcgtgc atcgatgcgt gcgatgaagt gatgacgcga ttgcagcggc
      181 cgaagggact gatccgttac gattcgcaag cggcgttcag cgggggacgc acgcactggc
      241 tgcgggcgcg cacggtgatc tatggcgtgt tgttgctgat cggcgcgagc gtggcgacgt
      301 gggcgttgac gacggtgaag ccggcgagct tcggggtgac gcggatgacg ggcgcgccct
      361 acatcgtcga cgaggcggcg gtgcgaaatc agttcctcgt gcgcatcgtg aacaagcgca
      421 acgagccggc acggttcgat ctgtcgctcg tcgaagtgcc ctcggcgttg cgtcagctgg
      481 gtttcgacac gacggtcgaa gtggggccgc tcggcgaact cgtgcaaccg ctcgtgttgc
      541 agcagccgcg cgagagctac acggggccgt ttcatttcgc ggttcgcgtc gaggatgtgg
      601 ccggggaaat gcggctggag cgtgaggtgg agtttcttgg acccgaggcg cgtttgctgc
      661 gcgaggaaga ggaggcgaaa cgtgaacggt gattatcgca gcggcgggcc tccgtgcctg
      721 ccagcggatg acgcggctca aaccggcagg caggcaggga cgcctgccgc tacggggaag
      781 tcgcgactgt ggctgtggtt tgtcgccgct tttctgatac aaatcggcgc gtggaccgcg
      841 tggttcacga tcgcgtcgca gcacaaggtg caggaagtgc cgctggcgac gggaggagcg
      901 ggcaaatgat tggccgcaaa aaggcgcaaa aggcgcaaaa gaagagcaag ggaggcgcgg
      961 cggtttttgc gcgttctgag gcattttttg gagatctctg aatggaactc gctgccgtca
     1021 actcgcctgc tgccgcgttc atcgcgggtc tggtcacgag cctgcactgt gcaggcatgt
     1081 gcggaccgct
//
LOCUS       DASPLT010000761         2297 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_194401, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000761 DASPLT010000000
VERSION     DASPLT010000761.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 2297)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2297)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2297
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_194401"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..706)
                     /locus_tag="VEA63_07910"
     CDS             complement(<1..706)
                     /locus_tag="VEA63_07910"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HYD83963.1"
                     /translation="MKLPSRFPLLCAAPVLLIALASATPHARAQTPPDEVVRLDRYDV
                     VGVAPEDSVNPLTRPLEGVFGDLRGILETPRAVSTITQALLRERGIEGVREFVAFAPG
                     SYAPASYGKATIPNIRGDLAETFLNGQRLSYNNFGTMPSFNNVEAVDVVRGPGSALYG
                     SGFFTGGYVNYVTKQPKFTRETTLTARIGTWVPGGGSWLNGSWQIDTTAPAADGKSAW
                     RVSYEGKEDDTFFKRHG"
     regulatory      complement(772..885)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            1188..>2297
                     /locus_tag="VEA63_07915"
     CDS             1188..>2297
                     /locus_tag="VEA63_07915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD83964.1"
                     /translation="MLSRIASIALAGLLLLNVAQTQAHAQSLFKSDGSLNLSLYGLLA
                     LSYDKSTMETRLGPAFLDLSPSVKDDRPTLFRPTSKAYPTGYVRVNPFELGTKPAKDG
                     DYWSEAGQVGYVPDTTSNPGIDRIQLYAYYDKVFAISPRLDWSNKTPSPDLQAREPYY
                     ASIFGELPKYPIAMVRNYGMQCNEALVLYRDGNLAVVGTQTSRSNHERPFPGFSFPVE
                     KVPTAIAITTANEFALVTVWDTKLKKGQLAVIALEGKYLPFHTWPYMGLPNQGSWSAF
                     KLLGYVDLPMKTPSAVAAASNGFWNGPSQTAGRVLSQINLAKDGDRKNVYSGSWAGVV
                     AKNGYAIVASQHEDMVALVDLTPLFSYMRESYLSSA"
BASE COUNT          443 a          760 c          657 g          437 t
ORIGIN      
        1 caccgtggcg cttgaagaac gtgtcgtcct ccttgccttc gtaactcacg cgccacgcgg
       61 atttgccgtc cgccgccgga gccgtcgtgt cgatttgcca cgagccgttg agccatgagc
      121 cgccgccggg cacccacgtg ccgatgcgcg ccgtcagcgt cgtctcccgc gtgaacttcg
      181 gctgcttcgt cacatagttc acatacccgc cggtgaaaaa accgctgcca tatagcgccg
      241 aaccagggcc gcgcacgacg tcgaccgctt cgacgttgtt gaacgacggc atcgtgccga
      301 agttgttata actgagccgc tggccgttga gaaaagtctc cgccagatcg ccgcgaatat
      361 tcggaatcgt cgccttgcca taactcgccg gcgcgtagct gcctggcgca aaagcgacga
      421 actcgcggac gccttcgatg ccgcgctcgc gtaacagcgc ctgcgtgatc gtcgacaccg
      481 cgcgcggcgt ttcgaggatg ccgcgcagat cgccgaatac gccctcgagc ggacgcgtca
      541 gcggattcac ggaatcctcc ggcgcgacgc ccacgacgtc gtagcggtcg agccgaacga
      601 cttcgtcggg gggagtttgt gcgcgcgcgt gcggcgtggc gctggcgagc gcgatgagca
      661 gcaccggagc ggcgcagagc agtgggaatc gtgacgggag cttcatggtt gatgatgccc
      721 gacccaaagc ggactcccgg gagaaggggc gccgcgcgcc ccgaggcgcg atgaacggcg
      781 cctgctgttt ccttcgtcgg catgatccgt ttcaggttcg aagggtcgag cggccgagcg
      841 tgccgcaatc tcagtcctcc gcggaggaca cccctacagg cgacgtcata aagtttttgc
      901 ccgcggtaaa tcgcaaggaa cttctttcca cgatgtttgt agaacccggc tcctgctgca
      961 actgtcgggg cattctggta gccttctctg ggaaattctt tgcgataaga tccccgacgc
     1021 agctcctgag cgcctcgcct cggcgtatcc ggttttaccc tacggtgcgg caggatacct
     1081 taccggtttt taactaggta gaggcttccc ctaaagcctc ggggtgttct catagaaaca
     1141 ggtccggcag ccatgcagct aaatcccaaa gatcaatcct ccaaagaatg ttgtcacgca
     1201 ttgcgagcat agccctagcc ggccttctcc tccttaacgt cgcccagacc caagcccacg
     1261 cccaatcgct cttcaagtca gacggcagtt tgaacctgtc gctctacggc ttgctcgcgt
     1321 tgagctacga caaatcgacc atggagacgc gcctcgggcc cgctttcctg gacctcagcc
     1381 ccagcgtgaa ggacgaccgt ccgacgctct tccggcccac gtcgaaggca tatcccacgg
     1441 gctacgttcg cgtgaatccg ttcgagctcg gcaccaagcc cgcgaaggat ggcgactatt
     1501 ggagcgaagc cggccaagtc ggttacgttc ctgacaccac gagcaatccc ggcatcgatc
     1561 gcatccagct ttacgcctac tacgacaagg tcttcgcgat cagtccgcgg ctcgattggt
     1621 cgaataaaac accgagcccc gatctccagg cgcgcgaacc gtattacgcc tcgatcttcg
     1681 gcgagctccc gaaatatccg atcgcgatgg tgcgcaacta cggcatgcag tgcaacgaag
     1741 cgctcgtgct ttaccgcgat ggcaatctcg ccgtcgtcgg cacgcagacc agccgcagca
     1801 accacgaacg tccttttccc ggtttctcgt ttcccgtgga gaaagtgccg accgcgatcg
     1861 ccatcacgac ggccaacgag ttcgcgctcg tcaccgtctg ggataccaag ctgaaaaaag
     1921 gccagctcgc cgtcatcgcg ctcgagggga aataccttcc cttccacacg tggccctaca
     1981 tgggcttgcc gaatcaaggc agttggtccg ccttcaagct gctcggctat gtcgacttgc
     2041 cgatgaaaac gccgtcggcc gtcgccgccg ccagcaacgg tttctggaac ggcccgtccc
     2101 aaaccgcagg ccgcgtgctc agtcagatca atctcgcgaa ggacggtgat cgcaaaaacg
     2161 tctacagcgg ctcctgggcc ggcgtcgtcg cgaagaacgg ttacgcaatc gtcgcttcgc
     2221 agcacgagga catggtcgcc ctcgtcgacc tcacgccgct gttctcttac atgcgcgaga
     2281 gctatcttag ctccgcg
//
LOCUS       DASPLT010000762         1509 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_152604, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000762 DASPLT010000000
VERSION     DASPLT010000762.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1509)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1509)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1509
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_152604"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1222
                     /gene="ilvA"
                     /locus_tag="VEA63_07920"
     CDS             <1..1222
                     /gene="ilvA"
                     /locus_tag="VEA63_07920"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007362375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - L-threonine ammonia-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HYD83965.1"
                     /translation="MPVTTPEVKVNAVKARGAEVVLFGDSFDEAQAHARELEQRDGLT
                     FVHPFDDPDVIAGQGTIGLEILHQHQGPLHAIFIAIGGGGLAAGVAACLKRVRPEVKI
                     IGVEPVDADSMSRSLKAGHRVKLEHAGLFADGVAVREVGHETFRLCREYLDEVILVDT
                     DDICAAIKDVFEDTRAILEPAGAVAIAGAKAYVERERIAGQTLIAIACGANLNFDRLR
                     FIAERANIGEHREAVLAVTIPETPGSLRKFCQLLGRRSVTEFVYRIADTKNAHVFLGV
                     QANGRGEGERLAASFTEHGFYALDLTHDEMAKLHLRHLVGGRSPLAQDEVLYRFEFPE
                     RPGALLQFLESMSPNWNISLFHYRNHGADYGRVLIGLQVPAGDKPALQNFLETVGYLY
                     WDESDNPAYQLFLR"
     gene            complement(1284..>1509)
                     /locus_tag="VEA63_07925"
     CDS             complement(1284..>1509)
                     /locus_tag="VEA63_07925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD83966.1"
                     /translation="TPSYIICEAVHADVPWRGDVVKEGFTVETKGRIVRRNRKPGLGI
                     ELDEVEIRKHPFEQEVLQRTFYRDGSVGDW"
BASE COUNT          274 a          591 c          381 g          263 t
ORIGIN      
        1 gatgcccgtc accacacccg aagtgaaggt taacgccgtc aaagcccgcg gcgccgaggt
       61 cgtgttattc ggcgactcct tcgacgaagc gcaggcgcac gcccgagagc tcgagcagag
      121 agacggcctc accttcgtcc acccgttcga cgatcccgat gtgatcgccg gccagggcac
      181 catcggcctc gaaattctgc accaacatca aggcccgctc cacgcgatct tcatcgccat
      241 cggcggcggc ggtctcgctg cgggcgtcgc cgcctgcctc aaacgcgtgc gccccgaagt
      301 gaagatcatc ggcgtcgagc ccgtcgacgc cgactcgatg tcgcgttcgc tcaaagccgg
      361 ccaccgcgtg aagctcgaac acgccggtct ctttgccgac ggtgtcgccg tccgcgaagt
      421 cggccacgaa accttccgcc tttgccgcga atacctcgac gaggtcattc tcgtcgacac
      481 cgacgacatt tgcgccgcga tcaaagacgt gttcgaagac acccgcgcca tcctcgaacc
      541 cgccggcgcc gtcgcgatcg ccggcgccaa agcctacgtc gagcgcgagc gcatcgccgg
      601 ccagacgctc atcgcgatcg cctgtggcgc gaatctcaac ttcgaccgtc tccgcttcat
      661 cgccgagcgc gccaacatcg gcgaacaccg cgaggccgtg ctcgccgtca cgattcccga
      721 aacacccggc agtctccgca aattctgcca actcctcggc cgccgcagcg tcaccgagtt
      781 cgtttatcgc atcgccgaca cgaaaaacgc ccacgtcttt ctcggcgtcc aagccaacgg
      841 tcgcggtgaa ggcgaacgtc tcgccgcatc cttcaccgaa cacggcttct acgcgctcga
      901 tctcacccac gacgaaatgg ccaagcttca ccttcgacac ctcgtcggcg gacgctcccc
      961 gctcgcgcag gacgaagtcc tctaccgctt cgaattcccg gagcgccccg gcgcgctgct
     1021 acagttcctc gaatcgatgt cgcccaactg gaacatcagc ctcttccact accgcaatca
     1081 cggcgccgac tacggccgcg tcctcatcgg cctccaagtc cccgccggcg acaaacccgc
     1141 cctgcagaat ttcctcgaaa cggtcggcta cctctactgg gacgaatccg acaaccccgc
     1201 ctaccaactc ttcctccgct agctagcgta gcggcaggcc tccgttcctg ccagctcgtc
     1261 aggttgcgcg ctcctcgtct cgctcaccaa tctcccacgc tgccgtcgcg ataaaacgtc
     1321 cgctgcagca cctcctgctc gaacgggtgc ttccgaatct ccacctcatc cagttcgatc
     1381 ccgagccccg gcttccgatt tcgccgcacg atccgcccct tcgtctccac cgtgaaacct
     1441 tccttcacga catccccgcg ccacggcacg tctgcgtgca ccgcttcgca gataatgtag
     1501 ctcggtgtc
//
LOCUS       DASPLT010000763         1507 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_48935, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000763 DASPLT010000000
VERSION     DASPLT010000763.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1507)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1507)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1507
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_48935"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1507)
                     /locus_tag="VEA63_07930"
     CDS             complement(<1..>1507)
                     /locus_tag="VEA63_07930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD83967.1"
                     /translation="LVQAKFSRRSGLYIVVNPDDTNRTGRMPDGTAVIMRARSYRAFL
                     NTVNADGTHTIQEVVLPGQPSYGDLNAHVNRMPNAYRTNSSIGYNQVLRIPQGGGVDG
                     ADTGYASPTVPTAFPTYADSYFYDMRRGDGSNGYPFNRPSNNFIPRPIAKIDFDLTRF
                     KMAVERTLLTATSSTVYYPSRPTSATWDSSIFNPSAATTARGLGLGASFDLFPPVEVT
                     VTGYQRANTAAEQPYSARYVIAESTNGTSFTDVATYSSNTASLAHFANASSTHVRVRQ
                     YLHDQTIGSSYLIDEQILPILVADGSSTVGAALSNDYHLVPTRTDGSSPVFNDAYTTM
                     HVYVGGVEDTANWTFSATTTNVSGNFGSGATANRYTVTGLSANTGDVTITATKGGTTI
                     VRTFRLEKQGSKNTAIETLERQGRWLTASALSNPDPFRIFFAPGNPNDPLIDSDPETF
                     AVGAGDLVSTSGPSPWFDGITVYIHSVDAEDKTRVSNVLQRVDSGVRLWNGR"
BASE COUNT          251 a          358 c          597 g          301 t
ORIGIN      
        1 gcggccgttc cagaggcgca cgccggagtc gacgcgttga aggacgttgc tgacgcgggt
       61 cttgtcctcg gcgtcgacgg agtggatgta gacggtgata ccatcgaacc acggtgaggg
      121 accgctggtg ctgacgagat cgccggcgcc gacggcgaag gtttcgggat cggaatcgat
      181 cagcggatcg ttgggattgc cgggagcgaa gaagatgcgg aaaggatcgg ggttcgagag
      241 cgcggaggcg gtgagccagc ggccctggcg ctcgagggtt tcgatcgcgg tgttttttga
      301 accctgtttt tcgaggcgga aggtgcgcac gatggtggtg ccgcctttcg tggcggtgat
      361 cgtcacgtcg ccggtgttgg cgctgaggcc tgtgacggta tagcggttgg cagtcgcgcc
      421 gctgccgaag tttccgctga cgttggtggt ggtggcgctg aacgtccagt tagcagtgtc
      481 ttcgacgcca ccgacgtaga catgcatggt cgtgtaagca tcgttgaaca cagggctcga
      541 accatcggtt cgggtgggaa cgagatggta gtcgttcgag agagcggcgc cgaccgtcga
      601 agatccgtcg gcgacgagga tcgggagaat ttgctcgtcg ataagatagc tgcttccgat
      661 ggtttggtcg tggaggtatt ggcgaacgcg cacgtgtgtg gaggacgcgt tggcgaaatg
      721 agcaagcgac gcggtattgg aactgtaggt cgcgacgtcg gtgaagctgg tgccgttggt
      781 cgattcggcg atgacgtagc gagcggagta aggctgctcc gccgcggtat tagcgcgttg
      841 gtaaccggtg acggtcactt cgacgggcgg gaagagatcg aaactcgcgc cgaggcccag
      901 gccgcgcgcg gtcgttgctg cgctcgggtt gaagatcgac gagtcccaag tcgcgctggt
      961 cggacgcgac gggtagtaaa cggtgctgct ggtggcggtg aggagcgtgc gctcgacggc
     1021 cattttgaag cgcgtgagat cgaaatcgat tttcgcgatc ggacgcggga tgaaattatt
     1081 ggaggggcgg ttgaaaggat agccgttgct gccgtcgccg cggcgcatgt cgtaaaagta
     1141 ggagtcggcg taggtcggga aagcggtcgg caccgtcggg ctggcgtagc cggtgtcggc
     1201 gccgtcgacg ccgccgcctt gcgggatccg gagcacctgg ttgtagccga tggagctgtt
     1261 ggtgcggtag gcgttgggca tccggttcac gtgggcattg aggtcgccgt agctgggctg
     1321 gccggggagg acaacttcct ggatggtgtg ggtgccgtcg gcgttcaccg tgttgaggaa
     1381 tgcgcggtag gagcgcgcgc gcatgatcac ggccgtgccg tcgggcatcc ggccggtgcg
     1441 gttggtgtcg tcgggattga cgacaatgta gaggccgctg cggcgggaga atttggcttg
     1501 gacgagg
//
LOCUS       DASPLT010000764         1354 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_132663, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000764 DASPLT010000000
VERSION     DASPLT010000764.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1354)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1354)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1354
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_132663"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(312..>1354)
                     /locus_tag="VEA63_07935"
     CDS             complement(312..>1354)
                     /locus_tag="VEA63_07935"
                     /inference="COORDINATES: protein motif:HMM:NF019113.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HYD83968.1"
                     /translation="AVQKAWTEKIGLDPQHLVGVKLMDEGATRGWEMATLYVGLLALD
                     AVSEKPVYRRALEHLAEAQHWQLAPRTAHADDLLLGYLYLDLYEREKKTERLAAVQAR
                     VDWLRDHPSPQSLDIRDGQERWTWCDALFMAPPVCLRLWQVTGDRSYLEFMDREWWAT
                     TDFLFSPGDGLFFRDAKFFTQQEANGEKIFWSRGNAWVLASLALMLDDLPADLPSRAR
                     YVALFQTMADRIAALQPADRLWRSSLLDPQANPNPESSSTAFFCYAFARGIRLGLLDA
                     AKYGPIVQRTWAALQRCVHDDGRLGFVQQPGAAPGQADELSTAPYGVGAFLMAGAEVH
                     LLQRQQATASAP"
BASE COUNT          257 a          412 c          444 g          241 t
ORIGIN      
        1 ccccaacggc ctgtcgaagg actggcgcga ctggcagggc aactgcaacg gctacgaggg
       61 ctacctgtgc gatggcttcc tcgcgttgct ggcggtggaa gacgaccttc gcgccggcgg
      121 ataaaacaaa agtgggatgg gcgcgaaacg cgccgggatg atttagctgg attgcctcgt
      181 cgccacgctc ctcgcaatga cacggcggcg gaattgtgaa aactgaaaat cgggcatcgg
      241 gaaactgaga tttattctga ccggctttcc aatttcacgc tgcccgattt caagattacg
      301 ccacgtgatc gtcagggcgc actggcggtg gcttgctggc gctggagcaa gtggacctcc
      361 gcgcccgcca tcagaaatgc gccgacgcca tagggcgcgg tggagagttc gtccgcctgg
      421 cccggcgcgg cgccgggttg ctgcacgaag ccgaggcgtc cgtcgtcgtg cacgcaacgt
      481 tgcagtgccg cccaggttcg ttggacgatc ggtccgtatt tcgcggcgtc cagcagaccg
      541 agcctgatcc cgcgcgcgaa agcgtagcaa aaaaacgccg tgctgctcga ttccggattc
      601 gggttcgcct gcggatccag cagacttgaa cgccacaagc ggtccgcggg ctgcagcgcg
      661 gcgatgcggt ccgccatggt ttgaaacagt gccacgtaac gggcgcggga aggcagatcg
      721 gcgggcaggt cgtccagcat cagcgcgagc gaggcgagca cccacgcgtt gccgcggctc
      781 cagaagattt tttctccatt ggcctcttgc tgcgtgaaga acttggcgtc gcgaaaaaaa
      841 aggccgtcgc cgggcgagaa aaggaagtcg gtcgtcgccc accattcgcg gtccatgaac
      901 tcgagatagc tgcgatctcc ggtcacttgc caaagccgca gacataccgg cggcgccatg
      961 aagagcgcgt cgcaccaggt ccagcgttcc tgaccgtccc ggatatccag cgattgcggt
     1021 gacggatgat cgcggagcca gtcgacgcgc gcctgcacgg ccgccaggcg ttccgtcttt
     1081 ttctcccgct cgtaaagatc gaggtaaagg tagccgagca gcaaatcgtc cgcgtgcgcg
     1141 gtgcggggcg cgagctgcca gtgctgcgct tcggcgaggt gctccagtgc gcgacggtaa
     1201 acgggttttt ccgacacggc gtcgagcgcg agaaggccga catacaacgt cgccatctcc
     1261 caaccacgcg tggcgccttc gtccatcagc ttgacgccga ccagatgttg aggatcgagg
     1321 ccgatctttt ccgtccaggc tttttggacg gcgg
//
LOCUS       DASPLT010000765         1251 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_152668, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000765 DASPLT010000000
VERSION     DASPLT010000765.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1251)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1251)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1251
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_152668"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1251)
                     /locus_tag="VEA63_07940"
     CDS             complement(<1..>1251)
                     /locus_tag="VEA63_07940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="immunoglobulin domain-containing protein"
                     /protein_id="HYD83969.1"
                     /translation="DTETSERHGLYSYNNGVLTTIVDNTATVGTDKLMSISDGVVAND
                     GTVFFQGGGMHATSLYRARNGTIDLFQDGSVGAGAMNPGGMWLHDKYLFFSGNPSAGV
                     TALYRKPIDGTGAATVVFNLSTHPDFKAIDARDIEDLQFANDQVLVRVGAVVKGIQRS
                     ALLYGAASNVLGDSTTTPGSSTPQSQSLALGGSATLSTTATGTLQWQRNGSNLAAATS
                     SSLALSNVQPAAAGIYTVNATNGSTVTAQHAIVGLTTASKVIGTGNEVEANIVHPNGN
                     IFDQVLLTGPAEAITADHTQNQITRTSYIDLDGDIVQVEFSGPGTLSLVLDNPTDPAP
                     PANYNQTVNYMKGHAGIVITGATEQTNVSVFSVGKATAVNQALFKDNVTYDGIADISF
                     IAISSSNGKFGGVRTANANYFAAKG"
BASE COUNT          204 a          311 c          469 g          267 t
ORIGIN      
        1 gcctttcgcg gcgaagtagt tggcgttggc ggtgcgcacg ccgccgaact ttccgttcga
       61 agaggagatc gcgatgaacg agatgtcggc gatgccatcg taggtgacgt tgtctttgaa
      121 gagcgcttga ttgacggcgg tggctttgcc gacgctgaag acggagacgt tggtctgctc
      181 ggtggcaccg gtgatgacga tgccggcgtg acccttcatg tagttgacgg tttggttgta
      241 gttggccggg ggcgcaggat cggtggggtt atcgagcacg agcgagagcg tgcctggacc
      301 ggagaactcg acttgaacga tgtcgccatc gagatcgatg taggaggtgc gcgtgatctg
      361 gttttgcgtg tggtcggcgg tgatggcttc ggcgggaccg gtgaggagaa cttggtcgaa
      421 gatgttgccg ttgggatgaa cgatattggc ttccacttcg ttgccggtgc cgatcacttt
      481 gctcgcggtg gtgaggccga cgatcgcgtg ttgagcggtg acggtggagc cgttggtggc
      541 gttgaccgtg tagatgccgg cggcggcggg ctggacgtta ctgagcgcga gcgaggagct
      601 cgtggcggcg gcgagattgg agccgttgcg ctgccattgg agcgtgccgg tggcggtcgt
      661 gctcaacgtg gcgctgccgc cgagcgcgag ggattgcgac tggggcgtgg acgacccggg
      721 cgttgtcgtg gagtcaccga ggacgttgct cgcagcgccg tagagcaaag ccgaacgctg
      781 gatccctttt accacggcac cgacgcggac gagcacctgg tcgttggcga actggaggtc
      841 ttcgatgtcg cgcgcgtcga tggctttgaa atccgggtgt gtggaaagat tgaagacgac
      901 ggtggcggcg ccggtgccgt cgatcggttt gcgatacaat gcggtcacgc cggcgctcgg
      961 attgccggag aagaaaagat atttgtcgtg cagccacatt cctcccggat tcatcgcgcc
     1021 cgcacccacg ctgccatcct ggaagagatc gatcgtgcca ttgcgagcgc gataaaggct
     1081 cgtggcgtgc atcccgccgc cttggaagaa aaccgtgccg tcgttggcga cgacgccgtc
     1141 gctgatcgac atcagcttgt cggtgccgac cgtcgcggtg ttgtcgacaa tcgtggtgag
     1201 gacgccgttg ttgtaggaat agaggccgtg gcgttcggat gtctcggtgt c
//
LOCUS       DASPLT010000766         1781 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_296335, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000766 DASPLT010000000
VERSION     DASPLT010000766.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1781)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1781)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1781
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_296335"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..827)
                     /locus_tag="VEA63_07945"
     CDS             complement(<1..827)
                     /locus_tag="VEA63_07945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HYD83970.1"
                     /translation="MAKADEAPKIIFSMLGVSKKIERKEILRDISLSFYYGAKIGVLG
                     LNGSGKSSLLRILAGRDTEIEGRLHFEPGYEIGMLEQEPQLTAGSTVRACVEEGVKHL
                     TDLTAAYDATWDELSAAETDEAKDAIANKQAELQDKIDALGAWDVTPQVEMAMDALRC
                     PPGDQLVDKLSGGERRRVALARLLLKKPAILLLDEPTNHLDAESVNWLEQHLARYEGT
                     VIAVTHDRYFLDNVAQWILELSFGHGIPWKGNYSSWLEQKQRRQAVEQRTEDRRQKA"
     gene            1000..>1781
                     /locus_tag="VEA63_07950"
     CDS             1000..>1781
                     /locus_tag="VEA63_07950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HYD83971.1"
                     /translation="MLFSSVTLLSEPSFKTPVSFDDADARATALLTKLTLPQKLGLIS
                     GHATFFIKGYPELGIPELYMSDASGGVNLRRNVNARLDRSTAFPNPLALAATWNPSLA
                     YRYARAIGEECRAGGIAILLGPGMNLYRHSQYGRNFEYFGEDPYLASRLVERYVTGVL
                     DTGTIPTLKHFIGNETDHYRRTSNSLIDDRALHEIYLPAFKTGIDAGAMAVMTAYNLF
                     QGEWCGQNRALITDLLRHELGYKWLVMTDWWSVWDAAKIVRSG"
BASE COUNT          330 a          728 c          384 g          339 t
ORIGIN      
        1 atcgctttct gccgccgatc ttccgtccgc tgctccaccg cctgacgccg ctgcttctgc
       61 tcgagccacg acgaataatt tcccttccac ggaatcccgt gcccaaacga cagctcgaga
      121 atccactgcg cgacgttgtc caaaaaatac cggtcgtgcg ttaccgcaat caccgtcccc
      181 tcgtaacgcg ccagatgctg ctcgagccag ttcacgctct ccgcgtccaa atgattcgtc
      241 ggctcgtcga gcagcaggat cgccggcttc ttcaacaaca accgcgccag cgccacccgc
      301 cgtcgctcac cgcccgacag cttgtcgact aactgatccc ccggcggaca ccgcaacgcg
      361 tccatcgcca tctccacctg cggcgttaca tcccacgcac cgagcgcatc gatcttgtcc
      421 tgcagctccg cctgcttgtt cgcgatcgcg tccttcgctt catccgtctc cgccgcgctc
      481 aactcgtccc acgtcgcatc atacgccgcc gtcagatccg tcaaatgctt cacgccttcc
      541 tccacacacg cgcgcaccgt ggaccccgcc gtcaactgcg gctcctgctc cagcatcccg
      601 atttcataac ccggctcgaa atgcagccgc ccttcaatct ccgtgtcgcg ccccgcgaga
      661 atccgcagca gcgacgactt tcctgatcca tttaacccca gcacgccgat cttcgcgccg
      721 taataaaacg acagcgagat gtcgcgcagg atttccttgc gctcgatctt cttcgagacg
      781 cccaacatcg agaaaatgat cttcggagct tcgtcagctt tagccatgcg cctgaccttg
      841 taggttgcac gcccgcgcgc aaccctcggc aaaacacgtc tcctctccac ctcctttccg
      901 tcattgcagg catcaaacag cggaccgttc catccgcccc cccatctccg atctccaaac
      961 tccgaactcc gatctccgac ccatctccca tctccgaaaa tgctcttctc cagcgtcacc
     1021 cttctctccg agccctcctt caaaacgccc gtcagcttcg acgacgccga cgcccgcgct
     1081 accgcgctcc tcacgaaact cacgctcccg caaaagctcg gcctcatctc cggccacgcc
     1141 acgttcttca tcaagggcta tcccgaactc ggcatccccg agctctacat gtccgacgcc
     1201 agcggcggcg ttaacctccg ccgcaacgtc aacgcccgcc tcgatcgctc caccgccttc
     1261 cccaatccgc tcgccctcgc cgccacctgg aatccatccc tcgcctaccg ttacgcccgc
     1321 gccatcggcg aggaatgccg cgccggcggc atcgcgatcc tcctcggccc cggcatgaac
     1381 ctgtatcgtc attcccaata cggccgcaac ttcgagtatt tcggcgaaga tccctatctc
     1441 gcgtcgcgcc tcgtcgagcg ctacgtcacc ggcgtcctcg acaccggcac gatccccacg
     1501 ctcaaacact tcatcggcaa cgaaaccgac cactaccgcc gcacttccaa ctccctcatc
     1561 gacgaccgcg ccctccacga gatctacctg cccgccttca aaaccggcat cgacgccggc
     1621 gccatggccg tgatgaccgc ctacaatctc ttccaaggcg aatggtgcgg ccagaaccgc
     1681 gcgctcatca ccgaccttct ccgccacgag ctcggttaca aatggctcgt catgaccgac
     1741 tggtggtccg tctgggacgc cgcgaagatc gtccgctccg g
//
LOCUS       DASPLT010000767         1623 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_152683, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000767 DASPLT010000000
VERSION     DASPLT010000767.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1623)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1623)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1623
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_152683"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..483
                     /locus_tag="VEA63_07955"
     CDS             <1..483
                     /locus_tag="VEA63_07955"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HYD83972.1"
                     /translation="PVLTDAVARLVRCLDASGDRVVLVPGILREIVYRLLSSPFGAMI
                     ADFGMAGSRTQRITRAITHLKNRFAEPLRVADLAHLAGMSVSTFHEHFRRITTLSPLQ
                     YQKMLRLQEARRLLVADGAGAADIGFRVGYQSPSQFSREYSRLFGRSPSADMRERMTA
                     "
     gene            598..>1623
                     /locus_tag="VEA63_07960"
     CDS             598..>1623
                     /locus_tag="VEA63_07960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011000070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HYD83973.1"
                     /translation="MKRGRPPKYEVKLSELERETLEQLGRSRSEAAGVVRRAQGLLLA
                     NEGRANDQIAAQLNVNAATVSYWRKRFSEAGLAGLYDLPKAGRPRTHDDERVAVLLKK
                     ALAERPAQATHWSVRQLAAQTGISKSSVQRYFTLFGVQPHRSKAFKLSSDPLFVEKVR
                     DIVGLYLNPPDKALVLCIDEKSQIQALERTQPVLPMGLGYVEGVTHDYYRHGTTTLFA
                     ALEVGTGQVITQCKPRHRHQEFLAFLRHLDRNVPAPLELHLIIDNYATHKHPKVRAWL
                     ARRPRYHVHFTPTSASWLNQVERWFGRITQQSIRRGSFTTVKQLIQRIEAFVHHYNLH
                     PRPFAWTA"
BASE COUNT          320 a          538 c          471 g          294 t
ORIGIN      
        1 ccggtgctga ccgacgccgt cgcccgactt gtccgttgtc tcgacgcgag cggcgatcgt
       61 gtcgtcctgg ttcccgggat tctgcgggag atcgtttacc gactgctttc gagtccattc
      121 ggcgccatga tcgcggactt cggcatggcg ggaagtcgga cgcaacgtat cacccgagcc
      181 attacgcatc tgaaaaaccg attcgccgag ccgctgcgcg tcgctgatct cgcccacctc
      241 gcggggatga gcgtctccac ctttcacgaa cacttccgcc ggatcacgac gctgagtccg
      301 ctccaatacc agaaaatgct ccgcttgcag gaagcgcgac gactcctcgt ggcggacggc
      361 gcgggcgcgg ccgacatcgg cttccgcgtc ggttaccaga gtccgtcgca atttagccgc
      421 gagtattccc gactgtttgg tcggtcgccc tcggcggata tgcgcgagcg aatgaccgcg
      481 tagcgccgac cgcaaagggc aacattcgat tcgaggccga ccttcctcgg atatttcacg
      541 ctcactagcg gcgtgtcccg caagtaacat agttaaagaa cggcggtctg aaaagggatg
      601 aaacgaggac gaccaccaaa gtatgaggtg aagttaagcg agctggagcg ggagacattg
      661 gagcagttgg ggcgatcacg cagcgaggcg gcgggagtgg tgcgccgagc gcaaggcttg
      721 ttgctggcca acgaaggtcg tgcaaacgac cagatcgccg cgcaattgaa cgtcaacgcc
      781 gcgacggtct cctactggcg caaacgtttt agcgaagcgg gattggctgg cttgtatgac
      841 ctgccgaagg caggccggcc gcgcacgcac gacgacgagc gcgtggcggt gctgctcaaa
      901 aaggcgttgg cggagcgacc ggcgcaggcc acgcactgga gcgtgcgaca actggccgct
      961 caaacgggca tctccaagag cagcgtgcag cgttacttca cgttgtttgg cgtgcagccc
     1021 caccgcagca aggccttcaa gctttcgagc gatccgctct tcgtcgagaa ggtgcgcgat
     1081 atcgtggggc tgtatctcaa tccgccggac aaggcgctgg tgctctgcat cgatgagaag
     1141 agccagatcc aggctctgga gcgcacgcag ccggtgctgc cgatgggctt gggctacgtc
     1201 gaaggcgtca cccacgacta ctaccgacac ggcaccacga ctttgtttgc cgcactcgaa
     1261 gtcggcaccg gccaggtcat cacccaatgc aagccgcgcc atcgccatca agagttcctc
     1321 gcgttcctgc ggcaccttga ccgcaacgtc cctgcgccac tcgaactgca cctcatcatc
     1381 gacaactacg ctacccacaa gcatccgaag gtccgcgcct ggctcgctcg ccgcccccgc
     1441 taccacgtcc acttcacccc cacctccgct tcctggttaa atcaggtcga gcgctggttt
     1501 ggccgcatca cccagcaatc cattcgccgc ggctccttca ccaccgtcaa acaactcatc
     1561 cagcgcatcg aagccttcgt ccatcattac aaccttcacc ctcgtccctt cgcctggacc
     1621 gcc
//
LOCUS       DASPLT010000768         2097 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_33174, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000768 DASPLT010000000
VERSION     DASPLT010000768.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 2097)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2097)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2097
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_33174"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..508
                     /locus_tag="VEA63_07965"
     CDS             <1..508
                     /locus_tag="VEA63_07965"
                     /inference="COORDINATES: protein motif:HMM:NF019247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1579 domain-containing protein"
                     /protein_id="HYD83974.1"
                     /translation="PSLDECAQMFATPHPEHAWLQKLVGNWSFESECMGEPGQPTEKQ
                     RGTETVKALGGLWIVGEGQGGMPGGGTANMMITLGFDPQRNRFVGTWVGSMMTHLWVY
                     EGELDAAKRVLTLNAEGPSFADPAKRAKYQDIIEIKSDDHRTLSSRTLGEDGKWTHFM
                     TAHYRRTK"
     gene            724..>2097
                     /locus_tag="VEA63_07970"
     CDS             724..>2097
                     /locus_tag="VEA63_07970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007363291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HYD83975.1"
                     /translation="MTTERKLLAINAAYLALGLCIAALAIFTERTQLLLTKANENRFV
                     SYQLANELKQSSEDLTRLARTFVVTGEPRYEEAYWDVLAVRNGKKARADGTTLSLREL
                     MERAGFTAEEFAELKKAEDNSNALVTTETIAMNAVKGFYDDGNGNYTKQAAPDAELAR
                     RIMHDEKYHENAALIMAPIHVFETMLDSRTRSEVEKHAQRRDLLFWSTVATIPVILML
                     GVGGVWMIKFRIARPLNTVIANLHGTANTIGNAIEQIAAAGASLANGATTQAASLEET
                     SASLEEMSGMTKRNAEHAQAANALTKQTRASAETGAGDMQEMTTAMDAIKAASDNIAK
                     IIKTIDEIAFQTNILALNAAVEAARAGEAGMGFAVVAEEVRNLAQRSAEAAKETAARI
                     EDSIQKSERGVAISAKVADSLGEIVTKARKVDELVAEIASASLEQSQGITQLNTAVTQ
                     MDRVTQAN"
BASE COUNT          477 a          614 c          686 g          320 t
ORIGIN      
        1 cccatcgctg gatgaatgcg cgcagatgtt tgccacaccg catccggagc acgcgtggct
       61 gcaaaagctc gtcggcaatt ggtcgtttga atccgaatgt atgggcgagc ccggccagcc
      121 caccgagaaa cagcgcggaa ccgaaacggt gaaggcgctc ggtggtttgt ggatcgtcgg
      181 cgaaggccag ggcggaatgc ccggcggcgg caccgcgaat atgatgatca cgctgggctt
      241 cgatccgcaa cgaaaccggt tcgtcggcac gtgggtggga tcgatgatga cgcacctctg
      301 ggtctacgag ggcgaactcg atgcggcgaa gcgcgtgctc accctcaacg ccgagggccc
      361 gagtttcgcc gatccggcaa agcgtgcgaa atatcaggac atcatcgaaa tcaagagcga
      421 cgaccaccgg accctctcgt cacgcacgct cggcgaagac ggcaagtgga cgcacttcat
      481 gaccgcgcat taccggcgaa cgaagtagtc gccctctcgc atgatcgcgc ggccggtcgc
      541 gcgattgaaa gtgccattga acggcgatgc gaaggccgat gttcaatgac gtcgcgacca
      601 cgagcggatt tctccaccat cgccggagaa acgctcaaga tcggttctcg gcaacccgat
      661 tagagtaggg accgggaccg cgccagagct tcagcaattg ggcgggtcct ggctcgacct
      721 ctcatgacta cggaacgaaa gctcctggcg atcaacgcgg cctacctcgc attgggcctt
      781 tgcatcgccg cactcgcgat tttcacggaa cgcacgcagt tgctgctgac caaggcgaac
      841 gagaatcgtt ttgtgtccta ccagcttgcg aacgagctca aacaaagctc ggaagatctc
      901 acgcgcctgg cgcggacgtt tgtggtgacc ggcgaacccc gttatgaaga ggcgtattgg
      961 gacgtcctgg cggtgcgaaa cggaaagaag gcgcgggccg atggcacgac cctgtcgttg
     1021 cgcgaactca tggagcgagc cggctttacc gctgaggagt tcgcggagct gaagaaagcg
     1081 gaggacaact ccaacgcgct cgtgacgacc gagacgatcg cgatgaacgc ggtgaagggc
     1141 ttctacgacg acgggaacgg aaattatacg aagcaggcgg cgcccgatgc ggagctcgct
     1201 cgccggatca tgcatgacga aaagtatcac gagaacgcgg cgttgatcat ggcgccaatc
     1261 cacgtgttcg aaacgatgtt ggattcgcgc acgcggtcgg aagttgaaaa gcacgcccag
     1321 cggcgcgacc tgctgttctg gtcgacggtg gcgacgatcc cggtcatttt gatgctcggc
     1381 gtcggcggcg tgtggatgat caagtttcgg atcgcgcgac cgctgaacac tgtgatcgcc
     1441 aatctgcacg gcaccgcgaa tacgatcggc aacgcgatcg agcagatcgc ggccgcgggt
     1501 gcgtcgctgg caaacggcgc gaccacgcag gctgcctcgc tcgaggaaac cagcgcctcg
     1561 ctcgaggaaa tgtccggcat gacgaagcgg aacgccgagc acgcccaggc ggccaacgcc
     1621 ctgaccaaac agacgcgcgc ttcggcggag accggcgccg gcgacatgca ggagatgacc
     1681 acggcgatgg atgcgatcaa ggcggccagc gacaacatcg cgaaaatcat caagacgatc
     1741 gatgagatcg cgtttcagac gaacatcctg gcgctgaacg cggcggtcga ggccgcgcgc
     1801 gcgggcgaag ccggcatggg cttcgccgtg gtcgcggaag aagtgcgaaa cctggcgcaa
     1861 cgcagcgcgg aagcggcgaa ggaaactgcc gcgcgaatcg aagactcgat ccagaaaagc
     1921 gaacgcggcg tggcgatcag cgcgaaagtc gcagacagtc ttggcgaaat cgtgaccaag
     1981 gcccggaagg tggacgagct cgtcgcagaa attgcgtctg cctccctcga gcaaagccag
     2041 ggtatcacac agctgaatac ggcggtgacc cagatggacc gcgtaacgca ggcaaac
//
LOCUS       DASPLT010000769         1606 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_110997, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000769 DASPLT010000000
VERSION     DASPLT010000769.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1606)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1606)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1606
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_110997"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..451
                     /locus_tag="VEA63_07975"
     CDS             <1..451
                     /locus_tag="VEA63_07975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD83976.1"
                     /translation="TTALLDAIGHTVDRLGQRLANTPERERPSQVIVAILTDGLENAS
                     TRYTWADIATRIRHQQEKYNWRFLFLGANQDAIATAARLNIGGADAATFRASDKGLAA
                     STRAVTRRTTAIRQAARGHLVGADLSKPMQTILAEEDLGAAAPMNQP"
     gene            548..859
                     /locus_tag="VEA63_07980"
     CDS             548..859
                     /locus_tag="VEA63_07980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD83977.1"
                     /translation="MMTTYSSRSLRRLRLIDRRTTASHVAARNVALFFRDLASFVTFL
                     LRTTLLALLFLPALLGAVGALTWNALTSVLVDRSRRPRSLSFITVSGFLLGVVLGRSC
                     R"
     gene            complement(1027..1287)
                     /locus_tag="VEA63_07985"
     CDS             complement(1027..1287)
                     /locus_tag="VEA63_07985"
                     /inference="COORDINATES: protein motif:HMM:NF015630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0175 family protein"
                     /protein_id="HYD83978.1"
                     /translation="MTVAVSLPEEVATLAKSEGGELDRAVAVTIVFYHYERGRLSAGK
                     AAELLGVARAEFEQLRVERGIERPFTGDDLERDLAWAKKRAG"
BASE COUNT          303 a          572 c          443 g          288 t
ORIGIN      
        1 caccaccgcg ctcctcgacg cgattggtca cacggtggac cgactcgggc aacgcctcgc
       61 gaacacgccc gaacgcgagc gccccagcca ggtcatcgtc gcgatcctga cggacggact
      121 cgaaaacgcg tcgacccgct acacttgggc ggacatcgcc acgcgcatcc gtcatcaaca
      181 ggaaaaatac aactggcgat tcctcttcct cggcgcgaat caggacgcca ttgccaccgc
      241 cgcgcgttta aacattggcg gcgccgacgc cgccaccttt cgggcgagcg acaaaggcct
      301 tgccgccagc acgcgcgccg tcacccgccg cacgaccgcg atccggcagg ccgcccgcgg
      361 ccacctcgtc ggcgcggatc tttccaagcc catgcagacg atcctcgccg aggaagattt
      421 gggcgccgct gcgccgatga atcagccctg acccaagccc gaagtgacgc ccgctcgcgt
      481 agtctcgatt ccaacaacgc ctccctgcac cgacgattcg cgccccgaga atcacgccac
      541 gcttcccgtg atgacgactt actcttcaag atcgctgcgc cgcctgcgac tgatcgatcg
      601 caggaccaca gccagccacg tggccgcgcg aaatgtcgcc ctcttcttcc gcgatctcgc
      661 aagcttcgtg acgttcctac ttcggaccac gctcctcgcc cttctgttct tacccgcgct
      721 gctgggcgcg gtgggcgcgc tgacctggaa tgcactgacg agcgtgctcg tggatcgttc
      781 acgacgcccg cgatcccttt cattcatcac cgtctccgga tttctgctcg gcgtcgtgct
      841 gggccggagc tgcaggtaac gttgacccca tcgttatttg ttatgtccga agctttcgat
      901 tcgccgatgt caaccagcac gcgtcacctg ccccgcgggc ccgactgcaa accgggcttc
      961 aaccgcaaaa tcaatagcgg gtatcgtgct ggcgaagtcg caaatcgccc cgctgccacg
     1021 gccggttcac ccagcccgtt ttttcgccca agccagatcc cgctccaggt cgtcacccgt
     1081 gaagggccgt tcaattccac gttcgacgcg caattgctca aactccgccc gggcgacacc
     1141 gagcagttca gccgccttgc ccgccgaaag ccggcctcgt tcgtagtggt agaaaacaat
     1201 cgtgacggcg accgcacggt ccagctcgcc accttcgctt tttgcgaggg tggcgacttc
     1261 ttccggcagc gaaacggcga cggtcatgat ggaggaagca atgcgaaacg tgcgaagatt
     1321 cgcaaggctt ggcttcaacg gagccgcgct ctttcgagcg cggaagctag cgatatttgg
     1381 gaatgcgaca tatccctcac tcgacttcaa cggggccacg ctctcgcgag cgcggaaaaa
     1441 cgaaacggct ccagtttggc atcagacttc ccccatttgc tggtagaatc tgcggagcaa
     1501 gctgggactg aaaaatcggc ccgcttcccg cattgcctcc gcgacgggcc tgacgcgcgc
     1561 tatgacgccg gcgtgcttgc acgcggccag gatgcccaga ctccca
//
LOCUS       DASPLT010000770         1707 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_234428, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000770 DASPLT010000000
VERSION     DASPLT010000770.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1707)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1707)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1707
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_234428"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..904
                     /locus_tag="VEA63_07990"
     CDS             <1..904
                     /locus_tag="VEA63_07990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD83979.1"
                     /translation="AAPSPPATEAPKFKLKVKPAEPAAASSPPPPVAPTPPTPPPSPA
                     AAAPKPPSPPAFPVVAPADTPPSQTAPSPPPPTAGSSAPPVSDNADKNRPAVAPHGPK
                     KSLSKILVVGGVTLVAAAAAGYYFLFAPDPEPLPPPPAKASTPKPTPSDTLNKIAATP
                     AQSINKAKDVIAARRATDEQGIDAVLESEASTSDPASTKKQPPAAAPIRATSQLSPGV
                     VATTTVANISGNASPAFSTWVANAKISGVFQGTPPRALINGRTVRLGQEVDIALGISF
                     HALDAAAKTIVFRDQSGATVSRRY"
     gene            complement(950..1627)
                     /locus_tag="VEA63_07995"
     CDS             complement(950..1627)
                     /locus_tag="VEA63_07995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family NAAT transporter"
                     /protein_id="HYD83980.1"
                     /translation="MLSATRFWELILGTVIALLPVINPLASASTFLAITEGDTRERRM
                     QQLRMACFYMVAILVSFLLGGTFIMSFFGLSIPGLRIAGGLLVAGIGSKMLMAPPSVP
                     DDDPVHAAARAKQDVSFSPLAMPMMSGPGSIAMTVGFTSLASGWLDYVAIILGIVVVA
                     VITFVTLALSDRVVRVIGVNGMNALSKIMGFIILCIGVQFVVNGVLEIVTDPELVRGL
                     RDAAASE"
BASE COUNT          358 a          679 c          424 g          246 t
ORIGIN      
        1 agccgcccct tctccgccag cgaccgaagc cccgaagttc aagctgaagg tcaagcccgc
       61 cgaacccgcc gccgcgtcca gccctccgcc ccccgttgcg ccaacacccc caaccccgcc
      121 gccaagccct gcggcagcag cccccaaacc gccgagtccg cccgcatttc cggtcgtcgc
      181 tccggccgac accccgcctt cccaaaccgc accctccccg ccgccaccga ccgcgggaag
      241 cagcgcgccg cctgtcagcg acaacgccga caaaaaccgc cccgcagtcg caccacacgg
      301 accgaagaaa tcgctttcca aaatcctggt cgtcggcggc gtcaccttgg tcgccgccgc
      361 agccgccggc tattatttcc tcttcgcgcc cgaccccgag ccccttccgc cgccgccagc
      421 caaagcctcg acgcccaagc cgactccatc cgacacgctg aacaaaatcg ccgcgacgcc
      481 tgcccaaagc atcaacaaag ccaaggacgt catcgccgcc cgtcgtgcga ccgacgagca
      541 aggcatcgat gccgtgctgg aaagcgaagc ttccacctcc gaccccgcat ccacgaagaa
      601 gcagccaccg gcagccgccc cgatccgggc gacctctcaa ctctcccccg gcgtcgtcgc
      661 gaccacgacc gtggccaata tctccggcaa cgcctcgccc gcatttagca cttgggtcgc
      721 caacgcgaaa atcagcggcg tcttccaagg cacgcccccg cgcgctctga tcaacggccg
      781 caccgtccgg ctcgggcagg aggtcgacat cgcgctcggc atctcgtttc acgccttgga
      841 cgccgccgcc aagacgatcg tgtttcgcga tcaatccggc gccaccgtca gccgccgata
      901 ttgatcgccg ccttgctgga cgctccgctg ccctgcgctc atccgtcgct cactcgctcg
      961 ccgccgcatc gcgcaaccca cgcaccagct ccggatcggt cacgatctcg agcacgccgt
     1021 tcaccacgaa ctgcacgccg atgcacagga tgataaatcc catgattttc gagagcgcgt
     1081 tcatgccatt gacgccgatc acccgaacca cgcgatccga caacgcgagc gtcacgaacg
     1141 tgatcactgc gaccaccacg atgcccagaa taatggcaac gtaatcgagc cagccgctcg
     1201 cgagcgaggt aaaccccacc gtcatcgcga tcgaacccgg tccgctcatc atcggcatcg
     1261 ccagcggcga aaacgacacg tcctgctttg cgcgcgccgc cgcgtgcacc ggatcgtcat
     1321 ccggcacgct cggcggcgcc atcaacattt tcgagccgat ccccgccacc aacaatccgc
     1381 cggcgattcg cagccccgga atcgaaagcc cgaaaaaact catgatgaac gtcccgccca
     1441 aaagaaaact cacgagaatc gccaccatgt agaaacacgc catccggagc tgctgcatcc
     1501 gccgctcgcg cgtgtcgcct tcggtgatcg cgagaaacgt cgacgccgac gcgagcggat
     1561 tgatcaccgg caacagcgcg atgaccgtgc caaggatcaa ctcccagaag cgcgtggcgg
     1621 atagcatgac gaacaagttt tgggttttga gttttgggtt acgccagcgc cactccgctt
     1681 ctcaactcat aactcataac tcataac
//
LOCUS       DASPLT010000771         1126 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_69596, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000771 DASPLT010000000
VERSION     DASPLT010000771.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1126)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1126)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1126
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_69596"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..466)
                     /locus_tag="VEA63_08000"
     CDS             complement(<1..466)
                     /locus_tag="VEA63_08000"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HYD83981.1"
                     /translation="MKPAAQTSSSDVVLEVDELRTHFITEEGGALPAVDGVSFSIQRG
                     RTLALVGESGCGKSVTSFSLLRLIQRPGKIVGGRVVLHSSRQGEIDITSLPEKSRRLR
                     QVRGGLVSMIFQEPMTALSPVHTIGSQIAEVILLHRGVSREEARRRGIDMLRK"
     gene            complement(463..>1126)
                     /locus_tag="VEA63_08005"
     CDS             complement(463..>1126)
                     /locus_tag="VEA63_08005"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HYD83982.1"
                     /translation="HDIFSRLVYGARISLSVGVVAIAVTFLLGVTIGGVSGYVGGSTD
                     NVIQRLIEIVNAFPQLPLWLALGAVLPPDWSPLTVYFAITIVLSLLGWTALARVVRGK
                     ILSLREEDYAVAARLIGASHFRILFRHLLPGFTSHIIVVLTMSVPAMILGETSLSFLG
                     LGLRPPIVSWGVMLQDCMSIQTVANYPWLLFPVVLIVLTVLAFNFLGDGLRDAADPYS
                     GR"
BASE COUNT          242 a          389 c          327 g          168 t
ORIGIN      
        1 ccttgcgcaa catgtcgatg ccgcggcgcc gcgcttcctc ccgcgagaca ccacggtgca
       61 gcaggatgac ttccgcgatc tggctgccaa tcgtatgcac cggtgacagc gccgtcatcg
      121 gctcctggaa aatcatgctc accagcccgc cgcgcacctg ccgcagtcgg cgcgattttt
      181 ccggcaaact cgtgatgtcg atctcgcctt ggcgcgacga atggagcacc acgcgcccgc
      241 cgacgatttt tcccgggcgt tggatcaacc gcagcagcga gaaactcgtc accgattttc
      301 cgcaacccga ctcgccgacg agcgcgagcg tccgaccgcg ctggatcgaa aacgacacac
      361 cgtccaccgc cggcagcgcc ccgccctctt ccgtgatgaa atgcgtgcgc agttcgtcga
      421 cttcgaggac gacatccgaa gaggaagttt gggcggcagg tttcatcggc cggaataagg
      481 atcggcggcg tcgcgcagtc cgtcgccgag gaagttgaag gccagcaccg tcagcacgat
      541 cagcacgacg ggaaacagca gccacgggta gttcgccacc gtttggatgc tcatgcagtc
      601 ctgcaacatc acgccccagc tcacgatcgg cggtcgcagg cccagcccga gaaacgacag
      661 cgacgtctcg ccgagaatca tcgccggcac gctcattgtc agcacgacga tgatgtgact
      721 cgtgaaaccc ggcagcagat gccggaagag tatccgaaaa tgcgatgcgc cgatcagccg
      781 cgccgccacc gcgtaatctt cctcgcgcaa cgacaatatt ttcccgcgaa ccacccgcgc
      841 cagcgccgtc cagcccagca ggctcaacac gatcgtgatc gcgaaataaa ccgtgagcgg
      901 cgaccagtcc ggcggcagca ccgcgccgag cgcgagccag agcggcagtt gcggaaacgc
      961 gttaacgatt tcgatcaacc gctggatgac gttgtccgtc gaaccgccga cgtagccgga
     1021 gaccccgccg atcgtcacgc cgagcaggaa cgtcaccgcg atcgccacga cgccgaccga
     1081 caggctgatg cgcgccccgt aaacgagccg gctgaaaata tcatga
//
LOCUS       DASPLT010000772         1428 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_152803, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000772 DASPLT010000000
VERSION     DASPLT010000772.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1428)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1428)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1428
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_152803"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(46..888)
                     /locus_tag="VEA63_08010"
     CDS             complement(46..888)
                     /locus_tag="VEA63_08010"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008758 - UDP-2,3-diacylglucosamine
                     hydrolase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HYD83983.1"
                     /translation="MKSLLRVRTVILSDVHLGTPDCKIDEVNHFLRHTRCARLILNGD
                     IIDGWQLRRSGQWTKAHTRFVRIVLKKLEKRDTEIVYLRGNHDDILSSFLPLDFENLQ
                     IVEDYIHETPRGRYLVLHGDVFDTITKNFVFLAHLGDWGYRALLRINRLYNVWRAWRG
                     KEYWSLSKAIKARVKEAVSHVSKFEDHIAELARSRGCTGAMCGHIHTPADKMLGDIHY
                     LNSGDWVESLTAIVEHWDGRFELIAFADFVRDYPMSSTEPEPAIDEIASEAPDLAHHQ
                     SLQR"
     gene            complement(885..>1428)
                     /locus_tag="VEA63_08015"
     CDS             complement(885..>1428)
                     /locus_tag="VEA63_08015"
                     /inference="COORDINATES: protein motif:HMM:NF039905.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD83984.1"
                     /translation="LPAYARTGLTAFFRRRLQPVVIQANGRRHTLDTLLVAVANSDQY
                     GNGARIAPGARIDDGQLDLVAVQPVGFGSALPLVARLFLGSFHRSPRVLHLRSHRFVI
                     ERRAPDLIHTDGETHATGAAVEIAVHPRSLRLLVPAARGPRVHAALPAQSNPAACEPR
                     RLIGHTTPSPLFQAVGSFSP"
BASE COUNT          268 a          382 c          515 g          263 t
ORIGIN      
        1 aagatcggtt gaagcggcgc gagcagaaat gccgcgcgcg gaggcctatc tttgtagtga
       61 ctgatgatgc gcgaggtcgg gcgcttcgga tgcgatttcg tcgatcgcgg gttcgggctc
      121 ggtgctgctc atcgggtagt cgcgaacgaa atcggcgaag gcgatcaatt cgaagcggcc
      181 gtcccagtgc tcgacgatcg ccgtgagcga ttcgacccaa tcgcctgagt tcaggtagtg
      241 aatatcgccg agcatcttgt cggcgggcgt gtgaatgtgg ccgcacattg cgccggtgca
      301 gccgcgcgag cgtgcgagct cggcgatgtg atcctcgaat ttcgagacgt ggctcaccgc
      361 ttccttcacg cgggctttta tggctttgct gagcgaccag tattccttgc cgcgccaggc
      421 gcgccacacg ttgtagaggc gattgatccg cagcagcgcg cgataacccc agtcgccaag
      481 atgcgcgagg aaaacgaagt tcttcgtgat cgtgtcgaac acatcgccgt gcagcacgag
      541 gtagcgaccg cgcggtgttt cgtgaatgta gtcttcgacg atctggagat tttcgaaatc
      601 gagcggaagg aaggaggaga ggatgtcgtc gtgattgccg cgcagataca cgatctcggt
      661 gtcgcgcttc tcgagtttct tgaggacgat gcggacgaag cgcgtatggg ctttggtcca
      721 ttgtcccgag cggcgcaact gccagccgtc gataatgtct ccgttgagga tgaggcgcgc
      781 gcagcgggtg tggcggagga agtgattcac ctcgtcgatt ttgcaatcgg gcgtgccgag
      841 gtgcacgtcg gagagaatca cggtgcgaac gcgaaggagc gatttcatgg cgaaaaagag
      901 ccgaccgcct gaaacagcgg cgacggagtc gtgtgaccta tcaggcgacg cggttcgcag
      961 gccgccgggt tggactgcgc gggcaatgct gcgtgaacgc gtggtccgcg cgccgcgggg
     1021 acgaggagtc gcaggctgcg cgggtgcacg gcgatctcga ccgcggcgcc ggtggcgtgc
     1081 gtctcgccat ccgtgtggat caagtcaggt gcgcgtcgct cgatgacaaa gcgatggctg
     1141 cgaagatgga gcacacgtgg gctgcggtga aagctgccga gaaacaagcg tgcgaccagc
     1201 ggcagcgcac tgccgaaacc gacgggctgc accgcgacga gatcgagttg tccgtcatcg
     1261 atgcgggcac ccggtgcgat gcgggcgccg ttgccatatt ggtcggagtt ggcgacggca
     1321 accagcagcg tatccagcgt gtggcgacgg ccgttcgctt ggatgacgac gggttgcaag
     1381 cggcggcgga aaaatgcggt caggccggtg cgcgcatacg ccgggagg
//
LOCUS       DASPLT010000773         1597 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_173806, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000773 DASPLT010000000
VERSION     DASPLT010000773.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1597)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1597)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1597
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_173806"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..656)
                     /locus_tag="VEA63_08020"
     CDS             complement(<1..656)
                     /locus_tag="VEA63_08020"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HYD83985.1"
                     /translation="MTRPLPRILLVEDDRQLIYLVRVTLERKGYRVFEAFDGPSGLES
                     ALKERPDLLVLDIDLPGLNGLEVCEELRRLHYSAPILMLTGRAQLDEKVAGLESGADD
                     YLAKPFEPREFLARVQALLRRHQRDEAPTPTLDLGDVRIDLRERTATRNGQALTLTKT
                     EFGLLELLAKTPGQPVSRETILDIVWGYARFPTTRTVDTHIWRLRKKIGDDGDEPRWI
                     "
     gene            872..>1597
                     /locus_tag="VEA63_08025"
     CDS             872..>1597
                     /locus_tag="VEA63_08025"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HYD83986.1"
                     /translation="MRQTLCGLLCATLTAPAYADGVRNLGDLSIEELLNESVTSVSKR
                     EQKLGDVASAIAVLSNDDLRRSGATTIADALRLVPGVYVGSVSASQWSVSARGFNGLY
                     ANKLLVLVDGRAVYTPIFAGVYWDVQQPMLEDVDRVEVIRGPGASVWGANAVNGVINV
                     VSRSARDTQGSLVYGGGGEGQRALAGVRHGDRAGENTYYRVYASHQANDGFVLANGAD
                     AGDTWHGWTSGFRVDHYPEPATHL"
BASE COUNT          258 a          449 c          589 g          301 t
ORIGIN      
        1 ttgatccagc gcggttcgtc gccgtcgtcg ccgattttct tgcggagccg ccagatgtgg
       61 gtgtcgaccg tgcgcgtggt ggggaagcgc gcgtagcccc agacgatgtc gagaatggtt
      121 tcgcgggaga cgggctggcc gggagttttc gcgagcagtt cgagcaggcc gaattccgtt
      181 ttggtcaacg tcagcgcttg gccgttacgt gtggcggtgc gttcgcgcag atcgatccgc
      241 acgtcgccga gatcgagggt tggggtgggg gcttcgtcgc gttgatggcg gcgcagcagc
      301 gcttgcacgc gcgcgagaaa ctcccgcggc tcgaaaggtt tggcgaggta gtcgtcggcg
      361 ccggattcga ggccggcgac cttctcgtcg agctgcgcgc ggccggtgag catgaggatc
      421 ggcgcgctgt agtggagccg gcgcaactcc tcgcacacct cgaggccgtt caagccgggc
      481 aggtcgatgt cgagcacgag caaatcgggc cgctccttga gcgcggattc caagccggaa
      541 ggtccgtcga atgcctcgaa cacccgatag ccttttcgtt cgagcgtgac gcgaacgaga
      601 tagatgagct gacggtcgtc ttcgaccaga agaatacggg gtagagggcg ggtcatggat
      661 gcggggcgca agaccccggc ggcacgacgc taggcgcggg gagatcgcgc gcaactctgc
      721 cgatcgacgc gctggcattt tcgcgctgaa acccggccgc gtttgtgacg ggttcgtgac
      781 accttcgcag ccgatttgcg cgatgatgat tgtctccatg aagactcatc gccgcggagg
      841 tgccgctggc tccggaaaat tttcaaccct cttgcgccag acgctttgcg gactgttgtg
      901 cgccacgctg actgcaccgg cgtatgccga tggcgtgcgc aatctcggcg acttgagcat
      961 cgaggaattg ctcaacgagt cggtgacctc ggtgtcgaag cgcgagcaga aactcggcga
     1021 cgtggcatcg gcgatcgcgg tgttgtcgaa cgacgatctg cggcgctcgg gtgcgacgac
     1081 gattgcggat gcgttgcggc tggtgccggg cgtctacgtg ggctcggtga gcgcgagcca
     1141 gtggtcggtg tcggcgcgcg gtttcaacgg gctttacgcg aacaaactcc tcgtgctggt
     1201 cgacggtcgc gcggtttata cgccgatttt cgcgggggtt tattgggacg tgcagcagcc
     1261 gatgctcgaa gacgtggatc gtgtcgaagt gatccgcggg ccgggtgcga gcgtgtgggg
     1321 tgccaacgcg gtgaacggcg tgatcaacgt cgtctcgcgc agcgcgcgcg acacgcaagg
     1381 ttcgctcgtc tacggcggcg gcggagaggg gcagcgcgcg ctcgcggggg tgcgtcacgg
     1441 ggatcgcgcc ggcgagaaca cctactatcg cgtttacgcg agccatcagg cgaacgacgg
     1501 gttcgttctc gcgaatggcg cggacgcggg ggacacgtgg cacggatgga cgagtggatt
     1561 tcgcgtcgat cattatcccg agccggcgac gcatctc
//
LOCUS       DASPLT010000774         1624 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_33274, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000774 DASPLT010000000
VERSION     DASPLT010000774.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1624)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1624)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1624
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_33274"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..205)
                     /locus_tag="VEA63_08030"
     CDS             complement(<1..205)
                     /locus_tag="VEA63_08030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD83987.1"
                     /translation="MRASRSILFASIAVMLCAAAFLGWVHALRVAHVDHVSAIMDEAP
                     RVDARSPTGLAGETRTLIVPAHHR"
     gene            237..>1624
                     /locus_tag="VEA63_08035"
     CDS             237..>1624
                     /locus_tag="VEA63_08035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HYD83988.1"
                     /translation="MGTKKVQRPPASTLSFLTVLTLLLNVRCSPAPPSSMTEFATPNR
                     PALHARRIPRWRGAAAIALAALVNATVYAADSSAAEEIETHPLAAISGPRGNTLFTLL
                     KPEETGFLAENRYADPRIWGERFREFEMGAIGTGIAVADYDDDGRPDIFVVSKTESSR
                     LFRNLGDWKFEDVTERAGIADHGAAAQIWKQGAAFSDVDNDGWLDLYVCRFDAPNLLY
                     INQGDGTFVEEAARRGLAVVDASGMAAFCDYDRDGWLDVYLQTNLYKNAERPDGQRDY
                     LFRNNGDGTFTNVTDRAGIRGETQGHSATWWDYDRDGWPDLYVANDFAPADQLYRNNG
                     DGTFSDVLDQVVPHTPYSSMGSDLGDINNDGLIDFFVADMATTTHAKDLRTMAEVRAR
                     ARDPRDRAQAPQYTRAALYVNTGVGRLLEGALLAGVAATDWAWSVRFEDLDNDGRLDL
                     HVTNGMFREIHNV"
BASE COUNT          320 a          558 c          475 g          271 t
ORIGIN      
        1 cccgatgatg cgccggcacg atcagcgtcc gcgtctcgcc cgcgagtccc gtcggcgacc
       61 gggcatccac ccgcggcgct tcgtccatga ttgccgaaac gtgatcgacg tgcgccaccc
      121 gcaacgcatg gacccacccc aaaaatgccg cggcacacag catcaccgcg atcgaggcaa
      181 agaggatcga gcgagaggcg cgcatgcgat ttaccacgct gaacgacatt tcaccgatgg
      241 gaacgaagaa agtgcagcgc ccgcccgcaa gcacgttgtc cttcctcacc gtcttgaccc
      301 tgctgctcaa cgtcagatgt tcccccgccc cgccctcgtc catgactgag ttcgccaccc
      361 cgaatcgacc cgccctgcac gcgcgacgta tcccacgttg gcgaggcgct gccgccatcg
      421 cgctcgccgc gctcgtcaac gcgacagttt atgcggcgga ttcctccgca gccgaggaaa
      481 tcgaaacgca tccgctggcg gcaatttccg gaccgcgcgg caacacgctg ttcacattgc
      541 tcaagccgga ggaaacggga ttcctcgccg aaaaccgcta cgccgatccg cgcatttggg
      601 gcgagcgatt ccgcgagttc gaaatgggcg cgatcggcac cggcatcgcg gtcgccgact
      661 acgacgacga cggtcggccc gacattttcg tcgtgagcaa aacggaaagc tcccgcctct
      721 ttcgcaatct cggcgattgg aaattcgagg acgtcaccga gcgcgccggc atcgccgatc
      781 acggtgccgc tgcgcaaatt tggaaacagg gtgcggcgtt ttccgacgtc gacaacgacg
      841 gttggctcga cctctacgtc tgccgcttcg acgcacccaa ccttctctac atcaatcaag
      901 gcgacggcac gttcgtcgag gaggccgcgc ggcgcggcct cgccgtcgtc gacgcaagcg
      961 ggatggcggc tttttgcgac tatgatcgcg acggctggct cgacgtttac ctccagacaa
     1021 atctctacaa aaacgcggag cggccggatg ggcagcgcga ctatcttttc cgcaacaacg
     1081 gcgacggcac gtttacgaac gtcacggatc gcgccggcat ccgcggcgaa acccagggcc
     1141 actccgcgac gtggtgggac tacgatcgcg atggctggcc ggacctctac gtcgccaacg
     1201 acttcgcgcc cgcggaccaa ctgtatcgaa ataatggcga cggcacattc tccgacgtgc
     1261 tcgatcaagt cgtgccgcac acgccctatt cgtcgatggg ttccgacctc ggcgacataa
     1321 acaacgacgg cctcatcgac ttcttcgtcg ctgacatggc gacgacgacg cacgcaaagg
     1381 atctccgcac gatggcagag gttcgcgccc gcgcgcgcga tccccgcgac cgtgcccagg
     1441 cgccgcagta tacgcgggca gcactctatg tgaacaccgg cgtcggacgg ttgctcgagg
     1501 gagcgttgct cgccggcgtc gccgccaccg actgggcgtg gtcggttcga ttcgaggatc
     1561 tcgacaacga cggccgactc gatctgcacg tgaccaacgg aatgtttcgc gaaattcaca
     1621 atgt
//
LOCUS       DASPLT010000775         3184 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_132839, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000775 DASPLT010000000
VERSION     DASPLT010000775.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 3184)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3184)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3184
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_132839"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1283..1582
                     /locus_tag="VEA63_08040"
     CDS             1283..1582
                     /locus_tag="VEA63_08040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD83989.1"
                     /translation="MAGLTCPSTFAKTIIASSTVTDSPSRILNPQGEVVADANGDFAH
                     AIAEVDLNQQWRLRYMSVGNGEGEPASLMIKERRPDAYAPLLDTPVSMPARSPAK"
     gene            1900..>3184
                     /locus_tag="VEA63_08045"
     CDS             1900..>3184
                     /locus_tag="VEA63_08045"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HYD83990.1"
                     /translation="MVRGESENGLVLVSIHEDLLPGIRAVLPEFERRTGVPIAAQSVP
                     YSAHELWIRTQMLSRKAPDVLLVESTDLPWRYGQAGLLAAWDEWLQSPNPHDAGHLAR
                     PWQEAFHNELIHQARDAAGTIWCVPYTQYGVGFFYNRGVYEELRLTSPQTWEELLANF
                     GAVRADGRSALVTAVKPNDHQSLWMADMLLELLLRPVAEQVNLQTRAGWRYDPLDPEC
                     TRGEVITLEERLVAFDRGLIDPARAPAFRETARLMRRLAAEFRPDFLSLDGEEVPRVF
                     ARGESVHFLNGTWFLRPLTTMQRSIADIAPDRVFPWGVFPFPELTAETTALPRLGGIN
                     QNSGLRACFIVPRQPANPERERRAIDLVRFLTAPAVAEQAFGETAVYDLPAMTDVPPK
                     PGAEPLVPKVRYAFLVLAQFRGYDARSEGEFWTLWQ"
BASE COUNT          548 a          919 c         1077 g          640 t
ORIGIN      
        1 ggcggcgtcg gcgctggccg cgtgctcaac gcgcgtgacc caaagtctgg gtttggcgta
       61 gtggaacgtc gggggaacga gaggtgatgc ttcacttttt agcaaacgga gattcttcat
      121 tcaccggagc atcagaagcc gctcgcctac gccgtggcac tctagtcgcc cgcacgtatt
      181 tcgggggcct agcgagttct cccagcagcc tccgccagat cgtcaacgcg atctttctgg
      241 gcagagatga gttcatccgc gaccgtgtgg ggcaacggcc cacataagcc tccgtgcctc
      301 ccgttgcgtt ctagtttgag ccaccaaaga gccactaaat gtccgggaaa tttgtaaatg
      361 gctcgcaatc gggctggttg aaccgggttc gagtcccgtc catcccgcca ttttgaaaac
      421 ccgttaaccc ttaaaagtta gcgggttttc tgtttcaaaa agtgtcgtta gtggcaacga
      481 gagtggcaac acggccaccc acagcgccct gaatctccta acttcgggtc cacggcccta
      541 tcgtctggtg aagtttgtcg gcgagcagcg cgacaaagcg cgccgagagg agccggcgat
      601 gaggctcctt ttgctgcggt ttgacgccgc aattcgtgtc gcgcgcgccc cgttttactc
      661 gcgagtgccg cgtgccgtgg tgtccctgcg caacgagcgg gagggccggc ggcggcgaag
      721 aacaagcgaa ggcgggtttc ctttagggtg ccttcgtttg ttctggtcgc gcagcgacga
      781 cgccgatggc ggggcccgcc agcgggacac cacggttcgc gtcgctagtg ctggatggcg
      841 cgccttcacg tgcccgtcat ccttatgaac tcataagggt tttgcgtctt ctttagaaaa
      901 aaagaaaacg ggggagcgac cgcggctgcg gctctccatc gcggtgaggc cgacaaacca
      961 cggcgcgata ccggcgacgt ggtcgcgagg gaatggaaga ctcccccgcc tatactaaaa
     1021 tttaagcaga ccacatactg cgatctcagc aggggcgcgg tgttgcgggt ggcgacgaga
     1081 gtcgtggatt tcttggcgtg tgatggattc agtttatttt tcgttcattc gggcacgcgc
     1141 acgccgctgc actgcgggta gtcccgccga tgcgggagta gccggacgag ccgctacctc
     1201 aaactcggta gccgcgcgcg attgtcaggg acgtggtctc ggagaaacgg tgaaatcatg
     1261 atcacggtcg gcgtcattcg taatggcggg acttacctgt cccagcacct tcgcgaaaac
     1321 gattattgcg tcgtcgacgg tcacggactc gccgagtcgc attctgaatc cgcaaggcga
     1381 agtggtggcc gacgcgaacg gcgattttgc gcatgcgatc gcggaggtgg atctcaacca
     1441 gcaatggcgg ttgcgctaca tgtccgtcgg caatggcgag ggcgaaccgg cctcgctcat
     1501 gatcaaggag cgccggcccg atgcgtatgc gccgttgctc gatacgcccg tgtcgatgcc
     1561 ggcgcgttca ccggcgaagt gacatcgcct ccgaccacgc cgctcggttc gtgcgagcgt
     1621 cggtgggaag agcgaaactc gcgcgaagag cgttgacagt ttagcgccgt cgccatgatg
     1681 gccctaggcc gacatatgtc ggtcgatcac ccctcggaca cgttgaagga cctcttgcaa
     1741 cttagctctc attcgatgtt tcggcatgtc agtttccagt tcacggctcg ttcggcgagg
     1801 ggcctgaggg tgaccgaaaa aagccgccgc ttcggctcgg gtgcgcggcg aggtcgtttc
     1861 ttcgcggggg ttgggatcgc gtgggcgctt ctcacggccg tggtgagggg cgaaagcgag
     1921 aacgggttgg tgctggtgtc gattcacgag gatctgctgc ccgggattcg cgcggtgctg
     1981 ccggagtttg aacggcgaac gggcgtgccg atcgcggcgc agtcggtgcc atactcggcg
     2041 cacgagctgt ggattcgcac gcagatgctg agccgcaaag cgcctgatgt gctgttggtc
     2101 gagagcacgg atttgccgtg gcgttacggg caagccgggc tgctggccgc gtgggacgag
     2161 tggctgcagt cgccgaatcc gcacgatgcc ggtcatcttg cgcgaccgtg gcaggaggcg
     2221 tttcacaacg agctgattca tcaggcgcgt gacgcggcgg ggacgatttg gtgcgtgccc
     2281 tacacgcagt atggtgtcgg gtttttctac aatcgcggcg tttatgagga gcttcgttta
     2341 acgtcgccgc aaacgtggga agaactgctg gccaacttcg gcgccgttcg cgccgatggt
     2401 cgcagcgcgc tcgtcacggc ggtgaagccg aacgatcacc agagtctttg gatggccgac
     2461 atgctgctcg aactgctgct gcggccggtg gcggaacagg tgaatctgca aacgcgcgcc
     2521 ggctggcgct acgatccgct cgacccggag tgcacgcgcg gcgaggtgat cacgctcgag
     2581 gaacgattgg tggcgttcga tcgcggattg atcgatccgg cgcgcgcgcc cgcgtttcgc
     2641 gagacggcgc gtttgatgcg ccggctcgcg gcggagtttc ggccggattt tttgagcctc
     2701 gatggcgaag aggtgccgcg tgtgttcgcg cgcggcgagt cggtgcattt tctcaacggc
     2761 acgtggttcc tgcgtccgct cacgacgatg cagcggagca tcgccgacat tgcgccggac
     2821 cgcgtgtttc cgtggggcgt gtttccgttt cccgaattga cggcggaaac caccgcgctg
     2881 ccacgactcg gcgggatcaa tcagaactca gggctgcgcg cctgcttcat cgtgccgcgt
     2941 cagccggcaa atccggagcg cgagcggcgc gccatcgacc tcgtgcgttt tctcaccgcc
     3001 ccggcggtgg cggagcaagc attcggcgag acggcggtgt atgatttgcc ggcgatgacc
     3061 gacgtgccgc cgaagccggg cgccgagccg ctggtgccga aggtgcgcta cgcgtttctc
     3121 gtgctcgcgc agtttcgcgg ctacgatgcg cgcagcgagg gcgagttctg gacgttgtgg
     3181 caaa
//
LOCUS       DASPLT010000776         2165 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_33315, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000776 DASPLT010000000
VERSION     DASPLT010000776.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 2165)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2165)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2165
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_33315"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(350..1450)
                     /gene="hisC"
                     /locus_tag="VEA63_08050"
     CDS             complement(350..1450)
                     /gene="hisC"
                     /locus_tag="VEA63_08050"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HYD83991.1"
                     /translation="MREGMNFQSLVKPAVLTQPVYEPGKPIETVARELGLDPATIIKL
                     ASNENPFGPSPRAVAAAKAALEHAELYPDGGCFALREKLAAARGLKADQFVIGNGSNE
                     IIELLGHALLGPGDEVVMGAPAFVVYKLVTLLFGAKAVEVPLVNHRHDLAAMAAAITP
                     RTKLVFVCSPNNPTGTANTEVELRAFARALPEHVVVAFDEAYAEYVENAPDLRPLLSE
                     GRHVVCLRTFSKIYGLASLRVGYGYAAAETIAVLNRVRQPFNANAIAQAAACAALDDG
                     EFAEKCARENRAGMALLEAGFQKLGLEFVPSVANFVLVRVGDGARMFEALQRKGVIVR
                     PLKPYGMPEWVRVTVGTQTQNERLLLEMAKGL"
     gene            complement(1530..>2165)
                     /locus_tag="VEA63_08055"
     CDS             complement(1530..>2165)
                     /locus_tag="VEA63_08055"
                     /inference="COORDINATES: protein motif:HMM:TIGR02009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase family hydrolase"
                     /protein_id="HYD83992.1"
                     /translation="AGGFMKLDIPNGDFAGYIFDLDGTLIDTMPLHYRAWDAAMRKAG
                     LTVQLDEELFYSLGGVPTRKVAELIAKHYGLTIEIDRVFEDKESLFTELQKDARLIAP
                     TVAFAREAAKTHPLAVASGGPRDIVRRSLELAGLLPLFKAVVSADDVVHGKPAPDMFL
                     LAAKLIGVEPTRCLVFEDAEPGIKAALAAGMQVVRVPSRGAAAKRNVPVSG"
BASE COUNT          437 a          790 c          555 g          383 t
ORIGIN      
        1 gcattgtcgt cgaaagcgag aaacgccgaa gcgggagaga ccgccgcgca tagcgcggac
       61 gcggtcaaca aataggtaaa cgtcttcatc tcggtgtgat caaagttgca gtggaagctt
      121 cacgccggga aagccccttc agccgcataa gacttcaccg aattaaaccg gaaggagtgc
      181 tacacttttg aagacggact gccctggcga tgagcggacg ctttttaaat cagcacgcgc
      241 catgccgagg caagtgcgaa attaggtaat tttccccggt atccgaccgg gattcttcct
      301 cgggggcgta aacactcagc gtgcactcgt ctctcgcctc acatgctcgt cacaatccct
      361 tcgccatctc taggagaaga cgctcgttct gggtttgggt gcccaccgtt acgcgcaccc
      421 attccggcat tccgtatggc ttcagcggcc gaacgatcac ccctttccgc tgcaacgctt
      481 caaacatccg agcgccatcg ccaacacgca ccaagacgaa attcgccacg ctcggcacga
      541 actcgaggcc gagcttctga aatcccgctt ccagcagagc catccccgcg cggttctccc
      601 gcgcgcactt ttcggcgaac tccccgtcgt cgagcgcggc gcatgccgcc gcttgggcga
      661 tcgcgttcgc gttgaacggc tgccgaacgc gattcagcac cgcgatcgtc tctgcggcgg
      721 cgtaaccgta accgactcgc agcgacgcca acccgtaaat cttcgaaaac gtccgcaggc
      781 acaccacgtg tcgcccttcg ctcaacagcg gtcgcaggtc gggcgcgttt tccacatatt
      841 ccgcataagc ctcatcgaac gccaccacga catgctccgg caacgcacgg gcaaacgccc
      901 gcaactccac ctcggtgttc gccgtccccg tcggattgtt gggactgcac acaaacacca
      961 gcttcgttcg tggcgtgatc gccgccgcca tggcggccag atcgtggcgg tggttcacca
     1021 acggcacttc caccgccttc gccccgaaca gcagcgtcac cagcttataa accacaaaag
     1081 ccggcgcacc catcacgacc tcgtcaccgg ggcccaacaa cgcgtgcccc aacaactcga
     1141 tgatctcatt cgaaccattg ccgatcacga attgatccgc cttcaacccg cgcgcggccg
     1201 ccagtttctc ccgcagggca aaacacccgc cgtccggata caactccgcg tgctcgagcg
     1261 ccgccttcgc cgccgccacc gcccggggcg aaggcccgaa cgggttttcg ttcgacgcca
     1321 acttgatgat cgtcgccgga tcgagcccaa gctcgcgcgc caccgtctcg attggtttgc
     1381 cgggttcata caccggctgc gtcaacaccg cgggcttcac caacgactga aaattcatgc
     1441 cttcacgcat ggacgcgatt cgtcgcgaat gaaagttcgt ttcgctcagc gccacgttcg
     1501 cggcaccact caggcgcctg ctttttccgc tacccgctga ccggcacgtt tcgctttgcc
     1561 gccgcccccc gactcggcac gcgcacgacc tgcatgccgg ccgccagcgc cgccttgatg
     1621 cccggctccg catcctcgaa cacgaggcag cgcgtcggct ccacaccgat caacttcgcc
     1681 gccaagagaa acatatccgg cgccggtttc ccgtgcacca catcgtcggc gctcaccacc
     1741 gccttgaaca gcggcagcaa acccgccagc tccagcgaac gccgcacaat gtcgcgcggg
     1801 ccgcccgatg ccaccgccag cggatgcgtc ttcgccgcct cgcgcgcaaa cgccaccgtc
     1861 ggcgcgatca accgcgcatc cttctgcaat tcggtaaaca gcgactcctt gtcctcgaaa
     1921 acgcgatcga tctcgatcgt cagcccgtag tgcttcgcga tcaactccgc gacctttcgc
     1981 gtcggcaccc cgcccaacga ataaaacagc tcctcgtcga gctgcacggt caggcccgcc
     2041 ttccgcatcg cggcatccca agcgcgatag tgcaacggca tcgtgtcgat gagcgttcca
     2101 tcgagatcga aaatataccc ggcgaaatcg ccgttcggaa tatccagttt catgaacccg
     2161 ccagc
//
LOCUS       DASPLT010000777         1530 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_213752, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000777 DASPLT010000000
VERSION     DASPLT010000777.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1530)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1530)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1530
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_213752"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1380
                     /locus_tag="VEA63_08060"
     CDS             <1..1380
                     /locus_tag="VEA63_08060"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HYD83993.1"
                     /translation="GVAAWGFIRFLALRSAVEIPLLQSVRLDAWSFGFTVLLCLIAVV
                     LCGAMPAWRLAENDDLQHSLKDDARGAAGGRQRTRTRGTLVVLEVALACVLTLSAGLM
                     VRSFFNLLKVDLGFEPRDLIAMRIDPMIEGSHANYLESVLERVRALPGVEHAAVTDCV
                     PVERDRTWGLYPVIQDHPNDQRWTGAHVRIVSPGSFTAMGTRLIAGRDFTHMDTGDAP
                     PVIIINESLAKLFWPGENPLGRQVSGGNKVRTVIGVVADVRHSGPELPSGNEMYFSFR
                     DVGSNSWDMLVRTKLPVSTLTADLRNALRDVDATLPLTKVRTLQSLVDRTLSSRRLLV
                     WLIGGFAAIAVGLAALGLYGLISYTVTQQTKEIGIRMALGADASTVQWHVVGQTMKLA
                     GLGLALGLGVAFAAGRWLQSLLYGVSANDAVNALVTATTLIGCALIAGYLPARRASRV
                     DPLVALRAD"
BASE COUNT          237 a          424 c          552 g          317 t
ORIGIN      
        1 ggagtcgcgg cgtggggttt tattcgcttt ctcgcgctgc gctcggccgt ggaaattcca
       61 ctcctgcaaa gtgtgcgact cgatgcgtgg tcgtttggat tcacggtgtt gctctgcctg
      121 atcgcggttg tgctgtgtgg cgcgatgccg gcgtggcggc tggcggaaaa cgacgatctg
      181 cagcattcgc tgaaggacga cgcgcgcgga gcggcgggcg gacggcagcg cacgcgaacg
      241 cgcggcacgc tggtcgttct cgaggtggcg ctcgcgtgcg tgctcacatt gtcggccggg
      301 ttgatggtgc gcagtttttt caacctgttg aaagtcgatc tcggctttga gccgcgagac
      361 ctgatcgcga tgcgcatcga cccgatgatc gaaggatcgc atgcgaacta tctcgaaagc
      421 gtgctcgagc gcgtgcgggc gctgccgggc gtcgagcacg cggcggtgac cgactgcgtg
      481 ccggtggaac gcgaccgcac gtggggactt tatccggtga ttcaggatca tcccaacgac
      541 cagcgttgga cgggcgcgca cgtgcgcatc gtgagccccg gatcgttcac tgcgatgggc
      601 acgaggctca tcgcggggcg cgacttcacg cacatggaca ccggcgatgc gccgccggta
      661 atcatcatca acgaaagcct cgcgaaactt ttctggcccg gcgaaaatcc gctgggccgt
      721 caggtcagcg gcggaaacaa ggttcgcacg gttatcggcg tcgtggcgga tgtgcgacat
      781 agcggaccgg agctgccctc gggcaacgaa atgtattttt cgtttcgcga cgtgggatcg
      841 aactcgtggg acatgctggt gcggacgaaa ctgccggtgt cgacgttgac ggctgatctg
      901 cgcaatgcct tgagggacgt cgacgcgacg ctgccgctga cgaaggtgcg cacgttgcag
      961 agtttggtcg accgcacgtt gtcttcgcgg cggctgctgg tgtggctgat tggcggtttt
     1021 gccgcgattg cggtcggttt ggcggcgctc ggactttacg ggctgatttc ctacacggtc
     1081 acgcagcaga cgaaggagat cgggattcgc atggcgctgg gtgctgacgc ttcgacggtg
     1141 caatggcacg tcgttggcca gacgatgaaa ctcgcggggt tgggcctggc gctcggtttg
     1201 ggcgtggcat tcgcggccgg ccgctggttg caatcgctgc tctacggtgt gtccgcgaac
     1261 gacgccgtga atgcgctggt gactgcgacg acgttgatcg gctgcgcgtt gatcgcgggt
     1321 tatctgccgg cgcgccgggc ttcgcgcgtc gatccgttgg tcgcgctccg cgcggattaa
     1381 tgccagcggc gcgtgaccgc agcagcgcgg ggaatgggga accgtcgaga cggaatttgc
     1441 ctacgcacgg gtccggcgat ctgccgatgg cgggccgaca ggacggcggc tatgatgtgc
     1501 gatgggttac attcttcttt tgattctcac
//
LOCUS       DASPLT010000778         1096 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_111133, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000778 DASPLT010000000
VERSION     DASPLT010000778.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1096)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1096)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1096
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_111133"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(194..703)
                     /locus_tag="VEA63_08065"
     CDS             complement(194..703)
                     /locus_tag="VEA63_08065"
                     /inference="COORDINATES: protein motif:HMM:NF025070.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheX"
                     /protein_id="HYD83994.1"
                     /translation="MTAVKSPVTDQVIQECIIRSVQTVFHTMAGDNVTFVEQATEESG
                     SLPLAASHIIASVGFLGAADGLIYLCLPEEFAKIASGRILGMSRQEVEMHGEEVVHDA
                     IGEITNMTVGGFKNTLCDMGFPCKLTLPAIVRGNKLSIAAIKSATRHIFHFECSGHRL
                     TVDIQLKID"
     gene            complement(700..>1096)
                     /locus_tag="VEA63_08070"
     CDS             complement(700..>1096)
                     /locus_tag="VEA63_08070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD83995.1"
                     /translation="SSSMIRSMARRSRRTLWLEHFIPMMRDAEFPSPRCPFHRSFRSP
                     NSSKNSPVPHRARRITVEEANRTIFSARPSPPLRMPIWFLREIHERFVPLATDTPTRF
                     SSKSKPLPFLPKTCLQETRFGPNYYANLP"
BASE COUNT          246 a          269 c          373 g          208 t
ORIGIN      
        1 gaccgcgaaa ggcgaagcgc tggtcgagcg gctatcggcg gcgcactggg aggagttgcg
       61 gcaaatccgt ccggagttgc agcgactgct ggactcgatg gacgaccagt gagcggaccg
      121 aagcgaaggt aaaaaaacaa agccgcgcag cggtgaggct gcgcggcgcg gaggatccgg
      181 gactggacct ggatcaatcg atcttgagtt ggatgtccac cgtcagccgg tggccggagc
      241 actcgaaatg gaaaatgtga cgcgtggcgg atttgatcgc cgcgatggag agcttgttgc
      301 cgcgcacgat ggcgggcagc gtcagtttgc aggggaaacc catgtcgcag agcgtgtttt
      361 taaaaccgcc gacggtcatg ttggtgattt cgccgatggc gtcgtgaacg acttcctcgc
      421 cgtgcatctc gacttcctgg cgcgacatgc cgaggatgcg gccgctggcg atcttcgcga
      481 attcctcggg gaggcagaga tagataagac cgtcggcggc gccgagaaag ccgacgcttg
      541 cgatgatgtg cgaagcggcg agcggcagcg aaccggactc ctccgtcgct tgttcgacga
      601 acgtaacatt gtcgccggcc atcgtgtgaa acacggtttg gacggaccga atgatgcatt
      661 cttggatcac ttggtcggtg acaggagatt tgacggccgt catggtaagt tcgcgtaata
      721 gttcgggccg aaacgggttt cctgaagaca agtttttggc aaaaatggca atggtttgga
      781 tttagacgaa aagcgcgtgg gagtgtccgt cgccaaaggc acgaagcgct catggatttc
      841 ccgaagaaac cagatgggca tgcggagcgg cggtgaaggg cgtgcggaaa aaatcgtcct
      901 atttgcttcc tccacagtga tgcgccgagc gcgatgcggc acaggagagt ttttcgatga
      961 attcggactg cggaaggacc gatgaaatgg gcagcgcggc gaggggaatt ccgcatcgcg
     1021 catcatcgga atgaaatgtt cgagccacag cgttcgtcgc gaccttctag ccatcgagcg
     1081 aatcatcgac gaggaa
//
LOCUS       DASPLT010000779         2345 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_234583, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000779 DASPLT010000000
VERSION     DASPLT010000779.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 2345)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2345)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2345
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_234583"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..180)
                     /locus_tag="VEA63_08075"
     CDS             complement(<1..180)
                     /locus_tag="VEA63_08075"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HYD83996.1"
                     /translation="MRRFSYLPNLLSSLRIALAPAMLGAAYSNAKIGFAVLLAAVLVT
                     DVVDGFLARRWRAVSE"
     gene            complement(199..645)
                     /locus_tag="VEA63_08080"
     CDS             complement(199..645)
                     /locus_tag="VEA63_08080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009509689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HYD83997.1"
                     /translation="MRIAVISDTHDRYPNGLPGRLKGADEVWHLGDVCDPLTLVEFEA
                     LRVPLHVVMGNCDEHAWPMSLTLERGGLRFFLTHIPPERARVAPGVSAVLHGHTHVPR
                     DETIGGVRWLNPGCITRPNRGAAASFAWLTVEGGRMRWEPVRVKPD"
     gene            945..1082
                     /gene="rpmH"
                     /locus_tag="VEA63_08085"
     CDS             945..1082
                     /gene="rpmH"
                     /locus_tag="VEA63_08085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HYD83998.1"
                     /translation="MKPTFRPHRKKRARKIGYRARMATPGGRKVIKARRLKGRKRLTV
                     V"
     gene            1172..1552
                     /gene="rnpA"
                     /locus_tag="VEA63_08090"
     CDS             1172..1552
                     /gene="rnpA"
                     /locus_tag="VEA63_08090"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007363088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HYD83999.1"
                     /translation="MRFRPEQHLRRQSDFRAVREHGRRYNCGPFTLWCLRRDPATASR
                     PVAPADLRRVGVVASTASVGHAVLRNRAKRRLREIFRRHQESVPAGCDLLLTARAAMV
                     DAPLPELEKKFVEACQRVAPREKQ"
     gene            1549..1989
                     /gene="yidD"
                     /locus_tag="VEA63_08095"
     CDS             1549..1989
                     /gene="yidD"
                     /locus_tag="VEA63_08095"
                     /inference="COORDINATES: protein motif:HMM:NF013932.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HYD84000.1"
                     /translation="MNHSSVMLPSSTTPISHRTAMTARASSAADHTQPFQTAASLLLS
                     SLRFIVALPARVLLAAIWLYQRTLSPALPVVLGPSFGCRFAPTCSHYSAQALRTHGAI
                     VGTWLTLIRLLKCTPLHPGGFDPVPRRAAPRCTRIAPPPLASRS"
     gene            2005..>2345
                     /locus_tag="VEA63_08100"
     CDS             2005..>2345
                     /locus_tag="VEA63_08100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD84001.1"
                     /translation="MDKKNTTIGVLLLIAAFASMYLGNRLSPNRSAEPVPSLNQPAVA
                     DAPAGAPVTGGAPQSLAPAANGATSTLSSTTAFASAVSDTASATVTTLENEFIAAQFT
                     NSGGAVRNVAF"
BASE COUNT          408 a          929 c          582 g          426 t
ORIGIN      
        1 ttcggaaacc gcgcgccacc tcctcgcgag aaaaccatcc accacgtccg tcaccaacac
       61 cgccgccagc agcaccgcga aaccgatttt tgcattcgaa tacgccgccc ccaacatcgc
      121 cggcgccagc gcgatccgca ggctgctcaa cagattcggc aaataagaga agcgtcgcac
      181 agcgatcaga ggcccgcgct agtcaggttt aacccgcacc ggttcccacc gcatccgacc
      241 gccttccacc gtcagccacg cgaaactcgc cgccgccccg cgattcggcc gcgtgatgca
      301 gcccgggttc aaccagcgca cgccgccgat cgtctcgtcg cgcggcacat gcgtgtgacc
      361 gtgcagcact gcgctcactc ccggcgcgac ccgcgcccgc tccggcggaa tatgcgtgag
      421 aaaaaaacgc agcccgccgc gctccagcgt cagcgacatc ggccacgcat gctcgtcgca
      481 attccccatc accacgtgca atggcacgcg cagtgcttcg aactccacca gcgtcaacgg
      541 atcgcacacg tcgcccaaat gccagacctc gtccgcgccc ttcaaccgac ccggcaaccc
      601 gttcggataa cgatcgtgcg tgtcggagat aacagcgatg cgcatggcga accgtaaacg
      661 cgccgccatc ctgatcgcta gccggaaacc ttccgcatca gatatacttc cagcgactcg
      721 ccgccccacg actcctcttc gtgcctccgt ggttcaaatc ccgtccgctc aatccgtgtt
      781 cttctgtgtt catccgtggt taaaccactg ccgcaatcga ttgtcgtcac tcgaaaattt
      841 cgctccatcg cccgacccat tccactgtaa ttcgctctcc aaatcccgcc cttgacactc
      901 cttgcgccgt cctgcaccac tgaccctctt tttcatctga agtcatgaaa cccacattcc
      961 gtccgcaccg caaaaaacgc gcccgcaaga tcggttaccg cgcacgtatg gccactcctg
     1021 gcggccgtaa agtcatcaaa gcccgccgcc tcaagggccg caagcgcctc acggtcgtct
     1081 gacctcgaca ggccggtgcg agtgacagtg aaagtaaaag tgccaagccg ctgccgctct
     1141 cactttcgct ttcccggtca ctctcacttc gatgcgtttc cgccctgagc agcatttgcg
     1201 gcgtcagagc gatttccgcg cggtccgcga acacggccgc cgttacaact gcggcccttt
     1261 caccttgtgg tgcctgcgcc gcgatcccgc caccgcgagc cgtccggttg cacccgctga
     1321 tctccgccgc gtcggcgtcg tcgcctccac cgcttccgtc ggccacgccg tccttcgcaa
     1381 ccgcgccaaa cgccggcttc gcgaaatctt tcgccggcat caagagagtg ttcctgccgg
     1441 ctgcgacctt cttctgaccg cgcgcgccgc gatggtcgat gcgcccctcc cggaactcga
     1501 gaaaaagttc gtcgaggcgt gtcaacgggt tgcgccgcgc gaaaagcaat gaatcactcg
     1561 tcggtcatgc tgccctccag caccacgccg atctcccacc gcaccgcgat gaccgcgagg
     1621 gcgtcgtctg ccgccgacca cactcaaccg tttcaaaccg ccgcctcgct cctgttgtcc
     1681 tctttgcgct tcatcgttgc cctgcctgcg cgggtcttac tcgccgcgat ctggctctac
     1741 caacgcacgc tctcgccagc gctgcccgtc gtcctcggcc cgtctttcgg ttgtcgtttc
     1801 gcccccacct gttcgcacta cagcgcgcaa gccttgcgca cccacggcgc gatcgtcggc
     1861 acctggctca cgctgatccg cttgctgaaa tgcacccccc tccaccccgg cggtttcgat
     1921 cccgtcccgc gccgcgctgc accgcgctgc acgcgcatcg ctccgcctcc cctcgcgtcc
     1981 cgttcctagc ccagctcctt tttcatggat aagaagaaca ccaccattgg cgtgctgctg
     2041 ctcatcgcgg cgttcgccag catgtatctc ggcaatcgcc tttcgcccaa ccggtccgcc
     2101 gaaccggtgc ccagtctcaa ccaacctgcc gtcgccgacg ctcccgctgg agctcccgtg
     2161 acgggcggcg caccgcaaag cctcgcgccc gcggccaacg gcgccacgtc cacgctctcg
     2221 tccacgactg cgttcgcgag cgccgtcagc gacaccgcgt cggccaccgt tacgactctc
     2281 gagaacgagt tcatcgccgc acagttcacg aactccggcg gcgccgtccg caacgtcgcg
     2341 ttcaa
//
LOCUS       DASPLT010000780         1539 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_111210, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000780 DASPLT010000000
VERSION     DASPLT010000780.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1539)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1539)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1539
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_111210"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..807
                     /locus_tag="VEA63_08105"
     CDS             <1..807
                     /locus_tag="VEA63_08105"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator
                     substrate-binding protein"
                     /protein_id="HYD84002.1"
                     /translation="GHKLFHRLGRKAVLTEAGAVFLERARRILFEVENASRELSDHPS
                     LDRRITVGALPTVAPYLLVPLIARCRKELPNLTIHVREDFRANLVRAVAEGDLDLAVV
                     SLPIKDPRLSVEPLMTEPLLLIVGKNHEFVRRSEINVNDLAEETFVTMGDSSTLAAQI
                     QAFFGDHNFSPKIGYRCAQVATLKAFVAMGAGISILPQVDRMPEDRDTLTYLRLAGSA
                     PTRELGVIRHLQRYQSRGAEQFLSVLRAHAQAKQFEAFPPDDESPPAPSG"
     gene            905..>1539
                     /locus_tag="VEA63_08110"
     CDS             905..>1539
                     /locus_tag="VEA63_08110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007362620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HYD84003.1"
                     /translation="MSSAPHEQPAAKERVILLTLAAVQFTHVLDFMIMMPLGSHLMRV
                     FRIDPAQFSHLVAAYGIAAALSGFAAGFVLDRFDRKHALFALFSGFSLATLACALAPG
                     YGALLIARLAAGAFGGVASSVVVSMVGDVIPPARRGRAMGVVAVAFPIASVLGVPISL
                     VLAEKFEWHAPFFLLAAISAGVVVLIWRVLPSLRSQHAPAHPWQQMKSILG"
BASE COUNT          230 a          566 c          425 g          318 t
ORIGIN      
        1 ggccacaaac tcttccaccg gctcggccgc aaagccgtgc tcaccgaggc cggcgccgtt
       61 ttcctcgaac gggcgcgtcg catcctcttt gaagtcgaaa acgcttcccg cgaactcagc
      121 gatcacccct ccttggaccg ccgcatcacc gtgggagcgc tgccgacggt tgcgccctac
      181 ctgcttgttc cgctgatcgc ccgttgtcgg aaggagttac ccaatttgac catccacgtg
      241 cgcgaagact ttcgcgccaa tctcgttcgt gccgtggccg agggtgacct cgatctggcc
      301 gtcgtttccc tcccgatcaa ggatcctcgc ctctccgtcg agccgctgat gaccgaaccg
      361 ctgttgctca ttgtgggcaa aaaccacgag ttcgttcgtc gctccgagat caacgtcaac
      421 gatctcgccg aggaaacttt cgtcacgatg ggcgactctt ccacgctcgc cgctcagatt
      481 caggcgttct tcggcgacca caatttcagc ccgaaaatcg gctaccgctg cgctcaagtc
      541 gcgaccctca aagccttcgt cgccatgggc gccggcatct ccatccttcc gcaagtcgac
      601 cgcatgcccg aagaccgcga tacgctcacg tatctgcggc tcgccggcag cgcgcccacc
      661 cgcgaactcg gcgtcatccg ccaccttcaa cgttaccaga gccgcggcgc cgaacaattt
      721 ctcagcgttc tccgcgcgca cgcgcaagcc aagcagttcg aagcgtttcc gccggatgac
      781 gagtcgccgc cggcgccgtc cggctgatcg ctcccacgcg atcgagcctc ctgcgccaaa
      841 gttttggccg cgatcggcgg ctccggttgc gctttcaacc gtttcgccaa cgctctcgcc
      901 cttcatgtca tccgctccgc acgaacaacc ggccgccaag gagcgagtga tcctgctcac
      961 gctcgccgcc gtgcaattca cgcacgtgct ggatttcatg atcatgatgc cgctcggctc
     1021 gcatctcatg cgcgtctttc gcatcgatcc ggcccaattc agccacctcg tcgccgccta
     1081 cggcatcgcc gccgcgctca gcggtttcgc cgcgggcttc gtgctcgatc gtttcgaccg
     1141 caaacacgcg ctgttcgcgc ttttttccgg ctttagcctg gcaacgctgg cgtgcgccct
     1201 cgctcccggc tacggcgcgc tcctcatcgc gcgactcgcc gccggtgcgt tcggtggcgt
     1261 cgccagctcg gtggtcgtct caatggtcgg cgacgtcatt ccgcccgcgc gtcgcggtcg
     1321 cgccatgggc gtggtcgccg tcgcgtttcc catcgcgtcc gtcctcggcg tgccgatcag
     1381 cctcgtgctc gcggagaaat ttgaatggca cgcgccgttt ttcctgctgg cggcgatcag
     1441 cgcgggcgtc gttgtgttga tctggcgcgt attgccttcg cttcggtcgc aacacgcgcc
     1501 cgcacatccc tggcagcaaa tgaagtcgat cctcggcga
//
LOCUS       DASPLT010000781         1720 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_152919, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000781 DASPLT010000000
VERSION     DASPLT010000781.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1720)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1720)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1720
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_152919"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..547
                     /locus_tag="VEA63_08115"
     CDS             <1..547
                     /locus_tag="VEA63_08115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD84004.1"
                     /translation="VTPPTSAPLVDPAAPSPVIVAPVIPNSAAAPEIPPAIEPVATST
                     TPPAIVVPDLAATTPASAPEVAPVATAPEPASSTAATSTPASAPAASEPPPTAAAAKL
                     AAAVEAIAPDSDVRAQQFIDALRVTGIRASGNDSKVLMNDRVYRVNDIVDRTLGLKLI
                     KVAPDSLTFSDPTGATYTKIF"
     gene            731..>1720
                     /locus_tag="VEA63_08120"
     CDS             731..>1720
                     /locus_tag="VEA63_08120"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HYD84005.1"
                     /translation="MLRFTLLTVLLSFPLLAQGAAEKRPHPARNIVFILTDDHRFDAM
                     GFMGHPFLETPNLDRLAREGAHLANAFVTTALCSPSRASILTGLYAHRHGVVDNNNPV
                     RADLVFFPQILQRAGYTTGFFGKWHMGGESAAPQRGFDKWVSFRGQGEYWPDRRGPGE
                     APRTTPQPYGPVPLNVDGHEVPQRGYITDELTEYALDWIRSVPKEKPFFVFLSHKAVH
                     ANFVPAERHAGRYHDKKLPTPLSRVQHEHAPMWVQNQRNSWHGTDFPYHTHLDIERYY
                     QRYCETLLAVDDSVGRVLDELRARGQLDDTLVVYMGDNAVVTHVNDERVVELTA"
BASE COUNT          304 a          655 c          472 g          289 t
ORIGIN      
        1 cgtcacgccc ccgacatcag ctccgctcgt cgatcccgcg gccccaagcc ccgtcatcgt
       61 cgcgccggtc attcccaatt ccgccgccgc acccgaaatc ccccccgcga tcgaacccgt
      121 cgccacaagc accacgccgc ccgccattgt tgttcccgat ctcgccgcga caacgccagc
      181 ttccgcgccc gaggtcgctc cggtcgcaac cgcacccgag cctgcgtcat cgaccgccgc
      241 cacttccacc cccgcctcgg cgcccgccgc ttcggagccg ccgccgaccg ccgccgccgc
      301 caaacttgcc gctgccgtcg aggccatcgc gcccgattcc gacgttcgcg cgcagcaatt
      361 tatcgatgcg ttgcgcgtca ccgggattcg cgcctccggc aacgacagca aagtcttgat
      421 gaacgatcgc gtttaccgcg taaacgacat cgtcgaccgc accctcggct tgaagcttat
      481 caaagtcgca cccgactcgc tcaccttctc ggacccaacg ggcgccactt acaccaagat
      541 tttctagccg cgtcggtagg gagcgccgag gcgtccgcgc aaatggaagc ggaagcgtcg
      601 cgcatcaccc ggagccgacc caacgaagct tgcacgctgc cgcgtcgcgt taaaaattcg
      661 gcgtcccacg accggctgcg cgccgcgtca gtcccccgga aagccgatgc caccgttttc
      721 tcaaatccgc atgctgcgct tcactctgct cacggttctt ctgtcgtttc cgctcttggc
      781 gcagggcgcc gcagagaagc gtccgcaccc ggcgcgcaac attgttttca tcctcaccga
      841 cgaccatcgc ttcgacgcga tggggttcat gggccatccg tttctcgaaa cgcccaacct
      901 cgatcgtctc gcgcgcgaag gcgcgcatct ggccaacgcc tttgtcacca cggcgctgtg
      961 ctcgccgagc cgcgcctcga ttctcaccgg cctctacgcg caccgccacg gcgtggtcga
     1021 caacaacaat cccgtccgcg cagacctcgt gttttttccg caaatcctcc agcgcgccgg
     1081 ctacacgacg ggatttttcg gcaagtggca catgggcggc gagagcgcgg ccccgcagcg
     1141 cggcttcgac aaatgggtga gctttcgcgg acagggcgaa tactggccgg atcgccgcgg
     1201 ccccggcgaa gcgccgcgca ccaccccgca gccgtatggt cccgtgccgc tcaacgtcga
     1261 cgggcacgag gtgccgcaac gcggctacat caccgacgag ctgaccgagt atgcgctcga
     1321 ctggattcgc tcggtcccga aggaaaagcc gtttttcgtc tttctttcgc acaaggcggt
     1381 gcacgcgaac ttcgttcccg ccgagcgcca cgctggacgc taccacgaca aaaaactgcc
     1441 gacgccactc tcgcgcgtgc aacacgagca cgccccgatg tgggtgcaga accagcgcaa
     1501 ttcgtggcac ggaacggatt tcccgtatca cacccacctc gacatcgagc gctactacca
     1561 acgctactgc gagacgctgc tcgccgtgga cgacagcgtt ggccgcgtgc tcgatgagct
     1621 ccgcgcgcgc ggtcagctcg acgacacgct cgtcgtttac atgggtgaca acgccgttgt
     1681 cacccatgta aacgacgagc gtgtcgtcga gctgaccgcg
//
LOCUS       DASPLT010000782         1689 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_33412, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000782 DASPLT010000000
VERSION     DASPLT010000782.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1689)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1689)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1689
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_33412"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..575
                     /gene="mraY"
                     /locus_tag="VEA63_08125"
     CDS             <1..575
                     /gene="mraY"
                     /locus_tag="VEA63_08125"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HYD84006.1"
                     /translation="LVLIYLWIVGFSNAINFTDGLDGLAVGCTISVALVFGIMAYAAG
                     NIILSEYLLMSFVPGTGELTVICGALIGGCMAFLWYNSHPAEVFMGDTGSLALGGLIG
                     VMAFMIHQPFTLVIVGGVFVAELLSVVIQIGWFKYTKRRFGVGRRVFLMAPIHHHFQK
                     KGWPETKVVLRFWVLSLGCALAGLATLKLR"
     gene            680..>1689
                     /gene="murD"
                     /locus_tag="VEA63_08130"
     CDS             680..>1689
                     /gene="murD"
                     /locus_tag="VEA63_08130"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HYD84007.1"
                     /translation="MRKLTLTVPESFRPWLTRPVGILGGGVSGVGVTALLATIGAESV
                     IYDAKGTPFSAKAAKAHGLVVFSPGFAPEHEWLALARAAGCECLNELDFASAFWRGAI
                     IAVTGTNGKTTLTEFLTHALRATSHEAHATGNIGHSFAQLVAASDGGTTETIAVCEVS
                     SFQAETLKRFRADATLWTNFAEDHLERHPGLESYFGAKWNLVARTKPGAVFAGSSVQR
                     FAEKFNRPLPASAAIQTEGVAADARLHGTVFAEYPQRENFVLAAAWWRVAGLPEEALI
                     TAAQNFQLGRHRLARIGEHEGVTFWNDSKATNFHAVEAALARFEKPVVLIAGGKAKGG
                     DLA"
BASE COUNT          288 a          407 c          639 g          355 t
ORIGIN      
        1 tgttggtgct aatttatctg tggatcgtgg gattcagcaa cgcgattaac tttacggacg
       61 ggctcgatgg gctggcggtg ggctgcacga tttcggtggc gctggtgttc gggatcatgg
      121 cgtatgcggc gggcaacatc attttgtcgg aatatctgct aatgagcttc gtgccgggga
      181 cgggcgaact gacagtgatc tgcggcgcgt tgattggcgg gtgcatggcg tttctctggt
      241 ataactcgca tccggcggag gtgttcatgg gcgatacggg ttcgctcgcg ctgggtggtt
      301 tgatcggcgt catggctttc atgatacacc agccgttcac gttggtgatt gtcggcgggg
      361 tgtttgtggc ggaactgctg tcggtcgtga tccagatcgg ctggttcaaa tacacgaagc
      421 ggcggtttgg cgtcggccgg cgggttttcc tgatggcgcc gattcaccat cattttcaaa
      481 aaaagggctg gccggaaacg aaagtggtgc tccgtttctg ggtgttgtcg ctgggctgcg
      541 cgctggcggg gctggcgacg ttgaaactgc ggtgaacgga gcgaaggcga gacatgaacg
      601 ttgtgcgcaa attgagagaa attcacggac cgctcggaga gcggtcccta ccggcgctgc
      661 cgcgccaacg ggctgactga tgcgcaagct gacgctgaca gttcctgaat cgttccggcc
      721 gtggttgacg cggccggtgg gaattcttgg gggcggcgtg agcggcgtgg gtgtgacggc
      781 gttgctggcg acgatcggtg cggagagcgt gatctatgat gcgaagggca cgccgttttc
      841 ggcgaaggcg gcgaaggcgc acggactcgt ggtgttctcc cccgggtttg cgccggagca
      901 cgaatggctg gcgctcgcgc gggcggcggg ttgcgagtgc ttgaacgagc tggatttcgc
      961 atcggcgttc tggcggggcg cgatcatcgc ggtgaccggc acgaacggaa agacgacgct
     1021 gacggaattt ctcacgcatg cgttgcgcgc gacgagccac gaagctcacg cgacgggcaa
     1081 catcgggcat tcgtttgcgc aacttgtggc ggcgtcagac ggtggcacga cggagacgat
     1141 tgcggtgtgc gaagtgagtt cgtttcaggc ggagacgctg aagcggtttc gcgcggatgc
     1201 gacgctctgg acgaatttcg cggaggatca tctcgagcgc catcccgggc tggagagtta
     1261 tttcggagcg aagtggaatc tcgttgcgcg gacgaagccc ggcgcggtgt tcgcagggtc
     1321 gtcggtgcaa cgcttcgcgg agaagttcaa ccggccgctg ccggcatcgg cggcgattca
     1381 gaccgaagga gtggcggcgg acgcgcggct gcacggaacg gtttttgcgg aatatccgca
     1441 gcgggaaaat ttcgtgctgg cggccgcgtg gtggcgggtg gcgggtttgc cggaggaagc
     1501 gttgatcacg gcggcgcaga attttcagct tggaaggcat cgactcgcgc gaatcggcga
     1561 gcacgaaggc gtgacgtttt ggaacgattc gaaggcgacg aattttcatg cggtcgaagc
     1621 ggcgttggcg cgcttcgaaa agccggtcgt gttgatcgcg ggtggaaaag cgaagggcgg
     1681 cgatctcgc
//
LOCUS       DASPLT010000783         1890 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_275107, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000783 DASPLT010000000
VERSION     DASPLT010000783.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1890)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1890)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1890
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_275107"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..194)
                     /locus_tag="VEA63_08135"
     CDS             complement(<1..194)
                     /locus_tag="VEA63_08135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD84008.1"
                     /translation="MAAAVPVPVSNPFNTLLKRADLLLTGGLFLTVLLLIMPIPTVLL
                     DLFLALNIGLSLLVLLAIVY"
     gene            complement(198..1301)
                     /locus_tag="VEA63_08140"
     CDS             complement(198..1301)
                     /locus_tag="VEA63_08140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscU/YscU/HrcU family type III secretion system
                     export apparatus switch protein"
                     /protein_id="HYD84009.1"
                     /translation="MADTDQDQKTEQPTEKKLSEAMDRGQFARSQELSVLFLLAGFLG
                     VLGFTLKTASRDVADYAVGMFSRFASIPVERDTINIQLGEALLISGRALAPMLLACVA
                     AALVAGGIQSGFRMSPNAIRFKPEGLDPIAGFGRIFNKSAFVKAGVDLLKLVAIGTAL
                     YLGARGLMFDPLFSAPVEAAYLGQFLSNATIVFVSRLIFALGVVTAISYGYEKFKTGR
                     ELMMTKQEVKDEAKNAEGNAMAKGAMRRMARRLMQKQMLSAVPTADVVVTNPTHFAVA
                     LKYERGKDQAPVILAKGENRFAQRIKALAAEHGVPTVENKPVARLLFAMGKVGDTIPA
                     ELYQAVAGILAVVYRTHRYYFHRLKARRLESIA"
     gene            complement(1489..>1890)
                     /locus_tag="VEA63_08145"
     CDS             complement(1489..>1890)
                     /locus_tag="VEA63_08145"
                     /inference="COORDINATES: protein motif:HMM:NF013478.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HYD84010.1"
                     /translation="AALLSTFAIVMFFLFGGHLMMLSALARSFSLILTGNPVLGPAAG
                     DSMIRATSHMIELGVRMAAPFIAMNFLVTMSFSALGRVVPKMNPFIISFSVKLMAGFT
                     LLASAGALMARYLYEEFSETPVRMLQVITGN"
BASE COUNT          389 a          544 c          628 g          329 t
ORIGIN      
        1 atgtagacga tcgcgaggag aacgaggagc gacaggccga tgttcagcgc gaggaacagg
       61 tccaggagga ccgtcgggat cggcatgatc aacagcagga ccgtgaggaa cagtccgccg
      121 gtgaggagga gatccgcgcg cttcaggagc gtgttgaacg gattgctgac gggaactgga
      181 acagcggcgg ccatggctta ggcgatcgat tcgagccggc gggccttgag gcggtggaag
      241 tagtatcggt gagtccgata cacgacggcg aggatgccgg cgacggcttg gtaaagttcg
      301 gcgggaatcg tgtcgcccac cttgcccatg gcgaagagga ggcgcgcgac gggcttgttc
      361 tcgaccgtgg gcacgccgtg ttcggcggcg agggccttga tgcgctgcgc gaaacggttt
      421 tcgcctttcg cgagaatcac gggcgcctgg tccttgccgc gttcgtattt caatgcgacg
      481 gcgaagtgag tcggattcgt gacgacgacg tcggcggtcg gcacggcgga gagcatctgc
      541 ttttgcatca ggcggcgggc catgcggcgc atcgcgccct tcgccatcgc gttgccctcg
      601 gcgttcttcg cctcgtcctt gacctcctgc ttggtcatca tcagctcgcg gccggtcttg
      661 aacttctcgt agccgtagct gatcgcggtg acgaccccga gggcgaagat gaggcgggaa
      721 acgaaaacga tcgtggcgtt cgagagaaac tgcccgaggt aggcggcttc gacaggcgcg
      781 ctgaagagcg gatcgaacat caagccgcgc gcgccgagat agagcgcggt gccgatcgca
      841 acgagcttga gcagatcgac gccggctttg acgaaggcgg acttgttgaa aatacgaccg
      901 aagcccgcga tcggatcgag gccttcgggt ttgaatcgga tcgcgttcgg cgacatgcga
      961 aatccgcttt gaatgccgcc ggccaccaac gcggcggcga cacacgcgag aagcatcggg
     1021 gccagcgcgc gaccgctgat gagcagcgct tcaccgagct ggatgttgat cgtatcgcgc
     1081 tcgaccggaa tcgaagcgaa gcggctgaac atgccgaccg cgtaatcggc gacgtcgcgc
     1141 gacgccgttt tcaaggtgaa accgagcacg ccgagaaagc ccgcgaggag gaaaaggacg
     1201 gagagctcct gcgaccgcgc aaactggccg cgatccatcg cctcggagag tttcttctcg
     1261 gtcggctgct ctgtcttttg gtcctgatcg gtatccgcca tgggcacgac ggtttagcaa
     1321 cgccgatgcc aagggggttt aaggtgtttt tagtcgctgc tgcacaggcg attaaacttt
     1381 tatcttccgc cgccgacccc ggaaaattcc cctggggtga agtcccgtat tttccgcggc
     1441 aaattttgcc gttccggggt taacccggag ccgccgacga caccccggct agtttccggt
     1501 gatcacctgc agcatccgca ccggtgtctc gctaaactcc tcgtaaaggt agcgcgccat
     1561 cagtgcgccg gcagaagcca gcagggtgaa accggccatc agcttcacgg agaaactgat
     1621 gatgaaggga ttcattttcg gcaccacgcg gcccagtgcg gagaaggaca tcgtcaccag
     1681 gaagttcatc gcgatgaacg gcgcggccat gcgcacgcca agttcgatca tgtgcgaggt
     1741 cgcgcgaatc atcgaatcgc ccgccgcggg acccagcacg ggattgccgg tgaggatgag
     1801 cgagaagctg cgcgcgagcg ccgacagcat catcaaatgt ccgccgaaca gaaaaaacat
     1861 cacgatcgcg aacgtcgaca acagcgccgc
//
LOCUS       DASPLT010000784         1073 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_234697, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000784 DASPLT010000000
VERSION     DASPLT010000784.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1073)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1073)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1073
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_234697"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(130..>1073)
                     /locus_tag="VEA63_08150"
     CDS             complement(130..>1073)
                     /locus_tag="VEA63_08150"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0016018 - cyclosporin A binding [Evidence
                     IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HYD84011.1"
                     /translation="NFVGLAEGTLGPTPRQPFFNGLAFHRVVPGFVIQGGDPLGTGEG
                     GPGYEFPDEFVPSLRHDAAGILSMANAGPDTNGSQFFITLAPVNRLNYLHSVFGRVVR
                     GLDVLPQIQRGDAITSVTIRRIGAAAEAFRADEAAFAALLAKTPRATPPHFDDLDGVL
                     PVDPPRAKTFDYKLANLERASGVKLYARVFANFTPETPAQRPGNFAGSLAKKLHLPPD
                     AILAVYFADLHQWGLWIGDDRLADFMGRPGTVREFTRSGALHEAKQSLLDTATADAEA
                     AIAEAEQRVPAQPLTAAQKIKLHVDAVLDALIFKFER"
BASE COUNT          187 a          247 c          459 g          180 t
ORIGIN      
        1 gcgaggagcg gggatgaggc gatgaggagg gaggcgagga agcggagggg catgaaggag
       61 gcgggtggat ggagcggatg aagatgcggt caggttgacc gcgctacggg tggcgtgcgg
      121 aggggggcct taccgttcga atttgaagat caaggcgtcg aggacggcgt cgacgtggag
      181 ttttattttc tgagcggcgg tgaggggttg agcggggacg cgttgttcgg cttcggcgat
      241 ggcggcttcg gcgtcggcgg tcgcggtgtc gaggagcgat tgtttcgctt cgtgtaaggc
      301 gccggagcgg gtgaactcgc gcaccgtgcc ggggcggccc atgaaatcgg ccaggcggtc
      361 gtcgccgatc caaaggcccc actgatggag atcggcaaag tagacggcga gaatcgcgtc
      421 gggcgggaga tggagttttt tcgcgagcga gccggcgaag ttgccggggc gttgggcggg
      481 tgtttccgga gtgaagttcg cgaagacgcg cgcgtagagt ttcacgccgc tggcgcgttc
      541 gaggttggcg agtttgtagt cgaaggtttt cgcgcgaggc gggtcgacgg gaagcacgcc
      601 gtcgaggtcg tcgaaatgcg gcggtgtggc gcgcggagtt ttggcgagga gggcggcgaa
      661 cgcggcctca tcggcgcgga acgcttcggc ggcggcgccg atgcggcgga tggtgacgga
      721 ggtaatcgca tcaccgcgtt gaatttgcgg caggacatcg aggccgcgga ccactcgacc
      781 gaagacgctg tgaagatagt tgaggcggtt gacgggagcg agggtgatga agaactgcga
      841 accgttggta tcggggccgg cgttggccat cgagagaatg ccggcggcgt catggcgtag
      901 actgggaacg aactcgtcgg ggaactcgta gccggggccg ccttcgccgg tgccgagcgg
      961 atcgccgcct tggatcacga aaccggggac gacgcgatga aaggcgaggc cgttgaagaa
     1021 cggctggcgg ggcgtggggc cgagtgtgcc ttcggcgaga ccgacgaagt tgg
//
LOCUS       DASPLT010000785         2182 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_296656, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000785 DASPLT010000000
VERSION     DASPLT010000785.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 2182)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2182)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2182
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_296656"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..426
                     /locus_tag="VEA63_08155"
     CDS             <1..426
                     /locus_tag="VEA63_08155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009508083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HRDC domain-containing protein"
                     /protein_id="HYD84012.1"
                     /translation="GRELMRLGYVAVAEGEFATLELTTEGMTVLVKRTPITLTKPMDL
                     PKTKRVARREGDIACDEILFERLRGLRKQLADERRVPAYVIFGDTTLRAMARHYPATV
                     DSLEGIPGIGEKKRAEFGELFATEIADYLRTNSRQSFND"
     gene            complement(685..1692)
                     /locus_tag="VEA63_08160"
     CDS             complement(685..1692)
                     /locus_tag="VEA63_08160"
                     /inference="COORDINATES: protein motif:HMM:NF025755.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-transfer associated ATP-grasp
                     domain-containing protein"
                     /protein_id="HYD84013.1"
                     /translation="MNFVQQDRRFGSGTNLALLKRGFFSERALLYPFDRYDPRWFVND
                     WAIEHRFQTINPSDVSIQLRNKFFLHLLLRELGYGDRTAPLVGVLTDGRFSSFSPYRK
                     LGDAIAAHGGVMAKPIAGASGLGVVRLGADDPVPPRGMFVVEGVLRQHAYAAAVFSGS
                     VNTIRVVTMRLGDKEPFIPAAAHRFGGKKSAPVDNFGRGGVSALVDLETGELSSGRSK
                     LHVHASTFHPVHPDTGVAVKGTTVPMWDEVKAFALELMKAIPGLQIAGWDICVTPDGP
                     RLIEGNGGVPSADLIQVHRPMLLDRRTREFFRAKRVISQQHFDELERMAAAGLERSDL
                     G"
     gene            complement(1873..>2182)
                     /locus_tag="VEA63_08165"
     CDS             complement(1873..>2182)
                     /locus_tag="VEA63_08165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD84014.1"
                     /translation="GAAGGVRAQAGFCENVTWVALKCVEVERESCAPETSNANPMQTV
                     EPSLALAQIDELTQLQLSVAQRADELMRLDRERRAERDYWREAEAEVLEARLGERRIE
                     "
BASE COUNT          433 a          764 c          623 g          362 t
ORIGIN      
        1 ggccgcgagc tgatgcgact cggctacgtc gcggtcgccg aaggcgagtt cgccacgctc
       61 gaactcacga ccgagggcat gacggtgctc gtgaaacgca ctccgatcac gctcacgaag
      121 ccgatggact tgccgaaaac aaaacgcgtc gcccgccgcg aaggcgacat cgcgtgcgac
      181 gaaatcctct tcgaacgcct gcgcggcttg cgcaagcagc tcgccgacga acgtcgcgtc
      241 cccgcgtatg tgatttttgg agacacgacg ctccgtgcca tggcgcgcca ttaccccgcg
      301 accgtcgatt cgctggaggg aattcccggc attggtgaga aaaagcgcgc cgaattcggc
      361 gagctcttcg cgactgaaat tgccgactat ctccgcacga actcccgcca gtccttcaac
      421 gactgaagcg ggattggtga ctcgctgcgt gagcagcggg acggccagcg ccgtctgcaa
      481 caagcgccgc cttcccgttg taggagcctg cttgcaggcg attgggtgcg cgaaacgcgg
      541 gtgcagcgta aatcgcctgc aagcaggccc caaccctctc aaatccgttg agccagcgcc
      601 cgatcatacg caccggcact cggttgagca cgtgccggtg aaacggctac tgctccacca
      661 atgccacggt ccaccggcat gatctcaccc caaatcagat ctctcaagcc ccgccgcagc
      721 catgcgctcc agttcgtcga aatgctgctg gctgatcacg cgcttcgcgc gaaaaaattc
      781 ccgcgtccgc cgatccagca gcatcggtcg atgcacctga atcaggtccg cgctcgggac
      841 gccgccgttg ccttcgatca aacgcggacc atccggcgtc acgcagatat cccagccggc
      901 gatctgcaaa ccgggaatcg ccttcatgag ctcgagcgca aacgccttca cctcgtccca
      961 catcggcacc gtcgttcctt tcacggcgac acccgtgtcg ggatgcaccg gatgaaaggt
     1021 cgacgcatgc acgtgaagtt tcgaacgtcc gctcgacagc tcgcccgtct cgagatcgac
     1081 caaggccgac actccgccgc ggccgaaatt atccaccggc gcggattttt tcccgccgaa
     1141 gcgatgcgcc gccgcgggaa tgaacggctc cttgtcgccc agccgcatcg tcacgacgcg
     1201 tatcgtgttc accgaccccg aaaacaccgc cgcggcatac gcatgctgcc gaagcacgcc
     1261 ttcgaccacg aacatgccgc gcggcggcac cggatcatcc gccccgagtc gcaccacgcc
     1321 cagaccgctc gcgcccgcga tcggcttcgc catcacgccg ccgtgcgcgg cgatggcgtc
     1381 gccaagcttc cgatacggtg agaacgagct gaatcgcccg tccgtcaaca cgccgacgag
     1441 cggcgccgtg cggtcgccgt agcccagttc gcgcagcagc aggtgcagaa aaaacttgtt
     1501 ccgcaactga atcgagacgt ccgacggatt gatcgtctga aaccggtgct cgatcgccca
     1561 atcattcacg aaccaacggg gatcgtaacg atcgaacgga tacaagagcg cgcgctccga
     1621 aaagaaaccc cgcttcagca gcgcgaggtt ggtgccggag ccgaagcggc ggtcctgctg
     1681 cacaaagttc atgacgcgct ccgcgcgatg aacgctttga cgcaacagtc ttcgggggtg
     1741 ggtcagccgc acggctccaa cgagcggcgc cgccgaacct ggtcaaggcg attgccggtc
     1801 cacggcccac gcccagcttt tccccgatga cattccttcc ggccaccggc ggaaaccgcg
     1861 gccgtgcgag tcttattcga tacgccgctc accgagtcgc gcctcgagga cctccgcctc
     1921 cgcttcgcgc caataatcac gctctgcccg gcgctcgcga tcgagccgca tcaattcgtc
     1981 ggcgcgttgc gccacgctga gctggagttg ggtcaactca tcgatctgag cgagagcaag
     2041 tgaaggctcg acggtttgca tggggttggc gtttgaagtt tcgggagcac aggactctcg
     2101 ctcaacttcg acacatttca aagccaccca cgtcacgttt tcgcaaaaac ccgcctgcgc
     2161 gcgcacgccg cccgctgcac ct
//
LOCUS       DASPLT010000786         2198 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_49317, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000786 DASPLT010000000
VERSION     DASPLT010000786.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 2198)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2198)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2198
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_49317"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            145..1230
                     /locus_tag="VEA63_08170"
     CDS             145..1230
                     /locus_tag="VEA63_08170"
                     /inference="COORDINATES: protein motif:HMM:NF021589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HYD84015.1"
                     /translation="MAPQTRALFTLNEGKELIFVSATYSIGLDTNVFTRSADQESFTQ
                     NFSFSADYSRQAGLISVTANIGTSTGRFESIRNQDFADPSLSVAFRKRYGRTTGSLRL
                     QTRRESQPDPDAGQRTEAWNHNMALDVRYPVNDRYFFTNGFSALTKLYTNKSSFSDLT
                     SFSDSIAINYAYTSKLDLNAAYKFGYSQTSHNTNAFDQSLTVGASGGILPKLSGSLNM
                     GVTRRANETSIGIDEVFTGFSSGTSLKWLYSRRLTFTADLNSDFSTTSTDITVLRTSG
                     GLHATASLSSKYIVGSGITYTISNFLGIAGAGRRDEMLQFDASLGLALTTHIRTSVAY
                     SYMLNRSNFPGSDFERHTAAFTITATY"
     gene            1238..1882
                     /locus_tag="VEA63_08175"
     CDS             1238..1882
                     /locus_tag="VEA63_08175"
                     /inference="COORDINATES: protein motif:HMM:NF014606.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HYD84016.1"
                     /translation="MLRSLRFLSRPFLLTLLALTATLSLRAQEQPGAAVETEKSNYVY
                     RLTITDRIRISIFQEDDLATMARVDARGNVNLKLVGDLYVAGMTVAEAQHAIEKAYRD
                     GRYLRNPQASIAIEDYAPREVSISGQVKAPGRYLLPIESTFSIVELVTKAGGLTDIAK
                     GTNVIVTRTSSDGKMTTLTVDVDALIRGRKSNNPDNAAILLEPGDVVYVPERII"
     gene            1886..>2198
                     /locus_tag="VEA63_08180"
     CDS             1886..>2198
                     /locus_tag="VEA63_08180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD84017.1"
                     /translation="MPIADPSPKPPVHVSGAREDDNTVERRTLRDYYIILRERVWIAL
                     PLALLVAVPMGYFKARETPMYASTATMQFEKPETVVTSQVVVDAAVRSDIELNPNIQI
                     LN"
BASE COUNT          441 a          814 c          516 g          427 t
ORIGIN      
        1 acgccgactc cggtcgtccc acccgtcctc gacagcaact tggtcaccag ccctgcgaat
       61 agttaactcg ctgcgccgta tccgtccatt gtctcgatga ctctcagttg gcgaatcagc
      121 agcagtttca tcctttgtat gatcatggct ccgcagacgc gcgctttgtt cacccttaat
      181 gaggggaagg aactgatttt cgtcagcgcc acgtatagca tcgggctcga cacgaacgtc
      241 ttcacccgct ccgccgatca ggagtcgttc acccaaaatt tttcgttctc cgccgactac
      301 agccgccaag ccggtttgat cagcgtcacc gcgaacatcg gcacctcgac cggtcgcttc
      361 gaatccatcc gcaaccagga cttcgccgac ccgagtctct cggtcgcgtt tcgcaaacgc
      421 tacggccgca ccaccggttc gcttcggctc cagacgcggc gcgaaagcca gcccgatccc
      481 gacgccggcc agcgcacgga ggcgtggaat cacaacatgg ctctcgacgt tcgttacccg
      541 gtcaacgacc gctatttttt cacgaacggg ttcagcgcgc tcaccaagct ctacaccaac
      601 aaatccagct tcagcgatct cacgtcgttc tccgattcca tcgcgatcaa ctacgcctac
      661 acgtcgaaac tcgacctgaa cgccgcctac aaattcggct acagccagac ttcccacaac
      721 accaacgcct tcgaccaaag cctcacggtc ggcgcctccg gtggcattct cccgaagctc
      781 agcggttccc ttaacatggg cgtaacccgc cgcgccaacg aaacctccat cggcatcgac
      841 gaggtcttca ccggcttctc ctccggcacc tcgttgaagt ggctttactc gcggcgcctc
      901 acgttcaccg ccgacctgaa ctccgatttc tccaccacct cgaccgacat caccgtcctg
      961 cgcacgagcg gcggcctgca cgccaccgcc tcgctgagca gcaaatacat tgtcggctcc
     1021 ggaatcactt acacgatcag caatttcctc ggcatcgccg gtgccggccg ccgcgacgag
     1081 atgctccagt tcgacgccag tctcggactc gcgctcacca cccatattcg caccagcgtc
     1141 gcctactcct acatgcttaa ccggtcgaac ttccccggct ccgatttcga gcgccacacc
     1201 gccgctttca cgatcaccgc cacgtattga tcccgccatg ctgcgctcgc ttcgctttct
     1261 ctcccgtcct tttctcctga cgcttctggc gctcacggcg acgttgtctc ttcgcgccca
     1321 ggaacagccc ggcgcagccg tcgaaacgga gaagtccaac tacgtctacc ggctgaccat
     1381 caccgaccgc attcgcattt cgattttcca ggaagacgac ctcgccacga tggcgcgcgt
     1441 cgatgcccgc gggaacgtta acctgaagct cgtcggcgat ctctacgtcg ccggcatgac
     1501 cgtcgccgaa gcccagcacg ccatcgaaaa agcgtatcga gacggccgat acctccggaa
     1561 tccccaggcc tccatcgcca tcgaagacta cgccccgcgc gaggtctcga tttccggtca
     1621 ggtcaaagct cccggccgct accttcttcc catcgaatcc acgttttcga tcgtcgaact
     1681 cgtcacgaaa gcaggcggtc tcaccgatat cgccaaaggc accaacgtca tcgtcacgcg
     1741 caccagctcc gatggcaaaa tgaccacgct caccgtcgac gtcgacgccc tcattcgcgg
     1801 tcgcaaatcg aacaatcccg acaacgccgc catcctgctc gagcccggcg atgtcgtcta
     1861 cgtgccggag cgcatcatct aattcatgcc gatcgccgat cccagtccca aaccgccggt
     1921 tcacgtcagc ggcgctcgag aagacgataa caccgtcgag cgtcgcacgc tgcgcgatta
     1981 ctacatcatt ctccgtgaac gcgtctggat cgctctcccg ctggctcttc tcgtcgccgt
     2041 tcccatgggc tatttcaagg cgcgcgagac gccgatgtat gcgagcacgg cgaccatgca
     2101 gttcgaaaaa ccggaaaccg tcgtcacttc gcaagtcgtc gtcgacgccg ccgtccgcag
     2161 cgacatcgag ctcaacccca acatccagat tctcaaca
//
LOCUS       DASPLT010000787         1808 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_213935, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000787 DASPLT010000000
VERSION     DASPLT010000787.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1808)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1808)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1808
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_213935"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..422)
                     /gene="rplE"
                     /locus_tag="VEA63_08185"
     CDS             complement(<1..422)
                     /gene="rplE"
                     /locus_tag="VEA63_08185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HYD84018.1"
                     /translation="MSYVPSLKKLYVEHVVPELIKSRGYKNKHQVPKLVKINLNTGID
                     SDADKNQIADIARDMGLIAGQKPILNKARKAIANFKLKPNQVVGCSVTLRGPSMWEFL
                     QRLLAVALPTIRDFRGVGSKLDGQGNYNLGITDHTIFP"
     gene            complement(532..807)
                     /gene="rplX"
                     /locus_tag="VEA63_08190"
     CDS             complement(532..807)
                     /gene="rplX"
                     /locus_tag="VEA63_08190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HYD84019.1"
                     /translation="MAQKFHVKRGDQVVVIAGSQKGKSGKVLEILAAKQRARVEGVGM
                     IKRHLKKSQEHPQGTIAEREGSIHISNLMPQATFDASKRKKTETASA"
     gene            complement(809..1174)
                     /gene="rplN"
                     /locus_tag="VEA63_08195"
     CDS             complement(809..1174)
                     /gene="rplN"
                     /locus_tag="VEA63_08195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HYD84020.1"
                     /translation="MIQLRSVIDVADNTGARKAAMISRKGQITDTAGVGDIITVHIKQ
                     SSTEATVKKGEVHKAVVVRTKAPVRRADGSYLRFDSNACVIIDATNNPRGTRIFGPVA
                     RELRAKNFMKIISLAPEVL"
     gene            complement(1209..1580)
                     /gene="rpsQ"
                     /locus_tag="VEA63_08200"
     CDS             complement(1209..1580)
                     /gene="rpsQ"
                     /locus_tag="VEA63_08200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HYD84021.1"
                     /translation="MSSSATGQRNTRRTQIGFVSSRSGDKSVKVTVAYKTPHPLYHKV
                     VNRQTVLHVHDEKNETKVGDKVEVMETRPLSRLKRWRIVSVIQKAITSDAVAISENDV
                     AAQVPTKTTSANAAAESAPQA"
     gene            complement(1610..>1808)
                     /gene="rpmC"
                     /locus_tag="VEA63_08205"
     CDS             complement(1610..>1808)
                     /gene="rpmC"
                     /locus_tag="VEA63_08205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007359191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HYD84022.1"
                     /translation="KEIRELAPAEITTKLRETREQLLQLRLRKHTGQVEKTHELRTLR
                     KDIARLETIQNEKKKKAPVAA"
BASE COUNT          272 a          474 c          638 g          424 t
ORIGIN      
        1 tccgggaaga tcgtgtggtc ggtgatgccg aggttgtagt tgccctggcc gtcgagcttc
       61 gacccgacgc cgcggaagtc gcggatggtc ggcagcgcga ctgcgagcag gcgctggagg
      121 aattcccaca tcgagggacc gcggagcgtg acgctgcagc cgacgacctg gttgggcttc
      181 agcttgaagt tcgcgatcgc cttgcgggcc ttgttgagaa tcggcttctg accggcgatc
      241 agacccatgt cacgggcgat gtccgcgatc tggttcttgt cggcgtcgga gtcgatgccg
      301 gtgttcaaat tgatcttaac cagcttcggc acctggtgct tgttcttgta accacggctc
      361 ttgatgagct cgggcacaac gtgctcgacg tagagttttt taagggaggg aacgtagctc
      421 attgggcgat gcccgcgatt accgaccgcg aggcgattga gaaggatgtt ttaagtggaa
      481 aagcgtcgag taacgcaggc gcgccgccgg gttggcaagc gtccagtgaa ctcaggcgga
      541 cgcggtttcg gttttcttgc gcttgctcgc gtcgaacgtc gcttgcggca tcaggttgct
      601 gatgtggatc gaaccttcgc gctcggcgat ggtgccttgc ggatgttcct gcgacttctt
      661 caggtgacgc ttgatcatgc cgacgccttc gacgcgggcg cgctgcttgg cggcgaggat
      721 ctcaaggact ttgccagact tgcccttctg ggagccggcg atcacgacga cttggtcgcc
      781 gcgtttgacg tgaaatttct gggccatgtt agagaacctc cggagcgagc gagatgatct
      841 tcatgaagtt cttcgcgcgc agttcgcggg cgacgggccc gaagatacgg gtgccgcgcg
      901 ggttgttggt cgcgtcgatg atgacgcacg cattgctgtc gaaacgcaga tagctgccat
      961 cggcgcgacg cacgggagcc ttggtgcgaa cgacgacggc cttgtggacc tcgcccttct
     1021 tcacggtggc ttcggtggag ctttgcttga tgtgaacggt gatgatgtcg cccacgccgg
     1081 ccgtgtccgt gatctggccc ttgcgggaga tcatggcggc tttgcgggca ccggtgttgt
     1141 cggcgacgtc gataacggag cgcagttgga tcatggcagg cctcctgtga gtttttagtt
     1201 gttagaaatt aggcctgcgg agcggactcg gcggcggcgt tggcggaagt ggtcttcgta
     1261 gggacctgcg cggcgacgtc attctcgctg atggcgacgg cgtcggacgt gatcgccttc
     1321 tggatcaccg acacgatgcg ccagcgcttc aggcggctga gcgggcgggt ttccatgacc
     1381 tcgaccttgt caccgacctt cgtctcgttc ttctcgtcgt ggacgtggag aacggtctgg
     1441 cggttgacga ctttgtggta aagcggatgc ggcgtcttgt aggccacggt gaccttgacg
     1501 gacttgtcgc cggagcggct ggagacgaag ccgatttggg ttctgcgcgt gttgcgctgg
     1561 ccggtagcgg aggaggacat ggcggtgaat tcggtttaac gcgcgtcgct caggcggcga
     1621 cgggcgcttt ctttttcttt tcgttctgga tggtttcgag gcgcgcgatg tccttgcgga
     1681 gcgtgcggag ctcgtgggtc ttctcgacct ggccggtgtg cttgcgcaga cggagctgca
     1741 gcagttgctc gcgcgtttcg cggagcttgg tggtgatctc ggccggagcg agttcgcgga
     1801 tttctttg
//
LOCUS       DASPLT010000788         1110 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_10197, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000788 DASPLT010000000
VERSION     DASPLT010000788.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1110)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1110)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1110
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10197"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..425
                     /locus_tag="VEA63_08210"
     CDS             <1..425
                     /locus_tag="VEA63_08210"
                     /inference="COORDINATES: protein motif:HMM:NF025663.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-grasp fold amidoligase family protein"
                     /protein_id="HYD84023.1"
                     /translation="RLPRHVMFEEFLGETEVVPRDYKFYVLHQKVRFISVDEGRFRNH
                     TRDLFQPDWTPITSRIGPHPRAMETPAAPTQLDRMVAVAEALAVDSDFLRVDLYVVRN
                     EIYFGELTHTPGAGNLFFEDPALNIELGNYWRQPVRYR"
     gene            complement(439..>1110)
                     /locus_tag="VEA63_08215"
     CDS             complement(439..>1110)
                     /locus_tag="VEA63_08215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRTAC1 family protein"
                     /protein_id="HYD84024.1"
                     /translation="RYAATELASGVFMSRGDGTFRFERLPRLAQIAPIFGMVAGDFDG
                     DGLLDLYAVQNSHAPMPIVGRFDGGLSVWLRGDGRGGFAVAPFAESGLVVPGDAKALA
                     ACDLDDDGWGDFVVTRNSGATLVFQNRGVAGRRALRVALRGGRGNPHAIGARITVELF
                     DGRTQALEVQAGAGYYSQSSAAGFFGYEEKRGPRAVRINWPSGKETQHEVTAGAARLT
                     LTERE"
BASE COUNT          214 a          405 c          292 g          199 t
ORIGIN      
        1 atcgcctgcc gcgtcacgtc atgttcgagg agtttctcgg cgagaccgag gtcgttccgc
       61 gcgattacaa attctacgtg cttcatcaaa aggtgcgctt catcagcgtc gacgaaggcc
      121 gtttccggaa ccacacacgc gacctcttcc aacctgactg gacgccgatc acctcacgca
      181 tcgggccgca cccacgcgca atggagacgc ctgcggcgcc cactcagctc gaccggatgg
      241 tcgccgtcgc cgaagctctg gccgtcgaca gcgactttct ccgcgttgat ctctacgtgg
      301 tccgcaatga aatctatttc ggcgaactca cccacacccc tggcgccggc aatctcttct
      361 tcgaagatcc cgcgctgaac atcgaactcg gcaactattg gcgtcagccc gtccgttatc
      421 gctagacgga agtttgccct actcgcgttc ggtcaatgtc agcctcgccg ctcccgcggt
      481 cacctcgtgc tgcgtctcct tgcccgacgg ccagttgatg cgaactgcgc gtggtccacg
      541 tttttcctcg tagccgaaaa agccggccgc gctcgattgg ctgtaatacc ccgcgccagc
      601 ctgcacttcg agcgcctgcg tgcgcccgtc gaaaagttcg accgtgattc tcgccccgat
      661 cgcgtgcgga ttgccgcgcc cgccacgcaa cgcaacgcgc aaagctcgcc gccccgcgac
      721 cccacggttt tgaaacacca gggtggcgcc actgttccgc gtgaccacga agtcacccca
      781 accgtcgtca tcgagatcac acgcagccag cgccttcgca tcacccggca cgacgaggcc
      841 gctttcggca aaaggcgcca cggcgaatcc tccacgtccg tcgccgcgca accagacact
      901 tagcccgcca tcgaagcggc ccacgatggg catcggcgcg tgcgaatttt gcacggcgta
      961 aaggtcgagc aaaccatcgc catcgaaatc acccgccacc atgccgaaga tcggtgcgat
     1021 ctgtgcgaga cgcggcagac gttcgaaacg aaacgtccca tcgccccggc tcatgaaaac
     1081 gccactcgcc agttccgtcg ccgcgtaacg
//
LOCUS       DASPLT010000789         2758 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_275137, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000789 DASPLT010000000
VERSION     DASPLT010000789.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 2758)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2758)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2758
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_275137"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            259..1101
                     /gene="lpxI"
                     /locus_tag="VEA63_08220"
     CDS             259..1101
                     /gene="lpxI"
                     /locus_tag="VEA63_08220"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376676.1"
                     /note="LpxI, functionally equivalent to LpxH, replaces it
                     in LPS biosynthesis in a minority of bacteria; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase LpxI"
                     /protein_id="HYD84025.1"
                     /translation="MLSAFLPPDFDPRRPLALIAGQGLYPQLVAEAVRRAGVPLKLIA
                     FDEETRPELVASFHETDRRTLLVGQLGKLLKTLREFDAGYAIMAGQISPRRLFKGLHP
                     DLKAVRILASLKRRNAETIFGAIAAEIENLGVRLLDARSFLDDQLATPGCMTGRSFPI
                     DSDYVDHGIHIARECARLDIGQGCVVRKGTVLAVEAYEGTDEMLRRAGTFKTDETLFV
                     KTVKAAQDYRFDVPCFGQRTLETMREAGLAAAALEANRVILLDRPAVLAQAKTWGIHL
                     LGFE"
     gene            1312..1782
                     /locus_tag="VEA63_08225"
     CDS             1312..1782
                     /locus_tag="VEA63_08225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nuclease family protein"
                     /protein_id="HYD84026.1"
                     /translation="MQNDVVPVSVKGVMPTANGCAVFLGNDEKTFVIYVDHSVGNAIQ
                     MTLNGVKKDRPLTHDLIGSILLGLGAQLDHIIVNDAREGTFFARILLRMENELGKKIV
                     EIDARPSDSIVLALQQKRPLFVARAVLDAAEDMTEILDRVRQQQAEESGEEEQP"
     gene            1875..>2758
                     /locus_tag="VEA63_08230"
     CDS             1875..>2758
                     /locus_tag="VEA63_08230"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase family protein"
                     /protein_id="HYD84027.1"
                     /translation="MQDVTDLAFMRVIAHYGAPDYFFTEFFRVHAQSRPEKHILRSID
                     ENTTGRPVFAQLIGEDLHHLARTAAELQQHPIAGIDLNLGCPAPKVYKKNVGGGLLRD
                     PARVDEILATLRAATPGLFTVKMRIGFDDTTPFEPMLDLVNTHRVDLLSIHGRTVKEM
                     YRSDVHYDYIARAVSRVGCPVLANGNILSPDRAANVLADSCAAGVMIGRHAIRNPWIF
                     RQCRERFAGSAIFQPTLVDVRDYIERLYRATQTPGLPERLHVNKMKKYLNFVGQSVDP
                     DAAFLYEMRRSESEADLFA"
BASE COUNT          521 a         1061 c          668 g          508 t
ORIGIN      
        1 cgcctttaca tgaaggcgct tcggaacggc aaaaacgaag tcctcggcgt cgtcctcaac
       61 ggcctctctc cgcgccgcat cgagtattac tcgaactacc gctactaccg gagctacaaa
      121 aaatactacg gctcccagtc ctaaaaactg aaagctgaga tgctgaaaag ctgaaacaga
      181 gccggcgttc cgccctcctg cgaacgcagc tcgccaattt cagcttttca gtctttcagc
      241 ttttcagctt tttccgccgt gctctccgcg tttctcccgc ccgacttcga tccccgccgc
      301 ccgctcgccc tcatcgctgg ccagggcctc tatccgcaac tcgtcgccga ggccgttcgc
      361 cgcgccggcg ttccgctgaa actcatcgcc ttcgacgagg aaacccgccc ggagctcgtc
      421 gcctcctttc acgaaaccga tcgccgcacg ctcctcgtcg gccagctcgg caagctcctc
      481 aagacgctcc gtgagttcga cgccggttac gcaatcatgg ccggccagat ttccccgcgc
      541 cgtctcttca aaggtctcca tcccgacctc aaagccgttc gcatcctcgc gtcgctcaaa
      601 cgtcgcaacg ccgaaaccat cttcggcgcc atcgccgccg aaatcgaaaa cctcggcgtg
      661 cgcctgctcg acgcccgctc ctttctcgac gaccagctcg ccacacccgg ctgcatgacc
      721 ggccgctcgt ttcccatcga ttccgactac gtcgatcacg gcatccacat cgcccgcgaa
      781 tgcgcgcgcc tcgacatcgg ccagggctgc gtcgtgcgca aaggcaccgt tctcgccgtt
      841 gaagcttacg aaggcaccga cgaaatgctc cgccgcgccg gcacgttcaa gaccgacgaa
      901 acgctcttcg tcaaaaccgt caaagccgcg caagactacc gcttcgacgt cccatgtttc
      961 ggtcaacgca cactcgagac gatgcgcgaa gccggcctcg ccgccgccgc actcgaagcg
     1021 aaccgcgtca tcctcctcga tcgccccgcc gtcctcgccc aggcgaaaac ctggggcatc
     1081 cacctcctcg gcttcgagta gccccaggcc tctgcgcccg cccgcgcttc gctttcgcgg
     1141 cgctcccgcc caccaccctt tgtcatctaa tagatgacaa actcccccgc gcggtcctcg
     1201 ccgccgcatg cgctctttcg ttctccccgc atcaacaccc gccggcaccg gaacatccgc
     1261 acctgcctcc cgccgacttg cgctttcccc gcgtccggcc atttcttccg catgcagaat
     1321 gatgtggtcc ccgtttccgt aaaaggcgtg atgcccaccg ccaacggctg tgccgtcttc
     1381 ctcggtaacg acgagaaaac gttcgtcatc tacgtcgacc attccgtcgg caacgccatc
     1441 cagatgaccc tcaacggcgt gaaaaaagac cgcccgctta cgcacgattt gatcggcagc
     1501 atcctcctcg gcctcggcgc ccagctcgac cacatcatcg tcaacgacgc ccgcgaaggc
     1561 acgttcttcg cacgcatcct ccttcgcatg gaaaacgagc tcggcaaaaa aatcgtcgag
     1621 atcgacgcac gcccgagcga ttccatcgtc ctcgctctcc agcaaaaacg cccgctcttt
     1681 gtcgcccgcg ccgtgctcga cgccgccgag gacatgaccg aaatcctcga ccgcgtccgc
     1741 caacaacaag ccgaagaatc cggcgaggaa gaacagccgt gagcggcgaa tcatcgcccc
     1801 attcttcccc gaccgcacgc gctccgcttc cggccggtca acaccccggc ggtccactca
     1861 ccgcgctcgc gccgatgcag gacgtcaccg atctcgcgtt catgcgcgtg atcgcccact
     1921 acggcgcgcc ggattatttt ttcaccgagt ttttccgcgt tcacgcgcaa tcccgccccg
     1981 agaagcacat ccttcgctcg atcgacgaga acaccaccgg ccgcccggtt ttcgcgcagc
     2041 tcatcggcga ggaccttcat cacctcgccc gcaccgccgc cgaactccag cagcatccga
     2101 tcgccggcat cgatctcaac ctcggctgcc ccgctccgaa ggtttacaag aaaaacgtcg
     2161 gcggcggact cctccgcgat cctgcgcgcg tcgacgaaat cctcgccacg ctccgcgccg
     2221 ccacgcccgg attgttcacc gtgaaaatgc gtatcggctt cgacgatacg acgcccttcg
     2281 agcccatgct tgatctcgtc aacacacacc gggttgacct gctcagcatt catgggcgca
     2341 ccgtgaagga aatgtatcga agcgacgttc actacgacta catcgcgcga gcggtttcgc
     2401 gcgtcggctg ccccgtgctc gccaatggca acattctctc ccccgaccgt gctgccaatg
     2461 ttctcgccga tagttgcgcc gccggcgtga tgatcgggcg ccacgccatt cgcaacccct
     2521 ggatcttccg gcagtgtcgc gaacgtttcg cgggctcagc cattttccag cccaccttgg
     2581 tcgatgtccg cgattacatc gagcggctct atcgtgcgac gcaaacaccc ggcctgcccg
     2641 agcgcctcca cgtgaacaag atgaagaaat atctcaactt cgtcggccaa agcgtggatc
     2701 ccgacgccgc tttcctctac gaaatgcgcc gctccgaatc cgaagcggac ctcttcgc
//
LOCUS       DASPLT010000790         1813 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_255637, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000790 DASPLT010000000
VERSION     DASPLT010000790.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1813)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1813)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1813
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_255637"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..536
                     /locus_tag="VEA63_08235"
     CDS             <1..536
                     /locus_tag="VEA63_08235"
                     /EC_number="1.1.1.30"
                     /inference="COORDINATES: protein motif:HMM:NF009093.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003858 - 3-hydroxybutyrate dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="3-hydroxybutyrate dehydrogenase"
                     /protein_id="HYD84028.1"
                     /translation="IDILVNNAGIQFVSPVEEFPPERWQAIQDIVLNASFHAIRAAVP
                     AMKREGWGRIINLASAHGLVASPFKSAYVAAKHGQIGLTRSVALELAETPITCNAVCP
                     GYVRTPLVEAQIESQASAHGLPRQRVIRDVILAAQPTRRFVEVDEVAALVVFLCGEAA
                     RSITGAAIPIDGGWTAR"
     gene            872..>1813
                     /locus_tag="VEA63_08240"
     CDS             872..>1813
                     /locus_tag="VEA63_08240"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HYD84029.1"
                     /translation="MNQNQDRNRLCRLLCGAALAFAATTSHVMAQTTSSSNAGNSEDD
                     IVLLSPFSVTGEDDEGYRATTTLAGTRIKTELRDVGSAITVVTDQFLKDTAVTGNEEL
                     LLYTPSTEVGGLNGNFSGGTRVRGLGAADNARDFFLSDIPWDSYNVDRIDLQRGPNSI
                     LFGLGKPSGLVNASLKTAGYKNENSVEARVGSFGSFRGVLNLNRVLLPNELAIRFDAV
                     KDHTKYRQEPAYGESRRMFGALRYDPMFLRKGSAHTTLRASYEKGLGHGNAPLTTPPI
                     DAITPWFKTGTTTTPDGRVFNNLNQGTFDWRYNTVYLP"
BASE COUNT          357 a          564 c          549 g          343 t
ORIGIN      
        1 cgatcgacat tctggtcaac aacgcaggca ttcagttcgt gtctcccgtc gaggaatttc
       61 cgccggagcg atggcaggcg atccaggata tcgtgttgaa cgcgagcttt catgcgattc
      121 gcgcagcggt gccggcaatg aagcgcgaag ggtggggccg catcatcaat ctcgcctcgg
      181 cgcacgggct cgtggcgtcg ccgttcaaga gtgcctacgt ggccgcgaag cacggccaga
      241 tcgggttgac gcgcagcgtc gcgctcgaac tcgcggagac tccaatcacg tgcaatgcgg
      301 tgtgtccggg ctacgtgcgc acgccgctcg tggaggcgca gatcgaaagt caggcgagcg
      361 cgcacggcct cccgcgccag cgcgtaattc gcgatgtcat tctcgcggcg cagccgacgc
      421 gacggtttgt ggaagtggat gaagtggccg cgctggtcgt gtttctttgc ggcgaagcgg
      481 cgcgctcgat cacgggcgcg gcgatcccga ttgacggcgg ttggacggcg cggtgaacga
      541 acgccacgtc tcatcggtga tccgttttgt tactgaatcg taactacaag cgcagcgaag
      601 gacgatggct tgcaccaagc gtgagattat agtggcagcg tttgagtggt aaggcgtagc
      661 ggtcggaaga tgaaaactgg catgctgtcc ggcgacgcaa gaattgcgtg ccacgcgctc
      721 cctttgtttc cgcgatctct ctttcgagat gaaaaccccc gaccgccttt ttcgccggat
      781 aactcgatcc gccgtgcggc gctccgacat tgagccagcc tgcggccgtc ccgttgattt
      841 cttcagtcag cccaccatcg aaccgccact aatgaaccag aaccaagatc gaaatcgcct
      901 ctgccgcctg ctgtgcggcg cggctctcgc gttcgcggcc acgacgtctc acgtcatggc
      961 gcaaacgact tcctcctcca acgcaggcaa cagcgaagac gatatcgtcc tactttctcc
     1021 gttctccgtc actggcgaag acgacgaagg ttatcgcgca acgaccacgc tggccggcac
     1081 gcgcatcaaa accgaactgc gtgacgtagg ctccgcgatc acggtcgtca ccgatcagtt
     1141 tctgaaggac acggccgtga ccggcaacga ggagcttctg ctctatacgc cgagcacgga
     1201 agtcggcgga ttgaacggca acttcagcgg cggcacgcgc gtgcgcggtc ttggcgccgc
     1261 ggacaacgcc cgcgactttt ttctgtcgga catcccgtgg gattcctaca atgtcgatcg
     1321 catcgatctg cagcgcggcc ccaattccat tctcttcggc ttgggaaagc cctccggcct
     1381 ggtcaacgcg agtttgaaga ccgcgggcta caagaacgaa aattcggtgg aggcgcgcgt
     1441 cggcagtttc gggagcttcc gcggcgtgtt gaacctgaac cgcgtgctgc tgcccaacga
     1501 actcgcgatc cgattcgatg cagtgaagga ccacacgaag tatcggcagg aaccggcgta
     1561 tggcgagagc cgccgcatgt ttggcgctct gcgttacgat ccgatgtttc ttcgaaaagg
     1621 atctgcccac accacgctac gcgccagcta cgagaaggga ctcggccacg gcaatgcgcc
     1681 gctgaccacg ccgccgatcg acgccatcac gccttggttc aaaacgggga cgacgaccac
     1741 gccggatgga cgcgtgttca acaacctcaa tcagggcacc ttcgactggc gctataacac
     1801 cgtctacctt ccg
//
LOCUS       DASPLT010000791         1207 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_255648, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000791 DASPLT010000000
VERSION     DASPLT010000791.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1207)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1207)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1207
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_255648"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..529
                     /locus_tag="VEA63_08245"
     CDS             <1..529
                     /locus_tag="VEA63_08245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009508136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SufD family Fe-S cluster assembly protein"
                     /protein_id="HYD84030.1"
                     /translation="VHLGARQARHESLSQLQAPGGFSEMLAVTVAEDTQEFDQRTLQI
                     HQAPNTKSDLLYKNTLRDKSRTIFSGLIVVDQDAQKTDAYQSNRNLMLSDDAEANSLP
                     GLEIQANDVRCTHGATSSRIDPEQEFYLQSRGIGKAAADELLTFGFFEEVLSRLADES
                     LHDALRTLIQTKFKK"
     gene            608..1174
                     /locus_tag="VEA63_08250"
     CDS             608..1174
                     /locus_tag="VEA63_08250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007358967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein"
                     /protein_id="HYD84031.1"
                     /translation="MSTNRDRILSRDVVATQIPSGDRADLTAGTEVMIHQTLGGSYTV
                     QTDSGLFRIDGKDGDALGEQVVDNSVKAATLSDGAPDPEAIWGQLRKVFDPEIPVNIV
                     DLGLVYSMDVEAINGPSPDDKPSYKVNVAMTLTAPGCGMGPAIAEDAKGKILLVPGVA
                     DADVRITWEPAWNQAMISEEGKMKLGLI"
BASE COUNT          252 a          447 c          313 g          195 t
ORIGIN      
        1 cgtccacctc ggcgcccgcc aggcccgcca cgaatcgctc tcgcaactcc aggctcccgg
       61 cggtttttcc gagatgctcg cggtcaccgt cgccgaggac acccaggagt tcgaccagcg
      121 cacgctccag attcaccaag cgccgaacac gaagtccgat ctgctctaca aaaacacgct
      181 gcgtgacaaa tcccgcacca ttttctccgg cctgatcgtc gttgatcagg acgcgcaaaa
      241 aaccgacgcg tatcagtcga accgcaacct gatgctcagc gacgacgccg aagcgaactc
      301 gcttcccggt ctcgagattc aggccaacga cgtccgttgc acgcacggcg cgacctcctc
      361 gcgcatcgat cccgagcagg agttttatct ccaatcgcgc ggcatcggca aagcggccgc
      421 cgacgaactc ctcacctttg gtttcttcga ggaagtcctc tcccgcctcg ccgacgagtc
      481 gctccacgac gccctccgca ccctcatcca aacgaaattc aagaaatgaa cctcccgcga
      541 atcacgcgaa tcgacccgaa tgcttttggc tattcgcgat cgttcgcgta attcgcgggc
      601 cactccgatg agcaccaatc gcgatcgcat tctctcccgc gacgtcgtcg ccacccaaat
      661 tccctccggc gaccgcgccg acctcaccgc cggcaccgag gtgatgattc accagacgct
      721 cggtggcagc tacacggtcc agaccgacag cggcctcttc cgcatcgacg gcaaggacgg
      781 cgacgcgctc ggcgagcagg tcgtcgacaa ctccgtcaaa gcggccacgc tctccgacgg
      841 cgcgcccgat ccagaagcca tctggggcca gctccgcaaa gtcttcgatc ccgaaatccc
      901 ggtaaacatc gtcgacctcg gcctcgttta ctcgatggac gtggaagcga tcaacggccc
      961 ctcgcccgac gacaaaccgt cctacaaggt caacgtcgcc atgacgctca ctgcccccgg
     1021 ctgcggcatg ggtccggcca tcgccgaaga cgccaaagga aaaatccttc tggtccccgg
     1081 cgtcgccgac gccgacgtcc gcattacgtg ggaacccgcc tggaaccagg cgatgatctc
     1141 cgaagagggc aaaatgaagc tcggcctcat ttgaggccag cccgccgaag gtggagcccg
     1201 acgtccc
//
LOCUS       DASPLT010000792         1647 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_174102, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000792 DASPLT010000000
VERSION     DASPLT010000792.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1647)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1647)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1647
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_174102"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1497
                     /locus_tag="VEA63_08255"
     CDS             <1..1497
                     /locus_tag="VEA63_08255"
                     /inference="COORDINATES: protein motif:HMM:NF013225.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HYD84032.1"
                     /translation="RGGSDEAREKHLSRGKLLVRDRVDALLDPGAPWLELSPLAAHGL
                     YNDDVPAAGIVTGIGRVSGRECVIVANDATVKGGTYYPLTVKKHLRAQEVARENRLPC
                     IYLVDSGGAYLPAQDDVFPDREHFGRIFYNQATLSGAGIAQIAVVMGSCTAGGAYVPA
                     MSDEAIIVKNQGTIFLAGPPLVKAATGEQVAAEDLGGGDLHTRVSGVADHLADNDAHA
                     LLLARRIVANLNRVKTPSIELAEPEEPRYAAEEIYGVIPADLRKSYDVREVIARIVDG
                     SRFDEFKVRYGTTLVTGFARIYGHPVGIVANNGILFSESAVKGAHFVQLCAQRGIPLI
                     FLQNISGFMVGRQYEAEGIAKHGAKMVAAVACAKVPKFTVVIGGSFGAGNYGMCGRAF
                     SPRFLWMWPNARISVMGGEQAAGVLATLKRDAMTATGRTWTEEDDEAFKAPIRRQYET
                     QGHPYYATARLWDDGVIDPAQTRRVLALGLSAAMNAPMEETRFGVFRM"
BASE COUNT          288 a          464 c          599 g          296 t
ORIGIN      
        1 cgcggcggca gcgatgaggc tcgggagaaa catctttcgc gggggaaact cctggtgcgc
       61 gaccgcgtgg acgcgctgct ggatccgggc gcgccctggc tcgaactttc gccgctcgcc
      121 gcgcacgggc tgtataacga cgacgtgccc gcggcgggga tcgtcaccgg cattggccgg
      181 gtgagcgggc gtgaatgcgt gatcgtggcg aacgacgcga cggtgaaggg cggcacgtat
      241 tatccgctca cggtgaagaa acaccttcgc gcgcaggaag tcgcgcggga aaatcggttg
      301 ccctgtatct atctcgtgga ttcaggcggc gcgtatctgc ccgctcagga cgatgttttc
      361 cccgaccggg agcatttcgg ccgcatcttt tacaatcagg cgacgctctc cggagccggc
      421 attgcgcaaa tcgcggtggt gatgggttcg tgcaccgcgg gcggtgcgta tgtgccggcg
      481 atgtcggacg aggcgatcat cgtgaagaac caggggacga tttttctcgc cggcccgccg
      541 ctggtgaagg cggccacggg tgagcaggtc gcggcggagg atctcggcgg cggcgatctc
      601 catacccggg tgtcgggagt cgcagatcat ctcgccgaca acgacgcgca tgcgctgttg
      661 ctcgcgcggc gcattgtcgc gaacctgaac cgggtgaaaa cgccgagcat cgagctcgcg
      721 gagcccgagg agccgcgcta cgcggcggag gagatctacg gcgtgattcc ggcggacctg
      781 cgaaagtcct acgacgtgcg ggaggtgatt gcgcggatcg tcgacggttc gcgtttcgac
      841 gagttcaagg tccgttatgg cacgacgctg gtgacgggtt ttgcgcggat ttacgggcat
      901 ccggtcggca tcgtggcgaa caatggcatc ctgttttccg agtcggcagt gaagggggct
      961 cactttgtgc agttgtgcgc gcaacgcggc atcccgctga tttttctgca gaacatttcc
     1021 ggcttcatgg tggggcggca gtatgaggcc gagggcatcg cgaagcacgg cgcgaagatg
     1081 gtcgcggcgg tcgcgtgcgc gaaggtgccg aagttcaccg tcgtgatcgg cggaagcttc
     1141 ggtgcgggca actatggcat gtgtggacgg gcgttctcgc cgcgcttttt gtggatgtgg
     1201 cccaacgcgc gcatttccgt gatgggcggc gaacaggcgg cgggcgtgct cgcgacgctg
     1261 aaacgcgacg cgatgacggc aacgggacgg acgtggaccg aggaggacga tgaggccttc
     1321 aaggcgccga ttcgccggca atacgagacg cagggccatc cgtattacgc gacggcgcgg
     1381 ctgtgggacg acggcgtgat cgatccggcg cagacgcgac gcgtgctcgc gctcggactt
     1441 tcggcggcga tgaacgcccc gatggaggaa acccgtttcg gcgtgttccg catgtgagcg
     1501 aaagatcatg aaacctcgca ctctggagat tgaaatcgaa accggcgtcg gtcggatctg
     1561 gctgaaccgt ccggaggtgc acaatgcctt cgacgagata gcgatcgccg agctggtcga
     1621 cgcggtggcc caacttgaaa ccgatcc
//
LOCUS       DASPLT010000793         2555 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_153005, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000793 DASPLT010000000
VERSION     DASPLT010000793.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 2555)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2555)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2555
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_153005"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..387
                     /locus_tag="VEA63_08260"
     CDS             <1..387
                     /locus_tag="VEA63_08260"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002387688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HYD84033.1"
                     /translation="GGHVNHSFFWKVIGPRGGGAPRGELADAIAEKFGSFDALKTQFG
                     DAAAKRFGSGWAWLSVKGGALTVHSTANQDSPLIEGAMPVLGIDVWEHAYYLKYQNRR
                     GDYVSAFWNVVNWEQANANYRAARAG"
     gene            complement(540..1058)
                     /locus_tag="VEA63_08265"
     CDS             complement(540..1058)
                     /locus_tag="VEA63_08265"
                     /inference="COORDINATES: protein motif:HMM:NF013629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HYD84034.1"
                     /translation="MIASTQTNNPLADAKAAGDKTQSPLNLACSRLKAAGLRITQPRI
                     AILAALIKRGQPTTIEQIHADLEAGSCDLVTVYRCLSAFEDIGLVRRSFYHNGTSLYA
                     LSLGESHPYHVICKETNRVQEIDPATTAELRRNVQQIEELLKSRGYNNVTHVVEFFGL
                     APQETADTAAKN"
     gene            1210..1638
                     /locus_tag="VEA63_08270"
     CDS             1210..1638
                     /locus_tag="VEA63_08270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Minf_1886 family protein"
                     /protein_id="HYD84035.1"
                     /translation="MQDPDFADIVSLICKEDPRFDRKAYDFIRLGLDHTVKELKKREN
                     AKSSRSRHVSGPELLEGLRVYALDQFGPLAKTVLNSWGIRRCSDFGDIVFNLIEYNVF
                     SKTDNGRREDFSDLYTFEDAFVKPFRPEKSARGSSSVEAA"
     gene            1664..>2555
                     /locus_tag="VEA63_08275"
     CDS             1664..>2555
                     /locus_tag="VEA63_08275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HYD84036.1"
                     /translation="MAKSSSAAADFRLLDAFWREPIERIVLPNGLTLLLKPDRSAALA
                     SVQVWVKTGSTHEDAHLGAGLSHYLEHMLFKGTERRAGRDISATVQAHGGYINAYTTF
                     DRTVYYIDLPSEHTAVAIDLLADAVLHSTLPADECAKEKDVILREIAMTKDDPDNRLW
                     DALFATSFREHPYRQPIIGHRDVFSEVTREDLVGYYRARYVPNNLVVVIVGDIDVAAT
                     RAAVEQHFGAAPRAKLAPVLVPTEPLQLGARAEHRFEDVEITRAVLAWPIPGLAHEDT
                     PVLDLLSTVLGGGDSSVLWQD"
BASE COUNT          463 a          722 c          852 g          518 t
ORIGIN      
        1 ggcggtcacg tgaaccattc gtttttctgg aaggtgattg gtccccgcgg cggcggtgcg
       61 ccgcgtggcg agttggcgga tgcgatcgcg gaaaaatttg gctcgttcga tgcgctcaag
      121 acgcagttcg gcgacgcggc ggcgaagcgg tttggcagcg gctgggcgtg gttgagcgtg
      181 aagggtggag cgctcacggt gcattcgacg gcgaaccagg attcgccgtt gatcgagggt
      241 gcgatgccgg tgctgggcat cgacgtgtgg gaacacgctt actatttgaa gtatcagaat
      301 cgccgcggcg attacgtgtc ggcgttttgg aacgtcgtaa actgggaaca ggcgaacgcg
      361 aattatcgcg cggcgcgggc ggggtaggaa gggagagtga aagtgaaagg ggagcgggag
      421 tgagcgtgag gggacggttt gttcactctc actctcactt tcattctccc tcgctccgcg
      481 cgggcacaaa aaagggcggg tctgagaccc gcccaaggtg agtgaagctg cgatcagcgt
      541 cagttttttg cggcggtgtc ggccgtctct tgcggagcga ggccgaagaa ttcgacaacg
      601 tgcgtgacgt tgttgtaacc gcgggacttc aagagctcct cgatctgctg cacgttgcgg
      661 cgcagttcgg cggtggtcgc cggatcgatt tcctgcacgc gattcgtttc cttgcagatc
      721 acgtggtagg gatggctttc gccgaggctg agcgcgtaaa ggctcgtgcc gttgtggtag
      781 aaggaacggc gcacgaggcc gatgtcttca aaggcactca agcagcgata gacggtgacc
      841 agatcgcagg agccggcctc gaggtcggcg tggatctgtt cgatcgtcgt gggctggccg
      901 cgcttgatca acgcagcgag gatcgcgatg cgtggttgcg tgatacgcag gcccgccgcc
      961 ttgaggcgcg agcacgcgag attgagtggc gattgggtct tgtcgccggc ggctttcgca
     1021 tccgcgagcg ggttgttcgt ttgagtcgat gcaatcattt cacccatgag ggtgaggcgt
     1081 gagcgtcgag cttcgagtct ataatcatga gtttgcatca ggtctttccc tgaggaggtt
     1141 ccggttgaat gcgacgaccg ggttgctagt tgcacgagcg cagcggctgt gctatttccg
     1201 gccgacaaca tgcaagaccc ggactttgcc gatatcgtca gcctgatctg caaggaagat
     1261 ccgcgtttcg atcgaaaggc ttacgacttt atccgcctcg gcctcgacca cacggtcaag
     1321 gagctgaaga agcgcgagaa cgcgaagtcg tcgcgttcgc ggcacgtgtc ggggcctgaa
     1381 ttgctcgagg gcctgcgagt ctacgcgctc gatcaattcg gaccgctggc gaagaccgtg
     1441 ctcaactcgt ggggcatccg ccgttgctcg gatttcggcg acatcgtttt caatctgatc
     1501 gagtataatg tcttcagcaa aaccgacaac ggccggcggg aagacttctc ggatctctac
     1561 actttcgaag acgcgttcgt gaaaccgttc cggccggaaa aatccgcccg cggttcgtcc
     1621 agcgtcgaag cggcctgagc cggctcgggc gctcttatca ttcatggcaa aatcgtcctc
     1681 cgctgcggcg gatttccgct tgctcgacgc cttctggcgc gagccgatcg aacgcattgt
     1741 gctgccaaac ggcctcacgc ttctgctgaa gcccgaccgt tccgccgcac tcgcgtcggt
     1801 ccaggtttgg gtgaaaaccg gcagcacgca cgaggacgcg catttgggcg cggggttgtc
     1861 gcattatctc gagcacatgc tgttcaaagg cacggagcgt cgtgccggcc gcgacatctc
     1921 ggcgacggtg caagcgcacg gcggctacat caacgcttac acgacattcg accgcacggt
     1981 ttactacatc gacttgccga gcgaacacac cgccgtcgcg atcgacctgc tcgcggacgc
     2041 ggtgctgcac tcgacgctgc ccgccgacga atgcgcgaag gaaaaggacg tgatcctgcg
     2101 cgagatcgcg atgacgaagg acgatccgga caaccggctg tgggacgcgc tgttcgcgac
     2161 gtcctttcgc gagcatccct atcgccagcc gatcatcggg catcgcgatg tgttctccga
     2221 ggtcacacgc gaagatttgg tcggttacta tcgcgcccgc tacgtgccga acaatctcgt
     2281 cgtcgtgatc gtcggcgaca tcgatgtcgc ggcaacccgt gccgcggtcg aacaacactt
     2341 cggcgccgcg ccgcgggcga agctcgcgcc ggtgctcgtg ccgaccgagc cgttgcaact
     2401 tggcgcgcgg gccgaacacc ggttcgagga tgtggaaatc acgcgcgctg tgctcgcgtg
     2461 gccgataccg ggccttgcgc acgaagatac cccggtgctc gatctgcttt cgacggtgct
     2521 cggcggtggc gacagttccg tgttgtggca ggaca
//
LOCUS       DASPLT010000794         1307 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_133075, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000794 DASPLT010000000
VERSION     DASPLT010000794.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1307)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1307)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1307
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_133075"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1268)
                     /gene="infB"
                     /locus_tag="VEA63_08280"
     CDS             complement(<1..1268)
                     /gene="infB"
                     /locus_tag="VEA63_08280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009514570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HYD84037.1"
                     /translation="MPPTPRVAPPAPPPLPGSSPIPLPATPSAAPAETPSAPGEIKTI
                     HIKPPIVVRDFATALGLKPFRLISELNQAGGFASMNSNIDEAVAVKVAEKHGFILEIK
                     HRGDPAVQQAQKDKKEKKPEEDESKFLEPRPPVVCILGHVDHGKTSLLDAIRKANVAA
                     GEAGGITQHIGAYQIEFNGRKITFLDTPGHAAFSKMRARGAEVTDIAVLVVAADDGFM
                     PQTDEALKHAQNAKVALIVAVNKMDVKGANIEQVKGQMQQRNIAPEDWGGETITVPVA
                     AIKGQGIDNLLEMILLQADVLELKANPKAEASGVVIESQVDVGRGPLATVIVQRGTLR
                     VGDALVCGPNYARVRALFNDKGENLKEAPPATPVRVIGWSGTPDSGAIFRAVKNAREA
                     EKLAEEAQHQLRKATTTSDAAPKEVSVEQL"
BASE COUNT          210 a          429 c          401 g          267 t
ORIGIN      
        1 aagagctgtt ctacggaaac ttccttcggc gcggcgtcgg aggtcgtcgt cgctttgcgg
       61 agctgatgtt gtgcctcttc cgcgagcttc tcggcttcac gcgcgttttt cacggcgcgg
      121 aaaatcgctc cactgtcggg cgtgccggac cagccgatca ctcgaaccgg agtcgccggg
      181 ggcgcttcct tgaggttttc gcccttgtcg ttgaacaacg cacgaacgcg cgcgtagttc
      241 ggtccgcaca cgagtgcgtc gccgacgcgc aacgtcccgc gttgcacgat cacggtcgcc
      301 agcggaccgc ggcctacgtc gacctgcgat tcgatgacga cgccgctcgc ttccgctttc
      361 ggattggcct tcagctcgag cacgtccgcc tggagcagaa tcatctcgag gagattgtcg
      421 atgccctggc ccttgatggc cgcgaccggc acggtaatcg tttcaccgcc ccaatcttcc
      481 ggagcgatgt tgcgctgctg catctgcccc ttcacctgct cgatattcgc gcccttcacg
      541 tccatcttgt tgacggcaac gatcagcgcg accttcgcat tttgcgcatg cttcaacgcc
      601 tcgtccgtct gcggcatgaa gccgtcatcg gcggccacga cgagcaccgc gatatcggtg
      661 acttcggcgc cgcgcgcgcg catcttcgaa aacgccgcgt gaccgggcgt atccagaaaa
      721 gtgatcttcc ggccgttgaa ctcgatctgg taagcgccga tgtgctgcgt gatgccgccc
      781 gcttcgcccg cggcgacgtt cgccttgcgg atcgcatcga gcaacgacgt cttgccgtgg
      841 tcaacgtggc cgagaatgca gaccaccggc ggacgcggct cgaggaactt cgactcgtcc
      901 tcctccggct tcttttcctt tttgtccttc tgcgcctgct gcaccgccgg atcgccgcgg
      961 tgcttgatct cgagaatgaa gccgtgcttc tccgccacct tgacggcgac cgcttcgtcg
     1021 atgttcgaat tcatcgaagc gaaaccgccc gcctggttga gctctgaaat caaacggaac
     1081 ggtttcaggc cgagcgccgt cgcgaaatcg cgaacaacaa tcggtggctt gatatgaatg
     1141 gttttgatct cgcccggcgc ggacggcgtt tcggcgggcg ccgcagaggg cgtggccggc
     1201 aacggaatcg gcgaactgcc cggcaacggc ggcggagccg gcggtgcgac gcgaggcgtc
     1261 ggcggcaact gaaccggcgc ggaaacatgg ctcggcgctt tggcaac
//
LOCUS       DASPLT010000795         2194 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_33505, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000795 DASPLT010000000
VERSION     DASPLT010000795.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 2194)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2194)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2194
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_33505"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..476)
                     /locus_tag="VEA63_08285"
     CDS             complement(<1..476)
                     /locus_tag="VEA63_08285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD84038.1"
                     /translation="MRRTSLLLLLGASLLIAAPALANARKPAVSLRETVAVTPFRDLA
                     EAREFRIEGSADERTGKRVWLRVTDGIGKTVTAQVAIADGKFSAKYPNDFANAASLWP
                     AVYFVDAAPAENAPAEERAEAAVLVYDSRTGRLPELPSAFTTDLRAADGSVDAAAP"
     gene            complement(489..>2194)
                     /locus_tag="VEA63_08290"
     CDS             complement(489..>2194)
                     /locus_tag="VEA63_08290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009267 - cellular response to starvation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="carbon starvation CstA family protein"
                     /protein_id="HYD84039.1"
                     /translation="LPGLLWMLIGATLGGAVHDSIVLFCSIRRGGKSLGQMVRDEVGP
                     FAGVLALISTAAIMVIILAVLALVVVRALAESPWGLFTILATIPVAMIMGVGMRFTRI
                     SVVAISVFGVAGLLLSVVGGQWIHGTILEGWLTLDGRTLAIGIMAYGLLASVLPVWLL
                     LAPRDYLSSFMKIGAVALLAIAIVVLAPELKMPALTRFIDGTGPVFAGAVFPFCFITI
                     ACAAISGFHAIIASGTTPKIMARETDIRFVAYGAMVTEMLVGVMALIAACVMEPGEYF
                     AINLRGEPAAVTAAVTEMGFPVTVAQMETLAADVGEKTMIGRTGGAPTFAVGMAHMFA
                     GVLGSKGALALWYHFAIMFEALFILTTIDAGTRVGRFLMQDLLGLVWKPLRHTDSALA
                     SWLASIAFVAMWGFFLYQGVVDPLGGINSLWPIFGVANQLLAVIALALGTTVLLKMGR
                     RRYLWVPLLPLTWLLAVTMTAGWQKIFSADPRLGFLSAASRYHEQIASGGSAEQLAQW
                     GHLATSQYVNATVTGTFLVLVLLIVLTSAHVWWRLLSGRTAPALREEVAVRADFARQP
                     N"
BASE COUNT          467 a          827 c          584 g          316 t
ORIGIN      
        1 tccggcgccg ccgcatccac cgagccatcc gccgcccgca agtccgtggt gaacgccgat
       61 ggcagttccg gcaaccgtcc ggtgcgcgaa tcgtagacca gcaccgccgc ttccgcgcgc
      121 tcctctgccg gcgcgttctc cgccggcgct gcgtcgacaa aataaaccgc cggccacagc
      181 gacgccgcgt tcgcaaaatc gttcgggtat ttcgcggaaa atttcccgtc cgcgatcgcc
      241 acctgcgctg tcaccgtttt tccaataccg tctgtgaccc gcagccagac gcgtttgcct
      301 gtgcgctcgt ccgcgctgcc ctcaatgcga aactcacgcg cctccgccaa atcgcggaac
      361 ggcgtcaccg caaccgtctc ccgcaacgag accgctggtt tgcgtgcatt cgcgagcgcc
      421 ggcgccgcaa tcaacagtga cgctccaagc agcagcaaaa gagaggtcct tcgcatggtg
      481 accgcaaatc agttgggctg ccgtgcgaaa tccgcgcgga ccgcgacctc ttcgcgcaac
      541 gccggcgccg tgcgtcccga gagcaaccgc caccagacat gcgccgaggt cagcacgatc
      601 aacagcacga gcacgagaaa cgtgccggtc accgtcgcgt tgacatactg gctcgtcgcc
      661 agatgccccc attgcgcgag ctgttcggcc gagccgccgc tcgcgatctg ttcatggtag
      721 cgcgacgcgg cgctcagaaa tccgagccgc ggatcggcgc tgaaaatttt ctgccagccc
      781 gccgtcatcg tgacggcgag cagccacgta agcggcagca gcggcaccca gagataacgg
      841 cggcggccca tcttgagcag caccgtcgtg ccgagcgcca gcgcgatcac cgcgagcaac
      901 tggttcgcca cgccgaagat cggccagagc gagttgatgc cgcccagcgg atcgacgacg
      961 ccttgataaa gaaaaaagcc ccacatcgcc acgaacgcga tgctcgccag ccaactggcc
     1021 aacgccgaat cggtgtgacg cagcggcttc cacaccaacc ccaataaatc ctgcatcagg
     1081 aagcgcccca cccgcgtgcc tgcatcgatc gtcgtcagga taaacagcgc ctcgaacatg
     1141 atcgcaaaat gataccagag cgcgagcgcc cccttgctgc ccagcacgcc cgcgaacatg
     1201 tgcgccatgc ccacggcgaa cgtcggcgct ccgcccgtcc ggccgatcat ggtcttctct
     1261 ccgacatctg ccgccagcgt ctccatctgc gccaccgtca caggaaaccc catctccgtc
     1321 accgctgccg tcaccgccgc cggctcgccg cggagattga tcgcaaaata ttcgccgggc
     1381 tccatcacgc acgccgcgat caacgccatg actccgacca gcatctccgt caccatcgcg
     1441 ccatacgcga cgaagcggat gtcggtttcc cgcgccatga tcttcggcgt cgtgcccgac
     1501 gcgatgatcg catgaaaacc cgagatcgcc gcacatgcga tcgtgatgaa acaaaagggg
     1561 aacaccgcgc ccgcgaacac cggccccgtg ccgtcgatga aacgcgtcaa tgccggcatc
     1621 ttcaactccg gcgccagcac gacgatcgcg atcgcgagca acgccaccgc gccgatcttc
     1681 atgaaactgc tgagatagtc gcgcggcgcc agcagcagcc acaccggcag cacgcttgcc
     1741 agcaacccat aagccatgat cccgatcgcg agcgttcgcc catcgagcgt cagccagcct
     1801 tccaagatcg tgccgtgtat ccattgaccc cctacgacgc tgagcaacaa acccgcgacg
     1861 ccaaaaaccg aaatcgccac cacgcttatc cgcgtgaacc gcatccccac gcccatgatc
     1921 atcgcgaccg gaatcgtcgc cagaatcgtg aacagccccc acgggctttc cgccagtgcc
     1981 cgcaccacca ccagcgcgag caccgcaaga atgatcacca tgatcgccgc cgtgctgatc
     2041 aacgccagca ccccggcaaa cggcccgact tcgtcgcgga ccatctggcc gagcgacttc
     2101 ccgccgcggc ggatcgagca aaacaacacg atcgaatcat gcaccgcgcc gccgagcgtc
     2161 gcgccaatca gcatccataa caaccccggc aaaa
//
LOCUS       DASPLT010000796         1670 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_33542, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000796 DASPLT010000000
VERSION     DASPLT010000796.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1670)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1670)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1670
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_33542"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1631)
                     /locus_tag="VEA63_08295"
     CDS             complement(<1..1631)
                     /locus_tag="VEA63_08295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009509497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HYD84040.1"
                     /translation="MIAIVIMIAGALSITQLPISRYPTIAPPTVSVSAVYPGASAQVV
                     EDSVTQIIEQNMKALDGLLYMTSTSQSNGAAQITLTFSSGTNADIAQVQVQNKLQLAT
                     ALLPQIVQQQGVSVTKTGEGFLQVIGFVSEDGSMSRDDISDFITTNVVDPLSRVPGVG
                     STQVFGARYAMRIWLDPNKLDAYRLSTGEVIAAIQGQNQQVAVGQLGGTPAVPGQMLN
                     ATINARGRLQTPEQFREIVLRSGEDGAVLKLGDVARVEMGGENYAIIGRFNGQPASGV
                     AVSLATGANALATSTAVSALIEQLKPTFPEGLKTVIPFDTTPFVRVAIWEVVKTLLEA
                     IVLVFIVMFLFLQNFRATLIPTIAVPVVLLGTFAVLAALGFSINMLTMFAMVLAIGLL
                     VDDAIVVVENVERLMTEEGLSPLDATRKSMDQITGALVGIGLVLSAVFVPMAFLGGST
                     GVIYRQFSATIVAAMALSVLVAIVLTPALCATMLKPIQQGHHVREKGFFGWFNRLFDR
                     GNARYQGAVRGILSRGKRFFAVFVAMVALMVVLFLR"
BASE COUNT          335 a          572 c          483 g          280 t
ORIGIN      
        1 cgcaggaaca gcacgaccat cagcgcgacc atcgcgacaa acaccgcgaa gaagcgtttt
       61 ccccgggaga ggatgccgcg gaccgcgcct tggtagcgtg cgttgccgcg gtcgaagagt
      121 cggttgaacc agccgaagaa gcccttctcg cgcacatgat gcccctgctg gatcggcttc
      181 agcattgtgg cgcacagcgc gggggtgagc acgatggcga ccagcaccga gagagccatg
      241 gcggcaacaa tggtggcgga gaattgccgg tagatcacgc ccgtcgagcc gccgagaaac
      301 gccatcggca cgaacaccgc cgacaacacc aaaccgatgc cgaccagcgc gcccgtgatc
      361 tggtccatcg atttccgcgt tgcgtcgagc ggtgacaatc cttcctccgt catcaaccgc
      421 tcgacgtttt ccacaacgac gatcgcgtcg tccacgagca ggccgatcgc cagcaccatg
      481 gcgaacatcg tcagcatgtt gatcgagaaa cccagcgccg ccagcaccgc aaacgtgccg
      541 agcaagacca ccggcaccgc aatcgtcgga atgagcgtcg cgcggaaatt ctgcaaaaac
      601 agaaacatga cgatgaatac caggacgatc gcctcgagca gcgtcttcac cacttcccaa
      661 atcgccaccc gcacgaacgg cgtcgtgtcg aacggaatca ccgtttttag cccctccgga
      721 aacgtcggct tcaactgctc gatcaacgcg ctcaccgccg tcgacgtcgc gagcgcgttc
      781 gctcccgtcg ccagcgacac cgccacgccg ctcgccggct ggccgttgaa ccgcccgatg
      841 atcgcgtagt tctcgccgcc catctcgacg cgcgccacgt cgcccagctt caacaccgcg
      901 ccatcctcac cgctgcgcag cacgatctcg cgaaactgct ccggagtttg caggcgaccg
      961 cgcgcgttga tcgtcgcgtt gagcatctgc ccgggcaccg ccggcgtgcc acccaactgg
     1021 cccacggcaa cttgctggtt ctgaccctgg atcgccgcaa tcacctcgcc cgtcgaaagc
     1081 cggtaagcat cgagtttatt cgggtcgagc cagatgcgca tcgcgtagcg cgcgccgaac
     1141 acctgcgtgc tgccgacgcc cggcacgcgc gacaacggat ccacgacgtt cgtcgtgatg
     1201 aagtccgaaa tatcgtcgcg cgacatgctg ccgtcctccg acacgaagcc gatcacctgc
     1261 agaaagcctt cgccggtctt cgtcacgctc acgccttgtt gctgcacgat ctgcggcagc
     1321 aaggccgtcg cgagttgcag tttgttctgc acctgcactt gcgcgatgtc cgcgttcgtg
     1381 ccgctcgaga acgtcagcgt gatttgcgcc gcgccgttcg actggctcgt cgacgtcatg
     1441 tagagcagcc cgtcgagggc tttcatgttt tgctcgatga tctgcgtcac cgaatcttcg
     1501 acgacctgcg ccgaggcgcc gggataaacc gcgctgaccg acacggtcgg cggcgcgatc
     1561 gtgggataac gcgagatcgg gagctgcgtg atcgacagcg ctccggcgat catgatgacg
     1621 attgcgatca cccatgcgaa gatgggccgg tcgataaaaa agcgtgccat
//
LOCUS       DASPLT010000797         2034 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_296790, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000797 DASPLT010000000
VERSION     DASPLT010000797.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 2034)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2034)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2034
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_296790"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(584..1681)
                     /locus_tag="VEA63_08300"
     CDS             complement(584..1681)
                     /locus_tag="VEA63_08300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007358728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HYD84041.1"
                     /translation="MSLQIKPRASCAFDQISLGEVMLRLDPGEGRIRTAREFKVWEGG
                     GEYNTSRGLRKCFGYKTAVATAFVDNEVGHLVEDFIMQGGVATDFIKWREDDGIGRTV
                     RNGLNFTERGFGVRGAVGNPDRGNTAASQLKPGDFDWDHIFGKLGVRWFHTGGIFAAL
                     SESAAALTVEAVKAANKHGTIVSYDLNYRPSLWKTIGGLKKAQEVNREIAKYVDVMIG
                     NEEDFTASLGFEVKGVDHNISAIETDAFKAMIETAVKEFKNFKVAATTLRRVITATKN
                     DWSAILWHDGKFHESRKYPELEILDRVGGGDSFASGVQFGFMEFNDAQKAVEYGAAHG
                     ALASTTPGDTSMATRKEVEKQIGGGGARVVR"
     gene            complement(1810..>2034)
                     /locus_tag="VEA63_08305"
     CDS             complement(1810..>2034)
                     /locus_tag="VEA63_08305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012377061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate dehydrogenase"
                     /protein_id="HYD84042.1"
                     /translation="GRNQIGNVKAVIEDILGHGNEGCMLPGQLEAQAAARCAKNGGLL
                     FSEAEINAFSEIAGEIGKRAWVLTDLKVAE"
BASE COUNT          378 a          672 c          571 g          413 t
ORIGIN      
        1 ttcgctgcaa cgcagcgaaa agagatcggg aaatcggcgg gaaaggtgtg acgacagccg
       61 gggggcattg ggtcctccag caatcgtatc ctctgatcca atactttagt ttttaatgga
      121 caaacccgcc ggcgataccc cgagtcgcca gtcacctgcc ctcgtttggc aactgcctgc
      181 gtcgaaccac ctccggtcgg gtattccact cgctgagggg atttggctgc gcagtggcgg
      241 tatgccggac cggcacagga cgtgtaacgt ctgcgagtaa gcgcttcaca cggtcatctt
      301 cgcgcgaatg ccggccaccc taattaaaac tcccgcgctt cgccgatcag gcggtgcacg
      361 cacccgcccg ctcgcatatt cgcccttcga aaatcagcgc aaattagcgg ttcaaaactc
      421 cgtcctccat cgtcaaacgt ccgccgatca aagctcagcg gcgatgaatc atggagccgc
      481 gacgccctcg ccgcgtttct ctcttatgaa cgcggcgagg gcgccgcagc tccatcgaac
      541 agccgactgc gcaccctcgc cgaagatctg atttatccca aactcagcgc accacgcgcg
      601 cgccgccgcc gccgatctgc ttctcgactt ccttgcgcgt cgccatcgac gtgtcgcccg
      661 gcgtcgtcga cgccagcgcg ccgtgcgccg cgccatactc gaccgccttc tgcgcgtcgt
      721 tgaactccat gaacccgaac tgcacgccgc tcgcaaacga gtcgccgccg ccgacgcggt
      781 cgaggatctc cagctccgga tacttgcggc tctcgtggaa ttttccgtcg tgccagagaa
      841 tcgcgctcca gtcgttcttc gtcgcggtga tcacccggcg caacgtcgtc gcggccacct
      901 tgaagttttt gaactccttc accgccgtct cgatcatcgc cttgaaggcg tccgtctcga
      961 tcgcgctgat gttgtgatcg acgcctttca cctcgaagcc gagtgacgcc gtgaagtcct
     1021 cctcgttacc gatcatcacg tcgacatact tcgcgatctc gcggttgacc tcctgcgctt
     1081 ttttcaagcc accaatcgtc ttccacaacg acggcctgta gttgagatcg taggacacga
     1141 tcgtgccgtg cttgttggct gctttaaccg cttcgaccgt caacgccgcc gcggattccg
     1201 acaacgccgc gaaaattccg ccggtgtgaa accagcgcac gccgagcttg ccgaaaatat
     1261 gatcccagtc gaaatcgccg ggcttcaact gcgacgccgc ggtgttgccg cgatccggat
     1321 tgccgaccgc gccgcgcacg ccgaagccgc gctcggtaaa attcagcccg ttgcgcaccg
     1381 tgcggccaat gccgtcgtct tcacgccact tgatgaagtc cgttgcgacg ccgccctgca
     1441 tgatgaagtc ctcgacgaga tggccgactt cgttgtcgac gaacgcggtg gcgacggcgg
     1501 tcttgtagcc gaagcatttc cgaaggccgc gcgacgtgtt gtattcgccg ccgccctccc
     1561 agactttgaa ttcgcgcgcg gtgcgaatcc ggccttcgcc gggatcgagg cgaagcatga
     1621 cttcgccgag cgaaatttgg tcaaaggcgc acgaggcgcg gggcttgatt tgaaggctca
     1681 tgatagaaaa tagctgagag ctgagaggct agagctgaga gcaagaaagt ggatgactga
     1741 gcgagaacga gtgagagatc gtagattggc tctggactct cagctcttag ctctcagctc
     1801 gcggcccgat cactccgcga ccttgaggtc ggtcaggacc caggcgcgtt taccgatctc
     1861 tccggcgatc tcgctgaacg cgttgatctc cgcctcggag aacaacagcc cgccgttctt
     1921 cgcgcaacgc gccgccgcct gcgcttcaag ttggcccggg agcatgcagc cttcgttgcc
     1981 gtgaccgagg atgtcctcga tcactgcttt cacgttgccg atctgattgc ggcc
//
LOCUS       DASPLT010000798         2065 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_33556, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000798 DASPLT010000000
VERSION     DASPLT010000798.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 2065)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2065)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2065
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_33556"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..530
                     /locus_tag="VEA63_08310"
     CDS             <1..530
                     /locus_tag="VEA63_08310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007363864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HYD84043.1"
                     /translation="VLEEYQRAKKRGAKIYAEIAGYHVNSDASDYVLPNPTRQAECVR
                     ASISRAGLQPGDIHIVNTHATATPLGDVQECEAIRAVFGENCPDTHINNTKSYIGHAM
                     GAAGALELAGNLPSFDDTTVHPTINVDDLDPACAIPGLVMNQPRKVAKVDTILNNSFG
                     MLGINSTLIVKRFAP"
     gene            550..813
                     /locus_tag="VEA63_08315"
     CDS             550..813
                     /locus_tag="VEA63_08315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HYD84044.1"
                     /translation="MTKDECKKVVLDIIADIAPDEDLSNVKPDVRLRDQLQLDSMDFL
                     DIVMELRKRHGIEVPEKDYLQLASLDSSAEYLTPKFNALTPKS"
     gene            1157..>2065
                     /locus_tag="VEA63_08320"
     CDS             1157..>2065
                     /locus_tag="VEA63_08320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009512261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HYD84045.1"
                     /translation="MNTTSHYDVAIIGAGMSGLAAGIRLAHFGKRVCIFERHNVVGGL
                     NSFYSIGGRKYDVGLHAMTNFVRPGVKGTPLTKLLRQLRIDRDEFALCEQKQSRVAFG
                     PRGESSLRFTNDFTVFESEVAAKFPAQIDGFRRLVALVRSYDDVSLDAAPESAREVVR
                     RHIGDPLLEDMLFCPVMYYGSATERDMEFGQFTIMFKALFLEGFARPFEGVRVILRVL
                     LEKYRAAGGERRMKCGVRRILAHGGKATALVLDNGEEVTADHVISSIGAPETEGLMAE
                     EGSRVDGRLRVEPDVSSGFSADAAPAN"
BASE COUNT          399 a          578 c          675 g          413 t
ORIGIN      
        1 tggtgctgga agaataccag cgcgccaaaa agcgcggcgc gaagatttac gccgagatcg
       61 ccggctatca tgtgaactcc gacgcgagcg attacgtttt gccgaatccc acgcgccagg
      121 ccgaatgcgt gcgcgcgtcg atctcacggg cgggtttgca gccgggcgac atccatatcg
      181 tgaacacgca tgccaccgcc acgccgctcg gcgacgtgca ggaatgcgag gcgatccgcg
      241 cggtgttcgg cgagaattgc cctgacacgc acatcaacaa cacgaagagc tacatcggcc
      301 atgcgatggg cgcggcggga gcactggagc tggcgggaaa tctcccgtcc ttcgatgaca
      361 ccacggtgca tccgacgatc aacgtcgacg atctcgatcc ggcgtgcgcg atccccggac
      421 ttgtgatgaa ccagccgcgg aaagttgcga aagtggacac catcctgaac aattccttcg
      481 ggatgctcgg gataaattcc acgttgattg ttaagcggtt tgccccctaa tcctgcgccc
      541 cctcgccaaa tgaccaaaga cgaatgcaag aaggtggtgc tcgacatcat cgccgacatc
      601 gcccccgacg aagacctcag caacgtgaag ccggacgtgc gtctgcgcga ccagttgcaa
      661 ctcgattcga tggacttcct cgacatcgtg atggagctcc gcaagcgcca cggcatcgag
      721 gtgccggaga aggactacct gcaactcgcc tcgctcgaca gctcggcgga gtatctgacg
      781 ccgaagttca acgcgctgac gccgaagagt tgaggcgcgc gcgtgttttg aagcggtcag
      841 gccgggcttc tgcccggcct tgctttttct gggagagggc gatccgggtt taaccacgaa
      901 tagacacgaa gggacacgaa ttccgaatct gttattcgtg cgtattcgtg gttcaataac
      961 tccgattttt ttaaccacgg atgaacaccg atagacacgg attaggcctc ggacttcgca
     1021 agctctgctg tgaatccgtg tccatccgcg ttcatccgcg gttgttgttt ccgatccgat
     1081 tccggatcga tgattcgtgt gagttcgtgt ccattcgtgg ttcaacgact cggacttcga
     1141 attttccgac ttgggcgtga atacgacttc gcattacgac gtcgcgatta tcggtgcggg
     1201 catgtcgggg ctcgcggcgg gcatccggct cgcgcacttc gggaagcgcg tgtgcatttt
     1261 cgagcggcac aacgtcgtcg gcgggctgaa tagcttttat agtatcggag ggcggaaata
     1321 cgacgtcggc ttgcacgcga tgacgaactt cgtgcggcct ggcgtgaagg gcacgccgct
     1381 gacgaaattg ctgcggcaac tgcggatcga tcgcgacgag ttcgcgttgt gcgaacagaa
     1441 gcagtcgcgc gtggcgtttg gtccgcgcgg tgagagctcg ctgcggttca cgaacgattt
     1501 cacggtgttc gagagcgaag tcgcggcaaa gtttcccgca cagatcgacg gttttcgccg
     1561 gctcgtggcg ctcgtgcgca gctatgacga cgtgtcgctc gacgcggcac cggagtcggc
     1621 gcgcgaagtt gtgcggcggc acatcggcga tccgttgctc gaggacatgt tgttttgtcc
     1681 ggtgatgtat tacggcagcg cgaccgagcg cgacatggag ttcgggcagt tcacgatcat
     1741 gttcaaggcg ctgtttctcg aaggcttcgc gcggccgttc gaaggcgtgc gcgtgatcct
     1801 gcgcgtcctg ctggagaaat atcgcgcggc cggcggcgag cggcggatga aatgcggcgt
     1861 gcggcggatc ctcgcgcacg gcggcaaggc aacggcgctg gtgctcgata acggcgagga
     1921 ggtgacggcg gatcatgtga tctcgtcgat cggtgcgccg gagaccgaag gtttgatggc
     1981 ggaggaaggc tcgagggttg acggaaggtt gagggtggag cccgacgtct cgtcgggctt
     2041 ttcggcggat gccgcgccgg caaac
//
LOCUS       DASPLT010000799         2110 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_111439, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000799 DASPLT010000000
VERSION     DASPLT010000799.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 2110)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2110)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2110
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_111439"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..904)
                     /locus_tag="VEA63_08325"
     CDS             complement(<1..904)
                     /locus_tag="VEA63_08325"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HYD84046.1"
                     /translation="MVHLNHYPMPNLPIPRTPEELLAQMVSFETVNPEFGGPAGGEAR
                     LATHIESLARSWNLDTKRCPVNDSGAFNLLVTVKSSASASSDAEWLLFESHLDTVSAE
                     GMSVPAFELTTRGDRLHGRGACDTKGSGAAMLWALKDYAATSSRRRNAGVIFVVDEEA
                     GMTGAQAFAARELREFENLRGVIVGEPTRLRPVIAHNGALRWRSTTRGVAAHSSDPSK
                     GRSAISAMLEVVAALEEKFIPLATAAHPLTGRAAASINVIRGGRAVNVIPDRCEILCD
                     RRLVPGENVAVVVAARDAALAGLAV"
     gene            complement(1054..>2110)
                     /locus_tag="VEA63_08330"
     CDS             complement(1054..>2110)
                     /locus_tag="VEA63_08330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD84047.1"
                     /translation="QRALTRYYVAAGSGGLAVGVHSTQFEIRQPQHALFRPVLELAAE
                     TVRAELTRMDHTHAAETQVGTPLRGVRGPLGEPPLPSELSTAPRDFALIAGICGQTPQ
                     ALAEAELARSLGYHAGLVSMAAFATEPEDAILAHIATVADAIPVIGFYLQPAVGGRIF
                     SYAFWRQFAEIPNVVGIKIAPFNRYQTLDVIRAVVAAGRDDLALYTGNDDTIVADLLT
                     PFTFPHEGRPVTRRIVGGLLGHWGVWTRAAVELFRQAQSAARGPQLDAAWLGRAAAVT
                     DMNAAVFDAANRFHGCIPGILEVLRRQGLVETTHCLNPNEVLSPGQSEELDRVTAAYP
                     DLTDDIFVAEHRDRWLR"
BASE COUNT          365 a          555 c          836 g          354 t
ORIGIN      
        1 cgacagcgag accggcgagc gcggcgtcgc gagcggcgac aacgacggcg acgttttcgc
       61 cgggaacgag acggcggtcg cagaggattt cgcagcgatc cggaatgacg ttcaccgcgc
      121 ggccgccgcg gatgacattg atgctggcgg cggcgcgacc agtgagcggg tgcgcggcgg
      181 tggcgagcgg gatgaatttt tcctcaaggg cggcgacgac ctcgagcatc gcggaaatcg
      241 ctgagcggcc tttgctcgga tcggaggagt gggcagcgac gccgcgtgtg gtggagcgcc
      301 agcgaagcgc gccgttgtgc gcgatcaccg gacgcaggcg cgtcggttcg ccgacgatga
      361 cgccgcgaag gttttcgaac tcgcgcagct cgcgagcggc gaaggcttgg gcgccggtca
      421 tgccggcttc ctcgtcgacg acgaaaatga cgccggcgtt gcgccggcgc gacgacgtgg
      481 cggcgtagtc cttgagcgcc cagagcatcg cggcgccgga gcccttcgtg tcgcaagcgc
      541 cgcggccgtg gagtcggtcg ccgcgggtgg tgagttcgaa cgccggcacg ctcatgcctt
      601 cggcgctgac ggtgtcgaga tggctttcga agagcagcca ttcggcgtcg ctcgatgcgg
      661 aagcggagga tttgaccgtg acgagcaggt tgaacgcgcc cgaatcgttt acggggcagc
      721 gtttcgtatc gaggttccaa ctacgagcga gcgattcgat gtgagtggcg agacgggctt
      781 cgccaccggc ggggccgccg aactccggat tgacggtttc gaacgagacc atctgggcga
      841 ggagttcctc gggggtgcgt gggatcggca gattgggcat tggataatga ttcaaatgaa
      901 ccacgaatgg acacgaattg acacgaatgg aaatgctgtc gaacagaggc tcgtgagcat
      961 gtcaggtgtc gaaggaatgg gcttggagac ttcggtgcgg ggcggttggt attcgtgtga
     1021 attcgtgtcc attcgtggtt ctgccaacgg cgattagcgc agccagcggt cgcggtgttc
     1081 ggcgacgaat atgtcgtcgg ttaaatctgg atacgcggcg gtgacgcgat cgagctcctc
     1141 ggactggccg ggggagagga cttcgtttgg gttgaggcaa tgcgtggttt caacgagacc
     1201 ctggcgacgg aggacttcca gaatgccggg aatgcagccg tggaagcggt ttgcggcgtc
     1261 gaagacggcg gcgttcatgt cggtgacggc ggcggcgcga ccgagccagg cggcgtcgag
     1321 ttgcgggccg cgcgcggcgc tttgggcctg acggaaaagc tcgacggcgg cacgtgtcca
     1381 cacgccccag tgaccgagca gaccgccgac gatgcggcgg gtgacggggc ggccttcgtg
     1441 aggaaaggtg aacggcgtga ggagatcggc gacaatggtg tcgtcgttgc cggtgtagag
     1501 cgcgagatcg tcgcggccgg cggcgacgac ggcgcggatg acgtcgagcg tttgatagcg
     1561 gttgaaaggc gcgatcttga tgccgacgac gttggggatt tcggcgaatt gccgccagaa
     1621 ggcatagctg aaaatgcgtc cgccgacggc gggttgaaga tagaagccaa ttactggaat
     1681 cgcgtcggcg acggtggcga tgtgcgcgag aatggcgtct tcgggttcgg tggcgaaggc
     1741 ggccatgctg acgaggcccg cgtgataacc gagggagcgg gcgagttcgg cttcggcgag
     1801 ggcctggggc gtctggccgc agatgccggc gatgagggca aaatcgcgtg gggcggtgct
     1861 cagctcggaa ggtaggggcg gctctccgag cggtccgcgg acacctcgga gaggcgtccc
     1921 tacctgtgtc tccgcggcgt gggtgtgatc catccgggtc agctcggcgc gaacggtttc
     1981 ggcggcgagc tcgaggacgg ggcggaagag ggcgtgttga ggctggcgaa tttcgaactg
     2041 cgtggagtgg acgccgacgg cgagaccgcc ggagccggcg gcgacgtagt agcgcgtgag
     2101 ggcgcgctgg
//
LOCUS       DASPLT010000800         1442 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_10380, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000800 DASPLT010000000
VERSION     DASPLT010000800.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1442)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1442)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1442
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10380"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..641)
                     /locus_tag="VEA63_08335"
     CDS             complement(<1..641)
                     /locus_tag="VEA63_08335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009511927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HYD84048.1"
                     /translation="MPAFKIPSPPPRLRLSGIHKRFGATLALSGVDLTVAAGEVHALI
                     GENGAGKSTLMKILSGVHAPDAGTIELDGRPFAPRAPQDARRAGVVMVNQELAIAPHL
                     TVVENIVLGAEPACGLLRHAEARRLASDALAQLGRPDIPLHARAGSLSIAAQQLIEIA
                     RALALGCRVLVLDEPTSSLSRSDTENLFQLIRRLRDQGESVVYISHFLEEVQA"
     gene            complement(878..>1442)
                     /locus_tag="VEA63_08340"
     CDS             complement(878..>1442)
                     /locus_tag="VEA63_08340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HYD84049.1"
                     /translation="VATDNYRGGQLGGEELARLLDGKGKVVLLRYAEGSASTTARENG
                     FLDVIKRHPGLELIVENRYAGATVSTAQDAAMNLLDKIREADGIFCVNESATQGMLLA
                     LRQTGLVGKKKFVGFDTSPALMAALNKGEIAALVAQNPTRMGYLGVVTAVKHLRGEKV
                     ETEIDTGCELVTKANQNDPAIKAVLGK"
BASE COUNT          255 a          408 c          491 g          288 t
ORIGIN      
        1 atcgcctgca cctcttccag aaagtgcgaa atgtagacga cggactcgcc ttggtcgcgg
       61 agacggcgaa tgagttgaaa caaattctcc gtatccgaac ggctgagact gctcgtcggt
      121 tcatcgagga cgagcacgcg acaaccgagc gcgagcgcgc gcgcgatctc gatcaactgc
      181 tgcgcggcga tgctcaacga gccggcgcgc gcgtgcagcg gaatgtcggg ccggccgagt
      241 tgcgcaagcg catcgctggc gagtcggcgc gcttcggcgt ggcggagcag accgcacgca
      301 ggctcggcgc cgaggacgat gttttcgacg acggtgagat gcggcgcgat cgcgagctct
      361 tgattcacca tgacgacgcc ggcgcggcgg gcgtcctgcg gcgcgcgcgg cgcgaaaggc
      421 cgtccgtcga gctcgatcgt gccggcatcg ggggcgtgaa cgccggagag gattttcatc
      481 agcgtgctct tgcccgcgcc gttctcgccg atcagcgcat ggacctcgcc cgcggccacg
      541 gtgagatcga cgcccgagag cgcgagcgtg gcgccgaaac gtttgtggat gccggaaaga
      601 cgaaggcggg gagggggaga gggaatcttg aaagcgggca tcctagtttg acgacggagg
      661 tcagagtttt tgaaccgcta atcggcgctg atttctcgga ggccgatggt gatggcgaat
      721 gaatgcgtgc ttcgcctaat cggcgaagcg cggacgtttc atcgggatcg ggcggggtta
      781 cattagcgga gattagcgat gattagcggt taactgtatt tttgcttcgg atatctcccg
      841 ctagattttc gatgcccgat tatcagtttt cccgatctta cttccccagc acggctttga
      901 tcgcggggtc gttctgattg gctttcgtca cgagttcgca gccggtgtcg atttcggttt
      961 ccaccttttc gccgcgaagg tgtttcacgg ccgtgacgac gccgagatag cccatgcgcg
     1021 tcgggttttg cgcgaccagc gcggcgattt cgcccttgtt caatgcagcc atcagcgccg
     1081 gcgatgtgtc gaaaccgacg aatttctttt ttccgacgag gccggtctgg cggagcgcga
     1141 gcagcatgcc ttgggtggcg gactcgttga cgcagaaaat gccgtcggct tcgcgaatct
     1201 tgtcgaggag gttcatcgcg gcatcctgcg cggtcgaaac ggtcgcaccg gcatagcgat
     1261 tttcgacgat gagctcgaga ccggggtggc gcttgatgac atcgagaaag ccgttctcgc
     1321 gcgcagtcgt gctcgcagaa ccctcggcat agcggagaag aacgaccttg cccttgccgt
     1381 cgaggagacg cgcgagttcc tcgccgccga gctggccgcc gcgatagttg tcggtggcga
     1441 cg
//
LOCUS       DASPLT010000801         1701 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_33622, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000801 DASPLT010000000
VERSION     DASPLT010000801.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1701)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1701)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1701
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_33622"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..983)
                     /locus_tag="VEA63_08345"
     CDS             complement(<1..983)
                     /locus_tag="VEA63_08345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD84050.1"
                     /translation="MHNSSLIRPHFVWFVVVALLALVAARAQENETDDATLAAKVAKG
                     RSQYLAGENEMAEETFREVERADPGNPEADEFLKRIARDRLARGELHRERTRAQLLNE
                     VTQSWERPGVLRGSGDDAQTKSPTAPLVQKLNEIVLPTVSFTRAEIAQVVAALSAASE
                     EFDATSVLPKGVNIVLLDPANRAPTVTLTLRNAPLKRVLDFVAESIGYQYEVQADAVV
                     LRPGGETTMLDTEFFPVTRATVLRMTGNTLAISPSAARAEAFSSAGASNDAGPASNAA
                     EGEAMKNFLQQAGVNFEAVAGSSLAYDGSALIVTQTSRNVERIRNILARYN"
     gene            complement(991..>1701)
                     /locus_tag="VEA63_08350"
     CDS             complement(991..>1701)
                     /locus_tag="VEA63_08350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD84051.1"
                     /translation="SERSAWHAPSPQPRGPGWVYELFTPPEIYRDSRTRDFSVAAPVA
                     LPLPPATELAIAAPSAAFPLELVEVKRALFRLQLIGFVGDEGRGSGLFENAATTEIFV
                     IRGKCRRPELDLTIEDFAVRRSPMPLAESMTTQRLVAHARVRDERTGAVVELNSLERR
                     HTDEAVAVVTLRDAPLPRVVREGDAIEAEGAVYKIRKIQLHPPTLQVSRESRGGGSDE
                     QRTLTARDEKHGASAPAS"
BASE COUNT          300 a          635 c          449 g          317 t
ORIGIN      
        1 tcattgtaac gcgccaggat gttgcggatc cgttccacgt tgcgcgacgt ctgcgtcacg
       61 atcagcgccg agccgtcata agccaggctc gaaccggcca ccgcttcgaa attcacgccc
      121 gcttgctgca gaaaattctt catcgcttcg ccctccgccg cattcgaagc cggaccagca
      181 tcgttgctcg cgcccgccga cgagaatgcc tccgccctcg cggcactcgg cgagatcgcc
      241 agggtgtttc ccgtcatccg caagaccgtc gcgcgcgtca ctggaaaaaa ttccgtgtcc
      301 aacatcgtcg tctcgccgcc cggtcgcagc accaccgcat ccgcctgcac ttcgtattgg
      361 tagccgatcg actcagccac gaaatcgagc acgcgcttca atggcgcatt tcgcagcgtg
      421 agcgtcaccg tcggcgcgcg attcgccgga tcgagcagca cgatgttcac gcctttcggc
      481 aacacgctcg tcgcgtcgaa ttcctccgat gccgcgctca acgctgcgac cacctgggcg
      541 atctccgctc gcgtgaaact cacggtcggc agcacaatct cgttcagctt ctgcaccaac
      601 ggcgccgtcg gcgatttcgt ctgcgcgtcg tccccgcttc cccgcaaaac gccggggcgc
      661 tcccagctct gcgtcacctc gttcaacaac tgcgcgcgcg tccgctcgcg gtgaagttcg
      721 ccccgggcca ggcgatcccg cgcgatccgc ttcaaaaatt catcggcctc gggattcccc
      781 ggatcagccc gttcaacctc gcgaaaggtt tcctccgcca tctcattttc gccggcgaga
      841 tactggcttc gtcccttcgc cactttcgcc gccagcgtcg catcgtccgt ctcgttctcc
      901 tgcgctctcg ctgctaccag cgcgagcagc gccacgacga caaaccagac gaaatgcggc
      961 cgaatcagcg atgagttgtg catgcgaaaa tcaagacgcc ggtgccgacg caccgtgctt
     1021 ttcgtcgcgt gcagtcaacg tccgttgttc gtcgctgccg cctccacgcg attcccggct
     1081 cacctgcaaa gtcggcggat gcagttgaat tttccgtatt ttatacaccg caccctccgc
     1141 ctcgatcgca tcgccttcgc gcaccactcg gggcagcggc gcatcccgca gcgtcaccac
     1201 cgcgaccgct tcgtccgtgt gccggcgttc aagactgttc aactccacga ctgcaccggt
     1261 gcgttcgtcc cgcacgcgcg catgcgccac gagccgctgc gtcgtcatgc tctccgccag
     1321 cggcatcggc gagcgccgca ccgcgaaatc ttcgatcgtc agatcaagct ccggccgccg
     1381 gcattttccg cgaatcacaa aaatctccgt cgtcgccgca ttctcgaaca acccggaacc
     1441 acgcccttcg tcgccgacga atccgatcag ttgcaaccga aacaacgccc gcttcacctc
     1501 caccaattcc agcggaaacg ccgccgacgg cgccgcgatc gcgagctcag tcgccggtgg
     1561 caaaggtaac gccacgggtg ccgcgacact aaagtcacgt gtgcgcgaat cgcgatagat
     1621 ctccggcggc gtgaacaact cgtagaccca tcccggcccg cgaggctgag gacttggcgc
     1681 gtgccacgcc gaccgttcgc t
//
LOCUS       DASPLT010000802         1775 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_33629, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000802 DASPLT010000000
VERSION     DASPLT010000802.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1775)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1775)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1775
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_33629"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..196)
                     /locus_tag="VEA63_08355"
     CDS             complement(<1..196)
                     /locus_tag="VEA63_08355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD84052.1"
                     /translation="MNLRAILRVGVAARGLALLLALAAACVAAEEKNTREIDGETWAT
                     TELVRERFEDDRWRKRWKLEG"
     gene            complement(193..1533)
                     /locus_tag="VEA63_08360"
     CDS             complement(193..1533)
                     /locus_tag="VEA63_08360"
                     /inference="COORDINATES: protein motif:HMM:NF017280.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylxylan esterase"
                     /protein_id="HYD84053.1"
                     /translation="MRLSFFTRLSACLALAFAWLPLNAVAIEAVPDRVSVIDLQVAPD
                     RADWLYQPGEKVTFRVSVTADGLPLAGSAIEYRVGPEMMPAASREANVSAEGLLIDGG
                     TLDHPGFIRCVVTATIDGRSYRSLATAGFAPEKIAPTQEEPTDFDAFWNSSKEELAKI
                     PLEPRLELIPEASKGAINVYHVSFRTWSRTEAVRNAGRIYGILCEPKAPGRYPAILRV
                     PSAGVRPYSGQRELAERGAITLEIGIHGIPVTHPLEIYDQMRTGALDGYPNYNLDDRE
                     FYYFRRVYLGCLRANDFLTTREAWDGRNLLVFGASQGGQLTTVTAALDPRVTAAAAVV
                     PAYCDVSGYLHDRAGGWPHMMRGADSRHRTPAKIATTQYYDTVNFAKRVRAPIFVALG
                     YNDETCPPTSIFSAYNSLTAPKRLLVAPEMTHAVFPEVDERVRAWLFEQIKRTP"
     gene            complement(1565..>1775)
                     /locus_tag="VEA63_08365"
     CDS             complement(1565..>1775)
                     /locus_tag="VEA63_08365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD84054.1"
                     /translation="MNLDTALNLHSGVLSVLIESPSHSFAGTNRAGEVAPHSADQLVD
                     AQLVAYEAGLTYLADHGGRTKWALK"
BASE COUNT          342 a          581 c          537 g          315 t
ORIGIN      
        1 tgccctccag cttccaacgc ttgcgccagc gatcatcctc gaaccgctca cggaccagct
       61 cggtcgtagc ccaagtttcg ccatcgatct cgcgcgtgtt cttttcctcc gccgcgacgc
      121 acgccgctgc caaagccagc aacaaggcaa gtcctcgcgc ggccacacca acccggagaa
      181 tcgcgcggag gttcatggcg tccgcttgat ctgctcgaac agccaggctc tcacgcgttc
      241 atcgacttca gggaaaaccg cgtgagtcat ttcgggcgcg acgagcaaac gcttcggcgc
      301 ggtgagcgag ttataagcgg agaagatcga cgtcggcgga cacgtctcgt cgttgtaacc
      361 gagcgcaacg aagatcggcg cgcgcacgcg tttcgcgaaa ttcaccgtgt cgtaatactg
      421 cgtcgtcgcg atcttcgccg gcgtgcgatg acggctgtcg gcgccgcgca tcatatgcgg
      481 ccatccgccc gcgcgatcgt gcaggtagcc ggatacatcg cagtaagccg gcacgaccgc
      541 cgccgccgcg gtcacgcgcg gatcgagcgc ggcggtcacc gttgtgagtt gcccgccctg
      601 gctcgctccg aaaacgagga ggttgcgacc atcccacgct tcgcgcgtgg tgaggaaatc
      661 gttcgcgcgc agacagccga gatacacgcg gcggaaataa tagaactccc gatcgtcgag
      721 attatagttc ggataaccgt ccaacgctcc ggtgcgcatc tggtcgtaaa tctcaagcgg
      781 gtgcgtcacc ggaattccgt gaatcccgat ctcgagcgtg atcgcgccgc gctccgccaa
      841 ctcgcgctgc cccgagtagg gtcgcacgcc ggcactcggc acgcgcaaaa tcgccggata
      901 acggccgggc gctttcggct cgcagaggat gccgtaaatt cgccccgcat tgcgcacggc
      961 ttccgtccgg ctccacgtgc gaaaactcac gtgataaaca ttgatggctc ctttgctggc
     1021 ttcgggaatc aattcgagtc gcggctcgag cggaatcttc gcgagctcct ccttgctgga
     1081 gttccagaac gcgtcgaagt ccgtcggttc ttcctgcgtc ggcgcgattt tttccggcgc
     1141 aaaacccgcc gtcgccagac ttcgataaga gcgcccgtca atcgtagccg tgacaacgca
     1201 gcgaatgaaa cccggatgat cgagcgtgcc gccgtcgatg agcagacctt ccgctgaaac
     1261 gttcgcttcg cggctcgccg ccggcatcat ctcggggccc acgcgatact cgatcgctga
     1321 gccggcgaga ggaagtccgt cggcggtgac gctcacgcga aacgtcacct tttcgcccgg
     1381 ctggtagagc cagtcggcgc gatcgggcgc gacctgcaga tcgatgaccg acacgcggtc
     1441 gggcaccgct tcgatcgcaa ccgcattgag cggcaaccag gcgaacgcga gcgccagaca
     1501 agcgctcagg cgggtgaaga atgaaaggcg caagatcaca gcggtagatt ttcgccgggc
     1561 gaatttactt cagcgcccac ttcgtgcggc cgccgtggtc ggcgagataa gtcaggcccg
     1621 cttcgtaagc caccaattgc gcgtccacca gttgatcagc cgagtgcggc gcgacttcgc
     1681 ccgcgcgatt cgttccggca aaactgtgcg acggcgactc gatcaacacc gacagcacgc
     1741 cgctgtgcag attgagcgcg gtgtcgagat tcatg
//
LOCUS       DASPLT010000803         1850 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_111509, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000803 DASPLT010000000
VERSION     DASPLT010000803.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1850)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1850)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1850
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_111509"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..238
                     /locus_tag="VEA63_08370"
     CDS             <1..238
                     /locus_tag="VEA63_08370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009513564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD84055.1"
                     /translation="VVWPWARATGEWAEYFETKANYTMMFHGRAALPWRFTENRPVAL
                     VFAFVPEERPLTFEVRDARVVTIERDRGAEKLRR"
     gene            complement(621..>1850)
                     /locus_tag="VEA63_08375"
     CDS             complement(621..>1850)
                     /locus_tag="VEA63_08375"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HYD84056.1"
                     /translation="LGLVYFAVIGVQIANPAWNYMLSTAGWWVEPVAHIPWLPHGVAG
                     TPFAKMNAWRTLMIHGGIWLAVCALWIGITRRRSAQVLLVAIAINGVALAVVAILQRL
                     TESDRLLFFWPAPTPYFVASFIYKNHAGAYLNLILALCLGLAWWHADRAEQRMAKSHP
                     GLLFLFLAVFVGTALLFTYAVAATALGLAFVLTVAIGYVTLRIFHAKHGGSSRSATLL
                     TGGLCAVFLGLCAYSLNIEKVSRKFGRVSDEEYTSSITYRQLATKATFDMGRDELWLG
                     HGIGGYRYVFPRFQQAYPEIFTSRKRRLYWEHAHNDYVEYFAESGVLGLGLLALGSVF
                     VIVGMRRAGVFSQPPGLIVAGGPLLVVVYAAADFPTHNPAVLTTACAILVLTTRWVGL
                     DRPKHARTAGNAENLKS"
BASE COUNT          393 a          482 c          671 g          304 t
ORIGIN      
        1 tgtggtctgg ccgtgggcgc gggcgacggg cgagtgggcg gagtatttcg aaacgaaggc
       61 gaactacacg atgatgtttc acggtcgcgc ggcgctgcca tggcggttta ccgaaaatag
      121 gccggtggcg ctggtgttcg catttgtgcc ggaggagcgg ccgctgacgt tcgaggtgcg
      181 tgatgcgcgg gtggtgacga ttgagcgcga tcgaggggct gagaaactga gacgctgaaa
      241 agctgagacg ctgaaaactg aaaactaacg gagcgcggca gcgcgtagat gtaggactgc
      301 cgcttgcggc atgccgtgtc agccggagca attcggtagg gaaccacgga cgaagacgga
      361 tggacccgga tcgaaaatct tttccatacc gcataatacc gtaaccgcta attatcgctc
      421 atctccgcta atggaatgat ggcgaggccc gacgcaaacc tgagaccggc ggtcggtaaa
      481 ccgtgggaag tagcgtgtgg acggcagaca gtggtccgca gacggaagcg aggcgtgccc
      541 atttggccgg cgaaggagca aagacggagc gaggttgaag ctgagtcgtc gtgatcggcc
      601 tcgattcagg tttcagcttc tcaggatttc aggttttcag cgtttcccgc cgtgcgcgcg
      661 tgcttggggc ggtcgaggcc gacccagcga gttgtcagca cgaggatggc gcaagcggtc
      721 gtcagcacgg cgggattgtg cgtcggaaaa tccgctgccg cgtagaccac gacgaggagc
      781 gggccgccgg cgacgatgag ccccgggggc tgcgaaaaca cgcccgcgcg acgcatgccg
      841 acgatcacga agacggatcc gagagcgagc aggcccaagc cgagaacgcc gctttcggcg
      901 aaatattcca catagtcgtt gtgagcgtgt tcccagtaga ggcgacgttt tcggctggtg
      961 aagatttccg ggtaagcctg ttggaagcgc ggaaacacgt agcgatagcc gccgatcccg
     1021 tggccaagcc agagctcgtc gcggcccatg tcgaaggtgg ctttggtggc gagctgtcgg
     1081 taagtgatcg aactggtgta ttcctcgtcg gagacgcgtc cgaatttgcg ggatactttt
     1141 tcgatgttga gcgaataagc gcagagccca aggaagacgg cgcagaggcc gccggtgagc
     1201 agcgtagcgc tgcgtgacga gccaccgtgt ttggcgtgga agatacgcag cgtgacgtag
     1261 ccgatggcga cggtcaggac aaaagcgagg ccgagcgcgg tggcggcgac tgcatacgtg
     1321 aagagcaggg cggtgccgac gaaaacggcg aggaagagaa acagcaggcc ggggtgggat
     1381 ttcgccatgc gttgctcggc ccgatcggcg tgccaccagg caaggccgag acacagggcg
     1441 agaatgaggt tcaggtaggc gccggcgtgg tttttgtaga tgaacgacgc cacgaagtag
     1501 ggggttgggg caggccaaaa aaagagcagc cgatcgctct cggtgaggcg ctgcaggatg
     1561 gcgacgacgg cgagcgcaac gccattgatc gcgatcgcaa ccagcaggac ttgtgcgctg
     1621 cggcgacggg tgatgccgat ccagagcgcg cagaccgcga gccagatgcc tccgtgaatc
     1681 atcagcgtgc gccaggcgtt catcttggcg aacggcgttc cggcgacgcc gtgcggcagc
     1741 caggggatgt gcgcgacggg ctccacccac cacccggcgg tgctgagcat gtagttccag
     1801 gcggggttcg cgatctgaac cccgatgacg gcgaagtaaa cgagtccaag
//
LOCUS       DASPLT010000804         1098 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_111519, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000804 DASPLT010000000
VERSION     DASPLT010000804.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1098)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1098)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1098
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_111519"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..298
                     /locus_tag="VEA63_08380"
     CDS             <1..298
                     /locus_tag="VEA63_08380"
                     /inference="COORDINATES: protein motif:HMM:NF019982.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HYD84057.1"
                     /translation="PVTPQTPPEPPAGAQTPILMLSGEFDPATPSQWADAAIQRFTNA
                     RHVIVPGSGHVIDGMSGVDTCFDPLVLAFLDSADPASLDTTCINEMRAPAFATE"
     gene            301..>1098
                     /locus_tag="VEA63_08385"
     CDS             301..>1098
                     /locus_tag="VEA63_08385"
                     /inference="COORDINATES: protein motif:HMM:NF013781.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HYD84058.1"
                     /translation="MSFQHAPDPPWIFRTYAGHSTPVESNKLYRANLAKGQTGLSVAF
                     DLPTQTGYDSDHILARGEVGKVGVPVGHLGDMRALFDGIPLEQMNTSMTINAPAAWLL
                     ALYVSLAEEQGADPKKLTGTTQNDILKEYLSRGTYIFPPAPSLKLTADTIAWSLDAIP
                     KWNPMNVCSYHLQEAGATPEQELAFALAAAIAVLDEVKARGEVEPERFALVFSRISFF
                     VNAGLRFVTELCKMRAFVQLWDEIGRERYGVDDEKARRFRYGVQVNSL"
BASE COUNT          210 a          384 c          311 g          193 t
ORIGIN      
        1 gccggtgacg ccgcaaacgc cgccagagcc gccagccggc gcgcagacgc ccatactgat
       61 gctgtccggc gagttcgatc cggcgacgcc atcgcaatgg gccgatgcgg ccattcaacg
      121 attcaccaac gcgcgccacg tcatcgttcc tggcagcggg catgtcatcg acggcatgtc
      181 cggcgtcgat acatgcttcg acccactcgt cctcgctttc ctcgacagcg ccgatcccgc
      241 ttcgctcgac accacttgca tcaacgagat gcgtgcgccc gcgttcgcca cggagtagaa
      301 gtgagcttcc agcacgcccc cgacccgccc tggattttcc gcacctatgc cggccattcg
      361 accccggtcg agtccaacaa gctctaccgc gccaatctgg ccaagggaca aactgggctc
      421 tccgtcgcct tcgatctgcc gacccagacc ggctacgaca gcgaccacat cctcgcccgc
      481 ggcgaagtcg gcaaggtcgg cgtgccggtg ggccacctcg gcgacatgcg cgcactgttc
      541 gacggtatcc cgctcgaaca aatgaacacg tcgatgacga tcaatgcgcc ggcggcttgg
      601 ctgctggcgc tttacgtttc actcgccgaa gaacaaggcg ccgatccgaa aaagctcacc
      661 ggcaccacgc agaacgatat cctcaaagaa tatctctcgc gcggcactta catcttcccg
      721 ccggcgccat ctctgaagct caccgcggac accatcgcct ggagcctcga cgcgattccc
      781 aaatggaatc cgatgaacgt ctgctcttat cacctgcaag aagccggcgc gacgccggag
      841 caggaactcg ccttcgccct cgccgcggcg atcgcggtgc tcgatgaagt gaaagcgcgc
      901 ggcgaggtgg agccggagcg tttcgcgctg gtgttcagcc gcatctcttt tttcgtgaac
      961 gccggcctgc gcttcgtcac cgaactctgc aagatgcggg cgttcgtaca attgtgggac
     1021 gagatcggcc gcgaacgcta tggcgtcgat gacgaaaaag cgcgccgttt ccgctatggc
     1081 gtgcaggtca actcgctg
//
LOCUS       DASPLT010000805         1075 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_255769, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000805 DASPLT010000000
VERSION     DASPLT010000805.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1075)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1075)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1075
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_255769"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..443
                     /locus_tag="VEA63_08390"
     CDS             <1..443
                     /locus_tag="VEA63_08390"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HYD84059.1"
                     /translation="LAALVGTRPDFHLDPEDELAGKISALPDRLILAYPVISAVNECA
                     HCRSFERLLGPNPSDEMRRQLSPELHVSAQTPPTFIFHAADDKNVSVENSIAFARACW
                     AKGLPAELHVFPRGGHGRQFAYAADVSPRWRELLREWLAAWELK"
     gene            528..>1075
                     /locus_tag="VEA63_08395"
     CDS             528..>1075
                     /locus_tag="VEA63_08395"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HYD84060.1"
                     /translation="MPSFGFNPANLTARKPLGLCVALFAFLLPRITSADTSPPPHSPN
                     LVVILADDMGYSDLGCYGQTRYTTPRLDQLAREGVRFTDFYASQPVCSASRASLLTGV
                     YANRLGIHGALGSSSRHGIHSDETTLGELCQRAGYATAIFGKWHLGHLPAFSPLRHGF
                     DQFYGIPYSNDMWPHHPTSGQK"
BASE COUNT          190 a          363 c          300 g          222 t
ORIGIN      
        1 atctggcggc gttggttggc acccgtccgg attttcatct cgatccagag gacgagttgg
       61 ctggcaagat cagcgcgctg cccgaccgct tgatcctcgc ttatccggtg atttcggcgg
      121 tgaacgagtg cgcacattgc cgctcgttcg agcgtttgtt ggggccaaac ccctcggatg
      181 aaatgcgccg gcagctatcg ccggagttgc acgtctccgc gcaaacgcca cccacgttta
      241 tcttccacgc cgcggacgac aagaatgtgt cggtggagaa cagcatcgcg tttgctcgcg
      301 cctgctgggc gaaaggtctg ccagcggagt tgcacgtctt cccacgcggt ggccatggcc
      361 ggcagttcgc ctatgcggcg gacgtatcgc cccgctggcg cgaacttctg cgcgagtggc
      421 ttgccgcgtg ggagctaaag taaatggtcg cggatcgcag ctccactccg gcgaagcagc
      481 agcgtaaaat aactcaatcc atctgcgtca cgtccccaac cacgttcatg ccctctttcg
      541 gattcaaccc ggctaatctc accgcccgca aaccgctcgg tctttgcgtc gcattgttcg
      601 ctttcctgct tccgcgaatc acctccgccg acacaagtcc gccgccgcac tcgcccaacc
      661 tcgtggtgat cctcgcggac gacatgggct acagcgatct cgggtgctac ggtcaaacgc
      721 gctacaccac cccccgcctc gaccaactcg cgcgcgaagg cgtgcgcttc accgattttt
      781 atgcgagcca gcccgtatgc tccgcctcgc gcgcgtcgtt gctgacgggc gtgtatgcga
      841 accgcctcgg cattcacggc gcgctgggct cgagttcgcg ccacggtatc cattcagatg
      901 agacaacgct cggagaactc tgccaacgcg ccggctacgc gaccgcgatt ttcgggaaat
      961 ggcacctcgg acaccttcct gcgttttcac cgctgcggca tggtttcgat cagttttacg
     1021 gcattcctta ctcgaacgac atgtggccgc atcacccgac cagcggccaa aaatt
//
LOCUS       DASPLT010000806         1046 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_255773, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000806 DASPLT010000000
VERSION     DASPLT010000806.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1046)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1046)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1046
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_255773"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..987
                     /locus_tag="VEA63_08400"
     CDS             <1..987
                     /locus_tag="VEA63_08400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009508007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatine deiminase family protein"
                     /protein_id="HYD84061.1"
                     /translation="RQEAVWLSWPHNRASWPGQFRPVPAAFASIVAAISRFEKVRINC
                     AANLQVRAKRFCAKAGADMSKVTFYDHPTNDAWCRDHGPIFVKNDATGEVAITDWAYN
                     AWGDKYPPYDLDNEIPPSIARKLKLRRFVNDMILEGGSIDVNGKGLLLTSEQCLLNEN
                     RNPQLSREQIETNLKDYLGVKTVLWVGEGIIGDDTDGHIDDITRFYREDALVTCVETN
                     KRDPNHKILEDVMERLKSFRTPKGKKFEIATLPMPKPFGFRGQMVPASYANFLVINGA
                     VLVPNFRQPKRDAEANEVLGACFPEREVIPIDCYHIIWGLGTLHCLSQQQPA"
BASE COUNT          218 a          270 c          359 g          199 t
ORIGIN      
        1 cggcaggaag ccgtgtggtt gtcgtggccg cataatcgcg cgtcgtggcc cgggcagttt
       61 cgcccggtgc cggcggcgtt cgcgagcatc gtcgcggcga tcagccgatt cgagaaagtg
      121 cggatcaact gcgcggcgaa cctgcaggtg cgcgcgaaac gtttctgcgc gaaggcgggc
      181 gcggacatgt cgaaggtgac gttctacgat catccgacga acgatgcgtg gtgccgcgat
      241 cacggtccga ttttcgtgaa gaacgatgcg acgggcgaag tcgcgatcac cgactgggcc
      301 tacaacgcgt ggggcgacaa gtatccgcct tacgacctgg acaacgagat cccgccgagc
      361 atcgcgcgca agctgaagct gcggcggttc gtgaacgaca tgattctcga aggcggctcg
      421 atcgacgtga acgggaaggg gctgctgctg acgagcgagc agtgtttgtt gaacgagaac
      481 cggaatccgc agctttcgcg cgaacagatc gagacgaacc tgaaggatta tctcggcgtg
      541 aagacggtgc tctgggtggg cgaaggtatc atcggtgatg atacggacgg gcacatcgac
      601 gacatcacgc ggttttatcg ggaggatgcg ttggtgacgt gcgtggaaac gaacaagcgc
      661 gatccaaatc acaagattct cgaggacgtg atggagcggc tgaaaagctt ccgcacgccg
      721 aaggggaaga agttcgagat cgcgacgctg ccgatgccga agccgtttgg gtttcgcggg
      781 cagatggtgc cggcgagcta cgcgaatttt ctggtgatca acggcgcggt gttggtgccg
      841 aattttcggc agccgaaacg cgatgcggaa gcgaacgaag tgctgggcgc gtgtttccct
      901 gagcgcgagg tgatcccgat cgattgctat cacatcatct ggggactcgg gacgctgcat
      961 tgtttgtcgc agcagcagcc ggcgtgagct tttttcttca catgaacatg ctggccactc
     1021 gacgaaccga ttctacttca gcaggg
//
LOCUS       DASPLT010000807         2235 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_133248, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000807 DASPLT010000000
VERSION     DASPLT010000807.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 2235)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2235)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2235
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_133248"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..500
                     /locus_tag="VEA63_08405"
     CDS             <1..500
                     /locus_tag="VEA63_08405"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HYD84062.1"
                     /translation="GTAPDGTTLFTDVQGGSQLSEGFEVDLLFTPIDDLQVMLAFSRC
                     DAIYEQYPSNPALDRAPLVATPDRTFSLWGKYFFRSGSLEGFAVAGGISHVGSASHVA
                     NNPLVRTPAYTTIDLTLGYRFELFGRKCSADLSVKNLVNERYYASASSWGFPRHVIFS
                     LGARF"
     gene            complement(545..985)
                     /locus_tag="VEA63_08410"
     CDS             complement(545..985)
                     /locus_tag="VEA63_08410"
                     /inference="COORDINATES: protein motif:HMM:NF044606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YetF domain-containing protein"
                     /protein_id="HYD84063.1"
                     /translation="METVVRVVLIYVFILVGLRLLGKREFSQLSAIDLVTLLLVPEIV
                     SQAITRDDFSLTNAFVAVATLFCLVFFTSALAHRFRRVERFINGAPALLVARGKILFA
                     NCDRERISAEEIFSEMHKNGLDSLEAVRWAILEVDGRISIVPEK"
     gene            complement(988..1506)
                     /locus_tag="VEA63_08415"
     CDS             complement(988..1506)
                     /locus_tag="VEA63_08415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD84064.1"
                     /translation="MSSRTERSTIPPPAPPSKRRIRFPVAQIVGLGMFSVLPILAIFD
                     RFGTGETRLTASSDDVSVAVEYSPRVRYGLPTQVRIEARAAGGRDAGPVTVRVEREFV
                     DRFAQLSLLPEPSEMEASSYTFVLPASDVGGAILIELEPEQYGPARGSVIASTAGGGA
                     EAKVELSAFIFP"
     gene            complement(1757..2059)
                     /locus_tag="VEA63_08420"
     CDS             complement(1757..2059)
                     /locus_tag="VEA63_08420"
                     /inference="COORDINATES: protein motif:HMM:TIGR00741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044238 - primary metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPF/RaiA family ribosome-associated protein"
                     /protein_id="HYD84065.1"
                     /translation="MKAAIVARAERLFRHEPHILMLRVDVERDHKRRGRMFVAKGKVE
                     IAGPDITASTASGDAYLAIGLLIDKLDRMLRKRATALRRKRHAGDIREHSTNEVLV"
     gene            2015..>2235
                     /locus_tag="VEA63_08425"
     CDS             2015..>2235
                     /locus_tag="VEA63_08425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD84066.1"
                     /translation="MAKQSFGSRHDCGFHCIGEFDVDSTDNQFRTLVNRQGWDHGFFL
                     SGAAQARGTGASFAGFRNLPRRDDVDDNA"
BASE COUNT          442 a          756 c          632 g          405 t
ORIGIN      
        1 gcgggaccgc tcccgatgga acgacgctct tcaccgacgt ccaaggcggc tcgcagctca
       61 gcgaagggtt cgaagtggac ctgctcttta ctccgatcga cgacctgcag gtgatgctcg
      121 ccttcagccg ttgcgacgcg atctatgagc aatatccgtc gaaccctgcg ctggatcgtg
      181 cgccgctcgt cgccacgccc gatcgcacct tcagcctgtg gggaaaatat tttttccgct
      241 cgggcagcct cgaaggattc gccgtcgccg gcgggatcag ccatgtcggt agcgcgagcc
      301 acgtcgccaa caaccccctc gtgcgaacgc ccgcctacac gacgatcgat ctcacgctcg
      361 gttaccgttt cgagcttttc ggccgcaaat gctccgccga cctctccgtg aagaacctcg
      421 tcaacgaacg ctactatgcg tccgcctcga gctggggttt tccgcggcac gtgatctttt
      481 cgctcggcgc gcgattctaa acgcacctcg gccgccctcg cgcgtcaggc ccttccgccc
      541 gcgctcactt ttccggcacg atcgaaatcc ggccgtcgac ttccaggatc gcccaacgca
      601 ccgcttcgag cgaatcgagg ccgttcttgt gcatctcgct gaaaatttcc tccgccgaga
      661 tccgctctcg atcgcagttc gcaaacagga ttttcccccg cgcaaccaag agcgccggcg
      721 cgccgttgat gaaacgttcc acccgccgaa aacgatgcgc cagcgccgag gtgaagaaca
      781 ccaggcagaa cagcgtcgcg acggcgacga atgcgttcgt cagcgaaaag tcatcgcgcg
      841 tgatcgcctg cgacacgatc tcgggcacca gcagcaacgt cacgagatcg atcgccgaca
      901 actggctgaa ctcacgcttc ccgaggaggc gcagccccac caggatgaag acatatatca
      961 aaacgacgcg gacgacggtt tccatgttca ggggaaaata aacgcgctca actccacctt
     1021 cgcttccgca ccaccgcctg ccgtcgatgc gatcacgctg cctcgcgcgg ggccatactg
     1081 ctccggctcc aactcgatca aaatcgctcc tccgacgtcg gacgccggca gaacaaacgt
     1141 gtaggagctc gcctccattt cactgggctc gggcaacagc gagagctgtg cgaaccgatc
     1201 gacgaactca cgctccacgc gcaccgtcac cggccccgca tcgcgcccgc ctgccgcgcg
     1261 tgcttcgatc ctcacctgcg tcggcaagcc gtagcgcacg cgaggcgaat actcgaccgc
     1321 cacgctcaca tcatcgctcg acgccgtgag ccgcgtctcg cccgtgccga agcggtcgaa
     1381 gatcgcgaga atcggcagca ccgaaaacat cccgaggccc acaatctgtg cgactgggaa
     1441 acgtatccga cgcttcgatg gcggcgcagg aggaggaatc gtgcttcgtt cggttcggga
     1501 cgacatgaga agggcaataa gcagcacggc tgctgtgctt ccgatggacc ggacgcgccc
     1561 catcacgttc ccagcactga cgtcaggcga ggaaacgcgc gaccgccgag gtggtgtgga
     1621 tcacatgaag tgacgttgat gcgcaatccg cgattcaacg tcacctcgga gagcgcattg
     1681 aatccgaacg aaagcgtaag ctgccatgaa tcggccgcag cgccgccgcg gccgattcac
     1741 ccacataaac gcatgctcac accagaactt cgttcgtcga gtgctcgcgg atgtcgccag
     1801 cgtgacgctt gcgccgcagg gccgtggcac gcttgcgcaa catccggtcg agcttgtcga
     1861 tcaagagccc gattgccaga tacgcatcgc cgctcgccgt cgacgccgtg atgtccggcc
     1921 cggcgatctc gaccttgcct ttcgcaacga acatgcgtcc gcggcgcttg tgatcgcgct
     1981 ccacatcgac gcgcaacatc aggatgtgcg gctcgtggcg aaacagtcgt tcggctctcg
     2041 ccacgattgc ggctttcatt gcatcggtga gttcgacgtg gattccacgg acaatcagtt
     2101 tcgcactctc gtgaatcggc agggatggga tcatggcttc tttctttctg gtgcagcgca
     2161 ggcgcgtggc accggcgctt catttgcagg ttttcgaaat ttaccgcgtc gcgacgacgt
     2221 ggacgacaat gcaag
//
LOCUS       DASPLT010000808         2037 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_111570, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000808 DASPLT010000000
VERSION     DASPLT010000808.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 2037)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2037)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2037
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_111570"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1261
                     /locus_tag="VEA63_08430"
     CDS             <1..1261
                     /locus_tag="VEA63_08430"
                     /inference="COORDINATES: protein motif:HMM:NF019222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1552 domain-containing protein"
                     /protein_id="HYD84067.1"
                     /translation="ALSLPFLDSMLPGFARAASAAAASPLTPGGSPRRMFGICNNLGL
                     LPELFFPTGNGRDYVASPYLKLLDAHRNDFTVLNGVSHPNVDGGHPADISFLTAAPHP
                     ASSSFRNTISLDQYIAERIGILTRFPSLTLAVNTNTRSLSWTGSGVAIPPEEKASDVF
                     KQLFLQGTQEEIEAQIRDLDTGRSILDAVADQAKDLQWRVGAHDRTRLDQYFTSVRDL
                     ESRMQASRGWETKPKPVVQASVPVDPASPAQYMAKVKVMYDLAKLAFETDSTRSITLM
                     LNSVGTPVVEIRGATITEDYHNLSHHGKAEDKLAQLKVLDEWQMKLLAGLMTDLKSVR
                     EGEETLLDRTMVLYGSNLGDANAHSTTNMPTLFAGGGFRHGQHLVFDRQQNYPLPNLF
                     VSMLQRMGIEEEKFASSTGTMRGLEMA"
     gene            1363..1872
                     /locus_tag="VEA63_08435"
     CDS             1363..1872
                     /locus_tag="VEA63_08435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD84068.1"
                     /translation="MAGAVLLLVANVAHAEGVIDFEQAEVGKRTTEWVEQGVVIKLAH
                     APTQTKAPGRVVFFEHHGTGRRGVLSAMAMEPIPVEMSFPRAVSAVTLALWGSTGCAA
                     RVEALDAAGNVVDHAEVEPVPRRKAPGEPVPVFELTVRGKEIARVRFSGPRAGEYLAA
                     DAVRFTFAE"
BASE COUNT          359 a          506 c          797 g          375 t
ORIGIN      
        1 ggcgttgtcg ctgccgtttc tcgactcgat gctgccggga ttcgcgcggg cggcgagtgc
       61 ggcggcggct tcgccgttga caccgggcgg ctcgccgcgg cggatgtttg ggatctgcaa
      121 taatctcgga ctgctgccgg agttgttttt tccgacgggg aatggacgcg actacgtggc
      181 gtcgccgtat ttgaaattat tggacgcgca ccggaacgat ttcacggtgc tcaacggcgt
      241 gtcgcatccg aacgtggatg gcgggcatcc ggcggatatc agttttctga cggcggcgcc
      301 gcatcctgcg agcagttcgt ttcggaacac gatctcgctc gatcaataca tcgcggagcg
      361 aatcggcatc ttgacgcgtt tcccgtcgct gacgttggcg gtgaacacga atacgcggag
      421 cctctcgtgg acggggagcg gggtggcgat tccgccggag gaaaaggcgt cggacgtgtt
      481 caagcagttg tttctccaag gaacgcagga ggagatcgag gcgcagatcc gagatttgga
      541 tacggggcgg agcatcctcg atgcggtggc ggaccaggcg aaggatttgc agtggagagt
      601 cggcgcgcat gatcgcacgc ggctggatca gtatttcacg agcgtgcggg atttggagag
      661 ccggatgcag gcgtcgcgcg gctgggagac aaagccgaag ccggtggtgc aggcgagcgt
      721 gccggtcgat ccggcgagtc cggcgcagta tatggcgaag gtgaaagtga tgtatgatct
      781 ggcgaagctc gcgttcgaga cggattcgac gcgatcgatc acgctgatgt tgaacagcgt
      841 cgggacgccg gtggtggaga tccgcggcgc cacgatcacg gaggattatc acaacctgtc
      901 gcaccatgga aaagcggagg acaaactcgc ccagttgaag gtgctcgacg agtggcagat
      961 gaagctgctc gcgggattga tgacggattt gaagagcgtg cgcgaaggcg aggagacgct
     1021 gctggatcgg acgatggtgc tttacgggtc gaatctcggc gatgcgaacg cgcactcgac
     1081 gacgaacatg ccgacgctgt ttgcgggcgg cgggtttcgg catggccagc atctggtgtt
     1141 cgatcggcag cagaattatc cgctgccgaa tttgttcgtc tcgatgctgc agcggatggg
     1201 aatcgaggag gagaaattcg cgtcgtcgac ggggacgatg cggggattgg agatggcgtg
     1261 agggtgcttc ctgaaggtgg tcgtttatcc cgctgctcac gcagcgagcc acgtcggacg
     1321 acgcggcgga tcgcgtgcaa gcaggctccg gcctttcgag ccttggcggg cgcggtgttg
     1381 ctgctggtgg cgaatgtggc gcacgcggag ggggtgatcg acttcgagca ggcggaggtg
     1441 gggaagcgga cgacggagtg ggtggagcag ggcgtcgtga tcaagctcgc gcatgcgccg
     1501 acgcagacga aggcgccggg gcgggtggtg tttttcgaac atcatggaac ggggcgtcgc
     1561 ggcgttttga gcgcgatggc gatggagccg attccggtgg agatgagttt tccgcgcgca
     1621 gtttcggcgg tgacgctggc gctgtgggga tcgacgggtt gcgcggcgcg agtcgaggcg
     1681 cttgatgcgg cgggcaatgt cgtcgatcac gcggaggtgg agccggtgcc gcgacgcaag
     1741 gcgccgggcg agccggtgcc ggtgttcgag ttgacggtgc gcggcaagga aatcgcgcgc
     1801 gtgcgattca gcgggccgcg cgcgggagag tatctcgcgg cggatgcggt gcggtttacg
     1861 ttcgcggagt gaggcaggcg cgagcgcgga aggaggcgag tgcgcaagcg cagaagaagg
     1921 cgggctgaag cccgccctac ttggccacga cgaagggggc gaagtagccg gattgggtgg
     1981 gcgggaaacg gcctttgatg cggacggcgc cgtcgacttc ttcctgctcc tggatgc
//
LOCUS       DASPLT010000809         3222 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_70021, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000809 DASPLT010000000
VERSION     DASPLT010000809.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 3222)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3222)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3222
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_70021"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..527
                     /locus_tag="VEA63_08440"
     CDS             <1..527
                     /locus_tag="VEA63_08440"
                     /inference="COORDINATES: protein motif:HMM:NF021505.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2231 domain-containing protein"
                     /protein_id="HYD84069.1"
                     /translation="PRAAVKLIDILQGKWLGHPLHPAIVHVPIGGWLAACVFDVIIAA
                     GWTQGGPSRLAFYCVVVGLIGAVLAVPPGIADWLSIKKGKPAHKLGVYHMLLNFAAAI
                     VWGVNLALRLSTSQPVNAMILATSILGTVLVLVSGYLGSLMVFDHGVSVARQSKKVWR
                     ERAARGGARLPDET"
     gene            538..1365
                     /locus_tag="VEA63_08445"
     CDS             538..1365
                     /locus_tag="VEA63_08445"
                     /inference="COORDINATES: protein motif:HMM:NF021505.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2231 domain-containing protein"
                     /protein_id="HYD84070.1"
                     /translation="MASLKNLAEGKPLRSPLHPAIVHLPIALFPIGVLLDFGSWMCPT
                     RDFYLVRGAFYCLVAGLATGLLAGVFGFVDYSDIRRDHPARKTATRHMVLNLVALALF
                     AVSAALRYDHLEAVRTAALPLWISVAALAVLSYSGYLGGHLVYSDGVGVGRHRREPPM
                     PDETIAARADAGGMASVAESSALAEGATLRVDVAGTIVALARVDGQVHAFQEFCTHRF
                     GPLSEGALRGCEVICPWHNSRFDIRTGRVTAGPAKVDLRTFRAEERDGRIWLEVPRA"
     gene            complement(1470..1949)
                     /gene="raiA"
                     /locus_tag="VEA63_08450"
     CDS             complement(1470..1949)
                     /gene="raiA"
                     /locus_tag="VEA63_08450"
                     /inference="COORDINATES: protein motif:HMM:TIGR00741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HYD84071.1"
                     /translation="MKKLPVGCPTLQVGGFPANDASDGGSGSGMKPEIPDDEDELLTK
                     LTIQGIHFDLTEAMQNIIREKFAVLLRHNDYIVRINVRLHQDQTMGTEHHYTATAQIE
                     IGGPDLVASAEGKDAYDLIDELVDKLDRQLGRRQGKRKEKRNRPHAPEIAANLPKVE"
     gene            2179..>3222
                     /locus_tag="VEA63_08455"
     CDS             2179..>3222
                     /locus_tag="VEA63_08455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006680774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HYD84072.1"
                     /translation="MLPLRWFLVLVGLFVANVSLHVWFSATNPAAAATTHNRLATAEF
                     LDAARAGKLSEGHIVYRTNALGLVDLNATLAESETTRPVHTTARLTDADLTLLREQRF
                     IEEDAATFAAAQHVTAAERTARIARGALAPLGILVALGAVVIIAQRYAGRFTAFSGQR
                     LRAVTSSVKFSAVAGCDEAKDEVHEVVEFLKDPARFSATGGRMPKGVLLVGPPGTGKT
                     MLAKAVAGEAKAHFYSLSGSDFVELYVGVGASRVRSLFKKARETAPSIIFIDEIDALG
                     RQRASGDASGAQQEHDQTLNALLVAMDGFDSDDAVVVFGATNRPDTMDRALLRPGRFD
                     RQVSVGLPDLAGRA"
BASE COUNT          510 a         1157 c          939 g          616 t
ORIGIN      
        1 aaccccgcgc cgccgtgaag ctcatcgaca tcctgcaagg caagtggctg ggccatcccc
       61 tgcaccccgc catcgtccac gtgccaattg gcggctggct cgcggcgtgt gtgttcgacg
      121 tcatcatcgc cgccggctgg acccagggcg gtccgagccg cctcgcgttt tactgcgtcg
      181 tcgtgggatt gattggcgcc gtgctcgccg ttcctcccgg gatcgcggac tggctttcga
      241 tcaagaaagg caaacccgcg cacaagctcg gcgtgtatca catgctgctc aacttcgccg
      301 ccgcgatcgt ctggggagta aacctcgcgc tgcgcctgtc cacgtctcaa ccggtcaacg
      361 ccatgatcct cgcgacatcg attctcggca ccgtgctcgt cctggtcagc gggtatctcg
      421 gctcgctgat ggttttcgat cacggcgtca gtgtcgcgcg gcagtcgaag aaagtctggc
      481 gcgagcgcgc cgctcgcggc ggcgcgcggc tgccggatga aacctgatcg ctccgccatg
      541 gcgtcgttga aaaatctcgc cgaaggcaaa ccgctgcgct cgccgctgca cccggcgatc
      601 gtgcacctgc cgattgcact ttttccgatc ggcgtcctgc tcgatttcgg aagctggatg
      661 tgcccgacgc gcgatttcta cctcgtgcgc ggcgcgttct actgcctcgt cgccggcctc
      721 gccaccggat tgctcgcggg tgtgttcggc ttcgtcgact acagcgatat ccggcgcgat
      781 cacccggcgc gaaaaaccgc cacacggcat atggtgctca acttggtcgc actcgcgctc
      841 ttcgccgtga gcgccgcgct gcgctacgat cacctcgagg cggtgcgcac cgccgccctg
      901 ccactgtgga tctccgtcgc cgctctcgcc gtgttatcat attctggata cctcggcggc
      961 catcttgtct acagcgacgg cgtcggcgtc ggtcgccacc ggcgggagcc cccgatgccg
     1021 gacgaaacca tcgcggcgcg cgccgacgcc ggaggaatgg cgagcgtcgc cgaatccagc
     1081 gcgctcgccg agggcgcgac gctgcgcgtg gacgtcgccg gcaccatcgt agcgctcgct
     1141 cgtgtcgacg gccaggttca cgcattccag gaattttgca cgcaccgctt cggcccgttg
     1201 tccgaaggcg cgctgcgcgg ctgcgaagtt atctgcccgt ggcataactc gcgcttcgac
     1261 atccgcaccg gccgcgttac cgccggcccg gcgaaggtcg acctccgcac cttccgcgcc
     1321 gaagagcgcg acggccggat ctggctcgaa gtccctcgcg cctgagcccg agcgtgcgaa
     1381 gaacgcaccc tcatggagcc gcggcgcccc cccccgccgc gttcttaatt tcctcgcgaa
     1441 cgcgacaagc gttcccggct agccaccgtt cactccacct tcggcaagtt cgccgcgatc
     1501 tccggcgcgt gcggccggtt gcgcttctcc ttgcgcttgc cctgtcggcg tccgagttgg
     1561 cggtcgagct tgtccaccag ttcgtcgatc aaatcgtagg cgtccttgcc ctccgccgac
     1621 gccaccaagt ccggcccgcc gatctcgatc tgcgccgtcg ccgtgtagtg atgctccgtg
     1681 cccatcgtct gatcctgatg caagcgcacg ttgatccgca cgatgtagtc gttgtgacgc
     1741 agcagcaccg caaacttctc gcgaatgatg ttctgcatcg cctccgtaag atcaaaatgg
     1801 ataccttgga tggtcagctt cgtgagcagt tcgtcctcgt cgtctggaat ctcgggtttc
     1861 atgccgctac cagacccacc gtcgctcgca tcgttcgcgg gaaaacctcc cacctgcagc
     1921 gtggggcagc cgacgggaag tttcttcaat tatttacgag aaagcttttt caacgatttg
     1981 cgcaaatcgc tcgcgcccgg gtgccatgag acgatccgcg caactcgcga tccgcaacca
     2041 agctgcgcgc caccgattca agcgacgcgt tttttcgaaa agcaaattgc gctcgcgtaa
     2101 acgcactcaa cgcgccgcgc gtcactcaac tccgacgttg cccgggaagt ttccatctga
     2161 cgcataggag gaatgcctat gttgcctctc cgttggttcc tcgtgctggt cggtctgttc
     2221 gtcgcgaatg tgtcgctgca cgtttggttt tccgccacga accccgcggc cgcagcgacg
     2281 acccacaacc gtctcgcgac cgccgaattc cttgacgccg ctcgcgccgg aaaactttcc
     2341 gaaggccaca tcgtttatcg caccaacgcg ctcggtctcg tcgacctgaa cgccacgctg
     2401 gccgagagcg aaacgacgcg tcccgttcac accaccgcgc gtctcaccga cgccgatctc
     2461 acgctgttgc gcgagcaacg tttcatcgag gaagacgccg ccacgtttgc cgcggcgcaa
     2521 cacgtcaccg ccgccgaacg caccgcccgc atcgcccgcg gcgcgctcgc gccgctcggc
     2581 attctcgtcg cgctcggcgc cgtcgtcatc atcgctcaac gctacgccgg tcgcttcacc
     2641 gcgttctccg gccaacggct gcgcgccgtg acctcgagcg tgaaattctc cgccgtcgct
     2701 ggctgcgacg aagcgaagga cgaggtccac gaggtcgtcg agttcttgaa agatcccgcg
     2761 cgttttagcg cgaccggcgg tcgcatgccg aaaggcgtcc tcctcgtcgg ccctccgggc
     2821 accggcaaaa ccatgctcgc gaaagccgtc gccggcgaag ccaaggcgca tttctacagc
     2881 ttgagcggtt ccgatttcgt cgagctctac gttggcgtcg gcgcctcgcg cgttcgttcg
     2941 ctcttcaaga aagcgcgcga gaccgcaccc agcatcatct tcatcgacga aatcgacgcg
     3001 ctcggtcgcc aacgcgcgtc cggcgacgcc agcggcgcgc aacaagaaca cgatcaaacc
     3061 ctgaacgcgc tcctcgtcgc gatggatggc ttcgattccg acgatgccgt ggtggttttc
     3121 ggcgcgacca accgccccga tacgatggat cgcgcgctgc ttcgccccgg ccgtttcgac
     3181 cgccaggtgt ccgtcggcct gcccgacctc gccggccgcg cc
//
LOCUS       DASPLT010000810         1136 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_214099, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000810 DASPLT010000000
VERSION     DASPLT010000810.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1136)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1136)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1136
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_214099"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(226..>1136)
                     /locus_tag="VEA63_08460"
     CDS             complement(226..>1136)
                     /locus_tag="VEA63_08460"
                     /inference="COORDINATES: protein motif:HMM:NF017658.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="filamentous hemagglutinin N-terminal
                     domain-containing protein"
                     /protein_id="HYD84073.1"
                     /translation="KAYLTPSGVPVVDLNTANGAGLSHNKFTHYNVPTQGLVLNNSVP
                     TQFSYPSQLVGSVTVNFNTKDAAKVILNEVVAPNATTIAGFTEIVGGKADLVVANPYG
                     IAVKGGGFINTDRVTLATGEPMIGPDGTLAGLRVVRGELSIAGNGLNATNVSLIDLLA
                     RTVKIDGQVNAQELIVGAGANDYDYASRQLSANHAPDAAPAYAVDSSVLGGMYANRIR
                     LVATDAGVGVRMLGDDAAPATSTQPAACNSRTNFPPNATSASPAPAALKSQAPHRRSA
                     RNAISRSTPKRAIFPSATVLSSPAAI"
BASE COUNT          181 a          314 c          420 g          221 t
ORIGIN      
        1 tgaggcttcg gttggctgcg ctggcatcgg ccccgctata aaaacgggcg ccattcgcga
       61 actgcgccga gccggtttgc acgttgaacg cactgccagc gccgtaggcg gaggcgctga
      121 ctttcgcgtc tcctgttgtc gcgacggtga gtgtcccgat tgcaaaacga ttcgcgctcg
      181 atggtgccgt ggtcgacgtg tcgacgagcg agccaccggc gatggtcaga tcgcggccgg
      241 cgatgagaga accgtcgctg agggaaatat cgctcgtttt ggcgtcgagc gtgagatcgc
      301 gtttcgcgct gagcgacgat gcggcgcctg cgatttcaag gccgccggtg ccggtgacgc
      361 ggacgtcgcg ttcggcggaa agtttgttcg cgagttgcac gcggccggct gcgtcgaagt
      421 cgcaggcgca gcgtcgtcgc cgagcatgcg cacgccgaca ccggcatccg tagcgaccaa
      481 acggatacga ttcgcataca tgccgccgag cacggacgag tcgacggcgt aggcgggcgc
      541 ggcgtcgggg gcatggttgg cggagagctg gcgggacgcg tagtcgtagt cgttggcacc
      601 ggcgccaacg atgagttcct gggcgttgac ctggccgtcg atcttcacgg tgcgggcgag
      661 aagatcgatg aggctgacgt tcgtcgcgtt gagcccgttg cccgcaatgc tgagttcgcc
      721 gcggacgacg cgcaggccgg cgagggtgcc gtcggggccg atcatgggct cgccggtggc
      781 gagcgtcacg cggtcggtgt tgatgaagcc gccgcctttt acggcgatgc cgtaaggatt
      841 ggcgacgacg aggtcggctt tgccgccgac gatctcggtg aaaccggcga tggtcgtagc
      901 gtttggtgca acgacctcgt tgaggatcac cttcgcggcg tctttggtgt tgaaattgac
      961 ggtgacgctg ccgacgagtt gcgatggata gctgaactgc gttggcaccg agttgttgag
     1021 cacgaggccc tgcgtcggca cattgtagtg cgtgaatttg ttatgcgaga gtccggcacc
     1081 gttcgcggtg ttgaggtcga cgacgggcac gcccgacggg gtcaggtaag ctttcg
//
LOCUS       DASPLT010000811         1764 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_296917, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000811 DASPLT010000000
VERSION     DASPLT010000811.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1764)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1764)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1764
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_296917"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..474
                     /locus_tag="VEA63_08465"
     CDS             <1..474
                     /locus_tag="VEA63_08465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD84074.1"
                     /translation="GLLYLLRWFWWRVNAWAEIAAMSASFVFALGFFFAQRHGLGWPA
                     HYTLLASVGGTTLVWLAVAYLAPATSAEVLKNFYTLVRPAGPGWRAVREQTGLAASTD
                     NLPAGILGAALGATAIWSALFATGSFLYGYTARGFALAAVALIGAWFVWRLLRKN"
     gene            complement(981..>1764)
                     /locus_tag="VEA63_08470"
     CDS             complement(981..>1764)
                     /locus_tag="VEA63_08470"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HYD84075.1"
                     /translation="YPMKLAPLFVRVVALLLLIGGSLCAQENAGTPPAAAKKNGYTVT
                     VDIKVDEKGQTGTVQLIETDDTSTGDVITKMALAMALKTELPVREKEGKPIKYTARVP
                     FFFPIEDDEGAAAAALPLPRPKQETGYMPAYPPALRAEGVTGGAILELFVDAEGKLTR
                     LTTLRASHPEFAAAATEAVQKWTFNPAQKDGQPVESRCRLAIVFETEENMAELKWRVA
                     PRPALGAFMVIRPDKPIDESMFEAAPAEGEAPAANTPAATKE"
BASE COUNT          312 a          597 c          486 g          369 t
ORIGIN      
        1 ggcttgctgt atttgctgcg ctggttctgg tggcgcgtga atgcctgggc tgaaatcgcc
       61 gcgatgtccg cctcgttcgt cttcgcgctc ggtttcttct tcgcccaacg tcacggcctc
      121 ggctggcccg cgcattacac gctgctcgcc tccgtcggcg gcaccacgct cgtctggctc
      181 gccgtcgcat atctcgcgcc cgcgacaagc gccgaagtgc tgaaaaattt ctacacgctc
      241 gtccgccccg ccggacccgg ctggcgcgcc gtgcgcgagc aaaccggctt ggccgcgtcc
      301 accgacaacc ttcccgcggg cattctcggc gcagcgttgg gcgcgaccgc catctggtcc
      361 gcgctcttcg ccaccggcag ttttctctac ggctacaccg cgcgaggctt cgcgctcgcc
      421 gccgtcgccc tcatcggcgc ttggttcgtc tggcgtctgc tgcggaagaa ctaaacatca
      481 cgcactggtc agccgaactc atgcggtagg tggagcccga cgtcccggtc gggctgtctg
      541 cgcgcctaat ccggacttcc gtagaagaga aatcacgaat cgacacgaat gaacacgaat
      601 tcagaggagc cgaaggacag ctttgaggct gatcgttacg tcgctcttcg gtgtcccgat
      661 aaggctccgc attcgaaggc gatttcactc gtgtcgattc gtgtccattc gtggttcaaa
      721 tgaattgctc cggctaattc atcggaacga ggaaaccaca gatgaacacc gatgaacaca
      781 gattcgaaag gccttcatct ccgtatccgt gttcatcggt gtccatccgt ggttgaaaag
      841 aatcggaatt attaaaccac gaataaacgc gaacagacac gcattcatat agccgtccga
      901 ccgccgacga tctctcttcc ctcgtgttca ttggggccaa ttcgtggttc caccccgaat
      961 tggccacgcg gccacggccg ctattccttc gtcgcagccg gcgtgttcgc cgccggcgct
     1021 tcgccctcgg cgggcgctgc ctcgaacatt gactcgtcaa tcggcttgtc cgggcgaatc
     1081 accatgaacg cccccaaggc cggccgcggc gccacgcgcc acttcagttc ggccatgttc
     1141 tcttccgtct cgaaaacgat cgccaggcga caacgcgact cgaccggctg cccgtctttt
     1201 tgcgccgggt tgaacgtcca tttttgcacg gcctcggtcg ccgccgcggc gaactccgga
     1261 tgcgatgcac gcagcgtcgt caaacgcgtc agcttgcctt ccgcatcgac gaacaattcc
     1321 aaaatcgcgc cgcccgtcac cccttcggcc cgcaacgccg gagggtaagc cggcatgtat
     1381 cccgtctcct gtttcggacg cggaagcggc aacgcggccg ccgcggcccc ttcgtcatcc
     1441 tcaatgggaa aaaagaacgg cacgcgcgcc gtgtatttga tcggctttcc ctctttttcg
     1501 cgcaccggca actccgtctt caacgccatc gcgagcgcca tcttggtgat gacatccccc
     1561 gttgacgtgt cgtcggtctc gatcaactga accgtccccg tctgaccctt ttcgtcgacc
     1621 ttgatgtcga cggtcacggt ataaccgttt ttcttggctg cggccggcgg cgttccagcg
     1681 ttttcctggg cgcacagcga accgccgata agcagcagca gcgcaaccac acggacaaag
     1741 agaggggcaa gcttcatagg gtag
//
LOCUS       DASPLT010000812         1134 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_296928, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000812 DASPLT010000000
VERSION     DASPLT010000812.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1134)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1134)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1134
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_296928"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..789
                     /locus_tag="VEA63_08475"
     CDS             <1..789
                     /locus_tag="VEA63_08475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD84076.1"
                     /translation="AVVLYDLAAADDCWRGIDTAFAHQRADGGFDAIVKPNGNSSREG
                     GAAVETTYFFLQELGRAILVIRQSPHEAHFRERLVALEPKLRRAADFILAGYDTIIPK
                     VGHTVNRVIIAAKAFGLCGLALDDPRMVEKSRQLIAHALTLRDAEGVFIEQGGRDSSY
                     NVVSILFGQVLALHLPLPEFEAALPAAVKWQLTRILPSGDVDVSGNTRTGVGLEADPF
                     SGKAKSVNYTEVAMALALYGTIHQDKEVLAAADRVFAFKQENGR"
     gene            846..>1134
                     /locus_tag="VEA63_08480"
     CDS             846..>1134
                     /locus_tag="VEA63_08480"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HYD84077.1"
                     /translation="MVGLWSVAVAAEPGRPNILFIYADDLGLGDVSCYNADAAWRTPH
                     LDRLAAEGMTFTDAHSASALCTPSRYALLTGRYAWRGRLKRAVVNGYDKALL"
BASE COUNT          185 a          360 c          370 g          219 t
ORIGIN      
        1 gcggtggtgt tgtacgatct cgccgcggcg gacgattgct ggcgcggcat cgacaccgct
       61 tttgcgcatc agcgggcgga tggcggtttc gatgcgatcg tgaagccaaa cggcaacagt
      121 tcccgcgaag gtggcgccgc cgtcgagacg acttactttt ttctgcagga gctggggcgt
      181 gccattctcg tcatccgcca gtcgccgcat gaggcgcatt ttcgcgaacg gctcgtcgcg
      241 ctcgaaccga agctgcgccg cgccgcggat ttcatcctgg caggttacga cacgatcatt
      301 ccgaaagtcg gccacacggt aaatcgcgtt atcatcgccg cgaaagcgtt cggtctctgc
      361 gggctggcgc tcgacgatcc gcggatggtg gagaaatccc gccagctcat cgcgcacgcg
      421 ttgacgttgc gcgacgcgga gggtgtgttc atcgagcagg gtggacgcga ttccagctac
      481 aatgttgtat ccattctgtt cggacaggtg ctcgcgcttc acctgccgct gcccgagttc
      541 gaggccgcac tgccggccgc ggtcaagtgg cagctgaccc gcattctgcc gtcgggcgac
      601 gtcgacgtga gcggcaacac ccgcaccggc gtgggcttgg aagccgatcc gttttcgggg
      661 aaggcgaaga gcgtgaacta cactgaagtc gcgatggcgc tcgccctcta tgggaccatt
      721 catcaggaca aggaagtcct cgcagccgcg gatcgcgttt tcgctttcaa gcaagagaac
      781 ggccgatgaa tctgcgcgca gtccggaccc acgccgttcg gttcggtctc gcggccggac
      841 tcgcgttggt tgggctgtgg tccgtcgccg tggcagcgga gcccgggcgg ccgaacattc
      901 ttttcattta cgccgatgac ctcgggctcg gcgacgtgtc ctgctacaac gccgacgccg
      961 cctggcggac gccgcatctc gaccgactcg ccgcggaagg aatgacgttc accgatgcgc
     1021 actcggcctc ggcgctttgc acgccgagcc gctatgcgct cctgacggga cgctacgcct
     1081 ggcgcggtcg gctcaagcgc gccgtcgtca acggctatga taaagccttg ctcg
//
LOCUS       DASPLT010000813         1104 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_296953, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000813 DASPLT010000000
VERSION     DASPLT010000813.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1104)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1104)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1104
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_296953"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..869)
                     /locus_tag="VEA63_08485"
     CDS             complement(<1..869)
                     /locus_tag="VEA63_08485"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007363951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HYD84078.1"
                     /translation="METPAASRRVVVTGLGVVTSIGHDVETFWSSLLAGKCGVQRVTL
                     FDPTDFACQIGAEVREWEAAQHMDPKEARRNDRYTHFGFVAAKQAIKDAGLDPAREDS
                     DRIGVMIGSGIGGMYTFETQLKVMAERGPRKVSPFTIPSLIGNMCSGLVAIDIGARGP
                     NFGLVSACATGTHAIGEAAHAIRRGDVDVMVAGGSEAAITPFAYASFCAMKAMSTHND
                     VPEKASRPFDRNRDGFIMGEGAGVLVLETLAHAQARGARIYCELIGYAATCDAFHITQ
                     PDPEGKGLSLAMSR"
BASE COUNT          215 a          388 c          303 g          198 t
ORIGIN      
        1 cgagacattg cgagcgagag gcctttcccc tcgggatcag gctgcgtgat atgaaacgca
       61 tcgcacgtcg ccgcgtagcc gatgagctcg caatagatcc gcgccccgcg cgcttgcgcg
      121 tgcgccagcg tttcgagcac cagcacgccg gcaccctcgc ccatgatgaa gccgtcgcga
      181 ttccggtcga aaggccggct ggccttctcc ggcacatcgt tgtgcgtgct catcgccttc
      241 atcgcacaga agctggcata cgcaaacggc gtgatcgccg cctcgctgcc gcccgccacc
      301 atcacgtcga catcgccccg gcggatcgcg tgcgccgcct cgccgatcgc atgcgtgccc
      361 gtcgcgcacg ccgacaccag gccgaaattc ggtccgcgcg cgccgatgtc gatcgcgacc
      421 aggcccgagc acatgttgcc gatcaacgat ggtatcgtaa aaggtgatac ttttctcggg
      481 ccgcgttccg ccatcacctt caactgcgtc tcgaacgtat acataccacc aatccccgag
      541 ccgatcatca ccccgatgcg atcgctgtcc tcacgcgcgg gatcgaggcc cgcgtccttg
      601 atggcctgct tcgccgcgac aaaaccgaag tgcgtgtagc ggtcattgcg ccgcgcttcc
      661 ttggggtcca tgtgctgcgc cgcttcccac tcgcgcacct ccgcgccgat ctggcaggca
      721 aagtccgtcg gatcgaacaa cgtcacgcgc tgcaccccgc acttgcccgc gagcaagctc
      781 gaccaaaacg tttccacatc gtgaccgatc gacgtgacca cacccaaacc ggtgacgacc
      841 acgcgtcgcg aagcagcagg agtttccatg agaaaagcgg agctggagtg ctaaaaacaa
      901 aaaggcgttc tgccagacat ttccgcgcgg gaaacaggca gaacgccgaa attcgaaaat
      961 cgcgagatta ggactggccg gccttcgctt tgatgtagtc ggtcacctgg ccaaccgtct
     1021 gcagcttctc ggcgtcggcc tccgggatct cacccttgat ctcgtctttg aactcctcct
     1081 caaacgccat gatcagctcg acgg
//
LOCUS       DASPLT010000814         2166 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_10494, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000814 DASPLT010000000
VERSION     DASPLT010000814.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 2166)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2166)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2166
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10494"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1046
                     /locus_tag="VEA63_08490"
     CDS             <1..1046
                     /locus_tag="VEA63_08490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007364235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HYD84079.1"
                     /translation="AFREALVDTIRNFFKRDYDAADVRQSIDAAIAVRVMEPVFESQT
                     KTKLGSATVGPNGPTLKDFVGKFMQQSLDVYLHKNKETAETIRTKIEENERERKELAG
                     IRNLARERAKKASLHNRKLRDCRLHLGDRNERAEESTLFIVEGDSAAGSLTATRDVQT
                     QAVFALKGKPLNTYGLSKKVIYENEEFHLLQSALNVEDGMDGLRYNKIVIATDADVDG
                     MHIRLLLISFFLQFFPDLIRNGHVFILETPLFRVRNKKRTIYCYSEQEKQAAVAELGA
                     SHEITRFKGLGEISAGEFKDFIGENIRLEPVSLMHLHDSEELLGFFMGKNTPERQDFI
                     IGNLKVEKDLVEV"
     gene            1157..>2166
                     /locus_tag="VEA63_08495"
     CDS             1157..>2166
                     /locus_tag="VEA63_08495"
                     /inference="COORDINATES: protein motif:HMM:NF015531.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HYD84080.1"
                     /translation="MLALAAGCATVPTEYREPAPLAAEERAALNGKVFDRVWELVNEK
                     YFDASFRGVDWAAMPERYRDEAVAATDDEGLYRVLNRMCAELKESHLVALTPRRVHEN
                     RVDHRAAIGVRWEMLEGKRVVVDVVPGGPAEEAGVQRGWLVVSRNGAPLVDGEVFITR
                     IGEPVTYGFLDEHETLRTFTFEPRLLNFERFEERVIAGGYVCLRFDEFGGASMTWLRR
                     MLREHRDAPGVILDLRNNHGGNTFALSAAVGNFFPHKVAEGRIVKRDGDTRESHSFGW
                     GSARYGGRVVVLVGPATGSAAEILTHVLQHHRRATAIGRRTAGAVIYSRLYRLPGGGR
                     LQV"
BASE COUNT          423 a          541 c          807 g          395 t
ORIGIN      
        1 cggcttttcg cgaagcgctg gtcgacacga tccgtaactt cttcaaacgc gactatgatg
       61 cggcggatgt gcggcaatcg atcgacgccg cgatcgcggt gcgggtgatg gagccggtgt
      121 tcgaatcgca gacaaaaacc aaactcggct cggccacggt cggcccgaac ggaccgacgc
      181 tgaaggattt cgtcggcaag ttcatgcagc agtcgctcga cgtctatctg cacaagaaca
      241 aggagacggc ggaaacgatc cggacgaaga tcgaggaaaa cgaacgcgag cggaaggagc
      301 tcgcgggcat tcgcaatctg gcgcgcgagc gcgcgaagaa agcgtcgctg cacaaccgca
      361 agctgcgcga ctgccggctg cacctcggcg atcgcaacga gcgcgcggag gagagcacat
      421 tgttcattgt ggaaggcgat tcggcggcgg gctcgctcac ggcgacgcgc gatgtgcaga
      481 cgcaggcggt gttcgcgttg aaggggaaac cgctgaacac ctacggactc tcgaagaaag
      541 tgatctacga gaacgaggag tttcacctgt tgcagtcggc gttgaacgtc gaggacggca
      601 tggacggact gcgctacaac aagatcgtga tcgcgaccga tgccgacgtg gacggcatgc
      661 acatccggct gctgctgatt tcgtttttcc tgcagttttt cccggacctg atccgcaacg
      721 gtcacgtgtt cattttggaa acgccgctgt ttcgcgtgcg aaacaagaag cgcacgatct
      781 actgctacag cgagcaggag aagcaggcgg cggtggcgga gctcggcgcg agccacgaga
      841 tcacgcggtt caaaggactc ggcgaaatct cggcgggaga gttcaaggat ttcatcggcg
      901 aaaatattcg gttggagccg gtgtcgttga tgcacctcca cgacagcgag gaactgctgg
      961 gcttcttcat gggcaagaac acgccggagc ggcaggattt catcatcgga aatctgaagg
     1021 tggaaaagga tttggtggag gtgtgaggcg gatggcggga ggcgagggtg gaggggcgcg
     1081 cggcggagcg ggcggctggg agcgcgcagg gcgcgcgcga aaaactcacg tttttcgctc
     1141 cgtgcttttg gcctgcatgc tcgcgttggc cgcgggttgc gcgacggtgc cgacggaata
     1201 tcgcgagccg gcgccgcttg ccgcggaaga gcgcgcggcg ctgaacggga aggtgttcga
     1261 tcgcgtgtgg gaactggtga acgagaaata tttcgacgcg agttttcgcg gcgtggactg
     1321 ggcggcgatg cccgagcgtt accgggatga agcggtcgcc gcaacggatg acgaaggact
     1381 ttatcgcgtg ctgaatcgga tgtgcgccga gttgaaagag tcgcatctgg tggcgttgac
     1441 gccgcggcgc gtgcacgaga atcgcgtcga tcatcgcgcg gcgatcggag tgcgttggga
     1501 aatgctcgag ggcaaacgcg tggtggtgga cgtggtgccg ggcgggccgg cggaggaggc
     1561 gggggtgcag cgcggctggc tggtggtgtc gcgcaacggc gcgccgctgg tcgatggcga
     1621 ggtgttcatc acgcgtatcg gagaaccggt tacatatgga tttctcgatg agcacgaaac
     1681 gttgcggacg tttacgtttg agccgcggct gttgaacttc gagcggttcg aggagcgggt
     1741 gatcgcagga ggttatgttt gcctgcgatt cgacgagttc ggcggcgcaa gcatgacatg
     1801 gttgaggcgg atgttgcggg agcatcgcga tgcgccgggc gtgatcctcg atctgcgaaa
     1861 caaccatggg ggaaacacgt ttgcgttgag cgcggcggtg ggaaatttct ttccgcataa
     1921 agtcgccgaa ggtcggatcg tgaaacgcga tggcgatacg cgagagtcgc acagttttgg
     1981 ctggggctcg gcgcgctacg gcggacgcgt ggtggtgctg gtcggaccgg caacggggag
     2041 cgcggcggag attttgacgc atgtgctgca gcatcaccgg cgtgcgacgg cgattgggcg
     2101 gcgcacggcg ggggcggtga tttattcgcg cctgtatcgg ttgccgggtg gcgggcgatt
     2161 gcaggt
//
LOCUS       DASPLT010000815         1173 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_10497, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000815 DASPLT010000000
VERSION     DASPLT010000815.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1173)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1173)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1173
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10497"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1173
                     /locus_tag="VEA63_08500"
     CDS             <1..>1173
                     /locus_tag="VEA63_08500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012377179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HYD84081.1"
                     /translation="AHTASTPWSQWTQFLLATPVVLWAAWPFFVRGARSFRSGHWNMF
                     TLIALGVGAAYLYSFAALFAPGLFPAAMRAHGTVDVYFEAAAVIVVLVLLGQVLELRA
                     RARTSSAIKSLLNLTPPTALRVTDSRDEEIPLEHVHVGDLLRVRPGGRIPVDGTLLEG
                     RSAVDESMLTGEPIPVEKTAGDKVTAGTVNGTGSFVFRAEKIGRDTMLARIVQLVADA
                     QRSRAPIQSLADRVAGWFVPAVAAIAVLTFAIWFWAGPEPRLAHAIINAVAVLIIACP
                     CALGLATPMSIMVGIGRGAHSGILVKQAEALERLEKVTTLVIDKTGTLTEGKPVLTDI
                     EPTPDLETKKPEPKTPNSGSHSEPRTPNPELRHPSAEPKTQNSDDLLQNLQPETQNP"
BASE COUNT          213 a          486 c          280 g          194 t
ORIGIN      
        1 gctcacaccg cgtccacccc ttggagccaa tggacgcaat tcctcctcgc gacaccggtc
       61 gtcctctggg ccgcctggcc cttcttcgtc cgcggcgcgc gctcgtttcg cagcggccat
      121 tggaacatgt ttacgctgat cgccctcggc gtcggcgctg cctacctcta cagcttcgcc
      181 gcgctgttcg cgcccggtct gttccccgcc gccatgcgcg cccacggcac cgtcgacgtc
      241 tactttgaag ccgccgccgt cattgtcgtc ctcgttctcc ttggccaagt cctcgagctc
      301 cgcgcccgtg cccgcacgtc ttccgccatc aaatcgctgc tcaacctcac gccgccgacc
      361 gccctccgcg tcactgattc ccgcgatgag gaaattcctc tcgaacacgt tcacgtgggc
      421 gacctgctcc gcgtccgacc cggcggacgc atccccgtcg acggcacgct cctcgagggc
      481 cgctccgccg tcgacgaatc gatgctcacc ggtgaaccca ttcccgttga gaaaaccgcc
      541 ggcgacaagg tcaccgcggg caccgtgaac ggcaccggct ccttcgtttt ccgtgcggag
      601 aaaatcggtc gcgacacgat gctcgcccgc atcgtccaac tcgtcgccga cgcccaacgc
      661 agccgcgcgc ccattcaaag cctcgccgac cgcgtcgccg gctggttcgt ccccgccgtc
      721 gccgccatcg ccgtgctcac gtttgcaatc tggttctggg ccggccccga accgcgcctc
      781 gctcacgcca tcatcaacgc cgtcgccgtc ctcatcatcg cctgcccctg cgccctcggc
      841 ctagcgaccc cgatgtcgat catggtcggc atcggccgcg gcgcccactc cggcatcctg
      901 gtgaaacaag ccgaagcgct cgaacgcctc gaaaaagtca ccaccctcgt catcgacaaa
      961 accggcaccc tcactgaggg caaacccgtc ctcaccgaca tcgaaccgac gcccgatctc
     1021 gaaaccaaaa aaccagaacc taaaacccca aactccggct cccactcgga acccagaact
     1081 ccgaaccccg aactccgcca ccccagcgca gaacccaaaa cccaaaactc cgacgattta
     1141 ctgcagaatc tccaacccga aactcaaaac ccg
//
LOCUS       DASPLT010000816         1221 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_33765, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000816 DASPLT010000000
VERSION     DASPLT010000816.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1221)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1221)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1221
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_33765"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            498..866
                     /locus_tag="VEA63_08505"
     CDS             498..866
                     /locus_tag="VEA63_08505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD84082.1"
                     /translation="MSKQQAEAAIRNANRAAKRGDLAEAERWSKTAERLGVTAEKLAA
                     VAHDSSSTDDEEWLRTVFMARLERLREVWDEGVLWEAEREIYERQCAEARARGADLPP
                     PLRMNPAPVERLDRIAAGEE"
     gene            972..>1221
                     /locus_tag="VEA63_08510"
     CDS             972..>1221
                     /locus_tag="VEA63_08510"
                     /inference="COORDINATES: protein motif:HMM:NF024846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HYD84083.1"
                     /translation="MNRKRVAIIGGGFSGASLAALLMQASPRPSVTLIERNARLGAGL
                     AYGANDGAHLLNVRASNMSAFATEPDHFARWLKARTGES"
BASE COUNT          273 a          388 c          386 g          174 t
ORIGIN      
        1 gcacacaaca aaagaaatct atccgccccc caaaaagctg agggataatc ctcctaccgt
       61 ttagcaggag agcagtcggc cgctgttcga aaccgatgcg agaacggtgt gatcgagcgt
      121 gatgcgtaga ggcttctcaa acttagagaa ggcgaggcgc gcagcgagca ataggcgtgg
      181 cgagcgccga tgacctgcaa gctgcgtgaa ccgtaacgcc aaagcgcaga agcccgtgtg
      241 aaaagcgcgg gaggatgcac aggaacgtcg tcgaacacga catccgcatg ccgccggccg
      301 agccgcctac ttgggtgaaa cacccctggc gacggcgccg atgggaaaac gatcacggga
      361 cgcgtggcat cgcgttcact tcgcgctcct tcaccggagc gcaactacca gaacgtgacg
      421 ccccgcgtcc cgctctccgt tttttccgat cgcaagatca acaagcatca cgcgcgccta
      481 aacgcgaagg agcagccatg agcaaacaac aagccgaagc cgctatccgc aacgccaatc
      541 gcgccgccaa gcgcggcgat ctcgccgaag ccgaacgctg gagcaaaacc gccgaacgcc
      601 tcggcgtcac cgccgaaaaa ctggccgcgg tggcgcatga ctcatcgagt acagacgatg
      661 aggaatggct gcgcaccgtg ttcatggcca ggctcgaacg actgcgcgaa gtctgggacg
      721 aaggcgtctt gtgggaagct gagcgcgaga tttacgaacg ccaatgcgcc gaagcgcgcg
      781 ctaggggcgc cgacctgccg ccgcctttgc gcatgaatcc cgcgccggtc gagcgattgg
      841 atcgtatcgc ggcaggcgaa gaatagggcg cccacgcaga gcgcgaagct atgcgctgac
      901 ggatggcaca agcggccgag tctgagcgat aatggctgga tgtgccttca acccaatccc
      961 ggacgtctcc gatgaatcgc aagcgcgttg cgatcatcgg cggcggtttc agtggcgcga
     1021 gcctcgccgc tctgctgatg caggcgagcc cgcgcccgtc ggtcacgctg atcgagcgca
     1081 acgcgcgcct gggcgccggg ctcgcctatg gcgcgaacga cggcgcccat ctgctcaacg
     1141 tgcgcgcctc gaacatgagc gcattcgcca ccgaaccgga tcatttcgca cgctggctga
     1201 aggcgcgcac gggcgagagc g
//
LOCUS       DASPLT010000817         1628 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_91127, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000817 DASPLT010000000
VERSION     DASPLT010000817.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1628)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1628)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1628
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_91127"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1140
                     /locus_tag="VEA63_08515"
     CDS             <1..1140
                     /locus_tag="VEA63_08515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007363291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HYD84084.1"
                     /translation="EQRRNETIVLTITGIGIAFGAIVAWWITRGVTRRITDVAARLAV
                     ESDKTGAAAVQVSQASQSMASGASEQAASLEETSSSLHEMASMTSRNSENAQNAKTLA
                     NQARQTADAGAIDMEQMKTAMAAIKGSSTEISKIIKTIDEIAFQTNILALNAAVEAAR
                     AGEAGLGFAVVADEVRNLAQRCAGAAKETADKISASTEKSEQGARISEKMASNLAAIV
                     DKTRQLDERIAEIAQSSHEQSEGIGQLNSAVASMDKITQENAALAEESSASAEELKSQ
                     AEQVRLAVAELMRMARGSEANDASDKKKSKKRAEEASTTATTVKTPILPRRNAPPART
                     LRQSNGSPVAAIADSSTHSGRNGHGEQANGGNEANGHEDADKFFA"
BASE COUNT          357 a          539 c          485 g          247 t
ORIGIN      
        1 gagcagcgcc gcaacgagac gatcgtgctg acgatcaccg gcatcggcat cgcattcggc
       61 gcgatcgtgg cctggtggat cacacgaggt gtcacccgcc gcatcaccga tgtcgctgca
      121 cgcctcgccg tcgaatccga caagaccggc gccgccgcgg ttcaggtttc ccaagcgagc
      181 caatcgatgg caagcggcgc gtcggaacag gcggcttccc tcgaggaaac cagttcctcg
      241 ctgcacgaga tggccagcat gacatcgcgc aattccgaga acgctcagaa cgccaaaacc
      301 ctcgcgaatc aagcgcggca aacagccgat gccggcgcca tcgacatgga gcagatgaag
      361 accgcgatgg ccgcgatcaa gggctccagc accgaaattt ccaaaatcat caagacgatc
      421 gacgagatcg cgtttcagac gaacatcctc gcgctgaacg ccgcggtcga agccgcccgc
      481 gccggcgaag ccggtctcgg tttcgcggtc gtcgccgacg aggtccgcaa tctcgcgcaa
      541 cgctgcgccg gcgccgcgaa ggaaaccgcc gacaagattt ccgcctcgac ggagaagagc
      601 gagcagggcg cgcgcatcag cgagaaaatg gcttccaacc tcgccgcaat tgtcgataaa
      661 acccgccagc tcgacgaacg catcgccgag atcgcgcagt cctcgcacga gcagagcgaa
      721 ggcatcggcc agctcaacag cgctgtcgcg agcatggaca agatcacgca ggaaaacgcc
      781 gcgctcgccg aggagtcctc cgcttcggcc gaggaactga aatcgcaagc cgagcaagtc
      841 cgcctcgccg tcgccgaatt gatgcgcatg gcccgcggct ccgaagcgaa cgatgcgagc
      901 gacaagaaga aatcgaaaaa gcgcgccgaa gaggcgtcga cgacggccac gaccgtgaag
      961 acaccgattc ttccgcgccg caatgcgccg ccggcccgca cgcttcgtca atcgaatggc
     1021 agcccggtgg ccgcgatcgc cgacagcagc acgcactccg gtcgcaacgg ccatggcgag
     1081 caagcgaatg gcggcaacga ggccaacggt cacgaagacg ccgacaagtt cttcgcctga
     1141 ggcattcgcg taaagtttct cactcaccaa gggcgtgcct cggcacgcct tttttttgtg
     1201 cgcgaacgag tggcagcgct gtaggtggtg cgcttgtcgc accgcgttgt gcgagcgcgc
     1261 acaacctgcg aacccacagg cagggcaccg cggcctgctg ctacacggaa gcgctcagcc
     1321 taagccggag ccatgcaatt gaaccacgca tggacaccga taaacacgga tccggcctcg
     1381 tggctgagga gcctaacgag acacgtttgg cttcaccaaa gcgtgagtct tttcaaatga
     1441 ccttttcgtg gtggaaagcg gttttcgatc cgtgtccatc cgtgttcatc ggtggtttcc
     1501 tgcggaagcg cctgccctaa gccgtcatcg catcttcctc gctccgcaca cgttcgtcga
     1561 ccagcaccgt cgtgacgaga tccgcgccga cgttcaccac ggtgcgcgtc atgtcgagga
     1621 ttcggtcc
//
LOCUS       DASPLT010000818         1101 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_214155, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000818 DASPLT010000000
VERSION     DASPLT010000818.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1101)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1101)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1101
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_214155"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(147..>1101)
                     /locus_tag="VEA63_08520"
     CDS             complement(147..>1101)
                     /locus_tag="VEA63_08520"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HYD84085.1"
                     /translation="RRFSRGKVKRMQPIISIKDVSKTYKGGVQALQPTSLDIMKGEIF
                     ALLGPNGAGKTTLINMTCGIVRLSSGAITADGDDIVRDYKTARKKIGLVPQELHTDAF
                     ERVIDTVTFSRGLFGFAPNPAYIEKVLRDLSLWDKRNAKIMELSGGMKRRVMIAKALA
                     HEPKILFLDEPSAGVDVELRRDMWEMVRGLRKNGVTIILTTHYIEEAEEMADRVGVIS
                     KGQLILVEEKSALMRKLGKKQLMLDLPAPIAQVPAGLNGYNVELANEGHQLIYSFDAQ
                     ANDTGIPAFLKRLTELGVDFKDLHTEQSSLEDIFVSLVHAK"
BASE COUNT          191 a          331 c          340 g          239 t
ORIGIN      
        1 gcgcggcttg tccgcccgca caatttcgcc aatgcgcgtc gccggcacag ccagatcaat
       61 agccaaccgg ttcgccgaaa gatcgagcgg cgccatgaac tcttccttga gtacttcgcc
      121 gggatgggtg cggatgcggg ggagactcat ttcgcgtgca ccagggaaac gaagatatct
      181 tcgagcgagg attgctcggt gtgcaaatcc ttgaagtcga cgccgagttc ggtcaggcgc
      241 ttcaggaagg cggggatgcc ggtatcgttg gcctgggcgt cgaaactgta gatcagctgg
      301 tggccttcgt tggccaattc gacgttgtag ccattcaagc ccgcgggcac ttgcgcgatc
      361 ggcgcaggca ggtcgagcat cagctgcttc ttgccgagct tgcgcatcag cgcgcttttc
      421 tcttccacca gaatgagttg gcctttgctg atgacgccca cccggtcggc catttcttcg
      481 gcttcttcga tgtaatgcgt cgtcagaatg atcgtgacgc cgttcttccg gagcccgcgc
      541 accatctccc acatgtcgcg ccggagctcg acatcgacgc cggcgctcgg ctcgtcgaga
      601 aagaggattt tgggttcgtg cgcgagcgcc ttggcgatca tcacccggcg cttcatgccg
      661 ccggacagct ccatgatctt ggcgttgcgc ttgtcccaca gcgaaagatc gcgcagcact
      721 ttttcgatgt aagccgggtt cggggcgaag ccgaacaggc cgcggctgaa ggtgaccgta
      781 tcgatcaccc gctcgaacgc gtccgtatgc agctcttgcg ggacgaggcc gatcttcttg
      841 cgcgcagttt tgtagtcgcg gacgatatcg tccccgtccg ccgtgatcgc gccggacgaa
      901 agccgtacga tcccgcaggt catgttgata agcgtcgtct tgccggcgcc gttgggacca
      961 agaagcgcga agatttcgcc cttcatgatg tcgagcgagg tcggctgcag cgcctggacg
     1021 ccgcccttat aggtcttgct cacatccttg atggagatga tcggctgcat gcgtttgact
     1081 tttccccgcg agaagcgccg c
//
LOCUS       DASPLT010000819         1787 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_235008, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000819 DASPLT010000000
VERSION     DASPLT010000819.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1787)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1787)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1787
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_235008"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1394)
                     /locus_tag="VEA63_08525"
     CDS             complement(<1..1394)
                     /locus_tag="VEA63_08525"
                     /inference="COORDINATES: protein motif:HMM:NF028237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase N-terminal 7TM
                     domain-containing protein"
                     /protein_id="HYD84086.1"
                     /translation="MPWQITAFLFSCVFELIMALFVRSRLRTLTSGPFCLALLLNASW
                     ALGYAIELGVPSLDDKLLVFQIRCSFLCFYAPAWFEMVHRMTRGRPLLRGWMIPAVLL
                     VPVVTLVLLWFPGPGQNPLLRHSFWIDATGGLPVLRDELGPWGFIYYFYNYAVWAVIF
                     LLLYPRKNHTAWERRGRQIFLAAAFIGWTADVLHLFHLSTPEGLNYAPALFPITSALI
                     AFALLRHRMLNLAPVARAALIERLEDRIIVFDGDNRVVDYNHAAAATLGLARSGVFGR
                     RADDVLGAWPELIAFFSTPRQSRRIEVTIGAVDFEASLVAVADEGHSSPRARVLVLSD
                     VTRRKETETQLRLAKEAAEAAGQAQSRFLATMSHEIRTPMNGVIGFIQLLKTTALEGK
                     QREYLDLIDHSTRSLLVIINDVLDYSKIAANQLEIEQIPCDLRLIVDHTHRLLQSFAA
                     EKKLAFTTGIAADVP"
BASE COUNT          344 a          465 c          690 g          288 t
ORIGIN      
        1 gggacgtcgg cggcgatgcc cgtggtgaac gcgagttttt tttcggcggc gaacgactgc
       61 agcagccgat gcgtgtgatc cacgatgaga cggaggtcgc aggggatctg ttcgatctcg
      121 agctggttgg cggcgatctt cgagtagtcg agcacgtcgt tgatgatgac gagcagcgaa
      181 cgggtgctgt gatcgatgag gtcgaggtat tcgcgctgct tgccctcgag cgcggtggtt
      241 ttgagcagtt ggatgaaacc gatgacgccg ttcatcggcg tgcggatttc gtggctcatc
      301 gtggcgagga aacgcgattg ggcctgaccg gcggcttcgg cggcttcctt ggcgaggcgg
      361 agctgggtct cggtttcctt gcgccgggtg acgtcgctga gaacgagcac acgcgcgcga
      421 ggactcgagt ggccttcgtc ggcgacggcg accagcgatg cttcgaaatc gacggcgccg
      481 atggtgactt cgatccggcg ggattggcgc ggcgtcgaga agaacgcgat gagttcgggc
      541 caggcgccga gcacgtcgtc ggcgcgccgc ccgaaaacgc cggatcgcgc gagcccgagg
      601 gtggcggcgg cggcgtgatt gtagtcgacg acgcggttgt cgccgtcgaa cacgatgatg
      661 cggtcctcga gccgttcgat gagggcggcg cgggcgaccg gggcgaggtt gagcatgcgg
      721 tggcgcagga gcgcgaaggc gatcagggcg ctggtgatcg gaaagagcgc gggggcgtag
      781 ttgagcccct ccggcgtgct gaggtgaaac agatgcagga cgtcggcggt ccagccgatg
      841 aaagccgcgg cgagaaaaat ctgccggccg cgtcgctccc acgcggtgtg gttttttcgt
      901 ggatacagta gaaggaagat aacggcccaa acggcgtagt tgtagaagta gtagatgaag
      961 ccccacgggc cgagctcgtc gcgcaggacg ggcagtccgc cggtcgcgtc gatccagaac
     1021 gagtgccgaa ggaggggatt ttgtccgggg ccgggaaacc agagcagcac gagcgtgacc
     1081 accggaacga gcaggacggc ggggatcatc cagccgcgca gcagtgggcg gccgcgagtc
     1141 atgcggtgca ccatctcaaa ccaagccgga gcgtagaagc agaggaacga gcagcgtatc
     1201 tggaaaacga gcagtttgtc gtcgagcgac ggcacgccga gctcgatggc gtagccgagg
     1261 gcccacgacg cgttgagcag cagcgcgaga cagaaaggcc cggaggtgag cgtccgcaac
     1321 cggctgcgga cgaacagcgc catgatcagc tcgaagacgc aggaaaaaag gaaagcggtg
     1381 atctgccagg gcacgcggcg tataaacgag gggaggcggg gctgaatgcc aagaagggag
     1441 atgaggcggt gaagctttcg tgcgcttatc gacgcgcgct gaactggagc aattcaattg
     1501 aaccacgacc aagcagaggc gcggacgcgg ggagcagcgg acggaaatcg gcggcggtaa
     1561 aggtaacagg gtcggacgtg cgccgagctt ggtttttggc tgtggtcgtt cgccggtcgg
     1621 ttggcatccg ccttgcgctt gttgaaagag accgcgctgg gcagcgtcgc gacatgacgc
     1681 gggtccagcg cgtgatgctt gtggacgatc accccgtcgt gcggtcgggg ctggcgctgc
     1741 tgctcgacca cttggaggga ttttcggtgt gcggcgaggc ggcgacg
//
LOCUS       DASPLT010000820         1046 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_10512, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000820 DASPLT010000000
VERSION     DASPLT010000820.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1046)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1046)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1046
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10512"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..180
                     /locus_tag="VEA63_08530"
     CDS             <1..180
                     /locus_tag="VEA63_08530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD84087.1"
                     /translation="NRNNENRNNENRNNENRNENRNEERRAENRNEQNRNEEQRNEER
                     RNEERRNEERRNERK"
     gene            254..>1046
                     /locus_tag="VEA63_08535"
     CDS             254..>1046
                     /locus_tag="VEA63_08535"
                     /inference="COORDINATES: protein motif:HMM:NF022113.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LpqB family beta-propeller domain-containing
                     protein"
                     /protein_id="HYD84088.1"
                     /translation="MPKAFSTEDICHYQTVSHLDFSPNAAFAVCTVQSTDLDDDKTES
                     AIWALPLDGGEPHQFSAGAGRDRAPRWSPDGSTIAFTSSRSGSPQIYLLSRDGGEARQ
                     LSHFASGASSAQWSPDGRYLLVICTLPVDPEKRGASGRDVDPCNERKDGPKLCWRLPY
                     KLDGVGFTLDAESHLFKLDANTGEHVRLTHGAVDVRSAAWSPDSRRVAFVQTREGRLA
                     NRTDIWVMDADGTHARQLTDSVAVVQSPSWSPDGNYLVFSGSEDEG"
BASE COUNT          231 a          334 c          308 g          173 t
ORIGIN      
        1 aaccgcaaca acgaaaaccg caacaacgaa aaccgcaaca acgaaaaccg taacgagaac
       61 cgcaacgaag aacgtcgcgc ggaaaatcgt aacgagcaga accgcaacga agagcagcgc
      121 aacgaagagc gccgcaatga agagcgccgt aacgaagaac gtcgcaacga acgcaaataa
      181 gtggctggcg ccggaggctc acgcttccgg cgccgttctt ccccggtttc gaatctgacg
      241 caaagtagtc gtcatgccca aagctttcag taccgaagac atctgccact accagacagt
      301 ttcccacctg gatttttccc ctaatgccgc ttttgccgtt tgcacggtgc agtccaccga
      361 tctcgacgac gacaagaccg agtcggctat atgggcactg ccactcgatg gcggcgaacc
      421 gcatcagttc tctgccggcg ccggtcgaga tcgcgcgccg cggtggtcgc ccgatggcag
      481 caccatcgcg ttcacatcat cacgcagcgg atcgccgcaa atttacctgc tgtcgcgcga
      541 tggcggagaa gcgcgccagc tgagtcactt cgcctcgggc gcatcgtcgg cgcaatggtc
      601 gccggacggc aggtatctgc tcgtcatctg cacgctgccc gtcgatcccg agaagcgggg
      661 cgcgtccggt cgcgacgtgg atccctgcaa cgaacgcaag gatggtccga agctgtgctg
      721 gcgcctgcct tacaagctcg atggcgtcgg cttcacgctc gatgccgaat cgcatctgtt
      781 caagctcgat gcaaacaccg gtgaacacgt gcgtcttacg catggcgctg tcgacgtgcg
      841 ctcggcggcc tggtcgccgg attcgcgtcg cgtggcgttc gtccagacac gcgaaggacg
      901 tctcgcaaac cgcaccgaca tatgggtgat ggatgccgac ggcacgcatg cacgccagct
      961 gaccgacagc gttgccgtgg tgcagtcgcc gtcatggtcg cccgatggca actacctcgt
     1021 gttcagcggc agcgaagacg aaggcg
//
LOCUS       DASPLT010000821         1009 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_235023, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000821 DASPLT010000000
VERSION     DASPLT010000821.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1009)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1009)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1009
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_235023"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..422
                     /locus_tag="VEA63_08540"
     CDS             <1..422
                     /locus_tag="VEA63_08540"
                     /inference="COORDINATES: protein motif:HMM:TIGR01509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HYD84089.1"
                     /translation="MLLRQQILPGVVSLLDAAGTHDLRIGLASNSEHAWVEPHLERLG
                     LRGRFEFLACREDAALPKPAADLYKLVLNRFGLRGHEAIAFEDSNTGCLAAKRANLHV
                     VAVPNASTAHHDFTPADLVVPSLAEVDLALLLTRWTQ"
     gene            complement(477..>1009)
                     /locus_tag="VEA63_08545"
     CDS             complement(477..>1009)
                     /locus_tag="VEA63_08545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD84090.1"
                     /translation="VRFECLYGEDWIATSPTEDALATAAQECGESAWQAFFEFVPADV
                     REFLERFSFTRMEALQVIARCPSLLSDLTETPALGAFVAAHANLRGTSGACWEEINVV
                     HERNGVFGLLEWLGLPASRPALGILQNIVTPDVPKRFLEPLRSMLWEPTALFALRRMP
                     AITDRELARTCHALAA"
BASE COUNT          187 a          418 c          246 g          158 t
ORIGIN      
        1 agatgctcct ccgccagcaa attctccccg gcgtggtttc actcctcgac gccgccggca
       61 cccacgatct ccgcatcggc ctcgcctcca actccgaaca cgcttgggtc gaaccacact
      121 tggaacgcct cggccttcgc ggccgcttcg aattcctcgc ctgccgcgaa gacgccgcgt
      181 tgcccaaacc cgccgccgat ctctacaaac ttgtgctcaa ccgtttcggc ctccgcggcc
      241 acgaagcgat cgcgttcgag gattccaata ccggctgcct tgccgcgaaa cgcgccaacc
      301 tccacgtcgt cgcggtcccc aacgcctcca ccgcgcacca cgatttcact cccgccgacc
      361 tcgtcgttcc ctcgctcgcc gaagtcgacc tcgccttgct gctcacccgc tggacgcagt
      421 agcggcaggc ctccgcgcct gccagcccgg taaaaagttt tcgcgcacgc cccacgtcac
      481 gccgccagcg cgtgacacgt ccgcgccagc tcgcgatccg tgatcgccgg catccgacgc
      541 agcgcaaaca gcgccgtcgg ctcccacaac atgctccgca acggttccag aaaacgcttc
      601 ggcacatccg gcgtcacgat gttctgcaaa atcccgagcg ccggccgcga cgccggcagc
      661 cccagccact cgagcagacc gaaaaccccg ttccgctcgt gcaccacgtt gatttcctcc
      721 cagcacgccc ccgacgttcc gcgcaagttt gcatgcgccg ccacaaacgc cccgagcgca
      781 ggcgtctccg tcaggtcgct caacagcgac gggcaacgcg caatcacctg taacgcctcc
      841 atccgcgtga acgaaaaccg ttccaaaaac tcgcgcacgt ccgccggcac gaactcgaaa
      901 aatgcctgcc acgccgactc gccgcattcc tgcgccgccg tcgccagggc gtcctccgtc
      961 ggcgaagtcg cgatccaatc ctcgccatac aggcactcga aacgcactt
//
LOCUS       DASPLT010000822         2578 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_70136, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000822 DASPLT010000000
VERSION     DASPLT010000822.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 2578)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2578)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2578
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_70136"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            269..2308
                     /gene="uvrB"
                     /locus_tag="VEA63_08550"
     CDS             269..2308
                     /gene="uvrB"
                     /locus_tag="VEA63_08550"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HYD84091.1"
                     /translation="MLFKLNADYQPTGDQPQAIEKLVGSIRAGNRYQTLLGVTGSGKT
                     FTMANVIAQCERPTLIISHNKTLAAQLYSEFKNFFPENAVEYFVSYYDYYQPEAYIAS
                     SDTYIEKDSSINEEIERMRIATSSSLVSRRDVIVIASVSCIYGLGSPQEFTDLRIQLR
                     RGETLGRNKFLERLVDNIYDRNDYELKRGTFRVRGDVVDVMPAYLEHGLRVEFFGDEI
                     EGISEFDPLTGMVMRVLDQFDLYPANQYVTSRGKLDPAIHAIKVELEERVAYFEGKGQ
                     YLEAQRIRMRTNYDLEMLQEMGFCNGIENYSRHLTGRQPGERPFCLIDFFPKDFLLMI
                     DESHATVPQIGGMSNGDRARKENLVNFGFRLPSALDNRPQTFAEFEEITGQTLYVSAT
                     PAQFELKQSAVIAEQLVRPTGLLDPEIAIHPTKGQVEHLIGEIKAATAAGERVLVTTL
                     TKRLSEDLTSYLREAKIRVEYLHSDIDAIERVEILRNLRLGNFDVLIGINLLREGLDL
                     PEVALVAILDADKEGFLRSETSLVQTAGRAARHEKGRVIFYADNITESIRRTVEIVKY
                     RREKQLAYNTAHGITPRSVKRAAQASLHVYDGSGERDEAAVAESIDDVAAVIAELEDD
                     MQEAAGRLEFERAAVLRDQINALKSGDYRKPGKAPAAYAPRKKNAAPKKAGRRRG"
     gene            complement(2199..2543)
                     /locus_tag="VEA63_08555"
     CDS             complement(2199..2543)
                     /locus_tag="VEA63_08555"
                     /inference="COORDINATES: protein motif:HMM:TIGR04256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GxxExxY protein"
                     /protein_id="HYD84092.1"
                     /translation="MLVEDCVLIEAKAVDHILPIHKAQLLSYMKLLNVPLGLLINFNV
                     TKTRRRRLAPHPPRRRPLTTPLPLLPPVKSGSLRPTPSAVPPSSARHSSSAAHTPPAP
                     CPASDNPPTSAR"
BASE COUNT          545 a          612 c          943 g          478 t
ORIGIN      
        1 acgcacgaca gtgagcaaag gagatggagt gtcggtcgaa gaccaaagat agcggtgtta
       61 cctccagacg tggcgggatg aggccagggg tggtgcctga tggcgtgcag atgaatgtcg
      121 catatgcgac atcggtgcgc acggcttagg gaggtgaggt tacgacgcgg cagcagggag
      181 gtcgtggccg gcccggaaag cgcgcggccg gcgggatggg tgagggaggt tgaaaacgcg
      241 cgggagttgg agtgagttgt cggcttcaat gctgttcaaa ctcaacgccg actaccaacc
      301 caccggagac caaccgcagg cgatcgagaa actcgtgggc tcgatccggg cggggaatcg
      361 ttatcagacg ctgttgggcg tcacggggtc gggcaaaacg ttcacgatgg cgaacgtgat
      421 cgcgcagtgc gagcggccga cgttgatcat ctcgcacaac aagacgctgg cggcgcagct
      481 ctattcggag ttcaagaatt tcttcccgga gaacgcggtc gaatattttg tcagctatta
      541 cgactactac cagcccgagg cgtatatcgc gtcgagcgac acttacatcg agaaagactc
      601 gtcgatcaac gaggagatcg agcggatgcg catcgcgacg agcagctcgc tggtgtcgcg
      661 gcgcgatgtg atcgtgatcg cgagcgtgtc gtgcatctac ggcttgggct cgccgcagga
      721 gttcaccgat ttgcggattc aactgcggcg aggcgagact ctcggccgga acaagttttt
      781 ggagcggctg gtggacaaca tttacgatcg taacgattac gagttgaagc gcgggacgtt
      841 ccgggtgcgc ggcgacgtgg tcgacgtgat gccggcgtat ctggagcacg gcttgcgcgt
      901 ggaattcttc ggggacgaaa tcgaggggat cagcgagttc gatccgttga cgggcatggt
      961 catgcgcgtg ctcgaccagt tcgatttata tccggcaaac caatacgtga cgtcgcgcgg
     1021 caagctcgat ccggcgattc acgcgatcaa ggtggagctg gaggagcggg tggcgtattt
     1081 cgagggcaag gggcagtatc tcgaggcgca acggatccgg atgcggacga actacgactt
     1141 ggagatgctg caggaaatgg gattctgcaa tggcatcgaa aattactcga ggcatctcac
     1201 ggggcggcag cccggcgagc ggccgttctg cttgatcgat tttttcccga aggatttctt
     1261 gctgatgatc gacgagagtc acgcgacggt gccgcagatc ggcggcatgt cgaacggcga
     1321 ccgggcgcga aaggagaatc tggtgaactt cggttttcgg ctgccgtcgg cgctcgataa
     1381 ccggccgcag acgtttgcgg agttcgaaga gatcacgggg cagacgttgt atgtttcggc
     1441 aacgcccgcg caattcgagt taaagcaatc ggcggtgatc gcggagcagc tcgtgcggcc
     1501 gacgggcttg ctcgatccgg agatcgcgat tcatccgacg aaagggcagg tcgagcattt
     1561 gatcggcgag atcaaggcgg cgaccgccgc gggcgagcgc gtgctggtga cgacgttgac
     1621 gaagcggtta tcggaggacc tcacgagcta tttgcgcgag gcgaagattc gcgtggagta
     1681 tttgcactcg gacatcgacg cgatcgagcg cgtggagatt ttgcggaatt tgcggctggg
     1741 aaatttcgat gtgctgatcg ggatcaattt gctgcgggaa ggactcgatt tgccggaggt
     1801 ggcgctcgtc gcaattctcg acgcagacaa ggagggtttt ctgcgcagcg agacgagttt
     1861 ggtgcagacg gcggggcgcg cggcgcggca cgaaaaggga cgcgtgattt tttacgccga
     1921 caacatcacg gagtcgattc gtcgcacggt tgagatcgtg aaatatcgga gggaaaaaca
     1981 gctcgcttac aacacggctc acgggatcac gccgcggagc gtgaagcgtg cggcgcaagc
     2041 gagcctgcat gtctacgacg gtagcgggga gcgcgatgaa gcggcggtgg cggaatcgat
     2101 cgacgacgtt gccgcggtga tcgcggagtt ggaggacgac atgcaggagg cggcggggcg
     2161 gctggagttt gagcgggcgg cggtgttgcg cgaccagatc aacgcgctga agtcggggga
     2221 ttatcggaag ccgggcaagg cgccggcggc gtatgcgccg cggaagaaga atgccgcgcc
     2281 gaagaaggcg ggacggcgga gggggtagga cgaagcgatc cggattttac aggagggagc
     2341 agagggagcg gagttgttag cggtcggcgt cggggaggat gaggcgcgag acgccgtcga
     2401 cgagttttgg tgacgttgaa attgatgaga aggccgagcg gcacgtttaa tagtttcata
     2461 tagctcaaga gttgagcctt gtggatgggg agaatgtgat cgacggcctt ggcttcgatc
     2521 aggacgcagt cctcgacgag caagtcgaag cggagaggct cgtctttgta gaagccct
//
LOCUS       DASPLT010000823         2389 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_194998, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000823 DASPLT010000000
VERSION     DASPLT010000823.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 2389)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2389)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2389
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_194998"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1363
                     /locus_tag="VEA63_08560"
     CDS             <1..1363
                     /locus_tag="VEA63_08560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD84093.1"
                     /translation="KVLRRSFATESEMTIDTIAWLAAVLWAVHPLRVEPVAWITASGY
                     PLATAFLLGSFGCYLTAYHDEHDTHGKKRRRWIAGAWALAALAYGSYPITATFGLWLI
                     AVDVGWLRIAPQPLWKITRADARVWWAKQLLFLAPALLALGVTVWSRLFSPGEFTHAP
                     GMANSPVADRGLMALASLGWFVVKFFWPTALTPNHQSLEIDVWSEPAIVALAAVTIVL
                     ALGLFLTRRKASSWIWIGAGFAGLSLPCLGLTEYPAWPVNRYSYLLDVVLVGTLAVAL
                     LSVVRRLRMLWPGVAPVAVGAAILTSMLAAYSSMWLLPIWRDSDRLFMHMENDPQTRR
                     SPALQAHLYLLWARHAARENLARGTTLRLNQAQRAYLSAIVGALQGGDFPRAVRLADR
                     LEADLGLTAVMRRERAGWLLKLGRTNEALSELAKVIEATPNDLRTRELLEEASGAESV
                     PTL"
     gene            1505..1702
                     /locus_tag="VEA63_08565"
     CDS             1505..1702
                     /locus_tag="VEA63_08565"
                     /inference="COORDINATES: protein motif:HMM:NF016167.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF433 domain-containing protein"
                     /protein_id="HYD84094.1"
                     /translation="MRLLNRITLKPGRRSGKPCIRDTRITVYDVFGYLAAGMTESEIL
                     QDFPDIGSGSRPCRWVLLRNW"
     gene            complement(1903..>2389)
                     /locus_tag="VEA63_08570"
     CDS             complement(1903..>2389)
                     /locus_tag="VEA63_08570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HYD84095.1"
                     /translation="GAALRTGFAAATGDAVIVQDADLEYDPQEYPRLMKPLIDGRADV
                     VFGSRFSGGDAHRVVYFWHMVGNKVLTLLSNMATNLNLTDMETCYKVFRRDVLQRITI
                     EENRFGFEPEITAKVAKLGVVIYEVGISYYGRTYAEGKKIGWRDGFRALWAIAKYNLF
                     R"
BASE COUNT          412 a          648 c          806 g          523 t
ORIGIN      
        1 caaggttcta cggcgttcgt ttgcgaccga gtcggagatg acaatcgaca cgatcgcgtg
       61 gctcgcggca gtgttgtggg ccgtccatcc gcttcgggtg gagccggtgg cttggatcac
      121 cgcgtcgggc tacccgctgg caacggcgtt tctgctcgga tcttttggct gttacctcac
      181 cgcgtatcac gatgagcatg acactcacgg caagaaacgg aggcgatgga ttgcaggtgc
      241 gtgggctttg gccgcgctgg cttatggaag ctatccgatt accgctactt tcggtctatg
      301 gttgatcgca gtggacgtgg gatggctgcg gatcgcaccg cagccacttt ggaagatcac
      361 tcgagccgat gcgcgcgtgt ggtgggcgaa gcaactcttg tttctggcgc ctgcgctgct
      421 cgccctcggg gtgacggtgt ggtcacggtt gttttcaccg ggcgagttta cgcatgcgcc
      481 gggcatggcg aattcgccgg tggccgatcg cgggttgatg gcgctcgctt cgctgggttg
      541 gtttgtcgtg aaatttttct ggccaacggc tctcacaccc aatcatcagt cactcgaaat
      601 cgatgtttgg agcgagccgg cgatcgtggc gttggcggca gtgacgatcg ttttggcgct
      661 cggattgttt ctgacacgaa ggaaggcgtc ttcctggatt tggattggag ctggctttgc
      721 cgggctgagt ctcccgtgtc ttggcctgac ggaatacccc gcgtggcccg tcaatcgcta
      781 cagctattta ctcgatgtcg tcctggttgg gacgctggcg gtcgctctgc tgtcggtggt
      841 tcgccgcttg cgcatgctct ggccgggcgt ggcgcctgtc gctgttggag ccgccatcct
      901 gacgtcgatg ttggctgctt actcgagcat gtggttgctc ccgatctggc gcgacagcga
      961 ccgccttttc atgcacatgg aaaacgatcc ccagacgaga cggagtccgg cattgcaggc
     1021 acacctctac ttgctgtggg cgcgacacgc ggcgagagag aatttggctc ggggcacgac
     1081 tttgcgactg aaccaggcac agcgcgctta tctctcggcc atcgtcggag ctttacaggg
     1141 aggtgatttt ccgcgcgccg tccggttggc ggatcggctc gaagccgatc tcgggttgac
     1201 agccgtgatg cgtcgggaac gtgctggctg gctgctgaaa ctaggacgca cgaacgaagc
     1261 gctgagcgag ttggcgaaag tgatcgaggc tactcctaac gatctgcgga cgcgcgagct
     1321 cttggaggag gcctcgggcg cggagagcgt tccaactttg tagcgaagca aagtcgagcc
     1381 gccgggcgca gccttacaag cgtgggaggc ggtgggcggc gaaattcgtg gaggcggggg
     1441 gagaggccgt agccgcactc ggtggaacgc cttgcaagca tacgacatct ttcatgctga
     1501 tcacatgcgc cttttgaatc gtatcacgtt gaagccggga cggcgaagcg ggaagccctg
     1561 cattcgcgac acacgcatta cggtgtatga tgttttcggt tatctcgccg ccggtatgac
     1621 agagtcggaa atattgcagg attttccgga tatcggttcc gggagtcgtc cgtgtcgttg
     1681 ggtgctcctc cgaaactggt aattttattg acgggaaatt gcccgacagg acacgtcgag
     1741 atctttgaga cgcgtgggtg gcgagttgga ggcgtttgaa cgcgatccgg tggcgtccat
     1801 attcgaagtc acgtgagcgt ttcgcgaggc agggcgcaga gcgcccgccc tacaaaggag
     1861 agaggcggcg gggtcgggag acgcccgccc tacacggggc ggtcagcgga agaggttgta
     1921 tttcgcaatc gcccagaggg cgcggaagcc gtcgcgccag ccgatctttt ttccctcggc
     1981 gtaggtgcgg ccgtagtagc tgatgccgac ttcgtaaatc accacgccga gcttcgcgac
     2041 tttcgcggtg atctcgggct cgaagccgaa acggttttcc tcgatggtga ttcgctgcag
     2101 cacgtcgcgc cggaacacct tatagcaggt ctccatgtcg gtgaggttca gattcgtcgc
     2161 catgttcgag agcaacgtga ggaccttgtt gccgaccatg tgccagaaat aaaccacgcg
     2221 gtgcgcgtcg ccgcctgaga agcgcgagcc gaacacgacg tcggcacgac cgtcgatgag
     2281 cggtttcatg aggcgcggat attcctgtgg atcgtattcg aggtccgcgt cctggacgat
     2341 gacggcgtcg ccggttgcgg cggcaaagcc ggtgcgcaac gccgcgcca
//
LOCUS       DASPLT010000824         2132 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_70159, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000824 DASPLT010000000
VERSION     DASPLT010000824.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 2132)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2132)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2132
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_70159"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..736)
                     /locus_tag="VEA63_08575"
     CDS             complement(<1..736)
                     /locus_tag="VEA63_08575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD84096.1"
                     /translation="MPAILERLKQHRAAVDEVHEGRRRKEQPRDEGKQIGLSLAKYPI
                     GFCRHIRDEVWDRALHDPEFLELVGPEVRVSRVFILLKGQFFQNAVQMGNLYVDVAND
                     TVWVHKPKLEWARLADVDYRNVETWAQFAEVARRYLKVELYPNLLFPLAFPIAPFFAI
                     RSNGRMDLLLAQDILFWKDVADGMPRARALLADEKFMNRRLPLDYEELLQKTAGCRAL
                     QGLPLKDHAVGIEAIRDGVVAQFAEIG"
     gene            complement(1106..1954)
                     /locus_tag="VEA63_08580"
     CDS             complement(1106..1954)
                     /locus_tag="VEA63_08580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD84097.1"
                     /translation="MVIASAATPELLWETPGFVGPESVVYDEARNELYVSNMGTHGNG
                     ATAGDGFISRVGSDGKILELKWVTGFDNPKGLALVKDRLFVGDDKDMVEIDVAAGKIV
                     ARHAPDDGGAGEFNDCTADPEGNVYVCSGRLGTVFRLHAGKFEPWVKLDRAKTGGING
                     LRAEKDRLLLGGWSVRDANGEEQLGHLSTIAYADRALGRLGDQPICHIDGIEPDGRGG
                     YTVTDWLTGDVLHVTKDGKPAPIMKLVRGTADHEYIEAKQQMLVPLMNDNVLRAYRWD
                     SAAENE"
BASE COUNT          404 a          763 c          532 g          433 t
ORIGIN      
        1 tccctatctc ggcaaactgc gcgaccaccc cgtctcgaat cgcttcgatg cccacagcat
       61 gatcctttaa gggaagccct tggagggcgc ggcacccggc ggtcttttgt agcagttctt
      121 cgtaatccag aggcaaccgc cgattcatga acttctcgtc cgccagcagt gctcgcgctc
      181 gcggcatccc gtccgcgacg tctttccaaa acaggatatc ctgagccagc agcaaatcca
      241 tgcgcccatt cgatcggatc gcgaaaaacg gcgcgatcgg gaacgccagc gggaaaagaa
      301 ggttcggata caattccacc ttcaaatatc gccgcgccac ttcggcgaac tgcgcccagg
      361 tttcgacgtt tcgataatcc acatccgcca aacgcgccca ctccaacttc ggcttgtgaa
      421 cccagaccgt gtcgttcgcc acgtccacat acagattccc catctgcacc gcattctgga
      481 aaaactgccc tttcagaaga atgaacactc gcgagacccg gacctccgga cccaccagtt
      541 cgagaaactc ggggtcatgg agcgctcgat cccagacttc gtcgcgaata tgccggcaga
      601 acccgatcgg atacttcgcg agcgacagtc cgatctgttt gccctcatcc cggggctgct
      661 cttttcgacg ccgcccttcg tgaacctcgt ccaccgctgc gcggtgttgc ttcaaacgct
      721 cgagaattgc cggcatcgcc ggaatcagtc gttccttggt tagccgctct tgtgcgaaaa
      781 cgaaccgcat gtcctgcgcg caagctcggg cgcgcaccgg gttcacggat gcttccccca
      841 gcttcgattt ccgcggcgtc atctcgcgtt tcgcgctccc gcgaccaccc aacggcagtg
      901 atccgccgct ccgaagccga tttttccccc ggaaatgggg tcgtgcccgt gccgcagatt
      961 ctccactttg gtcgttgctg ttacttccgg tgttcggcgc gctaaaaaat tcacgcggtg
     1021 gtgaatcgtc ttcagctcga agaacacaag ctcatttctc gacgctccct gctcatcgcc
     1081 gggcagggct cgaacacagc aacgattact cgttctccgc cgccgagtcc caacgatacg
     1141 cgcgcaggac attgtcgttc atcaacggca ccagcatctg ttgcttcgct tcgatatact
     1201 cgtgatccgc cgtcccgcgc acgagcttca tgatcggcgc cggcttgccg tccttcgtca
     1261 cgtgcaacac gtcgcccgtc aaccaatccg tcaccgtata accgccgcgc ccgtccggct
     1321 cgattccgtc aatgtggcaa atcggctgat cgcccagccg cccgagcgcg cgatccgcat
     1381 acgcgatcgt cgaaagatgc ccaagctgct cctcgccgtt cgcatcgcgc accgaccagc
     1441 cgccgagcaa caaccgatcc ttctcggcgc gcagtccgtt gattccaccg gtcttcgcgc
     1501 gatcgagctt cacccacggt tcgaactttc ccgcgtgcaa acgaaacacc gtgccgagcc
     1561 gcccgctgca cacgtaaaca tttccctccg gatccgccgt gcaatcgttg aactcacccg
     1621 cgccgccatc gtccggcgca tgccgcgcga cgatctttcc cgccgcgacg tcgatctcca
     1681 ccatgtcctt gtcgtcgccc acgaaaagcc ggtccttcac caacgcgagc cccttgggat
     1741 tgtcgaagcc cgtcacccat ttcagctcga gaattttccc gtcggacccg acgcggctga
     1801 tgaacccgtc gcccgccgtc gcgccgtttc catgcgtgcc catgttcgaa acgtagagtt
     1861 cgtttcgcgc ctcatcgtaa acgaccgact ccggcccgac aaaaccgggc gtttcccaaa
     1921 gcaactcggg cgtcgcggcg gacgcgatga ccacggcgaa tgaagcaacg acaagagcag
     1981 cgaataattt catgatgggg ttggggtgcg cgaacgctgc gccgcaccac cacacgtgac
     2041 aatccgatct tcgcgccgcg tgtgtcacac gcccctcctt cccgcgcccc atcgtcctcg
     2101 ttctctttct cgttctcgtt ctcgttctcg aa
//
LOCUS       DASPLT010000825         2385 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_235083, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000825 DASPLT010000000
VERSION     DASPLT010000825.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 2385)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2385)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2385
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_235083"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1245
                     /locus_tag="VEA63_08585"
     CDS             <1..1245
                     /locus_tag="VEA63_08585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDPGP type 1 family protein"
                     /protein_id="HYD84098.1"
                     /translation="VNLEGLEPAPYEPLPEHGGDAAAWAKAKTAGEEALRAGRVAAFT
                     VAGGQGTRLGYDGPKGTFPVTPLKQKTLFQVFAEKILAAGERYGRPLHWFIMTSHQNH
                     AATEAFFVEHRFFGMARERVHFFRQGRMPAVSFDGKILLESRHTIAMSPDGHGGSLRA
                     LDRSGALDVMAREGVDTLSYFQVDNPLVRCIDPAFIGWHLLRHSDMSSKMVPKAYPEE
                     KLGHFCAQNGQVVVIEYSDLPMEMQKQTDANGKLRYIAGSIAIHILDREFIRRMARGG
                     ESASLPFHRADKKIPTIDAQGAAVKAEKPNGVKFEMFVFDALPFAKNPVVIETRRADD
                     FSPVKNAEGVDSPKTCREDQLRQFARWLKANGAVVETDQTGLPQAAIEVSPRFGYDED
                     SFADRWKKLSPKPAVRDGLYLE"
     gene            1332..>2385
                     /locus_tag="VEA63_08590"
     CDS             1332..>2385
                     /locus_tag="VEA63_08590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009508182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HYD84099.1"
                     /translation="MSTLDRIQAAVQAGKLLPSAAENLSTFLSANLPDWAQTSIEELV
                     TKEAWSELNDRFYRYLEFGTGGMRGRTIGMVSAAAETGTLNAAGSPEHAAIGSNMLND
                     FTLIRAVVGLHRYTAKYLASRGVAETPRLVIAHDVRHFSRRFCELAAATWVRLGGEAF
                     IFDGPRSTPQLSFSVRWLKAHAGVVITASHNPPHDNGFKAYFDDGAQVVPPHDKGIVT
                     EVNATPLGELSGFVSADLRGVTTLGRDADDAYLAVAAKAAVDPQVFRKTKLSVVFTNI
                     HGTGAVQSLPLLIHAGCHVEAVPEQLEFDARFPTVKSPNPENSAALALAVALAEKTNA
                     DVVLATDPDADRMGCAV"
BASE COUNT          441 a          664 c          851 g          429 t
ORIGIN      
        1 gtgaacctgg agggcctcga gccggcgcct tacgagccgt tgccggaaca tggtggcgat
       61 gcggcggcct gggcgaaggc gaagaccgca ggcgaggaag cgctgcgcgc gggccgcgtg
      121 gcggcgttca ccgtcgcggg cgggcagggc acgcggcttg gttacgacgg gccgaaaggc
      181 acgtttccgg tgacgccgtt gaaacagaag acgctgttcc aggtgttcgc ggaaaaaatt
      241 ctcgccgcgg gcgaacgcta tgggcgtccg ttgcactggt tcatcatgac gagccaccag
      301 aatcacgcgg cgacggaggc gtttttcgtg gagcatcgtt tcttcggaat ggcgcgcgag
      361 cgcgtgcatt ttttccggca ggggcggatg ccggcggtga gtttcgacgg gaagattttg
      421 ttggagtcgc ggcacacgat cgcgatgtca ccggatggac acggcggatc gttgcgcgcg
      481 ctcgatcgca gcggcgcgct cgacgtgatg gcacgcgagg gcgtcgacac gctcagctat
      541 ttccaagtgg ataatccgct cgtgcgctgc atcgatccgg ccttcatcgg ctggcatctg
      601 ctgcggcatt ccgacatgtc gagcaagatg gtgccgaaag catatcccga ggagaaactc
      661 ggccatttct gcgcgcaaaa cgggcaggtt gtcgtgatag aatactcgga tttgccgatg
      721 gagatgcaga agcagacgga cgcgaacggg aagctgcgct acatcgcggg cagcatcgcg
      781 atccatattc tcgaccgcga attcatccgg cggatggcgc gaggcggcga gagcgcatcg
      841 ctgccgtttc atcgcgcgga taaaaaaatc ccgacgatcg acgcgcaggg ggcggccgtg
      901 aaagcggaga agccgaatgg cgtgaagttc gagatgttcg tgttcgacgc gctgccgttt
      961 gcgaagaatc ccgtggtgat cgaaacgcgc cgcgccgacg atttctcgcc ggtgaaaaac
     1021 gccgagggcg tcgactcgcc caaaacctgc cgcgaggatc aacttcggca gttcgcgcgt
     1081 tggttgaagg cgaacggcgc ggtcgtcgaa acggaccaga cgggtttgcc gcaagcggcg
     1141 atcgaggtgt cgccgcgttt cggctatgac gaagattcgt tcgccgaccg gtggaaaaaa
     1201 ctgtcgccga agccggcggt tcgtgacggt ctctatttgg aataaccggc ggctgggttg
     1261 ggtttcgggc gaggcgtcgc ggacgcagcg ttgcgacccg cccggaggtc gggccccagc
     1321 caaacttttt catgagcaca ctcgatcgca ttcaagccgc cgtccaagcg ggcaagctcc
     1381 tgcccagcgc tgccgaaaat ctctcgacgt ttttgtcggc caatctgcct gactgggcgc
     1441 agacgagcat cgaggaactc gtcacgaagg aagcatggag cgagttgaac gaccgctttt
     1501 atcgttatct cgaattcggc acgggaggaa tgcgcggccg cacgatcggc atggtgtcgg
     1561 cggcggcgga gacggggacg ttgaacgcgg ccggttcgcc ggagcacgcg gcgatcggca
     1621 gcaacatgct gaacgacttc acgctgattc gcgccgtggt tggactccat cgttatacgg
     1681 cgaagtatct cgcatcgcgc ggggtcgccg agacgccgcg gctcgtgatc gcgcacgacg
     1741 tgcgacattt ctcgcggcga ttttgcgagc tggcagcagc gacgtgggtg cggttgggcg
     1801 gagaggcgtt tattttcgac gggccgcgtt cgacgccgca gctgagtttc agcgtgcgct
     1861 ggttgaaagc gcatgccggc gtggtgatca cggcgagcca caatccaccg cacgataatg
     1921 gcttcaaagc ctacttcgac gacggtgcgc aggtggtgcc gccgcacgac aaaggcatcg
     1981 tgaccgaggt gaacgcgacg ccgctcgggg agttgtcggg attcgtgtcg gcggatctga
     2041 ggggcgtgac gacgctcggg cgcgacgcgg acgatgccta tttagcggtc gcggcgaagg
     2101 cggcggtgga tccgcaggtg tttcggaaga cgaagttgag cgtggtgttc accaacatcc
     2161 atggcacggg cgcggtgcag tcgttgccgt tgttgattca cgcgggctgt catgtcgaag
     2221 cggtgccgga gcaactggag ttcgacgcgc gcttcccgac cgtgaaatcg ccgaacccgg
     2281 agaattccgc ggcgctcgcg ctggccgtgg cgctggcgga gaagacgaac gcggacgtgg
     2341 tgctcgcgac ggacccggat gcggatcgca tgggctgtgc ggtga
//
LOCUS       DASPLT010000826         1112 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_49682, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000826 DASPLT010000000
VERSION     DASPLT010000826.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1112)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1112)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1112
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_49682"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            32..805
                     /gene="lptB"
                     /locus_tag="VEA63_08595"
     CDS             32..805
                     /gene="lptB"
                     /locus_tag="VEA63_08595"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007359422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HYD84100.1"
                     /translation="MTPNPAIADTAPTTPAPTSEIRTEGLAKVFGDRRVVDGVNMRFS
                     AGEVVGLLGPNGAGKTTTFYMIVGLISATDGRVCLDGTDLTRQRMHQRARHGIGYLPQ
                     EPSVFRKLTVEQNILAILESTGVRRRDRAARVQAHLEELHLTHVAKQKAYTLSGGERR
                     RLEIARALVTRPKFLLMDEPFAAIDPISVAEVQKIILQLKSRGIGIVITDHNVRETLR
                     VVDRAYLIHQGKVLAEGTGEFLIHDEQARKFYLGESFNL"
     gene            886..>1112
                     /locus_tag="VEA63_08600"
     CDS             886..>1112
                     /locus_tag="VEA63_08600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012377010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr(Ser) kinase/phosphatase"
                     /protein_id="HYD84101.1"
                     /translation="MQKAIHGITVAHFLETYRVKLKMELVTGEAGLHRLIREGSINRP
                     SLALTGFFKYFANKRIQVLGAAEMTFLKTVPP"
BASE COUNT          218 a          429 c          279 g          186 t
ORIGIN      
        1 tgcaccacga catttttcca acgttgtccc gatgaccccc aatcccgcca tcgctgacac
       61 cgcgcccacg acgcctgcgc ccacttccga aatccgcacc gagggtctcg cgaaggtctt
      121 cggcgatcgc cgtgtcgtcg acggcgtgaa catgcgtttc agcgccggcg aagtcgtcgg
      181 cctgctcggc cccaacggcg ccggcaaaac caccacgttc tacatgatcg tcggcctcat
      241 ctcggcgacc gacggccgcg tctgcctcga cggcaccgac ctcacgcgcc agcgcatgca
      301 ccagcgcgcc cgccacggca tcggttatct gccgcaggaa ccgtcggtgt tccgcaagct
      361 caccgtcgag caaaacatcc tcgcgatcct cgaatccacc ggcgtccgcc gccgcgatcg
      421 cgccgcccgc gtgcaagccc acctcgagga acttcacctc acgcacgtcg cgaaacaaaa
      481 agcctacacg ctctccggcg gcgagcgccg ccgcctcgag atcgcgcgcg ccctcgtcac
      541 gcgtccgaaa ttcctgctga tggacgagcc cttcgccgcg atcgatccga tctccgtcgc
      601 cgaggtgcag aagatcatcc tccagctcaa gtcccgcggc atcggcatcg tcatcaccga
      661 tcacaacgtc cgcgaaaccc tccgcgtcgt cgaccgcgcc tacctcattc accaaggcaa
      721 ggtgctcgcc gaaggcaccg gcgaattcct catccacgac gaacaagccc gcaaattcta
      781 cctcggcgaa agcttcaacc tgtaaccggg gtggcgcgtg gcggaggcga gcgcgccaaa
      841 ttctgctcta ctcgctactc actacccgct actcgctact gcagaatgca aaaagccatc
      901 cacggcatca ccgtcgccca tttcctcgag acctatcgcg tgaagctcaa gatggagctc
      961 gtcaccggcg aggccggcct ccaccgcctc atccgcgaag gcagcatcaa ccgcccctcg
     1021 ctcgcgctca ccggcttttt caaatatttc gcgaacaaac gtatccaggt tttgggtgcg
     1081 gctgagatga cgtttctcaa aaccgtcccc cc
//
LOCUS       DASPLT010000827         1550 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_49697, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000827 DASPLT010000000
VERSION     DASPLT010000827.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1550)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1550)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1550
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_49697"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..515
                     /locus_tag="VEA63_08605"
     CDS             <1..515
                     /locus_tag="VEA63_08605"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HYD84102.1"
                     /translation="ASEGRGRGAKFTIELPLLRTDPHVASEPEPLPAVPLTENAATRP
                     VVRGRVLLVEDHAPTRSTLQQLLIRRNYEVVAAETMAEARALMETERVNFVISDIGLP
                     DGNAYDLMEELREKHGLTGIALTGYGMESDIARSHAAGFVIHLTKPLRVQLLDEALVK
                     LAAHLSSTQR"
     gene            689..1480
                     /gene="panB"
                     /locus_tag="VEA63_08610"
     CDS             689..1480
                     /gene="panB"
                     /locus_tag="VEA63_08610"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HYD84103.1"
                     /translation="MPTIREFAAAKTKRRKIAVVTAYDAWSARLLADSGVDALLVGDS
                     AMMVMHGERDTLGATVELMALHTRAVARGAPDKLIVADLPFLAARKRTAYALDAAAQL
                     MRAGAHAVKIEGVRGHAEVIRHLVESGIPVMGHLGLQPQSVHAVGGYFVQGRETGEAA
                     QLREDCAAVEAAGAFALVLECVPAKLARAVTADLRIPTIGIGAGAGCDGQVLVLHDLL
                     GFNPEFKPRFVRRFADGAAVVKKGVAAYVAAVRSKKFPAPKESFT"
BASE COUNT          269 a          451 c          562 g          268 t
ORIGIN      
        1 cggcgagcga aggccgtggg cgcggcgcga aattcacgat cgagctgcca ttgttgcgca
       61 cggacccgca cgtcgcgagc gagcccgagc cattgcccgc cgtgccgctc accgagaatg
      121 ccgccacacg tccggtcgtg cgcggtcgcg tgctgctggt cgaggatcac gcgccgacgc
      181 gatcaacgct ccagcaactg ctcatccggc gtaattacga ggtggttgcc gccgagacga
      241 tggccgaggc gcgggcgttg atggaaaccg aacgggtgaa ctttgtgatc tccgacatcg
      301 gcctgcccga cggcaacgcc tacgatttga tggaggagtt gcgcgaaaag cacggactca
      361 ccgggatcgc gctcaccggt tacgggatgg aaagcgacat cgcgcgcagc cacgcggcgg
      421 gttttgtgat tcaccttacg aaaccgctga gggtgcaatt gctggacgaa gcgttggtga
      481 aactggcggc gcatctgtct tcgacacaaa ggtaacaagg gccgcaaacg atattgcagc
      541 gagctggcgg atggcgccgg gctgcggcaa catcggctca ttgcgagtga tggcgcttcg
      601 gagaaagtcc ccgcgcacgc ccgttgatcg ctgacgcagc gcgcccttgt gcggcgcgga
      661 taagtgtgtt gggtatcgcg ttccgcgcat gccaacgatc cgagaattcg ccgcggccaa
      721 gaccaagcgc cgcaaaattg ccgtcgtaac cgcatacgac gcctggagcg cgcggctgct
      781 cgcggattcg ggcgtcgatg cgttgctggt cggcgacagc gccatgatgg tgatgcacgg
      841 cgaacgcgat acgctgggcg caacggttga gttgatggcg ttgcacacgc gagcggtcgc
      901 gcgaggcgct ccggacaagc tgatcgtcgc agacctgcca tttctcgccg cgcggaaacg
      961 caccgcttat gcgctcgatg ccgccgcgca gttgatgcgg gcgggcgcgc atgcggtgaa
     1021 aatcgagggt gtgcgcgggc acgcggaagt gatccggcac ctggtcgaat cgggcattcc
     1081 cgtgatgggc catctcggtt tgcagccgca gtcggtgcac gcggtcggcg gttattttgt
     1141 gcaaggccgc gaaaccggcg aggcggcgca gttgcgcgaa gattgcgcgg cggtcgaagc
     1201 ggcgggcgcg tttgcgttgg tgctcgagtg cgtgccggcg aaactcgcgc gcgcggtgac
     1261 ggccgacttg cgcatcccga cgatcggcat cggtgccggt gcgggttgcg atggacaggt
     1321 gctcgtgttg cacgacctgt tgggtttcaa cccggagttc aagccgcgct tcgtgcggcg
     1381 ttttgcggat ggggccgcgg tcgtgaagaa gggcgtcgcc gcctatgtcg ccgcggtgcg
     1441 cagcaaaaag ttcccggcgc cgaaggagag ttttacgtga agaggtggac tgaaagcaga
     1501 tcgggctcac gattgacggc tgcgtggacg cgccgcggcc gtggcgggct
//
LOCUS       DASPLT010000828         2464 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_33868, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000828 DASPLT010000000
VERSION     DASPLT010000828.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 2464)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2464)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2464
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_33868"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1006)
                     /locus_tag="VEA63_08615"
     CDS             complement(<1..1006)
                     /locus_tag="VEA63_08615"
                     /inference="COORDINATES: protein motif:HMM:NF012576.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HYD84104.1"
                     /translation="MKATLRSLAEKCGLSPSTASRALSGHPNVRPEVRAQVQAEAKRL
                     GYTRNHLVGAVMSHVRAARTDRFLGNLAIVHVAAPEQPRPRPMQQVMIDAARQRAREI
                     GFRVDVLSLDRTTTSATALGRTLRARGVQGVIFLYSKLTDAAQGFPWEHFASVEIDYG
                     SPTLFVHTVAIDHHLTLHNALVRLRAMGYRNIGLFIERYKDERLLNKWTATFRSFQEL
                     QGGIGNAPLLLEDTMTADAFLAWHQKHKLDLVIGHVDEAVAWLRSSGIAIPKQTSFFN
                     LNWNERNRPCAGLDLRPELQGTAAVDSVVAQIHRNECGLPVDPHTVMVSGRWVDGATL
                     R"
     gene            1157..2413
                     /locus_tag="VEA63_08620"
     CDS             1157..2413
                     /locus_tag="VEA63_08620"
                     /inference="COORDINATES: protein motif:HMM:NF019113.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HYD84105.1"
                     /translation="MLGTVVAAAATTPEKVDLSGRTEESIREVVRRVARHHLRSLGDG
                     EYPAEATIDVLRTARPPTGVQWTYPWGVTLYGMLRSTDATGDVEVREFVREHNRIIAR
                     YFAWLEGVREKVGGTPEWDAFIRDDARTPLRRLLLLGNLDFCGAMGVEMLEDQLRHPE
                     AKTPEQHAVVARVADWIGSRQFRLPDGTVWRPDRTDVKEVMPRGTLWIDDLYMACPFL
                     VRWGQSTGDDRHLRDAARNVINMAARMQDRDGVWFHAYFENAAKASPWKWGRANGWAM
                     VATVEILSAMPDEHPDRPALLEILRKHIDGLKPLQAESGLWRQVLDRSELWEETSCTA
                     MFSYCIARAVNRGWIDRSYLELARRGFAGVAGNVTPEGVVKGTCQGTNIGLDLDYYVN
                     RLRPDDDLHGPGVVLLAGTEILAAGR"
BASE COUNT          417 a          675 c          905 g          467 t
ORIGIN      
        1 gtcgcagggt ggcgccatcg acccagcggc cgctgaccat cacggtgtgc ggatcgacgg
       61 gcaggccgca ctcgttgcgg tggatttgtg cgaccacgga atcgacggcg gccgtgcctt
      121 gtagttccgg gcggagatcg aggccggcgc acgggcggtt gcgttcgttc cagttgagat
      181 tgaaaaagct cgtctgtttc gggatggcga tgccgctgct acgcaaccag gcgacggctt
      241 catcgacgtg tccgatcacg aggtcgagct tgtgtttctg gtgccacgca agaaaggcgt
      301 cggcggtcat cgtgtcctcg agcagcagcg gtgcgttgcc gatgccgcct tggagctctt
      361 gaaaggagcg gaacgtagcg gtccatttgt ttagcagtcg ctcgtcttta taacgttcga
      421 tgaagagccc gatgttgcgg taacccatcg cgcggagacg cacgagagcg ttgtggagcg
      481 tgagatgatg gtcgatcgcg acggtgtgca cgaaaagcgt gggcgagccg taatcgattt
      541 cgacggaggc gaagtgctcc cacggaaaac cctgggcggc gtcggtgagt tttgaataca
      601 ggaagatcac gccttggacg ccgcgggcgc gcagggtgcg gccgagcgcg gtggcgctgg
      661 tcgtggtccg gtcgaggctg agcacgtcga cgcggaagcc gatttcccgc gcgcgctggc
      721 gcgcggcgtc gatcatcacc tgctgcatcg gccgcgggcg cggctgctcg ggggcggcga
      781 cgtgcacgat cgcgagattg ccgaggaagc ggtcggtgcg cgcggcccgg acgtgcgaca
      841 tgacggcgcc gacgagatgg ttgcgggtgt agccgagtcg ctttgcctcg gcctgcacct
      901 gcgcccgcac ctcggggcgc acgttgggat gacccgaaag cgcgcgcgag gcggtcgagg
      961 gggagagacc gcatttttcg gcgagggagc ggagggtcgc tttcattgca acgcatgcaa
     1021 aagtgccggg ctgaccggcg cagccaagtc gaattttgtc gctgcgttgc gcggttcgat
     1081 gtgagccgcg cgacgattct tcacccctat gctgaattcc gtcgctccgt tttttcgtgc
     1141 cgcgtggtgc ctggccatgc tgggaaccgt ggtggccgcc gccgcgacga cgcctgaaaa
     1201 ggtcgatctt tccggacgca cggaggaatc cattcgcgaa gttgtccgcc gcgtggcgcg
     1261 gcatcatctt aggtcgttgg gggacggcga gtatccggcg gaggcgacca tcgatgtcct
     1321 gcgcacggct cgcccgccga cgggggttca gtggacgtat ccctggggcg tgacgcttta
     1381 cggcatgttg cgttcgacgg atgccaccgg cgacgtggag gtgcgcgaat ttgtgcgtga
     1441 gcacaaccgg atcatcgcgc gttattttgc gtggctcgaa ggcgtccggg aaaaagtggg
     1501 aggaacgccg gaatgggatg cgttcatccg cgatgacgcg agaacgccgc tgcggcggct
     1561 gctgctgttg ggcaatctgg atttttgcgg agccatgggc gtggagatgc tcgaggacca
     1621 gctgcggcat cccgaggcga aaacgccgga gcagcacgcg gtggttgcac gggtggctga
     1681 ttggattggg tcgcggcagt ttagacttcc ggacggcacg gtgtggcggc cggatcggac
     1741 ggacgtgaag gaagtgatgc cgcgaggcac gttgtggatc gacgatcttt atatggcttg
     1801 tccgtttctt gtgcgctggg gccagtcgac cggtgacgac cgccatctgc gggatgcggc
     1861 gcgaaacgtg atcaacatgg cggcgcgcat gcaggatcgg gacggcgtat ggtttcacgc
     1921 gtatttcgaa aacgccgcca aagcctctcc ctggaaatgg ggccgggcga atggctgggc
     1981 gatggtcgcc acggtggaaa ttctttcggc gatgccggac gaacatccgg accgaccggc
     2041 gttgttggag attttacgaa agcacatcga cggattgaaa ccgttgcagg cggagagcgg
     2101 actgtggcgg caggtgctcg accggtcgga actctgggaa gagacctcgt gcacggcgat
     2161 gttcagctac tgcatcgcgc gcgcggtcaa ccggggatgg atcgatcgct cgtatcttga
     2221 actcgcgcgc cgcggtttcg ccggcgtggc cggcaacgtg acgccggaag gcgtggtgaa
     2281 aggaacgtgc cagggcacga acatcgggct cgatctggac tactatgtga atcgcctgcg
     2341 gccggacgac gacctgcacg gccccggagt cgtgctgcta gcgggcacgg aaatcctggc
     2401 ggcaggacga tgaactgtgc gtttcttttg tcgccaagcg tgcggcgttc accggagctc
     2461 tcct
//
LOCUS       DASPLT010000829         2321 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_133488, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000829 DASPLT010000000
VERSION     DASPLT010000829.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 2321)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2321)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2321
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_133488"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1845)
                     /locus_tag="VEA63_08625"
     CDS             complement(<1..1845)
                     /locus_tag="VEA63_08625"
                     /inference="COORDINATES: protein motif:HMM:NF012686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD84106.1"
                     /translation="MSALRPADLVIVGVYLAATLLFGLWMRKRATQSLEHYYLGGRRM
                     PWPLLGISGMANWFDLTGTMIITSFLFMLGPRGLFIEFRGGAVLVLAFMLLWTGKWYR
                     RSKCMTGPEWYVFRFGAGRAAEALRVISAVFGIVFTIGMLAYLVRGTSLFLGVFLPLS
                     PMAATVIVIAFSTLYTMIAGFYGVIVNDLVQGIIIVVTCLIVAALAFNLVPDAATLAV
                     LAERVTGNAEWMSAMPAVHTTMPAGYEAYESLLLFAGFYLVKSIFDGAGSGDDARYFA
                     ARSDRDCGLLSLMQGVTVAFRWPMMMGFAVFGLFLVNNVLPDRDRTAAAAAAIKAHVP
                     EITEGGWHNYTSLVASYPQEHPELTEELSATLGARWQEALPLIGYHGTVNPEQVLPAV
                     MRNSLPSGLRGFVLVAMLAAMMSTLAGQVNSVGSAMFVRDIYQNVFRPRANNRELIIA
                     SYAATAAIVGIGFYMGVSARSINHLWGWIMMGFGAGALAPKFLRLYWWRCTAWGCAAG
                     LALGGVSAIVQRLLWPEMVEWKQFLLTGGIGVAGVIVGSWCSAPPDRDIVRRFYRQTR
                     PFGLWKPFWNELTPTEQVGVRKEHRNDLAALPFTMVAQIALFMLSMQIV"
     gene            complement(2117..>2321)
                     /locus_tag="VEA63_08630"
     CDS             complement(2117..>2321)
                     /locus_tag="VEA63_08630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD84107.1"
                     /translation="GKCRAAGVAVGGQAGELRGGGGLRRKIQFNHEWTRIDTNEIEFG
                     CRIGSEHQTVMHTIGPKAFLCLL"
BASE COUNT          520 a          829 c          604 g          368 t
ORIGIN      
        1 cacgatctgc atcgacagca tgaacaacgc gatctgcgcc accatcgtga acggcagcgc
       61 cgcgagatca ttgcgatgct ccttccgcac gcccacctgc tcggtcggcg tcaattcgtt
      121 ccaaaacggt ttccacaaac caaacggccg cgtctgccga taaaaccgtc gcacgatgtc
      181 gcggtccggc ggcgcgctgc accacgaccc gacgatcacg cccgccacgc cgatgccgcc
      241 ggtcagcaaa aactgtttcc actccaccat ctcgggccac agcagccgct gcacgatcgc
      301 gctcacgccg ccgagcgcca gcccggccgc gcaaccccac gccgtgcacc gccaccaata
      361 cagccgcaga aatttcggcg cgagcgcacc ggcgccgaaa cccatcatga tccagcccca
      421 cagatgatta atgctccgcg ccgacacgcc catgtagaaa ccgattccga cgatcgccgc
      481 cgtcgccgca tacgacgcga tgatcaactc gcggttgttc gcgcgcggcc gaaacacgtt
      541 ttgataaatg tcccgcacga acatcgccga gcccacgctg ttcacctgac ccgcaagcgt
      601 ggacatcatc gctgccagca ttgccaccaa cacaaaaccg cgcagccccg acggcaggct
      661 gttgcgcatc accgccggca aaacctgctc cgggttcacc gtgccgtgat agccgatcaa
      721 cggcagcgcc tcctgccaac gcgcgcccag cgttgccgaa agttcctccg tcaactccgg
      781 atgctcctgc ggataactcg cgaccaaact cgtgtagttg tgccagccgc cttccgtgat
      841 ctccggcacg tgcgccttga tcgccgccgc cgcggcggcc gtgcgatcgc gatccggcag
      901 cacgttgttc acgagaaaca aaccgaagac cgcgaagccc atcatcatcg gccagcgaaa
      961 cgccaccgtc acgccctgca tcagcgaaag cagtccgcaa tcgcgatcgc tgcgcgcggc
     1021 gaaatagcgt gcatcgtcgc ccgacccggc gccgtcgaaa atgctcttca ccagataaaa
     1081 tcctgcgaac aacagcagcg actcatacgc ttcgtaaccc gccggcatcg tcgtgtgcac
     1141 cgccggcatc gcactcatcc actccgcatt gcccgtcacc cgctccgcca acaccgcgag
     1201 cgtcgccgcg tccggcacga gattgaacgc cagcgccgcc acgatcagac acgtcacgac
     1261 gataatgatg ccctgcacca gatcgttcac gatcacgccg tagaagcccg cgatcatcgt
     1321 gtagagcgtc gagaacgcga tcacgatcac cgtcgccgcc atcggcgaca acgggagaaa
     1381 aacgcccagg aacagcgacg tgccgcgcac cagatacgcg agcatcccga tcgtgaacac
     1441 gatcccgaac accgcgctga ttacccgcaa cgcctccgcc gccctgcccg ctccgaaacg
     1501 aaacacatac cattcgggtc ccgtcatgca cttcgagcgc cgataccatt tgcccgtcca
     1561 caacagcatg aacgcgagca ccaggaccgc gccaccgcga aactcgatga acaacccgcg
     1621 cggtccgagc atgaacagga acgacgtgat gatcatcgtg cccgtcagat cgaaccagtt
     1681 cgccatcccc gaaatcccga gcaacggcca cggcatccgc cgcccgccaa ggtagtagtg
     1741 ctcgaggctc tgcgtcgcac gtttgcgcat ccacaaaccg aacaggagcg tggcggcgag
     1801 atagacgccg acgatgacga gatcagcagg tcttaaagcg gacatgggga gttagggtga
     1861 agaaacggcg gacaacgcga gcattgcggt cgaacgacga tgtcagacta ccagacgcag
     1921 cggacccact acttgttttt atttgagcac taattgcgct tttttatctg cgcagcggag
     1981 cccgactcgc cgcaggtgga acccgacgtc ccgtcgggtt gcgttcgaaa tgaaggccgg
     2041 aaagccaggc ccgcacatcc gcttagcgga ttcatgcagt tgggaaaacc acgaatgaac
     2101 accactgaac acgaactcag aggaggcaga ggaaagcttt tggaccgatc gtgtgcatca
     2161 ccgtttggtg ttccgatccg attctgcatc cgaactcgat ttcattcgtg tcgattcgtg
     2221 tccattcgtg gttgaactga attttccgtc ttagcccgcc gcctcccctc aactcgccag
     2281 cttgtccgcc gaccgcgacg ccggcggcac gacatttccc g
//
LOCUS       DASPLT010000830         1067 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_70280, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000830 DASPLT010000000
VERSION     DASPLT010000830.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1067)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1067)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1067
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_70280"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            444..857
                     /locus_tag="VEA63_08635"
     CDS             444..857
                     /locus_tag="VEA63_08635"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HYD84108.1"
                     /translation="MLHNEVGSPKRLLRILYADDMRELREVARIALSREGHLIECVGD
                     GQEALQRVTADPNAYDLVISDHHMPHLNGIELVKRIRTLPYAGKILIFCSELSPAVSE
                     TYHQLKVDRILYKPVFPSELRQVLADLFTPTTCSP"
BASE COUNT          213 a          357 c          276 g          221 t
ORIGIN      
        1 ggcggtgcct gcgcagccac tcgtgagaag aacgccaccg gcgccgagcg cgaggacggc
       61 aatgagtttt agagaggaag ttttcatagg ttgacgactg ttagtgtcgc cgcacgccac
      121 gcggttcatc cgcggcgcac gattaacgct cttccgtcga tgccgcgggc gggccaactg
      181 aagttggtga acactcccgc gccgcaaaag cgtcacgcga actctctccg ccgcagtcac
      241 aacggcatgc gccgcaccca tcgtcgcgag cccgcgcgtc ctctctgaaa gccgtccgcg
      301 cgaccgccac gcgtcgggct aaacccgatg cgcacctacg ctttttttcg cagaaatttc
      361 gcagaactga gcggtgaaat cgcagtcact ccatgtgggt tcaccaatac ccgcttcgct
      421 tcatttttta tttcgtgctc acgatgcttc acaacgaagt cggctctcca aaacgactcc
      481 tgcgtattct ctacgcggac gacatgcgcg aactgcgcga ggtcgcacgt attgcgctca
      541 gtcgcgaggg tcatctcatc gaatgcgtcg gagacggcca ggaagcgctg caacgcgtca
      601 ccgccgatcc taacgcctac gatctcgtga tcagcgacca tcacatgccg cacttgaacg
      661 gcatcgaact ggtgaaacgg attcgcacgc tgccttacgc cggaaaaatt ttgatcttct
      721 gctcggagct cagccccgcc gtcagcgaga cgtatcatca actcaaggtc gaccgcatcc
      781 tctacaagcc ggtcttcccg tccgagctgc gccaggtcct cgccgatctc ttcacgccga
      841 cgacttgctc gccgtagtca aaattctaat acgcacaaaa gtagggcggg cttcagcccg
      901 ccttttttgt gcccattccc caagctcccg cctcgaagga accgcgcctt cgcttctcct
      961 caatgtttcc cctggagctt gaaggttgga gcttggatgt tggagcttcc ccacgctcac
     1021 gccgccggat ccaacacgac cttgatgcaa ccgtcgcgtt tctcgac
//
LOCUS       DASPLT010000831         1415 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_153330, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000831 DASPLT010000000
VERSION     DASPLT010000831.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1415)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1415)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1415
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_153330"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..851)
                     /gene="fliF"
                     /locus_tag="VEA63_08640"
     CDS             complement(<1..851)
                     /gene="fliF"
                     /locus_tag="VEA63_08640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009507946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body MS-ring/collar protein
                     FliF"
                     /protein_id="HYD84109.1"
                     /translation="MKNFTQSLLDLWKQLGLNQRVSLVVAALAVIAGLVGVALWSQRP
                     DYQLLYARLSEKDAAAIIGQLQTQNIPHVVTAGGTAVQVPSDQVYKLRMDLASKGLPS
                     GDGVGFEIFDKGQFGLSDFVQRTNYLRAVQGELSRTIMQVHGVRAARVMIVQPENRLL
                     LTDQGVKSTASVFVDVGGGRLDLDQVNAIRHLVANAVQGLAADQVAVIDNRGRTLSEE
                     LKQDPTLGSASSQMRYRQQVEDYFAKKVESMLAAVIGPGNAVVRVSAEIETEATTLTA
                     ERFDPES"
     gene            complement(913..1245)
                     /gene="fliE"
                     /locus_tag="VEA63_08645"
     CDS             complement(913..1245)
                     /gene="fliE"
                     /locus_tag="VEA63_08645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007363330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HYD84110.1"
                     /translation="MTPVGTVSPLHANPLTRIDAPLPKTSLTPSGPLTAAPADGFGQV
                     LDGIVSTVTSKQEASQVLTRKVLLGESDQLHQSVIAMQEASVAFTMMVEVRNKLVESY
                     QELMRMQV"
BASE COUNT          267 a          401 c          484 g          263 t
ORIGIN      
        1 tggctctcgg gatcgaagcg ctcggcggtg agagtggtcg cttccgtttc gatctcggcg
       61 gaaacgcgaa cgaccgcgtt gccggggccg atgacggcgg cgagcatgct ttcaactttc
      121 ttcgcgaagt agtcttcgac ctgctggcgg taacgcatct gcgacgacgc gctgcccaac
      181 gtgggatctt gtttcaattc ctccgaaagc gtgcggccgc gattgtcgat gacggcgacc
      241 tgatcggccg cgaggccctg gaccgcgttg gccacgaggt gacggatggc gttgacctgg
      301 tcgagatcga gccgcccgcc gccgacgtcg acgaacaccg acgcggtgga cttcacgccc
      361 tggtccgtga gcaatagtcg attttccggc tgcacgatca tcacgcgcgc ggcgcgcaca
      421 ccgtgcacct gcatgatggt gcgcgagagc tcgccctgga ccgcgcgaag atagttcgtg
      481 cgctgcacga aatcggagag gccgaactgg cccttgtcga aaatttcgaa gccgacgccg
      541 tcgccgctgg gcaggccctt cgacgcgagg tccatgcgca gcttgtagac ttggtccgag
      601 ggaacttgca cggcggtgcc gccggcggtg accacgtgcg ggatgttttg cgtctgcagc
      661 tggccaatga tggcggcggc gtctttttcg ctgagccgcg cgtagagcaa ttggtaatcc
      721 gggcgctgcg accagagcgc gacgccgacg aggccggcga tcaccgcgag cgcggcgacg
      781 accagcgaaa cgcgctggtt gagcccgagc tgtttccaca gatcgagcag ggattgcgtg
      841 aagtttttca tcagacgaga cgccggtgca gagaagaatt aagagaagcg gaaaaaagag
      901 ggaggtgagc actcatacct gcatgcgcat cagctcctgg taggattcca cgagcttgtt
      961 tcgcacttcg accatcatgg tgaaggcgac agatgcttcc tgcatcgcga tcacgctttg
     1021 gtgcaactgg tcggactcgc cgaggaggac cttgcgcgtc agcacctgcg aggcttcctg
     1081 cttcgacgtc accgtggaaa caattccatc gagcacctgg ccaaaaccgt cggcgggtgc
     1141 cgccgtcagc ggaccggacg gcgtgagcga ggtcttcggc aggggcgcgt cgatccgcgt
     1201 gagcggattg gcgtgcaaag gagaaacggt gccgacgggg gtcatgagtg agagaagctg
     1261 agagctgaga gtctagagct gagagcagga aggtgggcga aaggtggtcg ggtttggcgg
     1321 ctcttagctc tgggctctaa gctctgagct ccgcgctaag catcagcgaa tggtcacttt
     1381 ccgatctgca gggtcttata cgccatctgg cgggc
//
LOCUS       DASPLT010000832         1166 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_153333, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000832 DASPLT010000000
VERSION     DASPLT010000832.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1166)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1166)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1166
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_153333"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1166
                     /locus_tag="VEA63_08650"
     CDS             <1..>1166
                     /locus_tag="VEA63_08650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD84111.1"
                     /translation="TSGTTVRTFTIRNLGPGNLTIGSVTVSPSTDFTLLHQPATSITP
                     MHAPIWSDGRTTFSIQFNPTATGARTATVSFTNSDGDEGSFSFQIRGTGAAATANPFK
                     TTFPRLAGIFYNKSYSDTAYQQRIAKRDLAVLSFDKGWGGGGSTMLPANAAVNAIKAI
                     NPNILLGQYTLLESQYDHGPSTPAIDDIIDKLYAENWWILDGSGNKVTELYYNNMFMT
                     NFTSYTTTDANGDRYPEYLAKRHNTEIVDPISGLDFWFSDNAFYLPRKDVDWQQNGGA
                     DTDAVTLHEYRLAMASYWAKINQLQPGHFVLGNSDGQPSDKLGFLRDPEYQRKLQAAQ
                     FETALGASSSPEGYSGWYNMMTAYRSQLDNTLAPHLVIIGALSTATDGKAGHVG"
BASE COUNT          251 a          367 c          349 g          199 t
ORIGIN      
        1 accagcggca cgaccgtgcg cacgttcacg atccgcaacc tcggacccgg caacctgacg
       61 atcggcagcg tcacggtgtc gccgagcacc gatttcacgc tgctgcacca gccggcgaca
      121 agcatcacgc cgatgcatgc accgatctgg tcggacggcc gcacgacgtt tagcattcag
      181 ttcaacccga ccgcgaccgg agcgcgcacc gcgacggtga gtttcaccaa cagcgacggc
      241 gacgagggct cgttctcgtt tcagattcgc ggcacgggcg cggcggcaac ggcgaatccg
      301 ttcaagacca cgttcccccg actcgcggga attttctaca acaagagcta cagcgacacc
      361 gcctaccagc agcggatcgc gaaacgggat ctggcggtgc tcagcttcga taaaggttgg
      421 ggcggcggcg gctcgacgat gctgcccgcc aatgccgccg tgaacgcgat caaggcgatc
      481 aatccgaata tcctcctcgg ccagtatacg ctgctcgagt cgcagtatga tcacggtccg
      541 agcacgccgg ccatcgatga catcatcgac aagctctacg cggagaactg gtggatcttg
      601 gacggctccg gcaacaaggt cactgagctt tattacaaca acatgttcat gacgaacttc
      661 acgagctaca cgacgacgga cgcgaacggc gaccgttatc ccgaatatct cgcgaagcgg
      721 cacaataccg agatcgtcga cccgatttcg ggtctggatt tctggttttc cgacaacgcg
      781 ttctacctgc cgcgcaagga cgtcgactgg cagcagaacg gcggtgccga cacggacgcc
      841 gtgacgctgc acgagtatcg gctggcgatg gcttcgtatt gggcgaaaat caaccagctc
      901 cagccgggcc acttcgtcct gggcaattcg gacggacagc cttccgacaa actcgggttc
      961 cttcgcgatc ccgagtatca acgtaagctg caggcggcgc agttcgagac ggcgttgggc
     1021 gcgtcgagtt cgccggaagg ctacagcggc tggtataaca tgatgacggc gtatcgcagc
     1081 cagctcgata atacgctggc gccccatctc gtgatcattg gagcgctgag cacggcgaca
     1141 gacggcaagg cgggtcacgt cggcag
//
LOCUS       DASPLT010000833         1577 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_256034, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000833 DASPLT010000000
VERSION     DASPLT010000833.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1577)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1577)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1577
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_256034"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..348
                     /gene="mraW"
                     /locus_tag="VEA63_08655"
     CDS             <1..348
                     /gene="mraW"
                     /locus_tag="VEA63_08655"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009513264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytosine(1402)-N(4))-methyltransferase"
                     /protein_id="HYD84112.1"
                     /translation="ATRAFQGIRIAVNNEIGAIERALPAAFAKLVQPSSERAEGGVLA
                     VISFHSLEDRPVKQFFRRMCGQPESAADSTPQDFRVKLAEPLTRRPITPADDEIAANP
                     RSRSAKLRALRKL"
     gene            459..848
                     /locus_tag="VEA63_08660"
     CDS             459..848
                     /locus_tag="VEA63_08660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD84113.1"
                     /translation="MNLSSSHGFVNQLVAYTLVMIGFSGSIGLGTVWMRHQISVTANS
                     TKLLESRIAEVERHLVEASTAAETERDPSILLRRNEQWQLGLVAPDRTQIVHVAEDPV
                     MRLAAKRNRGLFGDAAPTISLPIALGR"
     gene            854..>1577
                     /locus_tag="VEA63_08665"
     CDS             854..>1577
                     /locus_tag="VEA63_08665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HYD84114.1"
                     /translation="MSKGFASNYRIVLLATGVVACFCGIGVRLVFLQVIDREELVRSI
                     EKVRRQIIVEHARRGDILDAKGNVLATSRSLIVLGVDPQTLRKEDEEKWPELARLIGM
                     PFGEMSRVFTTKTRPAKSGAKTGTEVRATAKFAIFDPASNPSAQAAKEVEEPATGGGA
                     GRAETEDDTQVDEIPDENGERSIRWAKLREDVSESTYAKILALGVKGVYGNRVYRRAY
                     PHNSLAAHMVGFVNKTGEPAAGI"
BASE COUNT          288 a          460 c          560 g          269 t
ORIGIN      
        1 gcgacgcgcg cgtttcaggg cattcgcatc gcggtgaaca acgagatcgg cgcgatcgag
       61 cgcgcgctgc cggcggcgtt cgcgaagctg gttcaacctt cgagcgaacg cgcagagggc
      121 ggggtgctgg cggtgatcag ctttcactcg ctggaggatc ggccggtgaa gcaatttttc
      181 cggcggatgt gcgggcagcc ggagagcgcg gcggattcca cgccgcagga ttttcgcgtg
      241 aagctcgccg agccgttgac ccgccgtccg atcacgccgg ccgacgacga gatcgccgcg
      301 aacccccgca gccgctccgc gaaattgcgc gcgttgcgga aactctgaag gacgacgatg
      361 cgcctcgaaa aatttccagc gaaagccagc gcgccgagaa ccacgcgctt cacccgcgac
      421 gccgagtgcg atcgcgcaac gctcaatccc cgctccagat gaatctttcc tcctctcatg
      481 gttttgtgaa ccagctcgtg gcctacacgc tggtgatgat tggctttagc ggctcgatcg
      541 gtctcggcac ggtgtggatg cgtcaccaga tttcggtgac ggcgaacagc acgaagctcc
      601 tcgagtcgcg gatcgccgag gtcgagcgtc acctcgtcga ggcgtcgacc gcggcggaga
      661 cggagcgcga tccgagcatt ttgctgcgtc gcaacgagca atggcaactc gggctcgtgg
      721 cgccggaccg cacgcagatc gttcacgtcg ccgaagaccc ggtgatgcgg ctggcggcca
      781 agcgcaaccg cgggctcttc ggagacgccg ctcccactat ttcgctgccg atcgcgctag
      841 ggcgctgacg ccgatgtcga agggcttcgc ctccaattat cgcatcgttc ttctcgcgac
      901 aggagtcgtg gcgtgtttct gcgggatcgg cgtgcggctg gtttttctgc aggtcatcga
      961 tcgcgaggaa ttggtgcgga gcatcgagaa ggtgcgtcgg cagatcatcg tcgagcatgc
     1021 gcggcggggc gacattctgg atgcgaaggg caacgtgctc gcgacgagcc gttcgttgat
     1081 cgtgctcggc gtcgatccgc agacgctgcg gaaggaagac gaggagaagt ggccggagct
     1141 cgcgcggttg atcggcatgc cgttcggcga gatgtcgcgc gtgtttacga cgaaaacgcg
     1201 tccggcgaag tcaggcgcga agacggggac cgaggtgcgt gccacggcga agttcgcgat
     1261 cttcgatccg gcgagcaatc cgagcgcgca agcggcgaag gaagtcgaag agccggcaac
     1321 gggcggcggc gcgggccggg ctgaaaccga ggacgatacg caggtggacg agattccgga
     1381 cgaaaacggc gagcgttcga ttcggtgggc gaagttgcgc gaggacgtga gcgaatcgac
     1441 ttacgcaaag attttggcgt tgggtgtgaa gggcgtgtat ggcaaccgcg tttaccgccg
     1501 tgcgtatccg cacaactcgc tcgcggcgca catggtgggc ttcgtgaaca agaccggcga
     1561 gccggcggcg gggatcg
//
LOCUS       DASPLT010000834         1922 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_174503, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000834 DASPLT010000000
VERSION     DASPLT010000834.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1922)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1922)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1922
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_174503"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1427)
                     /locus_tag="VEA63_08670"
     CDS             complement(<1..1427)
                     /locus_tag="VEA63_08670"
                     /inference="COORDINATES: protein motif:HMM:NF017197.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF748 domain-containing protein"
                     /protein_id="HYD84115.1"
                     /translation="MRSRNRRRLQLVAVLVALYALLGFFGLPPLIKWQLEKRLPEELG
                     RPVTIGRVRVNPFALSLGLERVTVRARDGSDTFLGWNRLHVNFDAIGSITDHWSLDEL
                     SLEDLRATVVVRPDGSLNFSDLIEKYRTPADADPKNAEPSRPWRVRRLNVASAAVAFA
                     DHSQADPFRTEFGPVNFTVMDFRTAGLRGAPGRFEAVTESGEKFTWIGSLTAAPLTSS
                     GDLRIENLALPKYAPYLVEFVRADVASGQLALDASYELDLSPQTRRVVLKSGAVELKD
                     LRVLQAGGGQALIELGVFAVSGIAGDALAAKVAVDQVRLQGGRVIATRSGDGSIDLLS
                     LLVPRETTTGENAPRPDVNVREIAVQGVQVQWRDESTPRPAELSFTDVNATLRNFTLA
                     PATAMPVELSLEWSPRGTLSASGNLTVNPLLADLDVKAANIALPPVSPYAEQFAQLGI
                     ASGTATLNGHVRVALAEDRPDLTFTG"
     gene            1690..>1922
                     /locus_tag="VEA63_08675"
     CDS             1690..>1922
                     /locus_tag="VEA63_08675"
                     /inference="COORDINATES: protein motif:HMM:NF021049.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiD C-terminal domain-containing protein"
                     /protein_id="HYD84116.1"
                     /translation="MIPLAKAMGVGVEVSDAKALVLTAPKEQNKNSLNTAFGGSLVSI
                     ATLAGYGVVWELMKDDTAPAKAEWRIVVKESRAA"
BASE COUNT          383 a          645 c          563 g          331 t
ORIGIN      
        1 cccgtaaacg tgaggtcggg ccgatcttcc gcgagcgcca cccgcacgtg cccgttgagc
       61 gtcgccgttc cgctcgcaat cccaagctgc gcgaactgtt ctgcatatgg actcaccggc
      121 ggaagcgcga tgttggcagc cttgacgtcg agatcggcca gcaacggatt cactgtcaga
      181 tttccggacg cgctcagcgt tccgcgcggg ctccactcca gcgaaagctc caccggcatg
      241 gcggtggctg gcgcgagcgt gaagttgcgc agcgtcgcgt tcacgtctgt gaaactcagt
      301 tcggcggggc gcggcgtgct ctcgtcgcgc cactgcacct gcaccccctg caccgcgatt
      361 tcccgcacgt tcacgtctgg gcgcggcgca ttttcccctg tggtcgtctc gcgcggcacg
      421 agcagcgaca acaaatcgat cgaaccgtca cccgagcgcg tcgcaatgac gcgtccgcct
      481 tgcagccgca cctggtcgac tgcgactttg gccgcgagcg cgtcaccggc gatcccgctc
      541 actgcaaaaa ctcccagctc gatcaacgcc tgtccgcccc cagcctgcag cacacgaaga
      601 tccttcaatt ccaccgcgcc gctcttcagc accacgcgtc gggtctgggg cgagagatcg
      661 agctcgtaag aagcatccaa cgcgagctgc ccggaggcca cgtctgcgcg cacaaattcc
      721 acgaggtagg gcgcatactt cggcagggcg agattctcga tgcggagatc accgctcgac
      781 gtcagcggcg cggccgtgag cgaaccgatc cacgtgaact tctcccccga ctccgtcacc
      841 gcttcgaagc gtcccggcgc gccgcgcaat cccgccgtgc ggaaatccat gaccgtgaaa
      901 ttcaccgggc cgaactccgt ccggaaaggg tccgcctgcg aatgatcggc aaacgccacg
      961 gccgcagagg cgacattcag ccggcgcacg cgccatggac gacttggttc cgcgttcttg
     1021 gggtccgcat cagcgggcgt tcgatatttt tcgatcaggt cggaaaagtt cagcgaaccg
     1081 tcgggccgca ccacaacggt cgcgcgcaaa tcttccagcg acagctcatc aagcgaccag
     1141 tgatccgtga tcgaaccgat cgcatcgaag ttaacatgca ggcggttcca cccgagaaat
     1201 gtatccgagc cgtcccgagc gcgcacggtc acccgctcca atccgagcga aagcgcgaag
     1261 ggattcacgc gcacgcgccc gatcgtgacc gggcggccga gttcttccgg caggcgtttc
     1321 tcgagctgcc atttgatcaa aggcggaagg ccgaagaatc cgagcagcgc gtaaagagca
     1381 accagcaccg caacaagttg gaggcgccgt cgatttcgtg atctcatgag agtgacggtt
     1441 gaaatgacac ggacaagcga atcgcccttc cgctccccca agattttagc aatgtccacg
     1501 ctcgtagcgg catcgctgtc gccggcgatt aacgattcgt tcacgtggcg taacttcccg
     1561 cgggaacata ggatactacc ggatcgcgaa accagacgcg tccgctttga cgccgctctt
     1621 tttttccctc aacttgcccg gctaatgaaa tcggtgaagg tccccgcctt cgaactcttc
     1681 ctccacgaga tgatcccgct cgcgaaagcg atgggcgtgg gcgtggaagt cagcgatgcg
     1741 aaggcgctcg tgctcaccgc cccgaaggag caaaacaaaa actccctgaa caccgccttt
     1801 ggcggcagcc tagtttccat cgcgacgctc gccggctatg gcgtcgtatg ggagttgatg
     1861 aaggacgaca ccgccccggc caaggccgag tggcgcatcg tcgtgaaaga aagccgcgcg
     1921 gc
//
LOCUS       DASPLT010000835         3067 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_49845, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000835 DASPLT010000000
VERSION     DASPLT010000835.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 3067)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3067)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3067
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_49845"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(145..783)
                     /locus_tag="VEA63_08680"
     CDS             complement(145..783)
                     /locus_tag="VEA63_08680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007362776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HYD84117.1"
                     /translation="MSKDKILQSLRQTKVIALIRADSPDSLVDCARALLAGGLNALEL
                     TMTTPGALEMTAKVAREVPELLLGLGTVLDVETARAGIAAGAKFIVTPAVRPTVIEVC
                     RELNVPILCGALTPTEAITASDLGADVIKIFPAEFFGPAYIRSLKAPFPKLEFLPTGG
                     VTPETVGAFLKAGAFATAAGSALVSPAALKAKDWAQITERARQFVAAANAPQ"
     gene            993..>3067
                     /locus_tag="VEA63_08685"
     CDS             993..>3067
                     /locus_tag="VEA63_08685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012377142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase 115 family protein"
                     /protein_id="HYD84118.1"
                     /translation="MCAFTLILSLPRAVHPAFRIRQLATAVLGLLALAAGSATYGAIG
                     EGAFVETQPVANGFTLVTKDRAAALHIDQQEFPGVLRIAGHLQADIERVTGVKPAIES
                     GVPRAPAVVIGTIGQGGLVDRLVASGKLDVAAINGKWDAFLIETVEAPFPGVERALVV
                     AGANKRGTIYGMYEISEQIGVSPWYWWADVPVKKREALYVKPGRVAEQVPVVQYRGIF
                     INDEAPALTGWVQEKFGKFDHTFYQHVFELILRLRGNYLWPAMWQPRAFIDDDPENAR
                     LADEMGVVIGTTHHEPMMRAHAEWDRYGKGPWDYSKNPEVLREFWRGGVERVKDKEII
                     VSLGMRGDGDEPMSEGENVALLERVVADQRTILGDVLGKKQEEIPQLWALYKEVQGYY
                     ERGMRVPDDVILLWCDDNWGNIRRLPTPEEQKRSGGAGVYYHFDYVGGPRNYKWINTT
                     PITKVWEQMNLAWQHDANRIWIVNVGDIKPMEFPIEFFLNMAWAPGRWNQDTLADFSR
                     AWAAREFGVEHAAEAAALVDGYTKLNGRRKPEMLAPDTLSLVNYREADRVLGEWRDLT
                     ARAEKLYGEIAPEARDAFFQLVLYPVKASGVVHEIHVAAGKNRLYAVQGRAAANTEAQ
                     RVRELFALDQELVKQYHSLGGGRWNHQMSQSKMGYTYWQTPNLEVAPAVSEVHPLAEA
                     SMAMGIEGS"
BASE COUNT          564 a          811 c         1137 g          555 t
ORIGIN      
        1 ggctgccgga tgtagggggt gcgaacgcgg atcggcccag agagccggga gcaggtaggc
       61 agggacgcct gccgctacgc ggagcgcgga caggcggccg cgccggtgaa gaacatcggg
      121 cgcctggttt tttggatgct cgatttactg cggtgcgttc gcggccgcga cgaattgccg
      181 ggcgcgctcg gtgatttgcg cccaatcttt cgccttcaag gcggcgggcg aaaccagcgc
      241 gctgccggcg gcggtcgcga aggcgccggc cttcaagaag gcgccgacgg tttccggcgt
      301 cacgccaccg gtcggaagaa actcgagctt cgggaagggc gctttcaacg agcggatgta
      361 agcgggaccg aaaaattcgg cggggaaaat tttgatcacg tcggcgccga gatcggacgc
      421 ggtgattgct tccgtcggcg taagcgcgcc gcacaggatc ggcacgttga gttcgcgaca
      481 gacttcgatc acggtggggc gcacggcggg ggtgacgatg aattttgccc cggcggcgat
      541 gccggcgcgg gcggtttcga cgtcgagcac cgtgccgagt ccgagcaaga gctcgggcac
      601 ttcgcgagcg acctttgcgg tcatctcgag tgcgccgggc gtggtcattg tcagctcgag
      661 cgcgttgagt ccgccggcga ggagtgcgcg ggcgcaatcg acgaggctgt cggggctgtc
      721 ggcgcgaata agcgcgatca ccttggtttg gcggaggctt tggaggattt tgtctttgga
      781 cataaaagaa ggggcggttg cgaaagaaca cccacggtga gcggcggatc tcgtttcctc
      841 aacgccggag tgcggagccg cgaataagcg gcagctcgag cttaacgcga cgaagttgtt
      901 cgactgtgag cgcagagcgc cggcccgcga gacttgccga tgccgtgacg ggcgggcacg
      961 ttcgcggaaa cgcaacccag cccctgttac ttatgtgcgc attcactctt atcctgtctc
     1021 ttcctcgtgc cgttcatccg gcgtttcgta tccggcaact ggcgacagcc gttctcggcc
     1081 tgctcgcact cgcggccggc agtgcgacct acggtgcgat cggtgagggc gctttcgtgg
     1141 aaacgcaacc ggtggcgaat ggttttacgc tcgtgacgaa ggatcgtgcg gcggcgttgc
     1201 acatcgatca gcaagagttt ccgggcgtat tgcgaatcgc ggggcacttg caggccgaca
     1261 tcgaacgggt gacaggcgtg aaaccggcga tcgagagcgg cgtgccgcgg gcgcccgcag
     1321 tggtgatcgg cacgatcggt cagggtgggt tggtcgatcg gctcgtggca tcgggcaagc
     1381 tcgacgtcgc ggcgatcaac gggaagtggg atgcgtttct gatcgaaacg gtggaggcgc
     1441 cgtttcccgg tgtagagcgc gcgttggtcg tcgcgggtgc gaacaagcgc gggacaattt
     1501 acggcatgta tgagatttcg gagcagattg gcgtgtcgcc gtggtattgg tgggcggacg
     1561 tgccggtgaa aaagcgcgag gcgctgtatg taaagccggg acgcgtggcg gagcaggtcc
     1621 cggtggtgca gtatcgcggt atcttcatca acgatgaggc gccggcgctg acgggctggg
     1681 tgcaggagaa attcgggaag ttcgaccaca cattttatca acatgtgttc gagctgatcc
     1741 tgcgtctgcg cggcaactac ctgtggccgg cgatgtggca gccgcgggcg ttcatcgacg
     1801 acgatcccga gaacgcgcgc ctcgcggatg agatgggcgt ggtgatcggc acgacgcacc
     1861 acgagccgat gatgcgcgcg cacgcggagt gggatcgcta cggcaaaggg ccgtgggact
     1921 attcgaaaaa ccccgaggtg ctgcgcgagt tctggcgtgg cggcgtcgaa cgcgtgaaag
     1981 acaaggagat catcgtttcg cttggcatgc gcggcgatgg ggacgagccg atgtcggagg
     2041 gcgagaatgt cgcgctgctc gagcgggttg tggccgatca gcgcacgatt cttggcgacg
     2101 tgctcggaaa aaaacaggag gagattcctc agctgtgggc gctctacaag gaagtgcagg
     2161 gctattacga gcgcggcatg cgcgtgcccg atgacgtgat tctgctctgg tgcgacgata
     2221 actggggcaa cattcgccgg ctgccgacgc cggaggagca gaagcgatcg ggcggcgcgg
     2281 gcgtgtatta tcatttcgac tacgtcggcg ggccgcggaa ctacaaatgg atcaacacga
     2341 cgccgatcac gaaggtgtgg gagcagatga atctcgcgtg gcagcacgac gcgaatcgca
     2401 tctggattgt gaacgtcggc gacatcaagc cgatggaatt tccgatcgag ttcttcctca
     2461 acatggcgtg ggcgcccggt cgttggaatc aggatacgct ggctgatttc agccgggcgt
     2521 gggcggcgcg ggagtttggc gtggagcatg cggcggaggc ggcggcgctc gtcgatggtt
     2581 acacgaagtt gaacggtcgg cggaagccgg agatgctcgc gccggatacg ctaagcctcg
     2641 tgaactatcg ggaagccgac cgcgtgctcg gcgagtggcg cgatctcacc gcgcgcgcgg
     2701 aaaagcttta tggcgaaatc gcacccgagg cgcgcgatgc gtttttccag ctcgtgcttt
     2761 atccggtgaa ggcgagcgga gtcgtgcacg agatccatgt cgcggcggga aagaaccggc
     2821 tttacgcggt gcaaggtagg gctgccgcga atacggaggc gcagcgggtg cgcgagttgt
     2881 tcgcgttgga tcaagagctc gtgaagcagt atcactcgct cggcggcggg cggtggaatc
     2941 accagatgtc gcagagcaaa atgggttaca cttactggca gacgccgaac ctcgaggtgg
     3001 cgccggcggt gagcgaggtg catccgttgg cggaggcgtc gatggcgatg ggcatcgaag
     3061 gttcgga
//
LOCUS       DASPLT010000836         1404 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_235179, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000836 DASPLT010000000
VERSION     DASPLT010000836.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1404)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1404)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1404
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_235179"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(114..578)
                     /locus_tag="VEA63_08690"
     CDS             complement(114..578)
                     /locus_tag="VEA63_08690"
                     /inference="COORDINATES: protein motif:HMM:NF020387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RES family NAD+ phosphorylase"
                     /protein_id="HYD84119.1"
                     /translation="MSSLVVWRLCAAKYASTAFDGEGARRYGGRWSPKGFRVNYTAES
                     RALAVVEVLAQVDERTRLVDLPWVLISAEFPESLVEKPARYPTDWRQFPHSRATQAFG
                     ESWLKESCTAALRIPSVVVPGEFNYLLNPTHPDFSRVKLAKPEPFTFDPRLA"
     gene            complement(575..991)
                     /locus_tag="VEA63_08695"
     CDS             complement(575..991)
                     /locus_tag="VEA63_08695"
                     /inference="COORDINATES: protein motif:HMM:TIGR02293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin Xre/MbcA/ParS toxin-binding
                     domain-containing protein"
                     /protein_id="HYD84120.1"
                     /translation="MKPPKRKPAVYDVIDDNHAMAQRVEEGLPVAEVVQFAKHAGFTN
                     DELARLIQIPPRTYARRIAARGRLKVTEGERAVRLMRLFERAKQVFGSDENTRGWFNG
                     QILALGGKTPLEFAQTEPGAREVENLIGRIEHGVFS"
     gene            complement(1216..>1404)
                     /locus_tag="VEA63_08700"
     CDS             complement(1216..>1404)
                     /locus_tag="VEA63_08700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHHA1 domain-containing protein"
                     /protein_id="HYD84121.1"
                     /translation="GAALGDRASLVVFCSPAAIKAGHQAGKIVGELSTKIGGKGGGKP
                     DFAMGGGKDPAKLAEVLK"
BASE COUNT          244 a          375 c          543 g          242 t
ORIGIN      
        1 aaggcggcga agatcgatcc gctggaggcg ttgcggacgg attgagtggg gcggtgcggg
       61 cggcggggga gcgggctgaa gcccgcccta cgttgtgcgt tgcaaagggg agattacgcg
      121 aggcgggggt cgaaggtgaa cggctcgggc ttggcgagtt tcactcggct gaaatccgga
      181 tgcgtggggt tgagcaagta gttgaattcg cccggcacga cgacgcttgg aatgcgcagg
      241 gcggcggtgc acgattcctt gagccatgat tcgccgaaag cctgggttgc gcgcgagtgg
      301 ggaaactggc gccaatcggt tggatagcgg gcgggttttt ccacgagcga ttcgggaaac
      361 tcggcgctga tcagcaccca cggcagatcg acgaggcggg tgcgttcgtc gacttgcgcg
      421 aggacctcga ccacggcgag cgcgcgcgat tcggcggtgt agttgacgcg aaaacctttg
      481 ggcgaccagc gtccgccgta gcgtcgcgcg ccttcgccat cgaacgcggt tgacgcgtat
      541 ttcgcggcgc agaggcgcca gacgacgagc gaactcacga aaagacgccg tgctcgatgc
      601 gaccgatcag attctcgacc tcgcgtgcgc cgggctcggt ctgcgcgaac tcgagaggcg
      661 tcttgccgcc gagggcgagg atctggccgt tgaaccaacc gcgggtattt tcgtcgctgc
      721 cgaaaacctg tttggcgcgt tcgaagagcc gcataaggcg gacggcgcgc tcgccctcgg
      781 tgactttgag gcggccgcgg gcggcgatgc ggcgagcgta ggtgcgcggg ggaatctgga
      841 tgaggcgggc gagctcgtcg ttggtgaagc cggcgtgctt ggcgaactgg acaacctcgg
      901 cgacgggcaa accctcttcc acgcgttgcg ccatcgcgtg gttatcgtcg atgacatcgt
      961 agacggcggg cttgcgcttc ggcggcttca tgatgaaggg acgataggcg agagggaggg
     1021 agaagcaagg gagaagtgcc aaatggcagc gcgatgagtg ccatttggcg tgggagacag
     1081 atgtcgtgaa gcgcggaggg aacgcgggaa taaggcggag cggcggatgg gagcagaatt
     1141 tcggacgcct cggagaggcg tccctaccgg cgctgccgcg cttgggaggc gcagcggcgt
     1201 ggaaatgacc gcgggctact tcaagacttc ggcgagtttg gcgggatctt tgccgccgcc
     1261 catggcgaag tcgggtttgc cgccgccttt gccgccgatt ttcgtgctga gttcgccgac
     1321 gatcttgccg gcttggtgtc cggctttgat ggcggcgggc gagcaaaaca cgacgagact
     1381 cgcgcggtcg ccgagggcgg cgcc
//
LOCUS       DASPLT010000837         1162 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_49867, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000837 DASPLT010000000
VERSION     DASPLT010000837.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1162)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1162)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1162
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_49867"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1032
                     /locus_tag="VEA63_08705"
     CDS             <1..1032
                     /locus_tag="VEA63_08705"
                     /inference="COORDINATES: protein motif:HMM:NF013791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HYD84122.1"
                     /translation="FSLEGFAFFTEAIFLGIFLYGWKRIPPLAHWFAGVMVAFSGILS
                     GIFVVTANAWMNAPTGFDFADGQFFNIDPIAAMFNRASLHQTLHMTLAAFVATGFGVA
                     GIHAWLLLRHPHSEFHRRALKIGALLGCVSIPLQIASGDLAARRVAELQPAKLAAMEA
                     HYHTRTHAPFVIGGIPDDTTQTTRYGVEIPGALSLLIGHSLDTEVPGLDQIPRDEWPN
                     VRLVHWSFDLMIACGLAMLALAAWSAWNAWRRRPLADARRLLRAFVLATPLGFIAIEA
                     GWMVTELGRQPWIVYGYMRTKDAVTDMPHLAVPFFTIAFVYLLLTVVVIVLMRRQFLD
                     TAPAVPPQR"
BASE COUNT          180 a          450 c          293 g          239 t
ORIGIN      
        1 ttttcgctcg aagggttcgc cttcttcacc gaggcgattt ttctcggcat ctttctctac
       61 ggctggaaac gcattccgcc actcgcgcat tggttcgccg gcgtcatggt cgcgttcagc
      121 ggaatcctct ccggcatctt cgtcgtcacc gccaacgcct ggatgaacgc gcccaccggc
      181 ttcgatttcg ccgacggcca gtttttcaac atcgatccga tcgccgcgat gttcaatcgc
      241 gccagtcttc accagacgtt gcacatgacg ctcgcggcat tcgtcgccac cggcttcggc
      301 gtcgccggca ttcacgcgtg gctgctcctg cgccatccgc acagcgagtt ccaccgccgc
      361 gcgctgaaaa tcggcgcact cctcggctgc gtttccatcc cgcttcaaat cgcctccggc
      421 gatctcgccg cccgccgcgt cgccgaactc cagcccgcga aactcgccgc gatggaagct
      481 cactaccaca cgcgcacgca cgcgcccttc gttatcggcg gtatccccga cgacactacg
      541 caaaccaccc gctacggcgt cgagattcct ggcgccctca gcctcctcat cggccacagc
      601 ctcgacaccg aggtcccggg tctcgaccaa atcccgcgcg acgaatggcc caacgtccgc
      661 ctcgttcatt ggtcgttcga tctcatgatc gcctgcggcc tggccatgct cgcgctcgcc
      721 gcttggtccg cttggaatgc ctggcgccga cgcccgctcg ccgacgcccg ccgcctgctg
      781 cgcgccttcg tcctcgccac gccgcttggc tttatcgcga tcgaagccgg ctggatggtc
      841 accgaactcg gacgccagcc ctggatcgtc tacggttaca tgcgcaccaa ggacgccgtc
      901 accgacatgc cgcacctcgc cgtgccgttc ttcaccatcg cgtttgtcta cctgctgctg
      961 acggtcgtcg tgatcgtgct aatgcgtcgc cagtttctcg atacagcccc cgccgtcccg
     1021 ccccagcgct gaaccgaact tccggatccc acgaaggcac gcgatctccc tgcaacgcat
     1081 ttaaccgcta atttccgcta atttttgact gataccgttc acgccacgcg cttcgccgat
     1141 caggcgaagc acgcaccgct tc
//
LOCUS       DASPLT010000838         1629 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_49872, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000838 DASPLT010000000
VERSION     DASPLT010000838.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1629)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1629)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1629
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_49872"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            69..1007
                     /locus_tag="VEA63_08710"
     CDS             69..1007
                     /locus_tag="VEA63_08710"
                     /inference="COORDINATES: protein motif:HMM:NF014376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HYD84123.1"
                     /translation="MISPFPPAPTEHLEWLGNVQEVRHPLSQSHPIWVRSSVVAAGAP
                     VPQPSVPSPEQHPYCEVAFVREGRGWQYIGSEKLERNSGDIILLGPGLPHYGMHLDWP
                     QRHSVVYFLPTVVFEMGPEGDGARVLERFTLPQPIRRRILRPPLELRAKLEAVFDDLQ
                     NEFDGSAVCREFRLRSLLAEILSSILRWDDRFHRDTATDRQAVNWVQIEKALRYIQEH
                     YTEAIYVQDVARAAGLGASRLQTMFREALGISCMQYLLSFRVSHAAASLCQPGARVAD
                     VAMATGFDALSNFNTAFRRAMGMSPTEYIRRRQHGA"
     gene            complement(934..>1629)
                     /locus_tag="VEA63_08715"
     CDS             complement(934..>1629)
                     /locus_tag="VEA63_08715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD84124.1"
                     /translation="VADPAKPVVISEFHTGDGAFAVLPHRNALYLAGYGPGSSVIAVD
                     IADPRHPRIADRDYDAAELQGTCALAVEGDTLYAVAYNAGTVSLYDVSDPLRLKRLSR
                     FRHDAMRGPGRISVRDGTAYVISSTNDTLAALDVTNPSRPTPRYFVQDARIKMPYGLA
                     VDGRYVYLAARQASSFVVLDRRKLEGANGANPLSRRRVSRRTFSPARVRPHADAAGCI
                     RSATCPSRGGRRC"
BASE COUNT          281 a          570 c          494 g          284 t
ORIGIN      
        1 gtttcgctcc ggctcgagca gcccgcgcct ccccgacggt cccaggaacc cccgcctcca
       61 cgccgcccat gatttctccg tttccacccg cccccaccga gcatctggaa tggctcggca
      121 atgttcagga agtccgccat ccgctgagtc agagccaccc catctgggtc cgctcctctg
      181 ttgtcgccgc cggcgcgcca gtgccgcaac cttccgtccc ttctccggaa caacacccgt
      241 attgcgaagt cgccttcgtt cgcgaaggcc gcggctggca atacatcggc agcgaaaaac
      301 tcgagcgaaa ttccggcgac atcatcctgc tcggtcccgg tctgccgcat tacgggatgc
      361 acctcgactg gccgcagcgg cactcggtgg tctacttcct ccccacggtc gttttcgaaa
      421 tgggccccga aggcgatggc gcccgcgtgc tcgaacgttt cacgctgccc caacccattc
      481 gccgccgcat tctccgcccg ccgctggagc tgcgcgcgaa actcgaagcg gtctttgacg
      541 atctgcagaa cgaattcgat ggaagcgccg tttgccgcga attccgcctg cgctccctgc
      601 tcgccgaaat cctctcgtcg atcctgcgct gggacgaccg ctttcaccgc gatactgcga
      661 ccgaccggca ggccgtcaac tgggtgcaaa tcgagaaagc gctccggtat atccaggaac
      721 attatacgga agcgatctac gtgcaggacg tcgcccgtgc cgcaggcctg ggagcgagcc
      781 gcctgcagac gatgttccgc gaggcgcttg gcattagttg catgcaatac ctgctctcgt
      841 ttcgcgtttc tcacgccgct gcgtcgctct gccagccggg cgcgcgggtc gcggacgtcg
      901 caatggcgac gggcttcgac gcgttgagca acttcaacac cgccttccgc cgcgcgatgg
      961 gcatgtcgcc gaccgaatac atccggcggc gtcagcatgg ggcctgacac gcgcgggact
     1021 gaaggtccga cgcgaaacgc ggcgccggct caaagggttc gctccattgg ccccctccag
     1081 ttttcgccga tcgagcacga cgaaactgct ggcctggcgc gcggcgagat acacgtagcg
     1141 gccatccacc gcgaggccgt agggcatctt gatgcgcgcg tcctgcacga aatagcgcgg
     1201 cgtcggacgc gaaggattcg tgacgtcgag cgccgccagc gtgtcgttcg tcgagctgat
     1261 cacgtaggct gtgccgtcgc gcaccgagat ccgtccgggt ccgcgcatcg cgtcgtgcct
     1321 gaaccggctg agccgtttca gacgaagcgg atcggacaca tcatagagcg acacggtgcc
     1381 cgcgttatag gcgactgcgt aaagtgtatc gccctcgacc gcgagcgcgc acgtgccctg
     1441 caactccgcg gcgtcgtagt cgcgatccgc gatccgcgga tgacgcggat cggcaatatc
     1501 gaccgcgatc accgaggaac ccggaccgta gccggcgaga tacaaggcgt tccggtgggg
     1561 cagcactgcg aaggcgccat cgccggtgtg aaactcggag atcaccaccg gcttcgccgg
     1621 gtccgcgac
//
LOCUS       DASPLT010000839         2008 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_133634, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000839 DASPLT010000000
VERSION     DASPLT010000839.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 2008)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2008)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2008
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_133634"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            257..643
                     /locus_tag="VEA63_08720"
     CDS             257..643
                     /locus_tag="VEA63_08720"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HYD84125.1"
                     /translation="MNDAPALLSGIAQIALASADPQKLVAWYRDTLGLPVLFEASGMT
                     FFQSGATRLMIGPAQPGQSVGGDTVIYFEPRDWSAAEAALEERGVVFLHPAQAVQRAP
                     GRELMLRPFRDPEGHALALMGWRAAN"
     gene            complement(673..1539)
                     /locus_tag="VEA63_08725"
     CDS             complement(673..1539)
                     /locus_tag="VEA63_08725"
                     /inference="COORDINATES: protein motif:HMM:NF024206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HYD84126.1"
                     /translation="MRSAPAIGGILFALSACTHPLAIHSNRMLGATAIEMFGEPRVAE
                     LADAACAGNVAGVGAAVADGVDPNSLSVGLDEFDGRVTPLLWAVDCGNTRGVEALLRA
                     GADPNQSTGGRVGLTPVLAAAESPNPAILRLLLQNAGNPDATTDAGAYTALMRAYQHG
                     RTLESVGREREAWLNWETLLDAGANPEATDGGRETVATFAATLNDFDKVIELLERGYS
                     YDLTYLGFWLQLDTEIPGMREPRFPIPPPSPESEASRQQAIAMLVQRGVRFPISREDV
                     GFRDPGVPANAN"
     gene            complement(1639..>2008)
                     /locus_tag="VEA63_08730"
     CDS             complement(1639..>2008)
                     /locus_tag="VEA63_08730"
                     /inference="COORDINATES: protein motif:HMM:NF016056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DCC1-like thiol-disulfide oxidoreductase family
                     protein"
                     /protein_id="HYD84127.1"
                     /translation="NSVIEVLYNGVCPVCRAGACDLERRAAAARAGVIFTDVAAHLDA
                     LTRAGVSLDDVRLKLHAITPDGRVLRGMPAVATAWAATPPYRTLGRLTQAPPFSWLAA
                     ALYHLTAHALWAWNRACGRW"
BASE COUNT          354 a          627 c          675 g          352 t
ORIGIN      
        1 gccgtgtcga gcaaatcagt tcgcgccatg aactcaattc tggtttgaca taattagttc
       61 gtcaaactaa atatatcggc gtccgctggc gtcgtcggca ggccgggttg cggtggtcga
      121 aacaccgctg ccggctggtt ctggttctcg ctttgcgagc ccggacgacg cgtagggatg
      181 aatcgatggc gacggcagga tgctggcggt cctcagatgt aatcctgcag cacgatgagg
      241 gatcgaggag agcgtgatga atgatgcgcc cgctttgttg tcagggatcg cgcagatagc
      301 cttggcgagc gccgatccgc aaaagctcgt ggcttggtat cgcgacacgc tgggcttgcc
      361 ggtgctgttc gaggcgtcgg ggatgacgtt cttccagtcc ggcgcgacgc ggctgatgat
      421 cgggccggcg cagccgggcc agagcgtcgg cggcgacacg gtgatctatt tcgagccgcg
      481 cgactggagc gcggcggaag cggcgctgga ggaacgcggc gtggtgtttc tgcacccggc
      541 gcaagcggtg cagcgcgcac cgggacgcga actgatgctg cgcccgttcc gcgacccgga
      601 gggacacgcg ctggcgctca tggggtggcg ggcggcgaat tgaggggtcg cgacagcggt
      661 cattgcctcc atctaattgg cattcgccgg cacaccggga tcacgaaatc ccacatcttc
      721 ccgactgatg ggaaagcgca cgccacgttg cacaagcatg gcaatcgctt gctgacgaga
      781 cgcttcagac tccggtgagg gcggcggtat ggggaaacgc ggttcgcgca tccctggaat
      841 ttctgtgtcg agttgcagcc agaagccgag gtaggtcagg tcgtacgaat aaccgcgctc
      901 caacaattcg atcactttgt cgaagtcgtt aagagtcgcc gcgaatgtcg cgactgtctc
      961 ccgcccacca tcggtggctt caggatttgc acctgcatcg agcagtgttt cccagtttaa
     1021 ccacgcctct ctctctcggc cgacggactc caaagtgcgc ccgtgttggt acgcccgcat
     1081 caacgccgtg tatgcgccag cgtcggtggt tgcgtcggga tttcccgcat tttgcagaag
     1141 aagtcggagg atcgccggat tcggggactc cgcagcagcg agcaccggcg tcagccccac
     1201 acgcccgccc gtgctctgat tggggtctgc tcccgcgcgc agcaatgcct cgaccccacg
     1261 cgtattcccg caatcgaccg cccagaggag tggcgttacg cgaccatcga actcgtccaa
     1321 gccgacgctt agtgaatttg gatcgactcc gtcggcgacc gctgcgccga cgcccgccac
     1381 attccccgcg catgccgcat ctgctagctc agcgacgcga ggctccccga acatctcaat
     1441 cgcagttgcg ccgagcattc ggtttgaatg gatcgcgagc ggatgggtgc acgcgctgag
     1501 cgcaaaaaga atcccgccaa tggcaggcgc actgcgcatc tgcccctccc tctcggctgc
     1561 cccagcgatc atcctcgccg agccggccct tttggcaaga ccgcgtcggg ccagaacgag
     1621 acgccaagct aaacgtgctc accaccgccc gcacgcccta ttccacgccc agagcgcatg
     1681 cgcggtgagg tgatagagcg cggcggcgag ccacgagaac ggcggcgctt gcgtgaggcg
     1741 gccgagcgtc cggtagggcg gcgtagcggc ccaggcggtg gcgacggcgg gcatgccgcg
     1801 gaggacgcgg ccgtccgggg tgatggcgtg cagtttcagc cgaacatcat cgagcgagac
     1861 gccggcgcgg gtgagcgcgt ccagatgggc ggcgacgtcg gtgaagatga cgcctgcgcg
     1921 cgccgcggcg gcgcggcgct ccagatcgca tgcgccggcg cggcagaccg ggcagacgcc
     1981 gttgtagagc acttcgatga cgctgttt
//
LOCUS       DASPLT010000840         1156 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_297182, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000840 DASPLT010000000
VERSION     DASPLT010000840.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1156)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1156)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1156
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_297182"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..789
                     /locus_tag="VEA63_08735"
     CDS             <1..789
                     /locus_tag="VEA63_08735"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070569 - uridylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase"
                     /protein_id="HYD84128.1"
                     /translation="VAQQKLDEQKEKMRESVRAALTNWARTVGEGADYTDNVPEQCLH
                     PVGHWFEIPNLLKNGTLARLLAAHPQLRTLVAHNIDTLGASLDPALVGWFQSSGATLG
                     WEVISKRIDDHGGGLARVDGRLRIVEGLALPREEDEFNLTYYSSNTCWIDIDKLLAVF
                     GLTRTDLADPELTAAAVRALAARVPTYVTLKDVKKRWGHGQEDIYPVAQFEKLWGDMT
                     ALPECTSAFTAVPRQRGQQLKDQAQLDAWQRDGSAAYVESLCDW"
BASE COUNT          219 a          408 c          329 g          200 t
ORIGIN      
        1 gtcgctcaac aaaagctgga cgagcaaaag gaaaaaatgc gcgagagcgt gcgcgctgcg
       61 ctcacgaatt gggcgcgcac tgtcggcgag ggcgccgact acaccgacaa cgttcccgag
      121 caatgcctgc acccggtcgg ccactggttc gagatcccca acctgctcaa aaatggcacg
      181 ctcgcccgcc ttctcgccgc gcatccgcag cttcgcacgc ttgtcgcgca taacatcgac
      241 acgctcggtg cctcgctcga tccggcactc gtcggctggt ttcaatcctc gggcgctacg
      301 ctcggctggg aagtgatctc gaaacgcatc gacgatcacg gcggcggact cgcccgcgtc
      361 gacggacgtc tgcgcatcgt cgaaggactc gcgcttccgc gcgaggagga cgaattcaat
      421 ctgacctact acagcagcaa cacctgctgg atcgacatcg acaagctcct cgccgtcttc
      481 ggcctcacgc gcactgatct cgccgatccc gaactcaccg ccgccgccgt ccgcgccctc
      541 gccgcgcgtg tgccgactta cgtgacgttg aaggacgtga aaaagcgctg gggccacggg
      601 caggaggaca tctatccagt cgctcagttt gaaaaactct ggggcgacat gaccgcactt
      661 cccgaatgca cgagtgcctt caccgccgtg cctcgccaac gcggtcagca gttgaaagat
      721 caagcgcagc tcgatgcctg gcagcgcgat ggctccgccg cctacgtcga atcactctgc
      781 gactggtaga cgcctccacc tcgaaggtgg agcccgactt ccgggcgggt cgtctcagac
      841 gccataacaa gtggggccgg cggtccccag ccggcctgag tttggcgatc gcacaacaag
      901 ggcgctgagg acacgcgccc ccaccctcga accgccctac atccgctctc ccaacctcct
      961 accacgtccg tctgattttt tgtttggcct cgcgcccgct cgggtcttca acacgcgcga
     1021 aaaatggcga tgcggttcag cattcttggc agcggcagtt ccggcaacgc cgcgctgatc
     1081 gtcacggacg aaacacgcgt gctcgtcgac gctggcttct ccgcgcgcaa gctcaattcg
     1141 ctgctcgccg ccgtgg
//
LOCUS       DASPLT010000841         2001 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_235228, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000841 DASPLT010000000
VERSION     DASPLT010000841.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2001)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2001
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_235228"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            15..137
                     /gene="ndhC"
                     /locus_tag="VEA63_08740"
     CDS             15..137
                     /gene="ndhC"
                     /locus_tag="VEA63_08740"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HYD84129.1"
                     /translation="MYREFLHNQIAILGPILFFLGVLVLGLFYEIKKGALEWER"
     gene            273..1613
                     /locus_tag="VEA63_08745"
     CDS             273..1613
                     /locus_tag="VEA63_08745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012377376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase"
                     /protein_id="HYD84130.1"
                     /translation="MRLDKIIARSRLVDLHSLDLEGALQELLAVCVKQFPDLKADALL
                     KGLLARESTMTTYLGLGVALPHVRIKMRRRYILAIGRSRAGIKHDGALSDERVRLIVM
                     LIAGEKARDYLQVLASIARQVKEPELVDGLVNAPSLDALHEQLVGGFGGIRPVQAQQN
                     RVNKLMFREAERVAHGASCRALMVFGDTFIGGIEAGALSTKLKTILVTRNPVDTGDDK
                     KEFTETIQVRSFSNQRMAQLRSAILVALTRGVIAFTDRVCCIGGMTGSNQFDTLTVVD
                     IEREFQTLLTGQNDLLPEDVKPEVLERVIAVATELAVEGREGRPVGCLFVIGDNDRVE
                     KLTKPLVLNPFFGYKEEDRNILNPFMDETVKEFSSIDGAFIIRGDGVVESAGSLIQAG
                     ESDFSLPSGLGSRHAAAAAISVAAHCISIVVSSSTGQVTLFRRGVMLPLTEKRR"
     gene            complement(1748..>2001)
                     /locus_tag="VEA63_08750"
     CDS             complement(1748..>2001)
                     /locus_tag="VEA63_08750"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF020034.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HYD84131.1"
                     /translation="EPHELFLANIQIHPAFQGNGLGSAVVRNVLESAAALQLSVRLQV
                     LKVNTRARDLYLRLGFTIYQETLTHFLMRHAATPARSET"
BASE COUNT          379 a          573 c          674 g          375 t
ORIGIN      
        1 tgccgtggac gttcatctat cgtgaattcc tgcacaacca gatcgcgatc ctcggcccga
       61 ttttgttttt cctcggcgtg ctcgtgctcg ggctgttcta cgagatcaag aagggcgcgc
      121 tggagtggga gcggtgagcc gcgcttcgca cggcaagatc caaccaccaa ggtccgagct
      181 ccagagaaac ttcaactttc aggataccat tggagctttc gagtttgaga tttgaatgga
      241 gcttggagct tgaagtttgg agcttctgat cgatgcggct cgataaaatc atcgctcgaa
      301 gccggctcgt cgatctgcat agcctcgacc tcgaaggcgc gctgcaggag ctgctcgcgg
      361 tgtgcgtgaa gcagtttccc gatttgaagg cggacgcgtt gctcaaaggc ctgctggcgc
      421 gcgagagcac gatgacgacg tatctcggcc ttggcgtggc gctgccgcac gttcggataa
      481 aaatgcgccg ccgctacatc ctcgcgatcg gccgcagccg cgcgggcatc aagcacgacg
      541 gcgcgctgag cgacgagcgc gtgcgcttga tcgtgatgct catcgccggc gagaaggcgc
      601 gcgattatct ccaggtgctc gcgtcgatcg cgcgccaggt gaaagagccg gaactcgtcg
      661 acggtctcgt caacgcgccg agtctcgatg cgctgcacga acagctcgtc ggcggctttg
      721 gcggaatccg gcccgtgcag gcccagcaga atcgcgtcaa caagctgatg tttcgcgagg
      781 ctgagcgcgt cgcgcacggc gcgagttgcc gcgcgctgat ggtgtttggc gacacgttca
      841 tcggcggcat cgaagccggc gcgttgtcga cgaagctcaa gacgatcctc gtcacccgca
      901 atccggtcga cacgggcgac gacaagaaag aattcaccga gacgatccag gtgcgatcgt
      961 tctcaaacca gcgcatggcg cagctgcgca gcgcaatcct cgtggcgctg acgcgcggcg
     1021 tcatcgcgtt caccgatcgg gtgtgctgca tcggcggaat gaccgggagc aaccagttcg
     1081 acacgctcac cgtcgtcgac atcgagcgcg agtttcagac gctgctcacg ggccagaacg
     1141 acctgttgcc cgaggacgtg aaaccggaag tgctcgagcg cgtgatcgcg gttgccaccg
     1201 agctcgcagt cgagggacgc gaaggtcggc cggtgggctg cctgtttgtc atcggtgaca
     1261 acgaccgggt tgaaaagctg accaagccgc tcgtgctgaa tccatttttt ggttacaagg
     1321 aggaggatcg caacatcctg aatccgttca tggacgaaac ggtgaaggag ttttcgtcga
     1381 tcgacggcgc gttcatcatt cgcggcgacg gcgtggtgga gtccgcgggc agtttgatcc
     1441 aggcgggcga gagcgatttt tcgctgccga gcgggctcgg cagccggcat gcggcggcgg
     1501 cggcaatcag cgtcgcggcg cattgcattt cgattgtggt gtcgtcgagc accgggcagg
     1561 tgacgctctt ccggcgaggc gtgatgctgc cgctgacgga aaaacgacgc tgagcgagcg
     1621 tggtgggctc gaccgcgcaa ccggtggggc tgcacgaaaa cgagcgggcg gtggagcccg
     1681 gcgtcccgcc gggcttgctt gaggatcgcg gcgaacaacc cgacaggccc gggttccgtc
     1741 tgcacaacta tgtttcgctg cgcgctggag tggcggcgtg acgcatcagg aagtgcgtca
     1801 gcgtttcctg gtaaatcgtg aagccgagtc gcaaatagag atcgcgggcg cgcgtgttga
     1861 ccttgagcac ctgcaggcgc acggaaagtt gaagcgcagc ggcggattcg agcacattgc
     1921 gcacaaccgc cgagccgagg ccgttgcctt gaaacgccgg gtgaatttga atgttcgcga
     1981 gaaacagttc gtgcggttca c
//
LOCUS       DASPLT010000842         1672 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_275763, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000842 DASPLT010000000
VERSION     DASPLT010000842.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1672)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1672)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1672
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_275763"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..494)
                     /locus_tag="VEA63_08755"
     CDS             complement(<1..494)
                     /locus_tag="VEA63_08755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta galactosidase jelly roll domain-containing
                     protein"
                     /protein_id="HYD84132.1"
                     /translation="MPRLLCAFLFSTLCVVIPHALAETAPINVQARAGLTLNGKWSTI
                     VDPYLNGYYNYRLQEHPNGYFMDRQQQDKSELLEYNFDQSPTLTVPGDWNSQEEKLLY
                     YEGAIWYRRKFDYRPAAGDSRLFVHFGAANYQADVYLNGKKVGRHVGGFTPFAFEVTG
                     NVKPT"
     gene            691..>1672
                     /locus_tag="VEA63_08760"
     CDS             691..>1672
                     /locus_tag="VEA63_08760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD84133.1"
                     /translation="MSTRFAPGLFSLVFLRHLVGCLSFVMFAQVGIAQDQTVPSGGTA
                     LLASELATTGGFYANDLGSGPIAERTIVNVAGQNFSQAARVATTQPAGEHYRSAVGAN
                     ATRSVADGDVVLLHFFMRAIQTTDESGQVTAQVYAQGPAPGYVSSMSETISAGPEWTE
                     FFVPFTVNGSHASGAFNINFGFGAPVRPQVLELGGVEAIWYGTSRTLAEMPRTAFQYD
                     GRAADAPWRAAAAARIEQYRKGSFTVNVVDGTGAPVAGAHVRVRQRRHAFEFGTAFVG
                     SRVLDQTDPNNAIYREKLLELFNAGSPENDLKWQPWIGEWGASFSPQIAQG"
BASE COUNT          304 a          446 c          600 g          322 t
ORIGIN      
        1 gtgggcttga cgttgccggt cacttcaaag gcgaacggcg tgaagccgcc gacatggcgt
       61 ccgactttct tgccgttgag atagacatcg gcctggtagt tcgcggcgcc gaagtgcacg
      121 aacaaacggc tgtcgcccgc ggcgggacga tagtcgaatt tgcgccgata ccagatggcg
      181 ccctcgtaat agaggagctt ttcctcctgc gaattccagt cgccggggac ggtgagcgtg
      241 gggctctggt cgaaattata ttcgagcagc tcggacttgt cctgctgctg ccggtccatg
      301 aaataaccgt ttgggtgctc ctggagccgg tagttgtagt aaccgttgag atagggatcg
      361 acgatcgtcg accatttgcc gttaagcgtc aggccggcgc gcgcctgcac gttgatcgga
      421 gcggtctcgg cgagggcgtg aggaatgacg acgcagagcg tgctgaagag aaaagcgcag
      481 agaaggcggg gcatgggagt gggtttaacg tggtgtgaac gagaaggaga gaggagaggc
      541 tgaggcgatg ctttatcgtg tcagctcttc gtcaccgcgg cctcttgaag tcggtcacgt
      601 gaagggctgg ctccataatg atagtaacaa gtttcccggc ggaaaacgaa gagactagcg
      661 ttccgaggcc cggcatccaa cgtgactccg atgtccactc gctttgcccc gggcttgttc
      721 tcgctcgttt ttcttcggca tctcgtggga tgcctctcct tcgtgatgtt tgctcaagtc
      781 ggcattgctc aggatcagac ggtgccgtcg ggcgggacgg cgttgctggc gtccgagttg
      841 gcgacgaccg gcgggtttta tgcgaacgat ctcgggagtg gcccgatcgc ggagcggacg
      901 atcgtcaacg tggcagggca aaacttcagc caggcggcgc gcgtggcgac gacgcagccg
      961 gcgggtgaac attatcggag tgcggtcggg gcgaacgcga cgcgcagcgt ggcggacggc
     1021 gacgtggtgt tgctgcactt tttcatgcgg gcaattcaga ccaccgacga atccggacag
     1081 gtcaccgcgc aagtgtatgc gcaggggccg gcgccgggct atgtcagttc gatgtcggaa
     1141 acgatttccg ccggaccgga gtggacggag tttttcgtgc cgttcacggt caacggcagc
     1201 cacgcgagtg gcgcgttcaa cattaacttc gggtttggcg ctccggttcg cccgcaggtg
     1261 ctcgagctcg gcggcgtgga ggcgatctgg tatgggacga gccggacgct ggcggagatg
     1321 ccgcgcacgg cgttccaata cgacggccgc gcggcggatg cgccgtggcg ggcggcggcg
     1381 gcggcgcgca tcgaacagta tcggaagggc agcttcacgg tgaacgtggt cgacggcacc
     1441 ggcgcgccgg tggcgggtgc gcacgtgcgc gtgcggcagc ggcggcatgc gttcgaattc
     1501 ggcacggcgt tcgtcggctc acgcgtcctc gatcagacgg acccgaacaa tgcgatttat
     1561 cgcgagaagc tgctcgagtt gttcaacgcg ggcagcccgg agaacgatct aaaatggcag
     1621 ccgtggattg gcgaatgggg agcgagcttt agtccacaga ttgcgcaagg cg
//
LOCUS       DASPLT010000843         1412 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_214387, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000843 DASPLT010000000
VERSION     DASPLT010000843.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1412)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1412)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1412
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_214387"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..590)
                     /locus_tag="VEA63_08765"
     CDS             complement(<1..590)
                     /locus_tag="VEA63_08765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HYD84134.1"
                     /translation="MPQLEVKIPPMGESISTGILAKWHVQNGDVVKKDQPLFELETDK
                     ITSEGTAEAAGKITFKAEAGAEVKIGEVVATIDTDAAAAPSPRDADSAAANTGKDATS
                     TSAVPNPYAPPQTEQAASAAGAGEKPIPEAAGAKSRATESSPAVRRIASETGIDPAGV
                     KGTGKAGRVTKGDILAAAAPSAPQSQISNLKSQIAAP"
     gene            complement(689..>1412)
                     /gene="sucA"
                     /locus_tag="VEA63_08770"
     CDS             complement(689..>1412)
                     /gene="sucA"
                     /locus_tag="VEA63_08770"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376991.1"
                     /note="SucA; E1 component of the oxoglutarate
                     dehydrogenase complex which catalyzes the formation of
                     succinyl-CoA from 2-oxoglutarate; SucA catalyzes the
                     reaction of 2-oxoglutarate with dihydrolipoamide
                     succinyltransferase-lipoate to form dihydrolipoamide
                     succinyltransferase-succinyldihydrolipoate and carbon
                     dioxide; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HYD84135.1"
                     /translation="DFVNGAQAIIDQFIASAESKWERMSGLVLLLPHGYEGQGPEHSS
                     ARLERFLQLCAEDNIQVVNITTPANFFHALRRQMKRDFRKPLVVMSPKSLLRHPAATS
                     RLEEFHTGTFQEVIDDPARPEKPERVIFCSGKVYYDLCDYRDRNKIAGTAIVRIEQLY
                     PLHTSRLSELADDYEGARLVWCQEEPQNMGAWTFIGPHLSRVFGTFPLYAGRDAAASP
                     AVGALALHKFELAAFLQDAFNI"
BASE COUNT          222 a          364 c          524 g          302 t
ORIGIN      
        1 ggcgcagcga tttgagattt caaatttgaa atttgagatt gcggcgccga tggcgccgcg
       61 gcggcgagga tgtcgccctt tgtgacgcgg ccggctttgc cggtgccctt gacgccggcc
      121 gggtcgatgc cggtttccga agcgatgcga cgcaccgcgg gcgacgattc ggtcgcgcgc
      181 gattttgcgc cggcggcttc ggggattggc ttttcgccag cgccggcggc ggaggcggct
      241 tgttcggttt gcgggggcgc gtagggattg ggaacggcgg aggtggaggt ggcgtccttg
      301 ccagtgttgg cggcggcgga gtcggcgtcg cgcggcgagg gcgctgcggc ggcgtcggtg
      361 tcgatggtgg cgacgacttc gccgattttc acttccgcgc cggcttcggc tttgaacgtg
      421 attttgccgg ccgcttcggc ggtgccttcg gaggtgatct tgtcagtctc gagctcgaag
      481 agcggctggt cttttttgac gacgtcgccg ttttggacgt gccacttggc aaggatgccg
      541 gtgctgatgg attcgcccat cggcgggatt ttgacttcga gttggggcat gggagagaga
      601 agtttggctc gttctcgtaa tcgttctctt tctcgttctc gcggtttgtc ggaacggttc
      661 gagaacgaag aacgagaacg atactgagtt aaatgttgaa ggcgtcctga aggaaggcgg
      721 ctagttcgaa tttgtggagg gcaagcgcgc cgacggcggg cgaggcggcg gcgtcgcggc
      781 cggcgtagag cgggaaggtg ccaaacacgc gcgagagatg cgggccgatg aaggtccacg
      841 cgcccatgtt ttgcggctct tcctggcacc agacgaggcg cgcgccttcg tagtcgtcgg
      901 cgagttcgga gaggcggctc gtgtgcaacg gataaagctg ctcgatgcgc acgatcgccg
      961 tgccggcgat tttgttgcgg tcgcggtaat cgcagaggtc gtagtagacc ttgccgctgc
     1021 aaaagataac gcgctcgggt ttttcgggac gcgccggatc gtcgatgact tcctggaacg
     1081 tgccggtgtg aaactcctcg aggcgggacg ttgcggcagg atggcgcagg agagatttcg
     1141 gcgacatcac gacgagcggc ttgcggaaat cgcgtttcat ctgtcggcga agcgcgtgaa
     1201 agaaattcgc gggcgtggtg atgttgacca cctgaatgtt gtcctcggcg cagagttgga
     1261 ggaagcgctc gaggcgcgcg gaggaatgct ccggaccctg gccttcgtag ccgtgcggga
     1321 gcaggagcac gagcccgctc atgcgctccc acttcgattc cgcgctcgcg atgaattgat
     1381 cgatgatggc ctgcgcgccg tttacgaaat ct
//
LOCUS       DASPLT010000844         3801 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_111709, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000844 DASPLT010000000
VERSION     DASPLT010000844.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 3801)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3801)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3801
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_111709"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1390)
                     /locus_tag="VEA63_08775"
     CDS             complement(<1..1390)
                     /locus_tag="VEA63_08775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007364235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HYD84136.1"
                     /translation="MAQAYTEASIKTLSSTEHIRLRPGMYIGRLGNGTHAEDGIYVLL
                     KESIDNSIDEFTMGHGKKIEVELNERTVRVRDYGRGIPLGKLLECVSVINTGAKYDSE
                     TFKKAVGLNGVGQKAVNALSVNYRAQAFREGQTKVVEFEQGKLKKDAKVVKTDERNGT
                     LIEFTPDEALFGKDFKFRAEFIEDMLWNYAFLNRGLTLLFNGKVFRSENGLKDLLQKN
                     LSGEPLYPIVHLEGEDIEVAFTHGAHYGEEYYTFVNGQHTTMGGTHLAAFREALVETV
                     RNFFKRDYEAADVRQSIDAAIVVRVMEPVFESQTKTKLGSQDMAPGGPSIKEFVGKFM
                     QQSLDVYLHKNKEMAETLRGKIESSERERKELAGIRNLARERAKKASLHNKKLRDCRL
                     HLGDRNERAEESTLFIVEGDSAAGSLTATRDVQTQAVFALKGKPLNTFGLSKKVIYEN
                     EEFHLLQSALNVE"
     gene            1579..2334
                     /locus_tag="VEA63_08780"
     CDS             1579..2334
                     /locus_tag="VEA63_08780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD84137.1"
                     /translation="MKKILLFLVIALLAVVLIAYVAGQFFLGSAVEAGVNRVGPRITQ
                     TRVNLDDASLSPLNGTGTLRGLAVGNPAGWSEANAFYLGQVHLDVEPASVLGDTIVIN
                     NLVIDQPEITYETRVVSSNIGDLLKNIEASVGGGAEKPDDPNARPKKYIVKHFALQNG
                     KVTVGVGPAALPLPMPPLELNDLGVKEGGLTAGQLAVAVTRAVLPNVIAAAASAGGKL
                     GGALGGQAGDAVKEASEGLKKLFGGEKKKEPSK"
     gene            2506..3165
                     /locus_tag="VEA63_08785"
     CDS             2506..3165
                     /locus_tag="VEA63_08785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012372936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein DsbA"
                     /protein_id="HYD84138.1"
                     /translation="MKITYYLEMLSSWCTWAEPTWAELKKRYAGRAEFHWKIALMNPQ
                     DFPVSREQCDWFYRRSGGTVMHSPVMLNSGWFEPERKGHYEAADLVAEAGRDFGFNGD
                     EIRLALNHAAMFEGRKVGDMAVSVAIAAQAGNLDATALRTRAESPEVRARVDASTKEF
                     FAHQINQRPSFILEDAIGDKAVFSGLVRLEPLIATIDTMLADTAAYAAHKAHFGAPPS
                     H"
     gene            complement(3366..>3801)
                     /locus_tag="VEA63_08790"
     CDS             complement(3366..>3801)
                     /locus_tag="VEA63_08790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HYD84139.1"
                     /translation="RLSTGLPASELRKWRANAITHYTFKRDGVLKGWGIGGAARWQDE
                     IVIGFPVYRDPVLGLVQDIRRPFMGSDELKFDTWLSYERPLFDKKVVWKVQFNIRNLL
                     NDDLLIPVRANPVAVDDYTNYTIGAYRIGEARTFELTSSFAF"
BASE COUNT          688 a         1386 c          997 g          730 t
ORIGIN      
        1 cctcgacgtt caacgccgac tgcaacaggt gaaactcctc gttctcgtag atcactttct
       61 tcgagagccc aaacgtgttc agcggtttcc ccttcaacgc gaacaccgcc tgcgtctgca
      121 cgtcgcgcgt cgccgtcagc gagcccgccg ccgaatcgcc ttcgacgatg aacaacgtgc
      181 tttcctccgc ccgctcgttg cgatcgccga gatgcagccg gcaatcgcgc agtttcttgt
      241 tgtgcagcga cgccttcttc gcgcgctcgc gcgcgagatt ccgaatgccc gcgagctcct
      301 ttcgctcgcg ctcgctcgac tcgatctttc cgcgcaacgt ctccgccatc tccttgtttt
      361 tgtggagata cacgtcgagc gattgctgca tgaacttgcc gacgaactcc ttgatcgacg
      421 gtccgcccgg cgccatgtcc tgcgaaccga gcttcgtctt cgtctgcgat tcgaacaccg
      481 gctccatcac gcgcaccacg atcgccgcat cgatcgactg ccgcacgtcc gccgcctcgt
      541 agtcgcgctt gaaaaaattc cgcaccgtct cgaccagcgc ttcgcgaaac gccgcgaggt
      601 gcgtgccgcc catcgtcgtg tgctgcccgt tcacgaacgt gtaatactcc tcgccgtagt
      661 gcgcgccgtg cgtgaacgcc acctcgatgt cctcgccctc gaggtgcacg atcggataca
      721 gcggctcgcc gctcaaattt ttctgcaaca ggtccttcaa cccgttctcc gagcgaaaca
      781 ccttgccgtt gaacaacagc gtcagcccgc gattcaaaaa cgcgtagttc cacaacatgt
      841 cctcgatgaa ctccgcgcga aacttgaagt ccttcccgaa cagcgcctcg tccggcgtga
      901 actcgatcag cgtgccattg cgctcgtcgg tcttcaccac tttcgcgtcc ttcttcaact
      961 tcccctgctc gaactcgacg accttcgtct gcccttcgcg aaacgcctgc gcgcgataat
     1021 tcaccgacaa cgcgttgacc gccttctgac cgacgccgtt caatcccacc gctttcttga
     1081 acgtctcact gtcgtatttt gcaccggtgt tgatgaccga cacgcactcc agcaattttc
     1141 cgagcggaat gccgcgcccg taatcccgca cgcgcacggt ccgctcgttg agctcgacct
     1201 cgatcttctt gccgtggccc atcgtgaatt cgtcgatcga gttgtcgatc gactccttca
     1261 acagcacgta gatgccgtct tcggcgtgag tgccgttgcc cagccgcccg atatacatgc
     1321 cggggcgcag gcggatatgc tcggtcgatg aaagggtctt gatgctcgct tcagtgtaag
     1381 cttgggccat gatttttggt gcgaaaacac agcgccgcgc gccgctcgct gacaagcgca
     1441 attgcaagta gggtggcgct tcagcccgcc ctctccacac aaccttcttc cgtccgccac
     1501 gaacccgccc accctcccga ggtccaacgc tcacccactc ccgccgcggt tcttccgctc
     1561 agccactcac ttctcctcat gaaaaaaatc ctcctcttcc tcgtcatcgc cctgcttgcc
     1621 gtcgtcctca tcgcttacgt cgccggccag ttcttcctcg gctccgcggt cgaagccggc
     1681 gtcaaccgcg tcggcccgcg catcacgcaa acccgcgtga acctcgacga cgcctccctc
     1741 tcgccgctca acggcaccgg cacgctccgc ggcctcgccg tcggcaatcc cgccggttgg
     1801 agcgaagcga acgcgttcta cctcggccag gtgcatctcg acgtcgaacc cgcctccgtc
     1861 ctcggcgata cgatcgtcat caacaacctc gtgatcgacc aacccgagat cacctatgag
     1921 acgcgcgtcg tctcgagcaa catcggcgac ctgttgaaga acatcgaagc gtccgtcggc
     1981 ggcggcgcgg aaaaacccga cgaccccaac gccaggccga aaaaatacat cgtgaaacat
     2041 ttcgcgctcc agaacggcaa ggtcaccgtc ggcgtcggtc ccgccgcgct ccctctcccg
     2101 atgccgccgc tcgagctcaa cgatctcggc gtgaaggaag gcggcctcac cgccggccaa
     2161 ctcgccgtcg ccgtcacgcg tgccgtgcta cccaacgtca tcgccgcggc cgcctccgcc
     2221 ggcggcaaac tcggtggcgc tctcggaggc caggccggcg acgccgttaa ggaagcgagc
     2281 gaaggcctca aaaaactctt cggcggcgaa aagaaaaaag agccgtcgaa gtgaccccta
     2341 tgtagggcgg gcttcagccc gcctttccgt caaaacgggt ttaaaacccg ccccaccccg
     2401 caccgctcgc ggccgtcgcg tgtgttcacc tcgccgacac accgctgtgc cttccaccgc
     2461 cccctcctcc gttgacgccg tcctccgccc cgcttcgctg ctcgcgtgaa aatcacctac
     2521 tatctcgaaa tgctttcgtc ctggtgcacg tgggctgaac cgacttgggc cgagctgaaa
     2581 aaacgctacg ccggccgcgc ggaatttcac tggaagatcg cgctgatgaa cccgcaggat
     2641 ttccccgtat cgcgcgaaca atgcgactgg ttctatcgcc gcagcggcgg caccgtcatg
     2701 cactcgccgg tcatgctgaa ttccggctgg ttcgagcccg aacgcaaagg ccactacgaa
     2761 gccgccgatc tcgtcgccga agccggtcgc gattttggtt tcaacggcga cgagatccgt
     2821 ctcgcgctca accacgccgc gatgttcgaa ggccgcaaag tcggcgacat ggccgtctcc
     2881 gtcgccatcg ccgcccaagc cggcaacctc gacgccaccg ccctccgcac gcgcgccgaa
     2941 tcccccgaag tccgcgcccg cgtcgacgcc agcacgaagg aatttttcgc gcaccagatc
     3001 aatcagcggc cgtcgttcat cctcgaagac gcgatcggcg acaaagccgt tttctccggc
     3061 ctcgtccgcc tcgaaccgct gatcgcgacg atcgacacga tgctcgccga cacggccgcc
     3121 tacgccgcgc acaaagccca cttcggcgca ccgccgtcgc attaaggtgg ggcgcgttat
     3181 ccctaacgcg ccgattcgag ccgcccttct ctttcgtcgc tcacccgggg cgcgttaaga
     3241 ataacccgcg ctatctcccg cctcgttggt ctcacaccgt ttcggagggg gcaagagctc
     3301 cgcgtctcgt ttgggtagcg gcaggcctcc gggcctgccg ctacgtttcg actctgacgc
     3361 tctgctcaaa acgcgaacga cgaagtcagc tcgaacgtcc gtgcctcgcc gatgcgatac
     3421 gcgccgatcg tgtaattcgt gtagtcgtcc accgccaccg gattggcgcg aaccggaatg
     3481 agcaggtcgt cattgagcag attgcggatg ttgaactgca ctttccaaac aaccttcttg
     3541 tcgaacagcg gccgctcgta gctgagccag gtgtcgaact tgagctcgtc ggaccccatg
     3601 aacggacggc gaatatcctg caccagcccg agcaccggat cgcgatagac cgggaagccg
     3661 atcacgattt cgtcctgcca ccgcgcggcg ccgccgatgc cccacccttt cagcacgccg
     3721 tcgcgcttga acgtgtaatg cgtgatcgca ttcgcgcgcc atttgcgcag ttccgacgca
     3781 ggcaaacccg tggagaggcg g
//
LOCUS       DASPLT010000845         1334 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_133683, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000845 DASPLT010000000
VERSION     DASPLT010000845.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1334)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1334)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1334
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_133683"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1334)
                     /locus_tag="VEA63_08795"
     CDS             complement(<1..>1334)
                     /locus_tag="VEA63_08795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD84140.1"
                     /translation="GRWEVQATAGEGGRIDAICDLGHGVVVFATRKPNPGKLYLSENY
                     GETWAPLPSPTGNAITCLTAADRNRFYLLTDHAEVFGTHDGGRTWKALRGAMPNRSRV
                     GAAAAYGLMLTPRGTLLMSDTDSDGGHLYRSTDDGETWSDLGIVSPDALYRFMRVGNG
                     IVLNGFAGAVYKSVDDGRTWNRQQQLSASALFATEFLGGAFALQADQAGRVYRSSNLG
                     ETWEQVAALDGAADDFIDLGFGGVYYSTYTGDRDVYFSLNYGKDWLKLGPLPDSENGD
                     WLDHGIRLDAPDFIVAIGGTIKGRIVRNVIARDRLQEVTTVRRHGGTMEVPPDLRKDL
                     EVALVDRTVNFAELAEPEDVLLHAGHAYVPCRTGNNVAIFRLGDQGRAEWIGSIRDED
                     ILDAFGVAAEGGHLFVLSMSNATVTVFDVSDPTRPVKTGVVRVGGEGARLDTV"
BASE COUNT          237 a          453 c          405 g          239 t
ORIGIN      
        1 accgtgtcga gacgcgctcc ttctccgccc acgcgcacga cgccggtttt caccggccgt
       61 gtcggatcgc ttacgtcgaa aaccgtcacc gtcgcattgc tcatcgacag cacgaacaga
      121 tgcccgccct ccgccgccac gccgaaggcg tcgagtatat cctcgtcacg gatacttccg
      181 atccattcgg cgcgaccttg atcgcccagt cggaagatcg cgacgttgtt gccggtgcgg
      241 cagggcacgt aggcgtggcc agcgtgcagg agcacgtctt caggttcggc aagctcggca
      301 aagttgaccg ttcgatcgac gagcgcgacc tcgaggtctt tccgaagatc cggcggaacc
      361 tccatggttc ccccgtggcg gcgaacggtg gtgacttcct gcagccggtc gcgcgcgata
      421 acgttgcgca cgatccggcc tttgatcgtt ccgccgatcg ccacaatgaa gtccggcgcg
      481 tcgagccgga tgccgtgatc cagccagtcg ccgttttcgc tgtcggggag cgggcccagc
      541 ttcaaccagt ccttgccgta attcagagag aaatagacat cccgatcgcc cgtgtaggtg
      601 gaataataga cgccgccgaa accaagatcg atgaagtcgt cggccgctcc atcgagcgct
      661 gcgacctgct cccaggtttc gcccagattg ctgctgcggt aaacccgtcc ggcctggtcc
      721 gcttgcagcg cgaacgcgcc accgagaaac tcggtcgcaa acaacgccga ggcgctcaac
      781 tgctgctggc gattccacgt gcggccgtcg tcaacgcttt tgtagactgc gcccgcaaag
      841 ccgttcagca cgatgccgtt tccgacgcgc atgaaacgat acagcgcgtc cggcgaaacg
      901 attccgagat ccgaccaggt ttcaccgtcg tcggttgagc ggtagagatg tccgccgtcc
      961 gaatccgtgt cggacatcaa gagcgtgccg cgcggcgtga gcatcaagcc ataagccgcc
     1021 gcggcgccga cgcggctgcg attgggcatc gcgccacgga gcgctttcca ggtccgcccg
     1081 ccgtcatgcg tgccaaacac ctcggcatga tcggtcagca gataaaatcg gttccggtcc
     1141 gcggcggtga ggcacgtgat cgcgtttccc gtcggcgacg gcagcggcgc ccaagtctcg
     1201 ccgtagtttt cgctcaaata cagcttgccc gggttgggct tgcgcgtggc gaacaccacg
     1261 acgccatggc cgaggtcgca gatcgcgtcg atgcggccgc cctcgcccgc cgtcgcctgc
     1321 acctcccagc ggcc
//
LOCUS       DASPLT010000846         2870 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_214399, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000846 DASPLT010000000
VERSION     DASPLT010000846.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 2870)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2870)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2870
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_214399"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1523)
                     /locus_tag="VEA63_08800"
     CDS             complement(<1..1523)
                     /locus_tag="VEA63_08800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HYD84141.1"
                     /translation="MSDAHDQSGSLCGVWVDSAGSVHTTVARPDGSRETRLSTFRPFA
                     WMNGTPDAANTMAVQVEELPGEGPFNRLAHADDFGVYETFVRQARATVGVDPIRPLES
                     QFLLQQRERLYREMTFAQLRRCQMDIETASSDDGFCDPARPDDRVLAIGLRVGGRNRL
                     LVLEEMTDAAEKKLLEDFNVAIAEEDPDVIEGHNIFKFDFDYLRQRCRRFKVPCAWGR
                     YGQKATFRSSRLKVAERWIDFLRCDLAGRSVVDTYLLVQLYDITTRELTSYGLKDVAV
                     YFGITEEDSLTRTYIEGHRIKDVYREDRARFLAYLTDDLRETEGLAAQLLPTYFEQVR
                     TFPTLLQEATLRGATSKIDLLFLEEYYHARQACPLPPEVQPFEGGYTRSFQEGVFRHV
                     LHFDVASLYPSLLLQNGRHPKTDSLGVFIPLLRRLREYRLVYKQLARNAPTAEERAEA
                     QARQTNFKILINSFYGYLGFSGARFGDGELAAEVTRRGRELLQTLIDEFAKHGCTIL"
     gene            complement(1585..1923)
                     /locus_tag="VEA63_08805"
     CDS             complement(1585..1923)
                     /locus_tag="VEA63_08805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009514019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD84142.1"
                     /translation="MSPTFYYVLHVISVLVLTGYTFYAFAAPAETRKRVMMVTGIASL
                     LVLVGGFGLQAKLGYGFPGWLIVKMVCWLGLSALAGMGYRKRGAAGTLSVVALLIAAT
                     AVVMVYTKPF"
     gene            complement(2104..2285)
                     /gene="ssrS"
                     /locus_tag="VEA63_08810"
     ncRNA           complement(2104..2285)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="VEA63_08810"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(2387..2848)
                     /locus_tag="VEA63_08815"
     CDS             complement(2387..2848)
                     /locus_tag="VEA63_08815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007362464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD84143.1"
                     /translation="MKRIEVKLSLPVVAPLLDVIKGLSDALRTDLAAPLAMSDLDPEF
                     REAWAADLMEAQASDVGVLLALFDDEFFSEGVIALDGANAEPVLRACAAVRLRLREKY
                     LKGLGDETLESSEVELEQLDEPVRKAFMCYLFLATLQELIIQHLDSTILEA"
BASE COUNT          601 a         1003 c          745 g          521 t
ORIGIN      
        1 tcaagaatcg tgcagccatg cttcgcgaat tcgtcgatca gcgtctgcaa caattcccgt
       61 ccccggcgcg tgacctccgc cgccagctcc ccatccccaa atcgcgcgcc cgaaaaaccc
      121 agatagccgt agaatgagtt gatcaggatc ttgaaattgg tctgacgcgc ctgcgcctcc
      181 gcccgctctt ccgccgtcgg cgcgttgcgc gcgagctgct tgtaaaccag ccgatactcg
      241 cgcaaccgcc gtaacaacgg aatgaataca cccaggctgt ccgttttcgg gtgccgcccg
      301 ttctgcagca gcaaactcgg atacagcgac gccacgtcga aatgcagcac gtgacgaaac
      361 acgccttcct gaaaactccg cgtataaccg ccctcgaacg gctgcacttc cggcggcagc
      421 gggcaggcct ggcgcgcgtg ataatattcc tccagaaaca gcagatcgat cttcgaggtc
      481 gcgccccgca gcgtcgcttc ctgcagcagt gtcggaaacg tccgcacttg ctcgaaatac
      541 gtcggcagca actgcgccgc gagcccttcg gtctcgcgca aatcgtccgt cagatacgct
      601 agaaatcgcg cgcgatcctc gcggtaaaca tccttgatcc gatggccttc aatgtaagtg
      661 cgcgtgaggc tgtcctcttc cgtgatcccg aaatacaccg ccacgtcctt gagcccatac
      721 gacgtcagtt cgcgcgtcgt gatgtcgtaa agctgcacga gcaaatacgt gtcgaccacc
      781 gaccgccccg ccaaatcgca gcgcagaaaa tcgatccagc gctccgccac ctttagacgg
      841 ctgctgcgaa acgtcgcctt ctgcccatac cgcccccacg cgcacggcac cttgaaccgc
      901 cggcagcgct gacgcaggta atcgaaatcg aatttgaaga tgttgtgccc ttcgatcacg
      961 tccgggtctt cctcggcgat cgcgacgttg aagtcttcca acagcttctt ctccgccgcg
     1021 tccgtcatct cctcgagcac gagcaatcga ttccgcccgc cgacgcgcag tccgatcgca
     1081 agcacacgat cgtccggcct cgccggatcg caaaacccgt cgtcggacga tgccgtctcg
     1141 atgtccatct ggcagcgtcg caactgcgcg aacgtcatct cgcgatacag ccgctcgcgc
     1201 tgctgcagca agaattggct ctcgagcggc cggatgggat caaccccgac cgtcgcacgg
     1261 gcctgccgga cgaacgtttc gtaaacgcca aaatcgtccg cgtgcgccag ccggttgaac
     1321 ggcccttcgc ccggcaactc ctccacctgc accgccatcg tgttcgccgc gtccggcgtt
     1381 ccgttcatcc acgcaaacgg ccgaaacgtg ctcagccgcg tctcgcgtga accatccggc
     1441 cgggcgaccg tcgtgtgcac gctgcccgcg ctgtcgaccc acacgccgca cagcgaaccg
     1501 gattgatcgt gcgcatcgct catgccggga tcgtgatcgt cgtaggttgc gagctcgcct
     1561 cgcaacgaag cggtatcgcg ccgctcagaa cggcttcgtg taaaccatca ccaccgcagt
     1621 cgccgcgatc aacagcgcaa ccacgctcaa cgttcccgcc gcaccgcgct tgcggtagcc
     1681 catgccggcg agcgccgaga gaccgagcca gcacaccatt ttcacgatca gccagccggg
     1741 aaagccgtag ccgagtttcg cctgcaaccc gaagccgccg accagcacga gcaagctcgc
     1801 aatgcccgtg accatcatca ctcgcttccg cgtttccgcg ggcgcggcaa acgcgtaaaa
     1861 cgtgtaaccc gtcaaaacca ataccgagat aacgtgtaga acgtagtaga atgtggggct
     1921 catgaagaag aagcgtggaa accgtgcgac tgaaggcgat agctaactct gcccatcgtt
     1981 tgtttctctt cgctgtctat cttcgccttc tccataggcg tccgctgtag ggcccggcgc
     2041 tcgacaacgc gcgagctcgc agaagaagga tagcggagaa gaaacccgaa agaaaaaaaa
     2101 agacgacacc ccgcctgtgc cgtatgccca aaggctcagg acctttttag ttttaggtgg
     2161 gcgcctccgc cgcagcgtct gccttccgat ttacggcctg gcatcttccg cgacgggttc
     2221 agctcccaat tttattctaa ggcaaagagc agttattgaa agcacctcga acactgcagg
     2281 gtgtcggcgt ggaaagtagg tgcgacatcg gccccgtcaa atgccgattc cttttttcgc
     2341 gtagcggcag gcttccgcgc ccgccagctc tcggcgcggc ccaaattcac gcctccaaaa
     2401 tcgtcgagtc cagatgctga atgatcagct cctgcaacgt cgcgagaaac aagtaacaca
     2461 tgaacgcctt ccgcaccggc tcgtccaact gctccagttc gacctcgctg ctttccagcg
     2521 tctcatcgcc caaccctttc aaatattttt ctcgcaaccg caaccgcacc gctgcgcacg
     2581 cccgcaacac cggctccgcg tttgcgccat cgagcgcgat cacgccttcg gaaaaaaact
     2641 cgtcgtcgaa caacgccagc aacacgccga cgtcgctcgc ctgagcctcc atcaaatccg
     2701 ccgcccacgc ctcgcggaac tccgggtcga gatcgctcat cgccagcggc gccgccaggt
     2761 cagtgcgcaa cgcatccgac aatcctttga tcacgtcgag cagcggcgcg accaccggca
     2821 gactgagttt cacctcaatg cgtttcatcg gactcgagca ccgcagtcag
//
LOCUS       DASPLT010000847         1497 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_111734, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000847 DASPLT010000000
VERSION     DASPLT010000847.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1497)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1497)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1497
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_111734"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1254)
                     /locus_tag="VEA63_08820"
     CDS             complement(<1..1254)
                     /locus_tag="VEA63_08820"
                     /inference="COORDINATES: protein motif:HMM:NF013767.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HYD84144.1"
                     /translation="MSSDFSPELRKELLDDFYAECDELLTSVRQSLSALEQSLGSGEP
                     AASVVEALFRGVHSLKGIAAIAGVRPAELLAHGMEDLLRALSQRDVSLNVERLDVLLD
                     ATQRLEEIIRAHQAGKPAPDHEAALKTLRTAGRAKGKGAKKERETPAVFASASAAAKP
                     DAVADPLAEAQARGLKLWRCTFAPTAALDQRGVNVNCVRERLGQIGEILKAAPTVQPN
                     ASIVFVFTVGFREEPEDLAAWEADGIRFEPIVMEVEPSAAAPIPAAAPGVAEQKDALS
                     LMPSHMVRVDLARLDELMRITGEMVIQRSRLEDRIEQQCGGHESLKEIDQGLARSLRE
                     MRKAIARVRLVPIAEIFTRMPFVLRDLSTGSGKKARVALEGHQTEIDKYLAERLKEPL
                     LHLVRNAFSHGIETPAERATAGKPEE"
BASE COUNT          269 a          568 c          401 g          259 t
ORIGIN      
        1 ctcctcgggc ttgcccgctg tcgcgcgctc cgccggcgtt tcgattccgt gcgaaaacgc
       61 gttgcgcacg agatgcagca gcggctcctt cagccgctcc gcgagatatt tgtcgatctc
      121 ggtctgatga ccttcgagcg ccacgcgcgc cttctttccc gatccggtcg acagatcgcg
      181 caacacgaac ggcattcgcg tgaaaatctc cgcgatcggc accagccgca cgcgcgcgat
      241 cgccttgcgc atctcgcgca gcgaacgtgc cagaccctgg tcaatctcct tcaatgactc
      301 gtgtccgccg cactgttgct cgatccgatc ctccaagcgc gaacgctgaa tcaccatctc
      361 gcccgtgatc cgcatcaact catccaatcg cgcgaggtcc acgcgcacca tgtgcgacgg
      421 catgagcgac aacgcatcct tctgctccgc cacacccgga gcggccgcgg ggatcggcgc
      481 cgccgcgctc ggttccacct ccatgacgat cggctcgaac cgaatcccat ccgcttccca
      541 cgcggcgaga tcctccggct cctcgcgaaa tccgacagta aacacgaaca cgatcgacgc
      601 attcggctgc acggtcggcg ccgctttcaa aatctcgccg atctggccaa gccgctcgcg
      661 cacgcagttc acgttgacgc cccgctgatc gagtgccgcc gtcggcgcaa acgtgcaccg
      721 ccacaatttc aatcctcgcg cctgcgcctc cgccaacggg tccgccaccg cgtccggctt
      781 cgccgccgcc gatgccgatg caaaaaccgc cggcgtctcg cgctcttttt tcgcgccttt
      841 ccctttcgcg cgccctgccg tgcgcagcgt tttcaacgcg gcctcgtgat caggcgccgg
      901 ttttcccgcc tgatgcgcac ggattatctc ctcgagccgt tgcgtcgcat cgagcagcac
      961 atcgagccgc tcgacattga gcgacacgtc acgctgcgaa agcgcgcgca acaaatcctc
     1021 cattccatgc gcgagcaact ccgccggccg cacgcccgcg attgcggcga tccccttcag
     1081 cgaatgcacg ccgcgaaaca gcgcttcgac gacgctcgcc gccggctcgc ccgaccccag
     1141 cgattgctcc agcgccgaca aactctgccg caccgacgtc agcagctcat cgcactcggc
     1201 ataaaaatcg tccagtagct ctttgcgcag ctcgggtgaa aaatccgaac tcatgagcga
     1261 ccgcctccgg ccggcaacgc atcggcaacg ccgccgcgca aacgcgtgta accggcaaaa
     1321 atgagaaggg gcgtgcgagg catgggcgac ggcaccgcca acccatccgc agcggcacgg
     1381 ttggcaactt tcccgcgcgc tccgcctctg gcgagacgtt ttttcgctct atgatgttgg
     1441 tgccatgcga cacctgacgg aacgatgccg cagcgcctca acgcgcacgt cgatcac
//
LOCUS       DASPLT010000848         1442 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_195325, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000848 DASPLT010000000
VERSION     DASPLT010000848.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1442)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1442)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1442
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_195325"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1415)
                     /locus_tag="VEA63_08825"
     CDS             complement(<1..1415)
                     /locus_tag="VEA63_08825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD84145.1"
                     /translation="MAPLALPPGPFFEQLVANGLFGYTDVFNYHYYGYAEDFSDVYHQ
                     FGDAAASARNAETPTTEMRKTEPAYGEGPGRFGSNSSGFSFQDVSVSAFPAAPRPMRR
                     VPVMLSEFGYGRLGESARDTVEGRVRQWAWFRDVALQIRKERIAAPIAFRFRSTLERG
                     LYDFGLTRNADESLAVEGGADDGMRAAGVAFRPADFGAEEREPWMNTIGKRMGGAEAS
                     PALAWLTDYGERQRYRARDWKVRAPAASLMVIDFIADETLSQRKRYGGYLVMGGGDAA
                     HVSGTGTVVVYNFSNETVRGRLEGPLLAARAGKAEKLKSGNADIGKPTRWTGGGEEAW
                     PLELEMAPMARMTIPVRLELNAERFAGVRWALRFEPESRGIATADFSTVLYPSPAGMK
                     ETLVRRFDYPAEQGADARASLLTRPLAAEEPVSTEAGRWLVTEGVTVAEDTRGRWRFV
                     VEPSADPARQRVMAELPLSDD"
BASE COUNT          266 a          560 c          365 g          251 t
ORIGIN      
        1 aaatcgtctg acaacgggag ctctgccatc acccgttgac gcgccggatc ggcgcttggc
       61 tcgacgacga aacgccagcg tccccgagtg tcctccgcta cagtcacgcc ttccgtgacg
      121 agccagcgtc ctgcctccgt cgacaccggc tcctccgccg caagcggccg cgtgagcaac
      181 gacgctcgcg cgtccgcccc ttgctccgcc ggataatcga agcgccgcac cagcgtctct
      241 ttcattcctg caggactcgg ataaagcacc gtcgaaaaat ccgccgttgc gatcccccgc
      301 gactccggct cgaaacgcag tgcccatcgc accccggcaa accgctccgc attcaactcg
      361 agccgcaccg gaatcgtcat cctcgccatt ggcgccatct ccaactccaa cggccacgcc
      421 tcctcaccgc cccccgtcca ccgtgtcggc tttccgatgt cagcgtttcc gcttttcagc
      481 ttttcagctt ttcccgctcg cgcagcgagc aagggcccct cgagccgccc gcgcacggtc
      541 tcattcgaaa aattataaac caccaccgtg cccgtcccgc tcacgtgcgc cgcgtcgccg
      601 ccacccatga ccagatagcc gccgtagcgt ttccgttggc tcagcgtctc gtccgcgatg
      661 aagtcgatca ccatcaggga tgccgccggt gcccgaacct tccaatcgcg cgcccgatac
      721 cgctgccgtt cgccgtagtc cgtgagccat gccagcgccg gactcgcctc ggctccgccc
      781 atacgtttcc cgatcgtatt catccacggc tcccgctctt ccgcaccgaa atccgccggc
      841 cgaaaggcca ccccggccgc acgcatcccg tcgtcagcgc cgccctcaac cgccaagctt
      901 tcatctgcat ttcgcgtcag cccgaagtcg taaagccccc gctcaagcgt ggaacgaaac
      961 cgaaacgcaa tcggcgccgc gatccgctcc tttcgaattt gcagggcgac atcgcgaaac
     1021 cacgcccact gccgcacgcg cccttccacc gtgtcgcgcg cgctctcccc cagcctcccg
     1081 tatccaaatt ctgacaacat caccggcacc cgccgcatag gccgcggagc ggccggaaac
     1141 gcggaaacgc tgacatcctg aaagctgaaa cccgaggagt tcgatccgaa gcgtcccggc
     1201 ccttcgccat aagcgggttc cgttttccgc atttcagttg tcggcgtttc agcatttcgc
     1261 gccgacgccg ccgcgtcgcc aaattgatga tacacatcgg aaaaatcctc tgcgtagccg
     1321 tagtagtgat aattgaaaac gtccgtgtag ccgaacagcc cgttggcgac cagttgctcg
     1381 aaaaacggtc cgggcggcag cgcgagcggc gccatcgcga cccatggctg acgcgcgcga
     1441 cg
//
LOCUS       DASPLT010000849         1176 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_133705, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000849 DASPLT010000000
VERSION     DASPLT010000849.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1176)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1176)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1176
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_133705"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1176
                     /locus_tag="VEA63_08830"
     CDS             <1..>1176
                     /locus_tag="VEA63_08830"
                     /inference="COORDINATES: protein motif:HMM:NF019138.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HYD84146.1"
                     /translation="DSDRDDRAPDRERGDRETPPPERPPEPRPSDTGASGDTRASHDA
                     KPESSSSSNEAPPAPTPQPAAPSGGGGQGDWQERGGNRGGDRGGDRGDRGGGGGDGGG
                     RDFWKRNKRKRGRHGGGGGGGHWQPGGGGGHPREQHPPQPPPPSNAPIFGELPNPSRF
                     EDLAALETNANDISSGGGDPIFLNELYALNLADLTAFARNQGVAFEGAPNKKQLLSLV
                     LASAAEKKRPLKDRGYIDVTDRGAFIVHEHVNYRLYPEDPYLPESLVRLYGLKRGHRV
                     EVQVEAPRPGERCPSVVRVESVMGMPPADISKVTPFEELVPYYPLQRILLESEETQKD
                     VSMRAVDILTPIGFGQRALIVAPPRTGKTVLLQNIANSITANSPHAKLILLLIDERPE
                     "
BASE COUNT          225 a          431 c          351 g          169 t
ORIGIN      
        1 gacagtgacc gcgacgatcg cgcgccggat cgcgaacgtg gcgaccgcga aacgcctcca
       61 cccgaacgtc cgcccgaacc gcgaccctcc gacaccggag ccagcggcga cacgcgcgcg
      121 tcgcacgacg cgaagcccga atcttcttcg tcctcaaacg aggcaccgcc cgccccaact
      181 ccgcaaccgg ctgcacccag cggcggcggc ggccaaggcg attggcagga acgcggtggc
      241 aaccgcggcg gagatcgtgg cggcgatcgt ggcgaccgcg gaggtggtgg tggcgacggt
      301 ggcggtcgcg atttttggaa gcgcaataaa cgcaaacgcg gccgccacgg cggtggcggt
      361 ggcggcggcc actggcaacc cggcggcggt ggcggtcacc cgcgcgaaca acacccgccg
      421 caacctccgc ctccgagcaa cgcgccgatt ttcggcgaac tgccaaaccc ttcgcgtttc
      481 gaagatctcg cggcgctcga gacaaacgcc aacgatattt cctccggcgg cggcgatccg
      541 attttcctca acgaactcta cgcgctgaac ctcgcggacc tcaccgcctt cgcgcgcaac
      601 cagggcgtcg cgttcgaagg cgcgccgaac aagaaacagc ttctttcgct cgtcctcgcc
      661 agcgcggcgg agaaaaagcg gccgttgaag gatcgcggct acatcgacgt cacggatcgc
      721 ggcgcgttca tcgtgcacga acacgtgaac taccggctgt atccggaaga tccctacctc
      781 ccggaaagcc ttgtccgtct ctacggtcta aaacgcggtc atcgcgtcga ggtgcaggtc
      841 gaggcgccgc gccccggcga acgctgcccg tccgtcgtgc gcgtcgagtc cgtcatgggc
      901 atgccgcccg ccgacatctc gaaggtcacc cccttcgaag aactcgtccc ctactatccg
      961 ctgcaacgta tcctcctcga atccgaggaa acgcagaaag acgtctcgat gcgcgccgtc
     1021 gacatcctca cgccgatcgg cttcggccag cgcgcgctga tcgtcgcccc gccgcgcacc
     1081 ggcaaaaccg ttctcttgca gaacatcgcc aactcgatca ccgccaacag cccgcacgcg
     1141 aagctcattt tgctgctgat cgacgaacgc cccgag
//
LOCUS       DASPLT010000850         1405 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_153586, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000850 DASPLT010000000
VERSION     DASPLT010000850.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1405)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1405)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1405
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_153586"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..853)
                     /locus_tag="VEA63_08835"
     CDS             complement(<1..853)
                     /locus_tag="VEA63_08835"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HYD84147.1"
                     /translation="MTSFAALQAAARELPREHYAVDRPVDRRGQYQLELAAEFPFAVK
                     RLSFSAQQTPPLTWHTYLEVFVLLSEACRVQMGGATIPLARGDVLVMDHQKLHAVLDF
                     PGAEAEAVVIRFLPDLVRSPGSAAADHLLLLPFYCQMAEQPHVLRASATDAAVVHETL
                     ARLFECCARTEAGLYAQTGSRAYFIVLLHHLARHFRAEERLKDLYARQRAKHGRLREV
                     FEFIAEQYAERISLPAVATLAGLSKPQFHAVFKKAAGMTLVDYVNQVRLTQAARLLQE
                     TERPVVEI"
     gene            complement(986..>1405)
                     /locus_tag="VEA63_08840"
     CDS             complement(986..>1405)
                     /locus_tag="VEA63_08840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HYD84148.1"
                     /translation="LPTTVSAALDFADGSVGSLIASHDVHGYFPRVEVFGTQGSLTLH
                     DANRYTGKVVLRTKDKEETFETPAGFGELGRGLGVAEMAQAIRENRVARASGDLMYHA
                     LETMLAVHDSSAAGRHVRIESKVERPAPFDYASLPQG"
BASE COUNT          265 a          506 c          413 g          221 t
ORIGIN      
        1 cgatctcgac caccggccgc tcggtctcct gcagcagccg cgccgcctgc gtcaaccgca
       61 cctgattgac gtagtcgacg agcgtcatgc ccgccgcttt cttgaacacg gcgtgaaact
      121 gcggcttgct caaccccgcg agcgtcgcca ccgccggcag cgaaatccgt tccgcatact
      181 gctcggcgat gaactcgaat acctcgcgca accgtccgtg tttcgcgcgc tgccgggcat
      241 agaggtcctt caaccgttcc tcggcgcgaa agtgacgcgc gagatggtgc aacagcacga
      301 tgaaatacgc gcgcgacccc gtctgcgcgt agagtcccgc ttccgttcgc gcgcagcatt
      361 cgaacaagcg cgccagcgtt tcgtgcacca ctgccgcgtc cgtcgccgac gcgcgcagca
      421 catgcggttg ttccgccatc tgacagtaga acggcagcaa cagcagatga tccgccgccg
      481 ccgagccggg gcttcgcacc aaatccggca ggaagcggat gacgaccgcc tccgcctccg
      541 cgccgggaaa atccagcacc gcgtgcaact tttgatgatc catcaccagc acgtcaccgc
      601 gcgcgagtgg gatcgtcgct ccgcccatct gcacccggca cgcctccgac agcagcacga
      661 agacttcgag gtaagtgtgc caggtgagcg gcggcgtttg ctgcgcggaa aacgacaacc
      721 gtttcaccgc gaacggaaat tctgccgcga gctcgagttg atactgcccg cgccgatcga
      781 cgggacgatc caccgcatag tgttcgcgcg gcagctcacg ggcggcggct tgaagcgcgg
      841 caaacgaagt catgggacaa tcactcaatc aacttacgcc aagagcccga ggcgtgggaa
      901 gacttcaatc gtcgcgccgc gcagccggcc ggcacgagca ccgcgtcaaa cggcccacag
      961 aaaattccgc ggcgccgcgc atcgttcacc cttgcggcag cgaggcgtag tcgaacggcg
     1021 ccggccgctc gaccttgctc tcgatccgca catgccgtcc ggccgcggac gaatcgtgca
     1081 ccgccagcat cgtctcgagc gcatgataca tcaggtcgcc gctcgcgcgc gcgacccggt
     1141 tttcccgaat cgcctgcgcc atctccgcga caccgaggcc gcggccgagt tcgccgaagc
     1201 ccgcaggcgt ctcgaacgtc tcctccttgt ccttcgtccg caaaacgacc ttgcccgtgt
     1261 agcgattcgc gtcgtgaagc gtgagcgaac cctgcgtgcc gaacacttcg acgcgcggaa
     1321 aataaccgtg cacgtcgtgg ctcgcgatca gcgatcccac gctgccgtcg gcgaaatcga
     1381 gcgccgcgct caccgtcgtc ggcaa
//
LOCUS       DASPLT010000851         1190 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_297309, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000851 DASPLT010000000
VERSION     DASPLT010000851.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1190)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1190)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1190
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_297309"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..551
                     /locus_tag="VEA63_08845"
     CDS             <1..551
                     /locus_tag="VEA63_08845"
                     /inference="COORDINATES: protein motif:HMM:NF025120.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HYD84149.1"
                     /translation="RHHAKAHRHPQRHPGRGTPSPPIRLTAETVDRGRMYPADLHRDA
                     SAIAVVRCIYPRTSISSFGNCDDNRRERFGQVMTRKVTRGSANVFADLSLADAEERQT
                     KLRLAHAINAAIAERSLTQAQAGELLDAAQPKISALANYKLDGFSVERLMTFLTALYR
                     DVQIVIKKKPRSRATGRISVQA"
     gene            complement(588..1100)
                     /locus_tag="VEA63_08850"
     CDS             complement(588..1100)
                     /locus_tag="VEA63_08850"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family glutamic-type intramembrane
                     protease"
                     /protein_id="HYD84150.1"
                     /translation="MRRRGRLELILQRLYSALATWPTPRQWLMVAVEAAWLAPVLIAC
                     GLIGEFVGVSPVLEGGALLRLAVIALFVPALSEELFFRALLLKPNASLWRSALAVAAF
                     VAWHPLQATFFGVDWAAVVLNPVFLIAVAALGVALTRVYLATRSIWPPVALHWIVVLM
                     WKLAGGPSPW"
BASE COUNT          273 a          377 c          361 g          179 t
ORIGIN      
        1 gccgccatca tgcgaaagct catcgtcatc ctcaacgcca tcctggccga gggacgccca
       61 gcccaccaat tcgcttgacc gcggagacgg tagatagagg gagaatgtat ccggcggatt
      121 tgcaccggga cgcctccgcg atcgctgtgg ttcgctgtat ctatccccga actagtatat
      181 cctcctttgg gaattgcgat gacaaccgca gagaacggtt cgggcaagtc atgactcgta
      241 aggtcacccg cggcagcgcg aatgtttttg ctgatctcag cctcgctgat gcggaagagc
      301 ggcaaacaaa attgcgcctg gcgcacgcca tcaatgctgc catcgccgaa aggtcgctga
      361 cgcaagcgca ggccggcgaa ctgctcgacg ctgcacagcc taaaatctcg gcgcttgcca
      421 actacaaact tgatggtttc tcggtggagc gcctcatgac gtttctcacc gcgctttatc
      481 gcgacgtgca gatcgtgatc aagaagaagc cgcgctcgcg ggcgacggga cggatcagcg
      541 tccaggcgta gcgtccgccg atccggcgag cggcaacaaa gctcagatca ccatggcgag
      601 ggaccgcccg ctaacttcca catgagaaca acgatccagt gcagcgcgac gggcggccag
      661 atggagcggg tcgcgagata gacgcgtgtc agcgcgacac cgagcgcggc gaccgcgatc
      721 agaaacacgg gattgagtac gaccgccgcc caatcaacgc cgaaaaacgt agcctgcaac
      781 ggatgccatg cgacaaatgc ggcaacggcg agcgcgctgc gccacagcga tgcattcggc
      841 ttgagcagca atgcgcgaaa gaaaagttcc tcagacaaag cgggcacgaa gagagcgatg
      901 actgcgagac gcagcaaagc gccgccctca agcacgggcg aaacaccgac gaattctccg
      961 ataagaccgc acgcgatgag caccggcgcc agccaagcgg cttcaacggc gaccatcagc
     1021 cattgacgcg gcgttggcca agtcgcgagc gcggagtaga gccgctgcag gatcagctca
     1081 agccgcccgc gccgccgcaa ccacggcgct cttcacttcc gctacggttg cgcgcacgag
     1141 ggcctcgtcg tcgccttcgg ccatgatgcg gacgagaggc tcggtgccgc
//
LOCUS       DASPLT010000852         1616 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_256303, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000852 DASPLT010000000
VERSION     DASPLT010000852.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1616)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1616)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1616
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_256303"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..728
                     /locus_tag="VEA63_08855"
     CDS             <1..728
                     /locus_tag="VEA63_08855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HYD84151.1"
                     /translation="DAWAYRRPVLKKLLEREAPDVIGTQEGVYPQLRDIAADLPDYDW
                     IGLGREGGSKGEFCAIFYRRDRFEPVAFDHFWLSDTPNLVASITWATAYHRMVTWARF
                     RERDTGREFYFWNTHFDHQIETARQKSAQLIRERLAPLDPNVPLILVGDFNCAAGTSR
                     ASEILTNDAGLVDTWTIAAHRTNERLNTFHNYQPPLTEGQRIDWILARPPVDVPTAAI
                     IDHHENGQHPSDHFPVTATLRFP"
     gene            complement(822..>1616)
                     /locus_tag="VEA63_08860"
     CDS             complement(822..>1616)
                     /locus_tag="VEA63_08860"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HYD84152.1"
                     /translation="EVIVYDDGSTDESRSILREYGASIRLIEGEHVAGRPSRECQSNA
                     VFQAFTRSSGEWLFLLDGDDCFVPTKVEQVLQTVNSRSGVSLVQSPVTLIDDHGIEIG
                     RYRDRRFHHPNIRAAIREQNDVDFFYPTSAMVVHRPALEKVLPLDMSVCPELACDTRI
                     GMLMPLLGEVITLDEPLALWRRHSGSYIAELGRSRWFQAQQTMRRVRTFNAHADRYAA
                     PRISLWRNRRFRRQLAGALLPAVVRGRLRKTGTIFLGESKPASARG"
BASE COUNT          327 a          617 c          401 g          271 t
ORIGIN      
        1 ccgacgcctg ggcctatcgt cgacccgttc tgaaaaaact cctcgaacgc gaagcccccg
       61 acgtcatcgg cactcaagaa ggcgtgtatc cgcaacttcg cgacatcgcc gccgacctgc
      121 ctgattacga ttggatcggc ctcggtcgcg aaggcggcag caagggcgag ttctgcgcga
      181 tcttctatcg ccgcgaccgt ttcgaacccg tcgccttcga tcacttctgg ctctccgaca
      241 cgcccaacct cgtcgcgtcg atcacctggg ccaccgcgta tcaccgcatg gtgacctggg
      301 ctcgtttccg cgaacgcgac accggccgcg aattttattt ctggaacacc cacttcgatc
      361 accagattga aaccgcccgc cagaaatccg cgcaactcat ccgcgaacgc ctcgccccgc
      421 tcgatccaaa cgtcccgctg atcctcgtcg gcgatttcaa ctgcgccgcc ggcacgagtc
      481 gcgcctccga aatcctgacc aacgatgccg gcctcgtcga cacatggacc atcgccgcgc
      541 accgcaccaa cgaacgcctc aacacctttc acaactacca accgccgctc accgaagggc
      601 agcgcatcga ctggatcctc gcccgcccgc ccgtcgatgt ccccaccgcc gcgatcatcg
      661 accatcacga aaacggtcag caccccagcg atcacttccc cgtcactgcg acgctccgct
      721 ttccctgatc caactacgcc gtagaaggtg gagcccgacg ttctcgtcgg gctggacagc
      781 atccaccgca tcaacccatc cgacccgacg ccagcactga ctcacccgcg tgccgacgcg
      841 ggtttcgatt cacccaaaaa gattgtgccg gttttccgca agcgaccacg cactaccgct
      901 ggcaacagcg cgcccgcgag ctgacgccga aagcgccggt tgcgccaaag cgaaatccgt
      961 ggcgccgcat agcgatcagc atgcgcgttg aacgtccgca cccgccgcat cgtctgctgc
     1021 gcctgaaacc accgcgatcg ccccagctcc gcgatgtaac tccccgagtg ccgccgccac
     1081 aaagccagcg gctcatcgag cgtgatcact tctcccaaca gcggcatcaa catcccgatg
     1141 cgcgtgtcgc acgcgagctc cggacacacg ctcatgtcga gcggcagcac cttttccagc
     1201 gccgggcgat gcaccaccat cgccgacgtc ggataaaaaa aatccacgtc gttttgctcg
     1261 cggatcgccg cgcgaatatt cggatggtga aatcgccggt cgcgatagcg cccgatctcg
     1321 attccatggt cgtcgataag cgtcacgggc gattgcacca acgacacgcc gctgcggctg
     1381 ttcaccgtct gcagcacctg ctcgaccttc gtcggcacga aacagtcgtc gccatcgagc
     1441 aaaaacagcc actcgccgct cgaccgcgtg aacgcctgaa acaccgcgtt cgactggcac
     1501 tcgcggctcg gccgacccgc gacgtgttcg ccctcgatca accgaatgct cgctccatac
     1561 tcgcgcaaaa tcgaccggct ctcatccgtg ctgccgtcat cgtaaacgat cacctc
//
LOCUS       DASPLT010000853         1007 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_70609, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000853 DASPLT010000000
VERSION     DASPLT010000853.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1007)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1007)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1007
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_70609"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..345
                     /locus_tag="VEA63_08865"
     CDS             <1..345
                     /locus_tag="VEA63_08865"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HYD84153.1"
                     /translation="RIINNGSISAHVPRPNSAPYTAAKHAITGLTKSVALDGRAHDIA
                     CGQIDIGNADTAMSGQMNRALQTAGGNAAEPVFDVKHVADAIVYMAGLPLGVNVPFMT
                     VMATGMRYIGRG"
     gene            417..>1007
                     /locus_tag="VEA63_08870"
     CDS             417..>1007
                     /locus_tag="VEA63_08870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD84154.1"
                     /translation="MPDPALGTVDPDFSSAAPTAREAEATAAAESGQLKVGERWRDLM
                     IEAEIVGSDDSYYASHVGLMERVVVREFPVTPANEWRRGAWERLCALSDSKVVRCIEA
                     HEEGGWRFEVSGIPPTMTMREWMSCRRPSFADIEALMRQLTATLGALHAQGVVHLNIR
                     PEAIFIDETKGEPVYLLGGLEEATLYTQPEVRLAEVD"
BASE COUNT          180 a          261 c          377 g          189 t
ORIGIN      
        1 cggatcatca acaacggctc gatttccgcg cacgtgccgc ggccgaattc ggcgccttat
       61 acggcggcga aacatgcgat cacgggactc acgaaatcgg tcgcgctcga tggtcgcgcg
      121 cacgacatag cgtgtgggca aatcgacatc ggcaacgcgg acaccgcgat gtcgggccag
      181 atgaatcgcg cgctgcagac ggctggcggg aatgcggcgg agccggtgtt cgacgtgaag
      241 cacgtggccg atgcgatcgt ttacatggcg gggctgccgc tgggcgtgaa tgtgcccttc
      301 atgacggtga tggcgacggg aatgcgctac attggacggg gatgatccgc gccgggcttc
      361 cggctaaagt ttcggggtag ggtgacgttt aggagaaagc gcaatttctc tacgccatgc
      421 ccgaccccgc acttgggacg gttgatcctg acttttcatc cgccgctccg actgcccgcg
      481 aagcggaagc gactgctgcg gcggaatcgg gtcaactgaa ggtcggcgag cggtggcggg
      541 atttgatgat cgaggcggag atcgtcggat cggacgattc ttattacgcg agtcatgtcg
      601 gcttgatgga gcgcgtggtg gtgcgcgagt ttccggtgac gccggcgaac gagtggcgtc
      661 gcggcgcgtg ggagcggttg tgcgcgttgt ccgactcgaa ggtggtgcgc tgcatcgagg
      721 cgcacgagga aggtggatgg cgtttcgagg tgtcgggaat tccgccgacg atgacgatgc
      781 gcgaatggat gtcgtgccgg cgtccgagtt ttgcggacat tgaagcgctg atgcggcagc
      841 tgacggcgac gctcggggcg ctgcatgcgc agggcgtggt gcatttgaac atccgcccgg
      901 aggcgatctt catcgacgag acgaagggcg aaccggttta tctgctcggc gggctggagg
      961 aggcgacgct ttatacgcag ccggaagtga ggctggcgga agtcgac
//
LOCUS       DASPLT010000854         1719 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_256338, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000854 DASPLT010000000
VERSION     DASPLT010000854.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1719)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1719)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1719
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_256338"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1104)
                     /gene="lpxK"
                     /locus_tag="VEA63_08875"
     CDS             complement(<1..1104)
                     /gene="lpxK"
                     /locus_tag="VEA63_08875"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HYD84155.1"
                     /translation="MSQSWLKKKVNALELYTVDVILGRRADTGAAIYGAFLQVLSWLF
                     NGIAQARWFLYRNRILHDQPLGCLVVVVGNLTVGGTGKTPVVEKFAKALRDRGRKVAI
                     LSRGYKSKSAPFWKKWWYALTHTAEPPPKIVSDGERVLLDSEEAGDEPFMLARNLPGV
                     IVLVDKNRVKSGAFAIKKFGCDTLVLDDGFQYLPLKGRLNLLLVDKTNPFGNGHLLPR
                     GILREPIKHLKRASYVFLTKSNGERDEELEALIKEHNPDADIIECAHRPRYLQRFGVA
                     PEAPGGREPLTFLKGRRVLAFSGIATPESFEKFLRDLGAKLVARERFLDHYRYADEDF
                     QSLFRIAREQGAECLVTTEKDAVRIPDGYVCPLP"
     gene            1357..>1719
                     /locus_tag="VEA63_08880"
     CDS             1357..>1719
                     /locus_tag="VEA63_08880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007360326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HYD84156.1"
                     /translation="MSYKLFIPGPIAVSEKTLRAMAQPMIGHRSTDFVALYNAIQPEL
                     QALFYTKDPVYLSTSSAWGSMEGAVRNVTKKKVLNCMNGAFSDKWNDVAQRCGFAAGA
                     LKSEWGQPVDPEAVRRELA"
BASE COUNT          338 a          579 c          489 g          313 t
ORIGIN      
        1 cggcagcgga cacacgtaac cgtcgggaat gcgcaccgcg tccttctccg tcgtcaccag
       61 acactccgcg ccctgctcgc gcgcgatccg aaacaacgac tgaaaatcct cgtccgcata
      121 acggtagtgg tcgagaaacc gctcgcgcgc cacgagcttt gcgccgagat cccgcaaaaa
      181 cttctcgaag ctctccggcg tcgcgatgcc gctgaacgcc agcacgcgcc gcccctttaa
      241 aaaagtcagc ggctcgcgcc cgccgggtgc ttccggcgcg acgccgaaac gctgcaaata
      301 acgcggccgg tgggcgcatt cgatgatgtc ggcgtcgggg ttatgctcct tgatgagcgc
      361 ttcaagctcc tcgtcgcgct ctccattcga cttcgtgaga aacacgtagc ttgcgcgctt
      421 cagatgcttg atcggctcgc gcaaaattcc gcgcggcagc agatggccgt tgccgaacgg
      481 attcgttttg tcgaccagca gcaggttcaa ccgccctttc aacgggaggt attgaaaacc
      541 atcgtcgagc accaacgtgt cgcatccgaa ctttttaatc gcgaacgcac ccgacttcac
      601 ccggttcttg tccaccagca cgatcacgcc cggcagattg cgcgccaaca tgaacggctc
      661 gtcgcccgcc tcctccgaat caagcagcac gcgttcgccg tcactcacaa tcttcggcgg
      721 cggctcggcg gtgtgcgtga gcgcatacca ccatttcttc caaaagggcg cggatttgct
      781 tttgtagccg cggctcagga tcgcgacttt gcggccgcgg tcgcgcaagg ccttggcgaa
      841 tttctcgacg acgggcgtct ttcctgtgcc gccgaccgtg agattgccaa cgacgacgac
      901 gaggcaaccg agcggctgat cgtgcaggat ccggttgcga tagagaaacc agcgcgcctg
      961 cgcgatgccg ttgaacagcc acgacaacac ctgcaaaaac gcgccgtaaa tcgccgcgcc
     1021 cgtgtccgcg cggcggccga gaatcacgtc gaccgtgtaa agctcgagcg cgttgacctt
     1081 cttcttgagc cagctttgcg acatgggact agttcaggtt cctcccgctt gcaaagtagc
     1141 ggcaggcctc cgcgcctgcc tgcccgatcg caacaccacg caacgcagac aggcagggac
     1201 gcctgcccca ccactgctcg ccggtcgccg gccggcacgg accttcgccg ctgtatttgg
     1261 aaaatgtgac gcgtgttgcg acatggcttt tcgcgaatga aggttgaccc aacgagcagc
     1321 gtaaactgtt ttccgtagcg cctacgggca cgcaccatga gctacaaact cttcatcccc
     1381 ggccccatcg ccgtctcgga aaaaactttg cgcgcgatgg cgcaacccat gatcggccac
     1441 cgcagcacgg acttcgtcgc gctttacaac gcgatccagc ccgagcttca ggctctcttc
     1501 tacacgaagg atcccgtgta tctttcgacc agcagtgcgt ggggctcgat ggaaggcgcc
     1561 gtgcgcaacg tcacgaagaa gaaggtgctg aactgcatga acggcgcgtt ctccgacaag
     1621 tggaacgacg tcgcgcaacg ctgcggcttc gccgccggcg cgctcaagtc cgaatggggc
     1681 cagcccgtcg atcccgaagc cgttcgccgc gagctcgcg
//
LOCUS       DASPLT010000855         1057 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_50062, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000855 DASPLT010000000
VERSION     DASPLT010000855.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1057)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1057)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1057
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_50062"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1057
                     /locus_tag="VEA63_08885"
     CDS             <1..>1057
                     /locus_tag="VEA63_08885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD84157.1"
                     /translation="RVRQIERRLDDSVYFATLRSEEATTFEEVAPHDFRLKTNGGETL
                     ALTVEFTGEPRRDDGLTFADVRASSVGGWEDYWMKGAAVDFSGSKDPRAAELERRVVL
                     SQYLVRVNYASNFPPSEDGLTNITWFGKHHSEMYYWHAAHFYAWGRTELLEKSLAWYR
                     KILPFAQADATAQGFEGARWPKMSGIDGRASPGSINPFIIWNQPNPIALCELVYRSRP
                     ERATLELYRDVVFESAEFLASFAHLDAAKDRYVLGPPIKNVSEKSGENTTQNPTFELA
                     YWYYGLQVAQQWRERLGLAPEPHWADVLAKLSKLPERDGLYVEIETFPDLFEREGPVP
                     TSMLMVLGFMPKVDIVDT"
BASE COUNT          202 a          277 c          377 g          201 t
ORIGIN      
        1 acgcgttcga caaatcgagc gccggctcga cgacagcgtt tatttcgcga cgttgcgcag
       61 cgaggaagcg acgacgttcg aggaagtggc gccacatgat tttcggctga aaacgaacgg
      121 cggagaaacg ctggcgctga cggtggagtt caccggggaa ccgcggcgcg acgatgggtt
      181 gacgtttgcc gacgtgcgcg cgtcgagcgt gggaggctgg gaggattact ggatgaaagg
      241 cgcggcggtg gacttttccg gcagcaagga tccgcgtgcg gccgagctcg agcggcgggt
      301 ggtgctgtcg caatacctgg tgcgagtgaa ttacgcgagt aattttccgc cgtcggaaga
      361 cggactgacg aacatcacgt ggttcggaaa acatcactcg gaaatgtatt actggcatgc
      421 cgcgcatttt tacgcgtggg gccggacgga gttgctggag aagagcctcg cctggtatcg
      481 caagatcctg ccgttcgcgc aggcggatgc gacggcgcag ggcttcgagg gcgcgcgctg
      541 gccgaagatg tccgggatcg atgggcgggc gtcgccgggc tcgatcaatc catttattat
      601 ctggaatcag ccgaacccga tcgcgctgtg cgaactcgtg tatcgctcgc ggccggagcg
      661 cgcgacgctc gagttgtatc gcgacgtggt cttcgagtcg gcggaatttc tggcgtcgtt
      721 cgcgcatctg gacgcggcga aagatcgcta cgtgctcgga ccgccgatca agaacgtcag
      781 cgagaaatcg ggtgagaaca cgacgcagaa cccgacgttc gagctggcgt attggtatta
      841 tggattgcag gtcgcgcagc agtggcgcga acgactcggg ctcgcgccgg agccgcactg
      901 ggcggatgtg ctcgcgaagt tgtccaagct gccggaacgc gacggcttgt atgtcgagat
      961 cgagacgttt ccggatttgt tcgaacgcga aggccccgtg ccgacctcga tgctgatggt
     1021 gttgggcttc atgccgaagg tggacatcgt ggacacc
//
LOCUS       DASPLT010000856         1586 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_111778, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000856 DASPLT010000000
VERSION     DASPLT010000856.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1586)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1586)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1586
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_111778"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..346
                     /locus_tag="VEA63_08890"
     CDS             <1..346
                     /locus_tag="VEA63_08890"
                     /inference="COORDINATES: protein motif:HMM:NF017534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD84158.1"
                     /translation="LDVFAGDIFALSADQLGTVDAIYDRAALVALPPPMRERYTQMLL
                     TLTRAAPQLLICFEYDQNVMPGPPHAVTAAEVWRHYGAAYRLRLVHVQPVEGGLKGVT
                     PATELVWLLDRQ"
     gene            361..732
                     /locus_tag="VEA63_08895"
     CDS             361..732
                     /locus_tag="VEA63_08895"
                     /inference="COORDINATES: protein motif:HMM:NF021483.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HYD84159.1"
                     /translation="MKTTAAHDARIAKMSFAAVYPMYVAKVEKKGRTVAELHEVITWL
                     TGFSEKKLRELIDERVDFETFFARAKLNPNAKLITGTICGYRIEEIENPLTQKARWLD
                     KLVDELAKGRKMEAILRKPKD"
     gene            943..>1586
                     /locus_tag="VEA63_08900"
     CDS             943..>1586
                     /locus_tag="VEA63_08900"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HYD84160.1"
                     /translation="MLQPVKLVPRRLALAISAATLSAFSLFNTASAAEGDVEEIQVTG
                     SRINRDVGFESPQPVTALEMDELRMFDPGLGVSQQLENLPQFFNNVSSDNIANRVGAD
                     VGQSQLNMRGMGGGRTLVLLDGLRIVPSDRRSSPSVDYLPSTLMQRVDVVTGGASAAY
                     GADALAGVTNFVLNRGFTGLDIRTSAGINEEGDGEFTRGSVTWGDDFMDGRLHV"
BASE COUNT          321 a          484 c          474 g          307 t
ORIGIN      
        1 gctcgacgtg ttcgctggcg acatatttgc gctgagtgca gaccagctcg gcacggtcga
       61 cgccatttac gaccgcgccg cgctcgtcgc gctgccgccg ccgatgcgcg agcgctacac
      121 gcagatgctg ctgacgttga cgcgcgcggc gccgcagctg ttgatctgtt tcgaatacga
      181 ccagaacgtg atgcccggtc cgccgcatgc cgttaccgcc gcggaagtgt ggcggcatta
      241 cggcgctgcg tatcggctga ggctcgttca cgttcagccg gtcgagggtg gcctcaaagg
      301 cgtgacgcct gctaccgaac tcgtctggct tctcgatcga cagtgacgac aggaggcgca
      361 atgaaaacca ccgctgccca cgacgcccgc atcgccaaaa tgagcttcgc ggccgtctac
      421 ccgatgtatg tcgcgaaagt ggaaaagaaa ggtcgcacgg tcgccgagct gcacgaggtg
      481 atcacgtggc tcaccggctt cagtgaaaag aagctgcgcg agttgatcga cgagcgcgtc
      541 gacttcgaaa cgttttttgc gcgcgcgaaa ctgaacccga acgcgaagct gatcaccggc
      601 acgatctgcg gttaccgcat cgaggagatc gaaaatccgc tgacgcagaa agcgcgctgg
      661 ctcgacaagc tcgtcgatga actggcgaaa ggccgcaaga tggaagccat cctgcgcaag
      721 ccgaaggact aatcctggcg cgtcacgtca cgtcgaggcc gagatcgaac gtcgccgcaa
      781 atagtttact cgtccccctt ttgatgtaag cagaaccggc atattctcgc gctcgtcagt
      841 gatgtactgc ttcgcaaact aataagtcac gctcatgtgg tgaccgtacc gtccggtacg
      901 aataattacg caccatctac tcacgtcaaa aaggggaaac gcatgcttca gccagtaaag
      961 cttgtcccgc gccgtctcgc gctcgccatt tccgccgcaa cactttctgc tttctcactg
     1021 ttcaacaccg cgtctgctgc cgaaggtgac gtcgaagaaa tccaggtcac cggttcgcgc
     1081 atcaatcgag acgtgggctt cgaatcgccg caaccggtta ccgcgctcga gatggatgaa
     1141 ctgcgcatgt tcgatcctgg tctcggcgtt tcgcagcagc tcgaaaacct gccgcagttt
     1201 ttcaacaacg tcagctcgga caatattgcg aaccgcgtcg gtgccgacgt gggccagagc
     1261 cagctgaaca tgcgaggcat gggcggcggc cgcacgctcg tgctgctcga tggtttgcgc
     1321 atcgtgccgt ccgatcgccg cagttcaccg agcgtcgatt acctgccaag cacgttgatg
     1381 cagcgcgttg acgtcgtcac cggcggtgct tctgcggcgt acggtgccga tgcactcgcg
     1441 ggtgtgacga acttcgtgct gaatcgcggg ttcaccggcc tcgacattcg cacgagcgcc
     1501 ggcatcaacg aagaaggcga cggtgaattc acgcgcggtt cggtgacgtg gggcgacgac
     1561 ttcatggacg gccggctgca cgtttt
//
LOCUS       DASPLT010000857         1718 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_34197, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000857 DASPLT010000000
VERSION     DASPLT010000857.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1718)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1718)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1718
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_34197"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..840
                     /locus_tag="VEA63_08905"
     CDS             <1..840
                     /locus_tag="VEA63_08905"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HYD84161.1"
                     /translation="PLVRAISFVGSTAVAKHIYETGTKHGKRVQAAGGAKNFLLIMPD
                     AHVAKTVEGVTGAAFGCAGERCMAGSTALVVGDAAKHVLPTLVEATKTLTVGRTDLDA
                     QPAMGPVISAQHRERVLALLAQGEQQGARVLADGRGVRVADAPNGFFLGASIVEQSDP
                     STNALMREEVFGPVLSVMKMDDLGAAIELANRSVYGNGAAIFTRSGQAAREFKHRIKA
                     GMVGINIGVPASLAPFPFSGWGESFFGDLHVQGREGVLFYTQQKVVTTRWFAGGEGDI
                     WRK"
     gene            986..>1718
                     /locus_tag="VEA63_08910"
     CDS             986..>1718
                     /locus_tag="VEA63_08910"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HYD84162.1"
                     /translation="MIPLRLLLVLCCAASAAFAQNVRPLPVERVVVISVDGLRPDLML
                     YADTPVLHGLMKTGTYTMWARTTAVAITLPSHASMLTGVIPRKHRIDWNRDLPLRETV
                     YSHVPTLLELATKAGYTTAIVAGKSKFRALAKPGTVDHVSIPPQDNYTAKDADVAAEA
                     IKLIEAHQPEVMFIHFPSNDSAGHRHGWGSPEQRAATEEADRQIGHVLSALERAGVRE
                     IRVVPMLVSSHSGHFDQIRWLTGDSV"
BASE COUNT          296 a          517 c          585 g          320 t
ORIGIN      
        1 ccgctcgttc gcgcaatctc gtttgtcgga tcgaccgcgg ttgcgaaaca catttacgag
       61 accggcacga agcacggaaa acgtgtgcag gctgcgggcg gcgcgaagaa tttcctgctg
      121 atcatgccgg atgcccacgt ggcgaaaacc gtcgaagggg tgacgggtgc ggcatttggc
      181 tgcgcgggcg agcgttgcat ggcgggctcg acggcgctcg tcgtcggcga tgcggcgaag
      241 cacgtgttgc cgacgctcgt cgaagcgacg aagacgctca cggtcggccg cacggatctc
      301 gatgcgcagc cggcgatggg tccggtgatt tccgcgcagc atcgcgagcg cgtgctcgcg
      361 ctactcgcac agggcgaaca gcagggggcg cgcgtgcttg ccgatggtcg tggcgtgcgg
      421 gtggccgatg cgccgaacgg ctttttcctc ggcgcgagta ttgtcgagca aagcgatccg
      481 tcgacgaacg cgttgatgcg cgaagaggtg ttcggcccgg tgctgagcgt gatgaaaatg
      541 gacgacctcg gcgcggcgat cgagttggcg aatcgctccg tctacggaaa tggtgcggcg
      601 atcttcacgc gttcgggtca agcggcgcgc gagttcaagc atcgcatcaa agcgggcatg
      661 gtcgggatca acatcggcgt gccggcgtcg ctcgctccgt ttcccttcag cggctggggc
      721 gagtcgttct tcggcgatct gcacgtgcag ggccgcgagg gcgtgctttt ttatacgcag
      781 caaaaagtgg tcacgacgcg ctggtttgcc ggcggcgagg gcgacatttg gcggaaatga
      841 agcgcagcaa gaaaagtttg cgggctgaat tttcccgctg ctcacgcagc gagccacatc
      901 ttgtagctct gcgcgtgagc gcagggatca cgcgcggctg ccaccggcct tcttcgaaca
      961 gccatcacct caccttcact ctgccatgat tcctcttcgc ctccttctcg tcctctgttg
     1021 cgccgcatcc gcggcgtttg cgcaaaacgt ccggccgctg ccggtggagc gtgtcgtcgt
     1081 catcagcgtc gatggcttgc ggccggattt gatgctgtat gccgatacac ctgtgctgca
     1141 cggcttgatg aagaccggca cgtatacgat gtgggcgcgc acgacggccg tggcgatcac
     1201 gctgccgtcg cacgcgagca tgctcaccgg cgtcattccg cgaaagcatc gcatcgactg
     1261 gaatcgcgat ttgccgctgc gtgaaacggt ttactcgcac gtgccgacgc tgctcgaact
     1321 cgcgacgaag gcgggttaca cgacggcgat cgtcgccggc aaatcgaagt tccgcgcgct
     1381 cgcgaaaccg ggcaccgtcg atcacgtgtc gattccgccg caggacaact acacggcgaa
     1441 ggacgcggat gtcgcggcag aggcgatcaa gctcatcgag gcgcatcagc ccgaagtgat
     1501 gtttatccat tttccgagca acgattcggc ggggcatcgg cacggctggg gttcccccga
     1561 gcagcgcgcg gcgaccgagg aagcggaccg acagatcggc cacgtgttgt ccgcgctcga
     1621 gcgcgcgggc gtgcgcgaga tccgtgtggt gccgatgctc gtgtcgagcc atagcgggca
     1681 cttcgaccag atccgctggc tcaccggcga ttcggtga
//
LOCUS       DASPLT010000858         1436 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_34202, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000858 DASPLT010000000
VERSION     DASPLT010000858.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1436)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1436)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1436
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_34202"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1154)
                     /locus_tag="VEA63_08915"
     CDS             complement(<1..1154)
                     /locus_tag="VEA63_08915"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007359518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HYD84163.1"
                     /translation="MQSAPVTKYRPFPPVDLPHRQWPNRTLTRAPIWCSVDLRDGNQA
                     LAQPMSVEEKLEYFDLLVKIGFKEIEVGFPSASQIEFDFCRRLIEENRIPDDVAIQIL
                     CQCREDLITRSLEALRGAKQIIFHLYNSTSPAQRRYVFNADRTKIRDIATHAVGFLKE
                     KMAPLVADGTRIRLEYSPESFTSTELEFALEICEAVTDVWQPTVENKIILNLPATVEY
                     ATPNVHADQIEWMCTHLTRRDRTIVSLHTHNDRGTGIAATELALLAGADRVEGTLFGN
                     GERTGNLDIVVVALNLYTHGIHPNLDFSNINEIREVYERCTRLEVPARQPYAGELVFT
                     AFSGSHQDAINKSWAVQKPNEPWDVIYIPIDPADLGRSYKAIIRINSQSG"
BASE COUNT          276 a          342 c          540 g          278 t
ORIGIN      
        1 ttgccggact ggctgttgat gcggatgatc gctttgtagc tgcggccgag atcggcggga
       61 tcgatcggaa tgtagatcac gtcccaaggc tcgttcggct tttgaacggc ccaggatttg
      121 ttgatcgcgt cttggtgcga accgctgaag gcggtgaaga ccagctcgcc ggcgtaaggc
      181 tggcgcgccg ggacttcgag acgcgtgcaa cgttcgtaga cctcgcggat ttcgttgatg
      241 ttcgaaaagt cgaggttcgg gtggatgccg tgggtgtaga gattcagggc gacgacgacg
      301 atatccaggt tgccggtgcg ttcgccattg ccgaagagcg tgccttcgac gcggtcggcg
      361 ccggcgagca gggccaactc ggtggcggcg atgccggtgc cgcggtcgtt gtgcgtgtgc
      421 agggaaacga tcgtgcggtc gcggcgggtc aggtgcgtgc acatccactc gatctggtcg
      481 gcgtggacat tgggcgtggc gtattcgacc gtggcaggga ggttgagaat gatcttgttc
      541 tcaacggtcg gctgccagac gtcggtgacg gcttcgcaaa tctccaaggc gaactcgagt
      601 tcagtgctgg tgaagctttc cggcgagtat tccaggcgga tgcgcgtgcc gtcggcgacg
      661 agcggggcca tcttttcctt gaggaagccg acggcgtgcg tggcgatgtc gcggatcttc
      721 gtgcgatcgg cgttgaagac gtagcggcgc tgcgcgggcg aggtggagtt gtagaggtgg
      781 aagatgatct gcttcgcgcc gcgcagggct tcgagcgagc gggtgatcaa gtcttcgcgg
      841 cattggcaga ggatctggat ggcgacgtcg tcggggatgc ggttttcctc gatcaggcgg
      901 cggcagaaat cgaattcgat ctgggacgcg gaagggaagc cgacctcgat ttccttgaaa
      961 ccgattttca ccagcaggtc gaaatactcg agtttctcct cgacgctcat gggttgggcg
     1021 agggcctgat tgccgtcgcg gagatcgacg ctgcaccaga tcggagcgcg ggtgagcgtg
     1081 cggttgggcc actggcggtg aggcagatcg accggtggaa acggccgata tttcgtaacg
     1141 ggagcggatt gcatgagcga aagttcttct aggttaaaac gcggacaaaa acagacataa
     1201 aatcacgccg gccaacgagg cggcggacaa acccaaggcg aaaacgcacg atcggccgct
     1261 aggcggcgat tcagagatcg tgcgcggacg taagtcgaag cagagcttgg gcgaggattc
     1321 gcatcaagag aggcagagat atcggcgcga aaagggggaa gtcaagaggc ggatggggtg
     1381 gatggggtgg agcgggtggt ggatggctac gggggcgtaa ccggtggaac gagcgc
//
LOCUS       DASPLT010000859         2200 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_256400, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000859 DASPLT010000000
VERSION     DASPLT010000859.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 2200)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2200)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2200
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_256400"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..226
                     /locus_tag="VEA63_08920"
     CDS             <1..226
                     /locus_tag="VEA63_08920"
                     /inference="COORDINATES: protein motif:HMM:NF014663.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="urea amidolyase"
                     /protein_id="HYD84164.1"
                     /translation="PDGQPLILMADAQTIGGYPQVGHVITVDLPLIAQLRPGDGVRFT
                     EIALDEAHKLALAREHALAMLHEGLAQKLG"
     gene            228..983
                     /locus_tag="VEA63_08925"
     CDS             228..983
                     /locus_tag="VEA63_08925"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0017168 - 5-oxoprolinase (ATP-hydrolyzing)
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HYD84165.1"
                     /translation="MARIDLNCDLGEGAGHDRELMPLVTSANIACGAHAGDRETMRAT
                     VGLAREHDVAIGAHPGFRDREHFGRRELALSTEELRRLVRKQIATLAEFAPVRHVKPH
                     GALYNLAARDRAVADAIAEAVSEIDPRLVLFGLTGSELVAAGRARGLRVVGEFFADRT
                     YRSDGSLTPRTQAGALIEDEATAVSQVLRMVTEGVVRATNGADVTIDAETVCLHGDGP
                     HAVAFARQLREALRAAGVEVRPVCHLLDDKRPG"
     gene            complement(1012..>2200)
                     /locus_tag="VEA63_08930"
     CDS             complement(1012..>2200)
                     /locus_tag="VEA63_08930"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HYD84166.1"
                     /translation="LLRTIIDHLPARIYVKDHESRYVLNNVAHLAMLSLESQEQAAGR
                     TTLDFFPNERGRQALADDQKVLTTGHLILNQEKSDFAAGGQLHWSLTTKVPLRDVNQQ
                     VVGLVGISHDITERKLAEQELQHRTHEMETDVLMARQVQETFLPRTFPVFPRGVPLES
                     SALRFAHRYIPATTLGGDFYSIVQLSDTKCGVLVCDVMGHGVRAGLLTALIRGVVGEM
                     GERAENPANVLSEINRSLAPILEQTGQPVFATAFFGVIDTGSNTLLYGNAGHPTTLIR
                     RADGTIARLTSPDPEPAAGLIAGFAYARHECAFEAGDLLLGYTDGVIEAPDASGQLYG
                     EERLIALLENSRALSGEEVCERLVQELAAFSGKTVFEDDVCFVTIESTGTTCLVQPVT
                     YDI"
BASE COUNT          380 a          605 c          817 g          398 t
ORIGIN      
        1 gccggacggg cagccgctca tcctgatggc ggacgctcaa acgatcggtg ggtatccgca
       61 agttggacac gtcatcaccg tcgatctgcc gctgatcgcg caattgcggc cgggcgacgg
      121 cgtgcggttt accgaaatcg ctttggacga ggcgcacaag ctggcgctcg cgcgcgagca
      181 tgcgttggcg atgctgcacg aaggcctcgc gcaaaaactc ggctgacgtg gcaaggatcg
      241 atctcaactg cgatctcggc gaaggtgccg gacacgaccg ggagctgatg ccgctcgtga
      301 cgtcggcgaa catcgcatgc ggcgcgcacg cgggcgaccg cgagacgatg cgtgcgacgg
      361 tcgggctcgc gcgcgaacac gatgtagcga tcggcgcgca tcctgggttc cgggatcgcg
      421 agcattttgg gcgcagggag ctggcgcttt cgacggagga gttgagacgc ttggtgcgca
      481 agcagatcgc gacgctcgca gagttcgcgc cggtgcgaca cgtgaagccg catggcgcgc
      541 tctacaattt agcggcgcgg gatcgtgcgg ttgccgacgc gatcgcggaa gcggtgagtg
      601 agatcgatcc gaggctcgtg ctgttcgggc tcacggggag cgagttggtc gcagcgggcc
      661 gcgcgcgagg attgcgcgtg gtcggagaat tttttgcgga tcgcacgtat cgctcggatg
      721 ggtccttgac gccgagaacg caggccgggg cgctcatcga ggatgaagcg acggccgtct
      781 cgcaggtgtt gcgcatggtg accgaaggcg tggtgcgggc gacgaacggc gcggacgtga
      841 cgatcgacgc cgagacggtt tgcctgcacg gcgacgggcc gcacgcggtg gcgtttgcgc
      901 gccaattacg cgaagcgttg agagcagccg gtgtcgaggt gcggccggtt tgtcatctat
      961 tagatgacaa acgtccgggc tgagcgcgtt ggggtaacgc cccttccgcg gtcaaatatc
     1021 gtaggtcacc ggttgcacga ggcaggttgt gccggtggat tcgatggtga cgaagcagac
     1081 gtcatcctcg aaaacggttt tgccgctgaa cgccgcgagc tcttgaacga ggcgttcgca
     1141 gacttcttcg ccgctgagtg cgcgcgaatt ttcgagcagc gcgatgagcc tttcctcgcc
     1201 gtaaagctgg ccggaagcgt ccggcgcttc gatgacgccg tcggtgtagc cgagcaagag
     1261 atcgccggct tcgaaggcgc attcgtgacg cgcgtaggcg aaacccgcga tcaggccggc
     1321 ggcgggttcg ggatcgggag acgtgagtcg cgcgatggtg ccgtcggcgc ggcggatcaa
     1381 agtcgtcggg tgcccggcgt tgccgtagag gagggtgttc gatcccgtgt cgatcacgcc
     1441 gaagaacgcg gtggcgaaca ccggctggcc ggtttgctcg aggatgggag cgaggctgcg
     1501 attgatttcg ctgagaacgt tggcggggtt ttcggcgcgt tcgcccattt cgccgacgac
     1561 cccgcgaatg agggcggtga gcaaaccggc gcgaacgccg tggcccatca cgtcgcacac
     1621 caggacgccg catttcgtgt cggagagttg aacgatgcta taaaaatcgc cgccgagcgt
     1681 cgtcgcggga atgtagcgat gcgcgaaacg aagcgcactg gactcgagcg gcacgcctcg
     1741 cgggaaaacg ggaaacgtgc gcggaagaaa cgtctcctgc acctggcgcg ccatgagcac
     1801 gtcggtttcc atctcgtgcg tgcggtgctg cagttcctgc tcggcgagtt tgcgctccgt
     1861 aatgtcgtgg ctgataccga cgagaccgac gacctgttgg ttgacgtcgc ggagcggaac
     1921 cttggtggtg agcgaccagt ggagctggcc gccggcggcg aaatcggatt tctcctggtt
     1981 gagaatgagg tggccggtgg tgaggacctt ttgatcgtcg gcgagcgcct gccggccgcg
     2041 ttcgttggga aaaaaatcga gcgtggtgcg gcccgcggcc tgctcctggc tttcgaggct
     2101 gagcatcgcg aggtgcgcga cgttgttgag gacgtaacgc gattcgtggt ctttgacgta
     2161 gatgcgcgcg ggcaggtggt cgatgatcgt gcggagaagg
//
LOCUS       DASPLT010000860         1088 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_111847, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000860 DASPLT010000000
VERSION     DASPLT010000860.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1088)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1088)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1088
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_111847"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..627)
                     /locus_tag="VEA63_08935"
     CDS             complement(<1..627)
                     /locus_tag="VEA63_08935"
                     /inference="COORDINATES: protein motif:HMM:NF028174.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase dimerization domain-containing
                     protein"
                     /protein_id="HYD84167.1"
                     /translation="MNTSDANFPVDSSGSSAASAPSPEHILKTGTAFWTSKALLSAVE
                     LGVFTQLAQRPGTLEDVRARLGLHPRSARDFLDTLVALGFLERNEGVYANTRDTDMFL
                     DKQKSSYIGGLLEMANARLYGFWSGLTEGLKTGEPQNEAKRGGAKFFEELYANEDRLR
                     QFLKAMTGVSRGANLAIARGFPWEKYRSFVDAGTAQGDLAAQVALAQPH"
     gene            complement(798..>1088)
                     /locus_tag="VEA63_08940"
     CDS             complement(798..>1088)
                     /locus_tag="VEA63_08940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012377404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HYD84168.1"
                     /translation="GVRNRDFTATMRQEALGIHAALTRVKEMVAAGVKNPDRLIAEAT
                     HILSQRRRLRIIYIAIVDATTMETMREVVPGQSMLAIAAWVDEIRLIDNMVL"
BASE COUNT          230 a          412 c          242 g          204 t
ORIGIN      
        1 atgcggctgc gcgagcgcga cctgcgccgc gagatcgccc tgcgccgtcc ccgcatccac
       61 aaacgaccga tacttttccc acggaaaccc gcgcgcgatt gccagattcg ccccgcgact
      121 cactcccgtc atcgccttca agaactgccg caaccgatcc tcattcgcat acagctcctc
      181 gaaaaacttc gcgcccccac gcttcgcctc gttctgcggc tcgcctgttt tcagcccttc
      241 cgtcagcccg ctccaaaaac catacagccg cgcattcgcc atctccagca gcccgccgat
      301 atacgacgac ttctgcttgt ccaaaaacat atccgtgtcg cgcgtgttcg catagacgcc
      361 ctcgttccgc tcgagaaatc cgagcgcgac cagcgtatcc aaaaaatccc tcgccgaccg
      421 cggatgaagc ccgagccgcg cgcgaacatc ctccaacgtt cccggccgct gcgcgagttg
      481 cgtgaacacc ccgagttcga ccgcactcag caatgccttt gacgtccaga acgccgttcc
      541 cgtttttaaa atgtgttcag gcgaaggcgc cgaagcggcc gacgaaccag acgaatcaac
      601 cgggaaattc gcatcggagg tgttcatagg aggggccaaa caaagatcga tcgagcacct
      661 caattgtcaa cggccccggg ccccgcctca cgcccatctt acctcccgct gatcatccca
      721 tcacgccttc tccgatacga acatccacca tgcagaatcc aaacgagccc ctctcgcaaa
      781 gttccgcgct catcacttca caacaccata ttgtcaatca acctgatctc gtccacccat
      841 gccgcgatcg ccagcatgct ctgtcccgga acgacctcgc gcatggtctc catcgtcgtc
      901 gcatccacga tcgcgatata gataatccgc agccgccgcc gctgactcag gatgtgcgtc
      961 gcttccgcga tcaatcgatc aggattcttt acgcccgccg ccaccatttc cttcacgcgc
     1021 gtaagtgcgg catggatacc gagcgcctcc tgccgcatcg tcgccgtaaa atcccgattc
     1081 cgcacgcc
//
LOCUS       DASPLT010000861         1379 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_275956, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000861 DASPLT010000000
VERSION     DASPLT010000861.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1379)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1379)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1379
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_275956"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..714
                     /locus_tag="VEA63_08945"
     CDS             <1..714
                     /locus_tag="VEA63_08945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HYD84169.1"
                     /translation="AAVHSTAWKRMFDEYLRRRERERGEVFREFSHAGDYRSYVDGRP
                     RYEGVKAFLASRGLHLPFGETSDPPNAETICGLGNRKNELFNAIVESEGVRVYRSSIA
                     LIHSLQDAGVKVGLATSSRNAALVLAKTGAASLFATVVDGLVSERVGLAGKPAPDIFL
                     TACAQLGANPWRAIVIEDAVSGVRAGVAGGFALVVGVARENNVEELRENGADAVVGDL
                     AELDVAALDRKVRLKAGGA"
     gene            732..>1379
                     /locus_tag="VEA63_08950"
     CDS             732..>1379
                     /locus_tag="VEA63_08950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HYD84170.1"
                     /translation="MTTLKKPRLNFWQLWNMSFGYVGIQFGFALQNANVSRIFETLGA
                     SVDEIPILWIAGPVTGLVVQPIVGYISDKTWNRLGRRKPYFLVGAILASLALLVMPNS
                     PALWFAAGMLWILDASINITMEPMRAFVGDMLPDEQRTTGFAVQTFFIGASSVIGSLL
                     PYLLTNVFDVANTAPEGQLPASVKWSFYLGGGVYFLAVMWTILRTKEYSPEQQAAF"
BASE COUNT          241 a          388 c          464 g          286 t
ORIGIN      
        1 gcggcggtgc attcgacggc gtggaaacgg atgttcgatg aatatctccg gcgtcgggag
       61 cgtgaacgcg gcgaagtgtt tcgcgaattt tcgcatgcgg gcgattaccg cagctatgtc
      121 gatggccggc cgcgctacga aggcgtgaaa gcatttctcg catcgcgtgg gcttcatctg
      181 cctttcggcg aaacgagtga tccgccgaac gcggaaacga tctgcggact cggcaatcgg
      241 aaaaacgaac tgttcaacgc gatcgtcgaa agcgaaggcg tgcgcgttta tcgctcctcc
      301 atcgcgctga ttcattcgct gcaggacgca ggcgttaaag tggggctcgc cacgtcgagc
      361 cggaatgcgg cgctcgtgct cgcgaagacc ggcgcggcgt cgctgtttgc gacggtggtc
      421 gacgggcttg tatccgaacg cgtgggactc gcgggcaagc cggcgccgga tatttttctc
      481 acggcgtgcg cgcagctcgg cgcgaatcct tggcgtgcga tcgtgatcga ggatgcggtt
      541 tcgggcgtgc gcgccggggt ggcgggcggg tttgcgttgg tcgtgggcgt tgcacgggaa
      601 aacaacgtcg aggaattacg ggagaatggc gccgacgcag tcgtgggcga tttggccgag
      661 ttggatgtcg cagcgctcga tcggaaggtt cgcctcaaag ccggcggagc gtgagactcc
      721 gcgccttccc catgactacc ctgaaaaaac cccgcctcaa cttctggcag ctctggaaca
      781 tgagctttgg ctatgtcggc attcagttcg gtttcgcgct tcaaaacgca aacgtcagcc
      841 ggatcttcga aacgttggga gcgagcgtgg acgagattcc gattctctgg atcgcgggtc
      901 cggtgaccgg cttggtcgtg cagccgatcg tcggctacat aagcgacaag acgtggaacc
      961 ggctcggtcg gcgcaaacct tacttcctcg tcggagcgat cctcgcttcg ctggcgcttt
     1021 tggtgatgcc gaactcgccc gcgctgtggt tcgccgcggg catgctgtgg atcctcgatg
     1081 cgtcgatcaa catcacgatg gagccgatgc gcgcattcgt cggcgacatg ctgccggacg
     1141 aacagcgcac caccggtttc gcggtgcaga cgtttttcat cggcgcgagt tcggtgatcg
     1201 gttcgctgct gccgtatctg ctcacgaacg tcttcgacgt cgccaacacc gcgccggagg
     1261 gacaactgcc ggcgtcagtg aagtggtcgt tctatctcgg cggcggcgtg tatttcctcg
     1321 cggtgatgtg gacgatcctg cggacgaaag aatattcgcc ggagcagcaa gccgcgttt
//
LOCUS       DASPLT010000862         1493 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_153792, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000862 DASPLT010000000
VERSION     DASPLT010000862.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1493)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1493)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1493
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_153792"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..301)
                     /locus_tag="VEA63_08955"
     CDS             complement(<1..301)
                     /locus_tag="VEA63_08955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-hydroxyacyl-ACP dehydratase"
                     /protein_id="HYD84171.1"
                     /translation="MSEVTDFIPHRPPFLFVDDLVSETEDSLIARRTFRADEDFYKGH
                     YPGAPITPGVILCEAVFQTGALYMSRRARAAGAQPGEGVPLLAKITDVRFRNPVFP"
     gene            complement(298..>1493)
                     /locus_tag="VEA63_08960"
     CDS             complement(298..>1493)
                     /locus_tag="VEA63_08960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HYD84172.1"
                     /translation="KDIRFVNPQMDVWTTFTREDYTRVLVEQFGLVREKVESFYDYLR
                     AMNYYDNNPETTGEMLERFFPGRDDVKRLLMEPIAYANGSTLDDPAITYGIVFSNFMG
                     SGVYTFRGGSDLLIEKMIAELKRNGVEVRKKVLVGKILVEERNGQKVACGIVASSGRV
                     IRAKAVLSNANIHNTIFRMAGAENFPADYVAEAKAVRINSSSCQVYLGIRKGESIPHI
                     GDLVFTSANPKFSSAELTDFRTTSRTFSVYYPDTRPGSDRYTVVVSLNGRYGDWNALS
                     DEDYEREKERLIEESLVALEKFIPDVRAKIDWKEAATPRTIERYTTHANGTSFGTKFE
                     GLKVSMELPEKLPGLFHAGSVGIIMSGWLGTINYGVIVANKIDKLLFDAKRANQASGA
                     AAAIS"
BASE COUNT          297 a          506 c          387 g          303 t
ORIGIN      
        1 ccggaaaaac cggattgcga aaccgcacgt cggtgatctt cgccagcaac ggcacgccct
       61 cgccgggttg cgctcccgcc gcacgcgcac gacgcgacat gtagagggcg cccgtttgga
      121 aaaccgcctc gcacaagatc acacccggcg tgatcggcgc ccctggataa tgccctttgt
      181 agaaatcctc gtccgcgcga aacgtccgcc gcgcgatcag ggagtcctcg gtttcgctga
      241 ccaaatcgtc gacgaaaaga aacggcggcc ggtggggaat gaaatcggtg acttcgctca
      301 tgaaattgcc gcagccgcac cgctcgcctg gttcgcgcgc ttcgcatcga acagcagttt
      361 gtcgatcttg ttcgccacga tgacgccgta gttgatggtg ccgagccagc ccgacatgat
      421 gatgccaacc gaaccggcgt gaaacagccc cggaagtttt tccggcagct ccatcgagac
      481 tttcaatccc tcaaacttcg tgccgaacga cgtgccgttc gcatgcgtcg tgtagcgttc
      541 gatcgtccgt ggcgtcgccg cttccttcca gtcgatcttc gcgcggacgt cagggatgaa
      601 cttctccaat gccaccagcg attcctcgat cagccgctct ttttcccgct cgtaatcctc
      661 gtccgacaac gcattccaat caccgtatcg gccgttcaac gacacgacca ccgtgtagcg
      721 atccgatccc ggacgcgtat ccggataata aacggaaaac gtccggctcg tggtgcggaa
      781 atcagtcagc tccgcgctgc tgaacttcgg attcgccgac gtgaacacga ggtcgccgat
      841 gtgcggaatg ctctctcctt tgcggatgcc gagatacacc tggcacgagc tcgagttgat
      901 ccgcaccgcc ttcgcctccg caacgtagtc cgccggaaaa ttctccgcgc ccgccatccg
      961 gaaaatcgtg ttgtggatgt tcgcattcga cagcaccgcc tttgcgcgaa tcacccgccc
     1021 gctgctcgcg acaattccgc acgccacttt ctgcccgttg cgttcctcca cgagaatctt
     1081 gcccacgagc acctttttcc gaacctccac gccgttgcgc ttcagctccg cgatcatctt
     1141 ctcgatcagc agatcggagc cgccgcgaaa cgtgtagacg cccgacccca tgaagttcga
     1201 aaacacgatg ccatacgtga tcgccgggtc gtcgagcgtc gaaccgttcg cataggcgat
     1261 cggctccatc aacaaccgct tcacgtcgtc ccgccctggg aaaaaccgct cgagcatctc
     1321 gcccgtcgtc tccgggttgt tgtcgtaata gttcatcgcc cgcaggtagt cgtagaacga
     1381 ttccaccttt tcgcgcacga ggccgaactg ctcaacgagc acccgcgtgt aatcctcgcg
     1441 cgtgaaagtc gtccatacat ccatctgcgg attcacgaac cggatgtcct tca
//
LOCUS       DASPLT010000863         1681 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_275984, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000863 DASPLT010000000
VERSION     DASPLT010000863.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1681)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1681)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1681
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_275984"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..436
                     /locus_tag="VEA63_08965"
     CDS             <1..436
                     /locus_tag="VEA63_08965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD84173.1"
                     /translation="RLVLAEPAATRIGRAFYRAFHHEPCDPETLRAPWVFGAEAKSGE
                     FANMGMAWALFVRDRANVAAPLAEYGLAIAEVSFRDGLAYPATGGLSKPALLPAPAIR
                     GLLRIEQRLPQRFWRFWGLRMIVVLEKTGTANVMSNQRPAVE"
     gene            433..>1681
                     /locus_tag="VEA63_08970"
     CDS             433..>1681
                     /locus_tag="VEA63_08970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009513511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional class I SAM-dependent
                     methyltransferase/glycosyltransferase family 2 protein"
                     /protein_id="HYD84174.1"
                     /translation="MSIERNDSLVRHFDWVAEHDAAARRRQRGFHCQLAEFYRRHIPE
                     GSRVLEWGCGAGGLLNALKPSRGLGLDFSERMIAKARTEAAPNCEYRLGDVLHTDVDE
                     SFDHIVLSYLSGYLPDIQRAFERLHATAHARTRLHLTSLNTLWSFPLALAGRLGVVMP
                     QPSSNWLSRMDLINLLELAGWEVVNSSTEQLMPVRVPLVAGFFNRFLVRLPFFRHFGI
                     TLSITARPKRRPLIEGEISCSVIVPARNEAGNIRAALERIPVLGRRTEVIFVEGNSRD
                     DTWETIQREMAAYRGPHLVRALQQPGKGKWDAVREGFAAARGEVLVIQDADLTAPPED
                     LPKFYEAVASGAAEFANGSRLVYPMEKQAMRFLNLVGNKFFALALSYVVGQPLKDSLC
                     GTKMLLRSDYERLVRRIAPFGDFD"
BASE COUNT          263 a          447 c          630 g          341 t
ORIGIN      
        1 ccggctggtg ctggcggagc cggcggcgac gcgtattggg cgcgcgttct atcgcgcgtt
       61 tcaccacgag ccgtgcgatc cggaaacgct gcgcgcgccg tgggtgttcg gcgcggaggc
      121 gaagagcggt gagtttgcga acatgggcat ggcgtgggcg ctgtttgtgc gcgaccgagc
      181 gaacgtggcg gcgccgctcg cggagtatgg gctggcgatc gcagaggtgt cgtttcgcga
      241 cggtctcgct tatccggcga cgggcggatt gtcgaagccg gcgctgctgc cggcgccggc
      301 gattcgcggc ctgttgcgga tcgaacagcg gttgccgcag cggttttggc gcttctgggg
      361 attgcgcatg atcgtggttc tcgagaaaac cgggactgcc aacgttatgt cgaaccaacg
      421 tcccgccgtc gaatgagcat cgagcgaaac gattcgctcg tccggcattt cgactgggtg
      481 gccgaacacg atgcagcggc gcgccggcgg cagcggggct ttcactgtca gcttgcggag
      541 ttttaccggc ggcatattcc ggagggaagt cgcgtgctcg agtggggttg cggcgcgggc
      601 ggcttgttga acgcattgaa accgtcgcgt ggactcgggc tcgatttcag cgagcgcatg
      661 atcgcgaagg cgcgcacgga ggcggcgccg aattgcgagt atcgcctcgg cgatgtgttg
      721 cacacggacg tggacgagtc gtttgaccac atcgtgctga gttatctgag cggttatctg
      781 ccggacatcc agcgggcgtt cgagcggttg catgcgacgg cgcacgcgcg cacgcggctg
      841 catctgacgt cgttgaacac gctgtggagt tttccgctgg cgctcgccgg ccggctcggc
      901 gtggtgatgc cgcagccgtc gagcaactgg ttgtcgcgga tggatctgat caacctgctc
      961 gagctcgcgg gttgggaagt ggtgaacagc tcgacggagc agttgatgcc ggtgcgcgtg
     1021 ccgctggtgg cgggtttttt taatcggttt ctcgtccggc tgccgttctt ccgtcacttc
     1081 ggtatcacgc tttccattac agcgcggccg aagcggcgtc cgctcatcga aggtgaaatc
     1141 tcctgctccg tgatcgtgcc ggcgcgcaac gaggcgggga acattcgcgc ggcgctcgag
     1201 cgaattccgg tgctcgggcg gaggacggag gtgattttcg tcgaaggcaa cagtcgcgac
     1261 gacacgtggg agacgattca gcgcgagatg gcggcttatc ggggcccgca tctcgtgcgc
     1321 gcgctccagc agccggggaa ggggaaatgg gatgccgtgc gcgagggatt cgcggcggcg
     1381 cgcggcgagg tgttggtgat ccaggatgcg gatctcaccg cgccgccgga ggatctgccg
     1441 aagttttacg aagcggtcgc gagtggggcg gcggagttcg cgaacgggag tcgtctggtg
     1501 tatccgatgg aaaagcaggc gatgcgtttc ctgaatttgg tggggaataa attcttcgcg
     1561 ctggcgttga gctatgtggt cgggcaaccg ctgaaggaca gtttgtgcgg gacgaagatg
     1621 ttgttgcggt ccgattatga acgattggtg cgacggatcg cgccattcgg agacttcgat
     1681 c
//
LOCUS       DASPLT010000864         2356 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_11030, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000864 DASPLT010000000
VERSION     DASPLT010000864.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 2356)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2356)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2356
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_11030"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            42..1469
                     /gene="ahcY"
                     /locus_tag="VEA63_08975"
     CDS             42..1469
                     /gene="ahcY"
                     /locus_tag="VEA63_08975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HYD84175.1"
                     /translation="MPAATKDATAPDYLVKDITLAEWGRKEISIAEHEMPGLMAVRKK
                     FGAAKPLAGVRITGSLHMTIQTAVLIETLTELGADVRWASCNIFSTQDHAAAAIAKSG
                     VPVFAWKGETLEEYWELTLKAVTFPGGKGPQLVVDDGGDVTLLIHKGCELEEGSGWVN
                     TPSGSHEEQVIKNLLKKVHAQDPLRWTTIAKEWRGVSEETTTGVHRLYQMLEKGKLRV
                     PAINVNDSVTKSKFDNLYGCRESLADGLKRATDVMIAGKVACVCGYGDVGKGSAHSLK
                     GFGARVIVTEIDPINALQAAMEGFEVNTIESTLGVADIYVTTTGNKDIITLEHMRKMK
                     DQAIVCNIGHFDNEIQVDRLNTSDAKRINVKPQYDLYTFPGGPSIYLLAEGRLVNLGC
                     ATGHPSFVMSNSFTNQTLAQIDLWKNKDTYAIGVYRLPKHLDEEVARLHLEKIGAKLT
                     TLTPDQASYLGVPVEGPYKPDHYRY"
     gene            complement(1632..1725)
                     /locus_tag="VEA63_08980"
     tRNA            complement(1632..1725)
                     /locus_tag="VEA63_08980"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1689..1691),aa:Ser,seq:gct)
     gene            complement(1804..>2356)
                     /locus_tag="VEA63_08985"
     CDS             complement(1804..>2356)
                     /locus_tag="VEA63_08985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD84176.1"
                     /translation="FYEACKDSADRIDDVAELMNWGGSDSDEDDDDEDEDDEDDSSDF
                     AQGSDVYTLHKNPIFVSTKALYLGLRRHWELGAGDSTKVPQPLALAMLSSLHRGEEQA
                     VQAIHALDFGDYAMAVSLFKRALSALNGTLALLNDQAAVSHRTVAAYRETALPRLFDL
                     REIWLRVVHECREELERPTDGDS"
BASE COUNT          503 a          708 c          719 g          426 t
ORIGIN      
        1 ggccgagcgc cgtcaatcgt aacatccaaa ttctgaatac aatgcccgct gccaccaaag
       61 acgccacggc tcccgattat ctcgtcaaag acatcacgct cgccgagtgg gggcgcaaag
      121 agatctccat cgccgaacac gaaatgccgg gcctcatggc cgtccgcaaa aagttcggcg
      181 cggccaagcc gctcgcgggc gtgcgcatca cgggctcgtt gcacatgacg attcagacgg
      241 cggtgctgat cgagacgttg accgaactcg gcgccgacgt gcgctgggcg tcgtgtaata
      301 ttttctccac gcaggatcac gcggcggccg cgatcgcgaa gtccggcgtg ccggtcttcg
      361 cttggaaggg cgagacgctc gaagaatatt gggagctgac cttgaaggcg gtgacgtttc
      421 ccggcggcaa gggcccgcaa ctcgtcgtcg acgacggcgg cgatgtcacg ctgctgattc
      481 acaaaggctg cgaactcgag gaaggtagcg gttgggtgaa tacgccttcc ggctcacacg
      541 aggagcaggt gatcaagaac ctcctcaaga aagttcacgc gcaggatccg ctgcgctgga
      601 cgacgatcgc gaaggagtgg cgcggcgtgt ccgaggagac gacgacgggc gtgcatcgcc
      661 tctatcagat gctcgagaag ggcaaactcc gcgtgcccgc gatcaacgtc aacgactcgg
      721 ttacgaagtc gaaattcgat aatctctacg gctgccgcga gtcgctcgcg gacggtctca
      781 agcgcgccac cgacgtcatg attgctggca aggtcgcttg cgtgtgcggc tacggcgacg
      841 tcggcaaagg ctcggcgcat tcgctaaagg gattcggcgc tcgcgtgatc gtcacggaga
      901 tcgatccgat caatgcgctc caggcggcga tggaaggttt cgaggtcaac acgatcgaga
      961 gcacgctcgg ggttgctgac atctacgtga cgacgaccgg caacaaggac atcatcacgc
     1021 tcgagcacat gcggaagatg aaggaccagg cgatcgtctg taacattggt cacttcgata
     1081 acgagattca ggtcgaccgg ttgaatacat cggacgcgaa gcgcatcaac gtgaagccgc
     1141 agtatgacct ctacacgttc cccggcggcc cgagcattta cctgctcgcg gaaggccggc
     1201 tcgtgaacct cggctgcgcg acgggccatc cctcgttcgt gatgtcgaac agcttcacga
     1261 atcagacgct cgcgcaaatt gatctctgga agaacaagga cacttacgcg atcggcgtgt
     1321 atcgcctgcc gaagcacctc gacgaggaag tcgcgcggct gcacttggaa aagatcggcg
     1381 cgaagctgac gaccctcacg cctgatcagg cgtcgtatct cggcgtgccg gtcgaaggcc
     1441 cctacaagcc ggaccactac cgctattgag cggaggcgct caacgcgctg ttcgcttgga
     1501 cggttttcaa acccgcgctg atcggcgcgg gtttttttgc ggctattcga tcgccgacgg
     1561 actgaaggtg gttcggacgg ccaagtctta cggcacttgg cgtgggccag aaatccgaca
     1621 aaaaaaagac ctggcggaga gggagggatt cgaaccctcg gtagggattt aaacccctac
     1681 aacgctttag caaagcgccg ctatcgacca ctcagccacc tctccaaaac aggttttgcg
     1741 aggcaaacgc agcctcatga aagtgcaagg cggatttctc cgcaaaacgc gatcaatcgc
     1801 cgctcagcta tcgccgtcgg tggggcgctc gagttcctcg cggcactcgt gcaccacgcg
     1861 cagccaaatt tcgcggagat cgaagagccg cggcagcgcg gtctcgcgat acgccgccac
     1921 cgtgcgatgc gacaccgccg cctgatcgtt taacaaagcg agcgtgccgt tcaacgcgct
     1981 cagggcgcgc ttgaagaggc tgaccgccat cgcgtagtcg ccaaaatcca acgcgtgaat
     2041 ggcctgcacg gcctgttctt cgccgcggtg cagcgaggag agcatcgcga gcgcgagcgg
     2101 ctgcggcact ttcgtgctgt cgccggcgcc gagttcccaa tgacgccgca aaccgagata
     2161 aagcgccttg gtggagacga agatcggatt tttgtgcagc gtgtagacat cgctgccctg
     2221 cgcgaagtcg gaggaatcgt cctcgtcgtc ttcatcctca tcatcgtcat cctcatcgga
     2281 atcgctgccg ccccaattca tgagttcggc gacgtcgtcg atgcgatcgg cgctgtcttt
     2341 gcacgcctcg taaaat
//
LOCUS       DASPLT010000865         2297 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_174858, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000865 DASPLT010000000
VERSION     DASPLT010000865.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 2297)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2297)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2297
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_174858"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..361)
                     /locus_tag="VEA63_08990"
     CDS             complement(<1..361)
                     /locus_tag="VEA63_08990"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HYD84177.1"
                     /translation="MNTSAPVRVAVIGAGLMGREVASAFGRWFALTDCPVRPELVGVC
                     DVNAAALDWFRQVPTVRHFDTDHRVLLARPDIEVVYVAVPHHLHQAIYLDVLRAGKDL
                     LAEKPFGVDLASARRIRD"
     gene            complement(358..1188)
                     /locus_tag="VEA63_08995"
     CDS             complement(358..1188)
                     /locus_tag="VEA63_08995"
                     /inference="COORDINATES: protein motif:HMM:NF013916.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD84178.1"
                     /translation="MKSARLNRLFNPATRRCFDVAIDHGFFNEASFLSGIENLPRVVE
                     ALVAAAPDAIQLTVGQAPHLQALPVRGKPALVLRTDVANVYGRELPRTLFSRIIEQPV
                     LQAVRLDATCVVVNLFAIPGQSEVAEHCVQNILHLKPECDHYGMPLMIEPLVFQPNAK
                     AGGYMVDGDPQKIIPLVRQAVELGADIIKADPTDDISVYHKVVEIAGGIPVLVRGGSK
                     ATEEEILARTQALIEQGASGIVYGRNIIQHSKPAAITRALMAVVHDGVSAREALKLLA
                     "
     gene            1469..>2297
                     /locus_tag="VEA63_09000"
     CDS             1469..>2297
                     /locus_tag="VEA63_09000"
                     /inference="COORDINATES: protein motif:HMM:NF014116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate binding domain-containing protein"
                     /protein_id="HYD84179.1"
                     /translation="MGSPSRLLATGYLAFAWIASAPAASDLVLNGSFEQTVHDFPAGW
                     SRLDSAVAGATATSVIDVGVQGEKSVRIDRTGATVSAGSAGPGIAQHDLALKPGERYM
                     LTFQARGSPASSSALRVWIQIPGASEFPPLLAESVPLTTDWTRHEFGFEFPEAAPAGR
                     ATLRFVVNSPATVWLDNIALSGAAAAKADISRPQPRLPASSSRNLVPNASFEAGADGW
                     LSLGKPLGFGGNVAGLYGDIVGDDTREGALVYRLVLGPGHTPESYFDCWPPEHVVQNR
                     "
BASE COUNT          421 a          776 c          701 g          399 t
ORIGIN      
        1 cgtcgcgtat ccgccgcgcg gatgcgagat ccacgccgaa cggtttctcc gcgagcaggt
       61 ccttgccggc acgcaacacg tcgagataga tcgcctggtg cagatgatgc ggcaccgcga
      121 cgtaaaccac ctcgatgtcc ggccgtgcga gcagcacgcg atgatcggtg tcgaagtggc
      181 gcaccgtcgg cacctgacga aaccagtcga gcgccgccgc gttcacgtcg cacacgccga
      241 cgagctccgg ccgcaccggg cagtccgtca acgcgaacca gcggccaaag gcactggcga
      301 cttcgcggcc catcaggccg gcgccgatca cggcgacacg cacgggagcg gaggtgttca
      361 tgcgaggagt ttgagggctt cgcgcgcgga aacgccatca tgcacaaccg ccatcaaggc
      421 acgtgtgatc gcggcgggtt tcgagtgttg aatgatgttt cgtccgtaaa cgatgccgga
      481 agcgccctgc tcgatgagcg cctgcgtgcg cgcgagaatt tcctcctccg tcgccttgct
      541 gcctccgcgc acgagcaccg gaatgccccc cgcgatttcg acgaccttgt ggtaaaccga
      601 aatgtcgtcc gtcggatccg ccttgatgat atccgcgccg agctccactg cctgacgcac
      661 gagtggaatg attttttgcg gatcgccatc gaccatgtaa ccgccagctt tcgcattggg
      721 ctgaaacacg agcggctcga tcatcaacgg catgccgtag tgatcgcact ccggcttcag
      781 gtgcaggata ttttgcacac aatgctccgc cacctcagac tgaccgggaa tcgcaaacag
      841 gttgacgacg acgcaggtcg cgtcgagccg caccgcctgg agcacaggct gttcgatgat
      901 gcgtgagaac aacgtccgcg gcagctcgcg accgtaaacg ttggcgacgt cagtgcgcag
      961 caccagcgcg ggtttgccgc ggaccggcag cgcctgcaga tgcggcgcct gtccgacggt
     1021 cagttggatc gcatccggcg cggcggcgac gagcgcttcc accacgcgcg gcagattctc
     1081 gatgccggaa agaaaggagg cctcgttgaa gaagccgtga tcgatggcga catcgaagca
     1141 gcgccgcgtg gccggattaa agagacggtt gagtcgggcg gatttcatga atcgtaggca
     1201 tggatttaac caccgacggt gcccatcgcg ctacctctta acgcgcaaac accgacacta
     1261 tgatatcacc gcggcggcgg gaaacagcac ccggctgatc cccgaagcga ttcgccgcgc
     1321 gccaggagtg gtcgtcacca agcgcaacat cgatcagcgg cattgccagc cgggatgaaa
     1381 caacagggcc agagtctggg ccaataggcg tagcaccgag cctccggcgc ccgctagaat
     1441 cgtgcgcgac tccacgcgtt tacaatccat gggctcccct tcccgccttc tcgccaccgg
     1501 atacctcgct ttcgcatgga ttgcatccgc gccggctgcg tccgatctgg tgttgaacgg
     1561 ttcattcgaa cagaccgtcc acgatttccc cgcgggctgg agccgcctcg attccgccgt
     1621 tgcgggcgcg accgcgacgt ccgtcatcga cgtcggggtg cagggcgaaa aatccgtccg
     1681 tatcgatcgc accggagcga ccgtttctgc cggctccgcc ggccccggca tcgcccagca
     1741 cgatctggcg ctcaaacctg gcgaacgcta catgctgacg ttccaagccc gcggttcgcc
     1801 ggcgtcttcg tccgcgctgc gcgtttggat acagattcct ggtgccagcg agtttcctcc
     1861 cctgctcgcc gagtctgtgc cgctcacgac ggactggacg cgccatgaat tcggtttcga
     1921 gttccccgag gcagcgccgg ctggacgcgc cacactgcgc ttcgtggtga actcacctgc
     1981 cacggtgtgg ctcgacaaca tcgcgctctc cggcgccgca gctgccaagg ccgacatctc
     2041 ccgtccgcaa ccgcgtctgc ccgcatcgtc gagccgcaac ctcgtcccca acgcatcctt
     2101 tgaggccggc gccgacggtt ggctctccct cggaaaaccg ctcggcttcg gcggcaatgt
     2161 cgcgggcctc tatggggaca tcgtcggcga cgacacccgc gaaggggctc tcgtataccg
     2221 gttggtgctc ggcccggggc acacgcccga atcttatttc gactgctggc ccccggaaca
     2281 cgttgtgcaa aaccggc
//
LOCUS       DASPLT010000866         3069 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_11069, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000866 DASPLT010000000
VERSION     DASPLT010000866.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 3069)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3069)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3069
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_11069"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1070
                     /locus_tag="VEA63_09005"
     CDS             <1..1070
                     /locus_tag="VEA63_09005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD84180.1"
                     /translation="GFIGSRGDALFSPRLTTFLDVQVGDRIYAFAQMRVDRGFDPGRR
                     HLRGRLDEYAIRFTPWADGRVSAQIGKFGTVVGNWVPRHGSWENAFITAPLAYEHLTG
                     IWDSSAATSVEMLLDWAHVRPRPPRGAPATDKQLRLPLLWGPSYATGVAVSGETGRVN
                     YAVELKDAGLASRPVAWDRDNFELRNPTVSGRLGYRPNAMWHFGVSASRGGYLQPMAR
                     ATLPLGRAIDDYQHVVLAQDVSFAWHHWQVWAEFFESRFTVPGVGDADAFSYYVEAKY
                     KVTPQFAMAARWNEQHYGKLVDAVGDSVRWGANVRRLDLGPAYRFTPQVQLKLQYSVQ
                     HEEGAAREWSHLIAGQLTVRF"
     gene            1392..3020
                     /locus_tag="VEA63_09010"
     CDS             1392..3020
                     /locus_tag="VEA63_09010"
                     /inference="COORDINATES: protein motif:HMM:NF025295.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunoglobulin domain-containing protein"
                     /protein_id="HYD84181.1"
                     /translation="MKLSQSFHRRLHWINLPTTLLVALLQRTPVARVVTGAGEFVMSS
                     PLGAVLKSSVAAFASLGAVHSLAGATQLSTTAPSPFSATVGAAVTIGFSITGTLSEPE
                     TWTVGGSVPPGLTFNDGQTSGTIAGGTLVLSGTPTTAGSYSFSLSATDTPTGNSTPNY
                     NYTVNVTGAAAVAPAITMQPQSASGPAGSHLMLMAAASGTPAPAFQWQRNGSAVSGGA
                     DGTLMIDNTQPEHTGIYRVVATNSGGSSTSAPAILGVTTTAKVIGEGTELQPANIVHP
                     NGNVFDQVLLTGVAEAITADVAQRQVTRTSYIDLDDDIVQVEFSGPGTLSLVLDGSSA
                     PAAPTRYNQPSVSYMKGHAGIVITGATEQTNVSVFTVGRATAFDPTGTYNILLEPSAT
                     NVPASNGSSLFVGHQATSYDGIADIAFIAIASANGQFGGIRTSNAHYFAAKGFTGVYA
                     PGVTFNGPLFIGDVTASDDASPVIVVGAVTDSRITGGDLAQVNGKPVEVSGLTQLRFT
                     GGGDSHGNGLTAQQNQAVLMSNGQNVTAQVVVNP"
BASE COUNT          562 a          860 c         1082 g          565 t
ORIGIN      
        1 cgggtttcat cggctcgcga ggcgacgcgt tattcagccc gcggctcacg acctttctgg
       61 atgtgcaagt cggcgatcgc atttacgcat ttgcgcagat gcgggtcgac cgcggcttcg
      121 atccgggccg gcggcacttg cgcggccggc tcgacgaata cgcgatccga ttcacgcctt
      181 gggcggacgg ccgtgtgagt gcgcagattg gaaagttcgg cacggtggtg ggcaactggg
      241 tgccgaggca tggttcgtgg gagaacgcgt ttatcacggc gccgctcgcg tatgaacatc
      301 tcacgggcat ctgggacagc tcggcggcaa cgtcggtgga gatgctgctc gactgggcgc
      361 acgtgcggcc gcgtccgccg cgcggggcgc cggcgaccga caagcaactg cggctgccgc
      421 tgctctgggg accgagctat gcgacgggag tcgctgtatc cggagaaaca ggacgcgtga
      481 actacgcggt cgagttgaag gacgccggac tcgcatcgcg tccggtggcg tgggaccgcg
      541 acaatttcga gttgcgcaat ccgacggtga gtggacggct gggttatcgg ccgaatgcga
      601 tgtggcattt cggagtttcc gccagcagag gcggatactt gcagccgatg gcgcgggcaa
      661 cgcttccgct tggccgggcg atcgatgact atcagcacgt cgtgttggcg caggacgtga
      721 gcttcgcgtg gcatcactgg caggtgtggg cggaattttt cgaatcgcgt ttcacggtgc
      781 ccggagtggg cgacgccgac gcgttttcgt attatgtaga ggcgaaatac aaagtgacgc
      841 cgcagttcgc gatggcggcg cgctggaacg agcagcacta cggcaagctc gtcgacgcgg
      901 tgggcgacag tgttcgttgg ggggccaatg ttcggcgact cgatctggga ccggcgtatc
      961 ggttcacgcc gcaggtgcag ttgaaactgc aatacagcgt tcagcacgag gaaggggcgg
     1021 cgcgcgaatg gagtcatctc atcgcgggac agctcacggt gcggttttag agcacattca
     1081 aaaaagctgt agccgtttac ggatagcggc aggcgtctcg acatgatgcc tgccttggtc
     1141 gggtaggggg tgcgctcgcc gcaccacgtc gcgcgcgagc gcgcaaccta cacaggcagg
     1201 cacggaggcc cgccgctaca ttcggcggcc acgactttat ttaaaatgct gtagcgtcgc
     1261 gagggagagc ggcgagctga aggagtaaca ggcttgttac tgtgatctgc tttcgcggtg
     1321 ggagtgtccg cgcagttttc gggtgcgacc gaatctccgg aatttttcgg gcacggtctt
     1381 ttttcaccgc catgaaactg agccaatcct tccaccgccg tcttcattgg atcaacctcc
     1441 cgaccacgct tctggtggcc ttgctgcaac gcacaccggt cgcgcgcgtg gtcacgggtg
     1501 ctggagaatt cgtaatgtcg tcaccgctcg gtgcggtgtt aaaatcttcc gtcgcggcgt
     1561 tcgcgtcgct cggggcggtg cattcgctcg ccggtgcgac gcagttgtcg accaccgcac
     1621 cgagcccctt cagcgcgacg gtgggtgcgg cggtcacgat cgggtttagc atcacaggaa
     1681 cgttatccga gccggaaacc tggacggtgg gcggatcggt gccgccgggg ctgacgttca
     1741 acgacggaca aacgagcggg accatcgcgg ggggaacgct cgtgttgagc ggcacaccga
     1801 cgaccgcggg cagttattca ttttcactca gcgccaccga cacgccgacg gggaattcga
     1861 ctccaaatta caactacacg gtgaacgtaa cgggggcggc ggccgtcgcg ccggcgatca
     1921 cgatgcagcc acaaagcgcg agcggtccgg cgggcagcca tctgatgctg atggccgcgg
     1981 catcgggcac gccggcgccg gcgtttcaat ggcagcgcaa cggcagcgcg gtgagcggcg
     2041 gcgccgatgg gacgttgatg atcgacaaca cgcaaccgga gcacacggga atttaccgcg
     2101 tggtggcgac gaactcgggc ggctcgagca cgagtgcgcc ggcgattctg ggcgtgacga
     2161 cgacggcgaa agtgatcggc gagggcacgg agttacagcc ggcgaatatc gttcatccga
     2221 atggcaacgt gttcgatcag gtgttgttga ctggagtcgc ggaggcgatc accgcggatg
     2281 tcgcgcaaag gcaggtcacg cgcacgtcct acatcgatct cgacgacgat atcgtgcagg
     2341 tcgagttcag cgggccgggc acgttgtcgc tcgtgctcga cggatcgtcg gcgccggcgg
     2401 ctccgacgcg ttacaatcaa ccttcggtga gctacatgaa agggcatgct ggaatcgtga
     2461 tcacgggcgc gacggagcag acgaacgttt cggtgttcac cgtggggcgt gcgacggcgt
     2521 tcgatccgac tgggacctac aacatcctgc tggagccgag cgctacgaac gttcctgcca
     2581 gcaacggcag ttcgttgttt gtcgggcatc aagcgacgag ctatgacggt attgcggata
     2641 tcgcgttcat cgcgatcgcg agtgcgaacg gccaattcgg cggtatccga acttcgaatg
     2701 cgcactactt cgcggcgaag ggattcacgg gcgtgtatgc acccggcgtc acatttaacg
     2761 gaccgttgtt catcggcgat gtcacggcat cggacgacgc gagtccggtg attgtcgtcg
     2821 gtgcggtgac cgactcgcgc atcacgggcg gagatctggc gcaggtgaac ggaaagccgg
     2881 tcgaggtcag tgggctgacc caactgaggt ttaccggcgg cggcgattcg cacgggaacg
     2941 ggctcacggc gcagcagaat caggcggtgt tgatgagcaa cgggcagaac gtgacggcgc
     3001 aggtggtggt gaatccgtga cgtaggcgaa tcgtggacgc ccccgtggaa tggcgtcggc
     3061 tcgtcgggg
//
LOCUS       DASPLT010000867         2159 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_276062, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000867 DASPLT010000000
VERSION     DASPLT010000867.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 2159)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2159)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2159
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_276062"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..241
                     /locus_tag="VEA63_09015"
     CDS             <1..241
                     /locus_tag="VEA63_09015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD84182.1"
                     /translation="PNSAAALGMLVAIENKRDVAWYFLGPRAGGMELDVEGHHVLVLT
                     PQSPFGRELLGKHVGDTVRPPGRASSGDHRIAAVV"
     gene            complement(450..1031)
                     /locus_tag="VEA63_09020"
     CDS             complement(450..1031)
                     /locus_tag="VEA63_09020"
                     /inference="COORDINATES: protein motif:HMM:NF015506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HYD84183.1"
                     /translation="MAPPPSVEKTVIREVIFTPKEEEPPAPIADAETEASQAKGEREA
                     LRPTSEEPPPSPRLDNVFEMAAVKLPPSAGVAKIVPGAYGDPNGTGDAWMARPVLSYA
                     MLDNSPRTRAQAAPVYPSEARRQGLAGEVWVEFMVDEDGRVLNPRVVRSSDAMFESAT
                     LNAVLKWRFEPGRKGGKPVRFRMVAPVVFGLDA"
     gene            1557..>2159
                     /locus_tag="VEA63_09025"
     CDS             1557..>2159
                     /locus_tag="VEA63_09025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD84184.1"
                     /translation="MLARLSLLACAVVAAVSASAAIDPAIETALKAFRTEGPKGWSFT
                     QTTEADGQSRVERHDAAQPAFARWNLVQVDGRPPTADDIQDYNEKASRRSRAGTAPRL
                     NDQIDLATITVAHETPERTTYRARLKQGEDGDATAKFLVATLVLHKPTSTIEIFEIAS
                     TEPFSPVLGVNIAEMKTVMSYSLPESDRPSLLQKSTTRLRG"
BASE COUNT          420 a          798 c          556 g          385 t
ORIGIN      
        1 tcccaactcc gccgctgcgc tcggcatgct tgtcgccatc gaaaacaaac gcgacgtcgc
       61 ctggtatttt ctcggcccac gcgccggtgg catggaactc gacgtcgaag gccatcacgt
      121 gctcgtcctc accccgcaat cgcctttcgg tcgcgaactc ctcggcaaac acgtcggcga
      181 caccgttcgc ccgcccgggc gcgccagcag cggcgaccat cgcatcgcgg ccgtcgtcta
      241 acccgcattg atcacacgca gatgtggggt gtgtgcagtc tgttggagcg aaacgcgtga
      301 gcgtttcatc cgggcgagag acgaaacgct cacgcttttc gctacaggtg tgaatttttc
      361 gggccagacc gctcgcagca aacggcgccc tcgaggctgt cggccgttcg cagccctcgc
      421 cgcgattcgc gcccgaccgc gccatctcgc tacgcgtcca atccaaacac caccggcgcg
      481 accatccgaa aacgcaccgg tttcccgccc ttgcgccccg gctcgaaccg ccactttagc
      541 accgcgttca acgtcgcact ctcgaacatc gcgtcgctcg aacgcaccac gcgcggattg
      601 agcacgcgtc cgtcctcatc gaccatgaac tcgacccaca cctccccggc gagcccttgc
      661 cgccgcgcct cactcggata aaccggggca gcctgcgcgc gtgttcgcgg cgagttgtcg
      721 agcatcgcgt aactcaaaac cggccgcgcc atccacgcat cgcccgtgcc gttcggatca
      781 ccataggcgc ccggcacgat cttcgccaca cctgcgctcg gcggcagctt caccgcagcc
      841 atctcgaata cattgtccaa ccgcggtgaa ggtggcggct cctcggaagt cggccgcaac
      901 gcctcgcgct cgcctttggc ctgcgacgcc tccgtctctg cgtcggcgat cggcgctggt
      961 ggctcctcct ctttcggcgt aaaaatcacc tctcgaatca cggttttctc caccgacgga
     1021 ggcggcgcca cggagtgtga gggacgatgc gagaccaaca agacgagcgc atgcagcgcc
     1081 gtcgcaacca tcaccggaac cagaaaacgc gtgtgcatat gaacctccag gttgagcttt
     1141 catctccacg acgcgccaac cgcctgtttc ttagcgggaa taattcattt gaaccgcgaa
     1201 tgtcccgaat cgacacgaat gaaaatcgag cctgacgcag ctctcatcgg gaaccgaacg
     1261 ttgatcgcac gaccagccca aacgctttcc atcaactccg ctgcattcgt gtcccttcgt
     1321 gtccattcgt agttcaactc atccccttcc gcgcttcgcc gatcaggcga agcacgcacg
     1381 gcttcctccg ctttccagct ccgaaaaaat tagcgcagat tagcggttaa aaaatccggc
     1441 aagcttctgc gtccaacccc gatgcatcca tccgcggtta aaggacgctt tcattcgcgc
     1501 ccgttcgcgt gcattcgtgt ttcgtcatgg catcgtcgcg cttccgtttt ccactcatgc
     1561 tcgcacgcct ttctctcctc gcctgcgccg tggtcgccgc cgtgtccgct tccgccgcca
     1621 tcgatcccgc aatcgaaact gcgctcaaag ccttccgcac cgaaggcccg aaaggctggt
     1681 cgttcacgca aaccaccgaa gccgacggcc agagccgcgt cgaacgccac gacgccgcgc
     1741 aacccgcgtt cgctcgctgg aacctcgtcc aagtcgacgg ccgcccaccc acggccgacg
     1801 atatccagga ctacaatgaa aaagccagcc gccgctcgcg cgccggcact gctccccgac
     1861 tcaacgatca aatcgatctc gccacgatca ccgtcgcgca cgagacaccc gaacgcacga
     1921 cctaccgcgc ccgcttaaag caaggcgaag acggcgacgc gaccgcgaag ttcctggtgg
     1981 cgacgctcgt gttgcacaag ccgacttcga cgatcgagat cttcgagatt gcgtcaacgg
     2041 agcccttctc tcccgtcctc ggcgtgaaca tcgccgagat gaaaaccgtc atgtcctaca
     2101 gcctgcccga aagcgaccgg cccagcctcc tccaaaaatc gacgacgcga ctccgcggt
//
LOCUS       DASPLT010000868         3228 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_112005, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000868 DASPLT010000000
VERSION     DASPLT010000868.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 3228)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3228)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3228
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_112005"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..643)
                     /locus_tag="VEA63_09030"
     CDS             complement(<1..643)
                     /locus_tag="VEA63_09030"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HYD84185.1"
                     /translation="MTTTSSLAPLFIAGEWLRPTQLATSAVFNPSTGETIAQTPLAGV
                     AEVDAAVSAAHAAFPGWWQTPAIERARVLFRYRELLLSHFDEIAALISREHGKTRAEA
                     RGDLQRGIEVVEFATGVPTLLFGDTLENVARGIDCETLRQPLGVCVGITPFNFPAMVP
                     MWMFPIALACGNTFVLKPSEKVPLTAVRIVELLAEAGLPKGVLNLVHGGRDCVD"
     gene            complement(715..1776)
                     /locus_tag="VEA63_09035"
     CDS             complement(715..1776)
                     /locus_tag="VEA63_09035"
                     /inference="COORDINATES: protein motif:HMM:NF013116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase family protein"
                     /protein_id="HYD84186.1"
                     /translation="MTPSRMRQMTALGADWWNDSGVPAELAEAVSLGAVGGTSNPVIV
                     SQAVKAQPALCEPLVDRFVLENVSDCEDEIAWRLIHALAIEAAGKLRPVYDRTRGEKG
                     FLSVQVNPMYYRCKTRMVEQATQLASLAPNIAIKAPATEIGIAAIEEMTARGIRVNAT
                     VSFTVPQAIAVAQALERGLKRARAAGHDADRIRPYITLMIGRVDDHLKRVADKQKIAV
                     TAGVLDWAGIAVFKHAHRIFKDRGYPGTLLAAAYRHEGHWSQIIGRDVLQTIPYTWWT
                     QFNVSAIEPRLTLDEPVDERIVAELRAKFPDFIRAHDEAGMRPSEFSTYGATVHTLHQ
                     FLCGYADLMAQVRARMLPL"
     gene            complement(1789..2694)
                     /gene="iolE"
                     /locus_tag="VEA63_09040"
     CDS             complement(1789..2694)
                     /gene="iolE"
                     /locus_tag="VEA63_09040"
                     /EC_number="4.2.1.44"
                     /inference="COORDINATES: protein motif:HMM:TIGR04379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050114 - myo-inosose-2 dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="myo-inosose-2 dehydratase"
                     /protein_id="HYD84187.1"
                     /translation="MNVQLHRDCLVGANPIIWSNDDFAELAGDVPLDTILREMRAAGY
                     AGTELGHAYPRSPAALAEPLARHRLRLASGWHSSYLASRELAAEEETFRAHLGLLKTL
                     GARVAIVAECTRCIHGTRAVALGFGEGAQPLLNDDEWARFTSGCERLIAIADDEEMKV
                     VYHHHMGTVIQREAELERLLADVPPLSLLLDPGHLAFAGIDPVAVARRYATRIGHVHL
                     KSVRPAVVERARNEGWSFHRAVREGVFTIPGDGSVDFPAIFRVLGDAAYRGWLIAEAE
                     EDPAKAPAFPKAKAARAYVRAHAGI"
     gene            complement(2694..>3228)
                     /locus_tag="VEA63_09045"
     CDS             complement(2694..>3228)
                     /locus_tag="VEA63_09045"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HYD84188.1"
                     /translation="MGYEIAGALGVKLAEPKREVYAFLGDGSYLMLHTEIVTAVQEGL
                     RLTIVLVDNHGFGCIHGLQRGCGGNSFGNEFRSRVAASGRLEGAPVPVDFVKNAESLG
                     ATAVRANTPEELRTALADARRAKGVRLICVETPADVRLPGYSWWDVPIASTSSVPAVG
                     AARRAYDRARKKQRLYY"
BASE COUNT          578 a         1119 c          989 g          542 t
ORIGIN      
        1 cgtcgacgca gtcgcgcccg ccgtgcacga ggttgagcac gcctttcggc agcccggctt
       61 cggcgagcag ctcgacgatg cgcaccgcgg tcagcgggac tttttcgctg ggcttcagga
      121 cgaacgtgtt cccgcacgcg agtgcgatcg gaaacatcca catcgggacc atcgccggaa
      181 aattgaacgg cgtgatcccg acgcacacac cgagcggctg gcgcagcgtt tcgcaatcga
      241 tgcctcgcgc gacgttctcg agcgtgtcgc cgaaaagcag cgtcggcacg ccggtcgcga
      301 actcgacgac ttcgatgccg cgttgcaaat caccgcgcgc ctccgcgcgc gtcttgccat
      361 gctcgcgcga gatcagcgcg gcgatctcgt cgaaatgcga caggagcaac tcgcgataac
      421 gaaacagcac gcgcgcgcgc tcgatcgccg gcgtctgcca ccagcctgga aacgccgcat
      481 gcgccgccga caccgccgcg tcgacttccg cgactccggc gagcggcgtc tgcgcgatcg
      541 tttcgcccgt ggaagggttg aacacggccg aggtcgcgag ctgcgtcggc cgcaaccatt
      601 cgccggcgat gaagagcgga gcgagagaag aggtggtggt cataaagtag ggcgggcttc
      661 agcccgcttt cgaaaaatgc agagcgtcga gagcgggctg aagcccgccc cactctacag
      721 cggcagcatt cgcgcgcgca cttgcgccat caggtccgca tagccgcaga gaaattggtg
      781 gagggtgtga accgtggcgc cgtaggtcga aaactccgag ggcctcatgc cggcctcgtc
      841 gtgtgcgcgg atgaaatccg gaaacttcgc gcgcagctcg gcgacgatcc gttcgtcaac
      901 gggttcatcg agcgtgagcc tcggctcgat ggcggaaacg ttgaactgcg tccaccacgt
      961 gtaaggaatc gtctgcaaca cgtcacgccc gatgatctgc gaccaatggc cttcgtgacg
     1021 gtaagccgcg gccagcagcg tgccgggata accgcggtcc ttgaaaatac gatgcgcgtg
     1081 cttgaacacc gcgatgcccg cccaatcgag cacgccggcc gtcactgcga tcttttgctt
     1141 gtcggcaacg cgtttcaaat gatcgtccac ccggccgatc atcagcgtaa tgtaaggtcg
     1201 gatacggtcc gcgtcgtgcc ccgctgcacg cgcccgcttc agtccgcgct cgagcgcctg
     1261 cgccacggcg atcgcctgcg gcaccgtgaa gctcacggtc gcgttcacgc gaatcccgcg
     1321 cgcggtcatt tcctcgatcg ctgcgattcc gatctcggtc gccggcgcct tgatcgcgat
     1381 gttgggcgcc agcgatgcga gctgtgtcgc ctgctcgacc atccgcgtct tgcaccggta
     1441 atacatcgga ttcacctgga ccgaaaggaa ccccttctcg ccgcgcgtgc ggtcgtaaac
     1501 cggccgcagt tttcccgcgg cctcaattgc gagcgcatgg atcaagcgcc acgcaatctc
     1561 gtcctcgcag tccgacacgt tctccagcac gaagcgatcg accagcggct cgcagagcgc
     1621 cggctgcgcc ttcaccgctt gcgagacgat caccggattc gatgtgccgc cgaccgcccc
     1681 gaggctcacc gcctccgcga gttccgcggg cacgcctgag tcgttccacc aatcggcgcc
     1741 gagcgccgtc atttgccgca tgcgagaagg agtcatgacg attgcagatt aaatgccggc
     1801 gtgcgcgcgg acgtaggcgc gcgccgcttt ggctttggga aatgccggcg ctttcgcggg
     1861 atcctcctcc gcttcagcga tcagccagcc gcgataagca gcgtcgccca gcacgcggaa
     1921 gatcgccgga aaatccacgc tgccgtctcc cgggattgtg aaaacaccct cgcgcacggc
     1981 gcgatggaac gaccagcctt cgttccgcgc gcgctcaacc acggcgggac gcacgctctt
     2041 tagatgcacg tgcccgattc gcgtcgcgta tcgccgcgcg acggcaaccg gatcgatccc
     2101 cgcgaaggcg agatgtccgg gatcgagcag caacgacaac ggcggaacgt ccgcgagcag
     2161 ccgttcgagc tccgcctcgc gttggatcac cgtgcccatg tggtggtgat aaacgacttt
     2221 catttcctcg tcgtccgcga tcgcgatgag ccgttcgcat ccactcgtga aacgcgccca
     2281 ttcgtcatcg ttaagcagcg gctgagcacc ctcgccaaag ccgagcgcca ccgcccgcgt
     2341 gccgtggatg caacgcgtgc attccgccac gatcgccacg cgcgcgccca acgtcttcag
     2401 cagaccgagg tgcgcgcgaa atgtctcttc ttccgcggcg agttcgcgcg aggcgagata
     2461 ggagctgtgc cagccgctcg ccaggcgcag acgatgacgc gcgagcggct cggccaacgc
     2521 cgccggcgag cgcggataag cgtgccccag ttccgtgccg gcgtaacccg ctgcgcgcat
     2581 ctcgcgcaaa atcgtgtcca acggcacatc gcccgcgagc tccgcaaaat catcgttgct
     2641 ccagatgatc ggattcgcgc cgaccagaca gtcgcgatgg agttgaacgt tcatcagtag
     2701 tagagccgtt gtttcttccg cgcccgatcg taggcgcgcc gcgccgcgcc caccgccggc
     2761 acgctcgacg tcgacgctat cggcacgtcc caccacgagt agccgggcaa ccgcacgtcc
     2821 gccggcgttt cgacgcagat gagccgcacg cctttcgcgc gccgagcatc tgctagcgcg
     2881 gtgcgcaact cctcgggcgt attcgcgcgc accgcggttg cgccgaggct ctctgcattt
     2941 ttaacgaagt cgaccggcac cggcgcgcct tcgagccgcc cgctcgccgc gacgcgcgac
     3001 cggaattcgt tgccaaaact attccctcca cagccgcgct ggaggccgtg aatgcagccg
     3061 aagccgtgat tgtcgacgag cacgatcgtc agtcgcaggc cttcctgcac cgcggtcacg
     3121 atctccgtgt gcaacatcag ataactgccg tctccaagaa acgcatacac ctcgcgcttc
     3181 ggctccgcga gtttcacgcc gagcgcgccg gcgatttcgt agcccatg
//
LOCUS       DASPLT010000869         1812 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_91870, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000869 DASPLT010000000
VERSION     DASPLT010000869.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1812)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1812)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1812
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_91870"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..665
                     /locus_tag="VEA63_09050"
     CDS             <1..665
                     /locus_tag="VEA63_09050"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HYD84189.1"
                     /translation="IDCSLSSEANFREGLLGENGEGGAWVRQAKGGTLFLQHLQCLSE
                     SVQKELVSVLRNTAHGFRLVCTTTEDLEALTDEGKFHDELFYRVASLPVQMPPLRDRI
                     EDIPLLVKNYASKAANPHFDASLVEFTDDAFAVLTAYHWPGNLTELYQVISKIAATTE
                     TRIITSQQLPLRLRELKNWPTLAEYLAGQEKQYRDMVLHACRGDTAAAAKVLGVDEAV
                     LR"
     gene            824..>1812
                     /gene="carB"
                     /locus_tag="VEA63_09055"
     CDS             824..>1812
                     /gene="carB"
                     /locus_tag="VEA63_09055"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007358665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HYD84190.1"
                     /translation="MPKRPDLKSILIIGAGPIVIGQACEFDYSGTQACKALKEEGYRV
                     ILVNSNPATIMTDPEFADVTYIEPLNVEILEKIIAAERPSALLPTLGGQTALNLSMEL
                     NKAGVLEKYGVEMIGAKPEAISKGEDRELFKQCMLKIGLDVAKSRTVKSMDEARAAAE
                     MLGVYPLIIRPSFTLGGSGGGIAYNKEEFERIVANGLDLSPVHEVLVEECLLGWKEYE
                     MEVMRDHKDQCVVICSIENFDPMGVHTGDSITVAPAMTLTDKEYQVMRDASFAVIREI
                     GVETGGSNIQFSVDPDSGRMVVIEMNPRVSRSSALASKATGFPIAKIAAKLAVG"
BASE COUNT          364 a          514 c          600 g          334 t
ORIGIN      
        1 gcatcgattg ctcactgagt tcggaagcga atttccgcga agggctgctc ggcgaaaacg
       61 gcgaaggtgg cgcgtgggtg cggcaagcga agggcggcac gcttttcctg cagcatttgc
      121 agtgtctgtc ggagagcgtg cagaaggagc tcgtgagcgt cttgcggaac acggcgcacg
      181 gtttccggct cgtttgcacg acgacggagg acctcgaggc gctcaccgac gaagggaaat
      241 ttcacgacga gcttttctat cgcgtggcgt cgctgccggt gcagatgccg ccgctgcgcg
      301 atcgcatcga ggacataccg ctactggtaa aaaattacgc gagcaaggcg gcgaatccgc
      361 acttcgacgc gagcctggtg gagttcacgg acgacgcgtt cgcggtgctt acggcatatc
      421 actggccggg caatctcacg gaactctacc aagtcatttc gaaaatcgcg gcgacgacgg
      481 agacgcggat tatcacgtcg caacagctgc cgttgcggct gcgcgaattg aagaactggc
      541 ccacgctggc cgagtatttg gccggacagg aaaaacagta tcgcgacatg gtgttgcatg
      601 cgtgccgcgg cgacacggcg gccgcggcga aggtgcttgg cgttgacgaa gcggtgttgc
      661 ggtagcgagt gagcctcgga gggcctgcac acgggcgatc tgacgaggcg ccggatgtgg
      721 cgagaagcgg tggtgataat gcgcgagggg cgattggctg ctcgccacgt ttcccgcttg
      781 ccgcgcgatt cgacgcgctc aactctcggc caaatctacg ttcatgccca aacgtcccga
      841 ccttaagtcc atcctgatca tcggcgccgg ccccatcgtc atcggccagg catgcgaatt
      901 cgactattcc ggcacgcaag cctgcaaggc gctgaaggag gagggttacc gcgtcattct
      961 cgtgaactcg aatccggcga cgatcatgac ggatccggag ttcgccgatg tgacctacat
     1021 cgagccgctc aacgttgaga ttctcgaaaa gatcatcgcg gccgagcggc cgtccgcgct
     1081 gctgccgacg ctcggcggtc agacggcgtt gaatctctcg atggagctga acaaggcggg
     1141 cgtgttggag aaatacggcg tcgagatgat cggcgcgaaa cccgaggcga tttcgaaggg
     1201 cgaagatcgt gagttgttca agcagtgcat gctcaagatc ggtctcgacg tcgcgaaatc
     1261 gcgcacggtg aaatcgatgg acgaagcgcg cgcggcggcg gagatgctcg gcgtgtatcc
     1321 gttgatcatc cggccgtcgt tcacgctcgg cggtagcggc ggcggcatcg cttacaacaa
     1381 ggaggagttc gagcggatcg tcgcgaacgg cctcgatctc tctccggttc acgaggtgct
     1441 cgtcgaagag tgtctgctcg gttggaaaga atacgagatg gaggtgatgc gcgatcacaa
     1501 ggatcagtgc gtcgtgatct gctcgatcga gaacttcgat ccgatgggcg tgcacaccgg
     1561 tgactcgatc acggtcgcgc cggccatgac gctcaccgac aaggaatatc aggtgatgcg
     1621 tgacgcctcg ttcgcggtga ttcgcgaaat cggcgtggag acaggcggct ccaacatcca
     1681 gttctccgtc gaccccgaca gcggccgcat ggtcgtcatc gaaatgaatc cgcgcgtgag
     1741 ccgcagctcg gcgctcgctt ccaaggccac cggctttccc atcgccaaga tcgcggcgaa
     1801 actggccgtc gg
//
LOCUS       DASPLT010000870         1497 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_276104, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000870 DASPLT010000000
VERSION     DASPLT010000870.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1497)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1497)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1497
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_276104"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..411)
                     /locus_tag="VEA63_09060"
     CDS             complement(<1..411)
                     /locus_tag="VEA63_09060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase substrate-binding
                     domain-containing protein"
                     /protein_id="HYD84191.1"
                     /translation="MAVEKISSLQNPRVKQLVKLRDRRPRDEAGVFLVEGYREIRRAL
                     EKDVVLREIYFSPDWFLGENEPALIEQVRNAGAQAFELSKDAFAKVAYRERPDGLLAV
                     APQWRTTLADLDAIAGRGLRTPPSERAVTQHAPAG"
     gene            487..>1497
                     /locus_tag="VEA63_09065"
     CDS             487..>1497
                     /locus_tag="VEA63_09065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAM domain-containing protein"
                     /protein_id="HYD84192.1"
                     /translation="MCFVVKKKKSFNDQPFAYHQEIELEISTLTNLGSGLGRVQIENP
                     ESKIENSSGWVVMVPFTLPGERVRARVFRNHKNFSEADLVSVLTPSPHRVAPGCPLFG
                     RCGGCQYQHLTYNEQLKWKQQQVAELLQHMAGIEFPVSPVVPSPRDYGYRSKITPHFA
                     APRRFTRDTPGSASRVLTSESQEQSVPGAVSDPNSELRSSISDPASAGFAIGFLRQGT
                     RFDIVDVPRCPIATDAINDRLTEVRARVHENRETYTRGATLLLRDASGTVTTDYDAIV
                     TETVGDLKLHFLARDFFQNNPFILPAFTRYVREQAAASGARFLVDAYCGSGLFALSAA
                     SAF"
BASE COUNT          266 a          494 c          438 g          299 t
ORIGIN      
        1 ccccgctggc gcgtgctggg taacggcgcg ctcgctcggc ggggttcgga gaccccgccc
       61 tgcaatcgcg tcgagatccg cgagggttgt cctccattgc ggagcgacgg ccagcaatcc
      121 atccggtcgt tcgcgataag cgactttcgc gaacgcgtcc ttcgacagct caaatgcttg
      181 cgccccggcg ttgcgcacct gctcgatcag cgcgggttcg ttttcgccga ggaaccaatc
      241 gggcgaaaag taaatctctc gcaacacgac gtccttctcg agcgcccgcc ggatctcgcg
      301 gtaaccctcg acgagaaaca cgcccgcctc gtcgcgtggc cgccgatcgc gcagcttcac
      361 gagctgcttc acgcggggat tttggaggct ggaaattttt tcgacggcca cggaggagaa
      421 atcaccacga aacacagcaa acacacgaaa gaaaaccatc tcgtttccgg ccctttcgtg
      481 tatttcgtgt gtttcgtggt taagaaaaag aaatcgttca acgaccagcc attcgcctac
      541 catcaggaga tcgagctcga gatcagcacg ctcacgaacc tcggttcggg cctcggtcgc
      601 gtccaaatcg aaaatccgga atcgaaaatc gaaaattcct cgggctgggt cgtcatggtg
      661 ccgttcacgc tgccaggcga gcgcgtgcgg gcgcgcgtgt ttcgcaatca caagaatttc
      721 tccgaagccg atctcgtttc cgtgctcaca ccgtcgccgc atcgcgtcgc gcccggctgt
      781 ccgctcttcg gccgctgcgg cggttgtcag tatcagcatc tcacctacaa cgagcagctg
      841 aaatggaaac agcagcaggt cgccgaactc ctgcagcaca tggcgggcat cgagtttccc
      901 gtttccccgg tcgtgccgtc gccgcgcgat tacggctatc gttcgaaaat cacgccgcac
      961 ttcgccgcgc ctcgacgctt cacgcgcgat acgcccggtt ccgcgtcccg cgttttgacc
     1021 tccgagtcgc aggagcagtc cgttcccggc gcggtttcgg acccgaactc cgaactccgg
     1081 tcttcgattt ccgatcccgc atccgcggga tttgccatcg gcttccttcg ccagggcacg
     1141 cgtttcgaca tcgtcgacgt gccgcgctgc ccgatcgcga ccgacgcgat caacgatcgt
     1201 ctcactgaag ttcgcgcgcg cgtgcacgag aatcgcgaga cctacacgcg cggcgcgacg
     1261 ctcctcctac gcgacgcctc gggcacggtg acgacggact acgacgcgat cgtcaccgag
     1321 acggtgggcg acttgaagct gcactttctc gcgcgcgatt tttttcagaa caatccgttc
     1381 atcctgccgg cgttcacgcg gtatgttcgg gagcaggcgg cggcgagcgg cgcgcggttt
     1441 ttggtcgatg catattgcgg gagcgggctg ttcgcgttgt cggcggcgtc cgcgttc
//
LOCUS       DASPLT010000871         1900 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_256551, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000871 DASPLT010000000
VERSION     DASPLT010000871.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1900)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1900)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1900
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_256551"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(231..854)
                     /locus_tag="VEA63_09070"
     CDS             complement(231..854)
                     /locus_tag="VEA63_09070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD84193.1"
                     /translation="MTLLRNPFVTAAAGLLISVALGVFMFWRAAQPLVAQLAETRAKA
                     VAALPKKEKGWDFWTIEIENLSNELKEERARLAQQTDLLEQREARIAAEQKELATMRA
                     EIEALRQEIGDKVVMIRADESKNVRSLAQTYANLTPRAAVAIIRELDDVTAVKILFLM
                     KPDIVGPIFEEMSKTSGSDGTPLARRAAILSEKLRLMKVNTSSPNPS"
     gene            complement(851..1306)
                     /locus_tag="VEA63_09075"
     CDS             complement(851..1306)
                     /locus_tag="VEA63_09075"
                     /inference="COORDINATES: protein motif:HMM:NF014144.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar FliJ family protein"
                     /protein_id="HYD84194.1"
                     /translation="MKRFRFPLRPVAVLRAHQEVRAREVFAAAVHACVQAEEELARTR
                     VRMRALEAALFSGRKETFRAAEAALLLSDYRRECAAEGEMERRVIAARDEMQKRRTDY
                     VEAHRKVEVVQRLETKARTSHRLENDRAEQAEFDDYASRRRTKPFSLAL"
     gene            complement(1303..>1900)
                     /locus_tag="VEA63_09080"
     CDS             complement(1303..>1900)
                     /locus_tag="VEA63_09080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009507949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="EscN/YscN/HrcN family type III secretion system
                     ATPase"
                     /protein_id="HYD84195.1"
                     /translation="AIAEAWRDAGKNVLLMMDSVTRFAMAQREIGLAVGEPPATRGYT
                     PSVFAMLPRLLERAGAGEKGAITALYTVLVEGDDMNEPVADAVRGILDGHIVLSRHLA
                     HFNHYPAIDVLESVSRLTREVCTPDQVSLVGRAREQLALYRKNEDLVSIGAYQKGSNA
                     ALDRAIALHDPLRNFLRQSVEEHTPRAQSFLNLKQILA"
BASE COUNT          340 a          576 c          629 g          355 t
ORIGIN      
        1 tcaacggcgt cgggtgagcc agtctcggct tccgacaaca agagatcgcc gaactcgagc
       61 gcgtcttcag cgctggagga agcagtcgcg ccgggaatga cgccgacgtc ggcggcagtc
      121 gggctggtca ggccgggatc gagaggaaaa gtcgaagggg aaagttgcat gcatagttga
      181 accctccgtg gtgagaaatc gcgccgatcc ttggcacgat ggtaaaacga tcaggatgga
      241 ttgggagagg aggtgttaac cttcatgagt cgcagcttct cggagaggat cgccgcgcgg
      301 cgtgcgagcg gcgtgccgtc gctgccgctc gtcttgctca tctcttcgaa aatcgggccg
      361 acgatgtccg gtttcatcag gaaaagaatt ttcaccgcgg tgacgtcatc gagctcgcga
      421 atgatcgcga ccgccgcgcg cggcgtgaga ttcgcgtaag tttgtgcgag cgagcggacg
      481 ttcttggatt cgtcggcgcg aatcatgacg accttgtcgc cgatttcctg gcgcagcgcc
      541 tcgatctcgg cgcgcatggt cgcgagctcc ttctgttcgg cggcgatgcg agcttcgcgc
      601 tgctcgagca agtccgtctg ttgcgcgagg cgggcgcgct cctccttcaa ctcgttggaa
      661 aggttttcga tttcgatcgt ccagaagtcc cagccttttt ctttctttgg caacgccgcg
      721 accgccttgg cgcgcgtttc cgcgagctgc gcgaccagcg gctgcgctgc gcgccagaac
      781 atgaacacgc cgagcgcgac ggaaatcagg agaccggccg cggctgtgac gaagggatta
      841 cgcaaaaggg tcatagcgcg agcgagaagg gtttggtgcg gcggcggctg gcgtagtcgt
      901 cgaattccgc ctgctcggcc cgatcgtttt cgagacggtg cgaggtgcgg gctttcgtct
      961 cgagacgttg gacaacttcg accttgcggt gcgcttcgac ataatcggtg cggcgcttct
     1021 gcatttcgtc gcgcgcggca atgacgcgcc gttccatctc gccttccgcg gcgcattcac
     1081 ggcggtaatc ggaaagcagc agcgccgcct cggcggcacg aaacgtttcc ttgcgaccgg
     1141 aaaacagcgc ggcttcgagc gcacgcatcc ggacccgcgt gcgcgccagt tcctcctcgg
     1201 cttggacaca ggcgtgcacc gcggcggcga acacctcgcg cgcgcggact tcctggtgag
     1261 cgcgcaaaac cgcgaccgga cgaagaggga atcggaagcg cttcatgcga ggatctgttt
     1321 gaggttgaga aaactctgcg cccgcggggt gtgttcctcg accgactggc gcagaaaatt
     1381 ccgcagcgga tcgtgcagcg cgatcgcgcg atcgagcgcg gcgttcgagc ctttttgata
     1441 cgcgccgatg gaaacgaggt cctcgttttt ccgatacaac gcgagctgct cgcgcgcgcg
     1501 gccgacgagc gaaacctggt ccggcgtgca gacttcgcgc gtcaaacggc tcacgctttc
     1561 caacacgtcg atcgcgggat agtggttgaa gtgcgcgaga tggcgcgaaa gcacgatgtg
     1621 tccgtcgaga ataccgcgca cggcatcggc aaccggttcg ttcatgtcgt caccctcgac
     1681 gagcacggtg tagagcgcgg tgatcgcacc cttctcgccc gcgcccgcgc gttcgagcaa
     1741 ccgcggcagc atcgcaaaaa cggaaggcgt gtagccgcgc gtcgccggcg gttcgccgac
     1801 ggcgaggccg atttcacgtt gggccatcgc gaaacgtgtc accgagtcca tcatcagcag
     1861 cacgttcttg cccgcatcgc gccacgcctc ggcgatcgcg
//
LOCUS       DASPLT010000872         1115 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_256571, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000872 DASPLT010000000
VERSION     DASPLT010000872.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1115)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1115)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1115
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_256571"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..470)
                     /locus_tag="VEA63_09085"
     CDS             complement(<1..470)
                     /locus_tag="VEA63_09085"
                     /inference="COORDINATES: protein motif:HMM:NF028046.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD84196.1"
                     /translation="MRDEIERSMREKVLLFAERVQTDNEHSVQEIVNSAAKAADARAT
                     VVDSKGVVLADSEAHAATMENHAYRPEFQQAFAGNIGMASRRSQSVGVDFLYVAAPTP
                     DGAVRLAYPLRAIAETTRQIRRALLISSAFAFIIAAVVAAITASAISRRLQKIVA"
     gene            complement(572..>1115)
                     /locus_tag="VEA63_09090"
     CDS             complement(572..>1115)
                     /locus_tag="VEA63_09090"
                     /inference="COORDINATES: protein motif:HMM:NF012698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HYD84197.1"
                     /translation="LLDIMVPGGDGFELCRRIRQAQSVAMTPVIFLTAKTGESDRVLG
                     LELGADDYITKPFSPRELVARVKAVLRRFERPLAPSVVHAGEVEIDAGAMTLTVRGKQ
                     VPTTATEFRLLDYLARHPGRVFTRDQLLDAVWRDTAFVTPRSVDVYVRRIREKIEQDP
                     EEPRLLKTVRGAGYRFEAPK"
BASE COUNT          222 a          346 c          340 g          207 t
ORIGIN      
        1 gcgacgatct tctgcaatcg gcgggaaatt gccgatgctg tgatggccgc caccacagcg
       61 gcgatgatga acgcaaatgc cgagctgatg agaagtgcac gacggatctg gcgcgtggtt
      121 tccgcgatgg ctcgtagtgg ataggcgagg cgaaccgctc cgtccggagt aggcgccgcg
      181 acgtagagga aatcaacccc gaccgattgg cttcgccgac tcgccatacc gatgttgccc
      241 gcaaacgcct gctgaaactc tggccgatag gcatggttct ccatagttgc cgcatgagct
      301 tccgagtcag caagcaccac gcctttggaa tcgacgacgg tggcacgagc gtccgccgct
      361 ttcgcagcag aattcacaat ctcctggacc gagtgctcgt tgtccgtctg cacgcgctcc
      421 gcgaaaagca gcaccttttc ccgcatcgag cgctcgatct cgtcgcgcaa cgagcgttcc
      481 caataccgcc ggacgctgaa gtcgagcgtc agggtgcaga cggcgatgac cagcaggaat
      541 gccgcggcga gcttaaggaa gatccggctt ttcacttggg tgcctcaaac cggtagcccg
      601 cgccgcgaac ggtcttcagc aatcgcggct cctcgggatc ctgctcaatc ttctcgcgga
      661 tgcgccggac gtatacgtcc actgagcggg gcgtgacgaa ggctgtgtct cgccaaaccg
      721 catcgagcag ctgatcgcga gtaaaaacgc gcccgggatg gcgtgcgaga taatcgagca
      781 gccggaattc tgtcgccgta gtcggcactt gcttaccacg cacggtcagc gtcatggctc
      841 cagcgtcaat ttcgacttcg ccggcgtgca ctacggacgg cgcgagtggg cgctcgaagc
      901 gtcgcagtac ggccttcacg cgcgctacca gttctctggg actgaacggc tttgtaatgt
      961 agtcgtccgc gccgagttca agccccagca cgcgatcgct ctcacccgtt ttcgcggtca
     1021 gaaagatgac tggtgtcatc gccacagact gcgcctggcg aatgcggcgg cagagttcaa
     1081 acccgtcccc gccagggacc atgatgtcga gaagg
//
LOCUS       DASPLT010000873         1526 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_34286, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000873 DASPLT010000000
VERSION     DASPLT010000873.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1526)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1526)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1526
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_34286"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            254..715
                     /locus_tag="VEA63_09095"
     CDS             254..715
                     /locus_tag="VEA63_09095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four-helix bundle copper-binding protein"
                     /protein_id="HYD84198.1"
                     /translation="MNLIETMLRSHPRADLQRVEKYADVLNAISLCSQICTSCADACL
                     AEDNSAHLRRCISTDMDCADVCDATARLVMRQTESPYELVHAQLHACILACQICADEC
                     EKHASMHDHCRICAETCRHCQERCNFLLGEISSSGVEEESIDPTDSPSFRR"
     gene            736..>1526
                     /locus_tag="VEA63_09100"
     CDS             736..>1526
                     /locus_tag="VEA63_09100"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HYD84199.1"
                     /translation="MSDTEVDVVVVGAGVAGLVAARQLGRRGLRVALIEARDRIGGRI
                     LTERLSGWPQPVELGAEFVHGGNPILERLLREARVKKRAVEERHWLVRDGRRERVDDA
                     WERIDAVMRKIGPRFRGSFADWLRKHGASIAPEDRELAVTFVKGFQGAPPTRMSARTL
                     FNAAQEEDEQARPAGGYGRVVETLRAGLNGRRVDVRLNTSVTRIDWTRGGATVTASGE
                     RWRARAVLVTVPLGVWRSGAIRFAPALAAKRKILGEIESGHALRVV"
BASE COUNT          260 a          397 c          589 g          280 t
ORIGIN      
        1 ggacggcggg ttccacggcg gaagattttg gcggatcgaa tcgggaggca accatgacgg
       61 atcgaggatg cgtgaacctt aacaggcgcg aaggcgcgcg tcgtccggct gcgttcggtg
      121 caatttttcg agcgtgcggg ctacaggagt cacctacgag gaggccgggc tctgtcccca
      181 tggcgaaata acctgcggtg cgtatgttcc gctgtgccgg gatgaaccgt cacaaccaac
      241 ccttcctttt accatgaatc tcatcgaaac catgctacgc agtcatccgc gcgccgacct
      301 gcagcgggtg gaaaaatacg cggatgtgct caacgcgatc tccttgtgca gccagatttg
      361 cacgtcgtgc gccgacgcgt gtctcgccga ggataacagc gcgcatctga ggcgttgcat
      421 cagcacggat atggattgcg cggatgtgtg cgacgcgacg gcgcggctcg tgatgcggca
      481 gacggaatcg ccgtatgaac tcgtgcacgc gcagttgcat gcgtgcatcc tcgcgtgcca
      541 gatctgtgcg gacgagtgtg agaaacatgc gtcgatgcac gatcattgtc gcatctgcgc
      601 ggagacgtgc cggcattgtc aggagcgctg caatttcctc cttggcgaaa tttcttcgtc
      661 gggcgtggag gaggagtcga tcgatccgac cgacagtcct tcgttccggc gctgaaacgc
      721 tggagcgaat tgtgcatgtc cgacacggag gttgacgtcg tcgtcgtggg cgcgggcgtg
      781 gccgggctag tggcggcgcg acaactgggt cggcgcggct tgcgtgtggc tttgatcgag
      841 gcgcgcgatc gcatcggcgg gcggattttg accgagcggc tgtcgggctg gccgcagccg
      901 gtggagttgg gcgcggagtt cgtgcacggc ggaaacccga tattggaacg gttgctgcgt
      961 gaggcgcggg tgaagaaacg cgccgtcgag gagcggcact ggctggtgcg agacgggcgg
     1021 cgcgaacgcg tcgacgacgc gtgggagcgc atcgacgcgg tgatgcgcaa gatcgggccg
     1081 cggtttcgcg ggtcgttcgc ggattggttg cggaagcacg gcgcgagcat cgcaccggag
     1141 gatcgggagc tggcggtgac gttcgtcaaa ggatttcaag gtgcgccgcc gacgcggatg
     1201 agcgcgcgga cgttgttcaa cgcggcgcag gaggaggacg agcaggcgcg tccggcgggt
     1261 ggatacgggc gggtcgtgga aaccttgcgc gccggcttga acggccgacg cgttgatgtg
     1321 cggttgaaca cgagtgtgac gcgcatcgat tggacgcgtg ggggcgcgac ggtgaccgcg
     1381 agtggcgagc gatggcgcgc gcgggcggtg ttggtgacgg tgccgttggg cgtgtggcgg
     1441 agcggagcga ttcgttttgc gccggcgttg gcggcaaagc ggaagatttt gggggaaatc
     1501 gagagcggac acgcgttgcg cgtggt
//
LOCUS       DASPLT010000874         1438 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_134099, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000874 DASPLT010000000
VERSION     DASPLT010000874.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1438)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1438)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1438
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_134099"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..194)
                     /locus_tag="VEA63_09105"
     CDS             complement(<1..194)
                     /locus_tag="VEA63_09105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007359944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HYD84200.1"
                     /translation="MNEPRWRVWLGAARPRTLPAAAAPIVAASALAWRDGGFDARAAT
                     LCLGFALLVQIGTNFANDYY"
     gene            complement(191..886)
                     /locus_tag="VEA63_09110"
     CDS             complement(191..886)
                     /locus_tag="VEA63_09110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD84201.1"
                     /translation="MTIDWLNLLLAVVLLWLPRQWLRLGSNVIELPRRSRRRSHRDTN
                     PAIARDASDASVSFRAEIGKLRNFVDFFRAALGGVLLLGTLPGREPAFHVVEGAAMSA
                     VNLAANAPLAILLVGVLLQTFRFETRVTLFPPIFYLSGLTFGLCGIYPAVFAMVLVWA
                     VNFALPTPAGFLAVYALLVTTFGLLFQRLDNKLPFVAAGLLFLPVLLSLLTRRRLVLL
                     TKRVKASSSGLAT"
     gene            complement(997..1272)
                     /locus_tag="VEA63_09115"
     CDS             complement(997..1272)
                     /locus_tag="VEA63_09115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007360338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HYD84202.1"
                     /translation="MAEVHHEIVYFSGRVQGVGFRYTTLQIAREFEVAGYVRNLVDGR
                     VQVEAEGRATEVEAFIAAIEERMHGYVRRTERSGQKRAPEFSGFTIR"
BASE COUNT          302 a          516 c          417 g          203 t
ORIGIN      
        1 tcatagtagt cgttcgcgaa gttcgtgccg atttgcacca gcagcgcgaa ccccaggcaa
       61 agcgtcgcgg cgcgtgcatc aaatccgccg tcgcgccacg ccagcgccga cgccgccacg
      121 attggcgccg ccgccgccgg cagcgtgcgc ggacgcgccg ccccgagcca cacccgccat
      181 ctcggctcgt tcacgtcgcc agccccgaac tcgaggcttt cacccgcttc gtcaacagca
      241 ccagccgccg cctcgtcaac aaactcagca gcaccggcaa aaacagcaga ccggccgcga
      301 cgaagggcag tttgttgtcc aagcgttgaa agagcagccc gaatgtcgtg acgagcagcg
      361 cgtaaaccgc gaggaagccc gcgggcgtcg gcagcgcgaa gttcaccgcc cagacgagca
      421 ccatcgcgaa caccgccgga taaatgccgc acagcccaaa cgtgagcccc gacagataaa
      481 agatcggcgg aaacaaagtg acccgggtct cgaaccgaaa cgtttggagc aacacgccca
      541 ccagcaggat cgccagcggc gcgttcgccg cgagattcac cgcgctcatc gccgcgccct
      601 cgaccacgtg aaacgccggt tcgcggccgg gcaacgtgcc gagcagcaat acgccgccga
      661 gcgccgcgcg gaaaaaatcg acgaagttac gcagctttcc gatctcggcc cgaaaactca
      721 ccgacgcgtc gctggcgtcg cgcgcgatcg cagggttggt gtcacggtga gatcgccgcc
      781 gcgaacgccg cggcagttcg atcacgttgc tgccgagacg aagccattga cggggcaacc
      841 aaagcaacac caccgccaga agaagattaa gccaatcgat agtcacgcga gaggcgcagc
      901 gaagaagctc atctcgattg tgtcaaaggt gcagcgcagc ataagcgctt gggcgcgcgc
      961 cttgcgccac ggatcaacgc gggcagacgc accccttcat cgaatcgtga aaccgctgaa
     1021 ttccggcgcg cgcttctgtc cgctgcgctc cgtgcggcgc acgtagccgt gcatgcgttc
     1081 ctcgatcgcc gcgataaacg cctccacttc cgtcgcgcgg ccttccgctt ccacttgcac
     1141 gcgaccgtcc acgaggttgc gcacgtagcc cgccacctcg aactcgcgcg cgatctgcag
     1201 cgtcgtgtag cgaaaaccca cgccttgcac gcggcccgag aaataaacga tttcgtgatg
     1261 cacctccgcc atcgggcgcc aagcgaacaa cggtccgccg aacgcttcaa cccgcaagtg
     1321 cgtcacgccg tgcttcccgc cgctcgccag tcctccgctg tcgaaagcgc tttgcgtcgc
     1381 atcggtgcac gctcacaccc gcgaggcaag gtctgctgcg atcgcatgag cttggagc
//
LOCUS       DASPLT010000875         1377 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_50349, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000875 DASPLT010000000
VERSION     DASPLT010000875.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1377)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1377)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1377
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_50349"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(264..>1377)
                     /locus_tag="VEA63_09120"
     CDS             complement(264..>1377)
                     /locus_tag="VEA63_09120"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HYD84203.1"
                     /translation="ADASVETQLYYDRLTRNESLRSKSTTETIDFTLEHRFAPSERQD
                     LMWGVGYRFHESDVWKTAIAVNTNHYDLKLFSAFVQDEVELVPDALAVTAGVKLEHND
                     FTGAEVQPSVRAMWRASDAQTVWAAVGRSVRTPSALEGKDLFHMEIGPPSPGPDGGLY
                     IPTVLGNAHPDSEVLWSYELGWRMQVSPRVSVDLATFYNRYDELMTFSAMPRFVPGAP
                     FGVAEISFVNGGATETFGGEVAVNVAPTKAWRISASYSALEVRADGAIAGEQYDPQRQ
                     AVLRSSHELGRRVSVDFQLRYVGGFAAPAFPIATAIPSYVGADVRIAYRPNDVLEISV
                     VGRDLLDGQHPEQGPAIYSITSEVPRRVYGKVTWRF"
BASE COUNT          262 a          494 c          381 g          240 t
ORIGIN      
        1 ggcccgcaca ccgcaatcgt gattgcctcc gtgctggttt cgaaatgggc cgcacgccat
       61 tcgacgaact tcgtgaaatt ataaagaaac gccgccttga tttcgtattc cttcgaaaca
      121 tcccgcgacg cgcccaacgc ctgcaatgcg cccgccgcca cccacgcgag agccagcatc
      181 ccgagcctcc acgcacgtcc cgccgacctc cgtttatggg ggacggtgca gcgcgcgaac
      241 gtttgtatcc aaaattccat tcctcagaac cgccaggtga ccttgccgta aacgcgacgc
      301 ggcacttccg atgtgatcga gtagatcgcg ggcccttgtt cgggatgctg gccgtccaac
      361 agatcgcgcc cgacgaccga aatctccagc acgtcgttcg gacgatacgc gatacgcaca
      421 tccgctccca cgtaagatgg aatcgccgtc gcgatcggaa acgccggcgc cgcgaacccg
      481 ccgacgtaac gcaactgaaa gtcgacgctc acgcgccggc ccagttcgtg cgacgagcgc
      541 agcaccgcct ggcgttgcgg atcatattgt tcgccggcga tcgccccatc cgcgcgcacc
      601 tcgagggccg agtagctcgc gctgatccgc cacgccttcg tcggcgccac gttgaccgca
      661 acttcgccgc caaacgtctc cgtcgctccg ccgttcacga acgaaatttc cgcaacgccg
      721 aagggcgcgc ccggcacgaa ccgcggcatc gcgctaaacg tcatcagttc gtcgtagcga
      781 ttatagaacg tcgccagatc gacactcacg cgcgggctca cctgcatgcg ccagccgagc
      841 tcataactcc acaacacctc cgagtcagga tgggcgttgc cgagcaccgt cgggatgtag
      901 agcccgccat ccggccccgg cgaaggcggt ccgatctcca tatgaaaaag atccttcccc
      961 tcgagggcgc tgggcgtgcg caccgaacgc cccaccgcag cccacacggt ctgcgcgtcc
     1021 gacgcccgcc acatcgcgcg cacacttggc tgcacctccg caccggtgaa atcgttgtgc
     1081 tcgagcttca cgcccgcggt gacggcgagc gcgtccggca cgagttccac ttcgtcctgc
     1141 acgaacgcgc tgaacaactt cagatcgtag tgattcgtgt tcaccgcgat cgccgttttc
     1201 cagacatcgc tctcgtggaa ccgatagccg acgccccaca tcaggtcttg tcgttcgctc
     1261 ggcgcgaagc ggtgctcgag cgtgaaatcg atcgtctcgg tcgtcgactt cgagcgcagg
     1321 ctctcgttgc gcgtcagccg gtcgtagtaa agctgcgtct cgacactcgc atcggcg
//
LOCUS       DASPLT010000876         1123 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_153959, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000876 DASPLT010000000
VERSION     DASPLT010000876.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1123)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1123)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1123
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_153959"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(27..671)
                     /locus_tag="VEA63_09125"
     CDS             complement(27..671)
                     /locus_tag="VEA63_09125"
                     /inference="COORDINATES: protein motif:HMM:NF012383.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase"
                     /protein_id="HYD84204.1"
                     /translation="MNRYRDRAEAGHALARLLVGEHRADHAIVLALPRGGVPVAREIA
                     PALDASLDVFVVRKLGLPQHPEVAMGAIASGGIRLLDESLISSERVAPLDVDRVIARE
                     TAELQRRETLYRGNRSAPAIAGRRVILVDDGVATGYTMRVAILALQALRPSRLTVAIP
                     VGAPDTCAELAAAVDELVCPLQPDPFHAVGLWYDHFPPVSDDEVRASLRSAATM"
BASE COUNT          218 a          292 c          425 g          188 t
ORIGIN      
        1 gagcaggcag gcgcggaggc ctgccgctac attgtcgcgg cggagcggag gctggcgcgg
       61 acttcgtcgt cgctgaccgg tggaaaatga tcataccaca agccgacggc gtggaacgga
      121 tcgggctgca acgggcagac gagttcgtcg acggccgcag cgagttcggc gcaggtgtcg
      181 ggcgcgccga cgggaatcgc gacggtaagc cgcgacgggc ggagggcttg tagtgctaaa
      241 atggcgacgc gcatcgtgta accggttgcg acgccatcat cgacaaggat cacgcggcgg
      301 ccggcgatgg cgggggccga gcggttgccc cgataaagcg tttcacgacg ctggagctcg
      361 gcggtttcac gagcgatgac gcggtcgacg tcgagcggcg caactcgttc actggagatg
      421 agcgattcat cgaggaggcg aatgccgccg gaggcgatcg cgcccatggc aacttcggga
      481 tgttgaggca gaccgagctt gcgcacgacg aacacgtcga gcgatgcatc gagagcgggc
      541 gcgatttcac gagcgacggg cacgccgccg cgcgggagtg cgagaacgat ggcgtggtcg
      601 gcacggtgtt caccgacgag gaggcgcgca agggcatgcc cggcttcagc gcgatcgcga
      661 tagcggttca cgggtgtcat gaggccccaa catactgcgg tagaattcac gcgccatggg
      721 agaaggcagt attttggtgg acggtgcggc gggattggtg ctggccgggg tttgtgttgg
      781 agaaggtggc ggatggaggt tgtgaaagag ggttcgaagg aggcgtgaac aaacccggcg
      841 ggacgtcggg ttccacctcg aagagcggaa aaagggggtt acgaaaatca cccatccggg
      901 ccccctcgat tggcctgatt tctttctgca ccttccgggt tacgttgcgg gcgatgaaaa
      961 caatccacat cgcggagatg atcgcgcaag gcggtcggga cggaaaagtc gaggcggccg
     1021 acggcggctt ctcggtcaat ttgtcgtcgg acgagcaatc gggcagcgtg acaccggagc
     1081 atctttttgc cggcgcgtat gcggcgtgtt ttcttggggc att
//
LOCUS       DASPLT010000877         3361 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_50360, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000877 DASPLT010000000
VERSION     DASPLT010000877.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 3361)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3361)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3361
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_50360"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1908)
                     /locus_tag="VEA63_09130"
     CDS             complement(<1..1908)
                     /locus_tag="VEA63_09130"
                     /inference="COORDINATES: protein motif:HMM:NF024627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HYD84205.1"
                     /translation="MPFLRFLLCFSSLAGMAQLAVAAATTTFYVAPDGNDEWSGRLSS
                     RNAAGTDGPLATLERAQDKVRSASKRKGVTVVLRGGVYPRTGSLRLGRKDSGTRNHPV
                     VWRGADGEKPTLSGGVTLFRFERVDDAEVRARITPAARDHVVWTDLRRQGLTDYGTIE
                     QRGSPGLEVFYRGQRQPLARYPNGGWLRIAGVPQTGAERLNEGLEREKRFDGVPAGRH
                     YGRIAYAEDRPNHWAPDPNIFAHGYWVWDWSDSFQQVSLLDPARHELTFAAPHHHYGY
                     AQNQRYYFLNVLEELDEPGEWYLDRQAGRFYFYPPAPLAEGDVQVSMLNQPFIVIEGA
                     HDIAVEELAFTASRGSGVVMRNAQRCRVSGGAFTNLGDVAADISGGEANEISSSDFHD
                     LARGAINVSGGDRRTLRPSRHRVINNHIHHFSQWLRTGQYGIFIDGVGQRVAQNLIHD
                     APFEAMYLRGNDHVIELNEIHSVTQETGDAGGIHTGRDWTWRGHVIRHNYWHHLKGPG
                     VHGVTAVYLDDFSSGFSVTGNIFYRAGRAVQIGGGRDNLVANNLFVECEPAVHVDARG
                     LSWAVNYFDGRFPWMFERFREMKADRPPYATRYPALKTILRDDPAMPKGNRIVHNISW
                     GGRWLDVYDYRV"
     gene            2340..2693
                     /locus_tag="VEA63_09135"
     CDS             2340..2693
                     /locus_tag="VEA63_09135"
                     /inference="COORDINATES: protein motif:HMM:TIGR03433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HYD84206.1"
                     /translation="MSKEKLDLLQGTLDLLVLQTLATMGPMHGYGIARRIEQVGDDEL
                     MLNQGTIYAALVRLQQGKWIDAEWGVSDSNRKAKYYAITARGRKRLAAETENWQRVSG
                     VVHRILSATDASPAS"
     gene            2690..>3361
                     /locus_tag="VEA63_09140"
     CDS             2690..>3361
                     /locus_tag="VEA63_09140"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease prefix domain 1-containing protein"
                     /protein_id="HYD84207.1"
                     /translation="MNAWMDRFVARVRAVFTKRTLDADFADELAHHLELLTADNIKAG
                     LAPDEARRQAQIALGGVEQARELHRETRGLPLFEQFLHDLGYAVRVIRRERGFAIVAL
                     LILAIGVGLNTTVFSLVNTVLLRPMPFAHAERLVWIYNGDPASANRDLSNIASRIDTW
                     EGLQETSRTLEQIEAYNPFSVRQTYRLTGNGDPETILLVNVSHGLFGMLGVNPMLGRQ
                     FAPEDA"
BASE COUNT          652 a         1027 c         1067 g          615 t
ORIGIN      
        1 gacccggtag tcgtaaacgt ccagccagcg accgccccac gaaatgttgt gaacgatacg
       61 attccctttc ggcatcgccg gatcgtcgcg cagaatcgtt ttcagggcgg gatagcgagt
      121 ggcgtaaggc gggcgatccg ccttcatctc gcggaaacgt tcgaacatcc acgggaaccg
      181 gccatcgaaa taattcacgg cccagctcag accgcgcgcg tcgacgtgca cggccggctc
      241 gcattcgacg aaaaggttgt tcgcaaccag gttgtcgcga ccgccgccga tctgaacggc
      301 gcgaccggcg cgataaaaaa tatttcccgt cacgctgaac ccgctcgaaa aatcgtccag
      361 atagaccgcg gtgacgccgt ggacgcccgg tcctttgaga tgatgccagt agttgtgccg
      421 gatgacatgg ccgcgccacg tccagtcgcg tccggtgtga atgccgcccg cgtcgccggt
      481 ttcctgcgtc acgctgtgaa tctcgttaag ctcgatcaca tggtcattgc ctctcaaata
      541 catcgcttcg aaaggtgcat cgtggatgag gttttgggcg acgcgctggc cgacgccgtc
      601 gataaaaatg ccatactggc cagtgcgcag ccactggctg aagtggtgaa tgtgattgtt
      661 gatcacgcga tgacgcgatg gccggagcgt gcgacgatcg ccgccgctga cgttgatcgc
      721 gccgcgggca agatcgtgaa aatcgctgct cgatatttcg ttggcttcgc cgccggaaat
      781 gtccgctgcc acgtcgccca aattcgtgaa cgcgccgccc gagacgcggc agcgctgcgc
      841 gttgcgcatc acgacgccgc ttccgcgact ggcggtaaag gcgagctcct cgacggcgat
      901 atcgtgcgcg ccttcgatca cgatgaacgg ctggttcaac atcgagactt gcacgtcgcc
      961 ctccgccaaa ggcgcgggag gataaaaata aaagcgtccc gcctgccggt cgagatacca
     1021 ttcgccgggc tcgtcgagct cctcgaggac gtttaagaaa taatagcgtt gattttgcgc
     1081 gtatccgtaa tgatgatgcg gcgctgcaaa cgtcagctcg tggcgggcgg gatcgagcaa
     1141 cgagacctgc tgaaaagaat cgctccagtc ccacacccag taaccgtggg cgaaaatatt
     1201 cgggtcgggc gcccagtggt tcggccggtc ctcggcgtag gcgatccggc cataatggcg
     1261 gcccgcgggc acgccatcga agcgcttctc tcgctcgagg ccttcgttga ggcgctccgc
     1321 acccgtttgt ggcacgccgg cgatgcgcag ccagccgcca ttgggataac gcgcgagcgg
     1381 ctgacgctgg ccgcggtaga aaacctcgag cccgggcgag cctcgctgct cgatcgttcc
     1441 atagtcggtc aatccctggc gacgcagatc ggtccaaacc acatggtctc gggccgctgg
     1501 cgtgatgcgc gcccgcacct cggcatcgtc gacgcgttcg aagcgaaaca gcgtcacacc
     1561 gccggacagc gttggctttt cgccgtccgc tccccgccaa acgacgggat ggtttcgcgt
     1621 gccggaatcc tttcgcccga gccggagcga tccggttctt ggatacacgc cgcctcgcaa
     1681 cacgaccgtg acgcccttcc gcttgctggc ggagcgcacc ttgtcctggg ctcgttcgag
     1741 cgtggccagc gggccgtcgg tgccggctgc attgcgagaa gacaagcggc cggaccattc
     1801 gtcgttgccg tcgggcgcga catagaaggt ggtggtcgca gcggcgacag cgagctgggc
     1861 catgccagcg agcgacgaga agcacaggag aaagcgaaga aagggcatgg gggatttggt
     1921 tcgagcggtt gattccgatg aacgattgga gtgcgcgcag cggagagagg gacgcggcac
     1981 cgcgatcgag aaggcggagc gagtcgcgtt gcgcgcaacg tcaaatcgac gctgcaccca
     2041 gagaacacca aacaactccc atttcttaca tcgccgtttc catttcgtcg cgcgcggggc
     2101 ggttgcctgg attacgcaga tacgcgtgca tcgcgggatc gagctccggc agcggtggcg
     2161 cgtgatgttc ttctcgggct gagcggggtt cgctcgcaac agaacctgac gaggacgtcg
     2221 ggttccacct tttggctgca gagcgcggct tcgctcgtgt taaccaagcg tgtttggcat
     2281 ctcctgttga caggccacag gaagtaggtc ccgttctgtg gccaagccac aggaacccca
     2341 tgtcgaaaga aaaactcgat ctactccagg gcacgctcga tttactcgtg ctccaaacgc
     2401 tcgccacgat gggaccgatg cacggttacg gcatcgcacg ccggatcgaa caggtcggcg
     2461 acgatgagct catgctcaat caaggcacca tttacgccgc gctggtccgc ctccaacagg
     2521 gcaaatggat cgatgcggaa tggggcgtat ccgacagcaa ccgcaaagct aaatactacg
     2581 cgatcacggc gcggggccgc aaacgcctgg ccgcggagac cgagaactgg caacgcgtgt
     2641 cgggtgtcgt gcaccgcatc ctgagcgcga ccgacgcgtc gcccgcgtca tgaacgcctg
     2701 gatggatcgg ttcgtcgcgc gagttcgtgc tgtgttcacg aagcgaacgc tcgacgccga
     2761 cttcgccgac gagctggcgc atcacctcga gttgctgacg gcggacaaca tcaaggcggg
     2821 cctcgcgccc gacgaagcgc ggcggcaggc gcagatagcg cttggcggag tcgagcaggc
     2881 gcgcgagttg caccgggaaa cgcgcgggct gccgctgttc gaacagtttc ttcacgatct
     2941 cggatatgcg gttcgggtta ttcggcgcga gcgcgggttt gcgatcgtgg cattgctgat
     3001 cctcgcgatc ggcgtcgggc tcaacacgac ggtgttcagt ctggtgaaca ccgtgctgct
     3061 acgaccgatg cctttcgcgc acgcggagcg gctcgtttgg atttacaatg gcgatccggc
     3121 ttccgcgaac cgcgatctct ccaacattgc ctcgcgaatc gacacctggg aagggctgca
     3181 ggaaacgagt cgcacgctcg aacagatcga agcctacaat ccgttttcgg tgcggcagac
     3241 ttaccggctc acgggcaacg gcgatccgga aacgattctc ttggtcaacg tttcgcacgg
     3301 cctcttcggc atgctcggag taaatccgat gctcgggcgg cagtttgcgc ccgaagatgc
     3361 g
//
LOCUS       DASPLT010000878         3664 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_134121, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000878 DASPLT010000000
VERSION     DASPLT010000878.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 3664)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3664)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3664
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_134121"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1215)
                     /locus_tag="VEA63_09145"
     CDS             complement(<1..1215)
                     /locus_tag="VEA63_09145"
                     /inference="COORDINATES: protein motif:HMM:NF025861.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 97 N-terminal
                     domain-containing protein"
                     /protein_id="HYD84208.1"
                     /translation="MNPRTSSPPPPTTGTQCGRGVALARRLVSCFVVLTVVASASIAA
                     TAPIDLFSPDGSVRVRVSTDAAAHLVYSVAEYDRVKLEPARAGVIVDGVDLGADVELG
                     AMVTRKIDETFSWRGNKRTAANLCQSAEIAVRSRATNREWTLEVRVFDDGAAFRYRVP
                     GAGMRRVSGESTAWQLPRTAYVWFQTHTEDYEGVYQASRADEIPREHLVDKLPRETHL
                     GPPVTVQFDDGSYGLLTEASLYRYSGLTFRPAGDAKLLAAFVHDSDGWQHEGPLLSPW
                     RVVVLSQDLDGLVNSDVIPALGEPPDPELFPDGIETSWIRPGKAPCTWMVFGNDGAQW
                     HRQKQFVDQAAAMGCEYLLVDAGWRSEQWGWLKDGGDVWARAAELCDYAAARGVGIFL
                     WHAYPDGRDDSPG"
     gene            complement(1241..1792)
                     /locus_tag="VEA63_09150"
     CDS             complement(1241..1792)
                     /locus_tag="VEA63_09150"
                     /inference="COORDINATES: protein motif:HMM:NF014069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HYD84209.1"
                     /translation="MSLPTRDEARQLLERHVTDEYQRYHASMVATALEGYADLFREDR
                     DLWFVTGLLHDIDFERHPDTHPRESLRWFAEWNYPEALIHAVEAHAYGYHGYATLPRT
                     KLAAALLATDELCGIFYAYRKLNPVRYGEMKPASIKKKIKDPAFAAKVDRATIYTGCE
                     HLGLSVDEHVANVIRFLANFDEA"
     gene            1945..2838
                     /locus_tag="VEA63_09155"
     CDS             1945..2838
                     /locus_tag="VEA63_09155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HYD84210.1"
                     /translation="MSTSSPSLKPDAAQSGEFALAKDCKVRRLGFGAMRVTGPGVWGD
                     PKDPAAARRLLQRVVELGINFIDTADSYGPEVSERLIAEALHPYPAGLVIATKGGLTR
                     QGPERWAPVGRPEYIQQCVEMSLRRLRVDCIELYQLHRIDPKVPAEETLGVLKELQQA
                     GKIRHVGLSEVSVEEIERAKRILPIVSVQNLYNLGNRQSEAVLQYCEENRIGFIPWFP
                     IAAGELAKAGGPLDQAAKRHGATVGQLALAWLLQRSPVMLPIPGTSSMAHLEENTAAA
                     GLRLDPAEWEEIERASRTSTT"
     gene            2915..3538
                     /locus_tag="VEA63_09160"
     CDS             2915..3538
                     /locus_tag="VEA63_09160"
                     /inference="COORDINATES: protein motif:HMM:NF024949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HYD84211.1"
                     /translation="MKETGRGRAESNINARIAERVRTMRASAGLSLDALAAKSNVSRS
                     MLSLVERGETSATAVVLEKIAKALGAPLALLFEDAATPVSPLSRAQDRTPWRDPESGY
                     VRCNISPANFPSPIKIVEVRLPAGAKVAYEASAPELGFHQQVWVREGTIELTTGAAAH
                     RLATGDCLAMRLDAPTSFRNRTRKAARYVVVVAMMERSRARSGRRGA"
BASE COUNT          685 a         1083 c         1240 g          656 t
ORIGIN      
        1 gccggggctg tcgtcgcggc cgtctggata cgcgtgccag agaaaaatcc cgacgccgcg
       61 agcggcggca tagtcgcaca actcggctgc gcgcgcccag acgtcgccgc cgtcctttag
      121 ccagccccat tgttcgctgc gccacccggc gtcgacgagc aggtattcgc aacccatcgc
      181 ggccgcttga tcgacgaatt gcttttgccg gtgccattgc gcgccgtcgt tgccgaacac
      241 catccacgtg caaggagctt tgccgggccg tatccacgac gtttcgattc cgtccggaaa
      301 aagctcgggg tcaggcggct cgccgagcgc gggaatcaca tcgctgttca cgagtccgtc
      361 gaggtcctgg ctgagaacga caacgcgcca cggagaaagg agcggccctt catgctgcca
      421 gccgtcggaa tcatggacga acgcggcgag cagcttcgcg tcgccggctg gtcgaaacgt
      481 gagtccgctg taacgataca ggctcgcctc cgttagaagt ccgtagctgc cgtcgtcgaa
      541 ttgcacggtg acgggcggac caagatgtgt ttcgcgcggg agcttgtcca cgaggtgctc
      601 ccgcgggatt tcgtcggcgc ggctggcttg gtaaacacct tcgtagtctt cggtgtgagt
      661 ttgaaaccat acgtaggccg tgcgcggaag ctgccacgcg gtcgattctc cggacacgcg
      721 gcgcattccc gcgccgggaa cacgatagcg aaatgccgct ccgtcatcga agacacgcac
      781 ctcgagcgtc cactcgcggt tcgtcgctcg cgaacgcacg gcgatctcgg cgctctggca
      841 gaggttcgcc gcggtgcgtt tgttgcctct ccaggaaaac gtctcgtcga tttttcgcgt
      901 caccatcgcg ccgagctcga cgtcggcgcc gaggtccacg ccgtccacaa tcacgccggc
      961 gcgggctggc tcgagtttga cgcggtcgta ttcggcaacg gaatacacaa gatgcgcggc
     1021 ggcatcggtg gatacgcgca cacgaacgct gccatcgggg ctgaacagat cgatgggggc
     1081 ggtagccgcg atggacgccg atgcgacaac cgtcaggaca acgaaacaag aaacgagtcg
     1141 ccgcgcgagc gcgacgccgc ggccacactg agtgccggtg gtgggaggcg gtggggagga
     1201 agttcgtggg ttcataactg acctcgggaa tggggcaatt ttacgcctca tcgaaattgg
     1261 cgaggaatcg gatcacgttc gccacatgct cgtcgacgga gaggccgagg tgctcgcatc
     1321 cggtgtagat cgtcgcgcgg tcgaccttcg cggcgaacgc cggatccttg atctttttct
     1381 tgatcgaagc gggcttcatc tcgccgtagc gcacgggatt gagttttcgg taagcataga
     1441 aaatcccgca aagctcgtcc gttgcgagca acgcggcggc gagtttcgtg cggggcaggg
     1501 tggcgtaacc gtgataaccg taagcatgcg cctccaccgc gtgaatcagc gcttcgggat
     1561 aattccattc ggcgaaccag cgcagcgact cgcgcggatg cgtgtcgggg tgccgttcaa
     1621 aatcgatgtc atgcaggagg cccgtgacaa accatagatc gcgatcttcg cgaaaaaggt
     1681 ccgcgtaacc ttcgagcgcg gtcgctacca tgctggcatg gtagcgttga tactcgtcgg
     1741 tcacatgcct ttcgaggagc tggcgggcct cgtctcgagt tggcagactc atggcggaag
     1801 cgtgacgcag gcgtcgcgcg cggtgaagcc acaactgcga acgggcttca ctgatccgcc
     1861 atcggtggct ttccgatagc ggaaagggag gacgatgtga gcgcgcgcgt tgcgctttga
     1921 acgttggccg cctaattccc ggccatgagc acttcgtcgc cttcattgaa accagatgcc
     1981 gcgcaaagcg gtgagttcgc cctcgcgaaa gactgcaagg ttcgccggct gggcttcggc
     2041 gccatgaggg tcaccggtcc cggcgtgtgg ggcgatccga aggatccggc ggcggcgcgg
     2101 cggttgctcc agcgcgtcgt cgagctcggc atcaacttta tcgatacggc ggattcttac
     2161 ggcccggaag tcagcgaacg cttgattgcc gaggcgctgc atccgtatcc cgcgggactg
     2221 gtgattgcga cgaaaggcgg cctgacgcgt cagggtccgg agcggtgggc gcccgtcggc
     2281 cgtccggaat atattcaaca atgcgtggag atgagcctgc gccggctgcg ggtggattgc
     2341 atcgaacttt accaactaca ccggatcgat ccgaaggtgc ccgccgagga aacgctgggt
     2401 gtgctcaagg agctgcaaca agcggggaag attcgccacg ttggcctttc ggaagtttcc
     2461 gtggaagaga tcgaacgggc aaagcgaatc ctgccgatcg tgagcgtgca gaatctctac
     2521 aatcttggaa accgccagag cgaggcggtg ctgcaatatt gcgaagaaaa ccggatcggc
     2581 ttcatcccct ggtttccgat cgccgcgggc gagctggcga aggcgggcgg tccgctcgat
     2641 caagcggcga agcggcacgg cgcgacggtc ggccagctcg ccctcgcatg gctgttgcaa
     2701 cgctcgcccg tgatgctgcc gattccaggc acgtcttcga tggcgcatct cgaggaaaac
     2761 acggcggccg ccggactcag gctcgatccg gcggagtggg aggaaatcga acgggcgagc
     2821 cggacctcca cgacgtgacg cggggcgagg acgccgcgat gctgccgcgg aagcagcatt
     2881 gacgctttcc ggtttgctgg acagcgtgtc cagtatgaaa gagaccggcc gagggcgcgc
     2941 tgaatcgaac atcaacgcac gcatcgcgga gcgtgtgcgc acgatgcgag caagcgcggg
     3001 cttgagcctc gatgcgctcg cggcgaaatc gaacgtcagc cgttccatgc tgtcgctcgt
     3061 ggagcgcggc gaaacgagtg cgacggcggt cgtcttggag aagattgcga aagcgctggg
     3121 cgcgccgctg gcgctgttgt tcgaagacgc cgcgactccg gtcagcccgc tttcgcgcgc
     3181 gcaggatcgc acgccgtggc gcgatccgga atcgggctat gtgcgctgca acatttcgcc
     3241 ggcaaacttt ccgtctccca tcaaaatcgt ggaagtcaga ttacccgcgg gcgcaaaagt
     3301 ggcttacgaa gccagcgcgc cggagctggg ttttcatcaa caggtgtggg tgcgcgaagg
     3361 cacgatcgaa ctgacaaccg gcgccgcggc gcaccggctg gcgacgggcg actgcctcgc
     3421 gatgcgactg gatgcgccga caagttttcg aaaccggacg cgcaaggcgg cgcgctatgt
     3481 cgtcgtcgtt gcgatgatgg aacgatcgcg tgcgcgttcc gggagacgcg gggcatgacg
     3541 ggcagcttcc aaattcggat tctgcaatcg ttcagcccgc gggagattgc cggactgagc
     3601 gaggtcttga tcgactgcgt ggagggcggg gcgtcggtga gtttcatgct gccgatgaca
     3661 cggg
//
LOCUS       DASPLT010000879         1210 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_50375, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000879 DASPLT010000000
VERSION     DASPLT010000879.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1210)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1210)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1210
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_50375"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            215..>1210
                     /locus_tag="VEA63_09165"
     CDS             215..>1210
                     /locus_tag="VEA63_09165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007359922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HYD84212.1"
                     /translation="MSRILGIDLGTTNSCMAIMEGGEPVVIPNAEGARTTPSVVAFTK
                     TGERLVGQAAKRQAVTNPRNTIFSAKRLIGRRFTEVKAEASNMPFNVVEGKNGDAYIE
                     VQVGDKTEQFAPQQIAAFVLGKLKADAEAYLGEKITQAVITVPAYFNDSQRQATKDAG
                     KIAGLEVLRIINEPTAASLAYGLDKKKDEKIAVFDLGGGTFDVSVLEIGDGVFEVKAT
                     NGDTHLGGDNWDEAVINWLVDTFKKDNGIDLRKDPMALQRLKEEAEKAKIALSSTMTV
                     DINLPFITADASGPKHLNVQLTRAKLEQICDSLFQRMIPPFKNCVKDADLTSAQID"
BASE COUNT          268 a          333 c          389 g          220 t
ORIGIN      
        1 tgcgacgggt gctcaagtta ttttcgctgg cgggcggagg attgtgccac gacgtgtcac
       61 acgtttggcg gttgtgccaa gcgcgtgcca aggtggcgcg gatggtgacc tttcgcagcg
      121 gtgtcggaag cgctgttaag attttaagcc gctaaaattg cgcgggttag cgccagcaat
      181 cgaatggggg cacatgcgtt gctaaacgcg aggcatgagc cgaattttag gaatcgatct
      241 aggcaccacg aactcgtgta tggcgatcat ggagggcggc gagccagtgg tgatcccgaa
      301 tgcggagggc gctcgcacca ccccttccgt cgtggccttc accaagaccg gcgaacgtct
      361 cgtcggtcag gccgcgaaac ggcaggcggt caccaatccc cgcaacacca ttttttcagc
      421 gaagcgtctc atcgggcgca ggttcaccga agtgaaggcc gaggcgtcga acatgccgtt
      481 caacgtcgtc gaaggcaaaa acggtgacgc ttacatcgaa gtgcaggtcg gcgacaagac
      541 ggagcagttc gcgccgcagc agatcgcggc gtttgtgctc ggtaagttga aggccgacgc
      601 cgaggcgtat ctcggcgaga agatcacgca ggcggttatc accgtccccg cgtatttcaa
      661 cgactcacag cgtcaggcga ccaaggatgc cggcaaaatc gccggtctcg aggtgctgcg
      721 catcatcaac gaaccgacgg cagcgtcgct cgcttacggt ctcgacaaga agaaggacga
      781 gaagatcgcg gtgttcgatt tgggcggcgg cacgttcgac gtgtcggtgc tcgagatcgg
      841 cgatggcgtg ttcgaggtga aggccacgaa tggcgacacg cacctcggcg gcgacaactg
      901 ggacgaagcg gtcatcaact ggctcgtcga cacgtttaaa aaggacaacg gcatcgatct
      961 gcgcaaagat ccgatggcgc ttcagcgcct gaaggaagaa gcggaaaagg cgaagatcgc
     1021 gttgtcgtcg acgatgacgg tcgatatcaa cctgccgttc atcacggcgg atgcgtccgg
     1081 cccgaagcat ctcaacgtgc agctcacacg cgcgaagctg gagcaaatct gcgattcgct
     1141 ttttcagcgc atgattccgc cgttcaagaa ctgcgtgaag gacgcggatc tcacctcggc
     1201 gcagatcgac
//
LOCUS       DASPLT010000880         1193 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_11172, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000880 DASPLT010000000
VERSION     DASPLT010000880.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1193)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1193)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1193
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_11172"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1052
                     /gene="rnhC"
                     /locus_tag="VEA63_09170"
     CDS             <1..1052
                     /gene="rnhC"
                     /locus_tag="VEA63_09170"
                     /inference="COORDINATES: protein motif:HMM:TIGR00716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HYD84213.1"
                     /translation="FLFCAFGRSLRLCSPMPKKPTEDDAPKKLASYTAKLDDAQMEKL
                     RAICAARGFSPFEVAYTRFAYKSDAARVNVAAYTSGKVVIAGKGTEEFVTMTLEPEVT
                     GAPKLGYDEVLHPDWFEPHAGLDESGKGDFFGPVIAATVIADKAAIEGWRKAGVQDSK
                     KITEQRIIELDKAIRDTKGVVVRTCFCGMPKYNELMSRPGANLNRLLAWQHATALEQA
                     LAVKSVPRGLLDQFTEQPLVQRELAKKGVKDFELSMRTKAEEDPVVAAASVVARAEFV
                     RQMHLLSKKFGAKLQKGAGPLVKQQAHEIIAKFGAQGLKEFAKLHFRTAYEVVSAAGK
                     LDELPLPEPREKMEW"
BASE COUNT          237 a          305 c          445 g          206 t
ORIGIN      
        1 gttttctgtt ttgcgctttc ggccgcagtt tgcggctatg ttcgcccatg ccgaaaaagc
       61 cgaccgagga cgacgcgccg aagaagctcg cgtcctacac cgccaaactc gacgacgctc
      121 aaatggaaaa actgcgcgcg atctgcgcgg cgcgcgggtt ttcgccgttc gaggtggctt
      181 acacgcggtt cgcttacaag tcggacgctg cgcgcgtgaa tgtcgcggct tacacgagcg
      241 gcaaggtcgt gattgcgggc aagggcacgg aggagttcgt gactatgacg ctcgagccgg
      301 aagtcacggg cgcgccgaaa ctcggctacg acgaagtgtt gcaccctgac tggttcgagc
      361 cgcatgcggg cctcgacgaa agcggcaagg gcgatttttt cgggccggtg atcgcggcga
      421 cggtgattgc ggacaaggcg gcgatcgagg gctggcgcaa agccggcgtg caggattcga
      481 agaagatcac ggagcagcgg atcatcgagc tcgacaaggc aatccgcgac acgaagggcg
      541 tggtggtgcg cacgtgtttc tgcgggatgc cgaaatacaa cgagttgatg tcacggccgg
      601 gggcgaattt gaaccggttg ctggcgtggc aacatgcgac ggcgctcgag caggcgctgg
      661 cggtgaagag cgtgccgcgc gggttgctcg atcaatttac ggagcagccg ctggtgcagc
      721 gcgaactggc gaagaaaggc gtgaaggact tcgagctgag catgcgcacg aaggcggaag
      781 aagatccggt cgtcgcggcg gcgtcggtgg tggcgcgggc ggagttcgtg cggcagatgc
      841 acctgttgtc gaaaaaattt ggcgcgaaac tgcagaaagg cgcggggccg ctggtgaagc
      901 agcaggcgca cgagatcatc gcgaagttcg gcgcgcaggg attgaaggag tttgcgaagc
      961 tgcattttcg cacggcgtat gaagtggttt ccgcggcggg gaaactcgac gagttgccgt
     1021 tgccggagcc gcgggagaag atggaatggt gaggatgatc gaaaggatcg acagggtagg
     1081 gacggctctc cgagccgtcc gcggcggacg tcgagtgatt ttcaactttc gcgcgggtgt
     1141 ctggcctgac ggcgtggctc gaagcggacg cctcggagag gcgtccctac cgg
//
LOCUS       DASPLT010000881         1617 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_175049, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000881 DASPLT010000000
VERSION     DASPLT010000881.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1617)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1617)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1617
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_175049"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            10..>1617
                     /locus_tag="VEA63_09175"
     CDS             10..>1617
                     /locus_tag="VEA63_09175"
                     /inference="COORDINATES: protein motif:HMM:NF012686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD84214.1"
                     /translation="MIGKIASHSAGTGDGFFLAGRKLGKIYQFFLNFGNSVAANDAVS
                     TASLVYQQGISGVWAGFQMIFINPYYWFLFPWYRRVRLTTTADIFLDRLGSPRLALFY
                     ATFQIVMAVAVTMGFGNLVAYKISSAIVTKPEATWTAAERGAVHDYRELKQLEAEQRL
                     GTTSSSSSPRLDQLREREARGELSSYITALEPWIFYTSYTLVVGFYIVMGGLAAAALN
                     EALQGVLIIVFSVILIPSGLIAIGGWDGLRTAVPAGMFNLFGTDGGSDFTGWSIAAVF
                     LATLMQAHAMPGNMAIGGSAKNEFAARFGGVAGTFGKRLVTIAWGVCGLIAVALYSGA
                     NSLTDPDAAWGAMSLQLLGPGLLGLMLAGVLAAQMSTISAQAMSVSALFAHNVYKNFR
                     SSATEADLIRVGRWTLVVVLSLGVLVSTQMTSVYTVLQFAMTINVPFGAAILLMFIWR
                     RLTVPAVWTAVIVSALCNILFPLAAQRFSSLSTHPVLTTRAADAGGRPTPVFFERVVR
                     IDPTQGDSALEGHGRLHTELVLLHALGM"
BASE COUNT          254 a          544 c          478 g          341 t
ORIGIN      
        1 accctggtcg tgatcggaaa aattgcctcg cacagcgccg gcaccggcga cggatttttt
       61 ctcgccggac ggaagctcgg gaaaatttat cagttcttcc tcaacttcgg gaattccgtc
      121 gccgcgaacg acgccgtcag cacggcgagc ctcgtctacc agcagggcat ctcgggcgtg
      181 tgggccgggt tccagatgat tttcatcaat ccttattact ggttcctgtt tccgtggtat
      241 cggcgcgttc ggctcaccac gaccgcggat atctttctcg accgactcgg cagcccccgg
      301 ctcgcgctgt tttacgccac ctttcaaatc gtcatggccg tggccgtgac catgggtttc
      361 ggcaatctcg tcgcgtataa aatttcatcg gcgatcgtca ccaagcccga ggccacgtgg
      421 acggccgccg agcgcggtgc cgtgcacgac tatcgcgagt tgaaacagct cgaagcggaa
      481 caacgcctcg gcacgacgtc gtcctcgagc agcccgcgac tcgaccaatt gcgcgagcgc
      541 gaggcgcgtg gtgagttgag cagctacatc accgcgctcg agccgtggat tttctacacc
      601 agttacacgc tcgtcgtcgg attctacatc gtcatgggag gcttggcggc agccgccttg
      661 aacgaagcgc ttcaaggcgt gctcatcatt gtgttttccg tcatcctgat tccgtcggga
      721 ttgatcgcca tcggtggctg ggacgggctg cgcacggccg tgccggcggg gatgttcaac
      781 ctgtttggaa ccgacggcgg ctccgacttc accggctggt ccatcgccgc cgtgtttctc
      841 gccacactga tgcaagcgca tgcgatgccc ggcaacatgg cgatcggcgg ctcggcgaaa
      901 aacgagttcg ccgcgcgctt cggcggcgtt gccggcacct ttgggaaacg cctcgtcacg
      961 atcgcgtggg gcgtctgcgg attgatcgcc gtcgccttgt attccggcgc caattctctc
     1021 accgaccccg acgccgcgtg gggcgcgatg tcgctccaac ttctcggtcc cggcctgctc
     1081 ggactgatgc tcgcgggcgt gctcgcagcg cagatgtcca ccatttccgc gcaagccatg
     1141 agcgtatcgg ccctcttcgc gcacaacgtc tacaaaaatt tccgctccag cgcgacggaa
     1201 gcggatctga ttcgcgtcgg ccgctggacg ctcgtcgtgg tgctgagcct cggcgtgctc
     1261 gtctccaccc agatgacgag cgtctacacc gtcctgcagt tcgccatgac catcaacgtg
     1321 ccgtttggcg cggccattct cctgatgttt atctggcgcc gcctcacggt tcccgcggtg
     1381 tggaccgccg tgatcgtgtc cgcgctttgc aacattctct ttcccctcgc cgcccaacgc
     1441 ttctcctcgc tgtccacgca tcccgtgctg accacccgcg cggcggatgc cggcggccgg
     1501 ccaacgccgg tctttttcga gcgagtcgtg cgcatcgatc cgacccaagg cgacagcgcg
     1561 ctcgaaggcc acggccggct ccacaccgag ttggtgttgc tgcacgcgct cggcatg
//
LOCUS       DASPLT010000882         1892 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_195727, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000882 DASPLT010000000
VERSION     DASPLT010000882.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1892)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1892)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1892
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_195727"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            194..1600
                     /gene="hemN"
                     /locus_tag="VEA63_09180"
     CDS             194..1600
                     /gene="hemN"
                     /locus_tag="VEA63_09180"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HYD84215.1"
                     /translation="MIAPSENFSATSAPLDLDLIRKYSIPGPRYTSYPPATKFTGEIA
                     GQRLIEAIDEDNAVGAGPLSLYFHLPFCESRCWFCGCNTVITRRRESAGEYLDDLERE
                     MRLTAARMDRTRAVTQIHLGGGTPTFFPPDELRRLGGLIREIFPQRPANCEFGVEVDP
                     RRLTAEHVEALREIGANRASLGVQDTNARVQLAIHRVQPQAVNRQSFAWLRAAGFASI
                     NVDLIYGLPLQTPGTFARTINDVLDLNPDRLSVFSYAHVPWIKPAQKIFDDREQLPDA
                     EAKLAMFAIAHEKLTATGYVDIGLDHFARPDDELAIALREGTLHRNFQGYSTRGGASL
                     YSFGISSISATATTYRQNFKTLDAWRAALDASELPVERGLRLSEEDLRRRTIIMRIMC
                     DRKLDFAALSQSLGVDFAGTYAKEIASLADLEADGLVARTRNGVAVTRAGAPLLRVVA
                     MRFDPQLSAGAQEHSRTI"
BASE COUNT          326 a          586 c          628 g          352 t
ORIGIN      
        1 cgcaactgct gatggcattg gagcgcgaac tcgcgaaggc gcgcgattaa cgcggtgtga
       61 gatcgctcgc gatgcacgcg gcgcggcggg cgcgagatca tcgcgcaggt tggcaatgaa
      121 tgcgccattg ctggcaaacg ccgcgcagcc acggcgccga ggttatgcga cactgcgcgc
      181 gcacattccc gtcatgatcg ccccctctga aaatttctcc gcgacgtcag cgccgctcga
      241 cctcgatctc atccgcaagt attcgattcc cggtccgcgc tacacctctt atccgcccgc
      301 gacgaagttc accggcgaga tcgcgggcca gcgcttgatc gaggcgatcg acgaggacaa
      361 cgccgtcggg gcggggccgt tgtcgttgta ttttcacctg ccgttttgcg agtcgcgctg
      421 ctggttctgc ggctgcaaca ccgtcatcac ccgccgccgt gaatccgccg gggaatacct
      481 cgacgatctc gagcgtgaga tgcgtctcac ggcggcgagg atggatcgca cgcgggcggt
      541 gacacaaatt cacctgggcg gcggcacgcc gacatttttt ccgcccgatg agttgcgccg
      601 gctcggcggg ttgattcgcg agatttttcc gcagcgcccg gcgaactgtg aattcggagt
      661 ggaggtggac ccgcggcggt tgaccgccga gcacgttgag gcgttgcgcg aaatcggcgc
      721 caatcgcgcg tcgctcgggg ttcaggacac caatgcgcgg gtgcaactcg cgattcaccg
      781 ggttcaaccg caggccgtca accggcagtc gtttgcctgg ctgcgcgcgg cgggcttcgc
      841 gtcgatcaat gtcgatctga tctacgggct gccattgcaa accccgggaa cgttcgcgcg
      901 gacaatcaac gacgtcctcg atctcaaccc cgaccgcctg tcggttttca gctacgcgca
      961 cgtgccgtgg atcaagccgg cgcagaaaat cttcgacgat cgcgaacagt tgcccgacgc
     1021 ggaggcgaag ctcgcgatgt tcgcgatcgc gcacgaaaag ctcacggcga ccggatacgt
     1081 cgacatcggc ctcgatcatt tcgcgcggcc ggacgacgaa ctcgcgattg cactgcggga
     1141 agggacgctg catcggaatt tccaaggcta cagcacgcgc ggcggagcgt cgttgtattc
     1201 gttcggcatt tcgtcgatct cggcgacggc gacgacgtat cggcagaatt ttaaaacgct
     1261 cgacgcctgg cgcgccgcgc tcgatgccag cgaattgccg gtcgaacgtg ggctgcggtt
     1321 gagcgaggaa gacctgcggc gccgcacgat catcatgcgc atcatgtgcg accggaagct
     1381 ggacttcgcg gcgctctcgc agtcgcttgg cgtcgatttc gccgggacgt atgcgaagga
     1441 gatcgcttcg ctggcggatc tcgaagcgga cggacttgtg gcgcgcacga ggaacggtgt
     1501 cgcggtgacg cgcgcgggcg cgcccctgtt gagggtggtt gcgatgcgtt tcgatccgca
     1561 gttgtcggca ggagcgcagg aacactcgcg cacgatttga ttcggcggtg aaggcgtgct
     1621 tcgcagagcc caatatctgc gcgacgtccg ccaagcggtg cggagcgggc ggcggcgaat
     1681 gcgacgataa tcacctcacg cggcggttcc gcgatggagc tgtcgccctc ctcctatgaa
     1741 aacagcacgc agattcaaag gctcctggtt cctcctgacg atggtcgccg cggtggcgct
     1801 cggcgaagtg atcgcgtggg agatctcgcg tttctacggc gtcgcgcttc acccgatcac
     1861 ggtggcgttt gcctgcatcg gcctggtgca cg
//
LOCUS       DASPLT010000883         1172 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_34354, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000883 DASPLT010000000
VERSION     DASPLT010000883.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1172)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1172)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1172
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_34354"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..542
                     /locus_tag="VEA63_09185"
     CDS             <1..542
                     /locus_tag="VEA63_09185"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HYD84216.1"
                     /translation="ATGATTKGKGPARLRVGDAVPAFALIDQAGKPIDESELKGRHTI
                     VTFIFTRCPVPEFCPLIARKFQALQQTLAEAKAGDDVRLLSISIDPEHDRPPVLREYG
                     EALGADFDRWRFATGTNEQIATLAKLFAVRTERNGGSLDHTLATALIGADGRVVEIWR
                     GNGWKPEEVAAKVREGAAR"
     gene            753..>1172
                     /locus_tag="VEA63_09190"
     CDS             753..>1172
                     /locus_tag="VEA63_09190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HYD84217.1"
                     /translation="MADTQRHPFLHGLSKHRGAAPTVVVIFGASGDLTARKLIPAVYN
                     LALDGLLPADFFLVGYGRKPISDDEFRGLAADAIKEFSRRELQDDVWRRTAAHTSYVA
                     GGYDEKEAFDRLAAHIAELEKKLGREVQTLFYVSTPPS"
BASE COUNT          211 a          338 c          425 g          198 t
ORIGIN      
        1 gcgccacggg cgcgacaacg aagggaaaag ggccggcgcg gttgcgggtc ggtgatgcgg
       61 tgccggcgtt cgcgctgatc gatcaggcgg ggaagccgat cgacgagtcc gagctgaagg
      121 gacgtcacac gattgtgacc ttcattttca cacgctgccc ggtgccggag ttttgtccgc
      181 tgatcgccag gaaatttcag gcgttgcagc aaacgttggc cgaggcgaag gcgggtgacg
      241 acgtgcggct cttgagcatc tcgatcgacc ccgagcatga ccggccgccg gtgttgcgcg
      301 agtatggcga agcgttgggc gcggatttcg atcggtggcg ttttgcaacg ggaacgaacg
      361 agcagattgc gacgctcgcg aaactgttcg cggtgcgcac ggagcggaac ggcggctcgc
      421 tcgatcacac gctggcgacg gcgttgatcg gagcggatgg ccgcgtcgtg gagatctggc
      481 ggggcaacgg ctggaagccg gaagaggtcg cagcgaaggt gcgtgagggg gccgcacggt
      541 aggcgcgagg cgggttatga gtgggcggat cgaatagggg gagggaagcg aggaggggac
      601 caccgtccag gtcgatcgca cgcgttgtgt tgtgacggga ccgtgttgtc cctgcgctca
      661 cgcgcagagc cacgaaccct gacacgctga cacgcatggg cgaactgacg gcttgcgtca
      721 ctttagcgag gctccgaact tcgcgggcac gcatggccga cacacagcgt catcctttcc
      781 tgcacggact cagcaaacac cgcggcgccg ccccgaccgt cgtggtgatt ttcggcgcct
      841 cgggcgatct gaccgcgcgc aaactcattc ccgcggttta caacctcgcg ctcgacggac
      901 tcctgcccgc ggatttcttc ctcgtgggct acggacggaa gccgatttcc gacgacgagt
      961 ttcgcggact tgcggccgat gcgatcaagg agttttcgcg gcgcgaattg caggacgacg
     1021 tctggcgccg caccgctgcg cacacgagct acgtggcggg cggctacgac gagaaggaag
     1081 cgttcgaccg gctcgcggcg cacatcgcgg agctggagaa gaagctcggt cgcgaagtgc
     1141 aaacgttgtt ctacgtttcg acgccgccgt cg
//
LOCUS       DASPLT010000884         1161 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_214698, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000884 DASPLT010000000
VERSION     DASPLT010000884.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1161)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1161)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1161
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_214698"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..847
                     /gene="sufB"
                     /locus_tag="VEA63_09195"
     CDS             <1..847
                     /gene="sufB"
                     /locus_tag="VEA63_09195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007358969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HYD84218.1"
                     /translation="FIYVPPGVKVSHPLQAYFRINAENFGQFERTLIIADEGSEVVYM
                     EGCTAPKFSTSTLHSAVVELVALKGAKIQYITVQNWAPNVFNLVTKRGVAHENAEIKW
                     IDCNIGSRLTMKYPGVVLKGKRARGEVISIALANDGQHQDTGAKMIHAADDTTSNVVS
                     KSISVGQGRATYRGQVHIPKHLKGCKNNTECDALLINTNSRTDTYPAITVRGDKNATQ
                     HEASVSKVSEDMIFYMQQRGLTEGQAMSLAVNGFINDLARQFPMEYSVELKRLIDLEM
                     EGSVG"
     gene            945..>1161
                     /locus_tag="VEA63_09200"
     CDS             945..>1161
                     /locus_tag="VEA63_09200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HYD84219.1"
                     /translation="MSATALSQTTVGSFTPEAFDAHLATQSSAPVWWLDRKRDAYARF
                     AALPMPKRTDESWRFSNISTLTLDGFQV"
BASE COUNT          254 a          420 c          293 g          194 t
ORIGIN      
        1 gttcatctac gtcccgcccg gcgtgaaggt ctcgcacccg ctgcaagcct acttccgcat
       61 caacgccgaa aacttcggcc aattcgaacg caccctcatc atcgccgacg agggcagcga
      121 ggtcgtttac atggaaggct gcaccgcgcc gaagttctcc acctcgacgc tccacagcgc
      181 tgtcgtcgaa ctcgtcgcgc tcaagggcgc gaagatccaa tacatcaccg tccagaattg
      241 ggcgcccaac gtcttcaatc tcgtcaccaa gcgcggcgtc gcccacgaga acgccgagat
      301 caagtggatc gactgcaaca tcggcagccg cctcaccatg aaatatcccg gcgtcgtcct
      361 caaaggaaag cgcgcccgcg gcgaggtcat ctccatcgcc ctcgccaacg atggccagca
      421 ccaggacacc ggcgcgaaaa tgattcacgc cgccgacgat acgacctcca acgtcgtttc
      481 gaaatccatc tccgtcggcc agggccgcgc cacgtatcgc ggacaggttc acatcccgaa
      541 gcacttgaag ggttgcaaaa acaacaccga gtgcgacgcg ctgctcatca acacgaacag
      601 ccgcaccgac acctatccgg cgatcaccgt ccgcggcgac aaaaacgcca cgcagcacga
      661 agcttccgtc tcgaaggttt cggaagacat gatcttctac atgcagcagc gcggtctcac
      721 cgagggccag gcgatgagcc tcgccgtgaa tggtttcatc aacgacctcg cccgccagtt
      781 cccgatggaa tacagcgtcg agctcaagcg cctcatcgac ctcgaaatgg aaggctccgt
      841 cggctgaaaa gctgaacgcg agggacgcga accgcgcaga cccatttctg ctgcgtctct
      901 gcgtcctccg cgctctccgc gtttaagaaa attcatcttc acgaatgtcc gcgaccgccc
      961 tctctcaaac caccgtcggc tccttcaccc cggaggcttt cgacgcccat ctcgcgacgc
     1021 aatcgtccgc gcccgtctgg tggctcgacc gcaaacgcga cgcctatgct cgtttcgccg
     1081 cgctccccat gccgaagcgc accgacgaat catggcgctt cagcaacatc agcaccctca
     1141 cgctcgacgg cttccaggtg g
//
LOCUS       DASPLT010000885         1835 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_175086, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000885 DASPLT010000000
VERSION     DASPLT010000885.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1835)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1835)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1835
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_175086"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..208
                     /locus_tag="VEA63_09205"
     CDS             <1..208
                     /locus_tag="VEA63_09205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="twin-arginine translocation pathway signal
                     protein"
                     /protein_id="HYD84220.1"
                     /translation="TAAPLFVMGSRVNAGVHGTAPSLRLERNQDLAFSTDFRRVYATM
                     LDQWLACPSTAVLGREYGALPLIA"
     gene            280..1218
                     /locus_tag="VEA63_09210"
     CDS             280..1218
                     /locus_tag="VEA63_09210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HYD84221.1"
                     /translation="MQLHGIHHLTAVTAQASRNHAFYTRMLGMRLVKKSVNQDDVSAY
                     HLFYADGAASPGTDLTFFDWPAARENRGTHSIVRTGLRVANEASLKWWAARFAAEAVT
                     QEPMTTRDGRATLDFEDFEGQRFSLVVDSKPNEPARPWAKSPVLAEHQILGLGPVMLS
                     VPKLAPTESVLTSVMNMRRVREYALGAWAVHVYEMGPGGPAAELHVAVQPELSIVRPG
                     AGGVHHVAFRTTVPDYAAWTDRLQSLGVPSSGPVDRYYFKSLYFREPNGILFELATDE
                     PGFAVDEPMESLGEKLALPPFLEGRRTEIEAGLKPL"
     gene            complement(1293..>1835)
                     /locus_tag="VEA63_09215"
     CDS             complement(1293..>1835)
                     /locus_tag="VEA63_09215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007362493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HYD84222.1"
                     /translation="KFVGTGEKTADFEPFYSDRLASRILGMGDVVSLVEKAQETIDQK
                     DAEKMAEKLRKADFNLEDFLSQMQQVKKMGSMQSIMGMIPGMSGMQLPEGADKQMART
                     EAIIKSMTVQERRKPEILNGSRRKRIADGSGTKIVEVNQLLKQFQEMQKMMKMFKGGG
                     NKKMMRQMEAMRSRGGFPGM"
BASE COUNT          302 a          498 c          637 g          398 t
ORIGIN      
        1 gacggcggcg ccgctgttcg tgatgggttc gcgcgtgaac gccggcgttc acggcaccgc
       61 gccgtcgttg aggctcgagc gaaaccagga tctcgcattt tcgacggatt tccggcgcgt
      121 gtatgcgacg atgctcgatc agtggctggc gtgcccgtcg accgcggtgc tggggcggga
      181 gtatggtgcg ctgccgctga tcgcgtgaag ggttggcgtt aagcgatttt cgagcagtcg
      241 tcgctggacg aaacgaaggt ccgggccaca ttgcgggcga tgcaacttca cggaattcac
      301 cacctgaccg cggtcacggc gcaggcttcg cgcaaccacg cgttttatac gcgcatgctc
      361 gggatgcgcc tcgtgaagaa atcggtgaat caggacgacg tatcggcgta tcacttgttc
      421 tacgcggatg gggccgcgtc gccgggaacg gatctgacgt ttttcgactg gccggcggcg
      481 cgggaaaacc gcggaacgca cagcatcgtg cgcacgggac tgcgcgtggc gaacgaggcg
      541 agcttgaaat ggtgggcggc gaggtttgcg gcggaagcgg tgacgcagga accgatgaca
      601 acgcgtgacg ggcgagcgac gctggacttc gaggattttg aaggtcagcg cttcagcctg
      661 gtggttgaca gcaagccgaa cgaaccggcg cggccgtggg cgaagagccc ggtgctggcg
      721 gagcaccaga ttctcggatt ggggccggtc atgttgagcg tgccaaaact cgcgccgacg
      781 gaaagcgtgt tgacgtcggt gatgaacatg cgacgcgtgc gcgagtatgc gctcggtgcg
      841 tgggcggtgc atgtttacga aatgggcccg ggcggtccgg cggcggagtt gcacgtcgcg
      901 gtgcagccgg agttgtcgat cgttcggccc ggcgcgggcg gggtgcatca cgtcgcgttt
      961 cggacgacgg tccccgatta cgcggcgtgg acggatcgtt tgcagagcct cggcgtgccg
     1021 tcgagcgggc cggttgaccg ctattatttc aagagcctgt atttccggga gccaaacgga
     1081 attttgtttg agctcgcgac ggatgagccg ggttttgcgg tggatgaacc gatggaatcg
     1141 ctcggcgaaa aactggcgct gccgccgttt ctagaaggac ggcgaacgga gatcgaggcg
     1201 gggttgaagc cgctgtgacg caccgattcc cgctcctcac ggagcgagcc acgttggttg
     1261 tgtggctctg cgcgtgagcg cagggattcg aattacattc cgggaaagcc gccgcggctg
     1321 cgcatggctt ccatctggcg catcatcttt ttgtttccgc cgcctttgaa cattttcatc
     1381 atcttctgca tttcctgaaa ctgcttcagg agctggttga cctcgacgat cttcgtgccg
     1441 gagccgtcgg cgatgcgttt gcggcggctg ccgttgagga tctcgggttt gcggcgttcc
     1501 tgcacggtca tcgatttgat gatggcttcg gtgcgcgcca tttgtttgtc cgcgccttcg
     1561 ggcagctgca tgccgctcat gccggggatc atgcccatga tgctctgcat cgatcccatt
     1621 tttttcacct gctgcatctg cgagaggaaa tcttcgaggt tgaagtcggc cttgcggagt
     1681 ttctccgcca ttttctccgc gtccttctgg tcgatcgttt cctgcgcttt ttcgacgagc
     1741 gaaacgacgt cgcccatgcc gaggatgcgc gaggcgaggc gatcggaata aaacggttcg
     1801 aagtcggcgg ttttttcgcc ggtgccgacg aattt
//
LOCUS       DASPLT010000886         2249 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_50425, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000886 DASPLT010000000
VERSION     DASPLT010000886.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 2249)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2249)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2249
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_50425"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..738
                     /locus_tag="VEA63_09220"
     CDS             <1..738
                     /locus_tag="VEA63_09220"
                     /inference="COORDINATES: protein motif:HMM:NF024770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HYD84223.1"
                     /translation="ELEIVSGWKYFGSRAGRSLERLLQGHPDRCWVLARSHRVLQRWF
                     AQKNVPALVVGTTHAGIDLPSVDIDHRALCRHAAITFLRQGHRQLALFLEKAGHGGDE
                     DSEKGFLEGMATQADALPPIIRRPSKGPGYIISELRHLEQLRVPPTGLLLSNSFSYLT
                     ALSYFAQQGRRVPRDISLISRDEEPFFSHLHPVPTRYSISPAKFAAAIRQAIKRVLEG
                     STPRRFDVRIMPDFIKGASVAAPKSSK"
     gene            840..1643
                     /locus_tag="VEA63_09225"
     CDS             840..1643
                     /locus_tag="VEA63_09225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009513742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HYD84224.1"
                     /translation="MSKLEPNRLLANIRAGRVEHCFVLGNHATPRIVDFVSGSGLFET
                     LWFDLEHFDIPTDQLAVLNLVARAHPVTTLARFHADNYQAVQRVLETGIGGIMCAMVE
                     SAAEAAQIVRWSKFNNPSPANGETIGQRGWNGGGIDARYGTVPAADYVAHQNRETVIL
                     CQIETETGLAAVDEIVAVPGVDGIFFGPGDFAHRIGKLGQIGHADVLRAMERVAASCR
                     EHAKFWGTLGIGREHYLAVKKIGAQLICPGGDVRTMNLGLRELAKTFQE"
     gene            1865..>2249
                     /locus_tag="VEA63_09230"
     CDS             1865..>2249
                     /locus_tag="VEA63_09230"
                     /inference="COORDINATES: protein motif:HMM:NF012904.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HYD84225.1"
                     /translation="MTRTESPAIRPRTQGLVAAPFTPMRADGSLNLELIPDYVAWLRG
                     NGVAGAFVGGTTGEAMSLTVEERLQLAEAWRRAAPADLKVIVHVGHTSLSDCRAMAAH
                     AKDIGADAIGCLAPFFFRPDNVSELV"
BASE COUNT          390 a          675 c          729 g          455 t
ORIGIN      
        1 gagctggaga tcgtttcggg ctggaagtat tttggttcgc gggccggtcg ctcactggaa
       61 cgtttgttgc aagggcaccc ggatcgttgc tgggtgctgg cccgctcgca tcgcgtgctt
      121 cagcgctggt ttgcccaaaa aaatgtgccg gcgctcgtgg tgggcacgac tcacgcgggg
      181 attgatttgc cgagcgtgga catcgatcac cgtgcgctct gtcgtcacgc ggcgataact
      241 ttcctgcggc agggacatcg gcagcttgcg ctgtttttgg aaaaggccgg acacggcggc
      301 gacgaggaca gcgaaaaagg atttctcgaa ggcatggcga ctcaggcaga cgcgctgccg
      361 cccatcatcc ggcggccgtc gaaaggcccc ggctatatca tttccgaatt gcgccacctc
      421 gagcagctcc gcgttccgcc gacgggactt ctgctctcca actcgttttc ttatctgacc
      481 gcgctcagtt atttcgctca gcaaggccga cgtgtgcctc gcgacatttc gttgatctcg
      541 cgcgacgagg agccgttctt ctcgcatctt catccggtgc ccacgcgcta ttcgatctcg
      601 ccggcgaagt ttgcggcggc gattcggcag gcgatcaagc gcgtgctgga gggatcgacg
      661 ccgcggcgct tcgacgttcg catcatgccg gatttcatca aaggagcctc cgtggccgca
      721 ccgaaatctt cgaagtaggg cgggcgggca acgtgcttcg cacacgtggg ctcccgagga
      781 gaccacttga cgcgagttga ggccgccgcg ctgaaatcat cgcatgtcga tctcgtccca
      841 tgtcgaaact cgaacccaat cgccttcttg cgaacatccg cgccggccgc gtcgaacact
      901 gcttcgtcct cggcaaccat gcgaccccgc ggattgttga cttcgtcagt ggcagcgggt
      961 tgttcgagac actttggttc gacctcgagc attttgacat tccgaccgat caactcgcgg
     1021 tgctgaatct cgtcgcacgc gcgcatccgg tgacgacgct cgcgcgattt cacgcggaca
     1081 attaccaggc ggtgcagcgc gtgctggaga ccggcatcgg cggcatcatg tgcgcgatgg
     1141 tggagagcgc ggccgaggcg gcgcagatcg tgcgctggtc gaagttcaac aatccgtctc
     1201 ccgccaatgg cgaaacgatt ggccagcgag gttggaacgg cggcggcatc gatgcccgtt
     1261 atgggaccgt tcctgcggcg gactacgttg cgcaccagaa ccgtgagacc gtgatcctgt
     1321 gccagatcga gacggagacc ggcttggcgg cggtggacga aatcgtcgcc gtgccgggag
     1381 tggacggcat cttctttggc cccggcgatt ttgcgcatcg catcggcaag ctcggccaga
     1441 tcggccacgc ggatgttctg cgggcaatgg agcgcgtggc ggcgtcatgc cgcgagcacg
     1501 ccaagttttg gggaacgctc ggaattggac gtgagcacta cctcgcggtg aagaagatcg
     1561 gcgcgcaact catctgtccc ggaggcgatg tgcgcacgat gaatctcggg ctgagggagc
     1621 tggcgaagac ctttcaggag taaggcgcgg tcgcacgctg cggagtttcg aaggccgagg
     1681 acccatttgc ccggctgctg gtgtctctgc tggtctgcaa tgcaggccag tattcctgcg
     1741 ttcaggtcga acgctttttc tcgttcatcg attttggcag caggcgcgcg accgccgccg
     1801 gcccagcgag gttcaaaggc ctcctgcaga tatttcctgc aaagccaaaa ttatgatctc
     1861 accgatgact cgcacggaat cgccagcgat tcggcccaga acccaaggct tggtggccgc
     1921 gcccttcacg ccgatgcgcg ccgacggctc gctcaatctg gagctcatcc cggactatgt
     1981 cgcgtggttg cgcggcaatg gtgtcgctgg cgctttcgtc ggcggcacca cgggcgaggc
     2041 catgtcgttg acggtggaag agcggctcca gcttgccgaa gcatggcgac gcgcggcgcc
     2101 agccgatctc aaagtgatcg tgcatgtcgg gcacacttca ctgtcggatt gtcgcgcgat
     2161 ggcggcgcac gcgaaggaca tcggggcgga cgccatcggg tgtcttgcgc cgttcttttt
     2221 tcggccggac aacgtgagcg aactcgtgt
//
LOCUS       DASPLT010000887         2282 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_256707, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000887 DASPLT010000000
VERSION     DASPLT010000887.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 2282)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2282)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2282
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_256707"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..925
                     /locus_tag="VEA63_09235"
     CDS             <1..925
                     /locus_tag="VEA63_09235"
                     /inference="COORDINATES: protein motif:HMM:NF017197.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF748 domain-containing protein"
                     /protein_id="HYD84226.1"
                     /translation="AIDVENFVVRSREHPEDAPLMLLRDVSLKVAPGALLTGKLGGRI
                     TVDGAEVNVVKYERVEDPTEAVEEARREMQEKKEQVARWQDTLREAFPMELTRMEVKN
                     SRLRFIDRSHQPEVDVVLERLHIVATDLQNRPREDENVLPARIQIDGAFAGGGEFEST
                     IRLNPVAEQPRFTANFALRDLQLPPLNSFLRAYANADVSRGTFEMFMEVEAQGGAYDG
                     YVKPLFKDLDFRNADDEDKNLGERVVETVVSTVASALKNDEQEQVATRAPFAGNFADN
                     DVDLWSTIATLFRNAFVQALRAGFEGQTPRK"
     gene            1063..>2282
                     /gene="guaA"
                     /locus_tag="VEA63_09240"
     CDS             1063..>2282
                     /gene="guaA"
                     /locus_tag="VEA63_09240"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HYD84227.1"
                     /translation="MPQTIAVLDFGSQLTQVIARRIRECQVYSKIYHFSTPAEKLREE
                     GVVGIILSGGPQSVYARKAPHPDAKIFELGVPILGICYGVQLMGHFLGGKVEHSQARE
                     YGHGHLTIKRPGKLFAGLPRKLRIWNSHGDKLGKLPPGFVATAVSENSPFSGIEDAKR
                     GFYGIQFHPEVFHTERGVDMIRNFLVGVCGAKQDWTTKDFIAHAVADIRTQVGKSRVI
                     LGLSGGVDSSVAAALLHKAIGKQLTCVFVDNGLIRKGEREYVEKLYAKHFKIDLRVVD
                     ASKLFLKRLKGVTEPEQKRKIIGRTFVEVFEKSLKSIGGAQFLGQGTLYPDVIESVSI
                     GNNPASLIKTHHNVGGLPERMKLKLIEPLRELFKDEVRKVGAALGLPKEVVWRQPFPG
                     PGLGVRVMGEITAA"
BASE COUNT          437 a          702 c          720 g          423 t
ORIGIN      
        1 cgcgatcgac gtggagaact tcgtcgtgcg ttcgcgcgag catcccgagg acgcgccgtt
       61 gatgctgttg cgcgatgtct cgttgaaagt tgcgcccggt gcgctgctca ccggaaagct
      121 tggcggccgc atcacggtcg acggtgcgga ggtcaacgtc gtcaaatacg aacgcgtcga
      181 ggatccgaca gaggcggtcg aagaagcgcg ccgcgagatg caggaaaaga aggagcaggt
      241 cgcgcgctgg caggacacgt tgcgcgaggc gttcccgatg gaactcacgc gaatggaagt
      301 gaagaacagc cggctgcgtt tcatcgaccg ttcacaccaa ccggaagtcg atgtcgtttt
      361 ggagcgcctt cacatcgtcg cgaccgatct gcagaatcgc ccgcgcgagg acgagaacgt
      421 gctgcccgca cgcatccaga tcgacggcgc gttcgccggc ggcggagagt tcgagtccac
      481 gattcgtttg aatccggtcg ccgagcagcc gcgcttcact gccaatttcg ctttgcgcga
      541 tctgcagttg ccgccgctca acagctttct ccgcgcctat gcgaatgccg atgtctcgcg
      601 cggcacgttc gaaatgttca tggaggtcga ggcccagggc ggggcctacg acggttacgt
      661 gaagccgctg ttcaaggacc tggatttcag aaatgcggac gacgaggata agaacctcgg
      721 cgagcgcgtc gtcgaaaccg tcgtgtcgac cgtggcctcg gccttgaaga acgacgaaca
      781 ggagcaggtc gccacgcgcg cgcccttcgc gggtaacttt gccgacaacg acgtcgatct
      841 ctggtcgacg atcgccacgc tgtttcgcaa cgcattcgtc caagcgctcc gcgccggctt
      901 tgaaggccag acgccgcgaa aataacgcag atttccgcag ctgtgggcgg ggcgtcgagg
      961 gcctggtgcg agacatgcgc tacccgccaa atgacattgc acggaatcgc gcttgcgccc
     1021 cgcgcgccgt ctgcgttgtg ctatccgttt cccacgccgc acatgcctca aaccatcgcc
     1081 gtcctagact tcggttcgca gctcacccag gtcatcgcgc gtcgcattcg cgagtgtcag
     1141 gtttactcga agatctacca tttctccacg ccggcggaga aactgcgcga ggagggcgtg
     1201 gtcggcatca tcctctccgg cggcccgcag agcgtgtatg cgcgcaaggc gccgcatccg
     1261 gacgcgaaaa ttttcgaatt gggcgttccg atcctgggga tttgctacgg ggtgcaactg
     1321 atgggtcact tcctcggcgg caaagtcgag cacagccagg ctcgcgaata cggacacggt
     1381 catctcacga tcaagcgccc cggcaagctg ttcgccggac tcccgcgcaa gcttcgcatc
     1441 tggaattccc acggcgacaa actcggcaag ctcccgccag gtttcgtcgc caccgcggtt
     1501 tccgagaact cgccgttctc cggcatcgag gatgcgaagc gcggtttcta cggcatccag
     1561 tttcacccgg aggttttcca caccgagcgc ggcgtggaca tgattcgcaa tttcctcgtc
     1621 ggcgtttgcg gcgcgaaaca ggactggacg acgaaggatt tcatcgcgca cgccgtcgcc
     1681 gacatccgca cccaagtcgg caagtcgcgc gtgatcctcg ggctttccgg cggcgtcgat
     1741 tcgtctgtgg cggcggcgct gttgcacaag gcgatcggca agcagctcac gtgcgtcttc
     1801 gtggacaacg gcctcatccg caaaggcgag cgcgaatatg tcgagaagct ctacgcgaag
     1861 catttcaaaa tcgacctgcg tgtcgtcgac gcctcgaagc ttttcctgaa gcgtttgaag
     1921 ggcgtcaccg aacccgagca aaagcggaag atcatcgggc gcacgtttgt cgaagtgttc
     1981 gagaagtccc tgaagtcgat cggcggcgcg cagtttcttg ggcagggcac gctttatccg
     2041 gacgtgatcg agagcgtgtc gatcggtaac aatcccgcct cgctcatcaa gacgcatcac
     2101 aacgtcggcg gtctgccgga gcggatgaag ctcaagctca tcgagccgct gcgcgagttg
     2161 ttcaaggacg aggtccgcaa agtgggcgcg gcgctcggtc tgcccaagga agttgtctgg
     2221 cggcagccgt tccccggccc gggtctcggc gtgcgcgtga tgggcgaaat cacggccgcg
     2281 aa
//
LOCUS       DASPLT010000888         1984 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_50459, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000888 DASPLT010000000
VERSION     DASPLT010000888.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1984)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1984)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1984
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_50459"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..522
                     /locus_tag="VEA63_09245"
     CDS             <1..522
                     /locus_tag="VEA63_09245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoF synthetase"
                     /protein_id="HYD84228.1"
                     /translation="VDLYGSTEAGYLFVGEAFKDNSQVIEANAFIELAPWRTNLPDVF
                     QIYVTTRDREAMPLLRYHTGDIVQKFPTGFRLLGREGSLYSRADGSLVSTADIDAALP
                     KEFACWHYSLIQTSEARWDFHYVADEVAPAGIEQAIARTLGDGVRVSAFRRRVLAPAA
                     SGKFALLKPLAAK"
     gene            698..1459
                     /locus_tag="VEA63_09250"
     CDS             698..1459
                     /locus_tag="VEA63_09250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b subunit"
                     /protein_id="HYD84229.1"
                     /translation="MNFFRSTIGRKILMAVTGLILIGFVVGHLVGNLQVFSGPDKLNG
                     YAHFLQSLGPLLWAARIGLLVAVGIHVWAATVLTLENHAARNVGYSVKHTIRATLASR
                     MMRWSGYVVLAFIVYHVAHFTLGVAQADTFKTALPEYTMQSEYHVAGFPVVDAGAVVH
                     DVHSMVILGFQNVIVSIFYIIAVGLLSIHLLHGTDSMFQTLGLRSRRWSGALRTICIV
                     FAAAYFVGNLAIPGAVLLGKLQPHGESAVVAAAQR"
     gene            1475..>1984
                     /locus_tag="VEA63_09255"
     CDS             1475..>1984
                     /locus_tag="VEA63_09255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007359765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HYD84230.1"
                     /translation="MAQLDSRIPPGPLADKWRKHKADIKLVNPANKRKYEVIVVGAGL
                     AGGSAAATLAELGYKVKSFVYHDSPRRAHSIAAQGGINAAKNYQNDGDSVYRLFYDTI
                     KGGDYRSREANVHRLAEVSVNIIDQCVAQGVPFAREYGGLLANRSFGGAQVSRTFYAR
                     GQTGQQLLLG"
BASE COUNT          341 a          572 c          667 g          404 t
ORIGIN      
        1 gtcgatctct acggctcgac cgaggcgggg tatttgtttg tcggcgaagc gttcaaagac
       61 aactcgcagg tgatcgaggc caacgcgttt atcgaactgg cgccgtggcg gacgaatctg
      121 ccggatgtgt ttcaaatcta cgtgacgacg cgcgaccgcg aggcgatgcc gctgctgcgt
      181 tatcacaccg gcgacatcgt gcagaagttt ccgacgggct ttcggttgct cgggcgcgag
      241 ggtagtttgt attcgcgcgc ggatggatcg ctggtgtcga ccgctgacat cgatgcggcg
      301 ttgccgaagg agttcgcgtg ctggcactac tcgctgatcc agacgagcga ggcgcgctgg
      361 gattttcatt acgtggcgga cgaagtcgcg ccggcgggga tcgagcaggc gatcgcgcgg
      421 acgctgggcg acggcgtgcg ggtgagcgcg tttcgccggc gggtgctggc gccggcggcg
      481 agtggaaagt tcgcgttgtt gaagccgctg gcggcgaagt aaacgcacgg acgtgggagc
      541 gatggagacg cggacaacga gatttccgtt atcttctcca aaaagttagg gagcctaata
      601 tgcgccccgc cgcagggccg ctcctttaca acgcatcggg ggcagccgaa gttagcctcg
      661 tttagcgact gcgttcccct aatccctgat tttcgcgatg aatttttttc gctccaccat
      721 cggccgcaag atcctcatgg ccgtgacggg cctgatcctg atcggcttcg tggtcggcca
      781 tctcgtcggc aacctccagg tgttttccgg gccggacaag ctgaacggtt acgcgcattt
      841 tctccaatcg ctcggcccgt tgctgtgggc cgcgcggatc ggcttgctgg tggcggtggg
      901 aattcacgtg tgggcagcca cggtgttgac gttggaaaat catgcggcgc ggaatgtcgg
      961 ttacagcgtg aagcacacga ttcgcgcgac gctcgcgtca cgaatgatgc gctggagcgg
     1021 ttacgtggtg ctcgcgttta tcgtgtatca cgtggcgcat ttcacgctcg gcgtggcgca
     1081 ggcggatacg ttcaagacgg cgctgccgga gtatacgatg cagagcgagt atcacgtcgc
     1141 gggatttccg gtggtggacg cgggggcggt cgtgcacgac gtgcactcga tggtgatcct
     1201 cggtttccag aacgtgatcg tgtcgatttt ctatatcatc gccgtgggat tgctgagcat
     1261 ccatctgctg cacggcaccg acagcatgtt tcagacgctc gggctgcgaa gccggcggtg
     1321 gtcgggtgcg ctgcgcacga tctgcatcgt tttcgccgcg gcgtattttg tcggcaacct
     1381 cgcgatcccc ggcgccgtgt tgctcgggaa gcttcaaccg cacggcgagt ccgccgtggt
     1441 cgccgctgct caacgctaaa atcttttccg cgccatggcc caactcgact ctcgcatccc
     1501 gcccggcccg ctcgctgaca agtggcgcaa gcacaaggcg gacatcaagc tcgtcaatcc
     1561 tgccaacaag cggaaatacg aggtgatcgt tgtcggcgcc ggtctcgccg gcggctccgc
     1621 ggccgcgacg ctcgccgagc tcggctacaa ggtgaagagc ttcgtgtatc acgacagccc
     1681 gcggcgggcg cactcgatcg cggcgcaggg cggcatcaac gccgcgaaga actaccagaa
     1741 cgacggcgac agcgtgtatc gacttttcta cgacacgatc aagggcgggg actaccgttc
     1801 gcgcgaagcg aacgtgcacc ggctggctga agtgtcggtg aacatcatcg atcagtgcgt
     1861 ggcgcaaggc gtgcccttcg cgcgcgaata cggcggcttg ctggcgaacc ggtcgtttgg
     1921 tggggcgcag gtttcgcgga cattttatgc gcgcggccaa actggccaac aattgctgct
     1981 cggc
//
LOCUS       DASPLT010000889         1339 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_134253, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000889 DASPLT010000000
VERSION     DASPLT010000889.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1339)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1339)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1339
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_134253"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1339)
                     /locus_tag="VEA63_09260"
     CDS             complement(<1..>1339)
                     /locus_tag="VEA63_09260"
                     /inference="COORDINATES: protein motif:HMM:NF013377.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004555 - alpha,alpha-trehalase activity
                     [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="trehalase family glycosidase"
                     /protein_id="HYD84231.1"
                     /translation="KRALPNGGFYYLDQVEDTWESGMDEGVRYHPRPAKLSAAVDACA
                     HVYMLYDHAERWSRRLGLPAEAWKRKADALRDFIRTELWDEQTSFFYDHWTVREPGQR
                     SLAFEGMWPVVVGAATAAQARRVIDEHLLNPKEFFTPHPLATVAASDPRFELRMWRGP
                     AWNCMTYWAARGCLRYGNAEGARRLLEAALDATAVQFERTGAIWEFYHPLLGDQRELK
                     RKPTGRNEPCRDYLGHNPLFAMVDLWRSAGGTAAAIAPAETEVQQRGVVDRLPVFIDR
                     LKERTEHSWSWTSGTFSDFAEWRRLAREKVIASFQTPPPSASWRASMVEEEDRGSYVV
                     RRIAFNVTADSRVFALMTVPKGSGPFPAVLLLHDHGGRFDIGKEKVIRPLATDVAKLA
                     SAEEWVAKYYGGRFLGDELAQRGYVCLSVDALNWSDRGGGGHDGQQSLASNLM"
BASE COUNT          252 a          481 c          370 g          236 t
ORIGIN      
        1 tgcatcaggt tcgacgcgag cgactgctgg ccgtcatgcc cgccgccgcc gcggtccgac
       61 cagttgagcg catcaacgga taaacacacg taaccgcgct gcgcgagttc gtcgcccaga
      121 aagcggccgc cataatattt cgcgacccac tcttccgccg aggcgagttt ggcgacatcc
      181 gtcgcgagcg gccgaatcac cttttccttg ccgatatcga agcgcccacc gtgatcatgc
      241 agcagcaaca cggcgggaaa cggcccgctt cctttcggca ccgtcatgag cgcgaagacc
      301 cggctgtccg ccgtcacgtt gaacgcgatc cttcgcacaa catagctgcc gcgatcttcc
      361 tcctcgacca tgctcgcccg ccatgacgcc gacggcggcg gagtttgaaa gctcgcgatg
      421 actttctcgc gcgcgagacg ccgccactcc gcgaaatcgc tgaacgttcc cgaggtccac
      481 gaccacgaat gctccgtgcg ctccttcaac cgatcgatga aaacgggcag tcgatcgacg
      541 accccacgtt gctgaacctc cgtttccgcc ggcgcaatcg cagccgccgt cccgcccgcg
      601 ctccgccaca aatccaccat cgcgaaaagc ggattgtgcc cgaggtaatc gcgacacggt
      661 tcgttgcggc cggtcggttt ccgcttcaac tcgcgctgat cgccgaggag cggatgataa
      721 aactcccaga tcgcgcccgt ccgttcgaac tgcaccgccg tggcgtcgag cgcggcctcg
      781 agcagtcgtc gcgctccctc cgcgttaccg tagcgcaggc agccgcgcgc cgcccagtaa
      841 gtcatgcaat tccaagccgg cccgcgccac atccgcaact cgaagcgcgg atcggatgcg
      901 gccaccgtgg cgagtggatg cggcgtgaaa aattccttcg ggttgagcaa atgttcatcg
      961 ataacgcgtc gtgcctgcgc ggctgtcgcg gcgccgacta caaccggcca catgccttca
     1021 aacgcgagcg atcgttggcc cggctcgcgc acggtccagt gatcgtagaa gaaactcgtt
     1081 tgttcatccc acaattccgt gcgaatgaag tcccgcagcg cgtcggcctt tcgcttccat
     1141 gcctccgccg gcagacccaa ccgccggctc cagcgctccg catgatcata gagcatgtag
     1201 acatgcgcgc acgcatccac cgcagccgac aacttcgccg gccgcggatg atagcggaca
     1261 ccttcgtcca tcccgctttc ccatgtgtcc tccacttgat cgaggtaata aaagccgccg
     1321 ttcggcagcg ctcgcttcg
//
LOCUS       DASPLT010000890         2272 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_50469, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000890 DASPLT010000000
VERSION     DASPLT010000890.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 2272)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2272)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2272
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_50469"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(513..968)
                     /locus_tag="VEA63_09265"
     CDS             complement(513..968)
                     /locus_tag="VEA63_09265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD84232.1"
                     /translation="MKVSFTTKEYTRLLELVHLGLSVAGARADDPATMPERYADLAQK
                     VFGLAEVFGCADLVEADVNGELFPNERLTEGVAREKMEAFAEDVFWGELATRLALRDL
                     RTESGSAALEEEELSPENEEKLAEREDAYWREFETHGVDHLVLLRGGRG"
     gene            1093..>2272
                     /locus_tag="VEA63_09270"
     CDS             1093..>2272
                     /locus_tag="VEA63_09270"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HYD84233.1"
                     /translation="MRQPSKHPSTSFDPASTTARNQNLDTSHRRRTHRISREYYSWMK
                     AGLHATQALRVLRRRRQRPTTTHTEHLLSSTMRNLARLVAAAAFVCPGLLVAQTSTAP
                     AGRAASPDEPIVVLDKFVVEEQGEDPNYVLPDGPSASSFGFNKSLLDTPRTVSMISSE
                     IIDKMGLSAVEDLVRVVPGVYTLTRYGIQGNVDVRDVPADLFFRGMKRVNMEGYARTV
                     LAAMDTIEVVKGPPSPIYGMGKIGGYTNMVPKSGRAKTGGYLPKAQGFAQGITGAYNR
                     NELSAGGGGPFTVGGKTGGYYVYGLMEDSDTFTDGVPVKTKMVQVAASLDRFIGPFRL
                     DTGINLQSTRTAGSVLGRVTQDVIDDGTYIRGTPLINLDLNGNGSIGYLEYQQASPAQ
                     G"
BASE COUNT          442 a          851 c          593 g          386 t
ORIGIN      
        1 ccctcgggat tcatccacgc caccggcgca tgcgtcacat cctgcgctga caacgaaatg
       61 acaaaaatcg ccgccaccca ccacgcgccg cgacgaacaa acgaactgat tcgcttcatt
      121 gcgacggagc ctgccgccac cgcaaaaccc ggtcaatgcg cgacgccaat tccctgcgtc
      181 ttacccgctc aaccgcgtga gcgtgtcttt tctacaggag aaagcagagg tccgcagaga
      241 ccagccgcga aactgacctg acgcgaaaga atcgcgcaga aacaaagagg attgttcaga
      301 cctccgggtc ttttgactct gtcgcgaagg accatggctg cggtgcctcc tcgatcatca
      361 cctctgcact ctccgctgcc tcctgtaaat ggatcggtcc tcaggatcga ccgcgaagat
      421 cagcttcctt ccgcagctca tccattcagc ggcgctcgtt tcgctgatcg gtctttccac
      481 caacgaagcg agcatggctg agctgatccc gctcaccccc gtccgccgcg caacagcacc
      541 agatggtcga cgccgtgcgt ctcgaactcc cgccaatacg cgtcctcgcg ctccgccagt
      601 ttctcctcgt tctccggcga cagctcctcc tcctccaacg ccgcgcttcc cgactccgtc
      661 cgcagatcgc gcaacgccag ccgcgtcgcc agctcgcccc agaaaacatc ctccgcgaac
      721 gcctccatct tttcacgcgc gacgccctcg gtgagccgct cgttgggaaa aagttcgccg
      781 ttcacatccg cctcgacgag gtccgcacag ccaaacacct ccgcgagccc aaacaccttc
      841 tgcgcgagat cggcgtaacg ctccggcatc gtcgccggat cgtcggcccg cgcgcccgcc
      901 acggacagcc cgaggtgcac cagctcgagc aaccgcgtgt attccttcgt cgtaaacgag
      961 actttcatcc tcgcactgca cgcggcccgt gacaccgccg caaggccgga gtgaaccgtc
     1021 ccgtcaacga cgctcagcca ccgcgaacgg cctccaggta gggcgcggtc tcccacccgc
     1081 cctcgctgcg ccatgcgcca gccatcgaag cacccgtcca catccttcga tcccgccagc
     1141 acgaccgcgc gcaaccaaaa tctggatacg tcgcaccgcc gacgcaccca tcggatttcg
     1201 cgcgaatact attcatggat gaaagccggt ctgcatgcca cgcaggcact tcgcgtgctc
     1261 cgtcgccggc gtcagcgacc caccaccacg cacacggaac acctcctttc ttcgaccatg
     1321 agaaacctcg cgcgtctcgt ggccgcggca gccttcgtct gccccggcct cctcgttgcc
     1381 caaacctcga ccgctcccgc cggcagggcg gcgtcacccg acgagccgat cgttgtgctc
     1441 gataaattcg tcgtcgagga acagggcgaa gatcccaact acgtgctgcc cgacggccca
     1501 tcggcctcat cgttcggctt caacaagagc ctgctcgata cgccgcgcac cgtctcgatg
     1561 atcagctcgg agatcatcga caagatgggc ctctccgccg ttgaggatct cgtgcgtgtc
     1621 gtccccggcg tttacacgct cacccgctac ggcatccagg gcaacgtcga cgtccgcgac
     1681 gtccccgcgg atcttttttt ccgcggcatg aagcgcgtca acatggaggg ctacgcgcgc
     1741 accgtgctcg ccgccatgga tacgatcgag gtcgtgaaag gcccgccgtc gccgatctac
     1801 ggcatgggaa aaatcggcgg ctataccaac atggtgccga agtccggccg cgcaaaaacc
     1861 ggcggctacc ttccgaaagc gcagggcttc gcccaaggca tcaccggcgc ctataatcgc
     1921 aacgaactct ccgccggcgg cggcggcccg ttcaccgtcg gcggcaaaac cggtggctat
     1981 tatgtctacg gtttgatgga ggactccgac acgttcaccg acggcgtgcc ggtgaaaacg
     2041 aagatggtgc aggtcgccgc cagcctcgac cgcttcatcg gtcctttccg tttggataca
     2101 ggcatcaatc tccagagcac ccgcaccgcc ggctccgtgc tcggccgcgt cacccaggac
     2161 gtgatcgacg acggcaccta catccgcggc accccgttga tcaatctcga cctcaacggc
     2221 aacgggtcca tcggctatct cgaataccag caagcctcgc ccgcgcaagg cc
//
LOCUS       DASPLT010000891         1014 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_175179, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000891 DASPLT010000000
VERSION     DASPLT010000891.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1014)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1014)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1014
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_175179"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..448
                     /locus_tag="VEA63_09275"
     CDS             <1..448
                     /locus_tag="VEA63_09275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD84234.1"
                     /translation="KTSMVKSSATKKIAKTQKQNNPSRSFSRLLRFLRLRRDRSDRLN
                     LRKPNKRSARRRSGGARTHSLTSPPPKNQRKLAVKNSAGPRRYHRPTTATTLAIPLRR
                     FKTQNFYGQILSHQKERKGTKNRTIPPDHFRAFCAFLRLHSIDLLE"
     gene            complement(558..>1014)
                     /locus_tag="VEA63_09280"
     CDS             complement(558..>1014)
                     /locus_tag="VEA63_09280"
                     /inference="COORDINATES: protein motif:HMM:NF015506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HYD84235.1"
                     /translation="AALEASVFERPMASGRTTSVTLAKRAEFKAVPRDLAADADALAR
                     VVTALRAGSVQSAKGLDGKLVPLYRVAPTYPAALKEGGSPAGRAEIDFVIDREGRARL
                     PRIVSATHEEFGWAAATAVAQWVFQAPTRGGEPTEVKVRIPFDFAPPAG"
BASE COUNT          228 a          388 c          223 g          175 t
ORIGIN      
        1 caaaacttct atggtcaaat cctcagccac caaaaagatc gcaaagacac aaaaacagaa
       61 caatccctcc cgatcatttt cgcgcctcct gcgctttttg cggctacgtc gcgatcgctc
      121 tgaccgactg aatctccgca aacccaacaa gcgttccgcg cgtcgccgat caggcggagc
      181 acgcactcat tcgctcacct caccgcctcc gaaaaatcag cgcaaattag cggttaaaaa
      241 ttctgccggt ccaaggcgat accaccgtcc gacgaccgcc acgacactcg ctatccctct
      301 tcgtcgtttc aaaactcaaa acttctatgg tcaaatcctc agccaccaaa aagagcgcaa
      361 aggcacaaaa aacagaacaa tccctcccga tcattttcgc gccttctgcg cttttttgcg
      421 gctacactcg atcgatctgc tcgagtgaat ctccgcaaac caacaagcgt tccgcgcgtc
      481 gccgatcagg cggagcacgc actccttcgc tcacctcacc gtctccgaaa aatcagcgca
      541 aattagcggt taaaaactca gcccgccggc ggcgcaaaat cgaacgggat gcgcaccttt
      601 acctccgtcg gctctccgcc gcgcgtcggc gcctgaaaca cccattgcgc gaccgccgtc
      661 gccgccgccc aaccgaattc ttcgtgggtc gccgaaacga tccgcggcaa acgcgcgcgc
      721 ccctcgcgat cgatcacgaa atcgatttcc gcgcgccccg ccggacttcc gccttccttc
      781 aacgccgccg gatacgtcgg cgccacgcga tacaacggca ccagctttcc atccaagcct
      841 ttcgccgact gcacgctgcc cgcacgcaac gccgtcacca cccgcgccag cgcatccgcg
      901 tccgccgcca gatctcgcgg caccgccttg aactccgcac gcttcgccag cgtcaccgag
      961 gtggtgcgac cgctcgccat cggccgctcg aaaacactcg cctcgagcgc cgcg
//
LOCUS       DASPLT010000892         1255 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_175182, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000892 DASPLT010000000
VERSION     DASPLT010000892.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1255)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1255)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1255
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_175182"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..721)
                     /locus_tag="VEA63_09285"
     CDS             complement(<1..721)
                     /locus_tag="VEA63_09285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HYD84236.1"
                     /translation="MTRLASDRRLYPFFQFACGLLLLPLSLRALDPAKSVFQFNCQNW
                     SRHNGLPADKIAAVAQTADGFIWLGTQHGLTRFDGLDFSVVPIALPEAKGHDVRALYA
                     AHRGGLWFAIDQGGFGYFDGRVFTPVGDARWSQPGMSATAILERRNGAIWTGSIFGAA
                     RWVKDDPQRTFFEETFENALLLAEDPQGRVWCGAAERGLRYFEKGKWTAIADEELSRR
                     NINALAFTPDGDIWVGTNQGLR"
     gene            complement(718..>1255)
                     /locus_tag="VEA63_09290"
     CDS             complement(718..>1255)
                     /locus_tag="VEA63_09290"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HYD84237.1"
                     /translation="GGWLELTSEVGVGTTFTVFLPEADARTGSAVKTAAPETAVSGGR
                     ETLLIVEDEPSVREFTAAVLRPLGYRLLQARSGVDALELWKRHSAQIDLLLTDMVMPD
                     QLTGPQLATALVAQKPSLAVVFTSGYSQESIGRVFPPEKGARFIHKPYSPRDLAKIVR
                     ETLDRRKRVSAAASASMR"
BASE COUNT          222 a          366 c          464 g          203 t
ORIGIN      
        1 ggcgcaagcc ttggttggtg ccgacccaga tgtcgccgtc cggcgtgaag gcgagcgcgt
       61 tgatgttgcg ccgcgacagc tcttcgtcgg cgatagcggt ccattttcct ttctcgaaat
      121 aacgcaggcc gcgttcggcc gcgccgcacc agacgcggcc ctgcgggtct tcggcgagca
      181 gaagggcgtt ctcgaaggtc tcctcgaaga aggttcgttg cggatcgtcc ttcacccagc
      241 gagctgcgcc gaagatggag ccggtccaaa tggcaccgtt gcgcctttcg agaatggcgg
      301 tggcagacat gccgggctgc gaccagcggg cgtcaccgac cggggtgaac acacgaccgt
      361 cgaaataccc gaagccgcct tggtcgattg cgaaccaaag gccgccgcgg tgcgcggcgt
      421 aaagcgcgcg cacgtcgtgc cctttagcct cgggaagcgc gatgggcacg acggagaaat
      481 cgagcccgtc gaaacgggtg agtccgtgtt gcgtgccgag ccagatgaag ccgtcggcgg
      541 tctgcgcgac ggcggcgatc ttgtcggcgg gcaggccgtt gtggcgcgac cagttttggc
      601 agttgaactg aaaaacggat tttgcgggat cgagcgcgcg caacgagagc ggcagcagca
      661 acaggccgca ggcgaactga aaaaacggat acagccgacg atcggacgca aggcgagtca
      721 tcgcatggaa gcggaggcgg cggcgctcac gcgtttgcgg cggtcgagcg tttcgcgaac
      781 gatcttcgcg agatcgcggg gcgagtaggg cttgtggatg aagcgcgcgc ctttttcggg
      841 agggaacacg cgaccgatgg actcctggct gtagccgctg gtgaacacga ccgcgaggga
      901 gggtttttgc gcgacgagcg cggtggcgag ttgcgggccg gtgagctggt cgggcatcac
      961 catgtcggtg agcaggagat cgatctgcgc ggagtggcgt ttccagagtt cgagcgcgtc
     1021 gacgccggag cgcgcctgga ggaggcgata gccgagcgga cgcaggacgg cggcggtgaa
     1081 ctcgcggacg ctcggctcgt cttcgacgat caacagcgtc tcgcgtccgc cgctgacggc
     1141 ggtttccgga gcggcggttt tgacggccga accggtgcgc gcgtcggcct ccggcagaaa
     1201 aaccgtgaac gtggtgccga cgccgacttc gctggtcagc tcgagccacc cgccg
//
LOCUS       DASPLT010000893         1161 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_134302, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000893 DASPLT010000000
VERSION     DASPLT010000893.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1161)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1161)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1161
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_134302"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            293..>1161
                     /locus_tag="VEA63_09295"
     CDS             293..>1161
                     /locus_tag="VEA63_09295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD84238.1"
                     /translation="MSKEARRRNLWIVAVLLIVAALLFLVRCSRKNDVPAATEQAAPA
                     PAVAPAQASAPVEGTTPAAEVLTPATLEAPQQVGAGAAFSVVWTGPDNVQDFITIVKR
                     DAADSAYANYGDTKGGSPLALTAPVEAGEWELRYVTARSRTVLARRPLTVVAAEATLE
                     AAEEVVLGATLSVAWTGPNNVQDYVTIVAKGTPDGRYGNHAETKQGSPLTLTVPPEAG
                     EAELRYVTGQGGKVLARRALRIVAPEIRMSAPDEAVAGAVVTVEWAGPNNAGDYITLV
                     LKETKDGQYGNYT"
BASE COUNT          229 a          275 c          472 g          185 t
ORIGIN      
        1 aggcctcaca cacggcggcg aaaaattctc cgccgaaacc gagcaaggcg tggatcacag
       61 agtgcaccga gcgctgacac agaggaagcg ggggaggcga caaggtgttg gctgcgttgt
      121 gtgcggatgg atggatgcaa ataaagaccg aaaatcctgg ttcggattgg gcagatagct
      181 ctgcgaattc ggtgtcggag ccaggttttt gcggggtttt gcggacagag cattcagggg
      241 ttgccgatgg gtctgggatt gaggcgaatc gaggcggcgc tagatcgaga ggatgagcaa
      301 agaagcgcgt cggcggaatc tgtggatcgt ggcggtgctg ctgatcgtgg cggccctgct
      361 tttcttggtg cggtgttcga gaaaaaacga cgtgcccgcc gcgaccgagc aggccgcgcc
      421 ggcgcctgcc gttgcgccag cgcaggcgag tgcgccagtg gagggaacaa cgccggcggc
      481 ggaagtgctg acgcctgcga cgctggaggc gccgcaacag gtcggggcgg gggcggcgtt
      541 ttccgtggtt tggacgggac cggacaacgt gcaggatttc atcacgatcg tgaaacgcga
      601 tgcggcggac agcgcgtatg cgaattatgg agacacgaag ggcggaagtc cgttggcgct
      661 cacggcgccg gtcgaagcgg gcgaatggga actgcgttat gtgacggcgc ggtcgcggac
      721 ggtgctggcg cgacggccgc tgacggtggt ggccgcggag gccacgctcg aggcagcgga
      781 ggaagtggtg ttgggagcga cgttgtcggt ggcgtggacg ggaccgaaca atgtgcagga
      841 ttacgtgacg atcgtggcga agggcacgcc cgacgggcgg tatggaaacc atgcggagac
      901 gaagcaagga tcgcccttga cgctgacagt gccgccggaa gcgggagaag cggagctgcg
      961 ctacgtgacg gggcagggag ggaaggtgct cgcgcggcgg gcgttgagga ttgttgcgcc
     1021 ggagattcgg atgtcggcgc cggacgaggc ggtcgcgggc gcggtcgtga cggtggagtg
     1081 ggcgggtccg aacaatgctg gcgactacat cacgctcgtg ttgaaggaga cgaaagacgg
     1141 tcagtatggg aattacacgg a
//
LOCUS       DASPLT010000894         1181 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_154164, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000894 DASPLT010000000
VERSION     DASPLT010000894.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1181)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1181)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1181
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_154164"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..872)
                     /gene="bshA"
                     /locus_tag="VEA63_09300"
     CDS             complement(<1..872)
                     /gene="bshA"
                     /locus_tag="VEA63_09300"
                     /inference="COORDINATES: protein motif:HMM:TIGR03999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071793 - bacillithiol biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-alpha-D-glucosaminyl L-malate synthase
                     BshA"
                     /protein_id="HYD84239.1"
                     /translation="MTRPLKIGITCYPSVGGSGILASALGEELARRGHEIHFISYEKP
                     FRISIDASHLFFHPVVVNGYDLFKYPDYTLPLSVKMAEVSRDHQLDILHVHYAVPHAT
                     AAILALEMLPTDRRPKLITTLHGTDTTLLGNDPGYGPAVRHALQRSDAVTAVSRFLER
                     ETHRVLGPDCPIEVIHNFFSARPTARPRDVVRRELGVATRETLLLHLSNLRPVKRIDR
                     LLETIARIRRSDAFKLLILAGGDFAQFGDEVRRLGIEDRIVVRQNVFDIEDYLNAADI
                     GLFTSDSESFCLSIL"
     gene            complement(950..>1181)
                     /locus_tag="VEA63_09305"
     CDS             complement(950..>1181)
                     /locus_tag="VEA63_09305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD84240.1"
                     /translation="LPILVDVSPPDVLAAWTAAMEAHASQQQTRNYAELQLTRARLQG
                     LRAGVSHAIPLWPNDPLVVDSLGRFTKSARRF"
BASE COUNT          234 a          282 c          460 g          205 t
ORIGIN      
        1 aggatactga gacagaaact ttcggaatcg gaggtgaaca ggccgatgtc ggcggcgttc
       61 aggtagtctt cgatgtcgaa gacattttgg cggacgacga ttcgatcctc gatgccgagc
      121 cggcgcacct cgtcgccgaa ctgtgcgaaa tcgccgcccg caaggatgag caacttgaat
      181 gcatccgaac ggcggatacg cgcgatggtt tcgagcaggc ggtcgatgcg tttgaccggg
      241 cgcaaattcg aaaggtggag gagcaacgtt tcgcgggtgg cgacgccgag ttcgcggcgg
      301 accacgtcgc gcggacgcgc agtggggcgg gcggagaaga aattatggat gacctcgatc
      361 gggcaatcgg gaccaaggac gcggtgggtt tcgcgttcga ggaagcgcga gacggcggtg
      421 acggcatcgg agcgttggag cgcgtggcgc acggcggggc catagccggg atcgttgccg
      481 agcagcgtgg tgtcggtgcc gtggagcgtc gtgatgagct tggggcggcg atcggtcggc
      541 agcatctcga gcgcgaggat ggcggcggtc gcgtgaggca cggcgtaatg gacgtgcagg
      601 atgtcgagtt ggtggtcgcg gctgacctcg gccattttga ccgagagcgg aagcgtgtag
      661 tcgggatatt tgaaaaggtc gtagccgttg acgacgacgg gatggaaaaa caggtgcgag
      721 gcgtcgatcg agatgcggaa gggcttttcg tagctgatga aatggatctc gtgtccgcgg
      781 cgcgcgagtt cctcgccgag cgcggaggcg aggatgccgc tgccgccgac ggaaggataa
      841 caggtgatgc cgattttgag aggacgcgtc atgaaggttg cccgttaatc acgcgaatag
      901 acgcgatcac gcgggaatcg atgcacgtga aacgatgtcg ccggcgactt caaaagcgcc
      961 gcgctgattt ggtgaaacgg ccgagcgaat cgacgacgag cggatcgttt ggccagagag
     1021 gaatggcgtg gctcacgccg gcgcggaggc cttggagacg agcgcgcgtg agctggagct
     1081 cggcgtagtt gcgcgtttgt tgttgggaag cgtgcgcttc catcgcggcg gtccaggcgg
     1141 cgaggacgtc gggcggtgaa acgtcgacga gaatcgggag t
//
LOCUS       DASPLT010000895         1558 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_50539, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000895 DASPLT010000000
VERSION     DASPLT010000895.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1558)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1558)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1558
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_50539"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..722
                     /locus_tag="VEA63_09310"
     CDS             <1..722
                     /locus_tag="VEA63_09310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sulfite reductase subunit alpha"
                     /protein_id="HYD84241.1"
                     /translation="DLLAEFPSAKLTPQELVDHQRKLMPRLYSIASSPKVYPTEIHLT
                     VAVVRYETNHRERVGVCSSFLADRVQVGTTPTPVFVSHSHFGPPEDGAKDVIMVGPGT
                     GIAPFRAFVQDRIATGAAGRNWVFFGDQHRATDFLYEEEWLEWLKEGKIARLDTAFSR
                     DQVHKIYVQDRMRENAAELWAWIAGGGYFYVCGDAKRMAKDVDVALHDVVAQQGGMDA
                     AAAADYVKQMKKDKRYQRDVY"
     gene            855..>1558
                     /gene="fabG"
                     /locus_tag="VEA63_09315"
     CDS             855..>1558
                     /gene="fabG"
                     /locus_tag="VEA63_09315"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HYD84242.1"
                     /translation="MLTFNNRTALVTGAGRGIGKAIAETLARHGVTVICVSKSAESCG
                     AVATAIAAAGGKAKALAVDVADGAAVAQAAEALLKEFPTIDILVNNAGITRDGLLFRM
                     SEADWNAVITTNLSSCFHWTKALARPMTRARWGRIVNITSVSGIMGNAGQANYAAAKA
                     GMIGLTKTLAREFASRSVTVNAVAPGFIKTDMTTEFVNNPEVSAKILEAVPLKRFGEA
                     ADIANMTAYLCSEESS"
BASE COUNT          296 a          397 c          569 g          296 t
ORIGIN      
        1 tcgatctgct ggcggagttt cccagcgcga aactgacgcc gcaggagttg gtggatcacc
       61 agcgcaagct gatgccgcgg ctgtattcga tcgcgtcgtc gccgaaggtt tatccgacgg
      121 agattcatct cacggtcgcg gtcgtgcgct acgaaacgaa ccatcgcgag cgcgtgggcg
      181 tgtgctcgag tttcctcgcg gatcgcgtgc aagtgggaac gacgccgacg ccggtgtttg
      241 tgtcacattc gcatttcggc ccgccggagg acggcgcgaa agacgtgatc atggtaggtc
      301 cgggcacggg catcgcgccg ttccgcgcgt ttgtgcagga tcggatcgcg actggcgcgg
      361 cggggcgtaa ctgggtgttc ttcggcgatc agcatcgggc gaccgatttt ctctacgaag
      421 aggagtggct cgagtggttg aaggaaggaa agatcgcgcg actcgacacg gcgttctcgc
      481 gcgaccaagt gcacaagatc tacgtgcaag atcgcatgcg ggaaaacgcg gcggaactct
      541 gggcgtggat cgcgggcggg ggctatttct atgtctgcgg cgacgcgaag cggatggcga
      601 aggacgtgga tgtggcgctg cacgacgtcg tggcgcagca gggcggcatg gacgcggccg
      661 cggcggcgga ctacgtgaag cagatgaaga aggacaagcg ttaccagcgc gacgtgtatt
      721 gagggggaag tcgcgggtag tgagtagtga gtagcgagta gggcggaagc gcggtggatg
      781 tgtggcgagc gcgggagtgt tctgaaattt tctactcgct acgcgctact cactacccgc
      841 tacttttcgc tctcatgctt acgttcaaca atcgcactgc actcgtgacc ggagccggtc
      901 gcggtatcgg caaagccatc gctgaaacgc tcgcgcgtca tggcgtgacg gtgatctgtg
      961 tttcgaagtc ggcggagtcc tgcggcgcgg ttgcgaccgc aatcgcggcg gccggtggaa
     1021 aggcgaaggc gctggcggtc gacgtcgcgg atggagcggc ggttgcgcag gcggcggagg
     1081 ctttgctgaa ggagtttccg acgatcgata ttttggtgaa caacgcgggg atcacgcgcg
     1141 acggtttgct ctttcggatg agcgaggctg actggaacgc ggtgatcacg acgaatcttt
     1201 cgagttgctt tcactggacg aaggcgctgg cccggccgat gacgcgggcg cgttggggcc
     1261 gcatcgtaaa catcacctcg gtgagcggta ttatgggaaa tgcgggacag gcgaactacg
     1321 cggcggcgaa ggcgggaatg attgggttga cgaagacgct cgcgcgcgag ttcgcgagcc
     1381 ggagcgtgac ggtcaatgcc gttgcgccgg gcttcatcaa gacggacatg acgacggagt
     1441 tcgtgaacaa tccggaggtt tcagcgaaga ttttggaggc cgtgccgctc aaacgtttcg
     1501 gcgaagcggc ggacatcgcg aacatgacgg cttacctctg ttcagaggag tccagcta
//
LOCUS       DASPLT010000896         1003 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_195898, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000896 DASPLT010000000
VERSION     DASPLT010000896.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1003)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1003)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1003
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_195898"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..200)
                     /locus_tag="VEA63_09320"
     CDS             complement(<1..200)
                     /locus_tag="VEA63_09320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein CinA"
                     /protein_id="HYD84243.1"
                     /translation="MVSSVHAGHSSKPISQVHYELLTLGDELLLGLTTNSHLAFIGQQ
                     LGRRGVTLRRNVTITDDAEAIAR"
     gene            237..>1003
                     /locus_tag="VEA63_09325"
     CDS             237..>1003
                     /locus_tag="VEA63_09325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HYD84244.1"
                     /translation="MPAIDPVNLKEKVRHLPDRPGVYLMKDRLGRIIYVGKARSLKKR
                     VSSYFQQGRARMVMQPKIRALIPLISDFETIEVKSEPEALLLEGKLIKQWRPRYNTDF
                     TDDKRFLLVRLDPHEELPRFRLTRIKKDDRSRYFGPFAHSGLLRKTLAQMRRQFGILL
                     ADGTPEKLPDGTWRLYDDVRQEIYGGLTTVTTEAYRRRVEDAASFLEGKSREWLETLR
                     AEMASAAANQAFEKAAELRDIVFALEETLRKTRRFERL"
BASE COUNT          218 a          318 c          291 g          176 t
ORIGIN      
        1 cgtgcaatcg cctcggcgtc gtcggtgatc gtgacgttgc gtcgcaacgt cacgccgcgc
       61 cgccccaatt gctgcccgat aaacgccaga tgactgttcg tcgtcagccc caaaagcagc
      121 tcatctccga gcgtcagcag ctcgtaatga acctggctga tcggcttgga agagtgtccg
      181 gcgtgaacag atgaaaccat acttgcgagg tccgagatta gacggaatct aggaaaatgc
      241 cagccatcga cccggtaaat ctcaaggaga aggtgcggca tttgcccgac cgccccgggg
      301 tgtatttgat gaaagaccgg ctcggaagaa tcatttacgt tggcaaggcg cgcagcctga
      361 aaaaacgcgt ttcatcctat tttcaacaag gccgcgcgcg catggtgatg cagccgaaaa
      421 tccgcgcgtt gattccgctg atcagcgatt tcgaaacgat cgaagtgaag tccgagcccg
      481 aggcgctgct gctcgagggc aagctgatca agcagtggcg accgcgctac aacaccgact
      541 tcaccgacga caaacgcttc ctcctcgtcc gcctcgatcc gcacgaggag ctgccgcgtt
      601 ttcgtctcac gcgcatcaag aaagacgatc gttcgcgcta cttcgggccg ttcgcgcaca
      661 gcggcctgct gcgcaaaacg ctcgcgcaga tgcgccgcca gttcggcatt ctcctggccg
      721 atggcacgcc ggaaaaactg cccgacggca cgtggcgact ctacgacgac gtgcgccagg
      781 aaatttacgg cgggctcacc accgtcacga ccgaggcgta tcgccgccgc gtcgaggacg
      841 ccgcctcttt tctcgaaggc aaatcgcgcg agtggctcga aacgcttcgc gccgagatgg
      901 ccagcgccgc cgcgaaccag gcgttcgaaa aagccgcgga gctgcgcgac atcgtgttcg
      961 ctctcgaaga aacgctgcgc aagacccgtc gcttcgaacg gct
//
LOCUS       DASPLT010000897         2629 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_34424, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000897 DASPLT010000000
VERSION     DASPLT010000897.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 2629)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2629)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2629
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_34424"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1851
                     /locus_tag="VEA63_09330"
     CDS             <1..1851
                     /locus_tag="VEA63_09330"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HYD84245.1"
                     /translation="PLVHPSSFFFAFASLVLASVSFAAQRPNILWIIAEDASPHIGPY
                     GERLIDTPAFDRLAREGITFTQAFTTAPVCSPSRSALISGMMQTTLGSHNHESNLATL
                     EVGGDASFYDSYRTPAEIKLIPELFADAGYYVVNGGTAKTHYNFVPRTELYVGSDWSG
                     RVAGQPFFAQIQLQGGKGDGITEAGATDHAAITLPPYLPNDPVLRKDWAKYLDNWKAT
                     DREVGAILARLEQEGILEHTAVFFLTDHGISHLRAKQFLYEEGLQIPLLVRLPGGGGA
                     NTRRIDLVSHLDIAASSLALAGIPIPGYVEGRALFSPGHQPRVALVGARDRCDETVDL
                     IRSVRTARFRYIRNFMSYRPHLQPNQYKDGKPIIRTLRRLHAAGRLTELQDRLFFPTR
                     PTEELYDLENDPDELTNLAGVRAFDSVRAELRAQLYQTLVDQRDCGLIPEPILDEMAR
                     AAGSKYAVLLQAEHHDLVRAIIDTIEAGERNDVETLRARLASPMPALRYWAAVWLGQL
                     RDQAARPLLVRRLHDESGAVRVAAAESLYMLGDRSPALNALKLALGDGNHNTAFYAAR
                     AWEAIGAPVDELFPFIRRSPHAHYEPIKRVVRRLAPPKRPKSTPGSHDKT"
     gene            complement(1884..>2629)
                     /locus_tag="VEA63_09335"
     CDS             complement(1884..>2629)
                     /locus_tag="VEA63_09335"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HYD84246.1"
                     /translation="VWLISSTRFAVGLVLIALVYRRECEPSHLFRNARLASRGLIGGL
                     GVYGYYVTVVHLGAGRATFINNTYVIFGALLAVWLLRERFSLAIAAGSAAALTGLALL
                     TNAFAAGSQTSAYDLLAIAVALGSAFIVVTIRQLHATEHTSTIFAAQCVYGLLICGIP
                     SMLHPAPVPALAWIIMLLAGLLAAAGQLAMTRAFRDLPVGEGSLLQMLVPIGIACGGF
                     LFFDERFARHELIGAALILAGTTFTSLRR"
BASE COUNT          503 a          825 c          843 g          458 t
ORIGIN      
        1 ccgctcgtgc accccagctc atttttcttc gcctttgcca gcttggtgct ggcttccgtc
       61 tcgttcgccg ctcaacggcc aaatattctg tggatcattg ccgaggacgc gtcgccgcac
      121 atcggtccct atggggaacg gttgatcgac acgccggcat tcgaccgtct ggcgcgtgag
      181 gggatcacct tcacccaagc tttcaccacc gcaccggtct gctccccgag ccgatccgcg
      241 ctgatctccg ggatgatgca gaccacgctc ggtagtcaca accatgagag caatctcgcc
      301 actcttgaag taggtggcga cgcgtcgttt tacgacagct accggactcc ggcggagatc
      361 aagctcatcc ccgagctgtt cgcggatgcg gggtattacg ttgtgaacgg cggcactgcg
      421 aaaacccatt acaacttcgt tccgcgcacc gagctatatg tcggcagcga ttggtcgggc
      481 cgcgtggccg gtcagccgtt tttcgcccag atccaattgc agggcgggaa gggcgatggc
      541 atcacggaag ccggagccac cgatcacgcg gccatcacgc tcccgcctta cttgccgaat
      601 gatccggtgc tgcggaaaga ctgggcgaag tatctcgata actggaaagc caccgatcgc
      661 gaagtcggcg cgatcctcgc tcgccttgaa caggaaggca tcctcgagca caccgccgtt
      721 ttctttctca ccgatcacgg gatcagccat ttgcgggcca aacaatttct ctacgaggaa
      781 ggattgcaga ttcctctgct ggtgcgcctg cccggaggcg gcggcgccaa cacgcgacgg
      841 atcgacctcg tttctcatct cgacattgcg gcctcctccc tcgccctcgc cggaattccg
      901 atcccgggct atgttgaagg ccgcgcgctg ttcagccccg gccatcagcc gcgcgtcgcg
      961 ctggtcggtg cgcgcgatcg ctgcgacgaa accgtcgatc tcatccgcag cgtgcgcacc
     1021 gcgcgtttcc gctacatccg gaatttcatg tcctaccggc cgcatctaca gccgaaccaa
     1081 tacaaggatg gaaaacccat tattcggacc ctgcgccggc ttcacgccgc cggccggctg
     1141 accgaactgc aagaccggct gttttttccc acccgcccga ctgaggagct ctacgacttg
     1201 gaaaacgatc ccgacgagct gacgaatttg gcgggcgttc gcgccttcga ttcagtgcgt
     1261 gccgaactgc gcgctcaact ctatcaaacg ctcgtcgatc agcgcgattg cggtctgatc
     1321 cccgaaccca tcctcgacga gatggcgcgc gcggccggca gcaaatatgc cgtgcttctc
     1381 caagcggaac accacgatct cgtccgcgcc atcatcgaca cgattgaagc cggcgaacgg
     1441 aacgacgtgg agacgttgcg cgcccgtctc gcgtccccga tgccggcgct tcgttactgg
     1501 gcggccgtat ggctgggaca actccgcgat caggccgcta ggccgttgct cgtccgacga
     1561 ttgcacgacg agtccggagc cgttcgggtg gcggcggcgg aatcgcttta tatgctcgga
     1621 gaccgctcgc ctgcgctcaa cgcgctgaaa ctcgcgcttg gcgacggcaa ccacaacacc
     1681 gccttttatg cggcgcgcgc ttgggaagca atcggcgctc cggtcgacga actgtttccg
     1741 ttcattcgcc gaagcccgca tgcgcattac gagccgatca aacgggtagt caggcggctc
     1801 gcccctccga agcgcccgaa gtccacgcca ggatcgcacg acaaaacatg aagcagggtt
     1861 ttttggagtt acgctgaggc gtatcagcgc cgcaaggacg tgaaggtggt gccggcgagg
     1921 atgagcgcgg cgccgatcag ttcgtggcgg gcgaagcgtt cgtcgaagaa cagaaaaccg
     1981 ccgcacgcga tgccgatggg cacgagcatc tggagcagcg agccctcgcc gacgggcagg
     2041 tcgcggaagg cgcgggtcat cgcgagttgt ccggcagcgg cgaggagacc ggcgagcagc
     2101 atgataatcc aggcgagcgc cggcacgggc gccggatgca gcatcgacgg gatgccacag
     2161 atcaaaagac cgtagacgca ttgggcggcg aagatcgtgg acgtgtgttc ggtcgcgtgg
     2221 agttggcgga tggtgacgac gatgaaggcg gagccgaggg cgacggcgat ggcgagcaga
     2281 tcatacgcgc tggtctggct gccggcggca aacgcgttgg tgaggagggc gaggccggtg
     2341 agcgcggcgg cgctgccggc ggcgatcgcg aggctgaagc gttcccgcaa cagccagacg
     2401 gcgaggaggg cgccgaagat cacgtaggta ttgttgatga acgtggcgcg gccggcaccg
     2461 agatgaacca cggtgacata gtagccgtag acgccgaggc cgccgatgag gccgcgactg
     2521 gcgaggcggg cattgcggaa aaggtgcgac ggttcgcatt cgcgtcggta gacgagcgcg
     2581 atgagcacga ggccgacggc gaaacgcgtg ctgctgatca gccagacat
//
LOCUS       DASPLT010000898         2999 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_92113, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000898 DASPLT010000000
VERSION     DASPLT010000898.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 2999)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2999)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2999
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_92113"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(40..510)
                     /locus_tag="VEA63_09340"
     CDS             complement(40..510)
                     /locus_tag="VEA63_09340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD84247.1"
                     /translation="MQRDYILRLIEQLGEFLSRITQQRERHAPQEALQSVMAACERLF
                     GLEAVQIFQFTPDQHVAMLTDGEPPENARDKVLMYAALNLEAGRCYTTLNQPKLAQQS
                     FLNALRLTLQARQKFTAANWPTFAPNITDLLALLGDTPLDASTAELLAASDRAP"
     gene            complement(627..2876)
                     /locus_tag="VEA63_09345"
     CDS             complement(627..2876)
                     /locus_tag="VEA63_09345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HYD84248.1"
                     /translation="MSTPSLLSQPLDRRSFLKISSIASGGLMLAFYLRSGSDLLGADS
                     IVNVSTDVPTGDFIPGAYIRISPTGVVTLVSKNPECGQGIKTALPMVVAEELEVNWKD
                     VVIEQADFTPAYSSPWYGAGGSTSTPNHYDLFRRAGATARTMLITAAAQTWNVPESEC
                     HADSSAIHHRTSGRKLAYSELVAKAAQLPVPDEKSVQLKDPKDYKLLGKRISGVDNPK
                     IVTGQHLFGIDTKLPGMLYAVYEKCPVFGGKPTNANLARIKTLRGVRDAFIIEGTNNI
                     TGLMPGVAIVADSTWAALSARQQLRVTWDEGKTASESWLDFTAKAEEIANQPGAQMLR
                     NDGDVDAAFAADGVKIVEAAYSYPFISHANLEPQNCTAWFKDGGVEIWAPTQNPGSGV
                     DLVSKTLNVPADKITMHVTRMGGGFGRRLTSDFVVEAAAIAQRVNAPIKLTWTREDDM
                     RHDHYRPGGFHFFKGAVDGNGKLVAWRNHFVTFGNTKERAGSGGSLNRDEFPGRWLAN
                     FRGEQTILETGVPMGPWRAPGSCVFSWVIHSFIDELAHAANRDRVEFLMELLGDNDVV
                     PGSGERSQPYNVARMRGVLKAVAEKGGWGKKLPRGQGQGIAFHFSHRGYVAQLAEVTV
                     SQDGALKVDKVVCVADVGSQIVNPSGAENQSEGSIVDGLSAMMYQEIDIDRGRVVQGN
                     FNDYPLLRMPDTPATIEIHFLKTDNPPTGVGEPVIPPTAPAIANAIFAATGHRVRQFP
                     LTKTDLSWS"
BASE COUNT          518 a          844 c         1053 g          584 t
ORIGIN      
        1 tagcggaggg ctgccgcgat ggtgcggttc aactcggcat cacggcgcgc ggtcgctggc
       61 agccaagagc tcagcggtcg aggcgtcgag cggagtgtcg ccgagcagag cgagcaaatc
      121 cgtgatgttc ggggcgaacg taggccagtt cgcggcggtg aatttctgtc gcgcctgaag
      181 cgtgagacga agggcgttga gaaacgattg ctgcgcgagc tttggctggt tgagcgttgt
      241 gtagcagcga ccggcttcga ggttcagcgc ggcatacatg agcactttgt cgcgggcgtt
      301 ttccggcggc tcgccatccg tgagcatcgc gacgtgctgg tcgggcgtga actgaaagat
      361 ttgcacggcc tcgaggccga agaggcgttc gcacgctgcc atgaccgact gcagcgcttc
      421 ctgcggcgcg tggcgttcgc gttgctgagt gatgcgcgag aggaattcgc cgagttgctc
      481 aatgaggcgg agaatgtaat cgcgctgcat ggccggaacg tggcgcgaaa cggagggcgg
      541 tcaacgcgcg gagagcggac gggcgcgtgg aaaaaagaag gaccggtcgg agaccggccc
      601 tacggtggac gctttggcgc tcgcgatcag gaccaactga gatccgtctt cgtcagcggg
      661 aattgacgca cgcgatgacc ggtcgcggcg aagatcgcgt tggcgattgc gggcgcggtc
      721 ggagggatca cgggttcgcc gacgccggtc ggcgggttgt cggttttcaa gaaatgaatc
      781 tcgatcgtcg caggcgtgtc gggcatgcgc agcagcggat agtcgttgaa attgccctgc
      841 acgacgcggc cgcggtcgat gtcgatttcc tgatacatca tcgcgctgag gccatcgacg
      901 atcgaacctt ccgactggtt ttcggcgccg ctcggattga cgatttgtga acccacgtcc
      961 gcgacgcaca caaccttgtc gactttgagc gcgccgtctt gggagacggt gacctccgcc
     1021 agttgcgcga cgtagccgcg atggctgaag tgaaacgcga tgccctggcc ctggccgcgc
     1081 ggaagctttt tgccccagcc gcctttttcg gcgacggctt tcaacacgcc gcgcatgcgg
     1141 gccacgttgt agggctgact gcgctcgccg ctgccgggaa cgacgtcgtt atcgccgaga
     1201 agctccatca ggaattcgac gcggtcgcga ttcgccgcat gtgcgagttc gtcgataaag
     1261 ctgtgaatca cccacgagaa cacgcagctg ccgggcgcgc gccacgggcc catcggcacg
     1321 ccggtttcga ggatggtttg ttcgccgcgg aaattcgcga gccagcggcc gggaaactcg
     1381 tcgcggttga gcgaaccgcc gctgcccgcg cgttccttcg tgttgccgaa ggtgacgaag
     1441 tgattgcgcc acgccacgag ctttccgttt ccgtcgaccg ctcctttgaa aaaatgaaat
     1501 ccgccggggc gataatgatc gtggcgcatg tcgtcttcgc gcgtccacgt gagcttgatc
     1561 ggagcgttca cccgctgcgc gatggcggcg gcttcgacga cgaaatcgct ggtgagccgg
     1621 cggccgaaac cgccgcccat gcgtgtgacg tgcatcgtga ttttatcggc gggcacgtta
     1681 agcgtcttgc tgaccagatc gacgccggag ccagggttct gcgtcggcgc ccagatttcg
     1741 acgccgccgt ccttgaacca agcggtgcaa ttctgcggtt cgagattcgc gtgcgagatg
     1801 aacggataac tgtaagcggc ttcgacgatc ttcacgccgt cggcggcaaa cgccgcatca
     1861 acgtcgccgt cgttgcgcag catttgcgcg ccgggctgat tggcgatttc ctcggctttc
     1921 gcggtgaaat cgagccagct ctcgctcgcg gttttgcctt cgtcccacgt cacgcgaagc
     1981 tgctggcggg cgctgagcgc ggcccaagtg gaatcggcga cgatcgcgac gcccggcatg
     2041 aggccggtga tgttgttcgt gccttcgatg atgaaagcgt cgcgcacgcc gcgcagcgtt
     2101 ttgatgcgag cgagatttgc gttggtcggc ttgccgccga agaccgggca tttctcgtaa
     2161 acggcgtaga gcatgccggg cagcttcgta tcgatgccga aaaggtgctg gccggtgacg
     2221 atcttgggat tgtcgacgcc ggagatgcgt ttcccgagga gcttgtagtc tttgggatct
     2281 ttcaactgca ccgacttttc gtcgggaacg ggaagttgcg cggctttcgc gacgagctcg
     2341 ctgtaggcaa gcttgcggcc ggaggtgcgg tggtgaatcg cgctggagtc ggcgtggcat
     2401 tcgctttcgg gaacgttcca cgtttgcgcg gcggcggtga tcaacatcgt gcgcgcggtg
     2461 gcgccggcac ggcggaaaag atcgtagtgg ttgggcgtcg aagtgctgcc gccggcgcca
     2521 taccaagggc tgctgtaggc cggcgtgaaa tccgcctgct cgatgacgac gtctttccag
     2581 ttcacctcga gctcttcggc gacgaccatc gggagagcgg tcttgatgcc ctggccgcac
     2641 tcgggatttt tcgaaacgag cgtgacgacg ccggtcggcg agatgcggat gtaagcgccg
     2701 gggataaaat cgcccgtggg cacatcggtg gaaacgttga cgatcgagtc ggcgccgagc
     2761 aggtcgctgc cggagcgaag atagaacgcg agcatcaagc cgccgctcgc gatgctcgaa
     2821 attttgagga agctgcggcg gtcgagcggt tgggagagaa gggaaggcgt gctcatgagg
     2881 cgacctccgt ggcggtgccg ttggcgatgg cggcggcgcg ttgaatgccc tggcggatac
     2941 gataatacgt ggcgcaacga cagagattgc cggccatggc gtcgtcgatg tcggtcgcg
//
LOCUS       DASPLT010000899         1862 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_134343, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000899 DASPLT010000000
VERSION     DASPLT010000899.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1862)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1862)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1862
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_134343"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..634)
                     /locus_tag="VEA63_09350"
     CDS             complement(<1..634)
                     /locus_tag="VEA63_09350"
                     /inference="COORDINATES: protein motif:HMM:NF012925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 16 protein"
                     /protein_id="HYD84249.1"
                     /translation="MISFASTPVWQDEFNQPVGSAPDPTKWVYDLGTTGWGNKELQTY
                     TDARENSFIVEDAAATDGRALVIKAVRTGADRYTSARLKTHGKFATGPARIEARLKLP
                     KGQGIWPACWMLGDNIEQVPWPVCGEIDIMEMVGHQPGTLHGTLHGPGYSADKGVTKS
                     IVLPEGATFADAYHVFAVEWKPERIDWLLDGEVYHSVTPASLPDGAKWVFD"
     gene            complement(730..>1862)
                     /locus_tag="VEA63_09355"
     CDS             complement(730..>1862)
                     /locus_tag="VEA63_09355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015486 -
                     glycoside-pentoside-hexuronide:cation symporter activity
                     [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide (GPH):cation
                     symporter"
                     /protein_id="HYD84250.1"
                     /translation="GKFRPYILWGAIPYGILGWVMFANPDLGVQGKLIYAYVTYTLVL
                     IAYVAINTPYGALMGVMSASSEDRTSLSSYRFACAFLGALLIGWLVPILKDFLTGVGD
                     SPADGFRNTMAIFAVLSVAMFIYTFANTKERVVAPPNQRSSLGRDVRDLFSNVPWLVL
                     FFVALLNLTNTGLRNGAGIYYFKYCVGQESAIATFNFVGFLCFIAGALSTKLFTARFS
                     RRSLMIVLTLINGAGIAACYFVDPQNLPLLYALNIVASFAAGPTPAIVWSLYADSADF
                     GEWKFGRRATGLLFSATVFVQKVGLALGTAMLGWMLGYYGFVANAAQSPAVMHGIVLM
                     FSLIPGCLALLAGLVTILYPLDEPQVKRMERELAARKTPVPA"
BASE COUNT          380 a          595 c          577 g          310 t
ORIGIN      
        1 cgtcgaacac ccatttcgct ccatcgggca ggctcgctgg cgtgacgctg tgatagactt
       61 cgccgtcgag cagccagtcg atgcgctccg gtttccactc caccgcgaac acgtgatacg
      121 catcggcgaa cgtcgcgccc tccggcagca cgattgattt tgtcacacct ttgtccgccg
      181 aataacccgg gccgtgcaac gtcccatgga gcgtgcccgg ctggtgcccc accatctcca
      241 tgatgtcgat ctcgccgcac acgggccacg gcacttgctc aatgttgtcg ccgagcatcc
      301 agcacgcggg ccagatcccc tgccctttcg gcagcttcaa acgcgcctcg atccgcgcgg
      361 gaccggtggc gaattttccg tgcgtcttca aacgcgccga cgtgtagcgg tcggcgcccg
      421 tgcgcaccgc tttgatcacg agcgcgcggc cgtccgtcgc tgcggcgtcc tcaacgatga
      481 acgagttctc ccgcgcatcg gtgtaggttt gcagctcctt gttgccccaa cccgtggtgc
      541 cgagatcgta aacccacttc gtcggatcgg gggcgctgcc taccggctgg ttgaactcgt
      601 cctgccagac cggagtggat gcgaaggaaa tcatggggat aaaaagcgaa acaatgcggc
      661 gaaggcggag cttcacataa gtttgggata cgactgcgcc gcgagcggcg gccgatgccg
      721 gcgcaccgct catgcgggca ccggcgtttt ccgcgcggcg agttcgcgct ccatgcgctt
      781 cacctgcggc tcgtcgagcg gatagagaat ggtcacgagc cccgcgagca gcgccaggca
      841 gccgggaatc aggctaaaca tcagcacgat gccgtgcatc accgccggcg attgcgcggc
      901 gttcgccacg aagccgtaat agccgagcat ccaaccgagc atcgcggtgc cgagcgcgag
      961 gccgactttc tgcacaaaca ccgtcgcgga aaataacagc ccggtcgcgc gacggccgaa
     1021 cttccattcg ccgaagtccg cgctgtcggc gtagagcgac catacgatcg cgggcgtggg
     1081 gccggcggcg aacgacgcca cgatgttgag cgcgtaaagc agcgggaggt tttgcggatc
     1141 gacgaaataa cacgccgcga tgcccgcgcc gttgatcagc gtgagcacga tcatcaacga
     1201 ccgccgcgag aagcgcgcgg tgaagagttt cgtcgaaagc gcgcccgcga tgaagcagag
     1261 aaaaccgacg aagttgaacg tcgcgatcgc cgactcctgc ccgacgcagt atttgaaata
     1321 gtaaatcccc gcgccgttgc gcagccccgt gttggtgagg ttcagcagcg cgacgaaaaa
     1381 cagcacgagc cacggcacgt tcgagaacag gtcgcgcaca tcgcgtccga gcgagctgcg
     1441 ttgattcggc ggtgcgacga cgcgctcctt cgtgttggcg aaggtgtaga tgaacatcgc
     1501 taccgacagc accgcgaaga tcgccatcgt gtttctaaat ccgtccgccg gcgaatcgcc
     1561 gacgccggtg aggaagtctt tcaggatcgg cacgagccag ccgatcaaca gcgcgccgag
     1621 aaacgcgcac gcgaatcgat acgacgagag cgaggtgcgg tcttccgacg acgccgacat
     1681 cacccccatc agcgcaccgt aaggcgtgtt gattgccacg taggcgatga gcacgagcgt
     1741 gtaggtgacg taggcgtaga tgagtttgcc ctgaacgccg agatcgggat tcgcgaacat
     1801 cacccagccg agaatgccgt agggaatcgc gccccacaga atgtaaggcc gaaattttcc
     1861 cc
//
LOCUS       DASPLT010000900         1273 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_92154, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000900 DASPLT010000000
VERSION     DASPLT010000900.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1273)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1273)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1273
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_92154"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..830
                     /locus_tag="VEA63_09360"
     CDS             <1..830
                     /locus_tag="VEA63_09360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="nucleoside transporter C-terminal
                     domain-containing protein"
                     /protein_id="HYD84251.1"
                     /translation="LLIKPYLPTMTRSEMLAVMVGGMATIAGGVMIAYISFLGGNDPA
                     QQILFATHLITASVISAPAALTVSKILLPQVETVDRDLSVPKEKVGANLLDAICLGTT
                     DGLRLAVNIGGMLIVFIALVALCNYILSDWIGAPIGLNTWVVSVTGGTYQTFSLEFLL
                     GVACAPIAWLIGIDSGHLMVSGQLLGTRTVLNEFVAYLQLGQLKAAGQFTDERSLIIL
                     TYALCGFANIVSIGIQVGGIGALAPNQRTTLARLGVKALIGASLACFLTACVAGMMT"
     gene            complement(1012..>1273)
                     /locus_tag="VEA63_09365"
     CDS             complement(1012..>1273)
                     /locus_tag="VEA63_09365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YdbL family protein"
                     /protein_id="HYD84252.1"
                     /translation="GQIDQLKSSGAVGENNRGFLEVRGGGGDASSVVRAENADRETVY
                     AAIAKQTGSSAEQVGRARARQIAAGSAPGVWLQREDGNWYKK"
BASE COUNT          200 a          505 c          322 g          246 t
ORIGIN      
        1 cgctcctcat caagccctac ctcccgacca tgacacgctc cgagatgctc gccgtcatgg
       61 tgggcggcat ggccacgatc gccggcggcg tgatgatcgc ctacatcagc ttcctcggcg
      121 gcaacgatcc cgctcaacaa atccttttcg ccacgcacct catcaccgcg tccgtcatct
      181 ccgcgcccgc cgcgctcacg gtatccaaaa tccttctacc gcaggtggag accgtcgacc
      241 gtgacctgtc cgtgccgaag gaaaaagtcg gcgccaatct cctcgacgcc atttgcctcg
      301 gcacgaccga cggcctacgg ctcgccgtca acatcggcgg tatgttgatc gtgttcatcg
      361 cgctcgtcgc cctctgcaac tacatcctca gcgattggat cggtgcgccg atcggactga
      421 acacctgggt cgtctccgtc accggcggca cctaccagac gttctcgctc gaattcctcc
      481 tcggtgttgc ctgcgcgccg atcgcctggc tgatcggtat cgacagcgga catctcatgg
      541 tgtccggcca gcttctcggc acgcgcaccg tgctgaacga attcgtcgcc tacctccaac
      601 tcggtcagct caaagccgcc ggtcagttca ccgacgaacg ctcgctcatc atcctcactt
      661 acgcgctttg cggtttcgcc aacatcgtct ccattggcat ccaagtcggc ggcatcggcg
      721 cgctcgcgcc caatcaacgc accaccctgg cgcgactcgg cgtcaaggcg ctcatcggcg
      781 ccagcctagc gtgcttcctc accgcctgcg tcgccggcat gatgacgtga cgcgtcggca
      841 acaacgcccc acgtagccgc acgcctcggc gtgcggaaat cgcgcctccc gcgtcctcac
      901 ctttccgacg tagccacacg ccttcgcgtg cgggcgccct gtcgcacgag cgactctcgc
      961 gcgccccacc gcgccgttcc tcgtgtcacc gctcgcccgc gctctccgcg cctacttctt
     1021 ataccaattc ccatcctcgc gctgcagcca aactcccggc gcacttcccg ccgcgatctg
     1081 ccgcgcccgc gcccggccaa cttgctcggc cgacgagcct gtctgtttcg cgatcgccgc
     1141 atagaccgtc tcccggtccg cattttccgc gcggaccacg ctcgacgcat cgccgccgcc
     1201 tccgcgcact tccagaaaac cacgattgtt ttcgcctacc gcaccggacg atttgagctg
     1261 atcgatttgc ccg
//
LOCUS       DASPLT010000901         1533 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_71105, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000901 DASPLT010000000
VERSION     DASPLT010000901.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1533)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1533)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1533
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_71105"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1533)
                     /locus_tag="VEA63_09370"
     CDS             complement(<1..>1533)
                     /locus_tag="VEA63_09370"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HYD84253.1"
                     /translation="ALDLSLQKSDSDLIGNGALPVGMLGSDRAAVFTAPDITANDVEM
                     FDFSGNHRFSDTLAFSGNVFRRENTTDSFNGDGSEFELCNFAGGAQALFEEDDGLEDA
                     LEDDLDIELDEICEGEEDDIRSFADLEELIEDAAEAAGLDDDMYEPEDISGELSGTGV
                     LSDVAINNISRRVQESSGFGGQFAFTQDLFARPNQFIAGYAWFRGKSTFESVLELAEL
                     DPVTRSTQGLGTGAFIIDAETDIRTETETRSIYFSNTIDLNAELALTLSGRYNDTDVT
                     LRDRSGERPELNGDHNFTRFNPAVGLTWTPDAELTVYGSYSESNRVPTPIELACNEGV
                     FELARQYAIEEGEDPDDIEFECRLPNAFLADPPLDDVVTSSIEFGTRMVVNGVRYSVG
                     VFHATNENDILFQTTGRSTGLFANVDETQRRGVEFAANGSFAQLDWYASYSHIRATFE
                     DDFAVLSPNHPGANADGELDVQAGDRMPGIPEDLFKLGADYHFMPNATAGVEVVHNGS
                     QV"
BASE COUNT          264 a          473 c          476 g          320 t
ORIGIN      
        1 cgacctggct gccgttgtgc accacttcga cgccggcagt agcgtttggc atgaagtgat
       61 aatccgcgcc gagcttgaac aggtcttccg gaatgcccgg catgcggtcg ccggcctgca
      121 catcgagttc gccatcggca ttcgcgcccg gatgattcgg gctgagcacc gcaaagtcgt
      181 cttcaaacgt ggcgcgaatg tgcgaatacg acgcatacca gtcgagctgg gcgaagctgc
      241 cattggcggc gaattccacg ccgcggcgct gcgtttcatc gacgttcgca aacaggccgg
      301 tggaccgacc ggtggtctgg aacaggatgt cgttctcgtt cgtcgcgtgg aacacgccaa
      361 ccgaatagcg cacgccgttc accaccatgc gcgtgccgaa ctcgatgctg ctcgtgacga
      421 cgtcatcgag cggcgggtcg gcaagaaatg cgttcggcag gcggcattcg aattcgatgt
      481 cgtcggggtc ttcgccctct tcgatcgcgt actggcgcgc aagctcgaac acgccttcgt
      541 tgcacgcgag ttcgatcggc gtcggcacgc gattcgattc actgtacgaa ccgtacacgg
      601 tgagctcggc atcgggagtc caggtaagac cgaccgccgg gttgaaacgc gtgaagttgt
      661 gatcgccatt cagttccggc cgttcgccgg agcggtcgcg cagcgtcacg tcggtgtcgt
      721 tgtagcggcc cgacaacgtc agcgcgagtt ccgcgttcag gtcgatcgta ttgctgaaat
      781 agatgctgcg cgtttcggtt tcggtgcgga tgtcggtttc ggcatcaatg atgaacgcgc
      841 cggtgccaag gccctgcgtg ctgcgtgtca ccggatcgag ctcggcgagt tccagtaccg
      901 attcgaacgt cgacttgccg cggaaccacg cgtaacccgc gatgaactgg ttcgggcgcg
      961 cgaacagatc ctgcgtgaac gcgaactggc cgccgaagcc gctgctttcc tgcacgcgtc
     1021 ggctgatgtt gttgatcgcc acgtcgctga gcacgcccgt gccgctcagt tcgccgctga
     1081 tatcttcggg ttcgtacata tcgtcatcga gacccgccgc ttcggccgca tcctcgatca
     1141 gttcctcgag atcggcgaaa ctgcggatgt cgtcttcttc accttcgcag atctcgtcga
     1201 gttcgatgtc gagatcgtct tcgagcgcat cttcgagacc gtcgtcttcc tcgaacagcg
     1261 cctgcgcgcc gccggcgaag ttgcacagtt cgaattcgga gccatcgccg ttgaacgaat
     1321 ccgtcgtgtt ttcgcggcgg aacacgttgc cgctgaaggc cagcgtgtcg ctgaaacggt
     1381 gattgccgct gaagtcgaac atctcgacgt cgttcgcggt gatgtcagga gccgtgaaca
     1441 ccgccgcgcg atcgctaccc agcatgccca ccggcagcgc gccgttgccg atcaggtcgc
     1501 tgtcactttt ctgcaggctc aggtcgagcg cgg
//
LOCUS       DASPLT010000902         1801 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_112340, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000902 DASPLT010000000
VERSION     DASPLT010000902.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1801)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1801)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1801
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_112340"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1801
                     /gene="pheT"
                     /locus_tag="VEA63_09375"
     CDS             <1..>1801
                     /gene="pheT"
                     /locus_tag="VEA63_09375"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HYD84254.1"
                     /translation="LSQVVVGEVLVRDKHPNADKLSVCQVDIGSAGGVKTIVCGAQNY
                     KVGDRVPVALPGAILPGNFEIKQSKIRGQSSDGMMCSAKELGVAEDAAGLLILDGRPE
                     LGTPINAVLPPGDTVFDIEITPNRPDCLSHLGIARELAAWFKLPLLYPQEKFRGAVGD
                     ASRPDLLKNVTVLSPEACPLYTAHVITGIKIGPSPAWMQQRLAAVGLRPINNVVDVGN
                     YVMLETGQPLHAFDARKLAGNEIIVRPAADGEKITTLDGKERVLNSRMLVIADAQKPV
                     VIAGIMGGENSGVADDTTDLVLECAIFKRQSVRWTSRKLGLSSDSSYRYERGVDPHGA
                     LEAAWRAVDLIVETAGGQVVGPVFKVGGDVPWQREIVVTHDYITEKLGFDIPAEEMRA
                     AFDALELTVTREEPTERRGPAWTVSVPSWRDDLDRPIDLVEEVLRLYGTEKIPAAVVL
                     SPGLARDDDPIVGFNRRVTDYLVGHDFHECVNYTLRPAKELATWVSQTAAAELALANP
                     FVEDQSHLRPTLITGLLDALKLNQSRGVAVSRLFETGRIFIERNGQNFECAAVGFIIA
                     EDAERRWKQREPADFYAAKHHVIALAESAGIDLA"
BASE COUNT          326 a          585 c          563 g          327 t
ORIGIN      
        1 ctcagtcagg tcgtcgtcgg cgaagtgctc gtgcgcgaca agcatccgaa cgccgacaaa
       61 ctttccgtct gccaggtcga catcggctcg gcgggcggcg tgaagacgat cgtctgcggc
      121 gcgcagaatt acaaagtcgg cgaccgcgtg cccgttgcgc tgcccggcgc gattcttccc
      181 ggtaatttcg aaatcaagca gtcgaagatc cgcggtcagt cctccgacgg catgatgtgt
      241 tcggcgaagg aactcggcgt cgccgaagac gccgccggtc tcctcatcct cgacggacgt
      301 cccgaactcg gcacgccgat caacgccgtg ctgccgcccg gcgacaccgt gttcgacatc
      361 gagatcacgc cgaaccgccc cgattgtctt tcgcacctcg gcatcgcgcg cgagctcgcc
      421 gcgtggttca agctcccgct gctttatccc caggaaaaat tccgcggggc cgtcggcgat
      481 gcatcgcgtc ccgacttgtt gaagaacgtg accgttcttt ctccggaagc ttgtccgctc
      541 tacaccgcgc acgtcatcac cgggatcaag atcggcccca gccccgcgtg gatgcagcaa
      601 cgtctcgcag cggtcggact tcgcccgatt aacaacgtcg tcgacgtcgg caactacgtg
      661 atgctcgaga ccggccagcc gctgcacgcg ttcgacgcgc gtaagctcgc gggcaacgaa
      721 atcatcgttc gccccgcggc cgacggcgaa aagatcacga cgctcgatgg caaggaacgc
      781 gtgctcaaca gccgcatgct cgtcattgcc gatgcgcaaa aaccggtcgt catcgcaggt
      841 atcatgggcg gcgagaattc cggcgttgcc gacgacacca cggacctcgt gctcgaatgc
      901 gcgattttta agcgacagtc cgtgcgctgg acgtcgcgca aacttggtct ttcgtccgac
      961 tcgtcctatc gctacgaacg cggcgtcgac ccgcacggcg ctctggaagc tgcctggcgg
     1021 gcggttgacc tcatcgttga gacggccgga ggacaggtcg tcgggccagt tttcaaggtc
     1081 ggcggcgatg tgccgtggca gcgcgagatc gtcgtgacgc acgattacat caccgaaaaa
     1141 ctcggtttcg atattcccgc cgaggagatg cgggccgcgt ttgacgcgct tgagctcacc
     1201 gtcactcgcg aagaaccgac cgagcgtcgc ggcccggcgt ggacggtcag cgtgccgagc
     1261 tggcgcgatg acctcgaccg gccgatcgac ctcgtcgagg aagtgttgcg actctacggc
     1321 acggaaaaaa ttcccgcggc ggtcgtgctg tcgccgggcc tcgctcgtga tgacgatccg
     1381 atcgtcggct tcaaccgtcg cgtgacggac tatctcgtcg gccacgattt ccacgagtgc
     1441 gtgaactaca cgctgcggcc ggcgaaggaa ctcgcgacct gggtgtcgca aaccgcggcg
     1501 gccgaactcg cgttggcgaa cccgttcgtc gaggaccagt cgcatctccg accgaccttg
     1561 atcacgggat tgctcgacgc gttgaagttg aatcaatcgc gcggcgtcgc cgtttcgcgg
     1621 ctgttcgaga cggggcggat tttcatcgag cgtaacggcc agaatttcga gtgcgccgcc
     1681 gtgggcttca tcattgccga ggacgcggag cgtcgctgga agcagcgcga gccggcggat
     1741 ttctatgccg caaaacacca cgtgatcgcg ctcgcggaat cggcgggcat cgatctcgcg
     1801 c
//
LOCUS       DASPLT010000903         1392 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_112364, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000903 DASPLT010000000
VERSION     DASPLT010000903.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1392)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1392)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1392
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_112364"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..605
                     /locus_tag="VEA63_09380"
     CDS             <1..605
                     /locus_tag="VEA63_09380"
                     /inference="COORDINATES: protein motif:HMM:NF024685.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SIR2 family protein"
                     /protein_id="HYD84255.1"
                     /translation="PDADYLPNTRIRAVVNFNLDAVLRAYAAARYDTYLFRTIERADK
                     PQRIGRIPIYHMHGLVKFDRSRRDDDTHEIRSVFTEGEYFDVFNQPHSVFNYTFLYLL
                     REYNCLFIGLSMNDENIRRLLHYSTSERRRQVGDSASSPRAALRHFAILRRTPSKEVD
                     ALIDISLRRLGTRVLWIANFAELPSRLAYVYGQAEWENVY"
     gene            869..1342
                     /locus_tag="VEA63_09385"
     CDS             869..1342
                     /locus_tag="VEA63_09385"
                     /inference="COORDINATES: protein motif:HMM:NF012451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HYD84256.1"
                     /translation="MPTLRTHYDTLKVSRDAPIEVIQAAYRSLARKYHPDRNRHTPEG
                     EALMKSINAAYEVLSDPSKRAEHDRWISTQLDSRRPSSPWTDSTSSRESASSEDSTSA
                     REPDVAQLSRVLLNILFSICYFSTAFWLLRNPGGRLLGLLMLVGGWLFFVARRRR"
BASE COUNT          268 a          537 c          300 g          287 t
ORIGIN      
        1 ctcccgatgc cgattacctg cccaacaccc gcattcgcgc cgtcgtcaac ttcaacctcg
       61 acgccgtgct tcgcgcctac gccgccgccc gctacgacac ctaccttttc cgaacgatcg
      121 agcgcgcgga caaaccccag cgcatcggcc gcatcccgat ctaccacatg cacggcctgg
      181 tcaaattcga ccgctcccgt cgcgacgacg acacgcacga gatccgctcc gtcttcaccg
      241 agggcgaata cttcgacgtc ttcaaccagc cgcacagcgt cttcaattac acctttctgt
      301 atctgctaag agaatacaac tgcctcttca tcggcctttc gatgaacgac gaaaacattc
      361 gccgcctcct gcattactcc accagcgagc ggcgccgaca ggtcggcgat tcggcctcga
      421 gtcctcgcgc cgcgctccgt cacttcgcga tcctccgtcg cacgccctcg aaagaagtgg
      481 acgcattgat cgacatttcc cttcgccgcc tcggcacccg cgtcctctgg atcgcgaact
      541 tcgccgaact cccctcgcgc ctcgcctacg tctacggcca ggccgagtgg gaaaatgtct
      601 actgaggcac tcgtcgcaaa agcataatct ttccactccg ccacgaatca tcccgtagcg
      661 aaaagcgtaa gcttttcgtc cggcacacaa cgccaaccca actcatctcg cagcgaacgc
      721 atcttctccc gccgcccctc gcaacgctcc aacgtttgtc atctaataga tgacaaactc
      781 tcgcggccgc ccgcatactc cgactaaaaa aatttcctcc tccctattcg cctctttccc
      841 ttctttcgtg ttctctcctt cgcgcctgat gccgaccctc cggacccact acgacaccct
      901 caaagtttcg cgcgacgccc cgatcgaagt gattcaagcc gcttaccgtt cgctcgcacg
      961 caagtatcat ccggaccgca accgccacac ccccgaaggc gaagcgctca tgaaatccat
     1021 caacgccgcc tacgaggtgt tgagcgaccc ctcgaaacgc gccgaacacg accgctggat
     1081 ttccactcaa ctcgactcgc gccggccctc atctccctgg actgactcca cgtcgtcccg
     1141 cgagtccgca tcttccgagg acagcacctc cgctcgcgaa cccgacgtcg cccagctctc
     1201 gcgcgttctc ctcaacattc ttttttcgat ctgttatttt agcaccgcct tctggctgct
     1261 gcgaaatccc ggaggacgcc tcctcggcct tctcatgctg gtcggcggct ggctcttctt
     1321 cgtcgcccgc cgccgccgct agtgtcgtgt cccgcaagta atctcagaaa agcgttcgat
     1381 cttgccgagg at
//
LOCUS       DASPLT010000904         1043 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_134394, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000904 DASPLT010000000
VERSION     DASPLT010000904.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1043)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1043)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1043
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_134394"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            290..>1043
                     /gene="menD"
                     /locus_tag="VEA63_09390"
     CDS             290..>1043
                     /gene="menD"
                     /locus_tag="VEA63_09390"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070204 -
                     2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-
                     carboxylic-acid synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HYD84257.1"
                     /translation="MKLDFRNVNALWGSVLVETLFRRGVRTAVISPGSRSTPLTMAFA
                     AHAEVEAVPVLDERSAAFFALGIAKRTKRPVVLLCTSGTAGANYFPAVIEAKESGVPL
                     LVITADRPPELRDCASGQTIDQQKLFGGYVNFYHELAVPTATLAMLGYLRQTIAHAVE
                     RTLSPVAGPVHLNAPFRDPLPPVEDGSTREAEGKIDETFFSHLDAVMPVVSHATIWQR
                     PTTTRGLIVAGPAAPENVIHYMTKLRELREKLG"
BASE COUNT          173 a          256 c          410 g          204 t
ORIGIN      
        1 tgggaaaagt ggatacgcga aggcggcgag cggacgttga tgccggcgtt tgcgcaggcg
       61 catggggggc cgttggacga tgcgcaggtg agctcgttgg tgacgtattt gacggagcat
      121 ttgtcgccga acgcggagaa gaaatgattt tcaacgacga atgcacacag atgaacgcgg
      181 atgtggagcg ctcgaaaggt gggagcctgc ttgcaggcga ttgcgcaccg atcgcgggtg
      241 aggctccgga tcgcctgcaa gcaggctcct acctttctga tctgttttca tgaagctcga
      301 ttttcggaat gtgaatgcgc tgtggggcag cgtgctggtg gagacgcttt tccggcgcgg
      361 cgtgcggacg gcggtgattt cgccggggtc gcggtcgacg ccgttgacga tggcgttcgc
      421 ggcgcatgcg gaggtcgagg cggtgccggt gctcgacgag cgctcggcgg cgtttttcgc
      481 gctcgggatc gcgaagcgga cgaagcgtcc ggtggtgctg ctgtgcacga gcgggacggc
      541 gggtgcgaat tattttccgg cggtgatcga ggcgaaggag agcggcgtgc cgttgttggt
      601 gatcacggcg gatcgtccgc cggagctgcg cgattgcgcg tcggggcaga cgatcgatca
      661 gcagaagttg tttggtggat atgtgaactt ttatcacgag ctcgcggtgc cgacggcgac
      721 gttggcgatg ctgggttatc tgcggcagac gatcgcgcat gcggtggagc gaacgctgtc
      781 gccggtcgcg gggccggtgc atctcaacgc gccgtttcgc gatccgctgc cgccggtcga
      841 ggatggaagc acgcgggagg cggaggggaa gatcgacgag actttctttt cgcatctcga
      901 cgcggtgatg ccggtggtga gtcacgcgac gatctggcag cggccgacga cgacgcgcgg
      961 gctgattgtg gcggggccgg cggcgccgga gaatgtcatc cattatatga caaagctccg
     1021 ggagctgcgg gagaagctcg ggt
//
LOCUS       DASPLT010000905         1785 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_214824, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000905 DASPLT010000000
VERSION     DASPLT010000905.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1785)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1785)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1785
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_214824"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..984
                     /locus_tag="VEA63_09395"
     CDS             <1..984
                     /locus_tag="VEA63_09395"
                     /inference="COORDINATES: protein motif:HMM:NF018190.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0016052 - carbohydrate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding protein"
                     /protein_id="HYD84258.1"
                     /translation="GKPIILSEWHFASPNEGLSSSKGVRDQRERGLAYRNYVEQAAAL
                     PFVVGSEWFIYTDQALTGRYFEGFNGEGNNTGLVNVADRPYRELVTAAAETHARIYPV
                     MFGQQRPFSFDDPRFVPHSGGGLKEVTVPRAPVGFTLDGTTRAWPQRPAEPIEPARLS
                     HGLPNEKLRADFRLCWDDAKLYLHVQVKDATPMLNHRRPRAFWMGDCVEIYLGTEKLE
                     QGGTPLFSDRHILLAASRGQAFFIDEPEFGDRCTVIASKEVSGDGYVVQLAIPWELLG
                     GAPSAGKVLLFDLGVDNSDDGYERRQQLMWNGNAKNFGDRALWGRARLISN"
     gene            1110..1193
                     /locus_tag="VEA63_09400"
     tRNA            1110..1193
                     /locus_tag="VEA63_09400"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1143..1145,aa:Leu,seq:tag)
     gene            complement(1210..>1785)
                     /locus_tag="VEA63_09405"
     CDS             complement(1210..>1785)
                     /locus_tag="VEA63_09405"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HYD84259.1"
                     /translation="CRGIPPEIETIAHQNRGYVAPDRTMILDTAPFLLLRRAPVRREC
                     TALRKNSTEESISSSGMKEPATILLVDDDEMAREVIGNLLRRAISTARVVAVEHGEAG
                     LRFYQEANPELVITDMKMVGMSGAELIQAIRETDQLTPIIVVSGAPEVVPVVGATEVF
                     QKVDFRRLIERARELIEGPAPAACASPRAPA"
BASE COUNT          322 a          515 c          612 g          336 t
ORIGIN      
        1 ggaaagccga tcatcctcag cgagtggcat ttcgcgtcgc cgaacgaagg gctgagctcg
       61 agcaagggcg tgcgtgatca gcgcgaacgc gggctcgctt atcgcaacta tgtcgagcag
      121 gcggcggcgc tgccgttcgt cgtcggcagc gaatggttta tctacaccga tcaggcgctg
      181 acgggccggt atttcgaggg cttcaacggc gagggcaaca acaccggctt ggtgaacgtc
      241 gccgaccggc cttatcgcga gctcgtcact gcggcggcgg agacgcatgc gcggatttac
      301 ccggtgatgt ttgggcaaca gcggccgttc agcttcgacg atccgcggtt cgtgccgcat
      361 tccggcggcg ggctcaagga agtgaccgtg ccacgtgcgc cggtgggttt cacgctggac
      421 ggcacgacgc gcgcgtggcc gcagcggccg gcggagccga tcgagccggc gcggctctcg
      481 cacggtttgc cgaacgagaa gctgcgcgcg gattttcggc tttgttggga cgacgctaaa
      541 ctctacctac acgtgcaggt gaaggatgcg acgccgatgt tgaaccatcg gcggccccgc
      601 gcgttttgga tgggcgactg cgtcgagatt tatctcggca cggaaaagct cgagcaaggc
      661 ggcacgccgc tgttttccga ccggcacatc ctgttggcgg cgtcgcgtgg ccaggcgttt
      721 ttcatcgacg agccggagtt cggcgatcgc tgcacggtga tcgcgagcaa agaggtttcg
      781 ggcgacggct atgtggtgca gctcgcgatt ccgtgggaat tgttgggcgg cgcgccgtca
      841 gcggggaaag tgctgctttt cgatctgggc gtggacaaca gcgacgacgg ctacgagcgg
      901 cgccagcagt tgatgtggaa tgggaacgcg aagaacttcg gcgaccgggc gctttggggc
      961 cgggcgcggc tgatttcaaa ttgaacgaga gccccgcgga agggcgtctg cgcgatgacg
     1021 tggcgcagcg aaggagcggg cgcggggcgc gggaatttcc tcgcttgcgt gacgcccgag
     1081 cgaaacgcca ctgtttgcga acgcccctcg ctcgggtggt ggaatggcag acacgacggt
     1141 cttagaagcc gttgccgtaa ggcgtgcagg ttcaagtcct gtcccgagca ccagccagac
     1201 tcagcggctt caggcgggcg cgcgcggcga tgcgcaggcg gcgggggcgg ggccttcgat
     1261 taactcgcgg gcacgctcga tgagccggcg aaagtcgact ttctggaaaa cctccgtcgc
     1321 accgaccacg ggcacgactt ccggcgctcc ggacacgacg atgatcggcg tgagctgatc
     1381 tgtttcccgg attgcttgaa taagctccgc accagacatc ccaaccatct tcatgtcggt
     1441 gatcacgagt tccggattgg cctcctgata aaaacggagg cctgcttcgc catgctccac
     1501 cgcaaccacc ctggcggtag agatggcacg acgcaggagg tttccgatca cctcccgagc
     1561 catttcgtcg tcgtcgacga ggagaattgt cgctggttct ttcatcccgg agctggaaat
     1621 gctctcttcg gtcgaattct tccgaagtgc agtgcattca cgtctgacag gtgcgcgacg
     1681 cagaagcaag aacggtgctg tatccaaaat catggttcga tccggcgcga cgtatccgcg
     1741 attctgatgc gcgatggttt cgatttcagg cgggatgcca cggca
//
LOCUS       DASPLT010000906         1256 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_214828, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000906 DASPLT010000000
VERSION     DASPLT010000906.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1256)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1256)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1256
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_214828"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..690
                     /locus_tag="VEA63_09410"
     CDS             <1..690
                     /locus_tag="VEA63_09410"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HYD84260.1"
                     /translation="AGVLASEGLVRDEVTADFNSYSFDSASDGWEVEVVANLTPNWRL
                     LANFADRTTVRANSGRELFAYFDEWRDLWLAHGALLTEGGTSVADRLAGIEGDNHTRI
                     VVPDNRAALGDPRYSANLRTNYSFRDGLLEGFSIGGGVRWRGETITAYTAAGEALDTS
                     GFALYDLNLGYSTDVTIAGKRIPLQFQLNASNLFDDQDIIPTRLYDNGSIRTFRLQEP
                     RQIYATVSVRF"
     gene            974..>1256
                     /locus_tag="VEA63_09415"
     CDS             974..>1256
                     /locus_tag="VEA63_09415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD84261.1"
                     /translation="MSSVTSPSSAPPPTGFRRTHVVWIALMYCATGLAEATSNYIMPD
                     AIHRFTQNAFFISIILSLNPLFGFVAQPLAGWYSDRIWTPLGRRLPIILA"
BASE COUNT          225 a          461 c          310 g          260 t
ORIGIN      
        1 gccggcgtcc tcgcgagcga gggtctggtc cgtgacgagg ttaccgccga tttcaactcc
       61 tacagcttcg actccgcctc cgatggttgg gaagtcgaag tcgtcgccaa cctcacgcca
      121 aactggcgcc tgctcgccaa cttcgccgat cgcaccaccg tgcgcgccaa ctccggccgc
      181 gagctcttcg cctacttcga cgaatggcgc gacctctggc tcgcccacgg tgcgcttctc
      241 accgaaggcg gcaccagtgt ggccgatcgt ctcgcgggca ttgaaggcga caaccatact
      301 cgcatcgtcg tgcccgacaa ccgggcagcc ctcggcgacc cgcgctattc cgcaaatctg
      361 cgcaccaact actcgtttcg cgatggcctc ctcgagggct tctccatcgg cggcggcgtt
      421 cgctggcgcg gcgagaccat cacggcctac accgctgccg gcgaagcgct cgatacttcc
      481 ggcttcgccc tctacgacct gaacctcggc tacagcaccg acgtcaccat cgccggaaaa
      541 cgcatcccgc tgcaatttca gttgaacgcc agcaatctgt tcgacgatca ggatatcatc
      601 cccacgcgcc tctacgacaa tggctcaatc cgcaccttcc gtctgcagga gccccgtcaa
      661 atctacgcga ccgtcagcgt tcgcttctga agatgaaacc accgataaac acagatgcac
      721 acagatgcga gcatgcccaa ccgagctaaa ccatctgatc cgtgtccatc cgtgtttatc
      781 cgtggttagg aaaatcatac ccctaacagg accggatcgg cattcgtgcc ccttcgtggt
      841 taaacgggat tgtttcggct gagcgcttcg tttcggccat catcgcatcg cgaaattttc
      901 ttcgcatcgc aaacgcccct ttgctcgctc gaagacgctc cgcgtagttc gctctcctcg
      961 ccctccaact ccgttgtcct ccgtcacctc accgagttcc gctccgccgc caaccggttt
     1021 ccgccgcacg catgtcgtgt ggatcgcctt gatgtattgc gccacgggcc tggccgaggc
     1081 cacgtccaac tacatcatgc ccgatgcgat ccatcggttc acgcagaacg cgttcttcat
     1141 ctcgatcatc ctttcgctca accccctctt cggcttcgtc gcgcaaccgc tcgccggctg
     1201 gtatagcgac cgcatctgga cgcctctcgg ccggcgcctt ccgatcattc tcgccg
//
LOCUS       DASPLT010000907         1870 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_196011, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000907 DASPLT010000000
VERSION     DASPLT010000907.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1870)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1870)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1870
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_196011"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1870
                     /locus_tag="VEA63_09420"
     CDS             <1..>1870
                     /locus_tag="VEA63_09420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD84262.1"
                     /translation="VIGGTVPKDVLIRALGPTLSQAGVSGAFSQLRLQVFDAAGVPMT
                     SNDQWSPSLKAAFAQVGASTLPDNSRDAAVRLTLAPGVYSAQISGLSEAKGIALVEVY
                     EMDGVSRLLNLSTRARVESGDGILISGLVIAGKGTRRVLIRALGPVLAAVGVAGTLAD
                     PVLSVINAGGAVVASNDNWEVANGSTNIAAIAAAAGAGALGAGSKDSALVADLPAGVY
                     TTHVRGGDGGSGVALLEVYDVSAVGELSQSAPATGGWEPGYSEAALSWAPADVSTRLG
                     PTFEELNPGAADDRPTLFQRESKMAGSKYYLRINPYELGEPGSYNDYWSDTGQVAYVP
                     DDAANDPGLDRVQLYAYYNKVFAISPRLDWGSGKPHPDPQTREPNYIKLNGGTMPMQP
                     VAMARGYGMQQNEQVMVYRDGLFAVAGMQTSRNGSERPYPGFKFPAHKVPRAIAVTTS
                     NEFALVTIWDTERTQGQLAVVALEAKFLPFHTWPYMGLPNQGSFSDVKLLGYIDLPMK
                     SPNAVSAASNGLWLGPSSTAGKVLSQIDLADDASRQLVYDGAWQSVVAKSGYAIVSST
                     EDNKAVIVDLTPLFSYMRESYLGSSESFQRTIASRGPAPEQFPQTFAVNPASMPRVI"
BASE COUNT          324 a          525 c          683 g          338 t
ORIGIN      
        1 gtgatcggcg gcacggttcc gaaggacgtg ttgatccgcg cgctcgggcc gacgttgagc
       61 caggcggggg tcagcggtgc gttctcgcag ttgcggctgc aggtttttga cgcggcgggt
      121 gttccgatga cctcgaacga ccaatggtcg ccgtcgctca aagcggcgtt cgctcaggtg
      181 ggcgcgtcga ctttaccgga caacagtcgc gatgcggcgg tgcggttgac gctggcgcca
      241 ggcgtttaca gtgcgcagat ctccgggctg agcgaggcga agggcatcgc gttggtggag
      301 gtttacgaaa tggacggcgt gtcacggctg ttgaacctgt cgactcgggc gcgtgtcgag
      361 agcggcgatg gaattctcat ttctggtctc gtgatcgcgg gcaaaggaac acgtcgcgtg
      421 ctgattcggg cgctcggacc ggtgctggcg gcggtgggcg tcgccggcac gctggccgat
      481 ccggtgttga gcgtgatcaa cgccggtggc gcggtggttg cgagcaacga caattgggaa
      541 gtggcgaacg gcagcacgaa catcgcggcg atcgcggcgg cggcgggcgc gggtgcgctc
      601 ggcgcgggca gcaaagattc ggcgttggtg gcggatctgc cggcgggcgt ttacacgacg
      661 catgtgcgcg gcggcgatgg cggcagcggc gtggcgttgc tggaagttta cgacgtttcg
      721 gcggtcggcg aactgtcgca gtcggcgccg gcgaccggcg gttgggagcc gggctacagc
      781 gaagcagcgc tgagctgggc gccggcggat gtatccacgc ggctgggacc gaccttcgaa
      841 gagttgaacc cgggcgcggc ggacgatcgg ccgacgctgt ttcagcgcga gtcgaagatg
      901 gcgggcagca aatattatct gcgcatcaat ccttacgagc tcggtgagcc gggcagttac
      961 aacgattact ggagcgatac gggccaggtg gcctacgttc ccgacgatgc ggcgaacgat
     1021 cccggtctcg atcgcgtgca actctacgcc tactacaaca aagttttcgc gatcagcccg
     1081 cgcctcgatt ggggcagcgg caagccgcat ccggacccgc aaacgcgcga gccgaactac
     1141 atcaagttga acggcgggac gatgccgatg cagcccgtcg cgatggcgcg cggctacggc
     1201 atgcagcaga acgagcaggt gatggtttat cgcgatggtt tgttcgcagt ggccggcatg
     1261 cagacgagcc gcaacggttc ggagcggccg tatccaggat tcaaattccc cgcgcacaag
     1321 gtgccgcgcg cgatcgcggt gacgacgagc aacgagttcg cgctggtgac gatttgggat
     1381 accgaacgca cgcagggtca gcttgcggtc gtggcgctcg aggcgaagtt cctgccgttt
     1441 cacacgtggc cctacatggg tttgccgaac cagggcagtt tctcggatgt gaagctgctg
     1501 ggttacatcg atctcccgat gaagtcgccg aatgcggtga gcgcggcgag caatgggctg
     1561 tggctcgggc cgtcttcgac ggcgggcaaa gtgctcagcc agatcgatct cgcggacgac
     1621 gcgagtcgcc agctcgttta tgatggcgcg tggcagtcgg tggtcgcgaa aagcggctat
     1681 gcgatcgtga gctccaccga agacaacaaa gcggtgatcg tcgatctcac gccgctgttt
     1741 tcctacatgc gcgagagcta tctcggttcg agcgagagct tccagcgcac gattgcgtcg
     1801 cgcggaccgg cgccggaaca gtttccgcag acgttcgcgg tcaatcccgc gagcatgccg
     1861 cgggtgatct
//
LOCUS       DASPLT010000908         1548 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_196039, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000908 DASPLT010000000
VERSION     DASPLT010000908.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1548)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1548)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1548
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_196039"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            154..681
                     /locus_tag="VEA63_09425"
     CDS             154..681
                     /locus_tag="VEA63_09425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD84263.1"
                     /translation="MQIVLGDDPSAGGVLLFGDLSYPTIRRIFDETKAAGQEENLAWQ
                     VMLAPHHCSKSVMYQDEDGTLTLKQDILDDIEAAMVGMGTIVASCEAIPASNIKGDNP
                     PHAKAKARYQEIAGAFFCTHEDGKSGEPLFFSVGDDATIDYVELGVPSDDPVAGAVDS
                     ARGGSAPPSDKVGFG"
     gene            678..1547
                     /locus_tag="VEA63_09430"
     CDS             678..1547
                     /locus_tag="VEA63_09430"
                     /inference="COORDINATES: protein motif:HMM:NF025813.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD84264.1"
                     /translation="MTWSAGQTLAREQLEDIQSAANGHVVIEQVIDPHDEASRAKIVV
                     SLTTAHLRRAPNGLPLKAREKLVLHIPREFPFESPSVHAAHKRWAGFPHVQWGDYLCL
                     FQSTNIEYTPSDGMFGFIDRLNIWLAAGALGELDPEDAPLHPPSVAATTDERVVIYAD
                     APTIQTDDWHWIGAARCTRRNERRLDLTEWIPLDKDFPVGGEKHTIAAAVILNKPLPF
                     EYPTTVRTLVVELVRHGLCLSLLMKVTQGDFDRAFLVYTAEDQPHLPGEIESYPLSRI
                     DELAASLARESAK"
BASE COUNT          327 a          498 c          452 g          271 t
ORIGIN      
        1 ggagcaaacg gctgctcaaa cgggcgcaac cgccgagccg aagcctgacc agtcagcgca
       61 aacaatctcc agcgagcacg acgcttccgc gccttcatcc atgcgccgtt caaggacgac
      121 agtgcgggcg agcgcaacga aacaagcgtc gcgatgcaga ttgtcctggg ggacgacccg
      181 tcggcgggcg gcgttctgct tttcggcgat ctgagctacc cgacgatccg cagaatcttc
      241 gacgaaacga aagcagcggg tcaggaggag aatctcgcct ggcaggtgat gctcgcaccg
      301 caccattgca gcaagtctgt gatgtaccaa gacgaggacg gcacgctcac gctgaagcaa
      361 gacatccttg acgacattga agcggcgatg gtcggcatgg gcacgattgt ggcgagctgc
      421 gaggcgatcc cggcaagcaa catcaagggt gacaatcctc ctcacgccaa ggccaaggcg
      481 cgctaccaag agatcgccgg agcgttcttc tgcacgcacg aggatggaaa gtcaggcgaa
      541 ccactcttct tctccgtagg cgacgacgca acgatcgact acgtcgaact cggcgttccg
      601 tcagacgatc cggttgcggg cgcggtcgac agcgcacgcg gagggtccgc gccgccgagc
      661 gacaaggtcg gttttgggtg acctggagcg ccggccaaac gctcgcccgc gaacaactcg
      721 aagacatcca gtccgctgca aacggtcatg ttgtcatcga gcaggtgatc gacccgcacg
      781 acgaagcgag tcgggcgaaa atcgtcgtga gcttgaccac cgctcacctt cggcgcgcgc
      841 ctaacggctt gccgctcaag gcgcgggaga agctcgtcct tcatatcccg cgtgagtttc
      901 cgtttgaaag ccccagcgtt cacgccgcgc acaaacgctg ggcgggtttc ccccacgtgc
      961 aatggggcga ctatctctgc ctgtttcagt ccaccaacat cgaatacacg ccttcggacg
     1021 gcatgttcgg gtttatcgat cggctcaaca tctggctcgc ggccggcgcg ctcggggaac
     1081 ttgatccaga ggacgcaccg ttgcatccgc cgtccgttgc cgcgacgaca gacgagcgtg
     1141 tcgtcatcta tgcggatgcc cctaccatcc aaaccgacga ctggcactgg atcggcgcgg
     1201 cccgttgcac tcgccgaaat gagcgtcgcc ttgatctcac tgagtggatt cccctcgata
     1261 aggacttccc tgtaggaggt gaaaaacaca cgattgcggc ggcggtgatc ctcaacaagc
     1321 cgctgccatt cgaatacccg accacagtga ggacgctcgt cgtcgagctt gtccgacatg
     1381 gcctttgcct cagtctgttg atgaaggtca cccaaggcga cttcgaccgc gccttcctcg
     1441 tctacaccgc cgaagaccag cctcaccttc ccggtgaaat agaatcctac cccctctccc
     1501 gtatcgacga gcttgccgct tctctcgcga gggagagcgc caaatgac
//
LOCUS       DASPLT010000909         2295 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_134449, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000909 DASPLT010000000
VERSION     DASPLT010000909.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 2295)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2295)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2295
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_134449"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..549)
                     /locus_tag="VEA63_09435"
     CDS             complement(<1..549)
                     /locus_tag="VEA63_09435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HYD84265.1"
                     /translation="MPRSPLPPLLFADTVTSADQLYFSRVDVHDPFIAFGTGTKRITV
                     QSALEFGRIKKASTFDEVLPLETWRERATQRHRGRPAGPAEIIAELARAYRVRGFRVA
                     DDFPAALFLKLRDLGVTLELANGPLFPEREIKSPAEAAAIREGNRLSSVGFTVAEKIL
                     RASRPRGRTLVYERQPLTSERLR"
     gene            599..1141
                     /locus_tag="VEA63_09440"
     CDS             599..1141
                     /locus_tag="VEA63_09440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009510994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HYD84266.1"
                     /translation="MKNANPVWASRMWRLGVLLFACALAGCSRPAPATSETAPKSVVD
                     FFEIRVGEKVVRMQLAVRPGEMERGLMERRDLGRDEGMLFVYEHPQQLSFWMRNTPTP
                     LDIGFFNRHGMLEEIYPMHPFDETPVRSRSTLLSFALEMNQGWFRENGVKPGAQLDVD
                     ALAAALKARGFAPERYGVGR"
     gene            complement(1261..1707)
                     /locus_tag="VEA63_09445"
     CDS             complement(1261..1707)
                     /locus_tag="VEA63_09445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD84267.1"
                     /translation="MAKIDVSKVAEILKKNEIDPAVLRRVMEEMNLVVQPEPGEDDKP
                     PAVKKQFAILVSDPENKLPPKTDLVGWVLQLPETESVATTQERIFRASYDYNATKKGR
                     LYPAKTVGEALENIPAKHFKEADVWVKTKTPVLVLKTDNEIPKAEA"
     gene            1874..>2295
                     /locus_tag="VEA63_09450"
     CDS             1874..>2295
                     /locus_tag="VEA63_09450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HYD84268.1"
                     /translation="MNESKPSTNTFTLELGASAPDFRLPATDGKTYAPGDFADAKVLV
                     VFFTCNHCPYVTGSDETTRRTAERFAAQGVRFVGINSNSVNTYAEDSFEHMIERMKQH
                     NFPWVYLRDESQDVARAYGALRTPHFYVFDEQRRLVYTG"
BASE COUNT          399 a          566 c          881 g          449 t
ORIGIN      
        1 gcggaggcgc tcggacgtga gcggctggcg ttcgtagacg agggtgcgac cgcgcggacg
       61 cgaggcgcgg agaattttct cggcgacagt gaagccgacg gagctgaggc ggttgccttc
      121 gcgaatcgcg gcggcttcgg cgggtgattt gatttcgcgt tcggggaaaa gcggaccgtt
      181 ggcgagctcg agcgtgacgc cgaggtcgcg aagcttgaga aaaagcgcgg cgggaaaatc
      241 gtcggcgacg cgaaaaccgc gcacgcgata cgcgcgggcg agctcggcga tgatttcggc
      301 cgggccggcg gggcggccgc ggtggcgttg agtcgcgcgt tcgcgccagg tttcaagggg
      361 caggacttcg tcgaacgtgc tggctttctt gatacggccg aattcgagcg cgctctggac
      421 cgtgatgcgt ttcgtgcctg tgccgaacgc gatgaaggga tcgtggacgt cgacgcggct
      481 gaagtagagt tggtcggcgc tggtgacggt atcggcgaaa agaaggggcg ggagcggaga
      541 gcgtggcacg atgagacgaa gattgagttg cgggtcggcg aggattagcc accgggcgat
      601 gaaaaacgca aatccggttt gggcgtcgcg gatgtggagg ctcggggtgt tgttgttcgc
      661 gtgcgcgctc gcgggctgca gccggccggc gccggcgacg agtgaaacgg cgccgaaaag
      721 cgtggtggat tttttcgaga ttcgcgttgg cgagaaggtc gtgcggatgc aactggcggt
      781 gaggcccggg gaaatggagc gcgggctgat ggagcggcgc gatctcggcc gcgatgaagg
      841 catgctcttc gtttacgaac acccgcagca gctgagtttt tggatgagga acacgccgac
      901 gccgctcgat atcggttttt tcaaccggca cgggatgctg gaggaaattt atccgatgca
      961 tccgtttgac gaaacgccgg tgcgttcgcg gagcacgctg ctgtcgttcg cactcgagat
     1021 gaaccaggga tggtttcgcg agaatggcgt gaagccgggc gcgcagctcg acgtggacgc
     1081 gctcgcggcg gcgctgaagg cgcgggggtt tgcgccggag aggtatgggg tggggcgtta
     1141 gcgggtgacg tcgacgacgg cgacggccgc ggggcgaacg cggagggaga ggtgcgcggt
     1201 caccggtggg aaggcgggag tggagtcggg gcgtgaagcc cctcccaccg tttttctgac
     1261 tcaggcttct gccttgggga tctcgttgtc ggttttcagg acgagcacgg gggttttcgt
     1321 ttttacccag acgtcggctt ccttgaaatg tttggcgggg atgttttcga gcgcttcgcc
     1381 gacggttttc gcgggataaa ggcggccttt tttggtggcg ttgtaatcgt aggacgcgcg
     1441 gaagatgcgc tcctgcgtgg tggcgacgct ctcggtctcg gggagctgga gcacccaacc
     1501 gacgagatcg gttttcgggg ggagcttgtt ttcgggatcg gagacgagga tcgcgaactg
     1561 cttcttcacc gcgggcggct tgtcgtcttc gccgggctcg ggctgcacga ccaggttcat
     1621 ttcctccatg acgcgacgga gcacggcggg atcgatttcg ttcttcttga ggatttcagc
     1681 gaccttgctg acgtcgattt tggccatggt gaaaaaaagg agcaaggagg catgctgcca
     1741 aggtcggcaa gtttcaaggc cggacaccgc aggggtgtag aaaggggctg agcgaagccg
     1801 tctcgggcgg aaagcgacgc gcgtgttgtc ggaagcgtct tgcgccgagg cggttcgttt
     1861 gcagggtcag cgcatgaacg agagcaagcc ttcgaccaat acgttcaccc tggagctggg
     1921 agcgagtgcg ccggactttc ggctgccggc gacggatggg aagacttacg cgccgggcga
     1981 ttttgcggac gcgaaggtgc tggtggtgtt cttcacgtgc aaccactgcc cttatgtgac
     2041 gggttcggac gaaacgacgc ggcggacggc ggagcgattt gccgcgcaag gcgtgcgctt
     2101 cgtcgggatc aattcgaaca gcgtgaacac gtatgcggag gattcgttcg agcacatgat
     2161 cgagcggatg aagcagcaca attttccgtg ggtttacctg cgcgacgaga gccaggacgt
     2221 ggcgcgggct tacggcgcgt tgaggacgcc gcacttttat gtcttcgatg agcagcggcg
     2281 gctggtttac acggg
//
LOCUS       DASPLT010000910         1281 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_154348, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000910 DASPLT010000000
VERSION     DASPLT010000910.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1281)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1281)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1281
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_154348"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..181)
                     /locus_tag="VEA63_09455"
     CDS             complement(<1..181)
                     /locus_tag="VEA63_09455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD84269.1"
                     /translation="MNPNHPDPLPCGSVPASCRSLHPKLLAAPFCRVLIGLALALSFL
                     SHRLPAGTRSMDFFGV"
     gene            complement(360..1064)
                     /locus_tag="VEA63_09460"
     CDS             complement(360..1064)
                     /locus_tag="VEA63_09460"
                     /inference="COORDINATES: protein motif:HMM:NF019242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1573 domain-containing protein"
                     /protein_id="HYD84270.1"
                     /translation="MSRPSEPWPAAFAFHGFTPRAWPLLGAFLFLSGPNLAHAALSWA
                     RTHLDAAIHASAGPTEFRYAFRNDGDTDVTLIDVQPSCGCTTGKSAKKTYRPGESGEL
                     VVIFTPDAPDRTQRSEIKVTTSDAPERPVVLTLETVTYEVFAVSPSVVRWYLHENASA
                     KSAFIALAPGFQADVSIVSFDREWLEVALVSDSSGYKVWAKPVSTAVPRRAEIRLRLT
                     RADGISHPILLRAQVR"
     gene            complement(1048..>1281)
                     /locus_tag="VEA63_09465"
     CDS             complement(1048..>1281)
                     /locus_tag="VEA63_09465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HYD84271.1"
                     /translation="LPKSKFAQYASGLDIIQSLAVMLIGPAMGAVLDWTHHSYRYTFL
                     ASFVVAIAAIVAAVVVYRRFMVLGGPQHYVAPE"
BASE COUNT          255 a          366 c          457 g          203 t
ORIGIN      
        1 tcacgccgaa gaagtccatc gaacgcgtgc ccgcgggcag tcggtggctg aggaacgaca
       61 gtgcaagcgc gaggccgatc aggacacggc agaatggggc ggcgaggagt ttggggtgaa
      121 gagaacggca cgaagccggg acgctaccgc aggggagcgg gtcggggtgg tttggattca
      181 tattggggag ggaacggtcc cattttttgc gcggcgcgtg attttgcaca aacgtgcgaa
      241 gacctgacac gtcatgttgc ggcgcacgtg ctgtcgcgaa gctggcggcg gtttcgcacg
      301 gcgacgttca tcacggccga gattgccgcc gcggtggaca ttcagcggcg gcattccgct
      361 cagcgaacct gcgcacggag caggatggga tgggaaatcc cgtcagcgcg cgtcagccga
      421 aggcggatct ccgcgcgtcg cggaaccgcc gtggagacgg gtttcgccca aactttatat
      481 ccagaagagt ccgacaccag agccacctcg agccattcgc ggtcgaagga gacaatcgac
      541 acatctgctt gaaagcccgg ggccagcgcg atgaacgcgc ttttggccga ggcgttttcg
      601 tgcaaatacc agcgcacgac cgagggagac accgcgaaca cctcgtaggt gacggtctcg
      661 agtgtgagca cgacaggacg ctccggggcg tcgctggtgg tcaccttgat ctcgctgcgc
      721 tgcgtgcgat caggcgcgtc aggggtgaaa atcaccacga gctcgcccga ttccccgggc
      781 cggtaggttt tcttcgccga tttgccggtg gtgcagccgc agctcggctg cacgtcgatg
      841 agcgttacgt ccgtgtcgcc gtcgttgcgg aaggcatagc ggaactccgt cgggccggcg
      901 gacgcgtgga tcgcggcgtc gagatgggtg cgggcccacg acagtgcggc gtgggcaagg
      961 ttcggtccgg ataaaaagag gaacgcgccc agcagcggcc acgcgcgcgg cgtgaagccg
     1021 tggaacgcga aagcagcggg ccaaggttca ctcgggcgcg acatagtgct gcgggccgcc
     1081 tagcaccatg aagcggcgat acaccacgac cgcggcgacg atcgcggcga tcgcaacgac
     1141 aaagctggcc aggaacgtgt agcggtagga gtgatgcgtc caatccagca ctgcgcccat
     1201 cgccggacca atgagcatga cggcgaggct ttggatgata tcgaggcccg acgcatattg
     1261 cgcgaacttg gatttcggca g
//
LOCUS       DASPLT010000911         1131 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_175354, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000911 DASPLT010000000
VERSION     DASPLT010000911.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1131)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1131)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1131
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_175354"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..455)
                     /gene="smpB"
                     /locus_tag="VEA63_09470"
     CDS             complement(<1..455)
                     /gene="smpB"
                     /locus_tag="VEA63_09470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007363659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HYD84272.1"
                     /translation="MCAQKKDASRYTEIRNSKALRDYFVDERFEAGIQLRGTEVKSIR
                     GGRAQINDAFGRVEKGELWLHGAHIEQYAFGNIYNHDAKRIRKLLLHKHQVRKIQQAL
                     ETGGRALVPLRMYFKDALVKVEVGLCTGKKLFDKRETLKKKVEMRDVEKA"
     gene            complement(537..737)
                     /locus_tag="VEA63_09475"
     CDS             complement(537..737)
                     /locus_tag="VEA63_09475"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HYD84273.1"
                     /translation="MRDDYVKEALKVIPDPNLLINVVSRRVKQLRRGNRPLVESLEKL
                     SSEDLALREIIEGKISFETGEN"
     gene            860..>1131
                     /gene="cutA"
                     /locus_tag="VEA63_09480"
     CDS             860..>1131
                     /gene="cutA"
                     /locus_tag="VEA63_09480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010038 - response to metal ion [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="divalent cation tolerance protein CutA"
                     /protein_id="HYD84274.1"
                     /translation="MSAPSSPPPLFIAWTTVAQRTDADTLAAGAIAAGLAACVQIDGP
                     IVSHYRWNGKAERAEEFRLTFKFLAEHAAALERHIHERHPYDTPEW"
BASE COUNT          194 a          403 c          290 g          244 t
ORIGIN      
        1 gccttctcga cgtcccgcat ctcgactttc ttcttcagcg tctcgcgctt gtcgaaaagc
       61 tttttgccgg tgcacaaccc cacctcgacc ttcaccagcg cgtctttgaa atacatccgc
      121 agcggcacga gcgcccgccc gcccgtttcg agcgcctgct gaatctttcg cacctggtgt
      181 ttgtgcagca gcagctttcg gatccgcttc gcgtcgtgat tataaatgtt gccgaacgcg
      241 tattgctcaa tgtgcgcgcc gtgcagccac aactcgccct tctccacgcg gccaaacgcg
      301 tcgttgatct gcgcccgtcc gccgcgaatc gacttcacct cggtgccgcg caactgaatg
      361 cccgcctcga agcgctcgtc cacgaagtag tcgcgcaacg ctttcgaatt gcggatctcc
      421 gtgtaacggc tggcgtcctt tttttgcgcg cacatgggtt gggtcggcta tatctatgca
      481 caaagaaggc ggctccggca atcgcggggc cgcctccgtg gaaaattcgc tgtcgctcag
      541 ttctcgccgg tttcgaaact gatcttgcct tcgatgatct cgcgcagcgc caaatcctcg
      601 gaagacagct tttcgagcga ctcgacgagc ggacggttgc cgcgccgcag ttgcttcacg
      661 cggcgtgaaa ccacgttgat caacaggttc ggatccggaa taaccttgag cgcttctttg
      721 acgtagtcgt ctctcatagt aggcgtttct ctgcggtgaa aaacgttgag agctacaccg
      781 gccgtccgaa gcgtcaaggg tcaatttccc cggcgccgcg tgcttccgtc cccgcgccca
      841 ccgccgccct ccattcgcca tgtccgcccc atcttccccg cctccgcttt tcatcgcttg
      901 gaccaccgtc gctcaacgca ccgacgccga caccctcgcc gccggtgcga tcgccgccgg
      961 cctcgccgcc tgcgtgcaaa tcgatggccc catcgtctcg cactaccgct ggaacggaaa
     1021 agccgaacgc gccgaggagt ttcgcctcac cttcaagttc ctcgccgagc acgccgctgc
     1081 gctcgaacgc cacatccacg aacgccatcc ttacgacacg cccgaatgga t
//
LOCUS       DASPLT010000912         1217 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_34508, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000912 DASPLT010000000
VERSION     DASPLT010000912.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1217)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1217)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1217
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_34508"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..520
                     /locus_tag="VEA63_09485"
     CDS             <1..520
                     /locus_tag="VEA63_09485"
                     /inference="COORDINATES: protein motif:HMM:NF012234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HYD84275.1"
                     /translation="DGGTSTRLVGQFLYWLQEARARVFVVATANDVSRLPPELLRRGR
                     FDELFFVDLPTPEERAEIVNIYAKRGLKQAVNGGLLEELVTLADGFAGADIEAAVREV
                     TKGAFLDGDGTVTPDLFRRSFMNIVPLSKTNPEAVEAIRVWGRERAVPASGQPIGGSQ
                     AASPRARRSVLV"
     gene            532..1128
                     /locus_tag="VEA63_09490"
     CDS             532..1128
                     /locus_tag="VEA63_09490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD84276.1"
                     /translation="MARPLIGQVTQPSAPPAAVAAAGSSSGPTEPPRMPAGVRWYWFT
                     AALLAIALTSTAAWWYVSRATPIRLWTPLVAGEEHAIGGQLYLDEDAAGKIVGRSTTA
                     EGVAYLELEIGVSPSRRAKLKHGLVRLTGQRALWLTSEFIEEPAPPLVNGDVVPAVDP
                     EQRYAMRWWKKLDSWLLTTGGVGVAWLFIKGCLRFRRR"
BASE COUNT          214 a          331 c          433 g          239 t
ORIGIN      
        1 ggacggcggc acgtcgacgc gactggtcgg ccagtttctt tactggctgc aggaggcgcg
       61 ggcgcgtgtc tttgttgtcg cgaccgcgaa cgatgtttct cgactgccgc cggaacttct
      121 ccgacggggc cgcttcgacg aattgttttt tgtggatcta ccgacaccgg aggaacgggc
      181 ggaaatcgtg aacatttatg cgaagcgtgg cctgaagcag gcggtcaatg gaggtcttct
      241 cgaggagctg gtgacgctcg cggatggatt tgcgggagcg gacatcgagg cggcggtgcg
      301 tgaggtgacg aagggggctt tcctcgatgg agatggaacg gtgacgccgg atctcttccg
      361 acggagcttc atgaacatcg tcccgttgtc gaaaacgaac cctgaggccg tcgaagcgat
      421 tcgggtctgg ggccgcgagc gcgcggtgcc ggcgtctggc cagccgatcg gcgggtccca
      481 agcggcctct ccgcgcgcgc gccgatccgt cctcgtttag tcacgagcta tatggcgcgt
      541 cccctcatcg gccaagtgac acagccatcc gctccgccgg cggcggtggc ggcggcgggt
      601 tcttcctccg gaccgaccga gccgccacgc atgcctgcgg gcgtccgctg gtattggttc
      661 acggccgcgc tgctagcgat cgcgcttacg tcaacggctg cctggtggta tgtgagtcgt
      721 gcgacgccga ttcggttgtg gactccgttg gtcgcaggag aagagcacgc gatcggaggc
      781 cagctttatc tggatgaaga tgcggcaggg aaaatcgtcg gccgaagcac gacggcggaa
      841 ggcgtggcat atcttgaact cgaaatcggc gtgagtccgt cacgtcgggc aaagttgaag
      901 catggtctcg tgcgtctcac cggccaacgt gcgttgtggc tgacaagtga gttcatcgag
      961 gaaccggcgc cgcccttggt gaatggcgat gtggtgccgg cggtggaccc cgaacaacgg
     1021 tatgcaatgc ggtggtggaa aaaactggat tcgtggcttc tgacgaccgg cggggtcggc
     1081 gtggcatggc ttttcatcaa gggctgcctg cgtttccgca ggcgttgata gcgcgagcgt
     1141 gatcggagct tcgaatgaac taccactatc aagacgcgac tcagaaggcg gtgggaccga
     1201 tgagcgaaga tgagctg
//
LOCUS       DASPLT010000913         1002 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_134559, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000913 DASPLT010000000
VERSION     DASPLT010000913.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1002)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1002)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1002
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_134559"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..512)
                     /locus_tag="VEA63_09495"
     CDS             complement(<1..512)
                     /locus_tag="VEA63_09495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD84277.1"
                     /translation="MRSSTSSPSSGYADLRTQIVASGIIEAADITDITPLSGGVSSDI
                     VLVRCAGHAPFVVKRALGQLRVRDPWFADVSRNRIEQAWLAYAARLAPASVPRMLHRA
                     DTWFAMEYLGTELTPWKTELLAGRVDLRHAQRAGEFLGAIHRASWADPEAARVFATGR
                     GFHELRIEPYL"
     gene            571..>1002
                     /locus_tag="VEA63_09500"
     CDS             571..>1002
                     /locus_tag="VEA63_09500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HYD84278.1"
                     /translation="MAGIDIFLAALLEALLCDVKIHFEKVLPNAGESFHCRRFEEPTF
                     PAPYHTHPEIELTYIVSSHGRRFVGDSIEQFGPGDLVLIGSNLPHRWASDTPPPKAGR
                     RWATSLVVQFLPDCLGAHFWNQPEFAAIATLLARAQAGLVFP"
BASE COUNT          184 a          309 c          311 g          198 t
ORIGIN      
        1 agatagggct cgattcgcaa ttcgtggaag ccacgacctg tggcgaagac tctggctgcc
       61 tccggatcgg cccatgaggc ccggtggatc gcgccaagga attcaccggc gcgttgcgca
      121 tgcctcaggt cgacgcggcc cgcgagcaac tctgttttcc acggagtgag ttccgtgccc
      181 agatactcca tagcgaacca cgtgtccgca cgatgcaaca tccgcggcac ggacgcgggt
      241 gcgagccgcg cggcgtatgc gagccacgcc tgctcgatgc ggttgcggct gacatccgcg
      301 aaccacggat cgcggacgcg gagctgacca agggcgcgtt tgacgacgaa cggcgcatgg
      361 cccgcgcatc gcacgagcac gatatcgctg gaaactccgc ccgacagcgg ggtgatatcc
      421 gtgatgtcgg ctgcctcgat gatcccgctc gccacgatct gagttcgaag atcggcatag
      481 cctgatgacg gcgacgaggt ggaggagcgc atacgcgggt tggaagggcg ccaacgttac
      541 gagattcagc cgcggaaaac ttcctcgcgc ttggctggaa tcgacatttt tttagccgcc
      601 ttgctcgaag cgctcctttg cgatgtgaag attcattttg aaaaagtcct gcccaacgcg
      661 ggcgaatcct ttcactgccg gcgctttgaa gagcccacct tccctgcgcc ttaccacacg
      721 catcccgaga tcgagctgac ctatattgtt tcgagtcacg gccgacgttt cgtaggcgat
      781 tcgatcgagc agtttggccc aggtgacctg gtgttgatcg gttcgaatct tccccatcga
      841 tgggcaagcg acacgccgcc gccgaaagcc ggtcgccggt gggcgacctc gctcgtggtg
      901 cagttcctgc cggactgtct cggcgcgcac ttctggaatc agccggagtt tgcagcgatc
      961 gcgacattgc tggcgcgtgc ccaagctgga cttgttttcc cg
//
LOCUS       DASPLT010000914         3430 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_134560, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000914 DASPLT010000000
VERSION     DASPLT010000914.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 3430)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3430)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3430
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_134560"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..501
                     /locus_tag="VEA63_09505"
     CDS             <1..501
                     /locus_tag="VEA63_09505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD84279.1"
                     /translation="YIETHRDLCTETPEKLAEIAARYRRATGELLPVMWDHSHLAVMK
                     HVLPEDFSRRLLADRALIQRSRMFHCRPFNSQHCQVPVTNGRGRLTPEFRDYLVFVED
                     LFTVWLEGPKPRGDLWVCPEMGTTVGYHVSTNPPVWPDTLRCREELLAAWARARVRTQ
                     KKRARR"
     gene            572..1615
                     /locus_tag="VEA63_09510"
     CDS             572..1615
                     /locus_tag="VEA63_09510"
                     /inference="COORDINATES: protein motif:HMM:NF024770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HYD84280.1"
                     /translation="MLLREIANKARVSPATVSRAINQPQLVAPDSLARIRAVMLQNNY
                     EPAPLNRRRGPKTRLPEQRRIGVWFVGARANNPSLNWFQDQLLQVQSTDPHYRVDLRV
                     LFSNTPNDLPRNLAAEQLDGVIVQGMEPSAACLRELRELPHVWFMTRRSAFYPGDYVE
                     PNNAENGRMAADYLKERGHQSVAVISTDPDYSAIVSRNHAFVERARELGLAVNTILGR
                     SNPGVSYLEIAPLHGESDELVRHLLESTPKATGLYIPVDHFCGAFFRALREAGKKPGQ
                     DFEAILGNYNPVIYHNLDHSPAAIDINLPTLVRKVIDHLLWRIENPGVTGRVGVTVSP
                     RLLTPDSPNRAIK"
     gene            1872..>3430
                     /locus_tag="VEA63_09515"
     CDS             1872..>3430
                     /locus_tag="VEA63_09515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HYD84281.1"
                     /translation="MVLSPFVVTGQDDRGYQATSTLGGTRLRTDLRNIGSSISIVNEA
                     FLQDTGSANLEDVLIFTPNTEVGGLGGNYSASQGSGNPVPEQQRDNQSGGITRVRGLA
                     SADLTRDYFITNIPFDTFNTDRVEVQRGANSALFGLGSPGGIVNSSTIKADFRSNHGR
                     IRAETDSYGTQRYSLRYNHVLNNYVALHVAALNEDTRYEQRQAFNKDRRYFAAVTVKL
                     PFNIVARGSYETSKREASRPDYIPPNDGITPWINLGKPVFDSPAQAGAFFRGTGGFIP
                     GVANSAFLTPVSGGTGAGMISFYGDPNNPNPTFGGASHIRGGRGIPSAGGLGEWMMLQ
                     PFTETQIIRRSGYRSDGTAIPAGTGGFYSNGFVGTQITDRSIFDYRKNLFSGGSSQEF
                     ADWVVYTAAVEGTWLDGRLGVELSYYNQAMNTSGYNALQGSEQRTIYIDPNRYLINTT
                     DGTGTGTLVANPSFGQPVMGGLSGGNMIVDDREAKRATGFFELRARDFMERNWVSKLL
                     GRLTVTGLVEG"
BASE COUNT          663 a         1208 c          927 g          632 t
ORIGIN      
        1 tatatcgaaa cgcatcgcga cctctgcacc gaaacgccgg agaaactcgc cgagattgcg
       61 gcgcgctacc gtcgagcgac cggcgaactc ctgcccgtga tgtgggatca ttcgcacctc
      121 gccgtaatga agcacgtcct gccggaggat ttttcgcggc ggctcctcgc cgaccgcgcg
      181 ttgattcaac gctcgcgcat gttccactgc cgtccgttca acagccagca ttgccaggtg
      241 ccggtcacga atggacgcgg ccggttgacg cccgaatttc gcgactacct cgtcttcgtc
      301 gaagaccttt tcaccgtctg gctggaaggc ccgaagccgc gcggcgattt gtgggtgtgc
      361 cccgaaatgg gcacgaccgt gggctatcac gtgagcacca atcctccggt ctggccggac
      421 acgctgcgct gccgtgaaga gttgctcgca gcgtgggcgc gcgctcgcgt tcgcacacaa
      481 aagaagcgcg cccgccgctg aaaacgcttg accgggacgg gcgtcatttt catgaggatt
      541 gctgaaattt tcagaacgca taccccaccc catgctgctt cgcgaaatcg ctaataaggc
      601 ccgcgtctcg cccgcgacgg tttcgcgcgc gatcaatcag ccgcaactcg tcgcccctga
      661 cagcctcgcg cgcatccgcg cggtgatgct gcagaacaac tacgagcccg cgccgctgaa
      721 tcgccggcgc ggccccaaga cccgcttgcc cgaacaacgg cgcatcggcg tgtggttcgt
      781 gggcgcccgc gcgaacaacc ccagcttgaa ctggtttcaa gaccagctgc tgcaggtgca
      841 atccacggat ccgcactatc gcgtcgattt gcgcgtgctt ttttccaaca cgcccaacga
      901 cctcccgcgc aacctcgcgg ccgagcaact cgacggcgtc atcgtgcaag gcatggagcc
      961 gtcggccgcc tgcctgcgcg aactgcgcga gttgccgcac gtctggttca tgacccgtcg
     1021 ttcggcgttc taccccggcg actacgtcga accgaataac gccgagaacg gccgcatggc
     1081 cgccgactac ctgaaggagc gcggccacca gtccgtcgcg gtgatttcca ccgatcccga
     1141 ctacagcgcg atcgtcagcc gcaaccacgc tttcgtcgaa cgtgcgcgcg agctcggcct
     1201 cgcggtgaac accattctcg gccgttccaa tcccggcgtc agctatctcg agatcgcgcc
     1261 gctgcacggc gagagcgacg aactcgtgcg ccatctcctg gaaagcacgc cgaaggcgac
     1321 cggcttatat attcccgtcg accatttctg cggcgcgttt ttccgcgctc tgcgcgaagc
     1381 gggcaagaaa ccgggtcagg atttcgaagc catcctgggc aactacaacc ccgtcattta
     1441 ccacaacctc gatcactccc ccgcggcgat cgacatcaat ctccccacgc tggtccgcaa
     1501 ggtcatcgac cacctgctct ggcgcatcga gaaccccggc gtcacgggtc gggtcggcgt
     1561 caccgtctcc ccgcgtctgc ttacgcccga ctctcccaat cgtgcgatca agtaacctcg
     1621 ctttcgaaaa acgagacagc cgcgtcgctg tctcccgttc ctttgtccgc ctcctttgag
     1681 ccggataatt ccaattcgcg gctaaacccc cgaaatcccc aaacccaaaa gagcaccgaa
     1741 gaatgaaaac acacaccaaa ccgtggctac tcgccccact attgacgata tgtgcgggag
     1801 gaatgttcgc ccagagcgcc ccctcaaccc cggcaaccgg ctcgacgtcc gaccctgcga
     1861 atcaggatcc cgtggtcctg tctcctttcg ttgtcaccgg ccaggatgac cgcggctatc
     1921 aagcgacgtc cacccttggc ggaacccgtc tgcgcaccga tctccgtaac atcggttcct
     1981 cgatttccat cgtcaacgaa gcgttcctcc aggacaccgg ctccgccaat ctcgaggacg
     2041 tgttgatctt cacacccaac accgaagtcg gcggtctggg cggcaactat tccgcctccc
     2101 aaggttccgg caacccggtt cccgagcagc agcgcgataa ccaaagcggc ggcatcacgc
     2161 gtgttcgcgg attagcctcc gccgatctga cccgtgatta tttcatcacg aatattccgt
     2221 tcgacacgtt caacaccgat cgcgtcgagg tgcaacgcgg cgcgaattcc gccctcttcg
     2281 gtctcggcag cccgggcggt attgtgaata gctccacgat caaggccgac ttccgctcca
     2341 accacggtcg catccgcgcc gagacggata gctacggcac gcagcgctac tcgcttcgtt
     2401 acaatcatgt tctcaacaac tatgtggcac ttcacgtcgc cgcgttgaac gaggataccc
     2461 gctatgaaca acggcaggcc ttcaacaagg accgccgcta tttcgccgcg gtcaccgtaa
     2521 agctgccctt caatatcgtc gcccgcggca gctacgaaac gtcgaaacgc gaggcatcgc
     2581 gccccgacta tattccgccg aatgacggca tcacgccctg gatcaatctc ggcaaacccg
     2641 tgttcgattc gcccgcgcaa gccggcgcat tcttccgcgg caccggcggt ttcatccccg
     2701 gcgtcgccaa cagcgcgttc ctcacccccg tctcgggcgg cacgggcgcg ggcatgatct
     2761 cgttctacgg cgatccgaac aacccgaacc cgaccttcgg cggcgcctcg cacatccgcg
     2821 gaggtcgcgg cattccctcc gccggcggtc tcggcgaatg gatgatgctg cagccgttca
     2881 cggaaaccca aatcattcgt cgctccggct atcgctcgga cggcacggcg attcccgctg
     2941 ggaccggcgg cttctacagc aacggcttcg tcggcacgca gatcaccgac cgcagcattt
     3001 tcgattaccg caagaacctg ttctccggcg gttcatccca ggagttcgcc gattgggtcg
     3061 tttacaccgc agccgttgaa ggcacctggc tcgacggccg cctcggtgtg gaactctcct
     3121 actataatca ggcgatgaac acctccggtt acaacgccct tcaaggcagc gaacagcgca
     3181 caatctacat cgatccgaac cgctacctga tcaacacgac cgacggcacc ggcacaggaa
     3241 ctctcgtggc caacccgtcc ttcggtcagc cggtgatggg cggtctttca ggcggcaaca
     3301 tgatcgtcga cgatcgcgaa gcgaagcgcg ccaccggctt cttcgaactc cgcgcccgcg
     3361 actttatgga acgcaactgg gtctcgaagt tgctcggccg gttgaccgtg accggtttgg
     3421 ttgaaggcag
//
LOCUS       DASPLT010000915         1016 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_196134, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000915 DASPLT010000000
VERSION     DASPLT010000915.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1016)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1016)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1016
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_196134"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            66..>1016
                     /locus_tag="VEA63_09520"
     CDS             66..>1016
                     /locus_tag="VEA63_09520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside monophosphate kinase"
                     /protein_id="HYD84282.1"
                     /translation="MPTSIPAEARPASPTPIAPVAGKLTDLEIKDAQLIFGSVWQNLE
                     ADFGRENLRFPKEIILLGGAPGAGKGTNTAFIAKTRGLTCPPIVISALLDSPEARRLK
                     DAGNMVGDREVVSLLLRELMNPIYRDGVILDGFPRTNVQVECLKLLVDKMHALRREFF
                     NTPLNIHFRQPTIHIMVLFIDERESIARQLKRGRETLIHNEEVRRTGIGELWEDRATD
                     HDEALAQRRYRVFKEHTWDALQSLKEIFHYHFINAQGPIEEVEQNILKELEYQSTLEL
                     DPRTVDRLRGVPVASEIIVHSRQELVKRLDSYEFGQPELFA"
BASE COUNT          209 a          361 c          264 g          182 t
ORIGIN      
        1 ctcatttctt ttgtcgaatc tgtcacggaa acccgctaac tcctcgcgct tctcgctcga
       61 acctcatgcc cacctccatc cccgccgaag cgcgtcccgc ctccccgact cccattgccc
      121 ccgtcgccgg caagctcacc gacttggaaa tcaaggacgc tcaactgatc ttcggttccg
      181 tctggcagaa tctcgaagcc gacttcggtc gcgagaatct ccgcttcccc aaggagatca
      241 tcctgctcgg cggcgcgccc ggcgccggca agggaacgaa caccgctttc atcgcgaaaa
      301 cccgcggcct cacctgcccg ccgatcgtca tcagcgcgct gctcgattcg ccggaagccc
      361 gacgcttgaa agacgcgggc aacatggtcg gcgaccgcga ggtcgtgagc ttgttgctcc
      421 gcgagttgat gaaccccatc taccgcgacg gcgttatcct cgacggcttc ccccgcacga
      481 acgtgcaggt cgagtgttta aagctgctcg tggacaaaat gcacgcgttg cgccgcgagt
      541 ttttcaacac gccgctcaac atccattttc gccagcccac gattcacatc atggtgctct
      601 tcatcgacga gcgcgaatcg atcgcccgcc agctcaaacg cggccgcgaa acgctcatcc
      661 acaacgaaga ggtgcgccgc acgggcatcg gcgaactctg ggaagatcgc gccaccgacc
      721 acgacgaagc gctcgcgcag cgccgctacc gcgtcttcaa ggaacacacg tgggacgcgc
      781 ttcagtcgtt gaaggaaatt ttccactacc acttcatcaa cgcgcagggg ccgatcgaag
      841 aggtcgagca gaacatcctc aaggaactcg aataccagag caccctcgaa ctcgatcccc
      901 gcaccgtcga ccggttgcgc ggcgtgccgg tcgccagcga gatcatcgtt cattcgcgcc
      961 aggaactcgt gaagcgcctc gacagctacg aattcggcca acccgagttg ttcgct
//
LOCUS       DASPLT010000916         3882 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_71252, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000916 DASPLT010000000
VERSION     DASPLT010000916.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 3882)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3882)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3882
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_71252"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..628)
                     /locus_tag="VEA63_09525"
     CDS             complement(<1..628)
                     /locus_tag="VEA63_09525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HYD84283.1"
                     /translation="MSGLFSSLNSAVSALTAHSRSVETAGKNLANVSNPSYARQRVIY
                     GDRGTVVTPDGARSLGLEAIGVQQLRDKLLDRQVVREGALAAYYQAQQSAYQRAQAGL
                     GQDVASATNALSSTSSASDTGIAAALDDLFNSFQNFASNPTDIGQRQSLLQNASILTD
                     RIGLADTRLAQVQEDLNSQIENDVTDANILLSTIADLNGQIGRFEINQP"
     gene            complement(675..1166)
                     /locus_tag="VEA63_09530"
     CDS             complement(675..1166)
                     /locus_tag="VEA63_09530"
                     /inference="COORDINATES: protein motif:HMM:NF016985.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlgN"
                     /protein_id="HYD84284.1"
                     /translation="MNWQSIADSLRQELAEYGALLHLFEQQQESLFKRDPDNVLRLGT
                     EIEDQAHILQDCRGRREQAVAGFATLLGLPASSTIRSLLPHVEADARPLLEALINEIN
                     VLLHRVRRTSRHNHTLLSRTLELHQETLRQLRPDAFTKTYSPSGRVSLAATAGAPSLR
                     AAG"
     gene            complement(1163..1552)
                     /locus_tag="VEA63_09535"
     CDS             complement(1163..1552)
                     /locus_tag="VEA63_09535"
                     /inference="COORDINATES: protein motif:HMM:NF021638.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod-binding protein"
                     /protein_id="HYD84285.1"
                     /translation="MNVVPLNATSPSAADALADLAAPAKLRAKPLTGAALRNATPELQ
                     RKEVAAQFEAILVRQLLKPTMASMFSAESGAAGGIYGDMLTDTFATQLTRGGGLGLGR
                     MLEQQLTPRSPEAALNASATLATPSST"
     gene            complement(1561..2700)
                     /locus_tag="VEA63_09540"
     CDS             complement(1561..2700)
                     /locus_tag="VEA63_09540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HYD84286.1"
                     /translation="MTQPGHILSFLRFGHLGRLLAAFVVFHSSFVMLHASRVKDLTLV
                     EGGRDNQIVGYGIVVGLAGDGDSNAATTLRSVSNILARYGLTVNSTDIKAKNAAAVMI
                     TADIGAFLKPGARIDVNVASMGDAKSLQGGVLLQTPLLGADGRVYAVAQGAVAIGGFL
                     GGTGGAGGATVQKNHPTVGVISSGGIVEREIPSTFVRDNQLRLLLHNPDFTSAARMAS
                     AINARWPGASAAIDAATVSVALPADYAGQDVAFIADLGTVEVQPDTLARIVINERTGT
                     IVATSTVRLSQVAIALGSLTITVSSNVGVSQPNGFSQGQTVAVPSTQTDVAETKGGFT
                     VLNEPPTIERLAAALNALGVSTREMMAILQGLKRSGALQAELVIN"
     gene            complement(2759..3412)
                     /locus_tag="VEA63_09545"
     CDS             complement(2759..3412)
                     /locus_tag="VEA63_09545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009427 - bacterial-type flagellum basal
                     body, distal rod, L ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HYD84287.1"
                     /translation="MKTVSKSASNRRSVFAFAVVLAVTAGGAPLAAANGSLWPANAGS
                     ERGMIADRKASRAGDILTIVISETAIAQSSQSKKSSRDSNIDDAVQQFFFANSRALTH
                     GGGLPGLNLGGSSSYSGGGDVSNSQSLSARAAVLVSDVLPNGNLVIQGARVVSFSGET
                     QYVVLHGIVRGDDVLRDNTVASSNIADARVEFYSEGQLTEAQKRGWFSKLYEKLRPF"
     gene            complement(3384..>3882)
                     /gene="flgA"
                     /locus_tag="VEA63_09550"
     CDS             complement(3384..>3882)
                     /gene="flgA"
                     /locus_tag="VEA63_09550"
                     /inference="COORDINATES: protein motif:HMM:TIGR03170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="flagellar basal body P-ring formation chaperone
                     FlgA"
                     /protein_id="HYD84288.1"
                     /translation="QLEMLDYPSVASSSMLVRCRVLADAEAVAEWTLVLRATLWRDAW
                     VSRQPLVTGETFDPSVLEVRRVDVLRERSALPVEVGDRSFAIARSVTAGRLLTWHDIQ
                     RRPLVRKGNLVEVSAADGQLLITMKALAMENGAQGETVTVRNPESRKDFAAMVVDENR
                     VQVRF"
BASE COUNT          672 a         1068 c         1463 g          679 t
ORIGIN      
        1 cgggctggtt gatctcaaag cggccaatct ggccgttgag gtcggcgatg gtgctgagaa
       61 ggatgttggc gtcggtgacg tcgttctcga tttgcgagtt aagatcctcc tgcacttgcg
      121 cgagccgcgt atcggcgagt ccgatacgat cggtgagaat gctggcgttc tggagcaggc
      181 tttggcgctg accgatgtcg gtcgggttgc tcgcgaaatt ttgaaacgaa ttgaaaagat
      241 cgtcgagggc cgcggcgatg ccggtgtcgc tcgcgctgct ggtggagctg agtgcgttgg
      301 tggcgctggc gacgtcctga ccgaggccgg cctgcgcgcg ctgatacgcg ctttgttgcg
      361 cttgatagta ggcggcgagc gcgccttcgc gcacgacctg gcgatcgaga agcttatcgc
      421 ggagctgctg cacgccgatc gcttcgaggc cgaggctgcg ggcgccatcg ggcgtgacga
      481 ccgtgccgcg gtcgccgtag atgacgcgct ggcgggcgta gctcgggttg ctgacattcg
      541 ccagattctt acccgcggtc tcgaccgagc ggctgtgcgc ggtcagcgcg ctgacggcgg
      601 agttgagact ggagaaaagg ccggacatga cgaaacggaa gctgagagct gagagtctgg
      661 agctgagagc ccgatcagcc ggcggcgcgg agagagggcg cgccggcggt ggcggcgagc
      721 gagacgcggc cgctgggcga gtaggttttc gtgaacgcgt cggggcgaag ttgacgaagc
      781 gtttcctggt gcaattcgag cgtgcggctg agcagcgtgt gattgtggcg gctggtgcgg
      841 cggacgcggt ggagaagaac gttgatttcg ttgatcaacg cttcaagcag cggacgcgca
      901 tcggcctcga cgtgcggcag cagcgagcga atcgtggagg aggcgggcaa gccgagcagc
      961 gtggcgaaac cggcgacggc ttgttcgcgg cggccgcggc aatcctgaag gatgtgcgcc
     1021 tgatcttcga tttcggtgcc gagacgaagc acgttgtcgg ggtcgcgttt aaaaagactt
     1081 tcctgttgct gctcgaaaag atggaggagc gcgccgtatt cggcgagttc ctggcggaga
     1141 gagtcggcga tcgattgcca gttcatgtgg aagaaggagt cgcgagggtg gcggacgcgt
     1201 tgagcgccgc ctcggggctg cgcggagtga gttgttgctc gagcatgcgg ccgaggccga
     1261 ggcctccgcc gcgcgtgagt tgggtcgcaa aagtgtcggt gagcatgtcg ccgtagatgc
     1321 cgccggcggc gccgctctcg gcgctgaaca tcgaggccat cgtgggcttg agaagctggc
     1381 gaacgaggat ggcttcgaac tgcgcggcga cttccttgcg ctggagctcg ggcgtggcgt
     1441 tgcgcagagc ggcgccggtg agcggcttcg cgcgcagttt cgcaggcgcc gcgaggtcgg
     1501 cgagtgcgtc ggcggcggag ggagaggtgg cgttgagcgg gacgacgttc atcgcagcga
     1561 tcagttgatc acgagctcgg cctggagcgc gccggagcgt ttgagaccct gaagaatggc
     1621 catcatctcg cgcgtggaaa cgccgagggc gttgagcgcg gccgcgagac gttcgatcgt
     1681 tggcggctcg ttgaggacgg tgaagccgcc tttggtttcc gcgacgtcgg tctgcgtgct
     1741 cgggaccgcg acggtctggc cctggctgaa cccgttcggc tggctgacgc cgacgttgga
     1801 actgacggtg atcgtgagcg agccgagcgc gatggcgact tgcgagaggc gaacggtcga
     1861 cgtcgcgaca atggtgccgg tgcgctcgtt gatcacgatg cgagcgagcg tatcaggttg
     1921 gacctcgacg gttccaagat cggcgatgaa cgcgacgtcc tggccggcgt aatcggcggg
     1981 caacgcaacg ctgaccgtcg ccgcgtcaat ggcggcggac gcgccgggcc agcgggcgtt
     2041 gatggcggag gccatgcgcg cggcggaggt gaaatcggga ttgtgcagga gaaggcggag
     2101 ctggttatcg cggacgaacg tcgacgggat ttcacgttcg acgataccgc cgctgctgat
     2161 gacacccacg gtcggatgat tcttttgcac ggtggcaccc cctgccccgc ccgtgccgcc
     2221 gaggaaaccg ccgatcgcca cagcgccctg cgcgaccgcg tagacgcggc cgtcggcacc
     2281 gagcaacggc gtctggagca gcacgccgcc ctgcagcgat ttcgcatcgc ccatcgaggc
     2341 gacgttcacg tcgatgcgcg cgccgggttt taggaacgcg ccgatgtcgg cggtgatcat
     2401 cacggcggcg gcgttcttgg ccttgatgtc ggtggagttc accgtgaggc cgtagcgtgc
     2461 cagaatattg gatacgctgc gaagagtcgt cgcggcgttg ctgtcgccgt cgccggcgag
     2521 gccgacgacg atgccgtagc cgacgatttg attatcgcgc ccgccttcga cgagcgtgag
     2581 gtccttcacg cgggaggcgt gaagcatgac gaacgacgaa tggaaaacga cgaacgccgc
     2641 cagcaagcgg ccgaggtgac cgaagcggag gaaactgaga atgtgtccgg gctgagtcat
     2701 tcggaggtca attcgtcgtt cgtgaagttg gagattcgtc atatgcgcgt gccgcgcatc
     2761 agaacggacg gagcttttcg tagagtttgg agaaccaacc gcgcttctgc gcctcggtga
     2821 gctggccttc gctgtagaac tcgacgcggg cgtcggcgat gttggacgaa gcaacggtgt
     2881 tgtcccggag cacgtcgtcg ccgcggacaa taccatgaag gacgacgtat tgggtttcgc
     2941 cggagaagga gacgacgcgc gcgccctgga tgacgagatt gccattgggc aggacgtcgc
     3001 tcacgagcac ggccgcgcgg gcggaaaggc tttgcgaatt ggagacgtcg ccgccgccgg
     3061 agtagctcga ggaaccgccg agattcaagc cgggcaaacc gccgccgtgc gtgagcgcgc
     3121 ggctgttcgc gaagaagaac tgctggacgg cgtcgtcgat gttcgagtcg cgcgaggact
     3181 ttttgctctg cgaactctgc gcgatggcgg tttcgctgat gacgatggta agaatgtcgc
     3241 ccgcgcgaga ggctttgcga tcggcgatca tgccgcgttc gctgccggca ttggccggcc
     3301 agagcgatcc gttggctgcc gcgagcggcg cgccaccggc ggtgacggcg agcacgacgg
     3361 caaacgcgaa gacgctgcga cggttagaag cggacttgga cacggttttc atcgacgacc
     3421 atcgcggcaa aatccttccg cgattccggg ttgcgaacgg tgacggtttc gccctgcgcg
     3481 ccattttcca tggcgagggc tttcatagtg atgagcaatt ggccgtcggc ggcggagacc
     3541 tcgacgaggt tgcctttgcg gacgagcggg cggcgttgaa tatcgtgcca ggtgagaagc
     3601 cggccggcgg tgacggagcg ggcgatcgcg aacgaacggt cgccgacttc gacgggcagc
     3661 gcggagcgct cgcggagcac gtcgacgcga cgaacttcaa ggacggaagg atcgaaggtt
     3721 tcgccggtga cgagcggttg gcgtgaaacc caggcgtctc gccaaagggt ggcgcgcagg
     3781 accagcgtcc attcggcgac ggcttcggcg tcggcgagca cgcggcagcg cacgagcatg
     3841 gaggaagacg cgaccgacgg atagtcgagc atctcgagct gc
//
LOCUS       DASPLT010000917         1796 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_92326, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000917 DASPLT010000000
VERSION     DASPLT010000917.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1796)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1796)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1796
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_92326"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1796
                     /locus_tag="VEA63_09555"
     CDS             <1..>1796
                     /locus_tag="VEA63_09555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HYD84289.1"
                     /translation="ARLTPAATTTAVCGFCSTGCGLKVHLDANGEAINLTADPAYPVN
                     LGKACPKGWEALTPLAANDRALTPLLRNGAGKLEAVSWSDAMTAFVTRFRRIQERHGA
                     AAVSFLSTGQIVTEEMALLGALAKFGMGMRHVDSNTRQCMATSHVAYKQSFGFDAPPF
                     TYADFEESDALIFIGANPCIAHPIMWQRVMMNRRNPEIVVIDPRRTETAIASTLHVAL
                     RPKSDLVLLYGLANLLIARGAVRWEFVAAHTNGFAEFETFVAEFTLERTAAETGVPAE
                     TLERLVTIIAERERVSFWWTMGVNQSHEATRTAQAIINLALMTGNIGRPGTGANSITG
                     QCNAMGSRLFGNATSLIGGYDFGKAEHRTHVAGILGIDAAVIPDAPGLAYDQILDAID
                     RGEIRGLWVIATNTAHSWINQRHFAALRAKLDFLVVQDMYPTTETAQMADLVLPAAGW
                     GEKDGVLINSERRLGLAKKVSRAPGQALSDFAIFKLIAEAWGCGEMFRAWRSPEAVFQ
                     ILKKLSRGQPCDFSGVRDYAHVDAEGGIQWPWTEADEQKKSEAGTPEAAEAKSERRHR
                     RLFADGKFFTADGRARFFFDPPRAMPEPPDEQ"
BASE COUNT          318 a          451 c          695 g          332 t
ORIGIN      
        1 ccgcgcgatt gacgccggcg gcgacgacga cagcggtgtg cggtttttgc agcacgggct
       61 gcgggttgaa ggtgcacctc gatgccaatg gcgaggcgat caatctcacc gcggatccag
      121 cgtatcccgt caatctaggc aaggcgtgcc ccaaaggttg ggaggcgctg acgccgctcg
      181 ccgcgaacga tcgtgcgctg acgccgttgt tgcgcaacgg cgcaggcaag ctggaagcag
      241 tgtcatggtc ggacgcgatg acggcgttcg tgacgcgttt ccgcagaatc caggagcgcc
      301 acggcgcggc ggcggtttcg tttttgagca ccggccaaat cgtgactgag gaaatggcgc
      361 tgctcggcgc gctcgcgaaa ttcgggatgg ggatgcgtca tgtggattcg aatacgcggc
      421 agtgcatggc gacctcgcac gtggcgtata agcaatcgtt cggtttcgac gcgccgccgt
      481 tcacgtatgc ggatttcgag gagagcgacg cgttgatctt catcggggcg aacccctgca
      541 tcgcgcatcc gatcatgtgg cagcgggtga tgatgaaccg gcggaatccg gagatcgtcg
      601 tcattgatcc gcggcgcacg gagacggcga tcgcgtcgac gctgcatgtg gcgttgcggc
      661 cgaagagcga cctggtgctg ctgtatgggc tggcgaattt gttgatcgcg cgcggcgcgg
      721 tgcggtggga gttcgtcgcc gcgcacacga acgggttcgc ggagttcgag acgtttgtgg
      781 cggagttcac gctcgagcgg acggccgcgg agacgggcgt gccggcggag acgctggagc
      841 ggctggtgac gatcatcgcg gagcgcgaac gggtgtcgtt ctggtggacg atgggcgtga
      901 accagagtca cgaagcgacg cggaccgcgc aggcgatcat caatctcgcg ttgatgacgg
      961 gaaacatcgg gcggccggga acaggtgcga actcgatcac gggccagtgc aatgcgatgg
     1021 gttcgcggtt gttcgggaat gcgacgtcgt tgatcggtgg atacgacttt ggaaaggcgg
     1081 agcatcgaac gcacgtggcg ggaattttgg gcatcgatgc ggcggtgatt cccgatgcgc
     1141 cggggttggc gtatgatcag attctggatg cgatcgatcg cggcgagatt cgcgggttgt
     1201 gggtgatcgc gacgaacacg gcgcattctt ggatcaatca gcggcacttc gcggcgttac
     1261 gcgcgaagct ggattttctc gtggtgcagg acatgtatcc gacgacggag acggcgcaga
     1321 tggcggatct ggtgttgccg gcggcgggct ggggcgagaa ggacggcgtg ttgatcaaca
     1381 gcgaacggcg gctggggctg gcgaaaaagg tgtcgcgcgc gccggggcag gcgttgagcg
     1441 atttcgcgat tttcaagttg atcgcggagg cgtggggttg cggggaaatg tttcgcgcgt
     1501 ggcgttcgcc ggaggcggtg tttcagattt tgaaaaaact gtcgcgcggg cagccgtgcg
     1561 atttcagcgg cgtgcgcgat tatgcgcatg tcgacgcgga aggcgggatt cagtggccgt
     1621 ggacggaggc ggatgagcaa aagaagtcgg aggcgggcac gcctgaagcg gcggaagcaa
     1681 agagcgagcg gcggcatcgg cggctgtttg cggatgggaa atttttcacg gcggacggac
     1741 gggcgcggtt tttcttcgat ccgccgcggg cgatgccgga gccgccggac gagcag
//
LOCUS       DASPLT010000918         2076 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_235885, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000918 DASPLT010000000
VERSION     DASPLT010000918.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 2076)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2076)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2076
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_235885"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..464
                     /locus_tag="VEA63_09560"
     CDS             <1..464
                     /locus_tag="VEA63_09560"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HYD84290.1"
                     /translation="GRRRLNLDRTRVRGVSFSADWKIAPSLTVNGQYLYDDARVARSA
                     VAPHLEGMRLAQVPRHAGTVGLTWRLGRWSLVPRVRWFGAQFEDDQNTLRLAAATIVD
                     MSASIALSKNAELFVSAENLLDERVETGRAATGLVNIGTPRLLLVGARLMR"
     gene            complement(648..1514)
                     /locus_tag="VEA63_09565"
     CDS             complement(648..1514)
                     /locus_tag="VEA63_09565"
                     /inference="COORDINATES: protein motif:HMM:NF016925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HYD84291.1"
                     /translation="MFYHDRKLQYHVRVEKPNPHFAKMLQQAIGGIEGEMRVCLQYLF
                     QAWGSRGPTKYRDLLLETGTEEIGHIEMLATAVALNLEGAPTETKSNAALHPVVNAVL
                     GGMDPRHVLSTGLAAMATDSNGVPFNGSWVVASGNLAADMYANVMAESTGRVLATRLY
                     EMTDDPGMKDMLSFLIARDTMHQNQWLAVLEDLGGLEKIHPIPNSFPQKMENQEFNYA
                     FVSTQMDAATDPMQRWTTGQSIDGKGTFSFQPAAPLGGEPVLAPPIPEGHAQKEEMAG
                     AMSDKVDRPLTR"
     gene            1843..>2076
                     /locus_tag="VEA63_09570"
     CDS             1843..>2076
                     /locus_tag="VEA63_09570"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HYD84292.1"
                     /translation="MRPPSNFDVAPGVFGSRSVMVNYYFVASTATPSSWVLVDAGLRG
                     SGLRLLREATRRFGANNPPRAIILTHGHFDHIGA"
BASE COUNT          334 a          661 c          661 g          420 t
ORIGIN      
        1 aaggccgccg tcgcttgaat ctcgatcgca cccgcgtccg cggcgtgtcg ttctccgcgg
       61 actggaagat cgcgccgagc ctcacggtga acgggcagta tctttacgac gatgcacgtg
      121 tcgcgcgctc tgcggtcgct ccgcatctgg aagggatgcg actcgcgcaa gtcccgcgac
      181 acgccggcac ggttggtctc acttggcgct taggccgctg gtcgctcgtg ccgcgcgtgc
      241 ggtggttcgg cgcgcaattc gaggacgatc agaacacgct ccgtctcgcg gcggcgacga
      301 ttgtcgacat gtccgccagc atcgccttga gcaagaacgc cgagcttttc gtgagcgccg
      361 aaaatcttct cgacgaacgc gtcgaaaccg gccgcgccgc cacaggcctc gtcaacatcg
      421 gcacgccgcg cctgttgctg gtcggcgcgc gattgatgcg gtgaggttcg ccgaacgtca
      481 cacagcttcg attcacgccg cgatggccgc cgtgatcgcc tgcgtttggt agcgcggtca
      541 atctgaccgc ggattgcgac gtcccgacac ggacaggttg accgtgctac gttgagccac
      601 gcctgaagcg atctccgttg cttcaggcgt ggccgggttt gcctggttca gcgcgtgagc
      661 gggcggtcaa ccttgtcgct catcgcgccg gccatttcct ccttctgcgc gtggccctcc
      721 gggatcggcg gagccagcac cggctcccca ccgagcgggg ccgccggttg gaaggaaaat
      781 gtccccttgc cgtcgatcga ctggccggtc gtccatcgct gcatggggtc ggtcgctgca
      841 tccatctgcg tcgaaacgaa ggcgtagttg aattcctgat tctccatctt ctgcggaaac
      901 gaattcggaa tcgggtgaat tttctccaac ccgccgagat cctccaaaac ggcgagccat
      961 tgattctgat gcatcgtgtc gcgcgcgatg aggaacgaca gcatgtcctt catgcctgga
     1021 tcgtcggtca tctcgtagag tcgcgtcgcg agcacgcgcc ccgtcgattc cgccatgacg
     1081 ttcgcataca tgtcggcggc gagattgccg ctggcgacga cccacgagcc gttgaacggc
     1141 acgccgttcg aatcggtcgc catggctgcg agaccggtgg acaggacatg gcgcggatcc
     1201 atcccgccga gcaccgcgtt gacgacagga tgcagcgcgg cgttcgactt tgtctcggtc
     1261 ggcgcgcctt cgaggttcaa cgccactgcg gtggcgagca tctcaatgtg gccgatttcc
     1321 tcggtgcccg tttccagcag caggtcgcga tacttcgtcg ggccgcgcga gccccacgct
     1381 tggaaaagat attggagaca cacgcgcatc tcgccttcga tgccgccgat cgcctgctga
     1441 agcattttgg cgaagtgcgg attcggtttt tcgacgcgga catggtattg aagttttcgg
     1501 tcgtggtaga acatggaacg aggaagtttt cttgaaggtt aagaagatcg ccgacggctt
     1561 ccgtctgccg gccggcgaag gggaaaccga ctctaccgcc gctacgacgc ccgcggtagt
     1621 agtgcggcgc tgcatccgca ggtggggtcc tcgcgcctcg ccggcatcgg gcatttcggg
     1681 gcgtgtcgcg gagtccgttg gacgcgagtc aaagcagtcc ttccgcagcc agcgccgggg
     1741 acgcgccgga tggctggcgc gctcggttga ggtagattcc gggacggcgc gtgaacggcc
     1801 cgccgctttg gagttcaact tcccgcgaat cctttctttc acatgcgacc gccctccaat
     1861 ttcgatgtcg cgccaggcgt attcggctcg cgcagcgtga tggtgaatta ctacttcgtc
     1921 gcatcgaccg ccacaccttc gtcgtgggtg ctcgtcgacg ccggtttacg cggctcgggg
     1981 cttcgcctgt tgcgcgaggc gacgcgtcgt ttcggagcga acaatccccc gcgcgcgatc
     2041 attcttacgc acggccactt cgatcacatc ggcgcg
//
LOCUS       DASPLT010000919         1034 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_134620, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000919 DASPLT010000000
VERSION     DASPLT010000919.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1034)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1034)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1034
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_134620"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(76..270)
                     /locus_tag="VEA63_09575"
     CDS             complement(76..270)
                     /locus_tag="VEA63_09575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD84293.1"
                     /translation="MPFPVPYHDTQPENAPAAIVRVLNREDIQWNRPLFRAPPLNVAP
                     PIKDVADLVRSVRKPDAILG"
     gene            complement(489..887)
                     /locus_tag="VEA63_09580"
     CDS             complement(489..887)
                     /locus_tag="VEA63_09580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HYD84294.1"
                     /translation="MHTIIQPSPARRHRRPASSSSTPMFRQPSYDCREHPDAVKLTVY
                     VPGVEASGVEIEARAPDLVVTARKPHFVRVNWQALHLETAQRDYQLRLRLGNGVDYPA
                     LHAEISDGVLTVVLPKRHAAEDNLLDRRVA"
BASE COUNT          218 a          260 c          383 g          173 t
ORIGIN      
        1 agagaggcga tcggtgccga caaaaaaaac ggctccgaaa atcggagccg cttgaaagag
       61 gaacagggca acgcgttaac caagaatcgc gtcgggcttg cgcacggagc ggacgaggtc
      121 ggcgacgtcc ttgatcggag gagcgacgtt gagcggtggg gctctgaaaa ggggacgatt
      181 ccactgaatg tcttcgcgat tcaaaacccg cacaatcgcc gccggagcat tttcaggctg
      241 cgtatcgtga tatggaacgg ggaatggcat aggggtggtg acctaagttt cggaacaagg
      301 tgccaacaac cttagggaaa tcttacgtgt caccgtaagg tgacgaaacg aacagggaaa
      361 agctgatggc gtttatcggt aaaatgaaaa ttcacgcgcg aaaccgggcg cttggaagcg
      421 cgcggcgtcg cgcaagaacg agagcgagaa cggagcgcgg gcggtgagcc gacgcgctcc
      481 gcttcgcgtt atgcgacgcg gcggtcgagg aggttgtcct cggcggcgtg gcgcttcggc
      541 aacacgacgg tgagcacgcc gtcgctgatt tcggcgtgca gtgccgggta gtcgacgccg
      601 ttgcccaagc gcaggcgcag ctggtagtcg cgctgcgcgg tttccaggtg caacgcctgc
      661 cagttcacgc ggacgaaatg cggcttgcgc gcggtgacga cgaggtcggg cgcgcgggcc
      721 tcgatttcca cgcccgacgc ttccacgccc gggacgtaga ccgtgagttt cacggcgtcc
      781 gggtgctcgc ggcagtcgta gctcggctgg cggaacatcg gcgtggacga cgacgaggcc
      841 gggcggcggt gacgacgggc ggggctgggt tggatgatcg tatgcatggg aaaagcggga
      901 gaacggaaat ggttggaaaa agggaaaagc aggatgctgc tcggggcttg tggccgccgg
      961 gcttcaggtg gtgttctaaa ctgggtccgt taaacggagg gtttaggcct gagagcccgg
     1021 gccggtctga tgct
//
LOCUS       DASPLT010000920         2290 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_235936, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000920 DASPLT010000000
VERSION     DASPLT010000920.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 2290)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2290)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2290
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_235936"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1192
                     /locus_tag="VEA63_09585"
     CDS             <1..1192
                     /locus_tag="VEA63_09585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HYD84295.1"
                     /translation="LSFFLKEKKGIISRCRDDYATKMRLRYAPYYHAMESQQGTSVKL
                     DGRDMIMLSSNDYLGLSFHPKVIEAGRAALLKWGTSTTGARPSNGSRTYHLQLEEKLA
                     AFLGREACHIHAAGYLSCLSSVASFAQKGDLIFADKNIHSCLWDGIRLSMATVERFSH
                     NSPDDLRSVLAATNSDAPKMLVAEGVYSMEGHIARLPELIDIADEHSCFSVIDDAHGF
                     GVLGRQGRGTVDHFGLNDRVDVICGSMSKSLASTGGFIAASREVIDYLRSHSRQTIFS
                     AAISASQAACAEASLEVMQTEPQHHERLWANTKKYRAMLKGLGLDIWGSETPAVPIVL
                     GSKERVYPFWRALMDKGIFTVMSIAPAVPAGKDLIRTAVSAMHTDEQLEKIAEAMAYA
                     VKRL"
     gene            complement(1222..>2290)
                     /locus_tag="VEA63_09590"
     CDS             complement(1222..>2290)
                     /locus_tag="VEA63_09590"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HYD84296.1"
                     /translation="STDDRRMIFFDVTKTQNVRHRSGLARMSERLRTELGAAASAGSW
                     TEIRESAGAHDWFVTSELFAEYERPGIGEFVRSRRCRTAAIFADAIPIKHPTITWPHS
                     VARHPHYMKALAGFERVWAISEASRDELHGYWEWLGVSNVPQIDVLPLGADFDGSPRV
                     TKREGATKGGVPALLCVGILEPRKNQVFLLEACAELWRAGLKFELHLVGRVNPHFGKP
                     VVARIREMKKEFRGLQYHEAADDATLAGLYARVRATVVPTIAEGCGLPLLESLWRGVP
                     CVCSDLPVLRENADGGGCVTVTLNDRAAWVQTLRDVLTDEARHARLVNEATTRVLPTW
                     AEAARVLREGLEDDDAVRTAP"
BASE COUNT          437 a          865 c          587 g          401 t
ORIGIN      
        1 gttatcattt ttcctgaaag agaaaaaggg catcatctcg cgttgccgcg acgactatgc
       61 gacgaagatg cgtctgcgtt acgcgcccta ctaccacgcc atggagtcgc agcagggcac
      121 cagcgtgaag ctcgacggcc gcgacatgat catgctctcg agcaacgatt atctcgggct
      181 ctcgtttcac ccgaaggtca tcgaagccgg tcgcgccgcg ctcctgaaat ggggcaccag
      241 caccaccggc gcccgcccgt cgaacggctc gcgcacttat catcttcaac tcgaggaaaa
      301 gctcgccgcc ttcctcggcc gcgaagcctg ccacatccac gccgcgggct atctctcctg
      361 cctctcgagc gtcgcgtcgt tcgcgcagaa gggcgatctc atcttcgccg acaaaaacat
      421 tcactcgtgt ttgtgggacg gcatccgcct ctcgatggcc acggtcgagc gtttctcgca
      481 caacagcccc gacgacctcc gcagcgtgct cgctgccacg aattccgatg cgccaaagat
      541 gctcgtcgcc gagggcgttt actcgatgga aggccacatc gcgcgacttc ccgagctgat
      601 cgacatcgcc gacgaacaca gttgcttctc cgtcatcgac gacgcgcacg gcttcggcgt
      661 tctcggccgt cagggccgcg gcaccgtcga tcacttcggc ctcaacgatc gcgtcgacgt
      721 gatctgcggc agcatgtcga aatcgctcgc gagcaccggc ggtttcatcg ccgcgtcacg
      781 cgaagtcatc gattaccttc gctcgcattc gcgtcagacg attttcagcg ccgccatcag
      841 cgcctcccaa gccgcctgtg ccgaagcctc actcgaggtc atgcaaacgg aaccgcagca
      901 tcacgagcgg ctctgggcca acacgaaaaa atatcgcgcg atgctcaagg gcctcggcct
      961 cgacatctgg ggcagcgaaa cgcccgccgt tcccatcgtc ctcggctcga aggagcgcgt
     1021 ctatccgttc tggcgcgcgc tcatggacaa aggcatcttc accgtcatgt cgatcgcgcc
     1081 cgcagtcccg gccggcaagg atctcatccg caccgccgtc tccgcgatgc acaccgacga
     1141 gcagcttgag aaaatcgccg aagcgatggc ctacgccgtc aaacggcttt gatctccaac
     1201 ggcgactcgt agccgcgccc gttagggtgc ggttctcacc gcgtcgtcgt cttccaaccc
     1261 ctcgcgcaac actcgcgccg cctccgccca agtcggcagc acgcgtgtcg tcgcctcgtt
     1321 caccaaccgc gcgtgacgcg cctcatccgt caacacgtcg cgcaacgtct gcacccacgc
     1381 cgcgcgatcg ttgagcgtca ccgtcacaca accgccacca tcggcgttct cgcgcaacac
     1441 cggcagatcg ctgcacacgc acggcacgcc gcgccacaac gattccagca acggcagccc
     1501 gcaaccttcc gcaatcgtcg gcaccaccgt cgcccgcacg cgcgcataaa gccccgcgag
     1561 cgtcgcgtcg tccgccgcct cgtgatactg caacccgcga aactcttttt tcatttcccg
     1621 aatccgcgcg accaccggct tgccaaaatg cggattcacc cgcccgacga ggtgcaactc
     1681 gaacttcaac cccgcgcgcc acaactccgc acacgcctcc aacaggaaaa cctgattctt
     1741 ccgcggttcc aaaatcccca cgcacaacaa cgccggcacg cctcctttcg tcgcaccctc
     1801 gcgcttcgtg accctcggcg atccatcgaa atccgcgccg agcggcagca cgtcgatctg
     1861 cggcacattc gaaaccccaa gccactccca atacccgtgc agctcgtcgc gactcgcttc
     1921 cgaaatcgcc cacactcgtt caaatcccgc caacgctttc atgtagtgcg gatgacgcgc
     1981 cacgctgtgc ggccacgtga tcgtcggatg ttttatcggg atcgcatccg caaaaatcgc
     2041 cgccgtccgg caccgccgcg aacgcacgaa ctcgccgatc cccggccgtt cgtattcggc
     2101 aaaaagctcg ctcgtcacga accagtcgtg cgcgcccgcg ctctcgcgga tctccgtcca
     2161 gctccccgcg ctcgccgccg ccccgagctc cgtgcgcagc cgctcgctca ttcgcgccag
     2221 cccggaacga tgacgcacgt tctgcgtttt cgtgacgtcg aaaaaaatca ttcgccgatc
     2281 gtccgttgaa
//
LOCUS       DASPLT010000921         1475 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_112617, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000921 DASPLT010000000
VERSION     DASPLT010000921.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1475)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1475)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1475
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_112617"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..281
                     /locus_tag="VEA63_09595"
     CDS             <1..281
                     /locus_tag="VEA63_09595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD84297.1"
                     /translation="WKVLLIVVLALVAIKLLPVLLVPVLVVGLAAMIATGLLLGGFGV
                     IAAAGLSVLAGLLAVVLVVLAVLSPIWIPVLVVVGIVALVRRGTRTHA"
     gene            419..1114
                     /locus_tag="VEA63_09600"
     CDS             419..1114
                     /locus_tag="VEA63_09600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD84298.1"
                     /translation="MTDELPSSVVREIAFVGRSGGQVKALGGFRKGHHTLPDAANDTT
                     NAFLGKICAGELGEEAEALFQAVRAGLGYKRKDVTLALASPHAVLAAKDFSVEIDYQL
                     EAAEPTRYAVTTTLRELRSAELARSEAMSAVFARRFSEISFALNKGASVEAVVDAIEG
                     LDVEKGLSVSYPSDCSECEIRVEGVDAHVRCTATALEMVFPRAGAPRELIDAFAQVRA
                     AFRIDRELAGLIG"
BASE COUNT          226 a          354 c          574 g          321 t
ORIGIN      
        1 tttggaaggt tttgctgatc gtcgtgctcg cgctggtggc gatcaaattg ctgccggtgt
       61 tgctggtgcc ggtgttggtc gtcgggctgg cggcgatgat cgccacgggg ctgctgttgg
      121 gcggttttgg ggtgatcgcg gcggcgggtt tgagcgtgct cgccgggttg ctggcggtgg
      181 tgctggtcgt gctcgcggtg ctgtcgccga tctggattcc ggtgctcgtg gtggttggga
      241 ttgtggcgct ggtgcggcgc gggacgcgga cgcacgcgtg aagcggcatg ctttggcgct
      301 cgcttccgtt tcggttgagc tttgcgcggc gggggcgccg cggctccacg tgcgtggagg
      361 gaacgatggc tgattttcgg gttcgcgtgg aggcgcggcg ttcgtttgct cgcggtggat
      421 gaccgatgaa cttccctcca gcgtggtgcg cgaaatcgcg ttcgtggggc gcagcggcgg
      481 gcaggtgaag gcgctgggcg gatttcgaaa agggcatcac acgttgccgg acgcggcgaa
      541 cgatacgacg aacgcgtttc tcggaaagat ctgtgccggc gagttggggg aggaagccga
      601 ggcattgttt caagcggtgc gagcggggct tggttacaag cggaaggatg tgacgctcgc
      661 gctcgcgagt cctcacgcgg tgctggcggc gaaggatttt tcggtggaga tcgactacca
      721 gctggaggcg gcggagccga cgcgttacgc ggtgacgacg acgttgcggg aactgcgcag
      781 cgcggagttg gcgcggagcg aggcgatgtc agcggtgttc gcgcggcgct tttcggaaat
      841 ttcttttgcg ttgaacaaag gagcctcggt cgaggcggtg gtggatgcga tcgaggggct
      901 ggacgtggag aaagggctgt cggtgagtta cccgtcggat tgcagcgagt gcgagattcg
      961 ggtggagggc gtcgatgcgc acgtgcgttg cacggcgacg gcgttggaga tggtgtttcc
     1021 gcgggcgggc gcgccgcggg agttgatcga cgcgttcgcc caggtgcgtg cggcgttccg
     1081 gatcgatcgg gagttggcgg ggttgattgg gtgaggggga tcgaagaagc tgaatggcga
     1141 agtatggctg acaccaaggc ggaagcggag cggtagttca ctcatatata gtctgctacg
     1201 catttgatgt tccaaaatgg aacatcaaat ggttctctcc aaatttgcgg agcggagcat
     1261 ccacattcgc gttgagcacg cacgtgccgg cgctttgcct tgattccaaa ttcgcggagc
     1321 ggagcatcct cggcccagac ctgcaggtcg aactcttgta agcgtccgac cgaagacctc
     1381 gtgcgtcggt attgctggtg cccgatattc cggccagcgg gtgaccatcg ttacgtctcg
     1441 aaggtgacct tcaagatgaa cccgcattcc gttac
//
LOCUS       DASPLT010000922         1535 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_11588, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000922 DASPLT010000000
VERSION     DASPLT010000922.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1535)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1535)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1535
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_11588"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1215
                     /locus_tag="VEA63_09605"
     CDS             <1..1215
                     /locus_tag="VEA63_09605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase C-terminal beta-propeller
                     domain-containing protein"
                     /protein_id="HYD84299.1"
                     /translation="LTGLERGKIEAEYLELMKTIEELRGILESEPKLLALIKKELGEM
                     KEKYTSPRRTEIIGTAGEFRMEDVIPNEGCVITVSHLGFIKRTPVAEYRSQKRGGKGI
                     IGAETYDEDFVENLFTASTHDYILFLTSTGQCHAKKVYDVPEGTRTSKGKSVSSFLRL
                     TDGEKIAAMLCVKDFVNDQFLVMATKKGTIKKSSLSDYANATREGGLIGINLSEGDSV
                     IGTVLTTGADELILVSHQGLAVRFRERDPETNEELFRATGRATGGVTGMRFKLASDYL
                     QVIEVVDHQAKFLVASEAGLGVRTRFEDYRLINRGGSGVWAIDLPEDGSIKLAGALSV
                     KDTDEIMLLTAKGQSVRCPVNTIRETNRGAKGVRLVNLQPGDKLLSIARIVETEEEAG
                     SAETPPPATTTP"
BASE COUNT          333 a          538 c          411 g          253 t
ORIGIN      
        1 ctcaccggcc tcgagcgcgg caagatcgaa gccgaatacc tcgagttgat gaaaaccatc
       61 gaggaactcc gtggcatcct cgagtccgag ccgaagctgc tcgcgttgat caaaaaagag
      121 ctcggcgaga tgaaggaaaa atacacctcg ccgcgtcgca ccgagatcat cggcaccgca
      181 ggcgagttcc gcatggaaga cgtcatcccc aacgagggct gcgtcatcac cgtttcccac
      241 ctcggcttca tcaagcgcac acccgtcgcc gaataccgct cccagaaacg cggcggcaag
      301 ggcatcatcg gcgcggagac ctacgacgag gatttcgtcg agaacctctt caccgcctcg
      361 acgcacgact acattctctt cctcacgagc accggccagt gtcacgcgaa gaaggtctac
      421 gacgtccccg aaggcacccg cacttcgaag ggcaaatccg tctcgtcctt cctccgcctc
      481 accgacggcg agaaaatcgc cgcgatgctg tgcgtgaagg atttcgtcaa cgaccagttc
      541 ctcgtcatgg cgacgaagaa gggcacgatc aaaaagagct ctctctccga ctacgccaac
      601 gccacgcgcg aaggcgggct catcggtatc aacctgtccg aaggcgacag cgtgatcggc
      661 accgtgctca cgaccggtgc cgacgagctc atcctcgttt cgcatcaagg cctcgccgtc
      721 cgcttccgcg aacgcgatcc cgagaccaac gaagaactct tccgcgccac cggtcgcgcg
      781 acgggcggcg tcacgggcat gcggttcaaa ctcgcgagcg attatctgca ggtcatcgag
      841 gtggtcgatc atcaggcgaa attccttgtc gcgagcgaag ccggcctcgg cgtccgcacg
      901 cgcttcgaag attaccgcct gatcaaccgc ggcggcagcg gcgtgtgggc gatcgacctg
      961 cccgaagacg gctcgatcaa actcgccggc gcgttgagtg tgaaggacac cgacgaaatc
     1021 atgctgctca cggcgaaagg ccagagcgtg cgttgtccgg tgaacacgat tcgcgaaacc
     1081 aaccgcggcg cgaaaggcgt gcgtctggta aacctccaac ccggcgacaa actcctgagc
     1141 atcgcgcgca tcgtcgagac cgaggaagaa gccggcagcg ccgaaacgcc accgcctgcg
     1201 accacaacgc cctgatcgcg tcaaagcgca cctcgctgtg ggaggggctt tacgccccga
     1261 cctccccgcg cttccggtga ggacataaaa cctttctcac caaaggcccg ctctccgagc
     1321 gggccttttc atttatacac caaaacgcgt gtgcccaccg ggcgccggtt ttgccactta
     1381 agaaacctca cgcgatcaac ctctctctac aacctgcgcc gtttccgcct tctccaacgc
     1441 cctcaaaatc cgccgcaccg tccgcgcctc ggcctttctc gtcagttcgt gctcccaaat
     1501 ccgcagcacg cgccaccctg ccttcttcaa cagcc
//
LOCUS       DASPLT010000923         1202 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_175502, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000923 DASPLT010000000
VERSION     DASPLT010000923.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1202)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1202)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1202
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_175502"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..778)
                     /locus_tag="VEA63_09610"
     CDS             complement(<1..778)
                     /locus_tag="VEA63_09610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HYD84300.1"
                     /translation="MKSLLLVFLLVVFVLGSNAALTAAPTARVYLTQKDSAHRLSPIG
                     SLEFTPLPQPPEHQQCVFIDPSKKFETLLGIGGAITDASAETFAKLTADKQAELLQAY
                     YDAEHGIGYSLARTTIHSTDFSSASYTYVEDGDRELESFDIAPDLKHRIPLLRAALKT
                     AGEDLTIYASPWSPPAWMKDSGSMLHGGKLLPEFREPWANYFVKFIEAYEKAGVPIWG
                     VTVQNEPMAVQTWESCVFTAEEERDFIRDHLGPTLHAHGLA"
     gene            complement(871..>1202)
                     /locus_tag="VEA63_09615"
     CDS             complement(871..>1202)
                     /locus_tag="VEA63_09615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD84301.1"
                     /translation="ENHGTLWVNYKFTGNALEGLSIGAGGWWEDERSIYPAYGQNALD
                     SDGNPIFLSAKERTSISAMVKYEFMFRGRPSSVQLNVDNVLNDRDLYGFIYAAPRRWQ
                     ITFTHRL"
BASE COUNT          234 a          375 c          359 g          234 t
ORIGIN      
        1 acgccagacc gtgcgcatgg agcgtcggcc cgaggtgatc gcggatgaaa tcgcgttcct
       61 cctccgccgt gaacacgcag gattcccagg tctgcaccgc catcggctcg ttctgcaccg
      121 tcacgcccca gatcggcacg ccggccttct cgtaagcctc gatgaacttc acgaaatagt
      181 tcgcccacgg ctcgcgaaat tctggcagca gtttcccgcc gtgcagcatg ctgccgctgt
      241 ccttcatcca cgcgggcgga ctccatggac tcgcataaat cgtgagatcc tcacccgccg
      301 tcttcagcgc ggcgcggagc agcggaatcc ggtgcttcag atcgggcgcg atgtcgaaac
      361 tctccaactc gcggtcgccg tcctccacat aggtgtagct ggcggaggaa aaatcggtgc
      421 tgtgaatcgt ggtgcgcgcg agcgaatagc cgatgccgtg ctccgcatca tagtaggcct
      481 gcaacaattc cgcctgcttg tccgccgtca gtttcgcaaa cgtctccgct gacgcgtccg
      541 tgatcgcacc gccgatgccg agaagtgttt cgaatttctt cgaaggatcg atgaagacgc
      601 attgctggtg ctcgggcggc tgcggaagcg gcgtaaattc gagcgagcca atgggcgaga
      661 gccggtgcgc agaatctttc tgcgtaagat aaacacgggc cgtcggggca gcggtgagcg
      721 cagcgtttga tcccaagaca aacacgacta gcaaaaagac gaggagcaat gacttcatgg
      781 tatagcgacg gtcgagcggc ccgcccggag gtcgggccct acccctcagc cctcttcgtt
      841 cattcgtggt tccacttggc caaacgtcca tcacagccgg tgcgtgaacg tgatttgcca
      901 gcgacgcggc gcggcgtaga tgaagccgta gaggtcgcgg tcgttgagca cgttgtcgac
      961 gttaagctgc acgctcgagg ggcgaccgcg gaacatgaac tcgtatttca ccatcgcgct
     1021 gatcgaggtg cgctccttcg ccgagaggaa aatcggattg ccgtcgctat cgagcgcgtt
     1081 ctggccgtaa gcgggataga tcgagcgctc gtcctcccac caaccgcccg caccgatgct
     1141 cagtccctcg agcgcattgc cggtgaactt gtagttcacc cacaacgtgc cgtgattctc
     1201 cg
//
LOCUS       DASPLT010000924         1133 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_215057, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000924 DASPLT010000000
VERSION     DASPLT010000924.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1133)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1133)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1133
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_215057"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            312..1055
                     /locus_tag="VEA63_09620"
     CDS             312..1055
                     /locus_tag="VEA63_09620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD84302.1"
                     /translation="MKTKFLRTCLLAPTLAAFAATPALLASEASARAEAAISEVKGDA
                     AKAALTELDAEMDRLETLIDSAPTPEDKAAARARLDALKERRAELRKTYVKARYDELK
                     ADVRAEANRVGNWAKRTFNRDEASNAKREVKDTLDSAKREAREAGDNAYAAASSAGAA
                     MDLAAYKMRPTDTNKEEAKAALKALEARIDALEDRVDAMPRGAARDAAERRVDALEDR
                     KDALESDFNKARFNALVDEVQGEWNDLVH"
BASE COUNT          235 a          403 c          332 g          163 t
ORIGIN      
        1 acgatgacga acatcatggc caagcacatg attggcgccg tgttcttcgc gatcgcgctg
       61 gttttcgtct atcgcagctt ttacagcatg cgcatccccg acgagcccac cgcggggtaa
      121 acacgccgca tcgctcgctc cggcgccact ttcaaggcgg gtcgatcgac ccgcctttct
      181 tttgcaggga cacttggcgc atcggggaaa ctgatacacc ggggaaccga gagccgcagc
      241 cgggaactat ggagctaccc cggcgcgtgc gtcgtctcgc cgcgccatcg ttgttcaacc
      301 caatcagatc tatgaaaacc aaattccttc gcacctgcct tctggcgcct acgctggctg
      361 cgttcgctgc cacccccgcg ctgctcgcga gcgaagccag cgcccgcgcc gaagcggcga
      421 tttcggaagt caaaggcgac gccgccaagg ccgccctcac cgagctcgac gccgaaatgg
      481 atcgcctcga aaccctcatc gacagcgcgc ccacgcccga ggacaaggcc gccgcgcgcg
      541 cccgtctcga cgccttgaaa gaacgccgcg ccgaactgcg gaaaacgtat gtgaaagccc
      601 gttacgacga gctgaaagcc gacgtgcgcg cggaagccaa tcgcgtcgga aactgggcga
      661 aacgcacctt caatcgcgac gaagccagca atgcgaagcg cgaagtgaag gacaccctcg
      721 actccgccaa acgcgaagcg cgcgaggccg gcgacaacgc ctacgccgcc gccagctccg
      781 ccggcgccgc gatggatctc gccgcctaca agatgcgccc gaccgacacc aacaaggagg
      841 aagccaaggc cgcgttgaaa gctctcgaag cccgcatcga tgcgctcgaa gaccgcgtcg
      901 acgccatgcc gcgcggcgcg gcgcgcgacg ccgcggaacg ccgcgtggac gcgctcgaag
      961 accgcaagga cgccctcgag agcgatttca acaaagcacg tttcaacgcg ctcgtcgacg
     1021 aagtgcaggg cgagtggaac gacttggtcc actgagccgc cgcgaacgcg ccgcgcattc
     1081 gcatccttct tctcgttcac ccgaagcgga gccagccctg gctccgcttt ttt
//
LOCUS       DASPLT010000925         1057 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_112668, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000925 DASPLT010000000
VERSION     DASPLT010000925.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1057)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1057)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1057
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_112668"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            126..509
                     /locus_tag="VEA63_09625"
     CDS             126..509
                     /locus_tag="VEA63_09625"
                     /inference="COORDINATES: protein motif:HMM:NF017868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase family protein"
                     /protein_id="HYD84303.1"
                     /translation="MPPETSPILLFIYGTLKRGCSNHRVLAGQTFLGEARTAPGYRLY
                     DLGEYPGMIAVSGDHQGVSGELWSVTPEILAQLDAFEGIPQGLYRRERIALRPPFADR
                     TVNTYFYNLSVLGRPELTHGTWTEK"
     gene            complement(610..>1057)
                     /locus_tag="VEA63_09630"
     CDS             complement(610..>1057)
                     /locus_tag="VEA63_09630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD84304.1"
                     /translation="LEAQLGAALEASVAVPEARSAAVRREALSTTNGAAAAAFPAGAE
                     EAVEEPDAVANGVVTPPMDNPDETNVWPTAAEEAAFLSDARGRGEVVVPVARETVEEA
                     EDNAKALPKLDELVEQIPTETRELLDELFRARFVAVRRVKKADLKK"
BASE COUNT          176 a          429 c          251 g          201 t
ORIGIN      
        1 caaaactccg gccgcgacaa caccaccgcc gtcgtcgtcg aaatgcttta ggtggagacc
       61 gacgtcccgt cgcgcttgac cctcctcgct cacttccgct tcccgatctt catcgaactc
      121 acacgatgcc tccggaaacc tcgcccatcc tcctttttat ttacggcacc ctcaaacgcg
      181 gttgctccaa ccatcgcgtc ctcgccggcc aaaccttttt gggcgaagcg cgcaccgccc
      241 ccggttaccg gctctacgat ctcggcgaat accccggcat gatcgctgta tctggcgacc
      301 atcaaggcgt cagcggcgaa ctctggtccg tcacccccga aattctcgcg cagctcgacg
      361 ccttcgaagg catcccgcaa ggcctctacc gccgcgaacg catcgctttg cggccgccct
      421 tcgccgatcg caccgttaac acttattttt acaacctgag cgtcctcggc cgccccgaac
      481 tgacccacgg cacctggacc gagaagtagc ggcaaatctc cacctgcttc gtccggctac
      541 cggtagttgc ctcggtagcg aaaagcgtga gcttttcggc tgtcgcacca ccggcggatt
      601 acgcgccggc tacttcttca aatccgcctt cttcacccgc cgcacggcga cgaatctcgc
      661 gcgaaacaac tcgtccagca gctcccgcgt ctccgtcggg atttgctcca ccaattcatc
      721 cagcttcggc agcgccttcg cgttgtcctc cgcctcctca accgtttcac gcgccaccgg
      781 caccaccact tcgccgcgac cgcgcgcgtc cgacaaaaac gccgcttcct ccgccgccgt
      841 gggccacaca ttcgtctcat ccggattgtc catcggcggc gtaacaacgc cgttagcgac
      901 cgcgtccggc tcttcgaccg cctcctcggc ccctgccggg aacgccgccg ccgccgcgcc
      961 attcgttgtg ctcaacgcct cccgccgcac cgcagccgat cgcgcttccg gcaccgcgac
     1021 gctcgcttcc aacgccgcgc cgagctgcgc ttccaac
//
LOCUS       DASPLT010000926         1692 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_71379, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000926 DASPLT010000000
VERSION     DASPLT010000926.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1692)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1692)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1692
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_71379"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1067)
                     /gene="ugpC"
                     /locus_tag="VEA63_09635"
     CDS             complement(<1..1067)
                     /gene="ugpC"
                     /locus_tag="VEA63_09635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HYD84305.1"
                     /translation="MAKVLIENLVKTYPEKTGPGVTAVKGISLEIQDREFMVLVGPSG
                     CGKSTTLRMIAGLEEISGGTISIDGKVVNDVLPKDRDIAMVFQNYALYPHMTVYENMA
                     FGLKLRKLPKPEIEARVREAAVMLGLEPYLDRRPKALSGGQRQRVAVGRAIVRKPKVF
                     LFDEPLSNLDAKMRVSTRTEISKLHDRLGATMIYVTHDQVEAMTMGDRICVMKDGHIM
                     QVAEPLTLYNQPANIFVASFIGSPPMNLFKGTIRRSGNALTFVETNAAATPIAVTLDD
                     TLSRKAAAYIDKPIVFGIRPENVHDTLSTADADPSRTIDVKVEVSEPMGAETYLYLDT
                     GATSFIARVRPTDRFEANQRV"
     gene            complement(1459..>1692)
                     /locus_tag="VEA63_09640"
     CDS             complement(1459..>1692)
                     /locus_tag="VEA63_09640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD84306.1"
                     /translation="VYRGGTRIATNDNWDADALLAAEISAASTAINTSQLASGSKDAA
                     LLLTLPPEIYTVHVTGGAASSGTALVEIYEITE"
BASE COUNT          296 a          420 c          628 g          348 t
ORIGIN      
        1 ttcacgcgtt ggtttgcttc gaagcgatcg gtggggcgga cgcgtgcgat gaaggacgtc
       61 gctccggtgt cgagatagag atacgtttcg gcgcccatcg gttcggagac ctcgactttc
      121 acgtcgatcg tgcgcgacgg atcggcgtcg gcggtgctga gcgtgtcgtg aacgttttcc
      181 gggcgaatgc cgaagacgat cggcttgtcg atgtaagcgg cggctttgcg cgagagggtg
      241 tcgtcgagcg tgacggcgat gggagtcgcg gcggcgttgg tctcgacgaa ggtgagcgcg
      301 ttgcctgagc ggcggattgt gcctttaaag aggttcatcg ggggcgagcc gataaagctg
      361 gcgacgaaga tattcgcggg ctggttgtag agcgtgagcg gttcggcgac ctgcatgatg
      421 tggccgtctt tcatcacgca gatgcggtcg cccatggtca tggcttcgac ctggtcgtgc
      481 gtgacgtaga tcatcgtggc gccgaggcgg tcgtggagtt tggagatttc ggtgcgcgtg
      541 gagacgcgca tcttggcgtc gaggttggag agcggttcgt cgaagaggaa aactttcggt
      601 ttgcgaacga tggcgcggcc gacggcgacg cgctggcgtt gtccgccgga gagggctttc
      661 ggtcggcggt cgagataagg ctcgaggccg agcatgaccg cggcttcgcg gacgcgggct
      721 tcgatctcgg gcttcgggag ttttcggagt ttcaggccga acgccatgtt ctcgtagacc
      781 gtcatgtgcg ggtagagcgc gtagttttgg aaaaccatcg cgatatcgcg gtctttgggg
      841 aggacgtcgt tcacgacctt gccatcgatg gagatggtgc cgccggagat ttcctcgagg
      901 ccggcgatca tgcgcagcgt ggtggatttg ccgcagccgg aggggccgac gagcaccatg
      961 aactcgcgat cctggatctc gaggctgatg cctttgacgg cggtgacgcc cgggccggtt
     1021 ttctcggggt aggttttgac gaggttttcg atgagaactt tggccatgag gtcctttcag
     1081 gggcgttcgg tgttgcggag aaacaggccg aagggtgaac gcgttcgggc ggaaaagaaa
     1141 agccgcacgt tgctgaagcg gttgaacgag gcaatgcctg acgcttgcac gtgagttgcg
     1201 cgctcttgct agaggggatt tcaagccgcg aagccgaaat cccctccagc ctgggtgatc
     1261 gcacgcgatt aatccgagtt gttacacaga gaccacggag cgccgacacg aaggaagcag
     1321 gaggccacaa ggtgtttgaa ggcctttcat gcggacgaat gctcgttgga aaaacgggcg
     1381 cgacgcggcc cagcccgagg gccgacctta caggcgctgc cgcgccatcg gggaagcgct
     1441 gcggggtagg ttgaaacgct actcggtgat ttcgtagatt tccacgagag cggtgccaga
     1501 gctcgcagcg ccaccggtga cgtgcaccgt gtagatttcg ggcgggagcg tcagcaggag
     1561 agcagcgtct ttgctgccgg aggcgagttg cgatgtgttg atcgcggtgg acgcggcgga
     1621 gatttcggca gcgagaagcg cgtcggcatc ccagttgtcg ttcgtggcga tccgggttcc
     1681 gccgcggtaa ac
//
LOCUS       DASPLT010000927         3011 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_71386, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000927 DASPLT010000000
VERSION     DASPLT010000927.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 3011)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3011)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3011
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_71386"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..741)
                     /locus_tag="VEA63_09645"
     CDS             complement(<1..741)
                     /locus_tag="VEA63_09645"
                     /inference="COORDINATES: protein motif:HMM:NF015971.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosidase"
                     /protein_id="HYD84307.1"
                     /translation="MLRFSANPVLSARDVPYPATLTFNAGVVRHDGRYLMVFRNDHGR
                     MGDESFDGTNIGLATSRDGLKWTVEPEPILVEEEVRGQFKRLLRHRYAPDFVRRIYDP
                     RLTVIEGRLFMCFAVDTAHGICGGVAETRDFKTFNWLSVSSPDNRNLVLFPRRIGGNF
                     ARLERPLPVYMRPNPEDFPIWYAESPDLVYWGRNRPVMGVDEVPFANSKIGPAAPPLE
                     TPQGWLVTFHAVRRDDSRRLKGWERKGWF"
     gene            823..1866
                     /locus_tag="VEA63_09650"
     CDS             823..1866
                     /locus_tag="VEA63_09650"
                     /inference="COORDINATES: protein motif:HMM:NF012576.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HYD84308.1"
                     /translation="MKKGSAYVTQRHVAKRAGVHQTTVSLVLRNHPSVPQETRERVLA
                     AVKQLGYKRHPFLAALMSSRMRLTSGTRNSTLAFLTDFDSRDRWKESPTAMEMFEGAR
                     TRAQELGFRIEVHWLNDPRIGPKRLADILKARNIHGLLLAPTHLPRGFFAFDFSAFSV
                     VGLGVSSETSSLLSVAHDHFNGMRRALAQCAAAGHTRVGVALTMEANQFVKDKWLAAY
                     SLDTGTGKKLARISAWQQQPFEAKAFQTWLQKNEVQAVVGTFDDKFPAALAAMGYDVP
                     GELALVSLSLTSMDRRHAGIYQRSSTIGARAVDLAVAALNHNQSGLLPMRQVMQIEGE
                     WHDGASLPAPPSS"
     gene            1950..>3011
                     /locus_tag="VEA63_09655"
     CDS             1950..>3011
                     /locus_tag="VEA63_09655"
                     /inference="COORDINATES: protein motif:HMM:NF024120.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 28-related protein"
                     /protein_id="HYD84309.1"
                     /translation="MNLFGRILLSCLACLAFLAPAGASSPPAVYTLGDDDRPSDEFTL
                     VANGAPVPVLAHTALYDYAHFSADGAIRVTVTSPEPVRTWQISPLARQIPATADGRQL
                     TFELPAAAYLIVKINDLKELALAVDPLERDVPAPAGNGIFNVTAAPYHADATGRTLVT
                     AALQQAIDDAHAAGGGVVYFPPGTFLSASLQLRSHVSLYLAGGATLRSSGDPAQFRNY
                     YHKDSLHMHGTWFVHTEPGSENIRIFGRGTIDGNARELRGQNRYLNNLVVPLQTSRFT
                     LEGVVLRDSGLWGVIPTRSDQVVIRDTKHLNEVAAFFENDAVDIIECQDVLVSRTFAI
                     SEDDTYSTKTWNEETDIAVN"
BASE COUNT          553 a         1078 c          864 g          516 t
ORIGIN      
        1 gaaccagccc ttccgctccc agcctttcaa ccgccgcgaa tcgtcccggc gaaccgcgtg
       61 aaacgtcacg agccagccct gcggcgtttc gagcggaggc gcggcaggac cgatcttcga
      121 gttcgcaaac ggcacctcgt ccacgcccat caccggacga ttgcggcccc agtaaaccag
      181 gtccggcgac tccgcatacc agatcggaaa atcttccgga ttcggacgca tgtaaaccgg
      241 caaaggacgc tccagccgcg cgaaattgcc gccgatgcgt cgcggaaaaa gcacgaggtt
      301 gcggttgtcc ggcgaactca cgctgagcca gttgaacgtc ttgaaatcgc gcgtctccgc
      361 cacgccgcca cagatgccgt gggcggtgtc caccgcgaag cacatgaata gcctcccttc
      421 gatcacggtc aggcgcggat cgtaaatccg gcggacgaaa tccggcgcgt agcggtgacg
      481 cagcagccgc ttgaactgcc cgcgcacctc ttcctcgacg agaatgggtt ccggttcgac
      541 ggtccacttg agtccgtcgc ggctggtcgc gagcccgatg ttcgtgccgt cgaatgactc
      601 gtcccccatt cgcccatggt cgttgcgaaa gaccatgaga taacggccgt cgtggcgcac
      661 cacgccggcg ttgaacgtga gcgtcgccgg ataaggaaca tcgcgggcgg acaggacggg
      721 attggcggaa aatctgagca ttgccggaga tcgctttggg gtcgcgggag gtttgcggct
      781 tgccccgcgc ctgccaaact ccgcattact cttaccgaag acatgaagaa aggctccgcc
      841 tacgtcacgc agcgccacgt cgccaagcgc gcgggcgtgc accagacgac cgtctcgctg
      901 gtgctgcgca atcatccctc cgtgccgcag gagacgcgcg agcgcgtcct cgccgcggtg
      961 aaacaactcg gctacaagcg gcatccgttt ctcgccgcgc tgatgagcag ccgcatgcgg
     1021 ctgaccagcg gcacgcgcaa ctcgactctc gccttcctca ccgacttcga ctcgcgcgat
     1081 cgctggaagg aatcgccgac cgccatggaa atgttcgagg gggcgcgcac ccgcgcccag
     1141 gagctgggct tccgcatcga ggtgcactgg ttgaacgatc cgcgaatcgg gccgaagcgc
     1201 ctcgccgaca ttctcaaggc ccgcaacatc cacgggctgc tgctcgcacc cacgcacctc
     1261 ccgcgcggtt tcttcgcctt cgacttctcc gctttctccg tcgtcggcct cggcgtcagc
     1321 agcgagacct cctcactgct cagcgtcgcg cacgatcact tcaacgggat gcgtcgcgcg
     1381 ctcgcgcagt gcgccgcggc gggccacacg cgcgtcggcg tggcactcac gatggaggcc
     1441 aaccagtttg tgaaagacaa atggctcgcc gcctattcgc tcgataccgg caccggaaaa
     1501 aaactcgccc gtatttccgc gtggcagcag cagccgttcg aggcgaaggc gtttcaaacg
     1561 tggctgcaga aaaacgaggt gcaggccgtc gttggcacct tcgacgataa attcccggcc
     1621 gccctggccg cgatgggtta cgacgtgccc ggcgaactgg ctctcgtctc gctcagcctc
     1681 acctcgatgg accgccgcca tgccggaatc tatcaacgct ccagcaccat tggcgcacgc
     1741 gccgtggatc tcgccgttgc cgcgctgaac cacaaccagt cgggcctgct gcccatgcgg
     1801 caggtcatgc agatcgaagg cgaatggcac gacggcgcgt cgttgccggc tcctccgagc
     1861 tcttagataa gagaaacgcg ggcgttgcca cgcgcacccg gctgtttccg gatggctttt
     1921 gccgcgctga gcctcatcgc gtgatccccg tgaacctctt cggtcgcatt ctcctttcgt
     1981 gtctcgcttg cctggcattt ctcgcgcccg ccggcgcaag ttctccgccg gcggtctaca
     2041 cgctcggaga cgacgaccgg cccagtgacg agttcacttt ggtcgccaac ggcgcaccgg
     2101 tgcccgtcct cgcgcacacc gcgctttacg actacgctca tttttccgcc gatggcgcca
     2161 tccgcgtcac cgtcaccagc ccggagccgg tccggacctg gcaaatcagt ccgctcgctc
     2221 gccagatccc ggcgacggcc gacggccggc agctcacttt cgaactcccg gccgccgcct
     2281 acctgatcgt caagatcaac gatctcaagg aactcgcgct cgcggtcgat ccgctggagc
     2341 gcgacgttcc cgcgcccgcg ggaaacggga tcttcaacgt caccgccgcg ccctatcacg
     2401 ccgatgccac cggtcgcacc ctcgtcaccg ccgcattgca acaggccatc gacgacgccc
     2461 acgccgcagg cggcggtgtc gtttatttcc cgcccggcac gtttctcagc gcgagcctcc
     2521 aactccgcag ccacgtctcc ctttacctcg ccggcggcgc gacactccgc agttcgggcg
     2581 atcccgcgca gtttcggaat tactaccaca aggactcgct gcacatgcac ggcacctggt
     2641 tcgtccacac cgagcccggc tccgaaaaca tccgcatctt cggacgcggc acgatcgatg
     2701 gcaacgcgcg cgaactgcgc ggccaaaacc gctacctcaa caatctcgtc gtcccgctcc
     2761 agaccagccg cttcacgctc gaaggcgttg tgctgcgcga cagcggcctg tggggcgtca
     2821 tccccacccg ctccgatcaa gtcgtgatcc gcgacaccaa gcatttaaac gaagtcgccg
     2881 ccttcttcga aaacgacgcc gtcgacatca tcgaatgcca ggacgttctt gtctcgcgca
     2941 cgttcgcgat ttcggaagac gacacctact cgacgaaaac ctggaacgaa gagaccgaca
     3001 tcgcggtaaa c
//
LOCUS       DASPLT010000928         1016 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_175570, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000928 DASPLT010000000
VERSION     DASPLT010000928.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1016)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1016)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1016
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_175570"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..918)
                     /locus_tag="VEA63_09660"
     CDS             complement(<1..918)
                     /locus_tag="VEA63_09660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD84310.1"
                     /translation="MLFVDTKTGEIITEANPVAWFEANRRETFDHLPPVEPDPETVRQ
                     SMEEYRNGDYVTSEQYIAELQKSMPEESQPSADEIEMDAVREVIQNITKPFLNDMSPS
                     EFIVYQNRQLQTADEATEKAFDRGAASRNDEISGLKSIIANCDATHAEALTRAAALEN
                     HLRTRAETAERERDEAIQKFNTALAVLAERESVYLALKKSAAEKLEHGSALEWGNAAA
                     AVRVAAYKIRCLTNTPRKPLVVTEATPVKMTVGQAVDVACELAEARAERDAARETLRT
                     LNATAAKQVADNDAEIERLRAAYAELERTC"
BASE COUNT          156 a          314 c          300 g          246 t
ORIGIN      
        1 gcaggtgcgt tccaactcgg catacgctgc ccgtaaccgc tcgatctccg cgtcgttatc
       61 ggcaacctgc ttggcggccg tggcgttgag ggttcgcagg gtttcgcggg ctgcgtcgcg
      121 ttcggcgcgg gcctcggcaa gttcgcacgc cacatcaacg gcttgaccga cggtcatttt
      181 caccggcgtc gcttcggtaa cgacgagcgg cttgcgcggc gtattggtga ggcaacgaat
      241 cttgtaggcc gcgactcgca cggctgccgc tgcgttgccc cactctaatg ccgatccgtg
      301 ctccaatttt tctgcggccg acttcttgag tgccaggtag accgactcgc gttcggccag
      361 gacggccagg gcggtgttga atttctggat tgcctcgtcc cgctcccgct ccgccgtctc
      421 cgctcgcgtc cgcaggtggt tttccagcgc tgcggctcgc gtgagggctt cggcgtgagt
      481 ggcgtcgcag ttcgcgatga tcgatttaag cccgctaatc tcgtcgttgc gggatgccgc
      541 gccgcggtca aatgcctttt cagtcgcctc gtcggcggtc tgtaactgcc tgttttggta
      601 aacaatgaac tcgctcggcg acatatcatt cagaaacggt ttcgtgatgt tctgaattac
      661 ttcgcgcacg gcgtccattt ctatttcgtc ggctgacggc tgggattctt ccggcatgga
      721 cttttgcaac tcggcaatgt attgctcgct cgtcacgtag tctccatttc gatattcctc
      781 catcgactgg cgcacggtct cagggtcagg ctccaccggc ggcaggtggt cgaaggtttc
      841 gcggcgattg gcttcgaacc aggcaaccgg gttggcctcg gtaattatct cgcctgtttt
      901 cgtgtcaaca aaaagcacgc cgtcacccgg cccacgctgt tcgaaatatg cgtcgttcca
      961 aaaaaacgac cgccaccgcc tcggataccg atgcaccttc ggcggctcgg caggct
//
LOCUS       DASPLT010000929         1753 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_11698, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000929 DASPLT010000000
VERSION     DASPLT010000929.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1753)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1753)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1753
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_11698"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1753
                     /locus_tag="VEA63_09665"
     CDS             <1..>1753
                     /locus_tag="VEA63_09665"
                     /inference="COORDINATES: protein motif:HMM:NF037085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBG domain-containing protein"
                     /protein_id="HYD84311.1"
                     /translation="TATITGFEDADDESIVSGLQFSTNAMQTTGVGVYEIMPFGATAT
                     GYEIDYEAGYLTIDRAPLTITAATVFRSYGDENPAFTASFSGLVNDDTSAVVSGLEFA
                     TDANKRSNVGAYALNVSGGSAANYAITHAPGAVNISPATLTVTADDLTRVYGDPNPAP
                     TITVTGLKNEDRLENVVHLFGPTHFATEISGIGAYGINLQGFSISANYNATVRSGSLT
                     ITQRPLTIVADSKTKVYGDPNPEFTATFRGLASFDTAAVIPNVRLSTQATQTSGVSTF
                     GILVTSDLSRNYKIGYEFGQLKITPAELGVTGLTDLLRTYGRADPTLPRIEVFGLKNS
                     DTVSMLGLEFVLPAPTVDAGEYKYSIIARNSNYTLLGNEAYFIVEPAPLALRVTPMGR
                     LYGDANPSIAFDLAGLAFGQTADQVLRIDNPTDATTDVGTYNLFPELLTNNYVLTDIR
                     GNTIRINPRLLTVDVDNHARYYGDATTEFTYVLGGDGLAPLDSPFAVFEEVRTAVPIN
                     EQTNVGWYRIDPIFTNNPNYRVSWSPGYLAIMPRPIEITVHNGVSFGNNNVPEDFDAE
                     TLGLNLILFDPNGSGGLG"
BASE COUNT          352 a          503 c          569 g          329 t
ORIGIN      
        1 acggcgacga tcacaggctt cgaagacgcc gacgacgagt cgatcgtgtc cggcctgcag
       61 ttcagcacga atgcgatgca gactaccggc gtgggcgttt atgaaatcat gccctttggt
      121 gcgacggcga cgggctacga aatcgactac gaggcgggtt atctcacgat cgatcgggcg
      181 ccgttgacga tcacggcggc gacggtgttc cggagttacg gcgacgagaa tccggcgttc
      241 acggcgagct tttcggggct ggtgaacgat gacacgagcg cggtggtgag cgggctggag
      301 ttcgcgacgg atgcgaacaa gcggtcgaat gtcggcgcgt atgccttgaa cgtcagcggc
      361 ggcagcgcgg cgaattatgc gatcacgcat gcacccggcg ccgtgaatat ttcgccggcg
      421 acgctgacgg tgacggcgga cgacctgacg cgcgtttacg gcgatccgaa cccggcgccg
      481 acgatcacgg taaccggctt gaagaacgag gatcggctgg aaaacgtggt gcacctgttt
      541 ggaccgacgc atttcgcgac agagatctcg ggcatcggcg cgtatggcat caatctccag
      601 gggttctcga tctcggcgaa ttacaacgcg acggtgcggt ccggctcgct gacaatcacg
      661 cagcggccgc tgacgatcgt ggcggacagc aagacgaagg tttacggcga tccgaatccg
      721 gaattcaccg ctaccttccg cggactggcg tcgttcgaca cggcggcggt gattccgaat
      781 gtgcggttgt cgacgcaggc gacgcagacc tcgggcgtga gcacgttcgg tattctggtg
      841 acctcggatc tcagccggaa ttacaaaatc ggctacgaat tcgggcagtt gaagatcacg
      901 ccggcggaac tcggcgtcac gggcttgacc gaccttttgc gcacctacgg ccgcgcggac
      961 cccacgctgc cgaggatcga agtttttgga ctcaagaatt cggatacagt ttcgatgctc
     1021 ggcctcgagt tcgtgctgcc ggcgccgacg gtggacgcgg gggaatacaa atattccatc
     1081 atcgcgcgga actcgaacta cacgctgctg ggcaacgagg cctatttcat cgtcgaaccc
     1141 gcgccgcttg cgttgcgcgt gacgcccatg gggcgccttt acggcgatgc gaatccgagc
     1201 attgcgttcg atctcgcggg gctggctttc ggccaaacgg ccgaccaagt gctgcggatc
     1261 gacaacccga cggacgcgac gacggacgtc ggcacctaca atctttttcc cgagctgctg
     1321 acgaacaact acgtgctgac ggatatccgc ggaaacacga ttcgaatcaa cccgcgcctg
     1381 ctcacggtcg acgtggacaa tcatgcgcgt tattacggcg atgcgacgac ggagttcacc
     1441 tatgtgctgg gcggcgatgg actggcgccg cttgatagcc cgttcgcggt gttcgaggag
     1501 gtgcggacgg cggtgccgat caacgaacaa accaacgtgg gctggtatcg gatcgacccc
     1561 atcttcacca acaatccgaa ttatcgggtg tcatggagtc ctggatacct ggcgatcatg
     1621 ccgcggccga tcgagatcac ggtgcacaac ggcgtgtcgt tcggaaacaa caacgtgccg
     1681 gaggatttcg atgccgaaac gttggggcta aacctgatcc ttttcgatcc gaacggttcg
     1741 ggcgggctcg gct
//
LOCUS       DASPLT010000930         1198 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_215104, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000930 DASPLT010000000
VERSION     DASPLT010000930.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1198)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1198)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1198
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_215104"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..493
                     /locus_tag="VEA63_09670"
     CDS             <1..493
                     /locus_tag="VEA63_09670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HYD84312.1"
                     /translation="QEYGRYFNLPCCCLRGGCLTGPNHSGVELHGFLSYLVKCNLEGR
                     EYKVFGYKGKQVRDNIHSLDVARFMAAFVAAPRAGEVYNLGGGKANSTSILEAFKLTE
                     KFTGRAQVYSYVDQNRIGDHICYYSDLRKMRAHYPSWDITQSLEETIRQIVEAWQRRA
                     KSA"
     gene            591..>1198
                     /locus_tag="VEA63_09675"
     CDS             591..>1198
                     /locus_tag="VEA63_09675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD84313.1"
                     /translation="MILRPFRTCLVLAFVGVSIFSAHAMSVQPPSFDELVAEAHTVTR
                     ARVKHVETRFVIAPDGQRLLKTFVTFSKLKTLKGAAPEEFTLEFLGGEKDGERWTIPG
                     MPQFESGDEEFLFPTAGTSICPLVGAMHGRYRVLTDPATQRRYVARDNLEPLASTDDV
                     ARPIDPDSPTVRVATDTAHALTPDEFERRIVDHVKRPSRPKPA"
BASE COUNT          213 a          439 c          323 g          223 t
ORIGIN      
        1 tcaggaatac ggccgctact ttaatctccc ctgctgctgc ctccgcggcg gttgtctcac
       61 cggtccgaat cactccggcg tcgagctgca cggcttcctt tcctacctcg tgaaatgcaa
      121 cctcgagggc cgcgaataca aagttttcgg ctacaagggc aaacaggtgc gcgacaacat
      181 ccactcgctc gacgtcgcac gcttcatggc cgcgttcgtc gccgccccgc gcgctggcga
      241 ggtttacaat ctcggcggcg gcaaggccaa cagcacgtcc atcctcgaag cgttcaagct
      301 cacggagaaa ttcaccggcc gcgcgcaggt ctacagctac gtcgaccaga accgcatcgg
      361 cgaccacatc tgctactact cggacctccg caagatgcgt gcgcattacc cgtcgtggga
      421 catcacgcag tccctcgagg aaaccatccg ccagatcgtc gaagcctggc agcggcgcgc
      481 gaaatccgcc tgaggttccc gccttcgtgg cgggtttttt acgtaacatt cgccggctcg
      541 gagctgtcct gaggtgcgca ggtgagtagc ctgcgcatct ctcgtctctc atgattctgc
      601 gtccgttccg cacctgcctt gtcctcgcct tcgtcggcgt atccatattt tccgcacacg
      661 cgatgtcggt tcagccgccg tcgttcgacg aactcgtcgc ggaagctcac actgtcacgc
      721 gcgctcgcgt caaacacgtc gagacgcgct tcgtcatcgc tcccgacggc caacgcctgc
      781 tgaaaacctt cgtcacgttc tccaagctca aaacgctcaa gggcgccgcg cccgaggagt
      841 tcacgctcga gttcctcggc ggcgaaaagg atggcgagcg ctggacgatt cccggcatgc
      901 cgcaattcga atccggcgac gaagagtttc tcttccccac cgccggcacc tcgatctgcc
      961 cgctcgttgg cgcgatgcac ggtcgctatc gcgtgctcac cgatcccgcc acgcagcgcc
     1021 gctacgtcgc gcgcgacaac ctcgagccgc tcgccagcac cgacgacgtc gcccggccga
     1081 tcgatcccga ttcacccacg gtccgcgtcg ccaccgacac cgcgcacgcg ctcacgcccg
     1141 acgaattcga acgccgtatt gtcgatcacg tgaagcgccc gtcccgtccg aagccggc
//
LOCUS       DASPLT010000931         1018 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_175603, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000931 DASPLT010000000
VERSION     DASPLT010000931.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1018)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1018)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1018
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_175603"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..234)
                     /locus_tag="VEA63_09680"
     CDS             complement(<1..234)
                     /locus_tag="VEA63_09680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD84314.1"
                     /translation="MKRRAMRWVSQRTGFAFVALGLGVLGFAAGCGKKMEPAAVAGEE
                     AKHAGHEHGHGHEHHAPHGGVVVVLGGEAYHLEL"
     gene            complement(231..782)
                     /locus_tag="VEA63_09685"
     CDS             complement(231..782)
                     /locus_tag="VEA63_09685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007361990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD84315.1"
                     /translation="MKTHSRLLSWTGVAALLLHVVVVVAQDAPAVDLTPRKVVVAGEE
                     MLALGFDKLSSFTYTLVDAGTGATEEEIAEHLKKDPVPEWVRAYDSKRVLLTGYMMPL
                     QVENGRSKKFVMMRDVNTCCYGAVPNMNDYLVVTMEGEGVEAIQDVPVDLVGVFKIDH
                     RYDGGYVVSLFAMKGEKFLGPKK"
     gene            complement(793..>1018)
                     /locus_tag="VEA63_09690"
     CDS             complement(793..>1018)
                     /locus_tag="VEA63_09690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009509793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HYD84316.1"
                     /translation="LLGMVIAFVIYGVIVGVASGIIRAQTGVVINPFELHPVMLAAPA
                     GIVALAALCGIFPALKAYRTDVAASISPTS"
BASE COUNT          220 a          376 c          242 g          180 t
ORIGIN      
        1 caattcgaga tgatacgcct cgccgcccag cacgacgacc accccgccgt gcggcgcgtg
       61 atgctcgtgc ccatgcccgt gctcatgtcc cgcgtgcttc gcctcttctc cggcaaccgc
      121 agccggctcc attttcttcc cgcaacccgc cgcaaatccc aacacgccca aaccgagcgc
      181 aacgaacgca aaccccgtcc gctgactcac ccatcgcatc gcacgtcgct tcatttcttc
      241 gggcccaaaa atttttcacc cttcatcgcg aacagcgaca cgacgtagcc gccgtcgtag
      301 cggtgatcga ttttgaacac ccccacgaga tcgaccggca cgtcctgaat cgcttcgacg
      361 ccctcgcctt ccatcgtgac cacgaggtaa tcgttcatgt tcggcaccgc gccgtagcag
      421 caggtgttca catcccgcat catcacgaat ttcttcgacc gcccgttctc cacctgcagc
      481 ggcatcatgt agccggtgag caacacgcgc ttgctgtcat aggcgcgcac ccactccggc
      541 accggatctt tcttcagatg ctccgcaatc tcttcctccg tcgcaccggt gccggcgtcg
      601 accagcgtgt aggtaaacga cgacagcttg tcgaaaccga gcgccagcat ctcctcaccc
      661 gccaccacca cctttcgcgg cgtcaaatca accgccggcg cgtcctgggc aaccacgaca
      721 acaacgtgga gaagcaaagc cgcgacacca gtccaactca acaaacgaga atgtgttttc
      781 atgatgatcg attcacgagg tcggtgaaat gctggcggcc acatccgtgc gataagcctt
      841 cagcgcagga aaaattccgc aaagcgccgc cagcgccacg atccccgccg gcgccgcgag
      901 catgaccgga tgaagctcga atgggttgat caccacgccc gtttgcgcgc ggataatccc
      961 cgacgccacg ccgacaatta ccccgtagat cacaaacgcg atcaccatgc cgagaagc
//
LOCUS       DASPLT010000932         2893 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_11727, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000932 DASPLT010000000
VERSION     DASPLT010000932.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 2893)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2893)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2893
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_11727"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            231..1361
                     /locus_tag="VEA63_09695"
     CDS             231..1361
                     /locus_tag="VEA63_09695"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HYD84317.1"
                     /translation="MRVCEIVPSLEERYGGPSKSVLALSRALAANDNDVALLTTHPAS
                     ASQQSDGRFSQHVFRRDWPGTLCPSIDLRAHLDASACDVVHHHSIWLRTLHYAHRRAR
                     SARVPLVISPRGMMSAWAWNHHAARKQLARSFVHPGALEAAAGWHATSTEEAAEIRAL
                     GFKQPVCVAPNGVSAPTDAERAIAIAHWREACPDVARRPTAVFYSRLHRKKRVLELIE
                     LWLARAPADWLLLVVGLPEDYTPAQLETFVTRASGGGRVRIFDGTARPAPYAVASLFL
                     LPSHNENFGLVIAEAMAHGVPALVTDATPWTSLNAAGAGWCVPWSAYGDALAAALAEG
                     PDALDARGTVARTHVLANYSWSKSAEIVNGFYHELSARRKTA"
     gene            1358..2851
                     /locus_tag="VEA63_09700"
     CDS             1358..2851
                     /locus_tag="VEA63_09700"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HYD84318.1"
                     /translation="MSEHDQPRPRSRSRSTNAEQPLARLELATLAHVGVLVVFAAWWL
                     GGNTLLARTLLSIWGTLGVAITVAAALKRGQSSDRLRPLRWLWPLALFNAMVIASCFN
                     PSFREVTDETRNFFVEDRARLLLPSTARPLHTLQTLWFFDAVYLSCFNLLLVVRQRRA
                     LRALLLILAGNALLLAVFGTAQKLVGAKGLFFGFIDSPQVYFFSSFIYHNHWGAFTVL
                     MTALSLGLVAHFARRSASRDFWHSPVPSGLVAILLLAASIPLSASRSSTVLILLLLAI
                     GLVHWLWRFLRRRRGGLTEKSTLLPLVAAIAALGLGGAFAYTLARPVIEHRLEITQEQ
                     IAQMRSRGDFGSRAILYRDTWNMAREKPWFGWGMGAYPTAFFSRNTQQTSPIDGLPQY
                     YHDAHSDWLQSASEVGLIGTTLLGLCALVPLWGSRRGLTQSPLPIYLLIGCGSVVLYA
                     ALEFPFGNRAVICVWWTCFFSALHYARADRASTGSADPADSRSARLS"
BASE COUNT          427 a         1067 c          794 g          605 t
ORIGIN      
        1 atccacgtcc atcctggtta aaaagatgtt gatcgggaat tcgtgtctat tcgtgtccat
       61 tcgtggttca aaattctggc tcctattccc tagactagcc ttggaatctg tgttcatccg
      121 tgtccatccg tggttcaaaa atttcggctc gatcggtcac cggtcgccgc ggacgcagtc
      181 gatttagccc acgttatacc gtggttttcg tctgactctt cctcgctgaa atgcgtgtct
      241 gcgaaatcgt gccaagcctc gaggaacgct acggcggacc gagcaaatcc gtcttggcgt
      301 tgtcccgtgc gctcgccgcc aacgacaacg acgttgccct gctcaccacg catcccgcat
      361 cggcttcgca gcaaagcgac ggccggttta gccagcatgt tttccgccgc gactggcctg
      421 gcacgctgtg cccgtccatc gacctccgcg ctcatctcga cgcgtccgcg tgcgacgtcg
      481 ttcaccatca ctcgatctgg ctgcgcaccc tccactacgc gcatcgccgc gcccgctccg
      541 cgcgagtgcc gctcgtgatt tcgccgcgcg ggatgatgag cgcctgggct tggaatcatc
      601 acgcagcgcg aaaacaactc gctcgctcgt tcgtgcatcc cggcgctctg gaagccgccg
      661 ccggctggca cgccaccagc accgaggaag ccgccgagat tcgcgcgctc ggtttcaagc
      721 agcccgtgtg cgtagcgccg aatggcgttt ccgcgcccac cgatgccgaa cgcgcgatcg
      781 cgatcgccca ctggcgcgaa gcctgtcccg acgtcgcgcg ccgccccacc gccgttttct
      841 attcgcggct ccatcgcaag aaacgcgtgc tcgaactcat cgaactctgg ctcgcgcgcg
      901 ctcccgccga ctggttgctg ctggtcgtcg gcctgcctga agactacacg cccgctcagc
      961 tcgagacctt tgtcacgcgc gcatccggcg gcgggcgcgt gcggatcttc gatggcacgg
     1021 cgagacccgc accctacgcc gtcgcttcgc tttttcttct gccctcgcac aacgaaaact
     1081 tcggcctcgt catcgccgaa gccatggcgc acggcgtgcc cgccctcgtg accgatgcca
     1141 cgccatggac ttcgctcaac gccgccggcg ccggctggtg cgtgccatgg tcggcctatg
     1201 gcgacgccct cgcggccgct cttgccgaag gacccgacgc gctcgacgct cgcggcaccg
     1261 tggcgcgcac gcatgtgctc gcaaactact cgtggtcgaa atccgccgaa atcgtgaacg
     1321 gcttttacca cgagctttcc gcccgccgaa aaaccgcatg agcgaacacg accaaccgcg
     1381 gcctcgaagc cgatctcgtt cgaccaacgc cgaacagccg ctcgctcgac tggagctcgc
     1441 gacgctggcg cacgtcggcg tgctcgtcgt cttcgccgcg tggtggctcg gcggaaacac
     1501 gcttctcgcc cgcaccctgc tctcgatctg gggcaccctc ggcgttgcga tcaccgtcgc
     1561 cgccgcgctt aaacgcggcc agtcctccga tcgactccgc cccctgcgct ggctctggcc
     1621 gctcgcgttg ttcaacgcga tggtcatcgc gagctgcttc aatcccagct ttcgcgaggt
     1681 gacggacgag acgcgcaatt tcttcgtcga agaccgtgca cgcctgttgc tcccaagcac
     1741 cgcgcgcccg cttcacacgc tgcaaacgct gtggtttttc gatgccgttt atctctcgtg
     1801 cttcaacctg ctgctcgtcg ttcgccagcg ccgcgcgctg cgcgccctcc tgctgatcct
     1861 cgccggcaac gccctgctgc tcgccgtttt cggcaccgcc caaaaactcg tcggcgcgaa
     1921 gggccttttc ttcggtttca tcgactcgcc gcaggtttat tttttctcgt ccttcatcta
     1981 ccacaatcac tggggcgcgt tcaccgtgct catgaccgcc ctgtcgctcg gattggttgc
     2041 gcacttcgcg cgccgcagcg ccagtcgcga tttttggcac tcgcccgtgc cgagcggact
     2101 cgtcgcgatt ctcctcctcg ccgcatcgat cccactcagc gcctcgcgct cgtccacggt
     2161 gctcattctt ctgctgctcg cgatcgggct ggtccactgg ctctggcgtt tcctgcgtcg
     2221 tcgtcgcggc ggcctgacgg aaaaatccac gttgctgccg ctcgtcgccg ccatcgccgc
     2281 gctcggcctc ggtggcgcgt tcgcttacac gctcgcgcgt cccgtgatcg aacaccgtct
     2341 cgagatcacg caggagcaaa tcgcgcagat gcgctcgcgc ggcgatttcg gctcgcgagc
     2401 catcctctat cgcgacacgt ggaacatggc ccgcgaaaag ccctggttcg gctggggcat
     2461 gggcgcttat ccgacggcgt ttttcagccg taacacgcaa cagacttcac ccatcgacgg
     2521 cctgccgcag tattaccacg acgcgcattc ggactggctg caatcggcct ccgaagtcgg
     2581 cttgatcggc accaccttgc tcggtctttg cgccctcgtg ccgctctggg gcagtcgacg
     2641 cggcctgacg caaagccccc tcccaatcta tcttctcatc ggctgcggca gcgtcgtgct
     2701 ctatgccgca ctggaatttc cttttggaaa ccgcgccgtc atctgcgtct ggtggacctg
     2761 ctttttctcc gcccttcact acgcgcgcgc cgaccgcgct tccaccggtt cggctgatcc
     2821 cgccgattcc cgctccgccc gtctctcatg agcgaacgct gccccatctc cgttctcatt
     2881 cccgcgaaaa acg
//
LOCUS       DASPLT010000933         1161 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_11734, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000933 DASPLT010000000
VERSION     DASPLT010000933.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1161)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1161)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1161
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_11734"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(407..>1161)
                     /locus_tag="VEA63_09705"
     CDS             complement(407..>1161)
                     /locus_tag="VEA63_09705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HYD84319.1"
                     /translation="GPTGSGKSTSLNAFLRKINSTDLRIITIEDPIEYEVPGVDQMQV
                     RPEIGLTFADALRHVLRQDPDVIMVGEIRDKDTAEIAIRASLTGHLVFSTLHTNDAPG
                     AITRLVDMGIEPFLVASAIELVIAQRLVRRLCSECSRPDPINKIKLRESLAILGVTEA
                     EADLVDALKAPVGCDRCRGTGYRGRIGIFEIFRLNDEMHELVLKRESTRTLTECARRH
                     GMRTLGQSGWEKVKAGYTTLDEVLRVITVAEK"
BASE COUNT          207 a          303 c          410 g          241 t
ORIGIN      
        1 ggtttttcgc gaagctggat accttgcttt gtttttcgcg ccttcagcac ttcgaggttc
       61 gagcgaggct ttccttcttg gagagacgac agaacggcaa gggccgcgga acgttgcttg
      121 aagagtgtgc cgccagaggg atcgttcatc gcgaagcagc cggattcgac gttgtaataa
      181 agcacaaaac ggcgagtgaa cgccgaggcg acgagatagc gtgtaggatg tcgagctccg
      241 ccgtgattct tttcagctca gccagttcgg actttgacgg cggcgggact tcttcgtggc
      301 cggcgtgaac gtgacggtga cgcctcggac tcgtcttctt cacagatccg gacgatggac
      361 gtggcttgac gatatcggga gctgatcggc gggggtgagg catggctcac ttctcggcga
      421 ctgtaatgac gcggagcact tcatcgagcg tggtgtagcc ggccttcact ttttcccagc
      481 cgctttggcc gagggtgcgc atgccgtggc ggcgggcgca ttcggtgagc gtgcgcgtgc
      541 tttcgcgctt caacaccaac tcgtgcattt cgtcgttgag gcggaagatt tcgaagatgc
      601 cgatgcggcc gcgataaccg gtgccgcggc agcgatcgca gccgacgggc gctttcaatg
      661 cgtcgacgag atcggcctcg gcttcggtaa cgccgaggat cgcgaggctc tcgcgcagtt
      721 tgatcttgtt gatcggatcg ggccgcgagc attcggagca gaggcggcgg acgagacgtt
      781 gcgcgatgac gagctcgatc gcggacgcga cgaggaacgg ctctatgccc atgtcgacga
      841 gacgcgtgat ggcgccgggc gcgtcgttcg tgtgaagcgt ggagaacacc aagtgaccgg
      901 tgagcgaggc gcggatggcg atctcggcgg tatccttgtc gcgaatttcg ccgaccatga
      961 tgacgtcggg atcctggcgg agaacgtggc ggagcgcgtc ggcgaacgtg aggccgatct
     1021 cggggcggac ctgcatctgg tccacgccgg gaacttcgta ctcgatcgga tcttcgatcg
     1081 tgatgatgcg aagatcggtg gagttgatct tgcggaggaa ggcgttaagc gaggtgcttt
     1141 tgccggaacc cgtaggaccg g
//
LOCUS       DASPLT010000934         1214 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_11735, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000934 DASPLT010000000
VERSION     DASPLT010000934.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1214)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1214)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1214
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_11735"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            98..592
                     /locus_tag="VEA63_09710"
     CDS             98..592
                     /locus_tag="VEA63_09710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012377033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory CII family protein"
                     /protein_id="HYD84320.1"
                     /translation="MQAHEVMREVLKRTSAKQISADMGLSLSLIYKWAEPPENDSGSG
                     ASSPLDRVGQLVRTTQDTRIAQWVCEQAGGFYIRNPHNLPAGGPLIPVTNDIVQEFAD
                     MLATIATAAADDKVTKEEAKKIRARWEELKSVTEGFVRAAEAGTFGGAEKEAEKKSAA
                     EERR"
     gene            complement(657..>1214)
                     /locus_tag="VEA63_09715"
     CDS             complement(657..>1214)
                     /locus_tag="VEA63_09715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD84321.1"
                     /translation="SHQVSGDSDPEIDFYGAYSFSDDSERWSVVPAFNLYTYPDAKRS
                     DGLYRATFEPSLSVSYTASGIRFTPTVYHDFMLRGTTAELTAEFALPLTSLGTELDFA
                     ATGGTFKWKSVTADASPDEKNWGDYWTVGVTMPFTVGARSSVSLGVLYSEGHDNFYKV
                     GTDPKQPNDDAGGHFAVTLSYTVEL"
BASE COUNT          239 a          325 c          449 g          201 t
ORIGIN      
        1 cgggttggca cgaaaaaaag gcggggtatt ttccggatcg ccggcgcggg cgagacgcgg
       61 gattgcgttt caaccgcggc ttggcaggat ccatgcgatg caagctcacg aggtgatgcg
      121 cgaagtgctg aagcgcacga gtgccaaaca aatttcagct gacatggggt tgtcgctttc
      181 cttgatctac aagtgggcgg agccgccgga aaacgacagc ggcagcggcg cgagcagtcc
      241 gctcgatcgc gtcgggcagc tcgtccgcac gacgcaggac acgcgtatcg cgcagtgggt
      301 ttgcgagcag gcgggcgggt tctacatccg caatccgcac aacctgcctg cgggcggacc
      361 gctgattccg gtgacgaacg acatcgtgca ggagttcgcc gacatgctgg cgacgatcgc
      421 gaccgccgcc gccgacgaca aggtcacgaa ggaagaggcg aagaaaattc gtgcgcgctg
      481 ggaagagttg aagagcgtga ccgaaggttt cgtgagagcg gccgaagcgg ggacgttcgg
      541 cggcgccgag aaggaagcgg aaaagaaaag cgcggcggaa gagcggcgct gagcgcgccg
      601 ggcgaggggc ggggaagcga cgttcgcgcg cgcctgcgct gcccgtgcgg cgaaggctag
      661 agttcgacgg tgtagctcag cgtgacggcg aagtggccgc cggcgtcgtc gttcggctgt
      721 ttcggatcgg tgccgacctt gtagaagttg tcgtgccctt ccgaatacag gacgccgagg
      781 ctgacgctcg aacgtgcgcc gacggtgaat ggcatcgtga cgccgacggt ccagtagtca
      841 ccccagtttt tttcgtcggg cgacgcgtcg gcggtgacgg acttccattt gaacgtgccg
      901 ccggtggcgg cgaaatcgag ctcggtgccg aggctggtga gcggcagtgc gaactcggcg
      961 gtgagctcgg cggttgtgcc gcgcagcatg aaatcgtgat agaccgtcgg cgtgaagcga
     1021 atgcctgacg cggtgtagct cacggaaagg ctcggctcga aggtcgctcg ataaaggccg
     1081 tcgctgcgct tggcgtccgg ataggtgtag agattgaagg ccggcacgac gctccagcgt
     1141 tcactgtcgt cggaaaatga atacgcgcca tagaaatcga tttcgggatc ggagtcgccc
     1201 gagacttggt ggga
//
LOCUS       DASPLT010000935         1629 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_175638, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000935 DASPLT010000000
VERSION     DASPLT010000935.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1629)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1629)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1629
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_175638"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(50..994)
                     /locus_tag="VEA63_09720"
     CDS             complement(50..994)
                     /locus_tag="VEA63_09720"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HYD84322.1"
                     /translation="MSRPPAVTVLIPVFNEQAVLPQLFQRLAAVFDANPAIAWSALLV
                     DDGSRDASATLAAAQHARDPRFRLVQLSRNFGFQNALAAGLSLSGDADAVVTMDADLQ
                     DPPEAIPQLIAAWQGGAEVVRATRRTRAERGLRRIGFDLFHACYGRLTDYPIEPNSGT
                     FGLLGRDAVAAFNQLPERNRFFPGLRAWVGFTRADVDYDRQERAAGQPQQTLRKLVHY
                     ALDGLFSFSRLPLRLLTYFGLFVAFIGFAIGVFFAVRRVLGIETAQTGFTTLVTLLLF
                     IGGVQLIGIGVLGEYLGRVYDEVKQRPNYIIKKRIGLG"
     gene            complement(1067..>1629)
                     /locus_tag="VEA63_09725"
     CDS             complement(1067..>1629)
                     /locus_tag="VEA63_09725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD84323.1"
                     /translation="RRGHVLRLPFLAWLSSLPLGIPAMMTAGGTINSFQGFVLLLPPV
                     LVLLGTAALRRRSWFPAAAALSLALIGTRWAAQPNRPLLPATTALIQADQISRLYPAK
                     IWFPWNPTVTYFSDGRFYHAEDGIYVRMLTGHRLSWRQLEQGLPSDIQYVALFGQNWG
                     IAKSFAHGSSFEQQLGYWELWRWKER"
BASE COUNT          358 a          450 c          572 g          249 t
ORIGIN      
        1 cgcgagcaaa ccggcggcga tccacgcgag cgcgcgctgc tggcgcggtt cagccaaggc
       61 caatcctctt ttttatgatg tagttcgggc gctgtttcac ctcgtcgtaa acgcgtccga
      121 ggtattcgcc cagcaccccg atgccgatga gttggacgcc gccgatgaac agcagcagcg
      181 tgaccaaggt ggtgaatccc gtttgggctg tttcaattcc gagcacgcgg cgcaccgcga
      241 agaacacgcc gatcgcgaaa ccgatgaagg caacgaagag gccaaaataa gtaagcaggc
      301 ggagcggcag gcgcgaaaaa ctgaacagac cgtcgagagc gtaatgcacg agtttccgca
      361 acgtttgctg cggctgcccg gccgcccgtt cctggcgatc atagtcaaca tcggcgcggg
      421 tgaagcctac ccaggcacgc aggccaggga agaaacgatt gcgctcgggc agttgattaa
      481 acgcggcgac ggcatcgcga ccaaggagac caaacgtgcc ggagttgggc tcgatcgggt
      541 aatcggtgag gcgtccataa cacgcgtgga ataagtcgaa gccgatgcgc cgaagcccgc
      601 gctccgcgcg cgtgcggcgg gtggcgcgca cgacctcggc gccgccttgc caggcggcga
      661 tgagttgcgg aatggcttcg ggtggatcct gaagatcggc gtccatggtc accacggcgt
      721 cggcgtcgcc ggaaagtgag aggccggcag cgagggcgtt ttgaaaaccg aaattgcgcg
      781 agagttggac gagacgaaat cgaggatcgc gagcatgctg tgcggcggcg agcgtcgcgg
      841 acgcgtcgcg gctgccgtcg tcaaccaaca gggcggacca agcgatcgcg gggttcgcgt
      901 cgaacacggc tgcgagccgc tggaagagct ggggcagcac ggcttgttcg ttgaaaaccg
      961 gaatgagaac cgtgacggcg ggcgggcggg acacggagtg tgggtatgag gaaatcggcc
     1021 gaggggtcaa agctggaggc aagggcgcca cgcccggcgc aacgttttac cgttctttcc
     1081 agcgccagag ttcccaatag ccgagctgct gctcaaagct agagccgtga gcgaaggatt
     1141 tcgcgatgcc ccagttctgg ccgaatagcg cgacatactg aatatcggag ggcaagcctt
     1201 gctcgagctg gcgccaggag aggcggtggc cggtcagcat acgcacataa atgccgtcct
     1261 cggcgtgata aaagcggccg tccgaaaaat aggtgacggt cggattccaa ggaaaccaga
     1321 tcttcgcggg atagagtcgg gaaatctgat cggcttggat caacgcggtg gtagccggca
     1381 ggagtgggcg attcggctgc gcggcccagc gggtgccaat taacgcaagg ctcagcgctg
     1441 ctgcggctgg aaaccagctg cggcgtcgca gtgcggcggt tcccagcaag acgagaacag
     1501 gcgggaggag caggacgaag ccttgaaacg aattgatcgt tccgccggcg gtcatcatcg
     1561 cgggaattcc gagcggtaga ctggaaagcc aagcgaggaa ggggagtcgc aacacgtgcc
     1621 cgcgccgca
//
LOCUS       DASPLT010000936         1165 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_196370, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000936 DASPLT010000000
VERSION     DASPLT010000936.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1165)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1165)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1165
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_196370"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1165)
                     /locus_tag="VEA63_09730"
     CDS             complement(<1..>1165)
                     /locus_tag="VEA63_09730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase central domain-containing
                     protein"
                     /protein_id="HYD84324.1"
                     /translation="TTEQLEQYFPDLKHPSMESALALVHSRFSTNTFPSWDRAHPYRY
                     IAHNGEINTLRGNINWMHARQALFQSELFGEDIQKILPIINPNGSDSSMFDNTLELLV
                     LAGRPLAHAMMMMIPEPWSNHESMDDDRRAFYQYHSCLMEPWDGPAAVAFTDGKQMGA
                     CLDRNGLRPSRYYVTKDGLVVMASEAGVLDNIPADQIAHKGRLQPGHMFLVDTEQGRI
                     IEDEEIKRQLATEHPYRQWLKEHLVKLEQLPEAPAIPPPDHDTLLHRQIAFGYTFEDQ
                     RIILAPMARDGVEAIGSMGNDTPLAVLSNKPRLLYDYFKQLFAQVTNPPIDCIREELI
                     TSAETRLGSEGNLLNPDPLACRRVELKWPVLTNEEFAKLRRLDQPGFKVGVLPI"
BASE COUNT          214 a          310 c          408 g          233 t
ORIGIN      
        1 gaatcggaag cacgccgact ttgaagccgg gctgatcgag gcggcggagt ttcgcgaact
       61 cctcgttcgt gagcacgggc cacttcagct cgacacgacg acaggcgagc gggtcgggat
      121 tgaggaggtt accctcggaa ccgagacgcg tctcagccga agtgatcaac tcctcgcgaa
      181 tgcagtcgat cggaggattg gtgacctgcg cgaagagctg cttgaaataa tcgtagagca
      241 ggcgcggctt gttcgaaagc acggcgagcg gcgtgtcgtt gcccatggag ccgatcgctt
      301 cgactccatc gcgcgccatc ggagcgagga tgatgcgctg gtcttcgaac gtgtaaccaa
      361 acgcgatctg ccggtgcagc agcgtgtcgt gatcgggcgg cggaatggcc ggtgcttcgg
      421 ggagctgctc gagtttgacg aggtgctcct tgagccattg gcgatacgga tgctcggtgg
      481 cgagctggcg cttgatttct tcgtcctcga tgatgcgacc ctgctcggta tcaacgagga
      541 acatgtgacc cggttggagg cggcccttgt gcgcgatctg atcggcgggg atgttatcca
      601 gcacacccgc ttcggaggcc atgacaacga gtccgtcttt cgtgacgtag taacgcgaag
      661 ggcgaagacc attgcggtcg aggcacgcgc ccatctgctt gccatcggtg aacgccaccg
      721 cggcggggcc atcccatggc tccatcaagc aagagtgata ctgatagaac gcgcggcggt
      781 cgtcgtccat cgactcgtgg ttggaccacg gctcggggat catcatcatc atcgcatgcg
      841 cgagtgggcg gccggcgagc acgagaagtt cgagcgtgtt gtcgaacatc gacgagtcgg
      901 agccgttcgg gttgatgatc gggaggatct tttggatgtc ctcgccgaag agctcgcttt
      961 ggaagagcgc ctggcgggcg tgcatccaat tgatgttgcc gcggagcgtg ttgatttcgc
     1021 cgttgtgcgc gatgtagcga tacgggtgtg cgcggtccca gctcgggaag gtgttcgtgg
     1081 agaaacgcga gtggacgagc gcgagcgcgg actccatcga cgggtgcttc aggtcgggga
     1141 aatactgctc gagctgctcg gtcgt
//
LOCUS       DASPLT010000937         1399 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_154475, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000937 DASPLT010000000
VERSION     DASPLT010000937.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1399)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1399)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1399
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_154475"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1352
                     /locus_tag="VEA63_09735"
     CDS             <1..1352
                     /locus_tag="VEA63_09735"
                     /inference="COORDINATES: protein motif:HMM:NF019225.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="autotransporter-associated beta strand
                     repeat-containing protein"
                     /protein_id="HYD84325.1"
                     /translation="SYIGSGLGNTGTVVMSGGTLNVGLVATPGVRADFEVGSFNGSGT
                     MTQTGGDVVLNTTGGFFHIGNQGTGVYNLEAGSITTLNAFALGRSTAAGVGNGTLNIS
                     GGSLTLATPSKSAFLIGGVDGGHSARGGGNGVVNQTGGVVSVAHYMELGTRGGHGAYN
                     LDGGVLRIGGAGALRADTNGYDDTGDNVDDVTGTYEFNLGGGTLQVMGSNLTSAVAMT
                     LKSGTTSTIDTNGLGATFSGALSGMGALTKVGDGTLTFSGASTFSGGTLVSDGTLIVN
                     GSLANSTVSVGAGATLGGHGSVAGVNVLDDGRLELGGAPGTFTITGDLTLAGASRTLL
                     YLAAGSNDHLAVGGTLFAGGVLEVGLAGDFDLLEGASFDLFDGTLSRDFTNVLLPTLG
                     SGLEWSLVDFSSGVILVVTASAIPEPSTWTAILGGVVLAIAAWRRRYSQALETRRSE"
BASE COUNT          233 a          357 c          532 g          277 t
ORIGIN      
        1 ccagttatat cggatcaggc ctgggcaaca cgggcacggt agtgatgtcg ggcggcacgc
       61 tcaacgtcgg gcttgtcgcc acgccgggcg tgcgcgcgga tttcgaagtt ggttcgttca
      121 acggctcggg aacgatgacg cagacgggcg gcgacgttgt gctcaacacg acgggaggat
      181 tcttccacat cggcaaccaa ggcacgggtg tctataatct ggaggctggc agcatcacga
      241 cgctgaatgc gtttgcgcta gggcgctcga ccgccgcggg cgtgggcaac ggcacgttga
      301 acatctccgg tggctcgctg acgctcgcta cgccttcgaa atcagcgttc ttgattggtg
      361 gggtggatgg cgggcactca gcgaggggtg gcggcaatgg cgtggtcaat cagacgggtg
      421 gtgtcgtgag cgtcgctcac tatatggaac tcggcacgcg tggaggccac ggggcttata
      481 atcttgatgg cggcgtgttg cggattggcg gcgctggcgc gctgcgcgcc gacaccaatg
      541 gttacgatga cacaggtgat aatgtcgatg acgtcacggg cacctatgag tttaatctcg
      601 gcgggggcac gctccaggtg atgggcagca acttgacgtc ggcggtggcg atgacgctga
      661 agagcggcac gacttcgacg atcgatacca acgggctggg tgcgacgttt tcgggcgcgt
      721 tgtccgggat gggcgcgttg acgaaggtcg gcgatggcac gttgacgttc agtggagcga
      781 gcacgttttc cggcggaacg ttggtaagcg acggcacgct gatcgtgaac ggttcgttgg
      841 cgaactcgac ggtgagcgtc ggcgccggcg cgacgctcgg cgggcacggt tcggtggcgg
      901 gcgtgaacgt gctcgatgac ggacgactcg aactcggcgg cgcgccgggg actttcacga
      961 tcacgggcga cctcacgctc gcgggggcga gccgcacgct gttgtatcta gccgccggtt
     1021 cgaacgacca tctggcggtg ggtggcacgt tgttcgcagg cggtgtgctt gaggtcgggc
     1081 tggcggggga tttcgatctg ttggaaggtg cgagtttcga tctgttcgac gggaccttga
     1141 gcagggattt cacaaacgtg ctactgccga cgttggggag cgggcttgaa tggagcctcg
     1201 tggattttag cagtggcgtg atcttggtgg tgacggcttc tgcgattccc gaacccagca
     1261 cgtggacggc gattctgggc ggcgtggtgc tggcgatcgc ggcgtggcgg cggcggtatt
     1321 cgcaagcgtt ggagacgcga cggagcgagt gagtcgagtc gcgcgatatc ccgttaaaac
     1381 gtgcagggga ggcggcctc
//
LOCUS       DASPLT010000938         1527 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_276723, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000938 DASPLT010000000
VERSION     DASPLT010000938.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1527)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1527)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1527
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_276723"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..280)
                     /locus_tag="VEA63_09740"
     CDS             complement(<1..280)
                     /locus_tag="VEA63_09740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009508767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase"
                     /protein_id="HYD84326.1"
                     /translation="MPLPADLRAMLEAVRRIGRPRLVGGGVRDQLLGLEPKDFDIEVA
                     GADFESLHRALAPFGATDVVGRSFGVIKVRSAASGAEYDFSLPRRESKT"
     gene            complement(286..657)
                     /locus_tag="VEA63_09745"
     CDS             complement(286..657)
                     /locus_tag="VEA63_09745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009511722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HYD84327.1"
                     /translation="MLTAGILGATGVALGAFGAHGLERLLAERGTSATWETAVLYHLL
                     HAVALLALAPWLGTRAGVDQAIVRWTPRCWTIGTLLFSGSLYALALGGPRWFGPVTPL
                     GGAALIAGWVLLAVAAAKKPV"
     gene            933..1361
                     /gene="rplM"
                     /locus_tag="VEA63_09750"
     CDS             933..1361
                     /gene="rplM"
                     /locus_tag="VEA63_09750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HYD84328.1"
                     /translation="MKTFLAKKETVQPKWYLIDAAGVPLGRLAVKAANIIRGRHKASY
                     TPSVDNGDFVVVINAEKVALTGKKEEQNEYMFFSGFVGGESYRNMSQMRAKNPAFIIE
                     HAVKGMLPKNRIAAKMLTKLRVFAGEQHTHEANSPTKISV"
     gene            1372..>1527
                     /gene="rpsI"
                     /locus_tag="VEA63_09755"
     CDS             1372..>1527
                     /gene="rpsI"
                     /locus_tag="VEA63_09755"
                     /inference="COORDINATES: protein motif:HMM:NF012598.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HYD84329.1"
                     /translation="MSTVATNVFLGTGRRKTSTARVRITEGSGKLLCNGREFEDYFSH
                     ENFAKQAF"
BASE COUNT          314 a          479 c          480 g          254 t
ORIGIN      
        1 cggttttgga ttcgcgacgc ggcaggctga aatcgtattc ggcgccggag gcggcgctgc
       61 gcaccttgat cacgccgaaa ctgcgcccga cgacgtccgt cgcgccgaag ggcgcgagcg
      121 ctctatgcag cgattcgaaa tcggcaccgg cgacttcgat atcgaagtcc tttggctcga
      181 gcccgagcag ttgatcgcgc acgccgccgc cgacgagccg cgggcgtccg atccgccgga
      241 cggcttcgag catcgcgcgc agatcggcag gcaatggcat cgcgatcaaa cgggtttttt
      301 cgcggccgcg actgccagca agacccagcc ggcgatcaaa gcggcgcccc cgagcggagt
      361 gactggtccg aaccagcgcg gtccgccgag cgcgagcgca taaagcgagc cggaaaacaa
      421 caacgtgccg atcgtccaac aacgcggcgt ccagcgaacg atggcttggt cgacgcccgc
      481 gcgtgtcccc aaccacggtg cgagtgcgag cagcgcgacg gcatgcagca ggtggtaaag
      541 cacggcggtt tcccaagtcg cggacgtgcc acgttcagcc agcaaacgct ccagaccgtg
      601 tgcgccgaag gcgccgagtg cgacgccggt ggctcctaag attcctgcag tcagcaagag
      661 cgtggaggtg gaggaccgga cgcttgcttc gtgcggcgca cggggatcga ttggcgttcg
      721 ctgagtcata cgcgcaggtt tgcagatgcg cggtttgaaa caagctccgc tgtcgcgaag
      781 cgggcggtta agcgccaacg gctggcaggc acaaggcctg ccgccacgcc tgccgggggc
      841 gtcggcggga agatttcgcg tgccgtcgct tgcctctgga aaacaggggt tgacagcttt
      901 ccgcgaaacc ctagctcttg gcctcttttc ccatgaaaac cttcctcgcc aaaaaggaga
      961 cggtgcaacc caagtggtat ctcatcgatg ccgcgggcgt tccgctcggt cgtctcgctg
     1021 tgaaagccgc caacatcatc cgcggccggc acaaggcgtc ttacaccccg agcgtcgaca
     1081 acggcgactt tgttgtcgtg atcaacgccg agaaggtcgc gctgaccggc aagaaggaag
     1141 agcagaacga atacatgttt ttctcgggtt tcgtgggcgg tgaaagctac cgcaacatgt
     1201 cccagatgcg cgcgaagaat ccggccttca tcattgagca cgcggtcaaa ggcatgctcc
     1261 cgaagaaccg gatcgcggcc aagatgctga cgaagctccg cgtgtttgct ggcgaacagc
     1321 acacgcacga ggcgaacagc ccgaccaaaa tttccgtctg attagaagtc catgagcact
     1381 gtcgccacca acgttttcct cggcaccggt cgtcgcaaga cctccaccgc ccgcgtccgc
     1441 atcactgaag gcagcggcaa gctgctctgc aacgggcgcg agttcgaaga ttatttctcg
     1501 cacgaaaatt ttgccaagca ggcgttc
//
LOCUS       DASPLT010000939         1763 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_154477, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000939 DASPLT010000000
VERSION     DASPLT010000939.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1763)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1763)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1763
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_154477"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            478..618
                     /locus_tag="VEA63_09760"
     CDS             478..618
                     /locus_tag="VEA63_09760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 factor domain-containing protein"
                     /protein_id="HYD84330.1"
                     /translation="MLDHDTPEPLLTPEQEAELARRLANPGRKRVSHDEVAAHFAKKY
                     GR"
     gene            715..1551
                     /locus_tag="VEA63_09765"
     CDS             715..1551
                     /locus_tag="VEA63_09765"
                     /inference="COORDINATES: protein motif:HMM:NF016300.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HYD84331.1"
                     /translation="MFRLFVLVCVCLCGTGPALAQDAFDRPYWLDRSVIEARGSASVL
                     ASADNAAFSVTFQEVANDARSALFAASDRARLATAAMRARGGDAVYVRPEAEVTAIHA
                     EYRNRGGERLSSERADQVESYAASVRLAVQVTDMARVADIRAAAMAVGPEETSDVEYN
                     LLGNTQERLRVYRAAVQDASARARAAAEASGAALGRLLVLQDGQGPCLGRWQTGVSRP
                     QRQDFEAVSPVTTVGAEQIEVAVGSRALRLSAEDIARVQLPSDPPPIELTANVCAVYA
                     VG"
     gene            complement(1548..>1763)
                     /locus_tag="VEA63_09770"
     CDS             complement(1548..>1763)
                     /locus_tag="VEA63_09770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD84332.1"
                     /translation="NTKPPLIDGEARLVARSGDSDSHYARGDRIFHEKFGYGRIRQVE
                     GNKLTVDFEKAGEKRVIDSFVRPASAA"
BASE COUNT          317 a          520 c          577 g          349 t
ORIGIN      
        1 ccgagacggt aaacccgctg acgcgctgca cgtaaaggaa ctgcgcatac tcgatcacac
       61 ggtccagctt gtgactgaac cagtgccgga tgccgtccaa tcctgcttcc atcgaggtta
      121 tcgccgatag cgccattgtg cttctgcgct acgcttcatg caaaaccacg tccatcgaac
      181 aatcgtctca gccgaagttc cgtgcgcagc gtggttaagg cggcggcgct agaccggtag
      241 caacgcgcgc gcttaactcg tgcgcggcga atgtctctgc gattgcagcg tccttaacca
      301 caaggcccat gccgaagaat tcgtgcgttc agccccgata aagtgtcgcc gtgcactagg
      361 cgcaaccgat ctgggcgtct cgcttccgct acgcgctcgt ccgatcggtg cggtgatacg
      421 tacacccatc ccctcagtcg ctgcgccgga tcggatatag tcggggagat cgagttcatg
      481 ctggatcacg acacgcccga gcctctcttg acccccgagc aggaggccga gcttgcgcgg
      541 cgtctcgcca acccgggccg gaagcgcgtc tcgcacgatg aagtcgcggc gcacttcgcg
      601 aagaagtacg ggcgatgagg atcgatgtcc gcacatattg gtgagcacac cgaaacgtga
      661 atctgcgcat gacgcgacac tgatagttct ggattgaaca acgagggggg acctatgttc
      721 agattattcg tcttggtgtg tgtttgcttg tgcgggactg ggccagcgct tgcgcaagac
      781 gctttcgatc ggccgtattg gttggatcgt tcagtgatag aagcaagggg cagcgcatcg
      841 gtccttgcgt cggccgacaa tgcggcgttc tcggtaacgt ttcaggaagt ggcaaatgat
      901 gcgcgttcag ccttgtttgc agcatcggac agagcgcgtt tagcgacggc cgcaatgcgc
      961 gcacggggag gtgacgccgt gtatgtccgt ccagaggccg aggttacggc gatccacgcg
     1021 gagtatcgta accgcggagg cgagcggctc tctagcgagc gcgccgatca ggtcgaaagc
     1081 tacgccgcca gcgttagact cgccgtccag gtcaccgaca tggcgcgcgt ggcggatata
     1141 cgcgccgccg cgatggccgt tggccccgaa gagacgagtg atgtggagta caatttgctt
     1201 gggaacacgc aggagcgact tcgagtgtat cgcgccgcgg tgcaggatgc gtccgcccgc
     1261 gcgcgtgcgg ccgccgaggc aagcggtgca gcgctcggac gcttactcgt gctgcaggac
     1321 ggtcaaggtc catgcctcgg gcgatggcag acgggggtct cccgtccgca gcgtcaggac
     1381 tttgaggccg ttagcccggt cacaactgtc ggcgccgagc agatcgaggt tgcagttggc
     1441 tctcgcgctc tgcgactgag tgcggaagac atcgcccgcg tacaattgcc ttcggatccg
     1501 ccgccgattg agctgacagc caatgtctgt gcggtttatg cagttggcta agccgcgctc
     1561 gccgggcgca cgaagctgtc gatcacgcgc ttttcgccgg ctttctcgaa gtccacggtg
     1621 agcttgttgc cttcgacttg gcggatgcgg ccgtagccga acttctcgtg gaagatgcgg
     1681 tcgccgcgcg cataatggct gtcgctgtcg ccgctgcggg cgacgaggcg ggcttcgccg
     1741 tcgatcaggg gcggcttggt gtt
//
LOCUS       DASPLT010000940         1766 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_34745, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000940 DASPLT010000000
VERSION     DASPLT010000940.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1766)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1766)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1766
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_34745"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1147)
                     /locus_tag="VEA63_09775"
     CDS             complement(<1..1147)
                     /locus_tag="VEA63_09775"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HYD84333.1"
                     /translation="MRPILKLGAGLLFAVTKAAGEEGVSAGAAMDPLPVSAPSIGVGG
                     GLLIGALVLGGVIALFWKLQQRAIARERERLNQLRELELERLAAQARQAKEAADAANR
                     AKGEFLATMSHEIRTPLNGVIGSAELMLETPLNAQQREYMTTVRTSAEALLAIVNDIL
                     DFSKIEEGKVILDHTMFELRQPVVDVLKIAAARVGERELELVLDIAADVPGCIHGDPA
                     RLRQVLLNLVSNAVKFTTHGHVLVRIERMAERSDTERTWLKFSVTDTGIGIPAETRVR
                     LFEKFTQADASTTRRFGGTGLGLAISKRLVQLMGGEIGLESDPGHGSSFWFTLPMQVD
                     ELPATKGNPPAKRILVADDLPAAAEALAGLLRSLHVECVTATTAAEAM"
     gene            complement(1223..>1766)
                     /locus_tag="VEA63_09780"
     CDS             complement(1223..>1766)
                     /locus_tag="VEA63_09780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD84334.1"
                     /translation="TFLHRVHHMEFEAAGRFFGLALVATGLAATLIGGLAGTAWQRRS
                     PGGYSSILVMSASITAPVAFAAFVLDQGTAARVALVAAMFFMFLPTGPINTLILETVP
                     AAQRARAMAGSIFAIHAFGDLWSPWIVGRLSDRWGSLQSAVMILPGALVVAAFFWGWL
                     AVHQRRGARQQKTVVSETIG"
BASE COUNT          341 a          706 c          429 g          290 t
ORIGIN      
        1 ccattgcttc cgccgccgtc gtcgccgtca cgcactcgac atgcagcgag cgcaacaatc
       61 ccgccagcgc ctccgccgcc gccggcagat cgtccgcgac gaggatccgc ttcgccggag
      121 gattgccctt cgtcgccggc aactcgtcca cctgcatcgg cagcgtgaac caaaacgacg
      181 acccgtggcc cggatcgctt tccaaaccga tctcgccccc catcagctgc accagccgct
      241 tgctgatcgc cagccccagc cccgttccgc cgaatcgccg cgtcgtcgac gcatcggcct
      301 gcgtgaattt ctcgaacagc cgcacccgcg tctccgccgg aatgccgatg cccgtgtccg
      361 tcaccgaaaa tttcaaccac gtccgctccg tgtcgcttcg ctccgccatc cgctcgatcc
      421 gcaccagcac gtgcccgtgc gtcgtgaact tcaccgcgtt cgacacgaga ttgagcaaca
      481 cctgccgcaa ccgcgcggga tcgccgtgaa tgcacccggg cacgtctgcg gcgatgtcca
      541 gcaccagctc cagctcgcgc tcaccgacgc gcgccgccgc gatcttcaac acgtcgacca
      601 ccggctgccg cagctcgaac atcgtatgat cgaggatcac tttgccttcc tcgatcttcg
      661 aaaaatccag gatgtcgttc acgatcgcca gcaacgcctc ggccgacgtc cgcaccgtcg
      721 tcatatattc gcgctgctgc gcgttcaacg gcgtctcgag catcaactcc gccgacccga
      781 tcacgccgtt gagcggcgtg cggatctcgt ggctcatcgt cgcaagaaac tcgcctttcg
      841 cgcgattcgc cgcgtccgcc gcctccttcg cttgccgcgc ctgcgccgcc agccgctcca
      901 gctccagttc gcgcaactgg ttcaaccgct cccgctcccg cgcgatcgcc cgctgctgca
      961 gcttccaaaa caacgcgatc acaccgccca gcaccaaagc cccgatcaac aacccacctc
     1021 cgacgccgat gcttggcgcc gacacgggaa gcggatccat cgccgcaccg gcgctcacgc
     1081 cctcttcgcc ggcggccttg gttactgcaa agagcagtcc ggcaccgagt tttaaaatcg
     1141 gcctcaactc tacgagtcct tggggtccat cgctctgcac ggtgggctgt ctaccctaga
     1201 ccaaaacaat tgcaagggat gttcacccga tcgtctcact cacaaccgtt ttctgctgcc
     1261 tcgcgccccg gcgctggtgc accgccagcc acccccaaaa aaacgccgcg accaccagcg
     1321 cgcccggcag gatcatcacc gccgattgca agcttcccca gcgatccgac aaccgtccga
     1381 caatccatgg cgaccacaaa tcgccaaacg cgtgaatcgc gaaaatcgat cccgccatcg
     1441 ctcgcgcccg ctgcgccgcc ggcaccgttt ccaaaatcaa cgtgttgatc ggtcccgtcg
     1501 gcaaaaacat gaaaaacatc gccgccacta acgccacccg cgccgccgtc ccctgatcca
     1561 gcacaaacgc cgcgaacgcc actggcgccg tgatgctcgc gctcatcact aaaatggagc
     1621 tgtagccacc cggacttcgc cgctgccacg cggtgcccgc caatccgcca atcaatgtcg
     1681 ccgccaatcc cgtcgcgacc agtgccaacc caaaaaaacg ccccgccgcc tcgaattcca
     1741 tgtgatgcac ccgatgcaaa aacgtc
//
LOCUS       DASPLT010000941         2194 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_154540, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000941 DASPLT010000000
VERSION     DASPLT010000941.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 2194)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2194)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2194
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_154540"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            308..2110
                     /locus_tag="VEA63_09785"
     CDS             308..2110
                     /locus_tag="VEA63_09785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HYD84335.1"
                     /translation="MALVSLLDVSLSFGGAPLLDRVNLQIDRGERVCLVGRNGAGKST
                     LMKVIAGELQPDEGQVFRQPGSGFSMLRQEVPVGVEGTVRTVVEGEGGSYEEHNDWER
                     HDRVERLLEQMGLPTDAEFASLSAGQKRRVLLARGLVEAPQLLLLDEPTNHLDLESIE
                     WLENFLLEWGGALLFVTHDRAFLRKLATRIVEVDRGKLVGWSCDYDTFLVRKQAVLEA
                     EEVQRAQFDKKLAQEEEWIRRGVKAQRSRAQNRIHALEKMRAERAARRERTGTAKLTA
                     QEADRSGFKVIACENAGFSYDGERWIVRDLTTRIERGDKIGIVGPNGAGKTTLLRLLL
                     GQLAPTTGTIEQGTRLEIVYFDQLRAQLDDTMRVQDAVADGNATVTINGRTRHVISYL
                     EDFLFEPARARTPIKALSGGERNRLLLARLFTKPCNVLVLDEPTNDLDAETLELLEDL
                     LVEFGGTLLLVSHDRAFLNEVCTSLLVFEGEGKVTEYVGGYDDWQKEKAARAAAAEKQ
                     RELEKEQAKRAAANAVVEKKARKLSNKERGELEALPKRIESLETEQAALTGTLADPAF
                     FKKGGAEVAKATTRLHEIEAELAAAFARWGELEG"
BASE COUNT          421 a          568 c          832 g          373 t
ORIGIN      
        1 cgcgggtttg cggagtggag gggcggggca tcggacgggg tgcgccgatt cacgcgcaac
       61 gtggcggcac ggcaaatctt tttgcggtaa aggcagcgtt gaacgtcccg ctgctcacgc
      121 agcgagccac atcgagccgc ctgctactca cacagcgagc cacatcgaac cttccccgca
      181 tcgagactcc tgctgatcga ctgcggaggt ccggacgtgc gcaggaagcg gcggcatttc
      241 gcgacgtgaa agacggtgcg atggggtctt gcgcagggcc gtgcgggtgg cagggtgatc
      301 ggttcctatg gcgcttgtga gtctactcga tgtcagtctg agcttcggcg gtgcgccgtt
      361 gctcgaccgc gtgaaccttc aaatcgatcg cggtgaacgc gtgtgtctcg tcggccgcaa
      421 tggcgcggga aaatccacgc tgatgaaggt gatcgccggt gagttgcagc ccgacgaggg
      481 gcaggtgttt cgccaacccg gctcgggttt ctcgatgtta cggcaggaag tgccggtggg
      541 ggtcgaagga acggtgcgca cggtcgtcga gggcgagggc ggcagctacg aagagcacaa
      601 cgattgggag cgacatgacc gcgtggagcg actgctggaa cagatgggac tgccgactga
      661 cgcggaattt gcgtcgcttt cggcggggca gaagcggcgg gtgttgctcg cgcgaggttt
      721 ggtcgaggcg ccgcaattgc tgctgctcga cgagccgacg aaccatctcg atctcgagtc
      781 gatcgagtgg ctggagaatt tcttgttgga gtggggcggt gcgctgttgt tcgtgacgca
      841 cgatcgcgcg tttttgcgga aactggcgac gcggattgtg gaagttgacc gcggcaagct
      901 cgtcggctgg tcgtgcgact acgatacgtt tctcgtgcgc aagcaggcgg tgctcgaggc
      961 ggaggaggtg cagcgggcgc agttcgacaa gaaactcgcg caggaggagg agtggattcg
     1021 ccgcggtgtg aaggcgcagc gttcgcgcgc gcagaaccgt attcacgcat tggaaaagat
     1081 gcgtgcggaa cgcgcggcgc ggcgggagcg aacgggcacg gcgaagctta cggcgcagga
     1141 ggcggatcgc agcgggttca aggtgatcgc atgcgagaac gcgggcttta gctatgacgg
     1201 cgagcggtgg atcgtgcgcg atctcacgac gcgcatcgaa cgcggcgaca agatcggcat
     1261 cgtcgggccg aacggcgcgg gaaaaacgac gctgttgcgg ctcttactcg ggcagctcgc
     1321 gccgacgacc ggcacgatcg agcagggcac gcgattggaa atcgtgtatt tcgaccagct
     1381 gcgcgcgcag ctcgacgaca cgatgcgcgt gcaggatgcg gtggcggacg gtaatgcgac
     1441 cgtgacaatc aacggccgga cgcggcacgt gatttcatat ctggaggatt tcctgttcga
     1501 gccggcgcgc gcgcgcacgc cgatcaaggc cttgtcgggc ggagagagaa accggttgtt
     1561 gctcgcgcga ttgttcacaa agccgtgcaa cgtgctcgtg ctcgacgagc cgacgaacga
     1621 tctggacgcg gagacgctcg agttgctgga ggatttgctc gtggaattcg gtggcacgct
     1681 gctgctggtg agccacgatc gcgccttcct caacgaagtg tgcacgagcc tgctggtgtt
     1741 cgaaggcgaa ggaaaggtga cggagtatgt cggcggctac gacgactggc agaaggaaaa
     1801 agcggcgcgc gccgcggcag cggaaaagca gcgcgagctg gagaaggagc aggcgaagcg
     1861 cgcggcggcc aacgccgtcg tggagaagaa ggcgcgcaaa ctgtcgaaca aggaacgcgg
     1921 cgagctcgag gcgctgccaa agcgtatcga aagcttggag acggagcagg cggcgttgac
     1981 cgggacgctg gccgatccgg cgtttttcaa gaaaggcggc gcggaagtgg cgaaggccac
     2041 gacgcgcttg cacgagatcg aagcggagct ggcggcggcg tttgcgcggt ggggcgagct
     2101 ggaggggtga cgcgcggcgg attggaggaa cgcggttggg cgaatttcga cgacggaagt
     2161 cggagttttt taaccgctaa tttgcgctga tttt
//
LOCUS       DASPLT010000942         1775 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_215274, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000942 DASPLT010000000
VERSION     DASPLT010000942.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1775)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1775)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1775
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_215274"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            8..139
                     /locus_tag="VEA63_09790"
     CDS             8..139
                     /locus_tag="VEA63_09790"
                     /inference="COORDINATES: protein motif:HMM:NF013706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HYD84336.1"
                     /translation="MITTGTPPGVGLGMKPPRYLSAGDVVTLEIDSLGRQRSVCRNA"
     gene            373..1374
                     /locus_tag="VEA63_09795"
     CDS             373..1374
                     /locus_tag="VEA63_09795"
                     /inference="COORDINATES: protein motif:HMM:NF015132.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HYD84337.1"
                     /translation="MPPAPTPTVAKFRDWCLLLLCNFIWASQFALVKLVQDEVGPLFT
                     TAFPMLLATVLLAPVVWWQRRRPVQLDVAYATSESTAGVRVPARVRGPDIAGFVTIGL
                     CGQVVAQLFATWGSQQSLASNAALISLALPVATALMAFLLLRERMTRLRWIGFALAIA
                     GVLVSSANDLRTADLRSRSYLWGNSMVFLGVLGSAFYNVYSKKLLARFTPLEVLLASY
                     IAACLLLVPVALIVEPDGLARLALMSSRGWTGLLLLAVFQYCASMILFLTVLTRLDAT
                     QAALSNYLIPMFGVLIAWLLLGERLQPVAIAGGVLVLASTLLVTVWEERMNARRNAS"
     gene            1371..>1775
                     /locus_tag="VEA63_09800"
     CDS             1371..>1775
                     /locus_tag="VEA63_09800"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HYD84338.1"
                     /translation="MSAEPTPAKSFAGKNVLVTGAARGLGLEIARQFARSGASLHLFD
                     LHADLLTQAGRELATHGVRVATWRVDVRQRAEVEQAVAAVEAQAPIDILINNAGIAHE
                     TAFLKIDEREWREILDVNLTGLFFVAQAVTRAM"
BASE COUNT          256 a          647 c          513 g          359 t
ORIGIN      
        1 cggcgacgtg atcacgaccg gcacgccgcc cggtgtcggc ctcggcatga aaccgccccg
       61 ttacttgagc gccggcgacg tcgtcacgct cgagatcgac agcctcggtc ggcaacgctc
      121 cgtctgccgc aacgcctgag cgcctccgcg gggagcctgc tttcaggcga ttggcgcgcc
      181 gccaatcgct tcgcgtcgcg cgtcttttcg cgctccttcg ccatccctaa ccgccgcgcg
      241 cgaaaacggg ttcaccgcgt ccccccttct cttccttccc gcttcctccg tcatcctgag
      301 cgaatgcaaa ggatctccgt tttcttcgcg ctccccattc cctgtcgcgg ttagcggcgc
      361 cgcgcacacc ccatgccccc cgcacccact cccaccgtcg ccaagttccg cgactggtgc
      421 ctgctgctcc tctgcaattt catttgggca agccagttcg ccctcgtgaa actggtgcaa
      481 gacgaggtcg gcccgctgtt caccaccgcg ttcccgatgc tgctcgccac cgtcctgctc
      541 gcgcctgtcg tctggtggca gcgccgccgc cccgtgcagt tggatgtcgc atatgcgaca
      601 tcggaaagca cggcgggcgt tcgcgtgccg gcccgcgttc gcggccccga catcgccggc
      661 tttgtcacca tcggactttg cggccaggtc gtcgcgcaac tgttcgccac ctggggctct
      721 caacaatcgc tcgcctcgaa cgctgcgctg atctcgctcg cgctgcccgt cgcgacggcg
      781 ttgatggcct ttctccttct gcgcgagcgg atgacgcgcc tgcgctggat cggcttcgcg
      841 ctcgcgatcg ccggcgtgct tgtgagttcg gcgaacgatt tgcgcacggc cgatctccgc
      901 agccgcagtt atctatgggg aaattccatg gtgttcctcg gcgtgctcgg cagcgccttc
      961 tataacgtct acagcaaaaa attgctcgcg cgcttcacgc cgcttgaagt gctgctcgcc
     1021 agctatatcg ccgcctgcct cctgctcgtc cctgtcgctt tgatcgtgga gcccgacggc
     1081 ctcgcgcggc ttgcactcat gagttcgcgc ggttggaccg gtttgctgtt gctcgcagtc
     1141 ttccaatatt gcgcgtcgat gatccttttt ctcaccgtgc tgacgcggct cgacgccacg
     1201 caggccgcgc tctcgaacta cctcatcccg atgttcggcg ttctcatcgc gtggctcctg
     1261 ctcggcgaac gactgcagcc cgtggccatc gcgggcggcg tgctggtgct cgccagcacc
     1321 ttgctggtca ccgtttggga agagcggatg aacgcgcgga gaaacgcttc atgagcgccg
     1381 agcccacacc ggctaaaagc ttcgccggaa aaaacgtcct cgtaaccggc gccgctcgcg
     1441 gactcggcct cgagatcgcc cgccagtttg cccgcagcgg cgcgagtctg cacctgttcg
     1501 atcttcacgc cgacctgttg acccaagccg ggcgcgaact cgccacacac ggcgtccgcg
     1561 ttgcgacgtg gcgcgtcgac gttcgccagc gcgccgaggt cgagcaagcc gttgccgccg
     1621 tcgaagccca agcgcccatc gacattttga tcaacaacgc cggcatcgcc cacgagaccg
     1681 cgttcctgaa aatcgacgag cgcgaatggc gcgaaatcct cgacgtgaat ctcaccggcc
     1741 tgttcttcgt tgcgcaagcg gtgacgcgtg cgatg
//
LOCUS       DASPLT010000943         1743 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_196469, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000943 DASPLT010000000
VERSION     DASPLT010000943.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1743)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1743)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1743
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_196469"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1708)
                     /locus_tag="VEA63_09805"
     CDS             complement(<1..1708)
                     /locus_tag="VEA63_09805"
                     /inference="COORDINATES: protein motif:HMM:NF017197.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF748 domain-containing protein"
                     /protein_id="HYD84339.1"
                     /translation="MKYRKRSRWRIIVVLVALYAALGFFALPPLVKWQLEKRLTETLA
                     RPVSVGQVRTNPFALSIAVNRVVVRVPDGSDTLVQWNRFFVNFDPLASITREWVLGDV
                     AVDGLSAAIEVRPDGSLNISDLIEKFRPPESRDETPRPAEKPSRPWRIQRLNITDARV
                     EFADASQAEPFHTTLGPVNLTVLDFRTAGLRSAPGRFEAVSDSGEKMTWIGSFTAAPL
                     TSTGELRVENVLLPKYAPYLAERLRATITSGRLGLSVNYDIDLSPGNRRFAITDGTTQ
                     LSEFHLKENDGGEPLVELGSFQVAGIVADGFAPRVAIEAIRLDGGTLTVRRDAEGVLN
                     WLGLLPTDDGAAASSTQPPKQPLDLNVKEISLRDFAITARDLSMPQPVEASLTRINGS
                     LKNATLAAGAAMPIDLSFDWAPRGAVRVNGAVMREPLMADLDFEIAWLALPPLSPYLE
                     ERLNARMARGAASMNGHARVELKEAAPPDVTFSGGGRVEDFSLVASEGGQELASLSEL
                     AFNDVKLTTSPRTVLSIAEVSVGSPQAHVTVNEEGELNFAQLVRAPVEGQEQSADAAG
                     EGT"
BASE COUNT          320 a          652 c          475 g          296 t
ORIGIN      
        1 tcgtcccctc gcccgccgcg tccgcgctct gctcttggcc ctcgaccggt gcccgcacga
       61 gctgcgcaaa gttcaactcg ccttcctcgt tcacggtcac gtgcgcctgc ggcgagccca
      121 cgctcacttc cgcgatcgac agcaccgtcc ggggcgacgt cgtcaacttc acatcgttga
      181 acgccagctc ggaaagactc gcgagttcct gcccgccttc cgacgccacc aagctaaaat
      241 cctccacgcg cccgccgccg gaaaacgtca catcgggcgg cgccgcttct ttcaactcca
      301 cgcgcgcgtg cccgttcatc gatgccgcgc cgcgcgccat gcgcgcattg agcctctcct
      361 ccagatatgg gctcaacggc ggcaaagcca gccacgcgat ctcgaaatcc aaatccgcca
      421 tcagcggctc ccgcatcaca gccccgttca cgcgcaccgc gccgcgcggc gcccagtcga
      481 acgacaaatc gatcggcatc gccgcgcccg ccgccagcgt cgcgtttttc agcgagccat
      541 tgatgcgcgt caggctcgcc tcgaccggct gcggcatcga caaatcgcgt gccgtaatcg
      601 cgaagtctcg caacgaaatt tccttcacgt tcaaatcgag cggctgcttc ggaggctgcg
      661 tcgacgacgc cgccgcgccg tcgtccgtcg gcagcaaacc gagccagttc agcacgccct
      721 cggcgtcccg acgcaccgtg agcgtgccgc catcgagccg gatcgcttcg atggcgacac
      781 gcggcgcaaa cccgtccgcg acgattcccg ccacttgaaa cgaacccagc tccacgagcg
      841 gctcgccgcc atcgttttcc ttcaggtgaa attcgctgag ttgcgtcgtc ccatccgtga
      901 tcgcaaaccg gcgattgccc ggcgagagat cgatatcgta gttcacactc aagccgagcc
      961 ggcccgacgt gatcgttgcg cgcaaccgct ccgcgagata cggcgcgtat ttcggcagca
     1021 gcacattctc gacgcgcaac tcgcccgtcg atgtcaacgg cgccgccgtg aacgaaccga
     1081 tccatgtcat tttctccccc gaatcgctca ccgcttcgaa ccgcccgggc gcgctgcgca
     1141 gtcccgccgt gcggaaatcc agcaccgtga gattcaccgg tccaagcgtg gtgtgaaacg
     1201 gctccgcttg cgacgcgtcg gcgaactcca cgcgcgcatc cgtgatattc aaacgctgga
     1261 tacgccacgg ccgcgacggc ttctccgccg gccgcggcgt ttcgtcgcgc gactccggcg
     1321 gtcgaaattt ttcgatcaga tcggaaatgt tgagcgaacc gtccggccgc acttcgatcg
     1381 ccgcgctcaa tccatcgacc gccacgtcac cgagcaccca ctcgcgcgtg atcgacgcga
     1441 gcggatcgaa gttcacgaaa aaccggttcc actgcaccag cgtgtccgaa ccgtccggca
     1501 cgcgcacgac cacgcggttc accgcgatcg acaacgcaaa cggatttgtc cgcacctgcc
     1561 ccacgctcac cgggcgcgcg agcgtttcgg tcagccgttt ttcgagctgc catttcacca
     1621 gcggcggcaa cgcgaaaaac cccagcgccg cataaagtgc gaccagcacc acaatgatcc
     1681 gccagcggct gcgttttcga tatttcatgg cttcgggggt gaagcgtcat cgacaagcga
     1741 ttc
//
LOCUS       DASPLT010000944         1517 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_92574, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000944 DASPLT010000000
VERSION     DASPLT010000944.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1517)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1517)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1517
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_92574"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..251)
                     /locus_tag="VEA63_09810"
     CDS             complement(<1..251)
                     /locus_tag="VEA63_09810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD84340.1"
                     /translation="MKKLFPWIVLAIGVLYAASSFRALRNPSEHTAFDIVGFGQLPVV
                     TNGRVKPLDTVARTSILLFQGRQRVAIPELSPPASGEPER"
     gene            complement(248..1417)
                     /locus_tag="VEA63_09815"
     CDS             complement(248..1417)
                     /locus_tag="VEA63_09815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007358899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein ResB"
                     /protein_id="HYD84341.1"
                     /translation="MNDITRQFRDFFVSLKLTVVLLVLSVILIFAATLDQVNLGIWAV
                     QEKYFRSLFVLWRVGDVPVPVFPGGYLIGGFLLVNLIAAHVYRFKLTWKKSGIFLTHL
                     GLILLLVGELLSGLWQEDYQMRLVEGETKNYSESFRNNEFVIVETTDPKFDDVVAIPE
                     DILADRSELQHPKLPFRIVVKEYFPNAVLQLRSNMPREVPAAATQGVGPKLVVTPVPI
                     TYKQDERNAPAAVIELVGTEGSLGTWAVSTHLPASQTVSYAGRTWRLGLRFERAYKPF
                     ALTLLKVTHDVYLGTDIPKNFSSRVRLQSADGSDDREVLIYMNNPLRYAGLTFYQYQM
                     DSANATSVLQVVRNPSWLIPYISCAMMTLGLLIQFGIHLFSFATRQRRSVPVTTP"
BASE COUNT          309 a          392 c          542 g          274 t
ORIGIN      
        1 cgctccggtt cgccggaggc cggcggcgac aactcgggaa tggcgacgcg ctggcgacct
       61 tggaagagca ggatcgaagt gcgggcgacg gtatcgagcg gtttgacgcg gccgttcgtg
      121 acgacgggaa gctggccgaa accgacgatg tcgaacgcgg tgtgttccga tggattgcgg
      181 agtgcgcgga aggagctggc ggcgtaaaga acgccgatcg cgagaacgat ccaggggaaa
      241 agttttttca tggcgtggtg accggaacgc tgcggcgctg ccgggtggcg aaactgaaaa
      301 ggtgaatgcc gaactggatg aggagcccga gcgtcatcat cgcgcaggag atataaggaa
      361 tcagccagct cgggttgcgc acgacttgga ggacgctcgt cgcgttggcg ctgtccattt
      421 ggtattggta gaacgtcaac ccggcgtaac gcagcggatt gttcatgtag atgagcacct
      481 cgcggtcgtc gctgccgtcg gcgctttgga ggcgcacgcg gctggagaaa tttttcggaa
      541 tgtcggtgcc gagataaacg tcgtgcgtga ccttcagcag cgtgagcgcg aagggcttgt
      601 aagcgcgctc aaaacgaagc ccgagccgcc acgtgcggcc ggcatagctc acggtctgcg
      661 acgcgggcag gtgcgtcgat accgcccagg tgccgagcga gccttcggtg ccgacgagct
      721 cgatcacggc ggcgggcgcg ttgcgttcgt cctgtttgta ggtgatcggc acgggcgtga
      781 cgacgagctt ggggccgacg ccttgggtgg ccgcggcggg gacttcgcgc ggcatgttgg
      841 agcggagctg cagaacggcg ttgggaaaat attctttcac cacgatgcgg aacggcagct
      901 tcgggtgctg aagctcggat cgatcggcga ggatgtcctc gggaatcgcg acgacgtcgt
      961 cgaacttcgg gtcggtggtc tcgacgataa cgaactcgtt gttccgaaaa ctttcggagt
     1021 agttctttgt ttcgccttcg acgaggcgca tttgataatc ctcctgccag agcccgctga
     1081 gcagttcgcc gacgaggagg agaatcaaac cgaggtgcgt gagaaagatg ccggattttt
     1141 tccaagtcag cttgaagcgg taaacgtgcg ccgcgatgag gttcacgagc aggaacccgc
     1201 cgatgagata accgccggga aacaccggca cgggcacgtc gccgacgcgc cagagcacga
     1261 agagcgagcg gaaatatttt tcctgcacgg cccagatgcc gaggttcact tgatcgagcg
     1321 tggcggcgaa gatcaggatc accgacagca cgagcagcac gacggtgagt ttgagcgaca
     1381 cgaagaagtc gcggaattgg cgagtaatgt cgttcatcgg ccggcgggcg gttggatggt
     1441 ttggagaaac gcgcggaagg tttcgcgctc cgccgccacg agcgcgtcgg gtcccatgag
     1501 tttgaaaaac caggtgg
//
LOCUS       DASPLT010000945         1527 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_50972, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000945 DASPLT010000000
VERSION     DASPLT010000945.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1527)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1527)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1527
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_50972"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..577)
                     /locus_tag="VEA63_09820"
     CDS             complement(<1..577)
                     /locus_tag="VEA63_09820"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Plug domain-containing protein"
                     /protein_id="HYD84342.1"
                     /translation="MTKLGHTHGKTARVTFAAGLLTCLALQGQAFAQATVSETTPDDG
                     MIVLSPFEVRSDLDTGYLATSAQSGTRLRTDLKDIASSISVITKDFMNDIGAKDLEGL
                     LVYTLGTEVNGVGGNFSDAGTIDNPNGAETDYDAAFASATPGTRVRGLTSADLSRDFF
                     VSRLPVDAYNIERLEISRGPNAMLFGLGSPSG"
     gene            1037..>1527
                     /locus_tag="VEA63_09825"
     CDS             1037..>1527
                     /locus_tag="VEA63_09825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007359936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HYD84343.1"
                     /translation="MGSFPIAQTVTSQVMKISYRFPRSFVWGVAASAPQIEGAAAEGG
                     KGESIWDRFARQPGKIDERHTPAVACDHYHRFKNDFALMRRLGVKHYRLSLAWPRLFP
                     DGGRTVNARGVDFYRRLFDAMASNDITPWVTFYHWDLPQALEENGGWRQRATVDAFAH
                     YCET"
BASE COUNT          292 a          469 c          464 g          302 t
ORIGIN      
        1 tacccgaggg gctgccgaga ccgaacaaca tggcgttcgg accgcggctg atttccaagc
       61 gctcgatatt ataagcgtca accggcagcc ggctgacgaa gaagtcgcgg gaaagatccg
      121 ccgaggtcag cccgcggacg cgcgtgcccg gtgtcgccga ggcaaacgcc gcgtcgtagt
      181 ccgtctccgc cccgttggga ttgtcgatcg tgcccgcgtc ggaaaagtta ccgcccacgc
      241 cgttgacttc ggtgcccagc gtgtagacga gaagtccttc gaggtccttc gccccgatgt
      301 cgttcatgaa gtccttcgta atgaccgaaa ttgaggaggc gatatccttc aaatccgtgc
      361 gcagacgggt gccggattgc gcgctggtcg ccaagtaacc ggtgtcgaga tcgctccgca
      421 cttcgaacgg cgagagaacg atcatcccgt cgtccggcgt cgtttccgag acggtcgctt
      481 gagcaaaggc ctgcccctga agggccaggc aggtgaggag gccggcagcg aaagtgactc
      541 tcgctgtctt tccgtgggtg tggccgagtt tagtcatagg tgtttgcgag ctacttggtg
      601 tgttttttcg ggctaaaggg actccgcatt tgacaggtgt cacgcgggcc ggggggaaaa
      661 aggacggtgc agatggaacg acggcgcgca tgaaagaggg gtgagcagca atcgttccac
      721 gaaccaatgc gcgttggatt gcacgtgtca acgatggaac ttcgtcgaaa tgtggaaact
      781 ttcgccactg tccgtcaggc ctggcggctt ggtggcgtcg agttcggaaa ttgaccgcca
      841 gtctgacgta ttttcccctg ccgcagctgt gcataatttc gctgctcggc gcgccgaaat
      901 tggcagcggc agcggaccct ctcgacgaat cgccaccacc gagcgctaaa atgcgccgcc
      961 ttggcccggt ttccaccggc tttgccttgc caaccgccgt caccggaatc caaatccgcc
     1021 gctttttcac atgcgcatgg gttcctttcc catcgctcaa accgttacct cgcaggtcat
     1081 gaagatctcc tatcgttttc cgcgcagctt tgtctggggc gttgccgcct ccgcacctca
     1141 aatcgaaggc gccgctgccg aaggcggcaa aggcgaatcg atctgggatc gattcgcgcg
     1201 ccagcccggc aaaatcgacg aaaggcacac ccccgccgtc gcctgcgacc actatcatcg
     1261 cttcaaaaac gacttcgccc tcatgcggcg gctcggcgtg aaacactacc ggctctcgct
     1321 ggcgtggccg cgtctctttc cggacggcgg acgcacggtc aacgcccgcg gcgtcgattt
     1381 ttatcggcgc ttgttcgacg cgatggcgag caacgacatc acgccgtggg tgacgttcta
     1441 ccactgggat cttccacaag cgctcgagga aaacggcggc tggcgacagc gcgcgaccgt
     1501 cgatgcgttc gcacactact gcgaaac
//
LOCUS       DASPLT010000946         2460 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_236195, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000946 DASPLT010000000
VERSION     DASPLT010000946.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 2460)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2460)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2460
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_236195"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2091
                     /locus_tag="VEA63_09830"
     CDS             <1..2091
                     /locus_tag="VEA63_09830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD84344.1"
                     /translation="GADKRTFNLGGPDNYNNQQQWNLEVVGTQQLLRESDFLPQADLL
                     IGYNRTGYYVQTRDANGGIQQVSPGTPGFNLSQTIVLTSIDNGLGSGVTPSNGNLQYG
                     TFDNEVVRYAWNKRDVETYRDQIRAELNLQKSLFAGRWFQLQDQVLVGYSELYNEVQR
                     NEWETVPDLYSFKGPLDLDPIRFATQGDGTTAPGLFQDVRDNISRGWNRAFYLNNYLK
                     FGKLWGLEDRVILMTGIRRDISDNWATTTEITGPYATPPSVATTTTRVARQTKVDSKQ
                     LGVMFRLTKSLSVFALKADGFQPNFGELRESLTGAPVGADTAESKEIGIKFDFLDGKI
                     SGTISSYRIKKKAWLGQGFSTPAPLGDPRFDPSKPIIYNLGDANGTGFYNPFPGQFTP
                     NGQTYAPNAAQQAAWIAAANAGAITLTSPINGKRETESSIYLNASTPEGAAWMDAFFD
                     NARTSGWAGWAYYGNDINDPGINNATLDDGAFQNTDTPNAIPSTSESEGWDGSILFTP
                     NDQWQFVFSGAFGTKVQLIDKGKWIKYPYPQDRWATWYFPNGGFGLKGQTLQEAYTDP
                     TDTSTRTNSGVFPGDDTPENRFSIFANYKFKDALKGWIVGAGGDWQSKRAYFSGVTHG
                     SGQVQTDVDGELIVLYMPEQWTLNAFVRKEWRRGRYDHSVQLNVDNVINDRDLYGQIY
                     MAPISARITYQVGF"
     gene            2268..>2460
                     /locus_tag="VEA63_09835"
     CDS             2268..>2460
                     /locus_tag="VEA63_09835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HYD84345.1"
                     /translation="MTTPSSERLSFKEKLAYGLGDTASNFYFQAFNLFLVYYYTDIFG
                     LPSAAVGTLVLIVPIAVAAL"
BASE COUNT          501 a          814 c          700 g          445 t
ORIGIN      
        1 ggcgccgata aacgcacgtt caacctcggc ggccccgaca actacaacaa ccagcagcag
       61 tggaatctcg aggtcgtcgg cacccagcag ctcctccgcg aaagcgattt cctgccgcaa
      121 gccgacttgt tgatcggcta caaccgcacc ggctactacg tgcagacgcg cgacgcgaac
      181 ggcggcatcc agcaggtttc gccaggcacg ccgggtttca atctttcgca gacgatcgtg
      241 ctcacatcga tcgacaacgg tctcgggagc ggtgtcacgc cgtccaacgg caatctccaa
      301 tacggcacgt tcgacaacga agtcgtgcgc tatgcgtgga acaagcgcga cgtggaaacc
      361 taccgcgacc agatccgcgc cgagctcaat ctccagaagt cgctgttcgc cggtcgctgg
      421 ttccagcttc aggatcaggt gctcgtcggc tactcggaat tatataacga agttcagcgc
      481 aacgaatggg agaccgttcc cgatctctat agctttaaag gtccgctcga tctcgacccc
      541 atccgcttcg ccacccaggg cgacggcacg actgctcccg gcctgttcca ggatgttcgc
      601 gacaacatca gccgcggctg gaatcgcgcg ttctatctga acaattatct caagttcgga
      661 aaactctggg gcctcgagga ccgcgtcatc ctgatgaccg gcatccgccg cgacatcagc
      721 gacaactggg caacgaccac ggagatcacc ggcccctacg ccacgccgcc gagtgtcgcc
      781 acgacgacga cgcgcgtcgc gcggcagacc aaggtcgatt cgaagcagct cggcgtcatg
      841 ttccgtctta cgaaatcgtt gtccgtcttc gcgctgaaag cggacggatt ccaaccgaac
      901 ttcggtgagc ttcgcgaatc gctcacgggc gcgcccgtcg gggccgacac cgccgagagc
      961 aaggaaatcg gcatcaagtt cgattttctc gacggcaaga tttcgggcac gatcagcagc
     1021 tatcgcatca agaaaaaggc ctggctcggg cagggcttct cgacgccggc gccgctcggc
     1081 gatccgcgtt tcgatccgtc gaagccgatt atctataacc tcggcgacgc gaacggcacc
     1141 ggtttctaca atccgtttcc cggccagttc acgcccaacg gccagaccta cgcgccgaat
     1201 gctgcgcagc aggccgcatg gatcgccgcc gccaacgctg gcgcgatcac gctgacgtcg
     1261 ccgatcaacg gcaagcgtga gaccgaatcc agcatttatc tcaacgccag cacgcccgaa
     1321 ggcgcggcgt ggatggacgc gttcttcgac aacgcgcgca cctccggctg ggcgggctgg
     1381 gcttactacg gcaacgacat caacgacccg ggcatcaaca acgccacgct cgacgacggc
     1441 gcgtttcaga acaccgacac gccgaacgcc attccgtcga cgtccgagtc ggaaggctgg
     1501 gacggctcga ttcttttcac gccgaacgac cagtggcagt tcgtgttctc cggcgcgttc
     1561 ggcacgaagg ttcagctcat cgacaagggc aagtggatca agtatccgta tccgcaggac
     1621 cgctgggcga cgtggtattt cccgaacggc ggcttcggcc tcaaaggcca gacgctccag
     1681 gaagcttaca cggatccgac cgatacatcg acgcgcacga actccggcgt attcccgggc
     1741 gacgacacgc cggagaatcg cttctcgatc ttcgcgaatt acaaattcaa ggacgcgctg
     1801 aagggctgga tcgtcggcgc gggcggcgac tggcagtcga agcgcgctta cttctcgggc
     1861 gtcacgcatg gctccggtca ggtgcagacc gacgtcgacg gtgaactgat cgtgctctac
     1921 atgccggagc agtggacgct gaatgcgttt gtgcgcaaag agtggcgccg cgggcgctac
     1981 gaccattccg tgcagctcaa cgtcgacaac gtcatcaacg accgcgacct ctacggccag
     2041 atctacatgg cgccgatctc cgcgcgcatc acgtatcaag tcgggttctg atttcgactc
     2101 gctgcttgcg cctcgcgatc gctatcgcga ggcgcaatgt agcggcaggc ctccgtgcct
     2161 gtctgctcca gaatggaccg atcgctttcg tgacgagcaa cgcaggcagg cagggacgcc
     2221 tgccgctacc tcgatcaccc taacctctcg ttccagcttt ccccgccgtg actacacctt
     2281 ccagcgaacg cctctccttc aaggaaaaac tggcctacgg gctcggtgac acggcttcga
     2341 atttctactt ccaagcgttc aacctgtttc tcgtttacta ctacacggac atcttcgggc
     2401 tgccatccgc ggccgtcggc acgctggtgc tgatcgtgcc gatcgcggtc gcggcgctga
//
LOCUS       DASPLT010000947         1911 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_71506, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000947 DASPLT010000000
VERSION     DASPLT010000947.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1911)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1911)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1911
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_71506"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1152)
                     /locus_tag="VEA63_09840"
     CDS             complement(<1..1152)
                     /locus_tag="VEA63_09840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HYD84346.1"
                     /translation="MAASTPRIQNPILPGFNPDPSIVRVGEDYYIATSTFEWFPGVQI
                     YHSRDLVNWRLLTRPLRRPSQLNMLGDPDSCGIWAPCLTHADGLFWLIYTDVKRYGRT
                     SVGGASGASLRDFHNYLVTCPTIDGDWSDPVYLNSSGFDPSLFHDDDGRKYLVNQLWD
                     HRPGNNRFAGIVLQEYSAKDRKLVGPRKNIFKGTPLGLTEAPHLYKRNGYYYLIVAEG
                     GTAWNHAVTMARSRQIDGPYELHPDTYIMSARHRPDAPLQRAGHADFVETQTGETYLA
                     YLCGRPLHNRGRCTLGRETAIQQMTWGADDWLRTLDGQGIPTLETPAPSYAPHPFPTA
                     PAREDFDSPTLPIDFQWLRTPWPDEIFSLTARPGHLRLYGRETIGSLFKQ"
     gene            complement(1397..>1911)
                     /locus_tag="VEA63_09845"
     CDS             complement(1397..>1911)
                     /locus_tag="VEA63_09845"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HYD84347.1"
                     /translation="YSKLRSLNITPATLIPFIFENDLEGETYGVELAGTVELTDGWRL
                     HAGYNLLKEDLRVVPGGFDVNDTLNETADPQHQVSIRSSFDLPGNVEFDAGFRWVDAF
                     TINNGGNPATVSSYAELDLRLGWRVNDKVELSVVGQNLLHDRHAEYGAPSPLRVEIQR
                     SVYAKLAWRY"
BASE COUNT          356 a          497 c          683 g          375 t
ORIGIN      
        1 ttgcttgaat aaactgccga tcgtttcgcg accgtagagc cgcaggtgtc cggggcgcgc
       61 ggtgaggctg aaaatctcgt ccggccacgg cgtgcgcagc cattggaaat cgatcgggag
      121 ggtcggcgaa tcgaaatcct cgcgagcagg cgccgtggga aagggatgcg gcgcgtagct
      181 cggcgcgggc gtctccaaag ttgggatacc ttggccgtcg agcgtgcgca gccaatcgtc
      241 ggcgccccag gtcatttgtt ggatggcggt ttcgcggccg agcgtgcaac ggccgcggtt
      301 gtgcagcggg cggccgcaaa gataggcgag ataggtttcg ccggtttgcg tctcgacgaa
      361 gtcggcgtgg ccggcgcgtt gcagcggcgc gtcggggcgg tgacgcgcgc tcatgatgta
      421 ggtgtcggga tgcagttcgt aaggtccgtc aatctggcgc gagcgcgcca tggtgacggc
      481 gtggttccaa gcggtgccgc cttccgcgac gatcaggtag tagtatccgt ttcgtttata
      541 cagatgcggc gcttcggtga gaccgagggg agtgcctttg aaaatatttt ttcgcgggcc
      601 gacgagtttg cggtctttgg cggagtattc ctggaggacg atgccggcga aacggttgtt
      661 gccggggcgg tgatcccaga gctggttgac gaggtatttg cggccgtcgt cgtcgtgaaa
      721 cagcgacggg tcgaagccgc tgctgttgag ataaaccgga tcggaccagt cgccgtcgat
      781 cgtggggcag gtgacgaggt agttgtgaaa atcgcggagc gaggcgccag acgcgccacc
      841 gacgctcgtg cgaccgtagc gtttcacatc ggtgtagatg agccagaaaa gtccgtcggc
      901 gtgcgtgagg cacggcgccc agatgccgca ggaatccgga tcgccgagca tgttcaactg
      961 gctggggcgg cggagcggcc gggtgagaag ccgccagttc acgagatcgc gcgagtgata
     1021 aatttgcacc cccggaaacc actcgaacgt cgaagtggcg atgtagtaat cttcaccgac
     1081 gcgaacgatc gacgggtcgg ggttaaaacc gggaaggatg gggttctgaa tgcgcggggt
     1141 ggaggcggcc atggcgaaaa gtagtttgga ggtgcgccga aaagacgagc ggttttggaa
     1201 atcgatgcgc ggtgcgcgcg gcatagcgtg ctcgcctgca aaactcacct ctcgccgcga
     1261 agcacgcacc gcgcgttgta gcgaaacgcg tgagcgtttc gtttatgcga gacccgaaaa
     1321 gctcacgctt ttcgctacgg tatgaatttt gcggggtagc tggaacggcg tcgcgtccgg
     1381 cccccgccgc cttgcttcag tagcgccagg cgagtttggc gtagacgctg cgctggattt
     1441 ccacgcgcag ggggctcggc gcgccgtatt cggcgtggcg gtcgtgcaga agattttgtc
     1501 cgacgacgga gagctcaacc ttgtcgttca cgcgccagcc gagccgcaaa tcgagttcag
     1561 catagctcga gaccgtggcg ggattgccgc cgttgttgat cgtgaacgcg tcgacccagc
     1621 gaaacccggc atcgaactcg acgttaccgg gtagatcgaa ggaggaacga atcgagacct
     1681 ggtgttgcgg gtcggcggtt tcgttgagcg tgtcgttgac gtcgaatccg ccgggaacga
     1741 cgcgaagatc ttccttcagt agattgtaac cggcgtgcag tcgccagcca tcggtgagct
     1801 cgacggtgcc ggcgagttcg acgccatagg tttcgccttc gagatcgttt tcgaaaatga
     1861 acgggatcag cgtcgcgggg gtgatgttca gactgcgaag tttcgaatag t
//
LOCUS       DASPLT010000948         1201 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_154636, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000948 DASPLT010000000
VERSION     DASPLT010000948.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1201)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1201)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1201
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_154636"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..305
                     /locus_tag="VEA63_09850"
     CDS             <1..305
                     /locus_tag="VEA63_09850"
                     /inference="COORDINATES: protein motif:HMM:NF022893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3016 domain-containing protein"
                     /protein_id="HYD84348.1"
                     /translation="LPEGQQLSVTITDVDLAGEVEPWRTGSAHDTRIVKDIYTPRITL
                     DYKLTDASGAVVKEGEARLTDLNFNMKLHANRSDPRVHEKALIDDWVRAEFRKAKK"
     gene            389..781
                     /locus_tag="VEA63_09855"
     CDS             389..781
                     /locus_tag="VEA63_09855"
                     /inference="COORDINATES: protein motif:HMM:NF013633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein"
                     /protein_id="HYD84349.1"
                     /translation="MDTPGSAESGARIDKWLWAVRLFKTRAAASDACRAGSVEIASHA
                     VKPSRDVRAGDLIIVRQGLIIRTLRVLASPRSRVGAKLVPTYCEDLTPAAEFEKVREQ
                     RVQHVLAREKGSGRPTKRDRRALDRLFE"
BASE COUNT          227 a          412 c          363 g          199 t
ORIGIN      
        1 cactgccgga aggccagcag ctcagcgtca ccatcaccga cgtcgatctc gccggtgaag
       61 tggagccgtg gcgcaccggc tcggcgcacg acacccgcat cgtcaaggac atctacacgc
      121 cgcgcatcac gcttgattac aagctcaccg acgccagtgg cgccgtcgtg aaggaaggcg
      181 aagcgcggct gacggacttg aacttcaaca tgaagcttca tgccaaccgc agcgatccgc
      241 gcgtgcacga gaaggccttg atcgatgact gggtccgcgc ggaatttcga aaagcgaaaa
      301 agtaaccgct gatcggccgg cgtattcgcc gaaacacgtt tgctcgcgac cgcgaatcgt
      361 ccagattcgc ggtcgcttgc ttttaccgat ggacactccc ggcagcgccg aatccggcgc
      421 gcggatcgac aagtggcttt gggccgtgcg cctgttcaag acgcgcgccg ccgccagtga
      481 cgcctgccgc gccggcagcg tcgaaatcgc cagtcacgcc gtgaagccgt cgcgggacgt
      541 ccgagctggc gacctgatca tcgttcgcca gggactcatc atccgcacgc tgcgcgtcct
      601 cgcttcaccg cgctcacgcg tcggcgccaa gctcgtgcca acgtattgcg aagacctgac
      661 gcctgccgcc gaattcgaga aagtccgcga acagcgagtg cagcatgtgc ttgcgcgcga
      721 aaaaggctcc ggccggccca ccaaacgcga ccgccgcgcg ctcgatcggt tgttcgaata
      781 agtgccaata agcgcgggcg cgccgcagcg aggccggccc gattttgcaa gctacgtcca
      841 attacaagct agggcttaaa ccctgagcta aaccgtgccg gcaaacgtgc cgacaagtct
      901 ggcgcatgcc catccaagat gcgatcggcc tcgccgtctc catgacggca cggtccagcc
      961 gcgtcgtggc gaaatctttc gccacgaccg cgcgcgcgta tgcgggcatg tcggtggatg
     1021 gacgttcctt tgcgggaggc cgcgcgtgag cgaatcggtc caaaccgccg cgatcacggc
     1081 gttcgccgtc tcgccgccag tcgccgccgg gcgccggccc tgccaagtcg ccacgttgtt
     1141 cgccacttac gagcaccgcg tattcgtccg aacgaaccgc ctgtttcgct ggttcctgct
     1201 c
//
LOCUS       DASPLT010000949         1324 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_298298, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000949 DASPLT010000000
VERSION     DASPLT010000949.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1324)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1324)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1324
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_298298"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(327..701)
                     /locus_tag="VEA63_09860"
     CDS             complement(327..701)
                     /locus_tag="VEA63_09860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD84350.1"
                     /translation="MTPTETLHQHQQLCDELHQCALEENRFLRQHQRPLSSEFVDRKR
                     TLLTKLDASLLALRAVPAGRLKEPETRDLLEQTRARILQILQLDKENEQLLLRFSLAG
                     PRPASTGMPSSALLQKIYSRIS"
     gene            complement(658..>1324)
                     /locus_tag="VEA63_09865"
     CDS             complement(658..>1324)
                     /locus_tag="VEA63_09865"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HYD84351.1"
                     /translation="PSDRARAHFKAGYALHLAEDHEGAVVSLRAFLEQWPDDENVPEA
                     RFLLATTLRKLNRAQESLTATLDLLRAERSRSNADAKRWSYWQRRTGNQLANDFFQSG
                     DVHNALAIYTSLAALATEPNWRMPVTYQIALCYERLGDLDRACQTYRAIIAEAGESPS
                     PDTADIARMAASRLAHVDWRGNVDRQVSALFDSTTGQIPPTSQESLANDANRNPASAP
                     AAL"
BASE COUNT          219 a          372 c          477 g          256 t
ORIGIN      
        1 tctgctcgac gtgagcgcac cggcggtgcc ggcgcgtgcg cggatgcctg cggtgttcgg
       61 tttcgcgcgg taggatcgaa gctcgcgtgg ccgttccgga aaccctgtcc gcggcccaga
      121 cgaacttgca gcggttcacc ccactaagcc ggacgtgtgc cgttgaaggt ggagcctgac
      181 ggcgtcctcg gactggttcg cgagcggagt tgcctcaagc ccgatgagga catcaggctc
      241 cacctcctca ctacgatttt gcgagcgcaa cgtggttcaa ctgcacactt ctgttgcagt
      301 gatcccgcgt gctggcgcgc gcgacactat gaaatccgag aatagatttt ctgcagaagc
      361 gcggacgaag gcataccggt gctcgcgggc cgcggaccgg cgaggctgaa gcgaagcagc
      421 agttgttcgt tttccttgtc gagctgtaga atctggagga tgcgcgcgcg cgtctgctcg
      481 agcagatcgc gggtttcagg ctccttcaac cgacccgcgg gcacggcgcg cagcgcgaga
      541 agcgaggcat caagcttggt cagcagcgtc cgtttgcggt caacgaattc gctggaaagc
      601 gggcgctggt gctggcggag aaaccggttc tcttcgagcg cgcattgatg gagctcgtca
      661 cagagctgct ggtgctgatg cagggtttcg gttggcgtca ttggcgaggg actcctgaga
      721 agtgggtgga atttgaccgg tggtcgaatc aaagagtgcg gacacctggc ggtcgacgtt
      781 gccgcgccaa tcgacatgtg cgagacgcga cgccgccatg cgggcgatgt ccgccgtgtc
      841 gggcgacgga gattcgccgg cttcggcgat gatcgcgcgg taggtttggc aggcgcggtc
      901 gagatcgccg agtcgctcgt agcacaaggc gatttgatac gtgaccggca tgcgccagtt
      961 gggctcggtg gcgagcgcgg cgaggctcgt gtaaatcgcg agcgcattgt gcacatcgcc
     1021 actctggaag aaatcgttgg cgagttggtt gcccgtgcgg cgctgccagt agctccagcg
     1081 tttcgcgtcg gcgttggagc gcgagcgttc ggcgcgcaat agatcgagcg tggcggtcag
     1141 cgactcctgg gcgcggttca gtttgcgcag tgtagtcgcg aggagaaagc gggcttcggg
     1201 cacgttctcg tcgtccggcc actgttcgag aaacgcgcgc aggctgacga cggcgccttc
     1261 atgatcctcg gcgaggtgca gcgcgtagcc ggctttgaaa tgggcgcgcg cgcggtcgct
     1321 cggc
//
LOCUS       DASPLT010000950         3001 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_34961, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000950 DASPLT010000000
VERSION     DASPLT010000950.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 3001)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3001)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3001
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_34961"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(248..>3001)
                     /locus_tag="VEA63_09870"
     CDS             complement(248..>3001)
                     /locus_tag="VEA63_09870"
                     /inference="COORDINATES: protein motif:HMM:NF037015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HYD84352.1"
                     /translation="IVARIASEQHTSEWAKVGVMFRDNLDPGSRYCFLALTPDRPTGT
                     DRGFLFEYRDTVDDDAAQNAAVAGHVAPQWLKLVRAGNSFTAFRSTDGTNWSAVGTAQ
                     NVPLNATGLVGLAVCSHANPSALNTSTFTDVSVTTDTSGPTVSITQPADMATVSGTSV
                     QIAASASDSSGVANVKFYVDSSTQIGPTDSTPPYETTWDTTGVSNAQHVLTAQATDIV
                     GNPNTSGGIFVTVNNSTTLPSGWTASDIGDVGAPGSTSYTSGTYTIAGSGANIWNAAD
                     EFQFARTSITGDCTIVARVASVDNTATWSKAGIMIRETDDPDSKYVFVLMTPNNGLLF
                     EMRAGAAPSSSTQPAAVSTIDPPQWLKLTRSGNTFTAYYSSNGSSWMQAGSPQTVSMS
                     GNVLAGLAVCSVSDGNLCTAVFDNVDIAAGAGAESFNPAFPRLGIYTISGSQTYPEEM
                     QEKLARFHVVFMGGNWEGWENNRGYTREHVVSEIKRLSQIGTKVFQYVIFEAVWDDDT
                     NHQDAWNPTWKQIAENNNWFVYASGTSGPRIGSDFRPNDQWVINPTHYTTKDTSVTPS
                     LWPFESAAKFAYDLFVNPSSSFAAGNKAPSLDGFFLDNTFWTPRMSGDWNRDSSNSND
                     HDSNPNTPGIPDHQFNEVREQFRIGEKDFYDKIRALWPGKIFVGNNTDFPRAVEFTGG
                     TTPPPVLDWSDLSVLYPLNQVLQGGVCEAYIGKNWSFEDDYSTASVIARYGYTMDAVA
                     APKLQLFEQSDLSTTGTDYADATAYRAMRYGLAISLMNDGYYNAEGSNHSGSASSILW
                     FDEFSVYNGEGQAIDSAHVFDGIGYLGHPIAGEHGAVQTEPRWPDKGTHGVWAREFEN
                     GIVLLNPKGNGSRTITVGSSGADLSDTEWKRIDGVSSVNDGSNVTSDITLDPRDGLIL
                     LRR"
BASE COUNT          514 a          850 c         1005 g          632 t
ORIGIN      
        1 gaggaagcag gatgccgcga ggtgttgctg cgtttcatgc ggatatagca gttcaaaaat
       61 tatatagccg agtttttggc cgcaaaaagg cgcaaaaggc gcaagagccg gagagtcctc
      121 caagtcattt ttgcgcattc tgtgcctttt tgcggctaca cttatgattg atttgctgtg
      181 gatgctgcaa aaaacccgcc gggggtgccg gcgggtcgta acgttgtgaa cgagttggtg
      241 gtggtgctca gcgccgcagc agaatcaggc cgtcgcgcgg gtcgagcgtg atgtcgctcg
      301 tgacgttcga gccgtcgttg accgaactga cgccgtcgat gcgtttccat tcggtgtcgc
      361 tcaaatcggc gccgctcgag ccgacggtga tcgttcgcga accattgcct ttcggattca
      421 gcaacacgat gccgttctca aactcgcgcg cccagacgcc gtgcgtgccc ttgtcgggcc
      481 agcggggctc ggtctgcacg gcgccatgtt cgccggcgat cggatgaccg aggtagccga
      541 tgccgtcgaa gacatgcgcg gagtcgatcg cctggccctc gccgttatag accgagaact
      601 cgtcgaacca caggatgctg cttgcgctgc cggagtggtt ggaaccctcg gcgttgtagt
      661 agccgtcgtt catcagggag atcgcgaggc cgtagcgcat cgcgcggtag gctgtggcat
      721 cggcgtagtc ggtgccggtg gtcgacaggt cgctttgctc gaagagctgc agcttcggcg
      781 cggcgaccgc gtccatggtg tagccgtagc gcgcgatcac gctggcggtg ctgtagtcgt
      841 cctcgaagga ccagttcttg ccaatgtagg cttcgcacac gccaccctgc agaacttgat
      901 tgagcgggta gaggacggaa agatcgctcc aatcgagaac gggcggcgga gtcgtgcctc
      961 cggtgaattc gaccgcgcgc gggaaatcgg tgttgtttcc gacgaagatc tttcccggcc
     1021 agagcgcgcg gatcttgtcg tagaaatctt tttcgccgat gcggaattgc tcgcggacct
     1081 cgttgaactg gtgatccgga atgccgggcg tgttcgggtt gctgtcatga tcgttggagt
     1141 tgctgctgtc gcgattccaa tcgccgctca tgcggggcgt ccaaaacgtg ttgtcgagga
     1201 agaagccgtc gagcgacggc gccttgttgc ccgccgcaaa gctgctgctc gggttgacga
     1261 agaggtcgta ggcgaacttc gcggcggatt cgaacggcca gaggctcggg gttaccgacg
     1321 tgtctttcgt ggtgtagtga gtcgggttaa tgacccattg gtcattaggt ctgaagtcgc
     1381 tcccgatccg cgggccgctc gtgccggatg cgtagacgaa ccagttgttg ttttcggcga
     1441 tctgcttcca ggtgggattc cacgcatcct gatggttcgt atcgtcatcc caaacggctt
     1501 cgaagatcac gtattgaaaa accttcgtgc cgatctgcga cagccgtttg atttcactca
     1561 cgacatgctc gcgcgtgtag ccgcggttgt tctcccagcc ttcccagttg ccgcccatga
     1621 agacgacgtg gaatctcgcc agcttctcct gcatttcctc gggatacgtc tggcttccgc
     1681 tgatggtgta gatgccgaga cgcgggaacg caggattgaa gctctccgcg ccggcgccgg
     1741 ccgcgatgtc gacgttgtcg aacaccgcgg tgcacaggtt gccatcggag acgctgcaga
     1801 cggcgaggcc agcgagcacg ttgccggaca tcgaaacggt ttgcggcgag ccggcctgca
     1861 tccacgacga gccgttcgag gaataatacg cggtgaacgt gttgcccgag cgcgtgagtt
     1921 tcagccattg cggcggatcg atcgtggaaa ccgccgcggg ttgcgtcgaa ctcgacggcg
     1981 cggcgccggc acgcatctcg aacagcaggc cgttgttcgg cgtcatgagc acgaacacgt
     2041 atttcgaatc gggatcgtcc gtctcacgaa tcatgatacc ggctttcgac cacgtcgcgg
     2101 tgttgtcgac cgaagccacg cgcgcgacga tcgtgcaatc tccggttatc gacgtgcgcg
     2161 cgaattgaaa ctcgtccgcg gcgttccaga tgttcgcgcc ggaaccggcg atcgtgtagg
     2221 tgccgctcgt gtaactcgtg ctgccggggg cgccgacgtc gccgatgtcg ctcgcagtcc
     2281 agcccgaggg cagggtggtg ctgttgttca cggtgacgaa gattcctccc gacgtgttcg
     2341 ggttgccaac gatgtcggtg gcttgcgcgg tcagcacgtg ctgggcgttc gagacgccgg
     2401 ttgtgtccca cgtcgtttca tacggcggcg tgctgtcggt cgggccgatc tgcgtggagc
     2461 tgtcgacgta gaatttcacg ttcgcgacgc cggagctgtc cgacgcgctg gccgcgatct
     2521 gcacgctcgt gccggacacg gtcgccatgt ccgccggctg cgtgatcgaa accgtgggcc
     2581 cggaggtgtc ggtcgtgacc gacacatcgg tgaacgtcga cgtgttcaat gcagaggggt
     2641 tcgcgtggct gcagacggcg agtccgacga gaccggtggc gttgagagga acgttctggg
     2701 cggtgccgac ggcgctccag tttgtgccat cggtcgaacg gaacgcggtg aagctgttgc
     2761 ccgcacgcac aagcttcaac cattgcgggg cgacatggcc ggcgacggcg gcgttttgtg
     2821 ctgcgtcgtc atcgacggtg tcgcggtatt caaaaagaaa accgcgatcg gtgccggtcg
     2881 gccggtcggg ggtgagagcg agaaaacaat agcgtgagcc cgggtcgagg ttgtcgcgga
     2941 acatcacgcc cacctttgcc cactcgctgg tgtgttgctc gctcgcgatg cgcgccacga
     3001 t
//
LOCUS       DASPLT010000951         1065 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_112926, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000951 DASPLT010000000
VERSION     DASPLT010000951.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1065)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1065)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1065
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_112926"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1065
                     /locus_tag="VEA63_09875"
     CDS             <1..>1065
                     /locus_tag="VEA63_09875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012377320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-binding family 6 protein"
                     /protein_id="HYD84353.1"
                     /translation="GQGVLDALALQPDRKLAFIHRQHEAGAKEIAKQFAPLVEHPNVD
                     FIYSFKYAEAHALSSTKQNFHENFLKDVGDQPVLWTLRNDDAFYFRWGAPDFVRQFIR
                     AMPAHPTRGFYYGSDQWVWGRDFLDLERVPDAARQLEHEKHGYHWLLWGRLGYDPDLS
                     NDRLQSILAGRFNLSADTAARLFAAWQDASMIYPLTTGFHWGRFDFQWYIEASKSRPD
                     PARTPSGFHDINRFISLPPHPESSNVSIPDYVDAVRRNVAPAGTTPLQVAEQLHRHAD
                     RAFAAVDTIVRGDRSAGHSDSADELRRTLEDIRAMAHLGKYYAHKIRAATELALFRAT
                     LDRAHHTTLAAELNQSAYQWR"
BASE COUNT          202 a          406 c          271 g          186 t
ORIGIN      
        1 ggccaaggcg tgctcgacgc gctcgccctc cagcccgacc gcaagctcgc gttcatccac
       61 cgccagcacg aagccggtgc caaggaaatc gcgaagcagt tcgccccgct cgtcgaacac
      121 ccgaacgtcg atttcatcta cagcttcaaa tacgccgagg cgcacgcgct cagttccacg
      181 aagcagaatt tccacgaaaa ctttctcaag gacgtcggcg accagcccgt gctttggaca
      241 ctgcgcaacg acgacgcgtt ctacttccgc tggggcgcgc ccgatttcgt ccgccaattc
      301 atccgcgcca tgcccgcgca tccgacgcgc ggcttctact acggctccga tcaatgggtg
      361 tggggccgcg atttcctcga tctcgagcga gttccagacg ctgcacgcca gctcgaacac
      421 gaaaaacacg gctatcattg gctgctctgg ggccgcctcg gctacgatcc cgacctctcc
      481 aacgatcgcc tgcaatcgat cctcgcagga cgtttcaacc tttccgccga caccgccgca
      541 cggctcttcg ccgcgtggca ggacgcgtcg atgatttacc cgctgacgac tggctttcac
      601 tgggggcgct tcgatttcca atggtatatc gaagcgtcga aaagccgccc cgatcccgcg
      661 cgcacgccga gcggttttca cgacatcaac cgtttcatct cgctgccgcc acacccggaa
      721 agcagcaacg tctcgattcc cgactacgtc gatgctgttc gacgcaacgt cgcacccgcc
      781 ggcaccacgc cgctgcaagt cgccgagcag ctccaccgcc acgccgaccg cgccttcgcc
      841 gccgtcgaca cgatcgttcg cggcgatcgt tcggccggcc actctgattc cgccgacgag
      901 ctccgtcgca cactcgaaga catccgtgcg atggctcacc tcgggaaata ctacgcccac
      961 aaaatccgcg ccgccaccga gctcgctctc ttccgcgcca cgctcgaccg cgctcatcac
     1021 acaaccctcg ccgccgagtt gaaccaatcc gcgtatcagt ggcgc
//
LOCUS       DASPLT010000952         1738 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_298308, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000952 DASPLT010000000
VERSION     DASPLT010000952.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1738)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1738)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1738
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_298308"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..484
                     /locus_tag="VEA63_09880"
     CDS             <1..484
                     /locus_tag="VEA63_09880"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007359370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HYD84354.1"
                     /translation="YMAKLKEKGEAETASFLDEVKKKAGVIVLPSGLAYEVETQGEGA
                     APKAEDTVRVHYTGTLVNGTVFDSSRERGEPVEFALNEVIPGWTEGLQKVSKGGKIKL
                     YIPPQLAYGDEGRPGIPPASTLVFDIELLDINPPAAAAAPGAEGQSPEIQFPTPAEKK
                     "
     gene            complement(681..>1738)
                     /locus_tag="VEA63_09885"
     CDS             complement(681..>1738)
                     /locus_tag="VEA63_09885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="low specificity L-threonine aldolase"
                     /protein_id="HYD84355.1"
                     /translation="NTAGICPEAWTALHEANTGHAPSYGDDMWTSRAKQQFRELFETD
                     CDVFFVFNGTAANALALAVCCRSPHTRVICHDYAHIDTDECGAPEFFTRGAKLNPIAG
                     VNGKLHPADVERALHRGHDVHYPKPAALSLTQSTEWGTVYTPAELRELASLAHAHGLA
                     VHLDGARFANAMAALQQPGTPNPKLGTLNPKPGTASPADATWRAGVDVLCFGGTKNGM
                     HNAEAVVFFNRDLAREFDYRVKQSGQLSSKMRFAAAQWSAMLHDSAWLRHAAHANAQA
                     RKLANALRELPSLRLIAEPEVNGVFVEMPQALYDALTARGWHFYRFIGEHGYRLMCSW
                     ATTDAQIDGFIADLRRA"
BASE COUNT          302 a          473 c          646 g          317 t
ORIGIN      
        1 ctacatggcg aagctgaagg aaaagggcga agcggagacg gcctcgtttt tggatgaagt
       61 gaaaaagaag gcgggcgtga tcgtgctgcc gagcggcctc gcttatgagg tcgagacgca
      121 gggcgaaggc gcggcgccga aggcggaaga cacggtgcgc gtgcactaca cgggcacgct
      181 ggtgaatggc acggtgttcg acagctcgcg cgaacggggc gagccggtcg agttcgcgct
      241 caacgaggtc attcccggct ggaccgaagg cctgcagaag gtttccaagg gcggcaaaat
      301 caaactctat attccgccgc aactcgctta cggcgacgaa ggccgaccgg gcattccgcc
      361 ggcgtccacg ctggtgttcg acatcgaact gctcgacatc aacccgcctg cggccgccgc
      421 ggcgcccggc gccgagggcc aatcgccgga gattcagttc ccgacgccgg cagagaagaa
      481 gtaaattggt gtagcgcgtt cgcggatacg agcgcgcggc gcccacgttg cgacgattgt
      541 gacgaagccc tggcgaaagc cggggctttt tgctgatctc gtgtggtgtc ctgcaggtat
      601 ctttaacaat tcggcattct tacactgcac ggacgcctcg gagaggcgtc cctaccggcg
      661 ctgccgcgcc acttaccata ctaggccctg cggaggtcag cgatgaaacc gtcgatttgg
      721 gcgtcggtcg tggcccagga acacatgaga cgatagccgt gttcgccgat gaatctgtag
      781 aagtgccagc cgcgcgcggt gagcgcatcg tagagagctt gcggcatctc gacgaagacg
      841 ccgttcacct cgggttcggc gatgagacgg agcgagggga gttcgcgcaa ggcgttcgcg
      901 agcttgcgcg cttgggcgtt ggcgtgggca gcgtgtcgga gccaggcgct gtcgtggagc
      961 attgcgctcc attgggcggc ggcgaaacgc attttggaag agagctgtcc gctttgcttc
     1021 acgcggtagt cgaactcgcg ggcgaggtcg cgattgaaga aaacgacggc ttcggcgttg
     1081 tgcatgccgt ttttcgtgcc gccgaaacag agcacgtcga cgccggcgcg ccaggtggcg
     1141 tcggcgggcg acgcggttcc gggtttcggg tttagggttc cgagtttcgg gttcggagtt
     1201 ccgggttgtt ggagcgcggc catcgcgttg gcgaagcggg cgccgtcgag gtgcacggcg
     1261 aggccgtggg cgtgggcgag cgaggcgagt tcgcgaagct cggcgggcgt gtagacggtg
     1321 ccccactcgg tggattgagt gagggagagc gcggcaggtt tgggatagtg aacatcgtgg
     1381 ccgcgatgga gcgctcgctc gacgtcggcc ggatggagtt tcccgttcac gcctgcgatc
     1441 gggttgagct tggcgccgcg ggtgaaaaat tccggcgcgc cgcattcgtc ggtgtcgatg
     1501 tgggcgtagt cgtgacagat aacgcgcgtg tgcgggctgc ggcagcaaac ggcgagggcg
     1561 agcgcgttgg ccgcggtgcc gttgaacacg aagaagacat cgcagtcggt ttcgaagagc
     1621 tcgcgaaatt gctgcttcgc gcgtgaggtc cacatgtcat cgccgtagct cggcgcgtgg
     1681 ccggtgttgg cttcgtggag cgcggtccag gcctccggac aaatgccggc ggtgttgt
//
LOCUS       DASPLT010000953         1645 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_92718, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000953 DASPLT010000000
VERSION     DASPLT010000953.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1645)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1645)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1645
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_92718"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            8..130
                     /locus_tag="VEA63_09890"
     CDS             8..130
                     /locus_tag="VEA63_09890"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HYD84356.1"
                     /translation="MSTHAIKPVISDVMPFERAAEAFALMERGGQFGKIVLRVK"
     gene            209..1276
                     /locus_tag="VEA63_09895"
     CDS             209..1276
                     /locus_tag="VEA63_09895"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HYD84357.1"
                     /translation="MSENPNAYVCIMAGGSGERFWPMSRQRTPKHLLKLFADRTLLED
                     TVRRLDGVVPRANIFVLTNEVQLDGARAALPFLAPEQIVAEPAKRDTAPAAALATGLV
                     RARNPNAVMALLPADALIKNHARLAQQLAAALARAATTDALLTFAIKPTYPSPGFGYL
                     EMGEELARSAEIGTIRRVTRFVEKPDLATAERYVASGNYAWNAGMFIWRVGAFLAETE
                     KHAPELAAFVKGFPAGDPKAYLAEKFPALPKISVDYAVMEKAAAVETILAEYDWDDVG
                     MWTSLPPHLQADEAGNAVRGLVVGVGAEKNVAISNGRVIALVGVKDLVVVETADAVLV
                     CHRDAVQDIKKLMPLLPKDLV"
     gene            1482..>1645
                     /locus_tag="VEA63_09900"
     CDS             1482..>1645
                     /locus_tag="VEA63_09900"
                     /inference="COORDINATES: protein motif:HMM:NF012670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD84358.1"
                     /translation="MTLQTPLLAKAANQARGLAIDAVHACSSGHLGLPLGAAEIGAVL
                     YGHALSHNPER"
BASE COUNT          279 a          463 c          585 g          318 t
ORIGIN      
        1 gcagtatgtg agcacgcatg cgatcaagcc ggtgatcagc gacgtgatgc cgttcgagcg
       61 tgcggcggag gcctttgcgc tgatggagcg gggcgggcag ttcggaaaaa ttgtgctgcg
      121 cgtgaaatag ggacgcgcgg caaccgagtc gttcgcgttg gttcaggctt ggccttggtt
      181 cgtccggctg agaatttgat tcttttctat gagcgaaaat ccgaacgcct acgtttgcat
      241 catggccggt ggcagcggcg aacgcttctg gccgatgagc cggcagcgca caccgaagca
      301 tctgctgaag ctgtttgcgg accgcacgct gctcgaggac accgtgcgcc ggctggatgg
      361 cgtcgtgcca cgcgcgaata ttttcgtgct gacgaacgag gtgcagctcg acggtgcgcg
      421 ggccgcgctg ccgtttctcg ccccggagca gatcgtggcg gagccggcga aacgcgacac
      481 ggcgccggcg gcggcgctgg cgacggggct cgtgcgcgcg cgaaatccga acgcggtgat
      541 ggctctgctg ccggcggatg cgttgatcaa gaatcacgcg cggctcgcgc agcaactcgc
      601 ggccgctctg gcccgcgcgg cgacgacgga tgcgttgctg acctttgcga tcaagccgac
      661 gtatccatct cctggattcg gttatttgga gatgggcgag gagctcgcgc gttcggcgga
      721 gatcggcacg attcggcgcg tgacgcgttt tgtcgagaaa ccggatctcg cgacggcgga
      781 gcgctacgtc gcgagcggca actacgcctg gaatgcggga atgttcatct ggcgggtcgg
      841 cgcgttcctg gcggaaacgg aaaagcacgc gccggagctg gcggcgtttg tgaaagggtt
      901 tcccgcgggc gatccgaagg cgtatctggc ggagaaattt cccgcgttgc cgaagatttc
      961 ggtcgattac gcggtgatgg agaaagcggc tgcggtggag acgattttgg cggaatacga
     1021 ttgggacgac gtcggcatgt ggacgtcgct gccgccgcat ttgcaggcgg atgaggcggg
     1081 caatgctgtg cgcgggctgg tggtcggcgt cggcgcggaa aaaaacgtcg cgatcagcaa
     1141 cggccgggtc atcgcgctcg tgggcgtgaa ggacttggtc gtggtggaaa ccgccgatgc
     1201 ggttttggtg tgtcaccgcg atgcggtgca ggacatcaag aagctgatgc cgctgctgcc
     1261 gaaagatctg gtgtaggtga aattgttagc ggcggaagtg ttcgcatgga gcccggcatc
     1321 cgccgggctt ttttcaaaca atccgacgga cgtcggattc caccccgtcg gtctactgaa
     1381 tgactgcgcg ggcgatttgc cggcgggcga acgctgtcac aaggtggatt tttgccttcc
     1441 cgcaccgccg ggcgcgccct agtgctaacc tttctcatcc aatgacgctc caaactcctc
     1501 tcctcgccaa agccgccaat caggcgcgcg gcctcgccat cgatgcggtg cacgcgtgtt
     1561 cttcgggaca cttgggtctg ccgctcggtg cggccgagat cggcgccgtg ctctacgggc
     1621 acgcgctttc gcacaacccg gagcg
//
LOCUS       DASPLT010000954         2509 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_71589, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000954 DASPLT010000000
VERSION     DASPLT010000954.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 2509)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2509)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2509
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_71589"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1242)
                     /gene="hydA"
                     /locus_tag="VEA63_09905"
     CDS             complement(<1..1242)
                     /gene="hydA"
                     /locus_tag="VEA63_09905"
                     /EC_number="3.5.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004157 - dihydropyrimidinase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropyrimidinase"
                     /protein_id="HYD84359.1"
                     /translation="MSLLIKNGEIVTPSQRYTADIYCEGETITRIDRNLSAPAGTTIV
                     DATGKYVFPGFIDPHVHIYLPFMGTYSKDTYETGSQAALVGGTTTLIEMCCPARKDDA
                     LAAFDLWMAQAAGKSACDFSFHMGVTKFDAGTEAQLREIVARGISSFKIFLAYKDAFG
                     IHDTELYRTLKLAKELGVVVTAHCENETLVAERSRELLAAGKTDPAQHHESRPPRVEA
                     EGVHHLMTFAELTGAATYIVHLSCEEALREALAARQRGVRVGIETLIQYLTLDKTYAE
                     RPDFEGAKYVMSPPLRDVRNQSILWNALRDGLIQTVATDHAPFDFEKQKPMGRDDFTK
                     IPNGIPSLEERITLLYTYGVRRGRIDLPTLVNVASTAVAKQFDLFPRKGAIQPGADAD
                     LVVFDPDYRGTISARTQHMNVD"
     gene            complement(1378..>2509)
                     /gene="mmsA"
                     /locus_tag="VEA63_09910"
     CDS             complement(1378..>2509)
                     /gene="mmsA"
                     /locus_tag="VEA63_09910"
                     /EC_number="1.2.1.27"
                     /inference="COORDINATES: protein motif:HMM:TIGR01722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004491 - methylmalonate-semialdehyde
                     dehydrogenase (acylating, NAD) activity [Evidence IEA];
                     GO_process: GO:0006574 - valine catabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CoA-acylating methylmalonate-semialdehyde
                     dehydrogenase"
                     /protein_id="HYD84360.1"
                     /translation="GDTLENLARGVDCETLHQPLGVCVGITPFNFPAMVPLWMFPLAL
                     ACGNTFVLKPSEKVPLTANLLGQLLEQAGVPKGVFNIVHGGRACVDALLTHPKVRAIS
                     FVGSSPIAKYIYETGTRHGKRVQANGGAKNHIVIMPDADVPKTVEALATAAFGCAGER
                     CMAGSTAIAVGAAGNRLLPSLVAAARNITVGRTDQPSAPAQPDMGPVITAQHRARVLE
                     LVASGEKDGARIIADGRDVKVPDAPRGFYLGATIVDGVEANMTLAREEVFGPVLNVMR
                     MDDLDRAIETANRSAFGNGAAIFTASGHAAREFKHRVKAGMVGINVGVPATMAMFPFT
                     GWDDSFYGDLHIQGREAVQFYTQQKVVTTRWFGGEVGDVWKK"
BASE COUNT          439 a          667 c          898 g          505 t
ORIGIN      
        1 atcgacgttc atgtgctggg tgcgagcgga aatcgtgccg cgataatccg gatcgaacac
       61 gacgaggtcg gcgtcggcgc cgggctggat tgcgcccttg cgcgggaaga gatcaaactg
      121 cttcgccacc gcggtgctcg cgacgttgac gagcgtgggc agatcgatgc ggccgcggcg
      181 gacgccgtag gtgtagagca gagtgatgcg ctcttcgagc gacggaatgc cgttggggat
      241 tttggtgaaa tcgtcgcggc ccatcggctt ttgtttttcg aaatcgaagg gcgcgtgatc
      301 ggtcgccacg gtttgaatca ggccgtcgcg gagcgcgttc cagagaattg actggttgcg
      361 gacatcgcgg agcggcggcg acatcacgta tttggcgcct tcgaagtccg gacgctcggc
      421 gtaggtttta tcgagcgtga gatattggat gagcgtctca atgccgacgc gcacgccgcg
      481 ctggcgggcc gcgagcgctt cacgcagcgc ttcttcgcaa ctcaggtgaa cgatgtaagt
      541 ggcggcgccg gtgagctcgg cgaacgtcat caagtggtgc acgccctccg cttcgacacg
      601 gggcggacga ctctcgtgat gctgggcggg gtcggtcttg ccggcggcga gaagttcgcg
      661 ggagcgctcg gcgacgagcg tttcgttttc acagtgcgcg gtgacgacga cgccgagctc
      721 cttcgcgagt ttgagcgtgc gatacagttc ggtgtcgtgg atgccgaagg cgtctttgta
      781 agcgaggaag attttaaacg aactgatgcc gcgagcgacg atttcgcgga gctgcgcttc
      841 ggtgccggcg tcgaatttcg tgacgcccat gtggaacgag aaatcacagg cagatttgcc
      901 ggcagcctgc gccatccaga gatcgaacgc ggcgagggcg tcatccttgc gtgcggggca
      961 gcacatctcg atgagcgtgg tggtgccgcc gacgagcgcg gcctgggagc ccgtttcgta
     1021 agtatctttt gaatacgtcc ccatgaaggg caggtagatg tggacgtgcg gatcgatgaa
     1081 gccggggaaa acgtatttgc cggtggcatc gacgatggtg gtgccggccg gcgcggagag
     1141 gttgcgatcg atgcgggtga tcgtctctcc ctcgcagtaa atgtcggcgg tgtaacgctg
     1201 ggagggcgtg acgatttctc cgtttttaat gagtagagac attttttaag ctccaagcac
     1261 caagatccaa gctccagaga agcttcagga ttcaaacttc aattgacgga atcgcggtgg
     1321 gcttggtgtt tgaaggctgg tgtttcattg gaggttggat gttggagctt ggagctttta
     1381 tttcttccaa acatcgccga cttcgccgcc gaaccatctt gtcgtgacga ccttttgctg
     1441 ggtgtagaac tgcacggctt cgcggccctg aatgtgaaga tcgccgtaga aagaatcgtc
     1501 ccagccggtg aaggggaaca tcgccatcgt ggcggggacg ccgacgttga tgccgaccat
     1561 gccggctttc acgcggtgtt tgaactcgcg cgcggcgtgt ccgctcgcgg tgaaaatcgc
     1621 ggcgccattg ccgaaggcgg agcggttggc cgtttcgatc gcgcggtcga gatcgtccat
     1681 gcgcatgaca ttgaggacgg ggccgaacac ttcctcgcgg gcgagcgtca tgttggcttc
     1741 gacgccgtcg acgatggttg ctccgagata aaaaccgcgc ggggcgtcgg ggactttgac
     1801 gtcgcggccg tcggcgatga tcctggcgcc gtctttctct ccgctcgcaa cgagctcgag
     1861 cacgcgggcg cgatgctgcg cggtgatgac ggggcccatg tcgggctgag ccggggcgga
     1921 cggctggtcg gtgcgaccga cggtgatgtt gcgcgccgct gcgacgagcg agggcagcaa
     1981 gcgattgcca gcagcgccga cggcgatggc ggttgagccg gccatgcagc gttcaccggc
     2041 gcagccgaac gcggcggtgg cgagcgcttc gacggtctta ggcacgtcgg catcgggcat
     2101 gatgacgatg tggtttttcg cgccgccgtt ggcttggacg cgtttgccgt ggcgcgtgcc
     2161 ggtttcgtag atgtatttgg caattggaga cgagccgacg aaggagatgg cgcgaacctt
     2221 cgggtgggtg agcaacgcgt cgacgcaggc gcgtccgcca tgcacgatat tgaacacccc
     2281 cttgggcacg ccggcttgct cgaggagctg accgagaagg ttggcggtga gcgggacttt
     2341 ttcgctcggc ttcaggacga acgtgttgcc gcacgcgagc gcgagcggga acatccagag
     2401 cggaaccatc gcggggaagt tgaacggcgt gatgccaacg cagacgccga gcggctggtg
     2461 aagggtttcg caatcgacgc ctcgcgcgag gttttcgagc gtgtcgccg
//
LOCUS       DASPLT010000955         1053 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_196606, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000955 DASPLT010000000
VERSION     DASPLT010000955.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1053)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1053)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1053
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_196606"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1053
                     /locus_tag="VEA63_09915"
     CDS             <1..>1053
                     /locus_tag="VEA63_09915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007361065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD84361.1"
                     /translation="LIAGEERTKEVFGDYVSIYDYQQSVEDGATVPLFYENRTPELEL
                     TNPTLNEDIYDIIEEAALDEAQEERLAQVLGKQYHIITRDDRLDTVAQDIVRHFLGRG
                     FQGKAIVVSIDKATALRMYDKVERELMMEAKRLKAEWRKLEDLKRQRTDDELARLAEI
                     RERIDRIATLDMAVVVSPGQNEIEQMKKLGLDIVPHRKRMVESDPPLDERFKDTQDPL
                     SLVFVCAMWLTGFDAPSCSTVYLDKPMRNHTLMQTIARANRVFPGKHSGLIVDYANVF
                     ASLEKALAIYGAGKGGQTPVRNKQQLLDDLRKAVADASAWCAARHVDLAAMEALPSGG
                     MDRLTQLVGAVDALISP"
BASE COUNT          236 a          294 c          351 g          172 t
ORIGIN      
        1 ctgatcgcgg gcgaggagcg caccaaggaa gtcttcggcg actacgtctc gatctacgac
       61 taccagcagt cggtcgagga cggggcgacc gtgccgctct tctacgaaaa tcgcacaccc
      121 gagctggaac tgaccaaccc cacgctcaac gaagacatct acgatattat cgaagaggcg
      181 gcgctcgacg aggcacagga agagcggttg gcgcaggtgc tcgggaagca ataccacatc
      241 atcacgcgcg acgaccggct cgacacggtg gcgcaggata tcgtgcgcca cttcctcgga
      301 cgcgggtttc aaggcaaggc gatagttgtc tcgatcgaca aagcgacggc gctgcggatg
      361 tatgacaagg tggagcgcga gctgatgatg gaagcgaaac gcttgaaggc ggaatggcga
      421 aagctcgagg acctgaaacg tcagcggacg gatgacgaac tcgcccggct tgcagagatc
      481 cgtgagcgca tcgatcgtat cgcaacgctg gatatggcgg tggtcgtatc accaggccag
      541 aacgagatcg agcagatgaa gaagctcgga ctcgacatcg tgccgcaccg gaagcgcatg
      601 gtggaatcgg atccgccgct cgacgaacgc ttcaaagaca cgcaggaccc cctctcgctc
      661 gtgttcgtgt gtgcgatgtg gcttacaggc ttcgacgcgc cgagctgttc gacggtgtat
      721 ttggacaagc cgatgcggaa ccacacgctg atgcagacga tcgcacgcgc gaatcgggtg
      781 ttcccaggga aacacagcgg tctgatcgtc gattacgcga acgtgtttgc gtcgctggaa
      841 aaagcgctcg cgatttacgg cgcggggaag ggcgggcaga cgccggtgcg caacaagcaa
      901 caattgctgg acgacctgag gaaagccgtg gcggacgcga gcgcgtggtg cgccgcccga
      961 cacgtcgacc ttgcggccat ggaagcgctg ccgagcggcg gtatggatcg gctgacgcag
     1021 cttgtcgggg ccgtcgatgc gttgatttct cca
//
LOCUS       DASPLT010000956         2682 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_51078, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000956 DASPLT010000000
VERSION     DASPLT010000956.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 2682)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2682)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2682
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_51078"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..394
                     /locus_tag="VEA63_09920"
     CDS             <1..394
                     /locus_tag="VEA63_09920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD84362.1"
                     /translation="DAATIAGWSAAETIQLGDPLDQTPGRVIALDISPMMQAVLGRTF
                     RQHGVLMKGFIEGVSVQALLDTSEAGLPGSFNGVRSESAGGRLGGQFTQVTSMQSPVS
                     DTNLVFVREGAAGVGVGLALLSVLGLWV"
     gene            405..962
                     /locus_tag="VEA63_09925"
     CDS             405..962
                     /locus_tag="VEA63_09925"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024967.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HYD84363.1"
                     /translation="MIDTSQTLQYAHLKKRDDVALLLPSGSIGIELGVAEGELASRLL
                     KAPMLRFLYGVDAYAGDRGHDIDQYKRVLRRLDCYRHRYSLLQMRFDQALDLFDDASF
                     DFIYVDGYAHTGEEGGATFREWWPKLKPGGVFAGDDYDPAWPDVVRHVDAFLADQQLT
                     GFVIPYVESETPYCKYPTWFTLKPA"
     gene            complement(965..1618)
                     /locus_tag="VEA63_09930"
     CDS             complement(965..1618)
                     /locus_tag="VEA63_09930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD84364.1"
                     /translation="MIALPLEDAPLDWAALATRRFPIGAKAYMQRREAVLEDHLEKLS
                     EEFAGESHLSYAHAILTVLIRRDLQTDLAFRHFEAMWSSQAEHLLANLSARWLISACD
                     TIMDCSDDEYERAMAAAGSLFFNTIKLYETERYHSDTTPAPKGDVGAPVPLFDGLTTF
                     SVGRGELISAQRERIKALARPDSTAASIVLELLNRADRNDTVFQRFAAQHHHDATRW"
     gene            2141..2314
                     /locus_tag="VEA63_09935"
     CDS             2141..2314
                     /locus_tag="VEA63_09935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD84365.1"
                     /translation="MFKPAHHFFVALNKSAKAARRGDVAAAERWQRVAERHLAIAERF
                     QKLAPDRNPWPGR"
     gene            2383..>2682
                     /locus_tag="VEA63_09940"
     CDS             2383..>2682
                     /locus_tag="VEA63_09940"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HYD84366.1"
                     /translation="MTSPLATIIVVTHNSARWLARMCAAVAAQTETRWRLVIIDNASR
                     ADERPHQTELPPGASIIQCERNLGFAEANNLAARDADTPYLVFLNPDAFPEPDWLS"
BASE COUNT          502 a          891 c          798 g          491 t
ORIGIN      
        1 cgacgccgca acgatcgccg gctggagcgc cgccgagacg atccagctcg gcgacccgct
       61 cgatcaaacg cccggccgcg tcatcgcgct cgacatctcg ccgatgatgc aggccgtgct
      121 cggccgcacc ttccgccagc acggcgtgct gatgaagggc ttcatcgaag gcgtcagcgt
      181 ccaagcgctg ctggacacca gcgaagccgg cctgcccggc tccttcaacg gcgtccgcag
      241 cgaaagcgcc ggtggccggc tcggcggcca gttcacacaa gtgaccagca tgcaatcgcc
      301 ggtgtcggac acgaatttgg tgtttgtgcg ggagggcgcg gcgggagtcg gagtgggtct
      361 cgcgctattg tcggtgcttg gtctatgggt gtagcacgcc gagaatgatc gatacctcgc
      421 aaacgttgca gtacgcgcat ctgaagaagc gcgacgatgt cgcgcttctt ctcccttccg
      481 gctcgatcgg catcgaactt ggcgtcgccg agggagaact cgcgtcacgt ttactgaaag
      541 cgccgatgtt gcgtttcctt tatggcgtcg atgcatacgc tggcgatcgc ggccacgata
      601 ttgatcagta caagcgcgtg ctgagacgac ttgactgcta ccgccatcgt tactcgctgc
      661 tgcaaatgcg tttcgatcag gcgctcgatc tgttcgacga cgcctcattc gacttcatct
      721 acgttgatgg atacgcgcac accggcgagg aaggcggtgc aacgtttcgg gagtggtggc
      781 ccaagctaaa gcccggcggt gtctttgccg gtgatgatta cgatcccgcc tggcccgatg
      841 tggttcgtca tgtggatgcg tttctcgcag atcaacagct cacaggcttc gtcattccct
      901 acgtcgagag cgaaacgccc tattgcaaat atccaacctg gttcacgctc aaacctgcgt
      961 gacgtcacca cctcgtggcg tcatgatgat gttgcgcggc aaagcgctga aaaaccgtgt
     1021 cattgcggtc ggcgcgattc aagagctcca ggacgatgct cgcggctgtg ctatcaggac
     1081 gagccagcgc cttgatgcgc tctctttgag ctgaaatgag ttcgccgcgt ccgaccgaga
     1141 atgtcgtgag gccgtcgaac agcggcactg gggcgcccac atcgcccttc ggggctggcg
     1201 tcgtgtcgct gtgatatcgc tctgtctcgt agagtttgat cgtattaaaa aacaacgacc
     1261 ctgcagccgc cattgcgcgc tcgtattcgt cgtctgagca gtccatgatc gtgtcgcagg
     1321 ccgaaatcag ccagcgtgcg ctcaaattcg cgagcaagtg ttccgcttgc gatgaccaca
     1381 tcgcttcgaa atgtcgaaaa gcgagatcgg tttgcaggtc gcggcgaatt aggacggtca
     1441 gaatggcgtg cgcgtagctc agatggctct cgcccgcgaa ttcttcactc aacttctcaa
     1501 ggtgatcctc aagcacggct tcccgccgct gcatgtaggc ctttgcgccg atggggaaac
     1561 gccgggtcgc aagcgccgcc cagtccagcg gcgcatcttc cagcggtagc gcgatcacgg
     1621 acgcgcgcac atcgcactcc caaccgacgc gcgctcgcgc aaaacgaatc tatccaacac
     1681 aatttccgaa aaaccacaac gtttccaaca ctccgctcaa aatcaatccg ccccctccaa
     1741 aaccgggtta gacttcttgc atcgctggtg ctggagggca gttgggaccc ctgccgaccg
     1801 acgcagcagc gagacggtcg agcgtgaagc cgatccggcg tgaaacccgg ctgaggccaa
     1861 gcccaagcga atgcggcgaa cgccgccaag cgcggggagt caccgggacc ttgcgtcggc
     1921 aagcgcaaga cccgcacgcc gtcggcgctc ttgccagagg cgtgaaacac cgccttgcgc
     1981 ggagccggcg tcggcaggcc gtcgggggcg cggaagatcc gcgtcttttc gcgccgttca
     2041 cgcggcgcat gctggcgtgg ttctcccgcg ccccctgccc tccgttttcg cgccgcaagg
     2101 ccgacggcgc ctccgcgcgc ctactgcaca aggagcgccc atgttcaaac ccgcgcatca
     2161 tttcttcgtc gcgctcaaca aaagcgccaa agcggcccgc cgcggcgatg tcgccgccgc
     2221 cgagcgctgg cagcgcgtcg ccgagcgtca cctcgccatc gccgagcgct tccagaagct
     2281 ggcgccggat cgcaatccgt ggccgggacg ctagccaccg cattgcgaaa atgtaggaac
     2341 gccgtcagtg tcgccgcctc tgcatttgtt gcccgagcgc gcgtgacctc ccctctcgcc
     2401 accatcatcg tcgtcacgca caacagcgcg cgctggctgg cgcgcatgtg cgcggctgtc
     2461 gctgcgcaaa ccgaaacgcg ctggcggctg gtgatcatcg acaacgcatc gcgcgcggat
     2521 gaacggccgc accaaaccga gctgccgccc ggcgccagca tcatccagtg cgaacgcaat
     2581 ctcggcttcg ccgaagccaa caatctcgcc gcccgcgacg cagacacgcc ctatctcgtc
     2641 ttcctcaatc cggacgcgtt ccccgaaccc gactggctca gc
//
LOCUS       DASPLT010000957         1191 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_12031, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000957 DASPLT010000000
VERSION     DASPLT010000957.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1191)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1191)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1191
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_12031"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..373
                     /locus_tag="VEA63_09945"
     CDS             <1..373
                     /locus_tag="VEA63_09945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD84367.1"
                     /translation="LCAVAGIAAPEGIDGESFLPALSGEAAPELERELYFVRREGRAR
                     FNYGGKTIEALVHRGWKLVHDNPFAAMELFHLERDPYETTDLREPQPEKFDDLSRRLR
                     RHIQRGGEVPWQRPLVRKTVE"
     gene            complement(404..>1191)
                     /locus_tag="VEA63_09950"
     CDS             complement(404..>1191)
                     /locus_tag="VEA63_09950"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HYD84368.1"
                     /translation="VVSVCMIFLTLRLGGTIARTGEWKWYALVAVVSLLCVLPATFLL
                     KEPNTPAPVAGKGRLRDYFDAVRNTPHFLRLCLVVATTFMGGQLITNYYRLFTKEQLG
                     LGLDEALRAFAWMPVIAFFASYPIGWLADRISPKYVTQAGAVLLALSGFCGVRADSIW
                     DLRLMALLVGMGTLCVEVACNAYLFSFLPPGRNGQLSGFVNIFRGGPRFLMFFGAGLF
                     IEIFDRNYRIAFGGSIVCAIVAFILLCGLPRQGNPSRESGSTT"
BASE COUNT          262 a          315 c          435 g          179 t
ORIGIN      
        1 gctctgtgcg gttgcgggta tcgcggcgcc ggagggaatc gatggagaaa gttttctgcc
       61 ggcactgtcg ggcgaagctg cgccggagct cgagcgggag ttgtatttcg tgcgtcgcga
      121 ggggcgcgcg cgcttcaatt acggaggaaa gacaatcgag gcgctggtgc atcgcggttg
      181 gaaactggtt cacgacaatc cgttcgcggc gatggagctg tttcatctcg agcgcgatcc
      241 ttatgagacg accgatctgc gcgagccgca gccggagaag ttcgacgacc tgtcgcggcg
      301 gttaaggcgg cacattcagc gcggcggcga agtgccgtgg cagcgaccgc tggttcggaa
      361 aacggtggag tgacgtggcg gacgttgcgc gcgagcgcgc aacctacgtg gttgagccag
      421 attcacggga ggggtttccc tggcggggca ggccgcacag gagaatgaag gcgacgatcg
      481 cgcagacgat ggaaccgccg aaagcgatgc ggtagttgcg gtcgaaaatc tcgatgaaca
      541 aaccggcgcc gaaaaacatc aggaagcgag gaccgccgcg aaagatgttc acgaagcccg
      601 agagctggcc gttgcgtccg ggcggaagaa aactgaaaag gtaggcattg caggcgacct
      661 cgacgcagag cgtgcccatt ccaacgagca gggccatgag gcggagatcc caaatcgagt
      721 cggcgcgcac accgcagaaa ccggacaaag cgagcaggac ggcgccggcc tgcgtcacgt
      781 atttcgggct gatgcgatcg gcgagccagc cgatgggata gctggcgaag aacgcgatga
      841 cgggcatcca ggcgaaggcg cgcagcgcct cgtcgagccc gagtccgagc tgttccttgg
      901 tgaagaggcg atagtagttc gtgatgagtt ggccgcccat gaacgtggtc gcgacgacga
      961 ggcagagccg gagaaaatgt ggcgtattgc gcacggcatc gaagtagtca cgcaggcggc
     1021 ccttcccggc gacgggcgcc ggcgtgttgg gttccttgag gagaaacgtg gcgggcagca
     1081 cgcaaagcag ggagacgacg gcgacgagcg cataccattt ccattcgccg gtgcgggcga
     1141 tggtgccgcc gaggcgaagc gtgagaaaaa tcatgcaaac cgaaacgacc t
//
LOCUS       DASPLT010000958         2407 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_71624, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000958 DASPLT010000000
VERSION     DASPLT010000958.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 2407)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2407)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2407
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_71624"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            64..813
                     /gene="lpxA"
                     /locus_tag="VEA63_09955"
     CDS             64..813
                     /gene="lpxA"
                     /locus_tag="VEA63_09955"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012377339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HYD84369.1"
                     /translation="MIHPTAIVEEGAQLGAGCEIHAHAIVTKHGVLGENVIVHPFAVV
                     GGDPQYLKFDRATASGVRIGSGTVIREHVTVNRSIHGGGYTIVGENCFLMAASHVAHD
                     CVVGNQVVLANAALLAGHVTVGDHAFLGGNCAVHQFCRIGEGVMVAGLAAITRDVAHF
                     TMVAERDDVVGFNAIGLKRRGIGRAAIAELKQAFHTVYFTPGNIRVIAAEALASGAYG
                     TAEAKRFLEFFSEGKRSFARARRAAAGDAEG"
     gene            837..1787
                     /gene="pdxA"
                     /locus_tag="VEA63_09960"
     CDS             837..1787
                     /gene="pdxA"
                     /locus_tag="VEA63_09960"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009508587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HYD84370.1"
                     /translation="MKLAITCGDPAGVGPEIVAAWLAAHPEQAADVAAIGPACWLATL
                     DARATKIAVGPENFLATPGRPTEAGAEVAWAALERAAAGCAQGEFAGVVTGPVSKEWL
                     ARVGYAFPGQTEFFAARWGGEPVMAFCGGKLRVVLATWHVPLHEVARLLGPNLLRRTI
                     AAASELVRAELANGARRGAETPPYIPRIGVCGLNPHAGEGGLIGYEERDLLNPALDHL
                     RREFPGVSRCEPGDTLFARALRGEFDVVVALYHDQGLAPLKVIDFDEAVNVTLGLPHV
                     RTSPDHGTAFGIAGKGVARTTSFANAVTVARRLINWRAAR"
     gene            1796..2227
                     /locus_tag="VEA63_09965"
     CDS             1796..2227
                     /locus_tag="VEA63_09965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009508584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly accessory protein"
                     /protein_id="HYD84371.1"
                     /translation="MSDSATLSPANTPTAPSGAAMPAAGALPEGVREGNENLIKLTAA
                     AGAKVNSLIAREGQGRFLRIAISGGGCNGLSYKMKFVAEPKRGDILVRTGGVEVVVDS
                     KSALYLKGTHLDYSSAMVAGGFKFSNPNAKASCSCGESFSV"
BASE COUNT          396 a          600 c          939 g          472 t
ORIGIN      
        1 gagaggagcc tgaacattga gaattctctg gagcttgggg cttggagctt ggagctttct
       61 ttcgtgattc atcccacggc gattgttgaa gaaggcgcgc agctcggtgc gggttgcgag
      121 atccatgcgc acgcgatcgt gaccaagcac ggtgtgctcg gcgaaaatgt gatcgtgcat
      181 ccgtttgcgg tggtcggggg cgatccgcag tatttgaagt tcgatcgggc gacggcgtcg
      241 ggtgtgcgca tcggatcagg caccgtgatt cgcgagcacg tgacggtgaa ccgttcgatt
      301 cacggcggcg gttacacaat cgtcggggag aattgttttt tgatggcggc gagtcatgtg
      361 gcgcacgatt gcgtggtggg gaatcaggtc gtgctcgcga atgcggcttt gctggcgggg
      421 cacgtcactg tcggggacca tgcgtttctc ggcggcaact gcgcggtgca ccaattttgt
      481 cggatcggcg agggagtaat ggtcgcgggc ttggcggcga tcacgcgcga cgtggcgcat
      541 ttcacgatgg tggcggagcg cgatgatgtc gtcggtttca acgcgatcgg tttgaagcgt
      601 cgcgggatcg gtcgcgcggc gatcgcggaa ttgaagcagg cgtttcacac ggtgtatttc
      661 acgccgggaa acattcgcgt gatcgcggcg gaggcgctgg cgtcgggcgc ttacggaacg
      721 gcggaagcga agcggtttct cgagtttttc tccgaaggga agcggagttt cgcgcgcgcg
      781 cggcgggcgg cggcaggtga tgcggagggt tgagcgagaa gcggacgaag gcgtttatga
      841 aactagcgat cacgtgcgga gatccggcgg gggtgggacc ggagattgtc gcggcgtggc
      901 tcgcggcgca tccggagcaa gcggcggatg tcgcggcgat cggcccggcg tgctggctgg
      961 cgacgctcga tgcgcgagcg acgaaaatcg cggtgggtcc ggagaatttt ttggcgacgc
     1021 caggacggcc gacggaagcg ggtgcggagg tggcgtgggc ggcgctcgag cgcgcggcgg
     1081 cgggctgcgc gcagggggag tttgcaggcg tggtgacggg gccggtgagc aaggagtggc
     1141 tcgcgcgcgt cggttatgcg tttcccggac agacggagtt tttcgcggcg cgttggggag
     1201 gtgagccggt gatggcgttt tgcggcggaa agctgcgcgt ggtgctcgcg acctggcatg
     1261 tgccgctaca cgaagtggcg cggttgctcg ggccgaattt gctgcggcgg acgatcgcgg
     1321 cggcgagcga gctggtgcgg gcggagttgg cgaatggcgc gcggcggggt gcggagaccc
     1381 cgccctacat tccgcggatc ggggtgtgcg ggctgaatcc gcatgcgggc gaaggcgggt
     1441 tgatcggtta tgaagagcgg gatttgttga atccggcgct cgatcattta cgccgcgagt
     1501 ttccgggcgt gtcgcgttgc gaaccgggtg acacgctgtt cgcgcgcgcg ttgcgcggcg
     1561 agttcgacgt cgttgtggca ctttatcacg atcaaggatt ggcgccgttg aaggtgatcg
     1621 atttcgatga agcggtgaac gtcacgcttg ggctgccgca cgtgcggacg agtccggatc
     1681 acggcacggc gtttgggatc gcgggaaagg gcgtggcgcg aacgacgagc tttgcgaacg
     1741 cggtgacagt ggcgcggcgc ttgattaact ggcgggcggc gcgatagtcg gcgtgatgtc
     1801 agattccgcg acgctttctc ctgcgaacac gccgacagcg ccttcgggcg ccgcgatgcc
     1861 ggcggccggc gcattgccgg agggggtgcg cgaaggaaac gagaacctga tcaagttgac
     1921 ggcggcggcg ggcgcgaagg tgaactcgct gatcgcgcgc gaagggcagg ggcgttttct
     1981 gcggatcgcg atctcgggtg gcggatgcaa cggtttgagc tacaagatga agttcgtggc
     2041 ggagccgaag cgcggcgaca ttcttgtgcg gacgggcggg gtggaggtgg ttgtcgatag
     2101 caagagcgcg ctctatctga aagggacgca tctggactat tcgtcggcga tggtcgcggg
     2161 cgggttcaaa ttctcgaatc cgaatgcgaa agcgagttgc tcctgtggag aaagctttag
     2221 cgtatgagtt cgcgctcgga cctcatcatc ggggtcgccc cgactaaata gatgttgtca
     2281 acgtctcgca tccgggcgaa aacccgcgcg attcagcact gatcacccaa cctaattgat
     2341 ggccaattcg tttccttccg ccgggggcgg ctcccggcca ggctcttatc agcgtcgtaa
     2401 cgtcgac
//
LOCUS       DASPLT010000959         1682 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_215489, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000959 DASPLT010000000
VERSION     DASPLT010000959.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1682)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1682)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1682
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_215489"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1027
                     /locus_tag="VEA63_09970"
     CDS             <1..1027
                     /locus_tag="VEA63_09970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD84372.1"
                     /translation="NAARRVEALAVGRWTAISGAAFTTGTGATTSLGLSLLLGLANVR
                     LGYWWNSGVLPSDRETTTRPNFLELGSRILSRILPVQSSLLTECFARFHGPGRQHWYL
                     SDGGHFENSACYELIRRRVPFIICSDAGQDPEYQFEDFANLVRKARTDFGAEIQVVRR
                     TADLATDDPGARFPMPKLEEIVHPALADVIGVPEDFLPLERDEDEEVAEQQLPRACCS
                     RRHAILARIHYTDNDEFSWLLLVKPCVMGDETADVLQYQRSHRLFPQEPTTDQYFDEA
                     QWESYRKLGEHIGTELFTPPAVSDAKGANWFPAMMRPPEPTRFNFDVFDSARPTIAPP
                     ETPRIVR"
     gene            complement(1131..>1682)
                     /locus_tag="VEA63_09975"
     CDS             complement(1131..>1682)
                     /locus_tag="VEA63_09975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007360842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HYD84373.1"
                     /translation="EKNPHVTFFFNNYRKEPFPGEDRACPASPKVPTYDLQPEMSASE
                     VTREAKAAILSGKYGLVVVNYANPDMVGHTGSLPAATKAVEATDRGVGELLDALARMK
                     GRAVICADHGNAEQMWDPTVNGPHTAHTLNLVEVFVVGEGFAAGKTKMREGGRLADIA
                     PTVLHLMGLPKPAEMTGESLVVG"
BASE COUNT          307 a          597 c          466 g          312 t
ORIGIN      
        1 caacgccgcc cggcgggtcg aagccctcgc cgtcggccgc tggactgcga tttccggcgc
       61 cgccttcacc accggaacgg gcgccaccac ctccctcggg ctcagcctgc tgcttggcct
      121 cgccaacgtg cgcctcggtt actggtggaa cagcggcgtg ctcccgagcg atcgcgaaac
      181 cacaacgcga cccaatttcc tcgaactcgg cagccggatc ttgagccgga tactccctgt
      241 ccaaagcagc ctgctcacgg agtgctttgc gcgcttccat ggcccgggcc gccagcattg
      301 gtatctctcg gacggcgggc atttcgaaaa cagcgcgtgc tacgagctga ttcgccgccg
      361 ggtgccgttc atcatctgca gcgacgccgg acaagacccg gagtatcagt tcgaggattt
      421 cgcgaatctc gttcgtaaag cgcgcacgga ttttggcgcc gagattcaag tcgttcgccg
      481 caccgccgac cttgcgaccg acgatccggg cgcgcgcttt cccatgccga agctcgaaga
      541 gatcgttcac cccgcgctgg ccgatgtcat cggcgtgccc gaggactttc ttccacttga
      601 acgcgacgag gacgaagaag tcgccgaaca gcaactgccg cgggcctgct gctctcgacg
      661 tcatgcgatc ctcgcgcgta tccactacac ggataacgac gagttctcct ggctgctgct
      721 cgtcaaacct tgcgtcatgg gcgatgagac cgccgatgtg ctacagtatc agcgcagcca
      781 ccggctcttc ccgcaggaac cgacgaccga ccaatacttc gacgaagccc aatgggagag
      841 ctaccgcaaa ctgggcgaac acatcggcac cgaactcttc acacctcctg cggtgagcga
      901 tgcaaaaggg gccaattggt ttccggccat gatgcgcccg cctgaaccta ctcggttcaa
      961 tttcgacgtc ttcgactctg cgcgccccac gatcgcgccg cccgaaactc cccgcatcgt
     1021 tcgctgacac aactacgcgt cagcccatcg aaacccggta atgggcgacc ttactggagc
     1081 tgaacaaaat tgtggccgcg accctcgtgt gagcgcggcc accgcctctc tcacccgacc
     1141 accagactct cgcccgtcat ctccgccggt tttggcaggc ccatcaagtg cagcaccgtc
     1201 ggcgcgatgt cggcgagacg tccgccttcg cgcatcttcg tcttgcccgc ggcaaacccc
     1261 tcgccgacga cgaacacctc cacaagattc aaggtgtgcg cggtgtgcgg accgttgacc
     1321 gtcggatccc acatctgctc cgcattcccg tggtcggcgc aaatcaccgc acggcccttc
     1381 attcgcgcga gcgcgtcgag cagttcgccg acgccgcggt ccgttgcctc gaccgccttc
     1441 gtcgccgccg gcaacgagcc ggtgtggccc accatgtcgg gattcgcgta gttgacgacc
     1501 acgagcccat atttccccga aaggattgcc gccttcgctt cacgcgtcac ctcgctcgcc
     1561 gacatctcgg gctgcaggtc atacgtcggc accttcgggc tcgcgggaca tgcgcggtct
     1621 tcacccggaa aaggctcctt gcgatagttg ttaaagaaga acgtcacatg aggattcttc
     1681 tc
//
LOCUS       DASPLT010000960         1355 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_51131, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000960 DASPLT010000000
VERSION     DASPLT010000960.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1355)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1355)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1355
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_51131"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(39..1130)
                     /locus_tag="VEA63_09980"
     CDS             complement(39..1130)
                     /locus_tag="VEA63_09980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD84374.1"
                     /translation="MDSTPLYHPPSPKKPSFPVRSGLRGYLRRYRRDRELPVTYERLL
                     GFHEAVPLNDAHGKPTLWDSVIYHAEEMTELYEGLKRIYALLRVDGDLTVMKHLYVDR
                     VDYCSFGNSTPFRIRVVNAFNDNQDYFYVKKADASRVYGLELEHLLSPNRINYFTCDN
                     TLIEEHIVGIPGDVFIAQHLNAGQLKPIRIAKELVKFNERCFVRLLGDMRSYNFVAVI
                     TPDFEEAQLRIRAMDFDQQSYSGRKNFYLPQFFLDNAPLAQFCISHLNPQTAWQYQRE
                     EHAVMLQRAGIIADRLVYLLEEMSRDPIAPPEKVHELRASLAEYFQRNSYLRCESMGA
                     LVRENLESIRRRIGPAAPPALVTLAAKRE"
BASE COUNT          266 a          453 c          381 g          255 t
ORIGIN      
        1 ctggagacac ggcgcccccg ccgcgcattc accgcggact actcgcgctt cgccgccagc
       61 gtcaccaacg ccggcggcgc cgccggcccg atccgccgtc gaattgactc caagttttcg
      121 cgaaccagcg cgcccatcga ttcgcaccgt agatagctgt tgcgctgaaa atattccgcg
      181 agactcgcgc gcagctcgtg cactttttcc ggcggcgcga tcgggtcgcg tgacatctct
      241 tcgagcaaat acaccagccg gtccgcaatg atgcccgcgc gctgcaacat caccgcgtgc
      301 tcctcgcgct gatactgcca cgcagtctgc ggattcaagt ggctgatgca aaactgggcg
      361 aggggggcgt tgtccaagaa aaactgcggc agataaaaat tcttccgccc gctgtaggac
      421 tgctggtcga aatccatcgc gcgaattcgc aactgcgcct cctcgaaatc cggcgtgatc
      481 accgccacga agttgtagga ccgcatgtcg ccgagcagac gcacgaagca gcgctcgttg
      541 aacttcacca actccttcgc gatccggatc ggcttgagct gtccggcgtt caaatgctgc
      601 gcgatgaaca cgtcgccagg aattccgacg atgtgctcct cgatcagcgt gttgtcgcac
      661 gtgaaatagt tgattcggtt aggcgacagc aagtgctcga gctcgagccc gtaaacacgc
      721 gaggcgtccg ccttcttcac gtagaagtaa tcctggttgt cgttgaacgc attcacgacg
      781 cggatacgga acggcgtcga gttgccaaag ctgcagtagt ccacgcggtc gacatacagg
      841 tgcttcatca ccgtcagatc accgtcgacg cgcagcagtg cgtagatccg cttcagccct
      901 tcgtagagct ccgtcatttc ctccgcgtgg taaatgacgc tgtcccacaa cgtcggtttg
      961 ccgtgcgcat cgttcaacgg caccgcttcg tgaaatccca gcagtcgctc gtaggtgacc
     1021 ggcaactcgc gatcgcgccg gtagcgtcgc agatatccgc gcagcccgct gcgcaccggg
     1081 aagctcggct tcttcggcga cggcggatga tacagcggag tcgaatccac gggcacaacg
     1141 tagcccgtcg cgtctcacgc gcgagcccgg tttgacgctt ccggtcacga cgcggcgcgc
     1201 cgcctcaacg tgccaccggc acctcgaacc aaaatacgct gccgccactc gcgggaaaac
     1261 tggcacctac gttcccgccc tgcgactcga tcaagtgttt tacgatggaa agccccatgc
     1321 catgactctc ctcgccgccg gtggtccgga ccgac
//
LOCUS       DASPLT010000961         1515 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_35090, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000961 DASPLT010000000
VERSION     DASPLT010000961.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1515)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1515)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1515
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_35090"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..224)
                     /locus_tag="VEA63_09985"
     CDS             complement(<1..224)
                     /locus_tag="VEA63_09985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009513877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HYD84375.1"
                     /translation="MNRTDRLVAMVMFLQGRRVVKADELAAHFEVNVRTIYRDVSALC
                     EAGVPVIGEAGVGYSLVKGYHLPPVMFTAE"
     gene            complement(354..848)
                     /locus_tag="VEA63_09990"
     CDS             complement(354..848)
                     /locus_tag="VEA63_09990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012372899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HYD84376.1"
                     /translation="MSRVVTFHYTLRDPAGRVLDTSAGGPAISYLEGAGQIIDGLDEQ
                     LRAAAAGEKLRVEVPAAKAYGERDTAQVQRVNRALLPVEGELKIGDTFQAGEDRHAPV
                     VTVMGIEGDDVLLDANHPLAGVDLTFEVEVVAARAATAEELRHGHAHGGVEGGGCGDG
                     CGCA"
     gene            complement(1205..>1515)
                     /locus_tag="VEA63_09995"
     CDS             complement(1205..>1515)
                     /locus_tag="VEA63_09995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD84377.1"
                     /translation="LEFSGTKGRVAVNFFADDPVRLETVRGIETFSLPNPPHVAQPLI
                     QTVVDELLGRGTCPSTGESARRTQAVMDRVLSEYYSGREDAFWLRSETWPGRKRIRNA
                     "
BASE COUNT          309 a          618 c          345 g          243 t
ORIGIN      
        1 tcctccgccg tgaacatcac cggcggcaaa tgatacccct tcaccaaact gtaaccgacg
       61 cccgcctcgc caatcaccgg cacacccgcc tcgcacaacg ccgacacgtc gcgataaatc
      121 gtccgcacat tcacctcgaa gtgcgccgcg agctcgtccg ccttcacgac gcgtctccct
      181 tggagaaaca tcaccatcgc gacgagtcgg tccgttcgat tcatgccgcg agactgcgac
      241 agccaccccg ccgacgccaa acggtttctc cctaaaaatc gcgccgccgc cacgggtggc
      301 ccgccgcgtg agcggcggga ttccgcgctc acgcccacct ctccctcgcc acctcacgcg
      361 cacccgcacc catcgccaca tccgccgccc tccactcccc cgtgcgcatg cccatgacgc
      421 aactcctccg ccgtcgccgc ccgtgccgca acgacctcca cctcgaacgt caaatccacg
      481 cccgccagcg gatgatttgc atcgagcaaa acgtcgtcgc cctcgatgcc catgacggtg
      541 acgaccggcg catgccgatc ctcgcccgcc tgaaacgtat cgccaatctt caactcgcct
      601 tcaacgggca gcagcgcgcg attcacgcgc tgcacctgcg ccgtatcgcg ctctccgtaa
      661 gccttcgccg ccggcacctc gacgcgcagc ttctcgcccg ccgccgccgc tcgcagttgc
      721 tcatcgagtc catcgatgat ctgccccgcg ccttccaaat aggaaatcgc cggcccgcca
      781 gccgaagtat cgagcacgcg acccgccgga tcacgcaaag tgtagtgaaa cgtaacaaca
      841 cgagacatcc aaaaagtaac gcgtcaccct gccgaactca caaaccgctt ttgctctctc
      901 cgtagccgca ggtctccata cctgccaacc tccccatttt caggaaaggt aggtggtgcg
      961 cttgcgcacc acgtcgcgcg cgagcgcgca acctaccctc ctcttcgtag cggcaggcct
     1021 ccgggcctgc cagcccgcca tcgctcattt ccacgaaaca aactccgccg aacgccgaaa
     1081 aaaaacgtcc gcgctccgcc gatcaggcga agcacgcact catccgccct caccaccggc
     1141 cctcggaaaa tcagcgcaaa tgagcggtta aagaaactcc gtcctcctcc gccaagccgc
     1201 tcgctcaagc attccgtatc cgcttccgcc ccggccaagt ttccgaccgc aaccaaaacg
     1261 catcctcgcg cccgctgtaa tactcgctca gcacccgatc catcaccgcc tgcgtccgcc
     1321 gcgccgactc gccggtgctg ggacacgttc cccgccccaa cagttcgtcg accaccgtct
     1381 gtatcagcgg ttgtgctaca tgcggcggat tcggcagcga aaacgtttcg atccctcgca
     1441 ccgtttccaa acgcaccgga tcatccgcaa aaaaattcac cgccacgcga cccttggtcc
     1501 cggaaaattc cagca
//
LOCUS       DASPLT010000962         1039 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_51149, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000962 DASPLT010000000
VERSION     DASPLT010000962.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1039)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1039)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1039
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_51149"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1039
                     /locus_tag="VEA63_10000"
     CDS             <1..>1039
                     /locus_tag="VEA63_10000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009511546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HYD84378.1"
                     /translation="KNQKSKIESADEALAGARDIIAERVSDDKDARARLRTRYQSDAI
                     ISSKVLIGKDTAPDAAKYKDYFEWSEPLAKAPSHRVLAMRRGEKETFLMMRITLPDET
                     LAIAEVEPLFVKGNKSDKVRAAIPAVLSSTTTVPSTSAPIPGRLGTSAPTSCSEHVRL
                     AVTDACKRLLCPAMETEMRLESKKRADEAAIKVFSDNARELLLAPPLGQKAVMAVDPG
                     FRTGCKVVLLDRQGKLLHNDVVYPDRHEDEAKAKLAGFVQFFNVEAIAIGNGTAGRET
                     EAFIRKIGLPATIPIVMVNESGASIYSASEVAREEFPDHDLTVRGAVSIGRRLMDPLA
                     ELVKLDPKSIGV"
BASE COUNT          224 a          361 c          284 g          170 t
ORIGIN      
        1 aagaaccaga aatccaaaat cgaatccgcc gacgaggcgc tcgccggcgc ccgcgacatc
       61 atcgcggagc gggtcagcga tgacaaagac gcgcgcgccc gtttgcgcac gcgttaccag
      121 tccgacgcga tcatttcctc caaggtgctg atcggcaaag acaccgcccc cgacgccgca
      181 aaatacaaag actatttcga atggagcgaa ccgctcgcga aagctccttc gcaccgcgtc
      241 ctagccatgc gccgcggcga aaaggaaacg ttcctcatga tgcgcatcac cttgcccgac
      301 gagaccctcg ccatcgcgga agtggagccg ctgttcgtga agggaaacaa atctgacaag
      361 gtgcgggcgg ctatcccagc cgtcctgagt tcgacgacca ccgtcccatc gacttcagcg
      421 cccattccag gacgcttggg gacaagcgct cctacctcct gcagcgaaca cgtccgcctc
      481 gccgtcaccg acgcctgcaa gcgcctgctc tgccccgcga tggaaacgga aatgcgtctc
      541 gaatcgaaga agcgcgccga cgaagccgcg atcaaagtct tttccgacaa cgcacgcgag
      601 ctcctgctcg ccccgcctct cggccaaaaa gcggtgatgg ccgttgatcc cggtttccgc
      661 accggctgca aagtcgttct tctcgatcgc cagggaaaac tcctgcacaa cgacgtcgtc
      721 tatcccgacc gccacgaaga cgaggccaag gcaaaactcg ccggcttcgt gcagttcttc
      781 aacgtcgagg ccattgccat cggcaacggc accgcggggc gcgagaccga ggccttcatt
      841 cgcaagatcg gcctgcctgc cacgattccg atcgtgatgg tcaatgagtc cggcgcgtcg
      901 atctactccg ccagcgaggt cgcccgcgaa gagtttcccg atcacgatct caccgtccgc
      961 ggcgcggtat ccattggacg gagactcatg gatcccctcg ccgagctcgt gaagctcgat
     1021 ccgaaatcca tcggcgtcg
//
LOCUS       DASPLT010000963         1007 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_154825, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000963 DASPLT010000000
VERSION     DASPLT010000963.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1007)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1007)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1007
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_154825"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..986)
                     /locus_tag="VEA63_10005"
     CDS             complement(<1..986)
                     /locus_tag="VEA63_10005"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HYD84379.1"
                     /translation="MAVVIVAAALLIGRLLRSMVIARLESAHAAVRRIGDTSDLSVRL
                     PAEGSDELSGLSAGINRMLEALARGEAQRVAAEQERERLRAQLQHVQKMEAIGTLAGG
                     LAHDFNNLLTSIQGSSTLIRLEGQPSAINERHLRRIEQATSNAVALVRQMMAFGRRNP
                     TVFAHHHLGKIVQDALQLLRSSIPRTIEFEVIGDEADDVVHGDAGQLQQVLVNLGTNA
                     SQAMAGGRGRFTIAIARVRLPDPARAETANLSGEYLRLSVSDTGCGIEPENLQRVFEP
                     FYTTKPVGSGTGLGLAVVHGIISHHHGTIAVESEVGRGTTFVVHLPKADSAT"
BASE COUNT          188 a          373 c          275 g          171 t
ORIGIN      
        1 tccgtcgcgg aatccgcttt cggcaagtgg acgacaaacg tcgtgccgcg accgacctcg
       61 ctttcgaccg cgatcgttcc gtgatgatgc gaaatgatcc cgtgcaccac ggcgaggccc
      121 aggcccgtgc ccgaaccgac cggcttcgtc gtgtaaaacg gctcgaagac acgctgcagg
      181 ttttccggct caatgccgca accggtgtcg ctgaccgaca gcctcagata ctcgcccgaa
      241 agattcgccg tttccgctct tgccggatcg ggcaaacgca cacgcgcgat cgcaatcgtg
      301 aaccgccccc gtccgcccgc catcgcctgg ctggcgttgg ttcccaggtt caccaacacc
      361 tgctgcagtt ggccggcatc gccgtgaacc acatcgtccg cctcgtcgcc gatcacctcg
      421 aattcgatcg tgcgcgggat gctcgaccgc agcaattgca gcgcatcctg cacaattttg
      481 ccgagatgat gatgcgcgaa cacggtcgga ttccgccggc caaacgccat catctgccgc
      541 accaacgcca ccgcattcga cgtcgcctgc tcgatccgcc gcaaatgccg ctcgttgatc
      601 gccgaaggct gcccctcgag ccggatcagc gtagaagaac cctggatact cgtgagcagg
      661 ttgttgaaat cgtgcgccag tccgcccgcg agcgtgccga tcgcttccat tttctgcacg
      721 tgctggagct gcgcccgcag ccgctcgcgc tcctgctcgg cggcgacgcg ttgcgcttcg
      781 ccgcgggcca gcgcctcgag cattcggttg attcccgcac tcaagcccga cagctcgtcc
      841 gagccttccg cgggcaaacg caccgaaaga tccgacgtgt cgccgatccg acgcaccgcc
      901 gcatgcgcgg actccagccg cgcgatcacc atcgaccgca acaaccgccc gatcagcaac
      961 gcggccgcca cgatcacgac cgccatcacg acataaaaaa tcatccg
//
LOCUS       DASPLT010000964         2224 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_35138, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000964 DASPLT010000000
VERSION     DASPLT010000964.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 2224)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2224)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2224
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_35138"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..411
                     /locus_tag="VEA63_10010"
     CDS             <1..411
                     /locus_tag="VEA63_10010"
                     /inference="COORDINATES: protein motif:HMM:TIGR02603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD84380.1"
                     /translation="RWAYSSASGRRTFQNLCAACHVYENEGGKLGPDLTGSWRNGADY
                     FIENIVDPNAVVGTDFQLNLVTKRDGSVVSGMIERETDTALVVRTVTETINVPKDQVK
                     SRQVLEQSLMPPGLLDSLSERQVIELLMFLTESR"
     gene            432..>2224
                     /locus_tag="VEA63_10015"
     CDS             432..>2224
                     /locus_tag="VEA63_10015"
                     /inference="COORDINATES: protein motif:HMM:TIGR02604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PVC-type heme-binding CxxCH protein"
                     /protein_id="HYD84381.1"
                     /translation="MTSRVFSLALALLAAVSVLDAQEGFPVAAGSGLAPEAAARAMSL
                     PTGFRATLFAGEPDVHQPIAFTIDERGRLWVVENYSYPDWSPYGRDRVVIFEDEDGDG
                     HFDTSKVFYDQLNFASGIAVGHGGAWIGSPPYLLFIPDKNRDDIPDGPPQPVLDGWGH
                     HDTHETLNSFVWGPDGWLYGNQGVFTHSNVGAPGARDSARVPLNACVWRYHPTKKIFE
                     VFAEGMSNQWGLDFNDVGDAFVTACVIPHLYHVIQGGAYQRQSGPHANPHVYADIKTI
                     GDHVHYDRGKSWVEARLGQGGTDAAGGGHAHAGALVYLGDNFPAEYRGSLLMHNVLGS
                     RINRDVLEPAGSGYIGRHAPDFMKANDAWFRGLRLELGPDGSIFNSDWYDPRACHQQR
                     PHDRTNGRIYKISYGDPKPVRVDLKKFSSPELVALQLHANEWFVRRSRLLLQERGPDP
                     VVHTALIKLIREQTDVTRRLRALWALHVTRGLDEALALELLNDAEPYLRGWTIQLACE
                     DKAPSAALLARFTELARNEPSPVVRRFLASSAQRIPVAKRWDIVEALLARGEDANDQN
                     LPFLYWFAAEPLVPADPARALALAGQTPLTAV"
BASE COUNT          385 a          850 c          591 g          398 t
ORIGIN      
        1 cgttgggcct acagcagcgc gtcgggccgc cgcacgtttc aaaacctctg cgccgcgtgt
       61 cacgtttacg aaaacgaagg cggaaaactc ggtcccgatc tcaccggctc gtggcgcaat
      121 ggcgccgatt attttatcga aaacatcgtc gatcccaacg ccgtcgtcgg caccgatttt
      181 caactcaacc tcgtcaccaa acgcgacggc tccgtcgtct ccggcatgat cgaacgcgag
      241 accgacaccg cgctcgtcgt gcgcaccgtc accgaaacga tcaacgtccc gaaggatcag
      301 gtgaagagcc gccaggtgct cgaacaatcg ctcatgcctc caggcctcct cgactcgctc
      361 tccgaacgtc aggtcatcga gttgctcatg tttttaaccg aaagccgatg acgttgtctt
      421 tgtcctcttc catgacctcc cgcgtttttt ccctcgccct cgcgctcctc gctgccgttt
      481 ccgtgctgga cgcccaggag ggatttcccg tcgccgccgg ctccgggctg gctcccgaag
      541 ccgccgccag ggcgatgagt cttccaaccg gcttccgcgc cacgctcttc gccggcgagc
      601 ccgacgtcca tcagccgatc gccttcacca tcgacgaacg cgggcgcctg tgggtcgtcg
      661 aaaactactc gtatcccgac tggtcgcctt acggccgcga tcgcgtcgtc attttcgaag
      721 acgaggacgg cgacgggcat ttcgacacga gcaaggtttt ctacgaccag ctcaacttcg
      781 ccagcggcat cgccgtcggc cacggcggcg cctggatcgg cagtccgccc tatctgctct
      841 tcattcccga caagaatcgc gacgacatcc ccgacggccc gccgcaaccc gtcctcgacg
      901 gctggggcca ccacgacacc cacgaaacgc tcaacagctt cgtgtggggt cccgacggct
      961 ggctctacgg caaccagggc gtttttaccc actccaacgt cggcgcgccc ggcgcccgtg
     1021 actccgcgcg cgtgccgctc aacgcgtgcg tctggcgcta tcaccccacc aaaaagatct
     1081 tcgaggtctt cgccgagggc atgagcaatc agtggggcct cgacttcaac gacgtcggcg
     1141 acgcgttcgt caccgcgtgc gtgattccac acctttatca cgtcattcaa ggcggcgcct
     1201 atcaacgtca gtccggtcca cacgccaacc cgcacgttta cgccgacatc aaaaccatcg
     1261 gcgatcacgt ccattacgat cgcggcaaga gctgggtcga ggcgcgtctc ggccagggcg
     1321 gcaccgatgc cgccggcggc ggtcacgcgc acgccggcgc gttggtttac ctcggcgaca
     1381 atttccccgc cgaataccgc ggttcactcc tgatgcacaa tgtcctcggc agccgcatca
     1441 atcgcgacgt tctcgagccc gccggctccg gctacatcgg ccgccacgcg ccggatttca
     1501 tgaaggccaa cgacgcgtgg ttccgcggtc tccgcctcga gctcggcccc gacggctcga
     1561 tcttcaacag cgattggtat gacccgcgcg cctgccatca gcagcgtccg cacgatcgca
     1621 ccaacggccg catctacaaa atctcctacg gcgatccgaa gcccgttcgc gtcgatctca
     1681 aaaaattctc cagccccgaa ctcgtcgcgc ttcaactcca cgccaacgaa tggttcgtcc
     1741 gccgctcgcg tcttctgctc caggagcgcg gccccgatcc cgtcgttcac accgcgctca
     1801 tcaagctcat ccgcgagcag accgacgtca cccgccgcct tcgcgcgctc tgggcgttgc
     1861 acgtcacgcg cggcctcgac gaagcgctcg cgctcgaatt gctaaacgac gccgagccct
     1921 accttcgcgg ttggacgatt cagctcgcct gcgaagacaa agcgccgtcc gcggccttgc
     1981 tcgcccgttt caccgaactc gcgcgcaacg agccgtcgcc cgtcgtccgc cgctttctcg
     2041 cctccagcgc gcaacgcatt cccgtcgcga aacgctggga catcgtcgaa gccctcctcg
     2101 cccgcggcga ggacgcgaac gaccagaatc tccccttcct ctactggttc gccgccgaac
     2161 cgctcgtccc cgccgatccc gcgcgcgccc tcgccctcgc cggccaaacc ccgctcaccg
     2221 ccgt
//
LOCUS       DASPLT010000965         1066 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_298427, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000965 DASPLT010000000
VERSION     DASPLT010000965.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1066)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1066)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1066
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_298427"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(151..>1066)
                     /locus_tag="VEA63_10020"
     CDS             complement(151..>1066)
                     /locus_tag="VEA63_10020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007361109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="RNB domain-containing ribonuclease"
                     /protein_id="HYD84382.1"
                     /translation="HGSLDLDMPETKIFVDEQGYADRLEKIEHDESHQLIEEFMLAAN
                     EAVARLTRTHSLPSLYRVHDEPDPEKLAEYQGLLESFGIHTGDLTHREELVKLLNILR
                     DHPQGYTLRTQMLRSLKKAAYRGTHDGHFGLNKKDYTHFTSPIRRYSDLVVHRVFDFY
                     LVKHAGHPAAAGHQNYNIARIESLGEHLSLTEIASAEAERESVKIKLLEFFERELAKK
                     KKTHFAAVITDVRQNGFFVELIESMTFGFVSAATLNDDNYTLNNAGTALVGRKKKRLY
                     ALNGRLDVVVDRVDRFKRLIDFRPAPKV"
BASE COUNT          191 a          275 c          361 g          239 t
ORIGIN      
        1 gcacacgtcg ccgcgctcac aacacagagg cggatgggtt tcatgatgaa ccgggtttgg
       61 gcgcaacgta ggccgaagtg tccgcatttc aaagcggaat aacgccggcc gccggccggc
      121 gctcgagcag gcaggcgcgg aggcctgccg ctacaccttt ggcgcgggtc ggaagtcgat
      181 cagacgcttg aagcggtcga cgcggtcgac gaccacatcg aggcggccgt tgagcgcgta
      241 gaggcgtttc tttttgcggc caacgagagc cgtgccagcg ttgttcaacg tgtagttgtc
      301 gtcgttcaac gtggcggcgg agacgaagcc gaaggtcatc gattcgatca gttcgacgaa
      361 gaagccattt tggcggacgt cggtgatgac ggcggcgaaa tgcgttttct ttttcttcgc
      421 gagttcgcgc tcgaagaact cgagcagctt gatcttcacg ctctcgcgct cggcttcggc
      481 gctcgcgatt tcggtcaggc tcaggtgttc gccgagactt tcgatgcgcg cgatgttgta
      541 gttttggtgg ccggccgccg ccggatggcc ggcgtgtttc acgagataaa aatcgaagac
      601 gcggtgcacc acgagatccg agtagcggcg gatcggcgag gtgaagtggg tgtagtcttt
      661 tttgttgagg ccgaaatgac cgtcgtgcgt tccccgatac gcggcttttt tcaggctgcg
      721 cagcatttgt gtgcgcagcg tgtaaccctg tggatgatcg cgcaggatgt tgagaagctt
      781 aacgagttcc tcgcggtgcg tgagatcccc cgtgtgaatg ccgaagcttt cgagcaagcc
      841 ttgatactcg gcgagttttt cgggatcggg ctcgtcgtga acgcggtaaa gcgagggaag
      901 ggaatgggtg cgggtgagac gcgcaacggc ttcgttggct gccagcatga actcctcgat
      961 gagctggtga ctttcgtcgt gctcgatctt ttcgagccga tcggcgtagc cctgctcgtc
     1021 gacgaagatt ttcgtctcgg gcatgtcgag atcgagactg ccgtga
//
LOCUS       DASPLT010000966         1509 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_196733, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000966 DASPLT010000000
VERSION     DASPLT010000966.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1509)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1509)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1509
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_196733"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(212..>1509)
                     /locus_tag="VEA63_10025"
     CDS             complement(212..>1509)
                     /locus_tag="VEA63_10025"
                     /inference="COORDINATES: protein motif:HMM:NF023459.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YfbK domain-containing protein"
                     /protein_id="HYD84383.1"
                     /translation="YFPYRYAAPQGRPGEASAPFAATMEVAEAPWAPEHRLVRIGLKS
                     RDVATPDRAAANLVFLLDVSGSMSAANKLPLVKESMRLLLGRLRADDRVAIVTYAGAT
                     GLALPSTPVAKKREIEAAIAALTPNGGTNGAMGIQLAYDIAKANFVSEGINRVILCTD
                     GDFNVGVTSEGELVRLIEEKAKSGVFLTVLGFGMGNLKDATLQQIADRGNGSYGYVDT
                     RREAEKLLVEQVNGTLVTVAKDVKLQVEFNPAQVAKYRLIGYEKRLLAKEDFNNDQVD
                     AGEIGAGHTVTALYEIVPVAAKGSADGERGEGETASVDELRYSVVAVERVDVRTVVPG
                     AENELLTLKVRYKEPAGVVSRELEFSLTDTGAAFAEASEDFKFAAAVAGFGMLLRESQ
                     HKGAATFANVAAWAEAGAASDAGGYRAEFVELVRRAGAL"
BASE COUNT          266 a          622 c          344 g          277 t
ORIGIN      
        1 gtgagcagcc cccggatcgc gcaactcagc acgaatatga ggattgcatc caagtgccct
       61 cggctagacc ggacagcatt ggggtctccc gaccccgccg caaagctccg cgatccgcat
      121 ctcgcgctca ttcacgcgat cgcgaattca ccgtcgcaat ttagatcttc cctggagctt
      181 ggatcttggc gcttggagct ttctgcacct cttaaagcgc tcccgcccgc cgcaccagct
      241 ccacgaactc cgcgcgataa ccacccgcgt cgctcgcggc gcccgcttcc gcccacgccg
      301 ccacattcgc aaacgtcgcc gcgcctttgt gctgcgattc gcgcaacaac atcccgaatc
      361 ctgccaccgc cgcggcgaac ttaaaatcct cgctcgcttc agcgaacgcc gcgcccgtat
      421 cagtcagcga aaactccagc tcccggctca ccacacccgc cggctccttg tagcgcacct
      481 tcagcgtcag caattcgttc tccgcgcccg gcaccaccgt gcgcacgtcg acccgctcca
      541 ccgcgaccac gctataccgc aactcatcca cgctcgctgt ctcgccttca ccccgctcgc
      601 catccgcgct ccccttcgct gccaccggca cgatctcata aagcgccgtc actgtgtgac
      661 ccgccccgat ctcgcccgcg tcgacctgat cgttgttgaa atcctccttt gccagcagcc
      721 gcttctcgta gccgatcaac cgatacttcg cgacctgcgc cgggttgaac tccacctgca
      781 gtttcacgtc cttcgccacc gtcaccagcg tgccgttcac ttgctcgacc agcagcttct
      841 ccgcctcgcg ccgcgtatcc acataaccat aactgccgtt cccgcgatcc gcgatctgct
      901 gcaacgtcgc atccttcaaa ttccccatcc cgaaaccgag caccgtcaga aacacgcccg
      961 acttcgcctt ctcctcgatc aatcgcacca gctcgccctc gctcgtcacc ccgacattga
     1021 aatccccgtc cgtgcacagg atcacgcgat tgatcccctc gctcacgaaa ttcgccttcg
     1081 cgatatcata cgcgagctga atgcccatcg caccgttcgt ccctccgttc ggcgtcaatg
     1141 ccgcgatcgc cgcctcgatc tcgcgtttct tcgccaccgg ggtcgacggc agcgccagcc
     1201 ccgtcgctcc cgcatacgtc acgatcgcca cgcggtcatc cgcccgcaac cgtccgagca
     1261 acaaccgcat cgattccttc accagcggta gcttgttcgc cgcgctcatc gagcccgaca
     1321 catccagcag aaaaaccaga ttcgccgccg cccggtccgg cgttgcgacg tcgcgcgact
     1381 tcaggccgat ccgcaccagc cgatgctccg gcgcccacgg cgcctccgcc acttccatcg
     1441 tcgccgcaaa cggagccgac gcctcgcccg gccgcccctg cggtgccgcg taacgatacg
     1501 gaaaatagt
//
LOCUS       DASPLT010000967         1090 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_196763, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000967 DASPLT010000000
VERSION     DASPLT010000967.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1090)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1090)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1090
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_196763"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..296)
                     /locus_tag="VEA63_10030"
     CDS             complement(<1..296)
                     /locus_tag="VEA63_10030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD84384.1"
                     /translation="MSRYSREVSVAAVFGLLLLLLAMVAPAFFQGQPLLSLAAREAPT
                     LVVIGGMALVILCRQIDISVGSQFAVCGVAAGLLAGEGWPMVAVVIAAVLLGAI"
     gene            complement(483..>1090)
                     /locus_tag="VEA63_10035"
     CDS             complement(483..>1090)
                     /locus_tag="VEA63_10035"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HYD84385.1"
                     /translation="LARALFGLTPASGGKIVVGGSEAKIDSPRKAVELGIAYVPEDRR
                     RHGVILEMPIAENVTMAIHRRIFGGGWLRFGAERARAEEFVKKLAVKTPGVDALAGNL
                     SGGNQQKVALARWLATEPKVLILDEPTQGVDVGAKSEIHRIVRELANAGLAVILISSD
                     LPEAIGMSDRIGVMREGKLTCILPANAAAEDVMTAALGRNG"
BASE COUNT          225 a          439 c          246 g          180 t
ORIGIN      
        1 atgatcgcgc cgagcaacac cgccgcgatc accaccgcga ccatcggcca accctcgccc
       61 gccaacaacc ccgccgccac gccgcacacc gcgaactgcg aacccaccga aatgtcgatc
      121 tgccggcaca gaatcacgag cgccattccg ccgatcacca cgagcgtcgg cgcctcgcgc
      181 gccgcgagcg acaacaacgg ttgcccctga aaaaacgccg gggcgaccat ggcgagcaac
      241 aacaacagca gtccgaacac cgccgccacc gaaacttcgc gagaataacg gctcatgggg
      301 caaagctcca aatttgggcg aagctccaag cacgggcgaa gctccaagca ccgagctcca
      361 agctccagtg aaacttcaac ctccaaactt caaatacccg ctcactcgcc caccccacgc
      421 tcttgaagct tgaagtttga agtttcactg gagcttggat gttggagctt ggagctttca
      481 tttcacccgt tccttcccaa cgccgccgtc atcacatcct ccgccgctgc attcgccgga
      541 aggatacacg tcaattttcc ttcgcgcatc acgccgatcc gatcgctcat cccaatcgcc
      601 tccggcaaat cgctcgaaat caaaatcacc gccagccccg cgttcgccag ctcgcgcacg
      661 atccggtgaa tctcgctctt cgcccccacg tccacgccct gcgtcggctc gtcgagaatc
      721 agcaccttcg gctccgtcgc gagccaccgc gccagcgcca ccttctgctg attccccccg
      781 ctcagatttc ccgccagcgc gtccacgcct ggcgttttca ccgcgagttt tttcacgaac
      841 tcctccgcgc gcgcccgctc cgcgccgaac cgcagccacc ccccgccaaa gatccgccgg
      901 tgaatcgcca tcgtcacgtt ctccgcgatc ggcatctcga gaatcacgcc atgccgccgc
      961 cggtcctccg gcacatacgc aatacccaac tccaccgctt tccgcggcga atcgattttc
     1021 gcttccgaac ccccgacgac aatctttccg ccgctcgccg gcgtgagtcc gaacaacgcc
     1081 cgcgccagtt
//
LOCUS       DASPLT010000968         2449 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_113076, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000968 DASPLT010000000
VERSION     DASPLT010000968.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 2449)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2449)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2449
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_113076"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..288
                     /locus_tag="VEA63_10040"
     CDS             <1..288
                     /locus_tag="VEA63_10040"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HYD84386.1"
                     /translation="GPDGTEIPCPGVYLEVVPNERLVSTDAYTKPWEPSEKPFMTLIL
                     TFEDAGGGKTNYTARVLHWTEADREAHEKMGFHEGWTQCTEQLAALVEARG"
     gene            456..899
                     /locus_tag="VEA63_10045"
     CDS             456..899
                     /locus_tag="VEA63_10045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD84387.1"
                     /translation="MIRSLRRYLAVFVVACAAVGSAHAQVYTGAGTINLNAVSKSNFQ
                     IVTYLSIYNGSNYYTSVDIYLGNFSAHSSTNAGAADAFIYYNNVHKLPNGTWVAGNVT
                     IYTLDANWSWNTQVSYNVALPSSLSFGGIGYWGDAVSYQPYTIIN"
     gene            1235..>2449
                     /locus_tag="VEA63_10050"
     CDS             1235..>2449
                     /locus_tag="VEA63_10050"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HYD84388.1"
                     /translation="MKNITKHLTALAFVAGVGSLSAQTPPAASQNDQSDQDEVVRLSP
                     FNVSAGADIGRYTSLESTSAGRVRVSIMNSSQNVSVITSEMLSDVAAGRILDATKYIA
                     GISESTLPNAQDRTNVRGFQADGRTVDGFTYGGFMSMDPAIIDRIEVVKGPNSILAPQ
                     PTSPGGTVNNATKKPQFRDFGMINAQFGEFDANSGFLDINRQLNDTFAIRGVASFRDW
                     DNWWRDSSIRSTTLMPGLTWRISDTTELTVQYIYTEWRSSLYLGLPVDPSSGTKTKAH
                     ILAGVPKDLSVYADDIFRTDQQHDWKVLFTTKLWKGIDMRFSALYHKTSQYAPQLNTG
                     NSTGGVGGNRDPLTGFHEPGMQYLQTPPYTGSPIATQPTRVFVRSGTDPRADPRQLFL
                     QNDFAYFFENESL"
BASE COUNT          496 a          784 c          692 g          477 t
ORIGIN      
        1 gggccggacg gcacggagat cccgtgcccg ggcgtgtatc tcgaagtcgt gcccaacgaa
       61 cggctcgttt cgaccgacgc ttatacgaaa ccgtgggagc cttccgagaa gccgttcatg
      121 acgttgatcc tgacgtttga ggacgcgggc ggaggtaaaa ccaactacac cgcgcgcgtg
      181 ctgcactgga ccgaagccga tcgcgaggcc cacgagaaaa tgggttttca cgagggctgg
      241 acccagtgca ccgaacagct cgctgcgctc gtcgaagcgc ggggatgagc gaaattctgg
      301 cgcgaggggc gggagtttcc cgataagcgt tcccggcgtt gtcgcaaaaa ttttgcttgg
      361 agcggcggcg gtgggcgttt gtggtgtggg cgtcgtcgcg cgacgacact gcgacctaac
      421 tgtgcgtcgc agaaccccgc attaaacaac tccccatgat tcgctccctc cgtcgttacc
      481 ttgctgtgtt cgtcgtggct tgcgccgcag tcggttcggc tcacgcccaa gtctacacgg
      541 gcgctggcac catcaacctg aacgcggtct cgaaaagcaa ttttcagatc gtgacgtatc
      601 tcagcatcta caacggcagc aattactaca cgtcggtcga catctatctc ggcaacttct
      661 ccgcgcattc atcaacgaat gccggcgcgg ccgatgcgtt catctactac aacaacgtgc
      721 acaaacttcc gaacggcacg tgggtcgcag gcaacgtcac gatctacacg ctcgacgcga
      781 attggtcgtg gaacactcaa gtctcctaca acgttgcgtt gccgagctcg ctctcgtttg
      841 gcgggatcgg ttactggggc gacgccgtct cgtatcagcc ttacacgatc atcaactgag
      901 ttcgctgcag cagtagagca acgggagcgg tccatgcggg ccgctctttt tttgatgcca
      961 ggaagaagag gcagccagct ctccggtgag aacgaggccg cgtgtttttc gcatcatttg
     1021 ttttttcgcg tagccggttt tcacgcgcca cccgtcacaa cctcgcgccc cagatactcg
     1081 ccgtcttggt cacaccccaa aaaaccgcgt cggctggtcg ggcgcgcttc ttcgttcgac
     1141 gcacgttcac accccttttc caacccgttc gccaaagacg ccggatcgct ccggcgcact
     1201 gcggcgcgtg ggcgaaaccg acatcaaccc ctgcatgaaa aacatcacca agcacctcac
     1261 cgcgctcgcg ttcgtcgccg gcgtcggatc gctatcggcg cagaccccgc cggcagcttc
     1321 gcagaacgat caaagcgacc aggacgaagt cgtgcgattg tcgcccttca acgtcagcgc
     1381 cggcgcggac atcgggcggt atacttcgct cgaatcgacc tccgccggcc gcgtgcgcgt
     1441 cagcatcatg aattcgtcgc aaaacgtctc ggtcatcacg agcgagatgc tcagcgacgt
     1501 cgcggcgggc cgcattctcg atgcgacgaa atacatcgcc ggcatcagcg agtcgacgct
     1561 gcccaacgcg caagatcgca cgaacgtccg cggcttccag gccgatggcc gcaccgttga
     1621 tggtttcacc tacggcggtt tcatgagcat ggatccggcg atcatcgatc gtatcgaagt
     1681 cgtcaaaggt cccaactcga tcctcgcgcc gcaaccgact tcgcccggtg gcaccgtcaa
     1741 caacgccaca aagaagccgc agttccgcga cttcgggatg atcaacgcgc agttcggtga
     1801 gttcgacgcc aactccggct tcctcgacat caaccgccag ctcaacgaca cgttcgcaat
     1861 ccgcggtgtg gcgtcgtttc gcgactggga caactggtgg cgcgattctt cgattcgctc
     1921 gacgaccctg atgccgggcc tcacgtggcg aatttcggac acgacggaac tcacggttca
     1981 atatatctac acggagtggc ggtcgtcgct ttatctcggg ctgccggtgg atccatcttc
     2041 cggcacgaaa acaaaagcgc acatcctcgc cggcgtgccg aaggacctca gcgtttacgc
     2101 cgacgacatt ttccgcacgg atcagcagca cgattggaaa gtgctcttca cgacgaagct
     2161 ttggaaaggc atcgatatgc gtttctcggc gctgtatcac aagacgtcgc aatacgcgcc
     2221 gcagctcaac accggcaaca gcacgggcgg cgtcggcgga aatcgcgatc cgctcacggg
     2281 tttccacgag ccgggcatgc aatacttgca gacaccgcca tatacgggtt cgccgattgc
     2341 tacgcagccg acgcgcgtgt ttgtgcgttc gggcaccgac ccgcgcgcgg atccgcggca
     2401 actattcctc caaaacgact tcgcttactt cttcgaaaac gagtcgctg
//
LOCUS       DASPLT010000969         1391 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_12235, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000969 DASPLT010000000
VERSION     DASPLT010000969.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1391)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1391)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1391
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_12235"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            12..>1391
                     /locus_tag="VEA63_10055"
     CDS             12..>1391
                     /locus_tag="VEA63_10055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HYD84389.1"
                     /translation="MSVSVPQKIYSHEPVWDPARWLPTTRDEMDARGWDYVDVILVSG
                     DAYVDHPSFGTAVVGRMMEHAGFRVAILPQPNWRDDLRDFKKLGVPRMFFGVTAGCMD
                     SMVNRYTAAKKLRSEDAYTPGGEHGFRPDYASTVYSRILKQLYPDVPVLLGGIEASLR
                     RVTHYDYWSDTLKPGILEESGADLLVYGMGELPLREILRLLKRGVPFSSLKTIPQMAV
                     MLPAGAPLPKNGNWDDFTLHSHDECLRDRGLYSENFKNIETESNRVKARRLLQPVGER
                     MLVVNPPYPTMSEAQIDESFDLPYTRLPHPKYRKRGPIPAYEMIKHSINMHRGCFGGC
                     SFCTISAHQGKFVASRSKESILREVEQVKQMPDFRGTISDLGGPSANMYKMKGKQQWI
                     CDECVRPSCIWPDVCRNLDTDHTALLDIYQSVRETPGVKHAFVTSGIRYDLFLHDKGA
                     TPEVKASHEK"
BASE COUNT          273 a          347 c          489 g          282 t
ORIGIN      
        1 cggctcacac cgtgtctgtt tccgttccac aaaaaatcta ttcgcacgaa cccgtttggg
       61 atccggcgcg ctggctgccg accacgcgcg acgaaatgga cgcgcggggt tgggattacg
      121 tcgatgtgat tttggtgtcg ggcgacgcgt atgtggatca tccgtcgttc ggcacggcgg
      181 tggtggggcg gatgatggag cacgcgggat ttcgcgtggc gattttgccg cagccgaact
      241 ggcgcgacga tctgcgtgat ttcaaaaagc tcggcgtgcc gcggatgttt ttcggggtga
      301 cggcgggttg catggactcg atggtcaatc gctacacggc cgcgaaaaag ttgcggagcg
      361 aagatgctta tacgccgggc ggtgagcacg ggtttcggcc agattacgcc tcgacggttt
      421 actcgcggat tttgaagcag ctttatccgg acgtgccggt gttgcttggc gggatcgagg
      481 cgagtctgcg ccgcgtgacg cattacgatt attggtcgga cacgttgaag ccggggattc
      541 tcgaggagag cggcgcggac ctgctggttt acgggatggg cgagctgccg ctgcgggaaa
      601 tcctgcggct gttgaagcgc ggcgtgccgt tctcgtcgtt gaaaacgatt ccgcagatgg
      661 cggtgatgct gccggcgggc gcgccgttgc cgaagaatgg gaattgggat gacttcacgc
      721 tgcattcgca cgacgagtgc ctgcgcgacc gcgggttgta ttcggagaat ttcaagaaca
      781 tcgagaccga gtcgaatcgc gtgaaagcgc ggcggctgtt gcagccggtg ggcgagcgga
      841 tgctggtggt gaacccgccg tatccgacga tgagcgaggc gcagatcgac gagagcttcg
      901 atctgcctta cacgcggctg ccgcatccga aatatcgcaa gcgcggaccg atcccggcgt
      961 atgaaatgat caagcattcg atcaacatgc accgcgggtg tttcggcgga tgcagttttt
     1021 gcacgatctc ggcgcatcaa ggaaagttcg tcgcgagccg gagcaaggaa tcgattttgc
     1081 gcgaggtgga gcaggtgaag cagatgccgg atttccgcgg cacgatcagc gatctcggcg
     1141 ggccgtcggc gaacatgtat aagatgaagg gcaagcagca gtggatctgc gacgaatgcg
     1201 tgcgcccgag ctgcatctgg ccggatgtat gtcgaaattt ggatacagac cacacggcgc
     1261 tgctcgatat ttatcagtcg gtgcgcgaga cgccgggggt gaagcacgcg ttcgtgacga
     1321 gcgggattcg ctacgatctg tttttgcacg acaagggagc gacgccggaa gtgaaggcga
     1381 gtcacgaaaa g
//
LOCUS       DASPLT010000970         1113 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_176010, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000970 DASPLT010000000
VERSION     DASPLT010000970.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1113)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1113)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1113
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_176010"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            24..>1113
                     /locus_tag="VEA63_10060"
     CDS             24..>1113
                     /locus_tag="VEA63_10060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD84390.1"
                     /translation="MSASQRNTQTSARQHLAQITNGTWEIDEWADNMVWGRIYLDRPN
                     TFTDIPDGVNGYSVAYAPAQRYWFDFDDEFELTSYAAFSNTRLFNEDLSIVLGVRHDS
                     YDENLVSYRRALDFSDERVSESDEGTTYTVGAVYYFGWLGVFGNYSENILPPNAGSQP
                     YLNGDRPGPEQNEGYDYGLRISTDDGKYYASFSRYESTSNNRNVENPVDIRAVWQKYN
                     IARGVPQDEGFGAVAYSDTTAMDASGYEFELTANPLENLRIQASYALPETEIVEFYPA
                     ARAHVAENLATWNTQLAATTDPVHASDLRNEISRVEDKLAQSVPGVPQQKSVDYTASI
                     FANYTFTNEALDGYSIGAGGSYTGKPYAA"
BASE COUNT          247 a          395 c          290 g          181 t
ORIGIN      
        1 cgaacttcag ccaactcctc gccgtgtccg cgtcgcaacg caacacgcag acctccgcgc
       61 gccagcatct cgcgcagatc accaacggca cttgggaaat cgacgagtgg gccgacaaca
      121 tggtctgggg ccgcatctat ctcgaccgtc ccaacacctt caccgacatc cccgacggcg
      181 tgaacggcta ctccgtcgct tacgcgcccg cgcaacgcta ctggttcgac ttcgacgacg
      241 aattcgagct caccagctac gcggcgttct ccaacacccg cctctttaac gaggacctca
      301 gcatcgtcct cggcgtccgc cacgacagct acgacgaaaa cctcgtcagc tatcgccggg
      361 ctctggattt ctccgacgag cgcgtgagcg aaagcgatga aggcacgacc tacaccgtcg
      421 gcgccgtcta ctatttcggc tggctcggcg tgttcggcaa ctactcggaa aacatccttc
      481 cgcccaatgc cggctcgcag ccttacctaa acggcgatcg gccgggccct gagcaaaacg
      541 aaggttacga ctacggcctg cgcatttcca ccgatgacgg aaaatactac gcgagtttca
      601 gccgctacga atccacctcg aacaatcgca acgtcgagaa cccggtggac atccgggcgg
      661 tgtggcagaa atacaatatc gcgcgcggag tgccgcagga cgagggcttc ggtgccgtcg
      721 cgtattcgga cacgaccgcg atggacgcat cgggttacga gttcgaactc acggcaaacc
      781 cgctcgaaaa cctccgcatc caggcgagct acgcgctgcc cgaaaccgag attgtcgaat
      841 tctatccggc ggcacgcgct cacgtcgccg aaaacctcgc cacttggaat acccaactcg
      901 ccgcgacgac ggaccccgtc cacgcgagcg acctccgcaa cgaaatctcc cgcgttgaag
      961 acaagctcgc gcagtccgtt cctggcgttc cccagcaaaa atccgtcgat tacaccgcga
     1021 gcatctttgc aaactacacg ttcacgaacg aagcgctcga cggctactcg atcggcgccg
     1081 gcggcagcta cacgggcaag ccctacgccg cca
//
LOCUS       DASPLT010000971         1238 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_12260, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000971 DASPLT010000000
VERSION     DASPLT010000971.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1238)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1238)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1238
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_12260"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..351
                     /locus_tag="VEA63_10065"
     CDS             <1..351
                     /locus_tag="VEA63_10065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009511077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HYD84391.1"
                     /translation="VAQKLEIQPINVYDVVTFYPMFRQKPIGRRHIRVCRTLSCALRG
                     GYKTCEEFEKQFNTKVGEVSPDGEVTVEFVECLASCGTAPVVLIDDDLHEKVDCAKAK
                     ALSDQIKAEAAERR"
     gene            471..>1238
                     /locus_tag="VEA63_10070"
     CDS             471..>1238
                     /locus_tag="VEA63_10070"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373309.1"
                     /note="part of NADH-ubiquinone oxidoreductase complex I;
                     shuttles electrons from NADH, via FMN and iron-sulfur
                     (Fe-S) centers, to quinones in the respiratory chain; NuoF
                     is part of the soluble NADH dehydrogenase fragment, which
                     represents the electron input part of NADH dehydrogenase;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit F"
                     /protein_id="HYD84392.1"
                     /translation="MPAPEQRRIIFRHIDEPGYTNDIDCYLRNGGYEVLKKAVTLTPQ
                     SIIDEVKKSGLRGRGGAGFPCGVKWGLVDRKSGKPIYLIVNADESEPGTFKDRYIMHQ
                     DPHQLIEGTMISCFANNVKQAYIYIRGEMPHGARILERAIQEARDKNFVGQNILGTGY
                     SCDIFVHRGAGAYICGEETGLIESLEGKRANPRIKPPFFPAVLGLYQCPTIVNNVETL
                     CHVKYIAEHGGEAFTKIGTPNNTGTRIFCVSGHVQRPG"
BASE COUNT          272 a          368 c          362 g          236 t
ORIGIN      
        1 gtggcgcaga agctggagat tcagccgatt aacgtttacg acgtggtgac gttctacccg
       61 atgtttcggc aaaagccgat cgggcgtcgt cacatccggg tttgccgcac gctttcgtgc
      121 gcgctgcgcg gcggttacaa gacctgcgaa gagttcgaga agcagttcaa cacaaaggtc
      181 ggcgaggttt cgcccgacgg cgaagtcacg gtcgagttcg tcgagtgcct cgcgagctgc
      241 ggcaccgcgc cggtcgtgtt gatcgacgat gaccttcacg aaaaagtcga ctgcgcgaaa
      301 gcgaaagcgc tgagcgatca aatcaaagcc gaagcggcgg agcgccgctg aaccgatctt
      361 ttatcaacca cgaatggaca cgaatagaca cgaattaagc agcgcgttga gcagcgggtc
      421 tgctcattcg tgtgcattcg tgtgcattcg tggttaaaaa ctttccgtcc atgcctgctc
      481 ccgaacaacg ccgcatcatc tttcgtcaca tcgacgagcc cggctacacg aacgacatcg
      541 attgctacct tcgcaatggt ggctacgagg tgttgaagaa agccgtcacg ctcaccccgc
      601 agtcgatcat cgatgaagtg aagaagtcgg gtctgcgcgg ccgcggaggt gcaggtttcc
      661 cgtgtggcgt gaaatggggt ctcgtcgatc gcaagagcgg caagccgatt tatctcatcg
      721 tcaacgccga cgagtctgag cccggcacgt tcaaggatcg ctacatcatg caccaggatc
      781 cgcatcagtt gatcgagggc acgatgatct cgtgcttcgc gaacaacgtg aagcaggcct
      841 acatctacat ccgcggcgag atgccgcatg gcgcgcgcat cctcgaacgc gcgatccagg
      901 aagcgcgcga caagaatttt gtcggccaga acattctcgg cacgggttac tcctgcgaca
      961 tcttcgtgca ccgcggcgcc ggcgcttaca tttgcggcga agaaaccggc ctcatcgaat
     1021 cgctcgaagg caagcgcgct aacccgcgca tcaagccgcc gtttttcccc gcagtcctcg
     1081 gtctctatca gtgcccgacg atcgtgaaca acgtcgagac gctctgccac gtgaagtata
     1141 tcgccgagca cggtggcgag gcgttcacga aaatcggcac gccgaacaac accggcacgc
     1201 gcattttctg cgtcagcggt cacgtgcagc gcccgggc
//
LOCUS       DASPLT010000972         1030 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_298518, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000972 DASPLT010000000
VERSION     DASPLT010000972.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1030)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1030)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1030
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_298518"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(157..>1030)
                     /locus_tag="VEA63_10075"
     CDS             complement(157..>1030)
                     /locus_tag="VEA63_10075"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HYD84393.1"
                     /translation="GTRREVLRFATPTTAHNAQEIAFSPVADRADPDYALLYICIGDG
                     GAGNLGRPEMAGHPRTLFGAILRIDPAGNNSVNGRYGIPADNPFAESDDPAVHREVWA
                     YGFRNPHRISWDFAHGKRTIAVDIGEANIEEVNLIEKGGAYGWGAAGIEGHARIDVKT
                     DLKVVFPVTQAELTGVRLPFSEYDHLDGAAVTGGYVYRGPIKVLRDKYVFGDMVNGRL
                     FYLRLGAELTDRTIYELSVVRDGAVTSVKALANAQRAHLRIGYDERNGDLLILTKDDG
                     TLRRVSTAYERAEK"
BASE COUNT          186 a          374 c          264 g          206 t
ORIGIN      
        1 actgcccatc tccgcgctca aaaaagcgcg gctccgttga agcaggaact cttgagcttg
       61 cggtccgagt ctttcaccca tctccgcgct caaaaaagcg cggctccgtt gaagcgagcc
      121 gggcgctcct ccaccacatg cccgcctcgc tccgcctcac ttctccgccc gctcatacgc
      181 cgtgctcacg cggcgcagcg tgccgtcatc tttggtcagg atcaagaggt cgccattgcg
      241 ttcgtcgtat ccaattctca aatgcgcgcg ctgggcgttc gccagcgcct tcacgctcgt
      301 caccgctccg tcccggacca cgctgagttc gtagatcgtg cgatcggtca gttccgcgcc
      361 caagcgcaag tagaacaaac gcccgttcac catgtcgccg aacacatatt tgtcgcgcag
      421 caccttgatc gggccgcgat agacgtagcc gcccgtgacc gctgcgccgt caaggtgatc
      481 gtattcactg aacggcagcc ggacacccgt cagctccgct tgcgtcacgg gaaacaccac
      541 tttgaggtcc gttttcacgt cgatccgcgc atggccttcg atgcccgccg cgccccagcc
      601 ataggcgcct cctttttcga tcaggttaac ctcctcgata ttcgcctccc cgatatcaac
      661 cgcgatcgtc cgcttgccat gcgcgaaatc ccagctgatt cggtgcggat tccgaaatcc
      721 atacgcccac acttcgcgat gcaccgcggg atcatcgctc tccgcgaatg gattatccgc
      781 cgggattccg tagcgcccat tcacgctgtt gttgcccgcc ggatcaatac gcaggatcgc
      841 gccaaacagc gtgcgcggat gccccgccat ctccggacgg ccgagatttc ccgctccgcc
      901 gtctccgatg caaatataca acagcgcgta atccgggtcc gcgcgatccg ccactggcga
      961 aaacgcaatc tcctgcgcgt tatgcgcggt cgtcggtgtc gcgaaacgca acacctcgcg
     1021 acgcgttcct
//
LOCUS       DASPLT010000973         1642 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_154944, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000973 DASPLT010000000
VERSION     DASPLT010000973.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1642)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1642)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1642
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_154944"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1134)
                     /locus_tag="VEA63_10080"
     CDS             complement(<1..1134)
                     /locus_tag="VEA63_10080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD84394.1"
                     /translation="MLPLTACHPLLARFEDPAIKLASFDVFDTTITRAVGDPMCVFQL
                     VGTALQQRGVDISPDVFLHLRLAAERRTRSNRRGREVTLQEIYAEVAFALGREHEADA
                     LMAVELETEHSLVAPVPEMSELVDHARRRYGRVVYISDMYLPQDFLDERLRSFGLLEP
                     GDRLYVSSTCGGWKGDGRLFRTVLENEKLRPRELLHFGNSLPVDVRSAERLGIRAFHY
                     TPANPNASEEILNDYATHYGSTLSSLAGASRKVRLDAPQLCGEHRVVWDTGASLTGPL
                     LLMHATWMLERAVQCGIKRLLFLARDAYLPFKVMQRVLEIRRDLGIEAHYVYGSRATY
                     LPLGFTTFGEREWDSATTHGGAGYVTLSDLAQGLQLSRELLQPH"
     gene            complement(1157..>1642)
                     /locus_tag="VEA63_10085"
     CDS             complement(1157..>1642)
                     /locus_tag="VEA63_10085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD84395.1"
                     /translation="EQLDSPPSGEYTWPIQAVRLAVVLLFLGSGISKLVLGGWPWMFS
                     DNLYNIVSYCQLTRDTPLQIDIQSSLFYRTLAVGTVVIELAAPLALFHRASSTIIIPA
                     LLGMQIGIRLIMGDNFTQFMSLYLFWVPWMSLIVLVRRTSLSRNDRSQPVLGKPLSGH
                     L"
BASE COUNT          357 a          437 c          571 g          277 t
ORIGIN      
        1 gtgaggctgg aggagttcgc gtgagagctg caggccctgc gcgagatccg agagggtgac
       61 atagcctgcg ccgccatgag tcgttgcgct gtcccactcg cgttcgccaa acgtggtgaa
      121 accgagtgga aggtaggtcg cgcggctgcc gtaaacatag tgagcctcga tcccaagatc
      181 ccggcgtatc tcgaggacgc gctgcatgac cttgaacggg agatacgcat cgcgagcgag
      241 aaagaggagg cgcttgatgc cgcattgaac cgcgcgctcc aacatccagg tggcgtgcat
      301 gaggagcaat ggaccggtga gcgaagcgcc ggtgtcccag acgacgcggt gctcaccgca
      361 aagttggggt gcatcgagac ggactttacg cgaggccccg gcgagcgagc tcagcgtgga
      421 gccgtagtgc gtcgcgtagt cgttgaggat ttcctccgac gcgtttggat tcgctggcgt
      481 gtagtgaaag gcgcggatgc ccaggcgttc ggcactgcgg acatccactg gaagtgagtt
      541 gccgaagtgg agcagctcgc gcggccgtag cttctcgttt tccaacaccg tgcgaaaaag
      601 gcgtccgtcg cctttccaac cgccgcaggt cgacgacacg tagaggcggt cgccgggttc
      661 caacaagccg aatgaccgca gccgttcatc gaggaagtcc tgcgggagat acatgtccga
      721 aatatagacg acgcggccgt agcggcggcg ggcgtggtct acgagctcgg acatttcggg
      781 cacgggggcg acgagggagt gttccgtttc gagctcgacc gccatcagcg cgtccgcttc
      841 gtgttcgcgg ccgagggcaa acgcaacttc ggcatagatc tcctgtaagg tgacctcgcg
      901 accacgccgg ttggaacggg tccggcgttc tgccgcgagt cggaggtgaa gaaacacgtc
      961 ggggctaatg tcgacgccgc gctgctgcag tgcggtgccg acgagttgga acacgcacat
     1021 cggatcgccg acggcgcggg tgatcgtggt gtcaaacacg tcgaaggacg cgagtttgat
     1081 cgcgggatct tcgaatcgcg cgaggagcgg atgacatgca gtgagcggga gcataaccac
     1141 ggaagcatct gaaggatcag agatggccgg aaaggggttt gccgaggact ggctggctgc
     1201 ggtcgttgcg actgagcgaa gtgcggcgga cgagaacgat gagcgacatc cacggaaccc
     1261 agaaaaggta gagcgacatg aactgggtga agttgtcgcc catgatcaac cggatgccga
     1321 tctgcatgcc gagcagcgcg gggatgatga ttgtgcttga agcgcgatga aaaagagcga
     1381 gcggggcggc gagctcgatg accaccgttc cgactgcgag ggtgcgataa aacagcgagg
     1441 attggatatc gatttgcagc ggcgtatccc gggtaagctg gcaatacgaa acgatgttat
     1501 agaggttgtc ggaaaacatc cacggccacc cgcccagaac cagcttcgaa ataccggagc
     1561 caaggaatag gagaacgacc gcgagccgca cggcttggat cggccaagta tattcgccgc
     1621 ttggaggcga atccagctgc tc
//
LOCUS       DASPLT010000974         2570 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_12276, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000974 DASPLT010000000
VERSION     DASPLT010000974.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 2570)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2570)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2570
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_12276"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(7..567)
                     /locus_tag="VEA63_10090"
     CDS             complement(7..567)
                     /locus_tag="VEA63_10090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD84396.1"
                     /translation="MKQIAIIVVVVIAIATIAFSYRSYRAKSERELASRRANIEARET
                     EERRRAAAEQEAQRLAALQAEQEAAAAERAIAELRAQQEAVATRRATAEAEAARMQDE
                     LERLRQETAAMLAEVKEFSTTRPTDLTLVENAQRDAMEKLRELETEKVELADRQAAHA
                     EALKRQLEIEKQAQARVQRYRASLPR"
     gene            complement(564..1529)
                     /locus_tag="VEA63_10095"
     CDS             complement(564..1529)
                     /locus_tag="VEA63_10095"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HYD84397.1"
                     /translation="MSDAPHIYTLTGNLLAERTLEFAAWRAGHTQRASRETFQVGGKG
                     INVSKMLNRLHAANTALCFSGGAPGAECETWLRAQRFAFHAFATDLPTRSGTVVRAAD
                     QLETTFLGPDASPSASALKACADFLDAQPAGRVLALCGSFPGWTEPSFDPLRAALERW
                     LNRGILVADTYGAPLQWLARRPLALIKINAAEFRGVTSETAALPDALGRVSSAEPVHA
                     WIVTDGPAAVWFARKHDAPASLAPPRVREVSATGSGDVLFAAVLDALFRRGLSLRDAV
                     AAALPLAAANAAHPGIAEFPWEAIGIPPENEPARVAAVGSPSPSS"
     gene            complement(1526..>2570)
                     /locus_tag="VEA63_10100"
     CDS             complement(1526..>2570)
                     /locus_tag="VEA63_10100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007360334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HYD84398.1"
                     /translation="RWFIAWFLISSCFAAVAGVSWNAWIQEWVPARLRGKFFGRRNRF
                     LQLSTLVFMLLAGWVLARWEYAIPSFQAVVAIAVVMRVFSLRWQWKSPTRTLRRSIVP
                     TLPIGDQLKVVLNARSLLVFIAFGAVWSFAANCFGPFYHVFMFEQLDFTALDVGVFLT
                     LSQLGGALSLPAWGGLLDRYGNKSVMTFSLILWQTQYLLWCFITPENRNWLYAMWFWA
                     GMTSAGFVLGQFTILLRLIPVYAKNLAIGINLAATSLVAASAPIIGGWILSRALSGSA
                     DDLVVYHWCFLLQPILALVGCTLLLRVHEPQASPLTMVFGAMRNIRTLSGVLGLDFLI
                     NYVFYRPAAKTKR"
BASE COUNT          475 a          764 c          925 g          406 t
ORIGIN      
        1 gccgcgtcag cgcggcagcg aggcgcggta acgttgcacg cgcgcttgcg cctgcttttc
       61 gatctcgagc tggcgtttca gtgcttcggc gtgcgcggcc tgacgatcgg cgagctcgac
      121 tttctcggtt tcaagctcgc gcagtttttc catcgcgtcg cgctgggcgt tttcgacgag
      181 cgtgagatcg gtcgggcggg tggtggaaaa ttccttcact tcggcgagca tcgcggcggt
      241 ttcctggcgc agccgttcga gttcgtcctg catgcgcgcg gcttccgctt cggcggtggc
      301 gcggcgggtg gcgacggctt cctgctgtgc gcgaagctcg gcgatcgcac gctcggccgc
      361 ggcggcttcc tgttcagcct gcaacgcggc gaggcgttgc gcttcctgct ccgcggcggc
      421 gcggcgacgt tcctccgttt cgcgcgcctc gatgttcgcg cgacgcgacg cgagttcacg
      481 ctcggatttt gcccggtagc tccgatagct gaacgcgatg gtggcaatgg cgatcactac
      541 cacgacgatg atcgcgattt gtttcatgag gagggactcg gtgaaccgac agcggcgacg
      601 cgggcgggtt cattctcggg tggaatgccg atggcttccc aagggaattc agcgatgccg
      661 ggatgagcgg cgttggcggc ggcgagcggc agcgcggcgg ccacggcgtc gcggagtgag
      721 aggccgcgcc gaaacaacgc gtcgagcaca gcggcgaaca gcacgtcgcc ggaaccggtc
      781 gcggagactt ctcggacgcg cggtggcgcg agactggcgg gcgcgtcgtg cttgcgggcg
      841 aaccagacgg ccgcaggtcc gtcggtgacg atccaggcgt ggacgggttc ggcggacgaa
      901 acgcgaccga gtgcgtcagg cagcgcggcc gtttcgctgg tgacgccgcg aaattcagcg
      961 gcgttgattt tgattagcgc gagcggacgg cgtgcgagcc attgcagcgg cgcgccgtag
     1021 gtatcggcaa cgagaatgcc gcggttcaac caacgctcga gcgcagcgcg cagcggatcg
     1081 aaggagggct ccgtccatcc gggaaagctg ccgcaaagcg cgagcacgcg tcccgcgggt
     1141 tgagcatcga ggaaatcggc gcacgctttc agcgcggacg ctgacggcga tgcgtcgggt
     1201 ccgagaaatg tcgtctcgag ttgatcggcg gcgcgaacga cggtgccgct gcgcgtggga
     1261 agatccgtgg cgaacgcgtg aaaggcgaag cgttgcgcgc gcagccacgt ctcgcattcc
     1321 gcgccgggcg cgccgccgct gaagcagagc gctgtattcg ccgcgtggag acggttgagc
     1381 atcttggaaa cgttgatgcc cttgccgccg acttggaacg tttcgcgcga ggcccgctgc
     1441 gtgtgaccgg cgcgccatgc ggcgaactcc agcgtgcgtt cggcgaggag gttgccggtg
     1501 agcgtgtaga tgtgcggcgc gtcgctcatc gcttcgtctt cgcggccggc cggtagaaca
     1561 cgtagttgat gaggaagtcg aggccgagca cgccgctgag cgtgcggatg ttgcgcatgg
     1621 cgccgaacac catcgtgaga ggactcgcct gcggttcatg cacgcggagc agcagcgtgc
     1681 agccgacaag cgcgaggatc ggttgcagca gaaaacacca gtggtaaacg acaagatcgt
     1741 cggcggagcc gctcagggcg cgcgagagaa tccatccgcc aatgatcggc gcgctggccg
     1801 caacgagcga ggtggcggcg aggttgatgc cgatcgcgag gtttttcgcg tagaccggaa
     1861 tcaggcggag gagaatcgtg aactgcccga ggacaaaacc ggcgctggtc atgcccgccc
     1921 aaaaccacat cgcgtatagc cagtttcggt tctcgggcgt gatgaagcac caaagcagat
     1981 actgcgtttg ccagaggatg agcgaaaacg tcatcacgga tttgttaccg tagcggtcga
     2041 gcaagccgcc ccacgccgga agcgagagcg cgccgccgag ctgcgacagc gtgaggaaaa
     2101 cgccgacatc gagcgcggtg aagtcgagct gctcgaacat gaacacgtgg tagaacgggc
     2161 caaagcaatt cgccgcgaac gaccacacgg cgccgaacgc gataaacacg agcagcgagc
     2221 gcgcgttgag gacgactttg agctgatcgc cgatcggcag cgtcggcacg atcgagcgcc
     2281 gcagcgtgcg cgtggggctt ttccactgcc agcgcagcga gaacacgcgc atgaccaccg
     2341 cgatcgcgac gaccgcttga aaggacggga tcgcatattc ccagcgggcg agaacccagc
     2401 ccgccagcag catgaagacg agcgtggaga gctggaggaa gcgattgcgg cggccgaaga
     2461 atttgccgcg aaggcgcgcc ggcacccatt cctgaatcca cgcgttccag gagacaccgg
     2521 cgacggcggc gaagcagctc gaaatcaaaa accaggcgat gaaccagcgg
//
LOCUS       DASPLT010000975         3449 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_154952, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000975 DASPLT010000000
VERSION     DASPLT010000975.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 3449)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3449)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3449
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_154952"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..806
                     /locus_tag="VEA63_10105"
     CDS             <1..806
                     /locus_tag="VEA63_10105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD84399.1"
                     /translation="KLLRTPDAKQAAACEREAEEFLATIQKSFPRGPHRRFPGAIPAS
                     PHRRMDSGAVGSLVADYPLQLFAPGDASMAKTADYLCEHCLHGGGFFQNMIHSGINAY
                     LTLHLAQVRLRAGDARAAWALMDSVIGLASPTGQWPEAIHPLTLGGCMGDGQHIWAAA
                     EWLMFVRNLFVREESDALVIGAGVRPEWWRAGEVDFGPTLTPHGAVTVRLVGGESVVR
                     VNVEGQWRGGPPKRLLRVPGCVPLDIAAGNGSSDFELPLSEVATAEPRR"
     gene            995..2269
                     /locus_tag="VEA63_10110"
     CDS             995..2269
                     /locus_tag="VEA63_10110"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009508407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HYD84400.1"
                     /translation="MNICMMTNTYLPHVGGVARSVSTFAEEYRKRKHRVLVVAPEFEG
                     KSLPPRQAAIVERVPAIQKFNGSDFSVRLPIVAVPPRLDAFEADIIHAHHPFLLGDTA
                     VRIAATKNAPVIFTHHTRYEDYTHYVPFDSEALKEVAINLSTHFANLCDGVIAPSESI
                     AKLIKQRGVEVPIRVVPTGIDVGAFASGDGARFRRKFKLPADAFLVGHVGRLAPEKNL
                     EYLAEAVALFVKRTPEARFLVVGGGPSEEKLREVFAKHAVTDRLVMAGKQTGKALADA
                     YNAMDVFAFASFTETQGMVLSEAMAAGRPVVALNASGVREVMRDGKNGYMLAKNASAA
                     RFAAALDKLHGDAARRQAFSDEARRTAEMFSKERCAEQALEFYEDVRRMTRRERLMIE
                     RSPWGKLFERISVEWNLLAEKAQAVADAVGGR"
     gene            2433..>3449
                     /locus_tag="VEA63_10115"
     CDS             2433..>3449
                     /locus_tag="VEA63_10115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HYD84401.1"
                     /translation="MFAGIEAIVHRARRRLSRNEWAIRHLGLTPSEGTSEEPGLLLIQ
                     IDGLARRQLEAAMAQGRMPFLHRLMRRDDYTLHTFYPGLPSTTPAVQAELFYGVKSGV
                     PAFSFFDRGLKKLGRMWDPEWAKAREAAFSEKAEGLLKGGSSWSNIYTGGAGQEESHF
                     CAASIGLGDMWRSGKIRNIFLFIVLQLPAALRILWRLLVEMGVAISDAAQAIARGRKP
                     KLELMLLLSRVFVGVGLRELLTISGQIDVTRGLPIVHINFVGYDEHAHVRGPGSRFAL
                     YGLRGIDNAIRKIVRTAHRSRRRDYAVWVFSDHGQEGTRWFGDQHEGGIEAVLHECLE
                     LSKKR"
BASE COUNT          609 a          929 c         1280 g          631 t
ORIGIN      
        1 cgaagctgtt gcggacgccc gacgccaagc aggccgcggc atgcgaacgc gaagcggagg
       61 aattcctggc gacgattcag aaatcgtttc cgcgcgggcc gcaccggcgt tttccgggcg
      121 cgatcccggc gtcaccgcac cgccggatgg attcgggcgc ggtgggttcg ctcgtggcgg
      181 attatccgct gcagctattc gctccgggcg acgcatcgat ggcgaaaacg gcggattatc
      241 tttgcgagca ctgcctgcac ggcggcgggt ttttccagaa catgattcac tcggggatca
      301 acgcttacct cacgctgcac ttggcgcagg tgcggttgcg cgcgggcgac gcgcgggcgg
      361 cttgggcgtt gatggattcg gtgatcgggt tggcgtcgcc gaccgggcaa tggccggagg
      421 cgattcatcc gctgacgctc ggcggttgca tgggcgacgg gcagcacatc tgggcggcgg
      481 ccgagtggct gatgttcgtg cgcaatcttt tcgtgcggga agaaagcgat gcgctggtga
      541 tcggcgcggg tgtgcggccg gaatggtggc gcgcgggcga agtggatttc ggtccgacgc
      601 tgacgccaca cggcgccgtg accgtgcggc tcgtcggcgg tgaaagcgtg gtgcgcgtga
      661 acgtcgaggg acaatggcgc ggaggaccgc cgaagcgttt gttgcgcgtg ccgggttgtg
      721 tgccgctcga catcgcggcg gggaacggct cgtcggattt cgagttgccg ttgagcgaag
      781 tcgcgacggc ggagccgcgg cgttagcgga aaccttgttg tcggacggca gcgaggcgtt
      841 atggggaagc aggcaggcgc ggaggcctgc cgctacatgg ctgcaacgag gccttgacga
      901 acataccggc acgcgagcga gaaccgcaga gcggttgggc ggtctctgac ggcccggttc
      961 gcgctgcgcg accccgtgga aacgttttcc agcaatgaac atctgcatga tgaccaacac
     1021 ctacctgcca cacgttggcg gggtggctcg ctccgtgagc acgtttgccg aggaatatcg
     1081 gaaacgtaag caccgcgtgc tcgtggtcgc gccggagttc gagggcaagt cgctgccgcc
     1141 gcggcaggcg gcgatcgtcg agcgcgtgcc ggcgatccag aagttcaacg gcagcgattt
     1201 ctccgtgcgg ttgccgatcg ttgcggtgcc gccgcggctg gatgcgttcg aagccgacat
     1261 cattcatgcg catcatccgt ttttgctcgg cgacacggcg gtgcgaatcg cggcgacgaa
     1321 gaatgcgccg gtgattttca cgcaccacac gcgctacgag gattacacgc attatgtgcc
     1381 gttcgactcg gaggcgttga aggaagtggc gatcaatctc tcgacgcatt tcgcgaacct
     1441 gtgcgacggc gtgatcgcgc cgagtgagag cattgcgaaa cttatcaagc agcgcggcgt
     1501 cgaggtgccg attcgggtgg tgcccacggg gatcgacgtc ggcgctttcg cgagcggcga
     1561 tggcgcgcgg tttcggcgga agttcaaact gccggcggac gcgtttttgg tcggtcacgt
     1621 cgggcgtctc gcgccggaga agaacctcga gtatctcgcg gaggccgtgg cgctgttcgt
     1681 gaaacgcacg cccgaggcgc gttttctcgt ggtgggcggc gggccgtcgg aagagaaatt
     1741 gcgcgaagtg ttcgcgaagc atgcggtcac ggaccggctg gtgatggcgg gaaagcagac
     1801 gggcaaggcg ctggcggatg cttacaacgc gatggacgtg tttgcgtttg cgtcgttcac
     1861 cgagacgcaa ggcatggtgc tgtccgaagc gatggcggcg gggcggccgg tcgtggcgct
     1921 caacgcgtcg ggcgtgcgcg aagtgatgcg ggatggaaaa aatggataca tgctcgcgaa
     1981 gaacgcgtcg gccgcgcgct tcgcggcggc gctcgacaag ctgcacggcg acgccgcgcg
     2041 gcggcaggcg ttttcggacg aggcgcggcg aacggcggaa atgttttcga aggagcgctg
     2101 cgcggagcag gcgctggagt tttatgaaga cgtgcggcgg atgacgcgac gcgagcggtt
     2161 gatgatcgaa cgcagcccgt ggggaaaatt attcgagcgg atcagcgtcg agtggaacct
     2221 gctcgcagaa aaagcacagg cggtggcgga cgcagtggga ggacgatgac ggccggaacg
     2281 gccgaagctc caagctccaa gcaccaagat ccaagctcca atgaaattcc aaacaacaaa
     2341 caccgacagc cgagtggtga gcgtagaaag ttggttcatg gagcttggtg cttcactggt
     2401 gcttggagct tagtgcttgg agcttttcaa acatgtttgc cggaattgaa gcgatcgtgc
     2461 atcgggcgcg gcggcgattg agccgcaacg agtgggccat tcgtcatctg gggctcacgc
     2521 cgtcggaagg cacgagcgag gagccgggct tgctgttgat ccagatcgac ggacttgcgc
     2581 ggcgtcagct cgaggcggcg atggcgcagg ggcgcatgcc gtttcttcat cggctcatgc
     2641 ggcgcgacga ttacacgttg cacacgtttt atccggggct gccgtcgacg acgcccgcgg
     2701 tgcaggcgga gttgttctac ggagtgaagt caggcgtgcc ggcgttcagc tttttcgatc
     2761 gcgggctgaa aaagctcgga cggatgtggg atccggaatg ggcgaaggcg cgcgaggcgg
     2821 cattctccga aaaagcggag ggtttgctga agggcggcag ttcgtggtcg aacatttaca
     2881 cgggcggcgc ggggcaggag gaaagtcatt tttgcgcggc gagcatcggg ctcggcgaca
     2941 tgtggcgcag cggaaagatt cgaaacattt ttctgttcat cgtgctgcaa ctgccggcgg
     3001 cgctccgaat tctctggcgg ctgttggtgg aaatgggcgt cgcaatcagc gatgcggcgc
     3061 aggcgatcgc gcgcggacga aagccgaagc tcgaactgat gctgctgctc tcgcgcgtgt
     3121 ttgtcggcgt ggggctgcgc gagttgttga cgatcagcgg gcagatcgat gtcacgcgcg
     3181 ggctgccgat cgtgcacatc aacttcgtcg gctacgatga acatgcgcac gtgcgggggc
     3241 cgggctcgcg tttcgcgctc tacggcttgc gcggcatcga caacgcgatt cgcaagatcg
     3301 tgcgcacggc gcatcgctcg cggcggcgcg attacgcagt gtgggtgttt tccgatcacg
     3361 gtcaggaagg cacgcggtgg tttggcgacc agcacgaggg cgggatcgag gcggtgttgc
     3421 acgagtgcct ggagctttcg aaaaagcgc
//
LOCUS       DASPLT010000976         1076 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_135063, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000976 DASPLT010000000
VERSION     DASPLT010000976.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1076)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1076)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1076
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_135063"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1076)
                     /locus_tag="VEA63_10120"
     CDS             complement(<1..>1076)
                     /locus_tag="VEA63_10120"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HYD84402.1"
                     /translation="SGKARGVVVVFRDPAEMNLTPEELVKASRFESLGLLAGGIAHDF
                     NNLLTTILGGVSLAKDNRDYSALEDSEKACLTAKGLTKQLLMFAKGGSGTQIVVAAKD
                     ILDDAIKIAAAGADAQITVEVPEGVEPVRVDRAQILQVFQNLVVNALQAMPPAPHRAR
                     LQLRAANTKLADDQIAPLPAGDYVEFEVRDNGSGIAPENLQKIFDPFFTTKKHGTGLG
                     LATVLSIVRKHGGQIGVDSAVGTGTAFTVFLPRADAPVEVQARRAPSLRFGTGRILFM
                     DDDPKISALTATMLQSLEYKYDLAKNGEEAITLYKRYLNIGRPYDAVIMDITVIGGMG
                     GEECFKILRDLDPDVRAIVSSGYD"
BASE COUNT          180 a          292 c          382 g          222 t
ORIGIN      
        1 tgtcgtagcc gctcgagacg atcgcgcgca catcgggatc gagatcgcgg agaatcttaa
       61 aacattcctc gccgcccatg ccgccgatga cggtgatgtc catgatcacc gcgtcgtagg
      121 gccggccgat gttgaggtag cgtttgtaga gcgtgatcgc ctcctcgccg tttttggcga
      181 ggtcatattt gtattcgaga ctctgcagca tggtcgcggt gagcgctgaa attttcggat
      241 cgtcgtccat gaacaggatt cggccggtgc cgaaacggag cgacggcgcg cggcgggctt
      301 gcacctcgac gggcgcgtcg gcgcgcggaa gaaaaacggt gaacgcagtg ccggtgccga
      361 cggcggaatc gactccgatc tggccgccgt gtttgcgcac gatcgagagg accgtcgcga
      421 gaccgaggcc ggtgccgtgt tttttcgtcg tgaagaacgg atcgaaaatc ttctgaagat
      481 tttccggggc gatgccgctg ccgttgtcgc gcacttcgaa ttcgacgtag tcgccggcgg
      541 gcaatggcgc gatctggtcg tcggcgagct tcgtgttggc ggcgcggagc tggagtcgcg
      601 cgcgatgcgg cgcgggcggc atcgcttgga gcgcgttgac gacgaggttt tgaaacacct
      661 ggagaatttg cgcgcgatcg acgcggacgg gttcgacgcc ctcggggact tcgacggtga
      721 tctgcgcgtc ggcgccggcc gcggcaattt ttatggcgtc gtcgagaatg tctttggcgg
      781 cgacaacgat ctgcgttccg gagccgcctt tggcgaacat gagcaactgc ttcgtgagac
      841 ctttcgcggt gaggcaggct ttttcgctgt cttcgagggc cgagtagtcg cggttgtctt
      901 tcgcgagcga gacgccgccg aggatcgtcg taaggaggtt gttgaaatcg tgtgcgatgc
      961 cgccggcgag caagccgagc gattcgaagc gactggcttt gacgagctcc tcaggcgtga
     1021 ggttcatctc ggcggggtcg cggaaaacga cgaccacgcc gcgggctttg ccggag
//
LOCUS       DASPLT010000977         1890 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_257417, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000977 DASPLT010000000
VERSION     DASPLT010000977.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1890)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1890)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1890
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_257417"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..318
                     /locus_tag="VEA63_10125"
     CDS             <1..318
                     /locus_tag="VEA63_10125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD84403.1"
                     /translation="DAALHFPHTQILRHHLVRRQQRQPLNLRLRHEQAIERVLVERRQ
                     RQNSGRVFSRYCEFAPTIAKQAISQRRGINLEIGEALRALDRDFPYARGAEPNIRSCI
                     VDQ"
     gene            complement(390..647)
                     /locus_tag="VEA63_10130"
     CDS             complement(390..647)
                     /locus_tag="VEA63_10130"
                     /inference="COORDINATES: protein motif:HMM:NF014643.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HYD84404.1"
                     /translation="MKKPIAIVNMHEAKTHLSRLVEGAAKGEPFIIAKAGKPMVKVVP
                     LAEPTTKTTRLGFMKGQIKVPDDFDTMMQDEIIRLFEGEEE"
     gene            801..1709
                     /locus_tag="VEA63_10135"
     CDS             801..1709
                     /locus_tag="VEA63_10135"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HYD84405.1"
                     /translation="MRIATLNVQNLRLREDAGGPHFDGARDEIMPLSKLSAEARALDR
                     EDRRLTARLIADADADILALQEVFDQRTLDAFHDAHLAPLGAPYPHRICIPGNDGRRH
                     VALMSRLPLQDVQSHAAMSYADIGLDPPEGDAASARVFRRDCLAASCAGVQLYVVHFK
                     APSDPVSLSVVRREALSTRKLIERRFADPAAALWLILGDVNVNDVPTGDVIDVLTTDF
                     AHDLAAALPAAERWTYFHPAHISYARPDRMLASPLLAARCGAVEVRREGISRAAEACA
                     GPRLAGVGVIRPRASDHALLAVDVHG"
BASE COUNT          331 a          699 c          515 g          345 t
ORIGIN      
        1 gacgccgcgt tacactttcc gcacacccaa atacttcgcc accatcttgt ccgacgtcag
       61 caacgtcagc cgctcaatct gcgcctgcgc cacgagcagg cgatcgaacg ggtccttgtg
      121 gagcggcggc agcgacagaa cagcggccgc gtgttctccc gttactgcga gttcgctcca
      181 accattgcta agcaagccat ttcgcagcgc cgcgggatca atctggaaat cggcgaagcc
      241 cttcgcgcgc ttgatcgcga cttcccatat gctcgcggcg ctgaaccgaa catccgcagc
      301 tgcatcgtcg atcaatgacc gcgcagtctt cgaaagcttt gccggatcct ccacgcccca
      361 gagtatgaga tgcgtgtcaa cgagcagcct cactcctcct cgccctcgaa caggcggatg
      421 atctcatcct gcatcatcgt gtcgaaatcg tcggggactt tgatctgacc cttcatgaag
      481 cccagccgcg ttgtcttcgt cgtcggctcc gccagcggca ccaccttcac catcggcttg
      541 ccggccttag cgatgatgaa cggctcgcct ttggccgcgc cttccaccag acgcgacagg
      601 tgcgtcttgg cttcatgcat gttcacgatg gcgatcggct tcttcatgga cttagtccac
      661 taaacttagt ctagcccggc cctatggtca accgccccgt atagagccta aggaacacct
      721 gcgcgccggc cggctccctc gcttggactg cggcggcttg aagccatcgc catcacgccg
      781 gcgcgcgcct atcaggcccc atgcgcatag cgacgctcaa cgtccaaaac ctgcgtctgc
      841 gcgaagacgc cggcggcccc cacttcgacg gcgcccgcga cgaaatcatg ccgctctcaa
      901 aactcagcgc cgaagcgcgc gcgctggatc gggaggatcg ccgcctcacc gcgcgcctca
      961 tcgcagatgc agacgccgac atcctcgcgc tgcaggaagt gttcgaccag cgcacgctcg
     1021 acgccttcca cgacgcccat ctcgcgccgc tcggtgcgcc ttatccgcat cgcatctgca
     1081 tccccggcaa tgacgggcgc cgccacgtcg cgctgatgag ccgcttgccg ctgcaagacg
     1141 tgcaaagcca cgccgccatg agctacgccg acatcggtct cgatccgccc gaaggcgacg
     1201 ccgcttcagc gcgcgtcttt cgccgcgact gcctcgctgc gtcctgcgcc ggcgtccagc
     1261 tctacgtcgt ccacttcaaa gcgccgtccg atcccgtgag cctctccgtc gtacgccgtg
     1321 aagcattgtc cacgcgcaag ctaatagaac gccgcttcgc cgatcccgcc gcggccctct
     1381 ggctgatcct cggcgacgtc aatgtgaacg acgtcccaac cggcgacgtg atcgacgtac
     1441 tgacgacgga tttcgcgcac gatttagccg ccgcgcttcc agccgccgaa cgctggacct
     1501 actttcaccc cgcccacatc agctacgccc gccccgaccg catgctcgcc tcacctctgc
     1561 tcgctgcgcg ctgcggcgcc gtcgaagtgc gccgcgaagg catctcgcgc gccgcggaag
     1621 cttgcgccgg tccgcgtctt gccggcgtcg gcgtcattcg cccccgcgcc agtgaccacg
     1681 cgctgctcgc cgtcgatgtg cacggatgaa acggtgaata cacgcccggc gtgaccatcg
     1741 tcacttgccg cgcattcctt actgcatcga cacgcattgc tcacaaggcc cgctgctagc
     1801 ttcgctccac ttcgcaagca gcgaggggag atcaagatga gtttcgacat gtcgaaggtc
     1861 gtcgccgaat tgggcgacga agtgatcgcc
//
LOCUS       DASPLT010000978         1130 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_51268, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000978 DASPLT010000000
VERSION     DASPLT010000978.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1130)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1130)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1130
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_51268"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..517
                     /locus_tag="VEA63_10140"
     CDS             <1..517
                     /locus_tag="VEA63_10140"
                     /inference="COORDINATES: protein motif:HMM:NF024240.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HYD84406.1"
                     /translation="AAEIDGRVQALEYIRFLRDKVPGYEKAQLATFGTQIGVRETRRV
                     YGDYRLTRNDVLSAKQFDDQIALCGAPIEDHHGGTDTKWAYLPEGRCVGVPFRTLVPR
                     DGQNVLVAGRCFSASHDAHAAVRSMAQTMAMGQAAGTAAAIAAKGDGTIRSVNVSQLQ
                     IQLRRDGALLE"
     gene            517..>1130
                     /locus_tag="VEA63_10145"
     CDS             517..>1130
                     /locus_tag="VEA63_10145"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HYD84407.1"
                     /translation="MKTPPVWIGLDIGGTRLKGGAFDDHGRVIARDIEPTRPQQSHAA
                     LERQLNALVSRLLRTAKTNTPAGIGAAITGPVDPERGCVYLPGKIAGLDRHATVPFLS
                     KRWRAPAIADNDGRLACYAEWRAGAGRGVDNLVVFTLGTGIGSGVVLDGRLLTDRRHM
                     RGTQCGHLVIEVGGPRCLTGARGTGESLASVTALVNSVRDALARG"
BASE COUNT          193 a          398 c          354 g          185 t
ORIGIN      
        1 ggctgccgag atcgacggcc gcgtgcaggc gctcgaatac attcgtttcc tccgcgacaa
       61 agttcccggc tacgagaaag cgcagctcgc cacgtttgga acgcaaatcg gcgtgcgcga
      121 gacgcgtcgc gtttacggcg actatcgtct cacccgcaac gacgtgcttt ccgcgaagca
      181 attcgacgac cagatcgcac tctgcggcgc gccgatcgag gatcaccacg gcgggaccga
      241 cacaaagtgg gcttatctgc ccgaaggccg ctgcgtcggc gtgccgtttc gcacgctcgt
      301 tccgcgcgat ggacaaaacg tgctcgtcgc cggccgctgt ttctccgcgt cgcacgatgc
      361 gcacgccgcg gttcgttcga tggcgcaaac catggcgatg ggccaggcgg ccggcacggc
      421 cgcggcgatc gccgcgaaag gcgacgggac catccgttcc gtcaacgttt cccaactcca
      481 aatccaactc cgccgcgacg gcgcgcttct cgaatagtga aaaccccacc cgtttggatc
      541 ggcctcgata tcggcggcac gcgcttgaaa ggcggtgcgt ttgacgacca cggtcgcgtg
      601 atcgcgcgcg acatcgaacc gacgcgcccg caacaaagtc acgctgcgct cgagcgacag
      661 ctcaacgcgc tcgtctcgcg tctgctgcgc acagcgaaaa cgaacacccc cgcgggcatc
      721 ggtgctgcca tcaccggccc ggtcgatccg gaacgcggtt gtgtttacct gcccggcaaa
      781 atcgccggcc tcgatcgtca cgcgaccgtg cctttcttga gcaaacgctg gcgcgcgcct
      841 gcgatcgccg acaacgacgg ccgcctcgca tgctacgcgg aatggcgcgc cggagcgggt
      901 cgcggcgtcg acaatcttgt ggtgtttaca ctcggcactg gaatcggctc cggcgtcgtc
      961 ctcgacggcc gtttgctcac ggatcgccgg catatgcgcg gcacgcagtg cggtcatctc
     1021 gtcatcgagg tcggcggccc gcgctgcctc accggcgcac gcggcaccgg cgaaagtctc
     1081 gcgtccgtga ccgcgctggt gaacagcgtg cgcgacgccc tcgcacgcgg
//
LOCUS       DASPLT010000979         1222 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_12368, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000979 DASPLT010000000
VERSION     DASPLT010000979.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1222)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1222)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1222
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_12368"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..413
                     /locus_tag="VEA63_10150"
     CDS             <1..413
                     /locus_tag="VEA63_10150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009513643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="mucin desulfatase"
                     /protein_id="HYD84408.1"
                     /translation="EGICVIDLDTVMPGLALYDFGDMVRSATNSAAEDECDLARVHSR
                     LSIFEALVDGYLSSARTFLNEAEIAHLALSGRLITFEIGIRFLTDFLDGDVYFKTKRP
                     GHNLDRARNQFALVRSMEAQQAEMETIVRRLAKR"
     gene            410..>1222
                     /locus_tag="VEA63_10155"
     CDS             410..>1222
                     /locus_tag="VEA63_10155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009513642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HYD84409.1"
                     /translation="MKVLITGGAGFIGSHLAEHFQGKGTVRVLDNLRTGHRQNLAGLE
                     VEFIEGSITDRAVVRRAMEGVDYVFHLAALVSVPESVERPRDCVELNVLGLLNVLDEA
                     SAAGVRKLVFSSSAAIYGNNPVVPKREDMLPEPCSPYAVTKLDGEYYCQHYAAAGRIE
                     TASLRYFNVFGPRQDPAGAYAAAVPIFIHRALGHQPLTIYGDGGQTRDFIFVKDIVAA
                     NVFAATTPGVTGVFNAGYAGQITVLELAQRIIALAGSKSEIRFAPERAGDVRH"
BASE COUNT          222 a          324 c          426 g          250 t
ORIGIN      
        1 acgaaggcat ttgcgtgatc gatctcgaca cggtgatgcc gggcctggcg ctttatgatt
       61 tcggcgatat ggtgcgctcg gcgacaaatt cggcggcgga agacgaatgc gatctcgcgc
      121 gcgtgcattc gcggctgtcg atcttcgagg cgctggtgga tggatatctg tcgtcggcgc
      181 gaaccttcct gaatgaggcg gagatcgcgc atctcgcgtt gtcgggacga ttgataacgt
      241 tcgagatcgg cattcgattt ctcaccgatt tccttgatgg cgacgtgtat ttcaaaacga
      301 agcggccggg acataatctc gatcgggctc gcaaccaatt cgctctcgtg cgcagcatgg
      361 aggcgcagca agccgagatg gaaacgatcg tgaggagact ggcgaagcga tgaaagttct
      421 gatcaccgga ggcgcaggtt ttatcggcag ccatctcgcc gaacattttc aagggaaggg
      481 cacggtgcgg gtgctcgaca atctgcgcac ggggcatcgg cagaatcttg ccggtctcga
      541 ggtggaattc atcgaaggct ccatcacgga tcgcgcggtg gtccgtcgcg cgatggaagg
      601 cgtggattat gtgtttcatc tggcggcgct ggtgagcgtg ccggaatcgg tggagcgacc
      661 gcgggattgc gtggagttga acgtccttgg tttgttgaac gtgctcgacg aggcgtcggc
      721 agcgggggtg cgcaaactgg tgttcagcag ctcggcggcg atctatggca acaatccggt
      781 ggtgccgaag cgggaagaca tgctgccgga gccgtgcagc ccgtatgcgg tgacgaagct
      841 cgatggcgag tattactgcc agcactacgc ggcggccggg cggatcgaga cggcgtcgct
      901 gcggtatttc aacgtctttg gtccccgcca ggatcccgcg ggcgcttatg cggcggcggt
      961 gccgattttc attcatcgcg ccctcgggca ccagccgctc acgatttacg gcgacggcgg
     1021 acagacgcgg gatttcatct tcgtgaagga catcgtggcg gcgaacgtct tcgcggcgac
     1081 gacgccggga gtgacggggg tgttcaacgc cggctatgca ggccagatca ctgtcctcga
     1141 gctcgcgcag cgcatcattg ctctggcggg atcgaaatcg gagatccggt tcgcgcccga
     1201 gcgtgcgggc gatgtgcgcc at
//
LOCUS       DASPLT010000980         1151 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_257474, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000980 DASPLT010000000
VERSION     DASPLT010000980.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1151)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1151)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1151
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_257474"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            178..>1151
                     /locus_tag="VEA63_10160"
     CDS             178..>1151
                     /locus_tag="VEA63_10160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD84410.1"
                     /translation="MNTPVFVSRSRRFAASCLALLTLGLPPALALPAFPGAEGFGANA
                     TGGRGGSVYIVTNLNDSGAGSFRDAVSQPNRTVVFAVGGIIPISSRIVVRENITIAGQ
                     TAPGEGITLYGNGVSFSNANNTICRYIRFRQGLGGDAGTDAVGIASGDSMIFEHVSAS
                     WGRDETFSVSGTPSNITLQDCIVGQGLLAHSAGGLMQTDGGVSVFRTLYTDNWMRNPK
                     VKGVHEFTNNVVYNWGGGGGYILGDSAGQSYANISHNYFIAGPNSPAQAFTRGNLNFH
                     VFADNNLHDANRNGVLDGAVVPPDGYTTVDFVPNRYPYPAVATLLTPD"
BASE COUNT          196 a          428 c          299 g          228 t
ORIGIN      
        1 atcgttcgcg atctccgagc tcttgaccgc aaaaactctg acggagcgaa attcgaacgc
       61 tcgaactccg cgcgctccag gcgtcctttg cgtgcattac cccgccggat cgctggagct
      121 ttcgcgtttc atcgtccgac gttcctcccc tggttcttca ctcgaacgtt tccgtttatg
      181 aacacccccg ttttcgtttc acgctcacgt cgtttcgcgg cctcgtgtct cgcgcttctc
      241 accctcggac tccccccggc gcttgctctt cccgccttcc ccggcgcgga gggtttcggc
      301 gccaacgcca ccggcggccg cggcggctcc gtttacatcg tcacgaatct caacgacagc
      361 ggcgccggtt cgttccgcga cgccgtcagc cagccgaacc gcaccgtcgt tttcgccgtc
      421 ggcggcatca tcccgatcag ctcccgcatc gtcgtgcgcg aaaacatcac tatcgccggt
      481 cagaccgcgc ccggcgaagg catcaccctt tacggcaatg gcgtctcgtt ctccaacgcg
      541 aacaacacca tctgccgcta catccgcttc cgtcagggcc tcggcggcga tgccggcacg
      601 gacgccgtcg gcatcgcgtc gggcgactcg atgatcttcg aacacgtatc cgcatcttgg
      661 ggacgcgatg agacgttctc cgtcagcggc accccgagca atatcacatt gcaagactgc
      721 atcgtcggcc agggcctgct cgcgcactcc gccggcgggc tcatgcagac cgacggaggt
      781 gtcagcgttt tccgcacgct ttacaccgac aactggatgc ggaacccgaa ggtgaaaggc
      841 gtccacgagt ttaccaataa cgtcgtctac aactggggcg gcgggggcgg ctacatcctt
      901 ggcgactccg ccggccagtc ctacgcgaac atcagccata actacttcat cgccggtccg
      961 aactcgcccg cgcaggcctt cactcgcggc aatctcaact tccacgtttt cgccgacaac
     1021 aatctccacg acgccaaccg caacggtgtg ctcgacggcg ccgtcgtccc gccggacggc
     1081 tacacgaccg tcgacttcgt tccgaaccgt tatccgtatc ccgccgtcgc cacacttctg
     1141 accccggatc a
//
LOCUS       DASPLT010000981         1309 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_51284, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000981 DASPLT010000000
VERSION     DASPLT010000981.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1309)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1309)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1309
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_51284"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..369)
                     /locus_tag="VEA63_10165"
     CDS             complement(<1..369)
                     /locus_tag="VEA63_10165"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024913.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HYD84411.1"
                     /translation="MCDPAASRIRLRAASGADASLLRRWDEAPHILASDPNDDWQWET
                     ELGRRVDWREQLVAELDGRPLGYVEIIDAAREEDHYWGEVPPGTRAIDIWIGDAADLG
                     KGYGTRMMTLALARCFADPVT"
     gene            complement(362..760)
                     /locus_tag="VEA63_10170"
     CDS             complement(362..760)
                     /locus_tag="VEA63_10170"
                     /inference="COORDINATES: protein motif:HMM:NF037170.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HYD84412.1"
                     /translation="MQVLINIDVPDLEQGIAFYQRALPLTLARRMFDGTVAEMRAENV
                     RIYLLEKPQGSAPFTHAEARDYGRHWTPVHLDFAVGDIETALTQALKAGAKQETEVDR
                     AEWGAIVTLSDPFGNGFCLVRFEGGVYEHV"
     gene            complement(765..1256)
                     /locus_tag="VEA63_10175"
     CDS             complement(765..1256)
                     /locus_tag="VEA63_10175"
                     /inference="COORDINATES: protein motif:HMM:TIGR00040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HYD84413.1"
                     /translation="MKPSWDGRIAMLRIGLISDTHGLLRPQAAAFLQGCDRILHAGDI
                     CSAETLPLLSALAPVTAVRGNNDYGAWADALRETERLDIGGLAVYVIHDLAELDIDPA
                     AAGVRIVVYGHSHQPHVDERAGVLYVNPGSAGPRRFRLPVSVGELIIDEGRPSVRLVE
                     LEV"
BASE COUNT          242 a          443 c          398 g          226 t
ORIGIN      
        1 cgtcacagga tcggcgaagc agcgcgccag cgcgagcgtc atcatgcgcg tgccgtaacc
       61 cttgccgagg tccgcggcgt cgccgatcca aatatcgatc gcccgcgtcc cgggcggcac
      121 ctcgccccag tagtggtctt cctcgcgcgc cgcatcgatg atttccacat agccgagcgg
      181 ccgcccgtcc agttcggcaa cgagttgctc ccgccaatcc acgcgccgcc cgagctcggt
      241 ctcccactgc cagtcgtcgt tcggatcgga agcaaggatg tggggcgctt cgtcccaccg
      301 ccggagcagc gaggcgtcgg caccggaggc ggcgcgaagc cggatgcgcg acgctgcagg
      361 atcacacatg ttcgtaaacg cctccctcga agcgcaccag acaaaacccg ttgccgaaag
      421 ggtcactcag cgtgacgatg gcgccccact cggcacgatc cacttccgtt tcctgtttcg
      481 ctccggcttt caacgcttgt gtcagcgccg tctcgatgtc tcccaccgcg aaatcgagat
      541 gcacgggcgt ccagtgcctg ccgtagtcgc gtgcttcggc atgcgtgaac ggtgcgctgc
      601 cttgcggttt ttccagcaga tagatgcgga cgttctccgc gcgcatttcc gcgaccgtgc
      661 catcgaacat tcgccgtgca agcgtgagcg gcaacgcccg ctgataaaag gcaatgccct
      721 gctcgagatc cggcacatcg atgttgatca gcacctgcat cgcgtcagac ttccagttcc
      781 accagacgca cgctcggccg gccttcgtcg atgatcagtt cgccgacgga aaccggcagc
      841 ctgaagcgcc gcggcccggc gctgcccgga ttgacataga gcacgcccgc gcgctcgtcc
      901 acatgaggct gatgcgaatg cccgtagacc acgatacgca cgcccgcggc tgccggatcg
      961 atatcgagtt cggcaagatc atggatgaca tagaccgcaa gaccgccgat atcgagtcgc
     1021 tccgtttcgc ggagcgcgtc ggcccaggcg ccgtagtcgt tgttgccgcg caccgccgtg
     1081 acaggcgcta acgcggaaag cagaggaagg gtctccgcgc tgcagatatc gcccgcatgg
     1141 agaatccggt cgcagccttg cagaaacgca gccgcctgcg gccgaagcaa accgtgggta
     1201 tccgaaatca gtccgatgcg caacatggcg atgcggccgt cccatgaagg tttcacgtcg
     1261 acagggtcag cggcgattgg ttcggcgcgt caatgcatgg ctcggaatt
//
LOCUS       DASPLT010000982         1235 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_35351, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000982 DASPLT010000000
VERSION     DASPLT010000982.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1235)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1235)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1235
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_35351"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..368)
                     /locus_tag="VEA63_10180"
     CDS             complement(<1..368)
                     /locus_tag="VEA63_10180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HYD84414.1"
                     /translation="MRVIDVHTHPIFRQDDRGVAGAAQLIRRARAHGIEHIIALGDVL
                     AFGRLPTEAQVRRINDETAELMRAYPEFVTGFCHLNPTLGERAVRREVARCAARGFRG
                     LKLEITNNARDACMRPLMEEA"
     gene            469..>1235
                     /locus_tag="VEA63_10185"
     CDS             469..>1235
                     /locus_tag="VEA63_10185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HYD84415.1"
                     /translation="MATSLKTDRDIEWLLSTPTPAVTAAVARLPGDFVVLGVGGKMGT
                     TTALMLRRALDAAGRRESNVLGVSRFSRAEARHELEELGIRTLACDLADAAQVAALPA
                     TANVLYLAGQKFGTDSAPELTWIQNTVVPAFVAQRFRDSRIVVFSTGCVYPFVPTDGP
                     GANESEPVAFLGEYASTCVGRERVFTHFSKQFGTPQLIYRLNYAVELRYGVLVDIAQK
                     VQRGETVDVTMGWLNCIWQGDAYAHAIQCLEHTASPPL"
BASE COUNT          228 a          475 c          319 g          213 t
ORIGIN      
        1 gcctcttcca tcaacggccg catgcaagcg tcacgcgcat tgttcgtgat ctccagtttc
       61 aacccacgaa accctcgcgc cgcacaacgc gccacctccc gccgcaccgc gcgttcgccg
      121 agcgttggat tcaaatgaca aaagcccgtc acgaactccg gatacgcccg catcaactcc
      181 gccgtttcgt cgttgatccg ccgcacctgc gcctccgtcg gcaaacgtcc aaacgccagc
      241 acgtcgccca gcgcgatgat gtgctcgatc ccatgcgccc gcgcccgtcg gatcaactgc
      301 gccgcccccg cgacgccgcg gtcgtcctgc cggaaaatcg ggtgcgtgtg aacatcgatg
      361 actcgcatga aatttcagtg gattcacgct tggcaaatgg cgacgcgcga gccggattac
      421 ctctgaccaa ccggctcacc aattaaaggc aaatccggct gaaatttcat ggcgacctcc
      481 ctcaaaaccg accgcgacat cgaatggctg ctctccactc ccacgcccgc ggttaccgcc
      541 gccgtcgcac gtttgcccgg tgatttcgtg gtcctcggcg tcggcggaaa aatgggcacc
      601 accaccgcgc tcatgctccg ccgcgccctc gacgccgccg gccgccgcga gagcaatgtc
      661 ctcggcgtct cccgtttcag ccgcgccgaa gcacgccacg aactcgaaga actcggcatc
      721 cgcacgctcg cctgcgatct cgccgacgcc gcgcaagtcg ccgcgctccc cgcgaccgcc
      781 aacgtcctct acctggccgg ccaaaaattc ggcaccgaca gcgcgcccga gctcacgtgg
      841 attcaaaaca ccgtcgtgcc cgccttcgtc gcccagcgtt ttcgcgactc gcgcatcgtc
      901 gttttctcca ccggctgcgt gtatccgttc gtccccaccg acggccctgg cgccaacgaa
      961 agcgagcccg tcgcgttcct cggcgaatac gccagcacgt gcgtcggtcg cgagcgcgtg
     1021 ttcactcatt tctccaaaca attcggcacg ccccagttga tctaccgtct caactacgcc
     1081 gtcgagctgc gctacggcgt gctcgtcgac atcgcgcaaa aggtccaacg cggcgaaacc
     1141 gtcgacgtta cgatgggctg gctcaactgc atctggcagg gcgacgccta tgcacatgcc
     1201 atccaatgtc tcgaacacac cgcgagcccg ccgct
//
LOCUS       DASPLT010000983         2126 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_12393, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000983 DASPLT010000000
VERSION     DASPLT010000983.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 2126)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2126)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2126
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_12393"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            195..1760
                     /gene="metG"
                     /locus_tag="VEA63_10190"
     CDS             195..1760
                     /gene="metG"
                     /locus_tag="VEA63_10190"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HYD84416.1"
                     /translation="MKSFYITTAIDYVNGSPHLGHAYEKVLTDVVARFRRQMGDKVYF
                     LTGTDEHGQKVQASARKEGIEPQAFADKVSAEFRSMLGRLNISNDDFIRTTEDRHKKV
                     VRQILQQLFDKGEIYKTEYRGFYSTRQEQFLQEKDRLPDGTWPEIFGEVTEIVEPNYF
                     FKLRQYQDWLIEFLTKNEDFVFPRFRQKQVLEFLKEPLNDLCISRPKDRLEWGISLPF
                     DEDYVTYVWFDALLNYYSAVADKGLWPADFNVIGKDILVPPHAVYWPIMLHAAGLPPP
                     KRLLVHGWWLQRGSKMSKSTGNALNPLDLVDEFGVDAFRYFLIREMNVGQDSDFTREQ
                     FLVRYNSELANNLGNLVNRTLNMTTRFAGGVLPAAEAAAAEAAGVEDKAEEELRTLWD
                     KTRDECVALNEEFQFHTALERTMQFVTATNAYIERRAPWKLGKSTEAKDQALLRTSLA
                     TMAEALRLAVALMQHVTPTTTEKINAVLGYTPGADWRAEMEWGAKLSGAKVAGALVLF
                     PRPEKPGVDTKKD"
     gene            1847..>2126
                     /locus_tag="VEA63_10195"
     CDS             1847..>2126
                     /locus_tag="VEA63_10195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009509437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase"
                     /protein_id="HYD84417.1"
                     /translation="MTILPLDPAANATLEALRAFLATCREAARKDERAKLVSISIGVD
                     ALDPLAVLESIFEPGEQHFYAERPSLDTAIAGAEAVVSLDASGPGRFAA"
BASE COUNT          420 a          504 c          787 g          415 t
ORIGIN      
        1 ggttttgggg atggcagagg gggatgggcc ttatgggacg aatgggactg ataggactga
       61 taggacgggt gggacttatg ggatggctgg ggatgcgttg gcttcggggt tttttagaat
      121 gcgatggggc ggagggccgt ggagcaaaac tttcgacatt tgggcggggc ggcgtttcgt
      181 taaacgactt ccgcatgaag tcattctaca tcacgaccgc catcgactat gtgaacggtt
      241 cgccgcactt gggccacgcc tacgaaaaag tgctgacgga cgtcgttgcc cggtttcggc
      301 ggcagatggg ggacaaggtg tatttcctga ccggcaccga cgaacacggg caaaaagtgc
      361 aggcgagtgc gcggaaggaa gggatcgaac cgcaggcgtt tgcggacaag gtgagcgcgg
      421 agtttcgctc gatgctcggg cgattgaaca tctcgaacga cgacttcatt cgcacgacgg
      481 aggaccggca caagaaagtc gtgcggcaga ttttgcagca gctcttcgac aagggcgaaa
      541 tctacaagac ggagtatcgc ggcttttact cgacgcggca ggagcagttt ttgcaggaga
      601 aggatcggct gccggacggg acgtggccgg agattttcgg cgaggtgacg gagatcgtgg
      661 agccgaatta tttcttcaag ctgcggcagt atcaggactg gctgatcgag tttctgacga
      721 agaacgagga cttcgttttc ccgcggtttc ggcagaagca ggtgctggag tttttgaagg
      781 agccgttgaa cgacctctgt atttcgcgac cgaaggatcg gctcgagtgg ggcatctcgc
      841 tgccgttcga cgaagattac gtgacgtatg tgtggttcga cgcgctgctg aattactatt
      901 cggcggtggc ggacaaggga ttgtggccgg cggatttcaa cgtgatcggc aaagacatcc
      961 tcgtgccgcc gcacgcggtt tactggccga tcatgctgca tgcggccggg ctgccgccgc
     1021 cgaagcgatt gctggtgcat gggtggtggt tgcaacgcgg atcgaagatg tcgaagagca
     1081 cgggcaacgc gctcaacccg ctcgatctcg tggacgagtt cggcgtggat gcgtttcgtt
     1141 attttctgat tcgcgagatg aacgtggggc aggacagcga tttcacgcgc gagcagtttc
     1201 tcgtgcgcta caacagcgaa ctcgcgaaca atctcggcaa tctcgtgaac cgcacgttga
     1261 acatgacgac gcggttcgcg ggcggtgtgc tgccggcggc cgaagcggct gcggccgagg
     1321 cggcgggggt cgaggacaaa gcggaggagg agctgcggac gctgtgggac aagacgcgag
     1381 acgagtgcgt ggcgttgaac gaagagtttc agtttcacac ggcgctggag cggacgatgc
     1441 agttcgtgac ggcgacgaat gcttacatcg agcggcgcgc gccgtggaag ctcggcaagt
     1501 cgacggaagc gaaggatcag gcgctgttgc ggacgtcgct cgcgacgatg gcggaagcgc
     1561 tgcggctcgc ggtggcgttg atgcagcatg tgacgccgac gacgacggag aagatcaacg
     1621 cggtgctcgg ctacacgccg ggcgcggact ggcgcgcgga gatggagtgg ggcgcgaagt
     1681 tgagcggcgc gaaagtagcg ggcgcgttgg tgttgtttcc gcggccggag aagccgggcg
     1741 tggatacgaa gaaggactga tcgttctctt tctcgtaatc gttctcgttc tctcgtgggc
     1801 tgagagaatc gagaacgaga acgagaacga cgaacgagaa cgatatatga cgatccttcc
     1861 gctagatcct gctgcgaatg ccacgctgga ggcgctgcgg gcgtttttgg cgacgtgtcg
     1921 tgaagcggcg cggaaggatg agcgcgcgaa gctggtgagc atttcgatcg gggtcgacgc
     1981 gctcgatccg ctggcggtgc tggagtcgat tttcgagccg ggagagcaac atttttatgc
     2041 ggagcggccg tcgctggaca cggccatcgc gggggcggaa gcggtggtgt cgttggatgc
     2101 gtcggggcca gggcggttcg cggcgg
//
LOCUS       DASPLT010000984         1266 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_35382, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000984 DASPLT010000000
VERSION     DASPLT010000984.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1266)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1266)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1266
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_35382"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..700)
                     /locus_tag="VEA63_10200"
     CDS             complement(<1..700)
                     /locus_tag="VEA63_10200"
                     /inference="COORDINATES: protein motif:HMM:NF014662.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HYD84418.1"
                     /translation="MNELLAIVRRITASIESAGVLATLVSVAGSSYRRPGARLLLTPH
                     GERLGSISGGCLEEDVLARARHVAATGAPDLVTYDTTSENDLVWGVGLGCHGVVQVFI
                     EKLAPQPRWAATLAANLRANAPTPLAVVWRSPHAAHLGTHLADEAPSPSSSNSAIFLN
                     TIAPPIPLAIFGAGDDAQPLTRLAKELGWHVTVADPRRDFATPARFPAADKIVVAPSH
                     QLVAESALPPGALAV"
     gene            1043..>1266
                     /locus_tag="VEA63_10205"
     CDS             1043..>1266
                     /locus_tag="VEA63_10205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD84419.1"
                     /translation="MPSLVIAATLIPVLGFAQGADADPADDPVRLEKFEVTGSYIPFS
                     ADAPAVPVRIVGSQEIAATGITNDLLEVIR"
BASE COUNT          226 a          335 c          480 g          225 t
ORIGIN      
        1 cgacggcgag tgcgccgggc ggaagcgcgc tttcggcgac gagctggtga gatggcgcaa
       61 cgacgatctt gtcggcggca gggaagcggg cgggagtggc gaaatcgcgg cgggggtcgg
      121 cgacggtgac gtgccagccg agttccttcg cgagacgcgt gagcggttgc gcatcgtcgc
      181 cggcgccgaa gatcgcgagc gggatgggcg gagcgatcgt gttgagaaaa attgcggagt
      241 tggaagagga aggggaggga gcttcgtcgg cgaggtgggt gccgaggtgg gcggcgtggg
      301 gcgagcgcca cacgaccgcg agcggcgtgg gggcgttggc gcggaggttg gcggcgagcg
      361 tcgcagccca gcgtggctgg ggcgcgagtt tttcgatgaa cacttggacg acgccgtggc
      421 agccgaggcc gacgccccag acgaggtcgt tttccgaagt cgtgtcgtag gtgacgaggt
      481 caggcgcgcc ggtggcggcg acgtgtcggg cgcgggcgag gacgtcttct tcgaggcatc
      541 cgccgctgat cgagccgagg cgttccccat ggggcgttag gaggagtcgc gcaccggggc
      601 gccggtagga agagccggca acggagacca atgtcgccaa cacgccggcc gattcgatgg
      661 acgcggtgat gcggcggacg atggcgagga gttcgttcac gatgcgaggg cggccatcac
      721 tcttctacgg tgcgggcgcg gggcaagcgc ggttgcgtag gggcggaggt ctggcgatcg
      781 gcagcgagtg tttttgaaag gcccgccgca ggaaatcggc gctctgccgc gcgcagccgg
      841 cgttggtcgt cataattccg cgttctaacc aaattattgc ttttattatt tttcgtcaca
      901 cgttgccgca agttcaggcg gctgcgaagt tcgaatgaac ttacacaaca caactcggcg
      961 gtttcctctc gggagaaacg tcgaacacac aacgcaactg attacgacta tgaacaaatt
     1021 ccgctcgcgg aagttctgtg cagtgccgtc gcttgttatc gcggcgacgt taattccggt
     1081 gcttgggttc gcccaaggag ccgacgccga tccggcggat gatccggtga gactggaaaa
     1141 attcgaagtt accgggtcct acattccgtt ttcggccgac gcgccggcgg tgccggtgcg
     1201 tatcgtagga tcgcaggaga tcgcggcgac cggcatcacc aacgaccttc tcgaggttat
     1261 tcggaa
//
LOCUS       DASPLT010000985         1281 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_176167, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000985 DASPLT010000000
VERSION     DASPLT010000985.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1281)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1281)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1281
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_176167"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..667
                     /locus_tag="VEA63_10210"
     CDS             <1..667
                     /locus_tag="VEA63_10210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD84420.1"
                     /translation="KARATLAPDAVLDAVKTIHYTGTLISADPADRTKELTRQIEIFL
                     EKPARQRIVVTSPQVIEVSALDEYEAWRRTTDATDSTKWQQQQLSVDQIKQLRADVWQ
                     NLYFFRGIERVGGRVEDQGLATIDGIECQKIAFFHSPTLVYYRYFNRATGELVLTGDE
                     NSNVREQGEILAGGIRFPKAILITQKLADGQNDTRRITFEKVAVNEAFPASLFAVPLP
                     TLK"
     gene            750..>1281
                     /locus_tag="VEA63_10215"
     CDS             750..>1281
                     /locus_tag="VEA63_10215"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007359957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HYD84421.1"
                     /translation="MRILPHASKNFDAELASFCRTTVVPREISDSVADILADVQTRGD
                     EAVVYYAAKFDGAKLRARELRVKPADLAAAAKRLPAADRRAIVAARDSIVAFNRRGLP
                     SDWTAKNPHGAIVGEKFDPLRRVGLYVPGGQVPLVSTVLMTVALAKIAGCPEVAVFTP
                     SNASGKIADGLLAALHL"
BASE COUNT          232 a          415 c          394 g          240 t
ORIGIN      
        1 aaaggcccgt gcgaccctcg cgcccgatgc cgttctcgat gcggttaaaa cgattcatta
       61 caccggaacg ttgatcagcg ccgatccggc cgatcgcacg aaggagctca cgcgccagat
      121 cgagattttt ctcgagaagc ccgcgcgcca gcggatcgtc gtcacttcac cgcaagtgat
      181 cgaggttagt gcgctcgacg aatacgaggc ctggcgccgc acgaccgacg ccaccgattc
      241 gacgaagtgg cagcagcagc aactctccgt cgaccagatc aaacaactgc gcgccgacgt
      301 gtggcagaac ctttatttct ttcgcggcat cgagcgcgtc ggcggccgcg tcgaggatca
      361 aggcctggcg acgatcgatg gcatcgagtg tcagaagatc gcgtttttcc acagcccgac
      421 gctcgtctat tatcgttatt tcaatcgggc cacgggcgaa ctcgtcctca ccggtgacga
      481 aaacagcaac gtgcgcgaac aaggcgaaat cctcgcgggc ggcattcgtt tcccgaaagc
      541 catcctcatc acgcaaaagc tggccgacgg ccagaatgac acgcgccgga tcacgttcga
      601 gaaggtcgcg gtcaacgagg cgtttcccgc gagccttttc gccgttccgc ttccgacgct
      661 gaaatgattt tcgttttcgc cggcggcctc ggtcgcgcgg cctcgagcct ttaaccaagc
      721 cccgctgcgg ggcgttccgc ccgtctgtca tgcgcatcct ccctcacgcc tccaaaaatt
      781 tcgacgccga gctcgcgagc ttctgtcgca ccaccgtcgt gccgcgggag atttccgatt
      841 cggtggccga catccttgcc gatgtgcaga cgcgtggcga cgaagcggtc gtgtattacg
      901 cggcgaaatt cgacggtgca aaattgcgcg cgcgcgagtt gcgggtgaag ccagcggatc
      961 tcgccgccgc ggcgaagcgc ctgccggctg ccgaccgccg cgcgatcgtt gcggcgcgcg
     1021 acagcatcgt tgcgttcaac cgccgcggat tgccgtcgga ttggacggcg aagaatccgc
     1081 acggcgcgat cgtcggcgag aagttcgatc cattgcgccg cgtcggtctc tacgtgcccg
     1141 gcggccaggt gccgctcgtg tcgacggtgt tgatgacggt cgcgctcgcg aagatcgcgg
     1201 gatgcccgga ggtcgcggtg ttcacacctt cgaatgcttc gggcaaaatc gcggacggtt
     1261 tgctcgctgc gctgcacctg c
//
LOCUS       DASPLT010000986         1140 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_215845, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000986 DASPLT010000000
VERSION     DASPLT010000986.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1140)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1140)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1140
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_215845"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(151..735)
                     /gene="coaE"
                     /locus_tag="VEA63_10220"
     CDS             complement(151..735)
                     /gene="coaE"
                     /locus_tag="VEA63_10220"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375890.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HYD84422.1"
                     /translation="MILGITGGIGCGKSTAAALFEKRGFRRLDSDAIIRDEVLTSADV
                     QAALRARYGEDVVKPDGTVDRARVAAHVFSDETELQWLEALTHPALFALWRSRFAVAP
                     NADWAVEVPLLFEKRLEIWFDFTICVAIAPAQQLARLEQRGLSRALAEQRISKQLPLA
                     HKIELADFVLWNDGTPEFLEAQVDRLVESLPVAR"
     gene            848..>1140
                     /locus_tag="VEA63_10225"
     CDS             848..>1140
                     /locus_tag="VEA63_10225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD84423.1"
                     /translation="MFPRVDTKNPSAVAALVRERFGALFPGAETTWFDRLFADVETMF
                     AGRHPDYAAIDLRYHDLEHTLQATVCLTLLLEGCQASGALPKMTARQFELAVAS"
BASE COUNT          217 a          332 c          365 g          226 t
ORIGIN      
        1 cggcgacgcg tggtcttctt cttctcgccg gcattactgt cgccggctgg agagttttcg
       61 tcgtcgtttt tcgaaggcat atcaaattct ggcaataaag agtgaccccg ggattggggg
      121 tgaaaaaagg ggaaccgaaa accgattttc tcagcgggcc accggcagcg actcgacgag
      181 ccggtcgact tgcgcttcga gaaactcagg ggtgccgtcg ttccaaagga cgaaatcggc
      241 caattctatt ttgtgcgcca atggcagttg cttggaaatt cgttgctcgg cgagcgcgcg
      301 agaaaggccg cgttgctcca gtcgggcgag ttgctgagcc ggagcaatgg cgacgcagat
      361 cgtgaaatca aaccaaattt cgagacgctt ttcgaagagc agtggcactt cgaccgccca
      421 atcggcattt ggtgcgacgg cgaagcgcga acgccagagc gcaaacaacg ccggatgcgt
      481 gagtgcctcg agccactgga gttccgtttc atcggaaaaa acgtgcgcgg cgactcgggc
      541 gcggtcaacg gtgccgtcgg gcttcacgac atcctcgcca tagcgtgcac gcagcgcggc
      601 ttgaacgtcc gccgaggtga gcacctcgtc gcgaatgatc gcgtcggaat ccagccggcg
      661 aaaaccgcgc ttttcgaaca acgcggcggc cgtcgacttg ccgcaaccga tccctcccgt
      721 gatgccaaga atcatgtgtg gatgccggag agctttcggc ttgcgcagcc atttcgcaaa
      781 ttcttaatgg agccgcgctg ccgcactcca gttcccgcgg tccggcgatc ctcccttcaa
      841 ctgccgtatg tttccgcggg tcgacacgaa gaacccctcc gccgtggcgg cgctggtgcg
      901 cgagaggttt ggagcgcttt ttccgggggc cgagacgacg tggttcgacc gattgtttgc
      961 ggacgtggaa acgatgtttg ccggccggca cccggattac gcggcgatcg atctgcggta
     1021 tcacgatctc gagcacacgt tgcaggccac ggtgtgcctg acgctgctgc tcgaaggctg
     1081 ccaggcgtcg ggcgctttgc cgaagatgac ggcgcggcag ttcgagctcg cggtggcgtc
//
LOCUS       DASPLT010000987         1276 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_215851, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000987 DASPLT010000000
VERSION     DASPLT010000987.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1276)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1276)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1276
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_215851"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..298)
                     /locus_tag="VEA63_10230"
     CDS             complement(<1..298)
                     /locus_tag="VEA63_10230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HYD84424.1"
                     /translation="MPTVSGVLLIGLSLGIGLAAYNASNNILFITLSLLLACLILSGV
                     LSWLNFRRVSWRLQVAPPLRAGQEAVVTLEVRNGKRVLPTYGLWFDVAARSLEGD"
     gene            complement(576..>1276)
                     /locus_tag="VEA63_10235"
     CDS             complement(576..>1276)
                     /locus_tag="VEA63_10235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007360621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HYD84425.1"
                     /translation="IYDETVKEFVFKKGPVFANIVLADEINRATPKTQSALLEVMDRA
                     RVTVDGEAHATGAPFMVFATQNPVDYEGTFPLPESQMDRFLMRLQMGYPQTEDELEIL
                     RTARTSYDAIALEPVLSQSEVLRLQALAAQVFVEDSVLEYLLKIVAATRTEAEFRAGV
                     SVRGGLALRVAAQARALVNGRDFVLPEDVTELVLPVFAHRLGLARQSSDALEERRTVA
                     AALRRIVGAIPAVV"
BASE COUNT          269 a          486 c          303 g          218 t
ORIGIN      
        1 catcgccttc gagcgaccgc gccgccacgt cgaaccacaa cccatacgtc ggcagaacgc
       61 gcttcccatt ccgcacctcc aacgtcacca ccgcttcctg cccggcccgc agcggtggcg
      121 ccacctgcaa tcgccacgac acccgccgga aattcagcca cgacaacacg ccgctcaaaa
      181 tcaaacacgc gagcagcaac gagagcgtga tgaacaaaat attgttcgaa gcattatacg
      241 ccgccagccc gatcccgagc gacaacccga tcagcaacac gccggatacc gtcggcaaga
      301 tgcgctgcct cccctgcgga taaattaaag accagagcag cgcgctccat ttaaaattcg
      361 cgcgccgtcc gctccggctc gtgccggtcc acattcctga atcagaaccg ttcatgtgcg
      421 cgctgaagct ccaagcacca agctccaagc tccaaagaaa cttcaaactc cagccgccaa
      481 cccagctcca tctgacgtga ttgaatcatt gaatcttggt gtttccctgg agcttggagc
      541 ttggagcttg gaatttggag ctttcgcggc ggcgctcaca ccaccgccgg aatcgccccc
      601 acaatccgcc gcagcgccgc cgcgaccgtt ctccgctcct ccaatgcatc gctcgattgc
      661 cgcgccagcc cgagccgatg agcgaacacc ggcagcacca attcggttac atcctccggc
      721 agcacgaaat cacggccgtt caccaacgcc cgcgcctgcg ccgccacgcg caacgccagc
      781 ccgccgcgca cgctcacgcc cgcgcgaaac tccgcctccg tccgtgtcgc cgccacgatc
      841 ttcagtaaat attccaacac gctgtcctcg acgaacacct gcgccgcgag cgcttgcagc
      901 cgcagcacct cgctctgcga caacaccggc tccagcgcga tcgcatcgta gctcgtccgc
      961 gccgtccgca aaatctccag ctcgtcctca gtttgtggat accccatctg caggcgcatc
     1021 aaaaaccggt ccatctggct ctccggcagc ggaaacgtcc cttcgtaatc caccgggttt
     1081 tgcgttgcga acaccatgaa cggcgcgccg gtcgcatgcg cctcgccgtc gaccgtcaca
     1141 cgtgcgcgat ccatcacctc gagcagtgcc gactgcgtct ttggcgtcgc gcgattgatc
     1201 tcgtccgcga gaacgatgtt cgcaaacacc ggccctttct tgaacacgaa ctccttcacc
     1261 gtctcgtcgt aaatcg
//
LOCUS       DASPLT010000988         1052 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_113395, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000988 DASPLT010000000
VERSION     DASPLT010000988.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1052)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1052)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1052
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_113395"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..621)
                     /locus_tag="VEA63_10240"
     CDS             complement(<1..621)
                     /locus_tag="VEA63_10240"
                     /inference="COORDINATES: protein motif:HMM:NF015971.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD84426.1"
                     /translation="MKPYRTTVLSLMRGLSVLALLASGARAEIAEWMIGPFDRPENAA
                     PVIAPNPASVFDCPMRKKPVHWEAKHTFNPAAVVKDGKIFVLYRAEDDSGRTYAGYTS
                     RLGLAWSEDGIHFTRHPTPVLYPDNDDEKTREWEGGCEDPRVVELEDGSYAVFYTQYH
                     RLPEEKDWSVTIGVATSADLFHWTKQGPIVAKLPGGGERRPRKSASL"
     gene            complement(661..1050)
                     /locus_tag="VEA63_10245"
     CDS             complement(661..1050)
                     /locus_tag="VEA63_10245"
                     /inference="COORDINATES: protein motif:HMM:NF012579.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HYD84427.1"
                     /translation="MLAELLAADDRISDSVAIEEAVWIREDAYPTGFGHGFAMPHCKS
                     PSVRANSIALLRLNQAVEWGSIDDAPVDVVILLAVREADHRKEHMRVFSRLSRLVLRE
                     EFRHALMSTTDPAAICQVLQQQLAPAA"
BASE COUNT          198 a          344 c          308 g          202 t
ORIGIN      
        1 cagcgacgcc gatttgcggg gacgccgctc ccctcccccg ggcagcttcg cgacgatcgg
       61 tccctgcttc gtccagtgaa aaagatcggc cgaggtcgcc acgccgatgg ttacactcca
      121 gtccttctcc tcgggcagcc ggtgatactg ggtgtagaaa accgcatagc tcccgtcctc
      181 gagttcgacc actcgcggat cttcgcagcc accttcccac tcgcgggttt tctcgtcgtc
      241 gttatccgga tacaacaccg gagtcgggtg gcgcgtgaaa tggatgccat cctcgctcca
      301 ggccagacct agccgcgacg tataacccgc ataggtgcgg ccgctgtcgt cttccgcccg
      361 atagagcacg aaaattttcc catctttcac cacggcggcg ggattgaacg tgtgcttcgc
      421 ctcccaatgc accggcttct tccgcatggg gcagtcgaag actgacgccg gattcggcgc
      481 gatcacgggc gccgcatttt cagggcggtc gaagggccca atcatccatt ccgcgatttc
      541 ggcccgcgcg ccactcgcca gaagagcgag cacgctcaag ccgcgcatga gagacaaaac
      601 agtcgtgcga tatggtttca tgttgaagaa aggtgggccg agcggcggcg tgcttttcat
      661 tcaagccgcc ggtgcgagct gctgctggag cacttggcag atggccgcag gatcggtcgt
      721 gctcatcagc gcgtgtcgga attcctcgcg caacacgaga cgcgacaacc gcgaaaacac
      781 tcgcatgtgc tccttgcggt ggtccgcttc acgcacggcg aggagaatca ccacatccac
      841 cggagcatca tcgatcgaac cccactcgac cgcttggttg agccggagca gagcgatgct
      901 gttcgcgcgc acgcttggac ttttgcagtg aggcatcgcg aacccgtggc cgaatcctgt
      961 ggggtaggcg tcctcacgaa tccaaaccgc ttcctcgatc gcgacgctgt cactgatgcg
     1021 atcatcggcg gcgagcagct ccgccagcat ct
//
LOCUS       DASPLT010000989         2526 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_93054, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000989 DASPLT010000000
VERSION     DASPLT010000989.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 2526)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2526)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2526
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_93054"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1291)
                     /locus_tag="VEA63_10250"
     CDS             complement(<1..1291)
                     /locus_tag="VEA63_10250"
                     /inference="COORDINATES: protein motif:HMM:NF037015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HYD84428.1"
                     /translation="MKFLLASSRMFSGRRLFGGFALALVSCSTPAVAQLYSENFDDGA
                     ADNWLAQSGTWQLSTGSDKTFDSTTAGAGPEIAYYDGASWITGYTYTANVRRQGAGWG
                     NQVGLIFSYQGPNNYYLVTLGGALDPNGKNLHLYERVGGTTTEVASGVFPTTGGGATP
                     NPCDVAIVRSGNVTTVKVNNVALITQTQTALASGGKIGVADQYATGKFDDILVTDSTP
                     PTVSITSPANSATVSNAVNVTATATDDIGVTGVQYQLDGGDLDDETTSPFTLSWNTAL
                     VPNGSHSLTAIARDASGNSTTSAAVNVTVSNTLPSGWTSGDVGTFAPAGSTNYSSGTF
                     LVDGAGSNIWDASDQFHFTRKTVNGDCTVIARVMDVEHTNQYAKAGVMIRESLNANSK
                     YAFIALTPDRPNPADTDRGLLWERRDATGGPAAQNTNI"
     gene            1440..2351
                     /locus_tag="VEA63_10255"
     CDS             1440..2351
                     /locus_tag="VEA63_10255"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HYD84429.1"
                     /translation="MRPAIEIVHPHEGSSLRCFRSTGESFSTIWHFHPELELLLVERS
                     RGMRFVGDSVEPFRPGDLVLVGPNLPHVWINNDLPARPRHDHAMSTLVQFREDFLGRN
                     WTKAAEFAAIAGLLQRSRHGVHFSGADARAAALLMRRLSRTAGVSRVAGLLELLDLLA
                     QSSEQRLLATAGYVPRLNRSDANRIRAVRRYVEKNLGRALRQPKAAALAGLSPARFSA
                     FFRERMGCTFSAYVNRLRLAQVTQLLTTEKMSISEACYASGFNSLSNFNAQFHALKGM
                     SPRAYLRHFRLNAGKGDEWLTRKDWIR"
BASE COUNT          432 a          798 c          812 g          484 t
ORIGIN      
        1 ggatgttggt gttctgcgcc gccgggccgc cggtcgcatc gcgtcgctcc cagagtagac
       61 cgcggtccgt atccgctgga ttgggacgat ccggggtgag cgcgataaac gcatacttcg
      121 aattcgcatt caggctttca cgaatcatca cgcccgcctt cgcgtattgg ttcgtgtgct
      181 cgacgtccat cacccgcgca atgaccgtgc agtcgccgtt gaccgttttc cgggtgaaat
      241 ggaactggtc cgacgcgtcc cagatattgg agcccgcgcc gtcgacgaga aacgtccccg
      301 acgaatagtt cgtgctgcca gccggcgcga acgtgcccac gtcgcccgag gtccagcccg
      361 acgggagcgt gttcgacacc gtgacgttca ccgccgcgga cgtcgtgctg ttgccggagg
      421 catcacgcgc aatcgcggta aggctatggg aaccgttcgg cacgagcgcg gtgttccacg
      481 agagtgtaaa tggcgacgtg gtttcatcat cgagatcgcc accgtcgagt tgatactgca
      541 cgccggtcac accgatgtcg tcggtcgccg ttgcggtcac gttcacggcg ttcgaaacgg
      601 tcgcgctgtt ggccggcgac gtgatcgaga ccgtgggcgg cgtggaatcg gtgacgagga
      661 tatcgtcgaa ctttcccgtg gcatactggt cggcgacgcc gatctttccc ccgctggcca
      721 gggcggtttg cgtctgcgtg atcaaggcga cgttgttgac cttgacggtc gtgacgttgc
      781 cgctgcgcac gatcgcgacg tcgcacggat tcggcgtcgc gcctcctcct gtcgtcggga
      841 aaacgccgct ggccacttcc gtcgtggtgc cgccgacccg ttcgtagaga tgaagatttt
      901 ttccgttcgg atcgagcgca ccgccgagcg tcaccaggta gtagttgttc ggcccctgat
      961 aggagaagat gaggccgacc tggtttcccc aacccgcacc ttggcggcgc acgttcgccg
     1021 tgtaagtgta gccggtgatc cagctcgcac catcgtagta ggcgatttcc gggccggcgc
     1081 ccgccgtcgt gctgtcgaac gttttgtcgg agccggttga aagctgccag gtgcccgact
     1141 gcgccagcca gttgtcggcg gcaccgtcgt cgaagttttc ggagtaaagt tgagcgacgg
     1201 caggcgtgga gcaggaaacc aaagcaaggg cgaagccgcc aaagaggcga cgcccggaaa
     1261 acatgcgcga ggacgcgagg aggaatttca tcggaggatc tttcggggtg aacagcatcg
     1321 ccgccgtcaa ccgcgtccgc gcaggggaca gggcgcgaag ggggcgaatc atgtcccatt
     1381 attgaccaag cctgattttc cactaccgac ctattacgga agagtgccgg tttttttgcg
     1441 tgcgccccgc gatcgaaatt gtccatcccc acgaaggcag ctcgctgcgc tgcttccgct
     1501 cgaccggcga aagcttttcg acgatctggc attttcatcc cgagctcgag ctgctgctcg
     1561 tcgaacgcag ccgcggcatg cggttcgtcg gcgacagcgt cgagccgttc cgccccggcg
     1621 atctcgtgct ggtcgggccg aatctgccgc acgtgtggat caacaacgat cttccggccc
     1681 gcccacgcca cgatcacgcg atgagcacgc tcgtccaatt tcgcgaggat ttcctcggtc
     1741 gcaactggac caaggccgcc gagttcgcgg cgatcgccgg gctgctgcaa cgcagccggc
     1801 acggcgttca tttctcgggc gccgacgctc gcgcggccgc gctcctcatg cgccggttgt
     1861 cgcgcaccgc gggcgtgagt cgcgtcgccg gtttgctcga actgctcgat ctgctcgcgc
     1921 agtcgagcga gcagcggctg ttggccaccg ccggttacgt gccgcggctc aaccgttccg
     1981 acgcgaatcg catccgtgcg gtgcggcgct atgtggagaa aaacctcggt cgcgcgctgc
     2041 gccaaccgaa ggcggcggcg ctcgccggcc tcagcccggc gcggttcagc gcgtttttcc
     2101 gcgaacggat gggctgcacg ttttccgctt acgtgaaccg gctgcgactc gcgcaagtca
     2161 cgcagcttct caccacggag aagatgagca tctcagaagc gtgctacgcc agcgggttca
     2221 acagcctgtc caacttcaat gcgcagttcc acgccttgaa aggcatgagc cctcgcgcct
     2281 acctgcgcca cttccggctg aacgcaggga aaggcgacga atggctcacg cgaaaggatt
     2341 ggattcgctg aaacgacgag gcccgaccgt tgaccggccg ggcctcgtga aagcgacggt
     2401 gttacaccga cgcgatgccg ctcactgctt gagcagcaga aggccgtcgc cggcttggag
     2461 cgtgaacggc ccggtgatcg tcgcgccgtt gttcgtcgtg gaatcctgga taccttgaaa
     2521 gcgctt
//
LOCUS       DASPLT010000990         1109 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_176227, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000990 DASPLT010000000
VERSION     DASPLT010000990.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1109)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1109)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1109
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_176227"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1109
                     /locus_tag="VEA63_10260"
     CDS             <1..>1109
                     /locus_tag="VEA63_10260"
                     /inference="COORDINATES: protein motif:HMM:TIGR02603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HYD84430.1"
                     /translation="NRITLRDADSFEVLKNAPAPRHDTAQQVTWNLSDIAGKAVFLEL
                     VDGDAAGAYAWLAIGRFEPPLLAMPKVTPKLVDERLQAAAQIVLATRDNALESELATA
                     LKSQAGVDARASIAATLLALDAQAHLPVCAGILRDAAAPTLLREKVAQAIADSNSSAG
                     REVLVEVLRGAPERQAPKLALALAGSAAGAEAVLTIAEKGQLSARVLQDRAVKARLVA
                     SKAKDAEARIAKLTKSLTPLNEELQKQIDRRRAAFIPSSASADRGVAVFEKTCIACHQ
                     IEKKGALVGPQLDGIGSRGLERIIEDIVDPNRNVDHAFQTTTFVLSDGDIVSGLLRRE
                     EGESIVYAEATGKENTLPRNSVKERHTSELSLMPE"
BASE COUNT          225 a          370 c          335 g          179 t
ORIGIN      
        1 gaaccgcatc accctccgcg atgcggattc gttcgaggtg ttgaagaacg cgcccgcacc
       61 gcggcatgac accgcgcagc aagtgacctg gaacctatcc gacatcgcag ggaaggcggt
      121 ctttttagaa cttgtcgatg gcgatgcggc cggggcttac gcatggctgg caattggccg
      181 gtttgaaccg ccgcttctcg ccatgccaaa agttacgccg aaactcgtcg atgaacggct
      241 gcaggcggca gcccaaattg ttctcgccac gcgggataac gccctcgaat ccgaactggc
      301 gacggcgttg aaatcgcagg ctggcgtgga cgcacgcgcg agcatcgcag cgaccctgct
      361 cgctttggat gcccaggcgc accttcccgt gtgtgccggg atcctgcgcg atgcggccgc
      421 gcccaccttg ctgcgcgaga aagtcgcgca ggcgatcgcc gactcgaact catccgcagg
      481 acgggaggtt ctcgtcgagg tgttgcgcgg cgccccggaa cgccaggcgc caaagctcgc
      541 tctggcactg gccgggtccg ccgccggggc tgaggccgtc ctgaccatcg ccgagaaagg
      601 ccagctctcc gcgcgcgtcc tccaggatcg cgccgtcaag gcccggctcg tggcttccaa
      661 agccaaagat gccgaagcgc gcatcgcaaa gctgaccaaa agcctcaccc ctttgaacga
      721 ggaattgcaa aagcaaatcg accgccgccg cgctgccttc atcccgtcct ccgcctccgc
      781 ggaccgcggc gttgcggtgt tcgaaaaaac atgcatcgcc tgccaccaaa tcgaaaagaa
      841 gggcgcgctg gtcggcccac agctcgacgg catcggctcg cgcgggctcg agagaatcat
      901 cgaggacatc gtcgatccca atcgcaacgt tgatcacgcc tttcaaacaa cgacgtttgt
      961 tctcagcgac ggcgacatcg tctccggcct gcttcgacgg gaagaaggcg agtccatcgt
     1021 gtacgcggag gccaccggca aagagaatac cctgcccagg aacagcgtga aagagcgtca
     1081 cacgtccgag ctttcgctca tgcccgaag
//
LOCUS       DASPLT010000991         1594 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_197034, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000991 DASPLT010000000
VERSION     DASPLT010000991.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1594)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1594)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1594
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_197034"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..329
                     /locus_tag="VEA63_10265"
     CDS             <1..329
                     /locus_tag="VEA63_10265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD84431.1"
                     /translation="APASREEVPAVVRDAPESTALAIDPVDGRVRVFLKNVKPEAVVT
                     VRMTDGPRVVVEASGAAAKARFRTGSGSLEVTGVEGGTVTVEIPRTVTDGLVEQDGKV
                     IYRTGR"
     gene            481..>1594
                     /locus_tag="VEA63_10270"
     CDS             481..>1594
                     /locus_tag="VEA63_10270"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HYD84432.1"
                     /translation="MLAAFSLALLAAVSLQQGPNATVRGQVRSEGSGAPLKYATVEVV
                     SANRVIQTTTDTLGVYVLRGVPNGRRVLRATHFDHAPLEIEVIVNTTNELVLDFGLEI
                     RPVKLAALTARAVTYKSAGGDTLSVGEAALGQAEVKAMEATPGVAELGLAEATRDVPG
                     QTPTDPTDVLYVRGGTADLKLVLLNGAPVYAPFHLGGLINALDSDFMRAAKLYVGGAP
                     ARYDGGLSYVLDLETRAGRPQQSHGSLAADLLGSRMLMEGPITQTSSYLIGARAVHGM
                     GAQAFLDDPFPYSYTDGLGRVDLGFGGGQLTLTGFWNQEQVRMDSLSGGNARAIWGNT
                     AGSVRYRRRILGSDADLMLAAGDFNTQLPVGGVRPII"
BASE COUNT          271 a          492 c          523 g          308 t
ORIGIN      
        1 ccgcacctgc gtctcgggaa gaagtgcccg ccgtggtgcg cgatgcaccc gagtcgaccg
       61 cgctcgcgat cgatccggtc gatggccggg ttcgcgtttt cttgaagaac gtcaagccgg
      121 aagccgtggt caccgtccgc atgacggatg gtccgcgtgt ggtcgtcgag gcgtccggag
      181 cagccgccaa ggctcgcttc cgtaccggct caggctctct cgaagtcact ggcgtcgaag
      241 gtggcaccgt gacggtcgag atcccgagaa ccgtgaccga tggcctcgtc gaacaagatg
      301 gcaaggtgat ttaccggacg gggcgctagg gcgccccgtt tgtttttatc ccttccccta
      361 tccctccccc tggccccaat cccaagttcc tcgaccttcg tccctcgaaa tccgggaccg
      421 aatgactggt cacccgtgta agtttccgtt acagttggat gatcccttac ccggactgaa
      481 atgctcgctg ctttctccct cgccctgttg gcagccgtgt cgcttcagca aggtccgaat
      541 gcgaccgtgc ggggacaggt gcgcagtgaa ggttcgggcg ctccgctcaa gtacgcgacg
      601 gtagaagtcg tttcggcaaa tcgcgtcatc caaaccacca cagacaccct cggcgtttat
      661 gtactgcgcg gcgttcccaa cggacgccgg gttctgcgtg cgacgcattt cgatcacgca
      721 cccctcgaaa tcgaagtcat cgtcaacacg accaatgagc tggtgctcga tttcggcctc
      781 gagatccggc cggtgaagct ggcggcgctt acggcgcgcg cggtgacgta caagagcgcg
      841 ggcggcgaca ccttgtcggt cggagaggcg gcgttgggac aggcggaagt aaaggcgatg
      901 gaggcgacgc ccggcgtcgc tgagctggga ctggcggaag caacgcgcga tgtgccgggg
      961 caaacgccaa cggacccgac cgacgtgctg tacgtgcgcg gcggcaccgc cgatttgaag
     1021 ctcgtgcttc tgaatggcgc gcccgtgtat gcgccattcc atctcggcgg cttgatcaac
     1081 gcgctggatt ccgattttat gcgcgcggcc aagttgtacg tgggtggcgc gcccgctcgc
     1141 tacgacggcg gactctccta cgtgctcgat ctcgaaacgc gcgcggggcg cccgcagcaa
     1201 agtcatggat cgctcgctgc ggatctgctg gggtcgcgca tgttgatgga aggtccgatc
     1261 acgcaaacgt cgtcgtatct gatcggcgcg cgcgcggtgc acggcatggg cgcgcaagca
     1321 tttctcgacg atccgttccc atacagctac accgacgggc ttgggcgcgt cgatctgggt
     1381 tttggtggcg ggcagctgac gctcaccggc ttctggaatc aggagcaggt gcgcatggat
     1441 agcctttcgg gaggtaatgc ccgcgccatc tggggcaata ccgccggatc ggtgcgctat
     1501 cgccggcgca tactgggatc cgatgcggat ttgatgctgg cggcaggcga cttcaacacg
     1561 cagctgccgg taggcggtgt gcggccgatc atca
//
LOCUS       DASPLT010000992         2051 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_113483, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000992 DASPLT010000000
VERSION     DASPLT010000992.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 2051)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2051)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2051
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_113483"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            165..713
                     /locus_tag="VEA63_10275"
     CDS             165..713
                     /locus_tag="VEA63_10275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012372865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HYD84433.1"
                     /translation="MPKTTTLQELLVEELKDLYSAETQLTKALPKMAKAASDENLKAG
                     FEEHLDQTHEHIARLDRAMELLDASPKGKTCKAMKGLIAEGEEKIKEDASPAVRDAAL
                     IGAAQKVEHYEIAGYGTVRTFAELLGEDEVVSLLQETLDEEAETDRKLTELASSLNLK
                     AESGEDEDEDESDEEEEDEEEK"
     gene            1042..1344
                     /locus_tag="VEA63_10280"
     CDS             1042..1344
                     /locus_tag="VEA63_10280"
                     /inference="COORDINATES: protein motif:HMM:NF022603.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2934 domain-containing protein"
                     /protein_id="HYD84434.1"
                     /translation="MNENTSTLSNTNRLSHDNISRKAKELWESYGRPEGRDEEIWFEA
                     ERSLSASMGNIENAGEQRPVDTPMKNSATATGNSAVASGAPGGRGAQPRGAKAGKR"
     gene            1548..>2051
                     /locus_tag="VEA63_10285"
     CDS             1548..>2051
                     /locus_tag="VEA63_10285"
                     /inference="COORDINATES: protein motif:HMM:NF024884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4118 domain-containing protein"
                     /protein_id="HYD84435.1"
                     /translation="MLALCGAVYARQGLSSWLGETPFAFLLPAVFVSAWFGGTWVGVG
                     ATLVGVLIAAAGFPEPFVFNHPSEWTRLLLFTTNAAFATGICAILHHVVHTSRASANE
                     AARNFEIMANNAPVLIWSTDADGRCRFVNHNWLAFTGRPLERGSEARRPGQLHPGDAG
                     RYETVSQE"
BASE COUNT          429 a          658 c          599 g          365 t
ORIGIN      
        1 cgcgtttcgg gatttccccg ggacggaaac ggcggcgtca cgcggaactc ccgcagcgcg
       61 cacgccacca ttcgacgcga cgaccgctcc ggaaattcag ccgccttccc ggcgcgctgc
      121 cggtccgttc gtcgcgacaa cccaaacctc aaccattcca tcccatgcct aaaacaacca
      181 ctctccagga actcctcgtt gaagaactca aggaccttta cagcgccgag acccagctca
      241 cgaaagcgct gccaaagatg gccaaagccg cctccgacga aaatctcaag gccggcttcg
      301 aagaacacct cgatcaaacg cacgagcaca tcgcccgatt ggatcgcgcg atggaattgc
      361 tcgatgcctc gcccaaaggc aaaacctgca aagcgatgaa gggcctcatc gccgagggtg
      421 aagagaagat caaggaagac gcctcacccg ccgtgcgcga tgccgcgctc atcggcgccg
      481 cgcagaaagt cgaacactac gagatcgccg gctacggcac cgtgcgcacg tttgccgaat
      541 tgctcggcga ggatgaagtc gtatcgcttc tccaggaaac gctcgacgaa gaggccgaaa
      601 ccgatcgcaa gctcaccgaa ctcgcttcgt ctctcaacct caaggccgag agcggcgaag
      661 acgaggacga ggacgagagc gacgaagaag aagaggacga agaagaaaag tgaccggcgc
      721 cacgccgtcg cgttttcgct tcccgaccgc ttctcgctga cgcggatgtc gggtgagtga
      781 agatgttgaa gataggggcg tcgctcgtcg gcgccccgaa ttgcgagcga agcggcacgg
      841 acgcaaggtc cggccctaca ctcaatcgtg acagcgcccg cgaataaata ttcgccctgg
      901 caaccttgct cagagttagc acaaacggct ccgccaaccg cggagccgtt tgtcgttttc
      961 cttgcccggt tttttgcggc ggagtccagc cggaatcccc ctgattccac catcgcgcaa
     1021 ctgatgctat tttggcggaa catgaacgaa aatacctcca ccctttcgaa caccaaccgg
     1081 ctgtctcacg acaatatttc ccgcaaagcg aaagaacttt gggaaagcta tggccgccca
     1141 gaaggccgcg acgaagaaat ttggttcgaa gccgaacgca gtctttccgc ctcgatgggg
     1201 aacatcgaaa acgccggcga gcagcggccc gtggatacgc cgatgaaaaa ctctgcgaca
     1261 gccactggaa attccgccgt cgcctccggc gcgcccggcg ggcggggcgc acagccgcgt
     1321 ggcgccaagg ccggcaaaag atagcctgac ttggtgcaag agtttggcgc gtcaagtcac
     1381 gctggccctg atgtgcggtt aaggtttttc ccgatgggtt gattcggtga caaatacgag
     1441 ccggagcgca ctatacgcgc gtgtggttcc agccccgcat cttttctcgt ccgcgagttt
     1501 tggtttctcc gcgcgagcgt agcgttgtca cgcgctacgg cgttgccgtg ttggctctct
     1561 gcggagcggt ttatgcgcgg caaggattga gctcgtggct gggcgagacc cccttcgctt
     1621 ttttgttgcc ggccgtgttt gtcagcgcgt ggttcggcgg cacttgggta ggcgtcggcg
     1681 ccacgctggt cggggtgtta atcgccgcag ccgggttccc ggaaccgttc gtcttcaacc
     1741 atccgtcgga gtggacgcgg ctgctgcttt tcacgaccaa cgccgctttc gcgacaggaa
     1801 tttgtgcgat tctccaccat gtcgtgcaca cctcgcgcgc gtccgccaac gaagccgcac
     1861 gcaatttcga gatcatggcg aacaacgcgc cggtgctcat ctggtccacc gacgccgatg
     1921 gccgctgccg cttcgtgaac cacaactggc tcgccttcac cggtcggccg ttggaacgcg
     1981 gatccgaagc ccgccggccc ggccagcttc accccgggga cgccggccgc tacgaaacgg
     2041 tttcccaaga a
//
LOCUS       DASPLT010000993         2502 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_277499, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000993 DASPLT010000000
VERSION     DASPLT010000993.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 2502)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2502)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2502
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_277499"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..228
                     /locus_tag="VEA63_10290"
     CDS             <1..228
                     /locus_tag="VEA63_10290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD84436.1"
                     /translation="RRNVWASGIGYAMFVWTVMNYIVLPSSALSAKLPNFASVHTYIG
                     ACVLVLVFGLPIAFGAARFYNSQRFSAKVEP"
     gene            348..1232
                     /locus_tag="VEA63_10295"
     CDS             348..1232
                     /locus_tag="VEA63_10295"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HYD84437.1"
                     /translation="MNLFTPNYRVSSLCAQIARFCTPLLLAVAGASSAVAAEGPKPPA
                     HVWPNGAACAVSLSYDDAVPVHHQRVAPLLEQHGLRGTFYLLVRAIDNPEAWKQVAAH
                     GHELGNHSLFHPCRRDASNAGWLAEHYDLADYTPGRFRDELAVANLVLDLLDGGKPRT
                     YGNNCTHLTIGRGAREEPMDPILAPMFVAARGTITNQSVDPARPVFTRLGHFSGDNKT
                     FAQLRAEIEAARAQGGWIIYMFHGVGAGTHALFVDDNEHRQLIEWLDQERGSIWTAPV
                     VDVARHLQAANGSAAAAP"
     gene            1351..>2502
                     /locus_tag="VEA63_10300"
     CDS             1351..>2502
                     /locus_tag="VEA63_10300"
                     /inference="COORDINATES: protein motif:HMM:NF012958.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HYD84438.1"
                     /translation="MKLHPLPHLALVVAGVLNASLFAADIAPSNVPPPAAAARPQPPT
                     RSPDAPGAPRFVRLDGKAGANPPVDAVGNFVIGPNYVPAPELKVVEGVPQGKVTQFTM
                     RSKDSKFFNPGIARDAFGTVDPNNPKTLIVETHPIDYERTITVYVPAHYTSGTPVPFI
                     VTHDGPPLGKPNLDLSHVLDNLIAQNRVPAQVAIMIQHGGGDAQGHERGREYDTMSGT
                     FAEFIEAEVLPLVEKNCGVTLTKDPEARAAMGNSSGGAAALIMAWYHPELYRRVITTS
                     GTFVNQQWPFNPETPGGAWDFHAKLIPDSPRKPIRIWMGVGDRDLLNPNVMRDNMHDW
                     VEANHRMAHVLAAKGYRYQYVFALDVGHSMGAARAQTLAHALEWVWHDYP"
BASE COUNT          449 a          799 c          796 g          458 t
ORIGIN      
        1 cgccggaacg tttgggcgtc cggaatcggg tatgcgatgt tcgtttggac ggtgatgaac
       61 tatatcgtgc tgccatccag cgcgctgtcc gcgaagctgc cgaacttcgc aagcgtgcac
      121 acgtatatcg gcgcgtgcgt gctggtgctc gtgttcgggc tgccgatcgc gttcggtgcc
      181 gcgcgcttct acaattcgca acggttctcg gcgaaagtgg agccgtgagg cggaagcctt
      241 aactttcgac agcgggccgg tcgaagccca tggcgtgact cgaacaatct ttgacgcgcg
      301 cggcagcgga gcccaaagaa tccaagtcga agctaactgc atcgactatg aatctcttta
      361 cgccgaatta tcgtgtttca tcgctgtgcg ctcagatcgc gcgcttttgc acgccgctcc
      421 tgctggctgt cgcgggtgcg tcgagcgcgg tggccgccga aggcccgaag ccgccagcgc
      481 atgtctggcc gaatggggcg gcgtgcgcgg tttcgttgag ttacgacgat gcggttcccg
      541 tgcatcacca gcgagtcgca ccgctgcttg aacagcacgg gctgcgcggc acgttctacc
      601 tcctcgttcg ggccatcgat aatcccgagg cttggaaaca ggtcgcggcg cacggtcacg
      661 aactgggcaa tcattcgctc tttcatccct gccgtcgcga cgccagcaac gccggctggc
      721 tggcggaaca ttacgatctc gcggactaca cgcctgggcg tttccgcgat gagctcgcgg
      781 tggcgaatct cgtgctcgac ctgctcgatg gcggaaaacc gcgcacctac ggcaacaact
      841 gcacccacct gacgatcggt cgcggcgcgc gcgaggagcc gatggatccg attctcgcgc
      901 cgatgttcgt ggccgctcgc ggcacgatca ccaatcagtc cgtcgatcct gcgcggcccg
      961 tattcacgcg gctgggacac ttcagcggcg acaataaaac gtttgcgcag ctccgcgcgg
     1021 aaatcgaagc ggcgcgcgct cagggcggat ggatcatcta catgtttcac ggcgttggcg
     1081 ccggcacgca tgccctgttc gttgatgaca acgaacatcg tcaactgatc gaatggctcg
     1141 atcaggagcg cggatcgatt tggaccgcgc ccgtcgttga cgtggcgcgt cacctccaag
     1201 ctgcgaatgg aagcgcagct gccgcgccgt agctgccgac cggtcgcgca acagcaagcg
     1261 ggtgcggggc cggaagcatt catctctggc attgaaggcg gtcggcgtgg cgtagagcgt
     1321 gaggtgcttc cccgccgctc ttcgccgacc atgaaactcc accccttgcc ccacctcgcg
     1381 ctcgttgttg ccggtgtgct caacgcgtcg ctcttcgccg ccgatatcgc gccttccaac
     1441 gtgccgccgc ccgcggccgc cgctcgtccg caaccgccca cgcgttcgcc cgacgcgcca
     1501 ggcgcgccga ggtttgtccg cctcgatggc aaagcaggcg cgaacccgcc ggtcgacgcc
     1561 gtcggaaatt tcgtcatcgg gccaaactat gtgccggcgc ctgagttgaa ggtcgtcgag
     1621 ggtgtgccgc aagggaaggt gacgcagttc acgatgcgtt cgaaggacag caagtttttc
     1681 aaccctggca tcgcgcgcga cgcctttggc accgttgacc cgaacaatcc aaagacgctc
     1741 atcgtcgaaa cacacccgat cgactatgag cgaacgatca cggtgtatgt gccggctcac
     1801 tacacgtccg gcacgccggt gccgttcatc gtgacgcacg atggcccgcc gttggggaag
     1861 ccgaatctcg atctgtccca cgtgctcgac aatctgatcg cgcagaaccg cgtgccggcg
     1921 caggtcgcga tcatgattca gcatggaggc ggcgatgcac agggccacga acgcggacgc
     1981 gaatacgaca cgatgtccgg cacgtttgcc gaattcatcg aggcggaggt gctgccgctc
     2041 gtggagaaaa actgcggcgt cacgttgacg aaggatcccg aggcgcgcgc cgcgatgggc
     2101 aacagttcgg gtggcgccgc ggcgttgatc atggcgtggt atcacccgga actctaccgc
     2161 cgcgtgatca cgacctcggg cacgttcgtc aaccagcagt ggcctttcaa cccggagacg
     2221 ccgggcggcg cgtgggattt tcacgcgaag ctgattccgg acagcccgcg caagccgatc
     2281 cggatctgga tgggcgtggg cgatcgcgat ctgctcaacc caaacgtcat gcgcgacaac
     2341 atgcacgact gggtcgaggc gaaccaccgg atggcgcacg tgctcgcggc gaaaggctat
     2401 cgttaccaat acgtgttcgc tctcgatgtc ggccacagca tgggagccgc gcgggcgcag
     2461 acccttgccc acgcgctcga gtgggtgtgg cacgattatc cg
//
LOCUS       DASPLT010000994         1316 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_135225, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000994 DASPLT010000000
VERSION     DASPLT010000994.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1316)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1316)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1316
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_135225"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..191)
                     /locus_tag="VEA63_10305"
     CDS             complement(<1..191)
                     /locus_tag="VEA63_10305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HYD84439.1"
                     /translation="MKDQEADVDSREDPYNVTQASRIYFLPNAMTAGNLFCGFMAVVS
                     CVHARLAETALDGEYLAATP"
     gene            complement(188..538)
                     /locus_tag="VEA63_10310"
     CDS             complement(188..538)
                     /locus_tag="VEA63_10310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytotoxic translational repressor of
                     toxin-antitoxin stability system"
                     /protein_id="HYD84440.1"
                     /translation="MYQVTFSEQSMRELNQLDKLAQLDVIGPISSLRPADLAHPREPL
                     GRFHRGERQYYRLRAGDYRLYFEVLGDTLHTHYILHRNSLEDFLLRNKLPVTEQQLVE
                     QHSKFWKYLESLTK"
     gene            987..>1316
                     /locus_tag="VEA63_10315"
     CDS             987..>1316
                     /locus_tag="VEA63_10315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD84441.1"
                     /translation="MSSIPVFADAHSRLHADIAIIRLIRAGIRTGRISAVFPSRRAPN
                     SVCCWLKDFRRIALRSALPIATAGLLGQLFNRKARRADIENTLESLGLAQEMAERMLE
                     KVRDGQIV"
BASE COUNT          258 a          403 c          387 g          268 t
ORIGIN      
        1 ggcgtcgcgg cgaggtattc gccgtcgagc gcggtttcgg cgagtcgtgc gtgaacgcag
       61 ctcacgacag ccatgaagcc gcagaagaga tttcccgctg tcatcgcatt cggcaggaaa
      121 taaatccggc tcgcctgcgt gacgttgtag ggatcctcgc gcgaatcgac gtcggcttcc
      181 tgatccttca tttcgtgagg ctttcgaggt atttccaaaa cttggaatgt tgctcgacga
      241 gttgttgttc ggtgaccggg agcttgttgc gcagcaggaa atcctcgagc gagttgcggt
      301 gcagaatgta gtgcgtgtgc agcgtgtcgc ccaaaacctc gaagtagagg cggtagtcgc
      361 ccgcgcgcaa acgatagtat tgccgctcgc cgcgatgaaa acggccgagc ggttcacgcg
      421 ggtgagccag atccgcggga cgcaggctgc tgatcgggcc gatgacgtcg agctgcgcga
      481 gcttatcgag ctggttcagc tcgcgcatgc tttgttcgga gaaagtgacc tgatacatcg
      541 tggtgattcc gacagataga gagcgggatt aagtcctcgg gttgccctcg caggatcggc
      601 acgaggtgca atttctcagc gcggcgccga aggcgagggt tcttacgccc ggcgtgcggt
      661 tcatcaatgc aattcccgtt gcgcctgcga gcaggtggaa cccgacccgt cgagttgatc
      721 ggcccgcgct aattcacatc gcaacgcaca gcgatctcac gagacgccgt cctccacccc
      781 atgcactctg cacggcaacg cgaacgcttt tcgcaatttg cagcgtaaaa ccgccacgcg
      841 cagttattca caaatcgacg atagatgcgc gcacactagc tatttacgcc cgctggcttc
      901 gtctggaaca agcctagcta agcatcgggc caacgcttca aagaacccgc cacgccagat
      961 ttaaccactc aacgcttctt ctccccatgt cctccatccc tgtttttgcc gacgctcatt
     1021 cacggctcca cgccgatatc gccatcatcc gcctgatccg cgccggtatt cgcaccggga
     1081 gaatctcggc cgtattcccg tcccgccggg cgcccaacag cgtgtgctgc tggctgaagg
     1141 attttcgtcg catcgccctg cgctccgcgc tgccgatcgc gacagcaggg ctgctaggcc
     1201 agttgttcaa ccgcaaggct cgtcgcgccg acatcgaaaa taccctcgaa tcgctcggct
     1261 tggcgcagga aatggcggag cggatgctcg agaaggtgcg cgacggccag atcgtc
//
LOCUS       DASPLT010000995         1406 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_135247, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000995 DASPLT010000000
VERSION     DASPLT010000995.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1406)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1406)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1406
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_135247"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            188..1201
                     /locus_tag="VEA63_10320"
     CDS             188..1201
                     /locus_tag="VEA63_10320"
                     /inference="COORDINATES: protein motif:HMM:NF012742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HYD84442.1"
                     /translation="MSIPDFAKYLGLSSWTVSRAINGHPEVSEKTRRRIMAAMEEVGF
                     RPNPLARGLGGRRTSMIGVCFMGLGNPILDRKVYHLQEFLRQHQLRSLLEMRMRDVQQ
                     EIRAIEDFRRIHVDGIVLIHSELDAAASRRVLEGAACVHVDPHLPQSTASVSVDRRRA
                     MRLLLEHLLRLGHRTFALLGITEADPWRWSAVVETVQAHGLDPARALRGVEQPSEVES
                     PIEAGELMAEKVLAWPKRPTALIALDDRRALGAIQALKNANIEVPRQMSVTGFDNLDF
                     ARKLHPTLTSIEQNPLRLMERAGAMLLEQLELTAEERGRGVSETVTPDLVIGESTGPA
                     WRG"
BASE COUNT          229 a          409 c          502 g          266 t
ORIGIN      
        1 cgcatcaacg gcgcgctaaa agtgacgatt aggctgcacg gccccggaag tttccttgct
       61 atcgaatcga cgcgctcaat tctcgcggcg gcccgaattc cgggttttcc agcacccctc
      121 gtagaatatg gcgcgcagcg aatcctcttc gaagaaagcg acccgtcggc cggcgtccgg
      181 accgatcatg tcgattcccg actttgcgaa gtatctaggc ttgtccagtt ggacggtctc
      241 gcgggcgatc aacggccatc cggaagtgag tgagaaaacc cggcgacgga tcatggcggc
      301 gatggaggag gtcgggtttc ggccgaatcc gctcgcgcgc ggactgggcg gccgccgcac
      361 gagcatgatc ggtgtctgtt tcatggggct gggcaatccg atcctcgatc ggaaggttta
      421 tcatcttcag gagttcctgc gtcagcatca gctccgcagt ctgctcgaga tgcgcatgcg
      481 cgacgtgcag caggagattc gtgcgatcga ggattttcgc cgcattcatg tcgacggcat
      541 cgtgctcatc cattccgagt tggacgccgc ggcttcgcgg cgggtgctgg aaggcgccgc
      601 atgcgtgcat gtggatcctc atctgccgca gtccaccgcg agcgtgtcgg tcgaccggcg
      661 tcgcgcgatg cgccttttgt tggagcattt gctgcggctt gggcatcgga cgtttgcgct
      721 gctcggcatc accgaagccg atccgtggcg ttggtcggcg gtggtggaaa cggtgcaggc
      781 gcacgggctg gatccggcgc gggcgttgcg gggcgtcgag cagccgtcgg aggttgagag
      841 cccgatcgag gccggcgagt tgatggcgga gaaagtgctc gcgtggccga agcgcccgac
      901 cgcactgatc gcgctcgatg accggcgggc gctcggcgcg attcaggcgt tgaagaacgc
      961 caacatcgag gtgccgcggc agatgtcggt gacgggattc gataatctcg atttcgcgcg
     1021 caagctccat ccgacgctca cctcgatcga gcagaatcca ttgcgcttga tggagcgggc
     1081 gggcgcgatg ctgctggagc aattggagct aacggcggag gagcgcgggc gtggcgtcag
     1141 cgagacggtc acgccggatc tggtgatcgg tgaatcgacg gggccggctt ggcgcggtta
     1201 acgcgatcgc acgcggatga ctcgacgcgt ccaccgcggc gcgctctgat tgcgagcgcg
     1261 ccgggcggac ccgagggcta cttcgatgcg ggcatcaatt cgttggccgt ccacatttcg
     1321 cggcgctgac ggtagtcgcg gcgcgcggcg aggacgtcgg cttcggcgat cggcgcggcg
     1381 tcgttgccga agctttggcg cacgta
//
LOCUS       DASPLT010000996         1017 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_35489, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000996 DASPLT010000000
VERSION     DASPLT010000996.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1017)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1017)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1017
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_35489"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..185
                     /locus_tag="VEA63_10325"
     CDS             <1..185
                     /locus_tag="VEA63_10325"
                     /inference="COORDINATES: protein motif:HMM:NF016883.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016756 - glutathione
                     gamma-glutamylcysteinyltransferase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0010038 - response to metal ion [Evidence
                     IEA];
                     GO_process: GO:0046938 - phytochelatin biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phytochelatin synthase family protein"
                     /protein_id="HYD84443.1"
                     /translation="GHGHISPILGYLPEEDLVFVGDVNARFRPFLVPSERLLEAMNTT
                     DTSTGRSRGLLRIGPA"
     gene            complement(621..>1017)
                     /locus_tag="VEA63_10330"
     CDS             complement(621..>1017)
                     /locus_tag="VEA63_10330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007359214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HYD84444.1"
                     /translation="LVAGAAAPKLITRDMLKTMRPGSVLVDISIDQGGCFETSKATTH
                     SEPTFVVDGIVHYCVANMPGAVSRTSAFALNNATLPFTLALANKGYKKALQDDPHLAN
                     GLNVYEGAITHEAVARDLGKTYKKPGWLG"
BASE COUNT          207 a          339 c          284 g          187 t
ORIGIN      
        1 ggggccacgg ccacatctcg ccgatcctcg gctacttgcc ggaagaggat ctcgttttcg
       61 tcggcgacgt gaacgcgcgc ttccgaccgt tcctggtccc gagcgaacgc ctgctcgaag
      121 cgatgaacac gaccgacacc agcaccggcc gcagccgcgg cctgcttcgc atcggcccgg
      181 cttgaaaaat ggcgcttggg ccggccactc acgccgccct cgctttgctc cagccggccg
      241 tcagcgaaac tcgcgtaaga ttgcgaaggc ccagatagag aagatgagcg caaggaggca
      301 aagcgtcggg ctttagcgac gaggtcatct ggaagctcat gcttgcaagc atcgatcgca
      361 agccaaaccg tcgcgttgaa cggatgatag ccaaatacgc ttggtcgccc tttcggccat
      421 gggttgaacc cgacaaacgg acttacaact gccatttctc gccgaagctc aggcatccgc
      481 tcgcgcacgc tctcttcgac cggggtgccg gcgatcgctc gcattacccg gctgcaaacc
      541 gtcgcaccaa aaatatggag ggctgcaaaa gtcacaacga gcgctgcgat tgcaccccaa
      601 tagaccatag tctcgaggac ctatcccagc cagccgggct tcttgtaggt cttgcccaaa
      661 tcccgcgcca ccgcttcgtg cgtgatcgcg ccttcgtaga cgttgagccc gttcgccaaa
      721 tgcggatcgt cttgcagcgc ttttttgtag cccttgttcg ccaacgccag cgtgaacggc
      781 agcgtcgcat tgttcaaagc aaacgctgac gtgcgcgaga ccgcccccgg catgttggcg
      841 acgcaatagt gcacgatgcc gtccacaacg aacgtgggct cgctatgcgt cgtcgctttc
      901 gacgtctcaa agcagccgcc ctgatcgatc gaaatatcga ccagcaccga gcccggccgc
      961 atcgtcttca gcatatcgcg cgtgatcagc ttcggcgccg cagcgcctgc aacgagc
//
LOCUS       DASPLT010000997         1135 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_176325, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000997 DASPLT010000000
VERSION     DASPLT010000997.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1135)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1135)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1135
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_176325"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            35..>1135
                     /locus_tag="VEA63_10335"
     CDS             35..>1135
                     /locus_tag="VEA63_10335"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HYD84445.1"
                     /translation="MAVLIAIGWLGWRAFAARNIAIASIPARPDLSGWSPILAARLQE
                     AEESARGFGNATAGVAELSRLYHANGFLLEASQCYNGLRELESSNARWPHLLASIVAG
                     YGRLDEAKPLLETALRLAPDYIPARLRLAEALLKSNDLIGAAAAYADVLRREPGNPYA
                     ELGLARCSIENGDWTGARERLQKAVTGRPDTFGGWALLVAIHEHFGDREALAAARPRA
                     AAAGRFREMPDPWLDELTEDCYDPYRLSVAAALTTDSARARRWIERAIELSPDTGSYH
                     RQLAILLQRTRDYPAARRHFERAAALNPADSDAWAHLVGLLKDMRNPAAADQALANGL
                     AHCPESPALRHLNGQRLRLLGHFDAARAELELA"
BASE COUNT          177 a          403 c          345 g          210 t
ORIGIN      
        1 gcgccacgct ccgctcgtgc tcacgcttgc cggcgtggcc gttctcatcg cgatcggctg
       61 gcttgggtgg cgtgcgttcg ccgcacgaaa cattgccatc gcttccatcc ccgcgcgtcc
      121 ggatctgagc ggctggtcgc ccatcttggc cgcgcgcctc caggaagccg aggaaagcgc
      181 gcgcggattt ggcaacgcga ccgccggcgt ggccgagctg agccgcttgt atcacgccaa
      241 tggatttctg cttgaagcga gccagtgcta caacggcctg agagagctcg agtccagcaa
      301 cgcccgctgg ccgcatttgc tcgcgagcat cgttgccggc tacgggcgtc tcgatgaagc
      361 aaaaccgctg ctcgaaaccg cgctccgcct cgcccccgat tacatccccg cgcggctgcg
      421 tctcgccgag gccttgctta aatcgaacga tttgatcggc gctgccgcgg cctatgccga
      481 cgtgcttcgc cgcgaacccg gtaatccgta tgctgaactc ggtctcgcgc gttgcagcat
      541 tgaaaacggc gactggaccg gagcgcgcga acgtctccag aaagccgtga cagggcgccc
      601 cgacaccttc ggcggatggg cgctgctggt tgcgattcat gaacatttcg gcgaccggga
      661 ggctcttgcg gccgcgcggc cgcgagcggc cgctgccggt cgttttcgtg aaatgccgga
      721 tccatggctc gatgagctta ccgaggattg ctacgatccc taccgtctct ccgtcgccgc
      781 tgcgttgacg accgactcag cacgggcccg tcgctggatc gaacgcgcga tcgagctttc
      841 gcctgacacg ggatcttacc atcgtcagct cgcgattctt ctgcaacgga cgcgcgacta
      901 tcccgccgcg cgtcggcatt tcgaacgcgc cgccgccctc aatcctgccg actcggatgc
      961 gtgggcccat ctcgtcgggt tgttgaaaga catgcggaat cccgccgccg cagaccaggc
     1021 gctcgccaac ggtctcgccc actgtcccga atcaccggct ctgcgccatc tcaatggcca
     1081 gcgcctccga ctgctcggcc atttcgacgc cgcacgcgcg gagctcgagc tcgcc
//
LOCUS       DASPLT010000998         1784 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_176349, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000998 DASPLT010000000
VERSION     DASPLT010000998.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1784)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1784)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1784
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_176349"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1104)
                     /locus_tag="VEA63_10340"
     CDS             complement(<1..1104)
                     /locus_tag="VEA63_10340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative Na+/H+ antiporter"
                     /protein_id="HYD84446.1"
                     /translation="MVARRSNAVRWVGLVGLATALASLTHAAVPEAGGFPLPLEAYGD
                     TGSGVIETLVARARMEPFNLVATAIFFLAIVHTFLTAKFRHWAHVVEEKHAVQLKERT
                     PVRGDRDGDNAPDEVSFKGQILHFLGEVEAVFGIWVVVLMGAITWLKGWHTVVDYIGH
                     RVNFTEPMFVVVIMALASTRPVLVFAEQCMRAVASLGKQSTGAWWLSILIIAPVLGSF
                     ITEPAAMTIGALLLARQFYELKPSPRFCYATLGLLFVNVSVGGTLTHFAAPPVLMVAA
                     PWGWDMVYMATHFGWRAMVGIVAATGLYYAIFRREFTALDAARRAQRAADVGNEDSGR
                     GPIPAWITVVQLGFMALTVFVAHFPALFVGGFLF"
     gene            complement(1225..>1784)
                     /locus_tag="VEA63_10345"
     CDS             complement(1225..>1784)
                     /locus_tag="VEA63_10345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007362726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1015 family protein"
                     /protein_id="HYD84447.1"
                     /translation="RRERNPQHTGNEDYNWFLCVLFPASELKILPYNRIVFDLNGRTP
                     EAFLAEVKARFGLVENASPTPASVGEVSMYLGGKWYGLRCAADPKADPVARLDVSVLQ
                     DKLLAPLLGIDDPRTSKRVDFVGGIRGTGELVKRVDADGGVAFSLYPVTIAQLMDIAD
                     AGQIMPPKSTWFEPKLRSGLFVHTF"
BASE COUNT          382 a          686 c          448 g          268 t
ORIGIN      
        1 aaacaaaaag cctccgacaa acagcgccgg aaaatgcgcc acgaacaccg tcaacgccat
       61 gaacccaagc tgcaccaccg tgatccacgc cggaatcggt ccgcgtccgc tgtcctcgtt
      121 gccgacatcc gccgcccgct gcgcccgccg cgcggcatcc agtgccgtga actcgcgccg
      181 aaaaatcgca tagtaaagcc ccgttgccgc aacgatgccg accattgcgc gccagccaaa
      241 atgcgtggcc atgtaaacca tgtcccagcc ccacggcgcc gcgaccatca gcaccggcgg
      301 tgccgcgaag tgcgtcaacg ttccacccac cgacacgttc acgaacaaca gcccgagcgt
      361 cgcgtagcag aaacgtggag acggtttcag ttcgtagaac tgccgcgcca gcaacaacgc
      421 gccaatcgtc atcgccgccg gctccgtgat gaacgagccc agcaccggcg cgatgatcaa
      481 aatcgaaagc caccacgcgc ccgtcgactg ctttcccagc gaagcgaccg cgcgcatgca
      541 ttgctccgcg aacaccagca ccggccgcgt cgacgccagc gccatgatca cgaccacgaa
      601 catcggctcc gtgaaattca cgcgatgccc gatgtaatcg acgaccgtat gccagccctt
      661 gagccacgtg atcgcgccca tcaataccac cacccagatc ccgaacaccg cctccacctc
      721 tccgaggaaa tgcaggatct gccctttaaa actcacctcg tcgggcgcgt tgtctccgtc
      781 gcgatcgccc cgcaccggag ttcgctcctt caactgcacg gcatgcttct cctccacaac
      841 atgcgcccag tgacgaaact tggcggtcaa aaacgtgtgc acaatcgcga ggaaaaaaat
      901 cgccgtcgcc acgaggttga acggctccat ccgcgctcgc gccacgagcg tctcgatcac
      961 ccccgagccg gtgtcgccat acgcctccaa cggcagcgga aagccgcccg cttcgggaac
     1021 cgctgcatgc gtcaaactcg ccagagccgt ggcgagtccg accagtccta cccagcgcac
     1081 tgcgttcgat cgtcgagcaa ccatggccgt aggcaaccgc cactcatgcg catggcaagc
     1141 tcacgcgcac gccgacgtag cggcaggcct ccgtgcctgc ctgccctccc cgtccgccac
     1201 tcgctcaccc gccgccgcga cgcgtcaaaa cgtatgcaca aacaaacccg agcgcagctt
     1261 cggctcgaac cacgtgctct tcggcggcat gatctgcccc gcgtccgcga tgtccatcaa
     1321 ctgggcgatc gtcactggat acagcgaaaa cgccacgccg ccgtccgcgt cgacccgctt
     1381 cacgagctca cctgtcccgc gaatcccgcc gacaaaatcc acgcgcttgc tcgtgcgcgg
     1441 atcgtcgatc cccaacaacg gcgcgagcag cttgtcctgc aacacactca catcgagccg
     1501 cgccaccgga tccgccttcg ggtccgccgc gcaacgcagc ccataccatt ttccgccgag
     1561 atacatgctc acttcgccga ccgatgccgg cgtcggcgac gcattttcca ccagtccgaa
     1621 ccgcgccttc acctccgcga gaaacgcctc cggcgtgcgc ccgttcaaat cgaacacgat
     1681 ccggttatac ggcagaatct tcagctcact cgcgggaaac aacacgcaca gaaaccagtt
     1741 gtagtcctcg ttgcccgtgt gctgcggatt ccgctcgcgc cgca
//
LOCUS       DASPLT010000999         1287 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_277585, whole
            genome shotgun sequence.
ACCESSION   DASPLT010000999 DASPLT010000000
VERSION     DASPLT010000999.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1287)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1287)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1287
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_277585"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1287)
                     /locus_tag="VEA63_10350"
     CDS             complement(<1..>1287)
                     /locus_tag="VEA63_10350"
                     /inference="COORDINATES: protein motif:HMM:NF020700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HYD84448.1"
                     /translation="RSLAAGENTFTVQAKDAAGNTAFKSITVRRDTTAPNIAFITPAN
                     GSATKAAVIRVTGTVDDPTAAVLVNGRTATVADGQFEIPDFSLEEGQTFLTAVATDGL
                     GNASAPVSITVTSDRTPPPPAIATPPTYIKTDRFTLSGTAEPGSTVSIIGGLVSVSAT
                     VNTSGEFSALVLVTPNKATDIFIRVVDRVGNESDPVVHTVISDTIAPVITLSRPAAAA
                     SIGTSIVDVVGAVTDANPASSLTVNGQTVALLPNGQFGCRLTLADGAQSIVVAVLDLA
                     GNQAQQTRAITVVNASGDDEPPSIVVLSPSYDAVVPTALIVVTALMTDESPLTSLTAA
                     GSPIIDSAGDGLVTFEVTVDANGEFVLVATDAQNLTTTLTHRVRVDGSTPTEPVILRV
                     SPDGPTAERQLVLYGAGQAGLRYAVGGGLLPTVTGT"
BASE COUNT          256 a          368 c          428 g          235 t
ORIGIN      
        1 tggtcccggt tacggttgga agcaagccgc cgcccactgc gtaacggagc ccggcttggc
       61 cggcgccata gagaacgagc tggcgctcgg cggtcgggcc atcggggcta acgcggagaa
      121 tgacgggctc cgtcggtgta gagccgtcga cgcgcacccg atgcgtcaac gttgtggtga
      181 gattttgcgc atccgtcgcc acaaggacaa attctccatt ggcgtcgacc gtgacttcaa
      241 aggtgaccaa accgtcgccc gccgaatcga tgatcggact gcccgctgcg gtaagcgatg
      301 tgagcggcga ttcatccgtc atcaatgcgg tgacgacgat cagcgcggtg ggcacaaccg
      361 cgtcgtagga gggcgataaa acgactatcg acggaggctc atcgtcgccg gaggcattca
      421 cgacggtgat agcgcgggtc tgctgcgcct gattaccggc gaggtcgagc acggctacga
      481 caatggactg cgctccgtca gcgagtgtga gacggcagcc aaactgccca tttggtagca
      541 acgcgactgt ttgtccgtta accgtgagcg aagaggcagg attggcatcc gtcaccgcgc
      601 ccacgacgtc gacgatcgat gtgccgatac tcgctgcggc ggccggccgg gacagcgtga
      661 tgaccggagc aatcgtgtcg gatatcaccg tgtgcaccac gggatcactt tcgttgccaa
      721 cgcggtcgac gacgcggatg aagatatccg tggctttgtt gggcgtaacg aggaccaaag
      781 cgctgaactc accggacgtg ttgacggtcg ctgaaacgga cacgagcccg ccgataatac
      841 tgacggttga ccccggctcc gcagtgccgg agagcgtaaa ccggtcagtc ttaatatacg
      901 tcggcggcgt tgcgatcgcc ggaggcggcg gagtccggtc cgaggtaacg gtgattgata
      961 cgggcgcaga tgcgttgccc aacccatcgg tggccaccgc ggtgagaaac gtttgtccct
     1021 cctcgagtga aaaatccgga atctcgaact ggccgtcggc aacagtggcc gtgcgcccgt
     1081 tgacgagaac ggccgccgtt gggtcgtcga ccgtgccggt gacgcgaatc accgcagcct
     1141 tagtggcgct accgttcgcc ggtgttataa acgcgatgtt cggcgcagtt gtgtcacgac
     1201 ggaccgtaat agacttgaag gcggtgttgc cggctgcgtc tttcgcctga acggtgaaag
     1261 tgttttcacc ggcggcgaga gaacgac
//
LOCUS       DASPLT010001000         2061 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_176365, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001000 DASPLT010000000
VERSION     DASPLT010001000.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 2061)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2061)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2061
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_176365"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1239
                     /locus_tag="VEA63_10355"
     CDS             <1..1239
                     /locus_tag="VEA63_10355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD84449.1"
                     /translation="RFGYWLAAMLVPLAVSGGVFWSRVTGLLERFMLFRAYDFGWRVP
                     VLTPEGWLGLVGDDQLNALPGSARLVLAVVFGTGLITALVVSARRRLRAVYASVCLLI
                     PALVGYAYLNLRGHFLGTHASYDAYKILAVFYPGLLPAAAFWAEWAWSRGRIVRAAVL
                     VVMAGVLVGNLRVAQRSAARLESPPFGVTRELIGLRELEAQPEIESVNLLMSDGWSRL
                     WANAQLLRIPQYFDVHTYEGRLNTPLRGQWDLIGGVIEIRLPGSGSRPAGPHFTLVDT
                     RNDFFFRVRFGAGWHDVELPFSSPSHWRWTTGEGELLVENPHTRPITTNLRLTARALE
                     TRNMEIWVNGQRSAIVPIGTDADTVGIPGVTLPPGETRLVFRSGGTVPPGDLRTLGVA
                     FYRIAFDVQPLRPLVESDWH"
     gene            1279..>2061
                     /locus_tag="VEA63_10360"
     CDS             1279..>2061
                     /locus_tag="VEA63_10360"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HYD84450.1"
                     /translation="MPDRLLTADPRAGFLAHEIEIRAAIERVLASGHYILGPEVEAFE
                     KEFAAYQEGGDVVGVANGTEAIELALRAVGVKAGDAVATVANTVSATAAAIEQMGARL
                     VFVEIDAATMLMSAEALEAVLERERGAVKAVVPVQLYGQVAEMERIGEIARRHGAKVV
                     EDCAQAHGAMREGRKAGTWGDAAAFSFYPTKNLGALGDGGAVFTRDAAVAQRVRQLRQ
                     YGWRERYVSELPGRNSRLDEVQAAVLRVKLRYLDAENATRREL"
BASE COUNT          317 a          498 c          834 g          412 t
ORIGIN      
        1 agatttggat actggctggc ggcgatgctg gttccgctcg cggtgagcgg cggggtgttt
       61 tggtcgcgtg tcacgggttt gcttgagcgg tttatgttgt ttcgcgcgta tgatttcggg
      121 tggcgggtgc cggtgctgac acccgaagga tggcttgggc tcgtcgggga tgaccagttg
      181 aacgcgttgc cagggagcgc gcggctggtg ctggcggtgg tgtttggaac gggcttgatc
      241 acagcgctcg tcgtaagcgc gcgacggcgg ctgcgggcgg tgtatgcgtc ggtctgcctg
      301 ctgattccgg cgctggtggg ttatgcgtat ttgaatctgc gcggacactt tctcggcacg
      361 catgcgagct atgatgccta caagattctg gccgtatttt atccggggct gctgcccgcc
      421 gccgcgtttt gggcggagtg ggcgtggtcg cgtggacgga tcgtgcgcgc ggcagtcttg
      481 gtcgtaatgg cgggtgtgct ggtgggaaac ctgcgcgtgg cgcaacgctc ggcggcgagg
      541 ctggaatctc cgccgtttgg tgtgacgcgc gaactgatcg ggctgcgcga actggaggcg
      601 caaccggaaa tcgaatccgt taacctgctg atgtccgacg gatggtcgcg gctgtgggcg
      661 aatgcgcagt tgttgcggat tccgcaatat ttcgacgttc acacctacga aggccggttg
      721 aacacgcctt tgcgcgggca atgggatctg atcggcggtg tgattgagat ccggctcccg
      781 ggcagcggga gtcgaccggc ggggccgcat tttactctgg tggatacacg caacgatttt
      841 ttctttcgcg tgcgattcgg cgccgggtgg cacgacgtcg agctgccgtt cagttcgccc
      901 tcgcactggc ggtggacgac gggggagggc gagctattgg tggagaaccc gcacacgcgc
      961 cccatcacga cgaacctgcg cctgaccgcg cgggcgctcg aaacgagaaa catggagata
     1021 tgggtgaacg ggcagcgcag tgcgatcgtg ccgatcggca cggatgcgga tacggtgggg
     1081 attccgggcg taacgctgcc accaggcgag acgcggctcg tgtttcgttc gggcggaacg
     1141 gtgccgccag gcgacttgcg gacgctcggg gtggcgtttt atcggatcgc atttgatgtg
     1201 cagccattga gaccgctcgt ggagtccgat tggcattgag ccggcgagcg aagacggcaa
     1261 acggatgtgt tcactctcat gcctgatcgt ctcctaacgg cggatccgcg cgccggattc
     1321 ctcgcgcatg aaattgaaat ccgcgcggcg atcgagcggg tgctggcgag cgggcattac
     1381 attctcgggc cggaggtcga ggcgtttgaa aaggagtttg ccgcgtatca ggaaggtgga
     1441 gacgtggtag gggtggcgaa tgggacggag gcgatcgagc tggcgttgcg tgcggtcggc
     1501 gtgaaggcgg gcgatgcggt ggcgacggtg gcgaacacgg tcagcgcgac ggcggcggcg
     1561 atcgagcaga tgggggcgcg gctggtgttc gtggagatcg atgcggcgac gatgctgatg
     1621 tcggcggagg cgttggaggc ggtgctggag cgtgagcgtg gcgcggtgaa ggcggtggtg
     1681 ccggtgcagc tttatggtca ggtggcggag atggagcgga tcggggagat cgcgcggcgg
     1741 cacggcgcga aggtggtcga ggattgcgcg caggcgcatg gcgcgatgcg cgagggaagg
     1801 aaggcgggga cgtggggtga tgcggcggcg tttagttttt atccgacgaa aaatttgggc
     1861 gcgctggggg atggcggcgc ggtgttcacg cgtgacgcgg cggtggcgca gcgcgtgcgt
     1921 cagttgcggc agtatgggtg gcgcgagcgt tacgtgagcg agctgccggg tcgcaacagc
     1981 cggctcgatg aagtgcaggc ggcggtgttg cgggtgaagt tgcggtatct cgatgcggag
     2041 aatgccacgc ggcgggaatt g
//
LOCUS       DASPLT010001001         3453 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_51489, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001001 DASPLT010000000
VERSION     DASPLT010001001.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 3453)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3453)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3453
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_51489"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..214
                     /locus_tag="VEA63_10365"
     CDS             <1..214
                     /locus_tag="VEA63_10365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009508974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD84451.1"
                     /translation="SVRDVSIAELQETLVGQGAEIGRTIGEPDWAAIEIAGQLPFDEP
                     PTTGDKDAASSHRTAWVADTVTAPER"
     gene            520..>3453
                     /locus_tag="VEA63_10370"
     CDS             520..>3453
                     /locus_tag="VEA63_10370"
                     /inference="COORDINATES: protein motif:HMM:NF028442.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HYD84452.1"
                     /translation="MIFRKKSLSLVLRCLVFAFLSATAKTHELATMFAQPPDEARARV
                     WWHWVDGNVSKEGITADLEAMQRVGIGGAWIFNVSQSLPEGPAPFLSSTWLRMLDHAV
                     EEADRLGLELGIHNSDGWSESGGPWITPEVSMKKLTWSETTVRGSGRKQLKLPQPEAL
                     MNFYREVAVLAVPAPRNDTAQKPSAIRTSFPLNEPQVLSDERENTAVSIPATAEADAQ
                     WIELDYEQPLVARALTLTTPELRYGVGKPPPSAQLEAWSGTGWEQIKEFNIRWSVDSS
                     PVKATTVAFDAVRAERFRITFFDLPAFELADIQLSEEPRVDYWQLKAGWIQVKEHGGE
                     TDYISARDVVRDNDGAAVAPKLTDVINLSSSMADDGALEWDVPEGEWVIYRIGLTTTG
                     RTNRPATPTGKGLECDKLSRKGIDAHFPHYAGRLAETYGPRAGRALRVVGTDSWEAGL
                     QNWTEDLPQEFRARRGYDIIPWLPLLLSGRAFESSAASERVLWDFRRTLADMIRENYF
                     EAFRELSHASGLTYWGESAGRTQYMYDPLNYQSAHDRPMGEFWVNQKLRPDNRAAASV
                     AHTYGRPIVTAEAYTAQPADGRWTNDPFRLKAQGDLAFCAGVNALTLHRYAHQPWMHV
                     APGLTMGRWGTMFDRTNTWWEMSRPWFDYLARAQYLLRQGEFVADVCHYIGEDVPNFI
                     GHREDLWLPLPAGVDYDGCNDEILRRFTVRNGRLVLPHGQSYAVLLLPESRTMRPDSL
                     RVIRDLIYAGATVIGRRPLRSPSFLDLGAGDAELQRLVDELWGPAPSDRTSLRHVGAG
                     RMLSVPAEGATWEEIARLLDFRPDFEAQPSTPANIHYIHRRVEDRDIYFVATDAAQSC
                     TAITRFRITGKRPQLWNPQTGEIRPLPEFTDDGAITTVPLQFDPSGSAFVVFAPNSES
                     DAPTEMKRNFPDYRTVLDVRGAWGVAFDPAWGGPAHIRFPTLIDWTQHDDKGIRYYSG
                     TATYRK"
BASE COUNT          684 a         1094 c         1030 g          645 t
ORIGIN      
        1 cagcgtgcgc gatgtgtcca tcgcggagtt gcaagaaacc ctcgtcggtc aaggcgccga
       61 gatcggccgc acgatcggcg agcccgactg ggcggcgatc gagatcgccg gccaactgcc
      121 cttcgatgag ccgccgacca cgggcgataa agacgcggcg tcctcgcatc gcaccgcctg
      181 ggttgctgat acggtgaccg cgcccgaacg ctgagtgagg ggcgcggcga ccgcgcaacg
      241 aacgtatccg ttcgatcgct acggcctaag ccggaaaaat tcagctcaac cacgaatgga
      301 cacgaatcga cacgattgaa atcgagttcg gacgcagaat cgattcggaa caccaaacgg
      361 tgatacacac gatcggccca aaagctttcc tctgcctcct ctgaattcgt gttcattcgt
      421 gtccattcgt ggtttctcaa ctgcatgaat ccggcaagcg cgcaggtttt tggccttggc
      481 cttcgcttcc cctcaatcgg tttgcccgcg tttcctttta tgatcttccg caagaagtcg
      541 ctctccctcg tccttcgctg cttggttttc gcttttctca gcgcgacggc aaaaacccac
      601 gaactcgcga cgatgtttgc gcagccgccg gacgaagcgc gtgcgcgcgt atggtggcat
      661 tgggtcgatg ggaacgtttc gaaagaggga atcaccgccg acctcgaagc gatgcaacgc
      721 gtgggcatcg gcggagcctg gatcttcaac gtgagccaat ccctgccgga aggtccggcg
      781 ccttttctct cttcaacctg gctgcggatg ctcgaccacg ccgtggaaga agcggaccgg
      841 ctggggctcg aattaggcat tcacaactcc gacggctggt cggagtcggg cggcccttgg
      901 atcacgccgg aggtttcgat gaagaaactc acgtggagcg aaacgaccgt gcgtggttcc
      961 ggccggaaac aactgaaact gccgcaaccg gaggcgctca tgaatttcta tcgcgaagtc
     1021 gccgtgctcg ctgttcccgc gccgcgcaat gacaccgcac agaaaccatc agccatccgc
     1081 acctcttttc cgttgaacga accgcaagtg ctgagcgacg agcgcgaaaa cacggccgtc
     1141 tcgattcccg ctaccgcaga agccgacgcg caatggatcg agctcgatta cgaacaaccg
     1201 ctcgtcgcgc gcgcgctcac gctgacgact cccgagctgc gttatggtgt gggcaaacca
     1261 ccaccgtccg cgcaactgga agcctggagt ggcacgggtt gggagcagat caaggagttc
     1321 aacattcgct ggtcggtcga ttcgagtccg gtgaaagcga ccacggtagc tttcgacgcc
     1381 gtgcgcgcgg agcgattccg catcacgttt ttcgacctac ccgcattcga actcgccgac
     1441 atccagttga gcgaagagcc gcgggtggat tactggcagc ttaaagccgg ctggattcag
     1501 gtgaaagagc acggcggcga aaccgattac atcagcgcga gagatgtggt ccgagataac
     1561 gatggcgctg ccgtcgcgcc gaaactcact gacgtgatca acctgagttc ctccatggcc
     1621 gatgacggcg cgctggaatg ggacgttccc gagggcgaat gggtcatcta tcgcatcggc
     1681 ctgacaacga ccggccgcac gaaccgcccc gcgaccccga ccggcaaagg tctcgaatgc
     1741 gacaagctca gccgcaaagg catcgacgcg catttccccc attacgccgg ccggctcgcc
     1801 gagacatatg gcccgcgcgc aggccgcgca ttgcgagtgg tgggaacgga tagctgggaa
     1861 gccggacttc aaaactggac cgaagatctg ccgcaggaat tccgtgcccg ccgcggatac
     1921 gacattatcc cgtggctgcc tttgctgctt tccgggcgcg ccttcgaaag ctccgcggcg
     1981 tcggaacgcg tgctctggga ttttcgccgc acgctggcgg atatgattcg cgagaactac
     2041 ttcgaggctt tccgcgagct cagccacgcg tccgggctga cgtattgggg agaaagcgcg
     2101 gggcgcaccc aatacatgta tgatccgctc aactaccaat cggcacacga ccgtccaatg
     2161 ggtgaattct gggttaatca aaaactccgg cccgacaatc gcgcggcggc ctccgtcgcg
     2221 cacacctatg gacgtccgat tgtcacggcc gaagcttaca ccgcgcagcc cgccgatggt
     2281 cgctggacga acgatccgtt tcgcctcaaa gcgcagggcg atctcgcctt ctgcgccggc
     2341 gtcaacgcgc tcacgctcca tcgctacgcg catcagccct ggatgcacgt ggcaccgggc
     2401 ctgacaatgg gccgctgggg aacgatgttc gaccgcacca acacctggtg ggagatgagc
     2461 cggccgtggt tcgattatct cgcgcgtgct caatacctgc ttcgccaagg cgaattcgtc
     2521 gccgacgtct gccattacat cggcgaagac gttcccaact tcatcggcca tcgcgaggac
     2581 ttgtggctgc cgctgccggc gggtgtcgac tacgacggtt gcaatgacga aatcctccgc
     2641 cgcttcaccg tgcgaaatgg acgactcgtg ctgcctcacg ggcagagcta cgctgttttg
     2701 ctgttgccgg agagccgcac aatgcgcccc gactcgctgc gggtgattcg tgatttgatt
     2761 tacgccggcg ccaccgtgat cggccggcgg ccgttgcgtt cgccaagttt cctcgacttg
     2821 ggtgccggcg acgccgagtt gcagcgactc gtggatgagt tgtggggacc ggcgccttca
     2881 gaccgcacat cgctacgaca tgtcggcgcg gggcgcatgc tcagcgtccc cgccgaaggc
     2941 gcaacctggg aggaaatcgc gcgactgctc gacttccgtc ctgatttcga agctcagccg
     3001 tccacgccgg cgaacattca ttacattcat cggcgcgtcg aggatcgcga catctacttt
     3061 gtggctacgg atgcggcgca atcgtgcacc gccatcactc gcttccgaat caccggcaag
     3121 cgtcctcaac tctggaatcc gcaaaccggt gaaattcgtc cgctgcccga gttcaccgac
     3181 gacggagcaa tcacgacggt gccgctccag ttcgatccgt caggaagtgc gttcgtcgtg
     3241 ttcgcgccga attcggagag cgacgcgccc accgaaatga agcgtaattt tccggattat
     3301 cgcacggtgc tcgacgtgcg aggcgcgtgg ggcgtcgcgt tcgatcccgc gtggggcggt
     3361 ccggcgcaca tccgctttcc aacattgatc gactggaccc aacacgacga caaaggcatc
     3421 cgctattaca gcggcaccgc gacgtatcga aaa
//
LOCUS       DASPLT010001002         3588 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_197203, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001002 DASPLT010000000
VERSION     DASPLT010001002.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 3588)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3588)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3588
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_197203"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1661)
                     /locus_tag="VEA63_10375"
     CDS             complement(<1..1661)
                     /locus_tag="VEA63_10375"
                     /inference="COORDINATES: protein motif:HMM:NF017263.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alginate lyase family protein"
                     /protein_id="HYD84453.1"
                     /translation="MHLVHPFSFGRPLRFLIGLVFAGALQSVTFAAFVHPGGLHTLAD
                     LDRMRDKVAAGESPWIDGWNRLIADTPTLLAYNPSPSANMGSSRQNCSRDAHAAYNAI
                     IRWYVSGDTAYADKAVQICNAWSAVVNQVPTGTDIPGLSGIPIFEFALVGELLRIYPG
                     WAPADFERFKTMMTTWWYPVCHDFLTNHNPPACTTHWWANWDASNVGALIAIGVLCDN
                     QAIYDEGVAYFQTGEGAGSMLNAVSFIHPNGLGQWQESGRDQEHAQLGVGLLATACEV
                     AWKQGLDLYGFMNNRLLAGAEYVARTNLSQPVPFVFYNNCDDVNHYFLSLNGLGRLDD
                     RPLWEMIYNHYVVRKGLSAPNVRKIAELMRPEHGSVDHFGYGTLTFTLDAAASPHPAA
                     PTPPVPAGVTALAGVGRVTLTWQPSPNDTAQGYRIQRSTTSGGPYTTIANWTDNTFPE
                     RTDTAVSNGTTYYYVVAANNVAGTSANSPEVSAIPMAAGALPDGWARQDIGAVNSAGG
                     ASYADVGNHTFIVAGNGTGIGGGVDSLSFAYGAVSGDCTITGRLLV"
     gene            complement(1819..>3588)
                     /locus_tag="VEA63_10380"
     CDS             complement(1819..>3588)
                     /locus_tag="VEA63_10380"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HYD84454.1"
                     /translation="IPDPRQWHAGNNQVIVRYYWDGSDRPSWGNLQPTSLFDRVPTTT
                     GGVTTYTTGTKPVTTRWVLSDWAFREEKTSSAIFAFAARYFDNKLIVSPGVRFDRHTT
                     YIRNRPGNWGTLPDDPTWDGLDVSDDRYWRPDAPSDWKSYIPTDRPVIGGVNDVNRPN
                     PLADDKRYRNDYNNPRAKSNLVSSTVGATYHLFPWVALKASYGESFLPGQVARFNLQG
                     EDVDPEMGKAFEGAVTFSLFNDRLAITPRYYHNLQENRLGFPVGAINDLMGRRAYNDN
                     NATGRNPFGFSNVPGGDFFKTKNDGIELEIAGNITRGWRIMANYGTAQATDLSGRFPA
                     AGEYVLSRVNDYRQVLEAAGGLLNTSAKPQNAGHAINDAPGLAVPNPAISDADIRAAG
                     GDPGVRQAAVNDYNNIWVQYDNYLLSRDVETLANKYKRINIFTDYTIQSGRLKGLTTG
                     IGWQFSDQVIAGYYSAQTIANPSFNASLPVSPSNLQYIDDPDVDVNSPVYAKRPSEVT
                     LTLGYERRLPKGWGMFGGKQISFQLTVRNLLDSRDTFYQDDGIVNRPPNGDPSLGYRE
                     TVVNRVALYQRPINFELQTTLSF"
BASE COUNT          684 a         1089 c         1144 g          671 t
ORIGIN      
        1 acgagcagcc ggccggtgat cgtgcagtcg ccgctcaccg caccatatgc gaagctcaac
       61 gaatcgactc ctccgccgat gccggtgccg ttgcccgcga cgatgaacgt gtggttgccg
      121 acgtcggcat aactcgcacc accggcagag ttcaccgcgc cgatatcctg ccgcgcccaa
      181 ccgtccggca gcgcaccggc ggccatcggt atcgcgctca cctcagggga attcgcactc
      241 gttccggcaa cattgttcgc tgcgacgacg tagtaatagg ttgtcccatt gctgaccgcg
      301 gtgtcggtgc gctcgggaaa cgtgttgtcg gtccagttcg caatcgtcgt gtaaggtccg
      361 ccgctcgtcg tggaacgctg gatacgataa ccctgagcgg tgtcattcgg cgacggctgc
      421 caggtcagcg tcacacgccc gacaccggcg agcgcggtga cgcccgccgg caccggcggc
      481 gtcggcgcgg ccggatgcgg tgaggcggcg gcatcgagcg tgaacgtcaa cgtgccgtag
      541 ccgaagtgat ccacgctgcc gtgctcgggg cgcatcagct cagcgatttt gcgaacgttc
      601 ggcgcactca ggcctttgcg cacgacgtag tgattgtaga tcatctccca gagcggacgg
      661 tcgtcgagcc ggccgagtcc gttgagcgac agaaaatagt gattcacgtc gtcgcagttg
      721 ttgtagaaca cgaacggaac cggctgggaa agattcgtgc gcgcgacgta ttcggcgccg
      781 gcgagcagcc ggttgttcat gaagccgtaa aggtcgagac cctgcttcca cgcgacttcg
      841 cacgcggtgg cgagcaggcc aacaccaagt tgcgcgtgct cctgatcgcg cccgctttcc
      901 tgccactggc cgagtccatt cggatgaatg aacgacaccg cgttcaacat gctgcccgcg
      961 ccctcgcccg tttggaaata cgccacaccc tcgtcgtaga tcgcctgatt gtcgcacaac
     1021 acgccgatgg cgatcagcgc tccgacgtta ctcgcatccc agttcgccca ccaatgcgtc
     1081 gtgcacgccg gaggattatg attcgtgaga aagtcatgac aaactggata ccaccaggtc
     1141 gtcatcatgg tcttgaaccg ctcgaagtcc gccggcgccc aacccggata aatgcgcaac
     1201 aactcgccga cgagcgcgaa ctcgaagatc ggaatcccac tcaggcccgg gatgtcggtg
     1261 ccggtcggca cctgattcac gacggccgac cacgcgttgc agatctgcac cgccttgtcc
     1321 gcgtaagccg tatcgcccga gacataccag cggatgatcg cgttgtaagc agcgtgcgcg
     1381 tcgcgcgagc agttctgccg gctgctcccc atgttggccg atggactcgg attatacgcg
     1441 agcagcgtcg gcgtatcggc gatgagccgg ttccaaccgt cgatccacgg actctcgccc
     1501 gcagcaactt tgtccctcat gcggtcgaga tcggcgagcg tgtgcagacc gccgggatga
     1561 acgaacgcgg cgaacgtcac ggactgcagc gcgccggcga acaccaaacc gatcagaaaa
     1621 cgcagcggcc gaccaaacga gaaggggtga actaggtgca tggcttgggg aatggggaaa
     1681 cgggaaactt agcgggaaac gcgggcgcct ggttgcgccc cgcggaggaa aagttgcgcg
     1741 tcacgagcca cgctcctgga aagcttcacc cgcgcgaaac gatggcggcg caggtgaacc
     1801 gagcgcggac gtttgcgcct agaacgagag cgtggtctgc agctcgaagt tgatcggccg
     1861 ttgatacagc gcgacgcggt tcacgaccgt ttcgcgatag ccgagcgacg gatcgccgtt
     1921 cggcgggcgg ttcacgatgc cgtcgtcctg ataaaacgtg tcgcgcgaat cgagcaggtt
     1981 gcggaccgtg agttgaaaac tgatctgctt gccaccgaac atcccccagc ctttcggcag
     2041 gcgacgctcg taacccagcg tgagcgtcac ctcggagggg cgcttggcgt aaacggggct
     2101 gttcacatcc acgtcggggt cgtcgatgta ttgcagattc gacggcgaga ccggcagcga
     2161 cgcattgaag ctcggattcg cgatcgtctg cgcggaataa tagccggcga tgacttgatc
     2221 ggagaactgc cagccaatgc ccgtggtcaa acccttcaga cgcccggact gaatcgtgta
     2281 atcggtgaat atattgattc gtttatattt gttggcgagc gtctcgacgt cgcggctgag
     2341 caggtaatta tcatactgca cccagatgtt gttgtaatcg ttgaccgcgg cctgacgcac
     2401 gccggggtcg ccgcccgcgg cgcggatatc ggcgtccgag atcgcgggat ttgggaccgc
     2461 gaggccgggc gcatcgttga tcgcgtgacc ggcattctgc ggcttcgcgc tggtgttgag
     2521 caggccaccg gccgcttcca acacctgcct gtaatcgttc acgcggctga gcacgtattc
     2581 gcccgcagcc ggaaaacgtc cgctgagatc ggtcgcttgg gcggtgccgt agtttgccat
     2641 gatgcgccag ccacgcgtga tgttgccggc gatctcgagc tcgatgccgt cgttcttcgt
     2701 tttgaaaaaa tcgccgccgg ggacgttgga gaacccgaac ggatttcggc ccgttgcgtt
     2761 gttgtcgttg taagcgcgac ggcccatgag atcgttgatc gcgccgacag ggaagccgag
     2821 gcggttttcc tgcaggttgt gataataccg cggcgtgatc gccagccggt cgttgaagag
     2881 actgaaggta accgcgccct cgaaggcctt gcccatctcg ggatcgacgt cctcgccctg
     2941 gaggttgaaa cgcgccacct ggccgggcag gaagctctcg ccgtagcttg ccttgagcgc
     3001 gacccaggga aagaggtgat acgtggcgcc gacggtcgag ctcacgagat tcgatttcgc
     3061 gcgcggatta ttatagtcgt tgcgataacg tttgtcgtcc gcgagcgggt tgggccggtt
     3121 cacatcgttt acgccgccga tcacaggccg gtcggtcgga atgtaggact tccaatcgga
     3181 aggcgcgtcc ggacgccagt agcggtcgtc gctcacatcg aggccgtccc acgtcggatc
     3241 gtccgggagc gtgccccagt ttcccgggcg gttgcgaatg taagtggtgt ggcgatcgaa
     3301 gcggacaccc ggcgaaacga tgagcttgtt gtcaaagtaa cgcgcggcga acgcgaagat
     3361 ggccgacgac gttttctcct cgcgaaacgc ccagtccgac agcacccaac gcgtcgttac
     3421 cggcttcgtg cccgtcgtgt aagtggtcac gccaccggtc gtcgtcggca cgcgatcgaa
     3481 gaggctggtc ggctggagat ttccccagga cggacgatct gaaccgtccc aatagtaacg
     3541 cacgatgacc tgattgtttc cggcgtgcca ctggcgcgga tcaggaat
//
LOCUS       DASPLT010001003         1128 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_12650, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001003 DASPLT010000000
VERSION     DASPLT010001003.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1128)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1128)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1128
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_12650"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1091)
                     /locus_tag="VEA63_10385"
     CDS             complement(<1..1091)
                     /locus_tag="VEA63_10385"
                     /inference="COORDINATES: protein motif:HMM:NF025930.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HYD84455.1"
                     /translation="MGKKDVEAMAKERTKFVEGAKRVNNIDEKIANSIFDILNKFAGY
                     GFNKSHSAAYALLSYQTGYLKANYPVQFMAAMLSSELGNSEKVSHFVAECEAMGLTVL
                     GPDVNESRENFTPVFRKKAESPESRAESQNSELSALSSQLSAENRGSAAIRFGLAGVK
                     GVGELAAQKIIEERDKNGPFKDFDDFISRVDGRAINKRVLEHLSKTGAFDFSGASRKK
                     LFENIDAAMAAVAASARDKAAGQHSFLDMFNEPPARNLKSEISNLKSAAEGGDDFTSA
                     ERLAFEKELLGFYVSGHPMNDYAGLTDAIDTFPIDQLLSQPDRTEFRLCGIVSNIAKR
                     LSKKDNRPWASFTLATKTASVPLNMFADS"
BASE COUNT          192 a          273 c          400 g          263 t
ORIGIN      
        1 taggagtcgg cgaacatgtt gagcgggacg gaggcggttt tcgtggcgag cgtgaagctg
       61 gcccacgggc gattgtcttt tttcgagaga cgtttcgcga tgttgctgac gatgccgcag
      121 aggcggaact cggtgcggtc gggctgcgac aggagctggt cgatggggaa cgtgtcgatc
      181 gcgtcggtga gtccggcgta atcgttcatc gggtggccgg agacgtagaa gccgaggagt
      241 tctttttcga aggcgaggcg ttcggcggag gtgaagtcgt cgccgccttc ggcggcggat
      301 ttcaaatttg aaatttcaga tttgagattc cgagccggag gctcgttgaa catatccagg
      361 aagctgtgct ggccggcggc tttgtcgcgg gcgctcgcgg cgacggcggc catcgcggcg
      421 tcgatgttct cgaaaagttt tttgcgcgag gcgccgctga aatcgaaggc gccggttttg
      481 ctgaggtgtt cgaggacgcg cttgttgatc gcgcggccgt cgacgcggga aatgaagtcg
      541 tcgaaatcct taaacggacc gttcttgtcg cgttcctcga tgattttttg cgcggcgagt
      601 tcgccgacgc ccttcacgcc ggcgaggccg aagcgaatcg cggcggagcc gcgattctca
      661 gctgagagct gagagctgag agctgagagt tcggaattct ggctctcagc tctggactct
      721 gggctctcag ctttcttgcg gaaaacgggc gtgaaatttt cgcgcgactc gttcacgtcg
      781 gggccgagga cggtgaggcc catggcttcg cattcggcga cgaagtggga gactttttcg
      841 gagttgccca gttcggagct gagcatggcg gccatgaact gaaccgggta gttggctttc
      901 aaatacccgg tttggtagct gagcagcgcg taggcggcgg agtgggattt gttgaaaccg
      961 tagccggcga atttgttgag gatgtcgaag atcgagttgg cgatcttctc atcgatgttg
     1021 ttcacgcgtt tggcgccctc gacgaatttg gtgcgctcct tggccatggc ctcgacgtct
     1081 tttttgccca tggcgcggcg aagcatgtcg gcgccgccga gggtataa
//
LOCUS       DASPLT010001004         1066 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_35579, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001004 DASPLT010000000
VERSION     DASPLT010001004.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1066)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1066)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1066
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_35579"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..655
                     /locus_tag="VEA63_10390"
     CDS             <1..655
                     /locus_tag="VEA63_10390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD84456.1"
                     /translation="VGTWLDSLDWSRAWRTSHLFWGGVHCFSFSARANAAWLEATFAW
                     LEANLDPATGWWREGVEHADRHQALGGAAHLFPLFEHHGRPFPYPERVIDSVLALQLP
                     DGRWLHPRNRTDRLHVMHYLELDALYVLALMSNYSPEYRRDDVLYAARRFGDLVRRYY
                     ETRAEELYALHPHRVLAAIGAFGGLQQLLPEEFVGDVEWTDIFSDRRFYRTRAVERL"
     gene            652..>1066
                     /locus_tag="VEA63_10395"
     CDS             652..>1066
                     /locus_tag="VEA63_10395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009509460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD84457.1"
                     /translation="MIFNSSNHETPNGVRPILSLNGLWDFAYLGPIDLNLFSPKMVKF
                     EEKTLVPSAFDALPAHAGKRGAAVYRTRFLVPSGRPARIEFGAVSMWTRVFVDGKLER
                     EHACGYAPFVVEVAPASHVERELLVLVDNRFDFERV"
BASE COUNT          169 a          314 c          357 g          226 t
ORIGIN      
        1 ggttgggaca tggctcgact cgctcgactg gagcagggcg tggcgaacgt cgcatctttt
       61 ctggggcggc gtgcactgct ttagttttag cgcgcgggcg aacgccgcgt ggttggaggc
      121 gacgtttgcc tggctcgagg cgaacctcga cccggcgacg ggctggtggc gtgaaggcgt
      181 cgaacacgcc gaccgtcatc aggcactggg tggcgccgcg catttgtttc cgctcttcga
      241 gcaccacggg cggccatttc cttatcccga gcgagtgatc gacagcgtgc tggcgctgca
      301 gcttcccgat gggcgctggc tgcatccgcg gaaccgaacg gaccggcttc acgtcatgca
      361 ctatctggag ctcgacgcgc tctacgtgct cgcgttgatg agcaattaca gtcccgagta
      421 tcgtcgcgat gatgtccttt atgcggcgcg gcgattcggc gacttggtgc gccgctatta
      481 cgaaacgcgt gccgaggaac tttacgctct gcacccgcac cgggtgctgg ccgcgattgg
      541 cgcatttggc ggactgcaac aactgctgcc ggaagagttc gtcggtgatg tcgagtggac
      601 tgacatcttc agcgatcggc gcttttatcg cacgcgagcc gtggaacgat tatgatattc
      661 aactcatcca atcacgaaac gccgaacggg gtgcggccca ttttatcact gaacggattg
      721 tgggacttcg cctatctggg gccgatcgac ctcaacttgt tttcgccgaa gatggtgaag
      781 tttgaggaaa agacgctggt gccctcggca ttcgacgcct tgcccgcgca tgccgggaag
      841 cgcggcgccg cggtttatcg gacgcgtttt ctcgtgccgt cgggccggcc ggctcgcatc
      901 gagttcggcg cggtgtcgat gtggacgcgc gtgttcgtcg acgggaaact ggagcgcgag
      961 cacgcctgtg gctacgcgcc gttcgtcgtc gaggtggcgc cggccagcca cgtcgagcgc
     1021 gagcttctcg tgctggtcga caaccgcttc gacttcgagc gcgtgc
//
LOCUS       DASPLT010001005         1314 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_72162, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001005 DASPLT010000000
VERSION     DASPLT010001005.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1314)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1314)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1314
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_72162"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..718)
                     /locus_tag="VEA63_10400"
     CDS             complement(<1..718)
                     /locus_tag="VEA63_10400"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HYD84458.1"
                     /translation="MNVDSLSRRDFVKGIAVAGIALQGRPIFAQGSPAKRRFKVGLIG
                     CGGRGRGALANLLEAAPLAGAEIEIYALADVFPDRIAEVSGKFAVPAERCFTGFDAYR
                     KVLEQPIDIACLVTPPNFRPVHFEAAVAAGKHCFVEKPVAVDPPGCRRMLAAGEIARE
                     KGLSVLAGAQRRHLSAYLQNEHAIREGAIGRIIGGNVMWNQDRLWFKERLPGESNAHY
                     MARNWVNFLETSGDHIVEQHF"
     gene            complement(883..>1314)
                     /locus_tag="VEA63_10405"
     CDS             complement(883..>1314)
                     /locus_tag="VEA63_10405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HYD84459.1"
                     /translation="LLRAKPPNLGVWSPIGGKLETAIGESPFECAVRETMEETGHAIT
                     TADLHLFAMIAEKAYEGESHWLMFLFRCKRPLSVVPAEIHEGRFAFFSRAAIDALPIP
                     ETDRTALWPIYDRYRDRFVSLRADCAPGKPVVVEIEEITPS"
BASE COUNT          248 a          369 c          451 g          246 t
ORIGIN      
        1 ggaaatgttg ttcgacgatg tgatcgcccg acgtttcgag gaagttgacc caattgcgcg
       61 ccatgtagtg cgcattgctt tcgccgggga ggcgttcctt gaaccagagg cggtcctgat
      121 tccacatgac gttgccgccg atgatccggc cgatggcgcc ttcgcggatc gcgtgttcgt
      181 tttgcagata cgcgctcaag tgacgccgct gggcgccggc gaggacggaa aggcctttct
      241 cgcgcgctat ttcaccggcg gcgagcatgc gacgacagcc gggcggatcg acggcgaccg
      301 gtttctcaac gaaacagtgt ttgccggcgg cgaccgcggc ctcgaaatgc accggacgaa
      361 aatttggcgg cgtgacgagg caggcgatgt cgatgggttg ttcgaggacc ttgcgataag
      421 cgtcgaaacc ggtgaagcag cgttcggccg gcacggcgaa tttgccggaa acttcggcga
      481 tgcgatcggg gaaaacgtcg gccagcgcat agatctcgat ctcggcgccg gcgagtggcg
      541 cggcttcgag gaggttggcg agcgcgcccc ggccccgacc gccgcagccg atcaagccga
      601 ctttgaagcg gcgtttggcg ggcgagcctt gcgcgaaaat cgggcggcct tgcagggcaa
      661 ttccagcgac ggcgatgcct ttgacgaagt cgcgtcgcga cagtgaatcc acattcatac
      721 gtgctcctct ttttggggtg taaccagaac cgtcgcgagg ctcagcggaa gcaacgctcc
      781 ggtcgaggcg gaactcggtt tcgagaagtt attctccgcg ctgtttgcgg agatcggaaa
      841 cgatgatcac tgcgcgaggc accggcttcc ggccctgcgc acttatgacg gagtgatttc
      901 ctcgatctcg acgacgacag gtttgccggg cgcacagtcg gcgcggagtg aaacgaagcg
      961 gtcgcgatag cggtcgtaaa tcggccagag tgcagtgcga tcggtttcgg ggatcggcaa
     1021 ggcatcgatc gcggcgcgac tgaagaacgc gaagcgacct tcgtgaatct cggccggcac
     1081 gacggacaac ggccgtttgc agcggaagag aaacatcagc cagtgcgatt cgccctcgta
     1141 ggccttttcg gcgatcatcg cgaagaggtg gaggtcggcg gtggtgatgg cgtggccggt
     1201 ttcttccatc gtttcacgga cagcgcactc gaacggcgac tcgccgatgg cggtttcgag
     1261 cttgccgccg atgggactcc agacgccgag attcggcggt ttcgcacgaa gcaa
//
LOCUS       DASPLT010001006         2600 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_51525, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001006 DASPLT010000000
VERSION     DASPLT010001006.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 2600)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2600)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2600
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_51525"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            70..>2600
                     /locus_tag="VEA63_10410"
     CDS             70..>2600
                     /locus_tag="VEA63_10410"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HYD84460.1"
                     /translation="MSAILILQSAGLVSGQQAPAPVATEDEEPVVLSPFTVDASQDRG
                     YRATSTLAGSRINTNLRDVAASITEVTPEFLSDVAAVDINDVLAYMANTESTFNYTSA
                     PSQGIGGFADNVATNPQTANRVRGMSSATLTRDYFITIGDDIGFDSYNVDRITINRGP
                     NSILFGLGDPSGVVNFTPKVAKPNRNSNELSFRYGSHDDMRATADFNRVLIPDKLALR
                     VAGLWSDRGFKQQPSYYRDDRVNVMATYKPFEKTTLQASFEKTKLRANSPNAITPLDQ
                     ITKWIEQGRPTWDPSTQQWDDRPENFASLTGGGFVGPTRPDGSLEYTYQEGAGQATRA
                     TFWAPNTPGVLVYNSIGVSDSRYVPLHTMNLLPSVRNADMDVFTASWDQQITTDLYFN
                     VAYLNEQLDNESFSWTRTNQYGVFVDVNTHLPDGRQNPHFGETFMPQRSLDSRGVTEN
                     QNEAVRGTLNYNLDLTRQTNWTRWIGRHVFTGYAERRESDFVFDGYNGTRTGTPSYLN
                     AADRINNEGWQITRLRYLGGTAEKAATAAPGVPKTGESGIPNRYYNGTTGMWESDTFG
                     EFFALKRRDLGTNLVKSRAGVWQGYLLSDRIVGTVGLRHDENTVASKASTSIDPATGL
                     LRVDDTLGDPNTVSGNTKTYGVVAHPFKWLSLHYNRSENFVALAGDVNVYGESIAPPS
                     GKGKDYGFSLDLMEGDLNVRVNWYEVAQTNSRTTNSGPDILAQWELAWFDQVVIPRLA
                     EQYGLTHDTFFSPIGWGDNRIEETADVVSKGVEVEVVYNPTANWRIMANVSKQKASRS
                     NIAPGLTRWIEETLPTWQAAPWFNGPETYDAGWGVDGNLATYFSS"
BASE COUNT          523 a          830 c          793 g          454 t
ORIGIN      
        1 cccctgttcc ccatgcacct agccaccgta acgaacaccc cagccccaca ccgccgaggc
       61 gtgcgcctct tgtccgcgat tttgatcctg cagagcgccg gcttggtctc gggccagcaa
      121 gcgccggcac ccgttgcgac cgaggatgag gaaccggtcg tgctctcgcc tttcacggtc
      181 gacgcgtcac aggatcgtgg ataccgagcg acgagcaccc tcgccggcag tcgcatcaac
      241 accaatctcc gggatgtcgc cgcctcgatc accgaggtga cgcccgagtt cctgagcgac
      301 gtggccgccg tcgacatcaa cgacgtgctc gcttacatgg cgaacaccga gagcacgttc
      361 aattacacga gcgcgccgtc gcagggcatc ggcggcttcg ccgacaacgt ggcgacgaat
      421 ccgcagacgg cgaatcgcgt gcgcggcatg agcagtgcga cgctgacgcg cgattatttc
      481 atcacgattg gcgacgacat cggcttcgat tcctacaacg tcgatcgcat cacgatcaac
      541 cgcggcccga actccattct cttcggtctc ggcgacccgt cgggcgtggt caatttcacg
      601 ccgaaagtcg cgaagccgaa ccgcaactcg aacgaactca gtttccgcta cggttcgcat
      661 gacgacatgc gcgcgacggc ggatttcaat cgcgtgctca ttcccgacaa gctcgcgctt
      721 cgtgtcgcgg gcctctggtc ggatcgcggt ttcaagcagc agccctccta ttatcgcgac
      781 gatcgcgtca acgtgatggc cacctacaag cccttcgaga aaacgacgct tcaggccagc
      841 ttcgagaaaa ccaagcttcg cgcgaacagc ccgaacgcca tcacgccgct cgatcaaatc
      901 accaagtgga tcgagcaagg gcggccgacc tgggatccga gcacgcagca gtgggatgat
      961 cgcccggaga atttcgcttc gttgaccggc ggcggtttcg tcggcccgac gaggcccgac
     1021 ggttcgctcg agtatacgta tcaggaaggc gccggccagg cgacacgcgc gacgttctgg
     1081 gcgccgaaca cgccgggcgt gctcgtttat aacagcattg gcgtctccga cagccgctac
     1141 gtgccgctgc acacgatgaa cctgctgccg tcggtgcgaa acgccgacat ggacgtgttc
     1201 acggcgagtt gggaccagca gattaccacc gatctttatt tcaacgtcgc ctacctcaac
     1261 gagcagctcg acaacgagtc gttcagttgg acgcgcacga atcaatacgg cgtgttcgtc
     1321 gacgtgaaca cgcacctgcc ggacggtcgc cagaatccgc acttcggcga gacgttcatg
     1381 ccgcagcgct cgctcgacag ccgtggtgtc acggagaacc agaacgaagc ggtccgcggc
     1441 acgctcaact acaacctcga tctgacgcgc cagacgaatt ggacgcgctg gatcggccgg
     1501 cacgtgttca ccggttacgc cgagcggcgc gagagcgatt tcgtattcga cggctacaat
     1561 ggcacgcgca ccggcacgcc gtcttatctg aacgcggcgg accgcatcaa caacgagggc
     1621 tggcagatca cgcggctgcg ctatctcggc ggcaccgccg agaaagcggc gacggcggcc
     1681 ccgggcgttc ctaagacggg cgaaagcgga attccgaacc gctattacaa tggcacgacc
     1741 ggtatgtggg aatcggatac gttcggcgaa ttcttcgcgc tcaagcgccg cgatctcggc
     1801 accaatctcg tcaagtcgcg agccggtgtc tggcagggtt acctgctgag cgatcggatc
     1861 gtcggcacgg ttggtttgcg gcacgacgag aacacggtgg ccagcaaggc gtcgacctcg
     1921 atcgatccgg cgacgggcct gctcagggtc gacgacacgc tcggtgaccc gaataccgtc
     1981 agcggcaaca cgaagaccta cggcgtcgtc gcgcatccct tcaagtggct ctcgctgcac
     2041 tacaatcggt ccgagaactt cgtggcgctc gcgggcgacg tgaatgttta cggcgagtcg
     2101 atcgcgcccc cgtccggcaa gggcaaggat tatggtttct cgttggacct gatggagggc
     2161 gatctcaacg ttcgggtgaa ctggtatgag gtggcgcaaa cgaattcgcg cacgacgaat
     2221 tccggtcctg acattctcgc gcagtgggag ctcgcgtggt ttgatcaagt ggtgattccg
     2281 cggctcgcgg agcaatacgg gctcacgcac gacacgtttt tctcgccgat cggctggggc
     2341 gataatcgca tcgaggaaac cgccgacgtc gtttcgaaag gcgtcgaagt cgaagtcgtt
     2401 tacaacccga ccgcgaactg gcgcatcatg gcgaacgtga gcaagcagaa ggcctcgcgc
     2461 tcgaacatcg cgcccggtct cacgcgctgg atcgaggaaa cgctgccgac gtggcaggcg
     2521 gcgccgtggt tcaacggacc cgagacctac gacgcgggtt ggggcgtcga cggaaatctc
     2581 gcgacttatt tcagcagctt
//
LOCUS       DASPLT010001007         1261 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_277640, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001007 DASPLT010000000
VERSION     DASPLT010001007.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1261)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1261)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1261
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_277640"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(148..1209)
                     /locus_tag="VEA63_10415"
     CDS             complement(148..1209)
                     /locus_tag="VEA63_10415"
                     /inference="COORDINATES: protein motif:HMM:NF012576.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HYD84461.1"
                     /translation="MAAPSVSSNEPRPTVRMIAARAGISGPAVSMALRDHPRISEATR
                     KRVLRIAEEMGYRPDPEIAKLMNHLRQRRKPKFRSVITALTSIPEKEERPYNRQMIKG
                     AEARADQLGYRLSILRITPSVTRDHSLQRILRARGVDGLLLLPMRDTVPLTDLLDWSQ
                     FSVVAATRGVAEPVFHRVLPHHFGNTLEICDQLERLGYKRIGLVSNRGFDLFTSPGLA
                     AGVVWQHIQGRAEQVTPLLHDGERPAGVKAWFKRERPDAIVARGVPDAEAIVDDLGLK
                     FPGRIGLAVTNLEGSRSFAGMDVHPYDIGATAIEMVHARIQSSQKNVPRLLTETMITG
                     TWIAGPTVRAAADRKTRGR"
BASE COUNT          211 a          419 c          396 g          235 t
ORIGIN      
        1 gcctgcctgc atgtttttcg agaagggtag gtggtgcgct tgcgcaccac gttgcgtgcg
       61 tgcgcgcaac ccacaagagc agacaggcac ggagtcctgc cgctaccgct gtcgggctct
      121 acgttaccaa gccaccgcct cgcgtgctca acggcctcgc gtcttgcggt ccgccgcggc
      181 gcgcaccgtt ggcccggcga tccacgtccc cgtgatcatg gtttcggtca gcagtcgcgg
      241 cacgtttttt tggctgctct gaatacgtgc gtggaccatc tcgatcgccg tcgcaccgat
      301 gtcgtaaggg tgaacatcca tgcccgcgaa acttctcgac ccttcgagat tcgtcaccgc
      361 caggccgatc cgtccgggaa atttcaaacc gagatcgtcg acgatcgctt cggcatccgg
      421 cacgccgcgg gccacgatcg cgtcgggccg ctcgcgcttg aaccacgcct tcacgcccgc
      481 cgggcgttcg ccgtcgtgca gcagtggcgt cacctgttcc gcgcgccctt gaatatgttg
      541 ccacacgacg ccggccgcca ggccgggcga cgtgaaaaga tcgaagcctc ggttgctcac
      601 gaggccgatc cgcttgtagc cgagccgctc gagctgatcg cagatttcca gcgtgtttcc
      661 aaaatgatgc ggtagcacgc gatgaaacac cggctccgcc acgccgcgcg tcgcggccac
      721 aacggagaac tggctccagt cgagcagatc ggtgagcggc actgtgtcgc gcattggcag
      781 cagcaaaagt ccgtccaccc cacgcgcgcg gagaattcgt tgcaggctgt gatcgcgcgt
      841 caccgatggc gtgattcgca ggatcgagag ccggtagccg agctgatccg cgcgcgcctc
      901 cgctcccttg atcatctggc ggttgtaagg acgttcctcc ttttccggga tgctcgtcag
      961 cgcggtgatc accgagcgaa atttcggttt ccgccgctga cgcagatgat tcatgagctt
     1021 cgcgatctcg ggatcgggcc ggtaacccat ctcctccgcg atgcgcagaa cgcgtttgcg
     1081 cgtggcctcg gaaatgcgcg ggtgatcgcg caatgccatg gaaaccgcgg ggccgctgat
     1141 gccggcgcgc gccgcgatca tgcgcacggt cggacgcggt tcgttcgaag aaacggaggg
     1201 ggccgccatt cgcctcattg ccgcgctcaa cggttaagca gtcaagcgcg cgacgcgcgg
     1261 c
//
LOCUS       DASPLT010001008         1275 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_257735, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001008 DASPLT010000000
VERSION     DASPLT010001008.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1275)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1275)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1275
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_257735"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..839)
                     /locus_tag="VEA63_10420"
     CDS             complement(<1..839)
                     /locus_tag="VEA63_10420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HYD84462.1"
                     /translation="MHRRFLSSVAPSVRARLWFLVAVVAVPAALLSGWLIFRSYRTEW
                     LAMERRLVENARGFSALIDAELRERVAMAQSLAGTHALLSGDLAAFRVRAERLVVRPG
                     EWVMLVDADGRELVNTRLPADAELSRVAIAAEIAAAPDRALPYVSNLIVADETPRHSV
                     WVMARARIGNDDSGFVILALTPEALQASLMRDRLRDRRVIAVIDRNQIVVARSRAPEA
                     FVGEQATAQIRQAAAGANEGVTESVTLDGYPSIAAFSTSTESGWMVVVASHKSDLFDP
                     AKQ"
BASE COUNT          257 a          458 c          365 g          195 t
ORIGIN      
        1 atctgcttcg ccgggtcgaa cagatcggac ttgtggctgg caacgaccac catccaaccg
       61 ctttcagtcg acgtgctgaa cgcggcgata ctcggatagc catccaacgt caccgattcg
      121 gtcacgcctt cgttcgcccc cgccgccgct tgccggatct gcgccgtcgc ttgttcgccg
      181 acgaaagcct cgggcgcgcg gctccgcgcg accacgatct ggtttcgatc gatcaccgcg
      241 atcacgcgcc gatccctcaa ccggtcgcgc atcagcgagg cctgcaaagc ctccggcgta
      301 agcgcgagga tcacaaaacc cgagtcatcg tttccaatcc gcgcccgcgc catcacccac
      361 accgaatgtc gcggcgtttc gtccgccacg atcaggttgg aaacatacgg caaagcgcgg
      421 tcgggggccg cggcgatctc ggcggcgatg gcaacgcgtg acaactccgc gtccgccggc
      481 agccgggtgt taacgagctc gcgcccgtcc gcgtccacca gcatcaccca ctcgcccggc
      541 cgcacgacga gcctctcggc gcgcacgcga aatgccgcca gatctccgct caggagcgca
      601 tgcgtccccg caaggctctg cgccatagcc acgcgctcgc gcaattccgc atcgatcaac
      661 gcggaaaacc cgcgggcgtt ttcgacgaga cgccgctcca tcgcgagcca ctcggtgcga
      721 tagctacgaa agatcagcca gcccgacagc agcgccgccg gcaccgccac aacggcgacc
      781 agaaaccaca gtcgcgcccg gaccgaagga gcgacggacg aaagaaaacg acgatgcatc
      841 agacagctcg gatgcagacg acaagagcct cgggccgagg tcagctccag ccgaaagcga
      901 aattcggcta atgccgttaa ccggcattga gcgtttcggc gccgccgctc agcgtgagcc
      961 gcgccgcagc ctctcacacg cagccatcct acaacgccgc ttcaaagccg cgagcaggtc
     1021 cctgccctcc agcgagtagg gtcgcgcgtt cgaccgcgaa ggcttcaccg cgagaattgt
     1081 ttttgtgcgg ttcacacctt cgccacgagc atcgctcagg gaaaaacgtg cgccgcctcg
     1141 aggctttccg ctacttcctt ccggcatttc tccggggtga aatccaactc caatgatccg
     1201 accgcggcgc aatcaaaggc ggaggaggaa acgagccccc gctcgtcgtc gctaggaaag
     1261 tagtgcggtg cttta
//
LOCUS       DASPLT010001009         1247 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_135392, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001009 DASPLT010000000
VERSION     DASPLT010001009.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1247)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1247)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1247
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_135392"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..896)
                     /gene="asd"
                     /locus_tag="VEA63_10425"
     CDS             complement(<1..896)
                     /gene="asd"
                     /locus_tag="VEA63_10425"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374796.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="archaetidylserine decarboxylase"
                     /protein_id="HYD84463.1"
                     /translation="MPTEPIRFYHRYKKAVKTEKIFGEEWLRFAYDNPAGRFFVWLLA
                     RRKIFSWWYGRKMNKRVSALQILPFITKYDIDVDEFAKSAFDYKTFNEFFYRALKPEA
                     RPIAAGERVAVFPADGRHLAFADVETTGGFYVKGAQFTLAELFGEGKDADGPPQLAAK
                     FAGGAMVISRLCPVDYHRFHFPVAGVPGEARLINGYLYSVSPVALRRNLHYLVQNKRM
                     LTLIESAAFGTVAMFEVGATNVGSIRQTYLPNRAVEKGAEKGLFAFGGSCVITVFQRG
                     RIRFDDDVLGPSREQMEVYVRMG"
BASE COUNT          255 a          448 c          332 g          212 t
ORIGIN      
        1 cccattcgca catacacttc catctgctcg cggctcggcc caagcacgtc gtcgtcgaac
       61 cgaattcgcc cgcgctgaaa caccgtgatc acgcatgaac cgccgaacgc gaaaagcccc
      121 ttctccgcgc ctttctcgac ggcgcgattc ggcagatacg tttggcgaat gctcccgaca
      181 ttcgtcgcgc ccacctcgaa cattgccacc gtgccaaacg ccgccgactc gatcaacgtg
      241 agcatgcgtt tgttttgcac cagatagtgg aggttgcgcc gcagcgccac cggactcacc
      301 gaatacaaat agccgttgat caaacgtgcc tcgcccggca cgccggcgac cggaaaatga
      361 aaccggtgat agtccaccgg acacaaccgc gagatcacca tcgcgccgcc cgcgaatttc
      421 gccgcgagtt gcggcggccc atccgcgtcc ttcccctcgc caaacaactc cgcgagcgtg
      481 aactgcgcgc ccttcacgta gaaaccgccc gtcgtctcca catcggcaaa cgccagatgc
      541 cgcccgtccg ccggaaacac cgcgacgcgc tcgcccgccg cgatcggacg cgcttcgggt
      601 ttcaacgccc gatagaaaaa ctcgttgaac gttttgtagt cgaaggccga cttcgcgaac
      661 tcgtccacgt cgatgtcgta tttcgtgatg aacggcagga tctgcagcgc gctgacccgc
      721 ttgttcatct ttcgaccata ccaccacgag aaaatctttc gccgcgcaag cagccacacg
      781 aagaagcgcc ccgccggatt gtcgtaggcg aaccgcagcc actcctcgcc gaagattttc
      841 tccgttttca cggctttctt gtagcgatga taaaagcgga tcggctcggt cggcatgggt
      901 gagcgcgaac gttcggcgca aacccgccgc cgcgcaaagc agaaaaccgc tcggcgcgac
      961 gagggcgtcg cggctccaat cgcctgaaaa tggagccgcg gcgccccgcc gcgccttccg
     1021 caccggcggc ttgcctttcg ctctgcgccc taagttcatc tccgcccagt gaaacagagc
     1081 atcgttaccc tcgacatgga aggcgtcctc acgccggaaa tctggatcgc cgtcgccgag
     1141 cgcacgggca tccccgaatt gcgccgcacg acgcgcgacg agccggatta cgacaagctc
     1201 atgacgcagc gcgtcgcgat cctcgcgcaa cacgggatca ccctgtc
//
LOCUS       DASPLT010001010         4085 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_216144, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001010 DASPLT010000000
VERSION     DASPLT010001010.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 4085)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4085)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4085
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_216144"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..546
                     /locus_tag="VEA63_10430"
     CDS             <1..546
                     /locus_tag="VEA63_10430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007361271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HYD84464.1"
                     /translation="VSMPVLAVLWTMLYQPTQDADTGLVNAMLGWLGVAPQAWLRDPS
                     LALPAIAFMSVWQGVGLQMMVFLAGLQNVSAELLDAARIDGANAWRRIVHVVLPAMRN
                     TLVFVITVTTIFAFRIFVQPYLMTRGGPGNSTLSIVQSIYEMTFLSQDLGRACAAAFL
                     FLLLVGAFTLVQRRWAKEERE"
     gene            676..1515
                     /locus_tag="VEA63_10435"
     CDS             676..1515
                     /locus_tag="VEA63_10435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HYD84465.1"
                     /translation="MKQIWMKRPLSWTASIATAILFLAPLLWLVLASLREETRIFRFD
                     ANNWLTTAGWTLQNYADAWRRAALGGGLAASFVQVLLITSVGLLVNAMAAYAFARLAF
                     RGRDALFAVIVILIILPVEVLAVPMFFTARDLGLTGGFTTAIAGLTVPFMAKAFNIYF
                     LRQHFLSLPVELEEAAAIDGASVWRQFWNLALPAVRPALATVVLLDLLVHWGDFLWPL
                     LIATREETRTVQLSLANLFTAPPIQWGDILACAVLATLPVIVLFRWFQRYIVTTDARA
                     GIK"
     gene            1702..2442
                     /gene="pyrH"
                     /locus_tag="VEA63_10440"
     CDS             1702..2442
                     /gene="pyrH"
                     /locus_tag="VEA63_10440"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HYD84466.1"
                     /translation="MHEKPPADPNVKYKRIVLKLSGEVLRGGKSGDPIDGPTLEKICR
                     QVKEISELGVEVCVIIGGGNIFRGLKGAQRGVDRTTGDYMGMLATVINGLALMDCLEK
                     MDVRTRVQSAIPMNQIAEPFILRRAVRHLEKGRVVIFVAGTGNPYFTTDTTAALRASE
                     MHADILMKATKVDGVYDKDPKKYPDAVKYDRITFIDALKQRLNVMDSTAFSLCLDNNV
                     PILVFDLGDEHAIRKAVLGEKIGTLVHG"
     gene            2727..3272
                     /gene="frr"
                     /locus_tag="VEA63_10445"
     CDS             2727..3272
                     /gene="frr"
                     /locus_tag="VEA63_10445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007363002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HYD84467.1"
                     /translation="MSQPLLTDAQTRMKKAVDHTLHEFSAIHTGKATPTMVEGVMVEA
                     YGSSMRLKDCAAISTPDARLIQIQPWDAGLVKAIVKSIQEANLGFNPIPDGKVIRIPL
                     PEMSRERRQEFVKNAHRLAEEGRVHVRNVRRDALEAVKKAKLPEDEAKRVEKDIQTAT
                     DASIKSINDHLAHKEKDLLTV"
     gene            3378..>4085
                     /gene="proB"
                     /locus_tag="VEA63_10450"
     CDS             3378..>4085
                     /gene="proB"
                     /locus_tag="VEA63_10450"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009513402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HYD84468.1"
                     /translation="MTKPANATPLPKRIVIKLGTGVLTTGIGQLDTARIAAVCAEIAA
                     LRARGTEVILVSSGAVGLGMGALQLEKRPKDVSKKQACAAVGQSLLMQHWQSGFAPHD
                     LTVAQVLLTHEDLRGRARFLGVKATLRQLVDYNTIPVINENDTVSAAEIKFGDNDTLS
                     AMVASLVEARYLIILSTAPGLVDLRGTKQIVPVVERITPEIEAMAGGTTSETAVGGMI
                     SKISAAKLATRSGCGVFI"
BASE COUNT          772 a         1370 c         1158 g          785 t
ORIGIN      
        1 gtgtcgatgc cggtcctcgc cgtgttgtgg acgatgttgt atcagccgac gcaggacgcc
       61 gacaccggcc tcgtcaacgc aatgctcggc tggctcggcg tcgcaccgca ggcgtggctg
      121 cgcgatccgt cgctcgcttt gccggcgatt gcattcatgt cggtttggca gggcgtcggc
      181 ttgcagatga tggtgtttct cgccggcctg caaaacgtgt ccgccgaatt gctcgacgcc
      241 gcgcgcatcg acggcgccaa cgcatggcgg cggatcgtgc acgtcgtgct gccggcgatg
      301 cgtaacaccc tcgtcttcgt tatcacggtg acgacgatct tcgcgttccg gatcttcgtt
      361 caaccgtatc tgatgacgcg cggcggtccg ggaaacagca cgctctcgat cgtgcaatcg
      421 atctacgaga tgacgtttct tagccaggat ctcggccgcg cctgcgccgc cgcgtttctc
      481 tttctgctgc tcgtcggcgc gttcacactc gtgcagcggc gctgggccaa ggaggaacga
      541 gaatgaaccg gtccgctctc cgctccgagc tgcgggaggg gttttacgcc ccgacaaacc
      601 gcacattgtt tctttcctgt cttcgaacga cgtcatctcc cttcgaccac atcccgcaaa
      661 cgccgtgaga ttcgcatgaa gcagatttgg atgaaacgcc cgctctcttg gacggcgagc
      721 atcgccaccg ccatcctctt tctcgcgccg ttgctgtggc tggtgctcgc gtcgttgcgc
      781 gaggagacgc gcatctttcg tttcgatgcc aacaactggc tgacgaccgc cggctggacg
      841 ctgcaaaact acgccgatgc atggcgccgc gccgcgctcg gcggcggcct cgccgcgtcg
      901 ttcgtgcaag tgctgctcat cacgagcgtc ggactgctgg tgaacgccat ggctgcgtat
      961 gcgttcgctc gcctggcgtt tcgcggacgc gacgcgcttt tcgcggtcat cgtgatcctg
     1021 atcattctgc ctgttgaggt gctcgccgtg ccgatgtttt tcaccgcgcg cgatctcgga
     1081 ttgaccggcg gtttcacgac ggcgatcgca gggttgacgg tgccgttcat ggcgaaggcg
     1141 tttaacatct acttcctgcg gcagcatttt ctctcgttgc ccgtcgagct cgaggaagcc
     1201 gccgcgatcg acggcgccag cgtgtggcgt caattctgga acctcgcgct cccggccgtg
     1261 cgacccgcgc tcgcgacggt cgttctcctc gatctgctcg tgcactgggg cgatttcctg
     1321 tggccactgc tgatcgccac acgcgaggaa acgcgcaccg tccaactcag tctcgccaac
     1381 ctcttcaccg cgccgccgat tcaatggggc gatatcctcg cgtgtgccgt cctcgccacg
     1441 ctgccggtga tcgtgctctt tcgctggttt cagcgctaca tcgtcaccac tgacgcccgc
     1501 gccgggatca agtagccggc ccgcttccca tgtgggtccg cgagcagcgt tcaccatcct
     1561 cgcgctcaaa cggcgcgtgc agcccgcgac ccgcctaaga gattctccga tgcgccggtt
     1621 gcgcggtgga gcggacaatc ccacaaacgc ccaaccagcg cgggattgac gcggattttc
     1681 gggcgtcgtt tcctgtcctt catgcacgaa aagccgccgg ctgatccgaa cgttaagtat
     1741 aaacgcatcg tcctcaaatt aagcggcgag gtgcttcgcg gcggaaaatc cggcgatccc
     1801 attgacggcc ccacgctcga gaaaatctgc cggcaggtga aagaaatcag cgaactcggc
     1861 gtcgaggtct gcgtgatcat cggtggcgga aatatttttc gcggcctcaa aggcgcgcag
     1921 cgcggcgtcg atcgcacgac cggcgactac atgggcatgc tcgcgaccgt gatcaacggc
     1981 ctcgcgctca tggattgcct cgagaaaatg gacgtgcgca cgcgcgtcca aagcgcgatc
     2041 ccgatgaacc agatcgccga gccgttcatc ctccgccgcg ccgtccgcca cctcgaaaaa
     2101 ggccgcgtcg tcatcttcgt cgccggcacg ggcaatcctt atttcaccac cgacaccacc
     2161 gccgcgctcc gcgcgtccga gatgcacgcc gacatcctga tgaaggccac gaaagtcgat
     2221 ggcgtttacg acaaagaccc caagaaatat cccgacgcgg tgaaatacga ccgcatcacg
     2281 ttcatcgacg cgctcaagca gcggctcaac gtcatggact cgacggcgtt ttccctctgc
     2341 ctcgacaaca acgtgccgat cctcgtcttc gatctcggcg acgagcacgc cattcgcaag
     2401 gccgtcctcg gcgagaagat cggcacactc gtgcacggct aagccggagt catgcagtag
     2461 aaaccacgaa tggacacgaa ttcacacgaa taccagagga gctcgaccga agtatctgag
     2521 accgtcgcgc gacaaccaaa ccgtggcttt attgcgacca ccggctagat cgggttttca
     2581 ttcgtgtctc ttcgtgtcca ttcgtggttc aattgaattg ctacggctca gcgttcaaac
     2641 tccgcgcaga gcgtcgcaca cgcttgcggc ctttgccgac ggctcttagc tctggattca
     2701 gcgcatagac taaactccac cctcccatgt cccaacccct tctcaccgac gcccagactc
     2761 gcatgaaaaa agccgtggat cacacgctgc atgaattcag cgcgatccac accggcaaag
     2821 cgacgcccac gatggtcgaa ggcgtgatgg tcgaagccta tggttcctcg atgcgcctca
     2881 aggattgcgc cgcgatctcc acgcccgacg cccggctcat ccagattcag ccgtgggacg
     2941 ccggcctcgt gaaagccatc gtcaagtcca tccaggaggc gaacctcggc ttcaacccga
     3001 tccccgacgg aaaagtcatt cgtattccgc tgccggaaat gagccgcgaa cgccggcagg
     3061 aattcgtcaa gaacgcccat cgcctcgccg aggaaggccg cgtccacgtg cgcaacgttc
     3121 gccgcgacgc cctcgaggcc gtcaagaaag cgaaacttcc cgaggacgag gcgaagcgcg
     3181 tcgaaaaaga tatccagact gcgaccgatg cctcgatcaa gagcatcaac gatcatctcg
     3241 cgcacaagga gaaggacctg ctcacggtgt aaaaactcct aatcccaaaa tccgagctcc
     3301 agagaatcct caattcctgg agcctgattc ttggagattc cgtggagttt ggatcttggt
     3361 gcttggagct tagccctgtg acgaaacccg cgaacgccac gcccctcccc aaacgcatcg
     3421 tcatcaaact cggcaccggc gtcctgacca ccggtattgg ccagcttgat acggcgcgca
     3481 tcgccgccgt ttgcgcggag atcgccgcgc ttcgcgcccg cggcaccgag gtcatcctcg
     3541 tgagctccgg cgccgtcggc ctcggcatgg gcgcgctcca gctcgagaaa cggccaaagg
     3601 acgtttcgaa aaaacaggcg tgcgccgccg tcggccagag cttgctgatg caacactggc
     3661 agtccggttt cgctccgcac gacctcaccg ttgcgcaggt tctcctcacg cacgaggacc
     3721 tgcgcggccg cgcgcgcttc cttggcgtca aagccacgct gcgccagctc gtcgactaca
     3781 acaccattcc ggtcatcaac gaaaacgaca ccgtcagcgc cgccgaaatt aaattcggcg
     3841 acaacgacac gttgtccgcg atggtcgcga gcctcgtcga agcgcgttac ctcatcattc
     3901 tctcgaccgc gcccggcctc gttgatttgc gcggcacgaa gcaaatcgtt cccgtcgtcg
     3961 aacgcatcac gcccgagatc gaagcgatgg ccggtggcac caccagcgag accgccgtcg
     4021 gcggcatgat ctcgaaaatc tccgcggcca agctcgcgac gcggtccggc tgcggtgtgt
     4081 tcatc
//
LOCUS       DASPLT010001011         3010 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_12685, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001011 DASPLT010000000
VERSION     DASPLT010001011.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 3010)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3010)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3010
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_12685"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..287)
                     /gene="ilvN"
                     /locus_tag="VEA63_10455"
     CDS             complement(<1..287)
                     /gene="ilvN"
                     /locus_tag="VEA63_10455"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HYD84469.1"
                     /translation="MRHTISVLVENKFGVLARVSGMFSGRGFNIDSLNVAPTHDASLS
                     RITAVLNGDEVQLDLCIKQLRKLINVVDVIDFKEGQAVARELVLVKVRATA"
     gene            complement(315..391)
                     /locus_tag="VEA63_10460"
     tRNA            complement(315..391)
                     /locus_tag="VEA63_10460"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(355..357),aa:Val,seq:tac)
     gene            complement(534..1637)
                     /locus_tag="VEA63_10465"
     CDS             complement(534..1637)
                     /locus_tag="VEA63_10465"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HYD84470.1"
                     /translation="MPRGRQVLVMSSNGVSMSNSPSLTVGIVGATGAAGQELLRLLHE
                     RNFPMASLRLFASARSAGKVVEVAGKKYTVEEAKAGVFGDVDVAFFATEGAITKALAP
                     DAVKAGCVVIDKSSAFRMDPSVPLVIPEINPEGLRQHNGIIANPNCSTAVLLMALWPL
                     HQAFGLKRYFAATYQSVSGTGVDAVEELETQIHAYVKGGTLQNKVYPYQIAFNVIPQV
                     DSFGNDGYTGEETKMSQESRKIMGLPNLKVSSTCVRVPVVQAHSIAVNAEFERPVSVE
                     AARNAIAEFEGAELVDDPANKRYPTPLDFTRKVKCGVGRVRLDTALDNGLALWVSGDN
                     LWKGAALNALQNAELMVREGWLKPKRALAAVAG"
     gene            1588..2124
                     /locus_tag="VEA63_10470"
     CDS             1588..2124
                     /locus_tag="VEA63_10470"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007359249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HYD84471.1"
                     /translation="METPLELITKTCLPLGIKPTDRFLFVRIGTATLQFFRNGVLIRS
                     YAVSTSKRPPSNVKNSLGTPRGLHEIAERIGAGQPPGTVFKSRVATGHHYSELSNAAA
                     QDNLITTRILWLRGLEPGVNRGGDVDTYDRYIYLHGTNHEHRIGEPISAGCVLMRNLD
                     LVELYEQVRTGDHVLIVD"
     gene            2246..2425
                     /locus_tag="VEA63_10475"
     CDS             2246..2425
                     /locus_tag="VEA63_10475"
                     /inference="COORDINATES: protein motif:HMM:NF017353.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HYD84472.1"
                     /translation="MKASTRNQAAGHKNVAAGKTKATAGKVTGNRSLQAKGRAQEIAG
                     KIQKKVGARQKVRGA"
     gene            complement(2669..>3010)
                     /locus_tag="VEA63_10480"
     CDS             complement(2669..>3010)
                     /locus_tag="VEA63_10480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009510727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-transporting P-type ATPase"
                     /protein_id="HYD84473.1"
                     /translation="VAMVGDGVNDAPSLAAADVSVAMGARGSDAALEQAEVILMHDRI
                     ENFLAAQRLSRRAQRVIRQNLTISLGVVIVMVVATAWGAVPLAIGVAAHEGSTLVVCL
                     NSLRLLFGRNR"
BASE COUNT          637 a         1094 c          770 g          509 t
ORIGIN      
        1 tgcgccgtcg cgcgcacctt taccaacacc agctcgcgcg ccaccgcctg accttccttg
       61 aagtcgatca cgtcgacgac gttgatcagc ttccgaagct gcttgatgca aagatcgagc
      121 tgcacctcgt cgccgttcaa caccgccgtg atccgcgaaa gcgaggcatc gtgcgtcgga
      181 gcgacgttga gcgaatcgat gttgaagccg cgcccggaaa acattccgga gacgcgcgcc
      241 aaaacgccaa acttgttctc aacgaggacg gagatcgtat gtcgcatgaa agtgcagatt
      301 gaggtataaa aacgtggtgg acgctgaggg actcgaaccc ccgacaccct cggtgtaagc
      361 gaggtgctct aaccaactga gctaagcgtc cgagccgagg ggcgataaaa gccgccgcca
      421 cccgcgtatg acaaggaatt attccggtat gacttgggtc tgaaaaaaga aggaaaaggc
      481 ggctcgacca gccgccttga gttaagtcac cgcattcagc cgccgtccgc gtctcaccca
      541 gccactgccg cgagcgctcg cttcggcttc agccagcctt cgcgcaccat cagctcggcg
      601 ttctgcagcg cattcaaagc cgcgcctttc cagagattgt cgccgctcac ccagagcgcg
      661 aggccgttgt cgagcgccgt atccagacgg acacgaccca cgccgcactt cactttgcgc
      721 gtaaaatcga gcggcgtcgg ataacgcttg ttcgccggat cgtcgaccag ctccgcgcct
      781 tcgaattccg cgatcgcatt ccgcgccgcc tcgacgctca ccggtcgctc gaactccgca
      841 ttcaccgcga tcgagtgcgc ctgcacgacc ggcacgcgca cacaagtcga cgacactttg
      901 aggttcggca gacccatgat cttgcggctc tcctgcgaca tcttcgtctc ctcccccgtg
      961 taaccgtcgt taccgaacga atccacctga gggatcacgt tgaacgcgat ctgatacgga
     1021 taaactttgt tctgcagcgt cccacccttc acgtaagcgt gaatctgcgt ctcgagctcc
     1081 tcgaccgcat cgacgcccgt gcccgacacc gactgatacg tcgccgcgaa ataacgcttc
     1141 agaccgaacg cctggtgcag cggccagagc gccatcaaca acaccgccgt cgagcaattc
     1201 ggattcgcga tgatgccgtt gtgctgccgc agcccttccg ggttgatctc cgggatcacg
     1261 agcggcaccg acggatccat ccggaacgcc gagctcttgt cgatcaccac gcaacccgcc
     1321 ttcaccgcgt ccggagcgag cgccttcgtg atcgcacctt ccgtcgcgaa aaatgccacg
     1381 tcgacgtcgc caaacacgcc cgccttcgcc tcctcgaccg tgtatttttt gcccgccacc
     1441 tcgacgacct tgcccgccga ccgcgccgag gcgaagagcc gcagcgaagc catggggaaa
     1501 tttcgctcgt gcaagagccg cagcagctct tgaccggcgg cgcccgtggc accgacgata
     1561 ccgactgtaa gggatggcga attactcatg gaaactccgt tggaactcat caccaaaacc
     1621 tgtctgcccc tcggcatcaa gccaacagac cgtttcctct tcgtgcgcat cggcacagcg
     1681 acgctccaat ttttccgcaa cggtgtgctc attcgcagct acgccgtgtc cacctccaaa
     1741 cgcccgccct ccaacgtgaa gaactccctc ggcaccccgc gcggccttca cgaaatcgcc
     1801 gaacgcatcg gcgccggcca gccgcccggc accgtgttca aatcccgcgt cgccaccggc
     1861 catcactact ccgagctctc taacgccgcc gcgcaggaca atctgatcac cacgcgcatc
     1921 ctctggcttc gcggcctcga acccggcgtc aaccgcggtg gcgacgtcga tacttacgat
     1981 cgctacatct accttcacgg cacgaatcac gagcaccgca tcggcgagcc gatcagcgcc
     2041 ggctgcgtcc tcatgcgcaa cctcgacctc gtcgaactct acgaacaagt ccgcaccggc
     2101 gatcacgtgt taatcgtcga ctgagccacg cgtattcgtt ttcccacgac accccgccgc
     2161 gcgtccgctc gggaaccgcc acgccccact ccggtcggtg atagcgcgtg acgtcatcgt
     2221 cacgaaaaaa cccaacccca aaaaaatgaa agcaagcaca cgcaaccaag ccgccggtca
     2281 caaaaacgtc gccgccggca agacgaaagc gaccgccggc aaagtcaccg gcaaccgctc
     2341 ccttcaagcc aagggacgcg cgcaggaaat tgcgggcaag atccagaaga aagtcggcgc
     2401 ccgccaaaaa gtccgaggcg cctgatcgac tcgtctcggc gtcattcaac cacggccggc
     2461 cgccccgcgc gccggccttt ttgttgtcct gacgcgcacg ttcgcaccag gccccgtgct
     2521 aaaaggaatc cgaacgaaac gcccgtgcgt cgccgattag gcgaagcacg cactcattcg
     2581 cattcacgac cgtcctccga aaatcagcgt aaattagcgg ttcaaaacct ccgaccttga
     2641 tcgtcaggtt caatcaggct ctacgatttc aacgattccg cccgaaaagc agtcgcaacg
     2701 aattcaaaca caccacgagc gtgctgcctt catgcgctgc cacgccgatt gcgagcggca
     2761 ccgcgcccca cgccgttgcc accaccatca cgatcaccac gcccagcgag atcgtcagat
     2821 tctgccggat cacccgctgc gcacggcggc tcaatcgctg cgccgccaga aaattctcga
     2881 tgcggtcgtg catcagaatc acctccgcct gctcgagcgc cgcatcgctc ccgcgcgcgc
     2941 ccatcgccac gctcacgtcc gccgccgcca agctcggcgc atcgttcacg ccgtcgccca
     3001 ccatcgccac
//
LOCUS       DASPLT010001012         1043 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_257820, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001012 DASPLT010000000
VERSION     DASPLT010001012.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1043)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1043)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1043
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_257820"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1043
                     /locus_tag="VEA63_10485"
     CDS             <1..>1043
                     /locus_tag="VEA63_10485"
                     /inference="COORDINATES: protein motif:HMM:NF019259.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1592 domain-containing protein"
                     /protein_id="HYD84474.1"
                     /translation="RSFAESDSPLQQLTPRKGQDAVPSVRDFEIYGPLNPAGLSDTPS
                     RQKIFSCHPDRDAAELTCARQIVTSLANEAFRGFLTDEDTELLMKMYDVGATEGGFEK
                     GVSFALSGILAHPKFLYRVEPVPGSVPPGVSYELTSLELASRLSFFLWSSVPDETLRE
                     IAVDDGLKDPVVLEAQVKRMLRDPRAANLANNFGFQWLGLAELDNINPDGALFRDVDR
                     NIRSDLTEEALRFVQSIFEEDRSVVDLLTADHTYLNESLALHYGINNVRGGEFRRVQL
                     DDERRFGLLGKGAVLMVSSYPNRTSPVLRGKWLMEKIIGTPPTPPPPDVEGFVEIEPG
                     QEFTTVRQRLEEH"
BASE COUNT          198 a          347 c          317 g          181 t
ORIGIN      
        1 atcgttcgtt tgccgaatcc gattcgccgc tgcagcagct gacgccgcgc aagggccagg
       61 atgcagtgcc gtcggtgcgc gatttcgaaa tctacggacc gctgaacccg gccggcctca
      121 gcgacacgcc gagccgccag aaaattttct cgtgtcatcc cgatcgcgac gcagcggaac
      181 tcacctgcgc gcgccagatc gtcacgtcac tcgcgaacga agcgttccgc ggtttcctga
      241 ccgacgaaga caccgagctg ctgatgaaga tgtatgacgt cggcgccacc gaaggtggtt
      301 tcgagaaagg cgtcagcttc gcgctgtcgg gtatcctcgc gcatccgaag ttcctgtatc
      361 gcgtggagcc cgtgcccggt tctgtgccgc ccggcgtgtc gtatgaactg acgagcctcg
      421 agctcgcgtc gcgcctgtcg ttcttcctgt ggagctcggt gcccgatgaa acgctgcgcg
      481 aaatcgccgt cgacgacggc ctgaaggatc cggtcgtgct cgaagcacag gtcaagcgca
      541 tgctgcgcga tccgcgcgcg gcgaacctgg cgaacaactt cggcttccag tggctcggtc
      601 tcgccgagct cgacaacatc aatcccgatg gcgcgctgtt ccgcgacgtc gaccgcaaca
      661 ttcgcagcga cctgaccgag gaagcactgc gcttcgtgca gagcatcttc gaagaagacc
      721 gcagcgtcgt cgacctgctg accgccgatc acacctactt gaacgaaagc ctcgcgctgc
      781 actacggcat caacaacgtg cgcggcggcg aattccgccg cgtgcagctc gacgacgagc
      841 gccgcttcgg tctgctcggc aagggcgccg tgctgatggt gtcgtcgtat ccaaaccgca
      901 cgtcgccggt actgcgcggc aaatggctga tggaaaaaat catcggcacg ccgcccacgc
      961 cgccgccgcc ggatgtcgaa ggcttcgtcg aaatcgaacc gggccaggaa ttcacgaccg
     1021 tgcgccagcg cctcgaggaa cat
//
LOCUS       DASPLT010001013         1666 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_257835, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001013 DASPLT010000000
VERSION     DASPLT010001013.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1666)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1666)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1666
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_257835"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..966
                     /locus_tag="VEA63_10490"
     CDS             <1..966
                     /locus_tag="VEA63_10490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HYD84475.1"
                     /translation="YVKSGAASFDLIALRTALTAQPLTDTLQNNIFALLMFGFGILVS
                     LWPLHTWAPLGYGAAPSSNAMLHAGVLKKFGLYGLIQIALPLLPAGFAHWSGPLAWLA
                     VIGNVLIVGFITMAQRDLKQMVGYSSVMHMGYAFLGIAAGTTLGIGGVVLMMVAHGFS
                     VALLFLLSTSIYHRTHTFAMDEMGGLAQKTPVLAAFFVAATFASIGLPGFANFWGELT
                     IFIALWEFSPLVTALAIAGIIISAIYGLRAAANVFFGQPSEALTKMMAQHPPLDLRWS
                     EKIPALILLAALLFVGFWPKSISTQIDAALADAVPVQTMETTNSR"
     gene            1099..>1666
                     /locus_tag="VEA63_10495"
     CDS             1099..>1666
                     /locus_tag="VEA63_10495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HYD84476.1"
                     /translation="MNLEAVKAAALANEWAAIAPELALGCLALLLLIVEMLLPKKQHA
                     HIATLSIVGQLGILAAVAVNFHSPFLGRDTFNGLLHHGYIGQSLRVFFLLSSIFVSIL
                     ARVSLAKQVLPRVEFHHIVLVVTGAMMLLAQANHFVLLFVALETLTIGLYILVSYFRA
                     NALSLEAGLKYLVMGALSSSLLLFGIVLL"
BASE COUNT          254 a          600 c          439 g          373 t
ORIGIN      
        1 tacgtgaaaa gcggcgccgc atccttcgac ctcatcgcgc tgcgcaccgc gctcaccgcg
       61 caaccgctca ccgacacgct tcaaaataac atcttcgcgc tgctgatgtt cggcttcggc
      121 atcctcgtct cgctctggcc gctgcacacc tgggcaccgc tcggctacgg cgccgcaccc
      181 agttcgaacg cgatgcttca cgccggcgtg ctaaaaaaat tcggcctcta cggcctcatc
      241 cagatcgcgc tcccgctgct gcccgccggc ttcgctcact ggtccggtcc gctcgcgtgg
      301 ctcgcggtca tcggcaacgt tctcatcgtc ggcttcatca cgatggcgca acgcgacctc
      361 aagcagatgg tcggttacag ctcggtgatg cacatgggtt atgcctttct cggcatcgcc
      421 gccggcacga cgctcggcat cggcggcgtc gtgttgatga tggtcgcgca cggtttttcg
      481 gtcgcgctcc tgtttctgct ttcgacgtcg atctaccacc gcacgcacac gttcgcgatg
      541 gacgagatgg gcggcctggc gcagaagacg cccgtcctcg ccgcgttctt cgtcgcagcg
      601 accttcgcga gcatcggcct tccgggcttc gcgaacttct ggggcgaact cacgatcttc
      661 attgcgctgt gggaattctc gccgctcgtc accgcgctcg cgatcgccgg cattatcatc
      721 tcggcgatct acggcctccg cgccgccgcc aacgtgttct tcggccagcc gtccgaggcg
      781 ctcacgaaga tgatggcgca acatccgccg ctcgatctgc gctggtccga gaagattccc
      841 gcgctcatcc ttctcgccgc gctcctcttc gtcggcttct ggccgaagtc gatctccact
      901 cagatcgacg cggcgttggc cgacgccgtt ccggttcaaa ccatggaaac cactaattca
      961 cgctaatgac cgctaatttc caaaccacgg aagcaggctc ccacctcaag ccactcttca
     1021 ttagcgtcca attagcgcag atcagcggtt aactcgtctc cgttctccgt tttctgcctc
     1081 cgccttccgt ctctccgaat gaatctcgaa gccgtcaaag ccgccgccct tgcgaacgag
     1141 tgggccgcca tcgcgcccga actcgcgctt ggctgcctgg cgctgctcct cctcattgtg
     1201 gaaatgctgc tcccgaaaaa gcagcacgcg cacatcgcca cgctttcgat cgtgggccag
     1261 ctcggcattc tcgccgccgt cgcggtgaac ttccattcgc cgttcctcgg tcgcgacacg
     1321 ttcaacggcc tgctgcacca cggctacatc ggccagtcgc tgcgcgtgtt cttccttctg
     1381 tcgtcgatct tcgtcagcat tctcgcgcgc gtcagtctcg cgaaacaggt gctgccgcgc
     1441 gtcgagtttc accacatcgt gctcgtcgtc accggtgcga tgatgctgct cgcgcaagcg
     1501 aaccacttcg ttctgctctt cgtcgcgctc gagacgctca cgatcgggct ctacatttta
     1561 gtcagttatt tccgcgccaa cgcgctctcg ctcgaagccg gcctgaagta tctcgtcatg
     1621 ggcgcgctca gctcctcgct gctgctcttc ggcatcgtgc tgctct
//
LOCUS       DASPLT010001014         1034 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_197358, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001014 DASPLT010000000
VERSION     DASPLT010001014.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1034)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1034)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1034
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_197358"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1004)
                     /locus_tag="VEA63_10500"
     CDS             complement(<1..1004)
                     /locus_tag="VEA63_10500"
                     /inference="COORDINATES: protein motif:HMM:NF014850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HYD84477.1"
                     /translation="MSYAAVWLNGHIVGGWPFGYSSWRVDLTPYVKFGAENQIAIRLD
                     NPPASSRWYPGGGIYRNVWLTKTAPVRVTQWGTRVTTRDISPAAATIAFEVEVENASA
                     QSAEVDVVTEIFELDARGRVRGAAVAKIDAIRARISAGGNAKVNGSVVLSQPKLWGPP
                     PTQQPHRYVAVTKVSRDGKSVDVYETRLGIRSLRFDADSGIYVNGERIQIKGVNQHHD
                     LGALGAAFNVRAAERQLEILREMGCNSIRMAHNPPAPELLELTDRMGFLVVNESFDVW
                     KRRKTPLDFHLIYPDWHEQDMRALVRRDRNSPSVIMWSIGNEVGEQYTGNEGAAVARR
                     "
BASE COUNT          191 a          336 c          306 g          201 t
ORIGIN      
        1 aaccgccgcg cgaccgccgc gccttcattg ccggtgtatt gctcgccgac ttcgttgccg
       61 atgctccaca tgatgacgga agggctgtta cgatcgcggc gaaccagcgc gcgcatgtcc
      121 tgctcgtgcc agtcggggta aatcaggtga aaatcgagcg gtgttttccg tcgcttccac
      181 acatcgaacg attcgttcac cacgagaaat cccattcgat ccgtgagctc gagcaactca
      241 ggcgccgggg gattgtgcgc catgcgaatc gaattgcagc ccatctcgcg gagaatctcc
      301 aactggcgct cggcggcacg gacgttgaac gctgcgccga gcgcaccgag gtcgtgatgc
      361 tgattcacgc ctttgatttg gatgcgctcg ccattcacat aaatgcccga gtccgcatcg
      421 aagcgcagcg agcgaatgcc gaggcgcgtt tcataaacgt cgaccgattt tccgtcgcgg
      481 gaaactttcg tcacggcgac gtagcgatgc ggctgctgcg tcgggggcgg cccccagagc
      541 ttcggttgcg agagcacgac ggagccgttc accttcgcat ttccgcccgc ggaaatcctg
      601 gctctaatcg cgtcgatctt cgccaccgcg gcgccgcgca cgcggcctcg cgcatcgagt
      661 tcaaaaatct ctgtgaccac atcgacttcc gcactctgcg ccgacgcgtt ctccacttcg
      721 acttcgaacg cgattgtcgc cgcggccggc gagatgtcgc gcgttgtcac gcgcgtgccc
      781 cactgcgtga cgcgcaccgg cgcggttttc gtgagccaca cattgcggta gattccgccg
      841 ccgggatacc aacgcgatga cgccggcgga ttatcgaggc ggatcgcgat ttggttttca
      901 gcgccgaact tcacgtaggg cgtgaggtcg acgcgccacg acgaataccc aaacggccaa
      961 cccccgacga tgtgtccatt gagccagacc gccgcgtagg acatcgcgcc gtccacttcg
     1021 aggaagatcg attt
//
LOCUS       DASPLT010001015         1129 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_51659, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001015 DASPLT010000000
VERSION     DASPLT010001015.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1129)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1129)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1129
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_51659"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(214..606)
                     /locus_tag="VEA63_10505"
     CDS             complement(214..606)
                     /locus_tag="VEA63_10505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD84478.1"
                     /translation="MALLVILGVALSCFAAGSQAVVFHGLVTNTTAAASANVRLVLTI
                     DGENVSGEMTTEPPLSGAGKLEGRLRGGWCELSGKLNEGFTIQFRGAFNGRDFRGTYV
                     VAVPGEFVQYGKHHLKLHPVAKTMAPAK"
     gene            complement(645..>1129)
                     /locus_tag="VEA63_10510"
     CDS             complement(645..>1129)
                     /locus_tag="VEA63_10510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD84479.1"
                     /translation="PELRALGRLLKKFSEMPVDQIRIAGQRTAAGEVELSELRIDSPQ
                     ARLLARGRIPAVDGEPLMNRPLELSVDLAAKEEMAVILGGMSLIESKPREDGYRPLKQ
                     KFILGGKAGAPDTRPLYDLLAQAVGGSKGTWGFLMRRVQNQLNKMKPAAPKKTAKTSQ
                     "
BASE COUNT          208 a          380 c          313 g          228 t
ORIGIN      
        1 ggaggcgaca ttccaaaagc tcggcgttga aaaccgcacc tcggccgccg cggtggcttt
       61 ggagaaatta accggctaaa agctgccgcg ttgtaggaac agtgccgcat atcgatgccg
      121 gtggcaggcc gcgctcctgc accgcgcctt cgctctctct tgcgttgcgg ggggaattct
      181 cgagcgactg ccttgtttca tctgccaccg acttcacttg gcgggcgcca tggtcttcgc
      241 cacgggatgc agtttcaggt ggtgcttccc gtattgcacg aactcacccg gaaccgcgac
      301 gacataggtg ccgcgaaaat cccgtccatt gaacgcgcct cgaaattgga tcgtgaagcc
      361 ttcgttcagt ttaccactca gctcgcacca gccaccacgc agcctgcctt cgagtttgcc
      421 cgcacctgac agcggcggct cggtcgtcat ctctccgctg acgttttcgc cgtcaatcgt
      481 gagcacgagc cgcacgttgg ccgatgccgc ggccgtcgtg ttcgtcacga gaccatgaaa
      541 taccacggcc tgtgaaccag ccgcgaaaca cgacagcgcg actcccagaa tgaccagcag
      601 cgccacgcgg gcgcccagcc gtagtcgttt gcaggcgggc aacatcattg cgaggtcttc
      661 gcggttttct tcggcgccgc tggcttcatt ttattcaact gattttgcac ccgccgcatc
      721 agaaaacccc acgtgccttt tgagccgccg acggcttgcg ccagcaaatc atagagcggc
      781 cgcgtgtcgg gtgcgccggc tttcccgccg agaatgaatt tttgcttcag cgggcgatag
      841 ccgtcttcgc gcggcttgct ttcgatcaaa ctcatgccgc ccaggatcac cgccatttct
      901 tccttcgccg cgagatcgac cgaaagttcg agcgggcggt tcatcaacgg ctcgccgtcc
      961 acagccggaa tccgaccacg ggccaacagc cgcgcctgtg gcgaatcgat ccgcagctca
     1021 ctcaactcaa cttcacccgc tgcggtgcgt tgccccgcga tgcggatctg atccacgggc
     1081 atctcggaaa atttcttcaa caaccgcccg agcgcacgca gctcgggcg
//
LOCUS       DASPLT010001016         1171 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_51666, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001016 DASPLT010000000
VERSION     DASPLT010001016.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1171)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1171)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1171
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_51666"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..940)
                     /locus_tag="VEA63_10515"
     CDS             complement(<1..940)
                     /locus_tag="VEA63_10515"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HYD84480.1"
                     /translation="MLKKFLIAIGGFIAVVLVLGAIKASQVNQMMSAPHAAPLASVST
                     TEAKPVNWHASIHSVGTLAPVEGVTISADADGIVVKLGADSGAAVKAGDLLVALDTSV
                     EEAQLAAAEARAELARINIERTRELFKSEAISKSEFDAASASIKQADAEVSGLRAMIA
                     KKQVRAPFDGRVGIRLVNLGQFVAKGAPLMPLQKLDPIYINFNVPQRQLPALSIGQKI
                     SVIVDAFESTPFEATVTAINSQVDPATRNVSVQGTVANPSEQLRAGMFARVEVQMVEA
                     DSLVVVPSTAISYASYGNSVFIVETMKGEDGQEFLGV"
     gene            complement(987..>1171)
                     /locus_tag="VEA63_10520"
     CDS             complement(987..>1171)
                     /locus_tag="VEA63_10520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HYD84481.1"
                     /translation="TPKGQALLETILPPHFKRMAALMNPLTETERKTLVDLLKKIRNQ
                     AAGMPAPEEPRCSAAS"
BASE COUNT          211 a          369 c          362 g          229 t
ORIGIN      
        1 ggacgccgag aaactcctgg ccgtcctcgc ccttcatcgt ctcgacgatg aatacggagt
       61 tgccgtaaga cgcataggag atggccgtcg acggcacgac gacgagcgaa tcggcttcga
      121 ccatctgcac ttcaacgcgc gcaaacatgc cggcgcggag ttgctcgctc ggattcgcca
      181 ctgtgccctg cacggaaacg ttgcgcgtgg ccggatccac ttgcgagttg atcgccgtca
      241 cggtcgcttc gaacggcgtg ctctcgaatg catcgacgat gacggaaatt ttctgcccga
      301 tcgaaagcgc cggcagctgc cgctgcggca cgttgaagtt gatgtagatc ggatcgagtt
      361 tctgcagagg catcagcggt gcgcccttcg cgacgaactg gccgagattg acgaggcgaa
      421 tgcccacgcg tccgtcgaac ggcgcacgca cctgcttctt cgcaatcatg gccctcaatc
      481 cgctgacttc cgcatccgcc tgcttgatcg acgccgaggc ggcgtcgaac tcggacttcg
      541 aaatcgcctc gcttttgaac agctcgcggg tgcgctcgat gttaatgcgc gcgagttcgg
      601 cgcgcgcctc ggcagcggcg agttgcgcct cttcgacgct cgtgtcgagc gccaccagca
      661 aatcgcccgc ctttaccgcc gcaccgctgt cggcgccgag cttgaccacg atgccgtccg
      721 cgtcggcact gatcgtcacg ccttcaacgg gcgcgagcgt gccgacggaa tggatcgacg
      781 catgccagtt cacgggcttg gcttccgtgg tcgacaccga agcaagaggc gccgcgtgcg
      841 gggccgacat catctgatta acttggctgg ctttgatcgc gccgagcacg agaacgacgg
      901 caatgaagcc gccgatggcg atgaggaact tcttaagcat aggaggacag ggttggtgtg
      961 agttggtcga aactgaaagg tgtgcgtcag ctcgccgccg agcatcgcgg ttcttccggc
     1021 gccggcatcc ccgccgcctg gttgcgaatc ttcttcaaca gatcgacgag cgtctttcgc
     1081 tccgtctccg tcagggggtt catgagcgcc gccatccgtt tgaaatgcgg cggcaaaatc
     1141 gtctcgagca acgcctgccc ttttggggtc a
//
LOCUS       DASPLT010001017         1265 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_277791, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001017 DASPLT010000000
VERSION     DASPLT010001017.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1265)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1265)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1265
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_277791"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            240..722
                     /locus_tag="VEA63_10525"
     CDS             240..722
                     /locus_tag="VEA63_10525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD84482.1"
                     /translation="MRFSRLGSVLFCLAIIGSSLARAADIEFVRVWPGWRDAADFESI
                     SEYFSGRENTGRRVVLRSQADARAGFYYLVRVMNSAAPQTGAKFSLQVIAPASPDVKT
                     YAFPADIPARATVFQLGLTGADWPNKETHPVAWKLDLLDAKGQVLASQQSFLWAMPAK
                     "
     gene            806..>1265
                     /locus_tag="VEA63_10530"
     CDS             806..>1265
                     /locus_tag="VEA63_10530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009513254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA glycosylase"
                     /protein_id="HYD84483.1"
                     /translation="MSDEPPLTPGILREILDGGQAFRWQVQPDGTWSGVFGSHVVRLA
                     LAASGTLLWSAPAVSAARTHRALHAYWDSQHTARDLADALPWRSDAHLAACIEAFPGL
                     RILRQPFGETLLGFLCSATKQIVQIKQMLALLAERHGAPIDDGIARRLPTW"
BASE COUNT          203 a          439 c          379 g          244 t
ORIGIN      
        1 ctgtaagcag gccgatgtct tctgctcgcc acgatctcgt gcgtttccgc cacaactgcg
       61 gacctcgccg accaccgtcc accgcacgaa aaaacgttct tactttcctc ccagcaaaaa
      121 cggcgggttt ttcagaggct ccttaaatga caaatccgcg accattgccg ccgggggcgc
      181 gggggcgcgt ggcggtcgaa aaacttgtca ctgggacgac ggcaaatcac gttgtggcga
      241 tgcgtttctc ccgcctgggt tccgttctct tctgccttgc gatcatcgga tcttcgctcg
      301 cccgcgctgc ggatatcgag ttcgtccgcg tctggccggg ctggcgcgat gccgccgact
      361 tcgaaagcat ctccgaatac ttcagcggac gcgaaaacac cggccggcgc gtcgttctgc
      421 ggtcgcaagc cgacgctcgc gctggatttt actatttggt ccgcgtcatg aattccgcgg
      481 cgccgcaaac cggtgcgaag ttttcactcc aggtcatcgc gccagcctcg cccgatgtta
      541 aaacttacgc gtttcccgcc gatatccctg cgcgcgcgac agtgtttcag cttgggctca
      601 ccggagccga ctggcccaac aaggagacgc atccggtcgc atggaaactc gacctgctcg
      661 acgcgaaagg ccaggtgctc gccagccagc agagcttcct ctgggcgatg cccgcgaagt
      721 gacgcgcacc gctcgcagcc agagcgccga gagcgcacca agcgccggcc caccgcccgt
      781 gcccggcttc tccgcatggc gatccttgtc ggatgagccg ccgctcacgc ccggcattct
      841 gcgcgagatc ctcgacggag gtcaggcgtt tcgctggcag gtccagcccg atggcacatg
      901 gtccggcgtt ttcggttcgc acgtcgtccg gctcgcgctc gctgcttcgg gaacgctgct
      961 ctggtcggct cccgccgtct cggctgcgcg aacccaccgc gcgctgcacg cgtattggga
     1021 ttctcaacac actgctcgcg atctcgccga cgctctgccg tggcgcagcg atgcgcatct
     1081 cgcggcgtgc attgaggcct tccccggcct gcggattctg cgccagccgt ttggcgagac
     1141 attgctggga tttctctgca gcgccaccaa gcagatcgtg caaatcaagc agatgctcgc
     1201 gctcctggcc gagcggcatg gcgcgccgat cgacgacggc atcgcgcggc gactgccgac
     1261 ctggg
//
LOCUS       DASPLT010001018         3314 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_12773, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001018 DASPLT010000000
VERSION     DASPLT010001018.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 3314)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3314)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3314
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_12773"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..950)
                     /locus_tag="VEA63_10535"
     CDS             complement(<1..950)
                     /locus_tag="VEA63_10535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HYD84484.1"
                     /translation="MIIAALALATASPALGSADDATLSPLNVRGLPFTRSYPLEEVGE
                     MPRGARLNFDQFGRLAVIYAGFYAVLNDTVWLDIAVKDEPLRVNMAQVVQAPDGRAYY
                     CALGSWGTAEIGADGRLHAHSLVPPDAPKWALTTSFTEALATKKGVYFSGWNGVIYWD
                     FASQKTRFFNLTAVSTIFAIADDVYASAHGQPLHRVDVEQGILGRIEGTSFRGSAVDH
                     AAVLDATHALIAARNGELFVFDGRHATRWAMQERYNLTGRVLGLQHLVDGGVAVAISG
                     KGLFFISPEGELLSALTTTHYQRITDIATREPGVLWVATE"
     gene            1330..>3314
                     /locus_tag="VEA63_10540"
     CDS             1330..>3314
                     /locus_tag="VEA63_10540"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HYD84485.1"
                     /translation="MKHQTQLGVLVLAASAVCQGLIGQTTQPPSRTAAEPEDEVVQLS
                     PFEIRAEQDTGYQATETLAGTRIRTDLKDVGAAITVLTREFIDDIGAVDNLTLLQYTP
                     NVEVGGTRSTYAGTGSAQSVSENVTNPSANTRVRGLSAADNTRDFFVSDIPWDGYNVD
                     RIDVQRGPNSILFGLGKPAGIVNASLRGADFRNRVQFQNRFGSYESLRNSLDINRVLL
                     RDELAVRLIGLVDHENFRQESAFQDDERFYGTLRWDPKFLRRNGMHTTLRIKFEDGRI
                     SSNNPRTLTPNDNVSHFFRPVAVTASNPWGGLGKGVFDPYLAATGSTVGGDNQGQLSG
                     TDPDYNPWINANSLVQQQQPVFLIDGSTGALHQAYGGLVPYAIRTPTGAIGGATNIPA
                     GFPSNSAPFLAVSAPQDFARSYQLPNYQAGLYRQWGLQDPSIFDFYNVLIDGPNKWEL
                     KTWDAFNVDLAQTAFGDRAGVNLTYDRQAYRSANEAFIGGTPSLNVDLNARFQDLSEN
                     PNAGRAFVSGAGGGGWSTTEREVARASLFGELRATDFLREGFWSKLLGRHRFNGVYAR
                     EKFEIQNRGYLLNAAAQSWYERRYTPASAATASFDDRRPTAVVYLGPSLLDYDSAAGL
                     HLPGIQAPIMMNDAPLNHFWTSWNAPSSVVFDAPWDRS"
BASE COUNT          640 a         1029 c         1010 g          635 t
ORIGIN      
        1 tcctcggtgg cgacccagag cactcccggc tcacgtgtcg cgatatccgt gatacgctga
       61 tagtgtgtcg tcgtgagcgc ggagagcagt tcgccctcgg gtgaaatgaa gaaaaggcct
      121 ttgccgctga tcgcgaccgc aacgccgcca tcgacgagat gttggagacc gagcactcgg
      181 ccggtgagat tataccgctc ctgcatggcc catcgcgtgg cgtggcgccc atcgaagacg
      241 aaaagttcgc cgtttcgcgc ggcgatcagc gcgtgcgttg catccaaaac cgcggcgtga
      301 tcgacggcgg agccgcggaa cgaggtgcct tcgatccggc cgagaatgcc ctgctcgaca
      361 tcgacgcgat gcagaggctg accatgtgcc gacgcataca cgtcgtccgc gatcgcgaag
      421 atcgtgctga ccgccgtcag gttgaaaaag cgggttttct gagaggcgaa gtcccagtag
      481 atgacgccat tccatccgct gaaatagacg cccttcttcg tggcgagggc ttcggtaaac
      541 gacgtcgtga gcgcccactt cggcgcgtcc ggcggcacga gcgaatgcgc gtgcagtctg
      601 ccgtcggctc cgatctcggc tgtgccccac gagcccaggg cgcagtagta ggcgcggcca
      661 tcgggggcct gcacgacttg cgccatgttg acccggagcg gttcgtcttt caccgcgatg
      721 tcgagccaga cggtgtcgtt gagcaccgcg taaaagcctg cgtagatcac cgcgaggcgg
      781 ccgaactgat cgaagttcaa tcgcgcaccg cgaggcatct ctccgacttc ctcgagcgga
      841 tacgagcggg tgaaggggag gccgcggacg ttgagcggtg aaagggtagc gtcgtcagcg
      901 gagccgagcg cgggcgaagc ggtggccagc gcgagcgctg cgattatcac atgacggcag
      961 cgcgaggttc gccggtggcg agcggccggg aataggccga agaaaacatc cctgcacatg
     1021 gggtggagtg gccgcagcgt gagtcgcagg cggatcgagg taaagagact ttccctggac
     1081 gacggtcgag gtgtgtggaa ttcatggata cacgacgggc gaggtccagc gtcggcgact
     1141 atggctttgg tcataacgtc catatgacca ctgcgccgtt gatggtggtg gggcgcttgg
     1201 tcgtccttcc gcagcacccg gttccccgcc gatttaccct cgcggttacc cagtccgggc
     1261 actgaccccc aactagtccc tcgagtcgtt gccgggcgtt tcgcgtccgg cggactcaac
     1321 ctcaacacca tgaagcacca aacccagtta ggcgtccttg tgctggcggc gtccgccgtc
     1381 tgccagggat tgatcggaca aaccacccag ccgcccagcc gaacggcagc ggaaccggaa
     1441 gatgaagtcg tgcagctttc gcccttcgaa attcgagctg agcaagatac cggttaccaa
     1501 gccaccgaaa cgctcgccgg cactcgcatc cgcacggatc tgaaggacgt cggagcggcg
     1561 atcaccgtcc tcacccgcga gtttatagat gacatcggcg cggtggacaa cctgaccttg
     1621 ttgcaataca ctccaaacgt ggaggtcggc ggcacgcggt cgacgtatgc gggcaccggc
     1681 agcgctcagt ccgtgagcga aaacgtcacc aatccatcgg ccaacactcg cgtccgcggc
     1741 ctaagcgcgg ccgacaacac ccgcgatttc tttgtctccg atattccatg ggatggatac
     1801 aatgttgatc gcatcgatgt gcagcgcggt cccaattcca tcctttttgg tttgggcaaa
     1861 ccggcgggta tcgtgaatgc ttccttacgc ggcgccgatt tccgcaatcg cgtgcagttt
     1921 caaaaccgct tcggttccta cgaaagcctc cgaaactcgc tcgacatcaa tcgggtgctg
     1981 cttcgtgacg agctggccgt ccgcctgatc gggctggtcg atcatgagaa ctttcgccag
     2041 gagtcggctt tccaggatga cgagcgtttt tatggcaccc ttcggtggga tcccaaattc
     2101 ctccggagaa atggcatgca caccacgctc cgcatcaagt tcgaggacgg ccgcattagc
     2161 tcgaacaatc cgcgaacgct gacgccgaac gacaatgtgt cgcatttctt tcgtccggtt
     2221 gcggtcaccg catccaaccc gtggggcggc ctgggcaaag gcgtgttcga cccttatctc
     2281 gccgcgaccg gcagcacggt cggcggagac aatcaaggcc agttgagtgg cacggatccc
     2341 gactacaatc cctggatcaa cgccaacagt ctggtgcagc agcagcagcc cgtcttcctg
     2401 atcgatggta gcaccggcgc gcttcatcag gcttatggcg gactggtccc gtatgcgatc
     2461 cgcacgccga ccggcgcgat cggcggcgcg acgaatattc ctgccggatt cccgtccaac
     2521 agcgcgcctt tcctggcggt cagtgctccg caggactttg ctcgatcgta tcaacttccc
     2581 aattatcagg cgggcctcta tcggcaatgg ggccttcaag atcccagcat cttcgatttc
     2641 tacaatgtcc tgatcgacgg tcctaacaag tgggagctga agacgtggga cgcgttcaac
     2701 gtcgacctcg cgcaaaccgc ttttggcgac cgtgcgggcg tgaatctcac atacgaccgg
     2761 caggcttacc ggagcgccaa cgaggcattc atcggcggca cgcctagcct caacgtcgat
     2821 ctcaacgcgc gcttccaaga tctttcggaa aatcccaacg ctggccgtgc gtttgtttcc
     2881 ggcgcaggcg gcggcggctg gtcgactacc gagcgagaag tcgcccgcgc ctcgcttttc
     2941 ggcgagttgc gcgcaaccga tttcctgcgc gagggcttct ggagcaagct gctgggtcgt
     3001 caccgcttca acggcgttta cgcgcgggag aaatttgaaa tccaaaatcg cggatatctt
     3061 ctcaacgccg cagctcagtc ttggtatgag cgacgctaca cgccggcatc ggccgccacc
     3121 gcgagctttg acgatcgacg gcccaccgcc gtcgtctatc tcgggccttc tctcttggac
     3181 tacgattccg ccgcagggct gcatctgcct gggatccaag cgccgatcat gatgaacgac
     3241 gcgccactga atcatttttg gacatcatgg aacgcccctt caagcgtggt gttcgacgcg
     3301 ccgtgggatc ggtc
//
LOCUS       DASPLT010001019         1127 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_277809, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001019 DASPLT010000000
VERSION     DASPLT010001019.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1127)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1127)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1127
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_277809"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..476
                     /locus_tag="VEA63_10545"
     CDS             <1..476
                     /locus_tag="VEA63_10545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD84486.1"
                     /translation="DVLLADLKLLRERELDPVLNCIHGYPRDEDPGPVRTDVFSFHAD
                     SAPVRTDTWLCTYHGAPSEGLPNEAARRRIDIPATRAALLATYGGQDDEGFYEFLRDG
                     CYDLHYAAMPQSEPYSFGVFNLWRIAVDYPGSPVPPCIHRAPATVAGEPRLLLIS"
     gene            complement(499..>1127)
                     /locus_tag="VEA63_10550"
     CDS             complement(499..>1127)
                     /locus_tag="VEA63_10550"
                     /inference="COORDINATES: protein motif:HMM:NF014722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="protein adenylyltransferase SelO family protein"
                     /protein_id="HYD84487.1"
                     /translation="SSIDRHGRYAYGNQPGVAQWNLTRFAETLLSLIDPDEKRAIEIA
                     TEALETFAHRYQHHWLAGLRRKLGLFNEEAGDATLIDALLAWMDEAKADFTNTFADLE
                     RELRPEDPRHANEGFLHWHTRWLHRLQRQPQSPAESDALRRAHNPAFIPRNHLVEAAL
                     GAAEHNDPSVMKRLLDVLATPYDHSRQAPEHRSPAPASDRCYQTFCGT"
BASE COUNT          194 a          352 c          349 g          232 t
ORIGIN      
        1 tggacgttct cctcgccgat ttgaagctcc tccgggagcg cgaactcgat ccggtgctca
       61 actgtatcca cggctacccg cgcgacgaag atcccggccc ggtgcggact gacgtttttt
      121 cctttcacgc cgacagcgca cccgtgcgca ccgacacctg gctttgcacc tatcacggag
      181 caccgagcga gggcttgccc aacgaggcag cccgccgtcg tatcgatatt ccggcgacgc
      241 gagctgctct tctcgccacc tacggcggtc aggacgatga ggggttttac gaattccttc
      301 gcgatggatg ctacgacctg cactacgccg ccatgccgca gtccgagccg tattcatttg
      361 gagtgttcaa cctctggcgc atcgccgtcg attatcccgg ttctcccgtt ccgccctgca
      421 ttcatcgtgc accggcgacc gtcgccggcg aaccgcgtct gcttctaatc agttaaaaga
      481 tccccgtcgt cacctcggct acgtcccgca gaaagtctga tagcagcgat cgcttgcggg
      541 tgcaggcgaa cgatgctccg gagcctggcg tgagtgatcg taaggcgtcg caagaacgtc
      601 gagcagccgc ttcatcacgc tggggtcgtt gtgctccgcg gcgccgagag cggcttcgac
      661 gagatgattg cgcgggatga atgcgggatt gtgcgcgcga cgaagcgcgt cggattcggc
      721 gggcgattgc ggctgacgct gcaagcggtg aagccaacgc gtgtgccagt ggagaaatcc
      781 ttcgttcgcg tggcgcgggt cctcgggacg aagctcgcgt tcgagatcgg cgaaggtgtt
      841 cgtgaagtcc gccttcgctt cgtccatcca cgcgagcagc gcatcgatca acgtggcatc
      901 gccagcctct tcattgaaca aaccgagctt ccgtcggagc ccggcgagcc aatggtgctg
      961 atagcggtgc gcgaaggttt cgagtgcttc ggtcgctatc tcgatcgcac gtttttcatc
     1021 tggatcgatc agtgacagca gcgtctccgc gaagcgcgtg aggttccact gcgcgacgcc
     1081 cggctggttc ccgtaagcgt agcggccgtg gcgatcgatt gagctaa
//
LOCUS       DASPLT010001020         2213 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_135545, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001020 DASPLT010000000
VERSION     DASPLT010001020.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 2213)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2213)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2213
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_135545"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2133)
                     /locus_tag="VEA63_10555"
     CDS             complement(<1..2133)
                     /locus_tag="VEA63_10555"
                     /inference="COORDINATES: protein motif:HMM:NF025965.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4450 domain-containing protein"
                     /protein_id="HYD84488.1"
                     /translation="MRTAEGTKWLHDAARITTRYRPGELLYDIHDPLLGAQTLHVAAV
                     AHFETEGTIVRVQLVAADARRDANTNVPAVELVWAYGGVNGQRGKRDGDIGTENVPIS
                     QWFQLQPEFCRGNQFTLAPEGFRLHADNIAIAGVTPANASLTLADATHWNDLPTLLAS
                     PAGLRPEMPVVVGRAALTSGEPLLLSLQRVAIDAASSVAADDLDTYRAVTAGNSAAPP
                     RAASPQLASPWSAAELPHAFRAAQRHFRELRTRVTVNTPDPFLNAAVGALNVAADAAW
                     DAPQQAIMHGAIAWRTRLLGWRGPYALDALGWHDRARAHFTYWSGRQNLSPIPEKLPP
                     ADENANLARNETGLHSNGDLSNSHYDMNLVAIDALFRHLQWTGDLEFAAKLWPMIERH
                     LAWERRLFRREFGAEKLPLYEAYAAIWASDDLQYHGGGTAHASAYNYYHNTQAARLAR
                     LLGHDAAPYEREAELIARGLRAHLWLPERGGFAEFKDLLGLQRVHPSAGVWTFYHLMD
                     SGFPTAQEAWSMARRVDTEIPHLPVRGPGVPDNLYVVATTNWMPYTWSINNVVMSEIA
                     HTALGFWQAGRPEDAWKMTKGALTAAMFMGISPGNVGSMSYLDVYRRESQRDFADGSG
                     VLSRALIEGLFGLTPDALAGELHVTPGFPAAWNHAQLRHPDLSLRFTREGDLDHYVIT
                     QRFTQPQALRFKVLAHGTRVASVAVNGDA"
BASE COUNT          387 a          654 c          793 g          379 t
ORIGIN      
        1 cgcgtcgccg ttgaccgcga ccgatgcgac gcgagtgcca tgggcaagaa ccttgaatcg
       61 cagcgcctgc ggttgggtga aacgttgcgt gatcacgtag tggtcgaggt cgccttcgcg
      121 cgtgaagcgc agcgacagat cgggatggcg cagttgcgcg tggttccaag ctgcggggaa
      181 tccaggagtg acatgaagtt cgccggcgag cgcgtccggg gtgagaccga agagtccttc
      241 gatcaatgct cgcgagagca cgccggaacc gtccgcgaaa tcgcgctgcg attcccgacg
      301 gtaaacatcg agatacgaca tcgagccaac attgcctggc gagatgccca tgaacatcgc
      361 ggccgtgagc gcgcctttcg tcattttcca agcgtcctcg gggcgaccgg cctgccagaa
      421 tccgagtgcg gtgtgcgcga tctcggacat cacgacgttg ttgatcgacc acgtgtaagg
      481 catccagttg gtcgtcgcga cgacgtagag attgtcaggc acaccgggac cgcgcacggg
      541 cagatgcgga atttcggtgt cgacgcgccg cgccatcgac cacgcctctt gcgcggtggg
      601 aaatccggaa tccatgagat gataaaacgt ccagacgccg gcgcttgggt gcacgcgttg
      661 caggccgagc agatccttga attcggcaaa gccgccgcgt tcaggcagcc agaggtgagc
      721 gcgcaggccg cgggcgatca gctccgcctc gcgctcgtag ggcgccgcat cgtggccgag
      781 caggcgggcg agccgcgcgg cctgcgtgtt gtgataataa ttataggcgg atgcgtgcgc
      841 ggtgccgccg ccgtgatatt gcaggtcgtc gctcgcccag atcgcggcgt aggcttcgta
      901 gagcggaagt ttttcggcgc caaactcacg gcggaacaaa cgccgctccc acgcgagatg
      961 ccgctcgatc atcggccaca gcttcgcggc gaactcgagg tcgcccgtcc actgcaggtg
     1021 gcggaacaac gcgtcgatcg cgacgaggtt catgtcgtag tgcgaattcg agagatcgcc
     1081 gttgctgtgg aggccggttt cgttgcgcgc gaggttcgcg ttctcgtcgg ccggcggaag
     1141 tttttccgga atcggtgaaa gattttggcg gccgctccaa tacgtgaaat gcgcgcgagc
     1201 acgatcgtgc cagcccaacg catcgagcgc gtagggaccg cgccagccga gcagccgcgt
     1261 gcgccacgca atcgcgccgt gcatgatcgc ctgctgtggc gcgtcccagg cggcgtcggc
     1321 ggcgacattg agcgcgccga cggcggcgtt gagaaaggga tccggcgtgt tcaccgtcac
     1381 gcgcgtgcga agctcgcgaa agtgacgttg ggcggcgcga aacgcgtgcg gcagttcggc
     1441 cgcggaccat ggtgatgcga gttgcggcga ggcggcgcga ggtggagcgg cggagttgcc
     1501 ggcggtcacg gcgcgatagg tgtcgagatc gtcggcggcg acggaactcg cggcgtcgat
     1561 cgcgacgcgt tgcagggaaa gcagcaacgg ttcgccggag gtcagcgccg cgcgaccgac
     1621 gaccacgggc atttctggac ggagcccagc cggcgacgcg agcaaggttg ggagatcgtt
     1681 ccagtgcgtg gcgtcggcga gcgtgagcga tgcgtttgcc ggagtgacgc cggcgatggc
     1741 gatgttgtcg gcgtgcaggc ggaaaccttc gggcgcgagc gtgaattggt tgccgcgaca
     1801 gaactccggc tggagttgaa accactggct gatcggcacg ttttctgtgc cgatgtcgcc
     1861 gtcgcgtttt ccacgctggc cgttcacgcc gccgtaggcc cagacgagct cgacggcggg
     1921 cacgttggtg tttgcgtcgc ggcgtgcgtc tgccgcgacg agttggacgc gcacgatcgt
     1981 tccttctgtt tcgaaatgcg ccacggcggc cacgtgcaac gtctgcgcgc cgagcagcgg
     2041 atcgtggata tcgtagagca gctcgccggg ccgataacga gtagtgatgc gcgcggcgtc
     2101 gtgcagccat ttggttccct cggcggtgcg caagccgagc cgcagatttc caccgcgacc
     2161 tggcagataa agaacgaatt cgggacggtc gccgccatcg gtgcgaaacg ccg
//
LOCUS       DASPLT010001021         1203 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_176589, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001021 DASPLT010000000
VERSION     DASPLT010001021.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1203)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1203)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1203
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_176589"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..974)
                     /locus_tag="VEA63_10560"
     CDS             complement(<1..974)
                     /locus_tag="VEA63_10560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated protein Cas3"
                     /protein_id="HYD84489.1"
                     /translation="MTATPPSEAREEQKITLSPADHDHPVLKSRLKHAKPAQLIVEVK
                     AKGKLRDEQMAKRLADEAETILAKNPPRSIAIMVNRVATARSVAEKMEKANKGRVSLL
                     IGRLRPIDREAVTKGIQHWLKTGATASSAEHAPLIVVSTQCLEVGADLDFDALVTEAA
                     SLDALRQRFGRLNRGGRDIVARAVIVLPGDQDLSLDKLDDAAPCDPIYGNAIPRAWEW
                     LRSIAKDDIVDFGINAMAAAVDQLRSKDPEALTAMLSPTKNAPVLLPAYLDCWVQTNP
                     APAVEPDVALFLHGPQRDMTEVQVCWRGDLPATANKEEWADILSLCPPT"
     gene            1023..>1203
                     /locus_tag="VEA63_10565"
     CDS             1023..>1203
                     /locus_tag="VEA63_10565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD84490.1"
                     /translation="MPTVFLHPLQRLDKRPTDMRLIDDQEAVVRDESRVDRASLRTHA
                     ISAEKQTRTGLIHGAA"
BASE COUNT          220 a          349 c          370 g          264 t
ORIGIN      
        1 gtgggagggc agaggctcag aatatcggcc cattcctcct tatttgccgt agccggtaag
       61 tcaccgcgcc agcagacttg cacttcggtc atgtcgcgct ggggaccatg gagaaagagc
      121 gcgacatcgg gttcgaccgc tggtgcggga ttcgtctgaa cccaacaatc gaggtatgcg
      181 ggtaacagca ccggggcgtt ctttgttggc gaaagcatcg ctgtgagtgc ctccgggtct
      241 ttgctgcgaa gttggtcgac cgccgcggcc atggcgttga tgccgaaatc gacgatatcg
      301 tctttcgcga tgctccttag ccactcccac gcccgcggga tcgcgttgcc gtaaatcgga
      361 tcgcaagggg cggcgtcatc gagcttgtcc aacgaaagat cctggtcacc gggcaagaca
      421 atcacggcgc gagcgacgat gtctctacca cctcgattga ggcggccgaa acgttgacgc
      481 agagcgtcga ggctggctgc ctccgtgacg agcgcgtcga aatccaagtc ggctccaact
      541 tccaagcact gggttgaaac gacaatgagc ggagcgtgtt cggcggaact cgccgttgct
      601 cctgttttga gccagtgttg aattcctttg gtgactgctt cccggtcgat cggacgcaag
      661 cgtccgatca gaagcgatac gcggcccttg ttcgctttct ccattttttc cgccacggag
      721 cgggcggtcg ccactcggtt gaccataatg gcgatcgagc gcggaggatt tttggcgagg
      781 atcgtttccg cttcatcggc gaggcgcttc gccatttgct cgtcgcgaag tttgcctttc
      841 gccttcacct cgacgatgag ctgcgctggt ttcgcgtgct tcagtcgcga tttcaagacc
      901 gggtggtcgt gatccgctgg actgagggtg atcttctgtt cctcccgcgc ttcgctcgga
      961 ggcgtagcgg tcatctgtat aaactcaaaa ggaagcctca acgtttccgc gccgtcggtc
     1021 gcgtgccgac ggtatttctc cacccactgc agcgtctgga taaacggccg actgatatgc
     1081 gcctcatcga tgatcaagag gctgtcgtgc gcgacgagag ccgcgtggat cgggcgagct
     1141 tgcggactca cgccatatcc gcggaaaagc agacgcgaac cggcctgatc cacggtgctg
     1201 caa
//
LOCUS       DASPLT010001022         1003 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_72324, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001022 DASPLT010000000
VERSION     DASPLT010001022.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1003)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1003)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1003
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_72324"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..341
                     /locus_tag="VEA63_10570"
     CDS             <1..341
                     /locus_tag="VEA63_10570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD84491.1"
                     /translation="LTRTFSFFRKEQRIEIVDEVEFSEASAFETAFVTNSTWQEHGGG
                     AFTVSDGGTALDVVVKSETGELDLKTQPVTAPRQKPGIAPTRLGYDLREPARFARIKA
                     TITVRAPGGQ"
     gene            637..>1003
                     /locus_tag="VEA63_10575"
     CDS             637..>1003
                     /locus_tag="VEA63_10575"
                     /inference="COORDINATES: protein motif:HMM:NF015735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HYD84492.1"
                     /translation="MKFICLGYINHNAFASVPEAEQLAMIDSCCAYDDELRANGHIKG
                     GEGLQPPNTAMTLRYANGKVVVTDGPFAETKEQLGGIMILEARDLNHAVELISKHPGA
                     KMGPWEIRPAADLDEMVRQS"
BASE COUNT          218 a          295 c          307 g          183 t
ORIGIN      
        1 aactgacgcg gacattctcg tttttccgaa aagagcagcg catcgagatc gtcgacgaag
       61 tggagttctc ggaagcgagc gccttcgaaa ccgcattcgt caccaactcc acgtggcaag
      121 aacacggtgg aggtgcgttc actgtttccg atggcgggac tgcgctcgac gtcgttgtga
      181 aatccgaaac gggagagctg gacttgaaga cgcagccggt gaccgcgccg cggcagaagc
      241 cgggaatcgc gccgacgcgg ctcggctacg atctccgcga gcctgcgcgt ttcgcacgga
      301 tcaaagcgac gatcacggtg cgcgcgccgg gcgggcagtg atgccgttcg aaggctcgcg
      361 cggctgagcg gcgaattcag gcaaaaaaaa tttcgctcgc tgtcgaaatc ctccacgttc
      421 gttcggctag ttgatgaacg ggctcgattc cgggtcctca ctcctcacca cgaacaacgc
      481 aaatcgagtt gaaccacaaa ggcacgaaga cacggagaag atgcgaccaa ggtaacgagc
      541 ggatcgcgcg gcggccctgc gtgatgctcg ccgccaggtt ttggcttttc ctctgtgtct
      601 ccgtgctcgg tggttcaact cagctctgac ctcaccatga aattcatctg tctcggttac
      661 attaatcaca atgctttcgc ttccgtgccg gaggccgaac aactcgcaat gatcgacagt
      721 tgctgcgcct acgacgacga gctccgcgcg aacgggcaca tcaaaggcgg tgaagggctg
      781 cagccgccga acaccgcgat gacgctgcgt tacgcgaacg ggaaggtcgt cgtcacggac
      841 gggccttttg ctgaaaccaa ggagcagctc ggcggcatca tgattctcga agcgcgcgac
      901 ttgaaccacg ctgtcgagct gatctcgaag catcccggcg cgaagatggg cccttgggaa
      961 atccgtccgg cggccgacct ggatgaaatg gtccgacaga gcg
//
LOCUS       DASPLT010001023         5263 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_12807, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001023 DASPLT010000000
VERSION     DASPLT010001023.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 5263)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5263)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5263
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_12807"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..597
                     /locus_tag="VEA63_10580"
     CDS             <1..597
                     /locus_tag="VEA63_10580"
                     /inference="COORDINATES: protein motif:HMM:NF020952.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HYD84493.1"
                     /translation="EAAVQRLAQMARAAGIHVIMATQRPSVDVITGTIKANFPTRISY
                     QVTSKIDSRTILGEQGAEQLLGQGDLLFMAGGGRIRRLHGPFVTDAEVERVVSMLKEQ
                     GAPQYRTDVTAEPNENGADDNPELFADGEGGDDLFDRAVEIVQRDRKASTSYLQRRLS
                     IGYNRAATLIEKMEKAGIISEANAAGKRDILVDVVDED"
     gene            664..2259
                     /locus_tag="VEA63_10585"
     CDS             664..2259
                     /locus_tag="VEA63_10585"
                     /inference="COORDINATES: protein motif:HMM:NF015210.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HYD84494.1"
                     /translation="MAKITTSRIATEVELISALIAGINKGEIKVPKFQRSFVWKDEQA
                     LNLLDSIANNYPVGSLLLWRTKEKLLAERNIGEFSLPATDDMEPTDYVLDGQQRLTVI
                     YSCLGAKESEGGFAVVYDLETQKFIRLPDTLKLTHFPLRKLFITTQLLNFRAAIQVAP
                     NGSLYQERLDALIGAFSDYRLPVVTLKDLSVEEVCPIFERINSSGTRLSTYDLMVAAT
                     WNKSFDLNDEVDEIRDALEPKGFGDIDRETILKCMSAVQLGTIKEQSLMTLRELNKDQ
                     MRALITTTKAALLSTVDLLSTEFKIYSWDFLSYEALAVVICHLYAKDNHLASDQVRRV
                     RQWFWRASFGERYKVGGEGFVSNDLKLVRAFVIDGTGAADDFGKPPTAEEWLSIAFRS
                     NVSRTRAFVLALASLSPRNLTNGAVIDVTQALSSYNKKQFHHIYPRAHLRRLRTETND
                     NLVANICMLAAAANNAVSDNDPATYLPHYATALAEAADQVFASNAMPLPSNFDYSKAT
                     YDEFLKERSTIIASLAEALCNGS"
     gene            complement(2278..2808)
                     /locus_tag="VEA63_10590"
     CDS             complement(2278..2808)
                     /locus_tag="VEA63_10590"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HYD84495.1"
                     /translation="MTKPSKTKQLGKLSKPAVLKDGHDVAAFACGREQIDAWLKQRAK
                     KAIESDTARTFVVCRGAKRVVGYYALAAGAVERAGVSGALSRNTPDPIPVIILARLGV
                     DQSEKGRGLGRDLLADAMRRALQAAKIIGARALVVHALDADAARFYEAHGFARLRAEE
                     EGTLFVAMKTLRDGLG"
     gene            complement(2891..3211)
                     /locus_tag="VEA63_10595"
     CDS             complement(2891..3211)
                     /locus_tag="VEA63_10595"
                     /inference="COORDINATES: protein motif:HMM:NF020265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HYD84496.1"
                     /translation="MAVKAKRKRAQAERIAPVKTRAAPMSFRIDEATRDLVDRAATAS
                     GQNRTDFMLTVLREKASEVLLNQRLFTLNGADWDAFAERLDDPPPPNAKLKALLARTP
                     LWDR"
     gene            3990..>5263
                     /locus_tag="VEA63_10600"
     CDS             3990..>5263
                     /locus_tag="VEA63_10600"
                     /inference="COORDINATES: protein motif:HMM:NF015211.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase family protein"
                     /protein_id="HYD84497.1"
                     /translation="MKQRTWHAHLAHNEHNLAYIAARFAVPLHTARSAVKTLRTTPNP
                     QSAAAFWRNIAHAAQLPPQAPWATWLFQGGRGAGKTRAGAEWLAERAEQSPNGVFALV
                     GATHRDVREVMIDGVSGLRALTHRPPPRYEASRQRLVWPNGAIAYCFSAQEPERLRGP
                     QFDAAWADEFCVWPKPADTLSNLRLALRRGPNPQLVVTTTPKPLQSLKHLRAEPGCIT
                     TLAPTTENAAHLSPAFIAHVRALYGGTRLEAQELEGLIVENESALFSAELFMRARCTP
                     PARFDRIVVAVDPPAGVTGSACGIVVAGCVQASDTHSFTPAQDARAPALDECVSLAET
                     PHAYVLEDATAHGLSPLQWATRAVDAARRHNAHAIVAEANQGGEMVRTVLAQANPPCP
                     VILVHASEGKRARAQPIALLYEQGRIRHCGGLA"
BASE COUNT         1076 a         1794 c         1464 g          929 t
ORIGIN      
        1 gaagctgccg tgcagcgtct cgcgcaaatg gcgcgcgccg ccggcatcca cgtcatcatg
       61 gcgacgcagc gccccagcgt cgacgtcatc accggcacga tcaaagccaa cttcccgacc
      121 cgcatctcct accaggtcac ctcgaagatc gactcgcgca ccatcctcgg cgaacaaggc
      181 gccgaacaat tgctcggcca aggcgactta ttgttcatgg ccggcggcgg ccgcatccgg
      241 cgcctgcatg gcccgttcgt caccgacgcc gaagtcgaac gcgtcgtctc gatgctgaaa
      301 gagcaaggcg cgccgcaata tcgcaccgac gtcaccgccg agccgaacga aaacggcgcc
      361 gacgacaatc ccgaactctt cgccgacggc gaaggcggcg acgacctctt cgaccgcgcc
      421 gtcgaaatcg tccaacgcga ccgcaaagcc agcaccagct atctccagcg ccgcctctca
      481 atcggctaca accgcgccgc cacgctgatc gagaaaatgg aaaaggcggg gatcatctcg
      541 gaggcaaacg cggccgggaa acgggatatc ctggtcgatg tggtggatga ggattgagtg
      601 acccacctgc ttcaggctgc tcgcgactcg acacatggtc tatgtccaga ctgagcgaag
      661 gcaatggcca agataactac cagtaggatc gcgaccgagg tcgagcttat cagcgcccta
      721 atcgctggta tcaacaaggg cgaaatcaaa gtcccaaagt ttcagcgctc gtttgtttgg
      781 aaggacgagc aggcactgaa tcttctcgac agcatcgcga acaactaccc cgtgggtagc
      841 ctcctgttat ggcgaacaaa ggaaaagctg ctcgcagaaa ggaatatcgg cgagtttagc
      901 ctccctgcaa ctgacgatat ggagccgacc gactacgtac tcgacggcca gcaacggctc
      961 acggttattt attcgtgcct cggagcgaaa gaaagtgaag gcggatttgc cgtcgtttac
     1021 gatcttgaga cgcagaagtt catccgcttg ccggatacgt tgaagctgac gcacttccca
     1081 cttcgcaagc tatttatcac aacgcaactc ctgaactttc gtgcggcaat ccaagtagct
     1141 ccaaacggat cattatatca ggaacgcctc gacgcgctga tcggtgcgtt ctcagactat
     1201 cgcctgccag tggtgacgct caaagatctc tctgtggaag aggtttgccc aatattcgaa
     1261 aggatcaata gttctgggac gaggctgtcg acatatgacc tgatggtcgc agctacttgg
     1321 aacaagtctt ttgatctcaa cgacgaagtc gacgagatac gtgacgcctt ggagccaaaa
     1381 ggctttggtg acattgatcg agaaacgatc ctcaagtgca tgtccgcggt tcagctcgga
     1441 acgatcaaag agcagtcttt gatgacgctt cgagagctaa acaaggatca aatgcgggcg
     1501 ctaattacga caacaaaagc ggccttgctg agcaccgttg accttctttc gactgaattc
     1561 aaaatctaca gctgggactt cctctcgtat gaagctctgg cggttgtcat ctgccatctg
     1621 tacgcgaagg acaaccacct cgcttcggat caggtgcgtc gcgtccgtca gtggttttgg
     1681 cgcgcctcct tcggggagcg ctataaagtc ggcggagagg ggtttgtatc gaacgatctg
     1741 aagctagtac gcgcgttcgt tattgatggc accggagcgg cggatgattt cggcaaacca
     1801 ccaacggctg aagagtggtt gtccatcgcg tttaggagca atgtgtccag gacacgagct
     1861 tttgttctag ctctggcaag cctaagccct cgcaacctta caaacggtgc agtcattgat
     1921 gtgacgcaag cactgtcatc gtacaacaag aagcaatttc accacatcta tccccgcgcg
     1981 catctcagaa gactcaggac agagacgaac gacaatctgg tggcgaacat ctgcatgctt
     2041 gccgccgccg caaacaacgc cgttagcgat aacgatccgg ccacgtatct cccgcattat
     2101 gctacggcgc tggccgaagc ggcagatcaa gtcttcgcct ccaatgcgat gccgcttcct
     2161 tcgaacttcg actactctaa agcaacgtac gacgagtttc tgaaagagcg ctcgacaatc
     2221 attgcatcgc tggcagaggc cctgtgcaac ggcagctaga cgatcgccgg ctgacactca
     2281 ccccaaccca tcccgcaacg tcttcatcgc cacaaacagc gtcccctctt cctccgcccg
     2341 cagccgcgca aatccgtgcg cttcgtaaaa ccgcgccgcg tccgcatcca acgcatgcac
     2401 caccagcgcc cgcgcgccga tgatcttcgc cgcctgcagc gcgcgtcgca tcgcgtcggc
     2461 caacagatcg cgccctaaac cccgcccctt ctcgctctga tcgacaccga gccgcgccag
     2521 gatgatcacc gggatcggat caggcgtgtt gcggctcaac gctccgctca cgcccgcgcg
     2581 ctccaccgcg ccggccgcaa gcgcgtaata gccgacgacg cgcttggcgc cgcggcaaac
     2641 aacgaacgtg cgcgccgtgt cgctctcgat cgccttcttc gcccgctgct tcagccaagc
     2701 atcgatctgc tcgcggccgc aagcgaacgc ggccacgtcg tgaccatcct tcagcacggc
     2761 gggcttgctc agcttgccga gctgcttggt ctttgaaggc ttcgtcattc cggacgcgcg
     2821 gcttgtccgc cgaagctcca agagcgaagg cggaagcgcg atgccccgga aggacgagtt
     2881 gaaccgttca ctaacgatcc cagagcggcg tccgcgccag cagcgctttg agcttggcgt
     2941 tcggcggcgg cggatcgtcc agccgctcag cgaacgcgtc ccagtccgcc ccgttcagcg
     3001 tgaagagccg ctggttcagc agcacttcgc tcgccttctc ccgcagcacc gtcagcatga
     3061 aatcagtccg gttctgcccg gacgccgtcg ccgcgcggtc caccagatcg cgcgtcgcct
     3121 cgtcgatacg gaaactcatc ggcgcggctc tggttttgac cggggcgatc cgctctgcct
     3181 gcgcccgctt ccgcttggct ttgactgcca tgggccttct cctccgggcg actgtacgcg
     3241 gccattcttg ctggtgtatt acactgtcaa tacatatcgc ccgtgtcaat acatccacgc
     3301 gccgccccca aaccttacca aaatttcgta tcccccccct tccagccagc cgtacactcc
     3361 tcctcgtctc cacacgggag ggtttcgaag agctccgaac gctttggaat gtggaaacat
     3421 cggccggcgc ggcaggcgtc gcttcgatga aagggcgaaa gctccgacat cgttgagaaa
     3481 gtccggtgat ggcgaaggcc ttcaacatca tcaggcgaag cgaacgacgc ttgcattgct
     3541 cgcggggcat gaaaccgcga ggtccagcat tcttcggaag atgctgacgc gctgcaccgg
     3601 ctcgtggtca taaggagcta agaccgctgc gtgcgaaacc atgcagctga gtcaaaaact
     3661 cctggctcgc caatccggga ataacggccg aatacggggc gcgttcgacg cgcgtgttgt
     3721 gcgggcgatc gcgcagcaca ttttgagact acctcggcgc gtccgacgcg ccccgacctc
     3781 ctgctttcgc gggagccacg attttcgatc cgcaagatca ccaaggatgg cgcgcgcctg
     3841 aaaccgcgca cccctgcgcg tccatttgtt ctccgtcatc ccggacgcgc ggagcgcgat
     3901 ccgggaccca gaacaaacgc acagcttgcg gcccctgggt cccgggtctc gcttcgctcg
     3961 cccgggatga cggtgttgag gagaaacgca tgaaacaacg cacctggcac gcacatctcg
     4021 cccacaacga acacaacctc gcttacatcg ccgcccgttt cgccgtgccg ctgcacaccg
     4081 cgcgcagcgc cgtcaaaaca ctgcgcacca cgccaaaccc acaatccgcc gccgcgttct
     4141 ggcgcaacat cgcccacgcc gcacaactgc cgccacaagc gccctgggcc acctggctct
     4201 tccaaggcgg ccgcggcgcc ggcaagacgc gcgccggcgc cgaatggctg gccgagcgcg
     4261 cagagcaatc accaaacggc gtcttcgccc tcgtcggcgc cacgcaccgc gacgtgcgcg
     4321 aagtgatgat cgacggcgtc tccggcttgc gcgcgctcac gcaccggccg ccgccgcgtt
     4381 acgaagcctc gcgccagcgc ctggtctggc ccaatggcgc gatcgcctat tgcttcagcg
     4441 cccaagagcc ggagcgtttg cgcgggccgc aattcgacgc cgcctgggcc gatgaattct
     4501 gcgtctggcc caagcccgcc gacacgctct cgaacctgcg gctcgcgctc cgccgcggcc
     4561 ccaatccgca actcgtcgtc accaccacgc cgaagccgct gcaaagcttg aagcacctgc
     4621 gcgccgaacc cggctgcatc accacgctcg cgccgacgac ggaaaacgcc gcgcatctct
     4681 cgcccgcctt cattgcccac gtccgcgcgc tttacggcgg cacgcgcctt gaagcgcaag
     4741 aactcgaagg cctgatcgtc gaaaacgaat ccgccctctt cagcgccgaa ctcttcatgc
     4801 gcgcacgctg cacgccgccc gcccgcttcg accgcatcgt cgtcgccgtc gatccgcccg
     4861 caggcgtcac gggttcggcg tgtgggattg tcgtggcggg ctgcgtacag gcgagtgaca
     4921 cacactcgtt cacgcccgcg caagacgctc gggcgccagc gctcgacgag tgtgtgtcac
     4981 tcgctgagac accgcacgct tacgttctcg aagacgccac cgcgcacggc ctctcgcccc
     5041 tgcaatgggc gacgcgcgcc gtcgacgccg cccgccgcca caacgcgcac gccatcgtcg
     5101 ccgaagccaa tcagggcggc gaaatggtgc gcaccgtgct ggcgcaagcg aacccgccgt
     5161 gccccgtcat tttggtgcac gccagcgaag gcaaacgcgc ccgcgcccag ccgatcgcgc
     5221 tcttgtacga acaaggccgc atccgccatt gcggcggctt ggc
//
LOCUS       DASPLT010001024         1388 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_216228, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001024 DASPLT010000000
VERSION     DASPLT010001024.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1388)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1388)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1388
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_216228"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..316
                     /locus_tag="VEA63_10605"
     CDS             <1..316
                     /locus_tag="VEA63_10605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012377107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent DNA ligase"
                     /protein_id="HYD84498.1"
                     /translation="GDELKFAGKVGTGFNTKLLGTLHAKFRKIAVDTCPFADLPEKRR
                     ARYGQGMTPAEMRRCHWVKPVLVAQIKFAEWTRDDRLRQPVFLGLREDKAAREVARER
                     TQ"
     gene            320..1237
                     /gene="ligD"
                     /locus_tag="VEA63_10610"
     CDS             320..1237
                     /gene="ligD"
                     /locus_tag="VEA63_10610"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012377106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-homologous end-joining DNA ligase"
                     /protein_id="HYD84499.1"
                     /translation="MATKTEMMVGNTRLMVSNLDKVLYPKVGFTKGNVIDYYVRVSRD
                     LLPHLKDRPITLKRYPDGVDGFFFYEKQCPSHRPKWVKTAKVPRDRGGEINYCVMNDL
                     PALVWAANLADLELHTFLHKAPAIERPTAIAFDLDPGPPADIVLCCQVGLWVKALFDG
                     LGLKSFAKTSGSKGLQVYVPLNTPVTYEATKAFAHTVAQVLERQFPKVVVEKMLKSLR
                     KGKVLVDWSQNDDHKTTVNVYSLRAKERPTVSTPVTWDEVEATWKKKKKELLTFETED
                     VLKRIEKHGDLFAPVLQLKQKLPPIKVLG"
BASE COUNT          289 a          345 c          505 g          249 t
ORIGIN      
        1 cggcgacgag ctgaagtttg cgggcaaggt cggcacgggt ttcaacacga agctgctggg
       61 cacgctgcat gcgaagtttc gtaagatcgc cgtggatacg tgtccgtttg ccgatctgcc
      121 ggagaagcgc cgggcgcgtt acgggcaggg gatgacgcct gcggagatgc ggcggtgcca
      181 ctgggtgaag ccggtgctgg tggcgcagat caaattcgcc gagtggacgc gcgacgaccg
      241 gctgcgtcag ccggtgtttc tcggactgcg cgaggacaag gcggcgcgcg aagtcgcgcg
      301 cgaaagaacg caataaatca tggccacgaa aaccgaaatg atggtgggca acacgcgcct
      361 gatggtgagc aatctcgaca aggtgctcta cccgaaggtg ggtttcacga agggcaacgt
      421 gatcgattac tacgtgcggg tgtcgcgcga tctgctgccg catttgaagg accggccgat
      481 tacgttgaaa cgttatccgg atggcgtgga cggatttttt ttctacgaga agcagtgtcc
      541 gtcgcaccgg ccgaagtggg tgaagaccgc gaaggtgccg cgggatcgcg gcggcgagat
      601 caattattgt gtgatgaacg atctgccggc gctcgtgtgg gcggcgaatc tcgccgacct
      661 ggagttgcac acgttcttgc acaaagcgcc ggcgatcgag cggcccacgg cgatcgcgtt
      721 cgacctcgat ccgggcccac cggcggacat cgtgttgtgc tgccaggtcg ggctgtgggt
      781 gaaagcgttg ttcgacgggc tcggcttgaa gagcttcgcg aaaacgtcgg gctcgaaagg
      841 gctgcaagtt tacgtgccgc tcaacacgcc ggtgacttac gaggcgacga aggcgtttgc
      901 gcatacggtg gcgcaggtgc tcgagcggca gtttccaaag gtggtcgtcg agaaaatgct
      961 gaagtcgctg cgcaagggaa aggtgctcgt cgactggagt cagaacgacg atcacaagac
     1021 gacggtgaac gtttactcgc tgcgcgcgaa ggaacggccg acggtgtcga cgcccgtgac
     1081 gtgggacgag gtcgaagcga cgtggaagaa aaagaagaaa gagctgctga cgttcgaaac
     1141 ggaggacgtg ttgaagcgaa tcgagaagca cggcgatctt ttcgcgccgg tgttgcagtt
     1201 gaagcagaag ctgccgccga tcaaggtgct cgggtgagtc gcgccgggtc gtcgtgaaaa
     1261 ccgcgcggtt cgcaaacgtg gtgaaggaag cgggcgcgcc ggaggcatat ttgttgtggg
     1321 cgtcgccgaa gaaggatcgg gtgtttcagc gcgcggtgaa ggagcaacgc gtgttgacgg
     1381 tgcaccag
//
LOCUS       DASPLT010001025         1086 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_236268, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001025 DASPLT010000000
VERSION     DASPLT010001025.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1086)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1086)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1086
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_236268"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..821
                     /locus_tag="VEA63_10615"
     CDS             <1..821
                     /locus_tag="VEA63_10615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007363232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acetylxylan esterase"
                     /protein_id="HYD84500.1"
                     /translation="YQTLGIGGGPVRFYGMLTEPTKPGRYPAVLRVPGAGVRSYRGDV
                     ALAASGLIVFEIGIHGIPVNLPDAVYEDLRGGALRGYQAYNLDDPLQYYYLRVYLGCV
                     RANDYLVTHEKWDGKNLVVAGGSQGGQLSIVTSALDPRVTGTVANYPAYCDVTGYLHG
                     RAGGWPHTLANAENQTEAKVRTTRYYDAVNFARRLRAPASFAWGYNDETCPATSMHAA
                     YNVITSPKELWLQLNMGHPSIPEFSETFRQRTLAMANNPATALAPNAGSAVGTR"
     gene            complement(903..>1086)
                     /locus_tag="VEA63_10620"
     CDS             complement(903..>1086)
                     /locus_tag="VEA63_10620"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009507755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HYD84501.1"
                     /translation="GSDLSHVVQTRNYVRDDANLTRYNELYREYFSAPFPARTTITNC
                     LPVALHYEIECIAVVK"
BASE COUNT          204 a          359 c          319 g          204 t
ORIGIN      
        1 gttatcaaac gctcgggatc ggcggcggcc cggtgcgttt ctacggcatg ttgaccgaac
       61 ccaccaagcc cggccgctat cccgccgtgc tgcgcgtgcc cggtgccggt gtgcgttcat
      121 atcgcggcga cgtcgcgctc gcggcgagcg gattgattgt attcgaaatt gggattcacg
      181 gcatcccggt gaatctgccc gatgccgttt acgaggacct gcgtggcggt gcgcttcgcg
      241 gctaccaagc ttacaatctc gatgatccgc tccagtatta ttacctgcgc gtgtatctcg
      301 gctgcgtgcg cgccaacgat tatctcgtca cgcacgagaa gtgggatggc aaaaacctcg
      361 tcgtcgccgg tgggagccag ggcggacagc tttcgatcgt cacctcggcc ctcgatccgc
      421 gcgtcaccgg caccgtcgcc aactaccctg cgtattgtga cgtcaccggt tacctccacg
      481 gccgcgccgg cggctggccg cacacgctcg ccaacgccga gaaccagact gaggcgaagg
      541 ttcgcacgac cagatattac gatgcggtca acttcgcgcg gcggctgcgc gcgccggcaa
      601 gtttcgcgtg gggttacaac gacgagacct gccccgccac cagcatgcac gccgcctaca
      661 acgtcatcac ctcgccaaag gaactctggc tccagttgaa catgggccat ccatcgattc
      721 ccgagttttc ggaaaccttc cggcaacgca cgcttgcgat ggcgaacaac ccggccacag
      781 ctctcgcgcc aaacgccgga agcgccgtcg gcacgcgctg aacgcgttca tcagcgaaaa
      841 atcgtcacgc ctgttcgccg gcgtgacgat ctcgaggccc ggcagcagtg atcgtccaat
      901 cgctatttca cgacggcgat gcactcgatc tcgtaatgca acgcgaccgg cagacagttc
      961 gtgatcgtgg tccgcgccgg aaacggcgcc gaaaaatact cgcggtaaag ttcgttgtag
     1021 cgcgtcaggt tcgcgtcatc gcgcacatag ttgcgcgttt gaacgacgtg actcaggtcg
     1081 ctgccc
//
LOCUS       DASPLT010001026         2909 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_51705, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001026 DASPLT010000000
VERSION     DASPLT010001026.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 2909)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2909)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2909
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_51705"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..293
                     /locus_tag="VEA63_10625"
     CDS             <1..293
                     /locus_tag="VEA63_10625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD84502.1"
                     /translation="GVKAVLLGKRVTIGDARVVIVIAKAGEAQEAFLKASVAMTTSQF
                     QNHWRRLFMTGGGSAPKVVETEADAVKLAAETPGAVVIGDSAALGDLATLAN"
     gene            658..1887
                     /locus_tag="VEA63_10630"
     CDS             658..1887
                     /locus_tag="VEA63_10630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD84503.1"
                     /translation="MSTNTSLLHRTLVFGALVLAALPASAAVTWKNIQLGGFASQGYL
                     INTGHNDYLGDTSEGTFDFREYAVNASWSKGKFRAGGQAFAQKLGEYGEDKVSLDWGI
                     VDYQATQWFGLRAGRVKMPRGLYNEALDVDSVRPFVLLPQSVYDARLRGFNAAFNGAM
                     VYGNVSLRNLGSVDYKLFYGDIPMSTSSGANDYFNNDAPFPNASIGMDAVYGGSLFWN
                     TPVSGLRAGYSYSAFQDFGADRIVSFVIPGVPPMEFIMYRATDLYERHLLSAEYTTGD
                     WIFATEIGRETAFFGIGFPATAPIGFFDIENLYGYLSVARRLNSRVEVGAYYSYSKEK
                     QLSVGIENGQIFPDAIQHDFAVSAKFDITDHLIAKLEGHYLDGAGKLFDTATKPQPFA
                     RRDDSWFMLAAKVTYSF"
     gene            1916..2329
                     /locus_tag="VEA63_10635"
     CDS             1916..2329
                     /locus_tag="VEA63_10635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD84504.1"
                     /translation="MNTPFKPLVFFAAALALACSSHAAPALASHAGLKGQTLDADGVK
                     AVLLGKRVTIGDARVVIVIAKAGDAQEAFLKTHVAMTTSQFQNHWRRLFMTGGGSAPK
                     VVETEADAVKLAAETPGAIAIADSGATGELAILAN"
     gene            2363..>2909
                     /locus_tag="VEA63_10640"
     CDS             2363..>2909
                     /locus_tag="VEA63_10640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MCP four helix bundle domain-containing protein"
                     /protein_id="HYD84505.1"
                     /translation="MKLLRSSKNFSFRQTILTVTVCFALVAIGVGALAFVSGGYTRQG
                     SRQTETLTSQFLPGLVSLTRLQDGALNLKSITLQFALARDEAGMNAQKQAFVVETEAV
                     SQSLAQLESLSTDDQSKALIADLGTSLTAYREVGEKFQTELRGGDFEKAMATLDQQVA
                     PAQQLVETNLDKLSEQYFARSK"
BASE COUNT          567 a         1045 c          759 g          538 t
ORIGIN      
        1 aaggcgtcaa agccgtcctc ctcggtaaac gcgtgacgat cggcgacgcc cgcgtcgtca
       61 tcgtcatcgc caaagccggc gaggctcagg aagcgttcct caaagcgagc gtcgccatga
      121 cgaccagcca gtttcaaaac cactggcgcc gtcttttcat gaccggcggc ggctccgcgc
      181 ccaaggtcgt cgagaccgag gccgacgccg tgaagctcgc cgccgagacg cccggcgccg
      241 tcgtcatcgg cgactccgcc gccctcggcg acctcgccac gctcgcaaac tgacgtctcc
      301 cgcaacggaa aaagacaagg cccggcgttc tcgcaacgcc gggctttttt gtgcccaccg
      361 ggccggtggc tgcagcgcgc cgccaccgta ttgaaaaact cattacgtgt tttcgccctc
      421 gctcgccgct cgcatgcgac cacagcggaa aatttcggcc gtttgttgca cggtttcccc
      481 cggtttccgt ttctcacgca agccggagtt ccgaggtggt ttgtgacaaa ttcgtgactc
      541 cacctcgatc cccgctgcgc cgatgcatga catgcgagcc aagtcgcggg cactcgatga
      601 acgagcattc ggcctgcagc tcggcaaccc aactttagca ccactttgat gttcaacatg
      661 agcaccaaca ccagcctcct ccatcgcacc cttgttttcg gcgcgctcgt cctcgccgca
      721 ctgcccgcgt ccgctgccgt cacttggaaa aacatccagc tcggcggctt cgcgagtcag
      781 ggatatctca tcaacaccgg tcacaacgac tacctcggcg acacgagcga gggcacgttc
      841 gactttcgcg aatacgcggt gaacgcctca tggtccaagg gcaagttccg cgcgggcggc
      901 caagcgttcg cgcagaaact cggcgaatac ggtgaggaca aggtcagcct cgactggggc
      961 atcgtggatt atcaagccac ccagtggttc ggtctgcgcg cgggccgcgt gaaaatgcct
     1021 cgcggcctct acaatgaagc actcgacgtc gattccgttc gtcccttcgt gctgctgccg
     1081 caaagcgtct acgatgcccg cctccgcggc ttcaacgctg ctttcaacgg ggcgatggtc
     1141 tacggcaacg tgagcctgcg caacctcggc agcgtcgact acaaactgtt ttacggcgac
     1201 atcccgatgt ccacctcctc gggcgccaac gactatttca acaacgacgc tccgttcccg
     1261 aatgccagca tcggcatgga cgctgtctac ggcggcagcc tcttttggaa cacgcccgtc
     1321 tcgggcctgc gcgccggcta ttcctacagc gcctttcagg acttcggcgc cgaccgcatc
     1381 gtgtcattcg tcatcccggg tgtgcctccg atggaattca tcatgtatcg cgcgaccgat
     1441 ctctatgagc gccacctcct gtcggccgaa tacaccaccg gcgattggat tttcgctacc
     1501 gaaatcggcc gcgagaccgc cttcttcggt atcggttttc ctgccaccgc tcccatcggc
     1561 ttcttcgaca tcgaaaatct ctacggctac ctctccgtcg cccgccgact caacagccgc
     1621 gtcgaggttg gcgcctacta cagctactcg aaggagaagc agttgagcgt cgggatcgaa
     1681 aacggccaga tcttccctga tgccatccag cacgacttcg ccgtaagcgc gaaattcgac
     1741 atcaccgatc acctgatcgc aaagctcgag ggacattacc tcgatggcgc gggcaagctg
     1801 ttcgacaccg ccaccaagcc gcagcccttc gcccggcgcg acgattcctg gttcatgctc
     1861 gccgccaaag tcacctactc gttctgagcc gctccacctc ctactggctg actccatgaa
     1921 cactccgttc aaacccctcg tcttcttcgc cgcagcactc gcgctcgcct gctcctcgca
     1981 cgccgcaccg gctctcgcca gccacgccgg cctcaagggc cagacgctcg atgccgacgg
     2041 cgtcaaagcc gtcctcctcg gtaaacgcgt cacgatcggc gacgctcgcg tcgtcatcgt
     2101 catcgccaaa gccggcgacg ctcaggaagc gtttctcaaa actcacgtcg cgatgaccac
     2161 cagccaattt caaaaccatt ggcgccggct ctttatgacc ggcggcggca gcgcccctaa
     2221 agttgtcgag accgaagccg atgcagtgaa gctggccgcc gaaacgcccg gcgccatcgc
     2281 gatcgccgat tccggcgcca ccggcgagct cgcgatcctc gccaactaat tttccaccgc
     2341 cagccgtttg cctccttttc caatgaaact ccttcgctcc tctaaaaact tcagttttcg
     2401 ccagacgata ctgaccgtca ccgtgtgctt tgcactggtc gccatcggcg tgggggcact
     2461 tgccttcgtc tccggcggct acacccgcca aggcagccgg caaaccgaaa cgctcaccag
     2521 ccagttcctg cccggcctcg tcagcctcac gcgtctccag gacggagcgc tcaatctcaa
     2581 gagcatcacg ctgcagttcg cgctcgctcg cgacgaagcc ggcatgaacg cccagaagca
     2641 ggcgttcgtg gtcgaaaccg aagccgtcag ccagagcctc gcgcaactcg agtcgctctc
     2701 caccgacgat caaagcaagg cgctgatcgc cgatctcggc acctcgctca ccgcctatcg
     2761 cgaagtcggc gaaaaattcc agaccgaact ccgcggcggc gatttcgaaa aagccatggc
     2821 gacgctcgac cagcaggtcg cgcccgccca acagctcgtc gaaaccaacc tcgacaaact
     2881 cagcgaacaa tacttcgccc gctcgaaag
//
LOCUS       DASPLT010001027         1085 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_176653, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001027 DASPLT010000000
VERSION     DASPLT010001027.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1085)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1085)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1085
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_176653"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1085
                     /locus_tag="VEA63_10645"
     CDS             <1..>1085
                     /locus_tag="VEA63_10645"
                     /inference="COORDINATES: protein motif:HMM:NF012686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD84506.1"
                     /translation="MNMFLPASDSVLPWTALLLGLWIPNFYYWGLNQYITQRTLGSAS
                     LAQGQKGIVFAAFMKLIIPFVIVVPGIIAFNLYAPEMRFEAATDTAASLTRYIKANPA
                     SAVVATADAPSDAQIAAHPDGRYLLAIYPDAAALSAVKTRNLFVLPITRAGFDSITPA
                     PFTIFTSEDHSWRHVNPNLAAEIDTYNAATTAAAAAAGAPTATEKLIAYKYDTALAQL
                     LARVLPQGKGWVGFVLAALLGAVVSSLAAMLNAASTIFAMDIYKKHVSPNATQKSIVL
                     LGRISVVVFTMVAVFLAPQLGNPKISNSIFTIIQEGQGFISPGILAVFVFGLLVRKAP
                     QVCGIVGLLTNVVAYGLLKFAVPQIQF"
BASE COUNT          187 a          434 c          261 g          203 t
ORIGIN      
        1 catgaacatg ttcctgcctg cgtccgacag cgtgctcccg tggaccgccc tcctgctcgg
       61 cttgtggatt cccaacttct attactgggg cctcaaccaa tacatcacac aacgcacgct
      121 cggctccgcc tccctcgcgc aaggccagaa gggcatcgtc ttcgccgcgt tcatgaagct
      181 catcatcccc ttcgtgatcg tcgttcccgg catcattgcg ttcaatctct acgccccgga
      241 aatgcgcttc gaagccgcga ccgacaccgc cgcgagcctc acgcgctaca tcaaggccaa
      301 ccccgcctcc gccgtcgtgg ccaccgccga cgcgccgtcc gacgcccaga tcgccgcgca
      361 tccggacggc cgttacctcc tcgcgatcta ccccgacgcc gccgcgctca gcgccgtgaa
      421 aacgcgcaat ctcttcgtgc tcccgatcac ccgcgcgggt ttcgattcca tcacgcccgc
      481 accgttcacg atcttcacca gcgaagatca ctcctggcgc cacgtgaatc ccaacctcgc
      541 cgccgaaatc gacacctaca acgccgccac caccgcggcc gcagccgccg cgggtgcccc
      601 gaccgccacc gaaaaactca tcgcctacaa atacgacacc gctctcgcgc aactcctcgc
      661 ccgcgtcctc ccccagggca aaggctgggt cggcttcgtc ctcgccgcct tgctcggcgc
      721 cgtcgtcagc tccctcgccg cgatgctcaa cgccgcctcc accatcttcg cgatggacat
      781 ctacaagaag cacgtgtcgc ccaacgccac acagaagagc atcgtcctgc tcggccgtat
      841 ttccgtcgtc gtgttcacga tggtcgccgt gttcctcgct ccgcagctcg gcaatccgaa
      901 gatcagcaac agcatcttca cgatcatcca ggaaggccag ggcttcatct caccgggcat
      961 tctcgcggtt ttcgtgttcg gcctgctcgt gcgcaaggcc ccgcaggtct gcggcatcgt
     1021 cggcttgctc accaacgtcg tcgcttacgg cctgctcaag tttgctgtgc cgcaaatcca
     1081 gttcc
//
LOCUS       DASPLT010001028         1333 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_299119, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001028 DASPLT010000000
VERSION     DASPLT010001028.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1333)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1333)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1333
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_299119"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            210..422
                     /locus_tag="VEA63_10650"
     CDS             210..422
                     /locus_tag="VEA63_10650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD84507.1"
                     /translation="MSVAEIKAEMAKLPPAEMVHLAAYAQHLARRNEPGYAAGLDVER
                     DQFEKGDRIAGAEIRRLAAELDHAGL"
     gene            851..>1333
                     /locus_tag="VEA63_10655"
     CDS             851..>1333
                     /locus_tag="VEA63_10655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009513069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination/repair protein RecA"
                     /protein_id="HYD84508.1"
                     /translation="MSKAAPAKNSTAVAAAPARDKNIDLALSAITKQFGEGSIMRLGS
                     NAKMKVETLSTGSLAIDLALGVGGLPKGRIIEIYGPESSGKTTFCLSVIAEAQKAGGL
                     AAFIDVEHALDPKYARVLGVNLDDLLVSQPDSGEDALNITETLIRSNSIDVIVIDSVA
                     A"
BASE COUNT          243 a          415 c          410 g          265 t
ORIGIN      
        1 tcggtggcga atttgatcgt cgccgaaatc gcaaaacgcc gcggcgtgca cctctcgttt
       61 ggcgaatact tgaaggccgg cacgccgatc gcagtgctga cgctggcttt gggcgtgtgg
      121 tggctgagcc ggtgaaaagc ggctaatggg acactcgcgc gttttgagaa tcagtttgtt
      181 ttcaatctga ccccacgtag agtggcgcca tgtcagtcgc cgagatcaaa gcagaaatgg
      241 ccaagctccc gccggcggag atggtgcatc tcgcggccta tgctcagcac cttgctcgtc
      301 gcaatgagcc gggctatgcg gccggcctcg atgttgagcg tgaccagttc gagaagggcg
      361 accgcattgc cggagctgaa attcgccggc tcgccgccga actcgatcac gcggggctgt
      421 gagcgcctac gactacgtgt taagggcgtc ggcggcgcgt gtggtcttgg gcagttcgaa
      481 acggttacgg cgggagatcc tctctgagtt ggagcgcctc gcccgcgagc cgtttagcaa
      541 acccgatttg gaggaaaccg gcccttcagg tcggacatat gccattcggg ttcgcgagcg
      601 gattatcctc acctactggg tggaccacgc cgccagggag gtgtgcgtga ttcgcgtcga
      661 gatttgctga ccgctctgcg gagggtgcag ccgctggtaa acggtcatcg gcccaactgc
      721 gcaattttcc gcgcacgtcg ggcttgcttc ttcgcggcgg gaggggtcag cgttgacgga
      781 catttcgccg tttccccgct cggctgtccg cttcgtccgc tatttttctt caacgtccga
      841 actcgttccc atgtccaaag ccgctcctgc caagaactcc accgctgtcg ctgctgcgcc
      901 ggcgcgcgac aagaacatcg atctcgcgct ctcagccatc accaagcagt tcggcgaagg
      961 ctcgatcatg cggctcggca gcaatgccaa gatgaaggtc gaaacgcttt cgaccggttc
     1021 cctcgcgatc gacctcgcgc tcggcgtcgg cggcctcccg aagggccgca tcatcgaaat
     1081 ctacggaccg gaatcctccg gcaaaaccac gttctgcttg agcgtgatcg ccgaggcgca
     1141 aaaagccggc ggactcgcgg cgttcatcga cgtcgagcac gcgctcgacc cgaaatacgc
     1201 ccgcgtgctc ggcgtgaatc tcgacgacct gctcgtttcc caacccgaca gcggcgagga
     1261 cgcactcaac atcaccgaga cgctgatccg ctcgaacagc atcgatgtga tcgtgatcga
     1321 ttctgtcgcg gcg
//
LOCUS       DASPLT010001029         2337 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_277927, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001029 DASPLT010000000
VERSION     DASPLT010001029.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 2337)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2337)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2337
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_277927"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..444)
                     /locus_tag="VEA63_10660"
     CDS             complement(<1..444)
                     /locus_tag="VEA63_10660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD84509.1"
                     /translation="MIGSEAEPVAFVSSLTAGHEPLRRDLFELGLELGRYVWVCEHPD
                     CRPDLNPRQETPLTIMDVLLERVRASHIYLLVLGGERHGTALRVGDRESNVSHFEAEL
                     FQAALHGKRVELFVRRGFSPGPRLSKLLAILGWALPSTAWRTPMND"
     gene            complement(450..1157)
                     /locus_tag="VEA63_10665"
     CDS             complement(450..1157)
                     /locus_tag="VEA63_10665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD84510.1"
                     /translation="MEIYILRSWLCFKDVKLAALPGVKKALSSGAFTPESFAAAAKAL
                     GKNPTELGHALKLGAGLVPLSAATDSAVTGFRVKDFAHRVWVQLTTRVIARKFNWEHD
                     DLAKVYDSWYEFFRYVRSLAEEAMFYREADTALANEVRSLVIDELLDAIMRPHLTNHP
                     RRFRHWLEHDARNTQEFASLEPQELQRKYPKFALLQADLIETQNKISGVEARLVSLLF
                     PQPSSQDGRTPPRFATE"
     gene            1269..1565
                     /locus_tag="VEA63_10670"
     CDS             1269..1565
                     /locus_tag="VEA63_10670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD84511.1"
                     /translation="MFTCTPDDTGSGLVPPTSRKVCNTAGVVSEANQECSNMSSKHHD
                     RGQRDAAKGYGNYSAPHGVVKELVFGWGREGRRMREENRSYQKGYVNGKKQRSR"
     gene            complement(1572..1775)
                     /locus_tag="VEA63_10675"
     CDS             complement(1572..1775)
                     /locus_tag="VEA63_10675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD84512.1"
                     /translation="MAKSGRTYINQSVSFPPELLDTAKERAENLGLSFSAYVQKLLEK
                     DLTERGAIVFAEKASAPKRKSDA"
     gene            1902..2054
                     /locus_tag="VEA63_10680"
     CDS             1902..2054
                     /locus_tag="VEA63_10680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD84513.1"
                     /translation="MPRSLYRRLAAHAARQNSDIETLIVAAVKADIAAACGPVATNSS
                     KSKKTK"
     gene            2051..>2337
                     /locus_tag="VEA63_10685"
     CDS             2051..>2337
                     /locus_tag="VEA63_10685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD84514.1"
                     /translation="MKIPASLLGLGFLLAVTAGCETTNNAPPQATVQAPALPAPVQST
                     AAAPGALSYGMVTGRVVKGVTTQFDVLELFGGPSTMTTDKDGSEVWMYDKTA"
BASE COUNT          474 a          672 c          734 g          457 t
ORIGIN      
        1 gtcgttcatt ggcgttcgcc acgccgtgga aggcagcgcc catccgagaa tcgcgagcag
       61 tttagagagg cgcggaccgg gagaaaatcc gcggcgcacg aacagctcca cgcgtttgcc
      121 gtgcagcgcg gcttgaaaga gttcggcctc gaagtgcgaa acgttcgatt cgcgatcgcc
      181 gacacgcagg gcggtgccgt gacgttcgcc gccgagaacg aggagataaa tatgggaggc
      241 gcgcacgcgt tcgagcagca catccatgat ggtcagcggc gtctcttgcc gcggattgag
      301 atcgggccgg cagtcgggat gttcgcagac ccagacgtag cggccgagtt cgaggcccag
      361 ctcgaacaga tcccggcgaa gcggctcgtg ccccgcggtg agtgaactga cgaaagcgac
      421 cggctctgcc tcggacccga tcatgaaagc tattctgtcg cgaaacgcgg aggtgtgcgg
      481 ccgtcctggg aagacggctg cggaaagagt aacgagacca gtcgcgcttc gacaccggag
      541 attttgttct gggtctcgat caagtcagct tggaggagtg cgaacttcgg atacttgcgc
      601 tgcagctcct gtggttcgag cgatgcgaat tcctgcgtgt tccttgcgtc gtgctcgagc
      661 cagtgacgga agcgccgcgg gtgatttgtc agatgggggc gcatgatggc atcgagcagt
      721 tcatcgatca ccaggctgcg aacttcgttg gccagcgcgg tatcggcttc gcgataaaac
      781 atcgcttctt ccgcgagcga gcgcacgtat cggaaaaact cataccaact gtcgtagacc
      841 ttcgcgagat cgtcgtgctc ccagttgaac ttgcgggcaa taacgcgggt ggtgagttgc
      901 acccacacgc gatgggcgaa atccttcacg cgaaatccgg tgacggccga atcggtggcg
      961 gcgctgagcg ggacgaggcc ggcgccgagc ttgagggcgt ggccgagttc ggtgggattt
     1021 ttgcccaagg ctttcgcggc ggcggcgaaa ctctccgggg tgaaggcgcc gctcgataat
     1081 gcttttttca cgcccggcag agcggcgagt ttgacgtcct tgaaacaaag ccaggagcgc
     1141 aaaatataaa tttccatggc gctgaagtgc cgtggcgcgc gttttcttcc attattttct
     1201 cccggcgtct tcattcgcgt gcgtgtcgta gctttccgcc ccgtcgcagt caaaccggcg
     1261 ctctgttatt gtttacgtgc actccggacg acaccggctc ggggctggtg cccccgacaa
     1321 gccgcaaggt ttgcaacacc gctggtgtcg tctcggaggc aaaccaggag tgcagtaaca
     1381 tgagcagtaa acatcatgat cggggtcagc gtgacgcggc gaagggctac gggaactaca
     1441 gcgccccgca cggcgtcgtg aaggaactcg ttttcggctg gggccgcgaa ggccgccgca
     1501 tgcgcgagga gaatcgcagc tatcaaaaag gctacgtcaa cggcaagaag cagcgcagcc
     1561 gttgaacgcg ttcaggcatc ggacttccgc ttcggagccg atgccttttc cgcaaacacg
     1621 atcgcacctc gctcggtgag atccttctcc agcaattttt gcacataggc gctgaaagag
     1681 agcccaaggt tctctgcgcg ctctttggcg gtgtccaaca gctcgggtgg gaatgaaacg
     1741 ctctgattga tgtaagtcct gccggatttg gccatgcgcc gagtgtgcga attctgccgt
     1801 gaaatccaag acccagttta gcaattgtta ttgaccgtta ttaaccaatc ataacgatga
     1861 cttctcgtat gccagatgcc ccgcgtcgcg tccgtgttct cttgccgcgt tcgttgtatc
     1921 ggcggctggc agcgcacgcg gcgcggcaaa acagcgacat tgaaacgctg atcgtcgcag
     1981 ccgtgaaagc ggacatcgcg gcggcgtgcg gtcctgtcgc caccaactcc agcaaatcaa
     2041 aaaaaacgaa atgaagattc cagcttcact cctcggtctc ggcttccttc ttgcggtcac
     2101 tgcaggttgc gaaaccacga ataacgcgcc accgcaggcc accgtccaag ccccggcttt
     2161 gccggcgccg gtgcagtcaa ccgcggcggc gcccggcgcg ctttcctatg gcatggtgac
     2221 gggccgcgtg gtcaaaggcg tcacaacgca gttcgacgtt ctcgaactct tcggcgggcc
     2281 gtcgacgatg accacggaca aggacggcag cgaagtgtgg atgtatgaca agaccgc
//
LOCUS       DASPLT010001030         2053 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_35852, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001030 DASPLT010000000
VERSION     DASPLT010001030.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 2053)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2053)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2053
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_35852"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..203
                     /locus_tag="VEA63_10690"
     CDS             <1..203
                     /locus_tag="VEA63_10690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD84515.1"
                     /translation="NDGVTAATMNLNTAQSVPNVGTQEWLRDRDFTATPITPQARYLV
                     VSTGAAPNDRPNEFAFVGYVVP"
     gene            complement(289..1194)
                     /locus_tag="VEA63_10695"
     CDS             complement(289..1194)
                     /locus_tag="VEA63_10695"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HYD84516.1"
                     /translation="MELRHLRYFVAVAEALSYRGAAERLHVAQPALSKQIQDLEAEVG
                     ARLLNRNTGGVSLTDAGAVLLDEARDILERVEMAAAAARDAASGKGGRLTIGSLGAVS
                     ASFLPASLAAFRERFPGVEINLHESTMPEQITELRRGVIQLGFAIEQAVEIPPDLETL
                     EIQTVRMAVALGPEHRFAGKSKVSLAELAHEPLLCVGIADRYDLHRKMIESVFDARGI
                     RHRPLKRVNGLESLVALIAGGHGVSLLLPFPLSGRKREIAFKRVAEDGDDLAVRMLAV
                     WRARDGSKLAQNFVEVLRAQRRAAR"
     gene            1276..1791
                     /locus_tag="VEA63_10700"
     CDS             1276..1791
                     /locus_tag="VEA63_10700"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HYD84517.1"
                     /translation="MFAASFSHGPACDALMAVARDRSPVGELQCHALFGLFETCASLS
                     HALKQELARHAHTESGFNVLGYVLHHASEVVTPRNIADGLGLSLQTVTAILGRLEVSG
                     LIARERASEKKRDFTIKATAAGRQAFASALSHQLASITRLMSPLDPRDLAALDVTCAR
                     LREISTQLPTS"
     gene            1803..>2053
                     /locus_tag="VEA63_10705"
     CDS             1803..>2053
                     /locus_tag="VEA63_10705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007362209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD84518.1"
                     /translation="MSPSLHRSSRLRSGFHAASLASVALLFAACGKQQNAAPATPSAM
                     EVGVLVVQPETVALSREVPGRLSPYRIAEVRARINGIVQ"
BASE COUNT          354 a          794 c          549 g          356 t
ORIGIN      
        1 tgaacgacgg ggtgacggcc gcgaccatga acctcaacac cgcgcagtcc gttcccaacg
       61 tcggcactca ggagtggctc cgcgatcgcg acttcaccgc gacgcccatc acgccgcaag
      121 cccgctacct tgtggtgagc acgggcgcgg ctccaaacga ccgccccaac gagttcgcct
      181 tcgtcggcta cgtcgtgcca taacccgcga tccgatctcg cgcatcccgc gcaaagccct
      241 cgagcctccg gccgccagcc ggaggctctt tttcttttcg cctgcgattc aacgcgccgc
      301 ccgccgctgc gcgcgcagga cctcgacgaa attctgcgcg agcttcgaac catcgcgcgc
      361 acgccagacc gcgagcatgc ggaccgcgag atcgtcgcca tcctccgcca cgcgcttgaa
      421 cgcgatttca cgcttccgac ccgacagcgg aaacgggagc agcaacgaca cgccatgccc
      481 gccggcgatc aacgccacga gcgactccag tccgttcacg cgtttcaatg gtcggtgccg
      541 gattccgcgc gcatcgaaca ccgactcgat catcttgcgg tgaagatcgt agcggtccgc
      601 gattcccacg cacaacagcg gttcatgcgc cagctccgcg agcgacactt tgcttttgcc
      661 cgcaaagcga tgctccggcc cgagcgccac cgccatccgc accgtctgga tttccaacgt
      721 ctccaagtcc ggtggtattt cgacggcctg ctcgatcgcg aaaccaagtt gaatcactcc
      781 ccgcctcagc tcggtgatct gctccggcat cgtcgactcg tgcaaattga tctcgacgcc
      841 gggaaaccgc tcgcgaaacg ccgcgagcga cgccggcaga aaactcgccg acaccgcgcc
      901 gaggctcccg atggtcaacc gcccgccctt ccccgacgcc gcgtcgcgcg ccgccgccgc
      961 cgccatctcg acgcgctcca ggatatcccg cgcttcgtcg agcaacaccg ctcccgcgtc
     1021 ggtcaaggac acgccgcccg tgttgcgatt caacagccgc gcgcccacct ccgcctcgag
     1081 atcctggatc tgcttgctca acgccggttg cgccacgtgc agacgctccg ccgccccgcg
     1141 gtagctcaac gcctccgcca ccgccacaaa gtatcgcaga tgccgtaatt ccatcccggc
     1201 gctgatactt tatacgtatc agcctgcaaa caacaaagtc acacgtcacg gcgcgcgatc
     1261 cgctatgatg ctgcgatgtt tgccgcctcg ttcagccacg gtcccgcctg cgacgccttg
     1321 atggcggtcg cacgggatcg ctcgcccgtc ggggagctcc aatgtcacgc cctcttcggt
     1381 ctgttcgaaa cctgtgcatc gctcagccac gcgcttaaac aggagctcgc ccggcacgcg
     1441 cacaccgagt cgggcttcaa cgtgctcggc tacgtgcttc atcacgcctc cgaggtcgtc
     1501 accccgcgaa acatcgccga cggccttggc ctttccctgc aaacggtgac cgcgattctc
     1561 ggccggctgg aagtgtcggg actgatcgcg cgcgaacgcg cctccgaaaa aaagcgcgac
     1621 ttcacgatca aggccacggc cgcgggtcga caggcattcg cctccgccct ctcgcaccag
     1681 ctcgcttcga tcacgcgcct gatgtcgccg ctcgatccgc gcgacctcgc ggccctcgac
     1741 gtcacctgcg cccggcttcg cgaaatttcc actcaactcc cgacctccta atccactcct
     1801 ccatgtctcc ctcactccac cgctcatcgc ggctccgttc tggatttcac gccgcatcgc
     1861 tggcgtctgt tgccttgttg ttcgccgctt gcggtaaaca gcagaacgcc gctcccgcca
     1921 ccccttcggc gatggaagtc ggcgtgctcg tcgtccagcc cgaaacggtc gcgctgtccc
     1981 gggaagtgcc cggacggctt tcgccctatc gcatcgccga ggttcgcgcg cgcatcaacg
     2041 gcatcgtgca aaa
//
LOCUS       DASPLT010001031         3669 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_257987, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001031 DASPLT010000000
VERSION     DASPLT010001031.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 3669)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3669)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3669
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_257987"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>3669
                     /locus_tag="VEA63_10710"
     CDS             <1..>3669
                     /locus_tag="VEA63_10710"
                     /inference="COORDINATES: protein motif:HMM:NF024603.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IPT/TIG domain-containing protein"
                     /protein_id="HYD84519.1"
                     /translation="VITPFVVPILNIEEISPAADQVARLGQPVEIRFTLPVNPYTITE
                     DVDNDGLLDAAEDLNVNNRIDAGEDVNGNGTLDLAEDLNGDGVIDTNVRIYRDADGNG
                     FLDEGEKENEDRVPGRLETNFTATAAVVVFTPTDNWQPASRYFVEVTSPPLDQKRPLD
                     PFDPATLPGVRELGTAALTSDLLASFTTATVASNTTVSVDSVQPRYNVATRTNTLLIA
                     GSGFSAASSVTIGDVAFAAGNGALHWIDAGKLELTLPPLDGRDLGMAAVKVANTDGTS
                     SIRYGAMVYVSTVQVYEISRNTSGPGGGIRVTLTGAGFLPGLTQVFVNGVAAEDVRVE
                     NVNTLSFIVPAGEFGTVDISVTVGGSTAVLPRALTYTLPAVPPSGNLQLKAIARLELD
                     GDLLLAMSKDEANATVLQTVDVSDPVDLIGLDSLPYTSGATFYYDAQTQFAYQGRANQ
                     VIRIDTAIPDAMQVVDSVTTAAGSTVRAIAAEGNLLVVGTDRALEFFTLTPFGGYLKS
                     RELPIEGGVGVLALDGTFLLHGQRIGGEELLCIRDLETSDFAIRGSVVLASAATALQL
                     GTNIAYLACKDAGVAQIVDFTARDLPVTLGYVNPRDDFADGAAPVLDLFVDRSTMFLA
                     CGAGGVQVFDITNPATPVFKQYGQVSGAATAVRATESFIFAGTSLGDILILPYSGLSV
                     SGVVPAYGSAAALESSVSVRFSGILQAPIDSTLFSLRDAANNPVAGNLVHSIEGNSSM
                     LTFRPDSLLQPNTDYAVLLAAGLVDVAGQVLSNDFSSRFRTVAVAGAIQPAITSISPA
                     YGSIAGGEVVTIIGRNLRDDTQVFFGAMAATDVVVSDNGTRLTVTAPAGAGVMSIRVV
                     NPGGLSENALYAFTYLAVPQVTSVTPARGSFVGGSNVTIRGSDFFPGTQVLFSGREAR
                     NVVVHDSALLTCETPDGVVGFADVEVRNPLRVVEGSTLSRGYLFTVPRTALTDTLRTP
                     DFIVTSEGVPELILGTSGSQLVIYNAFSPLNPVKSGSFNFGSRTPKHVSHDGDLAYVA
                     LGDRLDLIDLVDFEFPSQFLSKAFSTNTDLRKTVVLDSLLIAWDAQQNQLIVLDRETP
                     DHPVVAEHAVAPGETIRDILALEDSGRQFGVLIESDEHFSFAVLDVSTDPLVEVRRFQ
                     LPDGLRATTVYGRRLIATSATAISMVDLDGIEPMTQLLAGNYQSALIRTGVLFAAKAD
                     AVEVFDIAEPDAPELKTVV"
BASE COUNT          658 a         1098 c         1165 g          748 t
ORIGIN      
        1 cgtcatcacg cccttcgtcg tgccgatcct caacatcgag gagatcagcc cggctgccga
       61 tcaagtcgca cggctcggcc agcctgtgga gattcgtttc acgctacccg tgaaccccta
      121 caccatcacc gaggacgtcg ataacgacgg tctcctcgac gccgcggaag atctgaacgt
      181 caacaacagg atcgacgctg gagaggacgt gaacggcaac ggcacattgg atcttgcgga
      241 agacctcaac ggcgatggcg tgattgatac caacgttcgc atttatcgcg acgctgacgg
      301 caatggtttc ctggatgagg gtgaaaagga aaacgaggac cgtgtgcccg gtcggttgga
      361 gacgaacttt accgccacgg cggctgtcgt ggtgtttaca ccgacggaca attggcagcc
      421 agcttcgcgt tatttcgtcg aggtcacgtc gccgcccctc gatcagaagc ggccgctcga
      481 tccgttcgac cctgcgacgt tgcccggcgt tcgtgaactg ggcacggccg cactgacatc
      541 ggacctgctt gccagcttca ctacggcgac ggtcgccagc aataccacgg tcagcgtcga
      601 tagcgttcag ccacgttaca acgtcgctac ccgaacgaac actctgctca tagcgggaag
      661 cggattcagc gccgcgtcgt ccgtgaccat tggcgacgtc gcgtttgcgg cgggaaatgg
      721 agctcttcac tggatcgacg ccgggaagct cgagcttacg cttccgccgc tcgatggtcg
      781 cgacctcggc atggccgccg tgaaggtggc caacaccgac ggcacctcga gtattcgcta
      841 tggcgcgatg gtctatgttt ccacggtcca agtgtatgaa atttcgcgta acaccagcgg
      901 accgggcggc ggcatccgcg tgacgctcac cggcgccggt tttctgccgg gcctcacgca
      961 ggtcttcgtc aacggagtgg cggcggagga tgtgcgcgtc gagaacgtca acacgttgag
     1021 cttcatcgtg ccggccggcg aattcggcac cgtcgacatc agcgtgacgg tcggcggaag
     1081 cacggcggtg ctgccgcgag cgttgaccta cacattgccc gcggtgccac cgagcggaaa
     1141 tctgcagctc aaggcgatcg cgcgcttgga gctggatggc gacttgttgc tcgcgatgtc
     1201 gaaggacgaa gcgaacgcga cggtgctgca gacggtcgat gtcagcgatc cggtggacct
     1261 aatcgggctc gactcgctgc cctacaccag cggggcaact ttctattacg acgcacaaac
     1321 gcagttcgcc tatcaaggac gggcgaatca ggtgattcgc atcgataccg cgatcccgga
     1381 tgcgatgcag gtggtcgata gcgttaccac tgcggccggc tcgaccgttc gagcgattgc
     1441 cgccgagggc aacctgctcg tggtcggcac tgatcgtgcg ctcgagttct tcacgctgac
     1501 accgttcggc ggctacttga aatcgcgcga gctgccgatc gaaggcggag tgggggtgct
     1561 cgcattggac ggaacgtttc tgctgcacgg gcagcgcatc ggcggtgagg agctgctctg
     1621 tattcgcgat ctggagacat ccgatttcgc cattcgcggc tccgtggtgc tggcgtcggc
     1681 agcaaccgcg ctgcaattag gcacgaacat cgcctacctt gcgtgcaagg acgcgggtgt
     1741 ggcgcagatc gtggacttca ccgcgcgcga cctgccggtg acgcttggtt acgtgaatcc
     1801 acgggacgat tttgcggacg gggcggcacc cgtgctcgat ctgttcgtcg accggtcgac
     1861 gatgttcctc gcctgcggtg cgggcggcgt gcaggtcttc gacattacga atccggccac
     1921 gcctgtattc aaacaatacg gacaggtgag cggggcggcg acggctgtgc gcgcaacgga
     1981 atcatttatc tttgccggca cctcgctggg cgacattttg atcctgccct acagcgggct
     2041 gtccgtcagt ggagtcgttc ccgcctacgg cagcgcggcc gcgctcgaga gttcggtgag
     2101 cgtgcgtttc agcggcatcc tccaggcgcc gatcgactcg acacttttca gtttgcgcga
     2161 cgctgcaaac aacccggtgg ctggaaactt ggtgcattcc attgagggca acagctccat
     2221 gctgaccttc cggcccgaca gcttgctgca gccgaacacc gattacgccg tcttgctggc
     2281 ggcgggcctg gtcgatgttg ccggacaggt tttgagcaac gatttctcca gccgtttccg
     2341 aacggttgcg gtagcgggtg cgatccagcc ggcgattacg agcatctcgc cggcatacgg
     2401 ttcaatcgcg ggcggcgagg tggtgacaat tattggacgt aatctgcgcg acgacacgca
     2461 ggtcttcttc ggggctatgg cggcgaccga tgtggtcgtg tctgacaacg gcacgcgtct
     2521 gaccgtgacg gcgccagccg gggcgggggt gatgtcgatt cgcgtggtga atccaggcgg
     2581 cttgtcggag aacgcgcttt acgcgtttac ttatctcgcc gtgccgcagg tcacgtcggt
     2641 gacgccggcg cgcggtagtt ttgtcggagg cagcaacgtt acgattcgcg gctcggactt
     2701 tttccctggc acacaggtgc tgttttccgg ccgggaagcg cgtaacgtgg tggtccacga
     2761 ttcagccctg ttgacctgcg aaacgcccga cggcgtcgtc gggtttgcgg atgtcgaagt
     2821 gcgaaatcct ctgcgtgtcg tcgaaggatc gaccctttcc cgcggctacc tgtttacggt
     2881 gccgcgaacc gcactcaccg atacgttgcg gacgcccgac tttatcgtaa cgagcgaagg
     2941 cgttccggag ttgatcctag gcacgtctgg ttcgcagctc gtaatctata acgccttctc
     3001 gccgctcaat ccggtcaaat cgggctcgtt caacttcggc agccgcacgc cgaaacacgt
     3061 ttcacacgat ggcgatctgg cctacgtggc tctgggagat cggctcgact tgatcgatct
     3121 cgtggacttc gagttcccct cgcagttcct gtcgaaggcg ttcagcacga atacggacct
     3181 tcgcaaaaca gtggtgctgg attcgctgct cattgcgtgg gatgcccagc aaaaccagtt
     3241 gatcgttctt gatcgcgaga cgcccgatca tccggtggtc gccgagcacg ccgtggcgcc
     3301 gggtgagact attcgcgaca ttctcgcgct ggaggacagt ggccgccagt tcggcgtgct
     3361 gatcgagtca gacgagcatt tcagtttcgc cgtgctcgat gtctcgacgg atccgctggt
     3421 ggaggtgcgg cgcttccaac tgccggatgg cctccgcgca acgacggtct acggccgccg
     3481 attgattgcc acatcggcga cggcgatctc gatggtcgat ctggacggca tcgagccgat
     3541 gacgcagctc cttgcgggaa attatcagag cgcattaatc cgcaccggcg tgcttttcgc
     3601 cgcgaaggcc gatgcggtgg aagtgttcga catcgcagag cccgacgcgc cggaattaaa
     3661 aaccgttgt
//
LOCUS       DASPLT010001032         2338 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_93192, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001032 DASPLT010000000
VERSION     DASPLT010001032.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 2338)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2338)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2338
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_93192"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..594
                     /locus_tag="VEA63_10715"
     CDS             <1..594
                     /locus_tag="VEA63_10715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HYD84520.1"
                     /translation="DKCGADYLGFIFHPKSPRHISLAHYRAMAPRLPERRKVAVSVEP
                     TIEELAAMQDAGFDYFQIHFRPETPDAELSAWSRLVGEKRLWLAPKLPPEKDVSPATL
                     AVAKFVLLDTFHAEGFGGSGKTGDWEKFARHQNANTHNFWILAGGLNPENIGEALRAS
                     GARFVDVNSGVEAAPGVKDIAKLKRFVVRLHESQTQA"
     gene            complement(568..1083)
                     /locus_tag="VEA63_10720"
     CDS             complement(568..1083)
                     /locus_tag="VEA63_10720"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009511363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HYD84521.1"
                     /translation="MTLFSDTFPTPLGLFSLTVDSAGALAATAFGDLLALQRRLPRSP
                     LCHIIDDKSATAAARKQVLAYLAGARRDFDLPLAPQGTVFQQRVWTALRRIPFGETRT
                     YRDLATALGNPGASRAVGRANATNPICLIVPCHRVIGADGSLTGFAFGEDLKRRLLDL
                     EGASRLRLALV"
     gene            complement(1080..>2338)
                     /locus_tag="VEA63_10725"
     CDS             complement(1080..>2338)
                     /locus_tag="VEA63_10725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HYD84522.1"
                     /translation="ARTAGFRACKKCHPDDFARGADPVLESVETVVAEIRDNPAAFPD
                     ARAIVRRLGFGATRTIELFRLHYHTTPSDLLLRARIDAAKARLLASSATLAEIAYSVG
                     FEALSVFHENFRELNGLTPAAYRGLTDTRSFELTLPPGYPLGYLRRALSRDPHSANER
                     LVNDTYTAALRLGGEATQLTLHLAPQTIHAELSHGSAVEAHAIVIGLLGLDEDAAAFA
                     RLAKKLGLARLVAGRAELRISRTPSLFDGLLWSIIGQQINFAFACLLKSRLTQLAGQP
                     LAHGLYVPPDPAAVARLEPRELLPLQFSRQKADYVIGTARLIVEGRLDLSALARMSAT
                     RAERTLLAIRGFGPWSVNYVMMRSLGFPDCVPLGDTGVTSGLQSLLKLDERPDIDATR
                     RLMAVFSPYRSLATAHLWQLNQPIPT"
BASE COUNT          444 a          587 c          920 g          387 t
ORIGIN      
        1 gacaaatgcg gcgcggacta cctcgggttt atctttcatc cgaagtcgcc gcggcacatc
       61 tcgcttgcgc attaccgcgc aatggcgccg cgattaccgg agcgacgaaa ggtggcggtt
      121 tcggttgagc cgacgatcga ggaactcgcg gcgatgcagg acgcgggctt cgattatttt
      181 cagattcatt ttcggccaga gaccccggac gcggagctga gcgcgtggtc gcggcttgtc
      241 ggggaaaagc ggctgtggct ggcgcccaag cttccgccgg agaaggacgt ttcaccggcg
      301 acgttggcgg tcgcgaagtt cgtgctgctc gacacgtttc acgccgaagg attcggcggc
      361 tcgggaaaga cgggcgattg ggagaaattc gcgcgtcacc agaatgcgaa tacgcacaat
      421 ttctggattc tcgccggcgg gttaaacccg gagaatattg gcgaagcgtt gcgcgcgagc
      481 ggagcgagat tcgtcgacgt caacagtggc gtcgaggcgg cgccgggtgt gaaagacatc
      541 gcgaaactga aacgtttcgt cgtgcggcta cacgagagcc agacgcaggc gtgatgcgcc
      601 ttcgagatcg aggagacggc gcttgagatc ttcgccgaat gcgaagccgg tgagcgatcc
      661 gtcggcgccg atcacgcgat ggcaggggac gatgaggcaa atcggattcg tggcgttggc
      721 ccggccaaca gcgcgcgagg cgccaggatt gccgagcgcg gtggcgaggt cgcggtaggt
      781 gcgcgtttca ccaaagggga tgcgccgcag cgccgtccat acgcgctgtt ggaacaccgt
      841 gccctgcggc gcgagcggaa gatcgaagtc gcgtcgcgcg cccgcgagat acgccagcac
      901 ttgtttgcgc gcggcggcag tggcggattt gtcatctatt atatgacaaa ggggcgaacg
      961 cgggaggcgg cgttggagcg cgaggaggtc gccgaaggcg gtggcggcga gggcgccggc
     1021 ggaatcgacg gtgagggaga agaggccgag aggcgttggg aaagtatcgg agaagagagt
     1081 catgtgggaa tgggttggtt gagttgccac aagtgagccg tggccaggct gcggtaagga
     1141 ctgaagacgg ccatgagccg acgcgtggcg tcgatgtcgg ggcgttcgtc aagcttcagc
     1201 agcgattgga gtccactggt gacgccggtg tcgccgagcg gcacgcagtc gggaaaaccg
     1261 agcgagcgca tcatcacgta attgacggac caaggcccga agccgcggat cgcgaggagc
     1321 gtgcgttcgg cgcgagtggc ggacatgcgc gcgagggcgg agagatcgag ccggccttcg
     1381 acgatgaggc gcgcggtgcc gatgacgtaa tcggctttct ggcgggagaa ttgtagcggg
     1441 agaagttcgc gcggttcgag gcgcgcgacg gcggcgggat cgggcgggac gtagaggccg
     1501 tgagcgagtg gttggccggc gagttgggtg agacgcgatt tgagcaggca agcgaaggcg
     1561 aagttgattt gttggccgat gatcgaccaa aggagtccgt cgaagagtga cggcgttcgg
     1621 ctgatgcgaa gttcggcgcg accggcgacg aggcgggcga ggccgagttt tttggcgaga
     1681 cgcgcaaagg cggcggcgtc ttcgtcgagg ccgagcaaac cgatgacgat tgcgtgagct
     1741 tcgacggcgg agccatggga gagctcggcg tgaattgttt gcggggcgag gtgcagcgtg
     1801 agctgtgtgg cctcgccgcc gagacgaaga gcggcggtgt aggtgtcgtt gacaaggcgt
     1861 tcgttggcgc tgtgcggatc gcggctgagc gcgcggcgaa gataaccgag cggatagccg
     1921 ggcgggagcg tgagctcgaa gctgcgggtg tcggtcaggc cgcgatacgc ggcgggtgtg
     1981 agaccattga gctcgcggaa gttttcgtga aagacggaga gcgcctcgaa gccgacgctg
     2041 taagcgattt cagcgagcgt agcggagctg gcgaggaggc gcgctttcgc tgcgtcgatt
     2101 cgcgcgcgaa gcagcaggtc tgagggcgtc gtgtgataat ggaggcggaa gagttcgatg
     2161 gtgcgcgtcg caccgaagcc tagccgacgc acaatcgcac gggcgtcggg gaacgccgcg
     2221 gggttatcgc ggatttcagc gacgacggtt tcgacggatt cgagaacggg gtcggcgccg
     2281 cgcgcgaagt cgtcagggtg acattttttg caggcgcgaa aaccggccgt gcgcgcgg
//
LOCUS       DASPLT010001033         1301 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_135666, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001033 DASPLT010000000
VERSION     DASPLT010001033.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1301)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1301)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1301
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_135666"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..374)
                     /locus_tag="VEA63_10730"
     CDS             complement(<1..374)
                     /locus_tag="VEA63_10730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HYD84523.1"
                     /translation="MSEAAKSATLLVIDDDAEIRYSLSRVLSSRGYQVTEAPSGEQGV
                     ALVKKGPAPDLVLLDVRMGGIGGIEALQLIRGANPRQLVILMTAFGTAQTAIEAMKYG
                     AFDYIMKPFDPAKVLTIAENAL"
     gene            674..>1301
                     /locus_tag="VEA63_10735"
     CDS             674..>1301
                     /locus_tag="VEA63_10735"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HYD84524.1"
                     /translation="MLGWSLNLFRIRGILLSVHASFFLLLAWIASEGWELDGWNGLLW
                     STGTLLAFFACVVLHELGHSFTAMHFGIGVRRILLMPIGGMAEFEAIPRQPSREFLIT
                     LAGPAVNFVIAAVLWATVGFPENGEYTLTTLGLAQLLLNANLVMGCFNLLPAFPMDGG
                     RILRALLASRMPYLRATRVAATIGKIVCVGGIVYGVWESWWMLALLFGF"
BASE COUNT          213 a          466 c          356 g          266 t
ORIGIN      
        1 ttgagcgcgt tctccgcgat ggtgagcacc ttcgccggat cgaacggctt catgatgtag
       61 tcgaacgccc cgtatttcat cgcctcgatc gcggtctgcg ccgtcccgaa cgccgtcatc
      121 aaaatcacca gttgccgcgg attcgccccg cgaatcaact gcaacgcctc gatcccgccg
      181 atcccgccca tgcgcacgtc gagcagcacg aggtccggcg ccgggccttt tttcaccagc
      241 gccacgccct gctcgccgct cggcgcttcg gtcacctggt aaccgcgcga cgacagcacg
      301 cgcgacagcg agtaacgaat ctcggcgtcg tcatcgatga caaggagggt ggcagacttg
      361 gcggcttcgg acatgcggcc ccagcgaaac gaacacgcgg ccaaagtaaa tagcgccgca
      421 cgccgcaggt ggaacccgac gtcccgttgg gttgtgtggg aggggcttta cgccccgact
      481 gatcaatcga atccacagcc catgcgcgcc tgcccgacca gcgctcgcgg caagtccgtg
      541 cacttccccg ccgcgttcac acccacgcgc cgcatcaaat aaatcctccc gcaccccaaa
      601 ccagttcgcc gcttccttcg tccgcgacaa aacaacactg gcgctttcag cgcacgcgct
      661 ttcattcccc ggaatgctcg gctggtctct caatctcttt cgcattcgcg gcattctcct
      721 ttccgttcac gcgagcttct tcctgttgct cgcgtggatc gccagcgaag gctgggagct
      781 cgatggctgg aatggcctgc tttggagcac cggcaccctg ctcgcgtttt tcgcctgcgt
      841 cgtgctccac gaactcggac actcgttcac cgcgatgcat ttcggcatcg gcgtgcgacg
      901 cattctcctg atgccgatcg gcggcatggc cgaattcgag gcgatcccac gtcagccttc
      961 gcgcgaattc ctcatcacgc tcgccggtcc tgccgtcaac ttcgtcatcg ccgccgtgct
     1021 gtgggccacg gtcggctttc cggaaaacgg cgaatacacc ctcaccacgc tcggtctcgc
     1081 gcaactcctc ttgaacgcga atctcgtcat gggctgcttc aatcttctgc ccgcgttccc
     1141 gatggacggc ggtcgtatcc tccgcgcgct tctcgccagc cgcatgccgt atttgcgcgc
     1201 gactcgtgtc gccgcgacca tcggcaaaat cgtctgcgtc ggcggcatcg tctacggcgt
     1261 ctgggaatcg tggtggatgc tcgcgctcct gttcggattc a
//
LOCUS       DASPLT010001034         1331 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_135670, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001034 DASPLT010000000
VERSION     DASPLT010001034.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1331)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1331)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1331
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_135670"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            283..984
                     /gene="queC"
                     /locus_tag="VEA63_10740"
     CDS             283..984
                     /gene="queC"
                     /locus_tag="VEA63_10740"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009508471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HYD84525.1"
                     /translation="MNVVVLCSGGMDSVAALYWAAREHAVAAVVSFDYGAKHNHRELP
                     FAAEHAAKLGVRHEVIAMDFVNRLFASDLLKSGGEIPEGHYEAENMKQTVVPFRNAIM
                     LSIACGFTESVGAEAVVIAAHGGDHAIYPDCREAFMQAMGEAMRLGTYAGVRLERPFI
                     TFTKGQIAAEGARLGVDFARTWSCYKGGALHCGKCGTCVERREAFAQAGLADPTVYEA
                     APPIPGKPSARESRE"
     gene            1007..>1331
                     /locus_tag="VEA63_10745"
     CDS             1007..>1331
                     /locus_tag="VEA63_10745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HYD84526.1"
                     /translation="MPIGVIREGENVLISEIFYSLQGEGVLTGVPSVFVRASGCNLRC
                     NWCDTPYASWNPEGEMRTIPQIVAEIQNHPSAHHVVLTGGEPMIAKDVRMLADAIKQM
                     GHHITI"
BASE COUNT          248 a          341 c          488 g          254 t
ORIGIN      
        1 aacagggcga gatcgggcgc gggggtcggc acgcgccatt cccagtcggg atgcgccgcg
       61 cggagatggg ctacgctctt cgtgacgcgc gcgggttgct cggggcgcat cgaagagccg
      121 tagtcggcgc attgcggatc atgaagaatc agcatctaaa tcgttctcgt tcttcgttct
      181 ctttctcgtt ctctccggcc ggaaggaaac gagaaagaga acgattacga gaacgagaac
      241 gattttgctt cggggcggcg gctccgcaaa cgtgagcgaa acatgaacgt ggtggtgctt
      301 tgttccggcg ggatggactc ggtcgcggcg ctttattggg ccgcgcgcga gcatgcggtc
      361 gcggcggtgg tcagcttcga ctacggcgcg aagcacaatc atcgcgaact gccgtttgcc
      421 gcggagcatg cggcgaagct cggcgtgcgg cacgaggtga tcgcgatgga cttcgtgaac
      481 cgcttgtttg cgtcggattt gttgaagagc ggcggggaaa ttcccgaagg gcattatgaa
      541 gcggagaaca tgaagcagac ggtcgtgccg tttcgaaacg cgatcatgct gtcgatcgcg
      601 tgcgggttta cggaaagcgt cggcgcggag gcggtggtga tcgcggcaca cggcggggat
      661 cacgcgattt atccggattg ccgggaggca ttcatgcaag cgatgggcga ggcgatgcgg
      721 ctcggaactt acgcgggcgt gcggctggag cggccgttta tcacgtttac caaaggacag
      781 atcgcggccg agggcgcgcg gctgggggtc gatttcgcgc ggacgtggtc gtgttacaaa
      841 ggcggtgcgt tgcactgcgg gaagtgcggc acgtgcgtgg agcggcgaga ggcgtttgcc
      901 caggcggggt tggcggatcc gacggtgtat gaggcggctc cgccgattcc cgggaagccg
      961 agcgctcgcg aatcgcgcga atgatcgctg attcggtttg tgattcgtgc cgataggcgt
     1021 gattcgcgaa ggagaaaacg tgctgatctc ggagatattt tattcgttgc aaggcgaagg
     1081 cgtgctgacg ggcgtgccgt cggtgttcgt gcgcgcgagc ggctgcaatc tgcgctgcaa
     1141 ctggtgcgac acgccttacg cctcgtggaa tccagaaggc gaaatgcgca cgatcccgca
     1201 gatcgtcgcg gagattcaaa accatccgtc ggcgcatcac gtcgtgctga ccggcggcga
     1261 gccgatgatc gcgaaggacg tccggatgct ggcggacgcg atcaagcaga tgggacatca
     1321 catcacgatc g
//
LOCUS       DASPLT010001035         1174 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_258031, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001035 DASPLT010000000
VERSION     DASPLT010001035.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1174)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1174)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1174
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_258031"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1032)
                     /locus_tag="VEA63_10750"
     CDS             complement(<1..1032)
                     /locus_tag="VEA63_10750"
                     /inference="COORDINATES: protein motif:HMM:NF013767.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HYD84527.1"
                     /translation="MPSFGGAETTSTATAIETKAAPAEAVSEDVMLSLHLEENRELLA
                     EFYGEAVDHLSQIEAALLALDQQPDNPEALNSIFRSFHTIKGNAGFLGLVPMHTLAHE
                     VESLLDLARNQKLRLNAAIITEILRSRDALGALTQQVALALEKGQLPDQIIPVGHLIR
                     AVKRLAENPQVGAPVATAAPAERASPTIDHHTPSVSPAEAPASPPVQTASAHAAPAAK
                     AAPSVVAPPSAKNTGGATVRVNTEKLDSLMDVVGELVIVQSQLLETARQHGENTGSPL
                     QRNVAQLSRITKELQHTAMSLRMIPIKQTFQKMERLARDLARDFGKKVQFATSGEDTE
                     LDRTVVEEIG"
BASE COUNT          200 a          350 c          391 g          233 t
ORIGIN      
        1 gccgatttcc tcgaccaccg tgcgatcgag ttcggtgtct tcgccgctgg tggcgaactg
       61 cacttttttc ccgaagtctc gcgcgagatc ccgcgccaga cgctccatct tttgaaaggt
      121 ctgcttgatc ggaatcatgc gcaacgacat cgccgtgtgc tgcagttcct tcgtgatgcg
      181 cgagagctgc gcgacattgc gctgcagcgg agaaccggtg ttttcgccgt gctgccgggc
      241 cgtttcgagc aattggctct gaacgatcac gagttcgccc acgacatcca tcaacgaatc
      301 aagtttctcg gtgttgacgc gcacagtcgc gccgccggtg ttcttcgcgc tcggaggagc
      361 aaccacgctc ggagccgctt ttgcagcagg cgcggcatgg gcggaagcag tttgaacggg
      421 cggggacgca ggtgcctccg cgggagacac gctgggggtg tggtgatcga ttgttggcga
      481 agcgcgttcg gccggagccg ccgtcgcgac cggagcgccg acttgcgggt tttccgcgag
      541 acgcttcacc gcgcgaatca ggtggccaac gggaatgatc tggtcgggca gttgcccttt
      601 ctcgagtgcg agcgcgactt gctgcgtgag cgcaccgagt gcgtcgcggc tgcgcagaat
      661 ctccgtgatg atcgcggcat tgaggcgcag cttctggttg cgtgcgagat cgagcagcga
      721 ctccacctcg tgcgcgagcg tgtgcatcgg cacgaggccg aggaagccag cgttcccctt
      781 gatcgtgtgg aacgaacgaa agatggagtt cagcgcctcg gggttatcgg gctgctggtc
      841 gagcgcgagc aaagccgcct cgatttgcga gagatgatca accgcttcgc cgtagaattc
      901 cgcgagcagc tcgcggttct cttccaggtg cagcgaaagc atcacgtctt cgctgacggc
      961 ttcagcgggc gcggctttcg tttcgatggc ggtggcggtg gacgttgttt ccgcacctcc
     1021 gaaagacggc acgagcgacc cactgcgcgc ggcatcaagg gccggcggca gccattccac
     1081 caaatgactc agcaaaccga gcgtcgcagc atcgaagggc ttcgcggcat cgagcagttt
     1141 ctccagcgca gcgtgcatcg cgctgatcgg ctga
//
LOCUS       DASPLT010001036         1787 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_155274, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001036 DASPLT010000000
VERSION     DASPLT010001036.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1787)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1787)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1787
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_155274"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..974
                     /locus_tag="VEA63_10755"
     CDS             <1..974
                     /locus_tag="VEA63_10755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HYD84528.1"
                     /translation="AALLEKIPLFPGDAKLATRAAGRDVLQPIAAELPLVIGGSADLY
                     GSTLNYIAADKDFDASNRAGRNIRFGIREHGMAAIANGIAYDGLFRPSVATFLVFADY
                     SRPSMRLAGLAKLPVVYVYTHDSIGVGEDGPTHQPVETVSALRLIPNFDVIRPADPEE
                     TAGAFAAAMERTDGPTLLALTRQTLPLLNDVDVQLRRTGVLKGGYIAVRETAPLTHIL
                     LSAGSEVQLAVAAAKTLGAGVRVVSMPSFYRFDQQPAEYRESVLPASCRKRVAIEAGV
                     TGLWNKYVGLDGKVIGIDRFGLSAPGSTVFKELGITTEAVVVAAQSL"
     gene            1455..>1787
                     /locus_tag="VEA63_10760"
     CDS             1455..>1787
                     /locus_tag="VEA63_10760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007359026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HYD84529.1"
                     /translation="MAFLLIKNLPRYDCLMEAAKLFPDLDPSACEVFLHLMQAGDDAF
                     RVVDSHLAEHNISQGRFMVLMLLLNKLTNCPHPRTPAELAELAHVTRATMTGLVDTLE
                     RDGLVKREP"
BASE COUNT          289 a          518 c          597 g          383 t
ORIGIN      
        1 cggcggcgtt gctcgagaag attccgcttt tcccagggga cgccaagctc gcgacgcgcg
       61 cggccgggcg cgatgtgctc cagccgatcg cggcggaatt gccgttggtg atcggtggga
      121 gcgccgactt gtatggctcg acgctgaact atatcgcggc ggacaaggat ttcgacgcgt
      181 cgaatcgcgc ggggcgcaac atccgtttcg gcattcgcga gcacgggatg gctgcgatcg
      241 cgaacggcat cgcgtatgac gggttgttcc gtcccagcgt ggcgacgttc ctggtgttcg
      301 cggattattc gcgtccgtcg atgcggctcg ccggcttggc gaagctgccg gtcgtttatg
      361 tttatacgca cgattcgatc ggcgtgggtg aggacggccc gacgcaccag ccggtggaaa
      421 cggtttcggc gctgcggttg attccgaatt tcgacgtgat ccggccggcc gatccggagg
      481 aaaccgcggg cgcgtttgcg gccgcgatgg agcgcaccga cggcccgacg ctgctcgcgc
      541 tcacgcgtca gacgctgccg ttgctgaacg acgtcgacgt gcagttgcgc cgcacgggcg
      601 tgctcaaggg cggctacatc gcggtgcgag aaaccgcgcc gttgacgcac atccttttgt
      661 cggcgggcag cgaagtgcag ctcgcggtcg ccgcggcgaa gacgctgggc gcgggcgtgc
      721 gcgtggtttc gatgccgtcg ttctatcgtt tcgatcagca gccggcggag tatcgcgaga
      781 gcgtgctgcc ggcgtcctgc cggaagcggg ttgccatcga ggcgggcgtg acggggctct
      841 ggaataaata cgtgggcctc gatggaaaag tcatcggcat cgatcgcttc gggctcagcg
      901 cgccgggatc aacggtgttc aaggaactcg gcatcacgac agaagctgtc gtcgtcgcgg
      961 cgcagtcgct gtagacgagc gcttcgcaaa atttgacgct tcggcccacg tgcagctgcg
     1021 cgtgggcctt ttttttgcgt ggcgaattcc aaggggtagt gcgctttgtc cctcaggcct
     1081 tgggattggt tgatcattcg ccgggctggc gtgcgctggt cgaactcagc taacagctca
     1141 tgcagtagac attgtcgggc cgtcgagaag cctgctcacc gaaagctgga gcccaacgtg
     1201 ctcgtcgggc ttgatgacgt ttcgctcgca aaaccgtccg gcgaggacgt cgagctctac
     1261 cttctacgca tgaggtcggc tcagggcgga tgggacgccc gcctttgcct ttttctcttt
     1321 gagttgcgac tcgtgagcgc gcgcggacca agaccagatt tgtaacaatt tgatggacga
     1381 ctgttatgat cgcttgcttt tttcgttagc ctcctaacta tcagccgcct tatctttttc
     1441 tgaccttacg cgtcatggcg tttctgctca tcaaaaatct tccgcgctac gactgtctga
     1501 tggaggcggc caagctcttc ccggacctcg atccgtcggc ttgcgaggtg ttcttgcact
     1561 tgatgcaagc gggagacgat gcgttccggg tggtcgattc gcatctggcg gagcataaca
     1621 tttcgcaagg ccgcttcatg gtgttgatgc tgttgctcaa caagctcacc aactgcccgc
     1681 atccgcgcac gccggccgaa ctcgcggagc tggcgcacgt cacgcgggcg acgatgaccg
     1741 gtctggtgga tacgctggag cgggacggct tggtcaagcg cgagccc
//
LOCUS       DASPLT010001037         2070 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_51825, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001037 DASPLT010000000
VERSION     DASPLT010001037.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 2070)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2070)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2070
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_51825"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..575)
                     /locus_tag="VEA63_10765"
     CDS             complement(<1..575)
                     /locus_tag="VEA63_10765"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HYD84530.1"
                     /translation="MSAGVAGGGPAVVRPIRHLRWWIGGMLFASTVINYVDRQTLTVL
                     SPFLKDEFQWTNEDYALIVIAFRVAYAFGMTALGRLMDRVGTRMGLAVTVACYSVVSI
                     FTSLASGLRSFIVFRFLLALAESPNWPAATKTVAEWFPKQERGWAVALFDSGSSIGAA
                     IAPALVIWLYHSFGGWRPAFLITGALGFVWIV"
     gene            complement(572..1411)
                     /locus_tag="VEA63_10770"
     CDS             complement(572..1411)
                     /locus_tag="VEA63_10770"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007363503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HYD84531.1"
                     /translation="MKKSSAPLRSETFGKLAVEVHADREALGRAAARAVAAYLRETIK
                     ERGEARVVFASAPSQDEFYATLVDARRTGIPFDWSKVTAFHMDEYLGPAANGPQSFRH
                     YLREHLLTYVKPGRFHGMEAEDRDTAAVCARYAALIKEKPIDLICAGIGENGHLAFND
                     PPVADFADKEIVKRVTLDTPCRRQQVNDGAFPKLAAVPRHALTLTVPVFARARRLSVQ
                     VPGPRKAEAVRATLKDPISTACPSTILRKHPNAILYLDTASAALALNEAQAEAAQPRR
                     SRG"
     gene            complement(1520..>2070)
                     /locus_tag="VEA63_10775"
     CDS             complement(1520..>2070)
                     /locus_tag="VEA63_10775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD84532.1"
                     /translation="AWKARLASDAAQAGRVRVVDGSFLTYWMVTDAMQPGDNLLASMG
                     GEERAKPKPGMRFEDPRGRMLSWSKTTSQVDTVMDLPEAKDGETVRYVYAALDADAER
                     EATLRVRGPGEVKVWVNGADVAAPADGDAWVLKLSPGRNGVLLKLMAPAEGEAWWFSA
                     EMPGEDLRHRKYRYDFYDAGGE"
BASE COUNT          408 a          800 c          534 g          328 t
ORIGIN      
        1 acgatccaaa cgaagcccaa cgcgcccgtg atcaaaaacg ccggacgcca gccgccaaaa
       61 ctgtgataga gccagatcac caacgccggg gcgatcgccg cgccaatcga ggaaccgctg
      121 tcaaacaaag ccaccgccca cccgcgctcc tgcttcggaa accactccgc caccgtcttc
      181 gtcgccgccg gccagttcgg cgactccgcc agcgccagca aaaatcgaaa cacgatgaaa
      241 ctccgcagcc cgctcgcgag cgacgtgaag atcgatacga cggaataaca cgccaccgtc
      301 accgccagcc ccatccgcgt gccgacgcga tccatcaacc gcccgagcgc cgtcatcccg
      361 aacgcatacg cgacacgaaa cgcgatcacg atcagcgcgt agtcctcgtt cgtccactga
      421 aactcgtcct tcaggaacgg cgagagcacc gtcagcgtct gtcggtcgac atagttgatg
      481 accgtcgacg caaacaacat cccgccgatc caccaacgca ggtgccggat cggtcgcacc
      541 accgccggcc cgccgcccgc cacgccggcg ctcatccgcg gctacgccga ggttgagccg
      601 cttccgcctg cgcctcgttc agcgccagcg ccgccgacgc cgtatccaga taaaggatag
      661 cgttcggatg cttccgcaga atcgtcgacg gacacgccgt cgaaatcgga tccttcagcg
      721 tcgcccgcac cgcttccgcc ttccgcgggc ccggcacctg cacgctcagc cggcgagccc
      781 gcgcaaacac cggcaccgtc aacgtcagag cgtggcgcgg caccgccgcg agcttcggga
      841 aagcaccgtc gttcacctgc tgacgccggc acggcgtgtc gagcgtcacg cgcttgacga
      901 tctccttgtc cgcaaaatcc gccaccggcg gatcgttgaa cgccaggtga ccgttctcgc
      961 cgatgcccgc acaaatcaga tcgatcggct tttctttaat cagcgcagcg tagcgcgcgc
     1021 aaaccgccgc cgtgtcgcgg tcctccgcct ccatgccgtg aaaacgcccc ggcttcacat
     1081 acgtgagcaa atgctcgcgc aggtagtgac gaaatgactg cggaccgttc gcagccggcc
     1141 cgagatattc atccatgtga aacgccgtca ccttggacca atcgaacgga atccccgtcc
     1201 gacgcgcatc caccagcgtc gcgtaaaact catcctgcga gggtgcgctc gcgaaaacaa
     1261 cccgcgcctc gccgcgctcc ttgatcgttt cgcgaaggta ggcggcgacg gcgcgagccg
     1321 ccgcacgccc cagcgcttcc cggtccgcat gcacttcaac cgcgagctta ccgaaggttt
     1381 cagaacgcaa aggagcagag gatttcttca tgatcggggt tgatgcgaag ctttctgtgt
     1441 ctctccgccc aaagcaaaga gagcgcccgt tcaagctgtt gaccaaccca cgatccgcgc
     1501 gaccgcagcg cgcatcggtt cactcaccac ccgcgtcgta aaaatcatac cgatactttc
     1561 gatgccgcag atcctcgccc ggcatctccg cggaaaacca ccacgcctct ccttccgccg
     1621 gcgccatcaa tttcaacagc accccattcc tccccggaga aagtttgagc acccacgcgt
     1681 cgccgtccgc cggcgcggcc acgtccgcgc cgttcaccca aactttcacc tcgcccggtc
     1741 cgcgcactcg caatgtcgcc tcccgctccg cgtccgcatc gagcgccgcg taaacgtagc
     1801 gcaccgtttc gccgtctttc gcttccggca gatccatcac ggtatcgacc tgcgacgtcg
     1861 tcttgctcca cgaaagcatc cgccctctcg gatcctcgaa acgcattccc ggcttcggct
     1921 tcgcgcgctc ttctccaccc atgctcgcca gcaggttgtc gcccggctgc atcgcgtccg
     1981 tcaccatcca gtaggtcaga aaacttccgt ccacgacgcg cacccgcccc gcctgcgccg
     2041 cgtcggacgc cagccgcgcc ttccacgcgc
//
LOCUS       DASPLT010001038         1068 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_93244, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001038 DASPLT010000000
VERSION     DASPLT010001038.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1068)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1068)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1068
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_93244"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..986)
                     /locus_tag="VEA63_10780"
     CDS             complement(<1..986)
                     /locus_tag="VEA63_10780"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HYD84533.1"
                     /translation="MKTVAAQIWAELPNLERGWSTRLVPFAREIVGDQVRTGLYVLVA
                     AVAVLLLIACANLSNLMLVRASARAHELAVRAALGAGRWQIARQLVGEALVLATIGGL
                     LGVLLALWAVDSFHTLPLPRAQEITIDLRVLGVALGTTFLTALLSTAVPALRAAQTRP
                     NEVLKNYLSQIAPRSRLRDAGVVIQIALSLTMLVGAALVARSFLKLLHVDPGFSAHNV
                     LTLSLRPATDHTRAVAFYDELVKRVSALPGVTASGLISILPFDQGNTSLNVFPLGPAA
                     LAPEASVQADWRLAHGDYFRTLRIPVLQGKTFANLPADEARRSVVISASLARA"
BASE COUNT          211 a          291 c          398 g          168 t
ORIGIN      
        1 gcgcgcgcaa gtgaagcgga gataacgacg gagcggcgtg cttcgtccgc cggaaggttg
       61 gcgaaggttt tcccttggag caccggaatc cgcagcgtgc gaaaatagtc gccatgcgcg
      121 aggcgccagt cggcttgcac cgatgcttcc ggcgccaatg ccgcgggccc aagtgggaag
      181 acgttgaggc tggtattgcc ctgatcgaac gggaggatgc tgatgagtcc gctggcggtc
      241 acgccgggca aagcagagac gcgtttcaca agttcgtcgt aaaaggcgac cgcgcgcgtg
      301 tggtcggtgg caggccgcag ggagagcgtg agaacgttgt gcgcggaaaa acccggatcg
      361 acgtgcagta atttcaggaa actgcgggcg acgagcgcgg caccgacaag catggttaac
      421 gagagagcga tttgaatgac gaccccggcg tcgcgcaggc gcgaacgcgg agcgatttgc
      481 gacaggtagt ttttaagcac ctcgttgggt cgcgtttgcg cggcgcgcag tgccggcacc
      541 gcggtggaga ggagcgcggt gaggaaggtt gtcccgagcg cgacgccgag cacgcggaga
      601 tcgatggtga tttcctgagc gcgcgggagc ggcagcgtgt gaaaactatc gaccgcccag
      661 agcgcgagca gcacgccgag caagccgccg atggtggcga gcacgagggc ttcgccgaca
      721 agctggcgtg cgatctgcca gcgtccggcc ccgagtgcgg cgcgcacggc aagttcgtgc
      781 gcgcgggctg atgcgcgcac aagcatgaga ttggaaagat tggcgcaggc gatgagcagc
      841 aggacggcga cggcggcaac gagcacgtaa aggccggtgc ggacttgatc gccgacgatc
      901 tcgcgtgcga acggcacgag gcgcgtgctc cagccgcgtt cgagattggg caactccgcc
      961 cagatttgcg cagcgaccgt tttcatctcg gcgtcggctt gatcgagagt aactccaggt
     1021 ttgaggcgac cgtagacttc gagatcgtgg ttgaggcggt cttggcgc
//
LOCUS       DASPLT010001039         1139 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_51853, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001039 DASPLT010000000
VERSION     DASPLT010001039.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1139)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1139)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1139
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_51853"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..224
                     /locus_tag="VEA63_10785"
     CDS             <1..224
                     /locus_tag="VEA63_10785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD84534.1"
                     /translation="FDGDVLKDHCGRAFRPGALYVDDGGSVLVEGMGTIALLDDRDLA
                     RIADEAQRFPTLVAAGVAEKYGFVPDPTP"
     gene            337..714
                     /gene="rpsL"
                     /locus_tag="VEA63_10790"
     CDS             337..714
                     /gene="rpsL"
                     /locus_tag="VEA63_10790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HYD84535.1"
                     /translation="MPTVNQLVRQGRSAKRPKSKVPALAGSPQKRGVCTRVYTTTPKK
                     PNSALHKVAKVRLTNGFEVIGYIGGEGHNLQEHSVVLIRGGRVKDLPGVRYHIVRGSL
                     DTQGVKDRKQARSKYGAKAPKSA"
     gene            728..>1139
                     /locus_tag="VEA63_10795"
     CDS             728..>1139
                     /locus_tag="VEA63_10795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007406077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD84536.1"
                     /translation="MPRRREVPKRQILPDPKFNNVDVAKFVNVLMTRGKKSVAERIVY
                     GALEILEKKGANQNFEGDEAIDTNANKGMLLFHRALKKVKPKLEVRSRRVGGATYQVP
                     VEVRPERAQALAIRWLISASRSRSEKTMAGRLSGE"
BASE COUNT          230 a          366 c          351 g          192 t
ORIGIN      
        1 atttcgacgg cgacgtgctg aaagaccatt gcggtcgcgc ttttcgcccg ggcgcgctgt
       61 acgtcgacga cgggggttcg gtgctcgtgg aaggcatggg taccatcgcg ctgctcgacg
      121 accgcgatct cgcgcgcatc gcggacgaag cgcagcggtt tccgaccctc gtcgcggccg
      181 gcgtggctga aaagtacggt ttcgtgcccg atccgacccc ctaaatcctt gcgactctag
      241 tctttcctct ttataatccg gcctcttttt tggcccctgg gcggctgatc cgagtacgcg
      301 acggccgccc ggttcgtttc tggaaacctt aaaaacatgc ctaccgtcaa tcaattagtg
      361 cgtcagggac gttccgcgaa gcgtcccaag tcgaaggtcc cggcgctcgc cggctcgccg
      421 cagaagcggg gagtctgcac ccgggtctac acgacgacgc ccaagaagcc gaactcggcg
      481 ctgcacaagg tcgccaaggt gcgcctcacg aacggcttcg aggtgatcgg ctacatcggc
      541 ggcgaaggcc acaacctgca ggagcactcg gtggtgctga tccgcggcgg tcgcgtcaag
      601 gacctgcccg gcgtgcgcta ccacatcgtg cgcggctcgc tcgacacgca aggcgtgaag
      661 gaccgcaagc aggcccgctc gaagtacggc gccaaggcgc cgaaatcggc ttaacaggaa
      721 gatctagatg ccccgccgca gagaagtccc gaagcgccag atcctgccgg atccgaaatt
      781 caacaacgtc gacgtggcga agttcgtcaa cgtcctcatg acgcgcggca agaaatccgt
      841 cgcggaacgt atcgtttacg gcgcactgga aatccttgag aaaaaaggcg ccaaccagaa
      901 ttttgaaggc gacgaagcca tcgacaccaa cgctaacaaa ggcatgctct tgttccaccg
      961 cgctcttaaa aaagtgaagc cgaaactgga agtgcgttca cgccgcgtcg gcggcgcgac
     1021 ctaccaggtc ccggtcgagg tgcggcccga gcgtgcccag gcgcttgcca tccgctggct
     1081 gatcagcgcg tcgcgctcgc gcagcgagaa gaccatggcc ggccgcctgt cgggcgagc
//
LOCUS       DASPLT010001040         1646 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_197638, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001040 DASPLT010000000
VERSION     DASPLT010001040.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1646)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1646)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1646
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_197638"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            101..187
                     /locus_tag="VEA63_10800"
     tRNA            101..187
                     /locus_tag="VEA63_10800"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:135..137,aa:Leu,seq:taa)
     gene            466..1236
                     /locus_tag="VEA63_10805"
     CDS             466..1236
                     /locus_tag="VEA63_10805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HYD84537.1"
                     /translation="MAAISPTPPRYNVLGVGVSALTLPRARDLVMAARFGDPRGYVCV
                     CAVHGISEARADPALRRIYNASYLTTPDGMPLVWLAPRGVERVYGPDLMLAVCDAGRA
                     HGLRHYFCGGRPGVAQQLAEKLTARFPGLMIAGVAAPPSDALSANELTALQNDLHRAQ
                     PDIVWVGFGTPRQERFMARHWRSFGAGLLVGVGAAFDFHAGRVPQAPRWMQRSGLEWL
                     FRLCMEPRRLAPRYLKTNPLFVLRVAAQKLRLIGYPID"
     gene            complement(1462..>1646)
                     /locus_tag="VEA63_10810"
     CDS             complement(1462..>1646)
                     /locus_tag="VEA63_10810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HYD84538.1"
                     /translation="YCPVERRHYHAALYDALGGALLLASLARDPRLAELSVMQLLALS
                     TLDPDKRDGLRQGSLF"
BASE COUNT          281 a          618 c          441 g          306 t
ORIGIN      
        1 gccagccatt cagccacgcc gccagcaccg cgccaccacg gtcgcgagcc cgagattgac
       61 ggtctccgca acgcctccga cattcgccca acctgaacaa gcccgggtgc tggaattggt
      121 agacagacgg gacttaaaat cccttgcctg taaaggcgtg cgggttcgac tcccgccccg
      181 ggcaccagcg tcaacgctac gctcggctaa accggaacca cgcaatgata ggagacaagc
      241 ccgcttgcag gcgatgagtg cctcacccgc gctccaagtg ccatcgcccg caagcaggct
      301 ccttgccttc gttcaccgca ccgttcggcg tcttcctaca tagttacgct ggactgcatc
      361 gttacggcta aacgctcgct ccaccggccg ctccgcgatt gcagcgagcc ttcgatccga
      421 caaagcggtt gctcgccgca cgcgtttgtt ctttctcggc gccgcatggc tgcgatctcg
      481 cccacgccac cgcgctacaa cgtcctcggc gtcggcgttt ctgctctcac gctgcctcgc
      541 gcgcgcgatc tcgtgatggc cgcgcgcttc ggcgatcctc gcggctacgt ctgcgtttgt
      601 gcggtccatg gcatcagcga agctcgcgcg gatcccgcgc tgcgccggat ttacaacgct
      661 tcgtatctga ccacgcccga cggcatgccg ctggtctggc tcgcgccgcg tggcgtcgaa
      721 cgcgtttacg gtcccgatct catgctcgcg gtgtgcgacg ccggccgcgc gcacggcctg
      781 cgtcactact tttgcggcgg ccgccccggc gtcgcccaac aactcgcaga aaaactcacc
      841 gcacgctttc cgggcttgat gatcgcaggc gtcgcggcgc cgccgtccga cgcgctttcc
      901 gccaacgaac tcaccgcatt gcaaaacgac cttcatcgcg cgcagcccga catcgtgtgg
      961 gtcggcttcg gcacgccgcg ccaggagcgc ttcatggcac gacattggcg ttccttcggc
     1021 gccggcctgc tcgtcggggt cggcgccgcg ttcgactttc acgccggccg cgtcccccaa
     1081 gcgccgcgct ggatgcagcg tagcgggctc gagtggctct tccgcctttg catggagccg
     1141 cgacgcctcg ccccccgtta tcttaaaacg aatccgctct tcgtcctccg cgtcgccgcg
     1201 caaaaactcc gcctgatcgg atatccaatc gactgatacg cctccgcgcc cggtcgaaca
     1261 caggcttgtc tcctacaatc gcccgcgctt cgccgattag gcgaagcacg cacttattcg
     1321 cccctcccaa ctcgcctccg aaaaatcagc gcagattagc ggttaaaaaa actctgccgt
     1381 ccgttcatca actttcccca gtcgagcacc gcgaggctcc tacacttgct acgtcttttc
     1441 gctaaaccgc ccgcgctttt ctcaaaacaa acttccctgc cgcaacccat cgcgcttgtc
     1501 cggatcgagt gtgctcaaag ccagcagctg catcacactc aactccgcca gccgcggatc
     1561 gcgcgcgagc gaagccagca acaacgctcc gcccagcgca tcgtagagcg ccgcgtggta
     1621 atgccgccgt tccaccggac aatagc
//
LOCUS       DASPLT010001041         1060 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_51905, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001041 DASPLT010000000
VERSION     DASPLT010001041.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1060)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1060)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1060
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_51905"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(36..>1060)
                     /locus_tag="VEA63_10815"
     CDS             complement(36..>1060)
                     /locus_tag="VEA63_10815"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: protein motif:HMM:NF006046.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HYD84539.1"
                     /translation="GANILSINQFDSRDVEELFRVAEAMGPYARREKRTRVLDGAILG
                     NLFFEPSTRTRVSFGCAFNLLGGHVRETTDIKASAISKGESLYDTARVISGYSDIIAM
                     RHPVAGSVEEFSRASRVPVINAGDGPSEHPSQALLDLYTIKKELNINGPVEGLRIAMI
                     GDLKYGRTVHSLGKLLCLYRNVHFTLVSPRELRMPGEIVERLRSAGHTVLETENMEEG
                     LHNNDTLYLTRIQEERFSDQREADIYRGRFRLNQEIYTRHCKPNTVIMHPLPRDSRAE
                     ANELDNDLNLHPSLAIFRQTDNGVLIRMALFALTLGVADEVSRHDEDVAWYTAKRFRK
                     PEPIGE"
BASE COUNT          162 a          315 c          355 g          228 t
ORIGIN      
        1 gattcccgcc ttcgcgggaa tgacgacggg attctttatt cgccgatcgg ttcgggtttg
       61 cggaaacgtt tcgccgtgta ccaggccacg tcttcgtcgt ggcggctgac ttcgtcggcc
      121 acgccgagcg tcagtgcgaa cagcgccatc ctgatcaaca cgccattgtc ggtctggcgg
      181 aaaatcgcga ggctgggatg caggttcagg tcgttgtcga gttcgttcgc ctcggcgcgc
      241 gaatcgcgcg gcagcggatg catgatcacg gtgttcggct tgcagtggcg cgtgtagatt
      301 tcctggttca ggcggaagcg gccgcggtag atgtcggctt cgcgctggtc gctgaagcgt
      361 tcttcctgga tgcgcgtcag gtacagcgtg tcgttgttgt gcaggccttc ttccatgttc
      421 tcggtttcga gcaccgtgtg gcccgcgctg cgcaggcgct cgacgatttc acccggcatc
      481 cgcaactcgc gcggcgacac cagcgtgaaa tgcacgttgc ggtacaggca cagcaacttg
      541 ccgagcgagt gcacggtgcg cccgtatttg aggtcgccga tcatcgcgat gcgcaggcct
      601 tccaccggtc cgttgatgtt cagttccttc ttgatcgtgt acaggtcgag cagcgcctgc
      661 gacggatgtt ccgacggacc gtcgcccgca ttgatcaccg gcacgcggct cgcgcgtgaa
      721 aattcctcga cggatcctgc caccggatgc cgcatcgcga tgatgtcgct gtagccgctg
      781 atcacgcgcg cggtgtcgta cagcgattcg ccttttgaga tcgcgctggc cttgatgtcg
      841 gtggtctcgc gcacgtgacc gccgagcagg ttgaacgcgc agccgaaact gacgcgcgtg
      901 cgcgtggacg gttcgaagaa caggttgccg aggatcgcgc cgtcgagcac gcgcgtgcgc
      961 ttttcgcggc gggcataggg gcccatggct tcggcgacgc ggaacagttc ctccacatcc
     1021 cggctgtcga actggttgat ggagaggatg ttggcgccgg
//
LOCUS       DASPLT010001042         1158 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_36033, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001042 DASPLT010000000
VERSION     DASPLT010001042.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1158)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1158)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1158
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_36033"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..339)
                     /locus_tag="VEA63_10820"
     CDS             complement(<1..339)
                     /locus_tag="VEA63_10820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HYD84540.1"
                     /translation="MSEHIGHECGIAMVRLLKPLAYFQEKYGTPLWGFEKLFLLMEKQ
                     HNRGQDGAGVACVKLDMPPGEPYMFRERSTMPSPLDRVFGAVLGRYHELVSAGTVHPE
                     FADTVKRHFDF"
     gene            543..>1158
                     /locus_tag="VEA63_10825"
     CDS             543..>1158
                     /locus_tag="VEA63_10825"
                     /inference="COORDINATES: protein motif:HMM:TIGR01444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HYD84541.1"
                     /translation="MKMSWHKKLSRQIRSRLRKKVLARAGIGVCADTDNGFFIVDPGD
                     FVVSRSLLERGEYDRREIEWLQRVVGPAPSTIVIVGVHVGAVLVPLAKRAARVIGFEP
                     NPPTYKLARLNLLLNEIGNTTLEHLAIGAGSGTVSIVNNPINTGNASISITTTAGSTT
                     IPLTSLDLYCPAKGIDSIDLLIIDIEGHELHALRGADQTLQRTAN"
BASE COUNT          230 a          382 c          328 g          218 t
ORIGIN      
        1 gaaatcgaag tggcgcttca ccgtgtccgc gaactccgga tgcaccgtgc ccgcgctcac
       61 gagctcgtga tagcgcccga gcaccgcacc gaacacgcga tcgagcggac tgggcatcgt
      121 gctgcgctcg cgaaacatgt agggctcgcc cggcggcata tcgagcttca cgcaggccac
      181 gcccgcgccg tcctgcccgc gattgtgctg cttctccatg agaagaaaca gtttttcgaa
      241 cccccagagc ggcgtgccgt atttctcctg aaaataggcg agcggcttca acagccgcac
      301 catggcaatt ccgcactcgt ggccgatgtg ctcgctcatg tttgtgccgt cgcgagtggc
      361 tggcgactcg ctcctcgatg cccgctgctt gggtgaatca tgtgcgcgtt aaaaggtccg
      421 atttcggcca cgtgtcgaat ttggtcaacg gctttctcgg acaggcacgt ttcaacgatc
      481 aagacggcgg cccaaagcgg cgttgcactc gcatggccgt aaactatcgg atgccggcac
      541 gcatgaaaat gtcctggcac aagaagctgt cgcggcaaat tcgcagtcgg ctgcggaaaa
      601 aggtgctggc gcgggccggc atcggcgtat gcgccgacac tgacaacggt ttcttcattg
      661 tggatccagg cgacttcgtc gtttcccgca gcctcttgga gcgcggcgaa tacgatcgcc
      721 gcgaaattga atggctgcag agagtcgtcg gcccggctcc gtcgacaatc gtgatcgtgg
      781 gcgtgcatgt cggagctgtt ctcgtcccgc tcgcgaaacg cgccgcccgc gtgatcggtt
      841 tcgagcccaa tccgccgacc tacaaactgg cgcgactcaa cctgctcctg aacgaaatcg
      901 ggaacacgac gctcgagcat ctggccatcg gcgccggtag cggaaccgtt tccatcgtca
      961 acaaccccat caacaccggc aacgccagca tctcgatcac gacgactgcc ggcagcacca
     1021 cgatcccgct cacctcgctc gatctgtatt gtcctgccaa aggcatcgat tccatcgacc
     1081 tactcatcat cgacatcgag ggtcacgaac ttcacgcgct gcgcggcgcc gatcagacgc
     1141 tgcaacgcac cgccaacc
//
LOCUS       DASPLT010001043         1068 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_216520, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001043 DASPLT010000000
VERSION     DASPLT010001043.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1068)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1068)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1068
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_216520"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1068
                     /locus_tag="VEA63_10830"
     CDS             <1..>1068
                     /locus_tag="VEA63_10830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007362162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycerophosphoryl diester phosphodiesterase"
                     /protein_id="HYD84542.1"
                     /translation="RRFTDEWGHIVEPVAVTYYTMLDVGNILLFTPEDQELRDRLKLG
                     AERLLEWQRPDGSWEVAYDHTTQKPLYTETPDLRPTFYGLLVAYRILGDEKYLEAARR
                     GADWVVENAVEKGRFLGVCGDTRFAPDFATAQLAQALLDLHAITGEVRYREAAIATAR
                     YYVTSIVTHPLPTRAEKFVNGKPRADWEITQAGLGFEHGGVIGSTWRRGPILLATHAG
                     LFVRVHALTGEPLFRNLARAAALARDAFVNPKTSVASYYWSNMDDGAGRFPHHAWWQI
                     GWITDYLISEATMRSAGQISFPAGFITPKVGPHRSYGFAPGQIFGDEAELAWGGVETD
                     HPAVDALVARATKARRVFVIL"
BASE COUNT          205 a          320 c          346 g          197 t
ORIGIN      
        1 gccgccgctt caccgacgaa tggggccaca tcgtcgagcc ggtcgccgtc acttattata
       61 cgatgctcga tgtcggaaac atcctactgt tcacgccgga ggatcaggaa ctgcgggatc
      121 gactgaagct cggcgcggag cgtctgctcg aatggcaacg cccggacgga agctgggaag
      181 tcgcctacga tcacaccacg caaaagccgc tttatacaga aaccccagac ctgcgtccga
      241 ccttttacgg tttgctggtc gcctaccgaa tactgggcga cgaaaaatat ttggaggccg
      301 cgcgacgcgg cgccgactgg gtggttgaaa acgccgtgga gaagggacgc tttctcgggg
      361 tgtgcggcga cacgcgcttt gcccccgact tcgccacggc gcaacttgca caggccctgc
      421 tcgatttgca cgcaatcacc ggcgaggtgc gctatcgcga agcggccatt gcgaccgcgc
      481 ggtattatgt gacctcgatc gtcactcacc cgctgccgac gcgcgcggag aagttcgtaa
      541 acggaaaacc acgcgcggat tgggaaatca cgcaggccgg attgggcttc gagcatggcg
      601 gcgtcattgg gtcgacgtgg cgacgcggtc cgatcctgct cgcgacgcat gcgggattgt
      661 ttgtgcgcgt gcacgcgctc acgggcgaac cgttgtttcg gaacctggcc cgcgcggcgg
      721 cgctcgcgcg cgatgcgttc gtcaatccga agacgagtgt cgcttcatat tactggagca
      781 acatggacga cggagccgga cggtttcctc accacgcctg gtggcaaatt ggatggatta
      841 ccgactatct gatttcagag gcgacgatgc gctcggccgg gcagatttca tttcctgcag
      901 gattcatcac accgaaggtc gggccacatc gcagctacgg attcgcgccg ggccagatct
      961 tcggcgacga agcggagctg gcctggggtg gagttgagac cgatcacccc gcggtcgacg
     1021 cgctggtcgc gcgagcaacg aaagcgcggc gggtgttcgt gatcctgc
//
LOCUS       DASPLT010001044         1552 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_299398, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001044 DASPLT010000000
VERSION     DASPLT010001044.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1552)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1552)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1552
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_299398"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            486..1298
                     /locus_tag="VEA63_10835"
     CDS             486..1298
                     /locus_tag="VEA63_10835"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HYD84543.1"
                     /translation="MKLPKLLSFAALSLALALSAAAKPQLGLQSWTCRNMTFEETVLF
                     AKAHGITQVEFFGKHLDPKSSKDENLAKLAFLKKHGVTAYSIGVSATTMNSDENRSLF
                     ELAKLFGMKVIVVEPGEPAQWDNLEALVKEYDIKLAIHNHGTGTVYGNPATVKHVLAE
                     RDHRIGVCMDVGWVTAAGFDAENIFRNYGDRVFDMHLKDKRLDKTDDRNRATDTFIGL
                     GNSNYAGLFAAIKETKWSGVMAIETDSADFAKDPAAFVAAAKKFFERHVGKN"
BASE COUNT          309 a          586 c          395 g          262 t
ORIGIN      
        1 gacctcgatg cgcgtcccgc acgaacagat cggctcggcc gccgtcacgc gtttggtgaa
       61 ccggatccaa caccccgccg cacctcgccg caaaatcctc gtcgaagccg aatacgtcgc
      121 cggcgaaacg atcacggcgc ccacgtcggc gtcggcctga ggcgacgcaa cgcgcgggcg
      181 ttcgccgccc cagaccacct cgaccgtagc gaaacgcgcg agcgttgcgt gcccgccttc
      241 gcgcagccgg ttgtagaagc ctgcttgcag gcgatccaac gcggcgcccg cgttcgacct
      301 gcgagatcgc ccgcaatcag gctcctacct cccctgcaga aaacgctgcg atttgtttta
      361 ccgctccacc gcccaccgcc cgccgcccac cgtcaactgt ccacggtcta ccgtccaccg
      421 tcgcgcgtcc accgcgctcg cgccgcttgc gcccaacgtc tttcctcctc caaatcgctc
      481 ctctcatgaa actccccaaa ctcctctcgt tcgccgccct ctcccttgcg ctcgctctct
      541 ccgccgcagc caaaccccag ctcggcctcc aatcctggac ctgtcgcaac atgacgttcg
      601 aggaaaccgt tctcttcgcg aaagcccacg gcatcacgca ggtcgagttc ttcggcaagc
      661 acctcgatcc gaaatcatcg aaggacgaaa atctcgcgaa actcgccttc cttaaaaaac
      721 acggcgtcac cgcttactcc atcggcgtca gcgccaccac gatgaactcc gacgagaacc
      781 gctcgctctt cgaactcgcg aagctcttcg gcatgaaagt catcgtcgtc gaacccggcg
      841 aacccgcgca gtgggacaac ctcgaggcgc tcgtgaagga atacgacatc aagctcgcca
      901 tccacaatca cggcaccggc accgtctacg gcaaccccgc caccgtaaaa cacgtcctcg
      961 ccgagcgcga tcaccgcatc ggcgtgtgca tggatgtcgg ctgggtgacc gccgctggct
     1021 tcgacgccga gaatattttc cggaattacg gcgaccgcgt gttcgacatg cacctcaagg
     1081 acaaacgcct cgacaaaacc gacgaccgta accgcgccac ggataccttc atcggtctcg
     1141 gcaacagcaa ctacgcaggc ctgttcgccg cgatcaaaga aaccaaatgg tccggcgtga
     1201 tggcgatcga gacggacagc gccgatttcg caaaagaccc cgccgccttc gtcgccgccg
     1261 cgaagaaatt tttcgaacgc cacgtcggca aaaactaagc accctcgtag cggcaggcct
     1321 ccatgcctgc ctgctccccg ctccgcacgt cagcgcgccc gggatatatc acgttccctc
     1381 cgcggaaatg tggctcgctc cgtgaggagc gggatgatcg aaccttggag cctcgcgcgt
     1441 gagcgcgagg caactgatct tccccgcgcc ctccgcttct tgtcgtagga gcctgctcgt
     1501 agccgatctt gaccgtccgc acctcgatat tgcctgcaag cccctcggca gg
//
LOCUS       DASPLT010001045         1840 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_135903, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001045 DASPLT010000000
VERSION     DASPLT010001045.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1840)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1840)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1840
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_135903"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..993)
                     /locus_tag="VEA63_10840"
     CDS             complement(<1..993)
                     /locus_tag="VEA63_10840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018287644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-3-phosphate synthase"
                     /protein_id="HYD84544.1"
                     /translation="MLASSKPIRAGINGSAAAARKVRVAVIGVGNCASALVQGVHFYR
                     NASAEEIVPGLMHVNLGGYHVRDIEFTAAFDIDATKVGRDLSEAIFSGPNNTVKFADV
                     PPSGVTVQRGMTHDGIGKYLAEKVQKAPGETADIVSILKRTKTDVVVSYLPVGSEMAT
                     KWYVEQVLEAGCGFVNCIPVFIASQPYWRKRFEERGLPLVGDDIKSQVGATITHRALV
                     NLFRERGVRLDRTYQLNFGGNTDFLNMLERERLDSKKISKTNAVTSQLGEPMPADQVH
                     VGPSDYVPWLTDQKWCYIHLHGTTFGNVPLKCEVKLEVWDSPNSAGVVIDAVRCA"
     gene            complement(1011..>1840)
                     /locus_tag="VEA63_10845"
     CDS             complement(1011..>1840)
                     /locus_tag="VEA63_10845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD84545.1"
                     /translation="NPRLTRVLGGISPIDPAFVQRMGRLGALDRLDVVAVHGFPLDWN
                     NWRLNDWPAKIAEIAAVTRLPVWVSEVGVSTFGADEVQVFGLDRTAELLVGRVPRIHW
                     YSLYDLPQAWPATTRHKEAEGSSYYRHFHMGLLREDGTPKPALAAFARHAPAMGLCQW
                     FHFEDHRLDEAVAWFRRLGVRHVRTGLSWADSFRPNADAWFDRVMGALEEFSVTVTFC
                     FTPEHLGVEPHHTSPPRELQGYAEFCARMIRRYAPAKADGAPTRERPAAKEFIATGI"
BASE COUNT          350 a          692 c          471 g          327 t
ORIGIN      
        1 ggcgcagcgc accgcgtcga tcaccacgcc cgccgaattc ggcgaatccc acacttcaag
       61 cttcacttcg cacttcaacg gcacgtttcc aaacgtcgtc ccgtgcaggt gaatgtagca
      121 ccacttctgg tccgtcagcc atggcacgta gtcgctcggc cccacgtgca cctggtccgc
      181 cggcatcggc tcgccgagct gactcgtcac cgcatttgtc ttcgaaatct ttttcgaatc
      241 caaccgctcg cgctcgagca tgttcaaaaa atccgtgttg ccgccgaagt tcaattgata
      301 cgtccggtcc aaccgcacgc cgcgctcgcg aaacaaattc accagcgcgc gatgcgtgat
      361 cgtcgccccg acctgcgact tgatgtcgtc gccgaccaac gggagtccgc gctcctcgaa
      421 tcgcttccgc caatacggct gcgacgcgat gaacaccgga atgcagttca cgaacccgca
      481 ccccgcctcg agcacctgct cgacatacca cttcgtcgcc atctccgaac ccaccggcag
      541 atacgacacc acgacatcgg tcttcgtgcg tttcaaaatg ctcacgatgt ccgccgtctc
      601 acccggcgcc ttctgaactt tctccgcgag atatttgcct atgccgtcgt gcgtcatgcc
      661 gcgttgaacc gtcacgccgc tcggcggcac gtccgcgaat ttcaccgtgt tgttcggccc
      721 cgaaaaaatc gcctcgctca aatcgcggcc gactttcgtc gcgtcgatgt cgaacgccgc
      781 cgtgaactcg atgtcgcgca cgtgatagcc gcccaagttg acatgcatca gccccggcac
      841 gatctcctcg gccgacgcgt tgcgataaaa gtgcacgcct tgcaccagcg ccgaggcaca
      901 gttgccgacg ccgatgaccg ccacgcgcac tttgcgcgcg gccgccgcgc tgccgttgat
      961 cccggcccga atgggtttcg atgacgctaa catgcgatgt cctccaattt tcagattccc
     1021 gttgcgataa attctttcgc cgccggccgc tctcgtgtcg gagcaccgtc cgctttcgcc
     1081 ggcgcatacc gccggatcat ccgcgcgcaa aactccgcat agccctgcaa ttcgcgcggt
     1141 ggactcgtgt gatgcggctc caccccgaga tgctccggcg tgaaacaaaa cgtcaccgtc
     1201 accgaaaact cctcgagcgc gcccatcacc cgatcgaacc atgcgtccgc attcggccga
     1261 aaactatccg cccagctcaa tcccgtgcgc acatgccgca cgccgagccg gcgaaaccac
     1321 gccaccgctt cgtccagccg gtgatcctcg aaatgaaacc actgacacag ccccatcgcc
     1381 ggcgcatgcc gcgcaaacgc cgcgagcgcc ggcttcggcg ttccgtcttc acgcagcagc
     1441 cccatgtgaa aatgccggta atacgacgag ccctccgcct ccttgtgccg cgtcgtcgcc
     1501 ggccacgcct gcggcaaatc gtaaaggcta taccaatgga tccgcggcac gcgcccgacg
     1561 agcagctccg ccgtgcgatc gagcccgaac acctgcactt cgtccgcacc gaacgtcgac
     1621 acgccgacct ctgacaccca caccggcagc cgcgtcaccg ccgcgatctc cgcgatcttc
     1681 gccggccagt cgttgagccg ccagttgttc caatccagcg gaaacccgtg caccgcgacg
     1741 acgtcgagcc ggtcgagcgc gcccaaccga cccatccgct gcacgaacgc gggatcgatt
     1801 ggcgaaattc cgccgagcac gcgcgtcagc cgtggattct
//
LOCUS       DASPLT010001046         1172 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_299463, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001046 DASPLT010000000
VERSION     DASPLT010001046.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1172)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1172)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1172
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_299463"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            229..>1172
                     /locus_tag="VEA63_10850"
     CDS             229..>1172
                     /locus_tag="VEA63_10850"
                     /inference="COORDINATES: protein motif:HMM:NF015684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HYD84546.1"
                     /translation="MNLLDRYIFKSVLFTCTAAVTMFAFIVLVPNIAKDLMAYVLAGQ
                     IPAATAAKLILLLIPHAVTYALPMGMLTGVLLTLGRLSADSEITAMRAAGIGIARATR
                     PVVILAILAVGIGLYFNFDSMPRTRVEYYRSLAGAIRANPLSLIVPKTFIRDFSGYVV
                     YVGDKQGGLLRDIWIWELDAERRVTRFAHAESGRIDYNEDHNAIIFTPLHVQVETRDP
                     KNPERLVEPQLVVSVEQWEPLHFSLDGVFGRAGSVRYKQEWMTYDQIQAERARLAALP
                     NPTDPAAAHQLARDRMKIDVIFSDKFNTALAVLSLTFMG"
BASE COUNT          201 a          432 c          308 g          231 t
ORIGIN      
        1 tcacgcgttt cgctacagca gactacgcgc acctcgcatc tgcgtgtgtt gaatgcgggc
       61 taaatgtagg gccgggtttc ccgaccccgc cgctttgctg ctccggcggg ttcaggagaa
      121 cccgccctac aacaacaccg ccgctgttat ctctcctcgc gcccgcatcc cacgaagcgc
      181 cttggcagcg cgcctcgcgt gtgcgacgaa tgccgctcgc tgctccgttt gaacctcctc
      241 gaccgttata ttttcaagag cgtcctgttc acctgcaccg ctgcggtgac gatgttcgcg
      301 ttcatcgtcc tcgtgccgaa catcgcgaag gacctgatgg cctacgtcct cgccggccag
      361 attcccgccg cgaccgccgc caagctcatc ctcctgctca ttccgcacgc cgtcacttac
      421 gcgctgccga tgggcatgct cacaggcgtg ttactgacgc tcggccgttt gtccgcggat
      481 agcgaaatca ccgcgatgcg tgccgccggc atcggcatcg cccgcgctac gcgcccggtc
      541 gttatcctcg cgattctcgc ggtcggcatc ggcctctact tcaacttcga ttcgatgccg
      601 cgcacgcgcg tcgagtatta ccggtcgctg gccggcgcga tccgcgcgaa cccgctcagc
      661 ctgatcgtcc cgaaaacctt catccgtgat ttctccggct acgttgtcta cgtcggcgac
      721 aaacaaggcg gcctcctccg cgatatctgg atttgggaac tcgacgccga acgccgcgtc
      781 acgcgcttcg cccacgccga atccggtcgc atcgactaca acgaggatca caacgccatc
      841 atcttcacgc cgctgcacgt gcaggtggaa acgcgcgacc cgaaaaatcc cgagcgcctc
      901 gtcgagccgc agctcgtcgt ctccgtcgag caatgggaac cgctgcattt ctccctcgat
      961 ggcgtcttcg ggcgcgccgg cagcgttcgt tacaaacagg aatggatgac ctacgatcaa
     1021 atccaagccg aacgcgcccg cctcgccgct ctgcccaatc ccaccgatcc cgccgccgcg
     1081 caccaactcg cgcgcgatcg gatgaagatc gacgtcatct tcagcgataa attcaacacc
     1141 gcgctcgccg tcctctcgct cacgttcatg gg
//
LOCUS       DASPLT010001047         1117 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_36117, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001047 DASPLT010000000
VERSION     DASPLT010001047.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1117)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1117)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1117
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_36117"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..546
                     /locus_tag="VEA63_10855"
     CDS             <1..546
                     /locus_tag="VEA63_10855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HYD84547.1"
                     /translation="ALGQKFVEFVETQMAFYASPAGDMKLWFGGDERVGDVWDRSVGA
                     AYRFQKETNGKLVPEMSPWRFNVATNYLFDRGILKGASLGGAFRWQDGQILGYALKPD
                     FSNLDVNKPYWGESESALDLWVGYERKLTQRIRWRIQLNLRNVGDSVSLVPISVQPDG
                     TPALQRIREGQTWTLTNTFSF"
     gene            678..>1117
                     /locus_tag="VEA63_10860"
     CDS             678..>1117
                     /locus_tag="VEA63_10860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD84548.1"
                     /translation="MKIFPFRAFVAGSLLLATAAVAQAAAAGSTGTGLLSGDWTAERL
                     KAVLRAPADWVPFPTAAQRSRWEALPAEVRAACVLRAERVMHDWPELTATDALDFQRT
                     GRRTAFQTKYFSRRAHLQALVLAECVEGEGRFADVIADRVWSLC"
BASE COUNT          189 a          332 c          371 g          225 t
ORIGIN      
        1 gcgctcggcc agaagttcgt ggagtttgtc gagacgcaga tggctttcta cgcgtcgcca
       61 gccggcgaca tgaagctctg gttcggcgga gacgaacggg tcggcgacgt ttgggatcgc
      121 agcgtgggcg cggcgtatcg attccagaaa gagaccaacg ggaaactcgt tcccgaaatg
      181 tcgccgtggc ggttcaatgt tgcgaccaac tacctcttcg accgaggcat cctgaaaggc
      241 gcgagcctcg gcggcgcctt ccgctggcag gatggccaga tcctcggcta cgcgttgaaa
      301 cccgacttct cgaatctcga cgtcaacaaa ccctactggg gtgagtcgga gagtgcgctg
      361 gatctgtggg tcgggtatga acgcaagctc acgcagcgga tccgttggcg gattcagctc
      421 aacctgcgca acgtcggcga ctcggttagc ctcgtgccga tcagcgtcca gcccgacggc
      481 acgccggcgc tgcaacgtat ccgcgaaggc cagacatgga ctttgacgaa cacgttttcg
      541 ttctaagtcg tttttttccc atcggcccgc ttctcgcgga gcgggccgtg cggtttccgt
      601 agccctgcgt gtgaacgcag ggatttcgca ccgagcaaag gactttgaaa acacgcctta
      661 gattcgccag acgactgatg aaaatctttc cctttcgcgc gttcgtggcc ggttcgctcc
      721 tgttggccac cgcagccgtg gctcaggccg ccgccgcggg atcgacgggg acgggtttgc
      781 tttcgggcga ctggaccgcg gagcgactga aggcggtgct gcgcgcgccc gcggactggg
      841 tgccgttccc gactgcggcg cagcgatcgc gttgggaagc gcttccggcg gaggttcgcg
      901 ctgcgtgtgt tctgcgcgcg gagcgtgtga tgcacgactg gccggaactc accgcgacgg
      961 atgcgctgga tttccaacgc accggtcgtc ggaccgcgtt tcaaacgaag tatttctcac
     1021 ggcgcgcgca tctgcaggcg ctcgtgctgg ccgaatgtgt cgaaggcgaa ggacgttttg
     1081 ccgacgtcat tgcggatcgc gtctggtcgc tttgcga
//
LOCUS       DASPLT010001048         2475 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_72633, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001048 DASPLT010000000
VERSION     DASPLT010001048.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 2475)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2475)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2475
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_72633"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(38..1195)
                     /locus_tag="VEA63_10865"
     CDS             complement(38..1195)
                     /locus_tag="VEA63_10865"
                     /inference="COORDINATES: protein motif:HMM:NF017077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE3 domain-containing protein"
                     /protein_id="HYD84549.1"
                     /translation="MKDKTLRRVFVFFVVIFALVALVAAYAVRSLNRAVASNDWVNHT
                     HAIIAEIDGAFSTLRLGEGAGRAHVFTGAPRDLAAARSAFDVMEEHFEIAKALTRNDA
                     ATHERVLRLETLSRARRDLAEQVAEARAADLTENVQALLAADAAVDAIGEIGRAVDTL
                     KETQMALLAARDRASYRQVQTTRWIVSTGVALNLVLFGGVAWLIRDDLAARTRLAATL
                     QEANARLETKVRERTSELAAINGRLVAENLERRWSTQAIEHQLRYNQAIVDAVGDLVF
                     VMTKALNITRLNPAVVHATGWEPQELLGQPIEKIFRWSSGARDGAEANAPMTQALREG
                     RDLKNEWAELSDRRGGNIAARATLFPLRDRDKVVGGILILQLGAKPGGARA"
     gene            complement(1229..1723)
                     /locus_tag="VEA63_10870"
     CDS             complement(1229..1723)
                     /locus_tag="VEA63_10870"
                     /inference="COORDINATES: protein motif:HMM:NF016364.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HYD84550.1"
                     /translation="MASLGAVTLDGLLNDPKLTPKRFASVFEHFAYEYHPAVQSPERF
                     LEARVGDCDDYAILADHVLKQKGFTTRLVHVRMVGMVAHAVCYVVQSKAYLDYNNRIY
                     SVNLERSGASLRQIAEKVSASLEVNWSSATEFTYDYGAARKQMGMTVVKVDSPSQDPD
                     RPPS"
     gene            2212..>2475
                     /locus_tag="VEA63_10875"
     CDS             2212..>2475
                     /locus_tag="VEA63_10875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD84551.1"
                     /translation="MSRYPAIFQQHRGLVWSIAAPLSLAISTVVVAALVINSLFLSPY
                     RLMAERGIVHITSGERSGLLSFPDLVDVQRKNSSFQQVFAASSP"
BASE COUNT          456 a          866 c          681 g          472 t
ORIGIN      
        1 taaagtccgt tgtaggtccg atggcgtgag cccgcgttta cgcgcgagcg ccgccgggct
       61 tcgccccgag ctgcaaaatc aggattccgc ccaccacctt gtcgcgatcg cgcaaaggaa
      121 agagcgtcgc gcgcgccgcg atgttgccgc cgcgacgatc gctcagctcc gcccactcgt
      181 tcttcaaatc gcgtccttcc cgcagcgcct gcgtcatcgg tgcgttggcc tccgcaccgt
      241 cgcgcgcgcc gctcgaccaa cggaaaattt tctcgatcgg ctgaccgagg agttcttgcg
      301 gctcccaacc ggtcgcgtga accaccgccg gattgagccg cgtgatgttc aacgccttcg
      361 tcatcacgaa caccaaatcg ccgaccgcat cgacaatcgc ctggttgtaa cgcagctgat
      421 gctcgatcgc ctgcgtcgac cagcggcgtt ccagattttc ggcaacgagg cgtccgttga
      481 tcgccgccag ctcgctcgtc cgttcgcgca ccttcgtctc gagccgcgcg ttcgcctcct
      541 gcaacgtcgc ggccagccgc gtgcgcgccg ccagatcgtc gcgaatcaac cacgccacgc
      601 cgccgaaaag aaccaagttg agcgcgacac cggtcgagac gatccagcgg gtcgtctgca
      661 cctgccgata ggacgcgcga tcgcgtgcgg cgagcagcgc catctgggtt tccttcaacg
      721 tgtcgaccgc ccgcccgatc tcgccgatcg cgtcgacggc ggcatccgcg gcgaggagcg
      781 cctgcacgtt ttccgtcaga tcggcggcgc gcgcttccgc cacttgctcc gcgagatcgc
      841 ggcgcgcgcg gctcaacgtt tccaaccgca gcacccgctc gtgcgtcgcc gcgtcgttcc
      901 gcgtgagcgc cttcgcgatc tcgaaatgct cctccatcac atcgaacgcg ctgcgcgctg
      961 ccgcgagatc gcgcggggcc cccgtgaaca catgcgcccg ccccgctcct tcaccgagcc
     1021 gcagcgtcga aaacgccccg tcgatttccg cgatgatcgc gtgcgtgtgg ttcacccaat
     1081 cgttgctcgc caccgcgcga ttgaggctcc gcaccgcata agcagccacg agcgccacga
     1141 gggcgaagat cacgacaaag aaaacgaaaa cgcggcggag ggttttgtct ttcacgcgct
     1201 ggaaggaggc ggtgccaagg ggtggatttc agcttggagg gcgatcggga tcttgcgagg
     1261 gcgaatccac tttcaccacc gtcattccca tctgcttccg cgccgcgcca taatcgtagg
     1321 tgaactcggt ggccgacgac cagttcacct ccagcgaggc cgaaactttt tccgcgatct
     1381 gccgcaagct cgccccgctg cgctcgaggt tcaccgagta aatccggttg ttgtaatcga
     1441 gatacgcctt gctctggacc acatagcaaa ccgcgtgcgc caccatcccg accatccgaa
     1501 cgtgcacgag ccgggtcgtg aacccttttt gcttcaacac gtgatccgcc aaaatcgcgt
     1561 agtcgtcaca atcgccaacc cgcgcctcca aaaaacgctc cggcgactgc accgccggat
     1621 gatactcgta ggcgaaatgc tcgaagacac tcgcgaaacg cttcggcgtg agcttcgggt
     1681 cgttcaacaa cccgtccaac gtcacggcac ccagcgatgc caacccgcct ccaaatgcca
     1741 cgcctcccac gcagagctgg gcaagccgcc gccaccttac cttaagatgt agattcggca
     1801 cgcgatgcgt tgttgctctc ggaatgacat gcggccctcc gcacgaccac ttatttttgc
     1861 ggcctgctcg atcggcgcgc aacgagccgc gctagtaggg gtgtctcaca agaaacctgg
     1921 aaaaatccag tggcgcggca gcgccggtag ggactcctct ccgaggcgtc cgcgcacacg
     1981 ggaaacgcgg cgaatttcta agtaatctcc gggataccat actcggcaat ccatcgaacg
     2041 ctttcgccat cgcacgaatc ggcgagcttc gccctgcgcg cttcctgtgc ggcagcccgt
     2101 ctttttcgca cgtttccttt tgtagtctaa tgtgacagct aaccggctct gaataaacca
     2161 ttgccaagaa actgcgggag ctcgtagcta cacactcctt cttcaatctt gttgagccgt
     2221 taccccgcaa ttttccagca acaccgcgga ctcgtttggt ctatcgccgc ccccctctcg
     2281 cttgcgatct cgaccgtcgt cgtcgccgcc ttggtgatca attcgctgtt tctcagcccg
     2341 tatcggctga tggccgagcg cggtatcgtg catatcacca gcggcgaacg cagcgggttg
     2401 ttgagttttc ccgacctcgt cgacgttcaa cggaaaaaca gctccttcca acaggtcttc
     2461 gccgcgagca gcccc
//
LOCUS       DASPLT010001049         1173 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_72643, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001049 DASPLT010000000
VERSION     DASPLT010001049.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1173)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1173)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1173
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_72643"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..802
                     /locus_tag="VEA63_10880"
     CDS             <1..802
                     /locus_tag="VEA63_10880"
                     /inference="COORDINATES: protein motif:HMM:NF014841.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HYD84552.1"
                     /translation="GLLAELPNLRAVFYAAGTIKDIVTAASWDRGIRITTAAHENAKP
                     TAEFAFAQIILSLKRVWDRMFLLREQRQFIQRDPLARGCYGATVGLLALGKIGRLVAA
                     RLQTLDVHVIAYDPFVDSAEAARLGVRLCPIEEVFATADVVSCHLPATPKTEQMLGRT
                     LFAQMKRGATFINTARGRVVDEPGLVAVLRERADLYAVLDVTVHEPPLPEDPLFSLRN
                     VVLTPHIAGSIADECRRMGRMMVDEIERYLDDRPLRGEVFREQLEMMA"
     gene            927..>1173
                     /locus_tag="VEA63_10885"
     CDS             927..>1173
                     /locus_tag="VEA63_10885"
                     /inference="COORDINATES: protein motif:HMM:NF015000.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HYD84553.1"
                     /translation="MKIRLVFILILLVGRFANASVDTAYKLFAHRGGVVEDKFPDNSA
                     DAVRAAIERGYWGLEIDIRETKDGVLVLRHDADLKLYY"
BASE COUNT          207 a          411 c          336 g          219 t
ORIGIN      
        1 cgggctgctc gcggagttgc ccaaccttcg cgcggtcttc tacgccgccg gcaccatcaa
       61 ggacatcgtc accgccgcgt cgtgggatcg aggcattcgc atcaccacgg ccgcgcacga
      121 gaatgcgaaa cccaccgccg agttcgcctt cgcccaaatc atcctctcgc tgaaacgcgt
      181 ctgggaccgc atgttcctcc tccgcgaaca gcgccagttc atccagcgcg atcctctcgc
      241 ccgcggttgc tacggcgcca ccgtcggact gctcgccttg ggcaaaatcg gccggctcgt
      301 cgccgcgcgc ctgcaaaccc tggacgtcca cgtcatcgct tacgatccgt tcgttgattc
      361 cgccgaagcc gcgcggctcg gcgtgcgcct ctgcccgatc gaggaggttt ttgccacggc
      421 tgacgtcgtc tcctgtcacc tgcccgccac gccaaagact gagcagatgc tcggccggac
      481 gctcttcgcg cagatgaagc gaggtgcgac gttcatcaac accgcgcgcg gtcgcgtcgt
      541 cgacgaaccc ggtctcgtcg ccgtgctgcg cgagcgagcc gatctctacg cagtgcttga
      601 tgtcacggtg cacgagccgc cgctgcccga agacccgctc ttcagcttgc gcaatgtcgt
      661 gcttacgccg cacatcgccg gcagcatcgc cgatgaatgt cgccgcatgg gtcgcatgat
      721 ggtggacgaa atcgaacgct acctcgacga ccgcccactc cgcggcgaag tgttccgcga
      781 gcagttggaa atgatggcct gaacgcgaga gcgctcgcgc cctcggagaa gggatcattt
      841 ccgtcgaacg cgcgaagata tcctgaagtt cccgcgaggt gactcgcttc gttctcacca
      901 cccagtttta gcggttccgc cacaccatga aaatccggct cgtcttcatc ctcatcctcc
      961 tcgtgggacg cttcgccaac gcatcggttg ataccgcata caaactcttc gcccatcgcg
     1021 gcggcgtggt ggaggacaag tttcccgata actccgcgga cgcggttcgc gcggcgatcg
     1081 agcgaggcta ctggggactc gagatcgaca tccgcgagac gaaagacggc gtgctcgtgc
     1141 ttcgtcacga cgccgatctc aaactctact acg
//
LOCUS       DASPLT010001050         1575 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_13086, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001050 DASPLT010000000
VERSION     DASPLT010001050.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1575)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1575)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1575
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_13086"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(511..1455)
                     /locus_tag="VEA63_10890"
     CDS             complement(511..1455)
                     /locus_tag="VEA63_10890"
                     /inference="COORDINATES: protein motif:HMM:NF013695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HYD84554.1"
                     /translation="MAEAVAPTPIQLSRERSLRFAMGVDALLIVVVSCIGLIGGSLTI
                     VAECVRGTLMLSTEIFALIAMRRIHRGQLAELEFGHGKLEQIANLAIAAGMVFGATWI
                     GWGAVDLAFGDRPPATPGGLAASAMIAALNTFLNFVAWLSVLQATPVGSPVIMEAQLK
                     SRSVKFFSSCIVLVLLTTSALCLDPVIATWCDVLGALFVAGFILVTAASMTRGGLPDL
                     LDQSANEAVQRSINRSLAQHFDDYDQLSRVRSRRSGKTIFIEVALGFDPRLTFAEVNR
                     RVNTIRATFLREIPDSDVSIVVSTLSRNLSGSDTAEAA"
BASE COUNT          324 a          439 c          565 g          247 t
ORIGIN      
        1 cttttcgcga cgctaccggc aggcgtatgg gcaggagaag acccggttgt agccggaaag
       61 ccggcagcgg ctcgctcgca gcgagcggcg ggccgcgcgg agataggtct gcgctttcca
      121 aggatcaatg accaagttta cccgcctttg gcggtgccga aggcgaccag gggtccagtg
      181 aaactcaaaa ggccaagctt caaaacgggt cacgctcggg atgtgagctg cgaatgccgg
      241 tgtcgtgcaa tgacggcgcg cgcaagcgcg gcgatgccga agagcgcggg ataggcggag
      301 ggctttggga acgcgcgggc aacggtggaa tgcgcgaact catgcagtaa aagtggagcc
      361 cgacgacgtc gtcttcatcc aaagcgtcct ccccgggttt gttttgtgat ggtagggcga
      421 gccgtctcag gcgagccgcg aatgttgaaa tgcgaatgca aggccggctc ggcggggacg
      481 cctcgcccta ccatcgagcc cgttcgaagc tcacgccgct tccgcggtgt ctgaaccgct
      541 gagattccgg ctgagggtgg agacgacaat ggagacgtcg gaatcgggga tctcgcggag
      601 aaatgtcgcg cggatcgtgt tcacgcggcg gttgacttca gcgaaggtga ggcgcgggtc
      661 gaaaccgagc gcgacttcga tgaagattgt cttgccggaa cgacgcgagc gcacgcggct
      721 gagttggtca tagtcgtcga agtgctgagc gagcgagcga ttgatgctgc gttggacggc
      781 ctcgttcgcg ctttgatcga ggaggtcggg caggccgccg cgggtcatgc tggcagcggt
      841 gacgaggatg aagccggcga caaacaacgc gccgagcaca tcgcaccacg tggcgatcac
      901 cggatcgaga cagagtgcgg aggtcgtgag caacacgagc acgatgcacg acgagaaaaa
      961 tttcaccgag cgcgatttga gctgcgcttc catgatcacg ggcgagccga cgggcgtggc
     1021 ttgcagcacg gagagccagg cgacgaaatt gagaaacgtg ttcagggcgg cgatcatggc
     1081 cgaggcggcg aggccgccgg gcgtggcggg cgggcggtcg ccgaaagcga ggtcgaccgc
     1141 accccagccg atccacgtgg cgccgaacac catgccggcg gcgatggcga ggttcgcgat
     1201 ttgttcgagt ttgccgtgac cgaattccag ttcggcgagt tgcccgcgat gaatgcgacg
     1261 catcgcgatg agcgcgaaga tttccgtgct cagcatcagc gtgccgcgaa cgcattcggc
     1321 gacgatggtg agcgagccgc cgatgagtcc gatgcaggaa accactacga tcagcagcgc
     1381 gtcgacgccc atcgcgaagc gaagcgagcg ttcgcgcgag agctgaatgg gtgtgggcgc
     1441 gacggcttca gccatggctc acaagcgcac gcgagctcgc atgatactga aaatactcac
     1501 gtcgggaaaa tccacatacg cggcgcgacc gacgggcgag cggatgcggt ccgcttccag
     1561 cggttcgagc cgcac
//
LOCUS       DASPLT010001051         1302 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_216670, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001051 DASPLT010000000
VERSION     DASPLT010001051.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1302)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1302)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1302
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_216670"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(22..>1302)
                     /locus_tag="VEA63_10895"
     CDS             complement(22..>1302)
                     /locus_tag="VEA63_10895"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HYD84555.1"
                     /translation="GLLGIALHPNFERNHWVYLFWTESSTGADTDQVADVGNPNSAFP
                     PGTPQPFGHRVDRFVWDRRAQKLKFDRNLIVLRAYQADAGQPLRGNHNGGVLRFEKDD
                     EHHFGRLADVFGFGQRDQRHAKLFIVIGDSGRRGMLQNLLQGPFGNDTPDDEFGGPEP
                     DNAHLTGVILRLNDDGSTPRDNPFYDYGATLGGEEGANLQRIYAYGIRNSFGLAVDPW
                     TGELWESENCDDAFDEINRIEPGHNGGWIQAMGPLSRVAEFKQIETTFPTPPGALQQV
                     RWPPSNIADDPISARARMVDFPGSHYADPQFSWKWALPPAAIGFMTDKSLGKEHEGAL
                     FVGAALPFNAGGFLFRFELTPNRQRIAWTDPKLADLVADNTAKYDFAESESVLFGQNF
                     GSGVDILTGPKGNLYVVSASKGAVFEIHRPRGSR"
BASE COUNT          230 a          365 c          452 g          255 t
ORIGIN      
        1 cccctcgcgc gccggcgttt tttatcggct gccgcggggg cgatgaatct cgaacacggc
       61 gcccttcgag gcggagacca cgtagagatt tccttttggc ccggtcagga tgtcgacacc
      121 gctgccgaag ttttggccga agagcaccga ttcgctttcg gcgaaatcgt atttggcggt
      181 gttgtcggcc acaaggtcgg cgagctttgg atcggtccac gcgatgcgct ggcgattcgg
      241 cgtgagctcg aaacgaaaca gaaagccacc ggcgttgaac ggcagcgcgg ctccgacgaa
      301 gagcgcgcct tcgtgttcct tgccgagcga cttgtcggtc atgaaaccga tggcggcggg
      361 cgggagcgcc catttccaac tgaactgcgg atcggcgtag tgcgaaccgg gaaagtcgac
      421 catgcgagcg cgcgcggaga tcggatcgtc ggcgatgttg ctgggcggcc agcgaacttg
      481 ctgcaacgcg ccgggtggcg tggggaacgt cgtctcgatt tgtttgaact cggcgacgcg
      541 cgagagcggg cccatcgctt gaatccagcc gccattatgg ccgggttcga tgcggttgat
      601 ctcgtcgaaa gcgtcgtcgc aattttcgga ttcccacagc tcgccggtcc acggatcgac
      661 cgcgaggccg aagctgttgc ggatcccata cgcgtagatg cgttgtagat tcgcgccttc
      721 ctcgccgccg agcgtggcgc cgtagtcgta gaaaggattg tcgcgcggcg tggaaccgtc
      781 gtcgttgagg cggagaatca cgccggtgag atgggcattg tcgggctcgg gcccgccaaa
      841 ctcgtcgtcg ggcgtgtcgt tgccgaacgg accttgcagc agattttgga gcatgccgcg
      901 acggccgctg tcgccgatca cgatgaagag tttcgcgtga cgttgatcgc gttgtccgaa
      961 accgaataca tcggcgagcc ggccgaagtg gtgctcgtcg tctttttcga aacggaggac
     1021 gccaccgttg tgattgccgc gaagcggttg gccggcgtcg gcttgatacg cgcggagcac
     1081 gatgagattg cgatcgaact tcagcttttg ggcgcggcgg tcccagacga aacgatcgac
     1141 gcgatgaccg aacggctggg gcgttcccgg cggaaacgct gagttgggat tgccgacgtc
     1201 ggcgacctgg tcggtgtcgg cgccggtgct gctctcggtc cagaaaagat aaacccagtg
     1261 attgcgctcg aagttgggat gaagcgcgat gccgaggaga cc
//
LOCUS       DASPLT010001052         3875 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_135993, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001052 DASPLT010000000
VERSION     DASPLT010001052.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 3875)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3875)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3875
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_135993"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            324..2399
                     /locus_tag="VEA63_10900"
     CDS             324..2399
                     /locus_tag="VEA63_10900"
                     /inference="COORDINATES: protein motif:HMM:NF015476.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009044 - xylan 1,4-beta-xylosidase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 52 protein"
                     /protein_id="HYD84556.1"
                     /translation="MAEESYHTQHSPFGAFASFTVGLVDSPGGFGQSLRGPAKQNVYV
                     GFRGPAHQEWQLLPFLTPPKSQEGAFTGDTTVVQPPRGFNALRPADYERTLGWASDTW
                     RADASRFGFSLLSPFDKVPDPAKMSKSTARFHLAPVVTGWIEYDNRAGKAPVELIFGI
                     GNGDDTLRPLTDTSKSLLGFAGGTAFGYATSPARGVAMRQGFDVLAPKFKDYRGLLVI
                     AGETALVFTVPAGQRKRFPLALGFYAAGTITTGLSSTFAYTRVFRDLEDVLTHGLTEH
                     ARYTKVAAQRDRELAKSRLDADQKFLIAQATHSYLGNSQLMWQKNQPLWIVNEGEYRM
                     INTFDLTVDHVFFELAWLPWAVRDTLDLFVDRYSYRDQLGLSFTHDMGVNNFFTPPGR
                     SSYECDNIEGCFSHMTMEQLLNWVLTATTYAAHTGDNAWLRANQKTLLACADSLHRRD
                     HPDASKRDGILKHDTSRCGEEGSEITTYDSLDVSLGQARNNLYLAVKTLGAWVLLENA
                     FATLKLPKASADARATADLLAQTLTTKFESDTGFFPAVFEKGNRSRILPAVEGFVYPL
                     FLGYKDAVDRNGRFAPLFKQLSQHMAQALQTGICLDEKSGGWKMSSTSTNTWFSKIAI
                     AQHVVRELFPEVMNDAARRGDRVHADWQRTPGCGEHAMCDQIRSDSGIVCGSRYYPRG
                     VSAYLWLSE"
     gene            2478..>3875
                     /locus_tag="VEA63_10905"
     CDS             2478..>3875
                     /locus_tag="VEA63_10905"
                     /inference="COORDINATES: protein motif:HMM:NF024742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HYD84557.1"
                     /translation="MSDSAHKIPVREKIGYALGDVATNFFFQSMILYQARFYTDTVGL
                     APAIVGWMFLIVRLGDAFVDPIVGALSDRTQSRWGKFRPWILWTALPFGLIFYFVYVA
                     PNFGMQGKIIYAVVTYSLMMVIYSANNTPYAALMGVMTPNVSERANIARYRFVAALIG
                     QFIIQALALPLVDKFGAGNSAKGWATTMAIFGVIIVVCNIIVFFTTKERVLPNPEQKP
                     SIKEDIKNTWGCKPWQAMFVLTLAIFTMLVIRGTSLNYLFTYYLDPAAMTRFVDTIGL
                     AAAATGGEPTGFAYVLDLFGLLVRPDNSNAPAVAFAFFQVLGSIIQIIAIPFSKPLSD
                     RFGKKAVFIAGMSVTILATLGVFLIGPTDMNLMFWLSIIWGLGWGPTVPLLWVMIADV
                     ADYSEWQTSRRATGFMYAGILFALKAGLGLGGAVIGWIMGLHGYVANVVQSESAMLGI
                     RLGSSVYAAVPMLIGL"
BASE COUNT          698 a         1364 c         1030 g          783 t
ORIGIN      
        1 caaagccagg gaaaccgtcg tgacgtgcac tccggcctgg cgcgcaacgt ctgcgagcgt
       61 aacgcgacgt tggttcatag tccgcccgca ttgcgaccga tgtcgcgcgc ggcgacaagt
      121 ctattgttag agtgtgcacc cgatggcgtt agccggacgc gaaaaagaca ttcccgcact
      181 ccgttgaagg gccgtcgcgg ctccagctta cgccccttgt atgattaacg cccgtcctcg
      241 ttttgcgttc cacttaacgc ttccgtccgg ctcgaactct gcttcgtttg cccacgcaaa
      301 gccccaagcc ccctactctt accgtggctg aagaaagtta ccacacgcag cattcgccct
      361 tcggtgcgtt tgcgagtttc accgtcggtc tcgtcgattc tcccggcggt ttcggccaat
      421 cgcttcgcgg ccccgcgaaa caaaacgtct acgtgggctt ccgcggcccc gctcaccagg
      481 agtggcaact actgccgttc ctcaccccgc cgaaatcgca ggaaggcgca ttcaccggcg
      541 acacgaccgt cgtccaaccg ccacgcggct tcaacgcgct ccgccccgcc gactacgaac
      601 gcacgctcgg ctgggcgagc gacacctggc gcgctgacgc ctcgcgcttc ggcttctccc
      661 tgctctcgcc tttcgacaag gttccggacc ccgcaaaaat gtcgaagtcg acggcgcgct
      721 tccatctcgc acccgtcgtt accggttgga tcgaatacga caaccgcgcc ggcaaagccc
      781 ccgtcgaact catcttcggc atcggcaacg gcgacgatac gcttcgcccg ctcaccgaca
      841 cgagcaagtc gctcctcggt ttcgccggcg gcaccgcttt cggctacgcc acttcgcccg
      901 cgcgcggcgt tgcgatgcgc cagggcttcg acgtgctcgc gccgaagttc aaagattacc
      961 gcggtctcct cgtcatcgct ggcgaaaccg cgctcgtctt caccgtccca gccggccagc
     1021 gcaaacgctt cccgctcgcc ctcgggttct atgcggcggg cacgatcacg acgggcctct
     1081 cgtcgacctt cgcctacacg cgcgttttcc gcgatctcga agacgtgctc acgcacggtc
     1141 tcaccgaaca cgcccgctac acgaaagtcg ctgcgcagcg cgaccgcgaa ctcgcgaaaa
     1201 gccgcctcga cgccgaccag aaattcctca tcgcgcaagc cacgcacagc tacctcggca
     1261 attcacagct catgtggcaa aagaaccagc cgctgtggat cgtcaacgag ggcgaatacc
     1321 gcatgatcaa cacgttcgat ctcaccgtcg accacgtgtt cttcgaactc gcctggctgc
     1381 cgtgggctgt ccgcgacacg ctcgatctct tcgtcgaccg ctactcgtat cgcgaccagc
     1441 tcggcttgtc gttcacccac gacatgggcg tgaacaattt cttcacgccg ccgggccgct
     1501 ccagctacga atgcgacaac atcgagggct gcttcagtca catgacgatg gagcagctcc
     1561 tgaactgggt gctcaccgcg acgacttacg ccgctcacac cggtgacaac gcctggctcc
     1621 gcgcgaacca gaaaacgctt ctcgcctgcg ccgacagcct tcaccgccgc gaccatcccg
     1681 acgcgtcgaa gcgcgacggt attctcaaac acgacacctc ccgctgcggc gaagaaggct
     1741 cggaaatcac gacctacgac agcctcgacg tcagcctcgg tcaggcgcgc aacaatcttt
     1801 atctcgccgt taaaaccctc ggcgcctggg tcctcctcga gaacgctttc gccacgctaa
     1861 aactcccgaa agcctccgcc gacgcccgcg cgaccgccga tctgctcgcc caaacgctca
     1921 cgacgaaatt cgagtccgac acgggcttct tccccgccgt gttcgagaag ggcaaccgct
     1981 ctcgcatcct ccccgccgtc gaaggcttcg tttacccgct cttccttggc tacaaggacg
     2041 ccgtcgatcg caacggtcgc ttcgctccgc tcttcaaaca actgagccag cacatggcgc
     2101 aggccctgca aaccggcatc tgcctcgatg aaaaaagcgg cggttggaag atgagcagca
     2161 cgagcacgaa cacgtggttc agcaaaatcg cgatcgccca gcacgtcgtc cgcgagctct
     2221 tccccgaagt gatgaacgac gccgcgcgcc gcggcgaccg cgttcacgcc gactggcagc
     2281 gcacgcccgg ttgcggcgaa cacgcgatgt gcgatcagat tcgcagcgac tccggcattg
     2341 tctgcggcag ccgctattat ccacgcggcg tctccgctta tctctggctc tctgagtagt
     2401 ctttccctca actccggccg ttcagcgagt gacccgtggt gaatccgcgg cactctcctc
     2461 ttcccacccc aaatcccatg agcgattcag cgcataagat ccccgttcgc gaaaaaatcg
     2521 gctacgccct gggcgacgta gcgacgaatt ttttcttcca gtcgatgatt ctctaccagg
     2581 cccgttttta tacggacacg gtaggactcg cgcctgcgat cgtcggttgg atgttcctca
     2641 tcgttcgcct cggcgacgcg ttcgtcgatc ccatcgtcgg tgcgctctcc gatcgcacgc
     2701 agtctcgctg gggtaaattc cgcccgtgga tcctatggac cgcgctcccg ttcggcctga
     2761 ttttctactt cgtttatgtc gcgccgaatt tcggcatgca gggcaagatc atctacgcgg
     2821 tcgttaccta cagtttgatg atggtgatct actcggcgaa caacacgccg tatgccgcgt
     2881 tgatgggcgt catgacgccc aatgtcagcg agcgcgccaa tatcgcgcgc taccgtttcg
     2941 tcgctgcgct catcggccag ttcatcatcc aggcgctcgc acttcccctc gtcgacaagt
     3001 tcggcgccgg caacagtgcc aaaggttggg cgaccacgat ggcgatcttc ggtgtgatca
     3061 tcgtggtgtg taacatcatc gtgttcttca ccacgaagga gcgcgtgctt cccaatcccg
     3121 agcagaagcc gtcgatcaag gaagacatca aaaacacctg gggctgcaaa ccctggcagg
     3181 cgatgttcgt cctgacgctc gcgatcttca cgatgctcgt cattcgcggc acctcgctga
     3241 attacctctt cacttactac ctcgatccgg cggcgatgac tcgcttcgtc gacaccatcg
     3301 ggctcgccgc ggcggccacc ggcggcgaac cgactggctt cgcctacgtt ctggatctgt
     3361 tcggacttct ggttcgcccc gacaactcca acgctcccgc cgtcgccttc gctttcttcc
     3421 aggtcctcgg ctcgatcatc caaatcatcg ccattccgtt ctcgaagccc ctctccgacc
     3481 gtttcggaaa gaaggcggtt ttcatcgcag gcatgagcgt cacgattctc gccaccttgg
     3541 gcgtcttcct cattgggccg accgacatga atctcatgtt ctggctcagc atcatctggg
     3601 gactcggttg gggtccgacc gtgccgctgc tgtgggtcat gatcgccgac gtcgccgact
     3661 attccgaatg gcaaacgtcc cgtcgcgcga ccggcttcat gtatgccggc atcctctttg
     3721 cgctgaaagc gggtctcggc cttggcggcg cggtgatcgg ctggatcatg gggcttcacg
     3781 gctatgtggc caacgtcgtc caatcggaat cggcgatgct gggcattcgc ctgggttcgt
     3841 ccgtctacgc cgcggttccc atgctcatcg ggctc
//
LOCUS       DASPLT010001053         1325 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_113908, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001053 DASPLT010000000
VERSION     DASPLT010001053.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1325)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1325)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1325
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_113908"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(145..>1325)
                     /locus_tag="VEA63_10910"
     CDS             complement(145..>1325)
                     /locus_tag="VEA63_10910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD84558.1"
                     /translation="AAMTDVETGAEVELGEGRVRFKDPLVRPQRAEHESESMNVAFAT
                     RKAAEEWMARQKASGADVRIVGEARKVRYFPGTQQKSLKLGGDADGLRAIGYIGQTFF
                     AHVFQEEARRSEMQAFKDYTLKGAGADLVWWEFGCADTRPNAFAFGHRVIVGLDAESG
                     HAYCRISLFSALDFAMILARLPIEASRAVVTDIDPLAKSPPNDIVVTQEASPLGAVAK
                     PTDTKAGLSAAISTGAAQRSLEALMRRLIDYERQNAAVRIADKVNTAASLSAADQENL
                     FAGIADEEAQRVLSLMGYAADDLTQRASSPLESKLADFMRAAVERDPYSDNGLTANAS
                     EMLGHACQALAKQMYTDYSAGALDAPRIVELFGTGNGAHIVATALSEVFVTRLMGGST
                     "
BASE COUNT          209 a          428 c          407 g          281 t
ORIGIN      
        1 ttgggcgtcg cccgcttcgc gggccgcgct ctcgtgactt cgccatcgag cggcgctcgc
       61 gcgcgtctcg acccttcggg cttcgatcgc tgacgctggg cgccttcatc ggttggcccg
      121 cgccttccgg cacgagctgt cttgctaggt cgaaccgccc attagtcgcg tcacgaagac
      181 ctcgctcaag gcggtggcga cgatatgagc gccattaccc gttccgaaga gctcgacgat
      241 gcgcggcgca tcgagcgcgc cggccgaata gtcagtgtac atttgttttg cgagcgcctg
      301 gcatgcgtgg ccaagcattt cgctcgcgtt agcagtgagg ccattgtcgg aatatgggtc
      361 gcgttcgacc gctgcgcgca tgaagtctgc gagtttgctt tcgaggggcg acgatgcgcg
      421 ttgggtgagg tcgtccgccg cgtagcccat gagactcaga actctttgcg cttcttcgtc
      481 tgcaatgcct gcgaagaggt tctcctgatc ggcggcgctg aggcttgcgg ccgtgttgac
      541 cttatcggcg attctcaccg ctgcgttctg ccgttcgtaa tcgatgaggc gccgcatgag
      601 cgcctccagg ctcctttgcg cagcgccggt ggagatcgcc gccgaaagcc cggccttcgt
      661 atcggtcggt ttggcaactg cgcccaaagg tgacgcttct tgcgtcacga caatgtcatt
      721 cggcggcgat ttggccagcg ggtcgatgtc cgtcacgacc gctctgctcg cctcgatggg
      781 cagacgagct agaatcatcg cgaagtcgag cgccgagaag agcgagatgc ggcaataagc
      841 atgaccgctt tcggcgtcga ggcctacgat caccctgtga ccgaaagcaa acgcgttcgg
      901 gcgcgtatcc gcgcaaccga actcccacca gacgagatcg gcgccggcgc ccttcaaggt
      961 gtagtccttg aaggcttgca tttccgagcg ccgcgcttct tcctgaaaca cgtgcgcgaa
     1021 gaaggtctga ccgatatagc cgatcgccct caagccatcg gcgtcgccac cgagcttaag
     1081 cgacttctgt tgggtgccgg gaaagtatct aaccttgcgt gcctcgccca cgattctgac
     1141 gtcggcccca gacgccttct ggcgcgccat ccattcctcc gccgccttgc gcgttgcgaa
     1201 ggcgacgttc atgctctcgc tctcgtgttc tgcgcgctgc gggcgcacga ggggatcctt
     1261 gaagcgtacg cgcccttcgc ccagttcgac ctctgcgccg gtttccacat cggtcattgc
     1321 cgcac
//
LOCUS       DASPLT010001054         1037 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_36238, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001054 DASPLT010000000
VERSION     DASPLT010001054.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1037)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1037)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1037
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_36238"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..584)
                     /locus_tag="VEA63_10915"
     CDS             complement(<1..584)
                     /locus_tag="VEA63_10915"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HYD84559.1"
                     /translation="MSRPAPGGLNTGVQGAFGSRHQPTATRAATSRLLFDARTYDTTR
                     HAMKQRTLGREVSIKGNALHTGDAVTLTLKPAPVDHGVVFKRVDLHGAPELKPRVDQI
                     TDLVRATTIQVGHAKIHTVEHVLSALHGCGVDNVLVEMDASEPPIMDGSARPFVNLIL
                     QAEPVEQEKDREYFELDSAVSVTRGNSSIIALPS"
     gene            640..>1037
                     /locus_tag="VEA63_10920"
     CDS             640..>1037
                     /locus_tag="VEA63_10920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007360291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HYD84560.1"
                     /translation="MPAANDIRRGQVIKFNGEPHLVMETQHRTPGNLRAFVQIKMRNL
                     RYGKALDQRFASTDTIEVLPTEKKTLEFSYADRDTYAFMDPDTFEQIELSAQTLGDTV
                     HYLTAGGKVDVLFVDERPLSVDLPSTVALKV"
BASE COUNT          200 a          367 c          282 g          188 t
ORIGIN      
        1 tcgctcggca gcgcgatgat cgacgagttg ccccgcgtca ccgacaccgc gctatccagc
       61 tcgaaatact cgcggtcctt ctcctgctcc accggctcgg cctgcaggat caggttaaca
      121 aacggccgcg ccgagccgtc catgatcggc ggctccgatg catccatctc caccagcacg
      181 ttgtcgacgc cgcagccgtg caacgcgctc agcacatgct caaccgtgtg aattttcgca
      241 tgcccgacct gaatcgtcgt ggcgcgcacg aggtcggtga tttgatcgac gcgcggcttc
      301 aattccggcg caccgtgcag gtcgacgcgt ttgaacacca cgccgtgatc caccggcgcg
      361 ggcttgaggg tcagcgtcac cgcatcgccg gtgtgcagcg cgttgccctt gatcgaaact
      421 tcccgcccga gtgtgcgttg cttcatagcg tgacgcgtag tgtcgtaagt gcgcgcgtcg
      481 aagagcaagc gcgatgtcgc tgcgcgagtt gcagtcggtt ggtgacgaga gccgaacgca
      541 ccctgcacgc cggtgttgag accgcccggc gccggccttg acaccgccaa aggcgtttac
      601 tcactctcga ccctcacatc catcgtaacc ttcatcgtca tgcccgcagc caacgacatt
      661 cgcagaggcc aagtcatcaa gttcaacggc gaaccccacc tcgtgatgga gacgcagcat
      721 cgcacgccgg gcaatctccg cgctttcgtg cagatcaaga tgcgcaatct ccgctacggc
      781 aaagccctcg accagcgctt cgcgtccacc gacaccatcg aagttctccc caccgagaag
      841 aagaccctcg agttcagcta cgccgaccgc gacacctacg cgttcatgga ccccgacacg
      901 ttcgagcaaa tcgaactcag cgcgcagacg ctcggtgaca ccgttcacta cctcaccgcc
      961 ggcggcaaag ttgacgtgct cttcgtcgac gaacgtccac tctccgtcga tctcccgtcg
     1021 accgtcgcgc tgaaagt
//
LOCUS       DASPLT010001055         1990 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_72744, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001055 DASPLT010000000
VERSION     DASPLT010001055.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1990)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1990)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1990
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_72744"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            39..>1990
                     /locus_tag="VEA63_10925"
     CDS             39..>1990
                     /locus_tag="VEA63_10925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD84561.1"
                     /translation="MSSKRGIETAGDEIRFTTESGRVIFGRGGELRAIENSEGRWQFA
                     ESGAFPRIELHYPKTDEQPETNVTFDDTVGQRTVQWSSGELFHKLRIRLAQAEGAALE
                     VEFRIPRHGREVTMTTAVFPGPRKNVAVRQNRLLEGRLTGGEGTARIVEVPAGIRRLL
                     RAEHAYTVSAVTTSASKSALLAVPLVIGGGNGEWSVDENGGVVLRGAGGLQRGDEGEK
                     NTLHAFWTEVRLVPTAAVEVEPLWQRYREHVQPLVAIVEEPGMAITHLNAALRAITGE
                     MKPIGWRQEAAHALVRDDVARMGRILKSGPKKHEADVAALLGGARAAQAKLTNNGERK
                     VLEHEKGRAYGGLDPYHVTYTQSAAAALSLFGEVPERVGAVNHAMARAVREFGGRTDA
                     FGSPYIDCFNRALNMQMGPMLFGLTAGAAAGDADIARFYRDLATAPAVQAVFGRGQRP
                     YSGAPAKTADQTDYLYQAICDFWLRTTELLANEDLQLHALAYSRYTDCVDVMADRYHG
                     VASRDKPGAAGQARANFFRGQAHTHRWLGWSAAPYIRLLETPEEGAAIGLTEAVRHTL
                     LQKGRWKNWPDLTFYALADLLVRKGLGRYERPNLPVHPTNVVTRADGRAIEISWDAVP
                     EAAEYRVYRADGEGGPYRWLNSSYVE"
BASE COUNT          389 a          541 c          716 g          344 t
ORIGIN      
        1 tgcgccgcaa agaaaattcg ccggcggccg tcgcgtcttt gtcatcgaag cgaggaatcg
       61 aaactgcggg tgacgagatc cgcttcacga cggagagcgg acgagtgatt ttcgggcgcg
      121 gcggcgagtt gcgtgcgatc gaaaattcgg aaggacgatg gcagttcgcg gaaagcggcg
      181 cgtttccgcg catcgagttg cattatccga aaacggatga acaaccggaa accaacgtca
      241 cgttcgatga tacggtcgga cagcgcacgg tccagtggtc gtcgggcgaa ttgtttcaca
      301 agctgcgcat tcggctggcg caggccgaag gcgcagcgct cgaagtggaa tttcggatac
      361 cacggcacgg tcgtgaagtg acgatgacga ccgccgtttt tcccggcccg cgaaaaaatg
      421 tggccgtgag gcaaaaccga ctgctcgagg ggcgattgac gggtggggag ggaacggcgc
      481 ggatcgtgga ggtgcctgcg gggatccgac ggttgttgcg agcggagcat gcttacaccg
      541 tgagcgcagt gacgacgtca gcgtcgaagt cggcgctgct ggcggtgccc ttggtgattg
      601 gcggcggaaa cggcgaatgg agcgtggacg agaatggcgg agtggtgctg cgcggggcgg
      661 gcggattgca gcggggagac gaaggcgaaa aaaacacgct tcacgcgttt tggacggagg
      721 tgcgactcgt gccgacggcg gcagtcgaag tcgaaccgct gtggcagcgt tatcgcgaac
      781 acgtgcagcc gctggtcgcg attgtcgagg agccgggcat ggccatcacg catctcaacg
      841 cggcgttgcg cgcgatcacc ggcgagatga agccgattgg gtggcgacag gaagcggcgc
      901 acgcgctcgt gcgcgatgac gtggcgcgca tgggacgcat tttaaaaagc gggccaaaga
      961 aacatgaagc cgacgtggcg gcattgctgg gcggcgcgcg cgcggctcag gcgaagttga
     1021 cgaacaacgg cgagcggaag gtgctcgaac acgagaaagg tcgcgcctac ggcgggctcg
     1081 acccgtatca tgtgacgtat acgcaaagcg cggcggcggc gctgtcgttg ttcggcgagg
     1141 tgcctgaacg ggtcggcgca gtgaatcatg cgatggcgcg ggcggtgcgc gaatttggcg
     1201 gacgcaccga tgcgttcggc agtccttaca tcgattgttt caatcgcgcg ttgaacatgc
     1261 agatggggcc gatgctcttt gggttaacgg ccggggccgc ggcgggcgac gcagatattg
     1321 cgcgttttta tcgcgatctc gcaaccgcgc cggcggtgca agcggtgttc ggtcgcgggc
     1381 agcgtcccta cagcggtgcg ccggcgaaaa ccgccgacca aaccgactat ctgtatcagg
     1441 cgatctgcga tttctggctg cgcacgaccg aactgctggc gaacgaagat ctacaactgc
     1501 acgcgctcgc gtattcgcgt tacacggact gcgtcgatgt gatggccgac cgctatcatg
     1561 gcgtcgcatc gcgggacaaa cccggtgccg cgggacaagc gcgcgccaac tttttccgcg
     1621 gccaggcgca cacgcatcgg tggctcggct ggtcggccgc gccgtatatc cgtttgttgg
     1681 agacgccgga ggaaggcgcc gcgatcgggc tgaccgaagc ggtgcgccac acattgctcc
     1741 agaaaggccg ctggaaaaac tggcctgatc tcacgttcta cgcactcgcg gatttattgg
     1801 tgcgcaaagg tctcgggcgt tacgagcggc cgaatcttcc cgtgcacccc acgaatgtcg
     1861 tgacgcgtgc ggacggccgc gcgattgaaa tctcctggga cgccgtgcca gaagcggcgg
     1921 agtatcgggt gtatcgcgcc gatggcgaag gcggacctta tcgctggttg aactcgtcgt
     1981 atgtcgagga
//
LOCUS       DASPLT010001056         1944 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_155660, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001056 DASPLT010000000
VERSION     DASPLT010001056.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1944)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1944)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1944
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_155660"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1922)
                     /locus_tag="VEA63_10930"
     CDS             complement(<1..1922)
                     /locus_tag="VEA63_10930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD84562.1"
                     /translation="MLPEMYPRFHVRAGEPVAARVYSLLALRVVFAGETGNPVGTMFG
                     AAGLGLDETKLLAMQVDDFFWENSAGLVQFAPTFTDVLTLPKTKAAYGADYLELVGDA
                     KLAAKAAGFDAETFDFTVILAPSADFPYAGISGGSWIHINGAFVANILVHELGHSFGM
                     GHSATWASNTSDPLAADGRRIEYGNPFDPMGQGHDAAQHFSAIWKERAGWWPEESVRR
                     VTTSGTTRVQRYDHFQASGVRVLVIPAGEGLEYWVSVARNHSASDFEIRTAPRDGVLI
                     YRLNTAQVGLEMPELIRPGDDGDDADDFFGARFVPGMSLIDAANKVKITVVAIGGASP
                     NEWADVKVEFAHTPALAFTLEPQDTTLAVGRTLTLRAESEVAATWQWQFNGVALSGET
                     SSTLTIASAQAANGGSYTVEATAGGKTVRSREAKVTIVDAPTITFETADQAVAEVESG
                     YRAYLGFRTAARNPSKGWWFRDGVEAYRFTYQANGAYSGNDSGAGWNSGDGDATFFDS
                     LVSFGWMTPGQAGFYSYVETNAAGAAESVPLSVGVRSHKKVLGDAMEFRSDVTHPNGN
                     VYDQLLMTGRAATLRADAGQVTRCSFIDLNDDIVQVEFSGRGNLTLVLDGVSGPARPV
                     NYNQAVEYVKGHATIV"
BASE COUNT          376 a          765 c          467 g          336 t
ORIGIN      
        1 atcacgatcg tcgcgtgccc tttcacatac tcgaccgcct ggttgtagtt cactggtcgc
       61 gccggcccgc tcactccgtc gagcacgagc gtgagattgc ctcgtcctga aaattccacc
      121 tgcacgatgt cgtcgttcaa atcgatgaac gagcaccgcg tcacctgccc cgcgtccgcg
      181 cgcaacgtcg ccgcccgccc cgtcatcaac aactggtcat acacgtttcc attcggatgc
      241 gtgacgtcgc tccgaaactc catcgcgtcg cccaacactt tcttgtgcga acgcacgccg
      301 accgacaacg gcaccgactc cgccgcgccc gcggcattcg tttccacata cgaataaaat
      361 cccgcctgcc ccggcgtcat ccaaccgaac gacaccagcg aatcgaaaaa cgtcgcgtca
      421 ccgtctcccg aattccaccc ggcgccactg tcgtttcccg aatacgcccc gttcgcctga
      481 tacgtgaaac gatacgcctc cacgccgtcg cgaaaccacc accccttcga gggattgcgt
      541 gccgccgtgc gaaaaccgag atatgcgcga tagccgctct ccacttccgc caccgcctga
      601 tccgccgtct caaacgtgat cgtcggcgca tccacgatcg tcaccttcgc ctcacgactc
      661 cgcaccgtct tcccgcccgc cgtcgcttcc accgtatagc tgccgccgtt cgccgcctgc
      721 gcgctcgcga tcgtcaacgt cgacgatgtt tctccgctca acgccacgcc gttgaactgc
      781 cactgccagg tcgccgccac ctccgactcc gcgcgcaagg tcaacgttcg ccccaccgcg
      841 agcgtcgtgt cctgcggctc gagcgtgaac gcgagcgccg gcgtgtgcgc aaactcaacc
      901 ttcacgtccg cccattcgtt cggcgacgcc ccgccgatcg ccacaaccgt gatcttcacc
      961 ttgttcgccg catcgatcag cgacatgccc ggcacaaaac gcgcgccaaa aaaatcgtcc
     1021 gcgtcatccc catcgtcacc cggacgaatc agctccggca tctccagtcc gacctgcgcc
     1081 gtgttgagcc gataaatcag cacgccgtcg cgcggcgccg tgcggatctc gaagtcgctc
     1141 gcgctgtgat tccgcgcaac gctcacccaa tactccaaac cttcgcccgc cggaatcacg
     1201 aggacccgca cgccgctcgc ctgaaaatga tcgtagcgtt gcacgcgcgt tgtccccgag
     1261 gtcgttacgc gccgcaccga ctcctccggc caccagcccg cgcgctcttt ccagatcgcc
     1321 gaaaaatgct gcgccgcgtc gtgcccctgt cccatcggat cgaaaggatt gccatactcg
     1381 atccgtcgcc cgtccgccgc gagaggatcg gacgtgttcg atgcccacgt cgcactatgg
     1441 cccattccaa acgaatgccc gagttcgtgc acgaggatgt tcgccacgaa cgcgccgttg
     1501 atgtgaatcc atgaacctcc cgaaattccc gcatacggaa aatccgccga cggcgcgaga
     1561 atcacagtga aatcgaacgt ctccgcgtcg aaccccgccg ccttcgccgc cagctttgcg
     1621 tcgcccacca attccaaata gtccgccccg taagccgcct tcgttttcgg cagcgtcaag
     1681 acatccgtaa acgtcggcgc aaactgcacc aacccggccg agttctccca aaaaaaatca
     1741 tccacctgca tcgcgagcag cttcgtctcg tccaacccca gccccgccgc tccgaacatc
     1801 gtgcccaccg gattccccgt ctcgcccgca aacacgaccc gcagcgccag cagcgaataa
     1861 acgcgcgccg ccaccggttc gcccgccctg acgtggaaac gtggatacat ctcgggcagc
     1921 acgagacacc gcccgtcacc cgcg
//
LOCUS       DASPLT010001057         1151 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_278352, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001057 DASPLT010000000
VERSION     DASPLT010001057.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1151)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1151)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1151
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_278352"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            7..144
                     /locus_tag="VEA63_10935"
     CDS             7..144
                     /locus_tag="VEA63_10935"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HYD84563.1"
                     /translation="MSKEIADRLGLSPRTVEKARAQILAKLGLRDLPSLVRWSLQHGL
                     S"
     gene            complement(320..>1151)
                     /locus_tag="VEA63_10940"
     CDS             complement(320..>1151)
                     /locus_tag="VEA63_10940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD84564.1"
                     /translation="NEEYRFYQVLLAAWPADDAEPDDTFRGRMREHWRKAVNEAKRNT
                     SWVQPNEPWLEAGERFLEGILNAETGREFLASFRDKARRVAHLGMVNSLAQMVLKAAS
                     PGVPDFYQGCELWDLSLVDPDNRREVDFARRQELVCAAERVDWRSLLANWESGAIKLN
                     VIAALLRFRGEEAELFRSGTYRPLEVTGRFADNVVAFAREAEGVCAVVIAPRLTSALG
                     CPPLGLVWGDTAVSAPAVLKGWRNAVTGREVAGGGELKLADLLGELPFAVLRGRTAVV
                     "
BASE COUNT          220 a          437 c          298 g          196 t
ORIGIN      
        1 ggcctgatct cgaaggaaat cgccgatcgc ctcggcctca gtcctcgcac cgttgaaaag
       61 gcccgcgctc aaatcctcgc gaaactcgga ttgcgcgacc ttccctcgct ggtgcgctgg
      121 tcgttacagc acggattaag ctgaaactcg gaacgtcgtc gcggatacga agagcgaaaa
      181 actccgaaaa attgcggccg tcgtcgcgat taccaaccct cgacgtgcgg tcggagggac
      241 cgccgtcgat tcgcaagcag gctcctacct acggctgcga ttctgtttga aacgctctaa
      301 cgctgcgccc acggctccgt cacaccaccg ccgtccgccc gcgcaacacc gcgaacggca
      361 gctcgccgag caaatccgcg agcttcaact cgccgcctcc agctacctcg cgccccgtca
      421 ccgcattgcg ccagcctttc aacaccgcgg gcgcgctgac cgcggtgtcg ccccacacga
      481 ggccgagcgg cggacaaccc agcgcgctcg tcaaccgcgg cgcgatcacc accgcgcaaa
      541 ccccttccgc ttcgcgcgcg aacgccacca cgttatcggc aaagcggccc gtgacttcca
      601 gcggtcgata tgttccgctc cggaacaact ccgcctcctc gccacgaaaa cgcaggagcg
      661 ctgcgatcac attcaacttg atcgcaccgc tctcccaatt cgccaacagc gaacgccagt
      721 cgacgcgctc cgccgcgcac acgagttcct gcctccgcgc gaagtccact tcgcgccgat
      781 tgtcgggatc gaccaaactc aggtcccaca attcgcagcc ctgataaaaa tccggcacgc
      841 ccggcgatgc agccttgagc accatttgcg cgagcgagtt caccattccc aggtgcgcta
      901 cgcgccgagc cttgtcgcgg aaactcgcca gaaactcgcg tcctgtttcc gcgttcagaa
      961 tcccttcgag aaaccgttcg cccgcttcga gccacggctc gttgggctgc acccacgagg
     1021 tgttccgctt cgcttcgttc accgccttcc gccagtgctc gcgcatccgt ccgcgaaacg
     1081 tgtcgtcggg ctccgcatcg tccgccggcc atgccgcgag cagcacctga tagaaacgat
     1141 actcctcgtt c
//
LOCUS       DASPLT010001058         1287 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_278432, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001058 DASPLT010000000
VERSION     DASPLT010001058.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1287)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1287)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1287
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_278432"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..342)
                     /locus_tag="VEA63_10945"
     CDS             complement(<1..342)
                     /locus_tag="VEA63_10945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD84565.1"
                     /translation="MSSPHTRPWRDAALATASVLLVALIGLGLSYRAARVAQIEAVQN
                     ELQQLAATLAAQVDGDLHRTIRSEAQSGTPEHLRALEPIGRFLNSAKDIIYAYTAVLR
                     GDEIRFVLNGSF"
     gene            complement(749..>1287)
                     /locus_tag="VEA63_10950"
     CDS             complement(749..>1287)
                     /locus_tag="VEA63_10950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HYD84566.1"
                     /translation="NQAEVDYVRGHVGLVITGANETSNISIMSVGPITAVNQTLFKED
                     VIYDGVADIAFIAIQSANGKFGGIRTGNVSYYASQGFTGVYAPGVEVTGPVNIGDVNA
                     FDSATPVLVFGSVATVSVTGGSLEQDNDAPVTVSGIAQVRFVSGTTSQGVALAAQANA
                     GVLMEDGVDVTDLIVVNP"
BASE COUNT          243 a          424 c          389 g          231 t
ORIGIN      
        1 gaacgagccg ttgagcacaa aacgaatttc gtcgccccgc agcacggcgg tgtaggcgta
       61 aatgatatcc ttggccgagt tgagaaagcg gccgatgggc tcgagcgcgc gcaggtgctc
      121 gggcgtgccc gactgcgcct ccgagcgaat cgtgcgatgc aaatcgccgt cgacctgcgc
      181 cgccagcgtg gccgcgagtt gctgaagttc gttttgcacc gcttcgatct gcgccactcg
      241 cgcggcacgg tagctgagcc caagaccgat cagcgcgacc aagagcaccg aagcggtcgc
      301 aagcgcggca tcgcgccagg ggcgagtgtg aggcgaagac acgagcgtca ttctggaaag
      361 tggacggata aagtcggcgc ggacgaaagc ggccgacctt ttgatcgcac cgacggccgg
      421 caagccatcc atttcgaaaa gacgacgctt ccttacccga aaaaaaaagc ccgcagcgtc
      481 gcgccgcggc tgaaaccgga tgaatcacac ctcgttaaag gtgcgtccca gtccgcaaga
      541 tggagccgcg gcgccatgat ttatccggct agctgtagga gaggctttat gccccgaagt
      601 cgccgtagcg gcaggcctcc gtgcctgcct gcttttcggt tcggaattga tgttcgaaat
      661 gtaggtggcg cgcttgccgc gccacgttgc gcgcgagcgc gcaacctaca atccaggcag
      721 gcaggcacgg aggcttgccg ctacgcgatt acggattcac cacaatcagg tcggtgacgt
      781 cgacgccgtc ctccatgagc acgccggcgt ttgcctgcgc cgcgagcgcc acgccctgcg
      841 aggtggtgcc gctcacgaat cgcacttgcg cgatcccgct cacggtcacc ggcgcgtcgt
      901 tgtcctgctc caggctgccg cccgtcaccg acaccgtcgc cacgctgccg aacaccagca
      961 ccggcgtcgc cgagtcgaac gcgttgacgt ctccaatgtt caccgggccc gtcacctcca
     1021 cgcccggtgc atacacgccg gtaaaaccct ggctcgcgta gtagctcacg ttgcccgtgc
     1081 gaatgccgcc aaactttccg ttcgcgctct gaatcgcgat aaacgcgatg tccgccacgc
     1141 cgtcgtagat gacgtcttcc ttgaagaggg tttggttcac cgccgtgatc ggccccacgc
     1201 tcatgatcga gatattgctc gtttcatttg ctcccgtgat cacgaggcca acgtggccgc
     1261 gcacgtagtc cacctccgcc tggttgt
//
LOCUS       DASPLT010001059         1304 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_236774, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001059 DASPLT010000000
VERSION     DASPLT010001059.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1304)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1304)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1304
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_236774"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..342
                     /locus_tag="VEA63_10955"
     CDS             <1..342
                     /locus_tag="VEA63_10955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD84567.1"
                     /translation="DLDEMEALLDAREALLDSVRASETDRRGVVTVREAEALQQLKAE
                     LERQEASLREARAALREREKFLEESETRLFAKVQEQQEKETELEQREENLASRAASPR
                     PAPAPFDEFRE"
     gene            complement(556..>1304)
                     /locus_tag="VEA63_10960"
     CDS             complement(556..>1304)
                     /locus_tag="VEA63_10960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007359480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HYD84568.1"
                     /translation="IGIGDAPNGGNINAAVGSEHPEQLAATVRASGARLGVAHDGDGD
                     RCVLCDERGAVLDGDEILAILATHALQRGRLVKQTLVITVQSNLGVDAAIQAAGGRVC
                     RTAVGDRYVIERMLAEGATLGGESSGHIICAEISPTGDGLVAALKVFEVMLTTGRPLS
                     ELRRVLRKFPQQTAALKVKEKKPLETLPGVNEVIRALEAELGGQGRVLVRYSGTEAKL
                     RLLVEGPTDAVVQSGIARLEAAVRTELAVV"
BASE COUNT          226 a          513 c          341 g          224 t
ORIGIN      
        1 gatctcgacg aaatggaagc gctgctcgac gcccgcgaag cgctcctcga ctccgttcgc
       61 gcttccgaga ctgaccggcg cggcgtcgtg accgtgcgcg aagccgaggc gcttcaacag
      121 ctcaaagccg agctcgagcg ccaggaagcc agcctgcgcg aagcgcgtgc cgccctgcgc
      181 gaacgcgaaa aatttctcga ggaaagcgaa acccgcctct tcgccaaagt gcaggaacag
      241 caggaaaagg aaaccgagct cgagcagcgc gaagaaaacc tcgcctcccg cgccgcctcg
      301 ccccgccctg ctcccgcgcc tttcgacgaa ttccgcgaat aatcctgtag ggccgccgcg
      361 ccgttaacgc taacgtttca ccttcgcgcg gaacgtcccc tccccatcgc ctccatcacc
      421 gacgttttgc gccagcgcga gggagctggg tagggcggac tttagtccgc ctttcgcggc
      481 ggagcgggct gaagcccgcc ctactttgct ctactttttc caggtgcctt gggcgtcaac
      541 gccggattca ccagctcaca ccaccgccaa ctccgtccgc accgccgctt cgagtcgcgc
      601 gatcccgctt tgcacgaccg catcggtcgg cccttccacc agcagccgca gtttcgcctc
      661 ggttcccgaa tagcgcacca acacgcgtcc ttgtccgccc aattccgcct cgagcgcgcg
      721 aatcacttca ttcacgcccg gcagcgtttc gagtggcttc ttttccttca ccttcaacgc
      781 cgccgtttgt tgcggaaatt ttcgcagcac ccgccgcaat tccgacagcg gccgccccgt
      841 cgtcagcatc acctcgaaca ctttcaacgc cgcgaccaat ccgtcgccgg tcggagaaat
      901 ctccgcgcaa atgatatgcc cgctcgactc gccaccgagc gtcgcgcctt ccgccaacat
      961 ccgctcgatc acgtagcgat cgcccaccgc cgtccggcac acccgaccgc ccgccgcctg
     1021 aatcgccgcg tccacgccga gattactctg cacggtgatg accagcgtct gcttcaccaa
     1081 ccttccgcgt tgcaacgcat gcgtcgccag aatcgcgagg atctcatccc catcgagcac
     1141 cgcgccccgc tcgtcgcaca acacgcagcg gtcgccgtcg ccatcgtgcg ccaccccgag
     1201 ccgcgcgccg ctcgcgcgca ccgtcgccgc gagttgctcc ggatgctcgc tgccgaccgc
     1261 cgcattgatg tttcctccgt tcggcgcgtc gccgatcccg atca
//
LOCUS       DASPLT010001060         1146 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_72833, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001060 DASPLT010000000
VERSION     DASPLT010001060.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1146)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1146)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1146
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_72833"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            138..1043
                     /locus_tag="VEA63_10965"
     CDS             138..1043
                     /locus_tag="VEA63_10965"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HYD84569.1"
                     /translation="MKTILLVDDNPANLGVLIELLRNSGYQLRVAESGERALVQLRHT
                     PADIVLLDVMMPGIDGLETCRRLKADPAWSDLPVIFMTALDDVVDKVAGFAAGAVDYI
                     TKPLQPEEVLARVRTHLRLRELQTELEERNERLDAAIRLRVAAEQALSDSLDRAVIVV
                     NGNDDVVFCTKSASRLLTRYFPGAVLSRVPAGLRLAPSNGVPLRVTSLLRERTSNFQL
                     LALEPVRSDPVPRDLETLGLTPREAEVLFWIAQGKTNSEVAVILATSINTIKTHSQRI
                     FEKLGVETRTAAALTAAEHLGQPLA"
BASE COUNT          203 a          378 c          351 g          214 t
ORIGIN      
        1 ttttacagga gggcgcagag gaagcagaga gaaagagccg tgctaaaact cactgattgg
       61 gccagatctt tttaagatct gcgggcaatt cctctgctac ctctgcttcc tcctgtaaaa
      121 tccgtcgtcg ccacgccatg aaaacgatcc ttctcgtcga cgacaatccg gcgaacctcg
      181 gtgtgctgat cgagctgttg cggaattccg gttatcaact gcgcgtggcg gaaagcggcg
      241 agcgcgcgct cgtgcagctc cgccacacgc cggccgacat cgtgctgctc gacgtgatga
      301 tgccgggtat cgacggtttg gagacgtgcc gccgcttgaa ggccgatccg gcgtggtcgg
      361 accttccggt gatcttcatg accgcgctcg acgacgtcgt cgacaaagtc gcgggcttcg
      421 ccgccggcgc ggtcgactac atcacgaagc cgctccagcc ggaggaagtg ctcgcgcgcg
      481 tgcgcacgca cctgcgtttg cgcgagctgc aaaccgagct cgaggagcgc aacgaacgcc
      541 tcgacgccgc gatccgcctg cgcgtcgccg cggagcaggc gttgtccgat tcgctcgacc
      601 gcgcagtgat tgtcgtcaac ggcaatgacg acgtggtctt ctgcacgaag tcggcgtcgc
      661 ggttgctcac gcgctatttt cccggtgcgg tgctgtcgcg cgtgccggcg ggtttgcgcc
      721 tggctccctc caatggcgtg ccgcttcgcg tgacctcgct gctgcgcgag agaacgagca
      781 atttccaact gcttgcgctc gaacccgtgc gctccgatcc cgtgccgcgc gatctcgaga
      841 cgcttggcct cacgccgcgc gaagcggagg tattgttctg gatcgcgcag ggaaaaacca
      901 actccgaagt cgcggtgatt cttgcgacgt cgatcaacac cataaaaacg cactcgcagc
      961 gcatcttcga gaaactcggc gtggagaccc gcaccgccgc cgcgctcacc gccgcagagc
     1021 atctcggcca gcccctggct tgagtcattc gtagcggcag gcctccgcgc ctgcctgccc
     1081 gctgtaggtt gcgcgctcgc gcgcaacgtg gtgcgcaagc gcaccaccta cccgtctaga
     1141 aaacaa
//
LOCUS       DASPLT010001061         1037 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_136164, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001061 DASPLT010000000
VERSION     DASPLT010001061.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1037)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1037)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1037
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_136164"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..380)
                     /locus_tag="VEA63_10970"
     CDS             complement(<1..380)
                     /locus_tag="VEA63_10970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007361089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HYD84570.1"
                     /translation="MSLSYESSGVRYDQLDAFKRACQKAARTTAGVLEDHGYSEPATT
                     RGESAYLIEAKDHFLAHVEEGLGTKNLVADAVYAATGKCFYREIAIDTVATIVNDLIT
                     CGALPISVAMHAAVGDSDWFADATR"
     gene            complement(473..>1037)
                     /locus_tag="VEA63_10975"
     CDS             complement(473..>1037)
                     /locus_tag="VEA63_10975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HYD84571.1"
                     /translation="TYGSVRPGDNLVCVDDSIVRGTTLRKSILRMLARLNPKKIVIVS
                     TAPQIRYPDCYGIDMSEIGKFIAFEAAIELLRERGQGGLLEEIYRACRDEIAAGGAVN
                     HVRKVYEPFTPEEISAKITERVRPQGIEWKGEIEIIFQTIESLHKAVPKHTGDWYFTG
                     KYPTPGGYRVVNQAFVNYYEKSEGRAY"
BASE COUNT          208 a          356 c          262 g          211 t
ORIGIN      
        1 cgcgtcgcat ccgcaaacca gtcggaatca ccgacggccg cgtgcatcgc caccgaaatc
       61 ggcagcgccc cgcaggtgat gaggtcgttt acgatcgtcg cgaccgtatc gatcgcgatc
      121 tcgcgataaa aacatttccc cgtcgccgcg tagaccgcgt ccgccacgag gttcttcgtg
      181 ccgagccctt cctcgacatg cgccagaaaa tgatccttcg cctcgatcag atacgcgctc
      241 tcgccccgcg tggtcgccgg ctcggaatag ccatggtctt cgagcacgcc cgccgtcgtc
      301 cgcgccgctt tctgacacgc gcgtttaaac gcatcgagct gatcgtaacg aacgccggaa
      361 gactcgtagg aaagactcat ctcagaagaa caaccacgaa tggacacgaa ttaacacgaa
      421 taattcagaa ctccggcatt cgtgtgcatt cgtctccatt cgtggtttca aatcagtaag
      481 cccgcccttc gctcttctcg tagtagttca cgaacgcctg gttcaccacg cgataaccgc
      541 ccggcgtcgg atatttgccc gtgaaatacc agtcgcccgt atgcttcggc accgccttgt
      601 gcaggctctc gatcgtctgg aaaataatct cgatctcgcc cttccactcg atgccctgcg
      661 gccgcacgcg ctccgtgatc ttcgccgaaa tttcctccgg cgtgaacggc tcgtagacct
      721 tccgcacgtg attcaccgcg ccacccgccg cgatttcatc gcgacacgcg cggtaaattt
      781 cctcgagcaa gccgccctgg cctcgctccc gcaaaagctc gatcgccgcc tcgaacgcga
      841 tgaattttcc gatctccgac atgtcgatgc cgtagcaatc cggataacgg atctgcggcg
      901 ccgtcgatac gatcacgatc ttcttcgggt tcagccgcgc cagcattcgc agaatcgatt
      961 tgcgcagcgt cgtgccgcgc acgatcgagt cgtcgacgca cacgagattg tcgccgggcc
     1021 gcaccgaacc atacgtg
//
LOCUS       DASPLT010001062         2418 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_236790, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001062 DASPLT010000000
VERSION     DASPLT010001062.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 2418)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2418)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2418
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_236790"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            26..>2418
                     /locus_tag="VEA63_10980"
     CDS             26..>2418
                     /locus_tag="VEA63_10980"
                     /inference="COORDINATES: protein motif:HMM:NF037085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBG domain-containing protein"
                     /protein_id="HYD84572.1"
                     /translation="MPSLSRFYGDENSFTVNFNGLVNSDTSSVVSGLTVSSSAATNSN
                     VGSYAIGASGAIADNYSINYSLGSLTVVPAPLTVSIDDATRRYGDADPVFTYSIGGLK
                     NNDTAGIVTISNLSSLATADAGIGDYGIIGFPFVSSPNYTAPAQVSGTLTITPRPLTI
                     TADDATRVYGDRNPALTATFTGLASFDTPALFGDLGLTTQATQSSGIGNWGITFPVVT
                     NRNYAITSQFGTLTITPAPLSIVIADAQRAYGQANPLFTSTLLGLKNDDTAGVVSGLT
                     LSTVARQLSDAGTYAITGSGTSLNYALTFVPGTLTVDRLSATIRLNNAGRIFGDSLTS
                     FLASYAGFLPADLSLAQSAWLADAPDDPLLRPGSYAVAIEEASPEARAAILKNYDVNF
                     VPGQLTIARKVVEVKAVDVRSDTTAVSNAFPVEIFGLVPFGPSFTAVAGSNVGNSAPP
                     GNYPIVPVITEVVGMSVEELNHYYDFRMTPGVLTLVVPLVVQDVVDLNNPGVLLQNTT
                     NHPLIVTDVFFENETIEVKQDTITVQQNVGFVDYSTNPLTQAEYADYFGSYIDDAETV
                     RTALASGYKSLLSGKGVKGSSYENMPADVRELLAGWMDGSLEPSAFQALIAQGDANAI
                     AAFSFILPSLVEMTLSKDMDEMTSLDREVLGRLADLTEKRHSDTVRIAQQKYEAMLET
                     QRKKAEIGGMGTLFTGPGDFKNIVQSATEEALSTYILASAGAVGGVAGTVGILTIPAI
                     GNAIFVNSGVAVMAGASGGFAIAATMMVCLAVRAVQIDESIKNEKAYHDLMSTWRLG"
BASE COUNT          473 a          754 c          717 g          474 t
ORIGIN      
        1 cgagcgcgcg ccgctcacga tcgcggtgcc aagtctatcg cgcttctacg gcgacgagaa
       61 cagcttcacg gtcaatttca acggcctggt caacagcgac acttcgtcgg tcgtgagcgg
      121 cctcacggtt tcatcttcgg cggcgacgaa ttcgaacgtc ggcagctacg ccatcggcgc
      181 gtcgggtgcc atcgcggaca actatagcat caactactcg ctcggctcgc tcaccgtcgt
      241 gccagcgccg ctcaccgtga gcatcgatga cgccactcgc cgctacggcg acgccgatcc
      301 tgttttcacg tattcgatcg gcggtttgaa aaacaacgac accgccggga tcgtgaccat
      361 cagtaatctt tcgtcactcg cgacggccga tgccggcatc ggcgattacg gcatcatcgg
      421 cttccctttt gtttcttcgc ccaactatac ggcgccggcg caagtctccg gcacgctgac
      481 catcacgccg cgtccgctga cgattaccgc tgacgacgcg acgcgcgttt acggcgaccg
      541 gaacccggcg ctcaccgcca ccttcaccgg tcttgcttcc ttcgatacgc cggcgctctt
      601 cggcgatctt gggctgacca cgcaggcgac gcagtcatcc ggcatcggca actggggcat
      661 caccttcccg gtggtgacca accggaacta tgcgatcacg tcgcagttcg gcacgctgac
      721 gatcacgcct gcgccgctga gcatcgtcat cgcggatgcg cagcgcgctt acggtcaggc
      781 caacccgctc ttcacttcga cgctgctcgg cctgaaaaac gatgacaccg cgggagttgt
      841 ttccgggctg acgctatcga ccgttgcgcg acaattgtct gacgcgggca cctacgcgat
      901 caccggcagc ggaacttcgc tcaactatgc gctgacgttt gttccgggca cgctgacggt
      961 cgaccgcctt tcggcgacga tcaggctcaa caacgccggc cgcatcttcg gggacagcct
     1021 tacttcgttt ttagcgagct acgccggttt ccttccggcc gatctttctc tcgcgcagtc
     1081 cgcctggtta gcggatgccc cggatgatcc cttgctgcgt ccgggttcgt atgccgttgc
     1141 gatcgaggaa gcgtcaccgg aagcgagagc tgcgattctc aaaaactacg acgtcaactt
     1201 cgtgcccgga cagctcacga ttgcgcgaaa agtggtcgag gtgaaggcgg tggatgtgag
     1261 atccgacacg acggcggtct ccaacgcttt cccggtggaa attttcggac tcgtcccctt
     1321 cggcccgagt ttcaccgccg tggcggggag caatgtcggc aactccgccc cgccgggaaa
     1381 ctacccgatc gtgccggtga tcacggaagt cgtcggcatg tcggtggagg aactgaacca
     1441 ctactacgac tttcggatga ctcccggcgt gctgacgctc gtcgtgccgc tggtggtgca
     1501 ggacgtcgtg gatctcaaca acccgggcgt gttgttgcag aacacgacga atcacccgct
     1561 gatcgtgacg gacgtctttt tcgagaacga gacgatcgag gtgaagcagg acacgatcac
     1621 ggtccaacag aacgtgggtt tcgttgatta cagcaccaat cctctcaccc aggcggaata
     1681 cgccgactat tttggcagtt atatcgatga tgcggagacg gtgcgaacgg ctctcgcctc
     1741 cggctataaa tccttgctca gcggcaaggg agtaaaaggc agcagctacg agaacatgcc
     1801 ggcggacgtg cgcgagctgc tcgccggatg gatggacgga agcctcgagc cgtccgcatt
     1861 tcaggcgctg attgcgcaag gagacgcgaa cgcgatcgcg gcctttagct tcattctacc
     1921 cagcttggtg gaaatgacgc tgagcaagga catggacgag atgacttcgc tggatcgaga
     1981 agtgctcggt cgcctcgccg acctcaccga aaaacgtcat tccgacaccg tgcggatcgc
     2041 gcagcagaaa tacgaagcca tgctcgagac tcagcggaag aaggccgaga tcggtgggat
     2101 gggcacgttg ttcacgggcc cgggcgactt caaaaacatt gtgcagagcg ccacggagga
     2161 agccctctcc acttacatcc tggcctcagc gggtgccgtc ggcggtgtcg ctgggaccgt
     2221 tggcattctg accatacccg ccatcggaaa cgccattttc gtgaattcgg gcgtggcagt
     2281 gatggctggc gccagcggcg gtttcgccat cgctgcgacg atgatggtct gcctggccgt
     2341 gcgtgccgtg cagatcgacg aaagcataaa aaacgaaaag gcctaccatg atttgatgag
     2401 cacctggcga ctcggcca
//
LOCUS       DASPLT010001063         1066 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_177283, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001063 DASPLT010000000
VERSION     DASPLT010001063.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1066)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1066)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1066
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_177283"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1066
                     /locus_tag="VEA63_10985"
     CDS             <1..>1066
                     /locus_tag="VEA63_10985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine dehydrogenase
                     (aminomethyl-transferring)"
                     /protein_id="HYD84573.1"
                     /translation="ALARRSAFLGHATFNRYHTEHEMLRYIKRLEAKDLSLVHSMISL
                     GSCTMKLNATSEMFPVSWPEFGKLHPFAPAEQTRGYQKLFRDLETWLSEITGFAAVSL
                     QPNAGSQGEYAGLLTIRAYHESRGQGQRSICLIPTSAHGTNPATAAMCGFKVVPVACD
                     ANGNIDVTDLRAKASVHANDLAALMVTYPSTHGVFEGSIKDICAIVHEHGGQVYMDGA
                     NMNAQVGLTSPGHIGADVCHLNLHKTFCIPHGGGGPGMGPIGVAKHLAPFLPGHSLVR
                     PHLSTLNAQLRAEGAVSAAPFGSASILVISWMYIRMMGPTGLTEATKFAILKANYVAK
                     RLEKFFPVLYRGNAGLIAHEC"
BASE COUNT          200 a          391 c          285 g          190 t
ORIGIN      
        1 gccctcgcgc ggcgcagcgc tttcctcggg cacgcgacct tcaatcgtta tcacaccgag
       61 cacgagatgc tccgctacat caagcggctc gaagcgaagg atctttcgtt ggtgcactcg
      121 atgatctcgc tcggctcgtg cacgatgaag ctcaatgcca cgagcgaaat gtttccggtc
      181 tcgtggcccg agttcggcaa attgcacccc ttcgcgccgg ccgaacagac gcgcggctac
      241 cagaaacttt tccgcgacct cgaaacctgg ctcagcgaaa tcaccggctt cgccgcggtc
      301 tcgctgcaac ccaacgccgg ctcccaaggt gaatacgccg gcctgctcac gatccgcgcg
      361 tatcacgaat cccgcggcca gggccagcgc agcatctgtc tcattccgac gagcgcacac
      421 ggcaccaatc ccgccaccgc cgcgatgtgt ggtttcaaag ttgtgccggt tgcctgcgat
      481 gcgaacggca acatcgacgt caccgatctg cgcgcgaaag cctccgtgca cgcgaacgac
      541 ctcgcggcat tgatggtcac ctatccgtcg acgcacggcg tgttcgaagg cagcatcaag
      601 gacatctgcg ccatcgtgca cgagcatggc ggacaggttt acatggacgg cgccaacatg
      661 aacgcgcaag tcggcctcac ctcgcccggc cacatcggcg ccgacgtctg tcatctgaat
      721 ctccacaaga cgttctgcat tccccacggc ggcggcggtc ccggcatggg cccgatcggc
      781 gtcgcgaaac acctcgcgcc cttcctcccc ggccactcgt tggttcgccc tcacctctca
      841 acgctgaacg ctcaactccg cgccgaaggt gccgtcagcg ccgcaccctt cggctccgcc
      901 tccatcctcg tgatctcgtg gatgtatatc cgcatgatgg gcccgactgg cctcaccgaa
      961 gccacgaagt tcgcgattct caaagccaac tacgtcgcca agcgcctcga gaagttcttc
     1021 cccgtcctct accgcggcaa cgccggcctg atcgcccacg agtgca
//
LOCUS       DASPLT010001064         1342 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_36463, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001064 DASPLT010000000
VERSION     DASPLT010001064.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1342)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1342)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1342
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_36463"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(29..1237)
                     /locus_tag="VEA63_10990"
     CDS             complement(29..1237)
                     /locus_tag="VEA63_10990"
                     /inference="COORDINATES: protein motif:HMM:NF015418.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HYD84574.1"
                     /translation="MLTPAEAEQRILSNLPLFLREDCPLLAAHGRVLREDVCADRDLP
                     PFDRVTMDGYALRSAAVAAGVTTFRVEGTQAAGMRARKLGDAIDVCVEVMTGAVLPDG
                     ADCVVPYEEAKKEDATISLSPDAVAKPGVSVHRRGSDRREGEVVLAAGTRLTGREIAI
                     AAAVGATNVTVTALPKIAILTTGDELVEVDSPVAAHQIRRSNDYALRAALIASGYPRV
                     ERFHLCDVRHEIEHLLWHVIAEHDGVLITGGVSKGKFDFLPAALEAQRVRKVFHGVAQ
                     RPGKPFWFGLSARRTPVFALPGNPVSAYTCLHRYVLPALARASGASAADHKLSAALTA
                     PVTFKPPLAYLLPVKISSGPDARLLAAPEPTNTSGDFGGLLNSDGFVELSADRTEFPA
                     GTVANFWRWV"
BASE COUNT          262 a          431 c          415 g          234 t
ORIGIN      
        1 cctcgttcgg gcgccacgta aagccgctct acacccaacg ccagaaattc gcgacggtgc
       61 cggcgggaaa ctccgttcga tccgcggaaa gttcgacgaa gccgtcactg ttgagcaatc
      121 ccccgaaatc gccagaagtg ttggtcggct ccggcgcagc gagcagtcgc gcatcggggc
      181 cgctggagat tttcaccggc aacagatacg ccagcggcgg cttgaacgtc accggtgctg
      241 tcagcgcggc gcttaactta tgatccgcgg cacttgcgcc gctcgctcgc gcgagcgcgg
      301 gcagcacgta gcgatgcaag cacgtatacg cggacacggg attgcccggc agcgcaaaca
      361 ccggggtgcg tctcgcgctc agtccgaacc agaacggttt acccggtcgc tgcgcgacgc
      421 cgtggaatac tttgcgcacc cgctgcgctt cgagcgcagc ggggagaaaa tcgaattttc
      481 ctttggaaac gcctcccgtg atcaagaccc cgtcgtgctc ggcgatcacg tgccaaagca
      541 agtgttcgat ttcgtgacgc acatcgcaca gatgaaaacg ttcaacgcgc ggataaccgg
      601 atgcgatcaa cgcagcgcgc agcgcgtaat cgttgctgcg ccgaatctga tgggcggcga
      661 caggagaatc gacttcgacg agttcgtccc cggtcgtgag aatcgcgatc ttcggcagcg
      721 ccgtgacggt aacattcgtt gcacccacag cggcagcgat cgcgatctcg cgtccggtca
      781 gccgtgtgcc ggcggcgagc accacttcgc cttcgcgacg atcgctgccg cggcgatgca
      841 ccgacacgcc tggtttcgcg accgcgtcgg gcgaaaggga aatcgttgcg tcctcttttt
      901 tcgcttcctc gtaaggcacc acgcagtccg cgccgtccgg caacacggcg ccggtcatga
      961 cttcaacaca cacatcgatc gcgtcgccga gtttgcgcgc gcgcatgccg gcggcctgcg
     1021 tgccttcgac gcggaaagtc gtcacgcccg cggcgactgc agcggatcgc agtgcatagc
     1081 cgtccatcgt cacacgatcg aacggcggca gatcgcggtc ggcgcacaca tcttcgcgca
     1141 acacccgtcc atgagcggcg agcagcggac aatcttcgcg caagaaaagc gggaggttgg
     1201 aaagaatgcg ttgttccgct tcagccgggg tgagcatggt gcgagttgta ggagacaggc
     1261 tttacgccgc gatcggtcgc ttcatcgtga cggtcgcgac gcgaagccgc tccttgatgc
     1321 agaatcccat cgaccagttc aa
//
LOCUS       DASPLT010001065         1170 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_278454, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001065 DASPLT010000000
VERSION     DASPLT010001065.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1170)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1170)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1170
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_278454"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..652)
                     /locus_tag="VEA63_10995"
     CDS             complement(<1..652)
                     /locus_tag="VEA63_10995"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HYD84575.1"
                     /translation="MLSDLRFTFRQIARFPGYTAVVVLTLAFGIAVNTQIFAIVSAMF
                     FQPMPVRDADRLVTIVLRNDLINLPMPVSILDFQDMRADSKALTEHIAFFSTPAHVGM
                     PGKSPERIGIEAVTPDAFAKYGVHAALGRTLQPSDGEMPPGTPVAMLSHAYWQTRYGG
                     APDILGRTILINAKPFTIVGVARPGFGSFSYMLASSVFIPSGAVAQIRPDGSDLFKY"
     gene            complement(947..>1170)
                     /locus_tag="VEA63_11000"
     CDS             complement(947..>1170)
                     /locus_tag="VEA63_11000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HYD84576.1"
                     /translation="RVLALTALGLVIGAAGAWGLGRFLASFMPRLAMADPVGLIAAAI
                     LLFVVAVIASFVPARRATKVDPLTALRAE"
BASE COUNT          239 a          317 c          431 g          183 t
ORIGIN      
        1 gatacttgaa gagatcactg ccgtcggggc ggatctgcgc gactgccccg gaagggatga
       61 agacggagct cgcaagcata taggagaaac tgccgaaacc cggtcgagcc accccgacga
      121 tggtgaacgg cttggcgttg atcaggatcg tgcggcccag gatgtcaggc gcgccgccgt
      181 atcgcgtctg ccaatacgcg tgcgagagca tggcgaccgg cgtgcccgga ggcatctcgc
      241 cgtcgctcgg ctgaagcgtg cggccgagcg cggcgtgcac gccgtatttg gcgaatgcgt
      301 ccggcgtcac cgcttcgatg ccgatccgtt cgggagattt tcccggcatg ccgacatgcg
      361 ctggggtcga aaaaaaggcg atgtgttccg tgagtgcctt ggagtcggcg cgcatgtctt
      421 ggaaatcgag gatcgagacc ggcatcggca ggttgatgag gtcgttgcgt agcacgatcg
      481 tgacgagtcg gtcggcgtcg cgcacgggca tcggctggaa aaacatcgcg ctaacgatcg
      541 cgaaaatctg cgtgttgacc gcgatgccga aggcgagcgt gaggacgacc acggcggtgt
      601 agccggggaa gcgcgcgatc tggcggaagg tgaaacgaag atcggagagc atggctttaa
      661 tgggacggcg gtctgggcgg cagggacgac gtcgcagtgt attgcacagt gcgtgccgga
      721 aaacacggga gcgatgtgag tcagcgtgtt ggagggactt acgtttccat ctatttcagc
      781 gaaacttcat cggcgctcgg aaacgaaacc gcggtttccc atgtgtggga cgccgcccca
      841 aacgaaacag acgccgggtt gcggcagaag aaaagggtag aggcggtcgt ctcgaccgcc
      901 atgttcgacg aatggcgctc gggacgagcg ccgctacctt tgcgacctac tccgcgcgga
      961 gcgccgtgag cgggtcgact ttggtcgcgc gacgagcggg gacgaaggac gcgatgacgg
     1021 cgacgacgaa gaggagaatg gcggcggcga tgagtccgac cggatcggcc atggcgagcc
     1081 gcggcatgaa cgaggcgaga aatcggccga ggccccaggc acccgcggcg ccgatcacaa
     1141 ggccgagcgc ggtgagcgcg aggacgcgtt
//
LOCUS       DASPLT010001066         1499 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_177317, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001066 DASPLT010000000
VERSION     DASPLT010001066.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1499)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1499)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1499
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_177317"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            309..800
                     /locus_tag="VEA63_11005"
     CDS             309..800
                     /locus_tag="VEA63_11005"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HYD84577.1"
                     /translation="MKHRSFAPPLALCVGLCLSTATFAAQEKPTEARAQPEYFAPPST
                     GGTPLPFSEAVKFGDTLYVSGQIGNLPGTLTLAPGGIEAEARRTLDNMKAILERHGSS
                     MEQVVKCTVFLADIKEWPVFNAIYREYFKTNLPARSALGANGLALGARVEVECIAFVP
                     PKK"
     gene            complement(989..>1499)
                     /locus_tag="VEA63_11010"
     CDS             complement(989..>1499)
                     /locus_tag="VEA63_11010"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HYD84578.1"
                     /translation="TSFYLPPEKADRLANVYGIENGKLVLKENAANSLYVHGPRKCFS
                     GGAGLLSTASDYGRFLQMLLNGGELDGVRLLSPKTVELMHANHTGDKFGRDVSAFGLG
                     FWVNQDPGYYGELSSEGAYGWGSAYFPQYVVDPKERLVAVFMTQLLPTGGLNLNQTFK
                     VLMYQALVK"
BASE COUNT          288 a          602 c          352 g          257 t
ORIGIN      
        1 tccggttcaa ctcagcggcc agtgtcgctt cagtgtcagt atcgcttggc cgccgttctc
       61 gacacgcact gccacaagca tttcccccgt aatccgccac gggattaaac ctgatcccca
      121 actcgcagcc tcggcgacgg cccgcacgcc gcacaaaacc cccgccgcct cgcgccggct
      181 ctgccaacac aacacaccca accgcacacg cacgaccacc gcgccactcc acctccggcg
      241 aatccacgct cgcctcccgc gctggcccat cgttccaatt caccgctccc aaaccctttc
      301 catcgcttat gaaacaccgc tccttcgccc ctcccctcgc actttgtgtc ggcctttgcc
      361 tttccaccgc gacgttcgcc gctcaggaaa aaccgaccga agctcgcgcg cagccggagt
      421 atttcgcgcc gccatcgacc ggcggaacgc cattgccctt ctccgaagcc gtcaaattcg
      481 gcgatacgct ttatgtctcc ggccagatcg gcaacctacc gggcaccctc acgctcgcgc
      541 cgggcggcat cgaagcggaa gcacgccgca cgttggacaa catgaaagcc atcctcgagc
      601 gacacggcag ctcgatggag caggtcgtca agtgcacagt ctttctcgcc gacatcaaag
      661 agtggcccgt gttcaacgcg atctaccgcg agtatttcaa aaccaacctc ccggcacgca
      721 gcgcgctcgg cgccaacggc ctcgccctcg gcgcccgcgt cgaggtcgaa tgcatcgcct
      781 tcgtcccgcc gaaaaaataa ctcgccgacc gccccaccgt cctccccgac aaccccaaca
      841 ggtagggacg cctctccgag gcgtccgcca ctatgccccc atccgccacc ccgcccgcat
      901 ccgtccccgc cgatcgcgtt ccgcagcgac gctcgagccc aacccaccgc gagcgcgcca
      961 ccgccggcgc tctcgcgccg ccggcgcgct acttcacgag cgcctgatac atcagcacct
     1021 tgaacgtctg gttcaagttg agcccgcccg tcggcagtag ttgcgtcatg aataccgcca
     1081 ccagccgctc tttcgggtcc acgacatact gcgggaaata cgcgctgccc catccatacg
     1141 cgccttcgct gctcaactcg ccgtagtatc caggatcttg attcacccag aacccgagcc
     1201 caaacgcgct cacgtcgcgc ccgaatttgt cgcccgtgtg gttcgcatgc atcaactcaa
     1261 ccgtcttcgg actcagcaaa cgcacgccgt ccagctcgcc gccgttcagc agcatctgga
     1321 gaaaccgccc gtagtcagac gccgtcgaca agagccccgc cccacccgag aaacactttc
     1381 tcggtccatg cacgtaaaga ctgttcgccg cgttctcctt cagcacgagt ttcccattct
     1441 cgatcccata cacgttcgcc aggcggtccg ccttttccgg cggtagatag aaactcgtg
//
LOCUS       DASPLT010001067         2146 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_155838, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001067 DASPLT010000000
VERSION     DASPLT010001067.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 2146)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2146)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2146
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_155838"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..843
                     /locus_tag="VEA63_11015"
     CDS             <1..843
                     /locus_tag="VEA63_11015"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HYD84579.1"
                     /translation="EVLQEIDEGRLSIEAIILRETGHELPPGLSQRLVELYFGGVPTY
                     TLELFHQVYWRKIPLYRLNQTWLFQEGFRIAREPVFERLKRVSDIVFALTGLVLASPF
                     ILLGALAILIDDGGPVFFSQNRVGRNHRPFKIRKLRTMRSSPPPGASTPPIPGANELY
                     TQLNDARITRVGRFLRATRIDEFPQLWNVLVGDMSLIGPRAEWDRLVQNYEREIPCYY
                     FRHLVKPGITGWAQVNYPYGASIDDTLRKLEYDLYYIRHFSFLLDASIMLKTIHIMLF
                     GKGR"
     gene            859..>2146
                     /locus_tag="VEA63_11020"
     CDS             859..>2146
                     /locus_tag="VEA63_11020"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HYD84580.1"
                     /translation="MKSYDFLVLGGGSAGFNAARVATSLGLKTAIVDGARELGGLCIL
                     RGCMPSKTLLYMAEVLHLAQKGRTFGLNIPSARPDMKAMHARKKKIIADFASYRVQAL
                     ERGDFDLIRANARFLDPHTVELSDGRKLRSKYFLIGTGSKPSVPAVPGLAETPFWTSD
                     DVLDLDFVPKSVIILGGGIVACELAQFLTRIGTRVILLQRSINILRDHSAHASQVIQQ
                     ALQDEGVELFAGTHLQRVWSDRRGVNVEFLCAGKVTTRRAAHLFNALGREPNTASLNL
                     RAAGVDTRITGQVVINRWQQTRAPHIYAAGDCSGPAEIVHVAIQQGELAARHAAGVKK
                     IQPVDYSLLLNVVFTDPQLATIGALERELDHKKRKYLVASYPFNDHGKSILMEANYGY
                     VKVIAEPRRGRILGAEIVGKDAGELVHAFSGPLAMRA"
BASE COUNT          399 a          784 c          573 g          390 t
ORIGIN      
        1 gaggtgctgc aggagatcga cgagggccgc ttgtccatcg aagcgattat tttgcgcgaa
       61 accggacacg agctccctcc cggcctttcg cagcgactgg tcgaactgta ttttggcggc
      121 gtgccgacct acacgttgga gctgtttcac caggtttact ggcgaaagat cccgctctac
      181 cggctcaacc aaacctggct cttccaagag ggcttccgca tcgcgcgcga gccggtgttc
      241 gagcgtctga agcgggtctc cgacatcgtt ttcgcgctca ccggcctcgt cctcgcatcg
      301 cctttcatcc tgctcggcgc gctcgccatc ctgatcgacg acggcggacc cgttttcttt
      361 tcgcaaaacc gcgtcggccg gaatcaccgc cccttcaaga tacgcaagct ccgcaccatg
      421 cggtcgagcc ctcctcccgg cgcgagcacc ccgcccattc ccggcgccaa cgaactttac
      481 acgcagctca acgacgcgcg catcactcgc gtcggccgct tccttcgcgc cacgcgtatc
      541 gacgaatttc cccagctttg gaacgtgctc gtcggtgaca tgagcctcat cggcccgcgc
      601 gccgaatggg accggctcgt gcaaaattac gaacgcgaga tcccgtgcta ctacttccgc
      661 cacctcgtaa aacccggcat cactggctgg gcgcaagtca actatcccta cggcgcgagc
      721 atcgacgaca cgttgcgcaa attggaatat gatctgtatt acatcagaca tttttccttc
      781 ctgctcgatg cctcaatcat gctgaagacg attcacatca tgctgttcgg caaaggccgc
      841 taaaccgccg aattcaccgt gaaaagctac gattttcttg tcctcggcgg cggcagcgcc
      901 ggcttcaacg ccgcacgcgt cgcgacgtcg ctcggtctca aaaccgccat cgtcgacggc
      961 gcgcgcgagc tcggcggcct ctgcattctc cgcggctgca tgccgtcgaa gacgctgctc
     1021 tacatggccg aggtgctcca cctcgcgcag aagggccgga cgttcggcct gaacattccg
     1081 tcggcccgcc ccgacatgaa agcgatgcac gcgcgaaaga aaaaaatcat cgccgacttc
     1141 gccagctatc gcgtgcaagc gctcgaacgc ggcgacttcg atctcattcg cgccaacgcc
     1201 cgcttcctcg atccccacac cgtcgaactc tccgacggcc gcaaactccg ctccaaatat
     1261 tttctcatcg gcaccggctc gaagcccagc gtgcccgccg tgcccggtct cgccgaaacg
     1321 cctttttgga cgagcgacga cgtgctcgac ctcgatttcg tgccgaagag cgtgattatc
     1381 ctcggcggcg gcatcgtcgc ctgcgagctc gcgcaatttc tcactcgcat cggcacgcgc
     1441 gtgattctgc tccaacgcag catcaacatt ctccgcgacc actccgcgca cgcgtcgcag
     1501 gtgattcagc aagcgctcca ggacgagggc gtggagcttt ttgccggcac gcacctccag
     1561 cgcgtctggt ccgaccgccg cggcgtcaac gtcgagttcc tctgtgcggg caaggtgacc
     1621 acccgccgcg ccgcgcacct gttcaacgcc ctcggccgcg agcccaacac cgcctcgctc
     1681 aacctccgcg ccgccggcgt cgacacccgc atcaccggcc aggtcgtgat caatcgctgg
     1741 cagcaaaccc gcgcgccgca catctacgcc gccggcgatt gctccggccc ggcggagatc
     1801 gtccacgtcg ccatccagca gggcgaactc gccgcacgcc acgccgctgg cgtgaaaaaa
     1861 atccaacccg tcgactactc gctcctcctc aacgtggtgt tcaccgatcc gcagctcgcg
     1921 accatcggcg cgctcgaacg cgagctcgat cacaagaaac ggaaatacct cgtcgcctcg
     1981 tatccattta acgatcacgg caagtcgatc ctcatggagg ccaactacgg ctacgtgaaa
     2041 gtcatcgccg agccccgccg cggccgcatc ctcggcgccg agatcgtggg caaggacgcc
     2101 ggcgaattgg tccacgcctt cagcggcccg ctggccatgc gagcca
//
LOCUS       DASPLT010001068         2432 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_114157, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001068 DASPLT010000000
VERSION     DASPLT010001068.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 2432)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2432)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2432
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_114157"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..431
                     /locus_tag="VEA63_11025"
     CDS             <1..431
                     /locus_tag="VEA63_11025"
                     /inference="COORDINATES: protein motif:HMM:NF014376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HYD84581.1"
                     /translation="ATLAFAAGVAVEQNRPPELSSRIYRKADARNSSGDWGSIHIHTP
                     ETASSAGLASLLTAELEFLPGKQFQPPHQHPEEELQYIVEGGGTWFLNGENIPIEKGD
                     LMYAKPGDLHGISNTTDAPLRFLVVKWSGKHAADTRSSAP"
     gene            513..1430
                     /locus_tag="VEA63_11030"
     CDS             513..1430
                     /locus_tag="VEA63_11030"
                     /inference="COORDINATES: protein motif:HMM:NF020039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HYD84582.1"
                     /translation="MKTPLLLRLIATAVAPFVIASSFAADTPFRSIAVGATPESVTPG
                     FGGKLHVTLMGAKREKGDSDGKIVVVDGDNVTVLAEGFDDPKGLVFIDDRLVTADFDK
                     IWAIDASGAKRLIAGPDAFPTPPLYLNDLVVEPDAGSILVTDMGNLAAMHASPGVFWP
                     LDSEQAKTIQPLGRVYRVTLDGKVSLVIDHAVEMPNPNGIDRLADGTLLVAEFFRGTL
                     LEWRSGTWRTISDDHRSGDGVAHDAAGNLYVSEVRTGRVWHVKYPSGEKRLLATLQSA
                     ADLYLDEPQAQLVVPDSKAGTLVFIPLAN"
     gene            complement(1657..>2432)
                     /locus_tag="VEA63_11035"
     CDS             complement(1657..>2432)
                     /locus_tag="VEA63_11035"
                     /inference="COORDINATES: protein motif:HMM:NF018178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HYD84583.1"
                     /translation="SQWDTYLSFQHAPNYNGQEPVDSEGRKIEPVGYGRDPRGVFSVI
                     EQDGQLVLHVSGEIYGCVFTRQEFENYRLRLKVKWGEKKWVPRQDKLRDSGILYHSIG
                     PAGAEYWRSWMLSQEFQIMEGHMGDYWSQASSGIDIRAFLPEGAMNSVASETQPFLPF
                     GAKPSVSGFCLRSADHESRPGEWTELELVCFDGKSLHLVNGEVVMVLKDSRYVKDGRA
                     IPLTKGKIQLQSEAAEVFFTDIEIKPIDAMPPEFEALFR"
BASE COUNT          489 a          857 c          619 g          467 t
ORIGIN      
        1 ccgcgacgct cgcgttcgcc gccggcgtgg ccgtcgaaca aaatcgcccg cccgaattga
       61 gcagccgcat ctaccgcaaa gccgacgccc gcaactcctc gggcgattgg ggttcgattc
      121 acattcacac ccccgaaacc gcatcgagcg ccggcctcgc gagcctcctc accgcggaac
      181 tcgaatttct tcccggcaaa cagttccagc cgccgcacca acatccagag gaagaacttc
      241 aatacatcgt cgaaggcggc ggcacgtggt ttctcaacgg agagaacatc cccatcgaaa
      301 agggagacct catgtatgcc aaacccggcg acctgcacgg catctccaac accaccgacg
      361 cgcccctgcg ttttctcgtc gtgaaatggt ccggcaaaca cgccgccgac actcgctcgt
      421 cagctccttg agattcacat cccaagcgtc tccggctgaa gtaggctcac gtttcattcc
      481 ttcatcgcac ccaatcccca acccagctcc ttgtgaaaac acccctgctc cttcgcctca
      541 tcgccaccgc ggtcgccccc ttcgtcatcg cgagcagctt cgccgccgac acgccttttc
      601 gctcgatcgc cgtcggtgca acgcccgaga gcgtcacacc cggcttcgga ggaaaactcc
      661 acgtcaccct catgggcgcg aaacgcgaga aaggcgacag cgacggaaaa attgtcgtgg
      721 tcgacggcga caacgtcacc gtgctcgccg aaggtttcga cgaccccaaa gggctcgtgt
      781 tcatcgacga tcggctcgtc accgccgact tcgataaaat ctgggcgatc gacgcgagcg
      841 gcgcgaagcg gctcatcgcc ggccccgacg cgttccccac gcctccgctt tacctcaacg
      901 atctcgtcgt cgagccggat gccggcagta tcctcgttac ggatatgggc aacctcgctg
      961 cgatgcacgc gagccccggc gttttctggc cgctcgatag cgagcaggcg aaaacgatcc
     1021 agccgctcgg tcgcgtttac cgcgtcacgc tcgacggaaa agtgtcactc gtgatcgatc
     1081 acgccgtcga aatgcccaac cccaatggca tcgaccgact ggcggacggc acgctcctcg
     1141 tcgcggaatt ctttcgaggc acgctgctcg aatggagatc aggcacatgg cgaacgatca
     1201 gcgacgatca ccgcagcggc gacggcgtcg cgcacgatgc cgcgggcaat ctctacgtct
     1261 ccgaagttcg caccggccgg gtgtggcacg tcaaataccc ctcgggcgaa aagcgcctgc
     1321 tcgccacgct ccaatccgcc gccgaccttt atctcgacga gccccaagcc caactcgtcg
     1381 tccccgattc aaaagccggc acgctcgtct tcatcccgtt ggcgaactga cgagcggtgg
     1441 taatcgtcgc tccgatcagc cacgtcgttt caattctagc gggcagctaa ccgctcatca
     1501 tcgctaatgg catcgtcgaa ttcccacgag acgtccgagc ttcgccgatc aggcgaagct
     1561 cgcagccatt cgctctcaca accgtcctcc gaaaaatcag cgaagattag cggttcaaaa
     1621 ctttgtcttc ggcgtccggc ttgccggcca ggatctctat ctgaacaacg cctcgaactc
     1681 cggcggcatc gcatcgatcg gtttgatttc gatgtcggtg aaaaacactt cggccgcttc
     1741 gctctggagt tggatttttc ctttcgtcag cgggatcgcg cggccgtctt tcacgtagcg
     1801 cgaatccttc agcaccatca cgacctcgcc gttgacgagg tgcaggcttt taccgtcgaa
     1861 acaaacgagc tccaattccg tccactcgcc cggcctgctc tcgtgatccg cgctgcgcag
     1921 gcagaaaccg ctcacgctcg gcttcgcgcc aaacggcaga aacggctgcg tctcgctcgc
     1981 cacgctgttc atcgcacctt ccggaaggaa cgcacggata tcgatccccg agctcgcctg
     2041 cgaccaatag tcgcccatgt gcccttccat gatctggaat tcctgcgaaa gcatccacga
     2101 ccgccagtat tccgcgcccg ccggcccgat cgagtgatac aggatgccgg aatcgcgcag
     2161 tttgtcctgc cgtggcaccc acttcttttc gccccatttc actttcagtc tcaggcgata
     2221 attctcgaac tcctgccgcg tgaacacgca cccgtaaatt tccccgctca cgtgcagcac
     2281 gagctgtccg tcctgttcga tgacggagaa cacgccgcga ggatcgcgcc catagccaac
     2341 cggctcgatt ttccttcctt ccgagtcgac cggctcttgt ccgttgtaat tcggagcgtg
     2401 ttgaaaactc aaatacgtgt cccattgcga aa
//
LOCUS       DASPLT010001069         1036 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_52409, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001069 DASPLT010000000
VERSION     DASPLT010001069.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1036)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1036)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1036
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_52409"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            229..915
                     /locus_tag="VEA63_11040"
     CDS             229..915
                     /locus_tag="VEA63_11040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD84584.1"
                     /translation="MKKARLHFPRIRPIAYEGTKTTNALAFRHYNPEEVVDGKSMEAH
                     MRFSIAYWHAFRGVGSDPFGPGTITRPWEKGNNELSIALTRMDAAFEFFQKIRAPFWA
                     FHDRDIAPEGRSLAESNRLLDRVVAHAKDLQKETGVKLLWGTANLFSNPRYMSGASTN
                     PDAHVFAYAAAQVKKAMDVTKALGGENYVFWGGREGYETLLNTNLKREQDHLAAFLHM
                     AVDYAKEIGF"
BASE COUNT          209 a          318 c          309 g          200 t
ORIGIN      
        1 tcgcctgcaa gcaggctcct acctccggtc cgatgaagat tgtgcgcgag acgttcttgc
       61 ggagcagcgg cccaccggcg cggttcgccc tgcgcatcgt gcgccaaacc ggcggttagc
      121 agcctgcacg aatgtgagac gaacgccgcc gcatcggtcg ctttatttcg cttcccattg
      181 cgacgacggc cggttttatg gccgtgctct atttatagaa tacccgccat gaagaaagct
      241 cgcctgcact tcccccgcat tcgcccgatc gcctacgagg gcaccaagac gacgaacgcg
      301 ctcgcgtttc gccattacaa ccccgaggag gtcgtcgacg gcaagtcgat ggaagcgcac
      361 atgcgcttct cgatcgctta ctggcacgcg tttcgcggcg tcggttccga tccgttcggc
      421 ccgggcacga tcacgcgtcc atgggaaaag ggtaacaacg agctgtcgat cgcgctgacg
      481 cgcatggatg cggcgttcga gttcttccaa aaaatccgcg cgccgttctg ggcgtttcac
      541 gatcgcgaca tcgctcccga gggacgttcg cttgcggaat cgaaccggtt gctcgatcgc
      601 gtcgtcgcgc acgcgaagga cctgcagaag gaaacgggcg tgaagctcct ctggggcacg
      661 gcgaacttgt tttcgaatcc gcgctacatg tcgggtgcgt cgacaaatcc cgacgcgcat
      721 gtgttcgcct atgccgcggc gcaggtgaaa aaagcgatgg acgtcacgaa agcgctcggc
      781 ggcgagaact acgttttctg gggcggtcgc gaaggctacg agacgctcct caacacgaac
      841 ttgaagcgcg agcaggacca cctcgcggcg tttcttcaca tggctgtcga ttacgcgaag
      901 gagatcggct tctagggcca attcctcatc gagccgaagc cgaaggagcc gacgaagcac
      961 caatacgatt tcgatgtcgc gagcgggatc gcgtttctcc gcacctacgg gctcgagaaa
     1021 tatttcaagt tcaaca
//
LOCUS       DASPLT010001070         1196 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_36610, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001070 DASPLT010000000
VERSION     DASPLT010001070.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1196)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1196)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1196
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_36610"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..500
                     /locus_tag="VEA63_11045"
     CDS             <1..500
                     /locus_tag="VEA63_11045"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HYD84585.1"
                     /translation="AEMATRHGRRDEAESLLVRALAIDDRDLDAHRMLGRLRWDVARR
                     DEAFAHFERVVQLDPTELPLRVLVGQHQLERGEPASALAPLTEAAALAPDDADVRHLL
                     ELAWLRHGNRQAREREFDKALTSYEEALRIEPGNVEARTNRVRVLAHVRRLDESGNAA
                     NPRSE"
     gene            631..>1196
                     /locus_tag="VEA63_11050"
     CDS             631..>1196
                     /locus_tag="VEA63_11050"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HYD84586.1"
                     /translation="MNRAILIFAVALAIAALVWLARNHALDRAGDAHAALPHSADSSA
                     PSVLRSGSFTPRPIGTVPAANQPAWITDLTVADLDDDGFPDVIACDARTNRIVWIRQV
                     ARGEYLEQSIGAAIAGPAHVEAVDFDGDGDLDLLIAAMGIVPPSTERTGSVAILENDG
                     RENFTTRVILERTHRVTDVSAGDLNGDGR"
BASE COUNT          198 a          374 c          417 g          207 t
ORIGIN      
        1 tggccgagat ggcgacgcgt cacggccggc gcgatgaagc cgaatcgctg ctggtgcgcg
       61 cgctcgcgat tgacgatcgg gatctcgacg cgcaccgcat gctcggccgt ttgcgatggg
      121 atgtcgcgcg gcgcgacgaa gcgttcgccc acttcgaacg ggtggtgcaa ctcgatccga
      181 ccgagcttcc gctgcgcgtg ttggtcggcc agcaccagct cgaacgcggc gaacctgcgt
      241 cagcactcgc gccgctcacc gaagccgccg cgctggcgcc cgacgatgcg gatgttcgtc
      301 accttctcga gctcgcgtgg ttgcggcacg gcaatcgaca ggcgcgcgag cgtgagttcg
      361 acaaggcgct gacgagctat gaggaagcgc ttcgcatcga gccgggaaac gtcgaagccc
      421 gcacgaatcg ggttcgcgtg ctggcacatg tccgccgact cgacgaatcg ggtaacgctg
      481 cgaacccgcg ctccgagtga aaccgcccta ttcagacgcg cacggttatg ggagccggtt
      541 tgcgggcgat ggagccatcc cgctgctcac gcagcgagcc acgcgggagg cactcgatcg
      601 cttgcgagca agctcctccc tctcgatccg atgaatcgtg cgattttgat cttcgctgtt
      661 gcgctcgcga ttgccgcgct ggtgtggctc gcacgcaacc atgcactcga ccgcgcgggc
      721 gatgcgcacg cggcactccc tcacagcgcg gactcgtccg ctccgagcgt gttgcgcagc
      781 ggctctttca cgccgcggcc gatcggcacg gttcccgcgg cgaatcagcc ggcgtggatc
      841 accgatctga cggtggccga tctcgatgac gacgggtttc ccgatgtgat cgcatgcgat
      901 gcgcgaacga atcggatcgt gtggattcgg caggtcgcgc gcggggaata tctcgaacag
      961 tcgatcggag ctgcgatcgc gggaccggcg cacgtggagg ccgtggattt cgatggcgac
     1021 ggcgatttgg atttgttgat cgcggcgatg ggaatcgtgc cgccgagcac ggagcgcacg
     1081 ggctcggtgg cgattttgga gaacgacggg cgggaaaatt tcacgacgcg cgtgattctc
     1141 gagcgcacgc accgtgtgac cgatgtgagc gcgggcgatc tcaacggcga cgggcg
//
LOCUS       DASPLT010001071         1010 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_114215, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001071 DASPLT010000000
VERSION     DASPLT010001071.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1010)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1010)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1010
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_114215"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(93..>1010)
                     /locus_tag="VEA63_11055"
     CDS             complement(93..>1010)
                     /locus_tag="VEA63_11055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009513220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HYD84587.1"
                     /translation="LRMNKIRITAYHVVFFIFVISNVGGALTPIGDPPLFLGFLRGVP
                     FFWLLERVMVEWLATLGAILAVFYVIDRRYYKRMPVAVQREVEAPDTWRFEGGANLLL
                     LLVIIGAVFLPATFFVREIVMIGAAVASYWLTPREVHTKNQFGFGPIKEVAFLFLGIF
                     ATMMPALNYLDAHGQEFGLKKPAQYYFATGSLSSVLDNAPTYLNFLQLAKATALAEEP
                     ELFVAAGSDERAQVGVLLERFPKLVIAVSLGAVFFGAMTYIGNGPNFMVKSIAESAGV
                     KVPTFFGYIFKYSLPILLPILALGGWWFL"
BASE COUNT          250 a          360 c          240 g          160 t
ORIGIN      
        1 tgaagccccg gatcactccg accgctggag cgaaccgatt ccggtgatct gcgcccggcg
       61 agcaaaactc gcccaccttc aggctgtctc agctacaaaa accaccatcc gcccagcgcg
      121 agaatcggca gtaaaatcgg caggctgtat ttgaaaatat agccgaaaaa cgtcggcacc
      181 tttacgcccg cgctctccgc gatcgatttc accatgaagt tcggcccgtt gccgatataa
      241 gtcatcgcgc cgaaaaacac cgcgccgaga ctcaccgcga tcaccagctt cggaaaccgc
      301 tcgagcaaca cccccacctg cgcccgctcg tcgcttcccg ccgccacaaa caactccggc
      361 tcctccgcca gcgccgtcgc ttttgcgagc tggaggaaat tcaaataggt cggcgcatta
      421 tccagcaccg acgacagcga gccggtggcg aaataatact gcgccggctt cttcaatccg
      481 aattcctgcc cgtgcgcgtc gagatagttc agcgccggca tcattgtcgc gaaaatcccg
      541 agaaacagga acgcgacctc cttaatcggt ccgaacccaa actggttctt tgtgtgcacc
      601 tcgcgcggcg tcagccaata cgaagccacc gccgcgccga tcatcacgat ctcccgcacg
      661 aagaaggtcg ccggcaaaaa caccgcgcca atgatcacca acagcagcaa caggttcgcg
      721 ccgccttcga accgccacgt gtccggcgcc tccacctcgc gctgcaccgc caccggcatc
      781 cgtttataat aacgccggtc gatcacgtag aacaccgcca gaatcgcacc gagcgtcgcc
      841 agccactcga ccatcacccg ttcgagcagc cagaaaaacg gcacaccgcg cagaaaccct
      901 agaaacaacg gcggatcgcc aatcggcgtc agcgcaccgc ccacgttcga aatcacaaag
      961 atgaaaaaca ccacgtgata tgcggtgatc cgaatcttgt tcatccgcag
//
LOCUS       DASPLT010001072         1910 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_93816, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001072 DASPLT010000000
VERSION     DASPLT010001072.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1910)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1910)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1910
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_93816"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..792
                     /gene="rpoN"
                     /locus_tag="VEA63_11060"
     CDS             <1..792
                     /gene="rpoN"
                     /locus_tag="VEA63_11060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HYD84588.1"
                     /translation="AARMLRDHFELLSRRRIPELSRKLGVAPDDVQAAIEEIGKLDPA
                     PGRRFAEDNNRVVVPDVVVEKDGDEWKIHLNSDYIPRLRISSTYRELIAKGTLSKDER
                     DYLRERMRSGKFLIDSIEQRQRTIERITREIIKAQTPFFENGVAHLRPLTMTQIADIV
                     GVHETTVSRAIANKYMKTPHGVFDFKYFFTTGYQADSGASVSNTSVKEMLADLFTMED
                     KSSPLSDQELVAKLAEKGLTLARRTVAKYREELGILPSNLRRDYK"
     gene            complement(853..>1910)
                     /gene="tsaD"
                     /locus_tag="VEA63_11065"
     CDS             complement(853..>1910)
                     /gene="tsaD"
                     /locus_tag="VEA63_11065"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HYD84589.1"
                     /translation="PSPRADMILALESSCDETAVAVFDPQRGILGEWVHSQIALHERH
                     GGVVPDLATREHLRTFGPLLERARTAVEFSGISEVAVTNGPGLAACLAMGVAAAKAVA
                     LQLRVPVAGVNHLRGHVWSPFIALHAAAPERFDERMSELLPHLGLIVSGGNTLLFALD
                     AGRKVTVLSSTRDDAAGEALDKGAKLLGLGYPGGPLIEKLAKSGRADAYEFPRGIGRR
                     DELDFSFSGVKTSLRYLIEKLTPEDVQLRLPDLCASYQQAVIDALVRKTRAALRLAEG
                     RYRSVGSSGGVANNATLRAALERAAKHERIPFFAAEPRHTGDNAAMIAFAAWADWTGV
                     ERGEKMALRVEPSAALA"
BASE COUNT          374 a          714 c          497 g          325 t
ORIGIN      
        1 gccgcccgca tgttgcgcga tcattttgaa ctgctctcgc gtcgtcgcat ccccgagctt
       61 tcacgcaagc tcggcgtggc gcctgacgac gtgcaggccg ccatcgagga aatcggcaaa
      121 ctcgatcccg cgcccggccg ccgtttcgcc gaagacaaca accgcgtggt cgtgcccgat
      181 gttgtcgtcg aaaaagacgg cgacgagtgg aagattcacc tcaacagcga ctacatcccg
      241 cggcttcgca tctcaagcac ctaccgcgag ctcatcgcca aaggcacact gtcgaaagac
      301 gagcgcgact acctccgcga gcggatgcgc tccggcaagt ttctcatcga ttcgattgag
      361 caacgtcagc gcaccatcga acgcatcacg cgcgagatca tcaaagcgca gacgcctttc
      421 ttcgaaaacg gcgtcgctca tctgcgcccg ctcacgatga cccaaatcgc cgacatcgtc
      481 ggcgtgcacg agacgaccgt cagccgcgcg atcgcgaaca aatacatgaa gaccccgcat
      541 ggcgtcttcg atttcaaata tttcttcacg accggttatc aggccgacag cggcgcctcc
      601 gtttcgaaca ccagcgtgaa agagatgctc gccgacctct tcacgatgga ggacaaatcc
      661 tcgcccttga gcgatcagga actcgtcgcg aaactcgccg aaaaaggtct cacgctcgcc
      721 cgtcgcaccg tcgccaaata ccgcgaagaa ctcggcatcc tcccgagcaa tctccgccgc
      781 gattacaagt agcccttttg tagggcgggg tctcccgacc ccgccgctcc ggttgcctga
      841 cgccgcttcc cgctatgcca gcgccgcgct cggctccacc cgcagcgcca tcttttcgcc
      901 gcgctccaca cccgtccagt ccgcccacgc cgcgaacgcg atcatggccg cgttatcgcc
      961 cgtgtgcctc ggctccgccg caaaaaaagg aattcgctcg tgcttcgccg cacgctcgag
     1021 cgccgctcgc agcgtcgcgt tgttcgccac accgcccgac gacccaacgc tccgataacg
     1081 tccctccgcc agccgtagcg ccgcgcgcgt ttttctgacg agcgcatcga tcacggcctg
     1141 ctggtaactc gcgcaaagat ccggcaggcg caactgcacg tcctctggcg taagtttttc
     1201 gatcagataa cgcaggctgg ttttcacacc cgaaaaactg aaatccagtt catcgcgccg
     1261 cccgatgcct cgcggaaact cgtaggcatc cgcgcgcccg cttttcgcga gcttctcgat
     1321 cagcggtccc ccgggataac caagcccgag cagcttcgcg cccttgtcca acgcctcgcc
     1381 cgccgcgtcg tcgcgcgtcg acgacaacac cgtcaccttg cgtcccgcat cgagcgcaaa
     1441 caagagcgtg tttccgccgg aaacaatcag ccccaaatga ggcagcagtt cgctcatccg
     1501 ctcgtcgaag cgctccggcg ccgccgcatg cagcgcgata aaaggcgacc acacgtgacc
     1561 gcgcagatga ttcacgcccg ccacgggcac ccgcagttgt aacgcgaccg ccttcgccgc
     1621 tgccacgccc atcgccaggc acgccgccaa tcccggccca ttcgtcaccg ctacctcgct
     1681 gatgccgcta aactccaccg cggtccgcgc tcgctcgagc agcggtccaa acgttcgcag
     1741 gtgctcccgc gtcgccagat cgggcaccac cccgccgtgc cgttcgtgca acgcgatctg
     1801 gctatgcacc cattcgccta aaatcccgcg ctgcggatcg aacaccgcca ccgctgtctc
     1861 gtcgcaagaa ctctcgagcg ccaggatcat gtcagcgcgc ggcgatggtt
//
LOCUS       DASPLT010001073         2290 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_156032, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001073 DASPLT010000000
VERSION     DASPLT010001073.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 2290)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2290)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2290
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_156032"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..881)
                     /locus_tag="VEA63_11070"
     CDS             complement(<1..881)
                     /locus_tag="VEA63_11070"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016813 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear
                     amidines [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/carbamoylase family amidase"
                     /protein_id="HYD84590.1"
                     /translation="MKRDSMASLERVLARRLDELGGVSEEKDALTRTFLSAAMGQAHG
                     LVGRWMMEAGLEVSVDATGNLIGRMEAAAPGAKTLLLGSHLDTVRNAGRFDGALGVVL
                     PIVALAELRRRGVRLPFAVEVIGFSEEEVVRFPCAYIGSKGYAGRLRASDLKVRDGAG
                     ASLREALETFNQGAFALPKPAHAARELLGYAEVHIEQGPVLEDEGLAVGVVSAIASQV
                     RGKLTFRGRAGHAGTTPMRLRRDALAGAAEFILLAEEFARAREPLVATVGTIEVPRSA
                     TNVIPGAAVVSLDVRHPR"
     gene            complement(887..1231)
                     /gene="uraH"
                     /locus_tag="VEA63_11075"
     CDS             complement(887..1231)
                     /gene="uraH"
                     /locus_tag="VEA63_11075"
                     /EC_number="3.5.2.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR02962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019628 - urate catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyisourate hydrolase"
                     /protein_id="HYD84591.1"
                     /translation="MPAKLSTHVLDLTAGRPAAGMGIQLWRLGERPARLRTVMTNADG
                     RTDAPLLGAEEMAVGTYELVFDVKSYFAGRGVECVFLGDVPVRFAITNAGASYHVPLL
                     VTPWSYSTYRGS"
     gene            complement(1307..1855)
                     /gene="uraD"
                     /locus_tag="VEA63_11080"
     CDS             complement(1307..1855)
                     /gene="uraD"
                     /locus_tag="VEA63_11080"
                     /EC_number="4.1.1.97"
                     /inference="COORDINATES: protein motif:HMM:NF020907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019628 - urate catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline
                     decarboxylase"
                     /protein_id="HYD84592.1"
                     /translation="MMTLAQLNQLDRAAFTAALGHLFEHSPWVAEEAWAKRPFRDTAA
                     LHAELCETMRAAPKEKQLELIRAHPDLAGRMAQEKKLTAESAREQASAGLDQLSDEEL
                     ANFQRLNAEYRERFGFPFVICARLNARDAILRAMRERLKNTPEAEHAAALREIEKIAW
                     LRLQDAVNQAPSTKFQAPEKNQ"
     gene            complement(1988..>2290)
                     /locus_tag="VEA63_11085"
     CDS             complement(1988..>2290)
                     /locus_tag="VEA63_11085"
                     /inference="COORDINATES: protein motif:HMM:NF044584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD84593.1"
                     /translation="GGSGAEPIHYLMEDLATSEISRSQLWQWLKFGAKLDDGRAITVE
                     FYETLLADELVKIRGEYGAGVYDAGHFKEATALFMRMVTSETFDEFLSLPAYELLA"
BASE COUNT          453 a          902 c          533 g          402 t
ORIGIN      
        1 cgcggatgcc gcacatcgag cgacacgacc gccgcccccg ggatcacatt cgtcgcactc
       61 cgcggcacct cgatcgttcc caccgtcgcg acgagcggct cgcgcgcccg cgcgaattcc
      121 tccgccaaca aaataaactc cgccgcgccc gccagcgcat cgcgccgcaa tcgcatcggc
      181 gttgtccccg catgccccgc ccgcccgcga aaggtcagct ttccgcgcac ctggctcgcg
      241 atcgccgaca ccacgcccac cgccagtcct tcatcttcca acaccggccc ctgctcaatg
      301 tgaacctccg cataacctaa aagctcgcgc gcggcatgcg ccggcttcgg aagcgcgaac
      361 gctccctggt tgaatgtttc cagcgcttca cgcagcgacg cgccggcgcc atcgcgcacc
      421 ttcaaatcgc tcgcccgcaa acgccccgcg taacctttgc tgccgatgta agcgcacgga
      481 aatcgcacga cttcctcctc cgaaaatcca atcacctcca ccgcgaaagg cagccgcacg
      541 ccgcgccgcc gcaactccgc caatgccacg atcggcaaca ccacgccgag cgcgccgtca
      601 aatcgccccg cgtttcgcac cgtatccaga tgcgagccca gcagcagcgt cttcgcgcca
      661 ggcgccgctg cttccatccg gccaatcaaa tttcccgtcg catccacgct cacctccaac
      721 cctgcctcca tcatccagcg cccgaccaat ccatgcgcct gtcccatcgc cgccgacaga
      781 aacgtccgcg tgagcgcgtc cttctcctcg gacacaccgc cgagttcgtc gagccgtcgc
      841 gccagcactc gttcgagcga agccatcgaa tcgcgtttca tctcgatcag ctcccgcgat
      901 acgtgctgta gctccacggc gtcaccagca gcggcacgtg ataactcgcg cccgcattcg
      961 tgatcgcaaa acgcaccggc acgtcgccga gaaaaacgca ctccacgccg cgccccgcaa
     1021 aatacgactt cacgtcgaac accaattcgt aagtccccac cgccatctcc tccgcgccga
     1081 gcaacggcgc gtccgtccgc ccgtccgcat tcgtcatcac cgtccgcaac cgcgccggcc
     1141 gctcaccgag ccgccacaac tgaattccca tccccgccgc cggtcgtccc gccgtgagat
     1201 cgagaacatg ggtgctgagt ttcgcaggca ttttatgaaa gcaccaagct ccaagcacca
     1261 agctccagag aagcaccaac ccgcaatctc caaatttgaa tcgtggtcat tgattcttct
     1321 cgggagcttg gaacttggtg cttggtgctt gattgaccgc atcctgcagt cgcagccacg
     1381 cgatcttctc gatctcacgg agcgccgccg cgtgctctgc ttccggcgtg ttcttcaacc
     1441 gctcccgcat cgcgcgcaaa atcgcatcgc gtgcattcaa ccgcgcacaa atcacaaacg
     1501 gaaaaccaaa ccgctcgcga tactccgcgt tcagccgctg aaaattcgcc agctcctcat
     1561 ccgacaactg atccagcccc gcactcgcct gttcgcgcgc cgactccgcc gtcagcttct
     1621 tctcttgcgc catccgcccc gccaaatccg gatgcgcccg aatcagctcc agctgcttct
     1681 cctttggcgc cgccctcatc gtctcacaaa gctccgcgtg caatgctgcg gtatcccgaa
     1741 acggccgctt cgcccacgcc tcctccgcca cccaaggcga atgctcgaac aggtgcccga
     1801 gcgcagcggt gaacgcagcg cgatcgagtt ggttgagctg tgcgagcgtc atcatcgcgc
     1861 cgagttcaaa gctccaagca ccaagctcca agctccagag aaaccccaac cctcaactcc
     1921 caatcttgaa ttttgttcat tggttcttct ctggagcttg gatcttggtc cttggagctt
     1981 ccttgagcta cgccagtagc tcgtaggccg gcagcgacaa aaactcgtcg aaggtctcgc
     2041 tcgtcaccat ccgcatgaac agcgccgtcg cctccttgaa atgtcccgca tcgtaaaccc
     2101 cggcgccata ctcgccgcga atcttcacca gctcatccgc gagcagcgtc tcatagaact
     2161 ccaccgtgat cgcgcgtccg tcgtccagct tcgcgccaaa cttcagccat tgccacaact
     2221 gcgaacgcga gatctccgac gtcgccagat cctccatcaa ataatgaatc ggctccgccc
     2281 ccgacccgcc
//
LOCUS       DASPLT010001074         1331 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_177437, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001074 DASPLT010000000
VERSION     DASPLT010001074.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1331)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1331)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1331
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_177437"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..208
                     /locus_tag="VEA63_11090"
     CDS             <1..208
                     /locus_tag="VEA63_11090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HYD84594.1"
                     /translation="RHPDEEIIIVKEGRVEATINGQSKTAGPGSIFFFASNDEHGLRN
                     VGDTQATYYVLRVGTEKTPALAAK"
     gene            305..1093
                     /locus_tag="VEA63_11095"
     CDS             305..1093
                     /locus_tag="VEA63_11095"
                     /inference="COORDINATES: protein motif:HMM:NF018178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HYD84595.1"
                     /translation="MNHSLPRRSSVFAFAALTVAFASPALAAIGLDAKPAAGAEVIID
                     GTRQTLDEKWTYWEGPRFSSSLPIKWKIVEDPVDGGTVVQTFDPAAAGGKYGAADIIT
                     KKVYRDFRLHIEFLVTAEGGNSGVYLQNRYEIQICDGDKTKHGMGAVINETESPYHCY
                     RGLGQWNAYDIVFRAARFKDGQRVEPAMVTMYFNGEKVHHNQPIQQVWGGPNSGLDGG
                     NDKGKGITDVPGPLKLQAEGHDVRYRNAWIKELDLAQPDTDFSE"
BASE COUNT          284 a          480 c          351 g          216 t
ORIGIN      
        1 ccgccacccc gacgaagaaa tcatcatcgt gaaagaaggt cgcgtcgaag ccaccatcaa
       61 cggtcaaagc aagaccgccg gcccgggctc catcttcttc tttgcgtcga acgacgagca
      121 cggacttcgc aacgtcggcg atacgcaggc cacctattac gtcctccgcg tcggcaccga
      181 aaaaacgccc gcgctcgccg cgaaataaag cgtagcggca ggcctccgtg cctgccagct
      241 tctgcagcga cgttaagccg aaagcacgcc cgaaggcgtg gcaccacatc cctccttcta
      301 ccccatgaac cattccctac cccgccgctc ctccgtgttt gcattcgccg ccttgaccgt
      361 cgcgttcgcc tcgcccgcgc tcgccgcgat cggcctggac gccaaacccg ccgccggcgc
      421 cgaggtcatc atcgacggca cgcgtcaaac cctcgatgaa aagtggacct attgggaagg
      481 tccccgcttc agttcttcgc tcccgatcaa gtggaagatt gtcgaggatc cggtcgacgg
      541 cggcaccgtt gtgcaaactt tcgatcccgc ggctgccggc ggcaaatatg gcgccgccga
      601 catcatcacg aaaaaggtct accgcgattt ccgccttcac atcgaattcc tggtgaccgc
      661 cgagggcggc aacagcggcg tgtatctaca aaatcgttac gagatccaga tctgcgacgg
      721 cgacaaaacc aaacacggca tgggcgccgt catcaacgaa accgaatcgc cctatcactg
      781 ctaccgcggc cttggccaat ggaacgccta cgacatcgtg ttccgcgccg ctcgcttcaa
      841 ggatggccag cgcgtcgagc ccgcgatggt cacgatgtat ttcaacggag aaaaagtgca
      901 ccacaaccag cccatccaac aggtctgggg tggcccgaat tccggtctcg atggcggcaa
      961 cgacaaaggc aaaggcatca ccgacgtccc ggggccgctc aagctgcaag ccgaaggtca
     1021 cgacgttcgc taccgcaacg cctggatcaa ggagctcgat ctcgcgcaac ccgacaccga
     1081 cttttccgag tagggcgggc ttcagcccgc tctcaaaacc gcccgtcgca ccacaacgtt
     1141 gcctaccgcg atcgcacagc caacgctgac gccgccatga gctcacccca ctccgaaaat
     1201 cccgcggcgg aggccgcccg cgaactcgcc cgcgccgccg cgaagctgct cgccaccgag
     1261 ggcgaagccg cattctcccg tatcgagtca ccgccgtttc gctccggcga cgattacgtc
     1321 ttcgtcttcg a
//
LOCUS       DASPLT010001075         1899 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_217118, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001075 DASPLT010000000
VERSION     DASPLT010001075.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1899)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1899)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1899
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_217118"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(101..>1899)
                     /locus_tag="VEA63_11100"
     CDS             complement(101..>1899)
                     /locus_tag="VEA63_11100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012372932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA gyrase/topoisomerase IV subunit A"
                     /protein_id="HYD84596.1"
                     /translation="GRFHKVANVVGRTMSLHPHGDASIGAALVAIGQRAFLIEPQGNY
                     GNLLTGDEAAAPRYIEARLTNFAKDVLFNPKTTTWQLSYDGRAREPVTLPAKFPIVLL
                     EGAEGIAVGLSTKILPHNFNDLCRAAINHLQGKTFRIYPDFPTGGIADFSEYNDGERG
                     GKVKVRAKIEVRSKYLLAITELPFGATTESLIESILAANAKGKIKIKHVDDNTADSVE
                     ILVHLPQGSEPEKIIQQLYVFTGCQANLSPAACVIENDRPHFLGVREILKRSADKTKE
                     LLKRELEIKLEELEQQWHWDSLERIFIEERIYRRIEKSKTWESVLEEIREGLKPFLKQ
                     LRRPVTDDDITRLTEIRIKRIAAYNRFQADEAIAKIETGIKETKANLKNLTAHAVAWF
                     EMLQEKYGKGLKRKTSYDEIEQINAAEVVSANQRLYVNREDGFIGLNWRQHEFVQECT
                     ILDSALCIMRDGSLKVAKVGEKVFMGRDIIHVGVFPKDGDPNFYTMVYQDKESGKAFA
                     KRFQIGGLSRDKLYPLVKSEGSKVIYLDVSKREKDMPKKLQIFIDGRSGARVRELEFD
                     LKTVPVSSRSAKGITVTKWPVKDVKRVDLALQ"
BASE COUNT          329 a          660 c          483 g          427 t
ORIGIN      
        1 cgtctcccgc tgctcacgcg gcgagccacc gcgatcgtgg ctctgcgcgt gagcgcagga
       61 atcggtagct ctgcgcgtgc gcgcagggag atcatgcgct ctactgcaac gccagatcca
      121 cccgcttcac atccttcacc ggccacttcg tcaccgtaat cccctttgcg ctgcgcgagc
      181 tcaccggcac cgttttcaga tcgaactcca gctcgcgcac gcgcgcgccg ctgcgtccat
      241 cgatgaaaat ctgcagcttc ttcggcatgt ccttctcgcg cttgctcaca tcgagataga
      301 tcaccttcga accctcggat ttcaccagtg gatagagctt gtcgcgcgag agcccgccga
      361 tctggaaccg cttcgcaaac gccttcccgc tttccttgtc ctgataaacc atcgtgtaga
      421 agttcgggtc gccgtccttc ggaaacacgc cgacatggat gatgtcgcgc cccatgaaaa
      481 ccttctcccc gaccttcgcg actttcagcg aaccgtcgcg catgatgcag agcgcgctgt
      541 cgagaatcgt gcactcttgc acgaactcgt gctgccgcca gttcagcccg atgaagccgt
      601 cttcgcgatt cacatacaaa cgctggttgg ccgacacgac ttccgccgcg ttgatctgct
      661 cgatctcgtc gtaactcgtc ttccgcttca gtcccttgcc gtatttttcc tgcagcatct
      721 cgaaccacgc gaccgcatgc gccgtcaggt tcttcagatt cgccttcgtt tccttgatgc
      781 ccgtctcgat cttggcgatc gcttcgtccg cctggaaacg attgtaggcc gcgattcgtt
      841 tgatccggat ttccgtcagc cgcgtgatat cgtcgtccgt caccggccgg cgcagctgtt
      901 tcaaaaacgg cttcaaccct tcgcggatct cctcgagcac gctttcccac gtcttggatt
      961 tttcaatccg acggtaaatg cgctcctcga tgaaaatccg ctcgagcgaa tcccagtgcc
     1021 actgctgctc cagctcctcg agcttgatct cgagttcgcg cttcagcagc tccttcgtct
     1081 tgtccgcact gcgcttcagg atctcgcgca cgccgaggaa atgcggccgg tcgttctcga
     1141 tcacgcacgc cgccggcgac agattcgcct ggcagccggt gaacacataa agctgctgga
     1201 tgatcttctc cggctcgctg ccctgcggca ggtgcacgag aatctccacc gagtccgcgg
     1261 tgttgtcgtc gacgtgcttg atcttgatct tgcccttcgc attcgccgcg agaatcgact
     1321 cgatcaacga ctccgtcgtc gcgccaaacg gcagctccgt gatcgccagc aggtatttgc
     1381 tccgcacttc gatcttcgcg cgcaccttca ccttcccgcc gcgctcgccg tcgttgtatt
     1441 ccgaaaaatc cgcgatgccg cccgtcggga agtccggata aatccgaaac gtttttccct
     1501 gcaggtgatt gatcgccgcc cgacacaggt cgttgaaatt atgcggcagg attttcgtcg
     1561 agagtccgac cgcgatgcct tccgcgcctt cgagcaacac gatcggaaac ttcgccggca
     1621 acgtcaccgg ctctcgcgcg cgcccgtcgt agctcaactg ccacgtcgtc gtcttcgggt
     1681 tgaacaacac gtcctttgcg aaattcgtca gccgcgcttc gatgtaacgc ggcgccgccg
     1741 cttcgtcgcc cgtcagcaaa tttccatagt tgccctgcgg ctcgatcaaa aacgcccgct
     1801 gcccgatcgc gaccagcgcc gcgccaatcg acgcatcgcc gtgcggatgc agcgacatcg
     1861 tgcgacccac gacattcgcg accttgtgaa accgcccgt
//
LOCUS       DASPLT010001076         2073 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_93861, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001076 DASPLT010000000
VERSION     DASPLT010001076.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 2073)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2073)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2073
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_93861"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..973
                     /locus_tag="VEA63_11105"
     CDS             <1..973
                     /locus_tag="VEA63_11105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012372989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HYD84597.1"
                     /translation="CSRVAGYFRGDNLVIRHCLVSDCGTEGIYVINSADVLLEKNIVT
                     RTNSAEKISGYFASSVKIFNQSYRTVCRDNLIIDNPHASGIWYDVGNVDGVFVNNWIE
                     RTNDGFFFEISKGAICAGNVFVNCNKGIRILNSSKVQVYQNTFFNSVASFERSERSAV
                     GDHFGWHPSAGPDVHERHDHVFVNNILAADESFKGPLVQFQQTAAVKDRLKDPQVKEI
                     DANIYVRRAGAVPQPLIAWSPVQSEKGSVEIAALEELRKVNKKFEANGEAFPDYRGLL
                     FRGVELGNFELLSAFPAAKRAAALPAHIREVLGWKEGTFPGAYAPIR"
     gene            1126..>2073
                     /locus_tag="VEA63_11110"
     CDS             1126..>2073
                     /locus_tag="VEA63_11110"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HYD84598.1"
                     /translation="MFVAAVVARGQTPTVVQALPTRTLVAGGASASIHLRDYFGLPTV
                     TGPVVQFDTSKGKFNVELFSADTPKTAANFVSYTRRGAYVNSFVHRTVNNFVIQGGGF
                     VLNGNSVDPIVTDPPIENEAKFSNTRGTIAMAKTPVGPHTATSQWFINLKNNATPLDT
                     ENGGYTVFGRVLGTGMTVVDAIAAVPTYNASATLGAAFGELPLLNNELIAANFVMSSI
                     RSAPVFPEVASATSALAFSATSSNPAVAAVEVVASTLMVKPIAAGSTIVTARVVDPNG
                     NAQQTSFTATVVGDPSFITEPVSQAVAQGGGGTLSAAATG"
BASE COUNT          395 a          553 c          692 g          433 t
ORIGIN      
        1 ttgctcgcgc gtggcgggtt atttccgcgg cgacaatctg gtgatccggc actgcctggt
       61 ttcggactgc ggcacggaag gcatttacgt gatcaactcg gcggatgtgt tgctcgagaa
      121 gaacatcgtg acgcgcacga acagcgcgga aaaaatctcg ggttacttcg cgtcgtcggt
      181 gaagattttc aaccagtcgt atcgcacggt gtgtcgcgac aacctcatca tcgacaaccc
      241 gcatgcgagc ggcatctggt atgacgtcgg caacgtcgac ggcgtgttcg tgaacaactg
      301 gatcgagcgg acgaacgacg gtttcttctt tgagatctcg aagggcgcga tctgcgcggg
      361 gaatgttttc gtgaactgca acaagggcat tcggattttg aacagctcga aggtgcaggt
      421 gtatcagaac acgtttttca acagcgtggc gtcgtttgag cgatcggagc gcagcgcggt
      481 gggcgatcat ttcggctggc atccgagtgc gggtccggac gtgcatgagc ggcatgatca
      541 tgtgttcgtg aacaacattc tggcagcaga cgagtcgttc aaggggccgc tggtgcagtt
      601 ccagcagaca gcggcggtga aggaccggtt gaaggatccg caggtgaagg agatcgacgc
      661 gaacatctat gtgcggcggg cgggtgcggt gccgcagccg ttgatcgcgt ggagtccggt
      721 gcagagcgag aaaggctcgg tcgagatcgc ggcgctcgag gaactgcgga aggtgaacaa
      781 gaaattcgag gcgaacggcg aggcctttcc ggattaccgc gggctgctgt tccgtggcgt
      841 ggagctgggc aacttcgaac tgttgtcggc gttcccggcg gcgaagcgcg cggcggcgtt
      901 gccggcgcat attcgcgaag ttcttggctg gaaagaagga acgtttcccg gcgcgtatgc
      961 gccgattcgc tgagcgcacg gagcggccgc ggcggatacg ttcgccgcgg cggttcggag
     1021 tcacgatttt ttttcgcgcc gacgaataaa gggtttgtcg gcgccgcggt gacgcttgag
     1081 tttggcgcga tgatttcgaa gcgccttctc tgttgtttcc tttctgtgtt cgttgcggcg
     1141 gtcgtggcgc gtggtcaaac gccgacggtg gtccaagcgt tgcccacccg gacattggtt
     1201 gcgggcggcg cgtccgcctc gattcacctg cgcgattatt tcggcctgcc gaccgttacc
     1261 ggaccggtcg tgcaattcga cacctcgaag gggaaattca acgttgagct gttttccgct
     1321 gatacgccga aaacagcggc gaacttcgtg agctacacac gtcgcggcgc ctatgtgaac
     1381 agcttcgttc accgcaccgt gaacaatttt gtaattcaag gcggcggctt cgtgcttaac
     1441 ggcaacagtg tcgatccaat tgtcaccgat ccgccgatcg aaaatgaggc gaaattttcg
     1501 aatacgcgtg gcacaatcgc gatggcgaaa acgcccgtcg gcccgcatac cgcaacctcg
     1561 cagtggttca tcaatctcaa gaacaatgcg acgcctcttg atacggaaaa cggcggctat
     1621 acggttttcg gacgcgtgct tggcacgggc atgacggtgg tggatgcgat cgcggctgtg
     1681 ccgacttaca acgcgagcgc aacgctgggc gcggctttcg gcgagcttcc gttgctgaac
     1741 aatgagctga tcgcggcgaa ttttgttatg agcagcattc ggtcggcgcc ggtgtttcca
     1801 gaagtggcga gcgccacgtc ggccctggcg tttagcgcga caagcagcaa tccggccgtt
     1861 gctgcagtgg aagtcgtcgc ctcgacgttg atggttaaac cgatcgcggc tggctccact
     1921 atcgtgacgg ctcgcgtggt cgatccgaac ggaaatgcgc agcaaaccag cttcacggcg
     1981 acggtcgtcg gcgatccctc gtttatcacg gaaccggtga gccaggcggt agcgcagggc
     2041 ggcggcggga cgttgtcagc ggcggcgacc ggc
//
LOCUS       DASPLT010001077         3483 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_36664, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001077 DASPLT010000000
VERSION     DASPLT010001077.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 3483)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3483)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3483
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_36664"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..616
                     /locus_tag="VEA63_11115"
     CDS             <1..616
                     /locus_tag="VEA63_11115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD84599.1"
                     /translation="RSLVPLVLCLALPCGPLGCSSMGPGTAAVATVSAVAVGGTLSPL
                     NSAYDAVTNKEEKTRARFAALHARLDPICEQKIAAILARDPIADARMLHAAGVVAPLS
                     SVPGNIVYPGLWGNRYDVSKLVQNDTAARDSALYQELATLMNPYAEDLTPSPDEPRHY
                     HNEVFRRFLEVAGDYKATFNREMYRLETGYSEPPEPARRRLRQT"
     gene            669..1061
                     /locus_tag="VEA63_11120"
     CDS             669..1061
                     /locus_tag="VEA63_11120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012377099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 family protein"
                     /protein_id="HYD84600.1"
                     /translation="MSLHVVRLGTPRLPDEGVRIGTVRRPPRGVPKSQFASHNWYDVW
                     FPNLAPSIETMRLGLAAKAPAQWSTFARRYKAEMAKPAAQHDLALLATLSHTTNFSVG
                     CYCEDESRCHRSLLRQLLADCGAKIEPA"
     gene            1273..2373
                     /locus_tag="VEA63_11125"
     CDS             1273..2373
                     /locus_tag="VEA63_11125"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HYD84601.1"
                     /translation="MQLPLFSVLSGLLLLLTLLPLSKRPEWWIRGLDFPRFQLAVLGL
                     ILLAAGAFTLDWHHPGTWAAGLVVLGCLVFHAWWIFPYTPFHRKEVKSAPRTPPDRRL
                     RLMAANVLMTNRRAVDLRALVRRHRPDVLITLETNRWWEQQLAPLERDYPHTLKCPLE
                     NLYGMHLYSRLPLEDARVQFLVEDNVPSIHTLVILRCGQPVRLHCLHPAPPSPTENPT
                     SRERDAELVMVGKAVVGASLPVVVTGDLNDVAWSDTTRLFRKISGLLDPRIGRGRFNT
                     YHARHAFLRWPVDHFFHSAHFTCFELKRLPAFGSDHFPVLVDLAFGEPHQTSELAAGS
                     EDEHEARDKMREQAATETEVHRPGESSASGRR"
     gene            2604..3050
                     /locus_tag="VEA63_11130"
     CDS             2604..3050
                     /locus_tag="VEA63_11130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5069 domain-containing protein"
                     /protein_id="HYD84602.1"
                     /translation="MKIPGLRSAHDEVGGIVYFGRMLDKIRLKAAGQLPADYNTGTRS
                     WFDFDARCCRFLRVRYAALRNRTLEGGSDLQILRGCFKEGRKPSSEDIEIWNTFMRKR
                     GWRDPSSEGLDADKRRAGLGKRDDILTWFDLFDAEEERLPRAKYSA"
     gene            complement(3078..>3483)
                     /locus_tag="VEA63_11135"
     CDS             complement(3078..>3483)
                     /locus_tag="VEA63_11135"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HYD84603.1"
                     /translation="KELSIALGAKRNTPSGLGGHPFDLELSKLRPLGCGSPFHSHAAE
                     WELFMILSGTATVRADAATHTLVAGDVVLHPPGEAHQITNASATEELVFYLVADNAPV
                     EYWHYPDTNKWGLRSPRKFFRMEEREYWADEE"
BASE COUNT          623 a         1354 c          882 g          624 t
ORIGIN      
        1 ccgctcgctc gtgccgctcg tcctctgcct ggcattacca tgcggcccgc ttggctgctc
       61 ttcgatgggc cctggcaccg ccgccgtcgc caccgtcagt gctgtcgccg tcggcggcac
      121 gctgtcgccc ctgaacagcg cttacgatgc ggtcaccaac aaagaggaaa aaacgcgcgc
      181 gcgtttcgcc gcactccacg cccggctcga tccgatctgc gaacaaaaaa tcgccgccat
      241 cctcgcgcgc gatcccatcg ccgacgctcg catgctgcac gccgccggcg tcgtcgcgcc
      301 gctctcgtcg gtgcccggca acatcgttta ccccggcctc tggggcaatc gctacgacgt
      361 ttccaaactc gtccaaaacg acaccgccgc acgcgactcc gcgctctatc aagaactcgc
      421 cacgctgatg aacccgtatg ccgaggatct cacgccgagc ccggacgaac cgcgtcatta
      481 ccacaacgaa gtcttccgcc gcttcctcga agtcgccggc gactacaaag cgacattcaa
      541 ccgcgagatg tatcgcctcg aaaccggcta cagcgaaccg cccgaaccgg cacgccgccg
      601 acttcggcaa acctgatgcc acgccatcgt cgtttcagcg aaaaaacaga cctcagtccc
      661 tcgcccccat gagcctccat gttgtccgcc tcggcacgcc tcgtcttccc gatgaaggcg
      721 ttcgcatcgg cacggttcgc cgcccgccgc gcggtgtgcc gaaatcgcaa ttcgcctcgc
      781 acaactggta tgacgtgtgg ttccccaacc tcgcgccgag catcgagacc atgaggctcg
      841 gcctcgccgc caaggcgccc gcgcagtggt cgaccttcgc gcgccgctac aaagccgaga
      901 tggcgaagcc cgccgcccaa cacgatctcg cgctcctcgc cacactttct cacacgacca
      961 atttttccgt cggctgctat tgcgaagacg aatcccgctg ccaccgctca ctgctgcgcc
     1021 agttgctcgc cgactgcgga gcaaaaatcg agcccgcgta atcccggcaa cctccgtcgc
     1081 cgacttcgtc acgaacacaa aaggcggagc ccgacgtcct cgtcgggctt gatcaccttt
     1141 cgctcgcaag cagccgcact ccgcgctcgt cgcgcgccgg tttgctccac acccgaacca
     1201 acgcgccgca ttcgggtcgt taacgcgcag ccccacgagc accgcttttt ccctcacctc
     1261 gctgcgcccc ccatgcagct ccctctcttc agcgtcctct ccggtcttct cctgctgctc
     1321 accctcctgc cgctatcgaa acgcccggaa tggtggattc gcggcctcga cttcccccgc
     1381 tttcaactcg ccgtgctcgg tctcatcctg ctcgcggccg gcgcatttac actcgactgg
     1441 catcatcccg gcacatgggc cgccggcttg gtcgtcctcg gctgcttggt tttccacgcg
     1501 tggtggattt ttccctacac gcccttccac cgcaaggagg tgaaatccgc cccgcgcacg
     1561 ccgccggacc gccgcctgcg cctcatggcc gccaacgtcc tcatgaccaa tcgccgcgcc
     1621 gtcgacctcc gcgcgctcgt ccgccgtcac cgccccgatg tcctcatcac cctcgaaacc
     1681 aatcgctggt gggaacaaca actcgcgccg ctcgagcgcg attatccaca cacgctcaaa
     1741 tgcccgctcg aaaatctcta cggcatgcat ctctactcgc gcctgcccct cgaagacgcg
     1801 cgcgtgcagt tcctcgtcga ggacaacgtg ccttcaatcc acacgctcgt catcctgcgg
     1861 tgcggccaac cggtgcggct tcactgcctg catccggcgc ctccgagtcc gaccgaaaac
     1921 cccacctcac gcgaacgcga cgccgagctc gtgatggtcg gcaaggccgt cgtcggcgcc
     1981 tcgctgccgg tcgtcgtcac cggcgatctg aatgatgtcg cgtggtccga cacgacgcgt
     2041 ctcttccgaa aaatcagcgg cctcctcgac ccgcgcatcg gccgcggtcg tttcaacacc
     2101 taccacgccc gacacgcgtt cctccgctgg cccgtcgatc actttttcca cagtgcgcac
     2161 ttcacctgct tcgaactgaa gcgcctcccc gccttcggct ccgatcactt tcccgtgctc
     2221 gtcgacctcg ccttcggcga accgcaccaa acctccgagc tcgccgccgg ctccgaggac
     2281 gagcacgaag cacgcgacaa gatgcgcgaa caagccgcca ccgaaaccga agttcaccgc
     2341 cccggcgaat cctccgcttc tggtcggagg taggagcctg cttgcaggcg atttcgatct
     2401 gcgaggagaa acatcgcctg caagcaggct cctacaccgc caatgaaacc cctgcagatc
     2461 ccgacgaacc gtccgcgctt cgccgatcag gcgaagcacg caatgcttcc cagccggact
     2521 acctccgaaa aatcagcgca gatcagcggt tcacaccgtt tcttccctga atctcccctg
     2581 aacctcatcc gacagttctc tccatgaaaa tccccggact ccgcagcgcg catgacgaag
     2641 tcggcggcat cgtctacttc ggtcgcatgc tcgacaagat tcgcctcaag gccgccggtc
     2701 agctccccgc cgactacaac accggcactc gctcctggtt cgatttcgac gcgcgctgct
     2761 gccgcttctt gcgcgtccgc tacgccgccc tgcgcaaccg caccctggag ggcggctccg
     2821 atctccaaat cctgcgcggg tgcttcaagg aaggtcgcaa acccagttcc gaagacatcg
     2881 agatctggaa cacctttatg cgcaaacgcg gctggcgcga tccgtcgagc gagggcctcg
     2941 atgccgacaa acgccgcgcc ggacttggca aacgcgacga catcctgact tggttcgact
     3001 tgttcgacgc cgaggaggaa cgccttccgc gtgcgaaata ttccgcgtaa cccccaaaag
     3061 cgtccgcttc tcagcgctca ctcttcgtcc gcccaatatt ccctctcctc catgcggaaa
     3121 aattttcgcg gcgaacgcag cccccacttg ttcgtgtcgg gatagtgcca gtattcgacc
     3181 ggcgcgttgt ccgccacgag atagaacacc agctcctccg tcgccgacgc gttcgtaatc
     3241 tgatgcgcct cgcccggcgg atgcagcacc acatcgcccg ccaccaacgt gtgcgtcgcc
     3301 gcatccgccc gcaccgtcgc cgtcccgctc aggatcatga acagttccca ctccgccgca
     3361 tgactgtgaa acggcgaccc gcatccgagc ggccgcagct tgctcaactc caagtcgaac
     3421 ggatgcccgc ccaacccgct cggcgtgttg cgtttcgcac cgagcgcgat cgagagctct
     3481 ttg
//
LOCUS       DASPLT010001078         2104 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_52465, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001078 DASPLT010000000
VERSION     DASPLT010001078.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 2104)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2104)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2104
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_52465"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..488)
                     /locus_tag="VEA63_11140"
     CDS             complement(<1..488)
                     /locus_tag="VEA63_11140"
                     /inference="COORDINATES: protein motif:HMM:NF021778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-propeller fold lactonase family protein"
                     /protein_id="HYD84604.1"
                     /translation="MMFRFLLSLAVFGGVVNAQAREHVALLGTYTGESRGIYSVRLDS
                     ETGAVSTPELVAELPNPEFLALHPSGRFVYALTQVAGADGKNTGAVAAFEVDAETGRL
                     TPLNVESTGRGSLCHLAVDGSGRMAIVTSYGGGYVASFPIGTDGRVGARASLVSHEGP
                     LGP"
     gene            complement(647..976)
                     /locus_tag="VEA63_11145"
     CDS             complement(647..976)
                     /locus_tag="VEA63_11145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007362568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HYD84605.1"
                     /translation="MTLTLPPLLAAMIAAHNAHDSTAFAACFTDDAIVRDEGRRHYGK
                     AAVKAWFEDVSGKYGAVAEVTDLTLVDGEPVLSVRVSGDFEGSPIELRYFTALEDGKI
                     VALKIAP"
     gene            1132..>2104
                     /locus_tag="VEA63_11150"
     CDS             1132..>2104
                     /locus_tag="VEA63_11150"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HYD84606.1"
                     /translation="MSTSRHPRALSRFIHAAFATLLAITCGPRAVAQLAPAAAPESIV
                     ELERFIITESALARSGDLLPTSRPSDSVFGSSAPVLDLPRAITVVTPALIEKFGLRDF
                     ADLGRVAAGAERPNYYGVPGAPVLRGDFAGTFFNGMQRAWQRNEMPMSFGSLEGMDVV
                     KGAAPANFGPTQGGGYVNFLPKSPYYDRRRGSLRIAAGSYGYLNSAFDIGGPFLFGDR
                     PAALRVSLTAQKADAYARNVANDYLSLYAALKLQLADGLSLFTGAEYYTFRANENAGW
                     NRVTQDLIDHGDYIVGEVRDTTSAAAGGQVLPSDVPFIPVFGAALPGS"
BASE COUNT          379 a          789 c          563 g          373 t
ORIGIN      
        1 gggccgagcg gcccttcgtg cgaaaccaaa ctcgcgcgcg ctcccacccg gccatccgtt
       61 ccaatcggaa acgaagcgac ataaccgccg ccataactcg tcacgattgc catgcggccg
      121 ctcccgtcga cggcgaggtg acaaagcgag ccgcggcccg tcgattccac attcaacggc
      181 gtcaaccgtc ctgtttccgc atccacttcg aaggccgcaa ccgcaccggt gtttttgcca
      241 tcggcgccgg caacctgggt cagcgcgtaa acgaagcggc cgctcggatg cagcgcaagg
      301 aactcgggat tcggcaactc ggccacgagt tccggcgtcg acaccgcgcc cgtttcggaa
      361 tcgaggcgca cggaataaat cccgcggctt tcgccggtgt aggtccccaa cagcgcgaca
      421 tgctcgcgtg cctgcgcgtt caccaccccg ccgaacaccg ccaacgaaag aagaaagcga
      481 aacatcatgg cgggagcctg agcatgcacc gcacggcgcg cacgctttta ccgtattcca
      541 aaccaaacca cacttcccct gtagggcggg ttctcccgac cccgccgctc cgctcactcg
      601 ccctctcccg ccgcactacc ctctcgcacg tccaatcccg ccgaactcac ggcgcaatct
      661 tcagcgcaac aatctttcca tcctccaacg ccgtgaagta acgaagctcg atcgggcttc
      721 cctcgaaatc gccggaaact cgcaccgaca aaaccggctc cccatccacc agcgtgagat
      781 ccgtcacctc agccaccgcg ccatatttcc cgctcacgtc ttcgaaccat gcctttacgg
      841 ccgccttgcc gtaatggcgc cggccttcgt cgcggacaat cgcgtcgtcc gtgaaacacg
      901 cggcaaatgc cgtgctgtcg tgagcgttgt gcgccgcgat catcgcagcg agcagcggag
      961 gaagggtaag agtcatgctc gcaacgtaac acggaccact gacaacccta tgtcatcaga
     1021 gattcgcccc gtgtgcggct gacgttgaag aaatttcgat tgcgaaactc ctcgcgcgtt
     1081 cttgcctccg ctcccgtcgc acctccgcgc gcgacatccc gcttccacac catgtcgacc
     1141 tcgcgccacc cacgcgccct gtctcgtttc attcacgctg cttttgccac gctgctcgcg
     1201 atcacatgcg gtccgcgcgc cgtcgcccag ctcgcacccg ccgccgcccc ggagtccatt
     1261 gtggaactgg agaggttcat catcaccgaa tcggcgctcg cccgttccgg cgatctgctg
     1321 ccgacgtctc gcccgagcga ctcggtcttc ggttccagtg cgccggtcct cgacttgccg
     1381 cgcgcaatca ccgtcgtcac ccccgcgctc atcgagaaat tcgggctccg tgacttcgcc
     1441 gatctcggcc gcgtcgccgc cggcgccgag cggcccaact actacggcgt ccccggcgcg
     1501 cccgtgcttc gcggcgattt cgccggcacg tttttcaacg gcatgcaacg cgcgtggcag
     1561 cgcaacgaaa tgccgatgtc cttcggttcg ctcgaaggca tggacgtggt gaagggcgcc
     1621 gcgccggcga acttcggccc gacccaaggc ggcggttacg taaatttcct gcccaagtcg
     1681 ccttactatg atcggcgccg cggctcgctg cgcatcgccg ccggcagcta cggttatctc
     1741 aacagcgcgt tcgatatcgg cggtccattt ctcttcggtg accgtcccgc ggcccttcgc
     1801 gtctcgctga ccgcgcaaaa ggccgacgcc tacgcacgca atgtcgcgaa cgactatctt
     1861 tcgctctacg ccgcgttgaa acttcaactc gccgacggtc tctcgctctt caccggcgcg
     1921 gaatactaca cgtttcgcgc caacgaaaac gccggctgga accgcgtcac ccaagatctg
     1981 atcgaccacg gcgactacat cgtcggggaa gtgcgcgaca ccaccagcgc cgccgccggc
     2041 ggccaggttc tcccgagcga cgtgccgttc atccctgtat tcggcgcagc cctaccgggc
     2101 agct
//
LOCUS       DASPLT010001079         2045 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_278560, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001079 DASPLT010000000
VERSION     DASPLT010001079.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 2045)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2045)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2045
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_278560"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2008)
                     /locus_tag="VEA63_11155"
     CDS             complement(<1..2008)
                     /locus_tag="VEA63_11155"
                     /inference="COORDINATES: protein motif:HMM:NF019219.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1549 domain-containing protein"
                     /protein_id="HYD84607.1"
                     /translation="MPPSKPPVPSVGEWARNPIDAFVLRRLQAEGLRPSSPASPDAWL
                     RRVSLDLTGLPPSPVDADAFARDVKARGEKAYSAAVDRLLASPAYGERMALDWLDVAR
                     YADTHGFNNDAERSMWRWRDWVIDSFNANLPYSSFITEQLAGDLLPNSTLEQRIATGF
                     GRNHVINSEGGIIEEEYRVEYVADRVRTLGMAWLGLTFECARCHDHKYDPISQRDHYR
                     LYAFFNNVPELGEDGRVANAVPMIPAPTREQQAELKRLEGLIASARKKLEAREKAWAW
                     RAQHNFPKQTAAEPADRALYIGCESAEQAAVEIAPGPAGNACTVAEAAGAWHATEVRI
                     ARRAPLTFSAWIRPAQADIDAPILSTANYSDPPEATSHGKGMEFRLNGGELEFRLNDR
                     FPAYAVRVRSQGANLHAGQWRHVAVVYGGAPEEAMRVHASSVRMFVDGREVATSAVND
                     GAMLSSVKNDKPAPAKFRVGWDNSPKKGRFTGQLDELSLWYRALQPDQIAAAFERHAL
                     PWAASRDSRSALEASWLRHSQLQQTDRKYGEEYRRYNDAHTALLALRRSLPTVMVMSD
                     AAPRKTRLLKRGAYNAPGEEVAPGVPEELLGAWPADAPKNRLGLAKWLTQPNHPLTSR
                     VVVNRFWQQLFGQGLVKTSDNFGMQGDWPSHPDLLDWLARDFVDS"
BASE COUNT          360 a          647 c          672 g          366 t
ORIGIN      
        1 cggagtccac gaagtcgcgc gccagccagt cgagcaggtc ggggtgcgac ggccagtcgc
       61 cctgcatgcc gaagttatcg ctcgtcttta ccaggccttg gccgaagagc tgctgccaga
      121 atcgattgac gacgacacgg ctcgtcaacg gatgattcgg ctgagtgagc cacttcgcga
      181 gaccgagccg gttcttcgga gcatccgcgg gccacgcgcc gagcagttcc tcgggaactc
      241 cgggcgcgac ttcctcgccg ggtgcgttgt acgcgccgcg cttcagcaga cgggtcttcc
      301 gcggagcggc gtcggacatc accataacag tgggaagact gcgtcgcagc gcgaggagtg
      361 ctgtgtgcgc gtcgttgtag cgacggtact cttcgccata cttccggtca gtctgctgga
      421 gttgtgagtg ccggagccaa ctggcttcaa gtgcagagcg actatctctg gaggcggccc
      481 acgggagggc gtgccgctcg aaggccgcgg caatctggtc cggctggaga gcgcgatacc
      541 aaagggacag ctcgtcaagc tggccggtga agcgaccctt cttcggactg ttgtcccagc
      601 caacgcggaa cttcgccggg gcgggcttgt cgtttttcac cgaactgagc atcgcgccgt
      661 cattcacggc gctcgtggca acctcacgtc cgtcgacgaa catgcggacg gaggaggcgt
      721 ggacgcgcat ggcctcttcg ggcgcgccgc cgtagacgac cgctacatgc cgccactgcc
      781 cggcgtggag gttcgcgcct tgcgaccgga cgcgaacggc gtaggcggga aaccgatcat
      841 tgagacggaa ttcgagctca ccgccattca gccgaaattc catgcccttg ccatgcgagg
      901 tggcctcggg gggatcgctg tagtttgcgg tggaaaggat cggcgcgtcg atgtctgcct
      961 gggcggggcg aatccacgca ctgaaggtga gcggcgcccg gcgcgcgatc cggacctccg
     1021 tcgcgtgcca cgcgcctgcc gcctccgcga cggtgcatgc atttcccgca ggccccggag
     1081 cgatctcgac agctgcctgt tcggccgact cgcaaccgat atacaaagcg cgatccgcgg
     1141 gttcagcagc cgtctgcttc gggaagttgt gttgagcgcg ccacgcccac gccttctcac
     1201 gcgcctccag ctttttccgt gcggaagcaa taagaccctc aagcctcttc agctcggcct
     1261 gctgctcgcg cgtgggggcc gggatcatcg gaacggcgtt tgctacgcga ccgtcctcgc
     1321 caagttccgg cacgttattg aagaaagcgt acaggcggta gtgatcgcgc tgggatatgg
     1381 gatcgtattt atgatcgtga cagcgggcac attcgaaggt caggccaagc caggccatgc
     1441 cgagcgtgcg cacacggtcg gccacgtact cgacacggta ctcttcctcg atgatgccgc
     1501 cttccgagtt gatgacgtga ttgcgtccga aaccggtggc gattcgctgc tcaagtgtcg
     1561 agtttggcag gagatctccc gcaagctgtt ccgtgatgaa actgctataa ggcaggttcg
     1621 cgttgaagct gtcgatcacc cagtcgcgcc agcgccacat gctgcgctca gcgtcgttgt
     1681 tgaagccgtg cgtgtcggcg taacgcgcta cgtcaagcca gtcgagcgcc atacgctcgc
     1741 cgtaagccgg tgaggcgagc agccgatcga cggcggcgct gtacgctttc tcgccccgcg
     1801 ccttgacatc gcgcgcaaac gcatccgcat cgacaggcga tggcggcagg ccggtgagat
     1861 cgagcgacac acgccgcagc catgcgtccg gtgacgccgg agaactcggc cgcaacccct
     1921 ctgcctgcag ccgacggagg acgaacgcgt caatgggatt cctcgcccac tcgccaacgg
     1981 atggaacggg aggcttggac ggaggcacga aggcccagtg cttcgagtac tgaccgccgt
     2041 cacgg
//
LOCUS       DASPLT010001080         1194 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_73103, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001080 DASPLT010000000
VERSION     DASPLT010001080.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1194)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1194)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1194
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_73103"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(24..>1194)
                     /locus_tag="VEA63_11160"
     CDS             complement(24..>1194)
                     /locus_tag="VEA63_11160"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HYD84608.1"
                     /translation="ALLNYLDRLTITTMRDPLKADIAMTDAQFGLLTSAFLWIYGLCS
                     PLGGFLADRFSRRWVIVVSLFIWSAVTWLTGQMETFGGLLAARALMGVSEACYIPAAL
                     ALIADYHRGPTRSLATGLHMSGAYAGAALGGVGGIIAELHGWRFGFYLFGATGVVYSL
                     VVLALLRDAAPIPAPLETSAIKTDVTPRPSLRVAMSALFREPAFLLLLAVNALVGTAN
                     WGVYGWLPTYLREHFNLGLGEAGITATGYIQIASFIGVLIGGVWADRWSRTQPRARAW
                     VPAIGYCVVAPCLFLSVSTSLLPLAVAGLIVFGLGRGFFDANHMPILRSFSDERFSAT
                     GYGILNFVSCVTGGILIYVGGWLKDAQVDLSRVFQFSAVGLFAVGLMLFAMRRRV"
BASE COUNT          280 a          388 c          362 g          164 t
ORIGIN      
        1 gttccatttc gcgaagcacg cgctcaaacg cgtcgccgca tcgcgaagag catcaggcca
       61 acggcgaaca agccgaccgc ggaaaactga aacacccgcg agagatcgac ctgtgcgtcc
      121 ttcagccagc ctccgacata aatcaggatg ccgccggtca cgcagctcac gaaattaaga
      181 atgccgtagc cggtcgcgct gaaccgttcg tcggagaaag aacgcaggat cggcatgtga
      241 ttcgcgtcga aaaagccgcg tccgagtccg aagacgatca gtcctgcgac ggcgagcggt
      301 aacaggctcg tggatacgct aagaaaaagg cacggcgcga ccacgcaata gccgatcgca
      361 ggcacccacg cgcgggctcg cggctgagtg cggctccagc gatcggccca gacaccgccg
      421 atcaacacgc cgatgaacga cgcgatttga atgtatcccg tcgcggtgat gccggcttcg
      481 cccaaaccca ggttgaagtg ctctcgcaag taagtcggga gccagccgta aacgccccag
      541 ttcgcggtgc ccacgagagc gttcaccgcg aggagcagca gaaaagcggg ctcccgaaac
      601 aacgcggaca tcgcaacccg caaactcggc cgcggcgtga cgtcggtctt gattgcgctt
      661 gtttccagcg gcgcggggat gggcgcggcg tcgcgcagca gcgcgaggac cacaagcgag
      721 taaaccacgc ccgtggcgcc gaagagataa aagccgaaac gccatccatg gagttcggcg
      781 atgattccgc cgacaccgcc gagcgccgcg cccgcgtagg cgccactcat gtggagcccc
      841 gtggcgagcg atcgcgtcgg gccgcgatga tagtcggcga tcagcgcgag cgccgccgga
      901 atgtagcaag cttcgctcac ccccatcaac gcgcgcgccg ccaggaggcc gccgaacgtt
      961 tccatctgtc ccgtcaacca tgttaccgcc gaccagataa aaaggcttac cacgatcacc
     1021 catcggcgac tgaagcggtc cgccagaaaa ccgccaagcg gactgcagag accatagatc
     1081 caaagaaagg ccgacgtgag caggccgaac tgcgcatccg tcatcgcaat gtccgccttc
     1141 agcggatcgc gcatcgtggt gatcgtcagc cgatcgagat agtttagcag agcg
//
LOCUS       DASPLT010001081         2567 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_114282, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001081 DASPLT010000000
VERSION     DASPLT010001081.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 2567)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2567)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2567
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_114282"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..532
                     /locus_tag="VEA63_11165"
     CDS             <1..532
                     /locus_tag="VEA63_11165"
                     /inference="COORDINATES: protein motif:HMM:NF013630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LysM domain-containing protein"
                     /protein_id="HYD84609.1"
                     /translation="LSMDTISRENNSMLPVAGVVVGVLALLVGGYAAIKTSSLQKAVA
                     NQEEKVGRIDGLESQVSSVQAAVDKSSRDVNSLSRTTQDAFNTVAADLGNIHASITKL
                     EESAKRPAPAAAAAKGGSREPAVAGPDEYIVKGGDTGAKIARGQGVALADLIAVNPGV
                     EWTKLKPGQKVKLPKK"
     gene            complement(713..>2567)
                     /locus_tag="VEA63_11170"
     CDS             complement(713..>2567)
                     /locus_tag="VEA63_11170"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HYD84610.1"
                     /translation="LDEVVGKNPAHFMVGPETNPRTVARIRGAMARGRGISTDVVNYS
                     KSGRKYHLRLEIQPIRNETGELQNFIAIETDITARVETEQTLRRAKIEADDASRAKSE
                     FLASMSHEIRTPMNGVIGMTSLLMETQLNPEQRDFVNTIRTSGEALLTIINDILDFSK
                     IESGKLELEHMPFDLAVCIEEALELFALQASAKRLEIVYHIDSGVPAWVMGDVTRLRQ
                     VIVNLVNNAVKFTPTGSIAIEVKRLSAHLNQPGRFLLEFAVRDSGIGIPHDRLDRLFK
                     AFSQVDSSTTRKYGGTGLGLAISQRLSILMGGGIRVESTVGHGSSFIFTIATEAAALR
                     GEPILPAGLAHLRGAIVLCVEDHPVTQRRIRSILEPLGANCFFATNPAAAATLVPQLP
                     TRPQLVVIDGDETDGPSPLEALLHISAPRLLMLPFGQTAPAAPTDRQPFASLFKPFKT
                     ASFLHTLGFLFNPAHPTTSSSNTASGFSRKLSEEFPLDVLLAEDNAVNQKVALRFLER
                     LGYRADAVSNGIEAVTTLESRRYDLVLMDLQMPEMDGLEASRQIRARLAPESQPKIIA
                     LTANAMQGDRERCLAAGMDDYISKPVKLHEIETAIRRLFGKRESEMAASKR"
BASE COUNT          469 a          694 c          912 g          492 t
ORIGIN      
        1 tttatctatg gataccattt cgcgcgaaaa taacagcatg ctgcccgtgg ccggagtcgt
       61 ggtcggcgtg cttgctcttt tggtcggcgg ctatgccgcg atcaaaacct cgagcctcca
      121 gaaagctgtc gcgaaccagg aggaaaaggt gggccggatc gatgggctcg agtcgcaggt
      181 gagttccgtg caagcggcgg tggacaagtc ctcccgcgat gtgaactccc tttcgcgcac
      241 gacgcaggac gcgttcaaca cggtcgcggc cgatcttgga aatatccatg cttcgatcac
      301 gaagctcgaa gagtcggcca agcggccggc cccggcggcc gcagcggcca agggcgggtc
      361 tcgcgaaccg gcggtggctg gtccggatga atacatcgtc aagggtggcg ataccggagc
      421 gaagatcgca cgcggccagg gcgtggcgtt ggccgacctc atcgcggtta atcccggcgt
      481 cgagtggacg aagttgaagc cgggtcagaa ggtgaagctg ccaaagaagt aagcggcgca
      541 aacctgagtc cctttaagat cactcccggt ctcacgaccg ggagtttttt tgtctgcgct
      601 cacgaacgct cagccggacc catgcaggga aggtggagcg cgacgccctt ggcgcgctgt
      661 tacgggcgag cattatcaag cccgaggagg acgtcgggct ccatcttcca acctatcgct
      721 tggacgcggc catttcggat tcgcgtttac cgaagaggcg ccggatcgcg gtttcgattt
      781 cgtgcagctt caccggcttc gaaatgtagt cgtccatgcc ggcggcgagg cagcgttcgc
      841 ggtcgccttg catcgcgttc gcggtgagtg cgatgatctt cggctgagat tccggagcga
      901 ggcgcgcgcg gatctgtcgg ctggcttcga ggccgtccat ctccggcatt tgaaggtcca
      961 tcaagacaag atcgtagcga cgcgattcga gcgtggtgac ggcttcaatg ccgttgctga
     1021 cagcgtccgc gcggtagccg agacgttcga gaaaacggag ggcgactttt tgattaacgg
     1081 cgttgtcctc ggcgagaagc acgtcgagcg ggaattcctc ggagagcttg cggctgaagc
     1141 cgctggcggt gttcgagctg ctcgtcgtcg gatgcgcggg gttgaagagg aagccgagcg
     1201 tgtgaagaaa cgacgcggtt ttaaacggct tgaagagact ggcgaagggc tggcggtcgg
     1261 tcggtgcggc gggtgcagtt tgaccaaagg gcagcatgag gagccgcggc gccgagatgt
     1321 gcagaagcgc ttcgagcggt gaagggccgt cggtctcgtc gccatcaatt acgacgagtt
     1381 gcgggcgtgt cggtagctgg ggcacgagcg tggcggcggc ggcgggattg gtggcgaaga
     1441 aacagttcgc gccgagcggc tcgagaatgc tgcggatgcg gcgttgcgtg acgggatgat
     1501 cttcgacgca gagcacgatt gccccgcgca ggtgcgcgag gccggcgggc agaatcggtt
     1561 cgccgcgcag cgcggcggct tcggtggcga tggtgaagat gaacgaggag ccgtggccga
     1621 ccgtgctttc gacacggatg ccgccgccca tgagaatgct gagccgctgg gaaatcgcga
     1681 ggccgaggcc ggtgccgccg tatttgcgcg tggtcgacga atcgacctgg ctgaaggctt
     1741 tgaagagacg gtcgagccgg tcgtgaggga tgccgatgcc gctgtcgcgc acagcgaact
     1801 cgaggagaaa acgccccggc tgattcaaat gagccgacag gcgtttgacc tcgatcgcga
     1861 tgctgccggt cggggtgaac ttcacggcat tgttgacgag gttgacgatg acctgacgaa
     1921 ggcgcgtgac gtcgcccatg acccacgcgg gcactccgga atcgatgtgg taaacaatct
     1981 ccagccgttt tgcagacgcc tgcagtgcga acagctccag cgcttcctcg atgcagacgg
     2041 cgaggtcgaa cggcatgtgc tcgagctcga gtttgccgga ttcgattttc gagaaatcga
     2101 gaatgtcgtt gatgatcgtg agcagcgcct cgccagaggt gcggatggtg ttcacgaaat
     2161 cgcgctgctc gggattgagc tgcgtctcca tcaggaggct ggtcatgccg atgacgccgt
     2221 tcatcggcgt gcgaatttcg tgcgacatcg aagcgaggaa ctcgctcttg gcgcgcgagg
     2281 cgtcgtcggc ctcgatcttc gcgcgacgga gggtttgctc ggtttcgacg cgcgcggtga
     2341 tgtcggtctc gatcgcgatg aaattttgca gctctccggt ttcgttgcgg atcggctgaa
     2401 tctcgagacg caggtgatat ttgcggccgg acttggagta gttgacgacg tcggtgctga
     2461 tgccgcggcc gcgcgccatg gcgccgcgga tacgcgcgac ggtgcgggga ttggtctcgg
     2521 gaccgaccat gaaatgcgcg gggttcttgc cgacgacctc gtcgagc
//
LOCUS       DASPLT010001082         1189 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_278606, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001082 DASPLT010000000
VERSION     DASPLT010001082.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1189)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1189)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1189
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_278606"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1088)
                     /locus_tag="VEA63_11175"
     CDS             complement(<1..1088)
                     /locus_tag="VEA63_11175"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HYD84611.1"
                     /translation="MNPMLTDAELLHRYVDHRDQRAFIELVQRHFELVYGAALRRTGG
                     RTHLAAEIAQSVFTDLARKAATLRQHPTLVGWLYRSARYAANLALRAESRRDKLAQAL
                     TAMPDTSSPSDSAVDWERIRPLIDGAMDQLKERDREVMLLRHFSGLSFADVGSRLNIA
                     ENTARMRHERALGKLEAHLRVRGVTSTTAALSLLLANASFATAPAGLAASVSSAVLAS
                     APALAASAVSASVTTFIMHKLTVPIVFTVLASGLTVAVVSQHRKTSQLETTIAALRAN
                     RAAQDLKVEELTRLMSELLELEQEPATAPVAAPTSAPVVAAAPAKGPDEPGVTKKAPA
                     GWAKNGSKPDAYVVGVDRVNTLGGMSSA"
BASE COUNT          222 a          371 c          386 g          210 t
ORIGIN      
        1 tacgcgctgg acatgccgcc gagggtgttc acacgatcga cgccgaccac gtaagcgtcg
       61 ggcttggatc cgtttttggc ccaccccgcc ggcgcttttt tcgtgacgcc gggttcgtca
      121 ggtcctttcg ccggcgctgc ggcaacgacc ggcgcactcg ttggagcagc gaccggtgcc
      181 gtcgccggtt cttgctcgag ttccaacaat tcgctcatca gccgggtgag ctcttcgact
      241 ttgagatctt gcgccgcacg attggcccgc aacgcggcga tggtggtctc gagctggctg
      301 gttttccggt gttgggaaac cacggcgacc gtgagccccg aggcgagcac ggtgaaaaca
      361 atcgggacgg tgagtttatg catgatgaaa gtggtgacgg acgccgacac ggcggacgcg
      421 gccaacgcgg gggccgaggc cagcaccgcg ctgctcacac ttgcggcgag accggcggga
      481 gccgtggcga aggacgcgtt cgccaacagc aaactcaggg cagccgtggt cgacgtcaca
      541 ccgcggacgc gcagatgcgc ctccagtttt ccgagcgcac gctcgtggcg catgcgggct
      601 gtgttttccg cgatgttcaa cctactgccc acgtcggcaa acgacagccc gctgaaatga
      661 cggagcagca tcacctcgcg atcacgttcc ttcaactgat ccatcgcccc gtcgatgagg
      721 ggacggatgc gttcccaatc gactgcggaa tcggacggcg aagaagtgtc gggcatcgcg
      781 gtgagggctt gcgctaactt gtcccggcgg ctctccgccc gcagcgcgag attggcggcg
      841 tatcgagcac tccgatacaa ccagccgacc agcgttggat gctggcggag cgtcgcggct
      901 ttgcgggcga ggtccgtgaa aacgctctgg gcgatttcgg cggcaagatg ggttcggccg
      961 cccgtccgcc gcagcgccgc accatagacc agttcgaagt ggcgctgaac aagctcgata
     1021 aacgcccgtt gatcacgatg gtcgacgtag cgatgtaaca gctcggcatc ggtcaacatg
     1081 gggttcatcc atgtaacatc tcgccacgcc gaaatcgcac aaaaaatcca cgcgttgcac
     1141 gagcgcgtca tcttggagag ccaaggcagg cacggaggcc tgccgctac
//
LOCUS       DASPLT010001083         1138 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_136353, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001083 DASPLT010000000
VERSION     DASPLT010001083.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1138)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1138)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1138
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_136353"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..291
                     /locus_tag="VEA63_11180"
     CDS             <1..291
                     /locus_tag="VEA63_11180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD84612.1"
                     /translation="CHGEVIDGKAEIREDLDLSTDDAIRETLSEPGRLKEMIANDEMP
                     HEAKLSFRLRRNPQMQERLTKLKADYDANGEKEILLAWLKDVTATVTPKDEK"
     gene            complement(587..>1138)
                     /locus_tag="VEA63_11185"
     CDS             complement(587..>1138)
                     /locus_tag="VEA63_11185"
                     /inference="COORDINATES: protein motif:HMM:NF019222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1552 domain-containing protein"
                     /protein_id="HYD84613.1"
                     /translation="SVDPAVATPSGIPATQAEYVEVMYDMLALAFQTDSTRVATMIMG
                     HDGDNRSFSEIGITEGHHDLSHHQNNADRVAKVAMIDRWYVNQFSRFLQKLDTLQDID
                     GHSILHNSRILYGSGNADGNRHTHENLPLILAGGGGGLLSNGRYVQHGGKPLSNLFLS
                     LADQVGVRNFDRFGDSTGRLSDV"
BASE COUNT          250 a          353 c          327 g          208 t
ORIGIN      
        1 tgtcatggcg aagtcatcga tggcaaagcc gagatccgcg aagatctcga tctcagcacc
       61 gacgacgcca ttcgcgaaac gctttccgag cccggtcgcc tgaaggaaat gatcgcgaac
      121 gacgaaatgc cgcacgaggc aaagctctcg ttccgtttgc gccgcaatcc acagatgcag
      181 gaacgcctca cgaagctcaa agccgactac gacgccaacg gcgaaaaaga aatcctgctc
      241 gcctggctga aagacgtcac cgcgacagtg acgcccaagg acgaaaaata aaatcgatcg
      301 cgccccatcg cggtgccctc gtagcggcag gcctccgtgc ctgcctgctc gcggttcgga
      361 actgtagggg gtgcgctcgt cgcaccccgt tgcgtgcgag cgcgcaacct acgaaagcag
      421 acaggcacgg aggcctgccg ctaccctcaa gccgacgcgc ttaacccgca ttcatcacac
      481 gcagatgcgg gatgcgcgaa gtcggttgta gcgaaacgca tgagcgtttc gtccggcgag
      541 agacgaaacg ctcacgcctt tcactacaga tgtgaatttt ccgcgctcag acgtcgctca
      601 accggccagt cgaatcgcca aagcgatcga agttccgcac gccgacctga tccgcgaggc
      661 tcaggaacag attgctcaac ggcttgccgc cgtgttgcac gtaacggcca ttcgataaca
      721 gcccgccgcc gccacccgcc agaatgagcg gcagattttc gtgcgtgtgc cggttgccat
      781 ccgcattgcc gctgccgtaa agaatgcgcg agttgtgcag gatcgagtgg ccgtcgatgt
      841 cttgcagcgt gtcgagtttc tgcaggaagc gcgagaactg gttcacatac cagcgatcga
      901 tcatcgccac tttcgcgacg cgatcagcgt tgttttgatg atgcgaaaga tcgtgatgac
      961 cttccgtgat gccgatctcg ctgaacgaac gattgtcgcc gtcgtggccc atgatcatcg
     1021 tcgccacgcg cgtcgaatcc gtctgaaacg cgagcgcgag catgtcatac atcacttcga
     1081 cgtattcggc ctgcgtcgcg ggaattcccg agggtgtcgc gacggctgga tcgacgct
//
LOCUS       DASPLT010001084         1695 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_258807, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001084 DASPLT010000000
VERSION     DASPLT010001084.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1695)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1695)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1695
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_258807"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..746)
                     /locus_tag="VEA63_11190"
     CDS             complement(<1..746)
                     /locus_tag="VEA63_11190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HYD84614.1"
                     /translation="MPVAASAPSDSHERERERVFEVRQLTKTYGEGEAAVHALSGVDL
                     TLYRGELVVLLGPSGSGKSTLLNHLGGLDNPTSGTLLYHNRDLARADEPELTRYRRDA
                     VGFVFQFYNLIPSLTARENVALITEIAREPMAPEAALELVGLGPRMDHFPAQLSGGEQ
                     QRVAIARAIAKRPEVLLCDEPTGALDVNTGITVLEAIEHINRELGTLAVIITHNAILA
                     DMADRVFSMRDGRIVNERVNQTRDSVKNLK"
     gene            complement(807..1082)
                     /locus_tag="VEA63_11195"
     CDS             complement(807..1082)
                     /locus_tag="VEA63_11195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD84615.1"
                     /translation="MISTLLQLITRRPPADYDRNFVEEVRLLEHQPRRNPRVEKLIFV
                     CWLLIAAKCALVVWLVERYHMNFSPLWVNAPTIIFALMCTAAYFWRE"
     gene            1161..>1695
                     /locus_tag="VEA63_11200"
     CDS             1161..>1695
                     /locus_tag="VEA63_11200"
                     /inference="COORDINATES: protein motif:HMM:NF016836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HYD84616.1"
                     /translation="MPTAVYNAAAGCISRDDGDGPLAPKNLSVSASSTQDKQPTFGTS
                     GAKLAVRPVMSSSSTFLASRPAASLALRLACICGFALLALPGSALADAETDRKIERAA
                     QSSYNFRAILNNQVQVEANDGIVTLTGVVRDQQQKSLAEVTVRELPGVISVENKLETS
                     SPMHERADGWIALKINGL"
BASE COUNT          331 a          521 c          521 g          322 t
ORIGIN      
        1 cattttaaat tcttcaccga atcgcgcgtc tggttcacgc gctcgttgac gatgcggcca
       61 tcgcgcatcg agaacacgcg atccgccatg tcggcgagga tggcgttgtg cgtgatgatc
      121 acggcaagcg tgccgagctc acggttgatg tgctcgattg cctctaacac cgtgatgccg
      181 gtgttcacgt cgagcgcgcc ggtcggttcg tcacacaaca acacctccgg ccgcttcgcg
      241 atcgcgcgcg cgatggccac gcgctgttgt tcgccgccgg aaagctgtgc gggaaaatga
      301 tccatgcgcg gaccgagccc cacgagctcc aacgccgcct ccggcgccat cggctcgcgc
      361 gcgatctctg taatgagcgc aacgttttcg cgcgcggtga ggctcggaat caagttgtag
      421 aattgaaaca cgaaaccgac ggcatcgcgc cggtaacgcg taagctcggg ttcatcggcg
      481 cgggcgagat cccgattgtg gtagaggagg gtgccggagg tcgggttgtc gagtccgccg
      541 agatgattga gcagcgtgga tttgccactg cccgacggcc cgagcagcac cacgagttcg
      601 cctcgataca gcgtgagatc gacgccggag agcgcgtgca ccgccgcttc gccttcgccg
      661 taggttttcg tcagttggcg aacttcgaag acgcgctcgc gttcacgctc gtgcgaatcg
      721 gacggtgctg aggcggcaac gggcatgcga acgcgcacca aagccgagtc cttccgccgc
      781 agcaacctgc aaagccacgc cacccgtcac tcgcgccaga aatacgccgc ggtgcacatc
      841 agcgcgaaga tgattgtcgg cgcgttcacc cacagcggac tgaaattcat gtggtagcgc
      901 tcgaccagcc agaccacgag cgcgcacttc gccgcgatca gcagccagca aacaaaaatg
      961 agtttttcga cccgcggatt tcgacgcggc tggtgttcga gcaggcgcac ttcctccacg
     1021 aagttgcgat catagtccgc cggcggccgg cgcgtgatca attgcagcaa cgtggaaatc
     1081 atctgctgcg aacatagcag gcctgacgcg aaaggaaagc gagcgtgcca gcgcgccgcg
     1141 cttgttcatc caagaggatc gtgccgactg cggtttacaa cgcagccgcg ggttgcattt
     1201 cgcgggacga tggcgacggc cctcttgcac cgaaaaattt atctgtttca gcctcaagca
     1261 ctcaggacaa acagccgacg tttggcacca gcggcgctaa gttggcggtg cgcccagtca
     1321 tgtcttcttc gtccaccttc cttgcgtctc gtcctgccgc ttcgctcgct ttgcggctcg
     1381 cgtgcatctg cggctttgcc ctgttggcat tgccgggatc ggcgttggcc gacgcagaaa
     1441 cggaccggaa gattgagcgc gccgcgcagt cctcctataa tttccgcgcg atcttgaaca
     1501 atcaggtcca ggttgaggcg aacgacggca tcgtgaccct gaccggtgtc gttcgcgatc
     1561 agcagcagaa gtcgctcgcc gaagtcacgg tgcgtgagtt gcccggcgtc atttcggtgg
     1621 agaacaagct agagacatct tcgccgatgc acgagcgggc agacggctgg atcgcattaa
     1681 aaatcaacgg gctgt
//
LOCUS       DASPLT010001085         1437 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_93925, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001085 DASPLT010000000
VERSION     DASPLT010001085.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1437)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1437)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1437
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_93925"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..732
                     /locus_tag="VEA63_11205"
     CDS             <1..732
                     /locus_tag="VEA63_11205"
                     /inference="COORDINATES: protein motif:HMM:NF012345.4"
                     /note="Members of this family of hydrolases with an active
                     site Cys residue belong to MEROPS family C26; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HYD84617.1"
                     /translation="MLARKRIILIDAVPWSPDYPDTPLRSVDGWFRRHLGSIAEIELH
                     TLSADGDLAPATAPGVNGVILSGSPRDAWSNDPVNEKLCAVVRACQDRWIPFLGVCYG
                     HQILGRALDARVARHPEGWELGSTSVELTAAGQVSPLFDGLPSRFSVLSSHLDSVQTI
                     PKGCDLLARGAFTPIQSFHWQHRLFGVQFHPEMNPDIVRFLWAPRRERWRGHTSFDLD
                     RTLDEMPSAPLAPRILKNFAHHLLA"
     gene            729..>1437
                     /locus_tag="VEA63_11210"
     CDS             729..>1437
                     /locus_tag="VEA63_11210"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF012678.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HYD84618.1"
                     /translation="MTLTSFSFPTRTLFGAGAVRELPAHLAKLGLRRPLVVTDQGLFN
                     TDAFRALAHTLGPTEQDRTWFVFSGVHPNPVEQDVRDAADAFTANNCDSIIAIGGGSP
                     LDAGKAARLLVKRPQFDLSKFYDESDWSDLAPFIAIPTTAGTGSEVGRSSVITLDGTH
                     RKAVLFHAELLAKLVILDPELTRGLPPKLTAATGADALTHCIESYTCPVYHPMCDGIA
                     LEGVHLIVNALPRACRDG"
BASE COUNT          247 a          546 c          375 g          269 t
ORIGIN      
        1 atgctcgcgc gaaaacgcat catcctgatc gacgccgtgc cgtggtcgcc ggattacccg
       61 gatacacctc tgcgcagcgt ggatggctgg tttcgacgtc acctcggcag catcgccgaa
      121 atcgagttgc acacgcttag cgccgacggc gatctcgcgc ccgcgaccgc tccgggcgtg
      181 aacggcgtga tcctcagcgg ttcgccgcgc gatgcgtgga gcaacgaccc ggtcaacgag
      241 aaactctgcg ccgttgtccg cgcgtgccag gaccgttgga ttccgtttct tggcgtttgc
      301 tacggccacc aaattctcgg gcgcgccctc gacgcccgcg tcgcgcgtca tcccgaaggc
      361 tgggaactgg gctccacctc ggtcgaactc accgccgccg gccaagtctc gccactcttc
      421 gacggtcttc cgtcaagatt ctccgtcctg tcgagccacc tcgattccgt ccaaacaatc
      481 ccgaaaggct gcgatctgct tgcgcggggc gcatttactc cgatccagag cttccattgg
      541 cagcaccgcc ttttcggcgt gcagtttcat cccgaaatga atcccgacat cgtgcgcttc
      601 ctctgggcgc cgcgtcgtga acgctggcgt ggacacacct ccttcgatct cgatcgcacc
      661 ctcgatgaaa tgccgtcggc ccctcttgca ccccgcattc tgaagaattt cgcgcaccac
      721 ctcctcgcat gaccctcacc tccttctcct tccctactcg cacgctcttc ggcgccggcg
      781 ccgtccgcga gctacccgcc catctcgcca aactcggcct gcgacggccg ctcgtcgtca
      841 ccgatcaagg cctcttcaac accgacgcgt tccgcgcgct cgcccacacc ctcggtccaa
      901 ccgagcaaga tcgcacgtgg ttcgtcttct ccggcgtgca tccaaacccc gtcgagcaag
      961 acgtccgcga cgctgccgac gcattcacgg cgaacaactg cgacagcatc atcgccatcg
     1021 gcggcggcag cccgctcgac gccggtaaag ccgcgcggct actcgtgaag cgcccgcaat
     1081 tcgatctttc aaaattctac gacgaatcgg actggtccga cctcgcgcct ttcatcgcga
     1141 ttccgaccac cgccggaacc ggcagcgaag tcggacgcag ctccgtcatc actctcgatg
     1201 gcacgcaccg caaagccgtg ctgttccatg ctgaactgct cgcaaagctc gtcatcctcg
     1261 atcccgagct cacacgcggt ctgccgccaa aactcaccgc cgccaccggc gcagacgcgc
     1321 tcacgcactg catcgagagt tacacctgtc ccgtgtatca cccgatgtgt gacggcatcg
     1381 cgctcgaggg ggtccacctc atcgtcaatg cgcttccccg cgcctgccgc gacggca
//
LOCUS       DASPLT010001086         1976 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_114336, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001086 DASPLT010000000
VERSION     DASPLT010001086.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1976)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1976)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1976
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_114336"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..563)
                     /locus_tag="VEA63_11215"
     CDS             complement(<1..563)
                     /locus_tag="VEA63_11215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007364348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circularly permuted type 2 ATP-grasp protein"
                     /protein_id="HYD84619.1"
                     /translation="MSIFSSTYDSGNFYDETIADGGTVRPHYERLTERLRSLPVAEFN
                     QRRAAVDLAFLSRGVTFTVYGDNEGTERIFPFDLIPRIIPASEWAHIEAGLVQRITAL
                     NLFLHDLYHEQKILREGIVPASLVLGARNFRRELMNFNPPRGIYVHICGTDLIRDRDG
                     RYLVLEDNLRCPSGASYMIENRVAMRRA"
     gene            810..1241
                     /locus_tag="VEA63_11220"
     CDS             810..1241
                     /locus_tag="VEA63_11220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HYD84620.1"
                     /translation="MNSFLRFLQFNFIPRSADFALLVLRVWLGVSMLALHGWGKVTNF
                     GAMSSKFSDPIGVGPHMSLGLATFGEFLCAALLALGLTTRFAALVLAIVMATAFSTAH
                     GMKLSGPGSGELAFIYLAGFVTIFIAGPGRFSVDRKLGGAA"
     gene            complement(1354..>1976)
                     /locus_tag="VEA63_11225"
     CDS             complement(1354..>1976)
                     /locus_tag="VEA63_11225"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HYD84621.1"
                     /translation="RRYGGSGLGLAICSRLVQMMGGKLEVESTPGRGSVFYFSIPLPS
                     ADEQLPLPETSLPIETALGLEVLVAEDNDVNRRILASQLTRLGCRFTMVTNGEEALAA
                     LDSDAPPDVILMDCHMPKLDGWATTERLRRWSADPLPARRAAAALPIVALTAAALPEE
                     RTRCLAAGMNDFIAKPVKLAELQASLARFTRGSRVASEPPARAATE"
BASE COUNT          336 a          538 c          732 g          370 t
ORIGIN      
        1 gcgcgacgca tcgcgacgcg gttctcgatc atgtaagacg cgccggaggg acagcgaagg
       61 ttgtcctcga gcacgaggta acggccatcg cggtcgcgaa tgagatcggt gccgcagatg
      121 tggacgtaga tgccgcgggg cgggttgaag ttcatcaact cgcggcggaa gttacgcgcg
      181 ccgagaacaa gtgacgcggg cacgataccc tcgcggagaa tcttctgctc gtggtagaga
      241 tcgtgcagaa aaagattcag cgcggtgatg cgttggacga ggccggcctc gatgtgcgcc
      301 cactcggatg ccgggatgat gcgcgggatg aggtcgaacg gaaaaatgcg ctcggtgccc
      361 tcgttgtcgc cgtagacggt gaacgtgacg ccgcggctga ggaacgcgag atcgacggcg
      421 gcgcggcgct ggttgaactc tgcgacggga agtgagcgga ggcgctcggt gagacgctcg
      481 tagtgcggcc ggacggtccc gccatcggcg atggtttcgt cgtaaaagtt accggagtcg
      541 taagtggagc tgaagatgga cacgtgtggc gggcagggag cgggcggctg gatttagcag
      601 gagtcttgcc agcgcgggga gaggccgcgt cgggtcggat caatgcattt gagcgaggga
      661 tcgacgccga tgaacacgga ttcggatgag cgaccgaggg taagcggtgg ctcttcgacc
      721 aggtctcgtt aggagcaagc gatcgctaac cgcgcacgag gttgacggaa gttcgctcgt
      781 gcgcactttg acgcggtttt tcgctcttca tgaactcctt cctacgattc cttcaattta
      841 acttcattcc gcggagcgcg gacttcgcgc tgttggtgct gcgggtttgg ctcggtgtat
      901 ccatgctggc acttcatgga tggggcaaag tgaccaactt cggcgcgatg tcttcgaagt
      961 tttccgatcc gatcggcgtg gggccacaca tgagtctcgg gctcgccacg ttcggcgagt
     1021 tcttgtgcgc ggcgctgctg gcgctcgggc tgaccacgcg cttcgcggcg ctggtgctgg
     1081 cgatcgtcat ggcgacggcg tttagcaccg cgcatggcat gaagctgtca ggaccgggaa
     1141 gcggggaact ggcctttatt tatctggccg gattcgtgac gatcttcatc gcggggccgg
     1201 ggcggttttc ggtcgatcgc aagctcggcg gcgcggcgtg atctaggggc gttgcgcgga
     1261 gcgaaaagcg tgaatttttc gaggcgcagc cattgagacg gacgtgtcgc ttgcaaacag
     1321 cccgacgagg gacgtcgggc tccaccttgg cttttattcc gttgccgcgc gagccggcgg
     1381 ttcgctggca acgcgtgagc cgcgcgtgaa gcgcgcgagc gaggcctgga gctcggcgag
     1441 tttcacgggt tttgcgatga agtcgttcat gccggcggcg aggcagcgcg tgcgttcctc
     1501 gggcagcgca gcggcggtga gcgcaacgat cggcagcgcg gcggcggcgc ggcgcgcggg
     1561 gagcgggtcg gccgaccagc gtcgcagccg ctcggtcgtc gcccagccat cgagctttgg
     1621 catgtggcaa tccatgagga ttacgtcggg cggcgcgtcg ctatcgaggg cggcgagtgc
     1681 ttcctcgccg ttggtcacca tggtgaaacg acagccgagc cgcgtgagtt gagaggcgag
     1741 gatgcggcgg ttgacgtcgt tgtcctcggc gacgagcacc tcgagtccca gcgcggtttc
     1801 gatcggcagg ctggtctccg gaagcgggag ctgctcatcg gcggacggga gcggtatcga
     1861 gaaatagaat acggaaccgc ggccgggggt gctttcgacc tcgagctttc cgcccatcat
     1921 ctggacgagg cgcgagcaaa tcgccaggcc aaggccgctg ccgccgtaac gccgtg
//
LOCUS       DASPLT010001087         1033 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_237155, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001087 DASPLT010000000
VERSION     DASPLT010001087.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1033)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1033)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1033
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_237155"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1033
                     /locus_tag="VEA63_11230"
     CDS             <1..>1033
                     /locus_tag="VEA63_11230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009508709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HYD84622.1"
                     /translation="GAGKSTLLKILSRITEPTRGRIRLRGRVASLLEVGTGFHPELTG
                     RENIFLNGVILGMTRAETRRKFAEIVEFSGVEKFLDTPVKRYSSGMYVRLAFAVAAHL
                     EPEILIVDEVLAVGDAEFQAKCLGKMGDVATSGRTVLFVSHNMQAVTRLCSRCIYLER
                     GTVKMDGDANAVANTYLNSGGATSGRREWADLASAPGDDVARLHGIRIRSAADELIDA
                     TDVRDEVRVEMDYEVLKPGHQLLPHFAIRNQDGMVAFLSVDLDPQWRNKRRPTGRYIS
                     TARIPGNLLSEGLFSVQAVLMSINPQVVHTKIDDAVAFRVVDDLTASDTSRGDFRKPM
                     PGVLRPMLQ"
BASE COUNT          188 a          348 c          307 g          190 t
ORIGIN      
        1 acggcgctgg gaagtccacc ctgctcaaaa tcctcagccg gatcaccgag cccactcgcg
       61 ggcggatccg cctgcggggc cgggtcgcca gtctcctcga agttggcacc ggctttcacc
      121 ccgagctcac cggacgcgaa aacatttttc tcaacggtgt catcctcggg atgacgcggg
      181 ctgagacgcg ccgcaagttc gccgagatcg tcgaattctc gggtgtcgag aagttcctgg
      241 acacgcccgt gaaacgctac agcagcggca tgtatgtccg tttggcgttc gcggttgccg
      301 cgcacctcga acccgaaatc cttatcgtcg acgaagtcct cgccgtcggc gacgcggagt
      361 ttcaggcgaa atgtctcgga aagatgggcg acgtcgccac gtccggccgc accgtgctct
      421 tcgtttccca caatatgcag gcggtgaccc gcctgtgctc gcgttgtatt tacctggagc
      481 gcggcaccgt gaaaatggac ggcgatgcca acgcggtcgc aaacacctac ctcaactccg
      541 gcggtgcgac atcgggtcgc cgcgaatggg ccgacttggc cagcgcgccc ggcgatgacg
      601 ttgcccggtt gcacgggatc cgcatccgct ccgcggcgga cgagttgatc gatgccaccg
      661 acgttcggga cgaagtccgc gtcgaaatgg attacgaggt gctcaagccc ggacatcaac
      721 tgctgccgca cttcgccatc cggaaccagg acggcatggt cgcctttctc tccgtcgacc
      781 tggacccgca atggcgcaac aagcgccgcc cgacgggtcg ctatatcagc accgcgcgca
      841 ttcccggcaa tctgctgagc gaaggtctat tttcggtgca ggccgtcctc atgagcatca
      901 accctcaagt ggttcacacc aagatcgacg acgccgtcgc cttccgcgtt gtcgacgatc
      961 tcaccgcgag cgacacctcg cgcggtgatt ttcgcaagcc gatgcccggc gtcctgcgac
     1021 cgatgctcca gtg
//
LOCUS       DASPLT010001088         1682 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_177546, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001088 DASPLT010000000
VERSION     DASPLT010001088.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1682)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1682)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1682
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_177546"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            172..507
                     /locus_tag="VEA63_11235"
     CDS             172..507
                     /locus_tag="VEA63_11235"
                     /inference="COORDINATES: protein motif:HMM:NF013777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HYD84623.1"
                     /translation="MACTLDLIGDRWTLLLIRDLFTGKSRYGEFLESSEGIPTNVLAD
                     RLKRLESAGIIASEPYQENPPRYAYALTPKGAELKPVLGAVAQWGVRHIPKVKAHPSL
                     VGALEGSGR"
     gene            complement(556..1335)
                     /locus_tag="VEA63_11240"
     CDS             complement(556..1335)
                     /locus_tag="VEA63_11240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD84624.1"
                     /translation="MSPPNIVRQIDHVLLTTSKANELFELLTKTFQLPVVWPLSNYGS
                     FGSGGVALGNVNLEIIKRPAAAAGAASGGFVGLALEPESLRSSLAELDARGISHGAPA
                     PFRSLWTTVALPEVSSDALEIFLCDYTHDVAERRHRFLAQLQSRGGGPLSLHSVREIV
                     IGARDVKRTEAQWQKLLHPVRASADGAWPIGAGPAVRVVQAEHDEIQTVIVAVTSLEQ
                     ARRFLKQHDLLGADQPAALTLASSRFPGFNLTLVEQPVERR"
     gene            complement(1491..>1682)
                     /locus_tag="VEA63_11245"
     CDS             complement(1491..>1682)
                     /locus_tag="VEA63_11245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD84625.1"
                     /translation="ASVPERHTGNLRTGMLSFPLPFSTSEMTVSLLWHPRLDADPAHR
                     WLRAHVRDVCAGSAAVGRA"
BASE COUNT          325 a          501 c          550 g          306 t
ORIGIN      
        1 gagatcgtcg caacgagaag agtcatggga aacgctcgaa gatttgtgtc acttgcttta
       61 tgcaagttac accgcagtct cgtcacttgt cttttgcaag tataaaggtt tgatcgcgtc
      121 atgcccaagt cgtcatccgc accgaaagct tcccgccgct cctcgtgccc cgtggcttgc
      181 acgctcgacc tgatcggcga tcgctggacg ctgctgctca tccgcgatct cttcaccggc
      241 aaatcgcgtt acggggagtt tctggaatcg tccgaaggca ttccgacgaa cgttctcgcc
      301 gaccggctga aacggctcga gtccgccggc atcatcgcta gcgagccgta tcaggaaaac
      361 ccaccgcgtt acgcctacgc gctgacgccg aaaggcgcgg agttgaagcc ggtgcttggc
      421 gccgtcgcgc agtggggtgt gcggcacatt cccaaagtga aggctcaccc aagcctcgtc
      481 ggcgctctcg aaggcagcgg gcgctgagca gcgcaccgat tttgaatcag caatgcggaa
      541 gatcgcccgc gtttttcaac gtcgctccac aggctgctca accagcgtga ggttgaaacc
      601 gggaaagcgc gagctggcga gggtcagcgc cgcgggctgg tcggcaccga gcaggtcgtg
      661 ctgtttgagg aaacgtcgcg cctgctcgag tgaggtgacg gcgacgatca cggtttggat
      721 ctcgtcgtgc tcggcttgga caacgcggac cgcgggacct gccccgatcg gccacgcgcc
      781 gtcggcagag gcgcgaacgg ggtggagaag tttttgccac tgcgcttccg ttcgtttcac
      841 atcgcgcgcg ccgatgacga tctcgcggac ggaatgaagg gagagcgggc ctccaccgcg
      901 cgattgcagc tgcgcgagga agcgatgcct tcgttccgcg acatcatgcg tgtagtcgca
      961 gagaaaaatt tccagcgcat cgctcgaaac ctccggcagc gcgacggtgg tccacagcga
     1021 tctaaacggt gcgggcgcgc catgtgagat cccgcgggca tcgagctcgg ccaagctgct
     1081 ccgcagcgat tcgggctcga gcgcgagacc gacgaacccg ccgctcgcgg caccggcggc
     1141 agccgcgggg cgtttgatga tctccaggtt tacgttgccc aaagcgacgc cgccgctgcc
     1201 gaaacttccg tagttcgaca gcggccagac gacgggcagt tgaaacgttt tcgtgaggag
     1261 ttcgaacaac tcgttcgctt tgctcgtagt gagaagaacg tgatcgatct gccggacgat
     1321 gttcggtggt gacatggttg cgggagcggc acacatcgcc gacggtgcgc aacactcaac
     1381 gatcgcgcac atgaggatgg cgaatcgttt tgagagcagc gtgaccaggt tcattgcgac
     1441 atgcgagtgg gctcggtctg agtagcacgg ctcgagtgag tcgcaagcga tcaagcgcgt
     1501 ccgaccgcgg ctgatcccgc gcagacatcg cgcacgtgag cgcgcagcca gcgatgcgcg
     1561 ggatcggcgt ccaggcgcgg gtgccacagc agcgaaaccg tcatctcgga cgtcgagaag
     1621 ggaaggggaa aacttagcat tcccgtgcgc aggttgccgg tgtggcgttc tggaacgctc
     1681 gc
//
LOCUS       DASPLT010001089         2019 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_52587, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001089 DASPLT010000000
VERSION     DASPLT010001089.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 2019)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2019)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2019
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_52587"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..972
                     /locus_tag="VEA63_11250"
     CDS             <1..972
                     /locus_tag="VEA63_11250"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HYD84626.1"
                     /translation="VDYRRDEVTAGPDTAPRTHSEDGSVTGLFIQDDIAVTSDLTLHA
                     GARYDTYRLEDRLRQKFRHEGFSPNAGLTYAFTPRFSVNGSVATAYRGPDINDAFRID
                     LSQNDPNLDAEKARNYELRFLYREQRLQFEAGVYANRIKNIITNTLPWSRVYTNAGEL
                     ETDGLFARATYALPRAHFSLQYNHADTTLNGQTATRYQYSSLVSRIGDTWVADATWRP
                     LNQLDLGWNARLVQGLDDILVPFAITEIPDSRISKPGYVTHDFHLRWTPTFTDALTIT
                     LTVKNVFDKTYRSHGSLEDMTAFPGFAGVVGAPEPGRDLRLSAALRF"
     gene            1009..>2019
                     /locus_tag="VEA63_11255"
     CDS             1009..>2019
                     /locus_tag="VEA63_11255"
                     /inference="COORDINATES: protein motif:HMM:NF015863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY-associated TM helix domain-containing
                     protein"
                     /protein_id="HYD84627.1"
                     /translation="MPFRKIIFWLHLASGLIAGAIIAIMSVTGIAIAFEEEILAWIDR
                     KPAHIAAPAANARPKTIAELTALLRQTYPDLNVTTVVVPRDPQRAYEFYAGRDGPLYV
                     DPHTGLARESRAHAAHDFIHTLEEWHRWLGRPNGLTSTGRLVTGISNFAFLILCLTGL
                     WLWFPKLWNARALRPRLAFIKKFRGKARDFNWHHVFGFWSMPVLTLLAATGVVISFDW
                     AHRLVFTLAGEEAPKARNYGMMAVPLAVVPTPSPGTKPVSAEALLASVGVEFPDWQSL
                     HLTLASPSTNSAPLKPVSLGVTLPDAMPSRAYVPVELDPFTGEILQATRFQNRSAGLQ
                     ARV"
BASE COUNT          364 a          827 c          473 g          355 t
ORIGIN      
        1 gtggactatc gtcgcgacga agtcaccgcc ggccctgaca ccgctccgcg cacccacagc
       61 gaggacggct ccgtcaccgg gcttttcatc caggacgaca tcgccgtcac gtccgacctc
      121 acccttcacg ccggcgcccg ctacgacacc tatcgtctcg aagatcgcct ccgccaaaaa
      181 ttccggcacg agggtttcag ccccaacgcc ggcctcacct acgcgttcac ccctcgcttc
      241 agtgtcaacg gcagcgtcgc caccgcgtat cgcggacccg acatcaacga cgcgttccgc
      301 atcgatctct cacagaacga tccgaacctg gacgccgaga aagcccgcaa ctacgagctg
      361 cgttttctct accgcgagca gcgcctccag ttcgaagccg gggtctacgc caatcgtatc
      421 aagaatatca ttaccaacac gctcccctgg tcccgcgtct acacgaacgc cggcgaactc
      481 gaaaccgacg gcctcttcgc ccgcgcgact tacgcccttc cccgcgcgca cttcagcctc
      541 caatacaacc acgccgacac caccctcaac ggccagaccg ccacgcgcta ccaatacagc
      601 tcgctcgtct cccgcatcgg cgacacctgg gtcgccgacg ccacgtggcg cccgttgaac
      661 cagctcgatc tcggctggaa cgcccgactc gtccagggcc tcgacgatat cctcgttcct
      721 ttcgcgatca ccgaaatccc cgacagccgg atctccaaac ccggctacgt cacgcacgat
      781 tttcacctgc gctggacgcc caccttcacc gacgcgctca cgatcacgct caccgtcaaa
      841 aacgttttcg acaaaaccta ccgcagccac ggcagcctcg aagacatgac cgccttcccc
      901 ggtttcgccg gcgtcgtcgg tgcacccgaa cccggccgcg acctccgcct ctccgccgcc
      961 ctccgcttct agcggtaggt ggcgcgctcg ccgcgccact tcgccacgat gcccttccgc
     1021 aaaatcatct tctggctcca cctcgcgtcc ggcctcatcg ccggcgcgat catcgcgatc
     1081 atgtcggtca ccggcatcgc gatcgccttc gaagaagaaa ttctcgcgtg gattgatcgt
     1141 aagcccgcgc acatcgccgc gcccgccgcc aacgctcgac cgaaaacgat cgccgaactc
     1201 accgcgctcc tccgccaaac gtatccagat ttgaacgtca ccacggtcgt cgtcccgcgc
     1261 gatccgcagc gcgcctacga attctacgcc ggccgcgacg gcccgctcta cgtcgatcca
     1321 cacaccggcc tcgcccgcga atcccgcgct cacgccgcgc acgatttcat ccacacgctc
     1381 gaagaatggc accgctggct cggccgcccc aacggtctca cgtccaccgg ccgactcgtc
     1441 accggtatct cgaacttcgc tttccttatt ctctgcctca ccggcctctg gctctggttt
     1501 ccgaaactct ggaacgcccg cgcgcttcgc ccgcgcctcg ccttcattaa aaaattccgc
     1561 ggcaaagctc gcgacttcaa ctggcatcac gtcttcggtt tctggtcgat gccggtgctc
     1621 acgcttctcg ccgccaccgg cgtcgttatc tccttcgact gggcgcaccg actcgtcttc
     1681 accctcgccg gcgaagaagc tccgaaagcc cgcaactacg gcatgatggc cgtcccgctc
     1741 gccgtcgtcc ccactccgtc gcccggcacg aaacccgtat ccgccgaagc cctactcgcc
     1801 agcgtcggcg tcgaatttcc cgactggcaa tcccttcacc tcaccctcgc cagtccttca
     1861 accaattccg cgccgctcaa acccgtgagc ctcggtgtca cgctgcccga cgccatgccg
     1921 agtcgcgcct acgttcccgt cgaactcgat cctttcaccg gggagattct ccaagcgacg
     1981 cgcttccaaa atcgcagcgc cggccttcag gcccgcgtc
//
LOCUS       DASPLT010001090         1583 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_198141, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001090 DASPLT010000000
VERSION     DASPLT010001090.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1583)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1583)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1583
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_198141"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1154
                     /locus_tag="VEA63_11260"
     CDS             <1..1154
                     /locus_tag="VEA63_11260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD84628.1"
                     /translation="YQGKLDFHGNPLKAALAENKEVGLKLDLFDGRLSGTISRFEITR
                     TRAQVGSSSLFWFVPVASPNPKFDPSRDIVYVVNEQNPYTNTWNRTALDSAAQWDAAV
                     AAGSVYQATNSAGSTNWYVNASQSTGAAFLDAIFANAQKNNYDWWGWLYSGSDPGSLP
                     FDNLVNNATMDGNGAQNSVATGSDRSSGWDTQLIFTPNDNLQVLFSWSHINKVVLNAQ
                     TWAKYPYPQDRWAIWYAPYTFAATAGRPLEEVYTDPTDTSTFIAFGTGLALDDTPKDQ
                     GSIWINYQFDQDSALGGLSFGVGGVYESERSVYPAFGQNALDSEGNTIFLSTDSRTTF
                     NAMVKYDFKLRGRDASVQLNVENVADDQKLYGFIYAAPRRWQLTFSQRL"
     gene            1207..>1583
                     /locus_tag="VEA63_11265"
     CDS             1207..>1583
                     /locus_tag="VEA63_11265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD84629.1"
                     /translation="MNTDSIRRVEPRMDTNGHEWGGKRRWKTVVAVWGALAVWVGVVA
                     EPVKVELLEWAPGRFELLRAGEPYVVRGAGGEDLKSLAAAGANSVRTWGAEGIDGLLD
                     EAQRLGLTVTVGIWVEHERHGFD"
BASE COUNT          309 a          401 c          571 g          302 t
ORIGIN      
        1 attaccaggg gaaactcgat ttccacggca atccgctgaa ggcggcgctg gcggagaaca
       61 aggaggtggg attgaaactc gatctgttcg acggccggct gtcgggcacg atcagccgct
      121 tcgagatcac gcggacgcgc gcgcaggtgg gcagctcgag tttgttctgg ttcgtgccgg
      181 tcgcgtcgcc gaatccgaag ttcgatccga gtcgcgacat cgtttacgtc gtcaacgaac
      241 agaatcctta cacgaacacg tggaaccgga cggcgctgga ctcggcggcg caatgggatg
      301 cggcggtcgc ggccggatcg gtgtatcagg cgacgaattc cgcggggagc acgaactggt
      361 atgtgaacgc gtcgcagtcg acgggcgcag cgttcctcga cgcgattttc gcgaatgcgc
      421 agaagaacaa ctacgactgg tggggctggc tctatagcgg ttccgatccg ggcagcctgc
      481 cgttcgacaa tctggtgaac aacgcgacga tggacggcaa cggcgcgcag aacagcgtgg
      541 cgacgggcag cgatcgttcg tcgggctggg acacgcagct catcttcacg ccgaacgaca
      601 atctgcaggt gctcttctcc tggtcgcata tcaacaaggt ggtgttgaac gcgcagacct
      661 gggcgaagta tccctatcct caggacaggt gggcgatctg gtatgcgccg tatacgttcg
      721 cggcgacggc ggggcggccg ctcgaggaag tttacacgga tccgacggat acctcgacgt
      781 tcatcgcgtt cggcacggga ctggcgctcg atgatacgcc gaaagatcag ggcagcattt
      841 ggatcaacta tcagttcgat caggactcgg cgctgggcgg tttgtcgttc ggcgtcggcg
      901 gcgtttatga gagcgagcgg tcggtgtatc cggcgttcgg ccagaacgcg ctcgatagcg
      961 agggcaacac gatcttcctt tcgaccgaca gccgcacgac cttcaacgcg atggtgaaat
     1021 atgatttcaa gctgcgcggt cgcgacgcga gcgtgcaact caacgtggaa aacgttgcgg
     1081 acgatcaaaa gctttacggc ttcatctacg cggcgccgcg gcggtggcag ttgacgttta
     1141 gtcagcggct gtaggtgcgt gcgtttatcc aggcgcgaac cttgttgaaa ccacggatgg
     1201 acacggatga acacggattc gatccggagg gttgaaccac gaatggacac gaatggacac
     1261 gaatggggag ggaagcggcg gtggaaaacg gtcgtggcgg tttggggtgc gttggcggtg
     1321 tgggtcggtg tggtggcgga gccggtgaag gtcgagttgc tcgagtgggc gccagggcgg
     1381 tttgagttgt tgcgggcggg cgagccttac gtggtgcgcg gtgcgggtgg cgaagatttg
     1441 aaaagtctcg cggcggcggg cgcgaattcg gtgcgcacgt ggggagcgga aggaatcgat
     1501 gggttgctcg acgaagcgca gcggctcgga cttacggtga cggtcgggat ctgggtcgag
     1561 catgagcgtc acggtttcga tta
//
LOCUS       DASPLT010001091         1555 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_13721, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001091 DASPLT010000000
VERSION     DASPLT010001091.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1555)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1555)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1555
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_13721"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..714)
                     /locus_tag="VEA63_11270"
     CDS             complement(<1..714)
                     /locus_tag="VEA63_11270"
                     /inference="COORDINATES: protein motif:HMM:NF013706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HYD84630.1"
                     /translation="MRLARFGPHRHEKPALVDAQNRLRDASSLFRDWTPDFFQSEGLD
                     ALRRANLDTLPEVVPHARIGACVPRPGKLIGIGLNYSDHAAESGMPVPTEPVVFLKGT
                     NTVVGPHDDILIPRGSQKTDWEVELGVVIGREARYLASPADAVACIAGYCVSHDVSER
                     AFQLERGGQWTKGKSCDTFNPLGPWLVTADELPAPGSLDLRLWVNDELRQQGNTRTMI
                     FNVPFIVHYLSQLMTLEPGD"
     gene            complement(720..>1555)
                     /locus_tag="VEA63_11275"
     CDS             complement(720..>1555)
                     /locus_tag="VEA63_11275"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HYD84631.1"
                     /translation="ALITAGIPPEEAALIHAVEYNDLAAVETVARRTPLAALILEPVL
                     QNIGVVPPAPGYLEGLRRLADQYGFLLIFDEVKTGFRASLGGYQTLCGVAPDLSTWGK
                     AIANGVPLGAVAGKRNYLDLVLHDDPTKRVLLAGTYNAHPIPIAAGLATLKKLMDPSV
                     GVYSRLEHLGSVLQQGQEEISRRHGLRAVVSRLASASCVYFMDRLPAHWWDIATRHDS
                     AADKALRLSLIQRGIYQIPISAKQASISFAHTDEDIARTLEATEEAVRELKQTHPQAF
                     N"
BASE COUNT          272 a          421 c          585 g          277 t
ORIGIN      
        1 gtcgccgggc tcgagggtca tgagctgcga gagatagtga acgatgaacg gcacgttgaa
       61 gatcatcgtg cgggtgttgc cctgttggcg gagttcgtcg ttcacccaaa gtcgcaggtc
      121 gagcgagcca ggggcgggca attcgtcggc ggtgacgagc caggggccga gcgggttgaa
      181 ggtgtcgcag gacttgccct tggtccactg gccgccgcgt tcgagctgaa aagcgcgttc
      241 ggaaacgtcg tgcgagacgc agtagccggc gatgcaagcg accgcatcgg caggcgaggc
      301 gaggtagcgg gcttcgcggc cgatgacgac gccgagttcg acttcccaat cggttttttg
      361 cgaaccgcgc gggatgagga tgtcgtcatg cggaccgacg acggtgttcg tgcctttgag
      421 aaaaacgacg ggctcggtcg gcacgggcat gccggactcg gcggcgtggt cggaatagtt
      481 gaggccgatg ccgatgagct tgccggggcg agggacgcag gcgccgatac gcgcatgcgg
      541 aacgacttcg ggcagtgtat ccagatttgc acggcgcagc gcgtcgaggc cctcggattg
      601 gaagaagtcg ggcgtccagt cgcgaaagag cgaggacgcg tcgcgcaggc ggttctgggc
      661 gtcgacaaga gcgggttttt cgtgccgatg ggggccgaag cgagcgaggc gcatggcggt
      721 tagttgaagg cttgcgggtg ggtttgtttg agctcgcgca cggcctcttc ggttgcctcg
      781 agcgtgcgcg cgatgtcttc gtccgtatgc gcaaagctga tactcgcctg cttggcggaa
      841 attggaatct ggtagatgcc gcgttggatg agggagaggc gcagcgcttt gtcggcggcg
      901 gagtcgtgac gcgtggcgat gtcccaccaa tgcgcgggga ggcgatccat gaagtaaacg
      961 cagctcgcgc tcgcgaggcg tgagacgacg gcgcggagtc catggcggcg ggaaatctcc
     1021 tcctggcctt gctgcaaaac cgagccgagg tgctcgagac gtgaatacac gccgacggac
     1081 ggatccatga gcttcttgag cgtggcgagt ccggcggcta tcgggatcgg gtgcgcgttg
     1141 taggtgccgg caaggagcac gcgtttcgtc ggatcgtcgt ggagcaccag atcgaggtag
     1201 ttgcgtttgc cggcgacggc gccgagggga acgccattgg cgatggcctt gccccacgtc
     1261 gacaagtcgg gcgcgacacc gcacagcgtt tggtagccgc cgaggctcgc gcggaatccc
     1321 gttttcactt cgtcgaagat caggaggaaa ccatattggt cggcgagccg ccgcaggcct
     1381 tcgagatagc cgggcgcggg cggcaccacg ccgatattct gcagcacggg ttccaggatg
     1441 agcgccgcca gcggagttcg gcgggcaacc gtttcgaccg cagcgaggtc gttgtattcg
     1501 accgcgtgaa tgagcgccgc ttcctcaggc ggaattccgg cggtgatcag cgccc
//
LOCUS       DASPLT010001092         1035 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_278772, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001092 DASPLT010000000
VERSION     DASPLT010001092.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1035)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1035)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1035
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_278772"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            161..>1035
                     /locus_tag="VEA63_11280"
     CDS             161..>1035
                     /locus_tag="VEA63_11280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HYD84632.1"
                     /translation="MVVPALALAGLLATYAISQVSAADEQKSAAPAAPPAPKVTVASV
                     EQQLVTEYEDITGRVDATETVELRARVSGHLDAVRFQAGQLVRRGDVLFTIDPRWYRA
                     QFDLASAQVQQARAQFDIAEREAKRADELLAASAISSEEAESRRSRALQARAALASAN
                     AALASAQLDFEHTEVRAPIDGRIGRALVTAGNLVSGAPGNATLLATIVSQGEAFVYAD
                     IDENTLLKFNRLIRENRLASENGRIPVEMQLADEAGYPRRGYIESTDNRLDPATGSLQ
                     LRLVFANTDQALVPG"
BASE COUNT          177 a          375 c          301 g          182 t
ORIGIN      
        1 cggagtaaac ccgggcgcgg cggccgcgaa taagcgtcgc cgttctccgg gcctttttcg
       61 agccggcttc tttttccgta ttacacctca cacttcgttt tccactctca tgtccacgtc
      121 atccttacgc tcccttttac accgccgccg ccgtgccgtt gtggtcgtgc cagccctcgc
      181 gctggccggc ctgctcgcca cctacgccat cagccaggtc agcgcggccg atgaacagaa
      241 gtccgccgcg ccggccgctc cgccggctcc gaaagtcacc gtcgcctcgg tcgagcagca
      301 actcgtcacg gaatacgaag acatcaccgg ccgcgtcgac gccaccgaaa cggtcgagct
      361 tcgtgcccgc gtctccggcc atctggacgc ggtgcgtttt caagcgggac aactcgtgcg
      421 caggggcgac gtgctcttca caatcgatcc gcgctggtat cgtgcgcagt tcgacctcgc
      481 cagcgcgcaa gtccagcagg cacgcgcaca gttcgacatt gcagaacggg aagcgaagcg
      541 cgccgacgaa ctgctcgccg cctccgcgat ctcgagcgag gaagctgaat cacgtcgctc
      601 gcgtgcgctc caggcccgcg ccgcactcgc gtcggctaac gccgcgctcg ccagcgcgca
      661 gctcgatttc gaacacacgg aggtgcgtgc gccgatcgac ggacgcatcg gccgcgcgct
      721 tgtcaccgcc ggcaatctcg tttccggcgc gccgggcaac gcgacgctac tcgcgacgat
      781 cgtttcgcaa ggcgaggcgt tcgtctatgc cgacatcgac gagaacacgc tgctcaagtt
      841 caaccgcctg attcgcgaga accggctcgc ttccgaaaac ggccgcatcc ccgtcgaaat
      901 gcagctcgcg gacgaagccg ggtatccgcg ccgtggttac atcgagtcga ccgacaaccg
      961 cctggatccc gcgacaggtt cgctccagtt gagactcgtg ttcgcgaata ccgaccaagc
     1021 cttggtgccc ggttt
//
LOCUS       DASPLT010001093         2569 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_237263, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001093 DASPLT010000000
VERSION     DASPLT010001093.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 2569)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2569)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2569
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_237263"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..459
                     /locus_tag="VEA63_11285"
     CDS             <1..459
                     /locus_tag="VEA63_11285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HYD84633.1"
                     /translation="YRRPGLFMEITHDERLHFRWEGAFLALQKYLPKITAVPALRLKS
                     VTREDFEAWFKKYDPDVVLGHFPGVMTWMKSCGAKIPKTHAFVCLNTLRTENDCAALD
                     LRPALLGARAAELVIGQLLHNEFGIPEQPSLTTIPAILREGPTLNPKPAK"
     gene            complement(500..2083)
                     /locus_tag="VEA63_11290"
     CDS             complement(500..2083)
                     /locus_tag="VEA63_11290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonan acetylesterase"
                     /protein_id="HYD84634.1"
                     /translation="MAIAMWAGSLLGAAEQAGGEASAERAIPTVFIAGDSTAAKGNGE
                     TQEGWGVPLGDYFDSTKVNVVNRARGGRSSRTFVTEGLWEQLRAEVKAGDFVLIQFGH
                     NDGGAVNEEPPESTRPARARGSLLGIGEEMQEIDNVVTQRREVVHTFGWYLRKMIAEV
                     KARGATPVVLSLTVRNIWKDGKVERGSGDFRAWSRAVAEREGVAFIDATRIIADGYQQ
                     LGEARVAEFFPQDHTHTNATGADYNAVAVVAGLKGLRGEPWENFLSAKGAAVETDRIG
                     WLALPEPPNAALPTLFLIGDSTVRNGRGDGEGGQWGWGDPLVEHFDLAKINVVNRAVG
                     GLSSRTFLTQGYWDRVLALAKPGDFLLLQFGHNDSGPLNDTSRARGTITGVGEQTETI
                     DNLLTQQREVVGTYGSYLRRYVREAKARGITPIVCSPVPRKTWKDGKVARSAESYAGW
                     ARQVAEQEGVAFVDLNERIARRYEALGEARVNALFADAHTHTSWEGAKLNAAIVAEAL
                     RELEGAGLAEFLTKAETAN"
     gene            complement(2250..>2569)
                     /locus_tag="VEA63_11295"
     CDS             complement(2250..>2569)
                     /locus_tag="VEA63_11295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009508320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 95 protein"
                     /protein_id="HYD84635.1"
                     /translation="FGGAAAIAELLVQSRVNGDPSSGDAEIELLPALPPAWPDGEVRG
                     LRARGGFEVDVRWKGGELEQARIRSLRGTAATVKYRERRLELQATPKGRVLVLDGELK
                     ETE"
BASE COUNT          452 a         1029 c          620 g          468 t
ORIGIN      
        1 tatcgccggc cgggcctgtt catggaaatc acgcacgacg agcgcctgca tttccgttgg
       61 gaaggcgctt tcctcgccct gcaaaaatat ctcccgaaga tcaccgccgt tcccgcgctt
      121 cgactcaaat ccgtcacgcg cgaggacttc gaagcctggt tcaagaaata cgatcccgat
      181 gtcgtgctcg gtcattttcc cggagtgatg acgtggatga aatcctgcgg cgcgaaaatc
      241 cccaagacac acgccttcgt ctgcctcaac accttgcgca ccgaaaacga ctgcgccgcg
      301 ctcgacctcc gccccgcgct cctcggcgct cgcgccgccg agctcgtgat cggtcagctc
      361 ttgcacaacg aattcggcat cccggaacaa ccgtcgctga cgacgatccc cgccatcctc
      421 cgcgaaggcc cgacgctgaa ccccaagccc gcaaagtaat ttccgcgcga agaaagttcg
      481 gaaggtaggc gcctgctgat cagttcgccg tctccgcttt cgtcaaaaac tccgcgagcc
      541 ccgcgccttc caactcccgc aacgcctccg cgacaatcgc cgcgttcaac ttcgcccctt
      601 cccaactcgt gtgggtgtgc gcatccgcaa acaacgcatt cacccgcgct tcgccgagcg
      661 cctcataacg tcgcgcgatc cgctcgttaa gatcgacgaa cgcgacccct tcctgctccg
      721 ccacctgccg cgcccacccc gcgtaactct ccgccgatcg cgcgaccttt ccgtccttcc
      781 acgtctttcg cggcaccggc gaacacacga tcggcgtaat cccgcgcgcc ttcgcttccc
      841 gcacgtagcg tcgcaaatac gagccgtaag ttcccacgac ttcgcgctgc tgcgtcagca
      901 gattatcgat cgtctcggtt tgctccccca cgcctgtgat cgtcccgcgc gcccgcgacg
      961 tatcgttcaa tggcccgctg tcgttgtgcc caaactgcag cagcaaaaag tctcccggtt
     1021 tcgccagcgc gagcacgcga tcccaatacc cctgcgtcaa aaacgtccgg ctgctcaacc
     1081 cgcccacggc gcgattcacc acgttgatct tcgccaaatc gaagtgctcc accaacggat
     1141 cgccccagcc ccattgccca ccctcgccgt cgccgcggcc gttccgcacc gtcgagtcgc
     1201 cgatcaagaa cagcgtcggc agcgccgcgt tcggcggctc cggaagagcg agccagccga
     1261 tgcgatccgt ttcgaccgcc gcgcctttcg ccgagagaaa attttcccac ggctccccgc
     1321 gcaatccctt caacccggcc accaccgcca ccgcattgta gtccgctccg gtcgcattcg
     1381 tatgcgtatg atcctgcgga aaaaattccg ccaccctcgc ctcgccgagc tgctgataac
     1441 cgtccgcgat gatccgcgtc gcatcgataa acgccacgcc ctcccgctcg gcgaccgcac
     1501 gactccacgc gcgaaaatcg cccgacccgc gttccacttt tccatccttc cagatatttc
     1561 gcaccgtgag cgacaacaca accggagtcg cgcccctcgc cttcacctcc gcgatcatct
     1621 tgcgcaaata ccaaccaaac gtgtgcacca cctcgcgccg ctgcgtcacc acgttgtcga
     1681 tctcctgcat ttcctcgccg atccccagaa gcgaaccgcg cgcccgcgcc ggccgcgtcg
     1741 attcgggcgg ctcctcgttc accgcaccgc cgtcattgtg cccaaactga atcagcacaa
     1801 aatcgcccgc cttcacctcc gcgcgcagct gctcccacaa tccttccgtg acaaatgtcc
     1861 ggctgctgcg tccgccccgc gcgcgattaa cgacgttgac cttcgtcgaa tcgaaatagt
     1921 cccccagcgg cacgccccag ccttcctgcg tttccccatt ccccttggcc gccgtcgagt
     1981 cgcccgcgat gaacaccgtc ggtatcgccc gttccgcgct cgcctcgccg cccgcttgct
     2041 cagctgcgcc caacagcgag ccagcccaca tggctatcgc caccagtgac caccgtaacg
     2101 tgcgcgcccc cgtagcggca ggcctctgcg cctgcctgct ttcgtccgac gcactgacaa
     2161 gcaggcaggc acggaggcct gccgctactg cggatccctg aacgcgaact ttcaccgggg
     2221 tgtctctctt catgcttcaa acgttgcttt cattccgtct ccttcaactc cccgtccaac
     2281 acgagcacgc ggccttttgg cgtcgcctgc agttcaagcc tccgctcgcg atacttcacc
     2341 gtcgccgccg tgccacgcag actgcgaatc cttgcctgct cgagttcccc gcctttccag
     2401 cgcacgtcca cctcgaaccc gccgcgcgca cgcaacccgc gcacttcacc gtcgggccac
     2461 gccggcggca gcgccggcaa cagctcgatt tcggcgtcgc cgctggatgg gtcgccgttc
     2521 acccggctct gcaccaacag ctccgcgatc gccgccgcac cgccaaagt
//
LOCUS       DASPLT010001094         1906 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_258948, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001094 DASPLT010000000
VERSION     DASPLT010001094.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1906)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1906)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1906
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_258948"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..267)
                     /locus_tag="VEA63_11300"
     CDS             complement(<1..267)
                     /locus_tag="VEA63_11300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD84636.1"
                     /translation="MERFLRVAGDREKAAAVDLEQHFVSSFGFDYREMHRRLTRYVRS
                     GRFRAGAHPLPAVAASSTYARRAVPVDEITLRLAELALRVDRSAL"
     gene            complement(322..987)
                     /locus_tag="VEA63_11305"
     CDS             complement(322..987)
                     /locus_tag="VEA63_11305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD84637.1"
                     /translation="MSEVRRFSGLIAVVAMFVGVGSARLGASELPVADGKWSYYASPN
                     FELYSRNDERSSRELLYNLELLRAVFLERLNLKERRRVEVTVYYFRTKAEFQAYAPEA
                     MRKNDSFAGFHLFRPDRAVISMAPTEESHVARQIVFHEYVHHLFQIAEYDPPLWFNEG
                     TAEVLAGMKVERNRVLIGSPLEDRVLYLRQESLLPLNQLFAADQSSKAYADEKHTGVF
                     YAQ"
     gene            1351..>1906
                     /locus_tag="VEA63_11310"
     CDS             1351..>1906
                     /locus_tag="VEA63_11310"
                     /inference="COORDINATES: protein motif:HMM:NF012707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HYD84638.1"
                     /translation="MRRSPAVVYFLLLAFLAVAALSRAETLRLRADSWMPYNGDPTSE
                     LPGYAIEIARAILGPRNITLDYDNMSWGDALKAAAAGEIEAVIGANRQEAVGLIVPEN
                     PIGLPRIGLFAHKDNTSRYDNVPGLQKYKLGVILDYKYWDALDAYIEKNDAPQVIKFS
                     GEQPLEDAMVKLNAGEFDLLAETSS"
BASE COUNT          434 a          678 c          453 g          341 t
ORIGIN      
        1 caacgccgag cgatccaccc gcaaagcgag ttccgctagc cgaagcgtga tctcgtcaac
       61 cggcacggct cgccgcgcat aagtcgatga ggccgcaacc gccggaagcg gatgcgcccc
      121 cgctcgaaat cgcccgctcc gcacgtagcg cgtcaggcgt cgatgcatct cgcgatagtc
      181 gaacccgaag ctcgacacga aatgctgctc caagtccacc gcggccgctt tctcgcgatc
      241 ccccgccacc cgcagaaaac gctccaccgc ttccttcgga atcttcgaat cgccaaaatg
      301 ccagtagtgc agcagcgccc atcactgcgc ataaaacacc cccgtgtgct tctcatccgc
      361 atacgccttc gacgactgat ccgccgcaaa cagctggttc aacggcagca ggctctcctg
      421 cctgagatac aacacccgat cctccagcgg cgacccgatc aggactcgat tccgttccac
      481 tttcatcccc gccagcacct cagccgtccc ttcgttgaac cacagcggag gatcgtattc
      541 ggcaatctgg aaaagatggt gcacatactc gtgaaacacg atctgcctcg cgacatgcga
      601 ttcctccgtc ggcgccatcg aaatcaccgc ccggtccggc cgaaacaaat ggaagcccgc
      661 aaacgaatcg ttcttccgca tcgcctccgg cgcataggcc tgaaattccg ccttcgtccg
      721 gaaataataa accgtcactt cgaccctccg tcgttccttc agattcaaac gctccaggaa
      781 cacggcgcgt agcagctcca ggttatacaa aagctcgcgc gaagacctct cgtcgttgcg
      841 cgaatacagc tcgaaattcg gcgacgcata gtagctccac ttcccatctg ccaccggaag
      901 ctccgacgcc cccaaacgtg cactccctac gccaacgaac atcgcgacaa ccgcgatcaa
      961 acccgaaaac cgccgaactt cggacaaccg ccggagcaag aaagtcgacc gcaaaaaccg
     1021 ccgccaaagt tgagggtgaa acatggaaaa cgaaacacgc ccatcgaatt ctccccagca
     1081 cggtcctcaa tctcaaaagg caaatgtccg tcaaaaactt ctcgggacaa ttccttcaac
     1141 cgccgcagca tagcacccag ctgagaaata cgtgtcttcc acagcccgct tttaacgccg
     1201 gcggagagca cacgcttctt ctatccttct ccttgtgtgg cgcttcactg tcagaggtct
     1261 tatcgccgca tcgaacgccg tttccgctca cggtcgcgct ctcgtaatcg ctcctaaagc
     1321 ctccgagcgt ttctgccgat aaacgccggg atgcgtcgat ctcccgccgt tgtttacttt
     1381 ttgctgctcg cattcctcgc agtcgccgcc ctctctcgcg ccgaaacgct gcgcctccgc
     1441 gccgacagtt ggatgcccta taacggcgat cccacgtcgg aacttcctgg atacgccatc
     1501 gagattgccc gcgccatcct cggcccgcgc aacatcaccc tcgactacga caacatgtcg
     1561 tggggcgacg ccctcaaagc cgccgccgcg ggcgaaatcg aagccgtcat cggcgccaac
     1621 cgccaggaag ccgtcggcct gatcgtgccg gaaaatccca tcggcctgcc gcgcatcggt
     1681 ttgttcgcgc acaaggacaa cacctcccgc tacgacaacg tccccggtct tcagaaatac
     1741 aaactcgggg tgatcctcga ctacaaatac tgggacgcgc tcgacgccta tatcgaaaaa
     1801 aacgacgcgc cgcaggtgat caagttttcc ggcgaacagc cgctcgagga tgcgatggtg
     1861 aaactgaacg ccggcgaatt cgatcttctc gcggagacct cgtcgg
//
LOCUS       DASPLT010001095         1777 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_237282, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001095 DASPLT010000000
VERSION     DASPLT010001095.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1777)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1777)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1777
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_237282"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..516
                     /locus_tag="VEA63_11315"
     CDS             <1..516
                     /locus_tag="VEA63_11315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HYD84639.1"
                     /translation="EGLIQAYAEGFGFECAIFRFVSILGERYTHGHVFDFYQQLLEHP
                     DHLRVLGDGRQRKSYLYVQDCIDAVLHVARRHTARDAKHRTQIYNLGTPEYVEVNQSI
                     GFICETLGLNPRREYTGGDRGWIGDNPFIFLDTAKIRATGWQPKLGIRESILRTLRWL
                     EANRWVYEARV"
     gene            529..1398
                     /locus_tag="VEA63_11320"
     CDS             529..1398
                     /locus_tag="VEA63_11320"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HYD84640.1"
                     /translation="MPDYACLICHGPTHPAGRPRGRRTGREFVLQRCGSCGFVFVAEP
                     WTDYAQIYDVAYYVGRGSDPSVDYAYEFENPQRTVRRYEWRGLVRSIASLQPAPAKWL
                     DYGCGTGGLVRYARENSRYDMYGFDTGAWAERARASGLPIFSPAELPLHEGTFDIVTA
                     IEVIEHCVDPLNELRRLRRLLKPGGLLFLTTGNADSAPADFAAWKYVNPEIHVSYFTS
                     RALRLALEQTGFAPFDRGAIPGTNDILRFKILKSFRTRNVNLLERLLPWPILTRVADR
                     LVRFSATPLARAC"
     gene            1535..>1777
                     /locus_tag="VEA63_11325"
     CDS             1535..>1777
                     /locus_tag="VEA63_11325"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HYD84641.1"
                     /translation="MNLVVTGSSFGIGHALAERLLAHGHTVWGLARSEQTGLRTRHPS
                     TFFASRCDVSDWAEVSKTVAEITQRWPAIDGLVTCAG"
BASE COUNT          314 a          603 c          501 g          359 t
ORIGIN      
        1 gaaggattga tccaggcgta tgccgaaggg ttcggatttg agtgtgcgat ttttcgcttc
       61 gtctccatcc tcggcgaacg ctacacgcat gggcacgtgt tcgattttta tcagcaactt
      121 ttggagcacc ccgatcacct ccgcgtgctc ggcgacggac gacagcgcaa gagctacctc
      181 tacgttcagg attgcatcga cgccgttctc cacgtggccc gacgccacac cgcccgcgac
      241 gcgaagcacc gcacgcagat ctacaacctc ggcacgcccg agtatgtgga ggtcaaccaa
      301 tccatcggct ttatctgcga aacactcggc ctgaatccac gtcgtgaata cacgggcggc
      361 gatcgcggct ggatcggcga caatcccttc atcttcctcg acacggcgaa aattcgtgcc
      421 accgggtggc agccgaagct cggcattcgc gagagcatcc tgcgcacgct gcgctggctg
      481 gaggcgaatc gctgggttta cgaagcgcgc gtgtgacgcg ttgcaccgat gccagattac
      541 gcctgcctca tttgtcacgg tccgacgcat cccgcgggtc gcccgcgagg ccggcgcacc
      601 ggacgcgagt tcgttcttca acgctgcgga tcttgcggct tcgtcttcgt cgcagaacct
      661 tggacggatt acgcccaaat ctacgatgtc gcttactacg ttgggcgcgg gtccgatccc
      721 tcggtggact acgcctatga attcgaaaat ccgcagcgca cggtgcgccg ttacgaatgg
      781 cgcggactcg tccgcagcat tgcttccctt caacccgctc cggccaaatg gctcgactac
      841 ggctgcggca ccggcgggct cgtccgctac gcgcgcgaaa atagccgcta cgatatgtat
      901 ggtttcgaca cgggcgcgtg ggccgaacgc gcacgagcga gtggtttgcc gatttttagt
      961 ccggccgaac tccccttgca cgaaggcacg ttcgacatcg tcaccgcgat cgaggtcatc
     1021 gagcactgcg tcgatccgct caacgaactc cgccggcttc gtcgtctgct caaacctggc
     1081 gggctgcttt tcctcaccac cgggaacgcc gattccgctc ccgccgattt tgcggcatgg
     1141 aagtatgtca accccgagat tcacgtttcc tacttcacct cccgcgcgct tcgcctcgcg
     1201 ctggagcaaa cgggatttgc gccattcgac cgcggagcga ttcccggcac gaacgacatc
     1261 ctgcgtttca agatcctgaa aagctttcgc acgcgcaacg tgaacctcct cgagcgcctg
     1321 ctgccgtggc caatcctcac gcgagtcgcc gaccgcctcg ttcgcttcag cgcgacgccg
     1381 ctcgcccggg cgtgctgaag gcgcgatgcg cctctcgcaa actttcactg actccaaacg
     1441 cgcccgccgc acggattagt aggaactccc atccgtttct gccgtatctc caaatgcttt
     1501 acccgtcccg cggaacgccc tcttttgact gggcatgaat ctcgtcgtca ccggctccag
     1561 cttcgggatc ggccatgctc tcgccgagcg gctgctcgcg cacgggcaca ccgtgtgggg
     1621 cctcgcgcgt tccgagcaga cgggattgcg cacacgtcac ccttcgacgt tcttcgcctc
     1681 gcgttgcgac gtgagtgact gggcagaggt ctcgaaaacc gtcgcggaaa tcactcaacg
     1741 ttggcccgcc atcgacgggc tcgtcacatg cgctggc
//
LOCUS       DASPLT010001096         2674 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_258971, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001096 DASPLT010000000
VERSION     DASPLT010001096.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 2674)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2674)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2674
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_258971"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..777)
                     /locus_tag="VEA63_11330"
     CDS             complement(<1..777)
                     /locus_tag="VEA63_11330"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HYD84642.1"
                     /translation="MPPLLPVLLPVVIAAIVTGLLFAWWFPPTPPGAVAPMVRFTDVT
                     AEAGLLGWSSPESGETPTTLGGGVVCFDYDNDDHDDLLFVGGAPWPWEEGMAKRVSGR
                     SVALFRNDGSGKFRETTAMAGLNIELQGMSAAAGDFDNDGLTDVFVTGVGANHLFRNI
                     GRGRFEDVTESAGVIAAENTWSTGATWIDFDEDGKLDLVVAHYARWSREVDLRMAFVV
                     ANVGHSYGAPAGFIGMFPSVYRNVGDGRFALVPNSAGLRDV"
     gene            974..2062
                     /locus_tag="VEA63_11335"
     CDS             974..2062
                     /locus_tag="VEA63_11335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HYD84643.1"
                     /translation="MFRSSPAVRRLLGAFGFFALWSLIGLAFAGQFYLSSSLLGRSIT
                     WTQAISYSLADWYVWAVLSIPIARLARRFPPEANVPWRTAGVHLAAALGFALVYVGLR
                     SAVGLVHSWVIDEEVTFAEVFRPLFVKTFPFNLLIYGVIVSVSHALDYYRKYHERTVQ
                     ALELEKHLTEARLQSLLRQLKPHFLFNALNGISSLMYSDVAAADRMLIRLSELLRLTM
                     SQSGAPLTPLRTEIAFLECYLGIEQIRFRDRLSVQMEIDPLTNDAQVPSLILQPLVEN
                     AIRHGIEPQVRRGVITIRSSRSGDDLVLTVLDNGAGMPPDGFKREGIGIANSRARLAE
                     LYGAHQKFEMTNQPEGGLCVRLTFPFAT"
     gene            2300..>2674
                     /locus_tag="VEA63_11340"
     CDS             2300..>2674
                     /locus_tag="VEA63_11340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HYD84644.1"
                     /translation="MTKLRILIVDDEPLARERVRAMLRADPSAEIIGECGNGVDAIAT
                     IREQRPDVVFLDMQMPGADGLQVVNELPASERPAIIFVTAHDRFAVDAFGVHATDYLL
                     KPFDQERFKNALQRAAEHVRIRR"
BASE COUNT          496 a          997 c          672 g          509 t
ORIGIN      
        1 gacgtcgcgc aggcccgcgc tgttcggcac gagcgcaaac cgcccgtcac caacatttcg
       61 atacaccgaa ggaaacatgc cgatgaagcc cgccggcgcg ccatacgagt ggccgacgtt
      121 cgccacgacg aatgccattc gcaggtctac ctcgcgcgac cagcgcgcat agtgcgccac
      181 caccagatcc agtttcccgt cctcatcgaa gtcgatccac gtcgcacccg tgctccacgt
      241 gttctccgcc gcgatcacac cggccgactc ggtgacatcc tcgaatcgtc cacgtccgat
      301 gtttcgaaaa agatgattcg cgccgacgcc cgtcacgaac acatcggtga gtccgtcgtt
      361 gtcgaaatcc cctgcggccg cactcatgcc ctgcagctcg atgttcaacc ctgccatcgc
      421 cgtcgtctcg cgaaattttc ccgagccgtc gttgcgaaag agcgccacgc tgcgcccgct
      481 cactcgtttc gccatgcctt cctcccatgg ccacggagcg ccaccgacga acagcagatc
      541 gtcgtggtca tcgttgtcgt agtcgaagca caccaccccg ccgccgagcg tcgtgggcgt
      601 ttctccactc tccggcgaag accaaccaag caaacccgct tccgccgtca catccgtaaa
      661 ccgcaccatc ggcgccaccg cgccgggcgg tgtcggcgga aaccaccacg cgaataacag
      721 ccccgtcacg atcgccgcga tcaccacggg caacaacacc gggagcagcg gtggcacgac
      781 gcgcctcgtc tcgagcgaaa gcgccggtgc tcgaccgccc gcgttccatt cgacgcgcgc
      841 cgcccccgag ttccgccgcg cgtcggttgc gcgatcacgt gtttctctct gctcaatcat
      901 caggaagacg ttgcccaaag caccgcgaac cgattgaacc agcgcgcaac ccccgctgta
      961 aaaccttctc tcgatgtttc gttcgtcccc cgccgttcgc cgtcttctgg gagcgtttgg
     1021 ttttttcgca ctgtggtcgt tgatcggtct ggcgttcgcg ggccagttct atctctcgag
     1081 cagcctgctc ggccgttcaa tcacctggac ccaagccatc agttactcgc tcgccgactg
     1141 gtatgtatgg gccgtgctct cgattccgat cgcccggctc gcgcgccgct ttccgcccga
     1201 ggccaacgtc ccgtggcgca ccgccggcgt gcacctcgcc gccgcactcg gtttcgcgct
     1261 cgtctatgtg ggactccgct ccgccgtcgg tctcgttcac agctgggtca tcgacgagga
     1321 ggtgacgttc gccgaagttt tccgtccgct cttcgtcaaa acttttccgt tcaacctgct
     1381 catctacggc gtgatcgttt cggtcagcca cgcgctcgat tactaccgca aatatcacga
     1441 acgcaccgtg caggcgctcg aactcgaaaa acatctcacg gaagcgcgtc tccaatctct
     1501 cctccgccag ctcaagcccc actttctctt caacgcgctc aacggcatct cgtcgctcat
     1561 gtattccgac gtcgccgccg ccgatcgcat gctcatccgc ctttccgagc ttctgcgtct
     1621 caccatgtcg caatccggcg ctccgctcac tccactcaga accgagatcg cctttctcga
     1681 atgctacctc ggtatcgagc aaatccgctt ccgcgaccgg ctctccgtgc aaatggaaat
     1741 cgaccctctc accaacgacg cccaagtccc cagcctcatt ctccagcccc tcgtcgaaaa
     1801 cgccattcgc cacggcatcg agccgcaggt gcgacgcggc gtgatcacga ttcgctccag
     1861 ccgcagcggc gacgaccttg tcctcaccgt gctcgacaac ggcgccggca tgccgcccga
     1921 cggtttcaaa cgcgagggca tcggcatcgc caacagccgc gcccgcctcg ccgagcttta
     1981 cggcgcacac caaaagttcg agatgaccaa ccaacccgag ggtggacttt gcgtgcgcct
     2041 cacttttccc ttcgccactt agccggactc gtccagaaaa gaaaccacga atcgacacga
     2101 atgaacacga attcagagga gccgaaggac agctttgaga atcgttacgt cacccttcgg
     2161 tgttccgata aggctccgca ttcgaacgcg atttcattcg tgtcgattcg tgtccattcg
     2221 tggttcaaat gaattgttcc ggcttggagc ttcttcgccg gagcctgaac tcaggcgctc
     2281 accccacccc tgacgttcga tgaccaaact ccgcatcctc atcgtcgacg acgaaccgct
     2341 cgcgcgcgaa cgcgtgcgcg ccatgctccg cgccgacccc tccgccgaga tcatcggtga
     2401 atgtggcaac ggcgtcgacg cgatcgccac gattcgcgag cagcgcccgg acgtcgtgtt
     2461 cctcgacatg cagatgcccg gcgccgacgg ccttcaagtc gtcaacgaac tccccgcgag
     2521 cgaacgccca gccatcatct tcgtcaccgc acacgatcgc ttcgccgtcg acgccttcgg
     2581 cgtgcacgcc accgattacc tcctgaaacc cttcgatcag gagcgtttca aaaacgccct
     2641 ccaacgcgcc gccgaacacg tccgcatccg ccgc
//
LOCUS       DASPLT010001097         1548 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_258979, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001097 DASPLT010000000
VERSION     DASPLT010001097.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1548)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1548)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1548
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_258979"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1064)
                     /locus_tag="VEA63_11345"
     CDS             complement(<1..1064)
                     /locus_tag="VEA63_11345"
                     /inference="COORDINATES: protein motif:HMM:NF025010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HYD84645.1"
                     /translation="MISWIQRYFQHHFRIIFAVLLVGIIIAFVFTIGAAPGIGRADRQ
                     IVERRVFGYNLASAEDQARLFGDASLSAQLQTGYAPDGAEMQQYGLQRAASLVLADQL
                     HIPPTSQQEITDHIKSLRLFTNQEGQFDAQRYSMFRDSLKNNPRFNESTVSRVIADDV
                     RASKVQKLLAGPGYVLPADVSRQIEQADASWTLGVATIDYASYNPSIPTSDAILTKFY
                     EENTFRYEIPPRIVVSYVEFPALSLIGSINVSEAEVRAYYNANPARFPKPAAAEPNAA
                     PKIDPNADFAAVRPQVETTLKLERARRQAAKAAADFAFTLYDRKLVPNTPAFDEYLAA
                     EKLALKSLAPFTREGGPAEFG"
BASE COUNT          291 a          367 c          592 g          298 t
ORIGIN      
        1 ccgaactcgg cggggccgcc ttcgcgcgtg aaaggagcga gggatttcag cgcgagtttt
       61 tccgcggcga ggtattcgtc gaaagcgggc gtgttaggca cgagcttgcg atcgtagagc
      121 gtgaacgcga aatcggcggc ggctttggcg gcctgacggc gggcgcgttc gagcttgagg
      181 gtggtttcga cctgcgggcg cacggcggcg aaatcggcgt tgggatcgat tttgggtgcg
      241 gcgttgggct cggcggcggc gggcttgggg aagcgggcgg ggttcgcgtt atagtaggcg
      301 cgcacctcgg cttcggagac gttgatcgag ccgatgaggg agagcgcggg gaactcgacg
      361 tagctcacga cgattcgcgg cgggatctcg tagcgaaagg tgttttcctc gtaaaatttc
      421 gtgaggatgg cgtcgctggt cggaatcgac ggattgtagg aagcgtaatc gatggtggcg
      481 acgccgaggg tccagctcgc gtcggcttgc tcgatctggc ggctgacatc ggcgggaagc
      541 acgtagcccg ggccggcgag aagtttttgg accttggagg cgcgaacgtc gtcggcgatg
      601 acgcggctga cggtgctttc gttgaagcgc ggattgttct tcagcgagtc gcgaaacatc
      661 gaataacgct gggcgtcgaa ctggccttcc tggttggtga agaggcgcag ggatttgatg
      721 tgatcggtga tctcctgctg cgaggtgggc gggatgtgca gttggtcggc gaggacgagc
      781 gaggcggcgc gctggagacc gtattgctgc atctcggcgc cgtcgggcgc gtagccggtt
      841 tggagctggg cgctgaggga ggcgtcgcca aagaggcggg cctggtcttc ggcggaagcg
      901 agattgtagc cgaacacgcg tcgttcgacg atctggcggt cggcgcggcc gatgccgggc
      961 gcagcgccaa tcgtgaagac gaaggcgatg atgatgccca ccaacaagac ggcgaaaatg
     1021 atgcggaagt ggtgctgaaa atacctctga atccaggaga tcatggggga agaatcggcg
     1081 acggtaggtc gctgggatgg ggtggcaagg gcgaagtgcc gggcggcgga cggggcgtaa
     1141 agttggggat gaggccgcgc gctgagagcg agcggttagg tcaggtcggc cggaacaatt
     1201 catttgaacc acgaatggac acgaatcgac acgaatgaaa ccgagttcga gtgcgtcgtc
     1261 ttttcggaag accaaagggg gatgtcacga tcggccctga aagcttcctt cggctcctgt
     1321 gaattcgtgt tcgttcgtgt ccattcgtgg ttactcttct gcacgagtcc gatcagtcgt
     1381 cagaccggaa gaagaccgga acgtgggaca ccgtggccac ggctgtctgc actcgatggc
     1441 aacaaaggct tcgaaaaagt gcgatccaca tacgttagac gcccagcggt gttcggtaga
     1501 ttcgggggac gcgtttggtg acggagcaga gcgtttccca ggggatcg
//
LOCUS       DASPLT010001098         1133 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_198228, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001098 DASPLT010000000
VERSION     DASPLT010001098.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1133)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1133)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1133
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_198228"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..782)
                     /locus_tag="VEA63_11350"
     CDS             complement(<1..782)
                     /locus_tag="VEA63_11350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007363033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase domain-containing protein"
                     /protein_id="HYD84646.1"
                     /translation="MSGATLPMIFGREVQRIHCLGAGGMGVAPLAIYLSQLGFNVTGE
                     DDGLTEEVRGVLERERVRIVGMPKDCELVVYSSAIGKAHPVYAAAAARGIPLSRRGEM
                     LAEIVREKKLVAVCGSHGKTTTTALLITALRHANFSAGYLLGGLFADESSPARAGSNE
                     WVVAEIDESDGTIERFSPEITVCVNLDWDHPDQYRQFADLERTFAELFARTRGTVLVS
                     DAGALSGRLGVRQGQETPPDMVTFGRTGDFRGEIEQEGALGMT"
     gene            complement(934..>1133)
                     /locus_tag="VEA63_11355"
     CDS             complement(934..>1133)
                     /locus_tag="VEA63_11355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD84647.1"
                     /translation="EQTALSGLRAEVLDLIFNESLLRTFRSNLQRMDRENSLEFMLDD
                     LEEIAAGRSGSASRAEEAVVT"
BASE COUNT          220 a          449 c          257 g          207 t
ORIGIN      
        1 gtcattccca gcgcgccttc ctgctcgatc tcgccgcgaa aatctcccgt ccgcccaaac
       61 gtcaccatgt cgggcggggt ctcctgaccc tgccgcaccc ccagccgccc gctcaacgca
      121 cccgcatcgc tcaccagcac cgtcccgcgc gtccgcgcaa acagctccgc aaacgtccgc
      181 tccagatccg caaactgccg gtattggtcc ggatgatccc agtccaaatt cacgcacacc
      241 gtaatctccg gcgagaatcg ctcgatcgtt ccatcgctct cgtcgatctc cgcgaccacc
      301 cactcgttcg aacccgctcg cgccggcgaa ctctcgtccg cgaacaatcc gccgagcaga
      361 taccccgcgg aaaaattcgc gtgccgcagg gccgtgatca acagcgccgt cgtcgtcgtc
      421 tttccatgcg agccgcacac cgccaccaat ttcttttcgc gcacgatctc cgccagcatc
      481 tcgccgcgcc gcgacaacgg aatcccgcga gccgccgccg ccgcataaac cggatgcgcc
      541 ttcccgatcg ccgacgaata caccacgagc tcgcaatcct tcggcatgcc gacgatgcgc
      601 acccgttcac gctccaacac tccgcgcacc tcctccgtca gtccgtcgtc ctcgcccgtc
      661 acattaaaac ccagttgcga cagataaatc gcaagcggcg ccacacccat cccacccgca
      721 cccaaacaat ggatacgctg cacctcgcgc ccaaaaatca tcggcaatgt cgccccgctc
      781 atgcgcccgc cctgccttcc tcgttctcgt tcttcgttct ctttctcgtt ctctcgtcgt
      841 tcggaatcac cactccgaga gaacgagaac gataaagaga gagagaaaga gaaagatttt
      901 ccgagcgctt ccccctagcc ttggtggggt tcttcacgtc accaccgcct cctccgcccg
      961 gctcgcgctg ccgctccggc ccgccgcgat ctcttccaaa tcgtccagca tgaactcgag
     1021 cgagttctcg cgatccatgc gttgcagatt gctgcggaac gtccgcagca gcgattcgtt
     1081 gaaaatcaaa tcgagcactt cggcgcgcaa tccgctcagc gccgtctgct cga
//
LOCUS       DASPLT010001099         1172 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_114477, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001099 DASPLT010000000
VERSION     DASPLT010001099.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1172)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1172)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1172
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_114477"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            353..>1172
                     /locus_tag="VEA63_11360"
     CDS             353..>1172
                     /locus_tag="VEA63_11360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HYD84648.1"
                     /translation="MSYVPIATPGERNGQGGPTIATESFSRAASSGDDLPARREAKSP
                     QASAEDYSLIVHCHLCWDWVWQRPQQFLSRLSARHRILFVETVGPDPQLAAPYARFRE
                     AEGFPNITLLRLQFPTGQWSDGKFIDRERRRLVQEALKSELLAGQFQNPVQWFYDPMA
                     VTAFGGRMNEAATVYDCMDELSKFKGAPPVIVERERKLLALADVVFTGGRKMFEAKSR
                     FNANCHFYGCGVDVDHFGKARDESTRVPDDLAHLKKPVLGYFGVVDERMDYELVA"
BASE COUNT          234 a          305 c          407 g          226 t
ORIGIN      
        1 ccgaagcgaa cattgtgaag gttgcgcgat ccatatccgc ccgagtgctt gcgggcgact
       61 gacggcatac cgggacgggc gcgcaaaggc aaaatcgaag aggtaaaatc aggtgcgtgt
      121 tttcgacggt gaccggccgc cgtgcgcaag gcggcgtgct ccacgtcggc ttcgtttgga
      181 tcggagagcg gttgcaaatt gcacttgagc ccgggggagg cgttgcaagc agcacagcag
      241 aaaagagccg cgggaagcgt tcgccgatca cgagtcgcgg caatcgtttg caagtcggaa
      301 ggattgggaa ctaaccccgc tgctggcact gatatagcta aggagacgca ccatgtcata
      361 cgtgccgatt gcgacgccgg gagagaggaa cggtcaggga ggaccgacca ttgcgacgga
      421 gagtttttcg cgcgctgcat catccgggga tgatttaccg gcgcggcgcg aggcgaaatc
      481 gccgcaggcg tcggcggagg attactcgct catcgtccat tgtcatctct gctgggactg
      541 ggtgtggcag cggccgcagc aatttttgtc gcggctgagc gcgcggcacc ggattttgtt
      601 tgtggagacg gtggggccgg atccgcagtt ggcggcgccc tacgccaggt tcagggaggc
      661 ggaaggattt cccaatatca cgctgttgcg cctccagttc ccgaccgggc aatggagtga
      721 cggaaagttt atcgatcggg aacggcggcg gctcgtgcag gaggcgctta aaagcgagct
      781 gctcgccggc caatttcaga atcccgtgca gtggttttac gatccgatgg cggtgaccgc
      841 gttcggcgga cggatgaacg aggcggccac ggtttacgac tgcatggatg agctatcgaa
      901 gttcaaaggc gcgccgccgg tgatcgtcga gcgtgagcgg aagttgctgg cgctcgcaga
      961 cgtggtgttc accggcggcc gcaaaatgtt tgaagccaag agccgcttca acgccaactg
     1021 ccacttctat ggctgcggcg tggatgtgga ccatttcggt aaagctcgcg acgagtccac
     1081 ccgtgtgccc gacgacttag cgcatttgaa gaaacctgtg ttgggttact tcggggtcgt
     1141 ggacgagcgc atggattatg agctcgtcgc ga
//
LOCUS       DASPLT010001100         1926 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_198288, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001100 DASPLT010000000
VERSION     DASPLT010001100.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1926)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1926)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1926
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_198288"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            79..1140
                     /locus_tag="VEA63_11365"
     CDS             79..1140
                     /locus_tag="VEA63_11365"
                     /inference="COORDINATES: protein motif:HMM:NF012576.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HYD84649.1"
                     /translation="MPAAPVSTAPRRPTQKDIAALAGVHYSIVCRALQHHPGIPAVTR
                     ERVQQIAEEIGYRPDPALSALAAYRSSLRPPAFQGQLAWLCSTDAGVKWSQIDVYREY
                     MRSAAAQAARHGYNLLDYDLDSPSMTPQRLASIMQARNVRGILICPQPQRHATIDLPW
                     ENFSAVTLGHALTRPHLHVVSANHYHSTRCIFQKLRQLGYRRIGLAVPGEFNLRVGGD
                     FLAAYLLEQQSLPEKARLKPFLDPSTPLRSRFAAWLKANKPDALISTSYYYPPYLAQL
                     GVKIPDDLGVAILFRRVPHDDFAGIDQNPQQTAIVATNILVSMIQRNECGIAASAQHI
                     RVEGTWVDGFSVRQVGPPV"
     gene            complement(1260..>1926)
                     /locus_tag="VEA63_11370"
     CDS             complement(1260..>1926)
                     /locus_tag="VEA63_11370"
                     /inference="COORDINATES: protein motif:HMM:NF014841.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HYD84650.1"
                     /translation="AEYVFAQVILCLKQVWPQMLAVKANRGYVHGAHCPHGSYRSTVG
                     LIGLGHVGRLVAQRLKTLEVTTMAFDPYVGQAEAARLGVQLSSLDEVFAASNVVSCHV
                     PELPETAGLLCGRHFRAMRAGAAFINTARGSVVREGELIEVLRERPDLLALLDVTEPE
                     PPVPQSPLYELPNVLLTPHLAGSVGAECRRLGQCIVEDVERFARGERIEHEITRSAMA
                     HTA"
BASE COUNT          353 a          728 c          518 g          327 t
ORIGIN      
        1 tggtgcttct cttgcttcgg gaaacgtgtt cgctttctct cgtccgacca aatatcgaac
       61 ctgctgcctc tggtttccat gcccgctgcg cccgttagca ccgcgccccg tcgtccgacg
      121 cagaaggaca tcgccgctct cgcgggagtg cactattcga tcgtctgtcg cgcgctgcag
      181 caccaccccg gcattcccgc cgtcacgcgc gagcgggtgc agcagatcgc cgaagagatc
      241 ggctatcggc ccgatccggc tctctccgcc ctcgccgcct atcgcagctc gctgcggccc
      301 ccggctttcc aagggcaact cgcctggctt tgctcgacgg acgccggagt gaaatggagc
      361 cagatcgacg tctaccgcga atacatgcgg agcgccgcgg cccaagccgc gcgacatggc
      421 tacaacctgc tcgactacga tctggactcg ccctcgatga cgccccagcg cctggcgtcg
      481 atcatgcagg cgcgcaacgt ccgcggcatc ctcatctgtc cgcaacccca acggcacgcc
      541 accatcgatc tgccgtggga aaatttcagc gccgtcacgc tcggccacgc gctgacgcgc
      601 ccccatttgc acgtggtcag cgcgaatcac taccactcca cccgctgcat ctttcaaaaa
      661 ctgcgacaac tcggataccg tcgcatcggc ctggccgtgc cgggagaatt caatctccgc
      721 gtgggcggag acttcctcgc cgcctacctg ctcgagcagc agagccttcc cgagaaagcg
      781 cggctgaagc cgtttctgga tccgtccacg ccccttcgct cccgcttcgc cgcctggctg
      841 aaggccaaca aacctgacgc gctgatctcg accagttatt attacccgcc gtatctcgcc
      901 cagctcggcg tgaaaatccc ggacgatctg ggcgtcgcca tcctcttccg aagagttcct
      961 cacgacgact tcgccggcat cgaccagaac ccgcaacaaa ccgcgatcgt cgccacgaac
     1021 atcctcgtct cgatgatcca gcgcaacgag tgcggcattg ccgcgtccgc gcagcacatc
     1081 agagtcgaag gcacgtgggt cgacggcttt agcgtgcgtc aagtcggacc tccggtctag
     1141 ctgccctcgt ccgcccaacc gcgaggacgc gaacgccgaa cgccggacgc cgcaagcggt
     1201 ccccatcttc cgccgcagag tcgccggcga caagccgctc ccaccctgcg aaaatccgct
     1261 cacgccgtgt gcgccatcgc tgatcgagtg atctcgtgct caatgcgctc tccgcgtgcg
     1321 aaacgctcca catcctcgac gatgcactgc cccagccgtc gacactccgc acccacgctg
     1381 cccgccaggt gaggtgtcag caggacgttc ggcagctcgt aaagcggact ctgcggaacc
     1441 ggaggttcgg gctccgtcac atccaacaaa gcgagcagat ccggccgttc gcgcagcact
     1501 tcgatcagtt ctccttcgcg aaccacgctc ccgcgcgccg tgttgatgaa agccgctccc
     1561 gcccgcatcg cgcgaaagtg ccgcccgcac aacaaccccg ccgtttcggg caactccggc
     1621 acgtgacaac tgaccacgtt cgaagcggcg aacacctcgt ccagcgagct cagctggacg
     1681 cccagccgtg ccgcctcggc ctgcccgaca tacgggtcaa aggccatcgt cgtcacctcg
     1741 agcgtcttca gtcgctgcgc gaccagccgt ccgacgtgcc ccaacccgat cagacccacc
     1801 gtcgaacgat acgaaccgtg cgggcaatgc gcgccgtgca cgtaaccgcg attcgctttc
     1861 accgcgagca tctgtggcca gacctgcttc aggcaaagga tcacctgcgc gaaaacgtat
     1921 tccgcc
//
LOCUS       DASPLT010001101         1606 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_156366, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001101 DASPLT010000000
VERSION     DASPLT010001101.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1606)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1606)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1606
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_156366"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            320..1528
                     /locus_tag="VEA63_11375"
     CDS             320..1528
                     /locus_tag="VEA63_11375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD84651.1"
                     /translation="MSSALRISIGLNLVLAGVVVLLLWRDRLAAPAAVAPPPAFARAE
                     VVNASPRVEARKLQPGSAGGGFSPAAVAQLEQMGLSRRVVGSALLEEFNRRWDERLID
                     LERRHAPKRMAQRDYIEFARIRELEQIRELKEAFGEEGYRVWDKERTLRQLNSTGLPL
                     TAEEAEQAYRLQKDFDEKHKELQMAMEDGVADPADGATLQAQAQEALNRDLEQLFGKQ
                     RLDEMRGLTNPSAEVSWRYGDLSPTQEQTNAVLRTDGDYREGEAALVRRLNDHPATAA
                     NVAAELKALNDAREENLRGIFGAAAYEQSKRRNDPTYQTLQQYAGAWAIAETKIQPVY
                     DAVRILREQTDLTRAAAAMREAAGQPVNWREINAVIEQTRQQTETTLQTLVGDETLRR
                     LKQNGLLNSP"
BASE COUNT          335 a          456 c          562 g          253 t
ORIGIN      
        1 cgcagctcgg tggagccgac ccgaaaggcg gcgagcgggg ctccgcgcga atttggaaag
       61 gagggggctg gatgggcgcc gacttctgtg gccagccggc ctttcgagcc agctacgaag
      121 cccacaacaa aggcaacgat cagggctttc gcgtctgccg cagcaggtga tccgcacgat
      181 tgccggtggt gacgtctacg cgaacccgct catgccaaag atcggatcac gggagcgtgg
      241 gttgtagagc ggcgtttcgc agaggtgtgt ggcggtgtgc ccgcggccca agtctaaatc
      301 aacagaaagc gacggagtca tgtcatcggc attacgcata tccatcggtt taaatctcgt
      361 tctggccggt gtggtcgtgc tgctgctgtg gcgcgaccgc ctcgccgccc ccgccgccgt
      421 tgcgccgccg ccggcctttg cgcgagctga agtggtgaac gcaagtccgc gcgtggaggc
      481 caggaaactt cagcccgggt ctgccggtgg aggattttcg ccggccgcgg tggcgcagct
      541 cgagcaaatg ggactgtcgc gtcgggtggt gggcagcgcg ttgctcgaag agttcaaccg
      601 ccggtgggac gagcggctga tcgacctcga gaggcggcac gcgccgaagc ggatggcgca
      661 gcgcgactac atcgagttcg cacggatccg cgagctggag cagatccgcg agttgaagga
      721 agcattcggc gaggaaggtt atcgggtgtg ggacaaggaa cggacgcttc gccagctgaa
      781 cagcacgggc ctgccgctga ccgccgagga ggcggagcaa gcgtatcggt tgcagaagga
      841 tttcgacgag aaacacaagg agctgcagat ggcgatggag gatggcgtcg ccgatccggc
      901 cgacggtgcc acgctgcagg ctcaggccca ggaggccttg aaccgcgacc tcgaacaact
      961 attcggcaaa cagcgactcg acgagatgcg cggtttgacg aacccgagtg cggaagtgag
     1021 ctggagatac ggcgatctaa gtcccaccca ggagcagacg aacgcggtgc tgcggaccga
     1081 cggggattat cgcgagggcg aagcagcttt ggttcgccgg ctcaacgacc acccggcgac
     1141 cgcagcgaat gttgcggccg aattgaaagc gttgaacgat gcgcgggaag agaatttacg
     1201 cggtattttc ggggccgccg cttacgagca aagcaagcgg cggaacgatc ccacttacca
     1261 gacgctgcag caatatgccg gtgcgtgggc gatcgcagag acgaaaatcc agccggttta
     1321 cgatgcggtt cgcatccttc gcgaacaaac cgatctcacg cgcgccgccg ccgcgatgcg
     1381 cgaggcggcg gggcagccgg taaactggcg cgagatcaat gctgtgatag agcagacgcg
     1441 tcagcagacc gaaaccaccc tgcaaactct cgtcggcgac gagacactcc ggcggttgaa
     1501 acaaaacggt ctactgaact ccccgtgatc caaccgggga ggttgagttt gaaatctccg
     1561 ggtttaacgg ggaggcggag cgattgcgcc ggccatggcg gcatcg
//
LOCUS       DASPLT010001102         1200 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_156369, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001102 DASPLT010000000
VERSION     DASPLT010001102.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1200)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1200)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1200
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_156369"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1200
                     /locus_tag="VEA63_11380"
     CDS             <1..>1200
                     /locus_tag="VEA63_11380"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007359742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="isomerizing glutamine--fructose-6-phosphate
                     transaminase"
                     /protein_id="HYD84652.1"
                     /translation="SSDGRIALIHNGVIENYAQMKRFLLTKGYTFQSETDTEVLCNLI
                     AYHYAKGPAEQNGVSPFLESVRKALRHVEGTYGIVAMCVDFPGEIVAAREASPLILGV
                     GDGEYIIASDASALISRTQNVVYLKDGELVHVTPSTFSITTRDQSDVSPVVDKITWSI
                     ADAEKGQIAHFMEKEIFEQPAALENAMRGRFSADGSTAQFGGLNVTPSEFRQIERFMF
                     CACGTAWHACLVTEHLIERFARVPVEVDYASEFRYRNSPLDSNTLFFVMSQSGETIDT
                     LAALREAKRKGHRVLAVSNVVGSSIAREADGGIYQHVGPEIGVASTKAFTSQLLIGAM
                     IALYLGRMRDMSFSDGVQFVNALKGAPDLVRRVLQQAPHIREIARRYAKATDMLFLGR
                     LSLFPLAP"
BASE COUNT          225 a          384 c          365 g          226 t
ORIGIN      
        1 cagcagcgac ggccgaattg cgctcatcca caacggcgta atcgagaatt acgcgcagat
       61 gaagcggttt ctcctgacga agggttacac gtttcagtcg gaaaccgaca ccgaggtgct
      121 gtgcaatctg atcgcgtatc actacgcgaa aggaccggcg gagcagaatg gcgtcagccc
      181 gtttctcgaa agcgttcgca aggcgctgcg acacgtcgag ggcacttacg gcatcgtcgc
      241 catgtgcgtg gattttcccg gcgagatcgt cgccgcgcgc gaagcctcgc cgctgattct
      301 cggcgtcggc gatggcgaat acatcatcgc ctccgacgcg tcggcgctca tcagtcgcac
      361 gcagaacgtc gtctatctaa aagacggcga actcgtgcac gtcacgccat cgacgttttc
      421 catcacgacg cgcgaccagt ccgacgtttc gcccgtcgtc gacaagatta cctggtcgat
      481 cgccgacgcc gagaagggcc agatcgcgca tttcatggag aaggagattt tcgagcagcc
      541 cgccgcgctc gaaaacgcga tgcgcggccg cttctccgcc gatggcagca cggcgcaatt
      601 cggcgggctc aacgtcacgc cgagcgagtt ccgccagatc gagcgtttca tgttttgcgc
      661 atgcggcacc gcctggcacg catgtctcgt caccgagcac ctgatcgagc gtttcgcgcg
      721 cgtgccggtc gaggtcgatt acgcctcgga gttccgctac cggaattcac cgctcgattc
      781 gaacacgctc ttcttcgtga tgagccagtc cggcgaaacg atcgacacgc tcgcggcatt
      841 gcgcgaagcg aagcgcaagg gccatcgcgt gctcgccgtc agcaacgtcg tcggctccag
      901 catcgcgcgc gaagccgacg gcggcattta ccagcatgtc ggccccgaga tcggcgtcgc
      961 gtcgacgaag gcctttacgt cgcagctttt aatcggcgcg atgattgcgc tgtatctcgg
     1021 tcggatgcgc gacatgagtt tcagcgacgg cgtgcagttc gtgaacgcgt taaaaggcgc
     1081 gcccgacctc gtgcgccgcg tgttgcagca ggcgccgcac attcgcgaga tcgcgcgacg
     1141 ttacgcgaag gcgaccgaca tgctgtttct cgggcgactg tcgcttttcc cgctcgcgcc
//
LOCUS       DASPLT010001103         1645 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_177786, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001103 DASPLT010000000
VERSION     DASPLT010001103.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1645)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1645)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1645
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_177786"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            401..>1645
                     /locus_tag="VEA63_11385"
     CDS             401..>1645
                     /locus_tag="VEA63_11385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012377394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0035673 - oligopeptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006857 - oligopeptide transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide transporter, OPT family"
                     /protein_id="HYD84653.1"
                     /translation="MASPSAPLVEPFIAPETVMREFTIRAVLTGAVLGLIFGASSLYL
                     VLKVGLTVSASIPVAVISLAMFRALSKTGLRDATILENNITQTAGSAGESLAFGIGVT
                     MPAILILGFDLELTRVMLVAMLGGLLGILMMIPLRRALIVKEHAVLKYPEGTACAAVL
                     KAGANADDRAAASPAAQAEIRAAEAAGLGASPGAKIVFTGFGIGLAYKTLNVAFKGWK
                     DVSEKVFGAPLQSGSVAAEVSPELLGVGYVIGPRIGGIMAAGGVLAYLLLIPLIKFFG
                     QGTPGPIAPGTMPISEMGPGDIRGAYILYIGAGAVAAGGVISLIQSLPTIWHGLRAGL
                     RDVGQARNGNATNGGRLRTDRDISMKMVGIGIVVLLAGIMLAPSLHMNVVGALLIVLL
                     GFLFVTVSSRLTGEIGSTSNPIS"
BASE COUNT          266 a          470 c          559 g          350 t
ORIGIN      
        1 ggcggcgatt tcctgcaggc gaacggccag ccgatccaag tgagcggcat cagccaactg
       61 gtctttgccg ggggtatcag gtcgagcggc gctcccttgg acgcgttgac gaatcgcggg
      121 cagtttcgcc gcgacgggca aaatgtaacg tcgcagattg tcgtgaatcc atagtcacgg
      181 cgaccgttcg aggtgtgaag tgaagcccga cctgtggtcg ggcttcatgc ttttgagact
      241 gaggtgagtc agtgtgtgcg cgcgtctcga gcaggcgatg cgcgacagcc cgagggggat
      301 atcgggctcc cccaaaattt gagtgtgccg gtttgaaacc gatttcgcgt gttgaacccg
      361 gcggaaggcg cggaatactc cgcgcaactc aacgcacccc atggcttccc cctccgctcc
      421 gctcgtcgaa ccgttcatcg caccggagac ggtgatgcgc gagttcacca tccgcgccgt
      481 gctcaccggc gccgttctcg gcctgatctt cggcgcttcg tcgctttacc tcgtgttgaa
      541 ggtgggtctc accgtcagcg cttcgatccc ggtcgcggtc atctcgctcg cgatgtttcg
      601 cgcattgtcg aaaacgggtc tgcgcgacgc gacgattctg gaaaacaaca tcacgcagac
      661 ggcgggttcg gcgggcgaat cgctcgcgtt tggcatcggc gtgacgatgc cggcgattct
      721 tattctcggc tttgatctcg agctcacgcg cgtgatgcta gtggcgatgc tcggcggtct
      781 gctgggcatc ctcatgatga tcccgctgcg tcgcgcgttg atcgtgaagg aacacgccgt
      841 tctcaagtat cccgaaggca cggcgtgcgc ggcggtgttg aaagcgggcg cgaacgcgga
      901 tgaccgcgcg gcggcgtcgc cggcggctca ggcggagatt cgcgcggcgg aagcggcggg
      961 gctgggcgct tcgcccggag cgaagatcgt gttcaccggc ttcggcatcg gactcgctta
     1021 caagaccctc aacgtcgcgt tcaagggatg gaaggacgtt tcggagaaag tattcggcgc
     1081 cccactacag tcgggttccg tcgcggccga agtttcaccg gaattgctcg gcgtcggcta
     1141 tgtgatcggc ccgcggatcg gcggcatcat ggccgcgggt ggcgtgctgg cgtatttgct
     1201 cttgataccg ttgatcaagt ttttcggtca gggcacgccc gggccgatcg cgccgggcac
     1261 gatgccgatt tcggaaatgg ggccggggga tatccgtggc gcttacattc tctacatcgg
     1321 agcaggcgcc gtcgcggcgg gcggcgtcat cagtttgatc cagtcgctgc cgacaatctg
     1381 gcacggactg cgtgcgggtt tgcgcgacgt aggtcaggct cgaaatggca atgcgacaaa
     1441 cggcggtcgg ctgcgcaccg atcgcgatat ctcgatgaag atggtcggta tcggcatcgt
     1501 ggtgctgctc gcgggcatca tgctggcgcc gtcgttgcac atgaacgtgg tgggggcgtt
     1561 gttgatcgtg ctgctcggat ttttgttcgt gacggtctcg tcgcgtttga cgggagaaat
     1621 cggttctacg tccaatccga tttcg
//
LOCUS       DASPLT010001104         1011 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_259035, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001104 DASPLT010000000
VERSION     DASPLT010001104.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1011)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1011)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1011
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_259035"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            163..627
                     /locus_tag="VEA63_11390"
     CDS             163..627
                     /locus_tag="VEA63_11390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HYD84654.1"
                     /translation="MNIPAAREKFRKFLTQKGLRVTNQRLAIFDAALARTEHFTAEQL
                     LDYARTIDDSVSRATVYRTLPIMTESALLREVDIGSGEKFYHPNEEGGNTQIAQVVCL
                     DCDKIFEISAPFMSWYGNTVSSKLGLTPVSQRLQVSAHCDTLRLTGTCPRRS"
     gene            633..1001
                     /locus_tag="VEA63_11395"
     CDS             633..1001
                     /locus_tag="VEA63_11395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007364322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD84655.1"
                     /translation="MARPSDPEFEIKFFESVLKRDARYTEVIEILGGLYTKQGRIADG
                     LRMDRKLVRLLPANPTAHYNLACSLALVKRKSDALRSLRQAVELGYRDFDWMQQDPDL
                     EGLKNHPEFRALLDQLKPQS"
BASE COUNT          198 a          370 c          267 g          176 t
ORIGIN      
        1 cgatccccca caaggcaaga tgtcgccgca gcccgccgcc ccgcgcgggc ttggcgttcg
       61 gccgccgctg gaatctcacc ctccgcggct gggctggact ccgcctgacg cggcccccac
      121 gaacgaattg acacgcccga aacccgttcg gacagtgcgc gcgtgaacat ccccgccgcg
      181 cgcgaaaagt tcagaaaatt cctcacgcaa aaaggtctgc gcgtcaccaa ccagcggctc
      241 gcgatcttcg acgctgcgct cgcccggacg gaacacttta cggcggaaca gcttctcgac
      301 tacgcccgca cgatcgacga ttccgtctcg cgcgcgaccg tttaccgcac gctgccgatc
      361 atgaccgaga gcgctttgct gcgcgaagtc gacatcggct ccggcgaaaa attctaccat
      421 cccaacgaag aaggcggcaa cacgcagatc gcccaggtgg tctgcctcga ttgcgataaa
      481 attttcgaga tcagcgcgcc gttcatgagc tggtatggta acaccgtctc ttcgaaactc
      541 ggcctgacgc ccgtctcgca gcgcctccag gtcagcgctc actgcgatac gcttcgcctc
      601 accggcacct gcccgcgtcg ttcctgattt ccatggcccg cccttccgat cccgaattcg
      661 aaatcaagtt cttcgagtcc gtcctcaagc gcgacgcgcg ctacaccgag gtcatcgaaa
      721 tccttggcgg cctctacacg aagcaaggcc gcatcgccga cgggcttcgc atggaccgca
      781 agctcgtgcg tctccttccc gcgaatccca cggcgcacta caatctcgcg tgcagtctcg
      841 cgctggtgaa acgcaaaagc gacgccttgc gctcgctccg tcaggccgtg gagctcggct
      901 atcgcgactt cgattggatg cagcaggacc ccgatctcga ggggttgaaa aaccaccccg
      961 aattccgcgc cctcctcgat cagctcaaac cccagagctg acccgcccgt a
//
LOCUS       DASPLT010001105         1261 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_278878, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001105 DASPLT010000000
VERSION     DASPLT010001105.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1261)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1261)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1261
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_278878"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            151..>1261
                     /locus_tag="VEA63_11400"
     CDS             151..>1261
                     /locus_tag="VEA63_11400"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HYD84656.1"
                     /translation="MNRPGGHPHYQPQLLLNNGVTLLPSPAGALPSLRTSVGALAVAD
                     IDRNGMLDLFVGGRVIPGRYPLAPRSTLLKNEAGNFADATALADGLAEIGLVTSALFS
                     DVNDDGWPDLLVALEWGGVRYFQNLEGRGFRDCSDSSGFSAAGTGWWTSLAAADFNED
                     GRLDYVAGNVGLNTPYHATPAEPALLFYGEFEEKSQPQIVEAYHQNNKLYPRRGSQQL
                     PLAAPSIARRFTRVNDYARATLPELLDPKRLDAAQRFAATELRSGVFLSQPDGTHRFT
                     ALPRIAQIAPMQGIATGDFDGDGHADLYTVQNSYAPIPLVGRFHGGISQLLHGDGRGN
                     WTAVPPSDSNLVVSGDAKALASVDLDRNGWADFVVT"
BASE COUNT          241 a          401 c          370 g          249 t
ORIGIN      
        1 cccgcgcgag tcctgcgcgc tgcaggcgac gggacattcg agccgtgggc cggattcgcg
       61 actgcgctca acacgatcaa cgacggtcca attttaattt tcgatgcaaa cggcgacggg
      121 cgaaacgacc tgctcatcac aaaaggcggc gtgaatcgac cgggaggaca tccgcattac
      181 cagccgcaac tcttgctcaa caatggcgta accctgctcc cctcgcccgc tggcgcactt
      241 ccgtccctgc gcaccagcgt cggcgcgctg gcggtggcgg atatcgatcg aaacggcatg
      301 ctcgacctgt ttgtcggagg ccgtgttatc cccggacgct acccactcgc accgcgcagc
      361 acgctgttga agaatgaagc cggcaacttc gccgatgcga ccgcacttgc cgacggactg
      421 gctgagatcg gcctcgttac ttccgcgcta ttcagcgacg tgaacgacga tggctggccc
      481 gatctgctcg ttgcgctcga atggggcggc gtgcggtatt tccaaaatct tgaaggtcgc
      541 ggcttcagag actgttccga ctcgagcggt ttttctgctg ctggcaccgg ctggtggacc
      601 tcgctcgccg cggcggattt caacgaagac ggacgtctcg attacgtcgc cggaaatgtt
      661 ggactaaaca cgccttatca cgcgactccc gccgagcccg ctttgctgtt ttacggcgag
      721 ttcgaggaga agtcgcagcc acaaatcgtc gaagcctacc atcaaaacaa taaactgtat
      781 ccacgacgcg gatcgcagca gcttccgctc gcagcgccct cgatcgcgcg gcggtttacg
      841 cgcgtcaacg actacgcccg cgcgactttg cccgaactgc tcgatccgaa acgactcgat
      901 gccgcgcagc gttttgccgc aaccgaactg cgcagcggag tttttttgag tcagcctgat
      961 ggcacgcatc gtttcaccgc tctacctcga atcgcacaga ttgcgccgat gcagggtatc
     1021 gccacgggag atttcgacgg cgacggccac gccgatcttt acacggtcca aaactcctac
     1081 gctccgattc cgctggtcgg aaggtttcat ggcggcatta gccagttgtt gcacggagat
     1141 ggccgcggta actggaccgc ggtgccgccg tccgacagca atctcgtcgt gagcggcgat
     1201 gcgaaagcgc tcgcgtcggt cgatctcgat cggaatggct gggccgactt tgtggtgacg
     1261 c
//
LOCUS       DASPLT010001106         3421 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_136580, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001106 DASPLT010000000
VERSION     DASPLT010001106.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 3421)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3421)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3421
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_136580"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..997)
                     /locus_tag="VEA63_11405"
     CDS             complement(<1..997)
                     /locus_tag="VEA63_11405"
                     /inference="COORDINATES: protein motif:HMM:NF012363.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylesterase family protein"
                     /protein_id="HYD84657.1"
                     /translation="MSVRSGVTVAQGRLVGGAGEDPAITVFKGVPFAAPPVGASRWRP
                     PQPPASWSGVRKADTFGASAMQADQRSYGPWTEEYMFRNEASEDCLTLNVWTPAQNAE
                     ERRAVFVWIHGGAYNSGSGEVLLYDGEGLAKKGVVVVTFNYRLNVFGFLAHPELTAES
                     PHRASGNYGLMDQLAALRWVRENIAAFGGDRERITVGGQSAGAGALHHLLASPEARGL
                     FHRAIAQSGPWRRSATSLPLAEAEAQGAAFAATIGAPTLAELRGLTAAELFVRYEAQS
                     ARFRPIVDGWIVQEQVTAVHERGGQIDVPLLTGWTADEGSAQKGYGRSTVDEFTAR"
     gene            complement(1277..2284)
                     /locus_tag="VEA63_11410"
     CDS             complement(1277..2284)
                     /locus_tag="VEA63_11410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009512002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase"
                     /protein_id="HYD84658.1"
                     /translation="MTPAQLERARKLVCALHVRIRETLEAARAKQARSFAKIAAVTAA
                     DTIYQVDRLSEEAIVAWFDESWPRAWPVQIVMEGVDDDEALTFPRGTPVAETIWKCIV
                     DPIDGTRGLMYDKRSAWALTGLAPQRGTRTTLADIVVAVMTELPTSKQWRADQVSAVR
                     GCGARGVVARAQDVRTGKTRGFAVRPSQARDFKHGFASLARFFPEGKALTAEIEEALW
                     RELQGGATSTPLVFDDQYISTGGQLYELLVGHDRMLGDIRPRVFAKLGIASSLTCHPY
                     DICTELILREAGGIVEGLDGRALRDPLDTTSAVTWMGYANATLARQVRPVLRRLVKEY
                     L"
     gene            2817..>3421
                     /locus_tag="VEA63_11415"
     CDS             2817..>3421
                     /locus_tag="VEA63_11415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HYD84659.1"
                     /translation="MPAYVHAPAKPSRTAPTENLRRATPDAAIDDALVRRFAEGDESA
                     FVEIMERYRGKIFTVTLGLLRNHADAEEITQDTFIRAHRGLANFRGDSSLATWLYRIA
                     VNLARNRYWYFFRRRRQDSLSLDCALSEDSAATFADLVADVVQDPAQETVAGEFSTLV
                     DLCMEKLDPRHREILTLRNVLNRSYEEIATTLGINVGTVKSR"
BASE COUNT          633 a         1275 c          931 g          582 t
ORIGIN      
        1 accgcgccgt gaactcgtcc accgtcgacc ggccatagcc tttttgcgcg ctcccttcgt
       61 cggctgtcca gccggtgagg agcggcacgt cgatttggcc gccgcgttca tgcacggcgg
      121 tcacctgctc ctgcacgatc cagccgtcga caatcggccg gaaacgcgcg ctctgcgcct
      181 cgtagcgcac gaaaagttcc gccgccgtca acccccgcaa ctcggcgagt gtcggcgcgc
      241 cgatggttgc ggcgaacgcc gcgccttgtg cttccgcttc ggcgagcggc aggctcgtcg
      301 cggagcgccg ccagggtccg ctctgcgcga tcgcccggtg aaacagcccg cgcgcttcgg
      361 gcgatgcgag cagatggtgc aacgcgcctg cgccggcgga ttgcccaccc accgtgatgc
      421 gctcccgatc gccgccgaac gctgcgatgt tttcccgcac ccagcgcagc gcggcgagtt
      481 gatccatcag accgtagttg cccgaagcgc ggtgcggcga ctccgccgtg agttccggat
      541 gcgcgagaaa tccaaagaca ttgagacggt aattgaacgt cacgacgact acgcccttct
      601 tcgccaggcc ttccccgtcg tagagcaaca cctcgccgga accgctgttg taagctccac
      661 cgtgaatcca cacgaacacc gcccggcgct cctccgcgtt ctgcgccggc gtccagacgt
      721 tcagcgtgag acagtcctca ctcgcctcgt tgcgaaacat gtattcctcc gtccacgggc
      781 cgtaagaacg ctggtccgcc tgcatcgcgc tcgcgccaaa tgtatccgct tttcggacac
      841 cactccacga tgccggcggc tgcggcggtc gccagcgcga cgcgcccacc ggcggcgcgg
      901 cgaacgggac gcctttgaac accgtgatgg ccggatcctc acccgcgccg ccgacgagtc
      961 gtccttgcgc gaccgtgacg cccgagcgaa ccgacatcgc cgaatcggcg gcacccgcca
     1021 ccggtgacaa aaacaaaacc gagcaagcgc cggcgacccg cgcgcgggtg aatagggaat
     1081 ggggcatggc tgccatcaca cggcatcgca ccgcgccgcg caagttcgcg gcagtgcgaa
     1141 cgcgctgccg gacgcctcgc gctccgcggc cgtttgtcat acaatagatg acaaaccacc
     1201 ccacgcgctc tgcatcagca ttcacgattc acgaggctgc gaacgcgaag ctcgcgcgcg
     1261 ccgtcaccac cggcgcctac agatattctt ttaccaaccg tcgcagcacc ggccgcacct
     1321 gacgcgcgag cgtcgcattc gcataaccca tccacgtcac cgcggacgtc gtgtccagcg
     1381 gatcgcgcag ggcccggccg tccaacccct ccacaattcc gcccgcctcg cgcagaatca
     1441 gttccgtgca gatatcgtag gggtgacacg tcagcgacga cgcgattccg agctttgcaa
     1501 acacccgcgg tcgaatgtcg ccgagcatcc gatcatgccc gaccagcaat tcgtagagtt
     1561 gcccgcccgt gctgatatat tggtcgtcga acaccagcgg cgtgctcgtc gcgccgcctt
     1621 gcaactcgcg ccacaacgcc tcctcgatct ccgcggtcag agcttttcct tcgggaaaaa
     1681 atcgcgcgag tgacgcaaat ccatgtttga aatcgcgcgc ctgcgaaggc cgcaccgcaa
     1741 acccccgcgt cttccccgtg cgcacatcct gcgctcgcgc caccacgccg cgcgcgccgc
     1801 aaccgcgcac cgcgctcact tgatccgcgc gccattgctt cgacgtcggc aactccgtca
     1861 tcaccgcgac gacgatatcg gccagcgtcg tgcgcgtccc tcgctgtggg gccagccccg
     1921 tgagcgccca cgcgctgcgc ttgtcataca tcagcccacg cgtcccatcg atcgggtcga
     1981 cgatgcattt ccaaattgtc tccgccaccg gcgtgccgcg cggaaacgtc agcgcttcgt
     2041 cgtcgtcgac tccttccatc acgatctgca ccggccacgc acgcggccag ctctcgtcga
     2101 accacgccac gatcgcctcc tcactcaatc gatccacctg ataaatagta tcggctgccg
     2161 tcacagccgc gatcttcgca aaactccgcg cctgcttcgc ccgcgccgcc tcgagggttt
     2221 cgcgaatccg cacatgcagc gcgcacacca attttctcgc ccgttcaagc tgcgcaggag
     2281 tcatgacgcc cgagtgagca caccgcccgc gttttgccaa acgtagttcg ggttgttcga
     2341 ccgaaagaga tcactcgcgt gccgctcgct tcgccagaac gcattcgatg gcggagaact
     2401 cgacacgcgc ccgctcgcgc tcccggcgca ccagcaaggc cagcccgtat tcatcgcacg
     2461 caaatgcagg gtgcgcgcag tctgttgtag aaaaacgcgt gagcgtctcg tccgggcgag
     2521 agacggaacg ctcacgcgtt tcgcaccagg tgtgaatttt cccggccagc cgacgcccgc
     2581 gttgattcct ttcccacgtc aaatccccgc agcagcacga ggaaagatgg cggattgcgc
     2641 gaatcgcccc atcgccccct gcaatttgca cgagttttcg gcgaaaaatc gtatcgggca
     2701 ttccctgaac gaagtttatt caactgaccc ttagcgactt atcctcagca acgctccgct
     2761 ggcacaagtc gaggtataga cccgtcgctc aaccaaccaa ctcaaacttt cccactatgc
     2821 cagcctacgt tcacgccccc gccaagccca gccgcactgc gccgaccgaa aatcttcgtc
     2881 gcgccacgcc cgacgccgcg atcgacgacg cgctcgtgcg tcgtttcgcc gaaggcgacg
     2941 aatcggcctt cgtggaaatc atggaacgct atcgcggcaa gatcttcacc gtgacgctcg
     3001 gcctgcttcg caatcacgcc gacgccgagg agatcacgca ggatactttc atccgcgccc
     3061 atcgcggcct cgccaacttt cgcggcgatt cgtcgctcgc cacctggctc taccgcatcg
     3121 ccgtcaatct cgcgcgcaac cgctactggt atttcttccg ccgccgccgg caggactcgc
     3181 tctcgctgga ctgcgcgctc agcgaagaca gcgccgccac cttcgccgac ctcgttgccg
     3241 acgtcgttca agatcccgcg caggaaaccg tcgccggcga attctcgacg ctcgtcgatc
     3301 tctgcatgga gaaactcgac ccgcgccacc gcgaaatcct cacgctgcgc aacgtcctca
     3361 accgttccta cgaagaaatc gccaccacgc tcggcatcaa tgtcggcacc gtaaaaagcc
     3421 g
//
LOCUS       DASPLT010001107         1585 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_156413, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001107 DASPLT010000000
VERSION     DASPLT010001107.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1585)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1585)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1585
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_156413"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..269)
                     /locus_tag="VEA63_11420"
     CDS             complement(<1..269)
                     /locus_tag="VEA63_11420"
                     /inference="COORDINATES: protein motif:HMM:NF012314.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HYD84660.1"
                     /translation="MRRFIACGLLLLGSVVGVRASQGANIEEQVTALVAQPKVTLVHF
                     WAPWCSNCKREMRPDGWAKFVSENPDVTVVFINVWHGGLNGVRELS"
     gene            complement(342..704)
                     /locus_tag="VEA63_11425"
     CDS             complement(342..704)
                     /locus_tag="VEA63_11425"
                     /inference="COORDINATES: protein motif:HMM:NF015506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HYD84661.1"
                     /translation="MLHTHVVCRRLTAVVLITLGSSLLSAQSADFDERPVPVKAVSPV
                     YPEEMVKEKASAIVTVKLVIDENGDVLEQSITKSTRAEFEGAALAAVGKWKFKPARKA
                     GTAVRSVITLPIKFTYEG"
     gene            866..>1585
                     /locus_tag="VEA63_11430"
     CDS             866..>1585
                     /locus_tag="VEA63_11430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tex-like N-terminal domain-containing protein"
                     /protein_id="HYD84662.1"
                     /translation="MALPNPDHVLRLSQELNVKVFQVAATAQLLAEGATVPFIARYRK
                     EVTGELDEVQVLAIRDRLEQLNAIDERRASILASLKERNLLTPELEKAIAAADTLTAL
                     EDIYLPFRPKKRTRATIAKEKGLEPLADLVFAQDPATDPLAAAQAYVGREYVADDGKN
                     QKSKIESTDEALAGARDIIAERVSDDKDARARLRARYQAEAIVSSKVLIGKDTSPEAA
                     KFKDYFEWSEPLAKAPSHRILA"
BASE COUNT          317 a          588 c          405 g          275 t
ORIGIN      
        1 gacaattcgc gcacgccgtt aagcccgccg tgccagacgt tgatgaacac gacggtgaca
       61 tccggatttt cgctcacgaa tttcgcccag ccgtccgggc gcatctctcg tttgcagttc
      121 gagcaccacg gcgcccagaa atgcacgagc gtcacttttg gctgcgcgac gagcgccgtc
      181 acctgctcct cgatattcgc gccctgactc gcccgcaccc cgacgacgct tccgagcaac
      241 agcaacccgc acgcgatgaa tcttctcatg cgggcacgct aaaggcgcga ccggcaaaca
      301 ccagtcgcgc ctgcaaaaat tgtcgcgccc gcgcgcctcg ctcagccttc gtaggtgaac
      361 ttgatcggca gcgtgatcac cgaacgcacc gccgtgccgg ctttccgcgc cggcttgaat
      421 ttccatttgc ccaccgccgc gagcgccgcg ccttcaaact ccgcgcgcgt cgacttcgtg
      481 atcgattgct cgagcacgtc gccgttctcg tcgatcacca gcttcacggt cacgatagcg
      541 gaagccttct ccttcaccat ctcctccgga tacaccggag agaccgcttt caccggcacc
      601 gggcgctcgt caaagtcggc gctctgcgcg gagagcagcg agcttccgag cgtaattaaa
      661 acgacggcgg tcaaacgccg gcaaacaacg tgagtgtgaa gcatggatga aaatgccgcg
      721 aaaaattccg cagcggctcc cttttacggc acacgccgcc cgcacttgag ccccgcccgc
      781 gcttacgagc cgcgcttcat ccgtccatta gcgcgctttc cacttgctcg ttttcgcggt
      841 ccgtttccta tcggcctctt ttctcatggc ccttcccaat ccggaccacg tcctgcgcct
      901 ctcgcaggaa ctgaacgtca aagtttttca agtcgccgcg accgcgcagc tcctcgccga
      961 aggcgccacg gtccccttca tcgcccgcta ccgcaaggaa gtcaccggcg agctcgacga
     1021 ggttcaagtc ctcgccatcc gcgatcgcct cgagcaactc aacgccatcg acgaacgccg
     1081 cgcgtccatt ctcgcctcgt tgaaggaacg caacctcctc acgcccgagc ttgagaaagc
     1141 catcgccgcc gccgacacgc tcaccgctct cgaagacatc tatcttcctt tccgcccgaa
     1201 gaagcgcacc cgcgcgacca tcgcgaaaga gaagggcctc gaaccgctcg ccgatctggt
     1261 cttcgcccag gatcccgcca ccgacccgct cgccgccgca caagcgtatg tgggacgtga
     1321 atacgtcgcc gatgacggca agaatcagaa atcgaaaatc gagtcgaccg acgaagccct
     1381 cgccggcgcc cgcgacatca tcgccgaacg cgtgagcgac gacaaagacg cccgcgcccg
     1441 actgcgcgcg cgttatcaag ccgaggcgat cgtctcctcg aaagttctca tcggcaaaga
     1501 cacctcaccc gaagccgcca agttcaagga ctacttcgaa tggagcgagc cgctcgccaa
     1561 agccccttcg caccgcatcc tcgcc
//
LOCUS       DASPLT010001108         1129 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_198332, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001108 DASPLT010000000
VERSION     DASPLT010001108.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1129)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1129)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1129
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_198332"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1129
                     /locus_tag="VEA63_11435"
     CDS             <1..>1129
                     /locus_tag="VEA63_11435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3516 domain-containing protein"
                     /protein_id="HYD84663.1"
                     /translation="APVLCCTAEILANIALHRGDRSDIRAVVMDEFHYYSDVERGYAW
                     QVPLLAMPQARFLLMSATLGGTEFFEKDLERLTGAPGVTVRSDRRPVPLEFEYSETPL
                     TERVADLLKLNRAPVYLVYFTQRAASEAAQDLMSLNVSSKEEKAALQQELEGVKFNSP
                     YGKEVKRWLRHGIGVHHAGLLPKYRILVEKLAQKGLLKLICGTDTLGVGINVPIRTVV
                     FTQLWKYDGTKATILSVRDFRQVAGRAGRKGYDDKGYVIVQAPDHIIENKRAEERAAG
                     DPKKQKKLVKQRAPEGAVGWDAKTFERLMVAPAEGLQSRFDVSHGMMLLVLSREADGC
                     RALRQLIADSHETPHKKKALRRRAWQLFRALLERKIVEFIPP"
BASE COUNT          221 a          280 c          421 g          207 t
ORIGIN      
        1 cggcgccggt gttgtgctgc acggcggaaa ttctggcgaa catcgcgctg caccggggcg
       61 atcggagcga cattcgcgcg gtggtgatgg atgagtttca ttattactcg gacgtcgagc
      121 gcggctatgc atggcaggtg ccgttgctcg cgatgccgca agcgcggttt ttgctgatgt
      181 cggcgacgct cggtggaacg gagtttttcg agaaggatct cgagcgattg acgggcgcgc
      241 cgggggtgac cgtgcgcagc gatcggcggc cggtgccgct ggagttcgag tattccgaga
      301 cgccgctgac ggagcgcgtg gcggatttgc tgaagctgaa tcgggcgccg gtgtatctcg
      361 tgtatttcac gcagcgagcg gcgtcggagg cggcgcagga tttgatgagc ctcaatgtga
      421 gctcgaaaga ggaaaaggcg gcgctgcagc aggagttgga aggcgtgaag ttcaacagcc
      481 cctacggcaa ggaagtgaag cgctggctgc gtcacggcat cggcgtgcat cacgcgggat
      541 tgctgccgaa gtatcgcatc ctcgttgaaa agctcgcgca gaaagggctg ctcaaactga
      601 tctgcggaac ggacacgctt ggggttggaa tcaacgtgcc gattcgcacg gtcgtgttta
      661 cgcagttgtg gaaatacgac ggcaccaagg cgacgattct aagcgtgcgc gattttcggc
      721 aggtggccgg gcgcgcggga cgaaaaggct acgacgacaa aggctacgtg atcgtgcaag
      781 cgccggatca catcatcgaa aacaagcgcg cggaggagcg ggcggcgggc gatccgaaga
      841 agcagaaaaa gctcgtgaag caacgcgcgc cggagggcgc ggtcggctgg gatgcgaaga
      901 cgttcgagcg tttgatggtg gcgccggccg aagggctgca atcgcgtttc gatgtgtcgc
      961 acgggatgat gctgctggtg ttgagccgcg aggccgatgg ttgtcgcgcg ctgcggcagt
     1021 tgatcgcgga ttcgcacgaa acgccgcaca agaaaaaagc gctgcggcgg cgggcgtggc
     1081 agttgtttcg cgcgctgttg gagcggaaga tcgtcgagtt tattccgcc
//
LOCUS       DASPLT010001109         1658 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_237444, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001109 DASPLT010000000
VERSION     DASPLT010001109.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1658)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1658)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1658
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_237444"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            404..865
                     /locus_tag="VEA63_11440"
     CDS             404..865
                     /locus_tag="VEA63_11440"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HYD84664.1"
                     /translation="MARTNTPSNLEDHLGYWVRAVSNAVSQNFARKVEAKGVTVAEWV
                     FLRALYGAEGNSPTQLATRMGMTKSAISKLGERLAAKGLVVRTVKAPSSRRQTLTLTP
                     ASRRLVPALARLADQNDTEFFACLSVSERERLTKAMRRIASAHGLIGVPID"
     gene            904..1260
                     /locus_tag="VEA63_11445"
     CDS             904..1260
                     /locus_tag="VEA63_11445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD84665.1"
                     /translation="MNAPTTKVAQARLDAADKTASFPEIVGKLLEAGFEGYLVDRRRN
                     TTTYYLADGDKSCLKTVRRWSRSRALNQPAVSAQIRGRKPIRLNTQSSARTSTPMAAG
                     DILSLFQESACSTSPR"
     gene            1462..>1658
                     /locus_tag="VEA63_11450"
     CDS             1462..>1658
                     /locus_tag="VEA63_11450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD84666.1"
                     /translation="MCLTLSGWPVVFGVALAVLVSCAEPANQDAAQNGAARVNSGEGA
                     ATSMTPLTRGVADANLKWGPCP"
BASE COUNT          347 a          495 c          506 g          310 t
ORIGIN      
        1 agtcgcaacc ggctctcgct atcgcggagt ttgtcttgag cagttgcctc ctctgatcct
       61 ctctccgaca cgcttggtcc tcgcttcatg ggcgtgcaca ccaaatgtat ccaagtaagc
      121 tagtcaagtt cgcggcgccg ctaggtaatg gacggctcga cagaggacac gcgcacggaa
      181 ccggggacgt ctttgggcgt tgcttgaggt ttgtctgttg atcgtggagg taacgcgcgc
      241 gaattgggga gttcacccgg gaaagtccca cagatgccga caacgatcat cggcgctcaa
      301 ccgctaaacc acggactttc cgcctgctcg gcggcgcaaa caggaccagg atcgaaatag
      361 ttgactagga aaccgtctag gataggttcc tggtcgatct caaatggcgc gaaccaacac
      421 cccctcaaat ctcgaagacc acctgggcta ttgggtgcgc gcagtctcca acgccgtttc
      481 gcagaacttc gcccgcaagg ttgaggccaa aggcgtgacg gtcgccgagt gggttttcct
      541 acgcgcgctc tacggcgccg aaggcaattc gccaacgcag ctcgccacgc gtatgggcat
      601 gaccaaaagc gctatatcca aactgggcga acggctggcg gcaaaaggct tggtcgtgcg
      661 aacggtgaaa gcgccctcgt cccggcggca aacactgact ttaacgcccg cgagccggag
      721 acttgtcccc gctctggcgc gcctggccga tcagaatgac acggaattct tcgcgtgctt
      781 atccgtgagc gagcgtgaac ggctcacgaa agcaatgcga cgcatcgcat cagcgcatgg
      841 actcatcggc gtaccgattg actgaactca ttcaacacac tgaagacaca acaaggagag
      901 tgcgtgaatg cgccgacgac caaggtcgct caggcgcgcc tggacgcggc ggataagacc
      961 gccagctttc ccgagatcgt tggaaagctg cttgaagctg gttttgaggg ttatcttgtc
     1021 gatcgcaggc gcaatacgac gacctactat ctcgcggacg gcgacaaatc gtgcttgaaa
     1081 accgtgcgtc gctggagccg atcgcgcgcg ttaaatcagc cggcggtctc cgcacagatc
     1141 cgtggccgca agccaatccg cctgaatacg cagtcctccg cacgtacgtc aacgccaatg
     1201 gctgcgggcg atatcttgtc tctttttcag gaaagcgcgt gctctacttc gccacgatga
     1261 tggcgcaaaa gccgccgccg cggcgcgatg gacgcgcggg aggcagtagg tgaagccgca
     1321 ttttgcatcg ctgggggcgc gtcggccgga tgacacgggc agccgagcgg ctgatgggca
     1381 atggcgttca actagccgaa cgggcttagc ggggaagcgt tcggtcgcgg gataaagcaa
     1441 agttcccacc gcttcccgat cttgtgtctc acactctcgg gctggcccgt tgtgttcggt
     1501 gttgcacttg cagtgctcgt ttcctgcgcc gaacccgcaa atcaagacgc cgcacaaaat
     1561 ggcgcggcgc gggtcaattc cggcgaaggc gccgcgacga gcatgacgcc gctgacgcgc
     1621 ggagtcgctg atgcgaacct gaaatggggg ccgtgccc
//
LOCUS       DASPLT010001110         1372 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_259081, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001110 DASPLT010000000
VERSION     DASPLT010001110.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1372)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1372)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1372
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_259081"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..370
                     /locus_tag="VEA63_11455"
     CDS             <1..370
                     /locus_tag="VEA63_11455"
                     /inference="COORDINATES: protein motif:HMM:NF012497.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD84667.1"
                     /translation="TLAAIAPLLNGPTRITGIAHTRKQETDRVAGMARELQKLGQDVI
                     EENDALTITPRPLKSGVEIETYGDHRFAMSFAILGCHNLHADGRPWLSIKNPAVCAKT
                     FPHFFEVLAKVRSNSHPDQT"
     gene            502..1233
                     /locus_tag="VEA63_11460"
     CDS             502..1233
                     /locus_tag="VEA63_11460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HYD84668.1"
                     /translation="MTSSPFLIVAIDGGAASGKSTSSRVLSERFNLLHVDTGSFYRAV
                     TAELLRRGVAPTDHAAVKTALDALTFSTRVTGRSAAMEIGSRVVPDAEIRTPEVNASV
                     ALFAAIPELRAALLDYQRSQADTARRHGFRGLVMEGRDIGSKIFPDADLRFFLFADPE
                     ERARRREQQGLQDHVAKRDTLDRQRLEESLQGATLIDSTSLTLDQVVEQLSATIAAKF
                     ETVGGALRPDGAPAPLPKAPPASSP"
BASE COUNT          275 a          502 c          351 g          244 t
ORIGIN      
        1 cacgctcgcc gccatcgctc cgctgctcaa cggccccacg cgcatcaccg gcatcgcgca
       61 cacccgcaaa caggaaaccg atcgcgtcgc cggcatggcg cgcgagctcc agaaactcgg
      121 ccaggacgta atcgaggaaa acgatgcact cacgatcacg ccgcgcccgc tgaagagcgg
      181 cgtcgaaatc gaaacctacg gcgatcaccg cttcgcgatg agcttcgcca ttctcggctg
      241 tcacaacctt cacgccgacg gccgaccgtg gctctcgatc aaaaatcccg ccgtctgcgc
      301 caaaaccttc ccccacttct tcgaagtcct tgccaaagtc cggagcaatt cacatcccga
      361 ccaaacctaa aacgaagtga acgacgaatg gacacgaata gacacgaatg gaataccatt
      421 gaatcgctac ccggctccat ccgttccgct catcgtttct gctcttcgtg tgaattcgtg
      481 tccattcgtg gttaacaaaa agtgacctcc tctcccttcc tcatcgtcgc catcgacggc
      541 ggcgccgctt ccggtaaatc cacctcgtcg cgcgtgctca gcgaacgctt caacctgctc
      601 cacgtcgaca ccggttcgtt ctaccgcgcc gtcaccgccg agctcttgcg ccgaggcgtc
      661 gcgccgaccg atcacgccgc cgtgaaaacc gcgctcgacg cgctcacgtt ttccacgcgc
      721 gtcaccggcc gttccgccgc gatggaaatc ggcagccgcg tcgtccccga tgccgaaatc
      781 cgcacgcccg aggtcaacgc ttccgtcgca cttttcgccg cgattcccga gctccgcgcc
      841 gcactgctcg actaccaacg cagccaggcc gacacagcgc ggcgtcacgg ttttcgcggg
      901 ctcgtgatgg aaggccgcga catcggttct aaaatttttc ccgacgccga tctccgtttt
      961 ttcctcttcg ccgatccgga ggaacgcgcc cgccgacgcg aacagcaagg tttgcaagat
     1021 cacgtcgcca agcgcgacac gctcgaccgc cagcgcctcg aggaaagcct gcaaggcgcg
     1081 acgctgatcg acagcacctc gctcacgctc gatcaagtcg tcgaacaatt gtccgccacc
     1141 attgcggcga aattcgaaac ggtgggaggg gctttacgcc ccgacggcgc accggcaccc
     1201 ctgcctaaag ctccacccgc gtcatcgcca tgagccgcgc gttcccgcag gatcggatgg
     1261 agccgatcta cggttttttt cactacctga tcacggtcat ctacagtgcc gtatttcgcg
     1321 gcgaagtcgc gggccaggaa aacctcccgg agaacggcgc gttcctcgtc gc
//
LOCUS       DASPLT010001111         1167 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_259086, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001111 DASPLT010000000
VERSION     DASPLT010001111.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1167)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1167)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1167
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_259086"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(174..>1167)
                     /locus_tag="VEA63_11465"
     CDS             complement(174..>1167)
                     /locus_tag="VEA63_11465"
                     /inference="COORDINATES: protein motif:HMM:NF024126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase family protein"
                     /protein_id="HYD84669.1"
                     /translation="LRVGLFTPRSNTPVPVIIAPAGLPLGVTPLPRLPLGPTQGKGTN
                     ALLVVGPAQESSSGNSTNPPPPSAAQSPAASTADIAARHADLFRRGYAYLVFDHNDCG
                     EDTTLRLPDGSWAYRTTRFFPAYPDHDWGLLGAWAWGVSRIVDHLVTDPTINPAQIIV
                     TGFSRTGKSALIAGAFDERIALTAPVVTGGGGVGAYRFSGAGRGGKEGLGDMMRKYPN
                     WFSPHLRQFWGHVDQLPFDQHWFLALIAPRAFLALEGTTDRVSLLNAVKQTYLAARPV
                     YEFLGVPDRLGVNYADHGHAITPEDWNALLDFADVHLRGKKLDRRFDTFPAIDR"
BASE COUNT          224 a          298 c          453 g          192 t
ORIGIN      
        1 gggtgttcac cgagcttatg cccgaagagg cgccgacggc tgatccgcgg atcgagttgg
       61 tgcatcagga tttgacggcg gcgtgaacgg caaggcgcgc gttctgccgg aacgagtcgg
      121 tagtgaggcg cgagcaaccc gacgggacgt cgggttccat cttgcaggtg cacctagcga
      181 tcgattgcgg gaaaggtgtc gaagcggcgg tccagctttt tgccgcggag atggacgtcg
      241 gcgaagtcga ggagggcgtt ccaatcttcc ggcgtgatgg cgtgaccgtg gtcggcgtaa
      301 ttcacgccga ggcgatcggg gacgccgaga aattcgtaga cggggcgcgc ggcgagatag
      361 gtttgtttga cggcgttgag aagtgaaacg cggtcggtcg tgccttcgag agcgaggaac
      421 gcgcgcggtg cgatgagcgc gaggaaccaa tgttggtcga aagggagctg gtcgacgtgg
      481 ccccagaatt gtcgaagatg cggcgagaac cagttgggat atttacgcat catgtcgccg
      541 aggccctcct tgccgccgcg gcctgcgccg ctgaaacgat acgcgccgac gccgccaccg
      601 ccggtgacga cgggcgccgt gagcgcaatg cgttcgtcga aagcaccggc gatgagggcg
      661 gatttgccgg tgcgcgaaaa gccggtcacg atgatttgcg ccggattgat ggtggggtcg
      721 gtgacgaggt gatcgacgat tcgagacacg ccccaagccc aggcgccgag gagaccccaa
      781 tcgtgatcag gatacgcagg aaaaaagcgg gtggtgcgat aggcccagct gccgtccggg
      841 agacgcagcg tggtgtcttc gccgcagtcg ttgtgatcga aaaccaaata cgcatagccg
      901 cggcgaaaca gatcggcgtg gcgtgcggcg atgtcggcgg ttgatgcggc ggggctttgc
      961 gcggccgacg gtggcggcgg gttggtagag ttgccggaag aactttcttg ggcggggccg
     1021 actacgagaa gggcgttggt gcctttgccc tgagtggggc ccaacggaag ccggggcagt
     1081 ggcgttaccc cgaggggcag gccggcggga gcgatgatga cgggaacagg cgtgttcgaa
     1141 cgcggggtga aaagaccgac gcgaagc
//
LOCUS       DASPLT010001112         1045 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_259094, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001112 DASPLT010000000
VERSION     DASPLT010001112.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1045)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1045)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1045
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_259094"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..447)
                     /locus_tag="VEA63_11470"
     CDS             complement(<1..447)
                     /locus_tag="VEA63_11470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007362854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HYD84670.1"
                     /translation="MQQLHAYWRMEYIEAPRYPAKMKRPFSELPALGDDRAALIVHRT
                     RLAYLILNRFPYNPGHLLAVPFREVTELEQLTPAEAAALMAEVIFAKRLLTAALKPDG
                     VNVGFNLGSAAGGSIPHLHAHIVPRWNGDTNFMPVLGSTHVIPQALT"
     gene            659..>1045
                     /locus_tag="VEA63_11475"
     CDS             659..>1045
                     /locus_tag="VEA63_11475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HYD84671.1"
                     /translation="MNVASHSSPRRVVITGIGAVTPCGNSAPATWESLAAGRSGIRRL
                     TRFDATGCTAQIAGEVRDFDVTKPLAQPLRPRGPANEALTQVLTPKDVKKFGRFTHLG
                     AAASVEAYADSGLDAHRLEFSPERMGV"
BASE COUNT          180 a          271 c          395 g          199 t
ORIGIN      
        1 ggtcagggct tgtggaatga catgtgtgct ccccagaacc ggcatgaaat tggtgtcgcc
       61 gttccaacgc gggactatgt gagcgtggag gtggggaata ctgccgccgg cggccgagcc
      121 gagattgaag ccgacgttga cgccgtcggg cttgagggcg gcggtgagga gtcgtttcgc
      181 gaagatgacc tcggccatga gcgcggcggc ttcggccggc gtgagctgct cgagttcggt
      241 gacctcgcgg aagggcaccg cgagaaggtg gccgggattg taaggaaaac ggttcagaat
      301 gaggtaggcg agccgggtgc gatggacgat gagggcagcg cggtcatcgc cgagtgccgg
      361 caactcggaa aacgggcgct tcattttcgc cgggtagcgt ggcgcctcga tatattccat
      421 gcgccaataa gcgtgaagct gctgcatgtc ggaaagggag aagcgaaagc cctcggttgg
      481 gcaaagtcaa agcccgggtc acgcgcgagc cggcgggcgt ggagggctga ccgcaagcgt
      541 ttggctgagc cggggagttg aggcaacgag cagaaggttt gccgccagcg ggttgcggtt
      601 gatggaattt tatccttcgc acggtttaca gagcggactt tactcggccc ttttccgtat
      661 gaacgttgcg tctcattctt ctcctcgtcg ggtcgttatc accggtatcg gtgcggtcac
      721 accttgcggt aactccgctc ctgcgacttg ggagtcgctc gctgccgggc gcagcggaat
      781 ccggcggctc acgcgtttcg acgcgaccgg ttgcacggcg caaatcgcgg gggaggtgcg
      841 ggatttcgac gtgacgaagc cgctcgcgca gccgttgcgg ccgcggggtc cggcgaacga
      901 ggcgctgacg caggtgttga cgccgaagga cgtgaagaag ttcgggcggt tcacgcatct
      961 cggtgcggcg gcgtcggtcg aagcgtatgc ggattcgggc ttggacgcgc atcggctgga
     1021 gttttctccg gagcggatgg gcgtg
//
LOCUS       DASPLT010001113         2266 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_136612, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001113 DASPLT010000000
VERSION     DASPLT010001113.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 2266)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2266)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2266
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_136612"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            42..>2266
                     /locus_tag="VEA63_11480"
     CDS             42..>2266
                     /locus_tag="VEA63_11480"
                     /inference="COORDINATES: protein motif:HMM:NF037959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HYD84672.1"
                     /translation="MPPFALTIFLGAFLLFAVQPLLGKYILPWFGGSPGVWTTCMLFF
                     QTLLLGGYLYAHLSATRLAPRRQVWLHLALIAVAIALLPIIPGEHWRPRGDENPVSRI
                     LLLLGASVGLPYFVLSATGPLMQHWFSQAQPGRSPYRLYALSNAGSLLALLGYPFVFE
                     RAFSRQELSIYWSLGLAAFALACGWCAWRVWKSTPAFVSANATPTTAADDDPALAPTS
                     APRVPPLDRLLWFSLACIASILLLATTNKLSQDIAVVPFLWVLPLSLYLLTFILCFDH
                     PRWYSRGLFAGLFALGALIDIHLLFAGHNAPLPQQVIGYATTLFAGCMLCHGELFRLR
                     PAPARITSFYLHIAAGGAVGGFLVAVVAPLVFDRYLELHLGLWLLSYFVGVLAFRHRS
                     RSLAFGTGAGAFASVLVLPILDALAAKPDDLSLETLLQLQEFFREHWTFIVFLAVAFL
                     IGAIGRRGWIREWDLRVGNFLMLFSVGLGILLIAQVRHDSRDAVLTSRDFYGVLKIFE
                     HNQDQPEFRYKTLVHGVTSHGLQFSNDPMAAWPTTYYGEASGVGLVISQLPPQRPHHL
                     GLVGLGTGTVAIYGRGGDRVRIYEIDPAVERLARTEFTYLARSPAKVDVILGDARLSM
                     ERELERGESQQFDVLALDAFSSDAIPVHLLTKEAFAIYLRHLKPGGVIAVHTSNRYLD
                     LQPVVENLGRHLGLASVTVGDSPSDKEWWIFRSTWVLLSSNAAALESDEFHDSAEPPS
                     TR"
BASE COUNT          312 a          838 c          632 g          484 t
ORIGIN      
        1 cccgcgcgac cgttgcgtgt ctccgggcgc gacgcgcccg catgccgccc ttcgccctca
       61 ccattttcct gggagctttc ctgctcttcg ccgttcagcc gctcctcgga aaatatatcc
      121 tcccatggtt cggaggcagt cccggcgtgt ggacgacgtg catgctgttt tttcaaacgc
      181 tgctgctcgg cggctatctc tatgcgcatc tttccgccac gcgtctcgcg ccgcgccggc
      241 aagtgtggct gcacctcgct ctgatcgcgg tggcgatcgc tttgctcccg atcattcccg
      301 gcgagcactg gcggccacgc ggcgacgaaa atcccgtgtc gcgcattctc ctgctgctcg
      361 gagcgagcgt cggcctgccg tatttcgtgc tctccgccac cgggccgctg atgcaacatt
      421 ggttcagcca ggcgcaaccc ggccgctcgc cgtatcggct ctacgctctc tcgaacgccg
      481 gctcgctgct tgcgctgctc ggttacccct tcgtcttcga gcgcgcgttt tcacggcagg
      541 aactctcgat ctactggtcc ctcggactcg ccgcgttcgc actcgcctgc ggctggtgcg
      601 cctggcgcgt gtggaaatcc acgcccgctt tcgtcagcgc caacgcaact cccacgaccg
      661 ccgccgacga tgatcccgcg ctcgctccaa catccgcgcc gcgcgtgccg ccactcgacc
      721 ggctcctctg gttcagcctc gcgtgcatcg cttcgatcct gctgctcgcg accaccaaca
      781 aactcagcca ggacatcgcc gtcgtgccct ttctttgggt gctgccgctg agcctttatc
      841 tgctgacgtt cattctctgt ttcgatcatc cgcgctggta ttcgcgcgga ctctttgcag
      901 gcctcttcgc cctcggcgcg ctgatcgaca ttcacctgct cttcgccggc cacaacgctc
      961 cgctgcccca acaagtcatc ggctacgcca ccacgctctt cgccggttgc atgctgtgtc
     1021 acggtgaact ttttcgcctg cggccagcgc ccgcacgcat cacgtccttc tacctgcaca
     1081 tcgcggcggg cggcgccgtc ggcgggtttc tcgtcgccgt cgtcgcgccg ctggtgttcg
     1141 accgctatct cgaactgcac ctcggcctct ggctgctgag ctacttcgtc ggtgtgctcg
     1201 cgtttcgcca tcgcagccgc tcgctcgcct tcggcaccgg cgccggcgcc ttcgcttcgg
     1261 tgctcgtatt gccgatcctg gacgcgctgg ccgcgaagcc cgacgacctg tcgctggaaa
     1321 ctctcctcca actccaggaa tttttccgcg agcactggac gttcatcgtg ttcctcgccg
     1381 tggcctttct catcggtgcg atcggccgcc gcggctggat tcgcgaatgg gatctgcgcg
     1441 tcggcaattt cctcatgctg ttttccgtcg gactcggcat tctgctgatc gcgcaggtgc
     1501 ggcacgattc gcgcgacgcg gtgctcacca gccgcgactt ctacggcgtg ctgaaaattt
     1561 tcgaacacaa ccaggaccag cccgagttcc gctacaaaac cttggtgcac ggcgtgacgt
     1621 cgcacggcct tcagttttcc aacgatccca tggcagcgtg gcccacgacc tactacggcg
     1681 aagccagcgg cgtcgggctc gtgatctcgc aactcccgcc ccagcggccg catcacctcg
     1741 gtctcgttgg gctcggcacc ggcaccgtcg ccatctacgg tcgcggcggc gatcgcgtgc
     1801 gcatctacga aattgatccg gccgtcgagc ggctcgcgcg cacggaattc acctacctcg
     1861 cgcgctcacc cgccaaggtc gacgtcatcc tcggcgatgc ccgcctctcg atggaacgcg
     1921 agctcgagcg cggcgaatcg cagcagttcg acgtgctcgc gctggacgcg ttcagcagcg
     1981 acgccattcc agttcacctg ctgaccaagg aagcctttgc gatctatctg cgtcacctga
     2041 agcccggcgg cgtgatcgcc gttcacacgt cgaaccgcta tctcgatctt caacctgtcg
     2101 tcgaaaatct cgggcgtcac ctcggcctcg cctccgtcac cgtcggcgac agcccttccg
     2161 ataaggaatg gtggatcttt cgcagcacgt gggtgttgct ctcgagcaac gccgccgcgc
     2221 tcgaaagcga cgagttccac gactccgccg agccgccgtc gacgcg
//
LOCUS       DASPLT010001114         1363 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_259134, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001114 DASPLT010000000
VERSION     DASPLT010001114.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1363)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1363)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1363
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_259134"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..611)
                     /locus_tag="VEA63_11485"
     CDS             complement(<1..611)
                     /locus_tag="VEA63_11485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HYD84673.1"
                     /translation="MNAAVLPPPEPMDLAPLCAETALRLMQHGVESALIESLTRRVGI
                     ALGAERVEIALMANAVTVTVRIRGRSKTTVRRNEDRGINMHVAMEVQRLVLEAEAGKL
                     DRASYRERLLAIQPLHHRRWLVAVMVGLSCACFARLNHADWNACALVAVASAVAMVVR
                     QLLAHLHFSPLVVFAVTAFVATSVTAVGFVYELSQTPKAAMAA"
     gene            complement(693..>1363)
                     /locus_tag="VEA63_11490"
     CDS             complement(693..>1363)
                     /locus_tag="VEA63_11490"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HYD84674.1"
                     /translation="RRSVKLRASYGTAFRSPSFLDLYGRSAFYVGNPELEAERARGWD
                     AGVDYYLPENRGRWSATWFDTRFRDLIAYDFTVFPSTVRNIEQARTRGLEVSARHPIG
                     GTDLRLAYTYLEAINSTQQIRLVRRPRHALSADFWRELGRGFSAGVGVGFVADRRDVH
                     AETFATIDAEDYTVARIYAEWQASERLKLTLRLENLLDERYEEVNGYPALGRGVFAGA
                     AWRF"
BASE COUNT          277 a          498 c          369 g          219 t
ORIGIN      
        1 ctcgctgcca tcgccgcctt cggcgtctga cttagttcat aaacaaaacc caccgccgtc
       61 accgacgtcg ccacgaacgc ggtgaccgcg aaaaccacca aagggctgaa atgcagatgc
      121 gcgagcaact gccgcacgac catcgctacc gcactcgcga ccgccaccag ggcgcacgca
      181 ttccagtccg cgtggttcaa ccgcgcgaaa caggcacacg ataatccaac catcaccgcc
      241 accagccaac gtcggtgatg cagcggctga atcgccagca gacgttcacg atagctcgcg
      301 cggtcgagct tgcctgcctc ggcctccagc acgagccgct gcacttccat cgccacatgc
      361 atattgatgc cgcgatcttc gttgcgccgc acggtcgtct tgctccggcc ccgaatgcgc
      421 acggtgaccg tgacggcgtt cgccatcagc gcgatttcga ctcgctccgc accgagcgcg
      481 atcccgacgc gccgggtcaa cgactcgatg agcgcgctct ccacaccgtg ctgcatcaac
      541 cgcaacgccg tctccgcaca aagcggcgcc aggtccatcg gttccggagg cggcaaaacg
      601 gcagcgttca tcgtgatgtc ggcgtgaaaa cagaacgtgg aagaacaccg gtgcaagact
      661 cgccgcgcct tgcaccgttg cctggcccgt cctcaaaagc gccacgccgc cccggcaaac
      721 acgccgcgac caagggccgg gtagccattc acttcctcgt agcgctcatc gagaaggttt
      781 tccagacgca gagtcagctt caggcgctcg ctcgcctgcc attccgcata aatgcgagcg
      841 accgtgtaat cctccgcatc aatcgtcgcg aacgtctccg catggacgtc ccgccgatcg
      901 gcgacgaacc cgacgcccac gcccgcgctg aacccgcggc cgagttcgcg ccaaaaatcc
      961 gcactcagcg cgtgccgcgg tcgccgcacc agcctgatct gctgcgtcga attgatcgct
     1021 tcgagatacg tgtaagccag ccgcagatcg gtgccgccga tcgggtgtcg cgcagaaact
     1081 tccagccccc gcgtccgtgc ctgttcgata ttacgaaccg tgctcgggaa aaccgtgaaa
     1141 tcgtatgcga tcaaatcgcg aaacctcgta tcgaaccagg tcgcgctcca cctgccgcgg
     1201 ttttccggca aatagtaatc aacacccgca tcccagcccc gagcgcgttc cgcctcgagc
     1261 tccgggttgc ccacgtagaa cgcgctccgc ccgtaaagat cgaggaagct cggcgagcgg
     1321 aacgccgtgc cgtagctcgc ccgcaatttc acgctgcgac gtg
//
LOCUS       DASPLT010001115         1128 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_259171, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001115 DASPLT010000000
VERSION     DASPLT010001115.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1128)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1128)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1128
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_259171"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..691)
                     /locus_tag="VEA63_11495"
     CDS             complement(<1..691)
                     /locus_tag="VEA63_11495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HYD84675.1"
                     /translation="MTFRDWQNLSPASAARELHHRVDTALSPAQRRAVLASLPSEAAL
                     ADRFAAADRSAPLAGVPFFLKDLFDVVGEPTFAGSTFLPEVRPTPSRNSTLVDALARA
                     GAVLAGKTHLHEFAYGITGENPHYGDCEHPRFPGRTTGGSSSGSAAVVAAGIVPFAIG
                     TDTGGSVRVPAAFCGLYGFRLTPCDAFIADAFPLASTFDSAGWFTANADDFLTTISAL
                     IGNVDPPTRTPR"
     gene            911..>1128
                     /locus_tag="VEA63_11500"
     CDS             911..>1128
                     /locus_tag="VEA63_11500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009512202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HYD84676.1"
                     /translation="MNPEAKLAELGLALPNPPAAAGSYVPTVRTGNLLYCAGTISMAD
                     GKMTHVGQVGKEQTIESAKKSAEICALNT"
BASE COUNT          230 a          274 c          455 g          169 t
ORIGIN      
        1 cacgcggcgt gcgcgtgggc ggatcgacgt tgccgatgag cgcggagatc gtggtgagga
       61 aatcgtcggc gttggcggtg aaccagccgg cgctgtcgaa ggtcgacgcg agcggaaagg
      121 cgtcggcgat gaacgcatcg cacggcgtga ggcgaaaacc gtagagcccg cagaaagcgg
      181 cgggcacgcg aacggagccg ccggtgtcgg tgccgatcgc gaacgggacg atgccggcgg
      241 cgacaacggc ggcggaaccg ctgctcgaac cgccggtggt gcggccgggg aaacgcgggt
      301 gttcgcaatc gccgtagtgc ggattttcgc cggtgatgcc gtaagcgaat tcgtgcaggt
      361 gagttttgcc ggcgagcacg gcgccggcgc gggcgagagc gtcgacgagc gtgctgttgc
      421 gcgaaggcgt cgggcgaact tcgggaagaa aggtggagcc ggcgaaggtc ggttcgccga
      481 cgacgtcgaa aagatcttta aggaagaacg gcacgccggc gagcggggcc gagcggtcgg
      541 cggcggcgaa gcggtcggcg agggcggctt cgctgggcaa cgaggcgagg acggcgcggc
      601 gttgcgcggg tgagagcgcg gtgtcgacgc ggtggtggag ttcgcgtgcg gcggaggcgg
      661 gggagaggtt ttgccaatcg cggaaggtca cgggagagag cggggagacg gatgaaggat
      721 tcgaggcgaa atggtccgga ctaattcagt ttaaccacga atggacacga atggacacga
      781 atgaaaaggg ggagggggat tcgagtggaa agtgtcgggg cgtaaagccc ctcccacagg
      841 tgttgaagca gtttgttgac gcggaaaatc cgcgttgctt cggggaaacg tttcgcgagt
      901 ttagcggcgc atgaatcccg aagccaaact cgccgaactt ggacttgcct tgccgaatcc
      961 acccgcggcc gcgggcagct atgtgccgac ggtgcgaacg ggtaatctgc tttattgcgc
     1021 gggcacgatt tcgatggcgg atggcaagat gacgcatgtc ggacaagtcg ggaaagagca
     1081 gacgatcgag tcggcgaaga agtcggcgga gatttgcgcg ttgaacac
//
LOCUS       DASPLT010001116         1428 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_37199, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001116 DASPLT010000000
VERSION     DASPLT010001116.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1428)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1428)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1428
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_37199"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(215..1213)
                     /gene="galE"
                     /locus_tag="VEA63_11505"
     CDS             complement(215..1213)
                     /gene="galE"
                     /locus_tag="VEA63_11505"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009507482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HYD84677.1"
                     /translation="MNVLVVGGAGYIGSHCVRQLIAAGHRPVVLDNLVFGHRQAVAAD
                     LPLYTADLGDEAAVGKILEEEKIDVVMHFAAFAYVGESVQDPLKYYFNNVVATLHLLR
                     VMLAKGVKKFVFSSTCATYGVPDKTPITENLPQAPINPYGQTKLDIENALKSLAHATG
                     LSFAAFRYFNAAGAAEDGSIGEDHSPETHLIPLAIDAAIGRRGALQVFGGDYPTPDGT
                     CLRDYVHVDDLSRAHIAVFDKLAKPGTALFYNLGTGRPTSVLEVIRAVEKVTGKSVPH
                     TIAPRRAGDPPALYADSSKAKSELGWKVKFPDIESIVASTWQWHASHPDGYADRKK"
BASE COUNT          273 a          396 c          464 g          295 t
ORIGIN      
        1 cacgatttga agatcttcat ccaacacatg cggaggcagt gaagcagggg catccatgcg
       61 gctttcggac gccctgcttt gaaaatggtt tcctcgtttt cgctgggaaa attttgaacg
      121 cgagtgactc caacgcgttc cggattacgt aggtgaacgg atctcgggtt acgccagcgc
      181 gcgtttgcat ccgcgctgcg cgatttcagc ggtgttattt cttgcgatca gcgtatccgt
      241 ccggatggga cgcgtgccac tgccacgtgc tcgcgacgat cgattcgatg tcgggaaact
      301 tcactttcca gccgagctcg gacttcgctt tcgacgaatc ggcgtagagc gcgggcggat
      361 cgccggcgcg tcgcggcgcg atcgtgtgcg ggacgctttt gccggtgacc ttttccaccg
      421 cgcggatgac ctcgagcacg gaggttggcc ggccggtgcc gagattatag aagagcgcgg
      481 tgccgggttt cgcgagtttg tcgaaaacgg cgatgtgcgc gcggctgaga tcgtcgacgt
      541 gcacgtagtc gcgcaggcac gttccgtctg gcgtgggata gtccccgccg aaaacctgca
      601 gcgcgccgcg gcgtccgatc gccgcatcga tcgcgagcgg aatcaggtgc gtctcgggcg
      661 agtgatcctc gccgatcgag ccgtcctcgg cggcgccagc ggcgttgaaa tagcgaaacg
      721 ccgcgaagct gaggccggtg gcgtgagcga gagattttag agcattctcg atgtcgagtt
      781 tcgtctggcc gtagggattg atcggagctt ggggcagatt ctccgtgatc ggcgttttgt
      841 ccggcacgcc gtaggtcgcg caggtggagg agaacacgaa ctttttgacg cctttcgcga
      901 gcatcacgcg caacagatgc agcgtcgcga cgacgttgtt gaagtagtat ttcaacggat
      961 cctgcaccga ctcgccgacg taggcgaaag cggcgaagtg catcacgacg tcgatttttt
     1021 cctcttccag gattttgccc accgccgcct catcacccaa atccgccgtg tagagcggaa
     1081 gatcggcggc gaccgcctgg cggtgtccga agaccagatt gtccagcacc accgggcgat
     1141 ggcccgcggc gatgagctgg cgaacacagt gactgccgat gtagccggcg ccgcctacga
     1201 cgagaacgtt cataaaagag taagagccag atggattgcg gtgttgatgg agccgcgcaa
     1261 tgacaaatcc caacgggaga cgttcgcctc cgagggcagt tttcttgcga cgtgaagatt
     1321 cgacggtcga agcgcgccgt tttggaaaaa cgccggtgca tcacacctac gggaagattg
     1381 ctttcggcga ggcgtgaggt agcgattttc gtttcgccgc atcaatga
//
LOCUS       DASPLT010001117         1476 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_177992, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001117 DASPLT010000000
VERSION     DASPLT010001117.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1476)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1476)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1476
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_177992"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1053)
                     /gene="clpX"
                     /locus_tag="VEA63_11510"
     CDS             complement(<1..1053)
                     /gene="clpX"
                     /locus_tag="VEA63_11510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007360912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HYD84678.1"
                     /translation="MAKSSRMTLCSFCGKSQAEVKKIIAGPGVYICDSCVNVCKTIID
                     REVKTPAAEPRPPVRLIKPSEIKRTLDDFVIGQDHAKKVLSVAVYNHYKRLMFDSGQP
                     GKDSMALSPEFNDVDVEKSNILLVGPTGSGKTLLARTLAKILDVPFAISDATTLTEAG
                     YVGEDVENVVLRLLQNANYDVKRAECGIIYIDEIDKIGRKTENVSITRDVSGEGVQQA
                     LLKILEGTTCNVPPQGGRKHPNQEYVQINTANILFICGGAFVGLDSLVQRRLGQRSLG
                     FSSIRAQQDQGMKPEEMMKALAPEDLIRFGMIPEFIGRLPVVSVLDQLSVADLEKVLL
                     RTKNAMVKQYSKLFAMD"
     gene            complement(1114..>1476)
                     /locus_tag="VEA63_11515"
     CDS             complement(1114..>1476)
                     /locus_tag="VEA63_11515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HYD84679.1"
                     /translation="IVTYCVGMAASMSTVLLSAGTKGKRFALPNSRVMIHQPSGGAGG
                     QAADIAIAAREIIRWRKTLNEVIAKHTGKTPAQVEKDSDRDYYMSAQEAKDYGIVDHV
                     VESTREAAALGVPTTAAA"
BASE COUNT          275 a          471 c          426 g          304 t
ORIGIN      
        1 gtccatcgcg aagagcttcg aatactgctt caccatcgcg ttcttcgtgc gaagcaaaac
       61 cttctcgaga tcggccaccg aaagctgatc gagcaccgac accaccggca gacggccgat
      121 gaactccgga atcatgccga agcgaatgag atcttccggc gcgagcgcct tcatcatctc
      181 ttccggtttc ataccttgat cctgctgcgc gcgaatcgac gagaaaccga ggctgcgctg
      241 accgagccgg cgctgcacca aactatcgag ccccacaaac gcgccgccgc agatgaacag
      301 gatgttcgcc gtgttgatct gcacgtattc ctgattcgga tgcttgcggc cgccttgagg
      361 cggaacgttg cacgtcgtgc cttcgagaat cttcaacagc gcctgttgca cgccttcgcc
      421 cgaaacatcg cgcgtgatcg aaacgttctc cgtcttgcgg ccgatcttgt cgatttcgtc
      481 gatgtagatg atgccgcact ccgcgcgttt cacgtcgtag ttcgcgttct gcagcaaacg
      541 caggacgacg ttctcgacgt cttcgccgac gtagccggct tcggtcaacg tagtcgcatc
      601 cgaaatcgca aacggcacgt cgaggatctt cgccagcgtg cgcgcgagca gcgtcttgcc
      661 ggagcccgtc gggccgacca gcaggatgtt gctcttctcg acgtcgacgt cgttgaactc
      721 cggcgacagc gccatcgaat ccttcccggg ctggccggag tcaaacatca ggcgcttgta
      781 gtgattgtag accgcaaccg agagaacctt cttcgcgtga tcctggccga tgacgaagtc
      841 gtcgagcgtg cgcttgatct cggacggctt gatcaaccgc accggtggac gcggctcggc
      901 ggccggcgtt ttcacttcgc ggtcgatgat cgtcttgcag acgttgacgc acgagtcgca
      961 gatgtagacg cccgggccgg cgatgatctt cttcacttcc gcctgcgatt tgccgcagaa
     1021 ggaacaaagg gtcatgcgcg aggatttggc catggcgaaa agatgtagtt caactgcgct
     1081 gagtcgagga aggagacttt cttaacccta aattcacgcc gccgcagtcg tgggcacgcc
     1141 caacgccgcc gcttcgcgcg tcgactcgac cacgtgatcg acgatcccgt agtccttcgc
     1201 ttcctgcgcg ctcatgtagt aatcgcgatc ggaatccttc tcgacctgcg cgggagtctt
     1261 gcccgtgtgc ttcgcgatca cctcgttcaa cgtcttgcgc cagcgaatga tctcgcgcgc
     1321 ggcgatcgcg atgtcggccg cctggccgcc cgcgccgcca ctcggctggt gaatcatcac
     1381 gcgactgttc gggagcgcga accgcttgcc cttggtgccg gcggagagga gcaccgtgct
     1441 catgctcgcc gccatgccca cacagtaagt gacgat
//
LOCUS       DASPLT010001118         3680 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_136748, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001118 DASPLT010000000
VERSION     DASPLT010001118.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 3680)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3680)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3680
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_136748"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1052
                     /locus_tag="VEA63_11520"
     CDS             <1..1052
                     /locus_tag="VEA63_11520"
                     /inference="COORDINATES: protein motif:HMM:NF012381.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD84680.1"
                     /translation="PNPRFSMSTSVPFRAALSSVAPVDAPGHSAEDRFARVQSIRSSL
                     GSLARQIVPARDVPERSAPATIATGVRSLDARIGGVPRGALTEVCGPASSGRTTVLLS
                     LVAAMTRAGEVCALVDASDSFSPHSAADAGVDLRQLLWVRCNGKQPQKEKQQARFIAN
                     DFAGHDYVGHGSVEAKTVDPKGLASSANPFAARATSTAAARDRIERSQAFRRVEQALK
                     ITDLLLQGGGFGLIVIDLGDVTPEVARRVPMTSWFRFRRTVESTPTALVVLEQEPSAK
                     TCASLVMRAQRTEAQRTSPHCEAPTHARLLRGMEISIEIERASALIHDASFTRGAKKP
                     PRSARVDFNAEAKWC"
     gene            1084..3012
                     /locus_tag="VEA63_11525"
     CDS             1084..3012
                     /locus_tag="VEA63_11525"
                     /inference="COORDINATES: protein motif:HMM:NF013015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD84681.1"
                     /translation="MFAAIYIPDFSVEAVVRVRPELREQAVAVIEGVPPLAYVVAVND
                     RARELGLRTGMTRMQAEAFATSMDPRYARAAQANSEEPPTKDQKEDGDPRFKLVRDPI
                     HMHNAQENAWLPRQKRNAHTNPNVRVSLAQRSPAQEIAAHAALLDAAYAFSPRVEDTA
                     PDTVVLDVNGLERLFGAPPKIAREIARRASELGLEAQVAIAGNPDNAIHAARGFAGTT
                     VIPANHEAQRLGPLPLDVLFGAELNCRRVEWRKKKKPQEREFADRLTRMHETLDRWGI
                     RNFRALATLPPVSLSERLGAEGVRLQKLANGETTRELILTEPALEFEEALELEYPVEL
                     IEPLAFLLGRMLEQLCARLASRALSTNELRLRLQVEHWAGDEMTVEAAELRAAEKFID
                     RKLTLPVAMNDPRLFLKLMQLELNGNPPGAPVIKMWLATEPSRPRIAQAGLFMPLAPE
                     PEKLELTMARIHRVLGAAPSVAESSESVDVRAGSAELLDTHQPDAFRMVRFQPRTGEG
                     KPMNTEAATNNQRPTTALRVLRPAPAASVIVRDARPVRLTCNQIGAKENETHDNVVWA
                     AGPWRTSGNWWVFAEDSSGATASTEATPHAWHREQWDVALSVLKTTPDQRRERHVALY
                     RLSHDLLSQEWFVEGSYD"
     gene            3028..>3680
                     /locus_tag="VEA63_11530"
     CDS             3028..>3680
                     /locus_tag="VEA63_11530"
                     /inference="COORDINATES: protein motif:HMM:NF014826.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HYD84682.1"
                     /translation="MYTELHARSAFSFLEGASLPEELVEVCAQQGMSSMALLDRDGVY
                     GSARFHLAAKKIGLKALIGSDVSLPEGSLPLLVASRPGYQNLCRLVTQMKLRAPKNTP
                     SSTCPEELAQYSQGLICLTGGHDGPLAHSLDTGGMPAARRALEQLVTTFGRENVYVEL
                     QRHYDRAEEARNQAAIELARSLKLPLVAANGVHHARPEQRQIADVFTCIRNHRSLATA
                     "
BASE COUNT          708 a         1195 c         1143 g          634 t
ORIGIN      
        1 ttccgaaccc gaggttctcc atgagcacgt ccgtcccatt tcgcgccgca ttgtcttctg
       61 tcgctcccgt ggatgctccg ggccattcgg cagaggaccg gttcgcgcgg gtgcagtcca
      121 ttcgcagcag tctcggcagt cttgcccggc agatcgtgcc ggcgcgcgac gtccccgagc
      181 gctcggcgcc agcgacgatt gccactggcg tacgctcgct ggacgcgcgg atcggcggcg
      241 tgcctcgcgg agcactcacc gaagtgtgcg gcccggcgtc ttcgggacgc accactgtgc
      301 tgctttcgct ggtggctgcc atgacgaggg ctggggaagt ctgcgccctg gtggacgcga
      361 gcgacagctt ctctccgcat tcggccgccg acgcgggagt cgatctgcgg cagctgctct
      421 gggtgcgctg caacggaaag cagccgcaga aagagaagca gcaggcccga ttcatcgcca
      481 acgattttgc cggacatgac tacgtgggcc acggttccgt cgaggcaaag acggtggatc
      541 caaaggggct cgcgagttcc gccaatccgt ttgccgcacg ggcgacatcg acggcggccg
      601 cacgcgaccg catcgagcga agccaagcgt tccgccgtgt ggagcaggcg ctgaagatta
      661 ccgacctgct gctgcaaggc ggcggcttcg gcctgatcgt catcgatctc ggcgacgtta
      721 caccggaagt cgcacgccgc gtgccgatga catcgtggtt ccgcttccgc cgcacggtcg
      781 agagcacgcc taccgcgctc gttgtgctcg agcaggaacc ctcggccaag acatgcgcgt
      841 cgcttgtgat gcgcgcacaa cgcacggaag ctcagcgcac ctcaccacac tgcgaagcac
      901 cgacgcatgc caggctgttg cgcggcatgg aaatctctat agagattgaa cgcgccagcg
      961 cgctcatcca cgacgcatcg tttacgcggg gcgcaaagaa gccaccacgc agcgcgcggg
     1021 tggacttcaa cgcggaagcg aagtggtgct gaacgctgat ctctgaggac tcatttgctt
     1081 tgtatgtttg ccgccatcta catcccggat ttcagcgttg aggccgtcgt gcgagtgcgc
     1141 cccgagttgc gcgagcaggc ggtcgccgtt attgaaggcg taccgccgct ggcttatgtg
     1201 gtcgccgtga atgaccgtgc gcgcgaactg gggcttcgca cgggaatgac tcgcatgcag
     1261 gcggaagcgt ttgcaacgtc gatggatccg cggtacgcgc gggcggcaca ggcgaattca
     1321 gaagaacctc ctacgaagga tcagaaggag gacggcgacc cgcgattcaa actcgtgcgc
     1381 gatcccattc acatgcacaa cgcacaggag aatgcgtggc tgccccggca gaagcgaaac
     1441 gcgcacacga acccgaatgt gcgggtctca ctggcgcagc gttcgccagc gcaggagatt
     1501 gccgctcatg ccgcgctgct cgatgctgct tacgcctttt ccccgcgggt ggaagacacc
     1561 gcgccggaca cggtggtgct cgacgtgaac ggcctggagc ggctgttcgg cgcgcctccg
     1621 aagatcgcac gcgagatcgc gcggcgcgcc agcgaactcg ggctcgaagc acaagttgca
     1681 atcgccggga atccggacaa cgccatccat gccgcgcgcg gattcgccgg gaccacggtc
     1741 attcctgcca accatgaagc gcaacgcctg ggtccgcttc cgctggatgt gctcttcggc
     1801 gccgaactga attgccgtcg tgtcgagtgg aggaaaaaga agaagccaca ggaacgtgaa
     1861 ttcgccgacc gcctcacccg catgcacgag acgctggacc gctggggtat ccgcaacttc
     1921 cgcgcgcttg ccacgttacc gccggtgtca ctttccgaac gcctcggcgc ggagggcgtg
     1981 cgcctccaga aactcgcgaa tggcgagact acacgcgaac tcatcctcac cgaaccggcg
     2041 ctcgaattcg aggaagcgct cgaactcgaa tacccggtcg agttgatcga acccctggca
     2101 ttcctgctcg gacgcatgct tgaacaattg tgcgcgcgcc tggcttcccg cgcactttcg
     2161 acgaacgaac tgcgtctgcg tcttcaggtt gagcactggg ccggcgacga aatgacggtg
     2221 gaggccgccg aactgcgggc cgcggagaag ttcatcgatc gcaagctcac gttgccggtc
     2281 gcgatgaacg atccgcggct tttcctgaaa cttatgcaac tggaactcaa cggcaacccg
     2341 cccggcgcgc cagtaataaa gatgtggctt gcgactgagc catcgcgtcc gcgcattgcg
     2401 caagccggac tcttcatgcc actggctccc gagccagaga aacttgaact tacgatggcg
     2461 cgcatccatc gagtgctcgg cgcggcgccg tcggttgcgg agtccagcga gtccgtcgat
     2521 gttcgggccg gctccgcgga attgctcgat acgcaccaac ccgatgcgtt caggatggtc
     2581 cgatttcagc cgcggaccgg agaagggaag cctatgaaca ccgaagctgc aacgaacaat
     2641 caacgtccga ccacggcgct tcgcgtcctt cgtcctgcgc ctgccgccag cgtcattgtt
     2701 cgcgacgcgc gcccagtgcg gctcacatgc aatcaaatcg gcgcgaagga aaacgagaca
     2761 cacgacaacg tggtatgggc agccggcccg tggcgcactt ccgggaactg gtgggtcttt
     2821 gcagaggatt caagcggtgc gacggcatcg accgaggcga ctccgcatgc atggcatcgc
     2881 gaacagtggg acgtggcgct ttcggttttg aaaaccacgc ccgaccagcg tcgcgagcgt
     2941 cacgtcgcac tctaccggct ttcgcatgac ctgctcagcc aggaatggtt cgtggaaggc
     3001 agctacgact gatgactgaa taagcgaatg tataccgaac tccatgcccg gtccgcattc
     3061 agctttctcg agggcgcatc gctgcccgaa gaactggttg aagtgtgcgc gcagcaggga
     3121 atgagttcca tggctctgct cgaccgcgac ggcgtgtacg gatctgcacg cttccacctc
     3181 gctgcgaaga agattggcct caaggcactg atcggctccg acgtgagcct tcccgaggga
     3241 tcgctgcctc tgcttgtcgc gtcacgcccg gggtaccaga acctgtgccg gctcgttacg
     3301 cagatgaagc tgcgcgcgcc caaaaacaca ccgtcgtcca cctgtcccga agaactcgcg
     3361 cagtattcgc aaggactgat ctgccttacg ggcggacacg acgggccgct cgcgcactcg
     3421 ctcgacaccg gcgggatgcc cgccgcacgc cgcgcactgg agcaactcgt cacgacattc
     3481 ggacgcgaaa acgtgtacgt cgaactccag cgacactacg accgcgcgga ggaggcacgc
     3541 aaccaagccg cgatcgaact cgcgcgctca ctcaaattgc cgctcgttgc cgccaatggc
     3601 gtgcatcacg cccgtcccga gcagcgccag attgccgatg tctttacctg catccgcaat
     3661 catcgctcgc tcgccacggc
//
LOCUS       DASPLT010001119         2085 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_14120, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001119 DASPLT010000000
VERSION     DASPLT010001119.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 2085)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2085)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2085
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_14120"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1984
                     /locus_tag="VEA63_11535"
     CDS             <1..1984
                     /locus_tag="VEA63_11535"
                     /inference="COORDINATES: protein motif:HMM:NF015531.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HYD84683.1"
                     /translation="TEWTEYSIELPLNPAAREVFFGFLVAENGRGWVDDLQLLVDGRP
                     LRDAPKATRHDTAFDRDQEFTAGSRITLAELSPLQIQHLAVLCRVWGFLKYHHPKVTA
                     GDYHWDFELFRVLPKALSASNHAALSTTLLEWVQQIGPPQAIPEVPQSEADVHLPADH
                     RWLDHDATLSAPLRDTLKNIHAGRGRTASQVYVSFAPSVRNPAFNHEFDYRRIKLPDA
                     GYQLLGLFRYWNIIRYWFPYRDLIEENWDDVLAEFIPRIGLAHDVDAYALHFMALITK
                     VHDTHANLWSSLHLRPPVGDSQLPIAVRFVEGKMVVTHLLETAEEAPNPFMPGDLIRE
                     LNGVAIETLIENWKPFYAASNEPTRLRDIARSLTRGPAGPVNVRIERTHAMVDLTATH
                     IPTSSLKPASQTHDLPGPTFRLLTPEVAYLKLSSVRSADIPDYVSQARSTKGWIIDLR
                     NYPSEFVVFSLGSQLVGHPTDFARFTHGDLTAPGRFVFTSPIALSPHSPPLRPSALPA
                     SVINPRETTFADRPDFPNTSFKPDESTFQTGAAYTGKIVVLVDEVTQSQAEYTAMALR
                     AAGALIVGSTTAGADGNVSNIPLPGGLRTMISGIGVFYPDKRPTQRIGIVPDIEVTPT
                     PAGIRAGRDEILEAALRQVLGPDAPAAEIVRRAASR"
BASE COUNT          382 a          740 c          531 g          432 t
ORIGIN      
        1 caccgagtgg accgagtatt cgatcgaact cccgctcaat cctgctgcac gcgaagtgtt
       61 ctttggcttt cttgtcgctg aaaacggccg cggctgggtc gacgatcttc agcttctcgt
      121 cgatggacgg ccgctccgcg acgcgcctaa agctacgcgc cacgatacgg ccttcgaccg
      181 cgaccaggaa ttcaccgccg gctcccgaat cacgctcgcc gagttatccc cgctccaaat
      241 ccagcatctt gcggtcctct gccgcgtctg gggctttctg aaatatcatc atcccaaggt
      301 cacggcaggc gactaccact gggattttga actttttcgc gtgctgccga aggcattgtc
      361 cgcctccaac cacgcggccc tttccacgac tctgctcgaa tgggttcaac aaattggccc
      421 accccaggca atccccgagg tgccccagag cgaagcggac gtgcatctgc ccgcggatca
      481 ccgctggctc gaccatgacg cgacgctctc cgctccattg cgagacacgc tgaagaacat
      541 tcacgccggt cgtggccgga cggcctcgca agtttatgtt tccttcgcgc cgtcggtcag
      601 aaatcccgcg tttaatcacg agttcgacta tcgccgtatc aaacttccgg atgcgggcta
      661 ccagctcctc ggtctcttcc gctactggaa tatcattcgt tactggttcc cgtatcgcga
      721 cctgatcgag gaaaactggg acgatgttct cgcggagttc atcccgcgca tcggcctcgc
      781 ccatgatgtc gatgcctatg cactccattt catggcgctc attacgaagg ttcacgacac
      841 gcacgccaac ctctggagtt cacttcacct ccgcccaccg gtcggcgaca gccaattacc
      901 gatcgcagtc cgcttcgtcg aagggaagat ggtcgtgacc cacctcctcg aaaccgcgga
      961 agaggctccg aatccgttta tgccgggcga tctgatccga gaactgaacg gcgttgcgat
     1021 cgaaactctc atcgagaact ggaagccgtt ctacgccgcc tcgaacgaac cgacgcgtct
     1081 gcgcgacatc gcccgctcgc tcacccgcgg acccgcggga ccggtcaacg tgcgcatcga
     1141 gcgcacgcac gcgatggtcg atcttaccgc cacccacatt ccgacgtcgt ccctgaaacc
     1201 cgcctcccaa actcacgact tgcccgggcc gacgtttcgc ctgctcacgc ccgaggtcgc
     1261 ctacttgaaa ctctcctccg tcaggtccgc cgatattccc gactacgtca gccaggcccg
     1321 cagcacgaag ggctggatta ttgacctccg caactatccc tcggagttcg tcgtattctc
     1381 tcttggctcg cagctcgtcg gacacccgac cgactttgct cgattcacgc atggcgatct
     1441 caccgcccct ggtcgcttcg tgtttacatc gcctatcgcc ctctctccgc actcaccgcc
     1501 cctgcgtcct tcggctttgc cggcatcggt gatcaaccct cgcgaaacca ccttcgcgga
     1561 ccgtcctgat tttccgaata cgtctttcaa acccgacgaa tcgaccttcc agaccggcgc
     1621 tgcttacacc ggcaaaatcg tcgtcctcgt cgatgaagtc acccagagcc aggccgaata
     1681 caccgccatg gctttacgcg ccgccggcgc gttgatcgtc ggcagcacga ccgccggcgc
     1741 cgatggcaac gtttcgaaca tcccgttgcc tggcggactg cgcacaatga tcagcggtat
     1801 cggcgtcttc tatccggaca agcgccccac ccaacgaatt ggcatcgtgc ccgacatcga
     1861 agtgacgccg acacctgccg gcatccgcgc cggccgcgac gagatcctcg aagccgcact
     1921 gcgccaggtt ctgggtcccg atgctcccgc cgcagagatc gtccgccgcg cggcgtcgcg
     1981 ttgaaattgg ttacttcagt aacctcggag cggccaggct tgttgagccc gcgaattcag
     2041 ctagcggttt gtcatgcgca ccttttttac ccccttgctg tgcgt
//
LOCUS       DASPLT010001120         1080 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_14121, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001120 DASPLT010000000
VERSION     DASPLT010001120.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1080)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1080)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1080
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_14121"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1072)
                     /locus_tag="VEA63_11540"
     CDS             complement(<1..1072)
                     /locus_tag="VEA63_11540"
                     /inference="COORDINATES: protein motif:HMM:NF012722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MHYT domain-containing protein"
                     /protein_id="HYD84684.1"
                     /translation="MLALHVGVPVSYDPLLSMISMAAAALGAGLAFLIVNGRTVTGFH
                     VATGGVAMGLAIASMHYLGMASMRLPAMIRYDAVLFATSIGIAIAASSGALVLARHRV
                     ITGASQYWARSLSAVVMGAGIAGMHYVGMAAAQYIPSGATITGEGSMVGPWSLQSVLV
                     SAGLVIAVALLALAAKNAAERQVALESLEQKTAEAEVAARAKDVFLASLSHELRTPLN
                     PALLIASDGAANPEFSTAARQAFETVARSIQVEARIIDDLLDLTRVSRGLMQIESRVV
                     DVHVALQEAIAVVQPSLSSRRIDLQVNLSAVDKQIRGDAERLLQVFWNLLQNAVKFSS
                     EGGTIGVNTENEGPTVIVRVTDS"
BASE COUNT          213 a          372 c          305 g          190 t
ORIGIN      
        1 cgctgtctgt gacacggact atcaccgtcg ggccctcgtt ctcagtgttc accccgatcg
       61 tgccgccctc gctgctgaat ttcaccgcgt tttggagcag attccaaaac acctgcagca
      121 gccgctccgc atcgcctcgg atctgtttgt ccactgcgga caggttcacc tgcaggtcga
      181 tccttcgact gctcagcgac ggctgcacca ccgcgatcgc ttcctgcaac gcgacgtgca
      241 catcgaccac ccgcgattcg atttgcatca gcccccgcga gacgcgcgtg agatcgagca
      301 ggtcgtcgat aatccgggct tccacctgga tgcttcgcgc cacggtctca aaggcctgcc
      361 tcgctgcggt ggaaaattcc ggattcgccg cgccatcgct cgcgatgagc agcgccgggt
      421 ttagcggcgt gcgcaactca tgggacagcg aagccagaaa aacatccttt gctcgtgccg
      481 cgacttccgc ttcggcagtc ttctgctcga gcgattccaa cgcgacctgt ctttcggcgg
      541 cgttcttggc ggctaacgcg agcagggcaa cggcgatcac caatcccgcc gaaacgagca
      601 ccgattggag ggaccatgga ccgaccatcg atccttcacc cgtaatcgtt gcgccactcg
      661 gaatatactg cgcggcggcc ataccgacat agtgcatgcc agcaatcccg gcacccatta
      721 cgaccgcgct caacgacctc gcccaatact ggctcgcgcc cgtgatgacg cggtgccggg
      781 ccaacaccaa cgccccggaa gatgcggcga tcgctattcc gatggacgtc gcgaacagga
      841 cggcgtcgta gcgaatcatg gcgggcagtc gcatcgaggc catgccgagg tagtgcatcg
      901 aagcgatcgc cagtcccatg gcgactccac cggtcgcaac gtgaaagcct gtgaccgtgc
      961 gaccatttac aatgagaaac gccagccccg cgccgagcgc cgcggccgcc atggagatca
     1021 tgctcaggag cggatcgtaa ctcacgggca ccccgacgtg cagcgcgagc atgcccacaa
//
LOCUS       DASPLT010001121         1401 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_37249, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001121 DASPLT010000000
VERSION     DASPLT010001121.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1401)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1401)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1401
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_37249"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..451)
                     /locus_tag="VEA63_11545"
     CDS             complement(<1..451)
                     /locus_tag="VEA63_11545"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HYD84685.1"
                     /translation="MKHALRSLAKSPGFTVVAVLSLALGIGACTAVFSVANAVLLRAL
                     PVPNPHELRVLQWSGTDVRMSSYEGETLEAGNRVTAADCVHHPLFVALREQAADVAEV
                     FGFAPLQSTITRRHAEAVAVRGLMVSDNFFSGLGVRPLIGRTLAPGHD"
     gene            complement(693..>1401)
                     /locus_tag="VEA63_11550"
     CDS             complement(693..>1401)
                     /locus_tag="VEA63_11550"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HYD84686.1"
                     /translation="TLFAGENPIGRRIGNPDPANPAWLEIVGVVPDVERSVGAVPSAS
                     SFLILRPIAQEPWTYFTVALRSSNPSALAEPMRRIIASLDPDISPAQFGTIENVTRIV
                     TGVGKTLSTVLLCFALLGLFLASIGIYGVIARIVVQRTPEIGVRIALGAQLRDVVHMI
                     LFSGLRMTLLGTAIGLAGAFALGWVLKQIMPASGSSNPLLVVAVTLGLIAIGLLACWL
                     PARRAARIDPITALRAE"
BASE COUNT          287 a          356 c          548 g          210 t
ORIGIN      
        1 aatcgtgacc aggagcgagt gtgcgaccga tcagcgggcg cacgccgagt ccggaaaaga
       61 agttgtccga caccatcagg ccgcgcacgg cgacggcttc ggcgtgccgc cgggtgatcg
      121 tgctctgaag cggcgcgaag ccaaaaactt cggcgacgtc cgcggcctgc tcgcgcagcg
      181 caacaaacaa agggtgatgc acgcagtcgg cggcggtgac gcgattgccg gcctccagcg
      241 tttcgccctc gtaagacgac attcgcacgt cggtgccgct ccattggagc acgcgtaact
      301 cgtggggatt cggcaccggc aacgcgcgga gcagaaccgc attcgcgacc gagaatacgg
      361 cggtgcacgc accgatcccc agcgcgagcg agaggacggc caccacggtg aagcccggag
      421 atttcgcgag agagcggagg gcgtgtttca tggcgaggga ggagcgtcgg gacggagttt
      481 ttaaccgcta atctgcgcta atttttccga ggagggatgg cggatgaggg gttgcgtgct
      541 tcgcctgatc ggcgacgcgc gggcggtgga gagtgaagga gcgacgaggg ggggtggtcg
      601 ttctcgttct cttaatcgtt ctcgttctct cggcgtcggt gggatgggtt cggggcgaga
      661 acgagaaaga gaacgaagaa cgagaacgat gatcactcgg cgcggagggc cgtgatgggg
      721 tcgatgcggg cggcgcggcg ggcggggagc cagcaggcga ggaggccgat ggcgatgaga
      781 ccgagggtga cggcgacgac caggagcggg ttggaggagc cggaagcggg catgatttgt
      841 tttagcaccc aaccgagtgc gaaggcgccg gcgaggccga tcgcggtgcc gagcagggtc
      901 atgcgcaggc cggagaaaag aatcatgtgg acgacgtcgc ggagctgcgc gccgagcgcg
      961 atgcgcacac cgatttccgg cgtgcgttgg acgacaatgc gggcgatcac gccgtagatg
     1021 ccgatggaag cgaggaacag gccgaggagg gcgaagcaga gaaggacggt ggaaagcgtt
     1081 tttccgacgc cggtgacgat ccgcgtcaca ttttcgatgg tgccgaattg agcgggggaa
     1141 atatccgggt cgagggaagc gatgattcgg cgcatcggtt ccgcgagcgc gctgggattg
     1201 gacgagcgga gggcaacggt gaaataggtc caaggctcct gagcgatggg gcgaaggatg
     1261 agaaaggaac tggcgctcgg cacggctccg acggagcgtt cgacgtcggg aacgacaccg
     1321 acgatttcga gccaggcggg attggcggga tcgggattac cgatgcggcg accgatggga
     1381 ttttcgccgg cgaagagggt t
//
LOCUS       DASPLT010001122         1422 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_114662, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001122 DASPLT010000000
VERSION     DASPLT010001122.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1422)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1422)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1422
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_114662"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..744)
                     /locus_tag="VEA63_11555"
     CDS             complement(<1..744)
                     /locus_tag="VEA63_11555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HYD84687.1"
                     /translation="MKFVSLRVLAPLLVLLPMQISAATSASGAASSATATAVVRADQP
                     GPVINPNLYGHFAEHLGRCIYEGIWVGPDSSIPNTRGIRNDVVAALKKLNIPVLRWPG
                     GYFADEYHWKDGIGPREKRPSIYNSHWGGVVENNHFGTHEFMDLVEMLGCEAYISGNV
                     GSGTVQEMMEWVEYLTSDAQSPMANLRRANGRDKAWRVKYFGVGNESWGCGGSMTPEY
                     YANEYRRYNTFIKNYDRENLIYRIACGANA"
     gene            complement(1008..>1422)
                     /locus_tag="VEA63_11560"
     CDS             complement(1008..>1422)
                     /locus_tag="VEA63_11560"
                     /inference="COORDINATES: protein motif:HMM:NF018123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015153 - rhamnose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008645 - hexose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="L-rhamnose/proton symporter RhaT"
                     /protein_id="HYD84688.1"
                     /translation="KLVVVLIGGFTTNAVWCLALNFRNRTTHEYISAETPRGERVPRL
                     PNLLLCVLAGGTWYLQFFFYTMGETQMGRFAFSSWTLHMASIIIFSTLRGIALKEWRG
                     SSRRTHGYIAAGIAVLILSTLVVGYGNFLGARATH"
BASE COUNT          279 a          380 c          502 g          261 t
ORIGIN      
        1 ggcgttggcg ccgcaggcga tgcggtagat gaggttctcg cggtcgtagt ttttgatgaa
       61 cgtgttgtag cgcctgtatt cattcgcgta atactcgggg gtcatcgagc cgccgcagcc
      121 ccagctttcg ttgcccacgc cgaagtattt cacgcgccag gccttgtcgc ggccgttggc
      181 gcggcggagg ttggccatgg gagactgggc gtcggaggtg aggtattcga cccactccat
      241 catctcctgg acggtgccgc tgccgacgtt gccgctgatg taggcttcgc agccgagcat
      301 ctcgacgagg tccatgaact cgtgcgtgcc gaagtgattg ttttccacga cgccacccca
      361 gtgagaattg tagatggaag gacgtttttc gcgcggaccg ataccgtcct tccagtggta
      421 ttcgtcggcg aagtagccgc ctggccagcg aaggacgggg atgttgagct tcttcaaggc
      481 cgcgacgacg tcgttgcgga tgccgcgggt attggggatg gacgaatcgg ggccgaccca
      541 aatgccttcg tagatgcaac ggccgaggtg ttcggcgaag tgaccgtaga ggttggggtt
      601 gatcacgggg ccgggttgat cggcgcggac gacggcggtg gcggtcgcgg acgaggcggc
      661 gccggacgcc gaagtggcag cggagatttg catcggaagc aggacaagca gcggagccag
      721 gacgcggagg gagacgaatt tcataggacg aggggatgta acaacgacgc ggcgaatcgt
      781 cgctggtcgt tggggtgaga cgaagaaggt gaccgatcga tgcgcgcgga caaccggacg
      841 gtatcttgac cgtcaacgaa ccatgtccgc cgggtcgggg ggctgcgcag caacctcagc
      901 cggaagtgtg ccgttgaacc acgttacact cgcaaaaacg cagtgagaag gcggagcccg
      961 atctcgtcat cgggcttagg cttcaccttc aacggcacac tcggggctca gtgcgtggcg
     1021 cgggcgccga ggaagttgcc gtagccgacg acgagcgtgg agagaatcag caccgcgatg
     1081 ccggcggcga tgtagccgtg ggtgcggcga ctgctgccgc gccattcttt caaagcgatt
     1141 ccccgcagcg tgctgaaaat gatgatgctc gccatgtgga gtgtccacga actgaacgca
     1201 aaccggccca tttgcgtctc gcccatcgta taaaaaaaga actgcagata ccacgtgcct
     1261 ccggcgagga cgcagaggag caggttggga agccgcggca cgcgttcgcc gcgcggggtc
     1321 tcggcggaaa tgtattcgtg tgtggttcgg ttgcgaaaat tgagcgcgag gcaccagacg
     1381 gcgttggtcg tgaagccgcc gatcagaacc acgacaagtt tg
//
LOCUS       DASPLT010001123         1880 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_198505, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001123 DASPLT010000000
VERSION     DASPLT010001123.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1880)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1880)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1880
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_198505"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..717
                     /gene="eutB"
                     /locus_tag="VEA63_11565"
     CDS             <1..717
                     /gene="eutB"
                     /locus_tag="VEA63_11565"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007362485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008851 - ethanolamine ammonia-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit EutB"
                     /protein_id="HYD84689.1"
                     /translation="QSCVLAHVTTQLEAIRRGAPVDLVFQSVAGTEAANKSFGITLAL
                     LAEAHDAARSLHRGTAGENVMYFETGQGSALSADAHHGVDQQTCEARAYAVARHFQPL
                     LVNTVVGFIGPEYLYDGKQIIRAGLEDHFCGKLLGLPMGVDVCYTNHAEADQDDMDTL
                     LTLLGVAGCNYIMGVPGADDIMLGYQSTSYHDSHYLRQALGLRPAPEFEAWLDRMHLV
                     RDGHALLPVPPAHPLLAASP"
     gene            714..1523
                     /gene="eutC"
                     /locus_tag="VEA63_11570"
     CDS             714..1523
                     /gene="eutC"
                     /locus_tag="VEA63_11570"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: protein motif:HMM:NF003971.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008851 - ethanolamine ammonia-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit EutC"
                     /protein_id="HYD84690.1"
                     /translation="MNAPPPAVSPLPRPPSFSPVDLWAPLRRFTSARIALGRAGGSFR
                     TETLLDFRLAHARARDAVTMPFNADALATDLTRADIDTFRVATRVSDRAEFLLRPDLG
                     RQLSAESRHALEAQRAAWGERDLAIIVSDGLSALAAARQIEPTLAALLPQLRAAGFWL
                     YPVFIAPFARVKLQDEIGELLGARQTLMLLGERPGLAAPDSLGAYFTFAPCAQRTDAD
                     RNCVSNIRPEGLSPANAAQTLACLLSESARRRISGIALKASASEVPAELTP"
     gene            complement(1577..1653)
                     /locus_tag="VEA63_11575"
     tRNA            complement(1577..1653)
                     /locus_tag="VEA63_11575"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1617..1619),aa:Val,seq:gac)
BASE COUNT          324 a          701 c          519 g          336 t
ORIGIN      
        1 caatcctgcg tgctcgcgca cgtcaccacg cagctcgagg cgattcgacg cggagcgccg
       61 gtcgacttgg tgtttcaatc cgtcgccggc accgaagccg ccaacaaaag cttcggcatc
      121 acgctcgccc tgctcgccga agctcacgac gccgcgcgct cgctccatcg cggcaccgcc
      181 ggcgagaacg tgatgtattt cgaaaccggc cagggctccg ccctctccgc cgatgcccac
      241 cacggcgtcg atcagcaaac ctgcgaagcg cgcgcctacg ccgtcgcgcg gcattttcag
      301 ccgctcctcg ttaacaccgt cgtcggtttc atcggccccg agtatctcta cgacggcaaa
      361 caaatcatcc gcgccggtct cgaagatcac ttctgcggaa aactgctcgg actgccgatg
      421 ggcgtcgacg tctgctacac caaccacgcc gaagccgatc aggacgacat ggacacgctg
      481 ctcacgctcc tcggcgtcgc gggctgcaac tacatcatgg gcgtgcccgg cgccgacgac
      541 atcatgctcg gctaccaatc gacatcgtat cacgacagcc actacctccg ccaggcgctc
      601 gggcttcgac cggcgcccga gttcgaggcg tggctcgatc ggatgcacct cgtccgcgat
      661 ggccacgccc tgcttcccgt tccacccgct catcctttgc ttgccgcttc gccgtgaacg
      721 caccaccgcc tgccgtctcc ccgctgcctc gcccgccgtc cttctcgccg gtcgatcttt
      781 gggcaccgct tcggcggttc acttccgccc gcatcgcgct cggccgcgcc ggaggaagtt
      841 tccgcacgga aacccttctc gattttcgcc tcgcccacgc ccgcgctcgc gacgcggtca
      901 cgatgccgtt caacgccgac gcgctcgcca ccgacctgac gcgagccgac atcgacacgt
      961 ttcgcgtcgc aacgcgcgtt tccgatcgcg cggagttttt gctccgcccg gatcttggcc
     1021 gacaactctc cgccgaatcc cgccacgccc tcgaagctca gcgcgcggct tggggtgagc
     1081 gcgatctcgc gatcatcgtc tccgacggcc tttcggcgct cgccgcggcg cgccaaatcg
     1141 aaccgacgct cgccgcacta ctgccgcaat tacgcgccgc aggtttttgg ctctatccgg
     1201 ttttcatcgc gcccttcgcg cgggttaaac tgcaggacga aatcggcgag ctgctcggcg
     1261 cgcgccaaac gctgatgctt ttgggcgaac gccccggcct cgccgcgccc gatagcttgg
     1321 gagcgtattt cacgttcgca ccgtgcgcgc aacgcaccga tgccgatcgc aactgtgttt
     1381 ccaacatccg cccggagggc ctttcgcccg cgaacgccgc ccaaaccctc gcgtgcctcc
     1441 tgtccgaatc ggcccgccgc cgcatcagcg gcatcgctct caaagccagc gcatccgaag
     1501 tccccgccga gctcacacct tgagccggcg acgggcacaa aaaaaccgcc gagcttgtcg
     1561 gcggaagtcc ttcaaatggt ggaccttact ggactcgaac cagtgacctt ttcgatgtca
     1621 acgaaacgct ctaaccaact gagctaaagg tccgctttga agagccggag agtaagcagg
     1681 agtcgcgcat gcttcggcaa gaaaatttcc aagagaaaat tgcgccgccg taagtaagtc
     1741 tcacgcagcc gaattggtgc cgcgttcgcg cgttgcagcc gcgcctgaga tcgtcccgcg
     1801 gcgagcgatt cctctcacgt tccaacgctg cgagcggttg caccagagct gaccaccagg
     1861 tggtgcatgg cgcccgctcg
//
LOCUS       DASPLT010001124         1208 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_279096, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001124 DASPLT010000000
VERSION     DASPLT010001124.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1208)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1208)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1208
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_279096"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            84..1076
                     /gene="galE"
                     /locus_tag="VEA63_11580"
     CDS             84..1076
                     /gene="galE"
                     /locus_tag="VEA63_11580"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HYD84691.1"
                     /translation="MNVLVVGGAGYIGSHCVRQLVAAGHRPVVLDNLVYGHRKAVAPE
                     IPLHEVNLGDEAAVGRILREEKIEVVMHFAAYCYVGESVTDPLKYYFNNVAATLHLLN
                     AMLAANVKKFVFSSTCATFGVPATLPIHENLPQAPINPYGQTKLDVENLLKSLAHANG
                     LSFAAFRYFNAAGAAEDGVIGEDHDPETHLIPVAIDVALGKRPHLQLFGTDYPTPDGT
                     CLRDYVHVDDLSRAHIAVFDKLSTPGAQLFYNLGTGKPSSNREVICAVEKILGKPVKV
                     IESPRRPGDPPALYADSSKAQRELGWKIKFPDIEAIVATAWKWHSSHPQGYADR"
BASE COUNT          229 a          431 c          319 g          229 t
ORIGIN      
        1 gcgaacgccc gccctccaga tttgtcattg ccgggcgccg ttcgcgcggc gaatcctctc
       61 tgctcactcg ctactctttt accatgaacg ttctcgtcgt tggaggcgcc ggttacatcg
      121 gcagtcattg tgttcgccaa ctcgtcgccg ccggccaccg ccccgtcgtc ctcgacaatc
      181 tcgtctacgg ccatcgcaag gctgtcgcgc ccgaaattcc gctgcacgag gtcaatctcg
      241 gcgacgaagc cgcggtcgga cgcatccttc gcgaggagaa aattgaagtc gtgatgcact
      301 tcgccgccta ttgctacgtc ggcgaatccg tcaccgatcc gctgaaatac tatttcaaca
      361 atgtcgccgc caccttgcat ctgctcaacg cgatgctcgc ggcgaatgtg aagaagttcg
      421 tgttctcctc gacctgcgcg accttcggcg tgccggccac gctgccgatt cacgaaaacc
      481 ttccgcaggc cccgatcaat ccctacggcc aaacgaagct cgacgtcgaa aacctcctca
      541 aatctctcgc gcacgcgaac ggcctcagct tcgccgcgtt tcgctacttc aacgccgccg
      601 gcgccgccga ggacggcgtc atcggcgagg atcacgatcc cgagacgcat ctgatccccg
      661 tcgcgatcga tgtcgcgctc ggcaagcgtc cgcatcttca gcttttcggc accgattatc
      721 ccacgcccga cggcacgtgt ctccgcgatt acgttcacgt cgacgatctc agccgcgcac
      781 acatcgcggt cttcgacaag ctctccacgc ccggcgcgca gctcttctac aatctcggca
      841 ccggcaaacc gtcgtcgaat cgcgaagtga tttgcgccgt cgaaaaaatc ctcggcaaac
      901 cggtaaaggt catcgagagc ccgcgtcgcc ccggcgatcc gccggcgttg tatgccgact
      961 cgtcgaaagc ccagcgcgag ctcggctgga aaataaagtt tcccgacatc gaagcgatcg
     1021 tcgccaccgc gtggaagtgg cattcgtccc atccccaagg ctacgccgac cgataggtct
     1081 accgctttat caatcgctcc gacgtagcgg cagacctccg cgcctgcctg cttgcctcgg
     1141 gtgcgatcgc gagagaaccg cgacacacgg accgcagagc aggcaggcac ggaggcctgc
     1201 cgctacct
//
LOCUS       DASPLT010001125         1357 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_14148, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001125 DASPLT010000000
VERSION     DASPLT010001125.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1357)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1357)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1357
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_14148"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1357
                     /locus_tag="VEA63_11585"
     CDS             <1..>1357
                     /locus_tag="VEA63_11585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HYD84692.1"
                     /translation="LWATHTQGVVTFTPRGESYEIDSSTFDLINAQFPAVHLLPDNDV
                     WFSAGRALYHVERQHGGEARQAGQPMLVSVIDGQTNAELFAHSNAAVPLRLPFAQNNL
                     SFRFFSGSYAWRRSPVYEFRLNGTDEWSPLGSGSFLSFLELREGRYRMEVRMVENKKP
                     IGSPASFNFEILAPWHRTWPAYGLYGLGVAAALFGIVRWSGHRAYHRNLALEKLVDER
                     TRQLKATMEKLNAETRNAATLAERDRLAGEIHDSLQQGLSGLILQLDATLKLPSAAGD
                     LRSRLEVARNMVSFTRHEVQHAVWDMESPLLEDTELSEALRKLTDLISPGTAIIDIAV
                     SGSPVALPSAIKHHLLRIAQEAITNAVRHASPRRISVALGYQPQSISLAVSDDGVGFR
                     PDDVLSKSFGHFGLRGLRSRATKLGGTLQIQSEPGRGASIRVVVPFTPAPLSSSDASD
                     YS"
BASE COUNT          250 a          471 c          377 g          259 t
ORIGIN      
        1 gctctgggcc acgcacactc aaggcgtcgt gaccttcacg ccgcgcggcg agagctacga
       61 gatcgatagc tccacgttcg atctcatcaa tgcgcagttt cccgccgttc acctcctgcc
      121 cgacaacgac gtttggttct ccgccggacg cgcgctctat catgtggagc ggcagcacgg
      181 cggcgaagcc agacaggcgg gccagcccat gcttgtatcc gttatagacg gacagacgaa
      241 cgccgaactg tttgcccatt cgaacgccgc cgtcccgctc cgcctcccgt ttgctcagaa
      301 caacctcagt ttccgtttct tctcgggaag ttacgcgtgg cgtcgctcgc cagtttatga
      361 atttcggctg aacggcaccg acgaatggtc gcccctcggc tccggttcgt tcctgagttt
      421 cttggaactg cgcgaaggcc gctaccgcat ggaagtcagg atggttgaaa acaaaaagcc
      481 catcggctct ccggcttcgt tcaatttcga gatcctcgcc ccatggcacc gcacctggcc
      541 ggcgtatggc ttgtatggct tgggcgtcgc cgccgcgttg ttcggcatcg tgcgatggtc
      601 gggccatcgt gcgtatcatc gcaatctcgc gctggaaaaa ctcgtcgacg aacggacgcg
      661 ccagttgaag gcgacgatgg aaaagctaaa cgccgaaacg cgcaacgccg ccaccctcgc
      721 ggagcgcgac cggcttgccg gcgagattca cgacagcctg caacagggcc tcagcggact
      781 catccttcaa ctcgacgcca cgttgaaact tccttcggcc gccggcgacc tccgctcgcg
      841 gctcgaagtc gcgcgcaaca tggtgtcgtt cacccgacat gaagtgcaac acgccgtgtg
      901 ggacatggag tcgcccctgc tcgaagacac cgaactgagc gaagctctcc gcaaactcac
      961 cgatctcatc agccccggca ccgcgatcat cgacatcgcc gtgtcgggtt cacccgtcgc
     1021 gctcccgtcg gcgatcaagc atcatctact ccgaatcgcc caggaagcga tcaccaacgc
     1081 cgtgcgacac gcttccccgc ggcgaatctc cgtcgcgctc ggttatcagc cgcaaagcat
     1141 ttctctcgcg gtgagcgacg atggagtcgg cttccgtcct gacgacgtcc tctcgaaaag
     1201 cttcggccac ttcgggcttc gcggcctccg gtctcgcgcc acgaagctcg gcggcacgct
     1261 gcagattcaa agcgagcccg gccggggcgc gtcgattcgc gtcgtcgttc cgttcacgcc
     1321 agcaccgctc tcctcttccg atgcaagcga ctactca
//
LOCUS       DASPLT010001126         1103 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_178066, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001126 DASPLT010000000
VERSION     DASPLT010001126.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1103)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1103)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1103
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_178066"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1103
                     /locus_tag="VEA63_11590"
     CDS             <1..>1103
                     /locus_tag="VEA63_11590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007363965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HYD84693.1"
                     /translation="DVVLQYNDSYNDQVLCYTNTIHNPDGGTHLSGFRSALTRAINQH
                     AKANNLLKDKDPQITGDDVREGLAAVISIKHSDPKFESQTKVKLLSPEVESIVGSVSY
                     EGLMFTFSANPNIAKRIVDKALNAARAREAARKARETVRKGALTSGGLPGKLADCSER
                     DPSLCELYIVEGDSAGGSAKQGRDRRYQAILPLRGKLINTEKARIDKVLANEEIRTLI
                     TACGTGIGDHEGEGAFDANKARYHKIIIMTDADVDGSHIRTLLLTFLYRQMRGLLDRG
                     FVYIAQPPLYKIKRKKREQYVDNDEQLNRILLELGSEDVVLTRLKDQHVFAPAQIDKI
                     VEALAAIEKLGSGVTRYGASLNEYLDQHDPATH"
BASE COUNT          249 a          412 c          278 g          164 t
ORIGIN      
        1 tgatgtcgtc ctccaatata acgactccta caacgaccag gtcctctgct acaccaacac
       61 gattcacaac cccgacggcg gcacgcatct ctccggtttc cgcagcgcgc tcacccgcgc
      121 gatcaatcag cacgcgaagg ccaacaatct gctcaaggac aaagacccgc agatcaccgg
      181 tgacgacgtc cgcgaaggtc tcgccgccgt catctccatc aagcacagcg accccaagtt
      241 cgagtcgcag accaaggtca aactcctctc gcccgaggtc gaaagtattg tcggctccgt
      301 ttcctacgag ggcctgatgt tcaccttcag cgccaacccg aacatcgcga agcgcatcgt
      361 cgacaaagcc ctcaacgccg cccgcgcccg cgaagccgcc cgcaaggccc gcgaaaccgt
      421 tcgcaaaggc gcgctcacca gcggcggcct ccccggcaaa ctcgccgact gctccgaacg
      481 cgatccttcg ctttgcgaac tctacatcgt cgagggcgac tccgccggcg gctccgcgaa
      541 acaaggccgc gaccgccgtt atcaggcgat ccttccgctg cgcggcaaac tcatcaacac
      601 ggagaaagcg cgtatcgaca aggtcctcgc caacgaggaa atccgcacgc tcatcaccgc
      661 ctgcggcacc ggcatcggcg accacgaggg cgagggcgcg ttcgacgcca ataaggcgcg
      721 ctatcacaag atcatcatca tgacggatgc ggacgtcgac ggctcgcaca tccgcacgct
      781 gctcctgacg tttctctacc gccaaatgcg cggcctcctc gaccgcggct ttgtctacat
      841 cgcccagccg ccgctctaca aaatcaagcg caagaaacgc gagcaatacg tcgacaacga
      901 cgaacagctc aaccgcatcc tcctcgagct cggctccgag gacgtcgtgc tcacgcgtct
      961 caaggaccag cacgtcttcg cgccggcgca aatcgacaaa atcgtcgaag ccctcgccgc
     1021 catcgagaaa ctcggcagcg gcgtcacgcg ttacggcgcc tcgctcaacg aatacctcga
     1081 tcagcacgat ccggcgacgc acg
//
LOCUS       DASPLT010001127         1013 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_37287, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001127 DASPLT010000000
VERSION     DASPLT010001127.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1013)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1013)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1013
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_37287"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..818)
                     /locus_tag="VEA63_11595"
     CDS             complement(<1..818)
                     /locus_tag="VEA63_11595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="immunoglobulin domain-containing protein"
                     /protein_id="HYD84694.1"
                     /translation="MTVRASQAGSSNYNSASNVDRSFLVTIGSAVVTTPEQVPAPPSG
                     GSATLEVTNSNPNVSYQWQRNGANLEGATGASLTLGDVQPPTAGLYTYTATVPGGAGG
                     TSEPVIVGLSTDEKVVGTGEEVGADIVHPNGNTYDQVLLEGNGASITSNPNQITRISF
                     VDLSNDIVQVEFSGVGTLSVVMDGASTPTPAANYNQPGVDYVKGHVGLVITGANETSN
                     LSIISVGPITAVNQSLFKSDVTYDGVANIAFVAIQSSNGKFGSIRTGNVHYFAS"
     gene            832..>1013
                     /locus_tag="VEA63_11600"
     CDS             832..>1013
                     /locus_tag="VEA63_11600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD84695.1"
                     /translation="MAVNVPPEIEAGPLITENDTGKPDVAVAESDTVSPMINVLIEAK
                     AIDCDAGWIVSVPPTYV"
BASE COUNT          179 a          320 c          308 g          206 t
ORIGIN      
        1 tcgctcgcga agtagtgcac gttacccgtg cgaatactgc cgaacttgcc gttgctgctc
       61 tggatcgcga cgaacgcgat gttcgccacg ccgtcgtaag tgacgtcgct cttgaacagg
      121 ctctggttca ccgccgtgat cggaccgacg ctgatgatcg agagattgct cgtctcgttc
      181 gcgcccgtga tcacgaggcc gacatggccc ttcacgtagt cgactccggg ttgattgtag
      241 ttggccgcag gcgtcggggt cgaagcgccg tccatcacca ccgacaatgt gccaacacca
      301 ctgaactcga cctgcacaat gtcattcgac aaatccacga acgagatgcg cgtgatctga
      361 ttgggattcg acgtgatcga tgcgccgttc ccttcgagca gcacctggtc gtaagtgttg
      421 ccgttcgggt gaacgatgtc ggcgccaact tcttcgccgg tgccgacgac tttctcgtcc
      481 gtcgaaagcc ccacgatcac cggttcgctc gtgccgccgg ctccgcccgg cacagtcgcc
      541 gtgtaagtgt aaagaccggc cgtcggaggc tgcacatcgc caagcgtgag actcgcgccg
      601 gtggcgcctt ccaaattcgc gccgttgcgc tgccattggt agctcacgtt cggattgctg
      661 ttcgtcactt cgagggtggc gctgccgccg ctcggcggcg cgggcacctg ctccggagtc
      721 gtcaccacgg ccgagccgat cgtaacgagg aaactgcggt ccacgttcga ggcgctgttg
      781 taattcgagc tgcccgcttg cgaagcgcgc acggtcactt cgccgacgcc cgtggcggtt
      841 aacgttccgc ccgaaatcga agctggtccg ctgatcaccg aaaacgacac cggcaagccc
      901 gatgtggctg tggccgagag cgacacggtt tcgccgatga tcaacgtgtt gatcgaggca
      961 aaggcgatcg actgcgatgc cggctggatc gtgagcgttc ctccgacata cgt
//
LOCUS       DASPLT010001128         1138 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_37288, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001128 DASPLT010000000
VERSION     DASPLT010001128.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1138)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1138)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1138
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_37288"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(392..>1138)
                     /locus_tag="VEA63_11605"
     CDS             complement(392..>1138)
                     /locus_tag="VEA63_11605"
                     /inference="COORDINATES: protein motif:HMM:NF014305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HYD84696.1"
                     /translation="DRRIAASLLLTAMLLSGCQTPTRAPDARQSPERITVTVKQEYQY
                     LRYAPADYGADQSKRWPLVLFLHGAGERGHDLELVKKHGLPKRVEQGAEFPFVMIAPQ
                     CPPGEWWNVFALEGLIEEVAHAERIDRDRIYLTGLSMGGFGTWALALRHPERYAAILP
                     VCGGGERQLARRLREMPIWAFHGDADQVVPVERSQEMIDAIKKLGGSPRFTAYPGVGH
                     DSWTATYDDPEVYEWLLAQRRGGKLTADLR"
BASE COUNT          218 a          420 c          301 g          199 t
ORIGIN      
        1 cgcccgtcac ctcgctttac cctgaatgcc gtagcggcag gcctccgtgc ctgccagctc
       61 tagctctcgc aagcagccag cgccggacgc cttccgtgac caccgaaagg caatgtgaac
      121 caaccggcgg tgctgagcag acggctgcgt tgatccggaa tccggacgtt gattgaaact
      181 catccttacg atgccgcaga cgatgagcgt cgaccagaat cggctgcgcc caacgcatga
      241 cacgccgccc ttcctccggc tctccacaat gtcatataat gtatgacatt cgccgtccgc
      301 gcattgatcc gccctgacgt caggccggac gccctcgcct cgccgattag gcgaggcgcg
      361 cactctcgcg cttgcctaac gcctccgaaa attagcgaag atcagcggtt aacttccccc
      421 cgcgcctctg cgccagcaac cactcgtaaa cctccggatc gtcataggtc gccgtccacg
      481 agtcgtgccc aactcctgga tacgcggtga agcgcggcga tccgccgagc tttttgatcg
      541 catcgatcat ctcctgcgag cgttcaaccg gcaccacctg atccgcgtcg ccatgaaacg
      601 cccagatcgg catctcgcgc aaccggcgcg caagttgccg ctcacccccg ccgcacaccg
      661 gcagaatcgc ggcgtaacgc tccggatgcc gcaacgccag cgcccacgtg ccaaacccgc
      721 ccatgctcag gcccgtgaga taaatccggt cgcgatcgat ccgctccgca tgcgccactt
      781 cctcgatcaa tccttccagc gcgaaaacat tccaccactc gccgggcgga cactggggcg
      841 cgatcatcac aaacggaaat tccgccccct gctccacccg cttcggcaat ccgtgcttct
      901 tgaccagctc gagatcgtgg ccgcgctcgc ccgcaccatg aaggaagagc accagcggcc
      961 agcgtttgct ctggtccgcg ccgtagtcgg cgggcgcgta acgaagatac tgatactcct
     1021 gtttcaccgt caccgttatc cgctccggcg actgccgtgc atccggagcg cgcgtgggtg
     1081 tctggcatcc ggacaacagc atcgccgtaa gtaacaagct ggcggcgatc cgccggtc
//
LOCUS       DASPLT010001129         1159 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_73632, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001129 DASPLT010000000
VERSION     DASPLT010001129.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1159)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1159)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1159
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_73632"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..257)
                     /locus_tag="VEA63_11610"
     CDS             complement(<1..257)
                     /locus_tag="VEA63_11610"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007359112.1"
                     /note="catalyzes the formation of tyrosyl-tRNA(Tyr) from
                     tyrosine and tRNA(Tyr); Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HYD84697.1"
                     /translation="MTILDDLQWRGLYADCTDLDALRQRLAQGPVTLYCGFDPTGDSL
                     HVGHLMGQLTLRRFQLAGHHPVALAGGATGMVGDPSGKSAER"
     gene            474..947
                     /locus_tag="VEA63_11615"
     CDS             474..947
                     /locus_tag="VEA63_11615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HYD84698.1"
                     /translation="MPLAIGSKAPDFTLKTKTADGLKDIKLSDNFGQQQTVLLFFPLA
                     FTGVCTQEMCDQSAGLGDYEKLGAKVIAISVDSPFAQEAWAKANKISLTIVSDLNKEV
                     TKAYDVVFPMLAGVGDTAARAAFVIGKDGVIKYAEQTPTPKDLPNFAAVKAALAK"
BASE COUNT          228 a          339 c          381 g          211 t
ORIGIN      
        1 cgttccgcgg acttgcccga cggatccccg accatgccgg tggcgccgcc ggcgagagcg
       61 acggggtgat ggccggcgag ttgaaaacgg cgcaacgtga gctggcccat caaatgcccg
      121 acgtgcagcg aatcgcccgt cggatcgaag ccgcaataga gcgtcaccgg gccttgggcg
      181 aggcgctggc ggagcgcgtc gaggtcggtg cagtcggcat aaaggccgcg ccattgcagg
      241 tcgtcgagaa tggtcatggg agtggagcag ttaacggggc gggcgaggag cggcgcaaaa
      301 caaaattcgg ctcggcggcg tggcgctaat ggccggcgca tcgatcgcag aaagcgcgct
      361 tctttcccta aaaacggttt gcgcgcgaag cggcgcgggg ttctaccttc tgtctgctcg
      421 cgtggcggtt gtcgtcgcgc aggcgtcccc acagctcttc aaccttattc actatgcctc
      481 tcgcaatcgg ttccaaagcc ccggatttca cgctcaagac caagacggcc gatggcctca
      541 aggatatcaa gctgagcgac aacttcggcc agcagcagac ggtgctgttg ttcttcccgc
      601 tggcgttcac cggcgtgtgc acgcaggaga tgtgcgatca gagcgcaggg cttggcgatt
      661 atgagaagtt gggcgcgaag gtgatcgcga tcagcgtcga cagcccgttc gcccaagagg
      721 cttgggcgaa agcgaacaag atttccctca cgatcgtcag cgatctcaac aaggaggtca
      781 cgaaggctta cgacgtggtg ttcccgatgc tggccggagt cggtgatacg gcggcccgcg
      841 cggcgttcgt catcggcaag gatggtgtga tcaaatacgc cgagcagacg ccgacgccga
      901 aggatctgcc gaatttcgcc gcggtgaagg cggcgttggc gaagtaaatt aagcgagttg
      961 tgatcactgt ggcgcggcga gcgcgccacc tactatttct aacatgagtc ttccgaatcc
     1021 gttcaacacg ctgcagacgt tctcggccaa cggggcgacg cacccattct actcgattcc
     1081 cgcgctcgaa ggcgcgggct tccaagtcaa gaagctgccg gtctcgatcc ggctcgtgct
     1141 cgagtcgctc ctgcgcaat
//
LOCUS       DASPLT010001130         2559 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_73647, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001130 DASPLT010000000
VERSION     DASPLT010001130.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 2559)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2559)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2559
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_73647"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..283)
                     /locus_tag="VEA63_11620"
     CDS             complement(<1..283)
                     /locus_tag="VEA63_11620"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HYD84699.1"
                     /translation="MKRAKPDLDDADRRLLRAIQRDASASQTDLAEAAGVSPSLVSRR
                     LSRLKEAGVLRSIVGLIAPEEVGLTCSAIIRVRLKDHAAASVKAFRELVA"
     gene            383..2461
                     /locus_tag="VEA63_11625"
     CDS             383..2461
                     /locus_tag="VEA63_11625"
                     /inference="COORDINATES: protein motif:HMM:NF012880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HYD84700.1"
                     /translation="MSAKPKPAAKTVPQTDWKRVVELVQTSRAMDAIEERELVPGKKI
                     FYQFSARGHDMAQVLLGLHLTHPKDAMCGYYRSRPILLSLGVALEDALGSAMGRAGGY
                     SDGRDIGVVFNYPNATGASALPMCGGVGAQYTPTAGYAQAIEYHKNVLGDRAYDGAIG
                     VVLGGDASVATNGFWSALTMATTLKLPMLFYVEDNGYGISTPGWLQTPGCNIAQNLAS
                     FGGLKICDGDGTDPVEASELIADAVAHVRGGGGPALLRLTVPRLQGHSFQDTQAYKSK
                     EVVDAEWARDPLPRLKTHVVPSLMSEKEWDAAETQAEERVRRAAQIADQRPVSEASKV
                     TRFVFSEENALQDEGGLWNGGYAAPRVGDVAKPEGARINMITAIRRTLDHELAVNPRV
                     LVFGEDVGPKGGVHGVTLGLQEKYGDGRVFDTSLSEEGIIGRAVGMAIAGLMPVPEIQ
                     FRKYADPATEQINDCGTMRWRTVNRFAAPMVVRMPVGFFKCGDPWHSQTNEVQFVHSP
                     GWRVACPSNAEDAVGLLRTALRGNDPVMFLEHRNMLDAASARRPYPGDEFALPFGVAK
                     RVREGSDITLVAWGAMVERCEAAADKAGVSADILDLRTLAPWDSEAVLASVRRTHRCF
                     IVHEDIGTAGFGAEIAAVVADQAFLDLDAPVARMTMPDIPSPHHPELMEWALPSVERI
                     AAKIEELVRF"
BASE COUNT          466 a          747 c          915 g          431 t
ORIGIN      
        1 gggcgacaag ctcgcggaac gctttgacgc tggcggcggc gtgatccttc aggcgcacgc
       61 ggatgatggc cgagcacgtc agccccacct cctccggcgc gatcaggccg acgatgctgc
      121 ggagaacacc cgcctctttc agacggctaa ggcggcggga gacgaggcta ggcgagacgc
      181 cagcggcctc ggccaaatcc gtttgggagg cggatgcgtc gcgctgaata gcgcgcaaca
      241 ggcggcggtc cgcatcgtcc aggtcaggct ttgcacgctt catgcggatt ccatatcata
      301 actgcattaa ctgggcaatt agagcaccaa gactgcctcc tttatcacgg aaattgccga
      361 caagcgctag tattgtgcgc ggatgagcgc caagccgaag ccagccgcca aaaccgtccc
      421 acagaccgat tggaagcgcg tagtcgaact cgtccagacc tcccgcgcga tggacgcgat
      481 cgaggagcgc gagctcgttc cgggcaagaa gatcttctat cagttcagcg cccgcggaca
      541 cgacatggcg caggtgctgc tgggcttgca cctgacgcac ccgaaagacg cgatgtgcgg
      601 ttactaccgc tcgcggccga tcctactttc actgggcgtg gcgcttgagg acgcgctggg
      661 ttcggcgatg gggcgcgcgg gcggctatag cgacggacgc gatatcggcg tcgtgttcaa
      721 ttatccgaac gcgacgggcg catctgccct gccgatgtgc ggcggcgtcg gcgcgcaata
      781 cacgccgacg gcgggctacg cgcaggcgat cgaatatcac aagaacgtct tgggcgaccg
      841 cgcctatgac ggcgcgatcg gcgtcgtgct cggcggcgac gcttcagtgg cgacgaacgg
      901 gttctggtcg gcgctgacga tggcgacgac gctgaagctg ccgatgctgt tttacgtcga
      961 ggacaatggc tacggcatct caacacccgg ctggctgcaa acgccgggct gcaacatcgc
     1021 gcaaaatctg gcgagcttcg gcggactgaa gatttgcgat ggcgacggga ccgatccggt
     1081 agaggcgtcc gagcttattg cggatgccgt agcgcatgtg cgcggcggcg gtgggcctgc
     1141 tctgctgcgc ctcactgtgc cgcggctgca agggcactcg tttcaggaca cgcaggcgta
     1201 caagtcgaaa gaggtcgtcg acgcggaatg ggcgcgcgat ccgttgccga gactgaagac
     1261 gcatgtcgtg ccgtcgctga tgagcgagaa ggaatgggac gcggctgaaa cgcaagcaga
     1321 ggagcgcgtg cggcgggcgg cgcagatcgc cgatcagagg ccggtgagtg aagcttcgaa
     1381 ggtgacgcgc ttcgtgttca gcgaagaaaa cgcgctgcag gacgaaggcg ggctgtggaa
     1441 cggcggctat gccgcgccgc gtgttggcga tgtggcgaag cccgaaggcg cgcgcatcaa
     1501 catgatcacg gcgatccggc ggacgctgga tcatgagctt gctgtgaacc cgcgcgttct
     1561 ggtgttcggc gaagatgtcg gcccgaaggg cggcgtgcat ggcgtgacgt tgggcctgca
     1621 ggaaaagtac ggcgatggcc gcgtgttcga cacgtcgctg tcggaggaag gcattatcgg
     1681 ccgcgccgtc ggcatggcga tcgcgggatt gatgcccgtg cccgaaatcc agttccgcaa
     1741 gtacgccgat ccggcgacgg agcagatcaa cgattgcggc acgatgcgct ggcgcacggt
     1801 gaaccggttt gcggcgccga tggtggtgcg catgccggtc ggcttcttca agtgcggcga
     1861 tccttggcat tcgcagacca acgaagtgca gttcgtgcat tcgcccggat ggcgtgtcgc
     1921 ctgcccttcg aacgcggagg acgcggtggg cctcttgcgc acggcgctgc gcggcaacga
     1981 cccggtgatg ttcctcgaac accgcaacat gctcgacgcg gcgagcgcgc gacggcccta
     2041 tccgggtgat gagttcgcgc tgccgttcgg cgtggccaag cgcgtgcgcg agggcagcga
     2101 cattacgcta gtggcctggg gcgcgatggt ggagcgctgc gaagcggcgg cggataaggc
     2161 tggcgtcagc gccgatattc tggatctgcg tacgctggcg ccgtgggatt ccgaagcggt
     2221 gttggcctcg gtgcggcgca cgcatcgctg tttcatcgtg catgaagata tcggcaccgc
     2281 gggcttcggc gcggagatcg cggctgtagt ggcggaccag gcgtttctcg atctcgacgc
     2341 gccggtggcg cggatgacga tgccggacat cccctcgccg caccatcccg aactgatgga
     2401 atgggcgctg ccttcggtgg agcgcatcgc ggcgaagatc gaagagctgg tgcggttctg
     2461 atggcggagc aacaagacat catcgcccct gtcgaacagg aaggcaccaa gtcggtcgtg
     2521 cgcgcgtggc tgaagcagat cggcgacgcg gtgaagcaa
//
LOCUS       DASPLT010001131         1778 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_73649, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001131 DASPLT010000000
VERSION     DASPLT010001131.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1778)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1778)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1778
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_73649"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..846)
                     /locus_tag="VEA63_11630"
     CDS             complement(<1..846)
                     /locus_tag="VEA63_11630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD84701.1"
                     /translation="MGFFDRFSEKKLSSTPPSNGTPETNVGGIMSRLITARERLEAND
                     LPAALAIYEDVLATSGDRADVLVTISADLGSNGHVREIVELIAPRYDAERHGPATGLN
                     LLQAYLVEKNPDAAQHVLDILFGLKRPELEERLYGFSNAIAELIHSQQTAVSPPGGQG
                     PAAEPAKVNLVSISKPMWFYGLEPLAAEILPPKSQRLRRVAFAQLATPGLAEFAEIAK
                     QPENELGRLSRAIPLWFAETFYFSPHYSPLAAVGIIEAHYALFPIEWTADNLRQLIAS
                     TEGGVD"
     gene            complement(956..>1778)
                     /locus_tag="VEA63_11635"
     CDS             complement(956..>1778)
                     /locus_tag="VEA63_11635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="BadF/BadG/BcrA/BcrD ATPase family protein"
                     /protein_id="HYD84702.1"
                     /translation="NPNIVGQDQARLIVTDALCALLAQANANAAAGAGPVRVALTRLY
                     MSGSPNFWREFAASLTDFGDVATAPDSLPVLELATDGEPGLVVHSGTGSFVAARDPSG
                     GIHYAGGLGWRFGDPGSGYDLGRRAISRALLELQGWLPPSRLGPTVRDHTQLGALADA
                     ATLTRFFYSEPLPNQKIAALAPAVLRLASEGDHTAHTLIVESAVELLELAARVAAKLF
                     PAIPADLLRAGLSGPILTHPVVREALAPRSPLPLVPITAAPIEGVRRLLVRLSAP"
BASE COUNT          336 a          568 c          599 g          275 t
ORIGIN      
        1 atcgacgccg ccttcggtgc tcgcgatcaa ctggcgcaga ttgtcggccg tccactcgat
       61 aggaaacagc gcgtagtgcg cttcgatgat cccgaccgcg gcgaggggcg aataatgcgg
      121 cgaaaaataa aatgtctcgg cgaaccacag cggaatcgca cggctcagcc ggcccagctc
      181 gttctcgggt tgcttcgcga tctcggcaaa ctcggcgagg cccggcgtcg cgagttgggc
      241 gaaggcgacg cgccgcagcc gctggctttt cggcggaagg atttccgcgg cgagcggctc
      301 gagtccgtag aaccacatcg gcttgctgat gctcacgagg ttgaccttcg ccggttcggc
      361 ggcgggacct tggccaccgg gaggagagac cgctgtttgc tgcgaatgaa tcagctcggc
      421 gatcgcgttc gaaaatccat acaaccgctc ctcgagctcc gggcgtttca acccgaacag
      481 gatgtcgagc acgtgttgcg cggcgtccgg atttttttcg acgagataag cctgcagcaa
      541 attcaagccg gtggccgggc catgacgctc ggcatcgtaa cgcggcgcga tgagttcgac
      601 gatctcgcgc acatggccgt tgctcccgag atcggcggag atcgtcacca acacatccgc
      661 ccggtcaccg gaggtggcga gcacatcctc gtagatcgcg agcgcagcgg gcagatcgtt
      721 cgcctcgagt cgttcgcgcg ccgtgatgag ccgcgacatg atgccaccga cgttcgtctc
      781 gggcgtgcca tttgatggcg gcgtggacga cagttttttc tcggagaagc gatcgaagaa
      841 tcccatgcgg cgaggaaaac gccgggcgtc gctctggcga acagttttcc gctaaaatcc
      901 ccggcagatg cgccggccgc gtgagccacg aacgacacgc gctacccgcc agggactacg
      961 gcgccgacag ccgcacgagc aagcgacgca cgccttcgat cggcgcggcg gtgatcggca
     1021 cgagcggcag cggcgaacgc ggtgcgagcg cctcgcgaac gaccgggtgc gtgaggatcg
     1081 gtccactcag cccggcgcgc agcaggtcgg ccggaatcgc cggaaaaagt ttcgccgcca
     1141 ctcgcgcggc aagttcgagg agttcgaccg cggattcgac aatcaacgtg tgcgccgtgt
     1201 ggtcgccttc gctcgccaga cgaagcaccg caggcgccag cgcggcgatc ttctggttgg
     1261 gcaacggctc gctgtaaaaa aaacgcgtga gcgtggcagc atccgccaac gcaccgagtt
     1321 gagtgtgatc gcggaccgtg gggccgagcc gcgacggcgg cagccagccc tggagttcga
     1381 gcaaagcgcg cgagatggcg cggcgtccga gatcgtagcc gctgcccgga tcgccaaaac
     1441 gccagccgag cccgcccgca taatgaattc cgccggacgg atcgcgcgcg gcgacgaacg
     1501 agccggtgcc ggaatgcacc acgagccccg gctcgccatc cgtggcgagc tcgagcacgg
     1561 gcagcgaatc gggtgccgtc gccacgtcgc cgaaatcggt caacgacgcg gcaaactcgc
     1621 gccaaaaatt cgggctgccg gacatgtaga gacgcgtcag cgcgacgcgc accggaccgg
     1681 cccccgcggc tgcgtttgcg tttgcttgtg cgagcagagc gcaaagcgca tcggtcacga
     1741 taagccgcgc ctggtcctgg ccgacgatgt tcgggttg
//
LOCUS       DASPLT010001132         1188 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_53095, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001132 DASPLT010000000
VERSION     DASPLT010001132.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1188)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1188)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1188
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_53095"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1188
                     /gene="sppA"
                     /locus_tag="VEA63_11640"
     CDS             <1..>1188
                     /gene="sppA"
                     /locus_tag="VEA63_11640"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007361161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HYD84703.1"
                     /translation="TALETFKESGKPVKGYLTYATTKNYYLASAADELALDPYGLLIM
                     PGLSAQPMFLAGAFEKYGIGVQVTRVGKYKSFIEPFTRTDMSPESRAQMQKLLDDLWA
                     GLIGDMGATREVRPAAIQRMVDTEGVIQPEAALAAKLVDKIAYRDQVIDELKQATGPG
                     GDHRSFKQITMVDYAKIAKDTVEKPKRRADADKDESEPAKATGRTGKIAIVYAEGDLV
                     DGEGEQPGEIGGTRFARELRRLREDGNVKAIVLRVNSPGGSASASETIQREVRLARET
                     KPVIVSMGSYAASGGYWIATHGDWIFAEPTTVTGSIGVFGIQFDVQRLANDFGLTFDS
                     VKTGRMADMITVARPKTPEEMAVYQRLVDRIYDQFITRVAEGRKLERSFVEEIAQGRV
                     WSG"
BASE COUNT          248 a          308 c          448 g          184 t
ORIGIN      
        1 gcacggcgct cgagacgttc aaggaatcgg gcaagccggt gaaaggttat ctcacttatg
       61 cgacgacgaa aaactactat ctcgcgtcgg cggccgacga actcgcgctt gatccgtatg
      121 ggttgctgat catgccgggc ctctcggcgc agccgatgtt tttggcgggg gcgttcgaga
      181 aatacgggat cggcgtgcag gtgacacgcg tgggaaaata caaatcgttc atcgaaccat
      241 tcacgcggac ggacatgagc ccggagagtc gcgcgcagat gcagaagctg ctcgacgact
      301 tgtgggcggg attgatcggc gacatgggcg cgacgcgcga agtgcggccg gcggcgatcc
      361 agcgcatggt cgataccgaa ggtgtgattc agccggaggc ggcgctcgcg gcgaagctcg
      421 tcgataagat cgcgtatcgc gatcaagtga tcgacgaact gaaacaggcc acggggccgg
      481 gcggagacca ccgcagcttc aagcagatca cgatggtcga ctacgcgaag atcgcgaagg
      541 acacggtgga aaaaccgaag cggcgcgcgg acgccgacaa agacgagagc gaaccggcga
      601 aggcgacggg ccgcacaggg aagatagcga tcgtgtatgc ggagggcgac ttggtcgacg
      661 gcgagggcga gcaaccgggc gagatcggcg gcacgcgttt cgcgcgcgag ctgcggcggc
      721 tgcgcgagga cgggaacgtg aaagcgatcg tgttgcgcgt gaacagtccg ggcgggtcgg
      781 cgtcggcttc ggaaacgatt caacgcgaag tgcggctcgc gcgggagacg aagccggtga
      841 tcgtttcgat gggctcgtat gcggcgtcgg gcggctactg gatcgcgacg cacggcgact
      901 ggattttcgc ggagccgacg acggtcacgg gatcgatcgg cgtgttcggg attcagttcg
      961 acgtgcagcg gctcgcgaac gatttcgggc tgacgttcga cagtgtgaaa acaggccgga
     1021 tggcagacat gatcacggtg gcgcggccga agacgccgga ggaaatggcg gtgtatcagc
     1081 ggctggtcga ccggatctac gatcaattta tcacgagggt cgcggagggc cggaagctcg
     1141 agcgcagttt cgtcgaggag atcgcgcagg gccgcgtgtg gtcgggcg
//
LOCUS       DASPLT010001133         1609 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_136814, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001133 DASPLT010000000
VERSION     DASPLT010001133.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1609)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1609)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1609
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_136814"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..592)
                     /locus_tag="VEA63_11645"
     CDS             complement(<1..592)
                     /locus_tag="VEA63_11645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD84704.1"
                     /translation="MKVRSLLAAALAAVATNAAFSADVNVSGKISTSTTWTSSNTYIL
                     EGYVFVMPGATLAIQPGTVVKAKQSAGANAAALVITAGAKIDANGTAQNPIIFTSELD
                     NLNGNLTHQDTALWGGVAILGKASINSRANSAVAAAPVQDQIEGFSVTGDDNALITFG
                     GTDDADNSGTFRYVSIRHGGAVIGTANEINGLTLGGV"
     gene            complement(636..>1609)
                     /locus_tag="VEA63_11650"
     CDS             complement(636..>1609)
                     /locus_tag="VEA63_11650"
                     /inference="COORDINATES: protein motif:HMM:NF026255.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HYD84705.1"
                     /translation="AGYAMVDAQLTEKLRAIGGVRFESTEMRTDPVPVVGGSPKLGII
                     DQVDTLPALSLVYASSPKTNWRLAYGRTVARPTYKELTDIRYEDVFTGDVYLGNAELD
                     LTLIDNLDLRWEWFPRRGETLAVSAFYKRLDQPIEVLYDPGVGSIQPQNVERGTVYGL
                     EFEFRRSLQFLGDAFANFSVGTNLTFVHSEVTIPERELAILRAYDPNADDKRELLGQS
                     PYIFNFDVSYDRASSGTAATLSFNVVGERLDLVNFGPLPDVFEQPAPLLNLVVAQRLS
                     DRWRLKLSVKNLLDPDHEKTIAVSGQTLIYERSSVGRSYAIGVNYAF"
BASE COUNT          306 a          506 c          489 g          308 t
ORIGIN      
        1 caaccccgcc gagagtgagg ccgttgattt cgttcgccgt gccgatcacc gcgccgccgt
       61 ggcggatcga aacgtaacgg aacgtgccgg agttgtccgc atcgtccgtg ccgccgaacg
      121 tgatcagcgc gttgtcgtcg ccggtgaccg agaaaccttc gatttgatcc tgcaccggcg
      181 cagccgccac ggcgctattc gcgcgcgagt tgatgcttgc cttgccgaga atcgcgacgc
      241 cgccccacaa cgccgtgtcc tgatgcgtga gattgccatt caggttgtcc aactcggacg
      301 tgaagatgat cgggttctga gccgtgccgt tggcgtcgat cttcgcgcca gcggtgatca
      361 cgagcgccgc ggcgttggcg ccggcgctct gtttcgcttt gaccaccgtg ccaggctgga
      421 tcgccagcgt ggcacccggc atcacgaaga cataaccctc gagaatatag gtgttgctgc
      481 tggtccacgt ggtgctcgtc gaaatttttc cggagacatt aacgtccgcc gaaaaggcgg
      541 cgttggtggc aacggccgcg agcgccgccg cgagtaagga tcttactttc atgtttttca
      601 ttttggttgg gtttagggag aggaaattcg gcgggttaaa acgcgtagtt cacaccgatc
      661 gcatagctgc gaccgaccga gctgcgctcg tagatcagcg tctgcccaga caccgcgatc
      721 gtcttctcgt gatctggatc gagcaggttt ttgacgctca acttcaaccg ccagcgatcg
      781 gacagtcgct gcgccaccac gagattgagc aacggagccg gctgctcgaa cacgtcgggt
      841 aacgggccga agttcaccaa gtcgagccgc tcgccgacga cgttgaacga cagcgtcgcc
      901 gccgtgccgg agctcgcgcg atcgtagctg acatcgaagt tgaagatgta aggcgactgc
      961 ccgagcaact cgcgtttgtc gtccgcgttc ggatcgtaag cgcgcaggat cgcgagctcc
     1021 ctctccggaa tcgttacctc ggagtgaacg aacgtgagat tcgtgccgac ggagaaattc
     1081 gcgaacgcat cgccgagaaa ttgaaggctg cggcggaact cgaactcgag tccatagacc
     1141 gtgccgcgct cgacattctg cggctgaatc gagccgacgc ccgggtcgta aagcacctcg
     1201 atcggctgat ccaaccgctt gtagaacgcc gacaccgcca gcgtctcgcc gcgccgcgga
     1261 aaccactccc agcgcaaatc gagattgtcg atcaacgtca ggtcgagctc cgcgttgccc
     1321 aggtaaacat cgccggtgaa cacgtcctcg tagcgaatat ccgtcagctc tttgtaggtc
     1381 gggcgcgcca ccgtgcggcc atacgcgagc cgccagttcg tcttcggcga gctcgcgtaa
     1441 acgaggctca gcgcgggcag cgtatcgacc tggtcgatga tgccgagctt cggcgaaccg
     1501 ccgactaccg gcaccggatc cgtgcgcatt tccgtcgact cgaaacgcac gccgccgatc
     1561 gcacgcagct tctccgtcag ttgcgcatcc accatcgcgt agccggcgg
//
LOCUS       DASPLT010001134         2000 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_259321, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001134 DASPLT010000000
VERSION     DASPLT010001134.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 2000)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2000)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2000
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_259321"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..277
                     /locus_tag="VEA63_11655"
     CDS             <1..277
                     /locus_tag="VEA63_11655"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HYD84706.1"
                     /translation="YRITQEAIHNATRHGGARRIVVTLNSRGKEHRLSIRDDGKGFDA
                     EAPGTATGAGLRLMSYRANMIGGTFSIESAPQRGTQVNVLFTTVSSK"
     gene            274..981
                     /locus_tag="VEA63_11660"
     CDS             274..981
                     /locus_tag="VEA63_11660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012372872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HYD84707.1"
                     /translation="MKTISDPDTTTTSPTPTPTKSRIFLVDDHPITRQGVAVLINQER
                     DLTVCGEADSAPVAIDMIQKLNPDVALVDITLKTTSGIELMKHLKALRPEMPVLIMSM
                     HDESLFAERALRAGAKGYIMKHEASAKILTAIRRVLSGELYLSDKMKEKMLHRLVNNR
                     KEEVVFSIDTLSDREMEVFQLIGNGYGTRQIASKLNLSVKTIDSYREHLKLKLRLESG
                     TELVRHAIQWVKSEGIV"
     gene            1274..>2000
                     /locus_tag="VEA63_11665"
     CDS             1274..>2000
                     /locus_tag="VEA63_11665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012372871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HYD84708.1"
                     /translation="MKSSPKQKIRPEQTQAHQPGIESVMTPRPVSENHSQRGSGRLSG
                     KTALITGGDSGIGRAVALAFAKEGANVSIGYLDEQSDAVETRKRVEGYGRTCLLMPGD
                     VGDSQECERMVTATLAKFGGVDILVNNAAEQHPQDSIEKISDAQLERTFRTNFFAYFY
                     LTRATLPYLKKGASIINTTSVTAYRGSPQLLDYSATKGAIVAFTRSLALAVAEQGIRV
                     NAVAPGPIWTPLIPATFSKDKVAK"
BASE COUNT          426 a          580 c          618 g          376 t
ORIGIN      
        1 ttaccgcatc acgcaggagg cgatccacaa cgccacacgg cacgggggcg cgcgacgcat
       61 cgtggtgacg ctgaactcgc gcgggaagga acaccggctt tcgatcaggg acgatgggaa
      121 gggcttcgac gccgaggccc cgggaaccgc aaccggcgcg ggattacgat taatgagcta
      181 tcgggctaac atgatcggtg gaacattttc catcgaatct gccccgcagc gcggaacaca
      241 ggtcaacgtt ctgtttacga cagtctcatc gaaatgaaaa cgatctccga ccccgatacc
      301 acgacgacgt ctccgacccc gactcccaca aagagccgga tattcctcgt cgacgatcat
      361 ccgatcacgc ggcaaggcgt cgccgtgttg atcaatcaag agcgcgatct gacggtttgc
      421 ggcgaggcgg acagtgcgcc ggtcgcgatc gacatgattc aaaagctcaa tcccgatgtg
      481 gctttggtcg acatcacgct gaaaacgacg agtggcatcg agctgatgaa gcacctcaag
      541 gcgcttcgtc cggagatgcc ggtgctgatc atgtcgatgc acgacgaaag cttgttcgcc
      601 gagcgggctc tgcgcgcggg cgccaagggc tacatcatga agcacgaggc gagcgcgaaa
      661 attctcacgg cgatccgccg cgtgctctcg ggcgagttgt atctgagcga caagatgaag
      721 gagaagatgc tgcaccggtt ggtgaacaac cggaaggaag aggtggtgtt ctccatcgat
      781 accttgagtg atcgcgagat ggaggtgttt caactcatcg gcaacggcta cggcacgcgg
      841 caaatcgctt cgaagctcaa tctcagcgtc aagacgatcg attcctaccg cgagcacctg
      901 aagctcaagc ttcggctgga aagcggcacg gagctggtgc gccacgcgat ccagtgggtg
      961 aagagcgaag gtattgtttg aacgatcgcg aaccgttcgc tggcggtgct gtccgtgaaa
     1021 ctcctccggt gcctcgcgcc gtgatccctc cgatgtgcag tcggagcgga ttctgatatt
     1081 tctcttttca gtgtttcggt ggtcgggctg cctcacaccc ctgtcgaccc ttatgtcggc
     1141 atgaggcggc ccggccatcg agtctgactc tcttcttcgt aagaacccca tcagggttac
     1201 cccggcgagg cggtcatagg tttgcctgat ttcaagcggc tggcgaaacg ggcacaatgt
     1261 ggctgtccgc cacatgaagt cgtctccgaa acaaaaaatc cgcccggagc aaacccaggc
     1321 tcatcaaccc ggcatcgagt ccgtgatgac tccgcggccg gtgagcgaaa atcacagtca
     1381 gcgaggcagc ggccgtttgt cggggaaaac cgcgctgatc acgggcggcg atagcggcat
     1441 cgggcgcgcg gtggcgctcg cgtttgccaa ggagggtgcg aacgtctcca tcggttatct
     1501 cgacgagcag tccgacgcgg tcgagacccg caaacgcgtc gaaggctatg gccgcacgtg
     1561 cctgttgatg ccgggcgacg tcggggattc tcaggagtgc gagcggatgg tgacggcaac
     1621 gctggcgaag tttggcggcg tcgacattct cgtgaacaac gccgcggagc agcatccgca
     1681 ggattcgatc gaaaagatct ccgatgccca gcttgagcgg acgtttcgca cgaacttctt
     1741 tgcgtatttt tacctgacgc gcgcgacgct gccttacctg aaaaaaggtg cgtcgatcat
     1801 caacaccacg tcggttacgg cttatcgcgg cagccctcaa ctgctggact acagcgcgac
     1861 gaagggagcg atcgtggcct tcacgcgctc gcttgcgcta gcggtcgccg aacagggcat
     1921 acgcgtgaac gcggtcgctc ccggaccgat ttggacgccc ttgattcccg cgacattttc
     1981 gaaggacaag gtcgcgaagt
//
LOCUS       DASPLT010001135         1033 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_73662, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001135 DASPLT010000000
VERSION     DASPLT010001135.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1033)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1033)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1033
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_73662"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(143..>1033)
                     /locus_tag="VEA63_11670"
     CDS             complement(143..>1033)
                     /locus_tag="VEA63_11670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012377113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 8"
                     /protein_id="HYD84709.1"
                     /translation="RIWRWAIKHMRHADGPRRGYFAWHCTFDGRQIDVGSASDGEEWI
                     AMALFFAAHRWNDAHYLTEARSLLHEMLHKPATDVVTPIFDRAEHQVVFAPTPRASQL
                     TDPSYHLPHYYELWARWANDIEDRTFWKTAAETSRAFFRRAAHPGTGLMPEYAHFDGR
                     PFLETTFGPGKGDFRFDAWRTLAHVALDHAWFGADLWQVEQSNRVLRFLLSHEPRIPN
                     QFALDGTPLSEDTSSGLIAMAAVAGLAADPELARPFVQQLWDAPIPTGKWRYYNGLLY
                     HLALLQVSGRFQIYDAPDAR"
BASE COUNT          188 a          301 c          364 g          180 t
ORIGIN      
        1 gtcgacgccg ccgtcggtag tgaagcgcca gctcatccta cagcgggcgt ccagatttca
       61 tgcgatgtta ccacgctacg tggtagcgtg tccccacgcg cctgggcgat cgcgcaacga
      121 gggcgaccgg cgacagccgc ccctaccggg cgtctggcgc gtcatagatc tggaatcggc
      181 cgctcacctg aaggagcgcg agatggtaga ggagtccgtt gtagtagcgc cattttcccg
      241 tgggaatcgg agcgtcccag agttgttgca cgaaggggcg agcgagctcg ggatcagccg
      301 cgagtccggc gacggcggcc atcgcgatca atccgctgga ggtgtcttcg gacagcggcg
      361 tgccatcgag tgcgaactgg ttcgggatgc gcggctcgtg cgagaggaga aaacgtagca
      421 cgcgattact ctgctcgacc tgccagaggt cggcgccgaa ccacgcgtga tcgagtgcga
      481 cgtgcgcgag cgtgcgccag gcatcgaagc gaaagtcgcc cttgcccgga ccgaaggtgg
      541 tttcaaggaa cggccgtccg tcgaaatgcg catactccgg catgagcccc gtgcccgggt
      601 gcgcagcgcg gcggaagaac gcgcgactcg tttcggcggc ggttttccag aacgtgcgat
      661 cttcgatgtc gttcgcccag cgcgcccaaa gttcgtagta gtgcggaaga tgatacgagg
      721 ggtccgtcag ttgactggcg cgcggcgtgg gtgcgaagac gacttgatgt tcggcgcgat
      781 cgaaaatcgg ggtgacgaca tcggtcgccg gcttgtgcaa catttcgtga agaagcgacc
      841 gtgcttccgt gaggtagtgt gcgtcattcc agcggtgcgc ggcgaagaag agggccatgg
      901 cgatccactc ttcgccgtcg gaggcggagc cgacgtcgat ctgacgaccg tcgaacgtgc
      961 aatgccacgc gaagtagccc cggcgcggcc cgtcggcgtg gcgcatgtgt tttatcgccc
     1021 aacgccagat gcg
//
LOCUS       DASPLT010001136         1006 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_259326, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001136 DASPLT010000000
VERSION     DASPLT010001136.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1006)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1006)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1006
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_259326"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..216
                     /locus_tag="VEA63_11675"
     CDS             <1..216
                     /locus_tag="VEA63_11675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD84710.1"
                     /translation="SVLDRSVLNRFTLFGKTKEQRAREAEAIEQNARVLGEIAELIEF
                     SQKGGGEAEEERKLRELYLDVFVSRPR"
     gene            complement(589..>1006)
                     /locus_tag="VEA63_11680"
     CDS             complement(589..>1006)
                     /locus_tag="VEA63_11680"
                     /inference="COORDINATES: protein motif:HMM:NF037435.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF5616 domain-containing protein"
                     /protein_id="HYD84711.1"
                     /translation="SALAGGVVLLGRDGCCRDMASLHGTYRQVEETLRAAELIGTFAV
                     THHVTRCRWLLDRPVNNSGRLKTLLLDLAEKFNWPWDVELEFSPDTSLSQTTAIVATS
                     DSVILDRCEQWCNAAHEIIAASLPETPIIDLRPAPC"
BASE COUNT          218 a          242 c          367 g          179 t
ORIGIN      
        1 tcggtgctcg atcgcagcgt gctgaaccgg ttcacgcttt ttggtaagac gaaagaacag
       61 cgcgcgcgcg aggcggaggc gatcgagcag aatgcgcgcg tgttgggaga gatagctgag
      121 ttgatcgagt tcagccagaa aggcgggggc gaggcggagg aggagcggaa gctgcgcgag
      181 ctttatctcg atgtgttcgt gtcgcggccg cggtgaggtg gagggtggaa agcgcgcgcg
      241 gacgaaccgg gatcgatcac accgaaagga gaccacagca tggaacacag aggacacgga
      301 gcgatgacac agaggtcacg gagcaaggca gggtgttcgg ctcgatggac gcggtgtggg
      361 gccgtgcact cttagccgga gcaattcagt aggaaaccac cgatgaacac ggatggacac
      421 ggattgaaaa ccctttccac aaaaggaagg atcatttgaa aaactcactc tttggtgacg
      481 ataaacccgt cgcatttagc ccgggaactt cacacctgta gcgaaacgct cacgcgtttc
      541 gctacaacag actgcgccca cctcgcatct gcgtgtgatg aatgcgggtt agcagggggc
      601 ggggcggagg tcgattatcg gtgtttcggg gagacttgcg gcgatgattt cgtgggcggc
      661 gttgcaccac tgttcgcagc ggtcgaggat gacgctgtcg gaggtcgcga cgatcgcggt
      721 cgtttggctg agcgaggtgt cgggactgaa ttcgagctcg acgtcccaag gccagttgaa
      781 tttctcggcg agatcgagga ggagagtttt cagacggccg ctgttgttga cggggcgatc
      841 gaggagccag cggcagcggg tcacgtgatg cgtgacggcg aaagtgccga tgagttcggc
      901 ggcgcggagg gtttcttcga cttggcgata cgtgccgtga aggctcgcca tgtcgcggca
      961 gcagccgtcg cggccaagga gaacaacgcc gccggcgaga gcagat
//
LOCUS       DASPLT010001137         2192 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_136894, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001137 DASPLT010000000
VERSION     DASPLT010001137.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 2192)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2192)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2192
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_136894"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1430
                     /gene="sdhA"
                     /locus_tag="VEA63_11685"
     CDS             <1..1430
                     /gene="sdhA"
                     /locus_tag="VEA63_11685"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007359765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="succinate dehydrogenase (quinone) flavoprotein
                     subunit"
                     /protein_id="HYD84712.1"
                     /translation="QLLLGAYSALSRMIGAGAVQLFANTEMLDLVIVNGQAKGIVARD
                     LITGEITRHAADAVILATGGYGNVFNLSTYARQSNATAIWRAYKRGACFANPCYTQIH
                     PTCIPVTGDYQSKLTLMSESLRNDGRIWVPKKAEDCRKDPNAIPEADRDYYLERMYPS
                     FGNLAPRDIASRAAKRVCDEGRGVGETGLGVYLDFSDAINRLTEAGVRERYGNLFDIY
                     HEITNENAYKVPMRIYPAVHYTMGGLWVDYNLMSNLPGLFVLGEANFSDHGANRLGAS
                     ALMQGLADGYFVIPYTVGDYLATQKPGSRPSADAAEFKAAEQNVTGLTSRLLGINGKE
                     PVSHFHKRLGKIMWEGCGMARTAATLTKALQEIPKLREEFWANVKVPGSAATLNQSLE
                     QAGRVADFLELGELMCLDALTREESCGGHFREEFQYPDGECKRDDEKFSHVAAWEFQG
                     DGKAPLRNVEPLNYEFVKPSVRSYK"
     gene            1514..>2192
                     /locus_tag="VEA63_11690"
     CDS             1514..>2192
                     /locus_tag="VEA63_11690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007359766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     iron-sulfur subunit"
                     /protein_id="HYD84713.1"
                     /translation="MVAATSSKNISITLRVWRQANPSAPGKFVDYPASNLNSNMSFLE
                     MLDVVNDELTKKGEEPIAFAHDCREGICGTCSLTINGKPHGPGQGIATCQTYMRSFHD
                     GDVIVVEPFRARAFPLVKDLVVDRSAFDKIQQAGGFISVRTGAAPDANSIPIPKGDAD
                     LAMDAAACIQCGACVAACKNASAMLFVAAKVSHLGLLPQGQPERDRRVLAMVNTMDEL
                     GFGSCTNQ"
BASE COUNT          417 a          597 c          760 g          418 t
ORIGIN      
        1 aacaattgct gctcggcgcg tattcggcgt tgtcgcggat gatcggtgcg ggcgcggtgc
       61 agttgttcgc caacaccgag atgctcgatc tggtgatcgt caacggccag gcgaagggca
      121 tcgttgcgcg cgacctcatc accggggaga tcacgcggca cgcggcggat gcggtgattc
      181 tcgcgacggg tggctacggc aacgtgttta atctctcgac ctatgcgcgg cagtcgaacg
      241 cgacggcgat ctggcgcgct tacaagcgcg gcgcgtgttt tgcgaatccg tgttacacgc
      301 agattcaccc gacgtgcatt ccggtgacgg gcgactatca gtcgaagctt acgctgatgt
      361 cggagtcgct gcgcaacgac ggccgcatct gggtgccgaa gaaggcagag gattgccgga
      421 aggatccgaa cgcgattccg gaagcggatc gcgactacta tctcgagcgg atgtatccaa
      481 gttttggtaa cctggcgccg cgcgatatcg cgtcacgcgc ggcgaagcgc gtgtgcgacg
      541 aaggtcgcgg cgtgggcgag acggggctgg gcgtgtatct ggatttctcc gacgcgatca
      601 accggttgac cgaggcgggc gtgcgtgagc gttacggcaa cctcttcgat atttatcacg
      661 agatcacgaa cgagaacgcc tacaaggtgc cgatgcggat ttatccggcg gtgcactaca
      721 cgatgggcgg gctgtgggtg gattataacc tgatgtcgaa cctcccggga ttgttcgtgc
      781 tcggcgaggc caacttctcc gatcacggcg cgaatcggct cggcgcgtcg gcgttgatgc
      841 agggtttggc ggacggttac tttgtcattc cttataccgt cggcgattat cttgcgacgc
      901 agaagcccgg ctcgcgtccg tcggccgatg ccgcggagtt caaggcggcg gagcagaatg
      961 taaccggcct gacgtcgcga ctgctgggga tcaacggcaa ggagccggtc agccatttcc
     1021 acaagcggct cggcaagatc atgtgggagg gttgcggcat ggcgcggacg gcggcgacat
     1081 tgacgaaggc gttgcaggag attccaaagc tgcgcgaaga attctgggcg aacgtgaaag
     1141 tgccgggttc ggcggcgacg ttgaaccagt cgctcgagca ggcggggcgg gttgccgact
     1201 ttctggagtt gggcgagttg atgtgtctcg acgcgctgac gcgcgaggag agctgcggcg
     1261 gacatttccg cgaagagttc caatatcccg acggggagtg caaacgcgac gacgagaagt
     1321 tttcgcatgt ggccgcgtgg gaattccaag gcgacggcaa ggcgccgctg cgcaatgtcg
     1381 agccgttgaa ttacgaattc gtgaagccca gtgtgagaag ctacaaataa ccgaaaccaa
     1441 atcctggtag gtggcgcgct cgcgcgccac gttgcgcggc gagcgcgcaa cctaccgaac
     1501 gacgattcct cccatggtcg ctgccacctc ctcaaaaaac atcagcatca ccctgcgcgt
     1561 ttggcggcag gccaatccca gcgccccggg caagttcgtc gactatcccg cgtcgaatct
     1621 gaactcgaac atgtcgttcc tcgagatgct cgacgtggtg aacgacgagc tgacgaagaa
     1681 aggcgaggag ccgatcgcgt ttgcgcacga ttgtcgcgag ggaatttgtg gcacgtgctc
     1741 gctgacgatc aatggcaagc cgcacgggcc ggggcagggc atcgcgacgt gtcagaccta
     1801 catgcgaagt ttccacgatg gcgacgtgat cgtcgtcgag ccgtttcgtg cgcgggcgtt
     1861 tccgttggtg aaggatctcg tggtggatcg cagtgcgttc gacaagattc agcaggcggg
     1921 tggtttcatc agcgtgcgca cgggtgcggc gccggatgcg aattcgattc cgattccgaa
     1981 gggcgacgcg gatctcgcga tggatgcggc ggcgtgcatc cagtgcggcg cgtgcgtggc
     2041 ggcgtgcaag aacgcgagcg cgatgctgtt cgtggccgcg aaggtgtcgc acctcggttt
     2101 gttgccgcag ggccagccgg agcgcgatcg ccgcgtgctg gcgatggtga acacgatgga
     2161 cgaactcggt ttcggcagtt gcacgaatca at
//
LOCUS       DASPLT010001138         2747 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_136901, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001138 DASPLT010000000
VERSION     DASPLT010001138.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 2747)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2747)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2747
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_136901"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..890
                     /locus_tag="VEA63_11695"
     CDS             <1..890
                     /locus_tag="VEA63_11695"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HYD84714.1"
                     /translation="LRLGVPFAIGTLLLAPLAYYPAYLQLAAEDAPHTFWRQWLALGA
                     WPPGPLWFLPVLLAFDALAVLVLRFFPFWNEKPAALGSLTKTRPLSAYLTLLILAIAA
                     YLPMSFVADPFGWWVWGPFVVQGSRWALYAVYFFFGVALSVGKVGGDLFTLTAKLGHR
                     WKAWFAAAVFMFAAFALVLVAAFYADQQNSEHAFLVGVAARIAYAATGVTTSFAMLAF
                     FTRSDAPHRPLFARLVPNAFAMYIVHHGIVTWLQYALLGTPLSATTKGIAVVFAAIVL
                     SWTTAAALRKIPALERVLR"
     gene            966..1397
                     /locus_tag="VEA63_11700"
     CDS             966..1397
                     /locus_tag="VEA63_11700"
                     /inference="COORDINATES: protein motif:HMM:NF025683.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4383 domain-containing protein"
                     /protein_id="HYD84715.1"
                     /translation="MRKRPSAIQWLAWFYCANFILIVTLSHWPGLTDIEGRLLGRFTI
                     DPIDDIFHLLSGLLAGWAAWKSHQWSVNYFRYAGIPYAIDAITGIFFGVEFLNGEICR
                     AGPGDADLTMQNVLVNLPHILIPFTMMLIGFWLAKRVRVPA"
     gene            1415..2527
                     /locus_tag="VEA63_11705"
     CDS             1415..2527
                     /locus_tag="VEA63_11705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD84716.1"
                     /translation="MTRPRWRCCAYAVLIGGASIAIAVAAFLFIASRAQPNFKPVAQP
                     LAPEIASRIDRLPHYRRAEDATYLTFVEWYLVFNPQEYAQFIAANRPSRFPYFRGIGQ
                     IWSSYSEVYGIARQHYPFNVGYHLMLVVIGVSSTAEFAIKGVYETTIGRVFEWTASGE
                     ATPEDIYAANVAHNYGAFITTQPWFEFPFGEKLRGLWSEPTFFGTHFLRKCERKFFLS
                     LEYGVKALYAATIRFASRSVYGVADTEVYLSAHNVGAATLSQPGVRGVEELDERYWIL
                     AVPHYQGFTDAIPLIARDGVDFDEIAGNDEIMLTLIAPAAWTYDLSGGRPLFTMPLLV
                     NVSFKRVAVQVPVKSLGSVLREIGAKDLKLEHLFDY"
     gene            2535..>2747
                     /locus_tag="VEA63_11710"
     CDS             2535..>2747
                     /locus_tag="VEA63_11710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD84717.1"
                     /translation="MKKFARIALILAGLALAVVVGRPVLFLANVWWHDEPAPRSMPAA
                     GTGDASRLYENQPSEVIDLALDPEKAE"
BASE COUNT          471 a          903 c          752 g          621 t
ORIGIN      
        1 tgcttcgcct cggcgttcct ttcgccatcg gcacgctgct cctcgcgccg ctcgcctact
       61 accctgccta ccttcaactc gccgccgaag acgccccgca tactttctgg cgccaatggc
      121 tcgcgctcgg tgcgtggccg ccgggtccac tctggtttct cccggtgttg ctcgctttcg
      181 atgcgctggc ggtcctcgtg ctccgcttct ttcccttttg gaatgagaaa cccgcggcgc
      241 tcggttcgct gacaaagacg cgtccgctat ccgcttacct cacacttctg atcctcgcga
      301 tcgccgccta tctgcccatg agcttcgtgg ccgacccgtt cggctggtgg gtgtggggac
      361 cgttcgttgt gcaaggctcg aggtgggcgt tgtatgccgt ttatttcttt ttcggcgtcg
      421 cgctcagcgt cggcaaagtg ggtggcgatc tgttcacgct gacggcaaaa ctcggccatc
      481 gctggaaagc ctggttcgcc gccgccgtgt tcatgtttgc cgcgttcgcg ctcgtgctcg
      541 tcgccgcatt ctacgctgac caacaaaaca gcgagcacgc gttcctcgtc ggtgtcgcgg
      601 cgcgcatcgc ttacgccgca accggcgtga cgacgagctt cgcgatgctc gcgttcttca
      661 cgcgctccga cgcaccccac cgcccgcttt tcgcccgcct cgttccaaac gccttcgcga
      721 tgtatatcgt ccaccacggc atcgtcacct ggctccagta tgcgctgctc ggcacccctc
      781 tctccgccac aacaaaaggg atcgccgtcg tcttcgccgc catcgttctc tcctggacca
      841 ccgccgccgc gctgcggaaa attcccgcgt tggaacgcgt gctgcgatag tcgcgcaagc
      901 gatgaaagat cgacacattc accgcgtcgg atatgcaaga cggtccagtc ccagcgccaa
      961 caattatgcg gaaacgccca agtgcgattc agtggctcgc gtggttctac tgcgccaact
     1021 ttattctcat cgtgacgcta agtcactggc ccggtctcac ggatatcgaa gggcgtctgc
     1081 tgggccggtt caccatcgat ccaatcgatg acatctttca cctgctctcg gggctgctcg
     1141 ccgggtgggc cgcatggaaa tcgcaccaat ggtcggttaa ctactttcgc tacgccggca
     1201 ttccttatgc aatcgacgcc atcaccggga ttttcttcgg agtcgaattt ctgaacggcg
     1261 aaatttgtcg cgctggtccc ggcgacgccg acctgactat gcaaaatgtc ttggtgaatc
     1321 tgccgcatat actaattccg ttcacgatga tgctgatcgg attttggttg gcaaaacgag
     1381 tgcgcgtgcc cgcctgatgc gaatccgtct ttcgatgact cggccgcggt ggcgttgctg
     1441 cgcttatgcc gttcttatcg gcggtgcttc aattgcgatc gccgtcgcgg cgtttctctt
     1501 catcgcgtca cgcgcccaac ccaatttcaa acccgtcgca caaccgctcg cgccggaaat
     1561 cgcgtcgcgc atcgatcggc tgccccacta tcgtcgcgcc gaggacgcca cttacctgac
     1621 ctttgtcgaa tggtatctcg tcttcaatcc gcaagagtat gcgcaattca tcgcggccaa
     1681 tcgcccgagt cgctttccct actttcgcgg tatcggtcag atctggagta gttatagtga
     1741 agtctacggc atcgctcgcc aacactatcc cttcaacgtc ggctaccatc tcatgctcgt
     1801 cgtcatcggc gttagctcga ccgccgagtt tgcgatcaaa ggcgtctacg aaacaaccat
     1861 cggccgtgtc ttcgagtgga ctgcttccgg cgaagccacg ccggaggata tctacgcagc
     1921 caacgtcgct cacaactacg gcgcgttcat cacgacccag ccgtggttcg agtttccgtt
     1981 cggtgaaaag ctccgcgggc tctggtcgga gcccacgttt ttcggaacgc acttcctccg
     2041 caaatgcgag cgcaagtttt tcctcagcct cgagtatggc gtgaaggcgc tctacgccgc
     2101 gacgattcga ttcgcgagcc gctccgtgta tggcgtcgca gacaccgagg tttacctttc
     2161 cgcgcacaac gtcggcgctg cgacgctttc ccagcccggc gtgcggggcg tcgaggaact
     2221 ggacgaaaga tactggatcc tggcggtgcc gcattatcaa ggcttcaccg acgccatacc
     2281 actgatcgcg cgcgacggag tcgacttcga cgaaatcgcc gggaacgacg aaatcatgct
     2341 cacgctcatc gcgccggcgg cgtggaccta cgatctgagc ggcggccgac cgctcttcac
     2401 catgcctctt ctggtgaacg tcagtttcaa acgtgtcgcc gtccaagtgc cggtgaagtc
     2461 gctcggttcg gtattgcgcg aaatcggcgc gaaagacctg aagctcgaac acctgttcga
     2521 ctattgaatc gcccgtgaaa aagttcgccc ggatcgcact catcctcgcc ggcctagcgt
     2581 tagcggtcgt ggtcggccgc cctgtgcttt ttctggcaaa cgtctggtgg cacgatgaac
     2641 ctgctccgcg gtcgatgccc gcggcgggca cgggcgatgc cagtcggctt tacgaaaacc
     2701 agccatcgga agttatcgat ctggccctcg atcccgaaaa agcggag
//
LOCUS       DASPLT010001139         1384 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_73722, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001139 DASPLT010000000
VERSION     DASPLT010001139.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1384)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1384)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1384
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_73722"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..222)
                     /locus_tag="VEA63_11715"
     CDS             complement(<1..222)
                     /locus_tag="VEA63_11715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD84718.1"
                     /translation="MARSVRSGMTWTTRLVQPAGPSSTIRLFLAVSALALVSSGVGYS
                     QTTPPPADGVGFIGDTLHPQVSGARFSPTP"
     gene            complement(291..>1384)
                     /locus_tag="VEA63_11720"
     CDS             complement(291..>1384)
                     /locus_tag="VEA63_11720"
                     /inference="COORDINATES: protein motif:HMM:NF013955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IPT/TIG domain-containing protein"
                     /protein_id="HYD84719.1"
                     /translation="PSRGAQGDRVAINGAHFSPVPADNNVSFNGAVAVVQSATENQLV
                     VLVPNLASSGDVTVTIAGKKSNGVRFDFLSVGINNGSFEDGTLRAFTLEGSGRVVESW
                     KRVTPTDRQFMAFLDTMADPRDGMSTLTSDAFEVPEGMQTLLFDYNLVATALLRPAAE
                     VLEFYIVTDSATIKVDDLFANVALDIHSPISGFDRGSGFRTAGVLVETWAGTGERVQV
                     RIVLKGRGALPDHIPGMRRYDHNPMGLGNNPGTGVFLDNFRLSSGYEPALPAPDPATL
                     SIVSNGVAVTLSSAPGAFPADARVYIWEIATGSVHAIDVGEDGEFTLTVPFSEHARSA
                     HFLISYATSADDESGGRMFSPQLKLGVTR"
BASE COUNT          259 a          427 c          462 g          236 t
ORIGIN      
        1 gggtgtgggc gaaaacctcg cgccgctgac ctgcggatga agcgtgtcgc cgatgaaccc
       61 gactccgtcc gctggcggtg gagttgtctg cgagtaaccg accccgctgg aaacgagtgc
      121 gagagccgaa acggcgagga aaagtcggat cgtgctgctt ggccctgcag gctgaacaag
      181 gcgtgtcgtc cacgtcatcc cgctcctcac ggagcgagcc acgccggaca tcgtggcccg
      241 gcaacgctga aacagggata gacgttggat cttcaagagc gtggtcggca tcatcgcgtg
      301 actccgagtt tgagttgcgg gctgaacatg cggccgccgc tttcgtcgtc ggcggacgtc
      361 gcgtaggaaa tcaggaagtg cgcggagcgc gcgtgctcgg agaacggcac cgtcaacgtg
      421 aactcgccat cctcgccgac atcgatggcg tgcacgctac ccgtcgcgat ttcccagatg
      481 taaacgcgcg cgtcagccgg aaatgcgccc ggcgccgatg agagcgtgac ggccacgcca
      541 ttcgatacga tcgagagcgt cgcaggatcg ggagccggca gcgcgggttc gtaaccactc
      601 gagagccgga agttgtcgag gaagacgccc gtgccgggat tgttgccgag ccccatggga
      661 ttgtggtcgt agcggcgcat gcccgggatg tggtcgggca gcgctcccct tcccttcagg
      721 acgatgcgca cctgcacgcg ctcgccggtg cccgcccagg tttccaccaa cacgccggcc
      781 gtgcgaaaac cgctgccgcg atcgaaaccg gagatcggcg aatgaatatc cagcgcgacg
      841 ttggcgaaca ggtcgtcgac tttgatcgtc gcggagtcgg tcacgatgta gaactccaac
      901 acctcggcgg ccggccggag cagcgcggtc gcgaccaggt tgtagtcgaa caacagcgtt
      961 tgcatgcctt ccggcacttc gaacgcatcg gatgtcagcg tgctcatgcc gtcgcgcggg
     1021 tccgccatcg tgtcgaggaa ggccatgaac tgccgatccg tcggcgtgac acgcttccac
     1081 gactccacaa cgcgcccgct gccttcgagc gtgaaggccc gcaaggtccc gtcctcgaag
     1141 gaaccgttgt tgattccgac ggagaggaaa tcgaagcgga cgccattcga tttcttgcca
     1201 gcgatcgtga cggtgacgtc gccggaggaa gctaggttcg gcacgagcac gacgagctgg
     1261 ttttccgtcg cgctctggac gaccgcaacg gcgccgttga agcttacatt attgtctgcg
     1321 ggaaccgggc tgaagtgggc gccgttgatc gcgacgcgat cgccttgcgc accgcggctg
     1381 ggca
//
LOCUS       DASPLT010001140         1021 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_114817, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001140 DASPLT010000000
VERSION     DASPLT010001140.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1021)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1021)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1021
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_114817"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..715
                     /locus_tag="VEA63_11725"
     CDS             <1..715
                     /locus_tag="VEA63_11725"
                     /inference="COORDINATES: protein motif:HMM:NF024969.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HYD84720.1"
                     /translation="DTIAAGGLVHVYANGHSRVAVEELVIRMGALTGFHPILQTGLTT
                     FTDVVGPNGIRLCQSIEKIEGLGEKLLQEFDVAPGEPLLAISATGQTQAAVDIAAAWL
                     KRYPENPLIVLCSVAQSDQGTPKHSCGKNLTHLARAAKRGFILDNGMPLGDTSVTLEG
                     KTGTYSVCPLSSIGAVSLVQCLNELTIRELDRRGIAHHVLRNMHLNDTRDTYDAWIRD
                     QRTRYARALNNPAATKPAT"
BASE COUNT          210 a          359 c          282 g          170 t
ORIGIN      
        1 cgacacgatt gccgccggcg gtctcgtgca cgtctacgcc aacggccact cgcgcgtcgc
       61 cgtcgaggag ctcgtcattc gcatgggagc gctcaccggc tttcatccga ttctccaaac
      121 cgggctcacg acattcaccg acgttgttgg gccaaacggc attcgcctct gccagtcgat
      181 cgagaagatc gaagggctcg gtgaaaaact cctccaggaa ttcgacgtcg cgcccggtga
      241 gccgctgctc gcgatttccg ccacgggcca gacccaagcc gccgtcgaca tcgccgcggc
      301 ctggctgaaa cgttatccgg aaaatccgct gatcgtgctg tgctccgtcg cgcagtccga
      361 ccaaggcacg ccgaagcaca gctgcggaaa aaatctcacg catctcgccc gcgccgcaaa
      421 gcgcggcttc atcctcgata atggcatgcc gctcggcgac acatcggtca cactcgaggg
      481 gaagaccggc acgtattccg tttgcccgct gtcttcgatc ggcgccgtga gccttgtgca
      541 atgcctcaac gagctcacga tccgcgaact cgatcgccga ggcatcgcgc accacgtgct
      601 gcgaaacatg cacctcaacg acacgcgcga cacctacgac gcgtggattc gcgaccagcg
      661 cacacgctac gcccgcgcgc tgaacaaccc tgcggccacg aaacccgcca cctgagaaac
      721 ctttcagaag gagaaaccgc gaaacacacc aaacacacga aaactgaaaa tacatcctgg
      781 aatccggacg cccgcgcatg cgcgacgacc cgtgggctac ggcagatcaa acgaatctgt
      841 agccgcaagg tagggcccga cctccgggcg ggccgctcgg agatcggccc ctacctcgaa
      901 cgcgccgcta cagtttcccc ggaagtgctc tggttttctt cgaaccgctc ctggcgtcgc
      961 gtggccgagc gctttcgcga gtcgcgcgct tgagcttcgg ccgtatccgg ctttttacag
     1021 t
//
LOCUS       DASPLT010001141         1328 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_14228, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001141 DASPLT010000000
VERSION     DASPLT010001141.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1328)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1328)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1328
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_14228"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..237
                     /locus_tag="VEA63_11730"
     CDS             <1..237
                     /locus_tag="VEA63_11730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD84721.1"
                     /translation="ANFDRFRHTFNKGDHGYDPAFPSMHGILIANGPAFKAAGGTIDR
                     VENIHVYNLLCAALGLTPAPNDGDDRLVRAMLRP"
     gene            371..613
                     /locus_tag="VEA63_11735"
     CDS             371..613
                     /locus_tag="VEA63_11735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD84722.1"
                     /translation="MKLASLPSRISAATTAVALTVALFATNHTVTVAFFAVAAVGFVG
                     VIAARDYAPRARRWQPSVVTANRPQPRRAHRFRLAA"
     gene            706..>1328
                     /locus_tag="VEA63_11740"
     CDS             706..>1328
                     /locus_tag="VEA63_11740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIM24 family protein"
                     /protein_id="HYD84723.1"
                     /translation="MNPMHVIDYKIHGDDLQFVEIELDPSEAVVAEAGGMMFMDDGIA
                     METIFGDGSQQNSGFMGALLGAGKRLLTGESLFMTVFLNQAPGKKRVAFGAPYPGKIV
                     PVHLADLGGELIAQKDSFLCAAKGVSVGIAFQKRLGTGLFGGEGFIMQRLTGDGLTFV
                     HAGGMIEAIDLKPGEQLRVDTGCLVALQPSVSYDIQFVGKIKTALFGG"
BASE COUNT          231 a          477 c          372 g          248 t
ORIGIN      
        1 gccaacttcg atcgctttcg ccacaccttc aacaaaggcg accacggcta cgatccggcg
       61 tttccctcga tgcacggcat tctgatcgcg aacggcccgg cgttcaaagc cgccggcggc
      121 acaatcgatc gcgtggagaa catccacgtc tacaatctgc tctgcgctgc gctcggcctc
      181 acgcccgcgc cgaacgacgg cgacgaccgc ctcgttcgcg cgatgctccg accgtagcgg
      241 gctcgttcgc gcacaactca cgcctgctgc ggccgtcgcg aaaaaaatct tcgcgcgcgc
      301 aacccgctgt aactttctcc cgcgagcccg tgttcttggc gcatccagta aatctcgaac
      361 acctgtccca atgaaactcg cctcccttcc ttcccgtatc agcgccgcca cgacggccgt
      421 cgcgctcact gtggcgctct tcgccaccaa tcacactgtc accgtcgcct tcttcgccgt
      481 cgccgccgtg gggttcgtcg gcgtgatcgc cgcccgcgat tacgcgccgc gcgcccgccg
      541 ctggcagccg tcggtcgtca ccgccaaccg cccgcagccg cgtcgcgctc accgttttcg
      601 cctcgcagcg tgatccgcgt ccgcaattcc gctcttcacg aaacgctgcc gcatttgaaa
      661 agcggcagcg ttgattttta atctcacgcc cgcttcttca cttccatgaa cccgatgcac
      721 gttatcgatt acaaaatcca cggcgacgac ctgcagttcg tcgaaatcga actcgatccg
      781 tctgaggccg tcgtcgcgga agccggcggc atgatgttca tggacgacgg catcgcgatg
      841 gagacgatct tcggcgacgg ctcgcagcaa aactccggct tcatgggcgc gctgctcggc
      901 gccggcaaac gcctgctcac cggcgagtcg ctcttcatga cggtgttcct gaaccaggcc
      961 cccggcaaaa aacgcgtcgc gttcggcgcg ccgtatccgg ggaaaatcgt cccggtgcat
     1021 ctcgccgacc tcggcgggga attgatcgcg caaaaagatt ctttcctctg cgcagcgaaa
     1081 ggcgtgagcg tcggcatcgc gtttcaaaaa cgactcggca ccggtctctt cggcggcgag
     1141 ggcttcatca tgcagcggct caccggcgac ggcctcacgt tcgtgcacgc cggcggcatg
     1201 atcgaggcga tcgatctgaa gccgggcgag cagctgcggg tcgacaccgg ctgcctcgtc
     1261 gccctgcagc cgtcggtaag ctacgacatc cagttcgtcg gcaagatcaa gacggcgctc
     1321 ttcggcgg
//
LOCUS       DASPLT010001142         1190 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_259398, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001142 DASPLT010000000
VERSION     DASPLT010001142.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1190)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1190)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1190
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_259398"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..550)
                     /locus_tag="VEA63_11745"
     CDS             complement(<1..550)
                     /locus_tag="VEA63_11745"
                     /inference="COORDINATES: protein motif:HMM:NF014724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ElyC/SanA/YdcF family protein"
                     /protein_id="HYD84724.1"
                     /translation="MGFALKKFVSFWIMPLPLCLAVLAVGYGLLLTKRRTRAGRALVG
                     IALVLLLLFSNRTVSKWLVRPLEEPFRPVPELRAGDTPPAELARCRYIVVLGGGHADV
                     PMFSAVNQLSTSALSRLAEGVRLTRALPGTMLVVCGRGAPGRPSHAAVLADAAVSLGV
                     DRERIILLDEPRDTEEEAAALAT"
     gene            705..>1190
                     /locus_tag="VEA63_11750"
     CDS             705..>1190
                     /locus_tag="VEA63_11750"
                     /inference="COORDINATES: protein motif:HMM:NF044415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD84725.1"
                     /translation="MSTVALLRRAPPFEIEFNRASTAFCAVGEQPALHLPIPWLGGSA
                     VERVFAGAEPAGENRGFALSDSGGLLIGCAVQPVGSSIQCATRELYRRLFDVVRPRTL
                     YRIWNYVPAINAVANGVENYRAFCAGRAEVFEATHGAEFKRHLPAASAVGCGGDALAL
                     VF"
BASE COUNT          205 a          377 c          419 g          189 t
ORIGIN      
        1 gcgtggcgag cgcggcggct tcttcctcgg tgtcgcgggg ctcgtcgagc aggatgatcc
       61 gctcgcgatc gacgccgagt gacaccgcgg cgtccgccag caccgccgcg tggctcggcc
      121 ggccgggcgc accccggccg cagacgacga gcatggtgcc gggtaacgcg cgcgtgagcc
      181 gcacaccttc ggccaaccgg ctgagggcgg acgtgctgag ttggttgacg gcggaaaaca
      241 tcggcacatc cgcatgtccc ccgccgagca caacgatgta gcggcagcga gccagctctg
      301 cgggcggagt gtcgccggcg cgcagttcgg ggacgggcct aaacggttcc tccagcggcc
      361 gaacgagcca cttgctcacc gtgcgattgc tgaagagcag gagcagcacg agggcgatac
      421 ccaccagcgc gcgaccggcg cgtgtgcggc gtttcgtgag gaggaggcca taaccgaccg
      481 cgagcaccgc aaggcacagc ggcaacggca taatccagaa ggaaacgaat ttcttcagcg
      541 cgaaacccac cgggagattg gaaacgtgaa cgcgtccgac ggtgcaagat cggcgtggcc
      601 tccgaacgca ctcagtggcg catcgagttt tcggatgcgc gtgtcagggg aacaagttcg
      661 ctcgaggaag gacgatggcg acacgccgtt ccccgggtct ccgcatgtct accgtagctc
      721 ttttacgacg cgcgcctccg ttcgaaatcg agtttaatcg cgccagcacc gcgttctgcg
      781 cggtgggaga gcagccggcg cttcatcttc cgatcccgtg gctcggcggc tcggcggtcg
      841 agcgggtgtt tgcgggcgcc gagccggcgg gcgaaaatcg gggattcgcg ttgagcgact
      901 ccggtgggct cctgatcggt tgcgccgtgc agcccgtggg cagctcaatc cagtgcgcga
      961 cgcgtgagct ctaccgacga ctgttcgacg tcgtgcgacc gcggacgctc taccgcatct
     1021 ggaactacgt gcctgcgatc aatgcggtcg cgaacggagt ggaaaactat cgcgcgttct
     1081 gcgccggacg cgccgaggtg ttcgaagcga ctcatggtgc ggagttcaag cggcacctcc
     1141 cggccgcatc agcggtgggc tgcggaggag acgcgcttgc gctcgtcttc
//
LOCUS       DASPLT010001143         1815 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_217742, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001143 DASPLT010000000
VERSION     DASPLT010001143.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1815)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1815)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1815
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_217742"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1717)
                     /locus_tag="VEA63_11755"
     CDS             complement(<1..1717)
                     /locus_tag="VEA63_11755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HYD84726.1"
                     /translation="MSDYVELHASSAFSFLRGASLPENMAEEAARLELRGLALLDRDG
                     VYGAPRLYGAVREGGVRPIVGAEITMEDGSALPLLVMNQNGYRNLSRLISTAKLVERT
                     GAVVAEWQAALKTAGALNGRLGEASLPDPRERKRPCYATWRELEEHADGLMALTGDEE
                     GPVLRAWARGGAKETEAAMARLEGIFGGDRLAVEVQRRRIRGETTTVNMLADLADARE
                     LPLVATGGVRYATRGERPVADVFTCLRNHTTLDAAGRLLAPNGERHLRGAADMRELFR
                     DLPEAVANSGRLAERLEFTLENLGYQFPDFPVGAGETMAGMLRARVQEGARRKFGTVP
                     KKISDQIETELALIEELKFCGYFLIIWDICEWARQKGILIQGRGSAANSVVCFALGIT
                     AVNPTRYDLLFDRFLSRGRIGKGGHPSWPDVDLDLPSGDLREKVIQEVYQRYAPRGAA
                     MVANVITYRGRSTMREVGKVLGFSEDVLDRFSALYGNGDFPHTLDVEKQVEMAGLPVT
                     HPRLRAMLGLYHQIKGLPRHLGQHSGGMVLCPNHLDTVVPIEPASMEQRCVVQWDKDD
                     CEELGL"
BASE COUNT          333 a          769 c          382 g          331 t
ORIGIN      
        1 ccagccccag ctcttcgcag tcatccttgt cccactgcac cacgcagcgc tgctccatcg
       61 acgccggctc gatcggcacc accgtatcca gatgattggg acatagcacc attccgcccg
      121 aatgctgccc gagatgccgc ggcagcccct tgatctggtg atacagcccc agcatcgcgc
      181 gcagccgcgg atgcgtcacc ggcagacccg ccatctcgac ttgtttttca acatcgagcg
      241 tgtgcggaaa atcgccgttc ccatacaacg ccgaaaaccg atccagcacg tcctccgaaa
      301 atcccaacac cttccccacc tcgcgcatcg tgcttcgccc gcgatacgtg atcacattcg
      361 ccaccatcgc cgccccgcgc ggcgcgtagc gttgatacac ctcctgaatc accttttcgc
      421 gcaaatcccc gctcggcaaa tccaagtcca catccggcca cgacggatgc ccgcccttcc
      481 cgatccgccc ccggctcaaa aatcggtcga agagcaaatc ataccgcgtc ggattcaccg
      541 ctgtgatccc caacgcaaaa cacaccacgc tgttcgccgc cgacccgcgc ccctggatca
      601 aaatcccctt ctgccgcgcc cactcgcaaa tatcccagat gatcaaaaaa tagccgcaga
      661 acttcagttc ctcgatcaac gccagctccg tctcgatctg atccgaaatc tttttcggca
      721 ccgtcccgaa cttccgccgc gcgccttcct gcacgcgcgc tcgcaacatc cccgccatcg
      781 tctcgcccgc gcccaccgga aaatccggaa actgataacc gagattctcc agcgtgaact
      841 ccagccgctc tgccagccgt ccgctgttcg ccaccgcttc cggcagatcg cgaaacaact
      901 cgcgcatatc cgccgccccg cgcaaatgcc gctccccatt cggcgccagc agccgccccg
      961 ccgcatcgag cgtcgtgtga tttctcaaac acgtgaacac atccgccacc ggtcgctcgc
     1021 cccgcgtcgc ataacgcacg ccccccgtcg ccaccaacgg cagctctcgc gcgtccgcca
     1081 gatccgccag catgttcacc gttgtagttt caccgcgaat acgccgccgc tgcacttcca
     1141 ccgccagccg gtctccgccg aaaattccct ccagtcgcgc catcgccgcc tccgtttcct
     1201 tcgcgccgcc gcgcgcccac gcccgcaaca ccggtccttc ctcgtcgccc gtcagcgcca
     1261 tcaacccgtc cgcatgctct tccaactccc gccatgtcgc gtaacacggc cgcttccgct
     1321 cccgcggatc cggtagggac gcctctccga ggcgtccgtt cagcgctccc gctgtcttca
     1381 acgccgcctg ccactccgcc accaccgccc ccgttcgctc caccaatttt gccgtgctga
     1441 tcaaccggct caaattccga tagccgttct gattcatcac cagcaacggc agcgcgctcc
     1501 catcttccat cgtgatctcc gcccccacga tcggtcgcac cccgccttcg cgcaccgcac
     1561 catacaatcg cggcgcccca taaaccccgt cgcgatccaa caacgccagc ccgcgtaatt
     1621 ccaaccgcgc cgcctcctcc gccatatttt ccggcagcga cgccccgcgc aaaaaactaa
     1681 acgcactgct cgcgtgcaac tcgacgtaat cgctcatcgc tcagcctctt tctctttcct
     1741 ctttatcttt ctctttcgtc agccacgcag tggccgctgc gccctacctc gcacgcccgc
     1801 tccccactcg ttctc
//
LOCUS       DASPLT010001144         1346 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_53211, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001144 DASPLT010000000
VERSION     DASPLT010001144.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1346)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1346)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1346
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_53211"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..554)
                     /locus_tag="VEA63_11760"
     CDS             complement(<1..554)
                     /locus_tag="VEA63_11760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoB"
                     /protein_id="HYD84727.1"
                     /translation="MTHPSHSRFIAPLAASAILAFFAGCASQGVKNPSGVGVTEMRAD
                     ERGFVAGTGVESQDLVSVTDKMVRSILGIPEISRAPTPPRIVLDPVVNDTRFPMNKDI
                     FNDRIRIELNRHSMGRVRFLARDRMSTLERERELKQTGQVTASADPNVTEFRGADYFL
                     TGKLSGMTTKTSAGTSDYVLYSFQL"
     gene            complement(582..1259)
                     /locus_tag="VEA63_11765"
     CDS             complement(582..1259)
                     /locus_tag="VEA63_11765"
                     /inference="COORDINATES: protein motif:HMM:NF024833.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YMGG-like glycine zipper-containing protein"
                     /protein_id="HYD84728.1"
                     /translation="MKMLGLLLFGLLAAVPVYAGREPHARGHGHGRIGEGHRHRISHT
                     GRHPGHHYGRGHWHHRPHVHRHWPRYSSAWSYGYGWPYSRAYYAYPYGSYTSLGVGFY
                     DDDFSYRANRGNYAANGLLLGALAGAVIGNNSGDLHNNAWLGAGLGAATGYIAGSVAE
                     NRARRREAVEAANNVGVGHVYASQAQSQQAATPAVETVSTSTASSATATSARTSNMTA
                     ANRLFGR"
BASE COUNT          261 a          431 c          412 g          242 t
ORIGIN      
        1 agttgaaagc tgtagagcac ataatcgctc gtgcccgcgc tcgtcttcgt ggtcatgccg
       61 gaaagttttc cggtgagaaa ataatccgcg ccgcgaaact ccgtcacgtt cggatccgcg
      121 ctcgccgtca cctgccctgt ctgcttcagc tcgcgctcgc gctccagcgt gctcatgcga
      181 tcgcgcgcga ggaaacgcac gcggcccatc gagtgacggt tcagctcgat gcggatgcga
      241 tcgttaaaga tgtccttgtt catcgggaac cgcgtgtcgt tcaccaccgg atcgagcacg
      301 attcgcggcg gcgttggcgc gcgggaaatt tccggaatgc ccagaatgct gcgcaccatc
      361 ttgtcggtga cgctcacgag atcctgggac tcgacgcccg tgccggcgac gaaaccgcgc
      421 tcgtcggcgc gcatctcagt gacgccgacg ccggaaggat ttttaacgcc ctgcgaggcg
      481 cagccggcga agaacgcgag gatcgcgctc gcagccaaag gagcaatgaa gcgggaatgg
      541 gagggatggg tcatggtaaa gagggtgaac gagcgccgcg ttcaacgacc gaacaggcga
      601 ttcgccgcgg tcatgttgga cgtgcgtgcg gacgtggctg tggctgaaga cgccgtgctc
      661 gtcgacacgg tttcaaccgc tggcgtcgca gcttgctggc tctgcgcctg cgatgcgtaa
      721 acgtgtccaa cgccaacgtt gttcgccgcc tccaccgctt cgcgccgccg cgcgcggttc
      781 tccgcgacgg aaccggcaat gtagccagtc gccgcgccca aacccgcgcc gagccacgcg
      841 ttgttgtgca gatcgccgct gttgttgccg atcaccgcgc ccgcgagcgc gccaagcagc
      901 agcccgttcg ccgcataatt cccgcgattc gctcggtagg aaaaatcgtc gtcgtaaaaa
      961 ccgactccta acgatgtgta actgccgtaa ggatacgcgt aatacgcccg agaatacggc
     1021 cagccgtaac catacgacca agcgctggaa taccttggcc aatgccggtg aacgtgcgga
     1081 cggtgatgcc agtgtccgcg gccgtaatga tgaccaggat gccgccccgt atgcgaaatc
     1141 cggtgacgat gcccctcgcc gattcgccca tgaccatgac cgcgcgcgtg cggctcgcgc
     1201 ccggcataaa ccggcaccgc ggccagcaag ccgaacaaaa gaagacccag cattttcatc
     1261 gcgcgcattc tttgagggag acatggcgct ctcgtttagg tttcccacgc ctccggaact
     1321 gtcggcggct ccgcgagatc gatctt
//
LOCUS       DASPLT010001145         1312 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_259422, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001145 DASPLT010000000
VERSION     DASPLT010001145.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1312)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1312)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1312
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_259422"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..814)
                     /locus_tag="VEA63_11770"
     CDS             complement(<1..814)
                     /locus_tag="VEA63_11770"
                     /inference="COORDINATES: protein motif:HMM:NF024742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HYD84729.1"
                     /translation="MESSASSGRPSNVTAPADKVPVPQKIVYGLGTCHDMWGHWLYPN
                     VGWQVFNIFLGVAPWLIGVALFLNRLFDALSDAIFGWLSDNTRTRWGRRRPYILVAGV
                     LAGLGLPLLVAVAPGWGSTMVFGTEVPHYFWFMVISSAVYLPIMSAFKMPFDCLGYEM
                     TPDYHERTSVWSFRNAVQKLPELGLFFAAQFMTMSVWVGASGSDWFDRVTLLFTDGNA
                     WKPAAEGTRPNMLLAAQVYTVVLGLIMAAAAVVMFSVLRERYYGKVTERKQAK"
     gene            complement(965..>1312)
                     /locus_tag="VEA63_11775"
     CDS             complement(965..>1312)
                     /locus_tag="VEA63_11775"
                     /inference="COORDINATES: protein motif:HMM:NF036482.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 protein"
                     /protein_id="HYD84730.1"
                     /translation="SRDTIYVRIALEIGDERVNEETVFLTSPRFLNLPEAKTRATVRL
                     EGPGRATITFKSNAFQHRFAFDLTGMECWSSDNFFELYPNEEKRVTVRFSGRKTAAQL
                     KQRLTWLSLVDTY"
BASE COUNT          283 a          451 c          350 g          228 t
ORIGIN      
        1 tcttcgcctg ctttcgctcc gtcaccttgc catagtaacg ctcgcgcagc accgagaaca
       61 tcacgaccgc cgccgcggcc atgatcagcc cgagcaccac ggtgtaaacc tgcgccgcga
      121 gcagcatgtt cggccgcgtt ccttccgctg ccggtttcca tgcgttgccg tcggtgaaca
      181 acaacgtcac gcggtcaaac caatcgcttc cgctcgcacc gacccacacc gacatcgtca
      241 tgaactgcgc cgcgaaaaac agccccagct ccggcagctt ctgcaccgca ttgcgaaacg
      301 accacacgct cgtgcgctcg tgataatcgg gcgtcatctc gtagccgaga caatcgaacg
      361 gcattttaaa cgcgctcatg atcggcagat aaaccgccga cgaaatcacc atgaaccaaa
      421 agtagtgcgg cacctccgtg ccgaacacca tcgtcgaacc ccaacccggc gccaccgcca
      481 ccagcagcgg aagacccaac ccggccagca cgccggcgac caggatatac ggccgccgcc
      541 ggccccagcg cgtccgcgtg ttgtccgaca accagccgaa aatcgcatcc gaaagcgcgt
      601 cgaaaagccg gttcaaaaac agcgccacgc caatcagcca cggcgccacg cccaggaaaa
      661 tattaaacac ctgccatcca acatttggat acagccagtg gccccacatg tcgtgacacg
      721 tgccgaggcc atatacgatt ttttgaggca ccggcacctt gtcggccggt gcggtgacgt
      781 tggacggcct gccggaagaa gcggatgatt ccatgtcggg gcgcgggggt tgggcggttg
      841 cagaccgctc aacccaagga atcgtggcaa tggaaaaaac gtcccgaccg tccgacttct
      901 cggcgtctgg tcgtgagtga gcgcctgcgt gcacagtgag cgcgagccgc ttcccggcgc
      961 ccgctcaata cgtgtccacg agcgacagcc aggtgagtcg ttgcttcagc tgcgccgccg
     1021 tttttcgtcc ggagaatctc accgtcacac gcttctcctc gttcggataa agttcgaaga
     1081 agttgtcgct gctccagcac tccatcccgg tcagatcgaa tgcgaaccga tgttgaaatg
     1141 cgttcgattt gaacgtgatc gtcgcacgcc ccggcccctc gagccgcacc gtcgcgcgcg
     1201 tctttgcctc cggcaggttc aggaaacgcg gcgacgtgag aaacaccgtt tcctcgttca
     1261 cccgctcatc gccaatctca agtgcaatcc gcacgtagat ggtatcgcgt ga
//
LOCUS       DASPLT010001146         1325 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_300690, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001146 DASPLT010000000
VERSION     DASPLT010001146.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1325)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1325)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1325
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_300690"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1293
                     /locus_tag="VEA63_11780"
     CDS             <1..1293
                     /locus_tag="VEA63_11780"
                     /inference="COORDINATES: protein motif:HMM:NF019259.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1592 domain-containing protein"
                     /protein_id="HYD84731.1"
                     /translation="ADQQPCATQILTGLARQAFRRPVTDADMTPIMRFYQSGLEQAGF
                     ESGIQKGIMAILGSTKFLYRAEPLPEGAQPGDVFPVTDVELASRLSFFLWSTGPDEEL
                     IALAEQNKLSEPAVLKQQVRRMLAHEKAEALTTNFAFQWLKIGDIDAIDPDPRIFPEF
                     DKDLRNSMKRELALFVDSILRADTSVLDLLGAKHSFIDERLARHYGISDVRGSQFRRV
                     ELADERRHGLLGKGGVLLLTSYPNRTSPVLRGAYILETLIGTPPAAPPPGVEIDLDTR
                     EPGQAVTTIRQRLEEHRAQPSCNQCHGVIDPMGIALENFDAIGLWRDVDRFAAAPIDA
                     QGRTVSCEPLTGPADLREALLARPDQFVWALTEKLMTFALGRTVEHYDMPIVRSVARR
                     AAENDYRFEEIVLGVVESDAFRMKSVPVEEGEGTLSLK"
BASE COUNT          255 a          434 c          403 g          233 t
ORIGIN      
        1 gcggatcagc agccgtgcgc aacgcagatt ctcacggggc tcgcgcgcca ggctttccgc
       61 cgtccagtca cggatgccga catgacgccg atcatgcgtt tctaccagag cggcctcgag
      121 caggccggct tcgagagtgg catccagaaa ggcatcatgg caattctcgg ttcaaccaag
      181 tttctgtatc gcgccgaacc gctgccggaa ggcgcgcagc cgggcgacgt gtttccggtt
      241 accgacgtcg agctcgcgtc gcggctgtcg ttcttcctgt ggagcacggg cccagacgaa
      301 gaattgatcg cgctcgccga gcaaaacaag ctcagcgaac ccgccgtatt gaagcagcag
      361 gtgcgccgca tgctcgcgca tgagaaagca gaagcgctta cgaccaactt tgccttccag
      421 tggctgaaga tcggcgacat cgacgcgatc gatcccgatc cgcgcatctt ccccgaattc
      481 gacaaggacc tgcgcaactc gatgaaacgc gagctcgcct tgttcgtcga cagcatcctg
      541 cgcgccgata cgagcgtgct cgacctgctc ggcgcgaagc acagtttcat cgacgagcgc
      601 ctcgcgcgcc attacggcat cagtgacgtg cgcggttcgc agttccgccg cgtcgaactg
      661 gccgatgaac gccgccacgg tctgctcggc aaaggcggcg tgctgttgtt gacctcgtat
      721 cccaaccgca cgtcgccggt gctgcgcggt gcgtacattc tcgaaacgct gatcggcacg
      781 ccgcccgccg cgccgccgcc cggcgtcgaa atcgatctcg acacacgcga acccggccag
      841 gcggtcacga caatccgcca gcgcctcgag gaacatcgcg cgcagccttc ctgcaaccag
      901 tgccatggcg tgatcgaccc gatgggcatc gcactcgaaa acttcgacgc tattggactg
      961 tggcgcgatg tcgaccgctt cgccgcggca ccgatcgatg cgcagggcag aaccgtcagc
     1021 tgtgaaccat tgaccgggcc cgccgatctg cgcgaagccc tgctcgcgcg ccccgaccag
     1081 ttcgtctggg cactgaccga aaaattgatg acgttcgcgc tcggccgcac tgtcgaacac
     1141 tacgacatgc cgatcgttcg cagcgttgcg cggcgcgcgg cggaaaacga ttaccgcttc
     1201 gaagaaatcg tgctcggcgt tgtcgagagc gatgcgttcc ggatgaagtc ggtgccggtg
     1261 gaagagggtg aaggcacgtt gtctttgaaa tagattcgtc attcccgcct gcgcgggaat
     1321 gacga
//
LOCUS       DASPLT010001147         1804 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_259451, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001147 DASPLT010000000
VERSION     DASPLT010001147.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1804)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1804)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1804
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_259451"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..181
                     /locus_tag="VEA63_11785"
     CDS             <1..181
                     /locus_tag="VEA63_11785"
                     /inference="COORDINATES: protein motif:HMM:NF013103.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase family
                     protein"
                     /protein_id="HYD84732.1"
                     /translation="QPDTEVFYKVSDYYSPEHEGGLIWDDPCVCVDWGIAAEDVVIVE
                     RDRSFPRLADLPAFF"
     gene            191..1255
                     /gene="rfbB"
                     /locus_tag="VEA63_11790"
     CDS             191..1255
                     /gene="rfbB"
                     /locus_tag="VEA63_11790"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000699467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HYD84733.1"
                     /translation="MKALITGGAGFIGSALVRHVIGELGWSAVVLDKLTYAGHLSSLA
                     SVSDAPGFAFIRADVCERGALDAAFAQQQPDVVFHLAAESHVDRSITHAAAFIETNVL
                     GTFHVLESARAYWDGLKGEAKGRFRVLHVSTDEVYGSLGPDGLFSETTPYDPRSPYSA
                     SKASADHLASAWFHTYGLPVLISNCSNNYGPYQLPEKLIPLTILNGLEGRKLPVYGDG
                     SNVRDWLYVDDHVEALCLIVEKGVLGRCYNVGGRNERTNIDVVRGICELLDQYAPKAT
                     AHEKLIELVKDRPGHDHRYAIDASRLEDELGWRAKETFDTGLERTVRWYLDNEAWWAP
                     LRAAGVGVQRLGLAKSAGPA"
     gene            1300..>1804
                     /locus_tag="VEA63_11795"
     CDS             1300..>1804
                     /locus_tag="VEA63_11795"
                     /inference="COORDINATES: protein motif:HMM:TIGR01214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar nucleotide-binding protein"
                     /protein_id="HYD84734.1"
                     /translation="MRRAATRGLAIEALGRPDVDLERPESVRAAIAERRPQVVINAAA
                     YTAVDTAEDETERAFLINAGGAEAIADAAARGGAQLIHVSTDYVFAGYKAAPYLEEDE
                     TGPRSVYGASKLEGERLVRAAHPGAVIVRTAWVFDAAGANFVRTMLRLAKSRAEVSVV
                     GDQLGCPT"
BASE COUNT          325 a          512 c          597 g          370 t
ORIGIN      
        1 ccagcccgat accgaggtgt tctacaaagt gagcgactat tacagtcccg agcacgaggg
       61 cggtctgatc tgggatgatc cttgtgtgtg cgtggattgg ggaatcgcgg ctgaagatgt
      121 tgtgatagtg gagcgtgatc gcagctttcc acggcttgcc gatctacccg catttttcta
      181 acaccagcca atgaaagcgc ttatcacagg cggagcgggc tttatcggat cggcgcttgt
      241 tcgccatgtc attggcgagc tcggttggtc ggccgttgta ctcgataagc tgacttacgc
      301 tggccatctc tcttctctgg cgtcggttag cgatgcgcct ggcttcgctt ttatcagagc
      361 ggatgtctgt gagcgaggcg cgctcgatgc cgcgttcgcg cagcagcagc cggatgttgt
      421 ctttcacttg gcggccgaaa gccatgtcga ccgctccatc acgcatgcgg cggcattcat
      481 cgagacgaat gtcttgggca cgtttcatgt gctcgaatcg gcgcgcgcct attgggatgg
      541 cctaaagggc gaggccaaag gccgcttccg cgtgctgcac gtctcgacgg acgaagtcta
      601 cggctcattg ggcccggacg gactgttcag cgaaaccacg ccttatgatc cgcgctcccc
      661 atactcggcg agcaaggcga gcgcagatca tctcgcaagc gcctggtttc atacgtatgg
      721 cctgcccgtg ctgatctcga attgctccaa taattacggg ccgtaccagc tgccggaaaa
      781 gttgatcccg ttgacgatcc tcaacggctt ggaggggcgc aaacttccgg tctacggcga
      841 tgggagcaac gtgcgcgact ggctttatgt cgacgatcac gtcgaggcgc tctgtctcat
      901 tgtcgagaaa ggcgtcttgg gccgctgcta taatgtcggc ggtcggaatg agcgcacgaa
      961 catcgatgtc gtgcgcggca tctgcgagtt gctcgaccaa tacgcgccga aagcgactgc
     1021 ccatgaaaag ctgatcgagc tggtgaagga tcggcccggt cacgaccacc gctatgcgat
     1081 cgacgcctcg cgcctggaag atgagctcgg ctggcgcgcg aaagaaactt tcgacaccgg
     1141 cttggagcgt acggtgcggt ggtacttgga caatgaagcc tggtgggcgc cgctgcgtgc
     1201 ggcaggcgtt ggcgtgcagc gcctcggact tgctaaaagt gcggggcccg cgtgaaaatt
     1261 ctggtgattg gtcgcggcgg gcaggttgcg cgcgcacttg tgcgccgcgc cgccacgcgc
     1321 gggttggcga ttgaagcgct agggcggcct gatgtcgatc ttgaacggcc ggaaagcgtg
     1381 cgtgcggcga tcgccgagcg ccggccgcaa gtggtcataa acgcggccgc ctacaccgcc
     1441 gtcgacacag ccgaggacga aacggaacgc gcttttttga tcaacgcagg cggcgccgaa
     1501 gccatcgcgg atgcggcggc tcgtggcggc gcgcagttga tccatgtttc gacggattac
     1561 gtgtttgcgg gctacaaggc cgcgccatat cttgaagagg atgagacggg gccacgcagc
     1621 gtctatggcg cctccaaact cgaaggcgag cgtcttgtgc gcgccgctca tccgggtgcg
     1681 gtcattgtgc gcacggcgtg ggtgtttgac gccgctggcg ctaacttcgt gcgcacaatg
     1741 ctgcggctcg cgaagtcgcg cgcagaggtc agcgtagtgg gcgaccagct cggttgcccg
     1801 acat
//
LOCUS       DASPLT010001148         1036 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_94548, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001148 DASPLT010000000
VERSION     DASPLT010001148.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1036)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1036)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1036
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_94548"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..578
                     /locus_tag="VEA63_11800"
     CDS             <1..578
                     /locus_tag="VEA63_11800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012377062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HYD84735.1"
                     /translation="KTGHELLEKTIGIVGLGRIGKEVAIRARAFGMNPIGYDVYWDEK
                     FAAEHGVKRAATLDEIFAASDYLSLHTNLTPETRDLISAKNIAKMKRGVLILNCARGE
                     IVHTADMIEALTSGQVGGYGTDVLDEEPPKPDHPLLKLPNVVCTPHIGSRTYESVVRQ
                     ATAAVKNLILAMNGEKPLAQINPEVATKKVV"
     gene            686..>1036
                     /locus_tag="VEA63_11805"
     CDS             686..>1036
                     /locus_tag="VEA63_11805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007358737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ldh family oxidoreductase"
                     /protein_id="HYD84736.1"
                     /translation="MSEIFYVVPEEQHNALVEAAYKKRGYSSQEAAAGARFCAEATRH
                     GIRTHNALKALHLDHLFGSGSGGCVPKAKIEKVKTRFRGAAVWNANKKLGQATGYAAI
                     EQAIKLADKYGVGMV"
BASE COUNT          225 a          298 c          342 g          171 t
ORIGIN      
        1 gcaaaacggg gcacgaactg ctcgagaaga cgatcggcat cgtgggcctc gggcgcattg
       61 gcaaggaagt cgcgattcgc gcgcgggcgt ttgggatgaa cccgatcggg tatgatgttt
      121 actgggacga gaaatttgcc gcggagcacg gcgtgaagcg cgcagcgacg ctcgacgaga
      181 ttttcgcggc gtcggattat ctgtcgttgc acacgaacct gacgcccgag acgcgcgacc
      241 tgatcagcgc gaagaacatc gcgaagatga agcggggcgt gctgatcttg aactgtgcgc
      301 ggggcgagat cgtgcacacg gcggacatga tcgaggcgtt gacgagcggg caggtgggcg
      361 gctacggcac cgacgtgctc gacgaagaac cgccgaagcc cgatcatccg ttgctcaagc
      421 tgccaaacgt cgtgtgcacg ccgcacattg gctcgcgcac ctatgagagc gttgttcgcc
      481 aggcgacggc tgcggtgaag aacctcatcc tcgcgatgaa cggcgagaag ccgctcgcgc
      541 agatcaaccc cgaggtcgcg acgaagaagg tggtctgagc ggccgcctgt tccactcgtt
      601 ctcgttctct taatcgttct cgttctctca gccgagaaag agaacgagaa cgaagaacga
      661 gaacgataaa accaattttt cccccatgtc tgaaattttc tacgtcgtcc cggaagaaca
      721 gcacaacgcc ctcgtcgaag ccgcgtataa gaagcgcgga tactcctcgc aggaggccgc
      781 ggccggcgcg cggttttgcg cggaggcgac gcgtcacggc attcgcacgc acaacgcgct
      841 gaaggcgctg catctcgatc acctgttcgg ctccggctcg ggcggctgcg tgccgaaggc
      901 gaagatcgag aaggtgaaaa cgcgtttccg cggcgcggcc gtctggaacg ccaacaagaa
      961 gctcgggcag gcgacgggtt acgcggccat cgagcaggcg atcaaactcg ccgacaaata
     1021 cggcgtcggg atggtg
//
LOCUS       DASPLT010001149         1155 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_300718, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001149 DASPLT010000000
VERSION     DASPLT010001149.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1155)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1155)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1155
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_300718"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..249
                     /locus_tag="VEA63_11810"
     CDS             <1..249
                     /locus_tag="VEA63_11810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HYD84737.1"
                     /translation="ETMGMINHRLQLDLMKPRTLGFHLHDVNAQGQDHQPIGTGHIDF
                     KMVSEYWRPEHVLVLELSPRVSVAGVKASKAAVEALLG"
     gene            complement(513..>1155)
                     /locus_tag="VEA63_11815"
     CDS             complement(513..>1155)
                     /locus_tag="VEA63_11815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HYD84738.1"
                     /translation="SISFAAPAACVDGNVVRILARLTADATPFRDSATASKAFAPLAD
                     ALLTHASPGDHNQAMMELGATVCFRQKPLCLTCPVRAFCAAGQRGDPEKYPRLAPKQI
                     EQRAVTRIWCERDGALLLHCATADARRLANQHELPTAEHAGVSTEALRSTDLLATKRR
                     AITRFQITETIYATRAPRGKLAAELAWIPLAQLDTITLSGPHRRWIAEILAKR"
BASE COUNT          203 a          302 c          441 g          209 t
ORIGIN      
        1 gaaacgatgg ggatgatcaa tcaccggctg cagctcgact tgatgaagcc gcgcacgctc
       61 gggtttcatt tgcacgacgt gaacgcgcag gggcaggacc atcagcccat cgggacgggg
      121 cacatcgatt tcaagatggt gagcgagtat tggcggccgg agcacgtgct cgtgctggag
      181 ttgagtccgc gggtgtcggt cgcaggcgtg aaagcatcga aggcggcagt cgaggcgctg
      241 ctggggtaaa ggcgcggagc cggtaggttc tcgccaccaa agccgtttgg cgccacgcag
      301 atcatgtaac gtaagcggtt acaccgcatc ggtgctgtcg ccggattaac cttatacaat
      361 cagatggacg gacggggatg cccgcagtgt gtcgtagcga aacgcgtgag cgtttcgtgc
      421 gtgcgaagac gaaaagctca cgcttttcgc tacggggtga atttttggag agagcggcga
      481 aggcgacggt gcgcgaccgc cccggcctcg acctaccgtt tcgcaaggat ttcggcgatc
      541 caccggcggt gggggccgga gagggtgatc gtgtcgagtt gagcgagggg aatccacgcg
      601 agttcggcgg cgagttttcc gcgtggcgcg cgtgtggcgt agatcgtttc ggtgatttgg
      661 aagcgcgtga tcgcacggcg ttttgtcgcg agcaggtcgg tgctccgtag tgcttcggtg
      721 gagacgccgg cgtgctcggc ggtgggaagc tcgtgctggt tggcaagccg gcgcgcatcg
      781 gcggtggcgc aatggaggag gagggctccg tcgcgttcgc accagatgcg ggtgacggcg
      841 cgctgttcga tctgctttgg cgcgagccgt ggatattttt ccggatcgcc gcgctggccg
      901 gcggcgcaaa acgcgcgcac ggggcaggtg aggcagagcg gtttttgacg gaagcacacg
      961 gtcgcgccga gttccatcat cgcctgattg tgatcgcccg gcgacgcatg cgtgagcaac
     1021 gcgtcggcga gcggggcgaa cgctttcgac gcggtggcgg aatcgcggaa cggcgtggcg
     1081 tcggcggtga gccgcgcgag aatgcgcacg acattgccat cgacgcaggc ggcgggagcg
     1141 gcgaacgaaa tactg
//
LOCUS       DASPLT010001150         1140 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_14338, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001150 DASPLT010000000
VERSION     DASPLT010001150.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1140)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1140)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1140
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_14338"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..901
                     /locus_tag="VEA63_11820"
     CDS             <1..901
                     /locus_tag="VEA63_11820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012377320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carbohydrate-binding family 6 protein"
                     /protein_id="HYD84739.1"
                     /translation="PHPESGNVSIPDYVAAVQRGVAPTGTTPLQVAEQLHRHADRAFA
                     AVDTIGRGDPSDVHSDSAAELRRTLEDIRAMAHLGKYYAHKIRAATELALFRATLDRA
                     HHTTLAAELNQSAYQWRGYASLALTYYQNPVWLNRVGHVDWRDLYTSVLYDLTTTDSP
                     VAVPSIPPTSGGTLLEAEDATTNNAPVRNTTPGFTGAGYRDMAGSQGRRWIEWIFDAP
                     AAGRYILELRYAMRRHETEPAALTLNGEATVPFMIWPTGGMQTWAWDRKIVTLRAGPN
                     AIRLAPPLGPNIDHLNILPLEPR"
     gene            complement(953..>1140)
                     /locus_tag="VEA63_11825"
     CDS             complement(953..>1140)
                     /locus_tag="VEA63_11825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD84740.1"
                     /translation="FSRVAIVPESAGGGETLAVTFSSTTKGHEWTRMKNQEEGVVVDQ
                     SQFDRDPIASDASAWLL"
BASE COUNT          226 a          436 c          288 g          190 t
ORIGIN      
        1 gccccacccg gaaagcggca acgtctcgat tcccgactac gtcgccgccg ttcaacgtgg
       61 cgtcgctccc accggcacca cgccactgca agtcgccgag cagctccacc gccacgccga
      121 ccgcgccttc gccgcggtcg acacgatcgg tcgcggcgat ccttcggacg tccactctga
      181 ttccgccgcc gagctccgtc gcacgctcga agacatccgc gcgatggctc acctcgggaa
      241 atactacgcc cacaaaatcc gcgcggccac cgagctcgct ctcttccgcg ccacgctcga
      301 ccgcgctcat cacacaaccc tcgccgccga gttgaaccaa tccgcgtatc agtggcgcgg
      361 ttacgcgtcg ctcgcgctca cctattacca aaaccccgtt tggttaaacc gcgtcggcca
      421 cgtcgactgg cgcgatctct acacgtccgt gctctacgat ctcacgacga ccgattcgcc
      481 ggtcgccgtc ccgtcgatac cgcccacgtc cggcggcaca ctcctcgaag ccgaagacgc
      541 gaccaccaac aacgcgcccg tgcgcaacac cacacccggt ttcacgggtg ccggctatcg
      601 cgacatggcg ggttcacaag gccgccgctg gatcgaatgg atcttcgatg cccccgccgc
      661 cggccgctac atcctcgaac tccgttacgc gatgcgccgc cacgaaaccg aacccgccgc
      721 gctcacgctc aacggcgaag ccacggtccc attcatgatc tggccaaccg gcggcatgca
      781 aacgtgggca tgggatcgca agatcgtcac gcttcgcgcc ggacccaacg ccatccgcct
      841 cgctccaccg ctcggcccaa acatcgatca cctgaacatt cttccgctcg agccgcgctg
      901 actcggaacc atgcgctgaa gaaccacaaa gggacacgaa ttcacacgaa tgtcagagga
      961 gccatgccga agcatccgag gcgatcggat cacgatcgaa ctgcgattga tctacaacca
     1021 cgccttcctc ctgatttttc attcgtgtcc attcgtgtcc cttcgtggtt gaactgaatg
     1081 ttacggctaa ggtttcgcca ccgccggcgg attccggcac gattgccacg cgagaaaacg
//
LOCUS       DASPLT010001151         1620 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_37553, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001151 DASPLT010000000
VERSION     DASPLT010001151.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1620)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1620)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1620
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_37553"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1086)
                     /locus_tag="VEA63_11830"
     CDS             complement(<1..1086)
                     /locus_tag="VEA63_11830"
                     /inference="COORDINATES: protein motif:HMM:NF019095.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHRD domain-containing protein"
                     /protein_id="HYD84741.1"
                     /translation="MKTTLLRTVLVAGGLSAFASMPLCAQIVEFRATINAAQETPATN
                     SPAIGTAVLLYDVAANTFDLTVEIKDLTNTITASHVHEGAVGVAGPVVTGLGAEAVYV
                     RDGTTLRGTFTGVEHMGTPLTLLQNGAYLNFHSAAFPAGEVRGQLVAQPKRLVAVLRG
                     DQEVPDTGSEAYGAALITYDPGTNKITTRLNIYNFTNTLTNSHYHDGAVGVSGPVVHG
                     LGGAAVYTQNGTFYGAVFKDQTYGGDPVELLKGEAYLNVHSNVNPPGEIRGQVVVSDE
                     LDVARLENLSSRAFVGTGDQVLISGFVIRGTEPVRVLITAKGPSLTAAGVTGVLMNPH
                     LSLHDWESRQVVANDDFATSFSIEDMPS"
     gene            1284..>1620
                     /locus_tag="VEA63_11835"
     CDS             1284..>1620
                     /locus_tag="VEA63_11835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD84742.1"
                     /translation="MAFRSGLVLLLPLLLLSACRDAQVATYRVPAEKPEPLPPVLTGG
                     VPAPSPATTADATMANTAVPTADGETLTWTAPTHWQPKPASAMRKGSYAITGADGAAA
                     DLSITAFPGD"
BASE COUNT          291 a          557 c          490 g          282 t
ORIGIN      
        1 cgacggcatg tcttcaatcg agaacgacgt cgcaaaatcg tcgtttgcca ccacctgccg
       61 cgactcccaa tcgtgcagcg acaggtgcgg attcatgagc acgcccgtga cgcccgccgc
      121 ggtcagcgac ggacctttgg cggtgatgag cacgcgcacg ggctcggtgc cccggatgac
      181 gaaaccgcta attaaaacct gatcgccggt gccgacgaac gcgcgcgacg acaagttctc
      241 cagccgagcg acgtcgagtt cgtcgctcac gaccacctgc ccgcgaatct cgcccggcgg
      301 gttcacgttg ctgtgcacat tgagatacgc ctcgcctttc aacagctcga ccggatcgcc
      361 gccgtaagtc tggtccttga acaccgcgcc gtagaacgtg ccgttttgcg tataaaccgc
      421 cgcgccgccg agcccatgca ccacgggccc gctcacgccc accgcgccat cgtggtaatg
      481 cgaattcgtg agcgtgttgg tgaagttgta gatgttgagc cgcgtcgtga ttttgttcgt
      541 gccgggatcg taagtgatca gcgcagcacc gtaggcctcc gaacccgtgt ccggcacctc
      601 ctgatcgccg cgcaacaccg ccacgagccg cttcggctgc gcgaccaatt gcccgcgcac
      661 ttcacctgcg ggaaacgccg cggagtggaa attcagatac gcgccattct gcaaaagcgt
      721 cagcggcgtc cccatgtgct ccaccccggt gaacgttccg cgcaacgtcg tgccgtcgcg
      781 cacgtaaacc gcctcggcgc caagtcccgt gaccaccggc ccggcgacac ccaccgcgcc
      841 ttcgtggacg tgcgaggcgg tgatcgtgtt cgtgaggtcc ttgatctcca ccgtgagatc
      901 gaaggtgttc gccgcgacgt cgtagagcag cacggcggtg ccgatggccg gcgagttggt
      961 ggcgggggtt tcctgcgcgg cattgatcgt ggctctgaac tccacgatct gcgcgcaaag
     1021 cggcatcgag gcaaaggcgg acaagccgcc tgcaaccaac acggtccgaa gcaaggttgt
     1081 tttcatgggg atgggggacg cggcgacccg ctccacgccg acgccgacag gcgagctcgg
     1141 agcggacaca tcgcgcaccc aatgcaccta ggctgcgcta acttctttcg ctaagaagtt
     1201 atcgtaacaa gaccgacaga tatcccgtgc gcgggcggat tggtttgcag tttcgcgtcc
     1261 gaaagcctga ttcgtgacgc ttcatggcct tccgatccgg tctcgttctg ctccttccgc
     1321 tactgctgct atcggcctgc cgcgacgccc aggtcgccac ttaccgcgtg cccgcggaaa
     1381 aacccgagcc gcttccgccc gtcctcaccg gtggcgtgcc agctccctct cccgcgacga
     1441 ctgctgacgc cacgatggcc aacaccgccg tgccgaccgc cgacggcgaa accctcacgt
     1501 ggacggcgcc gacgcattgg cagccgaagc ctgcgagcgc gatgcgcaag ggcagctatg
     1561 cgatcacagg cgcggatggt gcggcggccg atctctcgat cacagccttc ccgggcgacg
//
LOCUS       DASPLT010001152         2062 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_198735, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001152 DASPLT010000000
VERSION     DASPLT010001152.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 2062)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2062)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2062
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_198735"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(104..538)
                     /locus_tag="VEA63_11840"
     CDS             complement(104..538)
                     /locus_tag="VEA63_11840"
                     /inference="COORDINATES: protein motif:HMM:NF015639.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HYD84743.1"
                     /translation="MQEALRGFQVFDRWQILGAHASKAARILHPSQPQSRRTLDRQPG
                     ACRLSSAVIASHLDIPLCAGCGRCCHLVVELRAGDRVPEELVVEHDGVRCMDQRGDGA
                     CVALDPSTRLCTLYETRPQTCRDFHRGETLCRQILSHAQNPR"
     gene            479..607
                     /locus_tag="VEA63_11845"
     CDS             479..607
                     /locus_tag="VEA63_11845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD84744.1"
                     /translation="MGAKYLPTIKDLKATQRFLQKQKPLPKFKKETGGAAAKRKQK"
     gene            complement(776..>2062)
                     /locus_tag="VEA63_11850"
     CDS             complement(776..>2062)
                     /locus_tag="VEA63_11850"
                     /inference="COORDINATES: protein motif:HMM:NF015585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HYD84745.1"
                     /translation="PHIAKIAELHANDAPTHGSFLMHWLDENDAGAANASWAQPDFDD
                     RTWKFVSVPSSGGFAELGVPVDPAVVWFRREIVLPDPLPAGAAKIFLGVIERMETVYL
                     NGQWTGASSWVENPRIYTIKPEALRPGRNLVALRVFKRKPDGGFLSPPERLRIELGDG
                     TSIPLAGEWKSAVGFDARPPAVLPLDFNNYPTMPTVLYNGMIAPVAPLAIAGALWYQG
                     EANREHAEQYRRLMPALISDWRATFGQGEFPFYIAGLPAFMQRSAKAGDEVDGWTRIR
                     EAQAATVRNVRNTGLAITVDTGDADDIHPRVKQPIGERLALLALAGHYRKDVVARGPS
                     LRFVERRAGALALHFDHADGGLEVRGDTLGEFAIAGADRAWHWAEARIEGDDVVVISS
                     PEVPRPAFARYAWQANPLATLFNDAGLPAEPFRTDE"
BASE COUNT          396 a          632 c          699 g          335 t
ORIGIN      
        1 gaaacggctg cggacgtttt cgctggagcg cgaggcggca aagctcgcgg accaattcgc
       61 gcgagcggcg ggccgctaga ccggccggcc cgagctccgg agcctagcgc gggttttgag
      121 cgtgcgaaag aatctggcgg cagagcgttt cgccgcgatg gaagtcgcgg caggtttgag
      181 gccgcgtttc gtagagcgtg caaagccgag tggagggatc gagtgcgacg cacgcgccat
      241 cgccgcgttg gtccatgcag cgcacgccgt cgtgttcgac cacaagctct tccggcacac
      301 ggtctccggc tctcagctcg accaccagat ggcagcagcg tccgcaaccg gcgcaaaggg
      361 gaatatccaa atgagacgcg ataacggcgg agctgagccg gcaagcgcca ggttggcgat
      421 ctaaagttcg gcgcgattgt ggttgcgacg ggtgaagaat ccgggcggcc ttcgacgcat
      481 gggcgccaag tatctgccaa cgatcaaaga cctgaaagcc acgcagcgct tcctgcaaaa
      541 gcagaagccg ctgcccaagt tcaagaagga aaccggtggc gcggcggcga agcggaagca
      601 gaagtagccg tgccgggctt gcccggccat ggcactgaaa aaaaagcgag gccatggaac
      661 acagaggacc actgagcgct gacacagagg tcgcagagca aggccgagtg ttgggctcgt
      721 tgaacgctgt ggcggcgtcg gtccgatcag cgcgggatct gcgcaccccg caggtttact
      781 cgtcggtgcg aaacggttcg gcgggaaggc cagcgtcgtt gaacaaagta gcaagcggat
      841 tggcctgcca cgcatagcgc gcgaaggcgg gcctcggcac ttcgggcgac gagatcacga
      901 cgacatcgtc gccctcgatc cgcgcctcgg cccaatgcca tgcgcgatcc gcgccggcga
      961 tcgcgaattc gccgagcgtg tcgccgcgca cctccaggcc gccgtcggca tgatcgaagt
     1021 gcaacgcaag cgcgccggcg cgccgttcga cgaaacgcag actcgggccg cgggcgacaa
     1081 cgtctttccg ataatgaccc gcaagtgcga gcaatgccag gcgctcgccg atcggttgct
     1141 tgacgcgcgg gtgaatgtca tcggcgtcgc ccgtgtcgac cgtgatggcg agaccggtat
     1201 tgcggacgtt gcgcaccgtc gccgcctgcg cttcgcggat gcgcgtccag ccgtcgacct
     1261 cgtcgcctgc cttcgcgctg cgttgcatga acgcgggcag gccggcgatg tagaagggaa
     1321 actcgccctg gccgaaggtg gcgcgccagt ctgagatcag cgcgggcatg agcctccgat
     1381 attgttcggc gtgttcgcgg ttggcttcgc cttgatacca aagcgcgccg gcgatcgcga
     1441 gcggtgcgac gggagcgatc atgccgttgt aaagcacggt cggcatggtc ggatagttgt
     1501 taaaatcgag cgggagcacc gcgggcggcc gggcatcgaa gccgaccgcc gatttccatt
     1561 cgccggcgag cgggatggag gtgccgtcgc cgagttcgat ccgcagcctt tcgggcggag
     1621 aaagaaatcc tccgtcgggc tttcttttga acacgcgcag cgcgacgaga tttcgaccgg
     1681 gccgaagcgc ttcgggtttg atcgtgtaga ttcgcggatt ctccacccac gagctcgcgc
     1741 ccgtccactg accgttgaga taaacggttt ccatacgctc gatcacaccg aggaaaattt
     1801 tcgctgctcc cgcgggcaac ggatcgggca acacgatctc gcggcgaaac cacaccaccg
     1861 ccggatccac gggcacgccg agttcggcga atccgccgga cgacggcacc gagacgaatt
     1921 tccaggtgcg gtcgtcaaaa tcgggctgag cccacgacgc gttggccgct ccagcgtcgt
     1981 tttcgtccag ccaatgcatg agaaaactgc cgtgcgtcgg cgcgtcgttc gcgtgcagtt
     2041 cggcgatctt cgcaatgtgc gg
//
LOCUS       DASPLT010001153         1168 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_156856, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001153 DASPLT010000000
VERSION     DASPLT010001153.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1168)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1168)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1168
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_156856"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1168)
                     /locus_tag="VEA63_11855"
     CDS             complement(<1..>1168)
                     /locus_tag="VEA63_11855"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HYD84746.1"
                     /translation="ILLNLAGNAIKFTERGEVIVTIAKIGEADRRVRLKFSVRDTGIG
                     ISPEAQARLFHPFAQADHTIASKFGGTGLGLAISRQLVALMGGEIGVDSKPGEGSTFW
                     FTVELPLQTISPRIVAPRLELAGRHALVVDDNATNRDILCRQLAGWRVKAVATGTAEE
                     ALALLRSPAANAAPFDFCLLDVQLPGMSGLDLARAIRAERAFSNTKIVLLSSIGQTLS
                     RQELSERGISACLVKPVRQMLLQETLEHLAVPPATDASTAKPHVAPPMLPTELHLRIL
                     LAEDNPVNQQVARLQLRKFGYEPEIVSDGERAFEAVTTSRYDVVLMDCQMPVTDGFES
                     TRRIRRWEAERRDRGETFVAVHIIAMTANAMLGDREACLAAGMDDYVTKPVRPT"
BASE COUNT          185 a          328 c          438 g          217 t
ORIGIN      
        1 tcagtggggc ggacgggctt tgtcacgtag tcgtccatgc cggcggccag gcaagcttcg
       61 cggtcgccca gcatggcatt cgccgtcatc gcgatgatgt gcacggccac gaatgtttcg
      121 ccgcggtccc ggcgttcggc ctcccaccgg cgtatccgcc gcgtggattc aaagccgtcg
      181 gtcacgggca tctggcaatc catcaacacg acgtcatagc gcgaggtcgt gacggcttcg
      241 aaggcgcgtt cgccgtcgga cacgatttcc ggctcgtagc cgaatttccg gagctggagt
      301 cgcgcgactt gctggttcac gggattgtct tcggcgagca ggatgcggag gtggagctcc
      361 gtggggagca tcggcggcgc gacgtggggc tttgcggtgc tggcgtcggt cgcgggagga
      421 acggcgaggt gttcaagcgt ttcttgaaga agcatctggc gcacgggttt gacgaggcag
      481 gcgctgatgc cgcgctcgct gagttcctgg cgggagagcg tctggccgat cgagctgagg
      541 aggacgattt tcgtgttcga gaacgcgcgc tcggctcgga tggcgcgcgc gagatcgagg
      601 ccgctcatcc cggggagttg cacatcgaga aggcagaaat cgaagggcgc ggcgttcgcc
      661 gcgggcgatc ggagaagcgc gagcgcttcc tcggcggtgc cggtggcgac ggctttcacg
      721 cgccagccag cgagctgacg gcagaggatg tcgcgattgg tggcgttgtc gtcgacgacg
      781 agcgcatgac ggccggcgag ctccagtcgg ggcgcgacaa tgcgcgggct gatcgtttga
      841 agcggcagct cgacggtgaa ccagaaggtc gagccttcgc cgggtttgct gtcgacgccg
      901 atttcgccgc ccatgagggc aacgagttgc cggctgatcg cgagcccgag ccccgtgccg
      961 ccgaacttgc tggcgatggt gtggtcggct tgggcaaagg ggtggaaaag cctcgcctgc
     1021 gcttcaggcg agatgccgat gccggtgtcg cgcacggaaa atttcaggcg gacgcggcgg
     1081 tcggcttcgc cgattttcgc aatggtgacg atgacctcgc cgcgttcggt gaacttgatc
     1141 gcgttgccgg cgagattgag gagaattt
//
LOCUS       DASPLT010001154         1205 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_14379, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001154 DASPLT010000000
VERSION     DASPLT010001154.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1205)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1205)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1205
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_14379"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..862)
                     /locus_tag="VEA63_11860"
     CDS             complement(<1..862)
                     /locus_tag="VEA63_11860"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HYD84747.1"
                     /translation="MISVITPTTGHPRLKDAILSVQRQLERGFRHVVVIDGVERKART
                     EAILHACNFDGDVVVLPQATGGGGFNGHLIYATMPFLVQTEFLALLDEDNTYAPTHLS
                     SLLALCRRHDLDWAYSLRSVFTDDGVSMDDDCQSLGWWPAFDAPYHLIDTSCYFVRRE
                     IFKQGATIWDRRVNAGEHKGPEFVFCEWLFTQHPRGTTTGEATMNYWLGSSQGRTQVE
                     RMNYFRAGNAINRRIYREFPWRFTTFADTKRGERVRFQCKDVALWKEIQLIVGDAVPL
                     KISSVSAAVLR"
     gene            complement(992..>1205)
                     /locus_tag="VEA63_11865"
     CDS             complement(992..>1205)
                     /locus_tag="VEA63_11865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD84748.1"
                     /translation="RWLSECDVSFALVFTKADKQSAQQTRNKIDLFKQSVSEWRATFP
                     IFFVTSTESKIGRSEILAFVEAQRNA"
BASE COUNT          247 a          410 c          327 g          221 t
ORIGIN      
        1 gacgcagcac cgcggcgctc accgatgaaa ttttcaacgg caccgcatcg ccgacgatca
       61 actgaatttc cttccacaac gcgacgtcct tgcactgaaa ccgcacgcgt tcgccgcgct
      121 tggtgtcggc aaacgtcgtg aaccgccagg gaaactcgcg ataaatccgc cggttgatcg
      181 cgttgcccgc ccgaaaataa ttcatccgct ccacctgcgt ccgcccctgc gacgaaccca
      241 gccagtaatt catcgttgcc tcgcccgtcg tcgtcccgcg cggatgctgc gtgaagagcc
      301 attcgcaaaa cacgaactcc gggcccttgt gctcgcccgc gtttacccgc cggtcccaga
      361 tcgtcgcccc ctgtttgaaa atctcccgcc gcacgaaata gcagctcgtg tcgatcaagt
      421 gatacggcgc atcgaacgcc ggccaccagc ccaggctctg gcaatcgtca tccatcgaga
      481 cgccatcgtc ggtaaacacc gaccgcaacg aatacgccca gtcgagatcg tgccgcctgc
      541 acaacgcgag cagcgacgag agatgcgtcg gcgcatacgt gttgtcctcg tcgagcagag
      601 ccagaaactc cgtctgcacc aaaaacggca tcgtcgcgta aatcagatgc ccgttgaacc
      661 cgccgcctcc cgtcgcctgc ggcagcacga ccacgtcgcc gtcgaagtta cacgcgtgca
      721 ggatcgcttc cgtccgcgcc tttcgctcga cgccatcgat gacgaccacg tgccggaaac
      781 ctctctcgag ctggcgctgc acgctgagaa ttgcatcctt cagtcgcgga tgcccggtcg
      841 tcggagtgat gacagaaatc atgggaaacg gcgtgattgc ggcacagtcg tcccattgcg
      901 ggcgagccaa atgtgagcgc gcgaacagcg cggagattgg cgcactcctg aattcgggcg
      961 ccaggtgatc aacttcacgc gggcgacccg ttcacgcgtt ccgctgcgcc tcgacgaacg
     1021 cgaggatttc cgagcgcccg attttgcttt cggtcgacgt cacgaaaaaa atcggaaacg
     1081 tcgcgcgcca ttcgctcacg ctctgcttga agagatcgat tttgttccgc gtctgctgcg
     1141 ccgattgctt gtcggctttt gtgaacacca gcgcgaacga cacgtcgcac tccgagagcc
     1201 agcga
//
LOCUS       DASPLT010001155         1824 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_300790, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001155 DASPLT010000000
VERSION     DASPLT010001155.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1824)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1824)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1824
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_300790"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            97..396
                     /locus_tag="VEA63_11870"
     CDS             97..396
                     /locus_tag="VEA63_11870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD84749.1"
                     /translation="MNTPDTGLFAVLNFSLTREEDGRPTQRTATCGTFLQVERAFEVA
                     REEAERELRRRRAESPQPATIIELLDTEWGYDVRQNFLVVSRYWVHDRAPSELGV"
     gene            complement(816..>1824)
                     /locus_tag="VEA63_11875"
     CDS             complement(816..>1824)
                     /locus_tag="VEA63_11875"
                     /EC_number="2.6.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012377405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LL-diaminopimelate aminotransferase"
                     /protein_id="HYD84750.1"
                     /translation="GPEQGYAFLREAIVAHEFAPRGCKIDADEVFVSDGSKCDCGNIQ
                     EIFSTDLRLAIPDPVYPVYVDTNVMAGRTGANVDGRYQGITYLDSTPANGYVPAIPSV
                     ATDLIYLCFPNNPTGAVATKEQLAAWVAYAKQHKAIILFDAAYEAYIRDPKIPHSIYE
                     IEGARDVAIEFRSYSKIAGFTGTRCAFTVIPKSLKAWDAAGNEHSLHALWNRRHTTKF
                     NGVSYPIQKAAAAIYTDAGKQQTRALTDFYLGNAALIRNAVAKLGFNCIGGDNAPYIW
                     VNVGRDSWEFFDLLLNRAQVVCTPGAGFGKCGEGHVRISAFNSRENVVTALDRIAAAL
                     K"
BASE COUNT          328 a          488 c          656 g          352 t
ORIGIN      
        1 acccgattcg caaagaagga agttgccgcc cgcactgcgc gccgcgcgcg ttgtcggagg
       61 gcgtggcatg agactcgcta ttttcgaaat aacatcatga acacgcctga taccggattg
      121 ttcgccgtgc tgaatttctc gctcacgcga gaagaagatg gccggccgac gcaacggacg
      181 gccacgtgtg ggacgtttct gcaagtggag cgtgcgttcg aggtggcgcg ggaggaagcc
      241 gagcgggaac tgcggcggcg gcgggcagag tcgccgcaac cggcgacgat catcgagctg
      301 ctcgataccg aatggggcta cgatgtgcgg cagaatttct tggtggtgtc gcgctactgg
      361 gtgcacgacc gcgcgccgtc agagctgggc gtgtgacgcg cgcgcgaacg ccacgcgttc
      421 gcgccgaaag gtggaatccg acgtcctcgt cgggtttctt tttgcggcgg gcgcgggcgc
      481 atcgtggggc tacagcagat taaacaaaaa tgtagccgca aaaatgcgca gaaagcgcaa
      541 agatccgctc cggttttgcg ccttctgtgc ctctttgcgg ctattggttc tgaaaacgct
      601 ccagccggat atttcacgct cgttggaata tgccttcgac gtgttttcgc ggaggtaggg
      661 gcggctctcc gagccgtccg cggatgcctc ggagaggcat ccctacctgc ctcgccgtgg
      721 tcgcgcgggt ggtttaacca ggttagtaga aaaggcggaa aggaaaaagc ccggcgggaa
      781 cgccgggctc cacctgttgt gctgactggg tgcgcttact tcagcgcggc ggcgatgcgg
      841 tcgagggcgg tgacgacgtt ctcgcgcgaa ttgaacgcgc tgatgcgcac gtggccttcg
      901 ccgcacttgc cgaaaccggc gccaggtgtg cagacgactt gagcgcggtt gaggaggaga
      961 tcgaaaaact cccacgagtc gcggccgacg ttgacccaga tgtagggcgc gttgtcgccg
     1021 ccgatgcagt tgaagccgag cttcgcgacg gcgttgcgga tgagggcggc gttgccgaga
     1081 tagaaatcgg tgagcgcgcg cgtctgctgt ttgccggcgt cggtgtagat cgcggcggcg
     1141 gctttctgaa ttggatacga gacgccgttg aacttcgtcg tgtggcggcg attccagagc
     1201 gcgtgaagcg agtgctcgtt gcccgcggcg tcccaggctt tgagggactt cgggatcacg
     1261 gtgaacgcgc agcgggtgcc ggtgaagccg gcgatcttcg agtagctgcg gaactcgatc
     1321 gcgacgtcgc gcgcgccctc gatctcgtag atgctgtgcg ggattttcgg atcgcgaatg
     1381 taagcttcgt aggcggcgtc gaagagaatg atggccttgt gctgtttcgc gtaggcgacc
     1441 caggcggcga gctgttcctt cgtcgcgacg gcgccggtgg gattgttggg gaaacagaga
     1501 taaatcaggt cggtcgcgac ggacggaatc gcgggcacgt agccgttggc aggcgttgaa
     1561 tccagatagg tgataccttg gtagcggccg tcgacgttgg cgccggtgcg gccggccatg
     1621 acgttggtgt cgacataaac tggatacacc ggatcgggga tggcgaggcg gaggtcggtg
     1681 gagaaaattt cctggatgtt gccgcagtcg cacttcgagc cgtcggagac gaagacctcg
     1741 tcggcgtcga tcttgcagcc gcgcggcgcg aattcgtggg cgacgatggc ttcgcggaga
     1801 aacgcgtagc cctgctcggg acca
//
LOCUS       DASPLT010001156         1045 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_178324, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001156 DASPLT010000000
VERSION     DASPLT010001156.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1045)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1045)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1045
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_178324"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(164..763)
                     /locus_tag="VEA63_11880"
     CDS             complement(164..763)
                     /locus_tag="VEA63_11880"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HYD84751.1"
                     /translation="MNDDTPVQTQTTGLITPLDFTTFMRNYQDMVFSTAVRLVGNEAQ
                     AEDISQEVFLKAYERYDTLQASPTAGGWLKTVATNLSLNHLSRYRNRWRFFSEFRRDD
                     SGGEADEPEVEFAAPDTFFSGVDASERRAWVEQALDQLPEHQRVPLVLYHFEDLPYDD
                     IAKRLGVSLAKVKTDILRARAALAQILARSGASHEKFSA"
BASE COUNT          198 a          339 c          312 g          196 t
ORIGIN      
        1 tgtgcgtagg attgcttcca ccacggcagc gacgcgcgcg acgcgatggc ggcgaacacg
       61 cgatcctcga gcgagcgcgg cgcgcggcgg tcgggcagcg aacgcagcgt ctgatggatg
      121 aatttttcga gttcttcggg agagcgtttc atggcgtggg attttacgcg gaaaattttt
      181 cgtgggaggc gccgctgcgc gccaggattt gcgcgagcgc cgcgcgcgcc cgcagaatat
      241 ccgtcttcac cttcgcgagc gagacgccga ggcgtttcgc gatgtcgtcg taaggcaggt
      301 cttcgaaatg atagagcacg agcgggacgc gttgatgctc gggaagttga tccagcgcct
      361 gctcgaccca agcacgccgc tcgctcgcgt ccaccccgga aaaaaacgtg tccggggccg
      421 cgaactcgac ctccggctca tccgcttctc cgccggaatc gtcgcgccga aattcggaga
      481 aaaaccgcca gcgattgcgg taacgcgaca ggtggttgag cgacagattc gtcgcgacgg
      541 ttttcagcca gccgccggcc gtcgggctgg cctgcagcgt gtcgtagcgt tcatacgctt
      601 tcagaaagac ctcctgcgaa atatcctccg cctgcgcctc gttgcccacg agccgaacag
      661 ccgtcgaaaa aaccatgtcc tgatagtttc gcatgaacgt cgtaaaatcg agcggggtga
      721 ttaagcccgt ggtttgggtt tgcactggag tgtcatcgtt catgcccaga acacggccgg
      781 tgccgaaaag tcgcaccgaa gatgcgccgc cccgcgctcg cggcgagccc tattgaacaa
      841 atcgtgcgaa acgcgccaat gctgcgccct tgcaactcct gcaagctcgc cgcctccgct
      901 taatcccgtg gcactccgcc gcaggttctc ctcccgcaga acgcgaaggc cgttaccact
      961 ttcgcgccgc gtcctccacc atctgctgca gcatctcgaa acgcacctcg ccgtaattca
     1021 acgcatagcg ctgccgccct tcgcg
//
LOCUS       DASPLT010001157         1881 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_94634, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001157 DASPLT010000000
VERSION     DASPLT010001157.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1881)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1881)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1881
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_94634"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..497)
                     /locus_tag="VEA63_11885"
     CDS             complement(<1..497)
                     /locus_tag="VEA63_11885"
                     /inference="COORDINATES: protein motif:HMM:NF025851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 95 protein"
                     /protein_id="HYD84752.1"
                     /translation="MNKLAGFLGLLAAGIGLATIGRGGEPQGKTHVLWYDAPASSWEA
                     ALPLGNGRVAAMVFGGTDRERIALNEDTLTSGEPPADLRSVNVTATYPEVRALLADGK
                     PQEAEAIIKAKWLGRNQQSYQPLGDLWLEIEGGGEVTDYRRWLDLETAIAGVSYVREG
                     ARFSR"
     gene            complement(816..1721)
                     /locus_tag="VEA63_11890"
     CDS             complement(816..1721)
                     /locus_tag="VEA63_11890"
                     /inference="COORDINATES: protein motif:HMM:NF014376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HYD84753.1"
                     /translation="MPHSAQALPSFMSKQVAAARRFYFEPPSKQPERLAVVSGGWERS
                     DRNYVITRPGFRYFGIEFVAGGRGTLKMRGREFALSRGVVFAYGPGVQHEITTDAKDR
                     LSKYFVDFTGAGAATAMKAAGIGPGSCQTVAIADDVQSAFEHLLAAGRRGTPTAAKIA
                     ALQGEILLCVITDVRLARGAGSAQSRQTFLRCRNLIEEQFATLRTAEEAAAACGVAPA
                     YLSRLFRRFGKQPAYQFLIRMKMNQAAAILRRQALNVSETAAVLGMDPFHFSRVFKRV
                     HGRSPVSFLNESLEDGAAVSEGELE"
BASE COUNT          388 a          703 c          445 g          345 t
ORIGIN      
        1 tcgcgcgaaa acctcgcgcc ctctcgcaca taagaaacac ccgcaatcgc cgtctccaaa
       61 tcgagccatc gccgataatc cgtgacctcg ccaccgcctt cgatctccag ccacaaatcg
      121 cccaacggtt gataactctg ctgattccgt cccagccact ttgctttgat aatcgcttcc
      181 gcctcctgcg gctttccatc agccaacaac gcccgcacct cggggtaagt cgccgtgaca
      241 ttcaccgacc tgagatccgc cggcggctct cccgacgtca gcgtgtcttc attcagcgcg
      301 atccgctcgc gatcggtccc accaaaaacc atcgccgcca cgcgtccatt ccccaacggc
      361 aacgccgcct cccaactcga cgccggcgca tcataccaaa gcacatgcgt ctttccttgc
      421 ggttccccgc cccggccaat cgtagccaac ccaattccgg ccgctaaaag tccaagaaac
      481 ccagccagtt tgttcatgaa cgtattcaac agggatgaag gtttaccacg cgcgccattt
      541 caggcgtccg ccctccctca gtgtcaatcc ttctctgcgt tgtccccagc aaacagggac
      601 aactcctccg taagatcgca cgtcgggcgc ctcgacctta gttcatcgta gtttccaata
      661 aatgtgaact agtgatatgc caaccgatcc ctgaatgaaa cccatggcgc ggacgcgccg
      721 gtagggatgc ctctccgagg catccgccca gtggcgtaga aatgctgaac gactaaagac
      781 atctgtgaga cagcacgcca cctaccgatc gctcgtcact ccaactcgcc ctcgctcacc
      841 gccgcgccgt cctccaaact ctcgttaagg aacgaaaccg gcgagcgccc gtgcacccgt
      901 ttgaacacac gcgaaaagtg aaacggatcc atgcccagca ccgccgccgt ttcgctcacg
      961 ttcagcgcct gccgccgcag aatcgccgcc gcctggttca tcttcattcg aatcaagaat
     1021 tgataggccg gctgcttccc aaatcgccga aacagccggc tgagatacgc cggggcgacg
     1081 ccacaggccg ccgccgcttc ctcggccgtg cgcagcgtcg cgaactgctc ctcgatcaga
     1141 tttcgacaac gcagaaatgt ttgccgcgac tgcgccgacc ccgcgccgcg agcaaggcgc
     1201 acgtccgtaa tcacacagag caggatttcc ccttggagcg ccgcgatctt cgccgccgtc
     1261 ggcgtccctc gccggcccgc cgccagcaga tgctcgaacg ccgactgaac gtcatcggcg
     1321 attgcgacgg tctgacaact gcccggcccg atacccgccg ccttcatcgc cgtagccgct
     1381 cctgccccgg tgaaatccac gaaatacttt gaaagtcggt ccttcgcgtc cgtcgttatc
     1441 tcgtgctgca cgcccggccc gtaggcgaac accacacctc ggctgagcgc aaactcccgg
     1501 ccgcgcatct tcaacgttcc ccggcccccg gccacgaatt cgattccaaa ataccgaaaa
     1561 cccggtcgcg tgatcacgta gtttcggtcc gaccgttccc agccaccgga aaccaccgcc
     1621 aatcgctccg gttgtttcga cggcggttcg aaataaaagc gccgcgccgc cgccacctgc
     1681 ttcgacatga acgacggcaa agcttgagcg gaatgcggca tcgcttcatc caacccgacg
     1741 cggttcgaaa atcaatcggt caataatcgt catgccgcag gcaagaacgt gcattcaccg
     1801 cgtcgctcgc ttccgctatg ctgcagatcg catgccccgc cctcgcctca atccgctctc
     1861 tgccgctcgt ctctacgccg c
//
LOCUS       DASPLT010001158         1009 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_178334, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001158 DASPLT010000000
VERSION     DASPLT010001158.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1009)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1009)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1009
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_178334"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            18..>1009
                     /locus_tag="VEA63_11895"
     CDS             18..>1009
                     /locus_tag="VEA63_11895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD84754.1"
                     /translation="MRDIDLREIQWSKLRAYRRSMVIVALVLVAVVAGAVGWKYLNQS
                     APPWLVRWKLDRYLKQEAHVRDFAVNFPFPSKAEMKAPPEKPAPTLAKGSRTGKDFEA
                     LRREYFTMKMAALRSERDGGRRPRSDASSAATGGEGATAGTSRLQDIAAMEAAMAPIV
                     DDLWEFQRQWAPDSTTADGSGGALATARMELARNIDQQIQTAGGYEAMYRAIGRELFV
                     SKRLLESRNPAHRRMGVEIAFAAARHALDHAVNGHVAARICEGYIVPNTDLATDTNPR
                     SRFHEERFLAEAAEFNRANHEFRNVARLYERYLEDAPNPQRADWARSQLAMAYE"
BASE COUNT          202 a          298 c          337 g          172 t
ORIGIN      
        1 agaactcttt ccggttgatg agggatattg atctccgcga gatccaatgg agcaagctgc
       61 gcgcttaccg gcggagcatg gtgatcgttg cgctcgtgct ggtggcggtt gtggcgggcg
      121 ccgttggttg gaaatatctg aatcaatcgg cgcctccgtg gctcgtgcgt tggaaactgg
      181 accgttatct gaagcaagaa gcccacgtgc gcgatttcgc ggtgaacttc ccctttcctt
      241 ccaaggcgga gatgaaagcg cctcctgaaa aaccggcgcc caccctcgcg aaaggatcac
      301 ggacgggcaa ggacttcgaa gcgttgcgcc gcgagtattt caccatgaaa atggccgcgc
      361 tgcgctcgga aagagacggg ggccggcggc cgcggagcga tgcgagcagc gccgcgaccg
      421 gcggtgaagg cgcgactgcc ggaacaagca gactgcagga tatcgcggcg atggaggccg
      481 ccatggcgcc gattgtcgac gatctttggg agttccagcg ccagtgggcg cccgactcca
      541 cgaccgcgga cggctctgga ggagcgctgg cgacagcccg catggagctg gcgcggaaca
      601 tcgaccagca gatccagacg gctggcggct acgaagcgat gtatcgcgcc atcggccggg
      661 agttgttcgt gagcaaacgc ctgctcgaaa gccggaatcc ggcgcatcgc cggatgggag
      721 tggaaatcgc gtttgccgcg gcgcggcacg ccttggatca cgcagtcaat ggccacgtgg
      781 ccgcgcgcat ttgtgaggga tacatcgtgc cgaacacgga tctcgccacc gatacgaacc
      841 cccgctcccg atttcatgaa gagcgtttcc tggcggaagc ggccgagttc aatcgcgcca
      901 atcacgaatt ccggaatgtc gcgcgcctct acgagcgtta tctcgaagac gctcccaacc
      961 cgcagcgcgc tgactgggcg aggtcacagc tggccatggc ttacgagaa
//
LOCUS       DASPLT010001159         1941 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_300838, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001159 DASPLT010000000
VERSION     DASPLT010001159.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1941)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1941)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1941
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_300838"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..459)
                     /locus_tag="VEA63_11900"
     CDS             complement(<1..459)
                     /locus_tag="VEA63_11900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009509443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HYD84755.1"
                     /translation="MDPVRPTQKDVARTAGVTQATVSLALRNHPEVSLATRTRIQSIA
                     ARLGYAPDPYLAGLSAYRKTRRPAKFQASLAWLSNYPKGESWKNFPAFRGYFEGATQR
                     ARELGYRLEEFRLHTPGMTCARMENILAARNIGGLLVAPQPLSGTTMAFRF"
     gene            653..1804
                     /locus_tag="VEA63_11905"
     CDS             653..1804
                     /locus_tag="VEA63_11905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007359075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD84756.1"
                     /translation="MPQAVTRGNGHYFFGYYDKSPWDASGRWLLGMESTFMDRPPTAD
                     DVVVIGLIDVANNHAWKPLAETRAWNWQQGCMLQWLDGGRSKQIIFNDRRDGRFISRI
                     LNVASGRERVIDRPVYAVNRQGSHAISLNFSRLAHQRPGYGYAGVPDPWLAEQVPEDD
                     GLYAIDLGAGTSRMILSVADAASWRPRPDFAGAVHRFNHAQFSPGGRRFAFLHRWKVP
                     KEEVGTTRLMTLDIDGLDLRCLADDDVVSHYDWRVDGQVLAWARQRDRGDRYFLFNES
                     GAAPGVVGEGVLTCDGHCSVSPDGRWMLTDTYPDATHYRTLILFHWETGERIDIGRFH
                     APPMTWEIRCDLHPRWSPDGRKICLDSIHEGARRMYVLDVSEIVGKADA"
BASE COUNT          341 a          557 c          663 g          380 t
ORIGIN      
        1 gaaacgaaac gccatggtcg tgccggacaa cggctggggc gcgacgagga gtccgccgat
       61 gttgcgcgcc gcgaggatgt tttccatgcg ggcgcaggtc atgcccgggg tgtgaagacg
      121 gaattcctcg agacgatagc cgagttcacg ggcgcgttgc gtggcgcctt cgaagtaacc
      181 gcggaacgcg ggaaaatttt tccagctctc gcccttggga taattcgaaa gccacgcgag
      241 cgacgcttgg aatttcgccg gccgacgggt ttttcgataa gcggacagcc ccgcgagata
      301 agggtcgggc gcgtagccga gccgggctgc gatcgactgg atacgtgtgc gcgtcgccag
      361 cgaaacttcc ggatgattgc gcagcgcgag tgaaaccgtg gcctgcgtaa cgccggcggt
      421 gcgggcgaca tctttctgag tggggcggac gggatccacg gggtgattcg aacggaaaat
      481 cagttggcgg agtggggcga ggcgcggcga gcttataaat ataagggagc gagtctcgtg
      541 gcgcaccgcc actgcttatc agtatgagga gcgggtgcgt tggcggtgtc gggcagattt
      601 ggcgtcgtga ggcatggcag aagcctcagc tcatctttcc accgccggga acgtgccgca
      661 ggccgttacg cgcggaaacg gacattattt ttttggctat tacgacaagt ccccttggga
      721 cgcgtcgggg cgctggctcc ttggcatgga gtcgaccttc atggaccggc ctccgaccgc
      781 ggacgacgtc gtcgtcatcg ggttgatcga tgtcgccaac aaccacgcgt ggaagccgct
      841 ggccgaaacc cgtgcgtgga actggcaaca aggttgcatg ctccagtggc tggatggagg
      901 ccgatcgaag cagatcatct tcaacgatcg ccgcgacgga cgtttcatca gccgcatcct
      961 gaacgtcgcg agtggtcgcg aacgcgtgat cgaccgacct gtttacgcgg tcaatcgtca
     1021 gggctcgcac gcgatttcgc tgaacttctc ccggctcgcg catcaacgtc ctggatacgg
     1081 ttatgccggt gtgcccgatc cgtggctggc ggagcaggtg ccggaagacg acggccttta
     1141 cgccatcgat ctcggcgccg gcacatcgcg aatgattttg agcgtcgccg acgccgcttc
     1201 ctggcgtccg cggccggatt ttgcgggagc cgtgcatcgt tttaatcacg cgcagttcag
     1261 tcccggcgga cgccgcttcg ctttcctgca tcgttggaaa gtgccgaagg aggaggtggg
     1321 gaccacgcgc ttgatgacgc tggatatcga tgggctcgac ctgcgttgcc tcgctgacga
     1381 cgacgtcgtt tcccactatg attggcgggt ggacgggcag gtgctcgcgt gggcccggca
     1441 acgggatcgc ggcgatcgtt actttttatt caatgaaagt ggcgccgctc cgggcgtggt
     1501 cggcgagggt gtgctcacgt gcgacggtca ctgctcggtt tcgccggacg gccgctggat
     1561 gctcaccgac acgtatccgg acgccacgca ttatcgcacg ctgatccttt ttcactggga
     1621 gacgggcgaa cgaatcgaca tcgggcgttt tcacgcgccg ccgatgacgt gggaaattcg
     1681 ctgcgatctt cacccgcgat ggagcccgga cggccgcaag atctgcctcg actccattca
     1741 cgaaggcgcg cggcgcatgt atgtgctcga tgtcagcgaa atcgtcggga aggccgatgc
     1801 ctgaactcgt cctggaaaat cggggaaggc ggcgggcgaa ctccacgtcg cgccccttcg
     1861 cgctttcgtc ggtccgcgcc gatgtgctcg actactgcga gcggttgcgg gatcgcgacg
     1921 gccgctgggg tgcctatcgg a
//
LOCUS       DASPLT010001160         1680 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_137299, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001160 DASPLT010000000
VERSION     DASPLT010001160.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1680)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1680)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1680
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_137299"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..202)
                     /locus_tag="VEA63_11910"
     CDS             complement(<1..202)
                     /locus_tag="VEA63_11910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009508684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CHC2 zinc finger domain-containing protein"
                     /protein_id="HYD84757.1"
                     /translation="MPVIKPSCVRDLKLRVNLADVVSRVVTLRKAGGARLKGLCPFHN
                     EKTPSFNVDPDKGFYKCFGCGKA"
     gene            349..1398
                     /locus_tag="VEA63_11915"
     CDS             349..1398
                     /locus_tag="VEA63_11915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012377378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HYD84758.1"
                     /translation="MRPSLFQLFGLVALAFAGAAQAQPMAAPPPPPAEPAPVWQATLP
                     RFDEATYLRQVEALISSFEKATGQKLAPREKKKVGLKIYTDSGPGMATPIPLVKAVIA
                     ALEQRGFEKENIFLVGLNQLRLRMTGYLPSLVTGKSPFDGHQIYVLESGRYYDPVWFY
                     DSPLPQRFDPIFAEQQTKDFDAETSPDEDRKSFLATPLFLDADFWINLPVYTDHSMLG
                     INGALVNATLWNASNTARFFRSPANAPAAVAEMSAIPELRQTWMFTIASLERFQYVGG
                     PFFNSLYATSEPLLWLSTDPVMMDALMRARIDQLRKRNGFQPISEEIRTLEFAETLGV
                     GSTKIQAAKIIDVGS"
     gene            1498..>1680
                     /locus_tag="VEA63_11920"
     CDS             1498..>1680
                     /locus_tag="VEA63_11920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012377377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HYD84759.1"
                     /translation="MGSAAYFPFLVQVVLAAAITGVVIGASQFFGQRARHTKIKDSAY
                     ECGIPTDTVVHARFSVK"
BASE COUNT          307 a          514 c          518 g          341 t
ORIGIN      
        1 cggctttgcc gcacccgaag cacttgtaga aacccttgtc cggatcgacg ttgaacgacg
       61 gcgttttctc gttgtgaaac gggcacaatc ccttgagccg ggcgccgccg gccttgcgca
      121 acgtgacgac gcgggagacg acatcggcga ggttgacgcg cagcttcagg tcgcgaacgc
      181 aggacggttt gataacaggc atgaacgggc ggtgcgcacg acggcgcgcg gctggagaag
      241 aatgcacttt caacccgcac accccgctgt caagttcccg cgcctccggt gaggatttgc
      301 tcttcccgca ggaaactttc gcgttctccc ggctacttac acatcaccat gcgtccgtct
      361 cttttccagc tcttcggtct tgtcgcgctc gcgttcgcgg gcgcggctca agcccagccg
      421 atggccgcac cgccgccacc accggcggaa cctgcgccgg tgtggcaggc gacgctgccg
      481 cgcttcgacg aagcgactta cctgcggcag gtggaggcgc tcatcagcag cttcgaaaaa
      541 gcgacaggcc aaaaactcgc gccgcgcgaa aagaaaaaag tcggcctgaa aatctacacc
      601 gattccggcc cgggcatggc gacgccgatc ccgctcgtga aggcggtgat cgcggcgctg
      661 gagcagcgcg gtttcgagaa ggagaacatt tttctcgtcg ggctgaatca gttgcggctg
      721 cgtatgaccg gttatctgcc gtcgctggtg accgggaagt cgccgttcga cgggcatcag
      781 atttatgtgc tggaatccgg ccgctattac gacccggtgt ggttctacga ttcgccgctc
      841 ccgcagcgct tcgatccgat ttttgccgag cagcagacga aggatttcga cgccgagaca
      901 tcgcccgacg aagaccgcaa aagttttctg gcgacgccgc tgtttctcga tgccgatttc
      961 tggatcaacc tgccggtcta tacggatcac tcgatgctcg ggatcaacgg tgcgctcgtg
     1021 aatgcgacgt tgtggaacgc gtcgaacacg gcgcgtttct tccgctcgcc ggcgaacgcg
     1081 cccgcagcgg tcgccgagat gagcgcgatt cccgaattgc ggcagacgtg gatgttcacg
     1141 atcgcgagcc tcgagcggtt ccaatatgtc ggcggaccgt tcttcaattc gctctacgcc
     1201 acctcggagc cgctgctgtg gttgagcacc gacccggtga tgatggacgc gttgatgcgc
     1261 gcgcggatcg accaattgcg gaagcgtaat ggcttccagc cgatctcgga ggagatccgc
     1321 acgcttgaat tcgccgagac gctgggtgtg ggttcgacca agattcaggc ggccaagatc
     1381 atcgacgtgg gttcgtgaag tggggtaaat gttcgatagc cggttagggg cggcgcgaac
     1441 gatccggcga acttccctct tgtctccctt agtctgctcc gcccaattca ttccgccatg
     1501 ggttctgccg cgtatttccc gtttcttgtt caagtcgtgc tcgctgccgc gatcacgggc
     1561 gtcgtgatcg gtgccagcca gtttttcggc cagcgcgcgc ggcacacgaa aatcaaggac
     1621 tcggcttacg aatgcggcat cccgacggac accgtcgttc acgcgcgatt ctcggtgaag
//
LOCUS       DASPLT010001161         1961 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_37741, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001161 DASPLT010000000
VERSION     DASPLT010001161.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1961)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1961)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1961
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_37741"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..211
                     /locus_tag="VEA63_11925"
     CDS             <1..211
                     /locus_tag="VEA63_11925"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HYD84760.1"
                     /translation="KTGYAPWQYVMQARLARARRQLSGSDATLEEIAERLGFNSGFHL
                     SRSFKKAFGLSPGRWRRQFRKPRPS"
     gene            313..1740
                     /locus_tag="VEA63_11930"
     CDS             313..1740
                     /locus_tag="VEA63_11930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007361566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose isomerase"
                     /protein_id="HYD84761.1"
                     /translation="MATQPLRIGLFGIGLDAYWPQFTGLKERLENYLGQVARKLARPG
                     VEVVNIGLVDHPARAFAAGRELRRHDVDLIVLHVTTYALSSTVLPVVLRAKVPVLVLN
                     FAPAGAIDYDAFNRMGDRTRMTGEWLAHCSACPVPEIANVFRRAGIPFHQITGVIDED
                     PTAWAEIDAWLDAGKVMAGMHGNRLGLMGHYYSGMLDIYSDLTLHCATFGTHVEMIEV
                     EELVAERRRVTSAAVQARRREFDDAFDIQPDCSTDDLEEAARTSLALDRLVERNELGS
                     FAYYHRGTGVAESETAVASIILGASLLTARGVPVAGEYEVKNAQAMKIMDLFGAGGSF
                     TEYYAMDFQADVVLMGHDGPGHIAIAHGKTKVRPLQVYHGKVGRGVSVEMAVKHGPVT
                     LLSVIETQGKLGLLCAEAESVPGPILEIGNTNSRYRFPGGARGFVNAWNSHGPAHHCA
                     VGVGHIADRIHKLGTLLGLETVQIR"
BASE COUNT          365 a          638 c          597 g          361 t
ORIGIN      
        1 gaagaccggc tacgcgccgt ggcagtatgt gatgcaggcg aggctcgctc gcgcgcgtcg
       61 tcaactttct ggaagcgatg ccacgctcga ggagatcgcc gagcggctcg gcttcaactc
      121 cggcttccat ctctcccgtt cgttcaagaa ggccttcgga ctgtcgcccg gccgctggcg
      181 ccggcagttt cgaaaaccac ggccctccta gcacgcggcg cagggttcgc ggcacgccgc
      241 cggtcattct gtatatggaa aagatcactg cggacatact catccgatcc gcggtgcagt
      301 aggctcgagg tcatggcgac ccaacccctg cgcatcggac ttttcggcat cggcctcgac
      361 gcttactggc cgcaattcac cggcctcaag gaacgccttg aaaactatct cgggcaagtc
      421 gcgcggaagc tcgcgcggcc cggcgtcgag gtggtcaaca tcggtctcgt cgaccatccg
      481 gcccgggcgt tcgccgccgg ccgggagctg cgccggcacg acgtcgacct gatcgtgctg
      541 cacgtgacga cctacgcgct gtcgtccacg gtcctgccag tggtgcttcg ggcgaaggtc
      601 cccgttctcg tcctcaactt cgcgcctgca ggcgcgatcg actacgacgc cttcaaccgg
      661 atgggcgacc gcacgcgaat gacgggcgaa tggctcgcgc actgctccgc gtgtccggtg
      721 ccggaaatcg cgaacgtttt ccggcgcgcc gggattccgt ttcatcaaat cacgggcgtg
      781 atcgacgagg atcccaccgc ttgggcggag atcgatgcct ggctcgacgc cggcaaagtc
      841 atggccggaa tgcacggcaa tcgcctgggc ctcatgggcc actactacag cggcatgctc
      901 gatatttatt ccgacctcac cctgcactgc gcgacgttcg gcacgcatgt ggaaatgatc
      961 gaggtcgaag aactggtcgc cgagcggagg cgcgtcacct ccgccgcagt gcaggcgcgc
     1021 cgccgggagt tcgatgacgc cttcgacatt caaccggatt gctccaccga cgatctcgag
     1081 gaggccgctc gcacctcgct cgcactggat cggctcgttg agcgcaacga actcggctcg
     1141 ttcgcgtatt accaccgcgg caccggcgtc gcggagagcg agaccgccgt cgcgtcgatt
     1201 attttaggcg cctcgctgct cacggcacgc ggcgtgccgg tggccggcga atacgaagtg
     1261 aaaaacgccc aggcgatgaa gatcatggat ctgttcggcg ccggaggatc gttcaccgaa
     1321 tattacgcca tggatttcca agcggacgtg gtgctgatgg gccacgatgg cccgggccat
     1381 atcgcgatcg cccatggcaa gaccaaggtt cgaccgctcc aggtttatca cggcaaagtc
     1441 ggccgcggcg tttcggtcga aatggctgtg aagcacggtc ccgtgacgct gctctcggtc
     1501 atcgagaccc aaggcaaact cggcctgctc tgcgccgaag ccgaatcggt tccaggcccc
     1561 atcctcgaaa tcggcaacac caacagccgc taccgctttc cgggcggagc gcgcggcttc
     1621 gtcaacgcgt ggaattccca cggtcccgcg catcactgcg cggtgggcgt cggccatatc
     1681 gccgaccgta tccataaact gggcacactc ctcgggctcg aaacggtgca gatccgctga
     1741 gcctgaacaa ctcatttgaa ccacgaatgg acacgaatcg acacgaatga aatcacgctc
     1801 gaatacggag ccttatcgga acaccgaagg gtgacgtaac gatcagcctc agcgctatcc
     1861 ttcggctcct ctgaattcgt gttcattcgt gtcgattcgt gatttctctt ctgcaggagt
     1921 ccggctgagt ggacgggatt cgcgcaacgc ggatcagagg c
//
LOCUS       DASPLT010001162         1329 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_178486, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001162 DASPLT010000000
VERSION     DASPLT010001162.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1329)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1329)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1329
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_178486"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..485)
                     /locus_tag="VEA63_11935"
     CDS             complement(<1..485)
                     /locus_tag="VEA63_11935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LapA family protein"
                     /protein_id="HYD84762.1"
                     /translation="MIFAFILGAIIGATALLLPVYRARRAIRRGEEEKQQLAMERQLV
                     VDFMHHMVEALGENAARGDLQQRIVHAAILCSGALSACLFERTEDGQMRSVAVEGLFP
                     PHRPLAPAVRQGLATRARFIEQVLKSETFPDNEGIVGAVVQTGRGQLIADAERDSRVV
                     R"
     gene            complement(577..1083)
                     /locus_tag="VEA63_11940"
     CDS             complement(577..1083)
                     /locus_tag="VEA63_11940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HYD84763.1"
                     /translation="MPETANSVYLVDAHSDPVVVRITGRACFQNSACLRDFVTEMLRQ
                     GKSRFVLDFQHCSSMDSTFLGVLAGIALELRKRPGGGSLVLSRMAPRNLELVRNLGLH
                     RLLTVDAGDFPMKFQGDAALECKDQSELDHARLVLEAHENLVTADESNRSKFQDVLAF
                     LKNRVSQS"
BASE COUNT          266 a          474 c          354 g          235 t
ORIGIN      
        1 tgccgcacca cgcgcgaatc tctctccgca tcggcgatca attgtccccg gcccgtctgc
       61 acgaccgcgc ccacgatgcc ttcgttgtcg ggaaaggttt ccgacttcag cacctgttcg
      121 atgaaccgcg ctcgcgtcgc gagcccctgg cgcaccgcgg gcgcaagcgg ccggtgcggc
      181 ggaaacaacc cctcgaccgc cacactgcgc atctgtccgt cctccgtgcg ctcgaacaaa
      241 cacgcgctca acgcccccga gcacaggatc gccgcgtgca cgatccgctg ctgcaaatcc
      301 ccgcgcgcgg cgttttcgcc cagcgcttcc accatgtggt gcatgaaatc caccaccagt
      361 tgccgctcca tcgcgagttg ctgcttctcc tcctcgccgc ggcggatcgc ccgccgggcc
      421 cgatagaccg gcaacagcaa cgccgtcgct ccaatgatag cgccaagaat gaaagcgaag
      481 atcattacgc gcatcttgat gcgacgaact tttccccgca actcgtcgga tcgaacagga
      541 ccgcgcccgc ccgccgatct cactgcgacg tctcgctcaa ctctggctca cccgattctt
      601 cagaaacgcc agcacatctt gaaatttcga gcggttcgac tcgtccgccg tcaccagatt
      661 ttcatgcgcc tcgagcacga gccgcgcatg atccagctcg ctctggtctt tgcattcgag
      721 cgccgcgtcg ccctgaaact tcattggaaa atcgcccgcg tcgaccgtca gcaaacgatg
      781 cagcccgaga ttgcgcacga gttcgagatt gcgcggcgcc atccgcgaca gcaccaggct
      841 cccgccgccc ggacgtttcc gtaactccag cgcgatgccc gcgagcacgc ccaaaaacgt
      901 gctgtccatg ctcgagcaat gttgaaaatc caacacgaag cgtgacttcc cctgccgcag
      961 catctccgtc acgaaatccc gcagacacgc gctgttctga aaacacgcgc gccccgtgat
     1021 tcgcaccacg accgggtcgg agtgcgcatc gacgaggtaa acgctgttgg cagtttcggg
     1081 catcggttag agcgaaattt tctcagggat ctgaatcgtg ggaaagaaaa gttcgtcggt
     1141 ctcgcccgcc ccgcaagtcg ccgcgttatc cgaccgcatc gaagaatccg acggcggccc
     1201 ggctcaacga ctcggaggaa gcgtcgcgca ggtcaattcc gcgccacgat ttgccgcaac
     1261 acatacggca aaatgccgcc atgctggaag tagtcgattt cgatcggcgt atcgatgcgg
     1321 caccgcacg
//
LOCUS       DASPLT010001163         1561 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_259603, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001163 DASPLT010000000
VERSION     DASPLT010001163.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1561)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1561)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1561
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_259603"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..608)
                     /locus_tag="VEA63_11945"
     CDS             complement(<1..608)
                     /locus_tag="VEA63_11945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD84764.1"
                     /translation="MASPHRIGAAAKPGTRNWSVRFSSASATRELVDRKEARPALSPP
                     LDQAYWRAPQGSTGGLQYLSWGARHFGASPVAVSRPGGWSYVVVEAGSPTLVWGGRRE
                     QIRGPALVLIGPDCGLSWRDVAEQENKHLVWVWRHPVHTVMARLQRDAVAHYALEAAD
                     VSELRHLHVLSRNEIFRGDLHSEAALTGLQSLLEARIARIGEG"
     gene            813..>1561
                     /locus_tag="VEA63_11950"
     CDS             813..>1561
                     /locus_tag="VEA63_11950"
                     /EC_number="3.1.1.99"
                     /inference="COORDINATES: protein motif:HMM:NF020039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HYD84765.1"
                     /translation="MTSSIQVALRVKASLGESPLWDARANRLRWVDIVAKSVGEFDPS
                     TGLNRSVSLETPVSAVAPTHEGALVVAVQEGIARLDPVTGQLSNLWTPPGHDASTMRF
                     NDGKCDTAGRFIAGTMSLTGAGGAASLYSFSPDGSMRTLLRGVSLSNGLDWNAEGTRM
                     YYVDTPTRQIAVFDYDLASGDLLNRRVAVEIPASLGYPDGLTVDVEGMLWLALWGGGA
                     LTRWDPRTGRLLARHSLPVSQVTSCAFGGANL"
BASE COUNT          275 a          539 c          454 g          293 t
ORIGIN      
        1 ccttcgccga tgcgggcaat ccgcgcttcg agcagcgact gcaagcccgt cagcgccgct
       61 tccgaatgca gatctccccg aaaaatctcg ttccggctca acacgtgcag atggcgcagc
      121 tcactcacgt cagccgcctc cagcgcgtaa tgcgccaccg cgtcgcgttg cagccgtgcc
      181 atcaccgtat ggacaggatg ccgccacacc cacacgagat gtttgttctc ctgttccgcg
      241 acatcccgcc agctcaagcc acagtcgggg ccgatcaata cgagcgccgg cccccgaatc
      301 tgctcacgac ggccgcccca gaccagggtg ggcgatccgg cttcaaccac gacatagctc
      361 caaccgcccg gccgcgacac cgcaaccggc gacgctccaa aatgacgcgc gccccacgag
      421 agatattgca acccgccggt cgatccttga ggcgcccgcc aatacgcctg atccaacggc
      481 ggactcagcg cgggccgcgc ttcttttcga tccaccaact cccgcgtcgc ggacgccgag
      541 gaaaaccgca cgctccagtt cctcgtgccc ggcttcgcgg ccgcgccgat tcggtgggga
      601 gaagccaagg attttccgga tgtggcgagg gacattgggg gagaaagttc gttccgactc
      661 cagctggcag tccgtttcac aatgcgaact atatgtttcg tcaaacaaaa taattgccgt
      721 gaacggccga ggcgcgtttt cctccatgtg agcctcgctc gcgaacattg aaattcgcgg
      781 tcgtcgccgt ttccggggct ctgcactttc ccatgacttc atcgatccag gtcgctcttc
      841 gcgtcaaggc ctctctcggg gaaagcccgc tttgggatgc gagggcaaac cggctgcgtt
      901 gggtggacat cgtcgcaaaa tccgtcggcg aattcgatcc atccaccgga ctgaatcgat
      961 cagtgtcgct tgaaacgcca gtgagcgccg ttgctccaac gcacgagggc gctctcgtgg
     1021 tggcggtcca ggaaggcatc gcccggctcg atccggtcac cggtcagctt tcgaatttat
     1081 ggacgccgcc tggccacgat gcgtcgacga tgcgtttcaa cgacggcaaa tgcgacacgg
     1141 cgggccgttt catcgccggc acgatgtccc tcaccggcgc cggcggagca gcgtcgctct
     1201 attccttttc acccgacggc tcgatgcgaa cgcttttgcg cggcgtgtcg ttgtcgaacg
     1261 gcctggactg gaacgcggag ggcacgcgta tgtattatgt cgatacgccc acgcgccaga
     1321 ttgccgtatt cgactacgat ctcgcgtccg gtgacttgct caaccggcgc gtcgcagtcg
     1381 agattcctgc ttcgctcggc taccccgacg gcctcaccgt cgacgtcgaa ggcatgctct
     1441 ggctcgcgct ctggggcggc ggcgcgctca cccgctggga tccgcgcacc ggccggctgc
     1501 tcgcgcgcca ttcgctgccg gtctcgcaag tcacgtcctg cgcctttggc ggagcaaacc
     1561 t
//
LOCUS       DASPLT010001164         1572 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_218076, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001164 DASPLT010000000
VERSION     DASPLT010001164.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1572)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1572)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1572
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_218076"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(68..673)
                     /gene="leuD"
                     /locus_tag="VEA63_11955"
     CDS             complement(68..673)
                     /gene="leuD"
                     /locus_tag="VEA63_11955"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HYD84766.1"
                     /translation="MALEKIIQVVGRAVSVPGNDIDTDRIIPARFMKCVTFDGLGEFL
                     FYDVRKNADGTDKAHPLNDPRFKNATILLSGSNFGCGSSREHAPQAIQKYGFKAVIAE
                     NFAEIFFGNSTGLGIPCVSAAREDIAKIAAAVEKDPTTEVIIDLVKQEVRFGGAAVKV
                     SIRESARDALVNGRWDPIGELLDGLPAVKETAGGLAYFATA"
     gene            complement(776..>1572)
                     /locus_tag="VEA63_11960"
     CDS             complement(776..>1572)
                     /locus_tag="VEA63_11960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HYD84767.1"
                     /translation="DRFTMEERMTVCNMSIEGGARVGYVNPDETTFKYLEGRPYSPKG
                     EAWTAAVARWKATASDAGCHYDDVVTINAADIAPTVTWGINPGQGISINENIPNPDTT
                     TSVDEKAGIIEALAYMKLQPGAPIKGTKIDVAFLGSCTNGRLSDFQEVARFVKGKRVA
                     PGVKAIAVPGSQIVALQCEKLGLDKIFSDAGFEWRAAGCSMCLAMNPDKLIGDQLCAS
                     SSNRNFKGRQGSPTGRTILMSPLMVAAAAVTGSVADAREVFDVNSN"
BASE COUNT          303 a          556 c          396 g          317 t
ORIGIN      
        1 gcggcaagcg cgccatctgc cccatccatc aagcaggcac acccgaaggc ctgccgctac
       61 cgcgtcctca agccgtcgcg aaatacgcca gcccaccggc cgtctctttc accgccggca
      121 aaccgtcgag cagttcgccg atgggatccc agcgaccgtt gaccaaagca tcgcgcgccg
      181 actcgcggat cgaaaccttc acggccgcgc cgccgaaacg cacctcttgc ttcaccaggt
      241 caatgatcac ttccgtcgtc ggatcttttt caaccgccgc cgcgatcttc gcaatgtcct
      301 cgcgtgccgc cgacacgcac ggaatcccga ggcccgtgct gttgccgaag aagatctccg
      361 cgaagttctc cgcgatcacc gccttgaacc cgtatttctg gatcgcctgc ggcgcatgct
      421 cgcgcgatga accgcagccg aagttcgaac ccgacagcag gatcgtcgcg ttcttgaaac
      481 gcggatcgtt cagcggatgc gccttgtccg ttccatccgc gttcttgcgg acgtcgtaaa
      541 acaaaaattc gccgagccca tcgaacgtca cgcacttcat gaaacgcgcc gggatgatgc
      601 gatcggtgtc gatatcgttg cccggcacgc tcaccgcgcg accgaccacc tggataattt
      661 tttcaagagc catggaaatt tattttctga attgtttgtt acattggtcc catcagtccc
      721 atccgtccca taggtcccat aaaataacta cgcgacccat gaccgactca ccgccttagt
      781 tcgaattcac gtcgaacact tcacgtgcat ccgccacgct gcccgtcacc gccgcggcgg
      841 ccaccatcag cgggctcatc aaaatcgtcc gtcccgtcgg cgaaccctga cggcctttga
      901 agttgcggtt cgacgaactc gcgcagagct ggtcgccgat gagtttgtcc ggattcatcg
      961 ccagacacat cgagcaaccc gccgcgcgcc actcgaagcc agcgtcgctg aaaatcttgt
     1021 cgaggcctag cttctcgcat tgcaacgcca cgatctgcga acccggcacc gcgatcgcct
     1081 tcacgcccgg cgccacacgt tttcctttca cgaaacgcgc gacctcttgg aaatcgctca
     1141 agcggccgtt cgtgcacgaa ccgagaaacg ccacgtcgat ctttgtgcct ttgattggcg
     1201 cacccggctg cagcttcatg taggcgagcg cctcgatgat gcccgccttc tcgtcgaccg
     1261 acgtcgttgt atccggattt gggatattct cgttgatcga gataccctgc cccggattga
     1321 tgccccacgt gaccgtcggc gcaatgtccg ccgcattgat cgtgacaaca tcgtcgtaat
     1381 ggcagcccgc atcgctcgcc gtcgccttcc agcgcgcgac cgccgccgtc cacgcctcgc
     1441 ccttcggcga atacggccgc ccttcgagat acttgaacgt cgtctcgtcg ggattcacgt
     1501 agcccacgcg agcgccgcct tcgatcgaca tgttacacac cgtcatgcgc tcctccatcg
     1561 tgaagcgatc ga
//
LOCUS       DASPLT010001165         1304 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_137408, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001165 DASPLT010000000
VERSION     DASPLT010001165.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1304)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1304)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1304
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_137408"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..957
                     /gene="pstA"
                     /locus_tag="VEA63_11965"
     CDS             <1..957
                     /gene="pstA"
                     /locus_tag="VEA63_11965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007360026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HYD84768.1"
                     /translation="GLIVIGDIFLKGSRTVFQSTFPYINVPFLTEAPESLYVFEHEGK
                     TMELGDRDFRAFRAEQEAKARAEGQPVPQFQTQSYVYSAGGIWPCIAGTLLLVVGSMT
                     IALLLGVSSAIYLNEYALDGPVVRFIRLAILNLAGVPSIVFGLFGFGLFVIYLDWNVS
                     LLAGWFTLAFMVLPVVITASEESLKSVPKGFREGSLALGATKWQTIRKNVLPYALPGI
                     LTSSILGIARVAGETAPIMFTAAYVVRDKLPWDVERFSDFFFQGVMALPYHIYVVSSK
                     IPQNEYTARVQYGTAFVFLALVALIALTSIVLRNKLRNRYKW"
     gene            1008..>1304
                     /locus_tag="VEA63_11970"
     CDS             1008..>1304
                     /locus_tag="VEA63_11970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HYD84769.1"
                     /translation="MPSPAVNTAPAVQRSRPGTLSAPGEKPIIEIDRVNFSYGSQRAL
                     NGISLNIEAKKVTAFIGPSGCGKSTLLRCLNRMNDLIEGARVTDGAIRIRGVDIN"
BASE COUNT          233 a          406 c          386 g          279 t
ORIGIN      
        1 gggctgatcg tgatcggcga cattttccta aaagggtcgc gcacggtttt ccaatcgacg
       61 tttccctaca tcaacgtgcc gtttctcacg gaggcgccgg agtcgctcta cgtgttcgaa
      121 cacgagggga aaacgatgga actgggcgat cgcgattttc gcgcgttccg ggcggaacag
      181 gaagcgaagg cgcgcgccga aggacagccg gtgccgcagt ttcagacgca gagttacgtt
      241 tactccgcgg gtggcatctg gccgtgcatc gccggcacgc ttctgctggt cgtcggatcg
      301 atgacgatcg cgctgctgct cggcgtgagc agcgcgatct atctcaacga atacgcgctc
      361 gacggaccgg tcgtgcgttt tatccggctg gcgattctca atctcgcggg cgtgccatcg
      421 atcgtattcg gcttgttcgg ctttggcctg ttcgtgatct atctcgactg gaatgtttcg
      481 ctgctcgccg gttggttcac gctcgcgttc atggtgctgc cggtcgtcat caccgcatcg
      541 gaagagtcgc tgaagtcggt gccgaaaggt tttcgcgaag gctcgctcgc gctcggtgcg
      601 acgaagtggc agacgatccg caagaacgtg ctgccgtatg cgctgcccgg gattttgacc
      661 tcgtcgatcc tcggcattgc gcgggtcgcc ggcgagaccg cgccgatcat gttcacggcg
      721 gcctacgtcg tgcgcgacaa gctgccgtgg gacgtcgagc ggttcagcga cttctttttt
      781 caaggcgtga tggcgctgcc gtatcacatc tacgtggtgt catcgaagat cccgcaaaac
      841 gaatacacgg cgcgcgtcca atacggcacg gcgttcgtgt ttctcgcgct cgttgcgctc
      901 atcgccctca cgtccatcgt tctccgcaac aaacttcgca accgctacaa gtggtgacaa
      961 ccatgacgac catggaaccg acgcttccca aaaccgcgcc tgcctcgttg ccttcgcccg
     1021 ctgtgaacac cgcgccggcc gttcagcgct cccggcctgg aacgctctcc gcacccggag
     1081 aaaagccgat catcgagatc gatcgcgtga atttttccta cggttcgcag cgcgccctca
     1141 acggaatttc cctcaacatc gaagccaaaa aagtcacggc cttcatcggg ccgtccggct
     1201 gtggcaaatc cacgctgctt cgctgcctca atcggatgaa cgacctgatc gaaggcgcgc
     1261 gcgtgaccga tggcgcgatc cggattcgtg gcgtcgacat caac
//
LOCUS       DASPLT010001166         1162 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_178532, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001166 DASPLT010000000
VERSION     DASPLT010001166.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1162)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1162)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1162
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_178532"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1162)
                     /locus_tag="VEA63_11975"
     CDS             complement(<1..>1162)
                     /locus_tag="VEA63_11975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HYD84770.1"
                     /translation="VYCARKLGKDEHNKKDWYPHDKLEPILRETFGIIVYQEQVMQIA
                     QILSGYSLGEADLLRRAMGKKIKAEMDAQRERFVSGAKEGGLDKAKANEIFDLLNKFA
                     AYGFNKSHSAAYALVSYQTAYLKANYPVQFMAAVLTAELGNAEKVAHFIAESEAMGIT
                     VLGPDVNESRETFTPVFGKVPSSEFRVQSSPPETQSSEPGTVIPAPSDSRASIRFGLA
                     GIKGVGEQAAQKVIEERDRNGAYRDFDDFLSRLDGRSINKRVLEHLAKTGAFDFSGAS
                     RKKLFDGIDAAMAAVAAHARDKAAGQHSFFDMFADEKAGAAVPSRPGSALNSQLSTLN
                     SAEEDFSSAEKLAYEKELLGFYVSGHPLNAFAGLTEAIDTFPLVELLEQPDRT"
BASE COUNT          202 a          323 c          388 g          249 t
ORIGIN      
        1 agtgcggtcg ggctgttcga gaagttcaac gagaggaaac gtgtcgatcg cttccgtgag
       61 gccggcgaag gcgttgaggg gatggccgga gacgtagaag cccaaaagct ccttctcata
      121 ggcgagcttc tccgcggatg aaaaatcctc ctcggccgag ttgagagttg agagctgaga
      181 gttgagagcc gaaccagggc ggctggggac agccgcccct gccttctcgt cggcgaacat
      241 atcgaagaag ctgtgctggc cggcggcttt gtcgcgggcg tgcgcggcga cggcggccat
      301 ggcggcgtcg atgccgtcga agagtttctt gcgcgacgcg ccgctgaaat cgaaagcgcc
      361 ggttttggcg agatgctcga gcacgcgttt gttgatcgag cggccgtcga gacgcgaaag
      421 gaagtcgtcg aaatctcgat acgcgccgtt cctgtcgcgc tcctcgatga ctttttgcgc
      481 ggcctgttcg ccgacgcctt tgattcccgc caacccgaac ctgattgagg cgcgggagtc
      541 gctgggggcg ggaatcacgg ttccgggttc cgagctttgg gtttcgggcg gggaactctg
      601 aactctgaac tctgaactcg gaactttgcc gaaaactgga gtgaaggttt cgcgcgattc
      661 gttcacgtcc ggccccagca cggtgatgcc catggcctcg gattcggcga tgaagtgcgc
      721 gactttttcg gcgttgccga gttcggcggt gagcacggcg gccatgaact gcaccgggta
      781 gttggctttc aaatacgcgg tctgatagct cacgagtgcg taagcggcgg agtgagattt
      841 gttgaagccg taagcggcga acttgttgag caggtcgaag atctcgttgg ctttcgcttt
      901 gtcgaggccg ccctccttcg caccggacac gaaacgctcg cgctgcgcgt ccatctcggc
      961 cttgatcttc ttgcccatgg cgcggcgcag caggtccgcc tcgccgagcg agtagccgga
     1021 gaggatctgc gcgatctgca tcacctgctc ctggtagacg atgatgccga aggtctcgcg
     1081 caggatcggt tcgagcttat cgtgcgggta ccagtccttc ttgttgtgct cgtccttgcc
     1141 caatttgcgc gcgcaataga cc
//
LOCUS       DASPLT010001167         1091 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_218088, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001167 DASPLT010000000
VERSION     DASPLT010001167.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1091)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1091)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1091
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_218088"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..762)
                     /locus_tag="VEA63_11980"
     CDS             complement(<1..762)
                     /locus_tag="VEA63_11980"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HYD84771.1"
                     /translation="MKKSSVVRFAARVALGYVVIAAAWIAASDWLLRAWAMDGRMRAW
                     VETGKGWVFVLVTGVLLFVLMRRSIARLQAEAGAREATQSALQESEERFRFVVENIRE
                     VFWIAEPGDSLRRVLYVSPNYETDWGFSRERLLNEPGVWLDHIHPDDRESLLEAVKTK
                     QVLGTYDEQFRVVRSDGAVRWVRDRAFPVRGSDGTIRRIVGMAEDITERKSLEEQFLR
                     AQRMEAVGTLAGGIAHDLNNILAPMLMAAGLLKEKI"
BASE COUNT          198 a          407 c          275 g          211 t
ORIGIN      
        1 gatcttttcc ttcagcaaac ccgccgccat cagcatcggc gcgagaatat tgttcagatc
       61 gtgcgcaatc cctcccgcga gcgtgccgac agcctccatt cgttgggccc ggagaaactg
      121 ttcctcgagc gatttccgct cggtgatgtc ttccgccatc cccacgatgc gccggatcgt
      181 gccgtcggaa cctcgcaccg ggaacgcccg gtcacgaacc cagcgaaccg caccgtcgct
      241 tcgcacgacc cggaattgct cgtcgtaggt cccgagcacc tgcttcgtct tcaccgcctc
      301 cagcagtgat tcgcgatcat cggggtggat gtgatcgagc cagacgccgg gctcgttcaa
      361 cagccgttcc cgcgagaaac cccaatcggt ttcgtagttg ggactcacat acagcacccg
      421 tcgaagcgag tcgcccggct cggcgatcca gaacacctcc cggatgtttt cgaccacgaa
      481 gcgaaatcgt tcctcgctct cctgcagagc gctctgcgtc gcttcacgcg cgccggcttc
      541 cgcctgcaat cgcgcaatgc ttctccgcat cagcacaaaa agcagcacac ccgtcaccag
      601 cacgaacacc cagcccttcc ccgtctccac ccacgcgcgc attcgcccat ccatcgccca
      661 ggcgcgcagc agccagtcgg aagcggcgat ccacgcggcc gcaatcacca cgtagccaag
      721 cgcgacccgc gcggcgaaac gaaccacgga cgattttttc aacgtatgct cctcgtctag
      781 cgatgcaccc gggcccgcgg cgccgatcgc cgcgtaaaag gtgaatggct ttgaaaacag
      841 ttgcacatcg gacggccagc ctgtctcgcc agcccgaaat cggtaacttc gtctctccgg
      901 ctacgctgcg cgttttttgc atttccgcca gttacccgcc gttctgcccg gacatcgcct
      961 tcccgttccg gccatcccgg tcgcggtgaa caaaaacctt tccgcgacgg gccgcgctgc
     1021 ttttgctgtc ccctcgcatt cgtttccatg acctccgccg agatccgcca gagcttcctc
     1081 gatttcttcg c
//
LOCUS       DASPLT010001168         2378 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_198947, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001168 DASPLT010000000
VERSION     DASPLT010001168.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 2378)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2378)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2378
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_198947"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            185..883
                     /gene="nth"
                     /locus_tag="VEA63_11985"
     CDS             185..883
                     /gene="nth"
                     /locus_tag="VEA63_11985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HYD84772.1"
                     /translation="MPSPVETAAVINQRPYATRAERAEFIDRRLAELYPTPAVPLLHE
                     DAYTLLIAVLLSAQCTDKRVNEVTPRLFAIADTPEKMAKVPVEHIQEVIRPCGLSPQK
                     AQAISGLSKILMEKYGGRVPQTFEELEELPGVGHKTASVVMAQAFHVPAFPVDTHIHR
                     LAQRWKLTDGRSVEQTEADLKKLFPREHWNALHLRIIYYGREHCTARGCDGMECEICR
                     ELFPERKRAVVTRK"
     gene            995..1071
                     /locus_tag="VEA63_11990"
     tRNA            995..1071
                     /locus_tag="VEA63_11990"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1029..1031,aa:Val,seq:cac)
     gene            1171..1410
                     /locus_tag="VEA63_11995"
     CDS             1171..1410
                     /locus_tag="VEA63_11995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD84773.1"
                     /translation="MADPTTVIVAEIVARLEDSDFSPFAILLSDGSRVEIPSPDHCTV
                     TRLLRRIEVEHDDGKITIINPLHITKLELIHKPAA"
     gene            complement(1787..>2378)
                     /locus_tag="VEA63_12000"
     CDS             complement(1787..>2378)
                     /locus_tag="VEA63_12000"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HYD84774.1"
                     /translation="NPGADDNASGVVCLLALARAFAPLRLRRTIQFLSFGAEEQLSVG
                     SAAHVRSHRPTTADVGLVVNFDSVASPLGHFGISVAGEDGLARHAARQMLAHGLAAQV
                     HREITPFADQFPFNRAGIPSLYFFRSNFPGGRWQHHSQHDTLANVCGATVARLLEAAA
                     PLVAGLGARRTWPFRPTLPAAQRALARQLGRDLFGP"
BASE COUNT          473 a          707 c          752 g          446 t
ORIGIN      
        1 atggtctctc cctctcatcg aggggacata caaggcctgc cgctacgggg gacgcatcga
       61 gggtggagga gcgcgtgcat tcgaggttgt tttcgaaatg ggagcggcgc ttatttcaaa
      121 gcatcggaga atgtccgcga cgtggccgtg caaggaacgc gacgggtttt ctccagtctg
      181 tctcgtgcct tctcccgtgg aaaccgccgc tgttattaat caacgcccct acgcgacgcg
      241 cgccgaacgc gccgagttca tcgaccgccg gctggcggaa ctttatccga cgccggcggt
      301 gccgctgttg cacgaggacg cctacacgct gctgatcgcg gtgttgttgt cggcgcagtg
      361 cacggacaag cgcgtgaacg aggtgacgcc ccggttgttt gcgatcgcag acacgccgga
      421 gaagatggcg aaagtgccgg tggagcacat ccaggaagtc atccgcccgt gtggattatc
      481 gccgcagaag gcgcaggcaa tctccggact ctcgaagata ttgatggaaa aatacggcgg
      541 ccgcgtgccg cagacgttcg aggagttgga ggaattgccg ggcgtgggcc acaagacggc
      601 gagcgtggtg atggcgcagg cgtttcatgt gccggcgttt ccggtggaca cgcacattca
      661 ccgcctcgcg caacgatgga agctgaccga cggccgcagc gtggagcaga cggaggcgga
      721 cttgaagaag ctgttcccgc gcgagcattg gaacgcgttg cacctgcgca tcatttacta
      781 tggccgcgag cactgcaccg cgcgtgggtg cgacggcatg gagtgcgaga tctgccgcga
      841 gcttttcccg gagcggaagc gcgcggtggt gacgcggaag taggggttgc tgcgcaggcg
      901 ctcggaagcg agcacgccag cgccggtttc tgggcgaact tcaaaacaag gttgattcgc
      961 gcgggcgggc tgttcacgtt ctgcccccct gaggggacgc ttagctcagt tggttagagc
     1021 acctgcttca cacgcagggg gtcgttggtt cgagtccaat agcgtccacc atcttcagcg
     1081 gggtttggtc gtcccaaacg atttgcggca agaagtcgtt gcgcccggcg cttgcattga
     1141 cgcggatgag aaagtcggaa cactcgcggt atggctgatc ccactaccgt cattgtcgcg
     1201 gagatcgttg ctcgactgga agactctgac ttttctccgt ttgccatcct tctttcggac
     1261 ggctctcgcg tagagattcc ttcacctgac cattgcaccg tcacacggtt actccgccga
     1321 atcgaggtcg agcacgatga tggaaagatc acgatcatca acccgcttca catcaccaag
     1381 ctggagttga ttcataagcc cgcggcctaa gtgcaggcgc gccagacgtc gcgacgggct
     1441 tggctctacc actgcgacag gtctggaggt ccggatgctt acggtatgtt tgccgctgct
     1501 tttgaggcaa acgagggatc gggccgcaac ttcattggat gccaatgcac gatctccgag
     1561 tctcctgcga ataggggcag ttagagcaga tcaatcaaaa ctatagccgc gataaggcac
     1621 aaaaggcgcg aaaacgactt ggcggcctct tctgttcttg cgccttttgc gcctctttgc
     1681 ggccagaatt tggctatata atttttttag ccgctgtagc ggagatgagc gatgatgagc
     1741 ggttaaccct cttctcgttt gggctacctc gtagcccgtg agaccgtcac gggccaaaaa
     1801 gatcgcggcc tagctggcgc gcgagcgcac gctgagccgc cggcagcgtc ggccggaacg
     1861 gccaggttcg acgcgcgccg agtcccgcca ccaaaggcgc cgcagcctcg agcaggcgtg
     1921 cgaccgtcgc tccgcaaacg ttcgccaacg tgtcatgctg gctatgatgc tgccatcgcc
     1981 cacccgggaa attcgatcga aagaaataca gcgaaggaat gccggcgcgg ttgaacggaa
     2041 actgatcggc aaacggcgtg atttcccggt gcacctgcgc cgccagccca tgcgccagca
     2101 tctggcgtgc ggcatgtcgc gccaagccat cctcgcccgc caccgaaatc ccaaaatgtc
     2161 cgagcggcga agccacgctg tcgaagttca cgaccagccc gacatccgcc gttgtcggcc
     2221 ggtgcgaacg cacgtgagcc gcggatccga cggagagctg ctcctccgcg ccgaacgaga
     2281 gaaactggat cgtgcgacgc agccgaagcg gcgcgaacgc ccgcgcgagc gccagcaaac
     2341 aaacgacgcc ggaagcattg tcgtccgccc ccggattc
//
LOCUS       DASPLT010001169         1338 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_238069, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001169 DASPLT010000000
VERSION     DASPLT010001169.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1338)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1338)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1338
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_238069"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1072)
                     /locus_tag="VEA63_12005"
     CDS             complement(<1..1072)
                     /locus_tag="VEA63_12005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009509099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD84775.1"
                     /translation="MKARSKAFACLLVLFAAALLSRLHAVPLMENLGRGVVAVRAADS
                     EAFVSWRILGTDPADVAFNIYRSTNGDAPVLLNATPLTAATHYADASADLTQSNAYSV
                     APVLAGVEQPASVAFTLAANAPAQSYLRVPLQRPASGPDYTYSPGDSSVGDLDGDGEY
                     ELVVKWDPSNQQDNSFDGFTGNVYLDAYKLDGTRLWRIDLGRNIRAGAHYTQFIVYDL
                     DGDGRAEIACKTADGTISGTGEIIGDANADHRGANGKILAGPEFLTVFNGQTGAILAT
                     TNYLPPRGNIADWGDTTGNRVDRFLAGVAYLDGRRPSLLMARGYYTRAVVVAWDWRDG
                     QLTQRWTFDTGFSGGPYANYKGQ"
BASE COUNT          231 a          353 c          525 g          229 t
ORIGIN      
        1 cctggccttt gtagtttgcg taggggccgc cggagaaacc ggtgtcgaac gtccagcgtt
       61 gcgtgagctg gccgtcgcgc cagtcccacg cgacgacgac cgcgcgcgtg tagtagccgc
      121 gggccatgag cagcgagggg cgccggccgt cgagataggc gacgccggcg aggaagcggt
      181 cgacgcgatt gccggtggtg tcgccccagt cggcgatgtt gccacgcggc gggaggtaat
      241 tcgtggtcgc gaggattgcg ccggtttggc cgttgaagac ggtgaggaat tcggggccgg
      301 caaggatctt gccgttggcg ccgcggtggt cggcgttggc gtcgccgatg atttcgcccg
      361 tgccgctgat cgtcccgtcc gcggttttgc acgcgatctc ggcgcgacca tcgccgtcga
      421 gatcgtagac gatgaactgc gtgtaatgtg cgccggcgcg gatgttgcgt ccgaggtcga
      481 tgcgccagag gcgcgtgccg tcgagcttgt aggcgtcgag gtaaacgttg ccggtgaagc
      541 cgtcgaagga gttgtcctgt tggttcgaag gatcccattt gacgacgagc tcgtattcac
      601 cgtcgccatc gagatcgccg acggagctgt cgccgggact gtaagtgtaa tcggggccgg
      661 aggcggggcg ctggagcggg acgcgaaggt aggattgagc gggagcgttg gccgcgagcg
      721 tgaaggcgac gctcgcgggt tgttcgacgc cggcgaggac gggcgcgacg gagtaggcgt
      781 tggactgcgt gaggtcggcg gaggcgtccg cgtagtgcgt ggcggcggtg agcggcgtgg
      841 cgttgaggag cacgggagcg tcgccgttgg tggagcggta aatgttgaaa gcgacgtcgg
      901 cgggatcggt gccgaggatg cgccaactga caaacgcttc cgagtcggcg gcccggacgg
      961 cgacgacgcc acgtccaaga ttttccatga gggggactgc atgaagccgc gaaaggagag
     1021 cggcagcgaa caacacgagg agacaggcga acgccttgga acgagctttc ataggagggg
     1081 tagagggagc acgggtcgaa cgacacgcgt ggggggaatt cactcctgca cctgcccgcg
     1141 acgacgcacc acgtcgccag ctatttgaca gttcgcgttt gcgcagaatc gggttttatc
     1201 cggtgacgaa aagcccgcgc cgatgcagtt cgggcgcggg cgacgagacg aacgaagatc
     1261 tcgtggcgtc tagtttctgc cgaagtgtga cgcgagattt gaggcgaggg ccggactatc
     1321 gctatgagcg cgggcgcg
//
LOCUS       DASPLT010001170         1825 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_94842, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001170 DASPLT010000000
VERSION     DASPLT010001170.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1825)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1825)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1825
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_94842"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(67..231)
                     /locus_tag="VEA63_12010"
     CDS             complement(67..231)
                     /locus_tag="VEA63_12010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD84776.1"
                     /translation="MLALCDLCATALMDLGRIQPRSLRVIACKQAPTGGRAAGDPCTH
                     AQGYELGASA"
     gene            complement(384..1454)
                     /locus_tag="VEA63_12015"
     CDS             complement(384..1454)
                     /locus_tag="VEA63_12015"
                     /EC_number="3.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017979763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_function: GO:0050568 - protein-glutamine glutaminase
                     activity [Evidence IEA];
                     GO_process: GO:0006482 - protein demethylation [Evidence
                     IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0018277 - protein deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator protein-glutamate
                     methylesterase"
                     /protein_id="HYD84777.1"
                     /translation="MKKIRVLIVDDSATVRQTLKEILESDPAIAVMATASDPYVAVER
                     IKHEVPDVITLDVEMPRMDGLTFLDKLMRQCPVPVVVCSTLTERNSATALAALEKGAV
                     EIITKPKLGTKQFLEESRIRICDAIKSAALVRVKPLAGPPATVATPKLTADAMLEKPR
                     SNAMIQTTEKIVAIGASTGGTEALRVFLEALPADAPGVLIVQHMPEKFTANFAARLNT
                     LCRVTVKEAEDGDTVIRGQALIAPGNKHLLLKRSGARYYVEVRDGPLVSRHRPSVDVL
                     FRSTARYAGRNAVGVIMTGMGDDGAKGLLEMKEAGAYSIAQDEASCVVFGMPAEAIKL
                     GAVDKILPLSQISAKVVALCRE"
     gene            complement(1516..>1825)
                     /locus_tag="VEA63_12020"
     CDS             complement(1516..>1825)
                     /locus_tag="VEA63_12020"
                     /inference="COORDINATES: protein motif:HMM:NF013867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CheR family methyltransferase"
                     /protein_id="HYD84778.1"
                     /translation="CLLRSRDPARLEVRVAPELRRRVAFHRLNFMDADYAVKDRFEVI
                     FFRNVMIYFDKPTQEAVINKLCRNLAPGGYLFVGHSESLAGLDIPVSCVSPAVYQKTR
                     "
BASE COUNT          333 a          631 c          520 g          341 t
ORIGIN      
        1 ccatcgactt cgaagcgctg ggcgcgttgg tcgttcgcac gctgcgtccg gcgagttgaa
       61 catcgctcac gcgctcgcgc cgagctcgta gccctgcgcg tgagtgcaag gatcgccggc
      121 ggcgcggccg cctgtaggag cctgcttgca ggcgatcacg cgcaacgaac gcggctgaat
      181 cctgcccaag tccatcaacg cggttgcaca gagatcacag agcgccaaca cggaggaagc
      241 aggaggccac caagtatctg aaggtctttc ctgcagacga ttgccgacga cgacgaaggc
      301 ccgtttgcat caagtatctc tctcggtggt ctctatgtcg gggcctctgt ggcctctgtg
      361 ttcccaccgt ttagttcgtc gaatcactcc cgacacaacg ccaccacttt tgcggagatc
      421 tgtgagagcg gaagaatctt atccaccgcc cccagcttga tcgcttccgc gggcatcccg
      481 aacaccacgc agctcgcttc gtcctgggcg attgaatacg cgccggcttc cttcatctcc
      541 aacaacccct tggcgccgtc gtcgcccatt ccggtcatga tcacgccgac ggcgtttcgt
      601 ccggcgtagc gcgccgtgga gcgaaacagc acgtccaccg agggacggtg ccggctcacc
      661 aacgggccgt cgcgcacttc gacataatac cgcgcgccgc tccgcttgag caaaaggtgc
      721 ttgttgcccg gtgcgatcaa cgcctgcccg cggatcaccg tgtcgccgtc ctccgcctct
      781 ttcaccgtca cacggcacaa cgtattcaac cgcgcggcga agttcgcggt gaacttctcc
      841 ggcatgtgct gcacgatcag aacacctggt gcgtccgcgg gtaacgcttc caaaaacacc
      901 cgcaacgcct ccgtgccgcc ggtcgatgcg ccgatcgcga cgatcttctc cgtcgtctga
      961 atcatcgcgt tggaacgcgg cttctccagc atcgcatcgg ccgtgagctt cggcgtcgcg
     1021 acggtcgccg gcggaccggc caacggcttc acccgcacca gcgcggcgga cttgatcgca
     1081 tcgcaaatcc ggatacgcga ctcctcgaga aattgcttcg tgccaagctt cggtttcgtg
     1141 atgatctcga ccgcgccctt ttccaacgcg gcgagggccg tcgcggaatt tcgctccgtc
     1201 agcgtcgagc acaccaccac cggcacgggg cactgccgca tcaacttgtc gaggaacgtg
     1261 aggccgtcca tccgcggcat ctccacgtcg agcgtgatca cgtcgggaac ctcgtgcttg
     1321 atccgctcca ccgccacata cggatcgctc gccgtcgcca tcaccgcgat ggccggatcg
     1381 ctttcgagga tctccttcaa cgtctggcgc acggtcgcgc tgtcgtcgac gatgagcacg
     1441 cggatctttt tcacgcgcga atcctcgcca aaagcccggg cctgcgcagc gcaggcttca
     1501 tcgcagcgcc ggcgctcatc gcgtcttctg atacaccgcg ggactgacgc agctcaccgg
     1561 aatatcgagt cctgccagcg attcggagtg gccgacaaac agatagccgc ccggcgcgag
     1621 attgcggcac agtttgttga tgaccgcctc ctgcgtcggc ttgtcgaaat agatcatcac
     1681 gtttcgaaag aagatcacct cgaagcggtc cttcacggcg taatccgcgt ccatgaagtt
     1741 caaacgatga aacgccacgc gacgacgcag ctcgggcgcg acccgcactt ccagcctcgc
     1801 gggatcgcgg ctgcgcagca ggcat
//
LOCUS       DASPLT010001171         1565 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_137457, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001171 DASPLT010000000
VERSION     DASPLT010001171.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1565)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1565)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1565
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_137457"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            24..>1565
                     /locus_tag="VEA63_12025"
     CDS             24..>1565
                     /locus_tag="VEA63_12025"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HYD84779.1"
                     /translation="MLANNTYASGAPSDDAIVLSPFEVNTSKDRGFTAVNAGTATKLG
                     LDLKDLAAPYSVMTGEFIRAMGITDIRSAVFWSTGGSQVFDGQGSDPFGLNGANTVTT
                     STMYSIRGQTLNAGQQRNFFLTGSMGDTYNIERIDFGRGPNAVLFNVGANNALGGGIS
                     TQGKRARLDDDFANIGTLVGSWDYYRATIDVNKQLTDNLAVRANLMGQKRGGWMEDEF
                     EDRSGATLAATYRLAPRTELRIEGIYDTAQRATVPFPYFDLLSGWNGSTYFSGPLSEQ
                     IMSGAAPTANGAFLETRPGVNTYQAGSREGIARVTSPQYIWDPATNTLQNWIHTATTR
                     RGDENEWVPFYLGNTVFDRAGNNSILPFGNSNANGAGNRTPAVNQNGGQAAFLDMINL
                     PDSRFDRQVANSKFTVPGRRFSAMPDMPLYTQRWNDVNMNLTHAFTDNLFVEFAGDWN
                     RLYEGASNAHLNLRFGLIDLNETKPDGSANPNFKKAYSQTNNGLQFNYRTMTNWGMRA
                     NLGYIK"
BASE COUNT          327 a          495 c          475 g          268 t
ORIGIN      
        1 cggccgtgcc gacatcggcg cagttgcttg caaacaatac ttacgcatcg ggcgcaccga
       61 gcgacgatgc gatcgtcctc agcccgttcg aagtgaacac gagcaaggat cggggcttca
      121 ccgcggtgaa cgccggcact gcgacgaagc tcggtttgga tttgaaggat ctcgccgcgc
      181 cgtattcggt catgaccggc gagttcatcc gcgcgatggg catcacggat attcgctcgg
      241 cggtgttctg gtcgacgggc ggttcgcagg ttttcgacgg gcagggatcg gaccccttcg
      301 ggctcaatgg cgcgaacacc gtcaccactt cgaccatgta tagcattcgc gggcagacgc
      361 tgaacgccgg ccagcagcgc aatttctttc tcaccggcag catgggcgac acctacaaca
      421 tcgagcgtat cgacttcggc cgtggcccga acgcggtgct gttcaacgtc ggggcgaaca
      481 acgcgcttgg cggcggcatc agcacgcagg gcaagcgcgc ccgcctcgat gacgactttg
      541 ccaacatcgg cacgctcgtc ggctcgtggg actattaccg cgcgacgatc gatgtgaaca
      601 agcagttgac ggacaacctc gcggtgcgcg ccaacctcat gggacagaag cgcggcggct
      661 ggatggagga cgagttcgag gatcgcagcg gcgccacgct ggcagcgacg tatcggctgg
      721 cgccgcgcac ggagctacgc atcgaaggca tctacgatac agcgcagcgc gcgacggtgc
      781 cgtttcctta tttcgacttg ctcagcggtt ggaacggctc gacgtatttt tccggtccgc
      841 tctccgagca aatcatgagc ggcgcggcgc ccacggcgaa cggcgcgttc ctcgaaaccc
      901 ggccgggcgt gaacacgtat caagccggca gccgcgaagg catcgcgcgg gtgaccagtc
      961 cgcaatacat ctgggatccc gcgacgaaca cgctgcagaa ctggatccac accgcgacca
     1021 cgcggcgcgg cgacgagaac gagtgggtgc cgttttatct cggcaacacg gtgttcgatc
     1081 gcgcgggcaa caacagcatt cttcccttcg gcaacagcaa tgcgaacggc gccggcaatc
     1141 gcacgcccgc ggtgaatcaa aacggcggac aggcggcgtt cctcgacatg atcaatctgc
     1201 cggacagccg cttcgatcgt caggtcgcga attcgaagtt cacggtgccc gggcgccgtt
     1261 tctcggccat gcctgacatg ccgctttata cgcagcggtg gaacgacgtg aacatgaacc
     1321 tcacgcacgc cttcaccgac aacctcttcg tcgagttcgc cggcgactgg aaccggctgt
     1381 atgaaggcgc ctcgaacgcg cacttgaatc tgcgcttcgg actgatcgat ctcaacgaga
     1441 ccaagcctga cggcagcgcg aatccgaact tcaagaaagc atattcacaa accaacaacg
     1501 gcctccagtt caactaccgc acgatgacca attggggcat gcgcgcgaat ctcggttaca
     1561 tcaaa
//
LOCUS       DASPLT010001172         1005 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_94855, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001172 DASPLT010000000
VERSION     DASPLT010001172.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1005)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1005)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1005
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_94855"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..804)
                     /locus_tag="VEA63_12030"
     CDS             complement(<1..804)
                     /locus_tag="VEA63_12030"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HYD84780.1"
                     /translation="MNLTAAVECRGLVKDYPIGLRGVKLRAVGDLTFTVNRGEIFGLL
                     GANGCGKSTTLKLLAGIALPSGGQCRVLGESPQNPEVRRKIGYAPEAPEFYRFLTARE
                     LMRFYAEVSGPESASSAQSVAQALERVDLIDAADRKVGTFSKGMLQRVSLAQALVHDP
                     EVLILDEPVAGVDPLGIEKIGTLLRELRKAGKTILLTSHLLHQVAALCDRVAIMDRGR
                     VLTEVSLAELSREECAQTFATGPLSAEELYALRSWLAARGHSLQEITSGS"
     gene            complement(801..>1005)
                     /locus_tag="VEA63_12035"
     CDS             complement(801..>1005)
                     /locus_tag="VEA63_12035"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007360338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HYD84781.1"
                     /translation="MQIAKEFEVTGFVRNLADGRVHLEIEGRATEIDAFVQAIEERMH
                     GYVRKIERSGDSRAPQFTGFSIR"
BASE COUNT          196 a          344 c          279 g          186 t
ORIGIN      
        1 ggacccggac gttatttcct gcagggaatg tcctcgcgcc gccaaccagg agcgcagcgc
       61 gtagagttcc tcggccgaga gtggtccggt ggcgaacgtc tgcgcacatt cctcgcggct
      121 cagttctgcc agagaaacct ccgtgaggac acggccgcga tccatgatgg caacgcgatc
      181 acacagcgcc gccacctgat gaagcaggtg tgacgtgagc aaaatcgttt ttcccgcctt
      241 tctcagctct cgcagcagcg ttccgatttt ctcgatgccg agtggatcaa caccggcgac
      301 cggctcgtcc aaaatcaaaa cctccggatc gtgcaccagc gcctgcgcga gcgacacacg
      361 ctgcagcatg ccctttgaaa aagtgccaac cttccggtcc gccgcgtcaa tgaggtcgac
      421 gcgctcgagc gcctgagcaa cactttgcgc gctactcgcg ctttccggtc cactcacctc
      481 cgcgtaaaaa cgcatcagtt cgcgcgcagt caggaagcga tagaactccg gcgcttcggg
      541 cgcgtagcca atcttccgcc ggacctcagg attctgcggc gactcgccca agacgcgaca
      601 ctgcccgcca gacggcagcg cgatgcctgc gaggagtttc agcgtggtgc tttttccgca
      661 gccgtttgcg ccgagcagac cgaagatctc tccgcgattc actgtgaacg taaggtcgcc
      721 gaccgcgcgc aacttcaccc cacgcaggcc gatcggatag tccttcacca gcccgcgaca
      781 ttcgaccgcc gcggtgagat tcatcggatg gagaatcccg tgaactgcgg ggcacggctg
      841 tcgccgctac gctcgatctt gcgaacatag ccgtgcatcc gctcctcgat cgcttgcaca
      901 aacgcgtcga tctcggtcgc acggccttcg atctcgagat gcacgcggcc gtcggcgaga
      961 tttcgcacga acccggtaac ctcaaactcc ttcgcgatct gcatc
//
LOCUS       DASPLT010001173         2049 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_115242, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001173 DASPLT010000000
VERSION     DASPLT010001173.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 2049)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2049)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2049
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_115242"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..793)
                     /locus_tag="VEA63_12040"
     CDS             complement(<1..793)
                     /locus_tag="VEA63_12040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase assembly
                     protein OpcA"
                     /protein_id="HYD84782.1"
                     /translation="MPEIFDVLPGIEIPVGGIAKGLAQLWEETAASGRPAPASDDVKA
                     TQLNVVLHLGFGTTVDDGLEQFRTVLRFSQRYPSRVVVLCPLREDKGVTEMRAKVYGE
                     CFLGKSKGDTRCVEFVMLSYPRVARRHLENQVSICLSTDLPLYYWAHRFSQSGKLADY
                     TYLLTRAKRVLIDSSIAPADALTYPWPNPASLRDLVYSRLLPVRQTVGQFLARYESAL
                     LADGLRTVTVTHASALTAEGCVTSGWLRRRLAACGANIAAATFKVE"
     gene            complement(932..>2049)
                     /gene="zwf"
                     /locus_tag="VEA63_12045"
     CDS             complement(932..>2049)
                     /gene="zwf"
                     /locus_tag="VEA63_12045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007361157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HYD84783.1"
                     /translation="FAPIIRNLGASGLAAKYLGQPNHTKVIIEKPFGKDLASARALNT
                     TIRGVFEEHQVYRIDHYLGKETVQDLLVQRFANAIFEPLWNRNFIDSVQITVAEEVGV
                     GSRGGYYEQSGALRDMIQNHTMQLLALTAMEPPVSLDAEAIRDEKVKLLKAIQPLDLR
                     PNGDVARARYAAGMVGGKSVPGYLQEEGIAPQSATETYAALRLSINNWRWQGVPFYLR
                     SGKRMARRVSEIAVNFKRPPGTLFAEGEKFNLAANTLSFQIQPDEGLSFILNAKVPGL
                     ETRTQPVKMNFRYATTFGSNTPEAYERLVLDAMVGDGTLFIRGDETEASWKLCTPILE
                     AWAAAGREGLDEYPAGSWGPPNADALLAKNGHCWRQP"
BASE COUNT          383 a          714 c          567 g          385 t
ORIGIN      
        1 gctcgacctt gaacgtcgcc gccgctatgt tcgcgccgca cgccgccagg cgccgccgca
       61 gccagccgct cgtcacgcaa ccctcggcgg tcagcgcact cgcatgcgtc accgtcaccg
      121 ttcgcaagcc atccgccagc aacgccgatt cgtagcgcgc caaaaattgc cccaccgttt
      181 gccggaccgg cagcagccgc gaataaacca gatcgcgcaa cgaggccggg ttcggccacg
      241 gatacgtgag cgcatccgcc ggtgcgatcg agctgtcgat cagcacgcgt ttcgcccgcg
      301 tcagcagata cgtgtagtcc gccagcttcc cgctctgaga aaaacgatgg gcccagtaat
      361 acagcggcag gtccgtcgaa aggcagatcg acacctgatt ttccaaatgc cggcgcgcca
      421 ccctcggata actcagcatc acaaattcga cgcagcgcgt gtcacctttc gactttccga
      481 gaaaacactc tccgtaaact ttcgcgcgca tctccgtcac gcctttgtcc tcccgcaacg
      541 gacacagcac gaccacccga ctcggataac gctgcgagaa ccgcagcacc gtgcgaaatt
      601 gctccagccc gtcgtccacc gtcgtgccga agccgagatg gagcacgaca ttgagctgcg
      661 tcgccttcac gtcgtccgac gctggcgccg gccggccgct cgccgcggtc tcctcccaca
      721 gttgcgcgag ccccttggcg attccgccga caggaatttc aatcccaggc aatacgtcga
      781 aaatctcagg catgttcaga aactccaaat tccaagcact aagctccatt gaaactccaa
      841 gcctcaaact cccagctcaa agagacctca tccattgatc tttctttgga gcttggaact
      901 tggagtttga cgcttccgcg caacgcgcgg ctcagggttg ccgccagcag tggccgtttt
      961 tcgcgaggag cgcgtcggcg ttcggcggcc cccaggagcc cgcgggatat tcatcgagcc
     1021 cttcacgccc cgccgccgcc cacgcttcga ggatcggcgt gcagagtttc cacgacgctt
     1081 ccgtttcgtc gccgcggatg aagagcgtgc cgtcaccgac catcgcatcg agcacaagcc
     1141 gttcgtaagc ctccggcgtg ttcgagccaa acgtcgtcgc gtaacggaaa ttcatcttca
     1201 ccggctgcgt gcgcgtctcg aggccgggca ctttcgcgtt gaggatgaag ctcagtcctt
     1261 cgtcgggctg gatctggaac gacagcgtgt tcgccgcgag gttgaacttc tcgccttccg
     1321 cgaacagcgt gcccggcggc cgcttgaaat tcaccgcgat ctccgacacg cgccgcgcca
     1381 tgcgctttcc cgaacgcagg taaaacggca cgccctgcca gcgccagttg ttgatcgaga
     1441 gacgcagcgc cgcgtaagtc tccgtcgccg attgcggcgc gatgccctcc tcctgaagat
     1501 agcccggcac gcttttgccg cccaccatcc cggcggcgta acgcgcgcgc gcgacgtcgc
     1561 cattcggccg gagatcgagc ggttggatcg ctttcaacaa tttcaccttc tcgtcgcgga
     1621 tcgcttccgc gtcgagcgac accggcggct ccatcgccgt cagcgcgagc agctgcatcg
     1681 tgtgattctg aatcatgtcg cgcagcgcgc cgctctgttc gtagtaaccg ccgcgactcc
     1741 cgacgccgac ttcctccgcc accgtgatct gaacgctgtc gatgaaattg cggttccaca
     1801 acggctcgaa aatcgcgttg gcgaaccgct gcacgagcaa gtcctgcacc gtctccttgc
     1861 cgaggtagtg atcgattcga tacacctgat gctcctcgaa cacgccgcga atcgtcgtgt
     1921 tgagcgcccg cgccgacgca aggtccttgc cgaagggttt ttcgatgatc accttcgtgt
     1981 gattcggttg cccgaggtat ttcgccgcga gcccgctcgc gccgagattg cgaatgatcg
     2041 gcgcgaaga
//
LOCUS       DASPLT010001174         1641 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_53340, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001174 DASPLT010000000
VERSION     DASPLT010001174.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1641)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1641)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1641
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_53340"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1064)
                     /locus_tag="VEA63_12050"
     CDS             complement(<1..1064)
                     /locus_tag="VEA63_12050"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HYD84784.1"
                     /translation="MTLLCIAPAVAQQTNPPKPAAEAAPPADEILILSPFEVTADNSR
                     GYYAANTMSGTRLNTRLEDLASAMSVVTKEQMQDFGLLDINDIFAYETSTEGTANFTD
                     FVIDRRGNVVDNISDNPQGANRIRGIGAANTAFGNFQTSGRAPIDPSMLESVEISRGP
                     NSNIFGLGNAAGTVNLVPATANLFRDRAETQMRADSYDGYRGSLDVNRVLKKGVLAVR
                     GSAVFQRDGFERKPSGTDTRRYNGMVKFQPFKSTTLRAGYYHYDQNGTRANSITPRDA
                     VSYWRDSGAPSWDPSTFTVKKNGSVVGTYPVNTTLPDYFLQSTYVNHSQLFIDQEGLE
                     LWSVGQTSANDSANSPSTSIR"
     gene            1420..>1641
                     /locus_tag="VEA63_12055"
     CDS             1420..>1641
                     /locus_tag="VEA63_12055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 28-related protein"
                     /protein_id="HYD84785.1"
                     /translation="MNDHPAPQGVFTVRNFGARGDGRTLDTSAIHAAIAAAHAAGGGT
                     VLFPAGTYLSASIRLQSRITLAFSAGAVIE"
BASE COUNT          286 a          496 c          523 g          336 t
ORIGIN      
        1 cggatggacg tgctcggcga gttggcgctg tcgttcgcgg aggtttgtcc gaccgaccac
       61 agttccaacc cctcctggtc gatgaaaagc tgggagtgat tcacgtaggt cgactggagg
      121 aaataatcgg gcagcgtggt attgacggga taggttccga cgacggagcc gttcttcttg
      181 acggtgaacg tcgacggatc ccacgagggc gcgccagaat ctctccagta ggagacggca
      241 tcgcgcggcg tgatcgagtt cgcgcgcgtg ccgttctgat cataatgata gtaacctgcc
      301 cgcagcgtgg tgctcttgaa cggctgaaac ttcaccatgc cattgtagcg ccgggtgtcg
      361 gtgccggagg gcttgcgctc gaagccgtcg cgttgaaaca ccgcgcttcc tcgaacggcg
      421 agcacgcctt ttttcagcac gcggttcacg tcgagactgc cgcgataacc atcgtagctg
      481 tcggcccgca tctgcgtctc cgcacggtcg cgaaagagat tcgccgtcgc tggcaccaga
      541 ttcacggtgc cggcggcgtt gccgaggccg aagatgttgg agttcggccc gcggctgatc
      601 tcgacgctct ccagcatgct cggatcgatc ggcgcacgtc cgctggtctg gaaattaccg
      661 aacgccgtat tcgcggctcc gataccgcga atgcggttgg cgccctgggg attgtcggag
      721 atgttgtcga cgacgttgcc gcgccggtcg atgacgaagt ccgtgaaatt cgccgtgccc
      781 tcggtgctcg tctcgtaggc gaagatgtcg ttgatatcga gcaggccgaa atcctgcatc
      841 tgctccttcg tcacgacgga catcgcggaa gcgagatcct ccagccgcgt gttgagccgc
      901 gtgcccgaca tcgtgttcgc cgcatagtaa cccctcgagt tgtcggcggt gacttcgaac
      961 ggcgacagga ttaggatttc gtccgcagga ggtgcggcct cggctgcggg tttgggagga
     1021 ttggtttgtt gcgcgaccgc gggagcgata cacaacaaag tcatggtcag cggcagcacg
     1081 cggccgcaac gagccgcgtg aaatattgcg aaggggtggt tcatgtgtgg gtggggtcag
     1141 gctgagacgg cgcggcgcaa aaccgcagtt cgtcgggatg cgcgactgtc gaaaaccatc
     1201 aaagcgtcgc aaacgcgccc gcccttatac ggataaagca gcgctcgcgt tcacgttggg
     1261 tttggccgct ccggccgccg tgcattcgcg cgccggttta tccgtataag tttccgctca
     1321 cttctgttgc gcgttcgaac cgcgaatcgt gcaggaatgg ttcgacccac cccgcacgtt
     1381 ttccagtttt gatcaatgtc gccccctctc ccctcaccgt tgaatgacca tcccgcgccg
     1441 cagggggtgt tcaccgtgcg caatttcggc gcacgcggcg acgggagaac gctcgacacc
     1501 agcgcgattc acgcggcaat cgccgccgcg cacgcggcgg gcggcggcac cgtgcttttt
     1561 cctgccggca cttatctctc cgcttcaatc aggctgcaaa gccgcatcac gctcgcgttc
     1621 tctgccggcg ccgtgatcga a
//
LOCUS       DASPLT010001175         1289 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_94873, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001175 DASPLT010000000
VERSION     DASPLT010001175.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1289)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1289)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1289
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_94873"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..510
                     /locus_tag="VEA63_12060"
     CDS             <1..510
                     /locus_tag="VEA63_12060"
                     /inference="COORDINATES: protein motif:HMM:NF020804.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small ribosomal subunit Rsm22 family protein"
                     /protein_id="HYD84786.1"
                     /translation="EPGTHAVSRQLGTIRDELVAANRFSVVAPCTHQLACPALAPGKE
                     RDWCHFFAPPPSEIFANPDWVKFGQRAGIDLRSLPYAFIALDRSPDRPATTDLSRVIG
                     RPEHFKPYARVLNCDATGLTELELPKRADPALFKQLERTKAPLVYRWRREGEKLLGGE
                     PLAPAPDSA"
     gene            complement(954..>1289)
                     /locus_tag="VEA63_12065"
     CDS             complement(954..>1289)
                     /locus_tag="VEA63_12065"
                     /inference="COORDINATES: protein motif:HMM:NF018178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HYD84787.1"
                     /translation="VLDNLKAKDNKEHDHLAASLYDIALGTNARPKPVGEWNEGRVVV
                     RGWHIEHWLNGEKVVDLDLASTDGKAAIAASKFKDWEKFATARRGHIALQDHGDKVSY
                     RAVKVRELK"
BASE COUNT          230 a          492 c          309 g          258 t
ORIGIN      
        1 gagcccggca cgcacgccgt cagccgccaa ctcggcacga ttcgcgatga actcgtcgcc
       61 gccaaccgct tctccgtcgt cgcgccctgc acgcatcaac tcgcctgccc cgcgctcgca
      121 cccggtaaag agcgcgactg gtgtcacttc ttcgcaccgc cgccctccga aattttcgcc
      181 aaccccgatt gggtgaaatt cggccagcgc gccggcatcg atctccgcag tctcccatat
      241 gcgttcatcg cgctcgatcg ttcgcccgac cgccccgcga ccaccgatct ctcccgcgtc
      301 atcggccgac ctgaacattt caaaccctac gcgcgcgtgc tcaattgcga cgcgaccggc
      361 ctcaccgaac tcgagctccc gaaacgcgcc gacccggcgc tcttcaagca gctcgagcgc
      421 acgaaagctc ccctcgttta ccgctggcgc cgcgaaggcg aaaaactcct cggcggcgaa
      481 cccctcgcgc ccgcccccga ctcagcgtag cgtcggtatc tacgtagccg cgccggttac
      541 ggcgcgggta gccgcaaaaa cgcagagaag gcgcaaagat gagttgagag cgcatctgct
      601 cttgcgcctt ttgtgcctct ttgcggccaa aggttcggct atagaatttt tcgatctgcc
      661 ctaaccccgc ccgcgatggc tcaatgcatc gccgcgttcg ccgcgcccaa gcagtccgtt
      721 ttaaccacgg atgaacacgg ataaacacag atacgaaatc atgactcatt cgagcaccgc
      781 acccggctcg gttttgaatc cgcatccgtg ttcatccgcg tccatccatg gttaaagaat
      841 tccgtccctc caccccgcga ttccctcatc tcacacgaac tcactctcgg cctccgacat
      901 tcgcgtgaat tcgtgtccat tcgtggttcc atcactcccc gcccacgttt acctcacttc
      961 aactcccgca ctttcaccgc gcggtaactc actttatcgc cgtgatcctg cagcgcgatg
     1021 tgcccgcgcc gcgccgtcgc gaacttttcc caatccttga acttgctcgc cgcgatcgcc
     1081 gcctttccgt ccgtgctcgc gagatccaaa tcaacgacct tctcgccgtt caaccagtgc
     1141 tcgatgtgcc agccgcgcac cacgacgcga ccctcgttcc actcgcccac cggcttcggc
     1201 cgggcgttcg tcccgagcgc gatatcgtag agcgacgccg cgaggtgatc gtgctccttg
     1261 ttgtccttcg ccttcagatt atcgagcac
//
LOCUS       DASPLT010001176         3142 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_199001, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001176 DASPLT010000000
VERSION     DASPLT010001176.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 3142)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3142)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3142
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_199001"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(515..904)
                     /gene="arr"
                     /locus_tag="VEA63_12070"
     CDS             complement(515..904)
                     /gene="arr"
                     /locus_tag="VEA63_12070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000237816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)--rifampin ADP-ribosyltransferase"
                     /protein_id="HYD84788.1"
                     /translation="MNSNATTFAQSFFHGTKADLKAGDCIAPGYASNYPNRANAVHVY
                     LTATLDAAIWGAELALGEGRGRIYVVEPTGAFEDDPELTDQKFPGNPSKSYRTLEALR
                     VLGEVISWTGHAPQQIKAMKDHIANLK"
     gene            complement(1026..1394)
                     /locus_tag="VEA63_12075"
     CDS             complement(1026..1394)
                     /locus_tag="VEA63_12075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD84789.1"
                     /translation="MVVVLLVSASRASPPKRLAHPHKALAQTDDAIAIQTRWSRSSGF
                     TILLANTGGDSMRFRIEYLTADARLCGAFLTGEALSLEEAVKAARSRTPPQGAQGFRI
                     YDSDAEGAALWGQSLAAHGA"
BASE COUNT          571 a          954 c         1029 g          588 t
ORIGIN      
        1 ttcaacgatg ttgcccgagg gatcttcaag caggatggcg cgcccggcgc cgctttcgct
       61 aaggtcgccg cgaaagcgcg cgccgacgga aggtctcgat aaaagaccac cgccgtgtcg
      121 atgtcagttg cgatagaacg aactgacgcc atttgcgcgt ctcctctagc gatgcggccg
      181 atttaaactt atggagccgc gccgcttcga agtgagctgc gaaacgcaag cgtttgataa
      241 aagttgagta acggcaaagc gcatcgagag ggccggccgg acgatacgcg cgaagctgca
      301 cttcgacacg ctgacgcgca gagcgaacca cggcgccatg acgcgacgcc gccagctcaa
      361 agagccattc gggtggccga cggccccgga ttttgcgcca cacaattgca gagggcgttg
      421 cttgttcccg cgaggcggcg cttaacagat gttcgcctgc tcagcgacat cggcgcgaga
      481 gaccgaccgg atcgcggcaa cgctttcggc cgggctattt cagattagcg atgtgatctt
      541 tcatcgcctt gatctgctgg ggcgcatgcc cggtccaaga aatgacctcg ccgagcacgc
      601 gcagcgcctc cagggtgcga taggatttgc tcggattgcc gggaaatttc tggtcggtca
      661 gctcgggatc gtcctcaaac gcgccggtcg gttcgacgac atagatgcgc cctcgcccct
      721 cgcccagagc gagttctgca ccccagatgg cggcgtcaag cgtagcggtc agatagacgt
      781 gaaccgcgtt tgcccggttt ggataattgg atgcgtaacc cggcgcgatg caatcgcctg
      841 ctttcaaatc agccttggtg ccatggaaaa aggactgcgc gaaggtcgtt gcgtttgagt
      901 tcattaaccc gctccgaaag ccgggaacga gcttggccta aacgcagccg cgcgcaattc
      961 ttgaaagcaa agcgccggcg cccaattttg gtgatggcgc tccgcgcaaa cgctccgcgc
     1021 aaaactcagg ccccgtgcgc ggcgaggctt tggccccaga gcgccgcccc ttcagcgtcg
     1081 ctgtcgtaaa tgcgaaagcc ttgcgcgcct tggggcgggg ttctgctccg tgcggccttg
     1141 acggcctctt cgaggctcag agcctcgccg gtgaggaagg cgccgcacag gcgggcgtcc
     1201 gccgttagat attcgatcct gaaccgcatc gaatcccctc ccgtattagc gaggaggatc
     1261 gtgaagccgc tggacctgct ccagcgcgtc tggattgcaa ttgcatcatc cgtttgagct
     1321 agagctttgt gcggatgggc gaggcgtttc gggggtgatg cgcgggaggc gctaaccagc
     1381 aaaacgacga ccatgctgag cgtcagctcc aggtgttggt actgagacgc caacactcgc
     1441 ggcgtcatat gtcgctacca ctgaaagggc gaggcttaga acgagcgaga ttgacaatgc
     1501 atcgccgttg gcgatttgga cgcgctagcg gcgaatgatc ttcgcttggc gattgctcgt
     1561 tagatcttct tatcgttctg ggtggactga ggggctgacg gcgcatcatc gcaaagtttg
     1621 cagaagcgca ttgaggcgcg tccgacgtct tgcacgcagg tgctcgcggg acgaaggcaa
     1681 gccttcgagc atagagtcag atggataggt gatcgcctca gtcgccgcca atcgcgccag
     1741 cagcgagcga cagggcccgc tcgggtccgg aaatcatagc ctactggaag cgcacgactt
     1801 gtccctgaag cgggtgatcg cggcggagtt cgcgcgccgc gcccgcgcgc ctccatgctg
     1861 cgcccgcctc cagtgcatcg aggttagcac cgcgttggcc gagccgaaca tagcggtcga
     1921 acacgaaggg cagtccctcc cccgccgcgt gagacggcgc attgagcacg tggaagtgaa
     1981 ggtgcggtgc ggtggaattg cctgaattgc cgacgccagc gctgtgctaa ctgaccgtcg
     2041 ctcgcgcaac gtttggcctt cggcgtcgta tgcggtgcgg gtgatacggt ctggcccagc
     2101 gggtcccttg cgtcgaaagc gtacatgcgc tcgacggcaa gctgccatag acagccgcat
     2161 tcatacgcac tgcaacgcaa tcggcgcggt tcaatgcatc gtagctgtat ggcgtgaggc
     2221 cgccgttagg atcagtcatc cgctcgggaa ggcccgtcgg gagataggcg tacgtcgtca
     2281 tggtggacca gtcgttcggc gaggcgagcc cagtggcctg gcggacgcga tcgacgagcc
     2341 cgtctgcatt gtagcgccac tcggtggccg cgcccgtccc gctcggcccg tcatagcggg
     2401 ttagccggcg ggaggcatcg taggcgcttt ccgacgagca agtgttcggc tgcctggcgg
     2461 ggctgctgac gggccaaacg cttgagcggt tgcgcaagga tttacggtag agctagtcga
     2521 gggccttcgc gggggtggcg ccgggctttg aggcgtgcgt ccgcgcgcgc caccgacaag
     2581 ggttcgagcg gcgattcggc gagcgcgcca ttcagcgcgc gatgtcgagc ggctcgtgcg
     2641 caatgaggcc attgtgtgca accggcaaga tcgaggctgt ctggaatgcg cgccccgagc
     2701 gcgaactcac caagcggaaa gctcgcttgc tgctttcgcc tggtcctatg agcgcaagcg
     2761 cccgcctcgc gcgcagagcg cttcgacctc gctgccgctg gccacgcgat taacggctca
     2821 gcccctcaag agcgcgatca tccgcttccg cctggcgcga ggaacgccag ctccgatgga
     2881 ttggctcttg gtcgctcccg aattgacggg caagccgtct tctccgagga cgccaacaaa
     2941 gaaagagagt ggtgactgaa tgagcttttc gcggcggcgt gtcattatgg gtttggcgtt
     3001 tggcgccttc tattcaccgc agccggcggg cgcgatcgtg gttcgtcacg acacgcccgc
     3061 cgcccgctat ctcgcccgcc cggatgcttt tccgcccatc gccgatctgc ccggcgaagg
     3121 ccacggcgtg ctgatcgcgc ct
//
LOCUS       DASPLT010001177         1144 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_137497, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001177 DASPLT010000000
VERSION     DASPLT010001177.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1144)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1144)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1144
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_137497"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..376)
                     /locus_tag="VEA63_12080"
     CDS             complement(<1..376)
                     /locus_tag="VEA63_12080"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HYD84790.1"
                     /translation="MLKAFITGITGQDGSYLCELLLEKGYEVHGLVHRPDALANSNIR
                     HLVTDAAVLGKRLFLHNGAFEDATHLRRIISRARPNEFYHLAGQSSPRLSLELPESTV
                     DSIGMATLRLLEILRDLPDPPKF"
     gene            583..>1144
                     /locus_tag="VEA63_12085"
     CDS             583..>1144
                     /locus_tag="VEA63_12085"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HYD84791.1"
                     /translation="MSPEFAALLAALSFRRGKNHAAIILMYRRLCLLAFVAIAVFPPL
                     LRSQSSPNSSLPVVESFFPGLKPLLEQAVRQSPRMIARSAAEALAANSLTVVRAEQLP
                     KIGGYVNYLPANYEQRKDKEGTNLTQKSAYAVNLTQPIYHWGALRDGTRIAELQQQSA
                     LGSTIETYRLLVGEIRACYLQLILKKA"
BASE COUNT          212 a          343 c          359 g          230 t
ORIGIN      
        1 gaaatttcgg cggatcgggg aggtcgcgga gaatctccag caggcgcagc gtcgccatgc
       61 cgatactgtc gaccgtgctc tccggtagct cgaggctgag tcgcgggctg gattgtccgg
      121 caaggtggta aaactcgttc ggccgcgcgc ggctgatgat gcggcgcaag tgagtcgcgt
      181 cctcgaacgc gccgttgtgg aggaagagtc gtttccccaa taccgcagcg tcggtgacga
      241 ggtgccggat gttgctgttg gcgagcgcgt cgggccgatg caccaggcca tgaacctcgt
      301 agcctttttc gagcagcagc tcgcagagat aagaaccgtc ctggccggtg atgccggtga
      361 taaaagcttt cagcatgtgc gaagggctgg ttatgcggag cgccgggccg agcgcaaagg
      421 gaaacgcggc ggtgcgcaaa cggcggtctc cgggctggcg tgctgattcc cggtagatgg
      481 cgcgcttgac gcaccacgtt gcgcgcgagc gcgcaaccta catgtcaagg aggcggcggg
      541 gcgctgttct cacgaactcc gaaggaacta aaagacttcc gcttgtctcc agaatttgct
      601 gcattgctcg ccgcgttgtc gtttcgacgc gggaaaaatc acgccgctat catcctgatg
      661 tatcgccgcc tttgtttatt ggctttcgtt gccatcgccg ttttcccgcc gctccttcgt
      721 tcgcaatcct cgcccaactc ttcgctgccg gtggtggaga gttttttccc cggcctgaaa
      781 ccgctgctcg agcaagcggt tcgccagtcg ccgcgcatga tcgcccgcag cgctgccgaa
      841 gcgctcgcgg cgaacagcct caccgtcgtt cgcgccgagc aattgccgaa gattggcggt
      901 tacgttaatt acctccctgc gaattacgag cagcgcaaag ataaggaggg gaccaacctt
      961 acgcagaagt ccgcctacgc ggtgaacctc acacagccga tttatcactg gggcgcgttg
     1021 cgcgacggca cgcgcatcgc cgaactccag caacagagcg cgctcggctc gaccatcgaa
     1081 acctaccgtc tgctcgtcgg cgagattcgt gcgtgttatc tgcagttgat cctgaagaaa
     1141 gccg
//
LOCUS       DASPLT010001178         2354 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_53512, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001178 DASPLT010000000
VERSION     DASPLT010001178.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 2354)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2354)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2354
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_53512"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1949)
                     /locus_tag="VEA63_12090"
     CDS             complement(<1..1949)
                     /locus_tag="VEA63_12090"
                     /inference="COORDINATES: protein motif:HMM:NF024627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitobiase/beta-hexosaminidase C-terminal
                     domain-containing protein"
                     /protein_id="HYD84792.1"
                     /translation="MLCSLSAIAHAQHFTGYDLIVPKSSTPNAVTVVDGTGITLPTNR
                     SFVIGIEGGTRNPIRFNSWTGTAAFPIVITNVHNAAERASVSDEVPGDPNATRRTAIY
                     LDDCKFIQLRGDNNPSFRYGIEVARAGGSVNGGGVRGVEVTGASSDVEISFLEIREIG
                     FAGIMVKQDPGCSAASSHPDLVYENISIHDNYIHDCGAEGLYIGFSFWTDDRCTTDVG
                     GFAHNIEGLRIYNNLVERCRWDGIQVGAATSDVAIYNNVIIDSGFDGAPGSGNTGVGV
                     QIGGGTTGLLHSNLIINCRNNGISLFGLGNNIVYNNLIYGAGGGMFIDNRPSPVPAGH
                     PAERQTQAGTPYYIYNNTFMNIAGHVLHTMSVETDNHFKNNLAVAPEPEEEHRFIWYN
                     RPNADHPYPTGQEGGNVFQRDTTGLSFADPANYDFRLLNSSTNGVDDGVALTTYVATD
                     FQGIPRPQGEAYDAGYSESGTLSVFLVASPPTTGNNGMLTASAINGTTPYTYAWSTGA
                     TTATISNLPPGLYSVTVTDAASNVVKKATYLFTGAEMGMPVSVTPATQVEAPTFTAPA
                     GTYPTSQAVTISSATSGATIRYTTNGSTPTPTSGTVYSGPVTVSSDGTLKAFAYKSGL
                     TASTVSVATYFIGSAPAAPTLTATSA"
BASE COUNT          404 a          596 c          886 g          468 t
ORIGIN      
        1 gcggaagtgg cggtgagcgt cggggcggcg ggcgcagagc cgatgaagta agtggcgacg
       61 gaaaccgtgc tggcggtgag accggatttg taggcgaagg ctttgagcgt gccgtcgctg
      121 ctcacggtga caggtccgct gtaaaccgtg ccgctcgttg gcgtcggcgt gctgccgttg
      181 gtggtgtagc ggatcgtcgc gcctgaagtc gcggaggaaa tcgtcaccgc ctggcttgtc
      241 ggataggtgc ctgcgggcgc ggtgaatgtc ggtgcttcca cctgggtggc gggcgtgacg
      301 gagacgggca tgcccatctc ggcgccggtg aacaggtagg tggctttttt caccacgttg
      361 ctcgcggcgt cggtgacggt gacgctgtag agaccgggcg ggagattgga gatcgtggcg
      421 gtggtggcgc cggtggacca tgcgtaagtg tagggcgtgg tgccgttgat ggcggaggcg
      481 gtgagcatgc cgttgttgcc ggtggtcggc gggctggcga cgaggaagac cgacagggtg
      541 cccgactcgg aatagccggc gtcgtaggct tcgccttgcg ggcgggggat gccttggaag
      601 tcggtggcaa cgtaggtggt caacgcgaca ccgtcatcga cgccgttggt ggacgagttg
      661 agcaggcgga aatcgtaatt ggcgggatcg gcgaaactca ggccggtggt gtcgcgttgg
      721 aagacgttgc cgccctcctg gccggtcggg tagggatgat ccgcgttggg ccggttatac
      781 cagatgaagc ggtgttcttc ctccggctcg ggagcgacgg cgagattgtt tttgaagtga
      841 ttgtcggtct ccacgctcat cgtgtgcagc acgtggccag cgatgttcat gaaggtgttg
      901 ttataaatat aatacggcgt gccggcctgg gtctgccgtt cggccgggtg accggcgggg
      961 acgggtgacg gacggttgtc gatgaacatg ccgccgcccg cgccgtagat caggttgtta
     1021 tagacaatgt tattgccgag gccgaagaga gagatgccgt tgttgcggca gttgatgatg
     1081 agattgctgt gaaggaggcc ggtggtgccg ccgccgattt ggacgccgac accggtgttg
     1141 ccgctgccgg gcgcgccgtc gaagccggag tcgatgatca cgttgttgta gatcgcgacg
     1201 tcgctggtcg cggcgccgac ctgaatgccg tcccaccggc agcgctcgac aagattgttg
     1261 tagatgcgga ggccctcgat gttgtgcgcg aagccgccga cgtccgtcgt gcagcggtca
     1321 tcggtccaga aggagaatcc aatatagaga ccctcggcgc cgcagtcgtg gatgtagtta
     1381 tcgtgaatgc tgatgttttc gtagacgaga tcgggatgcg agctggcggc ggagcagccg
     1441 gggtcctgtt tgaccatgat gccggcgaag ccgatctcgc gaatctcgag gaagctgatc
     1501 tcgacgtcgc tgctggcgcc ggtgacttcg acgccacgga cgccgccgcc attgacggag
     1561 ccgccggcgc gcgcgacctc gatgccatag cggaaggagg gattattgtc gccgcgcaac
     1621 tggatgaatt tgcaatcgtc gaggtagatc gcggtgcgac gcgtggcgtt gggatcgccg
     1681 ggaacctcgt cgctgaccga ggcgcgctcg gctgcgttgt ggacgttggt gatgacgatg
     1741 ggaaacgcag cggtgccggt ccagctgttg aagcggattg ggttgcgggt gccgccttcg
     1801 atgccgatca cgaagctgcg gttggtggga agcgtgatgc cggtgccgtc gacgacggtg
     1861 acggcgttgg gcgtggacga tttgggaacg atcagatcgt agccggtgaa gtgttgagcg
     1921 tgggcgattg cgctcaggga acaaagcact gcagcaacgc cgcagactcg cgagaaccag
     1981 gcaggggaac agggtgtttt cattgcgatc gctttctatc cacgggctat gccgaagctt
     2041 gtcgccgtgg tttgtgggga tgactaacct tcaagctggc agacccgcaa aggtgcaact
     2101 ggtgtgctta aaatacaccg gttcgtggct caatcgcatg agccgtcgct cgctgagaat
     2161 gggatgaaat ttcagcgtcg cgctcgcgcg tttggcgaag gggcgcggag ctgaaaaggg
     2221 ggcgcgcgaa gctggagctc gacaacgtcg tcgggctgat acgcagcgct ggcagaatag
     2281 cccgacggga cgtcgggctc caccttcgcg ttacaacatc gggtcgctct cgcccggacg
     2341 aaacgctcac gcgt
//
LOCUS       DASPLT010001179         4470 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_301220, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001179 DASPLT010000000
VERSION     DASPLT010001179.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 4470)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4470)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4470
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_301220"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..337
                     /locus_tag="VEA63_12095"
     CDS             <1..337
                     /locus_tag="VEA63_12095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD84793.1"
                     /translation="PQLMGSLLGEMETLWKKVTGNRQADFKATKRIEKAPWFSLVGAV
                     ANDFGAGKLASQNADDGSPPDHQYLLIGAKLGPVRLEEPGHLYAFANDAWHFYENNRG
                     SVTLRVTRL"
     gene            complement(363..1658)
                     /locus_tag="VEA63_12100"
     CDS             complement(363..1658)
                     /locus_tag="VEA63_12100"
                     /inference="COORDINATES: protein motif:HMM:NF033206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ScyD/ScyE family protein"
                     /protein_id="HYD84794.1"
                     /translation="MNLHRSPRSSSNSGACLARVAHAFLSATVAPAFAFVSPCSSRTS
                     RRIAIGAGCALLFAGSASAQFASRTIVGNLDNPRGLAFGADGALYIAEAGRVETPGAD
                     TPSITVRGMSLYYGETGAITRWEPGETSNVLSNLPSLYNPVSGEVTGVHGIGFGASGD
                     LYFTTGLGADPAVRTGPELNRLGHLMRLPAGGSAAESVADVSSYEGANNPAGGPLDSN
                     PFHLAVHSGGVLVADAGANAILNVNFDGSFDLVSTLTAVPPGIDAVPTSLALSETGEL
                     YISQLTGFPFPVGGAGVFRVDEAGLSLVGGGFTNVIDLTYGPDGMLYVLEFAHFGLLS
                     GDPTGGLWRVDPATGLSKLLMTEGLIAPTALTFDRFGNLYIANRGVMPGAGEVLQFHP
                     VPEPAVTGLAAAAGLAAFLFWKRRKHCAIARGEAVAIAR"
     gene            complement(2088..3746)
                     /locus_tag="VEA63_12105"
     CDS             complement(2088..3746)
                     /locus_tag="VEA63_12105"
                     /inference="COORDINATES: protein motif:HMM:NF013715.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HYD84795.1"
                     /translation="MPASPASEDAHLLRSISNQPLSRRLPVYLKLSGPGWLQSAITLG
                     GASLSGSLYLGVIGGFGLLWLQPLAMIMGIVMLCAVGYVTMATGRRPLEAINEFVNPV
                     LGYAWAAASLLASIVWSMPQYSLSIAVLRQNLLPELLGPAGAFGDFGGKLVPTLVILA
                     LSTYITWNYGKGGRGVRLYENTLKAVVGLIVLCFTGVVVSLSLSPAGLDWSGVAAGFI
                     PDFSSLTRPADAFLPLIAQTAPEHHAYWTDFIVAKQRDVIVAGAAAAVGINMTFLFPY
                     SILRRGWGREFSGLQKFDLATGMLIPFTLATSFVVIAAATQFHGVPQAGLPGADVAAL
                     TAKASPVQLGEFNGLLQGLEAERAAAGANATISLADQRLAAVLVNRDANNLASALEPL
                     TGHLFAQVVFGVGVLGMALSTITLLMLISGQVICAVFKQEQTGWLFRLGSLAAAAGAL
                     GPFFWSKAAFYLAIPASVFGFILMPIAYLSFFLLMNQERLLGDAMPRGARRVIWNVLM
                     GTTVILVGAASAFMISTKAGTGGIAAAGGLILAAVVAHFVRRRG"
     gene            4128..>4470
                     /locus_tag="VEA63_12110"
     CDS             4128..>4470
                     /locus_tag="VEA63_12110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD84796.1"
                     /translation="MIAVAVSVAVAQAQSLRIQLREDNPVLFGPGIKVPVFVEYFSPA
                     KVDVYVDGILAASDSAAPFEPVLTAVSEGQHRLEARALHSGAWVKSKELLIEVLNPTA
                     SKLSLHDFVSHR"
BASE COUNT          889 a         1353 c         1524 g          704 t
ORIGIN      
        1 tccgcaactg atgggctcgc tcctcggcga aatggaaacg ctctggaaaa aagtcaccgg
       61 caacaggcaa gccgacttca aggcgacgaa gcgcatcgag aaagcgccgt ggttttcgct
      121 cgtcggcgcg gtcgcgaacg atttcggcgc cggcaaactc gccagccaga atgcggacga
      181 cggctcgccg ccggatcacc aatacctgct gatcggcgcg aagctcgggc cggttcggct
      241 cgaggagccg ggacaccttt acgcctttgc gaacgatgcc tggcactttt atgaaaataa
      301 ccgcggcagc gtgacccttc gtgtcacgcg tttgtagccg cggcgacgcg caccgattcg
      361 aatcagcgcg cgatcgcgac cgcttcgcca cgagcgatcg cgcagtgctt ccggcgtttc
      421 cagaatagaa acgccgcgag tcccgccgcc gccgcaagtc cggtgaccgc cggctccggc
      481 acgggatgaa attgcaacac ctcgcctgcg ccgggcatca cgccgcggtt ggcgatgtag
      541 agattgccga agcgatcgaa cgtgagcgcg gtcggcgcga tcagcccttc cgtcatcagc
      601 agcttggaga ggccggttgc ggggtcgaca cgccacaagc ctcccgtcgg atctccggag
      661 agcaggccaa agtgcgcgaa ttcgagcacg tagagcatgc cgtccggccc ataggtgaga
      721 tcgatgacgt tcgtaaatcc gccgccgacc agggacaggc ccgcttcgtc gacgcgaaac
      781 acgcccgcac cgccgacggg aaatggaaac ccggtgagct ggctgatgta gagttcgccc
      841 gtttcgctca aagcgagcga agtcggcacc gcgtcgatgc ccggcggcac ggcggtcaac
      901 gtgctcacca gatcgaacga gccgtcgaag ttcacgttga gaatcgcgtt cgccccggca
      961 tcggccacga gcacgccgcc gctgtgaacg gcaagatgaa acggattcga gtcgagcggt
     1021 ccgccggcgg gattgttcgc tccttcataa ctggaaacat ccgccacgct ttccgccgcg
     1081 cttccgccgg ccggcaaacg catgaggtga ccgagccgat tcaactcggg tccggtgcgc
     1141 accgccggat ccgcgccgag cccggtggtg aaatagagat cgccgctcgc accgaagccg
     1201 atgccgtgca cgccggtgac ttcgccgctc acgggattgt agagagaggg cagattggac
     1261 agcacgttgc tggtctcgcc gggctcccag cgcgtgatcg cgccggtttc gccgtagtaa
     1321 agcgacatgc cgcgcacggt gatcgacggc gtgtccgcac cgggtgtttc cacgcgaccg
     1381 gcttccgcga tgtagagcgc gccatccgcg ccgaacgcga ggccgcgggg attgtcgaga
     1441 ttgccgacga tcgtgcgcga ggcgaattgt gccgatgcgc tgccggcgaa cagcaacgcg
     1501 caacccgcgc cgatcgcgat gcggcgcgac gtgcgcgatg agcaaggtga aacaaaggcg
     1561 aaggcgggcg cgacagtggc ggagaggaaa gcgtgagcga cacgcgcaag acaggctccg
     1621 gagttggagc tggaacgagg agaacggtgc aggttcatgg gaacgagatg cagcgcgcga
     1681 acaaccgtgg agcggatttg acgccgtgaa cggtttcgtg cgcttcgagc ttcgaggagg
     1741 aggtgggaaa aggagcgcat cgaagcgccc gtgggcgccg cgaagatctc cgcgaacggc
     1801 tgaagcttgg ctccgcaatc attggcggtt cagccgttac cggtcgacgc gccgattggc
     1861 ggtgaagctc cgaacgttgc tacggaattt taccgtgttt gcgctatcag ggcctgatgc
     1921 cgaactcagg cgtggaaggt ggagctcgac gttctcgtcg ggctgttttg cgaacgaaac
     1981 gctatcaggc ccgacgagga cgtcgggctc aaccttcgga gttcagcttt tctcaaggtc
     2041 gtaaccggtg acgcgaatcc ggcgtaggag cctgctgcgc cctcacttca tccgcggcgc
     2101 cgcacaaaat gcgcgaccac cgcggcgagg atcaacccgc cggcggcggc gatgccgccg
     2161 gtgccagcct ttgtgctgat catgaaggcg ctcgcggcgc cgactaagat tacggttgtc
     2221 cccatgagca cattccaaat cacgcggcgt gcgccgcgcg gcatcgcgtc gccgagcagt
     2281 cgctcctgat tcatcagcag gaaaaacgaa agatacgcga tcggcatgag aatgaagccg
     2341 aagaccgacg cagggatcgc gaggtagaac gccgccttgc tccagaaaaa cggcccgagc
     2401 gcgccggccg ccgccgccag gctgcccagg cgaaacagcc agcccgtctg ctcctgtttg
     2461 aacaccgcgc agatcacctg tcctgagatc agcatgagca gggtgatcgt cgacagcgcc
     2521 atgccgagca cacccacgcc gaacacgact tgggcgaaca ggtgcccggt cagaggctcg
     2581 agcgcggagg cgagattgtt ggcgtcgcgg ttgacgagca cggcggcgag gcgctggtcg
     2641 gcgagcgaga ttgttgcgtt cgcacctgca gcggcgcgtt cggcctcgag cccttgaagc
     2701 agtccgttga actcgccgag ttgcacaggc gaggctttcg cggtcaacgc ggcgacgtcg
     2761 gcgccaggca ggccggcctg cgggacgccg tggaattgcg tggcggcggc gatcaccacg
     2821 aaactcgtgg cgagcgtgaa cgggattaac attccggtcg cgagatcgaa cttctgcagg
     2881 ccgctgaact cgcggcccca gccgcggcgc agaatcgaat aagggaaaag gaaagtcatg
     2941 ttgatgccca cggcggcggc ggcgccggcg acgatcacat cgcgttgttt ggcgacgatg
     3001 aagtcggtcc agtaggcgtg atgttcgggg gcggtttgcg cgatcagcgg gagaaacgcg
     3061 tcggcgggcc gcgtcaaaga cgagaagtcg gggatgaacc cggccgcgac cccgctccaa
     3121 tcgagcccgg ccggcgacaa cgaaaggctc acgacgacgc cggtgaaaca caggacgatc
     3181 aggccgacga cggctttgag ggtgttttcg tagagtctga cgccgcggcc acccttgccg
     3241 taattccaag tgatgtaggt ggacagcgcg aggatgacga gggtaggcac gagctttccg
     3301 ccgaagtcgc cgaaagcacc ggcagggccg aggagttcag gcaggaggtt ttgccgcagc
     3361 accgcgatcg acagcgaata ctggggcatc gaccacacga tgctggcgag gagcgacgca
     3421 gccgcccagg cgtatccgag gaccggattc acaaactcgt tgatcgcctc aagcgggcgg
     3481 cggccggttg ccatcgtcac gtaaccgacg gcgcagagca tcacgatgcc catgatcatc
     3541 gcgagcggct gcaaccagag caggccgaag cccccgatca caccgagata gagactgccc
     3601 gagagcgacg ctccgccgag cgtgatggcg ctttgcagcc aaccggggcc ggagagcttc
     3661 aagtagacgg gcaggcgccg ggagagcggc tgattggaga tcgatcgaag cagatgggcg
     3721 tcttcggaag cgggcgatgc gggcatggtt ggggagggcg cggatgaagg gttcattcag
     3781 cgggagttgt gggggttaaa gcaaggcagc ctgaggcaag cgacggctgc ggcaaggcgc
     3841 gttctccatg aaagcgattc gctgagattg gacgttcggc acgagcgtca acgcggccat
     3901 cggtattgtg ccgaggcgcg ccgcggggaa agggccacta ggcaaaacgc tgacggcgtg
     3961 cgaaagtcgc gtctgccggg atgcagttct cgctcggccg attccctacc cctaccatga
     4021 aaacgctggc gtcccggagt gcatcgctcc gccgcggtgc atcgaatgaa gcattcgtca
     4081 ccaccacgct cctcggccgg cgcgcgcgaa cctggagatt cgctttcatg atcgcggtcg
     4141 cggtctcggt agcggtggcg caggcgcagt ccttgaggat ccagcttcgc gaggacaatc
     4201 cggtgctgtt tgggccgggc ataaaagtgc cggtgttcgt cgagtatttc agcccggcga
     4261 aagtcgacgt ttatgtcgac gggatcctgg cggcgagcga cagtgctgcg ccgttcgaac
     4321 cggttctcac cgccgtcagc gagggccagc atcggctgga ggcacgcgcc ctgcactcgg
     4381 gcgcatgggt gaagagcaag gagttgctca tcgaagtgct caatccgacc gcgtcgaagc
     4441 tcagcctgca cgacttcgtt tctcatcggt
//
LOCUS       DASPLT010001180         1035 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_115513, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001180 DASPLT010000000
VERSION     DASPLT010001180.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1035)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1035)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1035
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_115513"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..547)
                     /gene="rpsD"
                     /locus_tag="VEA63_12115"
     CDS             complement(<1..547)
                     /gene="rpsD"
                     /locus_tag="VEA63_12115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HYD84797.1"
                     /translation="MARYTGPTTRISRRFGQQILGSGKAFERRSYPPGQHGPKLRRKV
                     SEYAVGLNEKQKLRYIYGLLERQFRRVFEIAKKERGVTGERFLQLLETRLDSVVYLLG
                     LAKSRAAARQFVNHGHIRVNGHKVDIASYNVLAGDEIEVKNSPASRQLATRCLEENRI
                     RNVPGWLTMNAEAFKAVVNRLP"
     gene            complement(604..981)
                     /gene="rpsK"
                     /locus_tag="VEA63_12120"
     CDS             complement(604..981)
                     /gene="rpsK"
                     /locus_tag="VEA63_12120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HYD84798.1"
                     /translation="MKVRKAKGSKNVTSGVANVLASFNNTIVSITDGKGAVIAWSSAG
                     KCNFRGSRKSTAYAAQVVAQDAARNAMSHGLKDIVIRVSGPGLGRDSAVRALQAIGLE
                     ITSIIDVTPIPHNGCRPRKRRRV"
BASE COUNT          176 a          270 c          385 g          204 t
ORIGIN      
        1 tcgggaggcg attgacgacg gccttgaagg cttcggcatt catcgtgagc cagccgggaa
       61 cgttgcggat gcgattctcc tcgaggcagc gcgtggcaag ctggcgcgac gcgggcgagt
      121 tcttgacttc gatttcgtcg ccggcgagca cgttgtagga cgcgatgtcg accttgtggc
      181 cgttgacgcg aatgtgtccg tgattgacga actggcgggc ggcggcgcgg ctcttggcga
      241 ggccgaggag atagacaacg ctgtcgagac gggtctcgag gagctggagg aaacgctcgc
      301 cggtgacgcc gcgctccttc ttcgcgatct cgaagacgcg acggaattgg cgctcgagca
      361 ggccgtagat gtaacggagc ttctgctttt cgttgaggcc gacggcgtat tcggaaactt
      421 tacggcgaag cttcgggccg tgctggcccg gcggatagct gcggcgttcg aaggccttgc
      481 ccgagccgag aatttgctgg ccgaagcgtc ggctgatgcg ggtggttggt ccggtgtaac
      541 gagccattgt ggagtcctga ttttaaaagg tgagattaga gagcggaaaa ggcgcgagac
      601 accttagacg cggcggcgtt tgcgcgggcg gcagccgttg tgcgggatgg gcgtgacgtc
      661 gatgatcgag gtgatctcaa ggccgatcgc ttgcagggcg cggacggcgg aatcgcgacc
      721 gaggccggga ccggagacgc ggatgacgat gtccttcaaa ccgtgcgaca tggcgttgcg
      781 cgcggcgtct tgggcgacga cctgtgcggc gtaggcggtg gatttgcggg agccgcggaa
      841 gttgcacttg cccgcggacg accaggcgat gacggcaccc ttgccgtccg tgatcgaaac
      901 gatcgtgttg ttgaaagacg cgagcacgtt ggcgacgccg gacgtgacgt tcttcgaacc
      961 cttggccttg cggaccttga tcgtgccgag ctcttccttg aggagatctt cagcggtggg
     1021 ttgcttggca acgcc
//
LOCUS       DASPLT010001181         3160 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_95127, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001181 DASPLT010000000
VERSION     DASPLT010001181.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 3160)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3160)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3160
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_95127"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(75..947)
                     /locus_tag="VEA63_12125"
     CDS             complement(75..947)
                     /locus_tag="VEA63_12125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009507941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HYD84799.1"
                     /translation="MTAFKPPTLDQVCERALRLPCSPALLPRLIAALQSNDSSADDIA
                     GIITVDPGLASSTLRLANSAYFAGGVPVENVSEAVMRLGQHELYRLAALALVSRWESG
                     VGHGEAGDFCRHALCTAIAAEVLAETSERVSPQTAYTAGLVCDLGKLALTHACDAFYP
                     AIRAHCAAQQCTWTDAEAAVLGYHHVQVGARLLRAWNFPPVLVNVAEHYLRPSQAPAD
                     ALPLIAHLHAAKYVATSFGPGVGEDGFLFSLNESFLLEWGFTPELLQEAMPIVHERAT
                     ARLKDKLSQGAVTL"
     gene            complement(994..>3160)
                     /locus_tag="VEA63_12130"
     CDS             complement(994..>3160)
                     /locus_tag="VEA63_12130"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HYD84800.1"
                     /translation="SFKALDDAVNRLAAESILAQIGRDEGLVPAYSLLGDLRDLCATE
                     PALLGPIAATHTLLDQLLDSAKPFDDATLGGLRRVIDWLPNAIEAARIGGAIAPFAGA
                     NTASPAVANAAAATTAAAPEEHASEDVMLLLHLEDNRELLGEFYGEAVDHLQQIEAAL
                     LALDQQPDNPEALNSIFRSFHTIKGNAGFLGLVPMHTLAHEVESLLDLARNHQLRLNA
                     AIITEILRSRDALQALTQQVAAALETGQLPEQIIPVGHLIRAVKKLAANPGATITPSS
                     TPTASPVAVEVAAKPEAPKASSDEVITPFAPLATNASASSATTAKTGSAATAAASLKT
                     STGATVRVNTEKLDSLMDVVGELVIVQSQLLETARQHGENTGSPLQRNVAQLSRITKE
                     LQHTAMSLRMIPIKQSFQKMERLARDLARDFGKKVSFVTSGEDTELDRTVVEEIGDPL
                     VHMVRNALDHGLEPTAARVAAGKPETGTLHLKAYHQGSNIVIELQDDGRGIDPDKIYK
                     KAVEKGIVAPGEVLSRDATLALIFAPGFSTAEKVTSVSGRGVGMDVVKRNIEKLRGKI
                     EITSEIGHGSTFKIKLPLTMAIIDGLVVRVGDDRFILPSTSVQRALRPAREMISTVHG
                     QGEVLDLRGRLVPIHRLHRRFGIPHQAGNPWDGIVVIVEHSGKVSALLVDEMVSKQEV
                     VIKNLGAFMQSLPGVAGGAILGDGNIALILDPATLLQAA"
BASE COUNT          549 a          986 c         1045 g          580 t
ORIGIN      
        1 cgataatgca agcaggcagg cccggaggcc tgccgctacg agaccagcaa gcgcgcgcct
       61 ttcggcgcgg cgctttacag cgtcaccgcg ccttgcgaga gcttgtcttt caatcgcgcg
      121 gtcgcgcgtt cgtgcacgat cggcatggct tcctgcaaca gctcgggcgt gaagccccac
      181 tccagcagga acgattcgtt caacgagaaa aggaatccgt cttcgcccac ccctgggcca
      241 aacgacgtcg ccacatattt cgccgcatgc aaatgcgcga tcagcggcaa tgcgtcggcc
      301 ggcgcctgcg acggacgcag ataatgttcc gccacgttca ccagcaccgg cgggaaattc
      361 catgcgcgca gaagtcgcgc ccccacttgc acgtgatgat aaccgagaac cgccgcctcc
      421 gcatccgtcc acgtgcactg ctgcgccgca cagtgcgcgc ggatcgccgg ataaaatgcg
      481 tcgcacgcat gcgtcagcgc gagcttgccg agatcgcaca cgaggcccgc ggtgtaagcg
      541 gtctgtggcg acacccgctc gctcgtctcc gcgagcactt cggccgcgat cgcggtgcac
      601 aacgcatgac ggcagaaatc tcccgcttcg ccgtgcccca cgcccgactc ccatcgactg
      661 accagcgcca gcgccgccag ccggtaaagc tcgtgctgtc cgaggcgcat caccgcttcg
      721 gatacgtttt cgacgggcac gccacccgca aaatacgccg agttcgccag ccgcagcgtc
      781 gacgacgcga gacccggatc gacggtgatg atgcccgcga tgtcgtccgc actgctgtcg
      841 ttcgactgca gcgcggcgat cagccgcggc agcaaggccg gcgagcacgg aaggcgcagt
      901 gcacgttcgc aaacttggtc gagggtgggc ggtttgaaag cagtcacgat gaattgggtt
      961 cggtgaaacg atcgcggaat aacgacggcg attttacgcc gcctggagca acgtggcggg
     1021 atcgaggatc agcgcgatgt tgccatcgcc gaggatcgcg ccgccggcga cgccgggcag
     1081 gctctgcatg aaggcgccga gattcttgat cacgacttcc tgcttgctca ccatctcgtc
     1141 gacgagcaac gcggacactt tgccggaatg ttcgacgatg acgacgatgc cgtcccacgg
     1201 atttcccgcc tggtgcggga tcccgaaacg acggtgcaaa cggtgaatcg gcacgagccg
     1261 tccgcgcagg tcgagcactt cgccctgacc gtgaaccgtc gaaatcattt cgcgcgccgg
     1321 acgcagcgcg cgctgcaccg acgtgctcgg gaggatgaaa cgatcgtcgc ccacgcgcac
     1381 gacgagtccg tcgatgatcg ccatcgtgag cggcagcttg atcttgaacg tcgagccgtg
     1441 gccgatctcc gaggtgatct cgatcttgcc gcgcagtttc tcgatgttgc gcttcacgac
     1501 atccatgccg acgccgcggc cggagacgga cgtgactttc tccgcggtgg agaatcccgg
     1561 tgcgaaaatc aacgcgagcg tcgcatcgcg cgagagcact tcgcccggag cgacgatgcc
     1621 cttctcgacg gctttcttgt agatcttgtc ggggtcgatc ccgcggccgt cgtcctggag
     1681 ctcgatgacg atattgctgc cctggtgata cgccttgagg tgcagcgtgc cggtctcggg
     1741 tttgccggca gcgacgcgcg cagcggtggg ctcgaggccg tgatcgaggg cgttgcgcac
     1801 catgtgaacg agcggatcgc cgatttcctc gacgacggtg cgatcgagtt cggtgtcctc
     1861 gccgctggtg acgaaggaaa ctttcttgcc gaaatcgcgc gcgaggtcgc gggcgaggcg
     1921 ctccattttc tggaagcttt gcttgatcgg aatcatgcgc agcgacatcg cggtgtgctg
     1981 caattccttc gtgatgcgcg agagctgggc tacgttgcgc tggagcggcg agccggtgtt
     2041 ttcaccatgc tggcgggcgg tctcgagcag ctggctttgc acgatcacga gttcgccgac
     2101 gacgtccatc aacgagtcga gcttctccgt attgacgcgc acggtcgcgc cggtgctcgt
     2161 cttcaacgat gcagcggcgg tcgcggcact gcccgttttt gcggtggtcg cggagctcgc
     2221 ggacgcattc gtcgcgagtg gcgcaaacgg cgtgatcacc tcatcgctgc tcgctttcgg
     2281 cgcttcgggt ttcgcggcga cttcgaccgc cacagggctc gcggtcggcg tggatgaagg
     2341 ggtgatcgtg gcaccaggat tcgcggcgag ttttttgact gcgcgaatga gatggccgac
     2401 gggaatgatc tgctcgggca gttgtcccgt ctcgagcgcg gcggcgactt gctgcgtcaa
     2461 agcctgcaat gcatcgcgac tgcggagaat ttccgtgatg atcgccgcgt tcagccgaag
     2521 ctggtggttg cgcgcgagat cgaggagcga ctccacttca tgcgcgagcg tgtgcatcgg
     2581 cacgaggccg aggaagccgg cattgccctt gatcgtatga aaggaacgga aaatggaatt
     2641 cagcgcctcg gggttgtccg gctgctgatc gagcgcgagc agcgccgctt cgatttgctg
     2701 caggtgatcg acggcctcgc cgtagaattc gccgagcagc tcacggttgt cctcgagatg
     2761 caacagcagc atcacgtctt cgctggcgtg ttcttccggt gcggcggcag tcgtcgcagc
     2821 ggcggcgttc gcgaccgcgg gtgatgccgt gtttgcgccg gcgaatggtg cgatggcgcc
     2881 gccgatgcgc gcggcctcga tcgcgtttgg caaccagtcg atgacgcggc gcaagcctcc
     2941 cagcgtggcg tcgtcgaacg gtttcgcgga atcgagcaac tgatcgagca gcgtgtgcgt
     3001 cgcggcaatc ggcccgagga gcgcaggctc ggtcgcgcac aagtctcgca ggtctccgag
     3061 caagctgtaa gcgggcacaa ggccctcgtc gcgcccgatc tgggcgagga tggattcggc
     3121 ggcgagacga ttgacggcat cgtccagcgc tttgaaggac
//
LOCUS       DASPLT010001182         2860 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_178810, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001182 DASPLT010000000
VERSION     DASPLT010001182.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 2860)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2860)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2860
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_178810"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..362
                     /locus_tag="VEA63_12135"
     CDS             <1..362
                     /locus_tag="VEA63_12135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HYD84801.1"
                     /translation="VVPRWVMITCALTMAAGTAAGGWRIIRTMGHKMVKLQPVHGFAA
                     ETTAAVIIHGASSIGIPLSTTHVISTSIMGVGATKRLSAVKWGLVGKIVWAWVLTLPV
                     TGGLGYGIVTLLHRFGI"
     gene            complement(517..921)
                     /locus_tag="VEA63_12140"
     CDS             complement(517..921)
                     /locus_tag="VEA63_12140"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HYD84802.1"
                     /translation="MPRALAREMTLLNTLPPGLIAQADQDRLRQVLSNLIDNAIKYGR
                     SSGTVTISGRVLNETWAEVCVRDDGPGIPADARARVFERFYRADKARSREQGGTGLGL
                     AIVKHVVQAHGGDVRVESELGQGAAFYFTLPR"
     gene            complement(899..1705)
                     /locus_tag="VEA63_12145"
     CDS             complement(899..1705)
                     /locus_tag="VEA63_12145"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HYD84803.1"
                     /translation="MTFPAGLLLLLLAAAVFLWWRARSHARQLVEHHATGLAELRAAH
                     EAALHEHARRLQAVLDSMVEGIVVLDAQNHLVLSNRAAAQTFGFPEQAIGRALIEVVR
                     HHEVAALAARLEREPAVLGYELRIEGAPARFLQINAVALRDPAGEANGAVLVFHDITR
                     VRELEGVRQEFVANVSHELRTPLSLIKGATETLRDGIALCTIEQRLRRARPAVEHCGP
                     ACGPPDAADRRFAAARETRFRAGGVERAAVAVAARGAGSRRRPNAARARP"
     gene            complement(1827..2513)
                     /locus_tag="VEA63_12150"
     CDS             complement(1827..2513)
                     /locus_tag="VEA63_12150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HYD84804.1"
                     /translation="MKPKILVVDDEPDALEVLGFKLREAGCVPLFAADGAKAIAAVRA
                     EKPDLIVLDLMLPEIDGMEVCKILRRDPATVSIPIIMLTAKAGEMDRVLGLELGADDY
                     VTKPYSPRELVLRIRKLLKRAQAADETGEHLRIGPLEMDIPRHEALVDSRPVTLTATE
                     FNLLAILAKRRGRVQTRERLLQDVWGYETAIDTRTVDTHMRRLREKVGPVADYLETIR
                     GVGYRFKAES"
     gene            complement(2503..>2860)
                     /locus_tag="VEA63_12155"
     CDS             complement(2503..>2860)
                     /locus_tag="VEA63_12155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PhoU domain-containing protein"
                     /protein_id="HYD84805.1"
                     /translation="ELGREPQLKPYVDLPRMAAMSLEMLRDALTAFIERQPEKARFVV
                     PRDQEVDDLNRQLYRELSSFMIERPSNISRCLALMAVSKRLERIADHATNIAEEVVYL
                     YEFRDIRHAGLKANEA"
BASE COUNT          516 a          996 c          842 g          506 t
ORIGIN      
        1 tcgtcgttcc ccgctgggtg atgatcacct gcgccctcac gatggccgcc ggcacggccg
       61 cgggcggctg gcgaattatc cgcacgatgg gacacaagat ggtgaagctt caacccgtgc
      121 acggcttcgc cgccgaaacg actgccgccg ttatcattca cggcgcctcg tcgattggca
      181 tcccgttgtc gacgacgcac gtcatttcta cctcgatcat gggcgtcggc gccaccaaac
      241 gcctgagcgc agtgaaatgg ggactcgtcg gcaaaatcgt ctgggcgtgg gtgctcacgc
      301 ttccggtcac cggcgggctc ggctacggca tcgtcaccct cctccaccgc ttcgggattt
      361 agcggcgtcg cgttcgagaa aacgcgcgcc ttcagtggag agcgttttca aaaaagctgt
      421 ggccgttttg gagggacgac ctccgtgtcg tccacggccg gcacggaggc cgccctccat
      481 ccgagcggct acggaataat ccaaacggct tcgcgctcac cgcggcagcg tgaaataaaa
      541 cgccgcgccc tgccccaact cgctctcgac gcgcacgtcc ccgccgtgcg cctgcacgac
      601 gtgtttcacg atcgcgagac caagccccgt gccgccctgc tcgcgcgagc gcgctttgtc
      661 cgcgcgataa aaccgctcga agaccctcgc gcgcgcatcc gccggaatgc ccggcccatc
      721 gtcgcgcacg cacacctccg cccacgtctc gttcaacacg cgtccgctga tcgtcaccgt
      781 gccgctcgag cgcccatact tgatcgcgtt gtcgatcagg ttcgagagca cctgccgcaa
      841 ccgatcctga tccgcttgcg cgatcaaacc tggcggcagc gtgttcaaca gcgtcatttc
      901 acgggcgagc gcgcgcggca ttaggtcttc gacgacttcc tgcaccgcga gccgcaacgg
      961 cgacggctgc acgttcaaca ccacccgccc ggaatcgagt ttcgcgagca gcagcaaatc
     1021 gtcgatcagc agcgtcaggc ggtccgcatg ccggtccaca atgctcaaca gccggtcgag
     1081 cgcgacggag acgctgctcg atggtgcaaa gtgcgatccc gtcgcgcagc gtctccgtcg
     1141 cgcccttgat caacgacagc ggcgtgcgca actcgtggct gacattcgcg acgaactcct
     1201 gccgcacgcc ctcgagctcg cgcacgcgcg tgatgtcgtg aaacaccagc acagctccgt
     1261 tcgcttcgcc cgccggatcg cgcaaggcca ccgcgttaat ctgcaagaaa cgcgccggtg
     1321 cgccttcgat gcgcaactcg taaccgagca ccgccggctc gcgctccagc cgcgcggcaa
     1381 gcgccgcgac ttcgtgatga cgcaccacct cgatgagcgc gcggccgatc gcctgctcgg
     1441 gaaatccgaa cgtctgcgcc gccgcgcgat tcgataacac gaggtgattc tgcgcatcga
     1501 gcaccacaat cccttcgacc atgctatcga gcaccgcctg caagcgccgc gcgtgctcgt
     1561 gcaacgcggc ttcatgcgcg gcccgcaatt ccgcgagtcc cgtcgcatga tgctcgacga
     1621 gctgccgtgc atgactccgc gcgcgccacc aaagaaacac cgcggcagcg agcagcagca
     1681 aaagtagccc ggcaggaaac gtcatggttc gcgcccgccc cctgctgtgg gaggggcttt
     1741 acgccccgac ttatgccttg ggaatccagt cggagcgtaa agcccctccc acagctcgaa
     1801 ctgaatttcg tgcgtcacgc gtgaactcag ctttccgctt tgaagcgata ccccacaccg
     1861 cgaatcgttt cgagatagtc ggcgacgggg ccgactttct cgcgcaaccg gcgcatgtgc
     1921 gtgtcgaccg tccgcgtgtc gatcgccgtc tcgtagcccc acacatcctg cagcaatcgc
     1981 tcgcgcgttt gcacgcgacc gcggcgcttc gccaaaatcg cgaggaggtt gaactccgtc
     2041 gccgtcaacg tcaccggccg gctgtcgacc aacgcttcgt ggcgcgggat atccatctcc
     2101 agcggaccga tccgcagatg ctcgccggtt tcgtccgcgg cctgcgcgcg cttcagcagc
     2161 ttgcgtatcc gcaaaaccaa ttcacgcgga ctgtagggtt tggtcacgta gtcgtccgca
     2221 ccgagctcga ggccgagcac ccggtccatc tcgcccgctt tcgccgtcag catgatgatt
     2281 ggaatcgaaa ccgttgccgg atcgcggcga aggattttgc aaacctccat gccatcgatc
     2341 tcaggcagca tcagatcgag cacgatgaga tccggctttt cggcccgcac cgcggcgatc
     2401 gccttggcgc cgtcggcggc gaaaagcggc acgcagccgg cttcgcggag tttgaacccg
     2461 agcacttcga gcgcatcggg ttcgtcgtcg accaccagga ttttaggctt cattggcttt
     2521 cagtccggcg tgccggatgt cacgaaattc atagagataa accacctcct ccgcgatgtt
     2581 cgtcgcgtga tcggcgatgc gctcgagacg cttcgaaacg gccatcagcg cgaggcagcg
     2641 ggagatgttc gacggacgct cgatcatgaa gctcgagagt tcgcggtaga gctgacggtt
     2701 gagatcgtcg acttcctgat cgcgcggaac cacgaagcgc gctttttccg gctggcgctc
     2761 gatgaacgcc gtcagcgcgt cgcgcagcat ctcgagcgac atcgccgcca tgcgcggcag
     2821 gtcgacgtaa ggcttcaact gcggctcgcg cccgagttcg
//
LOCUS       DASPLT010001183         3592 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_218536, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001183 DASPLT010000000
VERSION     DASPLT010001183.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 3592)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3592)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3592
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_218536"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1373
                     /locus_tag="VEA63_12160"
     CDS             <1..1373
                     /locus_tag="VEA63_12160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD84806.1"
                     /translation="AKESRLLRLAIVPPRTAPETVVGVNHAYPTAELLGLAKQAGVTW
                     HRDWSLKWQHIEPSKGEYRWDVSDPQVDRVTTQGLNLMAMIPFPSTEWNSTAPDLATI
                     RKHSPRHGGGGQGDDQELLVRARWAWLPADPGELSEFVAKAVSRYRDRIQVWEFLNEA
                     LFTLYSLPSENVLKTKELKSYKEEDYFQLLRQTAPAIRAANPNARIMGGGMFPGDRSA
                     LEMVKLGLLDYIDILGVHDYPSAAARTGGEVKAPEALLSSMAILIETMRAHGGPKPLW
                     MTEFSYFGSDDLPRDPWIPIEGLWSEPQLLSEKQVADYVIRYVTIFLGRYGEKIFLHS
                     GCTGSVNRPGTESCLFADGAVRKVYPALAVFTELLGAKPEFVGDRINEGGYAFAFETG
                     EESLVVLWDPEEKAEVRVPRGIVCRDLMGRTIKSSSIRLGASPVYWSGASGKAAELLD
                     ASGPGR"
     gene            1393..2649
                     /locus_tag="VEA63_12165"
     CDS             1393..2649
                     /locus_tag="VEA63_12165"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HYD84807.1"
                     /translation="MRSSSSRRWWAVLPLVFVTYSLAYLDRANYGFAAAAGMAADLEI
                     SERMSALIGALFALGYCVFQIPGVAYAQRHSVKKLVFYCLLLWGAFATLTGVVSGAPA
                     LLAIRFGLGVAEAAVLPAMLVYVSNWFTRAERSRANSLLILGGPATVLWMSVLSGYLI
                     EAVGWRWMFILEGLPTIVWAFLWWWLVCERPADARWLSTDEKAALAAELEREQAEVAP
                     MRNYREAFRSRTVQVLAWQYFFWSLSFFGFVLWLPSIIKQASSMGIVQTGWLSAAPYL
                     LAMIAMPVVSTLSDRAGSRKRFIWPSLAIGAIAFGALYFTLATSFWWSFSLLIVAGAS
                     MYAPYGPFWAIIPETLPRNVVGGAIALVNSLGSLGAFVGGYAVGALTAVTGNPLASVL
                     LMSLSLGIAASLTGLIPARPAKTFSP"
     gene            2646..3590
                     /locus_tag="VEA63_12170"
     CDS             2646..3590
                     /locus_tag="VEA63_12170"
                     /inference="COORDINATES: protein motif:HMM:NF014841.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HYD84808.1"
                     /translation="MKIYAALPSGAAGHGDLLRQLTQGDEVWVADPQAMTASDFDAFA
                     EAEVAFGLIPIERLETARRLRWIQFVSVGIEAYRDVVWDEFPSITCTNLGGLYAEPMA
                     QTVLAGLLGLYRGIDQLAQAKPQREWQKERLHARIEILQEAHVLLLGNGNVNRRVRAL
                     LQPFGCRFTVFARTRGDIRTPAELDAVLSGIDVVCAALPDTPQTRGLLDASRLARLKA
                     GAIVVNVGRGSLIDEAALIERLTAGHLGGAVLDVTQREPLPEDDPLWNSPRTILTQHT
                     SAGSRHVMVSALAFFATNLEAYRSGRSLMNVVDWARGY"
BASE COUNT          568 a          972 c         1298 g          754 t
ORIGIN      
        1 aggcgaagga gtcccgcctg ctgcgtctcg cgattgtccc gccgcgcacc gcgcccgaaa
       61 cagtcgtcgg tgtgaatcac gcgtatccca cggcggagtt gctcgggctc gcgaaacagg
      121 ccggtgtgac gtggcatcgc gactggtcat tgaagtggca gcacatcgag ccgtcgaagg
      181 gcgaatatcg ctgggacgtc agcgatccgc aggtggaccg cgtgacgacg cagggcttga
      241 atctgatggc gatgattccg tttccgtcga ccgagtggaa ttccacggcg ccggatctgg
      301 cgacgatccg aaagcactcg ccacgacatg gcggcggagg acagggggac gatcaggagt
      361 tgctggtgcg cgcgcgctgg gcttggttgc cggcggatcc aggcgagctg agcgaattcg
      421 tcgccaaggc ggtgagccgc tatcgagacc ggattcaagt ctgggagttt ctgaacgaag
      481 cgctcttcac cctttattcg ctgccgagcg aaaacgtgct gaagacgaag gagctgaaga
      541 gctacaagga agaggactat ttccagctgc ttcgtcagac ggccccagcg attcgagcgg
      601 cgaatccgaa cgcgcgaatc atgggcggcg gaatgtttcc cggcgaccgc tctgcgctcg
      661 agatggtgaa gctcgggctg ctcgattaca tcgatattct cggggtgcac gattatccca
      721 gcgcggccgc gcgcacgggt ggcgaggtga aggcgcccga agcgttgttg tcgtcgatgg
      781 cgattttgat cgaaacgatg cgagcgcacg gcggaccgaa gccgctctgg atgacggaat
      841 tttcctattt cggatcggac gacctgccgc gcgatccttg gattccgatc gaggggctct
      901 ggtccgagcc gcagttgttg agcgagaagc aggtggctga ctacgtgatc cgttacgtta
      961 cgattttcct cgggcgttat ggagagaaaa ttttcctgca ctcgggctgc acgggctcgg
     1021 tgaatcgccc gggaacggaa agctgtctct tcgcggatgg ggcggtgcga aaagtatatc
     1081 cggcgctggc ggtgttcacc gaattgctgg gcgcgaaacc ggaatttgtg ggcgatcgga
     1141 ttaatgaggg cggctatgct tttgcgttcg agaccggaga ggaatcgctg gtggtccttt
     1201 gggatcccga ggaaaaggcg gaggtgcgcg tgcctcgcgg gatcgtgtgc cgcgatttga
     1261 tgggacgaac aatcaagtcc tcgtcgattc ggctcggggc gtctccggtg tattggagcg
     1321 gcgcatcggg caaggcggcg gagctgctgg acgccagcgg gccggggcgg tgaaccaccg
     1381 gaagatccaa gcatgcgctc ctcttcttcc cgacgctggt gggcggtgct gccgctcgtg
     1441 tttgtgacct acagcctggc gtatctcgac cgggcgaact acggtttcgc tgcggcggcg
     1501 gggatggccg cggatcttga gatcagcgag cggatgtcgg cgttgatcgg cgcgttgttc
     1561 gcgcttggct actgtgtttt ccagattccc ggggtggcgt atgcgcagcg tcacagcgtg
     1621 aagaagctgg ttttctactg cctgctgctg tggggcgcct tcgccacgct caccggcgtg
     1681 gtgagcggtg cgccggcgtt gctggcgatt cgtttcggcc tgggtgtagc ggaggcagcg
     1741 gtgctgccgg cgatgctcgt ctacgtcagc aactggttca cgcgcgcgga gcgctcgcgg
     1801 gcgaactcgc tgctgatcct cggcggtcca gcgacggtat tgtggatgtc ggtattatct
     1861 ggatacctga tcgaggcggt gggctggcgg tggatgttca ttctcgaagg gctgccgacg
     1921 atcgtgtggg cgttcctgtg gtggtggctg gtgtgcgagc ggccggcgga cgcgcggtgg
     1981 ttgtccacgg acgaaaaggc ggcgctggcg gcggaactgg agcgcgagca ggcggaggtg
     2041 gcgcccatgc gaaactatcg cgaggcgttt cgcagtcgaa cggtgcaggt gctggcgtgg
     2101 cagtattttt tctggagcct gagtttcttc ggtttcgtgt tgtggctgcc gtcgatcatc
     2161 aagcaggcgt cgtcgatggg catcgtccag accggctggc tttcggccgc gccttatttg
     2221 ctggcgatga tcgcgatgcc ggtcgtgtcg acactgtcgg accgggcggg ctcgagaaag
     2281 cgtttcatct ggccgtcact cgcgatcggc gcgatcgcgt ttggcgcgct ttatttcacg
     2341 ctggccacca gcttctggtg gtcgttttcg ctgctgattg tggcgggcgc gtcgatgtat
     2401 gcgccttacg ggccgttctg ggcgatcatc ccggaaaccc tgccgaggaa cgtcgtgggc
     2461 ggcgcgatcg cgctggtcaa cagcctcggt tcgctcggag cgtttgtggg cggatatgcg
     2521 gtcggcgcgc tcacggcggt aacgggcaac ccgctggcat cggtgctgct gatgtcgctc
     2581 tcactgggaa tcgccgcgtc tttgacggga ctgattccgg cgcggccggc gaaaactttt
     2641 tcaccatgaa aatttacgct gctcttcctt ccggggctgc aggtcacggc gatctgcttc
     2701 gtcagctcac ccaaggcgat gaagtgtggg tggccgatcc gcaggcgatg acggcgtcgg
     2761 atttcgacgc gtttgccgaa gcggaggtgg cattcgggct gatcccgatc gagcggctgg
     2821 agacggcgcg gcggttgcgc tggattcagt tcgtctcggt gggcattgag gcctaccggg
     2881 atgttgtgtg ggatgaattt ccttcgatca cctgcacgaa tctgggcggg ctttacgcag
     2941 agccgatggc gcagacggtg ctggcgggac ttctcgggtt atatcgcggc atcgaccagt
     3001 tggcgcaggc gaagccgcag cgagagtggc agaaggagcg cctgcatgct cgcattgaga
     3061 tactgcagga ggctcacgtg ttgctgctgg gaaacgggaa cgtgaaccgg cgcgtgcgtg
     3121 cgttgctgca gccgttcggc tgccgtttca ccgtgtttgc ccgcacgcgc ggcgatatcc
     3181 gcacgccggc agagctggat gccgtgcttt cggggatcga cgtagtgtgt gcggctttgc
     3241 cggatacacc gcagacgcgc ggcctgctgg atgcgtcgcg actggcgcgg ctgaaggcgg
     3301 gcgcgatcgt ggtcaatgtc ggccgaggct cgttgatcga cgaggcggca ctgatcgagc
     3361 gtttgaccgc cggtcacctc ggtggagcgg tgcttgacgt gacacagcgc gagccgctgc
     3421 cggaggacga tccgttgtgg aattctccgc gcacgatcct gacgcagcac acgtccgcgg
     3481 gatcgcggca cgtgatggtg tcggcgctgg cgtttttcgc gaccaacctc gaagcctacc
     3541 gttcgggacg ttcattgatg aacgtggtgg attgggcgcg cggttactga cc
//
LOCUS       DASPLT010001184         1109 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_279989, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001184 DASPLT010000000
VERSION     DASPLT010001184.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1109)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1109)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1109
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_279989"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(610..>1109)
                     /locus_tag="VEA63_12175"
     CDS             complement(610..>1109)
                     /locus_tag="VEA63_12175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD84809.1"
                     /translation="RARRKTGNLYDYFSVDYDYGQGCHIHSMCRQIDGCYNRVGEFFT
                     GTEGTVYGGGKLERYDRRTLTSPEFPTHADGQVQEHVDFLTSIVSSLPINDVKYVADS
                     TLTAIMGRISAYTGQLVRWTDLTNAGGPYYDLKLSPSAEDFEQGNVVAPPEGVVPIAG
                     AERPA"
BASE COUNT          205 a          299 c          378 g          227 t
ORIGIN      
        1 ccctggtagg cgcggaggct gcgttcctcg aagcgacgaa tgcggaccat cttgcgataa
       61 agctcgatgc gtccttcgcg gctcaggctt gcgttgatgg gcgcgctggc atggtcgcgt
      121 ttggcggtgt cgtccgcttt cttttcggcg gtggcggtcg tttttttgct cacggaaagg
      181 gaaaggtttg agttgaaagg gaaagtgtcc ggcggagcgc ggagaaatca agtgccgatt
      241 ggcttcggtg gcggcgctta tggcacgatg aagcgaggcg cgccggcgga gacgcggcgg
      301 ccgcgaagat gaagcgatgc gtttgcagcg acgctgcgca cgagcgtgcg ttgctgtgcg
      361 cgcgatccat cagccaacat ggaggcaggc acgcacggag gcgtgccgcc atgcttgggc
      421 tcaggcggag aaattcagtt caaccacgaa tggacacgaa cccacatgaa tgaaatcgag
      481 ttcggatgca gaattggatc gggacaccca acggtgatgc acacgatcgg tcccaaagct
      541 ttcctctgcc tcctctgaat tcgtgttcat tcgtgtcgat tcgcggtttc tcaactgcat
      601 gaatcaggct taggcggggc gttcggcgcc ggcgatcggc acgacgccct cgggcggggc
      661 gacgacgttg ccctgttcga aatcctctgc gctcggcgac aacttcaaat cgtaataggg
      721 accgccggca ttggtgaggt cggtccagcg cacgagttgc ccggtgtaag cggagatgcg
      781 gcccatgatc gccgtgagcg tcgagtctgc gacatacttc acgtcgttga tcggcagact
      841 cgagacgatg ctggtgagga aatcgacgtg ctcctgcacc tggccgtcgg cgtgagtggg
      901 aaactcaggg gacgtgaggg tgcggcggtc gtagcgctcc aattttccgc cgccatacac
      961 ggtgccctcg gtgccggtga agaactcgcc gacgcggtta taacagccgt cgatctggcg
     1021 gcacatgctg tggatgtggc agccctgacc gtaatcgtaa tcgacgctga agtagtcgta
     1081 gagatttccg gtcttgcggc gcgcgcgtc
//
LOCUS       DASPLT010001185         2182 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_95222, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001185 DASPLT010000000
VERSION     DASPLT010001185.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 2182)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2182)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2182
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_95222"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..984)
                     /locus_tag="VEA63_12180"
     CDS             complement(<1..984)
                     /locus_tag="VEA63_12180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD84810.1"
                     /translation="MTFAIRSRGLFRLAAALFGLLSLPSLHAQFQGLGSLWADDPIPF
                     SEAYALSSDGSTVVGVSVDGNGDLRSFRWTAATGMQALPVINSDWYGFGYGYSEAFAV
                     SHDGSVIVGSDYNLDEDQINGGTIQGYRWTAGATPAAPGTITGLPYVDPDYTNSIATG
                     VSADGSVVVGREGGDGRAFRWTAATGTVLIGDANSEANAVSADGSVVVGTSESNAFRW
                     TEAEGLETLAIPDANSSEAYAISADRSTIVGSFITNADLDAFNGIERAFAWTTSGVRE
                     LDALFAGDYVATHAHAVSADGRWAVGHSGESYAESAVLWDITTGNIWDLNEL"
     gene            complement(1421..>2182)
                     /locus_tag="VEA63_12185"
     CDS             complement(1421..>2182)
                     /locus_tag="VEA63_12185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HYD84811.1"
                     /translation="LLDVMMPGIDGYETCRRIKDNDRFRDVPLFFLTALTDTVDKVKG
                     FALGAVDYITKPIDPDEVLARVQAHLDLRSLHLALEERNEELDREVQRRIGAERSLAH
                     ALENAVVVVDRTGIMLFRTQAAQRLLARHNRQLTSAELRSWFDGGARFDTQGLRITRT
                     IDPDSPGRAMFAFDEIPVEPSPERLLALGLTPREAEILFWIAQGKTSPEIAIILDMAL
                     ATVKRHVHHILGKLGVETRLAAALKAMEIVGLPPP"
BASE COUNT          389 a          590 c          788 g          415 t
ORIGIN      
        1 aagttcgttg agatcccaga tgttgcccgt ggtgatgtcc cagaggacgg cgctttctgc
       61 gtaggattcg cccgagtggc caaccgccca gcgtccgtcg gcggagacgg cgtgagcgtg
      121 cgtcgcgacg tagtcgccgg cgaagagtgc atcgagttcg cggacgccgc tggtggtcca
      181 ggcgaaggcg cgttctatgc cgttgaacgc gtcaaggtct gcgttcgtta tgaaactgcc
      241 cacgatggtg ctgcggtcgg ccgagatggc ataggcttca ctggagttgg cgtcagggat
      301 cgcgagggtt tccaggccct cggcttcggt ccagcggaac gcgttgcttt cggacgttcc
      361 tacgacgacg gagccgtcgg cagagacggc gttggcttcg ctgttggcat cgccgatcag
      421 cacagtgcca gtcgcggcag tccagcggaa cgcgcgacca tcgccgcctt cgcgaccgac
      481 tacgacggag ccgtcggcgg agacgccggt cgcaatggag ttcgtgtaat ccggatcgac
      541 gtaaggcagt ccggtgatcg tgccgggggc ggcgggcgtg gcgccggcgg tccagcgata
      601 gccttggata gtgccgccgt tgatctggtc ttcgtcgagg ttgtagtcgg agccgacgat
      661 gacggagcca tcgtgggaca cggcgaacgc ttcgctgtaa ccgtagccga agccatacca
      721 atcggagttg atgacgggca gggcttgcat gccggtggcg gctgtccagc gaaacgaacg
      781 caggtcgccg ttgccgtcga cggaaactcc gacgaccgtg gagccatcgc tggaaagggc
      841 gtaggcctcg ctgaagggga tgggatcgtc ggcccagaga gatccgaggc cttgaaattg
      901 ggcgtggagt gacggaagcg acaggaggcc gaacagggcg gcggcgaggc ggaagaggcc
      961 gcgggagcgg atggcgaagg tcatggcgcg tcgggtgatt gaggggacgg gtgaatcgcg
     1021 ccagacgcag cgggcggctg caacgaggca aagctcgtag ttctacgagg tgggagacgc
     1081 gtctacgcgc tacgcgttta aggcgtatta taaataccgt ggcggcgatc gatgccgttg
     1141 cgggatgcga ttcgaagctg tggatagggc tttatgcccc gacgaacgag cgccggccag
     1201 tcgggctata aagcgctccc gcagtcgagc cgggctacac ttccgcttta actgctctag
     1261 aataatgtcg cagagataac tttgtagatt tcacacgttt ctgtctgccc ggacgccgag
     1321 gacgtgtccc taccagcgct gccgctcccc tcgatttatt cacgtttcct gtggaacgcc
     1381 attctagggc ggacgagttt tttgcgaccg cgccttctga tcagggcggc ggaaggccga
     1441 cgatctccat ggctttcagg gcggcggcga ggcgggtttc gacgccgagt ttgccgagga
     1501 tgtggtggac gtggcgtttg acggtggcga gcgccatatc gaggatgatc gcgatctcgg
     1561 gactcgtttt tccctgcgcg atccaaaaga ggatttcggc ttcgcgcgga gtgagcccga
     1621 gcgcgagaag tctttcgggc gagggttcga cgggaatctc atcaaaggcg aacatcgcgc
     1681 ggccgggcga atcgggatcg atggtgcgcg tgatgcgtaa accctgcgta tcgaaacgcg
     1741 cgccaccgtc gaaccagctg cgcagttccg cggaggtgag ttgtcgattg tgccgcgcga
     1801 gcaagcgctg cgcggcctgg gtgcgaaaca gcatgatgcc ggtgcggtcg acgacgacga
     1861 cggcgttctc gagtgcgtgc gcgagggagc gttcggcgcc gatgcgacgt tgcacttcgc
     1921 ggtcgagttc ctcgttgcgt tcttcgagcg cgaggtggag cgagcgcaga tcgagatgcg
     1981 cctgaactcg tgcgagcact tcgtcgggat cgatgggttt cgtgatgtaa tcgacggcgc
     2041 cgagagcgaa gcctttgacc ttgtcgacgg tgtcggtgag tgccgtgagg aaaaacaacg
     2101 gcacgtcgcg aaagcggtcg ttgtccttga tccggcggca ggtttcgtag ccgtcgatgc
     2161 ccggcatcat cacgtcgagc ag
//
LOCUS       DASPLT010001186         1145 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_95251, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001186 DASPLT010000000
VERSION     DASPLT010001186.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1145)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1145)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1145
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_95251"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1145
                     /locus_tag="VEA63_12190"
     CDS             <1..>1145
                     /locus_tag="VEA63_12190"
                     /inference="COORDINATES: protein motif:HMM:NF036578.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HYD84812.1"
                     /translation="LLKAGRNVLAAEVRWFGEHSPASEVHSPVPGWLVQGADGAELDT
                     PGDWRVIASTGVTPDTTAYISNAHQFLGYLEHVDLRAELLGWRERDFDDSGWARAVSY
                     GPARATEALWGVAPLRTLVPRDVPAMTELPGRFVRTIEARRLVPHRFGERPLRWTVAA
                     ESGGSILLEAESYETAFAEFEFSGGEGREVRITYAEALGEWIDEAGGRVWRKGAVRDD
                     FTRYEAHGYRDTLILRGGEQTWEPFHWRAFRFMRIDVRAGAEPVTLRDVRYRTCVHPQ
                     VFSARFDASDEQARRIFDVSVRTFRMGAHEIYDDSPYYEQLSYTADTRLEALASLHLC
                     NETALPRRTLRLFLDTLRPDGLLDARVPCQFARQTIPYFCLHWILMV"
BASE COUNT          180 a          322 c          423 g          220 t
ORIGIN      
        1 gtctgctcaa ggcggggcgc aacgtgctcg cggcggaagt gcgatggttt ggcgaacaca
       61 gcccggcgag tgaagtgcat tcgccggtgc ccggctggct ggtgcaaggt gccgacggcg
      121 cggagctcga taccccgggc gactggcgcg tgattgcgag cacgggggtg acgcccgaca
      181 cgaccgctta tatttccaac gcgcatcagt ttctcggata cctcgaacat gttgacctgc
      241 gcgcggagct gctcggctgg cgggagcgcg atttcgacga ttcgggttgg gcacgggccg
      301 tatcgtatgg gccggcgcgg gcgaccgagg cgttgtgggg cgtggcgccc ttgcgcacgc
      361 tcgtgccgcg cgacgttcct gcgatgacgg agttgcctgg acgttttgtg cggacgatcg
      421 aagcgcggcg gctggtgccg catcgcttcg gtgaaaggcc gctccgctgg accgtcgcgg
      481 ccgagtcagg tggttcgatt ctcctcgaag cggaaagcta cgaaacggct tttgccgagt
      541 tcgaattcag cggcggcgag gggcgggaag tgcgcatcac gtatgcggaa gcgttgggtg
      601 agtggatcga tgaagcaggc gggcgcgtgt ggcggaaggg cgcggtgcga gacgatttca
      661 cgcgttacga agcgcacggg tatcgcgaca cgttgatcct tcgcggcggt gaacagacgt
      721 gggagccgtt tcactggcgc gcgtttcgct tcatgcggat cgacgtgcga gcgggcgccg
      781 agccggtgac gttgcgcgac gtgcgctatc ggacgtgcgt gcatccgcag gttttttcgg
      841 cgcgcttcga tgcgagcgac gagcaggcgc ggcgaatttt cgacgtgagc gtgcggacgt
      901 ttcgcatggg cgcgcacgaa atctacgacg atagcccgta ttacgaacag ttgagctaca
      961 ccgcggacac gcggctcgaa gcgctcgcga gcctgcatct ttgcaacgag accgcgttgc
     1021 cccggcgaac attgcggctg ttcctcgaca cgttgcggcc ggatggcttg ctcgacgcgc
     1081 gcgtgccgtg ccagttcgca cggcagacga tcccgtattt ctgtctgcac tggattttga
     1141 tggtg
//
LOCUS       DASPLT010001187         1915 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_199404, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001187 DASPLT010000000
VERSION     DASPLT010001187.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1915)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1915)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1915
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_199404"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(57..1052)
                     /locus_tag="VEA63_12195"
     CDS             complement(57..1052)
                     /locus_tag="VEA63_12195"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HYD84813.1"
                     /translation="MNAPLPAGHRRILIVDDSRAIHDDFRKILGSKRPSKSLSAQEAE
                     LFGIPPSTAETLHTDYELDSAFQGEEALEKVKQAVAAGRPYALAFMDVRMPPGWDGVE
                     TTTRIWNVDADIQIVICTAYSDYSWSEMTARLGSSDRLVILKKPFDNIEALQLAAALT
                     EKWRLGHHARRQLADLEQLVEERTRELRATKDAAEVALPAPDRVLIDGRILLTGSRPL
                     ATYPWPDKRPPLSAHPTEGSSDARKQETLWEIKEGQLYLLAVSAFRADAAASSQPLGL
                     RDLMPERIQEGGVFADWFTGEFVVIERERVDAASHARIILRRFQIAAGRVLEEKR"
     gene            complement(1049..>1915)
                     /locus_tag="VEA63_12200"
     CDS             complement(1049..>1915)
                     /locus_tag="VEA63_12200"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HYD84814.1"
                     /translation="KSEAELTHAHRQLLESSRQAGMAEVATGVLHNVGNVLNSVNVSA
                     TLVIDQVRESKTSHVGKVAALLREHAASLATFIADDPKGSRIPGYLDALAEELANERR
                     TIVGELEHLCKNIEHIKDIVAMQQNYAKVSGLVEDVPLVDVVEDALRMNAGAFARHGL
                     EVIRDYQVTPIVGVERHKVLQVLVNLIRNAKYACDESGRPDKRIVISITQDGDRVRIG
                     VVDNGIGIPPENLTRIFAHGFTTRKQGHGFGLHSGALAAQEMGGSLLAHSDGLGHGAA
                     FTLELPLSRPAR"
BASE COUNT          317 a          520 c          699 g          379 t
ORIGIN      
        1 aggtggaaga acagcagagc ctcggatcaa ttttgcatcg tctgcacgtt ttgcggctat
       61 cgtttctctt cgagcacgcg tccggcggcg atttggaatc gccgcaggat gatgcgtgcg
      121 tgagacgcgg cgtcgacgcg ctctcgctcg atcacgacga actcgccagt gaaccaatct
      181 gcgaacactc cgccttcctg aatccgctcc ggcatgagat cgcggaggcc gagcggttga
      241 gatgacgcag cggcgtcggc ccgaaaggcg ctcaccgcga gaagataaag ctgtccttct
      301 ttaatctccc acagggtctc ttgtttacgc gcgtctgaag atccttcggt gggatgcgct
      361 gaaagcggcg gacgcttgtc cggccatggg taggtcgcca aagggcggga gccggtcagg
      421 agaattcggc cgtcgatgag aacacgatcg ggcgcgggga gagcgacttc cgcggcatcc
      481 tttgtggcgc ggagttcgcg cgtgcgctcc tcgacgagct gctcgaggtc cgcgagttga
      541 cggcgcgcgt gatgaccgag gcgccatttc tcagtgagcg cggcggcgag ctgcagcgct
      601 tcgatgttgt cgaacggctt cttcagaatg accagacggt ccgaggatcc gaggcgggcg
      661 gtcatctcgc tccaggaata gtcggaatac gcggtgcaga tgacgatctg gatatccgca
      721 tccacattcc aaatacgcgt ggtcgtctcg acgccatccc atccgggcgg catgcgaaca
      781 tccatgaatg cgagcgcgta gggtcggccg gcggcgacgg cctgcttcac cttttcgagg
      841 gcttcctcgc cttggaaagc ggagtcgagt tcgtagtcgg tgtggagtgt ttcggcggtc
      901 gacggcggga tgccgaagag ttcggcctct tgggcggaga gggatttgga cgggcgtttc
      961 gagccgagga ttttgcgaaa gtcgtcgtgg atcgcccgac tgtcgtcgac gatgaggatg
     1021 cggcggtggc cggccggcag cggggcgttc atcgggcggg gcgggagagc ggcagttcga
     1081 gggtgaaagc ggcgccgtgg ccgaggccgt cgctgtgtgc gaggagcgag ccgcccatct
     1141 cctgcgcggc aagggcgccg ctgtggaggc cgaagccgtg gccctgtttg cgggtggtga
     1201 agccgtgggc gaaaatacgc gtgaggtttt cgggagggat gccgatgccg ttgtcgacga
     1261 cgccgattcg gacgcggtcg ccgtcctgcg taatggaaat gacgatacgc ttgtcgggac
     1321 ggccggattc gtcgcaggcg tatttggcgt tgcggatgag gttgacgagg acctgcagca
     1381 ccttgtggcg ctcgacgccg acgatgggag tgacttgata atcgcggatc acttcgaggc
     1441 cgtggcgggc aaaggcgccg gcgttcatgc gaagcgcgtc ctcgacgacg tcaacgagcg
     1501 gcacgtcttc gacgaggccg gagaccttcg cgtagttttg ctgcatggcg acgatgtcct
     1561 tgatgtgctc gatgtttttg cagaggtgct cgagttcgcc gacgatcgtg cgccgttcgt
     1621 tcgcgagctc ttcggcgagc gcgtcgaggt aaccggggat gcggctgccc ttgggatcgt
     1681 cggcaatgaa cgtggcgagg ctggcggcgt gttcgcggag cagggcggcg acttttccga
     1741 cgtgggaggt cttcgattcg cgaacctgat cgatgacgag ggtggcggaa acgttcacgc
     1801 tgttgagcac gttgccgaca ttgtgcagca cgccggtggc gacctcggcc atgccggcct
     1861 gacgcgagct ttcgaggagc tggcggtgcg cgtgagtcag ctcggcctcg gattt
//
LOCUS       DASPLT010001188         1284 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_115697, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001188 DASPLT010000000
VERSION     DASPLT010001188.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1284)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1284)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1284
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_115697"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..835
                     /locus_tag="VEA63_12205"
     CDS             <1..835
                     /locus_tag="VEA63_12205"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HYD84815.1"
                     /translation="TNGQSALARLVWLAARPHTRIVHHHHTAADDGEQGTWSPLFRRV
                     LKHAPELVACSRFTQRSLAAALQRDDVQFLPYLTSSPVAAVGVQDRFYSPNSHLHFGF
                     MGRLVAEKGIDLICELSNRTDLADLTWHIHGSGAAYPAEFFASYPNVVYHGAYTAGSQ
                     QAAILESLDALVLFSTHNEGMPLSLMEAMSAGLPWIATDRGGTREMAVSEENCLVVDA
                     PHDTEKLAAAVREMAARIRRGATSRDEQRRAYDRLFAPPVVAELWCDFLEARELHAQS
                     A"
     gene            832..>1284
                     /locus_tag="VEA63_12210"
     CDS             832..>1284
                     /locus_tag="VEA63_12210"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HYD84816.1"
                     /translation="MTSSVSPFPAFTVPYLAREEPPSRGFGRTSATPFPKAESLDPAS
                     PPVRTSVLVNTYNHSQFIEDCIESLLVQTVLPDEIIVYDDASDDDTVERLRRYGSRIT
                     LIEGADEPTRPSYLCQAHAVQTAFARSTGRLVFLLDGDDRFKRDKIERY"
BASE COUNT          220 a          471 c          360 g          233 t
ORIGIN      
        1 taccaacggc cagagcgccc tcgcgcgtct cgtctggctc gccgcgcgtc cgcatacacg
       61 catcgttcac caccatcaca ccgccgccga cgacggcgaa caaggcacgt ggtcgccgct
      121 tttccgccgc gtgctcaaac acgcgcccga actcgtggcg tgcagccgtt tcacccagcg
      181 ttcgctcgcc gccgcacttc aacgcgacga cgtccagttt ctgccttacc tcacctcatc
      241 gcccgtcgcc gctgtcggcg tgcaggaccg tttctattcg ccgaattcgc acctgcactt
      301 cggtttcatg ggccggctcg ttgccgaaaa aggcatcgac ctgatttgcg agctgagcaa
      361 ccgcaccgac ctcgccgatc tcacgtggca cattcacggg agcggcgcgg cttacccggc
      421 ggaatttttc gcatcgtatc ccaacgtggt ctaccacggc gcctacaccg cgggttcgca
      481 gcaggccgcg attctcgaaa gcctcgacgc gctcgtgctg ttctcgacgc acaacgaggg
      541 catgccgctc agcttgatgg aagcgatgtc cgccggcctg ccctggatcg cgaccgatcg
      601 cggcggcacg cgtgaaatgg ccgtgagcga ggagaattgc ctcgtggtcg acgccccgca
      661 cgacacggag aaactcgccg ccgccgtgcg cgagatggcg gcgcgtatcc gccgcggtgc
      721 gacatcccgc gacgagcagc gccgcgccta cgatcgtctg ttcgccccgc cggtggtcgc
      781 cgagttgtgg tgcgacttcc tcgaagcacg cgaactccac gcccaatccg catgacgtcg
      841 tccgtgtcgc catttcccgc gttcaccgtg ccttacctcg ctcgcgagga gccgcccagc
      901 cgcggcttcg gacgaacgag tgcgacgcct tttcccaagg ccgaaagcct cgatccagcc
      961 tccccacccg ttcgcaccag cgtcctcgtc aatacctaca atcacagcca gttcatcgag
     1021 gactgcatcg agtcgttgtt ggtgcaaacc gtcctgccgg acgagatcat cgtctatgat
     1081 gacgcgagcg acgacgacac cgtcgaacgg cttcgccgtt acggcagccg cattactttg
     1141 atcgaaggcg ccgacgaacc cacgcgcccc tcgtatctct gtcaggcgca cgccgtgcaa
     1201 accgccttcg cgcgctcgac gggtcgtttg gtttttctcc tcgacggcga cgaccgcttc
     1261 aagcgcgaca aaatcgagcg ctac
//
LOCUS       DASPLT010001189         1133 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_115707, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001189 DASPLT010000000
VERSION     DASPLT010001189.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1133)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1133)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1133
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_115707"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..574
                     /locus_tag="VEA63_12215"
     CDS             <1..574
                     /locus_tag="VEA63_12215"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HYD84817.1"
                     /translation="DTVVIDDIADAVQHLIAKGEVDASRVAIMGASFGGWATYISLAK
                     YPELYRAGIAVAAVSNWRKTLRDDRWRFDNQVAFRFWKTLLERENFKTDEPFIDPLLR
                     NGEIKQPVFIIHGERDAIVHATEAREMLNALRKHNPNVQAKSFPRAGHGNWTSDERVI
                     YLNEISAFLEQHLPAVGTAEPAVAAAVGSP"
     gene            678..>1133
                     /locus_tag="VEA63_12220"
     CDS             678..>1133
                     /locus_tag="VEA63_12220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HYD84818.1"
                     /translation="MRKINWGVLSTAKIGLNKVVPALQRSTHGTVLALASRDAAKARA
                     AAGQLGIPRVHPSYEALLADPDVDAVYIPLPNHLHVPWSIKALEAGKHVLCEKPLGLT
                     ARDAQQLLDASKKYPQLKIAEAFMYRHHPQWQRARELIASGGIGELRTVS"
BASE COUNT          236 a          415 c          306 g          176 t
ORIGIN      
        1 cgacaccgtg gtcatcgacg acatcgccga cgccgtccaa cacctcatcg cgaaaggcga
       61 agtcgatgca tcgcgtgtcg ccatcatggg cgcgagcttc ggcggctggg ccacctacat
      121 cagtctcgcg aaatacccgg agctgtatcg cgccggcatc gctgtcgccg cggtctccaa
      181 ttggcgaaaa accctgcgcg acgaccgctg gcgcttcgac aatcaggtgg cgttccgctt
      241 ctggaaaacc ttgctcgaac gcgagaattt caaaaccgac gagccgttca tcgatcctct
      301 cctccgcaac ggcgaaatta aacaacccgt gtttattatc cacggcgagc gggacgccat
      361 cgtccacgcg actgaagcgc gcgaaatgtt gaacgccctc cgcaagcaca acccaaacgt
      421 gcaggcaaaa tcctttcccc gggccggcca cggcaactgg acgagcgacg agcgcgtcat
      481 ctacttgaac gagatcagcg ccttcctgga acaacacctc ccggccgtcg gcaccgccga
      541 gcccgcggtg gctgccgccg tcggttcacc ttaggaaaaa tcgcgacgcg cgagcggcgc
      601 gagcgacgcg gagccgcgac cgcgttatga cgcaacgccg cgtccatctt cgccgcgcat
      661 ccgtctagct ccccaacatg cgcaaaatca attggggcgt tctcagcacg gcaaagatcg
      721 gcctgaacaa agtcgtgcct gcgctccagc gcagcacgca cggcacggtc ctcgcgctcg
      781 cctcccgcga cgccgcgaaa gcacgcgccg ccgccggaca actcggcatc ccgcgcgtgc
      841 acccgtcgta tgaggcattg ctcgctgatc cggacgtgga cgccgtttac attccgcttc
      901 cgaatcacct tcacgtgccc tggtcgatca aggccctcga agccggcaaa cacgtgctct
      961 gcgaaaaacc gctcggcctc accgcccgcg acgcccagca gctcctcgac gcttccaaaa
     1021 aatacccgca gctcaaaatc gcggaagcct tcatgtatcg ccaccacccg cagtggcagc
     1081 gcgcccgcga actcatcgcc tccggcggca tcggcgaact gcgcacagtc agc
//
LOCUS       DASPLT010001190         1857 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_115711, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001190 DASPLT010000000
VERSION     DASPLT010001190.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1857)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1857)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1857
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_115711"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(6..>1857)
                     /locus_tag="VEA63_12225"
     CDS             complement(6..>1857)
                     /locus_tag="VEA63_12225"
                     /inference="COORDINATES: protein motif:HMM:NF017076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HYD84819.1"
                     /translation="RDINGALVFRELPLLTKLEPGQTGEFPELPALRTDETDPRKRWT
                     PALVGLIDTIDGGTRTVPAFAPAVGRTYLHLALELARLHLGVASDGVQIERDHIDFVK
                     ADGTIAARVPLHDRQLIDVNWFSKWASPRNLRVPFSTVFAYAEMLVSEKAEERAAAEE
                     FFAQEDFKDAIVLIGPVDPLEQDLAPTPFDEVPVPKVGIHGNMVKTIASGKYLQRLPS
                     WNGVAWLDFAIVLGLSAVVSWLAISGGARATLNKIASVAAMGAFTWLVFEVFRASHFV
                     LPLAPALGAAFTTSFAGVIWQLVEEEKQKGRIKGMFGTYVSPQLVERMVESGEDPQLG
                     GHEDEITAYFSDIQSFSSFSEKLGSGPLVELMNEYLTACTDIVQEEGGTLDKYIGDAV
                     VAMFGAPIALPDHAYRACVASQRVHVRLGELREKWKNEGGKWPEIVWRMQSRIGLNSG
                     RATIGNMGSRTRFNYTMMGDNVNLAARMESGAKAYGVYTMVTEATKAACEQHGGDRLV
                     FRFLDKIVVKGRSQPVPVYEVVGLKENVNEATRACCELFERGIERYLAQDWDAAIGLF
                     RQSAVLEPNVPGQTPGVETNPSLVLIERCEEMKREPPGEGWDGVYVMTSK"
BASE COUNT          362 a          704 c          448 g          343 t
ORIGIN      
        1 ctccctcact tcgaggtcat cacatacacg ccatcccaac cctcgcccgg cggctcgcgc
       61 ttcatctcct cgcaccgttc gatcaacacc agcgacggat tcgtttccac ccccggcgtc
      121 tgcccgggca cattcggctc cagcaccgcg ctctgccgaa acagcccgat cgctgcatcc
      181 cagtcctgtg caagataccg ctcgatccct cgctcgaaaa gttcgcagca cgcgcgcgtc
      241 gcctcgttca cattttcctt caacccgaca acttcgtaaa ccggcaccgg ctgagatcgc
      301 cctttcacca cgatcttatc gagaaaccga aacaccagcc ggtcgccgcc gtgctgttcg
      361 cacgccgcct tcgtcgcctc cgtcaccatc gtgtaaacgc catacgcttt cgcgccgctc
      421 tccatgcgcg ccgcgaggtt aacgttgtca cccatcatcg tgtaattgaa acgcgtgcga
      481 ctccccatgt tgccgatcgt cgcgcgcccc gagttcaacc cgatgcgcga ctgcatccgc
      541 cacacgatct ctggccactt gccgccttcg ttcttccatt tctcccgcaa ctccccgagc
      601 cgcacgtgca cgcgctgcga cgcgacgcac gcgcgataag cgtgatccgg cagcgcgatc
      661 ggcgcgccaa acatcgccac caccgcgtcg ccgatgtatt tgtccaacgt cccgccttcc
      721 tcctgcacga tgtccgtgca cgcggtcaga tattcgttca tcagctcgac gagcggcccc
      781 gatcccagct tctccgaaaa actcgagaac gattggatgt ccgaaaaata cgccgtgatc
      841 tcgtcctcgt gtccgccgag ttgcggatcc tcgcccgact ccaccatccg ctccaccaat
      901 tgcggcgaaa catacgtgcc gaacattcct ttgatccggc ctttttgctt ttcctcttcc
      961 accagctgcc aaatcacccc ggcaaagctc gtcgtaaacg ccgccccgag cgccggcgca
     1021 agcggcagca cgaagtgact cgcgcgaaac acttcaaaca ccagccaagt aaacgcgccc
     1081 attgcggcga cgctcgcgat cttgttcaac gtcgctcgcg ccccgccgct gatcgcaagc
     1141 caactcacca cagcggacag ccccagcaca atcgcgaaat ccagccacgc cacgccgttc
     1201 cacgacggca ggcgctgcag atattttccg gacgcgatgg tcttcaccat gttgccatga
     1261 atgccgacct tcggcaccgg cacctcgtca aacggcgttg gcgccaaatc ctgctcgagc
     1321 ggatcgaccg gaccaatcaa cacgatcgca tccttgaaat cctcctgcgc gaaaaattcc
     1381 tctgccgccg cccgctcctc ggccttttcc gacacgagca tctccgcata cgcaaacacc
     1441 gtggaaaacg gcacgcgtag atttcgcggc gatgcccatt tcgaaaacca attcacgtcg
     1501 atcaactggc ggtcgtgcag cggcacgcgc gcggcgatcg ttccatcggc tttcacgaaa
     1561 tcgatgtggt cgcgctcgat ctgcacgccg tcactcgcca cgccgagatg caaccgcgcg
     1621 agctcgagcg cgaggtgcag ataggtgcgc ccaaccgccg gcgcaaacgc gggcaccgtt
     1681 cgcgtgccgc catcgatcgt gtcgatcaaa ccgaccagcg ccggtgtcca cctcttccgc
     1741 ggatcggtct cgtccgtgcg cagcgccggc aactccggaa actctcccgt ctgtcccggc
     1801 tccagcttcg tcaacaacgg caactcacga aaaacgagcg caccgttgat gtcgcgg
//
LOCUS       DASPLT010001191         2359 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_238427, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001191 DASPLT010000000
VERSION     DASPLT010001191.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 2359)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2359)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2359
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_238427"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..370
                     /locus_tag="VEA63_12230"
     CDS             <1..370
                     /locus_tag="VEA63_12230"
                     /inference="COORDINATES: protein motif:HMM:NF015617.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046556 - alpha-L-arabinofuranosidase
                     activity [Evidence IEA];
                     GO_process: GO:0046373 - L-arabinose metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="non-reducing end alpha-L-arabinofuranosidase
                     family hydrolase"
                     /protein_id="HYD84820.1"
                     /translation="LFEAVEVYKVKGANQYLMIVEAIGARGRYFRSFTATSLGGSWTP
                     LAASESAPFAGVNNVTFANGNQWTNDISHGDLVRNNPDQTKEIDPCNLQMLYQGFDKN
                     ARPADYGLIPYRPAVLTLQR"
     gene            complement(482..555)
                     /locus_tag="VEA63_12235"
     tRNA            complement(482..555)
                     /locus_tag="VEA63_12235"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(519..521),aa:Arg,seq:cct)
     gene            655..1644
                     /locus_tag="VEA63_12240"
     CDS             655..1644
                     /locus_tag="VEA63_12240"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HYD84821.1"
                     /translation="MAKVVGTWEGGRVVEKSDGTRVYYIRKRLAGKLYEVSTGATTLK
                     AALAHLQRFQANPEGYSASGALPGQPVTIRAFQRDYLDYCKAKGNSQNWITQKRRYLS
                     WWREAFPDDLRNATREQLLACVNGATVPHNRIASLKAFCSWLRDVRGVLPKGQDATLD
                     LPIPAVKPAQWKKVRAITREVFAKTMEHVDPAYADILTMIAGTGMHTTEAARLAKGQG
                     SVSGTTLTIFHKGGAQHRMEVEPHVAQAATRVLARGSFSVSRLMKAIKEACDKAEVER
                     WAPGCLRHSFITWMVGDGASIEHVAAYTNHDVATLKRYYNVAPIPRPTIARAS"
BASE COUNT          413 a          800 c          749 g          397 t
ORIGIN      
        1 cctgttcgag gccgtggagg tctacaaggt caagggggcg aaccagtacc tcatgatcgt
       61 cgaggcgatc ggcgcccgcg gcaggtactt ccgctcgttc accgcgacga gcctcggcgg
      121 ctcgtggacg ccgctcgccg cgtccgagag cgctccgttc gccggcgtca acaacgtcac
      181 gttcgccaac ggcaaccagt ggacgaacga catcagccac ggcgacctgg tccgcaacaa
      241 ccccgaccag accaaggaga tcgatccctg taacctgcag atgctctacc agggcttcga
      301 caagaacgcg cggcccgccg actacggcct catcccgtac cggccggcgg tgctgacgct
      361 ccagcgctag cgggcgctcg caggcagtcc agggagccgg tcgggacttc cagagcccgg
      421 ccggctcctc tcgcgtccgc gatcggctct tcggccgtgc gcgtgcgcgc gtccagcgaa
      481 gcgaccccgg cgtgactcga acacgcgacc tgcggtttag gaaaccgccg ctctatccgg
      541 ctgagctacg gggccattaa ttatttcaac cacttaccgc gcaccactga accgctcagc
      601 gctgcatccc cactacgtcc ccacggcccg cccgaaaccg cgtagagtgg cgacatggcg
      661 aaggtcgtcg gcacttggga gggcggcaga gtcgtcgaga agtcggacgg cacgcgggtc
      721 tactacatca ggaagcggct cgccgggaag ctttacgagg tatccaccgg tgcgacgacg
      781 ctcaaggcgg cgctcgccca cctgcaacgg ttccaagcga acccggaggg ctactcggcc
      841 tccggcgcgc tccccgggca gccggtgacg atccgggcgt tccagcgcga ctacctcgac
      901 tactgcaagg ccaagggtaa ctcccagaat tggatcaccc agaagcgccg gtacctgtcg
      961 tggtggcgcg aggcgttccc cgacgacctt cggaacgcca cccgcgagca gctgctcgcg
     1021 tgcgtgaacg gcgcgactgt cccccacaac cggatcgcct cgctcaaggc gttctgctca
     1081 tggctccggg acgtgcgggg cgtgcttccc aagggacagg acgcgacgct tgacctgccg
     1141 atcccggcgg tgaaaccggc ccagtggaag aaggtgcgcg cgatcacgcg cgaggtcttc
     1201 gccaagacga tggagcacgt cgatcccgcc tacgccgata tcctgacgat gatcgccggg
     1261 accgggatgc acaccaccga ggccgcgcgg ctcgcgaagg gtcagggaag cgtgtccgga
     1321 accaccctga cgatcttcca caagggcggc gctcagcatc ggatggaggt ggagccgcac
     1381 gtcgcgcagg ccgcgacgcg cgtgctggcg cgaggcagct tctccgtctc ccgactgatg
     1441 aaggcgatca aggaagcctg cgacaaggcc gaggtcgaac ggtgggcgcc ggggtgcctg
     1501 cggcattcgt tcattacgtg gatggtgggc gacggggcga gcatcgagca cgtcgccgcc
     1561 tacacgaacc acgacgttgc gacgctcaag cggtactaca acgtcgcccc gatcccgcgt
     1621 ccgacgattg cgagagcgag ctagccaacc cacctccgcg cacagcgggc ctttattcca
     1681 gacacgcgga tcgggcaaac tcacgaaagc tggcgggttt acagcaaccc tgcccctctt
     1741 tgaaaactta ggatgtgaac gatagaccgg cggagggcgt catgctccgc cggtctgttg
     1801 cgatcttcac gctgccgccg cccgtgccgc acgctcctgc tcgttcttga tccggcccac
     1861 tttgatgttt gagatcaggc cgtcaaatac gtgctgcggg aggtgcggag ggcgctccgg
     1921 aagcggcgat cccacgggca tgctcggcca gtcaattagg ccgttcttgt ctctcggcca
     1981 cgtagcctca tcgttagctg ccggtgtctt ggtcgcggcc tcgcgcggtt cggaaatggg
     2041 cttgggatct tgacggctta cgccgccgcg aagatccgac acttcttgcg attccgactc
     2101 gtccggcccc ggccccgtag cttctgggtc atatctaggc tcgccgaaaa gccccggcgg
     2161 aacgcttgct gccgttgtcg ccgatgacga agctcgattc tcaggggtgg aggtgttcgc
     2221 aggaccagcc cccgagatcc ttgccgttgc tatggctcgg agccgcgcgg cgagaggact
     2281 caggtcatac gtgttcgtcg cgctcttttt cggccccatc catctgtgtt cgctagcgat
     2341 tattagcccc gcctccttc
//
LOCUS       DASPLT010001192         3250 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_137975, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001192 DASPLT010000000
VERSION     DASPLT010001192.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 3250)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3250)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3250
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_137975"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..972
                     /locus_tag="VEA63_12245"
     CDS             <1..972
                     /locus_tag="VEA63_12245"
                     /inference="COORDINATES: protein motif:HMM:NF013400.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD84822.1"
                     /translation="GGDLSDFNRLWQSTYRAIKSVDASLRVGGPSTGRAEWLEELLDF
                     GQENDCPPDYLITHIYNNDSAADHPLAPFDPTATTRGSKSPTFAMDIMRNVRALIDRR
                     GFKGELHWNEWGRSFHAVDHHREHPSEAAFIARLLGEVSQEADVFSYWCLSDIYDQVG
                     YGREAFFGGYGLLNLQGLRKPAYHTFELLAKLGTERVALSGPPLDSTCNAIVTTSGDR
                     AHILVYASDLADDTKRQSIDVSVSLPPGARAKQLHRIDSIENNVLARWHELGAPAYLS
                     REQTKELAADNALKPSSSPVTIENGHAQFTMESPGIALLEITLPPRA"
     gene            1274..1750
                     /locus_tag="VEA63_12250"
     CDS             1274..1750
                     /locus_tag="VEA63_12250"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HYD84823.1"
                     /translation="MKKLLFAAFLCLACSIGVSSAAENATPPNIVLILIDDLGYGDIG
                     PFGSTKNRTLALDRMAREGRKFTSYYSAPVCSPTRAQVLTGAYAQRVSIPLAVMPGQK
                     HGLNPAENTVAELLKARRYATLCIGKWHLGYETEFFPTHHGFDPPGPNVRPAAHAD"
     gene            2353..>3250
                     /locus_tag="VEA63_12255"
     CDS             2353..>3250
                     /locus_tag="VEA63_12255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007359986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HYD84824.1"
                     /translation="MNLSFPKRFTWGVAAAAPQIEGAAFTDGKGESVWDRFSRIPGKV
                     ANGDTPDVACDHYHRYKQDFALMRKLGVKHYRLSLAWPRIFSEGRGAVNQKGVDFYNR
                     LIDAMLAAGIQPWVTMFHWDLPQALEDIGGWRARETPDAFGTYADTIVRAYGDRVKHW
                     ITLNEMRCFTVLAYGDTIRPPGIVEPPQVVNQTYHHALLAHGHGVRAVREHGGRGAQV
                     GITDDSTITVPVNETEADIAAARTAFQELNIRSIDPIFRGGYSSTYLRITGKDRAKTQ
                     RGDFDLISLPTDFFGFNIYTGVF"
BASE COUNT          644 a         1217 c          774 g          615 t
ORIGIN      
        1 ggcggcgatc tctccgactt caaccgcctt tggcaatcca cctaccgcgc gatcaagtcc
       61 gtcgacgcca gccttcgcgt cggcggcccg tccaccggcc gcgccgagtg gctcgaggaa
      121 ttgctcgatt tcggacagga aaacgactgc ccgcccgact acctcatcac ccacatttat
      181 aacaacgaca gcgctgcaga ccatccgctc gcgccgttcg atccgaccgc caccacgcgc
      241 ggaagcaaat cgccgacgtt cgcgatggac atcatgcgca acgttcgcgc gctgattgac
      301 cgccgcggct tcaagggcga gctccactgg aacgaatggg gacgctcctt ccacgccgtc
      361 gatcaccacc gcgaacatcc gtccgaagcc gccttcatcg cgcgtctcct tggcgaggtc
      421 tcgcaggaag ccgacgtgtt ttcctactgg tgcctcagcg acatctacga ccaggtcggc
      481 tacggccgcg aagcgttttt cggcggctac ggcctgctca atctgcaggg cttgcgcaaa
      541 cccgcttacc acaccttcga actgctcgcc aaactcggca ccgaacgcgt cgccctttcc
      601 ggaccgccac tcgactccac ctgcaacgcc atcgtcacca ccagcggcga tcgcgcacac
      661 attctcgttt acgcgtccga tctcgcagac gacacgaaac gccagtcgat cgacgtgtct
      721 gtgagccttc cgcccggcgc tcgcgcgaag caactccacc gcatcgattc aatcgagaac
      781 aacgtgctcg cccgctggca cgaactgggc gccccagcgt atctctcgcg cgaacaaaca
      841 aaggaactcg ccgccgacaa cgcgctgaag ccatcgtctt ctccggtcac catcgagaac
      901 ggccacgcgc agttcacgat ggaaagcccc ggcatcgcgc tgctcgaaat cacactgccg
      961 ccccgcgcct aaagcggagt tatgaacctg gcgcgtcggc tcggcatgcg tcttctgata
     1021 ggggcgtctc tccgagacct tcgtctttcc cgccccgctt tccgataggc acgtgtcatc
     1081 cgacacggct attcgctcgc cgccgccatt cgatcctgcc gcgatcccca atgtcggcgc
     1141 cttccgtttc gactccgcac aaaggcccga gcctgccgag ggcttgcagg cgatttaccg
     1201 cgcaaacctc gcctccccgt cagcctgcgg tgacgagcat gagtttctag atttcttccg
     1261 ttcccctcac cgcatgaaga aactcctttt cgccgccttt ctctgcctcg cttgcagcat
     1321 cggcgtttcc tccgccgcag aaaacgccac tccgcccaac atcgtcctga tcctcatcga
     1381 cgacctgggc tatggcgaca tcggcccgtt cggctcgact aaaaaccgca cgctcgcgct
     1441 cgatcgcatg gcgcgcgaag gccggaagtt caccagttac tacagcgcgc ctgtgtgttc
     1501 accgactcgc gcgcaagtgc tgaccggcgc ctacgcgcaa cgcgtctcca ttccgctcgc
     1561 cgtcatgccc gggcaaaaac acggcctcaa ccccgccgaa aacaccgtcg ccgaactcct
     1621 gaaagcccgc cgctacgcca ccctgtgcat cggcaaatgg cacctcggct acgaaaccga
     1681 attcttcccc acgcaccacg gcttcgatcc ccccggaccc aacgtccgcc ccgccgccca
     1741 cgcggattga actaccgcca caagtcgatt tttaaaccgc cgatgaacac cgctaaacac
     1801 ggattccaaa ccgtcgaatc caacctccct ccgatccgaa tccgtgttca tcggtgtcca
     1861 tccgtggtta atactcaggt gctatttccc ttcgtgtccc ttcgtggttc aactcattcg
     1921 actcatccat cccaacccca ccgcccgcgc ttcgccgatc aggcgaagca cgcaccgtcg
     1981 catccgccct tggacttcag aaaatcagcg caaattagcg gttaaaaaac tccgacaaga
     2041 ttgcctccat tcctattcaa caccgatcca ttcaacccga gataaacacg gattccacgc
     2101 cctcgaatca tccaatcgct tccgttcatc tgaatccgat ccgtgtccca tccgtgatta
     2161 atactcaggt gctattttcc cttcgtgtcc cttcgtggtt caactcattc gactcatcca
     2221 tcccaccctc accgcccgcg cctcgccgat caggcgaagc acgcaccgcc tcatccgcct
     2281 ttcgacttca gaaaatcagc gcaaattagc ggttaaaaaa ctccgactcg caccttctct
     2341 ttctcttcag ccatgaacct ttctttcccc aaacgtttca cctggggcgt cgccgccgcc
     2401 gctcctcaaa tcgaaggcgc cgccttcacc gacggcaaag gcgagtccgt ctgggatcgc
     2461 ttctcgcgca ttcccggcaa ggtcgccaac ggtgacacgc cggacgtcgc gtgcgatcac
     2521 taccatcgct acaaacaaga cttcgcgctc atgcgaaagc tcggcgtaaa acactaccgc
     2581 ctctcgctcg cctggccgcg catcttctcc gaaggccgcg gcgccgtgaa tcagaaaggc
     2641 gtcgatttct acaatcgtct catcgacgcc atgctcgccg ccggcattca accgtgggtc
     2701 acgatgtttc actgggacct gccgcaagcc ctcgaagaca tcggcggctg gcgcgcgcga
     2761 gagacgcccg acgcgtttgg cacctacgcc gacaccatcg tgcgcgccta cggcgatcgc
     2821 gtgaaacact ggatcacgct caacgaaatg cgctgcttca ccgtgctcgc ctatggcgac
     2881 acgatccgcc cgcccggcat cgtcgagccg ccgcaggtcg taaaccaaac ctaccatcac
     2941 gcgcttctcg ctcacgggca cggcgtgcgc gccgtgcggg aacacggcgg ccgcggcgca
     3001 caggtcggca tcacggacga ctccaccatc acggtccccg tcaacgaaac cgaagcggac
     3061 atcgccgcgg cgcgcaccgc cttccaggaa ctcaacattc gctccatcga tccgattttt
     3121 cgcggcggtt attcctccac ctaccttcgc atcaccggca aggatcgcgc caaaacgcag
     3181 cgcggcgact tcgatctcat cagcctgccg actgattttt ttggcttcaa catctacacc
     3241 ggcgtattcg
//
LOCUS       DASPLT010001193         2182 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_199448, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001193 DASPLT010000000
VERSION     DASPLT010001193.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 2182)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2182)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2182
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_199448"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(63..1970)
                     /locus_tag="VEA63_12260"
     CDS             complement(63..1970)
                     /locus_tag="VEA63_12260"
                     /inference="COORDINATES: protein motif:HMM:TIGR03710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HYD84825.1"
                     /translation="MVSPNSTETQGIAAPQAGAPRSLKDVVIRLAGNSQDGIQTIGGF
                     LARLAGRSDQDVMTYMTIPSTISGGPSIFQVRMGTGDILSSGDRADMLVAFYQHSYES
                     HIGSLRPGGVLVYDSDHVKPNTDDHSFAYVAVPITTATVESVGGAGKDKGKNIFVLGL
                     IARIFDLDVAKLSALVKERFGGKAEDVVRNAMLAFDAGYAWPQNNLRDLFYRFDAAEH
                     AAASGRPQVTMDGNMALTYGLLAGGVRHGAGYPITPWSSIMEMLRSELPKYGGIFVQC
                     EDEIAAVSTALGFSYTGRLAITGSSGPGLSLKMEALGWAVMAEMPLIVVNVQRGGPST
                     GMPTNVEQSDLMQAIYGSHGDTPRVVLAPKNVEDCFYIGLEATRIAREYSTPVLILTD
                     QGLSSRIEAFDEPDLASLIVDPQPDLSPRGVEFKPYPLDRITRHAPPGARIESNKYPT
                     VTGLEHDELGHPTGSPALHMKMTAKRREKIKALGASLPAPELDGDNSGEFLLVTWGSS
                     WGPGREALSRIRTAGIKGAHLHLRHIHPLPSGLEETFGRFHKVVVAELNDEGLYGFGQ
                     LAMMLRARYANPAIVSVTKTDGLTFKVSEIVAGVARHLESQALDPAFGSAMHSALSLP
                     TELRTGTPAQS"
BASE COUNT          407 a          675 c          711 g          389 t
ORIGIN      
        1 gggcgcgacg ggagcggcgg cggcaagagc gggggcggcg gaagacatgt tgaaaatgtg
       61 ttttaagatt gggcaggagt tccggtgcgc agttcggtgg gcagcgacag cgcactgtgc
      121 atcgcggagc cgaacgccgg atcgagcgcc tggctctcga ggtgacgggc cacgcccgcc
      181 acgatctcgc tcaccttaaa agtgaggccg tcggtcttcg tcacgctaac gatcgcggga
      241 ttcgcatacc gcgcgcgcag catcatcgcg agctgaccga aaccgtagag gccttcgtcg
      301 ttcagctccg cgaccacgac cttgtggaaa cgtccgaacg tctcttccag accggagggc
      361 aacggatgaa tatggcgcaa atgcaggtgc gcgcccttga tgcctgccgt gcgtatccga
      421 ctgagcgcct cgcgacccgg cccccaactc gagccccacg tcacgagcag aaattcgccc
      481 gagttgtcgc cgtcgagttc cggcgccggc aacgacgcgc cgagcgcttt gattttttcg
      541 cgccgcttcg ccgtcatctt catgtgcagc gccgggctgc cggtcggatg gccaagctcg
      601 tcgtgttcga ggccggtgac ggttggatat ttgttcgact cgatccgcgc gcccggtggc
      661 gcatgccgcg tgatgcgatc gagcggataa ggtttaaact ccacgccgcg cggcgagagg
      721 tccggttgcg gatcgacgat caagctcgcg agatcgggtt cgtcgaacgc ctcgatgcgc
      781 gacgagaggc cttgatccgt gagaatgagc accggcgtgc tgtattcgcg ggcgatgcgc
      841 gtcgcttcga ggccgatgta aaagcagtcc tcgacgtttt tcggcgcaag cacgacgcgc
      901 ggcgtatcgc catgactgcc gtagatcgcc tgcatcaaat cgctctgttc gacattggtc
      961 ggcatgccgg tcgacggtcc gccgcgctgc acgttgacca cgatcagcgg catctccgcc
     1021 atgacagccc aaccgagcgc ttccattttt aacgaaaggc cagggccgga gctgcccgtg
     1081 atcgcgagcc gacccgtgta ggaaaaaccg agcgccgtcg aaaccgccgc gatctcgtct
     1141 tcgcattgca cgaaaatgcc gccgtatttc ggcagctcgc tgcgcagcat ttccatgatc
     1201 gaggaccacg gcgtgatcgg ataacccgcg ccatgccgca cgccgccggc gagcaagccg
     1261 taggtcagtg ccatgttgcc gtccatcgtc acctgcggac ggcccgacgc cgcggcatgc
     1321 tctgcggcat cgaaacgata aaacagatcg cgcagattgt tctgcggcca ggcgtagccg
     1381 gcgtcgaagg ccagcatcgc gttgcgaacg acgtcctccg ccttgccgcc gaaacgctct
     1441 ttcaccagcg cggagagttt cgccacgtcg agatcgaaga tgcgcgcgat caagcccagc
     1501 acgaaaatgt ttttgccctt gtccttgccg gcgccgccga ccgattcgac cgtggccgtc
     1561 gtgatcggca ccgcgacgta ggcgaagctg tggtcgtcgg tgttgggctt cacgtgatcg
     1621 ctgtcgtaaa cgagcacgcc gcccggccgc agcgaaccga tgtgcgactc gtagctgtgt
     1681 tgataaaagg ccacgagcat gtcggcacgg tcgccggaag aaagaatgtc gccggtgccc
     1741 atgcgcactt ggaagatcga cggcccgccc gagatggtgg acggaatcgt catgtaagtc
     1801 atgacgtcct gatcgctccg gccggcgagc cgcgcgagga atcctccgat ggtttggatg
     1861 ccgtcctgcg aattgccggc gaggcggatg acgacgtctt tgagggagcg aggtgcgccg
     1921 gcctgaggtg cggcgatgcc ctgcgtttcg gtagaattcg ggctgaccat gagaaaagaa
     1981 agcgtgcggg gtctctcgtg agcatgacga ccgacacaag cggccgttgc ggggaagccg
     2041 cggacattgg acactcccgc ccgctttggc gaggacttag gcgaaatgcg cgagcgatgc
     2101 gcaacttcgc cgccggcacc gggcagcgac cgccgccacg gctacagcgg ttcaaaaatt
     2161 ccatagccaa atttgtgggc cg
//
LOCUS       DASPLT010001194         1068 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_14905, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001194 DASPLT010000000
VERSION     DASPLT010001194.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1068)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1068)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1068
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_14905"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..218)
                     /locus_tag="VEA63_12265"
     CDS             complement(<1..218)
                     /locus_tag="VEA63_12265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD84826.1"
                     /translation="MRNETDSIRANGVRSMSAGRKFAGGSGRIASAGGRRTAVRTAPH
                     TPVKAAHALTATATTSTFFASTNSNSGKR"
     gene            217..>1068
                     /locus_tag="VEA63_12270"
     CDS             217..>1068
                     /locus_tag="VEA63_12270"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HYD84827.1"
                     /translation="MALRNIERRPSRAILTVVALALATGILIVPSALRDGIDYVLDYQ
                     WDLIQRQTAFVSLIEPGPARAIADFKHLPGVILAEPVHTVPVELRFGHRQRRLALTGL
                     DANAELNRIIDEDGRRLTLPPNGLVLSVKLAAVLGVRPGDWLNVRVLEGERAEIVLPL
                     AAVAEDFAGTAAFVELDALNRLLGEGDRINGAYLTVAAGAWPEFLQALKETPRARSVV
                     IKEGIRASFRKTTAESMGVIQKIYMTFAIVVAFGIVYNSARISLSERQRELATLRVVG
                     FTRGEVAA"
BASE COUNT          159 a          311 c          396 g          202 t
ORIGIN      
        1 cgttttccgg agttggagtt cgtgctcgcg aaaaacgtgc tggtcgtggc ggtcgcggtg
       61 agcgcgtgcg ccgctttcac cggggtgtgg ggcgcggtgc gcacggcggt gcgcctgccg
      121 ccagcggagg cgatgcggcc ggagccgccg gcgaattttc ggccggcgct catcgaacgc
      181 acgccgttcg cgcgaatgct atccgtttcg ttgcgcatgg cgctgcgcaa tatcgagcgg
      241 cgtccgtcgc gcgcgatcct caccgttgtg gcgctcgcgc tggcgacggg aattctcatc
      301 gtgccgagcg cgcttcgcga tggcatcgat tatgtgctcg attaccaatg ggacctgatt
      361 cagcggcaga ccgcgttcgt gtcgctgatc gagccggggc cggctcgcgc gatcgcggat
      421 tttaagcatt tgcccggcgt gattctcgcg gagccggtgc acaccgtgcc ggtggaactg
      481 cgattcggcc atcgccagcg gcggcttgcc ctcacggggc tcgacgcgaa cgcggagttg
      541 aaccgcatca tcgacgaaga cgggcgtcgg ttaaccctgc cgccaaacgg tctcgtgctg
      601 tcggtgaaac tcgcagcggt gctcggcgtg cggccgggag actggttgaa cgtgcgtgtg
      661 ctcgagggcg aacgcgcgga gatcgtgctg ccgctggccg cggtcgcgga ggatttcgcg
      721 ggcacggcgg cattcgtcga actcgacgcg ctgaatcgcc tgctcggcga gggcgatcga
      781 atcaacggcg cttatctcac cgtggcggcg ggcgcatggc cggagttttt gcaggcgttg
      841 aaggagacgc cgcgcgcgcg gagtgtggtg atcaaggaag gcatccgcgc cagctttcgg
      901 aaaacgacgg cggaaagcat gggcgtgatt cagaaaatct acatgacctt cgcgatcgtg
      961 gtggcgtttg gcatcgttta taacagcgcg cggatctcgc tgtcggagcg gcagcgcgag
     1021 cttgcgacgc tgcgagtggt gggcttcacg cgcggcgagg tggcggct
//
LOCUS       DASPLT010001195         1458 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_138068, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001195 DASPLT010000000
VERSION     DASPLT010001195.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1458)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1458)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1458
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_138068"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1458
                     /locus_tag="VEA63_12275"
     CDS             <1..>1458
                     /locus_tag="VEA63_12275"
                     /inference="COORDINATES: protein motif:HMM:NF014850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HYD84828.1"
                     /translation="HSASCEIVIRLDNRNDPEVPPGKPFAELDFCWFGGLYRGAELRV
                     RPPLHITEAASAGEPAGGGVFVRTLSASPDTARVAVKTHLRNLAPDDRVVRVQIDLHR
                     AGVLIVSNTSAPHRVPAASSLHVEQEFLVNTPSLWSPASPSLYDVTVRVIADDGAELD
                     RRGERLGIRHIAFSRSGGFMINGQRVRLRGTNRHQEMPRVGYAVPRAAHYRDARRIKD
                     AGFDYVRLSHYPQSPDFLDACDELGIVVMNCIPGWQFMGGDVFRAACFAAARDLIRRD
                     RNHPCVVLWELSLNETPMDEAFMATLHRIGHEEYPGDQMFTCGWIDAYDVFNHSRQHG
                     SIHTWQNGDKALVIAEYGDWEFYAANEGFDQKTGAGVFDRWSTARQFRRDGERGLLQQ
                     ALNHIIALNDTLSSPAVLDGQWAMFDYTRGYDPVRASVGVMDVFRLPKFSYHFYRSQR
                     DSDETGPGYSGRPVVFIASFWTPTSDLRIRVFSNC"
BASE COUNT          250 a          533 c          404 g          271 t
ORIGIN      
        1 aacacagcgc gtcctgcgaa atcgtcattc gtctcgacaa ccgcaacgat cccgaagtcc
       61 cgcccggaaa accgttcgcc gaactcgact tctgctggtt cggcggactc taccgcggcg
      121 ccgaactccg cgtgcgcccg ccgctccata tcaccgaagc cgcctccgct ggcgaaccgg
      181 cgggcggcgg cgtctttgtg cgcacgctca gcgcctcgcc cgacaccgca cgcgtcgccg
      241 tcaaaactca cctccgcaat cttgcgcccg acgatcgggt cgttcgcgtt caaatcgatc
      301 ttcatcgggc gggggtgctc atcgtttcca acacctcagc cccgcaccgc gttcccgccg
      361 cttcctccct ccacgtcgag caagagttcc tcgtcaacac gccctcactc tggagccccg
      421 cgtctccgag cctctatgac gtcaccgtgc gcgtaatcgc cgatgacggc gccgagctcg
      481 atcgtcgcgg cgaacgtctt ggcatccgcc acatcgcgtt ttcccgctcc ggcggtttca
      541 tgatcaatgg ccaacgcgtg cgactgcgcg gcaccaaccg tcaccaggaa atgccccgcg
      601 tcggttacgc cgttccgcgc gccgcgcact atcgcgatgc gcgccgcatc aaggacgcgg
      661 gcttcgacta cgtgcggctc tcgcactacc cgcagtcgcc cgacttcctc gatgcgtgcg
      721 acgagctcgg catcgtggtg atgaactgca ttcccggctg gcagttcatg ggcggcgacg
      781 tttttcgcgc cgcgtgcttc gccgccgcgc gcgatctcat ccgccgcgac cgcaatcacc
      841 cgtgcgtcgt gctctgggaa ttatcgttga acgaaacgcc gatggacgag gcgttcatgg
      901 ccacgctcca ccgcatcggg cacgaggaat atcccggcga ccagatgttc acctgcggct
      961 ggatcgacgc gtatgatgta ttcaatcatt cgcgacaaca cggcagcatc cacacctggc
     1021 aaaacggcga caaggcgctc gtcatcgccg aatacggcga ttgggaattc tacgccgcca
     1081 acgagggctt cgatcaaaaa accggcgccg gtgtttttga tcgctggagc accgcgcgcc
     1141 aattccgccg cgacggcgaa cgcggcctcc tccagcaggc cctgaaccat atcatcgcgc
     1201 tcaacgacac gctcagctcg cccgcggtgc tcgacggcca gtgggcgatg ttcgattaca
     1261 cccgcggcta cgatcccgtg cgcgcgtcgg tcggcgtgat ggacgtcttt cgcctgccga
     1321 agttttccta ccacttctat cgcagtcagc gcgattccga cgaaaccggt ccaggctaca
     1381 gcggcagacc cgtcgtcttt atcgcgtcgt tttggacccc gacgtccgac ctgcgcatcc
     1441 gcgtcttcag caactgcg
//
LOCUS       DASPLT010001196         1629 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_280164, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001196 DASPLT010000000
VERSION     DASPLT010001196.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1629)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1629)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1629
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_280164"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            207..>1629
                     /locus_tag="VEA63_12280"
     CDS             207..>1629
                     /locus_tag="VEA63_12280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD84829.1"
                     /translation="MIPSVRPRLLLSALVLSSVATAIAAEKPKIAVFSGPTATIQSNQ
                     ALITSNKARQKYGLPLRTGADGQPAVDVLRPQRLAAPAKVYIEAFSAHPLEADVAEMY
                     GAPDGYLDAQGNFSKEKKNPSDKAVYEAVLDPSDGLYPLPYMALQVDGQPWDANGPKP
                     DMGEEGARQHHYPDGSRFFEEMERLGGKVFEKADFDFYRAAPSGGYTKGLPAALRTDV
                     GEGDIPPEILGVDYFPYGYPPPRAYLAKATNVVQRAMATGKYTGGMWLEGSPGIEDTS
                     YWLNLLVDTDRPMTTNSSQRDRGNISPDGDANMIASIRYITSDVWADDKGADRLGMVV
                     HQDSLIYASREIMKGDARPGGYVATGGDGGLVGNMVGAPVVSYVPNRKHTSTSDVRVT
                     QLPASTTGVKRVDDKLVSVPVPIKNAAGELLPSAIPNVTFVKGARWSSLDNTEEPASE
                     VDILARLEADLLEEPLSGMVAEGT"
BASE COUNT          321 a          615 c          432 g          261 t
ORIGIN      
        1 tggagcctgc gattggacat cttcaatctc accaacgagc gctccttccg ctccatgaaa
       61 ctcgcgagcg gcgaggtgct cgccatcgcg aatcccgacc gccgctggca gctcacgctc
      121 cgcaccacgt tttaagtcac cgccccgctg tatccatttc acccacacac gcccatcgca
      181 ttccccgcct agaactcacc tcaaccatga tcccatccgt tcgcccacgc ctgttgttgt
      241 ccgctctcgt tctttcctcc gtcgccaccg cgatcgccgc cgaaaaaccg aagatcgccg
      301 tcttctccgg ccccaccgcc accatccaga gcaatcaggc gctgatcacc tccaacaagg
      361 cgcgccagaa atacggcctg ccgctccgca ccggcgccga cggccagccc gccgtcgacg
      421 tcctccgccc gcaacgcctc gccgccccgg cgaaggttta catcgaagcg ttcagcgctc
      481 acccgctcga agccgacgtc gccgaaatgt atggcgcgcc cgacggctat ctcgacgccc
      541 agggcaactt ctccaaggaa aagaaaaatc cttccgataa agccgtctac gaagccgtcc
      601 tcgatccctc cgacggactt tatccgctgc cctacatggc gctccaagtc gacggtcagc
      661 cttgggacgc caacggcccg aaacccgaca tgggcgaaga gggcgcgcgc cagcatcatt
      721 acccggacgg ctcgcgtttc ttcgaggaga tggaacggct cggcggcaag gttttcgaaa
      781 aggccgactt cgacttctac cgcgccgcgc cttccggcgg ttacaccaaa ggcctgcccg
      841 ccgcgctgcg caccgatgtc ggcgaaggcg atatcccgcc ggaaatactc ggcgtcgatt
      901 attttcccta tggttatccg ccgcctcgcg cctacctcgc gaaagcgacg aacgtcgtcc
      961 aacgcgccat ggccaccggc aaatacaccg gcggcatgtg gctcgagggt tcacccggca
     1021 tcgaggacac cagctactgg ctgaatctcc tcgtcgatac cgaccggccg atgaccacga
     1081 attcctccca gcgtgaccgc ggtaacatca gtcccgacgg tgatgccaac atgatcgcgt
     1141 cgatccgcta catcacctcg gacgtgtggg ccgacgacaa aggcgccgac cggctcggca
     1201 tggtcgtcca ccaggacagc ctcatctacg cctcgcgcga aatcatgaaa ggcgacgcgc
     1261 gtccaggtgg ctacgtcgcc accggcggcg acggcggcct cgtgggcaac atggtcggcg
     1321 cgcccgtcgt ctcctacgtg ccgaaccgca agcacacctc cacgtccgac gtgcgcgtga
     1381 ctcagctccc cgcgagcaca accggcgtga aacgcgtcga cgacaaactc gtcagcgtgc
     1441 ccgtcccgat caaaaacgcc gccggcgaac tgctgccatc cgcgatcccc aacgtcacgt
     1501 tcgtcaaagg cgcacgctgg agttcgctcg acaacaccga ggagccggcg agcgaggtcg
     1561 acatcctcgc gcgactcgaa gccgatctcc tcgaagaacc gctctccggc atggtcgccg
     1621 agggcacgc
//
LOCUS       DASPLT010001197         2518 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_95393, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001197 DASPLT010000000
VERSION     DASPLT010001197.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 2518)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2518)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2518
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_95393"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(37..>2518)
                     /locus_tag="VEA63_12285"
     CDS             complement(37..>2518)
                     /locus_tag="VEA63_12285"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HYD84830.1"
                     /translation="GVLWIGTVNVGVIRYKDGAFSALGKRDGLASDRVLSLAESDDGS
                     LWVGTEDGLSQLSDVKFPILSSSEGLPHEAALAVATGAEGDVWVGTPDGLSHYRDGKF
                     ANYGVGGADGFTSRWIKRLYVARDNDVYVIGARKNIDRFSGGRVVKSWANAVWPKAIA
                     EDSRGLLMAIEGRLMRLEGDELQPFLLADGTEVSLRWITDMLVARDDSIWIAATEGVF
                     QIVDGVIYNRCHLNGVYHTAFYYLCETADGAVWAAQNTGIARFKDGRLALFTKQQGLH
                     ENFVYAMMPDRLGYIWMDSTRGIFRVSERELNAVADGLAERVSCDVFDGEDSVKTTDK
                     SAQEYSAGRTPDGRIWFPSSKGVIVIDPANVPLDARPPHVSIERVLINGREYSPDRVP
                     TLEPTAGNLEFHYAALNYAAPLKTRYRYRLEGYETAWVDAGGRRSAFYTNLAHGRYRF
                     HVQACNADGVWNTTGDAFALVLPARFYETLWFRVAWAAGAIALLGYIWWMMHLRRKHQ
                     QLRQTHDLLEAKVQERTAELRAEIEEREKVQKEVERVHRELLEISRQAGMAEVATGVL
                     HNVGNVLTSLNVSATLLHDRIRESKVSCVAQLRDLLRGHAADLGAFLVKDPRGRQVPD
                     FVTTLADHLATEQAELMRELESLRKNVEHIRDIVAMQQNYARVSGVTERVAVQSLVED
                     ALRMNEVSLARHAVELVRDYRADPVITVEKHKVLQILVNLLRNAKYACEESGRDDRRI
                     VVRISAEGGGVQITVSDNGVGISRENLTRIFSHGFTTKKSGHGFGLHNGALGAKNIGG
                     SLTVNSGGAGKGATFVLELPEEPPAVGV"
BASE COUNT          456 a          971 c          632 g          459 t
ORIGIN      
        1 ccgcgcctgc ctgcttttga actccgtccg cctctctcaa acccccaccg ccggcggctc
       61 ctccggcagc tcgagcacaa acgtcgcccc cttcccggct ccgccgctgt tcacggtcag
      121 cgatcctccg atgttcttcg cgccgagcgc gccattgtgc agcccaaagc cgtggcccga
      181 tttcttcgtc gtgaaaccgt gcgagaagat ccgcgtcagg ttctcccgcg atatccccac
      241 gccattgtcg ctcacggtga tttgcacgcc tccaccctcc gccgaaattc gcaccacgat
      301 ccggcgatcg tcgcgacccg actcctcaca cgcgtatttc gcgttgcgca acaggttcac
      361 caggatttgc agcactttgt gcttctccac tgtgatgacc ggatcggctc gatagtcgcg
      421 aaccagctcc accgcgtggc gtgcgagcga cacctcgttc atcctcaacg catcctcgac
      481 gaggctctgc accgccaccc gctccgtcac gcccgagact ctcgcgtagt tctgctgcat
      541 cgcgacgatg tcgcggatgt gctcgacgtt cttccgcaac gattcgagct ccctcatcaa
      601 ctccgcctgc tccgtcgcga gatgatcggc gagcgtcgtc acgaaatccg gcacctgccg
      661 cccgcgtgga tccttcacta aaaacgcgcc gagatccgcc gcgtgcccgc gcagcaggtc
      721 ccgcaactgc gccacacaac tgaccttcga ctcgcgaatc cggtcgtgaa gcaaggtcgc
      781 ggaaacattc aggctcgtca gcacgttgcc cacattgtgc agcacgccgg tcgccacttc
      841 cgccatgccc gcctgccgcg agatttccaa cagctcgcga tgcacacgct ccacttcctt
      901 ctgcactttc tcgcgctcct cgatctccgc ccgcagctcg gccgtgcgct cctgcacctt
      961 cgcctcgagc aggtcgtgcg tctgccgcag ctgctgatgc ttccgccgca agtgcatcat
     1021 ccaccagata tacccgagca acgcgatcgc cccggccgcc cacgcgacgc gaaaccacag
     1081 cgtctcgtag aatcgcgccg gcaacaccaa cgcaaacgcg tcgcccgtcg tattccatac
     1141 cccgtcggcg ttgcacgcct gcacgtgaaa acgataccgg ccgtgcgcga gattcgtgta
     1201 gaacgccgac cggcgtccgc ccgcgtcgac ccatgcggtc tcgtagcctt cgagccggta
     1261 gcgataccgc gttttcaacg gcgccgcgta gttcaacgcc gcataatgaa actccagatt
     1321 gcccgccgtc ggctcgagcg tcggcacccg atccggcgaa tactcccggc cattgatcaa
     1381 cacccgctcg atcgacacat gcggcggccg cgcatccagc ggcacgttcg ccggatcgat
     1441 gacgatcacg cctttcgaac tcggaaacca gattcgcccg tccggcgtgc ggcccgccga
     1501 atactcctgc gcgctcttgt cggtcgtttt caccgaatcc tcgccgtcga acacgtcgca
     1561 gctcacccgc tcggccagac catccgcgac cgcgttcaat tcgcgttcgc tcacgcggaa
     1621 aatcccccgg gtcgaatcca tccagatgta accgagccga tccggcatca tcgcatacac
     1681 gaaattctcg tgcagcccct gctgtttcgt gaacaacgcc agccggccgt ccttgaaccg
     1741 cgcgataccc gtgttctgcg ccgcccacac cgcaccatcg gccgtttcgc acagataata
     1801 gaaggccgtg tggtaaacgc cgttcaagtg acagcggtta taaatcacgc cgtcgacgat
     1861 ttgaaaaacg ccctcggtcg ccgcgatcca gatcgagtca tcccgcgcca ccagcatgtc
     1921 cgtgatccag cgcagcgaaa cctccgtgcc gtccgccagt aaaaacggct gcagctcgtc
     1981 gccctcgagc cgcatcaacc gcccttcgat ggccatcagc aatcctcgcg aatcttccgc
     2041 gatcgctttc ggccacaccg cgttggccca gctcttcacc acccgcccgc cggagaagcg
     2101 atcgatgttt ttccgcgcgc cgatgacata cacgtcgttg tcgcgcgcca catagagccg
     2161 cttgatccag cgcgacgtga aaccgtccgc cccgccgaca ccgtagttcg caaattttcc
     2221 gtcgcgataa tgcgagagcc cgtccggcgt gcccacccac acatcgccct ccgcccccgt
     2281 cgccaccgcg agggctgcct cgtgcggcaa accttccgac gacgatagaa tcgggaactt
     2341 cacgtcggac aactggctca acccatcctc cgttcccacc cagaggctgc cgtcgtcgct
     2401 ctccgcgagc gacagcacgc gatcgctcgc cagcccgtcg cgtttgccca gcgcgctgaa
     2461 agcgccatcc ttgtagcgaa tcacgcccac gttcaccgtc ccgatccaca gcacaccg
//
LOCUS       DASPLT010001198         1007 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_53880, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001198 DASPLT010000000
VERSION     DASPLT010001198.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1007)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1007)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1007
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_53880"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..581
                     /locus_tag="VEA63_12290"
     CDS             <1..581
                     /locus_tag="VEA63_12290"
                     /inference="COORDINATES: protein motif:HMM:NF019113.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HYD84831.1"
                     /translation="GRGNGWVIFSLSELLAVLPADHELRDELLAMFRELSEGYLAQQG
                     ATGMWHQVLNEHDSYPETSCTSMFAYAFARGVQYGWFQDNAPYLRAVFKAWEALNRTS
                     IDRSGNIYGVCRGSEFSFTSEYYKRDLLWNLNDTHGIGIVLLAGVEVLRLTKFLQKNE
                     GGVDGVAMEQMPRRRVPGARRHSHSKVKAAAK"
     gene            complement(685..>1007)
                     /locus_tag="VEA63_12295"
     CDS             complement(685..>1007)
                     /locus_tag="VEA63_12295"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HYD84832.1"
                     /translation="VGSPNLQVIYDPVNLLSAKNHARQKEITDEATSLLSERICVVHA
                     KDFTVHGDEIREHPAGDGSFDYAGLLGWVKQQKPGIDILLENTHPDSIERTIAFVREA
                     YARA"
BASE COUNT          177 a          293 c          333 g          204 t
ORIGIN      
        1 gggggcgggg caacggctgg gtcatcttct cgctttccga gctgctcgcg gtgttgccgg
       61 cggatcatga gctgcgcgac gagctgcttg cgatgtttcg cgagcttagc gaaggctatc
      121 tcgcgcaaca gggggccacc ggcatgtggc atcaggtatt gaacgagcat gactcgtatc
      181 cagaaacctc ctgcacctcc atgttcgcgt atgccttcgc gcgcggcgtg caatacggct
      241 ggttccagga taacgcgcct tacctccgcg cagtcttcaa agcgtgggag gcgctgaacc
      301 gcacctcgat cgatcgctcg ggaaatatct acggcgtctg ccgcgggtcg gagttctcgt
      361 tcacctcgga gtattacaag cgcgacctgc tgtggaacct caacgacacg cacggcatcg
      421 ggattgtgct gctcgcgggc gtggaagtgc tgcggctgac gaaattcttg cagaagaatg
      481 aaggtggcgt ggatggcgtc gcgatggagc agatgccgcg acgccgggtt ccgggagcgc
      541 gccgtcattc ccattccaag gtcaaggccg cggcgaagta ggcggaatcc gacgccaaag
      601 gtggggcgaa ccgtcctcgg tgagccgtga tccgtggcgg cggctcagcg tgacacttcg
      661 ccctaccgga cccgacaaac accgtcacgc gcgtgcgtag gcttcgcgca cgaaggcgat
      721 cgtgcgctcg atcgagtccg gatgcgtgtt ttcgagcagg atgtcgatgc cgggcttctg
      781 ttgtttcacc cacccgagaa ggcctgcgta gtcgaatgag ccgtcgccgg cgggatgctc
      841 gcggatttca tcaccgtgca ccgtaaaatc cttcgcgtgc acgacgcaga tccgctcgct
      901 cagcaggctg gtcgcctcgt ccgtgatttc tttttggcgc gcgtgattct tagccgagag
      961 caggtttacg gggtcgtaga tgacctggag attcggagag ccgacgt
//
LOCUS       DASPLT010001199         1366 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_218686, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001199 DASPLT010000000
VERSION     DASPLT010001199.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1366)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1366)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1366
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_218686"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(280..1107)
                     /locus_tag="VEA63_12300"
     CDS             complement(280..1107)
                     /locus_tag="VEA63_12300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009508725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase assembly protein"
                     /protein_id="HYD84833.1"
                     /translation="MIDWGHWHNEPYLVGGLVLAGWLWAILAGPLRARLARRAGLPAD
                     IPFPRGHAWRFYSALVIFYLAVGSPLDQIGERFLFSVHMLQHQLLVYPAAILFLLGLP
                     WWMVDAAFTRPVGNPPPPPALSLRILTNPVVCGVVYTLVISVWHMPDLYDLALRNKVV
                     HVLEHLMFFGAALFYWWPMLSPSRALPPISYAAQILYFFAVLVGMTPIFAYITFSHEI
                     LYPTYEYAPRILADFSPADDQLLAGASMKLVGMLVALAAVAVSFFRWYQAGQKPGQN"
     gene            complement(1138..>1366)
                     /locus_tag="VEA63_12305"
     CDS             complement(1138..>1366)
                     /locus_tag="VEA63_12305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD84834.1"
                     /translation="PTAIQLSNAPRREHLAAYFHPIFKTHFLSPNPTLRRRKVTNPFT
                     QSDRGPSRANHESITAAPAPASFSRPAIFLV"
BASE COUNT          301 a          361 c          492 g          212 t
ORIGIN      
        1 tggcgaagcg acccgagaac aagggcaagc agatcgtcgc gatcattccg tcgagcacgg
       61 aacgttatct ctccacgtgg ttgttcgccg atgtgaacac cgagagcgac tcgatcgacg
      121 acctcgtgcc ctccggcgcc acgagctgaa cgcgcggagc agcgaaccat ttgggttgaa
      181 cagggccggt cttggaccgg ccctttttta tggggcgcgc caacagagcg tgccggccgg
      241 ccgatatgag gacgaaacgg aagcgtccta tcagcggcgt cagttttgtc cgggcttttg
      301 cccggcttga taccagcgga aaaaactgac ggcgacggcg gcgagcgcga ccagcatgcc
      361 gacgagtttc atgctcgcgc cggcgagcag ttgatcgtcg gccggcgaga aatcggcgag
      421 gatccgcggc gcgtattcat aagttggata gaggatctcg tgggaaaatg tgatgtaggc
      481 gaagatcggc gtcatgccga cgagcacggc aaaaaagtag aggatctggg cggcgtagct
      541 gatcggtggc aatgcgcggg acgggctgag catcggccac cagtagaaga gcgcggcgcc
      601 gaaaaacatc agatgctcga gcacgtggac gaccttgtta cgcagcgcga gatcgtagag
      661 gtcgggcatg tgccagacgg aaatcaccag cgtgtaaacg acgccacaga cgaccgggtt
      721 cgtcaggatg cggagcgaca aggcgggtgg cggcggcgga tttccgacgg gtcgggtgaa
      781 cgcggcgtcg accatccacc agggcaggcc gagcagaaac agaatcgcgg ccggatacac
      841 gagcaactgg tgttgcagca tgtgaacgct gaagagaaag cgctcgccga tctggtcgag
      901 gggtgaaccg accgcgaggt agaagatgac gagcgccgag tagaaccgcc acgcatggcc
      961 gcgcggaaac ggtatgtcgg ccggcaggcc ggcgcggcgg gcgagtcggg cgcgaagcgg
     1021 tccggcgaga atcgcccaca gccagcccgc gagcacgagc ccgccgacga gataaggttc
     1081 attgtgccag tgcccccagt cgatcatgat gagaccacag catagaggtg aacgcggtca
     1141 gacgaggaat atcgctggcc ggctgaacga agccggcgcg ggcgctgcgg tgatcgattc
     1201 gtgatttgcg cgcgacggtc cgcggtcaga ctgcgtaaac ggatttgtga ccttgcggcg
     1261 gcgaagggtc gggtttgggc tgaggaaatg tgttttgaaa atcgggtgaa agtatgccgc
     1321 gaggtgttcg cgacgaggag cgttcgacag ctgaatggcg gtcggc
//
LOCUS       DASPLT010001200         1687 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_95420, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001200 DASPLT010000000
VERSION     DASPLT010001200.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1687)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1687)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1687
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_95420"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..485
                     /locus_tag="VEA63_12310"
     CDS             <1..485
                     /locus_tag="VEA63_12310"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HYD84835.1"
                     /translation="TRATSVTKRLQPDRATASDIDFGDFDALLGYHLRRAHGAMLRGF
                     SGAVAEFDLTQKQSAILWLIQLNPGVSQVAVASAFGMDRPTMMALTDRLELRGLLTRV
                     RSTVDRRCQELHLTLSGRSLLAKLKKRVARHEQRIRALFTPAEYDILVSALRKLQEHA
                     "
     gene            542..1408
                     /locus_tag="VEA63_12315"
     CDS             542..1408
                     /locus_tag="VEA63_12315"
                     /EC_number="4.1.2.61"
                     /inference="COORDINATES: protein motif:HMM:NF006588.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxycinnamoyl CoA hydratase/lyase"
                     /protein_id="HYD84836.1"
                     /translation="MSTDNNNNPWGNTVDVEFSDDIAFVYFNRPDKKNCMSPTLNREM
                     FATLHALELDDRCKVVVLTGRGAAWSAGMDLKEYFRETDNEGDAFKMRIREESAAWQQ
                     WKLRFHAKPTIAMVNGWVFGGAFTPLTSCDLAIAADDAQFGLSEINWGIIPGGNVTKA
                     VADTMGQRNALYYIMTGETFDGKKAAAMGLVNESVPRDQLEKRVRELAKVLMAKDPVV
                     LRAAKEVFKRVRFMDWETSNDYIYSKTDAANLRSGAEFRQGAMHAFLDEKKLKPGLAT
                     YQKEDGNKGGKN"
     gene            1409..>1687
                     /locus_tag="VEA63_12320"
     CDS             1409..>1687
                     /locus_tag="VEA63_12320"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HYD84837.1"
                     /translation="MTAPVLIAGAGIGGLSMALTLHQIGVPCRVFESVANVKALGVGI
                     NLQPNAVRELYELGFGPDALDTIGIQTREWALVGLNGHDVYTEPRGALA"
BASE COUNT          351 a          538 c          507 g          291 t
ORIGIN      
        1 tgacccgcgc cacttccgtt acaaaacgcc tccagcccga ccgggccacg gcttccgaca
       61 tcgacttcgg cgacttcgac gcgttgctcg gttaccacct gcgccgcgcg catggcgcga
      121 tgttgcgcgg tttcagcggc gccgtcgccg agttcgacct cacgcagaag caaagcgcga
      181 tcctgtggct gatccagctg aatcccggcg tgtcgcaggt tgctgtcgcg agcgcgttcg
      241 gcatggaccg tccgacgatg atggcgctga ccgatcgcct cgagttgcgc ggactgctga
      301 cacgcgtgcg gtccaccgtc gatcgccgct gccaggaatt gcacctgacg ctcagcggca
      361 gaagcctgct cgcaaagctg aagaagcgcg ttgcacggca cgagcagcgc atccgcgcac
      421 tgttcacgcc tgccgaatac gacatcctcg tttccgcgct gcggaaactg caggaacacg
      481 cctgagcttt tgtcactcca tacatactta ctttcctttt ccctccaacc tggaacctac
      541 gatgagcact gataacaaca acaacccctg gggcaacacc gtcgacgtcg aattctccga
      601 tgacatcgcg ttcgtctatt tcaaccgtcc ggacaagaag aactgcatga gcccgacgct
      661 gaaccgcgaa atgttcgcga cgctgcatgc gctagaactc gacgaccgct gcaaggtcgt
      721 cgtgcttacc ggtcgcggcg cggcatggtc ggccggcatg gacctgaagg aatatttccg
      781 cgaaaccgac aacgaaggcg atgcattcaa gatgcgcatt cgcgaagagt ccgccgcgtg
      841 gcagcagtgg aaactacgct tccatgcgaa gcccacgatc gcgatggtga acggctgggt
      901 gttcggtggc gcgttcacgc cgctcacgtc gtgcgacctc gcgattgcgg ccgacgacgc
      961 gcagttcggt ctgtccgaaa tcaactgggg catcattccc ggcggcaacg tgacgaaggc
     1021 cgtggccgac acgatgggcc agcgcaacgc cctgtactac atcatgaccg gtgaaacctt
     1081 cgacggtaaa aaagccgcgg caatgggcct cgtcaacgaa tcggtgccgc gcgaccagct
     1141 cgagaagcgc gtgcgcgaac tcgcgaaggt attgatggcg aaagatccgg tggtgctgcg
     1201 cgcagcgaag gaagtattca agcgcgtgcg tttcatggac tgggaaacgt cgaacgacta
     1261 catctactcg aaaaccgatg ccgcgaacct gcgcagcggc gccgagtttc gccagggcgc
     1321 gatgcatgcc ttcctcgacg agaagaaact gaagccaggt ctcgcgacct accagaaaga
     1381 agacggcaat aaaggtggca agaactgaat gactgctcca gtcctgatcg cgggcgccgg
     1441 catcggaggc ctcagcatgg cgctcacgct gcaccagatc ggcgtgccgt gccgcgtgtt
     1501 cgaatccgtt gcgaacgtga aagcgctcgg cgtcggcatc aacctgcagc cgaacgcggt
     1561 gcgtgaactg tacgagctcg gcttcggtcc cgatgcgctc gacacgatcg gcatccagac
     1621 gcgcgaatgg gcgctcgtcg gcctcaatgg ccacgacgtg tacaccgagc cgcgcggcgc
     1681 actcgcc
//
LOCUS       DASPLT010001201         1170 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_218689, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001201 DASPLT010000000
VERSION     DASPLT010001201.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1170)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1170)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1170
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_218689"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(125..790)
                     /locus_tag="VEA63_12325"
     CDS             complement(125..790)
                     /locus_tag="VEA63_12325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD84838.1"
                     /translation="MNTFDELVATTFAHGVNALCWQRVLPGDFSEVVAQLGRGEGIVP
                     LDDTRLRSLSVSPAGKAAIDVLLSDLRMLRDRGLSPELNCIYAYPRDEEPGAVRTDVF
                     SFHADSATVETDTYLCTYHGASSEGLRNDEARRHIEIPETRAALLQEFGGGDDDAFRE
                     YLSDACYDLHYAALPHAQPYSFGLFNLWRIAVDYPGSPVPPCIHRAPSTSDGDPPRLL
                     LIS"
BASE COUNT          238 a          328 c          406 g          198 t
ORIGIN      
        1 gaggggcaga agacgtttca tggggcgagg ggaagcggag cgttgatcgc gtgcagccga
       61 gggcaaggcg agatcgcggc atgcgccaac gggaatgaag gcagcccggg gctgcgcacg
      121 atcgtcaact gatgagcagc agccgcggcg gatcgccgtc ggaggtggac ggagcgcgat
      181 ggatgcacgg cggcaccgga ctgccggggt aatcgacggc gatgcgccag agattgaaca
      241 ggccgaacga gtagggctgc gcgtggggca gtgcggcgta gtgcagatcg tagcacgcgt
      301 cgcttagata ttcgcgaaac gcgtcgtcgt cgccgccgcc gaattcttgg aggagcgcgg
      361 cgcgtgtttc aggaatttcg atgtggcgac gcgcctcgtc gttgcggagg ccttcactcg
      421 atgcgccgtg atacgtgcac aaataggtgt ccgtttccac cgtcgcgctg tcggcgtgaa
      481 acgagaagac gtccgtgcgc acggctcctg gctcttcgtc gcggggatag gcgtaaatgc
      541 agttaagctc gggcgagagt ccgcggtcgc ggagcatccg tagatcggaa agcaacacgt
      601 cgatcgcagc tttgcccgca gggctcacgg agagcgaacg cagccgcgtg tcatcgagcg
      661 gcacgattcc ttcgccccga ccgagctgcg cgacgacctc gctgaaatcg ccgggcagca
      721 cgcgctgcca gcaaagcgcg tttacaccgt gggcgaacgt cgtcgcgacg agttcgtcaa
      781 acgtattcac atatcggatg cgttcgtagg ccacggaacc tttcgagaag cttcgggtag
      841 acggagtaaa gggatcgacc ggcgtcatga gcgacgcaaa ggaggcgatg gaaccgagga
      901 agatgtcacg ttttaagtca cgctcggttc gaaaagcgcg ggaagtagtg tcacgcaatg
      961 cgtgacactg gatcgaaaca aaacccggcg gcgccgccgg tttcagttcc cgcctcgggc
     1021 ctcaaagatt ctccgggtgc tcgggcttgc ggtgatgaag gacaacccag tttccgtccg
     1081 ggtcggcgac cacgaccatc caacatatcg gggtttcgcg tggttcccag atgaacgtca
     1141 cgccgctcgc gcgcagcttt gcaacgtagc
//
LOCUS       DASPLT010001202         1617 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_260027, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001202 DASPLT010000000
VERSION     DASPLT010001202.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1617)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1617)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1617
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_260027"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..863)
                     /locus_tag="VEA63_12330"
     CDS             complement(<1..863)
                     /locus_tag="VEA63_12330"
                     /inference="COORDINATES: protein motif:HMM:NF038287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Amuc_1100 family pilus-like protein"
                     /protein_id="HYD84839.1"
                     /translation="MLAAIVLIAVGEGWWWRERVEAGRRAEEKLARLRDEEKTLAAAT
                     AADFDAMADLADARAELAVLNLRVADRLERGEVPRQRAEAYFDLATFAARMRTAAAQH
                     GVELAPDEHFGFAEYAGKGPAPALIDAVFLDRLRVEYLLRTLFASRPSRLVSVQRERV
                     ETEGAERQRGADDTFEFASTRSVRVPGLLAGRAFKLTFIAGTASVRALLDGFTVSDQP
                     ITVRSVEAARIDGAEIRSLANGHSEEPRLLVAGGDSRFTVTVEWVAAERPLAFAEETT
                     ADSRGQSERSA"
     gene            complement(902..>1617)
                     /locus_tag="VEA63_12335"
     CDS             complement(902..>1617)
                     /locus_tag="VEA63_12335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD84840.1"
                     /translation="RVEFSSAIDAAGVRADASRFGKLVGLASCAVGLPEKTRRPNLVP
                     TEWVATRAFQRRRPLWLMGAAAVAVGLLPLIRHADRAAENAARRTAEIETRLQPLRVT
                     AAQRAAELAEIARTREQVEALRSVVAARASWSEFLADLQSRLADVGDAWLEELSIVET
                     KRDLAEDAASPALRLRVSGCLLDGIDGANAFDRAKRLLASVAESPFIAAIEDERFDGA
                     RAGVLRFDITLLANAEKRL"
BASE COUNT          294 a          632 c          424 g          267 t
ORIGIN      
        1 cacgccgacc gttcgctttg cccgcgtgaa tctgccgtag tctcctccgc aaacgccagc
       61 ggacgctcag cggcaaccca ctcgaccgtc accgtgaaac gcgagtcgcc gcccgcaacc
      121 aacagccttg gctcttcact atgcccattc gccagcgacc tgatctccgc gccgtcgatt
      181 cgcgctgcct ccacggaccg caccgtgatc ggctgatcag agaccgtgaa tccatcgagc
      241 aatgctcgca cgctcgccgt gccggcaatg aaggtcagct tgaacgcccg accagcgagc
      301 aaacccggca cgcgaacaga gcgcgtcgaa gcgaactcga aggtgtcgtc agctccacgc
      361 tggcgttcgg cgccctctgt ttccacgcgc tcccgttgca cactcacgag tcgcgacggc
      421 cgcgacgcga acaacgtgcg caataaatac tccacgcgta accgatcgag aaacacggcg
      481 tcaatgagcg ccggcgccgg acccttgccg gcatactccg caaacccgaa atgctcgtct
      541 ggcgccaatt cgacgccgtg ctgcgctgct gccgtgcgca tccgcgcggc aaacgtcgcg
      601 agatcgaaat acgcctccgc cctctgccgc ggcacctcgc ctcgttcaag acgatctgca
      661 acccgcaggt tcaacaccgc gagttccgct cgcgcgtctg cgagatcggc catcgcgtcg
      721 aaatcggccg cggtcgcggc agcgagcgtc ttttcttcgt ccctgagccg cgcaagtttc
      781 tcctctgccc gccggcccgc ctcgacgcgc tcgcgccacc accacccttc gcccaccgcg
      841 atcaggacga tcgcggccaa cacggcgaag aatcgcggat gttctcgcaa ccgccggacg
      901 ctcatagtcg tttctccgcg ttcgcgagca gcgtaatatc gaaacgcaac acgcccgcgc
      961 gagcgccgtc gaaacgctcg tcctcaatcg ccgcgataaa cggcgactcc gccacgctcg
     1021 cgagcaaccg cttcgcccgg tcgaacgcgt tcgcaccgtc aatcccgtcc aacagacaac
     1081 cgctcacgcg caaccgcagc gccggcgatg cggcgtcttc cgccaaatct cgtttcgtct
     1141 ccacaattga cagctcctcc agccacgcat ctccgacgtc tgcgagccgg ctttgaagat
     1201 ccgcgagaaa ctcgctccaa ctcgctcgcg cagcgacgac gctacgcagc gcttcgacct
     1261 gttctcgcgt cctcgcaatt tccgcgagct ccgctgcgcg ctgtgcagcc gtcactcgca
     1321 gcggctgtaa tcgcgtttcg atttccgccg tccgcctcgc cgcattctcc gcggcgcgat
     1381 ccgcgtgacg gatcagcggc agcaatccca ccgcaaccgc cgccgccccc atcagccata
     1441 acgggcggcg gcgctgaaac gctcgcgttg ccacccactc ggtgggcacc aggtttggcc
     1501 ttcgtgtttt ttccggaagt ccgaccgcgc aactcgccaa ccccaccaac ttcccgaatc
     1561 gcgatgcgtc tgcccgcacg cccgccgcat cgatcgccga agaaaattcc actcgcc
//
LOCUS       DASPLT010001203         1238 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_218695, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001203 DASPLT010000000
VERSION     DASPLT010001203.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1238)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1238)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1238
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_218695"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..212)
                     /locus_tag="VEA63_12340"
     CDS             complement(<1..212)
                     /locus_tag="VEA63_12340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HYD84841.1"
                     /translation="MNRRDFLVRSSLLVSAGLLPRVSLFSAEPPAKASPMPPVTEFRP
                     LRRGVGLFTGRGGSIGWLSNKDALAIV"
     gene            211..>1238
                     /locus_tag="VEA63_12345"
     CDS             211..>1238
                     /locus_tag="VEA63_12345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD84842.1"
                     /translation="MISIDTMKHRRRNAALVYDLLRRPRVHSHVVSSVENHSKQRPSE
                     SRALMSPILVPPAADGSPQKSQLLLREQLQRDARAISRIAYTLSAIMNARPRLLLLLI
                     AAFVSLASIATAADRKFDMLAVAEQFPLQPSRQVDAPAHPSSYALIGGGFIEAGDPTA
                     GEHPPAASTVTAALQDVLTSAGYTLAPSGVTPTAALIYHWGLIRDERMPLRVPFRLEP
                     NLRTRLSLVTPAARFPSIEERIISRRDYTPAALQTIEYPDVRDALDLARDGVYFVVVS
                     AYDYAALARGETNLLWRTKLTAPLNSGALADVVHSLLRNAAPYLGRHLARRDSLKAPL
                     SASPVASDT"
BASE COUNT          215 a          505 c          301 g          217 t
ORIGIN      
        1 acgatcgcca gcgcatcctt gttcgatagc catccgatcg atccgccgcg ccccgtgaac
       61 aaaccgaccc cgcgccgcag cggacgaaac tccgtcaccg gcggcatcgg cgacgccttc
      121 gccggcggct ctgcggaaaa caacgacacg cgcggcaaca gccccgcgga caccagcaac
      181 gacgaacgca ccagaaaatc gcgacggttc atgatctcca tcgatacgat gaaacaccgt
      241 cgccgcaacg ccgcactcgt ttatgacctg ctgcgccgcc cccgcgtcca ttcgcacgta
      301 gtttcttcgg ttgaaaacca ctccaagcag cgccccagtg agtcgcgcgc gctgatgtcc
      361 ccaatcctcg tgccgcccgc cgctgacggt tctccgcaaa aatcgcaact cctgctccgt
      421 gaacaactgc aacgcgacgc gcgtgcaatc tcacgcatcg catatacctt gtccgccatc
      481 atgaacgctc gcccccgcct ccttctcctc ctcatcgccg ccttcgtgtc actggcttcc
      541 atcgccaccg ccgcggatcg aaaattcgac atgctcgccg tcgccgagca gttcccgctc
      601 caaccaagcc gtcaagtcga cgcgcccgcg cacccttcgt cctacgcgct catcggcggt
      661 ggcttcatcg aagccggcga ccccaccgcc ggcgaacatc cgcccgccgc ttcgaccgtc
      721 accgccgcgc ttcaagatgt tctcacgtcc gccggctaca cgctcgcacc ttcgggagtc
      781 actccgaccg ccgcactgat ttatcattgg ggcctgattc gcgacgaacg catgccgttg
      841 cgcgtcccgt tccggctcga gcccaatctc cgcacccgcc tctcacttgt cacgcccgcc
      901 gcgcgcttcc cctccatcga agagcgcatc atctcgcggc gcgattacac gccggcagcg
      961 ctgcagacga tcgagtatcc agatgttcgt gacgccctcg atctcgcccg cgatggcgtt
     1021 tatttcgtcg tcgtctccgc ctacgactac gccgcgctcg ctcgcggaga aaccaatctt
     1081 ctctggcgaa ccaagctcac tgccccgctc aacagcggcg ctctcgccga cgtggttcac
     1141 tcgctcctcc gcaacgccgc gccctatctc ggccgccacc tcgcccgccg cgattcgctc
     1201 aaggcgcctc tctccgcgtc gccggtcgca agcgacac
//
LOCUS       DASPLT010001204         1397 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_115842, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001204 DASPLT010000000
VERSION     DASPLT010001204.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1397)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1397)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1397
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_115842"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            234..1214
                     /locus_tag="VEA63_12350"
     CDS             234..1214
                     /locus_tag="VEA63_12350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0016653 - oxidoreductase activity, acting
                     on NAD(P)H, heme protein as acceptor [Evidence IEA];
                     GO_process: GO:0006784 - heme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="COX15/CtaA family protein"
                     /protein_id="HYD84843.1"
                     /translation="MASTSHPLRNASHKPALAWFAGFGSVWVFLLVVLGAFTTSIGAG
                     MVFPDWPLSNGSLNPEGWLTNVAMFAEHSHRLTAAVMSLITIVLAFWLHRTEQRAWLR
                     KLAWFAVALVLVQALVGGLRVLLDHLHVEMVDTSVGRLFAMLHACLAQLFVCTLIAVA
                     AACSRNWIAGGAPVSCAVRRAGAICCVLFFIQLAIAAVMRHSFAGLAIPTFPYSTPDD
                     GLLPELWNFRIGIHFAHRLMAVVLAIAVIWFSFKLRIDRGASVPMRIGASLIVSLIAI
                     QIVLGAQIIWTQRSPGTTTAHVLIGALTLALTFWLTWLAHRDVVEGKARA"
     gene            1211..>1397
                     /locus_tag="VEA63_12355"
     CDS             1211..>1397
                     /locus_tag="VEA63_12355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009508190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX farnesyltransferase"
                     /protein_id="HYD84844.1"
                     /translation="MSGVSAAAPRARFSDYLELTKPRLSLLSVLTALVGYLAAKPPFD
                     GMKLFLLVLGTSLAAAGV"
BASE COUNT          199 a          498 c          401 g          299 t
ORIGIN      
        1 gtcgtccgct ttctccgccc gcttgcaacc acccgccgcg agcagcaatc ccggcaccgc
       61 aacgccgacc atcaaccagc gacgcgtcgc acgccgaaac gaaccggcgg atttcgtcac
      121 acgacggccg tgcgcattag ttttcatcac actacggatt cgccgcggga aaaatttgtc
      181 aaagggtttg cggccatcag caacgaccct tctgctggca aactttcccg ctcatggcct
      241 ccacttccca ccctctccgc aacgcctctc acaaacccgc gctcgcgtgg tttgccggct
      301 tcggcagcgt ttgggttttc ctcctcgtcg tcctcggcgc gttcaccacc agcatcggag
      361 ccggcatggt gtttcccgac tggcccctct cgaacggctc gctgaacccc gagggctggc
      421 tcacgaacgt cgccatgttc gccgaacact cgcaccgcct gaccgccgcg gtgatgagcc
      481 tgatcacgat cgtcctcgcc ttctggcttc atcgcacgga gcagcgcgcg tggctccgaa
      541 agctcgcgtg gttcgccgtc gcgctcgtgc tcgtgcaagc gctcgtcggc ggtctgcgcg
      601 tgctgctcga tcacctgcat gttgaaatgg tggatacaag cgtcggccgg cttttcgcga
      661 tgctgcacgc gtgcctcgcg caactcttcg tctgcacgct catagcggtc gcggcagcct
      721 gctcgcgcaa ctggatcgca ggcggcgcac cggtcagttg tgccgtgcga cgcgccggcg
      781 cgatctgctg cgtcttattc ttcatccaac tcgcgatcgc cgccgtgatg cggcacagct
      841 tcgcggggct cgcgattccc acatttccct attcgacgcc ggacgacgga ctgttgcccg
      901 agctgtggaa tttccgcatc ggcattcatt tcgcgcatcg cttgatggcc gtcgttctcg
      961 cgatcgccgt gatctggttc tccttcaagc ttcgcatcga tcgcggtgcc tccgtcccga
     1021 tgcgcatcgg cgcctcgctc atcgtcagct tgatcgccat ccagatcgtg ctcggcgcgc
     1081 aaatcatttg gacgcaacgt tcgcccggca cgacaacggc gcacgtgctc atcggtgcgc
     1141 tgacgctcgc gctcacgttc tggctgacgt ggctcgcgca tcgcgacgtc gtcgaaggaa
     1201 aggctcgcgc atgagcggtg tttccgccgc ggcgccgcgt gcgcgcttct ccgactacct
     1261 cgagcttacg aagccgcggt tgagtttgct gtcggtgttg acggcgctcg tcggttacct
     1321 cgccgcgaag ccgccgttcg acggcatgaa gctgtttctt ctcgtgctcg gcacctcgct
     1381 cgccgccgcc ggtgtcg
//
LOCUS       DASPLT010001205         1786 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_138141, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001205 DASPLT010000000
VERSION     DASPLT010001205.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1786)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1786)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1786
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_138141"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1641
                     /locus_tag="VEA63_12360"
     CDS             <1..1641
                     /locus_tag="VEA63_12360"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HYD84845.1"
                     /translation="DAELVAACLAGDRAAFGQIVERYQRLLCSLAYSATGQLSHSEDL
                     AQDAFVDAWRELPHLREPEKLRSWLCGILRFKVSRLRRSDGREPVRQADSLDVAEHVA
                     SGDEPAANLAIQKEEQAIMWSALERVPELYREPLILYYREHRSVEHVAAALDLTEDNV
                     KQRLARGRKILQEQVLAFVEGALTRSTPGRVFTLAVLAALPEIATPAKAIGVGAAAVH
                     GGMLAKTTSIATLLASISGVVSAVLTLRANLDQSRTPRERRMVVKITIAAFFSVIGLF
                     VALHLLRGAAFRWWETRAVFAALAQVLVLTFLVFWPFGLIAVMRHMRALRTSERRVHP
                     ELFRDVRDQLGSSAGEYKSRWTMFGVPLVRIRFASPGAGEPPVRAWFAGGDRAYGLIF
                     AWGGLAVAPISVGAISIGFLAVGSASVGVVSFGTMAAGALALGAVTIGVKAYAWLSAL
                     GWETAQSNGFAIARIAAEGPIALARHANDPIARQILANPDAERNQMMFFIVISLLSLV
                     PVACYTRAVRQRLGSREKKSPSSLAHSVDTATRAADER"
BASE COUNT          283 a          605 c          569 g          329 t
ORIGIN      
        1 gacgccgaac tcgttgccgc ctgcctggcc ggcgatcgcg ccgccttcgg tcaaatcgtg
       61 gaacgctatc agcgactcct ctgttcgctc gcctactcgg cgactggtca actcagccat
      121 agcgaagacc tcgcccagga cgcgttcgtc gacgcctggc gcgaactgcc gcacctgcgc
      181 gaaccagaaa aactgcgctc ctggctgtgc ggaattctgc gtttcaaagt cagccgcttg
      241 cgccgcagcg atggacgcga gcccgtgcgc caggccgact cgctcgatgt cgccgaacac
      301 gtcgcgtcgg gcgacgagcc cgcggcgaac ctcgcgattc aaaaagagga gcaggcgatc
      361 atgtggagtg cgctggaacg cgtccccgag ttgtatcgcg agccgctcat cctctattac
      421 cgcgaacatc gttccgtcga acacgtcgcc gccgcactcg acttgaccga ggacaacgtg
      481 aaacagcggc tcgcgcgcgg tcgcaagatc ctgcaggagc aagtgctcgc cttcgtcgaa
      541 ggcgcgctca cccgcagcac ccccggccgc gtcttcaccc tcgcggtgct cgccgcgctg
      601 ccggagatcg ccacgcccgc caaagccatc ggcgttgggg ccgcggctgt gcacggcggc
      661 atgttggcaa aaaccacgag catcgcgacg ctgctcgctt ccatcagcgg cgtcgttagc
      721 gcggtgttga cgctgcgcgc caacctcgat cagtcgcgca cgccgcgcga acgccggatg
      781 gtcgtgaaga tcacgattgc cgcgttcttc agcgtgatcg ggcttttcgt cgcgttgcac
      841 cttttgcgcg gcgccgcgtt tcgctggtgg gaaacgcgtg cggtgttcgc cgctctcgca
      901 caggtgctcg tgctgacgtt tctcgtcttc tggccgtttg gcttgatcgc cgtgatgcgg
      961 cacatgcgcg ctctccgcac ctccgagcgc cgtgtccatc cggagctgtt tcgcgatgtg
     1021 cgcgaccaac tcggatcgtc ggcgggtgaa tacaaaagcc ggtggacgat gtttggcgtg
     1081 ccgctcgttc gcattcggtt cgcatcgccc ggcgccggcg agccgccggt gcgcgcgtgg
     1141 tttgccggtg gggatcgcgc ctatggcttg atcttcgcct ggggcggcct cgcagtcgcg
     1201 ccgatcagcg tcggcgcgat ctcgatcggc ttcctggccg tcggcagcgc gagcgtcggc
     1261 gtcgtctcgt tcggcacgat ggccgcggga gcgcttgccc tcggcgccgt gacgatcggc
     1321 gtgaaagcgt atgcgtggtt gtcggcgtta ggctgggaaa ccgcgcagag caacggcttc
     1381 gccatcgcgc gaatcgccgc ggaaggaccg atcgcgctgg cgcgccacgc gaacgatccg
     1441 atcgcacgcc agatcctggc caacccagat gcggagcgaa accaaatgat gtttttcatc
     1501 gtgatctccc tcctctcgct ggtgcccgtc gcctgttata cccgagccgt gcgacaacgt
     1561 ctcggcagtc gggagaaaaa atcgccgagc agtctcgctc attccgtgga caccgcgact
     1621 cgcgcggctg acgagcggta gccggcgctt cagcgcgccc cacgggaaac gcaaagcggt
     1681 cggcaaaagc gcgagcgtca cttgtatcca tcgggaaacc acgagcggtt tacctcggaa
     1741 tcctcccggc gtagcaccct gtccgatctg ttacgctttc cacggg
//
LOCUS       DASPLT010001206         1255 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_280269, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001206 DASPLT010000000
VERSION     DASPLT010001206.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1255)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1255)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1255
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_280269"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..701)
                     /locus_tag="VEA63_12365"
     CDS             complement(<1..701)
                     /locus_tag="VEA63_12365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HYD84846.1"
                     /translation="MKFRSLFTFTLARVFPLTVAALVATLAASAQEKPEEASPERARA
                     NFARPIVLAPDDVRAFPDAPAGFNTRRNDVPAGRIESFNYDSAVTGTRRKANVYLPPG
                     YTAEKKYPVLYLLHGIGGDETEWIRFAQPDALFDNLIADGKAVPMIVVIPNGRAVAND
                     QTPPSDRIFAPENVAGFAAFERDLLDHLIPAIEAKYSTLTDREHRALAGLSMGGGQTF
                     NFGLGNLDTFAWIGA"
     gene            complement(795..>1255)
                     /locus_tag="VEA63_12370"
     CDS             complement(795..>1255)
                     /locus_tag="VEA63_12370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HYD84847.1"
                     /translation="TGVMDRVPERIKHVVYLDAAVPDDGQSIWDLFGNRGPVDNDRFK
                     DGFMQVPWVKPDAKPPHNVKQSIKCFNQPVSYKNPAAKALNVTYVAFVPKGKSAEERA
                     KTDKSWQRAESRGWTIRTFPGGHVAQQEDPRGVATLIEDSINDKNKAVAQ"
BASE COUNT          220 a          352 c          448 g          235 t
ORIGIN      
        1 aacgcgccga tccaggcgaa ggtatccaga ttcccgagac cgaagttgaa cgtctgcccg
       61 ccacccatcg agagtccggc gagcgcgcga tgctcgcgat ccgtcagcgt ggaatacttc
      121 gcctcgatcg cgggaataag gtgatcgagc agatcgcgct cgaacgccgc gaagccggct
      181 acgttctcgg gtgcgaaaat gcgatctgac ggaggtgtct ggtcgttggc caccgcacga
      241 ccgttgggga tcacgacgat catcggcacc gccttgccgt cggcgatcag gttgtcgaaa
      301 agcgcgtcag gctgggcgaa gcggatccac tcggtttcgt cgccgccgat gccgtgcagg
      361 agatacagaa cgggatactt cttttcggcg gtgtaaccgg gcggcaaata cacgttggcc
      421 ttgcggcgcg tgccggtgac ggcggagtcg tagttgaacg attcgatgcg gccggcgggc
      481 acgtcattac ggcgtgtgtt gaagccggcg ggagcgtcgg gaaacgcgcg cacgtcgtcg
      541 ggcgccagca cgatggggcg cgcgaaattg gcgcgagcgc gctcgggtga ggcttcctcg
      601 ggtttctcct gcgctgacgc ggccagcgtg gcgacgaggg ctgcgacagt gagagggaaa
      661 acgcgagcga gcgtgaaagt gaaaagagag cggaatttca tgaggtgcgg gcgattgaga
      721 aatgtagggg cggaccttgt gtccgcccgc tgagggtgtg gacgagccac gcccctacac
      781 ggagcgcatt ggctctactg ggcgacggcc ttgttcttgt cgttgattga atcctcgatg
      841 agcgtcgcga cgccgcgcgg gtcttcctgc tgggcgacgt ggccgccggg aaaggtgcgg
      901 atcgtccagc cccgcgactc ggcgcgctgc cagctcttgt cggttttggc gcgctcctcg
      961 gcggacttgc ccttcggcac gaacgcgacg taggtgacgt tgagcgcctt ggcggcgggg
     1021 tttttgtagg agacaggctg gttgaagcac ttgatcgact gcttcacgtt gtgcggcggc
     1081 ttggcgtcgg gtttgaccca tggcacctgc atgaaaccgt ccttgaagcg gtcgttgtcg
     1141 acgggaccac ggttgccgaa aagatcccag atcgactgcc cgtcatcggg gacggcggcg
     1201 tcgagataca cgacgtgttt gatgcgctcc ggaacgcgat ccatcacacc ggtga
//
LOCUS       DASPLT010001207         1857 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_157582, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001207 DASPLT010000000
VERSION     DASPLT010001207.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1857)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1857)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1857
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_157582"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..864
                     /locus_tag="VEA63_12375"
     CDS             <1..864
                     /locus_tag="VEA63_12375"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HYD84848.1"
                     /translation="ERTEQNISHTHMPYIDSHTHFWDRSVMPYLWLHEVPSIWHRHAP
                     ETLHAEAAGDLPEKIVFVECGAPALDEVRWIEQLAAHEPRLAAIVARIVINTGAQTTA
                     AIAELKTHPLVRGVRHHFEHDALDYCARPEFIAGVRELPAAGLSFDICCKHPQLPAVI
                     ELVRQCPDTGFILDHAGKPGIRDGLLDPWREHIRTLATFPNVVCKLSGLVTEADHRHW
                     TPTQLQPYVAHLLETFGPSRLLFGGDWPVAKLASSYHRWLDTARALAAHLPPAEQNAI
                     FHHNAARTYRL"
     gene            931..>1857
                     /locus_tag="VEA63_12380"
     CDS             931..>1857
                     /locus_tag="VEA63_12380"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HYD84849.1"
                     /translation="MTSFPLRPFGQTGERLPAIAMGCGFPGFAGFDRSIETIRRAADL
                     GIRYFDTSPLYCSGASQAIVGAAFAVPTPPHFLATKVGHLKVAGHFRSVEALHVQLRE
                     NLRLLRRDSVDLLQLHEADWDNWWSDRADVAPCELFDVEDSFDFAHAPAIQFLREAKA
                     QGLCRHIGITGNNARHLTRLIHEIDGLDSVLIAYNYTPLNITVLDDLLPAARTKGLAV
                     IVAGLFTFVNSIPPGWRTEGTYFGPHADRQLAELKKLQQTCGLSMAELAVRFVATDER
                     LATTLVGACHPHEVEQNVASFHRGKLPADLHAA"
BASE COUNT          337 a          720 c          449 g          351 t
ORIGIN      
        1 gaacgaactg agcagaatat ttcccacacc cacatgccct acatcgattc gcacacgcat
       61 ttctgggacc gctccgtcat gccttatctt tggctccacg aagtcccctc gatctggcat
      121 cgccacgccc ctgagacgct gcacgccgaa gctgccggcg atctcccgga gaaaatcgtc
      181 ttcgtcgaat gcggcgcgcc cgcactcgac gaagttcgat ggatcgaaca actcgccgca
      241 cacgaaccgc gtctcgccgc gatcgtcgca cgcatcgtca tcaacaccgg tgcccaaacc
      301 accgccgcca tcgccgagtt aaaaacgcat ccgctcgtcc gcggcgtgcg ccatcatttc
      361 gaacacgacg ccctcgacta ctgcgcgcga cccgagttca tcgccggcgt tcgcgagttg
      421 cccgccgccg gtctgagttt cgacatttgc tgcaaacacc cgcagctccc cgccgtgatc
      481 gaactcgtcc gccaatgccc cgacaccggc ttcattctag atcacgccgg caaacccggc
      541 attcgcgacg gtttgctcga tccttggcgc gagcacatcc ggacgctcgc cacattcccc
      601 aacgtcgtct gcaaactctc cggcctcgtc accgaagccg atcatcgcca ttggacgccc
      661 acccagctcc agccctacgt cgcgcacctg ctcgagacct tcggcccctc acgcctcctc
      721 ttcggcggcg attggcccgt cgccaaactt gcgagcagct atcaccgctg gctcgacacc
      781 gctcgcgcac tcgctgcgca ccttccgccc gccgaacaaa acgccatctt ccaccacaac
      841 gccgcacgca cgtatcgcct gtgacgacaa catcgccgcc ccggcggccg tgttgcggtt
      901 agtgagcatt cgcgtgattc gcgggcaacc atgacatcat tccccctccg tcccttcggc
      961 caaactggcg agcgcctccc cgcgatcgcg atgggctgcg gctttcccgg attcgccggc
     1021 ttcgaccgct ccatcgagac tatccgacgc gccgccgatc ttggcatccg ttatttcgat
     1081 acgtctccgc tctattgctc gggcgcatcg caagcgatcg tcggcgccgc ctttgcggtg
     1141 ccaactccac cgcattttct cgcgaccaaa gtcggccacc tgaaggtggc cggccacttt
     1201 cgctccgtcg aagccttgca cgttcaactc cgcgaaaatc tccgcctgct tcgccgcgac
     1261 tccgtcgacc tgctccaact ccacgaagcc gattgggaca attggtggag cgatcgcgcc
     1321 gacgtcgcgc cgtgcgagct gttcgacgtc gaagacagtt tcgatttcgc ccacgcaccc
     1381 gccattcagt tccttcgcga agccaaagca cagggtcttt gtcgccacat cggcatcacc
     1441 ggcaacaacg cccgtcatct cactcgtctc attcacgaaa tcgacggcct cgattccgtt
     1501 ctcatcgcct acaactacac gccgcttaac atcactgtgc tcgacgacct tcttcccgcc
     1561 gcgcgaacga aaggcctggc cgtgatcgtc gccggcttgt tcaccttcgt gaattcaatt
     1621 ccccccggct ggcgaaccga aggcacctat ttcgggccgc acgccgatcg ccagctcgcc
     1681 gagctgaaaa aactccagca aacctgcggc ctctcgatgg ccgaactcgc cgtgcgcttc
     1741 gtcgccaccg acgaacggct cgccaccacg ctcgtcggcg cgtgccatcc gcacgaggtc
     1801 gagcaaaacg tcgcctcctt ccatcgcggc aaacttcccg ccgatcttca cgctgcc
//
LOCUS       DASPLT010001208         1256 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_138218, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001208 DASPLT010000000
VERSION     DASPLT010001208.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1256)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1256)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1256
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_138218"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..884
                     /locus_tag="VEA63_12385"
     CDS             <1..884
                     /locus_tag="VEA63_12385"
                     /inference="COORDINATES: protein motif:HMM:NF023167.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YdbH domain-containing protein"
                     /protein_id="HYD84850.1"
                     /translation="VEMSGGGVIDAGASRGVMRIEWKDGGVRHEADGWTLEGVSFAGE
                     FAIDTEKGTIESTLPLSVVVQTMTTARFGARGLAVIARLNADRTLAVEEARIEIAGGE
                     VTADPTTVALTPMASELNLRITNVGLQDVAALVPSGLLESRGRINGAVRVGWSEAGGF
                     KLGEGNLVLEQTEPAMVRLAPTPGFLTAQITERVPLLPASFGPLARWTSLRNPAFEEI
                     RDIELGRTGLQIESLSVQLSPQGDRDGRTARVRMTGRPARPDSVVGAVTFEVNVAGPL
                     SEVLRLGMNQSFSVETR"
     gene            930..1112
                     /locus_tag="VEA63_12390"
     CDS             930..1112
                     /locus_tag="VEA63_12390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD84851.1"
                     /translation="MRHRLPVVACIALLAGCVHVKLDPVQVHAVVDVNVKVDRALDDF
                     FGDLDQKSATIENPAR"
BASE COUNT          213 a          309 c          500 g          234 t
ORIGIN      
        1 cggtggagat gagcggtggt ggagtgatcg atgcgggagc gtcgcgcggc gtgatgcgga
       61 tcgagtggaa ggacggcggc gttcgtcacg aggcggacgg atggacgttg gaaggcgtgt
      121 cgtttgccgg cgagtttgcg atcgatacgg agaaggggac gatcgagagc acgctgccgt
      181 tgagcgtggt cgtgcaaacc atgacgacgg cgcgtttcgg cgcgcgggga ttggcggtga
      241 tcgcgaggtt gaacgccgat cgcacactgg ccgtggaaga ggcgaggatc gaaatcgcgg
      301 ggggcgaagt gacggcggat ccgacgacgg tggcgttgac gccgatggcg tcggagttga
      361 acttgcggat caccaatgtg gggttgcagg acgtggcggc actcgtgccg tcgggacttt
      421 tggagtcgcg cgggcggatc aatggagccg tgcgggtcgg gtggagcgag gccggcggat
      481 ttaagctcgg cgaaggaaat ctggtgctcg agcagacgga gccggcgatg gtgcggctcg
      541 cgccgacgcc ggggttttta accgcgcaaa tcacggagcg agtgccgttg ctgccggcgt
      601 cgttcggacc gttggcgcgc tggacgagtt tgcggaatcc ggcgttcgag gagattcgag
      661 atatcgagtt gggacggacg gggctgcaga tcgagagcct gagcgtgcag ctgtcgccgc
      721 agggcgatcg cgatggccgg acggcgcgcg tccgaatgac cgggcggccg gcgaggcccg
      781 acagtgtggt cggtgcggtg acgttcgagg tgaacgtggc ggggccgttg tcggaggtgc
      841 tgcggctggg gatgaaccaa agcttttcag tggagacgcg ttgaggaaac ttctgttgcc
      901 agaaagcgtc ttggggggaa catatgagca tgcgccatcg tttaccggtc gttgcttgca
      961 tcgcgctcct cgcgggctgc gttcacgtga aactcgatcc ggtgcaggtg cacgcggtgg
     1021 tcgacgtgaa tgtgaaagtc gaccgcgcgc tcgacgattt cttcggcgac ctcgaccaga
     1081 agtccgccac gatcgaaaac cccgcgcgtt gagcgcgcct tgattcagca cacgatgaaa
     1141 aacgtttcca tgtttcgcat gctggcggtg gcgtttgccc tgctggtggg tttcagcgcg
     1201 gtcgctttgg cggccgagga tctcggcgcg gtgaaggcgc gtatgacgca gcggat
//
LOCUS       DASPLT010001209         1278 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_199502, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001209 DASPLT010000000
VERSION     DASPLT010001209.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1278)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1278)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1278
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_199502"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..963)
                     /locus_tag="VEA63_12395"
     CDS             complement(<1..963)
                     /locus_tag="VEA63_12395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HYD84852.1"
                     /translation="MNRRSFISSVAVASAATAVSPSRLFSASTPSRPKLGLIGCGWFG
                     GVNLGNFLRHTEVDVVSLCDPNTHALAKTLALVAEKQTTAPKTYADFREMLAATPHDI
                     VLVATPDHWHALPAIAAIQAGADVYLEKPIGVNVIEGEALVAAARKYNRVVQVNTQRR
                     SNPVYHDLREKFIRSGRLGEIGLVETYSYLPARPAGDIPPAEPPAHLNYDLWTGPAPS
                     IPYRTAFEVRGWRSFMEFGNGQIGDLGVHSIDLARWLLGLGWPESIHSTGGIYVDRAS
                     ASNISDTQRAVFRYPHLDLSWEHRTWGVSPIPLRHWSDQWGTRLI"
BASE COUNT          241 a          274 c          525 g          238 t
ORIGIN      
        1 gatgaggcga gtgccccact ggtcggacca gtgacgtagt gggattggcg atacacccca
       61 ggtgcgatgt tcccagctga ggtcgagatg aggataacgg aagacggcgc gctgcgtgtc
      121 ggagatgttg gaagcgctgg cgcgatcgac gtagatgccg ccggtggagt ggatggactc
      181 gggccagccg aggccgagga gccagcgggc aagatcgatg gagtgcacgc cgaggtcgcc
      241 gatctggccg ttgccgaact ccatgaacga gcgccagccg cgcacttcga aagcggtgcg
      301 gtaaggaatg gagggagcgg ggccggtcca gagatcgtag ttgagatgcg cgggtggttc
      361 ggcgggagga atgtcgccgg cggggcgcgc gggaaggtag gagtaagttt cgacgaggcc
      421 gatttcgccg agccggccgc tgcggatgaa tttttcgcgc aggtcgtgat acaccggatt
      481 gctgcggcgc tgggtgttca cctggacgac gcggttgtat tttcgtgcgg cggcaacgag
      541 cgcttcgcct tcgatcacgt tgaccccgat cggcttttcg agatagacgt cggcgccggc
      601 ctggatggcg gcgatggcgg gaagcgcgtg ccagtgatcg ggggtggcga cgagcacgat
      661 gtcgtgcggc gtggcggcga gcatttcgcg gaagtcggcg taggtcttcg gcgcggtggt
      721 ttgtttttcc gcgacgaggg cgagggtttt ggcgagtgcg tgggtgttgg gatcgcagag
      781 cgaaacgaca tcgacttcgg tgtggcggag aaaattgccg aggttgacgc cgccgaacca
      841 gccgcagccg atgaggccga gtttgggtcg ggaaggggtg gaggcggaga aaaggcggga
      901 ggggctgaca gcggttgccg cgctggcaac ggcgacggag gagatgaagg agcggcggtt
      961 catggggtat tcggagagaa cgggcggctg atgggggcgg acgatggtcg acggagttga
     1021 cgcgaaggga aagggattga tgcggaggcg agagcgcaga cggagttttt ttaaccgcta
     1081 atctgcgctg atttttcgga gggagagggg cggatgaagc ggtgcgtgct tcgcctgatc
     1141 ggcgaagcgc gggcggggtt ggggaaaagg ggatcgtgaa aactgagaat tggggatttg
     1201 gaattttgaa attgttagga gcgggacggg taaattttag atgcgcgatt cccggtgtcc
     1261 gatgtatgtt ttcaagat
//
LOCUS       DASPLT010001210         2441 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_199554, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001210 DASPLT010000000
VERSION     DASPLT010001210.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 2441)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2441)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2441
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_199554"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(253..1638)
                     /locus_tag="VEA63_12400"
     CDS             complement(253..1638)
                     /locus_tag="VEA63_12400"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007363140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006206 - pyrimidine nucleobase metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HYD84853.1"
                     /translation="MTKRTIPARRFIKPSFEFLIEKKRDGGEFTQEEIRYIIDSTLDG
                     EMPKHQLAALMMAIYFSNMSAQETADLTEEMMLSGEVIDLSSIAKPKIDKYSTGGVGD
                     KTSLVLVPLAMASGVVVPMMCGVEHDYVINTLDKLSSIPGFKGQLSIDQFISQLGRIG
                     GAVVQQSDALAPADAKFHELRKETGTIPSLPLITGSVLSKKLAEGSEGLVIDVKWGNG
                     SFIKDLEQAKQLARSMTRVGRSMKRRCVALVTDMNQPLGDTVGTSLEVKEAIQLLKGE
                     GPEDMKELVLKLGMEIVRLAGVAGSTLSAKQTVQRHLTDGSALQKFKDLVKAQGGDIS
                     FIDHPEKFPAARHIRKLPAPKRGYVHTINAAMIARGVHLLGAGIEPSASHGNHNHGSH
                     GNHHAKVDHAVGVSEIKKVGTQVKQGEPLMMIHYNDEAKLEQALEYFKNAYRLAPKRP
                     TPPPLIVERVA"
     gene            complement(1829..>2441)
                     /locus_tag="VEA63_12405"
     CDS             complement(1829..>2441)
                     /locus_tag="VEA63_12405"
                     /inference="COORDINATES: protein motif:HMM:NF017945.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="urate hydroxylase PuuD"
                     /protein_id="HYD84854.1"
                     /translation="VIIPGQRRMVAAMREGRRPDPMEGRRGKQRSVHNNYFTLPVVFT
                     MVSNHYASTYGHAYNWAVLALIAGAGVAIRHFFNRRHKGVLAWRYLGIGAVLLGATTW
                     WTMPRVPELPEVEGEVTFDRVRAIVGERCLACHTPAPTFPGILQPGAGVLLHTPDGLT
                     RNAERIYQQVVATRIMPLGNATAMTDQERAVIASWVRNGAHER"
BASE COUNT          456 a          809 c          691 g          485 t
ORIGIN      
        1 ggcgcagtgg tgagtccggt gcattggcag gaaaaatgga gttttcgagc aagccaacgc
       61 ccactcaccg gagcaagcac aactctcagg atcggactcg cggcgtcgcg tgccagatct
      121 cgatctcggt caaacaggac cgtcctttcg gaacgacctc cgcagtcgcg ctgcgaaacc
      181 cgtgcgaacg ttcccaaaca aaaggccgac cggaaaccgg tcggcctcga gaaagatcgc
      241 gacagatggc gcttacgcca cgcgctcgac gatcaacggc ggcggcgtcg gccgcttcgg
      301 cgcgagccgg taggcgttct tgaaatactc cagcgcctgc tcgagcttcg cttcgtcgtt
      361 gtagtgaatc atcatcaacg gctcgccctg cttcacctgc gtgccgactt tcttgatctc
      421 cgaaacgccg accgcgtgat cgaccttcgc gtgatgattg ccgtggctgc cgtggttgtg
      481 attgccgtga ctcgcgctcg gctcgatgcc agcgccaagc aggtgcacgc cgcgcgcgat
      541 catcgccgca ttgatcgtgt gcacatagcc gcgtttcggc gccggcagct tccggatgtg
      601 acgcgccgcc ggaaattttt ccggatggtc gataaacgaa atgtcgccgc cctgcgcctt
      661 caccagatcc ttgaatttct gaagcgccga tccgtcggtg agatgacgct gcaccgtctg
      721 cttcgctgaa agcgtcgaac ccgccacgcc ggcgagccgc acgatttcca tgccgagctt
      781 caacacgagc tccttcatgt cctcgggacc ttcgcccttc aggagttgaa tcgcttcctt
      841 tacttccaac gacgtcccaa ccgtgtcgcc cagcggctga ttcatgtcgg tgacgagcgc
      901 cacgcaacgg cgcttcatcg agcggccaac gcgcgtcatc gagcgcgcaa gctgcttcgc
      961 ttgctcgaga tccttgatga aggagccgtt gccccatttc acatcgatca cgaggccttc
     1021 cgagccttcg gcgagcttct tcgaaagcac gctgcccgtg atgagcggca aactcggaat
     1081 cgtgccggtc tccttccgca attcgtggaa cttcgcgtcg gccggcgcca gcgcgtcgct
     1141 ctgctgcacg accgcgcccc cgatccggcc aagctgggag atgaattggt cgatcgagag
     1201 ctgccctttg aatcccggaa tgctcgagag cttgtcgagc gtgttgatca cgtagtcgtg
     1261 ctcgacgccg cacatcatcg gcaccaccac gccgctcgcc atcgcgagcg gcacgagcac
     1321 gagcgaggtt ttatcgccca cgccgcctgt ggaatacttg tcgatcttcg gcttggcgat
     1381 cgaggacagg tcgatcacct cgcccgataa catcatctcc tccgtgagat ccgccgtctc
     1441 ttgagcggac atgttcgaga aatagatggc catcatcagc gccgccaact ggtgcttggg
     1501 catttcgcca tcaagcgtgc tgtcaatgat gtagcggatc tcttcctgcg tgaactcgcc
     1561 gccgtcgcgc ttcttctcga tcagaaactc gaagctgggt ttgatgaacc ggcgggccgg
     1621 aatggtgcgt ttcgtcatgt gggtatagaa tgatacccgc tcgcgttgcc gcggtgcgga
     1681 aaagtgtgcg ataaaagccg cttatgacaa tttgtcgagc ctaaaggcct agcctcggaa
     1741 acggcggcgt tcccatctga agtcacgaca caaactatac gcctatagta gtatagcttc
     1801 aacctgcccg cacaatcggg cgcccaccct atcgttcgtg cgcgccgttt cgcacccaac
     1861 tggcgatcac cgcgcgctcc tgatccgtca tcgcggtcgc attgcccaag ggcatgatcc
     1921 gcgtcgccac aacctgctgg taaattcgtt cggcgttccg cgtgaggccg tcaggcgtgt
     1981 gcaataacac gccggccccc ggttgaagga tcccgggaaa cgtcggcgcc ggcgtatggc
     2041 atgcgagaca acgttccccc acgatcgctc gcacgcggtc aaacgtgact tcgccttcga
     2101 cctccgggag ttccggcacg cgcggcatcg tccaccaagt cgtggcaccg agcagcaccg
     2161 cgccgatccc caaatagcgc cacgccagca cgcctttgtg ccgccggttg aaaaagtgcc
     2221 ggatcgccac gcccgctccg gcgatcaacg ccagcaccgc ccagttgtaa gcgtgaccgt
     2281 aagtcgacgc gtagtgattg ctcaccatcg tgaacaccac cggcagcgtg aaatagttgt
     2341 tgtgcacgct ccgctgcttg ccgcgacgtc cctccatcgg gtccggcctg cgtccttcgc
     2401 gcatcgccgc caccatgcgc cgctgccccg gaatgatcac g
//
LOCUS       DASPLT010001211         1226 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_38445, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001211 DASPLT010000000
VERSION     DASPLT010001211.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1226)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1226)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1226
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_38445"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..782
                     /locus_tag="VEA63_12410"
     CDS             <1..782
                     /locus_tag="VEA63_12410"
                     /inference="COORDINATES: protein motif:HMM:NF021616.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD84855.1"
                     /translation="IEIAGPSTALAYCWSQSIDQQGRLIAWGHPTKLEGRVASALLLA
                     NFIGNASAPLIRRSALSETGLYLTRVRQNGGQGCEDWDLHLRLAERFSLCCAPAYLVS
                     YRQGRANMSLDAASMTASYEAMIRRAWERNPDMFSPLRSWSRSRFHTYLMNRCYAWGD
                     YARALQCLGRSLSLDPAAWLNARSYRVGAGALLHLATGGKFRRFRSPPPLASSTRPPP
                     LIPVAHTSLFSRIERQRLEVALRETARPGHRRLRPRFTFSV"
     gene            860..>1226
                     /locus_tag="VEA63_12415"
     CDS             860..>1226
                     /locus_tag="VEA63_12415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD84856.1"
                     /translation="MKINHPLRTFAPIVLGALLLTAFSSLQAQDPDLDVALPSPPSAV
                     PVPAAAGDLLNPLESFIATTRPTLSYSYADVDGLLSAPGVRSETSLQTISATFSAELV
                     ESLLIEYRPMWITYSNDAFD"
BASE COUNT          186 a          472 c          319 g          249 t
ORIGIN      
        1 gcattgaaat cgccgggccg agcaccgctc tcgcctactg ctggtcgcaa agcatcgatc
       61 agcaggggcg actgatcgca tggggccatc ccacgaagct cgagggccgc gtcgcctccg
      121 cgctgctgtt ggccaacttc atcggcaacg ccagtgcccc tctcatccgc cgctccgcgc
      181 tgtccgaaac gggactctac ctcacacgcg ttcgtcaaaa cggcggacaa ggctgcgagg
      241 actgggatct ccacctgcgg ctcgccgagc gcttcagcct gtgctgcgcc cccgcttatc
      301 tcgtcagcta tcggcagggc cgtgccaaca tgagtctcga tgcggcgagc atgacggcgt
      361 cctacgaggc gatgatccgt cgcgcctggg aacggaatcc cgatatgttc agccccctcc
      421 gctcatggtc gcgcagtcgt tttcatacct atctgatgaa ccgctgctac gcttggggcg
      481 actacgcccg cgctctgcag tgtctcgggc gctcgttgtc tttggatccc gcggcgtggc
      541 tgaatgcccg cagttaccgc gtaggtgccg gggctttatt gcacctcgcc accggcggta
      601 aattccgccg cttccgctct ccgcctccgc tcgcctcatc gacccgaccg cctccactga
      661 ttcccgttgc tcacaccagt ctcttttctc gaatcgagcg ccaacggctc gaagtcgccc
      721 ttcgcgaaac cgcccggcct ggccaccgcc gtctccgccc tcggttcacc ttctccgttt
      781 gatgacgacg gcaatcgcat gccgcgccgc tcccgccaca gctcagccag cttcaccggc
      841 tcgcttcgcc ggtcttcgca tgaaaatcaa ccaccctctc cgcacctttg cgccgattgt
      901 cctcggcgcc ctgctgctca ccgccttctc ctcgctgcaa gcgcaagacc cggatctcga
      961 cgtcgctctg ccctctccgc cctcggccgt ccccgtcccg gcggccgcgg gagatctgct
     1021 caatccgctg gagtcgttca tcgccaccac tcgtcccacg ctttcctaca gctacgccga
     1081 tgtcgatggc ctgctgtccg ctccaggtgt gcgcagcgag acctcgctgc aaaccatctc
     1141 agccaccttt tccgccgaac tcgtcgaaag ccttctcatc gaataccgcc cgatgtggat
     1201 cacctactcg aacgacgcgt tcgacg
//
LOCUS       DASPLT010001212         1451 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_15191, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001212 DASPLT010000000
VERSION     DASPLT010001212.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1451)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1451)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1451
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_15191"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..521)
                     /locus_tag="VEA63_12420"
     CDS             complement(<1..521)
                     /locus_tag="VEA63_12420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TAT-variant-translocated molybdopterin
                     oxidoreductase"
                     /protein_id="HYD84857.1"
                     /translation="MKRKFEHPAPSQRELTGPKYWRSLDELAETPGFKAQLEREFPEG
                     ASSIEGVDRRHFMKIMAASFALGGVGLAGCRRPERHILPFGKSVENVIPGLPQYYATA
                     MPLRKTAIPLLAETHQGRPTKIEGNPTYAPHGGSASLHAQASVLDLYDPDRARAHTRD
                     GNALDAAQVNELLA"
     gene            complement(518..1171)
                     /locus_tag="VEA63_12425"
     CDS             complement(518..1171)
                     /locus_tag="VEA63_12425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HYD84858.1"
                     /translation="MSYIFSKSANKLPLQIVIFLVVLAGIATAGVNYYMTPKYTRVGY
                     APVQPVPYSHALHVGQLGLDCRYCHSNVDKSGYANLPTAQTCMNCHNAVKPDSPLLAP
                     VRRSYETGEPVPWVKIHDLPDFAYFNHAVHVNRGVSCVECHGKINEMEVVEHAQPLSM
                     GFCLDCHRAPESRVRHPEDVFNLNSQTIAESEGIAAAQQFVHDWNITPPQSCTGCHR"
BASE COUNT          274 a          419 c          495 g          263 t
ORIGIN      
        1 gcgagcagtt cgttcacctg cgcggcatcg agcgcgttgc cgtcgcgcgt gtgcgcgcgg
       61 gcgcgatctg gatcatagag atcgagcacc gacgcctggg cgtgcagcga cgccgaaccg
      121 ccatgcggcg catacgtcgg attgccttcg atcttggtcg gccggccctg gtgcgtctcg
      181 gccaagagcg gaatcgcggt tttgcgcagc ggcatcgccg tggcgtaata ctggggcaaa
      241 ccgggaatga cgttctcgac cgacttgccg aacggcagaa tgtgacgctc cggacggcgg
      301 cagccggcca ggcccacgcc acccagggcg aaggaggccg ccatgatctt catgaagtgc
      361 cggcgatcga caccttcgat ggacgaagcg ccctcgggaa actcgcgctc gagctgggcc
      421 ttgaagcccg gagtttccgc gagttcgtcg aggctgcgcc agtacttggg accggtcagc
      481 tcgcgctgcg agggagcagg atgttcaaat ttgcgtttca tcggtggcag ccggtgcagc
      541 tttgcggagg cgtgatattc cagtcgtgca cgaattgttg ggcggccgcg atgccttcgc
      601 tctcggcgat cgtctgcgag ttgagattga agacgtcttc cggatggcgg acgcggctct
      661 ccggggcgcg atggcaatcg agacagaagc ccatgctcaa tggctgcgca tgctcgacga
      721 cttccatttc attgatcttg ccgtggcact cgacgcagct gacgccgcgg ttcacgtgca
      781 cggcgtgatt gaagtaagcg aaatcgggca ggtcgtgaat cttcacccac ggcacgggct
      841 cgccggtttc atagctgcga cggaccggcg cgagcagcgg gctgtcgggc ttgaccgcat
      901 tatggcagtt catgcaggtc tgcgcggtgg gcagattcgc gtaacccgac ttgtcgacgt
      961 tgctgtggca ataacggcag tccaaaccga gctgaccgac gtgcagcgcg tggctgtagg
     1021 gcacgggctg aaccggcgcg taaccgacgc gcgtgtattt cggcgtcatg taatagttga
     1081 cgcccgccgt cgcgatcccc gccagcacga cgaggaaaat cacgatttgc agcggcaact
     1141 tgttggccga tttcgaaaaa atatacgaca tgagccggag agcttcggtg gtccgaaccg
     1201 ggtttagacc gaatcgctac gccgggcgac ggcgcgcgat tcggaacgca gcgagaaagg
     1261 cttgggcgtg gccgcggcgg gcagctcgga agccgagttg cggacaacgg acttcgctag
     1321 cagccgcggc gccactccgg caaacgctgc caacgcgaac aatttgccga gaaaactctt
     1381 acgagatgga tcgtggctca cggggggtgg aaaaggttaa agtcgccgcc aaagaaggtg
     1441 tgcccgttgg c
//
LOCUS       DASPLT010001213         1346 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_260125, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001213 DASPLT010000000
VERSION     DASPLT010001213.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1346)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1346)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1346
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_260125"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..218)
                     /locus_tag="VEA63_12430"
     CDS             complement(<1..218)
                     /locus_tag="VEA63_12430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD84859.1"
                     /translation="MNLEITARACLRVLGLVLMLASVPVFAADAAAPASEAATLNPAA
                     PGNVDPIVSPTPMEFEAEGTGVHGQLVVA"
     gene            complement(386..808)
                     /locus_tag="VEA63_12435"
     CDS             complement(386..808)
                     /locus_tag="VEA63_12435"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HYD84860.1"
                     /translation="MKPRITVITIGVDDLERAVRFYRDGLGLQTDGIIGKEFEHGAVA
                     FFELEGGVRLAVWPRKSIAHDAGLTASGAAPTDFTLGHNVATAAEVDAVMEQAKNAGA
                     IVVKTAHKTFWGGYAGYFQDPDQHLWEVVWNPQLLPKS"
     gene            973..>1346
                     /locus_tag="VEA63_12440"
     CDS             973..>1346
                     /locus_tag="VEA63_12440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HYD84861.1"
                     /translation="MNAALSPSATSTARPLKPISLPPTPYQPQPYTGPSAAEVLALRK
                     QYLSPGLFLYYKQPIMIVEGRMQYVWDDTGKRYLDGLGGIVTVSVGHCHPHVVAAANK
                     QNETLQHSTTIYLQPNIARYAE"
BASE COUNT          259 a          478 c          367 g          242 t
ORIGIN      
        1 gcgacgacga gttgcccgtg cacgcccgtg ccctcggctt cgaattccat cggggtcggc
       61 gaaacgatcg gatccacgtt gccgggcgcg gcgggattca aagtcgccgc ctcgctcgcc
      121 ggcgcagccg catcggcggc gaaaaccggc acgcttgcca acatcaatac gaggccgaga
      181 acgcgcaggc acgcacgagc ggtgatttcg aggttcatag gagtgaaggt gatgcggcac
      241 atgacgtctg atgcgtcacg cccgccgcgg ccaagttccg cgatagaagc cagaacacgt
      301 ccgcttcctc cgcgacacgg caaattcatg gttattggaa acggccgatg aaggctgctc
      361 acggccagcg cacaacccaa acccgtcatg acttgggcaa caactgcgga ttccacacca
      421 cttcccaaag atgctgatcg ggatcctgaa aataacccgc gtagcctccc cagaaagtct
      481 tgtgcgccgt tttcacaacg attgcgcccg cgtttttcgc ctgctccatc actgcgtcca
      541 cctccgccgc ggttgcgaca ttgtgcccga gcgtgaagtc cgtcggggcg gctccgctcg
      601 ccgtcagccc cgcatcgtgc gcgatactct ttcgcggcca aaccgccaaa cgcactccgc
      661 cctccagctc gaagaacgcg actgcgccgt gctcgaactc ttttccgatg attccgtccg
      721 tctggagacc gagcccgtcg cgataaaagc gcacggcccg ctccaagtcg tcgacgccaa
      781 tcgtgatgac ggtaatcctc ggcttcatac gtgatcttac gataacgaca acggacgaga
      841 atggcgagcg cttgctgtca atcgcgccgg ccttccggtg aagacgagcc cgtcgttgcc
      901 gtcgcaccgc tcgcgttgtt catggtcctc ccggttctcg acttccgccg ctcgcgtggt
      961 taacccgtgt ccatgaacgc cgctctttcg ccgtctgcga cttccaccgc ccgcccgctg
     1021 aagccgatct cactgccgcc cacgccgtat cagccgcagc cttacaccgg cccctccgcc
     1081 gccgaggtcc tcgcgttgcg aaagcaatac ctcagccccg gccttttcct ctactacaag
     1141 cagccgatca tgatcgtcga gggccgcatg cagtatgtgt gggacgacac cggcaagcgt
     1201 tacctcgatg ggctcggcgg catcgtcacc gtcagcgtcg gccactgcca cccgcacgtc
     1261 gtcgccgcag cgaacaagca gaacgaaacg ctgcagcatt cgaccacgat ctacctccag
     1321 ccgaacatcg ctcgctacgc ggagaa
//
LOCUS       DASPLT010001214         1079 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_199666, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001214 DASPLT010000000
VERSION     DASPLT010001214.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1079)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1079)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1079
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_199666"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1079)
                     /locus_tag="VEA63_12445"
     CDS             complement(<1..>1079)
                     /locus_tag="VEA63_12445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD84862.1"
                     /translation="PCWKVRLTPMQVGTYRCRLLLTTGGRVVEIDAGSFEATTNSTRP
                     GFVRLARANPRYLERDDGSSFFVIGQNMLFPGGAGTYRMDKWLTSLARSGGNFIRTWW
                     CHAYMNLESRVSEKPMRQFGVYDLESAWRADHQLELSEALGVAVMPTIETQQFLRAGV
                     WWEHFTYNAANGGPLTTPADYFADTQAAQAFRNRLRYIVARWSYSTAIFSWQFWNEVD
                     SCNGYDPAKVGAWHRDMSRYLRSIDPNRHLINTNFGNLDGRTEVDGLPETELISTNLY
                     TRMDSAESSLWAARFMTGRYAKPYLLSEFGLGHYGRWAENDPDGIAMHDGLWGAAMGG
                     AAGGSLVWEWDKWVDPQNLYRHFAP"
BASE COUNT          213 a          350 c          330 g          186 t
ORIGIN      
        1 cggcgcgaaa tgacggtaga gattctgcgg atcgacccac ttgtcccact cccacaccag
       61 cgaaccgccg gcggctccgc ccatcgccgc gccccacaaa ccgtcgtgca tggcgatgcc
      121 gtcgggatcg ttctccgccc aacgaccgta gtggccaagg ccgaattcgc tcagcagata
      181 cggcttggcg tagcgtccgg tcatgaaacg cgcggcccag agcgatgact ccgcggagtc
      241 catgcgcgtg taaagattcg tcgagatcag ctccgtttcc ggcaggccgt cgacctcggt
      301 gcgtccatcg agattgccga agttcgtgtt gatgagatgc cggttcggat cgatcgaacg
      361 caggtaacgc gacatgtcgc gatgccacgc gccgaccttc gccggatcgt agccattgca
      421 cgaatcgact tcgttccaga actgccacga gaagatcgcc gtcgaatacg accagcgcgc
      481 cacgatgtag cgcagccggt tgcgaaaagc ctgcgccgct tgcgtgtccg caaaatagtc
      541 ggcgggcgta gtaagcggtc cgccattggc ggcgttgtag gtgaaatgtt cccaccacac
      601 gccggcacga agaaactgct gcgtttcgat cgtcggcatg acggcgaccc ccagcgcttc
      661 cgagagctca agctggtggt cggcgcgcca cgcgctttcc aaatcgtaga cgccgaactg
      721 gcgcatcggt ttttcggata cgcgactctc gaggttcatg taggcgtgac accaccacgt
      781 gcggatgaaa tttccgcccg agcgcgcgag gctcgtgagc cacttgtcca tccggtaggt
      841 tcccgcgccg ccgggaaaca gcatgttctg gccgatgacg aaaaacgacg aaccgtcatc
      901 gcgctcgaga taacggggat tcgcgcgcgc aaggcggacg aagcccggac gcgtgctgtt
      961 cgtcgtcgcc tcgaacgagc ccgcgtcgat ctcgacgacg cggccgccgg tcgtaagcag
     1021 caaacgacag cgatacgttc ccacctgcat cggcgtgagc cggaccttcc agcacggcg
//
LOCUS       DASPLT010001215         1117 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_157727, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001215 DASPLT010000000
VERSION     DASPLT010001215.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1117)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1117)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1117
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_157727"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            169..>1117
                     /locus_tag="VEA63_12450"
     CDS             169..>1117
                     /locus_tag="VEA63_12450"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HYD84863.1"
                     /translation="MLNISPLRRRWMILTIIFGAIVLNYIDRQIVSILKPTLKAEFAL
                     DDRGYAVLINVFTICYAAMYPVTGWLVDRFGAGRMMLAGVVTWSLACLGAGFTRSIGQ
                     LTFFRAMLGAAEPMVFPAHLRVVTTWFPASLRATANGVCVAGSSIGALIAPPVVAWLA
                     LNFNWHMAFIVPGAAGLVIGLAWWLIYRDPPAEIAKQAAGEGAPLVTPAFTWPQLWRR
                     RSLWGILLCRFVSDPVWYFCLFWLPGYLQESSGLTLAQVGMVGWIPFLVADIGGVGTS
                     IWSDRLVRGGMTPLRARKVMLTGMALLGPICVLTPHLPHT"
BASE COUNT          158 a          325 c          366 g          268 t
ORIGIN      
        1 aacaatctca cggacgttcc gacgatggcc gcgattttga aaaaactgac agtcgacctc
       61 gatgccggct ggaaagctct gcggtgatgt attcgctgcg ccgctctctg tcctagcccg
      121 cgcttttcgc aaccggcgca agcgcaacca accccttcgg tttttttgat gctgaatatt
      181 tccccccttc gccgccgctg gatgatcctc acgattattt tcggggcgat cgtgctcaac
      241 tacatcgacc ggcagatcgt atcgattctc aagccgacgt tgaaagccga gttcgcgctc
      301 gatgatcgcg gctacgcggt tctcatcaac gtattcacga tttgttacgc ggcgatgtat
      361 ccggtgaccg gttggctggt cgatcggttc ggtgcgggac gcatgatgtt ggcgggtgtg
      421 gtcacgtggt cgttggcgtg cctcggagcg ggattcacgc gttcgattgg gcaactgact
      481 ttctttcgcg cgatgctcgg agccgcggag ccgatggtgt ttcccgcgca tcttcgcgtg
      541 gtgacgacgt ggttcccggc gtcgctgcgc gcgacggcca atggtgtgtg tgtggccggc
      601 agttcaattg gtgcattgat cgcgccgccg gtcgtcgcgt ggctcgcgct caatttcaac
      661 tggcacatgg cgttcatcgt gcccggcgcc gctggattgg tgatcggctt ggcgtggtgg
      721 ctgatctatc gcgatccgcc ggcggaaatc gcgaaacagg cggccgggga aggcgcgccg
      781 ctcgtcacgc ccgcgttcac gtggccgcag ctttggcgtc gccggagtct atggggcatt
      841 ttgctctgcc gtttcgtcag cgatccggtg tggtatttct gcctcttctg gctgccgggt
      901 tatttgcagg aaagctccgg cctcacgctg gcgcaggtgg gcatggtcgg atggattccc
      961 tttctcgtcg cggacatcgg cggtgttggc acgtcgatct ggtccgaccg gctcgtgagg
     1021 ggcggcatga cgccgttgcg cgcgcgcaaa gtcatgctga ccggcatggc gttgctcggg
     1081 ccgatctgcg tgttgacgcc gcatctgccg cacacgg
//
LOCUS       DASPLT010001216         1561 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_15306, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001216 DASPLT010000000
VERSION     DASPLT010001216.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1561)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1561)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1561
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_15306"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..224)
                     /locus_tag="VEA63_12455"
     CDS             complement(<1..224)
                     /locus_tag="VEA63_12455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF721 domain-containing protein"
                     /protein_id="HYD84864.1"
                     /translation="MPDAPQPHIFSKLAEQLVGDLRGGLTPEDPRRSIKRPTQNLADV
                     VEQLLAKHQIGRPSAEQTIRDHWREIVGVA"
     gene            complement(346..>1561)
                     /locus_tag="VEA63_12460"
     CDS             complement(346..>1561)
                     /locus_tag="VEA63_12460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009509612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HYD84865.1"
                     /translation="PLCVFPIRYVARKVLRRAHQAQAQIGSLSAQVTENLAAAREVRA
                     FNLEDRATERFRATSRALFTAQLKIVKYAQGLNPVIEVISAIGIAVTITFAYRSGVTL
                     AALTSVLLALYMCYEPIKKLGYLNSDLKRGTAALDRLEPVLHANISITDPATPVSISR
                     IRGEIAFDRVTFAYDGGEAVLRDASVRIPAGTVCALVGPSGAGKTTFANLVPRFYEVK
                     SGAVTIDGHDVRSFRLRDLRRNIAIVSQDPVLFNDTIYNNLLLGREDATRAEVIAAAS
                     DAHADEFIRSLPQGYDTMVGERGALLSGGQKQRIALARAFLRNAPILILDEATSALDS
                     QSEQLIQEALKKLVVGKTVLIIAHRFSTIRDASMILVFDRGELIATGSHTEVYAASPL
                     YKNLYDGQTVSA"
BASE COUNT          271 a          389 c          603 g          298 t
ORIGIN      
        1 ttggcgacac cgacgatctc gcgccagtga tcgcggatcg tttgctcggc ggaagggcga
       61 ccgatctggt gcttggcgag caattgctcg acgacgtccg cgaggttttg ggtcgggcgt
      121 ttgatcgaac gacggggatc ctcgggcgtc agaccgccgc gcagatcgcc gacgagctgc
      181 tcggcgagtt tgctgaagat gtgcggctgc ggcgcgtcgg gcatggcgga cggtggatcg
      241 gtcggaattg aaccacgaat ggacacgaag ggacacgaat tcgatccagg atggttcggc
      301 tattcgtgtg agttcgtgtc cattcgtggt tgaagacttc gggttttacg cgctcacggt
      361 ctggccgtcg taaaggtttt tgtagagcgg gctcgcggcg tagacttcag tgtgactgcc
      421 ggtggcgatc agttcgccgc ggtcgaagac gaggatcatc gacgcgtcgc ggatcgtgct
      481 gaaacggtgc gcgataatca ggacggtttt acccacgacg agtttcttga gggcttcctg
      541 gatgagctgt tcgctctgcg agtcgagggc ggaggtggct tcgtcgagga tgagaatcgg
      601 ggcgttgcgg agaaacgcgc gggcgagggc gatgcgctgt ttctgtccgc cggagagaag
      661 cgcgccacgc tcgccgacca tcgtgtcgta accctgcggc agtgagcgga tgaactcgtc
      721 ggcgtgggcg tcggacgcgg cggcgatgac ttcggcgcgc gtggcgtctt cgcggccgag
      781 gagaaggttg ttgtagatgg tgtcgttgaa gagcaccgga tcttgggaaa cgatcgcgat
      841 gttgcggcgg aggtcgcgca ggcggaagga ccgaacgtcg tggccgtcga tggtgacggc
      901 gccggatttt acttcgtaga aacgcggcac gaggttggcg aacgtggtct tgccagcgcc
      961 gctgggacca acgagcgcgc agaccgtgcc ggcggggatg cggaccgagg cgtcgcgcag
     1021 aaccgcttcg ccaccgtcgt aagcaaacgt gacgcggtcg aacgcgatct cgccgcgtat
     1081 tcgcgagatg gagacaggcg tggcgggatc ggtgatggaa atgttggcgt ggaggacggg
     1141 ctcgaggcgg tcgagggccg cggtgccgcg tttcaagtcg gagttgagat agccgagttt
     1201 tttgatcggc tcgtagcaca tgtagagtgc cagcagcaca ctggtgagag cggcgagggt
     1261 gacgccactg cggtaggcga aggtgatcgt gaccgcgatg ccgatggcgg agatgacctc
     1321 gatgaccggg ttcaggccct gcgcgtattt gacgattttg agctgcgcgg tgaagagcgc
     1381 gcgcgaagta gcacggaaac gctcggtggc gcggtcttcg aggttgaagg cgcgcacctc
     1441 gcgggcggcg gcgagatttt cggtgacttg ggcggagagc gatccgattt gcgcctgggc
     1501 ttggtgggcg cggcggagga ctttgcgcgc aacgtagcgg atcgggaaaa cgcacagcgg
     1561 c
//
LOCUS       DASPLT010001217         1041 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_218892, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001217 DASPLT010000000
VERSION     DASPLT010001217.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1041)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1041)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1041
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_218892"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..709
                     /locus_tag="VEA63_12465"
     CDS             <1..709
                     /locus_tag="VEA63_12465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="deoxyguanosinetriphosphate triphosphohydrolase"
                     /protein_id="HYD84866.1"
                     /translation="ARSFVFGGAAIPAELQVDEQLNVFKSIECQIMDWADDAAYSLND
                     IVDGVKAGFLTRERIERWAAGEAIDSPRQHLLDTLFDAITADKLESVFSQKIGAFITA
                     CRLRERENFMAARTNRYRYELVVSSEVQNEAFFFKKLANDIIFESPQLQQIEHKARRV
                     MFDLWDSAWRNYVEKNVRVISILPPRVGKMIEAETTHAGKARQICDYLAGLTDGMIVR
                     TYRRLFDPVFGSLRDLS"
     gene            complement(736..>1041)
                     /locus_tag="VEA63_12470"
     CDS             complement(736..>1041)
                     /locus_tag="VEA63_12470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HYD84867.1"
                     /translation="ARAADRIFGLLPDDQAKEFRALWDEFEAKATPEAKFATAVDRFQ
                     PMLLNSLTQGAAWQRHGITHDRVVARNCHIADGCKHLWSYAEQMLEEVVKAGHLAKT"
BASE COUNT          189 a          295 c          344 g          213 t
ORIGIN      
        1 ggcgcggagc ttcgtgtttg gcggcgctgc gatcccggca gagctgcagg tcgacgagca
       61 gctgaacgtt ttcaagagca tcgaatgcca gatcatggac tgggcggatg acgcggcgta
      121 ttcgctcaac gacatcgtcg acggtgtgaa ggccggattc ctcacgcgcg agcggatcga
      181 gcgctgggcg gccggcgagg caatcgattc accgcggcag cacctgctcg atacgttgtt
      241 cgatgcgatc acggcggata aactggagag cgttttctcc cagaaaatcg gcgcgttcat
      301 caccgcgtgc cgcttgcggg agcgggagaa tttcatggcg gcgcgcacga atcgttatcg
      361 ctacgagctg gtggtttcga gcgaggtgca gaacgaagcc ttttttttca agaagctggc
      421 caacgacatc attttcgaga gtccgcaact gcagcagatc gagcacaagg cacggcgcgt
      481 gatgttcgat ctctgggatt ccgcgtggcg caactacgtc gagaaaaacg tgcgtgtgat
      541 cagcatcctg ccaccacgcg tgggcaaaat gatcgaggcc gaaaccactc acgccggcaa
      601 agcgcggcag atctgcgatt atctcgcggg tctgacggac ggcatgatcg tgcgcacgta
      661 tcgccgcttg ttcgatccgg tgtttggttc gcttcgcgat ttgagctgag cggtggcggg
      721 cagctctccc gcggctcagg tttttgcgag gtggccggct ttcaccacct cttcgagcat
      781 ttgttcggcg tagctccaga ggtgtttgca gccatccgcg atgtgacagt tgcgcgcgac
      841 gacgcggtca tgcgtgatgc cgtggcgttg ccacgcggct ccctgcgtga gggagttcag
      901 cagcatcggt tgaaagcggt ccaccgcggt ggcgaacttc gcttcgggcg tggccttcgc
      961 ctcgaactcg tcccaaaggg cgcggaactc cttcgcctgg tcatccggca ggaggccgaa
     1021 gatgcgatcc gcggctcgtg c
//
LOCUS       DASPLT010001218         2079 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_54098, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001218 DASPLT010000000
VERSION     DASPLT010001218.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 2079)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2079)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2079
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_54098"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            264..1487
                     /locus_tag="VEA63_12475"
     CDS             264..1487
                     /locus_tag="VEA63_12475"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HYD84868.1"
                     /translation="MPFKALNLSEPILRGVQAAGYTDPTPIQLRAIPVVLGGGDLIGS
                     AQTGTGKTAAFALPILSRLGAHGRARVLVLEPTRELAAQVETAFRDFARFTDLRTVAV
                     FGGVGYGHQRSELKRGVDIIVATPGRLMDYLKDRTLSLQDISILVLDEVDRMLDMGFL
                     PVVKDIIARCPRERQTLFFSATVPPEIQAVASFALRNPARIEVGINRSVNESVKHALY
                     PVAAEQKFDLLEALLSKTDFDSVLVFSRTKHGADKIARRLKAANHSVAVLHANRSQNQ
                     RIEALAGFKSGKYEVMVATDIAARGIDVAGVSHVINYDVPEKPEDYVHRIGRTGRAQA
                     VGDAFTLVTPENANDIRDIQRFIGAKIPELRLEGFDYKPIVARPVDSAPQRPRGSLPG
                     QIRHKGSFNRFRGRR"
     gene            1685..>2079
                     /locus_tag="VEA63_12480"
     CDS             1685..>2079
                     /locus_tag="VEA63_12480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HYD84869.1"
                     /translation="MVPPPVVGLASLSRVILVGQAPGDKEPKFGRPFAWTAGKTLFGW
                     FATLGLTEEQFRSRVYMAAVARCFPGKATGGGDRVPSPDEVKRCSRHMETEIRILRPR
                     LVIPVGKLAIAQLYPDLDKLTEIIGEQRRG"
BASE COUNT          345 a          748 c          602 g          384 t
ORIGIN      
        1 cggcggcggc ggcggtggtc gcggcttcgg cggcggcggc ggtcgtggcg gcttcggcgg
       61 cggcggtcgc ggtggcgatc gcgaccgtgg cgagcgtcgc tactaatcga atcgttctaa
      121 cgaactgatt tttctgaagg acggagctcc ttgtgggctc cgtccttttt tgtctcttta
      181 aatataccgg tagggcgagc cgtccccggc gagttgcact ttcgcgactc tcccagaacg
      241 cttcgtccgc gtttcctgtt ctcatgcctt tcaaagctct taatctttcc gagccgattc
      301 tccgcggcgt ccaagccgcc ggctacaccg atccgacgcc aatccagctt cgcgccattc
      361 ctgtcgtcct cggcggcggc gacctcatcg gctccgccca aaccggcacc ggcaaaaccg
      421 ccgccttcgc gctgccgatt ctctcgcgcc tcggcgctca cggacgcgcg cgcgtcctcg
      481 tcctcgaacc cacgcgcgag ctcgccgccc aagtcgagac cgcgttccgc gacttcgcac
      541 gtttcaccga tctccgcacc gtcgccgttt tcggcggcgt cggctacggc caccaacgct
      601 ccgaactcaa acgcggcgtc gacatcatcg tcgccacgcc cggtcgcttg atggactacc
      661 tcaaggaccg cacgctcagc ttgcaggaca tctcgattct cgttctcgac gaagtcgacc
      721 gcatgctcga catgggtttc ctcccggtcg tcaaagacat catcgcgcgc tgcccgcgtg
      781 agcgccagac gctctttttc tccgcgaccg tcccgcccga gattcaagcc gtcgcctcgt
      841 tcgccctgcg caatcccgcg cgcatcgagg tcggcatcaa tcgctccgtc aacgagtccg
      901 tcaaacacgc gctctatccc gtcgccgccg aacagaagtt cgatctcctc gaggcgctcc
      961 tgagcaaaac cgatttcgac agcgtgctcg ttttctcgcg cacgaaacac ggcgccgaca
     1021 agatcgcgcg tcgcctgaaa gccgcgaatc attccgtcgc cgttctccac gcgaaccgtt
     1081 ctcaaaacca gcgtatcgaa gcgctcgccg gtttcaaatc cggcaaatat gaagtgatgg
     1141 tcgccaccga catcgccgcc cgcggcatcg atgtcgccgg cgtgtcgcac gtgatcaact
     1201 acgacgttcc ggaaaaaccc gaagactacg tgcaccgcat cggtcgcacc ggccgcgcgc
     1261 aagccgtcgg cgacgcgttc acgctcgtca cgccggaaaa cgcgaacgac atccgtgaca
     1321 tccagcgttt catcggcgcg aagattccgg agctccgtct cgaaggtttc gactacaagc
     1381 cgatcgtcgc ccgtcccgtg gacagcgcgc cgcaacgccc gcgcggcagc ctccccggcc
     1441 agatccgcca caaaggaagc ttcaaccgct tccgcggccg ccggtaagcg tttaaaacgt
     1501 ctccggcgca gcaccacccg tggcgcggtc aacctgaccg cgctacgttt acgttcacgc
     1561 tcgcgatcgc gagtttcgga tggcagcagc cgatgactga tggcagggaa atgaggtgcc
     1621 agttactgtt gcctcgctcg agaaacattt caccgccttg cgggcctgcc ggcgttgccc
     1681 gcgcatggtc ccgccgcccg tcgtcgggct cgcctcgctc tcccgcgtga tcctcgtcgg
     1741 tcaggcgcct ggcgacaagg agccgaagtt cggccggccg ttcgcgtgga ccgccggcaa
     1801 gacgctgttc ggctggttcg cgacgctcgg cctgaccgag gagcagtttc gctcgcgcgt
     1861 ctacatggcg gccgtcgcgc gctgcttccc gggcaaggcc accggtggtg gcgatcgcgt
     1921 gccgtcgccc gatgaagtga agcgctgcag ccgtcacatg gagaccgaga tcaggatcct
     1981 gcggccgcgg ctcgtcatcc cggtcggcaa gctcgcgatc gcgcagctgt accctgatct
     2041 cgacaagctc accgagatca tcggcgagca aagacgcgg
//
LOCUS       DASPLT010001219         1480 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_199719, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001219 DASPLT010000000
VERSION     DASPLT010001219.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1480)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1480)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1480
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_199719"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..480)
                     /locus_tag="VEA63_12485"
     CDS             complement(<1..480)
                     /locus_tag="VEA63_12485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HYD84870.1"
                     /translation="MNHQSTPLRRRATLFALALFGAGGIAWSAATAGDAKSSVSVKVD
                     EKPIASDASSPLGQSYAPIVKRVAPSVVKVLVAEKAKSVPVGELPPFFNHPGFREFFG
                     EDFGRQFGGRGGRGMMRQPPSQGLGSGVIVSSDGFILTNSHVVQGADTIEVSLNDGRE
                     "
     gene            complement(620..>1480)
                     /locus_tag="VEA63_12490"
     CDS             complement(620..>1480)
                     /locus_tag="VEA63_12490"
                     /inference="COORDINATES: protein motif:HMM:NF019229.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRTAC1 family protein"
                     /protein_id="HYD84871.1"
                     /translation="ETGTPRLLEAEYDDGVLVPVRGRSTMERVMPWIARKMPTFKRYA
                     AASLADIFGEEAIEKAERHAVTELRSGVFLSRASVGGYAFEPLPRMGQIAPIFGVAAG
                     DFDGDGRADIYAVQNSYAPIPEVGRFAGGLSQLLRGDGKGGFAVADVAETQLIVPGDA
                     KGLAVLDVDRDGWPEFLVTRTTNEALVFGNTGVAGRTMRGVILRGRGGNPTAVGARVT
                     AVLADGSTQTAEVSAGVGYLAQSSATLFFGAPDENPLVRLRVRWPWGEATEVAVPAGA
                     GLLRISAPPE"
BASE COUNT          275 a          575 c          388 g          242 t
ORIGIN      
        1 ctcacggccg tcgttcagcg acacttcgat cgtgtccgcg ccttgcacga cgtggctgtt
       61 cgtcaaaata aagccgtccg agctcacgat cacgcccgag ccgagacctt gcgacggcgg
      121 ctgacgcatc atcccgcgac cgccgcgacc gccaaactga cggccgaagt cctcgccaaa
      181 aaattcccgg aatcccggat ggttgaaaaa cggcggcagc tcgccgactg gcacgctctt
      241 cgctttttcc gcgacgagca ccttcacgac gctcggcgcc acacgcttca cgatcggcgc
      301 gtaactctga cccagcggcg aagacgcgtc cgacgcgatc ggcttttcgt cgaccttaac
      361 cgagacagaa gatttggcgt cgcccgcggt cgccgcggac cacgcgatgc cgcccgcccc
      421 gaacagggcg agcgcgaaca gcgtcgcgcg gcggcggaga ggagtggatt gatggttcat
      481 agtgcagaaa tggagttgag gttgacgttc ggttttagaa cgccgccacc ttcgcacacc
      541 cacccttctc cctcctttcc gaatccttac atttttgaag ggaacacggc caccctgcgc
      601 ccgacgctcg gctctcacct cactccggcg gcgcggaaat ccgaagcaat cccgctcccg
      661 ccggcaccgc cacttcagtc gcttctcccc aaggccagcg cacgcgcagc cgcacgagcg
      721 gattctcgtc cggcgcgcca aaaaacaacg tcgcactcga ctgcgcgaga taacccaccc
      781 ccgcgctcac ctcggccgtt tgcgtcgagc cgtcggctaa aaccgccgtg actcgcgcgc
      841 caaccgccgt cggatttccg cctcgcccgc gcaaaatcac cccgcgcatc gtccgcccgg
      901 ccacgcccgt attaccgaac accagcgctt cgttcgtcgt gcgcgtcacc aaaaactccg
      961 gccagccgtc gcgatcgacg tccaacaccg ccagcccctt cgcatcaccc ggcacgatca
     1021 actgcgtctc cgccacatcg gcgaccgcga atccgccttt gccgtcgccg cgcaacaact
     1081 ggctgagccc gcccgcgaaa cgtcccacct ccggaatcgg cgcataggaa ttctgcaccg
     1141 cgtaaatatc cgctcgcccg tcgccgtcga aatcccccgc cgccacgccg aaaatcggcg
     1201 cgatctgccc catccgtggc aacggctcga acgcatatcc gccgacactt gccctactga
     1261 gaaacacccc gctgcgcagc tccgtcaccg catggcgctc cgccttttca atcgcttcct
     1321 cgccgaagat atccgcgagc gacgccgcgg cgtagcgctt gaaggtcggc atctttcgcg
     1381 cgatccatgg catcacgcgc tccatcgtcg agcgtccgcg caccggcacg agcacaccat
     1441 cgtcgtattc ggcttcgagc agccgcggcg ttcccgtctc
//
LOCUS       DASPLT010001220         1070 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_95709, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001220 DASPLT010000000
VERSION     DASPLT010001220.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1070)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1070)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1070
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_95709"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..622
                     /locus_tag="VEA63_12495"
     CDS             <1..622
                     /locus_tag="VEA63_12495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD84872.1"
                     /translation="DPPLAAKIEPGVSSLLPLYFAGKPDVMAQEVERLRGEYLRLGVG
                     VAEKNYVQLLGEPAATVWLKAVLLLEYAAGLANKETMEIHTITADGSVLAGDKFFAFA
                     GFINEEARWHDYNIGRKNARHWLKHGGGGLFPINASFAETLPEVDEGLGGWEIGHFDR
                     KKRENLMAQARDRSGIVMKEMGVNAVLRGAIDMFVVRPRLKKMLGL"
BASE COUNT          184 a          276 c          402 g          208 t
ORIGIN      
        1 ggacccgccg ctcgcggcga agatcgagcc gggagtgtcg tcgctgctgc cgttgtattt
       61 tgccggaaag ccggacgtga tggcgcagga agtcgaacgg ttgcgcggcg aatacctgag
      121 gctcggcgtc ggcgttgcgg aaaaaaacta tgttcagttg ctcggcgagc cggcggcgac
      181 ggtgtggttg aaagcggtgt tgctgctcga gtatgccgcc gggctggcga acaaggagac
      241 gatggagatt cacacgatca cggccgatgg cagcgtgctt gcgggggaca agttttttgc
      301 gttcgcgggc ttcatcaacg aagaggcgcg ctggcacgac tataacattg gtcggaaaaa
      361 cgcgcggcac tggctgaagc acggcggcgg aggattgttt ccaatcaacg cgagttttgc
      421 ggagacgctg ccggaggtcg acgagggcct cggcggctgg gagattggcc acttcgaccg
      481 caagaagcgc gagaatctga tggcccaggc gcgcgatcgg tccggcatcg tgatgaaaga
      541 gatgggcgtg aacgccgtct tgcgtggcgc gatcgacatg ttcgtggtgc gaccgcggtt
      601 gaagaaaatg ctggggttgt aaggcgtgcg atagaggaaa tttggtggct gcgtcgggcg
      661 aaccgtctcg gagcggcccg cccggcggcg aactgtcccg gtgagttgcg gctcggcggc
      721 gacggccgcc ctgccgctcg accaagctcg atgggactcg tggagtggtc tgcgagcgga
      781 cgcgcgcgag cccgatgggg acatccggct tcaccttcaa gttaacggtg gctggacagc
      841 gcgatcggga gcgcggcagg ctgcggacgc gctgcgcgtt tttgcagcgc ggttcagcaa
      901 gacgcgtgtt catgattatc cgccgggtcc ctatgcgatt ttgggctgcc gcttggtgcg
      961 cggtggcggg cgcggtgttg tttcaatttt tcggcaatgc aacgcgcggc tacgttgata
     1021 cgccctcggt gttctggtgg tgggtgagtc agtggatcaa ccctcaggcg
//
LOCUS       DASPLT010001221         1247 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_179365, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001221 DASPLT010000000
VERSION     DASPLT010001221.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1247)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1247)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1247
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_179365"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..352
                     /locus_tag="VEA63_12500"
     CDS             <1..352
                     /locus_tag="VEA63_12500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009510961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HYD84873.1"
                     /translation="YVEKAALEWYQEHPNELHVKYPDQPKQIWRRGNGYNGAAAQKFT
                     RIPAGHPERYLEAFGNIYREAFRAITAEIEGQPQPKDLDFPTIDDGLEGMAFIETAVK
                     SSAQGAKWVKFPKL"
     gene            complement(725..>1247)
                     /locus_tag="VEA63_12505"
     CDS             complement(725..>1247)
                     /locus_tag="VEA63_12505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD84874.1"
                     /translation="AATTERLGKYRTQQSQVRKNDEYQALGHEIETTEAAIGKLEEQD
                     LEVMYAIDEAKKKFADAEAVLKANISGHEGRIKTLREREMNLTAELTAAQAEVAAART
                     PVSEPALRLYDRVSARNMPAVVAIRGGKCAGCHLKVSSEVESASRGKSADAGAGPQLA
                     TCDQCGRIVFWEG"
BASE COUNT          244 a          417 c          340 g          246 t
ORIGIN      
        1 ctacgtcgaa aaggccgcgc tcgagtggta tcaggaacac ccgaacgaac tgcacgtgaa
       61 ataccccgat cagccgaaac aaatctggcg ccgcggcaac ggctacaacg gcgccgccgc
      121 gcaaaagttc acgcgcatcc ccgccggcca tccggaacgt tatctcgaag cgttcggcaa
      181 catctaccgc gaagccttcc gcgccatcac cgccgaaatc gaaggccagc cgcagccgaa
      241 agacctggat ttccccacca tcgacgacgg cctcgaaggc atggccttca tcgaaaccgc
      301 cgtgaagagc agcgcccaag gcgcgaagtg ggttaagttt ccgaagctct aaaaccgcga
      361 gaagcactcg ccgctgatcg cccgaaggtg aacgctcgaa ggtggagccc gacgtccccg
      421 tcgggttggt ttgtaggcga acgcccttag ccgcagcaat tcagttggaa accaccgagg
      481 gacacggatt gaaaaccctt tccgcagcga aagggtcgtt tgaaaaggac tcactctttg
      541 gtgaaaagaa acatgtcgcg ttaggttcct gaacaacgag gccgtatccg tgtttatcgg
      601 tgttcatccg tggttgaatt gcatggttcc ggcttagccc gccggaacgg cgggctccac
      661 ctttggatcg attcacgagc gtgactccac gttcacgcgt tggatcgtgt cgcccgccgc
      721 cagctcaccc ttcccaaaac acgatccgtc cacactgatc gcacgtcgcg agttgcggcc
      781 ctgcgccagc gtccgcgctt ttgccgcgtg acgccgattc gacttcgctc gaaaccttca
      841 agtggcaccc cgcgcatttt ccgccacgga tcgccaccac ggccggcatg ttgcgcgcag
      901 agacccgatc gtagaggcgc aaggcgggct cgctcaccgg cgtccgcgcc gcagccacct
      961 ccgcctgcgc cgccgtcagc tccgccgtca aattcatttc gcgctcccgc agagtcttga
     1021 tgcgtccttc atgacccgag atgttcgctt ttaaaaccgc ctcggcatcc gcgaatttct
     1081 tcttcgcctc gtcgatcgca tacatcacct cgagatcctg ctcctcgagt ttgccgatcg
     1141 ctgcctcggt ggtctcgatc tcgtggccga gcgcctgata ttcgtcgttt ttacgcactt
     1201 gcgattgctg tgtgcggtat ttgccgagcc gctcggtggt cgccgcg
//
LOCUS       DASPLT010001222         3016 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_157771, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001222 DASPLT010000000
VERSION     DASPLT010001222.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 3016)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3016)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3016
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_157771"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>3016)
                     /locus_tag="VEA63_12510"
     CDS             complement(<1..>3016)
                     /locus_tag="VEA63_12510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD84875.1"
                     /translation="VTRAIALQSALAAPESLLIGSLGDVGLLGSHRGETVADVRARVG
                     QAPLIWIHGAHGVGKSTLCKLVANAYGGEWLAIDLKPLAGKSDAILTAWRSLHQIVLM
                     NGMPDGVIIDDFDQDAVLALWPRLKAFARILFERGGRLLLNSHQAPSPARIAELGLAA
                     DVIIQAPYFTQAEITDLLRQAPAPLEDEREPWAFFIRGATGGGHPVLVSAKVASLRSR
                     AWPKAALLEDVGPQSSDAVRATRQEARQILLREIGLLGEERARAAGRMLRRIASIVDR
                     VDEALILRLAEIDPDIEASTDLLALLKGSWLELMPGGDLRVSPLLGDIADDVSDDDKK
                     TWRCTASIHWMAGRVLNERTIPLIFWNAYLGRQEWVLGKFCQVLFTQSRQALGGAAAL
                     LSPIMVFSIETPIYSGHALINANLRLLQFEVAETLEHGAKAAAIAKQLIADINAIEIS
                     EVRPMMTAVACSKVLMAAHAETEPKDVVRYALLLRQSLATLQASGEEDLLARWNAVVA
                     EFDPSLDQASFLFANSISKIATSADFLGLIEALDPISEADRNIFLDASSVAFDGLSVF
                     VNSGWSRDQLEDRDMQAALETYQKAASIAERWKRPDIMIQLAAAISVLFDEGLKNETA
                     ALAAIDEAIAAFGPDQALIRQKAKVFGHYDRDEEAADLIVSVEDSVGGDSFFDRALAL
                     RDGATSAARAGRFGDAARLYTKAAAAVAEKGVRRALLAGLNIDRAMATWDSGDRAGAL
                     GQLADVFDQLAAFEPQSSRQAERAHQFGRGVGGLFAFDVGIIPGAQRPNIAYGGASAL
                     STDDEPLLDADLKPLADNWRILTLVERASELDIGIGARSRAAQTGPGLVATEFLFFLD
                     DYARSLRRGDLGEILVMGVKAASALVTATEATAGGDGMVRADASALGAIPLAALLARS
                     ESKEALYRIPTDVLIYARLRGAWSERRVDELQRACEHVFGGSDPWFAPFFEVAHGRRT
                     VAAQAPMPVWGAAAAAMSEATVRDDPGQRFKRD"
BASE COUNT          606 a          987 c          886 g          537 t
ORIGIN      
        1 atccctcttg aaccgctggc ctgggtcatc gcgaacggtc gcttcactca tcgcggcggc
       61 agcggcaccc cacactggca tcggcgcttg cgcggcaact gtgcgccgcc cgtgagccac
      121 ctcgaagaac ggcgcgaacc aaggatcgct tccgccaaag acgtgctcac acgcccgctg
      181 gagttcgtct actctgcgct ctgaccaagc accgcgaaga cgtgcataga ttaaaacgtc
      241 cgtggggata cgatagagcg cctctttgct ttcggagcgt gcaagaagcg ctgcgagcgg
      301 gatggcgcca agcgccgacg cgtcggcgcg caccatgccg tcgccgccgg ctgtcgcttc
      361 tgtcgccgtc accagcgccg aagccgcttt cacacccatc accaggatct cgcctagatc
      421 gccgcgtcgc agagagcgcg cgtagtcgtc aaggaagaag agaaactcgg tcgcgaccag
      481 ccctggcccc gtctgcgcgg cgcgtgaacg tgcgccgatg ccaatgtcga gttcgctcgc
      541 gcgttcaact agcgtcagaa tacgccaatt gtcggcaagc ggttttagat cggcatcaag
      601 caacggctca tcgtcggtag agagcgcaga agcgccgcca taggcaatgt taggccgttg
      661 cgccccgggg ataatgccga catcgaaggc aaacagccct ccgacgccgc ggccgaattg
      721 atgcgcgcgc tcggcttggc gcgaactttg cggctcgaac gcagccagtt gatcaaacac
      781 atctgcaagc tggccaagcg cgccagcgcg atcgccggag tcccaggtgg ccatggcgcg
      841 atcaatgttg agccccgcca gcagcgcgcg ccgcacgccc ttctcagcaa cggccgctgc
      901 ggccttagtg taaagacgcg ctgcgtctcc aaacctgccg gcgcgcgcgg ccgaggttgc
      961 cccatcacgc agcgccagcg cgcgatcgaa aaatgaatcg ccgccaactg agtcttccac
     1021 gctcacgata agatctgcgg cttcctcgtc gcgatcataa tgaccaaaca ctttggcctt
     1081 ctgccggatc aaagcttgat cgggcccaaa tgctgcaatg gcctcgtcga tagccgccag
     1141 cgccgctgtc tcgttcttca acccttcatc gaacaagacc gagatcgccg cggcgagctg
     1201 gatcatgatg tcgggccgct tccagcgctc agcgatgctt gcggcctttt gataggtttc
     1261 aagcgctgcc tgcatgtcgc gatcttcgag ctgatcgcgc gaccagccgc tgttgacgaa
     1321 aacggagagg ccgtcgaagg caaccgacga cgcatcgagg aagatgttgc gatcggcttc
     1381 cgagatcgga tcgagcgctt cgatcagccc caaaaaatcc gcggatgttg cgattttgct
     1441 gattgagttg gcgaacagaa agctcgcttg atctaggctt ggatcaaact ccgccaccac
     1501 cgcattccag cgcgccagga gatcctcttc gcctgaggcc tgcaacgttg ccagcgattg
     1561 gcgcagcaac agggcatacc ggacgacgtc tttgggctcg gtctcggcgt gagcggccat
     1621 caacaccttc gaacacgcca ccgccgtcat catcgggcgg acttcgctga tctcgatggc
     1681 attgatatct gcgataagct gttttgctat agcagcggcc ttggcgccgt gctccagcgt
     1741 ctcggctacc tcgaactgaa gcaggcgaag gttagcgttt attagcgcat gcccgctgta
     1801 gatcggcgtc tcaattgaaa acaccatgat gggcgacaac aaagcggcgg caccgccaag
     1861 cgcttgacgc gattgggtga agagcacttg gcagaacttt ccgagcaccc attcctgacg
     1921 ccccagatac gcgttccaga aaatgagagg gatggtgcgc tcattcagta cccgacccgc
     1981 catccagtga attgaggcgg tgcaacgcca ggtcttcttg tcgtcgtcgc tcacatcgtc
     2041 ggcgatgtcg cccaagagcg gcgacacgcg cagatcgccg cccggcataa gctcaagcca
     2101 cgaacccttg agcagcgcca agaggtcggt tgacgcttca atgtcagggt cgatttccgc
     2161 caagcgcaga atgagcgcct cgtcgacacg atcgacgata ctggcaatgc ggcgcagcat
     2221 gcgccccgcc gcacgcgccc gttcttcacc cagaaggcct atttcgcgca gcaaaatctg
     2281 gcgcgcctcc tgacgcgtcg cccgcaccgc atcgctgctt tgcgggccga catcttccag
     2341 cagcgctgct ttcggccacg cgcgcgatct caagcttgcg accttcgcgg acaccagaac
     2401 cggatggccg ccccctgtcg cgccgcggat aaagaacgcc cacggctcgc gctcatcttc
     2461 aagcggcgcg ggcgcttgtc gcagcagatc agtgatttca gcctgagtga agtatggcgc
     2521 ctggatgatg acgtcggccg cgaggccaag ttcggcgatc cgcgccggcg acggtgcttg
     2581 atgcgaattc agcaacagcc gtccgccgcg ctcgaacaag atgcgcgcaa acgcctttaa
     2641 cctcggccaa agcgcgagca ccgcgtcttg gtcaaaatcg tcgatgatga cgccatccgg
     2701 catcccgttc atcagaacga tctgatgcaa cgaacgccaa gcggtcaaaa tggcgtcgct
     2761 cttgccggcg agcggcttca gatcgatcgc aagccattca ccgccataag cattggccac
     2821 tagcttgcac aaggttgact tgcccacccc gtgggcgccg tggatccaga tcaacggcgc
     2881 ctgccccacc ctagcgcgga catcggcgac ggtttcgccg cggtggcttc ccaggagacc
     2941 gacgtcgcca agagagccga tgagcaggct ttcgggcgcg gcgagcgcgc tctgtagcgc
     3001 aattgcgcgg gtgacg
//
LOCUS       DASPLT010001223         2115 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_138424, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001223 DASPLT010000000
VERSION     DASPLT010001223.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 2115)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2115)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2115
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_138424"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(103..483)
                     /locus_tag="VEA63_12515"
     CDS             complement(103..483)
                     /locus_tag="VEA63_12515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009509875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HYD84876.1"
                     /translation="MIKKLLHTRMRVNDLERTVKFYENALGLKVARRHTSPRGAQLVF
                     LETPNSDEEIELCYLPNSESVKVQPDLMHLAFEVEDLNAFAEGLKKKGYALSDGPTET
                     GSGSMIAFIDAPEGYEVELIQRAK"
     gene            725..>2115
                     /locus_tag="VEA63_12520"
     CDS             725..>2115
                     /locus_tag="VEA63_12520"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HYD84877.1"
                     /translation="MPVSPPSSRASLAEGPATRTPVIDRLARWHPLLLLLGCCIAATA
                     LCTWFYSREAKHAEAVLRERETVRVGAFGHQLTENFASKAARLRTLAASQTLGEFLEH
                     GGREYLDRFARDAWVLHLHHDDIDQISYIDNNGREQMRVDNDEGILPATAMRDRANLP
                     LFQEALHLQPGEVRRSRIDLDEENGIVTTPWKPRLRFAAAVFDDQGERRGVIIINFLA
                     QQLVEHFANFYPVYSHRLRLLNDKGYWLRTANPADEWGFVFADRNDRTLARQAPALWR
                     RVSTEAQGQARYNGGLLTWRRFDPASTAADTRPGGASFLLVGSEVSADEWTATFAQLR
                     QTFTLVGAALLIVTLAITWLYQRQRREAERRRETDELNAAILHSAGAGVITLDASGTV
                     RSLNVTAQRLLGWDAAEIVGRKNASVLYDRDAMKAFARETGAAMNRHFRHGLEALAAK
                     VLAADGPIEREWI"
BASE COUNT          362 a          809 c          566 g          378 t
ORIGIN      
        1 cccgtgcttc aggccgtggg aggggcttta cgccccgaca cacgccagca gcgccaagca
       61 cggtcagatt gaccgcgcta ccttgcggcc atcgcgcggc gactacttcg cccgctgaat
      121 caactccacc tcgtagccct ccggcgcgtc gatgaacgcg atcatcgaac cgcttcccgt
      181 ttccgtcggc ccgtcgctta gcgcgtagcc tttcttcttc aacccttccg caaacgcatt
      241 caggtcctcc acctcgaacg ccaggtgcat cagatccggc tgcaccttca cgctctcgct
      301 gttcggtaga taacacagct cgatctcctc atcgctgttc ggcgtctcca gaaacaccag
      361 ttgcgctcca cgcggcgacg tgtgccgccg tgccactttc aagccgagag cgttttcata
      421 aaatttcacc gtccgctcga gatcgttcac gcgcatccgc gtatggagaa gtttcttgat
      481 catgcgaacg cgactgtgcg cgctccacgc gccgccgcaa gtcgcacctc acatcgatcc
      541 tccatcgcca cgactctccc cttgcatacc caaccgacgc tcgcccgctt gatctcttgt
      601 cgtggcgcgc gtgtcgcgcc acatcgcgct cgcgacaccc tccttccgca accccgcggt
      661 attcgcaaga cctctgcacg gccccgcgct tgcagcgcgc ttcgccgacc gtagcgtcac
      721 ctcgatgcct gtctcgccgc cgtcgtcccg tgcttcgctc gccgaaggcc cggccacccg
      781 caccccggtg atcgaccgcc tcgctcgctg gcatcccttg ctgctcttgc tcggctgctg
      841 catcgccgcc acggcgctct gcacttggtt ctattcacgc gaagcaaaac acgccgaagc
      901 cgtgttgcgg gaacgcgaga cggttcgcgt cggcgccttc ggacatcaat tgaccgagaa
      961 cttcgcctcc aaggccgcgc gtctgcgcac gctcgcggcg agccagaccc tgggcgaatt
     1021 tctcgaacac ggtggacgcg aatacctcga tcgtttcgcc cgcgacgcgt gggtgctgca
     1081 tctccaccac gacgacatcg atcagatcag ctacatcgat aacaacggca gggagcagat
     1141 gcgcgtcgac aacgacgagg gcatcctgcc ggccaccgcg atgcgcgatc gcgcaaacct
     1201 tccgctgttt caagaggcgc ttcatctcca gcccggcgag gtccgccgct cccgcatcga
     1261 tctcgacgag gaaaacggca tcgtgacaac cccgtggaaa ccgcgcctcc gcttcgctgc
     1321 ggccgtcttc gacgaccaag gcgagcggcg cggcgtcatc atcatcaact ttctcgcgca
     1381 acagctcgtc gagcacttcg ccaattttta cccggtctac agtcaccgcc tgcgattgct
     1441 caacgacaaa ggttactggc tgcgcaccgc caaccccgcc gatgaatggg gctttgtttt
     1501 tgccgaccgc aacgaccgca cgctcgcgcg acaagccccc gcgctctggc ggcgcgtttc
     1561 caccgaagcg cagggccaag cccgctataa cggcggactc cttacctggc gccgcttcga
     1621 tcccgcatcg accgcggcgg acacgcgccc cggcggcgcc tcgtttcttc tcgtcggctc
     1681 cgaggtctcc gccgacgagt ggaccgccac gttcgcgcag ctccggcaga cgtttaccct
     1741 cgtcggtgcc gcgctgctga tcgtcacgct cgccatcacc tggctctatc aacgtcaacg
     1801 ccgcgaagcc gaacgccgcc gcgagaccga cgagctcaac gccgccatcc ttcacagcgc
     1861 gggtgccggt gtcatcaccc tcgacgccag cggcaccgtg cgctcgctca acgtcaccgc
     1921 ccaacgcctg ctcggctggg acgccgccga aatcgtcggc cgcaaaaacg cgtccgtgct
     1981 ctacgaccgc gacgccatga aagcctttgc ccgcgagacg ggcgcagcga tgaaccgcca
     2041 ctttcgccac ggcctcgaag cactcgccgc caaggtcctc gccgccgacg gaccgatcga
     2101 acgcgaatgg atctg
//
LOCUS       DASPLT010001224         1724 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_95741, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001224 DASPLT010000000
VERSION     DASPLT010001224.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1724)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1724)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1724
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_95741"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..575
                     /locus_tag="VEA63_12525"
     CDS             <1..575
                     /locus_tag="VEA63_12525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HYD84878.1"
                     /translation="HRIGQRPLAQFGNEHNFVWKRGDSFYHGKGATPAWKDAEGRPLL
                     GLIPLNMAREILIVLGGDNEEFLSFAPHGAGRNLSRKAMLAPYRDADGEIDPSRVKEV
                     LAETTGGLDIRWYNGAPDLSESPLGYKDATKVKAQIEGFGLARVVGEIHPLGCIMAGE
                     APEPMWMKKKREKRAASKAARREWVAENEE"
     gene            762..>1724
                     /locus_tag="VEA63_12530"
     CDS             762..>1724
                     /locus_tag="VEA63_12530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD84879.1"
                     /translation="MKLMPVLLGVSIAANAALLVAVVGRPAAVDDGTEVSARRGGDAA
                     QSLGGDAGSFGNVGDRVSARAGAETKNARIRVGSWRILSERELKDVVAELRAAGYPAA
                     AIRGIIQARVKAQFDARAKELMAGVEDPPYWKRNRFGYDPKVLAAQRELQQEHQRLMR
                     ELLGPDAAEENEFSRYHRQRQFGEISAEKADDARRIMADYGDLRSEIYNRGGGMLLPE
                     DREQIDFLEKSMREDLADVLTPAERENYELRSSSTAGTLRAGLTFFEPSESEFRALYQ
                     AAKTVEEKFGSPGPSSWEKTQQRQAALNELAKQVLPPERYAEFEQ"
BASE COUNT          330 a          414 c          673 g          307 t
ORIGIN      
        1 tgcaccggat cggtcagcgt ccgctcgcgc agtttggcaa cgagcacaat ttcgtgtgga
       61 agcgcggaga ctcgttttat catggaaaag gcgcgacgcc ggcgtggaag gatgcggaag
      121 ggcggccgct gctcgggctg attccgctga acatggcgcg cgaaattttg atcgtgctcg
      181 gcggcgacaa cgaggagttt ctgtcgtttg cgccgcatgg cgcggggcga aatttatcgc
      241 gcaaggcgat gctcgcgccg tatcgcgatg cggacggcga gatcgatccg tcgcgggtga
      301 aggaagtgct cgcggagacg acgggcgggc tggatatccg ctggtataac ggcgcgccgg
      361 atttgtcgga gtcgccgctc ggctacaagg acgcgacaaa agtgaaggcg cagatcgaag
      421 gtttcgggct cgcgcgcgtg gtgggggaaa ttcatccgct cggctgcatc atggcgggcg
      481 aggcgccgga gccgatgtgg atgaaaaaga agcgggagaa acgcgcggcg agcaaagcgg
      541 cgcggcggga atgggtggcg gagaatgagg agtgagggcg gccggggaat tttcgatttt
      601 ggattttcga atttcgattg attggagcat gcgcggcggg aggagagaac acggtcaggt
      661 tgaccgtgct acggcacggg cggggtggtg gaaaaagagt gcgcggcagt tctaagattg
      721 ctgcgggtgg agcgttgggt gcgtattact tgcctcaact tatgaaactg atgccggtgt
      781 tgctgggggt ttcgatcgcg gcgaatgccg cattgctcgt ggcggtggtg ggtcgccctg
      841 ctgctgttga cgacggaact gaagtgtcgg cgcgccgtgg cggcgatgcg gctcaatcgc
      901 tgggaggcga cgcggggtcg ttcgggaatg tgggggatcg cgtgagcgcg cgtgcaggtg
      961 cggagacgaa gaacgcgcgg atacgggtgg gttcgtggcg gattttgagc gagcgcgagt
     1021 tgaaggacgt cgtcgcggag ctgcgtgccg ccggttatcc cgccgccgcg attcgcggca
     1081 tcattcaggc gcgggtgaag gcgcagttcg acgcgcgagc gaaggagttg atggccggcg
     1141 tggaggaccc gccgtattgg aagcgaaacc gttttggcta cgacccgaag gtgctcgcgg
     1201 cgcagcgcga gttgcagcag gagcaccagc gcttgatgcg tgaactgctc gggccggatg
     1261 cggcagagga gaacgaattc agccgctatc atcgccagcg gcagtttggc gaaatttccg
     1321 ccgagaaagc ggatgatgcg cgaaggatca tggcggacta cggagatctg cggagcgaaa
     1381 tctacaaccg cggcggcggg atgcttttgc cggaggatcg cgagcagatc gactttctgg
     1441 aaaagtcgat gcgcgaggat ctggcggatg tgcttacgcc ggcggagcgc gagaactacg
     1501 aacttcgctc gagcagcaca gcggggacgc tacgggcggg cttgacgttt ttcgagccgt
     1561 cggagtcgga gtttcgcgcg ctgtatcagg cggcgaaaac cgtggaggag aaattcggtt
     1621 cgccggggcc gtcgtcgtgg gaaaaaacgc agcagcggca ggcggcgctc aacgaactcg
     1681 cgaagcaggt gctgccaccg gagcgttatg cggagttcga gcag
//
LOCUS       DASPLT010001225         1990 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_301961, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001225 DASPLT010000000
VERSION     DASPLT010001225.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1990)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1990)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1990
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_301961"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..358
                     /locus_tag="VEA63_12535"
     CDS             <1..358
                     /locus_tag="VEA63_12535"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HYD84880.1"
                     /translation="ASMLVGLIVGAVIVYQILYTDVTEHLPEYATLKAMGYADGFFTR
                     LVLGQSLVLTVAGYVPGVIVSFVLYRAMGAATNLAPRITWVNLAVVFVLTAAMCAAAG
                     ALATRRLRLARPAEIF"
     gene            362..1618
                     /locus_tag="VEA63_12540"
     CDS             362..1618
                     /locus_tag="VEA63_12540"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HYD84881.1"
                     /translation="MSAAPEPIITVRGLRHVYGEGSLAREVLHGIDVEFRAGEIAIIM
                     GHSGSGKTTFLTLVSALREVQTGEIVVAGNALRGATAARLTEARRAIGFVFQNHNLLG
                     SLTARQNVMMALLLDGTANDATAAHADALLARVGLAGHEHKRPREMSGGQQQRVALAR
                     ALARRPRVLMADEPTASLDAQTGREMIELIQGLARSEGCAVLIVTHDSRILDVADRIL
                     TLDDGHLVESDAMFERLLDEMAALHDLLGTVVEDPAATPGVSRRFERTAEALATRVAG
                     YVGLRLRSDLAQRADALQQLLGTLRAFEVALVDFSATAAAAPRDLREGLGDVLTQSLG
                     AVMLTAGDALRVRTEENGRLLRELTGDRSDLLKRVRDLQLARTPDADDERRTAVFALT
                     NRFARAIYLLGQVPRFLEEFRRRGAA"
     gene            complement(1631..>1990)
                     /locus_tag="VEA63_12545"
     CDS             complement(1631..>1990)
                     /locus_tag="VEA63_12545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD84882.1"
                     /translation="DKWFAGVSVSLTQTDLDVADGRIRTLNVAPRVGRPFSWGAVWVG
                     ANFLDSTEKSSGVFQLPPGFPLPVSSVAYSAEFGPSSRWNAALGVRYDLHRHLVLTAE
                     AGFIQRTSFRLAAEVVW"
BASE COUNT          319 a          533 c          789 g          349 t
ORIGIN      
        1 cgcttcgatg ctcgtcggtc tcatcgtggg ggcggtgatc gtgtatcaga ttctttatac
       61 ggacgtgacg gagcatttgc ccgagtatgc gacgttgaag gcgatgggtt acgcggacgg
      121 atttttcacg cgactggtgc tggggcagtc gctggtgctg acggttgcgg gctatgtgcc
      181 gggcgtgatc gtttcgttcg tgctgtatcg cgcgatgggc gcggcgacga atctggcgcc
      241 gcgaatcacg tgggtgaatc tagcggtggt gttcgtgctt acggcggcga tgtgtgcggc
      301 ggcgggagcg ctggccacgc ggcggctgcg gctggcgcgg ccggcggaga ttttctgagc
      361 gatgagcgcg gcgcctgaac ctatcatcac cgtgcgcgga ctgcgtcatg tttatggcga
      421 aggctcgctt gcgcgcgaag tgttgcacgg gatcgatgtg gagtttcgcg cgggtgagat
      481 cgcgatcatc atggggcata gcggctcggg gaaaacgaca tttctgacgc tggtgagtgc
      541 gctgcgcgag gtgcagacgg gcgagatcgt ggtcgcggga aacgcgttgc gaggggcgac
      601 ggcggcgcgg ttgaccgagg cgcgacgggc gatcggtttc gtttttcaaa atcacaacct
      661 gctcggatcg ctgaccgcgc ggcagaacgt gatgatggct ctgttgctcg acggcacggc
      721 gaacgacgct acggccgcgc acgccgacgc gctgctcgcg cgggtggggt tggcgggaca
      781 cgaacacaag cgtccgcgcg agatgtcggg cgggcagcag caacgcgtcg cgctcgcgcg
      841 cgcactggcg cggcggccgc gggtgttgat ggcggacgag ccgacggcgt cgctcgatgc
      901 gcaaacgggg cgcgagatga tcgagctgat tcaggggctc gcgcgaagcg aaggctgcgc
      961 ggtgttgatc gtgacgcacg actcgcggat tctcgacgtg gcggaccgca ttctgacgct
     1021 ggacgacgga catctagtgg agagcgacgc gatgttcgag cggttgttgg acgagatggc
     1081 ggcgttgcac gacctgctcg ggacggtggt ggaggacccg gcggcgacgc cgggcgtttc
     1141 gcggcggttc gagcggacgg cggaagcgct tgcgacgcgg gtggcgggtt acgtggggct
     1201 gcggctgcga tcggatctcg cgcagcgcgc ggatgcgttg cagcaacttc tcggcacgct
     1261 gcgggcgttt gaggtggcgc tggtggattt ttccgcgacg gcggcggcgg caccgcgcga
     1321 tttacgcgaa ggcttgggcg atgtgctcac gcagtcgctc ggcgcggtga tgttgacggc
     1381 cggcgatgcg ctgcgcgtgc ggaccgagga gaacgggcga ttgctgcgcg agcttacggg
     1441 cgatcgcagc gatttgttga agcgggtgcg cgacctgcaa ctcgcgcgca cgcccgatgc
     1501 ggacgacgaa cggcggacgg cggtgtttgc gctgacgaac cggtttgcgc gcgcgatcta
     1561 tctgctcggt caggtgccgc gtttcctcga ggagttcagg cggcggggag cggcgtgaaa
     1621 gcgcggcaga ttaccagacg acttcggcgg cgagccggaa gctggtgcgc tgaatgaagc
     1681 cggcttcggc ggtgagcacg aggtgacggt gaaggtcgta gcgcacgccg agagcggcgt
     1741 tccagcgcga ggacgggccg aactcggcgg agtaggcgac gctgctgacc ggaagcggaa
     1801 agcccggcgg cagttgaaac acgccgctgg atttttcggt cgaatcgaga aaattggcgc
     1861 cgacccagac cgcgccccag gaaaacggac gaccgacgcg cggcgcgacg ttgagggtgc
     1921 gaatacggcc gtcggcgacg tcgagatcgg tctgtgtcag agaaacggat acaccggcga
     1981 accatttatc
//
LOCUS       DASPLT010001226         2735 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_179387, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001226 DASPLT010000000
VERSION     DASPLT010001226.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 2735)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2735)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2735
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_179387"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1386
                     /locus_tag="VEA63_12550"
     CDS             <1..1386
                     /locus_tag="VEA63_12550"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HYD84883.1"
                     /translation="SGDMLLAAARRGFEVTGVEYSEHACATARQRLAREGLSGEVVCG
                     ELETLGHRSAEFDVCIMADVIEHVRDPRRFIQLVHQLLRPGGVVFIATPSLDSWSAKL
                     MATRWMEFKPEHLSYFSRKTLESLLFAEGFRTLVWQPGHKILNYDYVQEHFVKFPVPF
                     FTSVVKKLGRCLPSGVRSREHRIVASGMVMMGRRDEKPARPRLSIIMPVFNEAGTFRD
                     AFIRLLAHPFPAFDVEYLVVESNSTDGTRAIVNEFAATPAVRIILEDRPRGKGHAVRT
                     GLAAATGDFIAIQDADLEYDLDDYDALLEPLLNNREAFVLGARHGGRAWKMRQFTGQP
                     IASLLMNFGHLFFTTLLNVCFLVRLRDPFTMYKLFRRDCIHGLPFECNRFDFDWELVI
                     KLIRRGYIPIEIPVNYRSRSFKEGKKVSVIRDPLTWLRAVVRFRFSRLPRWAEPVPQS
                     TAQPAETSLAR"
     gene            1411..2400
                     /locus_tag="VEA63_12555"
     CDS             1411..2400
                     /locus_tag="VEA63_12555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HYD84884.1"
                     /translation="MQRAFVTGAAGFIGSNLVERLLADNVAVTGWDNLSTGQEKFLTA
                     AGAHPNFTLVRGDNLDLPALTRAMTGCDTVFHLAANADIKDGWKHPRRDLEQNTIATF
                     NVLEAMRANGVRRIAFSSTGSTYGEALVTPDRPTPETDAFPIQTSLYAASKVAGEGLI
                     SSYAEGGQLDEAYIFRFVSILGERYTHGHVFDFYQQLLQHPDRLRVLGDGRQRKSYLY
                     VQDCIDAITHVIRLGTARDAKHHTQIYNLGTPEYVEVNDSIAVICDALGLNPKFEYTG
                     GDRGWIGDNPFIFLDTKKIQSTGWKARFTIRESIVKTLRWLEANRWVFDARAS"
     gene            2418..>2735
                     /locus_tag="VEA63_12560"
     CDS             2418..>2735
                     /locus_tag="VEA63_12560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD84885.1"
                     /translation="MPEPVCRICQAATAPAGASRGRRTGREFALRRCTDCGFVSVVDP
                     WTDYAQIYDERYYQGRGSDPLVDYAYEFAQPDRTIRGYEWRGLARTINHLHPAPGKWL
                     DYGC"
BASE COUNT          490 a         1015 c          702 g          528 t
ORIGIN      
        1 agcggcgaca tgctgctcgc cgccgcccgc cgcgggtttg aggtgacggg cgtcgaatac
       61 tccgagcatg cctgcgccac ggcgcgtcag cggctcgcgc gcgaaggtct gagcggcgaa
      121 gtcgtttgcg gcgaactcga gacgctcggt caccgcagcg ccgagttcga tgtttgcatc
      181 atggccgacg tcattgagca cgtccgcgat ccccgccgct tcatccaact cgtccaccag
      241 ttgcttcgcc ccggcggcgt cgtgttcatc gccaccccat cacttgattc ttggtcggcg
      301 aaattgatgg cgacgcgttg gatggaattc aaacccgagc acctctccta cttcagtcgc
      361 aaaacactcg agagtctcct tttcgccgag ggcttccgca ctcttgtttg gcaaccgggc
      421 cacaagattc tcaactacga ctacgttcag gaacacttcg tcaagttccc cgtccccttc
      481 ttcaccagcg tcgtgaaaaa gctcggtcgt tgtctgcctt ccggggtgcg ctcccgcgaa
      541 catcgcatcg tcgccagcgg catggtcatg atgggccggc gtgacgagaa acccgcccgc
      601 ccccggctct cgatcatcat gcccgtcttc aatgaggcgg gcacgtttcg cgacgcgttc
      661 atccgcctgc tcgcccaccc tttccccgct ttcgatgtcg aatacctcgt ggtcgaaagc
      721 aactccaccg acggcacgcg cgccatcgtc aatgaattcg ccgccacgcc cgccgtgcga
      781 atcatcctcg aagatcgccc tcgcggaaaa ggccacgccg tccgcaccgg ccttgccgcc
      841 gccaccggtg acttcatcgc gattcaggac gccgatctcg aatacgacct cgacgattac
      901 gacgccctgc tcgaaccgct gctgaacaac cgcgaggcct tcgtcctcgg cgcgcgccac
      961 ggcggacgtg cctggaaaat gcgccagttc accggccagc ccatcgcctc gctgctgatg
     1021 aacttcggcc acttattttt cacgacgctg ctcaacgtct gctttctcgt tcggctccgc
     1081 gatccgttca cgatgtataa gctcttccgc cgcgattgca ttcacggcct cccattcgag
     1141 tgcaaccgct tcgatttcga ttgggaactt gtcatcaagc tcatccgccg cggctacatt
     1201 ccgatcgaga ttccggtcaa ctaccgttcc cgctccttca aggagggcaa aaaggtttcc
     1261 gtcattcgcg atccgctcac ttggctgcgc gccgtcgtcc gtttccgctt ctcccgcctc
     1321 ccccgctggg ccgaaccggt cccgcaatcc actgcgcagc ccgccgaaac ctccctcgcc
     1381 cgttaagcgc ggcactccct tcatcgtttc atgcaacgcg cattcgtcac cggcgccgcc
     1441 gggttcatcg gttccaatct cgtcgaacgt ctcctcgcgg acaacgtcgc cgtcaccggc
     1501 tgggataacc tttccaccgg tcaggaaaaa ttcctcaccg ccgccggtgc gcacccaaac
     1561 ttcaccctgg tccgcggcga caatctcgac ttgcccgcgc tcacccgcgc gatgaccggc
     1621 tgcgacacgg tttttcatct cgccgccaac gctgacatca aagacggctg gaagcacccg
     1681 cgtcgcgatc tcgagcaaaa caccatcgcc acgttcaacg tcctcgaagc catgcgcgcc
     1741 aacggcgttc gccgcatcgc gttctcctcg accggctcca cctacggtga agcgctcgtc
     1801 acgcccgatc gccccacgcc cgaaaccgac gcgtttccga tccaaacttc tctctacgcc
     1861 gcctcgaagg tcgccggcga gggcttgatc tcctcctacg ccgaaggcgg ccagctcgac
     1921 gaggcctaca tcttccgctt cgtctccatc ctcggcgaac gctacacgca cgggcacgtc
     1981 ttcgatttct accaacaact cctgcaacat ccggatcgcc tccgcgtgct cggcgacggt
     2041 cgccagcgca aaagctatct ctacgtgcag gactgcatcg acgcgatcac gcatgtgatc
     2101 cgactcggca ccgctcgcga tgcgaagcat cacacccaaa tctacaacct cggcacgcct
     2161 gaatacgtcg aggtcaacga ctccatcgcc gtcatctgcg acgcgctcgg tctgaatccg
     2221 aaattcgaat acaccggcgg cgaccgcggc tggatcggcg acaatccgtt catcttcctc
     2281 gacacgaaaa aaatccagtc caccggctgg aaagccaggt tcaccatccg ggaaagcatc
     2341 gtgaaaaccc tccgctggct cgaagccaat cgctgggtgt tcgacgcccg cgcgagctga
     2401 cgcgcccttt agccgcgatg cccgaacccg tttgccgcat ttgccaggcc gcgaccgctc
     2461 ccgccggcgc gagtcgcggc cgccgcaccg gccgtgagtt cgcgctccgc cgctgcaccg
     2521 attgcggttt cgtttccgtc gtcgatccgt ggaccgatta cgcccagatc tacgacgaac
     2581 ggtattacca gggccgcggt tccgacccgc ttgtcgacta cgcttacgaa ttcgcccagc
     2641 ccgaccgcac catccgcggc tacgaatggc gcggactcgc gcgcacgatc aatcacctgc
     2701 atcccgcccc gggaaaatgg ctcgactacg gctgc
//
LOCUS       DASPLT010001227         1238 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_179392, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001227 DASPLT010000000
VERSION     DASPLT010001227.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1238)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1238)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1238
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_179392"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..261
                     /locus_tag="VEA63_12565"
     CDS             <1..261
                     /locus_tag="VEA63_12565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007361644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HYD84886.1"
                     /translation="EIEPVMEFDNIETVKRAVEIDHGVAIVPQATVLQEVQQGTLAAV
                     RFKGKDFTRPLAILHRKGRVLTPAMKKFVEILGLEMSDTAVA"
     gene            363..860
                     /locus_tag="VEA63_12570"
     CDS             363..860
                     /locus_tag="VEA63_12570"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HYD84887.1"
                     /translation="MKILRFALTSWVLTAMAILSQAEVVAPVAAPAWELKDVNGNVVR
                     SDQFKGKVVVVDFWATWCGPCRMEIPGYVELQKKHGDKLAIIGMSVDQAGPGVVKKFM
                     EKNKVDYTVVMADEDVVSKFGGSEGINAIPTTFIIDREGNIRDRKVGAEHTEDFGKRL
                     AKYLE"
     gene            complement(996..>1238)
                     /locus_tag="VEA63_12575"
     CDS             complement(996..>1238)
                     /locus_tag="VEA63_12575"
                     /inference="COORDINATES: protein motif:HMM:NF015291.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH3 auxin-responsive promoter family protein"
                     /protein_id="HYD84888.1"
                     /translation="TGPLLASEIDTQLMARSRRYEERRREGALESPFVRLVMPGFFEH
                     WLRHHGKLGGQHKIPASRHDRFIADELSAMACFNAD"
BASE COUNT          245 a          342 c          419 g          232 t
ORIGIN      
        1 gagatcgagc ccgtgatgga gttcgacaac atcgaaaccg tgaagcgcgc ggtcgagatc
       61 gaccatggcg tcgcgattgt gccgcaagcg acagtgctgc aggaagtgca gcaaggcacg
      121 ctcgccgccg tccgtttcaa ggggaaggac ttcacgcgcc cgctcgcgat tcttcatcgg
      181 aaaggccgtg tgctcacgcc ggcgatgaag aagttcgtgg agattctcgg tctcgagatg
      241 tcggatacgg ccgttgcgtg agggtgtgaa agccggcccg gcgaggtgct ggcggcccgc
      301 gtttcccgcg cgggaataaa aggggtttga gactcctctg atttcacgca cgtttcgttc
      361 gcatgaaaat cctacgtttc gctctcactt cctgggtgtt gaccgccatg gcgatcctgt
      421 cgcaggcgga agtcgtcgcg ccggttgcag ctcccgcgtg ggagctgaag gacgtcaacg
      481 gcaacgtcgt tcgctccgac caatttaaag gcaaggtcgt ggtcgtcgac ttctgggcga
      541 cgtggtgcgg cccgtgccgg atggaaattc cgggttacgt cgaactgcag aagaagcatg
      601 gcgacaagct cgccatcatc ggcatgtcgg tggatcaagc ggggccgggc gtggtgaaaa
      661 agttcatgga gaagaacaag gtcgactaca ccgtcgtcat ggccgacgag gacgtggtgt
      721 cgaagttcgg cggttccgaa ggaattaacg ccattccgac cacgttcatc atcgatcggg
      781 aaggcaacat ccgtgatcgc aaggttggcg cggagcacac cgaagatttc ggaaagcgac
      841 tggcgaagta tctggaataa tcgtgggccg cggcggattc ccgctggagg cttcgagaaa
      901 agtgaggcgg gtcttcgccc cgcctcttcg ggcggttaaa gctcgctgcg aaacgtagac
      961 tcgcgagcgg gccggaccga taggacgggc agcggtcagt ccgcgttgaa gcaggccatc
     1021 gcgctaagtt cgtccgcgat gaaccgatcg tggcggctgg cgggaatttt atgctggccg
     1081 ccgagtttcc cgtgatgacg cagccagtgc tcgaaaaagc cgggcatcac caggcggacg
     1141 aaaggcgatt cgagcgcgcc ttcgcgccgc cgttcctcat agcggcggct ccgggccatg
     1201 agctgggtgt cgatctcact cgcgagcagc ggccccgt
//
LOCUS       DASPLT010001228         1498 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_116128, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001228 DASPLT010000000
VERSION     DASPLT010001228.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1498)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1498)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1498
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_116128"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..304
                     /locus_tag="VEA63_12580"
     CDS             <1..304
                     /locus_tag="VEA63_12580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD84889.1"
                     /translation="SSVGVTGALADPQIELFDANNARIPLGDEWAVPAATQATGANPL
                     LPDSRDAAYVVWLNPGVYSAHIFSASGGSGDVVAEVYDIPEDTWLINPAATQPPTP"
     gene            complement(730..>1498)
                     /locus_tag="VEA63_12585"
     CDS             complement(730..>1498)
                     /locus_tag="VEA63_12585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009509310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HYD84890.1"
                     /translation="ALKEIFGDQAFRLKHRLSAVNSINLARVLAQCVYYLHAWLRMAD
                     DDRDHVEFVVPTGNFGNVLAGWLLQQMGVPIRGFKVATNQNDILHRLFTTGEYRVSNV
                     QPSLAPSMDIQVASNFERFLYYSVGRDASRVREVMQAFKTTGEYRFENFDRATFSASR
                     CTDAEIPEIIQRVYRDYGYVVDPHTACAFKQHDPERVNIVLSTASPAKFPNTIQAAIG
                     IEPTHPSLEALKSRPIVKHHVKAEPAAIRAFIEAHAV"
BASE COUNT          282 a          533 c          407 g          276 t
ORIGIN      
        1 ttccagcgtc ggcgtgaccg gcgcgcttgc cgatccgcag atcgagcttt tcgacgccaa
       61 caacgcgcgc attccgctcg gcgacgaatg ggccgtgccc gccgccacgc aagcgaccgg
      121 cgcaaatccg ctgctccccg acagccgcga cgcggcctat gtggtctggc tcaatcccgg
      181 cgtttactcc gcgcacatct tcagcgcgag cggcggcagc ggcgacgtcg tcgcggaagt
      241 ttacgacatt cccgaggaca cgtggctcat caaccccgcc gccacccaac cgccgacgcc
      301 ctgagacgcc ctgcaccccg agccaccgtc gcgcagtgga aaccgtctgg tcgccgagaa
      361 acatgcacgc ccaaaggtgg agcccgacgt cctcgtcggg cttgatgacc ttcgctcgca
      421 acagcccgat gggacgtcgg gctccacctt ctactgcatg agttcggctg agccggaatc
      481 atgcagacca agaatgacga atgggttcga atgaacacgt ataccccgca taggcacagc
      541 gcctttggcg ataatcccgt cactcttcca tgttacactg aacaaccgcc ttcaggcggg
      601 catttattcg tgtctcttcg tgtccattcg tggttaaact gaattgttca aagcccgcgc
      661 gaaagcgcgg gcttttgctg cgctccatcc gcccacctca ccgccgcgtc ctccgcggcg
      721 cgcacgcctt tacaccgcgt gcgcctcgat aaacgcccgg atcgctgccg gctccgcctt
      781 cacgtgatgc ttcacgatcg gccggctctt caacgcctcc aggctcggat gcgtcggctc
      841 gatcccgatc gccgcctgaa tcgtgttggg aaatttcgcc ggactcgccg tcgacaagac
      901 gatgttcacg cgctccggat catgctgctt gaacgcgcac gccgtgtgcg gatcgacgac
      961 gtagccgtaa tcccgataca cgcgctggat gatctccggt atctccgcgt ccgtgcaccg
     1021 cgacgccgaa aatgtcgctc ggtcgaaatt ctcgaaccga tactcgcccg tcgtcttgaa
     1081 cgcctgcatc acctcgcgca cgcgcgacgc atcgcgaccc acgctgtaat agagaaaccg
     1141 ttcgaaattc gacgccactt gaatgtccat cgacggcgcc aggctcggct gcacgttgct
     1201 cacccgatac tcgcccgtcg tgaacagccg atggagaatg tcgttctgat tggtcgccac
     1261 cttgaagccg cggatgggca cgcccatctg ctgcagcagc cagcccgcga gcacatttcc
     1321 aaagttcccc gtcggcacga cgaactccac gtggtcccga tcgtcgtccg ccatgcgcaa
     1381 ccacgcgtgc aggtagtaaa cgcactgcgc gagcacgcgc gccaggttga tcgagttcac
     1441 cgccgacaac cggtgtttca gccgaaacgc ctggtcgccg aaaatctcct tcaacgcc
//
LOCUS       DASPLT010001229         1210 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_179429, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001229 DASPLT010000000
VERSION     DASPLT010001229.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1210)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1210)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1210
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_179429"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..823
                     /locus_tag="VEA63_12590"
     CDS             <1..823
                     /locus_tag="VEA63_12590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD84891.1"
                     /translation="ELRAEVENDLPDADDFIVWSDGSYADTDHDTFSELPVTRIPDAK
                     SRDLFIAALTADAAPSAPRKLSVRNAQRPFATAVFRQIAGRGSELVSIPATTRESRAV
                     QADMQGNVYLMLHGSDGDGSALWGEDAASGRLVESIDLTCLPTRASGVIFTGACWGAL
                     TVTRKAATQPLDRPPQSKSPDQSLALAALRSGYLAFIGCTGSHYSPLGRKADSAGGPL
                     HTLFWKYLTAGKPPAEALFAAKSDYAAKIPYRDDPIEEAVDQKIVRQFTCLGLGW"
     gene            883..1146
                     /locus_tag="VEA63_12595"
     CDS             883..1146
                     /locus_tag="VEA63_12595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD84892.1"
                     /translation="MNKVVVSGWKTAQAKPKRQQSHGSDLVGASVRVLKAKSPESAPA
                     KPAAKPPAKKKLRAVYLRPAKGADSDAKLFLVDEKNRPVIAQG"
BASE COUNT          239 a          469 c          299 g          203 t
ORIGIN      
        1 cgaactccgc gccgaagtcg aaaacgatct gcctgacgcc gacgacttca tcgtttggag
       61 cgacggctct tacgctgaca ccgatcacga cacgttttcc gaactccccg tcacgcgtat
      121 ccccgacgcg aaatcgcgcg acctcttcat cgccgcgctc accgccgatg ccgcgccgtc
      181 cgcaccacgc aaactttccg tgcgcaacgc ccaacgcccc ttcgccaccg ccgttttccg
      241 ccaaatcgcc ggccgcggta gcgaactcgt ttcgattccc gcgaccaccc gcgaatcccg
      301 cgccgtgcaa gccgacatgc agggcaacgt ttacctgatg ctccacggct ccgatggcga
      361 cggttccgcg ctctggggcg aagacgccgc ctccggccgc ctcgtcgaat ccatcgacct
      421 cacgtgtctt ccgacccgcg ccagtggcgt gattttcacc ggtgcttgct ggggcgcgct
      481 caccgtcacc cgcaaagccg ccacgcaacc gctcgatcgc ccgccgcagt cgaaaagccc
      541 cgaccaatcg ctcgcgctcg ccgcgctgcg cagcggctat ctcgccttca tcggctgcac
      601 cggttctcac tactcgccgc tcggccgcaa agccgacagc gccggcggtc cgttgcacac
      661 gctgttctgg aaatacctca ccgccggcaa accgcccgct gaagcgctgt tcgccgcgaa
      721 gtccgattac gccgcgaaaa tcccttaccg cgacgatccc atcgaagaag ccgtcgatca
      781 gaaaatcgtc cgccaattca cctgcctcgg tctcggctgg tgacacgttc tccatcccga
      841 ttgccccttg cgcaaaaacc aaatcaaaac cacgtttcac ccatgaacaa agtcgtcgtc
      901 tccggctgga aaaccgccca ggcaaagccg aagcgccagc aaagtcacgg ctccgatctc
      961 gtcggcgcat cggtccgcgt gctcaaagcg aaatcccccg agtccgcccc cgcgaaacca
     1021 gccgcaaaac cgcccgcgaa gaaaaaactc cgcgccgttt atctccgtcc ggccaaaggc
     1081 gccgactccg atgcgaagct tttcctcgtc gacgaaaaga accgccccgt catcgcgcag
     1141 ggctgagctt cgcgaaattc acttttcttc gctcggtttc aaatcgaccg agcccggctc
     1201 gggcaaacca
//
LOCUS       DASPLT010001230         1492 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_179452, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001230 DASPLT010000000
VERSION     DASPLT010001230.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1492)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1492)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1492
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_179452"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..752
                     /gene="fliP"
                     /locus_tag="VEA63_12600"
     CDS             <1..752
                     /gene="fliP"
                     /locus_tag="VEA63_12600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009507957.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HYD84893.1"
                     /translation="PPADTANDAPAANAPTADSGDTVNADPFRLNIGLEGTGQSGQVS
                     VAVQIVLVMTLLSVAPSIVLLMTTFTRLVIVLGFVRTALGTPTAPSNQIVVGLSLFLT
                     FFLMGPVFDRIHAEALNPYFDKEITASEALDRATIPLKEFMLKQTRTRDLEYFLELGG
                     FGPTAVKDLPMRVVIPAFVISELQTAFQMGFLLFLPFLVIDFLVSSVLMALGMMMMPP
                     VTVALPLKLLLFVLVDGWHLVVQSLVQSFVV"
     gene            861..1130
                     /locus_tag="VEA63_12605"
     CDS             861..1130
                     /locus_tag="VEA63_12605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliQ"
                     /protein_id="HYD84894.1"
                     /translation="MNPEIAIDLFKTTVVFALYVVAPFLILTLVVGLVTSLVQSVTSL
                     QEQTLTFVPKLLTLAAMLLILTPWLLRSLSEFTTTIIARMGSLGH"
     gene            1232..>1492
                     /locus_tag="VEA63_12610"
     CDS             1232..>1492
                     /locus_tag="VEA63_12610"
                     /inference="COORDINATES: protein motif:HMM:NF013478.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HYD84895.1"
                     /translation="MSLEFLVTWMMVFLRTTGVILQLPAVAGRPIPMLARGGICICIA
                     SLLVGVVPRADIPLTLGALALAAGGELLLGLALGFVGRMAFAA"
BASE COUNT          232 a          491 c          428 g          341 t
ORIGIN      
        1 cgccgcccgc cgacaccgcg aacgacgcgc ccgcggcgaa cgcgcccacg gccgactccg
       61 gcgacacggt caacgcggat ccctttcgcc tgaacatcgg cctcgaaggc accggccagt
      121 ccggacaggt cagcgtcgcc gtgcagatcg tgctggtgat gacgctcctt tccgtcgcgc
      181 cgtcgatcgt cctgttgatg accacgttca cgcgtctcgt gatcgtgctc ggctttgtgc
      241 gcaccgcgct cggcacgccg acggcgccgt ccaatcagat cgtcgtcggc ctctcgcttt
      301 tcctgacgtt cttcctgatg ggcccggtgt tcgaccgaat tcacgcagaa gcgctgaatc
      361 catatttcga caaagaaatc accgcatcgg aggcgctcga tcgggcgacc attccgctga
      421 aggaattcat gttgaagcaa acgcgcacgc gcgacctcga gtattttctc gagctcggcg
      481 gcttcggccc gaccgcggtg aaagacctgc cgatgcgcgt ggtgattccg gccttcgtca
      541 tcagcgaact gcagaccgca tttcaaatgg gcttcctgct gtttctgccg ttcctcgtca
      601 tcgattttct cgtgtcatcg gtgttgatgg cgctcggcat gatgatgatg ccgcccgtga
      661 cggttgcgct gcccctgaaa ctgctgctct tcgtcctcgt cgacggctgg cacctcgtcg
      721 tgcagtcgct cgtgcagagc ttcgtggtgt gacgatgcaa gctcagagct gagagcccag
      781 agctcagagc tcgaaccacc gaactccaag tgtccttcct gctctcagct ctagactctc
      841 agcgctaagc ttttcgaatc atgaatcccg aaatcgccat cgatcttttc aagaccaccg
      901 tggtcttcgc gctctacgtg gtcgcgccgt tcctcattct cacgctcgtc gtcggactgg
      961 tgacgagcct tgtccagtcg gtgacgagtt tgcaggagca gacgctcacg ttcgtgccga
     1021 aactgctcac gctcgctgcg atgctcctga ttcttacgcc gtggctgctg cgctcgttgt
     1081 cggaattcac gaccacgatt atcgcgcgca tgggctcgct cggccattga gccgctcgct
     1141 cgtttacttt cccggccaat tgtccgttca cgtctgcgcc gcgcatcgac gagaacgagc
     1201 acgattctga aaactcccga cgacgcgcgt catgtccctc gaattcctcg tcacctggat
     1261 gatggttttt ctgcggacca cgggagtgat tctgcaattg cccgccgtgg cgggacgccc
     1321 gattccgatg ctggcgcgcg gcggcatctg catttgcatc gcgagtttgc tcgtcggtgt
     1381 cgtgccgcgc gcggacatac cgctgacgtt gggggcactg gctcttgccg ctggcggtga
     1441 actcctgctc gggctcgcgc tgggctttgt cggtcgcatg gcattcgccg cc
//
LOCUS       DASPLT010001231         2613 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_199816, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001231 DASPLT010000000
VERSION     DASPLT010001231.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 2613)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2613)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2613
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_199816"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            2..718
                     /locus_tag="VEA63_12615"
     CDS             2..718
                     /locus_tag="VEA63_12615"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017397564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HYD84896.1"
                     /translation="MITGASSGLGEAAARRLSAEGATVVLGARRTERLAALSRELGAK
                     GGKSLVVATDVTDRAQLQKLVDSAVETFGRIDVLINNAGVMPLSPVEKLKVEEWDRMI
                     DVNIKGVLYGIAAALPYMQRQKVGHIINVSSVAGHKVGPGFAVYSATKHAVRTLTEGL
                     RMEVKPYNIRTTIISPGAVATELLNGISDPETAAANRAYVAKVAVPADSFARAVAFAI
                     SQPAELDINEILFRPTNQEF"
     gene            861..1385
                     /locus_tag="VEA63_12620"
     CDS             861..1385
                     /locus_tag="VEA63_12620"
                     /inference="COORDINATES: protein motif:HMM:NF012417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HYD84897.1"
                     /translation="MHSSSLHRRAFLKTAGAIALAVPTLLRAQSATGATSAAKAATAI
                     EITRTGSTPSAKGPADWFTGTTRIDPLFAPNASSRAGAAHVTFEPGARTAWHTHPLGQ
                     RLIVTSGLGWVQGEGGPVQEIRPGDVVWFPSGLRHWHGATPTTAMTHIAIQEEHNGKV
                     VDWMEKVTDDQYRR"
     gene            1382..1831
                     /locus_tag="VEA63_12625"
     CDS             1382..1831
                     /locus_tag="VEA63_12625"
                     /inference="COORDINATES: protein motif:HMM:NF037716.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclophilin-like fold protein"
                     /protein_id="HYD84898.1"
                     /translation="MKIAATLRWLCGLSMIAAAPIRTASYGKAESRTMTSITIRIGKK
                     TFSALLEDNPTADAFEKMLPLTLEMRDLNDNEKVIELPAKLPGETVNPKTIQTGDLMI
                     WSSRSLVLFYKTFPTPYSYFRLGRIVDTCGLAEAVGSGSVTVTFEIP"
     gene            complement(1980..>2613)
                     /locus_tag="VEA63_12630"
     CDS             complement(1980..>2613)
                     /locus_tag="VEA63_12630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HYD84899.1"
                     /translation="TYIRQVALITLLAQIGCWVPAKSCRVGLVDRIFSRVGASDDLAR
                     GNSTFMVEMNETANILNNATDRSLIILDEIGRGTSTYDGLSIAWAVVEHLHRDADRGP
                     RTLFATHYQELTQLEKHLPRLRNFSVAVKEWNDEIVFVRRVIAGAADRSYGIQVARLA
                     GLPLSVIDRAKTILAKLESDDTSISLPAPQAKPKKKITIAPADDSQLSLL"
BASE COUNT          522 a          813 c          822 g          456 t
ORIGIN      
        1 tgtgatcacc ggtgcgagta gcggtctcgg cgaagcggcc gcccgccgtc tttccgccga
       61 aggcgcgaca gtcgtcctgg gtgcccgccg cacagaacgg ctcgccgccc tcagtcgaga
      121 attaggcgcc aaaggtggaa agtcgctcgt cgttgcgacc gatgtcacag acagagctca
      181 actccaaaag ctcgtcgact ctgcggtgga gacgttcggc cggatcgacg tgctgatcaa
      241 taacgccgga gtgatgccgc tctctccagt ggagaagctc aaagtcgagg agtgggaccg
      301 catgatcgat gtgaacatca aaggcgtgct ctacggcatc gcggcggcgc ttccgtatat
      361 gcagcggcag aaagtgggac acatcatcaa cgtttcctcc gtggccggac acaaggtggg
      421 gccggggttc gcggtctatt ctgcgacgaa gcacgccgtg cgcaccctca ccgaaggcct
      481 tcggatggag gtaaagcctt acaacattcg cacgacgatc atctcgccgg gcgcggtcgc
      541 caccgaattg ttgaatggaa tctccgatcc ggaaactgcc gcagcaaatc gggcctacgt
      601 cgccaaagtc gccgtgcccg ccgattcctt cgcgcgcgcc gtggcgttcg cgatcagcca
      661 gcccgcggaa ctggacatca acgaaatcct ctttcgtccc acgaaccagg agttctgacc
      721 acgacgagca gacacccacc ggtcccctga ggggcctggg gcctggcttt ccagcaagga
      781 aacgacagcc caagcaaccg cacgcgagcg gctgcccgcg cgagggagta tcatgcgcac
      841 gatttaacct atcccaacct atgcattcct cctccctcca tcgccgcgcc ttcctgaaaa
      901 ccgccggagc catcgccctc gccgtgccga cgctgcttcg cgcccaatcc gcaactggcg
      961 ccacgagcgc ggcgaaagcg gccacggcga tcgaaatcac tcggaccggc tcgacgccct
     1021 ctgccaaagg cccggccgac tggttcaccg gcacgacgcg cattgatccg ctgttcgccc
     1081 caaacgcatc gtcccgtgct ggggccgcgc acgttacctt cgaaccgggc gctcgcaccg
     1141 cgtggcacac gcatcccctt ggtcaaaggc tgatcgtcac gtccggtctc ggctgggtgc
     1201 agggcgaagg cggacctgtg caggaaatcc gtccgggcga tgtggtctgg tttccgtcgg
     1261 gtctcaggca ctggcacggc gcgacgccca ccaccgcgat gacgcacatc gcgatccagg
     1321 aggagcacaa cggcaaagtc gtcgactgga tggagaaggt gaccgacgac caatatcgcc
     1381 gatgaaaatc gccgccaccc tccggtggct ctgcggcctg agcatgatcg ccgcggcgcc
     1441 aatacgcaca gccagctatg gcaaagccga atcaagaacg atgacctcaa ttacaattcg
     1501 gatcggaaag aaaaccttct cggcgctcct cgaggacaat ccgactgccg atgccttcga
     1561 gaagatgctt ccgctcacgc tcgagatgag ggacctgaac gataacgaaa aagtgatcga
     1621 gctgcccgcc aagctccccg gtgaaaccgt aaatccgaaa acgattcaga cgggggacct
     1681 catgatctgg agttcgcgca gcctcgtgct cttctataag acctttccca cgccctacag
     1741 ctacttcagg cttgggcgca tcgtggacac gtgcggactt gcggaggcag tcggttcggg
     1801 aagtgtcacc gtcacattcg agataccgta acctacgatc gaggtagtgg aacaattcca
     1861 aggttttgat ggcgatgccc cgggggagac gtctcggctc caccggcgct aagggacggt
     1921 aaggcgtcgc cgcccagttt cgaacggcca gtgcgatctt cgctaagcac ggggacaact
     1981 cacagcaggc tgagctgcga atcgtccgcg ggcgcgatcg tgatcttctt cttcggcttc
     2041 gcttgcggag cgggtaggga aatcgacgtg tcgtcgctct caagcttcgc gaggatggtt
     2101 tttgcgcggt cgatcacgct gagcgggagg ccggcgaggc gggcgacttg gatgccgtag
     2161 ctgcggtcgg cggcgccggc gatcacgcgg cggacgaaga cgatttcgtc gttccactcc
     2221 ttcacagcga ccgaaaaatt gcggaggcgc gggaggtgtt tttcgagctg agtgagctcc
     2281 tggtagtgcg tcgcgaagag cgtgcgcggg ccgcgatcgg cgtcgcggtg gagatgttcg
     2341 acgacggccc aggcgatcga gaggccgtcg taggtcgagg tgccgcggcc gatttcgtcg
     2401 aggatgatga gcgagcggtc ggtggcgttg ttgaggatgt tcgcggtctc gttcatctcg
     2461 accatgaacg tcgagttgcc gcgcgcgagg tcatcgctcg cgccgacgcg ggaaaaaatg
     2521 cgatcgacga ggccgacgcg gcaggatttc gcgggcaccc aacagccgat ttgcgcgagc
     2581 aacgtgatca acgcgacctg gcggatgtag gtc
//
LOCUS       DASPLT010001232         2307 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_116226, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001232 DASPLT010000000
VERSION     DASPLT010001232.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 2307)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2307)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2307
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_116226"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            124..>2307
                     /locus_tag="VEA63_12635"
     CDS             124..>2307
                     /locus_tag="VEA63_12635"
                     /inference="COORDINATES: protein motif:HMM:NF014850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HYD84900.1"
                     /translation="METIESLSRKRIDASDVPDRAALEHRLIRSLDFDWRFHLGDIVG
                     AQASAFDDRTWRTLNVPHDWAVEGEVKADAPGGRPNGFYPGGVGWYRRELEIGPELQG
                     RRVLLQFDGVHMNADVWVNDHWLGRYPNGFSTFYHDVTEWIKVGERNLIAVRVDNAHQ
                     PSARWYTGAGIYRHVWLIATDPLHIEPWGTTVTTPVVAADHAIVRIETRIHVGHYPET
                     RFRWTEPTATGYRFVTKRCRLVSQIHTDAGERVGEATTELEISDFSRQRVVQEIRLDR
                     PQLWSPEQPALYRVYSVLHDDAKPVDDHVTTFGVRKLTFDPVAGFRVNDRPTKLKGCC
                     LHQDAGALGRAVPEKVWRRRLASMKALGANAIRGHCPVAPEFLDLCDELGLFVLNDSF
                     DEWEHDWEKSYAEGPRGKIEYGYHKFFRQWHETDLRNHLRRDRNHPSIVMWNVGNEIP
                     EQYVRTPDAIHTLRRLVAICHEEDPTRKTTVAIEGALPQELNREFITLVDVGGFNYID
                     VKYPETYYAEHHARHPDRMLLGTETVYTLGNWLAVEQNPYVMGQFLWVGIDYLGEAVW
                     PKHGWDRGLVDIIGTAKPEYFFRQSLWSSTPMVHVTVGEADIHVNGISDIWNMPRVES
                     HWNWPPGATQPVLVYTNCDEAELFLNGRSLGTKRRAEFPERKLKWSVAYEPGELRAVA
                     RRGAEIAEHRLRTAGSPAALRMSLETPDLVADGRDIGIATVEVIDD"
BASE COUNT          450 a          675 c          764 g          418 t
ORIGIN      
        1 tcgcgaattc tggggcgagc gggtgtatcg cattctcgtt cgccggcgca gcgcggtgca
       61 ggaaggcatg agcacgtttc agatcgcacc cgctgtcgcc ggtcgttccc gctgattttc
      121 ctcatggaga caatcgaatc cctttctcga aaacgcattg atgcgagcga cgtccccgat
      181 cgcgctgcgc tcgaacatcg tctcattcgg agcctcgatt tcgactggcg ctttcatctc
      241 ggcgacatcg tcggcgcgca ggccagcgct ttcgacgatc ggacgtggcg aacgctgaac
      301 gtgccgcacg actgggccgt cgaaggcgag gtcaaggcgg acgcgcccgg cggacggccc
      361 aacggtttct atccgggagg cgtgggatgg tatcgccgcg aactcgagat cggtcctgag
      421 ctgcaaggcc ggcgcgtgct gctgcagttc gacggcgtgc acatgaacgc cgacgtctgg
      481 gtgaacgatc attggctggg gcgatacccg aatggattca gcacgtttta tcatgacgtc
      541 accgagtgga ttaaagtcgg cgagcgaaac ctgatcgcgg tgcgcgttga caacgcgcac
      601 cagccctcgg cgcgctggta tacgggagcg ggaatctacc gccatgtctg gctgatcgcc
      661 acagatccgc tgcacatcga gccgtggggc acaaccgtca cgacgccggt ggtcgcggcg
      721 gatcacgcca ttgtccgcat cgaaacgcgc atccatgtcg gccattatcc ggaaacgaga
      781 ttccgttgga ccgagccgac cgcgacgggc taccgtttcg tgacgaaacg atgccgcctc
      841 gtttcgcaga tccacacgga cgcgggcgag cgggtcgggg aagccacgac ggaactcgag
      901 atttccgatt ttagccgcca gcgcgtcgtg caggaaatcc ggctggaccg gccgcagctc
      961 tggagcccgg agcagccggc gctttatcgc gtgtattcag ttctgcatga cgatgcgaag
     1021 cccgtcgacg accacgtcac gaccttcggc gtgcggaagc tcaccttcga tccggtcgcg
     1081 ggttttcgag tcaacgaccg gcccacgaag ctaaagggtt gttgcctgca tcaggatgcc
     1141 ggcgcgctgg ggcgcgcggt tccggaaaaa gtgtggcggc gtcgtctcgc gtcgatgaag
     1201 gcgctcgggg cgaacgctat ccggggacac tgtccggtcg cgcccgagtt cctggatctc
     1261 tgcgatgaac tcggcctgtt cgtgctcaac gattcgttcg acgaatggga gcacgattgg
     1321 gaaaagagct acgcggaagg gccgcgcgga aagatcgagt atggttacca caaatttttc
     1381 cggcagtggc acgagaccga cctgaggaat catcttcgcc gcgaccgcaa tcatccctcg
     1441 atcgtgatgt ggaatgtcgg caacgagatc ccggaacagt atgtgcggac gcccgacgcg
     1501 atccacacgc tgcgccgcct cgtcgcgatc tgccacgagg aagatcccac gcgcaagacg
     1561 acggtcgcga tcgagggcgc gttgccgcag gagttgaatc gagagttcat caccctcgtc
     1621 gacgtgggcg ggttcaacta catcgacgtg aagtatccgg agacctacta cgctgagcac
     1681 cacgcccggc atcccgatcg gatgctgttg ggaacggaaa cggtctacac gttgggcaac
     1741 tggctcgcgg tggagcagaa cccttacgtg atgggccagt tcctgtgggt cgggatcgat
     1801 tatctgggcg aagcggtgtg gcccaagcac ggatgggatc gcggcctcgt cgacatcatc
     1861 ggcacggcga aaccggaata ttttttccgg cagagcctgt ggagttcgac gcccatggtt
     1921 catgtcacgg ttggagaagc ggacattcac gtgaacggaa tcagcgacat ctggaacatg
     1981 ccgcgggtcg agtcgcattg gaactggccg ccgggcgcaa cgcagccggt gctcgtctac
     2041 accaactgcg acgaagccga gttgttcctc aacggtcgct cgctcggcac gaaacggcgc
     2101 gcggagtttc cggagcggaa actcaaatgg tcggtggctt acgaaccggg cgagctgcga
     2161 gcggtggcgc ggcgaggcgc agaaatcgcg gagcaccggt tgcgcacggc gggaagtccc
     2221 gcggcgttgc gaatgagctt ggaaacaccg gatctggtgg ccgatggacg agacattgga
     2281 attgcgacgg tcgaggtgat cgacgac
//
LOCUS       DASPLT010001233         1689 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_260391, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001233 DASPLT010000000
VERSION     DASPLT010001233.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1689)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1689)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1689
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_260391"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(189..>1689)
                     /locus_tag="VEA63_12640"
     CDS             complement(189..>1689)
                     /locus_tag="VEA63_12640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SEC-C metal-binding domain-containing protein"
                     /protein_id="HYD84901.1"
                     /translation="GTAETEATEFNDICKLAVQVIPTNKPCIRVDKNDSIFKTRRDKF
                     AWVVKEIEAANKRGQPVLVGTVSVESSEVLSRMLKRAGIIHTVLNAKFHAQEADIVAR
                     AGQRGAVTIATNMAGRGTDIKLGEGVRELGGLYVVGTERHESRRIDRQLRGRCSRQGD
                     PGTTKFFLSLEDDLMRLFLQGNLASRLMEGSMQEGEELEHPWLNRSIESAQKKVEQQN
                     YSIRKRLLQYDDVLNQQREVIYGIRNGAIHAERPKDIIFEQIEEELVNRLESAGYGER
                     AGPNQTAVESLVGWVNSHFPISLRVEELAKGSVDDLAKLLVERIKKAYAIKESVEIPE
                     ALGSLERYVVINAIDHHWQEHLTEMEDLRKSIGLRSYGQKDPLVEYKGEAFRFFEELM
                     NNVRLQICTGLFRSASNLESFENMLALLSRTARAVGPSDLPAPAAPGVTPVSARSAVP
                     SGGGTATAEAPEQEIQLPKITVRREAPKVGRNDPCPCGSGKKFKQCHGR"
BASE COUNT          284 a          589 c          487 g          329 t
ORIGIN      
        1 ttgtccggcg ttcgtcgtcc gccgcgtttc cccgcgaagc gagcaaagcg aacagcatga
       61 acacgaacgg ccacacgcgg acaggggaag ctggggtcct gctcacgcgg cgagcttgcc
      121 gatccacgcc cgtcgaacaa gcgcgaactc accgtcaggc ccgccgggcg accggcggcg
      181 acctcaactc accggccgtg gcactgcttg aatttctttc cgctgccaca ggggcacgga
      241 tcgttgcgac cgaccttcgg ggcttcgcgt cgcaccgtga tcttcggcaa ctgaatctcc
      301 tgctccggcg cttcggccgt cgccgtgccg ccgccactcg gcaccgccga gcgagccgaa
      361 accggcgtca ccccgggtgc cgcgggcgcg ggcagatccg acggccccac cgcgcgcgcg
      421 gtgcggctca acaacgcgag catgttctcg aacgactcga gattcgacgc gctgcggaac
      481 agacccgtgc aaatctgcag gcgcacgttg ttcatcaact cctcgaagaa acgaaacgcc
      541 tcgcccttgt attccacgag cggatccttc tggccgtagc tgcgcaatcc gatgctcttg
      601 cgcaggtctt ccatctcggt gagatgctcc tgccagtgat gatcgatcgc gttgatcacc
      661 acgtagcgct ccagcgaacc gagcgcctcc ggaatctcga ccgactcctt gatcgcgtaa
      721 gctttcttga tccgctcgac caacagcttc gccaggtcgt cgacactccc cttcgccagc
      781 tcttcgacgc gcaggctgat cggaaaatgc gaattcaccc agccgaccag actctcgacc
      841 gccgtctggt tggggcccgc acgttcgcca tagccggcac tctcgagccg gttgaccaac
      901 tcctcctcga tctgctcgaa gataatgtct ttcggccgtt ccgcgtggat cgccccgttg
      961 cgaatcccgt agatcacctc gcgctgctgg ttcagcacgt cgtcgtattg gaggagccgc
     1021 ttgcgaatcg aataattctg ctgctcgacc tttttctgcg cgctctcgat cgagcggttc
     1081 aaccacggat gctcgagctc ctcgccttcc tgcatcgagc cttccatcag ccgcgaggcc
     1141 agattgccct gcaggaaaag ccgcatcaga tcgtcctcga gcgaaaggaa aaacttcgtc
     1201 gtgcccggat cgccctgacg cgagcaacgg ccgcgcaact gccggtcgat gcggcgcgac
     1261 tcgtgccgct cggtgccgac cacgtaaagg ccgccgagct cgcgcacgcc ctcgcccagt
     1321 ttgatgtcgg tgccgcgacc cgccatgttc gtcgcgatcg tcaccgcgcc gcgctgaccc
     1381 gcgcgcgcga cgatgtccgc ttcctgcgcg tggaattttg cgttgagcac cgtgtgaatg
     1441 atgcccgcgc gcttgagcat gcgtgaaagc acctcggagg attcgaccga caccgtgccg
     1501 acgagcaccg gctgtccgcg cttgttcgcc gcttcgattt ccttcacgac ccacgcgaac
     1561 ttgtcgcgac gcgttttgaa aatggagtcg ttcttgtcga cgcggatgca gggcttgttc
     1621 gtcgggatga cctgcaccgc gagtttgcag atgtcgttga actcggtcgc ttccgtttcc
     1681 gccgtgccg
//
LOCUS       DASPLT010001234         1374 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_280621, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001234 DASPLT010000000
VERSION     DASPLT010001234.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1374)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1374)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1374
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_280621"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..600
                     /locus_tag="VEA63_12645"
     CDS             <1..600
                     /locus_tag="VEA63_12645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD84902.1"
                     /translation="VVAQHRAAEPAEASPAAPSAEPEPAGKFARQTFPEIAPPMIDPV
                     AGKNYFLRSTMHFEDGEYAATNYARGAIVPINTPVQIVSMRGKKIGIKRLDTGEALQI
                     ENVDKYTLKSLAELARLLFSDVPTPIEQLPDELAASIRNGDMRKGMTKELVLMARGYP
                     PAHETSSIESDRWVYWSSRFVKHTVIFADGRLAEGRGLF"
     gene            complement(881..>1374)
                     /locus_tag="VEA63_12650"
     CDS             complement(881..>1374)
                     /locus_tag="VEA63_12650"
                     /inference="COORDINATES: protein motif:HMM:NF016693.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tryptophan 7-halogenase"
                     /protein_id="HYD84903.1"
                     /translation="GAEPTMGFHVTSDYSYFRQELAQERLVLIGDAAGFFDPIFSSGV
                     YMAMHSARTAVALVARAHAENRGLTLRERRHYTRALKRHASVFQRLIAAFYDPHSIAV
                     FMCEPVPWNIMPGMASIVAGHAKLTWPLWWRFQLFLLICRLQRHLRLAPPVSFNALAP
                     ANP"
BASE COUNT          268 a          370 c          501 g          235 t
ORIGIN      
        1 gtggtggccc agcatcgcgc ggccgaacca gcggaggcgt caccggcggc accctctgcc
       61 gagccggagc cggcggggaa attcgcgagg cagacgtttc ccgagatcgc gccgccgatg
      121 atcgatccgg tcgcggggaa gaattatttt ctgcgcagca cgatgcattt tgaggacggg
      181 gaatacgcgg cgacgaacta cgcgcgcggt gcgatcgtgc cgatcaacac gccggtgcag
      241 atcgtgtcga tgcgaggaaa gaagatcggc atcaagcggc ttgatacggg agaagcgttg
      301 cagatcgaaa acgtggacaa atacacgctg aagtcgctcg cggaactcgc gcggttgttg
      361 ttctccgacg tgccgacgcc gatcgagcaa ctgccggacg aactcgcggc gtcgattcgc
      421 aacggcgaca tgcgcaaagg catgacgaag gaattggtgc tgatggcgcg cggttatccg
      481 ccggcgcatg agacgagctc gatcgagagc gatcgctggg tttactggag cagccgattt
      541 gtgaagcaca cggtgatttt tgcggatggc cggctcgcgg aaggccgcgg gttattttag
      601 cgggcggcgt catcgggagc gcgttggagt ggccggatgt ggcgcggggt ctccgcacgt
      661 cgccgttatc gcgcatgcat cacacgcaga ggtggagtgc gcagtctgtt gtagcgaaac
      721 gcgtgagcgc ttcgtcgggg cgagagacga aaagctcacg cttttgtggc gcgacgagcg
      781 cgccacctac actgaattcc gctcggctca gcggctacgg tttttactta aaccactttg
      841 gtgcccgcgg cggggtcggg agacccccgc cctacatcga tcagggattc gcgggcgcga
      901 gagcgttgaa gctgacgggc ggcgcgaggc ggaggtggcg ttggaggcgg cagatcagaa
      961 ggaaaagttg gaagcgccac cataacggcc aggtgagctt cgcgtgtccg gcgacgatcg
     1021 aggccatgcc gggcatgata ttccacggca ccggttcgca catgaaaacg gcgatgctgt
     1081 gcggatcgta gaacgcggcg atcaagcgct ggaagacgct cgcgtggcgt ttgagcgcac
     1141 gtgtgtagtg gcggcgttcg cgcaaggtga ggccgcgatt ttccgcgtgc gcgcgagcga
     1201 cgagtgcgac ggcggtgcgc gcggagtgca ttgccatgta gacgccggag gaaaaaatcg
     1261 gatcgaagaa gccggcggcg tcgccgatga ggacgaggcg ttcctgcgcg agttcctggc
     1321 ggaaataact gtagtcgctc gtgacgtgaa agcccatcgt cggctccgcg ccgg
//
LOCUS       DASPLT010001235         1116 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_95852, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001235 DASPLT010000000
VERSION     DASPLT010001235.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1116)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1116)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1116
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_95852"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1116)
                     /locus_tag="VEA63_12655"
     CDS             complement(<1..>1116)
                     /locus_tag="VEA63_12655"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase/oxidase"
                     /protein_id="HYD84904.1"
                     /translation="IDRYDFAFGTSSRSSRLLHGGLRYLEQGRIGLVREASVEKKTIR
                     AIAPHLADPLGFVFPAYQKAGRPLWQLRVGVKLYDLLCSGRNFQPSRGFTRDETLERL
                     PSLNRDGLLGSVRYFDALTNDARLVLDTLRSAEAHGATVSNHMRFQDARRAGDAWECE
                     LEDTLAGETFTVRTRALVNATGPWAQEVSRSSVKLRLSKGIHLVIDRQRLPVTEAVVI
                     TERKRILFLIPWGERVIVGTTDTDYRDLPEKVRVEANDIAYVLRTVNESFPSLALEER
                     DIISSWAGLRPLIANPDGSPSDISRAHQIHQPEPGWWDVSGGKLTTYRLMAEQTVDQI
                     VRHLGRSHSTPKCRTAAEPLLSRDLIAIYSGTVPPPF"
BASE COUNT          207 a          318 c          385 g          206 t
ORIGIN      
        1 caaacggcgg cggcaccgtt ccgctgtaga tcgcgatcag atcgcgcgag agcagtggtt
       61 cagcggcggt gcggcatttg ggcgtggagt gactgcggcc caagtggcgg acgatctgat
      121 ccacggtttg ctcggccatg agtcgatacg tggtgagctt accgccggaa acatcccacc
      181 agccgggttc cggttggtga atctggtgag cgcgcgagat gtcggatggc gaaccgtcgg
      241 ggtttgcgat caacggacgc aaaccggccc acgagctgat gatgtcgcgt tcttcgagcg
      301 cgagcgaagg aaaggattcg ttcaccgtgc gcagcacgta ggcgatgtcg ttggcctcga
      361 cgcggacctt ttcgggcaag tcgcgataat cggtgtcggt cgtgccaacg atcacgcgtt
      421 cgccccaggg aatgagaaag aggatgcgtt tacgctcggt gatgacgacc gcctcggtga
      481 cgggcagccg ctggcgatcg atcacgaggt gaatgccttt gctgagccgg agttttacgg
      541 agctacgtga gacttcctgg gcccatgggc cggtggcgtt gactagggcg cgagtgcgga
      601 cggtaaacgt ctcgcccgcg agcgtgtcct cgagctcgca ttcccacgcg tcgccggcgc
      661 gacgagcgtc ttggaaacgc atgtgattgc tcaccgtggc gccgtgagct tcggcggagc
      721 gcaacgtgtc gaggacgagc cgcgcgtcgt tcgtaagcgc gtcgaaatag cgaacggaac
      781 cgagcagtcc gtcgcggttt agcgacggca aacgctcgag cgtttcgtcg cgcgtgaagc
      841 cgcgcgacgg ctggaagttt ctcccgctgc agagcaggtc gtagagtttt acgccgacgc
      901 gcaactgcca caatggccgg ccggctttct ggtaggcggg aaaaacgaag ccgagcggat
      961 ccgccagatg cggtgcgatc gcgcgaatcg tctttttctc gacgctcgct tcacgcacga
     1021 gaccgatgcg gccttgttcc agatagcgca gcccgccgtg cagcaagcga ctcgaacgac
     1081 tgctcgtgcc gaacgcgaaa tcgtagcggt cgatga
//
LOCUS       DASPLT010001236         1141 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_138571, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001236 DASPLT010000000
VERSION     DASPLT010001236.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1141)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1141)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1141
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_138571"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..507
                     /gene="kduI"
                     /locus_tag="VEA63_12660"
     CDS             <1..507
                     /gene="kduI"
                     /locus_tag="VEA63_12660"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012377064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008697 -
                     4-deoxy-L-threo-5-hexosulose-uronate ketol-isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0045490 - pectin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HYD84905.1"
                     /translation="GEREISFESNPAGQAQFYFISTPAHAKHPTAHATREQNIGAPIG
                     DAAKCNRRRINKYIHADGIKSCQLVMGFTEFEPGSVWNTMPTHTHSRRTEIYFYFDLG
                     NELVIHLLGEPTATRHIIVRDREVALSPSWSIHSGVGTGSYRFIWAMGGDNQTFSDMD
                     AVAMNDLR"
     gene            710..>1141
                     /locus_tag="VEA63_12665"
     CDS             710..>1141
                     /locus_tag="VEA63_12665"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HYD84906.1"
                     /translation="MPNILDKFRLDGRVAIVTGASRGLGAGMARALAEAGADIALVAR
                     GDTSALVSDIAALGRKSVTVAIDVAAAGAAEKIVSDTVAALGRADILINNAGIIRRAS
                     FVDFTEQDWREVVDVNLDAAFRLSQVFARQLIAKKSPGKIIN"
BASE COUNT          225 a          420 c          302 g          194 t
ORIGIN      
        1 ggcgagcgcg aaatctcctt cgagagcaac ccggccggac aggcgcagtt ctatttcatc
       61 agcacgcccg cgcacgcgaa acacccgacc gcccacgcca cgcgcgaaca aaacatcggc
      121 gcgcccatcg gcgacgccgc gaagtgcaat cgccgccgca taaataaata catccacgcc
      181 gatggcatca aaagctgcca gctcgtcatg gggttcaccg agttcgaacc cggcagcgtg
      241 tggaacacga tgccgacgca cacgcactca cgccggaccg agatttattt ctacttcgat
      301 ctcggcaacg agcttgtcat tcacctcctc ggcgaaccga cggcgacgcg ccacatcatc
      361 gttcgcgacc gcgaggtcgc cctctcgccc tcgtggtcga tccactccgg cgtcggcacc
      421 ggcagttacc gcttcatctg ggcgatgggc ggcgacaacc aaaccttctc cgacatggac
      481 gccgtcgcga tgaacgacct gcgatgacgc accgctgagt tttacgcgga ccaaccgaac
      541 accgatgacc tcacaaaaaa ccgaactggc gaccgtaggg cgggctttag cccgccgctt
      601 gtgaatcttc gccacgtcac aatgcccatc tcgactgcaa ggcgggctga agcccgccct
      661 acttgagccg ttgcgccgca tctcacttac tcgcccgctc cccctttcca tgcccaacat
      721 cctcgataaa ttccgcctcg atggtcgcgt cgcgatcgtc accggcgcgt cccgtggcct
      781 cggtgccggc atggcacgcg ctctcgccga agccggcgcc gacatcgcct tggtcgcccg
      841 cggcgacacc tctgcactcg tcagcgatat cgctgccctc ggtcgcaaat ccgtcaccgt
      901 cgccatcgat gtcgccgccg ccggcgccgc cgaaaaaatt gtcagcgata ccgtcgccgc
      961 gctgggccgc gccgacatcc tcatcaacaa cgccggcatc atccgccgcg cctccttcgt
     1021 cgatttcacg gagcaggatt ggcgcgaggt ggtcgacgtg aacctcgacg ccgcttttcg
     1081 cctgagccag gttttcgcgc ggcaattgat cgcaaaaaaa tcgccgggca agatcatcaa
     1141 c
//
LOCUS       DASPLT010001237         2246 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_38770, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001237 DASPLT010000000
VERSION     DASPLT010001237.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 2246)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2246)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2246
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_38770"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            182..994
                     /gene="modA"
                     /locus_tag="VEA63_12670"
     CDS             182..994
                     /gene="modA"
                     /locus_tag="VEA63_12670"
                     /inference="COORDINATES: protein motif:HMM:NF024921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0030973 - molybdate ion binding [Evidence
                     IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HYD84907.1"
                     /translation="MGSLRLFFHGIFSALLAATSSVTAAEPRPARDRETLSIAAAANL
                     VYALDAVNAAFHHTAPEISLTVATGASGSLVSQIEHGAPYDVLLSADVDYPRALIAAG
                     HAVPATLVTFATGRLVLWTTHPDIPLESIAATVRSERVRKLALAHLDTAPYGRAAQQT
                     LEALGAWTDAQSKIVIGENITQTAQFVDTRNADAGFVALSLVLSPKLRDRGRWLEVPA
                     ELHAPLDHAAVITQRGENNPAAARYLAFLRSKPAQEILQRFGYAVPRSSVSI"
     gene            1268..1963
                     /gene="modB"
                     /locus_tag="VEA63_12675"
     CDS             1268..1963
                     /gene="modB"
                     /locus_tag="VEA63_12675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HYD84908.1"
                     /translation="MPESASTFLGLSAPLWQSLGLTLKLAAITTLVLGALGLPLAHWL
                     NTTRRRVAPLVETLVTLPVVLPPTVIGFYLLVAFSPDRPPGSWWSLAFGAPLAFSFTG
                     LVIASVFYSLPFAVQPFQAALRGVPPELLDAGTALGATPLRVWLRVHIPLAWRGIAAG
                     LTLGFAHTLGEFGVVLMIGGSIPGVTRVASIALYDEVQKLNYSEAHAFAAVLLVVSFA
                     LLLIVTLLQRRRR"
BASE COUNT          338 a          889 c          589 g          430 t
ORIGIN      
        1 cgaagctttc gcgccggatt ccgcgcccca ggtaaaattg atttcaacaa agtatcagcc
       61 atgcactctc ttctttccac atcgcggcag gatcggggct caccctgaaa acactcgcgc
      121 gcccgcgtga gctcgctgag atgaaacgat gcgagcttca ccgtgcagaa atttttttgg
      181 cgtgggaagc ctccgtttgt tttttcacgg aattttttcc gccctgcttg ccgccacgag
      241 cagcgtaacc gcagccgaac cccgcccagc gcgcgaccgc gagacactct cgatcgccgc
      301 cgccgccaac ctggtctacg cgctcgatgc ggtgaacgcc gccttccacc acaccgcgcc
      361 tgaaatttca ctcaccgtcg cgaccggcgc atcaggcagc ctcgtttccc aaatcgaaca
      421 cggggcgccc tacgacgtgc tgctctccgc cgacgtcgac tacccgcgag cgctcatcgc
      481 tgcaggccac gccgtgcccg cgacgctcgt caccttcgcc accggccgcc tggtgctctg
      541 gaccacccac cccgacatcc cgctcgagtc gatcgccgcc accgttcgca gcgagcgcgt
      601 ccgcaaactc gccctcgctc acctcgacac cgccccctac ggccgcgccg cccagcaaac
      661 cctggaggcg ctcggtgcgt ggacagacgc gcaatcgaaa atcgtgatcg gggaaaacat
      721 tacgcagacc gcgcagttcg tggacacgcg caatgccgac gccggtttcg tcgcgctctc
      781 gcttgtgctc tcgcccaagc tccgcgaccg aggccgctgg ctcgaggtgc ccgccgaact
      841 gcacgctccg ctcgaccacg ccgccgtgat cacccagcgc ggcgaaaaca atcccgccgc
      901 cgcacgctac ctcgcgttcc tccgcagcaa acccgcgcag gaaatcctcc aacgcttcgg
      961 ctacgcagtg ccgcgatcga gcgtttcaat ttagcccaac cgcgcattcc ggcacggcag
     1021 cgcctcacgc cccgaacacc gccaaccctg acccgcattc atcacccgca gatgcgaggt
     1081 gcgcgcagtc tgttgtaacg aaacgcgtga gcgtttcgtc cgggcgagag acgaaatgct
     1141 cacgcttttc gctgcaggtg tgaatctccg tgctaatcgc gctggtttcg ccggctccaa
     1201 attcgacgcc agacgttcaa agttcaaagt tggacgttca acgttcaccg ttcagttttc
     1261 cgccgccttg cccgaatccg cctccacctt cctcggtctc tccgcgccgc tctggcagtc
     1321 gctcggcctc accctgaaac tcgccgcgat caccacgctc gtgctcggcg cactcgggct
     1381 cccgcttgcg cactggctca acaccacccg ccgtcgcgtc gcgccgctcg tcgaaacgct
     1441 cgttacgctt ccggtcgtgc tcccgccgac cgtcatcggt ttctatttgc tcgtcgcctt
     1501 ctcgccggat cgcccgcccg gcagttggtg gagtttggcc ttcggcgccc cgctcgcctt
     1561 ctcgttcacc ggcctcgtca tcgcctcggt gttctactcg ctgccgttcg ccgtgcagcc
     1621 gtttcaagct gcgctccgcg gcgtcccgcc cgagctcctc gacgccggca cggcgctcgg
     1681 cgctacgccg ctgcgggtgt ggttacgcgt tcacatcccg ctcgcctggc gcggcatcgc
     1741 cgccggcctc acgctcggct tcgcgcacac cctcggcgag ttcggcgtcg tgttgatgat
     1801 cggcggctcc atccccggcg tcacccgcgt cgcgagcatc gccctctacg acgaggtgca
     1861 aaaactcaac tactccgaag cccacgcctt cgccgccgtc ctgctcgtcg tgtctttcgc
     1921 gctcctcctg atcgtcaccc tcctccaacg ccgccgccgc tgaagccggt ccgtcgcata
     1981 ctgcccgcac cgcagccctc gacgcggcgc caactttgaa cattggattt tctctggagc
     2041 ttggagcttg gtccttggat ctcggtcctt ggagcttctc ctcccccgcc ctcttgacga
     2101 cttcggcata atacccgctg ctcacaccgc acgtcgcatg tccgccgcca cgctcaacac
     2161 caacccgccc tcgccgttcc gccgcctcgt cgcggcgctc ggcgtcgcgc tggtgttgct
     2221 gctcggcgtc gccgccgtca accccg
//
LOCUS       DASPLT010001238         1016 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_199842, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001238 DASPLT010000000
VERSION     DASPLT010001238.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1016)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1016)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1016
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_199842"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1016
                     /locus_tag="VEA63_12680"
     CDS             <1..>1016
                     /locus_tag="VEA63_12680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009507757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HYD84909.1"
                     /translation="AQCELEVEVGERIQAMIPSAELVRFNNSATEVVMVAFRVARAHT
                     KRPLILRFEGHYHGWSDEGLPGFVNPPGSWGDDESPANVHPSMGVIPAVPQQFVVARW
                     NDPEHLRRRVEQFRGQIAAIVFEPVLCNTCCIEPVPGMMETIRELCDRDGMLMIADET
                     ITGFRFGAAGAQGYLGVKPDLTILGKAVGGGTPVAALAGTRAAMRTILSGEVIHAGTL
                     NGNPLCLAASKWSLDEILARKATHPAVIQKLGRRLMTGLSELAREHGVPLRPQGPGLI
                     FHTVMLKDNAAEGPIRDYRDYVRRHHAPRWMHLRRCLLEAGVRAIERGLWSISLSHRD
                     ADID"
BASE COUNT          182 a          292 c          352 g          190 t
ORIGIN      
        1 gcgcaatgcg agttggaagt ggaagtcgga gagcgcatcc aggcgatgat cccgtcggcg
       61 gagttggtcc gtttcaacaa ctcggcaacg gaagtggtga tggtggcctt ccgcgtggcg
      121 cgggctcaca cgaagcgtcc gttgatcctg cggttcgaag ggcactatca cggttggagc
      181 gacgaggggc tgccggggtt tgtgaatcct ccgggaagtt ggggcgacga cgagtcgccg
      241 gcaaacgtgc acccgagcat gggtgtgatt cccgccgtgc cgcagcagtt tgttgtcgcc
      301 cgatggaacg atccggaaca cctgcgccgc cgcgtcgaac aattccgcgg acagatcgcg
      361 gccatcgtat tcgaacccgt gctttgcaac acctgctgta tcgagccggt gccggggatg
      421 atggagacga tccgcgagct gtgcgatcgc gacggcatgc tgatgatcgc ggacgagacg
      481 atcacgggtt ttcgtttcgg cgcggcgggc gcccaaggtt atttgggcgt gaagcccgat
      541 ctcacgattc tgggaaaagc ggtgggaggc ggcacgcccg tggcggcgct tgccggcacg
      601 cgcgctgcga tgaggaccat tctttccggc gaggtgattc acgccggcac gctcaacggc
      661 aatccgttgt gtctcgcggc gagcaagtgg agcctcgatg aaattctcgc gcggaaagcc
      721 acgcatccag cggtcattca gaagctcggc cgccgcctaa tgacaggcct gtcagagctc
      781 gctcgcgagc acggcgtgcc cttgcgtccg cagggaccag gactgatttt ccacaccgtg
      841 atgctcaagg ataacgctgc cgaggggccg attcgcgact accgtgacta tgtgcggcgg
      901 caccacgcgc cgcgttggat gcatttgcgc cgttgtttat tggaggcggg cgtgcgagcg
      961 atcgaacgcg ggctctggtc aatcagtctg tcgcatcgcg acgcggacat cgacga
//
LOCUS       DASPLT010001239         2359 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_239026, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001239 DASPLT010000000
VERSION     DASPLT010001239.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 2359)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2359)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2359
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_239026"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..547)
                     /locus_tag="VEA63_12685"
     CDS             complement(<1..547)
                     /locus_tag="VEA63_12685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HYD84910.1"
                     /translation="MSSPSDTSIDPYAPVPSFLERHADRASAVVVLVLTAVLTVLSFP
                     PFHTPEFAYAMLVPGIFWAYRKPTLKLYFGTLVAAQAVAWTIMLGWLHHVTWVGLLLL
                     GPVIGAWVSVWYLAAWWAMPRMLGRPALTRMVGMVGLAGAWVVIEWTRTWVLSGFPWL
                     PLSVSQWERSSILQVAAFTGAW"
     gene            complement(581..>2359)
                     /locus_tag="VEA63_12690"
     CDS             complement(581..>2359)
                     /locus_tag="VEA63_12690"
                     /inference="COORDINATES: protein motif:HMM:NF013117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel domain-containing
                     protein"
                     /protein_id="HYD84911.1"
                     /translation="ASANPTEKERLDEKLTRLRRELAILDERQVLDERERQLRSERRE
                     RTLDVLREKLRAIDVIPEQVERRLSELATRRRNALDDREDLASAVAAARARATEKTND
                     REAEIEERLFTRNEELRALALEREAAEAELDLAQQSQQLRGQLKTADAGVSESGLRPL
                     FEAYTRVRQTRKSSDRFRALVEDLESSRAVSEASLELSRQKLARFDEELTLLEKQTGF
                     FVRDPRIERLLATQRSQKAALSERLPFMIRQVAALTDAQTALRQQQELAELSARNDEE
                     HYAAVRSHYLGRLRWPAVLVAALLLLHVLVGYILLPLAFRNEALFMARRIARYIVVVC
                     ATGIVAGFLFDDLSMIAATLGIVSAALVISLQDVCTSVFAWFVIMTGEKFRIGDRLEI
                     DGTRGDVLDLQLLRTTLLEVTGTDDQPTGRVILVPNNVLFKTKIFNFTHEHPFIWGKL
                     SVTVAFSTPMAGALVLFQRILEEETRESFEAARSAATKLQRRYGVEDAIYEPHIQTEI
                     VNDGVTLTLLFVAHYRQFSATRNRLNRRIIRELESHPHVQLSVQQIQIVPNTPATAPA
                     AMLGPELTRPPFAFGADSTVADGLRS"
BASE COUNT          451 a          834 c          669 g          405 t
ORIGIN      
        1 cccacgcgcc cgtgaacgcc gccacttgca ggatgctcga acgctcccac tggctcaccg
       61 acaacggcag ccacggaaaa ccgctcaaca cccacgtgcg ggtccactcg atcacaaccc
      121 acgcgcccgc gagcccgacc attccgacca tacgggttaa cgccggtcgc ccgagcatcc
      181 gcggcatcgc ccaccacgct gcgagatacc acacgctcac ccacgcgccg atcaccggcc
      241 caagcagcag cagcccgacc cacgtcacat gatgcagcca acccagcatg atcgtccacg
      301 cgaccgcctg cgccgccacg agcgtgccga aataaagttt cagggtcggt ttccgatacg
      361 cccaaaaaat tcccggcacg agcatcgcat acgcgaactc cggcgtgtga aacggcggaa
      421 acgacagcac cgtcagcacc gccgtcagca ccaacaccac cacggcggac gcccgatcgg
      481 cgtggcgttc taaaaacgac ggcaccgggg cgtaaggatc gatggaggtg tcagaaggcg
      541 aggacatgaa ccgccaacct cgcccaacct cgctcggcgc tcaagagcga aggccatccg
      601 ccacggtcga atccgcgccg aacgcgaacg gcggccgcgt cagctccggt ccgagcatcg
      661 ctgcgggcgc cgtcgcaggc gtgttcggca cgatctgaat ctgctgcacc gacaactgga
      721 cgtgtggatg ggactccagc tcccgaatga tccggcgatt cagccggttt cgcgtcgccg
      781 aaaactggcg gtaatgcgca acgaaaagca gggtgagcgt cacgccatcg ttcacgattt
      841 ccgtctgaat atgcggctcg taaatcgcgt cctccacgcc gtagcgacgc tggagcttgg
      901 tcgccgcact gcgggcggct tcgaaacttt cccgcgtctc ctcctcgaga attcgttgaa
      961 acagcaccag cgcccccgcc atcggcgtcg aaaacgccac tgtcacagag agctttcccc
     1021 aaatgaacgg atgctcgtgc gtgaagttaa agatcttcgt tttaaacagg acgttgttcg
     1081 gcacgagaat gacccgtccc gttggctggt cgtccgtgcc cgtcacctcg agcagcgtcg
     1141 tgcgcagcag ctgaagatcc agaacgtcgc cgcgcgtgcc gtcgatttcg agccggtctc
     1201 caatccggaa tttttctccg gtcatgatca cgaaccaggc gaagacggag gtgcatacgt
     1261 cctgaaggga aatcaccagc gccgcgctca cgatgccgag cgtcgccgcg atcatcgaca
     1321 gatcgtcgaa cagaaacccg gccacgatgc ccgtcgcgca caccacgacg atatagcgtg
     1381 cgatgcgacg cgccatgaag agcgcttcgt ttctaaacgc cagcggcagc agaatgtagc
     1441 cgaccagcac gtgcagcaga agcagtgccg cgaccagcac cgccggccag cgcaaccggc
     1501 cgagatagtg gctccgcacc gccgcgtagt gctcctcgtc gtttcgcgcg gacaactcag
     1561 ccagctcctg ctgctgacgc aacgcggtct gcgcgtcggt cagcgcggcg acttgccgga
     1621 tcatgaaggg cagtcgttcg ctcaaggccg ctttctggct tcgctgggtg gcaagcagtc
     1681 gttcgatccg gggatcgcgc acgaaaaatc cggtctgctt ttcgagcagt gtcagctctt
     1741 cgtcgaaccg cgccagtttc tgccggctga gttccagcga cgcctcgctc accgcccggc
     1801 tgctttccaa atcctcgacg agcgcacgaa agcggtcgct cgattttctc gtttggcgca
     1861 ctcgcgtgta ggcctcgaac aaaggacgca agcccgactc gctgacgccg gcatccgccg
     1921 tcttcaattg tccacggagc tgctgcgatt gctgcgcaag atccagctca gcttccgccg
     1981 cttcgcgttc gagcgcgagc gctctcagct cctcgttcct cgtgaagagt ctttcctcga
     2041 tctcggcttc ccggtcgttc gtcttctccg tcgcacgggc ccgcgccgca gcgaccgccg
     2101 atgcgaggtc ctcccgatca tcgagcgcgt tccgccggcg cgtcgcgagt tcgctcagcc
     2161 gccgctcgac ctgctcggga atgacatcga tcgcgcgcag tttttcgcgc aacacgtcga
     2221 gcgtgcgttc ccgccgctcc gagcgaagct gccgttcgcg ctcgtcgagc acctgtctct
     2281 cgtcgagaat cgccaactcc cgccggagcc gcgtcagctt ttcgtccaag cgctccttct
     2341 ccgtcggatt cgcgctcgc
//
LOCUS       DASPLT010001240         1340 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_260450, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001240 DASPLT010000000
VERSION     DASPLT010001240.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1340)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1340)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1340
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_260450"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..708)
                     /locus_tag="VEA63_12695"
     CDS             complement(<1..708)
                     /locus_tag="VEA63_12695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin-like FloA family protein"
                     /protein_id="HYD84912.1"
                     /translation="MNLTLILLIVLGVAALIVFGLVVSFFSVWLRALLAGAPVSIFNL
                     VAMRLRQVPYSVMVDARIRATKAGINLSIDDIEAQYLAGGNVIACVQALIAAQKAGIA
                     LDWQRACAIDLATKGSGKSVEEAVRTSVDPKVIDCPNPEGGRVTIDGVAKDGIQVKVK
                     ARVTVRTNLDRFVGGAKEDTIIARVGEGIVSTIGSSESYKSVRESPDAISKTVLHRGL
                     DVGTAFEILSIDIADVDV"
     gene            complement(714..1181)
                     /locus_tag="VEA63_12700"
     CDS             complement(714..1181)
                     /locus_tag="VEA63_12700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HYD84913.1"
                     /translation="MTTIVLLFALGIVLLTLEVVVPGGVLGVIGGLLMLGGCVLAFNE
                     FGVEGGGLATVVALACLALGLYVEFRLLPRTRFGKKLFLSNSVDARSQPLPADATAVV
                     GKTAEALTTMAPSGYVAIDGRRYEARSQAGLIAKGTTVRVVGVDNFHLIVTHT"
BASE COUNT          277 a          490 c          345 g          228 t
ORIGIN      
        1 gacgtccacg tccgcgatgt cgatcgacaa aatctcaaac gccgtgccca catcgagacc
       61 gcgatgcagc accgtttttg aaatggcatc ggggctctcg cgcaccgatt tatagctctc
      121 cgacgaaccg atcgtcgaaa cgatgccttc acccacgcgc gcgatgatcg tgtcctcttt
      181 cgcgccgccg acgaaacgat cgagattcgt gcggacggtc acgcgcgcct tcaccttcac
      241 ttgaatgccg tcctttgcga cgccatcgat cgtcacacgg ccgccttccg gattcggaca
      301 atcgattacc ttcggatcga ccgatgtgcg caccgcttcc tcgaccgatt tgcccgatcc
      361 tttcgttgcg agatcgatcg cgcacgcccg ttgccagtcg agcgcgatgc ctgccttttg
      421 cgccgcgatg agcgcctgca cgcacgcgat cacgttgccg cccgcgagat actgagcctc
      481 gatgtcgtcg atcgagagat tgatgccggc cttcgtggcg cgaatacgcg catccaccat
      541 cacgctgtag ggcacctgcc gcaggcgcat cgcgacgagg ttgaaaatgc tcaccggcgc
      601 acccgccaac aacgcgcgta accacacgct gaagaatgag acgacgaggc cgaaaacgat
      661 cagcgccgcg acgccaagca caatgagaag aatcagggtc aggttcatgg gaatcaggta
      721 tgggttacga tcaaatgaaa gttatcgacg ccgacgacgc gcaccgtcgt gccctttgcg
      781 atcagaccag cctgcgacct cgcctcgtag cgacgcccgt cgatcgcgac atagccgctc
      841 ggcgccatcg tcgtgagcgc ctccgccgtc ttgcccacca ccgccgtcgc atccgccggc
      901 aacggctggc ttcgagcatc caccgagttc gagagaaaaa gttttttacc gaaacgagtc
      961 cgcggcaaca gtcgaaactc cacgtagagt cccagtgcca aacacgcgag cgccaccacc
     1021 gtcgccagcc cgccgccctc caccccaaac tcgttaaacg ccagcacgca tccgccgagc
     1081 atcaacagcc cgccgatcac gcccagcacg ccgcccggca ccacgacttc gagcgtcagc
     1141 agcacgattc ccaacgcaaa gagaagcacg attgtcgtca tgattgcgcc ttctccacca
     1201 ccaacccgaa gtcgtgccgg cgtcgcacta cgaccaccgt tcccgccgcg acgtcgccga
     1261 tttccaccgt cgcctcgtag cgttttccgc cgacctccac ttcgccgccc ggacgcaacg
     1321 ccgtcaccgc gattccccgc
//
LOCUS       DASPLT010001241         1090 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_15585, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001241 DASPLT010000000
VERSION     DASPLT010001241.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1090)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1090)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1090
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_15585"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(17..>1090)
                     /locus_tag="VEA63_12705"
     CDS             complement(17..>1090)
                     /locus_tag="VEA63_12705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HYD84914.1"
                     /translation="INADYFNYSQIVMRNTRLSVIADVLGSFGALIVLGYGGLLVVQG
                     AMEIGTLVAFNAYIVFIFPPIVRFVDLTAVFQRANTALENMWAMLDTRPDVTDRPGAG
                     ALAAIRGEVEFRNVCFDYELEPPGQGRPRTLTDVNFHIPAGKVVAIVGPSGSGKSTII
                     NLVARFYDVASGSVLIDGSDVRGVTVESLRRQIGIVLQENVLFSGTLEDNIKYGRTDA
                     TPEEIRAAADAANAHEFIVKLPDGYATVVGERGAKLSGGQRQRIAIARAILRDPRILI
                     FDEATSALDTVSERLIQQAMERLMKGRTAFIIAHRLSTIQRADVILVMEQGRLAEKGT
                     HAELLAAGGLYARLHALQFQEAG"
BASE COUNT          207 a          428 c          269 g          186 t
ORIGIN      
        1 cccaacgcct ccaagcctac cccgcctcct gaaactgcaa cgcgtgcaat cgcgcataca
       61 gcccccccgc cgcgagcagc tccgcatgcg tgcccttctc cgccagcctt ccctgttcca
      121 tcaccaaaat tacgtccgcc cgctggatcg tcgacaaccg gtgcgcgatg ataaacgccg
      181 tccgcccttt catcagccgc tccatcgcct gctggatcaa ccgctccgac acggtgtcga
      241 gcgcactcgt cgcctcgtca aaaatcagaa ttcgcggatc gcgcagaatc gcccgcgcaa
      301 tcgcgatccg ctgccgctgc ccgcccgaca acttcgcgcc gcgctcgccc accaccgtcg
      361 cgtatccatc cggcaacttc acgatgaact cgtgcgcgtt cgctgcgtcc gccgccgcgc
      421 ggatctcctc cggcgtcgca tccgtgcggc cgtatttgat gttgtcctcc aacgtccccg
      481 aaaacagcac gttctcctgg agcacgatgc cgatctgtcg ccgcagcgat tccaccgtca
      541 cgccgcgcac gtcgctcccg tcgatcagca cgctgcccga cgccacgtcg tagaagcgcg
      601 ccacaagatt gatgatcgtg ctcttgcccg atccgctcgg cccgacgatc gccaccacct
      661 tccccgcagg aatgtgaaaa ttcacgtccg tcaacgtccg cggccgacct tgccccggcg
      721 gctcgagctc gtagtcgaaa cacacattgc gaaactccac ctcgccacgg attgccgcca
      781 gcgccccggc tcccggccga tccgtcacgt ccggcctcgt gtcgagcatc gcccacatgt
      841 tctccaacgc cgtattcgcg cgctgaaaca ccgccgtcaa atccacgaag cgcacgatcg
      901 gcggaaagat gaagacgatg taggcattga acgccaccag cgtgccgatc tccatcgcgc
      961 cctgcaccac cagcagtccg ccgtaaccga gcacgatcaa tgcgccgaac gagccgagca
     1021 cgtccgcgat caccgacagc cgcgtgttgc gcatcacgat ctgcgagtag ttgaaataat
     1081 ccgcgttgat
//
LOCUS       DASPLT010001242         1592 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_280707, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001242 DASPLT010000000
VERSION     DASPLT010001242.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1592)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1592)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1592
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_280707"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1548)
                     /gene="acnA"
                     /locus_tag="VEA63_12710"
     CDS             complement(<1..1548)
                     /gene="acnA"
                     /locus_tag="VEA63_12710"
                     /EC_number="4.2.1.3"
                     /EC_number="4.2.1.99"
                     /inference="COORDINATES: protein motif:HMM:NF006757.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HYD84915.1"
                     /translation="MSENGFGKPAADFTKTFHVEGSGWTRPGGGSQELVPESDRDVKN
                     TSVTTEREMANNRPTPDPVATQAGRIDGEISHGSVLIAAITSCTNTSNPSVMLAAGLL
                     AKKAVERGLRVAPTVKSSLAPGSRVVTDYLTKTGLQTYLDRLGFQTVGYGCTTCIGNS
                     GPLHPAIEEAVVKNDLVAASVLSGNRNFEARVHQNIKSNFLMSPPLVVAFALAGRVDI
                     DMTSEPIGRDQTGQPVFLKDLWPTLQEVRDQLQAALKPEVFRKLYSDFAEQNPKWNEI
                     PSTTGNVYQFDAKSTYIQEPPFFSKFGMQPGTIEEITGARALGIFGDSVTTDHISPAG
                     AIKKTSPAGKFLIENGVPFEDFNSYGSRRGNDRIMTRGTFANVRIKNLMLGGEEGGNT
                     IYQPTGERMSIYDAAVKRMANNTPLIIIAGQEYGTGSSRDWAAKGTNLLGVKVVVAQS
                     FERIHRSNLVGMGVLPLQFRPGETLETLGITGRESFAISGLDRLSPHADVTVEATGDD
                     GRVQRFEA"
BASE COUNT          270 a          559 c          462 g          301 t
ORIGIN      
        1 ggcctcgaac cgctgcacgc ggccgtcgtc accggtcgcc tcgaccgtga cgtcggcatg
       61 gggtgagagc cggtcgagac cactgatcgc aaacgactcg cgtccggtga tcccgagcgt
      121 ctcgagcgtc tcgccgggcc ggaactggag cggcagcacg cccatgccga cgaggttcga
      181 gcgatggatg cgctcgaagc tctgcgccac gaccactttc acaccgagca gattcgtgcc
      241 cttcgccgcc cagtcgcgcg acgaaccggt gccgtattcc tggcccgcga tgatgatcaa
      301 cggcgtgttg ttcgccatgc gcttcaccgc cgcgtcgtag atcgacatcc gctcgcccgt
      361 cggctggtag atcgtgttcc cgccttcttc gccgccgagc atcaggttct tgatgcgaac
      421 attcgcgaac gtcccgcggg tcatgatgcg atcgttaccg cgccgtgatc cgtaagaatt
      481 gaagtcctcg aacggcacgc cgttctcgat gaggaacttt ccggccggcg acgtcttctt
      541 gatcgcgccc gcaggcgaga tgtggtccgt cgtcaccgag tcgccgaaaa tcccgagcgc
      601 acgcgcgccc gtgatctcct cgatcgttcc cggctgcatg ccgaacttcg agaagaacgg
      661 cggctcctgg atgtaagtcg atttcgcgtc gaactggtaa acgttgcccg tcgtcgacgg
      721 gatctcgttc cacttcggat tctgctccgc gaaatcgctg tagagcttgc ggaaaacctc
      781 cggcttcaac gccgcctgca actggtcacg cacttcctgc aacgtcggcc acaggtcctt
      841 caggaaaacc ggctgcccgg tctggtcacg gccgatcggc tcgctcgtca tgtcgatgtc
      901 gacgcgccct gcgagggcga acgccaccac cagcggcggc gacatgagga agttcgactt
      961 gatgttctgg tgcacgcgcg cctcgaagtt gcggttgccc gaaagcaccg acgccgccac
     1021 gagatcgttc ttcaccaccg cttcctcgat cgccggatgc agcggacccg agttgccgat
     1081 gcacgtcgtg caaccgtagc cgaccgtctg aaaaccgagc cggtcgagat acgtctgcaa
     1141 acccgtcttc gtcagataat ccgtcaccac gcgactgccc ggcgccagcg acgacttcac
     1201 cgtcggcgcc acgcgcagtc cgcgctcgac cgccttcttc gccagcaaac ccgccgcgag
     1261 catcaccgaa ggattcgacg tgttcgtgca gctcgtgatc gccgcaatca acacgctgcc
     1321 gtggctgatc tcgccgtcga tgcgccccgc ctgcgtcgcc accggatccg gcgtcgggcg
     1381 attgttcgcc atctcgcgct cggtcgtcac gctcgtgttc ttcacgtcgc ggtccgattc
     1441 cggcacgagt tcctgactac cgccgccggg ccgcgtccaa ccgctgccct cgacgtgaaa
     1501 cgtcttcgta aaatccgccg cgggcttgcc gaatccattc tcgctcacgc cacgagaaaa
     1561 tgccgtgacg aactcgcgct tgatcgcagg ca
//
LOCUS       DASPLT010001243         1019 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_179592, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001243 DASPLT010000000
VERSION     DASPLT010001243.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1019)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1019)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1019
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_179592"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(150..>1019)
                     /locus_tag="VEA63_12715"
     CDS             complement(150..>1019)
                     /locus_tag="VEA63_12715"
                     /inference="COORDINATES: protein motif:HMM:NF024743.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HYD84916.1"
                     /translation="INVANGDLHIRGTDAAEITLRTDLKPEKSDVREDGLRVITTSSS
                     YSLLEKDNVVTLSHGSEGWPGNSSDFDITVPLNTSVVIANAFGGDIRIENVTGDIEVK
                     SLNGEVKLDGVAGGALVETMNGEIDVNVRTLVDNKPLSFTSLNGEVALRVPTDAKANV
                     RLRTHNGTILTDFPETQLITKTATLKGESGHGAGAIDEDIRATVRDAVRVGMEAAREA
                     TRAARDAMREARENGHVNVDVNLDGMTIPVPPMPPLPPMTGGKIVTGTLNGGGPEILI
                     STMNGDVTLRKAK"
BASE COUNT          152 a          273 c          379 g          215 t
ORIGIN      
        1 gggcggctac gacctcggcg agaggatcaa agacgggctg cgtttgtcgc agcacgtcgt
       61 tgcgcgcgag cgcgccacct acaaacagag caggcaggca caaggcctgc cgctttggga
      121 gggtggggtg gcgaagacgg ctgccccggt tacttcgcct tccggagtgt gacgtcgccg
      181 ttcatcgtgc tgatgaggat ttcggggccg ccgccgttga gcgtgccggt gacgatcttg
      241 ccgccggtca tcggaggaag cgggggcatg ggcgggaccg ggatggtcat cccgtccaga
      301 tttacgtcga cgttgacatg gccgttttca cgggcctcgc gcatcgcgtc gcgggcggcg
      361 cgagtggctt cgcgagcggc ctccatgccg acgcgaacgg cgtcgcgcac ggtggcacgg
      421 atatcttcgt cgatggcgcc cgcgccgtgg cccgactcgc ctttgagcgt ggcggttttg
      481 gtgatgagtt gagtctccgg aaaatccgtg aggatggtgc cgttatgggt gcgaaggcgg
      541 acgttggctt tggcgtcggt gggaacgcga agggcgacct cgccgttcaa tgaggtgaac
      601 gagagcggct tgttgtccac gagggtgcga acgttgacgt cgatttcgcc gttcatcgtt
      661 tcgacgagtg cgccgccggc gacgccgtcg agtttcactt cgccgttgag gcttttcact
      721 tcgatgtcgc cggtgacgtt ttcgatgcgg atgtcaccgc cgaaggcgtt cgcgatgacg
      781 acgctggtgt tgagtggcac ggtgatgtcg aagtcgctgc tgttacccgg ccagccttcg
      841 gagccgtggc tgagggtcac gacattatct ttttcgagca gcgagtagct ggaggaggtg
      901 gtgatgacgc gcaggccatc ttcgcggacg tcggacttct cgggcttgag gtcggtgcgg
      961 agggtgatct cggcggcgtc ggtgccgcgg atatgaaggt caccgttggc aacgttgat
//
LOCUS       DASPLT010001244         1005 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_15626, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001244 DASPLT010000000
VERSION     DASPLT010001244.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1005)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1005)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1005
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_15626"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..240)
                     /locus_tag="VEA63_12720"
     CDS             complement(<1..240)
                     /locus_tag="VEA63_12720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD84917.1"
                     /translation="MSLLALGSVFVIVGMRRAGVFSQPSGLIVAGGPFLIVVYAAADF
                     PTHNPAVLTTACIVLVLTTRWVGLDRPKHARTPIAL"
     gene            complement(237..>1005)
                     /locus_tag="VEA63_12725"
     CDS             complement(237..>1005)
                     /locus_tag="VEA63_12725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD84918.1"
                     /translation="LWIGITRRRSAQLLLVAIAINGVALAVVAILQRLTESDRLLFFW
                     PRPTVYFVASFISKNHAGAYLNLILALCLGLAWWHADRAEQRMAKSHPGLLFLFLAVF
                     VAVALLFTYAVAATALGVAFVLAVAIGYITQRIFHAKHSGSSRGATLLTAGLCAVFLG
                     LCAYSLNIEKVSRKFRRVSDEEYTSSITYSSRPKPPSTWAATSSGSVTASAAIATCFR
                     ASNKLTRKSSPAENVASTGSTRTTTTWNSSPKAAFSA"
BASE COUNT          222 a          251 c          372 g          160 t
ORIGIN      
        1 tagtgcaatt ggggttcgcg cgtgcttggg tcgatcgagg ccgacccagc gagttgtcaa
       61 cacgaggacg atacaagcgg tcgttagcac ggcgggattg tgcgtcggaa agtcagcggc
      121 ggcgtaaacc acgatcagga acgggccgcc ggcgacgatg agccccgagg gttgcgaaaa
      181 gacgcccgca cgacgcatgc cgacgattac gaagacggat ccgagagcga gcaggctcaa
      241 gccgagaacg ccgctttcgg cgaagaattc cacgtagtcg ttgtgcgcgt gctcccagta
      301 gaggcgacgt tttcggctgg tgaggatttc cgggtaagct tgttggaagc gcggaaacac
      361 gtagcgatag ccgccgatgc cgtgaccgag ccagagctcg tcgcggccca tgtcgaaggt
      421 ggctttggtc gcgagctgta agtgatcgag ctggtgtatt cctcgtcgga gacgcgcctg
      481 aatttgcgag agactttttc gatgttgagc gagtaagcgc agaggccgag aaagacggcg
      541 cagaggccgg cggtgagcag cgtcgcgccg cgtgacgagc cgctgtgttt ggcgtggaag
      601 atgcgctggg tgatgtagcc gattgcgacg gccaggacaa aagcgacgcc gagcgcggtg
      661 gccgcgactg catacgtgaa gagcaaggcg acggcgacga aaacggcgag aaagagaaaa
      721 agtagaccgg ggtgggattt cgccatgcgt tgctcggcgc gatcggcgtg ccaccaggcg
      781 aggccgagac acagagcgag aatgagattc aggtaagcgc cggcgtggtt tttggagatg
      841 aacgacgcga cgaaatacac ggttggccgc ggccagaaaa agagcagacg atcgctctcg
      901 gtgaggcgtt gcaggatggc gacgacggcg agggcgacgc cattgatcgc gatcgcgacc
      961 agcaggagtt gtgcgctgcg gcgacgggtg atgccgatcc agagc
//
LOCUS       DASPLT010001245         1319 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_199925, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001245 DASPLT010000000
VERSION     DASPLT010001245.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1319)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1319)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1319
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_199925"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..227
                     /locus_tag="VEA63_12730"
     CDS             <1..227
                     /locus_tag="VEA63_12730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009510959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="rRNA methyltransferase"
                     /protein_id="HYD84919.1"
                     /translation="DMVEEPHHVMRHIVEPWLAHGWCRNFVVNLKFGRVDAVALLKEL
                     RGAESAFARRGGAFAVRHLYHDREEFTVVG"
     gene            416..1159
                     /locus_tag="VEA63_12735"
     CDS             416..1159
                     /locus_tag="VEA63_12735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009509838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HYD84920.1"
                     /translation="MKNKEITICGLAAVRAQFASEPGAIRRLFFDDATGRKIGVICKA
                     LAHAKKVYRRVEPVELEKIAGTIHHGGIVAVIAAGELRAPAAVELREWARRGEAIVVL
                     DRVGNAHNLGAIARTAAFFGVPRLVLPDDPAAAVPGEAAFRVAEGGLAHVEVLRVKML
                     AGFLRELAAAGYDVIGAAARGGRPQAVAANAKAKPVALVLGNEEHGLAPDVAAACTRL
                     VTIPGSGKVESLNVSVAAAVLLWELVARQ"
BASE COUNT          230 a          307 c          537 g          245 t
ORIGIN      
        1 gcgacatggt ggaggagccg catcatgtga tgcggcacat cgtggagccg tggctggcgc
       61 acgggtggtg ccggaatttc gtggtgaacc tgaagttcgg gcgggtggat gcggtggcgt
      121 tgctgaagga attgcgcggc gcggagtcgg cattcgcgcg gcggggcgga gcgtttgcgg
      181 tgcggcatct ttatcacgat cgcgaggagt tcacggtggt gggatgagcg gaagaatttt
      241 gggctaaagg aatgcgagaa ccacgaatgg acacgaaggg acacgaatat cgaagggcga
      301 gtagggcggc ttcgttaaac agaaacgagg cgcgatgccg ccgagccgtg ggtttttatt
      361 cctcgctcgt cctgcgcctt tgtaatcgga atttcgggca acgccgcatc gcaccatgaa
      421 aaacaaggag atcacgattt gcgggctggc ggcggtgcgg gcgcagttcg cgtcggagcc
      481 gggggcgatc aggcgattgt ttttcgatga tgcgacgggc cgaaagatcg gcgtgatttg
      541 caaggcgctc gcgcacgcga agaaagttta tcgccgcgtc gagccggtgg agttggagaa
      601 gatcgcaggc acgattcatc atggcggcat cgtggcggtg atcgcggcgg gtgaattgcg
      661 cgcgccggcg gcggtggagt tgcgcgagtg ggcgcgacgc ggggaggcga tcgtcgtgct
      721 cgatcgcgtg ggcaacgcgc acaacctcgg ggcgattgcg cggacggcgg cgttttttgg
      781 ggtgccgcgg ctggtgttgc cggatgatcc ggcggcggcg gtgccgggcg aagcggcgtt
      841 tcgcgtggcg gagggcggac tcgcgcatgt ggaggtgttg cgcgtgaaaa tgctcgcggg
      901 atttttgcgt gagctggcgg cggcgggtta cgatgtgatc ggtgcggcgg cgcgcggggg
      961 gcggccgcaa gcagtagcgg cgaatgcgaa agcgaagccg gtggcgctgg tgttggggaa
     1021 cgaggaacac gggctcgcgc cagacgtcgc ggcagcgtgc acgcgactgg tcacgattcc
     1081 cgggagtggg aaagtggagt cgttaaatgt gtcggtggcg gcggcggtgc tgttgtggga
     1141 actcgtggcg cgtcagtagt cgtcgagcac aaaccggacg atccaagtgc ggatcgtggg
     1201 aaggagaaga tccgagtttt ggaaccgctg acctgcgctg attttcagag gcggctcgtg
     1261 agaacgaatg agtgcgtgct tcgcctgatc ggcgaagcgc ggacgcttcg tcgggattc
//
LOCUS       DASPLT010001246         1204 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_38884, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001246 DASPLT010000000
VERSION     DASPLT010001246.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1204)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1204)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1204
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_38884"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            70..228
                     /locus_tag="VEA63_12740"
     CDS             70..228
                     /locus_tag="VEA63_12740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD84921.1"
                     /translation="MLTIMQTNADDDEGSGCIKYFSPRGLYVGTMCFSVPPFDSLHRH
                     LRFSSRDQ"
     gene            629..>1204
                     /locus_tag="VEA63_12745"
     CDS             629..>1204
                     /locus_tag="VEA63_12745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD84922.1"
                     /translation="MPSEPDPSAAAPLSFFPASTSPRPYSTEGRAAEEAIAAAGRVVE
                     QILGDAPLLALLAARGIGSTEVQRGAALHILAQQACETCQGHLGALDAAHEARALALA
                     SAREDYLDFRRAVKAACAHDSVRDPLEASVNPHQSLRAFVVQATASYLAALKTLPPAL
                     IAQHGFNLTRTNAALARVQRLVVLDTLFNTRA"
BASE COUNT          223 a          433 c          321 g          227 t
ORIGIN      
        1 tgttacacag agatcacgga gcgccgacac ggaggaagca ggaggccaca aggtgttggg
       61 aggtctttca tgctgacgat catgcagacg aatgctgacg acgatgaagg ctcgggttgc
      121 atcaagtatt tctctccgcg cgggctctat gtcggaacca tgtgcttctc cgtgccgccg
      181 tttgattcgc tccaccgaca ccttcgtttt tcttcgcgcg accaataaag cgcggcatcg
      241 ctctccgtcg ctgctcctac gcgcccgcgc cacgttgaaa acaagagggc ggaccgccta
      301 cccctaagcg gtccgcccat tatttgcttg gttaccccaa aatgcctccc cgctaggcgg
      361 gcgagacaca ccgttgttta gcacacccca agccaactcg caacgggtgg gccggttctt
      421 cagaaagatt tcacagcgtc ggatcggaag cgcgatcccc ggcttctccc cggctaccgc
      481 caatcacccc gtcggtgaaa cgcgccaatg ggtaaagcgc cgccgcacgg ttctcctccg
      541 gcgaaagcgg ggcactacgt aaaaatacgc gaaaatagtc atccagaatg aaacccgccc
      601 cgggatggta gtcgcgctca tcctttttat gccttccgaa cccgaccctt ctgccgccgc
      661 gcctctctca tttttcccgg ccagcacgtc gcctcgtcct tattcaacgg aagggcgtgc
      721 cgcggaagaa gcgatcgccg ccgccggccg cgtggtcgag cagatcctcg gtgacgcccc
      781 gctcctcgct ttgctcgccg cgcgaggcat cggctcgacc gaagtgcagc gcggcgccgc
      841 tctccatatt ctcgcgcaac aagcctgcga aacctgccaa ggccaccttg gcgcactgga
      901 tgccgcgcac gaggcgcgcg cgctcgcgct ggcctccgcg cgcgaggatt acctcgattt
      961 ccgtcgcgcc gtgaaagccg cctgcgcgca cgacagcgtc cgcgatcctc tcgaagcgag
     1021 cgtaaaccca caccagagtc tccgcgcttt cgtcgttcaa gccaccgcct cctacctcgc
     1081 cgcgctgaaa acgctccctc cggccctcat cgcgcaacac ggcttcaatc tcactcgcac
     1141 caacgccgcc cttgcccgtg tgcaacggct cgtggttctc gacactttgt ttaacacccg
     1201 ggcc
//
LOCUS       DASPLT010001247         1613 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_280736, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001247 DASPLT010000000
VERSION     DASPLT010001247.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1613)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1613)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1613
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_280736"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1531
                     /locus_tag="VEA63_12750"
     CDS             <1..1531
                     /locus_tag="VEA63_12750"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase"
                     /protein_id="HYD84923.1"
                     /translation="ASLQDYRAKYNIASLGENQSVVLQKVGTVGTALVRAQMEQVEMR
                     SVLDKIEEYRADNRDLLEIPQIASYGQVSGLRGRLAELKAQRLLLSEKYLRLHPRMVQ
                     NELETNETARLLDEAITMSIANLETRLRIAMQHESRLRQELQEAENMAHQLDKTSVDY
                     RFLEQDAATKRATYSRIVDRLNEASVASQMENTNIKIFDPAYVPVSPVGDDALELGLK
                     AGFIGFGLLVFVPLGFGFFDTRIKTVSHVEEALGEVLLGAVKTIDGLGEIERAHVYRL
                     HKDDGLTESYRGIYSSIDIHSTEGFPKAIVSTSSMPGDGKSLTASNLAAVFAAHGRRT
                     LLVDCDLRRPVLHRYFGVDVTNGWIQCLTSAALAANPPPEPPTIAFTENLDLLPSGGI
                     AKNPTETLEKFVTSGMLKRLLTRYDLVILDTPPVAVFPDALLLSRYCKELIYVCKYGT
                     VRLHNVRKTLHKLHETGIKVLGLVINQMPESRFRTSGYQGYGAYGQEYYQAYAKTSPA
                     H"
BASE COUNT          325 a          549 c          458 g          281 t
ORIGIN      
        1 ggcctcgctg caggattacc gcgccaaata caacatcgcc tcgctcggtg agaaccagag
       61 cgttgtgctg caaaaggtcg gcaccgtcgg caccgcgctc gttcgcgccc agatggagca
      121 ggtcgaaatg cgttcggtgc tcgacaagat cgaggagtat cgcgccgaca accgcgatct
      181 tctcgaaatc ccgcagattg cttcctacgg ccaggtcagc ggcctgcgag gacgtctcgc
      241 cgaactcaag gcgcagcgtc tgcttctctc cgaaaaatac ctgcggctgc atccgcgcat
      301 ggtgcagaac gaactcgaga ccaacgagac cgcgcgactc ctcgacgagg cgatcacgat
      361 gtcgattgca aatctggaaa cgcgcctccg catcgcgatg caacacgagt cgcgcctccg
      421 ccaggaactc caggaagccg agaacatggc gcaccaactc gacaagacgt ccgtcgatta
      481 ccgtttcctc gaacaggacg ccgcgaccaa gcgcgccacc tactcgcgca tcgtcgaccg
      541 actcaacgag gcgagcgtcg ccagccagat ggagaacacc aatatcaaga tcttcgatcc
      601 cgcttacgtg ccggtcagcc cggtcggcga cgacgcgctc gaactcggct tgaaggccgg
      661 cttcatcggc ttcggcctgc tcgttttcgt tccgctcggc ttcggctttt tcgacacgcg
      721 catcaaaacc gtgtcgcacg tcgaagaagc gctcggcgaa gttctccttg gcgcggtcaa
      781 gaccatcgac ggtttgggcg aaatcgagcg cgcgcacgtg tatcgcctgc acaaggatga
      841 cggtcttacg gaaagttatc gaggcatcta cagctcgatc gacatccact ccacggaagg
      901 ttttccgaag gccatcgtca gcaccagcag catgcccggt gacggcaaga gcctcaccgc
      961 gagcaatctc gcggcggtct tcgcggcgca cggtcgtcgc acgttgctcg tcgattgcga
     1021 cctgcgccgt ccggtcttgc accgctactt cggcgtcgat gtcacgaacg gctggataca
     1081 gtgcctcacg tccgccgccc tcgccgcgaa cccgccgccc gagccgccca cgatcgcgtt
     1141 caccgaaaat ctcgatctcc tgccctccgg cggcatcgca aagaacccga ccgagacgct
     1201 cgagaagttc gtcacctccg gcatgctcaa gcgactgctc acgcgctacg atctcgtgat
     1261 cttggacacg ccgccggtgg cggttttccc cgacgcgctg ctactcagcc gctactgcaa
     1321 ggagctcatt tacgtctgca aatacggcac cgtgcgcctg cacaatgtgc gcaagacact
     1381 gcataagctg cacgagaccg gcatcaaggt gctcggcctc gtcatcaacc agatgccgga
     1441 gtcgcgcttc cgcacgagcg gctatcaggg ctacggcgcg tatgggcagg agtattacca
     1501 ggcctacgcg aagacgagcc ccgcgcattg atcgtcggcc ttcctcgcag tgacgcagct
     1561 cgagatacat cgcgcgcccc ggctcgcggg agcgcttcgc aatgtcgcag gcg
//
LOCUS       DASPLT010001248         1616 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_219214, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001248 DASPLT010000000
VERSION     DASPLT010001248.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1616)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1616)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1616
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_219214"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1601)
                     /locus_tag="VEA63_12755"
     CDS             complement(<1..1601)
                     /locus_tag="VEA63_12755"
                     /inference="COORDINATES: protein motif:HMM:NF012356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltotransferase domain-containing protein"
                     /protein_id="HYD84924.1"
                     /translation="MDHFESWQRDFEKKLEAGQDMSVDLLTGAELARAATEKARGEDR
                     AVLERWAEEIGERGGAMEKRIARARDVAAHALMRRYPDRTLATRFERELVVWAEPVLA
                     RYGAWYELFPRSCGSDGKRHGTLRDVESQLPRIAAMGFDVLYLPPIHPIGRAFRKGKN
                     NSPKAGPGDPGSPWAIGSEEGGHKAIHPELGTLEDFRRLLAKAKERGIEVALDIAFQC
                     SPDHPYVREHPEWFKRRPDGTIQYAENPPKKYQDIYPFDFETAAWRELWQEARSVIEF
                     WIQQGVTIYRVDNPHTKAFPFWEWCIGELKRERPELIFLSEAFTRPKVKYNLAKLGFT
                     QSYNYFPWRNTSAELREYFTELTRTQVKEFFRPNLWPNTPDILPQSLQFGGRPAFMSR
                     FVLAATLGASYGIYGPAYELGVNAPFKAGGEEYLDSEKYEIKQWRLDAPESLEPLITK
                     VNAIRRENPALHTNERLAFHGMTNPQLIAYSKRTADAENIILTIVNLDPHHAQEGFTA
                     LDLAELGVDSKDTFQVHDLLTGSRYL"
BASE COUNT          305 a          589 c          392 g          330 t
ORIGIN      
        1 agataacgcg aacccgtcag caaatcgtgc acttgaaacg tgtccttgga atcgacgccg
       61 agttccgcga gatcgagcgc tgtgaaacct tcctgcgcat gatgcgggtc gagattcacg
      121 atcgtgagaa tgatgttctc cgcatccgcc gtgcgcttgc tgtaagcaat caactgcgga
      181 ttcgtcattc cgtgaaacgc cagcctctcg ttggtgtgca gcgctggatt ctcgcgacgg
      241 atcgcattca ccttcgtaat cagcggctcc aggctctccg gcgcatcgag ccgccattgc
      301 ttgatctcgt atttctccga atcgagatac tcctcgccgc ccgccttgaa cggcgcgttc
      361 acgcccagct cataagccgg cccgtagatt ccgtaactcg cgccgagcgt cgccgccaac
      421 acgaaccgcg acatgaacgc cggccgcccg ccgaactgta gcgactgcgg caaaatgtcc
      481 ggcgtgttcg gccaaagatt cggccggaaa aactccttca cctgcgtccg cgtcagttcg
      541 gtgaaatact cgcgcagctc cgcactcgtg ttccgccacg ggaaataatt gtaggactgg
      601 gtaaacccca gcttcgccag gttgtatttt accttcggcc gcgtgaacgc ctccgacaaa
      661 aagatcagct ccggccgctc ccgcttcagc tcgccgatgc accactccca aaaggggaac
      721 gctttcgtgt gcgggttatc gacccggtag atcgtcacgc cctgctgaat ccaaaattca
      781 attacgctcc tcgcttcctg ccacagctcg cgccacgccg ccgtctcgaa atcgaacgga
      841 taaatatcct gatacttctt cggcggattc tccgcgtatt ggatcgtccc gtccggccgt
      901 cgtttgaacc actccggatg ctcccgcaca tacggatgat ccggcgaaca ttgaaatgcg
      961 atgtccagcg ccacctcgat cccgcgctcc ttcgcctttg ccaatagccg ccgaaaatcc
     1021 tccagcgtgc ccaactccgg atgaatcgcc ttgtgaccgc cttcctcgct tccgattgcc
     1081 cacggactcc ccggatcgcc cggtcccgcc ttcggtgaat tgtttttccc tttccgaaac
     1141 gcccgcccga tcggatgaat cggcggcaga tacaacacgt cgaatcccat cgccgcgatc
     1201 cgcggcaact gcgactccac gtcccgcagc gtcccgtgtc gctttccatc cgaaccacac
     1261 gaccgcggaa ataactcata ccacgcgccg taacgcgcga gcaccggctc cgcccatacc
     1321 accagctctc gctcgaatcg cgtcgcgagc gtccgatccg gataacgccg catcaacgca
     1381 tgcgccgcca catcgcgcgc tcgcgcgatg cgtttctcca tcgcgccacc ccgctcaccg
     1441 atttcctccg cccaccgctc cagcaccgcg cgatcctcac cgcgcgcttt ctctgtcgcc
     1501 gctcgcgcga gttccgcgcc cgtcagcagg tcgacactca tgtcctgtcc tgcctccaac
     1561 ttcttctcga aatcgcgctg ccacgactcg aagtgatcca cccagccttc gatcgt
//
LOCUS       DASPLT010001249         1816 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_95953, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001249 DASPLT010000000
VERSION     DASPLT010001249.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1816)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1816)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1816
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_95953"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            154..1026
                     /locus_tag="VEA63_12760"
     CDS             154..1026
                     /locus_tag="VEA63_12760"
                     /inference="COORDINATES: protein motif:HMM:NF024777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamG-like jellyroll fold domain-containing
                     protein"
                     /protein_id="HYD84925.1"
                     /translation="MTRRYFQGFTYDQVTTGWSFDSTNDWSVCAWVYIASNGSGGGDV
                     FFQDDGTYIPTNMAYLGYERNAANTGPALRFVCGDGSVRVMDRSQDCNTGQWYHMALV
                     HDASAGTVTAYVDAVQVEQATSVTLTTTFTRTEYGDFGGGNDVEVAQAKAWNGYALSA
                     ADIADEMAYWTPQHGTGFVVAWWQFDNASQLTDSSGNGHTIGNVASGGGSPGPGLQTV
                     PGQLNPSPTVLPVAASGGAVGGGTAAVSYKARVQATGNAVASGTAWVSTNVAPAGGSA
                     SSFGGRSRKRRNRR"
     gene            1365..1592
                     /locus_tag="VEA63_12765"
     CDS             1365..1592
                     /locus_tag="VEA63_12765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD84926.1"
                     /translation="MKPSMSDKALQTAIVVFGAMIVGLAEAPGIEALIPQPWLHIVQL
                     VAAAVGASQLIKRRGDYAPSEVTLVERKSVT"
     gene            1589..>1816
                     /locus_tag="VEA63_12770"
     CDS             1589..>1816
                     /locus_tag="VEA63_12770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD84927.1"
                     /translation="MISREELELLISGAVQQAALAIESAHGRTLTRTELGQLNASISV
                     ALRMVAERCAAAKVPPPPPASRHPARFFNPVA"
BASE COUNT          339 a          565 c          614 g          298 t
ORIGIN      
        1 gagcgcgtca cgcctcagaa gatccgcgtt caagcgtggg gcggcgtgtt cgagcgtccg
       61 ctgccggcgc agcgcgagct cgacgagcag atcgcagagt ccgaagcgct cgccgctcgg
      121 atcgacaccg accccgagca ctaaaggtgt gagatgaccc gccgctattt ccaaggcttc
      181 acctacgacc aggtcacgac cgggtggtcg tttgattcta caaacgattg gtctgtgtgc
      241 gcttgggtct acatcgcatc gaacggtagc ggcggcggtg acgtcttctt ccaggatgac
      301 ggcacgtaca tccccaccaa catggcgtac ctgggctacg agcgcaacgc agctaacacc
      361 gggcctgcac tgcggttcgt ttgtggtgac ggctctgtgc gcgtcatgga tcgctcgcag
      421 gactgcaaca ccggccagtg gtaccacatg gcccttgtgc acgacgcgtc tgcgggcact
      481 gtcactgctt atgtagacgc tgtgcaggtc gagcaagcca cgtcagttac gcttacgact
      541 accttcacgc gcacggagta tggagacttc ggcggcggca acgatgtaga ggtcgcgcag
      601 gcgaaggcgt ggaacggata cgcgctcagc gcggccgata ttgccgatga aatggcgtat
      661 tggacgccac agcacgggac cgggttcgtt gtcgcttggt ggcagttcga caacgcaagc
      721 cagctaactg acagttcggg caatgggcac acgatcggca acgtcgctag cggcggcggc
      781 agtcctggcc caggcttaca gaccgtcccc ggacagctga acccgtctcc taccgtgctg
      841 ccggtcgcag ccagcggcgg cgcagtgggc ggaggcactg ccgccgtcag ctacaaggcc
      901 cgcgtgcaag ccactggcaa cgctgtcgcg agtggcaccg cttgggtgag cacaaacgtc
      961 gcgcccgcgg gtggtagcgc ctcgtcgttc ggcgggcgct cccgaaagcg acgcaaccgt
     1021 cgctagcggc ggcgcctaac agtgctaaac tgttaggcag gacacaacaa gcggcgccga
     1081 tgtatgaccg agcacccgtt tgcgaagcac gcctcggtcg cggttaccgt gatcggcgca
     1141 gtcgcggccg tgtggactcg gttcatcgtc atcgaggagc ggcaagcgaa tcagctgctg
     1201 cgaatcgctg agctaagcgc cgaagtgaag gcgctacggg cgcacatcga atcagaggag
     1261 cgcgccacgc gcgagcgttt ccacgcgagc gatctgcggc tgagtggtct ggaagatcac
     1321 gtcagtacgt ttgtggtcac gactggacgt aggaggcgaa cgcgatgaaa ccgagcatga
     1381 gcgacaaggc actgcagaca gcgatcgttg ttttcggcgc gatgattgtg ggcctcgccg
     1441 aggcgcctgg aatcgaagcg ctcatcccgc agccgtggtt gcacatcgtg cagctggtcg
     1501 cggcggcggt gggagcctcg cagctgatca agcggcgcgg cgactacgcg ccgagcgagg
     1561 tcacgctcgt ggagcgaaag agcgtcacgt gatcagccgg gaggagctcg agctgctcat
     1621 ctcgggcgcg gtgcagcagg ccgcgctcgc gatcgagtcg gcgcacgggc gcacgctcac
     1681 gcgcaccgag ctcgggcagc tcaacgcctc gatcagcgtc gcgctgcgca tggtggccga
     1741 acggtgtgcg gcggcgaagg tcccccctcc gccgccagca agccggcacc ccgccagatt
     1801 cttcaaccct gtggca
//
LOCUS       DASPLT010001250         1402 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_15697, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001250 DASPLT010000000
VERSION     DASPLT010001250.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1402)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1402)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1402
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_15697"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..466
                     /locus_tag="VEA63_12775"
     CDS             <1..466
                     /locus_tag="VEA63_12775"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HYD84928.1"
                     /translation="LWIASAGALALLAIETAGEHALGIAAEQSTMTWLFALYTLTAAI
                     IEEIIFRGYLVIEGRGTAARWVGILAASAVFALVHPFLWKWDDAGFALTFGAKGAFST
                     AIVFATSVWLYVARFARWNPTQSLLPCIVAHAAKNAGVIVVKAASGFMGGLW"
     gene            complement(696..>1402)
                     /locus_tag="VEA63_12780"
     CDS             complement(696..>1402)
                     /locus_tag="VEA63_12780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HYD84929.1"
                     /translation="LQGARASVTADPGQVTRISYLDLSNDIVQVEFSGAGRLSIELEN
                     ASGPVAALNYNQPGVTYMKGHAGIVITGANETTNVSIFTVGTLTALNQSLFRSDVSYD
                     GVADVGYLAILSSNGKFGSIRTANANFVGASGVVGICAPGVEFVGSVFVHDINASDKG
                     NPMLLLGGAGDVQINGGALWQANSRPVQVGGFARLKFVDGMTSRGTLLPAQISQSRLE
                     QNGADVSTQVVVNPGL"
BASE COUNT          264 a          477 c          403 g          258 t
ORIGIN      
        1 gctctggatc gccagcgccg gcgccctcgc tctgctggcg atcgaaaccg cgggcgaaca
       61 cgcgctcggt atcgcggccg agcaatcgac gatgacctgg ctgttcgccc tctacacgct
      121 cacggcagcg atcatcgagg agatcatctt ccgcggttat ctggtgattg aaggccgtgg
      181 caccgcggcg cgctgggtgg gaattttagc cgcgtcggcg gtgttcgcgc tcgtgcatcc
      241 gtttctctgg aagtgggacg atgcgggctt cgcgctgaca ttcggcgcga aaggcgcctt
      301 tagcaccgcc atcgtgttcg cgacctcggt gtggctctat gtggcgcgtt tcgcacgctg
      361 gaacccgacg cagtctctcc tcccctgcat cgtcgctcac gccgccaaaa acgccggcgt
      421 aatcgtcgtg aaagccgcca gcggcttcat gggcggcctc tggtaagagg cccacactcg
      481 cggcgctttc aacaaagtga tggtcactgg cgtaggacaa ctccgcatca tccgcgaccg
      541 accacgatga agcgccattc acccgtagcg gcaggcgtcc ctgcctgcct gcaatccgtc
      601 gattgcgcgc tcgcgcgcaa cccgtggcgc gacgagcgcg ccacctacac ctcagctaaa
      661 aagcggtaga agtcgccgcg ctcgccaaac tcaacttaga gccccgggtt caccaccacc
      721 tgcgtagaca cgtcggcgcc attttgctcg agacgcgatt gactgatctg cgccggcagc
      781 aacgtcccgc gcgacgtcat gccatcgacg aatttcaacc gtgcgaagcc acccacctgc
      841 accggccggc tattcgcctg ccaaagcgcg ccgccgttga tctgcacgtc gccggcgccg
      901 ccaagcagca acatgggatt acctttgtca gaagcattga tgtcgtgaac gaataccgaa
      961 ccgacgaact ccacgcccgg cgcgcaaatc cccacgacgc cgctcgcgcc cacgaaattc
     1021 gcgttcgccg tgcgaatgct gccgaatttt ccgttgctgc tcaggatcgc gagataaccg
     1081 acatcggcca cgccatcgta gctcacgtcg ctgcggaaca acgactggtt caacgccgtc
     1141 agcgtgccga cagtaaaaat agaaacgttg gtcgtttcgt tcgcgcccgt gatcacgatg
     1201 cccgcgtgac ccttcatgta ggtcacgcca ggttggttgt agttgagcgc cgcgactgga
     1261 cctgacgcat tctccagctc gatcgacaaa cggcccgcgc cggagaactc cacctgcacg
     1321 atgtcattgc tcaaatcgag atacgagatg cgcgtcacct ggcctggatc ggccgtgacg
     1381 ctcgcgcgcg cgccttggag ca
//
LOCUS       DASPLT010001251         1667 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_157918, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001251 DASPLT010000000
VERSION     DASPLT010001251.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1667)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1667)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1667
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_157918"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..801
                     /locus_tag="VEA63_12785"
     CDS             <1..801
                     /locus_tag="VEA63_12785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HYD84930.1"
                     /translation="AQKLCGTFNSTGATSCFLDATQALHGDLGLCAEGDLAILLSNSG
                     QTEEILRLVPILKRLGVKLVALTGQRQSDLAQNADHRLFYRVRREACPLALAPTASTT
                     AALALGDALAMVLLEARGLTRDDFARYHPAGNLGALLLKVRDIMRTGDRLPVARETVS
                     TQDAILAMTRAKSGSIALVHAKSGKLTGILTDGDFRRSALTGPDFLQKPVSNFMTRHP
                     KVIAENALGVDALRLFQEHKIDDLIVINAQGRPVGLVDGQDLPKLKIV"
     gene            798..>1667
                     /locus_tag="VEA63_12790"
     CDS             798..>1667
                     /locus_tag="VEA63_12790"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HYD84931.1"
                     /translation="MKRPVRIGLIGMGGFAAAHHGTIARLEERGEAKLVCTCDPKAAL
                     FAQQQEVWKFAARGVQVFEDYRAMLERCHRELDLVVIPTPIQLHAEMHAAAAALGLPS
                     YLEKPATLDYAQLEQMIATDARLPKASLVGFNFIIEKPRLALKERILSGEFGAVQGAT
                     LTGLWPRPTSYFQRNEWAGRLMIGDHIVLDSCLGNALAHFVHNLLFWAGHGDLFSWAK
                     LAAVRAELYRAHRIEGADTFFVETDTTAGVTLRFALSHACSGSSSQTEVVICEKATLR
                     YIVGQFAEVRWLDG"
BASE COUNT          307 a          574 c          499 g          287 t
ORIGIN      
        1 gcgcaaaagc tctgcggcac cttcaacagc acgggcgcaa cgtcttgctt cctcgacgcg
       61 acgcaggcgc ttcacggcga tctcgggctt tgcgccgaag gcgacctcgc catcctgctg
      121 agcaacagcg ggcagaccga ggaaatcctc cgcctcgtgc cgatcctgaa gcgattgggc
      181 gtgaagctcg tcgcgctcac cggccagcgc cagtccgacc tggctcaaaa cgcggatcac
      241 cgattgttct atcgcgttcg acgcgaagcg tgtccgctcg cactcgcgcc gaccgcgagc
      301 accaccgccg cgctcgcgct cggcgacgcc ctcgcgatgg tgctgctcga agcgcgcggg
      361 ctcacacgcg acgatttcgc gcgttatcac ccggccggga atctcggtgc cttgttgctc
      421 aaagtccgcg acatcatgcg caccggcgat cggcttcccg tcgcgcgcga aacggtgagc
      481 acgcaggatg cgatcctcgc gatgacccgc gcgaaatccg gcagcatcgc gctcgtgcac
      541 gcgaagagcg gcaagttgac cggtattttg acggacggcg attttcgccg tagcgctctc
      601 accggacccg atttcttgca gaagcccgta tccaatttca tgacgcgcca ccccaaggta
      661 atcgccgaga acgcgctcgg cgtcgacgca ctgcgcctgt ttcaggagca caaaatcgac
      721 gacctcatcg tcatcaacgc gcaaggacgg cccgtcggcc tcgtcgacgg ccaggatcta
      781 cctaaattga aaatcgtgtg aagagacctg ttcgcatcgg tctcatcgga atgggtggct
      841 tcgccgccgc ccaccacggc acgatcgccc ggctcgaaga gcgcggcgag gcgaagctcg
      901 tgtgcacctg cgatcccaaa gccgctttgt ttgcacagca acaagaggtg tggaagttcg
      961 ccgcccgcgg cgtgcaggtg ttcgaggatt atcgcgcgat gctcgaacgc tgccatcgcg
     1021 aactcgatct cgtcgtcatc ccgacgccga tccaactgca cgcggaaatg cacgccgccg
     1081 ccgccgcgct cggcctgccg agctacctcg aaaaacccgc cacactcgat tacgcgcagc
     1141 tcgagcaaat gatcgcgacg gatgcgcgcc tgccgaaggc ttcgctcgtc ggcttcaatt
     1201 tcatcatcga aaagccgcgg ctcgcattga aggagcgcat tctcagcggc gagtttggcg
     1261 cggtgcaggg cgcgacgctc accggtctct ggccgcggcc gaccagctac tttcaacgca
     1321 acgaatgggc cggccgcctg atgatcggcg atcacatcgt gctcgactcg tgcctcggca
     1381 acgcgctcgc gcacttcgtg cacaacctgt tgttctgggc cgggcacggc gacctgttca
     1441 gctgggcgaa gctcgcggcg gtgcgcgccg aactttaccg cgcgcaccgc atcgaaggcg
     1501 ccgatacatt tttcgtcgag accgacacga ccgccggcgt cacgctgcgc ttcgcacttt
     1561 cgcacgcctg ctccggcagc agctcgcaaa ccgaagtggt gatctgcgaa aaagccacgc
     1621 tgcgctacat cgtggggcag ttcgccgaag tgcgctggct cgacggc
//
LOCUS       DASPLT010001252         1119 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_302197, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001252 DASPLT010000000
VERSION     DASPLT010001252.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1119)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1119)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1119
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_302197"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..301)
                     /locus_tag="VEA63_12795"
     CDS             complement(<1..301)
                     /locus_tag="VEA63_12795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HYD84932.1"
                     /translation="MPTFSYTAIEAATGRERRGTENGMSAAHVASALKARGLAPTSLA
                     PVAAMGGAARGEKRTAVKPVMARASGAKRGAKRMVAFGRAASVKEIALFTRQLSTL"
     gene            complement(468..>1119)
                     /locus_tag="VEA63_12800"
     CDS             complement(468..>1119)
                     /locus_tag="VEA63_12800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007358652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HYD84933.1"
                     /translation="TAEDPVEYEMEGMMQVPVNPLAGLTFASALRSFLRQDPDVIMVG
                     EIRDLETAQVAIQASLTGHLVLTTLHTNDAPGAVTRLIDMGVEPFLIASTLEGVLAQR
                     LLRRVCVKCRTEYTPSAEWLAQLGVDGEAGRREGKLFYRGRGCAHCAQTGYRGRVGIF
                     EWLRMTEALREQVAVKASLLRLRELGLAQGMRPLREAALRAMFAGETTIEEVVKYT"
BASE COUNT          193 a          465 c          273 g          188 t
ORIGIN      
        1 ccaacgtcga aagctgccgc gtgaacagcg caatctcctt cacgctcgcc gcccgcccaa
       61 acgccaccat gcgtttcgcg ccccgcttcg ctccgctcgc gcgcgccatc accggcttca
      121 ccgccgtccg cttctcccct cgcgctgcgc cacccatcgc cgctaccggc gccaacgacg
      181 tcggcgcaag cccgcgcgct ttcagtgccg acgccacgtg cgccgcgctc atcccattct
      241 ccgtcccgcg ccgctcgcgc cccgtcgctg cctcgatcgc cgtataagaa aaggtcggca
      301 taacgatcaa ttcgccatcc gctccacctt caacccctcg cgttgcgcgt cgctcgctcc
      361 atgaggagcg gagcacttcc gccacgcatt cgctcctgcg cgccgcgcat agcccgctcg
      421 gtgaggagcg ggacacttgc gctccgtgaa cattcctcca cccgccgtca tgtgtatttc
      481 acgacctcct cgatcgtcgt ctcgcccgcg aacatcgctc gcaacgccgc ctcacgcagc
      541 ggccgcatgc cttgcgcgag cccgagttcg cgcaatcgca gcaacgacgc tttgaccgcc
      601 acttgctcgc gcagcgcctc cgtcattcgc aaccattcga aaattcccac gcggccgcga
      661 taacccgtct gcgcacaatg cgcgcaaccg cgaccgcgat agaacaactt cccctcacgt
      721 cgaccggcct ccccatcgac gccaagctga gccagccact ccgccgatgg cgtgtattca
      781 gtccggcact tcacacacac ccgccgcaac agccgctgcg cgagcacgcc ttcgagcgtc
      841 gacgcaatca agaacggctc cacccccatg tcgatcaacc gcgtcaccgc gccgggtgca
      901 tcgtttgtat gcaaggtcgt cagcaccagg tgccccgtga gcgacgcctg gatcgccacc
      961 tgcgccgtct ccaaatcccg aatctcgccc accatgatga cgtccggatc ctgccgcaga
     1021 aacgatcgca gcgccgatgc aaacgtcagc cccgcgagcg gattcaccgg cacttgcatc
     1081 atgccttcca tctcgtattc caccggatcc tccgcggtc
//
LOCUS       DASPLT010001253         1483 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_280813, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001253 DASPLT010000000
VERSION     DASPLT010001253.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1483)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1483)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1483
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_280813"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..482
                     /locus_tag="VEA63_12805"
     CDS             <1..482
                     /locus_tag="VEA63_12805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HYD84934.1"
                     /translation="RIPFIWELNVHGYDYACLERSRPQDPEYYRHNSSDLGVVEHFLG
                     APAPALSFDLHTLRETDLPHAIHLSRTIVNAGRLDGFAVFFRAHVDDDLTLSSGPLDA
                     NRAPHWGFRILRADAENFAAGDTIELTLTVGQWTEPDTWRWTHQHTPAGPSPSPAAA"
     gene            complement(549..1307)
                     /locus_tag="VEA63_12810"
     CDS             complement(549..1307)
                     /locus_tag="VEA63_12810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD84935.1"
                     /translation="MRVSKGETEAHRELKRLALGWAQANGFAVCACEVRVPKSGCRAD
                     VAAIARGADARTAVFECKQARGDLLKDARREEEAREKVAEFAERLKKLEELIGGHRPD
                     LRKGEALFPEFDAWDFSGIEHETHRRVLAELAMWQERAVCGTKFAKLFRWRAADYFYL
                     VSEEGIFAEAEIPAGWGLLVRRGEELVLVRKPVWAEASKEAREALGGCIGISATRAVN
                     REMGVRLPAPPWREEEKAALRMSTVPRANSPSDA"
BASE COUNT          259 a          638 c          315 g          271 t
ORIGIN      
        1 gccgcattcc cttcatctgg gaactcaacg ttcacggcta cgactacgcg tgcctcgagc
       61 gaagccgccc gcaagatccc gagtattacc gccataacag cagcgacctc ggcgtcgtgg
      121 aacatttcct cggcgcaccc gcgcccgcgc tctccttcga tctccacacg cttcgcgaga
      181 ccgatctccc gcacgcgatt cacctttcgc gcaccatcgt caacgccggc cgactcgacg
      241 gcttcgccgt cttcttccgc gcccatgtcg acgacgatct cacgctcagt tccggcccgc
      301 tcgacgcgaa ccgcgcgccg cattggggct ttcgcatcct ccgcgccgac gccgagaatt
      361 tcgccgcagg cgacaccatc gagctcacgc tcaccgttgg ccaatggacg gaacccgaca
      421 cctggcgctg gacacaccag cacacgcccg ccggcccgag tccctctccc gccgccgcct
      481 gacacacggc tccgctcccg aacgaaccgg cacacggtag ctgcacgtgt ctccacgtgc
      541 cctcggagtt aagcgtccga aggtgaattc gcgcgcggaa cggtgctcat gcgcagtgcc
      601 gccttctcct cttcccgcca cggcggagcc ggcaacctca cgcccatctc gcggttcacc
      661 gcccgcgtag ccgaaatccc gatacaccca cccaacgctt cccgcgcttc cttgctcgcc
      721 tccgcccaaa ccggtttccg cacgagcacc aattcctctc ctcgccgcac cagcaagccc
      781 catcctgccg gtatctccgc ttccgcgaaa atcccctcct cgctcaccaa ataaaaataa
      841 tccgccgccc gccagcgaaa cagcttcgcg aacttcgtcc cgcagaccgc gcgctcctgc
      901 cacatcgcca actccgccaa cacgcgtcga tgcgtctcat gctcgatgcc cgaaaaatcc
      961 cacgcgtcga actccggaaa cagcgcctcg cctttccgca aatccggtcg atgcccgccg
     1021 atcagttcct ccagcttctt caaccgttcc gcgaactccg ccactttctc ccgcgcctcc
     1081 tcctcgcgcc gcgcatcctt caacaaatcc ccgcgcgcct gcttgcactc gaacaccgct
     1141 gtccgcgcgt ccgctcctcg cgcgatcgcc gcgacgtccg cccgacaccc gctcttcggc
     1201 acgcgcacct cacacgcgca caccgcgaat ccgttcgctt gcgcccagcc cagcgccagc
     1261 cgcttcaact cccgatgcgc ctccgtctct cctttgctca cacgcatcac ttcaaacccc
     1321 cactttcctt ctgaccacgt aaatctgccc gcttcttatc tctctgcctc catgcctcgg
     1381 tggttcaaaa tcctatcttc aaccgcacat caaaaaatcc cgtccttcca catacctccg
     1441 ccgcaccatt tcctcgcgat taaactcccg cgctcctcgc gtc
//
LOCUS       DASPLT010001254         1346 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_260564, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001254 DASPLT010000000
VERSION     DASPLT010001254.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1346)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1346)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1346
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_260564"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..638)
                     /locus_tag="VEA63_12815"
     CDS             complement(<1..638)
                     /locus_tag="VEA63_12815"
                     /inference="COORDINATES: protein motif:HMM:NF024799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HYD84936.1"
                     /translation="MTSRPLPLLISVAVTFLALAPFASTASASKEKPVIGLSLDTLKE
                     ERWQQDRDTFTAAAEKLGARVIVQSANSDDTRQVRDVESLISRGVDVLVIVPHNGAAM
                     TRAVKSANEAKIPVIAYDRLILNVDIDYYLTFDNVKVGEAQATYAVARLPKDRPARIV
                     RIYGAPTDNNAKLFKQGQDNILQPLIDSGKIVVLHEDWALDWKPDVAKRIMNA"
     gene            762..>1346
                     /gene="purN"
                     /locus_tag="VEA63_12820"
     CDS             762..>1346
                     /gene="purN"
                     /locus_tag="VEA63_12820"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007361798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HYD84937.1"
                     /translation="MKIGGPMRVVILGSGRGSNAEAILEAEKAGRLGRARVVQIFSDR
                     ADAGILALGPKFGVPAAFLDAAPFKTKLDGDGEARYIAAIESCRPDLVVLAGFMRVLK
                     PGFLKAFEERIINLHPSLLPSFPGLDGIGQAWRRGVKVTGCTVHRVTSEVDGGPIIDQ
                     AAVRIETTDTLGTLTAKVHAAEHALLPAVIARLSE"
BASE COUNT          232 a          310 c          529 g          275 t
ORIGIN      
        1 gcgttcatga tgcgtttggc gacgtcaggt ttccagtcga gcgcccaatc ctcgtgcagg
       61 acgacaattt tgccggaatc gatgagcggc tggaggatgt tgtcctggcc ctgtttgaag
      121 agtttggcgt tgttgtcggt gggcgcgccg tagatgcgga cgatgcgcgc gggccgatct
      181 ttcgggaggc gggcgacggc gtaggtggct tgggcttcgc cgacctttac gttgtcgaag
      241 gtgagatagt aatcgatgtc gacgttgagg atgaggcggt cgtaggcgat gacggggatc
      301 ttggcttcgt tcgcggattt gacggcgcga gtcatggcgg cgccgttgtg tgggacgatg
      361 acgagcacgt cgacgccgcg gctgatgagg gattcaacgt cgcggacctg gcgggtgtca
      421 tcgctgttgg cggactgaac gatgacccgt gcgccgagtt tttcggcggc ggcggtgaag
      481 gtgtcgcggt cttgttgcca acgttcttct ttcaacgtgt ccaacgagag gccgatgact
      541 ggtttttcct tggaagctga ggccgtggaa gcgaagggcg ccagagcaag aaaggtgacc
      601 gcaacggaga taagcagggg aagggggcgg gatgtcatgg ggagcgcaag gagagcaggg
      661 attgcgagat ttacaatcgc gcggaggagg aacgatcggc ggcgatgcgt tcgcgagaga
      721 gcgggacgcg cacgaatgtc atcgccagat gcggcggcgc cgtgaaaatc ggcggcccta
      781 tgcgcgtggt gattcttggt tcgggccgtg gctcaaatgc ggaggcgatt ctcgaggccg
      841 agaaagcggg gcgtctcggg cgggctcgcg tggtgcaaat tttctcggat cgggcggacg
      901 cggggatttt agcgcttggg ccgaagtttg gagtgccggc ggcttttctg gatgcggcgc
      961 cgttcaagac gaagttggat ggggacggcg aggcgcgcta catcgcggcc atcgagtcgt
     1021 gtcggccgga tttggtggtg ctggcgggtt tcatgcgcgt gttgaagccc gggtttttga
     1081 aggcgtttga agaacgcatc atcaatttgc acccgagctt gctgccgagt tttccgggct
     1141 tggacggaat tgggcaggcg tggcgtcgcg gggtgaaagt gacgggttgc acggtgcatc
     1201 gagtcacgag cgaggtggac ggcgggccga tcatcgatca agcggcggtg cgcatcgaaa
     1261 cgacggatac gctgggaacg ctgacggcga aggtgcacgc ggcggagcac gcgttgctgc
     1321 cggccgtgat cgcgcgattg agcgag
//
LOCUS       DASPLT010001255         1517 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_116408, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001255 DASPLT010000000
VERSION     DASPLT010001255.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1517)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1517)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1517
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_116408"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1072
                     /locus_tag="VEA63_12825"
     CDS             <1..1072
                     /locus_tag="VEA63_12825"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HYD84938.1"
                     /translation="AKPLRIAGINFDHMHMGDLLRQVHEHPTAEIVGICDEKPARMKT
                     AIANFGIPPERVFTDYRRCLETTKPDLVILCPATAHHARWTQLVAPYRTHILVEKPFA
                     ATLAQADAMIAAVAATKKHLIINWPLRWYPSHITAKRLVDEGRIGRVTEVHYYDGNRG
                     PLAHRADKVEAIPTAARKAKSWFYQESAGGGSLLDYLGYGTTLGTWFMDGRAPIEVTA
                     TVDQPKGLEVDEHAIVVARYETGLSKFETRWGTFTDPWKMQPQPKCGFVLVGTKGTIS
                     TYDYEPTIRVQDAAHPEGEVVPVDKIRPPFHNAIAYTVDCLARGKKVDGPLSPKIARI
                     GQQIVDTAVQSARLRKTVKLID"
     gene            1113..>1517
                     /locus_tag="VEA63_12830"
     CDS             1113..>1517
                     /locus_tag="VEA63_12830"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HYD84939.1"
                     /translation="MSKNYDLTAEAASEMSAPALAYEPPMPKRYRPRIGLIGCGGITQ
                     HHLRAYRDAGWDVVAFYDPVHEMAEKRRAEFYPQAHVCSSVAELLARPALDVVDIATH
                     AGVRGLLIEQAIAAGKHVLSQKPFVLELAEGRR"
BASE COUNT          307 a          554 c          406 g          250 t
ORIGIN      
        1 cgccaaaccc ctccgcatcg ccggcatcaa tttcgatcac atgcacatgg gcgatctcct
       61 ccgtcaggtg catgagcatc ccaccgccga gatcgtcggc atctgcgacg agaaaccggc
      121 gcgcatgaaa accgcgatcg ccaacttcgg catcccgccc gagcgcgtat tcacggatta
      181 ccgacggtgc ctggaaacca cgaagcccga cctcgtcatc ctctgccccg ccactgcgca
      241 ccacgcacgc tggacccaac tcgtcgcgcc gtatcgcacg catattctcg tcgagaaacc
      301 cttcgccgcc accttggctc aggccgacgc catgatcgcc gcggtcgccg caacgaagaa
      361 gcatctcatc atcaactggc cgttgcgctg gtatccttcg cacatcaccg ccaaacgact
      421 cgtggacgaa ggccgcattg gccgcgtgac cgaagtgcac tactacgacg gcaaccgcgg
      481 cccgctcgcc caccgcgccg acaaggtcga agccatcccc accgccgcgc gcaaagcgaa
      541 gagctggttc taccaagagt ccgccggcgg cggctcgctt ctggattatc tcggttatgg
      601 cacgacgctc ggcacgtggt tcatggatgg ccgcgcaccc atcgaggtaa ccgccaccgt
      661 cgatcaaccc aaggggctcg aggtggacga acacgcgatc gttgtcgcgc gttacgagac
      721 gggtctttcg aaattcgaaa cccgctgggg cacgttcacc gatccgtgga aaatgcagcc
      781 ccagccgaaa tgcggtttcg ttttggtcgg caccaaaggc accatcagca cctatgacta
      841 cgagcccacg attcgcgttc aggatgccgc tcatcccgag ggcgaagtgg tgccggtgga
      901 caaaatccgg cctccgttcc acaacgcgat cgcctacacc gtcgattgcc tcgcccgcgg
      961 caaaaaagtc gacggaccgc tctcgccgaa gatcgcgcgc atcggtcagc aaattgtgga
     1021 tacagccgtg cagagcgccc gcctgcgcaa gaccgtgaaa ctcatcgact gaacccagcg
     1081 ctcaacgtcg tcaccgcctc tcgcgttatc ccatgtccaa gaattacgat ctcaccgccg
     1141 aagccgcctc ggagatgtcc gcccccgcac tcgcctacga gcccccgatg ccgaaacggt
     1201 atcgcccacg catcggtctc attggctgcg gcggcatcac ccagcatcac ctgcgcgcct
     1261 atcgcgacgc cggctgggac gtcgtcgcgt tttacgatcc cgttcacgaa atggcggaaa
     1321 aacgtcgcgc cgaattttat ccccaagcgc acgtctgctc gagtgtcgcg gaactcctcg
     1381 ccaggcccgc gctcgacgtg gtcgacattg ccacgcacgc cggagtccgc ggcctcctaa
     1441 tcgagcaggc gatcgccgcc ggcaaacacg tcctctccca aaagcccttc gtgctcgaac
     1501 tcgccgaggg ccgccgc
//
LOCUS       DASPLT010001256         1407 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_75349, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001256 DASPLT010000000
VERSION     DASPLT010001256.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1407)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1407)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1407
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_75349"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            96..1166
                     /locus_tag="VEA63_12835"
     CDS             96..1166
                     /locus_tag="VEA63_12835"
                     /inference="COORDINATES: protein motif:HMM:NF013381.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HYD84940.1"
                     /translation="MPPPPSPAHAPATRAEVESVLRCAAPRPVPLFLPAIYEHKAWFI
                     GSTPSAISRDANLLTRAMLAEFEAIGPDALAIGVDVYNLEAEAVGCTVTFYDGADTSI
                     PGIKPGNHIVHVGDDLSNARLPNPLKDGRMPVNLAAARAVRAAVGDNYWLRGAVSGPF
                     SLAISLVGAEALFVASLDEPEWVHSVLAYAGRIIKEFSKAYIDAGVELIVFDSQASPE
                     LLSPAMYEEFVLPVTTEFTAWAATQGVRDVPLIIGGNTTPIAEIQTRTGANNLLCDFT
                     ADFDEWSAICRSAGRSMRRNISPRLIETSTPDEIYAVARREVERGRDIPGFIMGTAVI
                     PFGTPTENVLAVKRACLDAAKS"
BASE COUNT          240 a          500 c          416 g          251 t
ORIGIN      
        1 gctgaaggag aacgcatgtg attgcccgat ttgcgaagca cacgaaccca aaccgccaag
       61 acctggcaac gcatttcggg catgctggac gccgcatgcc tcccccgccc tccccggcgc
      121 acgcccccgc cacccgcgcc gaagtcgaat ccgtcctccg ttgcgccgcc ccgcgcccgg
      181 tgccgctttt cctgcccgcc atctacgagc acaaggcgtg gttcatcggc agcacgcctt
      241 ccgcgatttc ccgcgacgcc aacctcttga cgcgcgcgat gctggccgag ttcgaagcga
      301 tcggtcccga cgccctcgcg atcggtgtcg atgtctataa tctcgaggcg gaagccgtcg
      361 gttgcacggt gactttctac gacggcgccg acaccagcat tccgggcatc aaacccggca
      421 atcatattgt ccacgtcggc gacgatctct ccaacgcgag attgcccaat ccgctcaaag
      481 acggccgcat gcccgtcaac ctcgcggctg cgcgcgccgt gcgcgcggcc gtcggtgaca
      541 actactggct gcgcggcgcc gtgtccggtc cgttctccct ggcgatttcg ctcgtcggcg
      601 cggaagcgct gttcgtcgcg tcgctcgacg aacccgagtg ggtgcacagc gtcctcgctt
      661 acgcggggcg catcatcaag gaattttcca aggcctacat cgatgccggc gtcgagctca
      721 tcgtgttcga ttcccaagcg tcgcccgagc tgttgtcgcc ggcgatgtat gaggagttcg
      781 tgctgcccgt gaccaccgag ttcaccgcgt gggctgcgac gcaaggcgtg cgtgacgtgc
      841 cgttgatcat cggtggcaac accacgccga tcgcggaaat tcaaacgcgc acgggcgcca
      901 acaacctgct ctgcgatttc accgccgact tcgacgaatg gtccgcgatc tgtcgcagcg
      961 ccggccgctc gatgcgccgc aacatctcgc ctcgcctgat cgaaacctcc acccccgacg
     1021 agatctacgc ggtcgcccgc cgcgaggtgg aacgcggtcg cgacatcccc ggcttcatca
     1081 tgggcaccgc cgtgattccg tttggcacgc ccaccgaaaa cgtcctcgcg gtgaaacgcg
     1141 cctgcctcga cgccgcgaag agctgaagcg gaaagttcac ccgttgggtc cgtgtagcga
     1201 aacgcgtgag cgtttcggcc gtccgcacga gaagctcacg cttttcgcta cgaggtaaat
     1261 ttcaggcgca aaatggcgaa cgcccacggc gggcaagcgg gcgctggttg gtagccgcgc
     1321 cgtccgcggc gcgattgctg aaactcgcat tgaacattcc ggcgtcgctt tccccgcgtc
     1381 gcctgcttcg actgcgcagc gcacatg
//
LOCUS       DASPLT010001257         2161 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_302217, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001257 DASPLT010000000
VERSION     DASPLT010001257.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 2161)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2161)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2161
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_302217"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..519
                     /locus_tag="VEA63_12840"
     CDS             <1..519
                     /locus_tag="VEA63_12840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD84941.1"
                     /translation="TMGPEVDYLTRPSEDFARATVEYIDENGRPLVAETTTSWSYVGA
                     GLRLSSELLGPEYSFAYNSLDTSSKLFLSRRVTGAAGEDLVEKQNAEQGLMPVVGNEA
                     AEYGYENENRYFTRCFLEGKQPELNFHDGLEVVKLLMTAYMSAEQGRTLDWNPPGLDT
                     FIPAVAQGTWKK"
     gene            672..1928
                     /locus_tag="VEA63_12845"
     CDS             672..1928
                     /locus_tag="VEA63_12845"
                     /inference="COORDINATES: protein motif:HMM:NF015764.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015506 - nucleoside:proton symporter
                     activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015858 - nucleoside transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease"
                     /protein_id="HYD84942.1"
                     /translation="MTSSPAPTRSVAPRLFGMMLLEFFIWGAWLPLIWTYMAGLGFTG
                     TQIALVGSTFAIASIVAIFFGNQFVDRTFSAERFMAGSHLIAGLAMIGMFFTRDFVPF
                     FTLMVIHSICYVPTMSVANSLAFSHLKDATRDFGRVRVGGTVGWILASWPLYFALQDV
                     EGEALQHALSYIFIVAGGAGLLLAAFSLTLPHTPPKPATKAIEPLAWREGFRVLVRTP
                     FLLVLFVVTFIDSVIHNGYFLMAGGFLGAIGIKPEHIMPVMSLGQVAEMLTMLILGYF
                     LRRLGWKWTLTIGVLGHAVRFLVFAFFSEHIGAMIAVQLLHGICYAFFFATLYIFIDA
                     AFPHDMRTSAQGLFNLLVIGIGDLAAKWLFVPLMATMTTDAGIDYQRLFLVPSGMAIV
                     AALLLLFAFRPTAELNANARGVIKTG"
     gene            complement(1966..>2161)
                     /locus_tag="VEA63_12850"
     CDS             complement(1966..>2161)
                     /locus_tag="VEA63_12850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD84943.1"
                     /translation="GSAKGNGTLSNARLERVNVYEIGLAGSPSSAGWIRNDAVGSVIL
                     VDSKITEVRNDSTRFDVRVE"
BASE COUNT          372 a          791 c          536 g          462 t
ORIGIN      
        1 accatggggc ctgaggtcga ctatctcacg cgtccgtccg aagatttcgc tcgcgccacc
       61 gtcgaataca tcgacgaaaa cggccgcccg ctcgtcgccg aaaccaccac gtcgtggagc
      121 tacgtcggcg ccggtttgcg cctcagctcc gagttgctcg gccccgaata ttccttcgcc
      181 tacaactccc tcgacacgag ttcgaagctc ttcctcagcc gtcgcgtgac cggtgccgcc
      241 ggcgaagatc tcgtcgaaaa acaaaacgcc gaacagggcc tcatgcccgt cgtcggcaac
      301 gaagccgccg aatacggcta cgaaaacgag aaccgctact tcacgcgctg cttcctcgaa
      361 gggaaacagc cggagctcaa tttccacgac ggcctcgaag tcgtgaaact tctcatgacc
      421 gcctacatga gcgccgaaca aggccgcacg ctcgactgga atccgcccgg cctcgacacc
      481 ttcatccccg ccgtcgccca aggcacctgg aaaaaatagc gcaaaagtag ggcgggcttc
      541 agcccgccac tcgcaacgaa ggcggactaa agtccgccct acctccaatc cccacgcgga
      601 tctagcatct cagcatttca aatttcagca tctcagcatt tcagcatctc agcatttcag
      661 cttttctccc catgacctcc tcccccgctc ccacccgctc cgtcgcgccg cgcctgtttg
      721 gcatgatgct gctcgagttt ttcatctggg gcgcgtggct cccgctcatc tggacttaca
      781 tggccgggct cggtttcacc ggcacgcaaa tcgcgctcgt cggcagcacg ttcgccatcg
      841 cctccatcgt cgcgatcttc ttcggcaatc aatttgtcga tcgcaccttc tccgccgaac
      901 gcttcatggc cggcagccac ctcatcgccg gcctcgcgat gatcggcatg ttcttcacgc
      961 gcgacttcgt gccgttcttc acgctgatgg tcatccattc catctgctac gtgcccacga
     1021 tgtccgtcgc gaactcgctc gcgttttctc atctcaagga cgccacgcgc gacttcggcc
     1081 gtgtgcgcgt cggcggcacc gtcggttgga ttctcgcgtc ctggccgctc tacttcgcgc
     1141 tgcaagacgt cgaaggcgaa gccctccagc acgcgctcag ttacatcttc attgtggctg
     1201 gcggcgccgg ccttctgctc gccgccttca gcctgacgct gccccacacg ccgccgaaac
     1261 ccgccacgaa agcgattgag ccgctcgcct ggcgcgaagg ctttcgcgtc ctcgttcgca
     1321 cgcctttcct cctcgtgctg ttcgtcgtca cgttcatcga ttcggtcatt cacaacggct
     1381 acttcctcat ggcgggcggc ttcctcggcg ccatcggcat caagcccgaa cacatcatgc
     1441 ctgtgatgag tctcggccag gtcgccgaga tgctgacgat gctcatcctt ggctatttcc
     1501 tccgccggct cggttggaaa tggacgctca cgatcggcgt cctcggccac gccgtgcgct
     1561 tcctcgtctt cgcctttttc agcgaacaca tcggcgcgat gatcgccgtc caactcctcc
     1621 acggcatctg ctacgcgttc ttcttcgcca ccctctacat cttcatcgac gccgcgttcc
     1681 cgcacgacat gcgcacgagc gcccaaggcc ttttcaacct gctcgtcatc ggcatcggcg
     1741 atctcgctgc gaaatggctt ttcgttccgc tcatggccac gatgaccacc gacgccggca
     1801 tcgattacca gcgcctcttc ctcgtgccaa gcggcatggc gatcgtcgcc gcgcttctcc
     1861 tgcttttcgc gttccgtccc accgccgagc tcaatgccaa tgcccgcggc gtgatcaaaa
     1921 caggctgagc cccgatacag cgcgtctcat cgaactcccc ttcatctatt cgacgcgaac
     1981 gtcgaaccgc gtggaatcgt ttcggacttc cgtgattttg gaatcgacca atatcacgct
     2041 tccgaccgca tcgttgcgaa tccatccggc cgagctcggc gagccggcca gtccgatctc
     2101 gtaaacgttc acgcgttcga gccgcgcgtt cgacagcgtc ccgtttcctt tggcgctacc
     2161 g
//
LOCUS       DASPLT010001258         1098 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_96019, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001258 DASPLT010000000
VERSION     DASPLT010001258.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1098)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1098)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1098
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_96019"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..694
                     /locus_tag="VEA63_12855"
     CDS             <1..694
                     /locus_tag="VEA63_12855"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HYD84944.1"
                     /translation="DPQPAAGAAPTETIEVVGIARSMPLELFAKESSGTIYVPFTQGF
                     AGNVHFYVRSAIRGEPALVALREPVRRELQAAAPDVPFFRVHTFREHKDASLELWLLH
                     RTSLVATAFGAAAAFIAVIGLYGAKAYGVSRRTREIGIRLALGAEPARLRSMILREGL
                     ASGLFGIALGLLLGVALGHLLGSVIVDFNGFDPAVFGLAAFALFAAALAASWLPARQA
                     TKVSPMVALRTE"
BASE COUNT          172 a          361 c          341 g          224 t
ORIGIN      
        1 cgacccgcaa cccgccgcgg gcgccgcccc gaccgagacg attgaggttg tggggatcgc
       61 gcggtccatg ccgctggagc tcttcgcgaa agaatcctcc gggacgatct acgttccctt
      121 cacgcagggc ttcgcgggca atgttcattt ctacgttcgc tccgcgattc gcggtgaacc
      181 cgcgcttgtc gcgctgcgcg agccggtccg ccgcgagctt caagcggcgg cccccgacgt
      241 gcctttcttt cgggtgcaca ccttccgcga acacaaggac gcatcgctcg aactgtggct
      301 gctgcatcgc acgtccctgg tggcgacggc attcggcgcg gcggcggcgt tcatcgctgt
      361 aatcggactc tatggcgcaa aagcctacgg cgtttcgcgg cgcacgcgtg agatcggcat
      421 ccggctcgcg ctcggcgccg aaccggcgcg cctgcgcagc atgatcctgc gcgaaggcct
      481 cgcgtcggga ttgtttggca ttgcgctcgg cctgctgctc ggcgtcgcac tcggccattt
      541 gctcgggagc gtgatcgtgg atttcaacgg ctttgatccg gcggtgttcg gcctcgcagc
      601 gttcgcgctg ttcgccgccg cgcttgcggc ttcctggctc ccagcacggc aggcgacgaa
      661 ggtcagccca atggtggcgc tgcgaacgga gtaaaatcgc tagtcgcacg aggcctcagg
      721 ttgttggacg aggtccttgc ggcgaaatac tccgcacgat ccgcaagccc ccgcgctgat
      781 tggccgatct ggatccagcg gataggtgaa cagcttcttt gcccttcgag ttgtcccatc
      841 atacgccaac gtcaggcgcg cgacaggtct cccgttggac tacaccggcg attttaggca
      901 acgggccctg aaccgataac gcgcttgccg ctgctgatga ccccgttcgc agcgtgttcg
      961 ttcactcgaa gactaaattc gacggcgctt tggggagagg tctcggctta ttcagcatgt
     1021 tcgtggacgg cacatccggg ccgacttcct cgatcttcgt tccactcacc cacatctgac
     1081 cggaaccttg gacgaaga
//
LOCUS       DASPLT010001259         1701 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_116451, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001259 DASPLT010000000
VERSION     DASPLT010001259.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1701)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1701)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1701
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_116451"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..525
                     /locus_tag="VEA63_12860"
     CDS             <1..525
                     /locus_tag="VEA63_12860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD84945.1"
                     /translation="PRMPGFLDQQLAVFKVVFPQDEALSLSRLAEYFVEMDRPEPAAL
                     AASVLAQSFPEDPNASIARALVFAHLERSADFEREVTQIASDVSSGRTVADWDRRVQR
                     AIVLALARRHDLARGEIATCVAEASEESLLELTSLEAHRLTTLARGYGISFADPKLAQ
                     LAAQLGAEYSGDGK"
     gene            616..>1701
                     /locus_tag="VEA63_12865"
     CDS             616..>1701
                     /locus_tag="VEA63_12865"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HYD84946.1"
                     /translation="MLLGIAVIALAVSTFLHLRTREQRSLAAPGVAARPDLRGWPDAL
                     GAEIADAENHVFERGAPVAGLARLATLYHANGFYAEAQQAYRVLRDLQPREPEWTHRA
                     AHLHATLGELPEAIALWRQTLERAPDYVPASIGCGEALLKSGKTAEAATMFSAVLARD
                     SANVYAQLGLARIDVANENWTAAQPRLETIANQTDGLLGADLLATVYERLGASERSAA
                     LRAPKKSHGLYVTIEDPWIDEIMDACYDTYRLALESGTAAIRDDRARAERLILRALEL
                     EPNDANLNFHAGQQARQRSALGSARSYLERAVRLDPALSDAWAALVSLQQQQGQMQAA
                     AQTLNQALEANPDSAVLLLERARVYKTQ"
BASE COUNT          310 a          616 c          499 g          276 t
ORIGIN      
        1 ccgcgaatgc ctggcttcct cgaccagcaa ctcgccgttt tcaaagtggt ctttccccag
       61 gacgaagcgc tgtcgttaag ccggctcgcc gaatatttcg tcgaaatgga ccggccggaa
      121 cccgccgctt tggccgccag cgtcctggct caatcgtttc cggaagatcc caacgcgtcg
      181 atcgcgcgcg cgttggtttt cgcacacctc gagcgttcgg ccgacttcga acgggaggtc
      241 acgcagatcg cgtcagatgt atcgtccgga cgcacggtcg ccgactggga tcgccgcgtg
      301 caacgagcga tcgtgctggc cctcgcccgg cgccacgacc tcgcgcgcgg agagatcgcg
      361 acgtgtgtgg ccgaggcctc ggaagagtct ctgctcgaac tcacctcact cgaagcgcat
      421 cgcctcacga cgctcgcacg cggctacggc atttccttcg cagacccgaa gctcgcccaa
      481 ctcgccgctc aactcggcgc cgaatacagc ggggacggaa aatagccgcg ccccatgcca
      541 cgcgctaaaa aaaccgccgc cgctgctgcc gcctcaagcc cgccccgcgc gatcaaccta
      601 caccggcttt ggccgttgtt actcggaatc gcagtcatcg cactcgcggt gtcgacgttc
      661 cttcatctgc gcacgcgcga gcagcgttcc ctcgccgcgc ccggtgtggc ggcccggccg
      721 gatttgcgtg gctggcccga cgcactcggg gctgaaatcg ccgacgcgga aaaccatgtc
      781 ttcgaacgcg gcgccccggt cgccggcctc gcccggctcg ccaccttgta tcacgcgaat
      841 ggattctacg ccgaggcgca gcaagcctat cgcgtcctgc gcgatctgca gccccgcgaa
      901 cccgaatgga cgcatcgcgc ggcgcatctt cacgcgacgc ttggcgagtt gcccgaggcg
      961 attgcgctct ggcggcagac gctcgaacgc gcgccagact acgtccccgc ttcgatcggc
     1021 tgcggcgagg ccctgctcaa atccggaaaa acggccgaag ccgccactat gttttccgct
     1081 gtgctcgccc gcgactctgc caatgtttac gcgcaactcg gactcgctcg catcgatgtc
     1141 gcaaacgaaa actggaccgc tgcgcagcct cggctggaga cgatcgcgaa ccaaaccgac
     1201 ggcctgctcg gcgctgatct cctcgcgacg gtctacgaac gcttgggcgc gtccgaacgc
     1261 agcgcagcgc tgcgcgcgcc gaagaaaagc cacggtctct atgtcacgat cgaagacccg
     1321 tggatcgatg agatcatgga cgcgtgctac gacacctatc gactcgcgct cgagagcggc
     1381 acagccgcca tccgcgacga tagggcgcgc gccgaacgcc tcatcctgcg cgcgctcgag
     1441 ctcgagccca atgacgccaa tctgaacttc cacgccggtc aacaggcgcg acaacgcagt
     1501 gcgctcggaa gcgcgcgctc ctacctcgaa cgcgctgtcc ggctcgatcc cgccttaagc
     1561 gacgcctggg ccgcgctcgt gtcgctgcaa caacaacaag ggcagatgca agccgccgcg
     1621 cagaccctca accaagcgct cgaggccaat ccggattccg cagtgctgtt gctggaacga
     1681 gcccgtgttt acaagaccca g
//
LOCUS       DASPLT010001260         2710 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_75394, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001260 DASPLT010000000
VERSION     DASPLT010001260.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 2710)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2710)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2710
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_75394"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            69..1157
                     /locus_tag="VEA63_12870"
     CDS             69..1157
                     /locus_tag="VEA63_12870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006983667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HYD84947.1"
                     /translation="MRGHKVKQVEIGGLFNVEDRIDPKHPIREVKRQCDAALRAMSEH
                     FDEIYSTQGRPSIPPERLLKAKVLMALYSVRSDRMFCERLNYDLLFQWFLDMNPSEAA
                     FDASTFSQNQKRLIQHEVADLFFHEVVELARAQKWISNEHFTVDGTLIEAWASMKSFK
                     PRSDQSGPGAGNPWSDFSGKPRKNDTHASTTDPEAKLVRKNDGDKARLCFGAHAVMEN
                     RSGLCVLLQVRNAIGEPESAVAVDGMIELRNRGFNPTSVGADRGYHTETFIEELREEK
                     IEPHPALMDSRQARGVKRNRRWHASQKARKRIEEIFGWMKTTGNFRKTRYRGVERTHA
                     AAQYVAAASNLIRMAKLMITAPPRMAGA"
     gene            complement(1472..>2710)
                     /locus_tag="VEA63_12875"
     CDS             complement(1472..>2710)
                     /locus_tag="VEA63_12875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC5 family protein"
                     /protein_id="HYD84948.1"
                     /translation="GLILLAKVFVPLYYRYNCTTVTELLERRYNNKHVRATVGSIFLL
                     GDVFIFLPVMLYTSSLLLRTMFGLDLPLVAIASCTAVVGAAYAIGGGLRAVAISDTYS
                     GVLVLGMALLVAFLSMQAVNWDLSNVPPERLTLIGGPDSVLPWETLLTGMIFTQLYYW
                     STNQTITQRVLAAPSIREAQKGAYAAAVVRLLIVPPMVVLPGLAAYQLFGPLGDATYG
                     RIVAAVLPSWLLGAFGAAMFAAVMTSYNATLNSAASLYVCDLHQKYINPTGSIRRLSV
                     WLQVGFALLSVAMVPLYMSAESIIQLIQQLLGLFSMPILAAFITGLLFRNVDARAVIA
                     TILFGSVFYALLSFGWPELHARNPSLPKPWHFLHLMALTVWACVGFALAVNRVVFGRR
                     AQFELSTREAWRNVFSVLRA"
BASE COUNT          603 a          822 c          862 g          423 t
ORIGIN      
        1 ctttaacagg gtgctgaaaa actctgccgc gagtttgaac gtgaaaagtg aatacgcgtc
       61 gaagtgacat gcgcggacac aaagtgaagc aagttgagat cggcgggctg ttcaacgtgg
      121 aggatcggat cgatccgaaa catccgattc gtgaggtgaa gcggcagtgc gacgcggcat
      181 tgcgagcgat gagcgagcac ttcgacgaga tttactcgac gcagggacgg ccttcgatcc
      241 cgccagagcg gctgctgaag gcgaaggtgc tgatggcgct ctactcggtg cgctcggacc
      301 ggatgttttg cgagcggctc aactacgacc tgctcttcca gtggttcctc gatatgaatc
      361 catcggaggc ggcgttcgat gcgtccacgt tcagccaaaa tcaaaagcgg ctgatccagc
      421 acgaggtcgc cgacttgttt ttccacgagg tggtcgagct ggcccgggcg caaaagtgga
      481 tctccaacga acacttcacc gtcgatggca ccctgatcga agcgtgggct tcgatgaaga
      541 gcttcaagcc gaggagtgat caatccggcc ccggcgcggg caatccgtgg tcagacttta
      601 gcggcaagcc ccgcaagaac gacacgcatg cgagcacgac tgatccggag gcgaagctgg
      661 tgcgcaaaaa cgacggtgac aaagcgcggc tgtgcttcgg tgcgcacgcg gtcatggaga
      721 atcgcagcgg actgtgtgtg ctgctccaag tgcgcaacgc catcggagag ccggagtcgg
      781 ccgtcgccgt cgacgggatg atcgaattgc gcaatcgcgg cttcaacccg acgagcgtgg
      841 gcgccgaccg cggctatcat accgagacgt tcatcgagga actgcgcgag gagaagatcg
      901 aacctcatcc tgcactgatg gactcgcgcc aagcccgcgg cgtgaaacgc aaccggcggt
      961 ggcatgcgag ccagaaggcg cgaaagcgca tcgaggagat cttcggctgg atgaagacga
     1021 cgggcaactt ccgcaaaacc cgttaccgcg gcgtcgagcg cacgcacgcc gcggcacaat
     1081 acgtcgctgc cgcctccaat ctgatccgca tggcgaaact gatgatcacc gctccgccca
     1141 gaatggccgg ggcatgatgc cccgcgcggc cgtggtgtgc ccgcgcacca cgaacacgcc
     1201 gcttaaacct gcgggacgcc tctaaaacgg cgtcgtaaac taacgcagca gctccaaatc
     1261 taccggccga tgaaccctct tcctcactct tcgcggaaat cgggaggttt ttcatcagcc
     1321 tgttaacgaa cgccgcagcg cacgcctcga ctccagggcc ttcatacggc atgcgcatcg
     1381 tcctcggcgg cctcgccggc cgcggcggat cgagcgacga agatcgccga tgtgcgcgcc
     1441 gcaaagcgtc gctgagcgat catcctcacg ctcacgcgcg caaaacggag aacacgttgc
     1501 gccacgcctc acgcgtgctc agttcgaact gcgcgcgccg gccaaacacg acacggttca
     1561 cggcgagcgc gaatcctaca cacgcccaca ccgtgagcgc catcaagtga aggaaatgcc
     1621 acggcttcgg tagcgaagga tttcgcgcgt gaagctccgg ccagccaaat gacaacaggg
     1681 cgtagaacac ggagccgaag aggatcgtcg cgatcaccgc gcgcgcgtcg acgttgcgga
     1741 acagcagtcc ggtgatgaat gcggcgagga tcggcatgct gaaaaggccg agcagttgtt
     1801 ggatgagctg gatgatgctt tccgcactca tgtagagcgg aaccatcgcc accgaaagca
     1861 gcgcgaaacc gacctgcagc cagacgctca gccggcggat gctgccggtc ggattgatgt
     1921 atttctggtg gaggtcgcag acgtaaagcg acgcggcgga attcagcgtg gcgttgtagc
     1981 tcgtcatcac cgcggcaaac atcgccgcgc cgaatgcacc gagcagccac gacggcagca
     2041 cggcggcgac gatccgcccg taagtcgcgt cgccgagcgg accgaagagt tggtaagcgg
     2101 cgaggcccgg cagcacgacc atcggcggca cgatcagcag tcgcacgaca gcggcggcat
     2161 acgcgccctt ttgcgcctcg cggatggaag gcgcggcgag cacgcgctgc gtgatcgttt
     2221 gattcgtgct ccagtaatag agctgcgtga aaatcatgcc ggtcagcagt gtctcccaag
     2281 gcagcaccga gtccggcccg ccaatcaacg tgaggcgttc aggaggaacg ttggagagat
     2341 cccagttcac cgcctgcatc gaaaggaacg ccacgagcag cgccatgccc agcacgagca
     2401 cgccgctgta agtgtccgag atcgccaccg cgcgcagccc gccgccgatc gcgtaggccg
     2461 cgccgacgac cgccgtgcaa ctcgcgatcg cgacgagcgg cagatcgagg ccgaacatcg
     2521 ttcggagcaa cagtgagctc gtgtagagca tcaccggcag aaagatgaag acgtcgccca
     2581 acaggaaaat ggagccgact gtcgcgcgaa cgtgtttgtt gttatagcgg cgctcgagca
     2641 gctcggtgac cgtcgtgcag ttgtagcgat aatacagcgg gacgaacacc ttcgcgagga
     2701 ggatgaggcc
//
LOCUS       DASPLT010001261         1871 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_138815, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001261 DASPLT010000000
VERSION     DASPLT010001261.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1871)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1871)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1871
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_138815"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            210..1409
                     /locus_tag="VEA63_12880"
     CDS             210..1409
                     /locus_tag="VEA63_12880"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HYD84949.1"
                     /translation="MKILVVDDDIVARKVLGAFLKQSNYEMQIVEDGHSAFRALTTPG
                     APLIAIVDWVMPDLTGPELCTKLRQHDFPVQPYLLMLSGRKEKSDIAIALDAGADDFI
                     SKPFNIQETQARLRVAVRAIERQLALRRQLDQLREQLRMQRALARSSRNSAQPGSTDD
                     ARPIGLGALEFHQLDTIVGSALQRIGLMETPLRQSIAPRALEIVAWTSLLLLKERQWV
                     DLFLEIEDASLSRLMAQAGGDANAPAIKREAFAATFHTALRDALLSALRTMGVDVEAP
                     LPTRALTGSDGSPRLPEEVLHRHPYQAGDGEFTLSFAAQRCPLREKTTGQLQLLDVLA
                     QPYPPASTHQVPLLRAGVVLNEQLIEKLQLFTNATAVEVPPILVHTPSPVALHYQLHS
                     TTAMLNA"
     gene            complement(1445..>1871)
                     /locus_tag="VEA63_12885"
     CDS             complement(1445..>1871)
                     /locus_tag="VEA63_12885"
                     /inference="COORDINATES: protein motif:HMM:NF015129.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD84950.1"
                     /translation="LMNLALIKGLGIEGRVYAGLFAILPEQISANSVRRRYSDFSLYP
                     AALRDLALVVDNATSADEVRKQLAKIGRTAAGNAFALERVAVFDVYQGAGLPEGKKSL
                     AFALTFRAADRTLTDDEVNVAFQKIQDEVARATSWSIRK"
BASE COUNT          353 a          610 c          569 g          339 t
ORIGIN      
        1 cgcgcgagcc tggcaacgcg atcgatatcc aaggcggtcg gttcgttcat ggcggaaaga
       61 gcggtcgagc gtgggtttcg ccggtttgaa ggcaaccggc aaaacaggcg gcgggtgcat
      121 gcgcccgcac gattcctcca atttaaacta aagatgaaac ccgatcgcgc cgatggatgg
      181 cccacccttc ccttccgttc ctgccactga tgaaaattct ggtcgttgat gacgatatcg
      241 tcgccagaaa agtgctcggc gcgttcctca agcaatcgaa ctacgagatg cagattgtgg
      301 aggacgggca cagcgctttt cgggcgttga ccacgcccgg cgcgccgctg atcgcgatcg
      361 tcgattgggt gatgcccgac ctgaccggcc cggagctctg cacgaagctg cgtcagcacg
      421 attttcccgt gcagccctat ctcttgatgc tgagcgggcg aaaggaaaag agtgacatcg
      481 ccatcgcgct cgacgccggc gcggacgatt tcatttccaa gccgttcaac attcaggaaa
      541 cgcaggcacg actccgcgtc gcggtgcgcg cgatcgagcg gcaactggcg ttgcgccgcc
      601 agctcgacca attgagggaa cagctgcgta tgcaacgcgc actcgcccgc tcatcgagaa
      661 actccgccca gcccgggagc acggacgacg cgcggcccat cggcctcggc gcgctcgaat
      721 ttcaccagtt ggacaccatc gtcggcagcg ctcttcagcg tattggtttg atggagacac
      781 cgcttcgcca gtccatcgcg ccgcgcgcgt tggaaatcgt cgcctggacg agcctgctcc
      841 tgctgaagga gcgccagtgg gttgatctgt ttctcgagat cgaagacgcg agtttgagcc
      901 ggctaatggc gcaagcgggc ggcgacgcga atgcgcccgc gatcaagcgt gaggcttttg
      961 ccgcaacgtt ccacacggcg ttgcgcgatg ccttgctctc cgcgttgcga acgatgggcg
     1021 tcgatgtgga ggcgcccttg ccgacgcgcg ccctgaccgg cagcgacgga tcgccgcgtc
     1081 tgccggaaga ggtgttgcac cgccatccct accaggcggg cgacggcgag ttcacgctgt
     1141 cgtttgccgc acaacgctgc cccttgcgcg agaaaacgac cggacaactt cagctgctcg
     1201 atgtgctggc gcagccgtat ccgccggcga gcacgcacca ggttcccctg ctccgcgccg
     1261 gcgtggtgtt gaacgaacag ctcatcgaaa agctgcaact cttcaccaac gccaccgccg
     1321 ttgaagtccc tccgatcctc gttcacacgc cctcgcccgt cgcgctgcac tatcaactgc
     1381 acagcacgac ggcgatgttg aacgcgtaac ggtcagccgc cacgacgcgg atccggccca
     1441 cccgttactt gcggatcgac cagctcgtcg cgcgcgcgac ctcgtcctga attttctgga
     1501 acgcgacgtt cacctcgtca tcggtgagcg tgcgatcggc ggcgcgaaat gtcagcgcaa
     1561 acgcgaggct cttctttcct tccggcaaac cggcgccttg gtaaacgtcg aagaccgcga
     1621 cgcgttcgag cgcgaacgca ttgcctgccg cggtgcgccc gattttcgcg agctgtttcc
     1681 ggacctcgtc ggcggacgtc gcgttgtcca ccacgagggc gagatcgcgc aacgcggccg
     1741 gataaaggct gaaatcgctg tagcggcggc gcacgctgtt cgccgaaatc tgctcgggca
     1801 gaatcgcgaa aagccccgca tagacacgcc cttcgattcc gaggcctttg atcaacgcga
     1861 gattcatcaa g
//
LOCUS       DASPLT010001262         1029 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_200012, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001262 DASPLT010000000
VERSION     DASPLT010001262.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1029)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1029)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1029
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_200012"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1029
                     /locus_tag="VEA63_12890"
     CDS             <1..>1029
                     /locus_tag="VEA63_12890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4982 domain-containing protein"
                     /protein_id="HYD84951.1"
                     /translation="NEVDYPNDPYSHPALDVARISQPTIGRYQPDRPDAMRLGAIAQR
                     LATEARKHDRSRPVTAALAGVVMSNETGYPDALDIVGYNYTEDRYRSDHESYPQRVIY
                     GSENRHSVAAWRAVEENAFIFGQFLWTGIDYLGEAGRWPSRGASAGLLDLTGAVKPRG
                     RFREALWSNRPVVYVGTERKPADDRKSTEAWPSWNYEPGTEVRVLGYTNAARARLLVN
                     GKPFGDDQPQDPETRIVHWDVPFEAGVLEMIGFDEQGAEVGRAFARTAEAPASLHVDV
                     NEAATPDAGGVSTLSVQVVDRQGVPVLSAEHEVTCKIEGAGRLLGLEAGNLRDVSDYT
                     DATHRVYH"
BASE COUNT          200 a          324 c          336 g          169 t
ORIGIN      
        1 gcaacgaagt cgactatccg aacgatccct actcgcatcc ggcgctcgat gttgcgcgca
       61 tcagtcagcc cactatcggc cgttaccagc ccgatcgtcc tgacgcgatg cgactcgggg
      121 cgatcgcaca gcggctcgcg acggaggcgc ggaagcacga tcgctcgcgt cccgtcaccg
      181 cggcgctggc cggcgtggtg atgtccaacg aaacgggata ccctgacgct ttggacatcg
      241 tcggctacaa ctacaccgag gatcgttatc ggagcgatca tgagagctac ccgcagcgcg
      301 tgatctacgg cagcgaaaac cgtcactccg tcgcggcgtg gcgggctgtg gaggaaaacg
      361 cgtttatctt cggccagttc ctttggaccg gaatcgatta cctcggcgag gcgggccgct
      421 ggccttcgcg cggcgcatcc gccggactcc tcgatctcac gggcgcggtg aaaccaagag
      481 gccgctttcg cgaggcgctg tggtcaaacc gcccggtcgt ttacgttggg acagaacgga
      541 agccggctga cgatcgcaaa tcgaccgaag cctggccgag ctggaactac gaacccggca
      601 cggaagtccg cgtgctcggt tacaccaacg cggcgcgagc gcgtttgctc gtgaacggaa
      661 agccgtttgg tgatgaccag ccgcaagatc cggagacgag gatcgttcac tgggacgtcc
      721 cgttcgaagc cggagttctg gaaatgatcg gctttgacga acaaggagcc gaagtcggcc
      781 gcgcgttcgc gcgcacggcc gaagcgccgg cgtcgttgca cgtcgacgtc aacgaagcag
      841 cgacccccga cgccggcgga gtcagcaccc tttcggttca agtcgtggat cgccaaggcg
      901 tgcccgtgtt gagcgcggag cacgaggtga cgtgcaagat tgaaggcgcc ggccgcttgc
      961 tgggcctcga agcgggcaat ctccgagacg tgtccgacta cacggatgca acgcaccgcg
     1021 tctatcacg
//
LOCUS       DASPLT010001263         1786 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_116490, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001263 DASPLT010000000
VERSION     DASPLT010001263.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1786)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1786)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1786
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_116490"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1086)
                     /locus_tag="VEA63_12895"
     CDS             complement(<1..1086)
                     /locus_tag="VEA63_12895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HYD84952.1"
                     /translation="MLQSLRIRNLALLEEVALDFEAGFTAVTGETGAGKSILLGALSL
                     LAGERADKTIIRQGAPAVETEASLFFEDSAKIDAVLAELDLPPCEDGLLILKRSLPRE
                     KAPKITVNGGLATLSALQRLGEHWVDFHGPSEPRRLLKENCQLELLDLFGRAESALSA
                     YRKSYDIWRERVAERERIANETKLSPDQIEFLQNQLAAIEELELTDEAIETLERDFAR
                     MSRAQELIELTQALEAGLTGEEGVQLRLAALLREARQVESIDAASKPLADRLASVAVE
                     INDLSAEFSTLSQELQFDPEQAETLASRMNTWLELKRKHGGDIGAVLAARDEMRRRLE
                     VQGDLEGTLARLEKQIAEAERAAKKEAQ"
     gene            1302..>1786
                     /locus_tag="VEA63_12900"
     CDS             1302..>1786
                     /locus_tag="VEA63_12900"
                     /inference="COORDINATES: protein motif:HMM:NF012544.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HYD84953.1"
                     /translation="MSNQHTVGSVTYTKADNSYFEKRGLRRHARVWSLWALGVGAVIS
                     GHFSGWNLGLGQGGWGGLCVAAVLIAIMYLGLIYSLAEMSPALPHTGGAYSFARSAMG
                     PWGGFVTGLAESIEYIMTPAVIVFFIGSYLSAIFETPAAAQPLWWIGAYVVFVALNYW
                     GV"
BASE COUNT          292 a          663 c          468 g          363 t
ORIGIN      
        1 ctgcgcttcc ttcttcgccg cccgctccgc ctccgcgatc tgcttttcca atcgcgcgag
       61 cgtcccctcg agatcgccct gcacctccag ccgtcgacgc atttcgtcgc gcgccgccag
      121 caccgcgccg atatcgccgc cgtgttttcg cttcagctca agccacgtgt tcatccgcga
      181 cgccagcgtt tccgcttgtt cgggatcgaa ctgcaattcc tggctcaacg tcgaaaactc
      241 cgccgaaaga tcgttgatct cgaccgcgac cgacgccagc cggtccgcga gcggcttgct
      301 cgccgcatcg atgctctcga cctgccgcgc ctcgcgcaac aacgccgcca gccgcaactg
      361 cacgccttcc tcccccgtca gcccagcctc gagcgcctga gtgagctcga ttaattcctg
      421 cgcccggctc atccgcgcga aatcccgctc gagcgtttcg atcgcttcgt ccgtcaactc
      481 cagctcctcg atcgccgcga gctggttctg caaaaactcg atctgatccg gcgaaagctt
      541 cgtctcgttg gcgatccgct cgcgctcggc cacgcgctcg cgccagatgt catagctttt
      601 tcgatacgcc gacagcgcac tctccgcgcg gccgaacaag tcgagcagtt caagctggca
      661 gttctccttc aacaaccgcc gcggttcgct cggtccgtga aaatccaccc agtgctcgcc
      721 gagccgctgc aacgccgaga gcgtcgcgag cccgccgttg accgtgattt tcggcgcttt
      781 ctcgcgcggc aggctgcgtt tcaaaataag caaaccgtcc tcgcagggcg gcagatcgag
      841 ctccgcgagc accgcatcga tcttcgccga atcctcgaaa aacaaactcg cctccgtctc
      901 caccgcgggc gcgccctggc gaatgatcgt cttgtccgcg cgctcccccg ccagcaggct
      961 caacgccccg agcaagatgc tcttgccggc gcctgtctca cccgtgacgg ccgtgaagcc
     1021 ggcctcgaaa tccagcgcga cttcctcgag caaagccagg ttgcgaatgc gaagcgattg
     1081 aagcatcgcc catggaggca caaaaaccgc ccgagtttca agtggcgttc tgctggcttt
     1141 tgccaacatc gtttcgccca gtcttcttca cccttgcacg caggctaccc gcttttcgcg
     1201 cgtctcatcc cctcgccagt gccaatgacc ggcatgagtc gtgctcgtcg actcgcattc
     1261 gatcagcctt cgagccgcgc actttccaac taacgactca catgagcaac caacacaccg
     1321 tgggctccgt cacctacacg aaagcggaca actcctattt cgaaaaacgc ggactgcgcc
     1381 gacacgcgcg cgtctggtcg ctttgggcgt tgggcgtcgg cgccgtcatc tccggacact
     1441 tctccggctg gaatctcggc ctcggccaag gcggttgggg cggcctctgc gtcgccgccg
     1501 ttctcatcgc gatcatgtat ctcggcctga tttacagcct cgccgagatg tcgcccgcgc
     1561 tccctcacac cggcggcgcg tattccttcg cccgcagcgc gatgggccct tggggcggct
     1621 tcgtcaccgg ccttgccgag agcatcgaat acatcatgac gcccgccgtc atcgtgttct
     1681 tcatcggctc ctacctgtct gctatctttg aaacgcccgc cgccgcacaa ccgctttggt
     1741 ggatcggcgc ctacgtcgtt ttcgtcgcgc tgaattactg gggcgt
//
LOCUS       DASPLT010001264         1068 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_302308, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001264 DASPLT010000000
VERSION     DASPLT010001264.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1068)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1068)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1068
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_302308"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            34..1008
                     /locus_tag="VEA63_12905"
     CDS             34..1008
                     /locus_tag="VEA63_12905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD84954.1"
                     /translation="MKVLYALCAVLLLFGCNRTETALSVPPSFYPSAIAYDAARGLHY
                     IGSFANGEIAVVDEAGDRVGTLRAATPGDRVVRLAIEVRERKLWAATSNAIEVFHLDR
                     PDLLRQIYPVGTSMQYADFAVAPHGVVYLLDRSEGLVRRLDTRRAELHTVARLPREAM
                     TGGGVHCSLTGEPGVAHVAGGALALMPGEELLLAAYDERLYRVAIATGEVAQIPLHGP
                     LTYVTQLIHRGTAFGRHEIVAIRGEASRITHLALDADLQRLYRQESFHAASSAPIAAA
                     FDGTHVRMIAGPLRHHPVYCGDGRPTAPLRIIAHAPKPVVSHQVLAMD"
BASE COUNT          173 a          354 c          346 g          195 t
ORIGIN      
        1 aagtaccgtg gcggcctaga tgcgaggtcc gccatgaaag ttctatacgc gctgtgtgct
       61 gtgcttctcc tgttcggctg caacagaacc gaaacggcgc tgtccgtgcc gccgtcgttc
      121 tacccgtccg ccatcgctta cgacgcagcg cgcgggcttc actacatcgg cagcttcgcc
      181 aatggggaga tcgcggtcgt cgatgaagcg ggcgatcgcg tcgggacgtt gcgcgcggcc
      241 acgccgggcg atcgcgtcgt gcggctcgcg atcgaagtgc gggagcgcaa gttgtgggct
      301 gcgacgagca acgccatcga agtgttccat ctcgaccgtc cggatttgct gaggcagatc
      361 tacccggtcg gtacctcgat gcaatacgcg gatttcgccg tcgcgccgca cggagtggtc
      421 tatctgctcg accgatctga aggcctcgtg cgccgactcg atacgcggcg agcggaactg
      481 cacaccgtcg cacgtcttcc gcgcgaggcc atgaccgggg gcggagtcca ttgcagcctg
      541 acgggcgaac cgggggtggc ccatgtcgca ggcggcgcgc tcgcgctgat gcctggcgaa
      601 gagcttcttc tcgctgcata cgatgaacgg ctctatcggg tcgccatcgc gaccggcgag
      661 gtcgcccaga tcccgctgca cggaccgctc acatatgtga cgcaattgat ccatcgcgga
      721 acggcattcg gccgccacga aatcgtggcg atccgcggcg aagcgagccg catcacgcat
      781 ctcgcgctcg atgcggactt gcagcggctg tatcggcagg agtcgttcca tgccgcctcg
      841 tctgcgccga tcgcggctgc gttcgatggc acgcacgtgc gcatgatcgc cggaccgctg
      901 cggcatcatc cggtctactg tggcgacgga cggcccactg cgccgctccg gatcatcgcg
      961 cacgccccga agccggttgt atcgcaccaa gtgctcgcga tggactgaac cgctgttgac
     1021 gcgcggcaac aggccgccta ccttctgctg catgtctcgc gtctacca
//
LOCUS       DASPLT010001265         4042 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_179715, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001265 DASPLT010000000
VERSION     DASPLT010001265.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 4042)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4042)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4042
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_179715"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..346
                     /locus_tag="VEA63_12910"
     CDS             <1..346
                     /locus_tag="VEA63_12910"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HYD84955.1"
                     /translation="AAKRMAGWAPTRIFEAARRTLATLLSCDERALADAIVDWRTHDW
                     SADPWTRGAYSFSVAGRENAPAKLARPVGGTLFFAGEATADPLELGTVGGALTSGERA
                     AKEILAKLKRRG"
     gene            390..839
                     /locus_tag="VEA63_12915"
     CDS             390..839
                     /locus_tag="VEA63_12915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HYD84956.1"
                     /translation="MATLYETLRADIIGAMKARDSATATTLRTADAAIQRSAMDSSKP
                     IDDALVTTTLRKAVKNLTDAKAEFAKGGRDDLVAANDVEIRILEKYLPQALDAAKLEA
                     LIVAAIQETGATSKKEMGKVGGALKQKPEAGLIDFGAASKLVQAKLP"
     gene            complement(925..1755)
                     /locus_tag="VEA63_12920"
     CDS             complement(925..1755)
                     /locus_tag="VEA63_12920"
                     /inference="COORDINATES: protein motif:HMM:NF036491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD84957.1"
                     /translation="MKHKVVTDLELMLKNKSKLGRLERFHDAIQKKEEAFSLFNLGYL
                     DLENRAAAESLYWQICEQIAKEGRDSGYQPEELHDLNTLLADQYVCNFSVFQSLLDHW
                     ALKQLFPIAPLHRLNEKPSVNAILVDITCDSDGKISSFIDLQDVKDYITLHPLNKEPY
                     YLGIFLTGAYQDIMGDLHNLFGRVNEVHVFLEDDEPNGFYIEEALSGSRIADVIEGVQ
                     YQQEDLCRKMKAQIDAATRKDMVKPREGVRLLELYESQMLNKTYLNIEPKNVRKKKHK
                     "
     gene            complement(1756..2871)
                     /gene="speA"
                     /locus_tag="VEA63_12925"
     CDS             complement(1756..2871)
                     /gene="speA"
                     /locus_tag="VEA63_12925"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF003763.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008792 - arginine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HYD84958.1"
                     /translation="MKNKSASAWSAAKSEEHYGFKRWGSGHISVDDEGFVNMQPRADA
                     RGIRVLDVVHEALGMGLKAPMVIRFQDLLRHRVIQINEMFAKAIKEEGYKGEYRGVFP
                     IKVNQLREVVDEIVAAGKDFNYGLEAGSKPELMIALAMHEGTQRLIICNGYKDHDYIR
                     LALLGRKLGKKIIIVVEQLQELDDIIRLSAETGVKPMIGFRAKLQTRGEGKWAMSTGD
                     NAKFGLNTAEILFACEKLKAAKLSSALRLVHFHIGSQVPNIITIKNAVIEATRFYCQL
                     SRMGFPMGYLDVGGGLGIDYDGSRTNFESSMNYSMEEYARDVVFNIREICTASDVDVP
                     DIVSESGRAIVAPHSLLVVEVFERINKRESLGQQHQP"
     gene            2961..3311
                     /gene="yajC"
                     /locus_tag="VEA63_12930"
     CDS             2961..3311
                     /gene="yajC"
                     /locus_tag="VEA63_12930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HYD84959.1"
                     /translation="MTKMSLTLDAPLFLAQQPAPGPGAGGMSQLIFFGLLFAAMYFLM
                     IAPQRKKQKAHEKMLKELQSGDEIVTSGGIYGTITNVKEDRFVVRIADTTKIEVGKAF
                     VSGVVSKADGEAKK"
     gene            3403..>4042
                     /locus_tag="VEA63_12935"
     CDS             3403..>4042
                     /locus_tag="VEA63_12935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecDF"
                     /protein_id="HYD84960.1"
                     /translation="MFRRNLWKIAICLAIVVWAVGTLLPLQDQPFTDYVKAEATAKPA
                     EFNALLTEAVAMKTNGQALSEFVALKQIARERRIDLSQYFPEVRLEETLRNVEKRNDI
                     LLNELLRRSKSNLQLGLDLKGGVAFTLEVDEKAASDEPTSAREEKLQKAIEIISERIN
                     AFGVAEPIVRPVGDNRIEVQLPGVNTRDNPEVVDAVKKPARLDFRIVHPTQSP"
BASE COUNT          779 a         1084 c         1370 g          809 t
ORIGIN      
        1 ggcggcgaaa cgcatggcgg gttgggcgcc gacgcggatt tttgaggcgg cgcggcgaac
       61 gctggcgacg ctgctctcgt gtgatgagcg cgcgctggcg gacgcgattg tcgactggcg
      121 cacgcacgat tggtcggcgg atccgtggac gcgcggggct tatagtttct cggtcgcggg
      181 tcgggagaac gcgccggcga agctggcgcg tccggtcggc ggcacgttgt ttttcgcggg
      241 tgaagcgacg gcggatccgt tggagctcgg cacggtcggc ggggcgctca cgagcggcga
      301 gcgtgcggcg aaggagattc tcgcgaagct gaagcggcga ggttgagcgc ggcgctggac
      361 acggcccgtt tggcgcgcat ggtcgtggca tggcgacgct ttacgagacc ttgcgagcag
      421 acatcatcgg cgcgatgaaa gcgcgcgaca gcgcgacggc gacgacgttg cggacggcgg
      481 acgcggcgat tcagcggagt gcgatggatt cttcgaagcc catcgacgac gcgctggtca
      541 cgacgacgct gcgcaaggcc gtcaagaatc tcaccgatgc caaggcggag ttcgcgaagg
      601 gcggacgcga cgatctggtg gcggcgaatg acgtcgagat ccggattctg gaaaaatatc
      661 tgccgcaggc gctcgacgcg gcgaagttgg aggcgttgat cgtggcggcg attcaggaaa
      721 cgggcgcgac gtcgaagaag gaaatgggaa aagtgggcgg cgcgctgaag caaaagccgg
      781 aggccgggct gatcgatttc ggtgccgcga gcaagctggt gcaggcgaag cttccgtgac
      841 gagcgcgccg gccgaagccg gtgacgagcc cgaacagggc ggctgcgaga gccgccccta
      901 cttttttgag cggcgcgcgc gtggctactt gtgctttttc ttccggacgt tcttcggctc
      961 gatgttcaag taagttttgt tgagcatctg gctctcgtag agctcgagga ggcggacacc
     1021 ttcgcgcggc ttgaccatgt ctttgcgcgt ggcggcgtcg atctgcgctt tcatctttcg
     1081 gcagagatct tcctgctggt attgcacgcc ttcgatgaca tcggcgatgc ggctgccgct
     1141 gagcgcttcc tcgatgtaga aaccgttggg ctcgtcgtct tcgaggaaaa cgtgaacctc
     1201 gttcacgcgg ccgaaaagat tgtggaggtc gcccatgatg tcctggtagg cgccggtgag
     1261 aaagatgccg aggtagtaag gctccttgtt cagcggatgc agcgtgatgt aatccttcac
     1321 gtcctgcaga tcgatgaacg aggaaatctt cccgtcggag tcgcaagtga tgtcgacaag
     1381 gatggcgttg accgacggtt tttcgttgag gcgatgcagc ggcgcgatgg ggaagagctg
     1441 cttgagcgcc cagtgatcaa ggagcgattg gaagaccgag aaattacaaa cgtattggtc
     1501 ggcgagcagc gtgttgaggt cgtggagctc ttcgggctgg tagccggagt cgcgaccttc
     1561 tttggcgatc tgttcgcaga tttgccagta aagcgattcg gcggcggcgc ggttttcgag
     1621 gtcgaggtaa ccgagattga agagcgagaa cgcctcctct tttttctgga tcgcgtcgtg
     1681 gaaacgttcg agccggccga gcttcgattt gtttttcaac atcagctcga gatcggtgac
     1741 gaccttgtgc ttcacctacg gctggtgttg ctggccgagg gattcgcgtt tgttgatacg
     1801 ttcgaagacc tcgacaacca ggagcgagtg gggcgcgacg atggcgcgac cggattcgct
     1861 gacgatgtcg gggacgtcga cgtcggaggc ggtgcagatc tcgcggatgt tgaagacgac
     1921 gtcgcgcgcg tattcctcca tcgagtagtt catcgagctc tcgaagttcg tgcgcgagcc
     1981 gtcgtagtcg atgccgagtc cgccgccgac atcgaggtaa cccatcggga aacccatgcg
     2041 cgagagctgg cagtagaaac gcgtcgcttc gatgacggcg ttcttgatcg tgatgatgtt
     2101 cgggacctgt gagccgatgt gaaagtgaac gaggcggagc gcggaggaga gcttcgcggc
     2161 tttcagcttt tcgcaggcga acaggatttc ggcggtattg aggccgaact tcgcgttgtc
     2221 gccggtggac atggcccact tgccttcgcc gcgcgtctgg agtttcgcgc gaaaaccgat
     2281 catcggcttc acgccggttt cggcggagag gcggatgatg tcgtcgagtt cctggagttg
     2341 ctcgacgacg atgatgatct tcttgccgag cttgcggccg agaagcgcga gacggatgta
     2401 atcgtgatcc ttgtagccgt tgcagatgat gagccgctgc gtgccttcgt gcatcgcgag
     2461 cgcgatcatg agctcgggtt tggagccggc ttcgaggccg tagttgaagt ccttgccggc
     2521 ggcgacgatc tcgtcgacga cttcgcggag ctggttgacc ttgatgggaa acacgccgcg
     2581 gtattcgcct ttgtagccct cttccttgat cgctttcgcg aacatctcgt tgatctgaat
     2641 gacgcggtgg cgaaggaggt cttgaaaacg gatcaccatc ggggccttga ggcccatgcc
     2701 gagcgcttcg tgaacgacgt cgaggacgcg gatgccccgg gcgtcggcgc gcggctgcat
     2761 gttcacgaat ccttcgtcgt cgaccgagat gtggcccgag ccccagcgct tgaaaccata
     2821 atgctcttca gacttggcgg ccgaccaggc ggatgcggat ttgtttttca acgaggggaa
     2881 ggcggggagt tatggcttgc gttcgcggaa gcggaatcag ttagatgcgt gttttctttt
     2941 ttcaaatcaa cgaacacgca atgacgaaaa tgtccctgac cctcgacgcg cctcttttcc
     3001 tcgcccagca acccgcgccg ggacccggcg ccggcggcat gagtcagctg atctttttcg
     3061 gactgctctt cgccgcgatg tatttcctga tgatcgcgcc gcagcgtaag aaacagaagg
     3121 cgcacgagaa aatgttgaag gagctgcagt cgggcgacga gatcgtgacg agcggcggca
     3181 tttacggcac gatcaccaac gtgaaggaag atcgtttcgt ggtgcgcatc gcggacacga
     3241 ccaagatcga ggtgggcaag gcgttcgtga gcggcgtcgt cagcaaggct gacggcgagg
     3301 cgaagaagta atttcgagtt tcggccatgt ttttagccgt cgggctggcg ccgtgaacgg
     3361 cggggctgcg acggcttcat cgattttaaa cccaatatag ccatgttcag acgcaacctc
     3421 tggaagatcg ccatctgcct tgccatcgtg gtgtgggccg ttggaacact tctacccctg
     3481 caagaccagc cgttcaccga ttacgtgaag gctgaagcga ccgccaaacc ggccgagttc
     3541 aacgccctcc tcacggaagc ggtcgcgatg aaaaccaacg gtcaggcgct cagtgagttc
     3601 gtcgcgctca agcaaatcgc gcgcgagcgc cgcatcgatc tctcgcaata tttccctgag
     3661 gtccgtctcg aggagacgct gcgaaatgtc gaaaagcgaa acgacattct actgaacgag
     3721 ctgctgcgtc gctccaagag caacctgcaa ctcggcctcg acctcaaagg cggcgtggcc
     3781 ttcacgctgg aagtcgacga gaaggcggcg agcgacgagc cgacgagcgc gcgcgaggag
     3841 aaattgcaga aggcgatcga gatcatcagc gaacgcatca acgctttcgg cgtcgcggaa
     3901 ccgatcgtgc gcccagtggg cgacaatcgc atcgaggtgc aattacccgg cgtgaacacg
     3961 cgcgacaacc ccgaggtggt cgatgcggtg aagaagccgg cacgactgga tttccgcatc
     4021 gttcatccga cgcaatcgcc gc
//
LOCUS       DASPLT010001266         1078 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_96101, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001266 DASPLT010000000
VERSION     DASPLT010001266.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1078)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1078)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1078
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_96101"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..313
                     /locus_tag="VEA63_12940"
     CDS             <1..313
                     /locus_tag="VEA63_12940"
                     /inference="COORDINATES: protein motif:HMM:NF013630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HYD84961.1"
                     /translation="ASARVSISSLASKFKTTPEVLREANNIPARTRLKAGSTILVPKL
                     ASSTAGDIGESIVENAVMALEPERAGKRKSSRATSKFRAKSGKKSSKRSRSATNNRKK
                     R"
     gene            613..>1078
                     /locus_tag="VEA63_12945"
     CDS             613..>1078
                     /locus_tag="VEA63_12945"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HYD84962.1"
                     /translation="MAFLQGKKILITGLLSNRSIAYGIAKACHREGAELAFTYVGDRF
                     KDRITEFAAEFDSKLVFDCDVASDEQIEKLFADLRTTWEHLDGFVHAIGFAPREAIAG
                     EFLDGLTRENFRIAHDISAYSFPAMAKAALPMLREGSSLLTLSYLGAVRAIPY"
BASE COUNT          208 a          362 c          324 g          184 t
ORIGIN      
        1 cgccagcgcg cgggtgtcga tctcctcgct cgcatcgaag ttcaagacca cgccggaagt
       61 gctgcgcgaa gcgaacaaca tccctgcccg cacgcgcctg aaggccggct ccaccatcct
      121 ggtgccgaag ctggcttcgt ccaccgccgg cgacatcggc gagagcatcg tcgagaacgc
      181 cgtgatggcg ctggagccgg aacgcgcggg caagcgcaag tcgtcgcgcg ccacatcgaa
      241 gttccgcgcc aagtctggca agaagagcag caagcgttcg cgcagcgcga ccaacaaccg
      301 caagaagcgt taagcatgtc acccccgcgc aggcgggggc ccatactgag ttaccgaagt
      361 tgctcgctca gtaggggtcg ccgcgttcgc ggggatgacg cgctgccgaa gttgtttgct
      421 cagtatgggt ccccgcgttc gcggggatga cgcgcagccg aagttgttcg ctcagtatgg
      481 gtccccgcgt tcgcggcgat gacgcgcttc caaagttgca gttacagcaa actcccccta
      541 caatctcgaa ttggcccggc tcgaccggcg ctcacccgtc acgaaaccaa agacgtatca
      601 tcggagtcca acatggcgtt cttgcaaggc aaaaaaatcc tcatcaccgg cttgctgtcg
      661 aaccgttcga tcgcgtacgg catcgccaag gcctgccacc gcgaaggcgc ggagctggcg
      721 ttcacctacg tcggtgaccg cttcaaggac cgcatcaccg aattcgctgc cgagttcgac
      781 agcaaactgg tcttcgactg cgacgtcgcg agcgacgaac agatcgagaa gctgtttgcc
      841 gacctgcgca ctacgtggga acacctcgac ggtttcgtgc acgcgatcgg cttcgcgccg
      901 cgcgaagcga tcgccggcga attcctggat ggcctgaccc gcgagaactt ccgcatcgcg
      961 cacgacattt ccgcgtacag cttccctgcg atggccaagg ccgcgctgcc gatgctgcgc
     1021 gaaggctcgt cgctgctgac cctgtcctac ctgggcgccg tgcgcgcgat cccttact
//
LOCUS       DASPLT010001267         2544 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_260681, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001267 DASPLT010000000
VERSION     DASPLT010001267.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 2544)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2544)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2544
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_260681"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..349)
                     /locus_tag="VEA63_12950"
     CDS             complement(<1..349)
                     /locus_tag="VEA63_12950"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007363397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HYD84963.1"
                     /translation="MKLTIVSETFPPEINGVAMTFGHLTRELAHRGHEITVWRPRRDD
                     LANASSSVRFAEVLVRGIPIPGYPLLRLGLPARSALIRRWREQRPDLVHVVTEGPLGA
                     SAITAARALGIPVT"
     gene            562..>2544
                     /locus_tag="VEA63_12955"
     CDS             562..>2544
                     /locus_tag="VEA63_12955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140658 - ATP-dependent chromatin
                     remodeler activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2-related protein"
                     /protein_id="HYD84964.1"
                     /translation="MEFWFDRLEHDWANAFTAEQLEQGRRIYVDGHVRELELTAKDAI
                     IHRRVEKKDEYAVIEWGENGISVRSSTTDIDVAHALAVAGLHEIEELVADEISPLPGD
                     SPAGESANGHTNGHTNGHGAPAGVSGPKPGSIAAGGGRSTAQAGGAATGPSRTLLLVF
                     KTKTAGLTFQAYWAEADGKTRHPALGAAAHADGNGHVSSGERAKLIGLAAYARKAHFH
                     YHQETGVYLMESLVEIPNFLKTVLPAWRRMFAIELDDKAGNLVKGTRAIELEAVAEPM
                     AAGADASGAGGSASDVAGLNLRWIFRAGERMLTDSEVNTLLKKGGAPVILPSLGIVSL
                     STERWASFSAWRDNLDEAQATGALSPYLVFSLFNDARLKLTLSPEIEAWRQRVLATPE
                     APPLLPEILRPYQRRGVEWLAHLCDVGCHGLLADEMGLGKTLQVITLLATRPVGAKPS
                     LIVCPASVVPVWREEIAKFQPGLVVDVLKTGHDFTTRSDAVIWLASYTQLRKHREILP
                     AHEFGYAVLDEGQFIKNPDAKVTQTCFAIRAKHRIVLTGTPLENRQLDLWSIFRFLLP
                     GLLGSRTSFENALNADRVATLERLRAQLAPFILRRTKKEVATELPPKVEMELLCPLTD
                     VQRAEYARICSEGLERLGDDVGAAMREKSFGFLALLT"
BASE COUNT          464 a          691 c          946 g          443 t
ORIGIN      
        1 acgtgaccgg aatcccgagc gcgcgcgcgg cggtgatggc ggaggcgccg agcggtcctt
       61 cggtgacaac atggacgaga tcggggcgtt gttcgcgcca gcgacggatc aacgcgctcc
      121 gagcgggaag accaagacgg agcaggggat aaccgggaat cggaatgccg cgcacgagca
      181 cctcggcgaa cctgactgac gacgaggcat tcgcgagatc gtcgcgtctg ggacgccaca
      241 cggtgatctc gtggccgcgg tgcgcgagct cccgggtaag atggccgaac gtcatggcca
      301 ccccgttgat ctcgggtggg aaggtttcgg acacgatggt gagtttcacg acgtcggcgg
      361 aaggagagag cgttggattg cgcgggcagt gtgggggcgc gacgtgccga tcgggtggcg
      421 gtcccgttac gaaccggtta aaaaaatatc ggagggaaaa ttcgttactt cgggattgca
      481 ccgccttggc cgaattctac cgtcgccgct tttacgtgaa tgcagtcgca cggtcctaag
      541 cgcgtataca caccgcagtc cttggaattt tggttcgacc ggctcgaaca cgactgggcc
      601 aatgcattca ctgccgagca gttagagcag gggcgccgca tctatgtgga cggccacgtg
      661 cgcgagctcg agttgacggc gaaggacgcg atcatccacc gacgggtgga gaagaaggat
      721 gaatacgcgg tgatcgagtg gggggagaat gggatttccg tgcggtcttc gaccacggac
      781 atcgacgtgg cgcatgcttt ggcggtggcg ggtttgcacg agattgagga gctggtggcg
      841 gatgaaattt caccgctgcc gggcgattcg ccggcgggcg aaagcgcgaa cggtcacacc
      901 aacggtcata cgaacggtca cggtgcgccg gcgggcgtaa gcgggccgaa gccggggtcg
      961 attgcggcgg gcggcggtcg cagcacggca caagctggcg gcgcagcgac cgggccatcg
     1021 cgcacgcttt tgctcgtgtt caaaacgaag acggcgggcc tgacgtttca ggcgtattgg
     1081 gcggaggcgg acggcaagac gcgtcacccg gcactcggtg cggcggcgca cgccgatggc
     1141 aacgggcacg tgtcgtccgg cgaacgcgcg aagctgatcg ggctcgcggc gtatgcgcgc
     1201 aaggcgcact tccactatca tcaggaaacg ggcgtctact tgatggagtc gctcgtcgag
     1261 attccaaatt ttctaaaaac ggtgctgccg gcctggcggc ggatgttcgc gatcgagctc
     1321 gacgacaaag ccggaaatct ggtgaagggc acgcgtgcga tcgaactcga ggcggtcgcc
     1381 gagccgatgg cggcgggcgc cgatgcgagc ggcgcgggcg gaagcgcgag cgatgtggcg
     1441 gggttgaacc tgcgctggat cttccgcgcg ggcgaacgga tgttgaccga ctccgaggtg
     1501 aacacgctgc tgaaaaaagg cggcgcgccg gtgattctgc cgagcctcgg catcgtgtcg
     1561 ctgtcgaccg agcgttgggc gtcgttcagc gcgtggcgcg acaatctcga cgaagcgcag
     1621 gcgacgggcg cgttgtcgcc gtatctggtg ttttcgttgt tcaacgacgc gcggctgaag
     1681 ctgaccttgt cgccggagat cgaggcgtgg cgtcaacgcg tgctggcaac gcctgaggcg
     1741 ccgccgctgc tgccggagat cttgcggccg tatcaacggc gcggcgtgga gtggctcgca
     1801 cacctgtgcg acgtgggctg ccacgggttg ttggccgacg agatgggttt gggcaaaacg
     1861 ctgcaagtga tcacgctgct ggcgacacgt cccgtggggg cgaagccgag cctgattgtg
     1921 tgtcccgcga gcgtggtgcc ggtgtggcgc gaggagatcg cgaagtttca gccgggattg
     1981 gtggtcgatg tattgaagac cgggcacgat ttcacgacgc gttcggatgc ggtgatctgg
     2041 ctggcgagct acacgcagtt gcggaagcac cgcgagatac tgccggcgca cgagttcggc
     2101 tatgcggtgc tggacgaagg tcagttcatc aagaatccgg acgcgaaggt gacgcagacg
     2161 tgtttcgcga ttcgggcgaa gcatcggatc gtgcttacgg gcacgccgct ggagaaccgg
     2221 cagctcgatc tgtggagcat tttcaggttt ttgcttccgg ggctgctggg ctcgcgcacg
     2281 agtttcgaga acgcgctcaa cgcggatcga gtggccacgc tggagcggct gcgcgcgcag
     2341 ctcgcgccgt ttattttgcg ccgcacgaaa aaagaagtgg ccacggagct gccgccgaag
     2401 gtggaaatgg agctgctgtg cccgctcacc gacgtgcagc gcgcggagta tgcgaggatt
     2461 tgttccgaag gactggagcg gctgggcgac gacgtgggcg cggcgatgcg cgagaaatcg
     2521 ttcgggttcc tcgcgctgct gacg
//
LOCUS       DASPLT010001268         1000 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_158067, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001268 DASPLT010000000
VERSION     DASPLT010001268.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1000)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1000)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1000
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_158067"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..222)
                     /locus_tag="VEA63_12960"
     CDS             complement(<1..222)
                     /locus_tag="VEA63_12960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD84965.1"
                     /translation="MKKTLTLLVLAVGLTASAPASYRIGQQEIPAVEIKSDAASFEVH
                     SGHPRLFFRDTDLPAIRERIAGDYQSEWRE"
     gene            complement(230..>1000)
                     /locus_tag="VEA63_12965"
     CDS             complement(230..>1000)
                     /locus_tag="VEA63_12965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD84966.1"
                     /translation="FVGTNLFSVGEQPGQGRNGLKGWGVLAVDDVLYLWFGHADKKGG
                     AAHLAWSRDGARTWTFADWSFPEFGLVGFINFGRGYDGARDNFVYSYSHDAPKADQPA
                     DRFILMRAPKDRLTERAAWEFFSGRDAKGEPTWTSDMNRRSAVFENPDQCLRSAITYN
                     PALRRYLWWQAIPQPRGHKDRGDTRFEGGFAVYDAPEPWGPWTTAYYTEKWDVGPGEH
                     GDFPAKWMSDDGRTVHLVFSGEDAFSVRRATLVVSDQP"
BASE COUNT          199 a          322 c          303 g          176 t
ORIGIN      
        1 ttccctccac tccgactgat aatcgcctgc gatccgttcg cgaatcgccg gaagatcggt
       61 gtcgcggaaa aacagccgcg ggtgcccgga atgaacttcg aagctcgcgg cgtccgattt
      121 gatttccacc gccggaatct cctgctgacc gatgcggtag ctcgcaggag cggaggccgt
      181 cagccccacc gcaagcacga gtagcgtgag cgttttcttc attttatagt cagggttgat
      241 ccgacacgac gagcgtcgcg cgacgaacag agaaagcgtc ttcgccagag aaaacgaggt
      301 gcacggtcct gccgtcgtcg ctcatccact tggcgggaaa atcgccgtgt tcgccggggc
      361 cgacatccca cttttccgtg taatacgccg tcgtccaagg cccccaaggc tcaggcgcgt
      421 cgtaaaccgc gaagccgcct tcgaaccgcg tgtcgccgcg atccttgtga ccgcgcggct
      481 gcggaattgc ctgccaccaa aggtagcgac gcaacgcggg gttgtaggtg attgctgagc
      541 gcaggcactg atcgggattt tcgaaaacgg cggagcgacg attcatgtcg gaggtccagg
      601 ttggctcacc cttcgcatcg cgaccggaaa agaactccca cgcggcgcgt tccgtcaggc
      661 gatccttggg cgcgcgcatc aggataaaac gatcggccgg ctggtcggcc ttcggcgcgt
      721 cgtgagaata cgaatacacg aagttgtcgc gcgcaccgtc gtagccgcga ccgaagttga
      781 tgaagccgac gagcccgaac tccgggaagc tccaatccgc gaacgtccac gtgcgggcgc
      841 cgtcgcgcga ccacgcgaga tgcgcggcgc cgcctttctt gtcggcgtgt ccaaaccaca
      901 aatacagcac gtcgtcgacc gcgaggacgc cccacccttt caaaccgttg cgcccctgcc
      961 cgggttgttc gcccacggaa aacaggttcg tgccgacgaa
//
LOCUS       DASPLT010001269         1031 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_158087, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001269 DASPLT010000000
VERSION     DASPLT010001269.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1031)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1031)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1031
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_158087"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..629
                     /locus_tag="VEA63_12970"
     CDS             <1..629
                     /locus_tag="VEA63_12970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HYD84967.1"
                     /translation="NGAGKTTTLRSIAGIIPATAGTVRIAGHDLASDPVAAKRALAFF
                     PDEPRLFEYLTVRQHLAFVAKIYGIHDHATLAQPLLDELEIADKADDLPGALSRGMKQ
                     KLAIACGLLHAPSVMFFDEPLTGLDPLGIRRMKDSILKRARAGAAIVLSSHLLHLLEE
                     VCSHVLILKQGAKIADGTLAEVAARFSNGEPDVSLEEIFIRATGGTEN"
     gene            757..>1031
                     /locus_tag="VEA63_12975"
     CDS             757..>1031
                     /locus_tag="VEA63_12975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD84968.1"
                     /translation="MLRALLYLRLTSLKNLLLTRLRRLRQPKYLAGALVGGAYFYFFF
                     FRHAHTATQKNPGAFFTLPADSAPLFLGVGALALLAILLGMWIFPSG"
BASE COUNT          191 a          376 c          262 g          202 t
ORIGIN      
        1 ccaatggcgc gggcaaaacc acgacgctcc gcagcatcgc cggcatcatt cccgccaccg
       61 caggcaccgt ccgcattgcg ggccacgatc tcgcgagcga tcccgtcgcc gccaaacgcg
      121 cgctcgcgtt ttttcccgac gaaccgcggc tcttcgaata cctcaccgtt cgtcagcacc
      181 tcgccttcgt cgcgaaaatc tacggtatcc acgatcacgc tacactcgcg caaccgctcc
      241 tcgatgaact cgagatcgcc gacaaagccg acgaccttcc cggtgcgctc tctcgcggca
      301 tgaagcaaaa actcgcgatc gcctgcggcc tcctgcacgc gccgtcggtg atgttcttcg
      361 acgaaccgct caccggcctc gatcccctcg gtattcgccg catgaaggat tcgattttga
      421 agcgcgcgcg cgccggcgcc gcgatcgtgc tcagctcgca cttgctacac ctgctcgaag
      481 aagtgtgctc gcatgtcttg atcttgaagc aaggcgcgaa gattgccgac ggcaccctcg
      541 ccgaagtcgc cgcgcgtttc agcaacggcg aacccgacgt gagcctcgaa gaaattttca
      601 ttagagcgac cggcggcacc gaaaactgat ctggaggaaa aaaacagatg aacaccgatg
      661 aacgcggatc gggcatgtcc tccgcacggc cacgccttca tccgtgttca tccgtgtcca
      721 tccgtggtta aatcatttca ccgcccgccg ccacccatgc tccgcgccct gctttatctg
      781 cgactcacct cgctgaagaa tctgctgctc acgcggctgc ggcgactgcg ccagcccaaa
      841 tacctcgccg gcgcattggt cggcggagcg tatttctact ttttcttttt ccgccacgcc
      901 cacacggcca cgcagaaaaa tcccggcgcg ttcttcacgc ttcccgccga cagcgccccg
      961 cttttcctcg gcgtcggagc cctcgcgctt ctcgcgatcc tgctcggcat gtggatcttt
     1021 ccttcgggca a
//
LOCUS       DASPLT010001270         1408 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_96196, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001270 DASPLT010000000
VERSION     DASPLT010001270.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1408)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1408)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1408
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_96196"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            303..974
                     /gene="cas5c"
                     /locus_tag="VEA63_12980"
     CDS             303..974
                     /gene="cas5c"
                     /locus_tag="VEA63_12980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007363023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-C CRISPR-associated protein Cas5c"
                     /protein_id="HYD84969.1"
                     /translation="MSYGIALRVSGPWACFTSPEFKTERRSYDVITPSAARGMLEAIH
                     WRPAIRWSIDRIHVLKPIRFQSLRRNEVGHKVPADTVRTVMRGASVRELRQIAGDDRQ
                     QRAATILVDVDYLVEAHITLTPQAGTGDSVAKHLAMFTRRARRGQCFHQPYLGTREFA
                     ADTELWDAPAPRHSLPDDQRDRDLGLMLLDVDYENDRASVFFHAVLRNGVMVVPPLPS
                     REVRS"
     gene            971..>1408
                     /locus_tag="VEA63_12985"
     CDS             971..>1408
                     /locus_tag="VEA63_12985"
                     /inference="COORDINATES: protein motif:HMM:NF021243.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-C CRISPR-associated protein Cas8c/Csd1"
                     /protein_id="HYD84970.1"
                     /translation="MTVLSSLNAHYARLERARDVAPFGFSTESISFALVLSADGDLLA
                     CRDLRDRAAQRPVGRPMTVPRSFKRPGTTPRAFFLWDNSKFVLGLGAAAAGGGLVRCA
                     GHAAAFRATHERLLDGATDRGLLAVLRFLRAWTPDRFDHAPVGS"
BASE COUNT          229 a          457 c          438 g          284 t
ORIGIN      
        1 ccgtgtgctg gtgcccgctc tcaaagagcg tcaatcgcgt gagcccagag caccggaagg
       61 aacatcagtg gatctctcgg cgtctggtgt ctatgaagtg gaaaacacaa aatgataggc
      121 cgtggtcacg caagggaagt gttttatgat gagtgaaatt gtatatagtg atatttctat
      181 aataaatgtt gatgctttat agagcgggat taatgtcgtg tttgattacc gcgtattcat
      241 ggcttgttgg ttcgttgaag gcggtgtctc ctggtgtgac caatagccac cggggtcatc
      301 tcatgtcgta tggcatcgca ttgcgcgtgt cagggccgtg ggcttgcttc acatccccag
      361 aattcaagac cgagcggcgg tcgtacgacg tgatcacgcc gtccgccgcg cgcgggatgt
      421 tggaggcgat tcactggcgg cccgcgatcc gctggtcgat cgaccgcatt cacgtgctga
      481 aacccatccg cttccagtcg ctccgccgca acgaggttgg tcacaaggtg cccgccgata
      541 ccgtgcgcac ggtcatgcgc ggcgcctccg tccgcgagct ccgacagatc gccggggacg
      601 atcggcagca acgcgccgcg acgatcctgg tcgatgtcga ctatctggtt gaagcgcaca
      661 tcaccctcac gccgcaggcg gggacgggcg acagcgttgc caagcacctt gccatgttca
      721 cccgccgcgc ccgccgtggt cagtgcttcc atcagcccta cctcggcacc cgcgaattcg
      781 ccgccgacac cgagctctgg gacgctccag cgccgcggca cagccttccg gacgatcagc
      841 gtgaccggga tctcggcctg atgctgctgg acgtcgatta tgagaacgac cgcgcctcgg
      901 tgttctttca cgccgtcctg cgcaacggcg tcatggtcgt gccgccgctg ccatcccgtg
      961 aggtgcggtc atgacggtcc tgtcatcgct caacgcccat tacgcgcgcc tcgaacgcgc
     1021 ccgtgacgtc gcaccgttcg gcttctcaac ggagagcatc tccttcgccc tcgtcctgtc
     1081 cgcggacggc gatctgctcg cctgccgcga cctgcgcgac cgggccgccc aacggccggt
     1141 cggccgtcca atgaccgtac cgcggtcgtt caagcggccc ggcacgacgc cgcgcgcctt
     1201 cttcctctgg gacaacagca agtttgtgct ggggctcgga gccgccgcgg ctggcggcgg
     1261 tctggtgaga tgcgcgggcc atgcggccgc cttccgcgcc acgcatgaac gcctgctgga
     1321 tggcgcgacc gatcgtggat tgctggcggt gcttcgtttt cttcgcgctt ggacaccgga
     1381 ccggttcgac cacgccccgg tcgggtcg
//
LOCUS       DASPLT010001271         1645 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_39171, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001271 DASPLT010000000
VERSION     DASPLT010001271.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1645)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1645)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1645
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_39171"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            180..536
                     /locus_tag="VEA63_12990"
     CDS             180..536
                     /locus_tag="VEA63_12990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD84971.1"
                     /translation="MLTLRIPSGRLPAALVALTASLFACFASSAHAQTYDYVVPLTQT
                     YVDGAVLGIQPGDTIGLEAGTRGRSARFPDVAELSGSHAGNFAGRADSCATRREKESV
                     VNLVQFQIRNHRDKLR"
     gene            587..796
                     /locus_tag="VEA63_12995"
     CDS             587..796
                     /locus_tag="VEA63_12995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD84972.1"
                     /translation="MRDPFPTLRREYLERHSDLATIEFYLCGPRPTIQAGCEILKTMN
                     APAANIATTNSPDVLPASRLEDRRS"
     gene            complement(793..1308)
                     /locus_tag="VEA63_13000"
     CDS             complement(793..1308)
                     /locus_tag="VEA63_13000"
                     /inference="COORDINATES: protein motif:HMM:NF022893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3016 domain-containing protein"
                     /protein_id="HYD84973.1"
                     /translation="MKTLGWIAVLLAAVGACSCASVQEGDGATVEVNYQAPEQFTDMS
                     MTYPGHGADEGYLQELRRHIERAAARQIPAGYTLALTITDVDMAGDFEPHRGPNLTDV
                     RIIKRIYPPRINLTYRLTDPSGAVRSEGERQLRNPAFDWNVSPIDREDSLRHEKALLD
                     DFLRDLAKEVR"
BASE COUNT          309 a          578 c          442 g          316 t
ORIGIN      
        1 ccggaggttg tgcgccaact gcaggcaagc acgtcgccag tggacaagtt cacgccttcg
       61 cactccgcgt gcgctcaacg tgtccaccct caaagtggtg ggagcgcagc ctcgcgttgg
      121 gctaacgctc ggcatcgttc cgccggcttc cccgccataa cccccaaccc cgtcctcgta
      181 tgcttacgtt acgaatcccc tccggtcggc tgcccgccgc actcgtcgca ctcacggcct
      241 ccctcttcgc ctgcttcgct tcctccgctc acgcccaaac ctacgattac gtcgttcctc
      301 tcacgcaaac ctacgtcgac ggcgccgtcc tcggcatcca gcccggcgac acgatcggtc
      361 tcgaggccgg cacgcgcggt cgtagcgcgc gtttccctga cgtcgcagag ctctcgggat
      421 ctcacgccgg taactttgca ggtagggcgg attcctgcgc gactcggcga gaaaaagaat
      481 ccgtcgtaaa cctcgtccag tttcagatcc gcaatcatcg cgacaaactc cgataacatt
      541 tcgagaaaat gcgagaagac catttttcgg agcgcggtta acctggatgc gagatccttt
      601 tcccacgctt cgtcgcgaat atctcgagcg tcactccgac ctcgccacca tcgagttcta
      661 cctgtgcggc ccgcgtccga cgatccaggc cggctgcgaa atattgaaga ccatgaacgc
      721 ccccgcagcc aacatcgcta cgacgaattc tcctgacgtt ctcccggcat cccgtctcga
      781 ggacagacgg agctagcgaa cctccttcgc caaatcgcga aggaaatcgt cgagcagcgc
      841 cttctcatgc ctcagcgagt cctcgcgatc gatcggcgat acgttccagt cgaatgccgg
      901 gttgcgcagt tgccgctctc cttcgctacg aacggctcca ctcggatcgg tgagtcggta
      961 agtgagatta atccgcggcg gataaatccg tttgatgatc cgcacgtccg tgagattcgg
     1021 tccacggtgc ggctcgaaat caccggccat gtccacatcg gtgatcgtca acgccagggt
     1081 gtagccggcg ggaatctgcc gggcggcggc acgctcgatg tgtcgccgga gctcctgcag
     1141 gtaaccttcg tccgcgccat ggcccggata cgtcatgctc atgtcggtga actgctcggg
     1201 cgcctggtag tttacctcga ccgttgcgcc gtcgccctcc tgcacgctcg cgcaggagca
     1261 cgcgcccacc gccgccagca gcactgcaat ccatccaagc gttttcatgc gacaacagtg
     1321 cacagaatct ctttcgcgca aacctcgcat catcggactt cttccctgta accctatccg
     1381 acgcgaccgc ggtcgcgtcg gcccgaagcg gaacgcaacg caagcgacgg cgaacgtcgc
     1441 ctcggagccc ggacctcctc ttgccgcgag cgctcgcact tcatcgccct ggcagcggcg
     1501 gaaaatattt ctgcaaaaag cgcaggcctt cgcgcgcgaa agtatcctgg tccggcgcgc
     1561 ggcgtttcca gatgcacacc gcaccggcga gatcgcgatt gtcgggagtg aaactctcga
     1621 tcaccaccgg cccccgataa tcgac
//
LOCUS       DASPLT010001272         1091 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_239222, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001272 DASPLT010000000
VERSION     DASPLT010001272.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1091)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1091)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1091
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_239222"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            116..>1091
                     /locus_tag="VEA63_13005"
     CDS             116..>1091
                     /locus_tag="VEA63_13005"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HYD84974.1"
                     /translation="MPNLWIANARIIDPAAKRDATGDLFISDGKLVDSLSAAAKKRAK
                     KIDAAGLIACPGLVDIHVHFREPGQTHKETIATGSRSAAAGGFTTVVCMPNTSPPADN
                     AGTIQFINDAIRRDAVVKVLPTGCITVGMKGQALAPIGSLKRTGVVAITDDGDCVQSN
                     ELMRRACEYAKMFDLPLMDHCQDASMTQGSVMNEGAMSTRLGLRGWPNAAEDLIVARN
                     VILSEYTGAHIHLQHISSRFSVEEIRRAKSRGARITAEATPHHIALTDDALATYDTNF
                     KMNPPLRTEDDRRALIDGLRDGTLDCISTDHAPHTDYEKDKEFDYAPNG"
BASE COUNT          226 a          427 c          266 g          172 t
ORIGIN      
        1 tctttctctt tcctctttat ctttctcttt cgtcagccct ccactcgacc gcgccgcaac
       61 cgacccccgg gagaaagata aagactaaag agaaagagaa agattctctc tcctcatgcc
      121 taatctctgg atcgccaacg cccgcatcat cgaccccgcc gccaagcgcg acgccacggg
      181 cgacctcttc atctccgacg gcaaactcgt cgactccctt tccgccgccg ccaaaaaacg
      241 cgcgaagaaa atcgacgccg ccggcctcat cgcctgcccc ggcttggtcg acattcacgt
      301 ccatttccgc gagcccggtc agacccacaa ggaaacgatc gccaccggct cccgctccgc
      361 tgccgccggc ggtttcacga ccgtcgtgtg catgcccaac acgtcgccgc ccgccgacaa
      421 cgccggcacc attcaattca tcaacgacgc cattcgtcgc gacgccgtcg taaaggttct
      481 ccccacaggc tgcatcaccg tcggcatgaa aggccaggcc ctcgcgccca tcggctcgct
      541 caaacgcacc ggcgtcgtcg cgatcaccga cgacggcgat tgcgtgcaga gcaacgaact
      601 catgcgccgc gcctgcgaat acgcgaagat gttcgatctt ccgctcatgg accattgcca
      661 ggacgcgtcc atgacccagg gctccgtcat gaacgaaggc gccatgtcca cccgcctcgg
      721 cctccgcggc tggcccaacg ccgccgagga ccttatcgta gcacgcaacg tcatcctctc
      781 cgaatacacc ggcgcgcaca tccacctgca gcacatctcc tcgcgcttct ccgtcgaaga
      841 gatccgccgc gcgaaatccc gcggcgcccg catcaccgcc gaggcaacgc cgcaccacat
      901 cgcgctgacg gacgacgccc tcgcgaccta cgacacgaat ttcaagatga acccgccgct
      961 gcgcaccgag gacgaccgcc gcgcgctcat cgacggcctg cgcgacggca cgctcgattg
     1021 catcagcacc gaccacgcgc ctcacaccga ttacgagaag gacaaggagt tcgactacgc
     1081 gcctaacggt a
//
LOCUS       DASPLT010001273         1306 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_302456, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001273 DASPLT010000000
VERSION     DASPLT010001273.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1306)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1306)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1306
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_302456"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1249
                     /locus_tag="VEA63_13010"
     CDS             <1..1249
                     /locus_tag="VEA63_13010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HYD84975.1"
                     /translation="KTLQKNTTLNRGPSTFWAKAGIARTMNTLAQEAWGSWSPDGTQA
                     APEALVNKWFFGSSYDAAIASQPLPANWRDQIGTLVAQPLRIRSGAVPGSGNYVAQGT
                     IDPNTSSPYLAPPLTADEVEYRRAWFAARSDAEWFRPLNPAWVAAEQFEKITGDDYRI
                     WGESPPAGQKLTNDLVSKGVEIELTANPLPNWRLTLNAAEIKAQRSNILPDWAPFVAE
                     NRDLWFEGYNNNPGGPSQLNYWTIDGFADLRHWGGTTAYSSAADTFGGRMMQDVYGPY
                     VNAIAGEGVSVSELRKWRVNFVTNYTFSSGTLKNVNVGGAVRWQDKTAIGYLPQYNEE
                     ADIWVTNVSNPYFGPSETNYDFWVGYRRALSDKVTWEIQLNVRDLFADDDLVPIQANP
                     DGTIAQVRIPSETTWMITNTFRF"
BASE COUNT          270 a          448 c          386 g          202 t
ORIGIN      
        1 caaaacgctt cagaaaaaca ccacgctgaa ccgcggcccc tcaaccttct gggccaaagc
       61 cggcatcgcg cgcacgatga acacgctcgc ccaggaggcg tggggatcat ggagcccgga
      121 cggcacgcag gccgcgcccg aagcgctcgt caacaaatgg ttcttcggca gttcctatga
      181 cgccgccatc gccagccaac cgctgcccgc aaactggcgc gatcagatcg gcacgctcgt
      241 cgcgcagccg ctgcgcattc gcagcggagc cgttccagga tcgggcaact acgtggcgca
      301 gggaaccatc gatccgaaca ccagctcgcc ctatctcgct ccgccgctca ctgccgacga
      361 ggtcgagtat cgccgcgctt ggttcgccgc gcgcagcgac gccgaatggt tccgtccgct
      421 taatcctgca tgggtggctg ccgaacaatt cgaaaaaatc accggcgacg actaccgcat
      481 ctggggcgaa agcccgcccg ccgggcaaaa gctcaccaac gatctcgtgt cgaagggcgt
      541 cgagatcgag ctcaccgcca acccactccc caactggcgc ctcacgctca acgccgccga
      601 aatcaaagcg caacgcagca acatccttcc cgattgggcg cccttcgtcg cggagaaccg
      661 cgatctctgg ttcgaaggct acaacaacaa ccccggcggt ccgagccagt tgaactactg
      721 gacgatcgac ggcttcgccg acctgcggca ctggggcggg accaccgcgt actcctccgc
      781 ggccgatacc tttggcggcc gcatgatgca ggacgtctac gggccgtacg tgaacgccat
      841 cgccggcgaa ggcgtctccg tcagcgaact acggaagtgg cgcgtcaact tcgtcacgaa
      901 ctacacgttc agcagcggaa cgctgaagaa cgtcaatgtc ggcggcgccg tgcgctggca
      961 ggacaagacg gccatcggct acctgccgca gtacaacgag gaggccgata tctgggtcac
     1021 gaatgtcagc aacccgtact tcgggccgag cgagacgaac tacgacttct gggtcgggta
     1081 ccggcgcgcg ctctcggaca aggtgacgtg ggagattcag ctcaacgttc gcgacctgtt
     1141 cgccgatgat gatctggttc cgatccaggc caatccggac ggcacgatcg cccaggtacg
     1201 gattccaagc gagacgacct ggatgatcac caatacgttc cggttctgaa gctcgcgtgg
     1261 ccggccttct ggaacggctg gccggcgcag tcccgcctcc atgggc
//
LOCUS       DASPLT010001274         1215 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_219523, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001274 DASPLT010000000
VERSION     DASPLT010001274.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1215)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1215)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1215
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_219523"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..510
                     /locus_tag="VEA63_13015"
     CDS             <1..510
                     /locus_tag="VEA63_13015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD84976.1"
                     /translation="CALVALIGLGGLSGCRTAKTASFRFVDPARLDAPIDASKVKIEE
                     QTVIFIDATPIGTLATPDFPDGAWALPKSSLTVVVRVAVNEKGRAMVVGKSIGDLSLS
                     TPFTAACEEAIHRAVATWRFEPAQLGTLEPQEDGRPLLVSSSVTDTMLEVAFTFSPSG
                     RVGATVDRK"
     gene            611..1060
                     /locus_tag="VEA63_13020"
     CDS             611..1060
                     /locus_tag="VEA63_13020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD84977.1"
                     /translation="MKPIFSRVGLILLAGIFIVSGCNTPQRFEDVSSSLAYRAYVGAR
                     YELQVAMHLSGVNAPPGYEKTADYYVIDSVSPSWSGPELITRDVLAPGTEIIVDSVRR
                     CINCPFDEIVSAGVRISGEPTPLGRPIYVQLKYLAPDFAKKQEGPPQ"
BASE COUNT          237 a          325 c          396 g          257 t
ORIGIN      
        1 tgcgcgctgg tggctttgat tggtctcggc ggtctgagcg gatgccgcac ggcgaagacg
       61 gcctccttta ggtttgttga tccggcaaga ttggatgcgc cgatcgatgc cagtaaggtg
      121 aagatcgagg agcaaaccgt gattttcatc gatgcaacgc cgatcgggac gctcgcgacg
      181 ccggactttc ctgacggcgc gtgggcattg ccgaagtcat cgctcaccgt cgtggttcga
      241 gtcgcggtta acgagaaggg cagagcgatg gttgtcggga aaagcatcgg agatctctcg
      301 ctttcgacgc cgttcacggc ggcgtgcgag gaagctattc acagagctgt agcgacatgg
      361 cggttcgaac ccgcccaact cggcacgctc gagccgcagg aagacggtcg gccgctgctg
      421 gtgtcgagca gcgtcaccga tacgatgttg gaggtcgcgt ttacgtttag tccttccggg
      481 cgagttggcg cgacggtcga tcggaagtga aaacggaaca gccgctacgg cgatgccgac
      541 attcgacgcg tcggtgccgg ggtctctttt cgtgtgagtt gccgcgggaa cttatcaatt
      601 attcggtgaa atgaaaccca tcttctcgcg agtcggctta atattgttgg ccggaatttt
      661 catcgtttcc ggctgtaaca cgccgcagcg atttgaggac gtgagttcca gcctggccta
      721 tcgcgcctac gtaggcgccc ggtatgagct gcaagttgcc atgcacctct ccggcgtgaa
      781 tgcgcctccg ggatacgaga agacggccga ctactatgtc atcgattcag tgtcgccgag
      841 ttggagtggc ccggagttga tcacgcgcga cgtgctcgct ccggggacgg aaatcatcgt
      901 ggactcagtg cgccgatgca taaattgccc attcgatgaa atcgtcagcg cgggggtccg
      961 catctctggc gagccaacgc cgcttggccg tccgatctac gtccaattga aatatctcgc
     1021 gccggatttc gccaagaagc aggaaggacc gccccagtag gaatgaaaag cgtgaggcac
     1081 cggcgcggaa tggcaggcgt caatgcgcgc ctaggcacga gcacagctac ggttggttgg
     1141 atcttgggaa cggcggcgtt gtgcgtcatc ggcaatcgcc gaggacgatc ctgtagatga
     1201 ttgtttatcg gcggc
//
LOCUS       DASPLT010001275         2884 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_39201, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001275 DASPLT010000000
VERSION     DASPLT010001275.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 2884)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2884)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2884
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_39201"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(175..501)
                     /locus_tag="VEA63_13025"
     CDS             complement(175..501)
                     /locus_tag="VEA63_13025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD84978.1"
                     /translation="MRTLTFIVLLAVLSAPFIAWAETSDSRVNRLETRVRKLERSGRS
                     VVGIAFVAGVVAALWAQNTRRNAWLWFFAGVIFAPITLLVLLYLNAQDRDRDRDPPRR
                     MNDRAG"
     gene            complement(587..1105)
                     /locus_tag="VEA63_13030"
     CDS             complement(587..1105)
                     /locus_tag="VEA63_13030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD84979.1"
                     /translation="MNPTLRGLVAWIGMRAAGLATVGLGARGVNAAAEERPPIFAAKK
                     LEVVETDAGDKRVPLLSATAKRRIAEQVLADVRASAEAKAGEVAVEKAATELGAEGDV
                     VVLERYVVRELRGPELVAPKNRALEMIRTGRLHTSDSGKTSIDLLGSSVWNSSVGDTG
                     DAKLTLKFTFRW"
     gene            complement(1248..>2884)
                     /locus_tag="VEA63_13035"
     CDS             complement(1248..>2884)
                     /locus_tag="VEA63_13035"
                     /inference="COORDINATES: protein motif:HMM:NF017745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF885 domain-containing protein"
                     /protein_id="HYD84980.1"
                     /translation="TECIESLLEARPETATQLGEHRFDDRLTDYSAAALAAREAALRA
                     QIAALEKIDAAALTGANRIDAQILRLELGSMLFVLTEEKPHTWNPLPYNDSLANSIYL
                     LTAREFAPAEMRLRSAAKRLEAIPRVVAQIKATLKNPPRIHTETAIQQTAGAIALVRE
                     ELEPLLAQAPSMREELAPAQAVAVAALEDYKAWLEREVLPKANGEFRLGEEKFRKKLR
                     FALDSELAPDEILRRAERELETTTKAMYATARPLFENYFPAASTAEREDRPRVIKAVL
                     DRLAEQRPTDATVVARATEITEAATAFVREKNLVTVPETPLKIIVLPEFQRGVAIAYC
                     DSPGALESEGETYFAVSPTPANWTEARRVSFFREYNDYMLHDLAVHEAMPGHYLQIAH
                     ANAFRAPTLVRAVFASGTFIEGWAVYAEHVMADAGFGGPEVKLQQLKMRLRAIINAIL
                     DNKVHTAGMTEKEALDMMIRRGFQEEGEAVGKWRRACQSSTQLSTYFVGAAEHDDMRA
                     AAERKEGTAFDLKRYHDRVLSFGSPAVKYVRQELGW"
BASE COUNT          502 a         1157 c          699 g          526 t
ORIGIN      
        1 tgctgagcgc cccagacaac accaccgcga tcgcgacgcg cccaacgccc gctttgaatt
       61 ttcgccgcat accgctttct cccatcgccg actcgctacg gaccgccacc gtccgcgtca
      121 acgccctcgt cgcaacacgc atccgtgcga gcgctgcatt cgcgtcttca gcgtttaccc
      181 cgcgcgatcg ttcatcctgc gaggcggatc gcggtcgcgg tcccgatcct gcgcattgag
      241 gtagagcaac accagcaacg tgatcggcgc gaagatcact ccggcaaaaa accacagcca
      301 cgcgtttctc cgcgtgtttt gcgcccacaa cgccgccacc acgcccgcga caaatgcgat
      361 gcccaccacc gaccgaccgc tccgctccaa ttttcttacc cgcgtttcca accggttcac
      421 gcgcgagtcg ctcgtctccg cccatgcgat aaacggagcg gacagcactg cgagcagcac
      481 gatgaacgtc agggtcctca tagaatttca cccgccgccg gggcgcgcga tcaaagtcgt
      541 ttctctcgcc gcaaagcaat cgccgattcg ccacgctgtc gcgaactcac caccgaaacg
      601 taaacttcaa cgtcagcttc gcatcgccgg tatcgcccac gctcgagttc cacacgctcg
      661 atcccagcag gtcgatcgac gtcttgcccg aatcgctggt gtgaagtctt cccgtgcgaa
      721 tcatctccag cgcccggttc ttcggtgcca ccaattccgg tccgcgcagc tcgcgcacca
      781 catagcgctc gagcaccacg acgtcgcctt ccgcgcctaa ctccgtcgcc gctttctcca
      841 ccgcgacctc gcccgccttc gcctccgcac tcgcgcgcac gtcggccaac acctgctccg
      901 caatccgccg cttcgccgtc gccgacaaca acggcacccg cttatcgccc gcgtccgttt
      961 caaccacctc cagcttcttc gccgcaaaaa tcggcggccg ctcctccgcc gccgcgttca
     1021 cgccccgcgc tccaagtccg accgtcgcca gccccgctgc ccgcatcccg atccaggcaa
     1081 ccaggccgcg caaggtgggg ttcatgagct tgccatgtga aacataactt cagcgccggt
     1141 tccctgcaga ttgccgccac acccaaactc cccaacccct ggagccgcgg cgcccccgcc
     1201 gcggtgtctt cataatctaa tccacaatct ccaccgcgtc gcgtccctca ccaccccaat
     1261 tcctgccgca cgtatttcac cgccggactc ccgaacgaca gcacgcgatc gtgataacgc
     1321 ttcagatcga acgccgtgcc ttcctttcgc tccgccgccg cacgcatgtc gtcgtgctcc
     1381 gccgcaccga caaaatacgt cgaaagttgc gtcgagctct gacacgcccg ccgccatttc
     1441 cccaccgcct cgccctcttc ttgaaacccg cgccggatca tcatatccag cgcttccttc
     1501 tccgtcattc ccgccgtgtg caccttgtta tcgaggatcg cattgatgat cgcccgcagc
     1561 cgcatcttga gctgctgcag cttcacctcc ggcccgccaa aacccgcgtc cgccatcacg
     1621 tgctccgcgt aaaccgccca cccttcgatg aacgtcccgc tcgcgaacac cgcgcgcacc
     1681 agtgtcggcg cgcgaaacgc attcgcatgc gcgatctgca gataatgccc gggcatcgcc
     1741 tcgtgcaccg cgaggtcgtg cagcatgtag tcgttgtatt cacgaaaaaa cgacacccgc
     1801 cgcgcctccg tccagttcgc cggcgtcggc gacaccgcga aatacgtctc accctccgac
     1861 tccagcgcgc ccggcgaatc gcaatacgcg atcgccacgc cgcgctgaaa ctccggcagc
     1921 acgatgatct tcaacggcgt ctccggcacc gtcacgagat tcttctcccg cacgaacgcc
     1981 gtcgccgctt ccgtgatctc cgtcgcccgc gcgaccaccg tcgcgtccgt cggacgctgc
     2041 tccgccagcc ggtcgagcac cgccttgatc acgcgcggcc gatcctcgcg ctctgccgtg
     2101 ctcgccgccg ggaaatagtt ttcgaacaac ggccgcgccg tcgcatacat cgcctttgtc
     2161 gtcgtttcca actcccgctc cgcacgccgc aaaatttcat ccggcgccaa ttcggaatcc
     2221 aacgcgaacc gcagcttctt ccgaaacttc tcctcgccca accgaaactc gccattcgcc
     2281 ttcggcagca cttcacgttc aagccacgcc ttgtaatcct ccaacgccgc caccgccacg
     2341 gcttgcgccg gtgcgagctc ctcgcgcatc gacggcgcct gcgccagcag cggttcaagc
     2401 tcttcccgca ccagcgcgat cgctcccgcc gtttgctgaa tcgccgtttc ggtgtgaatc
     2461 cgcggcggat ttttcagcgt cgccttgatc tgcgcgacga cccgcggaat cgcctccaac
     2521 cgtttcgccg cgctccgcaa ccgcatctcc gccggcgcga actcccgcgc cgtcagcaga
     2581 taaatgctgt tcgccaggct gtcgttatat ggcagcggat tccacgtatg tggtttctcc
     2641 tcggtcaaaa cgaagagcat tgaacccagc tcgagccgca aaatctgcgc gtcgatccgg
     2701 ttcgcacccg tcaacgccgc cgcatcgatc ttctccagcg ccgcgatctg cgcccgtaac
     2761 gccgcctcac gcgccgccag cgccgccgcg ctgtagtcgg tcagccggtc gtcgaaacga
     2821 tgctcgccca gctgcgtcgc cgtctccggc cgcgcttcga gcaaactctc gatgcactcc
     2881 gtcg
//
LOCUS       DASPLT010001276         1988 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_219567, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001276 DASPLT010000000
VERSION     DASPLT010001276.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1988)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1988)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1988
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_219567"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..437
                     /locus_tag="VEA63_13040"
     CDS             <1..437
                     /locus_tag="VEA63_13040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase"
                     /protein_id="HYD84981.1"
                     /translation="PLELDSPVCQLVVHHLVHHHTTGEPYSDTQVRRLARKLAPATID
                     DLALVMTADSFGRPPLDSPQTLELIAALRAKAHALELERAAPRPLIQGRHLIALGHKP
                     GPSFKPVLEAAFEAQLDGVFVDEAGGREWLQSYLQRAPSGLT"
     gene            452..1399
                     /gene="tal"
                     /locus_tag="VEA63_13045"
     CDS             452..1399
                     /gene="tal"
                     /locus_tag="VEA63_13045"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HYD84982.1"
                     /translation="MPSSQLEQLKQFTKVVADTGDFSSIKEYKPQDATTNPSLILKAA
                     GMPDYAHLVDQAVKDAGSGASIDTVIDLLLVLFGREILKIVPGRVSTEVDARLSFDRD
                     GSIAKAREIIAFYEKAGIPRERVLIKLASTWEGIKAAEQLQQEKINCNLTLLFSFGQA
                     VACADAKVQLISPFVGRILDWYKKSTGRDYAPADDPGVKSVAQIYTYYKKFGYKTEVM
                     GASFRSKGEVLELAGCDLLTISPQLLGELAASNDPVTRKLDPAAAASAQIDRVNLDEK
                     AFRWELNEDQMATEKLSEGIRQFAADIVKLETLIKQKRA"
     gene            1500..>1988
                     /locus_tag="VEA63_13050"
     CDS             1500..>1988
                     /locus_tag="VEA63_13050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007362085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 20 glycosylhydrolase"
                     /protein_id="HYD84983.1"
                     /translation="MIRSFQWDLARQVERLDWLIAQLPRYADWGYHELHLHLEDAVEY
                     PSLPGVARRDAYTYKQFARLVHAATHAGIKVVPIVNLLGHTQYLIKVPELRDLNELRA
                     ADGSPLERGQICPLHPRTLEIAEKLLRDIAPFCTAGKVHVGLDESFHLGRHPLSRREI
                     AKI"
BASE COUNT          412 a          693 c          532 g          351 t
ORIGIN      
        1 cgccgctcga gctcgactcg cccgtctgcc agctcgtcgt gcatcatctc gttcaccatc
       61 acacgaccgg cgagccttac agcgacacgc aggtccgccg gctcgcgcgc aaactcgctc
      121 cggcaaccat cgacgatctc gcgctcgtga tgaccgcgga ctccttcggt cgtccgccgc
      181 tcgactcacc acagacgctc gagctcatcg ctgcgctccg cgcgaaagcc cacgcgctcg
      241 aactcgagcg cgccgcgccg cggccgttga tccaaggccg tcacctcatc gcactcggcc
      301 ataaacccgg accaagtttc aagccggtgc tcgaggctgc tttcgaagct caactcgacg
      361 gcgtatttgt cgacgaggcc ggcggtcgcg aatggttgca gtcgtatttg caacgcgctc
      421 cgtccggtct aacgtgaacc cgtcttttat catgccctcc tcccaactcg agcaactcaa
      481 gcagttcacc aaagtcgtcg ccgacacggg cgatttttcc agcatcaagg aatacaagcc
      541 gcaggacgcg acgacgaatc cgagccttat cctcaaagcc gccggcatgc cggactacgc
      601 ccatctggtc gaccaagccg tgaaagatgc cggctccggc gcatcgatcg acaccgtgat
      661 cgacttgctg ctcgtgttgt tcggtcgcga gattttgaaa atcgtgccgg gacgtgtctc
      721 gacggaagtc gacgcgcggc tttcgttcga ccgcgacggc agcatcgcaa aggcgcgcga
      781 aatcatcgcc ttctacgaga aagccggcat cccgcgtgaa cgcgttctca tcaaactcgc
      841 ctcgacttgg gaaggcatca aagccgccga gcagctccag caggaaaaaa tcaattgcaa
      901 cctcacgctg ctcttctcct tcgggcaggc cgtcgcgtgc gccgacgcga aggtgcagct
      961 catctcgccg ttcgtcggac gcattctcga ttggtataaa aagagcaccg gccgcgacta
     1021 cgcccccgcc gacgatccgg gcgtgaaatc cgtcgcgcag atttacacct attacaaaaa
     1081 attcggctat aaaaccgaag tcatgggcgc gagcttccgc agcaaaggcg aagtgctcga
     1141 actcgccggc tgcgatctcc tgacgatctc gccgcaactg cttggtgaac tcgccgcttc
     1201 caacgatccg gtcacgcgca aactcgatcc cgccgccgcg gcgagcgcgc agatcgatcg
     1261 cgtaaacctc gacgaaaaag catttcgctg ggagctcaat gaagatcaga tggcgacgga
     1321 aaaactctcc gaaggcatcc gccagttcgc cgccgacatc gtgaagctcg aaacgctcat
     1381 caaacaaaag cgcgcttaac ctcgtagggc cgggctttag cccgcccaga ttggaaaagc
     1441 gggttgaaac ccgccctact tgcgccgcgc gcctgcggcc gtcatcactg cgagcgcaca
     1501 tgattcgctc gttccaatgg gacctcgcgc gccaagtcga acgcctcgac tggctcatcg
     1561 cgcagcttcc ccgctacgcc gattggggtt atcacgaact gcatcttcat ctggaagacg
     1621 ccgtcgagta tccgagcctg cccggcgttg cgcgccgcga cgcctacact tacaagcagt
     1681 tcgcgcggct cgttcacgcc gccacgcacg ccggcatcaa ggtcgtgccc atcgtcaacc
     1741 tcctcgggca cacgcaatat ctcatcaagg tgcccgagct gcgcgacctc aacgaactcc
     1801 gcgccgcgga tggatcgccg ctcgaacgcg gacaaatttg tccgctgcat ccgcgcacgc
     1861 tcgagatcgc agaaaaactc ctgcgcgaca tcgcgccatt ctgcaccgcg ggaaaagttc
     1921 acgtcggcct cgacgaatcg tttcacctcg gccggcaccc gctgagccgc cgcgagatcg
     1981 ccaagatc
//
LOCUS       DASPLT010001277         1124 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_219574, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001277 DASPLT010000000
VERSION     DASPLT010001277.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1124)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1124)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1124
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_219574"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            184..>1124
                     /locus_tag="VEA63_13055"
     CDS             184..>1124
                     /locus_tag="VEA63_13055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012377109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HYD84984.1"
                     /translation="MMEQRVTLTQIAQRAGVHVTTVSLAMRNHPRLPESTRQRIQALA
                     REMGYAPDPFLRALVAYRGEHARRRKPPVLAYVTNWHTCWGWKDVFAHRKFFAGAEAK
                     ALELGFKLEHFWLHEPEHAEKRLGRALRERGINGVILASHSRDMGDELQLDWDHFSAV
                     KIDYFPHRPPLHNVTNSQSRIIRLAVRKTVAAGYRRIGFVVHRGWDHAVDHNWTAGFL
                     CEQHLLPEEDRVPSHVFPPPHPVERWFNETNASICADREPLERWLNEHKPDVVIAKAA
                     YVLPAFQQMGLSFPRDIAYVNPFLEEPDGTIAGVRQN"
BASE COUNT          204 a          336 c          382 g          202 t
ORIGIN      
        1 acctgtccga aacgcggagc gccgcccgcc gtgccggtgg agattgcgcc gtcgcgcggg
       61 gaatagcggc ggcttcgagc gagcgtcgac gagcggcggc agggcgattt gaaaacggcg
      121 ctcgcggcgc cggtctaaag acttgtgccc tgcgaggaaa cgcgcgctag atgaggcgcc
      181 gccatgatgg aacaacgggt caccctcacc cagattgccc aacgtgcggg tgtgcacgtg
      241 acgaccgttt ctttggcgat gcgaaatcat ccgcgtttgc cggagagcac acgtcaaaga
      301 attcaggcgc tcgcgcgcga gatgggatac gcgccggatc catttttgcg cgcgctcgtg
      361 gcgtatcgcg gtgaacacgc gcgacggcgg aagccgccgg tcctcgccta cgtcacgaac
      421 tggcacacgt gctggggctg gaaggatgtt ttcgcgcatc gaaaattttt cgcgggcgcg
      481 gaggcgaagg cgctcgagct gggtttcaag ctcgaacatt tctggctgca cgagcctgag
      541 cacgcggaga aacggctcgg gcgcgcgttg cgcgagcgcg gcatcaacgg tgtgattctc
      601 gcgtcgcaca gccgcgacat gggcgacgag ttgcagctcg actgggatca tttcagcgcg
      661 gtgaagatcg actacttccc gcaccggccg ccgctgcaca atgtcaccaa ctcgcaatcg
      721 cgcatcatcc ggctggcggt gcgcaagacg gtcgccgcgg gttatcggcg catcggtttc
      781 gtcgtgcatc gcgggtggga tcatgcggtc gatcacaact ggacagcggg gtttctgtgc
      841 gaacagcatc ttttgccgga ggaggatcgc gtgccgtcgc acgtgtttcc tccgccgcat
      901 ccggtcgaac gctggttcaa cgagaccaat gcgtcgatct gcgccgaccg ggagccgctc
      961 gagcgctggt tgaacgagca caagcccgac gtggtgatcg cgaaagcggc gtatgtcttg
     1021 cccgcgtttc aacagatggg gctgagtttc ccgcgcgaca tcgcgtatgt taacccgttt
     1081 ctcgaggagc ccgacggaac gatcgcgggc gtgcgccaga atca
//
LOCUS       DASPLT010001278         1739 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_15765, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001278 DASPLT010000000
VERSION     DASPLT010001278.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1739)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1739)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1739
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_15765"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..192
                     /locus_tag="VEA63_13060"
     CDS             <1..192
                     /locus_tag="VEA63_13060"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate binding protein"
                     /protein_id="HYD84985.1"
                     /translation="DHHDAGRETGLIAARVLRGENPADIPYRSSTLTRLIVNLRLARE
                     AGLTLPPAVLARADQIVEN"
     gene            378..758
                     /locus_tag="VEA63_13065"
     CDS             378..758
                     /locus_tag="VEA63_13065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD84986.1"
                     /translation="MPPTSAAAPALEHICVSLRRSSATFDGAAFVGTGRAMIDALATH
                     LQRPPLDILDRLVKGLLVNKGAHWTSTIVGEYLHLYAPSAGNFCVVAEREAGLVRRAR
                     IFARDKRPRVSRSQRPRTRLRPRI"
     gene            755..1678
                     /locus_tag="VEA63_13070"
     CDS             755..1678
                     /locus_tag="VEA63_13070"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HYD84987.1"
                     /translation="MKNPSLETPPAAGTRWWFIYAAIAAIAWGVWGAVIDTTARAGFP
                     AEMGYVVWSFTMVPPALFALTRAGWRLERNAAAIALGGAAGLLGAGGQLILFKVLRLA
                     PAYLVFPIIALSPIITVALAFLALRERVAIKDWIGILLALGAGVLLAYSPSGDGSVSR
                     GWLALTVLVFVCWGVQGFVISRANRVMRAESVFFYMMLTGLLLAPVALAMVDFAQPIN
                     WGLRGLWTAAAIQSLNAIGALLLVYALRYGKAMVVAPLINAGAPMITILLSLLLYQTL
                     PNAQNAWGMAIALVATVLLAWESAPGLQKPR"
BASE COUNT          258 a          619 c          514 g          348 t
ORIGIN      
        1 gatcatcacg acgccggccg tgagacgggc ctcatcgcgg cgcgcgtgct gcgcggcgaa
       61 aaccccgccg acattcccta tcgttcctcc acgctcactc gtttgatcgt caatctccgg
      121 ctcgctcgcg aagccggcct caccctcccg cccgcagtcc tcgcccgcgc cgatcaaatc
      181 gtcgagaact gacccgccgc tagccgcatc acgtccgcca gggtgcgcga gtatttccgc
      241 ctcaaattcg atctaagctc ctccgaccag caggcgcacg cattcatgtc gtgtggatcc
      301 gcggtgagtc gcggttgaac gggttctctg ccggctcgtt ttccgagagc tcgctgttcg
      361 cgtaaggaga tcggcgcatg ccacccactt ccgcggcggc tcccgccctg gagcacattt
      421 gcgtctccct ccgccgctcg agcgccacgt tcgatggcgc cgcatttgtc ggcactggac
      481 gcgccatgat cgatgcattg gccactcacc tccagcgtcc gcccctcgac atcctcgaca
      541 ggttggtcaa aggcctcctc gtcaacaaag gcgcccactg gacgagcacg attgtcggcg
      601 aatacctgca cttgtatgcg ccgtcggccg gaaacttctg cgtcgtcgcc gagcgcgaag
      661 ctggccttgt tcgccgcgct cggattttcg cgcgggacaa acgtccacgc gtttcccggt
      721 cgcaacgacc ccgcactcgc ctgcgtccac gaatatgaaa aatccctcgc ttgaaactcc
      781 acccgctgcg gggactcgct ggtggttcat atatgccgcg atcgccgcca tcgcttgggg
      841 cgtctggggc gcggtgatcg acacgacggc gcgcgccggt ttccccgccg aaatgggcta
      901 cgtcgtgtgg tcgttcacca tggtgccgcc ggccctgttt gcgctgacac gcgccggctg
      961 gcggctggaa cgaaacgccg cggcgatcgc cctcggaggc gccgccggcc tgctcggcgc
     1021 cggcgggcag ttgatcctct tcaaggtcct ccggctcgcc ccggcgtatc tcgtttttcc
     1081 gatcatcgcc ctctcgccga tcatcaccgt cgcgctcgca ttcctggcct tgcgcgagcg
     1141 cgtcgccatc aaggattgga tcggcattct gctggctctc ggcgcgggag tgctgctcgc
     1201 gtattcgcct tctggagacg gcagcgtctc gcgcggctgg cttgcgctca ccgtgctcgt
     1261 gttcgtctgc tggggcgtgc aggggttcgt gatctcacgc gcgaaccgcg tcatgcgcgc
     1321 cgagagcgtg ttcttctaca tgatgctgac ggggctcctc ctcgcgcccg ttgcgctcgc
     1381 catggtggat ttcgctcagc cgatcaactg gggcttgcgc ggcttgtgga cggcagccgc
     1441 gatccagagc ctcaacgcca tcggtgcgct gctcctcgtc tacgcgctgc gttacggcaa
     1501 ggcgatggtc gtcgccccgc tgatcaacgc cggcgctccg atgatcacga tcctcctctc
     1561 gctcctcctt taccaaacgc tcccgaacgc gcaaaacgcc tggggcatgg cgatcgcgct
     1621 ggtggctacc gtcctcctcg cgtgggaaag cgccccgggc ctccagaagc cgcggtgaac
     1681 ggaaaaaatt atctcagctg cagacgcgaa acgtcattaa tgaattctgc ctccagaga
//
LOCUS       DASPLT010001279         1246 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_179909, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001279 DASPLT010000000
VERSION     DASPLT010001279.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1246)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1246)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1246
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_179909"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1246)
                     /locus_tag="VEA63_13075"
     CDS             complement(<1..>1246)
                     /locus_tag="VEA63_13075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HYD84988.1"
                     /translation="NSQPSTLNSTCADQVRLAIADACKRLLCPAMETEMRLESKKRAD
                     EAGIKVFADNLRELLLAAPLGQKAVMAIDPGIRTGCKTVLLDRQGKLLHNDVVFPDRL
                     EADAKEKLSGFVKFFNVEAIAIGNGTAGRETEAFVRKLGLPATIPIVMVNESGASIYS
                     ASEVAREEFPDHDLTVRGAVSIGRRLMDPLAELVKLDPKSIGVGQYQHDVDQLALKRS
                     LDDTVVSSVNGVGVELNTASKQLLSYVSGLNATTAAAIVARRNEKGAFKSRAELKEVP
                     RLGPKAFEQAAGFLRIRDAAHPLDSSAVHPERYALVEKMAADLGATVADLVRDGKLRA
                     KIKLENYVTADVGLPTLTDIMAELAKPGRDPREKFEAFSFDAGVHDPKDLKPGMKLPG
                     IVTNVTAFGAFVDIGVHQDGLVH"
BASE COUNT          204 a          324 c          464 g          254 t
ORIGIN      
        1 gtgcacgagg ccgtcctggt gcacgccgat gtcgacgaac gcgccgaagg cagtgacgtt
       61 cgtgacgatg cctggaagct tcatgccggg cttcaagtct ttcgggtcgt gcacgccggc
      121 atcgaaggaa aacgcttcga acttttcgcg cgggtcgcgg ccgggtttcg cgagctcagc
      181 catgatatcg gtgagcgtgg gcaggccgac gtcggcggtg acatagtttt cgagtttgat
      241 cttcgcgcgg agttttccgt cgcgaacgag atcggcgacg gtcgcgccga gatcggcggc
      301 cattttctcg acgagcgcgt aacgttcggg gtggacggcg ctggaatcga gcgggtgcgc
      361 ggcgtcgcgg atgcggagaa agccggcggc ttgctcgaac gctttcgggc ctagacgcgg
      421 aacttccttc aattcggcgc gggatttgaa cgcgcccttt tcgttgcggc gcgcgacgat
      481 cgcggcggcg gtggtggcgt tgaggccgga gacgtagctg agaagttgct tcgaagcggt
      541 gttgagttcg acgccgacgc cgttgacgga gctgacgacg gtgtcgtcga gcgagcgttt
      601 gagcgcgagc tggtcgacgt cgtgttgata ctggccgacg ccgatcgatt tcggatcgag
      661 cttcacgagc tcggcgagcg gatccatcag tctccgtccg atggatacag cgccgcggac
      721 ggtgagatcg tgatcgggga attcttcgcg cgcgacctcg ctggcggagt aaatcgaggc
      781 gccggattcg ttgaccatca cgatcggaat cgtggcgggc aggccaagtt tgcggacgaa
      841 ggcttcggtt tcgcggccgg cggtgccgtt gccgatggcg atggcctcga cattgaagaa
      901 cttcacaaaa ccggaaagtt tctcttttgc gtcggcttcg aggcggtcgg gaaaaacgac
      961 gtcgttgtgg aggagcttgc cctgacgatc gaggagcacg gtcttgcagc cggtgcggat
     1021 gccggggtcg atggccatga cggctttctg gccgagcggc gcggcgagga ggagctcgcg
     1081 gagattgtcg gcgaagactt tgatgccggc ttcgtcggcg cgctttttcg attcgaggcg
     1141 catctcggtt tccatcgcgg ggcaaagcag gcgtttgcac gcgtcggcga tggcgaggcg
     1201 gacctgatcg gcgcaggtgg agttgagagt tgagggctga gagttg
//
LOCUS       DASPLT010001280         5981 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_96335, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001280 DASPLT010000000
VERSION     DASPLT010001280.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 5981)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5981)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5981
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_96335"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1220
                     /gene="nuoH"
                     /locus_tag="VEA63_13080"
     CDS             <1..1220
                     /gene="nuoH"
                     /locus_tag="VEA63_13080"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009509697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HYD84989.1"
                     /translation="APSNFLEHAPLAARDWLVSIFPAPLHWLVHHLITIIAILAVFGL
                     FFALLTVVERKLLARIQNRLGPNRAGLPFMKGKGPRLFGLTQPFADAVKALTKEDIVP
                     ANADKRLHFFAPVVVCTFALMTFAVIPFGREVIPVELDAAVLYFFAAGSATELAIFTA
                     GWSSRNKYSLLAAMRALAQLISYELPLLLTVVPVIMLSGTLSTQNIVLAQSGWAGGIV
                     PNWHIFTPWGALGFLIFLIAALAETNRSPFDLPEAESELIAGHLTEYSGFKYALFFMA
                     EYFGMTALCALGVTLFLGGWQSPFAVLQFIPSYVWFALKLITLVLVLIWIRGTLLRLR
                     IDQLTRLSWKFLVPLALINLFNAAFWWKTADFPPALQIVRWLVSIAWVVLPFISLGRR
                     LSAGLAPRTYRYA"
     gene            1335..1880
                     /locus_tag="VEA63_13085"
     CDS             1335..1880
                     /locus_tag="VEA63_13085"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009509696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HYD84990.1"
                     /translation="MIPLTIIAALMLASAALAMTRRNLIHAALLLIVTWASIACFYLW
                     AGAEFVAFGQILIYVGAVSMVVLFAVLLTRPTLVDPVDSAASRRRVAIALLAGAALLA
                     VLAYAILNTPFALQENRPPHIEVRQLGFQLMGPHAASLLVVGVLLTVALIGAAVIAAT
                     EDKDGSPSRPPRPPTSRNADT"
     gene            1877..2197
                     /gene="nuoK"
                     /locus_tag="VEA63_13090"
     CDS             1877..2197
                     /gene="nuoK"
                     /locus_tag="VEA63_13090"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009509695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HYD84991.1"
                     /translation="MSVLTPLHLCLLLSSALFAIGLVVALTRSNSILVLLGIELMLNS
                     ANLNFIAFWRFGPTPAPATGVVFVLFAIAVAAAEAAVGLALVIAIYRHQKTIRLQEAN
                     RLKG"
     gene            2348..4411
                     /gene="nuoL"
                     /locus_tag="VEA63_13095"
     CDS             2348..4411
                     /gene="nuoL"
                     /locus_tag="VEA63_13095"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007363718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HYD84992.1"
                     /translation="MTLSAQHLFLIPALPLLAAAIGALLPRHQPRLAATTAIAGISAS
                     FACSVIALIVVLRDPTIRHTFNFAWFDLGAEPLRLGLLLDPLTAFMCVMVTFVSTLIF
                     IFSLGYMKHDANVTRFFCFLSLFAAAMLGIVVSNSLLLLFISWELVGLASYLLIGFWF
                     HKPSAAAAAKKAFITTRIGDLGFLLGLVWLYHATGSLLLFDHGAGAMEPAALNALSTQ
                     QLACGLGVSAGIGLLLFCGAIGKSGQFPLHVWLPDAMEGPTPVSALIHAATMVAAGVF
                     LMARIYPLMTADQLLADAPFHAMTVVAFIGAITALLGACLAVAQNDLKRVLAFSTVSQ
                     LGYMMLAIGVGSWVAAIFHLLTHAFFKALLFLGAGSVIHAAHHEQDIRRLGGLAPKMK
                     LTFATFAIGTMALAGVPFLFSGFWSKEAIIHAAHAWPVSHLPFVIALVAVALTAFYMT
                     RLVAEVFFGQSRSPAAEHAHENSATMTVPLVLLAAAAILLGFLGTPAWPWLQTQLLAP
                     LTHQATVLIDPHLPGPWHALIETPALLFLSIVLVGAGISLGWWIYGRKTRATADALDP
                     LEAAAPRAFTALANRLGFDELYAATVGRLVSALAILADLFDRYIWDGAVRATAAFARV
                     FGLMNQQADDAVLNAGFDAASEKLRASGRAYSRAQTGHAHGYLLTLALAFVALAIALM
                     IGGAR"
     gene            4512..>5981
                     /locus_tag="VEA63_13100"
     CDS             4512..>5981
                     /locus_tag="VEA63_13100"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007363719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HYD84993.1"
                     /translation="MTALPLLSLVVFTPWLGAFVLAALPRLSANAARHTALAFSFSTL
                     VFALVLVAAFDPYAGLQFTEHHAWIPSLHVAYHLGLDGMSLLLVLLTGIVSPMALLAS
                     YRETRDVRLFGSLFLFLQGAALGVFLALDFFHWFLFWELSLVPAFFLIKLWGGGSTPR
                     GRAAASRAAYQFFIYTIGGSVFLLAGFAALSTAAGTFDFIQLAALRADGTLAVQLGGT
                     WNSVIFAGIFLGLAVKVPLFPFHTWLPSAYAEAPTGASMFLTGVMSKMGLYGFLRILW
                     PLFPDELHTFATPLLWLALGGVVLGAFAALRQTDLKRMIAYSSVNHLSYCLLALFAVA
                     AAASPANEAATVSALSGTLLQMFNHGLSATALFFCIGVLETRNAGFRGLNDFGGVRTA
                     MPIFAGLCGIALFSSLGLPGLNGFVGEFLIFRGVFGLAPWAAALALLRLFATTLFLLT
                     FWQRVFHGPQRGAVATFTDLSKQEIAVLIPATGLMFGLGI"
BASE COUNT          836 a         2385 c         1489 g         1271 t
ORIGIN      
        1 tcgcgccgtc caacttcctc gaacacgccc cgctcgccgc gcgcgactgg ctcgtatcca
       61 ttttcccagc accgctgcat tggctcgtcc accacctgat tacgatcatc gcgattctcg
      121 ccgtcttcgg cctgtttttc gccctgctca ccgtcgtcga acgcaaactt ctcgcccgca
      181 tccagaatcg cctgggcccc aaccgcgccg gcttaccctt tatgaagggc aaaggcccgc
      241 gtctcttcgg cctcacgcaa cccttcgccg atgcggtgaa agcgctcacc aaggaagaca
      301 tcgttcccgc caacgccgac aaacgccttc acttcttcgc gcccgtcgtc gtgtgcacgt
      361 tcgcgctgat gacgttcgcc gtcatcccat tcggccgcga agtcatcccg gtcgagctcg
      421 acgccgccgt gctctatttt ttcgccgccg gctccgccac cgaactcgcg atcttcacgg
      481 ccggctggtc cagccgaaac aaatactcgc tgctcgccgc catgcgcgcg ctcgcccaac
      541 tcatcagcta cgaactcccg ctgctgctca ccgtcgtgcc cgtcatcatg ctctccggca
      601 cgctctcgac ccagaacatc gtcctggccc aaagcggctg ggccggaggc atcgtgccca
      661 actggcatat tttcacccct tggggcgcgc tcggttttct catctttctc atcgccgcgc
      721 tcgcggaaac caaccgctcg ccgttcgact tgcccgaagc cgagagcgaa ctcatcgccg
      781 gtcatctcac cgagtattcg ggtttcaaat acgccctctt cttcatggcc gaatacttcg
      841 gcatgaccgc cctttgcgcc ctcggcgtca cgctcttcct cggcggctgg cagtcgccct
      901 tcgccgttct gcaattcatc ccttcctacg tctggttcgc gttaaaactg atcaccctcg
      961 tcctcgttct gatctggatc cgcggcacat tgctccgcct gcgcatcgac cagctcacgc
     1021 ggctctcctg gaaattcctc gttccgctcg cgctcatcaa cctgttcaac gccgcgttct
     1081 ggtggaaaac cgccgacttc ccgcccgcac tccaaatcgt ccgctggctc gtctcgatcg
     1141 cctgggtggt gctgcccttc atctccctcg gccgccggct gagcgccggc ctcgcaccgc
     1201 gaacctaccg ctacgcgtga tgaaattttc gaatttcgat tttcgaattt cgatttcccg
     1261 tctgacacga cgcggatggc gaatcgcgcc ggtttggaga aatcgaaaat cgaaaatccc
     1321 aaatcgaaaa tgaaatgatc cccctcacca tcatcgccgc cctgatgctc gccagcgccg
     1381 cgctcgcgat gacgcgccgc aatctcattc acgccgcgct cctcctcatc gtcacctggg
     1441 ccagcatcgc ctgcttttat ctctgggccg gtgccgagtt cgtcgccttc ggtcaaatcc
     1501 tcatctacgt cggcgccgtt tcaatggtcg tgctcttcgc cgtcctcctc acgcgcccca
     1561 ccctcgtcga tcccgttgac tccgccgctt cgcgccgccg cgtcgccatc gcgttgctcg
     1621 ccggtgccgc cctgctcgcg gtgctggctt acgccattct caacaccccg ttcgccctcc
     1681 aggaaaaccg cccgccgcac atcgaagtcc gccaactcgg ctttcaactg atgggtccgc
     1741 acgcggcctc cctgctcgtc gtcggcgtgc tgctcaccgt cgcgctgatc ggcgccgccg
     1801 tcatcgccgc caccgaggac aaggacggct ctccgagccg tccgcctcgc cctcccactt
     1861 ctaggaacgc cgacacatga gcgtgctcac cccgcttcat ctctgcctcc tgctctcgag
     1921 cgcccttttc gcgatcggtc tcgtcgtcgc gctcacgcgc agcaacagca tcctcgtcct
     1981 cctcggcatc gaactgatgc tcaactccgc gaacctgaac ttcatcgcgt tctggcgctt
     2041 cggccccacg cccgcgcccg caaccggcgt ggtcttcgtc ctcttcgcga tcgccgtcgc
     2101 cgccgccgag gccgccgtcg gcctcgccct cgtgatcgcg atttaccgcc atcagaaaac
     2161 catccgtctg caggaggcaa accgcctcaa gggatgagac gaggccaagc tccaagcgct
     2221 ccaagcacca agctccagtg aattttcaat ctttcaacgc ccaacccgac cgagcctccg
     2281 caggtttgag gtttggaact tcaatggagc ttggagctcg gtccttggcg ctttcgccgc
     2341 cgcgtcgatg accctctccg ctcagcatct ttttctcatc cccgcgctcc ctctgctcgc
     2401 ggcggcgatc ggcgctttgc tgccgcgcca ccaaccccgc ctcgccgcca ccaccgcgat
     2461 cgccggcatc tccgcttcgt tcgcctgctc cgtcatcgcg ctgatcgtcg tccttcgcga
     2521 tccgacgatt cgccacacgt tcaacttcgc ctggttcgac ctcggcgccg agccgcttcg
     2581 cctcggcctg ctcctcgatc cgctcaccgc gttcatgtgc gtgatggtca cgttcgtcag
     2641 cacactgatc ttcatcttca gccttggcta catgaagcac gacgcgaacg tcacgcgctt
     2701 cttctgcttc ctcagtttat tcgccgccgc gatgctcggc atcgtcgttt ccaacagcct
     2761 gctgctgctc ttcatctcct gggaactcgt cggcctcgcc tcgtatctcc tcatcggctt
     2821 ctggtttcac aaaccttccg ccgccgcagc ggcgaaaaaa gccttcatca ccacgcgtat
     2881 cggcgacctc ggttttctcc tcggactcgt ctggctctat cacgcgaccg gctcgctgct
     2941 ccttttcgat cacggcgccg gcgcgatgga acccgccgcg ctcaacgcac tttccacgca
     3001 acaactcgcc tgcggtctcg gcgtctccgc cggcatcggc ctgctgctct tctgcggcgc
     3061 catcggcaag tccggccagt tcccgcttca cgtctggctg cccgacgcga tggaaggccc
     3121 cacgcccgtc tccgcgctga tccacgccgc caccatggtc gccgccggcg tgtttctgat
     3181 ggcgcggatt tatccgctca tgaccgcgga ccagcttctc gccgacgcac cgtttcacgc
     3241 gatgaccgtc gtcgctttca tcggcgccat caccgcgctc ctcggcgcct gcctcgccgt
     3301 cgcgcaaaac gacctcaagc gcgtcctcgc cttctccacc gtttcccaac tcggctacat
     3361 gatgctcgcg atcggcgtcg gctcctgggt cgctgcgatc ttccacctgc tcacgcacgc
     3421 gttcttcaaa gccctgctct tcctcggcgc cggctccgtc atccacgccg cgcatcacga
     3481 gcaggacatc cgtcgactcg gcggactcgc gccgaaaatg aaactcacct ttgccacctt
     3541 cgccatcggc acgatggcgc tcgccggcgt gccgtttctc ttctccggct tctggagcaa
     3601 ggaagccatt atccacgccg cgcacgcctg gcccgtgtcg cacctgcctt tcgtgatcgc
     3661 cctcgtcgcc gtcgcgctca ccgcgttcta catgacgcga ctcgtcgccg aagttttctt
     3721 cggccaatcc cgctcgcccg ccgccgagca cgcgcacgaa aactccgcga caatgacggt
     3781 gcctctcgtc ctcctcgccg cggccgcgat cctcctcggc ttcctcggca cgcccgcctg
     3841 gccgtggttg caaacccaac tcctcgcgcc gctcacgcac caagccaccg ttctcatcga
     3901 tcctcacctc cccggcccct ggcacgcact gatcgaaacg cccgcgctcc tgtttctctc
     3961 catcgtcctg gttggcgccg gcatcagcct cggctggtgg atctacggcc gaaaaacccg
     4021 cgccaccgcc gacgcactcg acccgctcga agccgccgca ccgcgcgcgt tcaccgccct
     4081 cgcaaaccgc ctgggtttcg acgaactcta cgccgccacc gtcggccgcc tcgtatccgc
     4141 actcgcaata ctcgccgatc tcttcgaccg ctacatatgg gacggcgccg tgcgcgccac
     4201 cgccgccttc gcccgcgtgt tcggtctgat gaaccagcaa gccgacgacg ccgtgctcaa
     4261 cgccggcttc gacgccgcca gcgaaaaact tcgcgccagc ggccgcgctt actcgcgcgc
     4321 ccaaaccggc cacgcccacg gctacctgct cacgctcgcc ctcgccttcg tcgcgctcgc
     4381 gatcgccctc atgatcggag gcgcccgatg aagacaccgg tagggacgcc tctccgaggc
     4441 ctccgttcga atagcgaaaa tctcacacgg acggctcgga gagccgtccc taccaacccg
     4501 gaggcaatcg catgaccgcc ctcccgctcc tctccctcgt cgtcttcacg ccttggcttg
     4561 gcgccttcgt gctcgccgcg ctgccccgcc tcagcgccaa cgccgctcgt cacaccgccc
     4621 tcgccttcag cttttccacg ctcgtcttcg cgctcgttct cgtcgccgct ttcgaccctt
     4681 acgccggact ccaattcacc gagcatcacg cgtggattcc gtcccttcac gtcgcgtatc
     4741 atctcggcct ggacggcatg agcctcctgc tcgtcctcct caccggtatc gtttcgccga
     4801 tggcgctcct cgcctcgtat cgcgaaaccc gcgacgtccg acttttcggc agcctgttcc
     4861 ttttcctcca gggcgccgcg ctcggcgtat tcctcgcact cgattttttt cactggttcc
     4921 tcttctggga actcagcctc gtgcccgcgt ttttcctgat caaactctgg ggcggcggtt
     4981 cgactccacg cggccgcgcc gccgcctcgc gcgccgcgta tcagtttttc atctacacga
     5041 tcggcgggag cgtcttcctg ctcgccggct tcgccgcgct ctccaccgcc gccggcacgt
     5101 tcgacttcat ccaactcgcc gccctccgcg ccgacggcac gctcgccgtg caactcggcg
     5161 gcacatggaa cagcgtcatc ttcgctggaa tttttctcgg cctcgccgtc aaagtcccgc
     5221 tcttcccgtt tcacacctgg ctgccctcgg cctacgccga agcgccgacc ggcgcatcga
     5281 tgtttctcac cggcgtgatg tcgaaaatgg gcctctatgg cttcctccgt atcctctggc
     5341 cgcttttccc ggacgagctg cacacgttcg ctacgccact gctgtggctc gcactcggcg
     5401 gggtcgtgct cggcgcgttc gccgcgctcc gccagaccga cctgaaacgc atgatcgcgt
     5461 attcgtccgt caaccacctg agctactgcc tcctcgcgct cttcgccgtc gccgccgccg
     5521 cttcgcccgc caatgaagcg gccaccgtct ccgcgctcag cggcacgctg ctgcagatgt
     5581 tcaaccatgg cctctccgcc accgcgctgt tcttctgtat cggcgttctg gagacgcgca
     5641 acgccggctt ccgcggcctg aacgacttcg gcggcgtgcg caccgccatg ccaattttcg
     5701 ccgggctctg cggcatcgcc ctgttctcct cgctcggcct gcccggcctc aacggcttcg
     5761 tcggcgagtt tctcattttc cgcggcgtgt tcggtctcgc gccgtgggcc gccgccctcg
     5821 cgctgctccg cctgttcgcc accacactgt ttctgctgac cttctggcag cgtgtgtttc
     5881 acggtccgca gcgcggcgcc gtcgcgacct tcaccgacct ttcgaagcag gagatcgccg
     5941 tcctgattcc cgccaccggc ctgatgttcg gcctcggcat c
//
LOCUS       DASPLT010001281         1390 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_200212, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001281 DASPLT010000000
VERSION     DASPLT010001281.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1390)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1390)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1390
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_200212"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(242..>1390)
                     /locus_tag="VEA63_13105"
     CDS             complement(242..>1390)
                     /locus_tag="VEA63_13105"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HYD84994.1"
                     /translation="QPTVEFKKIVLTEEYLADGISAGDFNRDGKPDVVAGPYWYEGPD
                     FKTRHAFYPAEPQDVAATPSNSLFSFVADFNGDGWDDILVLGRVMHHQAFWYENPRDA
                     GDGWSKHFAIHRVYGEAPMFVDMEGDGRAEVISFWEDQWGWWMPNPAEPTKPWAFHPV
                     TEKGKYREYYHGSGVGDINGDGRMDLVLNEGWWEQPAEKGALWKHRAVKFSHGRGGAQ
                     ILVYDIDGDGDNDVVSAIDAHGWGLSWFEQVKRNKDQIEFVEHRMMGDRSEEAKFGVA
                     FSQPHALCLGDPDGDGLMDVITGKRRWAHGPKGDVEPMGTPVNYWFRLQRDGRGGASF
                     TPVLIDDASALGTQVEAIDVTGDGLPDVLTASKLGAFIFVPEKVSSSR"
BASE COUNT          268 a          485 c          375 g          262 t
ORIGIN      
        1 aaatcccacc gcaaacgaac tcggcgccgc gtccggcatc agcgttcgtt cgatcgacaa
       61 atcgtccgtg ttgggccgag ccgccgcccc gatcgacacc gcgaacgcgg cggcgagcag
      121 gtgtatccgc tttttcaatg cgagtggtat catcgtgaaa ttctgagttg gaatgcgctt
      181 cgcgcttcgc cgaagcagtt ggaatcagcc cgccgcatcg cccgggtgca tgggccgcgg
      241 ctcaccggga agacgagact ttctccggca cgaaaatgaa tgcgcccaat ttcgatgctg
      301 tcaggacatc cggcaatccg tcgccagtca catcgatcgc ctccacttgc gtgcccagcg
      361 ccgatgcgtc gtcgatcaac accggcgtga acgaggcgcc gccgcggccg tcgcgctgca
      421 ggcgaaacca ataattgacc ggcgtgccca taggctcgac gtcacctttc gggccgtgcg
      481 cccagcgccg cttgcccgtg atcacgtcca tcaagccgtc gccgtcggga tcgccgaggc
      541 aaagcgcgtg cggctgcgaa aacgcgacgc cgaacttcgc ctcctccgac cgatcgccca
      601 tcatgcgatg ctccacaaac tcgatctggt ctttattacg cttcacctgc tcgaaccacg
      661 aaagccccca gccgtgtgcg tcgatcgcgg agacgacgtc gttgtcgcca tcgccatcga
      721 tatcataaac gaggatctgc gcgccgccac gcccgtggct gaacttcacc gcgcgatgct
      781 tccacagcgc gcccttctcc gccggctgct cccaccagcc ttcgttcaac acgaggtcca
      841 tgcggccatc gccattgatg tcgcccacgc ccgagccgtg ataatattcg cggtatttgc
      901 ctttctcggt gacgggatga aacgcccaag gcttcgtcgg ttccgccgga ttcggcatcc
      961 accagcccca ttgatcctcc caaaaactga tcacctccgc tcggccatca ccctccatgt
     1021 ccacaaacat cggcgcctcg ccgtaaacgc ggtgaatcgc gaaatgcttg ctccagccgt
     1081 cgcccgcatc ccggggattc tcataccaga acgcctggtg atgcatcacg cggcccagaa
     1141 cgagaatatc gtcccagccg tcgccgttga aatctgccac gaaggaaaac agactgttgg
     1201 agggagtcgc cgcgacgtcc tgcggctccg ccggataaaa cgcgtgccgc gtcttgaagt
     1261 ccggcccctc ataccaatag ggccccgcga ccacgtccgg tttgccgtcg cggttgaaat
     1321 cgcccgcgct gattccatcg gcgagatatt cctcggtcag gacgatcttt ttaaactcaa
     1381 cggtcggttg
//
LOCUS       DASPLT010001282         1032 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_15836, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001282 DASPLT010000000
VERSION     DASPLT010001282.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1032)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1032)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1032
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_15836"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            133..1032
                     /locus_tag="VEA63_13110"
                     /pseudo
     CDS             133..1032
                     /locus_tag="VEA63_13110"
                     /inference="COORDINATES: protein motif:HMM:NF023295.4"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction enzyme endonuclease
                     domain-containing protein"
BASE COUNT          200 a          325 c          311 g          196 t
ORIGIN      
        1 atggcgaacc gcttcaagga ccccgcggat ccgttccgca tcgtcatcgt gcgcgacatg
       61 tggctcaccg gcttcgacgc gccgtgcctg catacgctct atgtcgataa gccaatgcgc
      121 ggccacgggc ttatgcaagc gatcgcccgc gtgaaccgcg tcttcggcga caagcccggt
      181 ggcttggtgg tggactatct cggcatcggc gccgaactga aagccgcgct tgctgcttac
      241 agcgctcgcg acaaggaaag tgtcggcgtc gatcaggacg cagccgtgcg cgagttggct
      301 tcaacctacg aagcgatcgt tgatttcttc aacggcttac cctggcagga tttcttctcg
      361 gccaattcag ccggcaagct caacgtgctg cgcaacgctg tcgattacgc actctcactc
      421 gaaaatggac gcacccgttt ccttgacctc gcagcgcgcc tcgccaccgc cttcgcgctg
      481 gcggcgggaa cggccaaggc cgcggagtta cgcgatcacg tagcgctctt catcgccgtg
      541 cgcgccaatc tgatcaagca caccggcggc ggcaaagacc ctgaagacat cgatcacgaa
      601 ctcaatcagc tcgtttctcg cgccgtgctt gcggatggta ttttgacgtg ttcaaggcgg
      661 ccgggctcga acgcgccgac atctcgctcc tatccgacga attcctcgcg gacgttcagg
      721 gcatcgaaca aaagaacctg gcgatcgagg cgcttcggaa gctgctcgcc ggcgccgtaa
      781 aggcgcgcca ggcgcacaac gtcgttgaag cacggcgttt ttcggagcgt ctggaagacg
      841 cgatggcgcg ctaccacaat cgcgccgttg atagcctgca agtgatccag gagctcatcg
      901 agctcgccaa ggagatgcgc gaagcgggca agcgcggcgt ggacctcgga ctgagtgagg
      961 acgaactcgc cttctatgat gcgctggctg acaatcggag cgccgttgat ctgttgggca
     1021 acgacacttt gc
//
LOCUS       DASPLT010001283         1292 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_219673, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001283 DASPLT010000000
VERSION     DASPLT010001283.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1292)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1292)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1292
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_219673"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..270
                     /locus_tag="VEA63_13115"
     CDS             <1..270
                     /locus_tag="VEA63_13115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD84995.1"
                     /translation="QNKCGLVNTANPCRCTKKAGAFMRNGWLDPKRRQFTAERLASVR
                     EAAAGRLEELQAAQRAYADVYRDTPLTDAPAILERIRATFVSSES"
     gene            761..>1292
                     /locus_tag="VEA63_13120"
     CDS             761..>1292
                     /locus_tag="VEA63_13120"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HYD84996.1"
                     /translation="MNSKNDADLVQLARAGNRDAYGELIRRYQRSICGLACLLIGDRF
                     EAEDLTQEAFLRAWLNLDLLSDPSKFAPWLRRIVFGVSIDWLRVFRPDLYRSRDVEAE
                     LELSLQPIKTEAAFGPLEAIELRQRIWDAVSRLPPRYRLPLTLFHIDGLSHSKVAEAL
                     GISVSTVRSLVTRARQK"
BASE COUNT          239 a          372 c          421 g          260 t
ORIGIN      
        1 cagaacaaat gcggcctggt gaacacggcg aatccgtgtc gttgcacgaa gaaggccggc
       61 gccttcatgc gcaacggctg gctcgatccg aagcggcgtc agttcacggc cgaacggctg
      121 gccagcgtgc gagaggccgc cgccggccgc ctcgaagaac tgcaagccgc gcagcgcgcc
      181 tacgccgacg tgtatcgtga cacgccgctg accgacgcac ctgcgatcct cgagcgtatt
      241 cgcgccactt tcgtgtcatc tgaatcctga cggatgccga gcgaggagag cgggcactca
      301 cagcaaacta agctgcgaat cgtccgcggg cgtgacggtg atttttttct tcggcccggc
      361 ctgtggcgtg gcggcggggg agcgcgacgc tggtgtcgtc gctctccagt ttgctgagga
      421 tggtttggcg cgatcgatca cgctcaacgg gaggccggcg aggcgggcga cgtgatgccg
      481 tagctgcggt cggcggcgcc ggggatcacg cggcggacga agacgatttc gtcgttccac
      541 tcttttaccg cgacggagaa tgttcgcggt ttggttggcg ctaaccttga acgttgggcg
      601 agcgaggggg tttcgacggc tcgaaggaag ccgacataaa acagctagtg tctcaatgca
      661 gaggtctcca tccgatcgcg gacgggaccg tctgggaaac gctgtccgtg aaatttctgc
      721 agcgttttct cactgagagt cgactactct caggatgcag atgaactcga agaatgatgc
      781 cgaccttgtc caactcgcga gggcgggaaa tcgggacgcc tatggagaac tgatccggcg
      841 ctaccaacgc tcgatctgcg gattggcgtg cctcctgatt ggcgatcgat tcgaggccga
      901 ggacctgacc caggaagcgt ttctccgcgc gtggctgaat ctcgatcttc tcagcgatcc
      961 atcaaagttt gcgccgtggc ttcggcggat cgttttcggg gtttccatcg attggctccg
     1021 ggtgtttcgt ccggacctct acaggtcgag ggatgtcgaa gccgagttgg agctttctct
     1081 tcaaccgatc aaaaccgagg ccgcttttgg accgctcgaa gcgatcgaac ttcggcaacg
     1141 gatttgggac gccgtctccc gcctccctcc gcgataccgg ttgccgttga cgctgtttca
     1201 tatcgacggt ctgagccaca gcaaggtagc cgaagctctc ggcatatcgg tgagcacagt
     1261 gaggtcgctg gtgacgcgcg cgcgacagaa gc
//
LOCUS       DASPLT010001284         1649 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_75805, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001284 DASPLT010000000
VERSION     DASPLT010001284.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1649)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1649)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1649
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_75805"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1494
                     /locus_tag="VEA63_13125"
     CDS             <1..1494
                     /locus_tag="VEA63_13125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD84997.1"
                     /translation="GYLAVLSLAAAFFLAVNSAGAAVVERPLGGFSNHGDVGAVSRSV
                     AASYDAATKTYAVGASGRDIWGAEDAFGFVWKQVEGDVAIAADVALLGSSAEPHRKAG
                     LMFRQSLAASSTYADVVVHGDGHVALQFRSEPGGPARTIEGAASAPKRVRLEKRGAYV
                     TLSFSDAEGRWVASGCSTRLEFDGPFYVGLAVTAHDDAAFETATFKAVELSTPPPRSI
                     TRGSALEIVELASLDRRVIHSTSGKMEAPHFSADGKALYYNHEGRIKRVPLDHNEAPT
                     IIDTGFATRCINDHGLSPDGTQLVISDLTETGKSRMYLLPVGGGTPKRVEAAEPALWH
                     GWSPDGKTLTYCAQRDGNYDVYTIPVEGGAETRLTTAPGNDNGPDYSADGKWIYYHSV
                     RENRVQVWRIHVDGTNPEQVTNDDFDNWFPHPSPDGKWILVLSTKVALETGHPPDGDY
                     VLRLLPVGGGEAREIVTLYGGNGSLNVPCWSRDSTKIAYASYEPPAN"
BASE COUNT          312 a          468 c          581 g          288 t
ORIGIN      
        1 ggttatctcg ccgttctttc gcttgccgct gctttcttcc tcgccgttaa cagcgcgggc
       61 gccgcggtgg tcgagcggcc gctcggcggg ttttcgaatc atggagatgt cggcgcggtg
      121 agccggtccg tcgctgcgag ctacgatgcg gcgacgaaga cgtatgccgt tggcgcgagt
      181 ggcagggata tttggggagc agaagacgcg ttcggtttcg tgtggaaaca agtcgagggc
      241 gacgtcgcga ttgcggcaga cgtcgcgttg ctcggatcga gcgcggagcc gcatcgcaag
      301 gcgggattga tgtttcggca gtcgctcgcc gcgagctcca cctacgcgga tgtcgttgtg
      361 cacggcgacg ggcatgtggc gttgcagttt cgcagcgaac cgggcgggcc ggcgcgcacg
      421 atcgaggggg cggcgtccgc accgaagcgc gtgcggctgg agaaacgcgg cgcttatgtg
      481 acgttgtcct tcagcgatgc cgaagggcgg tgggtggcgt cgggatgttc gacgcggctc
      541 gaattcgacg gaccgtttta cgtcgggctt gctgtgaccg cgcacgacga cgctgcgttc
      601 gagacggcga cgttcaaggc ggttgagctg agcacgccgc cgccgcgttc gatcacgcgc
      661 gggtcggcgt tggaaatcgt ggaactcgcg tcgctggatc ggcgcgtgat tcacagcacg
      721 tcggggaaga tggaagcgcc gcatttttcc gccgacggaa aagcgctgta ttacaatcac
      781 gaaggccgga tcaagcgcgt gccactcgac cacaacgaag caccgacgat tatcgacacc
      841 ggtttcgcga cgcgttgcat caacgatcac gggctctcgc ccgacggcac gcaactcgtc
      901 atcagcgacc tcacggaaac ggggaagtcg cgcatgtatt tactgccggt gggtggcggc
      961 acgccgaaac gcgtcgaggc ggcggagccg gcgttgtggc acggctggtc gccggatggg
     1021 aagacgctga cctactgcgc gcagcgcgat ggcaactacg acgtttacac gatcccggtc
     1081 gagggcggcg cggagacgcg gctcacgacg gcgccgggca acgacaatgg gccggactac
     1141 tcggcagatg gaaaatggat ctactatcat tcggttcgcg agaatcgtgt ccaagtctgg
     1201 cggatacatg tcgacggcac gaatccggag caggtgacga acgacgattt cgacaactgg
     1261 tttccgcatc cgtcgccgga tggaaaatgg attctcgtgc tttcgaccaa ggtcgcgctt
     1321 gaaacgggac acccgcccga cggcgattat gtgctccgtt tgcttcccgt cggcggtggc
     1381 gaggcgcgag agatcgtcac gctttacggc ggcaacggtt cgctcaatgt tccgtgctgg
     1441 tcgcgcgaca gcacgaagat cgcgtatgcg agttacgagc cgccggcgaa ctgaggtgcg
     1501 tgcgaccgag cgaacaaacg cagcatcgac gcacgaagcc gacatgattg gccgcaagaa
     1561 cgcgcaagaa gtcgcaagag gcaagacgga ctcatgccgc ggaaatggtg cggccgtcga
     1621 ggaagctgca caccgaaagg tggagcccg
//
LOCUS       DASPLT010001285         2255 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_75807, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001285 DASPLT010000000
VERSION     DASPLT010001285.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 2255)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2255)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2255
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_75807"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..694)
                     /locus_tag="VEA63_13130"
     CDS             complement(<1..694)
                     /locus_tag="VEA63_13130"
                     /inference="COORDINATES: protein motif:HMM:NF016176.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HYD84998.1"
                     /translation="MKAVSKLILVTVRGALALGLVLPARAATETYKIQASHSNVGFNV
                     KRFFTKIPGSFATVDGTIVVDRDALENSSVDATIAIGSVNTNDAKRDGHLKNDDFFSA
                     DKFPSASFKSTSWKKTGADTYDVAGNLTIKDVTKPVILKTKLLGFGKAARGLEVAAFE
                     ATTTLDRREFGITYGAPAIASEVDVVINIESQKQTTGAAANPPPPATKPTAAVNGNFI
                     PEGVWRGVFDISE"
     gene            complement(813..971)
                     /locus_tag="VEA63_13135"
     CDS             complement(813..971)
                     /locus_tag="VEA63_13135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD84999.1"
                     /translation="MKTRIATLSLLAFVAALTALASLFGHSRRALDADSPFGPTSLLR
                     PSSPIARG"
     gene            complement(984..>2255)
                     /locus_tag="VEA63_13140"
     CDS             complement(984..>2255)
                     /locus_tag="VEA63_13140"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HYD85000.1"
                     /translation="TASLAAPHTLPAGVTSLASVDDPANNKPNAREALTPGDRRGLPL
                     SVTWKFANHTLDAGAWWEEENSDSIRRLHNVQGGVITNPFDWSTFITAYFNQQTKLST
                     QQYFLKDTIKLLDDQLAISAGIKALSVTTDFQGYPDSTYFDRGQWIHRKPTYSDWFLP
                     QLGATYALTRSDELFFNYAENFAAPSTSVIGGTVFDKSKLRPERAHNFDLGVRTSRGR
                     LSASLAGYVIEYKNRIGDVTAYDPLGFGTANTATNYVNVGRVRGYGTELAVAYSPTRD
                     FRLNLAAAWQSLTYGDNYTEATSTGGTIVRAIKGNTIPNTPRWNANLDATHFFGPFFI
                     AANAHYQGDVFLTTNNAQRIPGYTLLGIGVGYDGISRRGPLKNIRIALNIENALDRYW
                     FYTNGASTAFANGSFSVGTPRAYFLTVSGKF"
BASE COUNT          362 a          529 c          924 g          440 t
ORIGIN      
        1 cctcggagat gtcgaagacg ccgcgccaca cgccttcggg gatgaagttg ccgttcacgg
       61 cggcggtggg tttcgtggcg ggtggcggcg ggttggcggc ggcgccggtg gtttgtttct
      121 gcgattcgat gttgatcacg acgtcgacct cgctggcgat tgcgggcgcg ccgtaggtga
      181 tgccgaactc gcggcggtcg agcgtggttg tcgcttcgaa cgccgcgacc tcgagaccgc
      241 gcgcggcttt gccgaagccg agaagcttcg ttttcaggat gacgggcttg gtgacgtcct
      301 tgatcgtgag attgccggcg acgtcgtagg tgtcggcgcc ggtttttttc cacgacgtgc
      361 tcttgaagga ggcggagggg aatttatcgg cggagaagaa gtcgtcgttc ttcagatggc
      421 cgtcgcgttt cgcgtcgttg gtgttgactg aaccgatcgc gatcgtggcg tcgacgctgc
      481 tgttttcgag cgcgtcgcgg tcgacgacga tggtgccgtc gaccgtggcg aagctgccgg
      541 gaattttggt gaagaagcgt ttgacgttga agccgacgtt ggagtgggag gcttggattt
      601 tataggtttc cgtcgcggcg cgcgcgggga gaacgaggcc cagcgcgagg gcgccgcgca
      661 cggtgacgag gatgagtttc gatacggctt tcatgaacga ggttcgatgg cgtgagcggc
      721 gggcgtccgg cgttcgggtg gcggcagcaa ggaggaacgg gagagtcgcg tctggtgacg
      781 tggagcgaaa aggaacgtgc gcaggatcga gcttaaccgc gggcgatggg ggacgatgga
      841 cggagaaggc tcgtgggacc gaagggggaa tcggcgtcga gcgcgcggcg ggagtggccg
      901 aagagcgagg cgagggcggt gagcgcggcg acgaacgcaa ggagcgagag cgtggcgatg
      961 cgggttttca tggaggagcg gtgtcagaac tttccggata cggtgagaaa gtaggcgcgc
     1021 ggggtgccga cgctgaagct gccgttggcg aacgcggtgc tggcgccgtt ggtgtagaac
     1081 cagtagcggt cgagggcgtt ctcgatgttg agcgcgatgc ggatgttctt cagtgggcct
     1141 cggcgcgaaa tgccgtcgta gccgacaccg atgccgagga gcgtgtagcc cgggatgcgc
     1201 tgggcgttgt tggtcgtgag aaaaacgtcg ccctggtagt gggcgttggc ggcgatgaag
     1261 aaggggccga agaagtgggt ggcgtcgagg ttggcgttcc agcgcggcgt attcggaatt
     1321 gtgttaccct tgatcgcgcg gacgatcgtg ccgccggtcg acgtggcttc ggtgtagttg
     1381 tcgccgtagg tgagcgactg ccaggcggcg gcgaggttga ggcggaagtc gcgcgtcggc
     1441 gaatacgcga cggcgagttc ggtgccgtag ccgcggacac ggccgacgtt gacgtagttg
     1501 gtggcggtgt tggcggtgcc gaagccgagc gggtcgtagg cggtgacgtc gccgatgcgg
     1561 tttttgtatt cgatcacgta gccggcgagc gacgcgctca gccggccgcg cgaggtgcgc
     1621 acgccgagat cgaagttgtg cgcgcgctcg gggcggagtt tggatttatc gaagacggtg
     1681 ccgccgatga cggaggtcga gggcgcggcg aagttttccg cgtagttgaa aaacagctcg
     1741 tcggagcggg tgagggcgta ggtggcgccg agctgcggaa ggaaccagtc ggagtaggtc
     1801 ggcttgcggt ggatccactg gccgcggtcg aagtaggtgc tatctggata cccttgaaaa
     1861 tcggtggtga cggagagcgc cttgatgccg gcggaaatgg cgagctggtc gtcgagaagc
     1921 ttgatcgtgt ctttgaggaa atactgctgc gtcgagagct tcgtctgctg gttgaaatag
     1981 gcggtgatga aggtcgacca gtcgaacggg ttggtgatga ctccgccctg gacgttgtga
     2041 aggcggcgga tggaatcgga gttttcctct tcccaccagg cgccggcgtc gagggtgtgg
     2101 ttggcgaatt tccaagtgac gctgagcggg aggccgcggc ggtcgccggg ggtgagcgct
     2161 tcgcgggcgt tgggtttgtt gttggcggga tcgtcgacgc tggcgagcga cgtcacaccg
     2221 gcgggaagcg tgtggggcgc ggcgagcgag gcggt
//
LOCUS       DASPLT010001286         1116 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_158255, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001286 DASPLT010000000
VERSION     DASPLT010001286.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1116)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1116)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1116
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_158255"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            306..1067
                     /locus_tag="VEA63_13145"
     CDS             306..1067
                     /locus_tag="VEA63_13145"
                     /inference="COORDINATES: protein motif:HMM:NF024743.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HYD85001.1"
                     /translation="MKTHLLSLLLFGFVAAAPASLFSATESFSRSAPFDPTGKVSLHN
                     VNGDVTVETWDRNEIAIEGEKNARTEEELKRIDLAIELLPEQANIRVRLPKNPKGIFG
                     GKSVRGGVRFTLRIPATATLSSIETVNGSVTISAVSGRVDAKTVNGRIETRDLQGDAS
                     LETVNGSIATKFSRVSSHQEIALKTVNGSIDVSLPPDAGLKVRSSVVNGRISSDFPID
                     RATSREKKKLHGTIGDGRAALQAESVNGSIELAKL"
BASE COUNT          234 a          388 c          300 g          194 t
ORIGIN      
        1 cttcgccgtc gtcccgctcc tgctctccgg catcgccgcc gtcgcctgtc tgcttcccgc
       61 ccgccgcgcg acgcgcgtag atcccatggt cgcgttgcgc gccgaataac ggcgccatcg
      121 cgcggccgca gcttcttcca ggaaacgagg ggcgcaaaca ggcacaaaaa aacgctgaac
      181 cgaccggccg ttgcgccttc cactgtaatt cgcatcgaag gttgaagttg aattcgaaga
      241 ccccgcgcaa ccttcgtgta acttctcgcg cgcccgtcgt gttagtcctc cgtctcaatc
      301 actccatgaa aactcatctc ctctcccttc tcctcttcgg attcgtcgcc gctgcaccgg
      361 cgagcttgtt ctcggccacc gaatcattct cccgcagcgc ccccttcgat ccgaccggca
      421 aggtctcact gcacaatgtc aatggtgacg tgaccgtcga aacgtgggac cgcaacgaga
      481 tcgccatcga gggcgaaaag aacgcccgca ccgaagagga actgaaacgc atcgatctcg
      541 cgatcgagct cctgcctgaa caggccaaca tccgcgtgcg cttgccgaaa aatcccaaag
      601 gcattttcgg cggcaaatcg gtgcgcggtg gcgtgcgctt cacgttgcga atcccggcga
      661 cagccacgct ctcgagcatc gaaacggtga acggatccgt gacgatctcg gcggtcagcg
      721 gccgcgtgga cgccaaaacc gtcaacggcc gtatcgagac gcgcgatctc cagggcgacg
      781 cgtcactcga aaccgtgaac ggcagcatcg ccacgaagtt ctcccgcgtc agctcccacc
      841 aggagatagc gttgaaaacc gtcaacggct cgatcgacgt ttccctcccg ccggacgccg
      901 gactgaaagt gcgcagctcg gtggtcaatg gtcgcatctc ttccgatttt cccatcgatc
      961 gcgccaccag ccgcgaaaag aaaaaactcc acggcacgat cggcgacggc cgcgccgcac
     1021 tccaggcgga atcggtgaac ggaagcatcg agctcgcgaa actctaaacg cctacgtcat
     1081 accacggttc cgaccgacct ccgtgaaggt agggac
//
LOCUS       DASPLT010001287         2128 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_281173, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001287 DASPLT010000000
VERSION     DASPLT010001287.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 2128)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2128)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2128
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_281173"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1927
                     /locus_tag="VEA63_13150"
     CDS             <1..1927
                     /locus_tag="VEA63_13150"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HYD85002.1"
                     /translation="TPSFERKRFEALFTDYERQVHAQTHQLFWWLFLAQWAAAALIAA
                     VWSPRTWAGVDSAIHPHLIAAVAIGGLLVIPPLFLIRSMPHAWLTRHVVAVAQLSFSA
                     LLIHLSGGRIETHFHVFGSLAFLALYRDWRVMISATLVVTLDHLLRGLWFPESVYGVP
                     FATAWRTVEHAGWVIFEVLVLTWACIVSRREMGEICRREEQNQQLLEQLEQRVRDRTA
                     ALEAEVHQHECTTAELRLSEQRYRTLVENAPIGIFSSTRAGEVRFANPYLLRLVGLPV
                     DTPFASLNLSAGHIMRQESRDLFWSRLEADGEVRGFETVYLRPDGTRVEVVLNGQLTG
                     TTDGSPAVCEGTVEDVTARNRAKRDLEKVHHQFVVASRQAGMADVATGVLHNVGNVLT
                     SVNVTTQDLLERLRNSRLSMLGRIVNTIQRERPQLATFLTENSSGRRLPELLEKLDEH
                     LNQENAAVRRDVESLVRHFTHIREVIVTQQSTARLFGVREDLDPSHLFEDALRLITDS
                     LSRHGVTLERDFSPTPVMQGDRHKVLQILVNLIKNAKDSVLAAQMPLPKIIVRVAPGD
                     AGFVALSVEDNGLGIPEENLAKIFSHGFTTKKDGHGFGLHSCVLSAREMKGDLSVASA
                     GAGCGATFTLTLPATAP"
     gene            1924..>2128
                     /locus_tag="VEA63_13155"
     CDS             1924..>2128
                     /locus_tag="VEA63_13155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD85003.1"
                     /translation="MNRPALPKHRVLVIDDNVAIHEDFRRILSPAAIAPDLDAEAEAF
                     FGEPVPPGPESAGAIFELEFALQG"
BASE COUNT          380 a          681 c          656 g          411 t
ORIGIN      
        1 tacgccttcc tttgagcgga aacgtttcga agcgctgttc accgactacg aacggcaggt
       61 gcacgcgcag acccaccagc ttttctggtg gctgttcctg gcgcaatggg cggcggcggc
      121 cttgatcgct gccgtgtggt cgccgcgcac ctgggccggt gtcgacagtg ccattcatcc
      181 ccacctgatc gcggcggtcg ccatcggcgg attgctcgtg atcccgccgc ttttcctgat
      241 tcgttccatg ccgcacgcgt ggctcacgcg gcatgtggtg gccgtggccc agttgagctt
      301 ttccgccttg ttgatccatc tttcgggcgg tcgcatcgag acgcactttc acgtgttcgg
      361 atcgctggcg tttctcgcgc tttatcggga ctggcgcgtt atgatcagcg cgacgctggt
      421 cgtcaccctg gatcacctgc tgcgtggact ctggtttcca gaatcggtct acggtgtgcc
      481 gttcgcaacc gcatggcgga ccgtcgagca cgcgggctgg gtgatcttcg aagtcctcgt
      541 gctgacctgg gcctgcatcg tatcgcgtcg cgagatgggc gaaatctgcc ggcgtgaaga
      601 acagaaccag caattgctcg aacaactcga acaacgggtg cgcgaccgga ccgcggcgct
      661 cgaagcggaa gtgcaccagc acgaatgcac gaccgcggag ctgcgattga gcgagcaacg
      721 ttatcggacg ctcgtcgaga acgccccgat cggaattttc tcgagcacgc gcgccggcga
      781 agtgcggttc gcgaatccct acctgctgcg cctggtcggc ctgccggttg atacgccgtt
      841 cgcgtcgctc aacctttccg ccggccatat aatgaggcag gaaagtcgcg atctgttttg
      901 gtcgcgcctg gaggcagacg gagaagtgcg cggcttcgaa accgtctatc tgcgccctga
      961 cggcaccaga gttgaagtcg ttctcaatgg tcaattgacc gggaccacgg acggatctcc
     1021 ggcggtatgc gaagggaccg tcgaggacgt gaccgcccgg aatcgcgcca agcgcgacct
     1081 cgagaaggtt caccaccagt ttgtcgtcgc ctcgcgccag gccggcatgg ccgacgtcgc
     1141 gaccggcgtg ctgcacaacg tcggcaacgt gctcaccagc gtcaatgtca cgacccagga
     1201 tttgctcgaa cgcttgcgca acagccgact cagcatgttg ggccgaatcg taaacacgat
     1261 ccagcgcgag cgaccgcagc tcgcgacgtt ccttaccgag aattcgagcg gacggcgcct
     1321 ccctgaactt ctcgaaaagc tggatgagca cttgaaccag gagaacgccg ctgtgcgtcg
     1381 cgatgtcgaa agcctcgtcc gccacttcac gcacatccgc gaggtcatcg tgacgcagca
     1441 atccacggcg cgcctcttcg gcgtcaggga agacctcgat ccctcccatc tgtttgagga
     1501 cgcgctgagg ctgatcacgg attcgctcag ccggcatggc gtcacgctcg aacgcgactt
     1561 ctcgccgacg ccagtgatgc agggcgatcg ccacaaggtt cttcagattc tcgtcaacct
     1621 gatcaaaaac gcgaaggact cggtcctcgc cgcgcagatg ccattgccga aaatcatcgt
     1681 gcgggtcgca ccgggcgacg cgggttttgt tgcactttca gtcgaggaca acggcctcgg
     1741 catccccgag gagaatcttg cgaaaatctt ctcccacggt ttcaccacga agaaagacgg
     1801 gcacggcttc gggctgcaca gctgcgtgct ctccgcgcgg gaaatgaagg gcgatcttag
     1861 cgtggcgagc gccggcgcgg gttgcggcgc gacgttcact ctcaccttgc cggccaccgc
     1921 gccatgaacc ggcccgcgct tccgaagcat cgcgtgctgg tgatcgacga caacgtcgcc
     1981 attcacgagg actttcgccg gatcttgagc cccgcggcga tcgcgcccga tctcgacgcc
     2041 gaagcggagg cttttttcgg ggaaccggtc ccgcccgggc ccgagtcggc cggagctatt
     2101 ttcgagctcg aatttgcact ccaggggc
//
LOCUS       DASPLT010001288         1136 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_96463, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001288 DASPLT010000000
VERSION     DASPLT010001288.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1136)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1136)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1136
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_96463"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..417)
                     /locus_tag="VEA63_13160"
     CDS             complement(<1..417)
                     /locus_tag="VEA63_13160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD85004.1"
                     /translation="MMKKILLTVAAVALTGAVALAGGQAQTPATYAIRGAKIVPVSGA
                     VIESGNIVLRNGLIEAVGASAAIPPDAVIIEGKGLTVYPGLIDMGNAAPIEQPRGQQQ
                     QQGGFFGTPAEPGQSREDQERDKRERILRANYMATEH"
     gene            complement(425..>1136)
                     /locus_tag="VEA63_13165"
     CDS             complement(425..>1136)
                     /locus_tag="VEA63_13165"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HYD85005.1"
                     /translation="GGDRTYDDLKKDRDEKLARVSDYFARARAYAKAGEGKQTDWMLE
                     ALVPIVEKRTKLYTRVNTYLDFDDALQFAETENVDLVIITNPFNAATGVDTLKGKNVS
                     VILSEVQSLPQSDDVSHNFNYAAADVLADAGVKFAFSTGDYSNTRLLPYQAAQSVAWG
                     LSREEALKALTINAAEILGVGAAVGSLEEGKIANLFITDGDPLEVRTNVKNVIINGRN
                     VSLENKHLLLWQRFLGRQ"
BASE COUNT          159 a          374 c          366 g          237 t
ORIGIN      
        1 gtgctccgtg gccatgtagt tcgcgcgcag gatgcgctcg cgcttgtcgc gctcctgatc
       61 ctcgcgcgac tggccgggtt ccgccggcgt gccgaagaac ccgccctgct gttgctgctg
      121 tccgcgcggc tgttcgatgg gcgcggcgtt gcccatgtcg atcagccccg gatacaccgt
      181 gaggcccttg ccttcgatga tcacggcgtc gggcggaatc gcggcgcttg cgccgaccgc
      241 ttcgatcagc ccgttgcgca ggacgatgtt gccgctctcg atgacggcgc ccgaaaccgg
      301 aacgatcttc gcgccgcgga tggcgtaggt agccggcgtt tgcgcctgcc cgcctgcgag
      361 cgcgacggcg ccggtaagag caaccgctgc gactgttagc agaatctttt tcatcatcgc
      421 ctccctactg ccgcccgagg aaacgctgcc agagcagcag atgtttgttc tcgaggctga
      481 cgttccttcc gttgatgatg acgttcttca cgttcgtccg cacttcgagc gggtcaccgt
      541 ccgtgatgaa caggttggcg atcttgccct cctcgaggct gccgaccgcg gcgcccacgc
      601 cgaggatctc ggcggcgttg atcgtcaacg ccttgagggc ctcttcgcgc gacagccccc
      661 aggccaccga ctgtgccgcc tgatagggca gcagccgggt gtttgaatag tcgcctgtcg
      721 agaacgcgaa cttcacgccc gcgtcggcca gcacgtcggc tgccgcgtag ttgaagttgt
      781 gcgacacgtc gtcgctctgc gggagcgact gcacttcgct gaggatcacc gacacgttct
      841 tgcccttcag cgtgtcgacg ccggtcgcgg cgttgaacgg gttcgtgatg atcaccaggt
      901 cgacgttctc cgtctccgcg aactgcagcg cgtcgtcgaa gtcgaggtac gtgtttacgc
      961 gcgtgtagag cttcgtgcgc ttttcgacga tgggcacgag cgcttcgagc atccagtcgg
     1021 tctgcttgcc ctcgccggcc ttcgcgtagg cgcgggcgcg cgcgaagtag tcggagacgc
     1081 gcgccagctt ctcgtcgcgg tctttcttca aatcgtcgta ggtgcgatcg ccgcca
//
LOCUS       DASPLT010001289         1016 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_96468, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001289 DASPLT010000000
VERSION     DASPLT010001289.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1016)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1016)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1016
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_96468"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            255..>1016
                     /locus_tag="VEA63_13170"
     CDS             255..>1016
                     /locus_tag="VEA63_13170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007363427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HYD85006.1"
                     /translation="MKVHKLGIIMNGVTGRMGTNQHLIRSIKAIIDQGGVKLSDSEII
                     MPDPVLVGRSEDKLRALAARTGVSRITTNLDAELANPANQIYFDATLTGQRPIGVRKA
                     IAAKKHIYCEKPTATTSTEALALAKEVSAAGLKNGVVQDKLWLPGLLKLKYLIDTGFF
                     GKILSVRGEFGYWVFTGEHEKLQRPSWNYRKEDDGGIIVDMLCHWQYVIQNLFGDVKS
                     LTCLGATHVPERWDEAGKKYKCTADDAAYATFELTN"
BASE COUNT          221 a          343 c          262 g          190 t
ORIGIN      
        1 aggctgcatt ccccttcgcg aaattcgctc ctgggtcgag gccgccggct tcagcggctt
       61 caacgaagtc gagatctttt ccaaccgcca ctgggcccgc gaccaggacg aatttctccg
      121 cgacatcgtc tccgcttacc aaacccacgt ctgattcatc gccacgaaaa ggcacaaaaa
      181 cgcacgaaga atcccctgtc tcctgcctgt ttttcgcgcc ttttagtgcc tcttcgcggc
      241 cacctctcct caccatgaaa gttcacaaac tcggtatcat catgaacggc gtcacggggc
      301 gcatgggcac caaccagcat ctcatccgct cgatcaaagc gatcatcgat caaggcggcg
      361 tgaagctctc cgactccgaa atcatcatgc ccgacccggt gctcgtcggt cgcagcgagg
      421 ataaacttcg cgcgctggcg gcgcgcaccg gcgtctcacg catcaccacc aacctggatg
      481 ccgagctcgc gaatcccgcg aaccagatct acttcgacgc cacgctcacc ggccagcgcc
      541 cgatcggcgt gcgcaaggcg atcgctgcga agaaacacat ctattgcgag aaaccgacgg
      601 ccaccacttc caccgaagcg ctcgcgctcg cgaaggaagt ttccgccgcc ggcctgaaaa
      661 acggcgtcgt gcaggacaag ctctggcttc ctggtttgct gaagctcaag tatctcatcg
      721 ataccggatt cttcggcaaa atcctctccg tgcgcggcga gttcggctac tgggtcttca
      781 ccggcgaaca cgagaaactc cagcgcccgt cgtggaacta tcgcaaggaa gacgacggcg
      841 gcatcatcgt cgatatgctc tgccactggc agtatgtgat ccagaatctc tttggtgacg
      901 tgaagtcgct gacctgcctc ggcgccacgc acgtccccga acgttgggat gaagccggca
      961 agaaatacaa gtgcaccgcc gatgacgccg cgtatgcgac gttcgagctc accaac
//
LOCUS       DASPLT010001290         2869 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_96493, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001290 DASPLT010000000
VERSION     DASPLT010001290.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 2869)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2869)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2869
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_96493"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..975)
                     /locus_tag="VEA63_13175"
     CDS             complement(<1..975)
                     /locus_tag="VEA63_13175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HYD85007.1"
                     /translation="MILLYLAFAAVCSAASSPARGLPFTRRYSLEDIGYGPRGARLDF
                     DRFGRVAVIHDGVYAVLNDTIWSNIADRNPDRVTMSNVISAGDGHMYYGARASWGVAE
                     TQSDGLLHTRPLVPSNPPDWIRSASFADLIATEDGVYFVSPNGIAFWDFRAKETQLYE
                     HPRISRAFRVGNRVFTSSFNLDLRYVDIANREIVSVTNSNLDGVVELAAPLGEHRTLL
                     SLIDGRVVVFDGNKSAPWEPQTQPWGPDIRDGFGGKVAVLHRLVDGTIALGITGKGLY
                     LFSGDGESLLSLTSSEYHRITAIANREPGVLWVATEDGIEKILYSSALT"
     gene            1377..2639
                     /locus_tag="VEA63_13180"
     CDS             1377..2639
                     /locus_tag="VEA63_13180"
                     /inference="COORDINATES: protein motif:HMM:NF012742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HYD85008.1"
                     /translation="MAGSIPTSSRKVLLAAPAAPADLLRGVARYAREHKWHLITDMVV
                     TGAWPGEWSGDGILAAMPCHPEALTSVVEKGIPCVALSGAENAGVLPAVGPDNREIGR
                     IAADHLIERAHRSFAAVPFIDDAENQERLDAFRARVAEHGCDCTSFLPMYARAGCYSN
                     HNWAEHRRALIAQIERLPRPSAIFAFNDCVAAEIVDACREAGLLVPKDLAVLGVGDAI
                     VCTTSAVPISSIDTDLDEIGYRAAAVLEAAMNGVDVPAGVIRVPPKGVVTRMSTDLSA
                     VSDPRIARVLNYIAEHYPNPALSVGSIADAVGMSRRNLERSFREATGGSVHEHIVDVR
                     MREASRLLKSCPTTRNSYLAALVGFSGERTFFRVFRRYFGMSPKAHGDWANRARAANR
                     AVSLPVQRPAASVKSRRSPIEALPPTAA"
BASE COUNT          544 a          867 c          923 g          535 t
ORIGIN      
        1 cgtcagcgcg ctgctgtata atattttctc gatgccgtcc tcggtggcga cccacaacac
       61 gcccggctcc cggttcgcga tggccgtgat gcgatgatac tcggagctgg tcagcgagag
      121 aagcgattcg ccgtcaccag aaaacaaata cagacctttg ccggtgatcc ccagggcgat
      181 ggtgccatcg accaggcgat gcagcacggc cacttttcct ccgaagccgt cgcgaatgtc
      241 aggtccccag ggctgggttt gcggctccca cggcgcggac ttgtttccgt cgaacaccac
      301 gacgcgtccg tcgatcagcg aaaggagcgt gcggtgttcg ccgagcggag cggcgagctc
      361 gaccacgccg tcgagattgg agttggtgac gctgacgatc tcgcggttgg cgatatcgac
      421 gtaacgcaga tcgagattga acgaggaggt gaaaacccgg ttgccgacgc gaaaggcccg
      481 cgagattctc ggatgctcgt agagttgagt ttctttcgcg cgaaaatccc agaacgcgat
      541 gccgttcggg ctcacgaaat aaactccgtc ttcggtggcg atgagatccg caaacgacgc
      601 ggaacgaatc cagtcgggcg gatttgaagg gacgagtggc cgcgtgtgca gcaagccgtc
      661 ggattgcgtt tccgccacgc cccaggaggc gcgagccccg taatacatgt gcccgtctcc
      721 cgcagagatg acattcgaca tcgtgacacg atcgggattc cggtcggcga tgttggacca
      781 gatcgtgtca ttgagaactg catacacgcc gtcgtgtatc acggcgacgc gaccgaagcg
      841 gtcgaagtcc agtctcgccc cgcgcggccc gtagccgatg tcttcgagcg agtagcggcg
      901 cgtgaacgga agaccccgcg ccggagacga tgccgccgag cacacggcgg cgaacgccag
      961 atacagaagt atcacggcgc ggagcagagg gagtgagcgc gcagctttca ccgtcgaaat
     1021 gcccgtgctt tccggccgac gtagttgcat tcgagcgggg tcattcggat tgattcggat
     1081 gctgcgtgga acgtgaacgc tccgatcagg cgccgtaaag accaattggc acggacgccg
     1141 gaacaggggc cttttgcgtc aaccacgccg catattaccg gagtaataga acggggtatc
     1201 gccaatgctt cgttgccgca cggctgcggt taattcgttt tcggcgacgg ttcgaaaacg
     1261 gaatcgcgat ccgatccgta agttaccccc aaatgagttg ccccagaaca ctcgtgaacc
     1321 ggcttggtgc cggagattct cccgccgcac aggtcggaat gaggcggtga acaaccatgg
     1381 ccggttcgat tccaacgtct tcgcgaaaag tgctgctcgc cgcgccggcg gcgccggcgg
     1441 acctgctgcg cggcgtcgcc cggtatgcgc gggagcacaa gtggcacctg ataacggaca
     1501 tggttgtgac gggtgcctgg ccgggcgagt ggagcggcga tggcatcctc gcggccatgc
     1561 cgtgccatcc cgaggcgctg acgagcgtag ttgaaaaagg aataccatgt gtcgcgctct
     1621 ccggcgcgga gaacgccggc gtccttccgg ccgtcgggcc tgataatcgc gagatcgggc
     1681 gaatcgcggc cgatcatctg atcgaacgcg cgcaccgcag ttttgccgcg gtgcctttca
     1741 tcgacgatgc cgagaaccaa gagcgactcg acgcgttccg cgcgcgcgtc gcggaacatg
     1801 ggtgcgactg cacgagcttc ttgccgatgt atgcccgtgc cggttgttac tcgaatcaca
     1861 attgggcgga acatcgccgc gcgctgatcg cgcagatcga acgcctgccg cgcccctccg
     1921 cgatcttcgc gttcaacgac tgcgtcgcag ccgagatcgt cgatgcctgc cgcgaagcgg
     1981 gacttttggt tccaaaagac ctcgcggtgt tgggtgtggg cgatgccatc gtgtgcacca
     2041 cgtcggcggt gccgatctcg agtatcgata ccgatctgga tgagatcggc taccgggctg
     2101 cggcggttct ggaggcggcg atgaacggcg tggatgtgcc tgccggggtg atccgggtcc
     2161 cgcccaaagg agtggtgacg cggatgagca ccgatctaag tgcggtctcg gatcctcgca
     2221 tcgcgcgagt gttgaattac attgccgaac actatccgaa tccagccttg agcgtgggtt
     2281 cgatcgcgga tgcggttgga atgtctcgcc gcaatctgga acgaagcttc cgcgaggcga
     2341 ccggcggtag cgttcacgaa catatcgtgg acgttcgcat gcgcgaagcg tcgaggctgc
     2401 tcaagtcgtg tccgacgacc agaaattcct atctcgcggc gctcgtcggc ttttcgggcg
     2461 agcgaacttt ctttcgtgta ttccggcggt atttcggcat gagccccaag gcacacggcg
     2521 actgggccaa tcgcgcgcgg gcggccaatc gtgcggtcag tctgccggtg cagcggcccg
     2581 cggcctccgt caaaagccgg cgttcgccga tcgaagcgtt accgcccacg gcggcgtaag
     2641 ccatggcgcg cggtgagcat gtcctctctt gcggcgagga caacgccgcc cgtaaccaat
     2701 cggcgaatac atcagaatct tttcatgaga cgataccacc tccttgcctt cctgcttgtg
     2761 ctggccctgc cttcgttcgc gaacgcgcaa cccagctcgg tcgcatttga ccgcttcgac
     2821 tacaccggcc gcgacccggt cccgagcgaa acgctgcaac cgggcgagt
//
LOCUS       DASPLT010001291         7088 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_281240, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001291 DASPLT010000000
VERSION     DASPLT010001291.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 7088)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7088)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7088
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_281240"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            96..743
                     /locus_tag="VEA63_13185"
     CDS             96..743
                     /locus_tag="VEA63_13185"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HYD85009.1"
                     /translation="MSRTITPQRMRDEARALLRSMIVEGGLAPATHIEEVKLSQQIGA
                     SRTPVREALIALEGEGLVQSAPRKGFVVVEPNAALVRESFPILSALEGMAVRISGAAL
                     KTAAPQLRRINEALARERKKARQYDLDRAFHVALTKHCGNRRLLMLLEAERARAQLID
                     GAHQKGMANLKGSVEEHNEIIAALERGNADGAALVLARHWDNGINVVVKWLSEKS"
     gene            722..2329
                     /locus_tag="VEA63_13190"
     CDS             722..2329
                     /locus_tag="VEA63_13190"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HYD85010.1"
                     /translation="MALGKKLIAMATAVALVLVNGAAPAARAQDSDIVAWRALHEELA
                     IAGVLERLRAAPAPEIVILRNVSVVDPMERTVREGQSIVASLGRIYQVLPVPEEPQIE
                     GALIIDGGGRYAAPGLTDMHIHSTSASSYLLNIANGVTTVREMDGFPWMLAARDAVNN
                     DRMLGPTSYVAGTILNYVPMGGYAVVVQDAPTARRVVRQQAACGYDFIKIHNLMPQRV
                     FDTIAEEARRAGLDPVGHVPHFMSVRHAAERGMRTMEHLKGWLNDRTLTPGDTDFAAG
                     AVPDVWVTPTGYAYRNYAEAEAMEAMRTGPASRYVPARTRAQWSAFVETASTPVFALN
                     LNARPHRREIMRQLVSQGARFLAGTDAANYPFQTMGFALVEELRLLREAGVPEEQVFR
                     AATTEPAAAMRAERDFGRIAPGMRADIVLTTRNPLEGAHAAYSENQGVMLRGRWLELA
                     AIDGALAELADLYTDDQPAPRLTRASADAAARAAEALVESGFVFNPEILREAATVLRR
                     AGHNAAAGRLLALAIAPTSGPCAAITP"
     gene            2641..3012
                     /locus_tag="VEA63_13195"
     CDS             2641..3012
                     /locus_tag="VEA63_13195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD85011.1"
                     /translation="MLGVATMNVVQGGPWLSVQTFLLASGTFWVGAVAFIAPLSALIV
                     FVGRIGGIRRPWADTVGGAIAAVAGVFAADAVAELIFYGNLIPGDVMLVPFALIAGLV
                     AGPTYWWLAGRPQPPYMQAES"
     gene            complement(3105..3410)
                     /locus_tag="VEA63_13200"
     CDS             complement(3105..3410)
                     /locus_tag="VEA63_13200"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HYD85012.1"
                     /translation="MSRHYLRSSDFLFQSRLANSPHLSTRQYARIVQCRVVSIGLNPA
                     TYGTRSLRRTKAALIYWKTGNLRAVQLLLGHTKLESTVRYLGVEVDDAIAISEGVDL"
     gene            complement(3410..3655)
                     /locus_tag="VEA63_13205"
     CDS             complement(3410..3655)
                     /locus_tag="VEA63_13205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD85013.1"
                     /translation="MLYELLALSEMTIIRRYRTLAHAVEFDGEGPARNQADCEGKVED
                     IAAGGEVKTRGIHCPEENRPAGAIRNHRADQETRSGH"
     gene            3661..4632
                     /locus_tag="VEA63_13210"
     CDS             3661..4632
                     /locus_tag="VEA63_13210"
                     /inference="COORDINATES: protein motif:HMM:NF014068.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HYD85014.1"
                     /translation="MSKVLKVACLVFDGVNATDVAAPLEAFAAARDERGRPLYTPAIW
                     SVRQRTVRAESGLQLSADRMLPTRAGADLLIVPGGAGIREPQNLAGLSAWLLAHHRRF
                     PRIASICTGAYALAEAGIVNGNSIATHWRHASALQKRYPRVRVDADALFLKDGRFYSS
                     GGVTAGIDLALELIENDFGPRTAMDVARELVVFLRRTGTQNQFSAPLQVQTAASGRLR
                     DVCAWAAANLDDDLSVESLAKRAGLSARQFSRCFLEAFGAPPATYFKAMRLDASRTLL
                     EQGASIASVATASGFLSADVFRRAFERQYGVAPSDYRRGFMIKRGVR"
     gene            4863..5711
                     /locus_tag="VEA63_13215"
     CDS             4863..5711
                     /locus_tag="VEA63_13215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD85015.1"
                     /translation="MFLAAGGLGREAARIEVHYFLPRTFTPRSPVLLVIPGAGRDGDE
                     YRDAWIATADRVGALVASLGYPEAHYDFAAYQLGGVVRDLTLPAGSRDSNVVRIRDED
                     IVFTPNPESDTWLFNDFDRVFAVLKRAAGSDQVGYDMFGHSAGGQILHRLVLFHPHSR
                     AERIIAANAGFYTLPNLDLPMIVGMGGSGVGEIELAQAFGRPLTLLLGEADNGDDAGG
                     IMLHTPLVDQQGLGRLDRGRYFFEQAQQRARLLNEPLAWRLRTVPGVGHNFRAMSQAA
                     ASLLYP"
     gene            complement(5841..6332)
                     /locus_tag="VEA63_13220"
     CDS             complement(5841..6332)
                     /locus_tag="VEA63_13220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HYD85016.1"
                     /translation="MTDVTAQSAWSGFITTMSTTRRQIVALPIAAGLAALEAARRPNG
                     GSPMTMRRLFADENGAARFEDWRPELSSGTLGRRQLSLSTPLRASACFIIEAPAGGGH
                     PTQPEAGRRLVVMLSGECEVTASDETVVGRPGDLLLVEDTTGVGHSSRTASGFTALMI
                     VLD"
     gene            complement(6412..6750)
                     /locus_tag="VEA63_13225"
     CDS             complement(6412..6750)
                     /locus_tag="VEA63_13225"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HYD85017.1"
                     /translation="MSDVRQTAQWYERIGFTVSGTHDCGNGELDWARLTFGETAIMLN
                     IGGKPSQAERREVDLYLEVDQVDEAWSRVASIAEVVEPPHDTEYGMREFIVRDLNGFW
                     ITFGQQAKQV"
BASE COUNT         1248 a         2208 c         2223 g         1409 t
ORIGIN      
        1 gagttcggcg atcgtgagcc gttcagcgga gtagggatct gccggcggcg tcggagctag
       61 tttgcagcag cgcatccacc atgtatacat aatacatgag ccgaaccatc acgccccaac
      121 gcatgcggga cgaagcccgc gctttgctcc gctcgatgat cgttgagggc ggactagcgc
      181 cggcgacgca catcgaggaa gtgaaactgt cgcagcagat cggcgccagc cgcacgccgg
      241 tgcgggaggc gctgatcgcg ttggaaggcg agggcttggt gcaatctgcc ccgcgaaagg
      301 gtttcgtcgt cgtcgagccc aacgcggcgc tggtgcgcga gagctttccc attctttccg
      361 cgcttgaagg aatggccgtt cgcatttcgg gtgcggcgct caaaacagcc gctccacaac
      421 tccgccgcat caacgaagcg ctcgctcgtg agcgcaaaaa ggcgcgccaa tacgatctcg
      481 accgcgcttt tcatgtcgcg ctgacgaagc attgcggcaa tcggcgtttg ttgatgctgc
      541 tcgaagccga gcgcgcgcgt gcgcagctga ttgacggcgc acaccagaag ggcatggcga
      601 acctcaaagg ttctgttgag gagcacaacg aaatcatcgc cgcgctcgaa cgcggcaacg
      661 cagacggcgc ggcgctcgtg ctggcgcgcc attgggacaa tggcatcaac gtggtggtga
      721 aatggctctc ggaaaaaagc tgatcgcgat ggcgaccgca gtcgcgctcg tcttggtaaa
      781 cggcgccgcg ccagcagcac gcgcgcagga cagcgacatc gtcgcttggc gagcgctgca
      841 cgaagagctc gccatcgccg gcgtcctgga gcggttgcgc gcggcgccgg cgcccgagat
      901 cgtcattctg cgcaatgtaa gcgtggtcga tccgatggag cgcactgtcc gcgagggcca
      961 aagcatcgtc gcaagccttg gtcgcatcta tcaggtcctg ccggtacccg aagagccgca
     1021 gatcgaaggg gcgctcatca tcgacggcgg cggccgttac gcggcgcctg gcctcacgga
     1081 tatgcacatc cactccacct cggcctcctc ctatctgctc aacatagcca acggcgtgac
     1141 caccgtccgt gaaatggacg ggtttccgtg gatgctggcc gcgcgcgatg cagtgaacaa
     1201 cgaccgcatg ctcggcccga cctcgtatgt ggccggcaca atcctcaatt acgtcccgat
     1261 gggcggctac gccgtcgtgg tgcaagacgc tcccaccgcg cggcgcgtcg tccgccagca
     1321 agcggcgtgc ggctacgatt tcatcaagat ccacaacctc atgccgcagc gcgtgttcga
     1381 cacgattgcc gaagaagcgc gccgcgcggg gctcgatccg gtcggacacg ttccgcattt
     1441 catgagcgtg cggcacgccg ccgagcgggg catgcgcacg atggagcacc tcaaaggctg
     1501 gctcaacgat cgcacgctga cgcccggcga taccgatttc gccgccggcg ccgtgcccga
     1561 cgtgtgggtg acgccgaccg gttacgcata tcgcaattac gccgaagcgg aggcgatgga
     1621 ggccatgcgc acgggccccg cctcgcgcta tgttcctgcc cgcacgcgtg cgcaatggtc
     1681 ggcgttcgtg gaaacggcct cgacgcctgt ttttgcgctg aacctcaacg cgcgcccgca
     1741 tcggcgggaa atcatgcgtc agctggtaag tcagggcgcg cgtttcctcg ccggcacgga
     1801 cgcggccaat tacccgttcc agacgatggg ctttgcactc gttgaggagc tgcgcttgct
     1861 ccgcgaagca ggcgtgcccg aagagcaggt gttccgcgcc gccaccaccg agcccgccgc
     1921 cgccatgcgc gcggagcggg atttcggccg catcgcgccc ggcatgcgcg ccgacatcgt
     1981 tctcaccacg cgcaatccgc tcgaaggagc acacgcggcc tacagcgaga accaaggcgt
     2041 catgctgcgc ggccgctggc ttgagctcgc cgccatcgat ggtgcgcttg ccgagcttgc
     2101 agatctttac accgacgacc aacccgcacc gcgcctcacc cgcgccagcg cagacgccgc
     2161 ggcccgcgcc gccgaggcgc ttgtcgaaag cggtttcgtc ttcaatccgg agatcttgcg
     2221 tgaagccgcc accgtgctcc ggcgcgcggg gcacaacgcc gccgccggac gcctcctggc
     2281 actcgccatc gcgcccacaa gcgggccctg cgcggcgatc acgccgtagg ttcagttctg
     2341 atttgggagt ctcgaagcgt cgcggcagcg cgaacgccgc gcgatcagtt ttagcagcca
     2401 atcagtcact cagcctaaca ccgagaatga ccggttgcgg cgatcgttag ccgttcagcg
     2461 gagtagggcg cctccgcggc tttggagcca gcgagccagt cgcgaagatc gtctggccga
     2521 actacctgac ccggagcgga ccttgcgata ctgcgctagt atcgaggaat gttgaaaccg
     2581 agtcctcttc ggtcggccct ggcatgggcg ggagcatgtt taatcggcgt cgtcgttagc
     2641 gtgctcggcg tggcgacaat gaatgtcgtg caaggcggcc cctggctctc agtgcaaacg
     2701 ttccttttgg cgtcgggtac cttctgggta ggagcagtgg cgttcatcgc gccactctcc
     2761 gcgctgatcg ttttcgttgg tcgaatagga ggtatccgta ggccgtgggc ggacaccgtg
     2821 ggtggtgcta tagcggcggt cgctggcgtc ttcgcggcgg atgctgttgc cgaactgatc
     2881 ttttacggca atcttattcc cggtgatgtg atgctcgttc cttttgcgct gatcgctggg
     2941 ctagtcgcgg gtccgactta ctggtggctc gctggaagac cacaaccgcc atacatgcaa
     3001 gccgagagct gaggttgccg aacgtccaag ttcggcgaat ataggtcgcg ctgtcactcg
     3061 taggagaatg gccgatccta cgcgaacctg ccattccttt ctgatcagag atcgacgcct
     3121 tctgagatgg ctatcgcgtc atcgacctcg acaccaagat agcgaaccgt gctttccagc
     3181 ttggtgtgcc cgaggagaag ctgcaccgct cgtaggttac cggtcttcca atagatgagc
     3241 gccgctttgg tgcgccgcag cgagcgcgtc ccgtaagtgg ccgggttcag cccgatagag
     3301 accacccggc attgtacgat ccgcgcatac tgccgcgtcg agaggtgagg ggaatttgca
     3361 aggcggcttt ggaataggaa gtcgcttgac ctgaggtagt gccggctcat cagtgtccgg
     3421 accgcgtttc ctggtctgct cggtgatttc gaattgcacc ggccggccgg ttttcttctg
     3481 gacaatggat gccgcgcgtc ttcacctcgc cgccggcggc gatgtcctcg actttcccct
     3541 cgcagtcagc ttgatttcgt gccgggcctt ctccatcgaa ctctaccgca tgcgccagtg
     3601 tccgatatcg tcggataatt gtcatttcgg acagagccag cagctcgtaa agcactcagc
     3661 atgagcaaag tactcaaggt cgcttgcctc gtctttgacg gggtcaacgc caccgacgtg
     3721 gctgcgccgc tggaggcgtt tgccgccgcg cgagacgagc gcggccggcc actttacacc
     3781 cccgcgatct ggtctgttag gcagcgcacg gtacgcgcag agtctggtct tcaactcagc
     3841 gcggaccgca tgctgccgac gcgcgccggc gcggatttgc tcatcgttcc cggcggcgcc
     3901 ggcattcgtg aaccgcagaa cctggctgga ttatctgctt ggcttctcgc ccatcatcgc
     3961 cggtttccgc ggatcgcatc gatctgcacc ggcgcttacg cgctggccga ggccgggatc
     4021 gtaaatggca attccatcgc gacccactgg cgtcatgcaa gcgcactgca gaagcgctat
     4081 ccccgtgtgc gcgtcgatgc tgacgctttg tttctgaaag acggccgctt ctattcctcc
     4141 ggtggggtca cggccgggat cgatttggcg ctagagctga tcgagaatga ttttggtccc
     4201 cgaacggcaa tggatgtcgc gcgcgaattg gtggtgtttc tgcgccgaac cgggacccag
     4261 aaccaattct cggcgccgct ccaggttcag accgcggcgt cagggcgttt aagggacgtg
     4321 tgcgcctggg ccgcggccaa tcttgacgac gatctctccg tcgaatcttt ggcaaagcgc
     4381 gcgggcctta gtgcgcgcca attctcccgc tgctttctag aagcgttcgg cgcgcctccg
     4441 gctacatatt tcaaggcaat gcggctggat gcttcccgca cgttgctgga gcagggcgcc
     4501 agcatcgcct cggtcgcaac ggcaagcggc ttcttgagcg cggacgtgtt tcgacgcgcc
     4561 tttgagcgtc aatatggcgt cgcgcccagt gattaccggc gcgggttcat gatcaagcgg
     4621 ggcgtacgat gaaagagatg acgcgacgct cggttgcggc ctggggactg gcgtcggtct
     4681 gcgggtgtgc ggccaatggc cgaacttcga tattgtcagg tcggtttgtc tgtccaccgt
     4741 gcgggtgcgc gatggatggc gtagagttca gcgcgccggg cgtctgcccg gcatgcggca
     4801 tgagcttgac gccaaagcat gacgcgccct ttgagccgac ttcactttcg ccaggcgcgg
     4861 gcatgtttct cgccgcggga gggcttgggc gcgaggcagc ccggatcgaa gtgcattatt
     4921 ttctgccgcg aacgttcacg ccgcgctcgc cggtgttgct cgtaattccg ggtgcaggac
     4981 gcgacggtga cgaatatcgc gacgcctgga tcgcgaccgc cgatcgcgtc ggagcgctcg
     5041 tcgcatcgct cggatatcca gaggcgcact atgatttcgc ggcctatcaa ttgggcgggg
     5101 tggttcgcga tctgacgctg ccagcgggat cgcgagactc gaacgtcgtc cggattcgcg
     5161 atgaagatat tgtcttcacc cctaatccgg agtcggacac ttggctcttc aatgatttcg
     5221 atcgtgtctt tgccgttctg aaaagggccg cgggctccga tcaggtcgga tatgacatgt
     5281 tcgggcattc agccggcggc cagattctac atcgcctagt cctttttcac ccgcattcgc
     5341 gcgccgagcg gatcatcgcc gctaatgctg gcttctacac gcttccaaat cttgatttgc
     5401 cgatgattgt cggcatgggg ggaagcggcg ttggcgagat cgagctggcg caagcttttg
     5461 gtcgaccact tacgcttttg ctgggagaag cggacaacgg cgacgacgcc ggcgggatta
     5521 tgttgcatac gccgcttgtc gatcagcagg ggcttggccg tctggatcgg ggccggtact
     5581 tcttcgagca agcccaacaa cgcgcgcgtt tgttgaacga gccccttgcg tggcgtttgc
     5641 gaacggtgcc cggagtgggg cacaatttcc gcgccatgag tcaagctgcc gcttcattgc
     5701 tttacccgta gcgcgctgtg gtcgccccca tgtctgtttt cggcttcgca aggagggcga
     5761 tcgcttctgg tatcaagccg caagtttgac gacggtcagt gcgacgggat tttcgagagc
     5821 catgccgtcg atagataggc tcaatccagc acgatcatca gcgcggtaaa gccgctcgct
     5881 gtgcgactgg aatggcccac gccggttgtg tcctccacga gtaagagatc gccgggacgg
     5941 ccgaccacgg tttcatccga tgcggtgact tcgcactcgc ccgacagcat caccacaagc
     6001 cgccgtcctg cttctggttg cgtcggatgg cctccgcccg ccggcgcctc gataataaag
     6061 catgccgagg cccgtagtgg cgtggacaga ctcaattggc gacgccccag cgttcccgac
     6121 gaaagctccg gacgccaatc ctcgaaccgg gcggcgccgt tttcatcggc gaataatctg
     6181 cgcatcgtca tgggtgaacc tccattcggc cgacgcgccg cttccaacgc ggcaagtccg
     6241 gccgcgattg ggagggcgac gatctggcgt cgcgtcgttg acatagttgt gatgaacccg
     6301 ctccacgcgg attgcgccgt gacgtcggtc atgcaggcag gaaggtgaaa tggcccagcg
     6361 atttgctgcc tggctccaaa gggtgtcgtt ttcagcaaag cagcgcagac ttcaaacttg
     6421 cttggcttgc tgtccaaacg tgatccagaa gccatttagg tcgcggacaa tgaactcacg
     6481 catgccatac tcagtgtcat gcggcggctc gaccacctct gcgattgaag cgacgcgtga
     6541 ccacgcctca tcgacttggt ccacttccaa gtaaaggtcg acttcccggc gctctgcctg
     6601 cgagggcttg cctccaatat tcagcatgat cgcggtttcg ccaaatgtca ggcgcgccca
     6661 gtcaagctcc ccgttgccgc aatcgtgcgt cccgctgact gtaaatccaa ttctttcgta
     6721 ccactgtgct gtctggcgca cgtcggacac atggatcatc ggcgttatag accgtgacat
     6781 aagatgctcc tgagggaccg gctttgcatg tctgcacgaa aggctgctat cggaatgtgt
     6841 agacgtcgcg cgaaggctgc cgttgcgcgc cggtgcacaa aagcgcgctg aggacgccgc
     6901 gcagttcgaa gcgatgcaag tgcgctctgc atccattcta ccaaaatccc cagccgatcg
     6961 cgcgcgcatt gtcgactggt cgattgcgtc tgatcgggcg acactggccg ctgcaagcgt
     7021 ttggcccgtc agcagccccg ccaggcagcc gaacacttgc tcgtcggaaa gcgcctacga
     7081 tgcctccc
//
LOCUS       DASPLT010001292         1410 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_239488, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001292 DASPLT010000000
VERSION     DASPLT010001292.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1410)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1410)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1410
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_239488"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            191..>1410
                     /locus_tag="VEA63_13230"
     CDS             191..>1410
                     /locus_tag="VEA63_13230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD85018.1"
                     /translation="MMLRSRPVRYLITRVARAYGFLDPVALLAHLRGFAKPSEVAEPI
                     ELLRAGMLFHARGLVNTKAIQNNLDWVWPYWVERQFNPSDPSFIPRAFSFSHINLTHR
                     NWTAVGVPDVPYYPIVDPRGLVTPLFDGWSLDFWFLPSNGDPILLPSKLAEVEQELSL
                     SENLQVRSIAERDGVAIESVVTLTLDGGEPRCRIRVRAQGAGAGSIAVALRPYNPEGV
                     SFVKEIALLKDGRGWLVNGRNEVTFDCTPAKQLLSTYEAGDVLLRMNQDITASSVTCP
                     VSMATAVALFPVTGRSDESCEVIVPIYNELDPKRRPNFPSTQSWAESIAPLARLRVSD
                     PEVQRLYDLAIANLVLHAPREVYPGPYTYKRFWFRDAVFILNALLSLGGVERTRRAMG
                     EFAPRQRLDGYFLS"
BASE COUNT          232 a          425 c          461 g          292 t
ORIGIN      
        1 acgagcgcac cccctacagg cccgaacgat cagcaggcag gcacggaggc ctgctgctac
       61 gctgctgccg acgacgcgca tgcgagagtt gccctaattt ttagtggtga cgatttcggt
      121 cggcgggctg caaagcggca cttttgggtt taattgggga caatcgggtt ggaaccgacc
      181 cgcccgcccc atgatgctcc gctcacgccc agtccgttat ctgatcaccc gcgtggcgcg
      241 cgcctacggt tttctcgatc cggtggcgtt gctggcacat cttcgcggct tcgccaagcc
      301 gtcggaggtc gccgagccga tcgagctcct gcgcgccggg atgttgtttc acgcgcgcgg
      361 gctcgtgaac accaaggcga ttcaaaacaa tctcgactgg gtgtggcctt actgggtcga
      421 gcggcagttc aacccgtccg acccctcgtt cattccgcgc gcgttttcgt tcagccacat
      481 caatctcacg caccgcaact ggacggcggt cggcgtgccg gatgtgccgt attatccgat
      541 cgtcgatccg cgcgggctgg tgacgccgtt gttcgacggc tggtcgttgg acttctggtt
      601 cttgccgagc aacggcgatc cgattctgct tccttcgaag ctcgctgagg tcgagcagga
      661 gttgtcgctc agcgagaatt tgcaggttcg ttcgattgca gaacgcgacg gcgtcgcgat
      721 cgaaagcgtg gtcacgctga cgctcgatgg cggcgaaccg cgctgccgga tacgcgtgcg
      781 cgcgcaaggc gccggagccg gctcgatcgc cgtggcgttg cggccttaca atccggaagg
      841 cgtcagcttc gtgaaggaga tcgcgttgct caaagatggt cgcggctggc tcgtgaatgg
      901 acgcaacgag gtcaccttcg actgcacgcc ggcgaaacaa ctgctatcga cgtatgaggc
      961 cggcgacgtg ttgctgcgga tgaaccagga catcacggcg tcgagcgtca cgtgtccggt
     1021 gagcatggcg acggcggtgg cgctgtttcc cgtgaccggt cgcagcgacg agtcgtgcga
     1081 ggtgatcgtg ccgatctaca acgaactcga tccgaagcgc cgtccgaatt ttccaagcac
     1141 gcaatcgtgg gccgagtcga tcgcgccgct tgcgcgtttg cgcgtgagcg atccggaggt
     1201 gcagcgactc tacgatctcg cgattgcgaa tctcgtgttg cacgcgccgc gcgaggttta
     1261 tccagggccg tatacgtata aacgtttttg gtttcgcgac gcggtgttta ttctcaacgc
     1321 gctgctgtcg ctcggcggcg tggagcgcac gcgacgggcg atgggcgaat tcgcgccgcg
     1381 gcagcggctc gacggctact ttctttcgca
//
LOCUS       DASPLT010001293         1003 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_15923, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001293 DASPLT010000000
VERSION     DASPLT010001293.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1003)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1003)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1003
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_15923"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..984)
                     /locus_tag="VEA63_13235"
     CDS             complement(<1..984)
                     /locus_tag="VEA63_13235"
                     /inference="COORDINATES: protein motif:HMM:NF012686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD85019.1"
                     /translation="MSTASLVYQQGVSGAWLSFQMVFMNPYFWFMNLWFRRVRLVTMA
                     DLLEQRMNSRRLAQFYAVLQLLVAVIITVGFGNLVTYKISTAVVVKPEASWTEAERQS
                     VEGYHSWRKLEQAAAAGVLDAVQQSQLDVLRERAARDELRDTISPFEPLPFYLLYTLV
                     IGAYILMGGMTATAINEMVQCVLILVFSSMLLPVGLDAIGGFDALAERVPRQMFEFFG
                     EAGVSQVTGLTVFAVFFVSWVQITGIMGNMTIGGSARTEFAARFGAVAGTYAKRFMVI
                     LWSFCGLVAYALYSGALSDADMVWGAMSRDLLGPGLLGVMLAGVLAANMSSV"
BASE COUNT          224 a          344 c          290 g          145 t
ORIGIN      
        1 gaccgaggac atgttcgccg ccagcacgcc cgcgagcatc acgcccagca ggccgggccc
       61 cagcagatca cgcgacatcg cgccccacac catgtccgcg tcggacaatg caccggaata
      121 aagcgcgtag gcgacgagtc cgcagaacga ccacagaatc accatgaacc gcttggcgta
      181 agtgccggcg accgcaccga aacgcgcggc gaactccgtg cgcgccgacc cgccgatggt
      241 catgttgccc atgatcccgg tgatctgcac ccacgacacg aaaaagaccg cgaacaccgt
      301 cagccccgtc acctggctca cgcccgcctc gccgaaaaac tcgaacatct gccgcggcac
      361 gcgctccgcg agcgcgtcga aaccgccgat ggcatccaat ccgacgggca gcagcatcga
      421 ggagaaaacg aggatcagca cgcattgcac catttcgttg atcgcggtcg cggtcatgcc
      481 gcccatcaga atatacgcgc cgatcaccag cgtatagagc aggtaaaacg gcagcggttc
      541 gaacgggctg atcgtgtcgc gcaactcgtc gcgcgccgcg cgttcgcgca gaacatcgag
      601 ctggctttgc tgcacagcat cgagcacgcc agcagccgcg gcttgctcga gctttcgcca
      661 cgaatgatag ccctcgaccg actggcgctc ggcttcggtc cagctcgctt ccggcttcac
      721 gacgacagcc gtggagatct tgtaggtgac gagattgccg aagcccaccg tgatgatgac
      781 ggccacgagc agttgaagca ccgcgtaaaa ctgcgcgagc cgccggctgt tcatccgctg
      841 ctcgagcaag tcggccatcg tcacgagccg cacgcgacgg aaccagagat tcatgaacca
      901 aaaatacggg ttcatgaaaa ccatctggaa ggaaagccac gcgccggaca cgccctgctg
      961 atacacgagg ctcgccgtgc tcacggcgtt gttcgcgtcg gtg
//
LOCUS       DASPLT010001294         1818 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_302711, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001294 DASPLT010000000
VERSION     DASPLT010001294.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1818)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1818)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1818
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_302711"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..700)
                     /locus_tag="VEA63_13240"
     CDS             complement(<1..700)
                     /locus_tag="VEA63_13240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD85020.1"
                     /translation="MKNTPKSCPGMTAFIAVIGLCLISAVPVNATPVPATEAERFVAS
                     TAVATHWRFNNVYTNYAELSELLIDSGIRHSRDAGSSGNFISKIQKLSDAGVKSIGMV
                     LPSAGVRPNGSYWANSPVYNIDDYVHAVGRDVIAFVEMNNELDMTSQQANTYWHNLSE
                     PLSNDPNSPYYYADYAQAATADTWTQLISHSDPSIASLPLIGPSLVSDAAYTTVGDLS
                     DSIEYSNIHHYLYGW"
     gene            complement(946..>1818)
                     /locus_tag="VEA63_13245"
     CDS             complement(946..>1818)
                     /locus_tag="VEA63_13245"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HYD85021.1"
                     /translation="EKRRAEFYPQTRVCSSVAELLATPGLDVVDIATHAGVRGPLIEQ
                     AIAAGKHVLSQKPFVLELAEGRRLAALAAAAGVKLAVNQNGRWAPAFAYLRQAVRAGL
                     IGRISSVAIRINWDHTWTAGTAFEQMQHLVLYDFAIHWFDAAYSFFGGEAARTIYATV
                     ASAPDQPIKPPLVASSVVTFPSGIATLSFNGCSRHAPSESCTIVGSLGTLHATGGVCA
                     MPAVELTTAAGHARAELRGSWFPDGFRGAMGELLCAIEEDRAPENSAEDNLKSLAICL
                     GALRSADEGRIVEL"
BASE COUNT          342 a          501 c          645 g          330 t
ORIGIN      
        1 tccatccata aagataatga tgaatgttac tgtattcgat actgtcgctg aggtcaccga
       61 ccgtcgtgta ggcggcgtcg gagacgagag agggaccgat gagaggcagg ctcgcgatcg
      121 acggatcgct gtgactgatc aactgggtcc acgtgtccgc cgtcgcggcc tgcgcgtagt
      181 cggcgtagta gtagggcgag ttgggatcgt tcgagagcgg ttcgctgagg ttgtgccaat
      241 atgtgttggc ctgctgcgaa gtcatatcga gttcgttgtt catttcgacg aacgcgatga
      301 cgtcgcgccc gacggcgtgc acataatcat cgatgttgta gacgggagag ttcgcccaat
      361 aagagccgtt ggggcggacg ccggcggaag ggagcaccat cccgatgctt ttcacgccgg
      421 cgtcggatag tttttggatt ttcgaaatga agttaccgct ggatccggcg tcgcggctgt
      481 gacggatgcc ggagtcgatc aagagctcgc tgagctcggc gtagttcgtg tagacgttgt
      541 tgaatcgcca gtgggtcgcg accgcggtgg aagccacgaa acgctcggct tcggtggcgg
      601 ggacgggcgt agcgttgacg gggacggcgc tgatgaggca aaggccgatg acggcgatga
      661 acgccgtcat gccggggcat gactttgggg tgtttttcat gggagcggta acggtgccga
      721 agctccgggg tggggcggtc ggcgggtaat cggaaaagcc gggtgtgccg gcgccgttac
      781 agatcctgtc tgttcccgcg gcggcaattc accctgcgtc gaaggcgcgc gatcgattcc
      841 ataagtggaa tcgcggcatg cgtgacgcgc gccctaaaag gctatcgagc caaaacgagg
      901 gacacagtcg cacgagcggc cgtatccaac cgaccaatat ccggtctata gctcgacgat
      961 gcggccttcg tcggcagagc ggagcgcgcc gaggcagatc gcgaggctct tcaggttgtc
     1021 ctcggcggaa ttctcgggtg cgcggtcttc ttcaatcgcg caaagcaact ctcccatggc
     1081 tccgcggaaa ccgtcgggaa accacgatcc gcggagctcg gcgcgcgcat ggccggcggc
     1141 ggtggtgagc tcgacggcgg gcattgcgca gactccgccg gtggcgtgga gggtgccaag
     1201 actgcccacg atcgtgcagc tttccgacgg cgcgtgacgg ctgcagccgt tgaacgacag
     1261 cgtggcgatc ccggaaggaa acgtcacgac gctcgacgcc acgagcggcg gcttgatcgg
     1321 ctggtcgggc gcgctcgcga cggtcgcgta gatggtgcgc gcggcttcac cgccaaaaaa
     1381 cgaatacgcg gcgtcaaacc agtgaatggc gaagtcgtag agcacgaggt gctgcatctg
     1441 ctcgaaggcg gtgcccgcag tccaggtgtg gtcccaatta atcctgatcg cgaccgagct
     1501 gatgcggccg atcaaaccgg cgcgcaccgc ctggcgaaga tacgcgaaag caggagccca
     1561 gcggccattc tggttgacgg ccagcttgac gccagcggcg gcggcgagag cggcgaggcg
     1621 gcgaccctcg gcgagttcga gcacgaaggg tttttgcgag aggacatgtt tgcccgcggc
     1681 gatggcctgt tcgatcaacg gtccgcgaac gccggcgtgc gtggcaatat cgaccacgtc
     1741 gagcccgggc gtggcgagca gttcggcgac gctcgagcag acgcgcgtct gcggataaaa
     1801 ttcggcgcga cgtttttc
//
LOCUS       DASPLT010001295         2254 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_117085, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001295 DASPLT010000000
VERSION     DASPLT010001295.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 2254)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2254)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2254
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_117085"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..195
                     /locus_tag="VEA63_13250"
     CDS             <1..195
                     /locus_tag="VEA63_13250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD85022.1"
                     /translation="AWDNRPSAPTPAHARAEKDFINRVAVFVDRCGRHELQRDAFAFY
                     KQTVTDFFARYESEEILGRR"
     gene            341..985
                     /locus_tag="VEA63_13255"
     CDS             341..985
                     /locus_tag="VEA63_13255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6250 domain-containing protein"
                     /protein_id="HYD85023.1"
                     /translation="MILFRLLVLLCITSAVQAASSPTADLSSWSVEQQPGGTVTVDGD
                     ALVIEDAGGCTVWFRQKLTAPVEISYEVTAISRGGPHDRVSDVNCFWMASDPRSPETL
                     PEGRSGKFADYDSLRTYYVGMGGWNNTKTRFRRYAGAGVKPLLPEHDLSDAQFLLVPN
                     KTYRIKLVARDGLAEFWRDGEKIFSFRDPEPLTSGWFGVRTVKSHLEIRNLRIK"
     gene            complement(992..1558)
                     /locus_tag="VEA63_13260"
     CDS             complement(992..1558)
                     /locus_tag="VEA63_13260"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HYD85024.1"
                     /translation="MSSLVRILAFSGSARRESFNRKFLAVAVEQVKEAGGEVTLVDLN
                     DYALPIYHGDLEDAEGLPENAKKLIALISEHPALLIASPEYNSMITPLLKNTIDWCTR
                     ADDNPFIGKVAAVVSASPGMFGGVRSLKMAQQLLQHLGCHVVPGQVILAHANKAFDAA
                     GKLNEPRTLKSVTDLAERLVATTRKLAG"
     gene            complement(1653..>2254)
                     /locus_tag="VEA63_13265"
     CDS             complement(1653..>2254)
                     /locus_tag="VEA63_13265"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HYD85025.1"
                     /translation="HFSYAREFAAVHPAVRIHPERVLVISGTREELVTSGASMTWHDL
                     VLYLIARYAGATAAQGVARAFALQWHQDGLAPYIAFEGRIDHGDGEIQAAQQWLAAHF
                     SVANCVGEVIKRSRLAERTFKRRFTVATGLAPLAYVQRLRIEDAKRRLERTTSSVDEI
                     GWQVGYEDPAFFRRLFKRATGIAPGAYRKRFRILDFARM"
BASE COUNT          443 a          774 c          612 g          425 t
ORIGIN      
        1 gcgtgggaca accgccccag tgcgcccacg cccgcgcacg cccgcgccga aaaagacttc
       61 atcaaccgcg tggccgtgtt cgtcgaccgc tgtggccgtc atgagctcca gcgcgacgcc
      121 ttcgcgttct acaagcaaac ggtgaccgac ttcttcgcgc gttacgaatc cgaggaaatc
      181 ctcgggcgac ggtaacgtgt tgtcaggttc gggcggcgac gcaggttatc tacgcgttcg
      241 ccaagttcgc cgacggcgtt cgcgagcgaa aagaaaagcg aaacttgtcg gcacgcggca
      301 atcgcgttga gtcgctgcgc ccccacgtcg actcatcgcc atgattcttt ttcgccttct
      361 cgttctgctc tgcatcacgt ccgccgttca ggccgcatca tcgcccacgg ccgatctctc
      421 ttcgtggtcc gtcgaacaac aacccggggg gaccgtcacc gtggacggcg acgcgctcgt
      481 catcgaagac gccggtggct gcactgtctg gtttcgccaa aagctcaccg cgccagttga
      541 gatcagttac gaggtcacgg ccatttctcg cggcgggccg cacgatcggg tctccgacgt
      601 gaattgcttc tggatggcga gcgacccgcg ttcgcctgaa accctgccgg aaggccgctc
      661 aggtaaattc gcggactacg attcgctgcg cacctattac gtcggcatgg gcggttggaa
      721 caacaccaag acccgctttc gtcgctacgc cggcgcaggc gtgaaaccgt tgcttcccga
      781 acacgatctc agcgacgcgc agtttctcct cgtgccgaac aaaacttatc gcatcaagct
      841 cgtcgcccgc gacgggctcg ccgagttctg gcgcgatggc gaaaaaatct tctcctttcg
      901 cgaccccgaa ccgctcacgt ccggctggtt cggcgtgcgc accgtaaaga gccacctcga
      961 gatccggaat ctccggatca agtaagcggc gtcacccggc taactttcgc gtcgtcgcaa
     1021 caagccgctc tgccaggtct gtgaccgatt tcaatgtgcg cggttcgtta agttttcccg
     1081 ccgcatcgaa tgccttgttc gcatgcgcca ggatcacctg cccgggcacc acgtgacacc
     1141 caaggtgctg caacaactgc tgcgccatct tcaacgaacg cacaccgccg aacattcccg
     1201 gcgaagccga caccaccgcc gccacttttc cgataaacgg attgtcgtcc gcccgcgtgc
     1261 accaatcgat cgtgttcttc agcagcggcg tgatcatcga gttgtattcc ggcgaggcga
     1321 tcagcagcgc gggatgctct gaaatcaacg cgatcaattt cttcgcattc tcgggcagcc
     1381 cttccgcatc ctccaaatcg ccgtgataga tcggcagcgc gtaatcattg aggtcaacca
     1441 gcgtcacctc gccgcccgcc tccttcacct gctccaccgc gaccgcgagg aatttccgat
     1501 tgaacgattc acggcgagcg ctgccggaaa atgcaagaat gcgaacaagg gaggacatgc
     1561 gtcacggaat gcatctcacc tcgaacgaca aatccatcct gaacttgtcc actcgctggc
     1621 ccaccgcgct tgttcgccat cgtagggcct cgctacattc gcgcaaaatc cagaatgcgg
     1681 aaacgcttcc gatacgctcc gggagcaatg cccgtcgccc gtttgaaaag ccggcggaaa
     1741 aacgccggat cttcgtagcc gacttgccat cctatctcgt cgaccgaaga cgtcgtgcgc
     1801 tcgagccggc gtttcgcatc ctcgattcta agtcgctgca cgtaagcgag tggcgccaaa
     1861 cccgtcgcga ccgtaaaccg tcgcttgaac gtccgctccg cgagccgcga acgtttgatc
     1921 acctcgccca cgcaattagc gaccgaaaaa tgcgccgcca gccattgctg cgccgcctgg
     1981 atttcgccgt cgccgtgatc gatcctgccc tcgaacgcga tgtagggcgc gagtccgtcc
     2041 tgatgccatt gcagcgcgaa cgcccgggcg acgccttgcg ccgccgtcgc gccggcgtat
     2101 cgggcaatca aatacagcac caggtcgtgc cacgtcatcg acgcgcccga cgtcaccaac
     2161 tcttcacgcg tgccggagat caccagcacc cgctccggat gaatgcgcac cgccggatgc
     2221 accgccgcga actcgcgcgc gtagctaaaa tgca
//
LOCUS       DASPLT010001296         1061 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_302740, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001296 DASPLT010000000
VERSION     DASPLT010001296.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1061)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1061)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1061
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_302740"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..453)
                     /locus_tag="VEA63_13270"
     CDS             complement(<1..453)
                     /locus_tag="VEA63_13270"
                     /inference="COORDINATES: protein motif:HMM:NF014003.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent chlorinase/fluorinase"
                     /protein_id="HYD85026.1"
                     /translation="MRRVTQSAAEVGRGGACLQMILGADRGRAASIACKQAPAFCWRK
                     WMGSLGAVLVMTFAAGAEQALVLQTDFGTKDGAVAAMKGVAVGVNPRLAIFDLSHENT
                     PFDVWEAAYRLKQAAPFWPAGTVFVSVIDPGVGTPRKSVVLQTKSGHFF"
     gene            complement(450..>1061)
                     /locus_tag="VEA63_13275"
     CDS             complement(450..>1061)
                     /locus_tag="VEA63_13275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coproporphyrinogen III oxidase"
                     /protein_id="HYD85027.1"
                     /translation="PDHLSTYCLTFEEDTALWVKLSQGRVKLDVEHEARLYESTWAQL
                     AAAGYAQYEVSNFARPGHACRHNLNTWRMHEWVGLGPSGASQQAGWRGANIADLDQWL
                     VNVARGERVTEDRVAVTPALLAEDALIFGLRMNAGVDVTRWRERCPSAPWNAVEELLA
                     RLESDGLAVREKGNVRLTDRGRLVADSVGAEVMEAFTPAEAEV"
BASE COUNT          205 a          436 c          242 g          178 t
ORIGIN      
        1 aaaaaaatgc ccgctcttgg tttgcagcac gaccgacttt cgtggcgtgc cgacacccgg
       61 atcgatcacc gacacaaaca ccgtgcccgc cggccaaaac ggcgccgcct gcttcaaccg
      121 atacgccgcc tcccacacgt cgaacggcgt gttctcgtgg ctcaaatcga aaatcgccag
      181 ccgcggattc acgcccaccg cgacgccttt catcgccgct accgcgccgt ccttcgtgcc
      241 aaaatccgtc tgcagcacga gcgcctgctc cgcgcccgcc gcaaacgtca tcactaacac
      301 agcccccaaa ctccccatcc acttccgcca acaaaacgca ggagcctgct tgcaggcgat
      361 gctcgccgca cgaccgcgat cggcacccaa aatcatctgc aagcaggcgc ctccccgccc
      421 aacctccgcc gcgctttgcg tcactcgtct catacttcag cctccgccgg cgtaaatgcc
      481 tccatcactt ctgcacccac cgaatccgcc acgagccgcc cgcgatcggt caaccgcacg
      541 tttccttttt cgcgcaccgc gagcccatcg ctctcgagcc gcgccagcag ttcctccacc
      601 gcattccacg gcgcactcgg acaccgctcg cgccaccgcg taacgtccac gcctgcgttc
      661 atccgcagac caaagatcaa cgcatcctcc gccagtaacg ccggcgtcac cgccacgcga
      721 tcctccgtca cgcgttcgcc gcgcgccacg tttaccaacc actgatccag atccgcgatg
      781 ttcgcgccgc gccagcccgc ttgttgcgac gccccggacg gcccgagccc cacccattcg
      841 tgcattcgcc acgtattcaa attatgccga cacgcatgtc ccggccgcgc gaagttcgac
      901 acttcatact gcgcgtaacc cgccgccgcg agctgcgccc acgtcgattc gtagagccgc
      961 gcctcgtgtt ccacatcgag tttcacgcgc ccttgcgaaa gtttaaccca gagcgccgtg
     1021 tcctcctcga acgtcaggca atacgtcgac aaatgatccg g
//
LOCUS       DASPLT010001297         1505 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_39441, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001297 DASPLT010000000
VERSION     DASPLT010001297.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1505)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1505)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1505
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_39441"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(251..769)
                     /locus_tag="VEA63_13280"
     CDS             complement(251..769)
                     /locus_tag="VEA63_13280"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HYD85028.1"
                     /translation="MSVHIRSATPADVPLILSFIRALADYEKLTHEVEATEEKLHATL
                     FGERPAAECILAFADTVETANTRDAATAAAPIPAGFAIFFTNYSTFLAKPGLYLEDLF
                     VKPEFRGRGIGKALILHLARLANTRGCGRMEWTVLDWNAPAIAFYESLGAERKLAWQI
                     CRLTGPALEKFA"
     gene            complement(897..>1505)
                     /locus_tag="VEA63_13285"
     CDS             complement(897..>1505)
                     /locus_tag="VEA63_13285"
                     /inference="COORDINATES: protein motif:HMM:NF012652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HYD85029.1"
                     /translation="ITIEDPIEFVHPVKKSVIVHREVGEHTGSFAAALKGAMRHDPDI
                     LLLGEMREMETIKLALGCASMGMLVFGTLHTNNAPKTVDRIINAFPADEQNQVRVMLA
                     GCLAGVVAQLLCKRIPKGRCAVHEILLSHEALPNTIRSGQIANIKSIIESGSADGMVT
                     MDNSLMARIKDGTIEAKEAYMKAANKAVFAPLLKPGDLDAAH"
BASE COUNT          263 a          365 c          580 g          297 t
ORIGIN      
        1 cgacggagcc ggcgggaccg ttgcgatgga ggtcttcgtc ggaggaatat gaatacgccg
       61 cgccccaatc gacgagacga tccgcggtgg cggcaggttg cccaagcgag acaagggcgg
      121 ggagaacgag agcgagggga atgatcaggg agcggagacg acgcatggtc ggcagagtcc
      181 cttgaagtgg ctgggcgtca acgagtggaa ggacgtttcg acgagagccg gtgaatttac
      241 cgggccgacg tcacgcgaat ttctcaagcg caggaccggt gaggcgacag atttgccagg
      301 cgagttttcg ttcggcgccg agcgattcgt agaaggcgat cgcgggggcg ttccagtcga
      361 ggacggtcca ctccatgcgg ccgcagccgc gggtgttcgc gaggcgcgcg agatggagaa
      421 tgagcgcttt gccgatgccg cggccgcgga attccggttt cacgaaaagg tcttcgagat
      481 agaggccggg cttggcgaga aaggtggagt agttggtgaa gaagatcgcg aagccggcgg
      541 ggatgggtgc ggcggcggtg gcggcgtcgc gggtgttggc ggtttcgacg gtgtcggcga
      601 acgcgaggat gcactcggcg gcggggcgtt cgccgaacag cgtggcgtgt agtttttcct
      661 cggtggcttc gacctcgtgc gtgagtttct cgtaatcggc gagggcgcgg atgaaggaga
      721 gaataagcgg aacgtcggcg ggcgtggcgg aacggatgtg gacggacatg gaaggctgca
      781 tcgttctcgt tctctttctc gtaatcgttc tcgggttctg ctcgggctcg ggtcgggggt
      841 ttgcggcgcg gccgggagag aacgagaaag agaacgaaga acgagaacga ttcgaatcag
      901 tgcgcggcgt cgaggtcgcc gggttttagc agcggcgcga agacggcttt gttcgcggct
      961 ttcatgtagg cttccttggc ttcgatggtg ccgtccttga tgcgcgccat gaggctgttg
     1021 tccatcgtga ccatgccgtc ggcgctgccg ctttcgatga tggatttgat gttggcgatc
     1081 tgaccgctgc ggatcgtgtt cggcagggct tcgtggctta ggagaatctc gtggacggcg
     1141 cagcggcctt tgggaatgcg cttgcagagg agctgcgcga cgacgcccgc gagacagccg
     1201 gcgagcatga cgcggacttg gttttgttcg tcggcgggaa acgcgttgat gatgcggtcg
     1261 acggttttcg gggcgttgtt ggtgtggagc gtgccgaaga cgagcatgcc catcgacgcg
     1321 cagccaaggg cgagcttgat ggtttccatt tcgcgcattt cgccgagcag gaggatgtcg
     1381 ggatcgtggc gcatcgcgcc cttgagcgcg gcggcgaaag agccggtgtg ctcgccgact
     1441 tcgcggtgga caatcacgga tttcttcacg gggtgcacaa actcgatcgg gtcttcgatc
     1501 gtgat
//
LOCUS       DASPLT010001298         1036 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_96592, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001298 DASPLT010000000
VERSION     DASPLT010001298.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1036)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1036)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1036
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_96592"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..189)
                     /locus_tag="VEA63_13290"
     CDS             complement(<1..189)
                     /locus_tag="VEA63_13290"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373612.1"
                     /note="catalyzes the formation of
                     pyridine-2,3-dicarboxylate and 5-phospho-alpha-D-ribose
                     1-diphosphate from nictinate D-ribonucleotide; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide diphosphorylase
                     (carboxylating)"
                     /protein_id="HYD85030.1"
                     /translation="MLTHRTEHLIDLALDEDAGLGDVTSRAIFPASHRSRGFISAEQD
                     LVLCGVDVATRVFERVDGA"
     gene            complement(238..>1036)
                     /locus_tag="VEA63_13295"
     CDS             complement(238..>1036)
                     /locus_tag="VEA63_13295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HYD85031.1"
                     /translation="DMARQAKAKVIVFAGVVFMAETAKILSPDALVVVPDRESGCSLA
                     DSIDGDGVRRLKTLYPDATVVCYINSTADVKAESDVCVTSGNVYDIVANLPAKRVLFV
                     PDRLMAENVRAELKRRGVDKEIVSSDGTCMVHDQFTPPQIAEARAQFPGLKVVAHPEC
                     TAEIAALADFVGSTSEMMKYVKTTPAPYFLMLTECGLVSRLQVEDPEKNFIGGCRLCP
                     YMKMNTLEKVRDALKAPRPEQIITLDEDLRCRALRCIERMFELAPKK"
BASE COUNT          196 a          340 c          292 g          208 t
ORIGIN      
        1 cgctccatcc acgcgctcga acactcgcgt cgctacgtcc acgccgcaga gcacgaggtc
       61 ctgctccgcg gaaataaaac cgcgcgaacg atgcgaagcg ggaaagatcg ctcggctcgt
      121 gacgtcgcca agtccggcat cttcgtcgag tgccagatcg atcaggtgtt cagtgcggtg
      181 agtgagcatg attcagggtc cgggcgtttg tcatataata gatgacaaac cgttgggtta
      241 ttttttcggg gcgagttcga acatccgctc gatgcaccgc agtgcgcggc agcggaggtc
      301 ttcgtcgagc gtgatgattt gctcggggcg cggcgccttc agcgcgtcgc gcactttctc
      361 aagcgtgttc attttcatgt aaggacacag ccggcagccg ccgatgaagt tcttctccgg
      421 atcttccacc tgcagccggc tcaccagtcc gcattccgtc agcatcagga aatatggcgc
      481 gggtgtcgtc ttcacatact tcatcatctc gctcgtgctt ccgacgaagt ccgccaacgc
      541 cgcgatctcc gccgtgcact ccggatgcgc gacgactttc aatccgggga actgcgcgcg
      601 cgcctcggcg atctgcggtg gcgtgaactg atcgtgcacc atgcaggtgc cgtcggagga
      661 aacgatttcc ttgtcgacgc cgcgccgctt caactccgcg cgcacgttct ccgccatcaa
      721 acgatccggc acgaacaaca cgcgcttcgc gggaagattt gccacgatgt cgtaaacgtt
      781 tcccgacgtc acgcacacgt ccgactccgc tttcacgtcc gccgtgctgt tgatgtagca
      841 aacgaccgtc gcatcgggat aaagcgtttt caaccgccgc acgccgtcgc catcgatcga
      901 gtcggccagc gagcagcccg actcccgatc cggcacgacc acaagcgcat cgggcgagag
      961 gattttcgcc gtctccgcca tgaacaccac gccggcgaac acgatgacct ttgccttcgc
     1021 ctgacgcgcc atgtcg
//
LOCUS       DASPLT010001299         1021 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_281364, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001299 DASPLT010000000
VERSION     DASPLT010001299.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1021)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1021)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1021
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_281364"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..774
                     /locus_tag="VEA63_13300"
     CDS             <1..774
                     /locus_tag="VEA63_13300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HYD85032.1"
                     /translation="AALSASGLVMQAYFRNSLASPGLLGVSSGGAAGAVIAIGIGWSS
                     VSLFVLPLFAIVGSFAATGAVLILARRDTGTERLLLAGVALNALLGAVTSYVLTNFTL
                     TYERNAQMIFWLLGGLEDRTWEHVAMAAPVLLAAALLWPLGRSMDLLSLGVPEAQSLG
                     VDVRLLRRRLLVLSTVMTALATAVAGTIAFVGLVVPHVLRLMVGPEHRRLVPLSLIGG
                     AAFVIACDLAGRAGGGLRVGIVTALVGGPFFLWLLRRQP"
     gene            771..>1021
                     /locus_tag="VEA63_13305"
     CDS             771..>1021
                     /locus_tag="VEA63_13305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HYD85033.1"
                     /translation="MSALQIEHATVPGRIHDVSLRLERGMLVGLVGPNGSGKSTLLQL
                     AAGVLPGEGRVNWGGRAVADIPIVERGRCAAWVPQEAHF"
BASE COUNT          126 a          238 c          433 g          224 t
ORIGIN      
        1 gcggcgctgt cggcgagcgg gctcgtgatg caggcgtatt ttaggaacag ccttgcgagt
       61 ccgggattgt tgggtgtgag cagcggcgga gcagcgggcg cggtgattgc gatcggaatc
      121 gggtggtcgt cggtgtcgct gttcgtgttg ccgctgtttg cgatcgtggg atcgtttgcg
      181 gcgacggggg cggtgttgat cttggcgcga cgcgacacgg ggacggagcg gctgttgctc
      241 gcgggggtgg ccttgaacgc gttgctcggc gcggtgacga gctacgtgct cacgaacttc
      301 acgctcactt acgagcggaa cgcgcaaatg attttctggc tgctcggcgg gttggaggat
      361 cgcacgtggg agcacgtcgc gatggcggcg ccggtgttgc tggcggcggc gttgctgtgg
      421 ccattggggc ggtcgatgga tttgttgagc ctcggcgtgc cggaggcgca gagcttgggt
      481 gtcgacgtgc gtttgctgcg gcggcggttg ttggtgttgt cgacggtgat gacggcgctc
      541 gcgacagcgg tggcggggac gatcgcgttt gtcggtttgg tggtgccgca tgtgttgcga
      601 ttaatggtcg ggccggagca tcggagactc gtgccgttgt cgttgatcgg cggcgcggcg
      661 ttcgtgatcg cgtgcgattt ggcggggcgc gcgggcggcg gattgcgagt aggcatcgtg
      721 acggcgctgg tgggcgggcc gttttttctt tggttgttga ggcgtcaacc atgagtgccc
      781 tgcagatcga acatgcgacg gtgccggggc ggattcacga cgtgagtctg cggctcgagc
      841 gaggaatgct ggtcggtttg gtcgggccga acggctcggg aaaatcgacg ttgttgcaac
      901 tcgcggcagg cgtgctgcca ggtgaaggac gcgtgaattg gggcgggcgc gcggtggcgg
      961 atatccccat cgtggaacgc gggcggtgtg cggcgtgggt gccgcaggag gcgcatttcg
     1021 a
//
LOCUS       DASPLT010001300         1394 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_281378, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001300 DASPLT010000000
VERSION     DASPLT010001300.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1394)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1394)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1394
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_281378"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..816
                     /locus_tag="VEA63_13310"
     CDS             <1..816
                     /locus_tag="VEA63_13310"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HYD85034.1"
                     /translation="VALVFFAVVTLIPLVWMATSAVKNQTDFFTSFFLPHGDGFLGIA
                     WDRLTFANFSRLFAEHPIGRALFNSFFFASVSSAGATLACAMGGYALAKFQFPARPFL
                     TALVLGALIIPAPLLIAPSYQLLWQLGLLNAYSGLILPALAPAFGVFLFRQSMLNSVP
                     AELLESARMDGAGEFRIFFTIVIPLVRPMIGAFLLITFLGAWNNFIGPQIILQEPALF
                     PLSVTVNNLRGIYATDYGLIMSGTLISIAPVMCLFLLLQKEFISGLTTGAVKG"
     gene            complement(1153..>1394)
                     /locus_tag="VEA63_13315"
     CDS             complement(1153..>1394)
                     /locus_tag="VEA63_13315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD85035.1"
                     /translation="EELSPSRHVVQTSNRRFRDDEFFVSRALTSGHAKIRVRVKFTPV
                     EIPLMPGRPLPELAWSEIRYDAYSVMKPPRSAEGR"
BASE COUNT          228 a          532 c          306 g          328 t
ORIGIN      
        1 gtcgcgctcg tcttcttcgc cgtcgtcacg ctcatcccgc tcgtctggat ggcgacctcc
       61 gccgtcaaaa accaaaccga tttcttcacg tccttcttcc tccctcacgg cgacggtttt
      121 ctcggcattg catgggatcg cctcacgttc gcgaacttct cccgcctctt cgccgaacac
      181 cccatcggcc gcgcgctctt caactccttc ttcttcgcct ccgtttccag cgccggcgcc
      241 accctcgcgt gtgccatggg cggatatgcc ctcgccaaat tccaattccc cgcccgccct
      301 tttctcaccg cactcgtcct cggcgcgctc atcattcccg cgccgctgct catcgcgccc
      361 agctatcaac tcctctggca actcgggctc ctcaacgcct acagcggcct gattctcccc
      421 gccctcgcgc ccgctttcgg cgttttcctc ttccgccaat cgatgctcaa ctccgtcccc
      481 gccgaactcc tcgagtccgc gcgcatggac ggcgccggcg aatttcgaat cttcttcacg
      541 atcgtgattc ccctcgtgcg cccgatgatc ggcgcgtttc tcctcatcac ctttctcggc
      601 gcctggaaca acttcatcgg cccgcaaatc atcctgcagg aacccgcact cttcccgctc
      661 tcggtcaccg tcaacaatct ccgaggcatc tacgcgaccg actacggcct catcatgtcc
      721 ggcacgctca tttcgatcgc ccctgtgatg tgcctcttcc tcctcctcca aaaagaattc
      781 atctccggcc taaccaccgg cgccgtgaaa ggctgatttt tttaaccacg aatggacacg
      841 aattcacacg aatagtcaga ccggattgct cgcttgttat tcgtgtctct tcgtgtccat
      901 tcgtggttca atcccgccga tcgatccgtc tccatccgtg ttcatccgtg gttaaaaaaa
      961 tagccggagg gtttccaaag gttcacgcat cacgcctcaa gctcccacgg tccgccattc
     1021 gtgtgaattc gtgttcattc gtggttaaaa aatcccgcct ccctcgtctt ggtgattgaa
     1081 gcttaaagcg tctctggagc ttggtgcttg gatcttggag cttccgcgcc gcgccttccc
     1141 cgccgcgcaa cgctaccttc cctccgcgct gcgcggcggc ttcatcaccg aatacgcatc
     1201 ataccggatc tcgctccacg ccagctccgg cagcggccgc cctggcatca acggaatctc
     1261 caccggcgtg aatttcactc gcacgcggat cttcgcgtgc ccgctcgtca gcgcccgcga
     1321 cacgaagaac tcatcatcgc gaaaccgccg gttcgacgtc tgcaccacat gccgcgacgg
     1381 cgacaactcc tccg
//
LOCUS       DASPLT010001301         3465 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_158435, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001301 DASPLT010000000
VERSION     DASPLT010001301.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 3465)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3465)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3465
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_158435"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1158)
                     /locus_tag="VEA63_13320"
     CDS             complement(<1..1158)
                     /locus_tag="VEA63_13320"
                     /inference="COORDINATES: protein motif:HMM:NF024977.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HYD85036.1"
                     /translation="MTKTYRLEVDPRILELLGPNLYTNIYYVLAELIANAYDADAKNV
                     YIIAGKDDIRVEDDGHGMSYKAGDITKYLNVAGVSRTTEKDSFTRSKTRRKMGRKGVG
                     KLAALSVSENVDVLTIAKGEKSGFVLTRHPENGHELKAIPDEKIVFERIKNHGSAIIM
                     RHPQYRLHKTLAAVKRNLLKIFPLVDSNFRIHVIRGKESETIDDFDRSVMGELCALIT
                     LGDSFASLGDLVPDPYPKHRKDLVSAEEAKVLPVVMRANDGKEYEYSLEVLGWIGTYK
                     TTRGRKAELTDFPDNFLSLFANEKMGEFNILPVVGQNKLNEVYVVGQLHVDLFELTEL
                     PDMALSNRQGYKSDDPRYEAVREYVRKELLADILKRRETYTDLVNAKKKEVK"
     gene            complement(1155..>3465)
                     /locus_tag="VEA63_13325"
     CDS             complement(1155..>3465)
                     /locus_tag="VEA63_13325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD85037.1"
                     /translation="AAGRVVDLTGQVHEASTTSERMPPGIEERAENARRIVAERCLYG
                     VDLNPLAVELAKLSLWLVTLSKGRPFGFLDHNLRRGDSLLGVSRLEQLTQLSLDLSAT
                     RQGRLFGKAIERAVAEAVELRRKLREIPIRDIRDVEAMATLDADARKKLKAAELLADA
                     FIGAVFAAEGAGIETRLAAVAADAERVVKGEEGATDTLVKRAAKDLAKDSPSGDPRRP
                     FHWPLEFPDVFHGASPGFDALVGNPPFLGGKRITGVTGDAYRKWVVTHIAEGRRGSAD
                     LVAYFFLRAWSLLRDGGGFGLLAVNTIAEGDTRQVGLEAMVSAGATIHAAYPNEPWPG
                     SAAVVTSRVHVRKGNWNGVCSLAGREVPFISAYLSDQKEWSPKRLKANERTAFQGSVV
                     VGMGFVLQADDAKRMLDSDAKNADVIFPYLNGEDLNSHPEQRPSRWVISFWDWPEERA
                     REYRKPWAWIEERVRPERQRRDDNGKYVLRKPLPDRWWHYGDKRPGLYHAIGRGACFM
                     QHPKGWDVARAPHEEVLVAALVSKYFNPSLVQNDAIFNHKCAVFSTEKTAAMAALLNS
                     SLAQAWVWQQSSRLGMGLNFSPSDAIETLPFPQPNVLASFGKQGREYLQARRKVMADA
                     ANPIGLTKLYNRFHDATEVDPRIVRLRDMHREIDAAVIRAYGWDDLDLGHGYHEQPNL
                     AENDRVRFTISGAARAEVLRRFAELNRRRYEEEQASAAAKPSTTKARTKATPAEQGAL
                     ALDARAPKAKTSKTKAAAPAKKSSKRSSR"
BASE COUNT          585 a         1098 c         1069 g          713 t
ORIGIN      
        1 cttcacctcc ttcttcttgg cgttgacgag atccgtgtac gtctctcgcc gcttgagaat
       61 gtcggcgagc aattccttcc ttacgtactc gcgcacagcc tcgtaacgcg gatcgtcgga
      121 cttgtatccc tggcgattac tcagcgccat gtccggcagt tcggtcaact cgaacaaatc
      181 gacgtgcagc tggccgacga cgtagacttc gttcaactta ttctgtccga cgacgggaag
      241 gatgttgaac tcgcccatct tttcgttagc gaacaacgag agaaaattgt ccgggaagtc
      301 ggtcagctcg gctttgcgtc cacgcgtggt tttgtaagtt ccgatccacc ccagaacttc
      361 gagagagtac tcgtactctt taccgtcgtt cgccctcatg acgacgggga gaaccttcgc
      421 ctcctccgcc gagacgagat cttttcggtg tttcggatag ggatcaggaa cgaggtcgcc
      481 caacgacgca aagctgtcgc ccagcgtgat cagcgcgcac agctcgccca tgacgctgcg
      541 gtcgaagtca tcgatcgtct cgctttcctt gccgcgaatg acgtggattc gaaagttcga
      601 gtcgaccagc gggaagatct tgagaagatt ccgcttcacg gcggccaagg tcttgtggag
      661 ccggtactgc gggtggcgca tgatgatggc ggagccgtga ttcttgatgc gttcgaagac
      721 gatcttctcg tcggggatcg ccttcagctc atgcccattc tccggatgac gagtcagcac
      781 gaagccggac ttttcgcctt tagcgatggt caggacatcc acgttctcgg acacggaaag
      841 cgccgccagc ttaccgacgc ccttgcggcc catcttccgc cgcgtcttgg atctcgtgaa
      901 cgagtccttc tcggtcgtcc tcgatacgcc agccacattc aggtacttgg tgatgtcgcc
      961 tgccttgtag gacatgccat ggccgtcgtc ttcgacgcgg atgtcgtcct tgccagcgat
     1021 gatgtagacg ttcttcgcgt cggcgtcata cgcgttggcg attagttccg ccaaaacgta
     1081 gtagatgttc gtgtagaggt tgggccccag gagttcgagg atgcgtggat cgacttccag
     1141 ccggtacgtc ttggtcatcg agagctccgt ttgctggact tcttcgccgg ggcggcggcc
     1201 ttcgtcttcg aagtctttgc cttgggcgca cgcgcatcga gagcaagcgc gccctgctcg
     1261 gccggcgttg ccttcgtgcg ggctttagtt gtgctgggct tcgcggccgc cgatgcctgc
     1321 tcctcctcgt atcgccggcg gttcagctcc gcgaagcggc gaagcacctc agcgcgcgcg
     1381 gccccggaga tcgtgaaccg aacgcggtca ttctccgcga ggttcggctg ctcgtggtag
     1441 ccgtgcccaa ggtcgaggtc gtcccacccg taggcgcgta tgacggcagc gtcgatctcg
     1501 cggtgcatgt cgcgcaggcg tacgatgcgc ggatcgacct ccgtggcatc gtggaagcgg
     1561 ttgtagagct tcgtgaggcc gatcgggttc gccgcgtctg ccatcacctt gcggcgcgct
     1621 tgcagatact cgcgcccctg ctttccgaac gaggcgagca cgttcggctg cggaaacggc
     1681 aacgtctcga tcgcatcaga cggcgagaaa ttgagtccca tgccaagtcg cgatgactgt
     1741 tgccacaccc acgcctgagc aagcgaggag ttcagcaatg ctgccatcgc agctgtcttc
     1801 tcggtcgaaa agacggcaca tttgtgattg aagatcgcgt cattctgaac caaagaaggg
     1861 ttgaaatact tactaacgag cgcggcgacc aatacctctt catgaggcgc tcgagctacg
     1921 tcccagccct tggggtgctg catgaagcac gctcctcgtc cgatcgcgtg atacaaccca
     1981 ggccgcttat ctccgtaatg ccaccagcgg tcggggagcg gcttcctgag aacgtacttg
     2041 ccgttgtcgt cgcggcgctg ccgctccggc cggacccttt cttcgatcca cgcccagggc
     2101 ttcctgtact cgcgggctcg ttcctccggc caatcccaga agctgatcac ccaacgacta
     2161 ggacgctgct ccgggtgtga gttgagatct tcgccgttca agtacgggaa gatcacgtcc
     2221 gcgtttttgg cgtcactgtc gagcatgcgc ttcgcgtcgt ccgcctggag aacgaagccc
     2281 attccgacga cgaccgaccc ctgaaaagcc gtgcgctcat tggccttcag tcgcttggga
     2341 ctccattcct tttggtcgga gaggtacgcg gagatgaacg gaacctctcg cccagcgagc
     2401 gagcacactc cgttccaatt gcccttgcgc acgtgaacac gactcgtgac gactgccgcg
     2461 ctgccaggcc acggctcgtt cgggtacgcg gcatggatgg tcgctccggc gctgaccatc
     2521 gcttcgaggc ccacctgccg cgtgtcgccc tcggcgatcg tgttgaccgc gaggagcccg
     2581 aaaccgccgc cgtcgcgcag taggctccac gcacgcagga agaaataggc gacgaggtct
     2641 gccgagccac gacggccctc tgcgatgtga gtcactaccc attttcggta ggcgtcaccc
     2701 gtcacaccgg tgatgcgttt gccgccaagg aacggcgggt tgccgacgag ggcgtcgaag
     2761 cccggcgatg cgccgtggaa gacgtcgggg aactcgagcg gccagtggaa cgggcgacga
     2821 gggtcgccgc tcggcgagtc cttcgcgagg tccttggcgg cacgcttcac cagcgtgtcg
     2881 gtcgcgccct cctcgccctt cacaactcgt tcggcgtcag ctgcgacagc ggcgaggcgc
     2941 gtctcgatac ctgcgccctc ggcagcgaac accgcgccga tgaacgcgtc cgccagcagc
     3001 tcggcggctt tgagcttctt gcgcgcatcc gcgtcgagcg tggccatcgc ctcgacgtcg
     3061 cggatgtcgc ggatagggat ctcgcgcagc ttgcgccgta gttcgacggc ctcggctacg
     3121 gctcgctcga tggccttccc gaacagccgc ccctgccgcg ttgccgacag atcgagcgag
     3181 agctgcgtga gctgctcgag gcggctgacg ccgagcaggc tgtcgccgcg ccggaggttg
     3241 tggtctagaa agccgaacgg gcgacctttc gagagcgtca cgagccacaa cgaaagcttt
     3301 gccagctcga cggcgagtgg gttcaggtcg acgccgtaca ggcagcgctc ggcaacgatg
     3361 cggcgcgcgt tctcggcgcg ctcctcgatg cccggcggca ttctctcgct ggtggtggac
     3421 gcctcgtgga cttgcccggt gaggtcgacg actcggcccg ctgcc
//
LOCUS       DASPLT010001302         2049 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_117144, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001302 DASPLT010000000
VERSION     DASPLT010001302.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 2049)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2049)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2049
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_117144"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..622)
                     /locus_tag="VEA63_13330"
     CDS             complement(<1..622)
                     /locus_tag="VEA63_13330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HYD85038.1"
                     /translation="MVGAAAATMDDSLKPIPPEVIAEWWQPFADFLAQERRYSAYTLR
                     NYRQAFEDFYRWLVGARLWERGFDALDTRELRDFVIEAQRRFGRRTLHNHVSGLRSFF
                     KFWLRRGRVRRNPFLGVPLPKLEKRLPKFLTEEQVVRLLAGPQRLLENESIDAFTAHR
                     DQLAMELLYGAGLRVSELTGLNYGAIDLDAGVARVLGKGKKERLCPL"
     gene            complement(627..1313)
                     /locus_tag="VEA63_13335"
     CDS             complement(627..1313)
                     /locus_tag="VEA63_13335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG family protein"
                     /protein_id="HYD85039.1"
                     /translation="MSAMDELRETMRRLRSRDGGCPWDQEQTHASLVRCLIDEVSELI
                     DTIDREDYPHMREELGDVLIQVVFHACIAEEKGQFDLEDVAREINEKLIRRHPHVFGT
                     GKLDTSEQVITQWEAIKATEKKNGPPASGAGVFKELPPRLPALMFAEAIWKQIEKKQL
                     PVDGVVDRARVAAMSETLDEVELGQKLFELTAAARAKGLDPEGALRLHATKVMRAVEK
                     TAGDVPTVKA"
     gene            1419..>2049
                     /locus_tag="VEA63_13340"
     CDS             1419..>2049
                     /locus_tag="VEA63_13340"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HYD85040.1"
                     /translation="MSDKLTEIMAHKRREIAPLLRPISETELSRLNRSLPPPPSFAAA
                     LRRADGTLAIIAEIKRRSPSAGDIKAGVSSLEQARVYQSADVDALSILTDEKYFGGTL
                     DDLRGVTAHFRSTPNPRPCLRKDFMVHPLQVAEAREAGGSAILIIVRALDDEEIKSLH
                     EAAQAAGLDALFEIHREDELDRALAHGAKIIGVNNRDLAIFKTDLSLSER"
BASE COUNT          376 a          754 c          527 g          392 t
ORIGIN      
        1 cgagcggaca aagccgctcc ttcttccctt tgcccagcac gcgcgccacg cccgcatcca
       61 aatcgatcgc gccgtaattc agtccggtca gctcgctcac gcgcaatccg gcgccgtaaa
      121 gcagctccat cgcgagctga tcgcgatgcg cggtgaacgc gtcgatgctt tcgttctcga
      181 gcaaccgctg cggtccggcg agcaagcgca cgacttgctc ctcggtgaga aatttcggca
      241 gtcgtttctc cagcttcggc agcggcacac cgagaaaggg atttcgccgc acgcgtccgc
      301 gccgcagcca gaacttgaag aacgaccgca gccccgagac gtgattgtgc aatgttcgcc
      361 gtccgaaccg ccgctgcgct tcgattacga aatcgcgcag ctcgcgggta tcgagcgcat
      421 cgaagccgcg ctcccacaat cgcgcgccaa ccagccagcg ataaaaatcc tcaaacgcct
      481 gccggtaatt tcgcagcgtg taggccgagt aacgtcgctc ctgcgccaga aaatccgcaa
      541 acggttgcca ccattcggcg atgacctcgg gcgggatcgg tttcagcgaa tcgtccatcg
      601 tagcggcggc tgccccgacc accacctcac gctttcaccg tcgggacgtc gcccgctgtc
      661 ttctccaccg cgcgcatcac tttcgtcgcg tgcaatcgca acgcgccctc cggatcgagt
      721 cccttggcgc gcgccgcggc cgtcagctcg aacaatttct gccccagttc cacttcgtcc
      781 agcgtttcgc tcatcgccgc cacccgcgcg cgatcgacca cgccgtcgac cggcagttgt
      841 ttcttctcga tctgcttcca gatcgcttcc gcaaacatca gcgccggcag ccgtggcggc
      901 agctccttga acacgcccgc tccactcgcc ggcggaccgt ttttcttttc cgtcgccttg
      961 atcgcctccc attgcgtgat cacctgctcc gacgtatcca gttttccagt gccaaacaca
     1021 tgcggatgcc gccggatcaa cttctcgttg atctcgcgtg caacgtcttc gaggtcgaac
     1081 tggccttttt cctccgcgat gcacgcgtgg aacaccactt ggatcaacac gtcgcccagc
     1141 tcctcgcgca tgtgcggata atcctcgcga tcgatcgtgt cgatcagctc gctgacttca
     1201 tcgatcaggc agcgcacgag cgacgcatgc gtttgctcct ggtcccacgg acatccccca
     1261 tcgcgcgacc gcagccgccg catcgtctcc cttaattcgt ccatcgcgct cattgttcgg
     1321 atggccgcaa aaacgcgcag aaagcgcaag aactgaaccc cgcgtcgccg agattgtttc
     1381 ttgcgccttt tgcgcctctt tgcggctatc tcccttcaat gtccgacaaa ctcacggaga
     1441 tcatggcgca caagcgccgc gaaatcgccc cgctgctccg gccgatcagc gaaaccgaac
     1501 tctcccgcct caaccgctcg ctgccgccgc cgccgtcctt cgccgccgca ctgcgccgcg
     1561 ccgatggcac gctcgcgatc atcgccgaaa ttaaacgccg ctcgccttcc gccggcgaca
     1621 tcaaggccgg cgtatcctcg ctcgagcagg cgcgcgtcta tcaatccgcc gacgtcgacg
     1681 cgctttcgat cctcaccgac gaaaaatact tcggcggcac gctcgacgac cttcgcggcg
     1741 tcacggcgca ttttcgttcc acgccgaatc cgcgtccctg tttgcgaaag gatttcatgg
     1801 ttcacccgct gcaagtcgcc gaggcccgcg aagccggcgg cagtgcaatc ctcatcatcg
     1861 ttcgcgcgct cgacgacgag gaaatcaaga gtctccacga agccgcgcaa gccgccggcc
     1921 tcgacgcact tttcgagatt caccgggaag acgaacttga tcgcgcactc gcgcacggcg
     1981 cgaaaatcat cggcgtcaac aatcgcgacc tcgccatctt caaaaccgat ctctcgctca
     2041 gcgaacgcc
//
LOCUS       DASPLT010001303         1423 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_139270, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001303 DASPLT010000000
VERSION     DASPLT010001303.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1423)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1423)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1423
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_139270"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1423)
                     /locus_tag="VEA63_13345"
     CDS             complement(<1..>1423)
                     /locus_tag="VEA63_13345"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HYD85041.1"
                     /translation="AHASPNPPKVRASSRRVGGLRLFALGCVLLSSAYATAGNQHRSW
                     TNYGGGPDQSKYVVLDKIDKSNVAQLEVAWTYPTDDAIAYQFNPIVVDGVMFVFAKNN
                     SLVALDAATGKEIWIHANLRGLTRRGINYWESHDRSDRRLLFCLNNFLQAIDARTGKS
                     ILTFGQNGLVDLREGLGRDPKTVARVQSSSPGAIFENLILLGSSPGEGYVSPPGHLRA
                     YDVVTGERVWTFHTVPQPGEFGYDTWPRDAYKYAGGANAWGEITVDAERGIAYFPLGS
                     PTYDYYGADRIGSNLFGTSLLALDARTGKRLWHFQFVHHDLWDYDPTAAPQLITVRRD
                     GKTIDAVAQATKHGFMFVFDRVTGEPVWPIEERPVPPSDVPGEEAWPTQPFPAVLPPF
                     ARQSFTSDELTPIFLTPEERAEWKQRLDAGRTGLFTPPSLNYETISVPGAVGGANWGN
                     TAANPDAGIVYVLNQDFPSFYKL"
BASE COUNT          261 a          352 c          545 g          265 t
ORIGIN      
        1 tgaagtttat agaacgacgg gaaatcttgg ttgaggacgt agacaatgcc ggcgtcggga
       61 ttcgcggcgg tgttgcccca atttgcgccg ccgacggcgc cgggaacgga gatcgtttcg
      121 tagttcagcg aaggcggcgt gaagagcccg gtgcggccgg cgtcgaggcg ctgtttccat
      181 tcggcgcgtt cttccggcgt gagaaaaatc ggtgtgagtt cgtcggaggt gaacgactgg
      241 cgcgcgaatg gcgggaggac ggcgggaaac ggctgggtgg gccaggcttc ttcgccgggg
      301 acgtcacttg gcgggacggg gcgttcttcg atcggccaga cgggttcgcc ggtgacgcga
      361 tcgaagacga acatgaagcc gtgcttcgtg gcttgcgcga cggcgtcgat ggttttgccg
      421 tcgcggcgca cggtgatgag ttgaggcgcg gcggtgggat cgtagtccca gagatcgtgg
      481 tgaacgaact ggaaatgcca gaggcgtttg ccggtgcggg cgtcgagcgc gagcaggctg
      541 gtgccgaaga gattactccc gatgcggtcg gcgccgtagt aatcgtaggt cggcgagccg
      601 agaggaaaat aggcgatgcc gcgttcggca tcgacggtga tctcgcccca ggcgttggca
      661 ccaccggcgt atttataggc atcgcgcggc caggtgtcgt agccgaattc gccgggttgc
      721 ggaacggtgt gaaacgtcca cacgcgctcg cccgtgacga cgtcgtaagc gcgaaggtga
      781 ccgggcggcg aaacgtatcc ttctcctggg gacgagccga gaaggatgag gttttcgaag
      841 atggcgccgg gcgaactgga ttggacgcgg gcgacggttt tcggatcgcg gccgaggcct
      901 tcgcggagat cgacgagccc gttttggccg aaggtgagaa tggattttcc ggtgcgcgcg
      961 tcgatcgcct gaaggaaatt gttcaagcaa aacaacaggc ggcgatcgga gcggtcgtga
     1021 ctttcccagt agttgatgcc gcggcgggta agcccgcgaa gattggcgtg gatccagatt
     1081 tctttgccgg tcgcggcgtc gagggcgacg agggaattgt ttttcgcgaa gacaaacatc
     1141 acgccgtcga cgacgatcgg gttgaactga taagcgatcg cgtcgtcggt tggataagtc
     1201 cacgcgacct cgagctgggc gacgttggat ttgtcgattt tgtcgaggac gacgtatttg
     1261 gactgatcgg gaccgccccc gtagttggtc caggagcggt gctggttgcc ggcggtggcg
     1321 taggcggagg aaagcagaac gcagccgagg gcgaagaggc gcaagccgcc gacgcggcgc
     1381 gatgaggcgc ggactttcgg cgggttcggg gaagcgtggg cga
//
LOCUS       DASPLT010001304         1661 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_139298, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001304 DASPLT010000000
VERSION     DASPLT010001304.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1661)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1661)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1661
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_139298"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            879..>1661
                     /locus_tag="VEA63_13350"
     CDS             879..>1661
                     /locus_tag="VEA63_13350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 27 protein"
                     /protein_id="HYD85042.1"
                     /translation="MSLFNAILLSAAMLSPLPASDSDVSGTWRIDLPRSGGVSVRTYL
                     VLQPEGSELKGKVVINSAADLPLRKARWDGTEATFGIDWNASYRLRRDGEHLRVVIDY
                     NGKRTEEALATRVPESEVTPPSPLPLPAVTSLPDNGLARTPPMGWNSWNHFADRVDDR
                     IVREAADALVATGLAAAGYVYINIDDCWEGARDTGGNLVPNSKFPDMKALADYVHSRG
                     LKLGLYTSPGPVTCGGYVGSHGYEEQDARAFAAWGIDYLKYDW"
BASE COUNT          322 a          543 c          488 g          308 t
ORIGIN      
        1 gacagctcga ccacttcctc ttcggcgccc gtggtgctcg acggcgcggt ttgcgcgttc
       61 aagccgggac aaagaaagcc ggccacgcag ccggtcgcca aaagcaggcc gcgccggaag
      121 ccggcgacgg acgggcgcgc accgcgaaat cggcgatgcg gtaactccat cgaaacgcga
      181 gccgatgcac ggctctcact caaatgtcgg ttgttcatgt tgggttggga tgtcaggcaa
      241 atttccaccc gttcgctgcc gtcggcttcc gaggtgacca ggcgacggcg tcgtaatgcg
      301 acgcgcgaac gccaggacaa cgcggacgac ggcggtcctc gcgaaagaag aagtcgaaca
      361 gggaaggagc tgtcactggg ggttggtcgt tactcgatcc gctcgggaaa tccgggcggc
      421 cgttgaggag cagaacgcgg tcaacgtccg tgtgcctcgg ggcggcagtt ggaaaaccaa
      481 cgcgcttggg ggttgtcaac cggatttatc atacgataaa tttataatga acagcccata
      541 ggtgagaccc ggcgcttcat gggccccgta ttacgacggc tggaaaagcc ttcagaaccg
      601 cgcatgttca tcgcgtccac cggcttcaaa ccaagaccgt gtaatcatcg caatctacta
      661 ctatggagct gttaactgaa gatgacattg tccggatcac tacgtgcgtc gcagatgcca
      721 gccggcggcc tgcaggtgct cggtttttcc gcgctcggct cgattgccag ccgaccgtcg
      781 ccaccggcct gatttgtcat tgactaaata agcagcacgc cgacgctccg ccacgcttcg
      841 cttggccgac cgccttggcg tgcagagacc cgcttcccat gtcgctgttc aacgcgattc
      901 tcctttcagc agctatgctc tctcctcttc ccgcttccga ctctgacgtt tccggaacct
      961 ggcgcatcga tcttccccgc tcgggcggcg tcagcgtgcg cacttatttg gtgcttcagc
     1021 cggaaggctc ggaattgaag ggcaaagtcg tgatcaactc cgccgccgat cttccgctgc
     1081 ggaaagctcg ctgggacggc accgaggcca cgttcggcat cgactggaac gcctcgtatc
     1141 gactccgccg cgacggcgaa cacctccgcg tggtgatcga ttacaatggc aagcgcaccg
     1201 aagaggctct cgccacgcgc gttcccgaaa gcgaagtcac cccgccgtcg cccttgccct
     1261 tgcccgccgt gacgagcctc cccgacaacg gcctcgcccg caccccgccg atgggttgga
     1321 acagttggaa tcatttcgcc gatcgcgtcg acgaccgcat cgtccgcgaa gccgccgacg
     1381 cactcgtcgc caccggcctc gccgccgccg gctacgttta catcaacatc gacgattgct
     1441 gggaaggcgc gcgcgacacc gggggaaacc tcgttcccaa ttcgaagttt cccgacatga
     1501 aagcgctcgc cgactacgtt cacagtcgcg ggttgaaact cggcctctac acctctcccg
     1561 ggcccgtcac ctgcggcggt tacgtgggaa gccacggtta cgaggaacag gacgcacgag
     1621 ccttcgccgc ctggggcatc gactatttga aatacgactg g
//
LOCUS       DASPLT010001305         1500 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_139331, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001305 DASPLT010000000
VERSION     DASPLT010001305.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1500)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1500)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1500
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_139331"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..517
                     /locus_tag="VEA63_13355"
     CDS             <1..517
                     /locus_tag="VEA63_13355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD85043.1"
                     /translation="ISIIDTSPLMKRYSWEKLGSLRIAGTFNVGRNPVSMCFARRGDS
                     NLPLLPKKSNGTQSAPDPYNNLFYIAVRGDRKVVGVVTYQGKGAVYRTIKDKRMIDPV
                     AVSTAIRGPIVSVADFRGKKVVSFRVGAIYDARNKKTYGCGADGKAPFEFAGELAIKG
                     HPFLLNSTNVN"
     gene            824..>1500
                     /locus_tag="VEA63_13360"
     CDS             824..>1500
                     /locus_tag="VEA63_13360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD85044.1"
                     /translation="MISSSLVRFVLAGLAMALCLQSSVSASTWIPLTITRLTLVNSLT
                     NQDIRQISAEDTIDLSKVGSRITVRADVAGKVGSVAFLLNGKTSSVENTAPYSMHGDA
                     DGDFKRWAPKPGTYQLRVIPYPSRNRGGKAGAATEITLRVIEGTVTPPAPQPEPKPEP
                     EPEPEQPAPTSLSVTKLMLINAKTNKELRELAPGETVSLAQDGTELSVRAVVSGTAGS
                     VQFYLNG"
BASE COUNT          308 a          489 c          403 g          300 t
ORIGIN      
        1 gatcagcatc atcgacacgt cgccgctcat gaagcgctac tcgtgggaga agctcggttc
       61 attgcgcatc gccggcacgt tcaacgtcgg tcgcaatccg gtgagcatgt gtttcgcgcg
      121 tcgcggcgac agcaatcttc cgctgctacc gaaaaaatcc aacggaacgc agagcgcgcc
      181 cgatccctat aacaacttgt tctacatcgc cgtccgcggc gatcggaagg tcgtcggcgt
      241 cgtcacgtat caaggcaagg gcgccgtata tcgcacgatc aaggacaaac gaatgatcga
      301 tcccgtcgcc gtcagcaccg ccattcgcgg cccgatcgtt tccgtcgccg attttcgcgg
      361 caagaaggtc gtgagcttcc gcgtcggcgc gatttatgat gctcgcaata aaaagactta
      421 cggctgcggc gccgacggca aagcgccctt cgagttcgcc ggcgaactcg ccatcaaagg
      481 ccaccctttc ctgctcaatt ccacgaacgt aaactgatcg caggtccaac ctaaccatca
      541 agcgatagag cgcggcgtgc ggtcatcacg ccgcagaaaa tagaaccgac gatcctcacg
      601 ggtcgtcggt tttttcgttt cttctttcgc gttcggcgct tgcgcagtcg catcagtcgc
      661 ggtgcgtgcc acggtggaat ttacccgcac atgtatcggg gattctacta caggaagctt
      721 acgagttctt gactaggtgc aggcttccca tccgccccaa ggatgttctc ttatgcggcg
      781 tccggcatgt agccagtccg tcaatacaga aaacatcaca gtaatgatct cttcgtcctt
      841 agtccgtttc gttctcgccg gtcttgcgat ggccctttgc ctgcaatcgt ccgtttccgc
      901 ttccacttgg attccgctca ccatcacgag gctcacgctg gtgaattcgc tcaccaacca
      961 agacattcgc cagatctcgg cggaggacac gatcgatctg tccaaagtcg gttcgcgtat
     1021 caccgtccgg gccgatgtcg cgggcaaagt cgggagcgtc gcttttctgc tcaacggcaa
     1081 aaccagctcg gttgaaaaca ccgcacctta ctcgatgcac ggtgacgccg atggcgactt
     1141 caagcggtgg gcgccgaaac ccggcaccta ccagctgcga gtgattccct atccgtcgcg
     1201 caatcgcggc ggcaaagccg gggccgccac ggagatcacg cttcgtgtca tcgaaggcac
     1261 cgtcactccg cccgctcccc agcccgagcc caaacccgag ccggaacctg agcccgagca
     1321 gcccgccccg acctcgctca gcgtgacgaa gctcatgctg attaacgcga agaccaataa
     1381 agagcttcgc gagcttgctc ccggcgaaac cgtttcgctc gcgcaggacg gcaccgagct
     1441 cagcgttcgc gccgtggtca gcggcaccgc cggcagcgtc cagttttatc tgaacggcaa
//
LOCUS       DASPLT010001306         1028 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_281433, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001306 DASPLT010000000
VERSION     DASPLT010001306.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1028)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1028)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1028
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_281433"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..157)
                     /locus_tag="VEA63_13365"
     CDS             complement(<1..157)
                     /locus_tag="VEA63_13365"
                     /inference="COORDINATES: protein motif:HMM:NF013999.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein"
                     /protein_id="HYD85045.1"
                     /translation="MSPDQIKEHLKQVKYPGFSRDIVSFGLVRSAAIVEGTAKVSLAI
                     TTSDPKTP"
     gene            262..>1028
                     /locus_tag="VEA63_13370"
     CDS             262..>1028
                     /locus_tag="VEA63_13370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012377372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter Tol"
                     /protein_id="HYD85046.1"
                     /translation="MQKFLHLFLFLAACAAPACAQRDIGYVDVVAERNTLPVRVSANS
                     AELNQLALQAFGAHGRYRLTASGYAYDIKFSQVTPTQVRVDITRGSAATPVASQVATG
                     ANAREALLRAADIAVERTNGLGLKGFFTAQLAFIGERTGKKEVYTSDLFFGSAKQITR
                     DNAHALTPRWSPDGGKLLYTSFFKAGFPDIFQIDLGTYQRTTFVSFKGTNTGARFSPN
                     GQQVAMVLSGEGNAEVYVGTPQGRQIRRLTRTDGVEAS"
BASE COUNT          199 a          282 c          347 g          200 t
ORIGIN      
        1 gcggcgtctt cggatcgctg gtcgtgatcg cgagcgaaac tttcgcggtt ccctcgacga
       61 tggccgccga gcggacgagt ccgaaggaga cgatgtcacg gctgaacccc ggatatttga
      121 cctgcttgag atgctctttg atttggtcgg ggctcacggc gggaaattgt cggcggaaaa
      181 ggaaataaag ttgttatgcg cggatgcggt gtaaatagaa atgccgaaac cttcggcacg
      241 tttaagccac acagttacgt catgcaaaaa tttcttcatc tcttcctttt tctcgcggcg
      301 tgcgcggcac ctgcgtgcgc acagcgcgac atcggttatg tcgacgtcgt cgcggaaaga
      361 aacacgttgc cggtgcgcgt ttcggcgaac tcggcggagt tgaaccagct cgcgctgcag
      421 gcgtttggcg cgcacggccg gtatcggctg acggcgagcg gctacgctta cgacatcaag
      481 ttctcgcagg taacgccgac gcaggtgcgc gtggacatca cgcgcggcag cgcggcgacg
      541 ccggtggcct cgcaggtcgc gaccggcgcg aacgcgcgcg aggcgctgct gcgcgcggcg
      601 gacatcgcgg tggagcggac gaacggtctg ggattgaaag gatttttcac ggcgcagttg
      661 gcgttcatcg gcgagcgcac gggcaagaaa gaggtttaca cgtcggattt gtttttcggc
      721 agcgcgaagc agatcacgcg cgacaatgcg cacgcgctga caccgcgctg gtcgcccgat
      781 ggcggcaagc tgctctacac gagttttttc aaggcgggct ttccggatat ttttcaaatc
      841 gatctcggga cctatcaacg cacgaccttc gtgagtttca aaggcaccaa cacgggcgcg
      901 cgattcagtc cgaatgggca gcaggtcgcg atggtgttga gcggcgaggg caatgccgaa
      961 gtttacgtgg gcacgccgca aggccgtcag atccggcggc tgacgcggac ggatggcgtc
     1021 gaggcgtc
//
LOCUS       DASPLT010001307         1003 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_139333, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001307 DASPLT010000000
VERSION     DASPLT010001307.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1003)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1003)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1003
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_139333"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..210
                     /locus_tag="VEA63_13375"
     CDS             <1..210
                     /locus_tag="VEA63_13375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative dsRNA-binding protein"
                     /protein_id="HYD85047.1"
                     /translation="RVQPQHGNHALRYEVMQTEGEDHARIYEVAVFLNDRLLGQGRGP
                     SKKTAEEAAARAALETLKASSDDER"
     gene            824..>1003
                     /locus_tag="VEA63_13380"
     CDS             824..>1003
                     /locus_tag="VEA63_13380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD85048.1"
                     /translation="MADLATLHRVKVTGVCPPARGAIIAELTRTQPSPVWIVVTENLK
                     AAEELAEDLAFFHAAA"
BASE COUNT          210 a          340 c          258 g          195 t
ORIGIN      
        1 cgcgtccagc cgcagcacgg caatcacgcg ctccgctatg aagtgatgca gaccgaaggc
       61 gaagaccacg cacgcatcta cgaagtcgcg gtttttttaa acgaccgtct gctcggacaa
      121 ggccgcggcc catcgaaaaa aaccgccgaa gaagccgccg cccgcgccgc gctcgaaacg
      181 ctaaaagcct ccagcgacga cgaaaggtag gagcctgctt gcaggcgccc acgcctccaa
      241 aaatggagcc gcggcgcccc cgccgcacat agccggaata attcatttga acgacgaatg
      301 gacgcgaatt gacacgaatg aaaccgagtt cgaatgcgtc gtcttctcgg aacaccgaag
      361 ggtgatgtaa cgatcggcct gaaagctttc cttcggctcc tttgaattcg tgtccattca
      421 tgatttctct tctgtatgag tccggctttg acccagccta cgtgagtgcc ggcggcggtg
      481 ccgaaccgaa tcggagcccc attaattgaa caaaaggaaa cgaaggccac agcgagaaac
      541 ggctcttcgt tcccttcgtt gccttttgtt caaactccgt cgctagaaaa tgcacgccgg
      601 aaaacaatcc gttgaagcgt tgtgtcccag cgcccgacac gcggcctttg ccttgctccg
      661 cgacctctgt gtcaccgctc cgtgtcctct ctgtcccaaa gcctgacgtt tttccgtgtg
      721 aagatgtggc ttagcgcatg agcgccccag tgccacgagc acccgccgcg ccccgaaggt
      781 tgcgctccgc gccaaccgtc gtttcctgct tccctccctt ttcatggccg atctcgccac
      841 tctccatcgc gtcaaagtca ctggcgtttg cccacccgcc cgcggcgcaa tcatcgcgga
      901 gctgacgcgc acgcagcctt caccggtctg gatcgtcgtc accgaaaacc tcaaagccgc
      961 cgaggagctc gccgaggacc tcgccttttt tcacgccgcc gcc
//
LOCUS       DASPLT010001308         1217 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_54939, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001308 DASPLT010000000
VERSION     DASPLT010001308.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1217)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1217)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1217
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_54939"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            186..>1217
                     /locus_tag="VEA63_13385"
     CDS             186..>1217
                     /locus_tag="VEA63_13385"
                     /inference="COORDINATES: protein motif:HMM:NF013591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HYD85049.1"
                     /translation="MATSRVWLALTGATLLAACSDEPATNPAVTDNADPAAETAAVPV
                     ATENPFFEPSSLPLQYPPFDRIRDEHYLPAFERGMEEQLAEIAAITAQTEAPTFDNTI
                     LPLERSGRLLTRVANVFFALSSAHTNDAIQKLEVELSPRLSAHSDAILLDEALYARIS
                     DLYDRRDSLGLDPESLRLVEITHRDFVRAGAQLSPEDKDRLRAMNTELAELETTFSQN
                     VLAEVNASAIVVDSSEELAGLSDAEIKAAADEAVARDMPGKYVLPLLNTSGQPLLSTL
                     ENRDLRQRIMETSLARGSRGGEFDNTEILSRTARLRAERAQLLGYPNHAAYVLEEQTA
                     LTVDAVNQRL"
BASE COUNT          241 a          390 c          352 g          234 t
ORIGIN      
        1 cagctgcagc tcatcgctgg cgcgggcttc gttggcatcg gcatctggac gctgtcgcgc
       61 gcgctgatgg cggaagcttg accgttcgcg tattttcgtg ggaatgtgtt tcgtaatgaa
      121 tttcccggta tttgtgcagt atttcttcct ttcgacgttt cgctgtattt cctcaaggag
      181 atcccatggc tacttcgcgc gtatggctcg ccctgaccgg cgccacattg ctcgcggcct
      241 gttccgatga accggctacg aatcccgctg tcaccgacaa cgcagatcca gccgccgaga
      301 ccgccgccgt gccagttgcc actgaaaatc ccttcttcga accgagctca ttgccactgc
      361 aatacccgcc gttcgaccgg attcgcgacg aacattacct gcccgcgttc gagcgcggca
      421 tggaagaaca gctggccgag atcgcggcaa tcaccgcgca aaccgaagcg ccgacattcg
      481 acaacacgat cctgccgctc gaacgttccg gccgtttgct gacgcgcgtg gcaaatgtgt
      541 tctttgcgct gagttcggcg cacacgaacg atgctatcca aaaactcgaa gtcgaactgt
      601 cgccgcgcct gtctgcgcac agcgacgcga tccttctcga cgaagcgctg tatgcgcgca
      661 tcagcgatct gtatgaccgc cgcgactcgc tcgggctcga tcctgaatcg ctgcgtctcg
      721 tcgaaatcac gcatcgcgat ttcgtccgcg ccggcgcgca gctgtctccg gaggacaagg
      781 atcgcctgcg cgcgatgaac accgagctcg ccgaactgga aacgacgttc agccagaacg
      841 tactcgcgga agtgaacgcg agcgcgatcg tcgtcgacag cagtgaagaa ctcgcaggcc
      901 tcagtgatgc agaaataaag gcggcagccg acgaagccgt cgcacgcgac atgccgggca
      961 agtacgtgct accgctgctc aatacgagcg gtcagccctt gctgagcacg ctcgagaatc
     1021 gcgacctgcg ccagcgcatc atggaaactt cgctcgcgcg cggcagtcgt ggcggcgagt
     1081 tcgacaacac cgaaattctg agccgcaccg cgcggttgcg agccgaacgc gcgcagttgc
     1141 tcggttatcc gaatcacgcg gcgtatgtgc tcgaggagca gaccgcattg acagtcgatg
     1201 cggtgaacca gcgcctc
//
LOCUS       DASPLT010001309         1304 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_76165, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001309 DASPLT010000000
VERSION     DASPLT010001309.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1304)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1304)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1304
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_76165"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..471)
                     /locus_tag="VEA63_13390"
     CDS             complement(<1..471)
                     /locus_tag="VEA63_13390"
                     /inference="COORDINATES: protein motif:HMM:NF012356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HYD85050.1"
                     /translation="MAFHFPLMPRLFMSVRMEDRTPIIDILDQTPAIPETCQWAMFLR
                     NHDELTLEMVTEEERDYMYRMYAADARARINLGIRRRLAPLLNNDRRRIELLNALLLS
                     MPGTPVIYYGDEIGMGDNIYLGDRNGVRTPMHWSSDKNAGFSRANPQSLYLPIIL"
     gene            complement(450..1295)
                     /locus_tag="VEA63_13395"
     CDS             complement(450..1295)
                     /locus_tag="VEA63_13395"
                     /inference="COORDINATES: protein motif:HMM:NF012356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HYD85051.1"
                     /translation="MSFPEANPSPVAEPLWYRNAVIYQAHVRSFYDSDGNGIGDFRGV
                     TQKLDYLQDLGISALWLLPFYPSPLRDDGYDIADYYAINPIYGTLDDFKEFLEEAHRR
                     SLRVITELVINHTSDQHPWFQRARRAKPGSVERDYYVWSDTTEKYQDARIIFRDFEQS
                     NWAWDPVANSHYWHRFYSHQPDLNFDNPVVHEEIVRVLDFWLELGVDGLRLDAIPYLY
                     EREGTNCENLPETHAYLKTLRQHVDAKYGDRMLLAEANQWPEDAVKYFGEGRATSATW
                     RFIFR"
BASE COUNT          276 a          425 c          347 g          256 t
ORIGIN      
        1 aagaatgatc ggcagataaa gactctgcgg attcgcccgc gagaagcccg cattcttgtc
       61 cgagctccag tgcatcggcg tccgcacgcc attgcgatcg ccgagataaa tgttgtcccc
      121 catcccgatc tcgtcgccgt aataaatcac cggcgtcccc ggcatcgaga gcagcagcgc
      181 attcaataat tcgatccgcc gccgatcgtt gttcaacaac ggcgccagcc gccgccggat
      241 gcccagattg atccgggcac gcgcgtccgc cgcatacatc cgatacatgt aatcgcgctc
      301 ctcctccgtc accatctcga gcgtcagctc gtcgtgattg cgcaaaaaca tcgcccattg
      361 acacgtctct ggaatcgccg gcgtttgatc gaggatgtcg atgatcggcg tgcgatcctc
      421 cattcgcacc gacatgaaca gccgcggcat cagcggaaaa tgaaacgcca tgtggcactc
      481 gtcgcccgcc cttcgccgaa atatttcacc gcgtcctcag gccactgatt cgcttcggcg
      541 agcagcatgc ggtcgccgta cttcgcgtcg acgtgctggc gcagggtctt caggtaagcg
      601 tgcgtttccg gcaggttctc gcaattcgtg ccctcccgtt cgtaaagata cggaatcgcg
      661 tccagccgca gcccgtccac gccgagttcc agccagaaat ccagcacgcg cacgatctcc
      721 tcgtgcacca ccggattgtc gaaattcaaa tccggctgat gcgaataaaa gcgatgccag
      781 taatgcgagt tcgccaccgg atcccacgcc cagttcgact gctcgaagtc ccgaaaaatt
      841 atccgcgcat cctgatactt ctccgtcgtg tcgctccaca catagtaatc gcgctcgaca
      901 ctgcccggct tcgcgcggcg cgcgcgctga aaccacggat gctggtccga ggtgtgattg
      961 atcacgagct ccgtgatcac gcgcagcgac cgccgatgcg cttcttcgag gaactccttg
     1021 aaatcgtcca gcgtgccgta gatcgggttg atcgcgtaat agtccgcgat gtcgtagccg
     1081 tcgtcccgca acggcgacgg ataaaacggc agcagccaca acgccgagat gccgaggtcc
     1141 tgaaggtaat ccagcttctg cgtcacgccc cgaaagtcgc cgattccatt gccgtcgctg
     1201 tcgtagaacg agcgcacgtg cgcctgataa atcaccgcgt tgcgatacca tagcggctcg
     1261 gcaacgggcg acggattagc ttcaggaaac gacatggttt aaaa
//
LOCUS       DASPLT010001310         1184 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_281476, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001310 DASPLT010000000
VERSION     DASPLT010001310.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1184)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1184)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1184
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_281476"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..221)
                     /locus_tag="VEA63_13400"
     CDS             complement(<1..221)
                     /locus_tag="VEA63_13400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HYD85052.1"
                     /translation="MRNDSSLPTQHLKIVVIGGSGLIGSKLTTLLRQLGHEVNAASPS
                     SGVNTLTGEGLADALAGAQVVVDVANSPS"
     gene            296..>1184
                     /locus_tag="VEA63_13405"
     CDS             296..>1184
                     /locus_tag="VEA63_13405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009508830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HYD85053.1"
                     /translation="MSAFENLALLRAFVAIVECGSISAGARRLRVSQPGLSRQLRTLE
                     EICGTALLRRDTHRMSVTEAGRRLLDDAKSILTHAEEADRRLREGHTTLSGQLRLFAT
                     VDLGQFIVSRLVSQFLRDHPKVTATLALTNRPLHMIQEGCDVGILPGKITDESVIARP
                     AGTLALQLAASPLLSKSRPTPKEPEDLQSWPWVSLDGSQFWSARELTLHRDGAERTLR
                     VSPLLLSEGVTSVREAVREGLGVAVLPNWLIEDDLRSGTLVSVLPRWRPKDVMVHVVY
                     AGQRVLPARVGAFIDFAVSY"
BASE COUNT          198 a          340 c          419 g          227 t
ORIGIN      
        1 aacgagggcg agtttgcgac gtcgacgacg acctgcgcgc cagcgagcgc gtcggcgagg
       61 ccttcgccgg tgagcgtgtt gacgcctgaa gacggcgagg cggcgttgac ctcgtggccg
      121 agctgccgga ggagagtcgt gagtttggaa ccgatcaggc cggagccgcc gatcacgacg
      181 attttaagat gctgggtggg aagggaggag tcgtttctca tgacggctcg atgatgcacc
      241 cgattcgttt ttcggtgtag ccgtcgcgcg ccaataacag tcatgctgtt tccgcatgag
      301 cgctttcgaa aacctcgcgt tgctccgcgc attcgtggcg atcgtggaat gcggcagcat
      361 ctccgcgggc gcgcggcggc tgcgcgtgtc gcagccggga ctgagccggc agttgcgcac
      421 gctggaggaa atttgcggga cggcgctgct tcggcgcgac acgcatcgga tgagcgtcac
      481 cgaggcgggg cggcgcttgc tcgacgatgc gaaatcgatt ttgacgcacg ccgaggaagc
      541 ggaccgccgg ctgcgcgaag gtcacacgac gttgagcgga caactgcggc tctttgcgac
      601 ggtggatctc gggcagttta tcgtctcgcg gctggtcagt cagtttcttc gcgaccaccc
      661 gaaagtcacg gcgacgctcg cgctgacgaa tcgtccgctg cacatgattc aagaaggctg
      721 cgatgtgggg attctgccag gcaagatcac cgacgagagc gtgatcgcgc ggcctgcggg
      781 cacgctcgcg ctccaactcg ctgcgtcgcc gcttctgagc aaaagccgtc cgacgccgaa
      841 agaacccgag gacctccaat cgtggccgtg ggtgtcgctg gatggctctc aattttggag
      901 cgcgcgcgag ctcacgctgc accgcgacgg cgcggagcga accttgcgcg tttcaccgct
      961 gttgctttcg gaaggagtca cgagtgtgcg cgaggcggtg cgcgaagggc tcggagttgc
     1021 cgtgctgccg aactggctga tcgaggatga tctgcgctcg ggaacgctcg tatcggtttt
     1081 gccgcgatgg agaccgaagg acgtgatggt tcacgtggtc tacgccggac agcgtgtgct
     1141 gccggcccgc gtgggtgcgt tcatcgattt cgcggtttct tacc
//
LOCUS       DASPLT010001311         1267 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_76204, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001311 DASPLT010000000
VERSION     DASPLT010001311.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1267)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1267)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1267
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_76204"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1249
                     /gene="queG"
                     /locus_tag="VEA63_13410"
     CDS             <1..1249
                     /gene="queG"
                     /locus_tag="VEA63_13410"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009508526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HYD85054.1"
                     /translation="AGGGGFRAGATMSDVRQEELRRRLFALGFDEVRFAAIAGGDGSE
                     GEALREWLAAGMHGEMGWMERSAEKRGDPTLVLPGAQSMILLGVSYSADRLVTTEAVR
                     EKPVWARYALHEDYHDTMKPALVEAGRVLEELYGVTSGDYRYYVDTGPVLERSWAARA
                     GVGFVGKNAMLISRTHGNWLFLSAMLCRVPLQADEPVRKKFVDEDAGAIGLLCGKCTR
                     CIDACPTNAIVKPGVVDARKCISYQTIENKGVIPRELRAGIGRRIYGCDVCLEVCPWN
                     RFAREGRAMLLVARGDLAGMAGAEILALTPETFAERFRRTPIKRLKLAGLLRNACIVA
                     GNARDKSLLPQLVTLTRHALPLVRAHAVWAVFQIAGQTRATELLAEARAAETAGEVLA
                     EYAAEERERGDNGGIERPRPTGE"
BASE COUNT          215 a          283 c          537 g          232 t
ORIGIN      
        1 ggcgggaggc ggcggatttc gtgcgggcgc gacgatgagc gacgtgcggc aggaggagtt
       61 gcggcggcgg ttgttcgcgt tgggcttcga cgaggtgagg tttgcggcga tcgcgggcgg
      121 cgacgggagc gaaggcgaag cgttgcgcga gtggctcgcg gcgggaatgc acggcgagat
      181 ggggtggatg gagcgcagtg cggagaagcg aggcgatccg acgctcgtgc taccgggcgc
      241 gcaatcgatg attttgctcg gcgtgagtta ttcggcggat cggctggtga cgacggaagc
      301 ggtgcgcgag aaaccggtgt gggcacggta tgcgttgcac gaggattatc acgacacgat
      361 gaagccggcg ctggtggagg cggggcgggt gttggaggaa ctctacgggg tgacgagtgg
      421 cgactatcgt tattatgtgg atacggggcc ggtgttggag cggagctggg cggcgcgcgc
      481 gggggttggt tttgtgggga agaacgcgat gctgatttcg cgcacgcatg ggaattggct
      541 gtttttgtcg gcgatgctgt gtcgcgtgcc gttgcaagcg gacgagccgg tgcggaagaa
      601 attcgtcgat gaagacgcgg gcgcgatcgg gctgctgtgc ggcaagtgca cgcggtgcat
      661 cgatgcgtgt ccgacgaacg cgattgtgaa gccgggcgtc gtcgatgcgc ggaaatgcat
      721 atcgtatcag acgatcgaga acaaaggcgt gattccgcgg gagctgcgtg cggggatcgg
      781 ccggcggatt tacggctgcg atgtgtgtct cgaggtgtgt ccgtggaatc gtttcgcgcg
      841 cgaaggccgc gcgatgttgc tggtggcgcg cggcgatctg gcggggatgg cgggggcgga
      901 aattttagcg ctgacgccgg agacgtttgc ggaacgcttt cggcggacgc cgatcaagcg
      961 gttgaaactg gcgggattgc tgcgcaacgc gtgcattgtt gcggggaacg cgcgtgacaa
     1021 aagtttgctg ccgcaattgg tgacgctcac gcggcacgcg ctgccgctgg tgcgcgcgca
     1081 tgcggtttgg gcggtgtttc agatcgcggg gcaaacgcgc gcgacggaat tgctggcgga
     1141 ggcgcgtgcg gctgaaacgg cgggcgaggt gctggccgag tatgcggcgg aagagaggga
     1201 gaggggcgac aacggtggga tcgaaagacc ccgccctaca ggagaatagt ttagttgtcg
     1261 gggaaca
//
LOCUS       DASPLT010001312         1170 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_39608, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001312 DASPLT010000000
VERSION     DASPLT010001312.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1170)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1170)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1170
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_39608"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..459)
                     /locus_tag="VEA63_13415"
     CDS             complement(<1..459)
                     /locus_tag="VEA63_13415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007360151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HYD85055.1"
                     /translation="MKLRPLTGTITALVTPFRQGEIAYDDLAKLVAYQIKGGINGVVP
                     VGTTGESPTLSHEEHLEVIRATVAAARGRVPVIAGTGSNSTHEAAELTRLAHAAGADA
                     MLVVAPYYNKPSQEGLFRHYCAVADATDRPIILYSIPGRCGIEIGVPVVER"
     gene            529..>1170
                     /locus_tag="VEA63_13420"
     CDS             529..>1170
                     /locus_tag="VEA63_13420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HYD85056.1"
                     /translation="MTPVTPHTEIINDLLKLAVDSGVSDVIIKSNKPGYVRLSGKLKP
                     VEMDPITSPEAQAWVDEHVPKIFKQKWEDVGQIDFAYAVEGIGRFRVNAFHQRGLVSI
                     VLRHIKSTVPSFDQLGLGQTADALVKLVQAKDGILLVCGATGSGKSSTMAAMLNWVNQ
                     NQDKHIVTIEDPIEYTFLDDKSLFQQREIGLDVPSFDLAIKAVLRQNPDIILIG"
BASE COUNT          250 a          334 c          379 g          207 t
ORIGIN      
        1 gcgctcgacg acgggcacgc cgatctcgat gccgcaacgc ccggggatcg aatagagaat
       61 gatcggccgg tccgtggcgt cggcgacagc gcagtaatgg cgaaacaaac cctcctgact
      121 cggcttgttg taatacggcg cgacgaccag catcgcgtcc gcaccggcgg cgtgagcgag
      181 ccgtgtgagt tcggcggctt cgtgcgtgga gttggatccg gtgcccgcga tgaccggcac
      241 gcgaccgcgc gcggcggcga ccgtggcgcg gataacctcc aagtgctcct cgtgactgag
      301 cgtgggcgac tcgccggtcg tgccgacggg aacgacgcca ttgatgcctc ccttgatttg
      361 gtaagccacg agtttcgcga gatcgtcgta agcgatttcg ccctggcgaa agggagtaac
      421 gagagcggtg atcgtgccgg tgagcggacg gagtttcatt cgtgcgggca aagtagcctt
      481 gctgaacaaa gcagggcgaa ttacagaatc cgcaacgctt ttttacccat gacgcccgtc
      541 acaccgcaca cggaaataat caacgatttg ctcaagctcg cggtggatag cggcgtcagc
      601 gacgtgatca tcaaatcgaa caaacccggc tatgttcgcc tctccggcaa actcaaaccg
      661 gtcgagatgg acccgatcac gagccccgaa gcgcaggcgt gggtcgacga gcacgtgccg
      721 aagattttca agcagaagtg ggaggatgtc gggcagatcg acttcgccta cgcggtggag
      781 ggcatcgggc gttttcgcgt gaacgcgttt caccaacgcg ggctcgtgag catcgtgttg
      841 cgccacatca agagcaccgt gccgagcttt gaccaactcg ggctcgggca aacggccgat
      901 gcgctggtga agctcgtgca ggcgaaagac ggcatcctgc tggtgtgcgg cgcgacgggt
      961 tcggggaaaa gttcaacgat ggcggcgatg ttgaactggg tgaaccagaa ccaggacaag
     1021 cacatcgtca cgatcgaaga cccgatcgaa tacacgttcc tggacgacaa atcgcttttc
     1081 cagcagcgcg agatcggact cgacgtgccg agcttcgatt tggcgatcaa ggcggtgctg
     1141 cggcagaatc ccgacatcat cctcatcggt
//
LOCUS       DASPLT010001313         1096 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_96725, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001313 DASPLT010000000
VERSION     DASPLT010001313.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1096)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1096)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1096
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_96725"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1096
                     /locus_tag="VEA63_13425"
     CDS             <1..>1096
                     /locus_tag="VEA63_13425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HYD85057.1"
                     /translation="GEYAEWYWQRVESGKPEDAVWREFHAKNFGADFDYREFAPRFTA
                     ELFDAKQWARVFARAGVKYVVPTSKHHEGFALWPSAEASRTWGRPWNAAEIGPKRDLM
                     RELADATRAEGLKFGFYFSLYEWFNPLWKTDRARYVTEHMVPQFKDVVTRYEPAIIFS
                     DGEWDMTSKEWRSEELLAWLFNETAVKDEVVVNDRWGSETRHKHGGYWTTEYAAGLQD
                     DAHAWEESRGMGFSYGLNRAERIDDYKSARELVFVLIDLVSRGGNLLLDIGPAADGTI
                     PPIMEERLLEIGAWLNVNGEAIYGTRFAGRACQWTEGERPEQAFGEYRVKYELMEQIG
                     QAPREGRAVKQVFFTKKPDSLYAIAAGWPGE"
BASE COUNT          205 a          229 c          457 g          205 t
ORIGIN      
        1 ggggaatacg cggagtggta ttggcaacgc gtcgagagcg gaaagccgga ggacgcggtg
       61 tggcgggagt ttcacgcgaa aaattttggt gcggacttcg attaccggga gtttgcgccg
      121 cggttcacgg cggagctgtt cgatgcgaag cagtgggcgc gggtgtttgc gcgggcgggg
      181 gtgaagtatg tcgtgccgac gtcgaagcat cacgaagggt tcgcgttgtg gccgagtgcg
      241 gaggcgagtc gcacgtgggg gcgtccgtgg aacgcggcgg agattggtcc gaagcgcgat
      301 ctgatgcgcg agctggcgga cgcgacgcgg gcggaagggt tgaagttcgg tttctatttt
      361 tcgctctacg aatggttcaa tccgctgtgg aaaacggatc gcgcgcgtta tgtgacggag
      421 cacatggtgc cgcagttcaa ggacgtggtg acgcgctatg agccggcgat catttttagc
      481 gatggcgagt gggacatgac gtcgaaggag tggcgaagcg aagagctgct ggcgtggttg
      541 ttcaacgaaa cggcggtgaa ggatgaggtg gtggtgaacg accggtgggg cagcgagacg
      601 cggcacaagc acggcgggta ttggacgact gaatacgcgg cgggtttgca ggacgatgcg
      661 cacgcgtggg aggagagccg cgggatggga ttttcgtatg ggttgaaccg cgcggagcgg
      721 atcgacgatt acaagagcgc gcgcgagctg gtgttcgtgc tgatcgattt ggtgagtcgc
      781 ggcggcaatc tgttgttgga catcgggccg gcggcggacg ggacgattcc gccgatcatg
      841 gaggagcggt tgctggagat cggcgcgtgg ctgaatgtga acggcgaggc gatttatggg
      901 acgcgattcg cggggcgagc gtgtcagtgg acggaaggcg agcggccgga gcaggcgttt
      961 ggcgagtatc gcgtgaagta tgaattgatg gagcagatcg ggcaggcgcc gcgggaagga
     1021 cgggcggtga agcaggtgtt tttcacgaag aagccggatt cgctttatgc gatcgcggcg
     1081 ggctggccgg gcgagc
//
LOCUS       DASPLT010001314         2099 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_55002, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001314 DASPLT010000000
VERSION     DASPLT010001314.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 2099)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2099)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2099
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_55002"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(634..1167)
                     /locus_tag="VEA63_13430"
     CDS             complement(634..1167)
                     /locus_tag="VEA63_13430"
                     /inference="COORDINATES: protein motif:HMM:NF024833.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YXWGXW repeat-containing protein"
                     /protein_id="HYD85058.1"
                     /translation="MNTLRLQPQACVLLLLVTALGAGCSSSPSNTQRGVAGGAALGAL
                     AGAVIGNNRGSGNAASGAAIGAAAGAIAGGAIGHNADKRQRNATTPTSQDPNATNIYV
                     QGSPPLPPAAHAESVTARPTDEAVWIPGYWSYENGRDYAWVAGHWEIPPSGYRTFQPP
                     EWRRQGDGYVYVRGHWR"
     gene            complement(1316..2047)
                     /locus_tag="VEA63_13435"
     CDS             complement(1316..2047)
                     /locus_tag="VEA63_13435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009511743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HYD85059.1"
                     /translation="MGRQWLHAKRAIVNLKKGQVVGKLVKEMTVAAELGGADIAANAR
                     LFAAVEKAKKASVKADVIERAIAKGAGTGGDKSAMEHVVFEGYAPHKVPVIVEVYTDN
                     HQRTAPEIRVLFKKGVLGTAGSNKFLFEHVGIVEAHHPDATADREAAAIEAGANDFEP
                     LTHEQNDDIPENHAGARFITERTAVHAVSTWLKQNGWAVITSEIGYLAKTFPALDDAA
                     RAEVGEFLQALEDHDDVQRVWAAVK"
BASE COUNT          333 a          619 c          735 g          412 t
ORIGIN      
        1 attcgcgtcg accggtccgc ggcaaatttc gccgcaacca aatgtagcga ccgttgcgcg
       61 gcccagtccg cgtccggcgg tgcaagcgcc gccggtggtc cggtcagcac ccgctgtgcg
      121 ttcgccggag cgagtcgagc gttcgaatca tcatcatgtt gagcgttcca gtccttcgca
      181 cggcggggag cgaactcgtg cggatccggg gcgtggtcga ggcaatggtc gcggtggccc
      241 ggatcgctga gtggctcact cgctgcgaca tcgattcgca atcgggcgga aaaacacagt
      301 tagcgcggcg actgccgacg ctggaccggc gaaacgggtt gtccctcgtg ccatggcgtt
      361 ttcaaaaaag ctgtggccgt tttggaggga cgacctccgt gtcgtccgcg gccggcacgg
      421 cttcggtttg gctgaaaagg cggtagcact tgcgcgtgtc gcgtgcggtt ggatgtcgca
      481 tatgcgacat ccaactgcac gggtatacct gggctggtca cggcagagag ccggggaaac
      541 atcgggcgct gcaatcagcg cgcggccgcc tgcaagcagg ctcctgcctt cggcgagaat
      601 cgcgaggtgg cgataggaac gcgggtgctt cgtttagcgc cagtgcccgc ggacgtaaac
      661 atagccatcg ccttggcgac gccattcggg cggttgaaag gtgcggtagc cggacggtgg
      721 aatttcccaa tggcctgcga cccacgcgta gtcgcgaccg ttttcgtaag accaataacc
      781 gggaatccaa acggcttcgt cggtggggcg cgcggtgacg gattcggcat gcgcagcggg
      841 cgggagcggc ggcgagccct ggacgtaaat gttcgtggcg ttgggatcct gggaggtggg
      901 cgtggtcgcg ttgcgctgac gtttgtcggc gttgtgaccg atggcgccgc cggcaatcgc
      961 accggcggcg gcaccgatgg cggcgccgct cgcggcgttg ccgctgccgc gattgttgcc
     1021 gatgacagcg ccggcaagtg cgccgagtgc ggcgcctccg gcaacgccgc gctgggtgtt
     1081 cgatggactg ctcgaacagc ccgcaccgag tgcggtaacg agcaacaaga gcacgcaagc
     1141 ttgcggttgg agacgaaggg tgttcatgga tgaacgcctt agtcccgcag gaaaatgcgg
     1201 ctgttcctag ggtctgggcc cgatgaccgg ctgagcgatc gaccgatttc ggcgccgcag
     1261 attcgggtgc cggaaatccg tcgctgcgag tgtgcggcgg tgccgcgggt gaaatttatt
     1321 tcaccgccgc ccagacgcgt tgcacgtcgt cgtgatcttc gagcgcttgc aggaattctc
     1381 cgacttcggc gcgcgcggcg tcgtcgagcg cgggaaacgt ttttgcgaga taaccgatct
     1441 cgctcgtgat gacggcccag ccgttttgct tgagccaagt cgagacggcg tgaacggcgg
     1501 tgcgctcggt gatgaagcgc gcgccggcat ggttttccgg gatgtcgtcg ttctgctcgt
     1561 gcgtgagcgg ttcgaaatcg ttcgcgccgg cttcgatcgc ggcggcttcc cggtcggcgg
     1621 tggcgtcagg gtggtgcgct tcgacgatac cgacgtgttc gaagagaaac ttgttgctgc
     1681 cggcggtgcc gaggacgccc ttcttgaaga gcacgcgaat ctcgggcgcc gtgcgctgat
     1741 ggttgtcggt gtagacctcg acgatcacgg ggaccttgtg cggcgcgtaa ccttcgaaca
     1801 ccacgtgctc catcgcgctt ttgtcgccgc cggtgcccgc gcctttcgcg atcgcacgct
     1861 cgatgacgtc ggctttgacg ctggcctttt tggctttttc gaccgcggcg aacaatcgcg
     1921 cgttcgcagc gatgtcggcg ccgccgagct ctgcggcgac ggtcatttcc ttcacgagtt
     1981 taccgacgac ctgacccttt ttgaggttca cgatcgcgcg cttagcatgg agccattgac
     2041 gtcccataag ccgccgacgc tgacaatcgg cacggcggcg cgcaatgtcg aagcgggtc
//
LOCUS       DASPLT010001315         1953 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_55004, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001315 DASPLT010000000
VERSION     DASPLT010001315.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1953)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1953)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1953
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_55004"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            118..546
                     /locus_tag="VEA63_13440"
     CDS             118..546
                     /locus_tag="VEA63_13440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HYD85060.1"
                     /translation="MKYIPLGEYEHGVELVKIYECLCDVTRLRLLHVLAQGPLCVCHF
                     QAVLREPQVKISKHLGYLRERGLVECRREGNWVVYALPVKPGRELRANLACLQDCARE
                     QAIFRRDLERLQKLAPGIAADGPCGCGPAAARKRVSHDQR"
     gene            533..997
                     /locus_tag="VEA63_13445"
     CDS             533..997
                     /locus_tag="VEA63_13445"
                     /EC_number="1.20.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009513879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030612 - arsenate reductase (thioredoxin)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase ArsC"
                     /protein_id="HYD85061.1"
                     /translation="MTNAKPTILILCTGNSCRSHLAEGVLRAAAGDMFEVASAGSKPA
                     GYVHPLAIRAMAEIGVDISMHRSKHLSEFLNRDVETVITVCGHADQACPIFPGQVNRH
                     HWPFDDPAHATGTEEEQFAVFRRVRDEIRRRFEAYAAGRRDGLKAATSGSAR"
     gene            994..>1953
                     /gene="arsB"
                     /locus_tag="VEA63_13450"
     CDS             994..>1953
                     /gene="arsB"
                     /locus_tag="VEA63_13450"
                     /inference="COORDINATES: protein motif:HMM:TIGR00832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008490 - arsenite secondary active
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACR3 family arsenite efflux transporter"
                     /protein_id="HYD85062.1"
                     /translation="MKAEKQSVLERLSFLDRYLTLWIFLAMAAGLLAGRVAPWIAEVL
                     TDWSVGTTSVPIAIGLIVMMYPPLAKVRYEELGDVFRDKKVLGLSLVQNWLVGPLLMF
                     ALAAIFLRDRPEYFAGLVLIGLARCIAMVIVWNDLAGGSREYCAGLVAFNALFQVLFF
                     APMAWLFLNVLPPLAGMELGELDLSGVTIGSVAESVFIYLGIPFFAGWLTRWALRRGG
                     RRAWYDEKFVPAISPLTLAALLFTIFVMFSLKGDAVLRIPLDVLRIAVPLAAYFAIMF
                     FATFWMSVRAGTDYERAATLAFTAAGNNFELAIAVAVGVFGLAS"
BASE COUNT          287 a          457 c          777 g          432 t
ORIGIN      
        1 tcgagttcga ccggatgggc aaggtgatcg attgggagcg gcggccggcg gggaagcgtt
       61 gcgtgtgctg acgtgcggtg ggcgcgggtg acgatcgggt gacggtcgga tttgggcttg
      121 aagtatattc ctttagggga atatgaacac ggcgtggaat tggtgaagat ttacgaatgt
      181 ctgtgcgacg tgacgcggct gcggctgttg cacgtgctcg cgcaaggacc gctatgcgtg
      241 tgtcattttc aagcggtgtt gcgggagccg caggtgaaga tttcgaagca tctcggttat
      301 ctgcgcgagc gggggctggt ggaatgccgg cgcgagggaa actgggtggt ctatgcgttg
      361 ccggtgaaac cggggcggga gttgcgggcg aatctggcgt gcttgcagga ttgcgcgcgg
      421 gagcaggcga tttttcggcg agacctcgag cggctgcaaa agctggcgcc gggcatcgca
      481 gccgacgggc cgtgcggctg tgggccggcg gcggcgcgaa aacgtgtttc tcatgaccaa
      541 cgctaaaccg acgattttga ttctttgcac gggcaactcg tgtcgcagtc atctcgccga
      601 aggcgtgttg cgcgcggcgg cgggcgatat gttcgaggtg gcgagcgcgg gatcgaaacc
      661 ggcgggttac gtgcatccgc tggcgatccg cgcgatggcg gagatcggcg tcgacatctc
      721 gatgcaccgc tcgaagcacc tgagcgagtt tttgaatcgc gacgtggaaa cggtcatcac
      781 ggtgtgcgga cacgccgatc aggcgtgtcc gatttttccg ggacaggtga ataggcatca
      841 ctggccgttc gacgatccgg cgcatgcgac gggcacggag gaggagcagt tcgcggtgtt
      901 tcggcgggtg cgagacgaga tccggcggcg cttcgaggcg tatgctgcgg gtcggcgcga
      961 tgggctgaag gcggcgacgt cggggtcggc gcgatgaagg ccgaaaaaca gtcggtcctc
     1021 gagcggcttt cgtttttgga tcggtatctg acgttgtgga tttttctggc gatggccgcg
     1081 ggcttactgg ccgggcgagt ggcgccgtgg atcgcggagg tgttgacgga ttggagtgtt
     1141 ggcacgacgt cggtgccgat cgcgatcggg ctgatcgtga tgatgtatcc gccgctggcg
     1201 aaggtgcgct acgaggagtt gggcgacgtg tttcgcgaca agaaggtgct ggggctgtcg
     1261 ctcgtgcaga actggctcgt cgggcctttg ctgatgtttg cgctcgcggc gattttcctt
     1321 cgggaccggc ccgagtattt cgcggggctc gtgctgatcg ggctggcgcg gtgcatcgcg
     1381 atggtgatcg tgtggaacga tctggcgggc gggagccggg agtattgcgc ggggctggtg
     1441 gcgttcaacg cgttgtttca ggtgctgttt ttcgctccga tggcgtggtt gtttttgaac
     1501 gtgctgccac cactcgcggg catggagctg ggcgagctgg atttatcggg cgtgacgatc
     1561 gggagcgtgg cggagagcgt gttcatttat ctgggaattc cgttttttgc gggatggctg
     1621 acgcgctggg cgctgcggcg gggcgggcgg cgcgcgtggt atgacgaaaa gtttgtgccg
     1681 gcgatcagtc cgctgacgct cgcggcgctg ctgttcacga ttttcgtgat gttcagtttg
     1741 aagggggacg cggtgctgag gattccgctc gatgtgctgc gcatcgcggt gccgctggcg
     1801 gcgtatttcg cgatcatgtt tttcgcgacg ttctggatga gcgtgcgggc ggggacggat
     1861 tacgagcggg cggcgacgct ggcgttcacc gcggcgggaa ataatttcga gctggcgatt
     1921 gcggtggcgg tgggggtgtt cgggttggcg tcg
//
LOCUS       DASPLT010001316         1062 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_261218, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001316 DASPLT010000000
VERSION     DASPLT010001316.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1062)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1062)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1062
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_261218"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            15..>1062
                     /locus_tag="VEA63_13455"
     CDS             15..>1062
                     /locus_tag="VEA63_13455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD85063.1"
                     /translation="MRYEALAQPFAGAKPRDDFWIALLTNRAELAGELHLFEDAWSLR
                     DRVADFLAGQPPSPETARRYIDFLQRALPDQMQARGPAFEAFRTHFVHGARVGLTADD
                     RAWCQWQIARLQPAMASESTTSPRLALRERAALWSAALAAAAGTRWLSFVRAEEFIWR
                     TSSRWSPEAAPDTPADIPALLTELRAIRETLPPTPGPNANSIRAELHAIENSLEDPSL
                     IVHLGSLVAPGDPVAIAFGASGLASLAFTLEQLPPEEWIALELPNTSVPPDSRNVHRS
                     ARARQVREWSIPLPDSAQLAWHSQIVEAAPSLKPGTYLLTVTGHPRAPAKRVTWQGRF
                     IVSSLRGLAQFSSAT"
BASE COUNT          159 a          400 c          303 g          200 t
ORIGIN      
        1 ccgaccttca gttcgtgcgc tacgaggcac tcgctcaacc gttcgccgga gcaaaaccgc
       61 gcgacgattt ctggatcgcg cttctcacga atcgcgcgga gctcgcaggt gaacttcacc
      121 ttttcgagga cgcttggtcg ctgcgcgacc gtgtcgcgga ttttctcgcc gggcagcctc
      181 cgtcgccgga aacagcccgt cgctacatcg actttctgca acgggcgttg cccgatcaga
      241 tgcaggcgcg cggccctgcg ttcgaagcgt ttcggacgca tttcgttcac ggcgctcgcg
      301 tcggcctcac ggctgatgat cgcgcctggt gtcagtggca gatcgcgcgc ctgcagcctg
      361 cgatggcatc cgagtccacg accagcccgc gcctggccct ccgcgaacgc gccgcgctct
      421 ggtcagccgc gctcgccgcg gccgccggca cgcgatggct ctccttcgtt cgggccgaag
      481 aattcatctg gcgaacctcc agccgttggt cgcccgaagc tgcgccagat acgcccgcgg
      541 acatccccgc gcttttgacc gagctccgcg cgatccgcga aacgcttccg cccacgcctg
      601 gcccgaatgc caactccatc cgcgccgaac ttcacgcaat cgaaaacagc ctcgaagatc
      661 cttccctcat cgttcacctc ggctcactcg ttgcacccgg cgatccggtt gcaatcgcgt
      721 tcggcgcgtc ggggctggca tctctcgctt tcactctgga gcaattgccg cccgaggagt
      781 ggatcgcgct cgagcttccc aacacttctg tgccaccgga ttcccgcaac gtgcaccgct
      841 ccgcccgcgc gcggcaggtt cgcgagtggt cgatcccgct gcctgactcc gcacagctcg
      901 cgtggcactc gcaaatcgtc gaagccgccc cgtcgctcaa acccgggacg tatctgctca
      961 ccgtgaccgg tcatccgcga gctccggcga aacgcgttac ctggcagggc cgcttcatcg
     1021 tctcctcgct tcgcggcctc gcgcagttct ccagtgcgac ag
//
LOCUS       DASPLT010001317         2791 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_117251, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001317 DASPLT010000000
VERSION     DASPLT010001317.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 2791)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2791)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2791
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_117251"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1030)
                     /locus_tag="VEA63_13460"
     CDS             complement(<1..1030)
                     /locus_tag="VEA63_13460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007361528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit alpha"
                     /protein_id="HYD85064.1"
                     /translation="MNTTRTSDTGPTHPRSSSAPSRGTPKNLRPAKQGLYDPWFEHDA
                     CGVGFVVDMKGRKTHTILQQGLQVLKNLDHRGASGAEVNTGDGAGILLQMPHKFFAEV
                     SKKARISLPETGHYGAGLVFLPRNPTSRRKLEEVFAGIIQSEGQTLLGWRTVPTDNSM
                     LGDTAKSAEPFMRQVFIGRAAGLDDAAFERKLYVIRKRAYSEIRTSTFSGAETWYVPS
                     LSFKTIVYKGMLTTEQLEQYFTDLKHPSMETAIALVHSRFSTNTFPSWDRAHPYRYIA
                     HNGEFNTLRGNINWMHARQALFNSELFGEDIAKILPIINPNGSDSSMFDNTLELLVLA
                     GRPLAHAMM"
     gene            complement(1198..1533)
                     /locus_tag="VEA63_13465"
     CDS             complement(1198..1533)
                     /locus_tag="VEA63_13465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007361530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin"
                     /protein_id="HYD85065.1"
                     /translation="MKIKAYLKPSCGWSNGVRAIMRKYSLPFEDIDIINNRANYAEMV
                     SKSGQPLSPCVEIDGVMLADVSGEEVENYLLSNDLIKPVDTPVNVPTNAGCSDEEHAR
                     MATKTVRFF"
     gene            1816..>2791
                     /locus_tag="VEA63_13470"
     CDS             1816..>2791
                     /locus_tag="VEA63_13470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HYD85066.1"
                     /translation="MKVLVTGAAGFIGYHVARRLAETKRCEVLGIDNLNDYYSVDLKR
                     ARLAELEPLENFRFVQADFADAKAFDGIYTHFKPQYVVHLGAQAGVRYSLENPAAYAR
                     SNLDGFLNVLEACRKAPPKHLVFASSSSVYGASAQVPFSEDAPTDQPISFYAATKKSN
                     ELMAHSHAHLHGLAITGLRFFTVYGPWGRPDMAPTLFAKAICEGRPITLFNHGQNRRD
                     FTYVDDIVDGIVKVLLYPPPGDAPLAAPAEISRVARAAGLVAPTHESAATPRGLFRIF
                     NIGHHRSVEVLLFVRMLEELLGKKALVELAPPQPGDMIETCANIDRLHH"
BASE COUNT          521 a          836 c          892 g          542 t
ORIGIN      
        1 tcatcatcgc atgcgcgagc ggccggccgg cgagaaccag gagctcgagc gtgttgtcga
       61 acatcgacga atcggagccg ttcgggttga tgatcggcag gatcttcgcg atgtcctcgc
      121 cgaagagctc gctgttgaac agcgcttggc gggcgtgcat ccagttgatg ttgccgcgca
      181 gcgtgttgaa ttcgccgttg tgcgcgatgt agcgatacgg atgcgcacgg tcccagctcg
      241 ggaaggtgtt cgtcgagaag cgcgagtgga cgagcgcgat cgcggtctcc atcgacgggt
      301 gcttcaggtc ggtgaaatac tgctcgagct gctccgtcgt gagcatgcct ttgtagacga
      361 tcgtcttgaa cgagaggctc gggacatacc acgtctccgc gcccgagaac gtcgaggtgc
      421 ggatctccga gtaagcgcgc ttgcggatga cgtagagctt ccgctcgaac gcggcgtcat
      481 cgaggccggc ggcgcggccg atgaacacct gccgcataaa cggctccgcg gacttcgcgg
      541 tatcgccgag catcgagttg tcggtcggaa cggtgcgcca gccgagcagc gtctggcctt
      601 cggattgaat gatgccggcg aacacttcct cgagcttgcg ccgggaggtg ggattgcgcg
      661 gaaggaaaac gaggccggcg ccgtagtggc cggtttcggg gagcgagatg cgcgccttct
      721 tcgagacttc cgcaaagaac ttgtgcggca tctgcagaag gatgcccgcg ccgtcgccgg
      781 tgttcacctc cgcgccgctg gcgccgcggt gatcgaggtt cttgaggacc tgcagaccct
      841 gctgaaggat cgtgtgggtc ttgcgcccct tcatgtcgac cacgaagccg acgccgcacg
      901 catcgtgttc aaaccacggg tcgtagagac cttgtttggc gggacgaagg tttttcggcg
      961 tgccgcgcga gggggcgctg ctgctgcgag ggtgagttgg gccggtgtcc gaggtccgcg
     1021 tggtgttcac aatggtgtgt ctggttgaga tcagctatac gagaaacaga tgacgcccac
     1081 gagacggctc gcgtggcggc aggagatgcg ggagagaagg gggagcataa aaaaggccgg
     1141 tctcgtcgtt gcgagagcgg cccggagtga gttagcagcg gttggctctc agcagcatca
     1201 gaagaagcgg accgttttcg tcgccattct cgcgtgctct tcatcagagc agccggcgtt
     1261 cgtcggcacg ttcacggggg tatccacagg ttttatcaga tcgttgctga gcaaataatt
     1321 ttcgacttcc tcgccggaca cgtcggcgag catcacgcca tcgatctcga cgcagggcga
     1381 caacggctgg cccgacttcg aaaccatctc ggcgtaattc gcgcggttgt tgatgatgtc
     1441 gatgtcctcg aacggcaggc tgtatttccg cataatggcg cgaacgccat tggaccagcc
     1501 gcaggagggc ttgagatagg ctttgatctt catgcaggaa acgtgtgcga gtgcaaaagc
     1561 gccgcaaggt ataggacggg tttttcggca atcaagcacc gtgttgggcg attcggaaaa
     1621 tttttcggag gcgactgtgc tggacagcgc cgcgcggcgc gaaagctcca agctccaacc
     1681 tccaagctcc agtgaatgtc caaacctcaa atgccaaatc accggcgcct tgtttagagc
     1741 ttggtgtttc attggagctt ggagcttgga atttggagct tcccgcgcgt cagcgcgggc
     1801 cctatcaccc gccgcatgaa agtcctcgtg accggtgcgg ccggtttcat cggctaccat
     1861 gtcgcacgcc ggctcgccga gacgaagcgc tgcgaggttc tgggtatcga taacctcaac
     1921 gactactatt cggtcgactt gaagcgcgcg cggctcgcgg agctggaacc gctggagaat
     1981 ttccgcttcg tgcaagccga cttcgccgac gcgaaggcct tcgacgggat ctacacgcat
     2041 ttcaaaccgc agtatgtcgt gcacctcggc gcgcaggccg gcgtgcgtta cagcttggaa
     2101 aaccccgcgg cttacgcgcg cagtaatctc gacggatttc tgaacgtgct cgaggcgtgt
     2161 cgcaaagctc cgccgaaaca cctcgtgttc gcctcgtcga gcagtgttta cggtgcgagc
     2221 gcgcaagtcc ctttcagcga agacgcgccg accgaccaac cgatctcgtt ctacgccgcc
     2281 acgaaaaaga gcaacgagct gatggcgcac agccacgcgc acttgcacgg gctcgcgatc
     2341 accgggctgc gcttttttac cgtctacgga ccttggggcc ggccggacat ggcgccgacg
     2401 ctgttcgcca aagccatctg cgagggccgg ccgatcacgc tcttcaatca cgggcaaaac
     2461 cgccgcgatt tcacgtatgt cgacgacatc gtcgacggca tcgtcaaagt cctcctctac
     2521 ccgccgcccg gcgacgcgcc gctggcggca cctgcggaga tttcgcgcgt cgcacgcgcc
     2581 gccggtctcg tcgccccgac gcacgaatct gccgcaacgc cgcgcggttt gtttcgcatc
     2641 ttcaacatcg gccaccaccg gtccgtcgaa gtgttgctct tcgtgcgcat gctggaagag
     2701 ctcctcggca agaaagcgct cgtcgaactc gcgccgccgc aacccggcga catgatcgag
     2761 acctgcgcca acatcgatcg cctgcaccac g
//
LOCUS       DASPLT010001318         1568 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_39673, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001318 DASPLT010000000
VERSION     DASPLT010001318.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1568)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1568)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1568
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_39673"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..842)
                     /locus_tag="VEA63_13475"
     CDS             complement(<1..842)
                     /locus_tag="VEA63_13475"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007359969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HYD85067.1"
                     /translation="MARIVQKYGGTSVGDVDRIKNVASRIKAIRDAGNELVVVVSARA
                     GVTNELIARAKAVCGGAVPSEREMDQLLSIGEQETIALTAMALHGLGVEAVSYTGAQA
                     GIFTDTVHTKAKIKTINAKALEKDLVAGKVIIVAGFQGINDVGQTTTLGRGGSDLTAI
                     ALAAAIKADKCEIYTDVDGVYTADPRVVKTAKKLNEISYDEMLELASLGSKVMQSRSV
                     EFAAKYNVVFEVRSSFNNNPGTIVKQEVAYMEKVVVRGVAVDKDQAKVLVRDIVDKPG
                     SAARV"
     gene            complement(901..>1568)
                     /locus_tag="VEA63_13480"
     CDS             complement(901..>1568)
                     /locus_tag="VEA63_13480"
                     /inference="COORDINATES: protein motif:HMM:NF012945.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HYD85068.1"
                     /translation="TEKMIVEGIDRLTPADFHHAATFGYGIKLLAVIARDFTKNELSV
                     RVHPTLLPKSHVVANVNGVFNAVSVQGDVVGTTLYIGRGAGQDATSSAVISDIVGAVE
                     LLKNDRRAFLTARRELTLARPTGRCTLAPPERIHGQYYLRLTVKDRPGVLARIAAIMA
                     AQQVSIATVIQTTTDRPDVASLVLTTHDSNERAIQATIARLKKLPVVLEAPVLLRLAN
                     FKE"
BASE COUNT          267 a          541 c          440 g          320 t
ORIGIN      
        1 acacgcgccg ccgagccggg cttgtccacg atgtctctca cgagaacctt ggcctggtcc
       61 ttgtcgacag cgacgccgcg gacgacgact ttttccatgt aggcgacttc ctgtttcacg
      121 attgttcctg ggttgttgtt aaaggatgag cggacttcga aaaccacatt gtatttcgcc
      181 gcgaactcca ccgagcgcga ctgcatcacc ttgctgccga gcgaagcgag ttcgagcatc
      241 tcgtcgtagc tgatctcgtt caatttcttc gccgtcttca ccacgcgcgg gtccgccgtg
      301 taaacgccgt caacgtccgt gtagatctcg cacttgtcgg ccttgatcgc cgccgcgagc
      361 gcgatcgccg tcaggtccga gccgccgcga ccgagcgtcg tcgtctggcc cacgtcgttg
      421 atcccttgaa aacccgccac gatgatcacc ttcccggcga cgagatcctt ctccagcgcc
      481 ttcgcattga tcgtcttgat ttttgccttc gtgtgaacgg tgtcggtgaa aatccccgcc
      541 tgcgctcctg tgtaactcac cgcctccacg ccgaggccgt gcaacgccat cgccgtcagc
      601 gcgatcgtct cctgctcgcc gatcgacagc agttggtcca tctctcgctc gctcggaacg
      661 gcgccgccgc agaccgcttt cgcgcgcgcg atcaactcgt tcgtcacgcc ggcgcgcgcc
      721 gaaacgacaa cgacgagctc gttgcccgca tcgcggatcg ccttgatccg ggacgcgaca
      781 ttcttgatgc gatcgacgtc accgaccgag gtgccgccat atttttgaac gatccgagcc
      841 atgttctgtt gagagaaatg agagctgagt ggtgagagaa gcggcagcgt gagcgctcga
      901 ctattccttg aagttcgcga ggcgcaacaa caccggcgct tcgagcacca ccggcagttt
      961 cttcaaccgc gcaatcgtcg cctggattgc gcgctcgttg ctatcgtgcg tggtcaacac
     1021 gagcgacgcc acatcgggac gatccgtcgt cgtctggatc accgtcgcga tgctcacctg
     1081 ctgcgccgcc atgatcgccg cgattcgcgc gagcacgccc ggccggtcct tcaccgtgag
     1141 ccgcagataa tattggccat ggatccgctc cggcggcgcc agtgtgcagc ggccggtcgg
     1201 ccgcgcgagc gtcagctcac gtcgcgccgt caaaaacgcc cgccggtcgt tcttcaacaa
     1261 ctcgaccgcg cccacgatgt cgctgatcac cgcgctcgac gtcgcatcct gccccgcgcc
     1321 gcggccgatg tagagcgtcg tgccgacgac gtctccttgc accgacaccg cgttgaacac
     1381 gccgttgacg ttcgccacca cgtggctctt cggcagcagc gtcggatgca cgcgcacgct
     1441 caactcgttc ttcgtaaaat cgcgcgcgat gacggcgagc agcttgatcc cgtagccgaa
     1501 cgtcgccgca tgatgaaagt ccgcgggcgt caatcgatcg atcccttcga cgatcatctt
     1561 ctccgtcg
//
LOCUS       DASPLT010001319         2299 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_139403, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001319 DASPLT010000000
VERSION     DASPLT010001319.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 2299)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2299)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2299
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_139403"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..263)
                     /locus_tag="VEA63_13485"
     CDS             complement(<1..263)
                     /locus_tag="VEA63_13485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HYD85069.1"
                     /translation="MNRFLLRVLRFLSFVFGALLLVALLAGGWFYFQLRASLPQLDGT
                     QPLAGLDATVTITRDDHGVPTLRATTRRDVALALGFLHAQERF"
     gene            complement(375..887)
                     /locus_tag="VEA63_13490"
     CDS             complement(375..887)
                     /locus_tag="VEA63_13490"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR01313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046316 - gluconokinase activity [Evidence
                     IEA];
                     GO_process: GO:0046177 - D-gluconate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HYD85070.1"
                     /translation="MERLTARHAMVILVMGVAGSGKTTIGQLLARELRWSFADADDFH
                     PPANVAKMAAGQPLDDADRAPWLAALRAHIEGCLARNENAVVACSALKSAYRATLVVD
                     RTQVRVVHLHGSRELLQSRLQHRKGHFMRPEMLESQLATLEPPSHALTLDVSATPPEL
                     VARIRQAFAI"
     gene            complement(875..1327)
                     /locus_tag="VEA63_13495"
     CDS             complement(875..1327)
                     /locus_tag="VEA63_13495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5069 domain-containing protein"
                     /protein_id="HYD85071.1"
                     /translation="MPPASGLRSCYAKVGRLVYFGRMLDKIRLHATGRLPSDYHGNLG
                     IGFDGRTCSFLKVNYADLVARVQQGGTDEEILAWCHERGGGRSDDECNIWSRFMMKTG
                     WRDDRSAILRQRIVEYGLDGKNLTIETFFDLNDYDEGRDPVAAQSWNA"
     gene            complement(1405..2064)
                     /locus_tag="VEA63_13500"
     CDS             complement(1405..2064)
                     /locus_tag="VEA63_13500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HYD85072.1"
                     /translation="MADSLSIILADDHALVRAGIRTLLEKLPGVSIVAESGDGRETVA
                     LVHQRDPDIVIMDISMPELNGLDATARIVRECPRTKVLILSMHRGEDYVLQALRAGAT
                     GYLLKDAATAELQLALIAVRRGETYLSPTISKEVLARHRKQELDPKAETTKTLTPRMR
                     EIVQLIAEGRSTKEIAFLLNLSVKTIETHRMHLMARLDLHDVAGVVRYALRNGLISAE
                     K"
     gene            complement(2074..>2299)
                     /locus_tag="VEA63_13505"
     CDS             complement(2074..>2299)
                     /locus_tag="VEA63_13505"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HYD85073.1"
                     /translation="GLQDGHLRLGVRDDGIGFDAAAARVRAERGGSLGLLGMHERASL
                     AGGTLTLLSAPGRGTEVEAVFPLSNAVETH"
BASE COUNT          407 a          628 c          863 g          401 t
ORIGIN      
        1 aagaagcgtt cctgcgcatg gagaaagccg agcgcgagcg cgacgtcgcg gcgcgtggtc
       61 gcgcggaggg tgggcacgcc gtggtcgtcg cgcgtgatgg tgacggtggc gtcgagaccg
      121 gcgaggggtt gtgtgccgtc gagttgaggg agactggcgc gaagttgaaa atagaaccag
      181 ccgccggcga gcagtgcgac cagcagcagc gcaccgaaaa cgaaggagag aaagcgaagg
      241 acgcggagga gaaaccggtt catggcggaa gcgcgatttc cgaacgttaa cggcggacgt
      301 tcggtctgcc atcttttcga ccaggcgggg atgggcgtaa tatcgcctgc aagcaggctc
      361 ctacaatgag ttggttaaat ggcgaaagcc tggcggatgc gggcgacgag ttcgggcggc
      421 gtggccgaga catcgagcgt cagtgcgtgc gagggtggct cgagtgtggc gagctgactc
      481 tcgagcattt cggggcgcat gaagtgtcct ttgcggtgct gcaggcgtga ctgcagcagt
      541 tcgcgcgagc cgtgcaggtg aacgacgcgc acctgcgtgc gatcgacgac gagcgtcgcg
      601 cgataggcgg atttcagcgc ggaacaggcg acgacggcgt tttcgttgcg ggcgaggcag
      661 ccttcgatgt gcgcacgaag ggcggcgagc cagggcgcac gatcggcgtc gtcgagcggc
      721 tggccggcgg ccattttggc gacgttggcg ggcgggtgaa aatcgtcggc gtcggcgaag
      781 ctccaccgga gctcacgggc cagcaactgg ccgatcgtgg ttttgccgct gccggcgacg
      841 cccatgacaa ggatcaccat cgcgtgacgc gccgtcaggc gttccaagat tgcgcggcga
      901 caggatcgcg accttcgtcg taatcgttca gatcgaagaa agtctcgatg gtgaggtttt
      961 tcccgtcgag gccgtattcg acgatgcgct ggcggagaat ggcactccga tcgtcgcgcc
     1021 agccggtctt catcatgaaa cggctccaga tgttgcactc gtcgtcggag cgaccgccgc
     1081 cgcgctcatg gcaccacgcg agaatttcct catcggtgcc accttgttgg acgcgcgcga
     1141 caaggtcggc gtagttcacc ttgaggaagc tgcaggtgcg gccgtcgaaa ccgatgccga
     1201 gattcccgtg ataatcgctg ggcagccggc cggtggcgtg caggcgaatc ttgtcgagca
     1261 tgcggccgaa gtagacgagg cgtccaactt tggcgtagca actgcggagt ccggaggcgg
     1321 gaggcatgag ggcgggatga agtggtattc tgaatacgct aagcgaaacg aagcggcgag
     1381 aggagcgtgg cggtggacgc gcgtttattt ttccgccgag atcagaccgt tgcgcagggc
     1441 gtagcgcacg acgccggcga cgtcgtgcag atcgaggcgc gccatgaggt gcatgcggtg
     1501 cgtctcgata gttttcacgc tcaggttgag gaggaacgcg atttccttgg tgctgcggcc
     1561 ttcggcgatg agctgcacga tttcgcgcat gcgcggcgtg agcgtcttag tggtttcggc
     1621 tttgggatcg agctcctgct tgcgatggcg ggcgaggact tccttggaga tggtggggct
     1681 gaggtaggtt tcgccgcggc ggacggcgat gagtgcgagc tggagttcgg cggtggcggc
     1741 gtccttgagg agataaccgg tggcgccggc gcgcagcgct tgcagcacgt agtcttcgcc
     1801 gcggtgcatc gagaggatca ggaccttcgt gcgcgggcat tcgcggacga tgcgagcggt
     1861 ggcgtcgagg ccgttgagct cgggcatcga gatgtccatg atcacgatgt cgggatcgcg
     1921 ctggtgaacg agcgcgacgg tttcgcggcc gtcgccggat tcggcgacga tggaaactcc
     1981 cggaagcttc tcgaggagcg tgcggatgcc ggcgcgcacc agcgcgtggt catcggcgag
     2041 aattatcgag agcgagtcgg ccacgtccca atttcaatgc gtttcgacgg cgttggacag
     2101 cggaaaaacc gcttccactt cggtgccgcg gccgggcgcc gacaaaagcg tgagcgtgcc
     2161 gccggcgagc gaggcgcgct cgtgcatgcc gagcagcccg aggctgcccc cgcgttcggc
     2221 gcgcacgcgg gcggccgcgg cgtcgaaacc gatgccatcg tcgcgcacgc cgagccgaag
     2281 atgtccgtct tgcaagccg
//
LOCUS       DASPLT010001320         1399 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_239772, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001320 DASPLT010000000
VERSION     DASPLT010001320.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1399)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1399)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1399
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_239772"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..618)
                     /locus_tag="VEA63_13510"
     CDS             complement(<1..618)
                     /locus_tag="VEA63_13510"
                     /inference="COORDINATES: protein motif:HMM:NF014683.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015129 - lactate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015727 - lactate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HYD85074.1"
                     /translation="MPWPQIYSPVGGIGASALVAALPVITLLGLLAFFHVRAQIAALA
                     GLIVALAIAIFVYDMPATLAAASATYGAAYGLFPIGWIILNAIFIYQLSVDTGGFAVL
                     QKQIANVSGDRRIQSLLIAFSFGAFIEGAAGFGTPVAITGALLIGLGFKPLEAAKLAL
                     IGNTAPVAFGALGTPLITLARITDLDLMQLSAMVGRQLPFFSLIVP"
     gene            complement(1038..>1399)
                     /locus_tag="VEA63_13515"
     CDS             complement(1038..>1399)
                     /locus_tag="VEA63_13515"
                     /inference="COORDINATES: protein motif:HMM:NF021778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="beta-propeller fold lactonase family protein"
                     /protein_id="HYD85075.1"
                     /translation="EPFERVSTLPDDFSGKNTTSEIRIHPNGRFVYAANRGHDSIAVF
                     ERDAETGALTRVEIVPCGGANPRNFALTPDGAWLLCASQNSNNLTVFQVDAETGRLAA
                     TEHGAPASKVVCVLFLR"
BASE COUNT          292 a          446 c          438 g          223 t
ORIGIN      
        1 cggaacgatc agcgaaaaga acggcagttg ccggccgacc atcgcgctga gttgcatgag
       61 atcgaggtcg gtgatgcgcg cgagcgtgat gagtggcgtg cccagcgcac cgaacgcgac
      121 cggcgcggtg ttgccgatga gcgccagctt cgcggcttcg agcggcttga acccgaggcc
      181 gatcaacagt gcgcccgtga tcgcgacggg ggtgccgaaa ccggcggccc cttcgataaa
      241 cgcaccgaag ctgaaagcga tcagcagcga ttgaatgcgc cggtcacccg acacgttcgc
      301 gatttgcttt tgcagcaccg cgaaaccgcc cgtgtcgacg gaaagttgat aaatgaaaat
      361 cgcgttgagg atgatccagc cgatcggaaa caaaccgtag gcggcgccat acgtcgctga
      421 cgcagcggcg agcgtcgccg gcatgtcgta gacgaagatc gcgatcgcca gcgcaacgat
      481 caggccggcc aacgcggcga tctgcgcccg cacatggaag aacgcgagca gaccgaggag
      541 cgtgatcaca ggcaaagcgg cgaccaacgc agacgcgccg atcccgccga cgggggaata
      601 gatttggggc cagggcatgg ggacgaccga aggacgcagc cgataggggg ttggatgcgc
      661 gagtcgcgcc aaagcgaggc aagccccgcc cgccctagcg agcgttttgt gcaagcaaac
      721 cgcccgtgca cgatccagcc cggaaaacct acacgtagca aaaagcgtga gcttttcgtt
      781 tctcgcgcgg tcgaaacgct cacgcgtttc gctacagcac agctacagca ccctgcgcgc
      841 gccccgcctc cgcgatgatc tatgcaggct acagcatttc caaagaaaac cgtagccgct
      901 tctggaggga cgacctccgt gtcgtccgca gccggcacgg aggccggccc tccgtctgac
      961 cggctatggg tttctgcaaa cgctctcgct cgaacccgtg ggagtgcctc cggtcctcca
     1021 gtctcagcga agtctcgtca acgcaggaac agcacgcaaa ccaccttgct cgcgggcgca
     1081 ccgtgttccg tcgccgcgag ccgccccgtt tccgcgtcca cttggaagac cgtgagatta
     1141 tttgaattct ggctcgcgca cagcagccac gcgccgtccg gtgtgagcgc gaagttccgg
     1201 ggattggcgc caccacaagg cacgatttcg acgcgtgtca gcgcaccggt ttccgcgtcg
     1261 cgctcaaaga ccgcgatgct gtcgtgcccg cgattcgccg cataaacgaa gcgcccattc
     1321 ggatgtatcc gaatttcgga tgtcgtgttt ttcccgctga aatcgtccgg caaggtcgac
     1381 acgcgctcga aaggctcgg
//
LOCUS       DASPLT010001321         1597 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_180302, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001321 DASPLT010000000
VERSION     DASPLT010001321.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1597)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1597)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1597
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_180302"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1597)
                     /locus_tag="VEA63_13520"
     CDS             complement(<1..>1597)
                     /locus_tag="VEA63_13520"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HYD85076.1"
                     /translation="IGIVVDDAIVVVENVERHIREGETPFNAAILGARELVGPVISMT
                     ITLAAVYAPIAFQGGLTGALFREFAMTLAGAVAVSGFVALTLSPMMSAYLLKDRAHEE
                     VGLSGWTNHLFDRLRNRYEHAVGRLIQVREGGRISWSLLLWGAVIWIPFWPIALGIVW
                     AIFGSGAMKSAAIPLAIGLAAATFIGALLSLVFDLLLGRRYQSVQYALTGAMMLTLLI
                     VPFFMFAQSELAPKEDQGVVFSILLPSPTATIDQNILYGEEVQKMFTSVSEYENSFQL
                     IAAQFGFSGIILKPWSERSRTSLEIEQSLQAPAAAIPGMNVVITTPDPLPSGGSMPIE
                     FVMRSTADHLEMQEFANQLVLYANTEANAGGGAPTFYFADTDLKFDLPQVEIEIDKDK
                     VATMGLSLVDVARDLGSMLGGGYVNRFVNAGRSYRVIPQVERGERLNASQLLDYHIRG
                     PQGELIPLSTIATLKETVQPRTLNRFQQLNSVKITGVGPSIDNALKKLEAKAAEILPA
                     GYSIDYGGQSRQLRYEGKALWQAA"
BASE COUNT          324 a          522 c          483 g          268 t
ORIGIN      
        1 cgcggcctgc caaagcgcct tgccctcata gcggagctga cgcgactgac cgccgtaatc
       61 gatcgagtaa cccgccggca aaatctcggc ggccttcgct tcgagcttct tcagcgcgtt
      121 gtcgatgctc gggccgacgc ccgtgatctt caccgagttc aactgctgga agcggttcag
      181 cgtgcgcggc tgcacggttt ccttgagcgt cgcgatcgtc gacagcggga tcaattcccc
      241 ctgcgggccg cgaatatgat aatcgagcag ctgcgaggcg ttcaaccgct cgccgcgctc
      301 gacctgcggg atcacccgat agctgcggcc ggcgttgacg aagcggttga cgtaaccgcc
      361 gccgagcatg gaaccaagat cgcgcgcgac gtcgacgagg ctcaggccca tcgtcgccac
      421 cttgtccttg tcgatctcga tctccacctg cggcagatcg aacttcagat cggtgtcggc
      481 gaagtaaaac gtcggcgcgc cgccgcccgc attcgcctcg gtgttggcgt aaaggacaag
      541 ctggttggcg aattcctgca tttcgaggtg atccgccgtc gagcgcatga cgaattcgat
      601 cggcatcgat ccgccggagg gcagcgggtc gggcgtcgtg atgacgacat tcatgccggg
      661 gatcgccgcc gccggcgcct gcagcgactg ctcgatttcg agcgacgtgc gggagcgctc
      721 cgaccagggc ttcaagatga tgccggaaaa cccgaactgc gcggcgatca gctgaaacga
      781 attctcatac tccgagacgc tcgtgaacat cttctgcact tcctcaccat agaggatgtt
      841 ctggtcgatc gtcgcggtgg gcgacggcag gaggatgctg aacaccacgc cctgatcctc
      901 tttcggcgcc agctcgcttt gggcaaacat gaaaaagggc acgatcaaga gcgtgagcat
      961 catcgcgccg gtgagcgcgt attgaaccga ttgataccgg cggccgagca gcagatcgaa
     1021 gaccaggctg agcagcgcac cgataaacgt cgccgccgcc agcccgatcg ccagcgggat
     1081 tgccgccgac ttcatcgcgc cgctgccgaa aatcgcccac acgatgccga gcgcgatcgg
     1141 ccagaacgga atccagatca ccgcgcccca aagcagcagg ctccatgaaa tgcgaccgcc
     1201 ttcacgcacc tgaatgagcc ggccgaccgc gtgttcgtag cgattgcgca gccggtcgaa
     1261 gaggtgattg gtccagccgc tcaggccgac ttcctcatgc gcacgatcct tcaacagata
     1321 cgcgctcatc atcggcgaaa gcgtcagcgc cacgaagccg gaaaccgcga ccgcgccggc
     1381 gagcgtcatc gcgaactcgc gaaacaacgc accggtgagg ccaccttgaa aagcgatcgg
     1441 cgcgtaaacc gcggcgagcg tgatcgtcat cgaaataacg gggcccacca gctctcgagc
     1501 gccgagaatc gccgcgttga acggcgtctc gccctcgcga atgtgacgct ccacgttttc
     1561 gaccacgaca atcgcgtcgt cgacgacgat gccgatc
//
LOCUS       DASPLT010001322         1210 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_55036, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001322 DASPLT010000000
VERSION     DASPLT010001322.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1210)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1210)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1210
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_55036"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..918)
                     /locus_tag="VEA63_13525"
     CDS             complement(<1..918)
                     /locus_tag="VEA63_13525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009508619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HYD85077.1"
                     /translation="MAFPTPPAILTARDLRAAELENPSRDTLLRLYTWMQLARTGDNR
                     NLDLFRQGLMKGTLAGGQGNEGLIVPLTLLADKAIDVVSFTHRDFGGHLIWSGHLCDH
                     LNQYFANAGSPTRAREGNVHRGDPKNRSFPMISHLGAMTSTVLGGADSQRRLGRPAVG
                     FALFGDGGSSTGDIHESLNLASLLSLPILFIIENNCYAYSTPTCEQFCEGTELWRRAA
                     GYGMEGFAVDCTTDVVGTARTFAAAIEKVRTTSRPILIEAHTLRLRGHAAYDTCNYLK
                     PVEADAFVARDPLPKFRASLVAAGLGAQLD"
BASE COUNT          228 a          296 c          484 g          202 t
ORIGIN      
        1 gtcgagttgc gcgccgaggc cggcggcgac gagcgaggcg cggaatttcg ggagcggatc
       61 gcgggccacg aaggcgtcgg cttcgacggg ttttaggtag ttacaggtgt cgtaagcggc
      121 gtgtccgcgg aggcgcagcg tatgcgcttc gatgagaatc ggacgcgaag tggtgcggac
      181 cttttcgatg gcggcggcga acgtgcgggc ggtgccgacg acatccgtgg tgcaatcgac
      241 ggcgaagcct tccatgccgt agccggcggc gcgacgccag agttcggtgc cttcgcagaa
      301 ctgttcgcag gtcggcgtgg agtaggcgta gcagttgttc tcgatgatga agagaatcgg
      361 gagcgagagg agcgaggcga ggttgagcga ttcgtgaatg tcgccggtgc tcgaaccgcc
      421 gtcgccgaag agggcgaagc cgaccgcggg gcggccgagg cgccgctgcg aatcggcgcc
      481 gccgaggacg gtgctggtca tcgcaccgag gtggctgatc atcgggaacg agcggttttt
      541 cggatcgccg cgatggacgt tgccttcgcg cgcgcgggtg gggctgccgg cgttggcgaa
      601 gtattggttg agatgatcgc agaggtgacc gctccagatg agatgaccgc cgaagtcgcg
      661 atgggtgaag ctgacgacat cgatggcttt gtcggcgagg agcgtgagcg ggacgatgag
      721 gccttcgttg ccctgaccgc cggcgagggt gcctttcatc aggccctgac gaaacaggtc
      781 gaggttgcga ttatcgccgg tgcgagcgag ttgcatccac gtgtagaggc ggagcaaggt
      841 atcgcgggaa ggattttcga gttccgcagc gcggagatcg cgcgccgtga ggatcgccgg
      901 aggagtggga aaagccatgt ccaaagcctc accgtgaggg tggggcgcgg cgcgggcaag
      961 cgagagattt cgcggggagc acagtggggg attttgtcgc ggagaacacg gcgcagatgg
     1021 gtgttcgcag cgggcgtgag aggcgggggc gctagcccga ataaatcgca cgcgaatcaa
     1081 tcaagcgcag gtgcggtcgc ggtggctggt tacacagaga tcacagagtg ccgacacaga
     1141 ggaagcagga ggccacaagg tgttgggagg actttcatgc agatggatgc tgaggacgat
     1201 gacggctcgg
//
LOCUS       DASPLT010001323         1181 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_239784, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001323 DASPLT010000000
VERSION     DASPLT010001323.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1181)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1181)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1181
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_239784"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1181
                     /locus_tag="VEA63_13530"
     CDS             <1..>1181
                     /locus_tag="VEA63_13530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD85078.1"
                     /translation="TPARHLFIGLAVALFLTLTFAMTAQETPSSPENTPPPAAPEAPA
                     TPEAGEPEDGELRRLDVPTPAVVNDQAVERSDDAQATGDNASTSAADAQRNRQKTRFD
                     RRHTGERFNLWRDSNVAENEAVGVVISVFGSSTSAGSVREAVVSVFGSSTSSGYVGEA
                     VISVLGSSRMTGGHVGDAVVSVLGNTYVNGRVDGEVVAVLGNVELGPDAEVHGEVVCV
                     GGTVIRDPRAKVLGQVNNVALGANFGGFEWLHAWVRHCLVLGRPLAFAPHLMWAWWIA
                     IGFLALYFVIALLAPRGVEKCSRTLEQRPGYSLLTTLLIILLTPLALVVVAFTVVGMP
                     VLVLALFAATVFGKVVMLDWIGRRLTGLFGGPNFAPAGAVLVGGVLVLLLYTIPIVGF
                     L"
BASE COUNT          181 a          327 c          429 g          244 t
ORIGIN      
        1 aactccagct cgccatctct tcatcggcct ggcggtcgcg ttgttcctga cgctgacatt
       61 tgcgatgacg gcgcaagaga cgccgtcgtc accggaaaac actccgccgc cagccgcacc
      121 cgaagcgcca gcgacgccgg aagctggcga accggaggac ggcgaacttc gtcgtctcga
      181 tgtgccgacg cccgccgtgg tgaacgacca agcggtcgaa cgttcagacg acgcgcaagc
      241 aaccggggac aatgcgtcga cgtctgctgc cgacgcgcag cggaaccgtc agaaaacgcg
      301 tttcgaccgg cggcacaccg gcgagcgttt caatctttgg agggattcca acgtcgccga
      361 gaacgaagcg gtgggtgtcg tcatttcggt ctttggttcg tccacatcgg ctggatcggt
      421 gcgggaggcg gtcgtttcgg tgttcggctc gagcacatcg agcggctacg tcggcgaagc
      481 ggtgatctcc gtgctgggca gttcgcggat gacgggcggc catgtcgggg acgcggtggt
      541 gtcggtgttg ggcaacacgt atgtgaacgg gcgcgttgac ggcgaagtcg tggcggtgct
      601 cggcaatgtc gagctcgggc cggacgcgga ggtgcacggt gaagtcgtgt gcgtaggggg
      661 aacggtgata cgcgacccgc gtgcgaaggt gctcgggcag gtcaacaacg tcgcgttggg
      721 ggcaaacttt ggcgggttcg agtggctgca tgcgtgggtg cggcactgcc tggtgctggg
      781 gcggccgctg gcgtttgcgc cgcacttgat gtgggcgtgg tggatcgcga tcggatttct
      841 cgcgctctac ttcgtgatcg cgttgctcgc gccacggggg gtcgagaaat gttctcgcac
      901 gctcgagcaa cggccgggct actcgctgtt aaccacgctg ctaatcatat tgctcacgcc
      961 gctcgcgctt gtcgtggtcg cattcaccgt ggttggcatg ccggtgttgg tgctagcatt
     1021 gttcgcggct acggtgttcg gcaaggtcgt aatgctcgat tggatcgggc gacgactgac
     1081 gggattgttt ggcggaccaa atttcgcgcc ggcgggtgcg gtgttggtgg gcggcgtgct
     1141 ggtgctgctg ctctacacga ttccgatcgt cggcttcctt g
//
LOCUS       DASPLT010001324         1697 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_200694, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001324 DASPLT010000000
VERSION     DASPLT010001324.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1697)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1697)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1697
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_200694"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..702)
                     /locus_tag="VEA63_13535"
     CDS             complement(<1..702)
                     /locus_tag="VEA63_13535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RND transporter"
                     /protein_id="HYD85079.1"
                     /translation="MDIPRPDQSKAKRKKRIIYGGIAALVLIGITVGLARLKPAAPTV
                     ERNLVWIGTVERGQMLRQVRGLGTLIPEEITWIAARTQGRVDKIVLRPGAPVEPDSVI
                     LILTNPDVVRAAADADSQLKAAEAEFANLKVQIESSVLAAESVAATAKADYEQAKLRA
                     EVNDELIKDGLVSPLDARLSKVTAEQAATRNSIEQKRYAFTKDSVAPQLAVKAAEVER
                     LRSQAKLRQDELDALT"
     gene            complement(1158..>1697)
                     /locus_tag="VEA63_13540"
     CDS             complement(1158..>1697)
                     /locus_tag="VEA63_13540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HYD85080.1"
                     /translation="DDMRSGLEIIESRAEGLSRFMQAYARLARLPAPSVAPCEIAPLV
                     QRVAALETRRNVEVLPGPALTLSCDCAQIEQVLINLVKNAVDAVRETATAERPDAGVR
                     IAWHKTSGQIEIAVEDDGPGISNSGNLFVPFFTTKPEGSGIGLVLCRQIAENHGGSLT
                     LENRADGQTGSIARLRLPA"
BASE COUNT          269 a          410 c          678 g          340 t
ORIGIN      
        1 ggtgagggcg tcgagctcgt cctggcggag tttcgcttgg gagcggaggc gttcgacctc
       61 ggcggccttg acggcgagct ggggagcgac ggagtccttg gtgaaggcgt agcgcttttg
      121 ttcgatgctg ttgcgggtcg ccgcctgctc ggcggtgacc ttggagagcc gggcatcgag
      181 cggcgagacg aggccgtctt tgatgagttc gtcgttcacc tcagcacgga gcttcgcctg
      241 ttcgtagtcg gctttggcgg tggcggcgac ggattcggcg gcgaggacgc tgctctcgat
      301 ctggactttg aggttggcga attcggcttc ggcggctttg agctgcgagt cggcgtcggc
      361 ggcggcgcgg acaacatcgg ggttggtgag aatgaggatg acgctgtcgg gttcgacggg
      421 cgcgccgggg cggagaacga ttttgtcgac gcggccctgc gtgcgcgcgg cgatccatgt
      481 gatctcctcg ggaatgaggg tgccgagccc gcgcacttgg cggagcatct ggccgcgttc
      541 gacggtgccg atccagacaa gattgcgctc gacggtgggg gcggcgggtt tgaggcgcgc
      601 gagtccgacg gtgatgccga tgagcaccag tgcggcgatg ccgccgtaga tgatgcgctt
      661 cttacgtttg gctttggatt ggtcggggcg cggaatgtcc atgcgtgagg aggtatttgc
      721 aggcgctgtg ccagcggatc tattgatggg taagtgactg atttacaaac ggaagaaatt
      781 tgtgcgaggg aaggacagac ctggtggagc atcgaagccg ggacgggggt gtcccgaaag
      841 tgggatgggg tcgtgtgatg cgaggccgaa gtttagctag gatcgatcac accgaaccga
      901 ccaaggcatg tgacacggag agcacggagc accgacacgg aggaagcagg aggccacaag
      961 gtgttcggag gtgtttcatg cagacgaggg ctgacgacga tgaaggctgg ggtttgaaac
     1021 aactatttcg ctcggtcggc tccgggtcgg agttccgggt tctgagtgcg ctctgcgtct
     1081 cgtttgttga ggtgtgagct gtgcgggttt gggaggtagg ggcgcttgtc ggggcgtaaa
     1141 gcccctccca cagttggtca ggcggggagg cggaggcggg cgatgctgcc ggtttggccg
     1201 tcggcgcggt tttcgagggt gagcgagccg ccgtgatttt cggcgatttg tcggcagagg
     1261 acgaggccga tgccggagcc ttcgggcttg gtggtgaaaa acggaacgaa aagatttccg
     1321 gagttcgaga tgccggggcc atcgtcttcg actgcgatct cgatttgacc ggaggttttg
     1381 tgccatgcga tgcgcacgcc ggcgtcgggg cgctcggcgg tggcggtttc gcggacggcg
     1441 tcgacggcgt ttttaaccag gttgatcaag acctgctcga tctgtgcgca gtcgcacgag
     1501 agcgtgagcg cggggccggg gaggacttcg acgttgcggc gggtttcgag cgcggcgacg
     1561 cgttggacga gcggtgcgat ctcgcacggc gcgacggacg gcgcggggag gcgggcgaga
     1621 cgcgcgtagg cttgcatgaa acggctgagg ccttcggcgc gcgactcgat gatttcgaga
     1681 ccggagcgca tgtcgtc
//
LOCUS       DASPLT010001325         1691 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_261303, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001325 DASPLT010000000
VERSION     DASPLT010001325.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1691)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1691)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1691
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_261303"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..838)
                     /locus_tag="VEA63_13545"
     CDS             complement(<1..838)
                     /locus_tag="VEA63_13545"
                     /inference="COORDINATES: protein motif:HMM:NF012457.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HYD85081.1"
                     /translation="MTFPKSFTWGAAAAAYQIEGAWNEDGKGPSIWDVFSQQPGKVFE
                     NHNGNVACDHYHRWREDVALMAEFELQAYRLSLAWTRIQPTGTGKPNAKGLAFYDRLV
                     DALLAANITPWITLFHWDLPQALQQRGGFLNRDFVEWFGDYAAIVADRLGDRVKHWMT
                     FNEPPCSIGLGLQEGMFAPGLKLPFSDCLLGAHNLLMAHGRGVQALRAGCRGKVKISV
                     AQTSRERIPATETSRDIEAARQDYFGATSRTMWNLAWWADPIVFGAYPEECVKAFGAD
                     MPR"
     gene            complement(873..>1691)
                     /locus_tag="VEA63_13550"
     CDS             complement(873..>1691)
                     /locus_tag="VEA63_13550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007363232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylxylan esterase"
                     /protein_id="HYD85082.1"
                     /translation="DVYHVSFRTIGPSWARTPARIFGILCEPRAPGKYPAVLKVPGAG
                     VRPYSGDKDTAAKGAITLEIGIHGIPVNLPQQVYDVLAAGALNGYWMFNADNRETYYY
                     HRVYLSCVRANDFIASREKWNGKGLVVMGGSQGGQLSIATAALDSRVTGLAAIHPAGC
                     DFAAELHDRAGGWPHPFKTDYEAGKPSEHATPAKIEALRYYDVVNFARRLKMPGYYTW
                     GYNDQTCPPTSVYAAYNVIAAPKTLGVTLEMSHSYNAEQGAAIQGWIDNFLRLR"
BASE COUNT          321 a          605 c          459 g          306 t
ORIGIN      
        1 cccgcggcat gtcggcgccg aacgccttca cgcattcctc cggatacgcg ccgaacacga
       61 tcggatcggc ccaccatgcg agattccaca tcgtgcggct ggtcgctccg aaataatcct
      121 gccgcgccgc ttcgatatca cgcgacgtct ccgtcgcagg gatacgctcg cgactcgttt
      181 gcgcgaccga gatcttcacc ttgccgcgac accccgcgcg caacgcctgc accccgcggc
      241 cgtgcgccat caatagatta tgcgcaccga gcaggcagtc ggaaaacggc agcttcaagc
      301 ccggcgcgaa catcccttcc tgcaaaccca gcccgatcga acacggcggc tcgttgaacg
      361 tcatccagtg cttcacgcgg tcgcccaacc ggtcggccac gatggccgcg tagtcaccga
      421 accactcgac aaaatcgcga ttcaaaaacc cgccacgctg ctgcagcgcc tgcggcaggt
      481 cccaatgaaa cagcgtgatc cagggcgtga tgttcgccgc gagcaacgca tcgaccagcc
      541 gatcgtagaa cgcgaggccc ttcgcattcg gcttccccgt ccccgtcggc tggatacgcg
      601 tccacgcgag cgaaagacga tacgcctgca actcgaactc ggccatcagc gccacgtcct
      661 cgcgccagcg atgatagtga tcgcacgcga cgttgccgtt gtgattctcg aaaacctttc
      721 cgggttgttg cgaaaacacg tcccaaatcg acggcccttt gccatcctcg ttccacgctc
      781 cctcgatttg ataagccgcg gccgcagcgc cccaggtgaa gcttttcgga aatgtcatga
      841 tgcgtgcttg tcgtaattcg cggggaatcg gctcaccgca acctcaggaa gttgtcgatc
      901 cacccctgaa tcgccgcgcc ttgctccgca ttgtagctgt gcgacatctc cagcgtcacc
      961 ccgagtgtct tcggcgcagc gatgacgttg taggcggcgt agaccgacgt cggcggacac
     1021 gtctggtcat tgtaacccca cgtgtagtag ccgggcattt tcaaccggcg cgcgaagttc
     1081 accacgtcgt aataacgcag cgcctcgatc ttcgccggcg tcgcatgctc cgaaggtttg
     1141 cccgcctcgt aatccgtctt gaagggatgc ggccacccgc ccgcgcggtc gtgcaactcc
     1201 gccgcaaaat cgcagcccgc cggatgaatc gccgcaaggc cggtcacgcg cgaatcgagc
     1261 gccgccgtcg cgatcgaaag ctggccgccc tggctcccgc ccatcacgac gagccccttt
     1321 ccgttccact tctcgcgcga cgcgataaag tcattcgcgc gcacgcagct cagatagacg
     1381 cggtgatagt aataggtctc gcgattatcc gcgttgaaca tccagtagcc gttcaacgcg
     1441 cccgccgcca acacatcgta gacctgctgc ggcaggttca ccggaattcc atggatgccg
     1501 atctccagcg tgatcgctcc cttcgccgcc gtgtccttgt cccctgaata cggccgcacg
     1561 cccgcacccg gcaccttcag caccgcagga tattttcccg gcgccctcgg ctcacagagg
     1621 atgccgaaga tgcgcgccgg cgttcgcgcc cagctcggcc cgatcgtgcg gaaactcacg
     1681 tgatatacgt c
//
LOCUS       DASPLT010001326         1503 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_239909, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001326 DASPLT010000000
VERSION     DASPLT010001326.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1503)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1503)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1503
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_239909"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..730)
                     /locus_tag="VEA63_13555"
     CDS             complement(<1..730)
                     /locus_tag="VEA63_13555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007359417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HYD85083.1"
                     /translation="MTHRELHTDFLVVGGGLAGVCAALAAARNGRRVVLVQDRSVLGG
                     NASSEIRMHTVGADIHGRRPGARESGLIEELRLEDAARNPHRSYSQWDLLLYEKVLAE
                     PNLTLLLDTDCTGCAVETLPNGLRRIVSVEAVRQSTEDTFTIHADVFADCSGDGRLGV
                     EAGADFTVGREAKSDYGESLALEVADQQTLGSSILFTARRHATPQPFRAPSWIRRFQK
                     HEFKFRPIEGYEYGYWWAEWGGQLD"
     gene            complement(1101..>1503)
                     /locus_tag="VEA63_13560"
     CDS             complement(1101..>1503)
                     /locus_tag="VEA63_13560"
                     /inference="COORDINATES: protein motif:HMM:NF024863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HYD85084.1"
                     /translation="AHFAAHPADIVLLHSGHNHSVEEDPVPGIVPATERLIAAFRSVN
                     PEVTVLLAQVIPAGKLPKYAYLPELNRALATLAARLHTPAQPVVLIDHAEGFDWTTDT
                     VADKVHPNAAGAEKMAATWFAALKPVLPPPR"
BASE COUNT          286 a          415 c          546 g          256 t
ORIGIN      
        1 tgtcgagctg cccgccccat tcggcccacc agtagccgta ttcgtagcct tcgatggggc
       61 ggaacttgaa ctcgtgtttt tgaaaacgac gaatccatga cggcgcgcga aacggctgcg
      121 gcgtagcgtg gcggcgcgcg gtgaaaagga tcgagctgcc gagggtctgc tggtcggcga
      181 cctcgagcgc gagcgattcg ccgtaatcag acttcgcttc gcggccgacg gtgaaatcgg
      241 cgccggcttc gacgccgagg cggccgtcgc cggaacaatc ggcaaacacg tcggcgtgaa
      301 tcgtgaaggt gtcttcggtc gactgtcgaa cggcttcgac cgacacgatg cgacggagcc
      361 cgttgggcag ggtctcgacg gcgcagccgg tgcaatcggt gtcgagcagg agcgtcagat
      421 tgggttcggc gagaaccttc tcgtagagca gcagatccca ttgcgagtag gagcggtggg
      481 ggttgcgggc ggcatcctcg aggcggagtt cctcgatgag gccggactcg cgcgcaccgg
      541 ggcggcggcc gtggatgtcg gcgccgacgg tgtgcatgcg aatctcgctg gaggcattgc
      601 cgccgagcac ggagcggtcc tggacgagga cgacgcggcg gccgttgcgc gcggcagcaa
      661 gcgcggcgca cacgccggcg agtccgccgc cgacgacaag aaaatcggta tgaagctcgc
      721 ggtgagtcat cgcgggaaaa aagaaacaag cgcgcggacg cggagcgtgg gattcggcgc
      781 acggcgaagc ggccgtgaaa agggggacat gacgcgactg ttcgcgcggt gtgggaccgg
      841 cgcgaatcgc ggcgcactga aatcgctcag tgagaccgtg gcgcgagtgg aatagacatc
      901 accgaaatcg gaatgcagtt aaccgctaat catcgcttat ttccgctaat cgaattccgc
      961 gaaccgacga aatgctcgcg cttcgccgat taggtgaagc ccgcactcgt ccgcatgcat
     1021 caacgttttt gaaaaatcag cgtagattag cggttcaaga actctaacgt tccggttaaa
     1081 cttggcccga cccaagggct ctagcgtggc ggcggcagga cgggtttcaa tgcggcgaac
     1141 caggtggcgg ccatcttctc ggcgccggcg gcgttggggt ggactttgtc ggcgaccgtg
     1201 tcggtggtcc aatcgaagcc ttcggcgtga tcgatcagca cgacgggttg cgcaggcgtg
     1261 tggagtcgcg cggcgagggt cgcgagcgcg cgattcaatt cgggcagata agcgtatttc
     1321 ggcagtttgc cggcggggat gacttgcgcg agcaacacgg tcacctccgg gttgacggaa
     1381 cggaaggcgg cgatgagtcg ctcggtcgcg gggacgatgc ccggcacggg atcttcctcg
     1441 acgctgtgat tgtgaccgct gtgcaggagc acgatatcgg cgggatgggc ggcgaaatgc
     1501 gcg
//
LOCUS       DASPLT010001327         1125 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_16372, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001327 DASPLT010000000
VERSION     DASPLT010001327.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1125)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1125)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1125
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_16372"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(284..>1125)
                     /locus_tag="VEA63_13565"
     CDS             complement(284..>1125)
                     /locus_tag="VEA63_13565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009511587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="exosortase/archaeosortase family protein"
                     /protein_id="HYD85085.1"
                     /translation="FVAFAIYDRWPRIIAGVGACAAENSARVSGVGRWVLNVGVGVML
                     TGGVLLFLLGAFYRAGAGTSQPGTLALTLGAICIVLPLLFLNTPAAAAPRAAGLFDDA
                     RVRLTALFIFPTLVWLVSAPMVSVIESQLNLFLLRKVVTVVSFVFDVLGLPIEQQGNV
                     LVLPTGSVGVEDACSGIRSLTACLFAGSFLAAVFLDRLWKKVSLVAAAMGFAFLTNLL
                     RGLFLTGWAYRNGPDAIEGTVHDIAGYAVLGLTVVGLLCLLPLFNLRFADDAEPAEAN
                     TGN"
BASE COUNT          265 a          432 c          276 g          152 t
ORIGIN      
        1 cccgccatgc gcgccgaaac gctgcgcaaa attctcgcga taaccgccgc gtcggcgcat
       61 gcgccacaca tagcgcggcg ccatcacgat gagcgcgggc agaaaaagca gtcgataaag
      121 ccagagcatg aagggaagcg cgcccactca aaactccgcc gccgcttttc ccagcctttt
      181 tccgaccaca cgccaccgcc atcttcggaa tcctcgcgtg ttctccgatc ctcccacgct
      241 caagagggga ctcacgccac ctcactcacc attcgttgtg ggtttaattt cccgtattcg
      301 cctctgccgg ctccgcgtcg tccgcgaacc gcagattgaa taacggcagc aagcacagca
      361 ggccgacaac cgtcagcccc agcaccgcgt agcccgcgat atcgtgcacc gtgccctcga
      421 tcgcatccgg cccgttgcga tacgcccagc cggtgagaaa caacccgcgc agcaggttgg
      481 tgagaaacgc gaatcccatc gccgccgcga ccagcgagac ttttttccac aatcgatcga
      541 gaaacaccgc cgcgagaaac gatcccgcga acaagcacgc cgtcagcgaa cgaatccccg
      601 agcacgcgtc ttcgacgccc acacttcccg tcggcagcac cagcacgttt ccctgctgct
      661 cgatcggcag cccgagcacg tcgaacacga acgacaccac cgtcaccacc ttgcgcagga
      721 gaaacaaatt caactgcgat tcgatcaccg acaccatcgg cgccgacacc agccacacca
      781 gcgtcggaaa aataaacagc gccgtcagcc gcacgcgtgc gtcgtcgaat aatcccgccg
      841 cccgcggcgc cgccgcggcc ggcgtattca agaacagcaa cggcagcacg atgcagatcg
      901 cacccagcgt cagcgctagc gtgcccggct gcgacgtccc ggcgcccgcg cgataaaacg
      961 ctcccagcaa aaacaacagc acgccacccg tcagcatcac gccaacgccg acattcagca
     1021 cccatcgtcc aacccccgaa acgcgcgcac tgttttccgc cgcgcacgca ccgacgcccg
     1081 cgatgattcg cggccagcga tcataaatcg caaacgccac aaacg
//
LOCUS       DASPLT010001328         1134 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_39820, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001328 DASPLT010000000
VERSION     DASPLT010001328.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1134)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1134)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1134
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_39820"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            154..894
                     /locus_tag="VEA63_13570"
     CDS             154..894
                     /locus_tag="VEA63_13570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HYD85086.1"
                     /translation="MAGHNKWSKVKRLKAVTDSRRGKVFSRLSRDITLAAKSGGGDPA
                     GNARLRTLLLKARDANMPVDNIDRAIKKGTGELPGVVFEEMTYEGYGAGGVAFVVQVT
                     TDNKTRAAKDVRSVFSRHGGNLASTGAVSFQFLHAGQFLVSKEQTTEDALLETALEAG
                     ADDVIASEQGFEVRCSIQNFDKVSHALEQKGLKPDSAEIAYIPTTTVPVSDPHVAQTL
                     AKLHDALEELDDVQNVFSNEEVADSVAT"
BASE COUNT          240 a          411 c          298 g          185 t
ORIGIN      
        1 ttccagcgcc cgacgaagaa cgtcaccccg aagaagaaaa agtaagcggt atgtagggcg
       61 gggtctcccg cccccgccgc cgggcgtcgc tcgcattttc ccaactcgaa acccggaact
      121 caaaactccg aaccgaaaag cgaagatttt tccatggctg gccataacaa gtggtccaag
      181 gtcaaacgcc tcaaagccgt caccgactca cggcgcggca aggtcttctc ccgtctctcg
      241 cgcgatatca cgctcgccgc caaatccggc ggcggcgatc ccgccggcaa cgcccgcctc
      301 cgcacgctcc tgctcaaggc gcgcgacgcc aacatgcccg tcgacaacat cgaccgcgcg
      361 atcaagaagg gcaccggcga actccccggc gtcgtcttcg aagaaatgac ctacgagggc
      421 tacggcgccg gcggcgtcgc cttcgtcgtg caagtcacga ccgacaacaa gacgcgcgcg
      481 gcgaaggacg ttcgctcggt cttctctcgc cacggcggga atctcgccag caccggcgcc
      541 gtgtcgttcc agtttctcca cgccggtcag ttcctcgtct cgaaagaaca aaccaccgaa
      601 gacgccctcc tcgaaaccgc gcttgaagcc ggcgccgacg acgtgatcgc cagcgagcag
      661 ggcttcgaag tccgctgcag tatccaaaat ttcgataagg tgtctcacgc gctcgaacaa
      721 aaaggcctca aacccgacag cgccgaaatc gcctacattc cgacgaccac cgttcccgtc
      781 agcgatcctc acgtcgccca aaccctcgcg aaactccacg acgcgctcga ggagctcgac
      841 gatgtgcaaa acgtcttctc caacgaagaa gtcgccgaca gtgtcgccac gtagcgcagg
      901 caacctgccc gcgttatctt atcttctccg ctcaattcgc cgcgctgaag ggtgcgcggc
      961 ccggccgcga tgacatacgc cgcccttgtc tccattccgt ggatgcgcga ggttgcgccc
     1021 ttcgcatgac cgacgacacg accaacgttc tcgttcgccc gccgcgcgcc gccggcgagg
     1081 tcggcatcgt cgtgcctcac gatttcgttt acgcgaaacc cttcacgttc aaaa
//
LOCUS       DASPLT010001329         1673 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_39835, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001329 DASPLT010000000
VERSION     DASPLT010001329.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1673)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1673)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1673
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_39835"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..978
                     /locus_tag="VEA63_13575"
     CDS             <1..978
                     /locus_tag="VEA63_13575"
                     /inference="COORDINATES: protein motif:HMM:NF024353.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter-associated beta strand
                     repeat-containing protein"
                     /protein_id="HYD85087.1"
                     /translation="ISGPISNGTSGGSLGITKIDSGTLTLKGVDSFTGAIAVEGGTVE
                     LTANNVLRAYQPANGGVGNAVSVSTDATFAVSGGNNYTNTIGSVTGTGDVSIAASTIL
                     KVANGSDNTFDGRLTGEGLFEKTGAATFTFSSTANTSEFSFDGTVQVTNGTMEFQGGT
                     ALDALFIGELKTLANTTLFLNESFINVGTLNITGNTILDFGTGDPSILNADNIYIAEG
                     AVLTIRNWSSEVDFLFANVAFRQIDGGGTLAGFNTDGTAPQNQVHFEGDPLSPDGSHT
                     GWINYDYQGYTNWEIRPIPEPATYGAMLLGGCVALLGWRRYTTRRKRAG"
     gene            1009..>1673
                     /locus_tag="VEA63_13580"
     CDS             1009..>1673
                     /locus_tag="VEA63_13580"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HYD85088.1"
                     /translation="MRDTLTSVPVDSSISSSSTAPVSTPTPHVLVVEDETKTRASIAE
                     GLELERWRVSTADSGSGALLLLEAQAFDLVVLDWMLPDRDGLTVLRHIRQRGNQVPVL
                     ILTARSTVADRVNGLESGADDYLPKPFAFAELVARSRALLRRPILSTGQFLRCGDLQL
                     DTRARVAVRGGQPISLTPREVEILEYLLRYQGQIVSREMLERDVWKQSHRFTSLDNVI
                     DVQ"
BASE COUNT          320 a          603 c          470 g          280 t
ORIGIN      
        1 atcagcggcc ccatttccaa cggcacgagc ggcggttcgc tcggcatcac gaagatcgat
       61 tcgggcaccc tcacgctgaa gggcgtcgac agtttcaccg gcgcgatcgc cgtcgaaggc
      121 ggcaccgttg agctcacggc gaacaacgtc cttcgcgcct accagcccgc gaacggcggc
      181 gtcggcaacg cggtcagcgt ttcgaccgat gcgaccttcg ccgtttccgg cggtaacaat
      241 tataccaata cgatcggcag cgtgaccggc accggcgacg tcagcatcgc cgccagcacg
      301 attctcaaag tcgccaacgg ttccgacaac acgttcgacg gccggctcac cggcgaaggc
      361 ctcttcgaaa aaaccggcgc cgccaccttc acgttttcga gcacggcgaa caccagcgag
      421 ttctccttcg acggcaccgt gcaggtcacc aatggcacga tggagttcca gggcggcacg
      481 gcgctcgacg cgctgttcat cggcgaactc aagacgctcg ccaacaccac gctcttcctg
      541 aacgagtcgt tcatcaacgt cggcacgctc aacatcaccg gcaatacgat cctcgacttc
      601 ggcaccggcg atccctcgat cctcaacgcc gacaacatct acatcgccga gggcgccgtc
      661 ctgacgattc gcaactggtc gagcgaagtg gatttccttt tcgccaacgt cgcgtttcgc
      721 cagatcgacg gcggcggcac gctcgccgga ttcaacaccg acggcaccgc gccccagaac
      781 caggttcact tcgaaggcga cccgctttca ccggacggca gccatacggg ctggatcaac
      841 tacgattacc aaggctacac caactgggaa atccgcccga tccccgaacc ggcgacctac
      901 ggcgcgatgc tcctcggcgg ctgcgtcgcg ctgctcggct ggcggcgcta cacgacgcga
      961 cggaagcgcg cgggttgacc gcacgacgaa gaacttgaag gcgcggaaat gcgcgacacg
     1021 ttgacgagcg tgcccgtgga ctcgtcaatt tcttcctcct ccaccgctcc cgtttcgacg
     1081 ccgacacctc acgtcctcgt cgtcgaggac gaaacgaaaa cccgcgcctc gatcgccgaa
     1141 ggcctcgagc ttgaacgttg gcgggtcagc accgccgatt ccggcagcgg cgccctcctc
     1201 ctgctcgaag cgcaggcgtt cgatctcgtg gtgctcgact ggatgttgcc cgatcgcgac
     1261 gggctcaccg tgctgcgcca cattcgccag cgcggcaatc aggtcccggt gctcattctc
     1321 accgcgcgca gcacggtcgc cgaccgcgtc aacggcctcg aaagcggcgc cgacgattat
     1381 ctgccgaaac ccttcgcatt cgccgaactc gtcgcccgca gccgtgcgtt gttgcgccgc
     1441 cccattctga gcaccggcca gttcctgcgc tgcggcgatc tgcaactcga cacccgcgcg
     1501 cgcgtcgccg ttcgcggcgg ccagccgatt tcactcacgc cgcgcgaggt cgagattctc
     1561 gaatacctgc tccgttacca gggtcagatc gtgtcgcgcg aaatgctcga acgcgacgtc
     1621 tggaaacaaa gccaccgctt cacctcgctc gacaacgtga tcgacgttca aat
//
LOCUS       DASPLT010001330         1530 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_220191, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001330 DASPLT010000000
VERSION     DASPLT010001330.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1530)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1530)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1530
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_220191"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1530
                     /locus_tag="VEA63_13585"
     CDS             <1..>1530
                     /locus_tag="VEA63_13585"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HYD85089.1"
                     /translation="FSVWHGESEFIPLLEPRNFAPTATDSFYAPGFAFWYMFGGMHGS
                     AEAAASPRAIAPGPDHPLLRNLETLDQIYRTPDPQKRIALFQQIAERNAENVWTISIG
                     TPPPQLVVVKNGFRNVPRTAVWQGTFESPGNAGLETYFWDEPRDPPGVLADLKAAMIA
                     PAALTSAAPGASTPAASATATRGAGAVAGALLKWALIAIAVVGVILVACRHPFIARRL
                     MVVLPTLGVISVVTFAIVQLPPGDFATTRALEYESQGTPDGAHTVRELRATFHLDESV
                     VKQYFRWVGLLWFTTFDAGDKGLLQGDLGRSMEFDRPVNAIVGDRITLTIVVSLATIL
                     LTWVIALPIGIYSAVRQYSFGDYVLTFAGFLGMSVPGFLLALVLMYFSHRFFGLSPSG
                     LFSPEFATTSGWSWPKFIDLLKHLWLPTVVLGLGGTAGMIRVMRANLLDELRKPYVVT
                     ARAKGVSELKLLLKYPVRLALNPFISGIGAFFPQLISSGAIVAIVLSLPLVGPVMLTA
                     LL"
BASE COUNT          232 a          486 c          492 g          320 t
ORIGIN      
        1 ttcagcgtgt ggcacggcga gagcgaattt atcccgctgc tggagccgcg taacttcgcg
       61 ccaacggcca cggactcgtt ttatgcgcct ggcttcgcgt tctggtatat gttcggcgga
      121 atgcacggca gcgccgaagc cgcggcttca ccccgcgcga tcgcaccggg accggaccac
      181 ccgctgctgc gcaacctcga gacgctcgat caaatttatc gcacgccgga tccgcagaag
      241 cgtatcgcgc tgtttcagca aatcgcggag cgcaacgcgg agaatgtctg gaccatcagc
      301 atcggcacgc cgccgccgca actcgtggtg gtgaaaaacg gatttcgcaa tgtcccgcgc
      361 accgcggtgt ggcaggggac gttcgagagc ccgggcaacg ccggccttga aacgtatttc
      421 tgggacgagc cgcgcgatcc accgggcgtg ctcgcagacc tgaaggcggc aatgattgcc
      481 ccggcggcgc tgacgagcgc cgcgccgggg gcatcgacgc cggcggcgag cgccactgcg
      541 acacgcggcg ctggcgcggt cgcgggagca ttactgaagt gggcgttgat cgccatcgcg
      601 gtcgttgggg tgattctcgt cgcgtgtcgt catccgttca ttgcgcgacg gctcatggtc
      661 gtgttgccca cgctgggcgt gatttccgtc gtcacgtttg caatcgtcca acttccgccg
      721 ggcgacttcg ccaccacgcg cgcgctcgaa tacgaatcgc agggcacgcc cgacggcgcg
      781 cacaccgtgc gcgaactgcg cgcgacgttc catctcgatg agtcggtggt gaaacagtat
      841 ttccgctggg tcggcctgct gtggttcacg acgttcgacg ccggcgacaa ggggctgctt
      901 caaggcgatc tcggccgctc gatggaattc gaccggccgg tgaacgcgat tgtcggcgat
      961 cgcatcacgc tgacgatcgt ggtgtcgctc gccacgattc tcctgacgtg ggtcatcgcg
     1021 ctgccgatcg gcatttattc cgcggtgcgc cagtattcgt ttggcgacta cgtgctcaca
     1081 ttcgcgggct ttctcgggat gtcggtgcct ggcttcctgc tcgcactcgt gctgatgtat
     1141 ttctcgcacc gctttttcgg gctgagtccg tcgggtctgt tctcgccgga gttcgccacg
     1201 acgagcggct ggtcctggcc caagtttatc gacctattga aacacctttg gctgccgacg
     1261 gtcgtcctcg ggctcggcgg cacggccgga atgatccgcg tgatgcgcgc caacctgctc
     1321 gacgaattgc gcaagcccta cgtcgtgacc gcgcgtgcga aaggcgtgtc ggagttgaag
     1381 ctgttgctga agtatccggt gcgcctcgcg ctcaatccgt tcatctccgg aatcggcgcg
     1441 ttttttccgc agctcatttc cagcggcgcc attgtggcca tcgttctcag cctgccgttg
     1501 gtcggtccgg tgatgctcac cgcgctgttg
//
LOCUS       DASPLT010001331         1811 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_117406, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001331 DASPLT010000000
VERSION     DASPLT010001331.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1811)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1811)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1811
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_117406"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..484
                     /locus_tag="VEA63_13590"
     CDS             <1..484
                     /locus_tag="VEA63_13590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HYD85090.1"
                     /translation="DNYGGEFENRIRFLLETTRAVRVVWPDNLPLTVRLSCTDWMEGG
                     WDIEQTIELARRLKNEGVDLIDCSSGGAVPNAKIEIGPGYQVPFAERVRREAGIATAA
                     VGLITEPKQADEIVRSGKADVVLLAREFLRDPYWPAHAARALGQAKAVPPPKQYARAW
                     "
     gene            complement(553..1119)
                     /locus_tag="VEA63_13595"
     CDS             complement(553..1119)
                     /locus_tag="VEA63_13595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HYD85091.1"
                     /translation="MLLAFHKPYGVLSQFTPEPGSRWRTLAEFQLPKNVYALGRLDAD
                     SEGLLLLSDEPGLNSRLLDPEHGHRREYWAQIERVPDDASLAALERGVKLNDYVTRPC
                     RARLLHPAPSLAPRDPPIRYRKNVPDCWLSLELIEGKNRQVRRMTAAIGHPTLRLVRA
                     RIGDFSLGELVPGTWRELTAAERHTVFR"
BASE COUNT          362 a          472 c          641 g          336 t
ORIGIN      
        1 cgacaattac ggcggtgaat tcgaaaaccg gatacgcttt ctgctcgaaa cgacgcgagc
       61 ggtgcgcgtg gtctggccgg ataatcttcc gctgacggtc cggttgtcgt gcaccgactg
      121 gatggagggc ggctgggaca tcgaacagac gatcgaactc gcgcgacggt tgaagaacga
      181 gggcgtggat ctgatcgatt gcagctcggg tggtgcggtg ccgaacgcga agatcgaaat
      241 cgggcccggt tatcaggtgc cgtttgccga gcgtgtgcgc cgcgaggccg ggatcgcgac
      301 ggcggccgtg ggattgatca cggagccgaa gcaggcggat gaaatcgtgc gcagcggcaa
      361 ggcggacgtc gtgctgcttg cgcgagagtt tttgcgcgat ccgtattggc cggcgcatgc
      421 ggcgcgggcg cttgggcagg cgaaggcggt tcctccgccg aagcagtatg cgcgggcgtg
      481 gtgaagggga ggagtagggc gcggctttag cccgctttcg atggcgccca aaaggcgggc
      541 tgaagcatcg ccctacctaa agacagtgtg gcgttcggcg gcggtgagct cgcgccatgt
      601 tcctggcacg agctcgccga gggagaagtc gccgatgcgc gcgcgcacga gtcgaagcgt
      661 gggatggccg atggcggcgg tcatgcgccg gacttgccgg tttttgcctt cgatcagctc
      721 gagcgagagc cagcagtcgg gcacgttttt gcggtagcga ataggcggat cgcgtggcgc
      781 gagcgacggc gcgggatgga gcaagcgcgc gcggcacgga cgcgtgacgt agtcgttcag
      841 ttttacgccg cgttcgaggg cggcgagcga tgcgtcatcc ggcacccgtt cgatttgcgc
      901 ccagtattcg cggcggtgtc cgtgttcagg atcgaggagc cgcgagttga ggccgggttc
      961 atcgctgagc agcagcaaac cttcggagtc ggcgtcgagt cgaccgaggg cgtagacatt
     1021 tttgggcaat tggaattccg cgagcgtgcg ccagcgcgag ccgggttcgg gcgtgaactg
     1081 cgagaggacg ccgtaaggtt tgtggaaggc gagaagcacg gggaggcggg aagtagcagg
     1141 tgacaagtag caagtaacaa gacgcaggtg aggaaaaacc gcggatgaac aggtagcgac
     1201 gtggattatg gccggagttt ttaaccgcta atctacgctg attttttcgg agctggcggg
     1261 cggatgaagc ggcgcgtgcc tcgcctaatc ggcgaagcgc ggacgaccag atgagttgat
     1321 gtgtatccag acgtggcacc acgagtggac acaattcatg cgaacgaaag gacaggtgcc
     1381 ggcgagacga gccgcagaag taaccatgga tgaacaccga tcgaacctct cgaccattcg
     1441 acctcctcga aatgtgttca tccgcggttg ataattgcga tccgattgaa ccacgaatgg
     1501 acacgaattt acacgaatga cggattcgaa ttcgtttcgc tcgggtatca atcgaatcgc
     1561 cagccctcgg gcggctcagc ttgcggatcg ggactcttgc tacttgatac gtgttacttg
     1621 tcactttcct ccacatgcgc gccgttcgac ttgaagccat caacgaattg aaaattgccg
     1681 aagtgcccgc gcccatcgcg gcggcaggcg aggcggttgt cgacctccgc gcggcggcgc
     1741 tcaatcaccg cgatgtttgg atcaagacgg gacaatacgc gggtttgaaa tatccgagca
     1801 ctccgggctc g
//
LOCUS       DASPLT010001332         1072 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_158740, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001332 DASPLT010000000
VERSION     DASPLT010001332.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1072)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1072)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1072
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_158740"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(302..>1072)
                     /locus_tag="VEA63_13600"
     CDS             complement(302..>1072)
                     /locus_tag="VEA63_13600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD85092.1"
                     /translation="RSADATTNQEQKLARLWIAVRELMAAPFEEARFAEFRPHWNQAL
                     SQWEGAAAWYGLHAHIYLGCRAAIATGARVRAVLREHLPRGAHARDFPFPATGLASAT
                     YSIAKLAPRGPIRKQLFTEALGYLAGDSDDAGRQSDILALRGSILLRTARFSEAVTAY
                     TTCLRIRETLAWPTERIGDSMSELGFACVLTGDVRRGLELQRRGNDLLAHGGIGFQVR
                     AKKKLALTLLATGHLAEAWLLHQEARQLAASHRMFDQL"
BASE COUNT          196 a          308 c          349 g          219 t
ORIGIN      
        1 gtgtttcttc ctacacagat ggcgcgagga aaactcgtgc ttgaaaatgg cgacgtagtg
       61 gcgatccctc cgccagattg gccgagcggg tcggccgaga gcacatctct gataccgccg
      121 atgccgacct ctccaactga cctcccgccg gttacaccga ccggatagtt gtaattgttt
      181 cgcccgatct gatgtcccgt cgccaatata aaccgttacc tgtttcagga acggttctcg
      241 attcccagag gagaaaagcg acggcgtcgt ggcacattga tacgggtcgt cggacaaggc
      301 gctaaagttg gtcgaacatg cgatgagagg cggcgagttg acgtgcttcc tggtgcagta
      361 gccacgcttc cgcgagatgc ccggtcgcga gtagcgtcag agcgagtttc tttttagccc
      421 tgacttggaa tcctattcct ccgtgcgcga ggagatcgtt gccccgtcgc tgcagttcga
      481 gcccccgacg aacgtcaccg gttaagacgc atgcgaaccc gagttcggac atggaatcgc
      541 caattcgctc ggtgggccag gcgagtgttt cccggatccg gaggcatgtg gtgtaggccg
      601 tgacggcttc ggagaagcgg gcggtcctca agagtatgct cccacgcaac gcgaggatgt
      661 cggattggcg tcccgcatcg tcggaatcgc cggcgagata accgagtgct tcggtgaaca
      721 gctgctttcg aatcggcccg cgtggggcaa gtttggcgat cgaatacgtc gcgctggcga
      781 ggcctgtcgc ggggaaggga aagtcgcgcg cgtgggcgcc gcgcggcagg tgttcacgta
      841 agacggcgcg cacccgggcg cccgttgcga tcgctgctcg gcaaccgagg taaatatgcg
      901 cgtggagtcc ataccatgcg gctgcgcctt cccactggct gagcgcttgg ttccaatgtg
      961 ggcgaaattc agcgaagcgc gcttcctcga agggcgcggc catcaactcg cgcacggcga
     1021 tccatagccg tgcgagcttt tgttcctggt tggtcgtggc gtcggcgctt cg
//
LOCUS       DASPLT010001333         1695 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_16525, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001333 DASPLT010000000
VERSION     DASPLT010001333.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1695)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1695)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1695
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_16525"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(302..1285)
                     /locus_tag="VEA63_13605"
     CDS             complement(302..1285)
                     /locus_tag="VEA63_13605"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HYD85093.1"
                     /translation="MNRISLPRTSLAVSPLCLGTAFFGADIDQATSFALLDRFVERGG
                     NFIDTARIYSDWAPGERHRSERLLGDWLETRGHRSSLVIATKGAHPELETPTVPRSSA
                     AEIRTDLEGSLRSLRVDAVDVYWLHRDDPSRPVEHFIDLLNAFLREGLIRSFGASNWT
                     ADRLRAAHDYAQRSGQKGFVANQPLWSLGSRHAHPSPIHGLVRYDPAAHAFHHETGLA
                     VIPYSSQASGFFSKLALPADRRPPGLGKHAFHTPPNIAAGEIVARLAEEKGVAPSAIV
                     LAYLWSRSFPVVPIVGCRTIEQLDDSIRAVPVRLTDEERQALETASDSGLT"
     gene            1502..1663
                     /locus_tag="VEA63_13610"
     CDS             1502..1663
                     /locus_tag="VEA63_13610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD85094.1"
                     /translation="MKRFLASALLLAASANQILALQPDIPVPESGSTAGALVLAGLAL
                     FALRRTLKK"
BASE COUNT          313 a          488 c          569 g          325 t
ORIGIN      
        1 acctgatcgt gcagagcacg gtcggtgtgg gaacgacatt cacgctcacg cttccgctcg
       61 cctcatccga gtgaacggcg atgtgcgctg ccggggagcg actgctcgcg aatcaccgaa
      121 acttcaccag cgttgctggc acggccgggc ctccgaacca cgagatcatg ttggcagagc
      181 tttgggctct tttcggcgaa gacgccgaaa tccgcagcgt gagcatgcgc tgcacgacca
      241 aggtctgtat cgggcggctc tgatccgaac tccgcgacag tcggtttcgc ttaacgtctt
      301 ctcaggtcag gcctgagtct gacgcggttt caagcgcttg ccgttcttcg tcggtcaatc
      361 gaacggggac agcgcgaatg ctgtcgtcga gttgctcgat cgttcggcag ccgacgatgg
      421 gcaccacggg gaacgagcgc gaccagagat acgcgagcac gatggcactg ggcgcgacgc
      481 ctttctcttc ggcgaggcga gctacgatct cgccggccgc gatattgggc ggtgtatgaa
      541 acgcgtgttt ccccaagccg ggcggacgtc ggtcggcagg caaggcgagc ttggaaaaaa
      601 atccgctcgc ttgagacgag taggggatca ccgcgagacc tgtttcgtgg tgaaacgcat
      661 gcgcggcggg atcgtagcgc acgaggccat gaatcggcga cggatgtgcg tgtcgcgagc
      721 cgagcgacca cagcggctgg tttgcgacga aacctttttg acccgagcgc tgcgcgtaat
      781 cgtgtgcggc gcggagacga tcggcggtcc agttcgacgc gccgaatgag cggatcaggc
      841 cttcgcgaag gaacgcgttg aggagatcga tgaagtgctc gactggccgc gacggatcgt
      901 cgcggtgcaa ccaatacacg tcgactgcat cgacacgcag tgagcgcaaa cttccttcga
      961 ggtcggtgcg gatttccgcc gctgacgaac gcggcacggt cggcgtttcc aattcagggt
     1021 gggcgccttt ggtggcgatc acgagcgatg aacgatgccc tcgagtctcc agccagtcgc
     1081 cgagcagacg ctcgctacga tgtcgttcgc ccggtgccca gtcggagtaa atgcgcgcgg
     1141 tgtcgatgaa gttgcctccg cgctccacga atcgatcgag tagcgcgaag cttgtggctt
     1201 gatcgatatc ggcgccgaaa aacgcggtgc caaggcacag gggcgagaca gcgaggctgg
     1261 ttcggggcaa ggagatgcgg ttcatcactc gcgcagcgtg cggatcagtg cgtcgatcag
     1321 tcgaggtgac attcggggcg caggtgagat ggggctgtcg gaatagttga cattcttgag
     1381 taggcgggac gcggctggcg gagccggttg tttcgtaacc cgcagagtta tagatgcttg
     1441 aaccgtgagt cgcggcagtg gcattcatgc tgctcactcg agagcgcaat cccttaatca
     1501 catgaaacgt ttcctcgctt ccgctctgct tctcgccgcg tccgccaacc agatcctggc
     1561 cttgcagccg gatattccgg ttccggaatc cggcagcacc gcgggagcgc tcgtgctcgc
     1621 cgggctcgcg ctcttcgcgc tccgtcgcac gttgaagaag tgattcgcgg tggtccgctc
     1681 gggcggacac gcggt
//
LOCUS       DASPLT010001334         3625 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_220293, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001334 DASPLT010000000
VERSION     DASPLT010001334.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 3625)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3625)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3625
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_220293"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..366)
                     /locus_tag="VEA63_13615"
     CDS             complement(<1..366)
                     /locus_tag="VEA63_13615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HYD85095.1"
                     /translation="MRRFFDTARLLLVCVFGGAVITGCGVRENDIASRVIRVWCHQGQ
                     EAENRAMREIAAAFNATHADRGVRVALTFFPDFQYTEKIAIAAAAGDLPDAFDVDGPL
                     VARYVDAGLLAPLEPFFERA"
     gene            1021..1398
                     /locus_tag="VEA63_13620"
     CDS             1021..1398
                     /locus_tag="VEA63_13620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPW repeat protein"
                     /protein_id="HYD85096.1"
                     /translation="MRFIPTRVHGILDYLVGVLLIFAPRIFGFENTGAASTVPVVLGI
                     AALIYSLLTRYELGLIKVLPFKAHLAMDFASGVVLLVSPWAFGFSETVWVPHVIVGLL
                     EIGAATMTRTVPGDVAPHVPAMR"
     gene            complement(1573..>3625)
                     /locus_tag="VEA63_13625"
     CDS             complement(1573..>3625)
                     /locus_tag="VEA63_13625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FdhF/YdeP family oxidoreductase"
                     /protein_id="HYD85097.1"
                     /translation="IGADFFHQHSVAELAAKSDHWLGHQGRLIEPLVLRPGATHYEPI
                     AWSDAFRLIADELKALGSPDEAVFYTSGRTSNEAAFLWQLFVRAFGTNNLPDCSNMCH
                     ESTSIALPQMIGIGKGCVRLSDFEKADAIFIIGQNPGTNHPRMLTSLQAAKARGCKIV
                     GVNPPPETGTERFKNPQDLLHPTRVPRFFLTGGTTLSDLWLPVLINGDMAFLLGLMKE
                     LLAEEERRPGEVFDHEFIRHHTEGYEQLIAQLRATSWDDIVSFSGLSREQIRAAADIA
                     TASKRIIVCWCMGITQHKNAVATIQQIMNFLLLGGHIGKPGAGPCPVRGHSNVQGDRT
                     MGIWERPKKEFLDKLAAEFRFEPPREHGLDTVDSIRAMNEGRAKVFVAMGGNFLSATS
                     DTELTASGLRNCRLTAHVATKLNRSHVVTGRAALILPCLGRTDIDRQASGEQFVTVED
                     SMGVINPSRGNLEPPSEKLLSESAIVAGLAKAVLGPTYPLDWDAHVANYDRIRDRIEA
                     VVPGFPAFNERIREGTFYLPNPPRDERRFNTPSGKAIFKAAPLTRAAIPEGHYLLTTI
                     RSHDQFNTSIYGLDDRYRGIYNGRRVIFMNEDDLRAAGFVQGQLVDITSHFEGEQRHV
                     EQFMVAPYNIPRRCTAAYFPEANALVPNRSTADESNTPASKSVVITLRASASQIPSRS
                     T"
BASE COUNT          659 a          981 c         1255 g          730 t
ORIGIN      
        1 ggcgcgctcg aagaacggct cgagcggcgc gagcaaaccg gcgtcgacgt agcgtgcgac
       61 gagcggacca tcgacatcga acgcgtcggg caaatcgccc gcggcggcgg cgatggcgat
      121 tttctcggtg tattgaaaat cggggaagaa cgtgagcgca acgcgcacgc cgcgatccgc
      181 gtgagtggcg ttgaacgcgg cggcgatctc gcgcatcgcg cggttctcag cctcttgccc
      241 ctgatggcac cacacgcgga tgacgcgaga ggcgatgtcg ttttcgcgca cgccacagcc
      301 ggtgatcacg gcgccgccga acacgcagac aagaagcaaa cgtgcggtgt cgaaaaatcg
      361 gcgcatggga aaaagcgagc gtgaggacta catcgtgcgc gaggacgtgc gacttgcgcc
      421 caagcagaaa gacgggggcc gccggaaaac gttcgcaagc ggtttttcca gatcgtgaag
      481 aacgaaacca gtttcgccaa ccgcccgtag ccggatccat gcattcagat gtcgcatatg
      541 cgacatataa gtgcacgggt gcgctttgtg cgccggagcc attcgctgtg gtctagccac
      601 tatgcatcgg atcccgtttg ggttgtcgcg ggcgcccggg cacttgatga accgcacaat
      661 cgaaggaatg gaacgcattt gtagccggtg agctcggcga gcggcaggcg cgagcctctc
      721 gaaaacgtcg cgatgcttgt gtgcagttat gtgtcgcata tgcgacacgc caaagtttgg
      781 acgaggacac ggcagcgcgg ggcgtgggct tgtagcgtgc gggactttgc gggggttgac
      841 cgatgttcct cctgggactt tgtgcgatgc gaccggttag gaccgctcgc ctgtggctgc
      901 agggagagtt ccccctttcg attgcacgga gcgtaggcta gattgcgcca acacgttcac
      961 ggcgccggct cgtggccgaa tcggcgactg aacccctaat ctcggaaaaa ggagttcgtc
     1021 atgcgtttca ttcctactcg ggtgcacggc atcctggatt atctcgttgg tgtgttgctg
     1081 atctttgctc cgcggatctt cggtttcgaa aacaccggag ccgcttcgac ggtgccggtc
     1141 gttctcggca tcgcggcgct gatctatagc ctgctgacgc gttacgagct gggcttgatc
     1201 aaagtcctgc cgttcaaagc gcacttggcg atggattttg cgagcggtgt cgtgctgttg
     1261 gtttcgccat gggcgtttgg gttttcggaa accgtatggg tgccgcatgt gatcgttggc
     1321 ctgctggaaa tcggcgcggc cacgatgacg cgcacggtgc ccggcgatgt ggcgccgcac
     1381 gtgccggcga tgcgataaaa gatggcgacg atctgacggt tattcggtca actgaccaac
     1441 gatcagctga ccaaggatca ggtcacaggg cgcgtggggc acgcgccctg ttttgctttc
     1501 tggggttcca cctttgcagc tttctccttt gaggtcttca gctttggcga ctttgtctcg
     1561 ggcgtcgcgc cctcaggtgc tccgcgaagg aatttggctg gcggaggcgc gaagggtgat
     1621 gacgacggat ttcgaagcgg gagtgttgct ttcgtcggcg gtgctgcggt tgggcacgag
     1681 cgcgttcgcc tcgggaaaat acgcggcggt gcaacgacgg gggatgttgt aaggcgcgac
     1741 catgaactgc tcgacgtgtc gctgctcgcc ttcgaaatga ctcgtgatgt cgacgagctg
     1801 gccctgcacg aaaccggcgg cgcgcaggtc gtcttcgttc atgaagatca cgcggcggcc
     1861 gttgtagatg ccgcggtagc gatcatcgag tccgtagatc gatgtgttga actgatcgtg
     1921 actgcggatc gtggtgagca ggtaatggcc ttcgggaatc gcggcgcggg tgagcggcgc
     1981 cgctttgaaa atggctttac ccgagggcgt gttgaaacgg cgttcgtcac gcggtggatt
     2041 cggtaaatag aacgtgcctt cacgtatccg ttcgttgaat gcaggaaagc ctggcacaac
     2101 cgcttcgatg cgatcgcgga tgcgatcgta gttcgccacg tgggcgtccc agtcgagcgg
     2161 atacgtcggg ccgaggacgg ctttcgcaag accggcgacg atcgcggact cgctcaataa
     2221 tttttccgag ggcggctcga gattgccgcg cgaaggattg atgacgccca tgctgtcctc
     2281 gaccgtgacg aactgttcgc cgctcgcctg gcggtcgatg tcggtgcggc cgaggcacgg
     2341 aagaatcagc gcagcgcggc cggtgacgac gtgcgagcga ttgagcttcg tggcgacatg
     2401 cgcggtgagc cggcagttgc gcaggccgga cgcagtgagc tcggtgtcgg acgtggccga
     2461 aagaaaattt ccgcccatgg cgacgaacac ttttgcgcgg ccctcgttca tcgcgcggat
     2521 ggagtcgacg gtgtcgaggc cgtgctcgcg cggcggttcg aagcggaatt cggcggcgag
     2581 tttgtcgagg aactcttttt tcgggcgctc ccaaatcccc atcgtgcgat cgccttgaac
     2641 attcgagtgt ccgcgcacgg ggcagggacc ggcgccgggt ttgccgatgt ggccgccgag
     2701 caggaggaag ttcatgatct gctggatggt cgcgacggcg ttcttgtgct gcgtgatgcc
     2761 catgcaccag cagacgatga tgcgcttcga tgcggtcgcg atgtcggcgg cggcgcggat
     2821 ctgttcgcgc gagaggccgg agaaggaaac gatgtcgtcc cacgaagtcg cgcggagttg
     2881 cgcgatcaat tgctcgtaac cctccgtgtg atggcggatg aactcgtgat cgaagacctc
     2941 gccggggcgg cgttcttcct cggcgagcag ttctttcatc aagccgagga gaaacgccat
     3001 gtcgccgttg atcagcacgg gcagccagag atcggagagc gtcgtgccgc cggttaaaaa
     3061 gaagcgcggg acgcgcgtcg gatgcagaag gtcctgcgga tttttgaaac gctcggtgcc
     3121 ggtttctgga ggcggattga cgccgacgat tttgcaaccg cgcgctttcg cggcctgcag
     3181 cgacgtgagc atgcgcgggt gattcgtgcc gggattctgg ccgatgatga aaatggcgtc
     3241 ggccttctcg aagtcgctga gccgcacgca acctttgccg atgccgatca tctgtggcag
     3301 cgcgatgctc gtggactcgt ggcacatgtt ggagcagtcc ggcagattgt tcgtgccgaa
     3361 cgcgcgcacg aaaagttgcc agagaaacgc ggcttcgttg ctcgtgcggc cggaggtgta
     3421 gaaaacggct tcgtcggggg agccgagcgc tttcaactcg tcggcgatga ggcgaaacgc
     3481 gtcggaccag gcgatcggct cgtagtgcgt cgcaccgggg cgaagcacga gtggctcgat
     3541 caagcgacct tgatggccaa gccagtgatc ggatttcgcg gcgagttcgg cgacggaatg
     3601 ttggtgaaag aagtcggcgc cgatg
//
LOCUS       DASPLT010001335         1298 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_39991, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001335 DASPLT010000000
VERSION     DASPLT010001335.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1298)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1298)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1298
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_39991"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            53..>1298
                     /locus_tag="VEA63_13630"
     CDS             53..>1298
                     /locus_tag="VEA63_13630"
                     /inference="COORDINATES: protein motif:HMM:NF024240.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HYD85098.1"
                     /translation="MVVGGGLAGTCAAITAARAGLKVVLVQDRPVLGGNSSSEVRLWI
                     LGATSHMGNNNRWAREGGVIDEIIVENTFRNPEGNPLIVDTILLEKVIGEPNITLLLN
                     TAVFEATKHDAATIRSVRAFCSQNSTMYEIAAPLFCDASGDGVLGFMAGAAFRMGAES
                     QEEFGEKFAPTKEYGELLGHSLYFYTKDTGRPVSFTPPSYALQDITKIPRWRQFNARE
                     HGCKLWWIEYGGRLDTVHATEQIKWELWRVVYGVWNHIKNSGQFPEAANLTLEWVGTI
                     PGKRESRRFEGDYMLRQQDIVEQRTHPDAVAFGGWSIDLHPSDGVFSEKPGCNQWHAK
                     GVYQIPYRCLYSRNVDNLFLAGRIISATHVAFGSSRVMATCSHAAQAVGEAAFVCRRD
                     KVLPRTLLEPIRMAELQRRLQRN"
BASE COUNT          251 a          422 c          395 g          230 t
ORIGIN      
        1 ctctccttcc gaacgtcgtg atctcaatac ccaacacctc accacggatc tcgtggtcgt
       61 cggcggcggg ctggccggga cgtgtgcggc gatcacagcc gcgcgcgccg gtctcaaggt
      121 tgtgctcgtg caagatcgcc cggtgctggg cggcaacagt tccagtgagg tgcgcctctg
      181 gatcctcggg gcgacctcgc acatgggaaa caacaaccgc tgggcgcgcg agggcggcgt
      241 gatcgacgag atcatcgtcg aaaacacctt ccgcaacccc gagggtaatc cgctcatcgt
      301 cgacaccatt ctgctcgaga aggtgatcgg cgaaccgaac atcacgctgt tgctcaacac
      361 cgcggtcttc gaggccacga agcacgacgc cgccacgatc cgcagcgtgc gcgcgttctg
      421 ctcccagaac tccacgatgt atgaaatcgc cgcgccgctc ttctgtgacg cctcaggcga
      481 tggtgtgctg ggcttcatgg ccggcgcggc gttccgcatg ggcgcggagt cgcaggaaga
      541 attcggcgag aaattcgcgc caaccaaaga atacggcgaa ctgctcgggc actcactcta
      601 cttttataca aaggatacgg ggcggccggt gtcgttcacg ccgccgagct acgcgctgca
      661 ggacatcacg aaaattccgc gatggcgcca gttcaacgcc cgcgagcatg gctgcaaact
      721 ctggtggatc gaatacggcg gccggctcga caccgtgcac gccaccgagc agatcaagtg
      781 ggaactctgg cgcgtcgttt acggtgtgtg gaatcacatc aaaaactccg gccagtttcc
      841 cgaggccgcc aacctcacgc tcgaatgggt cggcacgatt cccggcaaac gtgaaagccg
      901 gcgcttcgaa ggtgactaca tgctgcgcca gcaggacatc gtcgagcagc ggacgcatcc
      961 cgatgccgtg gcgttcggcg gctggtcgat cgacttgcat ccgtccgacg gcgttttcag
     1021 cgagaagccc ggctgcaatc aatggcacgc gaaaggcgtt tatcagattc cgtatcgctg
     1081 cctctacagt cgcaacgtcg acaacctctt cctcgccggc cgcatcatca gcgcgaccca
     1141 tgtcgccttc ggttcgagcc gcgtgatggc gacctgctcg catgccgcgc aagcggtggg
     1201 cgaagccgct ttcgtttgcc gacgcgacaa ggttctgccg cgcacgctcc tggagccgat
     1261 tcgcatggcg gagctgcagc gccgcttgca acgcaacg
//
LOCUS       DASPLT010001336         1544 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_220316, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001336 DASPLT010000000
VERSION     DASPLT010001336.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1544)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1544)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1544
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_220316"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..597)
                     /locus_tag="VEA63_13635"
     CDS             complement(<1..597)
                     /locus_tag="VEA63_13635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HYD85099.1"
                     /translation="MAREYQFPFELRHLVYFREVARQLHFRKAAESLAVAQPALSRAV
                     AQLETALDIDLLNRTRRGVEITPAGHLLLERIEPLLRALSLIPAELQALSSGQVGHIR
                     VAFTGLAMSTVLPGILREFNQRFPGVRVELNESPSSAQLAALESGALACGFFHPDAPT
                     PNLHTRLLLRERNGVLLPAAHPLAARSKLQLRDLAATPF"
     gene            813..>1544
                     /locus_tag="VEA63_13640"
     CDS             813..>1544
                     /locus_tag="VEA63_13640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HYD85100.1"
                     /translation="MAKSLFQKVWEAHTVRKLANGQTQLLIGTHLIHEVTSPQAFGML
                     RDLGLKVAFPERTFATVDHIVPTDQFVEPYRDPLAQAMMDELRKNCAEFGITFFDRAT
                     GKQGIVHIVGPEQGITQPGTTIACGDSHTSTHGAFGAIAFGIGTSQVRDVLATQTMAL
                     GTLKVRRINVNGKLRPGVYAKDVILHIIRILGVNGGTGFAYEYGGEVFDRFTMEERMT
                     VCNMSIEGGARVGYVNPDDTTFEYLK"
BASE COUNT          309 a          390 c          585 g          260 t
ORIGIN      
        1 gaacggcgtc gcggcgagat cgcggagctg gagtttggag cgcgcggcga gcggatgcgc
       61 ggcggggagg agaacgccat tgcgttcgcg caggagaagg cgcgtgtgga ggttgggcgt
      121 gggggcatca ggatggaaaa aaccgcacgc gagcgcgccg gactcgaggg cggcgagctg
      181 ggcggaggac ggcgactcgt tgagctccac gcgcacgccg ggaaaacgtt ggttgaactc
      241 gcgcaggatg ccggggagaa cggtcgacat cgcgagcccg gtgaacgcga cgcggatgtg
      301 gccgacctgg ccggaggaaa gcgcttggag ctcggcgggg atcagggaga gcgcgcggag
      361 gagcggctcg atgcgctcga ggaggaggtg gccggcgggc gtgatttcaa cgccgcggcg
      421 ggtgcggttg agaagatcga tgtcgagtgc ggtctcgagt tgcgcgacgg cacgactgag
      481 agcgggttgc gcgacggcga gcgactcggc ggctttgcga aaatggagct ggcgcgcgac
      541 ctcgcgaaag tagacgaggt ggcggagctc gaaagggaat tggtattcgc gagccatgga
      601 gaaagctgcg ctttcgtttc gagtttggaa tttcggattc ggagttggaa aaggcggtgg
      661 atcggctgat gcccgtgcgg catcactggg agtgaaagaa gtatttcccg gcatggggcg
      721 caactggtat tctatcccac gacttggact ggcaccgtgg ccggaaggct cgacaacgcg
      781 gaacgcggaa ccaagaactc ggaacacctt ttatggccaa atctcttttt caaaaagtct
      841 gggaagcgca caccgttcgc aaactggcga acgggcagac gcagttgctg atcggcacgc
      901 atctgattca cgaagtcacg agcccgcagg cgttcgggat gttgcgcgat ctcggattga
      961 aggtggcgtt tccggagcgg acgttcgcga cggtcgacca catcgtgccg acggatcagt
     1021 tcgtcgagcc gtatcgcgat ccgctggcgc aggcgatgat ggacgagttg cgcaagaatt
     1081 gcgcggagtt cggcatcacg tttttcgacc gcgccacggg caagcaaggc atcgtgcaca
     1141 tcgtcgggcc ggagcagggc atcacgcagc cggggacgac gatcgcgtgc ggcgactcgc
     1201 acacgagcac gcacggtgcg tttggcgcga tcgcgtttgg gatcggcaca agtcaggtgc
     1261 gcgatgtgct ggcgacgcag acgatggcgc tggggacgtt gaaggttcgc cggatcaacg
     1321 tgaacggaaa gctgcggccg ggcgtgtatg cgaaggacgt gatcctgcac atcatccgca
     1381 tcctcggtgt gaatggcggc accggcttcg cttacgaata tggcggcgaa gtgttcgacc
     1441 ggttcacgat ggaagagcgc atgaccgtgt gtaacatgtc gatcgaaggc ggcgcgcgcg
     1501 tcgggtatgt gaatccggac gacacgactt tcgagtatct gaag
//
LOCUS       DASPLT010001337         1593 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_76486, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001337 DASPLT010000000
VERSION     DASPLT010001337.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1593)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1593)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1593
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_76486"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1509
                     /locus_tag="VEA63_13645"
     CDS             <1..1509
                     /locus_tag="VEA63_13645"
                     /inference="COORDINATES: protein motif:HMM:NF019259.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1592 domain-containing protein"
                     /protein_id="HYD85101.1"
                     /translation="WMDVEGPLTDEDADAAAGYRLLFGELPLEKRESGAGVVMLDVVA
                     PRIRATVGPGGGGGFGLNVPLAKVAVDVVSPQPREDAERLLRGFMHRAYRRPVDAAHV
                     ARFLKLFETQFEQGAGFARSMLTAYTAVLASPGFLFVEEQPGRLDDYALATRLALFLW
                     NSEPDERLRALAENGELGQPGVLRAETERLLNDPKAERFVTAFTDYWLDLRKVDDTSP
                     SPTLYNDYELDDPLKQAALDETRLFVAELLKEYLPARMVVDSDFTFLNERLAAHYGVP
                     GVAGVQMRKVALPADSVRGGLLTQASVLKVTANGTTTSPVLRGNWITARILGYETPPP
                     PPVPSVEPDIRGAVTIRQQLEKHRADVSCASCHARMDPPGFALESFDVMGGWRKNYRA
                     VKDGVEPVKGIGMNGQAYAFHEALPVDATGELPDGRSFSDVREFKRLLIEDEVTVARN
                     LVKQLTIYATGAPVRFVDRPAIEEILARAQASGYGVRTIVHEIVQSELFQKK"
BASE COUNT          284 a          373 c          638 g          298 t
ORIGIN      
        1 tggatggacg tcgagggacc gctcacggat gaggatgcgg atgccgcagc gggctatcgt
       61 ttgttgtttg gggaactgcc gctggaaaag cgcgagagcg gggcgggtgt ggtgatgctc
      121 gatgtcgtgg cgccgcggat acgcgcgacg gtggggccgg gcggcggcgg tggttttggg
      181 ttgaacgtgc cgctggcgaa agtggcggtg gacgtagtgt cgccgcagcc gcgcgaggac
      241 gcggagcggc tgttgcgcgg gttcatgcac cgcgcgtatc ggcgaccggt ggacgcggcg
      301 cacgtggcgc gttttctgaa attattcgaa acgcagtttg agcaaggtgc ggggtttgca
      361 cgctcgatgc tcacggctta cacggcagtg ctggcttcgc cgggatttct gtttgtcgag
      421 gagcagccgg gacggctcga cgattatgcg ctggcgacgc ggctggcgtt gttcctgtgg
      481 aattcggagc cggacgagcg actgcgggcg ctggcggaaa acggtgaact cggtcagccg
      541 ggcgtgttgc gcgcggagac cgagcggttg ttgaacgatc cgaaggcgga gcggttcgtg
      601 acagcgttca cggattactg gctggatttg cgaaaggtgg acgacacgtc gccgtcgccc
      661 accttgtata acgattacga gttggacgat ccgctgaagc aggcggcgtt ggatgagacg
      721 cggctgttcg tggcggagct gttgaaggaa tatctgccgg cgcggatggt ggtggactcg
      781 gacttcacgt ttttgaacga gcggctggcg gcgcattacg gcgtgcccgg cgtggcgggc
      841 gtccagatgc ggaaagtcgc gctgccggcg gacagcgtgc gcggcgggct gttgacgcag
      901 gcgagtgtgt tgaaggtgac ggcgaacggg acgacgacct cgccggtgtt gcgcgggaac
      961 tggatcacgg cgcgtatcct tggttatgag acgccgccgc cgccgccggt gccgtcggtg
     1021 gagccggaca tccgcggggc ggtgacgatc cggcagcagt tggagaaaca tcgcgcggac
     1081 gtgagctgtg cgtcgtgtca tgcgcgaatg gatccgccgg gatttgcgtt ggaaagcttc
     1141 gacgtgatgg gcggatggag gaaaaattat cgcgcggtga aggacggcgt ggaaccggtg
     1201 aaagggatcg ggatgaacgg gcaggcgtat gcgtttcacg aggcgctgcc ggtggacgcg
     1261 acgggcgagt tgccggatgg gcggagtttt tcggacgtgc gtgagttcaa gcggctgttg
     1321 attgaagacg aggtgacggt ggcgcggaat ctcgtgaagc agttgacgat ttatgcgacg
     1381 ggcgcgccgg tgcgattcgt cgaccggccg gcgatcgagg agattttggc gcgcgcccaa
     1441 gcgagtggct acggcgtgcg gacgatcgtg catgagatcg tgcagagtga gttgtttcag
     1501 aagaagtgaa ggggcgaacg gagttcgaga gaacgagaac gagaacgaga acgattacga
     1561 ttacgattac gattacgagc acgagcacga gca
//
LOCUS       DASPLT010001338         1347 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_76488, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001338 DASPLT010000000
VERSION     DASPLT010001338.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1347)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1347)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1347
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_76488"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            117..>1347
                     /locus_tag="VEA63_13650"
     CDS             117..>1347
                     /locus_tag="VEA63_13650"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HYD85102.1"
                     /translation="MLSRRTELAEAEHFYRATLKLAPNYSAAQFQLGQLLATTGRDDE
                     ASTFYEQRLASEPADVLATLGLVDIERRHGRSESAIRRLENLLKAKPAAAEARLLLAE
                     ILDSSGRTEEASKHRIAAQSSGVATPTQDPWLDQLYLQSFDPFRLQTLGVIRLNAESA
                     ETALPFLRRAAKLAPDDGAIQDSLAVALRKNGQDQEARKVLETAVIRTNAMIVHLRLA
                     DLLTAQGDFAQAEALLRQGMAAHGDRAEFHDALGRLQSQASRFGEAVKSFAEALRLNP
                     TMTETRLNYGRALLAAGRREEAKNAVAQALESRPAWPEALLFLGELALEASDMTEASS
                     WAARALSADEKSSAARDLIARVALRRAADALVTDDQAEAEGIYREALERVPEQGALHG
                     ALGTLLGRQQRLSEAHAA"
BASE COUNT          275 a          424 c          424 g          224 t
ORIGIN      
        1 ggcggctcgc gacactctac tttgccaacg atctcgcgac ggaagcggaa cgcgctctgc
       61 gaacgcttca cacgctggaa ccgacgaacg cgcgatggcc gtatcttctg gctaccttgc
      121 tgagccggcg caccgaactc gccgaagccg aacatttcta tcgtgcaacg ctgaaacttg
      181 caccgaacta ttcagcggcg cagttccaac tcggccagct ccttgccacc accggtcgcg
      241 acgacgaagc gtccacattt tacgagcaga gacttgcgag tgaacctgcc gacgtcctgg
      301 cgacgctcgg tttggtagat atcgaacgcc ggcatggccg ctcggaatcg gccattcgtc
      361 gcctggagaa tctgctcaag gccaagccgg cagcggccga agcccgcctg ctcctcgccg
      421 aaattctgga ttcgtcgggg cgaacagaag aagcgtccaa gcaccggatc gcggcgcaga
      481 gttcaggcgt ggcgacgccc acgcaggatc cgtggctgga tcaactctac cttcaatcct
      541 tcgatccgtt ccggcttcaa acgctgggtg tgatacgcct gaatgcggaa tctgccgaga
      601 ccgcgctgcc gttcctgcgc cgggctgcaa aattggcgcc ggacgatggc gcgatacagg
      661 attcgctcgc ggtggcgctg cgaaaaaacg ggcaagatca agaggcccgc aaggtgctcg
      721 aaaccgccgt aatccgaaca aacgcgatga tcgtccatct ccggctggcg gatctgctca
      781 cagcgcaagg cgacttcgcc caagcagaag cacttcttcg ccaaggcatg gcggcccatg
      841 gcgatcgcgc cgagtttcac gatgcgcttg ggcggttaca gtcccaggcc agccgctttg
      901 gggaagcggt gaaatcattt gcggaagcgc tgcggctgaa tccgacaatg acggaaacgc
      961 gactcaacta tggtcgggcg ctgctggcag cgggtcgtcg cgaagaggcg aagaacgcgg
     1021 tcgcgcaagc cttggaatcg agaccggcct ggccggaggc gctgctgttt ctcggggaac
     1081 tcgccctcga ggcctccgac atgacagaag ccagctcatg ggctgcgcgc gcgctgagcg
     1141 ccgacgagaa atcatccgcc gctcgcgatc ttatcgccag ggtagcgctc agacgggccg
     1201 cggacgcact cgtcacagat gatcaagcgg aggccgaagg gatttaccgc gaagcgctgg
     1261 aacgtgtgcc ggagcagggc gcattgcatg gcgcgctcgg caccttgctc gggcgacagc
     1321 aacgactgtc cgaagcgcat gccgcgt
//
LOCUS       DASPLT010001339         1192 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_200961, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001339 DASPLT010000000
VERSION     DASPLT010001339.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1192)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1192)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1192
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_200961"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..209)
                     /locus_tag="VEA63_13655"
     CDS             complement(<1..209)
                     /locus_tag="VEA63_13655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD85103.1"
                     /translation="MKKIKSRHVSLLAVASLIAPAALAQTQGAAETAANAANLVGNIA
                     GNAAQAAAAAANTAADAATTAAANAA"
     gene            649..>1192
                     /locus_tag="VEA63_13660"
     CDS             649..>1192
                     /locus_tag="VEA63_13660"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HYD85104.1"
                     /translation="MLVVGVFSSIAFASLRVSAGEALRFDPERLGRLDRVIEEHVARR
                     DLAGAVMFIARDGKPVKLTAYGLQDIEADKPMAEDAIFRIASMSKAVTTVAALMLYEE
                     GKFLLREPLSKYLPAFENSMVAVPAPEGSPAGTKFVLEKAKRPIWIRDLMTHTAGLTY
                     GGGGDLAAELYREANVQGWNF"
BASE COUNT          191 a          295 c          468 g          238 t
ORIGIN      
        1 gcggcgttgg cggcagcggt cgtggcggcg tcggcggcgg tgttggctgc cgcggcggcg
       61 gcttgggcgg cgttgccggc aatgttaccg acgaggttcg cggcgttcgc ggcggtttcg
      121 gcggcgcctt gagtttgcgc gagcgccgca ggggcaatca acgaggcgac ggcgagaagg
      181 gagacgtggc gggatttgat ttttttcaca ggaatgccgt caggggcgtt cgcgatagac
      241 ctgatgaacg cgatgggttc tagcgttgaa ttggtgcggc ggctcacgcg cgaggtcggg
      301 gcgttgcgaa cgatctgcac acgattgcgg aagtgcgctg cgtgtttttt gcgcctgtta
      361 gccgagccgg cgaaatcgca ggaggatacg caacgcggcg ggaatcggaa cgcagcagtt
      421 acgcgagtga ggtcgatgaa gtcgcgcatc ccggatgaag gtctcccggg gtaatttggg
      481 aaatcaccgg atgcctggga agcggtgcgc tcccagcgtt cggtccggag acgcgcggat
      541 tggcaggccg cgagctggtt ccggggtggc aaaatttttc gtgcggacga tcgctgaagc
      601 ggcaatctcg cgcgcatgca tcacccctgc tcccggtttc cgcgttggat gttggtcgtc
      661 ggcgttttca gctcgatcgc tttcgcgtca cttcgtgtga gcgcgggcga ggcgctgcgt
      721 ttcgatccgg agcggcttgg acggctggat cgcgtgatcg aggagcatgt ggcgcggcgc
      781 gatctggcgg gcgcggtgat gttcatcgcg cgcgacggca agccggtgaa gctgacggcg
      841 tatggcttgc aggatatcga ggcggacaag ccgatggcgg aggacgcgat ttttcggatc
      901 gcgtcgatga gcaaggcggt gacgacggtc gcggcgctga tgttgtatga agaggggaaa
      961 tttttgctgc gcgagccgtt gtcgaaatat ctgccggcgt tcgagaattc catggtggcg
     1021 gtgccggcgc cggagggctc gcccgcaggg acgaagttcg tgctcgagaa ggcgaagcgg
     1081 ccgatctgga ttcgggattt gatgacgcac accgccgggc tgacgtatgg cggcggtggc
     1141 gatctggcgg cggagcttta tcgggaagcg aatgtgcagg gctggaattt ca
//
LOCUS       DASPLT010001340         1660 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_16586, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001340 DASPLT010000000
VERSION     DASPLT010001340.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1660)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1660)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1660
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_16586"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(90..>1660)
                     /locus_tag="VEA63_13665"
     CDS             complement(90..>1660)
                     /locus_tag="VEA63_13665"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HYD85105.1"
                     /translation="LIVENAREYAIFSTDLERRVTSWNVGAERLLGYTETEIVGQSAD
                     IIFTPEDRAKNAPEREAGTALRDGRAANERWHLRKNGTRFWGSGVVMAMQNRNGSILG
                     LLKIMTDQTEARATAEALERSRSDLLAALQTAEQARAEAVAAGRAKDQFLAALSHELR
                     TPLNPVMMIASEAAADPSLPAHVRADFEVVRKNVELEARLIDDMLDITRITQGKLALQ
                     AQQVDLHASVREAVAMLRTAASEKHIVVSFSLQAWHSQVLGDPARLQQIFANLLSNAI
                     KFTPSGGTVVVESANDETLNEIVVRVTDTGIGLTRDELTRVFQPFTQGGHAGESGSAL
                     YGGLGLGLTIVEELSVRHGGRVSAASPGPGQGATFSVSLPIARSVTAPPPMKDTVPEQ
                     TNAVTGLRILLVEDHAPTRMALQRMLARRNHTVISANSCAAALEEVKQRSFDLVISDI
                     GLPDGDGYELLGRLRETQPQLPGIALSGFGMEGDLRRSETAGFAVHLVKPVPMSKLDA
                     AISQLGIGAPSVRG"
BASE COUNT          293 a          532 c          530 g          305 t
ORIGIN      
        1 tgcggacgca cggtccgaga gaaaaatgga ggcttgcgga cgcgcacccg atgacgcgcg
       61 cagcgcgcgc ctcaccgccc gcctataatt tagccgcgaa cgcttggggc gccgattccc
      121 aactggctga tggctgcgtc gagcttggac atcggcacgg gtttcacgag gtgaacggcg
      181 aacccggcgg tctcggaacg tcgcaagtcg ccctccatgc cgaatccgct gagcgcgatg
      241 ccgggaagct gcggctgagt ttcgcgcaag cgaccgagca gttcgtagcc atcgccatcg
      301 ggcaatccga tatcggagat gacgaggtcg aaactccgct gcttcacttc ctccagcgcg
      361 gcggcacacg agtttgcgga gatcaccgtg tgatttcggc gggcaagcat gcgctgcaac
      421 gccatgcggg tgggcgcatg atcttccacc aggagtatcc gaagtccggt gacagcattc
      481 gtttgctcgg gcaccgtatc tttcatgggc ggcggtgcgg tgacactccg cgcaatcggc
      541 aggctcaccg aaaacgtcgc gccctggccg gggcccgggc tcgcggcgga gactcgtccg
      601 ccgtgacgga cactcagctc ctcgacgatc gtcaaaccca agccgagacc gccatacaac
      661 gctgagccgc tttcgcccgc atgcccgcct tgggtaaaag gctgaaacac acgcgtgagt
      721 tcgtcgcggg tcaggccaat gccggtatcc gtgacccgca cgacaatctc gttcaaggtc
      781 tcgtcattcg cgctttcgac cacgaccgtt ccgcctgaag gagtgaactt gatggcgttg
      841 gagagcaggt tggcaaaaat ctgctgcaga cgcgccgggt caccgagcac ctgcgaatgc
      901 caggcctgaa gggagaacga cacgacgatg tgcttctcgg atgcggccgt gcgcaacatc
      961 gccacggctt ccctgacgct cgcgtgaagg tcgacctgct gcgcctgcaa cgccagcttg
     1021 ccttgcgtga tgcgggtgat gtccagcatg tcgtcgatca gcctcgcttc gagttcgaca
     1081 tttttccgca cgacctcgaa gtcggcacgg acatgagccg gcagcgacgg atctgcggcg
     1141 gcctcgctgg cgatcatcat caccggattc aagggcgtgc gcaattcgtg cgacaacgcc
     1201 gcgagaaact gatctttggc gcggccggcg gcgacggctt ccgctcgcgc ctgttcggcg
     1261 gtttgcaggg ccgcgagcaa gtcgctacgc gagcgttcca gcgcttcggc ggtggcgcgc
     1321 gcctccgtct gatccgtcat gatcttcagc aatccgagga tggagccatt gcgattctgc
     1381 atggccatga cgacgccgct gccccaaaaa cgcgtgccgt ttttccgcag atgccagcgc
     1441 tcgtttgcgg cgcgtccgtc gcgcaacgcg gtgcccgcct cgcgttcggg ggcattcttc
     1501 gcgcgatcct cgggcgtgaa aatgatgtcg gccgactggc cgacgatttc cgtctcggtg
     1561 tagccgagca accgttcggc accgacgttc cagctcgtga cgcgccgctc gagatcggtg
     1621 gagaagatgg cgtattcgcg cgcgttctcg acgatcaggc
//
LOCUS       DASPLT010001341         1089 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_200969, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001341 DASPLT010000000
VERSION     DASPLT010001341.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1089)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1089)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1089
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_200969"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..553)
                     /locus_tag="VEA63_13670"
     CDS             complement(<1..553)
                     /locus_tag="VEA63_13670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD85106.1"
                     /translation="MAPTEHPHAVASHGTLFQFSRRALYFFGALLLLPWVVVGWLVID
                     VRAPRRSTTAASVPPTFGYDEYILKSKPGPWGELQSSRLLIEPPEEFMAAEVDASGPS
                     RWLFPGYTPDQLTALWQKASLSPAQIALLDASLREAPVPNAILLEPPASLILELSPES
                     RATIYTALSAFLENPAQNEPFRFR"
     gene            688..>1089
                     /locus_tag="VEA63_13675"
     CDS             688..>1089
                     /locus_tag="VEA63_13675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007360012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HYD85107.1"
                     /translation="MLDAKLIRETPDLVRAAIAKKHLEVDLDRVIAIDAEWRSVIREV
                     ETLRAQQKAANTAMAALPKGSPEFTAKVAEMKSVSQQVKERDGQLKEVEERFRQAMLS
                     LPNLPHASVPEGRTPEQNVVYATNGDADAPRP"
BASE COUNT          223 a          306 c          396 g          164 t
ORIGIN      
        1 cgcggaaacg aaacggctcg ttttgcgcgg ggttctcgag gaatgccgac aacgcggtgt
       61 agatcgtcgc gcgcgactcc gggctgagct cgagaatcag cgacgccgga ggttcaagca
      121 ggatcgcgtt tggaaccggt gcctcgcgca gcgacgcatc gagcagtgcg atctgggcgg
      181 gcgaaaggga ggccttttgc cagagcgcgg ttagctggtc gggtgtgtag ccggggaaga
      241 gccagcgtga agggccggaa gcgtcgactt ccgcggccat gaattcctcg ggaggttcga
      301 tgaggaggcg tgacgattgc aattcacccc acggccccgg cttggatttc aggatgtatt
      361 catcgtagcc gaaggtcggg gggacggagg ccgccgtggt cgagcgccgc ggagcgcgga
      421 catcgatcac cagccagccg acaacgaccc aggggagcag cagcagggcg ccgaaaaaat
      481 aaagggcgcg gcgggaaaac tggaagagtg tgccgtgcga cgcgacggca tgagggtgtt
      541 cggtgggggc caaggcggtg agcgtgcgat tagggcaagc agggcgcggg agtccacggg
      601 atttcgcgga ttacgagcac ggcacgctgc ggccgcgcag gatttttgtt tgttctaccc
      661 cgagcccccg ccatacctga ccccttcatg ctagacgcca aactcatccg tgaaacgccc
      721 gaccttgtgc gggcagccat cgccaagaag cacctcgagg tcgatctcga ccgagtgatc
      781 gcgatcgatg ccgaatggcg ctcggtgatt cgcgaggtcg agacgctgcg cgcacagcag
      841 aaggcggcca acaccgcgat ggcggcactg cccaaaggtt cgcctgagtt caccgcgaaa
      901 gtggcggaga tgaagagcgt ttcgcagcag gtgaaggagc gggacgggca actgaaggaa
      961 gtcgaggagc gcttccggca ggcgatgctt tccttgccca acctgccgca tgcgtcggtc
     1021 ccggaaggcc gcacgccgga gcagaacgtc gtgtatgcaa caaatggaga cgcggacgcg
     1081 ccgcggccg
//
LOCUS       DASPLT010001342         1136 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_117637, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001342 DASPLT010000000
VERSION     DASPLT010001342.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1136)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1136)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1136
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_117637"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            193..591
                     /locus_tag="VEA63_13680"
     CDS             193..591
                     /locus_tag="VEA63_13680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD85108.1"
                     /translation="MASFLMVLGISTSIIAMQMGFRNLDVARGNTLASQIMQSEIERL
                     RLMSFTQITALPASDTVDLSTMFTTNTALAARFTVTRTVATDGTRADVRYITVSVQWN
                     SYDGKSHTRSFTSMYAKDGLYDYYYTLAKT"
     gene            560..1120
                     /locus_tag="VEA63_13685"
     CDS             560..1120
                     /locus_tag="VEA63_13685"
                     /inference="COORDINATES: protein motif:HMM:NF019575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HYD85109.1"
                     /translation="MITTIHSPRRDRACAGFTLVEMMISATLGGLILVAVLSSFLFLG
                     RSGWAIQSYNDMERQARSALEVFAEDTRQASAVTWHSATSVTLVVNSTQVTYTYSGDA
                     LTRTSSGNTRMLVDGITAFSFLAYTINGTQISDFSSAAARIIANNTTKQIQISLAAAR
                     SRQTVKDATNTVLSARFILRNKRVTA"
BASE COUNT          218 a          394 c          305 g          219 t
ORIGIN      
        1 gacatttgcc gtgcggctta cctgctaaaa agcggccccc tgcctctccc gcggctgacg
       61 tcatgcgcgt agactacgtg catgggttca cccattcgat ttcgttctcg gtccgccggt
      121 ggagttgatt cttcgcggga gggccatcgc ctgccgcgct gcgcccgcgg cttcacgatc
      181 gccgaggtgg cgatggcgag cttcctcatg gtgctcggga tttccacctc gatcatcgcg
      241 atgcagatgg gattccgtaa cctcgacgtc gcccgcggca acaccctcgc ctcgcaaatc
      301 atgcagagcg aaatcgagcg gttgcgactc atgagcttca ctcaaatcac cgcgctcccg
      361 gccagtgaca ccgtcgacct cagcacgatg ttcaccacca acaccgcgct cgccgcacga
      421 tttaccgtca cgcgcacggt cgcgaccgac ggcacccggg cggacgtgcg ctacatcacc
      481 gtttccgtgc agtggaacag ctacgacggg aaatcccaca cgcgctcgtt cacgagcatg
      541 tatgccaagg atggtcttta tgattactac tatacactcg ccaagacgtg accgtgcgtg
      601 cgcggggttc acgctcgtcg agatgatgat ttccgccacg ttgggcggac tcatcctcgt
      661 ggcggtgctg tcgtcattcc tcttccttgg ccgcagtggc tgggcgatcc aaagctacaa
      721 cgacatggag cgccaggcgc gaagtgcgct ggaggttttc gccgaagaca cccgtcaggc
      781 aagcgcggtg acttggcata gcgcgacgtc cgtcaccctc gtggtcaact ccacgcaggt
      841 cacctacacc tattccggag acgcgctgac gcgcacctcg agcggcaaca cccgcatgtt
      901 ggtcgacggc atcaccgcct tctcattcct cgcctacacg atcaacggca cgcagatttc
      961 ggatttcagc tccgcggcgg cccgcatcat cgccaacaac acgaccaagc aaatccagat
     1021 ctcgctggcc gctgcccgtt cgcggcaaac cgtgaaagac gccaccaaca ccgtcctctc
     1081 cgcccgcttc atcctgcgca ataaacgcgt caccgcttga acgctctgcc atgaaa
//
LOCUS       DASPLT010001343         1630 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_261577, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001343 DASPLT010000000
VERSION     DASPLT010001343.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1630)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1630)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1630
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_261577"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            51..1319
                     /locus_tag="VEA63_13690"
     CDS             51..1319
                     /locus_tag="VEA63_13690"
                     /inference="COORDINATES: protein motif:HMM:TIGR02601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter-associated beta strand
                     repeat-containing protein"
                     /protein_id="HYD85110.1"
                     /translation="MQRCLRHACGLVFSWLLGPLPAHAISWIGSNGDAFNATSSWGGA
                     VPSGANSTAEFGGNTGGVIIQLNHAVGTFYFNSSYTSGGFIVRNGGSLSLINGIVNDS
                     AVSPEFTIRDGTVFFTGNAPKLGNATFNVESAGLLKLFTANHPNGSAARVNLTGGQIG
                     MNNTTDGGSASLGEMKGTSGRISLPDVALTVGALNTDATYSGTIDSLGALTKVGTGTW
                     TLTGANTYDGSTLISAGTIKIDNASGSAFGTGNVTIGIGGTLTGAGSFTGALENNGVY
                     APGNSPELALHSSFSQGATGILEMEIAGLTRGIGYDALNVSGALTFGGSLQLSLIDGF
                     DAASLTIGDTFNLFDWGSASGTFSFLDLPDLSAHGLTWDISALYTTGELGVSAIPEPS
                     TYAMLAGAAAMVFAAWRGRRGRSGRAEIMR"
     gene            1323..1553
                     /locus_tag="VEA63_13695"
     CDS             1323..1553
                     /locus_tag="VEA63_13695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD85111.1"
                     /translation="MKPHVLRHVVDHVADPVEDEFVWTMRNVGIEHVLLGSDYRSIHY
                     NRTWTHWSACRSSKGRRRKSSTRDARALFGLK"
BASE COUNT          307 a          542 c          489 g          292 t
ORIGIN      
        1 cgtcacggcc tgctggccga agccctctcg gcgccatgta agatcgggcc atgcaacgct
       61 gcctccgcca tgcctgcggc ctcgtgtttt catggctgtt aggtccactg ccggcccacg
      121 ccatctcgtg gatcggctcg aatggagacg cgttcaacgc cacctcctcg tggggcggcg
      181 cggtgcccag cggcgccaac agcaccgccg agtttggcgg gaacacaggt ggggtgatca
      241 tccaactcaa ccacgcggtc ggcacgtttt acttcaattc gagctatacc agcggcggct
      301 tcatcgtgcg gaacggcgga agcctctcgc tcattaacgg catcgtgaac gactcggcgg
      361 tgagcccgga attcacgatc cgcgacggca ccgtgttctt cacgggcaac gccccgaagc
      421 tcggcaacgc cacctttaac gtcgaaagcg ccggcttgct gaaactcttc accgcaaacc
      481 atccaaacgg cagcgccgcc cgcgttaacc tcaccggcgg tcagatcggc atgaacaaca
      541 cgaccgatgg cggctccgcg tcgctcggag aaatgaaggg cacgagcggc aggatcagcc
      601 tccctgacgt cgcgctcact gtcggcgctt tgaacaccga cgccacttac tccgggacga
      661 tcgacagctt gggagcgttg acgaaggtcg gcaccggcac ctggacgctc accggcgcga
      721 acacctacga cggatcgacg ctgatttccg cgggcacgat caagatcgac aacgcttccg
      781 gctccgcgtt cggcaccggc aacgtcacca tcggtatcgg cggcacgctc acgggcgcgg
      841 gctcgttcac cggcgcgctc gagaacaatg gcgtctatgc acccggcaac agccctgagc
      901 tcgcgttaca ctcgtccttt tcgcaaggcg cgacgggcat tctcgaaatg gaaatcgccg
      961 gcctgacccg cggcatcggt tatgatgcgc tcaacgtttc cggcgcgctc actttcggcg
     1021 gctcgctcca actcagtctc atcgacggct tcgatgccgc gtcgctcacg atcggcgaca
     1081 cctttaattt gttcgactgg gggagcgcgt cgggaacgtt cagcttcctc gacctgcccg
     1141 acctctccgc gcacggcctc acctgggata tttccgcgct ctatacgacg ggcgaacttg
     1201 gcgttagcgc gattcccgag ccctcgacgt atgcgatgct cgctggcgcc gcggcgatgg
     1261 ttttcgccgc gtggcgtggc cgtcgcgggc gaagcggccg agctgagatc atgcgctaaa
     1321 aggtgaagcc ccacgtcctc cgccacgtcg tcgatcatgt agcggacccg gtcgaggacg
     1381 aattcgtctg gacgatgcgc aatgtcggaa tcgagcacgt gctgctgggt tcggactacc
     1441 gcagtattca ctacaacaga acgtggacgc actggagcgc ctgccgctcg agcaaaggga
     1501 gaaggcgaaa atcctctacg agagacgccc gcgctttgtt tggcctgaag taacgccaga
     1561 accgtcgaac gggtcaggcc ggaacatatt caaaagctgt ggccgactcc cgtagcggca
     1621 ggcctccgtg
//
LOCUS       DASPLT010001344         1274 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_117656, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001344 DASPLT010000000
VERSION     DASPLT010001344.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1274)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1274)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1274
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_117656"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(36..1133)
                     /locus_tag="VEA63_13700"
     CDS             complement(36..1133)
                     /locus_tag="VEA63_13700"
                     /inference="COORDINATES: protein motif:HMM:NF016241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF481 domain-containing protein"
                     /protein_id="HYD85112.1"
                     /translation="MKKSLIALVGAAALIVSAVSLHADVLRLKNGDILSGTITQREGG
                     VVTIKGNLYGEVSVQESDVASFEVGTGVTAATTSATAAVPVTAAATAAAAGGVASATI
                     PAAAAPAADPNKATWSRSLTVGGSYVSAPYKQGVVPGTAPGVTGAQLGQLGDQYSVQI
                     SGAIIRATAKNAISLEGSYTYADYEPYPSPVADTYSATLAWNHALSPRHYTVSRTSYM
                     ADEVKNIDHSFYQIVGVGWKLIDTTATKLDFVPGLMLSHEKKNFVFDNQWEPGYGFLQ
                     NLTHAFNPAMSFEQRLTFRNLFEDAYYYTVDAYVGFKGMLSATIGVQVGVTYNYDNGA
                     DRRAFIAGPGLVFYPSNQGVLSLSTGFQFKF"
BASE COUNT          263 a          448 c          341 g          222 t
ORIGIN      
        1 agcgttcgaa gttcagcctt cgaactccgc ccgccctaga acttgaactg aaaccccgtc
       61 gagagcgaca acacgccctg gttgctcgga tagaatacga ggcccggccc tgcaataaac
      121 gcccggcgat ccgcgccgtt atcgtaattg taggtcaccc ccacctgcac gccgatggtc
      181 gcactgagca tgcccttgaa acccacatac gcatcgaccg tgtaataata cgcgtcttcg
      241 aaaagattcc gaaaggtcag tcgctgctcg aacgacatgg caggattgaa cgcatgcgtc
      301 aggttctgca ggaagccgta acccggctcc cactggttat cgaaaacgaa attcttcttc
      361 tcgtgcgaga gcatcaggcc cggcacgaag tccagcttcg tcgccgtcgt gtcgatcagc
      421 ttccagccca cgcccacaat ctgataaaag gagtggtcga tgttcttcac ttcgtccgcc
      481 atgtaactcg tgcggctcac cgtgtaatgc cgcggtgaca gcgcgtgatt ccacgccagc
      541 gtcgccgaat acgtgtcggc caccgggctc ggatacggct cgtaatccgc atacgtgtaa
      601 ctcccctcca gcgaaatcgc attcttcgcc gtcgcccgga tgatcgcgcc cgagatctgc
      661 accgaatatt ggtcgcccaa ctgcccgagc tgcgcgcccg tcacacccgg cgccgtgccc
      721 ggcacgactc cctgcttgta gggcgccgac acatacgaac cgccgaccgt caacgaacgc
      781 gaccacgtcg ccttgtttgg atccgccgcg ggagccgccg ccgccggaat cgtggccgac
      841 gccacgccac cagccgccgc cgccgtagcc gccgccgtga ccggaaccgc cgcggtcgcg
      901 ctcgtcgtcg cggctgtgac gcccgtgccg acttcgaacg acgccacatc gctctcctgc
      961 accgaaacct cgccgtagag attgcccttg atcgtcacca ccccgccctc ccgctgggtg
     1021 atcgtgcccg ataaaatatc gccattttta aggcgaagaa catccgcgtg aagtgaaacg
     1081 gcggatacga taagggcggc ggcgccaacg agcgcgatca gggatttttt catagctgga
     1141 acggccgcga cagtgcgacg ccgcccgccc ctcgcgcaaa gcaaaagatt atcgctgaca
     1201 gatattgggc gaggaccgcg caaagccccc ccgcccttca accgccttcc gcgaaacgca
     1261 aaatcgcgtc cgcc
//
LOCUS       DASPLT010001345         2092 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_117669, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001345 DASPLT010000000
VERSION     DASPLT010001345.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 2092)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2092)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2092
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_117669"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            561..1013
                     /locus_tag="VEA63_13705"
     CDS             561..1013
                     /locus_tag="VEA63_13705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD85113.1"
                     /translation="MSVAPITAVNQTLFNDEVTYDGVADIAFIAIQSANGKFGGIRTG
                     NTTYYASQGFTGVYAPGVDVAGPVNIGDVSAFDSATPVLVFGSAGTVTIAGGNLEQAN
                     AQPVKVSGIARVQFVDGTTSQGVSLPAQINAGVFEADGVDITDLLVTL"
     gene            1582..>2092
                     /locus_tag="VEA63_13710"
     CDS             1582..>2092
                     /locus_tag="VEA63_13710"
                     /inference="COORDINATES: protein motif:HMM:NF012787.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HYD85114.1"
                     /translation="MNPLLFAGALCAFFTATLFAADDSLLDKDGNPAGFHTLAIGDPA
                     PDFTLPGIDGRNWSLNDFSEHDILMVLFTSNHCPTSHGIEQRLKKLRADMRGKSFGLV
                     AINPNHPDGLRLDELGFGEFNDSFEEMKPYAAQQGWDFPYLYDGDKQLTARAYGCLAT
                     PHVFIFDKER"
BASE COUNT          374 a          682 c          611 g          425 t
ORIGIN      
        1 gttattcgat tagccgtcac agctcgcaca aactccgcgt gctttcgccg ggattcgatg
       61 agcgacgttt tccaagcgtg cgcaggcggc ggtgagccga atcgcctgca agcgggcacc
      121 taccttcgaa ctcgcgcagc ctccattaca gtggctgttc aggtaggtgg cgcgctcgcc
      181 gcgtcagacg ttgcgcgcga gcgcgcaacc tacggaagca ggccggcaga gacgccggcc
      241 gccacggttg aaagtggctc tgcgcgtgag cgcagggagt ttgcgcgtcg tcccgccacc
      301 gtccgcaaca aagccgatgg atcaaaaccc cactcccttt ttgcgccttc tgcgtctttt
      361 tgcggccagc acatccgagg atcggaaacc tccattcttg tatcttcggc tttccgccac
      421 cgcgcgccgt tcgcatcggc gcctatcccc gcaacagtcc gttgggtgac ttaatccgtc
      481 cgactcgcgc cgtcgtattt caggatgctc gcacgcgggc tggtgatcac ggggccaacg
      541 agacgtcgaa catctcggta atgagcgtag cgccaattac ggcggtgaac cagacgctgt
      601 tcaacgatga ggtgacctac gatggcgtcg ccgacatcgc gttcatcgca atccaaagcg
      661 cgaatggaaa attcggcggt atccgcacgg gaaatacgac ctactatgcg agccagggct
      721 tcaccggcgt gtatgcgccg ggcgtggacg tggccggacc ggtgaacatc ggcgacgtaa
      781 gtgccttcga ctccgcgacg ccggtgctgg tgttcggcag tgccggcacg gtcacgatcg
      841 ccggcggcaa cctggagcag gccaacgccc agccagtgaa agtaagcggg attgcgcgcg
      901 tgcagtttgt ggacggcacc acatcgcagg gagtgtcgtt gcccgcgcag atcaatgccg
      961 gcgtgttcga agccgacggc gtcgacatca cggacctgct cgtcacgctt taggctagca
     1021 acctgccggc gggtttcagc aatgcaccgc cccgcgttct gcgccgattc tgcgttgtgc
     1081 cggcaacccg gctcacctgc caacgctgct gacgctttgc gcggcgcagt cgatctacag
     1141 cggttcaaaa aattccatcg ccaaattttg gccgcaaaga ggtgcaaaag gcgcaagaac
     1201 agaagagtcc tccgagtcat ttttgagcct tctgtgcctt tttgcggcta tagttttgat
     1261 ctgctccagc ctccgggttg cacccgcacg gtcagattga ccgtgttact ttgcaggtcg
     1321 tcagacgttt ccggctgatg cggaatccct cgagaagcgg gctcaccaga gggctggcgc
     1381 gggatttcgg ttcgagttcg ctcggccatc gtcttcaacc ttcgtgccga cgttgctcgc
     1441 gaatcctttc cgcctgctac gcgtctcgcg gggagcgttg ccggccctgc ggcggcgcgt
     1501 tgcgctcggc ccggttgtgc gcttcctgaa tgaccgtttc gtccccaagc cccctcgatg
     1561 caaatgcttc cccgtttcac catgaacccc ctcctcttcg ctggcgcgct gtgcgccttc
     1621 ttcaccgcca ctttgttcgc cgccgacgac tcgctgctcg acaaggacgg caatcccgca
     1681 ggctttcaca ccctcgcgat cggcgatccg gcgcctgatt tcacgctgcc gggcatcgac
     1741 ggccgcaact ggtcgctgaa cgatttctcg gagcacgaca ttctcatggt cttgttcaca
     1801 tcgaaccatt gccccacctc gcacggcatc gagcagcggt tgaaaaaact tcgcgcggat
     1861 atgcggggca aaagcttcgg cctcgtcgcg atcaatccca atcaccccga cggactgcgc
     1921 ttggacgagc tcggcttcgg cgagttcaac gactccttcg aagagatgaa gccttacgcg
     1981 gcgcagcaag gctgggactt tccgtatctc tacgacggcg acaagcagct caccgcgcgc
     2041 gcctacggct gcctcgcgac gccgcacgtg ttcattttcg acaaggaacg cc
//
LOCUS       DASPLT010001346         2984 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_16691, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001346 DASPLT010000000
VERSION     DASPLT010001346.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 2984)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2984)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2984
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_16691"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(85..1227)
                     /locus_tag="VEA63_13715"
     CDS             complement(85..1227)
                     /locus_tag="VEA63_13715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007360456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYD85115.1"
                     /translation="MSHRPIRLFRPGVIAALLLLSACTKNSDTIKIGEYASLSGKEAS
                     LGQSSHRGTLLAIETINAAGGVLGKQLELITEDTQSKAGESGTAVRKLISRDKVVAIL
                     GEVASSRSLEGAPIAQAAKVPMVSPASTNPQVTEIGDYIFRVCFIDPFQGPVMAKFAL
                     NTLKAKRIGLMTSASSAYSVGLAKFFREAYLAGGGTIAAEQKYQEGDKDFKAQLTAIR
                     AAGVDAIFNPGYYNEGALMVKQARDLGITVPMFGADSWEAQALIELGGKAVEGVYLCS
                     HYSPDDPSPRVQEFVTAYRKRWGDDAAPDSNASLGYDSALVLADAIRRAGSTDRAKIR
                     DALAATKDFSAVTGTITIDKNRDASKSAVIITVRDGRFQYVETIDP"
     gene            1469..2581
                     /locus_tag="VEA63_13720"
     CDS             1469..2581
                     /locus_tag="VEA63_13720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007359961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HYD85116.1"
                     /translation="MNTYDRHLLREWMEILAMVVCATLGLLLVQVLYDDFRDLRELGA
                     RGLDLWMYVAVTIPSYLALVLPLALLVSLMFVLGKLHRANELTALRAAGVSLLRITAP
                     VWLAGVLACGLSWWLNTRVVPWSIEESAKVKDNLIFRKESGVLAPDRVAAVYSVGFDN
                     PNGARMWFFNRYSQFARRGYGVSVSILGDQRRELSRLVAEEAWLDEARGGWVFKNGRE
                     LTFNPENSELVASAAFGERVETEFDEDPTLMLLIDRRPKDLSLPQLRRLIEYFSAQRN
                     PKGTPYAVRYFSLIADTLAPLIVIAIAIPFAVSGVRVNPAVGVSKSIGLFFLYYIFAN
                     VASSLAARGIIDPLTAAWVPNVGMLGVAGWFLLRLR"
     gene            2789..>2984
                     /locus_tag="VEA63_13725"
     CDS             2789..>2984
                     /locus_tag="VEA63_13725"
                     /inference="COORDINATES: protein motif:HMM:NF024879.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper domain-containing protein"
                     /protein_id="HYD85117.1"
                     /translation="MKTLLVTLALAAATVGTARAEYYRPSIVRDSTIVGAVAGALIGG
                     HNGDRWAQGALIGAAAGAVVG"
BASE COUNT          490 a          759 c         1117 g          618 t
ORIGIN      
        1 aggatgacgg cggtgaatag caggacgagg gggcgacagg attgcatggg tgtgggaaaa
       61 cgaggacgcg gcgcatgcgt ccggtcaggg atcgatggtt tcgacgtatt ggaagcggcc
      121 gtctctcacc gtgatgatca cggcgctctt cgacgcgtcg cgatttttgt cgatcgtgat
      181 cgtgccggtg acggcggaga aatctttcgt ggccgcaagc gcgtcgcgga tttttgcgcg
      241 atcggtggag ccggcgcggc gaatggcgtc ggccagcacg agcgcggaat cgtagccgag
      301 cgaagcattg gagtcaggtg cggcatcgtc gccccagcgt tttctgtaag ccgtgacgaa
      361 ctcctgcacg cgcggcgaag gatcatcggg ggaatagtgc gagcaaaggt aaacgccttc
      421 gaccgctttg cctccgagct cgatgagcgc ctgcgcttcc cagctatcgg cgccgaacat
      481 cgggacggtg atgccgagat cgcgtgcttg tttcaccatg agggcgccct cgttgtaata
      541 gccgggattg aagatcgcgt cgacgcccgc ggcgcgaatc gcggtgagct gcgctttgaa
      601 atccttgtcg ccctcctggt atttttgttc ggcggcgatg gtgccgccgc cggcgaggta
      661 ggcttcgcgg aaaaatttgg cgaggcccac gctgtaggcc gacgaggcgc tggtcatgag
      721 cccgatgcgt ttcgccttga gggtgttgag ggcgaacttg gccatgaccg ggccttggaa
      781 cggatcgatg aagcagacgc ggaaaatgta atcgccgatc tccgtgacct gagggttggt
      841 cgacgcgggc gaaaccatcg ggaccttcgc cgcttgggcg atcggcgcgc cttcgagcga
      901 gcggctcgag gcgacctcgc cgaggatggc gacgactttg tcgcgagaga tgagtttgcg
      961 gacggcggtg ccgctttcac cggccttcga ctgcgtgtcc tcggtgatga gctcgagctg
     1021 tttgccgagg acgccgccgg cggcgttgat ggtttcgatg gcgaggagcg tgccgcggtg
     1081 cgaggactgg ccgagactgg cttctttgcc ggagagggag gcgtattcgc cgatcttgat
     1141 ggtgtcggag ttcttcgtgc aggccgacag gagcaggaga gcggcgatga cgccgggccg
     1201 aaagaggcgg atgggacggt gagacatgaa gaaaagagcg ccaagaggac gcgaccgggc
     1261 gcagacaagg gaaggcaaag caggttttca accaaactgt tccggtcgtt gcgcgggaga
     1321 attgtgagat cgagctggaa aacggtgagc ggggcgaatg cgctcgcgat tgagtttcgc
     1381 gagcttgtcg agctggggaa acgcgaaggc gcgggagtgg ttggattcgg ttttgcgttc
     1441 ggccgggcgc gcggtttgat ccgccgggtt gaacacctac gaccgacatt tgctgcgcga
     1501 gtggatggag attttggcga tggtggtctg cgcgacgctc gggctgctgc tcgtgcaggt
     1561 gttgtatgac gattttcgcg acctgcggga gttgggcgcg cgcgggctgg atttgtggat
     1621 gtatgtggcg gtgacgatcc cgagctatct ggcgctggtg ctgccgctgg cgctgttggt
     1681 gtcgttgatg ttcgtgctcg gaaaattgca tcgggcgaac gagctcacgg cgttgcgggc
     1741 ggcgggggtg agtctgttgc ggatcacggc gccggtgtgg ctggcgggcg tgctggcgtg
     1801 cgggttgtcg tggtggctga acacgcgcgt ggtgccgtgg tcgatcgagg aatcggcgaa
     1861 ggtgaaggac aacctgatct ttcgcaaaga atcgggcgtg ctcgcgccgg atcgcgtggc
     1921 ggcggtttac agcgtgggtt tcgataatcc aaacggggcg cgcatgtggt ttttcaatcg
     1981 ctacagtcag ttcgcgcggc gcggttacgg cgtgagcgtg tcgatcctcg gtgaccagcg
     2041 gcgcgagtta tcccgcttgg tggcggagga ggcatggttg gacgaggcgc gcggcggatg
     2101 ggtgttcaag aacgggcgcg agctgacgtt caatcccgag aacagcgagt tggtggcgag
     2161 cgcggcgttt ggggaacgcg tcgagacgga gttcgacgag gatccgacgc tgatgttgct
     2221 catcgatcga cgaccgaaag acctgtcgct gccgcaattg cgacggctga ttgaatattt
     2281 ctcggcgcag cggaatccga aggggacgcc ttacgcggtg cgctatttca gtttgatcgc
     2341 agatacgctg gcgccgctga tcgtgatcgc gatcgcgatt ccgttcgcgg tgagcggcgt
     2401 gcgggtgaac ccggccgtgg gcgtgtcgaa atcgatcggg ttgttctttc tctattatat
     2461 ttttgcaaac gtggcgtcgt cgctggcggc gcgggggatc atcgatccgc tcacggcggc
     2521 gtgggtgccg aacgtcggca tgttgggcgt ggcggggtgg tttttgctgc ggctgcggta
     2581 gtggggcgtc gcgcagatat ttttcgtaat tcgcgactct tccgtccgcg aggacgcctc
     2641 ggagaggcgt ccctaccggt gctaccgcgc cgcaggattt agtaacattt ctcgagggac
     2701 accacactcg gtgaacgcaa ctggctggcg cgcgggggta gcaaagaatt ttggtaaacc
     2761 tttttgaggc cgtggcgtct cattgcgcat gaaaaccctc cttgttaccc tcgcactcgc
     2821 ggcggcgacc gtcggcacgg cccgcgcgga gtattatcgt ccgtcgatcg tgcgcgactc
     2881 gaccatcgtg ggcgcggtcg ccggtgcgtt gattggcggg cacaatggcg atcgttgggc
     2941 gcaaggtgcc ttgatcggcg cggcggccgg cgcagtcgtc ggcg
//
LOCUS       DASPLT010001347         1253 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_97199, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001347 DASPLT010000000
VERSION     DASPLT010001347.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1253)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1253)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1253
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_97199"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..485
                     /locus_tag="VEA63_13730"
     CDS             <1..485
                     /locus_tag="VEA63_13730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007359323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DALR anticodon-binding domain-containing
                     protein"
                     /protein_id="HYD85118.1"
                     /translation="YADLSQNRSSNYVFSWEKMLALEGNTAPYLLYAVARIHSIFRKA
                     GFTLPSSSDAHCHIMYDISAVAASATPPETATEIALARKLVKFPDAIRLATDTLRPHF
                     LGLYLFELAGDYSSFNNADKVLVDDPVIRSRRLLLCARTLLILETGLHLLGLRTLTRI
                     "
     gene            687..>1253
                     /locus_tag="VEA63_13735"
     CDS             687..>1253
                     /locus_tag="VEA63_13735"
                     /inference="COORDINATES: protein motif:HMM:NF025597.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4339 domain-containing protein"
                     /protein_id="HYD85119.1"
                     /translation="MATQEFYIRNESDTEARGPFTLEQLSSLTESGQLTPATLYYEAN
                     TEQWTAIEANAELKATLFPEKKKLSIRTKQNLETLNKESEGAAPITVGDMLAAAEGRT
                     ADTKDKQDPAEAMARAAAIGRFAAIAALVVASAGEMLPSAKAIMAMDTTALMESPLVF
                     LGVLDLFLAVVLGLGVVSLYPFIRFRAAL"
BASE COUNT          249 a          449 c          318 g          237 t
ORIGIN      
        1 aatacgccga tctttcccag aatcgcagca gcaactacgt gttctcctgg gaaaagatgc
       61 tcgcgctcga gggcaacacc gcgccctacc tcctctacgc cgtcgcgcgc atccattcca
      121 ttttccgcaa agccggcttc acgctcccct cgagctccga tgcccattgt catataatgt
      181 atgacatttc cgcggtggcc gcctccgcga ccccgccgga aaccgccacg gaaatcgcgc
      241 tcgcccgcaa gctcgtgaaa tttcccgacg ccatccgtct cgcgaccgac acgctccgcc
      301 cccatttcct cggcctctat cttttcgagc tcgctggcga ctacagctcg ttcaacaacg
      361 ccgacaaggt cctcgtcgac gacccagtca tccgctcccg ccgactgcta ctctgcgccc
      421 gcacgctgct gatcctcgaa accggcctcc acttgctcgg tttgcgcacg ctcacccgaa
      481 tttagggagg aaagctccaa gcaccaacat ccaagctcca gagaagcttc aatgagctaa
      541 atttcaaaag ctgatcggat tggaattagg tggtttatgt ttcaatggag cttggatgtt
      601 ggagcttgga gcttccggct gccgccgccg ggcccggccc actaaactct tgccgctccg
      661 cgcggccgtc gacagcttca ccgcgcatgg cgacgcagga attctacatc cgcaacgagt
      721 cggacacgga ggcccgcggg cccttcaccc tcgaacagct cagctcgttg accgagtccg
      781 gccagctcac gccggcgacg ctttattacg aggcgaacac cgagcaatgg accgccatcg
      841 aagccaacgc cgagctgaaa gcgacgctct tccccgagaa gaagaaactc agcatccgca
      901 ccaagcagaa cctcgaaacg ctcaacaagg aaagcgaagg cgccgcgccc atcacggtcg
      961 gcgacatgct cgccgctgcc gaaggccgca ccgccgacac gaaggacaaa caagatcccg
     1021 cggaagcgat ggcacgcgcc gccgccattg gtcgcttcgc cgccatcgcc gccctcgtcg
     1081 ttgcttccgc cggagaaatg cttccgtccg cgaaagcgat catggcgatg gacaccaccg
     1141 ctttgatgga gagcccgctc gtcttcctcg gcgtgctcga cctttttctc gccgttgttc
     1201 tcggcttggg cgttgtctcc ctgtatccat ttattcgatt ccgggccgcg ctc
//
LOCUS       DASPLT010001348         2143 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_180612, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001348 DASPLT010000000
VERSION     DASPLT010001348.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 2143)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2143)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2143
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_180612"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..454
                     /locus_tag="VEA63_13740"
     CDS             <1..454
                     /locus_tag="VEA63_13740"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HYD85120.1"
                     /translation="ELKGREPGDEIEGLARRLYERGMAMSGQDAMWGWQQLRLMNRQI
                     LQRFESWDVYLTPVMNTPAPRVDYLDTLTDDLREFDKRQAATFGFTPPFNMTGQPSLS
                     LPLAQSQSGLPIGMMFTAKYADEATLFRLAGQLEKEIPWKDRKPAVWG"
     gene            605..1111
                     /locus_tag="VEA63_13745"
     CDS             605..1111
                     /locus_tag="VEA63_13745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD85121.1"
                     /translation="MFTIAPVGVGREQLYAFESGGFLPANPDPSRVTHLADGSTMAPI
                     ESLFEEEFTRLRDQLSRIPNLVCVVVDPLPRRGDPCLHGYAETIFYAGDDVYHVIRSA
                     TSEAAAERALSASPPWSSLNVLSTIAPTLDDNRHTELSELRKCAGAVVEMSCGAYDGE
                     GYVNWRRG"
     gene            1119..2141
                     /locus_tag="VEA63_13750"
     CDS             1119..2141
                     /locus_tag="VEA63_13750"
                     /inference="COORDINATES: protein motif:HMM:NF022427.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2804 domain-containing protein"
                     /protein_id="HYD85122.1"
                     /translation="MQTKLETGDLLDARGRLIARGYATREVRKYARGAIKAWPLRIKE
                     WDYYCVLTDRYGLALTIADNDYLGFLGATWLDFEKRTYVSEEALLSFPLGGMHLPQSA
                     DAGDIVQQHAKLSIAYRHVVDGGREISVSAPTFAGGQGLKGKLFLEQPPMDRMVIATP
                     FANAPRSFYYNQKINCLRASGAVTIGDQTFAFEPGSAFGVLDWGRGAWTYDNTWYWAS
                     ASGIAEGRSFGFNVGYGFGDTSAATENMLFIDGVAHKLTDVAFHLPEGAADSAPWRFT
                     SSDNRFELTFEPIVDRAATTDIGLIKSDQHQVFGRFSGRVMLDDGSAIRVENLIGFAE
                     RVHNRW"
BASE COUNT          404 a          714 c          629 g          396 t
ORIGIN      
        1 tgaactcaag ggccgcgagc ccggcgacga aatcgaaggc ctcgcccgcc gcctctacga
       61 acgcggcatg gcgatgagcg ggcaagacgc gatgtggggc tggcagcaat tgcgcctgat
      121 gaatcgccaa atcctgcagc gtttcgaaag ctgggatgtc tatctcaccc cggtgatgaa
      181 tacgcccgcg ccgcgcgtcg attatctcga tacgctgacc gacgatctcc gcgagttcga
      241 caaacgccaa gccgccacct tcggcttcac cccgcccttc aacatgacag gccagccttc
      301 gctctcgctg ccgctggcgc aaagccaaag cggcttgccg ataggcatga tgttcaccgc
      361 aaagtacgct gacgaagcga ctctcttccg cctcgccggc cagctcgaaa aggaaatccc
      421 gtggaaggat cgcaagcccg ccgtgtgggg ttgaccgatg acgcgtgttc tcgaacccgt
      481 tgatctggac gatagaggcg cggcttacct ccggacgcgt ctatctgaac ttgccgcgca
      541 tggcccatcg ttgtccgcgc tctgcgatgt gctggctgag cgtctgcgga ccgataccgg
      601 caccgtgttc acaatcgctc cggtcggcgt cggtcgtgaa cagctttacg cgttcgagtc
      661 tggtggattt ttgccagcga accctgaccc ctcgcgcgtc actcatctgg ctgacggcag
      721 cacgatggcg ccgatcgaaa gcttgtttga ggaggagttc acgcggctgc gcgatcaact
      781 ttcacgcata cccaacctcg tttgcgtcgt cgttgaccct ctacctcgta gaggagatcc
      841 gtgtttgcat ggctacgccg aaacgatctt ctacgccggg gatgacgtgt atcacgtgat
      901 caggtcagcg acctcggaag cggcggctga gcgcgcgttg agcgccagcc cgccgtggtc
      961 cagtctcaac gtgctctcga cgatcgcccc gacgctcgac gacaatcggc acaccgaact
     1021 ctcggagctt cgcaagtgcg ctggcgccgt cgtcgagatg tcgtgcggcg cttatgacgg
     1081 tgaaggctac gtcaactggc ggcggggata ggacctcaat gcaaacaaaa ttggaaacgg
     1141 gcgacctgct cgacgcccgc ggccgcctca tcgcccgcgg ctacgcgacc cgcgaagtgc
     1201 gcaaatacgc gcgcggcgcc atcaaggcgt ggccgctgcg gatcaaggag tgggactatt
     1261 attgcgtcct caccgatcgt tacggcctcg cgctcacaat cgccgacaac gattatctcg
     1321 gcttcctcgg cgccacttgg ctcgatttcg agaagcgcac gtacgtcagc gaagaagcgc
     1381 tactgtcctt cccgctcggc ggcatgcacc tgccccagag cgccgacgcc ggcgacattg
     1441 tgcagcagca cgccaagctc tcgatcgcct atcgccacgt cgtcgacggc ggccgcgaga
     1501 tcagcgtcag cgcgccgact ttcgcgggcg ggcagggcct caaaggcaag ctcttcctcg
     1561 aacagccgcc aatggaccgc atggtgatcg caacgccgtt cgcgaacgcg ccgcgctcat
     1621 tctactacaa ccagaagatc aattgcctac gcgcaagcgg cgccgtcacc attggcgacc
     1681 agaccttcgc cttcgagccc ggcagcgctt tcggcgtgct cgattggggc cgcggcgcct
     1741 ggacttacga caacacctgg tattgggctt ccgcctcggg catcgccgaa gggcgctcgt
     1801 tcggcttcaa tgtcggctat ggcttcggtg acaccagcgc cgccaccgag aacatgctct
     1861 tcatcgacgg cgtcgcccac aagcttaccg atgtcgcatt tcacctgccc gaaggcgcgg
     1921 cggattcagc gccctggcgc ttcaccagca gcgacaatcg gtttgagctc accttcgaac
     1981 cgattgtaga ccgcgccgct accaccgaca tcggcctcat caaatccgat cagcaccaag
     2041 tcttcggccg cttcagcggc cgcgtcatgc tggacgatgg gtcagccatc cgcgtcgaaa
     2101 acctgatcgg cttcgccgaa agggtgcata atcgctggtg agg
//
LOCUS       DASPLT010001349         1172 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_139767, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001349 DASPLT010000000
VERSION     DASPLT010001349.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1172)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1172)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1172
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_139767"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..439
                     /locus_tag="VEA63_13755"
     CDS             <1..439
                     /locus_tag="VEA63_13755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HYD85123.1"
                     /translation="RFAASLFVLAASTGGLVLNNVLKAIFGRERPDETLHLVEIDSLS
                     FPSGHAMLSATIYLTLAVLLTRLADRRREKSYLLGAALLLSFAVGLSRVYLGVHYPTD
                     VIAGWAAGIAWAQICWFVAHMIDRRRLAGAATEDTAPAASERL"
     gene            complement(954..>1172)
                     /locus_tag="VEA63_13760"
     CDS             complement(954..>1172)
                     /locus_tag="VEA63_13760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD85124.1"
                     /translation="GVMPSPDTVNIRDEDYPSEGKPLPAESPAAKALHEHPPQGHDQL
                     EVGSAGDAARPSKTGIMKPTLPPRSSRL"
BASE COUNT          232 a          327 c          362 g          251 t
ORIGIN      
        1 acggtttgcc gcgtcgctgt tcgtgctcgc tgcttccacc ggcggactgg tcttgaacaa
       61 cgtcctgaaa gcgatcttcg gtcgcgagcg cccggacgaa acgttgcatc tggtcgagat
      121 cgattcgttg agctttccca gcggtcacgc gatgttgtcg gcgacgattt acctgacgct
      181 cgcggtgctg ctcacgcggc tggccgatcg ccgtcgtgag aaaagttacc ttctcggcgc
      241 ggcgctgctg ctgagcttcg cggtcggatt gagccgtgtt tatctcggcg tccactatcc
      301 gacggacgtg atcgcgggct gggccgcggg catcgcgtgg gcgcagattt gctggtttgt
      361 ggcgcacatg atcgatcggc gccgtcttgc cggcgccgca accgaagaca ccgcaccggc
      421 tgcatccgag cgactttgaa gcgcgtggcg gtgcgctgcg actcatccga cgcccggatg
      481 cgctcgcgcg aattccggat gcgctgcgtc gattttcaga ggagaaatcg cgtttccgcg
      541 cgtatccagt tttcaaatat cggcgcacgt tgtgtgtgtc cttggcgtga aaaatgggaa
      601 gcggaggcga aaaaaaatcg tccgtgacgc attttcgatt ggaaaaaaat ttgaggtgtg
      661 cgtgcggtct acagtttaca aaacgcggtg atgcacgcca atttgggccc gcgcgatgaa
      721 gcttcccgct tcatcgttaa gcgaaaaccc tagaacaaag gaaaaaccaa atggctaaga
      781 aagcatcgaa atccgcaaag aagagcgctc cgaagaagaa ggcagccgcg aagaagaaga
      841 agtaaggctg acacttcacc gcgcgtttct tccgttccgc ttcatcgcgg aggtccgagg
      901 aaagcgcggg gtttcttgga aataggcgcg cgattttcgc gcgcctattt catttataaa
      961 cgcgatgagc gcggcggcag agtgggcttc atgatccccg tcttcgaggg acgcgccgca
     1021 tcgcccgcag aaccgacttc gagttgatcg tgcccttgcg gcggatgttc gtgcaacgct
     1081 ttcgccgccg gcgattccgc cggcagaggt ttgccttcac tgggataatc ctcgtcgcga
     1141 atgttcaccg tatccggcga aggcatgacg cc
//
LOCUS       DASPLT010001350         2559 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_201158, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001350 DASPLT010000000
VERSION     DASPLT010001350.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 2559)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2559)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2559
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_201158"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..489
                     /locus_tag="VEA63_13765"
     CDS             <1..489
                     /locus_tag="VEA63_13765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HYD85125.1"
                     /translation="IGTEEGKGNALITETNDLLRRMSDLLNYAGPIEGFHVFAEHVDV
                     VVCDGFVGNIMLKSWESLVKFFSGMLREELQANPVRATGALLSKGAFAALKERINPER
                     YGGAPLLGLKGNILKAHGSSNRHAIKSAIFAASRIIRADLNQRIEADIARANDLLTVP
                     VA"
     gene            531..1523
                     /locus_tag="VEA63_13770"
     CDS             531..1523
                     /locus_tag="VEA63_13770"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009513238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HYD85126.1"
                     /translation="MAHASTVILGTGAYAPARVLSNDELSLTVDTSDEWIRTRTGIRE
                     RRIAAAGEQTSDMGVKAAQAALADAKLTPHDIDLLIVATITPDLLMPAAACIIQQKLG
                     LRTDVACFDLNAACSGFVYALDTACAMLTSGRYRRALVIGVEKLSTIVDWQDRTTCVL
                     FGDGAGAAVIGLSDQPNIGLLGTKLGAFGESVELLWVPNGGSANPSTSDSIAANGHCI
                     KMKGKEVFKLAVRAMDEAARDILEQHHLTADQIALVIPHQANLRIIEAISDYLELPME
                     RFFVNVDRYGNTSAASIPIALDEARRAGRIKPGDVTLLVAFGAGLTYASALLRW"
     gene            1536..>2559
                     /gene="bamD"
                     /locus_tag="VEA63_13775"
     CDS             1536..>2559
                     /gene="bamD"
                     /locus_tag="VEA63_13775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0065003 - protein-containing complex
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HYD85127.1"
                     /translation="MRLFPAVRALPFWLLISGLGLVFAASARADIVWHPDTGWQIEGG
                     VLAGVTGPQARNALELMNRAREAEEDGSRGSAIRAYRKVSKRYPSSVYASEALYRTGK
                     LYLARKQYFKSFTAFATVLAGYPNTKYFNDIVGEEYRIASSLLDGARNRYWGIIPGFK
                     NRTRALDYFEVIVQSAPYSDYAPLALMNKARGQLRERDIIEAIDSLDRMINLYPQSLL
                     APDAYLKLAQTFTELVDGPRYDQGSTKEAITYFEDFLILFPNDENVPVAAKGLDEMKQ
                     VLSESKIEIGDFYYHKRRNYTAARVFYNEAITAYPDSPVAERAKQKLAQVEASASGQP
                     VPGAEKR"
BASE COUNT          485 a          963 c          661 g          450 t
ORIGIN      
        1 atcggcaccg aagagggcaa gggcaacgcc ctcatcaccg aaaccaacga cctgctccgc
       61 cgcatgtccg acctgctcaa ctacgccggc ccgatcgaag gtttccacgt gttcgccgaa
      121 cacgtggacg tggtcgtgtg cgacggtttc gtgggcaaca tcatgctcaa gagctgggag
      181 tcgctcgtga aatttttctc cggcatgctc cgcgaggaat tgcaggccaa ccccgtccgc
      241 gccaccggcg cactcctttc caaaggcgcc ttcgcggcgc tcaaggagcg catcaatccc
      301 gagcgatacg gcggcgctcc cctcctcggc ctgaagggca acatcctgaa agcccacggc
      361 tcttccaatc gccacgcgat caaaagcgcc atcttcgccg ccagtcgcat catccgcgcc
      421 gatctcaacc agcgcatcga ggccgacatc gctcgtgcca acgacctcct caccgtgccc
      481 gtggcctgac gcgccgtctt cgcgcccgcc acctgccagc gaccattctc atggcccacg
      541 cctccactgt tattctcggc accggcgctt acgctcccgc ccgcgtgctg agcaacgacg
      601 aactttccct tacggtcgac acctccgacg aatggattcg cacgcgcacc ggcattcgtg
      661 aacgccgcat cgccgcagcc ggcgaacaaa cgtccgacat gggcgtgaaa gccgcccaag
      721 ccgcgctcgc cgacgcgaag ctcacgccgc acgacatcga tctcctcatc gttgccacca
      781 tcacgccgga cctcctgatg cccgccgccg cgtgcatcat tcagcaaaag ctcggcttgc
      841 gcaccgacgt cgcgtgcttc gatctcaacg ccgcctgctc cggctttgtc tacgcgctcg
      901 acaccgcctg cgcgatgctc acctccggcc gctaccgccg ggcgctcgtg atcggcgtcg
      961 aaaaactctc cacgatcgtc gactggcagg atcgcaccac gtgcgtgctg ttcggcgacg
     1021 gcgccggcgc agcggtcatc ggcctcagcg accagcccaa catcggcctc ctcggcacca
     1081 agctcggcgc tttcggcgaa agcgtcgaac tcctctgggt tcccaacggc ggcagcgcca
     1141 atccctcgac cagcgattcc atcgccgcga acggccattg catcaaaatg aagggcaagg
     1201 aggtcttcaa actcgccgtc cgcgcgatgg atgaagccgc ccgggatata ctggaacaac
     1261 accatctgac cgcggatcag attgccctcg tgatcccgca tcaggcgaac ctgcgtatca
     1321 tcgaggcgat ttccgactat ctcgagctcc cgatggagcg ctttttcgtc aacgtcgatc
     1381 gctacggcaa cacctccgcc gcctcgattc ccattgcgct cgacgaagcg cgccgcgccg
     1441 gccggatcaa gccgggcgat gtcacgttgc tcgtggcatt tggggcgggc ttgacctacg
     1501 caagcgcgct gcttcgctgg taaatctttt ctctgatgcg tctcttcccc gctgttcgcg
     1561 cccttccttt ctggcttctg atttccggcc tcggcctcgt tttcgccgcc tccgctcgcg
     1621 ctgacatcgt gtggcatccg gacaccggct ggcagatcga gggcggcgtc ctcgccggcg
     1681 tcaccggccc tcaagcccgc aacgccctcg agctgatgaa tcgcgcccgc gaagccgagg
     1741 aagacggcag ccgtggctcc gccatccgcg cttatcgcaa agtttccaag cgttatccgt
     1801 cgtccgtcta cgcctcggag gcgctctacc gcaccggcaa gctctacctc gcgcgcaagc
     1861 aatacttcaa atcgttcacc gcgttcgcga ccgttctcgc cggctatccc aacacgaaat
     1921 attttaacga catcgtcggc gaggaatacc gtatcgccag ctcgctcctc gacggcgccc
     1981 gcaaccgcta ctggggcatc atccccggct tcaagaaccg cacgcgcgcc ctcgactact
     2041 tcgaggtgat tgtccaaagc gcgccctaca gcgactacgc ccccctcgcc ttgatgaaca
     2101 aggcccgcgg ccagctccgc gaacgcgaca tcatcgaggc aatcgattcg ctcgatcgga
     2161 tgatcaatct ttacccgcaa agcctcctcg cgcccgacgc ttacctgaag ctcgcgcaaa
     2221 ccttcaccga actggtcgac ggcccccgct acgaccaggg ctcgaccaag gaagcgatca
     2281 cctacttcga ggatttcctc attctcttcc ccaacgacga aaacgtcccc gtcgccgcca
     2341 agggcctcga cgagatgaaa caggtcctct ccgagagcaa aatcgagatc ggcgacttct
     2401 actaccacaa gcgccgcaat tacaccgccg cgcgcgtctt ctacaacgaa gccatcaccg
     2461 cctatcccga ttcgcccgtc gccgaacgcg ccaaacaaaa gctcgcccaa gtcgaagcca
     2521 gcgcctccgg ccagcctgtc cccggcgccg aaaagcgca
//
LOCUS       DASPLT010001351         1288 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_97251, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001351 DASPLT010000000
VERSION     DASPLT010001351.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1288)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1288)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1288
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_97251"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1288
                     /locus_tag="VEA63_13780"
     CDS             <1..>1288
                     /locus_tag="VEA63_13780"
                     /inference="COORDINATES: protein motif:HMM:NF012431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HYD85128.1"
                     /translation="ILVQQLEHWGVEVIFGLPGDGINGIMESLRNARERIRFVQVRHE
                     ESAAFMACAYAKYTGKLGVCLATSGPGGLHLLNGLYDAKLDGQPVLAITGHHFHDLID
                     THAQQDVDLDRVYMDVAVYNARVMGPEHIAAVTNLACRTALARRGVAHINFPVDTQSM
                     PVKNAPRSERNIKGHSSSDVFARRAGLPVKQDVEHAVEILNAGKRIAILAGQGALHAE
                     VELTAIAERLGAPIIKALLGKACVSDDSPYTTGGIGLLGTRPSQEALEECDTLLMVGT
                     SFPYMEFLPKPGQARIVQIDLDPARIGLRCPVEVGLAGDARTCLHVLLPLLDYHDDRR
                     FLERAQKGMREWWKLIEERGTRRDKPMKPQVVAWELGRQIANNAIVSCDSGTVATWFA
                     RQMPVRRGQMYSLSGTLASMANGLPYTIAAQIAYPNR"
BASE COUNT          237 a          424 c          389 g          238 t
ORIGIN      
        1 acatccttgt tcagcaactc gagcattggg gcgtcgaggt cattttcggc ctgcccggcg
       61 atggcatcaa cggcattatg gagtcattgc ggaacgcccg ggaacgcatc cgttttgtcc
      121 aggttcgcca cgaggaatcc gcggcgttca tggcctgcgc gtatgccaaa tacaccggta
      181 aactcggcgt gtgtctcgcc acctccgggc cgggcggctt gcacctgctc aacggccttt
      241 acgatgcgaa gctcgacggc cagccggtgc tcgcgatcac cggccaccat tttcacgatc
      301 tgatcgacac gcatgcgcaa caggacgtcg atctcgatcg cgtttacatg gacgtggccg
      361 tttacaacgc gcgcgtaatg ggacccgagc acattgccgc ggtcaccaat ctcgcttgcc
      421 gcaccgccct cgcgcgtcgc ggcgtggcgc atatcaactt cccggtcgat acccaatcga
      481 tgccggtgaa aaacgcgccg cgctccgaac ggaacatcaa gggacattca tcttccgatg
      541 tgttcgcgcg ccgtgccggt ctgccggtga agcaggacgt cgaacatgcc gttgagattt
      601 tgaacgcggg aaaaagaatc gcgatcctcg ccggccaggg cgcgttgcac gccgaagtcg
      661 aattaacggc gatcgcggag cgtctcggcg cgcccatcat caaagccctg ctgggcaagg
      721 cgtgcgtgtc cgacgactcc ccctacacca ccggcggcat cggcttgctc ggcacgcggc
      781 cttcgcaaga agccctcgag gaatgcgaca cgctgctgat ggtcggcacg tcgtttccct
      841 acatggagtt tctgccgaaa cccggccagg cgcgcatcgt gcaaatcgac ctcgatcccg
      901 cccgcatcgg attgcgttgt ccggtcgagg tcggtctcgc cggcgatgcg cgcacttgcc
      961 tgcatgttct ccttccgctc ctcgactacc atgacgatcg tcgctttctc gagcgggcgc
     1021 agaaaggcat gcgcgaatgg tggaagctga ttgaggagcg cggcacgcgg cgcgacaaac
     1081 cgatgaagcc gcaggtcgtc gcgtgggaac tcggccgcca aatcgccaac aatgcgatcg
     1141 tgagctgcga cagcggcacg gtggcgacgt ggttcgcgcg ccagatgccc gtgcgtcgcg
     1201 gtcagatgta ttcgctctcc ggcacgctcg cgtcgatggc gaacggcctc ccctacacca
     1261 tcgccgcgca gatcgcgtat ccaaaccg
//
LOCUS       DASPLT010001352         1762 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_97258, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001352 DASPLT010000000
VERSION     DASPLT010001352.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1762)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1762)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1762
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_97258"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1762
                     /locus_tag="VEA63_13785"
     CDS             <1..>1762
                     /locus_tag="VEA63_13785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HYD85129.1"
                     /translation="INLQSTRTAGSVLGRITQQVIDDGTYVRGTPLINLDLNGNGSIG
                     YLEYQQASPVRGNLNAGNTPLLQNFALPRDANGVPVPLNEVAGIRGIPQAMYDYLVAN
                     PQYDPTGQLRAQGVGGPLPLSGYIPIGMVLDPLTVGFDKLDLHRAAAFEREQDAKIAT
                     AFFDLSYDTDPDFQIKNQLFVDYIDSYKLSDMGLYGPRDTYAFEEKITVTKRIPSERL
                     PTWLAVNALASANYRLTNTLIDGGSFYNDYTANRVDAMAPTWIASRGGATPNTTFVSS
                     GVNSDLNNDGAPATSGNESEFYETGLGVLLDVDLFEKLNLVLGGRIDGSGAKNTDFAG
                     VFNPTTGTSANPGSFRATELSAKGWDTGTSWSTSATYRLPFNSRVYVTFANASLTLDS
                     AANSISNTVIAAGHIGSAELKEAGFKTSVLNDKLFFTAAAYEQTRANVASPTDPTATV
                     EVSATRSRGWEVELKWVPAKTFFLSVYARKQKTIYSPNTGAVIPVHARALGFMDVVDP
                     VTGAIIYPAEAFLYGGRAQIVLPADVKEYEEQQGRPNTQLGFNANYEFGGRYGITLSG
                     NYLSRTYSGRLKLIELPESYI"
BASE COUNT          356 a          664 c          447 g          295 t
ORIGIN      
        1 catcaatctc caaagcacgc gcaccgccgg ttccgtgctc ggccgcatca cgcagcaggt
       61 gatcgacgac ggcacctacg tgcgcggcac gccgctcatc aatctcgatc tcaacggcaa
      121 cggttccatc ggctatctcg agtatcagca agcctcgccc gtgcgcggca atctcaacgc
      181 cggcaacacg ccgctccttc agaatttcgc gctgccccgc gatgccaacg gcgttcccgt
      241 tccgctcaac gaagtcgccg gcatcagggg catcccgcaa gcgatgtatg actacctcgt
      301 cgccaatccc caatacgacc cgaccggcca gttgcgcgcc cagggcgtcg gcggtccgct
      361 gccgctctcc ggttacattc cgatcggcat ggtgctcgat ccgctgaccg tcggcttcga
      421 taaactggac ctccatcgcg ccgccgcctt cgaacgcgag caggacgcga agatcgccac
      481 cgcgttcttc gatctgagct acgacaccga cccggatttc caaatcaaga accagctctt
      541 cgtcgactac atcgacagct acaaactttc cgacatgggc ctctacggtc cgcgcgacac
      601 ctacgcgttc gaggaaaaga tcaccgtcac gaaacgcatt ccttccgagc gcctgcctac
      661 gtggctcgcc gtcaacgcgc tcgcttccgc caactaccgc ctcacgaaca cgctgatcga
      721 tggcggctct ttctataacg actacaccgc aaatcgcgtc gatgcgatgg cgcccacgtg
      781 gatcgcgagc cgcggcggcg cgacgcccaa caccactttc gtttcctccg gcgtaaactc
      841 cgatctcaac aacgacggcg ctcccgcgac cagcggcaac gaaagcgaat tctacgagac
      901 gggcctcggc gttctcctcg acgtcgatct gttcgagaaa ctcaacctcg tgctcggcgg
      961 ccgcatcgac ggctccggcg cgaagaacac ggatttcgcc ggcgtcttca atcccaccac
     1021 cggcacgtcg gcgaaccccg gctcgttccg cgccaccgaa ctcagcgcga aaggctggga
     1081 caccggcacg tcttggagca cgagcgccac ctaccgcctg ccgttcaaca gccgtgtcta
     1141 cgtcacgttc gccaacgcca gcctcacgct cgacagcgcc gccaactcca tcagcaacac
     1201 cgtcatcgcc gccggacaca tcggctcggc cgagctcaag gaggcgggtt tcaaaaccag
     1261 cgtcctcaac gacaaactct ttttcaccgc cgcggcctac gagcaaacgc gcgcaaacgt
     1321 cgcgagcccc accgatccca cagcgaccgt cgaggtttcc gccactcgct cccgcggttg
     1381 ggaagtggag ctgaaatggg tgccggccaa gacgttcttc ctctccgtct acgcgcgaaa
     1441 acagaaaacc atctactcgc ccaacaccgg cgcggtcatt cccgttcacg ctcgcgcgct
     1501 cggcttcatg gacgtcgtcg accccgtcac cggtgccatc atctatccgg ccgaagcatt
     1561 cctctacggc ggccgcgcgc aaatcgtgct ccccgccgac gtcaaggaat acgaggaaca
     1621 gcagggccgc cccaacaccc agctcggctt caatgccaac tacgagttcg gcggacgcta
     1681 cggcatcacg ctgagcggaa actatctttc ccgcacctac tccggccggc tcaagctcat
     1741 cgagctgccc gaatcctaca tc
//
LOCUS       DASPLT010001353         1891 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_201210, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001353 DASPLT010000000
VERSION     DASPLT010001353.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1891)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1891)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1891
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_201210"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..297)
                     /locus_tag="VEA63_13790"
     CDS             complement(<1..297)
                     /locus_tag="VEA63_13790"
                     /inference="COORDINATES: protein motif:HMM:NF013440.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GreA/GreB family elongation factor"
                     /protein_id="HYD85130.1"
                     /translation="MNHTPIYISRDDYSKLRLLLATALHSNASAALAKLRDELDRAAV
                     IDPAAFPAGVVTMNSTVEFEDLGTSEIEAYTITFPESADIDHGRISILAPIGTAL"
     gene            complement(383..1102)
                     /locus_tag="VEA63_13795"
     CDS             complement(383..1102)
                     /locus_tag="VEA63_13795"
                     /inference="COORDINATES: protein motif:HMM:NF024258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HYD85131.1"
                     /translation="MTILHVTDFHFNKRWFDWLLHRAPAHDLVVMSGDMLDLSLATPH
                     RRQIEWVTDWLNDFPRPICVCSGNHDLEWDSNAGRWTPAYWLRTLVNPRVWTDGRRVA
                     LNGMSFLNIGATTRPKGGEADVWVVHAPPSETLVATRSDGDDAGDPDLVSAVCRYAPR
                     VVLSGHVHTPLRWRELRDATLLLNPGCSAEAPFPNHILVHTAKMSCQLITVSGEDTYD
                     GEVPVNDTIDVDSAAAATVAA"
BASE COUNT          356 a          550 c          633 g          352 t
ORIGIN      
        1 gagggctgtg ccgatcggcg ccaggatcga gatgcgaccg tgatcgatgt cggcactttc
       61 gggaaacgtg atcgtgtatg cttcgatttc gctcgtgccg agatcctcga actcgaccgt
      121 cgagttcatg gtgacgacgc cggccgggaa cgcggcgggg tcgatgactg cggcgcggtc
      181 cagttcgtcg cgcagtttcg cgagcgccgc gctggcgttc gagtgaagcg ccgtggcgag
      241 cagcaatcgc agcttcgaat agtcgtcgcg ggagatatag atgggagtgt gattcatgat
      301 gattttgatt tggatggttt gggtgtgcag ccgcccacga cgctcgagaa gcgcgcgtgc
      361 cgccgcgtga aaagatcgac gatcaggccg ccacggtcgc ggccgccgcg ctgtcgacgt
      421 cgatcgtgtc gttaaccgga acttctccgt cgtaagtgtc ctcgccggag acggtgatga
      481 gttggcagct cattttcgcc gtgtgcacga gaatgtggtt cgggaatggt gcctctgcgc
      541 tgcagccagg attgagaagg agcgtcgcgt cgcgaagctc gcgccaacgt agcggcgtgt
      601 gcacatggcc cgagagcaca acacgaggcg catagcggca caccgcggaa acgagatcgg
      661 gatcgccggc gtcatcaccg tcggagcgcg tggcgacgag cgtctcgctc ggcggcgcgt
      721 gcacgaccca cacgtcggcc tcgccaccct tcggccgcgt ggtggcgccg atgttgagaa
      781 aagacatgcc gttcagcgcc acacgccggc catcggtcca aacgcgaggg ttgacgagcg
      841 tccgcagcca atacgccgga gtccagcggc cggcgttgga atcccattcg agatcgtgat
      901 tcccgctgca cacgcagatc gggcgcggaa aatcattgag ccaatcagtc acccactcga
      961 tctgccgccg gtgtggcgtc gcgagcgaca ggtcgagcat gtcgccggac atcaccacga
     1021 ggtcatgcgc cggagcacga tgaagcagcc agtcaaacca gcgcttgttg aaatggaaat
     1081 cggtgacgtg aaggatggtc atgagaattg cccaggctgc gccccgtcgt ccgcacacgc
     1141 tcagcgtgcg ggcggggaaa ggtttcgtaa gtgccgcgcg gatgacgcgg cgtgtgggtg
     1201 cattcgcgcg cgcatcccgt caggatgcgc cgcggcaagg aggaagcccg atcgtgttcg
     1261 ccccggtgcc gcgctttggg tctgagcgcg cgtttgcgcg ccgaccgtca gctcgcggcg
     1321 ccgggccgct cagggaagat atacaacgca gcgcccgccg ccatgtgcag gcggacgcag
     1381 aaaattaatt tctgatgcgt gttggggttc accgagggga cctgtgcgtc agaaaaaaat
     1441 cttgtcaagc ttcatggcga cttattgggc ggccaacgct gatatcacgc gcgtcgcgtc
     1501 atgaaatttc tgatacaggt caagagggcc atgcgtgttg gtcatggatt ggtggcgctc
     1561 agacggcgtg cacggctact ggcagctatg agcaggcgaa ccgaagcctg aagaacttga
     1621 catagctgac ggcgtggccg ttggtgccgc acgcaagtca aacccatgat ctcgttttcc
     1681 acagttcccc tgcaatcgat ctatacgacg attgtcatct gcccggcggt gccggcactg
     1741 tgagctaacg agcgcctcta gaatccgaca ggtccggccc gtttagttcg caagtgccgg
     1801 tgatcgccga aaccgctcgg ctccttaaga agcccgagcc tccatcgggc gcgttctgcg
     1861 atacgttgga gtgccggtgt atccggcgcc g
//
LOCUS       DASPLT010001354         1363 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_220538, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001354 DASPLT010000000
VERSION     DASPLT010001354.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1363)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1363)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1363
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_220538"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..283)
                     /locus_tag="VEA63_13800"
     CDS             complement(<1..283)
                     /locus_tag="VEA63_13800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD85132.1"
                     /translation="MNICLSTLASSPRRSVGAFLAVALLALTITPIAPAAVVWKNVQL
                     GGFFSQGYLKSDHNNYPVDTEDGTFDFREYALNASTTLGAHFRVGGQIFA"
     gene            complement(589..1002)
                     /locus_tag="VEA63_13805"
     CDS             complement(589..1002)
                     /locus_tag="VEA63_13805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD85133.1"
                     /translation="MKNRFVRLVFACVALGLSATLQAAPALAAHAGLKGQTLDAEGVK
                     AVLLGKRVTIGDARVVIVIAKAGDAQEAFLKANVAMTTSQFQNHWRRLFMTGGGSAPK
                     VVETEADAVKLAAETPGAIVIGDSAALGDLATLAN"
     gene            complement(1022..>1363)
                     /locus_tag="VEA63_13810"
     CDS             complement(1022..>1363)
                     /locus_tag="VEA63_13810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD85134.1"
                     /translation="DADFVLVGFGPTSIRTSKILYGYVSASRRVNTWLELGTYVSYSQ
                     DRQATNPLLRQADYALSAKFDLNEKLIFKIEGHYMNGAGKIFDTAAHPQPLATLDKEW
                     MLLAAKMTYSF"
BASE COUNT          242 a          356 c          507 g          258 t
ORIGIN      
        1 gggcgaaaat ctggccgccg acgcggaaat gcgcgccgag cgtggtggag gcgttgagcg
       61 cgtattcgcg gaagtcgaac gtgccatcct cggtgtcgac cgggtagtta ttatggtcgg
      121 acttcaaata gccctggctg aaaaagccgc cgagctggac gtttttccac accacggccg
      181 caggggcgat gggggtgatc gtcagcgcga gcagcgcgac ggcgagaaat gcgccgacgc
      241 tgcggcgcgg ggagcttgcg agagtggaga ggcaaatgtt cacgaggttg atgaggactg
      301 atggttgctg gtgtcgcgga tgagcggtgt tgataggtgc gctgagctgg tcgtccttgc
      361 atcggtgcga aaagccgttt cgtggagtca cgattttgtc acaattcacg gaagcggctg
      421 gccgttcgcg gcgaacgcgg tggaacgcag acgaaggaca atgacttcgg cgcgcgctgg
      481 tcggaggcgc gcgagggtag ctcacgcgaa aggccggtcg cgacgatgcg cggccggcct
      541 tctttgtggc gcgacctgga aagggccagc gcgtgtggcg aatgcgcttt agttcgcgag
      601 cgtcgcgagg tcgccgaggg cggcggagtc gccgatgacg atggcgccgg gcgtttcggc
      661 agcgagcttc acggcgtcgg cctcggtctc gacgaccttg ggcgcggagc cgccgccggt
      721 catgaaaaga cggcgccagt ggttttgaaa ctggctggtc gtcatggcga cgttggcttt
      781 gaggaacgct tcctgggcgt cgccggcttt cgcgataacg ataacgacgc gggcgtcgcc
      841 gatcgtcacg cgtttaccga gaaggacggc tttgacgcct tcggcatcga gcgtctggcc
      901 tttcaatccc gcgtgagcgg cgagcgccgg cgcggcttga agggtcgcgg agagtccgag
      961 ggctacacac gcgaaaacaa gacggacaaa acggtttttc atggtggtgt cgggcgatcg
     1021 gttagaacga gtaggtcatc ttggcggcga gcagcatcca ctccttgtcg agcgtcgcca
     1081 gcggctgcgg gtgcgccgcg gtgtcgaaga tctttccggc gccgttcatg tagtggcctt
     1141 cgatcttgaa gatcagtttc tcgttgagat cgaacttggc gctcaacgcg tagtcagcct
     1201 ggcgaagcag cgggttcgtg gcctggcggt cctgcgaata gctgacgtag gtgccgagtt
     1261 cgagccaagt gttgacgcgg cggctggcgg agacgtagcc gtagaggatc ttcgaagtgc
     1321 ggatactggt cggcccaaag ccgacgagga cgaagtcggc gtc
//
LOCUS       DASPLT010001355         1234 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_97308, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001355 DASPLT010000000
VERSION     DASPLT010001355.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1234)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1234)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1234
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_97308"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(135..719)
                     /locus_tag="VEA63_13815"
     CDS             complement(135..719)
                     /locus_tag="VEA63_13815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HYD85135.1"
                     /translation="MLPSPSDAAIFAIERPHPQLLKYYLLSSAVIPPLFPFIAVPMWF
                     RYHTMRYRFTDEGISMSWGILFRREVIIQYARIQDIHLRSNVVERWLGLARVLVQTAS
                     GNASAEMTLEGLLEYEAVRDFLYARMRGVKDRAHSPASAASTHAHATATMSPATHDEL
                     TATLREVAGELRQLRESLASSRTSSPSSKEAGHV"
     gene            complement(977..>1234)
                     /locus_tag="VEA63_13820"
     CDS             complement(977..>1234)
                     /locus_tag="VEA63_13820"
                     /inference="COORDINATES: protein motif:HMM:NF014171.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA C-terminal domain-containing protein"
                     /protein_id="HYD85136.1"
                     /translation="QAMVVPAWSRAAVKTLGEIAPAQTFGVQIVGLNRNGARMLNPSA
                     DETLGVGDEVLTLGTPEQLREFKVWLRERPEEEAGDADENS"
BASE COUNT          238 a          374 c          407 g          215 t
ORIGIN      
        1 cgctccagcc gagcaaacga agtttatagt agttgcgcgc cgcacgaaac acgcgggcgg
       61 agccgggcgc gccgagcggc ggctgcggat gaggaggcac gcgcaacgct gagaagaggc
      121 gggtgcggaa agaatcaaac atgaccggct tccttcgacg agggtgagga ggtgcgggac
      181 gaggcgagcg actcgcgcag ttgacgaagt tcgccggcga cttcgcggag cgtggcggtg
      241 agttcgtcgt gcgtggccgg cgacatggtc gcggtcgcgt gcgcgtgcgt cgaagcagcg
      301 gatgccggcg aatgagcgcg gtctttcacg ccgcgcatcc gtgcatagag gaaatcgcgg
      361 accgcttcgt attcgagcag cccctcgagc gtcatctcgg cggacgcgtt gccgctcgcc
      421 gtttgcacga gcacacgggc gagtccgagc cagcgctcaa ccacgttcga gcggagatga
      481 atgtcctgaa tgcgcgcgta ttggatgatc acttctcggc ggaatagaat tccccagctc
      541 atcgaaattc cctcgtcggt gaagcggtag cgcatcgtgt gatagcgaaa ccacatcggg
      601 acggcgatga aggggaacag tggcggtata acggcgctgc tgagcaggta gtattttagg
      661 agctgcggat gcggccgctc gatcgcgaag atcgcagcgt cggaggggct ggggagcatg
      721 agggcatgac acgcgagacc accgttttgt cgcaagcttg cgctgcgaca tcgtgctctc
      781 gcgccgaagg agaagaaagc accgccgcag ctacagcggt tcaaatggaa tcctagcccg
      841 gaaaattcac acctgtagcg aaacgcgtga gcgtttcgtc tctcgcaaca gactgcgcgc
      901 acctcgcatc tgcgtgtgac aaacgcggct aaacccgccg tgagtgtcac aacttcacgg
      961 atacgccggc accttcctat gagttctcgt ccgcgtcgcc ggcctcttcc tccggacgct
     1021 cgcgcagcca gaccttgaat tcgcgcagct gctcgggtgt gcccagcgtc agcacttcgt
     1081 cgccgacgcc gagcgtttcg tcagcgctgg gattcagcat ccgcgcgccg ttccggttta
     1141 accccacaat ctgcacgccg aaggtttgcg cgggcgcgat ctcgccgagt gttttcaccg
     1201 ccgcgcggct ccaagccggc accaccatcg cctg
//
LOCUS       DASPLT010001356         1602 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_76683, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001356 DASPLT010000000
VERSION     DASPLT010001356.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1602)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1602)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1602
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_76683"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1602)
                     /locus_tag="VEA63_13825"
     CDS             complement(<1..>1602)
                     /locus_tag="VEA63_13825"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HYD85137.1"
                     /translation="AHLETVAGLTHNFFAALNVSPLAGQLWPAAADEMLAKNLGVIIS
                     EALWERRFNRDPNLVGSMIVVNGVTLPVAAVVSTRDIPAHFIGDSDLWIALSDSGDVG
                     NPWALTEVSRGAAWLRSWGTLRPGVALDQATANLGAVSSLIAQEHPSVHERGQLSATS
                     LPRYLLRYDRATVVVSLTVLAIILATFLVAISNASLLASLQIFRRAVDFKIKSALGAS
                     RRQLQAEVAIVCAILGTVLVLASSTLSFLLLKALPWLGATALLPHDRSNLDLAPTLIA
                     TTMVVAACVALWLWVVQALALTTIKAETLVRDARHQGLSASISTRFLLLQGALSITLM
                     FPAAIHLKSSRNTERLPLGFPVENLLIVDLDLRDRGHNPKTGVTYLRRLYAEFGNSPR
                     FTAIGVAQRPPLEPLPLTKPFVGGERSSTSVGYSVIGPGYFDGLGVKLLGGRDFDFKD
                     DNSKDRVVIINEAMAQKFWPGDNAVGRDVDLFPGSKSRIIGVVQNFRSDLLQDRQPVA
                     FIPLTLAWSSRLSLYLRFNGPPAAAA"
BASE COUNT          326 a          427 c          602 g          247 t
ORIGIN      
        1 gcagcagcgg cgggcgggcc gttgaatcgc agatacagac tcaggcgcga ggaccacgcg
       61 agcgtcagcg ggatgaaggc gacgggctgc cggtcctgca ggagatcgga gcggaagttt
      121 tggacgacac cgatgatcct ggacttggag ccagggaaga ggtcgacgtc gcggccgacc
      181 gcattatcgc cgggccaaaa cttctgggcc atggcttcgt tgatgatgac gacacggtcc
      241 ttggagttgt cgtccttgaa atcgaaatcg cgtccgccga ggagtttcac gccgagaccg
      301 tcgaagtagc cggggccgat gacggaataa ccgaccgacg tgctggagcg ttcaccgccg
      361 acgaacggtt tcgtgagcgg gagtggttcg aggggcggac gctgtgcgac gccgatggcg
      421 gtgaagcgcg gggagtttcc aaactcagcg tagagccgcc gcaggtaagt gacgccggtc
      481 tttggattgt ggccgcgatc gcgaagatcg aggtcgacga tgagcagatt ctcgaccgga
      541 aagccgagcg gcaggcgctc ggtgttgcgg ctggatttga gatgaatcgc cgcgggaaac
      601 atgagcgtga tcgagagcgc gccttgcagc agaaggaaac gcgtgctgat cgaagcggag
      661 agaccttggt ggcgcgcatc gcggacgagg gtttcggcct tgatcgtggt gagagcgagc
      721 gcttgcacga cccagagcca cagggcgacg caggcggcga cgaccatcgt ggtcgcgatc
      781 agcgtcggcg cgagatcgag attgctgcga tcgtgcggca acagggcggt tgcgcccagc
      841 cagggcaggg ctttcagcag caggaaggaa agcgtcgagc tggcgagcac cagcacggtg
      901 ccgaggattg cgcagacgat ggcgacttcc gcctgaagtt gccggcggct ggcgccgagg
      961 gcggacttga tcttgaagtc gacggctcgg cgaaagatct gcagcgaagc cagcagcgag
     1021 gcattgctga tcgcgacgag aaaggtggcc aggatgatgg cgaggaccgt gagcgagacg
     1081 accacggtcg cgcgatcgta acggaggaga tagcgaggga gagaggtggc ggagagttgt
     1141 ccccgttcgt ggacggaggg atgttcctgc gcgatgagac tgctgacggc gccgaggttg
     1201 gccgtcgctt gatcgagagc gaccccaggg cgcaacgtgc cccagctcct gagccaggcg
     1261 gcgccgcggc tgacctcggt caaagcccat ggattgccca catcgccgga gtcggagagt
     1321 gcgatccaca aatcgctgtc gccgatgaaa tgcgcgggaa tatcgcgcgt cgacacgacg
     1381 gccgcgacgg ggagcgtgac gccgttcacc acgatcatcg aaccgacgag attgggatcg
     1441 cggttaaagc gacgttccca gagagcctcg ctgatgatga cgccgaggtt tttggccagc
     1501 atctcgtccg cggcggccgg ccagagctgg cccgcgaggg gcgagacgtt gagcgcggcg
     1561 aaaaaattat gagtgagacc ggcgacggtt tcgaggtggg cg
//
LOCUS       DASPLT010001357         1817 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_76725, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001357 DASPLT010000000
VERSION     DASPLT010001357.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1817)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1817)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1817
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_76725"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..394)
                     /locus_tag="VEA63_13830"
     CDS             complement(<1..394)
                     /locus_tag="VEA63_13830"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HYD85138.1"
                     /translation="MLNAASASHDPSTAAVPVLPSGAALDSSILRVLMDTIPDRIYFK
                     DLQSRFVRVNAAYAKWHGIASPEFVIGKTDHDLFAAVHADVALAQEQEIIRTGEPMIA
                     KVEKLTLKDGSVAWGSATKMAWRDASGRI"
     gene            complement(564..1667)
                     /gene="ychF"
                     /locus_tag="VEA63_13835"
     CDS             complement(564..1667)
                     /gene="ychF"
                     /locus_tag="VEA63_13835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007358980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HYD85139.1"
                     /translation="MLKAGIVGLPNVGKSTLFNALTRSRKAEAANYPFCTIDPNVGVV
                     IVPDERAYVLQKIAKTNVVVPAAIEFVDIAGLVAGASKGEGLGNQFLANIREVDAIVQ
                     VVRCFEDPDVVHTMGAVDPVRDIEVITTELVLADLDAVSKRIEKTQKKAKSGDKEAIA
                     EVALLQKLEPHLNSGKTANTLPATDEEKAAMKLLQLLTAKPVLFACNVAESDLATAEQ
                     NPFVQKVAEYVRTHHDAAYVPISAKIEAELIDLPPEDAKAFLKDLGVDDSGVSSLIKG
                     TYGLLGLQTYFTAGEKEVRAWTIKKGWKAPQAAGVIHTDFEKGFIKAEIVSYDDLTKL
                     GSVAAAREAGKYRLEGKEYIFQDGDVALFRFNN"
BASE COUNT          315 a          502 c          640 g          360 t
ORIGIN      
        1 tgatccggcc gctagcgtcg cgccacgcca ttttggtggc ggagccccag gcgacgctgc
       61 cgtcctttag cgtgagtttc tcgaccttcg cgatcatcgg ctcgccggtg cggatgattt
      121 cctgctcctg cgcgagtgcg acgtcggcat ggaccgcggc gaaaagatcg tgatcggtct
      181 tgccgatgac aaactcgggt gaggcgatgc cgtgccattt ggcgtaggcg gcgttgacgc
      241 ggacgaagcg gctctggaga tccttgaagt agatccggtc cggtatcgta tccatcaata
      301 cgcgcaggat cgacgaatcg agcgcggcgc cgctgggcag caccgggacg gcggcggttg
      361 agggatcgtg ggaggcggac gcggcgttga gcacaggaga acggcggcga agcgatgacg
      421 attcgccgag gagaacgtcg cggagaaaaa ccaagcgcgc cgcgtttagc aatgctcggt
      481 gtcgacggac agaccgaccg tcggcggtgc gacctctgcc gcaggccgac aggcacaaag
      541 gcctgccgct acacgggccg gggttagttg ttgaaacgga atagcgcgac gtcgccgtcc
      601 tggaaaatgt attccttgcc ttcgagccga tacttgccgg cttcgcgcgc ggcggcgacg
      661 ctgccgagct tcgtgagatc gtcgtaagaa acgatttccg ccttgatgaa gcctttttcg
      721 aaatcggtgt gaatgacgcc cgccgcctgc ggtgctttcc agcccttttt gatcgtccag
      781 gcgcgcacct ctttttcacc ggcggtgaaa tacgtctgca aaccgagcaa tccgtaggtg
      841 cccttgatga gcgaggaaac gccggagtcg tcgacaccga gatctttcaa aaacgccttc
      901 gcatcttccg gcgggagatc gatgagctcg gcctcgatct tcgcgctgat cggcacgtag
      961 gcggcgtcgt ggtgggtgcg cacgtattcc gcgacttttt ggacgaacgg attttgttcg
     1021 gcggtcgcga ggtcgctctc ggcgacgttg caggcgaaga gcacgggctt cgcggtgaga
     1081 agctgcagga gcttcatcgc ggctttttcc tcgtcggtcg cggggagggt gttggcggtc
     1141 ttgccggagt tgagatgggg ttcgagtttt tgcagcaacg cgacttcggc gatcgcttct
     1201 ttgtcacccg acttcgcctt cttttgcgtt ttctcgatgc gcttcgaaac ggcgtcgagg
     1261 tcggcgagga cgagctcggt ggtaatgacc tcgatgtcgc gcacgggatc gacggcgccc
     1321 atggtgtgca cgacgtcggg atcctcgaag cagcggacga cctgcacgat ggcgtcgacc
     1381 tcgcggatgt tggcgaggaa ctgattgccg agaccttcgc ccttgctggc gccggcgacg
     1441 agaccggcga tgtcgacgaa ctcgatggcg gcggggacga ccacgttggt cttggcgatc
     1501 ttttgcagaa cgtaggcgcg ttcgtcaggc acgatcacga cgccgacgtt gggatcgatg
     1561 gtgcaaaacg gatagttcgc ggcctcggcc ttgcgggagc gggtgagcgc gttgaagagg
     1621 gtggatttgc cgacgttggg caggccgacg atgccagctt tgagcatagg ggcggacgtt
     1681 ctcagttggg cgggcgaacg gtcaacagaa aaggccgcag cggggattcg tgtagccgca
     1741 gcggtttcgg tgcgggaccg catgccaacg catgcggcta cgggcacgcg gcaaggcgcg
     1801 ggatgggact gccgcgg
//
LOCUS       DASPLT010001358         1222 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_55588, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001358 DASPLT010000000
VERSION     DASPLT010001358.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1222)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1222)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1222
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_55588"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..340)
                     /locus_tag="VEA63_13840"
     CDS             complement(<1..340)
                     /locus_tag="VEA63_13840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007362886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HYD85140.1"
                     /translation="MPASPIPVALQLWSVREDMKCDFAATVAEVARIGYAGVELAGYG
                     NLDAKGAKGALDAAGLKVAGMHVNITSLRTSPETIIADALLFGTRHVTISFWSPEQFV
                     SVAAVEKIAEE"
     gene            complement(546..>1222)
                     /locus_tag="VEA63_13845"
     CDS             complement(546..>1222)
                     /locus_tag="VEA63_13845"
                     /inference="COORDINATES: protein motif:HMM:NF024491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HYD85141.1"
                     /translation="TQVRVEGPAVRQMQAIFAVNWMQTTGKLLLGDDYFPRFEAAGRT
                     PAQCFKSGPGEGAESARMSYILAIASAREAIDIEHAYFIPDDLATQMLLEARERNVRV
                     RVIVPAQNDSRFGRAASRSRWGALLAAGVEFYLYEPALLHSKTMSVDDTFVTVGSVNF
                     DNRSFSINDEIALNVIDPSIGRAHRRMFEEDLAKSRPYTLEEHNARPAYIKVLDKFCG
                     LFRSQL"
BASE COUNT          220 a          328 c          458 g          216 t
ORIGIN      
        1 gctcctcggc gatcttctcg acggcggcga ccgagacgaa ttgctcgggc gaccagaacg
       61 aaatggtgac gtgacgcgtg ccgaagagga gcgcgtcggc gatgatggtt tccgggctgg
      121 tgcggagcga ggtgatgttg acgtgcatgc ccgcgacttt gaggccagcg gcatcgagcg
      181 cgcctttggc gcccttcgca tcgagattgc cgtagccggc gagttccacg ccggcgtagc
      241 cgatgcgcgc gacttcggcg acggtcgcgg cgaagtcgca tttcatgtct tcgcggacgg
      301 accagagttg gagggcgacg gggatggggc tcgcgggcat ggaaagggag tgagggagta
      361 agggaatggg agtgggatga gtatcgcgag gatgcgcgcg gcgggcgtca acgcggaagg
      421 tgaatagggt tcgcgatgaa gatgcacggc agcgccgggc gggtagcgaa aagcgtgagc
      481 ttttcgtggt tcggcgaaaa ccggcggacg aaacgctcac gcgttttgct accgtgcggt
      541 gacgtctaca actgcgagcg gaagaggccg cagaatttat cgagaacctt gatgtaggcc
      601 gggcgcgcgt tgtgttcttc cagcgtgtag ggccgcgatt tcgcgaggtc ttcttcgaac
      661 atgcggcgat gcgcgcggcc gatggacgga tcgatgacgt tgagcgcgat ctcgtcgttg
      721 atgctgaagg agcggttgtc gaaattcacg gagccgaccg ttacgaacgt gtcatcgacg
      781 ctcatggttt tgctgtgcag gagcgcgggc tcgtagaggt aaaactcgac gccggcggcg
      841 agcagggcac cccagcgcga gcgggacgcg gcgcggccga agcgcgagtc gttttgcgca
      901 gggacgatca cgcgcacgcg cacgttgcgt tcgcgggctt cgagcagcat ctgcgtcgcg
      961 agatcgtcgg ggatgaaata cgcgtgctcg atgtcgatcg cctcccgcgc ggacgcgatc
     1021 gcaaggatgt aggacatgcg cgcggattcg gcgccttcgc cggggccgct cttgaaacat
     1081 tgcgcgggcg tgcggccggc ggcttcgaag cgcggaaaat aatcgtcgcc gagcaggagc
     1141 ttgccggtgg tctgcatcca gttcaccgcg aagatcgcct gcatctgacg aacggcggga
     1201 ccttcgacgc gcacctgcgt cc
//
LOCUS       DASPLT010001359         1195 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_220617, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001359 DASPLT010000000
VERSION     DASPLT010001359.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1195)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1195)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1195
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_220617"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1195)
                     /locus_tag="VEA63_13850"
     CDS             complement(<1..>1195)
                     /locus_tag="VEA63_13850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD85142.1"
                     /translation="AKTPPLAAEISGDAPAPAPAVNQDVIAAWLVANGFQPATPAATD
                     VDLLGTTSENTDLLLAADEVSNSPVVTGDSIATSGRGSGVSVPFVASDHAPRAVARAY
                     TFTSPRLQTPNTENISEEPVAGEALAGEALAGEQPAATTARSLPSDNAAAAARHLSTQ
                     QQPHAAAVSDSRAMIPRAASLRDDSSLLPADVVAAETDATLDEAANRAPIAVAISPEL
                     TRAQGRAFAAHVQPTLPTPAKEAGTSSSLAPVDAAGTAENFAVQPETSRNDDSRQRGK
                     GEKHSLSINAEEVTMRSSGFGINAAKTETLMSALATPAASSPPVAGIEHLSPAPAVFD
                     ALVNQAGHEATAMAQAARRSVDSALAVAEQFATGDKRAVTLQFSVSGVDLGVHVELRG
                     DGVHTT"
BASE COUNT          214 a          356 c          411 g          214 t
ORIGIN      
        1 ggtcgtgtgg acgccgtctc cgcgaagctc gacgtgcacg ccgagatcca cgccggaaac
       61 cgaaaactgc agcgtcaccg cgcgcttgtc gccggtcgcg aattgctcgg ccaccgcgag
      121 ggcggaatca accgaccgcc gcgccgcttg cgccatcgcg gtcgcttcgt ggcccgcttg
      181 attcaccagc gcgtcgaaca ccgccggtgc tggcgacaaa tgctcgatcc cggccacggg
      241 tggcgaagaa gctgcgggag tagcgagtgc ggacatgaga gtctcggttt tagcagcgtt
      301 gatgccaaag cctgatgaac gcatcgtaac ttcctctgcg ttaatcgata gagaatgttt
      361 ttctcctttt ccgcgctgcc gtgaatcatc gttccgcgaa gtctcgggct gaacggcaaa
      421 attttccgcg gtgccggcgg catcgaccgg ggccaacgaa gatgacgtcc cagcttcctt
      481 tgccggcgtc ggtaaagtgg gctgaacgtg cgcggcgaaa gcacggcctt gcgctctcgt
      541 gagttcgggc gagatcgcga cagcgatcgg cgcgcggttg gctgcttcgt cgagcgtcgc
      601 atcggtttcg gcggcgacga cgtcggccgg gaggagcgac gagtcgtcgc gaagcgaagc
      661 cgcgcgcggg atcatcgcgc gagaatctga aacggcagcg gcgtgcggct gttgctgtgt
      721 agaaagatgg cgcgcggcgg cggcggcgtt atccgagggc aacgacctgg cggtcgtggc
      781 ggcgggctgc tcgccggcta aagcttcgcc ggctaaagct tcgcctgcga ccggctcctc
      841 ggaaatattc tccgtatttg gagtttggag acgcggcgac gtaaatgtgt aagcacgcgc
      901 gacggcacgc ggcgcatgat cggaagcaac gaacggaaca gaaaccccac ttccccgccc
      961 tgacgtcgca atgctgtcgc ccgtaacgac tggcgaattc gacacctcgt cggctgcgag
     1021 gagcagatcg gtgttttcgc tcgtggtgcc gagaagatcg acatcggtcg cggcgggcgt
     1081 cgcgggttgg aaaccattcg cgacgagcca ggctgcgata acatcctggt taacggcggg
     1141 agcaggagcc ggcgcatcgc cgctgatttc agccgcgagc ggcggggttt tcgcg
//
LOCUS       DASPLT010001360         1963 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_40428, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001360 DASPLT010000000
VERSION     DASPLT010001360.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1963)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1963)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1963
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_40428"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1963)
                     /locus_tag="VEA63_13855"
     CDS             complement(<1..>1963)
                     /locus_tag="VEA63_13855"
                     /inference="COORDINATES: protein motif:HMM:NF036578.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD85143.1"
                     /translation="SWRASWIGIDEPSRANQWTAFRRSFSIERVPATAPTRIAADSKY
                     WLWVNGTLVVREGGLKRGPTPTDTYFDELDLAPHLRAGSNTIAVLVWHFGREGFSHKN
                     SGRSGLLLQMDTPTAEAPLLVTDENWQARVHPAFGDTGEPHPNMRLPEANIRFDASRD
                     ISGWTKGNVAHETWPRAVALGRAPCAPWNRLVRRPIPLWRDYGMRAYVDGPALPSVAD
                     GAVIKTRLPYNAQVTPYLEIDAPAGLTIDIRTDNYLGGSEANVRAEYVTRAGRQEFEC
                     YGWMNGHEIHYSIPAGVKVLKLAYRETGFDADLGAGTFSCDDPFLQRLREKAVRTLYV
                     TMRDTYMDCPDRERAQWWGDAVNELGEVFYTFDRGADSLTRKAILELIGWQKPDGVLY
                     SPVPAGNWERDLPLQSLASISRYGFWTYAFYSGDFETLRAVYPGVKRYLEIWDFASSG
                     LVVARPGAWSWGDWGENVDMTVLANAWYYLALQGQRHMAVALGHAGDLPWIDARLAAI
                     EAAFNRTFWNGREYRSPDHKGLTDDRANALAVVAGLALREQYPALLDVFRQHEHASPY
                     MEKYVLEALCLMDQPSFAQDRIKRRYAKMVEHDYSTLWEGWGIGAEGYGGGTINHAWS
                     GGPLTIMSQYFAGIAPTSPAFATYHVLPQP"
BASE COUNT          363 a          598 c          645 g          357 t
ORIGIN      
        1 ggttgcggga gcacgtgata cgtggcgaac gcgggcgagg tcggtgcgat gccggcgaag
       61 tattgcgaca tgatcgtgag cggcccgccg ctccacgcgt gattgatcgt gccgccgccg
      121 tagccttcgg cgccgatgcc ccagccttcc cacagcgtgc tgtagtcgtg ctcgaccatc
      181 ttcgcgtaac gacgcttgat ccgatcttgc gcgaaggagg gctgatccat gaggcacagc
      241 gcctcaagaa cgtatttctc catgtaagga ctcgcgtgtt catgctggcg gaaaacgtcg
      301 agcaacgccg gatactgctc gcgtagtgcc aaaccggcta caacggcgag cgcgttggcg
      361 cggtcgtcgg tgagcccctt gtgatcgggc gagcggtatt cgcgtccgtt ccagaaagtg
      421 cgattgaaag ccgcttcgat ggccgcgagt cgcgcgtcga tccacggcag atcgccggcg
      481 tggccgagcg cgacggccat gtgccgctgg ccttgcagcg cgaggtaata ccaggcgttg
      541 gcgagcacgg tcatgtcgac gttctcgccc caatcgcccc acgaccacgc gccgggtctc
      601 gcgactacga ggccgctcga tgcgaaatcc cagatttcga gatagcgttt cacgccggga
      661 taaacggcgc gcaaggtttc gaaatcgccg ctataaaaag catacgtcca gaaaccgtag
      721 cggctgatcg atgcgagcga ttgcagtggc aaatcacgct cccaatttcc cgcgggcacg
      781 ggcgagtaga gaacgccgtc cggcttctgc cagccgatca gctcgaggat cgccttccgc
      841 gtgagcgaat cggcaccgcg gtcgaaggtg tagaaaacct cgccgagctc gttgacggcg
      901 tcgccccacc actgcgcgcg ctcgcggtcg gggcaatcca tgtaggtgtc gcgcatggtc
      961 acgtagagcg tgcgcacggc cttctcgcgc agacgctgca gaaacggatc gtcgcacgag
     1021 aaagtcccgg cgccgagatc ggcgtcgaac ccggtttccc gatacgcgag cttgagcacc
     1081 tttacgcccg caggaatcga gtagtggatc tcgtgcccgt tcatccagcc gtagcattcg
     1141 aactcctgcc ggcctgcacg cgtgacgtat tcggcgcgaa cgttggcctc gctgccgcca
     1201 agataattgt cggtgcggat gtcgatcgtg aggccggcag gcgcgtcgat ctcgaggtag
     1261 ggcgtgactt gcgcgttgta aggcaaacga gtctttatga ccgcgccgtc agccaccgac
     1321 ggtagcgctg gaccatcgac gtaggcgcgc atgccgtagt cgcgccagag cggaatcggt
     1381 cgccgcacga ggcggttcca cggagcgcag ggcgcgcgac cgagtgcgac ggcgcggggc
     1441 caggtttcgt gagcgacatt tccctttgtc cacccggaga tatcgcgact ggcgtcgaaa
     1501 cggatattcg cttccgggag ccgcatgttg ggatgcggtt cgccggtgtc tccaaaagcc
     1561 gggtgcaccc gggcctgcca gttctcgtcg gtcacaagaa gcggtgcctc agccgtgggt
     1621 gtatccatct ggagaagcaa tccgctgcgg ccggagtttt tgtgactgaa gccttcgcgt
     1681 ccgaaatgcc agacgagcac ggcgattgtg ttcgagccgg cgcgcaagtg cggtgcgaga
     1741 tcgagctcgt cgaaatacgt gtcggtcggc gtcgggccgc gtttcaatcc accctcgcgc
     1801 acgaccaacg tgccgttgac ccagagccaa tatttcgagt cggcggcaat gcgggtaggc
     1861 gcggtggccg gaacacgctc aattgagaaa ctgcggcgaa atgccgtcca ttgattcgct
     1921 cgcgagggtt catcgatgcc gatccacgat gcgcgccagg aga
//
LOCUS       DASPLT010001361         1292 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_76775, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001361 DASPLT010000000
VERSION     DASPLT010001361.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1292)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1292)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1292
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_76775"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1271)
                     /locus_tag="VEA63_13860"
     CDS             complement(<1..1271)
                     /locus_tag="VEA63_13860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD85144.1"
                     /translation="MASFQRSLAEKPGVAELWLGLAFAQLQAGVPGEAKSSAQRAQAL
                     DGKLRDVGKVLQHAEAALRTGNASQEFLSRAALTWPQLMEHAVTRQKLRTSLHERAAL
                     HRLERERRRLLERPVWCVDKQGKPAHTDQKGESAQSSANDSASWSVRPPWPDEMPRIQ
                     AQFAPALRQTRATRHSIWVLAAEATERLAGVIVLSGVEDAGAKTVGDGPAARADLDLR
                     EAWIETPAGDALLDVVIRHAATIGLVSLDLQARLSAPMNALLQRHGFKEVIRHEVWTA
                     SMPDAIASQHAEYGRILKRWPVPIEPFCPTHLEAARQICAGTGLLAPEKVVLKSLRHP
                     RGIDPATSFVAGSADHPIAILLSGIDGSTAEIEIVARNPATLMTAPAAVPALLLRFLF
                     AAQNLGCREVRCSMRPEVTPSLITLMKKWNGR"
BASE COUNT          216 a          395 c          424 g          257 t
ORIGIN      
        1 cgaccgttcc attttttcat cagtgttatc agcgaaggtg tcacttcagg acgcatggaa
       61 caccgcactt cgcggcagcc gaggttctgc gcggcgaaga ggaagcgcaa gaggagcgcg
      121 ggcacggctg ccggcgcggt catcaacgtc gccggatttc tcgcgacgat ttcaatctcg
      181 gcggtcgatc cgtcgattcc actgagcaga atggcgatgg ggtggtcggc tgagccggct
      241 acgaaggagg tcgccgggtc gattccgcgc ggatgtcgca gcgatttcaa cacgaccttt
      301 tcgggcgcga ggaggccggt gccggcacag atttggcgcg cggcttccaa atgggtggga
      361 caaaacggtt cgatcggcac cggccagcgt tttaagatcc gaccatactc cgcgtgttgg
      421 ctggcaatcg cgtcgggcat cgatgccgtc cagacttcgt ggcggatgac ttccttgaat
      481 ccgtgccgct gcagcaaggc gttcatcggt gcgctcaggc gcgcttgaag gtcgagtgag
      541 accaggccga tcgtggcggc gtggcgtatc accacatcga gcaacgcatc gcccgccggc
      601 gtttcgatcc acgcttcgcg cagatcaaga tcggcgcgag cggccgggcc gtcgccgact
      661 gttttggcgc ccgcatcttc gactccgctc agaacaatga cgcctgccag ccgttcggtg
      721 gcctcggccg cgagcaccca gatggaatgc cgggttgcgc gggtttgacg aagcgccggg
      781 gcaaattgcg cctgaatcct cggcatttca tccggccagg gtggccgcac cgaccacgag
      841 gcggagtcgt tcgccgacga ctgggccgac tcgccctttt ggtcggtgtg ggcgggcttg
      901 ccctgtttgt cgacgcacca cacgggtcgt tccaacaaac gtcttcgttc gcgctccagt
      961 cgatgaagcg cggcccgctc atgcagcgac gttcgtaatt tctgccgtgt gacggcgtgc
     1021 tccattaatt gcggccaagt cagggcggcg cggctcaaaa attcttggga tgcgtttccg
     1081 gttcgcagcg ccgcttcggc gtgctgcagc accttcccca catcgcgcaa ttttccgtcg
     1141 agagcttggg cacgttgggc ggatgatttt gcttcgccag gcacacccgc ttgcaactgc
     1201 gcgaatgcca ggcccagcca gagttcggcg acgccgggct tttctgcgag cgagcgttgg
     1261 aaacttgcca cgctgtcgga tggcggacga cg
//
LOCUS       DASPLT010001362         1501 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_159120, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001362 DASPLT010000000
VERSION     DASPLT010001362.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1501)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1501)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1501
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_159120"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            107..>1501
                     /gene="leuS"
                     /locus_tag="VEA63_13865"
     CDS             107..>1501
                     /gene="leuS"
                     /locus_tag="VEA63_13865"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HYD85145.1"
                     /translation="MATSSKDYNFQEIEPHWQTFWEKNHSARADNDSSKPKFYVLDMF
                     PYPSGAGLHIGHPEGYTATDIMARYMRARGYNVLHPIGWDAFGLPAEQHAVKTGTHPA
                     ANTQNNIVNFRRQIKALGFSYDWDREVDTTDPKYFRWTQWIFLQLFKRGLAYVDERPV
                     WWCPELRTVLANEEIVDGKSEVGGFPVERRNLRQWVLRITAYADRLLSDLKDVDWPES
                     TKRMQEAWIGRSEGAEIEFALEDKALGTLKVFTTRPDTLFGCTYMVVAPEHPLVETLT
                     TPEHRHAVDSYKKRAAAKSDLERTDLAKDKSGVFSGSYAINPVNNARVPIWIADYVLM
                     GYGTGAIMAVPAHDDRDFEFATQYELPIVQVIANDDAEGILPYTGEGHLINSPGYDGL
                     RWSEAKKRITAELASRGIGKATVNFKLRDWLFSRQRYWGEPFPITWVNEADYRRAAAA
                     RTADVPPTPVTFTSE"
BASE COUNT          314 a          522 c          414 g          251 t
ORIGIN      
        1 cggctcaccg ccgcattcgc cgccgtcagg gccgcacacc aagtcacgaa gagggtgaag
       61 attgaccggg cagggaaagg tttcacagtg gcgagttcac acgcgaatgg ctaccagcag
      121 caaagattac aacttccaag agatcgagcc gcactggcaa actttctggg agaaaaacca
      181 ctccgcccgc gcggacaacg actcgtcgaa gccgaagttt tacgtgctcg atatgttccc
      241 gtatccgtcc ggcgccggcc tgcacatcgg acatccggag ggttacaccg cgaccgacat
      301 catggcgcgt tacatgcgcg cgagaggtta caacgttctc cacccgatcg gctgggacgc
      361 ctttggtctg cccgccgaac agcacgcggt gaaaaccggc acgcatcccg ccgccaacac
      421 gcagaacaac atcgtcaact tccgccgtca gatcaaagcg ctcggcttct cctacgattg
      481 ggaccgcgaa gtcgacacga ccgaccccaa gtatttccgc tggacgcagt ggattttcct
      541 gcagctcttc aaacgcggcc tcgcttacgt cgacgagcgt cccgtgtggt ggtgcccgga
      601 gctgcgcacc gttctcgcca acgaagaaat cgtcgacggc aagagcgagg tcggcggttt
      661 tcccgtcgaa cgtcgcaatc tccgccaatg ggtgctgcgc atcaccgctt acgccgatcg
      721 cctgctctcc gatctcaaag acgtcgactg gcctgaatcc accaaacgga tgcaggaagc
      781 ctggatcggc cgcagcgagg gcgccgagat cgagttcgcg ctcgaagaca aagcgctcgg
      841 caccctcaag gtgttcacca cgcgtcccga cactcttttc ggttgcacct acatggttgt
      901 tgcgcccgag catccgctcg tcgaaacgct cacgacgccc gaacaccgcc atgcggtcga
      961 ttcctacaag aaacgcgccg ccgccaagag cgatctcgag cgcaccgatc tcgcgaagga
     1021 caagtccggc gtgttcagcg gcagctacgc gatcaatccc gtcaacaacg cccgcgttcc
     1081 catctggatc gccgactacg tgctcatggg ctacggcacc ggcgcgatca tggccgtgcc
     1141 cgcgcacgac gatcgcgact tcgaattcgc cacgcaatac gagctgccca tcgtgcaggt
     1201 catcgccaac gacgacgccg agggcattct cccttacacc ggcgagggcc atctcatcaa
     1261 ctcgccgggt tacgacggcc tgcgctggtc cgaagcgaaa aaacgcatca ccgccgaact
     1321 cgcctcacgc ggcatcggca aggccaccgt caacttcaag ctccgcgatt ggttgttctc
     1381 gcgccagcgt tactggggcg aaccgttccc gatcacctgg gtcaacgaag ccgattaccg
     1441 ccgcgccgcc gccgcgcgca cggccgacgt tccgccgact cctgtcacgt tcaccagcga
     1501 g
//
LOCUS       DASPLT010001363         1064 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_240511, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001363 DASPLT010000000
VERSION     DASPLT010001363.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1064)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1064)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1064
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_240511"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1064)
                     /locus_tag="VEA63_13870"
     CDS             complement(<1..>1064)
                     /locus_tag="VEA63_13870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HYD85146.1"
                     /translation="RFNTSLLRLRRQVGEEGKIGERLQIGDAVGSWAERIEAINSLVD
                     DLSQPTVEVGRVIGAVAKGDLSQAMPLELGGRPLKGEYLRTAKLVNGMVGQLEAFSVE
                     VIRVAREVGTEGKLGGQAQVKKVSGVWKELTESVNQMAGNLTAQVRNIAEVTIAVANG
                     DLSKKITVDVRGEILQLKEAINVMVDQLRSFAAEVTRVARDVGTEGKLGGQAVVPGVS
                     GTWKDLTDSVNAMAGNLTAQVRNIAGVTTAVARGDLSRKITVDVKGEILELKETINTM
                     VDRLNAFASEVSRVAREVGSEGKLGGQAQVPGVAGTWKDLTDNVNSMANNLTTQVRNI
                     ADVTTAVARGDLSRKITVDV"
BASE COUNT          181 a          411 c          264 g          208 t
ORIGIN      
        1 gcacgtcgac cgtgatcttg cgcgacaagt ccccgcgcgc caccgccgtc gtcacgtccg
       61 cgatgttgcg cacctgcgtc gtcaaattat tcgccatcga attcacgttg tccgtgagat
      121 ccttccacgt gcccgcgacg cccggcacct gcgcctggcc gccgagcttg ccctccgagc
      181 ccacttcgcg cgcgacacgc gacacttccg atgcaaacgc attcaaccgg tccaccatcg
      241 tgttgatggt ttccttcagc tccaaaatct cgcccttcac atcgacggtg atcttgcgcg
      301 acaagtcgcc gcgggcgacg gccgtcgtca cgcccgcgat gttgcgcacc tgcgcggtga
      361 gattgcccgc catcgcattc accgaatcag tcaaatcctt ccacgtgccc gacacgccgg
      421 gcacgacggc ctgaccgccg agcttgcctt ccgtgcccac gtcgcgcgcc acgcgcgtca
      481 cctccgccgc gaacgaccgc agttggtcga ccatgacgtt gatggcttcc ttgagctgca
      541 gaatctcgcc acgcacatcg accgtgatct tcttcgacaa gtcgccgttc gccacggcga
      601 tcgtcacctc cgcgatgttg cgcacctgcg ccgtgagatt gccggccatc tgattgacgg
      661 actcggtcaa ttccttccac accccggaca cttttttcac ctgcgcctgg ccgccgagtt
      721 tgccctccgt gccgacctcg cgcgccacac gaatcacctc gaccgaaaac gcttcaagct
      781 ggccaaccat gccgttgacc agtttcgccg tgcgcaagta ttcgcctttc aacggccgcc
      841 cgccgagctc gagcggcatc gcttgggaaa gatctccctt cgccaccgca ccgatcacgc
      901 gacccacctc gaccgtcggc tgcgaaagat catccacaag cgaattgatc gcttcgatcc
      961 gctccgccca gcttcccacc gcgtcgccga tctgtaaccg ctcgccgatc ttgccctctt
     1021 ctccgacctg ccggcgcagc cgcaacaagc tcgtattgaa acgt
//
LOCUS       DASPLT010001364         1342 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_55688, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001364 DASPLT010000000
VERSION     DASPLT010001364.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1342)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1342)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1342
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_55688"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..602
                     /locus_tag="VEA63_13875"
     CDS             <1..602
                     /locus_tag="VEA63_13875"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: protein motif:HMM:NF002298.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004640 - phosphoribosylanthranilate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HYD85147.1"
                     /translation="CGITRVEDGVAAAAAGADAIGLNFCEKSPRVIDAERARSFCAAL
                     PPFVARVGLFVNAPADFVNSVLAAVPLDVLQFHGDESEEFCRSFARPYVKALRMKPGI
                     DLAAAAKTYDSAQGLLVDAWHEEQFGGTGIAFDWALLGAAIRNERLVLAGGLHPGNVA
                     QAIRQVRPWAVDVSSGVESAPGIKDKGKIEQFISEVQRA"
     gene            595..>1342
                     /locus_tag="VEA63_13880"
     CDS             595..>1342
                     /locus_tag="VEA63_13880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007358624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HYD85148.1"
                     /translation="MPDKQNASSNPSSFKSYDSPDARGHFGPYGGVFVGETLMPALEQ
                     LNRKYAELKQDPAFWAEFDYDLAHYVGRPSPLYFAQRMTRELGGAQIFLKREDLNHTG
                     AHKVNNTIGQALLAKAMGKTRIIAETGAGQHGVATATVAARLGLECHVYMGIDDIARQ
                     TANVFRMKILGANVVGVTSGSRTLKDAMNEALRDWATNVDNTYYIIGTVAGPHPYPML
                     VRDFQSIIGREVKEQSMKQNGRLPDSLIACV"
BASE COUNT          269 a          414 c          430 g          229 t
ORIGIN      
        1 tatgcggcat tacgcgtgtc gaagacggcg tcgcggccgc ggcggcaggt gcggatgcga
       61 tcggcctgaa cttctgcgag aagagtccgc gcgtgatcga tgcggaacgc gcgcggagtt
      121 tttgcgcggc gctaccgcca ttcgtcgcgc gcgtcggcct gttcgtgaat gcgcctgcgg
      181 acttcgtcaa cagcgtgctc gccgccgtgc cgctcgatgt actgcagttc cacggcgatg
      241 aaagcgaaga gttctgccgc agtttcgccc ggccttacgt gaaggcattg cggatgaagc
      301 cgggcatcga tctcgccgcc gcagcgaaaa cctatgactc cgcgcaaggc ctgctcgtcg
      361 atgcatggca cgaagagcag ttcggcggca ccggcatcgc attcgactgg gccttgctcg
      421 gcgcggcgat acgcaacgaa cgcctcgtgc tcgccggtgg tctgcatccg ggcaacgttg
      481 cacaggcgat caggcaggtg aggccatggg ccgtcgacgt cagttccggc gtcgagagcg
      541 cgcctggcat caaggacaaa ggcaagatcg aacaattcat cagtgaggtg cagcgtgcct
      601 gataaacaga acgcatccag caatccatcg agcttcaaga gctatgacag tcccgacgcg
      661 cgcggccatt tcggtccgta cggcggcgtc ttcgtcggcg aaacgctgat gccggcgtta
      721 gagcagctca atcgcaaata tgcggagctg aagcaggatc cagccttctg ggccgaattc
      781 gattacgacc tggcgcatta cgtcggtcgt ccgtcgccgc tgtatttcgc gcagcgcatg
      841 acgcgcgaac tcggcggcgc gcaaatcttc ctgaaacgcg aagacctgaa ccacaccggc
      901 gcgcacaagg tgaacaacac gatcggccag gcgctgctcg cgaaagcgat gggcaagacg
      961 cgcatcatcg ccgaaaccgg cgcgggccag cacggtgtgg cgacggcaac ggttgccgcg
     1021 cgcctcggtc tcgaatgcca cgtgtacatg ggcatcgacg acattgcgcg ccagaccgcg
     1081 aacgtgttcc gcatgaagat cctcggcgcg aacgtcgtcg gcgtcacgtc gggttcacgc
     1141 acgctgaaag acgcgatgaa cgaagcgctg cgtgactggg cgacgaacgt cgacaacacg
     1201 tactacatca tcggcaccgt tgccggtccg catccgtacc cgatgctcgt gcgcgatttc
     1261 cagagcatca tcggccgcga agtgaaagag cagagcatga agcagaacgg tcgtttgccc
     1321 gattcgctga tcgcctgcgt cg
//
LOCUS       DASPLT010001365         2322 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_159163, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001365 DASPLT010000000
VERSION     DASPLT010001365.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 2322)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2322)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2322
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_159163"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..299)
                     /locus_tag="VEA63_13885"
     CDS             complement(<1..299)
                     /locus_tag="VEA63_13885"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009513411.1"
                     /note="involved in lysine biosynthesis; DAP epimerase;
                     produces DL-diaminopimelate from LL-diaminopimelate;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HYD85149.1"
                     /translation="MRFHKYHALGNDYIVCDPRDFPSWNPAPTVDQIRVVCHRNFGVG
                     SDGILWGPLPSKQSEFGLRIFNPDGSEAEKSGNGLRIFSRYLYDQKLVKNPSFTI"
     gene            complement(417..1097)
                     /locus_tag="VEA63_13890"
     CDS             complement(417..1097)
                     /locus_tag="VEA63_13890"
                     /inference="COORDINATES: protein motif:HMM:NF025594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4336 domain-containing protein"
                     /protein_id="HYD85150.1"
                     /translation="MLTALHPDLHIHRVPYRALGLPIGRQLVVVRLPSNELWIHSPIP
                     WSPALREAVWHLGEVGHVVGPNRFHDECLREFQAEYPRADFHAAPGLAHARRDIRFQT
                     EPLGNTPPAAWAGVLDQHLVAGMPRLNEIVFLHRPSRTLIIADLAFNLGPDGPWLLAL
                     IMKLNHAWGRFTPTRFFKSMIKDRAAVRASIEHILAWDFDRILVGHGANIETNGKAAL
                     REAFAFLK"
     gene            complement(1292..>2322)
                     /locus_tag="VEA63_13895"
     CDS             complement(1292..>2322)
                     /locus_tag="VEA63_13895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009514197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HYD85151.1"
                     /translation="ALGIVGTNLIYGAFYFRDDPKKFTESLLDNLSADRIEVDMLKFS
                     GPAFKHVDNRLMSLYLVQFGLTNAVMFGPKGDVLQPSEVLHKKAILVERGSFRPVTHV
                     NVDMLNCATAQFVQEPLVKGKEFVVLMEITMNNLLAAGSLDAQDFLSRVDLLGDIGFT
                     VLISNYSEYFRLTSYFRRYTKEMIGVAMGINNLLEIFNEKYYENLEGGILESFGRLFR
                     HSVKLYIYPMRQDAYGHYLGTGEVTPAPTATSANSFASSVLIHAKNVQVVDHLRNLYN
                     HLLENHYIECIAGFDPNILHIFSRDVLRRIKESDPSWEEMAPPAVVAAIKNRGLFGYA
                     PPTAAAAS"
BASE COUNT          469 a          563 c          856 g          434 t
ORIGIN      
        1 tcgatggtga aggacggatt tttgacgagc ttctgatcgt agagataacg ggaaaaaatg
       61 cgcaggccgt tgccggattt ttccgcttcg gagccgtcgg gattgaaaat gcgcaggccg
      121 aactcgctct gcttggacgg cagagggccc cagagaatgc cgtcggagcc gacgccgaag
      181 ttgcgatggc agacgacgcg gatttgatcg acggtcggcg cgggattcca agaggggaaa
      241 tcgcgcggat cgcagacgat gtagtcattg ccgagagcgt ggtatttgtg gaagcgcatg
      301 gagcggcgaa aaggtagcga aaaggtagcg aaaaagtagt gagtagcggg tagcgggtag
      361 cgagtagaaa acgcggaggt gcaggcaggc gttcactgcc cgctactcgc tcctcgctac
      421 ttcaaaaacg cgaaggcttc gcggagggcg gctttgccgt tggtttcgat gttggcgccg
      481 tggccgacga ggatacgatc gaagtcccag gcgagaatgt gctcgatcga cgcgcgcacg
      541 gcggcgcggt ctttgatcat gcttttgaag aaacgtgtcg gcgtgaagcg gccccacgcg
      601 tggttgagtt tcatgatgag cgcgagcaac cacggcccgt cggggccgag attgaacgcg
      661 agatcggcga tgatgagggt gcggctcggc cgatggagaa aaacgatttc gttcagccgc
      721 ggcatgcctg cgacgaggtg ttggtcgagc acgccggccc atgcggcggg cggcgtgttg
      781 cccagcggtt cggtttgaaa gcggatgtcg cgacgcgcgt gggcgaggcc gggtgcggcg
      841 tggaagtcgg cgcggggata ctcggcttga aactcgcgaa ggcattcgtc gtggaagcgg
      901 tttggaccaa cgacgtggcc gacttcgccg aggtgccaca cggcttcgcg caacgcgggc
      961 gaccacggaa tcggcgagtg aatccacagt tcgttggacg gcaggcgcac gacgacgagt
     1021 tggcggccaa tgggaagacc gagcgcgcgg taaggcacac ggtggatgtg caggtcggga
     1081 tggagcgcgg tgagcatgaa tggcgggcgt tctcgttctc gttctcgttc tcgttctcgt
     1141 tctccgcggc ggcggctagc cgaaaattgg acgggagaga acgattaaga gaaagagaac
     1201 gagaacgaag ccggagggca gggccggcgc gacggacgcc tcggagaggc gtccctaccg
     1261 actcgggcgc gagagggttg acgtttgctc gctagctcgc ggccgcggcg gtgggcggag
     1321 cgtagccgaa gaggccgcgg tttttgatcg cggcgacgac ggcgggcggc gccatttctt
     1381 cccacgaggg atcgctttcc ttgatacgac gaaggacgtc gcgggaaaaa atgtgcagga
     1441 tgttcggatc gaaaccggcg atgcactcga tgtagtgatt ctctagaagg tgattgtaga
     1501 ggttgcgcaa gtgatcgacg acctgaacgt ttttcgcgtg gatgaggacg gaggacgcga
     1561 aagagttggc gctcgtggcg gtgggggcgg gcgtcacttc gccggtgccg agatagtgac
     1621 cgtaggcgtc ctggcgcatc gggtagatgt agagcttcac cgaatggcgg aagagccggc
     1681 cgaaggattc gaggatgccg ccttcgaggt tctcgtagta tttctcgttg aagatctcga
     1741 ggaggttgtt gatgcccatc gcgacgccga tcatctcctt cgtgtatcga cgaaagtagg
     1801 acgtgagtcg gaagtattcg gagtagttgg aaatgaggac ggtgaagccg atgtcgccga
     1861 ggagatcgac gcgcgaaagg aagtcctgcg cgtcgagcga gccggcggcg aggaggttgt
     1921 tcatcgtgat ctccatcaac acgacgaact cctttccctt cacgagcggc tcctggacga
     1981 actgcgcggt ggcgcagttg agcatgtcga cgttgacgtg ggtgacggga cggaagctgc
     2041 cgcgttcgac gaggatggct tttttgtgaa gcacctcgga cggctggagc acgtcgccct
     2101 tgggaccgaa catcacggcg ttggtgaggc cgaactggac gaggtagagc gacatcagac
     2161 ggttgtccac gtgcttgaac gcggggccgc tgaacttgag catgtcgacc tcgatgcgat
     2221 cggcgctgag gttatcgaga agggactcgg tgaatttctt cgggtcgtcg cggaagtaga
     2281 acgcgccgta gatgagattg gtgccgacaa tcccgagggc gt
//
LOCUS       DASPLT010001366         1906 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_201370, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001366 DASPLT010000000
VERSION     DASPLT010001366.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1906)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1906)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1906
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_201370"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..256)
                     /locus_tag="VEA63_13900"
     CDS             complement(<1..256)
                     /locus_tag="VEA63_13900"
                     /inference="COORDINATES: protein motif:HMM:NF019614.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1697 domain-containing protein"
                     /protein_id="HYD85152.1"
                     /translation="MPHYVAFLAGINLGNRRLKMDALRAHFEALKFRNVATFIASGNV
                     IFDSSARDAAKLETQIERHLAKTLGYEVATFLRTREEVAAI"
     gene            438..677
                     /locus_tag="VEA63_13905"
     CDS             438..677
                     /locus_tag="VEA63_13905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD85153.1"
                     /translation="MATPTEQNFEDYKRAEKKALEIVTAMKAVTPKKVDIELALLVAI
                     FELHKGTLPPDTIGAIVQGHLKQLLPYYAGKTASG"
     gene            complement(724..1290)
                     /locus_tag="VEA63_13910"
     CDS             complement(724..1290)
                     /locus_tag="VEA63_13910"
                     /inference="COORDINATES: protein motif:HMM:NF013052.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SET domain-containing protein-lysine
                     N-methyltransferase"
                     /protein_id="HYD85154.1"
                     /translation="MPRLTKFNITSSSKVPRVSSPWIRVSNSAIHGRGVYARVAIPDG
                     TRIIEYVGERITKAEALRREEQRLARLRAGGDGSVYIFILNKRHDLDGRHSNNVTRLI
                     NHSCSPNCRAETIRGHIWIIANRDIAPGEELSFDYGFPFGEWRKHPCRCGAPGCVGYI
                     VNKAQRWRVARILREERRAAKAARTAAG"
     gene            complement(1395..>1906)
                     /locus_tag="VEA63_13915"
     CDS             complement(1395..>1906)
                     /locus_tag="VEA63_13915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009509562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HYD85155.1"
                     /translation="IISRSDHIAIDTFYVVEPGRGVVQNTSAQETFARTIEAALVSNK
                     DLYPDIVAQAKKHAASRFTTGNNGEALHTSFPPTVEVYHELAMQRTIVEIQARDQIGL
                     LYRLAKIISDQGFDITFARIGTERGVAIDTFYIESTEPTQPIDTPRLQALRDTLMEVI
                     KPATVAAAS"
BASE COUNT          344 a          489 c          697 g          376 t
ORIGIN      
        1 cgatcgccgc gacttcctcg cgcgtgcgaa gaaaggtggc gacttcgtag ccgagcgttt
       61 tggcgaggtg gcgttcgatc tgggtttcga gctttgcggc gtcgcgcgcg ctcgagtcaa
      121 agattacgtt tccgctggcg atgaaagtgg cgacgttgcg gaacttcagg gcttcgaaat
      181 gggcacgaag cgcgtccatt tttaggcggc ggttgccgag attgattccg gcgaggaagg
      241 cgacgtaatg gggcatggtg gatgcggcag gcagaactaa accaggcggc gttggtgttt
      301 tcggaaacga ttttcctgcc gacaaggacg tctgcaaaca aatgcggcgt ggccgcgagc
      361 ggagcgttgg ggcgcgctgg tggagcagag gacgtttggg ccttgacgaa tcggtttggg
      421 agcgaggtcg ttgagggatg gcgacgccga cggaacagaa tttcgaagat tacaaacgcg
      481 cggagaaaaa ggcgctcgag atcgtgacgg cgatgaaggc cgtcacgccg aagaaagtgg
      541 atatcgagct ggcgttgctc gtggcgattt tcgagctgca caaaggcacg cttccgcccg
      601 acacgatcgg cgcgatcgtg cagggtcacc tcaagcaatt gctcccgtat tacgccggga
      661 aaacggcgtc gggctaacga ccccggccgg ccgttgcgta ttggcgttcg gcgaaaaggc
      721 gggtcaaccg gcggcggtgc gcgcggcttt ggcggcgcgg cgctcttcgc gcagaatgcg
      781 cgcgacgcgc cagcgttggg ctttgttgac gatgtagccc acgcaacccg gcgcgccgca
      841 gcggcacgga tgtttccgcc attcgccaaa ggggaaaccg tagtcgaagg agagctcctc
      901 gccgggcgcg atgtcgcgat tggcgatgat ccagatgtga ccgcgaatcg tctcggcgcg
      961 gcaattcggc gagcacgagt gattgatcag gcgggtgacg ttgttcgagt ggcgtccatc
     1021 gagatcgtgc cgcttgttga gaatgaaaat gtagacgctg ccatcgccgc cggcgcgaag
     1081 tcgtgcgagg cgttgttctt cacggcggag cgcttcggct ttggtgatgc gttcgccgac
     1141 gtattcgata atgcgcgtgc cgtcggggat cgcgacgcgg gcgtaaacgc cgcggccatg
     1201 aatggcggag ttgctcacgc gaatccaggg actggagaca cgcggcacct tcgaggagga
     1261 ggtgatgttg aacttggtca aacgaggcac gcggcactcg gtaagcgagt gcggggtttt
     1321 ggcaactgtg gtcacgcgga cgatgcgaag gcgcgagccg cggaggcggc ctcggctgtg
     1381 agggaacggt gaccttagct cgctgccgct acggtggccg gcttgatcac ttccatcagc
     1441 gtgtcgcgca gcgcctgcaa acgcggggta tcgatcggct gcgtgggctc ggtgctttcg
     1501 atatagaacg tgtcgatcgc gacgccgcgt tcggtgccga tgcgcgcaaa ggtgatgtcg
     1561 aagccctggt cggagatgat ttttgcgagg cggtagagga ggccgatctg gtcgcgggcc
     1621 tggatctcca cgatcgtgcg ttgcatcgcg agctcgtgat aaacttcgac ggtgggcgga
     1681 aaggacgtgt gcagcgcctc gccattgttc ccggtggtaa aacggctggc ggcgtgtttt
     1741 ttcgcctgcg cgacgatgtc gggatagagg tctttgttgg agacgagcgc ggcctcgatt
     1801 gtccgggcga acgtttcctg cgcgctggtg ttttgaacga cgccgcggcc gggttcgacg
     1861 acgtagaacg tgtcgatcgc gatgtgatcg gagcgcgaga tgattt
//
LOCUS       DASPLT010001367         1223 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_201373, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001367 DASPLT010000000
VERSION     DASPLT010001367.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1223)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1223)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1223
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_201373"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1223)
                     /locus_tag="VEA63_13920"
     CDS             complement(<1..>1223)
                     /locus_tag="VEA63_13920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009508953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD85156.1"
                     /translation="NSPVYNIDDYVHAVGRDVIAFVEMNNELDMTSQQANTYWHNLSE
                     PLSNDPVSPYYYADYAQAATADTWSELISHSDPSIASLPLIGPSLVSDAAYTTVGDLS
                     DSIEYSNIHHYLYGWNPEVTNIRGIDHAIANQSLVQGPNRGMTTTEGGWASATALTGS
                     LPVVTHGRYLPRYFLAHFLKGFAITTSYEFVDEGTNPANQEDNFGLLKNDLTPKPAYT
                     ALKNLLSVLSDPGPSFAPGSLDYSMSGSTADVASALFRKRNGDYYLCLWLALPSYHPV
                     NGTAIAVPAQSISLALPASIVSAQIFSLDDAGAMTASAGTISSHTLNLSVTDRVTVVR
                     LSTISTGGVASTPHGLRAAPASGQIVLKWAPVFEAATYSVKRAASLNGPYSTIASGLT
                     SPTFTDTGRTNGTPC"
BASE COUNT          247 a          303 c          439 g          234 t
ORIGIN      
        1 agcaaggcgt gccgttggtc cggcctgtat ccgtgaaagt aggactggtg aggcccgagg
       61 cgatggtgct gtaggggccg ttgaggctgg cggcgcgttt gacgctataa gttgcggctt
      121 cgaaaacggg cgcccatttc aaaacgattt gtccgctggc aggcgcagcg cgcaggccat
      181 gcggcgtgga agcgacgccg ccggtggaaa tggtgctgag acgaacgacg gtgacgcgat
      241 cggtgacgct caggttgagc gtgtggctgc tgatcgtgcc ggcagaggcg gtcatcgcgc
      301 cggcgtcgtc gagcgagaag atctgggcgc tgacgattga cgcaggcaac gcgagggaga
      361 tcgattgcgc ggggacggcg atcgccgttc cgttaaccgg atgataggaa ggcaacgcga
      421 gccagagaca aaggtagtaa tcgccgttgc gtttgcggaa gagggcggag gcgacatccg
      481 cggtgctgcc cgacatgctg taatccagag agccgggagc gaacgacgga ccgggatcgc
      541 tcagcacgga gagcaggttc ttcaacgcgg tgtaggcggg cttgggagtg agatcgtttt
      601 tgagcaggcc aaagttgtcc tcctgattcg cgggattcgt gccctcgtcg acgaactcgt
      661 aggaggtcgt gatcgcgaaa cctttgagaa aatgcgcgag gaaataacgc ggaagataac
      721 ggccgtgcgt gacgaccggg agcgaacccg tcagcgcggt ggccgaggcc cagccgcctt
      781 cggtcgtcgt catgccgcgg ttgggtccct ggacgagcga ctggttcgca atcgcatgat
      841 cgatgccgcg gatgttggtg acctcagggt tccatccata aagataatga tgaatgttac
      901 tgtattcgat gctgtcgctg aggtcaccga ccgtcgtgta ggcggcgtcg gagacgagag
      961 agggaccgat gagcggcaag ctggcgatgg aaggatcgct gtggctgatc aactcgctcc
     1021 acgtgtccgc cgtcgcggcc tgtgcatagt cggcgtaata gtaaggcgaa acgggatcgt
     1081 tcgagagcgg ctcgctaaga ttgtgccaat acgtgttggc ctgctgcgaa gtcatatcga
     1141 gttcgttgtt catttcgacg aacgcgatga cgtcgcgccc gacggcgtgc acataatcat
     1201 cgatgttgta gacgggagag ttc
//
LOCUS       DASPLT010001368         1327 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_140089, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001368 DASPLT010000000
VERSION     DASPLT010001368.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1327)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1327)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1327
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_140089"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..377)
                     /locus_tag="VEA63_13925"
     CDS             complement(<1..377)
                     /locus_tag="VEA63_13925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007361228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HYD85157.1"
                     /translation="MSSSEQKNLLTTNRKALTINLDDQKYGTFAEIGAGQEVARIFFQ
                     AGGASGTVAKTISAYDMAFSDAIYGKAPRYVSRERLNLMLDHEYQLLIERLASSRGDR
                     TTFFVFADTVAARNYKGTNEAHG"
     gene            509..>1327
                     /locus_tag="VEA63_13930"
     CDS             509..>1327
                     /locus_tag="VEA63_13930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HYD85158.1"
                     /translation="MKNFFTSMLGALVALIIFTVGGALLFIGFIGALAAIGGEKPVSV
                     EEGSYLVLDLAAEVTDAPPVIDLRLFGADRAPTLQLRTITRTLHAAANDERIAGVFIK
                     GDAPPAGFGPGYATLKEIRGALEAFKASGKPVKAYLTYATTKNYYLASAADELVLDPY
                     GLIFMPGLSSQPVFVAGAFEKYGVGVQVTRVGKYKSFVEPFTRKDMSPESREQMQKLL
                     DDLWGGLLADVSATREVSVDAIQRTVDAEGVIRAEAAVEAKLIDRVAYRDEVIDE"
BASE COUNT          250 a          353 c          470 g          254 t
ORIGIN      
        1 cagccatggg cctcgttggt gcccttgtaa ttccgcgcgg cgacggtgtc ggcgaagacg
       61 aaaaacgtgg tgcgatcgcc gcgggaggag gcgagccgct cgatcaggag ttgatactcg
      121 tgatcaagca tgaggttgag ccgctcgcgc gaaacatagc gcggggcctt gccgtagatc
      181 gcgtcgctga acgccatatc gtaggcagag atcgttttcg cgaccgtccc ggacgcgccg
      241 ccggcctgga agaaaatgcg agcgacttcc tggccggcac cgatctcggc aaacgtgccg
      301 tatttctgat cgtccaaatt gatcgtcagc gccttgcggt tggtggtgag gaggttcttt
      361 tgctcgctcg aagacatggc gcggaactat gcggctgcca tgaagcggcg caatccgatc
      421 gtggccgcgg gcggtgggcc ttctgggaaa gagctgggat tgaactggag gaacgaacga
      481 aagcgcgtag ggtcgccgct tcctgcgtat gaaaaatttc ttcacgtcga tgctcggtgc
      541 gctggtggcg ttgatcatct tcacggtggg cggagcgctg ttgttcatcg ggttcatcgg
      601 agcgctggca gcgatcgggg gtgagaagcc ggtgagcgtc gaagaaggct cctacctcgt
      661 gctcgacctc gcagccgaag tgacggatgc gcccccggtg atcgacctgc ggttgttcgg
      721 cgccgatcgt gcgccgacgt tacagcttcg gaccatcacg cgaacgctgc acgcggcggc
      781 gaacgacgag cggatcgccg gcgtgttcat caagggcgat gccccgccgg cggggttcgg
      841 gccaggctat gcgactttga aggagattcg cggagcgctc gaagcgttca aggcgtcggg
      901 caagcccgtg aaagcttatc tcacctacgc aacgacgaag aactactatc tcgcgtccgc
      961 ggcggacgaa ctcgtgctcg atccgtatgg attgatcttc atgcccggcc tttcgagcca
     1021 gccggtgttc gtggccggcg ctttcgaaaa atacggtgtc ggcgtgcagg tgacgcgggt
     1081 cgggaaatac aaatcgttcg tcgaaccgtt cacgcggaaa gacatgagtc cggagagccg
     1141 cgagcagatg cagaagctgc tcgatgattt atggggcgga ctgctcgcgg atgtgagtgc
     1201 gacgcgggag gtgagcgtgg atgcgataca gcggaccgtc gatgcggagg gcgtgatccg
     1261 cgcggaggcg gcggtggaag cgaagctgat cgatcgggtc gcttatcgtg acgaggtgat
     1321 cgatgag
//
LOCUS       DASPLT010001369         1054 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_55788, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001369 DASPLT010000000
VERSION     DASPLT010001369.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1054)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1054)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1054
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_55788"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            549..713
                     /locus_tag="VEA63_13935"
     CDS             549..713
                     /locus_tag="VEA63_13935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD85159.1"
                     /translation="MSHEQDSKAAKIRSILRETFGDKIDAEIVDKVANRALRAPPVFH
                     PRKTFPGAKR"
     gene            869..>1054
                     /locus_tag="VEA63_13940"
     CDS             869..>1054
                     /locus_tag="VEA63_13940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD85160.1"
                     /translation="MNKLYPSPFIAEVAYDDTDALIIGQYHCCVFEDAAASFSRRLPR
                     RWTGCGMITPFLRHLTDA"
BASE COUNT          221 a          334 c          305 g          194 t
ORIGIN      
        1 cgatcccaga acgcggtgcg gcaaacctag attaaatatt ggccgagcga gatctcgcga
       61 cggccgggat tgacctcgac ttaacgcagg cagtagagcc gcctgcatgg atcgtgcaat
      121 ccgcgtggcg gaaccctcca ccggtctcgt tgggcaatct ggaggattgg agaaacgctc
      181 catgcgtatc gagcgacatc gcacgtcggt ggcgcttgaa cccgcgtttt gggctgcgct
      241 ggaaagcatt gcccgcgccc atcgaaagac catcccgacg ctcctcgccg aaatcgatcg
      301 gcggcgggcg gcttcttacc cagatcgcag cctcgcatca gcagcacggg tatttgccct
      361 gcagcaccgt ccgtaggcgc cgcttttgcg aggcctagcc gaccagcgcc gcaatacgaa
      421 agactaaggg caataaaaat tagcgagcgc ctcatgtcat cgcgcgggcg aagctccggc
      481 tacacaaatt cgccttctac aacttcagcg tcattgcgca cgcctcggcg ccaaataacg
      541 ctttgcgcat gtcgcatgag caggattcca aggccgcgaa gatacgctcg atattgcgcg
      601 agacgttcgg cgataaaatc gacgctgaaa tcgttgataa agtcgccaac cgagctctgc
      661 gcgcgccgcc ggtattccac ccgcgcaaga cctttcctgg cgccaagcgc tgaacccggc
      721 tctgcgaggc cgactcgcag aagcgaccgg ttcaatcgga tcggaagcga acaccggcgg
      781 ccgcgtcctg ctgccagacg agctccaccc tccgccgcaa tttcatcgtg ggctcgcata
      841 agatgaagga gctcggcaac agccggcgtt gaacaaactc tatccgagcc ccttcatcgc
      901 tgaggtcgcg tacgacgaca ctgacgcgct catcattggg cagtatcact gttgcgtttt
      961 tgaagacgcc gcggcgagtt tttcgcgccg cctgccgcgg cgctggaccg ggtgtgggat
     1021 gataacaccg ttcttgcgtc acttgacgga cgcg
//
LOCUS       DASPLT010001370         1081 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_201482, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001370 DASPLT010000000
VERSION     DASPLT010001370.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1081)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1081)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1081
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_201482"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1081)
                     /locus_tag="VEA63_13945"
     CDS             complement(<1..>1081)
                     /locus_tag="VEA63_13945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007358797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF255 domain-containing protein"
                     /protein_id="HYD85161.1"
                     /translation="ELKPFFGGTYFPPEDRGGRAGFASILRAIAKGWAEERDKLIAEG
                     DRAIEALRGHYEEKAEGEASGGASGEQSLVESGATAFEKCFQYFYEAFDPDHGGFGSA
                     PKFPRASNLTFLFRAAALQGVASEAGAEAVKLAAATLQAMARGGIHDHVGGGFHRYSV
                     DATWFVPHFEKMLYDQAQIAVNALEAKQATGDERFAWMARDVFEYVLRDLSHPEGGFY
                     SAEDADSLPSEAVGAEGSDKGQVTSDKKESGAHAVEGAFYVWTRAEIAAALGDDAPMF
                     AAHFGIAENGNVPEDLDPHGELRGKNILAQRQSLAATARAYGLELEAASERIAAALAK
                     LRAVRERRPRPLRDDKIITAWNGLMI"
BASE COUNT          182 a          441 c          260 g          198 t
ORIGIN      
        1 aaatcatcaa cccgttccac gccgtgatga tcttgtcgtc gcgcaacggc cgcggtcgcc
       61 gctcgcgcac cgcccgcagc tttgccagcg ccgccgcgat tcgctcgctc gctgcctcga
      121 gttccagccc gtaagcgcgc gccgtcgccg ccagcgactg ccgctgcgcc agaatatttt
      181 tcccgcgcaa ttcaccgtgt ggatcgaggt cctccggcac gttcccgttc tccgcgattc
      241 caaaatgcgc cgcaaacatc ggcgcatcgt cgcccagcgc cgccgcgatc tccgcgcgcg
      301 tccaaacata aaacgccccc tccaccgcgt gcgccccact ctccttcttg tcacttgtca
      361 cttgcccctt gtcacttccc tccgctccca ccgcctccga cggcagcgag tccgcatctt
      421 ccgccgaata aaacccgcct tccggatggc tcagatcccg taacacgtat tcgaatacat
      481 cgcgcgccat ccacgcgaag cgctcgtcgc ccgtcgcctg cttggcctcg agcgcgttca
      541 ccgcgatctg cgcctgatcg tagagcatct tctcgaaatg cggcacgaac cacgtcgcat
      601 cgaccgagta gcggtgaaac ccgccgccga catgatcgtg aatccccccg cgcgccattg
      661 cctgcagcgt cgccgccgcc agcttcaccg cctccgcgcc cgcctcgctt gcgacaccct
      721 gcagcgccgc cgcgcgaaac aaaaacgtca gattgctcgc gcgcggaaat ttcggcgcgc
      781 tcccgaatcc gccgtgatcc ggatcgaacg cctcgtagaa atattgaaag cacttctcga
      841 acgccgtcgc accgctctcg accagcgact gctccccgct cgctcccccc gacgcctcac
      901 cttcggcttt ttcctcatag tgcccgcgca acgcctcgat cgcgcgatcg ccttccgcga
      961 tgagtttatc ccgctcctct gcccagcctt tcgcgatcgc gcgcaaaatc gacgcgaacc
     1021 ccgcccgccc gccgcgatcc tccggcggaa aatacgtgcc accgaaaaac ggtttcagtt
     1081 c
//
LOCUS       DASPLT010001371         2192 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_55828, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001371 DASPLT010000000
VERSION     DASPLT010001371.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 2192)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2192)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2192
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_55828"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..246
                     /locus_tag="VEA63_13950"
     CDS             <1..246
                     /locus_tag="VEA63_13950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007359986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HYD85162.1"
                     /translation="EVNDLHRREFLRGYLRETLRAIGDGVPVRGYFLWSFMDNFEWED
                     GYARRFGIVHVDFATQRRTPKLSAHYYSTVIRANRIV"
     gene            425..>2192
                     /locus_tag="VEA63_13955"
     CDS             425..>2192
                     /locus_tag="VEA63_13955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD85163.1"
                     /translation="MFSTTRPGVARPSLAGASAIVFATLLVLVSGSVRAHTPDPVAQQ
                     PVGSRVLTSFDEIWQLPQAEHQQWHRLKFDYVVYYYDPLWQAMWGRSREAESYLSLGA
                     KVFPIKAGQRIQVEGLMRPAGGMRVEDAEVTVLEESEPLPAISTKGAVSSVERFNKRR
                     VTVEGYVDRQAARDANHLEVALIAEGRPVLVQLLLRNDTPVPQLRDKFIRATGVYFAR
                     SDDGLDGAKIELWVQRKEDIEVFGTLDRDPRFERPATPANALAEQAGDGLVRVTGVVV
                     AQQPGHSLTIRQSETELRLNTAQTLNLRAGEEVEAVGFPVRDASGWALRESLVRTLRP
                     VFTSVNQIWKLRDEDRGRWQRVNLDVVVYYFDPLWRGLWGNIGGTEDYISLGEQDFGL
                     KPGQRIRVEGSVLVENGVLVDSPKVTVLDEAVPLEALSTAGRIRDAEKFNKRLVTVEG
                     YVDRQGFSDARHGLLDLVVDGRPVIGRLLVAEGETPPNWEGKFVRLRGVYSATQDPAG
                     GTTSIEIWTQGTENVDVLGTLDLDERFSLPLTRIDQLASLPAGTAVHVRGIVRAQQPG
                     RLLTIRDETGQMDLVTAQARPTE"
BASE COUNT          375 a          541 c          869 g          407 t
ORIGIN      
        1 gaggtcaacg atttgcaccg gcgcgagttt ttgcgcggct atttgcgcga gacgttgcgg
       61 gcgatcgggg acggcgtgcc ggtgcgcggc tactttttgt ggtccttcat ggataatttc
      121 gaatgggagg acggttatgc gcggcggttc gggatcgtgc atgtcgattt cgcgacgcag
      181 cggcggacgc cgaaactttc ggcgcattat tattcgacgg tgatccgcgc gaaccggatc
      241 gtgtgaaccg acttgcagcg ctcgcgcgag cgggcggcga cgcggcggcg caaggacggc
      301 gccagacgcg ggagaaatcg caaacgatga gcggcggacg cgccgcggat tcggtttaat
      361 cggcaccgtt cccgtgacga agggagggga tttaacggtg cagttttttg agggcggtat
      421 tttcatgttt tcaaccacac gaccgggtgt tgcgcggccg tcgctggccg gcgcgagcgc
      481 gatcgttttc gcgacccttc tcgtgttggt gagcgggagt gtgcgtgcgc acacgcccga
      541 tcccgtcgcg cagcagccgg ttgggtcgcg cgtgctgacg agtttcgacg agatctggca
      601 gctcccgcaa gcggagcacc agcagtggca ccggctgaaa ttcgattacg tcgtctatta
      661 ctacgatccg ctgtggcagg cgatgtgggg acgttcgcgc gaggccgaga gttatctgtc
      721 gctgggagcg aaggtgtttc caatcaaagc gggccagcgg attcaggtgg aaggcttgat
      781 gcggccggcg ggcggcatgc gcgtggagga cgcggaggtg acggtgctgg aggaatcgga
      841 gccgttgccg gcgatttcga cgaaaggggc ggtgagtagc gtcgagcgtt tcaacaagcg
      901 gcgcgtgacg gtggagggtt atgtcgatcg tcaggcggcg cgcgacgcga accatctcga
      961 ggttgcgctg atcgcggaag gccggccggt tttggtgcag ctgttgttga ggaacgacac
     1021 gccggtgccg cagttgcgcg acaagttcat ccgggcgacg ggagtgtatt tcgcgcggag
     1081 cgacgacggg ctcgacggcg cgaagatcga gttatgggtg cagcggaaag aggacatcga
     1141 agtgtttggc acgctcgatc gcgatccgcg tttcgagcgg ccggcgacgc cggcgaacgc
     1201 gctggcggag caagcgggcg acgggctggt gcgggtcacc ggcgtggtgg tggcgcaaca
     1261 gccggggcat tcgctgacga ttcgccaaag tgagacggag ctgcggttga acacggcaca
     1321 gaccttgaac ctgcgggcgg gtgaagaggt ggaggcggtg ggctttccgg tgcgcgacgc
     1381 gagcggctgg gcgctgcggg agagtctcgt gcgcacgctg cgtccggtgt tcacgagcgt
     1441 gaaccagatt tggaaactgc gggatgagga tcgggggaga tggcagcgcg tgaacctgga
     1501 tgtggtcgtg tattatttcg atccgctgtg gcgtgggttg tgggggaaca tcggcgggac
     1561 ggaggattat atctcgctcg gtgaacagga ttttggactg aagccgggcc agcgcattcg
     1621 ggtcgagggc tcggtgttgg tggagaatgg cgtgctggtg gacagcccca aggtgacggt
     1681 gttggacgaa gcggtgccgc tggaggcgtt gtcgacggcg gggcggattc gcgatgcgga
     1741 aaagttcaac aagcggctgg tgacggtcga aggctatgtc gaccgtcagg ggtttagcga
     1801 cgcgcggcat gggctgctgg atttggtggt ggacggccgg ccggtgatcg ggcggctgct
     1861 ggtggcggaa ggcgagacac cgccaaattg ggagggcaag ttcgtgcggt tgcgcggcgt
     1921 gtattctgcg acgcaagatc cggccggcgg cacgacgagc atcgagattt ggacgcaggg
     1981 cacggagaat gtggacgtgc tcggaacgct ggatctcgac gagcgattca gcctgccgtt
     2041 gacacggatc gatcaactgg cgtcgctgcc ggcgggcact gcggtgcacg tgcggggcat
     2101 cgtgcgcgcg cagcagccgg ggcggttgtt gacgattcgc gatgagacgg gacagatgga
     2161 tctggtcacc gcgcaggcga ggccgaccga ga
//
LOCUS       DASPLT010001372         1085 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_303707, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001372 DASPLT010000000
VERSION     DASPLT010001372.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1085)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1085)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1085
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_303707"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1085)
                     /locus_tag="VEA63_13960"
     CDS             complement(<1..>1085)
                     /locus_tag="VEA63_13960"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HYD85164.1"
                     /translation="TERPVNATPGTINVIDLTEPDGVTLDQFVRGETLVSVPFAFSGA
                     GVAYQRGGAQSINIRGVEGNRVLLQVDGVRVPDEFALGGSEPTGRDYFDPELFKRVEI
                     LQGSASALYGSDALGGVVTFTTKSPADYLNGKRIYAGAKAAYRSVDDGTSLSATFAAA
                     HGPLQALVVYSHHEGHERENNGIVRPNPEDYSSDAVLTKLVWLPSAPHRFELTLENFD
                     RDLTAQVNNKEIMSGATTTTGLDLDSTTQRFRLGAAYLFDGNAPLFDQLEVRAYLQDA
                     NTHDVAYERFAYNPPSAANGAFRHRDITTEFHNDTTGFSVAAVKSLGAAHRLAYGIEG
                     SRTETSKPWHSVVTNSFGTTRPTEPRM"
BASE COUNT          168 a          261 c          451 g          205 t
ORIGIN      
        1 catacgcggc tcggtggggc gcgtcgtgcc aaagctgttg gtgacgacgc tgtgccacgg
       61 tttgctggtt tcggtgcgcg agccttcgat gccgtaggcg aggcggtggg cggcgccgag
      121 ggattttacg gcggcgacgg agaagccggt ggtgtcgttg tgaaattcag tcgtgatgtc
      181 gcgatggcgg aaggcgccgt tggcggcgct cggcggattg taggcgaagc gttcgtaagc
      241 gacgtcgtgc gtgttggcgt cctggagata ggcgcggact tcgagctggt cgaagagcgg
      301 ggcgttgccg tcgaagaggt aggcggcgcc gaggcggaag cgttgggtgg tgctgtcgag
      361 atcgagcccg gtggtggtgg tggcgccgga cataatctcc ttgttgttca cctgggcggt
      421 gagatcgcgg tcgaagtttt ccagggtgag ttcgaagcga tgcggcgcgg acggaagcca
      481 gaccagtttg gtgagaacgg cgtcggagga atagtcttcg ggattggggc ggacgatgcc
      541 gttgttttcg cgttcgtggc cttcgtggtg cgagtagacg acgagagcct gaagtgggcc
      601 gtgcgcggcg gcgaaggtgg cggagagaga cgtgccgtcg tcgacggagc ggtaagcggc
      661 tttggcgccg gcgtagatgc gtttgccgtt gagataatcg gcgggcgatt tggtggtgaa
      721 agtgacgacg ccgccgagcg cgtcgctgcc gtagagggcg gaggcggagc cttggagaat
      781 ttcgacgcgt ttgaagagct cgggatcgaa gtagtcgcgg ccggtgggtt cggagccgcc
      841 aagggcaaac tcgtcgggca cgcgcacgcc gtcgacctgg aggagcacgc gattaccctc
      901 gacgccgcgg atgttgatgc tttgagcgcc gccgcgctgg taggcgacgc ccgcgccgga
      961 gaaggcgaag ggaacgctga cgagcgtctc gccgcgaacg aactggtcga gcgtgacgcc
     1021 gtcgggttcg gtgaggtcga tcacgttgat ggtgccgggc gtggcgttga ccgggcgctc
     1081 ggtgc
//
LOCUS       DASPLT010001373         2391 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_303735, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001373 DASPLT010000000
VERSION     DASPLT010001373.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 2391)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2391)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2391
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_303735"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(201..575)
                     /locus_tag="VEA63_13965"
     CDS             complement(201..575)
                     /locus_tag="VEA63_13965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HYD85165.1"
                     /translation="MSQTTLLSSTDAQSLVLADAAQTSPSGIVSRTVLQSAELRVVLF
                     AFAEGQELTSHSSRRRALVQILDGSCEFFFAGAWQTLHAGAFLHMPPNHPHAVRAAAG
                     PFTMLLTLGNEAPTPSAAIESM"
     gene            856..1575
                     /locus_tag="VEA63_13970"
     CDS             856..1575
                     /locus_tag="VEA63_13970"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HYD85166.1"
                     /translation="MPTNHAQLRTTVLAATLRSCQLFSGLPASDLQTIASFAQLRSLA
                     KDAYLFREGDSSEGFYVVQKGAINVHRVSAAGKEQTIYVFRAGESLAEASMAVATGYP
                     ANARAIEASSVILVPKAPFLALLARRPDLGLRMLGSMSQHLRVLVGLVEDLTLKDVET
                     RVINWLVKRCPRDAQGEVAIELGMTKRVLAAELGTSSETLSRTFARLRSEKLLEVKGS
                     RLRVRDAAALRARFGRLLGEG"
     gene            complement(1761..2234)
                     /locus_tag="VEA63_13975"
     CDS             complement(1761..2234)
                     /locus_tag="VEA63_13975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD85167.1"
                     /translation="MRNDWVHALRSRLPQIRARWEALLHVEPVNTPLAYPDALVHLLD
                     WTLEQIFDGLANRALTLDSDRRPTCANYRHVCACGRNPLLTYFTAGEQAMREALVLTQ
                     AAAPALTALDRDRSLLALDAVFRNIARREIESFCGVCQYRDGGCPAEAAALRALH"
BASE COUNT          390 a          620 c          946 g          435 t
ORIGIN      
        1 gcggtggcga ttgaagctcc acgcaccaac ctccaggctc cagagaaaca tcaaaggagc
       61 tgaacggccg acgcggcaaa cgtagtctat ggcgtttaga gtggcttcaa aaattctgta
      121 gctgccacga gctggcgaag cgattgtcgt cccgcgtagc cgcgccggtt acgatgcgga
      181 ccgcgtccta atggacacgg ctacatcgat tcgatcgctg cggacggcgt gggcgcttcg
      241 ttgccgagtg tgagcagcat cgtgaagggg ccggcggcgg cacggacggc gtgcgggtga
      301 ttgggcggca tgtgcaggaa cgcgccggcg tggagcgtct gccaggcgcc ggcgaagaag
      361 aactcgcagc tgccgtcgag gatttgcacg agtgcgcggc ggcggctgct gtgtgacgtg
      421 agctcctggc cctcggcgaa agcgaagagg acaacgcgca gctcggcgct ttgcagcacg
      481 gtgcggctca cgatgccgga cggcgaggtt tgcgcggcgt cggcaagcac gagcgattgg
      541 gcgtcggtgg aagagagaag ggtggtttga ctcatgcgga gaacttaacg cgaatggcgc
      601 acgcgcgcat tgatcgcggt caagacggcg cgaagtagtg ccagcgggcg tgaggatttg
      661 gtttctggaa ggcaggccgt cgttcggcaa tgatcgcggc gagggcggcg gtgggcgttt
      721 gtcatctatt agatgacaaa cgttcggggg aggaacgctt gtggggattg gaaggcgacg
      781 tgcgtgggcc ggcgattttg ccgcacggag attttaggcg cgacgcgacg aaggggaggc
      841 gtttgaactt ggggcgtgcc gacgaatcat gcccagcttc gcaccacggt cctcgccgcc
      901 acgctgcggt cgtgtcagct tttctcgggc ttgccggcga gcgatttgca aacgatcgcg
      961 agtttcgcgc aattgcggtc gttggcgaag gacgcgtatc tgtttcgcga gggcgattcg
     1021 tcggaaggtt tttatgtggt gcagaagggc gcgatcaatg ttcaccgtgt gagtgcggcg
     1081 gggaaggaac agacgatcta tgtgtttcgc gcgggcgagt cgctcgcgga ggcgtcgatg
     1141 gcggtcgcga ccggttatcc ggcgaacgcg cgcgcgatcg aggcgtcgtc ggtgatattg
     1201 gtgccgaagg cgccgtttct ggcgctgctc gcccggcgac cggatctcgg gctgcggatg
     1261 ctcgggtcga tgagccagca tctgcgcgtg ttggtgggac tggttgagga cctgacgttg
     1321 aaggacgtcg agacgcgggt gatcaactgg ctggtgaagc gatgcccgcg cgacgcgcag
     1381 ggcgaagtgg cgatcgagtt ggggatgacg aagcgcgtgc tcgcggcgga gctcgggacg
     1441 agcagcgaaa cgctgtcgcg gacgtttgcg cgtttgcgga gtgagaagct gctcgaggtg
     1501 aaagggagcc ggctgcgggt gcgcgacgcg gcggcgctgc gggcgcggtt tgggcggctg
     1561 ttgggagagg ggtgagtgtg ggaggaaacc gcaccgtaac cggtgcggct acgtgtgagc
     1621 ggtgcggtcg cgtgcaaccg gtgcggctac gtgtaacctg tgcggttgcg cggtgggttg
     1681 gtgttgttcg tcgtgcgtta ttgtggggtg cgagcgcgca gtcggaggag caggcaggca
     1741 cggaggcctg ccgctacggg ttaatgcagc gcgcggaggg cggccgcttc ggcggggcag
     1801 ccgccgtcgc gatactggca gacgccgcag aacgattcga tttcgcggcg cgcgatgttg
     1861 cgaaagaccg cgtccagcgc gaggagcgat cggtcgcgat cgagtgcagt cagagccggc
     1921 gcagcggctt gcgtgaggac gagcgcttcg cgcatggctt gctcgccggc agtgaagtaa
     1981 gtgagcagcg ggttgcggcc gcaggcgcag acgtggcgat agttcgcaca tgtcgggcgg
     2041 cggtcgctgt cgagcgtgag ggctcggttc gcgaggccgt cgaaaatttg ttcgagcgtc
     2101 cagtcgagca ggtgcacgag cgcgtccgga taagcgagcg gcgtgttgac cggctcgacg
     2161 tgcagcagcg cttcccaacg cgcgcggatt tgcggcagtc gggaacgcaa ggcgtgcacc
     2221 cagtcgtttc gcatggcgcc actatgcccg aacggttcgc atcgtgctgc gcgtgccttg
     2281 gcgaaggttg cgcatggttt gatgcggcga atttgcgtcg ccgcgctgcg gccggttacg
     2341 cgggatcggc gggcgtctgg ccttgcgttt ccacccagcg gacggcgtgg c
//
LOCUS       DASPLT010001374         2705 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_220879, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001374 DASPLT010000000
VERSION     DASPLT010001374.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 2705)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2705)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2705
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_220879"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..369)
                     /locus_tag="VEA63_13980"
     CDS             complement(<1..369)
                     /locus_tag="VEA63_13980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD85168.1"
                     /translation="MCGSNLSRRAGLQPALDTSALPPCRAGSPDPAASRATSRFTSRP
                     FAPHLSTLLLAALVLLPACAKKNATPNGSADAARNASADILRISQRNEPADLDPATAS
                     LPDEFFIIRALAEGLLVPSAD"
     gene            complement(344..1978)
                     /locus_tag="VEA63_13985"
     CDS             complement(344..1978)
                     /locus_tag="VEA63_13985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HYD85169.1"
                     /translation="MPLSHSLLRLLSLFAAIGSLGFFSACAPRETPVDRGNREQILHR
                     GLSHDLADLDPHLATQSSDYIVLSALFEGLVAEDPVDLHPIPGVAGRWEISPDGLTYT
                     FFLRENARWSNGDPVTAHDFVASWRRMLTPAFGADYANLLFILQRAEAFNKGQATAEQ
                     VGVSAPDARTLRVTLEHPSPAFLSLLTNPAFYPVHLASIAQHGGTADRSNPWARPNRL
                     VGNGPFDLTAWDTGQKIVVEKSPTYWDHATVRLNAIHFHAIDSLDAEERAFRARQLHV
                     TDAIPPGKIDVYRRDSPHLLRIDPLLGTYFYRLNTTRPFLSDARVRRALALAVDRTAI
                     VDQILRGGQLPAHGFIPPGTAGYTPASSLPTDFEAARKLLADAGYPGGKGLPAFELLF
                     NNSEAHRTIAESIQEMWRRELGIEARLSNQELKSTLEARRAGNYEIMRSVWIGDYVDP
                     ETFLDIWRSDSGNNYTGWKNANYDALLFAAARETDAAKRRALYQKAESLLLEGAPMIP
                     IYYYTHVFLIQPSVRGWHPTLLDHHPYKHVWLEPQP"
     gene            complement(2145..2624)
                     /gene="ispF"
                     /locus_tag="VEA63_13990"
     CDS             complement(2145..2624)
                     /gene="ispF"
                     /locus_tag="VEA63_13990"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HYD85170.1"
                     /translation="MTFRIGHGYDIHRLVPNRPLVLGGVRFDTDYGLDGHSDADCLTH
                     AICDAILGAAGLPDIGHFFPNTDPAFKNIDSQLLLQRVRTEVIKLGFDIVNIDASVIA
                     EKPKIYPRLAEMKATLARSAGLTVDEIGLKATTNEGVGDLGRALAIAAHAVALLKRS"
BASE COUNT          484 a          621 c         1116 g          484 t
ORIGIN      
        1 gtcagcggaa ggaacgagca ggccttcggc gagagcgcga atgatgaaga actcgtcagg
       61 gagcgaagcg gtggcggggt cgaggtcggc gggctcgttg cgctgactga tgcgaaggat
      121 gtcggcggag gcgtttcggg cggcgtcggc ggagccgttg ggcgtggcgt tctttttagc
      181 gcaggcgggg aggaggacga gggcggcgag gaggagggtg gagaggtggg gtgcgaaggg
      241 gcgggaggta aaacgcgagg tggcgcgtga ggcggcgggg tcgggagacc ccgccctaca
      301 tggggggagt gcggacgtat cgagggcggg ctgaagcccg gccctacggc tgaggttcga
      361 gccacacatg tttgtaggga tggtggtcga ggagcgtggg atgccagccg cgaacggacg
      421 gttggatgag gaagacgtgg gtgtaataat agatggggat catgggcgcg ccttccaaga
      481 gaagcgattc cgccttttgg tagagcgcgc gacgtttggc ggcgtcggtt tcgcgggcgg
      541 cggcgaagag gagcgcgtcg tagttggcgt ttttccagcc ggtgtaattg ttacccgaat
      601 cgctgcgcca gatatcgagg aaggtttcgg gatcgacgta atcgccgatc cagacggacc
      661 gcatgatctc gtagttgccg gcacggcgtg cttcgagcgt gctcttgagt tcctggttgg
      721 ataggcgggc ttcgatgccg agttcgcggc gccacatttc ctgaatggat tcggcgatgg
      781 tgcgatgggc ttcggaattg ttgaagagaa gctcgaaggc ggggaggcct ttgcccccgg
      841 ggtagccggc gtcggcgagg agtttacgcg cggcttcgaa gtcggtggga agggaactcg
      901 cgggcgtgta gccagcggtg ccgggcggga tgaagccgtg cgcggggagt tggccgccgc
      961 ggaggatttg gtcgacgatg gccgtgcgat cgaccgcgag agcgagggcg cggcggacgc
     1021 gggcgtcgct gaggaacgga cgcgtggtgt tgagccggta gaaataagtg ccgaggagtg
     1081 gatcgatgcg aaggagatgc ggcgaatcgc ggcggtagac gtcaatcttg ccaggaggaa
     1141 tggcgtcggt gacgtgaagc tggcgcgcgc ggaaggcgcg ttcctcggcg tcgaggctgt
     1201 cgatcgcgtg gaagtgaatg gcgttgaggc gaacggtggc gtggtcccag taggtcgggg
     1261 atttttcgac gacgattttt tggccggtgt cccacgcggt gaggtcgaaa ggaccgttgc
     1321 cgacgaggcg gttggggcgg gcccacgggt tgctgcgatc ggcggtgccg ccgtgttgcg
     1381 cgatcgaggc gaggtgaacg ggatagaacg cggggttggt cagcagcgag aggaaggccg
     1441 gagacgggtg ctcgagcgtg acgcgcagcg tgcgggcgtc gggcgcggag acgccgacct
     1501 gctcggcggt ggcctggcct ttgttgaagg cttcggcgcg ttggagaatg aaaaggaggt
     1561 tggcgtagtc ggcgccgaac gccggcgtga gcatgcggcg ccaggaggcg acgaagtcgt
     1621 gcgcggtgac gggatcgccg ttggaccaac gggcgttttc gcggaggaag aacgtgtagg
     1681 tgagcccgtc gggagaaatt tcccagcgcc cggcgacgcc gggaatggga tggaggtcga
     1741 cgggatcttc ggcgacgagg ccttcgaaaa gcgcggagag gacgatgtag tcgctcgact
     1801 gcgtggcgag gtgcggatcg agatcggcga gatcgtgcga gaggccgcga tggaggattt
     1861 gttcgcggtt gccgcgatcg acgggggttt cgcggggcgc gcaggcggag aaaaatccca
     1921 gcgagccgat ggccgcaaaa aggctcagaa ggcgcaaaag ggaatgggac aggggcatcg
     1981 agtttttttt gcgcttttgg cgctactttg cggctaagac gtcaaaccgg ttgaaccacg
     2041 aatggacacg aaggcacacg aatgaaaggc accggcgggg gcgtgggttg gttcgagatt
     2101 cgggtgaatt cgtccattcg tggttcttca atgcacggct gcggctagct gcgtttgagg
     2161 agggcgaccg cgtgggcggc gatggcgagg gcgcggccga gatcgccgac gccttcgttg
     2221 gtcgtggctt tgagaccgat ttcgtcgacg gtgaggccgg cggagcgcgc aagcgtggcc
     2281 ttcatctcgg cgaggcgcgg gtagattttg ggtttctcgg cgatcacgct cgcgtcgatg
     2341 ttcacgatgt cgaaaccgag ttttatcacc tcggtgcgga cgcgctggag gaggagttgc
     2401 gagtcgatgt ttttgaaggc cggatcggta ttcggaaaaa aatgaccgat gtcagggagg
     2461 ccggcggcgc cgagaattgc gtcgcagatc gcgtgcgtga ggcagtcggc gtcggagtgt
     2521 ccatcgaggc cgtaatcggt gtcaaagcgc acgccgccga gaacgagcgg gcggttgggg
     2581 acgaggcggt ggatgtcgta gccgtggccg atgcggaaag tcatctagga aatctcaaac
     2641 gccaaaagga atctgctcgc tcacgctcgt tgcgactggg tgagcgcggg tgtgagcatg
     2701 ggtgt
//
LOCUS       DASPLT010001375         1401 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_240671, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001375 DASPLT010000000
VERSION     DASPLT010001375.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1401)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1401)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1401
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_240671"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..437)
                     /locus_tag="VEA63_13995"
     CDS             complement(<1..437)
                     /locus_tag="VEA63_13995"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HYD85171.1"
                     /translation="MTTMRKLPVSVLVPIRNEESNLPRCLASVAWADQVFVVDSQSTD
                     ASEAIARRYGAEVVQFHFNGIWPKKKNWALENLPFRHDWVLILDADEVMPPDAEAEIA
                     DVVAHDGHGHAGYWINRRFMFMGRWLKHAYYPNWNLRLFKHRLG"
     gene            complement(583..1149)
                     /locus_tag="VEA63_14000"
     CDS             complement(583..1149)
                     /locus_tag="VEA63_14000"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007364089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HYD85172.1"
                     /translation="MKKRASYRKKDYPLHRIRRYLETGPIVLVSSARAEQRNIMTMGW
                     HMMMQFSPALFGCYIWEENHSFDLIRKSRECVINLPTVDLVDEVIGIGNTTGAEIDKF
                     ERFGLTPRPATKVGAPLIDECYANFECRLADSSLIKKYSLFIWEVVKAHVAISPKNPR
                     TLHYRGSGEFMVAGRTLNRRRSFKPQNL"
     gene            complement(1286..>1401)
                     /locus_tag="VEA63_14005"
     CDS             complement(1286..>1401)
                     /locus_tag="VEA63_14005"
                     /inference="COORDINATES: protein motif:HMM:TIGR02595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HYD85173.1"
                     /translation="VPEPSTYAAWGAALLVGFVAYRRFRAGKNVEALPAAA"
BASE COUNT          282 a          396 c          439 g          284 t
ORIGIN      
        1 ccgagccgat gtttaaaaag acgcaggttc caattcggat aatacgcgtg cttcagccaa
       61 cggcccatga acatgaaacg ccggttgatc cagtagccgg cgtggccgtg gccgtcatgc
      121 gccaccacgt ccgcgatttc cgcttccgcg tcgggcggca tgacctcatc ggcatcgaga
      181 atcagcaccc agtcgtgacg gaacggcagg ttttcgagcg cccagttttt tttcttcggc
      241 cagatcccgt tgaagtgaaa ctgcacgacc tccgcgccgt agcgacgcgc gatcgcctcg
      301 gaggcgtcgg tgctttggga gtcgacaacg aaaacctgat cggcccacgc gacagacgcg
      361 aggcaccgcg gcagattcga ctcctcgttg cggatcggca cgagaacgga gacgggaagt
      421 ttacgcatcg tagtcatcga cagcggcggc gcgcgaaggt gtcgcgttgg atttggttga
      481 ggggaaaagc ttaatttggc ggagcacgat gcctcacggg gctggctcgc tcgaatgtag
      541 caacaggcct gaccagctcg atgggacgtc gggctccacc ggctagagat tttgcggttt
      601 gaagctgcgc cggcggttga gggtgcgccc tgcgaccatg aattcaccgc tgccgcgata
      661 gtgcagcgtg cgtggatttt tcggactgat ggcgacgtgc gccttcacca cttcccagat
      721 gaaaaggctg tattttttga tcaggctgct gtctgccagc cggcattcga aattggcgta
      781 gcattcatca atgagaggcg cgccgacttt cgtcgcggga cgcggggtga ggccgaaacg
      841 ctcgaacttg tcgatctctg cgcccgtggt gttgccgatg ccgatgacct cgtcaacgag
      901 gtccacggtt ggaaggttga tcacgcactc gcgcgatttt cggatcaggt cgaaactgtg
      961 attttcctcc caaatgtagc acccgaaaag cgccggcgag aactgcatca tcatgtgcca
     1021 acccatcgtc atgatgttgc gctgttccgc ccgagccgaa ctcacgagga cgatgggtcc
     1081 cgtttccaga taccggcgga tccggtgcaa cgggtagtct ttttttcgat acgaagctct
     1141 cttcttcatg gcgttttttt cgacggcgag tgctcggcgc gaacgcagta aatcgtgacg
     1201 gagatagcga aaaaaaaggc cgtccgcgaa cggacggcct tgaaggggtg ggacgccacc
     1261 gaagcggtga tgtccacgtt caggtttaag ccgctgccgg gagcgcttcg acgttcttgc
     1321 cggcgcggaa gcgacggtaa gcgacgaaac ccaccagaag cgccgcgccc cacgctgcat
     1381 acgtggatgg ctcgggaacg g
//
LOCUS       DASPLT010001376         2471 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_16934, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001376 DASPLT010000000
VERSION     DASPLT010001376.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 2471)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2471)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2471
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_16934"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..928)
                     /locus_tag="VEA63_14010"
     CDS             complement(<1..928)
                     /locus_tag="VEA63_14010"
                     /inference="COORDINATES: protein motif:HMM:NF016220.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease"
                     /protein_id="HYD85174.1"
                     /translation="MTGDIPYPEAGEGRARAVREDDVERAALEWFAGLGWQTLHGSNL
                     GPDGPQQNGQEPRADWRAVVVRPRLMAALAKLNPALPQTALEQAARQIIADESQDPLE
                     NNRRFLKLLRDGVDVEVRDGNSARTLKARLIDATDPLANDFLAVQQFTIVGVDERRPD
                     IIAFVNGLPLAVLELKDPANAGATLRSAYNQLQTYRARIPALFRFNAALVISDGLDAK
                     IGSLTAGLDRFSPWRTIDGAAPSIALDLETLVRGVFDKAHFVELACFYQAYEIPDGAA
                     KAKKLAGYHQFHAVRKALAATERAAAPSGDRKA"
     gene            complement(925..2295)
                     /locus_tag="VEA63_14015"
     CDS             complement(925..2295)
                     /locus_tag="VEA63_14015"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013579.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HYD85175.1"
                     /translation="MTAATPTVAVKPNEWLRGALSSVCTTITKGTTPKDFVESDADSV
                     VNYIKSESLNYDGTIDETAFAKIDAATHEALKRSQLAENDILISIAGAKLGKCGSVQR
                     KHLPANTNQAVAIVRVDPRKAHWRFVEYCLRQRTLTQAINSGVAQSAQPNLNLTDLGR
                     IEFALPPLVEQRAIAAVLGALDDKIEANRRINATLEAMARALFKSWFVDFDPVRAKME
                     GRDLAMAPDIVALFPDAMVEGVRGLTPAGWDMRALRDFVSLQRGTTYQSALLDDEGPF
                     LLGLASIAPDGGFRSTSLRTYGGDCPEKMLLRPGDIYVSLKDVTQSGDLLGAVARVPK
                     SIPVGRLTQDTLKLIFDRNAIEPTYVYWALRQPQYRAYCRARSMGTTNLSLSREDFLG
                     YELPIPSEAEQRIVARALDAFDSRVELNLQINATLALTRDLLLPRLLSGALRVRDAQC
                     MVGDAA"
     gene            complement(2292..>2471)
                     /locus_tag="VEA63_14020"
     CDS             complement(2292..>2471)
                     /locus_tag="VEA63_14020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HYD85176.1"
                     /translation="IAAQGFVLTPGRFVGAADVVDDGEVFEEVFARLKSKLDAQTTEG
                     RRLDDEIARQLRRLG"
BASE COUNT          446 a          782 c          762 g          481 t
ORIGIN      
        1 ccgctttgcg atccccgctc ggggcggcgg cgcgttcggt ggcggcgagc gctttgcgca
       61 cggcgtggaa ttggtgatag ccggcgagct tcttggcttt cgccgcgcca tcggggattt
      121 catacgcttg gtagaagcag gcgagttcga cgaaatgcgc cttgtcgaac acgccgcgca
      181 ccagcgtttc gagatcgagc gcgattgagg gcgcggcgcc atcgatggtg cgccaaggcg
      241 agaagcggtc gagcccggcg gtcagcgatc cgatcttcgc atcgagcccg tcgctgatga
      301 ccagcgcggc gttgaagcgg aacagcgcgg ggatgcgcgc gcggtaggtt tgcagctggt
      361 tgtaggccga gcgcaacgtc gcgccagcat tggccgggtc tttcagctcc agcaccgcga
      421 gcggcaagcc gttgacgaag gcgatgatgt cgggtcggcg ctcatcgacg cccacgatag
      481 tgaattgctg gacggcgaga aaatcgttgg cgagcggatc ggtcgcatcg atcaggcgcg
      541 ccttcagcgt gcgcgcggaa ttgccgtcgc gcacctcgac atcgacgccg tcacgcaaca
      601 gcttgaggaa gcggcgattg ttttcgagcg ggtcctggct ttcatcggcg atgatctgac
      661 gcgcggcttg ctccagcgcg gtctgcggca gggccggatt gagcttggcg agcgccgcca
      721 tcagccttgg ccgcacgacc acggcgcgcc aatcggcgcg cggctcttgc ccattctgtt
      781 gcgggccatc cggcccgaga ttagagccgt gcagcgtctg ccagcccagc cccgcgaacc
      841 attcaagcgc tgcgcgctcg acatcatcct cgcgcacggc gcgggcgcgc ccctcacccg
      901 cttcaggata ggggatgtcg cccgtcacgc ggcgtctcca accatgcact gggcgtcgcg
      961 cacgcgcagc gcgccggaga ggagccgagg gagcagcaag tctctcgtaa gtgcgagcgt
     1021 ggcgtttatt tgcaggttca gctcgacgcg actatcaaac gcgtccagag cgcgcgcaac
     1081 gatccgctgc tcagcttccg aagggatcgg tagttcataa cccaagaagt cctcccggct
     1141 tagcgaaagg tttgttgtgc ccatacttcg ggctctgcag tacgcgcgat actgaggctg
     1201 tctgagcgcc caatagacgt acgtgggctc gatggcgtta cgatcaaaga tgagcttgag
     1261 cgtgtcctga gttagccttc cgaccggaat gcttttgggg acgcgagcca ccgctcccag
     1321 caagtcaccc gattgcgtta cgtccttcag cgaaacgtag atgtctccag ggcgcaacag
     1381 cattttctcg ggacagtcgc ctccgtacgt gcggagcgaa gtggatcgaa accctccatc
     1441 gggtgcaatc gatgccaacc ccaggaggaa tgggccttcg tcgtccaaca gtgcactttg
     1501 ataagttgtg ccccgttgca acgacacgaa gtcgcggagc gcgcgcatgt cccaacccgc
     1561 cggcgtcaat ccacgcacgc cttccaccat cgcatccgga aatagcgcca cgatgtccgg
     1621 agccatagcg agatcccggc cttccatctt cgcgcgcacg ggatcgaaat cgacgaacca
     1681 gctcttgaac agcgcgcgcg ccatcgcctc cagcgtggcg ttgatgcgcc ggttcgcttc
     1741 gatcttatcg tccagcgccc ccaacaccgc cgcgatcgcg cgctgttcca cgagtggggg
     1801 aagggcaaac tcgatgcgcc caaggtcggt cagattcaga ttgggctgcg cggactgggc
     1861 gacccctgag ttgatcgctt gcgtgagagt ccgctgacga aggcaatact cgacaaatct
     1921 ccagtgtgct ttcctcggat cgacgcgcac tattgcaacg gcttgattgg tattggcggg
     1981 caagtgtttg cgctgaacac ttccgcactt tcccagcttc gcaccagcga tcgaaatcag
     2041 aatgtcgttc tccgccaact gggagcgctt cagcgcctca tgcgtagctg cgtcaatctt
     2101 ggcaaaggcg gtctcatcaa ttgtcccatc gtagtttagg gattctgatt tgatgtagtt
     2161 cacgacgctg tccgcgtctg actcaacgaa gtccttgggt gtcgttccct tggtaatcgt
     2221 cgtgcagacg ctcgacagag ccccacgaag ccattcgttt ggtttgactg cgactgttgg
     2281 ggttgcagca gtcaacccaa cctccgtagc tgccgcgcga tctcatcatc cagcctgcgc
     2341 ccctcggtcg tctgcgcgtc tagcttcgac ttgagccgcg cgaacacctc ctcgaagact
     2401 tcgccgtcgt ccaccacatc cgccgcgccg acgaagcgcc ccggcgtcag cacaaagcct
     2461 tgcgcggcga t
//
LOCUS       DASPLT010001377         1756 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_40664, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001377 DASPLT010000000
VERSION     DASPLT010001377.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1756)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1756)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1756
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_40664"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..732
                     /locus_tag="VEA63_14025"
     CDS             <1..732
                     /locus_tag="VEA63_14025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HYD85177.1"
                     /translation="AEHWTEPDEGIWEIRGPRRHFTHSKVMAWVALDRAIKSAETFSL
                     SGDVQRWVAVRDEVHAEVCARGYHEGRGVFTQYYGSEQLDASLLMMPLVGFLPASDPR
                     IVRTVLAIRDELTIDGLVNRYHPERSEKLDGLPPGEGTFLPCSFWLVDCLVLLERRDE
                     ARALFERLLALRTPLGLLAEEYDCGTKRLLGNFPQAFSHVALINSAQNLSRQAHPAEE
                     RSTGKPTAAKSDEADTQGAGRNASK"
     gene            complement(702..>1756)
                     /locus_tag="VEA63_14030"
     CDS             complement(702..>1756)
                     /locus_tag="VEA63_14030"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HYD85178.1"
                     /translation="LLVSASGLVPVKASSGHWAITEWFLHFSMQRSLSTHSLGIDVPP
                     LDDPALVLKGATHYEVGCRSCHGAPGERRPRVAQAMLPPPPALVPRIQKSNPKKLFQA
                     VKHGLKFTGMPAWPSQQRDDEVWAVVAFLLKLPELDAAAYRQLVGREPERRAPLEMPS
                     SDDSSRQHPATVIQSCVRCHGADGLGRDSAAFPKLAGQRREYLENALLAYADGRRHSG
                     IMEPIAAALDPAAIRQLATYYAQLARPAPAASRLGDDVSVERGRLIARDGIPSRRIPA
                     CIECHGPNGRRTKDAYPSLAGQPADYLVLQLELFKEGRRGGSPYAHLMEEVAPRLEPD
                     EMRDVARYFEALRPAP"
BASE COUNT          317 a          541 c          587 g          311 t
ORIGIN      
        1 gcggaacact ggacggagcc cgacgaaggc atttgggaaa ttcgcggccc gcggcgccat
       61 ttcacgcatt cgaaagtgat ggcgtgggtc gcgctcgacc gtgcgatcaa atccgccgag
      121 accttcagct tgagtggcga cgtgcaacgc tgggtcgcgg tgcgcgatga agttcacgcc
      181 gaagtttgtg cgcgcggata tcatgaaggc cgcggcgtgt tcacgcagta ttacggttcg
      241 gagcaactcg acgcgagcct gctgatgatg ccgctcgtcg gctttctccc ggcaagcgat
      301 ccgcggatcg ttcgcacggt gctcgcgatc cgcgacgagc tcacgatcga cgggctggtt
      361 aaccgctatc atccggagcg ctcggaaaaa ctcgacggtc tgccgccggg cgaggggacg
      421 tttctgccgt gctcgttctg gctcgtcgat tgcctggtgc tgctcgagcg tcgcgacgaa
      481 gcgcgggcgt tgttcgaacg actgcttgcc ctgcgcacgc cactcggatt gctggccgag
      541 gaatacgact gcggcacgaa acggctgcta ggaaattttc cgcaggcgtt ttcacacgtg
      601 gcgttgatca actcggcgca aaatctctcg cggcaggcgc atcccgcgga ggagcggagc
      661 acaggtaaac cgacggccgc caagtcggac gaagccgaca ctcagggagc ggggcgcaac
      721 gcttcgaaat aacgggcgac atcgcgcatc tcgtccggct cgagccgtgg cgcgacttct
      781 tccatcaagt gcgcgtaagg cgagccaccg cggcggccct ccttgaaaag ttcgagctgg
      841 agcacgaggt agtcggcagg ttgacccgcg agcgaaggat aggcgtcctt tgtgcgtcgt
      901 ccgttcggtc cgtggcattc gatacatgct ggaatccgac gcgacggaat gccgtcgcgc
      961 gcgatgagcc gtccccgttc gacggatacg tcgtccccca accgcgaagc cgcaggagcg
     1021 gggcgggcga gctgcgcata gtaggtagcg agttgacgaa tggccgctgg gtcgagcgcc
     1081 gccgcgatcg gctccatgat tccgctgtgt cgcctgccgt cggcataggc gagcagcgcg
     1141 ttctcgagat attcacgacg ttgtccggcc agtttcggga aagccgcgct gtctcggccc
     1201 aggccatcgg cgccatggca gcgcacgcag ctttgaatca cggtagccgg gtgctggcgt
     1261 gaggaatcat cggacgacgg catttcaagt ggtgccctcc gctccggctc gcgcccgacg
     1321 agctggcgat acgcggccgc gtccagctcc ggcaatttca acaagaacgc caccactgcc
     1381 cacacttcgt cgtcacgctg ttgcgacggc catgcgggca tgccggtgaa cttcaggccg
     1441 tgcttcaccg cttgaaacaa tttctttgga ttcgatttct ggatacgcgg cacgagcgcc
     1501 ggtggcggcg gcagcatcgc ctgcgcgacg cgcggtcgac gttcgccggg agcgccatgg
     1561 caggagcggc agccaacctc gtaatgcgta gcgcctttca gcacgagcgc aggatcgtcc
     1621 agcggcggca catcgattcc gaggctgtgc gtggagagcg agcgttgcat cgagaaatgc
     1681 aaaaaccact cggtgatcgc ccagtggccc gagctcgctt tgacgggaac gaggccgctc
     1741 gcggacacga gcagcg
//
LOCUS       DASPLT010001378         2641 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_159371, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001378 DASPLT010000000
VERSION     DASPLT010001378.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 2641)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2641)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2641
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_159371"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            96..473
                     /gene="rplL"
                     /locus_tag="VEA63_14035"
     CDS             96..473
                     /gene="rplL"
                     /locus_tag="VEA63_14035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007654177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HYD85179.1"
                     /translation="MSNITKDQVIEWLSAQSILDLAALVKDLEGKWGVTAAVAAAPAA
                     AGAAPAAAAVEQTEFTVVLKEAGANKIGVIKEVRAITGLGLKEAKDLVEGAPKPVKEN
                     VSKADSEEIKKKLEAAGAKVELK"
     gene            641..>2641
                     /gene="rpoB"
                     /locus_tag="VEA63_14040"
     CDS             641..>2641
                     /gene="rpoB"
                     /locus_tag="VEA63_14040"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HYD85180.1"
                     /translation="MADRTHSERINFGKLREVLQPPNLIEIQITSYLDFLQKGTPEKQ
                     RKPQGLEAVFREVFPILSYDERLTLEYVSYTLGDAKNTEIECLREGITYSVPLYVKLR
                     LREEDFIKDEEIYMGEIPMVTERGSFIINGAERVVVSQLHRSPGIAFEVTPHPNGKPL
                     HSFRIIPDRGTWLEVQFDNNDLLYVYLDRRRRRRKFLITTLLRAIGYSSDIDLLNLFY
                     EIKDLKVSKALDMENVSTLVLVEDAIDAQKGVVLARAFEPLTKAIVRTFEKHEITTLR
                     VIDTAADEGAIIRALKKDPTRNEEEALKEIYKRLRPGEPPTTANAKALLKRLFFDPKR
                     YDLGRVGRYKVNQKLGLKVDLEQRILESGDIVAATKYLVRLKKGEGVVDDIDHLGSRR
                     VRTVGELLANQCRVGLSRTERLVRERMTMYDQSVDSITPQKLINPKALTTVIRDFFAR
                     SQLSQFMDQINPLAELTHKRRLSALGPGGLNRERAGFEVRDVHPSHYGRICPIETPEG
                     PNIGLINSLSTYARVNEFGFIETPYRLVKEGRATEKIDYLTADQEEGKVIAQANSELD
                     EKGNFIGKVTVRKDGEFLEVPAADVNLMDVSPKQVISIAAGMIPFLEHDDANRALMGA
                     NMQRQGVPLLQAEAPFVGTGIEERVARDSKIVAVADEAGIVAS"
BASE COUNT          542 a          857 c          745 g          497 t
ORIGIN      
        1 gcgagccggg aagaaaacat caatccaggt cggctaggag atacgtctct taaacgcgtt
       61 tccactgaaa ccgctatccg cctcaggaga ttaccatgag caacatcacc aaagaccaag
      121 tcatcgaatg gctgtccgcc cagtccattc tcgatctcgc cgccctcgtt aaagatctcg
      181 aaggcaagtg gggcgtcacc gccgccgtcg ccgccgcgcc tgccgctgcg ggtgctgcgc
      241 cggccgctgc cgccgtcgag cagaccgagt tcaccgtcgt cctcaaggaa gccggcgcga
      301 acaagatcgg cgtcatcaag gaagtccgcg cgatcactgg cctcggcctc aaggaagcta
      361 aggacctcgt cgaaggcgcg ccgaagccgg tcaaggagaa cgtctccaag gccgactccg
      421 aggaaatcaa gaagaagctc gaggccgccg gcgcgaaggt cgagctcaag taagacccgt
      481 tggcgttttt actcgcatat cttccgatta gttcgggaca ggccgtgttt caccgcggcc
      541 tgtcctttgc cttttctctg tttgttcttt tcgtccgttt ttcgcgccat tccgctaccg
      601 ttgcgggtga gcgcgccgtc tgactttatc cgggaatccc atggccgatc gcacccattc
      661 tgaacgtatc aacttcggca agctccgcga agtgctccag ccgccgaatc tcatcgagat
      721 tcagatcacg tcctatctcg acttcctgca aaagggcacg cccgagaagc agcgcaagcc
      781 gcagggcttg gaagcggtct tccgcgaagt gtttcccatc ctctcctacg acgagcggct
      841 gacgctcgaa tacgtttcct acacgctcgg cgacgcgaag aacaccgaga tcgagtgcct
      901 ccgtgaaggc atcacgtatt cggttcctct ctacgtgaag ctccgcctgc gcgaggaaga
      961 cttcatcaag gatgaagaga tttacatggg cgagatcccg atggtgaccg agcgcggctc
     1021 gttcatcatc aacggcgccg agcgcgtcgt ggtttcccaa ctccaccgtt cgcccggcat
     1081 cgcgttcgaa gtgacgccgc atccgaacgg caagccgctc cattctttcc gcatcattcc
     1141 cgatcgcggc acctggctcg aagttcagtt cgacaataac gacctgctct acgtttacct
     1201 cgatcgccgc cgccgccgcc ggaagttcct catcacgacg ctgctccgcg ccatcggcta
     1261 ttcgagcgac atcgacctcc tgaatctctt ctacgagatc aaggacctga aggtctccaa
     1321 ggcgctcgac atggagaacg tctccacgct cgtgctcgtc gaggacgcga tcgatgccca
     1381 gaagggcgtc gtgctcgcgc gcgcgttcga accgcttacg aaggcgatcg tccgcacgtt
     1441 cgagaaacac gagatcacca cgctgcgcgt gatcgacacc gcggccgacg aaggcgcgat
     1501 catccgcgcg ctgaagaagg atccgacccg caacgaagag gaagcgctca aggaaatcta
     1561 caagcgcctt cgtcccggcg agccgccaac cacggcgaac gccaaggctc tcctcaagcg
     1621 tttgttcttc gatccgaagc gctacgacct cggccgcgtt ggccgttaca aggtcaacca
     1681 gaagctcggc ctcaaggtcg acctggagca gcgcattctc gaatccggcg acatcgttgc
     1741 cgcgacgaag tatctcgttc ggctcaagaa gggcgaaggc gtcgtcgacg atatcgatca
     1801 cctcggctcc cgtcgcgtcc gcaccgtcgg cgagttgctc gccaaccaat gccgcgtcgg
     1861 cctcagccgc acggagcgtc tcgttcgcga gcggatgacg atgtatgacc agagcgtcga
     1921 ttcgatcacg ccgcaaaagc tgatcaaccc gaaggcgctc acgacggtga tccgcgattt
     1981 cttcgcgcgt tcgcagctct cgcagttcat ggaccagatc aacccgctcg ccgagctcac
     2041 gcacaagcgt cgcttgtccg cgctcgggcc tggtggtttg aaccgcgaac gtgccggctt
     2101 cgaagtgcgt gacgttcatc cgtcgcacta cggccgcatt tgtccgattg aaacgccgga
     2161 aggtccgaac atcggtctga tcaactcgct ctccacttac gcgcgcgtca acgaattcgg
     2221 cttcatcgag acgccgtatc gtctcgtgaa ggaaggtcgt gcgaccgaga agatcgacta
     2281 cctcaccgca gaccaggaag aggggaaagt aatcgcgcag gcgaattccg aactcgacga
     2341 aaagggcaac ttcatcggca aggtcacggt tcgtaaggac ggcgagtttc tcgaagtccc
     2401 cgccgcggac gttaacctga tggatgtttc gccgaagcag gtcatctcga tcgccgcggg
     2461 catgattccc ttcctcgagc acgacgatgc gaatcgcgcg ctcatgggtg cgaacatgca
     2521 acgccaaggc gtgccgctgc tccaagccga ggcgccgttc gtcggcaccg gcatcgaaga
     2581 gcgcgtcgcg cgcgactcga agatcgtcgc tgtcgccgat gaggcgggca ttgtcgcttc
     2641 c
//
LOCUS       DASPLT010001379         1130 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_282072, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001379 DASPLT010000000
VERSION     DASPLT010001379.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1130)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1130)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1130
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_282072"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1026
                     /locus_tag="VEA63_14045"
     CDS             <1..1026
                     /locus_tag="VEA63_14045"
                     /inference="COORDINATES: protein motif:HMM:NF014237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HYD85181.1"
                     /translation="AMRFTRFLLLFFPVLSALAAGHAPANPRANPRAHALLEYIASLE
                     PRTEKRMLSGQFTDFGDSGTLAIIEKIHAKTGEWPAIAGFDYVDFRSGGLATKVSNQS
                     AIAYWKAGGIVTVGVHLYNPANPKGGGLRDKGVDLNDLLDESTDTHRRWMQELDTLAA
                     GLQELEDAGVPVLWRPFHEMNGAWFWWGAKPPADFIKVWRHMFDYFTHTKKLDNLLWV
                     YGPNHMANTGDYYPGDAYVDLVGFDAYTDHIDPQHIKGYDALAALPKPFGFGEYGPHG
                     AQNPPGNYDYRRFIEGVQKHFPRTTFFMSWNAKWSLAENEHVAELLAHPWIVNRDDLP
                     ASITAER"
BASE COUNT          234 a          390 c          299 g          207 t
ORIGIN      
        1 gcaatgcgct ttactcgatt ccttcttcta tttttccccg tcctctccgc gctcgctgcc
       61 ggtcacgcgc ccgcgaatcc ccgggcaaat ccgcgagccc acgcgctgct cgaatatatc
      121 gcgagcctcg aaccgcgcac cgagaaacgc atgctgtccg gccagttcac cgatttcggc
      181 gacagcggca cgcttgcgat catcgagaaa attcatgcga aaaccggcga gtggccggca
      241 atcgctggtt tcgactatgt ggattttcgc agcggaggcc tcgccaccaa ggtgtcaaac
      301 caaagcgcga tcgcctactg gaaagccggc ggcatcgtca cggtcggcgt tcacctctac
      361 aatcccgcga acccgaaagg cggcggcctc cgcgataagg gggtcgattt gaacgacctc
      421 ctcgacgagt ccaccgacac gcatcgccgc tggatgcagg agctcgacac gctcgcggcc
      481 ggtttgcagg agctcgagga cgccggcgtg cccgtgctct ggcggccatt tcacgaaatg
      541 aacggcgcct ggttctggtg gggcgcgaaa ccgcccgccg acttcatcaa agtttggcgg
      601 cacatgttcg actatttcac ccacaccaag aagctcgaca acctgctctg ggtttacggg
      661 ccgaatcaca tggccaacac cggcgactac tatcctggcg acgcctacgt cgatctcgtc
      721 ggtttcgatg cctacaccga tcacatcgat ccgcagcaca tcaaaggcta cgacgcgctc
      781 gccgcgctgc cgaaaccttt cggcttcggc gaatatggcc cgcatggtgc ccagaatccg
      841 ccgggcaact atgactatcg ccgcttcatc gaaggcgtgc agaaacattt tccgcgcacc
      901 acgtttttca tgagctggaa cgcaaaatgg agtctcgccg aaaacgaaca cgtcgccgaa
      961 ctcctcgccc acccgtggat cgtcaaccgc gacgatctcc ccgccagcat caccgccgaa
     1021 cgttaagatc gcccgctaac acttcgaaag cgaacgccgc tccacaccaa agtagggcgg
     1081 gcttcagccc gctccctcag cgagcccgct gattacagcg cccgtttcgt
//
LOCUS       DASPLT010001380         1020 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_16965, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001380 DASPLT010000000
VERSION     DASPLT010001380.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1020)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1020)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1020
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_16965"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            319..>1020
                     /locus_tag="VEA63_14050"
     CDS             319..>1020
                     /locus_tag="VEA63_14050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA degradosome polyphosphate kinase"
                     /protein_id="HYD85182.1"
                     /translation="MKRVKKYNRLTAATGKSIARPANNQGIRPQYANREQSWLNFNRR
                     VLEQAQSEANPLLERAKFLAIVSSNLDEFFEIRVAGLIQQTDAGTGELSLDGLTPREQ
                     LKRVHNEANALVDEQYRCWHEQLVPALAKQNIVFRAAPQLTDPEAAWVRHYFRTQVLP
                     ILTPLAVDQAHPFPQIVNKTLNVLLSLDNPDTPETEKTVVILPVPRILPRIVQIEPDG
                     DGAERYIFLSEIIKLC"
BASE COUNT          204 a          376 c          242 g          198 t
ORIGIN      
        1 ctcgccgcga gcattttcca tttcggcacc tacacgatcg cccaagccaa gaattttctc
       61 ggccagcgcg gcgtccccgt ccgcctataa gtaagtaggg cgggcttcag cccgcctttt
      121 ttcgtcgccg cgtccgggtg atgggcttca gcccgcctta ctcttgctta cgcatcgccg
      181 tcgcctccgc gcctcgtccg gccttgccgt tgctattgcg gcgcgccatg taacaatttg
      241 gtgacgccgg ccattccgct cgtttcgcca gaccgcgttt cgtaacttaa ccgtctcctt
      301 cgcttaaccc gctctctcgt gaaacgcgtc aagaaataca accgcctcac cgccgcgacc
      361 ggaaaatcca tcgcgagacc tgccaacaac cagggtattc gcccgcagta tgcgaaccgc
      421 gaacagagct ggctcaactt caaccgccgt gtcctcgagc aagctcaaag cgaggccaac
      481 ccactcctcg aacgcgccaa gtttctcgcg atcgtcagct ccaatctcga cgagtttttc
      541 gaaatccgcg tcgccggcct cattcagcaa accgacgccg gcaccggtga actcagcctc
      601 gacggtctga cgccgcgcga acaactgaag cgcgtccaca acgaagccaa cgccctcgtc
      661 gacgagcaat accgctgctg gcacgaacaa ctcgtgcccg ccctcgcgaa acaaaatatc
      721 gtttttcgcg cggcaccaca gctcaccgat cccgaagcgg cctgggttcg ccattacttt
      781 cgcacgcaag tgctgccgat cctcacccca ctggccgtcg accaggcgca tccgtttccc
      841 cagatcgtca acaagacgct caacgtcctc ctctcgctcg acaatcccga cacgcccgag
      901 accgagaaga ccgtcgtgat cctgcccgtg ccgcgtattc tccctcgcat cgtgcagatc
      961 gagcccgacg gcgacggcgc tgaacgttac attttcctga gcgagatcat aaaactctgc
//
LOCUS       DASPLT010001381         3038 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_16973, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001381 DASPLT010000000
VERSION     DASPLT010001381.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 3038)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3038)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3038
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_16973"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1608
                     /locus_tag="VEA63_14055"
     CDS             <1..1608
                     /locus_tag="VEA63_14055"
                     /inference="COORDINATES: protein motif:HMM:NF036826.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HYD85183.1"
                     /translation="DGKVYVVWGYQGLQFAEMKSDLSDIKRETQRTLIEPRNGMGEGC
                     HFYKIDGRYYIISANYNPVGYMVCARADRPEGPYEITTISAEETFGVGNGWRLRGMGR
                     GGPGFELQEPRPTQMGAIPLHQGGIVQTPAGEWWGFSMMDHNSIGRLTCLSPVTWKDG
                     WPFFGLPGNPLRTPRTWLKPKTAAPYERTAPYERDDDFAGPKLNPVWQWNHVPVDSKW
                     SLEARSGFLRLQTQPAKNFWTANNTLTQRAVGPESIPTAELEISGLKPGDVAGLALLN
                     HPYAWIGVTRTDAGTMLTQFDELSGESATAPMPEGVARVWLRAECDFESEQAIFSFST
                     DGRTFAPLGRAFTMVFQLKTFQGVRYSLFAYNSTGAPGGHVDFDRFTVAEPQANGTGD
                     RIPIGKVITLTSLADDTRLVAWNGVLRPVPLRAPQASTAAARFRVLDRGQGRVALQAE
                     DGSGIVAVTGVGGLGDVRVQKSPGETASSFQWQDMLRDEVMLLSVVTQRHIHAEPQSG
                     GLASADSPGARPGRKDGTCFRWEEVSP"
     gene            1605..2627
                     /locus_tag="VEA63_14060"
     CDS             1605..2627
                     /locus_tag="VEA63_14060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HYD85184.1"
                     /translation="MSRAVRRAISFVVLSIALATGVDAATTHRSQIRIRDPFVLPDRA
                     TQTYYIYGTTTSGIFDGGIERKAVMVFKTKDLENWEDPVPVWEVPADHWGRETVWAPE
                     VHPYRGKYYLFATITATETLPTPPGRPQNVKRGTEILVADSPLGPFQPLGHGRQTPPD
                     WMALDGSLWIEDGVPYMVFCHEWAQITDGSFDLVRLSDDLSRAAGEPRLLFHASAAPW
                     VRCRGDIGELYQGKRYHAYVSDGNWLHRTKTGTLLMLWSSYGPGKYAVGIARSTTGSV
                     FGPWEQLPEPLWLDDGGHPMLFETFDGRRVMVIHQPNRQVERARFFEIEDLGDTLRIR
                     NEIGVR"
     gene            complement(2719..>3038)
                     /locus_tag="VEA63_14065"
     CDS             complement(2719..>3038)
                     /locus_tag="VEA63_14065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD85185.1"
                     /translation="LIADPRLWRRRAAGASLPEPYFLNIDTVEGYELILNESPEARRE
                     LPGNLIERAPVGTFDPTTLAFVPRDPALLERIGFQPIPVEQIGLQLDAWRRTVAPRFT
                     GQD"
BASE COUNT          571 a          868 c         1038 g          561 t
ORIGIN      
        1 gacggcaaag tgtatgtggt ttggggatac caaggcctgc agttcgcgga gatgaagtcc
       61 gacctgagcg acatcaaacg cgaaacgcag cgcacgttga tcgaaccgcg caacggcatg
      121 ggcgaaggtt gccatttcta caaaattgac ggccgctact acatcatcag cgcgaactat
      181 aatcccgtcg gttacatggt ctgcgcacgc gcggatcgac ctgagggtcc gtatgaaatc
      241 accaccatca gcgccgagga gacgttcggc gtaggtaatg gttggagact gcgcggcatg
      301 ggaaggggag ggcctggctt cgagttgcag gagccgcggc ccacgcagat gggtgcgatt
      361 ccgctgcatc agggtggaat cgtgcaaacg ccggccggcg agtggtgggg tttttccatg
      421 atggatcaca actccatcgg gcggctcacg tgtttgtcac cggtgacgtg gaaggatggc
      481 tggcccttct tcggacttcc gggcaatccg ctgcgcacgc cgcgcacgtg gttgaagccg
      541 aaaaccgcgg ctccgtatga acgaaccgca ccgtatgagc gcgatgacga tttcgccggg
      601 ccgaaactca acccggtgtg gcagtggaat catgtgccgg ttgattcgaa atggtcgctc
      661 gaagcgcgct ccggttttct gcgcttgcag acgcaaccgg cgaaaaattt ctggacggcg
      721 aacaacacgc tgacgcagcg cgcggtcggc ccggagtcga tcccgacggc ggaactcgag
      781 atttccggct taaagccagg agacgtcgct ggactcgcgc tgttgaatca tccttacgcg
      841 tggatcggtg tcacgcgcac ggacgcgggc acgatgctca cccagttcga cgagttgagc
      901 ggcgagagcg cgacggcgcc gatgcctgaa ggcgtcgcgc gggtgtggct gcgcgcggag
      961 tgcgatttcg agagcgagca ggcgatcttc agcttcagca ccgacggccg gacattcgcg
     1021 ccgctcggcc gcgcgttcac gatggtgttt caactgaaga cattccaagg cgtgcgctat
     1081 tcgctgttcg cgtataactc gaccggcgcg ccgggcggac atgtggactt cgatcgcttc
     1141 acggtcgcgg agccgcaggc gaatgggacg ggagaccgca tccccatcgg caaagtgatc
     1201 acgctgacga gtctcgctga cgatacgcgg ctcgtggcgt ggaacggtgt cttgcggccc
     1261 gtgcccttgc gtgctccgca ggcctccacg gcggcggcgc gattccgtgt gctcgaccgt
     1321 gggcagggac gcgtcgcatt gcaggcggaa gatggtagcg gtattgtcgc ggtcactggc
     1381 gtcggcggac tcggcgatgt gcgggtgcag aaatcgccag gcgaaaccgc gtcgagtttc
     1441 caatggcagg atatgctgcg cgacgaagtc atgctgctct cggtcgtcac ccaacggcac
     1501 attcacgcgg agccgcagag tggcggcttg gcgtcggcgg attcgccggg agcgcggccc
     1561 ggacgtaagg acgggacgtg ttttcgctgg gaggaagttt ctccatgagc cgggcggtgc
     1621 gacgtgcgat atcttttgtc gtcctctcga tcgcgctcgc aaccggagtt gacgcggcca
     1681 cgacccatcg ctcgcagatt cgcattcgcg atccctttgt gctgccggat cgcgccacgc
     1741 agacttacta catctacggc accacgacgt cggggatctt cgatggtggg atcgagcgca
     1801 aagcggtgat ggttttcaaa acgaaggatc tcgaaaactg ggaagatccg gttccggtgt
     1861 gggaagtgcc cgcggatcat tgggggcgcg aaacggtgtg ggcgcccgag gttcaccctt
     1921 accgcggaaa atactatttg ttcgcgacca tcacggcgac ggaaacgctg cccacgccgc
     1981 ccggccggcc gcagaatgtg aaacgcggca ccgaaattct cgtcgccgat tcaccgttgg
     2041 ggccgtttca accgctcggt cacgggcggc aaacgccgcc ggattggatg gcgctcgacg
     2101 gcagcttgtg gatcgaagac ggcgtgccct acatggtgtt ttgccacgag tgggcgcaga
     2161 tcaccgacgg cagcttcgac ctcgtgcgct tgagcgatga cctgtcgaga gcggcgggcg
     2221 agccgcgact gctctttcac gcctccgcgg cgccgtgggt tcgttgtcgc ggcgacatcg
     2281 gcgaactgta tcaaggaaaa cgataccacg cttacgtcag cgacggcaac tggctgcacc
     2341 gcacgaaaac cggcactctg ctcatgctgt ggtccagcta tgggcccggc aaatatgcgg
     2401 tgggaatcgc gcgttcgacg acgggcagcg tgtttggccc atgggagcag ttgccggagc
     2461 ctctgtggct cgacgatggc gggcatccga tgctcttcga aacctttgac ggacgaaggg
     2521 tcatggtgat ccatcagcca aaccggcagg tggagcgggc gcgctttttc gagatcgagg
     2581 atctcggcga tacgcttcgc attcgaaacg aaatcggcgt ccgctaaggg tcgggacgat
     2641 cgagcccctg caatggcgtg cccatcgctc acgggtcggt gcggagctgc agagaaggaa
     2701 cgttgagtga atatttcctc agtcctgacc cgtgaagcgc ggggcgaccg ttcggcgcca
     2761 ggcgtcgagt tggaggccga tttgctcgac ggggatcggt tggaaaccga tgcgctcgag
     2821 caacgcggga tcgcgaggaa cgaaggcgag cgtggtggga tcgaaggtgc cgacgggcgc
     2881 gcgctcaatc aggttgccgg gtagttcgcg acgggcctcg ggcgattcgt tcaagatcag
     2941 ttcgtatccc tcaaccgtgt cgatgttgag aaagtaaggc tcgggcaggc tggctcccgc
     3001 ggcgcgacgg cgccaaagcc tcggatccgc gatcaggt
//
LOCUS       DASPLT010001382         1024 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_159409, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001382 DASPLT010000000
VERSION     DASPLT010001382.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1024)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1024)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1024
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_159409"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1024
                     /locus_tag="VEA63_14070"
     CDS             <1..>1024
                     /locus_tag="VEA63_14070"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HYD85186.1"
                     /translation="ALDNSGGAILAPAGYIAALSPSLRALLGPNGEYTSAFFNAGGRP
                     LTVKLDGRTVLSDPNDFADSRDLLYFADLVNQRTAGRTWKNQFLLDWLETDKRSSYGY
                     AYATEQLVLENKFTVEQTILAGPLRRLVYGGALRYAWAHQLQDYSAEPFSRRDISQPE
                     ISANSIVLTGAQRPQTGDTRNLWSQGDDTEFTSAGLFGVGTFQFTERFSTIASLRVEG
                     ARFSVHTPSAFERSPTRGRKIAQGGKNYAMVSLNPLFKLTPTVSLYAALQRGTALNPS
                     QGGTITSEDNFGETDLAEVGVKTSLLDGRLYTSFAVYHTTLARMNAVTQNPFGLRSKG
                     VELEAT"
BASE COUNT          191 a          388 c          267 g          178 t
ORIGIN      
        1 ccgccctcga caactccggc ggcgccatcc tcgcgcctgc gggctacatc gccgcgctct
       61 caccgtctct ccgcgccctc ctcggcccca acggcgaata cacctcggcg tttttcaacg
      121 ccggcggacg tccgctcacc gtgaagctcg acggccgcac cgtgctctcc gaccccaacg
      181 acttcgccga ctcgcgcgac ctgctctatt tcgccgacct cgtgaaccag cgcaccgccg
      241 gtcgcacgtg gaaaaaccag tttctgctcg actggctcga aaccgacaaa cgctcctcct
      301 acggctacgc ttacgccacc gaacagctgg tgttggaaaa caagttcacc gtcgagcaaa
      361 ccatcctcgc cggaccgctg cgccgcctcg tttacggtgg cgcgctgcgc tacgcctggg
      421 cgcatcagtt gcaggattat tccgcggagc cgttttcgcg ccgcgacatt tcgcaaccgg
      481 aaattagcgc aaactccatc gtcctcaccg gcgcgcagcg tcctcaaacc ggtgacacac
      541 gcaacctctg gtcgcaaggc gacgacaccg agttcacctc cgccggcctt tttggcgtcg
      601 gcacgtttca gtttacggaa aggttcagca ccatcgcgtc gctgcgcgtc gaaggcgcgc
      661 gcttcagcgt gcacacgccc tccgccttcg agcgcagccc cacgcgcggc cggaagatcg
      721 cacaaggcgg caagaactac gcgatggtta gtctcaatcc gctctttaaa ctcacgccga
      781 ccgtctcgct ttacgccgcc ttgcaacgcg gcacggcgct caatccgtcg caaggcggca
      841 cgatcaccag cgaagacaac ttcggcgaaa ccgatctcgc cgaagtcggc gtgaaaactt
      901 cgctgctcga cggtcgcctc tacacgtcgt tcgccgttta tcacaccacg ctcgcgcgca
      961 tgaacgccgt cacgcaaaac cccttcggcc tccgcagcaa gggcgtcgaa ctcgaggcca
     1021 cgtg
//
LOCUS       DASPLT010001383         1092 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_55994, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001383 DASPLT010000000
VERSION     DASPLT010001383.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1092)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1092)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1092
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_55994"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(112..483)
                     /locus_tag="VEA63_14075"
     CDS             complement(112..483)
                     /locus_tag="VEA63_14075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD85187.1"
                     /translation="MKRFGYVFDPLCLAACALYALNRFWLHGHVGGAFFHGYFNDLLL
                     IPAALPLALWLQRKLGLRADDARPRWREIGLHLAAWALMAEAVAPHLLAHVTGDWRDL
                     VAYSAGALAAGCWWQGAATLG"
BASE COUNT          238 a          297 c          383 g          174 t
ORIGIN      
        1 gatgtttccg ataggcggcg ggtgggtcga aaattgtctg caagcctatc ggcgggctcg
       61 ggggccttga gcggagtcga tggggcggga gccatcgctt tttgcgcctt cttatccgag
      121 cgtcgcggcg ccctgccacc aacaacccgc ggcgagtgcg ccggcggaat aggcgacgag
      181 gtcgcgccag tcgccggtga cgtgggcgag caagtgaggc gcgacggctt cggccatgag
      241 cgcccacgcg gcgaggtgaa ggccgatctc gcgccatcgc gggcgggcgt cgtcggcgcg
      301 gaggccgagt ttgcgttgca gccagagcgc gagcgggagg gcggcgggga tcagcagcag
      361 gtcgttaaaa tagccgtgga aaaacgcgcc gccgacgtgg ccgtgcaacc agaaccggtt
      421 gagcgcgtag agcgcgcacg cggcgaggca gagcggatcg aagacgtagc cgaaacgttt
      481 cacaacaagg ccgcgatcag gaggccgctg ccgagggcga gcagcaggcg gaggacgaac
      541 ggcgggagat agggaggaaa tgacatgacg gcgaaattga atgccggacg aggtgctagg
      601 caaatacgtt gaacggactc atgggctcac gactgcacct aaaaggacgg gtggcgggtc
      661 acgcgcgcca gcttatccga cgaagctttc agcgtcaccg gagcaattca gtaggaaacc
      721 accgatgaac acggatggac acggattgaa aaccctttcc acaacgaaag ggtcattgaa
      781 aagactcact cttcggggaa gagaaacata tcgcattagg ctcctgagcc acgaggccgt
      841 atccgtgttt atcggtgttc atccgtggtt cgattgcagg gttccggctt agcgcggctc
      901 aataacatca tagccacgaa tgtagcggca ggcctccgtg cctgcttggg gcgataacgt
      961 tgaaaggcag gcaggctgag acgcctgccg ctacggctaa acggccacag cttttcggag
     1021 tatgctccag cccggaaaat tcacacctgt ggcgaaacgc gtgagcgttt cgtctctcgc
     1081 ccggacgggg cg
//
LOCUS       DASPLT010001384         1670 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_40698, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001384 DASPLT010000000
VERSION     DASPLT010001384.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1670)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1670)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1670
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_40698"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..250)
                     /locus_tag="VEA63_14080"
     CDS             complement(<1..250)
                     /locus_tag="VEA63_14080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HYD85188.1"
                     /translation="MKILAVEDDLVSRAVLRQALHRLGHEVLEAGDGESAWDLLQREP
                     VRVVVSDWMMPRLDGLELCRRVRGRIGGEYVYFILLTSS"
     gene            complement(339..743)
                     /locus_tag="VEA63_14085"
     CDS             complement(339..743)
                     /locus_tag="VEA63_14085"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HYD85189.1"
                     /translation="MAAILVIDAYDGFREAMSYCLPQFGHVALVAVDREEALRIAKEN
                     EVDVVLLDLGYRQQTGFLVCAALKRHERLKETPVILMGDTVTTGARERAQACGAETMI
                     AKPIEWPEFLALVDRLTPPATTRTPDDSRTDL"
     gene            complement(747..965)
                     /locus_tag="VEA63_14090"
     CDS             complement(747..965)
                     /locus_tag="VEA63_14090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD85190.1"
                     /translation="MQKNRVRRAGVSLLEDTRYHRIRLLSLLADVCRKIERLQSSVEA
                     RRACREVAASLRRLLANEENGRGVSRSE"
     gene            1037..1579
                     /locus_tag="VEA63_14095"
     CDS             1037..1579
                     /locus_tag="VEA63_14095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HYD85191.1"
                     /translation="MTLVITRDYSMRMDYTQLFRSLREAKSLTLEQLASAAAVHRNTV
                     VNIEGGRPVKFKTIALLMQKMGYAPSSPEMKSMALLWLEKVSGIPFSRAEPGSTAKKT
                     IETYRTPARQSAHQLCDAVIEESLSPAQIDVLLFAARHPEVMSIIESVRGFAQGFTPG
                     KRGKAFLQAAEDPATYGKKR"
BASE COUNT          354 a          598 c          423 g          295 t
ORIGIN      
        1 tgctgctcgt cagcaaaatg aaataaacat actcgccgcc gatccgcccg cgcacgcgcc
       61 gacacagctc cagcccgtcg agccgcggca tcatccaatc gctcaccacg acgcgcaccg
      121 gttcgcgctg caacagatcc cacgccgatt cgccatcgcc cgcctcgagc acttcatgtc
      181 ccaaccgatg cagcgcctgt cgcaggacgg cgcgggaaac aagatcgtct tcgacggcca
      241 ggattttcac tcggcggcga gcgttggccg gaaccccgcg cacaggcaat ggggaaaatt
      301 tccgggcatc ggcaaatttc tcatcccgcg aagtgtcgtc acaaatccgt tcgtgaatca
      361 tccggcgtgc gcgtcgtcgc cggcggcgtc agccggtcca ccaacgctaa aaattccggc
      421 cactcgatcg gcttcgcgat catcgtctcc gcaccacacg cttgcgcgcg ctcgcgtgcg
      481 cccgtggtca ccgtatcgcc catcaggatc accggcgtct ccttcaatcg ctcgtgccgc
      541 ttcaacgccg cgcacaccaa aaatcccgtt tgctgccgat accccaagtc gagcaacacg
      601 acgtcgactt cattttcctt cgcgatccgc aacgcctcct cgcgatccac cgccaccagc
      661 gcgacatgcc cgaactgcgg caggcaatac gacatcgcct cgcgaaaccc atcatacgca
      721 tcgatcacca ggatggcagc catacatcac tcgctcctgc tcacgccccg cccgttttct
      781 tcgttggcga gcagccgtcg caggctcgcc gccacctcgc gacacgctcg ccgcgcttcg
      841 accgacgact gcagccgctc gatctttcga cacacgtccg cgagcaacga cagcaggcga
      901 atacgatgat aacgcgtgtc ctccagtagc gagacgccgg ctcgtctcac gcggttcttt
      961 tgcatgagaa catattttgt agtcacctaa catttgtcaa ccaccgctcc cttggtgccg
     1021 ttccacctgc ggcacgttga cattggtaat cactcgcgac tactccatgc ggatggatta
     1081 cacgcagttg ttccgaagct tgcgggaggc gaaaagcctt actctggaac aactcgcctc
     1141 cgccgccgcc gtgcatcgca acaccgtcgt gaacatcgaa ggcggtcgcc cggtgaagtt
     1201 caaaacgatc gccctgctga tgcagaaaat gggctacgcg cccagttcgc ccgagatgaa
     1261 aagcatggcg ctcctctggc tcgaaaaagt cagcggcatt ccgttctcgc gcgccgagcc
     1321 tggctccacc gccaagaaaa cgattgaaac gtatcgcaca cccgcccgcc aatccgccca
     1381 ccaactctgc gacgctgtca tcgaagaaag cctgagcccc gcgcagatcg acgtgctgct
     1441 cttcgccgca cgtcatccgg aagtgatgtc gattatcgaa agcgtgcgcg gcttcgcaca
     1501 ggggttcaca ccgggcaaac gcggaaaagc gtttcttcaa gccgccgaag atcccgccac
     1561 ctacgggaaa aaacgctgag caaaatgtag ccgtttccgg ctgctccaac gaccacgacg
     1621 tttctcaaaa cgctgaggcc gcaccgctgc cgcgagaacg cggccgaccg
//
LOCUS       DASPLT010001385         1616 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_220960, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001385 DASPLT010000000
VERSION     DASPLT010001385.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1616)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1616)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1616
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_220960"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1520)
                     /locus_tag="VEA63_14100"
     CDS             complement(<1..1520)
                     /locus_tag="VEA63_14100"
                     /inference="COORDINATES: protein motif:HMM:NF024353.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter-associated beta strand
                     repeat-containing protein"
                     /protein_id="HYD85192.1"
                     /translation="MITKSISTTATLKGRRIVALRLAAWIGFLLMPFLTEAQTTLYWD
                     GNGTTGGTGGTNTWTTSTSTANRRWRADSATGTLGSWSNSSPTDYAVFGGTAGTVSWA
                     SNISVRRITFTVSGYTLARTSNTLTLVTDGFIDTGSFNATISGPMAGTVGMTKLGSGT
                     LTLSGASTYTGVTSINAGVLAVSSLANGGNNSNIGKSTSDAANLVLNGGTLRYTNTNA
                     ASTTNRLFTLGAGGGTLDASATGALTFSNTGAIAYTGTGNRTLTLTGSSTAGNTLAAA
                     IANPAGNTTSIVKSSGGTWVVSGNNTYSGTTTIEQGTFAFGSNTAAGTSVITVNGGTL
                     SASGGERTIANNVVVGGNFTIGGTDNLKFEGSINLGGGTRTITVNNTGLTTFAGSITQ
                     PYYSGLVKAGTGTLVLSGDNSYAAPTSINAGTLVLASNTALGASGIYGHTIASGATLA
                     LQGDVSVNQAGFNLSGSGVGGNGAIRNLSGNNTLGGSLILGGATTITSDSGTLTLTG"
BASE COUNT          270 a          447 c          571 g          328 t
ORIGIN      
        1 ccggtgagcg tgagcgtgcc gctgtcggaa gtgatggtcg tggcaccgcc gagaatgagc
       61 gagccgccga gcgtattgtt gccggagaga ttgcgaatcg caccgttgcc accgacgccg
      121 ctgccggaaa gattgaaacc cgcttgattg acgctgacgt cgccttgaag cgcgagcgtg
      181 gcgccgctgg cgatggtgtg gccatagatc cccgacgcgc cgagagcggt gttgctcgcg
      241 agaacgagcg tgccggcgtt gatggaagtc ggcgcggcgt aactgttgtc gccggacaaa
      301 acgagggtgc cggtgccggc tttgaccaag ccgctgtagt agggttgcgt gatggagccg
      361 gcgaaggtgg tgagcccggt gttattcacc gtgatcgtgc gcgtgccgcc gccgagattg
      421 atcgagcctt cgaacttgag attgtcggtg ccgccgatgg tgaagttgcc ccctacgacg
      481 acgttgttgg cgatcgtgcg ctcgccgccg ctggcggaga gggtgcctcc gtttacggtg
      541 atgacggagg tgccggcggc cgtgttgctg ccgaaggcga aggtgccttg ctcgatggtc
      601 gtcgtgccgc tgtaggtgtt gttgccggag acaacccagg tgccgccgct ggacttgacg
      661 atcgaagtgg tgttgccggc gggattcgcg atggcggcag cgagggtgtt gccggcagtg
      721 ctggagccgg tgagcgtgag cgtgcggttg ccggtgcccg tgtaagcgat tgcgccggtg
      781 ttcgagaatg tcagcgctcc ggtggcagag gcgtcgagcg ttccgccgcc ggcacctaac
      841 gtgaacagcc ggttggtggt gctggccgca ttcgtgttcg tgtagcgaag cgtgccaccg
      901 ttgaggacga gattggcggc gtcgctggtg gacttgccga tgttgctgtt gttgccgcca
      961 ttggcgagcg agctcacggc gagcactccg gcgttgatgg aggtcacgcc ggtataggtg
     1021 ctggctccgc tcaatgtgag cgtgccactg ccgagttttg tcatcccgac ggtgccggcc
     1081 atcggtccgc tgatggtcgc gttgaacgaa ccggtgtcga taaatccgtc cgtgaccaac
     1141 gtcagcgtgt tgctggtccg cgccaacgtg tagccgctca cggtgaaagt aatacgacgc
     1201 accgaaatat tcgacgccca cgaaaccgtt cccgctgtcc caccgaatac cgcgtaatcg
     1261 gtcgggctgg agttagacca acttcctagc gttcccgtcg cagaatcggc gcgccaacgg
     1321 cggttcgcgg tggacgtgga cgtcgtccat gtgttggtgc cacccgtgcc gcccgttgtg
     1381 ccattcccgt cccagtaaag tgtcgtctgc gcctcggtga ggaacggcat cagaagaaat
     1441 ccgatccacg ccgcgagccg gagcgcgaca atccgccgac ccttgagcgt cgcggttgtg
     1501 gagatcgatt tggttatcat gagctaaggc ggaacacacc gaaacagcgc gttagccgtg
     1561 cgtcaatgct ttactagggg ataacaccta gggttgtgcg tcttggaaag gaatcg
//
LOCUS       DASPLT010001386         1476 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_140262, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001386 DASPLT010000000
VERSION     DASPLT010001386.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1476)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1476)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1476
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_140262"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(400..1161)
                     /locus_tag="VEA63_14105"
     CDS             complement(400..1161)
                     /locus_tag="VEA63_14105"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HYD85193.1"
                     /translation="MNPVIEPQRKTEFERAWSARFREFAHVHDDDAGIAGWTRTGLAA
                     RLRHFERHFDASRAQGLWLDAGCGAGTYSRHLRRLGADVVGLDYSLESLRKARRRDET
                     DALYCAGDVTRLPLRDGAFDGVLCFGVTQALSGSEAAVRALTAAARANGEVWIDGLNV
                     WCLPHLLEMLSRRLRGRREHLRYETPARVKRALRDAGWRVERYWLPILPARFARFQGW
                     LERGWVVALLHRVPPLGALLSHSFVIRARRGSAKT"
     gene            1197..>1476
                     /locus_tag="VEA63_14110"
     CDS             1197..>1476
                     /locus_tag="VEA63_14110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD85194.1"
                     /translation="MEKLYDAMRNELKALWNKVNDLQGEVAALQPERSSAPAADKVQA
                     IEQGILWDGDSGATRDHWNAALKLRWKKKGGDWRDAKGVPHGRYAYANF"
BASE COUNT          300 a          483 c          466 g          227 t
ORIGIN      
        1 taaggtctct ctggatgcgc cggcgcgaat gaattgcgaa agcggcccgt gactttgccc
       61 tcgctcgtac agctttcgcg cacgcgcgac catcctcggc acttgatcag gctttcccaa
      121 cctctccgat gcctgcatca gcaagccgag tgccttgccg cgcgcagggc gcaaattctt
      181 gatcctgttc gggagcgggc aatgtgcagc aaaagggatg gggcgaatgt aaaaaactgc
      241 gactctgcgc acggcggacc tgccaagcac gacacttgca ctaaggaggc tccgctcaag
      301 gccccttgga tgcgccgtcg cgaaggcggc tcgtaagcga ttgatcgttt gtcctcgctc
      361 gtacagcttc gcccactcgc gaccatcctc ggcgcttgat catgttttcg ccgagccgcg
      421 tcgcgcgcga atcacaaaag agtgactgag caacgcaccg aggggcggca cgcggtgcaa
      481 cagcgcgacc acccagccgc gttcgagcca gccttggaaa cgggcgaaac gcgccggaag
      541 aataggaagc cagtaacgct cgacgcgcca gccggcgtcg cgcaacgcgc gtttcacgcg
      601 ggccggcgtt tcgtagcgca aatgctcgcg acgtccgcgc agccgtcgcg acagcatctc
      661 gagcaagtga gggaggcacc agacattcag accgtcgatc cagacttcgc cgttcgcacg
      721 tgccgcggcg gtgagcgcgc gtaccgccgc ttcggaaccg gacagcgcct gcgtcacgcc
      781 gaaacaaagc acgccatcga acgcgccgtc gcgcaacggc agccgcgtca cgtcccccgc
      841 gcaataaagc gcatcggttt cgtcgcggcg gcgcgccttg cgcagacttt caagagaata
      901 gtcgaggccg acgacgtccg cgcccagccg ccgcaaatga cgggagtacg tgcccgcgcc
      961 gcagcccgcg tcgagccaca gcccttgcgc gcgcgaggca tcgaaatgac gctcgaaatg
     1021 gcgcaaccgc gccgccagtc cggtgcgcgt ccagcccgcg atgccggcgt cgtcatcgtg
     1081 cacatgcgcg aactcgcgaa agcgcgcgct ccaggcgcgc tcgaattcgg tcttgcgttg
     1141 cggttcgatg acgggattca aagacgtgtg ctccgttccg gccccggatg ataaccatgg
     1201 agaaacttta cgacgcgatg cgcaacgagt tgaaagcgct gtggaacaaa gttaacgatt
     1261 tacagggaga ggtggccgca ttgcagcccg aacgatcctc ggcgcccgcc gccgacaagg
     1321 tgcaagccat tgagcagggc attctgtggg acggcgactc cggcgcgacg cgcgaccact
     1381 ggaacgccgc actgaagctg cgctggaaga agaaaggcgg cgattggcgt gacgcaaagg
     1441 gagtgccgca cgggcgctac gcctatgcga acttca
//
LOCUS       DASPLT010001387         1189 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_77167, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001387 DASPLT010000000
VERSION     DASPLT010001387.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1189)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1189)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1189
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_77167"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            60..734
                     /gene="trmD"
                     /locus_tag="VEA63_14115"
     CDS             60..734
                     /gene="trmD"
                     /locus_tag="VEA63_14115"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HYD85195.1"
                     /translation="MPLKIDLLTLFPRMLDSILSESILGKGIAAGLLTVNVHDLRAWT
                     TDKHRTADDRPFGGGAGMVMKPEPVFAAIEQLQTPGCRRIYLTPDGTPLSPAIAHELS
                     QQTHLVLLSGHYEGIDQRIRDSVIDQEISIGDYVLTNGTLAAAVVIDALARFIPGVLG
                     EEKSLTHESFTNKLLDFPQYTRPAEFRGLSVPEVLLSGNHAEIEKWRHARQLEKTRQV
                     RPDLLT"
     gene            741..1154
                     /gene="rplS"
                     /locus_tag="VEA63_14120"
     CDS             741..1154
                     /gene="rplS"
                     /locus_tag="VEA63_14120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007360294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HYD85196.1"
                     /translation="MNPIINEITAAQVNKDITPFKVGDGVRVHTKVREGEKERVQVYA
                     GIVIARKGHGIHETFTVRRISYGEGVERVFPVNSPNIVKVEVEKESEAMKARLYYLRN
                     RTGKAAVAVQVKDRSAEIHAAKVAAAAATAAPSSN"
BASE COUNT          245 a          342 c          360 g          242 t
ORIGIN      
        1 ttgttttggc cggttttgtc tgtgcggctc gcgcttcatt gatcagtttc cattcgtcca
       61 tgccgctgaa gatcgatctg ctcacgcttt tccctcggat gcttgatagc atcctgagcg
      121 agagcattct gggcaaaggg attgcagcgg gattgttgac ggtgaacgtg cacgatttgc
      181 gcgcgtggac gaccgacaag catcgcaccg cggatgaccg gccgttcggc ggcggcgccg
      241 ggatggtgat gaaacccgag ccggtgtttg cggcgatcga gcagttgcag acgccgggat
      301 gtcgtcgaat ttacctcacg ccggacggca ctccgctctc gcctgcgatc gcgcacgagc
      361 tatcgcagca aacgcatctc gtgttgttga gcggacatta tgaaggaatc gaccaacgga
      421 ttcgcgattc ggtgatcgat caggagatca gtattggcga ttacgtgctc acgaacggca
      481 cgctcgcggc cgcggtggtg atcgatgcgc tcgcgcgttt cattcccggc gtgctcggcg
      541 aggaaaagtc attgacgcac gaatccttca ccaacaagtt gctcgacttt cctcaataca
      601 cgcgtcccgc ggagtttcga ggcttgtccg ttccggaggt tctgctttcc ggaaaccatg
      661 ccgagatcga aaagtggcgg catgcgcgac aacttgagaa aacccgtcag gtccgtcccg
      721 atttactcac ataaacagcc atgaacccga ttattaacga aatcaccgca gcccaggtga
      781 ataaagacat cacgccgttc aaagtcggcg acggcgtgcg cgtgcacacg aaggtgcgcg
      841 agggcgagaa agagcgcgtg caggtttacg ccggcatcgt gatcgcgcgg aagggtcatg
      901 gcattcacga gacgttcacc gtccgccgca tcagctacgg cgaaggcgtg gagcgcgtgt
      961 tcccggtcaa ctctccgaac atcgtcaagg tcgaggtcga gaaggaatcc gaagccatga
     1021 aggcgcgtct ttactacctc cgcaaccgca ccggcaaggc cgcggtcgcc gtgcaggtca
     1081 aggatcgttc ggccgagatc cacgcggcga aagtcgccgc tgcggctgct acggccgctc
     1141 cttcgtcgaa ctgatcgacg gcgagtttcc atcctttcga aaagcgagt
//
LOCUS       DASPLT010001388         1990 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_261937, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001388 DASPLT010000000
VERSION     DASPLT010001388.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1990)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1990)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1990
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_261937"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..500)
                     /locus_tag="VEA63_14125"
     CDS             complement(<1..500)
                     /locus_tag="VEA63_14125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD85197.1"
                     /translation="MDNTLTPRPKKSSASTIVIGLLALGVVILAFLLWSRTRAANVRV
                     TDTQTQLEQAKAETADVRTQLADAQAQSANLQKQLDEAKANATQLQTQLDESKAGTAA
                     LQRQVEESQAATQDLQRQLDEAKAGSAELQARADEVQSNASRLQGELEQAQAGTGDLQ
                     KAAEDAR"
     gene            complement(618..1196)
                     /locus_tag="VEA63_14130"
     CDS             complement(618..1196)
                     /locus_tag="VEA63_14130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD85198.1"
                     /translation="MSDPYQTPRKIEPIPAIVIGVLAAALLVVVLIFVNKLQNSQERL
                     SESASELQQARAEIQQLQTELNEARSASGLFQNQVNDAKLAATKTEAQLAEARAESAR
                     LQKQLNDVTTSVGRLQNDLRAAETRSNDIRAQADEAETERAKLNRQLGDLKIEAAQAR
                     RDADETKARLADAQAKLAAAQTQLTENQSKKK"
     gene            complement(1441..>1990)
                     /locus_tag="VEA63_14135"
     CDS             complement(1441..>1990)
                     /locus_tag="VEA63_14135"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HYD85199.1"
                     /translation="GTGLGLPTVRSIVENQRGVITLHSRVGHGTTFQVILPASPDAEF
                     TQGTAPGPAAPRGTGELILVVDDDENVRDVTTAMLSRNGYRVLAAANGTEAVALFAPR
                     SLEISVVVTDLNMPELDGFALAKVVRSLNPSTRILAMSGMSDTSDLRRQNIPGPLLTK
                     PFTSEALLVKIRELLTPSVDMR"
BASE COUNT          299 a          506 c          754 g          431 t
ORIGIN      
        1 cgagcgtcct cggcggcttt ctgaagatcg ccggtgccag cttgggcctg ctcaagttca
       61 ccttggagtc ggctggcgtt gctctggact tcgtcggcgc gagcttggag ttcagcggag
      121 ccggctttgg cttcgtcgag ctggcgttgc agatcctgcg tcgcggcctg cgattcttcg
      181 acctggcgtt ggagtgcagc ggtgcccgcc ttggattcgt cgagctgggt ctggagttgc
      241 gtggcgttgg ccttcgcttc gtcgagctgc ttttgcaggt tcgccgattg cgcttgggcg
      301 tcagcgagtt gggtccgcac gtcggcggtc tcggcttttg cttgctcgag ctgggtttgg
      361 gtatcggtta cgcggacgtt ggcggcgcgg gtgcggctcc agaggaggaa ggcgaggatg
      421 accacgccga gggcgaggag gccgatgacg atggtagaag cggacgactt tttaggacgg
      481 ggtgtaaggg tattatccat ggtgcgataa cctacacgcg aagggccggg aaggatatgg
      541 ggcggaaccc ggcggtgtgg taagtataag ccgcaggcag aggttccgcg atgaaggtgc
      601 aattttgagc gtggcagcta ctttttcttg gattggttct ccgtgagctg ggtctgggcg
      661 gcggcgagct tggcttgggc atcggcaagc cgggctttgg tttcatcggc gtcacggcgg
      721 gcttgggcgg cctcgatttt caggtcgccg agctggcggt tgagtttggc gcgctcggtc
      781 tcggcttcgt cggcttgggc acgaatgtcg ttggagcgag tttcggcggc gcgaagatcg
      841 ttttgcaggc gaccgaccga ggtggtgacg tcgttgagtt gtttttgcag gcgcgcggat
      901 tcggcccgcg cttctgcgag ctgcgcttcg gtcttcgtgg cggcgagttt ggcgtcgttg
      961 acctgatttt gaaacaggcc ggaggcggag cgcgcttcgt tgagttcggt ttgtagttgc
     1021 tgaatttcgg cgcgggcttg ctggagctcg gacgcggact cggagaggcg ctcttgagaa
     1081 ttttggagct tgttgacgaa gatcagcacg accaccagga gggcggcggc gagaactcca
     1141 atgacgatgg cggggatcgg ttctattttg cggggcgttt gataggggtc gctcataacg
     1201 cgcagaagct acaccttgcg cgggcgcggc gaaatagggt gatgccttca gccggtcgcc
     1261 gatcggctgg cgttcgccta tgaaggggga aagcctcaag caaacgacgc gtcgcttttg
     1321 ccaagacgtg cgcaacacgt ggcgcggcga gcgcaccacc tacctgaatt ccgctcgcgg
     1381 gcacaaggct ggcgctacct cagcggccag gactttactt gagccgctcg aggacggcct
     1441 ctacctcatg tcgactgacg gcgtgaggag ttcgcggatt ttaacgagca gggcttcgga
     1501 ggtgaacggc ttcgtgagaa gtggaccggg gatgttttgc cggcgaaggt cgctggtgtc
     1561 gctcatgccg ctcatcgcga gaatgcgcgt cgacgggttc aggctgcgca cgactttggc
     1621 gagcgcgaag ccatcgagtt ccggcatgtt caaatcggtg acgacgacgg aaatttcgag
     1681 gctgcgcggc gcgaagagcg ccaccgcttc ggtgccgttg gcggcggcga gcacgcggta
     1741 gccgttgcgg gagagcatcg cggtggtgac gtcgcggaca ttttcgtcgt catcgacgac
     1801 gaggatgagt tcgcctgtgc cgcgtggcgc ggcggggccg ggagcggtgc cttgggtgaa
     1861 ctcggcgtcg ggactggcgg gaaggatcac ctgaaacgtg gtgccgtggc cgacgcggct
     1921 gtggagcgtg atgacgccgc gctggttttc gacgatgctg cgaacggtgg gcaagccgag
     1981 cccggtgccg
//
LOCUS       DASPLT010001389         1111 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_181085, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001389 DASPLT010000000
VERSION     DASPLT010001389.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1111)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1111)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1111
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_181085"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1111
                     /locus_tag="VEA63_14140"
     CDS             <1..>1111
                     /locus_tag="VEA63_14140"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HYD85200.1"
                     /translation="YAVCGLFAAGSLFGAFRWKVRLIQEQERRLRAQNDLLEAKVKAR
                     TDELANSVSVLQATLDSTADGVVALHFSGEIVSYNTRFTTMWNLPPEAMASDAALRDA
                     SARRTRDPKAFLARIDEIHTQEGEHFDVIELADGRVIERYCKPQSVDGRSVGIVMNFR
                     DITERRRAEEALLASEGFLNSLVENLPVHIFRKDRAGRITFANRRYCERYGRCAADLL
                     GKTAFDLRPHAEAERTEQDDQVVMSTGRPYEGVEIQTLPSGERRYIQIIKVPVLDSAG
                     QCTGVQGMFLDVTEAKLAEARLAEASSLLEAMLDNTPDFIYFKDRESRFVRVSKALLA
                     RVPDPSQVHRLTDFDLFTAEHAQKAFNDEQEIMRTG"
BASE COUNT          211 a          404 c          304 g          192 t
ORIGIN      
        1 ctacgcggtc tgcggcctct tcgccgcggg ctcgttgttc ggcgcgtttc gctggaaggt
       61 gcggctcatc caagagcagg aacgtcgcct ccgcgctcaa aacgacctgc tcgaagccaa
      121 ggtcaaggcc cgcaccgacg agctcgccaa ctccgtctcc gtgctgcaag ccacgctcga
      181 ctccaccgcc gacggtgtcg tcgcgcttca tttctccggc gagatcgtca gctacaacac
      241 ccgcttcacc acgatgtgga atcttccgcc ggaagcgatg gcgagcgacg ccgccttgcg
      301 cgacgcatcg gcccgccgca cgcgcgatcc caaagcgttt ctcgcgcgta tcgacgaaat
      361 ccatacgcag gaaggcgaac acttcgacgt catcgagctc gccgacggcc gcgtcatcga
      421 acgttattgc aagccccaga gcgtcgacgg ccgcagcgtc ggcatcgtga tgaattttcg
      481 cgacatcacc gagcgccgcc gcgccgagga agccctgctc gcgtccgaag gttttctcaa
      541 ctcgctcgtc gaaaacctgc ccgtgcatat tttccgcaag gatcgcgccg gccgcatcac
      601 cttcgccaac cgccgctatt gcgaacgtta tggccgctgc gccgccgacc ttctcggaaa
      661 aaccgccttc gaccttcgcc cgcacgccga ggccgaacgc accgagcaag acgaccaggt
      721 tgtcatgtcc accggccggc cctacgaggg ggtcgagata cagacgctgc cctcgggcga
      781 acgtcgctac atccagatca tcaaggtccc cgtcctcgat tccgccgggc agtgcaccgg
      841 cgtccaggga atgtttctcg acgtcaccga ggcgaagctt gccgaagccc gcctcgccga
      901 agccagcagc ctcctcgagg cgatgctcga taacactcct gacttcattt atttcaagga
      961 tcgcgaatcg cgcttcgtcc gcgtcagcaa agcccttctc gcccgcgtgc ccgatccgtc
     1021 gcaagtccac cgcttgaccg acttcgacct gttcactgcc gagcacgcgc agaaagcgtt
     1081 caacgacgag caggaaatca tgcgcaccgg c
//
LOCUS       DASPLT010001390         1078 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_40777, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001390 DASPLT010000000
VERSION     DASPLT010001390.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1078)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1078)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1078
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_40777"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..392)
                     /locus_tag="VEA63_14145"
     CDS             complement(<1..392)
                     /locus_tag="VEA63_14145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine dehydrogenase
                     (aminomethyl-transferring)"
                     /protein_id="HYD85201.1"
                     /translation="MASPRDLLSPLDTFSRRHTGENSADTAEMLRLLDYPSLDALVDD
                     SLPASIRRGPLDLPPAASESAALAELRELASQNQVFRNCIGLGYYDTLVPGVIQRNIL
                     ENPGWYTAYTPYQAEISQGRLEALLNFQT"
     gene            663..>1078
                     /locus_tag="VEA63_14150"
     CDS             663..>1078
                     /locus_tag="VEA63_14150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD85202.1"
                     /translation="MILDSLRSFATRLLVVLTIGFTIRAPLSAQVALSAGTTTENFDN
                     LAGGLPAGWSIHTGATVATLGSAATLNPATATWANTSGAWKNVAAADNAGFTGAENDA
                     TQATASDRALGIRQTGGFGDPGAAAGFNFSTSGLQVT"
BASE COUNT          187 a          314 c          388 g          189 t
ORIGIN      
        1 gtttgaaaat tcagcagcgc ttcgaggcgg ccttgggaaa tctcggcctg gtaaggcgtg
       61 taagcggtat accagccggg attttcgagg atgttgcgct gaatgacgcc gggaacgagc
      121 gtgtcgtagt agccgaggcc gatgcagttg cggaaaacct gattctggct ggcgagttcg
      181 cggagttcgg cgagcgccgc gctctcgctc gcggcgggcg ggagatcgag cgggccgcgg
      241 cgaatcgagg cgggaagcga gtcgtcgacc agtgcgtcga gcgacggata atcgagcagc
      301 cgcaacatct cggcggtgtc cgcggagttt tcgccggtgt ggcggcggga aaaagtatcg
      361 agcggagaca ggaggtcgcg tggcgatgcc atgggaaaag cttgcaccgt aagagccgcg
      421 tccggccgtg caatggcgat ctcgcccgaa tgagcggcga ccggtgtttt ccgatcgcat
      481 gcatccaagg cgcgatgccg gaagcgggtg tggcgccgtc gtcggcgtgg ttggcaaggg
      541 ccgccagacg catgaaaagc cgcatgatcg cggttgtgcc ggagaacgtt gcaggattgt
      601 tgcggcacgg ggattgcttc cccggcgaac tctttcgagc ctccgcccag ccccttcttt
      661 ccatgatcct cgactccctg cgttcgttcg cgacgcgact gttggttgtt ctcacgatcg
      721 gtttcacgat tcgcgcgccg ctctcggcgc aggtggccct ctccgcgggc accacgacgg
      781 aaaatttcga caacctcgcg ggcggattac ccgccggctg gtcgatccac accggtgcga
      841 ccgtcgccac cctcggtagc gcggcgacgt tgaacccggc gacggcgacc tgggcgaata
      901 cgtcgggcgc gtggaaaaac gtcgcggcag cggacaacgc cggattcacc ggcgcggaga
      961 acgatgcgac gcaggcgacg gcgagcgacc gcgcactggg tattcgtcag acgggcggat
     1021 tcggcgatcc gggcgcggcc gcgggtttca atttttcgac cagcgggctg caggtgac
//
LOCUS       DASPLT010001391         2252 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_262000, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001391 DASPLT010000000
VERSION     DASPLT010001391.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 2252)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2252)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2252
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_262000"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..428)
                     /locus_tag="VEA63_14155"
     CDS             complement(<1..428)
                     /locus_tag="VEA63_14155"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019847.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HYD85203.1"
                     /translation="MSFDVLAPHYTWLERLLAGPRLQRARVAHLDALADCKHLLIAGV
                     GHGHFLRTAAVRFPHLEILSVDASARMLAHAQLHTRSVPNPGRLQFEQATFPRFQPPL
                     ASFDAIATPFFLDCFPPDELATVIASLATAARPRARWLVS"
     gene            complement(447..671)
                     /locus_tag="VEA63_14160"
     CDS             complement(447..671)
                     /locus_tag="VEA63_14160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD85204.1"
                     /translation="MCRKTAAANAILDGPNPPNIKLEPIRTFEGRGVAYLKYGVAKTT
                     HRRNASHATYPGEPTHPVVFHSGGFASIRG"
     gene            complement(760..1326)
                     /locus_tag="VEA63_14165"
     CDS             complement(760..1326)
                     /locus_tag="VEA63_14165"
                     /inference="COORDINATES: protein motif:HMM:NF013989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HYD85205.1"
                     /translation="MPSPKTQFYGAASLDGFLSTTDDNIDWLLQFGDSPPTYANFIAH
                     VGALAMGAATYEWVLANHVKPNSPTPQPWPYEQPTWVFTHRQLPEIPGADIRFVSGDV
                     RPVHAAMTQHARGKNIWLVGGGELVGQFWDHDLLDELLVQIAPVKLGAGKPLLPRAIC
                     TPPLKLVSIEQFGPFAELRYEVPKARPE"
     gene            complement(1497..1901)
                     /locus_tag="VEA63_14170"
     CDS             complement(1497..1901)
                     /locus_tag="VEA63_14170"
                     /inference="COORDINATES: protein motif:HMM:NF016701.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HYD85206.1"
                     /translation="MKPITGSCLCGKVSFEVVGTPLKFLYCHCRSCQKSSGSIHAANL
                     AFPAGSVRWTHGEDLVELFVETKENPGFPRCFCRCCGSPVPKLSRNRQFWVVPSGSLD
                     ADPGMRPQANIYWSEHAPWYVPAGQLAKNDAS"
     gene            complement(2024..>2252)
                     /locus_tag="VEA63_14175"
     CDS             complement(2024..>2252)
                     /locus_tag="VEA63_14175"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HYD85207.1"
                     /translation="YLQRWQPPALMIWGRHDPFFELAETLSWMQDLPRMETHIMDGGH
                     MLLETHATAAASLMLNFITRGRSPVDKGPSA"
BASE COUNT          426 a          578 c          823 g          425 t
ORIGIN      
        1 tcggagacga gccaccgggc tcgagggcgg gcggccgtgg cgagcgaggc gatcacggtc
       61 gcgagctcat cgggtggaaa gcagtcgaga aagaacggtg tcgcgatcgc gtcgaaggag
      121 gcgagtggcg gttgaaagcg ggggaacgtg gcttgttcga actgcagacg gccgggattc
      181 ggaacggagc gggtgtggag ttgagcgtgg gcgagcatgc gggcgctcgc gtcgacgctg
      241 aggatttcga gatggggaaa gcggacggcg gcggtgcgga ggaagtgacc gtggcccacg
      301 cccgcgatga gcagatgttt gcagtcggcg agtgcgtcga ggtgagcgac gcgcgcgcgt
      361 tggaggcgcg ggccggcgag gaggcgttcc agccaggtgt aatgcggcgc gaggacgtcg
      421 aaggacatgg gcgaccgatt attcaattaa ccacgaatgg acgcgaatcc gcccgaatgg
      481 aaaacgacgg gatgtgtcgg ctcgcctgga tatgtggcgt ggctggcgtt gcgtcggtgt
      541 gtcgttttgg cgacgccgta tttcaggtag gcgacgccgc ggccttcgaa ggtgcggatc
      601 ggttcgagtt tgatgttggg cggattggga ccatcaagga tggcgttggc cgctgccgtt
      661 tttcgacaca cgttcaggat aaaccacgga gacacggcgg cacggagaaa cccgagtatt
      721 ctctgtgttc tcggtgcctc tgtggttcac aacagtcggc tattcggggc gggctttcgg
      781 cacctcgtat ctgagttccg cgaaggggcc gaactgctcg atcgagacca acttcaaagg
      841 gggcgtgcag atggcacgcg gcaagagcgg tttgccggcg ccaagtttga cgggggcgat
      901 ctgaacgagg agctcgtcga gcaaatcgtg atcccagaat tgtccgacca actctccgcc
      961 gccgacgagc cagatatttt tgccgcgagc atgttgagtc atcgcggcgt gcacggggcg
     1021 aacgtcgccg ctcacgaaac gaatatcggc gccgggaatc tcgggcagtt gtcgatgggt
     1081 gaagacccac gtcggctgtt catagggcca gggctgaggc gtcggcgagt tgggtttaac
     1141 gtggttggcg agaacccact cgtaggtggc ggcgcccatc gcgagcgcgc cgacgtgtgc
     1201 gatgaagttg gcgtaggttg gtggagaatc gccaaactgg aggagccaat cgatgttgtc
     1261 gtcggtggtg gagagaaagc cgtcgagact ggcggcgccg tagaattggg ttttgggcga
     1321 aggcatggca gggcgtgcga gtggtcggga tggaaccacg aagacacaga ggcacggagg
     1381 cgtttcgatc aagcgaggcg atcgttcgtg ttgcggtagt cgatatcgcg gcagcgagtg
     1441 cgattcgcgg gacgctgcga gcgtggcgtc gccatcgcga gccaacctgc ggcccttcag
     1501 ctggcgtcgt tcttggcgag ttgtcctgcc ggcacatacc aaggagcgtg ttccgaccaa
     1561 tagatgttcg cctgcggcct catgcccgga tccgcatcca atgaaccgct gggcacgacc
     1621 cagaattgcc gattgcgact cagcttgggc accggcgaac cacaacacct gcaaaaacaa
     1681 cgcgggaatc cggggttctc ttttgtctcc acgaagagtt cgacgaggtc ttcgccgtgg
     1741 gtccatcgga cagaacctgc ggggaaggcg aggttcgcgg cgtggatgga gcccgaggac
     1801 ttctggcatg aacggcagtg gcaatagaga aacttgagcg gcgtgccaac gacttcgaag
     1861 ctgacttttc cgcataaaca ggacccggtg attggcttca tagcgatttg tcgaacgtca
     1921 tgctgagccc cggtgcgaca acgtcgaagt agcgctggtg ttcatcgttg aagccggcgc
     1981 ggatcgcgtt gcttgcgggg tcgcgtttgt ggggagcttg gcgtcatgcg ctcggccctt
     2041 tgtctaccgg cgatcgcccg cgcgtgatga agttgagcat gagcgaggcg gccgcggtgg
     2101 cgtgcgtttc aagcagcata tggccgccgt ccatgatgtg ggtttccatc ctcggcaggt
     2161 cctgcatcca cgacagcgtt tcggcgagtt cgaagaaggg atcgtgccga ccccagatca
     2221 tgagcgcggg cggttgccag cgctgcagat ac
//
LOCUS       DASPLT010001392         1246 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_140328, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001392 DASPLT010000000
VERSION     DASPLT010001392.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1246)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1246)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1246
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_140328"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            171..935
                     /locus_tag="VEA63_14180"
     CDS             171..935
                     /locus_tag="VEA63_14180"
                     /inference="COORDINATES: protein motif:HMM:NF021363.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2062 domain-containing protein"
                     /protein_id="HYD85208.1"
                     /translation="MNAAAVPTAQAPQCYVRATPCLSSATHDLRLLTHIRHVSRHGAR
                     TPFFRNARAPLEEPKLARFHPRRKSLLVSPSTLSAPAAPARPRSFWHRRIRDPIVAQL
                     TQGITPHKIALTIAVGSAIALFPILGTTTLLCFLVALVLKLNQPIIQLINQACWPIHL
                     PAIYVCVRFGGRLFGTSHIDLNFHHMSHLLWNEPVRFAHEFGTAGLHAIVAWCLIAPF
                     YIAAVYYCALPLTRSITRAKADAIASAPPPADHPIP"
     gene            complement(1021..>1246)
                     /locus_tag="VEA63_14185"
     CDS             complement(1021..>1246)
                     /locus_tag="VEA63_14185"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HYD85209.1"
                     /translation="APVGMIPPHDGSGPLHVGFGIAAEAYQPWCDRLKAGGVKIESET
                     EWERGGRSLYFRDPDGHLVELITPGIWANY"
BASE COUNT          223 a          505 c          289 g          229 t
ORIGIN      
        1 aacagcggaa gccgccaaag ggtccgtctt gggataaccc aacaccaccg gccgcgtgaa
       61 actcgcccac gcgccgccgg aaattcacca caccggcgcg gctcgaacgc gcgcaccggc
      121 gaaattcaaa gaacgtctga acctctaaca cggtctcgcg accgcccctg atgaatgcag
      181 ccgctgtgcc aaccgcacaa gccccgcaat gttacgttcg cgcaacgcct tgcctctcgt
      241 ccgcaacgca cgatctccgc ctcctcacgc acatccgtca cgtctcgcgc cacggcgccc
      301 gcacgccgtt ctttcgcaac gcacgcgcgc cgctcgaaga gccgaagctt gcccgctttc
      361 atccccgtcg aaaatctctg ctcgtgtcgc cctcgacgct ctccgctccc gccgctcccg
      421 cacggccacg ctcgttctgg catcgtcgca tccgcgatcc gatcgtcgcg caactcaccc
      481 aaggcatcac cccgcacaag atcgccctca ccatcgccgt cggcagcgcc atcgcactct
      541 tccccattct cggcacgacc acgctcctct gcttcctggt cgcactcgtg ctgaagctca
      601 atcagccgat catccagctc atcaaccagg cgtgctggcc cattcatctc cccgccatct
      661 acgtctgcgt tcgcttcggc ggacgccttt tcggaacctc ccatatcgat ctcaatttcc
      721 accacatgag ccatctgctg tggaacgaac ctgtccgctt cgcccacgaa tttggcaccg
      781 ccggtctgca cgccatcgtg gcttggtgcc tgatcgcccc cttctacatc gcggccgttt
      841 actattgtgc gctgcccctc acccgcagca tcacgcgcgc gaaagccgac gccatcgcga
      901 gcgccccgcc gcccgccgat catccgattc cctgagccga actcatgcag cagaaggtgg
      961 agcccgacgt cctcgtcggg ctggctgcga gcgaaacgtg atcgcgtccg cttcgcgcct
     1021 tcaataattc gcccaaattc ccggagtgat taattccacg agatgcccgt cgggatcgcg
     1081 gaaatacaaa ctccgcccgc cgcgttccca ctcggtctcg ctttcgatct tcactccgcc
     1141 cgccttcagc ctgtcacacc acggctgata cgcctccgcc gcgatcccaa atcccacgtg
     1201 caacggtccg ctcccatcat gcggcggaat catccccacc ggcgcc
//
LOCUS       DASPLT010001393         1924 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_282194, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001393 DASPLT010000000
VERSION     DASPLT010001393.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1924)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1924)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1924
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_282194"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(272..1270)
                     /locus_tag="VEA63_14190"
     CDS             complement(272..1270)
                     /locus_tag="VEA63_14190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HYD85210.1"
                     /translation="MSDYRPKATSLRPRRFRPAPISMLLDTPLSASSAPRAAYTDEVI
                     RRRINACPKLGSLQSINRALSDLVHSEQSLNSQIAEIIRRDPSLAARLLRMVNSVYFG
                     LSTRINNIEEAVFFLGLRQIRELSMATPVIEELERLQCNSHAILPWKDLWSHSIATAI
                     VTRDILASTSLQIDDDTDYLIGLLHNVGKVVMATAFPDELSKLIMMPARTPAEFCKFE
                     RELIGWDHGQIGAHYLSRHQLSDEIVTAVLYHNTPAAAPQHQIFAAAVQVADHLVRHS
                     GITGGFEPVAHIEHDSWLLLDGWKILYEAEGPETMLARAALANTLNRLPSILQGLL"
     gene            1519..>1924
                     /locus_tag="VEA63_14195"
     CDS             1519..>1924
                     /locus_tag="VEA63_14195"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HYD85211.1"
                     /translation="MNPAKLQSLLQAGIVHHRAGRFAEAETLYRQARVAAPKHFDVLH
                     LSGLVAYQQARMTEAVDLLTRAHQLDRKSHVCEMRLGLALLAAGRAAEGEAHLRKVVH
                     QNPGYNEGWDNLAYCLKAQDKLVEALACHEKAV"
BASE COUNT          348 a          520 c          708 g          348 t
ORIGIN      
        1 cgaggcggcg ggccggccga acttgcgcgc gaacgagagg tttacggcga gcagccggcc
       61 ttcgacgtcg tggcaatacg cgggatcggc gctggtttcc cagggcccga gccaattttc
      121 agtcggcgac gtggtggcga cgccagcagc ggaatcgctt agcacggctg acatgatccc
      181 agtgtatcgg tgcgatggag acgaacttga gcggcggcgg acagtgggcg aaatgggaac
      241 ctcccgccgc tcacgccgca gatcacgggc gtcacagcag gccctgcagt atcgaaggaa
      301 ggcggttgag cgtgttggcg agcgcggcgc gggcgagcat cgtctcgggg ccttcggctt
      361 cgtagagaat tttccagccg tcgagcagca gccacgaatc gtgttcgatg tgagcgacgg
      421 gttcgaagcc gccggtgatg ccggagtgac gcacaagatg atcggcgacc tgcacggcgg
      481 cggcgaagat ctgatgctgc ggcgcggcgg cgggcgtgtt gtgatagaga acggcagtga
      541 cgatttcgtc ggagagttgg tggcggctga ggtaatgcgc gccgatctga ccgtgatccc
      601 agccgatgag ctctcgctcg aatttgcaga attcggcggg cgtgcgggcg ggcatcatga
      661 tgagcttgct gagctcgtcg gggaaggcag tcgccatcac gaccttgccg acgttgtgga
      721 ggagaccgat caggtagtcg gtgtcgtcat cgatctgcag ggaggtcgag gcgaggatgt
      781 cccgcgtgac gattgcggtg gcgatgctgt ggctccacaa gtccttccac gggaggatcg
      841 cgtgggagtt gcactgcaaa cgctcgagtt cctcgatcac cggcgtggcc atcgagagct
      901 cgcgaatctg gcggaggccg agaaaaaaca cggcctcctc gatgttgttg atgcgtgtcg
      961 agagaccgaa gtagacggag ttcaccattc gcagaagtcg cgcggcgagg gaagggtcgc
     1021 ggcggatgat ctcggcgatc tgcgaattca aactttgctc agagtgcacg agatcgctca
     1081 gcgcgcgatt gatgctttgg agcgaaccga gcttcggaca ggcgttgatc cgacgccgga
     1141 taacttcgtc ggtgtaggcg gcgcgaggtg cggaggaggc ggaaagtggt gtgtcgagca
     1201 gcatgctgat gggcgcgggg cgaaagcggc gtggccgcag cgatgtcgcc ttgggtcgat
     1261 aatcggacat cgcccgggat gcttgagccc gattcgattc aagccggcga tcgttcgcgc
     1321 cgatttctga aacacacacg acgtgcagct tgctttgttt cagcgccgct tcctcggggt
     1381 tggcagatcg ttaatcgact ggcgaccggg gcggagcttt ggcggaaggg aaaccggcag
     1441 cgtagggcgg gcttcagccc gctttcttgc gcggggtctt gatggcgggc tgaagcccgc
     1501 cctacgttgg tcgcgcgcat gaaccctgcg aaactccagt cgttgttgca ggccggcatc
     1561 gtgcatcacc gcgcggggcg cttcgcggag gcggagacgc tttaccggca ggcgcgtgtg
     1621 gcggcgccga agcactttga cgtgttgcac ctgtcgggac tcgtcgccta ccagcaagcg
     1681 cgcatgaccg aggcggtcga tctgctcacg cgcgcgcatc agctcgatcg aaaatcgcac
     1741 gtgtgcgaaa tgcggcttgg gttggcgctg ctcgcggcgg gccgcgcggc ggagggcgag
     1801 gcgcacctgc ggaaggtcgt gcatcagaat cccggataca atgaaggctg ggacaatctc
     1861 gcttactgcc tgaaggcgca ggacaagctc gtggaggcgc tggcgtgtca cgagaaggcc
     1921 gtga
//
LOCUS       DASPLT010001394         1317 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_17122, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001394 DASPLT010000000
VERSION     DASPLT010001394.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1317)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1317)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1317
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_17122"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..721)
                     /gene="eno"
                     /locus_tag="VEA63_14200"
     CDS             complement(<1..721)
                     /gene="eno"
                     /locus_tag="VEA63_14200"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000103948.1"
                     /note="catalyzes the formation of phosphoenolpyruvate from
                     2-phospho-D-glycerate in glycolysis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HYD85212.1"
                     /translation="MSTTITAITAREIIDSRGNPTVEVDVKLASGALGRAAVPSGAST
                     GEHEAIELRDGDKLRYGGKGTQKAVGNVKSKIAPALIGFDALDQIGVDRAMLKLDGTK
                     TKSKLGANAILGVSMATAKAAANALGQPLYKYLGGPNAKVLPVPMMNIMNGGAHSDAP
                     IDFQEFMIMPTGAKSFSEALRMGCEVFHSLKKVLKDKGLATAVGDEGGFAPDLRTNAE
                     ALELIVTAIEKAGYRPAEQIYL"
     gene            complement(1056..>1317)
                     /locus_tag="VEA63_14205"
     CDS             complement(1056..>1317)
                     /locus_tag="VEA63_14205"
                     /inference="COORDINATES: protein motif:HMM:NF012253.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HYD85213.1"
                     /translation="ADLLLTRNDNGDTPLHAAAYEGHLDQVPLGLLTEENLNVRNYEG
                     VSVGRTAINCGYSEHIPAPFRPKERGPIGRFLERIGATRPPF"
BASE COUNT          223 a          344 c          488 g          262 t
ORIGIN      
        1 cgaggtagat ctgctcggcg gggcggtagc cggccttctc gatcgcggtg acgatgagct
       61 cgagcgcctc cgcgttcgtg cgcaggtcgg gcgcgaaacc accttcgtcg ccgacggcgg
      121 tggcaaggcc cttgtctttg agaacctttt tcagcgagtg gaaaacttcg cagcccatgc
      181 ggagtgcttc ggagaaggac ttcgcgccgg tgggcatgat catgaattcc tggaaatcga
      241 tcggcgcatc ggagtgcgcg ccgccgttca tgatgttcat catcggaacg gggaggactt
      301 tcgcgttggg gccaccgagg tatttgtaga gaggctggcc gagggcgttg gcggcggctt
      361 tggcggtggc catggagacg ccgaggatgg cgttggcgcc gagcttcgac ttggtcttgg
      421 tgccgtcgag cttgagcatg gcgcggtcga cgccgatctg gtcgagggcg tcgaaaccga
      481 tgagcgcggg agcgatcttg gattttacgt tgccgacggc tttctgggtg cctttgccgc
      541 cgtagcggag tttgtcaccg tcgcgcagtt cgatcgcctc gtgttcgccg gtggaggcgc
      601 cggaaggcac cgcggcgcgg ccgagggcgc cggaggcgag tttgacgtcg acttcgaccg
      661 tgggattgcc gcgggagtcg atgatttcac gggctgtgat cgctgtgatg gtggtgctca
      721 tggatagaat gaaacgctca cgtgtggcgg gttgggtgcg acggggcaag tctggggttg
      781 agccacgcgc caagcgccaa gcgcgaagct ccaaactcca acctccaagc tccagagaag
      841 atccaaaaac caaatctcaa tggtcggcgt tttggaacgt ggtgcttggt gcttcaatgg
      901 agcttggagg ttggtgcttg gagctttccg ggggcacaaa aaaagcccga ccagaggtca
      961 ggcgctacga tggggaggtc gcaagtccaa cttcggacgc ctcggagagg cgtccctacc
     1021 gcgctggcgc gcagcttcgg aagccaaccc gaaacttaaa agggcgggcg ggtggcgcca
     1081 atgcgttcga ggaagcggcc gatggggccg cgttctttcg gacggaacgg cgccgggatg
     1141 tgctcgctgt agccgcaatt gatggcggtg cggccgacgc tgacaccctc gtagttgcgg
     1201 acgttgagat tttcttcggt gagcaggccg agcggcactt ggtcgagatg gccttcgtag
     1261 gcggcggcgt gaagcggggt gtcgccatta tcgttgcgcg tgaggagcag gtcggcg
//
LOCUS       DASPLT010001395         1603 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_77242, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001395 DASPLT010000000
VERSION     DASPLT010001395.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1603)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1603)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1603
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_77242"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1083
                     /locus_tag="VEA63_14210"
     CDS             <1..1083
                     /locus_tag="VEA63_14210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009514032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HYD85214.1"
                     /translation="RAFDHDFMGGKVYGRTFVVEAARFEDLPEERLTALPLGRNLDGC
                     RIGFDLGGSDRKAAAVIDGRLVFAEEIPWNPYFEKDPAYHIEGVHDSLRRMAAHLPRV
                     DAIGGSAAGVYVDNEVRVASLFRGIAPERFEREIRRMFFTLQERWGGVPFEVANDGEV
                     TALAGSMALDDNAVLGVSMGTSQAAGYVTPGGGITPWLNELAFAPIDYHEAAPRDEWS
                     GDSGCGVQYFSQQGVGRLAIAAGFEFPDAQPLPERLIEVQKAMAAGDPRARAVYESIG
                     VVFGYAIGHYAEFYELRNLLVLGRVTSGSGGELLLAQAREVLRCEFPTLAETIRLHTP
                     NEQEKRHGQAVAAASLPSLRGKKTSS"
     gene            1080..>1603
                     /locus_tag="VEA63_14215"
     CDS             1080..>1603
                     /locus_tag="VEA63_14215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009514033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HYD85215.1"
                     /translation="MKLSQLQADLYVPFGDASPEQALARVTHLCVGAHQDDIEIMAHS
                     GISDCLDTPGKAFGGVVVTNGAGSPRTGTYARMTDQEMQGVRREEQRAAARLGRYAIQ
                     LQLAHPSAAVKTPGHPGVVADLTAIFGGCRAEVVYLHNPADKHDTHVAVLLRCLEALR
                     ALPLAQQPQRVLGC"
BASE COUNT          261 a          459 c          594 g          289 t
ORIGIN      
        1 cgggcgttcg atcacgactt catgggcggc aaagtttacg ggcggacgtt cgtcgtcgag
       61 gccgcgcgtt tcgaggattt accggaggag cgtctgactg cgttgccgct cgggcggaat
      121 ctcgacggtt gccgcatcgg cttcgatctc ggcggcagcg atcgcaaggc ggcggcggtg
      181 atcgacggcc ggctcgtgtt tgcggaggag attccgtgga atccgtattt cgaaaaagac
      241 ccggcgtatc acatcgaagg cgtgcacgat tcgctgcggc gcatggcggc gcatctgccg
      301 cgcgtcgatg cgatcggtgg cagtgcggca ggcgtgtatg tggacaacga ggtgcgcgtc
      361 gcgtcgctgt ttcgcgggat cgccccggag cgtttcgagc gggaaatccg gaggatgttt
      421 ttcacgctgc aggagcggtg gggcggcgtg ccgtttgaag tcgcgaacga tggcgaagtc
      481 acggcgctgg ccggttcgat ggcgctcgac gacaacgcgg tgttgggcgt gtcgatgggc
      541 acgagccagg cggccggcta cgtgacgccg ggcggcggaa tcacgccctg gctcaacgag
      601 ctggcgttcg cgccgatcga ttatcatgag gcggcgccgc gcgacgagtg gtccggcgac
      661 agcggttgcg gcgtgcagta tttttcgcag cagggcgtgg gacgtctcgc gatcgcggcc
      721 ggcttcgagt ttccggacgc gcagccattg cccgagcgat tgatcgaggt gcagaaagcg
      781 atggccgcgg gcgatccgcg cgcgcgcgcc gtttatgaat cgatcggcgt cgtgttcggg
      841 tatgcgatcg ggcactacgc ggagttttac gaattgagaa acctgctggt gctcggacgc
      901 gtgacgagcg gatcgggtgg cgaacttctg ctcgcgcaag cgcgcgaggt gttgcgctgc
      961 gaatttccga cgctggcgga aacgatccga ttgcacacgc ccaacgagca ggagaagcgt
     1021 cacgggcagg ccgtcgccgc cgccagcctg ccgtcgctgc gcggaaagaa aacgtcttca
     1081 tgaaactgtc tcaactccag gccgacctct acgttccgtt cggcgatgcg tcgcccgagc
     1141 aggcgctcgc gcgcgtgacg catttgtgcg tgggtgcgca tcaggacgat atcgagatca
     1201 tggcgcattc cgggatctcc gattgtctgg acacaccggg caaggcgttt ggcggcgtgg
     1261 tcgtgacgaa cggcgcgggc tcgccacgaa cgggcacgta tgcgcggatg accgaccaag
     1321 agatgcaagg cgtgcgacgc gaagagcagc gcgccgcggc gcggttggga cgttatgcga
     1381 ttcaactcca gctcgcgcac ccgagcgccg cggtgaaaac accggggcat ccgggcgtgg
     1441 tcgcggattt gacggcgatc ttcggcggtt gccgcgcgga ggtggtgtat ttgcataatc
     1501 cggccgacaa gcacgacacg cacgtcgcgg tgctgttgcg ctgtctcgaa gcgttgcgtg
     1561 cgctgccgct ggcgcagcag ccgcagcggg tgctcggctg cga
//
LOCUS       DASPLT010001396         1088 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_282250, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001396 DASPLT010000000
VERSION     DASPLT010001396.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1088)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1088)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1088
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_282250"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            335..>1088
                     /locus_tag="VEA63_14220"
     CDS             335..>1088
                     /locus_tag="VEA63_14220"
                     /inference="COORDINATES: protein motif:HMM:NF018178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HYD85216.1"
                     /translation="MLACASVNAAEGWRSLWNGQDLTGWTTWLQRPEPTSAVPGLARG
                     ADGKYLEAIGANRDPLGVFTVVNVDGPPALRISGEVFGEIRTVDSFENYRLRLQFKWG
                     TQKRPPRDKPNVRRDSGLLYHVHALPGAEGRTWARSIELQIQEHDTGDLWAVGSEISV
                     RSKKRDGEHPVYVYDPDGEWNVFSQVTGKVGRCVKFPDNENPTGEWNTVELICLGEDA
                     IHLVNGKVVMRLHGPRRIDGTSPQKVTAGQLIL"
BASE COUNT          209 a          268 c          411 g          200 t
ORIGIN      
        1 aaaatcgcga tggcgcagcc gcggctgccg ttgattttga tgaccggcta cgcgtcggcg
       61 ttgaacgagc cgaaagtgaa ggcgatggga ttttccgcga tgctgatgaa gccggtgacg
      121 atggaggaac tggatcgcgc gattcgagca atgcgacgga agtgaaacgc gcgaacgtcc
      181 gcggcgagcc gcggtgagaa agatgtaacc gcgcggggaa gggcgggacg gcgatgaagt
      241 gtgtcatcgc cgggaatcga cattgcgcgg cggagagcga ttgaaacacg ttggcggtct
      301 tatgcgccgc cctgtcttgc tggttctcgg ttctttgttg gcctgcgctt cggtgaacgc
      361 ggcggaggga tggaggtcgc tttggaacgg ccaggatctc acgggatgga cgacgtggtt
      421 gcaacgtccg gagccaacat cggcggtgcc gggattggcg cgcggtgcgg acggaaaata
      481 tctcgaggcg atcggcgcga accgcgatcc gctgggcgtg ttcacggtcg tgaacgtcga
      541 tggtccgccc gcgttgcgga tttcgggcga ggtgtttggc gagattcgca cggtggactc
      601 gttcgagaat taccgcctgc gtttgcagtt caaatggggc acgcagaagc ggccgccgcg
      661 cgacaagccg aacgtgcggc gcgatagcgg gctgttgtat catgtgcatg cgttgccggg
      721 cgcggaaggt cgcacgtggg cgcgctcgat cgagctgcag attcaggaac acgacactgg
      781 tgatctgtgg gcggtgggct cggagatttc ggtgcgctcg aaaaagcgcg acggggagca
      841 tccggtctac gtttacgatc cggacggaga gtggaacgtg ttttcgcagg tgacggggaa
      901 ggtggggcgc tgcgtgaaat ttcccgacaa cgagaatccg acgggcgagt ggaacacggt
      961 cgagttgatc tgcctcggcg aggacgcgat ccacctcgtg aatggaaaag tcgtgatgcg
     1021 gctacacggg ccgcgtcgga tcgacgggac gtcgccgcaa aaggtgacgg cgggacaact
     1081 gattctgc
//
LOCUS       DASPLT010001397         3433 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_221041, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001397 DASPLT010000000
VERSION     DASPLT010001397.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 3433)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3433)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3433
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_221041"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            53..217
                     /locus_tag="VEA63_14225"
     CDS             53..217
                     /locus_tag="VEA63_14225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD85217.1"
                     /translation="MQQPVHAWPRGATAGVLLALIVGIPLFAAILADATTGGSPNPKV
                     AEVEVSDVSP"
     gene            complement(540..1235)
                     /locus_tag="VEA63_14230"
     CDS             complement(540..1235)
                     /locus_tag="VEA63_14230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD85218.1"
                     /translation="MKQRFLIALLTIAVLGAGFFAGVWTERNACKVPKPPALLGELSA
                     PQSNLSPTPAAPKVNTAELAAEIEKLRPEIEKFRLRMLEIDREMDRDIDAILRPDQSE
                     KFQAIVKYYADLRAKEAAAGTLSTPLTPEEITKLQQKPLYKMLSIVVVPMRLEWNTRD
                     LNLDERQREQLRELLKQRRDKFLALVDSSPPPSLTLSQLVPLAQRLVDSSKSTDATGE
                     AEAVAGEPTKKEK"
     gene            complement(1232..1501)
                     /locus_tag="VEA63_14235"
     CDS             complement(1232..1501)
                     /locus_tag="VEA63_14235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD85219.1"
                     /translation="MKPESFAWLALNERAASLLRPGFAERTLRAAREIAPTFASQCML
                     SAATAAVCLVLIFVVHTRMTENETARNLAGWQQIALEAEMLAQLR"
     gene            complement(1491..2039)
                     /locus_tag="VEA63_14240"
     CDS             complement(1491..2039)
                     /locus_tag="VEA63_14240"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HYD85220.1"
                     /translation="MDPSSPTALDELVRLAQKGNEAAQRTLIFSYQRRIAGFVYAITG
                     RSDAVEDLAQLVFIKMIRALSRLHDAAQFEAWLFRLARNTCIDHLRRQKLRRIFTPFA
                     AEHENIPEPPGAVDSEELDALRHALQQLPPKDRALLALAQEGRSQVEMAAATGTSVMA
                     VKARLHRAREKLRQFYQRPHET"
     gene            2158..2811
                     /locus_tag="VEA63_14245"
     CDS             2158..2811
                     /locus_tag="VEA63_14245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD85221.1"
                     /translation="MLNRQLAASARVAILALVLGAAGCSTAHDRDRAAETSLSSRVSL
                     AGEQSFFDDKLFVTATVGPREHRSSDRNGHDEANGRRRRRGGSGEGEARRRRAQDDSD
                     HPAGPRMRVGVPALVLRVTLKNTSDETLVVTIRDVKSDLGNFAARPERVTLEPQQTVE
                     LDPMFSVIDSIGGDILLTIALHSAGKNESHTLTLRSTPSSAPLPRAADATPAANVAD"
     gene            complement(2828..>3433)
                     /locus_tag="VEA63_14250"
     CDS             complement(2828..>3433)
                     /locus_tag="VEA63_14250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HYD85222.1"
                     /translation="RLKKLKTMRDTDAAALSDSIEICQGEVRRLDGIISNFLEAIRPR
                     APDLVETNLAEVLAEVLRFQQRELADRGIVVEAETPDELPMVMADHNQLKQVFFNIVK
                     NAVEAMAPGGELKIRSHADDDNVYLLFGDSGAGIKQEDLVRLFQPYHTTKSTGHGLGL
                     MIVQRIMRDHGGQIGIESKEGVGTLVTLQFPRKDRRVRMLQ"
BASE COUNT          632 a         1155 c          991 g          655 t
ORIGIN      
        1 tgtcgtgctt cgaaacgcaa cctcttgcaa aatccggcgt cccactctcg caatgcaaca
       61 acctgtccac gcgtggccga ggggagcgac ggccggcgtg ctgctcgcac tcatcgtcgg
      121 gattcctttg ttcgcggcca ttttggccga cgcgaccacc ggcggcagcc cgaatccgaa
      181 agtggcggag gttgaagtgt ccgacgtctc gccctagtag ggtgtcccac gaaacgtgaa
      241 taaatcgaag ggcgcggcag cgccggtagg gacgcctctc cgaggcgtcc gcgcaaactc
      301 tgggacaccc tgccagcggc tccgctccgg ccgagaacat atccgaaatg agaatacagt
      361 tcactgctcg tcattggtga tctccgcgaa cggaatgcat tcccaaccag acttccgcgc
      421 gtcgccgacc aggcgaagca cgcactcctc ccccgcgcgc cggcctgcgg aaaaatcagc
      481 gcagattagc ggttcaagaa ctctgacccc cgtggcagac ttcggccgct ctctactcct
      541 tacttctcct ttttcgtcgg ttcgcccgcc acggcttccg cttctccggt cgcgtcggtt
      601 gacttcgacg aatccaccag acgctgcgcg agcggcacga gctgactcaa cgtcaggctc
      661 ggcggcggcg aggaatcgac gagtgcgaga aacttatcgc ggcgttgctt caacagctcg
      721 cgcaactgct cgcgctggcg ctcgtccaag ttcaaatcgc gcgtgttcca ttcgaggcgc
      781 atcggcacca cgacgatcga cagcatctta tagagaggct tctgctgcag cttggtgatc
      841 tcctccggcg tgagcggggt cgagagcgtg ccggcggccg cttctttcgc gcgcagatcg
      901 gcgtagtatt tcacgatcgc ctggaatttt tcgctttgat ccggacgcag gatcgcatcg
      961 atgtcgcggt ccatttcgcg atcgatctcg agcatccgca gacgaaactt ctcgatctcc
     1021 ggacgcagtt tctcgatctc cgcggcgagc tcggcggtgt tgaccttcgg cgcggcgggc
     1081 gtcggagaaa gatttgattg cggcgccgaa agctcgccca gcaacgccgg tggctttggc
     1141 accttgcacg cgttgcgctc cgtccacacg ccggcgaaaa agccggcgcc gagcacggcg
     1201 atcgtgagca aggcgatgag aaaacgttgt ttcatcgaag ctgagccagc atctccgctt
     1261 cgagcgcgat ctgctgccag cccgcgagat tgcgggccgt ttcgttttcg gtcatgcgcg
     1321 tgtgcaccac gaagatcagg acgaggcaaa ccgccgccgt cgccgcgctc agcatgcatt
     1381 ggctggcaaa cgtgggcgcg atttcccgcg cggcacgaag cgtccgctcg gcgaagccgg
     1441 gacgcagcaa agatgcggca cgctcattca gcgcaagcca ggcaaaggat tcaggtttca
     1501 tgtggacgtt gataaaattg acgaagtttc tcccgtgcgc gatgcagccg cgccttgacg
     1561 gccatcacac tcgtgccggt cgccgcggcc atctcgacct ggctgcggcc ttcctgcgcg
     1621 agcgcgagca gcgcgcgatc tttcggcgga agctgctgca acgcgtgccg cagcgcatcc
     1681 aactcctccg aatccaccgc gccgggcggc tccggaatgt tttcgtgctc cgcggcgaac
     1741 ggcgtgaaga ttcgccggag cttctgccga cgcaagtgat cgatgcaggt attgcgcgcg
     1801 agccgaaaca gccacgcctc gaattgcgcc gcatcgtgca accggctcag cgcccggatc
     1861 atcttgatga agaccaattg cgcgagatct tccaccgcat ccgagcggcc cgtgatcgcg
     1921 taaacgaagc ccgcgatgcg acgttgataa ctgaaaatga gcgtccgctg ggcagcctcg
     1981 ttgccctttt gcgccaggcg aacgagctcg tccaaagcgg tcggagaaga tggatccatg
     2041 cttttgcatg cgtctgctcc gcctgacgaa gacgttgttg ggaagttgcg gatttcacct
     2101 taatccccaa gcgcgccgca gcgtgcaaca tttcttcttg gttcaagtca cattgccatg
     2161 ctgaaccgcc agttggcagc ctccgcccgc gtcgcaattc tcgcccttgt gctcggcgcg
     2221 gccggctgca gcacagcgca cgaccgcgat cgcgcggccg aaacttccct ctcgtcacgc
     2281 gtctctctcg ccggcgagca aagcttcttc gacgacaagc tcttcgtcac cgcaaccgtc
     2341 ggtccgcgcg aacaccggag ctccgaccgc aacggccacg acgaggcaaa cggccggcgt
     2401 cgccggcgtg gcggttccgg cgaaggcgaa gcgcgtcgcc gtcgcgctca agatgactcc
     2461 gatcatcccg ccggcccgcg aatgcgcgtc ggcgttcccg ccttggtgtt gcgcgtcaca
     2521 ctgaagaaca cctccgacga gacgctcgtc gtcacaatcc gcgacgtgaa gtccgacctc
     2581 ggcaacttcg ccgcgcgacc ggaacgcgtg acactcgaac cgcaacaaac cgtcgagctc
     2641 gatccgatgt tctccgtgat cgattcgatc ggcggcgata ttctcctcac gatcgccctc
     2701 cactccgccg gaaaaaacga atcgcacacg ctcacgctcc ggtccacgcc gtcatcggcg
     2761 ccactcccgc gcgcagccga cgcgacgcct gcggctaacg tagcggactg atgttccgcg
     2821 cgcgatttta ctgcagcatc cgcacgcggc gatccttgcg cggaaactgc aatgtcacga
     2881 gcgtgccgac gccttccttg ctctcgattc cgatctgccc gccgtgatcg cgcatgatcc
     2941 gctgcacgat catcaatccg agtccgtgcc ccgtcgactt cgtggtgtga tacggttgaa
     3001 acaaccgcac caaatcctcc tgcttgatgc ccgctcccga gtcgccaaac agcaggtaaa
     3061 cattgtcgtc gtcggcgtgc gaacggatct tcagctcgcc accgggcgcc atcgcttcga
     3121 ccgcgttttt gacgatgttg aaaaacacct gcttcagctg attgtgatcc gccatcacca
     3181 tcggcaactc gtccggcgtc tccgcctcga cgacaatgcc acgatccgct agctctcgct
     3241 gctgaaaacg cagcacctcg gcgagcactt cagcgagatt cgtctccacc aaatccggcg
     3301 cgcgcggtcg gatcgcttcg aggaaattgc tgatgatgcc gtcgagccgc cgcacttcgc
     3361 cttggcagat ctcgatcgag tccgagagcg ccgcggcgtc ggtgtcgcgc atcgttttta
     3421 atttcttcaa ccg
//
LOCUS       DASPLT010001398         1083 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_303943, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001398 DASPLT010000000
VERSION     DASPLT010001398.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1083)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1083)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1083
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_303943"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..407
                     /locus_tag="VEA63_14255"
     CDS             <1..407
                     /locus_tag="VEA63_14255"
                     /inference="COORDINATES: protein motif:HMM:NF018178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HYD85223.1"
                     /translation="YFVDGRSSIGLEYQILDDARHPDAKQGRDGNRTLASVYDLYPAS
                     DAKPTVRIGEWNTARIVSRGAHVEHWLNGEKVVEYERFTGGFRRQVQASKYKNTPGFG
                     EWRDGHILLQDHGDAVHFRNLKIRPLSDEASR"
     gene            433..>1083
                     /locus_tag="VEA63_14260"
     CDS             433..>1083
                     /locus_tag="VEA63_14260"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HYD85224.1"
                     /translation="MNRRTFIRQATTAGVGLALAGKFATLARAAGSPSANRRLRIAIA
                     GTNSRGLAHVALFGAVPDVEIAYICDVDDRAIVKGLEATAKVQTTVPKGVRDFRRALE
                     DPSIDALTIATPDHWHAPMAILALAAGKHVFVEKPCSHNPREGELLIAAANKAGRVVQ
                     MGNQRRSFPAMQEAIAQIHEGAIGRAYSARAWYANSRTSIGRGQKTPVPSWLDYDLW"
BASE COUNT          219 a          320 c          357 g          187 t
ORIGIN      
        1 aatacttcgt cgacggcagg tcttcgatcg gcctcgagta tcagattctg gacgacgcgc
       61 gtcatccgga tgccaagcag ggacgcgacg gcaatcgcac gctggcgtcg gtctacgacc
      121 tgtatccggc gtcagacgcc aaaccaacgg tgcggatcgg ggaatggaat acagcgcgga
      181 tcgtaagccg cggagcgcac gtggagcact ggttgaacgg cgaaaaagtc gtcgaatacg
      241 aacggttcac cggcggtttt cgtcgccagg tgcaggcgag caaatacaag aacacgcccg
      301 gttttggcga atggcgggat ggacacatct tgttgcagga tcacggtgac gccgtgcact
      361 ttcgtaatct caagattcgt ccgttgtcgg atgaagcctc acgttaaccc caacccaaaa
      421 actttgtcct ttatgaacag acgcacattt atccgccaag cgacgaccgc cggtgttggc
      481 ctcgcactcg ctggaaagtt cgccacgctt gcgcgcgcgg cgggatcgcc gtccgccaac
      541 cgccgactcc gcatcgcgat tgccggcacg aacagccgcg ggctcgcgca cgtggcgctg
      601 ttcggcgcgg tgccggacgt tgaaatcgcg tatatctgcg acgtcgacga ccgcgcgatc
      661 gtgaaagggc tcgaggcgac ggcgaaggtg caaacgacgg tgccgaaagg cgtgcgcgat
      721 ttccggcgcg cgctggaaga tccgagcatc gacgcgctca cgatcgcgac gccggatcac
      781 tggcatgcgc cgatggcgat ccttgcgctc gcggcgggga agcacgtgtt tgtcgagaag
      841 ccgtgcagtc acaatccgcg cgaaggagaa ttgctgatcg cggcggcaaa caaggccggc
      901 cgcgtcgtgc agatgggtaa tcagcggcga tcgtttcccg cgatgcagga ggcgatcgcg
      961 cagattcacg aaggcgcgat cggccgcgcg tattcggcgc gggcgtggta tgcgaatagc
     1021 cgcacctcga tcggccgcgg gcagaaaacg ccggtgccct cgtggctcga ttacgatctt
     1081 tgg
//
LOCUS       DASPLT010001399         1967 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_77318, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001399 DASPLT010000000
VERSION     DASPLT010001399.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1967)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1967)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1967
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_77318"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1248
                     /locus_tag="VEA63_14265"
     CDS             <1..1248
                     /locus_tag="VEA63_14265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007360008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HYD85225.1"
                     /translation="EMFVGVGASRVRDMFEQGRKSAPCLIFIDEIDAVGRQRGAGLGG
                     GNDEREQTLNSLLVEMDGFDTTEGVIIIAATNRPDVLDSALLRPGRFDRQIYVDLPDL
                     VGREQILRVHARKITLSENVDLAVIARGTPGLSGAELANLLNEAALLAARRNKKKVEM
                     IDVDDAREKVQFGRERRRVMDDEEKKLTAYHEAGHALVQAVLDDGHMPVHKVTIIPRG
                     HSLGSTMFIPKKDILTHAMKRMLNQIAMGLGGRIAEELVMGDVSSGAAGDIKQVTKIA
                     RHMVCDWGMSSLGMIAYGESSDTVFLGREITRSQTYSEETARKIDAEVYRIVDDQYKR
                     AKEIITSRRDVLDKIASALLEHETIEGKHVLEVLQFGEIKSPIVREPVRQPEDKGPGK
                     KTPGKSSAPEGLAPGTAPAPNPA"
     gene            1368..>1967
                     /locus_tag="VEA63_14270"
     CDS             1368..>1967
                     /locus_tag="VEA63_14270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009513581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HYD85226.1"
                     /translation="MKICCIGAGYVGGPTMAMIALKAPDIEVRVVDMNEARIAAWNSE
                     TLPIYEPGLDEVVCEARGRNLFFTTEVRAAIECSDIIFVAVNTPTKTYGVGSGRAADL
                     RFIESVARTIAEVATTPKIIVEKSTIPVKTAETIKDILAANTRGVKFEVLSNPEFLAE
                     GTAIADLQMPDRVLIGGERTPGGEAALQVLADVYARWVPR"
BASE COUNT          422 a          510 c          694 g          341 t
ORIGIN      
        1 gagatgttcg tgggcgtcgg cgcaagccgc gtgcgcgaca tgtttgagca gggccgcaag
       61 agcgcgccgt gcttgatctt catcgatgaa atcgacgcgg tgggtcgtca gcgcggcgcc
      121 ggactcggcg gcggcaacga tgaacgcgag cagacattga actcgctgct cgtcgagatg
      181 gacggcttcg acaccacgga aggcgtcatc atcatcgcgg cgacgaaccg gccggacgtg
      241 ctcgacagcg cgttgctgcg gccgggccgc tttgatcgcc agatttacgt ggacctgccg
      301 gacttggtgg gccgcgagca gatcctgcgc gtgcatgcgc ggaagatcac gttgtcggaa
      361 aacgtcgacc ttgcggtgat cgcgcgcggc acgcccggac tttcgggcgc cgagctggcg
      421 aatttgttga acgaagcggc gttgctcgct gcgcggcgca acaagaagaa ggtcgaaatg
      481 atcgacgtcg atgatgcgcg tgagaaagtg cagttcggtc gcgagcgtag gcgcgtgatg
      541 gacgacgagg agaagaagct cacggcgtat cacgaagctg gacacgcttt ggtgcaggcg
      601 gtgttggacg acggtcacat gccagtgcac aaggtgacga tcatcccgcg cggccatagc
      661 ttgggcagca cgatgttcat tccgaagaag gacattctta cgcacgcgat gaagcggatg
      721 ttgaaccaga tcgcgatggg cctcggcggt cgcatcgcgg aagaactggt gatgggtgac
      781 gtgtcgagcg gtgcggcggg cgatatcaag caagtgacca agatcgcgcg tcacatggtg
      841 tgcgattggg gcatgagctc gctgggcatg atcgcttacg gcgagagctc ggacacagtt
      901 ttcctcggcc gcgaaatcac gcgcagccag acgtattcgg aggaaaccgc gcgcaagatc
      961 gacgcggagg tttatcgcat cgtcgacgat caatacaaac gcgcgaagga gatcatcacg
     1021 agccgtcgcg atgtgttgga caagatcgcg agcgcgttgc tggagcacga gacgatcgaa
     1081 ggaaagcacg tgcttgaagt gctgcagttc ggcgagatca aatcacccat cgtgcgcgag
     1141 ccggtgaggc agccggaaga caaaggtccg ggcaagaaga cgccgggcaa gtcctcggca
     1201 ccagaagggc ttgcgcctgg aacggcgccg gcgccgaatc cggcgtaaca gccacgaaat
     1261 ggaatcaccg aggcgcggag aactccgcgc cttttttgtg gtcgtaagcg cgcgttttga
     1321 aaaaacatct tggataatcc aagaaacgaa ataacaggcc attatacatg aagatttgct
     1381 gcatcggagc gggctacgta ggcggaccaa ctatggcgat gatcgcgttg aaggcgccgg
     1441 acatagaagt ccgggtcgtc gatatgaacg aggcgcgcat cgcggcatgg aactcggaga
     1501 cgcttccgat ctacgagccc gggctcgacg aagtagtgtg tgaggcgcgc gggcggaatc
     1561 tgtttttcac gacggaagtg cgcgcggcga tcgagtgctc cgacatcatt ttcgtggcgg
     1621 tcaacacgcc gacgaagacg tatggcgtcg gctcgggacg cgcggccgat ctgcggttca
     1681 tcgagtcggt ggcgcggacg atcgcggaag tcgcgacaac gccgaagatc attgtcgaga
     1741 agtcgacgat cccggtgaag acggcggaga cgatcaaaga tatcctcgcg gcgaatacgc
     1801 ggggcgtgaa gttcgaggta ttgtcgaacc cggagttttt ggcggaaggc acggcgatcg
     1861 cggatctgca gatgccggat cgggtgttga tcggtggcga gcgcacgccg ggtggcgagg
     1921 cggcgttgca agtgttggcg gatgtctatg cgcgctgggt gccgcgc
//
LOCUS       DASPLT010001400         1068 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_221119, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001400 DASPLT010000000
VERSION     DASPLT010001400.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1068)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1068)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1068
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_221119"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(167..1018)
                     /locus_tag="VEA63_14275"
     CDS             complement(167..1018)
                     /locus_tag="VEA63_14275"
                     /inference="COORDINATES: protein motif:HMM:NF003703.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HYD85227.1"
                     /translation="MSSFVNISAYKFTPLDDLPVWRERLLQVCRAGDLRGTILLSVEG
                     INLFVAGTRKQIDTLLATLRSIPGLADLTPKESFSAEQPFNRMLVKIKKEIIAFGVPG
                     IDPARRPSPKLPARTLKQWLDEGRPITLLDTRNDYEIRLGTFRGAVPARIEHFREFPA
                     AVKNLPEEMKERPIVMFCTGGIRCEKAGPYLEQAGFKNVHQLDGGILKYFEECGGAHY
                     DGECFVFDRRVGVDPALRETNSVLCFNCQMPLLEAEQRDPRYVPDISCPHCADTSVGQ
                     ARPSLTE"
BASE COUNT          208 a          300 c          365 g          195 t
ORIGIN      
        1 acacgttgcg cgcgagcgcg caacctatcg aagcagggca tgcggagacg cctgccgcta
       61 cgggtaaacg gcggtgattc gaggctgtgg gacgggcttt atgccccgac gatcgggcgc
      121 caacccgtcg gggcgtaaag ccccttcccc cgctgcgggc cggagcttac tcggtgagag
      181 acggacgagc ttggccgacg gatgtatccg cgcagtgggg acaactgatg tcgggcacgt
      241 agcgagggtc gcgctgttcg gcttccaaga gcggcatctg gcagttgaag cacagcacgg
      301 aatttgtttc gcgcagcgcg ggatcgacgc cgacacggcg gtcgaagacg aagcattcgc
      361 cgtcatagtg cgcgccgccg cactcctcga aatatttcaa aatgccgccg tcgagttgat
      421 gcacgttttt gaaaccggcc tgctcgagat agggtccggc cttttcgcag cggatgccgc
      481 ccgtgcaaaa catcacgatc gggcgctcct tcatttcctc cggcaggttt ttcacggcgg
      541 cgggaaactc gcgaaagtgt tcgatgcgcg ctggaacggc gccgcgaaag gtgcccaggc
      601 gaatttcgta gtcgttgcgc gtgtcgagga gcgtgattgg ccggccttcg tcgagccact
      661 gcttcaacgt gcgcgccggg agtttcggac tcgggcggcg ggcgggatcg atgccgggaa
      721 cgccgaaagc gatgatctct tttttgatct tcaccaacat gcgattgaac ggctgttccg
      781 cgctgaagct ttccttgggg gtgagatcgg cgaggccggg aatgctgcgc aacgtggcga
      841 gcagtgtgtc gatctgtttt cgcgtgccgg cgacgaacag gttgatgccc tcaacgctca
      901 acaggatggt gccgcggaga tcgccggcgc gacacacctg cagtagccgc tcacgccaga
      961 cgggaagatc gtcgagcgga gtgaatttat aggcggagat gttgacgaag gaggacacga
     1021 gcaaaggggc ctacgacaac gtccgggcga caggaaaact caaggccg
//
LOCUS       DASPLT010001401         1068 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_181250, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001401 DASPLT010000000
VERSION     DASPLT010001401.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1068)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1068)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1068
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_181250"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..927)
                     /locus_tag="VEA63_14280"
     CDS             complement(<1..927)
                     /locus_tag="VEA63_14280"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HYD85228.1"
                     /translation="MKILHLEDNLHDAELVQLVFSTEWPDCEVKVVSSRDEFVTQLHR
                     GEQDVIVSDFNLLSFNGLEALQLAREKKPDTPFIFLSGTIGEDRAVEALRAGAKDYVI
                     KDRPKRLVPAIKRALDDARHLRERKAAEEQLLRVQRLENIGMLAAGIAHDFNNVLAPV
                     LMAVPLLRERLSEKLDLQILSTVEKSVERGAGLVRQILGFAHGVTGEAHLVQPKHVLR
                     DLVGVMRQTFPRSIEIDEDVANQLWPLKVNPTQLHQVLLNLCVNARDAMPHGGTLHVR
                     ASNRLLDEVSASSIPGAQAGPYLLFEIADTGTG"
BASE COUNT          195 a          264 c          401 g          208 t
ORIGIN      
        1 gccggtgccg gtgtcggcga tttcgaacaa taggtagggg ccggcttggg caccggggat
       61 gctggaggcg ctgacctcgt cgagcaggcg attggacgcg cggacgtgga gcgtgccgcc
      121 gtggggcatg gcgtcgcggg cgttgacgca gagattgagc aacacctggt gaagctgcgt
      181 ggggttgacc ttgagcggcc agagctggtt cgcgacgtct tcatcgatct cgatggagcg
      241 agggaacgtt tgccgcatga cgccgacgag gtcgcggagc acgtgtttcg gttggacgag
      301 gtgcgcttcg ccggtgacgc catgggcgaa gccgagaatt tgacggacga gaccggcgcc
      361 gcgctcgacg cttttttcga cggtggagag gatttgcaga tcgagctttt cgctcaggcg
      421 ctcgcgcagg agcggaacgg ccatgagaac gggagcgagg acgttgttga aatcgtgggc
      481 gatgccggcg gcgagcatgc cgatgttttc gaggcgctgg acgcggagaa gctgttcttc
      541 ggcggctttg cgttcgcgga ggtgacgggc gtcgtcgagc gcgcgcttga tcgcgggcac
      601 gagccgtttc gggcggtcct tgatcacgta atctttggct ccggcgcgaa gggcttcgac
      661 ggcgcggtct tcgccgatgg tgccggaaag aaagatgaaa ggcgtgtcgg gtttcttttc
      721 gcgcgcgagt tggagagcct cgaggccgtt gaacgaaagc aggttgaaat cggaaacgat
      781 cacgtcctgt tcgccgcggt gaagctgggt gacgaattcg tcgcgcgacg aaacgacttt
      841 aacttcgcag tcgggccatt cggtcgagaa caccagctgg accagctcgg cgtcgtggag
      901 attatcttct agatggagaa ttttcatacg tgggagaagc gggaaagcgc cacgtcaggc
      961 agtgaaaagc cgctgtcggc cggcggtgga tcgcgggcaa actgcggcta agatgtgcgg
     1021 cattgcacgc cggttgatca aaaatgcaag cttggtgcgt cgatcgcg
//
LOCUS       DASPLT010001402         1572 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_40906, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001402 DASPLT010000000
VERSION     DASPLT010001402.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1572)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1572)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1572
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_40906"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..420
                     /locus_tag="VEA63_14285"
     CDS             <1..420
                     /locus_tag="VEA63_14285"
                     /inference="COORDINATES: protein motif:HMM:NF013899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreD"
                     /protein_id="HYD85229.1"
                     /translation="VDQLMPGRVAHGEAWEWERLRLELEVRCGGELVLKERLDQSGAE
                     LKGLAALAGSGEGACFANAVLVAEAREMGSEAAWRGAVSALHGDGLWVGVSALRRNGW
                     SIKFVATDGVRLRRGVREARRLLEGNFPRLGCDVRKM"
     gene            complement(572..796)
                     /locus_tag="VEA63_14290"
     CDS             complement(572..796)
                     /locus_tag="VEA63_14290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD85230.1"
                     /translation="MRNPRTLARIAVLAATFTPVVALAHPGHDDHDFTWEFSHLAAHP
                     VATLLCVTVLAAIAWTIWRFLRHPKSRRDS"
     gene            complement(874..>1572)
                     /gene="rph"
                     /locus_tag="VEA63_14295"
     CDS             complement(874..>1572)
                     /gene="rph"
                     /locus_tag="VEA63_14295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012377011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004549 - tRNA-specific ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HYD85231.1"
                     /translation="ITFEANIAPHATGSVLVSFGDTRVICAATIEPNVPTWMKQQRVP
                     GGWLTAEYSLLPYSTHDRKQRDISRGKIDGRTVEIQRLIGRALRAVIDLQKLGQNTLW
                     IDCDVLQADGGTRTASITGAWLAARLAIQKLLDAKKIAENPLTDSVAAVSAGIFAGQP
                     LLDLNYVEDKDAEVDANVVMTGRGQFVELQSAGEEATFSAEQLQSLLALSAKGLKEIS
                     SLQTAFLTKQLLAR"
BASE COUNT          254 a          355 c          666 g          297 t
ORIGIN      
        1 gtggatcagt tgatgccggg gcgggtggcg cacggcgagg cgtgggagtg ggagcggctg
       61 cggctggaac tcgaggtgcg gtgcggcggc gaactggtgt tgaaggagcg actggatcag
      121 agcggcgccg agttgaaggg gctggcggcg cttgcgggtt cgggagaggg cgcgtgtttt
      181 gcgaacgcgg tgctggtggc ggaggctcgc gagatgggga gcgaggcggc gtggcgggga
      241 gcggtgtcag cgttgcacgg cgacggactg tgggtcggcg tgagcgcgct gcggcgcaac
      301 gggtggagta tcaagtttgt ggctacagac ggcgtgcggc tgcggcgggg agtgcgggag
      361 gcgcggcggc tattggaggg gaattttccg cggctggggt gcgatgtgcg gaagatgtga
      421 gcggcgcggg cgctcagtat cgttctcgtt ctctttcttc gttctcgttc tctcgatcag
      481 atcgacgaat cgagaacgag aaagagaacg agaacgaggt ttcgcaacgc gcgaacgtga
      541 tcgcgggatt tagggcacgc gcccttacct tttaagaatc gcggcgcgat ttcgggtggc
      601 ggagaaaccg ccagatggtc caggcgattg cggcgagcac ggtgacgcag aggagggttg
      661 cgaccgggtg tgcggcgagg tgggaaaatt cccaggtgaa atcgtggtcg tcgtggccgg
      721 ggtgggcgag ggcgacgacc ggcgtgaacg tggcggcgag gacggcgatg cgggcaaggg
      781 ttctagggtt tcgcatggcg ggaaattagc aggtcgcggg ccaatgaggg tagttctccc
      841 tgcgctcacg cgcagagcca cggaggaaag cggtcagcgg gcgaggagtt gcttcgtgag
      901 gaaggcggtt tgaagcgagg agatttcctt gaggcctttg gcggagaggg cgaggaggct
      961 ctggagttgt tcggcggaga aggtggcttc ttcgccggcg ctctgaagtt caacgaactg
     1021 gccgcggccg gtcatcacga cgttggcgtc gacctcggcg tccttgtctt cgacgtagtt
     1081 gaggtcgagc agcggctggc cggcgaagat accggcgctg acggcggcga cggagtcggt
     1141 gagggggttc tcggcgattt tttttgcgtc gaggagtttt tggatcgcga ggcgggcggc
     1201 gagccaggcg ccggtgatcg acgcggtgcg cgtgccgccg tcggcttgaa ggacgtcgca
     1261 atcgatccag agcgtgttct ggccgagttt ttgaagatcg atgacggcgc ggagggcgcg
     1321 gccgatgaga cgttggatct ccacggtgcg gccgtcgatt ttgccgcggg agatgtcgcg
     1381 ttgtttgcgg tcgtgggtcg agtaggggag aagtgaatac tcggcggtca accagccgcc
     1441 ggggacgcgt tgctgtttca tccacgtggg aacgttcggt tcgatggtgg cggcgcagat
     1501 gacgcgggtg tcgccgaagg agacgaggac ggagccggtg gcgtgcggag cgatgttggc
     1561 ttcgaaagtg at
//
LOCUS       DASPLT010001403         1781 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_140470, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001403 DASPLT010000000
VERSION     DASPLT010001403.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1781)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1781)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1781
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_140470"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..605
                     /locus_tag="VEA63_14300"
     CDS             <1..605
                     /locus_tag="VEA63_14300"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HYD85232.1"
                     /translation="PRGILDLGCGAGWTSHFLAKAGYTVTGVDISPDAIAIAESNIPE
                     SLRARLTFRVGDYEANSEPGAFDYVLFYDALHHAEDEEAALRTAYAALKPGGAVITLE
                     PGRGHSQTNTSRNAVKQYGVHEKDMPPQYLLKLGRRIGFLRSVVLPLPHDLTRFVYRR
                     DYFAKKLSPVKLCLEKLWGAFRVIGGLSSQAKGSLTIQWR"
     gene            complement(684..>1781)
                     /locus_tag="VEA63_14305"
     CDS             complement(684..>1781)
                     /locus_tag="VEA63_14305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD85233.1"
                     /translation="DTIAKAHADASIAINDAAAAAARDLAAAHTKALDEIDARLEARA
                     AALVSTIEAALAKTASPPLPPAPIDSTSNVDSEPASSESPAVEVASPPKRPRKARRDD
                     EPTEEISAPPIPASAPSPAEIAPEESTPISVSAPTPTTTTSAEISPTPEPPPIPGETI
                     AKIEPVAPVSASPFPITPAPDQTPAPLEEIAPPPAPVADTSLPSTPLESFPATATPSA
                     PPNGEPSAATERSPKKRTPKKTVAPAPELPLPESAPEEFSFSSEDDAPLTSREVMERV
                     ISSDGATRLIATAYIGIGNRLFIRGDGPGLSWEKGVPLQFVSIGKWRWETPDATAPIQ
                     FKLYKNDDTECPGLGTLTLDPGHQQEVTAKF"
BASE COUNT          288 a          461 c          676 g          356 t
ORIGIN      
        1 ctccgcgggg gatactcgac ctcggttgcg gagcaggatg gacctcccac tttctggcga
       61 aggctggcta cacggtgacg ggcgtcgaca tttctccaga cgcgatcgcc atcgccgagt
      121 cgaacatccc tgaaagcttg cgggcgcggc tcaccttccg cgtgggcgat tacgaggcga
      181 acagcgaacc cggcgcgttc gactacgtct tgttttacga tgcgttgcac catgcggaag
      241 acgaggaggc ggcgcttcgc acggcgtatg cggcgctcaa accaggcggg gcggtgatca
      301 cgctggaacc gggtcgcggc cacagccaga cgaacacgtc ccgcaatgcg gtgaagcaat
      361 acggagttca tgaaaaagac atgccgccac agtatctgct gaagttaggc agacgcatcg
      421 gctttctgcg gtcggtcgtc ctgccgctgc ctcacgacct cactcgcttt gtttatcgcc
      481 gggactattt cgcgaagaag ctcagcccgg tgaagctgtg cttggagaaa ctgtggggcg
      541 ctttccgagt gattggcgga ctatccagtc aagccaaagg aagtctgacg atccagtggc
      601 ggtgattttt cgcacgggag aaaccgagtg agattggtaa acgccgtcga ccgaagctcg
      661 gttacgctgc cgggcctgcg tggctagaac ttcgcggtga cttcctgctg gtggccgggg
      721 tcgagggtga gcgtgccgag gccggggcat tcggtatcgt cgttcttgta gagtttgaac
      781 tggatcgggg cggtcgcgtc gggggtttcc caacgccatt ttccgatcga cacgaactgc
      841 aacggaacac ctttctccca actcaggccc gggccgtcgc cgcggataaa gaggcgattg
      901 ccgatgccga tgtaggcggt ggcgatcagg cgcgtggcgc cgtcggaaga aatcacgcgc
      961 tccatcacct cgcgcgaggt gagcggtgca tcgtcttcgg agctgaacga aaattcctcg
     1021 ggagcgcttt ccggtaatgg aagttccgga gctggcgcga ccgttttctt tggcgtgcgt
     1081 tttttcggag agcgctcggt ggcggcagag ggttcgccat ttggcggcgc ggagggcgtc
     1141 gcggtcgcgg ggaatgactc gagaggcgtg gacgggagcg acgtgtcggc gacgggcgcc
     1201 ggcggcggcg cgatttcttc gagcggtgct ggcgtttgat ccggtgcggg tgtgatcggg
     1261 aaggggctgg ctgacacggg ggcgaccggc tcgatctttg cgatggtttc acccgggatc
     1321 ggaggcggtt cgggggtggg tgaaatttcg gctgacgttg tggtcgttgg tgtcggagcg
     1381 gagacagaaa tgggagtgct ctcttcagga gcgatttcgg ccggcgaagg agcggacgca
     1441 gggatcggtg gagcggagat ttcttcggtc ggctcgtcgt cgcggcgggc tttgcgcggg
     1501 cgtttcggag gggaggcgac ttccacggcg ggggattcgg acgacgctgg ttcgctgtcg
     1561 acgttgctgg tggaatcgat cggagcaggc ggaagcggag ggctggcggt tttggcgagc
     1621 gcggcttcga tggtggaaac gagcgcggcg gcgcgggctt cgaggcgggc gtcgatttcg
     1681 tcgagtgctt tggtgtgggc ggcggcgagg tcgcgagcag cggcagcggc ggcgtcgttg
     1741 atggcgatcg aggcgtcggc gtgcgctttg gcgatggtgt c
//
LOCUS       DASPLT010001404         1875 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_240938, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001404 DASPLT010000000
VERSION     DASPLT010001404.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1875)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1875)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1875
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_240938"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1837)
                     /locus_tag="VEA63_14310"
     CDS             complement(<1..1837)
                     /locus_tag="VEA63_14310"
                     /inference="COORDINATES: protein motif:HMM:NF021606.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2339 domain-containing protein"
                     /protein_id="HYD85234.1"
                     /translation="MGAKLFAWFGGLAALLGVAFFVKYSFEHDLIPPEVRVALGFLFA
                     LSLVIGGLKIDRTRYGITAQTLCATGVVSLYAVTFACNAVYHFAPFNPVVTFLVMVLI
                     TATAFLLAVRMNAQVVALLGLLGGFLTPILVSTGKDNPFGLFGYIALLDVGLAAVALN
                     RRWHYLVALAAAGTVFMELAWTGKFFSAPRAPLAMAIALAFSALFGAIAELGRRWQRP
                     SHHATWSAFALPLVALGFAFYFLSFETIATRAGLFLGFVFAADIFLLLLAWRDDAARR
                     VHLVAGAVVFTLLSLWTAGRLTTELLPWALGAALGFAALHTAFPLLLERARPTARPGV
                     LSQIFPPLTLVLLLIPLFKLDEISLLFWPAVLLLDALAVALALLSGSLIALALVLVLT
                     LATAGVAILRTPIDGDISLSLLFIIAVFAVFFFAAGLFLARKLGPRLLADAGERGVSD
                     RLFGDARAQVPAFGALLPFVLLVIMSQRLAFADPSPLFGLALLLCLLVFGLTRLFVIE
                     WLPACALAGVAALEYVWHTRSFSSAQPVTPLLWYLGFHALFAAFPFVFRKHFAALTGP
                     WAIAALSGAVQFPLVYNVVLQAWPNNIMGLLPAGFALPPLLSLVII"
BASE COUNT          463 a          553 c          653 g          206 t
ORIGIN      
        1 ggatgatcac gagactcagc aggggaggca gtgcgaaacc cgcggggagg agccccatga
       61 tgttgttggg ccaagcctgg agaacgacat tgtagaccag cggaaactgc accgcgccac
      121 ttagcgcagc gatcgcccaa gggccggtga gtgcggcgaa atgtttccgg aaaacgaacg
      181 gaaacgccgc gaacagcgcg tgaaagccga gataccaaag cagcggcgtc accggttgcg
      241 ctgacgagaa cgatcgcgtg tgccacacat attcgagcgc ggcgacgccg gcgagcgcgc
      301 aagccggcag ccactcgatc acaaagagcc gcgtgagtcc gaacaccagc aggcagagca
      361 gcaatgcgag gccgaagagc ggcgacggat cggcgaacgc gagccgctga ctcatgatca
      421 cgagcagcac gaatggcagc agcgcgccaa atgcggggac ctgcgctcgc gcgtcgccaa
      481 acagccggtc ggagactccc cgttccccgg cgtccgcgag cagacgcgga ccgagtttgc
      541 gcgcgagaaa cagtcccgcg gcgaagaaaa agaccgcgaa caccgcgatg atgaaaagca
      601 gcgagagcga aatatcgccg tcgatcggcg tgcgaaggat cgcaaccccc gccgtggcaa
      661 gcgtcagcac caacacgagc gccagcgcga tcagcgagcc gctcagcaga gcaagggcga
      721 ccgcgagcgc gtcgaggagc aggaccgcgg gccagaacag cagcgagatt tcgtcgagct
      781 tgaagagcgg gatcagtagc agcacgagcg tgagcggcgg aaaaatctgg ctcagcacac
      841 ccggtcgggc ggtgggccgc gcgcgttcca gcaggagggg aaacgccgtg tgcaatgcgg
      901 cgaagcccag cgcggcgcct agtgcccagg gaagaagttc ggtcgtgagc cgtccggccg
      961 tccagagcga gagcagcgtg aagaccacgg cgccggcaac cagatgaacc cgccgcgctg
     1021 cgtcatctcg ccaggcaagc aggagcagaa aaatatccgc ggcgaaaacg aagccgagaa
     1081 aaagtccggc ccgtgtcgcg atcgtttcga acgaaaggaa ataaaacgcg aaaccgagcg
     1141 ccacgagggg aagtgcgaag gcggaccagg tggcgtgatg cgagggccgt tgccagcgtc
     1201 ggccgagctc ggcgatggcg ccgaagagtg cggagaacgc gagggcgatc gccatcgcca
     1261 gcggagcacg cggcgcggaa aagaattttc ccgtccacgc aagttccatg aacaccgtgc
     1321 ccgccgcggc gagggccacg agatagtgcc agcgacgatt cagggcgacg gcggcgagac
     1381 cgacgtcgag caaagcgatg taaccgaaga ggccgaatgg gttgtctttg ccggtcgaga
     1441 caaggatggg agtaagaaaa ccgcccagca gcccgagcaa ggcgacgacc tgagcattca
     1501 tccgcaccgc gaggagaaag gccgtcgcgg tgatcaaaac catcacgaga aacgtgacca
     1561 cgggattgaa cggtgcgaag tggtagacgg cgttgcacgc aaaggtgacg gcgtagaggc
     1621 tgacgacgcc ggtcgcgcac agcgtctgcg ccgtgatgcc atagcgcgta cggtcgatct
     1681 tgagtccgcc gatcacgagc gagagcgcga acaggaatcc cagagccacg cggacctcgg
     1741 gcgggatcag atcgtgttcg aaggagtatt tgacgaagaa ggcgacgccg agcagcgcgg
     1801 cgagaccgcc gaaccacgcg aaaagtttcg cgcccatgaa ttgctcccaa tcgatccggg
     1861 cgcgagcccg cggcg
//
LOCUS       DASPLT010001405         1075 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_56221, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001405 DASPLT010000000
VERSION     DASPLT010001405.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1075)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1075)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1075
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_56221"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..611
                     /locus_tag="VEA63_14315"
     CDS             <1..611
                     /locus_tag="VEA63_14315"
                     /inference="COORDINATES: protein motif:HMM:NF013630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HYD85235.1"
                     /translation="LQPNARQAVYVRGRIDVATREFARTLPAHPLESQTDRIEVFEQV
                     NRLQRENEQLKMELASLRGGAPTTVRTRVPLVDEPRATAERGTDTVQRPFITAYATPA
                     PVPVEDSPVSLAPEDFEETNVNEPAIQPAPLGHGERTVPTRPNAPATSGRKHLVVKGD
                     TLFSLAQRYYGNRSRWRDLYAANRDVLPSENSLRIGMELRIP"
     gene            788..>1075
                     /locus_tag="VEA63_14320"
     CDS             788..>1075
                     /locus_tag="VEA63_14320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007358844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HYD85236.1"
                     /translation="MPRTSQRTSRFTESIIREMSRVAKTHGAINLAQGFPDWDPPAAL
                     VQAAKDAMDAGRHQYAVTWGSPELRDALGGKLTRFLGTPVDAERELVVTCGA"
BASE COUNT          199 a          340 c          351 g          185 t
ORIGIN      
        1 agctgcagcc caacgcgcgg caggccgttt acgtgcgcgg gcggatcgac gtggcaacgc
       61 gcgaattcgc gcgcacgctg ccggcgcatc cgctcgaaag tcagacggac cggatcgagg
      121 tgttcgagca ggtcaaccga ctgcagcgcg agaacgagca gttgaaaatg gagctcgcct
      181 cgctgcgcgg cggcgcgccg accacggtgc gcacacgggt tccgctcgtg gacgagccac
      241 gcgcgactgc cgagcgcgga actgacaccg tgcaacgtcc gttcatcacc gcctacgcga
      301 cgcccgcgcc ggttccggtg gaggattcgc cggtgtcgct cgcgcctgag gacttcgaag
      361 agaccaacgt gaacgagccc gcgattcagc ctgcgccgct cggccacgga gaacgcaccg
      421 ttccgacgag accgaatgca ccggccacga gcggccgcaa gcatctcgtg gtgaagggcg
      481 acaccttgtt cagtctcgcg cagcgctatt acggcaaccg ctcgcgctgg cgcgacctct
      541 acgcggcgaa tcgcgatgtg ctgccgagcg aaaactcgct gcgcatcggc atggagctgc
      601 gcatcccgtg atgagaaccg cgaatggaca cgaattcaca cgaatgtcag aggagtcatg
      661 tcgatgtatc cgacatgatc acacgacgat cgaacagtga ctcgtctacc accacgcgtt
      721 ccttctgatt tctctttcgt gttcattcct ggccattcgt ggttgaactg agttgttacg
      781 gccgaatatg ccccgcacgt ctcaacgcac gtctcgtttc accgagtcga tcattcgcga
      841 aatgtcgcgc gtcgcgaaaa cgcacggcgc gatcaatctc gcgcagggtt ttcccgattg
      901 ggatccgccg gcggcgctcg tgcaggcggc gaaggacgcg atggacgcgg ggcgtcacca
      961 gtatgcggtg acgtggggtt cgccggagtt gcgcgatgcg ttgggcggaa agctgacgcg
     1021 gtttcttggc acgccggtcg acgccgagcg cgagttggtg gtcacctgcg gcgcg
//
LOCUS       DASPLT010001406         1423 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_282390, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001406 DASPLT010000000
VERSION     DASPLT010001406.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1423)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1423)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1423
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_282390"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..934)
                     /locus_tag="VEA63_14325"
     CDS             complement(<1..934)
                     /locus_tag="VEA63_14325"
                     /inference="COORDINATES: protein motif:HMM:NF037031.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M14-type cytosolic carboxypeptidase"
                     /protein_id="HYD85237.1"
                     /translation="MMRARQAYVGALAWAMFACGLTFASDSAPRDSREPILFNTAFEG
                     ASLGEIEAIDATTFRLHVRGQQDERGRNRQATWYFFRMDRVAGREITLTFTDWVGEYN
                     DIPGTVPMSAKLRPWISEDQKHWRPIEQMVWDADRKEATVTVRAKSDRVWLAHLQPYS
                     HSRLLGLLDELNQSAAARIEVIGKTVLGRDLHLITVTDFAQPNAGKKTVWLQARQHAW
                     ETGTSFVMEGALRFITSDDPRARELRRHTIFKFTPMLDPDGCANGGVRFNANGYDLNR
                     HWERVDLRDPAALRAMPESWYLKKAIVGHLHAGGG"
     gene            complement(931..>1423)
                     /locus_tag="VEA63_14330"
     CDS             complement(931..>1423)
                     /locus_tag="VEA63_14330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD85238.1"
                     /translation="NRQTGTNNYRISCYIRRKPTGELYWHVRGEAPQPFRTVDWDLFN
                     DEVPVPVGRWFKVEVFFRHADDGMMWLAIDGRQVAFRRGRTQHPDNPLPIGFWSPFKL
                     YLGPEWLARGAPHQWIDDVEFWPDFPADATPRDEARANRSTFTPEIAARASASSPGPR
                     ATP"
BASE COUNT          274 a          492 c          396 g          261 t
ORIGIN      
        1 tgcctccgcc ggcgtgcaga tggccaacaa tcgctttttt cagataccag ctttccggca
       61 tcgcgcgcaa cgccgccgga tcgcgcaaat ccacgcgctc ccagtggcgg ttgagatcgt
      121 agccgttggc gttgaaccgc acgccgccat tcgcacagcc gtctggatcg agcatcggcg
      181 tgaatttgaa aatcgtgtgc cggcgcagtt cgcgtgcgcg cggatcgtcc gacgtgatga
      241 aacgcaacgc cccctccatc acgaacgacg tgcccgtttc ccacgcgtgc tgccgcgcct
      301 gcagccacac ggttttttta cccgcattcg gctgcgcgaa atccgtcacc gttatcaagt
      361 ggagatcgcg acccagcacg gtctttccga tcacctcaat gcgcgccgcg gccgactgat
      421 tcaactcgtc gagcaagccc aacaagcgcg aatgggaata aggctgcaaa tgagccagcc
      481 acacgcgatc gcttttcgcg cgcaccgtga ccgtcgcctc cttccggtcc gcatcccaca
      541 ccatctgctc gatcggcctc cagtgttttt gatcttcgct gatccacgga cgaagtttcg
      601 cgctcatcgg caccgtgcct ggaatatcgt tatactcacc cacccaatcg gtgaacgtca
      661 gcgtgatttc gcggccggcg acgcggtcca tccggaaaaa ataccacgtc gcctgtcggt
      721 tgcgtccgcg ctcgtcctgc tgtccgcgca catgcaggcg aaacgtcgtc gcgtcgatcg
      781 cctcgatttc gccgagcgaa gcgccctcga acgccgtgtt gaacagaatt ggctcgcgcg
      841 aatcgcgcgg cgcggaatca gaagcaaaag tcagcccgca cgcgaacatc gcccatgcga
      901 gcgctccgac atacgcttgc ctcgcgcgca tcatggcgtg gcccgtggtc cgggcgacga
      961 agccgacgct cgtgcggcga tctccggcgt aaacgtcgac cgattcgcgc gtgcttcatc
     1021 ccgcggcgtc gcatctgcgg gaaaatccgg ccagaactcc acgtcatcga tccactgatg
     1081 gggcgcgccc cgcgccagcc attcgggacc gagatagagc ttgaacggcg accagaaacc
     1141 gatcggcaac ggattatcgg gatgctgcgt tcgccctcgc ctgaacgcca cctgacggcc
     1201 gtcgatcgcg agccacatca tcccgtcgtc cgcgtgacga aagaaaacct ccaccttgaa
     1261 ccagcgaccg accggcaccg gcacctcgtc gttgaacaaa tcccaatcga ccgtccggaa
     1321 aggctgcggc gcctcgccac gcacgtgcca gtaaagctca cccgtcggct tccggcgaat
     1381 gtagcatgag atgcgataat tgttcgttcc cgtctggcgg ttg
//
LOCUS       DASPLT010001407         1452 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_118382, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001407 DASPLT010000000
VERSION     DASPLT010001407.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1452)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1452)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1452
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_118382"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..377)
                     /locus_tag="VEA63_14335"
     CDS             complement(<1..377)
                     /locus_tag="VEA63_14335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HYD85239.1"
                     /translation="MTEKFYPELSARFAHFLQRIRGRKIAVIGHARPDGDCIGSQVAV
                     ARVLRALGHEVICVNGDVVPRRLQFLVTGMTFLRTDDVVNDPTDYAAVFVDCADHARA
                     GERLKARFPAPVANIDHHLSNVG"
     gene            complement(374..784)
                     /gene="rbfA"
                     /locus_tag="VEA63_14340"
     CDS             complement(374..784)
                     /gene="rbfA"
                     /locus_tag="VEA63_14340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HYD85240.1"
                     /translation="MSNRTLRVNELIQRELSDILRKRYQSESVTITITEVRVAPDLRD
                     ARVFISVMGSDEDRERKLRWLRTHARDIREELGRRIVLKYLPRFEYVADNSMEKGTRI
                     LQMLDEIDRQSPLPEPTEDGEAAAEEPPEDRPAP"
     gene            complement(858..1184)
                     /locus_tag="VEA63_14345"
     CDS             complement(858..1184)
                     /locus_tag="VEA63_14345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD85241.1"
                     /translation="MMADMLEPDLKETKTGAAEVRQVFPLAKGFVAGCLVTEGKITRN
                     AAARLRRGREIVHEGKIGTLKRFKDDANEVRAGLECGIKLDDFNGYQAGDVIEVYEVQ
                     KVRASL"
     gene            complement(1181..>1452)
                     /locus_tag="VEA63_14350"
     CDS             complement(1181..>1452)
                     /locus_tag="VEA63_14350"
                     /inference="COORDINATES: protein motif:HMM:NF023413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HYD85242.1"
                     /translation="IIKTDVFGSSEAVRTILEGIKGSKVSLEIVSTDVGLVTKNDVLM
                     ASAAGAVIIGFNTKLENGVTPLAKHHGVRVETYSIIYELGDKVRE"
BASE COUNT          263 a          479 c          426 g          284 t
ORIGIN      
        1 tagccgacat tcgagagatg atggtcgatg ttcgccacgg gcgccggaaa gcgtgccttc
       61 aaccgctcgc ccgcgcgtgc gtggtcggcg caatcgacga acaccgccgc gtaatcggtc
      121 ggatcgttca ccacatcgtc cgtgcgcagg aacgtcatgc ccgtcaccaa aaactggaga
      181 cgtcgcggca cgacgtcgcc gttgacgcaa atcacctcgt ggccaagcgc acgcagcaca
      241 cgcgccaccg ccacctgcga accgatgcaa tcgccgtcgg gccgcgcatg tccgattacc
      301 gcgatcttcc gtccgcgaat tcgttggagg aagtgcgcga agcgcgcgga aagctccgga
      361 taaaacttct ccgtcatggc gcaggccgat cctccggagg ctcctcggcg gccgcctcac
      421 cgtcctcggt cggctccgga agcggcgact gccggtcgat ttcgtccagc atctgcagga
      481 tgcgcgtgcc cttctccatc gagttgtcgg ccacgtattc gaaccgcggg agatatttca
      541 gcacgatgcg acggccgagc tcctcgcgaa tatcgcgcgc gtgggtgcgc aaccagcgga
      601 gcttccgttc ccggtcttca tccgatccca tcaccgagat gaacacgcgc gcatcccgca
      661 gatcgggcgc gacccgcact tccgtgatcg taatcgtcac ggactcgctc tggtagcgct
      721 tgcgcaggat gtcgctgagc tcgcgctgga tcagctcgtt gacgcgaagg gtgcggttcg
      781 acattgccga tttcggattg gcgattgccg actcaatcta gcgccaatcg gaaattggca
      841 atcggcaacc ggctttctca gagcgaggcg cgaaccttct ggacttcgta aacctcgatg
      901 acgtcgcccg cttggtagcc gttgaaatcg tcgagcttga taccgcactc gagacccgcg
      961 cgcacttcgt tggcgtcgtc cttgaaacgt ttgagcgtgc cgatcttgcc ttcgtgcacg
     1021 atctcgcgac cgcgacgcaa acgcgcggcg gcattgcggg tgatcttgcc ttcggtcacg
     1081 agacagccgg cgacaaaacc cttggcgagc gggaacacct ggcgaacctc ggcggcgccc
     1141 gtcttcgttt ccttgagatc gggctcgagc atgtcggcca tcattcgcgc accttgtcgc
     1201 ccagctcgta aatgatcgag taggtctcga cgcgcacacc gtggtgcttc gcgagtggcg
     1261 tcacgccgtt ctcgagcttc gtgttgaagc cgatgatcac cgctcccgcc gcactcgcca
     1321 tgagcacgtc gttctttgtc accaagccca catcggtcga aacgatctcg agcgaaacct
     1381 tcgagccctt gatgccctcg aggatcgtgc gcaccgcttc cgatgagccg aacacgtcgg
     1441 tcttgatgat ga
//
LOCUS       DASPLT010001408         1779 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_221175, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001408 DASPLT010000000
VERSION     DASPLT010001408.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1779)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1779)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1779
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_221175"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(98..1213)
                     /locus_tag="VEA63_14355"
     CDS             complement(98..1213)
                     /locus_tag="VEA63_14355"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HYD85243.1"
                     /translation="MRPLPLAAFSALALVLAPGPLAPIASAQSRPKMDFESYNPPSTL
                     VVPEHPLTRAKFPFIDVHNHQWNMRSQDLAALTAQMDELNLAIMVNLSGRGGTELEQI
                     MANVKANAPRRIAVFTNIDFSGYGSPGWIDAAVARLERDVHHGAKGLKFFESFGLTEK
                     DNEGKRIPVDDPRMDPIWRKCAELKIPVLIHTAAPKSFWDPVDANNERWLELVTHPRR
                     QRSATHPAPWAEAIGEQHTVFRRHPNTTFIAAHFGWHAHDLAKLGQLLDELPNMNVEM
                     GAIIAELGRQPRAAKKFFERYADRILFGKDSWVPPEYTTYFRVLETEDEYFPYHKKYH
                     AFWAMYGLGLSDDVLKKVYYKNALRLIPGLDPTLFPE"
BASE COUNT          325 a          452 c          669 g          333 t
ORIGIN      
        1 aaaatcaagc gggcaggcgc ggaggcctgc cgctacaccg gacgtgttgg gcggctgcga
       61 atgggaattc gcgcaggcgg ggcgccgagt ttgcgaacta ctcggggaag agggtggggt
      121 cgaggccggg gatcaggcgg agagcgtttt tgtaatagac ctttttcagc acgtcgtcgg
      181 agaggccgag gccatacata gcccagaagg cgtggtattt tttgtgatac ggaaagtatt
      241 cgtcctcggt ctcgagcacg cggaagtaag tcgtgtattc gggaggcacc caactgtctt
      301 tgccgaagag aatacggtcg gcgtagcgtt cgaagaattt tttcgcggcg cgcggctggc
      361 gtccgagttc ggcaatgatg gcacccatct cgacgttcat gttgggcagc tcgtcgagga
      421 gctggccgag tttcgcgagg tcgtgggcgt gccagccgaa gtgcgcggcg atgaaggtcg
      481 tgttgggatg acggcggaaa acggtgtgtt gctcgccgat ggcttcggcc cagggcgcgg
      541 gatgcgtggc gctgcgttgg cggcgcgggt gagtgacgag ctcgagccag cgttcgttgt
      601 tggcgtcgac gggatcccag aacgatttcg gcgcggcggt gtggatgagg accgggattt
      661 tcagctcggc gcattttcgc cagatcgggt ccatgcgcgg atcgtcgacg gggatgcgtt
      721 tcccttcgtt gtccttttcg gtgaggccga agctttcgaa aaacttcagg cctttggcgc
      781 cgtggtggac atcgcgttcg aggcgggcga cggcggcgtc gatccagccg ggcgagccgt
      841 agcccgagaa atcgatgttg gtgaaaacgg cgatgcggcg cggagcattg gccttcacgt
      901 tggccatgat ttgctcgagc tcggtgccgc cgcgaccgct gaggttgacc atgatcgcca
      961 ggttgagctc gtccatttgg gcggtgagcg cggcgaggtc ctgcgaacgc atgttccact
     1021 ggtgattgtg gacatcgatg aacgggaact tggcgcgcgt gagggggtgt tcgggaacga
     1081 cgagggtcga tggagggttg taggactcga agtccatttt cggccgcgat tgggccgacg
     1141 cgatgggagc gagtgggccg ggtgccaaga cgagcgcgag ggcgctgaat gcggcgaggg
     1201 ggaggggacg catgcgcaga ctgacgacgc cggcgcgaaa ccgttgcgcc ggcctcgtcg
     1261 gctccggact ggccttcgca acggttcaaa acgtcgaaca gtgaactgtc gcatatgcga
     1321 cataaaacag ctcacctcgt ggcggaacgt gggcgtgggg agcgtgaatt tgagcggcga
     1381 aacacgacgg ccaggcgcga cgtgtgcagt gacgcgcgcg aggctcgctc gagcgctaca
     1441 agcaaaacgc gagcggttcg atgtcgcata tgcgacagct gaatgctcgg tcgggggcgc
     1501 ggggccggga ggcggagggg acgtaagagc gactctatga acagatcgga cccgagccgc
     1561 tgattctcga aggcgcgatt gcccgcgacg gccgcgagcg acgcatcctg cacctgcgac
     1621 gacggctcac ttgttgagct gctggagctt ttcgcggaaa aagcggcctt ggcgggtggg
     1681 cgtaaaatcc cagtcttcga tgagctgcgg cgaccagtgg gcgtcgaaaa cccagggcgt
     1741 ccaggagatg ccgcgtttct cgaagaaagc gatgatcgc
//
LOCUS       DASPLT010001409         2950 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_77386, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001409 DASPLT010000000
VERSION     DASPLT010001409.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 2950)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2950)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2950
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_77386"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..672
                     /locus_tag="VEA63_14360"
     CDS             <1..672
                     /locus_tag="VEA63_14360"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HYD85244.1"
                     /translation="VVPAIAQAARAATKALAKIAAGKSGPFAGEDEESLTYRDGFLMN
                     EKGRRASIADVLTAASLERVQGEGESAEGEEAERYTFRCFGAHFVEVRWDLGIARLRV
                     SRVVSAFDVGRVINRKTAANQIYGSILMGIGMARLEESVYDSRDGHVVTDNVADYLMP
                     VHADTPEMDIHFLDIPDPHVGEFGARGIGEIGITGMGGAILNAIYHATGKRVRELPVR
                     IEALL"
     gene            complement(824..2560)
                     /locus_tag="VEA63_14365"
     CDS             complement(824..2560)
                     /locus_tag="VEA63_14365"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007363856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative manganese-dependent inorganic
                     diphosphatase"
                     /protein_id="HYD85245.1"
                     /translation="MTPPVPPTNATTYIIGHKNPDADSICSAIAYAAFKEARGERGYV
                     AARCGNTNARIDTILSRFKQPLPLYLSDVSPRVRDLMVKNVIALDENATCAEALAIFD
                     RHDIRVVPVTSRKGIVGTISLSTLGGVFIPRLSEPRLLRLVRTSLGNIARTLEGRALH
                     LVDDRSIQELYVRLGTMDISSFWSISIRENIPPEQSIIIVGDRADIQRRAIEIGVRAI
                     VVTSNLGIDEENLALAKEKGTSIIISPYDSATTSWIVRTASTLERIVDREFTTVDADT
                     RIADVRKRFSATSPHALLVTDDHGKLEGILTKTDLLRPTQTRLVLVDHNEMSQAVTGA
                     DEVTITEIIDHHRLGAINTQQPILFLNEPVGSTCTIVADLFRREGLQPSPEIAGIMMS
                     GLISDTLHLNGPTTTGKDAILLAWLAEISGVNSKQLADEIFNSGSVILANPPATIVRS
                     DYKIYEEEGVRFSVSQVEELGFGNFWQQAKPIATALQELREEERLTFAALLVTDINTQ
                     NSLLLVKADPSFIERISYAHVEQDEIFDLPGVVSRKKQLIPYLTSVLKEMAADGSLAA
                     AVSAPPFRKKKR"
     gene            complement(2667..>2950)
                     /locus_tag="VEA63_14370"
     CDS             complement(2667..>2950)
                     /locus_tag="VEA63_14370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD85246.1"
                     /translation="PTTPIAFTRGLQANGSWDIASGVYKDTGGFVVFLGGNVVFYNST
                     VDAFTSPTTGRKVTDIRQAIPANARIYAITTSAGPVLGTPNGTAAQPDN"
BASE COUNT          527 a          729 c         1120 g          574 t
ORIGIN      
        1 gtggtgcccg cgatcgcgca agccgcgcgg gcggcgacga aggcgttggc aaagattgcg
       61 gcgggcaaga gcggaccgtt cgcgggcgag gatgaggaat cgctcacgta tcgagacgga
      121 tttttgatga acgaaaaggg aaggcgtgcg agcatcgctg acgtgctcac cgcggcgagc
      181 ctggagcggg tgcaaggcga aggagaaagc gcggaaggcg aggaggcgga gcgttacacg
      241 tttcggtgct tcggtgcgca ttttgtcgaa gtgcgctggg atctgggaat cgcgcggctg
      301 cgggtgtcgc gcgtcgtgag cgcgttcgac gtgggacgcg tgatcaaccg gaaaaccgcg
      361 gcgaatcaga tttacggctc gatcctgatg ggcatcggaa tggcgcggtt ggaggagtcg
      421 gtttacgact cgcgcgacgg gcacgtggtg acggacaacg tcgccgatta tctgatgccg
      481 gtgcatgccg acacgccgga gatggatatc cattttctgg atataccgga cccgcatgtc
      541 ggcgagtttg gcgcgcgcgg gatcggcgag atcggcatca ctgggatggg cggagcgatc
      601 ttgaacgcga tctatcatgc gaccggcaag cgcgtgcgcg agttgccggt gcggatcgaa
      661 gcgttgctgt gagcgtcgcg gcagattggc gtagcggcag gcctccgtgc gtgcctgctc
      721 ttcgcttcga gtgtagatgg tgcgcttgcc gcgccacatt gcgcgcgagc gcgcaaccta
      781 cgaacgagca ggcaggcgcg gagacctgcc gctacggcgg cggttagcgt ttctttttgc
      841 ggaagggcgg ggcggagacg gcggcggcga gggagccgtc ggcggccatt tcttttagca
      901 cgctcgtgag atacgggatg agctgtttct tgcggctcac caccccgggg agatcgaaaa
      961 tttcgtcctg ctccacgtgc gcgtaggaaa tacgttcgat aaacgaagga tcggctttca
     1021 ccaacagcag ggagttttgc gtgttgatgt cggtcaccaa caacgcggcg aaggtgaggc
     1081 gttcttcttc gcgaagctcc tgaagcgcgg tggcgatcgg tttcgcttgt tgccagaaat
     1141 tgccgaagcc gagttcttcc acctgcgaga cggagaagcg gacgccttct tcttcgtaga
     1201 ttttgtagtc ggagcgcacg atcgtcgcgg gcggattcgc gaggatgacg gagccggagt
     1261 tgaagatttc gtcggcgagt tgtttcgagt tcacgccgga gatttcagcg agccacgcga
     1321 gaaggatcgc gtccttgccg gtcgtggtcg gaccgttgag gtgcagcgtg tcggaaatga
     1381 ggccggacat catgatcccg gcgatctcgg gcgagggttg caggccttcg cgacggaaga
     1441 ggtcggcaac gatcgtgcag gtcgagccaa cgggctcgtt gagaaacaga atcggctgct
     1501 gcgtgttgat ggcgccgagc cggtgatggt cgatgatttc ggtgatcgtg acttcgtcag
     1561 cgccagtgac ggcttgcgac atctcgttgt gatcgacgag cacgagtcgc gtttgcgtgg
     1621 ggcgaaggag gtcggtcttg gtgagaatgc cctcgagttt gccgtggtcg tcggtgacga
     1681 gcagcgcgtg cggcgacgtg gcggagaaac gcttgcggac gtcggcgata cgcgtgtcgg
     1741 catcgaccgt ggtgaactcg cgatcgacga tgcgttcgag ggtcgaggcg gtgcgaacga
     1801 tccacgacgt tgtcgcggaa tcgtaggggc tgatgatgat gctggtgcct ttttccttcg
     1861 cgagggcgag gttctcttcg tcgatgccga gattgctggt gacgacgatg gcgcgcacgc
     1921 cgatctcgat cgcgcggcgt tggatgtcgg cgcgatcgcc gacgatgatg atcgattgct
     1981 cgggcggaat gttttcgcgg atggagatgc tccagaagga gctgatgtcc atcgtgccga
     2041 ggcggacgta gagttcttga atcgaacgat cgtcgacgag atgaagcgcg cggccttcaa
     2101 gcgtgcgggc gatgttgccg agcgaggtgc ggacgagacg gaggaggcgc ggctcgctga
     2161 ggcgcgggat gaagacaccg ccgagcgtcg agagcgagat cgtgccgacg atgcctttgc
     2221 gcgaggtgac ggggacgaca cggatgtcgt gtcgatcgaa gatcgcgagc gcttccgcac
     2281 atgtggcgtt ttcgtcgagt gcgatgacgt ttttcaccat caagtcgcgg acgcgcggac
     2341 tgacgtcgct gaggtagagc gggagcggct gcttgaagcg cgagaggatg gtgtcgatgc
     2401 gcgcgttggt gttgccgcaa cgcgccgcca cgtagccgcg ttcgccgcga gcttccttaa
     2461 aggcggcgta ggcgatcgcc gagcagatcg agtcggcgtc gggatttttg tggccgatga
     2521 tgtaggtcgt ggcgttggtc ggcggaacgg gaggcgtcat gcgaagcgga gcgcgaacgt
     2581 gtggataatt ggcgtgaagt gaatgaaaaa gcccgaccgg tcagggtcgg gccaatgagg
     2641 acagtgcgaa acggcgagtg gttcgctcag ttgtcgggct gtgcggcggt gccgttgggt
     2701 gtgccgagca cgggaccggc cgaggtggtg atggcgtaga tgcgagcgtt ggcggggatc
     2761 gcctggcgga tgtcggtgac tttgcgaccg gtggtcggcg aggtgaacgc gtcgacggtg
     2821 gagttgtaga acacgacgtt gccgccaaga aaaacgacga agccgccggt gtctttatac
     2881 acgccgctgg cgatatccca ggagccgttg gcctgcagtc cgcgagtgaa ggcgatcggc
     2941 gtggtgggcg
//
LOCUS       DASPLT010001410         1046 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_262260, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001410 DASPLT010000000
VERSION     DASPLT010001410.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1046)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1046)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1046
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_262260"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1046
                     /locus_tag="VEA63_14375"
     CDS             <1..>1046
                     /locus_tag="VEA63_14375"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HYD85247.1"
                     /translation="EAGKLELRPAPFNLPQLLRDLFTALEARAIQKQLHFSLELAADL
                     PATVVGDAQKLRQVLDNLLGNAVKFTPRGAVVLRAFPTGGRIAFAVEDTGAGIPEKEQ
                     ANLFQPFHQPADGRPPEPGTGLGLAIARRLVALMGGELTFETTPGRGSTFSFAVTLEA
                     LASGSHDAEPVARPPIGYRGPRRRLLVVDDVAVNRSVLVELLQPLGFELREAASGAEA
                     LALMPEFKPHLVFLDLRMPGMDGLELTRRLRALPVSGEPLVLVAMSASVLSFNRDSAL
                     EAGCDDFLPKPFREPDLIAKLSMHLRIEWRHGLTAHPFSGDKCSAPASTVELGTLLET
                     ARRGEIRALREQLA"
BASE COUNT          167 a          391 c          295 g          193 t
ORIGIN      
        1 tcgaagcggg aaaactcgag cttcgcccgg cgcctttcaa ccttccgcaa ctcctgcgcg
       61 atctgttcac cgcactcgaa gcccgcgcga ttcagaaaca gcttcatttc agtctcgagc
      121 tcgccgccga cctgcccgca accgtcgtcg gcgacgcgca aaaactccgc caggtgctcg
      181 acaatcttct cggcaacgcc gtgaaattca ccccgcgtgg cgccgtcgtg ctgcgcgcat
      241 ttcccaccgg cggacgcatc gccttcgccg tggaagacac cggcgccggc atccccgaaa
      301 aagaacaggc caatcttttt caaccgtttc accaacccgc cgacggccgt ccgcccgagc
      361 cgggaaccgg cctcggtctc gcgatcgccc gacggctcgt cgcgctcatg ggcggcgaac
      421 tgactttcga aaccacgccc ggccgtggca gcacgttttc ctttgccgtg acgctcgaag
      481 cgctcgccag cggttcgcac gacgccgagc cggtggcgcg tcctccgatc ggctaccgcg
      541 gcccgcggcg acgactgctc gtcgtcgacg acgttgccgt caaccgctcc gttctcgtcg
      601 aactccttca accgctcggc ttcgaacttc gcgaagccgc gagcggtgcg gaagcgctcg
      661 cgctcatgcc cgagttcaag ccgcatctgg tgttcctcga tttgcgcatg cccggcatgg
      721 acggcctcga actcacgcgc cggctccgtg cgctgccagt cagcggcgaa ccgctcgtgc
      781 tcgttgcgat gtccgcttcc gttttgtcct tcaaccgtga cagcgcgctc gaggccggtt
      841 gcgacgattt tctgccaaag ccgtttcgcg agcccgatct cattgcgaag ctctcgatgc
      901 acctgcgcat cgaatggcgg cacggtctca ccgcgcatcc attttctgga gacaagtgct
      961 ccgctcccgc ctccaccgtt gaactcggca cgctgctcga gaccgcgcgc cggggcgaga
     1021 ttcgcgcgct gcgagaacag ctcgcc
//
LOCUS       DASPLT010001411         1250 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_282471, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001411 DASPLT010000000
VERSION     DASPLT010001411.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1250)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1250)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1250
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_282471"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..230)
                     /locus_tag="VEA63_14380"
     CDS             complement(<1..230)
                     /locus_tag="VEA63_14380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009511777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HYD85248.1"
                     /translation="MRAFTLIELLTVIAIIAILAAILIPTVSSVRVSANKTKTKVQFS
                     QWAGAIEAFRSEYGYAPQVTTDNLLNATAFFAA"
     gene            complement(254..832)
                     /locus_tag="VEA63_14385"
     CDS             complement(254..832)
                     /locus_tag="VEA63_14385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspG"
                     /protein_id="HYD85249.1"
                     /translation="MTESSSSENAASVVRREQMTRAFTLVELLAVLAIVALVAALVLG
                     GGRRATELGQIAKTKAQLAAVSAALEAYKRQHGDYPRTGNAAELLQALIGRRGANGEP
                     TEARGLIDLAQFSTDGEADPFVETRAKLVDAWGAAYVYAYKTGAATAWKMTGFVLYSA
                     GPDREHVAAHPGTGYIDAAAAENADNLYAHAP"
BASE COUNT          220 a          471 c          332 g          227 t
ORIGIN      
        1 gccgcgaaaa acgccgtcgc gttcagcaga ttgtccgtcg tcacctgcgg agcgtagccg
       61 tattcgctgc gaaacgcctc gatcgcgccg gcccattggc tgaattgcac cttcgtcttt
      121 gtcttgttcg cagacacgcg caccgagctc accgtcggaa tcaagatcgc cgccaggatc
      181 gcaataatcg cgatgaccgt gagcagctcg atcaacgtaa aggcacgcat cccgcgccat
      241 cgaccgctcg ttctcatggc gcgtgagcgt aaaggttgtc cgcattctca gccgccgccg
      301 catcgatgta tccagtgccc ggatgcgccg ccacgtgctc gcgatcgggg cccgccgaat
      361 atagcacaaa gcccgtcatt ttccacgccg tagccgcccc cgttttgtaa gcgtagacat
      421 aagccgcccc ccacgcgtcc accagtttcg ccctcgtctc gacgaacggg tccgcttcgc
      481 cgtcggtgct gaactgcgct agatcgatca acccgcgcgc ctcggtcggc tcgccgtttg
      541 ctccgcgccg tccgatcaac gcttgcagca gttccgccgc attacccgtc cgcggataat
      601 ctccatgctg tcgtttatac gcttcaagcg cggccgacac cgccgcgagt tgcgcctttg
      661 ttttcgcgat ctggcccagc tccgtggcgc gacgtccgcc acccaacacc agcgccgcga
      721 ccaaagcgac gatcgcgagc accgcgagca actccaccaa cgtgaacgcg cgcgtcatct
      781 gttcgcgacg cacgacggac gccgcatttt ccgaggagct ggactcggtc atcagccgat
      841 caagcagcca gtcacttgcg tcggcaaatg caaagtaaca gcagacgagt cgttcgcctc
      901 tcgcgtccgc ccgactgctc aaccgtcagc caacgcccgc gcgccgcggc cgtcacacgc
      961 cacgcatttc ctggtcacct tgatccgacc gccttcgcat cgccggcacg ccgtccggcc
     1021 cgatgcgcag tggggacatg gttccttcgt cacgctgcct ttgccctttc ccgtcccctc
     1081 gcacgccgaa cacgacactg accgtccttc ctgcaccaac ccgccccgcc cgccgcacgt
     1141 cgcgcaggga ggcggacccg ttcgcaggct gaaaccgcgt ccgtcgcaca cgctgcataa
     1201 aatcgtgcct ttgcccccgc aggtcgcgca agaaaccgat gtgtgcacgt
//
LOCUS       DASPLT010001412         1069 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_304143, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001412 DASPLT010000000
VERSION     DASPLT010001412.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1069)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1069)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1069
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_304143"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..789
                     /locus_tag="VEA63_14390"
     CDS             <1..789
                     /locus_tag="VEA63_14390"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HYD85250.1"
                     /translation="EVPEFELLRAESELEGARAEFAEAQRVLGEAEQSFRRLLQLPDA
                     RSELILEGRFAPRPFELPLEEAIARALANRPDLQSAGLAVEAARRNQQSLLGNYLPKV
                     EVFASYGARTSYYNSGTELDGWTIGALGQWSIFDGGAARGRRMVARAERRTAETRLTE
                     LEHQIHSRLNELYQGLAQAKVSMEAQQKSVSISARASHDARRLYEVGQADLEQVLQAG
                     MTHRRAESRFSEAVFNYNSIIADIEFAVGGELGDSLSLADTWKR"
     gene            777..>1069
                     /locus_tag="VEA63_14395"
     CDS             777..>1069
                     /locus_tag="VEA63_14395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD85251.1"
                     /translation="MEAIMPAAEAPAASPPSAKPDMRRMEAVIPAALRADVIIRRQFF
                     KHQEYYVIKDPLALTYFRLLPEEAYILTQLDGKRTLRQIAQRFTQRYPNHSRSV"
BASE COUNT          206 a          302 c          360 g          201 t
ORIGIN      
        1 gaggtgcccg agttcgagtt gttgcgcgcc gagtccgagc tggagggtgc gcgcgcggag
       61 ttcgcggaag cgcagcgagt attgggcgaa gcggagcaat cgtttaggcg actgctgcaa
      121 ctgccggatg cgcggagcga gttgattctt gaagggcgtt tcgcgccgcg gccctttgag
      181 cttcctctcg aggaggcgat cgcacgggcg ctggcgaacc gtcccgacct tcagtcggcc
      241 ggcctcgccg tcgaagcagc gcgtcgcaat cagcaatcgc tgctgggtaa ctacctgccg
      301 aaagtggagg tgtttgcgtc gtatggtgca cgcacgtcct actataattc cggcacagaa
      361 ctcgacggct ggacgattgg cgcgctcgga caatggagca tttttgatgg cggcgcggcg
      421 cgaggtcgtc gcatggtggc gcgcgcggag cggcgcacgg ccgagacgcg actgacggag
      481 ctggaacacc aaatccactc gcggttgaac gaactttatc aaggcttggc gcaagcgaaa
      541 gtgtccatgg aagcgcagca aaaaagcgtg agcatctcag cacgggcatc gcacgatgcg
      601 cggaggctct atgaagtcgg gcaggcggat ttggagcagg tgcttcaggc ggggatgacg
      661 catcgccgcg cggagtcgcg tttcagcgaa gcggtattca attacaattc gatcatcgcc
      721 gacatcgagt tcgcggttgg cggagagctc ggtgattcgt taagcttggc cgatacatgg
      781 aagcgataat gccagcggcg gaagcgccgg cggcttcgcc cccttcggca aagccggaca
      841 tgcgcaggat ggaagcggtg attcccgccg cgctgcgcgc cgacgtgatc attcgccggc
      901 agttcttcaa gcatcaggaa tactacgtca tcaaggatcc gctggcgctg acttacttcc
      961 gcttgctgcc cgaggaggcc tatattctca cccagctcga tgggaagcgc accttgcgtc
     1021 agatcgcgca gcgcttcacg cagcgctacc cgaatcattc gcgttcggt
//
LOCUS       DASPLT010001413         1367 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_262400, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001413 DASPLT010000000
VERSION     DASPLT010001413.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1367)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1367)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1367
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_262400"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            26..1294
                     /locus_tag="VEA63_14400"
     CDS             26..1294
                     /locus_tag="VEA63_14400"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HYD85252.1"
                     /translation="MKAPSPSLLPLLALLVGAIVVASTSLPAKPLREETTVWEDRENG
                     IAVHFVYGLVATQSGAILAFSEARIGSGDDETPHHLVLKRSIDSGATWSETIIVERAD
                     GSYWQTQGQPDRRECWANPAALTHRVSGRVFIFYVLNEGAHDGKNLQRATRNFYRYSD
                     DEGKTWSPRVDITDLLNTKADGTPNRDANGEWIRDANGFPCDHLGRAFHMPGPGHGLQ
                     LKDGRLLMQFWNRTAIATDAGPIPRDQRVYGLRVLYSDDGGDEWKAGPAFGEEIGPTE
                     SRLVELDDGSIYLNARVDGAGNAMRRRAIFIGTDRGLTWKLKGFDTEMPSYTNVDSGL
                     LSLRREGRQVLLLSHPRDPRRRVGLTVSVSIDSGNTWVRHEAFNDDGANYSDLVELQD
                     GTIGLLYGNGASAHTGFPVKFIRFNLESIW"
BASE COUNT          265 a          441 c          400 g          261 t
ORIGIN      
        1 ggcgagctct gccgaaacct gctccatgaa agctccgtcc ccttccctgc ttcctctcct
       61 cgctctcctc gttggcgcca ttgtcgttgc ttcaacgtct ctgccggcaa agccgctgcg
      121 cgaggaaaca accgtctggg aggatcgaga aaacggaatc gccgtccact tcgtctatgg
      181 actcgtcgca acacaaagcg gcgcgattct cgcgttctcg gaagcgcgca tcggctcggg
      241 cgatgacgag acgccgcatc acttggtgtt aaagcgcagc atcgattccg gcgctacgtg
      301 gtccgagacg atcatcgtcg agcgcgccga cggcagctat tggcagacgc aaggccagcc
      361 cgaccggcgc gaatgctggg caaacccggc cgcactcacc caccgcgtca gcggacgcgt
      421 ttttattttt tatgtgctga atgagggcgc gcacgacgga aagaacctgc agcgcgccac
      481 ccgcaatttc taccgttaca gtgatgacga gggaaaaacg tggtctccgc gcgtcgacat
      541 cacggacctc cttaacacca aggccgacgg cacgcccaac cgggacgcga acggcgaatg
      601 gattcgcgac gccaatggtt ttccctgcga tcatctcggc cgcgcctttc acatgcccgg
      661 gccgggtcac ggactccagc tgaaggacgg acggctgttg atgcagtttt ggaatcggac
      721 cgccatcgcc accgacgcgg gaccgattcc tcgcgatcag cgcgtctacg gactccgggt
      781 gctttacagc gacgatggcg gcgacgaatg gaaggcaggt cctgcgttcg gcgaagagat
      841 cgggccaacg gagtcgcgcc ttgtcgaatt ggacgacggt tcaatttatc tcaatgcccg
      901 cgtggacggc gccggcaatg cgatgcgccg ccgcgccatt ttcatcggca ccgatcgtgg
      961 cctcacgtgg aaactcaaag gcttcgatac ggagatgccg tcctacacca acgtggattc
     1021 cggcctgctc tcccttcggc gcgaaggacg ccaggttcta ttgctcagcc atccgcgtga
     1081 ccctcgccgc cgcgtgggtc tgacggtgag tgtcagcatc gacagcggaa acacttgggt
     1141 gcgacacgag gcattcaacg acgatggtgc gaactactcg gatcttgtgg agctgcaaga
     1201 cggcacaatc ggcctgctct acggcaacgg cgcctccgca cacaccggct tcccggtgaa
     1261 gttcatccgc ttcaatctcg agtctatctg gtagagcgtc acgacgaccg cgtttcaatt
     1321 tcgctatgcg tctcgacggc gcaacgcgac gcggcgtctc tctctct
//
LOCUS       DASPLT010001414         1022 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_56377, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001414 DASPLT010000000
VERSION     DASPLT010001414.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1022)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1022)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1022
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_56377"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..463
                     /locus_tag="VEA63_14405"
     CDS             <1..463
                     /locus_tag="VEA63_14405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HYD85253.1"
                     /translation="EFALYDQNGEVVQSGRFRGKKIMLNFIFTRCPVATMCPAATVKM
                     IATQKAAREAGVEDIEFVSITLDPAYDTPGVLKDYADTRGIDTANFSLLTGPENAIRD
                     LLANFGVIAEFEGDLLKHTLATLLIDEQGRIIHRADGSQWEPKDFVAKMKR"
     gene            460..>1022
                     /locus_tag="VEA63_14410"
     CDS             460..>1022
                     /locus_tag="VEA63_14410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD85254.1"
                     /translation="MSLANSNAVQAEVGPALTSRQLRQIAIITGVALLVFAMFRWLPT
                     GTNLSHMDFQVDAPNAIDFCDPSNPQFIPVVAVASPVAMTLQTAAAPVTGEPVRATVM
                     LKTASGKPIAAEDLLVVHTRPLHLLVIDPTLGDYQHVHPDPTGVPGEWAFEFTPRGSG
                     TYRVFGDFTPAATNRGLYANADLEVSEA"
BASE COUNT          191 a          270 c          365 g          196 t
ORIGIN      
        1 ggaattcgcg ctttacgatc agaatggcga ggtggtgcaa agcgggcgtt ttcgcgggaa
       61 gaagatcatg ttgaatttca tcttcacgcg ttgccccgtg gcgacgatgt gtccggcggc
      121 gacggtgaag atgatcgcga cgcaaaaagc ggcgcgcgag gcgggcgttg aagacatcga
      181 gttcgtttcg atcacgctcg atccggccta cgatacgccc ggggttttga aggactatgc
      241 cgacacgcgc gggatcgaca cggcgaactt ctcgctgttg accgggccgg agaatgcgat
      301 tcgcgatctg ctcgcgaact tcggcgtgat tgcggagttc gaaggcgact tgttgaagca
      361 cacgctcgcg acgttgttga tcgacgagca gggccgcatc attcaccgcg cggacggcag
      421 ccaatgggag ccgaaggact tcgtggcaaa gatgaagcga tgagcctcgc gaactcaaac
      481 gcggtgcagg cggaggtcgg tcccgctctc accagccgac aactgcggca gatcgcgatc
      541 atcaccggcg tagcgctgct tgtgttcgcg atgtttcgct ggctgccgac ggggacgaat
      601 ctcagccaca tggatttcca ggtggacgcg ccgaacgcga tcgatttctg cgatccgtcg
      661 aatccacagt ttatcccggt ggttgcggtg gcgtcgccgg tggcgatgac gttgcaaacg
      721 gcggcggcgc cggtgacggg cgagccggtg cgggcgacgg tgatgttgaa gacggcgagc
      781 ggtaagccga tcgcggcgga ggatttattg gtggtgcaca cgcggccgct gcacctgctc
      841 gtgatcgatc cgacgctggg cgattatcaa catgtgcatc cggacccgac gggcgtgccg
      901 ggcgagtggg cgttcgaatt tacgccgcga gggagcggca cgtatcgggt ttttggagac
      961 tttacgccgg cggcgacgaa tcgcgggctg tatgcgaatg cggatttgga ggtgagcgag
     1021 gc
//
LOCUS       DASPLT010001415         1408 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_221327, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001415 DASPLT010000000
VERSION     DASPLT010001415.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1408)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1408)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1408
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_221327"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1370)
                     /locus_tag="VEA63_14415"
     CDS             complement(<1..1370)
                     /locus_tag="VEA63_14415"
                     /inference="COORDINATES: protein motif:HMM:NF014877.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucomutase/phosphomannomutase family
                     protein"
                     /protein_id="HYD85255.1"
                     /translation="MSQQIKFGTDGWRGVIADDYTFENVRRVANAIAHYVHKSEDASR
                     GVLVAYDTRFGSRRFAEITAEVLAATGLRVKLASEVTPTPALSLAVKQQGAAGGVMIT
                     SSHNPWSWNGVKYKAAYGGSGSPAIIKQIEGYIDVPPIPAKGGSVEETDFNARYIQTL
                     KAFVNLDLIAKARQKFAIDVMYGAGRGILSGIFKEIGVDHIEIRGEINPLFPGINPEP
                     ILPHVKALQQAVVVHKCHAGLATDGDADRIGAVAEDGSFVDSHKCYAVLLQWLLKRKS
                     WTGVVTRAFNTTRMLDRIAKKYNRELIEHGIGFKYVADVVLSGREVLIGGEESGGIGI
                     PRHLPERDGILNSLLIANVMAEEGKSLGELVEGLQQEFGRHYYGRRDLHLTDEVKQSA
                     LRRSAARPTGIGRFTVQKMEDLDGYKFFLDAPTNGNGAEAWVLVRGSGTEPLLRVYCE
                     AASQELV"
BASE COUNT          240 a          421 c          457 g          290 t
ORIGIN      
        1 acgagttcct gcgaagcagc ttcgcagtag acgcggagca gcggctcggt tccggaaccg
       61 cgcaccagca cccaggcttc ggcgccgttg ccgttagtcg gcgcgtcgag gaagaacttg
      121 tacccgtcga ggtcttccat cttctgcacc gtaaagcgtc cgatgcccgt gggccttgcg
      181 gccgaacggc gcagcgcgct ctgcttcact tcgtcggtga ggtgcaggtc gcggcgtccg
      241 taatagtggc gcccgaactc ctgctgcaaa ccttccacca gttcgccgag cgacttgcct
      301 tcttcggcca tgacgttggc gatgagcagc gagttgagga tgccgtcgcg ctcgggcagg
      361 tggcgcggga tgccgatgcc gcccgactct tcgccgccga tgagcacttc gcgcccgctg
      421 agaacgacat cagccacata tttgaagccg atgccgtgct cgataagctc gcggttgtac
      481 ttcttcgcga tgcggtcgag catgcgcgtg gtgttgaagg cgcgggtgac gacccctgtc
      541 cacgacttgc gcttgagcag ccactggagc agcacggcgt aacacttgtg ggaatcgacg
      601 aagcttccgt cttcggcgac ggcaccgatg cggtcggcgt cgccgtcggt cgcgagtcct
      661 gcgtggcact tgtggacgac aacggcctgt tgtaacgcct tgacgtgcgg cagaatcggc
      721 tcagggttga tgccggggaa cagcgggttg atctcgccgc ggatctcgat gtgatcgacg
      781 ccgatctcct tgaagatgcc ggagaggatg ccgcgtccgg cgccgtacat cacgtcgatg
      841 gcaaacttct ggcgcgcctt ggcgatcagg tcgagattaa cgaaagcctt cagggtctgg
      901 atgtacctgg cgttgaaatc cgtctcctca acgcttccgc ccttggccgg gatcggcggc
      961 acgtcaatgt agccttcgat ctgcttgatg atggccggag agcccgaacc gccgtaagcg
     1021 gccttgtact tcacgccgtt ccagctccac gggttgtggc tggaggtgat catcactccg
     1081 ccggcggctc cctgttgttt gacggcaagc gagagcgcgg gggttggcgt gacttcgctg
     1141 gcaagcttca cgcgcaggcc ggtggccgcc agcacttcgg ccgtaatctc ggcaaagcgg
     1201 cgcgagccga agcgcgtgtc ataggcgacg agcacgcccc tggacgcgtc ttcgctcttg
     1261 tgaacgtagt gggcaatggc gttggctacg cggcggacgt tctcgaacgt gtaatcgtcg
     1321 gcgatgactc cgcgccaacc atcggtgccg aacttgatct gttgagacat gaataaaatt
     1381 tccgaattga gggattttcg aattttcg
//
LOCUS       DASPLT010001416         1243 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_17297, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001416 DASPLT010000000
VERSION     DASPLT010001416.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1243)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1243)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1243
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_17297"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(27..281)
                     /locus_tag="VEA63_14420"
     CDS             complement(27..281)
                     /locus_tag="VEA63_14420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD85256.1"
                     /translation="MKTFVQQKRLILLRADMHRQMLTLEGRHLQQGVGDARNRLAAGR
                     WWLIGGGLVAGWLLTRKLPSLARWLPVMMPAWRLARGLLR"
     gene            complement(278..676)
                     /locus_tag="VEA63_14425"
     CDS             complement(278..676)
                     /locus_tag="VEA63_14425"
                     /inference="COORDINATES: protein motif:HMM:NF018980.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HYD85257.1"
                     /translation="MPMFEDESNSEADGLGASLRRIGASLLGLAQTRIELFSVELQEE
                     KLRLLNLLVWFSVAMALAVAGLLVAIGALGLFLWETAGYAGLVGLVVVTLGGAAGLLW
                     MLRRRILRRPAPFAETAAEFKKDLESLRHQ"
     gene            complement(676..990)
                     /locus_tag="VEA63_14430"
     CDS             complement(676..990)
                     /locus_tag="VEA63_14430"
                     /inference="COORDINATES: protein motif:HMM:NF017742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 family protein"
                     /protein_id="HYD85258.1"
                     /translation="MENAQSKLARERVLADMRTLVADAEDLLKVTANDMGEKAKETRE
                     RLAAALERAKASYSEMQDYGIESAKVAAKKADEAIRSHPYESVGIAFGVGLLLGALLR
                     RK"
BASE COUNT          234 a          418 c          372 g          219 t
ORIGIN      
        1 tcgctgcgcg cgacgtcgat cttcgcctat cgcaacagcc cgcgcgccag acgccacgcc
       61 ggcatcatca ccggcagcca gcgcgccagg cttggcagct tgcgcgtgag caaccaaccg
      121 gccaccagcc cgccgccgat cagccaccaa cggcccgcag cgagccggtt gcgcgcgtcg
      181 cccacgcctt gctgcaagtg ccgcccctcg agagtcaaca tctgccggtg catgtcggcg
      241 cggagcagaa tcagccgctt ttgttggacg aaagttttca ttggtggcgc aggctctcca
      301 aatctttttt gaattccgcc gcggtctccg cgaatggtgc cggccgtcgc agaatgcgac
      361 gacgcagcat ccacaacaaa cccgcggcgc caccgagcgt gaccacgacg agcccgacga
      421 gcccggcata gcccgccgtt tcccagagga acaaaccgag tgcgccgatc gccaccagca
      481 gccctgcgac cgccagcgcc atcgccacgc tgaaccagac gagcaggttc agcaggcgta
      541 gcttttcctc ctgaagctcg acggaaaaca gctcgatgcg cgtttgcgcg agaccaagca
      601 gcgaagcgcc gatgcgccgc agcgaggcac ccaagccgtc ggcttcggag ttggattcgt
      661 cttcgaacat cggcattact tgcgacgcag aagcgcgcca agcagcaatc cgacgccgaa
      721 tgcgatgcca accgattcgt agggatgcga acgaatcgcc tcgtccgcct tcttcgccgc
      781 cactttcgcc gactcgatgc cgtagtcctg catttctgaa tacgaagcct tcgcgcgttc
      841 gagcgcggcg gcgaggcgct cgcgcgtctc cttggctttt tcgcccatgt cgttcgccgt
      901 cactttgagc agatcttcgg cgtccgcgac gagggtgcgc atgtcggcga gcacgcgctc
      961 gcgagccagt ttgctttggg cgttttccat ggtggggaag tgggtttcca tattgttgcg
     1021 gttgattttg gtaggctgat gatctcgaaa aaagaacgga cgctcgcctg aacgacgcgc
     1081 atccattggc gcactacgag gaatcggtcg gccgaaagcc ggcgtcgagt tcgtagggcg
     1141 gcgtcgctcc gtcacgcgac gcgacaaact cgcccattcg gcacgcgctg gcaagtgcca
     1201 gtgcaggatt ggtttcgcgc gcgagcaacg ctccaagcaa acc
//
LOCUS       DASPLT010001417         2443 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_56378, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001417 DASPLT010000000
VERSION     DASPLT010001417.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 2443)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2443)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2443
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_56378"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..388
                     /locus_tag="VEA63_14435"
     CDS             <1..388
                     /locus_tag="VEA63_14435"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HYD85259.1"
                     /translation="AEWNPWGKLDPAMKTTFAGPRAGTGAIMEWSGNNQVGAGRMTVA
                     ESRANERVTYTLDFLKPFPSTCTTEFTFQPQGDRTLVTWAMFGERSFIPKAFGLFMDM
                     DKMLGGFFEQGLADLEANTKPAVANN"
     gene            complement(598..1791)
                     /locus_tag="VEA63_14440"
     CDS             complement(598..1791)
                     /locus_tag="VEA63_14440"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HYD85260.1"
                     /translation="MSTSPLPLSTWAQNVAPSPTLAIDAKAKALQAAGEDVCGFAAGE
                     PDFDTPEHIKEAAIAALKAGKTKYAPTPGIIELRKAIVERYAAEYNFTITPEQVIVSP
                     GGKFNCYLGVLAVCSPGDEVIVPAPYWVSYPEMVKLAGATPKFVLADDKTSFRLTPQM
                     LEAAITPKTKLVILNSPSNPTGAVYSRAELEAIVAVAVKHNLYLLSDEMYEHLVYDGA
                     KPTCVATLSEEAKARTITVAGFSKTYAMTGWRIGTTVAPLPIAKAIAELQSQMSSNVT
                     TFGQYGALAALKEKEKTAAALKTMMAAFDRRRKFLHAELNKIPGITCLLAEGAFYLFP
                     NISSFGLKDVDFCSRLLEAEKVAAVPGSAFGAEGYLRLSYATSDEIIKKGVERLARFC
                     ATLKK"
     gene            complement(2133..>2443)
                     /locus_tag="VEA63_14445"
     CDS             complement(2133..>2443)
                     /locus_tag="VEA63_14445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD85261.1"
                     /translation="LQMDVFWVFHAGRDPVKLLESYSDRWFSLHVKDIRKGAVTNLSS
                     GHAAPTDNVAVGTGQIDWKAVLSTAQKVGVRYYIIEDETPAPLQAIPASLKYLNGLKL
                     "
BASE COUNT          467 a          805 c          713 g          458 t
ORIGIN      
        1 cgctgaatgg aatccatggg gaaagctcga tcccgcgatg aagacgacgt tcgccgggcc
       61 gagggccggc acgggcgcca tcatggagtg gtcgggcaac aatcaagtcg gcgcgggtcg
      121 catgaccgtg gccgagagcc gtgcgaacga gcgcgtcacc tacacgctcg atttcctaaa
      181 accgtttcct tcgacgtgca cgaccgagtt cacgtttcaa ccgcagggcg atcgcacgct
      241 cgtcacgtgg gcgatgttcg gtgaacgaag cttcatcccg aaagccttcg ggttgttcat
      301 ggacatggac aagatgctcg gcggcttctt cgaacagggc ctcgcggact tggaagcgaa
      361 taccaagccg gccgtcgcga acaattgatc ggtcaagctg tagcggcagg cctccgcgcc
      421 tgcctgcttg gattgtcgac ggctcgctcg cgcgccacgc agtttgaatc gtaggttgcg
      481 cgcttgcgcg caacgtggcg cggcgagcgc ccccctgcac ttcgaacgag ccagcaggca
      541 ggcacggagg cctgccgcta ctcgcgaaca gaaaatccaa accgcgcgag ccgcgcctta
      601 cttcttcaac gtcgcgcaga agcgcgcgag ccgctcgacg cccttcttga tgatctcgtc
      661 gctcgtcgca tagctgagcc gcagataacc ttccgcgccg aacgcgctgc ccggcaccgc
      721 agccaccttc tccgcctcga gcaagcgcga gcagaaatcg acgtccttca acccgaagct
      781 cgagatgttg gggaaaagat aaaacgcgcc ttcggccagc aggcaggtga tgccaggaat
      841 tttgttcaac tcggcgtgca ggaacttccg gcgccgatcg aacgccgcca tcatcgtctt
      901 caacgccgca gccgtctttt ccttttcctt caacgccgcc agcgcgccgt attggccaaa
      961 cgtcgtcacg ttcgacgaca tctggctctg caattcggcg atcgctttcg caatcggcag
     1021 cggagccacc gttgtgccga ttcgccagcc cgtcatcgca tacgtcttcg aaaaacccgc
     1081 gaccgtgatg gtgcgcgctt ttgcttcctc gctcaacgtc gcgacgcacg tcggcttcgc
     1141 accgtcgtaa acgaggtgct catacatctc gtcggagagc aggtagagat tgtgtttcac
     1201 cgcgacggct acgatcgcct ccagctccgc acggctgtaa accgcgcccg tcggattcga
     1261 gggcgaattg agaatcacga gcttcgtctt cggcgtgatc gccgcctcga gcatctgcgg
     1321 cgtgagacgg aaactggttt tgtcgtcggc gagcacgaac ttcggcgtcg caccggcgag
     1381 cttcaccatc tcgggataac tcacccaata cggcgcgggc acgatcactt cgtcgcccgg
     1441 cgaacacacc gcgagcacgc cgaggtagca gttgaacttg ccgcccggcg agacgatcac
     1501 ctgctccggc gtgatcgtga aattatactc ggccgcgtag cgttcgacga tcgccttgcg
     1561 cagttcaatg atgccgggcg taggcgcgta tttcgtcttg ccggccttca acgccgcgat
     1621 cgcggcttcc ttgatgtgtt ccggcgtgtc gaagtccggc tcgcccgcgg cgaatccgca
     1681 cacgtcttcg ccggcggctt gcagcgcttt agccttggcg tcgatggcga gggtcggcga
     1741 aggcgcgacg ttttgagccc aggtggacaa cggcagaggc gaggtggaca tgtgagtaaa
     1801 ggcgctcact aaaacgaaac gcggcccgcc aatgcaagcc cgcgaaccgc acgcccgcgt
     1861 ccgctccaac gaaaagagcg ggccgatcgg cccgctcaac tgtgggaggg gctttacgcc
     1921 ccgactcctc gccgcggcgc ggctaaccga agcgatccca gcaagatcag ttcagctgcc
     1981 cgcgaatcac gccaatggtc gcgaatagga atgaagagaa atgatccttc acccaccgtg
     2041 acctggcgcc gaagcacggc tggatttaat tcctcctcgg aaattcgcgt cggttcgcgt
     2101 tgttcgcggg aggtcttctg ccgcgatacg gcttacagct ttagcccgtt caaatacttc
     2161 aagctcgccg ggatcgcctg caacggcgcc ggcgtctcgt cctcgatgat gtaatacctc
     2221 acgccgacct tctgcgccgt gctcagcacc gccttccaat cgatctggcc cgtgcccacc
     2281 gccacgttgt ccgtcggcgc cgcatgaccg ctcgaaagat tcgtcacggc acccttgcga
     2341 atatctttca cgtgcagcga aaaccagcgg tcgctgtagc tctccagcag cttcaccggg
     2401 tcgcgccccg cgtgaaacac ccagaacacg tccatctgca gct
//
LOCUS       DASPLT010001418         1445 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_282562, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001418 DASPLT010000000
VERSION     DASPLT010001418.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1445)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1445)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1445
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_282562"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1035
                     /locus_tag="VEA63_14450"
     CDS             <1..1035
                     /locus_tag="VEA63_14450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HYD85262.1"
                     /translation="YAALCAIVQSDIKKILAYSTLSQLGYMVAAFGLGSMVVQHETDA
                     TAHTMLVATGVGAAMFHLTTHAFFKALMFLGSGSVIHGCHHEQDIFKMGGLRKKMPLT
                     FWTFTIGVLAIIGMPLLAGFFSKDAILFLAYEKNTAVFAILAFVAILTALYMVRLWKI
                     TFLGAPRSHEAEHAHEGGPTLWLPLIVLAALSVAGGYTAFYPQVFTGVFDLIPEAHGS
                     AHTIILITSLAVMTIGALAALFFYKPAATDSLEQKSPGAFNGLLALRNSFDAAYDYYV
                     AKVQQRFAMLLNFLEQIFLAGLIIRGFAGVVGLFGLGARALHVGKLNVYVYWFLGGAV
                     ALWLFATGVL"
     gene            1154..>1445
                     /locus_tag="VEA63_14455"
     CDS             1154..>1445
                     /locus_tag="VEA63_14455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HYD85263.1"
                     /translation="MSNLPFDPLLVSIAAPLALALAIACGLPKRWSVTLAYVAFAIPA
                     LLSLHVWWHFAAATQESGYAFLRTYSTGLEAYGISLKLGLNGIALPLFVLAGL"
BASE COUNT          223 a          512 c          387 g          323 t
ORIGIN      
        1 tacgccgcgc tctgcgcgat cgtgcagagc gacatcaaga agatcctcgc ttactccacg
       61 ctctcgcagc tcggctacat ggtcgcggcg tttgggcttg gttcgatggt tgtccaacat
      121 gaaaccgacg cgactgcaca cacgatgctc gtcgccaccg gcgtcggcgc tgcgatgttc
      181 catctcacca cgcacgcctt tttcaaggcg ctcatgttcc tcggctccgg ctccgtcatc
      241 cacggctgcc accacgaaca ggacatcttc aagatgggcg gtctgagaaa gaagatgccg
      301 ctcacgttct ggacgttcac gatcggcgtg ctcgcgatca tcggcatgcc gctcctcgcc
      361 ggcttctttt cgaaggacgc gattcttttt ctcgcctacg aaaagaacac cgcggtgttc
      421 gcgatcctcg cgttcgtcgc gatcctcacc gcgctttata tggtgcgtct ctggaaaatc
      481 actttcctcg gcgcgccgcg cagccacgaa gccgagcacg cgcacgaggg cggccccacg
      541 ctttggttgc cgctcatcgt tctcgccgcg ctctcggtcg ccggcggtta cactgcattt
      601 tatccgcagg tcttcaccgg cgtcttcgat cttattcccg aagcccacgg cagcgcccac
      661 acgatcattc ttatcacgag cctcgcagtc atgacgattg gcgcgctcgc cgcgctcttc
      721 ttctacaagc ccgccgccac cgactcgctc gaacagaaat cacccggcgc cttcaacggc
      781 ctgctcgccc tgcgcaacag cttcgacgcc gcttacgatt actacgtcgc gaaagtgcag
      841 cagcgctttg cgatgctgtt gaacttcctc gagcagatct tcctcgcggg cctgatcatt
      901 cgcggcttcg ccggagtcgt cggcctgttc ggcctcggcg cgcgcgcgct gcacgtcggc
      961 aagctcaacg tttacgtcta ctggttcctc ggcggcgccg tcgccctgtg gctgttcgca
     1021 acgggagttc tctgatgatg agccgcgctc catattggcc gcaaaaaggc gcaaagatgc
     1081 gcaaaaacca gcccagcctt tttgcgcctt cttgcgcctc tttgcggcca ttccggtcgg
     1141 cctgtctttc gcgatgagca atctgccctt cgatcctctc ctcgtctcga tcgctgcgcc
     1201 gctcgcgctg gccctcgcca tcgcgtgcgg gctgccgaaa cgctggtcgg tcacgctcgc
     1261 ttacgtcgcc ttcgcgattc ccgcgctgct ctcgctccac gtctggtggc atttcgccgc
     1321 ggcgactcag gaaagtggat acgcgtttct ccgcacctat tcgaccggcc tcgaagccta
     1381 tggcatcagc ctgaaactgg ggctcaacgg catcgcgctc ccgctgttcg tcctcgccgg
     1441 cctcg
//
LOCUS       DASPLT010001419         1989 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_262493, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001419 DASPLT010000000
VERSION     DASPLT010001419.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1989)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1989)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1989
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_262493"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..538
                     /locus_tag="VEA63_14460"
     CDS             <1..538
                     /locus_tag="VEA63_14460"
                     /inference="COORDINATES: protein motif:HMM:NF014324.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004620 - phospholipase activity [Evidence
                     IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phospholipase A"
                     /protein_id="HYD85264.1"
                     /translation="ASSSPFYDTSYLPEIAISTDASHPGGSASWFTWMGWRAGYQHES
                     NGRDDADSRSLNRVYLRPRFILGSLDAWAFVMLPEIHAYIGGVEDNSGIKDYRGYGSL
                     RFYFGRNGGPALMVTGWTGRDFDHASYQLDLTVPVRLRRLNVESFLLVQYFNGYGESL
                     RSYDLKTDALRVGFGLVR"
     gene            561..1979
                     /locus_tag="VEA63_14465"
     CDS             561..1979
                     /locus_tag="VEA63_14465"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HYD85265.1"
                     /translation="MTDFRARVTQWRFAMLPACAVALLASGCVHAPLNQPLTRYDPTG
                     GFRYTPRAATAESDISVLLFFSGGGKRAATLSYGVLRELAATSVPAHDGGTRRLLDEV
                     EIVSTVSGGSFTGAYYALHHDGIFVDFERRFLKRNVNRALLRRVATPTHWRNLASPYY
                     GRSDLAADYYDRTVFDGATFGDLVRKGGRPLLLINATDMANGEQFTFSQIRFDLLGSD
                     LGEFPLSRAVAASSAAPLLLTPITLQNHAGAAGEVQSAFLPDGPADPALSPREREVRE
                     LMLSYTDAKERPFIHLVDGGLSDNLGLRSIMDASLLSGGLGELSERIGMPPANKILVI
                     IVNAATRRGSEWSKREAVPGLRHSIEQLGDNIGERVNHRSLELFRQMLDDWRRKARER
                     AAATPRYATGWHPPLPDYYLVTVVFEDIPHRQERYFFQNLPTSFHLPDETVDRLTDVG
                     GRLLRESGEFQRLLGDIGATAR"
BASE COUNT          319 a          631 c          644 g          395 t
ORIGIN      
        1 cgcgagctcc agtccgtttt acgatacgag ttatctgccg gagatcgcca tcagcacgga
       61 cgcctcgcac ccgggcggga gcgcgtcgtg gttcacgtgg atgggctggc gcgccggcta
      121 ccaacatgaa tcgaacggcc gcgacgacgc cgactcgcgc agcctcaacc gcgtgtattt
      181 gcgtccccgt ttcatcctcg gctcactgga tgcgtgggcc ttcgtgatgc tgccggagat
      241 ccacgcctac atcggtggcg tggaagacaa ttccgggatc aaggactatc gcggctacgg
      301 aagcctgcgt ttctacttcg gaaggaacgg cggcccggcg ctcatggtga cgggatggac
      361 cgggcgcgac ttcgaccatg cgagttatca actcgatctg acggtgcccg tccgccttcg
      421 tcggctgaac gtggagtcgt tcctgctggt gcagtatttc aacggctatg gtgaaagtct
      481 gcgctcctac gatctcaaaa cggatgccct ccgcgtcggc ttcgggctcg tgcgctgacg
      541 tccaccgttc tcacgggccg atgaccgact tccgtgcgcg cgtgacgcag tggagattcg
      601 cgatgctgcc agcatgcgcg gtcgccctgc tcgcttccgg ttgcgtgcat gcgccgctca
      661 accagccgct gacgcgctac gatcccacgg gaggttttcg ctacacgccg cgagcggcga
      721 cggcggagtc ggatatttcg gtgctgctgt ttttctccgg cggcggcaaa cgcgccgcca
      781 cgctgtctta cggcgtgctg cgcgagctgg cggcgacgtc cgtgccggcg cacgacggcg
      841 gcacacggcg tttgctcgat gaggtcgaaa tcgtttcgac cgtttcaggc ggcagtttca
      901 cgggggctta ctatgcgctg caccacgacg gaatcttcgt cgatttcgag cggcgttttc
      961 tgaagcgaaa cgtcaaccgc gcgttgctgc ggcgcgttgc gacgccgacg cattggagaa
     1021 atctcgcttc gccttactac gggcggagcg acctcgcggc ggattactac gatcgaaccg
     1081 tgttcgacgg cgcgaccttc ggcgatctcg tgcgcaaggg tggccgtccg ttgctgctga
     1141 tcaacgcgac cgacatggcg aacggcgaac aattcacgtt cagccagatt cgcttcgacc
     1201 tcctcggctc ggatctcggt gaatttccgc tctcccgcgc cgtcgccgcc tcctccgcgg
     1261 caccgctgct gctgactccg attacgctgc agaatcacgc gggcgcggcg ggcgaggtgc
     1321 agtccgcttt tctgcccgac ggtccggcgg atccggcgtt gtcgccgcgc gaacgcgaag
     1381 tgcgcgagtt gatgctgtcc tacaccgatg cgaaggagcg cccgttcatc catctcgtcg
     1441 acggcggtct ctccgacaat ctcgggctgc gcagcatcat ggatgcaagc ctgctgtcgg
     1501 gtgggctggg ggagttgtcc gagcgcatcg gcatgcctcc ggcgaacaag atcctcgtga
     1561 tcatcgtgaa cgccgcgacc cggcgcggca gcgagtggag caagcgcgaa gcggtgccgg
     1621 gactgcgcca ttccatcgag cagctcggcg acaatatcgg cgagcgggtg aatcatcgtt
     1681 cgctcgaact ctttcgacag atgctcgacg actggcgtcg aaaggcgcgc gaacgcgccg
     1741 cggctacacc gcgttatgcc acgggctggc acccgccgct gccggactac tatctcgtga
     1801 cggtcgtctt cgaagatatc ccgcaccggc aggagcgtta ttttttccag aacctgccga
     1861 cgagttttca tttgccggat gaaacggttg accggttgac cgacgtcggc gggcggctcc
     1921 tccgcgaatc gggcgagttt cagcggctgc tgggtgatat tggcgcgacc gcgcgatgat
     1981 tcacgcggg
//
LOCUS       DASPLT010001420         1063 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_241179, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001420 DASPLT010000000
VERSION     DASPLT010001420.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1063)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1063)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1063
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_241179"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..259
                     /locus_tag="VEA63_14470"
     CDS             <1..259
                     /locus_tag="VEA63_14470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007361275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD85266.1"
                     /translation="YKQLPKKGDVYTIREVFLGREKIVRGGDSATVGLLLEELKNPPD
                     PFHQGKQELGFSSERFAPLEELPAEEAEAEEVVHVGAWAPA"
     gene            384..800
                     /locus_tag="VEA63_14475"
     CDS             384..800
                     /locus_tag="VEA63_14475"
                     /inference="COORDINATES: protein motif:HMM:NF019590.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NIPSNAP family protein"
                     /protein_id="HYD85267.1"
                     /translation="MIRSLRLLLIFTFAFGASGFVSAATAETRVFELRTYTATPGKLD
                     ALLARFRNHTCALFTKHGMTNIGYWLPTDDKDGASRKLVYLLAHESRDAAKASWAAFI
                     ADPEWKAVHAASEANGSLVEKIDSVYLNPADFSAIK"
BASE COUNT          217 a          366 c          284 g          196 t
ORIGIN      
        1 ctacaagcag cttccgaaga agggcgacgt gtataccatc cgcgaggttt tcctcgggcg
       61 ggaaaaaatc gtgcgcggcg gcgacagcgc cacggtcggg ctcttgctcg aagagctaaa
      121 aaatccgccc gatccattcc atcaagggaa gcaggagctt ggtttttcct cggaacgttt
      181 tgccccgctc gaagagcttc ccgccgaaga agccgaagcc gaggaagtcg tccacgtcgg
      241 cgcttgggcc cccgcctgaa accattgact tgcgaccgtc agatggcgcg ttcgccaaac
      301 ctaatcgcgc gacccggcat catccaccat cgggtcgttc accgattcga tcggagcttc
      361 gcccctgtcg gaaaaaacat tttatgatcc gctccctccg tctcctcctc atcttcacgt
      421 tcgccttcgg cgcgtccggt tttgtctcgg cggccacggc cgagacacgc gttttcgagt
      481 tacgcactta taccgccacc cccggcaaac tcgacgcgtt gctcgcgcgc ttccgcaacc
      541 acacgtgcgc acttttcacg aagcacggca tgaccaatat cggttattgg ctgccgaccg
      601 acgacaaaga cggcgccagc cgcaagctag tctatctgct cgcgcacgag agccgggatg
      661 cggcgaaagc ttcgtgggcg gccttcatcg ccgatcccga gtggaaagcc gtccacgccg
      721 ccagcgaggc caacggctcg ctcgtcgaga aaatcgactc ggtttaccta aacccggctg
      781 acttttccgc gatcaagtaa ttgaaggtcg ctccgggtgg cgcagcgaag ctcgctgcgc
      841 agattctgcg gccgagcaaa aacccgacca ccgtcgcgag cgcgattccg atcgcaacgc
      901 cgatcgccac attcccaaaa gcgctgccga ttacagcgcc cacgccgaca ccgatcgcga
      961 cccacagcgc gagcttcaca tctttctgca taaaatctta ccggccgcgc cgagggcgat
     1021 ctccgttgct cccggcgcga ccggattcca acccgactca tcg
//
LOCUS       DASPLT010001421         2679 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_262565, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001421 DASPLT010000000
VERSION     DASPLT010001421.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 2679)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2679)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2679
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_262565"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..448
                     /locus_tag="VEA63_14480"
     CDS             <1..448
                     /locus_tag="VEA63_14480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TRCF domain-containing protein"
                     /protein_id="HYD85268.1"
                     /translation="RHEDSYTAIRDAENDELGGEIAKIQARLPSTYIPETRLRIDFYR
                     KLALAETPAKLKEIEADLRDRFGKFGDEVRALLLVTEIRVRAEQKNILSVETETNRLK
                     CLRNSGRRDDWVQVGTRFPRLTAPKPLLRLREIISFLNNLPTPPSP"
     gene            445..1440
                     /locus_tag="VEA63_14485"
     CDS             445..1440
                     /locus_tag="VEA63_14485"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HYD85269.1"
                     /translation="MMPGRLRLAFLFIGLASTALAQSSEPVDDGLNLRFANGIAAIAE
                     DKVITVDDVRREISPLVAQLQRDARSEREFNEKLEQLQDEVIQSLIDRVLIVKEFRKD
                     EKKHIPPSFIENRIADIQSEQFDNDRSKFLAYLHARGMTTREYRREVEEDIIYNYMRQ
                     QQRKSQSVVSPVRIETFYRENKDRFYQEDGVHLRLIQFTRNSGESDAVLQEKANLVFA
                     RLKAGEKFEDLAREYSQDSRRAKGGDWGWQKRSDLKPEFSEPLFALKPGEASDAILLP
                     EGCFILYAEDRKYAGIQPIDSVRDEIERVLLQQMTRNSQEHWLERLRRNGYVKHY"
     gene            1707..2198
                     /locus_tag="VEA63_14490"
     CDS             1707..2198
                     /locus_tag="VEA63_14490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD85270.1"
                     /translation="MSTLEWLEALSYIVTIVGLPFAIVVFIKEQRKERQNDDEELYLQ
                     LSDEYSKFLRLVLDHSDLHLMTRAEPDAPFTSEQIERRNVLFEVLIAIFERAYILVYE
                     EHMSRQATRLWQTWADYMRDWCRRPDFRELLPELLQGEDPEFQAYIRKISAEESTRAN
                     QAA"
     gene            complement(2355..>2679)
                     /locus_tag="VEA63_14495"
     CDS             complement(2355..>2679)
                     /locus_tag="VEA63_14495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ThuA domain-containing protein"
                     /protein_id="HYD85271.1"
                     /translation="IARGIDRCLDLPAEEMYGEPFGIPPPDEQVFISWFEGGEVFRSG
                     CCWTRGNGKIFYFRPGHETYPTYHDANVRRVIANAIEWARPQGKWVDVPTNVKEPREA
                     IRAKG"
BASE COUNT          584 a          936 c          685 g          474 t
ORIGIN      
        1 tcgacacgaa gacagctaca ccgccatccg cgacgccgag aacgacgaac tcggcggcga
       61 gatcgcgaaa attcaggcgc gtttgccatc cacttacatt ccggagaccc ggctccgcat
      121 cgacttctac cgcaagctgg cgctcgccga gacgcccgcg aagctgaagg agatcgaagc
      181 cgacttgcgc gaccgcttcg gcaaattcgg cgacgaggtg cgcgcgctgt tactcgtcac
      241 cgagatacgc gttcgagcgg aacaaaaaaa catcctttcc gtcgaaacgg aaacgaaccg
      301 gttgaagtgt ctgcggaaca gcggccgccg cgacgactgg gttcaagttg gaacccggtt
      361 tccaaggttg accgccccaa agcccctcct acggttgcgc gaaattattt cgtttctgaa
      421 caatctgccc acgccccctt ccccatgatg ccaggtcgcc tccgtctcgc gtttctcttc
      481 attggcctcg cctccaccgc tctcgcccag tcctccgagc ccgtcgacga tggcctgaac
      541 ctgcgtttcg ccaacggcat cgccgcgatc gccgaggaca aggtcatcac cgtcgacgac
      601 gtccgccgcg aaatctcccc cctcgttgcc cagctccagc gcgacgcccg cagcgaacgc
      661 gagttcaacg agaagctcga gcaattgcag gacgaggtta tccaaagcct catcgaccgc
      721 gtgctgatcg tgaaggaatt ccgcaaggac gagaagaagc acatcccgcc gagcttcatc
      781 gaaaaccgca tcgccgacat ccagagcgag caattcgaca acgaccgctc caagttcctc
      841 gcttacctcc acgcccgcgg catgaccacg cgcgaatatc gccgcgaagt cgaagaggac
      901 atcatctaca actatatgcg ccagcagcag cgcaagtccc agagcgtcgt cagccccgtg
      961 cgcatcgaga cgttttaccg cgagaacaaa gatcggtttt accaggagga cggcgttcac
     1021 ctccgcttga tccaatttac ccgcaacagc ggcgagtccg acgcggtgct tcaggaaaaa
     1081 gccaatctcg tcttcgcccg cctgaaggcc ggcgaaaaat tcgaggatct cgcccgcgaa
     1141 tacagccagg attcgcgtcg tgccaaaggc ggcgattggg gctggcaaaa acgctccgac
     1201 ctgaagcccg agttcagcga gccgctcttc gcgctcaaac ccggcgaagc cagcgacgcg
     1261 attttgctcc ccgaaggctg cttcattctc tacgccgaag accgcaagta tgccggcatc
     1321 cagccgattg acagcgtccg cgacgagatc gagcgcgtgc tgctccagca gatgacgcgc
     1381 aacagccagg aacactggct cgaacgcctc cgccgcaacg gctacgtgaa gcattattga
     1441 gccgcgcccg cgccgagcgc gctcgcgaat tcaatcccga aggccggtcc aacacgacca
     1501 gcctttttgt tgcacggacc gcgccgcgcg gaagctccaa gcacgggagg aaagctccaa
     1561 gcgccaacat ccaagctcca atgaaacttc aatttccaac tttcaaaatc ccgccccggt
     1621 aaggattgga gcttgaggtt tggagcttca ctggagcttg gatgttggag cttggagctt
     1681 ccgccgttgc ggcgcatcct gcgtccatgt ccacgctcga atggctcgaa gcgctcagct
     1741 acatcgtgac catcgtcggt ctgccgtttg cgatcgtcgt tttcatcaaa gagcagcgca
     1801 aagaacgaca gaacgacgac gaggagctct acctccagct ctccgatgaa tactcgaaat
     1861 tcctccgcct cgtcctcgat cactccgact tgcatctgat gacgcgcgcc gagcctgacg
     1921 cacctttcac ctccgagcaa atcgagcgcc gcaacgtcct gttcgaagtc ctcatcgcga
     1981 tcttcgagcg cgcctatatc ctcgtttacg aggagcacat gagccgacag gccactcgcc
     2041 tctggcaaac ctgggccgac tacatgcgcg actggtgccg ccgccccgac ttccgcgagc
     2101 tcctgcccga actcctccaa ggcgaagacc ccgagttcca ggcctacatc cgaaaaatct
     2161 ccgccgaaga gtccactcga gcgaaccagg ccgcgtagtc cttaaaaaat ctttctcttt
     2221 ctcgttctct ttctctttct ctcagcccga gacagcgcca gccgtccgac cggagagaac
     2281 gagaacgatc acgagaacga gaatgacacc agccccgcct cgctacgccg ccgagcggcg
     2341 ccccgagcca ccgctcaccc cttcgcacga atcgcctccc gcggctcctt cacattcgtc
     2401 ggcacatcga cccactttcc ctgcggccgc gcccattcga tcgcgttcgc gatcacccgg
     2461 cgcacgttcg cgtcgtgata agtcggatac gtttcgtgtc ccgggcgaaa atagaaaatc
     2521 ttcccgtttc cccgcgtcca gcagcatccg ctccgaaaca cctcgccgcc ctcgaaccac
     2581 gagatgaaca cctgctcgtc cggcggcgga atcccaaacg gctcgccata catttcctcc
     2641 gcaggcagat cgagacaccg atcgattccg cgcgcgatc
//
LOCUS       DASPLT010001422         2368 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_262567, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001422 DASPLT010000000
VERSION     DASPLT010001422.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 2368)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2368)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2368
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_262567"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..883)
                     /gene="aroC"
                     /locus_tag="VEA63_14500"
     CDS             complement(<1..883)
                     /gene="aroC"
                     /locus_tag="VEA63_14500"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007361617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HYD85272.1"
                     /translation="MPNSFGTLFRITTWGESHGAAVGVVIDGCPPKLPISVEEIQAEL
                     DRRRPGQSDIVTPRKEEDRVEILSGIFEGRTTGTPISMLVRNADQRPGAYDEMREKFR
                     PSHADFTYQAKFGIRDHRGGGRSSARETIGRVAAGAIAQKILRLAGARSAVGSAGAAG
                     SGNPALQGVEIRAFVTQIHDIVTPVEALEKFPTLAEVEANAVRCPHAETAAKMIERIK
                     AVRSEGDSVGGVIECRVRGVPVGLGEPVFDRLDAELAKAMLSLPATKGFEIGSGFAGT
                     RLKGSEHNDPFESREGAV"
     gene            1163..>2368
                     /locus_tag="VEA63_14505"
     CDS             1163..>2368
                     /locus_tag="VEA63_14505"
                     /inference="COORDINATES: protein motif:HMM:NF015891.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretin N-terminal domain-containing protein"
                     /protein_id="HYD85273.1"
                     /translation="MTLRSLCLVFAVSLAAAVSFAQTPASEAVTAPDELVGPIKLPDA
                     DIDTVLGLLEIYTGRSILRPQQLPTATYRLVIDKKIPKSEAVLALETVLALNQVGVAP
                     LGDQFLKVVALSQVKTEAPEMISGSTFDFPPSGRIATKLFQLNFLRVNEFIPQITPLL
                     SPGVANGVVPLDKSNAALITDSISNLQRVEMLIEELDRPIMQGLTPKFYTLRNGAKAS
                     DLVGKLRAMFQGPLQNQLGSSISYSADDRTNQIILLADERQIPLFDDLIAKLDVRSDP
                     NTRNEVIYLKHASAKEVAPLLSQLVSGQTSAAQKAGGGTRNPNVPNQPPPPPEATAVM
                     ESVEGTNEFSDLVTILADERSNAVVISGTVDDIRLIRDLVDKVDILLAQVRIEVIIAE
                     VTLNNSDRSG"
BASE COUNT          435 a          899 c          604 g          430 t
ORIGIN      
        1 gcaccgcgcc ctcgcgcgac tcgaacggat cgttgtgctc cgagcctttt aaacgcgtgc
       61 cggcaaaacc gctgccgatc tcgaatccct tcgtcgctgg caacgacaac atcgccttcg
      121 ccagttcggc atccagccga tcgaacaccg gctcgcccag ccccaccggc acgccgcgca
      181 cgcgacactc gatcacgccg ccgaccgaat caccctcgct gcgcaccgcc ttgatccgct
      241 cgatcatctt cgcagcggtt tccgcatgcg gacaacgcac cgcattcgcc tccacttccg
      301 ccaacgtggg aaatttctcc aacgcctcca ccggtgtcac gatgtcgtga atctgcgtca
      361 caaacgcccg aatctcgacc ccttgtaggg cggggtttcc cgaccccgcc gctccggctg
      421 accccaccgc gctccgcgcg cccgccagcc gcaaaatctt ttgcgcgatc gcacccgccg
      481 ccacgcgccc gatcgtctcg cgcgcggaac tgcgcccgcc gccgcggtga tcgcgaatcc
      541 caaacttcgc ctgatacgtg aaatccgcgt gcgatggccg gaatttttcc cgcatctcgt
      601 catacgcacc cggccgctgg tcggcatttc tcaccagcat cgaaattggc gtgcccgtcg
      661 tgcggccctc gaaaattccc gagaggattt ccacacggtc ctcctctttg cgcggcgtga
      721 cgatgtcgct ttgccccggc cggcgacggt cgagctcggc ctgaatttcc tccaccgaga
      781 tcggcaattt cggcggacag ccgtcgatca cgacgccaac cgccgcgccg tggctttcgc
      841 cccaagtggt aatcctgaaa agtgttccga agctgttcgg catcgcgacc gcgcagcgtt
      901 ggcatcgcca ctttcccccg caagcggcaa atcctccgcg tagcggcagg cctccgtgcc
      961 tgcccgccgt ccgaccgtcg gtgatggtga acaggccgac gattctccgc ttcgctttcc
     1021 tcgggccttc ccccaaactt ccggcaacct tcgcgtcaac ttgccgtctg agcacatcac
     1081 cgtttacaac cggcgggcat tcgcctctga tttgctcccc cgatccctcg gccgaccacg
     1141 ttcaccgctc cgccttccct gcatgaccct gcgttccctt tgtctcgttt tcgccgtcag
     1201 cctcgccgcc gccgtttctt tcgcccagac gcctgcgtcc gaagcggtga ccgcgcccga
     1261 tgaactcgtc ggcccgatca agctgcccga cgccgacatc gacacggtcc tcggcctgct
     1321 cgagatctac accggtcgga gcatcctgcg tccgcaacaa ctcccaaccg cgacgtatcg
     1381 gctcgtgatc gacaaaaaga tcccgaagtc ggaagcggtc ctcgcgctcg agaccgtgct
     1441 cgcgctgaac caggtcggcg tcgcgcccct gggcgatcag ttcctcaaag tcgttgcgct
     1501 ctcgcaggtg aaaaccgaag cgcccgagat gatctccggc tcgaccttcg attttccgcc
     1561 cagcggccgc atcgcgacga agctgttcca gttgaatttc ctgcgcgtca acgaattcat
     1621 cccgcaaatc accccgctcc tttcccccgg cgtcgccaac ggcgttgtcc ctctcgacaa
     1681 gtccaacgcc gcgctcatca ccgattcgat cagcaatctt caacgcgtgg agatgttgat
     1741 cgaagagctc gaccgcccga tcatgcaggg cctcacgccg aaattctaca cccttcgcaa
     1801 cggcgcgaaa gccagcgatc tcgtcggaaa gctccgcgcg atgttccaag gcccgctgca
     1861 aaaccagctc ggcagttcga tctcctacag cgccgacgac cgcacgaacc aaatcatcct
     1921 cctcgccgac gaacgccaga tcccgctctt cgacgacctg attgcaaagc tcgacgtccg
     1981 ctccgatccc aacacgcgca acgaggtcat ttatctgaag catgcctccg ccaaagaagt
     2041 cgccccgctc ctcagccagc tcgtctccgg ccagaccagc gccgcgcaaa aagccggcgg
     2101 cggcacgcgc aatccgaatg tgcccaacca acctccgccg ccacccgaag ccacggcggt
     2161 catggaatcg gtggaaggca ccaacgagtt tagcgacctc gtcacaatcc tcgccgacga
     2221 acgtagcaat gccgtggtga tttcgggcac ggttgacgac atccgcctga tccgcgacct
     2281 cgtcgacaag gtcgacatcc tcctcgcgca ggtccgcatc gaggtgatca tcgcggaagt
     2341 gacgctgaac aacagtgatc gaagcggc
//
LOCUS       DASPLT010001423         2451 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_56480, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001423 DASPLT010000000
VERSION     DASPLT010001423.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 2451)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2451)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2451
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_56480"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(187..>2451)
                     /locus_tag="VEA63_14510"
     CDS             complement(187..>2451)
                     /locus_tag="VEA63_14510"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HYD85274.1"
                     /translation="DAIAAEFAWPILQRFFEKNLYPQFTTWRKDDGWHVRRGELQLGS
                     ALPGNGKISWAEVHDQIGWTVFLQEAWGDSTRLAKQFYSSQPDRTASPVASAGETAPD
                     WLVIEISDPLAVRSQLRPRVGAVLTAGGAVVASTRSVAGPGTISAVALQAALTTEAGF
                     ELCCACIREAVIGRRFDEPVSLRMRLAERAAQRPLLSPLADVAVLPRRAGPCGSSASR
                     RAALPTAVLPEIQRWHAQPVRTPLPFSTENTAPSRVLYLPDLATGLNTHATARMPSKT
                     SQYDGAFFEDLFHQNSNPWHYDTPYEQTKYDQTLALLPPRRFGRALELACAEGHFTQR
                     LATRVDALLATDISPTALQRAASRCREHANVSFKPLDLVADPLPGTFDLIVCSEVLYF
                     LGTVQNLRHVLAKIARALKPGGCFVTAHAHLVADEPHRPGFDWDLPFGAKKISDVLAN
                     MSQLRLVRDLRTPMYRVQVYERQSPLRRWLPRRAPQRALLSQPTELPPHAASHAQWRG
                     GVPQRLQGPPKVKTERLPILMYHHVSPDSVGHLDRYRVTPRQFEEQLQFLAERGYRSV
                     SLEEWRRASVARTPLPGRCVAITFDDGYRNFLEHAWPLLLRYGFTATMFVPTRDIGTS
                     DRWENELANGTPLLSWDQLRKLRNEGLEIGSHSHVHPMLTSLSPTEVAREALLSRTLI
                     DEHLGTGVTSFAYPYGEYDRVVERVIGGCGYTFALTCEERPAELQDRLLALPRIEVRG
                     DCTRGDFADLLPET"
BASE COUNT          474 a          725 c          817 g          435 t
ORIGIN      
        1 aattcagttc caccacgaat ggacacgaac ccacacgaat gaaatcgagt tcggatgcag
       61 aatcggatcg ggacaccaaa cggtgatgca cacgatcggt ccaaaagctt tcctctgcct
      121 cctctgaatt cttgtccatt cgtgtccatt cgtggtttct caactgcacg aatccggcgt
      181 cgcgctttaa gtttcgggca gcaaatcggc gaagtcgccg cgcgtgcaat cgccgcggac
      241 ttcgatccgc ggcagcgcga ggagccggtc ctgcaattcg gcaggacgtt cctcgcacgt
      301 gagagcgaac gtgtagccgc atccgccgat cacccgctcc acgacgcgat cgtattcgcc
      361 gtagggataa gcgaagctcg tgacgcccgt gccgaggtgt tcgtcgatca atgttcggga
      421 gagcagcgct tcgcgcgcaa cctcagtggg cgacagactg gtcagcatcg gatgcacatg
      481 agagtgcgag ccgatttcaa ggccctcgtt gcggagtttt ctcagctggt cccaagagag
      541 gagcggagtg ccgttcgcca gctcgttttc ccagcgatcg gaggtgccga tgtcgcgcgt
      601 gggcacaaac atcgtcgcgg tgaaaccgta acgcaggagc aacggccagg cgtgttcgag
      661 aaaattgcgg tagccgtcgt cgaacgtgat ggcgacacag cggccgggaa gcggcgtgcg
      721 cgccacgctg gcgcgtcgcc attcctcgag cgaaacgctt cgatagccgc gctcggcgag
      781 aaactggagt tgctcctcga attgccgcgg cgtgacgcga taacggtcaa gatggccgac
      841 gctgtccggg ctgacgtggt gatacatcag gatcggaagc ctttcggttt tcactttcgg
      901 cggaccttgc aaccgctgcg gaacgccgcc gcgccattgc gcatgcgagg cagcgtgagg
      961 cggaagctcc gtgggctggg aaagcaacgc gcgctgcggc gcgcgccgtg gaagccagcg
     1021 acggagcggc gactggcgct cgtaaacctg cacgcgatac atcggcgtgc gcagatcgcg
     1081 caccaatcga agttgtgaca tgttggcgag cacgtcggag attttcttcg caccgaacgg
     1141 cagatcccag tcgaaccccg ggcgatgcgg ttcgtcggcg acgaggtgtg cgtgcgcggt
     1201 gacaaagcag ccgccgggtt tcaacgcccg cgcaattttg gcgaggacgt ggcgcaggtt
     1261 ttgaaccgtg ccaagaaagt agagcacctc gctgcagacg atgagatcga aggtcccggg
     1321 cagcggatcg gcgacgagat ccagcggctt gaagctgaca ttggcgtgct cgcggcaacg
     1381 gctcgccgcc cgctgcaatg ccgtgggcga aatgtcggtg gcgagaagcg cgtcgacacg
     1441 cgtcgccagc cgttgcgtga aatgtccttc cgcgcaggcc agttcgagcg cacgaccgaa
     1501 gcgccgcggc ggcagaagcg cgagcgtctg atcgtatttg gtctgctcgt aaggtgtgtc
     1561 gtagtgccac ggattgctgt tctgatgaaa cagatcctcg aaaaaggctc cgtcgtattg
     1621 ggaggttttc gagggcatgc gagcggttgc gtgcgtgttg agtccggtcg cgaggtctgg
     1681 cagataaagc acacgcgagg gcgccgtgtt ttctgttgaa aatggaagcg gtgtgcgaac
     1741 gggttgcgca tgccatcgct gaatttccgg caacacggcg gtcggcagtg ctgcgcgccg
     1801 cgacgcgctg ctgccgcaag ggccggcgcg tcgcggcaac accgccacat cggccaacgg
     1861 ggaaagaagc gggcgttggg cggcgcgttc ggcgaggcgc atgcgcaaag aaaccggttc
     1921 atcaaaacgg cgtccgatca cggcttcacg gatgcacgcg cagcagagtt cgaaacctgc
     1981 ttccgtcgtg agcgcggctt gcagggcgac agcgctgatt gtgcccggcc cggccacgga
     2041 gcgcgttgaa gccaccaccg caccgcccgc cgtcagcacc gcgccgacac gcggccgaag
     2101 ctgcgagcga accgccaacg ggtcgctgat ctcgatgacg agccagtcgg gtgcggtttc
     2161 gcctgcgctc gccaccggcg atgcagtgcg atcgggctgc gatgaataaa attgtttcgc
     2221 gagccgcgtg ctgtcgcccc acgcctcctg caaaaacacg gtccaaccga tttgatcgtg
     2281 cacctcggcc caggagattt tgccgttgcc cgggagcgcc gatccgagct ggagttcgcc
     2341 gcgacgcacg tgccagccgt cgtctttgcg ccacgtggtg aactgaggat aaagattttt
     2401 ctcgaagaag cgttgcagga tcggccaggc aaattcggcg gcgatggcat c
//
LOCUS       DASPLT010001424         1295 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_97810, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001424 DASPLT010000000
VERSION     DASPLT010001424.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1295)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1295)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1295
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_97810"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            143..1063
                     /locus_tag="VEA63_14515"
     CDS             143..1063
                     /locus_tag="VEA63_14515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase"
                     /protein_id="HYD85275.1"
                     /translation="METVVFQGHALTRWRVGSSTFLAFPERGARLMNWSLALADGSVR
                     DVVYWPEDADFSKFARVRGGNPILFPFVGRTFDAGDIYHWRAADGIRRPMPMHGLARQ
                     GQFKTLWSDPRGFAAQFVPDDEARAIYPYDYEFVVTYRFEPLGLVCEFSLKNLGETPL
                     PWCAGHHFYFTLPWSDNRTRADYTLQLPAAERFQQNPAGQLIAGPQLKLEEPLSNAAL
                     IDTLHTKLRSNEALVREGARGGTLRLQLGTDKVPPPDATFVTWSADETTPYYCIEPWM
                     GPPNAPEHRRGLHLVPPLETGTFTVSVSIK"
     gene            1123..>1295
                     /gene="tatA"
                     /locus_tag="VEA63_14520"
     CDS             1123..>1295
                     /gene="tatA"
                     /locus_tag="VEA63_14520"
                     /inference="COORDINATES: protein motif:HMM:NF014470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HYD85276.1"
                     /translation="MTPELFFFAILPDLGGGEMMLILLVMLLLFGGDKMPQMAKSLAK
                     TLREFKRAASEVER"
BASE COUNT          237 a          464 c          347 g          247 t
ORIGIN      
        1 gaagctgggg cagccatcca cgcatttccc agcagtgaat acctggcagc gccgacagag
       61 caagccgtcg ctccctcgaa aacccctacg cctttccata aaacgcttgg cgcgatgcac
      121 gcgccgggaa atcgtgctcc cgatggaaac cgtcgtcttt caaggccacg ccctcacgcg
      181 ctggcgcgtg ggcagttcga ccttcctcgc gttccccgaa cgcggcgcgc ggctcatgaa
      241 ctggagcctc gctctcgccg acggctccgt gcgcgacgtc gtctactggc ccgaggacgc
      301 cgacttttcc aaattcgcgc gcgtgcgcgg cggcaatccc atcctctttc cttttgtcgg
      361 acgcacgttc gacgccggcg atatttacca ctggcgcgcc gccgatggca ttcgccggcc
      421 gatgccgatg cacggcctcg cacgccaagg ccagttcaag actctctgga gcgacccgcg
      481 cggtttcgcc gcgcaattcg tgcccgacga cgaagcccgc gccatctatc cctacgatta
      541 cgaattcgtc gtcacctatc gcttcgagcc gctgggcctc gtgtgcgagt tctccctcaa
      601 aaatctcggc gagaccccgc tgccgtggtg cgccggacac catttttatt tcaccctgcc
      661 ctggagcgac aatcgcacgc gcgccgatta cacgcttcaa ctccccgcgg ctgagcgttt
      721 ccagcaaaac cccgccggcc agctcatcgc cggcccgcaa ctaaaactcg aggagccgct
      781 gtccaacgcc gccctcatcg ataccttgca cacgaagctc cgcagcaacg aagccctcgt
      841 gcgcgagggc gcgcgcggcg gcacgctgcg ccttcagctc ggcaccgata aagtgccgcc
      901 acccgacgcc acgttcgtca cttggagcgc cgacgaaacg acaccttatt actgcatcga
      961 accttggatg gggccgccaa atgctcctga acatcggcgc ggcctgcacc tcgttccgcc
     1021 gctggaaacc ggcacgttca ccgtcagcgt ctcgatcaag taagcccgcg caaaaaccgc
     1081 gtttgccctt cccttttttc gtttcaacgt aagttctccg tcatgacgcc cgaactcttc
     1141 ttcttcgcga ttctgcccga tttgggcggt ggagagatga tgctcattct cctggtcatg
     1201 ctgctgcttt tcggtggcga caagatgccg caaatggcca agagcctcgc gaaaacgctg
     1261 cgcgaattta aacgcgccgc gagcgaagtg gagcg
//
LOCUS       DASPLT010001425         2826 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_221513, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001425 DASPLT010000000
VERSION     DASPLT010001425.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 2826)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2826)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2826
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_221513"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..717)
                     /locus_tag="VEA63_14525"
     CDS             complement(<1..717)
                     /locus_tag="VEA63_14525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HYD85277.1"
                     /translation="MKHLSSPEIWFVCGSQHLYGPGPLKQVAANAQQVAAGLAKAKSI
                     PLRIVYKALVTTPDEITQLCIDADADANCAGLILWMHTFSPAKMWIRGLSTLKKPFCH
                     LHTQFNRDLPWGEIDMDFMNLNQAAHGDREAGFLHTRLRLNRKVVVGHWSDPDVHARL
                     GAWVRAARAWHDWQGAKFARFGDNMRYVAVTEGDKVASELRFGYSVNGYGVGDLVAKL
                     AAISEADVSALCADYEKSYTV"
     gene            complement(861..2522)
                     /locus_tag="VEA63_14530"
     CDS             complement(861..2522)
                     /locus_tag="VEA63_14530"
                     /EC_number="2.7.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008741 - ribulokinase activity [Evidence
                     IEA];
                     GO_process: GO:0019569 - L-arabinose catabolic process to
                     xylulose 5-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulokinase"
                     /protein_id="HYD85278.1"
                     /translation="MFTLGLDYGTNSVRALIVRTSDGKEFGSAVVNYPSGAQGVLLDK
                     RDHHLARQHPGDYLFGLEKSIRAALAQAKKQRGFSADKIVGIGVDTTGSSPIPVDANN
                     APLALSKKFAKNLNAQCWLWKDHTAWREAAKITELAAQHRPQFIAKCGNTYSSEWFWS
                     KIWHCLAVDPKVFAAAHSWVELADWIPSVLAGVSDPTQIQRGVCMAGHKALYADDWGG
                     LPDKEFLALLDSKLADLRDRLYEKAHDATASAGQLSPAWAKKLGLRAGIPIAIGEMDV
                     HYGAIGSGVTEGTLVKIIGTSTCDCGVVSADKTVPDIAGICGIVKGAILPGYYGIEAG
                     QSAVGDIFKWWVEGVCQGNAALHAQLTKEAAQLAPAQSGLLALDWQNGNRTILVDQRL
                     SGLLLGMTLHTSRAEIYRALIEATAFGARAIIERIRDYGVPIDRVVCAGGIAEKNPLL
                     MQIYADVTGCTMLVAGSSQACALGSAISAAVLAGAHPDFPGAQKAMTSLKKISYKPKS
                     GAQKTYDELYRLYRQLHDAFGNVNKSADLTRVMKDLLDLKDAART"
BASE COUNT          517 a          785 c          991 g          533 t
ORIGIN      
        1 gacggtgtag cttttttcgt agtcggcaca gagcgcggaa acgtcggctt cggaaatcgc
       61 ggcgagtttt gcgacgaggt cgccgacgcc gtaaccgtta actgaatacc cgaagcgaag
      121 ctcgctggcg actttgtcgc cttcggtgac cgcgacgtag cgcatgttgt cgccgaaacg
      181 cgcgaacttc gcgccctgcc agtcgtgcca cgcgcgggcg gcgcgcaccc acgccccgag
      241 gcgcgcgtgg acgtcggggt cggaccagtg gccgacgacg accttgcggt tgagacgcag
      301 gcgcgtgtga agaaaacccg cctcgcgatc gccgtgcgcg gcctggttga ggttcatgaa
      361 gtccatgtcg atctcgcccc acgggagatc gcgattgaac tgggtgtgca ggtggcagaa
      421 cggcttcttc aacgtgctca gcccgcggat ccacatcttt gcgggcgaga acgtgtgcat
      481 ccatagaatg aggccggcgc agttcgcgtc ggcatcggcg tcgatgcaga gctgcgtgat
      541 ctcgtcgggc gtggtgacga gcgctttgta gacgatgcgc agaggaatcg atttcgcttt
      601 cgcgagaccg gcggcgactt gctgcgcgtt ggcggcgacc tgcttgagcg ggccgggccc
      661 gtagaggtgc tgcgaaccgc agacgaacca gatttcgggg gaggagaggt gtttcatgga
      721 gaagagtcga tcgaggtcgt ggaaaatctt tttctttctt ctttctcttt atctttctct
      781 ggatcgaaag gcggacgcag cgttgtgccg aaggccggga gaacgagaaa gattacgaga
      841 acgagaacga gtcggagggc tcaggtgcgc gcggcgtctt tgagatccaa cagatccttc
      901 atcacgcggg tgaggtcggc ggatttgttg acgttgccga acgcgtcgtg gagttgccga
      961 tacaagcggt agagttcgtc gtaggttttc tgggcaccgg acttcggctt gtaggaaatt
     1021 tttttcaatg aggtcattgc cttctgcgcg cccgggaaat ccgggtgcgc gccggcgagg
     1081 acggcggcgg agatcgcgga gccaagggcg cacgcttgcg aggagccggc gacgagcatg
     1141 gtgcaacccg tgacgtcggc gtagatctgc atgagcagcg gattcttttc cgcgatgccg
     1201 ccggcgcaga cgacgcgatc gatcggcacg ccgtagtcgc ggattcgctc gatgatggcg
     1261 cgcgccccga atgcggtggc ctcgatgagc gcgcggtaaa tttcggcgcg gctggtgtgg
     1321 agcgtcatgc cgaggagcag accggagaga cgctggtcga cgaggatcgt gcgattgccg
     1381 ttctgccaat cgagcgcgag caggccggac tgggccggcg cgagttgcgc ggcctctttc
     1441 gtgagttgcg catggagcgc ggcgttgccc tgacaaacgc cctcgaccca ccatttgaaa
     1501 atatcaccga ccgcggactg gccggcctcg atgccgtagt agccgggaag gatcgcgcct
     1561 ttcacgatgc cgcaaatgcc ggcgatgtcg ggcacggtct tgtcggcgga gacgacgccg
     1621 cagtcgcagg tcgaggtgcc gatgattttg accaacgtgc cctcggtcac gccgctgccg
     1681 atcgcaccgt agtgaacatc catttcaccg attgcgatcg ggatgccggc gcgcaggccg
     1741 agcttctttg cccacgcggg agaaagttgt ccggcggacg ccgtggcatc gtgcgctttt
     1801 tcgtagagcc gatcgcgcag atcggcgagt ttcgagtcga gcaacgcgag gaattccttg
     1861 tcgggcaagc cgccccagtc gtcggcgtag agcgctttgt ggccggccat gcaaacgccg
     1921 cgctggattt gcgtcgggtc gctgacgccg gcgaggacgg acggtatcca atccgccaat
     1981 tcaacccacg agtgcgcggc ggcgaacacc ttcgggtcga cggcgaggca gtgccagatc
     2041 ttggaccaga accactcgga cgaataagta ttgccgcact tggcgatgaa ctgcgggcga
     2101 tgttgcgcgg cgagctccgt gatcttcgcg gcttcgcgcc acgccgtgtg atctttccag
     2161 agccagcact gcgcgttgag gttcttcgcg aattttttgg agagcgcgag cggggcgttg
     2221 ttcgcgtcga cggggatcgg cgacgagccg gtggtgtcga cgccgatgcc gacgatctta
     2281 tcggcggaga aaccgcgctg ttttttcgcc tgcgcgagcg cggcgcggat ggatttctca
     2341 agaccgaaaa gatagtcgcc gggatgttgg cgcgcgaggt ggtgatcgcg tttatcgagc
     2401 agcacgcctt gcgcaccgct gggatagttg acgacggcgg agccgaattc cttgccgtcg
     2461 gaagtgcgaa cgatgagggc gcggaccgag ttggtgccgt aatcgagacc gagagtgaac
     2521 atggaaggac gggaggggaa cgaaggaacg gacgtgcaga gatgagaggt gcggaacggc
     2581 gggtcaacga agccgccgac gtgaccgtaa agccaagctc aggcggcgcg acggcgaggg
     2641 cggattgccg acgcgcggga attcaggagt cgttagcagc gtcgtcaaac gtcggtctca
     2701 ctcacgcgac tgaacgagga tgtaggccgg ggtcttcccg atcccaccgt ctttggtatc
     2761 gattcgcaaa ctacggcggt gtcaggagac cccaccctac aattcgtcac gcgttggccg
     2821 cgtagc
//
LOCUS       DASPLT010001426         1298 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_262790, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001426 DASPLT010000000
VERSION     DASPLT010001426.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1298)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1298)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1298
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_262790"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(160..762)
                     /gene="lexA"
                     /locus_tag="VEA63_14535"
     CDS             complement(160..762)
                     /gene="lexA"
                     /locus_tag="VEA63_14535"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HYD85279.1"
                     /translation="MLTEKQEAILDYIRGVQTERGIPPSTREIQRQFGYESQNAAMNH
                     LRALAKKEQIHQNDHGMWGLKVSAVQGHFELPIYGTIPAGVPSLQEQPPKETIAFDPA
                     IFRVRRPERLWGLEVHGDSMIDAHILDGDIAVLERREAKAGDIVAALVDETTTTLKRL
                     AYVKGKPVLKAENARYATIVPKDGLEIQGVLVGVIGRAKR"
     gene            complement(1010..>1298)
                     /locus_tag="VEA63_14540"
     CDS             complement(1010..>1298)
                     /locus_tag="VEA63_14540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD85280.1"
                     /translation="DQARAEPGKQGTRGGGRGDAQPSDAPTKAATPRPADQHEQRGPG
                     DPRDNFEQPYVHRNLRGGERDSDAAAEKAAKPVTPGPASHQGSRGGGRGDG"
BASE COUNT          221 a          492 c          335 g          250 t
ORIGIN      
        1 ccggtaggga cgggtctccg acccgtccgt tcggttgaaa ccgcgccaag atcacgccct
       61 ctctctgcgc acgaccgcag tgatgctcac gctttctctc actcgaacgc acccttccga
      121 gcgaacaccg ccacgttgcg caaaactcgc gcgcgccctt caccgcttcg ccctcccgat
      181 cacgcccacc aacacgcctt gaatctccaa accgtccttc ggcacgatcg tcgcatagcg
      241 cgcgttctcc gccttcaaca ccggcttgcc tttcacatac gccagccgct tcagcgtcgt
      301 cgtcgtctcg tccaccagcg ccgccacgat gtcgcccgcc ttcgcctcgc gtcgctccaa
      361 caccgcgatg tccccatcga ggatatgtgc atcgatcatc gaatcgccgt gcacctcgag
      421 tccccacaac cgctccggcc ggcgaactct aaaaatcgcc ggatcgaacg cgatcgtctc
      481 cttcggcggc tgctcctgca acgatggcac gcccgccgga atcgtgccgt agatcggcag
      541 ctcgaaatgc ccctgcaccg cgctcacctt caagccccac atcccgtgat cgttctggtg
      601 aatctgctct ttcttcgcca gcgctcgcag atgattcatc gccgcattct ggctctcgta
      661 accgaattgt cgctggatct cgcgcgtgct cggcggaatc ccgcgctccg tctgcacgcc
      721 acgaatgtag tcgagaatcg cttcctgttt ttcggtgagc atggtgcgtg ttcaggtgaa
      781 cacatgttcg aatgaacatc cgacgacgca cttggcaagc cctgcgtcca tcgtgggtcg
      841 caaagccgtg ccgccgcgca ggcttggagc gcagaaaacg cttccgcaaa acccgttcgg
      901 aagcgtcacc gaaaacaaaa accagcgaat caccagtaag gtggaacccg catccccgcg
      961 cgcggagaca gacccgcgac gagcaactcg ggccctgaat tcgtatcatt caaccatcgc
     1021 cgcgaccgcc tccgcgactg ccttggtgcg acgccggccc cggcgtcacc ggtttcgccg
     1081 ctttctccgc tgcagcgtcg ctgtcacgtt cgccgccgcg caaattccga tgcacatacg
     1141 gctgctcgaa attatcccgc ggatcgcccg ggccgcgctg ctcatgctga tccgccggac
     1201 gcggcgtcgc cgccttcgtc ggcgcgtccg acggctgcgc atcgccgcga cctccgccgc
     1261 gcgtgccttg cttgcctggt tctgcccgcg cttgatcg
//
LOCUS       DASPLT010001427         2134 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_241380, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001427 DASPLT010000000
VERSION     DASPLT010001427.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 2134)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2134)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2134
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_241380"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1008)
                     /gene="cheB"
                     /locus_tag="VEA63_14545"
     CDS             complement(<1..1008)
                     /gene="cheB"
                     /locus_tag="VEA63_14545"
                     /EC_number="3.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis-specific protein-glutamate
                     methyltransferase CheB"
                     /protein_id="HYD85281.1"
                     /translation="MEAPIRILIVDDSAFVRKVVREILASSPGIEVVGTARDGEEALE
                     MTASLEPHLVICDLRMPNMDGVRFVRRQMALRPLPILILSAAAQDAAEVVEALSAGAI
                     DVVQKPTALATDDLRMVRQALLEKVREAMSVPVGALTREFTPAAAVVKRGGETKVDVV
                     VVGISTGGPQALRRMLPLLPADFAVPIAVVLHMPVGYTALYAEKLNEICELTVKEAAD
                     GDVLRPGMVLIAAAGRHLIFHREASGEVIAQLTMQPLEKLHRPSADVLFRSAAEIYHD
                     HVLAVVMTGMGDDGTEGAAWVKAQGGTVITEAEKSCVIYGMPRSVAEAGLSDLVVPLE
                     EM"
     gene            complement(1017..1865)
                     /locus_tag="VEA63_14550"
     CDS             complement(1017..1865)
                     /locus_tag="VEA63_14550"
                     /inference="COORDINATES: protein motif:HMM:NF013867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CheR family methyltransferase"
                     /protein_id="HYD85282.1"
                     /translation="MRASGDPSPLLVPEPVPKLLRDLIHERTGLFFEPERFDTMLDKL
                     QPRLAENSVTSYLDYYYILKYDEKGPGEWRRVMDAFSVQETYFWREFDQIRALVETVV
                     PEWFKRHDRPLRIWSAACATGEEPYSIAMALEEAGWGKHPIEIHASDASEAALGKARA
                     AVYRERSFRALPSALRAKYFKTEREGEVLSRDIMERVKFRWANLMRPEDYADLSDLQA
                     VFCRNVFIYFSNAAITRVVSAFAQRMQPGSRLFIGASESLLKLTDAFELHDIRGAFVY
                     LRKPVA"
     gene            complement(1871..>2134)
                     /locus_tag="VEA63_14555"
     CDS             complement(1871..>2134)
                     /locus_tag="VEA63_14555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HYD85283.1"
                     /translation="ASQNIARQIKLITAANIGHSTAAQGMLTNLEEIRAVTLQNVAEG
                     SGAASGGAKPAQPEASKRGRGTLSGKKDAQRANVARANGSKRA"
BASE COUNT          392 a          822 c          530 g          390 t
ORIGIN      
        1 catttcttcc aacggaacca ctaaatcgct caggccggct tcagcaacgg agcgcggcat
       61 cccatagatg acgcaacttt tttccgcttc ggtgatgacc gtccccccct gcgccttcac
      121 ccacgccgcg ccttccgtcc cgtcatcccc catgcctgtc attaccaccg ccagcacatg
      181 gtcgtgataa atctccgccg ctgatcgaaa cagcacatcc gccgatggcc ggtgcagctt
      241 ctccagcggt tgcatcgtca attgcgcgat cacttctccg ctcgcctcgc gatgaaagat
      301 caaatgcctc cccgctgccg cgatcagcac catccccgga cgcaacacgt cgccgtccgc
      361 cgcctccttc accgtgagtt cgcaaatctc gttcaacttc tccgcataca acgccgtgta
      421 gcccaccggc atgtgcagca cgaccgcgat cggcaccgcg aaatccgccg gcaacaacgg
      481 cagcatccgc cgcagcgcct gcggcccgcc cgtggaaatt cccacgacca ccacatccac
      541 tttcgtctcg cctccgcgtt tcaccaccgc agccgccggc gtgaactccc gcgtcagcgc
      601 tcccaccggc acgctcatcg cttcgcgcac tttctccagc agcgcctgcc gcaccattcg
      661 caaatcgtcg gtcgccagcg ccgtcggctt ctgcacgaca tcgatcgcgc ccgcgctcaa
      721 cgcctccacc acttccgccg catcctgggc cgccgccgac aggataagga tcggcaaagg
      781 tcgcaacgcc atctgccgcc gcacaaagcg cacaccatcc atgttcggca ttcgcaagtc
      841 gcagatcacc agatgcggtt ccaagctcgc cgtcatctcc aaggcctcct caccgtcgcg
      901 cgccgtcccg accacctcga tgcccgggct gctcgcgagg atctcgcgca ccactttccg
      961 cacaaacgcg gaatcgtcga caatcagaat acggatcggg gcttccatgt gatccgtcag
     1021 gccaccggct tccgcagata aacgaaagca ccgcggatgt cgtgcaattc gaacgcatcc
     1081 gtcagcttca gcagcgactc cgaagcgccg atgaacaacc ggctgcccgg ctgcatgcgt
     1141 tgtgcaaacg cggatacgac ccgcgtgatc gctgcattcg agaaatagat aaacacgttc
     1201 cggcaaaaca ccgcctgcaa gtcgctcaaa tccgcgtagt cctccggtcg catcagattc
     1261 gcccagcgaa acttcacccg ctccatgatg tcgcggctca gcacctcgcc ctcccgctcc
     1321 gttttgaaat acttcgcgcg caacgccgac ggcagcgccc gaaacgaccg ctcgcgatac
     1381 accgccgccc tcgccttccc cagcgccgcc tcgctcgcgt cgctcgcgtg aatctcgatc
     1441 ggatgcttcc cccagcccgc ctcctcgagc gccatcgcga tgctatacgg ctcctcgccc
     1501 gtcgcgcacg ccgcgctcca gattcgcaaa ggccgatcgt gccgcttgaa ccactccggc
     1561 accaccgtct ccaccagcgc gcggatctgg tcgaactccc gccagaaata cgtctcctgc
     1621 accgagaagg catccatcac gcgccgccat tcgcccggac ctttctcgtc atatttgagg
     1681 atgtagtagt aatccagata cgacgttacg ctattctccg ccaggcgcgg ctggagcttg
     1741 tccagcatcg tgtcgaaacg ctccggctcg aagaacaagc cggtgcgttc atgaatcaag
     1801 tcgcgcagca gcttcggcac cggctccggg accaaaaggg ggctggggtc tccggacgcc
     1861 cgcatgaatg tcatgcgcgc tttgaaccgt tagcgcgcgc tacgttcgcc cgctgcgcat
     1921 cttttttccc cgaaagcgtg ccgcggccgc gcttgctcgc ttcaggctgc gccggcttcg
     1981 cgccgcctga ggccgcgccg ctcccctccg ccacattctg cagggtcacc gcgcgaatct
     2041 cctcgagatt cgtcaacatc ccttgggccg ccgtcgaatg cccgatgttt gcagccgtga
     2101 tcaacttgat ctgccgcgcg atgttctggc tcgc
//
LOCUS       DASPLT010001428         1103 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_77738, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001428 DASPLT010000000
VERSION     DASPLT010001428.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1103)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1103)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1103
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_77738"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(482..883)
                     /locus_tag="VEA63_14560"
     CDS             complement(482..883)
                     /locus_tag="VEA63_14560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD85284.1"
                     /translation="MRSTIEERLKAGDTFEAAAAAAAKAQSVKIDAKMLPAFTRRQPP
                     QDIDYSVFGALQRLEKGRVSDMIVTRDNGLIVYAADKKVPNLDNASPEFATTRSQIAS
                     ATAGIGASSYLNSWVEDELKKSDPVGATAAP"
BASE COUNT          200 a          267 c          449 g          187 t
ORIGIN      
        1 gagcgtgggt gcggtttcga tgggcggcgg agcggcgggc tcttgggcgc gggcgaagga
       61 agcgcaaagg cagaaggcga gggcagcgcg agcggtgaga cgcaacatgg gagagaggaa
      121 caagccggag attggaacgg agaacacggt ggctgtcgaa aagttactcg ggatcgaagc
      181 cggacggcgc ggcgcggatt atcgggcagg gccggagagc ttcgcgcgag agacggaggt
      241 tgccgcggaa agtttcgttg cgtcgaagga ttgcagccgg cgcgggcgaa gagtggggag
      301 gtgcaacgac atggcgcgcc gagtgtcggt ctcgcgcgag tgcgattcga gcgcaaacag
      361 gccgaacggt ttgagcgcgg gatcggcggt cggcgggcga aacaaaaagg gcgggtcgtg
      421 agacccgccc aagtggtgaa ggaagcgcgc gggatcgcgc gctaacgatg caagacgagg
      481 gtcagggcgc ggcggtggcg ccgacgggat cgctcttttt caattcgtcc tcgacccagc
      541 tattcagata ggagctggcg ccgatgcccg cggtggcgga ggcgatctgg ctgcgggtgg
      601 tggcgaattc cggcgacgcg ttgtcgaggt tcggcacttt tttgtccgcc gcgtaaacga
      661 taaggccgtt gtcgcgggtc acgatcatgt cggagacgcg gcctttttcg aggcgttgga
      721 gcgcgccgaa aacggagtag tcgatgtctt gaggcggctg gcggcgggtg aaggcgggca
      781 gcatcttggc gtcgattttc acagactgcg cttttgcggc ggcggcagcg gcggcttcga
      841 aggtgtcgcc ggctttcagg cgttcctcga tggtgctgcg cacggagcgg ccgagatcga
      901 cgaagcgttt gcgtttctca ttttcggtgt aatcggcggc gaccttctcg cgaacttcgg
      961 agagcggcgg cgtgcgcgtg ggctgtaggt cctcgaagaa gagaacgaca gcgccggctg
     1021 gagagttgac cgcgtcggag acggtgtggt cggcgttgag cttgaaggcc tccgcggaaa
     1081 ggagcgaggg atttcagcgc gag
//
LOCUS       DASPLT010001429         1175 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_118754, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001429 DASPLT010000000
VERSION     DASPLT010001429.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1175)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1175)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1175
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_118754"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..322
                     /locus_tag="VEA63_14565"
     CDS             <1..322
                     /locus_tag="VEA63_14565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD85285.1"
                     /translation="GIDAMAQLGADPAVCGVAFVNVNWAWSGGYTYLHRDIPMFLART
                     PEEFGEVAPGVNALLVDESIAPGLNVFAVEWCRQGMCLARRPGPCVPVPVPTINAQLA
                     AGRP"
     gene            complement(366..>1175)
                     /locus_tag="VEA63_14570"
     CDS             complement(366..>1175)
                     /locus_tag="VEA63_14570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD85286.1"
                     /translation="CEFAVGILLVAAPILHFTTQRRDPVLLRAPIGIVVYCFVAAMAS
                     PQPPEWTYFADVRYLTPIIPACIYVGVRAIEALPALPVAGRIALALILFGTNVSNVAL
                     AAVMPGAKAGPPRSTLLAYIAELRSPLPSPYAEAAKWINSYVAPGQSVFAYPEHTMYP
                     LMFHAGHAVYAWQFKADMRSQYPELPDIHFKNHAFPDYVFTFGPEGGAIRPVLGEFAK
                     RGVTYVPVAGLPVNGHDFTRPEVFWRSFKAAPIASVFEDGVTIFRRTDSAP"
BASE COUNT          220 a          362 c          411 g          182 t
ORIGIN      
        1 cgggatcgat gcgatggcac agctgggagc cgatccggcc gtctgtggtg tcgcgttcgt
       61 gaacgtcaac tgggcgtgga gcggcggata cacgtacctc catcgcgaca ttccgatgtt
      121 tctcgcgagg acaccggagg agttcggcga ggtggcccca ggcgtcaacg cgctcctcgt
      181 cgacgagagc atcgctcctg ggctgaacgt cttcgccgtc gagtggtgcc gtcagggcat
      241 gtgtctcgcg cggaggcccg gtccgtgcgt gcccgtgccc gtgccgacga tcaacgcgca
      301 actcgctgcc ggacgcccgt aagcgcggcg agagaacgtc tctgtaaagg ggtctttccc
      361 acaggtcagg gcgccgagtc ggtccgacgg aagatcgtga ctccatcctc gaagacgctc
      421 gcgatcggtg ccgccttgaa cgaccgccag aacacctcgg gccgcgtgaa gtcgtggccg
      481 ttcaccggaa gccccgcgac gggcacgtac gtcacgcccc gcttcgcgaa ctcaccgagg
      541 acgggacgaa tggcgccgcc ctcgggaccg aacgtgaaga cgtagtccgg gaacgcatgg
      601 ttcttgaagt ggatgtccgg cagctcaggg tactgcgaac gcatgtctgc cttgaactgc
      661 cacgcgtaga cggcgtgccc cgcgtggaac atgagggggt acatcgtgtg ctcggggtac
      721 gcgaacaccg actgccctgg cgcaacgtac gagttgatcc acttcgctgc ctccgcatag
      781 gggctgggca gcggcgagcg aagctccgcg atgtaagcga gcagcgtgga acgcggcggt
      841 ccggccttcg cgccgggcat caccgccgcg agggcgacat tgctgacgtt cgtgccaaag
      901 agaatgaggg cgagggcgat acgtccggcg acgggcaagg ccggtagcgc ctcgatcgcc
      961 cttacgccga cgtagatgca cgcggggatg atgggggtga ggtatcgcac gtccgcgaag
     1021 taggtccact caggaggttg cggcgaagcc atcgcggcaa cgaagcagta gacgacgatc
     1081 ccgatgggcg ctcgcaacag cacggggtcg cgccgctgcg tcgtgaagtg aagaatgggc
     1141 gccgcaacga gcaagatacc aaccgcgaac tcaca
//
LOCUS       DASPLT010001430         4642 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_221587, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001430 DASPLT010000000
VERSION     DASPLT010001430.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 4642)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4642)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4642
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_221587"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            364..1359
                     /locus_tag="VEA63_14575"
     CDS             364..1359
                     /locus_tag="VEA63_14575"
                     /inference="COORDINATES: protein motif:HMM:NF012607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HYD85287.1"
                     /translation="MSTAALLMSASDLPVVYDENVLARLRSSLGRRLTVIAPAIHWRQ
                     HTLELADVDVVFSGWGAPRMDEAFLQHMPALRAIFYAGGSVGYFVTDSVWQRGIRLTS
                     AQAVNAIPVAEYATSALLLGLKRVWSHAAAVRAQRTFPRERPLPGAFRSKVGLVSFGM
                     IARLVRDRLLASDVDVLVYDPFLSPSEAAAARVRLVGLDELFATADAVSLHAPHRAET
                     TALIRGRHFALMKEGAFFLNTARGEIVAESEMIAVLQRRPDLFAMLDVTAPEPPLTDA
                     LLYSLPNVVVTPHLAGSVGPECARMGHAMVDEFERYCAGQPLKWEIDAQRAALLA"
     gene            1396..3204
                     /locus_tag="VEA63_14580"
     CDS             1396..3204
                     /locus_tag="VEA63_14580"
                     /inference="COORDINATES: protein motif:HMM:NF012686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD85288.1"
                     /translation="MITFYDYGIIAGYLLFMLGIGYAFRRMSQNTSDYFRAGGAMPWW
                     LTGASAWIFSFSAWTFTGAAGKVYESGTLVLVVFYATIVALAFAMAFTCVRFRRMRVI
                     TWMEAVRQRYGSGTEQFYTWVMLLLRLFFAGVSLNAIGVFLSAVFHVEMNTTLIVLGI
                     VVTIVAFAGGSWAVLASDFVQAFLIVTITLCTAILVLMHPEIDGVGGLLEKVPSAHLD
                     WSELARPQLIFLWILGTLWLKFSDENNMERATMYLMTRSDRDARLMVSIPLIGMLVGP
                     LIWFIPSMAATILHPDLASEYPGLKQPHEAAFLAVSLDVLPQGLLGLLLSAMIGATVT
                     AMDAGLNKNVGIGIRSLYKPVFRPHASERHLLVAGKICTLLFGAGIIVFALLVNRFRG
                     ANLFDLVNQLAASLLIPFALPLILGLFYKRTPGWSAWSTAVIATTTSQVLVTLISPQL
                     VQAWMGWSDPLTPDETTYLALAVSSLGGTVLIGTAWFFFTSLFYHRSPLEHRQQVDGF
                     FANLATPLNKDGVAGVQASVYRLLGALCLVYGGFILALMLIPNPLIGRLCFLFCGGTI
                     FLAGAILFKAGKERRTLNSPDADLAPAVESASARQQ"
     gene            3201..>4642
                     /locus_tag="VEA63_14585"
     CDS             3201..>4642
                     /locus_tag="VEA63_14585"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: protein motif:HMM:NF014156.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HYD85289.1"
                     /translation="MTTPLLAQHTFGDTVVRYPRDPATGRVGLELLPATELARAMPRR
                     TTLRGQPFIDAIPGNDAWPAAAIDSLVQFKLVGDAYPGGFAQGHTLRQSPGIDTFNLA
                     TQRVESHASDTTIVTTLVRADGCRIEHRLTARDGEPAFEITSTFVNASAAPVALELLS
                     SFSFGGITPFASDDAPDRLRVHRFRSGWSAEGRLETRSIEELHLERCWSGAAAFSERF
                     GQIGSMPVRRWFPFVALEDTAAGVVWGAHLAWAGSWQLEVYRKNDDVCLSGGLADREF
                     GHWLKTLAPGETLTTPTAYVSCVRGSLDDLCDRLTSAQHRAADTHPAVERDLPIVFNE
                     WCTTWGSPSEASVASLADRLAGTDVRYFVIDAGWYQGDAGDWGNAHGDWIPSAKLFPR
                     GIEAAAAAIRDRGLIPGLWFEMETVGDASTAFSLVDLLLQRDGLPITVRQRRFWDLRK
                     PRAVEYLAERVIGLLRRAGFGYLKIDYNET"
BASE COUNT          723 a         1581 c         1374 g          964 t
ORIGIN      
        1 ggattccgag gggttcggat aacgaatggt ctcatttacg ggtgagctga gggttggtga
       61 ggtaggcaac cgagcggaag caatcgcttc caatcggaag gcaacgtggg gtctgcggct
      121 gcgggacatt cgggaaaacc gtttcgaact ccatctccgc gctgcttagc agttaagcga
      181 cggagccctt cgacagccgt tttcggcagg gaccgttcgt agttgccgcg cggttacgcc
      241 gtcagagcgc ggcggcgtgc ctgcgctgct taaccggtaa gcagggtcga actggaatgg
      301 gccgccgccg cgttcaacct tcggacgttc gccgccgtcc gcggccaacg ctctccccct
      361 ttcatgtcga ccgctgcctt gttgatgtcc gcttccgacc ttccggtcgt ctacgacgaa
      421 aacgtgctcg cgcgcctccg ctcctcgctc ggccgccgcc tcaccgtgat cgcccctgcc
      481 attcattggc gccagcacac gctcgagctc gccgatgtcg atgtggtctt ctccggctgg
      541 ggagcgccgc ggatggacga agcgtttctt cagcacatgc cggcgttacg cgccatcttc
      601 tacgcaggcg gctcggtcgg ttactttgtg acggattcgg tgtggcagcg cggaattcgt
      661 ctcacctcgg cgcaggccgt gaatgcgatt cctgtcgcgg aatacgcgac ctccgcgttg
      721 ctgctcgggc tgaaacgcgt gtggtcacac gcggccgccg tccgcgcgca gcgaacgttt
      781 ccgcgcgaac gcccgcttcc cggcgccttt cgctcgaagg ttggcctcgt ttccttcggc
      841 atgatcgcgc gcctcgtccg cgatcgcctg ctcgcgtcgg acgtcgacgt gctcgtctac
      901 gacccgtttc tttcgccgag tgaagcagcc gcggcgcgcg tccggctggt cgggctcgac
      961 gagctttttg cgacggcaga cgccgtttca ttgcatgcgc cgcatcgcgc ggaaacaacg
     1021 gcgttgatcc gcggccggca cttcgcgctg atgaaggaag gtgctttttt tctcaacacg
     1081 gcgcgcggcg agatcgtcgc ggaatcggaa atgatcgcgg tcctgcaacg tcgccccgac
     1141 ctgttcgcga tgttggacgt cacggcgccg gaacctccgc tcaccgacgc cctgctctat
     1201 tcgcttccga atgtcgtggt gacaccgcac ctcgcgggct ccgtcggtcc ggagtgcgcg
     1261 cgcatgggcc acgcgatggt cgacgagttt gaacgctatt gcgccggaca accgctcaag
     1321 tgggaaatcg atgcgcagcg cgccgccctg ctcgcctgat ttcatcgctt cgttttcggc
     1381 cgacaaaatc acctcatgat cactttctac gactacggaa tcatcgccgg ctacctgctg
     1441 ttcatgctcg gcatcggcta cgcgtttcgc cggatgagcc agaacacgtc cgactatttc
     1501 cgcgccggcg gcgcgatgcc gtggtggttg accggagcgt cggcgtggat cttcagcttc
     1561 agcgcgtgga cgttcaccgg cgcggccgga aaagtttacg agtccggcac gctcgtgctc
     1621 gtcgtgttct acgccacgat cgtcgcgctc gccttcgcga tggcgttcac gtgcgtgcgc
     1681 tttcgccgca tgcgggtgat cacctggatg gaagccgtgc gtcagcgcta cggcagcggc
     1741 acggaacagt tctacacgtg ggtcatgctc ctgctgcggc tgtttttcgc cggcgtcagt
     1801 cttaacgcga tcggcgtgtt cctctccgcg gtctttcacg tcgagatgaa caccacgctc
     1861 atcgtgctcg gaatcgtggt gacgatcgtc gcgttcgccg gcggttcgtg ggccgtgctc
     1921 gcgagcgatt tcgtgcaggc gtttctcatc gtcaccatca cgctctgcac cgcgattttg
     1981 gtattaatgc atcccgagat cgacggcgtc ggcgggctgt tggaaaaagt cccctccgcc
     2041 catctcgact ggtccgaact cgcgcgaccg cagctcatct ttctttggat cctcggcacg
     2101 ctctggctga aattttccga cgagaacaac atggagcgcg ccaccatgta tctcatgacg
     2161 cgcagcgatc gcgacgcgcg cctcatggtt tcgattccgc tcatcggcat gctggtcgga
     2221 ccgctcatct ggtttatccc ctcgatggct gcgaccattc tgcatccgga tctcgcgagc
     2281 gaatatcccg gtttgaaaca accgcacgaa gccgcgtttc tcgccgtctc gctcgatgtg
     2341 cttccgcaag gcctgctcgg tctgcttctt tcggcgatga tcggcgcgac cgtcactgcc
     2401 atggacgccg gtctcaacaa gaacgtcggc atcggcatcc gcagcttgta taaaccggtg
     2461 ttccgcccgc acgcctccga acgccatctg ctcgtcgccg gaaaaatctg cacgctcctt
     2521 ttcggcgccg ggatcatcgt gttcgcgctg ctcgtaaatc gttttcgagg cgcgaatctc
     2581 ttcgatctcg tgaaccaact cgccgcgagc ctgctcatcc cgttcgcgct gccactcatt
     2641 ctcggcctct tctacaaacg cacgcccggc tggtccgcgt ggagcaccgc ggtcatcgcc
     2701 acgaccacgt cgcaggtgct cgtcacgctg atttctccgc agctcgttca agcgtggatg
     2761 ggttggagcg atccgctcac ccccgacgaa accacttacc tcgcgctcgc ggtgagctcg
     2821 ctcggcggca ccgtgctgat cggcaccgcg tggttctttt tcacgtcgct cttctatcac
     2881 cgctcaccac tcgaacaccg gcagcaggtc gatggcttct tcgcgaatct cgcgacgccg
     2941 ttgaacaagg acggcgtcgc tggcgtgcag gcctccgtct atcgactgct cggcgccctc
     3001 tgtctcgtct acggcggctt cattctcgcg ctcatgctca ttccgaatcc gctgattggc
     3061 cggctgtgtt ttcttttctg tggcggcacg atcttcctcg ccggagcgat tcttttcaaa
     3121 gccggcaaag aacgccgcac attgaattca cccgacgcag acctcgcgcc cgcggtcgag
     3181 tcggccagcg cgcgtcagca atgaccacac ccttgctcgc gcaacatacc ttcggcgaca
     3241 ccgtcgtccg ctatccacga gatcccgcca ccgggcgcgt gggactggaa ttgctgcccg
     3301 caaccgagct cgcccgcgcg atgcctcggc gcacgacctt acgcggccag ccattcatcg
     3361 acgcgattcc cggcaatgat gcgtggcctg ccgccgcgat cgattcgctg gttcaattca
     3421 aactcgtcgg cgacgcctat cccggcggct tcgcgcaagg ccacacgctg cgtcaatcgc
     3481 cgggcatcga cacgttcaat ctcgcgacgc agcgcgtcga gtcgcacgcc agcgacacga
     3541 cgatcgtcac caccctcgtg cgcgccgacg gttgccgcat cgaacatcgg ctcaccgctc
     3601 gcgacggcga accggccttc gaaatcacat ccaccttcgt caacgcgtcc gccgctccgg
     3661 tggcgctcga gctcctgagc agtttctcgt tcggtggcat cacgcccttc gcgagcgacg
     3721 acgcgccgga ccggctgcgc gtgcaccgct ttcgctccgg ttggagcgcc gaagggcggc
     3781 tcgaaacgcg cagcatcgaa gagcttcatc tcgagcgttg ctggagcggg gccgccgcgt
     3841 tcagtgaacg ctttggccag atcggatcga tgccggtgcg gcgttggttt ccgtttgtcg
     3901 cgctcgagga caccgccgcc ggcgtcgtct ggggcgccca ccttgcgtgg gctggctcgt
     3961 ggcagctcga ggtgtatcga aaaaacgacg acgtgtgcct ttccggcggg ctcgcagacc
     4021 gcgagttcgg ccactggttg aaaacactcg cacccggcga gacgctgacc acgcccaccg
     4081 cttacgtgtc atgcgtgcgc ggttcgctcg acgacctctg cgatcggctc acctcggcgc
     4141 aacaccgcgc cgccgacact catcccgccg tcgaacgcga cctccccatc gtcttcaacg
     4201 aatggtgcac cacgtggggc agcccgagcg aagcgtctgt cgcctcgctc gcggaccgtc
     4261 tcgccggcac cgacgtgcgt tacttcgtga tcgatgccgg ctggtatcaa ggcgacgccg
     4321 gcgactgggg caacgcgcac ggcgactgga ttccgagcgc gaaacttttc ccacgtggca
     4381 tcgaagccgc cgcggctgcc attcgcgacc gcggcctcat ccccgggttg tggttcgaaa
     4441 tggaaaccgt cggtgacgca tcgaccgcat tctcgcttgt cgatcttctg cttcaacgcg
     4501 acggactgcc gatcacggtc cgccagcgtc gcttctggga tctgcgcaag ccgcgcgccg
     4561 tcgaatatct cgccgaacgc gtgatcggac tcctccgtcg cgccggcttt gggtatttaa
     4621 aaatcgacta caacgaaacg at
//
LOCUS       DASPLT010001431         1003 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_77765, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001431 DASPLT010000000
VERSION     DASPLT010001431.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1003)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1003)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1003
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_77765"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            92..>1003
                     /locus_tag="VEA63_14590"
     CDS             92..>1003
                     /locus_tag="VEA63_14590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD85290.1"
                     /translation="MNISRRTFIAQTALATAGGLAAPYIRGSNRAAEPPIVGTGAHRF
                     EYVHDWLVPPEGMAWGDTHGVCQDSEGFIYVAHTVHGSSQRPEAIAVFDPEGKFVRAF
                     GAEFRGGAHGLDIRREQGVEVLYHCDTARNRVVKTSLRGEVLWEKAYPDDVPAYAAAR
                     IKYTPTNVAFAPDGDFFIGDGYGSHHLLKFSGDGDFLGEVGRAGGSGDGEINNPHGQW
                     VDLRGAEPVLVVADRGNRRLQTFTLDGRHLRTIKDETRLRMPCGFDVRGDLMVCPDLD
                     SQVCLLDRDYNVIAQLGDGQSENGKVGS"
BASE COUNT          193 a          285 c          339 g          186 t
ORIGIN      
        1 agttccaagg cctcgatcaa aagctcaccg gcgtggagcc ggcacgcgtc atcgaagatc
       61 tcatcgttta gaaactacgt tgtcgtcgca catgaacatt tcgcgtcgca ctttcatcgc
      121 tcagaccgcg ctcgccactg ccggcgggct cgccgcgccg tatattcgag gtagcaaccg
      181 cgcggcggag ccgccgatcg tcggaaccgg tgcacatcgt tttgaatacg tgcacgattg
      241 gctcgtgcca ccggaaggaa tggcgtgggg cgacacgcac ggtgtttgtc aggacagcga
      301 agggttcatc tacgtggcgc acacggtgca cggctcgagt cagcggccag aagccatcgc
      361 ggtgttcgat cccgagggga aattcgtgag ggcgtttggc gcggaatttc gcggcggcgc
      421 gcacggtttg gacataaggc gcgagcaggg tgtcgaggtg ttgtatcact gcgacaccgc
      481 tcgaaatcgc gtggtgaaaa ccagcctcag gggcgaggtg ttgtgggaaa aagcgtatcc
      541 cgacgacgtt cccgcctatg cggcagcgcg catcaaatac acgccgacga acgtcgcctt
      601 cgcgcccgac ggcgacttct tcattggcga cggttacggt tcgcaccact tgctgaaatt
      661 ttcgggggac ggcgatttcc tcggtgaggt cgggcgcgcg ggcggaagcg gtgacggcga
      721 gatcaacaac cctcacgggc agtgggtgga cctgcgtgga gctgagccgg tgctggttgt
      781 ggccgatcgc ggcaatcgtc ggctgcaaac cttcacgctc gacggccggc atctgcgcac
      841 gatcaaggac gagacgcggc tgcgaatgcc gtgcggcttc gatgtgcgag gcgacctgat
      901 ggtgtgcccg gatctcgaca gccaggtgtg tttgttggat cgcgactaca acgtgatcgc
      961 acaactcggc gatggccaaa gcgagaacgg caaagtcggc tcg
//
LOCUS       DASPLT010001432         2123 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_241392, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001432 DASPLT010000000
VERSION     DASPLT010001432.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 2123)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2123)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2123
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_241392"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            321..1187
                     /gene="ilvE"
                     /locus_tag="VEA63_14595"
     CDS             321..1187
                     /gene="ilvE"
                     /locus_tag="VEA63_14595"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009507826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain-amino-acid transaminase"
                     /protein_id="HYD85291.1"
                     /translation="MKIYLDGKFVDDADAKISVFDHGLLYGDGVFEGIRLYGGNVFRL
                     DEHLERLEYSAKAILLKIPLTRQELAEATCETCRQNNLKDAYIRLVITRGVGDLGLAP
                     WLCPKPTVFIIASKISLYPQEHYDNGLSIVTVPTRRINPAALPPTVKSLNYLNNILGK
                     IEAKQFGALEAIMLNDQGYVAECTADNVFIVHKGEIITPAASQGALKGVTRGTIFDVA
                     QELGIPIREANMTRYDVWCADECFLTGTGAEVIPVVKLDGREIGTGKPGAITQRVLAS
                     FRRRVLVEGTRI"
     gene            1242..1868
                     /locus_tag="VEA63_14600"
     CDS             1242..1868
                     /locus_tag="VEA63_14600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007363442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrB/UvrC motif-containing protein"
                     /protein_id="HYD85292.1"
                     /translation="MAQPVCRSGASEKEPPRVLPALASVGEGGMVGAKKFYESMANPL
                     KCDLCSKPATVHLTQIVNNKVHKVDLCEECAQAKGVTDPSGFSLADLLLKASLNPEPA
                     AIAGVRCEQCGFTPGDFKKHGRFGCPACYETFKGLVEPMLDSMHKGTAHTGKVPQKAL
                     ARRSLHERLSKLELDLTAAIKSERYEDAARCRDEINQVKQTFGQTPTR"
     gene            1873..>2123
                     /locus_tag="VEA63_14605"
     CDS             1873..>2123
                     /locus_tag="VEA63_14605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP--guanido phosphotransferase"
                     /protein_id="HYD85293.1"
                     /translation="MTIASLIDAPSELTDSSSSKTAVVLMTRIRLARNLASTPFPGWA
                     KPARRAEIFQACREALAVTSPMKRGVSVSVDELSDLEKQ"
BASE COUNT          439 a          589 c          701 g          394 t
ORIGIN      
        1 cgatgagggt gaacccggcg cgaacggcat gcgaacgaag ggggttgcgc atataaagga
       61 ggaaaagcgg gagccggcga gggtcacggc aactaatttg cgggacaagc tgaaactcgg
      121 atgatgacgc tggacgagca cgagtgcgga gcttcgagct gcgatcggaa taccgtttgt
      181 catacaatag atgacaaacg ggatcggggt cagggatggt tgacggatgc agcgcgcggt
      241 aaatttgcgg agttgctggc gtgggtgcgc gctgcgcggg gctggacaaa actttttttg
      301 cgccgctaat ctggcgggtc atgaaaattt atctggatgg caaattcgtc gacgatgccg
      361 acgccaagat ttccgtgttc gatcacggac tgctctacgg cgatggggtc ttcgagggca
      421 tccggcttta tggcggcaac gtgtttcggc tcgatgagca cctcgagcgg ctggaatact
      481 cggcgaaggc gatcttgttg aagatcccgt tgacgcggca ggagctggca gaggcgacgt
      541 gcgagacgtg ccggcagaat aacctgaagg atgcctacat ccggttggtg atcacgcgtg
      601 gcgtgggcga tcttggcctg gcgccgtggc tttgtccgaa gccgacggtg ttcatcatcg
      661 cgagcaagat ttcgctttat ccgcaagagc actacgacaa cggcttgagc atcgtcacgg
      721 tgccgacgcg ccggatcaat ccggcggcgc tgccgccgac ggtgaagtcg ttgaactacc
      781 tcaacaacat cctcggcaag atcgaggcga agcagtttgg cgcgctcgaa gcgatcatgt
      841 tgaacgacca gggttacgtc gcggagtgca cggcggacaa cgtgttcatc gtgcacaagg
      901 gcgagattat cacgcccgcg gcctcgcaag gcgcgttgaa gggcgtgacg cgcggcacga
      961 ttttcgacgt ggcgcaagag ttggggattc cgatccgcga ggcgaacatg acgcgctacg
     1021 acgtctggtg cgccgacgaa tgtttcctga cgggcacggg cgcggaggtg attccggtgg
     1081 tgaagctcga tggccgtgag atcggcacgg gcaagccggg cgcgatcacg cagcgcgtgc
     1141 tggcgtcgtt tcgtcgccgc gtgctggttg aaggcacgcg gatctaaggc tcgcgaaaac
     1201 gcgcgcgcgt gtcgtcgatc ggtgcgacac gcgtgcgcct aatggcgcag ccggtgtgca
     1261 ggagcggcgc gtcggaaaaa gaaccaccga gagttctccc agcacttgcc agcgtgggag
     1321 agggtggcat ggttggtgca aagaaattct acgaatccat ggccaatccg ctcaaatgcg
     1381 acctttgctc caaacctgcc acggtgcatc ttacgcagat cgtcaataac aaggttcaca
     1441 aagtcgatct gtgcgaagag tgtgcccagg ccaagggcgt gactgatccc agcggcttct
     1501 cgctcgcgga tctcctgctc aaggcgtcgc tcaatcccga accggcggcg atcgccggtg
     1561 tgcgttgcga gcagtgcggg tttacgccgg gcgatttcaa gaaacatggc cgcttcggct
     1621 gtccggcctg ctacgagacc ttcaaaggtc tcgtcgagcc gatgctcgac agcatgcaca
     1681 aaggcacggc ccacacgggc aaggttccgc aaaaggccct tgcccgtcga tccctccacg
     1741 aacgtctttc caaactcgag ctcgacctca ccgctgcgat caaatcggag cgttacgagg
     1801 acgccgcgcg ctgccgcgat gaaatcaacc aagtcaaaca aacctttggg caaaccccga
     1861 cgcgttaagc ccatgacgat cgcttcgctc atcgacgcac cctcggagct gacggactcc
     1921 tccagctcca aaacggcggt cgtgttgatg acgcggatac gtctcgcgcg gaatctggcg
     1981 agcacgccgt tccccggttg ggcgaagcct gcgcggcgcg cggagatttt tcaggcgtgc
     2041 cgcgaagcgc tcgcggtgac ttcgccgatg aagcgaggcg tgagcgtgtc ggtcgacgag
     2101 ctttccgatt tggagaaaca gat
//
LOCUS       DASPLT010001433         1237 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_118824, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001433 DASPLT010000000
VERSION     DASPLT010001433.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1237)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1237)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1237
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_118824"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..391)
                     /locus_tag="VEA63_14610"
     CDS             complement(<1..391)
                     /locus_tag="VEA63_14610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HYD85294.1"
                     /translation="MADFPKLDLTQRPRRLRRTASLRALVQETVLRPADFIAPLFVVE
                     GKAAPEAIASMPGVSRFNIADLVKECRALAKLGVPAVALFPKLEARLKDDEGSAALDE
                     DGLILRTVRAVKKAVPQIAVMTDIALDP"
     gene            complement(626..>1237)
                     /locus_tag="VEA63_14615"
     CDS             complement(626..>1237)
                     /locus_tag="VEA63_14615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007363413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-coenzyme A synthetase"
                     /protein_id="HYD85295.1"
                     /translation="FGVVPKVVDDNGKEVLRNSGGKLIITKPWPSMLRTLWGDDDRYQ
                     KAYWSEFKGVYFTGDGARQDEDGYFWIVGRIDDVLNVSGHRIGTAEVESALVSHPAVA
                     EAAAVGKPDELKGQALVVFVTLKAGHAASDELKEKLRGHVGKEIGSLAKPDVIRFAAA
                     LPKTRSGKIMRRILKEIAVGGSIKGDTTTLEDFSVVASLQAEE"
BASE COUNT          225 a          443 c          340 g          229 t
ORIGIN      
        1 agggatcgag cgcgatgtcg gtcatcaccg caatctgcgg cactgctttt ttcactgcgc
       61 gcaccgtgcg caaaatcaat ccgtcctcat ccagcgccgc cgagccctcg tcatccttca
      121 accgcgcttc caatttcgga aacagcgcca ccgcgggcac gccgagcttc gcgagcgcgc
      181 gacactcctt caccaaatcg gcgatgttga agcgtgatac accgggcatc gacgcgatcg
      241 cttcgggagc ggccttgcct tcgaccacga acagcggcgc gataaaatcc gccggccgca
      301 gcaccgtctc ctgcacgagc gcgcggagac tcgcggtgcg ccgcaaccgc cgcggacgtt
      361 gcgtaagatc gagtttgggg aaatcggcca tggtgaagaa gcgtgcaacc tagcacacgc
      421 gtccgccgcc gcgccagaac tatgagcgtc cgaaacgtag cggcaggcct ccgcgtctgc
      481 ctgcttccgt ccgctgcgcg cgcacaacgc agctgcgatg cccgagccca cacagccccg
      541 cccgtcccat ctccatcgcc atcgcgagca acagcaggcc cgaaggcctg ctgctgccgc
      601 agatcgcgcc ggcgcgtggc tgcgcttact cctccgcctg caaactcgcc acaacgctga
      661 aatcctcgag cgttgtcgtg tcgcctttga tcgatccgcc gacggcgatt tccttgagga
      721 tgcgccgcat gatttttccg ctgcgggttt tcggcagcgc cgccgcaaag cggatgacat
      781 ccggcttcgc gagcgagccg atttctttcc caacgtggcc gcgcaacttt tccttcagct
      841 catcgctcgc cgcatggccc gctttcaacg tgacgaacac cacgagtgcc tggcctttca
      901 actcgtccgg tttgccaacc gccgcggctt ccgcgaccgc cggatgcgag acgagcgcgc
      961 tctccacttc ggcggtgccg atgcgatggc cagagacgtt gagaacatcg tcgatgcggc
     1021 caacgatcca gaagtagcca tcctcgtcct gacgcgcacc gtccccggtg aaatacacgc
     1081 ctttgaattc gctccaatac gctttttggt agcgatcatc gtcgccccac agcgtgcgca
     1141 acatcgacgg ccacggcttc gtaatgatga gttttccccc ggaattacgc agcacttcct
     1201 tgccgttgtc gtcgacgacc ttcggcacca cgccgaa
//
LOCUS       DASPLT010001434         1285 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_263043, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001434 DASPLT010000000
VERSION     DASPLT010001434.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1285)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1285)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1285
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_263043"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..432
                     /locus_tag="VEA63_14620"
     CDS             <1..432
                     /locus_tag="VEA63_14620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012372918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HYD85296.1"
                     /translation="TTAWGEVSYGRQTVPEFNAGVDIRPLPGMEPKDRLGVIVASERV
                     SVRDNLPFSVPGGRLVVFGTGDLVANSRIADVANQNIFLGAVNWTIGRDTTFNIPARP
                     IERFQLSLSASELSRLRYTLLLALPAAAALLGLIVYWTRRS"
     gene            549..>1285
                     /locus_tag="VEA63_14625"
     CDS             549..>1285
                     /locus_tag="VEA63_14625"
                     /inference="COORDINATES: protein motif:HMM:NF025598.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4340 domain-containing protein"
                     /protein_id="HYD85297.1"
                     /translation="MRTKVTLVLLLLNVALFFFIFQFERRWRTEDTWKEARRRVLGPE
                     AADIRSLEIKGPAQSLSLVRRGDTWFITTPLEWPARDTAVSLILNELQLLEHVTSFYV
                     RDLAKNGQTLADYGLDEPKLTVSFTSGNSGDGGRTTLLRIGDTTKDGMRLYLLSSNGE
                     RIHVVNQSLARNLALTFEELHDDRVFTIPVFEAQSLNLQTAAPSSLRIRLRRDASRWT
                     FETPIIARASKNATELAINALNTLRIKS"
BASE COUNT          245 a          516 c          303 g          221 t
ORIGIN      
        1 acgaccgcgt ggggtgaagt cagctacggc cgccaaaccg ttcccgagtt caacgccggc
       61 gtcgacatcc gtcccctgcc aggcatggag ccgaaagatc gtctcggcgt catcgtcgct
      121 tccgaacgcg tctccgtgcg cgacaatctt cccttcagcg ttcccggcgg tcgcctcgtc
      181 gtcttcggca cgggcgatct cgtggccaat agccgcatcg ccgacgtcgc caaccaaaac
      241 atttttctcg gcgccgtgaa ctggacgatc ggccgcgaca ccaccttcaa catccccgcc
      301 cgtcccatcg aacgcttcca gctctcgctc agtgccagcg agctctcgcg cctgcgctac
      361 accctcctcc tcgccctccc cgccgccgcc gcgctgctcg gtctcatcgt ctattggacg
      421 agacggagct gacgctccgc gcaaccaaga gcccagagct gagagtccag agcccaaacc
      481 agaaacaccc gaaccctcgc ctccgagctc tcagctctag accctcagct ctcagctctc
      541 tccgcctcat gcgcaccaaa gtcacgctcg tcctcttgct cctgaacgtc gcgctcttct
      601 tcttcatctt ccaattcgag cgacgctggc gcaccgaaga cacttggaag gaagcccgcc
      661 gccgcgtgct cggccccgaa gccgccgaca tccgttccct cgaaatcaaa ggccccgcgc
      721 aaagcctctc gctcgtccgg cgcggcgaca cgtggttcat caccacgccg ctcgaatggc
      781 ccgcgcgcga caccgccgtc agcctcattc tcaacgagct ccagctcctc gaacacgtca
      841 ccagtttcta cgttcgcgat ctcgcgaaaa acggccagac gctcgccgac tacggcctcg
      901 acgaacccaa actcactgtc tccttcacct ccggaaacag cggcgacggt ggccgcacga
      961 cgctcctccg catcggcgac acgacgaagg acggcatgcg cctgtatctg ctttcctcca
     1021 acggcgaacg cattcacgtc gtcaaccaaa gcctcgcccg caacctcgcg ctcacgttcg
     1081 aggaactcca cgacgaccgt gttttcacga tcccggtttt cgaagcgcaa tcgctcaacc
     1141 tccaaaccgc cgcaccctcg agtctccgca ttcgcctccg ccgcgacgcc agccgctgga
     1201 ctttcgagac accgatcatc gcccgcgcca gcaagaacgc caccgagctc gccatcaacg
     1261 cgctcaacac ccttcgcata aaatc
//
LOCUS       DASPLT010001435         2226 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_56582, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001435 DASPLT010000000
VERSION     DASPLT010001435.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 2226)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2226)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2226
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_56582"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..277)
                     /locus_tag="VEA63_14630"
     CDS             complement(<1..277)
                     /locus_tag="VEA63_14630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HYD85298.1"
                     /translation="MLPSEAPAPAASIRPVKKLMAANRSEIAVRIFRAGTELGLRTVA
                     IFAHEDRLSIHRYKADEAYQVGQGKGPVAAYLDIESIVAVAKEKGVDA"
     gene            complement(465..806)
                     /locus_tag="VEA63_14635"
     CDS             complement(465..806)
                     /locus_tag="VEA63_14635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009513739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HYD85299.1"
                     /translation="MNAPADCRRCGVCCFSRAEAYVRVTGEDWARLGEQAEAMAHFIG
                     NRAFMRMAGGHCAALEVRRDADGAADYFCGIYERRPQVCRDLARGSPECAGERETKAE
                     GPGQCGVLVRG"
     gene            1126..1704
                     /locus_tag="VEA63_14640"
     CDS             1126..1704
                     /locus_tag="VEA63_14640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007362509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4202 domain-containing protein"
                     /protein_id="HYD85300.1"
                     /translation="MDAYQRARQLIDVAHAADPNRAADGRPAELVYAERMEAWVARVA
                     ADATPLLRLAARCQHLERWSVPRASFPEGKVGYLTWRRSLYTKQAERARSLLLEAGVS
                     ADEAAEVATWVSKSGLKTNPGTQALEDAACLVFLENEIGAFAAQHAEYPREKFVDIIR
                     KTWRKMSPRAHELARALDLPPGIAALVQEAIA"
     gene            complement(1820..>2226)
                     /locus_tag="VEA63_14645"
     CDS             complement(1820..>2226)
                     /locus_tag="VEA63_14645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009513662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="permease"
                     /protein_id="HYD85301.1"
                     /translation="PLVWHMPTNANHMLREVGITLFLAAVGLRSGDKFVEVLLGADGL
                     RWLMYGAAVTAVPLVIVGLVARFWKKLNYAELCGLLAGSMTDPPALAFAQQTTASDAP
                     AVAYATVYPLVMLLRVFSAQLVVFLLYRAATG"
BASE COUNT          442 a          851 c          592 g          341 t
ORIGIN      
        1 tcgcgtccac gcctttttcc ttcgcgacgg cgacgatgct ctcgatgtcg agatacgccg
       61 cgaccggtcc cttgccttgg ccaacctggt aagcctcgtc ggccttgtag cgatggatgc
      121 tcaaccgatc ctcgtgcgcg aagatcgcca ccgtgcgcaa gccgagctcc gtgcccgcgc
      181 gaaagatgcg gacggcgatc tcgctgcggt tggcagccat gagtttcttg acggggcgaa
      241 tggaggccgc gggggcgggt gcttcagaag ggagcatgtg cggtaaaagg gggaaggcgg
      301 gttttatgac gcgagcggtc gaacgcaacg gaagactgcc cgcgaagcgc atctacgcgc
      361 gagaaacgcc gcggacagtt cagcgcccct agggatgtgg ctcgctgcgc gagcagcggg
      421 aaaacgacaa ccctcggcgc ttgcaatcgc ctgcaagcag gctcctaccc tcgaaccaaa
      481 acgccgcatt ggcccggccc ctccgctttt gtttcccgct cccccgcaca ctccggcgaa
      541 ccgcgcgcca gatcccggca cacctgcggc cgacgctcgt aaatcccaca gaaataatcc
      601 gccgcaccgt ccgcgtcgcg ccgcacctcc agcgctgcgc aatgcccgcc ggccatccgc
      661 atgaacgcgc gattcccgat gaaatgcgcc atcgcttccg cctgctcgcc cagccgcgcc
      721 caatcctcgc ccgtcacgcg cacgtaagct tccgcccgcg aaaaacaaca caccccacaa
      781 cgccgacaat ccgccggagc attcatgttg agatgtggga ggggctttac gccccgactc
      841 gcgcagcatc cgcaaacccg ctgccgctca gggcagaaat cccctcccac agttcccacc
      901 ccaacgcgat ctcattccgg tgagtctgcc acctctccca ccgcggcagc acaaccgacg
      961 atggagggac gacctccgtg tcgtcctcgg accggacgga gccggtccct ccgcctcgac
     1021 caaaatcgtc gaagaagaac gattcgcacc gaccgtttta cctgctccac caccccagca
     1081 ttgcctcacg ccacccgcac cttttgcctc acgcccgcac ttctcatgga cgcctaccaa
     1141 cgagctcgtc aactcatcga cgtcgcgcac gccgccgatc ccaaccgcgc cgccgacgga
     1201 cgtcccgccg aactcgttta cgccgagcgc atggaagcct gggtcgcgcg cgtcgccgct
     1261 gacgccacac cactcctgcg cctcgccgcg cgttgccaac atctcgagcg ctggtccgtg
     1321 ccccgcgcga gctttcccga aggcaaagtc ggctatctca cgtggcgtcg atcgctctac
     1381 accaaacaag ccgaacgcgc gcgctctctc ctcctcgaag ccggcgtatc cgccgacgaa
     1441 gccgccgaag tcgcgacttg ggtctcgaaa agcggcctga aaaccaatcc cggcacccaa
     1501 gcgctcgaag acgccgcgtg cctcgtgttt ttggaaaacg agatcggcgc gttcgccgcg
     1561 cagcacgccg aatatccgcg cgaaaagttc gtcgatatca ttcgcaaaac ctggcgcaaa
     1621 atgagcccgc gcgcccacga actcgctcgc gctctcgacc tcccgcccgg catcgccgcc
     1681 ctcgtgcagg aagcgatcgc ctaagcgagg tagggacgcc tctccgaggc gtccgctttc
     1741 ttggcccatc acggacgtct ccgagagacg tccctaccat taaaaaaccc gacgaggacg
     1801 ttgggttcca ccttccccct catcccgtcg ccgcgcgata caacaagaac accacgagct
     1861 gcgccgaaaa cacccgcagc aacatcacca gcggatacac cgtcgcatac gccaccgccg
     1921 gcgcgtcgct cgcggtcgtc tgctgcgcaa acgccagcgc cggcggatcc gtcatgctgc
     1981 ccgctagcaa cccgcacagc tcggcgtagt tcagcttttt ccaaaaacgc gccaccagcc
     2041 cgacgatcac cagcggcacg gccgtcaccg ccgcgccata catcagccac cgcaaaccgt
     2101 ccgcgccgag cagcacctcc acaaatttgt cgcccgaacg caaacccacc gccgcgagaa
     2161 ataacgtaat cccgacttcg cgcagcatgt gattcgcatt cgtcggcatg tgccacacca
     2221 gcgggc
//
LOCUS       DASPLT010001436         3384 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_98062, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001436 DASPLT010000000
VERSION     DASPLT010001436.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 3384)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3384)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3384
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_98062"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1087)
                     /locus_tag="VEA63_14650"
     CDS             complement(<1..1087)
                     /locus_tag="VEA63_14650"
                     /inference="COORDINATES: protein motif:HMM:NF037015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HYD85302.1"
                     /translation="MKPRFQFFLTALLSVSLALCARAQQQPVVGVDIVQLSVLTGQGS
                     VAYVELSSEGGGYTNAPDVTIEAPAGGGVRATARAVLTVGGTVSRIEIIDPGSGYTSN
                     PEILISGGGGAGAVAFAQVGDVFGRDVVNDSFGSYRDTIAVTAEAVGTFVNSSFTYSF
                     FVNGVSIGETTGPVPPGVRPTVLWRPPQPGFYFITVRATDGVSTVTSLAVRYFAEGTV
                     VNSPLPGLVPQGSSVVVKADATVAGGFIQKIQFYDNGDLIGEDATIPYSIVYPVPGAS
                     GTVHNITARATDNESTAGSMSLPIKLTVVTPITPVATSVISTPANGAVIPIPSTLPIT
                     PIPIIVDATSRSGRISKVEAYVDGELFG"
     gene            complement(1131..2849)
                     /locus_tag="VEA63_14655"
     CDS             complement(1131..2849)
                     /locus_tag="VEA63_14655"
                     /inference="COORDINATES: protein motif:HMM:NF012485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretin N-terminal domain-containing protein"
                     /protein_id="HYD85303.1"
                     /translation="MRTRTFVSLAVLLAVSIAQGQAQIDPATEQPAAAPAITPPPATN
                     GEPEITIKELGTTAGGAVKSRDASGYDTLSVDFPDEDVRNILRNVADLFELNLVIPEE
                     LQGKASIKLRDVTWRQIFEVVLAPVGYTYIEEGNIIKVISNDTLLQEPVATEVFIINY
                     ARAADIMPTITSLVDPAAGGRIVVDARSNSLVITERPSRMNRIRPIIEQLDRATDQVM
                     IESKFVEVTDRDVKNLGVNWASLNAYNIAVGGDEQGVVGGYEREQTAERTNGESGNTN
                     TTGTQGTTSTSANTVTSTNGVTTATSSTGVTSTIGNETATSLDLLNSLVGGSTTSRTL
                     NAVFSADQFGIVLSALQTLGNTKIVSNPTIVTLNNTEASINVGEERPIPNYTFSEQTG
                     TFQISGFTYKPIGVLLKVTPQVNARGMIKLTLSPEVSQTNGNVTFGGASGTQIPIVAT
                     RKATTQVSLKNGYTMGIGGLVTQNATKGETKVPVLGSVPVLGRLFRSDTRESTTSNLI
                     IFITAKSLSAEGAPVEEIFESSRVRQLEMKREDLPGHRDGSDPFVPPVPVEPETKKKS
                     KWFGRK"
     gene            complement(2846..>3384)
                     /locus_tag="VEA63_14660"
     CDS             complement(2846..>3384)
                     /locus_tag="VEA63_14660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD85304.1"
                     /translation="PAMNVSCFRILFLGAGVFAVVPTSHAAALSDLMSSEVRRPVVEL
                     AQRIAKPSAPRPLPEDLVHPFNPAAFGQPDPEELRAIAAAQAAAAASAVKAKPTTNLG
                     LLELIASRINPSGTLILGGEPLLIFGQKRLRVGDRLTVTYDGLDYPLELTAIERTTFT
                     LRLNSDEITRRIQPGKSQ"
BASE COUNT          606 a         1073 c         1038 g          667 t
ORIGIN      
        1 tcccgaagag ttcgccgtcg acgtatgcct cgaccttgct gatgcgtccg ctccgactcg
       61 tcgcatccac gatgatcgga atcggcgtga tcgggagcgt tgatggaatc ggaatcaccg
      121 caccattcgc cggcgtgctg atcacgctcg tcgccaccgg cgtgatcggc gtcaccaccg
      181 tcaacttgat cggaagactc atcgagcctg cggtgctctc gttgtcggtt gcgcgagctg
      241 tgatgttatg aacggtgccg ctggcgccgg gcacggggta aacaatggaa tacgggatcg
      301 tcgcatcctc accgatcaga tcgccgttat cgtagaattg gattttctgg atgaagccac
      361 cggccaccgt cgcatccgct ttcaccacga cggacgaacc ctgcggcacg agtcccggca
      421 gcgggctgtt caccaccgtg ccttccgcga aataccggac ggccagcgat gtgaccgtcg
      481 atacgccgtc ggttgccctc accgtgatga aataaaaacc cggttgtggc ggcctccaca
      541 gcaccgtcgg ccgaactccc ggcggaaccg gaccggtcgt ctcgccgatc gacacgccgt
      601 tcacgaaaaa cgaatacgta aagctcgagt tcacgaacgt gccgaccgcc tccgccgtca
      661 ctgcgatcgt gtcccgatac gaaccgaatg aatcattgac gacatccctg ccaaaaacgt
      721 cgccgacctg ggcaaaggcc accgcgcctg cgccgcctcc gccgctgatg aggatctctg
      781 gattcgaagt atatcccgat cccggatcga tgatttcaat tcgcgaaacc gtgccgccga
      841 ccgtaagaac cgcgcgcgcc gtagcccgca cgccgccccc tgccggcgcc tcgatcgtca
      901 cgtccggcgc attcgtgtag ccgcctcctt cagatgacag ttcaacgtag gcgacgctgc
      961 cttgccccgt cagcacggag agctgcacga tgtcgacgcc gacgaccggc tgctgctgcg
     1021 cccgggcaca cagcgcaaga gagacgctca gcaacgcggt gaggaaaaac tggaatcgcg
     1081 gtttcatgat tacacctcgg attgggttgg cggtgggtgg agccgtgagc tcacttgcga
     1141 ccgaaccact tggatttttt cttcgtctcg ggttccacgg gcaccggcgg cacgaaagga
     1201 tccgaaccgt cgcgatgacc gggcaggtcc tcgcgtttca tctcgagctg gcgcacgcgc
     1261 gacgactcga agatttcctc gactggggcg ccttccgcgc tgagcgattt ggcggtgatg
     1321 aaaatgatca ggttgctcgt cgtgctctcg cgcgtgtcgg agcgaaacag ccgcccgagg
     1381 accggcaccg agccaagcac cggcaccttc gtctcgccct tcgttgcgtt ctgcgtgacg
     1441 aggccgccga tgcccatcgt gtaaccattt ttcaacgaca cctgcgtggt cgccttgcgc
     1501 gtcgctacga tcggaatctg cgtgccgctc gcgccgccga aggtcacgtt gccattcgtc
     1561 tggctcactt ccggcgagag cgtgagcttg atcatgcccc gcgcgttcac ttgcggcgtc
     1621 actttcaaca acacgccgat cggcttgtag gtgaagccgc tgatctgaaa ggtgcccgtc
     1681 tgttcgctga acgtgtaatt cggtatcggc cgttcctcgc cgacgttgat cgatgcttcg
     1741 gtgttgttga gcgtgacgat cgtcggattg gaaacgatct tggtgttgcc caacgtctga
     1801 agcgcactga gcacgatgcc gaattgatcc gcgctgaaaa cggcgttcaa cgtgcggctg
     1861 gtggtggatc cgcccaccag tgagttcaac aagtccagcg acgtcgccgt ttcattgccg
     1921 atcgtgctcg tcaccccggt cgacgaggtc gccgtcgtga cgccgttcgt cgaagtaacc
     1981 gtattggcgg aggtcgaagt ggtgccttgg gtgccagtcg tgttcgtgtt cccgctctcg
     2041 ccgttggtgc gttcagcggt ttgttcgcgc tcgtagcccc cgacgactcc ctgctcatcg
     2101 ccgccgacgg cgatgttgta ggcgttgagc gaggcccagt tgacgccgag gttcttcacg
     2161 tcgcgatccg tcacttcgac gaacttcgac tcgatcatca cctgatccgt cgcgcgatcg
     2221 agttgctcga tgattggacg aatgcgattc atccgcgaag gccgctccgt gatcacaagg
     2281 ctgttgctac gcgcatcgac cacgatccgg ccgccggccg ccggatcgac cagcgacgtg
     2341 atcgtcggca tgatgtccgc ggcgcgcgcg tagttgatga tgaagacctc cgtcgcgacc
     2401 ggctcctgga gcagcgtgtc attcgagatg actttgatga tgttgccttc ctcgatgtaa
     2461 gtgtaaccaa ccggcgcgag aacaacctcg aaaatttgcc gccacgtcac gtcacgcagc
     2521 ttgatcgacg ccttgccctg gagttcctcg ggaatcacga ggttcaactc gaacaggtcg
     2581 gccacattgc ggagaatgtt gcgcacatcc tcgtccggga aatccaccga aagcgtgtcg
     2641 tagccggaag cgtcgcgact cttgaccgcg ccgcccgcgg tcgtgcccag ctccttgatg
     2701 gtgatctccg gctcgccgtt cgtcgccggc ggcggagtga tcgcgggcgc ggccgccggc
     2761 tgctcggtgg ccggatcgat ctgggcttgt ccttgcgcga tggatacggc cagcagcacg
     2821 gcaagcgaaa cgaaggtgcg ggttctcatt gggactttcc tggttgaatg cgccgggtga
     2881 tctcgtcgct gttgagtcga agcgtaaaag tggtgcgctc gatcgccgtc agctcgagcg
     2941 ggtaatccaa gccgtcatac gtgaccgtca ggcggtcccc aacacgcagg cgtttctgcc
     3001 cgaagatgag cagcggttcg ccgccgagga tcagcgtgcc ggaaggattg atgcgcgacg
     3061 cgatgagctc gagcagccca agattggttg tcggtttcgc cttgaccgcc gacgccgccg
     3121 cggcggcctg cgctgcggcg atcgcgcgca gctcttcggg atccggctgg ccgaacgccg
     3181 ccgggttgaa cggatgcacg aggtcctccg ggagcggacg cggcgccgac ggtttcgcga
     3241 tgcgttgcgc aagctccacc acgggacgac gcacctccga cgacatgaga tccgacaacg
     3301 ccgcggcgtg gctcgtcggc accaccgcga aaacgcctgc gcctaaaaag agtatccgaa
     3361 aacaggagac gttcatggct ggcc
//
LOCUS       DASPLT010001437         1236 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_181799, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001437 DASPLT010000000
VERSION     DASPLT010001437.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1236)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1236)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1236
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_181799"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..231
                     /locus_tag="VEA63_14665"
     CDS             <1..231
                     /locus_tag="VEA63_14665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD85305.1"
                     /translation="IQVASAEIANFDYHTGGPITGKRGEFQEVRIPFKDMKRAWSEQT
                     TLNLKTITSVNLVAFGTVRDAFAYEVDEIGFY"
     gene            283..>1236
                     /locus_tag="VEA63_14670"
     CDS             283..>1236
                     /locus_tag="VEA63_14670"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HYD85306.1"
                     /translation="MSPLTENSRLDYLDATRAFALVLGIVFHASLSFMPVFMGWAVMD
                     ISTSPIVASLVLVCHAFRMETFFLLAGFLSHLSFHRHGADEFVRARVLRIVVPFVVGW
                     FILRPLVVSGWIMGGASLRGDYDFGAGILAGFKSLEGLPAGIFTGLHLWFLYYLAMIT
                     ALALGFCWLVKTAGGRAGAMARRGDIIVAWLARSRWGVPVLIVPTAVALWFMRFWGMD
                     TPDQTLNPHVPVLLVYGGFFALGWMLSRQRDALADFVRLSASRWVLAGIGIIGAVWLA
                     AIERDPSHPHYVAAHAGFAVSYALMMWSLVFLTLGVFKKFCA"
BASE COUNT          194 a          338 c          411 g          293 t
ORIGIN      
        1 atccaggttg ccagcgccga gatcgcgaac ttcgactatc acaccggtgg accgatcacg
       61 gggaaacgcg gcgagtttca ggaagtgcgg attcccttca aagatatgaa gcgcgcgtgg
      121 tcggagcaga ccacgctcaa cttgaaaacg atcaccagcg tcaacctcgt cgccttcggc
      181 accgttcgag atgcgttcgc ttacgaggtg gacgagatcg ggttttattg aacggcgaca
      241 tactgaaacg cggaacgaaa tcccgacgaa agccctatcg ccatgtcacc gctcacggaa
      301 aattcgcggc tggattacct cgacgcgacg cgcgccttcg cgctcgtgct cggcatcgtg
      361 tttcacgcca gcttgtcgtt catgccagtg ttcatgggct gggcggtgat ggatatttcg
      421 acgagcccga tcgtcgcgtc gcttgtcctg gtctgccacg cgttccgcat ggaaacgttt
      481 ttcctgcttg cgggattttt aagccatttg agttttcatc gtcacggtgc cgacgaattc
      541 gtgcgcgcca gggtgctgcg gatcgtggtg cccttcgtgg tcggctggtt catcctgcgg
      601 ccgttggtgg tgtcgggctg gatcatgggc ggagcgagtt tgcgcgggga ctacgatttt
      661 ggggcgggaa ttctggccgg gtttaaaagt ctcgaaggtt tgcccgcagg aattttcaca
      721 ggtttgcacc tgtggttcct gtattacctc gcgatgatca ccgcgctggc gcttggattt
      781 tgctggctgg tgaaaacggc aggcgggcga gcgggcgcga tggcgcggcg cggcgacatc
      841 atcgtcgctt ggctggcgcg atcgcggtgg ggtgtgccgg tgctgatcgt gcctaccgcg
      901 gtcgcgctgt ggttcatgcg gttctggggg atggacacgc cggatcaaac gctgaacccg
      961 cacgtgcccg tgcttctggt gtatggcggt ttcttcgcgc tcggatggat gttgagccgg
     1021 caacgcgatg cgctcgcgga tttcgtgcgg ttgtcggctt cgcgctgggt tctcgcgggg
     1081 atcggcatca tcggggcggt ttggctggcg gcgatcgagc gcgacccgag ccatccgcat
     1141 tacgtcgcgg cgcatgccgg cttcgccgtg agttacgcac tgatgatgtg gtcgctcgtt
     1201 tttctcaccc tcggcgtgtt taaaaaattc tgcgcc
//
LOCUS       DASPLT010001438         4768 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_221707, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001438 DASPLT010000000
VERSION     DASPLT010001438.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 4768)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4768)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4768
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_221707"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..226
                     /locus_tag="VEA63_14675"
     CDS             <1..226
                     /locus_tag="VEA63_14675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD85307.1"
                     /translation="ARAAAASKRAASRTPQIMEASRVCANACRACGRALGTAFREAQA
                     AADAAAAKILYGSLRALEKQMWVLDPRQAD"
     gene            483..2672
                     /locus_tag="VEA63_14680"
     CDS             483..2672
                     /locus_tag="VEA63_14680"
                     /inference="COORDINATES: protein motif:HMM:NF017077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HYD85308.1"
                     /translation="MRLLNPRIYTYLVGLWIALSLAGLGLGVVVWKNLSNSFEATVDS
                     AQFRRSLRDVFSALQDAETSERGFLLSGNESYLEPFNRADAELPAYFEELAADAKEQP
                     DLLDDVLTLKGLVELKLASLRQAIESRRQSTWSTTFNRARQEESRAMMERIRATIERM
                     DRRPQDLVNASGAATRLRIQQALLATLLAGVLGLGAGMIALYLSRLALRQEKNERLLA
                     EQAIRAESAAREKSAFLANMSHEIRTPMNAIVGFSELLVADVPPTSKARQRVQAIRDS
                     AASLLQLINDILDLSKIDAGAVELHIEPTDLREVGAFMHTVFAQQAARKALVLTCEVD
                     AAVPAVLLLDRSRIRQILVNLLGNAVKFTERGEVALRVTWEVDPAKPASGTLQLTVRD
                     TGIGIPAEKLKDIYRPFVQVNPQRQSERQGSGLGLSIVQRLAQRLGGEIAVESELGHG
                     SCFCVRLPNVAVSARLPDHARADRNERVDFADLQPAELLVVDDDAASRELLSGYFEQT
                     PHQLRFAANGREALELVREKRPDIVLMDIRMPEMDGRTALTELGKLPGAELLPVIAVT
                     AANMMDDEQVLRGRFAGYLRKPLTRQALFQELAAFLPRTPSRSPFPMPSPSSPEPTAA
                     GGTASWPQLLPTLRQLEATLWREASDSGAVSDVKALARRLHELGRSAACAPLAEYASA
                     LEREVDDYAIVRMEARLAEFPRLILALAAAAPSVAAPTTLSSSSPSS"
     gene            2674..3783
                     /locus_tag="VEA63_14685"
     CDS             2674..3783
                     /locus_tag="VEA63_14685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hybrid sensor histidine kinase/response
                     regulator"
                     /protein_id="HYD85309.1"
                     /translation="MTRETPSARILVVDDQETNLRVLGDMLGHLGFDIVPALDGDQAL
                     RRLAARPVDLILLDVLMPGLDGFEVCKRIRANVAWIDIPIIFLSAADDKTLIVRALES
                     GGVDYVTKPFNAAELLSRVRTHLALKTARDRLRQLAEDKDELLGILAHDLKNHLGGML
                     MSVQLLNEKAKSINDARFQRMGANILRATDQMLSFVKEFLANSEVDHGRPMKFQPVYL
                     HDVASNIVQRHSEHAQRKGITLELEQREEGVLVEGDRAALEQVLDNLVSNALKFSPSG
                     KTVRLVVGLAADGRGECRVIDEGPGFTEHDKQQIFRRYRRLSARPTGGEPSTGLGLSI
                     VKKLMQDMRGDLTFESVAGNGATFILTFSPAAVNA"
     gene            3867..>4768
                     /locus_tag="VEA63_14690"
     CDS             3867..>4768
                     /locus_tag="VEA63_14690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HYD85310.1"
                     /translation="MDFLVVDDDQIFREATCLLIEEEGHFATSASNGDVALERVREDK
                     FDAVLLDLHLGRQNGLTLLPMLLKVRPNLPIVMFSAQGTVKTAVQALHQGAVDFLEKP
                     FTRDQFHAVLARLRRISEMGRKIERLEEQVKEVQSQSPEPLIDFETPVMRAVMDVLLR
                     AAKSNASVLILGESGTGKSVAAKALHAQSHLSERPFVTVSCPALSKELLESQLFGHVR
                     GSFTGALRDHWGKVKAAEGGTLFLDEIGDLPLEIQPKLLRLLQEKEYERVGETVTRRS
                     DVRIVAATNRDLKKRVAEGAFREDL"
BASE COUNT          830 a         1483 c         1586 g          869 t
ORIGIN      
        1 cgcgcgcgcc gcggccgctt cgaagcgcgc ggcatcgcgc actccgcaga tcatggaagc
       61 gtcgcgcgtc tgcgcaaacg cctgccgggc ttgcggccgt gcgctcggca ctgcgtttcg
      121 cgaagcgcaa gccgcggcgg acgctgcggc ggcgaagatc ctctacggct ctttgcgtgc
      181 gctggaaaaa cagatgtggg tgctcgatcc gcgtcaggca gattaacccc agcggcagtc
      241 ttccgcgcca tttgcgcata gcggcaggcc tccgcgcctg cctgctttgc tcggcttttt
      301 tcgtgaaagg tgtgtggcgc gctggcgcac cacattgcgc gcgagcgcgc aacccacaac
      361 aaggcgcgaa ggccggcatc ggggaaagtc ccgatgtgtt tcggccggtt gacattttgg
      421 cgtttagcgt cccgctcttg agcccggagc gcccgtcgaa acggctgccc cggtgttcga
      481 tcatgcgcct gctcaacccg cgaatctaca cctatcttgt cggactctgg atcgcgttgt
      541 cgctcgccgg cctcgggctc ggcgtggtgg tgtggaaaaa cctgagcaac agcttcgagg
      601 cgaccgtcga ctcggcgcag tttcgccgct cgctgcgcga tgtgttttcc gcgttgcagg
      661 acgccgagac cagcgagcgc ggctttctcc tcagcggcaa cgagagttat ctcgagccgt
      721 tcaaccgtgc cgacgccgaa ctgcccgcgt atttcgagga actcgccgcc gacgcgaagg
      781 aacagcctga tctgctcgac gacgtcctca cgctcaaggg tctcgtcgaa ctcaagctcg
      841 cgtcgctccg ccaagccatc gagtcgcggc ggcaatccac gtggtcgacc accttcaacc
      901 gcgcgcgaca ggaagagagc cgcgcgatga tggagcggat ccgcgccacc atcgaacgca
      961 tggatcgacg cccgcaggat ctcgtcaatg cctccggcgc cgcgacgcgc ctgcgcattc
     1021 agcaggcgtt gctcgccacg ttgctcgccg gcgtgctcgg gctcggcgcc ggcatgatcg
     1081 cgctttatct ttcgcgactc gcgttgagac aggagaaaaa cgagcgcctt ctcgccgagc
     1141 aggcgatccg cgccgagagc gctgcgcgcg agaaaagcgc gttcctcgcg aatatgagcc
     1201 acgagattcg cacgccgatg aatgcgatcg tcggcttcag cgagctgctc gtcgccgacg
     1261 tgccgcccac cagcaaggct cgtcaacgcg tgcaagcgat ccgcgacagc gctgcgtcgt
     1321 tgctccagct gatcaacgac atcctcgatc tttcgaaaat cgacgcgggc gcggtcgagc
     1381 tgcacatcga gccgacggac ctgcgcgaag tcggcgcgtt catgcacacg gtttttgcgc
     1441 agcaggccgc gcgcaaagcg ctggtgctta cctgtgaagt cgacgctgcg gtgccggccg
     1501 tgctgctgct cgaccgttcg cgcatccggc agattttggt caacctcctc ggcaacgcgg
     1561 tgaagttcac ggagcgcggc gaggtcgcgt tgcgcgtcac gtgggaagtc gatcccgcga
     1621 aacccgccag cggcacgttg caactgacgg tgcgcgacac gggcatcggc atcccggcgg
     1681 agaagttgaa ggacatctat cggcccttcg tgcaggtgaa tccgcagcgg cagtctgagc
     1741 gacagggttc ggggctcggc ctgagcatcg tgcaacgtct cgcgcaacgt ctcggcggcg
     1801 aaatcgccgt cgagagcgag cttgggcacg gttcatgttt ttgcgtgcgt ttaccgaacg
     1861 tggcggtgtc ggcgcgtttg cccgaccatg cgcgcgccga tcgcaatgaa cgcgtggatt
     1921 tcgccgactt gcagccggcc gagttgctgg tggtcgatga cgatgccgcg agccgcgagc
     1981 tgctctcggg atatttcgag caaacgccgc accagttgcg cttcgccgcg aatggtcgcg
     2041 aagcgctcga gctcgtgcgg gagaaacgtc cggacatcgt tctgatggat attcgcatgc
     2101 ctgaaatgga cgggcgcacc gcgttgacgg agctcggcaa gctacctggc gccgaacttc
     2161 tcccggtgat tgcggtcacg gctgcgaaca tgatggatga cgaacaggtt ttgcggggac
     2221 ggtttgcggg ctatctgcga aaaccactca cgcgacaggc gttgtttcag gagctcgcgg
     2281 cgtttttgcc gcgcacgcct tcgcgatcgc cgttcccgat gccatcgccg tcttcgcccg
     2341 agccaacggc ggcgggcggc accgcgagct ggccgcaatt gttgcccacg ctccggcaac
     2401 tcgaagccac gttgtggcgc gaggcgtccg acagcggcgc ggtcagcgac gtgaaagcgc
     2461 tcgcccgccg gctccacgaa ctcggtcgct cggcggcgtg cgcgccactc gcggagtatg
     2521 cgagcgcgct ggaacgcgag gtcgacgatt acgcgatcgt gcgcatggaa gctcgcctcg
     2581 cggaatttcc gcgtttgatt ttggcgctcg ccgcggccgc accgagcgtc gctgcgccga
     2641 cgactttatc ctcctcttcc ccgtcctcct gatatgactc gcgaaacgcc gtccgcacgc
     2701 atcctggtag tcgacgatca agagaccaat cttcgggtgc tgggcgacat gctcggtcat
     2761 ctcggcttcg acatcgtgcc cgcgctcgac ggcgatcaag cgttgcgccg cctcgctgcg
     2821 cggcccgtcg acctgatcct gctcgacgtg ctcatgccgg ggctcgacgg cttcgaggtg
     2881 tgcaaacgca tccgcgcgaa cgtcgcgtgg atcgacatcc cgatcatctt tctgtccgcc
     2941 gccgacgaca aaacgctcat cgtgcgcgcg ctggaaagcg gcggcgtcga ctacgtgacg
     3001 aagccgttca acgccgccga gttgttgtcg cgcgtgcgca cgcacctcgc attgaagacc
     3061 gctcgcgatc ggctgcgtca gctcgccgag gacaaggacg agctgctcgg cattctcgcg
     3121 cacgatttga agaatcactt gggcgggatg ctgatgagcg tgcagttgct caacgagaag
     3181 gcgaagtcga tcaacgatgc gcggtttcaa cgcatgggtg cgaacatcct gcgcgcaacg
     3241 gaccagatgc tttcgttcgt gaaggagttt ctcgcgaact ccgaggtcga tcatggccgg
     3301 ccgatgaaat tccagccggt ttatctgcac gacgtcgcga gcaacatcgt gcaacggcac
     3361 agcgaacatg cgcagcgcaa gggcatcacg ctcgagctcg agcagcgcga agaaggcgtg
     3421 ctcgtcgaag gcgatcgcgc ggcgctcgag caggttctgg acaatctcgt gtcgaacgcg
     3481 ctgaagtttt cgccctccgg caaaacggtg cggctcgtcg tcgggctcgc ggccgacgga
     3541 cgcggcgagt gtcgcgtgat cgacgaaggc ccgggtttca ccgagcacga caagcagcag
     3601 atcttccggc gttaccggcg gctgagcgcg cggccgacgg gtggcgaacc ttcgacgggg
     3661 ctcggcctca gcatcgttaa aaaactgatg caggacatgc gcggcgattt gaccttcgag
     3721 agcgtcgcgg ggaacggcgc gacgtttatc ctgacgttct cgcccgcggc ggtgaacgcg
     3781 tgaaaccagc gcacgccatg aaacgatttt ccggaggctc ctaccttccg acgatccgtt
     3841 tcgtcataac gttggtctga ttcgccatgg attttctcgt agtcgacgac gatcagattt
     3901 tccgcgaggc gacgtgcctg ctcatcgaag aggagggtca cttcgcgaca agtgcgtcga
     3961 atggcgacgt agcgctcgag cgcgtgcgcg aggacaagtt cgacgcggtg ctgctcgatc
     4021 tgcacctcgg ccggcaaaac gggctgacgc tgctgccgat gctgttgaag gtgcgtccga
     4081 acctgccgat cgtgatgttc tccgcgcaag gcacggtgaa gacggcggtg caggcgttgc
     4141 accaaggcgc ggtcgatttt ctggagaagc cgttcacgcg cgaccagttt cacgcggtgc
     4201 tcgcgcggtt gcgccgtatc agcgaaatgg gccgcaagat cgagcggctc gaggagcagg
     4261 tgaaggaagt gcagtcgcag agcccggagc cgctgatcga tttcgagacg ccggtgatgc
     4321 gcgcggtgat ggacgtgttg ttgcgggcgg cgaagagcaa cgcgtcggtg ttgatcctcg
     4381 gcgagagcgg cacgggcaag agcgtcgcgg cgaaagcgct gcatgcgcag agccacctga
     4441 gcgagcggcc gtttgtgacg gtgagctgtc cggcgttgtc gaaggagttg ctcgagagtc
     4501 agctctttgg ccacgtgcgc ggttcgttca ccggcgcgct gcgcgatcac tggggcaagg
     4561 tgaaggccgc ggaaggcggc acgttgttcc tcgacgaaat cggcgatctg ccgctggaga
     4621 ttcagccgaa gttgctgcgc ctgttgcagg aaaaggaata cgaacgcgtc ggtgaaacgg
     4681 tgacgcgtcg gtcggacgtg cgcatcgtgg cggcgacgaa tcgcgatttg aagaagcgcg
     4741 tggccgaggg cgcgtttcgc gaggacct
//
LOCUS       DASPLT010001439         1853 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_56647, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001439 DASPLT010000000
VERSION     DASPLT010001439.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1853)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1853)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1853
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_56647"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..734
                     /locus_tag="VEA63_14695"
     CDS             <1..734
                     /locus_tag="VEA63_14695"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HYD85311.1"
                     /translation="LAVFAAVVWAVTFRLIPPVRGHLQSGANKGQAFRALLRDANAGR
                     AILFMALMVLGHFSIIPLLSPHLVGDLALPEKYLSLVYFIGGVASVLTAPRVGRIADR
                     HGRQRVFTYMVVAASVITLTITNAGPLPVWTILVLAGLFFIFASGRFVPGQAIVTLAV
                     PSSRRGAFLSLTSCARDLASGISSTLGGWVVVKQPSGQLLHFNWLGWIAVAAGLLSIA
                     VARTVRVNDTGAPSPLVDLKGEPQR"
     gene            1477..>1853
                     /locus_tag="VEA63_14700"
     CDS             1477..>1853
                     /locus_tag="VEA63_14700"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HYD85312.1"
                     /translation="MKKIGFLSFGHWTPSSQSQTRSAADVLLQSIELAVAAERIGMDG
                     AYFRVHHFARQLASPFPLLAAVGARTKKIEIGTAVIDMRYENPHYMFEDAGSADLIAG
                     SRLQLGISRGSPEQVIDGWRHFG"
BASE COUNT          339 a          546 c          562 g          406 t
ORIGIN      
        1 tcctcgcggt gtttgccgcg gtggtgtggg ccgtgacgtt tcgcttgatc cctccggtgc
       61 gcggacacct ccagagcggc gccaacaagg gccaggcgtt tcgagctttg ttgcgcgacg
      121 ccaatgcggg gcgcgcgatc cttttcatgg cgctcatggt gctcggccat ttcagcatca
      181 ttcccctgct ctctccgcat ctggtcggcg atctggccct accggagaaa tatctttctc
      241 tggtctattt catcggcggc gtcgcgagcg tcctgaccgc gccgcgcgtg ggccggatcg
      301 cggatcgcca cgggcgccag cgcgtcttca cttacatggt ggtcgcggcg tcggtgatca
      361 cgctcacgat cacgaacgcc ggcccgctgc cggtctggac gatcttggtg ctggcgggct
      421 tgttttttat tttcgcgagc gggcgcttcg ttcccggtca ggcgatcgtg acgctcgccg
      481 tgccgtcgtc gaggcgcggg gctttcttga gcctgacgag ttgtgcgcgc gatctggcgt
      541 cgggcatcag ttcgacgctg ggcggctggg tcgtggtcaa acagccgagc gggcagttgc
      601 ttcatttcaa ctggctcggc tggatcgccg tcgcagcggg gctgctgagc atcgcagtcg
      661 cgcgcacggt ccgggtgaac gacaccggcg caccttcgcc gttggtcgat ctcaagggag
      721 aaccgcaacg gtgaaataaa cgcacggccg acgagtcgca gatgagttta ctttgagccg
      781 agcaattcat ttgaaccacg aatgaacacg aagcgacacg aatggaaacc gaccgagcag
      841 gtgatcgcca agaagccaag gttcactggt cgcgcgaaag cctcatgtat ctttcgccgg
      901 agtcgtctgg gattcgtgtg aattcgtatc cattcgtgat gtccgagccg tagccatctt
      961 ggtgaaccac ggaggcgcgg agacacggag aaatctccgg gccttctttg tgttctccgt
     1021 gcctccgtgg ttcaaattgg ttttcgagaa tttccgcgcg agaaaccggc cgctggaaaa
     1081 tcctgactca gaattgaaca gaagataacc aagggaacaa agagcaccga gtggtcgtgc
     1141 gactcttcgt ttccttcgtt accttctata aacaggattg aattctgtgg tttggctgcg
     1201 gcttggccgc gctgcgtaac gggggcctcg attgaattcg ctatgattat cagccgaggc
     1261 gctctcgacg ctcggccgcc ggcggcattc attgcgaatc gctcttccct gcctaccact
     1321 tagccggaac aatgcagtcg agagtccaga gcttagagtc cagagccaga aaaaggtgga
     1381 tcgaaaaatc tgcccggcag gcggcgcaag ccgccggtga tcaagctctc agctctcaac
     1441 tctcagctgt cgactgaata gatcccactt agcgccatga agaaaatcgg tttcctgtcc
     1501 ttcggccatt ggacgccctc gtcacaatca caaacgcgct cagcggcgga tgtgctcttg
     1561 cagtccatcg aactcgcggt cgcggccgag cgcatcggaa tggatggagc ctattttcga
     1621 gttcatcact tcgcccggca actggcttca ccgtttccgc tgctcgcggc ggtcggcgcc
     1681 cgcacgaaga aaatcgagat cggcaccgcg gtcatcgaca tgcggtatga gaatccgcac
     1741 tacatgttcg aagacgccgg ctccgccgat ctgatcgcgg gttcgcggtt gcaactcggc
     1801 atcagccgcg gctctcccga gcaggtgatc gatggctggc gtcactttgg tta
//
LOCUS       DASPLT010001440         1140 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_160209, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001440 DASPLT010000000
VERSION     DASPLT010001440.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1140)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1140)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1140
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_160209"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..419
                     /locus_tag="VEA63_14705"
     CDS             <1..419
                     /locus_tag="VEA63_14705"
                     /inference="COORDINATES: protein motif:HMM:NF013767.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HYD85313.1"
                     /translation="KPGETPVEQVADERVLPKVDPSVLDLNVLEALRSVPGRNGPNLV
                     PELVKAFCCEEPQRHAELERLAAGRESRALAHAAHAFAGSSAVIGAHNSRRALLALER
                     AARGEDWDEVAQRLREVHEAGERLRAAFTSLEEIFW"
     gene            complement(481..978)
                     /locus_tag="VEA63_14710"
     CDS             complement(481..978)
                     /locus_tag="VEA63_14710"
                     /inference="COORDINATES: protein motif:HMM:NF013540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HYD85314.1"
                     /translation="MDYTQLFRTLREAKDLTLEELAALARVHRNTVVNIESGRPVKFK
                     TIATLMRKMGYPQSSPEMKSMALLWLESVSGISFSRPETASAAKKNITAHRGAHRRAA
                     EQLEEELTRSRLSAEQIELLVFAAQRREIMSILESIRGLVESGAPAQPLALKAAEEQE
                     PYSGR"
BASE COUNT          207 a          317 c          398 g          218 t
ORIGIN      
        1 tgaagccggg ggaaactccg gtcgagcaag tggcagacga acgcgtgctg ccgaaggtcg
       61 atcccagcgt gctggatctg aacgtgctgg aggccttgcg gagcgtgccg ggaaggaacg
      121 gcccgaatct cgtgccggag ctggtaaagg cgttttgttg tgaagaacca cagcggcacg
      181 cggaacttga gcgcttggcg gcggggcgag aaagtcgcgc gctcgcgcac gcggcgcatg
      241 cgttcgccgg cagttcggcg gtgatcggtg cgcacaactc gcgtcgcgcg ctgctggccc
      301 ttgaacgcgc cgcgcggggc gaagattggg acgaggttgc ccagcgcctt cgtgaagtgc
      361 acgaagccgg cgaacggctg cgggcggcgt tcacgtcgct tgaagagatc ttctggtgag
      421 cggcgtcgcg gcgggtgcga atggtccgtc cgagtgggct ttgccgtggg cgacgagagt
      481 tcaacggccg ctgtaaggtt cttgttcttc ggcggctttt agcgcgagcg gctgagcagg
      541 agcaccgctc tccacgaggc cgcgaatact ctcgagaatg gacatgatct cacggcgctg
      601 cgccgcgaag acgagcagtt cgatctgttc ggccgaaaga cgcgagcgcg tgagctcctc
      661 ttccaattgc tccgcggccc gccgatgcgc tccgcggtgg gcggtgatgt ttttcttcgc
      721 cgctgatgct gtttccggac gagagaagga gatgccgctg accgattcga gccagagcag
      781 cgccatgctt ttcatctcag gtgagctttg cgggtagccc attttgcgca tcagcgtggc
      841 gatggttttg aatttcaccg gtcgccccga ctcgatgttc accacggtgt tccgatgcac
      901 gcgagcgagc gcggcgagct cctccagcgt gagatccttg gcttcgcgga gcgtccggaa
      961 aagctgggtg taatccatgt gcaggcacta gccgcggacc gtggcggatg tcaatgagcc
     1021 ggggcggcac cctgtgatag cgggcatggt tgacaaaaaa tagtctaata caaaaactgt
     1081 gcatgtgtct cgatccgcca agattaaacc tgcgggaaaa cgtcgaccga cgcggcgaat
//
LOCUS       DASPLT010001441         2431 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_241531, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001441 DASPLT010000000
VERSION     DASPLT010001441.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 2431)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2431)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2431
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_241531"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1292
                     /locus_tag="VEA63_14715"
     CDS             <1..1292
                     /locus_tag="VEA63_14715"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HYD85315.1"
                     /translation="FPEMFLHSANFGVAAIDADIFTLAPLNLTLTRNGDTLTGTFAPG
                     KLPVELHRGGTFSDEIDPPDYPAAPPPAWTRDLGAPAWASPATRDGVVYTGTTDGKFH
                     AVAADTGRPLWTWSGDQPLYGEALITNESVCFLDDANHLVSLARADGTLQWRTPLRDP
                     DAPSDSAIPPRKNETFNHRAASPIVDPKGTTLYIGANDGGVYAVRATNGRILWRHDAR
                     APIYAPLLLRGNDLIVGCFDGSVFTLNTRRRRESARTKLGGAIVSAPVVAGNRLVIGS
                     RDYLLYGLDAATHAVAWKNSFWFSWVESTPRLVDGLLYIGGSDFRRVSAIDPATGRRK
                     WATDVRGLSWGSPIVAATTVYAGTTGQNIAGTVIQHTGSLVALDRETGAPRWRYLSPV
                     ATNTDFVGFTGSLVLAGDHIIGAQVDGTLIAFPIANR"
     gene            1638..2423
                     /locus_tag="VEA63_14720"
     CDS             1638..2423
                     /locus_tag="VEA63_14720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD85316.1"
                     /translation="MNKSLVAALLAVVLPLPVVAQDTVAPAPKPAVLKLGVGFDYSHG
                     DYGFSQDTEVSSVPVNLSYDQNRWSFKASIPYLTIKGPASVAAGTTPIAAPGRPTTNS
                     ESGMGDATVSATYHANPKPGQLNVDFTTRVKLPTADEDKGLGTGETDYYFQADLYQAF
                     GDIIPFASFGYRMMGTSAMYPLEDGFYVTIGSSYRLTQKTVIGAAYDWRAKIIEGAEN
                     GTDMIAFIATNPNPNWSLLAYTLIGFNDASPELGLGGLATYKF"
BASE COUNT          445 a          940 c          608 g          438 t
ORIGIN      
        1 acttccccga aatgtttctc cacagcgcga acttcggcgt cgctgcgatc gatgccgaca
       61 tcttcacgct cgcgccgctc aacctcaccc ttacgcgcaa cggcgacaca ctcaccggca
      121 cgttcgcgcc cggaaaactt cccgtcgaac tccaccgtgg cggcacgttc tccgacgaaa
      181 tcgacccgcc cgattatccc gccgcgcctc cgcccgcgtg gacgcgcgat ctcggcgccc
      241 ccgcttgggc ctcgcccgcc acacgcgacg gcgtcgttta caccggcacc accgacggaa
      301 aatttcacgc cgtcgccgcc gacaccggtc gcccgctctg gacctggagc ggcgatcaac
      361 cgctctacgg cgaagcgctg atcacgaacg aaagcgtctg tttcctcgac gacgccaacc
      421 acctcgtttc gctcgcccgc gccgatggca cgttgcaatg gcgcacaccg ctccgcgatc
      481 ccgacgcacc gtccgactcc gcgattccgc cgcgcaaaaa cgaaaccttc aaccaccgcg
      541 ccgcttctcc gatcgtcgac ccaaaaggca cgaccctcta catcggcgcg aacgacggcg
      601 gcgtctacgc cgtgcgcgcc accaacggcc gcatcctctg gcgccacgac gcccgcgctc
      661 cgatatacgc cccgctcctc ctccgtggaa acgacctgat tgtcggttgc ttcgatggca
      721 gtgtgttcac gctcaacacc cgccgacgcc gcgaatccgc gcgcacgaaa ctcggtggcg
      781 ccatcgtctc cgcgccggtc gtcgcgggaa accgcctcgt catcgggtcg cgcgattatc
      841 tcctctacgg cctcgatgcc gccacgcacg ccgtcgcgtg gaaaaactcg ttctggtttt
      901 cctgggtcga atccacgcca cgcctcgtcg atggcctgct ctatatcggc ggctccgatt
      961 ttcgccgcgt cagcgcgatc gatccagcca ccggccgccg taaatgggca accgatgtcc
     1021 gcggactttc gtggggttcg cccatcgtgg ccgcgaccac cgtctacgcc ggcaccaccg
     1081 gtcagaatat cgcgggcaca gtgattcaac acaccggcag cctcgtcgcg ctcgatcgcg
     1141 aaaccggcgc acctcgttgg cgctacctgt cgccggtcgc caccaacaca gacttcgtcg
     1201 gtttcactgg ttcgctcgtc ctcgccggcg atcacatcat cggcgcccaa gtcgacggca
     1261 cgctcatcgc attccccata gccaaccgct aatcagcgcc agcctccgct aatcgatccc
     1321 gaagaacatc cgcgcttcgc cgatcaggcg aagcacgcac gctttcgctt gccccaccgt
     1381 cctccggaaa tcagcgcaga ttagcggtta aaaaactccg acctccgcag ccgttcggtt
     1441 ctcccgcctc gtccgccccg ttaggatttt ttactacgtt ctctttaggg cgatttcgct
     1501 cgggcgtcga accacccgac gcgcctcacc aactatccgt cgcaccgcga tctcccgctc
     1561 gggaacttcg cccgccgctg gctgacgctt ccagggcgca acccgtttcc cgacctgcgt
     1621 ttcccctcac tcccccaatg aacaaatcac tcgtcgccgc cctcctcgct gttgtccttc
     1681 ccctccccgt cgtcgcccaa gacaccgtcg cgcccgcgcc gaaacccgcc gtgctgaaac
     1741 tcggcgtcgg cttcgattat agccacggcg attatggttt ttctcaggac accgaagtct
     1801 cctccgtgcc ggtgaatctt tcctacgatc agaaccgctg gtcgttcaaa gcgagcatcc
     1861 cttacctcac catcaaaggc cccgcgagcg tcgccgccgg cacaaccccg atcgccgcgc
     1921 ccggccgccc gacgacgaat tccgaatccg gcatgggcga cgccaccgtg tccgccacct
     1981 atcacgcgaa ccccaaaccc ggtcagctca acgtcgattt caccacgcgc gtaaaacttc
     2041 caaccgccga cgaggacaaa ggcctcggca ccggcgaaac cgattactac tttcaagccg
     2101 acctctacca agcgttcggc gacatcattc ctttcgcgag cttcggctat cgcatgatgg
     2161 gcaccagcgc gatgtatccg ctcgaagatg gcttttatgt gacgatcggt tcgagttatc
     2221 gcctcacgca aaagactgtg atcggcgccg cctacgactg gcgcgcgaaa atcatcgagg
     2281 gcgccgaaaa cggcaccgac atgatcgcgt tcatcgcgac aaatccgaac cccaactgga
     2341 gcctgctcgc ctacaccctg atcggcttca acgacgccag cccggaactc ggcctcggcg
     2401 gcttggcgac gtataagttc tagccagctt g
//
LOCUS       DASPLT010001442         1202 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_263160, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001442 DASPLT010000000
VERSION     DASPLT010001442.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1202)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1202)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1202
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_263160"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..184)
                     /locus_tag="VEA63_14725"
     CDS             complement(<1..184)
                     /locus_tag="VEA63_14725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD85317.1"
                     /translation="MIFDGRGAPIAARERPEPAEWRDDAITGSCLGHSTVLMNFLGVR
                     VLTDPVFSRRVGPGVSP"
     gene            425..607
                     /locus_tag="VEA63_14730"
     CDS             425..607
                     /locus_tag="VEA63_14730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD85318.1"
                     /translation="MAKKGSKKSYSSKQKRQASHIAAGYKKRGTSTATAKKRAWATVN
                     KTTGGGKKSGSGRKKK"
     gene            657..1070
                     /locus_tag="VEA63_14735"
     CDS             657..1070
                     /locus_tag="VEA63_14735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD85319.1"
                     /translation="MKPLLPALVIALLTPIASAAQASGRPQARPRLQPMPAGTAIRIM
                     ETPAPAPNAEPGAAVALEKMTVQSSKLPSPPRPLEEPPPDSFSFTKGGLLHRKKFSGA
                     TVELSLRRWTDLLAEDARFKPPPTHPKLEVLRVKW"
BASE COUNT          253 a          442 c          333 g          174 t
ORIGIN      
        1 acggcgacac gcccggtccc acgcgccgcg aaaacaccgg atcggtgagc acgcgcacgc
       61 cgagaaaatt catcagcacc gtcgagtgac cgagacaact cccggtaatc gcgtcgtcac
      121 gccactcagc gggctccgga cgctcgcgcg cagcgatcgg cgccccgcgc ccatcgaaaa
      181 tcatccgtgc cacaaatcca agtcgcccca tgtggccgcc aaagagccac ggaaccgctc
      241 gcgcgcaact catcccgtcg caatgccaca cccgagttaa ggaacgtatc gctcgcgcgg
      301 accggattcc ccgcgagctt cgagctccaa tgtccgcgtc cggaaccgtc cgccgctggg
      361 cgaatctatc atcgcgtcag cgtatcagtt ccaccagcac gcgcaccctc aaccccgcac
      421 ccccatggcc aaaaaaggct ccaaaaaaag ttactcgtcg aagcagaaac gtcaggccag
      481 ccacatcgcc gccggctaca agaaacgcgg cacctccacc gccactgcga aaaaacgcgc
      541 ctgggccacc gtcaacaaaa ccaccggcgg cgggaaaaag tccggctcgg gtcgaaagaa
      601 aaaatagcgt ctgcgaagct cgctttgcgc gcgcgttcac cccacgctcg gcgccgatga
      661 aaccgctgct gccggcactc gtcatcgcgc ttctcacgcc gatcgcttcc gcggcgcagg
      721 ccagcggcag accgcaggcc cgcccgcgtc ttcagcccat gcccgccggc accgccattc
      781 gcatcatgga aacacccgcg ccggcgccta atgccgaacc cggcgcggcg gtcgcactcg
      841 aaaagatgac cgtgcaaagc tcaaagttgc cgtcgccgcc tcgccccctc gaggagcctc
      901 cgcccgattc tttttcgttc accaaaggcg gcctgcttca tcggaagaaa ttcagcggcg
      961 cgaccgttga gctgagcttg cggcggtgga ccgatcttct cgccgaggac gcgagattca
     1021 agccgccgcc cacccatccc aaactcgaag ttcttcgcgt caagtggtga gccagcgatg
     1081 cagcgccatc gccaagcgga tgggcgtccc ggttggttga aggtggagcc cgatgtcccc
     1141 atcagactgc tgaactgctg tgaggtaggg cgaaccgtcc tcggtgagcc gttgcgctcg
     1201 cg
//
LOCUS       DASPLT010001443         1130 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_118934, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001443 DASPLT010000000
VERSION     DASPLT010001443.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1130)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1130)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1130
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_118934"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(230..1057)
                     /gene="nadC"
                     /locus_tag="VEA63_14740"
     CDS             complement(230..1057)
                     /gene="nadC"
                     /locus_tag="VEA63_14740"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017404447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HYD85320.1"
                     /translation="MFAGLAQTVAAALAEDIGSGDITAALVPENQMAQARVMCREHAV
                     ICGEAWVDEVFRQVSPAITVQWQRRDGDRVRPEDSIFVASGPARALLTAERTALNFLQ
                     TLSGVATAAREYADLVMHTNVKILDTRKTLPGLRLAQKYAVRTGGCHNHRLGLYDAFL
                     IKENHIAACGSIAAAIATARKQHADRPVEVEVETLAQLEEALTARADIVMLDNFTLEQ
                     TRSAVVLNEGRAKLEASGGFTTESLVAVAETGVDYISVGSLTKHVRATDFTMLFTGA"
BASE COUNT          215 a          337 c          364 g          214 t
ORIGIN      
        1 actggccggt gatgatgcgc ggctccttcg gcagttcctc gtcgaaaccg atcgtgaacg
       61 cttccggcgg cagctccgag cgcggtatct gctgcgcggc gacgaacggc acgaacgcgg
      121 caacggtgac accagcgagc atggacacac acaaacggcg aagacgcgac agcatgagct
      181 ccctccgggg aaatatttct gcgggtgcgg cgcgtgttcc catcagggtt caggcgccgg
      241 tgaacaacat cgtgaaatcg gtggcgcgta catgtttcgt caacgagccg actgatatgt
      301 agtcgacgcc ggtctcggca acggcaacga gcgactcggt cgtgaaaccg cccgacgctt
      361 cgagctttgc gcggccctcg ttcagcacca ccgcgctacg cgtctgctcg agagtgaaat
      421 tgtcgagcat gacgatgtcg gcacgcgcag tgagcgcttc ttccagttgc gcgagcgttt
      481 ccacttccac ttcgaccggc cggtcggcgt gttgcttgcg ggccgtcgca atagcggcgg
      541 caatgctgcc gcaggcggcg atgtggtttt ccttgatcag gaaggcatcg tacaggccga
      601 gacggtggtt gtgacagccg ccggtgcgta ccgcgtattt ctgcgctagg cgcaggcccg
      661 gcagcgtttt gcgcgtatcg agaatcttca cgttcgtgtg catcacgagg tcggcatatt
      721 cgcgcgcggc agtcgcgaca ccggacaagg tttgcagaaa gttcaacgca gtgcgttcgg
      781 cggtcagcag cgcgcgtgcc ggaccactgg ccacgaatat gctgtcttcg ggtcgcacgc
      841 gatcaccgtc acgacgttgc cattgcacgg tgatcgccgg cgacacctgg cggaacactt
      901 catcgaccca ggcttcgccg cagatcacgg catgctcgcg gcacatgacg cgtgcttgcg
      961 ccatctggtt ttccggcacg agcgcggccg tgatgtcgcc actgccgatg tcttcggcga
     1021 gcgcggcggc aactgtttgc gcaagtccgg cgaacaatgg atgcgacgaa aatggatgcg
     1081 acgaagagga aaaggaagaa ctcatcacga tcctgcaatt tgcgaatgtg
//
LOCUS       DASPLT010001444         2195 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_160270, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001444 DASPLT010000000
VERSION     DASPLT010001444.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 2195)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2195)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2195
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_160270"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..272)
                     /locus_tag="VEA63_14745"
     CDS             complement(<1..272)
                     /locus_tag="VEA63_14745"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HYD85321.1"
                     /translation="MNLPNVLTFSRLPAMFAIVALMYAEFQWAATLAFWLFIAAALTD
                     WLDGKIARESGQVSTFGRFMDAVIDKVMVIGVMIALVNGDYFMGHQ"
     gene            503..979
                     /gene="coaD"
                     /locus_tag="VEA63_14750"
     CDS             503..979
                     /gene="coaD"
                     /locus_tag="VEA63_14750"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007359449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HYD85322.1"
                     /translation="MRHCIYPGTFDPITYGHLDVLARAAKLFDRVTVAIAENAGKGPL
                     FSAADRMAMILPNVTPFPNVSVTSFSNLLVEFAVSQNAVAIIRGLRAFSDFEFEFNMA
                     LMNRHLEPRIETIFVMPNEQFSYTSSSLVKQVAKFGGDVTHFVPPNVADALKAAYR"
     gene            1157..>2195
                     /locus_tag="VEA63_14755"
     CDS             1157..>2195
                     /locus_tag="VEA63_14755"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HYD85323.1"
                     /translation="MARPKHSGRIVFTLLLLAAAAAGTWYFLRQRTEQPPEYITAPIN
                     RTDITQAVTATGTLQPVITVDVGSQISGIIDKVLVDFNSEVKAGDVVAHIDSATYESR
                     LRSAEAELANAKANQKLVQLNADRIAQLQQNGLVPQSDLDQVLAQVAQAEAQVQTRTA
                     AVESAKIDLSRCTIYSPVDGIVLSRQVDVGKTVAASFNTPVLFTIANDLTKMQIVGAI
                     AEADIGNVEVGQPVNFTVDAFPNRQFRGRVSSIRNSPQTVQNVVTYDTIIDVRNDDQK
                     LRPGMTANVSVVIAHRPAALRIPNNALRVRLPDVAPPAAAPGAPAPAADGTALKPLPD
                     DQRRTYLTKAGF"
BASE COUNT          422 a          903 c          494 g          376 t
ORIGIN      
        1 atctgatgcc ccatgaagta atcgccattc accagcgcga tcatcacgcc aatcaccatc
       61 accttgtcga tgaccgcatc catgaaccgg ccgaaggtcg acacctgccc gctctcgcgc
      121 gcgatttttc catcgagcca gtcggtcaac gccgccgcga tgaacaacca aaacgccagc
      181 gtcgccgccc actggaactc cgcatacatc agcgccacga tggcgaacat cgccggcagc
      241 cgcgaaaaag tgagcacgtt gggcaagttc atgagttaag caccggagtc cgggaaacca
      301 cacgctgcgc cccgcaaaag caatagcgga cccagcaacg tgggaggagc tttacgcccc
      361 gactcacgcc tccctctcct ccacgcctcc ccccgcccac attctccgct ccatccctcc
      421 gcactccgcg cttcgcccgc cccgccgaaa taaaaaccgc atcgccaccc gcccgccctt
      481 cccctttcgt ccctcgcacg ccatgcgtca ctgcatctac ccgggcacct tcgaccccat
      541 cacttacggt catctcgatg tcctcgcccg cgctgcgaaa ctcttcgacc gcgtcaccgt
      601 cgccatcgct gaaaacgccg gcaagggccc gctcttttcc gccgccgacc gcatggcgat
      661 gatcctgccc aacgtcacgc cgttccccaa cgtctccgtc acgtccttca gcaatctcct
      721 cgtcgagttc gccgtttcgc aaaacgccgt cgccatcatt cgcggcctgc gcgcgttctc
      781 cgatttcgaa ttcgagttca acatggcgct catgaaccgc cacctcgagc cgcgcatcga
      841 aacgatcttc gtcatgccca acgagcagtt cagttacacc agctcatccc tcgtgaaaca
      901 ggtcgccaag tttggcggcg acgtcaccca cttcgtcccg cccaacgtcg ccgacgccct
      961 gaaagccgcc taccgctagc ccgacgtccg cgcagccgat gcgctgaacg ctccagcttt
     1021 ctttcgcctg cgctttgccg cttttgcgcc ttccccgtga cggtgccggc cgcccgtcga
     1081 tcgtcgtcac ctttgtcgcg cccccgcgtc tctacgcgcg attctccaaa cgctgaactc
     1141 accgaacctt tccctcatgg ctcgtcccaa gcactccggc cgcatcgttt tcaccctcct
     1201 gctcctcgcc gccgccgcgg ccggcacttg gtatttcctc cgtcaacgca ccgagcagcc
     1261 gcccgaatac atcaccgcgc caatcaaccg caccgacatc actcaagccg tcaccgccac
     1321 cggcacactc cagcccgtca tcaccgtcga tgtcggcagc cagatttccg gcatcatcga
     1381 caaggtcctc gtcgatttta actccgaggt gaaagccggc gatgtcgtcg cccacatcga
     1441 ttccgccacc tacgaatccc ggctccgctc cgccgaagcc gaactcgcga acgcgaaggc
     1501 caaccaaaaa ctcgtccagc tcaacgccga ccgcatcgcc caactccagc aaaacggcct
     1561 cgtgcctcaa tccgacctcg accaggtcct cgcgcaagtc gcccaagccg aagcccaggt
     1621 ccaaacccgc accgccgccg tcgagagtgc gaaaatcgat ctctcccgct gcaccatcta
     1681 ctcgcccgtc gacggcatcg tcctctcccg ccaggtcgac gtcggcaaaa ccgtcgccgc
     1741 cagcttcaac acgcccgtcc ttttcaccat cgcgaacgat ctcacgaaaa tgcagatcgt
     1801 cggcgccatt gccgaagccg acattggtaa cgtcgaggtc ggccaaccgg tgaacttcac
     1861 cgtcgacgcg tttcccaatc gccagttccg cggacgcgtt tcctccatcc gcaactcccc
     1921 gcagaccgtg cagaacgtcg tcacctacga cacgatcatc gacgttcgca acgacgacca
     1981 aaagctccgc cccggcatga ccgccaacgt ctccgtggtc atcgcccacc ggccggccgc
     2041 gctgcgtatt cccaacaacg cgcttcgcgt gcgactcccc gacgtcgccc cgcccgccgc
     2101 cgctcccggc gctcctgcgc ccgccgcgga tggcaccgcg ctcaaaccgc tccccgacga
     2161 ccaacgccga acctatctca ccaaagccgg cttca
//
LOCUS       DASPLT010001445         1252 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_98129, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001445 DASPLT010000000
VERSION     DASPLT010001445.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1252)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1252)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1252
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_98129"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..768
                     /locus_tag="VEA63_14760"
     CDS             <1..768
                     /locus_tag="VEA63_14760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HYD85324.1"
                     /translation="PGNSGGPLVTGDGRVVGINSRGYAGADNLGFAIPANIAKRVVAE
                     LVRAGEVERSYVGIVPRDLRDFESFYALSLNTGMLINNVDRDSPAARAGIRGGDILLA
                     IDGAQVDGRFPEQLPPIQNLIASRPVGSTLTLTVKRGADTRDYTVVTEKLESRVGEEW
                     AFEKWGVSVRKVSRTYAREHQLSDAGGVLVIGVQPGFPAAVAGVSRGDIITKIGVQPV
                     DTLEVMKQAHAAYEAQPEPVLVEVLRNRQISLYVLKP"
     gene            765..>1252
                     /locus_tag="VEA63_14765"
     CDS             765..>1252
                     /locus_tag="VEA63_14765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal protein PDZ"
                     /protein_id="HYD85325.1"
                     /translation="MTTIFYERLRVAAGLRACLPAKTPRASRQARRPAATLIGLAIFG
                     AGFCGAADLPELWAERVKAVVAIEFYVETEIERRPSFAYGTVVDDRGTIIVPSVAVNA
                     RTTPSQLKDFKVYRAGEATSAAGEYLGQDALTGWHFIRVAESMRSALVPITTFAAAKP
                     VEP"
BASE COUNT          216 a          329 c          476 g          231 t
ORIGIN      
        1 cccggcaact ccggcggacc gctcgtgacg ggtgacggcc gcgtggtcgg gatcaattcg
       61 cgcggttacg cgggcgcgga caatctcggt ttcgcgattc cggcaaacat cgcgaagcgc
      121 gtcgtcgccg agctcgtgcg cgccggtgaa gtcgaacgca gttacgtggg aatcgtgccg
      181 cgcgacctgc gggacttcga aagcttttac gcgctgtcgc tgaacacggg catgctgatc
      241 aacaacgtcg accgcgattc gccggcggcg cgggcgggta ttcgcggcgg cgatattttg
      301 ctggcgatcg acggcgcgca ggtcgacgga cgtttcccgg agcagttgcc gccgatccag
      361 aacctgatcg cgagccggcc ggtgggatcg acgctgacgt tgacggtgaa gcgcggggcg
      421 gacacgcgcg attacacggt ggtgacggag aaactcgaga gccgggtggg cgaggagtgg
      481 gcgtttgaaa aatggggcgt gagcgtgcgc aaggtgtcgc gcacgtatgc gcgcgagcat
      541 cagttgagcg atgcgggcgg cgtgctggtg atcggcgtgc agccgggatt tccggcggcg
      601 gtcgctggcg tgtcgcgcgg cgacatcatc acgaagatcg gtgtccaacc tgtggatacg
      661 ctagaagtga tgaagcaggc gcacgcggcg tatgaagcgc aaccggagcc ggtgttggtg
      721 gaagtgctgc gcaaccggca gatttcgctg tatgttttga aaccatgacg acgattttct
      781 acgaacgttt gcgtgtagcg gcaggcctcc gtgcctgcct gccggcgaaa acacctcgag
      841 ccagcaggca ggcacggagg cctgccgcca cgcttatcgg gctggcaatt ttcggcgcgg
      901 gattttgcgg ggcggcggat ttgccggagc tttgggcgga gcgggtgaag gcggtcgtcg
      961 cgatcgagtt ttatgttgaa acggaaatcg agcggcggcc gagttttgct tatggcacgg
     1021 tcgtcgatga tcgagggacg atcatcgtgc cgtcggtcgc ggtgaatgcg cgcacgacgc
     1081 cgtctcagtt gaaggatttc aaagtgtatc gcgcgggaga agcgacgagt gcggcgggtg
     1141 agtatctcgg gcaggatgcg ttgacggggt ggcatttcat tcgcgtggcg gagtcgatgc
     1201 gcagcgcgct ggtgccgatc acgacgttcg cggcggcgaa gccggtggag cc
//
LOCUS       DASPLT010001446         1772 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_160278, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001446 DASPLT010000000
VERSION     DASPLT010001446.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1772)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1772)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1772
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_160278"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..453
                     /locus_tag="VEA63_14770"
     CDS             <1..453
                     /locus_tag="VEA63_14770"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HYD85326.1"
                     /translation="DALGPLVEFFTDGSRADYSVMQGTLPPRGVVPLHSHPDDESFFL
                     IGGTLQALVEHRGSFEWFDVPLLGFVHVPKNAKHAWRNVGDEPVVQLIVTSTRLGQFF
                     RDACRPLGEDEPTRPPTPEDLARFTQAAKTYGHWLASPQENAAVGILM"
     gene            complement(583..1650)
                     /locus_tag="VEA63_14775"
     CDS             complement(583..1650)
                     /locus_tag="VEA63_14775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family protein"
                     /protein_id="HYD85327.1"
                     /translation="MLTRRSFVTHLAALPLALAAARAAEITKTAPSLSAFARRLRPVG
                     RILELDGYYVWCQAPIFGPDGRVHVFFSRWPAARNMGGWISSSEIAHAVAASPEGPYE
                     VTGTVLAPRGPGHWDATTCHNPHVQKVGDRYALFYMGNRNGRTDTKRIGLALADNLDG
                     PWERPDAPLLEPGPLGAWDNHCTTNPSLLQQPDGKFWLYYKSWNTADYEAGKPPVRGN
                     RKYGLAIADRLEGPYRKHEKNPLIDFSHRGENAQCEDAFIWRQNDRVHAVLRDMGVFS
                     HDVGLFLDSPDGLAWSEPTIAYEPLRTYASEPPPPPKLNRYGRLERPQLLLRDGVPAY
                     LYTATQGGRYGTASGFLFALS"
BASE COUNT          313 a          530 c          608 g          321 t
ORIGIN      
        1 gatgcgctcg gaccactggt ggaatttttc acggacggat cgagagcgga ctacagcgtg
       61 atgcaaggca cgctgccgcc tcgcggtgtc gtgccgcttc acagtcatcc ggatgacgaa
      121 agtttctttc tgatcggcgg aaccctgcag gcgctggtgg aacatcgcgg cagcttcgag
      181 tggttcgatg ttccgcttct gggattcgtc catgtgccga agaacgcaaa gcatgcgtgg
      241 cgcaatgtcg gcgatgagcc ggtggtgcag ttgatcgtga cgagcacgcg actcgggcaa
      301 ttttttcgcg acgcatgccg cccgctcggc gaagacgagc cgacgcgtcc gcccacgccg
      361 gaagatttgg cgcgcttcac gcaagccgcg aagacctatg gccactggct ggccagtccg
      421 caggagaacg cagcggtggg catcttgatg tgacgcgaag cgcgcgagaa aatcgccagc
      481 cgaactcatg cagcaaggtg gagcccaacg cccccgtcgg gctccgcctt cggtgcgaag
      541 ctccgtcgtc ggtcgggcgg gcgtccggtg tgtgagtccg gctcagctca aggcgaaaag
      601 aaatcccgac gccgttccgt aacgaccgcc ctgcgtggcg gtgtagagat acgcgggcac
      661 gccgtcgcgc agcagcaatt gcggacgctc gaggcggccg tagcgattga gcttcggtgg
      721 cggcggcggc tcgctcgcgt aggtgcgcaa gggctcgtag gcgatcgtgg gctcggacca
      781 cgcgaggcca tccggcgaat cgaggaacag cccgacgtca tgactgaata cgcccatgtc
      841 gcgcagcacg gcgtggacgc gatcgttttg cctccagata aacgcatcct cgcactgcgc
      901 attttcgccg cggtgggaga aatcgatcag cggatttttt tcgtgtttcc gataaggccc
      961 ttcgaggcgg tcggcgatgg cgagaccgta tttgcggttg ccgcgcacgg gcggttttcc
     1021 ggcttcgtag tccgccgtgt tccacgactt gtagtagagc cagaacttgc catcgggctg
     1081 ctgcagcagc gacgggtttg tcgtgcaatg gttgtcccac gcgccaaggg gaccgggttc
     1141 aagcagcgga gcatcgggcc gctcccaagg accgtcgagg ttgtccgcga gcgcgagccc
     1201 gatgcgcttc gtatccgtgc ggccgttacg attgcccatg tagaacaacg cgtagcgatc
     1261 gccgactttc tgcacgtgcg gattgtggca cgtcgtggcg tcccagtgac ccgggccgcg
     1321 cggggcgagg acggtgcccg tgacttcata aggtccttcc ggcgaagccg ccaccgcgtg
     1381 ggcgatttcc gaagagctga tccaaccgcc catgtttcgg gcggccggcc agcgggagaa
     1441 aaacacgtgc acgcgtccgt ccggaccaaa gatcggcgcc tggcaccata cgtagtagcc
     1501 gtctaattca agaattcggc cgacgggccg gagccgccgg gcgaaggccg aaagcgacgg
     1561 agcggttttc gtgatttcgg cggcccgagc tgccgctaac gcgagcggaa gcgcggcgag
     1621 atgggtgaca aatgaacgac gtgtgagcat gagcgcgagt gcagcgccgt cgtctggggc
     1681 atctacttcg tcgccggctc cggttggtag tagcgcgtca tcgcgtccgc gatcgctgcg
     1741 accgttgcgg cgcgatcgat cacgccgttg tg
//
LOCUS       DASPLT010001447         1704 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_17696, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001447 DASPLT010000000
VERSION     DASPLT010001447.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1704)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1704)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1704
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_17696"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1269
                     /locus_tag="VEA63_14780"
     CDS             <1..1269
                     /locus_tag="VEA63_14780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HYD85328.1"
                     /translation="TLLAAVRKVYVSVLDSEALKYRRRRNLLESEERMALLIMRVSGA
                     PHGRYYYPQAAGVGLSYNPFVWHPDIDPRAGVLRLVFGLGTRAVDRADDDYTRLVALN
                     APTRRPEASFEEVRDHSQRRMDCLDLSTGRFLSAPFGDVLADAVDYPTDLYLTPTDED
                     GYPCITFERLLRETKVADDLRRMLEVLEKAYEAPVDIEFALNFIADGTYRIHLLQCRT
                     FQVRREGSALSAVSGKETRLLAANGAVIGVSREQRLNRIVYVVQETYAKLNEQERYAV
                     ARLIGRLNPTYPVGDRGLMLIGPGRWGTQSPALGIPVSFAEISRASVICEVVAMHERL
                     IPDVSLGTHFFNDLVEHDTLYVAYFPRKSGNTLDTEWFKTAPNQLLELEPSATALENI
                     VRVIDCNAIGGGVFLRADATAQEAVVLRVE"
     gene            1419..>1704
                     /locus_tag="VEA63_14785"
     CDS             1419..>1704
                     /locus_tag="VEA63_14785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD85329.1"
                     /translation="MSLQPQFSVSARLLRELEAVAELRTKIAEATIQVAWIPQLQKDS
                     RQRGAHASTAIEGNPLTLEEVRALESGAPLPARPERSQREVLNYFAGLRWV"
BASE COUNT          313 a          449 c          615 g          327 t
ORIGIN      
        1 acgctgcttg cggcggtgcg gaaggtctat gtgagcgtgc tggacagcga ggcgctcaag
       61 tatcggcggc ggcgaaacct gctcgagagc gaggaacgga tggcgttgct gatcatgcgc
      121 gtgtcgggcg cgccgcatgg caggtattac tatccgcagg cggcgggcgt gggactttcg
      181 tataacccat tcgtttggca cccggacatc gatccgcgcg cgggcgtgct gcgactggtg
      241 tttgggttgg ggacgcgagc ggtcgatcgc gcggatgacg attacacgcg gctggtggcg
      301 ttgaacgcgc cgacacggcg accggaggcg agcttcgagg aagtgcggga tcattcgcag
      361 cggcggatgg actgcctcga tttgtcgacc gggcggtttt tgtcggcgcc gtttggcgac
      421 gtgctggcgg acgcggtgga ttatcctacg gacctctacc tgactccgac ggatgaggac
      481 ggctatccgt gcatcacctt cgagcgacta ctgcgcgaga cgaaggtggc ggacgatctg
      541 cggcgcatgc tggaggtgct cgagaaagcg tatgaggcgc cggtcgacat cgagtttgcg
      601 ctgaacttca tcgcggacgg gacgtatcgg attcacctgc tgcaatgccg gacgtttcag
      661 gtgcggcgag aaggcagcgc gttgtctgct gtatccggaa aagaaacacg cctgctggcg
      721 gcgaacgggg cggtgatcgg cgtcagccgg gaacagcggc tgaatcgaat cgtgtatgtc
      781 gtgcaggaaa cctatgcgaa gctgaacgag caagagcgct acgccgtcgc acggctgatc
      841 ggacgattga acccgactta cccggttggc gatcgcgggc tgatgctgat tggacccggg
      901 cgttggggaa cgcagagccc ggcgctcggc attccggtga gcttcgcgga gatcagtcgc
      961 gcgtcggtca tctgcgaagt ggtcgcgatg cacgagcggt taattccaga tgtgtcgctg
     1021 gggacgcatt tcttcaacga tctcgtggaa cacgacacgt tgtatgtggc gtattttccg
     1081 cggaagtcag gcaacacgct ggacacggaa tggttcaaga ccgcgccgaa tcagctgctg
     1141 gaactggaac cgtcggcgac ggcattggaa aacatcgtgc gggtgatcga ttgcaatgcg
     1201 attggcggcg gggtgtttct gcgtgcggac gcgaccgcgc aagaggcagt ggtgttgcgg
     1261 gtggagtgag gcagtgacgg agaaggcaac cggctgaaac cagcccgccg gagcgtctcg
     1321 cgaagtcgcg cgacgaactt gtcagatttc tggactcatg cttgcccaat ggcagcgatt
     1381 aacttgactg atcacttgac ttacgcttgt cttggagcgt gagccttcag ccccagtttt
     1441 ctgtttcggc gcggctgctc cgcgagctgg aagccgtggc ggagctgcgg acgaagatag
     1501 cggaagcgac gattcaggtc gcgtggatcc cgcagttgca gaaggacagc cgacaacgcg
     1561 gcgcgcatgc gtcgacggcg atcgaaggca atccgctcac actcgaggag gtgcgggcgc
     1621 tcgagtcagg tgcgccgctt ccggcgcgac ctgaacgctc gcagcgcgag gtgttgaact
     1681 atttcgcggg cctgcgttgg gtgg
//
LOCUS       DASPLT010001448         1599 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_221761, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001448 DASPLT010000000
VERSION     DASPLT010001448.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1599)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1599)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1599
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_221761"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1100)
                     /locus_tag="VEA63_14790"
     CDS             complement(<1..1100)
                     /locus_tag="VEA63_14790"
                     /inference="COORDINATES: protein motif:HMM:NF024883.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HYD85330.1"
                     /translation="MKSGQEGGETVAGFEKTRFSSNAALAAWVQEIAPFGIFTTDGEL
                     RIRSWNQWLVAHSGLPADAVVGRPLIEVFPNLAERRLHGHFQRALAGEISVLSTALHQ
                     YLLPLRVTVPDSALPHMLQTARIAPLPDEGAIGGTITIIEDVTQREVQAAILHRQQEL
                     DRLLSSALAALLQSTDPAKDVAEIFPTIAPSLGLDAYLSYLLDADGRTLRLNASGGVS
                     PKQREALGTFEVSERDRMGAGRLRPGIPGTVATHEEELQLIGLRARCTFPLTIGERVL
                     GLVSFGSYERASIATADVNTLARIARYVAIALDRTLRERDAVAASRAKDDFLAALSHE
                     LRTPLNPVLLLASDSAANQEYSASAREAFRVI"
     gene            complement(1097..>1599)
                     /locus_tag="VEA63_14795"
     CDS             complement(1097..>1599)
                     /locus_tag="VEA63_14795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="chemotaxis protein CheC"
                     /protein_id="HYD85331.1"
                     /translation="VTMHEIDAIAPMLEKVINVDVASVNQVFHGPISGNALLMLDERA
                     AMVLSRLLTDDRTGSTDFDSSAREIITEVGNILLNACLGVFGNLLHVHVTFSVPQLHV
                     ESVTRVLRAVAEQGTEQMRYGLMIHTRFFVRAGDVTGYLVIILGITSLDRLLAELENW
                     ENRQAQ"
BASE COUNT          289 a          589 c          445 g          276 t
ORIGIN      
        1 tcgatcacgc gaaacgcctc gcgcgcgctc gccgaatact cctggttcgc cgccgaatcg
       61 ctcgccagca acaacaccgg attcagcggc gtccgcagct cgtgcgacaa cgccgccaga
      121 aaatcgtcct tcgcgcgcga cgccgccacc gcgtcccgtt cgcgcagcgt ccggtcgagt
      181 gcgatcgcga cgtaacgcgc gatccgcgcc agcgtgttca catccgccgt cgcgatgctc
      241 gcgcgctcat agctgccgaa cgacacgagc ccgagcacgc gttcgccgat cgtcagcgga
      301 aacgtgcaac gcgcccgcag cccgatcaat tgcagctcct cctcgtgcgt cgccaccgtg
      361 ccgggaattc ccggtcgcag ccggcccgct cccatccggt cgcgttcact cacctcgaac
      421 gttcccagcg cctcgcgttg tttcggcgaa actccgcccg acgcgttcaa ccgcaacgtg
      481 cggccgtcgg cgtccaacag ataactcaga tacgcgtcga gccccagcga cggcgcgatc
      541 gtcggaaaaa tttccgccac gtccttcgcc ggatccgtcg attgcagcag cgccgccagc
      601 gccgacgaca gcagccggtc gagctcctgc tgccggtgaa gaatggccgc ctgcacttca
      661 cgctgcgtca cgtcctcgat gatcgtgatc gtcccgccga tcgcgccttc atccggcagc
      721 ggcgcgatcc gtgcggtttg cagcatgtgc ggcagcgcgc tgtccggcac cgtcacacgc
      781 aacggcagca ggtattggtg caacgccgtc gagagcacgc tgatctcgcc cgcgagcgcg
      841 cgttgaaaat gtccgtgcaa tctccgctcc gccaaattcg gaaacacctc gatcagcggc
      901 cgccccacca ccgcgtccgc cggcagcccg ctgtgggcca cgagccactg gttccagctc
      961 cggatgcgca gttcgccgtc ggtcgtgaaa atcccaaagg gcgcgatctc ctgcacccac
     1021 gccgccagcg ccgcattcga gctgaagcgg gttttctcga aacccgccac cgtctcgcca
     1081 ccttcttggc cgcttttcat tgcgcctgcc ggttttccca gttctccaat tccgccagca
     1141 gacgatcgag tgacgtgatg ccgaggatga tcacgagata accggtcaca tcgccggccc
     1201 tcacgaaaaa ccgcgtatga atcatcagcc cgtaacgcat ctgctcggtg ccttgttccg
     1261 ccaccgcgcg cagcacgcgc gtgacacttt caacatggag ctggggaacg gagaaagtga
     1321 catggacgtg cagcaggttg ccaaacaccc ctagacacgc gttcagcaga atgttgccga
     1381 cctccgtgat gatttcccgc gcgctcgaat cgaaatccgt cgagcccgtg cgatcgtcgg
     1441 ttaacagccg gctcaacacc atcgccgcgc gctcatcgag catcagcagc gcgttgcccg
     1501 agatcggccc gtggaaaacc tggttcacgc tcgcgacgtc gacgttgatc accttctcca
     1561 gcatcggcgc gatcgcgtcg atctcgtgca tcgtcacct
//
LOCUS       DASPLT010001449         1210 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_160334, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001449 DASPLT010000000
VERSION     DASPLT010001449.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1210)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1210)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1210
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_160334"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..366
                     /locus_tag="VEA63_14800"
     CDS             <1..366
                     /locus_tag="VEA63_14800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD85332.1"
                     /translation="LPDSGSLVVGSKATVLADTYCQSVRIVPEARMRELAPTLPAKTL
                     PRCREGHYYEWVNACKGGPAAASNFDYAGPFSETVLLGNVAIRARKRIEWDAANLRIA
                     NVPDANRFLTKEYRPGFGV"
     gene            474..>1210
                     /locus_tag="VEA63_14805"
     CDS             474..>1210
                     /locus_tag="VEA63_14805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD85333.1"
                     /translation="MPFNFNFTNPRARVFRLATVGLSLAGFAFGGVVAQTAVPAVDAG
                     SMTKMPLDPLGNLVLLEEYIVSDETGSPNYDPTGLGADEAERIEAPFGSDLLADVAAE
                     DLPMGELDVELGYASGANPVEIALGGDRLNLRGFPTPLRRNGFTQSGFPEVINSERVE
                     TIMGALTPVTGRAAPGGIRNFLTARPRGRTARQVSVSAGTNERWSAEAKATGVLKAKK
                     AWHALTAGARGSHGPQPFAEDTVAYFSG"
BASE COUNT          199 a          366 c          431 g          214 t
ORIGIN      
        1 ctcccggaca gcggctcgct ggtggtcggc agcaaggcga cggtgctggc ggacacctat
       61 tgccagagcg tgcgcatcgt gcccgaggcg cgcatgcgcg aactcgcgcc gaccctgccg
      121 gcgaagacgc tgccgcgttg ccgggagggg cattactacg aatgggtgaa cgcctgcaaa
      181 ggcgggccgg cggcggcttc gaacttcgac tacgccgggc ctttcagtga aacggtgctg
      241 ctgggcaacg tggcgatccg cgcgcgaaaa cgcatcgagt gggacgccgc gaatcttcgc
      301 atcgccaacg tgcctgatgc gaatcgcttt ctcacgaaag agtatcgacc gggttttggc
      361 gtgtgaggcg tcgaatcgaa tcgccgcggt ccgttcgcgc ggcgcgagga ttgccaaggc
      421 ccgagttggc cggcgtattc acggttccta actccgcaac gaccgctgcc gacatgcctt
      481 tcaatttcaa cttcacgaat ccccgcgcac gagtttttcg actggcgacc gttggtctgt
      541 cgctcgctgg atttgccttc ggtggcgttg tcgcgcagac cgcagtcccc gcggtcgacg
      601 ccggctcgat gacgaagatg ccgctcgatc cgttgggcaa tctcgtgctg ctggaggaat
      661 acattgtgag cgacgagacc ggttcgccga attacgatcc gacgggtttg ggtgcggacg
      721 aagcggagcg catcgaggcg ccatttggtt cggacctgct cgcggatgtc gcggcggagg
      781 atctgccgat gggcgagctc gacgtcgaac tgggctacgc gtcgggtgcc aatcccgtgg
      841 agatcgcgct cggtggcgac cggcttaatt tgcgcgggtt tccgacgccg ctgcggcgaa
      901 atggattcac gcagtcgggc ttccccgagg tgatcaacag cgagcgcgtt gagaccatca
      961 tgggcgcgct cacgccggtc accggtcgag cggcgccggg cggtatccga aatttcctga
     1021 cagcgcggcc gcgcggccgg acggcgcggc aggtgtccgt gtccgccggg acgaacgagc
     1081 gctggagcgc cgaggcgaag gccaccggcg tgttgaaagc gaagaaggcg tggcatgcgt
     1141 tgaccgcggg ggcgcggggc agccacggtc cgcagccgtt cgcggaagac accgtggcgt
     1201 atttttccgg
//
LOCUS       DASPLT010001450         1834 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_17736, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001450 DASPLT010000000
VERSION     DASPLT010001450.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1834)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1834)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1834
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_17736"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            326..793
                     /locus_tag="VEA63_14810"
     CDS             326..793
                     /locus_tag="VEA63_14810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD85334.1"
                     /translation="MKTSCHSTLALTFFVAIAATNLPANPTAQRGARTEQPGRGELGA
                     QLDRNLAAGQRTLALARHQAKELRGEARERFEAAAADVQAAERRLRKNLEDVKDASVD
                     EWQDARLTLATAYEAYARAIAEVQRIASATTKSEDRGIYFSRQRRASAERDRP"
     gene            881..1522
                     /locus_tag="VEA63_14815"
     CDS             881..1522
                     /locus_tag="VEA63_14815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite oxidase-like oxidoreductase"
                     /protein_id="HYD85335.1"
                     /translation="MSKARYIEAKQRWAEKQIARGVTPRAVASTERLPPGQKLTSGFP
                     VLDLGVQPEIPLSEWKLTLGGLVERPTTLTWEQLKALPQVEDVSDFHCVTTWSKYDCR
                     WGGVAFTTLYELVQPKPAAQFVYFTSYDGYSTNVSLARCLDDDVLLATEFDGAPISRE
                     HGGPARVIIPKLYAWKGAKFVNGITFLEEDKLGFWEVRGYSNSADPWKEERYA"
     gene            1549..>1834
                     /locus_tag="VEA63_14820"
     CDS             1549..>1834
                     /locus_tag="VEA63_14820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD85336.1"
                     /translation="MLEHCPPKRCLHFAQAVPRFDPSHGVSKAARFDRQQKRSSRTFS
                     TGNAHAGFDLEEELKGVDTEHTAKRESRFTAGDENALNIVLDDEPGRLRDN"
BASE COUNT          398 a          534 c          590 g          312 t
ORIGIN      
        1 ggaaacctcg agcacgggcg tcatgggtga gcgaccagca aactgaagtg aacgagtaac
       61 gagccgatga gcgcggacat ctgcgcgatg tcacagcttt gcgcccgtgc cgcaagaaag
      121 gagtgggttg cagaattccg gctgacagcc cgcgcggtca tgcacgatgc gggaacgatg
      181 cggcgacaaa agggcgcaca tttcttctag tcccggtttg gcagcaagtt gaccggctgg
      241 cgccgctgac ggcacgccga atgcaaaaat gaagcaacca atcaccgact tcccgcatga
      301 gcgccaaacg ttcaactccc ccgtgatgaa aacatcgtgt cactcaacgc tcgcgctgac
      361 ctttttcgtt gcgatcgcag cgacaaattt gccggcgaat ccgacggcgc aaagaggggc
      421 tcgcacggag cagcccggcc gcggcgaact cggcgcccag ctcgatcgca atctcgcggc
      481 ggggcaacgc accttggcgc tggcacggca ccaggcgaag gagttgcgcg gagaagcgcg
      541 tgagcggttc gaagcagccg ccgccgacgt gcaggccgcc gagcggcgcc tgcgcaagaa
      601 tctggaagac gtgaaagacg cgtcggtcga cgaatggcag gacgcgcgcc tgacgctcgc
      661 aacggcgtat gaagcttacg cgcgggcgat cgcagaagtt caacgtatcg cgagcgccac
      721 tacaaaatcc gaggatcgcg gaatatattt cagccggcag cggcgcgcgt ccgcggagcg
      781 cgatcgaccc tgaagtcatc gacgccgctc cgtgccgcgc aacagctgcc gcgcggcacg
      841 gcgcttgcga agtgcgtgac gagaggcacg ctatgtcggc atgtcgaaag cacgctacat
      901 cgaggcgaaa cagcgttggg ccgagaagca gatcgcgcgc ggtgtcacgc ctcgcgcagt
      961 cgcttcgacc gagcggttgc cgccggggca aaagttaacc tcagggtttc ctgttttgga
     1021 tctcggcgtg cagccggaga ttccgctgag cgaatggaag ctgaccttgg gcggactggt
     1081 cgagcgtcct acgactttga cgtgggagca gctcaaggcc ttgccgcagg ttgaagacgt
     1141 gagcgatttc cactgtgtga cgacgtggag caaatacgac tgtcggtggg gtggcgtggc
     1201 gttcaccacg ctttacgagc tcgtccaacc gaagcccgcg gcgcagttcg tctatttcac
     1261 gagttacgac ggctactcga cgaacgtttc attggcgcgc tgcttggacg acgacgttct
     1321 actggccacg gaattcgatg gtgcgccgat ttcgcgcgag cacggcgggc cggcgcgcgt
     1381 gatcatcccg aagctttatg cgtggaaagg cgccaagttc gtcaacggca tcacatttct
     1441 agaggaggac aaactcgggt tctgggaagt tcgcggttat tcgaacagcg ccgatccctg
     1501 gaaggaagag cgctacgcgt aagtcggagc gagaagtccg gaggagcaat gctggaacat
     1561 tgcccgccaa agcggtgctt acatttcgcg caggcggtcc cacgctttga tccaagtcac
     1621 ggcgtctcca aagccgccag gtttgatcgc cagcaaaaga ggtcctcgcg aactttctcc
     1681 acaggaaacg ctcacgccgg gttcgatctc gaagaggaac tcaagggtgt cgacaccgag
     1741 cacacggcaa agcgcgaaag ccgtttcacc gccggtgatg aaaacgcgtt gaatattgtt
     1801 ttggatgatg agcctggccg cctccgcgac aatc
//
LOCUS       DASPLT010001451         1799 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_181907, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001451 DASPLT010000000
VERSION     DASPLT010001451.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1799)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1799)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1799
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_181907"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(129..632)
                     /locus_tag="VEA63_14825"
     CDS             complement(129..632)
                     /locus_tag="VEA63_14825"
                     /inference="COORDINATES: protein motif:HMM:NF024822.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4112 domain-containing protein"
                     /protein_id="HYD85337.1"
                     /translation="MGAVTRIPKLPSTDADRRFQRLRRLVWWLDRSFGFGPRGRFGLD
                     PLLGLIPGIGDWAGAALSLYIVYEAMRLGLPWPVLGRMVANIAIESLIGTLPVAGDAF
                     DFYWQANARNLRLVEQHYSPKLKARPFRSVPLFIGALALFLVAMLAATLTLAAWGVRE
                     LVQFVVS"
     gene            complement(863..1402)
                     /locus_tag="VEA63_14830"
     CDS             complement(863..1402)
                     /locus_tag="VEA63_14830"
                     /inference="COORDINATES: protein motif:HMM:NF027567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HYD85338.1"
                     /translation="MNTPNTTQGRAEALNKLRDLINDINFAMLTTVAADGSLRSRPMA
                     TLQFDTETGELWFFTAQDSPKADEIAEEHQVGVSYASPGKQDYVSISGRARIVRDQSK
                     MAELWTPMAKLWFPKGLDDPRLALLRVEIEAAEYWDSPSGMMVGLYGLAKLAITGKPP
                     KHTGENVKIDLPLKERARY"
     gene            1550..>1799
                     /locus_tag="VEA63_14835"
     CDS             1550..>1799
                     /locus_tag="VEA63_14835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HYD85339.1"
                     /translation="MVPFSILDLSPIVQGGTAAQAFQRSRELAQHAERLGYRRFWLAE
                     HHNMPGIASAATAVVIAHVAAGTKTIRVGSGGVMLPNHA"
BASE COUNT          357 a          493 c          612 g          337 t
ORIGIN      
        1 gctggcgggg cgttgtttcc aatacgctgc aagaatttcc aatggaatgg aacgtgaaac
       61 gcggaccaac ctaccggatt atgaccgagg taaaatcccc tatggcacga tcaatgtcgc
      121 gcttacgctt agctgacgac gaactgaacc agttcgcgga caccccatgc cgcaagcgtc
      181 aacgtggcgg cgagcatcgc gacgagaaac aaagcgagcg caccgataaa cagcggcacg
      241 ctgcggaacg gacgagcttt gagctttggg ctgtagtgtt gttcgacgag acgcaggttg
      301 cgggcgttgg cctgccagta gaaatcgaac gcgtcgcccg cgaccggcaa cgttccgatg
      361 agggattcga ttgcgatgtt ggcgaccatg cgcccgagca ccggccaagg gaggcccagc
      421 cgcatggctt cgtagacaat gtagagcgat agcgctgcac ccgcccagtc tccgattccg
      481 gggatcaaac cgagaagcgg atcgagcccg aagcggcctc gagggccaaa accaaacgat
      541 cggtcgagcc accacacgag ccggcgaagc cgttgaaagc gccggtcggc atcggtcgag
      601 gggagctttg ggatgcgggt gacggcaccc atggttggag cgaatggcta tccggatcgg
      661 ataatcacag ccaaataaat cgagcttgtt acacggagat cacagagttc tgacacagag
      721 ggacccagga ggccacgggg tatttgatgg tctttccggc agacgaaggc tgatggcggt
      781 gaagacttgg gtccgcatca agccggctgc gtcgctatcc cgcgcggcgc gcgcaagacg
      841 cgggatgcga tgcgacgatc aatcaataac gcgcgcgctc ttttagcggc aggtcgatct
      901 tcacgttttc cccggtgtgc ttcggtggtt tgccggtgat cgcgagtttc gcgaggccgt
      961 ataagccgac catcatgccg gacggagagt cccaatattc cgcggcttcg atctcgacgc
     1021 gaagtaaggc gagccgcgga tcgtcgagcc cctttggaaa ccagagtttc gccataggcg
     1081 tccacagttc ggccatttta gattggtcgc ggacgatgcg ggcgcggccc gagatcgaca
     1141 cataatcctg tttgcccggg gaggcatagg agacgccgac ttgatgttct tcggcgatct
     1201 cgtcggcctt gggcgagtct tgcgcggtga aaaaccagag ctcgccggtc tcggtgtcga
     1261 attgcagcgt cgccatcggc cggctgcgca acgagccgtc ggcggcgacg gtggtgagca
     1321 tggcgaagtt gatgtcgttg atgagatcgc gaagtttgtt cagcgcttcg gcgcggcctt
     1381 gagttgtgtt cggagtgttc atcgttgggc ggtagaccgg agacggtaac caatgatcac
     1441 ggacaagcgt cgggggggac tgcgcgcggg cgtttttggg gtaaaacccg ggtggtttcg
     1501 cgcgcgagcg gcggctggca aaaccgcgac gggtgcgctc attgcgcgca tggttccgtt
     1561 ttcaattctg gatctttcgc cgatcgtgca aggcggcacg gcggcgcagg cgtttcaacg
     1621 ttcacgcgag ctcgcgcagc atgcggagcg gctgggttac cggcgtttct ggctcgcgga
     1681 gcaccacaac atgccgggca tcgcgagcgc ggcgacggcg gtggtgatcg cgcacgtcgc
     1741 tgcggggacg aagacgattc gcgtcggttc gggcggcgtg atgttgccga atcatgcgc
//
LOCUS       DASPLT010001452         1094 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_241620, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001452 DASPLT010000000
VERSION     DASPLT010001452.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1094)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1094)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1094
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_241620"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            232..651
                     /locus_tag="VEA63_14840"
     CDS             232..651
                     /locus_tag="VEA63_14840"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070497 -
                     6-carboxy-5,6,7,8-tetrahydropterin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HYD85340.1"
                     /translation="MPFRIAKTFTVESGHLLTKHPGACRFPHGHSRTIEIVITANELD
                     ANDMVCDFKALKAAARDAVERFDHTFALNTADPHFAQLRALYGDRVIAFNHTDPTTEV
                     LARDIFHAVRRQLALPRARLERVRVTETATSWGEYWE"
     gene            766..>1094
                     /locus_tag="VEA63_14845"
     CDS             766..>1094
                     /locus_tag="VEA63_14845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HYD85341.1"
                     /translation="MSAELSELYQQVILDHNKRPRNRGILPTANRVAHGDNPSCGDQC
                     SVYLRLDGDRIAEITFDGSGCAISQASASLMTTQLKGKTAADAAKLYDDFKHIVTTGQ
                     APEEISD"
BASE COUNT          217 a          445 c          268 g          164 t
ORIGIN      
        1 aggcccggca tctgtcgaag gggcgaaagg aaggccgccc cattccccgg gtcaacgcgt
       61 aattgcgccc gcaatcgctc ctccacggga cgcgccaacg gctttgccca caacctgcgg
      121 acaacctggt gcaagaatcg ccgactgttt cgcaagctgc gcgcggccgc ttcgctcacg
      181 cgcaggcaat atccccctcg cctcccgccc gtcgcgcgct cgcaccacac catgccattc
      241 cgcatcgcca aaaccttcac cgtcgaaagc ggtcacctgc tcaccaagca cccgggtgcc
      301 tgccgttttc cccacggcca cagccgcacg atcgaaatcg tcatcaccgc caatgaactc
      361 gatgccaatg acatggtgtg cgatttcaag gcgctcaaag ccgccgcccg tgacgccgtc
      421 gaacgcttcg atcacacctt cgccctcaac accgccgacc cgcacttcgc tcaactccgc
      481 gccctctacg gcgatcgcgt catcgccttc aaccacaccg atcccaccac cgaagtgctc
      541 gcgcgggaca ttttccatgc cgttcgccgc cagctcgctc taccccgcgc gcggctggag
      601 cgcgtgcgcg tcaccgaaac cgcgacaagc tggggcgaat actgggaata accgcttcgc
      661 ggcgatcccg cattccgcgc cggacgccag ccgcccgacg ccggccgcgc tgacgagttg
      721 ccatccgcac cacgccgctc gacagtcgac gtccgccttt tcgccatgtc cgccgaactc
      781 tccgaactct accaacaggt catcctcgac cacaacaaac gcccccggaa ccgcggcatc
      841 ctccccacgg ccaatcgcgt cgcgcacggc gacaaccctt cctgtggcga ccagtgcagt
      901 gtctacctgc gactcgatgg cgatcgcatc gccgaaatca ccttcgacgg ctccggctgc
      961 gccatctccc aagccagcgc ctcgctcatg accacccagc tcaaaggcaa aaccgccgcg
     1021 gacgccgcga aactctacga cgacttcaaa cacatcgtca ccaccggcca ggcacccgag
     1081 gagatcagcg acat
//
LOCUS       DASPLT010001453         1112 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_202190, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001453 DASPLT010000000
VERSION     DASPLT010001453.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1112)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1112)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1112
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_202190"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1112
                     /locus_tag="VEA63_14850"
     CDS             <1..>1112
                     /locus_tag="VEA63_14850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012377128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter TolR"
                     /protein_id="HYD85342.1"
                     /translation="RKLGWIARSSLDADSPYADACLHGDGLTSLQYRATKGAETQQIE
                     LPVKGGDVVQLERRGNTYIFSSARYGEPFVSATMDLDLGEELFVGLFLCSHNPAVKEE
                     AIFKDVRIVRPPKAGYVPYRDYIGAHLEVLNVFTGQLAVVHSSPEQFEAPNWMHDGKT
                     LLINVSGPGPNKGHLKTFDLTTKTIGDFDTGFATRNNNDHVLTFDGKQLAISHHSADD
                     NGRSVVYKLPATGGTPVRVTPNAPSYFHGWSPDAKWLVYTGGRKHTPDAKSDKYDIYK
                     ISADGGEEIRLTDAPGLSDGPEFSPDGKYIYFNSTRSGLMQIWRMKPDGSGQEQLTND
                     EFNNWFPHISPDGKWIVFISYGQDIKPEEHPYYKHV"
BASE COUNT          245 a          335 c          338 g          194 t
ORIGIN      
        1 cgcaagctcg gctggatcgc gcggtcttcg ctcgacgcgg actcgccgta tgccgacgcg
       61 tgcctgcacg gcgacggact cacgtcgttg cagtatcgtg cgacgaaggg agcggagacg
      121 cagcagatcg agctgccggt caagggcggc gacgtcgtgc aactcgagcg gcggggcaac
      181 acctacatct tttcgtcggc gcgttacggc gagccgttcg tgtcggcgac gatggacctc
      241 gatctcggcg aagagctttt cgtggggttg ttcctttgct cgcacaaccc ggccgtgaag
      301 gaagaagcga tcttcaagga cgtgcgcatc gtgcgtccgc cgaaggccgg ctacgtgccg
      361 tatcgcgact acattggcgc gcacctggag gtgctcaacg tgttcaccgg ccaactcgcg
      421 gtcgtgcaca gttcgcccga acagttcgaa gcgccgaact ggatgcacga tggcaaaacg
      481 cttctgatca acgtgagcgg accggggccg aacaaagggc atctgaaaac cttcgatctc
      541 acgacgaaga cgatcggcga cttcgacacg ggtttcgcga cgcgcaacaa caacgaccac
      601 gtgctgacgt tcgacggcaa gcagctcgcg atcagccatc acagcgcgga cgataacggc
      661 cgttcggtcg tctacaaact cccggcgacc ggcggcactc cggtgcgcgt gacgcccaac
      721 gcaccctcgt atttccatgg ttggtcgccg gacgcgaagt ggctcgttta taccggaggc
      781 cgcaaacaca cgccggacgc gaagtcggac aaatacgata tctacaaaat ttccgcagac
      841 ggcggagaag agatccggct gaccgacgcg ccaggattga gcgacggccc ggagttttcg
      901 cccgatggaa aatatattta cttcaactcc acgcgcagcg ggttgatgca gatctggcgg
      961 atgaagccgg acggctccgg gcaagagcag cttacgaacg acgaattcaa caactggttc
     1021 ccgcacatct cgcccgacgg gaaatggatc gtcttcatct cctatggaca ggacatcaag
     1081 ccggaggagc atccttacta caaacatgtt ta
//
LOCUS       DASPLT010001454         1019 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_56786, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001454 DASPLT010000000
VERSION     DASPLT010001454.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1019)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1019)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1019
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_56786"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1019
                     /locus_tag="VEA63_14855"
     CDS             <1..>1019
                     /locus_tag="VEA63_14855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HYD85343.1"
                     /translation="LESPLGWISRDHRKLLVVDGEVGFVSGLCVAQMWVGDPAKQIQP
                     WRDTGVEIRGPAVGDLERSFAHIWATLGTPLPDDALPPESEAPRVGDVSVRVVASEPS
                     TSGTFRLDQLVVTFARQRVWLTDAYYLGAPTYVQALITSARDGVDVRMLLPRATDLPL
                     LKVMSRVGYRPLLEAGVRIFEWNGSVLHAKTAVVDGRWSRVGSTNLNFASWMGNCELD
                     AVMEDEGFAKEMEAMYLEDLKNATEVVLEAKRKVRAHDAGAKRPHGRSKGSAGRAAAG
                     AMRFGHTLGAALTNRRVIEPVEKRLLVGIGLILLALAALFVVFPRALAYPVGLILIWF
                     ALAMI"
BASE COUNT          158 a          272 c          389 g          200 t
ORIGIN      
        1 ctcgagtcgc cgctgggttg gatttcgcgc gaccaccgca agcttctggt cgtcgacggt
       61 gaggtcggtt ttgtttccgg gttgtgcgtg gcgcaaatgt gggtcggcga tccggcgaaa
      121 cagatccagc cgtggcgcga caccggtgtg gaaattcgcg gcccggcggt cggcgacctc
      181 gagcgttcgt ttgcgcacat ctgggcgacg ctgggcacgc cgctgccgga tgacgcgctg
      241 ccgccggaaa gcgaggctcc gcgggtgggc gacgtgagcg tgcgggtcgt ggccagcgag
      301 ccgtcgactt ccggcacgtt tcgcctcgat caactcgtag tgacgtttgc gcgacaacgg
      361 gtgtggttga ccgatgcgta ttacctcggt gcgccgacgt atgtgcaggc gttgatcact
      421 tccgcgcgcg atggcgtcga cgtgcggatg ttgctgccgc gcgcgaccga tttgccgttg
      481 ttgaaagtga tgtcacgggt cggctaccgg ccgttgctcg aagcgggggt gcgaatcttc
      541 gagtggaacg gctccgtgct gcacgcgaaa acggcggtgg tggatgggcg ctggtcgcgc
      601 gtggggtcga cgaatttgaa tttcgcgagc tggatgggaa actgcgaact cgacgcggtg
      661 atggaagacg agggtttcgc gaaggagatg gaggcgatgt atctcgagga tttgaagaac
      721 gcgacggaag tggtgttgga ggcgaagcga aaagtccgcg cgcatgatgc gggtgcgaag
      781 cggcctcacg gacggagcaa gggcagcgcc gggcgcgccg cagccggagc gatgcggttt
      841 ggccacacct tgggcgcggc gctgaccaac cgccgcgtga tcgagccggt ggaaaagcgt
      901 ctgctggtag ggatcgggct gatcctgctc gcgttggcgg cgctgttcgt cgtgttcccg
      961 cgcgcgctcg cgtatccagt aggcctgatc ctgatttggt tcgcgctggc gatgatcta
//
LOCUS       DASPLT010001455         1181 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_202250, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001455 DASPLT010000000
VERSION     DASPLT010001455.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1181)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1181)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1181
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_202250"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1081
                     /locus_tag="VEA63_14860"
     CDS             <1..1081
                     /locus_tag="VEA63_14860"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HYD85344.1"
                     /translation="RKLLMHVLGDHGYLTALVGVQHLAKAPVSDVRSLGYQQFLNEDD
                     IGEDVPDAHERAARFIESRHDRPWYLSVGLDETHRDSRKGDPASGTRFSKHHAYDPAQ
                     LDARYTRAPAIYPDLPELRRDMASFAEGARRLDERYGHILAALDRSGQAENTLVIFTT
                     DHGLAWPGMKCTLTDHGLGVALIVRGPSGFRGGRVIEPMVTHLDVFPTLMELLELPAP
                     SWLQGKSLLPLLRGEVSALHDAIFAEQGWHEVAEPQRSVRTTRFKYIRRWDPVGPKAG
                     NCDEGPAKNLLAASGWFDRVLGEELLFDLYLDPMETCNRVHEPSLADTCAELRKTLAH
                     WMESSDDPLRFGQPIQPPLWTPPST"
BASE COUNT          212 a          437 c          326 g          206 t
ORIGIN      
        1 gcgcaagctg ctcatgcacg tgctcggcga tcacggctac ctcaccgcgc tcgtgggcgt
       61 tcaacatctc gccaaggccc ccgtctccga cgtccgctcg ctcggctatc agcaatttct
      121 caacgaggac gacatcggcg aagacgtgcc cgacgcccat gagcgcgccg cccgtttcat
      181 cgaatcgcgc cacgatcgcc cgtggtatct ttccgtcgga ctcgacgaaa cccatcgcga
      241 cagtcgcaag ggcgatcctg cgagcggcac ccgtttttcc aaacatcacg cctacgatcc
      301 agcccaactc gatgcgcgtt atactcgcgc gccggcgatt taccccgacc tgccggaact
      361 ccgccgcgac atggccagct tcgccgaagg cgcgcgccgg ctcgacgaac gttatggaca
      421 catcctcgct gcgctcgacc gctccggcca ggcggagaac acactcgtca ttttcaccac
      481 cgatcacggg ctcgcgtggc ccggcatgaa atgcacgctc accgaccacg gactcggcgt
      541 ggctctcatt gtgcgcggcc ccagcggttt ccgcggcggc cgcgtcatcg agccgatggt
      601 cacgcacctc gatgttttcc ccacgctgat ggagctgctc gagttgcccg ctccgtcgtg
      661 gctccagggc aaatcgctcc tcccgctcct ccgcggggaa gtgtccgcgc tccacgatgc
      721 gattttcgcc gaacagggct ggcacgaagt cgccgagccg cagcgctccg tccgcacaac
      781 gcgtttcaaa tacatccgcc gctgggatcc ggtcgggccg aaagccggca actgcgacga
      841 aggccccgcc aaaaatcttc tcgccgccag cggctggttc gatcgcgtcc tcggcgagga
      901 actgctgttc gatctctatc tcgatccgat ggaaacctgt aaccgggtcc acgagccaag
      961 cctcgctgac acgtgcgccg aactgcgcaa aacgctcgcg cactggatgg agtccagcga
     1021 tgacccgctc cgtttcggcc agccgattca gccgccgctc tggacgccgc cctccacctg
     1081 attcgctgtc ggaaaatctc cgacaaaact cactccgcgc ctatcgagtc cagacgcgca
     1141 acggcgcaaa ataattcggc ggcgtcttgc gccgcagcgt c
//
LOCUS       DASPLT010001456         1812 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_221942, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001456 DASPLT010000000
VERSION     DASPLT010001456.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1812)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1812)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1812
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_221942"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..228
                     /locus_tag="VEA63_14865"
     CDS             <1..228
                     /locus_tag="VEA63_14865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009509771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD85345.1"
                     /translation="GANYTAGYDAATFNDIMLPSAMTFSVDLGYRSKHWELRLSVKNL
                     TDEWFFTSTSGSAALIPQPGRTFTGKVTYKF"
     gene            344..>1812
                     /locus_tag="VEA63_14870"
     CDS             344..>1812
                     /locus_tag="VEA63_14870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009509775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HYD85346.1"
                     /translation="MTHTHTSRGNAARVFRSHARALAFALCASLACALPSAPASFQVE
                     EATIADIHSALLARELTATELVHKYLARIKAYNGPAVEEPNGILQPTTPVEHASGVNA
                     IITLNLRPAAREALGFDARKARSMTDLVDDDPAMPDALEAAAALDASFAATGKLVGPL
                     HGVVIVVKDQYDTFDLRTTSAADAFYANDRPPDDATFVARLRAAGAIILGKANMGEYA
                     SGIRSAFGGTTVNAYDTSRVPGGSSGGSGVATAMSFATVAIGEESGPSIRAPATYANT
                     VGISPTQELVSRDGLINQGLNTRTGPIARTVEDAARVLTVIAGYDPKDEMTAFAVGRT
                     PDQPYEHFTREKNLAGMRIGVVREYMDKRVFSDPVYHASIDRVEQAIEDLRKLGATIV
                     EGGADGLFTDAIRRYAPMLLNTSWTKLHPELFPVDADGKPTADHLATLVELAIDPSKV
                     PGSLTMRDLGTASGNSVGESRYGFNFYLAQRGDAKIKSV"
BASE COUNT          333 a          702 c          463 g          314 t
ORIGIN      
        1 ggcgccaact acaccgccgg ttacgacgcc gccacgttca acgacatcat gctgccctcc
       61 gcgatgacgt tctccgtcga cctcggctac cgcagcaaac actgggagct tcgcctctcc
      121 gtcaagaacc tgaccgacga atggttcttc acgtccacca gcggcagcgc cgcgctcatc
      181 ccgcagcccg gccggacgtt caccggcaag gtcacgtata aattctaact cattttttgg
      241 tttgggtaat cagcaacaca aaggcgcggc gaaagctgcg ccttttttcc gccgctctcc
      301 gcgcgctcac cttcgcccgc tttcccgtcg tctcgatttc gccatgactc atactcacac
      361 ctcccgcggt aacgccgctc gcgttttccg ctctcacgca cgtgcactcg cgttcgcact
      421 ctgcgcttcc ctcgcctgtg ctctgccgtc tgcgcccgcc tccttccaag tcgaggaagc
      481 gacgatcgcc gacatccact ccgccctcct cgcccgcgaa ctcaccgcca ccgaactcgt
      541 tcataaatat ctcgcccgca tcaaagccta caacggtccc gccgtcgaag aacccaacgg
      601 tatcctgcaa cccactacgc cggtcgagca cgcgagcggc gtcaacgcga tcatcacgct
      661 caacctccga cccgccgcgc gtgaagcgct cggcttcgac gctcgcaaag cccgtagcat
      721 gaccgatctc gtcgacgacg atcccgccat gccggacgcc ctcgaggccg ccgctgcgct
      781 cgacgcctcg ttcgccgcga ccggcaaact cgtcggcccg ctccacggcg tcgtcatcgt
      841 cgtgaaggat caatacgaca cgttcgatct gcgcacgacc tccgccgccg acgccttcta
      901 cgccaacgac cgcccacccg atgacgcgac cttcgtcgcg cgcctccgtg ctgccggcgc
      961 gatcattctc ggcaaagcca acatgggcga atacgcctcc ggcattcgca gcgccttcgg
     1021 aggcaccacc gtcaacgctt acgacacctc gcgcgttccc ggcggctcga gcggcggctc
     1081 cggcgtcgcc accgcgatga gtttcgcgac cgtcgccatc ggcgaggaat ccggcccgtc
     1141 gatccgcgcc cccgccacct acgcgaacac cgttggcatt tcgcccacgc aggaactcgt
     1201 cagccgcgac ggtctgatca accagggtct caatacccgc accggcccga tcgcacgcac
     1261 cgtcgaagac gccgcccgcg tgctcaccgt catcgccggc tacgatccga aggacgaaat
     1321 gaccgcgttc gccgtcggcc gcacgcccga tcagccgtat gaacatttca cgcgcgaaaa
     1381 aaacctcgcc ggcatgcgca tcggcgtcgt ccgcgaatac atggataaac gcgtcttctc
     1441 cgatcccgtt tatcacgcca gcatcgatcg cgtcgaacaa gccatcgagg acctccgcaa
     1501 gcttggcgcg acgatcgtcg aaggcggcgc cgacggcttg ttcaccgacg cgattcgccg
     1561 ctatgcgccg atgctgctca acacctcctg gacgaagttg catccggaac tgtttcccgt
     1621 ggacgccgac ggcaagccta ccgccgatca tctcgccact ttggtcgagc tcgcgatcga
     1681 tccgtcgaaa gttccgggct cgctcacgat gcgcgacctc ggcaccgcca gcggcaactc
     1741 cgtcggcgag agccgctacg gtttcaactt ctacctcgcg caacgcggcg atgcgaaaat
     1801 caaatcggtc aa
//
LOCUS       DASPLT010001457         1519 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_98280, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001457 DASPLT010000000
VERSION     DASPLT010001457.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1519)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1519)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1519
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_98280"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1488)
                     /locus_tag="VEA63_14875"
     CDS             complement(<1..1488)
                     /locus_tag="VEA63_14875"
                     /inference="COORDINATES: protein motif:HMM:NF014218.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS1 family nucleobase:cation symporter-1"
                     /protein_id="HYD85347.1"
                     /translation="MSSPLISDRAPDASDELTASHLYNADLAPVPIGQRRWRTGSFAA
                     LWISMAACIPTYMLASSLIGGGMNWWQAVLTIFLGNVIVVIPMILNAHAGTKYGIPFP
                     VFCRASFGVTGANIPALLRAFVACGWFGIQTWIGGHAIYKILAVFWPALAAPGAATFL
                     GITFPEFACFLFFWAINMAVVYKGIDSIRFLLNLKAPLLIALGLVLLWWAHDRAGGFG
                     PILSQPSAFDAGQPKAGQFWPFFFPALTGMIGFWATLSLNIPDFSRYAVTQRAQIAGQ
                     ALGLPLTMALYSFIGVAVTSATTIIYGTTIWDPVEVLTRFQDPFVLVISMFALCLATL
                     ATNIAANVVSPANDFAHLAPKKISFRIGGLITGLVGILMMPWKLVADPTGYIFTWLIG
                     YSALLGPIGGIMIADYFVWRKRELSLPALYSGTGEYSYHRGFSWVAIAALLAGALPSL
                     PGFLATVQLIDAAHVPPFLLSLYHYAWFIGFGIAFVLYLVLRKLGP"
BASE COUNT          364 a          387 c          555 g          213 t
ORIGIN      
        1 cgggccgagc ttgcggagaa cgaggtagag cacgaaggcg atgccgaaac cgatgaacca
       61 ggcgtagtgg tagaggctca gcagaaatgg gggaacgtgc gcggcgtcga tgagttgaac
      121 ggtcgcgaga aaacccggca gactcggtag cgcgccagcg aggagtgcgg caatggcgac
      181 ccaactgaaa ccacgatgat aactgtattc gcccgtgccg gagtagagcg cggggagcga
      241 gagctcgcgt tttctccaga cgaaataatc ggcgatcatg atgccgccaa tcgggcccag
      301 cagcgcgctg tagccgatga gccaggtgaa aatgtagccg gtgggatccg cgacgagttt
      361 ccacggcatc atcaaaatgc cgaccaagcc ggtgatgagg ccgccgatgc ggaaggagat
      421 ttttttcggg gcgaggtgag cgaagtcgtt ggccgggctg acgacgttgg cggcgatgtt
      481 cgtcgcaagc gtcgcgaggc agagtgcaaa catcgagatg accaggacaa acggatcctg
      541 gaaacgggta agcacctcga cgggatccca gatggtggtg ccgtagatga tcgtggtcgc
      601 ggaggtgacc gcgactccaa taaatgaata cagcgccatc gtgagtggca ggccgagcgc
      661 ctggccggcg atttgagcgc gttgcgtgac ggcgtaacgc gagaagtcgg ggatgttgag
      721 cgacagcgtt gcccaaaagc cgatcatgcc ggtgagcgcg ggaaagaaga agggccagaa
      781 ctggccggcc ttgggctggc cggcgtcgaa ggcggacggt tgcgagagga tcggaccgaa
      841 accaccggcg cggtcatgcg cccaccaaag caggaccagg ccgagcgcga tgagcagcgg
      901 ggctttgaga ttgaggagaa agcggatcga atcgatgccc ttgtaaacga ccgccatgtt
      961 gatcgcccaa aaaaacagga agcaggcgaa ctcggggaaa gtgatgccga ggaaggttgc
     1021 ggcaccgggc gctgcgagcg caggccagaa gacggcgagg attttgtaga tcgcgtgccc
     1081 gccgatccac gtctggatgc cgaaccagcc gcaggcgacg aacgcgcgaa gcagcgccgg
     1141 aatgttcgcg ccggtgacgc cgaacgaggc gcggcaaaac actgggaagg gaatgccata
     1201 tttggtgccg gcgtgggcgt tgaggatcat cggaatcacg acgatcacgt tgcccaaaaa
     1261 aatcgtgagc acggcctgcc accagttcat gccgccgccg atgagcgacg aggcgagcat
     1321 gtaagtgggg atgcaagccg ccatggaaat ccagagcgcg gcgaagctgc cggtgcgcca
     1381 gcggcgctgg ccaatgggaa cgggcgcgag gtcggcgttg tagagatgcg acgcggtgag
     1441 ttcgtcggaa gcgtcgggag cgcggtcgga gatcagggga gaagacatgg aggggaaaag
     1501 ctccaagcac caagatcca
//
LOCUS       DASPLT010001458         2039 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_304791, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001458 DASPLT010000000
VERSION     DASPLT010001458.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 2039)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2039)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2039
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_304791"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            118..1098
                     /locus_tag="VEA63_14880"
     CDS             118..1098
                     /locus_tag="VEA63_14880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007360343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exonuclease H3TH domain-containing
                     protein"
                     /protein_id="HYD85348.1"
                     /translation="MAKWLLVDGYNLAYRCFFAIPELTRADGFPTNALHGWVKSLWML
                     ADQTKPDACVVFFDLGGPQDRLALHPDYKAQREEMPEALEKQLPYVRKLTKAMGCVGV
                     EQDGVESDDLLAAQAVALARDGHEVLIVSSDKDFAQIVNDRIKIMLPPPSANPKIGWR
                     MMDAARVQEKFGVPPEKIADYLALVGDTSDNIPGLAGVGPKTAAKWLNEYGSLEGVIA
                     NCTQLKPERFQAAICAEVEKLRRNVKLTTLNLALPTISVPPVKPHPDELYALLEELGM
                     KTALADARRRLGGPAPAPLGAVPVSAASATAVVDTRTQPPAPKPTQGELF"
     gene            complement(1248..2000)
                     /locus_tag="VEA63_14885"
     CDS             complement(1248..2000)
                     /locus_tag="VEA63_14885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HYD85349.1"
                     /translation="MRAEPKPLVVVVEDEVELANLIATHLERAGMQTQVCNRAAHAFR
                     FLKQNFANLLLLDISLPDSSGFALLEELKAADITVPVIFVTGEDQETSKIRGLDAGGD
                     DYITKPFSYDELTARIRAVLRRAESKADLNLTKNVRVGDQPFDFCGAKIIPDRLEISF
                     PDGVVQKLGRKELGILAYLNANQGSVITRKALIHSVWGIHADVRSRSLDQYIVKVREL
                     FKEHGLDLSTFRTVHGIGYIFDPAGVSKEGRE"
BASE COUNT          372 a          543 c          732 g          392 t
ORIGIN      
        1 acgggtcgtg acgcgcgctt gtttcgccgc gccctagcaa attcgcaggc ggagcttccg
       61 ttcattgttt ttcgcggccc acttgacccg gcgcttaggc gcggctctgc tgcgcgtatg
      121 gcgaaatggc tgctcgtcga cggatacaat ctcgcttatc ggtgcttctt cgcgatcccc
      181 gagctgacgc gtgcggacgg ttttccgacg aacgcgctgc atggctgggt aaagtcgttg
      241 tggatgctcg cggatcaaac aaagccggac gcgtgcgtgg tgttcttcga tctcggtggg
      301 ccgcaggacc ggctggcgtt gcatccggac tacaaggcgc agcgcgaaga gatgccggag
      361 gcgctggaga aacaactgcc gtatgtgcgc aagttgacga aggccatggg ctgcgtcggc
      421 gtggagcagg acggcgtgga aagcgacgat ttgctggcgg cgcaggcggt ggcgctcgcg
      481 cgcgacgggc acgaggtgtt gatcgtgagc tcggacaagg atttcgcgca gatcgtcaac
      541 gatcggatca agatcatgct gccgccgccg tcagcgaacc cgaagatcgg ctggcggatg
      601 atggatgcgg cccgcgtgca ggaaaagttc ggtgtgccgc cggagaagat tgccgactat
      661 ctcgcgctgg tcggcgacac gtcggataac attccgggac tcgccggcgt cggtccgaag
      721 acggcggcga agtggttaaa tgaatacggt tcgctggaag gcgtgatcgc gaactgcacg
      781 cagttgaagc cggagcgctt tcaagcggcg atctgtgcgg aagtggaaaa gctgcggcgc
      841 aacgtgaagc tcacgacgct caatctcgcg ttgccgacaa tttcggtgcc gccggtgaag
      901 ccgcatccgg acgagttgta tgccttgttg gaagagctcg gcatgaagac ggcgctggcg
      961 gatgcgcgtc ggcggttggg tgggccggcg ccggcaccgc tgggtgcggt gccggtgagt
     1021 gcggcgagcg cgaccgcggt ggtggacacg cgaactcagc ctccggcgcc gaagccgacg
     1081 cagggcgagt tgttttgagc gcgtggcggt gcggtcgaag gtggaacccg acgtcccgtc
     1141 gggttggttt gcgagtgagc gctgaatagc ccgacgggac gtcgggctcc accttcggga
     1201 agtcagccgg ggcgtaaagc cccctcccac agatgagctg aacggaacta ctcgcggcct
     1261 tctttggaca cgccggcggg gtcgaagatg tagccgatgc cgtgcacggt gcggaaggtc
     1321 gagagatcga ggccgtgctc cttgaagagc tcgcgaactt tcacgatgta ttgatcgagc
     1381 gagcggctgc ggacgtcggc gtggatgccc cacacggaat ggatcagcgc tttgcgcgtg
     1441 atgacggagc cctggttcgc attgaggtag gcgagaatgc cgagctcctt gcgcccgagt
     1501 ttttggacga cgccatcggg gaacgaaatc tcaagccggt cgggaatgat cttcgcgccg
     1561 cagaaatcga acggctgatc gccgacgcgc acgtttttcg tgaggttgag atcggccttc
     1621 gattcggcgc ggcggaggac ggcgcggata cgcgcggtga gctcgtcgta actaaacggc
     1681 ttggtgatat agtcgtcgcc gccggcgtcg aggccgcgga tcttgctggt ttcctggtct
     1741 tcgccggtga caaaaatcac cggcacggtg atatcggcgg ctttcagttc ctcgagcagc
     1801 gcgaagccgc tgctgtcggg gaggctgatg tcgaggagga ggaggttggc gaaattctgt
     1861 ttaagaaagc gaaacgcgtg ggcagcgcga ttgcacacct gtgtttgcat gccagctcgc
     1921 tccaggtgag tggcaatgag gttggccagt tcgacttcgt cttcaactac gacaacaagc
     1981 ggtttaggtt cagcacgcat ggggggaccg cagagcgatt gcagggtaat taggtttgc
//
LOCUS       DASPLT010001459         1336 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_56903, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001459 DASPLT010000000
VERSION     DASPLT010001459.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1336)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1336)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1336
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_56903"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..392
                     /locus_tag="VEA63_14890"
     CDS             <1..392
                     /locus_tag="VEA63_14890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD85350.1"
                     /translation="EDSAILYFAWDKTRVPLQLEFDVAATLVPQIEAVMASNAPKKPY
                     FQAGMYYFERDIDLKKAATWIDAAIAEQPNAFYMVYHKARLLAKTGDKAGAKAAAEKS
                     VELAKQAGGSVAEEYTRLNNALLASLK"
     gene            656..>1336
                     /locus_tag="VEA63_14895"
     CDS             656..>1336
                     /locus_tag="VEA63_14895"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HYD85351.1"
                     /translation="MLPCLRRSAAVLTGVLAFVNPLAVAQVPAPPASPEAIAHELRSF
                     STLGTLLHLAAHPDDENTQLITYFARGRGYRTAYLSLTRGDGGQNELGRDFDERLGLA
                     RTQELLAARQLDGGRQFFTRAIDFGYSKNPEETLRFWDREQVLADVVRVIRQFRPDVI
                     VTRFPIPPGSGGHGHHTASAILAVEAFKIAGDPDAYPEQLAQGLAPWQPKRVVWNVFT
                     GGRGTGGLE"
BASE COUNT          235 a          533 c          333 g          235 t
ORIGIN      
        1 gcgaagattc cgccatcctc tacttcgcct gggataaaac ccgcgtgccg ctccaactcg
       61 aattcgacgt cgccgccacg ctcgtgccgc aaatcgaagc cgtgatggcc tccaacgcgc
      121 ccaagaaacc ctacttccaa gcgggcatgt attacttcga acgcgacatc gatctgaaga
      181 aagccgccac ctggatcgac gccgcgatcg cagagcagcc caacgctttc tacatggtct
      241 atcacaaagc ccgcctcctc gccaaaaccg gcgacaaggc cggcgcgaaa gccgccgccg
      301 aaaaatccgt cgaactcgcc aagcaagccg gcggctccgt cgccgaggaa tacacgcgcc
      361 tcaacaacgc gctcctcgct tcgctgaagt aaaccgcgaa cgcactcggg gcgggccgct
      421 ccggcccgcc cttgtcgttc gccgcccgtc cgcacgccga gagatgtcat gcgctcgtct
      481 gcggcgaaac tcttcccgct agcctgcgtc tgctcttttc gagcccaccc gctcgccccc
      541 gtttccctct cgcgatgact ctgcttcgat caccctttca ccgcttttcc acttcccacc
      601 acgcgtgttc gccggttcgc ccgctcgtcg ccatccatct cgcctgatcg ccgtcatgct
      661 cccctgtctc cggcgttccg ccgcggtcct caccggcgtc ctcgctttcg ttaacccgct
      721 cgccgtcgcg caagtgcctg cgccgcccgc ctcacccgaa gccatcgcgc acgagctgcg
      781 cagcttctcc acgctcggca ctctccttca cctcgccgcg catcccgacg acgaaaacac
      841 gcagctcatc acctacttcg cgcgcggtcg cggctatcgc accgcctatc tctcactcac
      901 gcgcggcgac ggcggacaaa acgaactcgg ccgcgatttc gacgaacgcc tcggcctcgc
      961 ccgcacccag gaactgctcg ccgcgcgcca actcgacggc ggccgccagt tcttcacccg
     1021 cgcgatcgat ttcggttact cgaaaaatcc cgaggaaacc cttcgcttct gggaccgtga
     1081 acaagttctc gccgatgtcg ttcgcgtcat ccgccagttt cgccccgacg tcattgtcac
     1141 acgttttcca ataccgcccg gcagcggcgg acacggacac cacaccgcct ccgccatcct
     1201 cgcggtcgaa gcgttcaaga tcgccggcga ccccgacgca tatcccgagc aactcgccca
     1261 aggcctcgcg ccctggcaac cgaagcgggt cgtttggaat gttttcaccg ggggccgggg
     1321 caccggcgga ctcgaa
//
LOCUS       DASPLT010001460         3029 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_241774, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001460 DASPLT010000000
VERSION     DASPLT010001460.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 3029)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3029)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3029
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_241774"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..610)
                     /locus_tag="VEA63_14900"
     CDS             complement(<1..610)
                     /locus_tag="VEA63_14900"
                     /inference="COORDINATES: protein motif:HMM:NF017745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 family protein"
                     /protein_id="HYD85352.1"
                     /translation="MRLSRSAALFAAAFLCAAAFVFGASDKAADFSALLERYYEDYLA
                     LFPVDAAGSGDSDRRYDHVWQNEISAESRTAEARLAANYLTALAQFDRAALSASEQLS
                     YDVQKWSLEVRQAGLAVPTHLLPVNQFWGPPLLFAQMGSGASLHRFETEKDFRNFIAR
                     ADGFSAWVDTAIANMREGIARGLVQPRILMERVLPQYEPLMVD"
     gene            complement(663..890)
                     /locus_tag="VEA63_14905"
     CDS             complement(663..890)
                     /locus_tag="VEA63_14905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypA"
                     /protein_id="HYD85353.1"
                     /translation="MFDLSTATVIYCAFVAIVFLGLWLWYDRRDHLGFEQQRRKTTFH
                     CIRCDELYTAPVGTELAKCPNCGHENTRLKF"
     gene            complement(1214..1582)
                     /gene="rplQ"
                     /locus_tag="VEA63_14910"
                     /pseudo
     CDS             complement(1214..1582)
                     /gene="rplQ"
                     /locus_tag="VEA63_14910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017042856.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
     gene            complement(1769..2770)
                     /locus_tag="VEA63_14915"
     CDS             complement(1769..2770)
                     /locus_tag="VEA63_14915"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HYD85354.1"
                     /translation="MPKRLGKFELPSKLTKVEEGATPTYAKFIAEPFEAGYGHTVGNS
                     LRRVLLSSVEGSAISSIKIDGVNHEFQSIDGVVEDVTDIVLNLKKVLIVSTKRDAITL
                     GIRANKSGVVTAADIQADSNITIVNPDQVICTLDSKKTFDAEIEIKTGRGYYPGEANK
                     KEEQAIGVIPIDSLFSPVRLVKYSVEATRVGQITDYDKLVLEVWTDGRITPDDALKQA
                     ASILKHHLDVFDRVSEETYEFENQQSEVSEEQNKLRKLLNMSVNEIELSVRAANCLNN
                     ANITTVGELAMKTEQEMLKYRNFGKKSLNEIKEKLEALGLSLGMKFDERLLDTKKEG"
     gene            complement(2826..>3029)
                     /locus_tag="VEA63_14920"
     CDS             complement(2826..>3029)
                     /locus_tag="VEA63_14920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HYD85355.1"
                     /translation="DEIEVKNSAASRQLATRCLEENRIRNVPGWLTMNAEAFKAVVNR
                     LPNRDEMEQGINEQLIVEFYSRF"
BASE COUNT          546 a          806 c         1038 g          639 t
ORIGIN      
        1 cgtcgaccat gagcggctca tattgcggaa ggacgcgttc catgaggatg cggggctgca
       61 cgagcccgcg agcgatgcct tcgcgcatgt tggcgatggc ggtgtcgacc caagccgaaa
      121 agccgtcggc gcgcgcgatg aagttgcgga agtctttctc agtttcgaag cgatgcagac
      181 tcgcgccgga acccatctgg gcgaagagca gcggcggacc ccaaaattgg ttcaccggca
      241 gcagatgcgt cggcacggcc aaacctgcct ggcggacctc gagcgaccat ttttggacat
      301 cgtagctcaa ctgctcggag gcggagagtg cggcgcgatc gaactgcgcc aacgccgtga
      361 gatagttggc ggcaagtcgg gcctcagcgg tgcggctttc ggcgctgatt tcgttttgcc
      421 aaacgtgatc gtagcgccga tcgctgtcgc cgctgccggc ggcgtcgacg ggaaacaacg
      481 cgaggtagtc ctcgtagtag cgctcaagca gcgcggagaa atcggcggcc ttgtcggagg
      541 caccaaaaac gaaagccgca gcgcaaagaa acgcggcggc aaaaagagca gcggaacggg
      601 agagacgcat gatgacgaac ggtggtaggg agccacccac gctgttcacg tctggcctgc
      661 cgtcaaaatt tgagacgtgt gttctcgtgt ccgcagttgg gacatttggc gagttcggtg
      721 ccgaccggtg cggtgtagag ctcatcacag cggatgcagt gaaacgtggt ctttcggcgc
      781 tgttgttcga agcccaagtg atcgcggcga tcataccaga gccagaggcc gaggaacacg
      841 atcgcgacga aggcgcagta gatgacagtg gcggtggaga gatcgaacat gcgagggcgc
      901 ggcgggagcg atccggaagg tagcgggcgt catcgcggcc gccatccgaa aggagaacag
      961 cggacgaccg ccgcgaccaa aagaaaacgc gccgacctgg cggtcggcgc gtcgaaaagg
     1021 aggcgagcgg aaataacccg gcggggacgc cgggttccat ctcacgatct tacttctgct
     1081 cggtggcggg cgcttcggtc gtcgcggcgg cggcggcttc ggcggtgggc gtggctttcg
     1141 ccttcttgcc cttggccttg gtcgctttct tgcccttcga cttcttgtag ccctgatcgt
     1201 cggccttcac gaactcgatc aacgccatct cggcggcgtc gctgatgcgg cgctggccaa
     1261 gcttgtagat gcgggtgtag ccgccgttgc ggttctcgaa ttccttcacc ttctcgttga
     1321 aaagaagcgt gaccgcggtc tcatcgcgga cgtcggcaag cgcgagacgg cgcaggtgga
     1381 tcgcgtcctt cttctcggtc ttcgccgcgg ctttcttggc cttggtgatg actttctcga
     1441 tgaaggggcg caaggccttg gctttgacca gtgtggtctc aatgcggccg tgcgtgatga
     1501 gcgaggcggc catgttggag agcatcgcgc ggcgatgctc gacggtgacg ccgagggagg
     1561 cgtggtgttt attgtgacgc attgtgtttt tggttttggc gtctcccgat cggcgatccg
     1621 gtcgcagcgt ggcgcggcag cggacggcgc gagagacgag aattgaaagc tccaaatccc
     1681 aagctccaag caccaagggt gctggagact aggaacggag attggatctt ggtgcttgga
     1741 tcttggcgtt tgctgcgcgg agcgcggctc agccttcctt cttcgtgtcg aggaggcgct
     1801 cgtcgaactt catgcccagc gacaggccga gcgcttcgag cttttccttg atttcgttga
     1861 gggacttttt gccgaagttg cgatacttga gcatctcctg ctcggtcttc atcgcgagct
     1921 cgccgacggt ggtgatgttc gcgttgttca agcaattcgc cgcgcggacg gagagctcga
     1981 tttcgttgac cgacatgttg aggagcttgc ggagcttgtt ctgctcctcg ctgacctcgg
     2041 actgctggtt ctcgaactcg taggtctctt cgctgacgcg atcgaagaca tcgaggtgat
     2101 gcttgaggat cgaggcggcc tgcttgaggg cgtcgtccgg cgtgatgcgg ccatcggtcc
     2161 agacttcgag gacgagcttg tcgtaatcgg tgatctggcc gacgcgggtc gcttcgacgg
     2221 aatacttcac gaggcgaacg ggcgagaaga gcgagtcgat cggaatcacg ccgatcgcct
     2281 gttcttcctt tttgttcgct tcgccgggat agtagccgcg gccggtcttg atctcgatct
     2341 cggcgtcgaa ggtcttcttc gagtcgaggg tgcagatgac ctggtcgggg ttgacgatcg
     2401 tgatgttgga gtccgcctgg atgtcggcgg cggtgacgac gccagatttg ttggcacgga
     2461 tcccgagggt gatcgcgtcg cgtttggtcg agacaatgag aaccttcttc aggttgagca
     2521 cgatgtccgt gacatcctcg acgacgccgt cgatggactg gaattcgtga ttcacgccat
     2581 caatcttgat ggaggaaatg gcggagcctt cgaccgagct gagaaggacg cgacggagcg
     2641 aattgccgac ggtgtgaccg tagccggcct cgaaaggctc agcgatgaat ttggcgtagg
     2701 ttggcgtggc gccttcctcg accttcgtca gtttggacgg gagttcgaac tttccgagac
     2761 gtttgggcat ggcggtttgg gcggacgttg agaattaaat gaggcgagta acggggagcg
     2821 gctgcttaga agcgcgagta gaactcgacg atcagctgct cgttgatgcc ctgctccatc
     2881 tcgtcgcggt tcgggaggcg attgacgacg gccttgaagg cttcggcgtt catcgtgagc
     2941 cagccgggga cgttgcggat gcgattttcc tccaggcaac gtgtggcgag ctggcgggaa
     3001 gccgcggaat tcttaacctc gatttcgtc
//
LOCUS       DASPLT010001461         2678 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_160630, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001461 DASPLT010000000
VERSION     DASPLT010001461.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 2678)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2678)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2678
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_160630"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2020
                     /locus_tag="VEA63_14925"
     CDS             <1..2020
                     /locus_tag="VEA63_14925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD85356.1"
                     /translation="KTGTLENTAPYALVGDTDGNYTAWKPAVGSHTVRAVPYSGKNRS
                     GKAGTAKEITMKVVASVTLPDPLPETDPSDDTDPGTSNPPSQSTGLQLYKPNGQLDLS
                     LYGLLALSYDKATMEMRLGPSFLDLSPNAPDTRPTLFRPVSHRRVNGYARTNPYELGG
                     PPKTDGDYWSESGQVAYVPDHTSDPGLDRVQVYAYYDRVFAISPRLDWASGRPHPDLQ
                     TTDGHYVKLFGEAPKQPIAMVRNYGMQTNEALVLYRDGYLAVAGTQTSRAGNERPYPG
                     FVFPANKVPTNIAITTANEFALVTIWDTDTKKGQLAVFALEGKYLPFHTWPYIAMPNQ
                     GSWSAFKLLGYVDVPMAMPTSVATASNGFWNGPSQTAGRVLSQIDLSKDGDRKNVYNG
                     SWSGVVAKNGYVIVASKYDNTVALVDLTPLFSYVRESYLSSSTSFKQTMANRGTGASN
                     FPEAFSVKPSIKPTVVWQTTIAKPTAVLAGHRMDRWTKDYYKAYVASEDGTISIIDTS
                     SLMKRYSWEKLGAPRIAGTFNVGRNPVDMVFARRGDSDLPLLPYSSSGVQSRPDPYNN
                     LFYIAVRGDRKVVAAVTYQGQGAVYRTITDKRMGDPVAISTAIRGPILSVTDFRGKKL
                     ISFRIGAIDDARNKKVYGAGADGKAEFEYAGEVSFDGYPFLINTTNVN"
     gene            complement(2487..>2678)
                     /locus_tag="VEA63_14930"
     CDS             complement(2487..>2678)
                     /locus_tag="VEA63_14930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009511671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine 2,3-aminomutase"
                     /protein_id="HYD85357.1"
                     /translation="APGGGGKVPINPDYVEQITDDEVIFRNYEGKVFRYPLKSTPVVP
                     TMPEVGVQPEPVAQPIFKS"
BASE COUNT          568 a          970 c          676 g          464 t
ORIGIN      
        1 caaaaccggc acgctcgaga acaccgctcc ttacgcactc gtcggtgaca ccgacggtaa
       61 ctacaccgcg tggaaaccgg ccgtcggatc tcacaccgtt cgcgctgttc cctactccgg
      121 caaaaatcgc agcggcaaag ccggcaccgc gaaagagatc acgatgaaag tcgtcgccag
      181 cgttaccttg cccgatccgc tgcccgagac cgatccttcc gacgacaccg atcccggcac
      241 gtcgaatcct ccgtcccaat ccactggcct gcaactctac aagccgaacg gtcagctcga
      301 cctctcgctc tacggcctcc tcgcgctcag ctacgacaaa gccacgatgg aaatgcgtct
      361 cgggccgagc ttcctcgatc tcagccctaa cgcgcccgac acgcgtccga cgctgttccg
      421 tcccgtctcg cacaggcgcg tcaacggtta cgcccgcacg aatccttacg aactcggcgg
      481 cccgccgaag accgacggcg actactggag cgaaagcggc caggtcgctt acgttcccga
      541 tcacacgagc gatcccggcc tcgatcgcgt gcaagtttac gcttactacg accgcgtgtt
      601 cgccatcagc ccgcgtctcg attgggccag cggccgcccg catcccgacc tgcagaccac
      661 cgatggtcac tatgtgaaac tcttcggcga agcgcccaag cagcccatcg cgatggtgcg
      721 caactatggc atgcagacca acgaagcgct cgtgctctac cgcgatggct atctcgccgt
      781 cgcaggcacg cagaccagcc gcgccggcaa cgaacgtccg tatcctggtt tcgttttccc
      841 ggcgaacaag gtcccgacca acatcgccat caccaccgcc aacgagttcg cactcgtcac
      901 gatctgggac accgacacga agaagggcca gctcgccgtc ttcgcactcg aaggcaaata
      961 cctgcccttc cacacctggc cttacatcgc catgccgaac caaggcagct ggtccgcctt
     1021 caagctcctc ggttacgtcg acgtgccgat ggccatgccg acctccgtcg ccaccgcgag
     1081 caacggtttc tggaacggtc cgtcgcaaac cgccggtcgc gttctcagcc agatcgatct
     1141 gtcgaaagac ggcgatcgca agaacgtcta caacggctct tggtccggcg tcgtcgccaa
     1201 aaacggttac gtcatcgtcg cctccaaata cgacaacacc gtcgcactcg tcgacctcac
     1261 gccgctgttc tcctacgttc gcgagagcta cctgagctcc agcacgagct tcaagcagac
     1321 gatggccaac cgtggcaccg gcgcctccaa cttccccgag gccttctcgg tcaagccgtc
     1381 catcaaaccc accgtcgtgt ggcagaccac catcgccaag ccgaccgccg ttctcgccgg
     1441 tcaccgcatg gatcgttgga cgaaggacta ctacaaggcc tacgtcgctt cggaagacgg
     1501 cacgatcagc atcatcgata cctcgtccct catgaagcgc tactcgtggg aaaagctcgg
     1561 tgcgccgcgc atcgccggca cgttcaacgt cggtcgcaat cccgtcgaca tggtcttcgc
     1621 ccgccgcggc gacagcgatc tgccgctgct gccttacagc tcctccggcg tgcaatcccg
     1681 ccccgatccg tataacaact tgttctacat cgccgtccgc ggcgatcgga aggtcgtcgc
     1741 cgccgtgact tatcaaggcc agggcgcggt ctatcgcacg atcaccgaca agcgcatggg
     1801 cgaccccgtc gcgatcagca ccgccatccg cggcccgatc ctttctgtca ccgacttccg
     1861 cggcaaaaag ctgatcagct tccgcatcgg cgcgatcgat gacgcccgca acaagaaggt
     1921 ctacggcgcc ggcgccgacg gcaaagccga gttcgaatac gccggcgagg tctccttcga
     1981 cggttaccct ttcctgatca acaccaccaa cgtcaactga tcgcgggtcc aactcatatc
     2041 aagcgatgat agcggcgtgc cagagcacgc ctgacaggaa tcaccggcgg ccctcacagg
     2101 ccgccggttt ttttttcgac gtccgatcgc cggcccgaac tgtggaggaa ctttacgccc
     2161 cgactcgcgc cgcatccgcc tccccccgcg cgcacgtcat cagctcttaa aacacaagag
     2221 ccgccccctt tttcaagggg gcggctcaag tgacctaaca ccaagcaaac cgtaagaact
     2281 acaaacattc accgtccctg tggacgatgt tgactacacg gagatagacg gagcccgcgt
     2341 cagaacgctc aaaaaaaacc taagattttc gcaaaaaatc ggccaacccg gaagccgctg
     2401 aaattcagcg actaaacaac ccatcagaac cacggccggc gtcgctaccg cgccaggttt
     2461 aacgcaaaat tctacgccgc cccggctcag ctcttgaaaa tcggctgcgc caccggctcc
     2521 ggttgaacac ccacttccgg catcgtcggc accacgggcg tgctcttcag cggataacgg
     2581 aaaacctttc cttcgtagtt ccggaaaatc acttcgtcgt cggtgatctg ctccacgtaa
     2641 tcgggattga tcggcacttt gcctccgcca ccgggcgc
//
LOCUS       DASPLT010001462         4482 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_56915, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001462 DASPLT010000000
VERSION     DASPLT010001462.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 4482)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4482)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4482
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_56915"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            344..853
                     /locus_tag="VEA63_14935"
     CDS             344..853
                     /locus_tag="VEA63_14935"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002408090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HYD85358.1"
                     /translation="MPISDNVRLGEGVMIHHPQLVNLYGCSVGSGSRVGAFVEIQKNA
                     HIGERCKISSHTFICEGVEIEDEVFVGHGVMFINDIFPQAANEDGSAQNEADWAVVPT
                     RIKTRASIGSNATIMGGVTVGVGALVGAGAVVTKDVPDYAIVAGVPAKVVGDVRDKRK
                     SAAQALKQG"
     gene            859..1887
                     /locus_tag="VEA63_14940"
     CDS             859..1887
                     /locus_tag="VEA63_14940"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HYD85359.1"
                     /translation="MIRIGVIGYGYWGPNLVRNFAELKGAELAAVADLDPKKLELVNK
                     RFPAVKTTTDFQEMLRDPSIDAIAIATPVSTHFELGMAALKSGKHVWLEKPMTETSLQ
                     ARQLVDCAEKKKLVLHVDHTFIYTGAVQKMKDLVAADELGKILYYDSTRVNLGLFQRD
                     VSVISDLAVHDFSILDYLLQEQPTAVSAGGINHFPGTPENLAYITMFYDSGAIAHVNC
                     SWLAPVKVRTVLVGGTKKMFVYDDLQPSEKLKIYDKGVSFTDDPQKIYQMRVGYRTGD
                     MWAPKLDESEALRAGATDFVEAIEKKKQTLCDGRLGARVIQIIEAATSSMKARGETIH
                     LPRAERVL"
     gene            1884..3005
                     /locus_tag="VEA63_14945"
     CDS             1884..3005
                     /locus_tag="VEA63_14945"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HYD85360.1"
                     /translation="MSVPFVDLKAQYLSIKPEVDAAIQNILDNTQFILGAEVAAFEKE
                     FAEYCGAKHAMGVNNGTSALHLAMLAADIGAGDEVITTPFTFVASVAAIWYSGAKPVF
                     VDIDPKTYLIDPAKIEAAITPKTKAILPVHLYGQSCDMDAIMAVAKKHNLIVIEDACQ
                     AHGAEWNGKRVGGIGDLGAFSFYPGKNLGAYGEGGATTTNNDEYARKIRMLRDWGAEK
                     KYMHVLKGYNMRLEGMQGAILRVKLKYLEKWTEARREAAKRYDALFAGSGVETPFVQD
                     QSRHVYHIYSTRTTKRQEWQDALNAKGIQSGIHYPIAIHNLPAYEDLGYKNGDFPHAE
                     KAAAEQLSLPMYPELTPEMQAEVAAAVIELHKKDAGVAR"
     gene            3002..3970
                     /locus_tag="VEA63_14950"
     CDS             3002..3970
                     /locus_tag="VEA63_14950"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HYD85361.1"
                     /translation="MNVEGAKILVTGGCGLIGSTTIDLLLREDVQKIVIFDNLVRGSM
                     HNVAEILKDKRVELIKGDIRDVDATRRVSEGMDAVIHMAAIRITACAEHPREALEVMC
                     DGTFNVVEAAHLAGLKRIVAASSASVYGMADSFPTTEKDHPYNNRTWYGASKVMLEGL
                     LRSFNDMYGTEYCAFRYFNVYGPRMDIHGKYTEVLIRWMERIEAGQPPLILGDGKTSM
                     DFIYIDDIAAANVAGLKSDVSDEVFNVASGVETNLNELADALMRVMGKRLQPEYGPER
                     KVNAVPRRLASIEKTTRLLGWSPKVDLEEGLERLVSWWRVNKEHVA"
     gene            3967..>4482
                     /locus_tag="VEA63_14955"
     CDS             3967..>4482
                     /locus_tag="VEA63_14955"
                     /inference="COORDINATES: protein motif:HMM:NF013227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HYD85362.1"
                     /translation="MIPLMKPLMDEREAQAASRVILSGWVTQGPEVAAFEREFAEFVG
                     APHATAVSNCTTALHLALLAVGVGAGDEVITVSHSYVATANSIKYCGAEPVFVDVELD
                     TYNMDPAKIEAAITPKTKAILAVHQIGMPCDIDAIVAIGKKHGLPVIEDAACAIGSEI
                     QIDGQWERIGAP"
BASE COUNT          931 a         1344 c         1428 g          779 t
ORIGIN      
        1 gcgcggcttt gctcggcgat cgtgacgccc gcgccacgct ttccgcggcc gcgctcgcct
       61 gcgcgcgcga acatttctcg gccgctgcct gctttcgcga tctcactgcg tttgtccgcg
      121 gcgacgacgc cgcattgacg cgaaattcct tgcgaacggc gctgtcacag tcccaatagg
      181 ggcgaaattc tccctagcgt cgcaagggct tggagacgcg gttccgcttg gtcaaaagac
      241 cgctgttccg cggcttttgg tcgacgcaag acgcgcgcac gttaagcata acgcggcagg
      301 gaaattgcga cgtgaggcgc gcagttcgcg cgggggtgct gggatgccga tttcggacaa
      361 tgttcgcctg ggtgaaggtg tgatgatcca ccatccccag ctcgtgaact tgtacggctg
      421 ctcggtcggc tcgggctcgc gcgtgggcgc gttcgtggaa atccagaaga acgcgcacat
      481 cggagaacgc tgcaagatca gctcgcacac cttcatctgc gagggcgtcg agattgagga
      541 cgaagtgttc gtcggccacg gcgtcatgtt catcaacgac attttccccc aggcggcgaa
      601 cgaggacggc tcggcgcaga atgaggccga ctgggccgtg gtgccgacgc ggatcaagac
      661 gcgcgcttcg atcggctcga atgccaccat catgggcggc gtgaccgtgg gcgtcggggc
      721 gctcgtgggc gctggcgcgg tcgtgaccaa ggatgtgccg gattacgcga tcgtggccgg
      781 ggtgccggca aaggtcgtcg gggatgtgcg cgacaaacgg aaaagcgcgg cgcaagcgct
      841 gaagcaaggt taagggcgat gatcaggatt ggcgttatcg gctacggcta ttggggtcca
      901 aacctcgtcc gtaacttcgc ggagctgaaa ggcgccgagt tggcggctgt ggccgacctc
      961 gacccgaaaa agctcgaact cgtcaacaag cgcttccccg cggtgaagac gacgaccgat
     1021 tttcaggaaa tgctgcgcga tccgagcatc gacgcgatcg ccatcgcgac gccggtgtcg
     1081 acgcatttcg aactcggcat ggcggcgctt aagagcggca aacacgtctg gctggaaaag
     1141 ccgatgacgg aaacgtcgct gcaagcccgc cagctggtcg attgcgccga gaagaagaag
     1201 ctcgttctcc acgtcgacca cacgttcatt tacaccggcg ccgtgcagaa gatgaaggat
     1261 ctcgtggccg ccgatgaact gggcaagatc ctttattacg attcaacccg agtgaatctc
     1321 ggcctcttcc agcgcgacgt gagcgtcatc agcgatcttg cggtgcacga tttctcgatc
     1381 ctggattatt tgctgcaaga gcaaccgacg gcggtttcgg ccggcggtat caaccacttc
     1441 ccgggcacgc cggaaaacct cgcctacatc acgatgttct atgactctgg cgcgatcgcg
     1501 cacgtgaact gctcgtggct ggcgccggtg aaggtgcgca cggtgctcgt cggcgggacg
     1561 aagaagatgt tcgtgtatga cgatctgcag ccgtcggaga agctgaagat ctacgataaa
     1621 ggcgtctcgt tcacggacga tccgcagaaa atttaccaga tgcgcgtcgg ctatcgcacc
     1681 ggcgacatgt gggcgccgaa actcgacgaa agcgaagccc tgcgcgccgg cgcgaccgac
     1741 ttcgtcgaag cgattgaaaa gaagaagcag acgctgtgcg acggccgcct cggcgcccgc
     1801 gtcatccaga tcatcgaagc cgccacctcc tccatgaagg cccgcggcga aaccatccat
     1861 cttccccgtg cggagcgtgt actgtgagcg tccctttcgt cgatctgaaa gcccaatacc
     1921 tctcgatcaa gccggaggtg gacgcggcga tccagaacat cctggacaac acgcagttca
     1981 tcctcggcgc cgaagtggcg gcgttcgaga aggaattcgc cgaatattgc ggcgctaagc
     2041 acgcgatggg cgtcaataac ggcaccagcg cgctgcacct cgccatgctc gccgccgata
     2101 tcggcgcggg cgatgaagtg atcacgacgc cgttcacgtt cgtcgcgtcc gtggcggcga
     2161 tctggtactc gggcgcgaag cccgtcttcg tcgacatcga cccgaagacg tacctgatcg
     2221 atccggccaa aatcgaagcg gcgatcacgc cgaagaccaa ggcgatcctg ccggtccacc
     2281 tctatggtca gtcctgcgac atggacgcga tcatggcggt cgccaaaaag cacaatctga
     2341 tcgtgatcga agacgcctgc caggcgcatg gcgcggaatg gaacggcaag cgtgtcggcg
     2401 gcatcggcga tctcggcgcg ttcagcttct atccgggcaa aaacctcggc gcctatggcg
     2461 aaggcggcgc gacgacgacc aataacgacg agtatgcgcg caagatccgc atgctgcgcg
     2521 attggggcgc tgagaagaag tacatgcacg tcctcaaagg ctacaatatg cgccttgagg
     2581 gcatgcaggg cgccatcctg cgcgtgaagc tgaagtatct cgagaagtgg accgaagcgc
     2641 gccgtgaagc ggcgaagcgc tacgatgctt tgttcgcagg ttcgggcgtc gagacgccgt
     2701 tcgtgcagga ccagtcgcgg cacgtctatc acatctactc gacgcgcacg acgaagcgcc
     2761 aagagtggca ggacgcgctg aacgccaagg gcatccaatc gggcatccac tacccgatcg
     2821 cgatccacaa cctgccggcc tacgaagacc tcggctacaa gaacggcgac ttcccccacg
     2881 ctgaaaaggc ggcggcggaa cagctgtcgc taccgatgta tccagaactg acgcctgaga
     2941 tgcaggcgga agtcgcggca gcggtgatcg aactccacaa gaaggacgcg ggggtggccc
     3001 gatgaacgtc gaaggcgcaa aaatcctcgt caccggcggc tgcgggctga tcggttcaac
     3061 gacgattgat ctcttgctgc gtgaggatgt ccagaagatc gtgatcttcg acaacctcgt
     3121 acgcggctcg atgcacaatg tcgcggaaat cctgaaggac aagcgcgtcg agctgattaa
     3181 gggcgatatc cgcgacgtgg atgcgacgcg ccgcgtcagc gaaggcatgg acgcggtcat
     3241 tcacatggcg gcgatccgca tcaccgcctg cgccgagcat ccgcgcgaag ctctggaagt
     3301 gatgtgcgac ggcacgttca acgtggttga agctgcgcac ctggccggat tgaagcgcat
     3361 cgtggcggcg tcgtccgcct ccgtttacgg catggccgat agcttcccga caaccgagaa
     3421 ggatcacccg tacaataacc gcacctggta cggcgcatcg aaggtgatgc tcgaaggcct
     3481 gctgcggtcg ttcaacgaca tgtacggcac cgaatattgc gcgttccgct atttcaacgt
     3541 gtacggcccg cgcatggaca tccatggcaa gtacaccgaa gtgctgatcc gctggatgga
     3601 gcgcatcgaa gccggccagc cgccgctgat cctcggcgac ggcaagacct cgatggactt
     3661 catctatatc gacgacatcg ccgcggcgaa cgttgcgggt ttgaagtccg acgtttcgga
     3721 tgaggtgttc aacgtcgcct caggcgtcga aacgaacctc aacgaactgg ccgatgcgct
     3781 catgcgcgtg atgggcaagc ggctgcagcc ggaatacggg ccagagcgca aggtcaacgc
     3841 cgtgccgcgc cgcctggcct cgatcgaaaa gaccacacgc ctgctgggct ggtcgccgaa
     3901 ggtcgatctc gaagaaggct tggagcgtct ggtttcgtgg tggcgcgtca acaaagagca
     3961 cgtcgcatga tcccgctgat gaagccgctg atggatgaac gcgaggcgca agctgcgtcg
     4021 cgcgtgatcc tgtcgggctg ggtgacgcaa ggcccggaag tggcggcgtt cgaacgcgag
     4081 ttcgccgaat tcgtcggcgc gccccacgcg accgcggttt ccaactgcac cacggcgctg
     4141 catctggcgc tgctcgcagt tggcgtcggc gctggcgatg aagtcatcac ggtctcgcac
     4201 agctacgtgg cgacggcgaa ctcgatcaaa tattgcggcg cagagccggt gttcgtggat
     4261 gtcgaactcg acacctacaa tatggacccg gccaaaatcg aagctgcgat cacgccgaag
     4321 accaaagcga ttctcgccgt tcaccagatc ggcatgccgt gcgacatcga cgcgatcgtc
     4381 gccatcggca agaagcatgg attgccggtg atcgaagacg cagcttgcgc catcggttcg
     4441 gaaatccaga tcgacggcca gtgggagcgc atcggcgccc cg
//
LOCUS       DASPLT010001463         2477 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_56930, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001463 DASPLT010000000
VERSION     DASPLT010001463.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 2477)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2477)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2477
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_56930"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..989)
                     /locus_tag="VEA63_14960"
     CDS             complement(<1..989)
                     /locus_tag="VEA63_14960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD85363.1"
                     /translation="MSSAIILLQLAVLARTTLKRPLRITAFLLRVFVAFLAWNSCVSA
                     VEGLPVMQTFGPRETGSSGFYWCTAQDERGVLYFGTDALLTFDGDRWSQYHVPGAHAI
                     RAVAVDPRGRVWIGAVNEIGYFDRAATGGLSEYHSLVPYLSPTTAPLGNVWHAFVDEG
                     RAIFVTADQVLVWDGKEFTANFLPGTRRLPAMQADGRIFVSHLTEGLFILEGKRLRLF
                     TAAAAADERGVAWIKRDGDGWLMVTTRGLARLAGDKVFPLGDTAGDFITSNVLSSAAS
                     FPNGDICVGTLSGGLAVFSPDGALKQVLTSADGLQATSVHSVFVDRDGAAWVSS"
     gene            complement(1021..1197)
                     /locus_tag="VEA63_14965"
     CDS             complement(1021..1197)
                     /locus_tag="VEA63_14965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD85364.1"
                     /translation="MFAQLGQALGERIDAFGGLRQTNGEQGAGNQPEGDAFNRFHRRN
                     ESGLFSAATIPQTA"
     gene            1366..2259
                     /locus_tag="VEA63_14970"
     CDS             1366..2259
                     /locus_tag="VEA63_14970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD85365.1"
                     /translation="MLSLAAYRYRNILETDGGPIARVSIREFSVYGRKMFQANAWLAE
                     GVIPPRRKFALYSDADGTGTSDVPMVARHMAISEAMERWAFHSTVHAPDRELYGFDLD
                     ESSNGMAAFPGIFHGQARKRAMLEAVERASVIAWWEGLLDGEVRPTEWPDINALVLPS
                     PIGHGVTVLAFRQTRPDTFAYGHGAAQDFFGASERAVMELARHEYVLGIHRLSKNVAA
                     HEMPSDLFERRALFFSSAEGHALFQERLRRRAKGPRYQAKVVHDTEIRGPWSHYASVW
                     RVLIDPPSKDFLVDTERYFFW"
BASE COUNT          474 a          772 c          792 g          439 t
ORIGIN      
        1 gagctgaccc acgcggcgcc gtcgcgatcg acgaaaaccg aatgcacgct cgtggcctgc
       61 aagccgtcgg ccgacgtgag cacttgttta agcgcaccgt ctggcgagaa caccgcgagt
      121 ccgccgctca acgttccaac gcaaatgtcg ccattcggaa aactcgcggc cgacgaaagg
      181 acgttgctcg tgatgaaatc gccggcggtg tcgccgagcg gaaaaacttt atcgccagca
      241 agccgcgcga gtccgcgcgt cgtcaccatc aaccacccgt cgccgtcccg ttttatccaa
      301 gcgacaccgc gctcgtccgc ggcggctgcg gcggtgaaga gccgcaggcg tttgccctcg
      361 aggataaaca atccctcggt gaggtggctc acgaaaatcc ggccgtccgc ttgcatcgcg
      421 ggcagtcggc gcgtgccggg aaggaaattg gcggtgaatt ccttcccgtc ccagacgagc
      481 acctggtcag cggtgacgaa gatggcgcga ccttcgtcga cgaaggcatg ccagacattg
      541 ccgagaggcg cggtggtggg ggagaggtaa gggacgagag agtgatattc ggaaagcccg
      601 ccggtcgcgg cgcggtcgaa gtagccgatt tcgttgaccg cgccgatcca gacgcggccg
      661 cggggatcga cggccacggc gcgaatcgcg tgtgcgccgg gcacatgata ctggctccag
      721 cgatcgccgt cgaacgtcag caaagcgtcg gtgccaaaat aaagcacgcc gcgttcgtct
      781 tgcgccgtgc accagtagaa gccgctgctg ccggtttcgc gtggaccgaa cgtctgcatg
      841 accggcaaac cctccaccgc tgagacgcag gaattccacg cgagaaacgc cacgaaaaca
      901 cgcagcagga aagcggtgat acgaaggggc cttttcaacg tcgtgcgtgc gagcacggcg
      961 agctgtagca gaataattgc gctgctcact actgaaaaac acgtagcgtg gggcttaaaa
     1021 ttaagcggtt tgagggatag tggcggctga aaaaaggccc gattcgttgc ggcgatgaaa
     1081 acgattaaac gcatctccct caggctgatt tccagctccc tgctcgccat tggtctgtcg
     1141 caaaccgccg aacgcgtcga tcctctctcc caaagcctgg ccaagctgcg cgaacacggc
     1201 ggcgacctcg ccggtcccgc gctgccgtgt gcacgctcca tgggcgacat cgccggtccg
     1261 gcgctaccgt gtgcgcgcgc gagcgacact cataatcaac aggaccgtgc cctcccgtgc
     1321 gctttccaat gctgagttga gtcgcgtttc gttctctcgc tcccgatgct cagcctcgcc
     1381 gcatatcgtt accgaaatat tctggagacc gatggcggcc ccatcgcccg ggtgtccatc
     1441 cgcgagttct ccgtttacgg acggaagatg tttcaggcga atgcctggct cgcggagggt
     1501 gtgataccgc cgcggcggaa gttcgcactc tacagcgacg cggacggcac cggcacctcg
     1561 gacgtgccga tggttgcgcg gcacatggcg atctccgagg cgatggagcg ctgggcgttt
     1621 cactcgacgg tgcatgcgcc ggatcgcgaa ctctacggct tcgatctcga tgaatcgtcc
     1681 aatggcatgg cggcttttcc cggaattttc cacgggcagg cgcgcaagcg ggcgatgctt
     1741 gaagccgtgg agcgcgcgag cgtgatcgcg tggtgggaag ggctgctcga cggcgaagtg
     1801 cggccgaccg aatggccgga catcaacgcg ctcgtgctcc cgagcccgat cggccacggc
     1861 gtgaccgtgc tggcatttcg gcagacgcgg cccgacacgt tcgcctacgg gcacggcgca
     1921 gcgcaggatt tttttggcgc gagcgaacgc gccgtgatgg agctcgcgcg acacgaatac
     1981 gtgctcggca tccaccggct ctcgaaaaac gtcgccgcgc acgagatgcc ctcggacctc
     2041 ttcgagcgcc gcgcgctgtt tttctcgtcg gcggaagggc acgcgctttt tcaggaacgg
     2101 ctgcgccgac gcgcgaaagg cccgcgttat caggccaagg tggtgcacga caccgaaatc
     2161 cgcggcccct ggtcgcacta cgcatcggtc tggcgcgtgc tgatcgatcc gccgtccaaa
     2221 gattttctcg tcgatactga gcgttacttt ttctggtaag cgcgaaaaaa cagccgcacg
     2281 agcggtgtgg aacgcggagc gcgaacgcgc cgccgaaaca tgtagcggca ggcctccgtg
     2341 cctgcctgcc gcttgttgtt tgctgagctt ggaatgaccg cccggcgcat tcgactcaga
     2401 tgtccctgtg caacgggtct gataaaccaa ggatgtggcc gttgtaggag ccttcggacc
     2461 tctcgccggc tatgggt
//
LOCUS       DASPLT010001464         2176 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_160703, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001464 DASPLT010000000
VERSION     DASPLT010001464.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 2176)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2176)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2176
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_160703"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1523)
                     /locus_tag="VEA63_14975"
     CDS             complement(<1..1523)
                     /locus_tag="VEA63_14975"
                     /inference="COORDINATES: protein motif:HMM:NF015714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HYD85366.1"
                     /translation="MTSRSLGHRAPLLWIVLPMLAGLAAARVGDFATVPWLLGGAALM
                     AGAAVAASSRKPRWFPPLFCAAMFLAGAAMYGLHRPRIAAWDRLPAREARLTLIVQRV
                     YPQMDARSVAGLATIQKADTPLNELAGQRVYFSLAVRQGEAPPLRSSVVSAIGVIASL
                     PRDPSPDTFDGYLANAGINFRFSRGRILAEERPANAYYRFCDRQLTRFAKILGAGVEA
                     KRPELTAVLRAMLLGQQHELSDEQDTLFRQSGTMHVFSISGLHIAVIAGGLHAILMLL
                     RLPRPMQFVVGMTALWLYVDITGAAPSAVRAFVMVALVQASLVLRVPRNPLSALAASA
                     LLVILVAPLQVFSASFQMSYSIVAALLLMGLPLADRWNEKLALFRDLPGVTWTWHHHL
                     RDGLWRATLSALALGVSASLVSAVTSVLFFQLFTPGALLANLWLIPASSLSILIGFIS
                     LMCGLVGFAAGGVLANHAAVLTLWAIDAGVRGSVALPAAWFDATFRAPWLGNLALALL
                     "
     gene            1927..>2176
                     /locus_tag="VEA63_14980"
     CDS             1927..>2176
                     /locus_tag="VEA63_14980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD85367.1"
                     /translation="MSSADQPDLFAGEPEGAAATFSAAPAKKSAHQPLAARMRPRRLS
                     EVVGQDHILKPGSLLPRLVAQNRFGSLPRQLLMNAEIVS"
BASE COUNT          445 a          670 c          706 g          355 t
ORIGIN      
        1 agcagcgcca gtgcgaggtt gccgagccat ggcgcgcgaa acgtcgcgtc gaaccacgcg
       61 gccggcaacg ccacgctgcc gcgcacgcct gcatcgatcg cccacaatgt cagcaccgcc
      121 gcatgattcg ccagcacgcc accggccgcg aaaccgacga gcccgcacat gagcgagatg
      181 aagccgatca agatcgaaag gctcgacgcc gggatcagcc acagattcgc gagcagcgcg
      241 cccggcgtga acagttggaa gaacaacacg ctcgtcaccg cgctcacgag cgatgccgag
      301 acgccgagcg cgagcgccga caacgttgcc cgccagagtc cgtcgcgcag atgatgatgc
      361 cacgtccacg tcacgccggg gagatcgcga aaaagcgcga gtttttcatt ccagcgatcg
      421 gcgagcggca gacccatcag caacagcgcc gcgacgatgc tgtagctcat ctgaaagctt
      481 gcgctgaaca cttggagcgg cgcgacgagg atcacgagca acgccgacgc cgcgagcgcc
      541 gagagcggat tgcgcggcac gcgcagcacc aacgaggctt gcacgagtgc gaccatgaca
      601 aacgcgcgca cggcggatgg cgccgcgccc gtgatgtcga catacagcca aagggccgtc
      661 atcccgacga cgaactgcat gggtcgcggc agccgcagca gcatcaagat tgcgtgcagg
      721 ccgccggcaa tcaccgcgat gtggagcccg ctgatcgaga agacgtgcat cgtgccgctc
      781 tggcgaaaca gcgtgtcctg ttcgtcgctc agttcgtgtt gttgaccgag cagcatcgcg
      841 cgcaacaccg cggtgagttc ggggcgcttc gcttccacgc cggcgccgag gattttcgca
      901 aagcgcgtga gctgtcggtc gcagaaacgg taatatgcgt tcgcgggccg ttcctcggcg
      961 aggatgcgtc ctcgcgaaaa acgaaaattt atcccggcgt tcgccagata gccgtcgaac
     1021 gtgtcgggcg aaggatcgcg cggcaggctg gcgatgacgc cgatcgccga gacgaccgac
     1081 gaacgaagcg gcggcgcctc gccttggcgg acggccagcg agaaataaac gcgttgcccg
     1141 gcgagctcgt tgagcggcgt atccgctttt tggatcgtcg cgagtccggc gacgctccgc
     1201 gcgtccatct gcggataaac gcgctgaaca atgagcgtga gccgtgcttc gcgtgccgga
     1261 agccggtccc acgcggcgat ccgcggccgg tgaagcccat acatggccgc gccggcgaga
     1321 aacatcgcgg cgcagaacag cggaggaaac cagcgaggtt tcctcgagct cgcggcgacg
     1381 gcggcgccgg ccatgagggc ggctccgccg agcagccagg gaacggttgc aaagtcgccg
     1441 acgcgagccg cggcgagccc cgcgagcatc ggcaggacaa tccacagcaa cggcgcgcgg
     1501 tgtccgagac tgcgactggt catgcgtcgc aagtgaaacc actaaaccgc gcgaaagaac
     1561 agccatttgc agaagcgcgg cggcgtgtgg ccggatttat ttaaccacag atggccacgg
     1621 atgaacacgg attgggggca gaccaagccg gtttgaacca cgaatggaca cgaattcaca
     1681 cgaatgaaat tcgagggaag ctccttcagg cttcgtaagg gttggggtgt tgcgttcgcc
     1741 gaacagcccg acggggacct cgagctccac ctcggcctct ctattcgtgt gaattcgtgt
     1801 ccattcgtgg tttcaattca agggcgggaa tctgtgttca tcggtgttca tccgtggttt
     1861 caaatttcag ttgtcgttct gaggttttga ttcggcggaa atcgtgttca ttagtggttt
     1921 ccttcgatgt catccgctga ccaacccgat ctttttgccg gtgagcctga aggggcggct
     1981 gccacgtttt cggcggcgcc cgcgaagaaa tcggcgcatc agccgctcgc tgcgcggatg
     2041 cgtccgcggc ggttaagtga agtcgtgggg caggatcaca ttctcaaacc gggcagtctg
     2101 ctgccccggc tcgtcgcgca gaatcgattc ggatcgctgc cccgacagct tttgatgaac
     2161 gccgaaatcg tgtcgt
//
LOCUS       DASPLT010001465         1151 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_141272, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001465 DASPLT010000000
VERSION     DASPLT010001465.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1151)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1151)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1151
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_141272"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            109..>1151
                     /locus_tag="VEA63_14985"
     CDS             109..>1151
                     /locus_tag="VEA63_14985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HYD85368.1"
                     /translation="MPEIENASLSQAELLARLQHLERQETATLRELRDVKAALDEHSI
                     VAITDPSGKITYINDKFCAISKYSREELLGQDHRIINSGHHSKAFFRELWTTIAQGRV
                     WHGEIKNRAKDGTFYWVDTTIYPFLNEAGKPRQYVAIRTDITQRKANETALHELAAEL
                     AEKNKELEAIVYTVSHDLRSPLVNVQGFGKQLNRACDTIRAAVAAAENGAVPAEKLKQ
                     PLEVAIPQALRFINAGVAKMETLLAGLLRYSRLGRVALTIRPLNMNGMLAEILAAMKF
                     QLDEAKAEVRIEPLPTCLGDTVQTSQVFANLIDNALKYRDPARPLRLVIRGDVRDGQA
                     VYQIADNGLGIAA"
BASE COUNT          246 a          421 c          301 g          183 t
ORIGIN      
        1 gtccgcgctc ggtcccggca cggtcgcgtc ccgttgcgcg ccgagcgtcg cacactccga
       61 acacaccaag tcttgcgatg acgcctgccg atcccgacat tgttccccgt gccggaaatc
      121 gaaaacgcct ctctttcgca agccgagctg ctggcgcgcc tgcaacatct cgagcgccag
      181 gagaccgcca ccttgcgtga actccgcgac gtcaaggccg cgctcgacga acactccatc
      241 gtcgcgatca ccgacccgtc gggaaaaatc acttacatca acgacaagtt ctgcgcgatt
      301 tccaaatact cgcgcgaaga actcctcggc caggatcacc gcatcatcaa ttccggccac
      361 cattcgaaag catttttccg cgagctctgg actaccatcg cacaaggccg cgtctggcac
      421 ggcgagatca agaaccgcgc gaaagacggc acgttctact gggtcgacac cacgatttat
      481 ccgtttctca acgaagccgg aaaaccccgg caatacgtcg ccatccgcac cgacatcacg
      541 caacgcaagg ccaacgagac cgccctccac gaactcgccg ccgaactcgc cgaaaaaaac
      601 aaggagctcg aggcgatcgt ctacaccgtg tcgcacgacc tccgctcgcc actcgtcaac
      661 gtgcaaggct tcggcaagca gctcaaccgc gcgtgcgaca ccattcgcgc tgccgtcgcc
      721 gcggccgaga acggcgccgt tcccgccgaa aaattgaagc agccgctcga ggtcgccatc
      781 ccgcaggcgc tgcgcttcat caacgccggc gtcgcgaaaa tggaaacgtt gctcgccggc
      841 ctcttgcgtt attcgcggct cggccgcgtc gcgctcacga tccgtccgct caacatgaac
      901 ggcatgctcg cggaaattct cgcggcgatg aaattccagc tcgacgaagc gaaagccgag
      961 gtgcgcatcg agccgctgcc cacttgtctc ggcgacaccg tgcaaaccag ccaggtcttc
     1021 gccaacctca tcgacaacgc cctgaaatat cgcgatcccg cgcgtccgct gcgtctcgtc
     1081 atccgcggag acgtgcgcga cggccaggcc gtttaccaga tcgccgacaa cggcctcggc
     1141 atcgccgcgg a
//
LOCUS       DASPLT010001466         1397 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_263553, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001466 DASPLT010000000
VERSION     DASPLT010001466.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1397)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1397)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1397
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_263553"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(170..1006)
                     /locus_tag="VEA63_14990"
     CDS             complement(170..1006)
                     /locus_tag="VEA63_14990"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF013509.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HYD85369.1"
                     /translation="MKKSPPSSVASSSTSAASRFWRWLAIVSLAVNIGLAVWATRRAK
                     AADERATVVKETEAAAVSMSSAVASASIARPKPKGAYAALGSYMAENNRIADLGWSEA
                     QFAEFLDGMRASYEGRGLPLDEEAKALRDEISRRVQAMLAVEQPDPVEDYFRMLRDKE
                     GASRTESGLHYRMTETGAGPTAQATDVVVLSFAARQPDGKELPELSRARVRMKVADLL
                     PGLAEGVQRLNVGGKALMFLPASLSFSAANWPAQVPEGMPLAFFVELHEIEPSDDLGA
                     WR"
     gene            complement(1003..>1397)
                     /locus_tag="VEA63_14995"
     CDS             complement(1003..>1397)
                     /locus_tag="VEA63_14995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ASPIC/UnbV domain-containing protein"
                     /protein_id="HYD85370.1"
                     /translation="VRRDAKALATIDFNQDGWADVLVTRNNDRPLALLNGGRGGRRSF
                     GVALRGAAGNPAAVGARAMLTLADGSMQTSEVVAGSGYFAQSTATLFFGYPEGVAPEK
                     LKIRWPDGRETVHTFEAAPAKTLRFSAP"
BASE COUNT          264 a          546 c          369 g          218 t
ORIGIN      
        1 acggcaccca gaagagcgcg tgatgcacgc gagagcggat cgtggtcatg gggtagaaag
       61 ttgaagtgga aaaaaggata caatcgccga aaccgaaact cgaccactgc gcacccgccg
      121 gccaaagccc gctcgcgcaa cccgcccggc ggctcgcttc atggtggggt taacgccatg
      181 cgcccaaatc gtccgacggt tcgatctcgt ggagctccac gaaaaacgcg agcggcattc
      241 cttccggcac ctgcgccggc cagttcgccg cgctgaacga caacgacgcc ggcaggaaca
      301 tcaacgcctt cccgccgacg ttcaaccgct gcacgccctc cgcgagcccc ggcagcaaat
      361 ccgccacctt catccgcacc cgtgcgcgac tcagctccgg cagttctttc ccgtccggct
      421 gccgcgccgc aaagctcagc acgaccacat ccgtcgcctg cgccgtcgga cccgcgcccg
      481 tttccgtcat ccgataatgc agcccgctct ccgtccgcga cgcgccttcc ttgtcgcgca
      541 acattcgaaa ataatcctcc accggatccg gctgctcgac cgcgagcatc gcttgcaccc
      601 gccggctgat ctcgtcgcgc aacgccttcg cctcctcatc caacggcaac ccgcgccctt
      661 cgtagctcgc acgcatgccg tcgagaaatt ccgcaaactg cgcctcgctc cacccgagat
      721 ccgcgatccg attgttctcc gccatgtagg aaccgagcgc cgcatacgcc cccttcggct
      781 tcggccgcgc gatcgacgcc gatgccacgg ctgatgacat cgacaccgcc gccgcctccg
      841 tttctttcac gactgtcgcg cgctcgtcag ccgctttcgc ccgccgcgtc gcccacaccg
      901 ccagaccgat gttcaccgcc agcgagacga tcgccagcca ccgccaaaaa cgcgatgcag
      961 cagaggtcga agacgaggcg accgacgacg gtggagattt tttcacgggg cggagaaacg
     1021 cagcgttttc gcgggcgcag cctcgaaggt gtgcaccgtt tcgcgcccat caggccagcg
     1081 aatttttagt ttctccggcg cgacgccctc gggataaccg aagaacaacg tcgccgtcga
     1141 ttgcgcaaaa tacccggacc ccgccacgac ctccgacgtc tgcatcgatc catccgcgag
     1201 cgtcaacatc gcgcgcgctc ccaccgccgc cgggttcccc gccgccccgc gcaacgccac
     1261 gccaaaactg cgcctcccgc cgcggccgcc attcagcaac gccagcggtc gatcgttgtt
     1321 gcgcgtcacc aacacatccg cccacccgtc ctgattaaaa tcgatcgtcg cgagcgcctt
     1381 cgcgtcgcgg cgcacaa
//
LOCUS       DASPLT010001467         2415 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_182160, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001467 DASPLT010000000
VERSION     DASPLT010001467.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 2415)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2415)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2415
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_182160"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..664
                     /locus_tag="VEA63_15000"
     CDS             <1..664
                     /locus_tag="VEA63_15000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HYD85371.1"
                     /translation="LLVPEPFLAALGYRDDSRLGQTNYIDPVVNSLFVDIGGGTSDLC
                     LVQGYFPGADDQISIPFAGDAIDQLLQDEINRVYPNNGLSRTKVRELKETHGYVGPSR
                     SPVEVKVVIGGKSHTLELGDALARSANALIDKIYPALTTLIQRASSDSVVTLLQNIIV
                     TGGGSQIKGIDTLLQRKLTDDGFESPKVKLAGQDYKRYVALGALKAARAARDNQWQVL
                     LG"
     gene            complement(865..>2415)
                     /locus_tag="VEA63_15005"
     CDS             complement(865..>2415)
                     /locus_tag="VEA63_15005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD85372.1"
                     /translation="AHETFVGSFAEPKDGGNLDTALSSFDGTTVVIVPRAHASEAIGG
                     ATRWRNIHFAVRGMRGKAPVFRLPLMSPGNRRMILSGNAVSFEHLKLVWSYEPNTTKW
                     HTFDTHVRSGAGASTWRVEAHNAAAFTEDVVYVSINERFPVGDFYEWLEAKVFTHALV
                     KPTASETKAGTFMIGFQSGAPASAAFSRAIPDMPLYGFMIKDPAAKPTKLVVLVSGQH
                     PYEGQTKAALQGAIDWILNSKTAEAAAYRAEYVTVVYPFVNPTGELAGLWRGTAFDPS
                     VDTNRHWDTSEKVPARARGFDTVIVHKNAMLKDIAAIGIGEPYAVFDYHQSFGESETE
                     LDYVLHASLSLAADAPAARQVAVTEFAPYFARLNTQAPMAGRPSDLTSQQTLRGLMVA
                     RGVKLPLTFERSVYHTIESERAFGVATVCALVDSVMVAAATEKSEDNVVDSAATASAS
                     SEVVATPLAVSAFGETGRFEGMSLLLGGTNWFSKATVAQSGRDAAAEISDLGMRFASA
                     LPGTASVE"
BASE COUNT          454 a          858 c          663 g          440 t
ORIGIN      
        1 cctgctcgtg ccggagccgt tcctcgcggc gctcggctac cgcgacgatt cgcgtctcgg
       61 tcagacgaac tacatcgatc ccgtcgtgaa ttcgctcttc gtcgacatcg gcggcggcac
      121 cagcgacctc tgtctcgtgc agggatattt cccgggcgct gacgaccaga tcagcattcc
      181 tttcgccggc gacgccatcg atcaactgct gcaagacgag atcaaccgcg tttatcccaa
      241 caacggcctc tcccgcacga aagttcgtga actcaaggaa acgcacggct acgtcggtcc
      301 gagccgttcg cccgtcgaag tgaaagtcgt gatcggagga aaatcgcaca cgctcgagct
      361 cggcgacgcg ctcgcccgct ccgcgaatgc gctcatcgac aaaatctatc ccgcgctcac
      421 gacgctcatt cagcgcgcgt cctccgactc ggtcgtcacg ctgctccaga acatcatcgt
      481 cactggcggc ggttcgcaga tcaaaggcat cgacacgctg ctgcagagga aacttaccga
      541 cgacggcttc gaatcgccga aagtgaaact cgccggccag gactacaaac gttacgtcgc
      601 gctcggcgcc ttgaaagccg cccgcgccgc ccgcgataac cagtggcagg tcctcctcgg
      661 ctgaccggcg ttccgtagcg aagcgcgtaa gcgtttcgtg cctgcgaata tcacccttcg
      721 ccaaactcac gcagtaagaa cgtagagccc gacgttcccg tcgggctctt ttgcgagcga
      781 gccgttatca agccccgacg ggacgtcgcg ctccaccttt tgcgtgcagt tttctcgctg
      841 ccagcctggg tgccagaaag cgcgctactc cacgctggcc gtccctggca gcgcggacgc
      901 aaatctcatc ccgagatcag agatctcggc cgcagcatca cgcccgctct gcgcgacagt
      961 cgctttcgaa aaccaattcg tcccgccgag cagcaaactc attccctcga agcggccggt
     1021 ttcaccgaat gcgctcactg cgagcggcgt cgccacgact tccgatgacg ctgaagccgt
     1081 cgcggctgag tccaccacgt tgtcttccga tttctccgtc gccgcagcca ccatcacaga
     1141 atccactaac gcgcacaccg tcgccacgcc aaacgcccgc tcgctctcga tcgtgtggta
     1201 aacgctgcgc tcgaacgtga gcggcagctt cacgccgcgc gccaccatca agccccgcag
     1261 cgtctgctgc gacgtgaggt cggacgggcg ccccgccatc ggcgcctgcg tgttgagccg
     1321 ggcaaaatac ggcgcgaact ccgtcaccgc cacctgccgc gcggccggag catcggctgc
     1381 gagcgacagc gaagcgtgca acacgtaatc gagctccgtt tcgctctctc cgaaactctg
     1441 gtgataatcg aacaccgcgt aaggctcgcc gatcccgatg gccgcgatgt ctttcaacat
     1501 cgcattcttg tgcacgatca cggtgtcgaa gccgcgcgcg cgcgccggca ctttttcaga
     1561 cgtgtcccag tggcggttcg tgtccacgga cggatcgaac gcggtcccgc gccacagccc
     1621 cgcgagctct ccggtcggat tcacaaacgg gtaaaccacc gtcacatact ccgcgcgata
     1681 cgccgccgcc tccgctgtct tcgaattcaa aatccagtcg atcgcgccct gcagcgctgc
     1741 cttcgtctgc ccttcataag gatgctgccc gctcacgagc acgacgagtt tcgtcggctt
     1801 cgccgcagga tctttgatca tgaaaccgta gagcggcatg tccgggatcg cccggctaaa
     1861 cgccgccgac gccggcgcgc ccgattgaaa cccgatcatg aacgtgcccg ctttcgtttc
     1921 gctcgccgtc ggcttcacca acgcatgcgt gaacacctta gcctcgagcc attcgtagaa
     1981 gtcgccgacg ggaaaccgtt cgttgatcga cacgtaaacc acgtcctcgg tgaaggccgc
     2041 ggcgttgtgc gcctcgaccc gccacgtgct cgcgcctgca cccgagcgca cgtgcgtgtc
     2101 gaacgtgtgc catttggtcg tgttgggctc gtaactccat accagcttga ggtgttcgaa
     2161 cgagactgcg ttgcccgaca aaatcatccg ccgattgccc ggcgacatca gcggcaaacg
     2221 aaacaccggc gctttgcccc gcatcccacg cactgcgaaa tggatattcc gccatcgcgt
     2281 cgcgcctccg atcgcctcgc tggcgtgcgc gcgcggcacg atcaccacgg tcgtgccgtc
     2341 gaaactggaa agcgccgtgt cgagattccc gccgtccttg ggctccgcaa agctgccgac
     2401 aaacgtctca tgcgc
//
LOCUS       DASPLT010001468         1064 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_202388, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001468 DASPLT010000000
VERSION     DASPLT010001468.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1064)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1064)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1064
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_202388"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            128..>1064
                     /locus_tag="VEA63_15010"
     CDS             128..>1064
                     /locus_tag="VEA63_15010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007362887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HYD85373.1"
                     /translation="MPKKTSFNVAVLGAGAIGLDHLGSFKQHPAVRLAAVADTSTGRA
                     RDAAEMFNVPFVSADYREVVSRPEIDIVSIALPNYLHAPVALAALQAGKHVMVDKPMA
                     TNARDAAKLVAEAKKRRVLLMVGQNNRFSPDVQTLKQLVAKGVLGDVYHAKTAWTRRG
                     GIPRIGSWFTQTKFAGGGSTYDIGVHALDRCLYLMGEFDAAAVSGQTYAKFGPRGLGE
                     GTWGKSELDPAKPFDVEDMSVALIKLKSGRTVLLETSWAGHQAVADFNGTQLFGTEAG
                     ALIPNLQLFRKTENGYATESIATSTTLVNPNRMSHF"
BASE COUNT          207 a          376 c          296 g          185 t
ORIGIN      
        1 cgcttcgccg atcaggcgaa gcacgcaacc gtccgcccgg cataaccacc ttcgaagatc
       61 agcgcaaatt agcggttaaa aaatccgttc cctccgcccg ttttcaccct ctcccctctc
      121 tctttttatg cccaagaaaa cttctttcaa cgtcgccgtc ctcggcgccg gcgcgatcgg
      181 actcgatcac ctcggctcgt tcaaacagca cccggcggtt cgtctcgccg ccgttgccga
      241 cacctccacc ggccgcgcgc gcgacgccgc ggagatgttc aacgttccct tcgtctccgc
      301 ggattatcgc gaggtcgtct cccgccccga gatcgacatc gtctcgatcg cgctgccgaa
      361 ttatttgcac gcacccgtcg cactcgccgc gttgcaagcc ggcaaacacg tgatggtcga
      421 caagccgatg gcgaccaacg cccgcgacgc cgcgaagctc gtcgccgagg cgaagaagcg
      481 ccgcgtgctc ctgatggtcg gccagaacaa ccgcttctcg cccgacgtgc agacgttgaa
      541 gcaactcgtc gccaaaggcg tgctcggcga tgtctatcac gcaaaaaccg cgtggacgcg
      601 ccgcggcggc atcccgcgca tcggttcgtg gttcacgcag acgaaattcg ccggcggcgg
      661 cagcacctac gacatcggcg tgcacgcgct cgaccgctgt ttgtatttga tgggcgagtt
      721 cgacgccgca gccgtcagcg gccaaaccta cgcgaagttc ggcccgcgcg gcctcggcga
      781 aggcacgtgg ggcaagagcg aactcgatcc ggcgaaaccc ttcgacgtcg aggacatgtc
      841 ggtcgcgctg atcaaattga agagcggccg caccgtcttg ctcgaaacct cgtgggccgg
      901 ccatcaagcg gtcgcggact tcaacggcac gcaactgttc ggcaccgaag ccggcgcgct
      961 cattccaaat cttcagctct tccgcaaaac ggaaaacggt tatgcgaccg aaagcatcgc
     1021 gacgtccacg acgctcgtga acccgaaccg catgtcgcat ttcg
//
LOCUS       DASPLT010001469         1357 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_263565, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001469 DASPLT010000000
VERSION     DASPLT010001469.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1357)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1357)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1357
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_263565"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..626)
                     /locus_tag="VEA63_15015"
     CDS             complement(<1..626)
                     /locus_tag="VEA63_15015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD85374.1"
                     /translation="MSVLLFHPSVAPFVQQAARAIHEAGQLAAFATSVHDQPQRFPQR
                     AAASLGQLAGRDFAAQFRRRAITEVPLALVQSHPWGELLRIAVGAVDRDGRATDLVWE
                     KTELAFDRRVSRSLHPKLNGVYGFEHSSLSTFQRARELGLRVAYDVPAPDSEYVHALL
                     DREMERFPELRTPYRRHTAVRESRRLARRRAEWKTADLVVAASAFTRD"
     gene            complement(623..>1357)
                     /locus_tag="VEA63_15020"
     CDS             complement(623..>1357)
                     /locus_tag="VEA63_15020"
                     /inference="COORDINATES: protein motif:HMM:TIGR01444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HYD85375.1"
                     /translation="EVIIGGVPVRVPAYFKGGGRADYETAALARLVDWFSFHPEGLLV
                     DVGSSLSLYGLVALSTSVQAEVVAIDSDLVGLKCSRFLCSKAHAPERLDLVHGFAGTI
                     MEPASTLDEILRDTDRVLADPAIPSDVDFIRYHNIPTTQTRRISLDGLWSAVPLLRPL
                     IVKVDVEGAELDVLRGATHLLHRLRPTLLLSVHPQFGVDVAAIREFLALMGYAVDHFA
                     TDHEEHWLCTPLKQTIASKIPPALHS"
BASE COUNT          265 a          370 c          507 g          215 t
ORIGIN      
        1 ctatcgcgcg tgaacgcgga ggcggcgacg acgaggtcgg cggttttcca ttcggcgcga
       61 cggcgggcga ggcggcgcga ctcgcgcacg gccgtgtggc ggcgataggg cgtgcgcagt
      121 tcggggaagc gttccatttc gcgatcgagc agcgcgtgga cgtattcaga atctggagcc
      181 gggacatcgt aagcgacgcg gaggccgagt tcgcgcgcgc gttggaaggt ggacagcgag
      241 ctgtgctcga agccgtaaac gccgttgagt ttggggtgaa gcgaccggct gacacggcga
      301 tcgaaggcga gctcggtttt ttcccagacg agatcggtgg cgcgtccgtc gcggtcgacg
      361 gcgccgacgg caatccgcag gagttcgccc cagggatgag attgcacgag cgcgagcgga
      421 acctcggtga tggcgcggcg gcgaaattgt gcggcgaagt cgcggccggc gagctggccc
      481 agcgacgcgg cggcgcgttg gggaaagcgc tgcggctggt cgtgcaccga ggtggcgaac
      541 gcggcgagct ggccggcctc gtgaatcgca cgcgcggctt gttggacgaa cggtgcgacg
      601 ctgggatgaa acaacagaac gctcacgagt gcagggcggg tgggatcttc gatgcgatgg
      661 tttgcttcag cggtgtgcac aaccagtgct cttcgtgatc ggtggcgaag tgatcgacgg
      721 cgtagcccat gagggcgagg aattcgcgga tggctgccac gtcgacgccg aattgcggat
      781 gaacgctgag aagcaaagtc ggtcgcagac gatgcagcag gtgagttgcg ccgcgcagca
      841 cgtcgagctc ggcgccttcg acgtcgactt tgacgatgag cgggcggagc aacggcaccg
      901 ccgaccacaa cccatcgaga gaaattctcc gggtctgcgt tgtcgggata ttgtggtaac
      961 gaatgaaatc cacgtccgac ggaattgccg gatcggcgag cacgcgatcc gtgtcacgga
     1021 ggatctcatc gagggtggac gcgggctcca tgatcgttcc ggcaaagccg tggacgaggt
     1081 cgaggcgctc cggtgcgtga gctttgctgc agaggaagcg gctgcatttg agtcccacga
     1141 gatcggaatc gatggcgact acctcggctt ggaccgaggt ggacagcgcg accaggccat
     1201 acagtgaaag cgaggaaccg acgtcgacga gcaatccctc ggggtgaaaa gagaaccaat
     1261 cgacgagtcg agcgagggcg gcggtttcgt agtcggcacg accgccgccc ttaaagtaag
     1321 cgggcacgcg aaccggcacg ccgccaatga tcacctc
//
LOCUS       DASPLT010001470         1154 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_160801, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001470 DASPLT010000000
VERSION     DASPLT010001470.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1154)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1154)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1154
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_160801"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..607
                     /locus_tag="VEA63_15025"
     CDS             <1..607
                     /locus_tag="VEA63_15025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HYD85376.1"
                     /translation="ELLEKLDLQRSDRVVLLGDLINRGPDSAGVIELARRHADIALLG
                     NHELRLLNYRKTGDPTHLKKTDYDTLKQLSRRDWAYLEKLPLTYHVRSHDIVLVHGGF
                     LPAQPWRQQPARVVTRIQNIDRDGNPRKRSDAPDCPHWSSFWKGPPFVVYGHTSRPHV
                     HRRKWSLGIDTSCAMGGSLTACILPETKIVQVKAHETYYEG"
     gene            complement(696..>1154)
                     /locus_tag="VEA63_15030"
     CDS             complement(696..>1154)
                     /locus_tag="VEA63_15030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD85377.1"
                     /translation="LPLSQADPVDGLVDAGAVNVRPVSVQTGAAVNHIIADQHSMLFV
                     HAPSEVVLPVANGERRFEGGCAFIEGAYTSGRTDGAEFSIEALTKGGRWEELWRRTLD
                     PLNDLNDRGFQRFAVEVPVGASQVRLRIAPGENNQANWDWTCWGDLRLLP"
BASE COUNT          234 a          414 c          304 g          202 t
ORIGIN      
        1 cgagttactc gagaaacttg accttcaacg ctccgatcgc gtcgtcctcc tgggcgatct
       61 catcaatcgc ggccccgaca gcgccggcgt catcgagctc gcgcgtcgtc acgccgacat
      121 cgcgcttctc ggcaatcacg agcttcgcct gctcaactac cgcaagaccg gcgatcccac
      181 gcacctgaag aaaaccgact acgatacgtt gaaacaactc agccggcgag actgggctta
      241 tctcgagaag ctgccgctca cctatcacgt gcggtcgcac gatatcgtgc tggttcacgg
      301 cgggttcctg cctgcgcagc cctggcgcca gcaacccgcg cgcgtcgtta cgcgtatcca
      361 aaatatcgat cgcgacggga atccgcgcaa acgttccgat gcccccgact gcccccactg
      421 gtcatcgttt tggaaaggcc cgccctttgt ggtctacggc catacctcgc gcccccacgt
      481 gcaccggcgg aaatggtctc tcggcatcga caccagttgc gccatgggtg gcagcctcac
      541 cgcgtgcatc ttgccagaaa cgaagatcgt ccaagtcaaa gctcacgaaa cctactacga
      601 aggctgacga cggatcaccg aggtggcgcc gttgcgccgt cgaaggcacc tcgcctagcg
      661 cgcggatcga cgcgagcatg ctcaaccgcc gcagctcacg gcaaaaggcg caaatccccc
      721 cagcaagtcc agtcccagtt cgcctgattg ttttcacccg gcgcaatacg caggcgcacc
      781 tgtgacgcgc cgaccggcac ctcgactgcg aatcgttgaa atccgcgatc gttcaaatcg
      841 ttcaacggat ccaaggtgcg tcgccacagc tcctcccagc ggccaccttt cgtcagagcc
      901 tcgatgctga attcagcgcc atccgtgcgg cctgacgtat acgctccctc gataaacgcg
      961 cacccgccct cgaaccgccg ctcgccgttt gccaccggca gcaccacctc gctcggagca
     1021 tggacgaaca gcatcgaatg ttgatcggcg atgatatggt tcacggccgc ccctgtttgg
     1081 accgagaccg gacgcacatt caccgcgcct gcatccacga gaccgtcgac cggatcggcc
     1141 tggctcaatg gcaa
//
LOCUS       DASPLT010001471         1005 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_304891, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001471 DASPLT010000000
VERSION     DASPLT010001471.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1005)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1005)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1005
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_304891"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1005)
                     /locus_tag="VEA63_15035"
     CDS             complement(<1..>1005)
                     /locus_tag="VEA63_15035"
                     /inference="COORDINATES: protein motif:HMM:NF012686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD85378.1"
                     /translation="AGFAAWFRQTRVITSMEAVRARMGPANEQVFTWLTLPIGIIVAG
                     IWLYGLAIFCAPVFGIDLQLMILVSGITVVFVAAAGGQWAIVAGDFVQALMLVPITIV
                     AAVWAWVKIGGWSGFEAALPAEHWNVAASDAAGFGKWWLLAIFIEKFFVQNALHGAGR
                     YLNVRDGREAKRAAMLAMLLYGAGSLLWFIPPLAARAMNLDLVAQFPGLTKPSEAAYV
                     AVAAMCLPVGLMGLMVTGIISATLSSMDHGLNRNAGVFVRSVYVPLIRPNAGEREQVL
                     AGRISTLAFGGIVILTALLYSTWKDLGVFNLMFGFSALLGIPYAVPMVLCLFVKRVPD
                     W"
BASE COUNT          219 a          322 c          320 g          144 t
ORIGIN      
        1 ccaatccggc acgcgtttta cgaaaaggca caacaccatg gggacggcgt aggggatgcc
       61 gagcagcgcg ctaaagccga acatcaggtt gaacacgccg aggtccttcc acgtgctata
      121 caacaacgcc gtgaggatca cgatgccgcc aaacgccagc gttgaaatgc ggccggcgag
      181 gacctgttcg cgttcgccgg cgttcggacg tatcagcggc acgtatacgc tgcggacgaa
      241 aacgccggcg ttgcggttga gaccgtggtc catcgacgag agcgtggcgg aaatgatgcc
      301 ggtgaccatg aggcccatca ggccgaccgg caggcacatg gcggcgacgg cgacgtaagc
      361 ggcttcgctc ggcttggtca ggccgggaaa ctgcgcgacg aggtcgaggt tcatcgcgcg
      421 cgcggcgagg ggtggaatga accacagcag cgagccggcg ccgtataaca gcatcgcaag
      481 catcgcggcg cgtttcgctt cgcggccgtc gcggacattc aaatagcggc cggcgccgtg
      541 cagcgcgttc tgaacgaaaa atttttcgat gaagatcgcg agcagccacc atttgccaaa
      601 acccgccgca tcggacgcgg cgacgttcca atgctccgca ggcagtgccg cttcgaaccc
      661 ggaccagccg ccgattttca cccaggccca caccgcggcg acgatcgtga tgggaaccag
      721 catcaatgcc tggacgaaat cgcccgcgac gattgcccat tgaccgccgg ccgcggcgac
      781 gaacacgacc gtgatgccgc tgaccaggat catgagttga agatcgatgc cgaacaccgg
      841 cgcgcaaaag atcgcgagcc cgtagagcca gatcccggcg acgatgatgc cgatcggcag
      901 cgtgagccac gtgaagacct gctcgttggc cggacccatt ctggcgcgca ccgcctccat
      961 cgacgtgatc acccgcgtct ggcgaaacca ggcggcaaac cccgc
//
LOCUS       DASPLT010001472         1455 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_160822, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001472 DASPLT010000000
VERSION     DASPLT010001472.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1455)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1455)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1455
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_160822"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..832)
                     /locus_tag="VEA63_15040"
     CDS             complement(<1..832)
                     /locus_tag="VEA63_15040"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HYD85379.1"
                     /translation="MRNGIIAGGNWIVDHIKVLDRWPAQDALASILRQSTGSGGSPYN
                     ILKDLARLGASVPLAGIGLVGNDDDGRAIIRDCQAHGIDTTRLSVTTAAATSYTDVMT
                     VESSGRRTFFHQRGANAYLGEEHFDFSTTEAKIFHLGYVLLLDRLDQLVNGRPRVCDV
                     LQKARDARMLTSLDCVSEDSERFQTIVAPVLPVVDVLFANDFEAEKITGVPLRSEGRI
                     QSKAVDVAARDLLRRGVKAWVIIHFPEAVYACGASGEAIWQPSLRIPTETIAGAAGAG
                     D"
     gene            complement(836..>1455)
                     /locus_tag="VEA63_15045"
     CDS             complement(836..>1455)
                     /locus_tag="VEA63_15045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD85380.1"
                     /translation="KPINWKRQNKFCGEPGVMNDLGLHVAHVPLRLGWKPQRLHAQLQ
                     KIYTERPDGKGGMASCDTWDNATLHCLAPVAGQTAPLTLEMKRMAPTETNTWEIEILG
                     TERGVRYSTKEPKTLWQFRRDKEQWWEKTDLGFGTPFKTITGGIFEPGFPDILQQMWA
                     AFLAERAGQLNGRFGCATPDEAVASHEIWAAALASHRQQQAVTLS"
BASE COUNT          241 a          497 c          439 g          278 t
ORIGIN      
        1 catcgcccgc acccgccgct cccgcgatcg tctcggttgg aatccgcagg ctcggctgcc
       61 agatcgcttc gcccgatgcg ccgcacgcat acacggcctc gggaaaatgg atgatcaccc
      121 acgccttcac cccgcgccgg agaagatccc gcgccgcaac gtcgacggcc ttcgattgaa
      181 tccgcccttc gcttcgcagc ggaacaccgg tgattttctc cgcctcgaaa tcgttggcga
      241 agagcacgtc gaccacgggc agcaccggcg ccacgatcgt ctgaaatcgt tcgctgtctt
      301 cgctgacgca atcgagcgac gtgagcatcc gcgcgtcgcg cgccttctgc agcacatcgc
      361 acacgcgcgg acggccgttg accaattgat ccagccggtc gagcagcagc acgtaaccga
      421 ggtgaaagat tttcgcctcg gtcgtgctga aatcgaagtg ctcctcgccg agatatgcgt
      481 tcgcgccgcg ctgatggaaa aaagtccgcc ggccactgct ttcgaccgtc atcacgtccg
      541 tgtaactcgt cgcggctgcc gtcgtgacgg acagtcgtgt cgtgtcgatg ccgtgcgcct
      601 ggcaatcgcg aataatcgcg cggccgtcgt cgtcgttgcc caccagaccg attccggcca
      661 gcggcaccga cgcaccgagc cgcgcaagat cttttaggat gttgtagggc gaaccgccgc
      721 tgccggtaga ctgacgcaag atcgacgcga gcgcgtcctg cgccggccag cgatcgagaa
      781 ccttgatgtg atcgacgatc cagttgccgc cggcgatgat tccgttgcgc atggttcagg
      841 agagggtcac ggcctgctgc tggcggtgcg acgcgagcgc cgcggcccag atttcgtgcg
      901 aagccacggc ttcgtccggc gtcgcgcaac cgaagcgccc gttgagctgc cccgcccgct
      961 cggccagaaa cgccgcccac atctgctgca ggatgtcggg aaaacccggt tcgaagatcc
     1021 cgccggtgat cgtcttgaac ggggtgccga aaccgagatc ggtcttttcc caccattgct
     1081 ccttgtcgcg gcgaaactgc cacagcgtct tcggctcctt cgtcgagtaa cgcacgccgc
     1141 gctctgtgcc gagaatctcg atctcccatg tattcgtttc cgtcggcgcc atccgcttca
     1201 tctcgagcgt gagcggcgcg gtttgtccgg cgaccggcgc gaggcagtgc agcgtggcgt
     1261 tgtcccacgt gtcgcacgac gccatgccac cctttccgtc gggtcgctcc gtgtagattt
     1321 tctgcagctg tgcgtgcagg cgttgcggct tccagccgag tcgcaacggc acgtgcgcga
     1381 cgtgcagccc gaggtcgttc atcacgccgg gctcgccgca aaacttgttc tgccgcttcc
     1441 aattgatcgg cttcg
//
LOCUS       DASPLT010001473         2644 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_18125, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001473 DASPLT010000000
VERSION     DASPLT010001473.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 2644)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2644)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2644
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_18125"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..615)
                     /locus_tag="VEA63_15050"
     CDS             complement(<1..615)
                     /locus_tag="VEA63_15050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HYD85381.1"
                     /translation="MKIRKALVTAANPRQRTLPLQTLIDSQGESKTALQIVLEEAAGA
                     GIEEFCVIVCPGDEAAYAEASGPLRSRVRFVPQTAARGYGHAILLGRDFIGEEAFLHL
                     VGDHLHVANGQTSCARQLIEIAAAENASVSAVQPTRESQLTSYGTIGGRLVGSDKPLY
                     QIEAVLEKPTPSVAEQHLIVPGLRAGFYLCFFGLHVLGAEIFSLL"
     gene            915..1394
                     /locus_tag="VEA63_15055"
     CDS             915..1394
                     /locus_tag="VEA63_15055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HYD85382.1"
                     /translation="MPAKSTRRKKAATRVPRVLRGTVFITRQVHFNAAHRLHNPSKSQ
                     AWNTRQYGLCTNPHWHGHNYVLEVTVKGQPDPETGYIMDLAELKRILHVAVVDKCDHH
                     NLNDEVAFLRGVIPSTENLVIAFWEQIEPLLPAGKLHCVRLFETPRNFAEYYGPEVA"
     gene            1574..2554
                     /gene="folE2"
                     /locus_tag="VEA63_15060"
     CDS             1574..2554
                     /gene="folE2"
                     /locus_tag="VEA63_15060"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003933 - GTP cyclohydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0035998 - 7,8-dihydroneopterin
                     3'-triphosphate biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase FolE2"
                     /protein_id="HYD85383.1"
                     /translation="MSKKKPMYLHRSASGAAPRIARAYDPKFTVTDAYRATLPDMMEA
                     AHDAIQGAHVPIQQVGVSNFKLPLKFRTKEGGVLTLEASITGTVSLEADLKGINMSRI
                     VRSFYDHKDDVFTGEWLGKILCAYLKNVKSKDARLKVSFSYPMLHRSLRSGLEGYQFY
                     DVAFEGVLARDGEYRRFIHFDFVYSSACPCSAELSEHARDTRGVYSVPHSQRSKARVV
                     IEEAPKKKIWIEDVQALCLRALQTETQVMVKREDEQAFAELNGAHLKFVEDAARLLYR
                     EFDAEKRIKDFRIACSHLESLHSHDAVSVICKGVKGGFAADFMDFGSLVC"
BASE COUNT          530 a          658 c          951 g          505 t
ORIGIN      
        1 gaggagcgaa aagatttcgg cgccgaggac gtgaagaccg aagaagcaga gatagaaacc
       61 agcgcgaaga ccggggacga tgaggtgttg ttcggcgacg gagggcgtgg gtttttcgag
      121 aacggcctcg atctggtaga gcggtttatc gctgccgacg aggcggccgc cgatggtgcc
      181 gtaggacgtg agctggctct cgcgcgtggg ttgcacggcg gagacggagg cgttttccgc
      241 ggcggcgatt tcgatgagct ggcgggcgca actggtttgt ccgttggcga cgtgaaggtg
      301 atcgccgaca agatgcagga aggcttcttc accgatgaag tcgcggccga ggaggatggc
      361 gtggccgtag ccgcgcgcgg cagtttgagg cacgaagcgc acgcgggaac ggagagggcc
      421 agaggcttcg gcgtaggcgg cttcgtcgcc ggggcacaca atgacgcaga actcctcgat
      481 gccggcgccg gcggcttctt cgaggacgat ttgcaacgcg gttttcgatt cgccttggga
      541 atcgatgagc gtttggagcg ggagcgtgcg ctggcgcgga ttggcggcgg tgacgagggc
      601 tttgcgaatt ttcatgcggc ggagctggag cgatttccgg ctagcagtca ggcaaggggg
      661 tggggtggtt caactggaaa gcggggatcg gggaaacgcc ggaggaggtg gtgaggggtt
      721 gtttgaggac tacgattttg aaatcgggga atgtgaaatc gagcagcttg aaatcgggat
      781 cttgaaattg ggttgaaagt gggcagcggg gtgttgggga ggggaggcgt ttgtcggggc
      841 gtaaagccct cccgccgttt cgttacggtg gcggcgattg accgcgcggg gaatgtcgct
      901 acttctgacc ctccatgcca gcgaagtcca ctcgccgaaa gaaagccgcc actcgcgttc
      961 cgcgcgtgtt gcgaggaacg gtgttcatca cgcgccaggt gcatttcaac gccgcgcacc
     1021 ggttgcacaa tccgtcgaag agccaggcgt ggaacacgcg ccaatacggg ctttgcacga
     1081 atccgcactg gcacggtcac aattacgtgc tggaggtcac ggtgaagggg cagcccgatc
     1141 cggagacggg ttacatcatg gatttggcgg agttgaagcg gatcctgcac gtggcggtgg
     1201 tcgacaagtg cgatcaccac aacctgaacg acgaggtggc gtttttgcgc ggggtgatcc
     1261 cctcgacgga gaatctggtg atcgcgtttt gggagcagat cgagccgctg ttgccggcgg
     1321 ggaagctgca ttgcgtgcgg ctgtttgaaa cgccgcgcaa cttcgcggag tattacgggc
     1381 cggaggttgc ttaaccacgg atggacacga attaggagtc ccgtcgggca ttcgctttct
     1441 tcattcgtgt gcattcggtt ccctgcgcgg gttctttcac ttggacaaga aacggaaaag
     1501 gcgggctgaa gcccgcccta cgtgaggagc ggttatcgtc acccgcatct cgcggaatca
     1561 ggaaaaaacg aacatgagca aaaagaaacc aatgtatttg caccgctccg cgagcggggc
     1621 ggcgccgcgt atcgcgcgcg cttacgatcc caaattcacg gtaaccgacg cgtatcgcgc
     1681 gacgctgccg gacatgatgg aggcggcgca cgacgcgatc cagggcgcgc atgtgccgat
     1741 ccagcaggtg ggcgtatcca acttcaaatt accgctgaag tttcgcacga aggaaggcgg
     1801 ggtcctgacg ctcgaagcga gcatcaccgg cacggtgtcg ctcgaggcgg atttgaaagg
     1861 catcaacatg agccggatcg tgcggtcgtt ctacgatcac aaggacgacg tgttcaccgg
     1921 cgaatggctg gggaagattc tctgcgcgta tctgaagaac gtgaagagca aggatgcgcg
     1981 gttgaaggtg agtttttcgt atccgatgct gcaccgcagc ctgcgcagcg ggctggaagg
     2041 ttatcagttc tacgacgtgg cgttcgaggg cgtgctggcg cgcgatggcg agtatcggcg
     2101 gttcattcat ttcgacttcg tgtattcgtc ggcgtgcccg tgctcggcgg aattgtcgga
     2161 gcacgcacgc gacacgcggg gcgtttacag cgtgccgcat tcgcagcgga gcaaggcgcg
     2221 cgtggtgatc gaggaagcgc cgaagaagaa gatctggatc gaggatgtgc aggcgctgtg
     2281 cttgagggcg ctgcagaccg agacgcaggt gatggtgaag cgcgaggatg agcaggcgtt
     2341 tgcggagttg aacggcgcgc acctgaagtt cgtcgaagat gcggcgcggc tgctttatcg
     2401 ggagttcgac gcggagaaac ggatcaagga tttccgcatc gcgtgctcgc atctcgaatc
     2461 gctgcattcg catgacgcgg tgagcgtgat ctgcaagggc gtgaagggtg ggtttgcggc
     2521 ggacttcatg gatttcggat cgctcgtctg ctagccgccg cgggacgcgg cggaagctcc
     2581 aaggaccaag ctccaagctc cagggaagct tcaaatttca aaccttaata cgacccgaga
     2641 tgcc
//
LOCUS       DASPLT010001474         1649 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_119366, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001474 DASPLT010000000
VERSION     DASPLT010001474.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1649)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1649)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1649
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_119366"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            72..626
                     /locus_tag="VEA63_15065"
     CDS             72..626
                     /locus_tag="VEA63_15065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HYD85384.1"
                     /translation="MRERRQAKKESLAGSLLLAHPALKDPNFRRTVILMSAHSPEGAM
                     GVVLNRPTGKHLGELNGDFALGTLASVPIFSGGPVQTEQLLLVAWQPHEEGFRLHFGV
                     EPDRATQLATEEGTQLRAFLGYSGWSGGQLENELKASTWIVTDVPAELLDHAPGKDLW
                     RRVLGALGEEWRLLAEEPEDTSLN"
     gene            711..836
                     /gene="ykgO"
                     /locus_tag="VEA63_15070"
     CDS             711..836
                     /gene="ykgO"
                     /locus_tag="VEA63_15070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L36"
                     /protein_id="HYD85385.1"
                     /translation="MKVVSSIKSAKKRHPACQVVRRKGKIYVINKVEPRYKARQG"
     gene            857..1111
                     /locus_tag="VEA63_15075"
     CDS             857..1111
                     /locus_tag="VEA63_15075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007360010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HYD85386.1"
                     /translation="MKAEGHPSLNNVCFLDIGTGRRFLTKSTMKSARKESIDGVEYFI
                     VARDVTADSHPAYTGEKRLVDTAGRVEKFTSKFKRSAAKK"
     gene            1343..>1649
                     /locus_tag="VEA63_15080"
     CDS             1343..>1649
                     /locus_tag="VEA63_15080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007362726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 family protein"
                     /protein_id="HYD85387.1"
                     /translation="MRIRAFQGLVPQPALAPEVACVPYDVVNTKEAAALAAGKPHSLL
                     HVDRAEIDLPADTDPYSEIVYTTARQNFQKLQRDGVLGRESGPCLYVYQQQMGEHRQR
                     "
BASE COUNT          320 a          441 c          567 g          321 t
ORIGIN      
        1 ggggatcaag attttgcagc tgtaattttg acggaagcga aacggcgcgt tagggataac
       61 gcgtctcact catgcgcgaa cgacgtcagg cgaaaaagga gagtcttgcg ggctcgttgc
      121 tgctggcgca tcccgcgttg aaggacccga atttccggcg gacggtgatc ctgatgtcgg
      181 cgcacagtcc ggaaggggcg atgggcgtgg tgttgaaccg gccgacgggg aagcacctgg
      241 gcgagttgaa cggggatttt gcgctgggca cactggcgag cgtgccgatt ttttcgggcg
      301 ggccggtgca aacggagcag ctcttgttgg tggcgtggca gccgcacgaa gagggctttc
      361 ggcttcattt cggagtggaa cccgaccggg cgacgcaact ggcgacggag gaagggacgc
      421 aactgcgggc gtttctcggg tattcggggt ggtcgggcgg gcagttggag aacgaattga
      481 aggcgagcac ctggatcgtg acggatgtgc cggcggaatt gctcgatcat gcgccgggca
      541 aggatttgtg gcggcgggtg ttgggggcgt tgggcgagga gtggcggctg ctggcggagg
      601 agccggagga tacgagtttg aattgaaagc ggcgaggcgg ggtcgggaga ccccgcccta
      661 caaaacggag gttgacaggg gtggggggcg tctctgtttt cggctctttt atgaaagtcg
      721 tttcctccat taaatcggcc aagaagcgtc acccggcctg ccaggtcgtg cgtcgcaagg
      781 gcaagatcta cgtcatcaac aaagtcgagc cgcgttacaa agcgcggcag ggctgattca
      841 actttttccc cgcgccatga aagccgaagg ccatccctct ctcaacaacg tctgcttcct
      901 cgatatcgga accggccgtc gttttctcac gaagtccacg atgaagtcgg cccgcaagga
      961 gtcgatcgac ggtgtggaat acttcatcgt cgcgcgtgac gtgaccgccg attcgcaccc
     1021 cgcctacacg ggcgagaagc gcctcgtgga cacggccggc cgcgtcgaga agttcacgtc
     1081 gaagttcaag cgcagcgccg cgaagaagta agcgcgagcg cgacgacgtc gcgaagaagc
     1141 gaaccaagat ttgcgaaagc cctccggaaa cggagggctt tttttgtagg cgcgcgccgc
     1201 cctccgtccg cggctgccat ctgcaccaaa ctatgacgca attgcgtcac agtttggcgc
     1261 gagggtggaa ggcggccggt gtttttttcc gtgtgtttgg cgtggtcggc gggcaattct
     1321 cagcattctc atatttctca ccatgcgtat ccgtgctttc caaggtctcg ttccgcaacc
     1381 tgcgctcgcg cctgaagtgg cgtgtgtgcc ttacgacgtg gtcaacacga aggaggcggc
     1441 cgctctcgcc gcgggaaaac cgcacagcct gctgcacgtc gatcgcgcgg agatcgatct
     1501 gccggcggac accgatccgt attcagaaat cgtctacacg acggcgcggc agaattttca
     1561 gaaactgcag cgtgatggcg tgctcgggcg cgagagcggg ccgtgccttt acgtttatca
     1621 gcaacagatg ggcgagcatc gtcagcgcg
//
LOCUS       DASPLT010001475         1930 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_241955, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001475 DASPLT010000000
VERSION     DASPLT010001475.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1930)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1930)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1930
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_241955"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(422..1126)
                     /locus_tag="VEA63_15085"
     CDS             complement(422..1126)
                     /locus_tag="VEA63_15085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HYD85388.1"
                     /translation="MSDSLIKLEGVTKVFLTDEVETHALSGIHMDILKGEYVSIAGPS
                     GCGKSTLLSILGLLDSPSDGSYILNGRPVQGLSLPERARIRNREIGFIFQSFNLIGDL
                     TVYENVELPLTYRGMPSAERKVAAMEALEKVGMGHRAKHLPSQLSGGQQQRVAVARAL
                     AGKPAVLLADEPTGNLDSKNGDSVMQLLAELHKGGATIVMVTHDARFARNADRTIHIF
                     DGRVVQEQVESDPTRA"
     gene            complement(1338..>1930)
                     /locus_tag="VEA63_15090"
     CDS             complement(1338..>1930)
                     /locus_tag="VEA63_15090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HYD85389.1"
                     /translation="QAKLRQDELDALTVRAGMHGILQILPVEVGAQVQPGSNLARVAD
                     PLRLKAEIRIAETQARDIEIGQLASVDTRNGVVAGKVGRIDPSVQNGTVTVDVLFTEP
                     LPRGARPDLSVDGTIELERLDDVVYVGRPAFGQERSTVGIFKLDPAGVYATRTQVQLG
                     RSSVNTIEVVSGLQPGDRVILSDMSPWDSNDRVKLN"
BASE COUNT          343 a          461 c          758 g          368 t
ORIGIN      
        1 atcttagatg gccgatgagc gatttatgat ttcaagattc tggtcatcga agggcggcgg
       61 gacggagcgg gtggaggagt tttgcgcgtt gccgctggcg ggcggtgagt cggggcgtaa
      121 agcccctccc acagttgtgt ccactgcggc gggcgggagg aaaacacgcg gagggctgcg
      181 gaagttgcgg ccaaaaaaaa tgccgcgata aagcggcgtg gttgccggcg ggcccggacc
      241 ctaaatcgag cccgccggct cagacgcgcc cgtgcatgca cagacgggca caagtgagaa
      301 atgaagaaag acgtcgagat tgggtggtct tgacgatagg atcctggttg ccggcgatgg
      361 cgtgcgttgt tcgccggtga cgcgctatcg ccggcgagca ggctcctact ccggggagga
      421 atcaggcgcg agtcgggtcg gactcgactt gttcctggac gacgcggccg tcgaagatgt
      481 ggattgtgcg atcggcgttg cgggcgaaac gagcgtcgtg cgtcaccatg acgatcgtgg
      541 cgccgccttt gtggagctcg gcgaggagct gcatgacgga gtcgccgttt ttcgaatcga
      601 ggttgccggt cggttcgtcg gcgagaagga cggcgggttt gccggcgagc gcgcgggcga
      661 cggcgacgcg ctgttgttga ccgccggaga gctgggaggg gagatgtttc gcgcggtggc
      721 ccatgccgac tttttcgagg gcctccatcg cggcgacttt gcgctcggcg ctgggcatgc
      781 cgcgataggt gagcgggagc tcgacgtttt catagacggt gagatcgccg atgagattga
      841 acgattggaa aatgaaaccg atctcgcgat tgcggatgcg cgcgcgctcg gggagcgaga
      901 ggccttggac ggggcggccg ttgagaatgt aggagccgtc ggagggcgaa tcgagcaggc
      961 cgaggatgga gaggagcgtg gatttgccgc agccggacgg gccggcgatg gaaacgtatt
     1021 cgcccttcag gatatccatg tggatgccgg agagcgcgtg ggtttctact tcgtcggtga
     1081 ggaagacttt ggtgacacct tcgagtttaa tcagtgagtc ggacatggta ttttcggagt
     1141 tgggggttgg ggcggagatt gaagtttgga gttcggagtt cggagatcgg gtttagcgcc
     1201 gacggctgga gcgaacgcgg aggtgaggac tcgctcgaaa cactgcagat gggcggaagg
     1261 ttacaggaag tttgttgaag ggtggatttt gtggcgggag tcggggcgta aagcccctgc
     1321 cacagtcttt aggtggatca gttgagtttc acgcggtcgt tggaatccca cggggacatg
     1381 tcggagagga tcacgcggtc gccgggttgg aggccgctga cgacctcgat ggtgttgacc
     1441 gagctgcgac cgagttggac ttgggtgcgc gtcgcgtaaa cgcccgcggg gtcgagcttg
     1501 aagatgccaa cggtgctgcg ttcctgcccg aaggcggggc ggccgacgta gacgacgtcg
     1561 tcgagccgct cgagttcgat cgtgccgtcg acggagagat cggggcgggc gccgcgcgga
     1621 agcggttcgg tgaagagaac gtcgacggtg acggtgccgt tttgaacgga gggatcgatg
     1681 cggccgacct tgccggcgac gacgccgttg cgcgtgtcga cggaggcgag ttgaccgatc
     1741 tcgatgtcgc gcgcctgagt ttcggcgatg cggatttcgg ccttgaggcg aagcggatcg
     1801 gcgacgcggg cgagattgga accgggctgg acctgggcgc cgacctcgac gggaagaatt
     1861 tgaagaatac cgtgcatgcc ggcgcggacg gtgagggcgt cgagttcgtc ctggcggagt
     1921 ttcgcttgag
//
LOCUS       DASPLT010001476         1758 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_182318, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001476 DASPLT010000000
VERSION     DASPLT010001476.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1758)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1758)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1758
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_182318"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            137..>1758
                     /locus_tag="VEA63_15095"
     CDS             137..>1758
                     /locus_tag="VEA63_15095"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HYD85390.1"
                     /translation="MAEHEIPGLAAVLVQNGETVFSAGYGMADVRRQIPVDAQRTIFP
                     LGSVSKLLTTVAVLQLVENGTLDLNADIGPYLAPLRYDQRFTAPVTLRHLLTHTDGFD
                     VRWLVGGAARVPEKVQPLSALMADLPPRIMPPGETYLYSDVGMTIAGYVVERVSHRPF
                     ADYMEQHVFEPLGMQQSSFRPTRENYARDRATGYAYNQEGRLAPVPVMYPHALPASGA
                     TAPISDLAPFMISLLPSRDSGANLLLNAATVREMWRQQFAHHAAMPGTAFGFYEFFHH
                     GHRALVHGGLLPGFTTVMVLLPEAGVGLCVAGNRFDLIGAFEDQLLRQLIEHFLPIPA
                     EVSPSTFVARPQPRDATLLEGTYRCDQYSRLSIDKLFVFAGMATELRVTANADGSLRI
                     GPREGRWIEIADRLYANETTGERLAFRDDPLTGDIRIVGSAQFMSYHRIGVLERLGAH
                     YALFAGLPLLCLSATCVGGWSAWRSRRQGLSLRSGWLVARRASMTVTLIALLIFLGWF
                     AASLAQLDFFTAAVGEPRSLAAIPYAGFVLALV"
BASE COUNT          261 a          626 c          514 g          357 t
ORIGIN      
        1 cttcgcgcgg cacctgcgaa ccgcgtgcgt tgccggcttc gcctgcgccg cgctcgccag
       61 tcccgccacg gcaaccggcc cgactgaacc ggccgcggct caccgcgtat ttttcgacga
      121 cgtcttctcc ggcctgatgg cggaacacga aatacccggc ctggccgccg tgctggtgca
      181 aaacggtgaa accgttttca gcgcaggtta cggcatggcg gatgttcggc ggcagattcc
      241 ggtcgatgct caacgcacca tttttccgct cggctcggtg tcgaagctgc tcaccaccgt
      301 cgccgttctc caactcgtcg agaacggcac gctcgatctc aacgccgaca tcggccctta
      361 cctcgcgccg cttcgctacg accagcgctt cacggcaccg gtgaccttgc gccatttgct
      421 gacgcacacg gatggtttcg acgtgcgctg gctcgtcggc ggcgcggcac gcgtgcccga
      481 gaaagttcaa ccgctctccg cgctgatggc agatcttccg ccgcgcatca tgccgccggg
      541 agaaacctac ctttacagcg acgtcggcat gacgatcgcc ggctacgtcg tggagcgcgt
      601 ttcgcaccgg ccgtttgccg actacatgga gcagcacgtg ttcgagccgc tcggcatgca
      661 gcaatcttcg tttcgtccaa cgcgagaaaa ctacgcgcgc gatcgcgcca ccggttacgc
      721 ttacaaccag gaaggccgtc tcgctccggt gcccgtgatg tatcctcacg cactgccggc
      781 gtcgggcgcc acggcaccga tctccgacct cgcgccattc atgatttcgc tgcttccttc
      841 gcgcgactcc ggcgcgaatc tcctgctgaa cgccgccaca gtccgtgaaa tgtggcgcca
      901 gcaattcgcc catcacgccg ccatgcccgg cactgccttc gggttctacg aattttttca
      961 ccacggtcat cgcgcgctcg tgcacggcgg actgctgccg ggcttcacga cggtgatggt
     1021 gctgcttccc gaggccggcg tcgggctttg cgtcgcgggc aaccgtttcg atctgatcgg
     1081 cgccttcgag gaccaactgc tgcggcagtt gatcgagcac ttccttccca tcccggcgga
     1141 agtttcgccg tcgacgtttg tcgctcgccc ccaaccgcgc gacgccacct tgcttgaagg
     1201 aacgtatcgc tgcgaccaat actcgcgcct gtcgatcgac aagctcttcg tctttgccgg
     1261 catggccacc gaactgcgcg tcacggcgaa cgcggacggc agcctgcgaa tcgggccgcg
     1321 cgagggtcgc tggatcgaga tcgccgaccg gctctacgcg aacgaaacca ccggcgaacg
     1381 cctggctttc cgcgacgatc cgctcaccgg tgacatacga atcgtcggtt cagcccagtt
     1441 catgtcgtat caccgcatcg gtgttctcga gcgactcggc gcgcactacg cgctcttcgc
     1501 gggcttgccg ctgctttgcc tctcggcgac atgcgtcggt ggatggtcgg cctggcgctc
     1561 gcggcgacag ggtctgagtc ttcgctccgg ctggttggtc gcccgacggg cctcgatgac
     1621 cgtgacgctc atcgccctgc ttattttcct cggctggttt gccgcatcgc tcgcgcaact
     1681 cgacttcttc accgctgcgg ttggcgaacc gcgttcgctc gccgcgattc cttacgccgg
     1741 cttcgtgctc gcactcgt
//
LOCUS       DASPLT010001477         2244 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_57197, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001477 DASPLT010000000
VERSION     DASPLT010001477.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 2244)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2244)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2244
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_57197"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..625
                     /locus_tag="VEA63_15100"
     CDS             <1..625
                     /locus_tag="VEA63_15100"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HYD85391.1"
                     /translation="APDFRGSVVTIPYGAPQIDLPNVPARPARARGDKLRALFVGSLG
                     QRKGLSYLFRAIRSLGDSVELTVIGSRPLVECPALDRELRAVRWIPTCTHHEVLAEMA
                     AHDVFVFPSLFEGFGLVLLEAMAMGLPIVTTPHTAGPDLITDGIEGFIVPVRSSETIA
                     ERLDLLRRNPDLRVDMAERARARASEFTWAQYGESLAACVSSGLVHA"
     gene            629..1984
                     /locus_tag="VEA63_15105"
     CDS             629..1984
                     /locus_tag="VEA63_15105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD85392.1"
                     /translation="MEKLLKTLIWLYIVLLIFEGSLRKWVLPSFSDPLLIVRDPVVMA
                     IYLLAFLTGRFPFNGFIAATAVLAVCSAAASLFVGNTNPLVVAYGIRINYGHLPLIWV
                     MGQTLTRKDVERIGCFLLLVAIPMTLLMVMQFRSPMDAPINRGIGGDEGGQIFGAMGR
                     IRPPGFFSFITGPQAFFPLVAAFFLHQASGSRRLWWPVLIACGLAILISLPVSISRTA
                     MLATGIVAMVFVLTMPRAGIGFGSKFSTAITLGAVAIAVSFLPIFGEAREVFMSRWET
                     ASIGNDGDAWGSIFGRILGGFTQPFHWASVAPFFGHGIGVGSNVGARLLSGTVGFLLA
                     EDEWGKIFLELGPVLGGAFILLRISIAVNLGWKALGALFQHRDNLPILLVSATAIPIM
                     MNQWAPPTLLGFAVVGGGLILGSLNYETDEDDIEEVANEETDNDVAEAEPVRMPIRER
                     Y"
     gene            2018..>2244
                     /locus_tag="VEA63_15110"
     CDS             2018..>2244
                     /locus_tag="VEA63_15110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HYD85393.1"
                     /translation="MRVLLIGNFAPDRQESMLRFANMLTAGLVRHGHEVRTWAPEPRL
                     VRLLPHYRYGGPAKLVGYVDKFLLFPRHVRHR"
BASE COUNT          324 a          762 c          654 g          504 t
ORIGIN      
        1 tgccccggat tttcgaggct cggtcgtcac gattccctat ggcgcgccgc agattgattt
       61 gccgaacgtt cccgcgcgcc ccgcgcgcgc acgcggcgac aagctccgcg cgctcttcgt
      121 cggctcgctc ggtcaacgca agggcttgag ctacctgttt cgcgccattc gttcgctggg
      181 cgacagcgtt gaactcacgg tcatcggcag ccggccgctc gtcgaatgcc ccgcgctcga
      241 tcgcgaactc cgcgcggtgc gttggattcc aacctgcacg catcacgaag tcctcgcgga
      301 gatggcggct cacgacgtat tcgtttttcc atcactgttc gaaggcttcg gtctcgtgtt
      361 gctcgaagcg atggcgatgg gtctgccgat cgtcaccaca ccgcacaccg ccggccccga
      421 tctcatcact gacggcatcg aaggtttcat cgtgccggtg cgctccagcg aaacgatcgc
      481 cgagcggctc gacttgcttc gccgcaatcc cgatctccgc gtcgacatgg cggagcgcgc
      541 ccgcgcccgc gcgagcgaat ttacctgggc gcaatacggc gaatcgctcg ccgcctgcgt
      601 ctcctccggc ctcgtccacg cctgatccat ggaaaagctg ctcaagacgc tcatctggct
      661 ctacatcgtg cttctcattt tcgagggctc gctgcgaaaa tgggtgctgc cctcgttctc
      721 cgatccgctg ctgatcgttc gcgatccggt tgtgatggcg atttacctgc tcgcgttttt
      781 gaccggtcgc tttccgttca acggtttcat cgctgcgacc gccgtgctcg cggtatgctc
      841 cgcggccgcc agtctgttcg tcggtaatac caacccgctc gtggtcgcct acggcatccg
      901 gatcaactac ggccatctgc cgctcatctg ggtcatgggt cagacgctca cgcgcaagga
      961 cgtcgaacgc atcgggtgtt ttctcctgct cgtcgcgatt ccgatgacgc tgctgatggt
     1021 catgcaattc cgctcgccga tggacgcccc gatcaaccgc ggcatcggcg gtgacgaagg
     1081 cggccagatt ttcggcgcga tgggccgcat tcgtccgccg ggcttctttt ctttcatcac
     1141 cggtccgcag gcgttcttcc ccttggtggc ggcgttcttc ctccatcagg cgagcggcag
     1201 ccgtcgcttg tggtggcccg tgttgatcgc ctgcggtctc gcgattctaa tttcgttgcc
     1261 cgtttcgatc agccgcaccg cgatgctcgc gaccggcatc gtcgcgatgg tatttgttct
     1321 caccatgccg cgcgccggca tcggcttcgg ctcgaagttc agcacggcga tcacgctcgg
     1381 tgctgtcgcc atcgctgtat cctttttgcc gatattcggc gaggcgcgcg aggtgttcat
     1441 gtcccgctgg gagaccgcct cgatcggcaa cgatggcgat gcgtggggca gcattttcgg
     1501 ccgcatcctc ggcggtttca cgcagccgtt tcactgggcc tccgtcgcgc cgtttttcgg
     1561 ccacggtatt ggcgtcggtt cgaacgtcgg tgcacgcctg ctctccggca ccgtcggttt
     1621 ccttctcgcg gaggacgaat ggggcaaaat cttcctcgaa ctcggcccgg tgctcggcgg
     1681 cgcgtttatc ctgctgcgca tttcgatcgc ggtgaatctc ggctggaaag ccctcggcgc
     1741 gctctttcag caccgtgaca atcttcccat cctcctcgtg tcggcgaccg cgattccgat
     1801 catgatgaat caatgggcgc cgcccaccct gctcggcttc gccgtcgtcg gcggcgggct
     1861 cattctcggt tcgctcaact acgaaaccga cgaggacgac atcgaagaag tcgccaacga
     1921 ggaaacggac aacgacgtcg ccgaggccga gccggtgcgc atgcccattc gcgaacgcta
     1981 ctgaaccgcc gcatttccac cgctcactct ttttcccatg cgcgttttgt tgatcggtaa
     2041 tttcgcccct gaccgccagg aaagtatgct gcgtttcgcg aacatgctga ccgccggcct
     2101 cgtgcggcat gggcatgagg tgcgcacgtg ggcgcccgag ccgcgactcg tccggctgct
     2161 cccgcactat cgttacggcg gccccgcgaa actcgttggc tacgtcgaca agttcctgct
     2221 tttcccgcga cacgtccgtc atcg
//
LOCUS       DASPLT010001478         1081 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_141445, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001478 DASPLT010000000
VERSION     DASPLT010001478.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1081)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1081)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1081
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_141445"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            142..>1081
                     /gene="tig"
                     /locus_tag="VEA63_15115"
     CDS             142..>1081
                     /gene="tig"
                     /locus_tag="VEA63_15115"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HYD85394.1"
                     /translation="MNVQTNNVSDTRKSLVVTLDAAEVDATHQAVVKEFSELARIPGF
                     RPGKAPAAMVAKRFAKEIGDEFKQKVLAKAYREGLEQQKLDVLNIVNVEAGDVKAGEP
                     VTVTVTLDIRPEFALPEYVGLPTEVAPADVSDADVEKVIEGMRAERAEFKVAERAAAK
                     GDYVKLAYEGKIDGQPIADLAPDKQIYGKVPQTWEEVEGANEGVIPGLGQQLAGLKPG
                     DKKDVTVNFPADFAAVPSLAGKTAVYSVEVQEIRERVLPPMDEEFFKAHKVGSLETLQ
                     VAVRSQLKRQKEAQNRAEQRRQVSEAIVGKVDFPVPE"
BASE COUNT          226 a          294 c          382 g          179 t
ORIGIN      
        1 cgaaagctgg gcatcgctat ttcgtcacca aacggcgcgg cgcgccctcg tgcaagcgcg
       61 cagaatccgg cattgaccac cccgggctgc gacctagcgt cgacgattct ttatctcgaa
      121 caacccgccg catttcttcc tgtgaacgtc caaacgaaca acgtctccga tacccgcaag
      181 agcctcgtcg tcactctcga cgcggctgaa gtcgacgcca cgcatcaggc ggtggtgaag
      241 gagttttccg aactcgcccg cattccgggt ttccgtccgg gcaaggcgcc ggcggcgatg
      301 gtggcgaagc gtttcgcgaa ggagatcggt gacgagttta agcagaaggt gctcgcgaag
      361 gcctatcgcg aaggactcga gcagcagaag ctcgatgtgt tgaacatcgt gaatgtcgaa
      421 gcgggcgacg tgaaggcagg cgaaccggtg acggtgacgg tgacgctgga tatccggccg
      481 gagtttgcgc tgccggagta tgttggtttg ccgacggaag tcgcgccggc ggacgtgagc
      541 gatgcggacg tggagaaagt catcgagggc atgcgcgcgg agcgtgcgga gttcaaagtg
      601 gccgagcgcg ccgcggcgaa gggcgactac gtgaagctcg cttacgaggg caagatcgac
      661 ggccagccga tcgcggattt ggcgccggac aaacagattt acggcaaggt cccgcagacg
      721 tgggaagaag tcgaaggcgc caacgaaggc gtgattcccg gactcggtca gcaactcgcg
      781 ggcctcaagc cgggcgacaa gaaggacgtg acggtgaact ttccggcgga cttcgcggcg
      841 gtgccgtcgc tcgcgggcaa gacggcggtt tattcggtcg aggtgcagga gattcgcgag
      901 cgggtgttgc cgccgatgga cgaagaattt ttcaaggcgc acaaggtcgg cagcttggaa
      961 acgctgcagg tcgcagtgcg cagccagttg aaacgtcaga aggaagcgca gaaccgcgcc
     1021 gagcagcggc gtcaggtttc ggaggcgatc gtcggcaaag tggatttccc ggtgccggag
     1081 t
//
LOCUS       DASPLT010001479         1082 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_242002, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001479 DASPLT010000000
VERSION     DASPLT010001479.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1082)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1082)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1082
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_242002"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            141..683
                     /gene="sufT"
                     /locus_tag="VEA63_15120"
     CDS             141..683
                     /gene="sufT"
                     /locus_tag="VEA63_15120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009514297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative Fe-S cluster assembly protein SufT"
                     /protein_id="HYD85395.1"
                     /translation="MSNHILSRETQATMIPAGDVVTLPAGSEVFITQTLGGNVTVRTD
                     GGLFRIANSDADAIAGYVPAPAASKAAAAPGGEFSEKAVWEVLKTCFDPEIPVNIVDL
                     GLVYDLASEKTESGGYQVEVKMTLTAPGCGMGPVIAEDARQKIAALPQVEVAKVHIVW
                     DPVWTPQMISPEGRLALGLE"
     gene            complement(756..>1082)
                     /locus_tag="VEA63_15125"
     CDS             complement(756..>1082)
                     /locus_tag="VEA63_15125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009513183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase family protein"
                     /protein_id="HYD85396.1"
                     /translation="VTLADVRDYIERLYRETQTPGLLERFHVNKMKKYLNFVGQSVDP
                     AGAFLHDMRRAETERDLFAICDRHLLSDPSREFAPEPYAGVIARPNCETPEAAPDGCS
                     LETITA"
BASE COUNT          209 a          270 c          404 g          199 t
ORIGIN      
        1 ctcggaaaga tcacggcgcg cgtcgagcag gagttgaaga cgatccagtc gttcaacgat
       61 ccgcagggcc tttatgcgcg actgccggtc aatcttgcga tccagtaact ggcgaaccga
      121 aacggggaga aatttttttc atgtccaatc acattctttc gagggaaacg caggcgacga
      181 tgattccggc gggcgacgtg gtgacgctgc cggcgggcag cgaggtgttc atcacgcaga
      241 cgctcggcgg caacgtgacg gtgcgcacgg acggcggctt gtttcgtatc gcaaattcgg
      301 atgcggatgc gatcgcaggc tacgtgccgg caccggctgc gtcgaaagcg gcggcggcgc
      361 ccggcggtga gttcagcgag aaggcggtgt gggaggtgtt gaagacgtgt ttcgatccgg
      421 agattccggt gaacatcgtc gacctcggat tggtgtatga tctcgcgagc gagaagaccg
      481 agtcgggtgg ttatcaggtc gaggtgaaga tgacgctgac ggcgccgggc tgcggcatgg
      541 ggccggtgat cgcggaggac gcgcggcaga aaatcgcggc gctgccgcaa gtggaggtgg
      601 cgaaggtgca catcgtgtgg gatccggtgt ggacgccgca gatgatttcg ccggaggggc
      661 gattggcgct ggggttggag tgagggacac ggaagaccgg gcgtgagtcc aaaaacggac
      721 ggctcggaga gccgtcccta ccttgatctc tgcgatcagg cggtgatcgt ttcgagggag
      781 cagccgtcgg gggcggcttc gggcgtctcg cagttcggtc gggcgatcac gccggcgtaa
      841 ggctcgggcg cgaactcgcg ggatggatcg ctgaggagat ggcggtcgca gatggcaaaa
      901 agatcgcgct cggtttccgc gcgacgcatg tcgtgaagaa aagcacccgc agggtcgacg
      961 ctctggccga cgaagttcag gtatttcttc atcttgttga cgtgaaaacg ctcgagcagg
     1021 ccgggcgttt gggtctcgcg gtaaaggcgt tcaatgtagt cgcgaacgtc ggccaacgta
     1081 ac
//
LOCUS       DASPLT010001480         1433 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_161063, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001480 DASPLT010000000
VERSION     DASPLT010001480.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1433)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1433)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1433
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_161063"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..268)
                     /locus_tag="VEA63_15130"
     CDS             complement(<1..268)
                     /locus_tag="VEA63_15130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HYD85397.1"
                     /translation="MTPRKKLIAGNWKMNKTSADAFALVSEIVAAAGKINDVDVVVCP
                     PFTSLESAGKALEGSSVKLGAQNMHPEAGGAFTGEISAPMLRAIF"
     gene            complement(390..>1433)
                     /gene="pgk"
                     /locus_tag="VEA63_15135"
     CDS             complement(390..>1433)
                     /gene="pgk"
                     /locus_tag="VEA63_15135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HYD85398.1"
                     /translation="RSAKFSLRPVAAELEKLLGRPVKFLDDCVGPAVEAACAPGALKP
                     GEVVLLENLRFHIEEEGKVKLEDGSSKKADAKAIEAFRASLTKLGDVYVNDAFGTAHR
                     AHSSIVGVNLPQKAAGFLMEAELKAFSKVLESPDRPLLAILGGAKIADKIPLIKNLIE
                     KADKIIIGGGMAYTFYKQLHGVKIGNSLFDAEGAKIVGELVEKAKARGVELIFPVDFI
                     AADKFAPDANTQPADLKTGIPDGWEGLDAGPKSIELYRKAILDSKTIVWNGPAGVFEF
                     EKFAGATKAMADAIAEATEKGATTIVGGGDTATAAKKFGVAKKVTHCSTGGGASLEFL
                     EGKVLPGVAFLES"
BASE COUNT          249 a          472 c          413 g          299 t
ORIGIN      
        1 caaaaatcgc ccgcagcata ggcgcggaaa tttcgccggt aaacgcgccg cccgcctccg
       61 ggtgcatgtt ctgcgcgccg agtttgaccg aggaaccctc gagcgccttt ccggcggatt
      121 cgagcgacgt gaacggcgga catacgacga cgtcgacgtc attgatcttg ccggccgcgg
      181 cgacgatttc gctgacgagc gcgaaggcgt cggccgacgt cttgttcatc ttccagtttc
      241 cggcgatcag ttttttgcgt ggtgtcatgg cgaagtagct aaagtttaga gttctgagtt
      301 tcgagttccg agttgagagc aggtttcgcg attagagctc cgtcggtcca acccgaaacg
      361 cggagccttg aaccgaaacc gcgagcgcgt cagctctcga gaaacgccac gcccggcagc
      421 acttttcctt ccaaaaattc gaggctcgcg ccgccgccgg tggagcaatg cgtgactttc
      481 ttcgccacgc cgaatttctt cgccgccgtc gccgtgtcgc cgccgccgac aatcgtcgtt
      541 gcgcctttct cggtcgcctc ggcaatcgca tcggccatcg ccttcgtcgc gcccgcgaac
      601 ttctcgaatt cgaacacgcc cgcgggaccg ttccacacga tcgtcttcga atcgaggatc
      661 gccttgcgat agagctcgat cgacttcggg ccggcatcga ggccttccca gccgtccgga
      721 atacccgtct tcaaatcggc cggctgcgtg ttcgcgtccg gcgcgaactt gtccgccgcg
      781 atgaaatcga ccgggaagat caattcgacg ccgcgcgctt ttgctttctc gacgagctcg
      841 ccaacgatct tcgcgccttc ggcatcgaag aggctgttgc cgattttcac gccgtggagc
      901 tgcttgtaga acgtgtaagc catgccgccg ccgatgatga ttttgtcggc tttttcgatc
      961 aggtttttga tcagcggaat cttgtcggcg atcttcgcgc cgccgagaat cgcgagcagc
     1021 gggcgatcgg ggctctccaa aactttggag aacgccttca actccgcttc catcagaaaa
     1081 cctgcggctt tctgcggcag gttcacgccg acgatcgacg aatgcgcgcg gtgcgcggtg
     1141 ccgaacgcgt cgttgacgta cacgtcgccc agtttcgtca gcgacgcgcg gaacgcctcg
     1201 atcgctttcg catcggcctt cttcgacgaa ccgtcctcga gcttcacctt gccctcctcc
     1261 tcgatgtgga agcgcaggtt ctccagcagc acaacctcgc ccggtttcag cgcacccggc
     1321 gcgcaggcgg cctcgaccgc ggggccgacg caatcatcga ggaacttcac cggccgcccg
     1381 agcagcttct ccagttcggc cgcaaccggc cggagcgaaa actttgcgct gcg
//
LOCUS       DASPLT010001481         1067 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_283363, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001481 DASPLT010000000
VERSION     DASPLT010001481.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1067)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1067)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1067
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_283363"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            174..458
                     /locus_tag="VEA63_15140"
     CDS             174..458
                     /locus_tag="VEA63_15140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009513676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD85399.1"
                     /translation="MKSAYELAMERLAKSDPDTGKALTPEQKGRLAEIDRVYQGKIAE
                     REIFLKKQLNDALAQQNADEYQKIQQQIVSERARLEEEREEEKERVRRAK"
     gene            complement(811..>1067)
                     /locus_tag="VEA63_15145"
     CDS             complement(811..>1067)
                     /locus_tag="VEA63_15145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007360180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HYD85400.1"
                     /translation="EQLAASRLPTILTGDFNVRRHEELIAQFMKQGFSTVQQTEPTWR
                     RRAYVLDHIFYNAPLRPVRHMVAPTLASDHHAIVADFAFG"
BASE COUNT          244 a          326 c          305 g          192 t
ORIGIN      
        1 gcagaaggcg caaagaagat caatcgcgcc tcggaacgcg ctttcgggtg tggtattttt
       61 tgcgcctttt gcgccttttg cgccttcttg cggccaagtg cgtcgaagcg ccgaaaccgc
      121 cgcgacgctt tatttcgtgt ctttcgtgag tttcgtggtt caacactcct cccatgaaaa
      181 gcgcctacga actcgccatg gaacgcctcg ccaaatccga tccggacacg ggcaaggcgc
      241 tcacgccgga acaaaaaggc cggctcgccg aaatcgatcg cgtctaccag ggtaaaatcg
      301 ccgaacgcga aattttcctg aagaagcagt tgaacgacgc gctggcccag cagaacgccg
      361 acgaatatca gaagatccag caacagatcg tgagcgaacg cgcgcgtctc gaagaagagc
      421 gcgaagagga aaaagaacgc gtccgccgag caaagtaggg cgggcttcag cccgcctttc
      481 cggctcgccc gtagcggcag gcctccgtgc ctgcctgctt ctgtagattg cgcgcccgcg
      541 cgcaactact cgcccgcacg gaggcacatt ccgaacgatc agcagacagg cacggaggcc
      601 tgccgctacg catacgcatc gagctcagcc gaacgcatgc aaagagaaac cacgaatcga
      661 cacgaatgaa cacgaattca gagaagccga aggaaagctt cggcgctgat cgtcacgtca
      721 accttcggtg ttccgataag gccccgcatt cgaacgcgat tcattcgtgc cgattcgtgt
      781 ccattcgcgg ttcaaatgaa ttgttccggt tcagccgaac gcaaaatccg ccacgatagc
      841 gtggtgatcc gacgcgagcg tcggcgccac catgtgacgc accggacgca gcggcgcgtt
      901 gtagaaaatg tgatcgagca cataggcgcg gcgccgccat gtcggctcgg tttgttgcac
      961 cgtcgagaaa ccctgcttca tgaattgtgc gatcagctcc tcgtggcgtc gcacattgaa
     1021 atcgcccgtg aggatcgtcg gcagccgcga ggccgccagt tgctccg
//
LOCUS       DASPLT010001482         1155 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_18249, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001482 DASPLT010000000
VERSION     DASPLT010001482.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1155)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1155)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1155
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_18249"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..670
                     /locus_tag="VEA63_15150"
     CDS             <1..670
                     /locus_tag="VEA63_15150"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HYD85401.1"
                     /translation="FHVQDTGGGIPAEILPHIWEPFFTTKATGKGTGLGLSTVRGIVE
                     SHAGFTTIETVPNKGTTFRVYLPAAESTSLAPGAAATSTVMPRGTGELVLVVDDEPSI
                     RNMAAAMLSRHGYRVLTAADGAEAVALFAPRSKEIRVVITDLSMPNLDGAALASVVHR
                     LNPLVKIIAMSGLTPQMENERPAPQFTHAFLTKPFRPELLLTMVYDLLRAPPNPPPPS
                     PSPA"
     gene            942..>1155
                     /locus_tag="VEA63_15155"
     CDS             942..>1155
                     /locus_tag="VEA63_15155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007362735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HYD85402.1"
                     /translation="MSNSKLYVGNLSFKTTEDELRSAFGQFGSVTDVYVAMDKMTGRP
                     RGFAFVTMGTADEAKSAAEKLNGTDLG"
BASE COUNT          229 a          404 c          317 g          205 t
ORIGIN      
        1 gtttcacgtg caggacaccg gcggcggcat cccggcggaa attctgccgc acatctggga
       61 gcctttcttc accaccaaag ccaccggcaa aggcaccggc ttggggctct ccaccgttcg
      121 cggcatcgtc gaaagccacg ccggtttcac gaccattgag accgttccca acaaaggcac
      181 aactttccgc gtctaccttc ccgccgccga aagcacctcg ctcgcgcccg gcgctgctgc
      241 cacctcgacc gtcatgccgc gcggcaccgg tgaactcgtc ctcgtcgtcg acgacgaacc
      301 gagcatccgc aacatggccg ctgcgatgct ctcgcgtcac ggctaccgcg tgctcaccgc
      361 cgcggacggc gcggaggccg tcgcgttgtt cgcgccgcga agcaaggaga tccgcgtcgt
      421 catcaccgat ctcagcatgc ccaacctcga tggcgccgcg ctcgccagcg tcgttcaccg
      481 gctcaaccct ttggtgaaga tcatcgcgat gagcggcctc actccacaga tggaaaacga
      541 gcggcccgcg ccgcagttca cgcacgcgtt tctcaccaaa ccgttccgtc ccgagctcct
      601 gctcacgatg gtctacgatc ttttgcgcgc accgccaaat cccccgccgc cctcgccatc
      661 gccagcgtga aacgccgctg caaaccgcag gcccgggccg actcacacac ctgctcacca
      721 ccgccacaga cccgcgcttg cgagcgctgc agcaggccga caaatcgcga attcccttgc
      781 atttatccga accgcgtgcg tccttgcggc gcatcgttag agacagcgtt agatgacagt
      841 atctggtgag tcgtaggctg agcgataggc ttaagtgatg atttcgaaac ccacggtggg
      901 aacggactgg cagcagacga aggatctacg agacatcact aatgagtaat tccaaactat
      961 acgttggaaa tctgtcgttc aagaccaccg aagacgagct ccgctcggcg ttcggccagt
     1021 tcggttcagt taccgacgtt tatgttgcca tggacaagat gaccggccgc ccccgcggtt
     1081 tcgcgttcgt caccatgggc accgccgatg aggccaagtc cgcggcggag aagctgaatg
     1141 gcaccgatct cggtg
//
LOCUS       DASPLT010001483         2776 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_98656, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001483 DASPLT010000000
VERSION     DASPLT010001483.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 2776)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2776)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2776
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_98656"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2776
                     /locus_tag="VEA63_15160"
     CDS             <1..>2776
                     /locus_tag="VEA63_15160"
                     /inference="COORDINATES: protein motif:HMM:TIGR02608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="delta-60 repeat domain-containing protein"
                     /protein_id="HYD85403.1"
                     /translation="GINTIALQGDGKILLGGEFTQVAGHGANTSFNRLRLARLNANGT
                     LDESFNVNVDQPVFTLFVQADNKIVVGGSFGLVAVNNAATATARNSLVRLNADGTIDE
                     DFDPNPNGPVFSVAQVDSKLVIGGRFTTLQPDKKEDGLRTRNFVARLEANGDLDQGFN
                     LDLDTLPGNQVVKVAAAGEGKLLVAGAFSTLNSSDAATFLRRERLARVNADGKVDTGF
                     DAGLGSALGAPIESLTVQSDGRLIAAGDFTGFGGTAATNLARFNLDSSPDTSFTPAVN
                     GPVYAVAQQLTKGEPVATPRAGFGVLENNGQLRTTFAIEANVTLPTIYTVVVQPDGKL
                     LIGGTNFTSPITGTSVGSLLRLNTNGTLDAAFAPKTNGEIFAIAPLSDGDILVGGTFT
                     KVGDVDRTNIARLNSDGSLDTAFTLNVNSTGPVYAIAVQSDGRIVLGGGFTSIQTSPT
                     STTFTRTYAARIANDGTVDGAFDPGFNSTVYSILIRPEGKILLSGAFTGLRPNGATTT
                     TDRTYAAQFNADGTFDTAFDLKLNGSANELALDSNNEVLIGGYFTRILGQTRNQLARI
                     NADNTLDAFNPNPNNAVTSIAVQPDGKILVSGLFNALQPNSETYDPSLAVPRNRAVRL
                     NADGTIDPTFNPNFNSTVAGLVPGSENTVVAFGSFNALQPTGGLVVGGEFSAVNGIPV
                     SNLALFGRDGSVSATFAPNPNGAVRAILPMTNGQTVVAGSFTTISGATRNRVARFTAA
                     DELDAGFNPNVDGGDVLSIALQADGKLVIGGSFTSVGGAARANLARLNTDGSLDGSFA
                     PTITGAVNNIVVQPDGRLLITQTTGADTNSLVRLNASGSPDASFNAANNGKVNGVALQ
                     VSGHIVVGGNFTSIGGGNRSNLARLNPNGTLDAAFTSTPQGAVTALSIQPDGKILIGG
                     QFNSVDNIAR"
BASE COUNT          518 a         1040 c          766 g          452 t
ORIGIN      
        1 ccgggataaa cacgatcgcg ctgcaaggcg acggcaagat tctcctcggc ggcgagttca
       61 cgcaggtcgc cggccacggc gcgaacacct ccttcaaccg cctgcgtttg gcgcggctca
      121 acgcgaacgg caccctcgac gaaagcttca acgtcaatgt cgaccagccg gtgttcaccc
      181 ttttcgtcca agccgacaac aagatcgtcg tgggcggatc gttcgggctc gtcgctgtga
      241 acaatgccgc gaccgccacc gctcggaact ctctcgtgcg gctcaatgcc gacggcacga
      301 tcgacgaaga cttcgatccg aatccgaacg ggccggtctt ttccgtcgca caggtcgact
      361 cgaagctcgt gatcggcggc cgattcacga cgctgcaacc cgacaagaag gaagacgggc
      421 tccgcacgcg caatttcgtc gcgcgcctcg aagccaacgg cgacctcgac cagggcttca
      481 atctcgacct cgacacgctg ccgggcaacc aagtcgtcaa ggtcgccgcc gcgggtgaag
      541 gaaagctcct cgtcgccggc gcgttttcca cgctcaacag ctcggatgcc gccacattcc
      601 ttcgtcgcga gcgcctcgcg cgggtgaacg ccgatggcaa ggtcgatacg gggttcgatg
      661 ccggactcgg cagcgcactc ggcgctccga tcgaatcgct gaccgtgcaa tcggacggcc
      721 gtctgatcgc cgcgggcgac ttcaccggct tcggcggcac cgccgcgacc aatcttgcac
      781 gcttcaatct cgatagctcg cccgacacct cgttcacccc cgcggtaaac ggacccgtct
      841 acgccgtcgc gcagcagttg accaagggcg aaccggttgc cacgccgcgt gccggtttcg
      901 gcgttttgga aaacaatggc cagctccgca ccactttcgc gatcgaagcc aacgtcaccc
      961 tgcccacgat ctacaccgtt gtcgtgcaac cggacggcaa gctgctgatc ggcggcacca
     1021 acttcaccag ccccatcacc ggcacgtccg tcgggagtct cttgcggctc aacaccaacg
     1081 gcacgctcga tgcggccttc gctccgaaaa cgaacggcga aatcttcgcc atcgcgccac
     1141 tttcggacgg cgacattctg gtcggcggca cgttcaccaa ggtcggcgac gtcgaccgca
     1201 cgaacatcgc gcggttgaac tccgacggct cgctcgacac cgccttcacg ctgaacgtca
     1261 acagcaccgg ccccgtctac gcgatcgccg tccagtccga cggtcgcatc gtgctcggcg
     1321 gcggtttcac ttcgatccag acgtcgccca cctccacgac gttcacgcgc acttacgctg
     1381 cgcgcatcgc caacgacggc acggtggacg gcgcgttcga tccgggtttc aactccaccg
     1441 tctacagcat tcttattcgc cccgagggga aaatcctcct ctccggcgcc ttcaccggct
     1501 tgcggcccaa cggcgcgacg accaccaccg atcgcaccta cgccgcccag ttcaacgccg
     1561 acggcacgtt cgacaccgcg ttcgacctga agctcaacgg ctcggccaac gagctcgcgc
     1621 tcgattcgaa taacgaggtg ctcatcggcg gttacttcac ccgcatcctc ggccagaccc
     1681 gcaaccagct cgcgcgtatc aacgccgaca atacgctcga tgcgttcaac ccgaatccga
     1741 acaacgcggt caccagcatc gccgttcaac ccgacggcaa gatcctcgtc tccggcctgt
     1801 tcaacgcact gcagcccaac agcgaaacct acgatccgtc cctcgccgtg ccgcgcaacc
     1861 gcgccgtgag actgaacgcc gacggcacca tcgacccgac cttcaatccg aacttcaaca
     1921 gcaccgtcgc cggcctcgtc cccggcagcg agaacaccgt ggttgcgttt ggctccttca
     1981 acgccctgca gccgaccggc ggcctcgtgg tcggcggcga gttcagcgcg gtcaacggca
     2041 tccccgtcag caatctcgcg ctgttcggcc gcgatggctc cgtcagcgcg acgttcgccc
     2101 cgaatccgaa cggcgccgtg cgcgcgatcc tgccgatgac caacggacag accgtcgtcg
     2161 ccggctcgtt caccaccatc tccggtgcga cacgcaatcg cgttgcccgc ttcaccgccg
     2221 cggacgaact cgatgccggc ttcaatccga acgtcgacgg cggcgacgtc ctttcgatcg
     2281 cgctgcaggc cgatggcaag ctcgtgatcg gcggcagttt caccagcgtc ggcggcgccg
     2341 ctcgcgccaa cctcgcgcgg ttgaacaccg acggttcgct cgacggctct ttcgccccca
     2401 ccatcaccgg cgcggtgaac aacatcgtcg tccagcccga cggccgtctg ctcatcacgc
     2461 aaacgaccgg tgccgacacc aacagcctcg tccgcctcaa cgccagcggc agccccgacg
     2521 ccagcttcaa cgccgccaac aacggcaagg tcaacggcgt cgcgcttcaa gtgagcggtc
     2581 acatcgtcgt cggcggcaac ttcaccagca tcggtggcgg aaaccgctcg aatctcgcgc
     2641 ggttgaatcc caacggcacg ctcgatgcgg cgttcacgtc gacgccccaa ggcgctgtca
     2701 ccgctttgtc gatacagccg gacggcaaga ttctcatcgg cggccagttc aactccgtcg
     2761 acaacatcgc gcgctt
//
LOCUS       DASPLT010001484         1165 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_161163, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001484 DASPLT010000000
VERSION     DASPLT010001484.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1165)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1165)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1165
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_161163"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..237
                     /locus_tag="VEA63_15165"
     CDS             <1..237
                     /locus_tag="VEA63_15165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012377006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HYD85404.1"
                     /translation="DKLVALQELAASLNLTAADCVYMGDDDIDASAIAWAAIGATVPD
                     AMPSALAAADYVTTRPAGRGAVREICENILAARR"
     gene            254..799
                     /locus_tag="VEA63_15170"
     CDS             254..799
                     /locus_tag="VEA63_15170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD85405.1"
                     /translation="MTSHRKQTAHRQQRSRTGYGAVTARPPRQPHALHGPIAPTLTFT
                     FLLIFTFTLPLPSSAAEVTAPARNWVLPVFTPEGFREAIGRGSEARARDDHTFEVVDL
                     NLTFFSGDAANRVDTVILSPAATFLPDQKIARGEKFVRFIRDDLEASGIRWVYNHADK
                     KISLDGNVRVTFRAEIDDLLR"
     gene            796..>1165
                     /locus_tag="VEA63_15175"
     CDS             796..>1165
                     /locus_tag="VEA63_15175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009508625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD85406.1"
                     /translation="MNLLRSLLALTFAACAAVLAHAADTPQATALSCDHMDMWSEGDE
                     TKAICTGNVIVTGTNLRIVADRLELTASRIDDEKNESVPTLEKFRYLLATGNVSITQG
                     TRTATCGRAEVLPREEKVVLT"
BASE COUNT          210 a          483 c          279 g          193 t
ORIGIN      
        1 gacaagctcg ttgccctcca ggaactcgcc gcctcgctca atctcaccgc cgccgactgc
       61 gtttacatgg gcgacgacga catcgacgcg tccgcgatcg cctgggccgc catcggcgcc
      121 accgtccccg acgcgatgcc ctccgcgctc gccgccgccg actacgtcac gacgcgcccc
      181 gccggccgcg gcgccgttcg cgaaatctgc gaaaacatcc tcgccgcccg ccgatgaccc
      241 cgcacggaga acgatgacct cgcaccgaaa acagaccgcg caccgccaac aacgtagccg
      301 caccggttat ggtgcggtca ccgcccgccc tccacgccag ccgcacgccc ttcacgggcc
      361 gatcgcgccc accctcactt tcacgttcct cctcatcttc accttcaccc tccctctgcc
      421 ttcctccgcc gccgaggtca ccgcccccgc gcgcaactgg gttcttcccg ttttcacccc
      481 cgaaggcttc cgcgaagcca ttggccgcgg ctccgaagct cgcgcccgcg acgaccatac
      541 cttcgaagtc gtcgatctta acctcacgtt tttcagcggc gacgccgcga atcgtgtcga
      601 caccgtcatc ctcagccccg ccgccacgtt tcttcccgat caaaaaatcg cgcgcggcga
      661 aaaatttgtc cgcttcattc gcgacgacct cgaggcctcc ggcattcgct gggtctataa
      721 ccacgcggac aaaaagattt cgctcgatgg caacgtccgc gttaccttcc gcgccgagat
      781 cgacgatctc ctccgatgaa ccttctccgc tcgctcctcg ccctcacgtt cgctgcctgc
      841 gccgccgtcc ttgcgcacgc cgccgacacg cctcaagcca ccgcgctctc ctgcgatcac
      901 atggacatgt ggagcgaggg cgacgaaacg aaggccatct gcacgggcaa cgtcatcgtc
      961 accggcacca atctccgcat cgtcgccgac cgcctcgagc tcaccgcctc tcgcatcgac
     1021 gacgagaaaa acgaatccgt tcccacgctc gaaaaattcc gctacctgct cgccaccgga
     1081 aacgtctcga tcacccaagg cacccgcacc gccacctgcg gccgagccga agttctccct
     1141 cgcgaagaga aggtcgtcct caccg
//
LOCUS       DASPLT010001485         2881 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_98703, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001485 DASPLT010000000
VERSION     DASPLT010001485.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 2881)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2881)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2881
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_98703"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1059)
                     /locus_tag="VEA63_15180"
     CDS             complement(<1..1059)
                     /locus_tag="VEA63_15180"
                     /inference="COORDINATES: protein motif:HMM:NF021446.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2157 domain-containing protein"
                     /protein_id="HYD85407.1"
                     /translation="MKRFEQQLAEHSSEWVRDGLITSEQRAALIARHPAEESHRFIAI
                     LGTIGGALVAAGIGLLIAANWQQLDDWIKIVGLVALLAGAHVTGWRLRISPGHFPKTG
                     EVFFMIGCVLFLLGIALVSQIFHLNSRPASGVLLWWLGIAALPWIVRVKSAQCVSVVA
                     LLVWLAMELAAKDSWLRLSAAETWNSGELYAVAFFLLGSSLLFTGVALRRCAHAEFAG
                     IHEKLGLLVANGALFALTFSWNKHLWNTTQAADVRLMPFGVFAALLLAAAAWAWRSNA
                     ADTARIAGWIGVGVVPALGFVVGLIGYGAAWWWGAAACVALFFLNVGMIRTGLATGRE
                     SWINLGVAFVALNIIARYI"
     gene            complement(1056..1703)
                     /locus_tag="VEA63_15185"
     CDS             complement(1056..1703)
                     /locus_tag="VEA63_15185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD85408.1"
                     /translation="MNVAILSESPADEAAVRVLAEAVLKAPIRQVQGALRARGWPNVV
                     QILPAVVRHLHFNTDAAGLVVVVDSDDSVVHTIDHEAPEYHHPLCRVCQVRAVFRRAT
                     KKLPPARGRNRVLRGVGVAVPAVEAWYLCGRDSSVTEAAWIEGQERGVAPYTRRELKW
                     RVYGTERPSLPHETQRALEEVRRFRGDVRRLEADFPGGFGALARDLRSWQDKPQQ"
     gene            complement(1902..>2881)
                     /locus_tag="VEA63_15190"
     CDS             complement(1902..>2881)
                     /locus_tag="VEA63_15190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HYD85409.1"
                     /translation="VAFRNFKALRSASVRLAPFNLVIGPNGSGKTSLIEALLHLRTLS
                     KLAPTDGDGHDEQRPDILFEFSPPHAGVRVRLSCIDSEMCDALRISPANAPEWPALRN
                     DIGRIRSYVFDHAAMSQPSVASNSTELSADGANLAAVIAALKTSEPDTYAAFVGEALR
                     LFPEFSAFKIQSRPGGAVALAFTLIGEVGLVDGENLSQGMLHTLGVLALAAQPTPPSV
                     LCIEEIDRGVHPRMLREVRDALYRLAYPGSFGLARPPVQVIATTHSPYLLDLFRDHPE
                     EVIVTQKHGREAHFERLSDRADLAEILQEGTLGDLWFSGILGGVPEEKS"
BASE COUNT          586 a          972 c          850 g          473 t
ORIGIN      
        1 gatgtaacgc gcgatgatgt tcaacgcaac gaacgccacg ccgaggttga tccagctctc
       61 gcgtccggtg gcgagtcccg tccggatcat gccgacgttg aggaaaaata gcgcgacgca
      121 cgctgccgca ccccaccacc aggccgctcc atagccgatg aggccgacaa cgaagcccaa
      181 tgccggcacg acaccgacgc cgatccaacc ggcgatccgc gctgtatccg ccgcgttgct
      241 acgccaggcc catgccgccg ccgcgaggag caacgccgcg aagacgccaa acggcatcaa
      301 ccgaacgtcg gcggcctgcg tcgtattcca caggtgcttg ttccaactga aggtcagcgc
      361 gaaaagcgcg ccattcgcta cgagcagtcc aagcttttca tgaatgcccg cgaattccgc
      421 atgcgcgcat cgacgcagcg cgaccccggt gaacagcaac gacgagccca ggagaaaaaa
      481 cgccaccgca tacagttccc cggagttcca ggtctcggcc gcgctcagcc gcagccagga
      541 atccttcgcc gcgagttcca ttgcgagcca caccaacagc gccaccacgc tcacgcactg
      601 cgcgctcttc acccgcacga tccacggcag cgcggcgatg ccgagccacc agagcagcac
      661 accgctcgcg ggacggctgt tgaggtgaaa aatctgactc accagcgcga tcccgagcag
      721 gaaaagcacg cagccgatca taaagaacac ctcgccggtt ttcggaaaat ggccgggaga
      781 gatgcgcaac cgccacccgg tcacgtgagc gccagcgagc aacgcgacga ggcccacgat
      841 tttgatccag tcgtcgagtt gctgccagtt cgccgcgatg aggagaccga tgccggctgc
      901 aaccagcgct ccgccaatcg tgccgaggat cgcgatgaag cggtgactct cctcggcggg
      961 atgacgcgcg atcaacgccg cccgctgctc cgaggtgatc agcccgtcgc gcacccattc
     1021 gctcgaatgc tccgcgagtt gttgttcgaa gcgcttcatt gctgcggctt gtcctgccag
     1081 ctccgcaggt cccgggcaag cgcgccgaag cctccgggaa aatccgcctc gagtcggcgc
     1141 acgtcgccgc gaaaacgacg cacttcctcc agcgcccgct gcgtttcatg cggcagactc
     1201 ggccgctccg tgccgtaaac gcgccacttc aactcgcgtc gcgtatacgg cgcgacgcct
     1261 cgctcctgcc cctcgatcca tgccgcctcc gtgacgctgc tatcgcgtcc gcaaagatac
     1321 caagcctcca ccgcaggcac ggcgacccca acgccgcgca gcacgcgatt ccgcccgcgg
     1381 gccggcggca gtttttttgt cgcacggcga aacacggcgc gcacctggca cacacggcac
     1441 agcggatggt gatactccgg cgcctcgtga tcgatcgtat gcacgaccga gtcatcggaa
     1501 tcgaccacga cgacgagccc cgccgcgtcc gtgttgaaat gcagatgacg aacgaccgcc
     1561 ggcagaatct gcacgacgtt cggccaacct cgcgctcgca acgccccctg cacctggcgt
     1621 attggagctt tcaatactgc ctccgccagc acgcgcacgg ccgcctcatc cgccggcgat
     1681 tcgctgagaa tcgcaacgtt catgctgaat gaaccgcgga tgaaccaaga tgaacacggt
     1741 tttggatcgg acccggtcca ttttgaacca cgaatggaca ccaatgcaca cgaatgaaat
     1801 gcccggcgga tcgttgcctt gggtttctat tcgtgtgcat tcgtgtccat tcgtggtttc
     1861 agctcagccc gtaatccccg tttatcggtg tgcatccgtg gttagctttt ttcctccggc
     1921 acgccaccga ggatgccgct gaaccagaga tcgccgagcg tcccttcctg tagaatttca
     1981 gcaaggtcgg cgcgatcgga gagccgctcg aaatgcgctt cgcgaccgtg cttctgcgtc
     2041 acgatcactt cctccggatg atcgcgaaaa agatcgagca gatagggaga atgcgtggtt
     2101 gcgatcacct ggaccggcgg acgcgcgagt ccgaacgacc cgggataggc gagccggtaa
     2161 agggcgtcgc gaacttcgcg caacatccgc ggatgcacac cgcgatcgat ctcttcgatg
     2221 cacaacacgc tcggtggcgt cggctgcgcc gcaagtgcga gcacaccgag cgtgtgcagc
     2281 atgccttgcg aaagattctc gccatcgacg agcccgactt cgccgatcaa cgtgaacgcg
     2341 agcgcgaccg cgccgcccgg ccgcgactga attttgaaag cggaaaactc cggaaaaagc
     2401 cgcagcgctt ccccgacgaa tgcggcgtag gtatccggtt ccgaggtttt cagcgcggcg
     2461 atcaccgccg cgagattcgc tccatcggcg gagagctccg tcgagttgct cgcgaccgac
     2521 ggctgcgaca tcgccgcatg atcgaaaacg tagctgcgaa tgcggcctat gtcgtttcgc
     2581 agcgcgggcc actcgggagc gttggcgggc gagatgcgca acgcgtcgca catctccgaa
     2641 tcgatgcaac tgagccggac ccgcactccc gcatgcggcg gggaaaattc aaagagaatg
     2701 tcgggacgct gttcgtcgtg tccgtcgccg tcggtcggcg cgagtttcga aagcgtgcgc
     2761 agatgaagca gggcttcgat gagactggtt ttgccgctgc cgttcggccc gatcacgagg
     2821 ttgaacggcg cgagtcgcac gctcgcactg cgcagggcct tgaaatttcg aaaggcgaca
     2881 g
//
LOCUS       DASPLT010001486         1986 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_202650, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001486 DASPLT010000000
VERSION     DASPLT010001486.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1986)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1986)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1986
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_202650"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1986)
                     /locus_tag="VEA63_15195"
     CDS             complement(<1..>1986)
                     /locus_tag="VEA63_15195"
                     /inference="COORDINATES: protein motif:HMM:TIGR01731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD85410.1"
                     /translation="VTAGENLHIRSTPGSTSATVTNQGSLVAGAGLTVDSEGSPVITY
                     RNVTGGVVGAQTVTIDAASFTNAGTIQGTRTLSVRTTGALSNEANALLITATETGRDV
                     TLDAGSFSNAGLVQSVGSLAVTVAGDARNSGSLGTLGSNDGGTDGQVTFTANTLDNSG
                     TIQSASTLDVTTSASAGVALANSGKLQAIDRATLTVGARFANAANGQVLAGSAMSIQS
                     GAGAFAMTNAGRLQAAQDLTIGSDSARVSYQQTADAVTLSGAAMQLRASTAENAGVLQ
                     AGDTLTIDTTGAFTNQVGGGVLTSGTPGKSVSLTAGSLTNAGQIQSTGSIIAAVAGAT
                     NNSGTILSLAADNAGADGDFSLRGDSVNNSGKIIAAGAVGIDVSRNFKNHENAAIVAA
                     RSLEATASTLQNNGALLAEGALALNTGSTLANAGLIQSLGDLRLVAPQLLTNATNAQI
                     LSGANLSLISDAGAWTLSNAGRIQSTGNLSLGAVNAVAAFQNQSTGIVFSSEALAIHA
                     SDLTNDGVIRGETEATFAVDGAFTNRHIVATDGHAGARLSIVSDIFANSGTGQIVSDG
                     SIEVAAAGGFNNAGTLLTQAANEGGSDEGISTRSSIFNNSGTFQSAGELEIVAHDKST
                     NSFANSGRLQAVDSISIVAPNRQEVTSTGVMLSGRNVT"
BASE COUNT          312 a          558 c          718 g          398 t
ORIGIN      
        1 cgtgacgttt ctgccgctga gcatcacgcc ggtgctggtg acttcctgtc ggttcggtgc
       61 gacaatcgag atggaatcca cggcttgcag tcgaccgctg ttggcgaagg aattggtcga
      121 cttgtcgtgg gcgacgatct cgagctctcc ggcggattgg aacgtgccgc tgttgttgaa
      181 gatgctgctt cgcgtcgaga tgccttcatc gctgccaccc tcgttcgcgg cctgagtcag
      241 cagggtgccg gcattgttga acccaccggc ggccgcgact tcgatcgagc catcggaaac
      301 gatctggccc gtgccgctgt tggcgaaaat atccgacaca atcgaaaggc gcgcgccggc
      361 gtggccgtcg gtcgcgacga tgtggcggtt ggtgaatgcg ccgtcgactg cgaacgtcgc
      421 ttcggtttcg ccgcggatca caccatcgtt ggtcaaatcc gacgcatgga tcgcgagcgc
      481 ttcgctggag aagacaatac cggtgctctg gttttggaac gcagcgacag cgttcactgc
      541 accgagggac agattgccgg tcgactgaat gcggccggcg ttcgaaagcg tccatgctcc
      601 ggcgtcggag atcaggctga gattcgcgcc actgagaatc tgcgcgttgg tcgcgttcgt
      661 gaggagctgc ggggcaacca agcggagatc gcccaacgat tgaatgaggc cggcgttggc
      721 gagcgtggaa ccggtgttaa gcgctagggc gccttcggcg agcagcgcgc cgttgttttg
      781 cagcgtggag gcggtcgcct cgagactgcg agcggcgaca attgcggcgt tctcgtggtt
      841 tttgaaattg cgtgagacgt cgatgccaac ggcgccggcg gcgatgattt tgccgctgtt
      901 gttcaccgaa tcgccgcgca gcgaaaagtc gccgtctgca ccggcgttgt cggcagcgag
      961 gctcaggatg gtgccgctgt tgtttgtggc gccggcgact gcggcgatga tgctgccggt
     1021 cgactgaatc tgtccggcgt tggtcaggga tccggccgtg agactgacgc tcttgccggg
     1081 cgtgccggat gtcagcacgc cgccgccgac ctggttggtg aacgcccccg tcgtgtcgat
     1141 ggtcaatgtg tcgccagcct gaagcacgcc ggcgttttcc gcggtcgatg cgcggagttg
     1201 catcgcagcg ccggacaggg tgacggcgtc cgcggtttgt tggtaactga cgcgggcgga
     1261 gtcggagcca atggtgagat cttgcgcggc ctggaggcgt ccggcgttcg tcatcgcgaa
     1321 cgcgccggcg ccggattgga tcgacatggc cgagccggcg aggacttgac cgtttgcggc
     1381 gttggcgaaa cgtgcgccca cggtgagcgt cgcgcgatcg atcgcctgaa gctttccgct
     1441 gttcgcgagt gcaacgccag cggatgcgct ggtggtcacg tcgagcgtgc tggcggattg
     1501 aatcgttcca gagttgtcga gcgtgtttgc ggtgaaggtc acttgaccat cggtgccgcc
     1561 gtcgttcgag ccaagcgtcc cgagtgagcc gctgttgcgg gcgtcacccg cgacggtgac
     1621 cgcgagcgag ccgaccgact ggaccaggcc ggcgttggag aacgaaccgg cgtcgagcgt
     1681 gacgtcgcgt ccggtttcgg tcgctgtgat cagaagcgcg ttggcttcat tgctcagtgc
     1741 gccggtggtg cgaacggaga gagtgcgcgt gccttggatc gtgccggcgt tagtgaaaga
     1801 cgcggcatcg atggtgacgg tttgcgcacc gacaacgccc ccggtgacgt tgcgatacgt
     1861 gatcacggga gaaccttccg agtcgacggt cagaccggcg ccggcgacga ggcttccctg
     1921 attggtcaca gtggcggagg tgctgccggg agtggagcgg atgtggaggt tttctccggc
     1981 ggtgac
//
LOCUS       DASPLT010001487         2781 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_119499, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001487 DASPLT010000000
VERSION     DASPLT010001487.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 2781)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2781)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2781
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_119499"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..825)
                     /locus_tag="VEA63_15200"
     CDS             complement(<1..825)
                     /locus_tag="VEA63_15200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HYD85411.1"
                     /translation="MKNYRAVLVGTGSIGEAHVRAVETTQGRVTLDAAVDIDATRVGE
                     FARRHAIPHAFTDYATMLAERRPDIVLVATPPAQHAPMSIAAMEAGAWVLCEKPLCGS
                     LAELDQILAAEQRTGCYTACIFQMRFASSTAHLKRLADNGHLGRPLVGVCNTLWYRDS
                     AYYAVPWRGRWETELGGPTMGLGIHAMDQFLHLMGDWTDVRAIARTLDRAIEVEDVSM
                     AIVSFANGAVGSIVNSALSPRQETYMRLDYQRATVELTHLYSYTRDNWKFTPVPPAQ"
     gene            complement(1181..2518)
                     /locus_tag="VEA63_15205"
     CDS             complement(1181..2518)
                     /locus_tag="VEA63_15205"
                     /inference="COORDINATES: protein motif:HMM:NF016212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF455 family protein"
                     /protein_id="HYD85412.1"
                     /translation="MAAPSFPAAAVSKPASSSTGRMAAVQDIARLLRVLFEIEREFVR
                     TATTLIYRVGDLDLKYLLCQHVWESASHARFLRERGRELSGFGKDENVRAELRQLFNE
                     AVMPADANVALAGFYRVLKPALLTAYRHYLEATHHLADWPSLRLVEEFIQDEERHARE
                     IAPFLGDATHDTTTWTEHLLTALAASGGFLGEKTSITLPAGFAWESSQRSYRHPDTCN
                     RGKFPTCSSVFSNDPDETPIVRSWLENPKTDARVIRLMVYVWLMMECDAVDYLATVFY
                     DTPNAPFDLHHDMARHLWDESRHSQFGYRQLPKLGVDLMTLEHSLDLYNILVQMPPHE
                     RYAMMTMEFEAGSFPTKATVMDRVRELNDFEADTLLAFDRNDEQNHVRYGHRWLPEIM
                     TLCGDTRSPEEFVKATRAHFAELSAIYGGRTPHALPPERRLTGNKILQLAGVR"
     gene            complement(2592..>2781)
                     /locus_tag="VEA63_15210"
     CDS             complement(2592..>2781)
                     /locus_tag="VEA63_15210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD85413.1"
                     /translation="KAGDFITFGMFLVHASLDNRTPNQLRISSDARYQRASEPIDERW
                     VGINPPGHSTAGKRGRIC"
BASE COUNT          522 a          757 c          997 g          505 t
ORIGIN      
        1 ctgcgcgggc ggcaccggcg tgaatttcca gttgtcgcgc gtgtagctgt agagatgcgt
       61 gagctcgacg gtggcgcgtt ggtagtcgag gcgcatatac gtttcctgcc gcgggctgag
      121 cgcgctgttg acgattgagc cgacggcgcc gttagcgaac gagacgattg ccatggaaac
      181 gtcctcgacc tcgatcgcgc gatcgagcgt gcgagcgatc gcgcgcacat cggtccagtc
      241 gcccatcaga tggagaaact gatccatcgc gtgaatgccc agacccatcg tcgggccgcc
      301 gagctcggtc tcccagcgtc cgcgccacgg caccgcgtag taagcggaat ctcgatacca
      361 aagagtgttg cacacgccga cgagcggacg accgaggtgg ccgttgtccg caagccgctt
      421 gaggtgcgcg gtcgaggacg cgaagcgcat ctggaaaatg caggcggtgt agcagccggt
      481 gcgttgctcg gcggcgagga tttgatcgag ctcggcgagc gagccgcaca ggggtttttc
      541 gcacaggacc caggcaccgg cttccatggc ggcgatgctc atgggggcgt gttgcgcggg
      601 cggcgtggcg acgagaacga tatcggggcg gcgctccgcg agcatcgtcg cgtaatcggt
      661 gaacgcgtga ggaatcgcgt gacgtcgggc gaattcgccg acgcgagtcg catcgatgtc
      721 gacggcggca tcgagcgtga cgcgaccttg ggtggtttcg acggcgcgca cgtgggcctc
      781 gccaatggag ccggtgccga caagcacagc gcggtaattt ttcatgagtg attcagcgta
      841 gaaataagtt gatggcgttg aagaaacagg aagggcgggg cgtcgcgcca ggtgagcccg
      901 aacatcgccg gcgctcatga ccgtgttctc gagaattgaa gggaaggcgc gcaagttagc
      961 gcgcgcgccg accgggatca cgatgaaatt cgatgaaaat tcaatcgggg tgggcggccg
     1021 ccgagaaggt gaaacccgac gttattgtcg aaccgaacag gaatagatgt tctctccagc
     1081 cgagacggga tgtcgggctc cagcggacga gagaccgaga tcgcgtagtg cgtcggtggc
     1141 ggcacgatca ggttgatcgt gctacagacg gaggacacgt ttagcgcacg ccggcgagct
     1201 gcagaatttt gttgcccgtc aaccggcgtt cgggcggcaa cgcgtgcggc gtgcgaccac
     1261 cgtagatcgc ggagagttcg gcgaaatgcg cgcgcgtggc tttcacgaat tcttcgggcg
     1321 aacgcgtgtc gccgcacaag gtcatgattt ccggcagcca gcgatgacca tagcgaacgt
     1381 ggttttgttc gtcgttgcgg tcgaaggcga gaagcgtgtc cgcttcgaag tcgttcaact
     1441 cgcgcacgcg gtccatcacc gtggctttgg tgggaaaact tcccgcctcg aattccattg
     1501 tcatcatggc gtagcgctca tgcggcggca tttgcacgag gatgttgtaa agatcgagcg
     1561 agtgctcgag cgtcatcaaa tcgacgccga gttttgggag ttgccgatag ccgaactggc
     1621 tgtggcggga ttcgtcccag agatgacgcg ccatgtcgtg atggagatcg aacggtgcgt
     1681 tgggcgtgtc gtagaaaacg gtcgcgaggt aatcgacggc gtcgcactcc atcatcagcc
     1741 agacgtaaac catcaagcgg atgacgcgcg cgtcggtctt cggattttcg agccaggagc
     1801 gcacgatcgg cgtttcgtcg ggatcgttac tgaacacgct ggagcacgtc ggaaattttc
     1861 cgcggttgca ggtgtcggga tggcggtagc tgcgctgcga ggattcccaa gcgaagccgg
     1921 cgggcagcgt gatgctggtt ttttcgccga gaaatccgcc gctcgctgcg agcgcggtca
     1981 agaggtgttc ggtccacgtg gtggtgtcgt gagtcgcgtc gccgaggaac ggcgcgattt
     2041 cacgcgcgtg gcgttcctcg tcctggatga actcttcgac gaggcgcagc gacggccagt
     2101 cggcgaggtg atgcgtggct tcgagatagt gccgataggc ggtgagtagc gcgggtttca
     2161 gcacgcgata aaagcccgcg agcgcgacgt tggcgtcggc gggcatcacc gcttcgttga
     2221 acaactggcg cagttcggcg cgcacgtttt cgtcttttcc gaagccgctg agctcgcggc
     2281 cgcgttcgcg caggaaacgc gcgtgactcg cggattccca gacgtgctgg cagagcaggt
     2341 atttcaggtc caggtcgccg acgcgataaa tgagcgtcgt ggcggtgcgg acgaactcgc
     2401 gttcgatctc gaacaacacg cgcagcaatc gagcgatgtc ctgcacggcg gccatgcggc
     2461 cggtggacga cgatgcaggc ttggaaacgg cggcggcagg aaaggaggga gcggccatga
     2521 gaagaggagg gcgtaggggc ggcccttgcg gacacccgcg ggcggcgaca agcgccgccc
     2581 ctacagggaa ctcagcaaat acgcccgcgt ttgccggcgg tggagtggcc gggcggattg
     2641 atgccgaccc agcgttcgtc gatcggctcg ctggcgcgtt ggtaacgggc gtcgctcgag
     2701 atgcggagct ggttgggcgt gcggttgtcg agcgaggcgt gcacgaggaa catcccgaac
     2761 gtgatgaagt cgccggcctt g
//
LOCUS       DASPLT010001488         1245 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_305250, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001488 DASPLT010000000
VERSION     DASPLT010001488.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1245)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1245)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1245
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_305250"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..239)
                     /locus_tag="VEA63_15215"
     CDS             complement(<1..239)
                     /locus_tag="VEA63_15215"
                     /inference="COORDINATES: protein motif:HMM:NF027966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoD-like phosphatase N-terminal
                     domain-containing protein"
                     /protein_id="HYD85414.1"
                     /translation="MPNSELLRRAFLARTGGCLALTIATSRFSLAQAPANPFRLGVAS
                     GDPLPDGVVLWTRLASDPLNGGGMPAKTVEVDWEIA"
     gene            complement(289..>1245)
                     /locus_tag="VEA63_15220"
     CDS             complement(289..>1245)
                     /locus_tag="VEA63_15220"
                     /inference="COORDINATES: protein motif:HMM:NF005495.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HYD85415.1"
                     /translation="SSGIGLATARMAARRGAKVIVAARNQEAIRRLCDEIRTAGGDAR
                     WVNADVSREEDLGRIAAEAIGHFGRIDSWVNNAGVSIYGKALDISIADARKLFETNFW
                     GVVYGSRVAMHHLRQNGGALINVGSVVSDTAVPLQSYYVASKHALKGFTDALRMETEN
                     DKIPVSVTTIKPGPIDTPYTQHAKNYLEDEPSHAPPVYDPDLVADAILHAAEHPVRDL
                     YVGGGAKGMSVLGRFAPRFADKYMEKVLAPKTHSGRPREARGDALHSHSFGGEQRGNY
                     DGTVRRTSAYTTAAMHPAAASAIGFGAAFAIGSLLRSRTRVK"
BASE COUNT          226 a          388 c          401 g          230 t
ORIGIN      
        1 gcaatctccc agtccacctc aacggtcttg gcaggcatgc cgccgccgtt cagcggatcg
       61 gaggcgagtc tggtccaaag cacgacaccg tccggcagcg gatcgcccga tgccacgcca
      121 agcctaaacg ggttagcggg ggcttgagcc agcgaaaagc gagaggtggc tatggtgagg
      181 gcgaggcacc ctccggtacg agccaggaat gcgcggcgaa gcagttcgga gttcggcatg
      241 aactccctac gataccgcgc atcccggccc cgccggaaga gaattgaact acttcacgcg
      301 agtgcggctg cgcaacagcg atccgatcgc gaatgccgcc ccgaaaccga tcgcgctcgc
      361 ggctgccggg tgcatcgccg cggtcgtata ggcactcgtg cgacggaccg tgccgtcgta
      421 gttgccgcgc tgctcgcctc cgaaagaatg cgaatgaagc gcgtcgccgc gtgcctcgcg
      481 cggtcgtccg gagtgcgtct tgggtgcgag cactttctcc atgtacttgt cggcgaagcg
      541 aggtgcgaac cgccccaaga ccgacatccc cttggcaccg ccgccgacat acaggtcgcg
      601 gaccggatgc tccgctgcat gcaggatggc atcggcaacc aagtcgggat cgtagaccgg
      661 cggtgcgtgc gagggttcgt cctcaagata gttcttcgcg tgctgggtgt acggagtgtc
      721 gatgggacct ggtttgatgg tggtgacgga gacggggatc ttgtcgttct cagtttccat
      781 cctcagcgcg tccgtgaagc ctttcagcgc gtgcttcgag gcaacatagt acgactgcag
      841 cggcacggcg gtatccgaga caacgctgcc gacgttgatc agtgcaccgc cgttctgccg
      901 cagatgatgc atcgcgactc gggagccgta cacgacgccc cagaagttcg tctcgaacag
      961 cttgcgggcg tcggcgatcg agatgtccag agctttgccg taaatggata cacccgcgtt
     1021 gttgacccag ctatcgatgc ggccgaaatg cccgatcgcc tcggccgcga ttctgccaag
     1081 atcttcttcg cgcgaaacgt cggcattgac ccatctggca tctccgccgg cagttcgaat
     1141 ttcatcgcag agccggcgaa tcgcttcttg gtttctggcg gcgacaatga ctttcgcgcc
     1201 gcggcgcgcg gccatgcggg cggttgcgag accgatcccg ctgga
//
LOCUS       DASPLT010001489         1916 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_119565, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001489 DASPLT010000000
VERSION     DASPLT010001489.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1916)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1916)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1916
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_119565"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..274)
                     /locus_tag="VEA63_15225"
     CDS             complement(<1..274)
                     /locus_tag="VEA63_15225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD85416.1"
                     /translation="MPNPLQLIVDEPIWRAHYAWSVERGGPGLTTRLFRRVFDAPAGV
                     SSLRIHVSADSRYRLWLNGVRVGHGPAKGSLGRYHFETYELAGLLKA"
     gene            527..1606
                     /locus_tag="VEA63_15230"
     CDS             527..1606
                     /locus_tag="VEA63_15230"
                     /inference="COORDINATES: protein motif:HMM:NF024770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HYD85417.1"
                     /translation="MEARATLQDIATLAGVGKATVSLALRNHPKISAATRARVQAAAE
                     QLHYRPDPALARIAAHRWRSREHPSDLTLAFITMSHPWTHDEPLIDLRLGATAQADRL
                     GYRLEHFRLDDFPGPEQLARVLFHRGIRGVIVGTIFREDFAQRFPWENFVGMGCHIGY
                     HQPPVNVVIPDFHHAVVRAWREAVNAGYRRIGLAMLRELEAVDLFDKVSAALFCQERL
                     NPELNPIPLQHFPLEDHTEFRAWMRQHQPDVVIGFNDVVHWWLREGNFRVPNEVAFIS
                     LDTQVNAEREGPMLSGMNPDYEYIGRTSVSQLDILLRTNQQGIPERPLTVHVPSVWVP
                     GETLPVRSSTEKPTRRKIARTSVGG"
     gene            complement(1638..>1916)
                     /locus_tag="VEA63_15235"
     CDS             complement(1638..>1916)
                     /locus_tag="VEA63_15235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD85418.1"
                     /translation="RRMLLQAMSEERATGYATEDAGEGLKRIVSVGTDPWQVRALSGT
                     VAFKKAEANELKVTALDFNGRPQREVPLGANATLALERATTFYLIERR"
BASE COUNT          341 a          693 c          527 g          355 t
ORIGIN      
        1 ccgccttgag cagaccggcg agttcgtagg tctcgaaatg ataccgtccg agcgaccctt
       61 tcgcggggcc gtggccgacg cgcacaccat tcaaccaaag tcgataacgg ctgtccgccg
      121 acacgtgaat ccttagactg ctcacccccg ccggagcgtc gaacacgcgc cgaaacaaac
      181 gcgttgtcag gccggggccg ccgcgctcga cgctccacgc atagtgcgcc cgccaaatcg
      241 gttcgtcgac gatgagttga agcggattcg gcacgtgcgg cgattgtgtc gcccaatcac
      301 gtctcagcgc gcacgtgctc gcaaccgatc cgcgcgtgaa cccgttccac ggaccgccgg
      361 tcaaagccgg ccgcagcttt acagcgcgct cgacgtaaca ctttctcgtt catcccgcat
      421 cggccgagcg ctcgagatcg cgctcgtcgc gttttctctc ctgtccgcgg gaatcttgcg
      481 cgtatttgca ttgaacagaa cccgtcgcac gttgaaacac cccgccatgg aagctcgcgc
      541 cacgctgcag gacatcgcca cactcgccgg cgtcgggaaa gccacggtct cgctcgcgct
      601 gcgcaatcat ccgaaaattt ccgccgccac ccgcgcgcga gtgcaagccg ccgccgagca
      661 gctccactac cgccccgacc ccgcgctcgc gcgcatcgcc gcgcaccgct ggcgcagccg
      721 cgaacatccc tccgacctca cgctcgcgtt catcacgatg agtcatccgt ggacgcacga
      781 tgaaccgctg atcgacttgc gactcggcgc gacggcgcag gccgaccggc tcggctatcg
      841 tctggagcat tttcgcctcg acgactttcc cggaccggag caactcgccc gggttttgtt
      901 tcaccgcggc attcgcggtg tgatcgtcgg caccatcttt cgcgaggact tcgcccagcg
      961 ttttccctgg gaaaacttcg tcggcatggg ctgccacatc ggctaccacc aaccgcccgt
     1021 caacgtcgtg atcccggatt ttcaccacgc cgtcgtccgc gcctggcgcg aggcggtgaa
     1081 cgccggctat cggagaattg gactcgcgat gctccgcgag ctcgaagccg ttgacctttt
     1141 cgacaaggtt tccgccgccc tcttctgcca ggaacggctc aacccggagc tcaacccgat
     1201 tccgctgcag cattttcccc tcgaggatca cacggaattt cgcgcctgga tgcgacagca
     1261 tcagccggac gtcgtcatcg gcttcaacga cgtcgtgcat tggtggctgc gcgagggaaa
     1321 ctttcgcgtt cctaatgagg tcgctttcat cagcctcgac acgcaagtga acgccgaacg
     1381 cgagggaccg atgttgtccg gcatgaatcc ggattacgaa tacatcggcc gcacctcggt
     1441 cagccagctg gacattctgc tgcgcacaaa ccagcaggga attcccgagc gcccgctgac
     1501 ggtccatgtt ccgtcagtct gggttccggg cgaaacgctt cccgttcgct catcgacgga
     1561 gaaacccacc cgccgaaaaa tcgcgcgaac gtccgtcggc ggctaaaggt ggagcccgac
     1621 gtcgctccac cccaacctca ccgccgttcg atcagataaa acgtcgtcgc ccgttcgagc
     1681 gcgagcgtgg cgttcgctcc gagcggcact tcgcgctgcg gccgtccgtt gaaatccagc
     1741 gccgtcactt tcagctcgtt cgcttcggct tttttgaacg ccaccgttcc gctcaacgcg
     1801 cgcacctgcc acggatccgt tccaaccgaa acgatccgct tcaatccctc gcccgcgtct
     1861 tcggtcgcgt agcccgtcgc gcgttcctcc gacatcgctt gcaacagcat ccgccg
//
LOCUS       DASPLT010001490         1203 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_161327, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001490 DASPLT010000000
VERSION     DASPLT010001490.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1203)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1203)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1203
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_161327"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..393)
                     /locus_tag="VEA63_15240"
     CDS             complement(<1..393)
                     /locus_tag="VEA63_15240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD85419.1"
                     /translation="MKHFLATLALLAAVAAITCVITFRLSGDPGVKAAVEQRNALEWL
                     RTDFDLDAAQFAKIKQLHESYSLVCEDHCRAIQEAQQVRRALKASGSATPDALATADR
                     RVEELRQICETAIAAHVRECAAHMSPAAG"
     gene            complement(390..707)
                     /locus_tag="VEA63_15245"
     CDS             complement(390..707)
                     /locus_tag="VEA63_15245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD85420.1"
                     /translation="MNSLEPDDTLARALADWRIRPPANPNFRPAVWQRLRQRTRETWS
                     GYVRAHASAWVLIAALTLTASGWAGLSAGKAKLAAERDAMVTAYLVELDPRVQATLRP
                     ASP"
     gene            813..>1203
                     /locus_tag="VEA63_15250"
     CDS             813..>1203
                     /locus_tag="VEA63_15250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD85421.1"
                     /translation="MKLNTLVASLLLFSSLGVVGAVAAAADKHVPAAPTESGKLIEVT
                     EKDAAWLAKARQDYPLKVCVVSDEDLGSMGDSPEYIYRVAGQPDRLVVVCCDGCSEDF
                     LSEPARFLAKIDAARKDGAKAEKGPDAG"
BASE COUNT          202 a          296 c          488 g          217 t
ORIGIN      
        1 gccggcggcg ggcgacatgt gggcggcgca ttcgcggacg tgcgcggcga tggcggtttc
       61 gcagatctgg cggagttcct cgacgcggcg atcggcggtg gcgagggcgt cgggggtggc
      121 ggagccggag gctttcaacg cacggcggac ttgctgcgct tcctgaatcg cgcggcagtg
      181 gtcttcgcaa acgagcgagt aggattcgtg gagttgtttg attttggcga actgggcggc
      241 gtcgagatcg aagtcggtgc gcaaccattc gagggcgttg cgttgctcga cggcggcctt
      301 cacgccggga tcaccgctga ggcggaacgt gatcacgcag gtgatcgcgg cgacagcggc
      361 gagcagcgcg agcgtggcga ggaagtgttt catggcgacg cgggccggag cgtggcctgg
      421 acgcgcgggt cgagttcgac gaggtaggcg gtgaccatcg cgtcgcgctc ggcggcgagt
      481 ttggccttgc cggcggagag gccggcccag ccggaggcgg tgagggtgag cgcggcgatg
      541 agaacccagg cggacgcgtg ggcgcgcacg tagccgctcc aggtttcgcg ggtgcgctgg
      601 cggaggcgtt gccagacggc gggacggaag ttggggttgg ccggcggacg gatgcgccag
      661 tcggcgaggg cgcgggcgag cgtgtcgtcg ggttcgagag agttcatgag agccttggtt
      721 gatggactat aaaccgggga acgaaaattc cctcaaactc tttgagggtt tttcgaagtt
      781 cggggtaata gctgacgaaa cccaattcac tcatgaaact taatactctc gtcgcgtcat
      841 tgttgttgtt ctccagcctt ggtgttgtcg gcgccgtcgc cgcggcggcg gataagcatg
      901 tgccggcagc tccgactgaa agcgggaagc tgattgaggt cacggagaag gacgcggcgt
      961 ggctggcgaa ggcgcgccag gattatccgt tgaaggtatg cgtcgtgtcg gacgaggacc
     1021 tcgggagcat gggtgactcg ccggagtata tttatcgggt ggcgggtcaa ccggaccggc
     1081 tcgtggttgt gtgttgcgac gggtgcagcg aggatttttt gagcgaaccg gcgcgcttcc
     1141 tggcgaagat cgatgcggcg aggaaagacg gggcgaaagc ggagaagggg ccggacgcgg
     1201 gaa
//
LOCUS       DASPLT010001491         1348 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_18332, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001491 DASPLT010000000
VERSION     DASPLT010001491.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1348)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1348)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1348
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_18332"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..191)
                     /locus_tag="VEA63_15255"
     CDS             complement(<1..191)
                     /locus_tag="VEA63_15255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD85422.1"
                     /translation="MNLPFGQTRTRIAGRHALIGPDGHVRSNVPGIHGAETVIVINPA
                     MGAKFAQLLVTFASGGRAA"
     gene            complement(344..>1348)
                     /locus_tag="VEA63_15260"
     CDS             complement(344..>1348)
                     /locus_tag="VEA63_15260"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HYD85423.1"
                     /translation="FALWGGLTPGNLDKLAALRDAGAIGFKAFMCESGIASFPRVDAK
                     TLREGMKRAAKLGMLVAVHAEDDALAAKLTVAEKAAGRTDAKAYLASRPVEVELVAIR
                     QALEIAGETECALHIVHVSSPEGIALIDEARAQRVDVTAETCPHYLLLNEKDVGRLGA
                     LAKCAPPLRDEKRRKAMWAELRAGRIQTIGSDHSPCRPEMKDSANLFAVWGGIMGIQH
                     GFELLFSEASEAEKLEKDLPVLAAVLARNVARRFRIDGRKGVLATGMDADFSLLQFGD
                     ERKIEAEDLWTRHRIGAYVGRKSRARVTHTFVRGFPVWSEGRLTNLPPPGEFLRPGPR
                     "
BASE COUNT          253 a          551 c          314 g          230 t
ORIGIN      
        1 atcgccgccc ggccgcccga cgcgaacgtc accagcaact gcgcgaactt cgctcccatc
       61 gccggattga tcacgatcac cgtctccgcg ccgtggatac cagggacatt actcctcacg
      121 tgcccatccg gcccgatcaa cgcatgccgg cccgcaatcc gtgtccgagt ctgtccgaaa
      181 ggaaggttca tgggagaaag aagctccaag ctccaagcac caagctccag agaatcctca
      241 aacttccaat ttcaaaaacg cgccgatcac gtgcggtccg cttgagactt gttttttgaa
      301 gcttctctgg agcttggatc ttggcgcttg gagcttccgc gcctcagcgc ggccccggcc
      361 tcaaaaactc acccggcggc ggcaaattcg tcagccgccc ctccgaccac accggaaacc
      421 cgcgcacaaa cgtatgcgtc acgcgcgcac gactcttccg ccccacatac gcgccgatcc
      481 gatgccgcgt ccacaagtcc tccgcctcga tcttccgctc gtcgccaaac tgcagcagcg
      541 aaaaatccgc atccatcccc gtcgccagca cgccttttcg cccgtcgatc cgaaaccgcc
      601 gcgccacatt ccgcgccagc accgccgcca gcaccggcaa atctttctcc aatttctccg
      661 cctcgctcgc ctccgaaaac aacagctcga acccgtgctg aattcccatg atcccacccc
      721 acaccgcgaa caggttcgcc gaatccttca tctccggccg gcacggcgaa tgatccgatc
      781 cgatcgtctg aatccgaccc gcgcgcaact ccgcccacat cgccttccgc cgcttctcat
      841 cgcgcaacgg cggcgcgcac ttcgccaacg cgccgagccg cccgacatct ttttcgttca
      901 acagcaaata atgcggacac gtctccgccg tcacatccac gcgctgcgcc cgcgcttcgt
      961 cgatcagcgc gatcccttcc ggactgctca catgcacgat gtgcagcgcg cactccgtct
     1021 cgcccgcgat ctcgagcgcc tggcggatcg cgacgagctc cacctccacc ggccgcgacg
     1081 cgagatacgc cttcgcatcc gtccgccccg ccgctttctc cgccaccgtc aacttcgccg
     1141 cgagcgcatc atcctccgcg tgcaccgcga ccagcatccc gagcttcgcc gcgcgcttca
     1201 tgccttcgcg caacgtcttc gcatcgacgc gcggaaagct cgcgatcccg ctctcgcaca
     1261 tgaacgcctt gaacccgatc gcgcccgcat cccgcagcgc cgcgagcttg tccaaatttc
     1321 ccggcgtcag cccgccccat agcgcgaa
//
LOCUS       DASPLT010001492         1106 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_161367, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001492 DASPLT010000000
VERSION     DASPLT010001492.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1106)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1106)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1106
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_161367"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..390)
                     /locus_tag="VEA63_15265"
     CDS             complement(<1..390)
                     /locus_tag="VEA63_15265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD85424.1"
                     /translation="MELTIAGFEIDRRKIITAVVVVLACVALALLYRQIDIEALHARA
                     QEVNGALVFVLMTVLPLVGFPVSVCHAVAGVRFGLGPGVALVALSILLQMLASYALVK
                     AVPKFFARRMEPFRKKLPKTAHGPLTVF"
     gene            636..>1106
                     /locus_tag="VEA63_15270"
     CDS             636..>1106
                     /locus_tag="VEA63_15270"
                     /inference="COORDINATES: protein motif:HMM:NF012492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HYD85425.1"
                     /translation="MLSDAFASLQKLMLPDRWQAEALAGLRAAKDVIVDAPTGAGKTY
                     VFERWAEQGNFSKRALFTVPTRALANDKFAEWRARGWRVGIMTGDLCIEPDAPVVVAT
                     LEAVQGLVTAAVSSEPRVRTAAAPFQLLVVDEYQWIGDAHRGNHYEGVLLSAPRE"
BASE COUNT          193 a          312 c          423 g          178 t
ORIGIN      
        1 gaaaacggtg agcgggccgt gcgcggtttt cggaagcttt ttgcgaaacg gttccatgcg
       61 gcgggcgaag aacttcggca cggctttgac cagcgcgtag ctggcgagca tttggaggag
      121 aatcgataac gcgacgagtg cgacgcccgg gccgaggccg aagcgcacgc cggcgaccgc
      181 gtggcacacg ctgacgggaa atccgacgag cggcaggacc gtcatcaaca cgaagacgag
      241 cgcaccgttc acttcttgcg cgcgagcgtg cagcgcctcg atgtcgatct gacggtagag
      301 caacgcgagc gcgacgcagg cgaggacaac gacgacggcg gtgatgattt tacgacggtc
      361 gatctcgaag cccgcgatgg tgagttccat gcgatgcaga ccgcgggcgg atgcggaggt
      421 tcgcaggtgg agcccgacgt cccccttggg ctgctgcaag atagggcgag gcgtctcgcc
      481 gagccggtta cgcgcccccg gctcggcggg gacgcctcgc cctaccaaag cgccgaagtg
      541 cgacgaggac gtcgggctcc acccatgcgg cgaatcgctg gtaaatttct gttcgccggt
      601 ttgaccggca acgcaccggc cgccatcctg cgcgcgtgct gtccgacgcg tttgcgagtt
      661 tgcaaaagct gatgctgccg gatcgctggc aggcggaagc gctggcgggc ctgcgcgcgg
      721 cgaaggatgt gatcgtcgac gcgccgacgg gcgcgggaaa gacgtatgtg ttcgagcgtt
      781 gggcggagca gggaaatttc tcgaagcgcg cgctcttcac ggtgccgacg cgtgcgctgg
      841 cgaacgacaa gttcgcggag tggcgggcgc gcgggtggcg cgtgggaatc atgaccggcg
      901 atttgtgcat cgagccggac gcgccggtgg tggtggcgac gttggaagcg gttcaagggc
      961 tggtgacggc ggcggtgtcg tcggagccgc gagtgcggac ggcggccgcg ccgtttcaac
     1021 tgctggtcgt ggacgagtat cagtggattg gagacgcgca tcgcgggaat cactacgaag
     1081 gcgtgttgtt gtcggccccg cgggag
//
LOCUS       DASPLT010001493         1795 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_78664, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001493 DASPLT010000000
VERSION     DASPLT010001493.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1795)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1795)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1795
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_78664"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..935)
                     /locus_tag="VEA63_15275"
     CDS             complement(<1..935)
                     /locus_tag="VEA63_15275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD
                     domain-containing protein"
                     /protein_id="HYD85426.1"
                     /translation="MLFFMTMLRWFNPSFAPERRLRLFGMFAACLLVFPAALPAAAPA
                     QPSHVQASLVAAESSIQPGRPFTVALRLVHESHWHTYWLNPGTGLATTIAWTLPEGFT
                     AGEIQWPAPIVLKDSSGAIVGNGYEGEMFLLVTITPPASLSAGANVELKADAEWLMCS
                     DVCIPGNADLTLSLPVSSEPPQPNSEWSTKIAATAAALPRARAEWTVNATRDAEQLTL
                     TLTPASGVAHTPERLHFFSDDNLVAYDLPQDVKADGRGGYVLTAPISPDAPENPTHAH
                     GVLTSENGWLSGDASRGLRVDVALAAATAATGAATP"
     gene            complement(1056..>1795)
                     /locus_tag="VEA63_15280"
     CDS             complement(1056..>1795)
                     /locus_tag="VEA63_15280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD85427.1"
                     /translation="FMPHTVTSVEPPWRSGLRSARANLLPGLVLQAAALALVFAYYRH
                     EPSRAAIGEIASAHERIGVVFSMLSTGFFGGLLPVIYLRSRAATRSHCSLAQGAVFTA
                     FWTYKGLEVDVFYRLLARFVGEGNDVATIAVKTAIDQFVYCPLLAVPLTVVVYEWTLS
                     GFNASGVTADMRAPGWLRRRVLPVLISNVGVWLPTACIIYSLPTPLQLPLQNLVLCFF
                     TLLVATQTRRAGDAARAPRIERARERR"
BASE COUNT          344 a          591 c          576 g          284 t
ORIGIN      
        1 ggagtggccg cgcctgttgc cgccgtcgcc gcagcgagcg ccacatccac gcgcaatccc
       61 cgcgacgcat cgccgctcag ccaaccgttt tccgacgtga gcaccccgtg ggcgtgcgtg
      121 ggattttccg gcgcgtcggg cgagatcggc gccgtcaaaa cgtagccacc gcgaccgtcg
      181 gccttcacgt cctgcggcag atcgtaggcg acgagattgt cgtctgaaaa aaaatgcagc
      241 cgctcgggcg tgtgcgcgac gccgctcgcc ggcgtcagcg tgagcgttaa ctgttccgca
      301 tcgcgagtcg cgttaacggt ccattccgct cgtgctcgtg gcaacgccgc ggccgtcgcc
      361 gcgattttcg tggaccattc ggagttcggt tgcggcggct cgctggaaac cggcagcgac
      421 aacgtcaggt ccgcgttgcc cgggatgcag acgtcgctgc acatcagcca ttccgcgtcg
      481 gctttcagtt ccacgttggc gcccgcggag agcgaggccg gcggcgtgat cgtcaccaga
      541 agaaacattt cgccctcgta gccgttgccc acgatcgcgc ccgagctgtc cttgagcacg
      601 atcggcgcgg gccactgaat ctcgcccgcg gtgaatcctt ccggcagcgt ccaagcgatc
      661 gttgtcgcga ggccggtgcc tggattcagc caatacgtgt gccagtgcga ttcgtgcacc
      721 aagcgcagcg cgaccgtgaa gggccgcccc ggctggatcg agctctcggc cgcgaccaac
      781 gatgcctgca catgagacgg ttgcgcgggt gccgccgctg gaagtgcggc cgggaaaacg
      841 agcagacagg ccgcgaacat tccgaacagg cgcaacctgc gttccggcgc gaaacttggg
      901 ttgaaccagc ggagcatcgt catgaagaaa agcatgcgga gtatgacgtg agaggcaaag
      961 actcggtcgc aaattccgcg agaccttcca cgtaacgatt tagtccatcc gcctcgctct
     1021 cgttttaccg gtgagaccga cagcgtgacg ctggtttatc gccgctctct cgcccgttcg
     1081 atccgcggcg cgcgcgccgc atctccggcg cgtcgcgttt gcgtcgccac gagcagcgtg
     1141 aaaaagcaaa gcacgagatt ttgcagcgga agctgcaaag gcgtcggcag cgagtaaatg
     1201 atgcacgcgg tcggcagcca tacgccgaca ttcgagatca acaccggcag cacgcgccgc
     1261 cgcagccaac ccggcgcgcg catgtcggcg gtcacgccgg aggcattgaa gcccgagagc
     1321 gtccattcgt agaccacgac cgtcagcggc accgcgagca gcggacaata cacgaactgg
     1381 tcgatggcgg tttttaccgc gatggtcgcg acatcgttgc cctcgccgac gaaccgcgcg
     1441 agcagtcggt agaacacgtc gacttcgagc cccttgtaag tccagaacgc ggtgaacacc
     1501 gctccctgtg cgaggctgca atgcgagcgc gtcgccgcgc gcgaacgcag ataaatcacc
     1561 ggcagcagcc cgccgaaaaa cccggtcgag agcatcgaga acaccacgcc gatgcgctcg
     1621 tgagcgctgg cgatttcgcc gatggccgcg cgcgacggct cgtgccggta gtaggcaaac
     1681 acgagcgcga gcgccgcggc ttgaagcacc aagccaggta aaagatttgc ccgcgcgctc
     1741 cgcagacccg accgccaagg cggttcaacg gacgtgacgg tgtgcggcat gaacg
//
LOCUS       DASPLT010001494         1924 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_161415, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001494 DASPLT010000000
VERSION     DASPLT010001494.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1924)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1924)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1924
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_161415"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1788)
                     /locus_tag="VEA63_15285"
     CDS             complement(<1..1788)
                     /locus_tag="VEA63_15285"
                     /inference="COORDINATES: protein motif:HMM:NF019552.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heparinase II/III family protein"
                     /protein_id="HYD85428.1"
                     /translation="MLSRSLLNSLRCAIAGLAAVAAVHAEYRIDSKTVPTAVIQPEAA
                     FEVHANHPRLYFRDTDLPAIRRRIRAEFREPWETMRAQIERTVLSQPAEKFAHGQYLK
                     GWQSARSVVFVAAVTGEENYLAWSRQWMEALVAEPPGNTDDELRGRLQCLAIAYDWLY
                     PWLNDAEKAHAQVAIVAHLEKAWRFASHHLNFVSGHSRWGNFVLAAGLLAIANERPEL
                     REKLLTVRRNWTEGYFPTQGWIASEGGYHMGWSYSSAYLTGEIHCVWSSATNDCVYFP
                     WQAKLPLMWIYGRQGDGFYPNTGDAYTLSGDFTSLRTLLVTAAGVLKDPHARWLLPAK
                     GDHFVEILYGDADVVARAPDEASAPLPLSRNFGPAGVVLARDRWDEASTVLQFRSVPF
                     YSANHHHRDENSFTLHYRGPLAIDSGLYDEGGSGAQRGGYGGAHWLNYFTRTVAHNAI
                     VVFDPTQVMTYGDTPISNDGGQPFRTEPSQLTDLLPGGAAHLDGITHYRDTPEFTVAS
                     GDATKAYDPARVRLAQRDIVYLRDSGRPHPVVVVFDRVESAKPELKKRFLLHTVNEPT
                     VQNRLMVTENLGGRLSSLTLLPEDAKLALV"
BASE COUNT          368 a          654 c          564 g          338 t
ORIGIN      
        1 cacaagcgca agcttggcgt cctccggcag cagcgtgagc gacgataacc gaccgccgag
       61 attctccgtc accatcaaac ggttctgcac cgtcggctcg ttcaccgtgt gcagcagaaa
      121 acgttttttc aattccggct tcgcgctctc gacgcgatcg aacacgacca ccactggatg
      181 cggccgcccg ctgtcgcgca gatacacgat gtcgcgctgc gccagacgca cgcgcgccgg
      241 atcgtaagcc ttcgttgcat cgccggacgc gaccgtaaac tccggcgtgt cgcgatagtg
      301 cgtgatgccg tcgagatgcg ccgcgccgcc gggcagcaga tccgtcaact gcgacggctc
      361 cgtgcgaaac ggctggccgc cgtcgttcga gatcggcgtg tcgccatagg tcatgacctg
      421 cgtcgggtcg aaaaccacga tcgcgttatg cgccaccgtg cgcgtgaagt agttaagcca
      481 gtgcgccccg ccgtagccgc cgcgctgcgc gccgctgccg ccttcatcat agagccccga
      541 atcgatcgcc agcggtccgc gatagtgcaa cgtgaacgag ttctcgtcgc gatggtgatg
      601 gttcgccgaa taaaacggca ccgaacgaaa ctgcagcacg gtgctcgctt catcccagcg
      661 atcgcgcgcg agaacgacgc ccgccgggcc gaaattccgg ctgagcggca gcggagcgct
      721 cgcttcgtcc ggcgcacgtg ccaccacatc ggcatcgcca tacaggatct cgacgaagtg
      781 atcgcctttc gccggcagga gccagcgcgc gtgcggatct ttcagcacgc ccgccgcggt
      841 caccagcagc gtgcgcagcg acgtgaaatc gcccgacagc gtatatgcgt cgccggtgtt
      901 cggataaaag ccgtcgccct gccgcccgta gatccacatc agcggcagct tcgcctgcca
      961 cggaaaataa acgcagtcgt tggtcgcgct cgaccacacg caatgaatct cgccggtgag
     1021 gtaggcggag ctgtaactcc agcccatgtg ataaccgcct tccgaagcga tccaaccctg
     1081 cgttggaaaa taaccctccg tccagtttcg ccgcaccgtc agcagttttt ctctcaattc
     1141 cgggcgctcg ttcgcaatcg cgagcagtcc cgccgccagc acaaaattac cccaccgcga
     1201 atgcccgctg acgaagttca ggtgatggct cgcgaaccgc cacgcctttt cgaggtgcgc
     1261 aacgatcgcc acctgcgcgt gcgccttctc cgcgtcattc aaccatggat acagccaatc
     1321 gtaagcgatc gccagacact gcaaccggcc gcgcaactcg tcgtcggtgt tccccggcgg
     1381 ctccgcgacg agcgcctcca tccattggcg ggaccaggcg agatagtttt cctcgcccgt
     1441 caccgcggcg acaaacacca cgctccgcgc cgactgccag cctttcaaat actgcccgtg
     1501 cgcgaatttt tcggccggtt gcgacaagac ggtgcgctcg atctgcgcgc gcatcgtttc
     1561 ccacggctcg cgaaactccg cccggatgcg ccgccggatg gccggcagat cggtgtcacg
     1621 gaaatacaac cgcggatggt tcgcgtgaac ctcaaacgcc gcctccggct gaatcaccgc
     1681 tgtcggcacc gtttttgaat cgatgcgata ctcagcgtga accgccgcga cggcggccaa
     1741 gcccgcgatc gcgcaacgaa gggaattgag aagtgatcga gataacatgc taaagggagt
     1801 ttacggcgtg cggcgaagcg gcatgagcat cgcgtgcaac tcggcgacga gctgcgcgtg
     1861 ctccggcgca cgcaccaggt tttgcgtttc tttcgggtcg ttcgtttcgt cgaagagctg
     1921 cgcg
//
LOCUS       DASPLT010001495         1416 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_78671, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001495 DASPLT010000000
VERSION     DASPLT010001495.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1416)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1416)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1416
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_78671"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..311)
                     /locus_tag="VEA63_15290"
     CDS             complement(<1..311)
                     /locus_tag="VEA63_15290"
                     /inference="COORDINATES: protein motif:HMM:NF012645.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit B"
                     /protein_id="HYD85429.1"
                     /translation="MTLPLFLAAAVEAHAAEPGMLERFGVEPKYIIMQMISFLIVLGV
                     LYKFGIKPVTATMEERNRKIESGIKHAEEMQVKLAATQQESAALIKKSQLDASRIVDD
                     AR"
     gene            complement(380..589)
                     /locus_tag="VEA63_15295"
     CDS             complement(380..589)
                     /locus_tag="VEA63_15295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HYD85430.1"
                     /translation="MPILIAELTGNIASAFGLLGAALGVGLIGFKAAEAVGRNPAASG
                     KVLVQAIIGMALAEGLGILALFLAS"
     gene            complement(664..>1416)
                     /locus_tag="VEA63_15300"
     CDS             complement(664..>1416)
                     /locus_tag="VEA63_15300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HYD85431.1"
                     /translation="AMATGWAMSLLLVMLVLWLIGKPSVVPSKGQAVIESLMEALRGV
                     LEPIVGARAFPAAFPILITFFIFILFHNWMGLIPGVGTMGWGHDVDGHFHLTRPFIRP
                     HNADFNGTIALALISFGAWAIIVFKFAGLKTIAWDLFGNKAEKNETPGWLYPILSLVF
                     LLVGFIEVFSIAIRPFTLSVRLFGNVFGGENLLHGTGFIFVFYFMELLVGVIQALVFT
                     LLTAVYIGLLCNHEGGDHAHGDERGHAAETHH"
BASE COUNT          321 a          383 c          465 g          247 t
ORIGIN      
        1 cgggcgtcgt cgacgatgcg agaggcgtcg agctgggact tcttgatcag cgcggcgctt
       61 tcctgctgcg tggccgcgag cttcacctgc atttcttccg cgtgcttgat gccggattcg
      121 atcttccggt tgcgctcttc catcgtcgcg gtgacgggct taatgccgaa tttgtagagc
      181 acgccgagga cgatcagaaa actgatcatc tgcatgatga tgtatttcgg ctcgacgccg
      241 aaacgttcga gcatgccggg ctcggcagcg tgggcttcga cagcggcggc gaggaagaga
      301 ggcagcgtca tggcaggcgg aaaaggcgaa agggaaatgg cagcgcgagg agctcgcgct
      361 gccggagaaa cgagaacgtt tagctggcga ggaacagcgc gagaatgccg agaccttcgg
      421 cgagcgccat accgatgatg gcttggacga gaaccttgcc ggaagcggcg ggattgcgac
      481 cgacggcttc ggcggctttg aatccgatca aaccgacgcc gagggcggcg ccgagaagac
      541 cgaacgcgct ggcgatgttg ccggtgagtt cagcgatgag gatgggcatg gtggtatctg
      601 ttttgtttgt gggtttccgc cgtgcgcacg cttggcacgc tcccggcggg aaagagatgc
      661 gaatcagtgg tgcgtttcag ccgcgtgacc gcgttcgtcg ccgtgcgcgt ggtcgccgcc
      721 ttcgtgatta cagagcagtc cgatataaac cgcggtcaac aacgtgaaga ccagcgcttg
      781 aatcacgccg acgagcagct ccatgaagta gaacacgaaa atgaagccgg tgccgtgcaa
      841 cagattctcg ccgccgaaga cgttgccgaa aagacgaacg gagagcgtga aggggcggat
      901 tgcgatcgag aagacttcga tgaaaccgac gagcaggaaa acgagtgaga gaatgggata
      961 cagccaaccg ggcgtctcgt ttttctcggc cttgttgccg aagagatccc aagcaatggt
     1021 tttcagcccg gcgaatttga agacgatgat cgcccaagca ccgaacgaga tcagcgcgag
     1081 cgcgatcgtg ccgttgaagt cagcattgtg cggacggatg aacggacgcg tcagatgaaa
     1141 gtggccgtcg acgtcatgcc cccatcccat ggtgccgaca cccgggatga gccccatcca
     1201 gttatgaaag aggatgaaaa taaaaaacgt gatcaggatg ggaaacgcgg cgggaaacgc
     1261 ccgcgcgccg acgatcggtt cgagcacgcc gcgcaatgct tccatcaagg actcgatcac
     1321 cgcctggcct tttgacggca ccacggaagg tttgccgatc agccacagca ccagcatgac
     1381 gagcagcagc gacatcgccc agccggtggc catcgc
//
LOCUS       DASPLT010001496         1370 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_161457, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001496 DASPLT010000000
VERSION     DASPLT010001496.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1370)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1370)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1370
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_161457"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(247..>1370)
                     /locus_tag="VEA63_15305"
     CDS             complement(247..>1370)
                     /locus_tag="VEA63_15305"
                     /inference="COORDINATES: protein motif:HMM:NF013909.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HYD85432.1"
                     /translation="GRLRVQEFEHGDDPDKVVWAVWSPTGEGRRFRHELTAVPGKLLA
                     AERMPLGEELPLSGAATQRSDGAVDVEVTESPVYLVFQRNPSMTLSSPSISSAARPLR
                     AHELNAWLRALHPVPEPSVDRVIAGDPQEEVKGIAVMWTPTWAALREALTQGCNVLVA
                     HEPTFFSHLDLDAFEGEKSELSKAGVKAMSATRDAKLKWIQDNGMVVIRCHDVLDLIP
                     GGVADSLARRLGFSEADVLVNQPYYRVVKVVPAMRAIDLAQRMAQVFGTIGQPGVAFY
                     GDPERKVEKLGIGTGYACEPWTFVELGADMCVTIDDRIKTWIETEWADDAGYPMIVIH
                     HGTSEEWGVHTLHGTLAKAFPDLPVKLIAQGFRARWVSP"
BASE COUNT          263 a          530 c          332 g          245 t
ORIGIN      
        1 tctccatcga ccccgccgcg ccgctccacc tcccgggcgc cgccttcttc atcgccgccc
       61 tcaccctcgc cgccgcctgc ctcctcaccg cgcgcatcac gcgcccctaa cctttgtcat
      121 ctattagatg acaaacccac ccccacgtaa cggggggcca tgctcgggac gtttgtcatc
      181 tattgtatga caaacgcccg cgtcgcagaa ctttcactac acgccacgcg tgcttccgaa
      241 cctcgactac ggcgaaaccc atcgcgcgcg aaaaccctgc gcgatcaact tcaccggcag
      301 atccggaaac gccttcgcga gcgttccgtg cagcgtgtgc acgccccact cttcgctcgt
      361 gccgtgatga atcacgatca tcggataacc cgcatcgtcc gcccactccg tttcaatcca
      421 cgttttgatc cgatcgtcga tcgtcacgca catgtctgcg ccaagttcca caaacgtcca
      481 cggctcgcac gcgtaaccag tgccgattcc gagtttctcc accttccgct ccggatcgcc
      541 ataaaacgcg acgcccggct ggccgatcgt cccaaacacc tgcgccatcc gctgcgcgag
      601 atcgatcgcg cgcatcgccg gcaccacttt caccacccga taatacggtt ggttcaccag
      661 cacatccgct tcgctgaatc ccaaacgcct cgccagcgaa tcggccacgc cgccgggaat
      721 cagatccagc acgtcatggc agcgaatcac caccatgccg ttgtcctgaa tccatttcag
      781 cttcgcgtcg cgcgtcgcac tcatcgcttt cacccccgcc ttcgacaact cgctcttctc
      841 tccctcgaac gcatccagat cgagatgact gaaaaacgtc ggctcgtgcg ccaccaacac
      901 attgcacccc tgcgtcagcg cctcgcgcaa cgccgcccag gtcggcgtcc acatcaccgc
      961 aatccctttc acttcctcct gcggatcgcc cgcgatcacg cgatcgaccg agggttccgg
     1021 cactggatga agcgcacgca gccaggcatt gagttcgtgc gcgcgaagcg gacgcgcggc
     1081 agatgagatc gagggcgaag acagggtcat ggagggatta cgttgaaaaa ccaaatagac
     1141 aggactctcc gtcacttcga catcaaccgc gccatcgctg cgctgagtcg ctgcgccgga
     1201 taacggcaac tcctcgccga gcggcatccg ctcggccgcc aggagtttcc cgggcaccgc
     1261 ggtcagctcg tgccgaaatc gccggccctc gcccgtcggc gaccacaccg cccacacgac
     1321 tttgtccgga tcgtcgccgt gctcgaactc ctgcacgcgc aaccggccgg
//
LOCUS       DASPLT010001497         5087 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_161522, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001497 DASPLT010000000
VERSION     DASPLT010001497.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 5087)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5087)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5087
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_161522"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..877)
                     /locus_tag="VEA63_15310"
     CDS             complement(<1..877)
                     /locus_tag="VEA63_15310"
                     /inference="COORDINATES: protein motif:HMM:NF016430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HYD85433.1"
                     /translation="MKSREPMAERVEGAPDLRYLERFCGARAFDANAPATSEIEALFS
                     RIAALATVASNARQSGDESTAMEHFRNAIALASAALEINSFAEGDLARRRVAGVMMCL
                     ALSCGEADAARSHMTGLERLSGPTAEIDGWAELRDIELWPDAWLVAAIRRDPPDGESL
                     EALAARYWRPLFGRCEMLTLHREKAADLAQEAWRRVLRNRHALKPGGNFPAYLHRVAT
                     NIWRDGHRASRRAGNMAEDRLASLNAPISLEDGTAFSLGDAIPDLRSMDAEQQRSLMA
                     DIDHALGLLAPHLREV"
     gene            complement(1490..4102)
                     /gene="clpB"
                     /locus_tag="VEA63_15315"
     CDS             complement(1490..4102)
                     /gene="clpB"
                     /locus_tag="VEA63_15315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HYD85434.1"
                     /translation="MDFAKLTQMSRQAVTDAQNIARRLNHNEVDTWHLLSALLAQENG
                     IVPGLLEKLSIGPGAMQLAVERELERLPKVTGSVDTSKIYVTQAVNEVLTRAENEAEK
                     LKDEYVSVEHLLLGLIDVAKPDALKKLLKSFELDRAKVLGALKQVRGAQRVTTDNPEA
                     TYAALEKYGIDLVAQARKGKMDPVIGRDEEIRRTIRILSRKTKNNPVLIGEPGVGKTA
                     IVEGLAQRILRGDVPEGLKDKTIFSLDMGALVAGAKYRGEFEERLKAVLNEVKQSEGR
                     IILFIDELHLIVGAGKTDGAMDAGNLLKPMLARGELHCIGATTLDEYRKHIEKDAALE
                     RRFQPVVVDQPTVEDAISILRGLRERFELHHGVKIQDNALVSAVVLSHRYISDRFLPD
                     KAIDLVDEACAMIRTEMDSMPQELDELTRRVLRLEIEETALAKEKDEASKRRLETLRK
                     ELAEAREQANAIRLTWEKEKASIQKTRKLREEIEALKLEMQKAERAYDLNKVAELRHG
                     RIPQMEAELKRLEQKGQEQTTLFKEEVSQEEIAEIVSKWTGVPVTRLVEGEKEKLLRL
                     EEVLHERVVGQNEAVTLVTEAILRARSGIKDPRRPVGSFLFLGPTGVGKTELAKTLAE
                     TLFDTEAAMVRIDMSEYMEKHSVARMIGAPPGYVGYDEGGQLTEAVRRKPYAVILFDE
                     IEKAHPDVFNVLLQVLDDGRITDSQGRTVDFKNTVIIMTSNIGSRFLLEGVSGDSIPE
                     AVRESVMAELRHSFRPEFLNRIDETILFKPLSLEEITKIVDLLLADLNQRLAERRVTV
                     KLDAKAKEWTAEKGYDPVFGARPLKRFLQRHIETKLARALIAGEVAEGTEVTFTVERD
                     DLAMAGRAAAARKS"
     gene            complement(4425..>5087)
                     /locus_tag="VEA63_15320"
     CDS             complement(4425..>5087)
                     /locus_tag="VEA63_15320"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HYD85435.1"
                     /translation="SSDDLHVFTFGGDGDGFSIGGNHLVHTARKNVNLTYVIMDNFVY
                     GLTKKQTSPTSPLGFKSKTDPTGAMDQPINPMRTLLNSGATFVARSHATQVNHMTDML
                     LRAAKHPGFSVVEILSECVEFFPGAFDAGNPRKGGAFKLIEEKKNDGTPEDALRHDPS
                     DEVAAFKLASEPWPGPFGVFYEAKRPTKNQLEAGLIAKAKERTKGASDLALLQASFAK
                     LR"
BASE COUNT          942 a         1713 c         1388 g         1044 t
ORIGIN      
        1 gcacctcgcg aagatgtggc gcgagcaggc ccagcgcgtg atcgatgtcc gccatgagcg
       61 agcgctgctg ctccgcatcc atcgacctca ggtccgggat cgcatccccc agcgagaagg
      121 ccgttccatc ctcgagcgat atcggcgcat tgagcgacgc gagccgatct tcggccatgt
      181 ttccggcccg gcgtgacgcg cgatgcccgt cgcgccagat attcgtcgca accctgtgca
      241 gatatgccgg aaaattcccg cccggcttga gcgcgtgccg attgcgcagc acgcgtcgcc
      301 acgcctcctg tgcgaggtca gccgccttct cccgatgaag cgtgagcatt tcgcaacgtc
      361 cgaaaagcgg ccgccaataa cgcgcagcca gagcttcgag cgactcgccg tcgggaggat
      421 cgcgccgaat cgccgcgacc agccaggcgt ccggccacaa ttcgatgtcg cgcaactcgg
      481 cccatccgtc gatttctgca gtcggtccgc tgagtctttc tagaccggtc atgtggcttc
      541 tcgcggcgtc ggcctcacca caactcagcg caagacacat catcacgccc gcgacgcgcc
      601 gccgcgcgag atcgccctcg gcgaaggaat taatttcaag ggcggcggac gccagggcga
      661 ttgcattgcg aaagtgctcc atcgcagtcg attcgtcacc gctctggcga gcgttcgagg
      721 ccacggtcgc gagcgcggcg attcgcgaga aaagcgcttc gatctccgat gtcgcgggag
      781 cattggcatc gaacgcccgc gcaccgcaaa atcgctcgag ataccgaaga tccggcgcgc
      841 cctccacccg ctcggccatc ggttcgcggc ttttcacgac ggaaagcacc ggcctgaagc
      901 gacgcgtggt ggaagtaatt cgccgcgacg acctgagacc gattgcggca gaaaccgatt
      961 ccgatccaag ctcgggaagg agtgtgcctg acggcgaaac aagggcagca tgggaaggca
     1021 taacgcggag tgcttcaccc gaacggcaat ggcactcctt cgcatacgtc ccgacgcgac
     1081 gacctgaaaa tgaaatctaa aatatttgag cgcgaagcgc ccattcaccg gttcccataa
     1141 caacaaagct ttggttcgca tcgcgatcac gggaaagatc attgaatgaa gtgagtccaa
     1201 tccgctcacg atccttaccg agatgaccgg aggtttcgac attaacattc tctcgagcct
     1261 cagccggact cacgcagcag gaaccgttcg cccgtcgaaa aagcaaaaca cggaagatgg
     1321 agcccgacgt cctcgtcgag cttgacgacg ttacgcacgc aaacagcccc accaggacat
     1381 caatcttccc cttcggtcat tcgtgtctct tcgtgtccgt tcgtggttaa actggactgt
     1441 tccggctctg ccatgctgcc acgtcgcgat tctttccgga tcgccgacat caacttttcc
     1501 gcgctgccgc tgctctcccc gccatcgcga ggtcatcgcg ttccacggta aacgtcacct
     1561 cggtgccctc ggcgacttcg cccgcaatca acgcgcgcgc gagcttcgtc tcgatgtggc
     1621 gctggagaaa acgtttcagc ggtcgcgcgc cgaacaccgg gtcgtagccc ttttcagccg
     1681 tccactcctt ggccttcgca tcgagcttca cggtcacacg acgctccgcc aggcgctggt
     1741 tgagatcggc cagcaacaag tccacgattt tggtgatttc ttcgagcgag agcggcttga
     1801 agagaatcgt ttcatcgatg cgattgagaa actccggccg gaacgaatgc cgcagctccg
     1861 ccatcacgct ctcgcgcacc gcttccggaa tcgaatcgcc cgacacaccc tcgagcagaa
     1921 agcgcgagcc gatgttcgac gtcatgatga tgaccgtgtt tttgaaatcg accgtgcggc
     1981 cttgcgagtc ggtgatccga ccatcgtcga gcacttgtag gagaacgttg aatacatccg
     2041 ggtgcgcctt ctcgatttcg tcgaagagaa tcaccgcata cggcttccgt cgcaccgctt
     2101 cggtcaactg cccgccttcg tcgtaaccga catagcccgg aggcgcgccg atcatccgcg
     2161 cgacgctgtg cttttccatg tattcggaca tgtcgatccg caccatcgcc gcttcggtat
     2221 cgaacaacgt ttccgccagc gttttcgcca gctccgtctt gccgacgccc gtcggcccca
     2281 agaatagaaa acttcccacc ggccggcgcg gatccttgat gccgctccgc gcacgcaaga
     2341 tcgcctcggt caccagcgtc accgcctcgt tctgaccaac cacgcgctcg tgcaacactt
     2401 cctcgaggcg cagcagcttt tccttttcgc cttcgacgag ccgcgtgacc ggcacgcccg
     2461 tccacttcga cacgatctcc gcgatctctt cctgcgaaac ctcctctttg aacaaggtcg
     2521 tttgctcctg ccctttctgc tcgagccgct tcaactccgc ttccatctgc ggaatccgcc
     2581 catgtcgcag ctcggcaacc ttgttgaggt cgtaagcgcg ttccgctttc tgcatctcga
     2641 gtttcaacgc ctcgatctcc tcacgcagtt tgcgcgtctt ttgaatcgac gccttctcct
     2701 tttcccacgt gagccgaatc gcgttggcct gctcgcgggc ctccgcgagc tctttgcgca
     2761 acgtttccag ccggcgcttg ctcgcttcgt ctttttcctt cgcgagcgcg gtctcctcga
     2821 tctcgagccg caacacgcgc cgcgtgagtt cgtccaactc ctgcggcatc gaatccattt
     2881 cggtgcggat catcgcgcac gcttcgtcca caagatcgat cgccttgtca ggaagaaaac
     2941 ggtcgctgat gtagcgatgc gacaacacca ccgcgctcac gagtgcgttg tcctgaatct
     3001 tcacgccgtg gtgcagttcg aaccgctcgc gcaacccgcg cagaatcgaa atcgcgtctt
     3061 ccaccgtcgg ctgatcgacg acgaccggct gaaagcgccg ctccagcgcc gcatcctttt
     3121 cgatgtgctt gcgatactcg tccagcgtcg tcgccccgat gcagtgcaac tcgccgcgcg
     3181 ccagcatcgg cttgaggaga ttgccggcat ccatcgcgcc gtcggttttc ccggcaccca
     3241 cgatcaagtg cagttcgtcg atgaacaaaa tgatccgccc ttcgctctgc ttcacttcgt
     3301 tgagcacggc cttcagccgc tcctcgaact cgccgcgata ttttgcaccc gccacgagcg
     3361 cgcccatatc gagcgaaaaa atcgtcttgt ctttgagccc ctccggcacg tcgccgcgca
     3421 ggatgcgttg cgcgagtccc tcgacgatcg cggtcttgcc gacgcccggc tcgccgatga
     3481 ggacgggatt gtttttcgtt ttgcgtgaga ggatacggat cgtgcgacgg atttcttcgt
     3541 cgcggccgat gacgggatcc atcttcccct tccgggcctg agcgacgaga tcgatgccgt
     3601 atttctcgag cgccgcgtaa gtcgcttccg gattgtccgt cgtcacccgc tgcgcgccgc
     3661 gcacttgctt caacgcaccg agcacttttg cgcggtcgag ctcgaagctc ttcaacagtt
     3721 ttttcaacgc gtcgggtttc gccacgtcaa tcagcccgag cagcaggtgc tccaccgaga
     3781 catactcgtc tttcagtttt tccgcctcgt tctccgcgcg ggtcagcacc tcattcaccg
     3841 cttgcgtgac gtaaatcttc gatgtgtcga cgctaccggt gactttcgga agacgctcga
     3901 gctcgcgttc caccgcgagc tgcatggctc caggcccgat cgacaatttt tcgagcaggc
     3961 cgggcacgat accattctcc tgcgcgagca acgcagaaag caggtgccac gtgtcgactt
     4021 cgttgtggtt gagtcgacgg gcgatgtttt gcgcgtcggt gacggcctga cgcgacatct
     4081 gggtgagctt cgcgaaatcc atggtgaaag cctgttcgca aaatccgcgc cagcaactcg
     4141 cgcgcgcagc tccgtcggaa ggaggcgaaa accccggtgg acgcgtgcca aaccgtatca
     4201 ctcaccgtgc cacgaacgtg acaagctgac taatggagcc gcggcgccct cgccgcgcgg
     4261 cacgcgacac tgtgattcgc atgacctctc aaccaagaga caacctgacc gattcgagat
     4321 cgtgtagggc ggggtctccc gaccccgccg cctccgctgc aacggcgggg tgcggagacc
     4381 ccgccctaca aagcgatgtc ggacaaccgc gccctattct tcgctcaccg caacttcgcg
     4441 aacgacgcct gcaacagggc caaatccgac gcgcctttgg tccgctcctt cgctttcgcg
     4501 atcaagccag cctcgagctg attcttcgtc ggccgcttgg cctcgtagaa cacgccgaac
     4561 ggccccggcc acggctcgct cgcgagcttg aacgccgcga cttcgtcgct cggatcatgg
     4621 cgcaacgcat cttccggcgt gccgtcgttt ttcttttcct caatcagctt gaacgccccg
     4681 cctttgcgcg ggttgcccgc atcgaatgcg ccggggaaaa attccacgca ctccgacaaa
     4741 atttcgacca ccgaaaaacc cggatgcttc gccgcacgca gcagcatgtc cgtcatgtgg
     4801 ttcacctgcg tcgcatgact gcgcgcgaca aacgtcgcgc cgctgttcag caacgtgcgc
     4861 atcggattga tcggttggtc catcgctccc gtcggatcgg tcttcgattt gaaaccgagt
     4921 ggcgacgttg gcgacgtctg cttcttcgtg agaccgtaga cgaagttatc catgatcacg
     4981 taagtcaggt tcacgttctt gcgcgccgtg tgcacgagat gatttccgcc gatggaaaat
     5041 ccgtcgccgt cgccgccaaa cgtgaacacg tgcaggtcgt cgctgct
//
LOCUS       DASPLT010001498         1503 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_202834, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001498 DASPLT010000000
VERSION     DASPLT010001498.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1503)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1503)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1503
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_202834"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1503)
                     /locus_tag="VEA63_15325"
     CDS             complement(<1..>1503)
                     /locus_tag="VEA63_15325"
                     /inference="COORDINATES: protein motif:HMM:NF016497.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HYD85436.1"
                     /translation="HGADLPNPLIHQRADPWIHKHTDGFYYFTATVPEYDRLELRRSP
                     TIAGLASAETKTIWHKHPTGIMGAHIWAPELHHIDGKWFIYFAAGEAEKIWNIRIYVL
                     ENTSANPLEGEWIERGQLKTNWESFSLDATTFEHRGARYLVWAQRDPAVKNNTDLYIA
                     KMDTPTSITGRQVLLSRPEYDWEKIRYAVNEGPAVLHRHGRLWLTYSAAGTGAEYCLG
                     LLSADENADLLDPKSWTKSPTPVFTSSETNRIYGPGHNSFTVSEDGATDLLVYHARNY
                     REIVGNPLADPNRHTRVQPFAWRADGTPDFGEPAPEPLPKFGTVTRAELRARDACIFP
                     DPATQIYTMYFSSGQRGPNRRPAVVAYTSKDLEHWTGPTVVFETPADWWADRGIWAPE
                     MHAYRGKYYLFLTFDSSHKFPEQWRDWLPRVKRASQVLVADSPLGPFQPFANKPTLPE
                     DMMTLDGTLWEEDGVPYMVFCHEWVQIKDGTVEYVRLKNDLSATVGEPKR"
BASE COUNT          250 a          385 c          565 g          303 t
ORIGIN      
        1 ggcgcttcgg ctcgccgact gtcgcggaca aatcgttttt caaacggacg tattcgacgg
       61 tgccgtcctt gatctgcacc cattcgtggc agaagaccat gtaaggcacg ccgtcttcct
      121 cccagagcgt gccgtcgagc gtcatcatgt cctcgggcag cgtgggttta ttcgcgaacg
      181 gctgaaacgg cccgagcggc gaatcggcga cgaggacttg cgaggcgcgc ttcacgcgcg
      241 ggagccagtc gcgccattgc tcgggaaatt tgtgggacga gtcgaacgtg aggaagaggt
      301 agtattttcc gcgatacgca tgcatctcgg gcgcccagat gccgcggtcg gcccaccaat
      361 cggcgggcgt ttcgaagaca acggtcgggc cggtccagtg ttcgaggtct ttgctggtgt
      421 aagcgacaac agcggggcgg cggtttgggc cgcgctggcc ggaggaaaaa tacatcgtgt
      481 agatctgcgt cgcgggatcg ggaaaaatgc aggcgtcgcg ggcgcgcagc tcggcgcggg
      541 taacggtgcc gaacttcggc agcggttcgg gcgcgggttc gccgaagtca ggcgtgccgt
      601 cggcgcgcca ggcgaagggt tggacgcggg tgtgacgatt ggggtcggcg aggggattgc
      661 cgacgatctc gcggtagttt cgcgcgtgat acacgagcag gtcggtggcg ccgtcctcgc
      721 tgacggtaaa actgttgtgg cccgggccgt agatgcggtt ggtttcgctg ctcgtgaaaa
      781 cgggcgtcgg cgacttcgtc caggatttcg gatcgaggag gtcggcgttt tcgtcggcgg
      841 agaggaggcc gaggcaatat tcggcgccgg tgccggcagc ggaataagtg agccagagcc
      901 ggccgtggcg gtggaggacg gcggggcctt cgttgacggc gtagcggatc ttttcccagt
      961 cgtattcggg gcgcgaaagc agaacctgcc ggccggtgat ggacgtcggc gtatccattt
     1021 ttgcaatata gaggtcggtg ttgttcttca ccgcgggatc gcgttgggcc cagacgaggt
     1081 agcgggcgcc gcggtgttcg aacgtggtcg cgtcgaggga gaacgactcc cagttggttt
     1141 tcagctgacc acgctcaatc cactcgccct cgaggggatt ggccgaggtg ttttcgagga
     1201 cgtagatgcg gatgttccag atcttctcgg cttcgcccgc ggcgaagtag atgaaccatt
     1261 tgccgtcgat gtgatgaagc tcgggcgccc agatgtgcgc gcccatgatg ccggtcgggt
     1321 gtttgtgcca gatggttttg gtttcggcgc tggcgaggcc ggcgatcgtg ggcgagcggc
     1381 ggagttcgag ccggtcgtat tcggggacgg tggcggtgaa ataataaaag ccgtcggtgt
     1441 gtttatgaat ccacggatcg gcgcgctggt ggatgagcgg gttgggaaga tcggcgccgt
     1501 gag
//
LOCUS       DASPLT010001499         1015 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_222401, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001499 DASPLT010000000
VERSION     DASPLT010001499.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1015)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1015)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1015
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_222401"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            52..>1015
                     /locus_tag="VEA63_15330"
     CDS             52..>1015
                     /locus_tag="VEA63_15330"
                     /inference="COORDINATES: protein motif:HMM:NF014850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HYD85437.1"
                     /translation="MLSGDRSGANAIRLELFGPDGRLVARHETTLANALEGDRLQLEI
                     PLRDAPRWSPETPILHRAELTLLQDGLLIDRVDTRFGVRTIEIRGHEFIVNGVKYFIN
                     GYGDDAVFVDTIAPPSDKQFYLDRLKVAKSYGFNFVRHHSHFMPPEYYEACDEIGMFV
                     SPELPIAYPRYYDRAKGRALDLYRQEWAGAIVRYRNHPSIFNWCMGNELWEGIAIGPE
                     LYAMAKELDPTRAVLDSDGIFPPGFIDGTKDRPTKDYLTVMFDILTTPLENPRKFDTG
                     VPLKPIVTHEEGNFAFFPKLDEVPLYENTPFKPFWLTSTRDRVAR"
BASE COUNT          194 a          382 c          259 g          180 t
ORIGIN      
        1 caactcttcg ttcaaccaaa gcccgacgcg gatcgttgcg cggtctccgc catgctgtcc
       61 ggcgatcgct ccggcgccaa tgccatccgc ctcgagctct tcggccccga cggccgcctc
      121 gttgcccgcc acgaaaccac cctcgccaac gccctcgaag gcgaccgcct gcaactcgag
      181 attccgctgc gcgatgcacc gcgctggtcg cccgagacgc ccatcctcca ccgcgcggaa
      241 ctgacgctcc tgcaggacgg cctcctgatc gatcgcgtcg acacccgctt cggagtccgc
      301 acgatcgaga tccgcgggca cgagttcatc gtcaacggcg tcaagtattt cattaatgga
      361 tacggcgacg acgccgtgtt cgttgacacc atcgcaccgc cttcggacaa gcagttctat
      421 ctcgaccggc tgaaggtcgc gaagagctac ggcttcaatt tcgtccggca tcacagccat
      481 ttcatgccgc ccgaatacta cgaggcgtgc gacgagatcg gcatgttcgt ttcacccgag
      541 ttgcccatcg cgtatccacg ttactacgac cgcgccaagg gccgcgccct cgatctctat
      601 cgtcaggaat gggctggcgc gatcgtccgc taccgcaacc acccgtcgat cttcaactgg
      661 tgcatgggca acgaactctg ggagggcatc gccatcggcc ccgagctcta cgcgatggca
      721 aaggagcttg atcccacccg cgccgtcctc gacagcgacg gcatcttccc gcccggcttc
      781 atcgacggca ccaaggatcg cccgacgaag gactacctca cggtgatgtt cgacatcctc
      841 accacgccac tcgagaaccc gcgcaaattc gacaccggcg tccccctcaa accgatcgtc
      901 acccacgagg agggcaactt cgctttcttc ccgaagctcg acgaagtccc tctctacgag
      961 aacacaccgt tcaaaccttt ctggctcacg tccacccgcg accgcgtcgc gcgcc
//
LOCUS       DASPLT010001500         1487 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_263932, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001500 DASPLT010000000
VERSION     DASPLT010001500.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1487)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1487)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1487
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_263932"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..466)
                     /locus_tag="VEA63_15335"
     CDS             complement(<1..466)
                     /locus_tag="VEA63_15335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD85438.1"
                     /translation="MECSLVRIEGRVDDQLARSGHDQRLSDLDRFAALGLRAFRFPVI
                     WERHAGPTIDWSWSDARLEKLRSLGIRPIVGFLHHGCGPDVTCFLAPDFTTKLAHFAR
                     QVAERYPWIDAYTPINEPLTTARFSGMYGLWHPHRRDLASFARILLDECLATR"
     gene            complement(575..>1487)
                     /locus_tag="VEA63_15340"
     CDS             complement(575..>1487)
                     /locus_tag="VEA63_15340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 2"
                     /protein_id="HYD85439.1"
                     /translation="LPIDAPPIARIDPGSAFDIDVLSSHFSRRRCEGVTLHWLYSGVD
                     TLGTEHPNLARGRARIPFTHHRVELARRLSLVAPTQPMLCTLAVAAVLPDGHTVAGNY
                     VQQFVNNGPLPERDDQGNTLILRRRVTDWIDAEWSESHSTREEAGHAGACFGHGHGYF
                     EWEFTDEVIPLLAQAKRVRVFCEVSARRRDVPQTDSHRYPTYFELLINDLPVHRTLLP
                     DHPHDTRGALSYLRGGRGAYGYLMRAAFEGTLLEQVAATAARNGTLRFRCAVPAHTAP
                     HGGLTVYDFDSGRFPICPTIVIEWEQG"
BASE COUNT          275 a          431 c          540 g          241 t
ORIGIN      
        1 cacgcgtggc gaggcactcg tcgagcagaa tgcgcgcaaa ggacgcaagg tcgcggcggt
       61 gtgggtgcca aagtccatac attccgctga aacgtgcggt ggtgagcggc tcgttgatcg
      121 gcgtgtaggc gtcgatccaa ggataacgct cggcgacctg gcgggcgaaa tgcgcaagct
      181 tcgtcgtaaa gtccggcgcg agaaaacacg tcacgtccgg gccgcaaccg tggtggagga
      241 aaccgacgat cggacggatg ccgagcgacc gaagtttttc caggcgcgcg tcggaccagc
      301 tccaatcgat ggtcgggccg gcgtgccgct cccagatgac gggaaaacga aacgcgcgaa
      361 ggccgagcgc ggcgaagcgg tcgagatcgg agagccgttg atcgtggccg ctgcgggcga
      421 gctggtcgtc aacgcgcccc tcgatgcgaa cgagagagca ttccatccct ccccagattt
      481 ccagcggtgc aggtgctctc gatggtgttt cctccatgat cgcgcctgaa ccgtccgggc
      541 cagtcttgag gcgcagcccc actgaccgtt tccatcagcc ctgttcccat tcaatcacga
      601 tcgtcgggca tatggggaat cttccgctgt caaaatcgta gacggtcaac ccgccgtggg
      661 gcgcggtgtg tgcgggaacg gcgcagcgga aacgcagggt tccgttgcgc gcggcggtgg
      721 cggcgacttg ctcgaggagc gtgccttcga acgccgcgcg catgagatat ccgtaggcgc
      781 cgcggccgcc gcgaagataa ctgagcgcgc cgcgggtgtc gtgcggatga tcgggcaaca
      841 gcgtgcgatg aacggggaga tcgttgatca gcagttcgaa ataggtcgga taacgatggc
      901 tgtcggtttg cggaacgtcg cggcgtcgcg ccgacacttc gcagaacacg cgcacgcgtt
      961 tcgcctgcgc gagcagcggg atgacctcgt cggtgaactc ccactcgaag tagccatggc
     1021 cgtggccgaa acaagcgcca gcatggccgg cctcctcacg cgtggagtgg ctctcgctcc
     1081 attcggcgtc gatccagtcg gtgacgcggc ggcgaaggat gagcgtgttg ccctggtcgt
     1141 cgcgctcggg gagcgggccg ttgttgacga attgttgaac gtaatttccc gcgacggtgt
     1201 ggccgtcggg gagcacggcg gcgacggcga gcgtgcagag catcggctgc gtcggagcga
     1261 cgagcgagag gcggcgggcg agttcgacgc gatgatgcgt gaacggaatg cgcgcgcggc
     1321 cgcgagccag attgggatgc tcggtgccga gcgtatccac tcctgaatac agccagtgca
     1381 acgtgacgcc ctcgcagcgg cggcgggaga aatgcgatga cagcacgtcg atgtcgaacg
     1441 cggagccggg atcgatgcgc gcgatcggcg gggcgtcgat cggaagg
//
LOCUS       DASPLT010001501         1329 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_283651, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001501 DASPLT010000000
VERSION     DASPLT010001501.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1329)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1329)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1329
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_283651"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1300)
                     /locus_tag="VEA63_15345"
     CDS             complement(<1..1300)
                     /locus_tag="VEA63_15345"
                     /inference="COORDINATES: protein motif:HMM:NF024883.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HYD85440.1"
                     /translation="MSTSTAGPDQLLLDSDWRIEAVGAHAARRLRLTHLERAKIPGHR
                     LWEILPRLVGTRTETLLRTAMRSREPMNFEWLFLPEITLDVRVERASNSLILRVREIA
                     SSDRASLLAPPANASSRFTPAALANVASNPEPAYACSALGRFISANDLALELFDRAPA
                     AVVIGRKFTQLSLPRDSAAQLQHRIDLALLSGEPDVVEVLHPDGHGKLARYRHHLTPI
                     RDPEGGVRSLIGVARLAPDPSPAKPAEAPQPPARPEPLAPPTPVPAAATASDVNATSL
                     HRYHALCEVAVALPKDNLRKAPLFGALQRFARAIDAEIYLLHTASADGSQLRFEASGG
                     VSYEDLGTAANPAIGEGLCGVAARDDWPVVIEDVQHNDTPEAAEPRAWGARAVVCHPL
                     AIGSRLLGTLVFATTSRDRFSPDELAFMRDAAGLVAGSLET"
BASE COUNT          219 a          375 c          516 g          219 t
ORIGIN      
        1 gggtttccaa gctgccggcg acgagtcctg cggcgtcgcg catgaacgcc aactcgtcgg
       61 gcgagaagcg gtcgcgcgac gtggtggcga aaacgagcgt gccgaggagg cgggagccga
      121 tggcgagcgg atggcacacg acggcgcgcg cgccccacgc ccgcggttcc gccgcttcgg
      181 gagtgtcgtt gtgctgcacg tcttcgatga cgaccggcca gtcgtcgcgc gccgcgacgc
      241 cgcagaggcc ttcgccgatg gcgggattgg cggcggtgcc gaggtcttcg tagctgacac
      301 cgccgctggc ttcgaagcga agttgcgaac cgtcggccga agcggtgtgc aggagataaa
      361 tttctgcgtc gatggcgcgt gcgaaacgtt gcagcgcgcc gaagagcgga gctttgcgaa
      421 gattgtcttt cgggagagcg acagcgacct cgcagagcgc gtggtagcga tgcaggctcg
      481 tcgcgttcac atcgctcgcg gttgcggctg cgggaacggg tgtcggcggt gcaagcggct
      541 cggggcgcgc gggaggctgc ggggcttccg ccggtttcgc gggtgacggg tccggcgcga
      601 ggcgggcgac tcctatgagg gagcgcacgc cgccctcggg atcgcggatc ggtgtgaggt
      661 gatggcggta gcgggcgagt ttgccgtggc catccgggtg caacacctcg acgacgtccg
      721 gttcgccgga gagcagcgcg agatcgattc gatgttggag ttgcgcggcg ctgtcccgcg
      781 gcaaactcag ctgggtgaat tttcggccga tgacgacggc ggccggtgcg cggtcaaaaa
      841 gctcgagcgc cagatcgttg gccgaaatga agcgaccgag cgcgctgcac gcgtaggcgg
      901 gttcgggatt gctcgcgacg ttggccaatg cggcgggtgt gaagcgggag gacgcgttgg
      961 ccggcggagc cagaagcgat gcgcggtctg aggaggcgat ttcgcggacg cgaaggatca
     1021 gcgagttgga ggcgcgttcg acgcgaacat cgagcgtgat ctcaggcaga aacagccact
     1081 cgaagttcat tggctcgcgg ctgcgcatcg cggtgcgcag cagtgtttcc gttcgagtgc
     1141 cgacgagtcg cggaagaatc tcccacaatc gatggccggg aattttcgcg cgttcgaggt
     1201 gagttaaacg caatcgacgc gcggcgtgag cccccaccgc ttcgatgcgc caatcactgt
     1261 ccagaagcag ttgatccggt cctgcggtgc tggtggacac cacgcgacga aggggcgcgg
     1321 gtcgggttg
//
LOCUS       DASPLT010001502         2301 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_263944, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001502 DASPLT010000000
VERSION     DASPLT010001502.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 2301)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2301)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2301
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_263944"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..260)
                     /locus_tag="VEA63_15350"
     CDS             complement(<1..260)
                     /locus_tag="VEA63_15350"
                     /inference="COORDINATES: protein motif:HMM:NF016252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucan biosynthesis protein"
                     /protein_id="HYD85441.1"
                     /translation="MKTDYFWRGLKTKMAVGTSAATSLLAGLTLVSSAEAANGSFNFD
                     TVRYRAQVLAEKPYVEPASRVPEWLLKLTYDEHRHIRFDPGHT"
     gene            complement(257..1162)
                     /locus_tag="VEA63_15355"
     CDS             complement(257..1162)
                     /locus_tag="VEA63_15355"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HYD85442.1"
                     /translation="MKASGVKTVSVSDESVAALRRVLGGRKAVAPARAAAAVEARASV
                     EGEAPTRSAVTAVAREEAAPVKSEKKAAPVDESIPEPTPFTLPDGDKAARLAALRERV
                     LSDAVCCAHVRPGKKVVLGVGTLDAKIMFVGEAPGAEEEVQGEPFVGPAGQLLTKMIG
                     AMGLQRSDVYIGNIMNWRPEMALNASGVQYGNRPPTDQEMRYCLPYLRAQIEIVNPDL
                     IVALGSTATQGLLGAGSFKALGEVRGKWHAFAGKPLMVTYHPSYILRNQSNRSKRMIW
                     DDLLKVMEKAGLPISEKQRGYFLDK"
     gene            complement(1313..1909)
                     /locus_tag="VEA63_15360"
     CDS             complement(1313..1909)
                     /locus_tag="VEA63_15360"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HYD85443.1"
                     /translation="MFTGIVEETGRVVAFTAEAEAWRLQIAAGVVLGGVALGDSIAVN
                     GCCLTVAHFDAQHLWFDLLEETRQLTNFSALAAGSLVNLERSLRFDGKVGGHFVSGHI
                     DGLGTIDVFETRGKDYYLRVRAPAGSGRYLVHKGSIAIDGISLTVAEVEGDAFAVWLI
                     PTTLAVTNLRTRRVGEPVNLEFDLLGKYVEKLLPGRRE"
     gene            complement(2075..>2301)
                     /locus_tag="VEA63_15365"
     CDS             complement(2075..>2301)
                     /locus_tag="VEA63_15365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007363526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HYD85444.1"
                     /translation="DIGGVVAHFVKPNSPAAVAGLFTDDWIKEIDGVEVKTFASAVAQ
                     LAAIEADETRTEMVLLVSRGGETAVLRLKL"
BASE COUNT          450 a          865 c          546 g          440 t
ORIGIN      
        1 gtgtgccccg ggtcgaaacg aatgtgccga tgctcgtcgt aagtcagctt gagcagccac
       61 tccggcacgc ggctcgccgg ctccacgtaa ggtttctctg ccaacacctg cgcgcgataa
      121 cgcactgtat cgaaattaaa actaccattc gccgcctccg ccgacgacac cagcgtcagt
      181 cccgccagca gcgaggtagc ggcggacgtc ccgaccgcca ttttcgtttt caatccgcgc
      241 cagaaataat cggttttcat ttgtccaaaa aatacccgcg ttgcttctcc gaaatcggca
      301 agcccgcctt ctccatcacc ttcaacaggt cgtcccagat catccgcttc gagcggttcg
      361 actggttgcg cagaatgtaa ctcgggtgat aggtcaccat cagcggcttc ccggcaaacg
      421 catgccattt tccccgcact tcgcccaagg ccttgaacga tcccgcgccc agcagcccct
      481 gcgtcgccgt cgaaccgagc gccacgatca aatccggatt cacgatctca atctgcgccc
      541 gcaaatacgg caggcagtaa cgcatctcct gatcggtcgg cggacgattc ccatattgaa
      601 cgccactggc attcagcgcc atttccggcc gccagttcat gatgtttcca atgtaaacat
      661 cgctgcgttg tagtcccatc gccccgatca ttttcgtgag caattgtccc gccggtccca
      721 caaacggttc cccttggact tcttcttccg ctcccggcgc ctcgcccacg aacatgattt
      781 tcgcatccag cgtgccgacg ccgagcacaa ctttcttgcc cggccgcaca tgcgcgcaac
      841 acaccgcgtc gctcaagacc cgctcgcgca acgccgccag ccgcgccgcc ttgtcgccgt
      901 cgggtaacgt aaacggcgtc ggctccggaa tgctttcatc caccggcgcc gccttcttct
      961 ccgatttcac cggagccgct tcctcacgcg ccaccgcggt caccgccgaa cgcgtgggcg
     1021 cctcaccctc caccgaagct cttgcctcga ccgccgccgc agcccgcgca ggggccaccg
     1081 ccttgcgccc gccgagcacg cgacgcagcg ccgcaacact ctcgtccgac accgacacgg
     1141 ttttcacccc cgacgccttc aagcgtcgca attcctctgt caaagcagtg agcgcagcat
     1201 gcatagagat tcgcaaggta gcggcgatcg tcccgaccgc caccttcaag caaggcccgt
     1261 cgagacgccg tccgctacct ttttcaacag cttcccgccg ccagccccaa cttcactccc
     1321 gccggccggg cagcagcttt tccacgtatt tccctaacag atcgaactcg agattaaccg
     1381 gttcgccgac gcgccgcgtg cgcagattcg tcaccgcgag cgtcgtcgga atcagccaca
     1441 ccgcaaacgc atcgccctcg acttccgcca ccgtcagcga aatcccgtcg atcgcaatgc
     1501 tgcctttgtg cacgagatag cgtccgctcc ccgccggcgc gcgcacgcgc aaatagtaat
     1561 cctttccgcg cgtttcgaac acgtcgatcg tcccgagccc gtcaatatgt ccgctcacga
     1621 aatgcccgcc gacttttccg tcgaaccgca aactgcgctc cagattcacc agcgaacccg
     1681 ccgccagtgc cgaaaaattc gtcaactgcc gcgtttcctc cagcaggtcg aaccacagat
     1741 gctgcgcatc gaaatgcgcc accgtgagac agcagccgtt gaccgcgatg ctgtcgccca
     1801 gcgcgactcc gccgagcacg acgcccgccg cgatctgcag ccgccacgct tcggcctccg
     1861 ctgtgaaagc cacgacccgc cccgtttcct caacaatgcc agtgaacata gatgagttta
     1921 cttaaccacg aatggacacg aatcgacacg aatgaattcc accgtgccga taccgaggcc
     1981 gcgttttcca ttcgtgttca ttcgtgtcca ttcgtggtta aaaaattccg gcgttcgtcc
     2041 ttttcgtgtc catccgtggt tgaaaaaaac ctcctcacag cttcaaccgc aacaccgccg
     2101 tctccccgcc gcggctcacg agcaagacca tttccgtgcg cgtctcgtcc gcctcgatcg
     2161 ccgcgagctg agccaccgcc gacgcaaacg tcttcacctc cacgccgtcg atttccttga
     2221 tccaatcatc cgtgaacaaa cccgccaccg ccgccgggct gttcggcttc acaaagtgcg
     2281 cgaccacgcc gccgatgtcc t
//
LOCUS       DASPLT010001503         2126 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_182632, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001503 DASPLT010000000
VERSION     DASPLT010001503.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 2126)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2126)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2126
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_182632"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1335
                     /locus_tag="VEA63_15370"
     CDS             <1..1335
                     /locus_tag="VEA63_15370"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HYD85445.1"
                     /translation="IAVGAAAPHDNTGLIGRILTLRAEKAALLGKAHFADLVLERRMA
                     KTGERALAFLADLQKRAGEAFARECRELEEFKAQRTGGAVAALAPWELAYWSEKLRQE
                     RYAFDEEVLRPYFPLDRVIRGMFELAGRVFGLRITERPASEVETWHPEVKFYDVHDRD
                     GRHVGSFYADWHPRESKRGGAWMNYLITGGPRADGGRAPHLGLICGNLTPPAADRPAL
                     LTHREAETIFHEFGHLLHHLLGEVEIKSLNGVNVAWDFVELPSQIMENWCWERESLDL
                     FARHHETGAPIPEEIFAKMTAAKNFRSATGTMRQLSLARMDLLLHTRTAEFAAAADVE
                     PLARAAIAECLVPTQPPAPTVVKRFTHVFADPVGYAAGYYSYKWAEVLDADAFTRFKR
                     EGIFNAETGAAFVRNILSRGNSADPAELYRAFMGRDPDLNALLRRCGLAPAA"
     gene            1377..>2126
                     /locus_tag="VEA63_15375"
     CDS             1377..>2126
                     /locus_tag="VEA63_15375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD85446.1"
                     /translation="MRRSSTILASVGVAAVVAVATLPWWLGAAAGLIAPQFGLTFSDY
                     ERIGYSKFKLENVEVRRPGVYVQIDEAEAETPLVWLWRRATGNPSEVVGGAWRVEVDA
                     RAIPSKPEKPRGWVPLTQTLRRTAERLDRWLPRAKAGPGVVTWRNGELTLGPAGWRER
                     TLTASKVGFKALEAAGRFTLSEDGKMRLELDAPQNDGSAVLEAEGAKVAGEVTWWGVK
                     TVASAQFSDTGWRPLVANFRADAWEIPGSRAK"
BASE COUNT          358 a          546 c          829 g          393 t
ORIGIN      
        1 atcgcggtcg gcgcggcggc gccgcacgac aacacggggc tgatcgggcg gattttgacg
       61 ttgcgggcag aaaaggcggc gctgctcggc aaggcgcatt ttgcggattt ggtgctcgag
      121 cgacgcatgg cgaaaaccgg cgagcgtgcg ctggcgtttt tggcggattt gcagaagcgc
      181 gcgggcgaag cgttcgcgcg ggagtgtcgc gagttggagg agttcaaggc gcagcgcacg
      241 ggtggagcgg ttgcggcgct ggcgccgtgg gagctggcgt attggtcgga gaagttgagg
      301 caggaacgct acgctttcga cgaggaggtg ttgcgtccgt attttccgtt ggatcgcgtg
      361 attcgcggga tgttcgagct ggcgggacgt gtattcggtt tacggataac ggagcggccg
      421 gcgagcgagg tggagacctg gcatccggaa gtgaagttct acgacgtgca cgatcgcgac
      481 gggcggcacg tcgggtcgtt ttatgcggac tggcatccgc gcgagtcgaa gcgcggcggg
      541 gcgtggatga attatctgat cacaggtggg ccgcgggcgg atggcgggcg ggcgccgcat
      601 ctgggtctga tttgcgggaa tctcacgccg ccagcggcgg atcgaccggc cttgttgacg
      661 catcgtgagg cggagacgat ttttcacgag ttcgggcatt tgctgcatca cttgctcggg
      721 gaagtggaga tcaagtcgtt gaacggcgtg aacgtcgcgt gggacttcgt ggagctgccg
      781 tcgcaaatca tggagaactg gtgctgggag cgggagagct tggatttatt tgcgcggcat
      841 cacgagaccg gcgcgccgat tccggaggag attttcgcta agatgaccgc ggcgaaaaat
      901 ttccgttcgg cgaccgggac gatgcgacag ctctcgctgg cgcgaatgga tttgctgctg
      961 cacacgcgga cggcggagtt cgcggcggcg gcggatgtcg agccgttggc gcgcgcggcg
     1021 atcgcggagt gtctggtgcc aacgcagccg ccggcgccga cggtggtgaa acgctttacg
     1081 cacgtgtttg cggatccggt cggctacgcg gcgggctact attcctacaa gtgggcggag
     1141 gtgctcgatg cggatgcgtt cacgcgtttc aagcgcgagg gaatcttcaa cgcggaaacg
     1201 ggcgcggcgt tcgtgcggaa cattttgagc cgaggaaatt ctgccgatcc cgcggaactg
     1261 tatcgcgcat tcatgggtcg tgatccagac ctcaacgccc tcctgcggcg gtgcggtttg
     1321 gcgccggccg cttaaccggc acgcggcgcg cgagggacgc gagcgcttcc attttcatgc
     1381 gtcgttcctc caccattctc gctagtgtcg gcgtcgcggc ggtcgtcgcg gtcgcgacct
     1441 tgccgtggtg gctcggggcg gcggcggggc tgatcgcgcc gcagttcggg ctgacgtttt
     1501 ccgactacga gcggattggc tacagcaagt tcaaattgga aaacgtggag gttcgtcgac
     1561 cgggtgttta cgtgcagatc gacgaagcgg aagcggaaac gccgctcgtg tggctgtggc
     1621 gacgcgcgac gggaaatccg agcgaagttg tcggcggagc gtggcgagtg gaagtggatg
     1681 cgcgggcgat tcccagcaaa ccggagaagc cgcgcggttg ggtgccgctc acgcaaacgt
     1741 tgcgacgcac ggcggagcgg ctcgatcgct ggctgccgcg tgcaaaggcc gggccgggcg
     1801 tcgtcacgtg gcgcaacggc gagttgacgc ttggaccggc ggggtggcgc gaacggacgc
     1861 tgacggcctc gaaggtggga ttcaaagcgc tcgaagcagc ggggcggttt acgctgtccg
     1921 aagacggaaa aatgcggttg gagctcgatg ccccgcaaaa tgatggaagc gcggtgctcg
     1981 aggccgaagg cgcgaaggtg gcgggcgaag tgacgtggtg gggagtgaag acggtggcga
     2041 gtgctcagtt ttcggatacg ggttggaggc cgctcgtcgc gaactttcgc gcggacgctt
     2101 gggagatccc cggaagtcgc gcgaag
//
LOCUS       DASPLT010001504         3520 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_161639, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001504 DASPLT010000000
VERSION     DASPLT010001504.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 3520)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3520)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3520
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_161639"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..407
                     /locus_tag="VEA63_15380"
     CDS             <1..407
                     /locus_tag="VEA63_15380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD85447.1"
                     /translation="QATLPAELSPDSAGRYTATFTARDEGGYLATAEVTDRAGKFIGR
                     AEAGWVHDPAAEEFRSIVPNRALLEELARRTGGAVIDRSELAHLVDQLARTPAPIMET
                     WSRPIWHNAWIFAVVLGCFLAEWAWRRWRGLP"
     gene            404..1522
                     /locus_tag="VEA63_15385"
     CDS             404..1522
                     /locus_tag="VEA63_15385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD85448.1"
                     /translation="MNAAKHPHFLPPWRDRLRPIRFASSAANADVVSARGERPLPGLR
                     RRCIITLIGLILLFSFSAPPAAHAAEPVHEILLIVGAPGEGDYTEGFASAARAWEAAA
                     TRISAPITVIGLDETPSADAAVESDRDQLRAWCAAHETETRTPAWIVYLGHGTFDGRE
                     ARLNLRGPDVTAKEIATWLAPLDRPLVFIHGGSAGAPFISALSGPDRIIISATRSGHE
                     LNYARFGERFAEAVANPSADIDQDGQTSLLEAFVTAAQQVQAFYAETGRLATEHALID
                     DNGDHQGTPADWFRGVRLQRRPEGNAIPDGDAAKLIALIPSDAERALTSTQREARHRL
                     ERELDGLRARKSELPADEYYRQLESLFRRLGSIYRTDS"
     gene            complement(1666..3426)
                     /gene="polX"
                     /locus_tag="VEA63_15390"
     CDS             complement(1666..3426)
                     /gene="polX"
                     /locus_tag="VEA63_15390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009509294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase/3'-5' exonuclease PolX"
                     /protein_id="HYD85449.1"
                     /translation="MTKNEIADVLNEIALLLELKGENPFKVRAYQSGARVVESLEQAE
                     LERLIAANELQTVKGIGDALGQKISELQTTGKLEFYERLKASVAPGLVELLEIPSLGP
                     KKIKVLFETLGVADIASLTQACADGKVAGLPGFGAKTQEKILAGIKNREAYGRRHIWW
                     EAARVAGPILTGLRALPQVKRAESAGSLRRGMETVGDLDFIVAADEVAPVVEWFVAQP
                     GVQEVTAKGETKASVRFASGLQADLRIVPDEQFVFALHHFTGSKDHNVQMRQRALARG
                     LSLSEWGLLPAEGEGTVKRKAEDKTRRTVANTEAELFAALGLAFIPPELREGMGEIEA
                     AEAGKLPRLIEAADLRGVFHCHTTASDGRNTLSEMTAAAEALGWAYWGIGDHSKSSVQ
                     AKGLTEERLAKQIEEIAALNASGRFKTHVFTGSECDILPDGRMDFDDGLLAKLDYVVA
                     SVHSTFTQDEATMTARIIRAIEHPRTTMLGHLTGRLLLRREGYRVDTTKIIDAAIANR
                     VIIEINASPWRAEMDWRFWRKAAERGLLCSINPDAHETADLGYVSAGVNVARKGWLTK
                     AHVLNTRTLEEVKAYLAARK"
BASE COUNT          608 a         1443 c          838 g          631 t
ORIGIN      
        1 aacaagccac cctccccgcc gaactctcgc cggattccgc cggtcgctac acggcgacgt
       61 tcaccgcgcg cgacgagggc ggctatctcg ccaccgccga ggtcaccgat cgcgccggaa
      121 agttcatcgg tcgcgccgaa gcgggctggg ttcacgatcc ggccgccgag gaatttcgct
      181 cgatcgttcc gaaccgcgcc ttgctcgaag aactcgcccg ccgcaccggc ggcgccgtca
      241 tcgatcgcag cgaactcgcc cacctcgtcg atcagctcgc gcgcacgccc gctccgatca
      301 tggaaacctg gtcccgcccg atctggcaca acgcctggat cttcgccgtc gtcctcggct
      361 gcttcctcgc cgagtgggcc tggcgccgct ggaggggcct gccatgaacg ctgccaaaca
      421 tccgcacttc cttccgccat ggagggaccg gctccgtccg atccggtttg catcctccgc
      481 ggcaaacgcg gacgtcgtct ccgcacgcgg agaacgacct ctccccggtc tgcgtcgtcg
      541 ctgcatcatc accctcatag gcttaattct cctcttctcg ttctccgcgc ctcccgccgc
      601 ccacgccgcc gaacccgtcc acgaaatcct cctcatcgtc ggcgctcccg gcgaaggcga
      661 ttacaccgaa ggcttcgcct ccgctgcgcg cgcctgggaa gccgccgcca cgcgtatcag
      721 cgcacccatt acagtcatcg gcctcgatga aacaccctcc gccgatgctg ccgtcgaatc
      781 cgaccgcgac caactccgcg cctggtgcgc cgcgcacgaa accgaaaccc gcacccccgc
      841 ctggatcgtt tacctcggcc acggaacgtt cgacggccgc gaagcgcgtc tgaacctccg
      901 cggccccgac gtcaccgcca aggaaatcgc cacctggctc gcgccgctcg atcgccccct
      961 cgtcttcatc catggcggct ccgccggcgc accgttcatc tccgcgctgt ccggccccga
     1021 tcgtatcatc atttctgcga cacgcagcgg tcatgagctc aactacgccc gcttcggtga
     1081 acgcttcgcc gaagccgtcg ccaacccctc cgccgacatc gaccaggacg gacaaacctc
     1141 cctcctcgag gccttcgtca ccgccgcgca gcaagtccag gccttctacg ccgaaactgg
     1201 ccgcctcgcc actgagcatg ccttgatcga cgacaacggc gaccaccagg gcacgccggc
     1261 tgattggttt cgtggcgtgc gattgcaacg ccgccccgaa ggcaacgcca tccccgacgg
     1321 cgacgccgcc aagctcatcg cgctcattcc cagcgacgcc gaacgcgcgc tcacctccac
     1381 ccaacgcgaa gcacgccacc gcctcgaacg cgaactcgac gggctccgcg cccgcaaatc
     1441 cgaactcccc gccgacgagt attaccgcca gctcgaatcc ctcttccgcc gcctaggctc
     1501 tatctaccgc accgattcat aatagatagg agtctgcttg caggcgaccg gactctcccc
     1561 cgtggctcgc tgcgtgagca gcgggaatgg acgccctccg acgcccacaa atcgcccgca
     1621 agcaggctcc taccccaacc ctgcgaatcg cgctacgccc tcccgtcact tccgtgccgc
     1681 caaatacgcc ttcacctcct ccagcgtccg cgtgttcaac acgtgcgcct tcgtcagcca
     1741 tcctttgcgc gccacattca cgccggcgct cacatagccc aaatcagcgg tctcgtgcgc
     1801 gtccggattg atcgagcaca gcagcccgcg ctccgccgcc ttccgccaga aacgccagtc
     1861 catctccgcg cgccacgggc tcgcgttgat ctcgatgatt acccgattcg cgatcgccgc
     1921 atcgatgatc ttcgtcgtgt ccacccgata gccttcgcgc cgcaacaaca accgccccgt
     1981 gagatgcccg agcatcgtcg tccgcggatg ctcgatcgcg cgaatgatcc gcgccgtcat
     2041 cgtcgcctcg tcctgcgtga acgtgctgtg caccgacgcc accacgtaat ccagtttcgc
     2101 cagcaacccg tcgtcaaaat ccatccgccc gtccggcaat atatcgcatt ccgatccagt
     2161 gaacacatgc gtcttgaatc gcccgctcgc gttcagcgcc gcgatctcct cgatctgttt
     2221 cgccagccgc tcctccgtga ggcccttcgc ttgcacgctt gatttcgagt ggtctccaat
     2281 cccccaatac gcccacccga gcgcctccgc ggccgccgtc atttcgctca acgtatttcg
     2341 tccatccgac gccgtcgtgt gacaatgaaa caccccgcgc aaatccgccg cctcgatcaa
     2401 ccgcggcagc ttccccgcct ccgccgcctc gatctcgccc atcccctcgc gcaattccgg
     2461 tggaataaac gccagcccca gcgccgcgaa cagctccgcc tccgtgttcg ccaccgtccg
     2521 ccgcgtcttg tcctccgcct tccgtttcac cgtgccttcg ccttccgccg gcaacaatcc
     2581 ccactcgctc aaactcaacc cacgcgcgag cgcccgttgc cgcatctgca cgttgtgatc
     2641 tttcgaaccg gtgaagtggt gcagcgcaaa aacaaactgc tcgtccggca cgatccgcaa
     2701 atccgcctgc agcccgctcg cgaaccgcac gctcgctttc gtttcacctt tcgccgtcac
     2761 ctcctgcacc cccggctgcg cgacaaacca ctcgaccacc ggcgccactt cgtccgccgc
     2821 cacgatgaaa tccaaatcgc ccaccgtctc catcccgcgc cgcagactcc ccgccgactc
     2881 cgcccgcttc acctgcggca acgccctcaa tcccgtcaaa atcggccccg ccacccgcgc
     2941 cgcctcccac caaatgtgac gccgcccgta agcctcgcga ttcttgatcc ccgccaaaat
     3001 tttctcctgc gtcttcgcgc caaagcccgg cagccccgcc acctttccgt ccgcgcacgc
     3061 ctgcgtcagc gacgcgatgt ccgccacgcc aagcgtttcg aacagcactt tgattttctt
     3121 cggcccgaga ctcggaattt ccaacaactc cacgagtccc ggcgcgaccg acgccttcaa
     3181 ccgctcataa aactccagct tcccggttgt ctgcagctcg gagattttct gtcccaatgc
     3241 gtcgccgatt cctttcaccg tctgcagttc attcgccgcg atcaaccgtt cgagttccgc
     3301 ctgttcgagc gactccacca cgcgcgcgcc ggattgatac gcacgcacct tgaacgggtt
     3361 ttcgcccttc aactcgagca acagcgcaat ctcgttcaac acatccgcaa tctcgttttt
     3421 ggtcacgccc gcaaacaacc acgattcccc cgaaacacac gaaatttttc atcccccgta
     3481 gcggcaggcc tccgtgcctg cctgccttaa actcgctcgc
//
LOCUS       DASPLT010001505         2694 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_283714, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001505 DASPLT010000000
VERSION     DASPLT010001505.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 2694)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2694)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2694
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_283714"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(36..446)
                     /locus_tag="VEA63_15395"
     CDS             complement(36..446)
                     /locus_tag="VEA63_15395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YXWGXW repeat-containing protein"
                     /protein_id="HYD85450.1"
                     /translation="MKTSKFIPLLALGGAAAFFGSGCATEPESNVVSAPPPGAPAMAS
                     SQPVVVATPSATTQGQSTIVVTQSPPAAQSEVVPQRPSSNHVWVPGYWTWRNNRYEWI
                     AGRWTMPPHSGATWIPPRWDQEGGAYRYYEGYWD"
     gene            complement(784..2298)
                     /locus_tag="VEA63_15400"
     CDS             complement(784..2298)
                     /locus_tag="VEA63_15400"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HYD85451.1"
                     /translation="MDKNKRLFIASVVAIATTSFGFIVRAFLITEWGVTFNLSDTQIG
                     ALQGAGLFPFALSIILFSLFIDRIGYGRAMAISWIGHVVSAVVTITATSYTQLYVGTL
                     IFALANGGVEAVTNPVVATLYPREKTKYLNILHAGWPGGLVLGGLLFIALGSISWQFK
                     IGLFLLPAMVYGVMLLGTKFPVQERVAAGVSYADMLKEFGWAGCLIVSYFVAGAVEAV
                     STGIFHSVLPTWAFWTITLVPAAIFAFNVKSFGRPMFVFMLLIMVLLATTELGTDSWV
                     SALMTPVLKNLGANAGNWVLVYTSAIMFVLRFFAGPIVHKISPLGLLAVCSLLAAGGL
                     LALANAGAAAGMVFLAATLYGVGKSFFWPTTLGVVSEQFPRGGALTLNAMGGMGMIAV
                     GVLGAPFLGALQDKSYDTAVQEAQPAIHATVAADEQRAFLMNFRPLDKQKIASLPADQ
                     QAVLEELRAETNQSTLAKFAILPMIMAACYGVLLLYFKSRGGYKPVDLGTTPAH"
BASE COUNT          580 a          946 c          745 g          423 t
ORIGIN      
        1 tggtcacgcg cagctcacgc gaaacgttcc gcgtctcaat cccagtaacc ttcgtagtaa
       61 cgatacgcgc caccttcctg atcccaacgc ggcggaatcc aagtcgcgcc ggaatgcggc
      121 ggcatggtcc agcggccggc gatccattcg taacgattat tacgccaggt ccagtagcca
      181 ggcacccaaa cgtggttcga ggacggacgc tgcggaacga cttccgattg cgccgccggc
      241 ggcgattgcg tgacgacgat cgtgctctgc ccttgggtcg tcgcactcgg cgtggcgaca
      301 acgaccggct ggctcgaggc catggccggc gcgcccggcg gcggtgcgga gacgacgttg
      361 gattccggtt cagtcgcgca gccgctgcca aaaaaggccg ccgcaccgcc caacgcgaga
      421 agggggataa actttgaggt tttcataacc cgcgtcactg accgttgaaa cgagatgcgg
      481 ttcatcagca aaatcccgaa gcctgccctc atcgctcgcg aacccgtcgc ccgttccttt
      541 tccgtcgaat gcaatagcac gtggagcccg acgtcctcat cgggctgttt gcgaacgaaa
      601 cgtcatcaag cccgacgagg acgtcggact ccacctttcg gtgcgcactt tcctcgccgg
      661 ccgagcggct tccactgcat gagcccggca cagccgcccc cttctcgccg ccaacaaaaa
      721 ggccgcgctc cgtgcgcggc cttggtcagc ccattaaggc tcttggctaa agcaacctcc
      781 ggcttagtgg gcgggcgtcg tccccaaatc cacgggtttg tagccgccgc gcgatttgaa
      841 atacagcagc agcacgccgt aacacgccgc catgatcatc ggcagaatgg cgaacttcgc
      901 cagcgtcgac tggttggtct ccgcgcgcaa ttcctcgagc actgcctgtt gatccgccgg
      961 cagcgacgcg atcttctgtt tgtccagcgg acggaaattc atgaggaacg ccctctgctc
     1021 gtccgcggcg accgtcgcgt ggatcgccgg ctgcgcttcc tgcaccgccg tgtcgtagga
     1081 cttgtcttgc aacgcaccga ggaacggcgc gccgagcacg cccaccgcga tcatgcccat
     1141 gccgcccatc gcattaagcg tcagcgcacc gccacgggga aactgctctg aaaccacccc
     1201 gagcgtcgtc ggccagaaaa acgatttgcc cacgccgtag agtgtcgccg cgaggaagac
     1261 catgcccgca gccgcgccag cattcgccag cgccagcagt ccgcccgccg ccagcaacga
     1321 gcacaccgcc agcaaaccga ggggcgaaat cttgtgcacg atgggcccgg cgaaaaaccg
     1381 cagcacgaac atgatcgccg acgtgtaaac gagcacccag ttgcccgcgt tcgccccgag
     1441 gtttttcagc accggcgtca tcagcgcgga aacccagctg tccgtgccca actccgtcgt
     1501 ggcgagcagc accatgatca acagcatgaa cacgaacatc ggccggccga agctcttcac
     1561 gttgaacgcg aagatcgccg ccggcaccag cgtgatcgtc cagaacgccc acgtcggcag
     1621 caccgagtgg aagatacccg tgctgaccgc ttcgaccgcg cccgccacaa aatacgaaac
     1681 gatcaagcag cccgcccagc cgaactcctt caacatatcc gcgtaagaaa cacctgcggc
     1741 gacgcgttcc tgcaccggga atttggtccc gagcaacatc acgccgtaga ccatcgcggg
     1801 cagcaggaag agaccgatct tgaactgcca gctgatcgag cccagcgcga tgaacagcaa
     1861 accgcccagc accaagccgc ccggccagcc tgcatggaga atgttgagat acttcgtctt
     1921 ttcccgcgga tacagcgtgg cgaccaccgg attcgtcacg gcttcgacgc caccgttcgc
     1981 gagcgcaaaa atcaacgtcc cgacgtagag ctgcgtgtaa ctcgtcgccg tgatcgtgac
     2041 cactgccgac accacgtgac cgatccacga aatcgccatc gcccggccat aaccgatccg
     2101 gtcgatgaac agcgaaaaca ggatgatgct cagcgcaaag gggaacagac ccgccccctg
     2161 cagcgctccg atttgcgtat cggagagatt aaatgtcacg ccccactccg taatgaggaa
     2221 ggcgcgcacg atgaagccga acgaggtggt ggcgatggcg acaacactcg cgataaacag
     2281 acgtttattt ttgtccatgg ggggttggaa gagcagctag acaggccgtc cgggaggtcc
     2341 ggcacggtga aagcgcgacg actcaaagtg ggaaacgttg ccgtgtccaa gcgcaaaaat
     2401 ttccgagcgc caaaatgccg ccccccagta gcggcaggcc tccgcgcctg ccagcccaac
     2461 cctttcgaaa tccaaacccg tcacggctca ccacggctca acccagcgcc cacccacaca
     2521 cgccacccac actcacgccc gaatcctccc gccacctcgc gcgccgccaa cctcagacct
     2581 tcacttcaac gcgcttccca tccgccgcgg ccgaagcgtc gatcgcttgc atgatcgcga
     2641 catgatcgcg ccccaattca cccggcaccg gcgtagcgcg ctcctcgatc acgc
//
LOCUS       DASPLT010001506         1341 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_182710, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001506 DASPLT010000000
VERSION     DASPLT010001506.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1341)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1341)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1341
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_182710"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..346
                     /locus_tag="VEA63_15405"
     CDS             <1..346
                     /locus_tag="VEA63_15405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HYD85452.1"
                     /translation="ISDDVLDRLDALLGHPTTDPHGDPIPSAQGKLHSQIYATLATCA
                     LAKPLRIVRVTEQSAEFLQFAEQNGLLPGATVRVVDRNLAAGLVTLKRSSGQTAALSL
                     AAAGKILVEPVK"
     gene            525..>1341
                     /locus_tag="VEA63_15410"
     CDS             525..>1341
                     /locus_tag="VEA63_15410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HYD85453.1"
                     /translation="MSQSFARTIFAVTAAFFAAFFLWPILQILKGGFIDADGDLTFAY
                     LTALLADPTYLTALRNSFLLACATTTLALLLALPLAFVSDRFLYPGKSLLGSLILIPM
                     ILPPFVGAIGIKQIFGQYGAFNSLLIALNLRPDGWAFDWFAVSQFWGVAVVQALSLYP
                     IIYLNAVAALANIDPAMEEAAQNLGCTGFRRFFKITLPLINPGLFAGGTIVFIWSFTE
                     LGTPLIFDYARVTSVQIYYGLRDIGGNPFPYTLVAVMLATSIALYAIGKGLFGR"
BASE COUNT          197 a          554 c          305 g          285 t
ORIGIN      
        1 gatctccgac gacgtccttg accgtctcga cgcgctgctc ggccatccca ccaccgatcc
       61 gcacggcgac cccattccca gcgcgcaagg caaactccac tcgcaaatct atgcgacgct
      121 cgcgacgtgc gctctcgcga aaccccttcg catcgtccgc gtcaccgagc aatccgccga
      181 attcctgcaa ttcgccgagc aaaacgggct cctccccgga gcaaccgtgc gcgtcgtcga
      241 tcgcaatctc gccgccggcc tcgtcaccct aaagcgctcc tccggccaaa ccgccgccct
      301 cagcctcgcc gccgccggca agatcctcgt cgaacccgtc aagtagggcg cggcttcagc
      361 ccgccttccc cggcattcgc cgacctccgc ggttacaacc atctcgctct ttctttgcgc
      421 cttctgcgcc tctttgcggc cattctctca gctcacgatt tttcccccgc cccgccgcgt
      481 tcccccttgc ccgcccccgc ttcgcggcac cactctcgcg cccgatgtcg cagtctttcg
      541 cgcgcacgat cttcgctgtc accgccgcct ttttcgcggc gttttttctg tggccgatcc
      601 tccagattct caaaggcggc ttcatcgacg ccgacggcga cctcactttc gcctacctca
      661 ccgcgctgct cgccgatccg acttatctca ccgcgctgcg caactccttc ctgctcgcgt
      721 gcgccacgac cacgctcgcg cttcttctcg cgcttccgct cgccttcgtc agcgaccgtt
      781 ttctctaccc cggaaaaagt ctcctcggtt cgctcatcct gatcccgatg atcctgccgc
      841 cgttcgttgg cgccatcggc atcaaacaaa ttttcggcca atacggcgcg tttaactcgc
      901 ttctcatcgc cctcaacctc cgccccgacg gctgggcctt cgattggttc gccgtcagcc
      961 agttctgggg cgtcgccgtc gtgcaggcgc tttcgctgta tcccatcatc tatctcaacg
     1021 ccgtcgccgc gctcgccaac atcgatcccg cgatggaaga agccgcacaa aacctcggct
     1081 gcaccggctt ccgtcgcttc ttcaagatca cgcttccgct catcaacccc ggcctcttcg
     1141 ccggcggcac catcgtcttc atctggtcgt tcaccgagct cggcacgccg ctgatcttcg
     1201 actacgcccg cgtcaccagc gtccaaatct actacggtct ccgcgacatc ggcggaaacc
     1261 ccttccccta cacgctcgtc gccgtgatgc tcgccacctc catcgcactc tacgcgatcg
     1321 gcaaggggct cttcggccgc a
//
LOCUS       DASPLT010001507         1946 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_161693, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001507 DASPLT010000000
VERSION     DASPLT010001507.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1946)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1946)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1946
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_161693"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..683)
                     /locus_tag="VEA63_15415"
     CDS             complement(<1..683)
                     /locus_tag="VEA63_15415"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HYD85454.1"
                     /translation="MIGEIFPARTIAIIGGGFSGSALAINLLRRATTSLRVVLIERKP
                     PFGGGVAYGTKSPDHLLNVPADKMGLFPDEPRDFVRWLREHREVEGVPETFGETDFLP
                     RRLYGAYVASLLNEAEAEAASDVDLTRVEGEVVDLEERAGGGAILRLQDGNEVFADQV
                     VLALGNLPGAYPIPRPLPIYHSSRYVHVPWRADALGGIGPEDDVLLVGQGLTASDLIV
                     ELDRRGHRGT"
     gene            complement(795..1781)
                     /gene="cysK"
                     /locus_tag="VEA63_15420"
     CDS             complement(795..1781)
                     /gene="cysK"
                     /locus_tag="VEA63_15420"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HYD85455.1"
                     /translation="MAKIHNDITETIGNTPLVRLNRTAAAHGAQAEILLKLEFFNPLS
                     SVKDRIGFAMIEDGLKSGKITKDSVLIESTSGNTGIALAFVAAAKGLKLILTMPETMS
                     IERRKLLKILGARLVLTEGAKGMKGAIAKAEELAAKIPNSIIMQQFANPANPAIHRKT
                     TAEEIWNDTDGKVDIIVSGIGTGGTITGVGEVLKPRKPSLKIVAVEPDASPILSGGTP
                     GPHKLQGLGAGFIPAVLNTKVYDEVIRVKEVDAGPVSKQVNQLDGIPVGISSGAAVWA
                     AIQLAKRPENKGKQIVAIIPSSTERYLSTWLFADVNTESDSIDDLLSASAAT"
BASE COUNT          376 a          626 c          570 g          374 t
ORIGIN      
        1 gtcccgcgat gcccgcgtcg atcaagttcg acgatcaaat ccgaagccgt gagcccctgc
       61 cccaccagca gcacgtcgtc ttccggcccg attccgccca gcgcgtccgc acgccacggc
      121 acatgcacat aacgcgagct gtgataaatc ggcaacggac gcggaatcgg atacgcaccc
      181 ggaagatttc ccagtgcgag cacgacttgg tctgcaaaca cttcgtttcc atcctggagc
      241 cgcagaatcg cgccgccgcc cgcgcgctcc tccaaatcca cgacctcgcc ttcgacgcgc
      301 gtcaggtcga cgtcactcgc cgcctccgct tcggcttcgt tcaacaagga agcgacatac
      361 gcgccataca accgtcgcgg cagaaaatcg gtctcgccaa acgtctccgg cacaccctcg
      421 acctcgcgat gctcgcgcaa ccaacggacg aaatcgcgcg gctcatccgg gaaaagtccc
      481 atcttgtcgg ccggcacgtt gagcaggtgg tccggcgatt tcgtgccata tgcgacgccg
      541 ccgccaaagg gcggcttgcg ctcgatcagc acgacccgca acgaagtcgt ggctcgacgc
      601 agcagattga tcgcgagcgc cgagccgctg aatccaccgc caatgatggc gatcgttcga
      661 gcaggaaaaa tttctccgat catgggaaaa gaaaaaacac ggttgccgac gtccgcggtt
      721 cgggaagttc cacgggcgac gttggggaaa gcacgacgcg tgctcggtaa acggcaaatc
      781 ccggcgacgc cggatcaggt cgccgcggaa gccgacagca ggtcgtcgat cgaatcgctc
      841 tcggtgttca catcggcgaa caaccacgtc gacagataac gctccgtgct cgacggaatg
      901 atcgcgacga tctgcttgcc cttgttctcg ggccgcttcg ccagttggat cgccgcccac
      961 accgccgcac ccgaggagat gccgacggga atgccgtcga gttggttcac ctgcttcgag
     1021 accggaccgg cgtcgacttc cttcacgcga atcacttcgt cgtaaacctt cgtgttgagc
     1081 accgccggaa tgaaacccgc gccgagcccc tgcaacttgt gcggaccagg cgtgccaccg
     1141 gaaagaatcg gcgaagcatc cggctccacc gccacaatct tcagcgacgg tttgcgcggt
     1201 ttcagcactt cgccgacgcc cgtgatcgtg ccgcccgtgc cgatgcccga cacgatgatg
     1261 tccaccttgc cgtccgtgtc gttccagatt tcttccgccg tcgtcttgcg atgaatcgcg
     1321 gggttcgccg ggttcgcgaa ctgctgcatg ataatgctat tgggaatctt cgcggcgagc
     1381 tcctccgctt tcgcgatcgc gcccttcatg cccttcgcgc cttcggtgag gaccaggcgc
     1441 gcgccgagaa ttttcaacag cttgcggcgc tcgatgctca ttgtctccgg catcgtgaga
     1501 ataagcttca gccctttcgc cgcggcgacg aacgccagcg cgatgcccgt gttgccggag
     1561 gtggactcga tcagaacgga gtctttcgtg atttttccgg acttcagccc gtcttcgatc
     1621 atagcgaaac cgatgcggtc cttcacgctc gagagcggat tgaagaactc cagcttaaga
     1681 agaatctccg cctgcgctcc gtgggcggcg gcggtgcgat tgaggcgcac gaggggggtg
     1741 ttgccgatgg tctcggtgat gtcgttatgg atttttgcca tgatcgtgat gtgggttgtt
     1801 gggagaaaaa ttgcaaccga ccggcgccgg aaggccgttc tagattgtgg cataaaccgt
     1861 gtgtggcaat gcgcgagata agacacgcgc ggtggatttt ccactcggcg tgacgccagc
     1921 agccgctttg ctcggcgtga tgacct
//
LOCUS       DASPLT010001508         1166 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_202959, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001508 DASPLT010000000
VERSION     DASPLT010001508.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1166)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1166)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1166
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_202959"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..879)
                     /locus_tag="VEA63_15425"
     CDS             complement(<1..879)
                     /locus_tag="VEA63_15425"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HYD85456.1"
                     /translation="MVTGSNLPSAGETPVAPLTIFSTTDIARSGVTNDLLQVIRKVAP
                     QFSGSGNLGGANANISSGSTNGGSQLALRNLATLVLVNGRRLAAAPVAATGGEVFVDV
                     NAIPVSAIARIEILTDGASAIYGSDAVSGVVNIILKTDFEGAEIGGRFGRTDNLGHYS
                     ERSAWGVVGGQMGEKGPRLTAAYEWSRTDPLYNFQRPFATPSYGTTNFAGVIQLGELD
                     AAGNFIGDPNGYYYLDLTLSAPGAGTTLAERGYDGPLEVGTVLRTFDLSRFVTMLIGS
                     EKNIFTLSGDQRFSERL"
     gene            803..973
                     /locus_tag="VEA63_15430"
     CDS             803..973
                     /locus_tag="VEA63_15430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD85457.1"
                     /translation="MSVVEKIVNGATGVSPALGRFEPVTTNDSNFTGSSSARAIPHAN
                     IVAAANKKKSSG"
BASE COUNT          227 a          435 c          299 g          205 t
ORIGIN      
        1 caaccgttct gaaaacctct ggtcgcccga gagcgtgaaa atgttctttt cgctcccgat
       61 cagcatcgtc acgaaccgcg acagatcgaa cgtccgcaaa accgtcccca cctcgagcgg
      121 cccgtcgtag ccgcgctccg ccagcgtcgt ccctgcgccc ggcgcgctca acgtcagatc
      181 gagatagtag tagccgttcg gatcgccgat gaagtttccc gccgcatcga gttcgcccag
      241 ctgaatcacg cccgcgaaat tggtcgtgcc atagctcggc gtcgcgaacg ggcgctgaaa
      301 attgtagagc ggatccgtcc ggctccattc gtaagcggcc gtcaaccgcg gccccttctc
      361 acccatttgc ccgccgacga cgccccacgc cgaccgttca ctgtagtgtc ccagattatc
      421 cgtgcgtcca aatcgcccgc caatctctgc gccttcgaaa tccgttttca aaatgatgtt
      481 caccaccccg ctcaccgcat cgctgccgta aatcgccgac gcaccgtccg tcagaatttc
      541 gatgcgcgcg atcgcgctca ccggaatcgc attcacgtcc acaaacacct ccccgcccgt
      601 cgctgcgacc ggcgccgccg cgagccgccg gccgttgacg agcacgaggg tcgccagatt
      661 tcgcaacgcg agttgcgacc cgccgttcgt gctcccactg ctgatgttcg cgttcgcgcc
      721 acccaaattt ccgctgcccg agaactgcgg tgcgactttc cgaataacct ggagcagatc
      781 gttcgttact ccgcttcgcg caatgtctgt ggtcgaaaag atcgtcaacg gcgcgaccgg
      841 cgtctccccg gcgctcggca gatttgagcc cgtgaccacc aacgactcca atttcaccgg
      901 ctcgtcctcg gcccgcgcga tcccgcacgc caacatcgtc gccgcagcga acaaaaagaa
      961 gagcagcggt tgactcaacc gtcctgagca gaagacgctc ccaccgcaac tccgcagcat
     1021 gttggccaca gtcgtcgcac cgcgaccgcc aacaatcgca ttgcgaccga caccggttgg
     1081 accgcgccga atccacccgg gaccgcgccg gaaccgcatc ccagccacgc ggctagctgc
     1141 ccgcgagctg cgacgaatat ctcatt
//
LOCUS       DASPLT010001509         2062 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_202966, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001509 DASPLT010000000
VERSION     DASPLT010001509.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 2062)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2062)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2062
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_202966"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            136..>2062
                     /locus_tag="VEA63_15435"
     CDS             136..>2062
                     /locus_tag="VEA63_15435"
                     /inference="COORDINATES: protein motif:HMM:NF013508.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HYD85458.1"
                     /translation="MAANQAANTVSVLFGQGTGVLSGRIDLAVKNAPYSPVIGDFDGD
                     GHLDIAVANASSNSVSVLRGNGAGAFATQLQSSAGAGVRALATADFNGDGYLDLATAN
                     YNGATASILIGKGNGAFNAPVLLNVGPNPTAVAAGDLNRDDRPDLAVTCAGSNDVRVF
                     LANGGGTFASASAYPTGSAPSAVAIADLDNDGWPDLAVTNENSNSVSILNGTGSGAFA
                     ASPNAIAVSPRPLGITTGHLDSDHVLDLVVASATNNMLTVLGGNGDGTFRSPAVIPAG
                     VFSRAVALADFNADSRLDVAVGNMNGNDVWIVLNQTGTIADLAVSVSNYASSLRSGAP
                     VSYEAVVSNLSSADVSELNLQLTVPSALVSPAFTPSAGAYDPATGAWTGLSLGGGQQA
                     VLTIAGTVSEDAAGTIKLEAIVGPGAGYTDPVAGNNTAEDLDVLIRGDADLQVAISNG
                     RTSVAAGDDVSYTITVSNVGPIAVQGAHVTTLLPGALRNVAWSCTASAGAVCPPAGSG
                     AIDELLSLPVGGSATFVLAAFIDERVASGTLAVSASVAAPSDVIDPVAENDAATDEDA
                     LSGDAPPVAASQSLTLSEDGSVQVEVAATDADGDALTFSVVTPPAHGVLSGTAPYFTY
                     TPAANYSGADTFVFQADD"
BASE COUNT          358 a          659 c          674 g          371 t
ORIGIN      
        1 cgtgacggtc acagcgtctc cgtgctgcgc ggcagaggcg cagtcggttt ccaagccccc
       61 agggcgtttg cctcgggtgc gaacccgcgt ggcgtcgcaa ccggcgacct gaacggggat
      121 gggcacgccg acgtagtggc ggccaatcag gccgcgaaca cggtgtcggt gcttttcggg
      181 cagggaaccg gggtactctc gggacgaatc gatctggcgg taaagaacgc tccatactcg
      241 cccgtgatcg gcgatttcga tggagacggg cacctggaca ttgctgtcgc caacgcgagt
      301 tccaacagtg tttcggtgct acgtggaaac ggtgcaggcg ccttcgcaac ccaactgcaa
      361 tcgtctgccg gggcgggcgt gcgcgcactt gccacggcgg atttcaacgg cgacgggtac
      421 ctggacctcg caaccgcgaa ctacaacgga gcaaccgcat ccatactcat cggtaaaggg
      481 aacggggcct tcaacgcacc ggtattgctg aatgtgggcc cgaacccgac ggcggtcgcc
      541 gccggagatc tcaaccgcga cgatcgcccc gacctcgcgg tcacctgtgc aggctccaac
      601 gacgttcgtg tgttccttgc aaacggcggg ggcacgttcg caagcgcgtc cgcgtacccg
      661 acgggtagcg cgccttccgc tgtcgcaatt gccgacctcg acaacgacgg ttggcccgat
      721 ctcgccgtca cgaacgagaa ctcgaatagc gtatccatcc ttaacggaac cggcagtggc
      781 gcgtttgcgg cgtcgccgaa tgccatcgca gtctcgccgc ggccactcgg catcacgacc
      841 ggccatcttg actcggacca cgtgctagat ctggtggtgg cgagcgccac gaataacatg
      901 ctgaccgtgc tcggcggcaa cggagatgga acattccgtt cgccggccgt cattcctgcc
      961 ggcgtgttca gccgcgcggt cgcgctggcg gatttcaacg ccgatagccg tctcgacgtc
     1021 gctgttggaa acatgaacgg caacgatgtc tggatcgtgc tgaaccagac cggcacgatt
     1081 gccgatctcg ccgtgagcgt atccaattac gcatcttcgt tgcggagcgg cgctcctgtc
     1141 tcgtacgagg cggtggtgtc gaacctcagc agtgcggatg tgtcggaact caatctgcaa
     1201 ctgaccgttc cgagcgcgct ggtttcgcct gcgttcacgc cttctgcggg cgcctacgac
     1261 cctgcgaccg gcgcctggac agggctgtct ctcggagggg gacagcaagc ggtcctgacg
     1321 attgcgggca cggtttcgga ggacgcggcc gggacaatca agctcgaagc catcgtgggc
     1381 ccgggcgccg gatataccga tccggtcgcc ggcaacaata cggcggaaga tttagacgtg
     1441 ttgatacgcg gcgacgctga tctccaggtc gcaatctcga atgggcggac cagtgtcgcg
     1501 gccggcgatg acgtcagcta cacgattacg gtgtccaatg tcggaccgat tgccgtgcag
     1561 ggagcgcacg tcacgacgct gctgccgggc gctctgcgga atgtcgcctg gagttgtacg
     1621 gcgagcgcgg gcgcggtctg cccaccggca ggaagcggag cgatcgacga actcctgtcg
     1681 ctgcctgtcg gcggctcggc gacgttcgtg ttagccgcct tcatcgacga gcgggtggcc
     1741 tcgggcaccc tggccgtgtc ggccagcgtt gccgctccct cggatgttat cgatcctgtg
     1801 gcggagaacg atgcagccac cgacgaggac gcgctgtccg gcgatgctcc gcccgttgct
     1861 gcgtctcaat cgctcacgct gtcggaagac ggctccgtcc aggtggaggt tgccgcaacc
     1921 gacgctgacg gagatgcact cacgttctcc gtcgtgacgc cgcccgcaca cggcgtgctg
     1981 tccggaacag ctccgtattt cacctatact ccggcggcca actatagcgg cgcggacacg
     2041 ttcgtcttcc aggcagacga cg
//
LOCUS       DASPLT010001510         1453 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_263991, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001510 DASPLT010000000
VERSION     DASPLT010001510.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1453)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1453)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1453
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_263991"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1453)
                     /gene="purL"
                     /locus_tag="VEA63_15440"
                     /gene_synonym="purI"
     CDS             complement(<1..>1453)
                     /gene="purL"
                     /locus_tag="VEA63_15440"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HYD85459.1"
                     /translation="PTAISPFPGAATGSGGEIRDEGATGRGAKPKAGLVGFTVSNLKL
                     PGAIQPWEKEHGKPNRIVSALDIMIEGPLGGAAFNNEFGRPAINGYFRTYEAEVPSAS
                     VPSSEFRVPSPQPETQNLEPGTAVSPQAELRGYHKPIMLAGGLGNIRAEHIAKGAINP
                     GDKLVVLGGPAMLIGLGGGAASSVASGQGSEDLDFASVQRDNAEMERRCQEVIDRCWA
                     LGTENPIAFIHDVGAGGLSNALPELVNDGGRGGKFNLRKIPNDEPGMTPLEIWCNESQ
                     ERYVLAIPARRIDTFAQLCERERCPFAIVGEATEEKRLVLEDPHFENKPIDLPLEVLL
                     GKPPRMHRTDRSLRRRLGELNLDDISLADAVRRVLSHPTVADKTFLISIGDRTVGGLI
                     CRDQMIGPWQVPVADCGVTAAAFDVYTGEALAMGERTPVAVNNAAASARLAVGEALTN
                     LAAAQIGDLGKVNLSANWMAAPALPGEGADLYAA"
BASE COUNT          230 a          421 c          529 g          273 t
ORIGIN      
        1 gcagcataga ggtcggcgcc ttcgccgggc agtgcgggcg ccgccatcca gttcgccgag
       61 agatttactt tgccgagatc gccgatttgc gcggcggcaa gattcgtgag cgcttcgccg
      121 acggcgaggc gcgcggaagc ggcggcattg ttgacggcga cgggcgtgcg ttcgcccatg
      181 gcgagggcct cgccggtgta gacgtcgaac gcggcggcgg tcacgccgca gtcggcgacg
      241 ggcacctgcc acggaccgat catttgatcg cggcagatga gtccgcccac ggtgcggtcg
      301 ccgatggaga tgaggaacgt tttgtcggcg accgtcggat gcgagagcac gcggcggacg
      361 gcatcggcga gcgagatgtc gtcgaggttc agttcaccga gccgacggcg aaggctacgg
      421 tccgtgcgat gcatccgcgg cggtttgccg aggagaactt cgagcgggag atcgatcggc
      481 ttgttttcga agtgcggatc ttcgaggacg aggcgctttt cctcggtcgc ttcgccgacg
      541 atcgcgaaag gacagcgctc gcgctcgcag agctgcgcga acgtgtcgat gcggcgggcg
      601 ggaatcgcga ggacgtagcg ttcctgcgac tcgttgcacc agatttcgag cggcgtcatg
      661 ccgggctcgt cgttcgggat tttgcgcagg ttgaatttcc cgccacgccc gccgtcgttg
      721 acgagctcgg ggagcgcgtt ggagaggccg ccggcaccga catcgtgaat gaaggcgatc
      781 gggttttcgg tgccgagtgc ccagcaacga tcgatgactt cctggcagcg gcgctccatc
      841 tcggcgttat cacgctggac gctggcgaaa tcgagatcct cgctgccttg tccgctggcg
      901 acggaactcg cggcgccgcc gccgaggccg atgagcatcg cgggtccgcc gaggacgacg
      961 agtttgtccc ccggattgat cgcgcctttt gcgatgtgtt cggcgcggat gttgcccaaa
     1021 ccgccggcca acatgatcgg tttgtggtag ccgcgcagtt ccgcctgcgg cgaaacggcg
     1081 gttccgggtt ccaggttctg ggtttcgggt tgaggactcg gaacacggaa ctctgaactc
     1141 ggaacggaag cggagggaac ctccgcttca tacgtgcgga agtagccgtt gatcgcgggg
     1201 cggccgaatt cgttgttgaa ggcggcgccg ccgagcgggc cttcgatcat gatgtcgagg
     1261 gcggagacga tccggttcgg tttgccgtgc tctttttccc acggctgaat cgcgccgggt
     1321 aatttaagat tggatacggt gaaaccgacc aaaccggctt tgggtttcgc accgcggccg
     1381 gtggcccctt cgtcgcggat ttcaccgcca gagccggtgg cggcgccggg gaagggcgag
     1441 atcgcggtcg ggt
//
LOCUS       DASPLT010001511         1043 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_202974, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001511 DASPLT010000000
VERSION     DASPLT010001511.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1043)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1043)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1043
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_202974"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..795
                     /locus_tag="VEA63_15445"
     CDS             <1..795
                     /locus_tag="VEA63_15445"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HYD85460.1"
                     /translation="AGARFLCSGGVLYAFLRWRGAPKPTARQWTASAIAGTFLLLGGN
                     GLVAWAEQFVPSGVTALLVGITPLFFVLTEWAWPGGTRPSLVTMAALLLGFAGVAWLA
                     APWENGAHEGVHVGGALAIIGAALSWAIGSIYSRHAKHSADPMLASALQMLTGGTVLT
                     FVALLHGDFSAIDFPAISLRSWTAFAYLTLFGSLVGFSTFVWLMKHSTPARVSTYAYV
                     NPVVAVLLGWWIADEVVTARTLTASVVIVSAVVIITLQRTRVPAQR"
BASE COUNT          148 a          347 c          307 g          241 t
ORIGIN      
        1 gcaggcgcgc gtttcctttg ctcgggcggc gtgctctatg catttctccg ctggcgcggc
       61 gcgcctaaac ccaccgctcg acaatggacc gcaagtgcca tcgcgggcac gttcctgctc
      121 ctcggcggca acggcctggt cgcatgggcc gagcagttcg tgccatctgg agtgacggcg
      181 ctcctcgtcg gcatcacgcc gctttttttc gttctcaccg aatgggcctg gcccggcgga
      241 acacgaccca gcttggtgac catggctgcg ctcttgctcg gtttcgccgg cgtggcgtgg
      301 ctggccgcgc cctgggaaaa cggcgctcac gagggtgtgc acgtcggcgg cgccctcgcg
      361 atcatcggcg ccgcactttc atgggcgatc ggctcgatct attcgcgcca cgcgaaacac
      421 agcgccgatc ccatgctcgc gtccgccttg caaatgctga ccggcggcac ggtgctgact
      481 ttcgttgcgt tgctgcatgg cgatttctcc gcgatcgatt tcccggccat ctcgctgcgt
      541 tcgtggaccg ctttcgcgta tctcacgctc ttcggttccc tcgtcggatt ttcgactttc
      601 gtttggctga tgaaacatag cacgcccgcg cgagtttcga cctacgccta cgtgaatccc
      661 gtcgtcgctg tccttctggg ttggtggatt gcggacgagg tcgtcaccgc gcgcacgttg
      721 accgcatcgg tcgtgatcgt gtccgccgtt gtaatcatca ccttgcagag aacacgcgtt
      781 cccgctcagc gctgacctag agataaggaa cggctgttga ccgtcgcacg agtcgctgga
      841 tttgcgacgg catgcctcgg aggcctgcgc agtggagttg agccccaagg ttttgcgggc
      901 gccctccagc ttcgcgccga agtttttcga aatcctcctt gccagacacc ccacatgcac
      961 taggtgaatc accctagttt atgaattttg gccgccatct ctttctgtcg cgcccctcaa
     1021 cgctccccgc attggctgcg ggc
//
LOCUS       DASPLT010001512         1047 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_283763, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001512 DASPLT010000000
VERSION     DASPLT010001512.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1047)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1047)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1047
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_283763"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1047
                     /locus_tag="VEA63_15450"
     CDS             <1..>1047
                     /locus_tag="VEA63_15450"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HYD85461.1"
                     /translation="ERMLGYTSTELVGRAKPDMFHVLSELKGRGAELSERIGREVAPG
                     FEAVVAMARGGEVDEREWTYVRKDGKRLPVLLTTTALRDREGEVTGFLCIAQDLTERK
                     RAEAALLASEQRLRQVLAHADCLVWEAGVRMTASDWNWRYTVYPSGLYRRLFGEGDTQ
                     QNAGLWYRFEIPEREELNRRSREALEQGRSGYVQEFRVPHNGGITWMRESVAIREVEP
                     GYFWLVGVAIDVTKQKDAEAVLQESEERFRNAFESAGIGMALVGLDGRWLRVNRTLCD
                     IVGYSEDQLLHLRFADITHPEDLETDRALTRRLLEGEARYYQIEERFLHRDGQTLWVR
                     LTASAVRDAAGTPL"
BASE COUNT          185 a          275 c          402 g          185 t
ORIGIN      
        1 cggagcgaat gctcggctac accagcacgg agctggtggg gcgggcgaag ccggacatgt
       61 tccatgttct ttcggagttg aaagggcgcg gtgcggaatt gagcgagcgc atcgggcgcg
      121 aggtggcgcc cggcttcgag gcggtcgtgg cgatggcgcg aggcggcgag gtggacgaac
      181 gcgaatggac ttacgtgcgg aaggatggga aacggctgcc ggtgctgctg acgacgacgg
      241 cgcttcgcga tcgcgaaggt gaagtgacgg gctttctctg tatcgcgcag gatttgaccg
      301 agcgaaaacg cgcggaagct gcgctcctcg cgagcgagca gcggctacgg caggtgctgg
      361 cgcatgcgga ctgccttgtg tgggaggcgg gcgtgcgcat gacggcgtcg gactggaact
      421 ggcgctacac cgtttatccc tcggggcttt atcgacggct ttttggcgaa ggcgataccc
      481 agcagaatgc ggggctgtgg tatcgcttcg agattcccga gcgcgaggaa ttgaatcgcc
      541 gcagccgcga ggcgctcgaa caaggccgta gcggttacgt gcaggaattt cgtgtgccgc
      601 acaacggggg catcacgtgg atgcgtgaat ccgtcgcgat tcgggaggtg gagccgggct
      661 atttctggct cgtcggcgtg gcgattgacg tgacgaagca gaaggatgcc gaggcagtgt
      721 tgcaggaaag cgaggagcgc ttccgcaacg cgttcgagtc ggcgggcatc ggcatggcgc
      781 tcgtggggct cgatgggcgc tggctgcgcg tgaaccggac gttgtgcgac atcgtgggat
      841 attccgagga tcagttgctg cacctgcgat ttgcggacat cacgcacccg gaggatctcg
      901 agaccgatcg ggcactgacg cgtcggctgc tcgaagggga ggcacgctac tatcagatcg
      961 aagaacgctt cctgcaccgc gacggccaga cgctttgggt caggttgacg gcgtccgcag
     1021 tgcgcgacgc ggcgggcacg ccgttgc
//
LOCUS       DASPLT010001513         1640 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_222548, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001513 DASPLT010000000
VERSION     DASPLT010001513.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1640)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1640)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1640
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_222548"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1640
                     /locus_tag="VEA63_15455"
     CDS             <1..>1640
                     /locus_tag="VEA63_15455"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HYD85462.1"
                     /translation="RANLELPSGRIEGELVELTVRTLSRLSTPEEFEDLIVKQSGDAL
                     VRLRDIGRAQLAALNERTLLRRDGVPMVGVVLRPQPGANYIEIADEFHRRVEQIKPEL
                     PDDIVVSYGFDITNFIRSSIIEVVETIFLAIVLVVAIIFLFLREWRSTFIPIVVIPIS
                     LIAAFFVMYIAGFSVNVLTLLALVLAIGLVVDDAIIVLENIYAKIEQGMDPLEAGLTG
                     TKEIFFAVIATTVALAAVFTPLLFMGGLTGQLFKEFGVVLAGAVVISAFVALTLTPML
                     STRLLKAGSHNGWFYRKSEPFYRRLTAGYRSSLAAFLRHRWIAFVILVGCGGASWWLF
                     QALPSELAPMEDRSSLRINATAAEGTSYETMAAFMSSLDEVLSREVPEIAEATAMTSP
                     GFGGAASVNSGFVRITLTPPAERERSQAEIATALRRAVRNLPGARISIQQEPTIGDRR
                     AGAPVQFVLQAARLEELEKVLPAFLDRAAADPTFTFVDTNLRFNKPELRIDIDRNRAQ
                     ALGVSVRDVAETLQLTLSEQRYGYFILDDEQYQVIGQL"
BASE COUNT          262 a          448 c          595 g          335 t
ORIGIN      
        1 agcgcgcgaa cctcgagctg ccgtcggggc ggatcgaggg cgaactggtc gagttgacgg
       61 tgcggacatt gagccggctt tcgacgccgg aggagttcga ggatctgatc gtcaagcaat
      121 cgggcgatgc gctggtgcga ttgcgcgaca tcggtcgggc gcagctggct gcgctgaacg
      181 agcgcacgct gttacggcgc gacggtgtgc cgatggtggg cgtggtgctg cggccgcaac
      241 cgggcgcgaa ctacatcgag atcgcggacg aatttcatcg ccgggtcgag cagatcaagc
      301 cggagctgcc ggacgatatc gtggtgagct acggtttcga catcacgaac ttcattcgct
      361 cctcgatcat cgaggtggtg gaaaccatct tcctcgcgat cgtgctggtg gtcgcgatca
      421 tcttcctgtt tttgcgcgag tggaggtcga cgttcatccc catcgtcgtg atcccgattt
      481 cgctgatcgc ggcgtttttc gtgatgtata tcgcgggttt ttcggtgaac gtgctgacgc
      541 tgctggcctt ggttctcgcg atcgggctgg tggtcgatga cgcgatcatt gtgctggaga
      601 atatctacgc gaagatcgag cagggcatgg atccgctcga ggcagggctg accgggacga
      661 aggaaatttt ctttgcggtg atcgcgacga cggtggcgct tgcggcggtg ttcacgccgt
      721 tgttgttcat gggcggattg acgggccagt tgttcaagga attcggcgtg gtgctcgcgg
      781 gcgcggtggt gatctcggcg tttgtcgcgc tgacgttgac gccgatgttg agcacacggc
      841 tgctcaaagc gggcagtcac aacggctggt tctaccgcaa gtcggaaccg ttctaccggc
      901 gcctgacggc gggatatcgc tcgagcttgg cggcatttct gcggcatcgc tggatcgcgt
      961 tcgtcatcct cgtgggatgc ggcggggcgt catggtggtt gttccaggcg ctgcccagcg
     1021 aacttgcgcc gatggaggat cgtagcagcc tgcggatcaa tgcgacggcg gcggagggca
     1081 cgtcgtatga aacgatggcg gcgttcatga gcagtttgga cgaggtgctg agccgcgagg
     1141 tgccggagat cgcggaggcg acagcgatga cttcgcccgg ttttggcggc gcggcctcgg
     1201 tgaattccgg tttcgtgcga atcacgctga cgcctccggc cgagcgggag cgcagccagg
     1261 cggaaatcgc gacggcgctg cggcgcgcgg tgcgcaatct gccgggcgcg cgtatttcca
     1321 tccagcagga accgacgatc ggcgatcgcc gcgcgggcgc gccggtgcag ttcgtgttac
     1381 aggcggcgcg gctcgaggag ttggagaagg tcttgccggc tttcctcgac cgcgcggcgg
     1441 cggatccgac gttcacgttc gtcgacacga acctgcgctt caacaagccg gagctgcgca
     1501 tcgacatcga ccgcaatcgc gcgcaggcgc tgggcgtgtc ggtgcgcgac gtggcggaga
     1561 cgctgcagct cacgttgagc gagcagcgct acggctactt cattctcgac gacgagcagt
     1621 atcaggtgat cgggcagttg
//
LOCUS       DASPLT010001514         3184 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_305563, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001514 DASPLT010000000
VERSION     DASPLT010001514.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 3184)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3184)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3184
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_305563"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(142..>3184)
                     /locus_tag="VEA63_15460"
     CDS             complement(142..>3184)
                     /locus_tag="VEA63_15460"
                     /inference="COORDINATES: protein motif:HMM:NF040048.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF6298 domain-containing protein"
                     /protein_id="HYD85463.1"
                     /translation="PAAARLAYTADVAGNRVIDFSHAGYEGGGKPIPFVPAKIVVEPS
                     ADGSSDRLRIQAALDFVAAMPRDPATGFRGAVLLRPGRYRIDSQLRLNASGVVLRGSG
                     QGGTAHDTVLVATGTSRRTLIEIGGTDNRIELPDTRRAIRDAYVPVDATSFNVGSTAH
                     LSVGDRIIVHRPSTAEWISLLGMDTFPGWRAEGRLHWKPGSRDLEWDRTLTAIEGDRI
                     TIDAPLTTAIDRAYGGGSIYRYDFPGRIDHVGIENLRCVSEHLNDTAPAHPLAAPAVN
                     LEEHAWVCVSLDNVENAWIRQLTARHFVSYVVKTGAASRAITIEDCIAEDPVSEIASY
                     RRRVFSIGGQLTLVQRCESMQGRRDFTTGFTAAGPNVFLHCTARDALDYSGPVESWAS
                     GVLYDNVTIRGNALRLLNHGIDGQGAGWAAANSILWNCDATDIQVQSAPGAPNQAYGC
                     KGDVTDDSLIYDPARIPYRGFVRGMPTQPASLYLAQLAERRGAETTARTEISLAADHA
                     TLLKPSDISGELPAPAPHPLRIENARFTIDGHPAFTETVNYSWYQAQMPPKLARTYGP
                     ALTRFAPGESSPGLTDNLAELAAAMPPRAAFVQHYGLWYDRRRVNHNFSGAPEHRDAN
                     VWAPFMEMPWARSAQGRDWNGLSKYDLARFNPWYFSRLKEFADLADARGLVLYNYFYF
                     QHALQETRAHYVDFPWRPVNCIQKTDLPDENPAASAFYDIAHPVRRELHRLYLRHTLD
                     VLGANTNVVFGLDREYTGPLSFVEFVLDTIAEWQSEHDRKIHLALEIPKDQLDALLAD
                     PQRAPLISAIAFHHWFYRADGSLYAAIGGINKAPREQSPGIVTEADLATLRTQITNPV
                     HFGANIVSSPEFQRLAATVRASTPAMRYRALREYRDAFPHLVILQVDDDFPALTAAIE
                     KQIPSTLRAQTRPAALLRNHRDSAWCMAAPGRAYLIYSMAGEAVDLDLSADTGVFNLT
                     WLDSDTGELVAASAPITAGAALTLTPPETNTHRPWIAWLVP"
BASE COUNT          552 a          779 c         1288 g          565 t
ORIGIN      
        1 acggccgccc tcttcggtga gggcggccaa gtcctcacca tgactggaat cgcctgctgc
       61 gacgagctct tcggcccatt ctcgcaggcg cttccagtca tggtctctcc ctctcatcga
      121 ggggacatac aaggcctgcc gttacgggac gagccaggcg atccaagggc ggtgtgtgtt
      181 ggtttcagga ggagtgagcg tgagcgcggc gccggcggtg atgggcgcgg aggcggcgac
      241 gagttcgccg gtgtcggagt cgagccaggt gaggttgaaa acgccggtgt cggccgagag
      301 atccaggtca acggcttcgc cggccatcga gtagatgaga tacgcgcgtc cgggagcggc
      361 catgcaccag gcggagtcgc gatgattgcg gaggagcgcg gcggggcgag tttgagcgcg
      421 cagcgtggaa ggaatttgtt tttcgatggc ggcggtgagc gcggggaaat cgtcgtctac
      481 ttgaaggatg acaaggtggg ggaaggcatc gcggtattcg cggagcgcgc ggtagcgcat
      541 ggcgggcgtg ctggcgcgga cggtggcggc gaggcgttgg aattcgggcg agctgacgat
      601 gttggcgccg aagtgaaccg ggttcgtgat ctgcgtgcga agcgtggcga gatcggcttc
      661 ggtgacgatg ccgggagatt gttcgcgcgg ggctttgttg atgcctccga tcgcggcgta
      721 gagcgagccg tcggcgcggt agaaccaatg atgaaacgcg atcgcggaga tcagaggagc
      781 gcgttgcgga tcggcgagga gggcgtcgag ctggtctttc ggaatttcga gcgcgaggtg
      841 aattttgcgg tcatgctcgg attgccattc ggcgatcgtg tcgaggacga attcgacgaa
      901 ggagagcggg ccggtgtatt cgcggtcgag gccgaagacg acgttcgtgt tcgcgccgag
      961 gacatcgagc gtgtgtcgga ggtaaaggcg gtgaagctcg cggcgcacgg ggtgcgcgat
     1021 gtcgtagaag gcggaggcgg cgggattctc gtcggggaga tccgttttct ggatacagtt
     1081 gaccggccgc cacggaaaat cgacgtagtg ggcgcgggtt tcctgcagag cgtgttgaaa
     1141 atagaagtag ttgtagagga cgagaccgcg ggcgtcggcg aggtcggcga attccttgag
     1201 tcgcgaaaaa taccatgggt tgaaacgggc gaggtcgtat ttcgagaggc cgttccaatc
     1261 gcgaccctgg gcgctgcgag cccagggcat ctccatgaag ggagcccaga cgttggcgtc
     1321 gcggtgctcg ggtgcgccgg agaaattatg gttaacgcgg cggcggtcat accagagccc
     1381 gtaatgttga acgaaggcgg cgcgcggcgg catcgcggcg gcgagctcgg cgagattgtc
     1441 ggtgagaccg gggctgcttt cgccgggggc gaaacgcgtg agcgcggggc cgtaggttcg
     1501 cgcgagtttc ggcggcatct gcgcttgata ccacgagtag ttcacggttt cggtgaacgc
     1561 gggatggccg tcgatggtga agcgggcgtt ttcgatgcga aggggatgag gagcaggcgc
     1621 agggagttcg ccggaaatgt ccgatggctt gagcaaagtg gcgtggtcgg cggcgagcga
     1681 gatttccgtt cgcgcagtgg tttcggctcc gcggcgttcg gcgagttggg cgagataaag
     1741 gctcgcgggt tgtgtcggca tgccgcgaac aaagccgcga tagggaatgc gggcgggatc
     1801 gtagatgagg ctatcgtcgg tgacgtcgcc tttgcagccg taggcttggt ttggggcgcc
     1861 gggtgcgctt tggacctgga tgtcggtcgc gtcgcagttc caaagaatgg agttggcggc
     1921 agcccagccg gcgccttgtc cgtcgatgcc gtggttgagg agacggaggg cgttgccgcg
     1981 gatcgtgacg ttatcgtaga gcacgccgga ggcccagctt tcgaccgggc cgctgtagtc
     2041 gagggcgtcg cgcgcggtgc agtgaaggaa gacgttcggg ccggcggcgg tgaagccggt
     2101 ggtgaaatcg cggcggccct gcatcgattc gcagcgctgg acgagggtga gttgtccgcc
     2161 gatggaaaag acgcggcggc gatagctggc gatttcggag acggggtctt cggcgatgca
     2221 atcttcgatg gtgatcgcgc gggaagcggc gccggttttg acgacatagc tgacgaagtg
     2281 gcgggcggtg agctggcgaa tccaggcatt ttcaacgtta tcgagcgaga cgcaaaccca
     2341 cgcgtgttcc tcgagattga cggcgggtgc ggcgagcgga tgcgcgggcg cggtgtcgtt
     2401 caggtgctcg gaaacgcagc ggagattctc gatgccgacg tgatcgatgc ggccggggaa
     2461 atcgtagcga taaatggagc cgccgccgta agcgcgatcg atcgcggtgg tgagcggggc
     2521 gtcgatggtg atgcggtcgc cttcgatcgc ggtgagggtg cggtcccact cgaggtcgcg
     2581 cgaaccgggt ttccaatgga ggcgaccttc agcgcgccaa ccgggaaacg tgtccatgcc
     2641 gaggagcgaa atccattcgg cggtgctggg gcggtgaacg atgatgcggt cgccgacgga
     2701 gaggtgggcg gtggaaccga cgttgaagga agtggcgtcg acgggaacgt aggcgtcgcg
     2761 gatcgcgcga cgtgtatccg gaagttcgat gcggttgtcg gtgccgccga tttcgatcag
     2821 cgtgcggcgg ctggtgccgg tggcaacgag aacggtgtcg tgggcggtgc cgccctggcc
     2881 ggagccgcgg agaacgacgc cggaggcgtt gagccggagt tgggagtcga tgcgatagcg
     2941 gccgggtcgg aggaggacag cgccacggaa accggtggcg gggtcgcggg gcatggcagc
     3001 gacgaaatcg agtgcggcct ggatgcgcag gcgatcggat gagccgtccg cagacggctc
     3061 gacaacgatt ttcgcaggaa cgaagggaat gggtttgccg ccgccttcgt agccggcgtg
     3121 cgagaagtcg atgacgcggt tgccggcaac atcggcggtg taggcgaggc gggcggcggc
     3181 gggg
//
LOCUS       DASPLT010001515         1527 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_182824, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001515 DASPLT010000000
VERSION     DASPLT010001515.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1527)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1527)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1527
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_182824"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..244)
                     /locus_tag="VEA63_15465"
     CDS             complement(<1..244)
                     /locus_tag="VEA63_15465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD85464.1"
                     /translation="MKSPRFLASLLFAGLSSLTPMFAAEPIKLATPSASAEAVGLFEY
                     LLQISGKHTLSGQHCVPLVGSTRLPGVHKALGHYPAV"
     gene            complement(601..1191)
                     /locus_tag="VEA63_15470"
     CDS             complement(601..1191)
                     /locus_tag="VEA63_15470"
                     /inference="COORDINATES: protein motif:HMM:TIGR01382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HYD85465.1"
                     /translation="MKQSITGKKVAILVTDGFEQSELEKPRAALDKAGAQTVIVSPKR
                     DSVRGWDKDDFGESVKVDVPLNKASPADYDALLLPGGVMNPDSLRTIPEAVQFVTHFF
                     EAGKPVAAICHGPQLLIEADVVRGRRLTSYPSIKTDLRNAGAEWVDEAVVTDQGLVTS
                     RRPADIPEFNEKMIEEIGEGYHAGQNRSTAANMSHA"
     gene            complement(1188..>1527)
                     /locus_tag="VEA63_15475"
     CDS             complement(1188..>1527)
                     /locus_tag="VEA63_15475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012372863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD85466.1"
                     /translation="GGELRDVKLHSPLSRPRAKGRDVYVYFDNDVKTHAPFDAMNLAH
                     RLGLGPAAPEGPEPGTVKFVPLRTGWPGWAGSDRRLQKVETRRKTPAARATSGKKERN
                     QKRTTQTSKT"
BASE COUNT          263 a          533 c          423 g          308 t
ORIGIN      
        1 acaccgccgg ataatgcccc aacgccttgt gcacgcccgg cagccgcgtc gatcccacca
       61 acggcacgca atgctgaccg ctcagcgtgt gcttccccga gatctgcaga agatattcga
      121 acaagccgac ggcttccgcc gaggccgatg gcgtcgccaa cttgatcggc tccgccgcga
      181 acatcggcgt cagcgacgag aggccggcga acaggagcga agcaaggaaa cggggcgatt
      241 tcatcgcgcg cgaatctgcg cccgcggttt tccgacacaa gcccgcgctc tcgaaatcaa
      301 ggtcgagccc accgtcccgt cgggctttct agagcagagc actcaaaacc gtcgccgcaa
      361 aaaggcacaa aaggcgcaaa gatggcttga gggctcacct gttcttgcgc cttttgcgcc
      421 ttcttgcggc caaaggtccg gctatagaat tttcccaccg ttcttgcgaa cacgctaagc
      481 ccgcgccggg tttcatccgg cgcgggccct tgttcagcgt tcaaccctct acccactcca
      541 acacggatcg cgaggcgctc gccaggcgtg cgactctcgc gacccgggaa atcgttccgc
      601 tcaagcgtgg ctcatgttcg ctgcagtcga tcgattttgt ccggcgtgat aaccctcgcc
      661 gatctcctcg atcatcttct cgttgaactc cgggatgtcg gccggtcgcc ggctcgtcac
      721 caggccttga tcggtcacca ccgcttcgtc cacccactcg gcgcccgcat tgcggaggtc
      781 cgtcttgatc gagggatacg acgtcaaccg ccgcccgcgc accacgtccg cttcgatcag
      841 caactgcggt ccgtgacaaa tcgccgccac cggttttccc gcttcgaaga aatgcgtcac
      901 gaactgcacc gcctcgggaa tcgtgcgcag cgaatccgga ttcatcacgc cacccggcag
      961 cagcagcgca tcgtagtccg ccggcgacgc tttgttcaac ggcacgtcga ctttcacgga
     1021 ctcgccgaaa tcgtccttat cccagccgcg caccgaatcg cgtttcggcg aaacgatcac
     1081 ggtttgcgcc ccggctttgt cgagcgcggc gcgcggtttc tcgagttcgc tttgctcgaa
     1141 tccgtccgtg acgaggatcg ccactttttt tccagtaatg ctttgtttca tgttttagag
     1201 gtttgagttg ttcgtttctg gtttcgttct ttctttccgg aagtcgcgcg cgccgccggc
     1261 gtttttcgcc gtgtctccac tttctggaga cgccggtccg aacccgccca gccgggccag
     1321 cccgtgcgca acggcacgaa cttcaccgtg ccgggctccg gcccttccgg cgccgccgga
     1381 ccaaggccca ggcgatgcgc gaggttcatc gcgtcgaacg gcgcgtgcgt cttcacgtcg
     1441 ttgtcgaaat acacatacac gtcgcgcccc ttcgctcgcg gccggctcag cggcgaatgc
     1501 aactttacgt cgcgcagctc gccgccc
//
LOCUS       DASPLT010001516         1815 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_182833, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001516 DASPLT010000000
VERSION     DASPLT010001516.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1815)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1815)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1815
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_182833"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1198)
                     /locus_tag="VEA63_15480"
     CDS             complement(<1..1198)
                     /locus_tag="VEA63_15480"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007359518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HYD85467.1"
                     /translation="MAARTPRLRPADCRQANPEEQIMLKDPSKKYRAFPPVDLADRTW
                     PSKTITKAPVWCSSDLRDGNQALIEPMNGERKRRMFDLLVQVGFKEIEVAFPSASQTD
                     FDFVRHLIESNAIPDDVTIQVLTQAREDLIRRTFESLRGAKRAIVHLYNATSPLFRRV
                     VFNTDEAGVKDIAVRHAKLFVELAAQQPETEFRFEYSPETFTATELPVALDVCNAVLD
                     VWKPTRDRKAIINLPATVEVATPNVYADQIEWMCRHIKYRDSVMISLHPHNDRGCGIA
                     AAELGQMAGADRVEGCLFGNGERTGNVDIVTLALNLYTQGIDPQLDFSDMNAVARTVE
                     HCNQLPIHPRHPYVGDLVFTAFSGSHQDAIKKGFAAQRADQPWEIPYLPLDPADVGRT
                     YESVIRV"
     gene            1654..>1815
                     /locus_tag="VEA63_15485"
     CDS             1654..>1815
                     /locus_tag="VEA63_15485"
                     /inference="COORDINATES: protein motif:HMM:NF020928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tex-like N-terminal domain-containing protein"
                     /protein_id="HYD85468.1"
                     /translation="MLPPIEHRIAAELSVKPQQVAAAVALLDEGATVPFVARYRKEVT
                     GGLDDTQLRT"
BASE COUNT          333 a          551 c          599 g          332 t
ORIGIN      
        1 tcacgcgaat gaccgattcg taggtacggc ccacgtccgc cgggtcgagc ggcagatagg
       61 gaatttccca tggctgatcc gctctctgcg cggcaaagcc cttcttgatt gcatcctgat
      121 gcgaacccga gaaggcggtg aacacgaggt cgccgacata gggatggcgc ggatggatcg
      181 gcaactgatt gcaatgctcc accgtgcgcg cgaccgcgtt catgtccgag aagtcgagct
      241 gcggatcgat gccttgcgtg tagagattca aagcgagggt cacgatatcg acgttgccgg
      301 tgcgctcgcc gttgccgaac aggcagcctt ccacgcggtc ggcgcccgcc atctggccga
      361 gttccgccgc cgcgatgccg cagccgcggt cgttgtgcgg atgcaggctg atcatcacgc
      421 tgtcgcggta cttgatatgc cggcacatcc attcgatctg gtcggcatac acgttcggcg
      481 tcgcgacttc gaccgtcgct ggcagattga tgatcgcctt gcgatcgcgc gtgggtttcc
      541 acacatcgag cacggcgttg cacacgtcga gcgcgacagg cagttcggtc gctgtgaagg
      601 tctccgggct gtactcgaaa cggaattcgg tttcgggctg ctgcgccgcc agctccacga
      661 acagcttcgc atggcgcacg gcgatgtcct tcacgcccgc ttcatcggtg ttgaacacga
      721 cgcggcggaa cagcggcgag gtcgcgttat agagatgcac gatggcgcgc ttcgcgccgc
      781 gcagcgattc gaaggtgcgg cgaatgagat cttcgcgcgc ctgcgtcagg acctggatgg
      841 tgacgtcgtc gggaatcgca ttcgactcga tcaggtggcg cacgaagtcg aagtcggtct
      901 gcgaggcgga cggaaacgcg acttcgattt ccttgaagcc gacctgcacc agcagatcga
      961 acatgcggcg cttgcgctcg ccgttcatgg gctcgatcag cgcctgattg ccgtcgcgca
     1021 gatcggagga gcaccacacg ggcgccttgg tgatcgtctt cgacggccag gtgcgatcgg
     1081 ccagatcgac cggcgggaag gcgcggtatt tcttcgacgg atctttcaac atgatctgtt
     1141 cctcgggatt cgcttgacga cagtcggccg gacgtaggcg gggcgtgcgg gcggccacgg
     1201 attcaaggcc cgcaagccgt tgcgcagtcg cagcgatacg aaggaataca gggcggcaat
     1261 caggttcatg tgatctccag tgcgtggaaa tgcgccgaat ccgccgggct ttcgctccgg
     1321 cgacgggtac tgctcgaatt gggggtgtgg gatttatctg cgcgcggggc gcagcagcag
     1381 cagaccgagc gccagcgaga gggcgccgaa agcgaaatac gcggtctgga gagacgagat
     1441 catggcggca ctgtaacgga cggttccggg aagcgtcaag gcaggcacag gaaagtcgtc
     1501 acgccgaaag catccattcc ggacgccgcc gcgtcgtgcc gcgggaaatg acgcgcagca
     1561 tcgcgcattt cacggtttcg cgtctcgcgc aagcttgtca ggccggggtc gagcctgcat
     1621 agtgcgcggc gtttcacgtc tcccgcagtc ctcatgcttc cccccatcga acaccgcatc
     1681 gccgcagaac tttccgtcaa acctcagcaa gtcgccgccg cagtggctct cctggacgag
     1741 ggtgccaccg tgcccttcgt cgcccgctac cgcaaggaag tgacgggcgg actggacgac
     1801 acgcaactgc gcacg
//
LOCUS       DASPLT010001517         2116 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_99204, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001517 DASPLT010000000
VERSION     DASPLT010001517.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 2116)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2116)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2116
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_99204"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            233..1852
                     /locus_tag="VEA63_15490"
     CDS             233..1852
                     /locus_tag="VEA63_15490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fucose isomerase"
                     /protein_id="HYD85469.1"
                     /translation="MSHSPVLLVANGDLRPSANEKCWPAQAAMEKQLAAAVARFGRKL
                     VRAHPYKPKQKHGFISSQKEGMAVFAKIDPDAPLIVAESVWQYSHHVLAGLISHRGPI
                     LTVANWSGTWPGLVGMLNLNGSLTKAGVNYSTLWSEDFTDELFRDGLATWLATGSVQH
                     PTPHVRSFDRANVPARATKLARRLADELRTSKAIMGVFDEGCMGMFNAIVPDELLFPT
                     GVYKERLSQSSLYYAATQVSEVEARAVYAWLKKKGMTFHLGTDEATELTENQILWQCR
                     TYIAALRLADEFGCDTIGIQYQQGLKDLLPASDLVEGLLNNADRPPVKNAAGRVIRPG
                     EPLPHFNEVDECAGLDGLLTFRVHRALNQPVENTLHDLRWGDFDRSGTTKDYVWVFLI
                     SGSAPPAHHVNGYKGTDSHRQPPMYFRLGGGTVRGVAKPGEIVWSRVFVEGGKLKMDL
                     GRAKVLSLPPEETERRWRETTPQWPIMHAVTYGVSRDQMMARHKANHIQVAYAKSARE
                     ADLALYAKAALARELGLEVAFCGTRADGTLF"
     gene            1891..>2116
                     /locus_tag="VEA63_15495"
     CDS             1891..>2116
                     /locus_tag="VEA63_15495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase"
                     /protein_id="HYD85470.1"
                     /translation="MKSPAKPPAPLNTLLALAHDLGREDRQLAILGEGNVSTRLSFDT
                     FLVKASGSNLATLDAAGVTACRSSNLLDLLE"
BASE COUNT          392 a          793 c          559 g          372 t
ORIGIN      
        1 cgggcccgtc gcccgcgggc cgagctctag aaaaaaataa tcgcccgagc cgacttccgg
       61 cgcgagcagc gcctgctcct gccgggcgga aacggacttc atccggcgag ttcaggtcga
      121 acgcacgttt tgtccatccc atttccagca ttgtctattc tgccggtccg cgttctgtgc
      181 gaaattatcc cgtcgcgttt cccgcccttt caacgccttc gcgcaaatat ccatgtcgca
      241 ctctcctgtc ctcctcgtcg ccaacggcga ccttcgcccg tccgccaacg aaaaatgctg
      301 gcccgcccag gccgcgatgg aaaaacagct cgccgccgcc gtcgcccgct tcggccgcaa
      361 actcgttcgc gcgcatcctt acaagccgaa gcaaaaacac ggtttcatca gctcgcaaaa
      421 agagggcatg gccgtgttcg cgaagatcga tcccgacgcc ccgctcatcg tcgccgaaag
      481 cgtctggcaa tactcgcatc acgtgctcgc cggcctcatc tcgcatcgcg gcccgatcct
      541 caccgtcgcg aattggtccg gcacctggcc gggcctcgtc ggcatgctca acttgaacgg
      601 ctcgctcacc aaggccggcg tgaattactc gacgttgtgg agcgaggatt tcaccgacga
      661 gttgttccgc gacggcctcg cgacgtggct cgccacgggc agcgtgcagc accccacgcc
      721 tcacgttcgc tcattcgatc gcgccaacgt tccggcgcgc gcgacgaaac tcgcccgccg
      781 cctcgccgac gaactccgca cgagcaaggc catcatgggc gtcttcgacg agggttgcat
      841 gggcatgttc aacgcgatcg ttcccgacga gctgcttttc cccaccggcg tttataagga
      901 gcgcctctcg caatcctccc tctattacgc cgcgacacag gtcagcgaag tcgaggcgcg
      961 cgctgtctac gcctggctca aaaagaaagg catgaccttt cacctcggca ccgacgaagc
     1021 caccgaactc accgaaaacc agattctctg gcagtgtcgc acctacatcg ccgcgctgcg
     1081 cctcgccgac gagttcggct gcgacaccat cggcatccaa tatcaacaag gcctcaaaga
     1141 tcttctccct gcctccgatc tcgtcgaagg cttgctcaac aacgccgacc gtccgcccgt
     1201 gaaaaacgcc gccggccgcg tcattcgccc gggcgagccg ctgccgcatt tcaacgaagt
     1261 cgacgaatgc gccggccttg acggcctcct gacgtttcgc gttcaccgcg ccctcaacca
     1321 gccggtcgaa aacacgctgc acgatctgcg ctggggtgac ttcgatcgca gcggcaccac
     1381 gaaggactac gtttgggtgt ttctcatctc cggcagcgcg ccgcccgcgc atcatgttaa
     1441 cggatacaaa ggcaccgact cgcatcgcca gccgccgatg tatttccggc tcggcggcgg
     1501 caccgtgcgt ggcgtcgcca aacccggcga gatcgtgtgg agccgcgttt ttgtcgaagg
     1561 cggcaaactc aagatggacc tcggccgcgc caaagttctc tcgctgccgc ccgaggaaac
     1621 ggaacgccgc tggcgcgaaa ccacgccgca atggccgatc atgcacgccg tcacctacgg
     1681 cgtctcgcgc gaccagatga tggcgcgcca caaagccaac cacatccagg tcgcctacgc
     1741 gaagagcgcc cgcgaggccg acctcgcgct ctacgccaaa gccgcgctcg cccgcgaact
     1801 cggcctcgaa gtcgccttct gcggcacccg cgccgacggc acgctattct aaccgtagcg
     1861 gcaggcctcc gtgcctgcct gccttctccc ttgaaatccc ccgccaaacc tcccgccccg
     1921 ctcaacaccc ttctcgccct cgcccacgat ctcggacgcg aagatcgcca actcgcgatc
     1981 ctcggcgaag gcaatgtctc cacccggctc tcgttcgaca cgtttctcgt caaagccagc
     2041 ggctccaatc ttgcgaccct cgacgccgcc ggagtcaccg cctgccgctc gtccaatctg
     2101 ctcgatttgc tcgagc
//
LOCUS       DASPLT010001518         1166 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_78956, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001518 DASPLT010000000
VERSION     DASPLT010001518.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1166)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1166)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1166
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_78956"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..815
                     /locus_tag="VEA63_15500"
     CDS             <1..815
                     /locus_tag="VEA63_15500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD85471.1"
                     /translation="CLNTLLATYRAHPEFARTSWLWRNIFAGLKARRCELAATGVVAP
                     DGSIGAVVLEPKLRACLEHANIAQLLTPRQREESRFAAVQNGNVKRRIFQRSRAMGWK
                     PGFAAESAQLSQQTGKHVAEVLMTIGRLGSRAQLTANLLSLGISAVMLFALPDLRNIL
                     VPPRPPAVVAPSSSSPYALWVSIDTREPGCFRAVFESSAWANRLADFEWKSSDAPARA
                     EIPLRRLTQRTTRDQGHGTVWVERRRRFLHREYFPGERVGHYTMPYLNRLAP"
     gene            812..>1166
                     /locus_tag="VEA63_15505"
     CDS             812..>1166
                     /locus_tag="VEA63_15505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD85472.1"
                     /translation="MSECRTIVVRNVAFALLAFVIAGCANRDDVQVTLVSHVPTGESG
                     LRVEVHAQVAGAQEGLSYKWYAVAGECEPQESDAPRTVFTFANNIPIDRVVVEVWREG
                     QRVARGDIDLKFDAEM"
BASE COUNT          190 a          354 c          388 g          234 t
ORIGIN      
        1 tttgtctcaa caccttgctc gcgacgtatc gcgcgcatcc cgaattcgcg cggacatcgt
       61 ggctctggcg aaatatcttc gctggactga aggctcgtcg ttgcgagttg gcggccacgg
      121 gcgtcgtcgc gcctgacgga tcgatcggcg cggttgtgct ggagccaaag ctgcgcgcct
      181 gtctcgagca cgcgaacatc gcccaacttt tgacgccacg tcagcgggag gagtcgcgtt
      241 tcgcggcggt gcagaacggc aacgtgaagc gccgaatttt tcagcgaagc cgcgccatgg
      301 gctggaagcc ggggtttgcc gccgagagcg cgcaactttc ccaacagacc ggcaagcacg
      361 ttgccgaggt gttgatgacg attggtcgtc tcggcagccg cgcccagctc acggcgaacc
      421 tgctctcgct cggcatttcg gcggtgatgc ttttcgcttt gcccgatctg cgaaacatcc
      481 tggtgcctcc tcgtccgccg gcagtggtcg cgccctcttc gtcgtcaccg tatgcgctgt
      541 gggtgagcat cgatacgcgc gagccagggt gttttcgcgc ggtgttcgaa tcgtcggcgt
      601 gggccaatcg cctcgccgat ttcgagtgga aaagcagcga cgcgcccgct cgcgcggaga
      661 tcccgcttcg acgtttgacg cagaggacca cccgcgacca ggggcatggg acggtgtggg
      721 tcgagcggcg gcgtcgcttt ctccatcgcg agtattttcc gggagaacgc gtcggtcact
      781 acaccatgcc ttacctgaac cgccttgccc catgagtgaa tgccgcacca tcgtcgtccg
      841 caatgtagcg ttcgcgctac tcgctttcgt gatcgccggg tgcgcgaacc gcgacgacgt
      901 gcaggtgacg cttgtctccc acgttcccac gggcgaaagc ggattgcggg tggaggtgca
      961 tgcgcaggtc gccggcgcgc aagagggtct ttcctataaa tggtatgcgg tcgcggggga
     1021 atgcgagccg caggagtccg acgcgccacg cacggtcttc acgtttgcga acaatatccc
     1081 catcgaccgg gtggtcgtgg aggtttggcg tgaagggcag cgggtcgctc gcggcgacat
     1141 cgatctgaaa ttcgatgcgg agatgg
//
LOCUS       DASPLT010001519         1685 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_57724, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001519 DASPLT010000000
VERSION     DASPLT010001519.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1685)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1685)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1685
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_57724"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(695..1375)
                     /locus_tag="VEA63_15510"
     CDS             complement(695..1375)
                     /locus_tag="VEA63_15510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD85473.1"
                     /translation="MAWRIDEAVIRGEIDNRVRGRVTGRIWFVGKGAPVELDLAGDAW
                     RDLAGRRLEFVNPQPKALEMLERFAARQTGVIGDCTASRKVKVPDIPMDQIGEYYAAK
                     KKWTWHWGNSLYLEWFSLTNGRVVIESVSYQMTVSPDIAWEMTPAEEEQQRRANAGRW
                     GISWNAWDRRWRTRRRAMSRVSRRRTRRKTQTTRRAQTTPSSRMRTPMRMTKHYRKAT
                     RRRTATSR"
     gene            1480..>1685
                     /locus_tag="VEA63_15515"
     CDS             1480..>1685
                     /locus_tag="VEA63_15515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2959 family protein"
                     /protein_id="HYD85474.1"
                     /translation="MQVQRARHRAAALAACAFALLATGCSSTYYNAMEKIGFAKREIL
                     VDRVEDTRDAQNDAKEQFASALEQ"
BASE COUNT          294 a          640 c          412 g          339 t
ORIGIN      
        1 gattcacgcc gcgatgtatg ccttcttccg acctgtcacc cgcatccgcg ctcgccgcgt
       61 gaccccaccc gacgcgcttc tccgctctcg cggttttacc ctgacctccc gctgcacctt
      121 cgaactcggc ctcctccaat ccgacctctg gtcacgcggg tgaaccggcc gtcgatcagc
      181 ttggccgcaa aaacgcacaa ggcgcagaaa aaaattggcc gatcagcctt tcacttctcc
      241 gaccgagccc aagcctgtta cacatcgcgg ccaagccgca accaaaccac agaattcaat
      301 cctgtttaca gaaggaaacg aagagtcgca cgacaactcg gtgctctttg ttcacgtcgt
      361 taccttctgt tcaattccgg atccgggttt ttccaacggc cgatttcttg cgcggagaac
      421 aagattttca aaccatgtaa cacagagtgc acgcctacga cacggaggcc accgagtggg
      481 caaggagtct ggctccgtgc actctgtgtc cgagctcagt gccctctgtg taacaaatct
      541 tcggctctgc attaatccgg cgcctcgcct cgctcacgcc tgcgacgctc cagttcctca
      601 tcgaggatct gctcgaaatt cgccgcgtca ccttctcgtt ccatccgcgc gttgatccgg
      661 tcgagtagaa gttcgttctc cgcgtccgct tcggtcaacg gcttgtcgct gtcctccgtc
      721 tcgtcgcttt ccgatagtgc ttcgtcattc gcatcggcgt tcgcatccgc gagctcggcg
      781 tcgtttgcgc cctcctcgtc gtctgcgtct tgcgtctggt ccgccgacgg ctcacccgac
      841 tcatcgcgcg ccttcgtgtc cgccaccgcc tgtcccaagc gttccatgaa attccccatc
      901 gccccgcgtt cgcccgacgc tgctgctcct cctccgccgg cgtcatctcc cacgcgatgt
      961 cgggcgaaac cgtcatctga taactcaccg actcgatcac gacgcggccg ttcgtgaggc
     1021 tgaaccactc gaggtagagg ctgtttcccc agtgccaggt ccactttttc ttcgccgcat
     1081 aatactcgcc gatctgatcc atcgggatgt ccggcacctt cactttccgc gacgccgtgc
     1141 aatcgccgat cacgcccgtc tgccgcgcgg caaaccgctc cagcatttcg agcgccttcg
     1201 gctgcgggtt cacgaactcc agccgccggc ccgccagatc gcgccacgca tcgcccgcga
     1261 gatcgagttc caccggtgcc cccttgccga cgaaccagat ccgacccgtc acgcgcccac
     1321 gcacgcgatt gtcgatttcg ccgcgaatca ccgcctcgtc gatacgccac gccatgcccg
     1381 cgacgctgca aaaaccgccg gcccgcgcaa tcttccagaa tggtccggcc cggcgcccaa
     1441 caccccggca cccgccaccc ctttcgcggt cgatacagca tgcaagttca acgtgcccgc
     1501 caccgggctg cggccctcgc cgcctgcgcc ttcgccctcc tcgcgaccgg ctgctcctcc
     1561 acctactaca acgcgatgga gaaaatcggt ttcgccaaac gcgagatcct cgtcgaccgc
     1621 gtcgaagaca cccgcgacgc gcaaaacgac gccaaggaac agttcgccag cgcgctcgag
     1681 cagtt
//
LOCUS       DASPLT010001520         1652 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_18599, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001520 DASPLT010000000
VERSION     DASPLT010001520.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1652)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1652)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1652
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_18599"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            395..766
                     /locus_tag="VEA63_15520"
     CDS             395..766
                     /locus_tag="VEA63_15520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HYD85475.1"
                     /translation="MTTKEIAERLVSLCRQGQFETVHKELFATDAISIEPYATPTFPK
                     ETSGLNALLEKGEKFTRLIEQVHAISISDPLVAGSSFACAMQLDVTIKGHGRMNLDEL
                     CIYEVKDGKIVAEQFTSRFPV"
     gene            889..1311
                     /locus_tag="VEA63_15525"
     CDS             889..1311
                     /locus_tag="VEA63_15525"
                     /inference="COORDINATES: protein motif:HMM:NF016701.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HYD85476.1"
                     /translation="MITTPFTGGCQCGAVRYECDARLDEIFMFKCHCRDCQRLSGGAF
                     TPVVFVPRERLRITRGEIRRHATQGESGEHIRGFCPSCGSRLTGGEGPGSPGIGVTAA
                     SLDDPSGFEPQMEIWVSDAQPWDILNPALPHLPQNPPP"
BASE COUNT          318 a          495 c          511 g          328 t
ORIGIN      
        1 ggcgagacga gaggtgagac gctggcacat ggatcggttc atcggagttt gatgcgttag
       61 tagcgccgag cggatctttt cttacacctg gcgaaaaatt cgcaggcagg tgcgcggggg
      121 catgggtgac ggtcgacggc gatgcgaagc agccgatacg tgagatcttg cctctcgaag
      181 cggttcctcg tgcgtgcgtc ggattagcgc tcgcgcccaa ctgcgtcgtt tttcggcaac
      241 ggcttatcgg cgttacgcgc ctgggacgat ccgcgcagcc gtgcttcgcg gccaacgcac
      301 cgcgccgaaa atttcgcaac cgcatcgccg aactacgtgt cacgtttcgt gcgacccgcc
      361 cttggaaata gacgatcccc tcaactgaag aatcatgaca accaaagaaa ttgccgagcg
      421 actggtatcc ctgtgccgac agggccagtt cgaaaccgtg cacaaggagc tgttcgccac
      481 ggacgcgatc agcatcgaac cctacgccac gccgaccttc cccaaagaaa ccagcggcct
      541 taacgcgctc ctcgagaagg gagaaaaatt tacccgcctg atcgaacagg tgcacgcgat
      601 ctccatttcg gatccgctcg tggcaggcag ttcgttcgcc tgtgcgatgc agctcgacgt
      661 cacgataaaa ggacacgggc ggatgaacct ggacgagctt tgcatctatg aagtcaaaga
      721 cggcaagatc gtcgccgagc agtttacgtc gcggtttccg gtctaggcgg tgcagcaaga
      781 gagcgggtat gaaagggcga cggcttgatt ctcacctgtc cgaaaacgcg cggctcgttc
      841 gtcgatcttc aaaacgcgtt tctcagccaa gtttcagctt ccctcaccat gatcacgact
      901 ccttttaccg gcggctgtca gtgtggcgcc gtgcgttacg agtgcgacgc ccggctcgac
      961 gagattttta tgttcaagtg tcactgccgc gattgccagc gtctgtccgg cggggcgttt
     1021 actcctgtcg tcttcgtgcc gagggagcgg ctgcgtatca cgcgaggcga gatccggcgc
     1081 catgcgaccc aaggcgagtc gggcgaacat atccgcggct tctgcccgtc gtgcggctcc
     1141 cgcctcaccg gcggcgaggg gcccggctct cctggcatcg gcgtcacggc ggcgagcctg
     1201 gacgatccga gcggattcga accgcagatg gaaatctggg tcagcgatgc gcagccgtgg
     1261 gatatattga atccagccct accgcatctc ccgcagaacc cgccaccgtg aaacgtgtcc
     1321 accagcgaat ccgcgcgagt cgcagtggct ggcgcgccgg atccctatat gacgtctcca
     1381 acgcggcaga agatgatctt gccgggcggg actgaacttt cgttttttga cggctggaaa
     1441 cgcatcgaac gccgcggtgc tcctgttgca tgggtttcca aattcggcgc gcggcttccg
     1501 tgatgtgatc ccgatattgg cgcgagaggc gtatgtgatc gcgcccgacc tgccgggctt
     1561 cggcgagtcc gcgccgttgc ccaaggcttc gtttgccgcg ttgtcgctga tgagacgcgc
     1621 gtcagaatct gcgcgtaagg aatggcggcg ag
//
LOCUS       DASPLT010001521         1513 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_242506, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001521 DASPLT010000000
VERSION     DASPLT010001521.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1513)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1513)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1513
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_242506"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..465)
                     /gene="pgi"
                     /locus_tag="VEA63_15530"
     CDS             complement(<1..465)
                     /gene="pgi"
                     /locus_tag="VEA63_15530"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375827.1"
                     /note="functions in sugar metabolism in glycolysis and the
                     Embden-Meyerhof pathways (EMP) and in gluconeogenesis;
                     catalyzes reversible isomerization of glucose-6-phosphate
                     to fructose-6-phosphate; member of PGI family; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HYD85477.1"
                     /translation="MTWTRFKTHFYHNADLGVSLDISRIPFPDDFLTSMEPKIQEAFT
                     AMVALEGGAIANPDENRMVGHYWLRAPHLAPTPELTNEITTTLAAIKEFTAKIHSGEI
                     SGPKGKFKNLLVIGIGGSALGPRLALEALRGEADGPEVRFVANIDAAEFDDAI"
     gene            complement(563..>1513)
                     /locus_tag="VEA63_15535"
     CDS             complement(563..>1513)
                     /locus_tag="VEA63_15535"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009513291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HYD85478.1"
                     /translation="LKPRDILTRQAFENAIVICTALGGSTNLILHLLAIAHAAEVPLT
                     IEDFKIIGERVPLLADLKPFGKYNMSHLIRIGGIRPMMKLLLDRGLLHGHCLTVTGET
                     IAETLKDVQPYGAYPNEQDIIRPWDNPIKKETHLRVLHGNLAPGGAVGKITGKEGLYF
                     KGTAKVYESEEDALKGILRGDVVKGDVVVIRNEGPVGGPGMREMLSPTSAVAGRGLIK
                     DVALITDGRFSGGSHGFDVGHITPEAACGGPIGIVRSGDLIEVDAVKNTISLLIPDAD
                     YASRMAANKPRPPTETRGVLAKYAKLVSTASEGAVTDKYL"
BASE COUNT          237 a          390 c          570 g          316 t
ORIGIN      
        1 gatcgcgtcg tcgaactcgg cggcgtcgat gttggcgacg aagcggacct cggggccgtc
       61 ggcctcgccg cgcagcgcct cgagcgcgag ccgcgggccg agcgcggagc cgccgatgcc
      121 gatgacgagg aggtttttga atttgccctt ggggccggag atttcgccgg agtggatttt
      181 cgcggtgaac tctttgatcg cggcgagcgt ggtcgtgatt tcgttcgtga gctcgggcgt
      241 gggcgcgagg tgcggcgcgc ggagccagta gtggccgacc atgcggtttt cgtcaggatt
      301 cgcgatcgcg cctccttcga gcgcgaccat ggcggtgaac gcttcctgga tcttcggctc
      361 catcgacgtg aggaagtcgt ccgggaatgg gatgcgactg atatcgagcg agacgccgag
      421 atcggcgttg tggtagaagt gggttttgaa gcgagtccag gtcatgggcg aaggtttggt
      481 tgcccgcgaa tcacgagaat ggtcgcgaat aataaaatcg aattcgcgag agttggtgtg
      541 attcgcggga ggtgattttc gttcagagat acttgtcggt gacggcgcct tcggaggcgg
      601 tgctgacgag cttggcgtat ttggcgagga cgccgcgggt ctcggtcggc gggcgcggtt
      661 tattcgcggc catgcgggac gcgtagtcgg cgtccgggat gagcagcgaa atggtgtttt
      721 tcacggcgtc gacttcgatg aggtcgccgc tgcgcacgat gccgatcggg ccgccgcacg
      781 cggcttcggg cgtgatgtgg ccgacgtcga aaccgtggct gccgccggag aaacgaccgt
      841 ccgtgatgag tgcgacgtcc ttgatgaggc cgcggccggc gacggcgctg gtgggcgaga
      901 gcatttcgcg catgccgggg ccgccgacgg ggccctcgtt gcggatgacg accacgtcgc
      961 ccttcacgac gtcgccgcgg aggatgcctt tgagcgcgtc ttcctcgctc tcgtaaacct
     1021 tcgcggtgcc tttgaagtag agaccctcct tgccggtgat tttaccgacg gcgccgcccg
     1081 gggcgagatt gccgtggagc acgcggagat gggtttcctt tttgatcggg ttatcccacg
     1141 gacgaatgat gtcctgttcg tttggatacg cgccgtaggg ttgaacgtcc ttgagcgttt
     1201 ccgcgatggt ttcgccggtg acagtgaggc agtggccgtg gagcagaccg cgatcgagga
     1261 gcagcttcat catcgggcgg atgccgccga tgcggatgag atggctcatg ttgtatttgc
     1321 cgaacggctt caggtcggcg aggagcggaa cgcgttcgcc gatgattttg aaatcctcga
     1381 tggtgagcgg gacttcggcg gcgtgggcga tggcgagcag atggagaatg agattggtgg
     1441 agccgccgag ggcggtgcag atgacgatcg cgttttcgaa cgcctggcgc gtgaggatgt
     1501 cgcggggctt gag
//
LOCUS       DASPLT010001522         1524 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_57754, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001522 DASPLT010000000
VERSION     DASPLT010001522.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1524)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1524)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1524
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_57754"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1162)
                     /locus_tag="VEA63_15540"
     CDS             complement(<1..1162)
                     /locus_tag="VEA63_15540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HYD85479.1"
                     /translation="MLTRVAAPALTEPQLFAALHSELSAWFRARFTHFSPAQLHAVPE
                     ILADRSLLLTSPTGSGKTFAAFLSVFDHLAKLHDADHLPNGIFAVYVSPLRALAYDLR
                     KNLLEPIEQLGWTWLRIAARTGDTTQKERAAQRRKPPHILVTTPESLTLLLSQPAWLP
                     ALRSVRFLIADELHALAENKRGSMLMVAAERLEEVVATHPTPNFELRTPNSARLARLI
                     RIGLSATVAPLDTMAEFLVGPGRPCRIAEITQRKPAKIEVFSPLREHAYPPAGYTATR
                     VLKELGALLVKHETTLIFTNTRSGAESIGLRLKQLLPELTDLIEVHHASLDRGVRLEI
                     EDRLKRGELRAVVCSTSLEMGIDIGSIDLVVMVSAPKGVARALQRLGRSGHSM"
BASE COUNT          313 a          345 c          582 g          284 t
ORIGIN      
        1 ccatggagtg acctgagcgg ccaaggcgct ggagcgcgcg agcgacgcct ttcggtgcgg
       61 agaccatgac gacgagatcg atcgagccga tatcgatgcc catctccagg gaagtggagc
      121 aaacgacggc gcgaagctcg ccgcgtttga gccggtcttc gatctcgagg cgcacgccgc
      181 ggtcgagtga cgcgtggtgc acttcgatca agtcggtgag ttccgggaga agctgcttga
      241 ggcggagacc aatcgattcg gcgccgctac gggtgttggt gaagataagc gtggtctcgt
      301 gtttcacgag caacgcaccg agctccttca gcacacgcgt ggcggtgtag ccggcgggcg
      361 gatacgcgtg ctcgcggagc ggggagaaga cctcgatctt cgctggtttg cgctgggtaa
      421 tttcggcgat gcggcatgga cggccggggc caacgaggaa ctcggccatg gtgtcgagcg
      481 gagcgacagt ggcggagagg ccgatgcgga tcaggcgcgc gagacgcgcg gagtttggag
      541 ttcggagttc gaagttcgga gttggatggg tcgcgacgac ttcctccagc ctttcggcgg
      601 caaccatgag catcgagccg cgcttgtttt cggcgagggc gtggagttcg tcggcgatga
      661 ggaagcggac ggagcgcaga gcggggagcc atgccggctg gctgaggagg agggtgagac
      721 tctcgggcgt ggtgacgaga atgtggggcg gcttgcggcg ctgagcggcg cgttcctttt
      781 gcgtggtgtc gcccgtgcgg gcagcgatgc gcaaccacgt ccagccgagt tgttcgatgg
      841 gttcgagaag gttcttgcgc agatcgtaag cgagcgcacg caggggtgaa acgtagaccg
      901 cgaaaatgcc gttagggaga tggtcggcgt cgtggagttt ggcgaggtga tcgaaaacgg
      961 agaggaacgc agcgaaggtt ttgccgctgc cggtcggcga ggtgaggagg agcgaacgat
     1021 cggcgaggat ctcgggaacg gcgtggagtt gtgcgggaga aaaatgcgtg aagcgggcgc
     1081 gaaaccaggc ggagagttcg gaatggagcg cggcgaaaag ttgcggctcg gtgagcgcgg
     1141 gtgcggctac gcgggtgagc atggacgaat taaccacgga tgaacacggt gattgaaaaa
     1201 ggatcggagt tttgaaccac gaatggacac gaattcacac gaatgacgga ttgggattcg
     1261 cgagcctcgt gttgattcgt gtgtaaatgg gttggaatta accacggatg gacacggatt
     1321 cggaatgcga cctccggcgg gcggggtttg atctgtgttc atcagtgttc atccgtggtt
     1381 gagaaaaggg atcggagttt tgaaccacga agggacacga attcacacga atgacggatt
     1441 gggattcgtg agcatcgtgt tgattcgtgt gtaaatgggt tggaattaac cacggatgga
     1501 cacggattcg gaatgcgacc tccg
//
LOCUS       DASPLT010001523         1289 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_242536, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001523 DASPLT010000000
VERSION     DASPLT010001523.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1289)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1289)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1289
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_242536"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1040
                     /locus_tag="VEA63_15545"
     CDS             <1..1040
                     /locus_tag="VEA63_15545"
                     /inference="COORDINATES: protein motif:HMM:NF042648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="N,N-dimethylformamidase beta subunit family
                     domain-containing protein"
                     /protein_id="HYD85480.1"
                     /translation="EPLHEFELPKDWLSGVYLAKLTRAVDGFQAYIVFVVKDARRADF
                     NFQVSDMTWQAYNRWPAWRSLYDFQDELWIGSGGNAISFDRPYGFFFNLLPSKLNKYS
                     NGSGEFLMWEFPLAFWMEQHGYDVTYTSNIDTHADGAGLLRTKAFLSVGHDEYWTRQM
                     FDHVAAARDAGVNLLFLSGNAISGEMFLTTSISGQPNRVTGRTRKFTDEHELMGSTSY
                     GVGLGDWIVTQPDHWLYEGTGLKTGDAIPNLIGWEFHGPPLRDDPTLVVLGEARFRGD
                     REKPKRTHAATIYDGPKGNFVFNAGTCWWSMPLSTPPAFENPPDVDFTQNDPRVQRMT
                     KNLFERVLAER"
     gene            1084..>1289
                     /locus_tag="VEA63_15550"
     CDS             1084..>1289
                     /locus_tag="VEA63_15550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD85481.1"
                     /translation="MKTTHAILLSAIWAGAVFGAEELPEDVAPGQTGENKKEAPPELI
                     DPGSEKPKKIIGHKAHYDESGRLLA"
BASE COUNT          262 a          414 c          373 g          240 t
ORIGIN      
        1 gggagccact gcatgaattc gaactcccga aggactggct cagcggcgtc tacctcgcga
       61 agctcacgcg cgccgtcgat ggttttcagg cctacattgt tttcgtcgta aaggacgcgc
      121 gccgcgccga cttcaacttt caagtctccg acatgacgtg gcaggcctac aaccgctggc
      181 cggcgtggcg ctcgctctac gatttccagg acgagctctg gatcggttcc ggcggcaacg
      241 ccatcagctt cgaccggccc tacggtttct ttttcaatct gctaccgtcc aagctgaaca
      301 aatactccaa cggcagcggc gagtttctca tgtgggagtt tccgctcgcg ttctggatgg
      361 agcagcacgg ctacgatgtc acttacacgt cgaacatcga cacgcacgcc gatggcgccg
      421 ggctgttgcg cacgaaggcg tttctctccg tcggtcacga cgaatactgg acgcgccaga
      481 tgttcgatca cgtcgccgcc gcccgcgacg cgggcgtcaa cctgctcttc ctttcaggca
      541 acgcgatcag cggcgagatg tttctcacca ccagcatcag cggtcagccg aaccgtgtga
      601 ccggccgcac ccgcaaattc accgacgagc atgagctaat gggttcaacg tcttatggcg
      661 ttggcctcgg cgactggatc gtcacgcagc cggatcactg gctctacgaa ggcaccggcc
      721 tgaaaaccgg agacgcgatt ccgaacctga tcggttggga gtttcacggt ccgcctttgc
      781 gcgacgaccc gacgctcgtc gttctcgggg aggcgcgctt ccgcggcgat cgcgagaaac
      841 cgaagcgcac gcacgccgcc acgatctacg acggaccgaa aggcaacttc gtgttcaacg
      901 ccggcacctg ctggtggagc atgccgctgt cgaccccgcc cgcgttcgag aacccgcccg
      961 acgtcgactt cactcaaaac gatccacgcg tgcagcggat gacgaaaaat cttttcgaac
     1021 gcgtcctcgc ggaacgttga tcgagcggca gcgccgcaga tccgtcagtt tcaccttttc
     1081 aatatgaaga ctacacatgc gatcttgttg tcggcgatct gggccggcgc ggtttttggc
     1141 gcggaagaac tgcccgaaga cgtcgcgccc ggccagaccg gcgaaaacaa aaaggaagcg
     1201 ccgcctgagc tgatcgatcc cggcagcgaa aagccgaaga agatcatcgg gcacaaggct
     1261 cactacgatg aatcagggcg gctgctcgc
//
LOCUS       DASPLT010001524         1433 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_57803, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001524 DASPLT010000000
VERSION     DASPLT010001524.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1433)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1433)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1433
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_57803"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..321
                     /locus_tag="VEA63_15555"
     CDS             <1..321
                     /locus_tag="VEA63_15555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD85482.1"
                     /translation="EILCDRIVILNRGAVVAAGAPRELIERETPGFVGIFAHDAEPRL
                     RPHVAEKWILFHQGRQCCVRTPEIDDLVALQHAAGLSALQLRPANLEDVYLKLTGQEL
                     DDDE"
     gene            314..1090
                     /locus_tag="VEA63_15560"
     CDS             314..1090
                     /locus_tag="VEA63_15560"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HYD85483.1"
                     /translation="MSEWTISLRLGWHVVVRNWTVYRKDFIANISPTLADPALLLTSL
                     GMGLSPFIGGINGLTYAQYLAPGMVATTALFSAFFDSSYGFYVRMTFESIFKAMLTTP
                     IGVREVVIGEFLWSAIRAAMMGGSVGLVLAVMGLLPNPWALLAFPFIAGTLAVPCAAI
                     GLLAAGFVRNMNQFQTVYSFLIAPIFYLSAVFFPLDDRPILGLVINLSPFYHGVRLAQ
                     MTAWGRWNFPELALHLGALILFTLVLGLWASRRIRRKLIS"
BASE COUNT          229 a          535 c          356 g          313 t
ORIGIN      
        1 gaaattctct gcgaccgcat cgtcatcctc aatcggggcg ccgtcgtcgc cgctggcgcc
       61 ccgcgcgaat tgatcgaacg cgaaacgccc ggtttcgtcg gcatcttcgc gcacgatgcc
      121 gaaccgcgcc tccgccctca cgtcgccgaa aaatggatac tcttccacca gggtcgccag
      181 tgttgcgtcc gcacgccgga gatcgacgac cttgtcgcgc tccagcacgc cgccggactc
      241 agcgcgctgc agctccggcc cgctaatctc gaggacgttt acctgaaact caccggacag
      301 gagctcgacg acgatgagtg agtggacgat ctccctacgc ctcggctggc acgtcgtcgt
      361 tcgcaactgg acggtttatc gaaaagattt catcgcgaac atctcgccga cactcgccga
      421 tcccgcactg ctgctcacct cgctcggcat gggcctctcg ccgttcatcg gcggcatcaa
      481 cggcctcact tacgcgcaat acctcgcacc cggcatggtc gccacgaccg cgctcttctc
      541 ggcattcttc gacagcagct acggcttcta cgtgcgcatg acgttcgagt cgattttcaa
      601 ggcgatgctc accacgccga tcggcgtgcg cgaagtcgtg atcggcgaat ttctctggag
      661 cgccatccgc gccgcgatga tgggcggctc cgtcggtctc gtgctcgctg ttatgggcct
      721 cctgcccaat ccctgggcgc tgctcgcgtt tcccttcatc gccggcacgc tcgccgtccc
      781 gtgcgccgcc atcggcctgc tcgccgccgg tttcgtgcgc aacatgaacc agtttcagac
      841 cgtttactcg ttcctcatcg cgccgatctt ttatctgtcc gccgttttct tcccgctcga
      901 tgaccgcccg atcctcggcc tcgtcatcaa cctctcgccg ttttaccacg gcgtccgcct
      961 cgcccaaatg accgcttggg gccgttggaa cttcccagaa ctcgcgctcc acctcggcgc
     1021 cctcatcctc ttcacgctcg tcctcggcct ctgggcctcc cgccgcatcc gccgcaagct
     1081 catcagctaa actgtgggag gggctttcgc cccgactcct cgtccgccgt ccaccacgcc
     1141 cgcgcggctc ggaacaattc atttgaacca cgaatcgaca cgaagaaaac gcgctcgaat
     1201 gcggagcctt atgggaacaa cgaaggggtg acgtaacgat cagcctcaaa gctttccttc
     1261 ggctcctttt gaattcgtgt ccattcgtgg ttccccttct catcagtccc accaaggcgc
     1321 ctcccacctc caatcctccc gccaaattct ttcgtgtctt tcccgcgttt cgtggttagc
     1381 tccgccttcc acgtcaccgc catgtccgat ctcgtccaac tcgtcacctc gat
//
LOCUS       DASPLT010001525         1147 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_141946, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001525 DASPLT010000000
VERSION     DASPLT010001525.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1147)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1147)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1147
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_141946"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1147
                     /locus_tag="VEA63_15565"
     CDS             <1..>1147
                     /locus_tag="VEA63_15565"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HYD85484.1"
                     /translation="QLNQVIGAGGAREHGVLSWETVLASASSRFTPCDTTADDPAFII
                     YTSGTTGPAKGALLAHRCVIGNLPGFVHSHDFYPKQGDMFWSPADWAWTGGLMDALLP
                     AWAFGQPLLGYRGRFEAEKAFYLLEKYAVRNAFIFPTALKFMMKAVPEPAKRYDLGLR
                     TLMSAGESVGVPVIEWATEQLGVTINEMFGQTEANYIVGNCQAAWPVKPGSIGRPYPG
                     HRVAIVDAEGNEVRRGELGEIAVGRSGDPVMFLEYWRNAQATAEKFVGDWALTGDQGR
                     MDEDGYIWYQGRTDDVIKCAGYRIGPAEIESCLVRHPAVANAAVVGKPDDARGMIVKA
                     FVVLQPDAVGSEALVEDIQQHVRGRLAPYQYPREIEFVAALPMTPTG"
BASE COUNT          213 a          301 c          427 g          206 t
ORIGIN      
        1 cgcaattgaa ccaggtgatc ggcgcgggtg gcgcgcggga acacggcgtg ctgtcgtggg
       61 aaaccgtgct cgcgtcggcc agcagccggt tcaccccgtg cgacacgacc gccgacgatc
      121 cggcgttcat catctacacg agcggcacga cgggtccggc aaagggagcg ctgctggcgc
      181 atcgctgcgt gatcgggaat ctgccggggt tcgtgcactc gcatgatttt tatccgaagc
      241 agggcgacat gttctggtcg ccggcggact gggcgtggac gggcgggctg atggacgcgt
      301 tgttgccggc atgggcgttc gggcagccgt tgctcggcta tcgcggtcgg ttcgaggcgg
      361 agaaggcgtt ttatcttttg gaaaaatacg cggtgcgaaa cgcgttcatt tttccgacgg
      421 cattgaaatt catgatgaag gcggtgccgg agccagcgaa gcgctacgat ctcgggctgc
      481 gcacgctgat gagcgcgggc gaatcggtcg gtgtgccggt gatcgagtgg gcgacggagc
      541 aactcggcgt gacaatcaac gaaatgttcg ggcaaacgga ggcgaattac attgtcggca
      601 actgccaggc ggcgtggccg gtgaaacccg gttcgatcgg acggccgtat ccggggcatc
      661 gcgtcgcgat cgtcgacgcc gaaggcaacg aagttcgccg cggtgaactt ggggagatcg
      721 cagtcgggcg aagcggcgat ccggtgatgt ttctcgaata ctggcgcaac gcgcaggcga
      781 cggcagagaa gtttgtcggc gactgggcgc ttacgggcga tcagggtcgg atggatgaag
      841 acggctatat ttggtatcag gggcgaacgg atgacgtgat caaatgcgcc ggttaccgga
      901 tcggaccggc agaaatcgaa agttgtctcg tgcggcatcc ggcggtggcg aacgcggcgg
      961 tcgtgggcaa gcccgacgat gcgcggggca tgatcgtgaa ggcgttcgtt gtgttgcagc
     1021 cggacgcggt gggatcggaa gcgctcgtgg aagacatcca gcagcatgtg cgcggccggc
     1081 tcgccccgta tcagtacccg cgggaaatcg aatttgtggc ggcgctgccg atgacgccca
     1141 ccggcaa
//
LOCUS       DASPLT010001526         1143 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_57820, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001526 DASPLT010000000
VERSION     DASPLT010001526.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1143)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1143)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1143
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_57820"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..624
                     /locus_tag="VEA63_15570"
     CDS             <1..624
                     /locus_tag="VEA63_15570"
                     /inference="COORDINATES: protein motif:HMM:NF039702.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6250 domain-containing protein"
                     /protein_id="HYD85485.1"
                     /translation="TTELVRERFEDDRWRKRWKLEGNATLAVNDGRLSIVTSDAPGVE
                     PAATLWWAEALPADVLIEAIAGVDEPAENNAANLNLILHASEPDGPYRFGRSGRYGEY
                     QKIPNYIVTLTGGFQEGWSRVRRNPGFVQLSEERSTRSEIGRTYRIRVLLAGGRLRYW
                     LDGRLVHDVRDSQPLDGGHFALRTWRSRVWWSDISIASVRRLGSEQP"
     gene            671..>1143
                     /locus_tag="VEA63_15575"
     CDS             671..>1143
                     /locus_tag="VEA63_15575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HYD85486.1"
                     /translation="MKPTVTLSDITAAARRIEGDVLRSPCPESISLGEITGARVSCKL
                     DNLQRTGSFKERGARNALTRLSPAQRRVGVIAASAGNHALGLAYHGKLLGIPVTVVMP
                     NHAPLIKVATCERMGARVVMGGQDFSEVRALADTLAIEQGLSYINGYDDPAIIAG"
BASE COUNT          191 a          332 c          399 g          221 t
ORIGIN      
        1 acgaccgagc tggtccgtga gcggttcgag gatgatcgct ggcgcaagcg ttggaagctg
       61 gagggcaacg cgacgcttgc cgtgaatgac ggacggctgt cgatcgtcac gagcgacgcg
      121 ccgggcgtcg aaccggcggc gacactttgg tgggcggagg ccttgccggc ggacgtgttg
      181 atcgaggcga tcgcgggcgt cgacgaaccg gcggagaata acgcggcgaa tctgaatctg
      241 attctgcatg cgagcgaacc ggacgggccg tatcggtttg gccgttcggg gcgctacggc
      301 gaatatcaga aaattccgaa ttacatcgtg acgcttaccg gcggatttca agaaggctgg
      361 tcgcgcgtgc ggcgaaatcc tggcttcgtt cagttgtcgg aagagcgctc gacgcgctcc
      421 gagattggcc ggacttatcg gattcgcgtg ctcctcgccg gcgggcggct gcgttactgg
      481 ctcgacggtc ggctcgtgca cgacgtgcgc gattcgcagc cgttggacgg tgggcacttc
      541 gcgctgcgga cgtggcgttc gcgcgtgtgg tggtccgaca tttctatcgc gagtgttcgc
      601 cggctcggct ccgaacaacc gtagctgcgg tttcgatacc gcgcgcggca cgcttcgatt
      661 tagctcaacc atgaaaccaa cggtcacgtt gtccgatatc accgcggccg cgcgccgcat
      721 cgagggcgac gtgttgcgtt cgccttgtcc cgaatcgatt tcgctgggcg agatcaccgg
      781 tgcgcgcgtg agctgcaaac tcgacaatct gcagcgcacc ggatccttca aagagcgcgg
      841 cgcgcgcaat gcgctcacgc gtctttctcc cgcgcaacgt cgtgtcgggg tgatcgcggc
      901 ttccgcggga aatcacgcgc tgggcctggc gtatcacggc aagttgctcg gcatcccggt
      961 gacggtcgtc atgccgaacc atgcgccgtt gatcaaggtg gccacgtgtg agcggatggg
     1021 tgcgcgcgtg gtgatgggcg gacaggattt ttcggaggtt cgggcgctcg ccgacacact
     1081 ggcgatcgag cagggcttgt cctacattaa cggatacgac gaccccgcaa tcatcgcggg
     1141 gca
//
LOCUS       DASPLT010001527         3587 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_18679, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001527 DASPLT010000000
VERSION     DASPLT010001527.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 3587)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3587)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3587
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_18679"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(144..665)
                     /locus_tag="VEA63_15580"
     CDS             complement(144..665)
                     /locus_tag="VEA63_15580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheD"
                     /protein_id="HYD85487.1"
                     /translation="MGSATIASIFAQRVVVGVGDMAVSNNALVTLSTYALGSCIGVVA
                     YDPAVKVGGILHMMLPDSKISPDKAAKQPAMFADTGLPLFFRSLAGLKAERARLRIFV
                     TGGAGILASHDNFKIGERNTKATLEYLAANGYRVRQQVTGGTTNRTVHLEVGAGGMIL
                     KTPEGSETISLAA"
     gene            complement(962..2242)
                     /locus_tag="VEA63_15585"
     CDS             complement(962..2242)
                     /locus_tag="VEA63_15585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HYD85488.1"
                     /translation="MTSSTPAASFDPSTILIVDDEPVVLTALQQTLERERFHVVACSS
                     PTKALAILAERDFAVIISDQKMPEMLGLDFLIESRRMRPHASRILITAVLSLPTIVDA
                     INKGEIFRFVAKPWLREELIATVRNAVHRHELVTHNAVLQAQTQSLNEQLTQANTALA
                     QQVKDLEQQRQRLDTANRELAANYEHSLELCRRILTTYDPILGGQAKALVEFAGHMAE
                     SDLLDETERHALKTGAWLCDIGLIGVPREMLRAFRSKMEDLTDRERTMMHNHPVYSQT
                     LAALVDDNARVGEVIRAHHERYDGTGYPDGLAGDAIPWAARCLAVAVGYVESGLPKAS
                     AIDLILSQSGTAYDPEAVRLFLKVTNLAQLPRQVREITLHELEPGMVLANGIYSPHGL
                     LLIGEGQELSPATIAKIRSHNQVTPISQRLLVRI"
     gene            complement(2239..3336)
                     /locus_tag="VEA63_15590"
     CDS             complement(2239..3336)
                     /locus_tag="VEA63_15590"
                     /EC_number="3.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_function: GO:0050568 - protein-glutamine glutaminase
                     activity [Evidence IEA];
                     GO_process: GO:0006482 - protein demethylation [Evidence
                     IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0018277 - protein deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator protein-glutamate
                     methylesterase"
                     /protein_id="HYD85489.1"
                     /translation="MSRRIRVLIVDDSAVVRKLVTDALSADPEIEVVGTAIDPYVARD
                     KIKQLNPDVLTLDLEMPRMDGLTFLRILMEQHPLPVVIMSSLSQRGSDYALEALRLGA
                     CDVLGKPAGSYSFGDLGPQLIARIKAAAGARLSARRPTGSAASANAATNAARAQPRTT
                     TASARQVILLGASTGGTEALREVLTRLPAGLPPIAIVQHIPPVFSKAFADRLSTQCAF
                     QVREAVDGERLEPGVALVAPGNFHLMIQWHGNHYRARVTGGPQVWHQRPAVDLLFKSA
                     ADCGAAPHAIAGVLTGMGKDGAEGLLRLREHGAVTFAQDEASCVVYGMPRVAWENGGA
                     QRQISLDRIADFIVHPPLPTSDANLLASAQP"
     gene            complement(3333..>3587)
                     /locus_tag="VEA63_15595"
     CDS             complement(3333..>3587)
                     /locus_tag="VEA63_15595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CheR family methyltransferase"
                     /protein_id="HYD85490.1"
                     /translation="VTFRHLNLLEGEPPFREPFQAIFCRNVMIYFDRPTQEELVNKLA
                     RHLVPGGYLFVGHAESLTAIKHSLQTVRPAVYRRPANATP"
BASE COUNT          623 a         1081 c         1248 g          635 t
ORIGIN      
        1 gcgctgctga tgctgtcggc ggcgctggct tgttggctgc cggcccggcg cgcgggccgg
       61 gtggatatca cgaggttgtt gcgcgcggaa tagcgggcag cgaggcggtc aaacgtcggc
      121 gaaacgatag cgctttccgc cgtttacgcc gcgagcgaga tcgtttcgct gccttcgggc
      181 gttttcagga tcatgccgcc ggcgccgact tcgaggtgca ccgtgcggtt ggtcgtgccg
      241 cctgtcacct gttggcggac acgatagccg ttcgccgcga ggtattcgag tgtcgccttc
      301 gtgttgcgct ctccgatttt gaaattgtcg tggctcgcga gaatgcctgc gccgcccgtc
      361 acgaagatcc gcagtcgcgc acgctcggct ttcaagccgg cgagcgagcg gaaaaagagc
      421 ggcaggcccg tgtcggcgaa catcgcgggc tgcttcgcgg ccttgtccgg cgagatcttc
      481 gaatccggca gcatcatgtg caggatgccg ccgaccttca cggccggatc gtaggcgacc
      541 acgccgatgc acgagccgag ggcgtaggtg ctgagcgtga caagggcgtt gttcgacacg
      601 gccatgtcgc cgactccgac gacgacacgt tgggcgaaga tggaggcgat agtggcggaa
      661 cccatgcgtc aggcagcgga gacgacgaac gcggctccga acagtggtgg tgtggaaaaa
      721 ggcatgtaag cggagcggtt tggcgtggaa tccgcgtgga aaatatacgg ttgcggtccg
      781 acaaacttga gggcggttcg gaacaaaatg gcgggcgcgc gctgcggcgg cgcggcagat
      841 ggagcttccc tgcgctcacg cgcagagcca ccgatgccat ggcctcgcag gccatgtttc
      901 ttcgatgtgg ctcgctccgt aaggagcggg acggaaaatc cttcgcaagc tttgccgccg
      961 ctcaaatccg cacgagcagc cgctggctga tcggcgtcac ttggttgtga ctgcggatct
     1021 tcgcgatcgt cgccgggctg agctcctggc cttcgccgat gagcagcaag ccgtgcgggc
     1081 tgtaaatacc gttggccagc accatgcccg gctcgagttc gtgcagcgtg atttcgcgaa
     1141 cctgccgcgg cagttgcgcg agattcgtga ccttgaggaa aagtcgcacc gcctcaggat
     1201 cgtaggccgt tcccgactgc gaaagaatga ggtcgatcgc cgacgccttc ggcaaacccg
     1261 actcgacgta gccgaccgcc accgcgaggc agcgcgcggc ccacggaatc gcgtcacccg
     1321 ccagcccgtc gggataaccg gtgccgtcgt aacgttcgtg gtgcgcgcga atcacttcgc
     1381 cgacgcgcgc attgtcgtcg accagcgcgg cgagcgtctg gctgtagacc ggatgattgt
     1441 gcatcatcgt gcgctcgcga tcggtgaggt cttccatctt cgagcggaac gcgcgcaaca
     1501 tctcgcgcgg cacgccgatc aacccgatgt cgcacaacca cgctccggtt ttcaacgcgt
     1561 ggcgctccgt ctcatcgagc aaatcgctct cggccatgtg gccggcgaat tccacgagcg
     1621 cctttgcctg gccgccaagg atcggatcgt aggtcgtgag aatgcggcgg cacaactcga
     1681 gggagtgctc gtagttcgcg gcgagctcgc ggttcgcggt gtcgaggcgc tggcgctgtt
     1741 gctcgagatc cttcacctgc tgcgcgagcg cggtgttggc ctgcgtgagc tgctcgttca
     1801 agctttgcgt ctgggcctgc agcaccgcgt tgtgcgtgac gagctcgtgg cgatgcacgg
     1861 cgttgcgcac ggtggcgatg agctcttcgc gcaaccacgg tttcgcgacg aagcggaaaa
     1921 tttcgccctt gttgatcgca tcgacgatcg tcggcaacga cagcacggcc gtgatcagaa
     1981 tgcgcgatgc atgcgggcgc atgcgccggc tctcgatcag aaaatcaagg ccgagcatct
     2041 ccggcatttt ctggtcggag atgatgaccg caaaatcgcg ctccgccaga atcgcgagcg
     2101 ccttcgtcgg actcgagcac gcgaccacgt gaaaccgctc gcgctcgagc gtctgctgca
     2161 acgccgtgag caccaccggc tcatcgtcga cgatgagaat cgtggaggga tcgaagctgg
     2221 cggcaggggt cgaagaggtc atggctgggc cgatgcgagc aagttcgcgt cggaggtcgg
     2281 cagaggcgga tgaacgatga aatcggcgat gcggtcgagc gagatttgtc gctgcgcgcc
     2341 gccattttcc cacgcgaccc gcggcatgcc gtaaacgacg cagctcgctt catcctgggc
     2401 aaacgtgacg gcgccgtgct cgcgcaagcg cagcaatcct tccgcgccgt ccttgcccat
     2461 acccgtcaac acgccggcga tcgcgtgcgg cgccgcgccg caatcggcgg cggatttgaa
     2521 caagaggtcg accgcggggc gttggtgcca gacttgcggg ccgccggtga cgcgcgcgcg
     2581 atagtggttc ccgtgccact ggatcatgag atgaaaatta ccgggcgcca cgagcgcgac
     2641 gccgggctcg agccgttcgc cgtcgacggc ttcgcggacc tgaaatgcgc attgcgtgct
     2701 gagccggtcg gcaaacgcct tcgagaaaac gggcggaatg tgttggacga tcgcgattgg
     2761 cggaagtccc gcgggcaggc gcgtgagcac ctcgcgcagc gcctcggtgc cgcccgtgga
     2821 agcaccgagc aggatcactt gccgcgcgga cgccgtcgtc gtgcgaggtt gcgcgcgagc
     2881 ggcgttcgtg gcggcgtttg cggatgcggc agagccggtg gggcgtcgcg ccgacaggcg
     2941 tgcgccggcg gcggccttga tgcgcgcgat gagttgcggg ccaaggtcgc caaacgaata
     3001 cgagccggcg ggtttgccga gcacgtcgca cgcgccgagc cgcagcgctt cgagcgcgta
     3061 atcggagccg cgttgcgaga gcgaactcat gatcacgacc ggcagcggat gctgctccat
     3121 caaaatgcgg agaaacgtga gcccgtccat gcgcggcatt tccagatcga gcgtgagcac
     3181 gtcggggttg agctgcttga tcttgtcgcg tgcgacatac ggatcgatgg cggtgccaac
     3241 gacctcgatc tcgggatcgg cgctcaaggc gtcggtcacc agtttgcgga cgacggcgga
     3301 gtcgtccacg atcaggacgc gaatgcggcg gctcatggcg tggcgttggc gggccggcgg
     3361 taaacggcgg gccgcacggt ttggagcgag tgcttgatcg cggtgaggct ctcggcgtgt
     3421 ccaacaaaca aatacccgcc gggcacgagg tgccgcgcga gtttgttgac gagttcttcc
     3481 tgcgtcggcc gatcgaagta gatcatgacg ttgcgacaga agatcgcctg aaacggttcg
     3541 cgaaacggcg gttcgccttc gagcaggttg agatggcgaa acgtcac
//
LOCUS       DASPLT010001528         1329 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_99303, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001528 DASPLT010000000
VERSION     DASPLT010001528.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1329)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1329)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1329
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_99303"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(532..762)
                     /locus_tag="VEA63_15600"
     CDS             complement(532..762)
                     /locus_tag="VEA63_15600"
                     /inference="COORDINATES: protein motif:HMM:NF021085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD85491.1"
                     /translation="MSSARRATLRRTVAVEAGMTTKDYKAILSAVEQAIAICDSYGDR
                     IDDMKRKLCEDLRQARTELRRQLDQSPLTGSS"
     gene            952..>1329
                     /locus_tag="VEA63_15605"
     CDS             952..>1329
                     /locus_tag="VEA63_15605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD85492.1"
                     /translation="MNAAHFSRGLGWFSIALGLTELLAPKKLARAIGVGEGHENLLRA
                     LGAREIASGLGILARPKPTGMVWSRVAGDAMDLALLGFAYEARGTDRRRLTAAIIAVA
                     GITALDVATAIRLTQPPRADPRWR"
BASE COUNT          232 a          482 c          365 g          250 t
ORIGIN      
        1 cagcgcgccc tcggtcaacc cggtgcggtc accggcccgc tcaagctcga cacgaaatcg
       61 accggccgcc gcacgaagac ctctcgcgag tcctgtgtct tcacgcctta tcccggacgg
      121 ttcgcgctgc gattcctcgg cacgcccggc ggctaaacgc gcctcgcgga gagagcggcg
      181 gctgctcgcg ctcgcgcttt tattcctcgg aagaaagaga tccgcccgac gaccgcggcg
      241 acgatttgcg aacgcacgtc tcagcgagct cgtttcgcgc caccgagaga aggctccgaa
      301 tcctgacctc cttcgcactc aattgcccct cgtggtagcg aaacaattcc agcgcggaat
      361 ctatccagtc caggatgcgg cgggaatcat tttcagtcat gagcagcggt cactcggcgc
      421 ggacgcggcg aatgaagaaa tagagtaata gggagcgaag gatttgttcc gggagagaca
      481 agcctggttc gacccacgcg ccgtctttgt ccgctcttgc gtgtcctcaa ctcaggacga
      541 tccggtgagc ggtgactggt ccaactgacg ccgcaactcc gtccgcgcct gacgcaagtc
      601 ctcgcagagc tttcgcttca tatcgtcgat gcgatcccca tagctgtcac aaatcgcgat
      661 cgcctgctcc accgccgaaa ggatggcttt gtaatctttc gttgtcatgc cagcttcgac
      721 cgcgaccgtc cgccgcagag tcgcccgcct cgcggacgac aagccgattc gctcctaggt
      781 ttttccccga cgacctgctt cacctcccag tccgctcgca gcagcggaac ccgtccgccg
      841 cgccttcgat tccctcgggc ctcctgcgcc tcgcgccggc gtagattcca acacgctccc
      901 tcgccgtctt tcccccattt tgaaacccca cacacgctac gatagtagtg gatgaacgca
      961 gctcattttt cccgcggact cgggtggttt agcatcgcac ttggcctgac cgaactcctc
     1021 gcgcctaaaa aactcgcccg cgccatcggc gtcggcgaag gacacgaaaa tctcctccgc
     1081 gcgctcggag cgcgcgaaat cgccagtggc ctcggcatcc tcgcacgtcc caaacccacc
     1141 ggcatggttt ggtctcgcgt cgccggtgac gcgatggatc tcgcgctcct cggcttcgcg
     1201 tatgaggccc gcgggaccga tcgacgtcgt ctcaccgccg ccatcatcgc cgtcgcgggc
     1261 atcaccgcgc tcgatgtcgc gactgcaatc cggctgaccc agccgccccg cgccgatccg
     1321 cgctggcgc
//
LOCUS       DASPLT010001529         1050 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_283935, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001529 DASPLT010000000
VERSION     DASPLT010001529.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1050)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1050)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1050
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_283935"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..421
                     /locus_tag="VEA63_15610"
     CDS             <1..421
                     /locus_tag="VEA63_15610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD85493.1"
                     /translation="TPDFTSRLLNRVSNATESPWEGQMGMLHEFESTDWDGLRTSLGY
                     AFEGVMIDIPNAVTLKLVGEWRAGDGGDMLERRMGVGMADMLFKAHEGYLSFSQAKPH
                     DYNWEKTLVPMGADLIVPLWETFFAPPPPPSKPAASR"
     gene            complement(545..>1050)
                     /locus_tag="VEA63_15615"
     CDS             complement(545..>1050)
                     /locus_tag="VEA63_15615"
                     /inference="COORDINATES: protein motif:HMM:NF013847.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD85494.1"
                     /translation="GLPAACVQVDEANIPGNPADAPLAAASMNLVLDAIDRGTERAVH
                     FCFGNYGGQTIQKGNWQKLLGFLNALHTDHLVLELAHRPASDLDALKRIDKKISLGVG
                     VIDIKVNHIETPDEVAARIEAVEKKVGADRVRWVHPDCGFWMLKRSIADRKIEALVRG
                     RDKYLGR"
BASE COUNT          185 a          362 c          304 g          199 t
ORIGIN      
        1 gacgcccgac ttcacctcgc gcctgctcaa ccgcgtttcc aatgccaccg aatcgccgtg
       61 ggagggccag atggggatgc tgcacgagtt tgagagcacc gattgggacg gcctccgcac
      121 aagtctcggc tatgctttcg agggcgtcat gatcgacatt ccgaatgccg tgacgctcaa
      181 gctcgtcggc gagtggcgcg ccggcgatgg cggcgacatg ctcgagcgcc gcatgggcgt
      241 cggcatggca gacatgctgt tcaaagctca cgaaggttat ctcagttttt cgcaagccaa
      301 gccgcacgac tacaactggg agaaaaccct cgtccccatg ggggccgacc tgatcgtccc
      361 gctctgggaa actttcttcg ccccgccccc gccgccgtcc aagcccgcgg ccagccgctg
      421 acgctcaggt agggacgcct ctccgaggcg tccgctggcg catcgtttct ctcctgcccc
      481 tcgcgccagc ggacggctcg gagagccgtc cctaccacga ttaggtcgcg acaaccgatc
      541 gacgtcagcg cccaagatac ttgtctcgtc cgcgcaccag cgcctcgatc tttcgatccg
      601 caatcgagcg cttcagcatc caaaatccgc agtccgggtg aacccagcgc acgcggtccg
      661 cgccgacctt tttctccacc gcttcgatcc gcgcggcgac ttcatccggc gtttcaatgt
      721 ggttcacctt gatgtcgatc acgcccacgc ccaacgagat tttcttgtcg atgcgcttga
      781 gcgcgtcgag atccgacgcc ggtcgatgcg cgagttcgag gacgaggtga tccgtgtgga
      841 gcgcgttgag aaagcccagc agcttttgcc agttaccctt ctggatcgtc tggccgccgt
      901 agttgccaaa acagaaatgc accgcgcgct ccgtgccgcg atcgatcgca tcgagcacga
      961 gattcatcga tgcggccgcg agcggagcgt ccgcgggatt gcccggaata ttcgcctcgt
     1021 ccacttgcac gcacgccgcc ggcaagcctc
//
LOCUS       DASPLT010001530         1107 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_141967, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001530 DASPLT010000000
VERSION     DASPLT010001530.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1107)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1107)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1107
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_141967"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..822)
                     /locus_tag="VEA63_15620"
     CDS             complement(<1..822)
                     /locus_tag="VEA63_15620"
                     /inference="COORDINATES: protein motif:HMM:TIGR03871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HYD85495.1"
                     /translation="MPCLRILTVCLWIFVGVRMVAAEPERVLRVAADPNNLPFSNERC
                     EGFENRIAELVARELGARLEYFWWPQRRGFFRQTIGAGRADLVIGVAAGIDHVLTTRP
                     YYRSTYVFVQRAGTGREVTSFDDPRLHELTIGVQMVGDDFNNTPPAHALSRRGMIENV
                     RGYSLYGDYAQESPPAEIMRAVARGEIDVAVAWGPMAGYFARRQAVPLDVTPVTPERD
                     GPVLPLTYAIAMGVGRGDAALKAEVDAVIERRGAEIAKILAEYGVPCLELKGSGAE"
     gene            complement(914..>1107)
                     /locus_tag="VEA63_15625"
     CDS             complement(914..>1107)
                     /locus_tag="VEA63_15625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD85496.1"
                     /translation="TYRGPDGKQYVAILDGVGGWSGAVVAGDLDTRDGTAALGFVNAM
                     SDLKRATTQGGTLYVFRLP"
BASE COUNT          207 a          443 c          283 g          174 t
ORIGIN      
        1 ttccgcgccg ctgcctttca actccagaca cggcacgcca tactccgcca aaattttcgc
       61 gatctccgcc ccgcgtcgtt cgatcaccgc atccacctcc gccttcaacg ccgcatcgcc
      121 gcgccccacg cccatcgcga tcgcatacgt caacggcagc accggcccat cgcgttccgg
      181 cgtcactggc gtcacatcca acggcaccgc ttgccgtcgc gcgaaatatc ccgccattgg
      241 accccacgcg accgccacgt cgatttcgcc gcgcgccacc gcccgcatga tttccgccgg
      301 cggactctcc tgcgcataat cgccgtagag cgaataaccg cgcacgttct cgatcatccc
      361 gcgccgcgac aacgcatgcg ccggcggcgt gttgttgaaa tcgtctccga ccatctgcac
      421 cccgatcgtc aactcgtgca gtcgcggatc gtcgaacgat gtcacctcgc gccctgtccc
      481 cgcgcgctgc acgaaaacgt aagtggaacg ataatacggc cgcgtcgtca gcacgtggtc
      541 gatccccgcg gcgacgccga tcacgaggtc cgcacgcccc gcgccgatcg tctgccgaaa
      601 aaatccgcga cgttgcggcc accaaaaata ctcgagccgc gcgccaagct cgcgcgccac
      661 caactccgcg atgcgattct caaacccttc gcaccgctcg ttcgagaacg gcagattgtt
      721 cggatcggcc gccacccgca gcacgcgctc cggctccgcc gccaccatcc gcacgccgac
      781 aaaaatccac agacacaccg tcagaattcg caggcagggc accacctccg cgcggcccga
      841 tcgcgcacgc agccacccct gtgctcgcga aaattttccg cgcgccacgc agcggaagtc
      901 atcgcgcctc gatttacggc agtcgaaaaa catagagcgt tcctccctgc gtcgtggccc
      961 gtttcaaatc gctcatcgca ttcacgaacc ccagtgccgc cgtgccgtcg cgcgtatcaa
     1021 gatcgcccgc gacgaccgcg cccgaccagc cgccgacgcc gtcgaggatc gccacgtact
     1081 gcttgccgtc ggggccgcgg taggtga
//
LOCUS       DASPLT010001531         2039 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_120075, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001531 DASPLT010000000
VERSION     DASPLT010001531.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 2039)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2039)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2039
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_120075"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..258)
                     /locus_tag="VEA63_15630"
     CDS             complement(<1..258)
                     /locus_tag="VEA63_15630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007362692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HYD85497.1"
                     /translation="MTLFLLVLLAALIFEYINGFHDTANAIATVVSTKVLTPRQAIVW
                     AAFWNLIGALMGTAVASTIGKGLVDTQFVTMATVLAALLSAI"
     gene            complement(329..967)
                     /locus_tag="VEA63_15635"
     CDS             complement(329..967)
                     /locus_tag="VEA63_15635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007362693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 family protein"
                     /protein_id="HYD85498.1"
                     /translation="MLWLKKLFGKDDKFFDLLEAGAEEAKTSVNLLSRYLQTLSNGGP
                     RANLDEFVQSRRKEKRIRHQMTEELSKTFVTPLEREDIEQLSFALYRIPKAIEKIVER
                     LSIYPGRVPHTAFQRQAELLNLAAEAVVFMVKELRGGTSIEKVREANDRLQFAEGEAD
                     KVMLGLLKELYHGPYDAKELVILQELYEMVEQAVDRCRNAGNIVVQIVLKHS"
     gene            complement(1043..1831)
                     /locus_tag="VEA63_15640"
     CDS             complement(1043..1831)
                     /locus_tag="VEA63_15640"
                     /inference="COORDINATES: protein motif:HMM:NF014724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ElyC/SanA/YdcF family protein"
                     /protein_id="HYD85499.1"
                     /translation="MGFALKKFISFWLMPLPFCGLLLVVGLCLLLFSKRRRLARILLV
                     TGTALLLLFSNKRVSMRLIRPLETLYPPVPELVAGETLSAPLANLRYIVVLGGGHGPS
                     PDFSAVNQLSTSARGRLMEALRLARALPETQLVFCGHAGEGRKSHAAVLAEAAISLGF
                     EPARMVRLENPRDTEDESIAIAQLVGSQRFALVTSAWHLRRAVALMRHHGLDPLPVPA
                     DFASRHPEQTTLSDYTWDTESLGRSTWAVREQIGYLWSRLRGKI"
BASE COUNT          406 a          564 c          684 g          385 t
ORIGIN      
        1 gatcgcgctg agcaaggcgg cgagcacggt cgccatggtc acgaattggg tatcgacgag
       61 acctttgccg atggtcgagg cgacggcggt gcccatgagc gcaccgatga gattccaaaa
      121 ggcggcccaa acgattgcct ggcgcggcgt caggaccttc gtggagacga cggtcgcgat
      181 ggcgttggcg gtgtcgtgaa agccgttgat gtattcaaaa atcaacgccg cgagcaggac
      241 gagcaggaac agagtcatga ggcagtcggt ggtagcggaa accgtgaggt tttcgcgaaa
      301 cgtccaggcg ttccgctacg cggcgagatc acgaatgctt gaggacgatt tgaacgacga
      361 tgttgcccgc gttgcgacag cggtcgacgg cttgttccac catctcgtag agctcctgca
      421 aaatgacgag ctccttggcg tcgtaggggc cgtggtagag ttccttgagc aacccgagca
      481 tcaccttgtc ggcttcgccc tcggcgaatt gcaggcgatc gttggcctcg cggacttttt
      541 cgatgctcgt gccgccgcgc agttctttca ccatgaacac gaccgcttcg gccgcgagat
      601 tgagcaactc ggcctggcgc tggaacgcgg tgtgcggcac gcggccggga tagatcgaga
      661 ggcgttcgac gattttctcg atcgcctttg gaatgcgata aagcgcgaaa gaaagctgct
      721 cgatgtcctc gcgttcgagc ggggtgacga aggttttgct gagctcctcc gtcatttgat
      781 ggcgaatacg tttctccttg cgacggctct gcacgaactc gtcgagattc gcccgcggcc
      841 cgccattgct cagcgtttga aggtagcggg agaggaggtt gacgctcgtc ttcgcctctt
      901 ctgcgccggc ctcgaggaga tcgaagaact tgtcgtcttt gccaaacagc ttcttgagcc
      961 acagcatgat gtcgcctgag tagctgcgaa cgtcacgatt gcgaaacaat tctacgccga
     1021 attcctgtcg gcgatgcggc cgctaaattt ttccgcgcaa ccggctccaa aggtagccga
     1081 tttgctcgcg aaccgcccag gtgctgcggc ccagcgattc ggtgtcccac gtgtaatcgc
     1141 tcaaggtcgt ttgctcggga tgacgcgatg cgaaatccgc cggcacgggc aacggatcga
     1201 gtccgtgatg acgcatcaac gcgaccgcgc gacgcaggtg ccacgccgag gtgacgagcg
     1261 cgaagcgctg gctgccgaca agttgggcga tcgcgatgga ctcgtcctcg gtatcgcgcg
     1321 gattttccaa gcgcaccatg cgcgccggtt cgaagccgag cgagatcgcg gcttcggcga
     1381 gaacggcggc gtggcttttt cggccttcgc cggcgtgacc gcagaaaacg agttgagttt
     1441 cggggagcgc gcgagcgagt cgcagcgctt ccatcaagcg gccgcgcgcc gatgtgctca
     1501 actggttcac ggcggaaaaa tccggagacg ggccgtgacc gccgccgagc acgacgatgt
     1561 agcgtagatt ggcgagcggc gcggacaggg tttcgcccgc gacgagttcg ggcacgggcg
     1621 gatagagggt ttcgagcgga cggatcagcc gcatgctcac ccttttgttg ctaaacagga
     1681 gcaacagcgc cgtgccggtc acgagcaaga tgcgcgcgag acggcggcgc ttcgaaaaga
     1741 gcaggaggca gagaccgacg acaaggagca ggccgcagaa cggcagcggc atcagccaga
     1801 acgaaataaa ttttttgagt gcgaagccca cgatgcggtg acgatgaggg ttattgaaga
     1861 ggggaaacgt gcgggtggcg caaggatggt tcgcgttgag tggacggctg accgggatcg
     1921 atcgaccgac taggcaggca cggaggcctg gcgccacggc gcaaataaat ttgctccggc
     1981 ggttgcgggc tgtttcggtg cgcggcttca tgaaactctg gtcgcagtat tttatcccc
//
LOCUS       DASPLT010001532         1407 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_242712, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001532 DASPLT010000000
VERSION     DASPLT010001532.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1407)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1407)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1407
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_242712"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            103..1308
                     /locus_tag="VEA63_15645"
     CDS             103..1308
                     /locus_tag="VEA63_15645"
                     /inference="COORDINATES: protein motif:HMM:NF025034.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartyl protease family protein"
                     /protein_id="HYD85500.1"
                     /translation="MKLRPSTLRPVVRVFLALALVAVVSGCQLFRRELPRPGRTELDS
                     ELVIVPAKAMSNYLVVEAKWDKRGPYHFLIDTGASVTLVSPEFAARYAAKNAPPATTP
                     LVRVKSAAGDTALLTATTLRRIELGDAVFENVQALVYDCAAISAHLGIQIDGILGFPL
                     FRETILTLDYPHSRVLLQRRSAKSALPGVQIPFNNERKTPIISLRLGDRSFIALIDSG
                     SDAFLSLNPFGLDPVFAQPPRPGATIATLAGDREQQIGRLAETLFIGDYAFRQPIVDL
                     TDELTSIGGGLLKHFTVSFDQEHSRVTFHRNTLEPQPPQPLRNAGVSFNKTPAYWRVA
                     SVVPNSPAAAAGVQTGDLVTRINGEPVAQWDITRYQQLVLTAREIAFTFLNGTEETEK
                     RIPVFDLVP"
BASE COUNT          235 a          531 c          371 g          270 t
ORIGIN      
        1 caccgttttc tgggtcttcg gctccgcgag catcttcgcc gtcggcgcgg ttttcgcact
       61 gggtgcactg tggctcagcc gcaccttgcc gcctcccgtg aaatgaaact ccgcccgtcc
      121 acgctccggc ccgtcgttcg cgttttcctc gcgctcgctc tcgtcgcagt cgtgtccggc
      181 tgccagcttt tccgccgcga actcccccgg cccggtcgca ccgaactcga ttcggagctc
      241 gtcatcgttc ccgccaaggc gatgtcgaac tacctcgtgg tcgaggcgaa gtgggacaaa
      301 cgcggccctt atcacttcct catcgacacc ggcgcctccg tcaccctcgt gtcgcccgag
      361 ttcgccgcgc gctacgccgc aaaaaacgcc ccgcccgcca ccacgccgct cgtgcgcgtg
      421 aaatccgccg ccggcgatac cgcgctgctc accgcgacaa cgcttcgccg catcgagctc
      481 ggcgatgccg tgttcgaaaa cgtgcaggcg ctcgtctacg attgcgccgc catctccgct
      541 cacctcggca tccagatcga cggcattctc ggctttcccc ttttccgcga aacgattctc
      601 acgctcgact acccgcactc gcgcgtgctg ctccagcgcc gctccgcgaa atccgcgctt
      661 ccgggcgtcc agattccgtt caacaacgaa cgcaagacgc cgatcatctc gctccgcctc
      721 ggcgaccgat cgttcatcgc gctgatcgat tccggcagcg acgcctttct cagccttaat
      781 cctttcggcc tcgacccggt gttcgcccag ccgccacgcc ccggcgccac catcgccacg
      841 ctcgccggcg atcgcgagca acagatcggc cgcctcgcgg agacgctctt catcggcgac
      901 tacgcgtttc gccagccgat cgtcgacctc accgacgagc tcacgtcgat cggcggcgga
      961 ttgctcaagc atttcaccgt gtccttcgat caggagcaca gccgcgtgac gtttcatcgc
     1021 aatacgctcg agccgcaacc gccgcagccc ttgcgcaacg ccggcgtgag cttcaacaaa
     1081 acgcccgctt actggcgcgt cgccagtgtc gtcccgaatt ctcccgctgc ggctgcgggc
     1141 gtgcaaaccg gcgatctcgt cacgcgcatc aacggcgagc ccgtcgcgca atgggacatc
     1201 acgcgctacc agcagctcgt cctcaccgcg cgcgaaatcg cattcacgtt tctgaacggc
     1261 accgaggaaa ccgagaaacg catccccgtc ttcgacctgg tgccataatc tttctctttc
     1321 ctctttatct ttctctttct cggatcgagg gcggcgcgga gtggcgtcgg cccgagaaag
     1381 agaaagataa agaggaaaga gaaagat
//
LOCUS       DASPLT010001533         1905 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_58083, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001533 DASPLT010000000
VERSION     DASPLT010001533.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1905)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1905)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1905
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_58083"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1116
                     /locus_tag="VEA63_15650"
     CDS             <1..1116
                     /locus_tag="VEA63_15650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD85501.1"
                     /translation="NGSAIEGWFTMTGDGTAKIDFVPNDGFARMQIDGTQDRHNVWWT
                     LIKRDITADLDLAKLADPAYELRVEARVRASHAPRRVNFMINTQRTTDFHQHLREYDL
                     ADTSDWHTISMTTQDFDARPGDTVFVQLAATDWGSGKYHLDVDYYRADVVRRDGAKPD
                     VGEPLVYHPPIPDLKTFEHHLAVTHDSVVHVDFPDVNFNDWHVTDASGPARVLTVDAR
                     QSAILRWNFGEYRARAVDGAGILELTTSSVAKGGDYVRHFGQDLGIEFGKLRVIEILG
                     GDPAWDQQTVTYRSLLREDAATDAFNGQMITDIELTEDPHGKSYATLPRPVMQRLLEG
                     RTKGIILQPLGALAASVYASESAAEKRPTLHFNTRAK"
     gene            1215..>1905
                     /locus_tag="VEA63_15655"
     CDS             1215..>1905
                     /locus_tag="VEA63_15655"
                     /inference="COORDINATES: protein motif:HMM:NF018178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HYD85502.1"
                     /translation="MLLRRPIRSPFAALFALTFFASAVATGSAAASNHFERLFNGENL
                     DGWVVPAGDNGHWKVVDGVIDYDAQSEATADKNLWSEREFGDCVLRVEWRIKETPYVN
                     RHVPYILPDGTHARDITGKTLLLSLPDSDSGILFRGDVKNQVNIWCWPIGSGELYGYR
                     TDPNSSAETRAAVTPRTQADKPVGEWNLFEITLRGDRISVLLNSVLVIDQAQLPGIAR
                     RGRIGLQHHGSM"
BASE COUNT          388 a          648 c          531 g          338 t
ORIGIN      
        1 aacggttccg ccattgaagg ctggttcacg atgacgggcg acggcacggc gaagatcgac
       61 ttcgtgccga acgacggttt tgcgcgcatg cagatcgatg gcacgcagga tcggcacaac
      121 gtctggtgga cgctgatcaa acgcgacatc accgccgacc tcgacctcgc gaagctcgcc
      181 gatcctgctt acgaactccg agtcgaagcc cgcgtgcgcg cgagccatgc accgcgacgc
      241 gtcaacttta tgatcaacac gcagcgaacg accgactttc accagcacct gcgcgaatac
      301 gacctggccg atacttccga ctggcacacc atcagcatga cgacgcagga cttcgacgct
      361 cgtcccggtg acaccgtttt cgtgcagctc gccgcgactg attggggttc cggcaagtat
      421 caccttgatg tcgattatta ccgcgccgat gtcgtccgcc gcgacggagc aaaacccgac
      481 gttggcgaac cgctggttta ccatccaccg attccggatc taaagacgtt cgaacatcat
      541 ctcgccgtca cgcacgacag tgtcgttcac gtcgatttcc ccgacgtcaa cttcaacgac
      601 tggcacgtca cggacgccag cggccccgcg cgtgtcctca ccgtggatgc acgccagtcg
      661 gcgattctgc ggtggaactt cggtgaatat cgcgcgcgcg ccgtcgatgg agccggcatc
      721 ctggagttga cgacgtcgtc ggtcgcaaaa ggcggcgact acgtccgcca cttcggccag
      781 gatctcggga tcgagttcgg caaacttcgc gtgatcgaaa ttctcggcgg cgatcccgcg
      841 tgggatcaac aaacggtgac ctatcgcagc ctcctgcgtg aagacgccgc cacggacgca
      901 ttcaacggcc agatgatcac cgacatcgag ttaacggaag acccgcacgg gaaaagttac
      961 gcgacacttc cccgtcctgt catgcaacgg ctgctcgaag gccgcacgaa gggaatcatc
     1021 ctgcaacccc tcggggcact cgccgcctcc gtttacgcgt cagagagcgc cgcggaaaaa
     1081 cgtcccacgc tgcacttcaa cacgcgtgcc aaataaaaag cagctccgcg cgtgagcgca
     1141 gggattttcg agcccagcgc ccagcccgca cacctcttgg ttgaaagcgg tgcgaaaccg
     1201 gcgacgctct ctgcatgctc ttgcgtcgcc ccatccgctc acccttcgcc gccctctttg
     1261 ctctcacgtt ttttgcgagt gccgtcgcca ccgggagcgc tgccgccagc aatcatttcg
     1321 agcgcttgtt caacggcgaa aacctcgacg gctgggtcgt tcccgccggc gacaacggcc
     1381 actggaaagt cgtcgacggc gtgatcgact acgacgcgca gagcgaggcg accgccgaca
     1441 agaacctttg gagcgagcgc gagtttggcg actgcgtcct gcgcgtcgaa tggcggatca
     1501 aggagacgcc ctacgtaaac cgacacgtgc cttacatcct ccccgacggc acgcatgcgc
     1561 gcgacattac cgggaagacg ttgctcctct cgctgcccga ttccgactcc ggcattctct
     1621 ttcgcggcga cgtcaaaaac caagtgaaca tctggtgctg gccgattggc tccggcgaac
     1681 tctacggcta tcgcaccgat cccaactcca gcgccgagac gcgcgctgcc gtgactccgc
     1741 gcacgcaagc cgacaaacct gtcggcgaat ggaacctgtt cgaaatcacg ctgcgcggcg
     1801 atcgcataag cgtgctcctc aacagcgtcc tcgtcatcga tcaagcccag cttcccggga
     1861 tcgcccgacg cggtcgcatc ggtctgcaac atcatggctc catgc
//
LOCUS       DASPLT010001534         2239 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_242792, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001534 DASPLT010000000
VERSION     DASPLT010001534.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 2239)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2239)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2239
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_242792"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..756
                     /locus_tag="VEA63_15660"
     CDS             <1..756
                     /locus_tag="VEA63_15660"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HYD85503.1"
                     /translation="HALGLAFHGGGLGVKVTVVMPVSAPAVKVERCRGLGAEVVLHGE
                     NFEAAERYARERAADEGASYVHPFDDVRVIAGQGTLALEWLEQAPELDTIVVPVGGGG
                     LLAGVATVVKALRPDVRVVAVEPENAAGFLAACLRGAPSRAALLPTLADGLAVARVAE
                     TTFTLAAPLVDDVVTVSEAEIERAIGVLARENGIVAEGAAATGVAALLSGKIGRTRAV
                     GLAITGRNIDARVHDRVLARTAGERRHALAHAA"
     gene            1112..2068
                     /locus_tag="VEA63_15665"
     CDS             1112..2068
                     /locus_tag="VEA63_15665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HYD85504.1"
                     /translation="MQSPTLLVLAAGMGSRYGGLKQIDPVGPSGETVLDYAVFDALRT
                     GFGRVVFVIRREFEAVFREQVGAKYTGRVEVDYVFQALDALPEGFAVPAGREKPWGTG
                     HAVWCAREAVNGNFAVINADDFYGADSFRQLAGFLTAAEARRDSAGAAPAEFAMVGFR
                     LAKTLSEHGAVSRGVCVTANDGRLKSITERTGILAKEVGAGRVFSGEEIVSMNCWGFT
                     TDLFAGLDARFREFLETGRAESGRKASPSVGEFYLPAAVSEMLARGEAVVEVLPTESA
                     WFGVTYREDKPRVVAAIAELVRAGAYPAKLFSNRKETTNGHE"
BASE COUNT          361 a          540 c          907 g          431 t
ORIGIN      
        1 cacgcgctcg ggctcgcgtt tcatggcggc gggttggggg tgaaggtgac ggtggtgatg
       61 ccggtgagtg cgccggcggt gaaagtggag cggtgtcgcg ggctcggcgc ggaggtggtg
      121 ttgcacgggg aaaatttcga agcggcggag cggtatgcgc gagagcgggc ggcggacgaa
      181 ggcgcgagtt atgtgcatcc gttcgacgat gtgcgcgtga tcgcgggaca ggggacgctc
      241 gcgctggagt ggctcgagca ggcgccggag ctggacacga tcgtggtgcc cgtgggcggc
      301 gggggattgc tggcgggtgt ggcgacggtg gtgaaggcgt tgcggccgga cgtgcgcgtg
      361 gtggcggtgg agccggagaa cgcggcgggg tttctcgcgg cgtgtttgcg cggagcaccg
      421 agtcgggcgg cgctgctacc gaccttggcg gacgggctcg cggtcgcgcg ggtggccgag
      481 acgacgttca cgttggccgc gccgctggtc gacgacgtgg tgacggtaag cgaggcggag
      541 atcgagcgcg cgatcggcgt gctcgcgcgc gagaatggca tcgtggccga aggcgcggcg
      601 gccacgggcg tcgccgcgtt gttgagcggc aagatcgggc gaacgcgggc ggtaggcctg
      661 gcgatcacgg ggcgcaacat cgacgcgcgt gtgcatgacc gcgtgctcgc gcgaacagcg
      721 ggggaacgac ggcacgcgct ggcgcacgcg gcgtgagtgt ggcgggaaca ggagtccgct
      781 attaggaaaa gagtgggagg agggagaggc gtagatcagt ttttgagcgg tcgcggagcg
      841 aactcacgag aatagccgtg gctttcaagc caagggtaac aggtccgtct cggtgaggcg
      901 tcgcgtagcg acgtttggtg gccgcgtcat caagcgtcgc tacgcgacgc gtcggtccga
      961 ctgatcatca tccgtggctt gaaagccacg gctattctcg gtcctcgctc tgcgaggaac
     1021 ctgattcctc ttttctcatt tgcgaccgtg aggggaaggc ccgaaagcgg cgccttgcgc
     1081 gtggacgggt ggaggcttct gctcgcgggc gatgcaatct cccacgcttc ttgtcctcgc
     1141 ggccggcatg ggctcgcgtt acggcgggct caagcagatc gatccggttg ggccgtcggg
     1201 cgagacggtg ctcgactacg cggtgttcga tgcgctgcgc actggttttg gacgcgtggt
     1261 gtttgtgatt cggcgggaat tcgaagcggt gtttcgcgag caggtcggtg cgaaatacac
     1321 cggacgtgtg gaggttgatt acgtgtttca agcgcttgac gcgctgccgg agggtttcgc
     1381 tgtgcctgca gggcgcgaga aaccgtgggg gacgggtcat gcggtgtggt gcgcgcggga
     1441 ggcggtgaac ggaaatttcg cagtgatcaa tgcggatgat ttttacggtg ctgattcgtt
     1501 tcggcagctc gcggggtttt tgacggcggc cgaggcgagg cgtgacagcg caggcgccgc
     1561 gccggcggag tttgcgatgg tggggtttcg gctggcgaag acgttgtcgg agcacggagc
     1621 ggtgtcgcgc ggcgtttgcg tgacagcgaa cgacgggcgt ctgaaatcga tcacggagcg
     1681 aacggggatt ctggcgaagg aggtgggcgc ggggcgggtg ttttctggag aagagatcgt
     1741 ttcgatgaac tgctgggggt tcacgaccga tttgttcgcg gggctggatg cgcggtttcg
     1801 agagtttttg gagacggggc gcgcggagtc ggggcgtaaa gcctctccct ccgtggggga
     1861 attttatttg ccggcggcgg tgtcggagat gctcgcgcgc ggggaggcgg tggtcgaggt
     1921 gttgccgacg gagagcgcgt ggttcggcgt gacgtatcgc gaggacaagc cgcgggtggt
     1981 cgcggcaatc gcggaactcg tgcgggcggg ggcgtatccg gccaaattgt tctcaaacag
     2041 aaaagaaacc acgaatggac acgaatgaac acgaatgaaa ccgaactcga atgcgtagtc
     2101 gtttcgagcg ccgaagggcg atgcaacgat cgcctgagag ctttcctttg gctcgtctga
     2161 attcgtgttc attcgtgtcc attcgtggtt caaatgaatt agtctcgctg agatgatgct
     2221 gcgttcattg gagaaagag
//
LOCUS       DASPLT010001535         1080 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_305791, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001535 DASPLT010000000
VERSION     DASPLT010001535.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1080)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1080)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1080
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_305791"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..576
                     /locus_tag="VEA63_15670"
     CDS             <1..576
                     /locus_tag="VEA63_15670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD85505.1"
                     /translation="SQRLGASALADGSKDAAILVTLDPGVYSAQVDAQGGAPGIVLVE
                     AYDADDNITAALPRRLLNLSSRSFAGNGDETLIAGFVVDGNSPKQVLIRAVGPALAAA
                     GITDHNPDPNLQLFDGNGTRVADNDDWAYSNQTDILPSIFTKVGAGQLADGSYDSALL
                     ITLRPGVYSAQANGRSGETGVIVIEVYEVPQ"
     gene            689..>1080
                     /locus_tag="VEA63_15675"
     CDS             689..>1080
                     /locus_tag="VEA63_15675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD85506.1"
                     /translation="MPSRVHLFWFVIPTLALSLRGQTLPKDSPFLAPPSETAPAHVDS
                     AAGYQLSGMTVVGTDTLLSITRENDQRSQWIAVGQTLDEISVVSYDAAKDQAVIRVGE
                     KEHTLTLRKPSIAAATVSPLIPPNPAAAG"
BASE COUNT          198 a          415 c          270 g          197 t
ORIGIN      
        1 tcccaacgtc tcggtgcgtc cgccttggcc gacggcagca aggacgccgc cattctcgtt
       61 acactcgatc ccggcgtcta ttccgcccaa gtcgacgccc aaggcggcgc ccccggcatc
      121 gtgctcgtcg aagcctacga cgccgatgac aacataaccg ccgcgcttcc ccgccgcctg
      181 ctcaacctct cttcgcgcag cttcgccggc aacggcgacg aaacgttgat cgcgggcttc
      241 gtcgtcgacg gcaacagccc caaacaggtt ttgattcgcg ccgtcggtcc cgcgctcgcc
      301 gcagccggca tcaccgatca caatcccgat cccaatctcc agctcttcga cggcaacggc
      361 actcgcgtcg ccgacaacga cgactgggcg tattcgaacc agaccgatat tcttccatcc
      421 atcttcacca aggtcggcgc cggccaactt gccgacggca gctacgacag cgccttgctc
      481 atcactcttc gccccggcgt ctactccgcc caggcgaacg gacgctcggg agaaaccggc
      541 gtgatcgtca tcgaggttta cgaggtgccg caataaacgg cgaacgaacg cttcgcctgc
      601 ggacgccacg cctctccttc gccatcgtcc cgcgcctgca gccggagcct tgctccatcg
      661 atttctttcg accacgtttc tccaccgcat gccttcgcgc gttcatctct tctggttcgt
      721 gattcctacg ctggctctct cgcttcgcgg ccagactctg ccgaaagatt ccccctttct
      781 tgctccgccg agcgagacag cgcccgcgca cgtcgactcc gcggccggtt atcaactctc
      841 cggcatgacc gtcgtcggaa cagacacctt gctcagcatc acccgcgaga acgaccaacg
      901 cagtcagtgg atcgccgtcg gccaaacgct cgacgaaatc agcgtcgtca gttacgatgc
      961 cgcgaaagat caagcagtca ttcgcgtcgg cgaaaaggaa cacacgctca ccctgcgcaa
     1021 acctagcatc gccgccgcca ccgtttcgcc gcttatcccg cccaatcccg ccgcagccgg
//
LOCUS       DASPLT010001536         1564 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_183158, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001536 DASPLT010000000
VERSION     DASPLT010001536.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1564)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1564)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1564
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_183158"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1519)
                     /locus_tag="VEA63_15680"
     CDS             complement(<1..1519)
                     /locus_tag="VEA63_15680"
                     /inference="COORDINATES: protein motif:HMM:NF019229.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRTAC1 family protein"
                     /protein_id="HYD85507.1"
                     /translation="MNNDGRLDLYVCRVAAPNLLYVNQGDGTFAERANEAGIAVTDAS
                     VMGAFCDYDRDGWLDLYLVTNLLDYENKPQGERDRLFRNRGDGTFEDVTERAGIFGEG
                     QKHAAVWWDHDGDGWEDVYVTYDYGWPDQLWRNNGDGTFANVLGGALPYIPRYSMGAD
                     IGDVNNDGRMDLFVLDMLPTTRERDQRAMIDARAHIPDLADPAMTPQYMRNMLFLNTG
                     TALFQEGAFLAGIAATDWSWSARLEDFDNDGRVDLFVTNGTIREFFDGDLRKRIENLP
                     PAERKRVFKNSPVLRERNLAYRNLGDVHFEKSSAAWGLDELGVSFGAATGDLDGDGDL
                     DLLYSNFDGDVTLCRNEGEGGNRVVIALRGEAANRFGVGATVRIETAAGPQVRTLGIA
                     RGYLSSSEPVLHFGLGTAERIERLTVTWPGGREQTFTGLAANRRYVVTEATHGEPPAK
                     PEKPPGQFVEVGAFLGLNVHPPLTSFDELATQPLLAFRQNQIGPGIAVGDMNGDRV"
BASE COUNT          272 a          585 c          424 g          283 t
ORIGIN      
        1 ccacccggtc gccgttcata tcacccactg cgatgcccgg tccgatttga ttttgtcgaa
       61 acgccagcaa gggctgcgtc gccagttcgt cgaacgacgt cagcggcggg tgcacgttca
      121 acccgagaaa cgctcccacc tcgacgaact gccccggcgg tttctccggt ttcgccggcg
      181 gctcgccgtg cgtcgcctcg gtaacgacat agcggcggtt cgccgccagc ccggtgaacg
      241 tctgctcgcg tccgcccggc caggtcaccg tcagccgctc gattcgctcc gcggttccca
      301 gtccaaaatg cagcaccggc tcgctgctcg agagatagcc gcgcgcgatt cccaacgtgc
      361 gcacctgcgg ccccgccgcc gtctcgattc gcaccgtcgc cccgacgcca aaccggttcg
      421 ccgcttcgcc gcgcaacgcg atgaccacgc gattgccgcc ctctccttca ttgcggcaaa
      481 gcgtcacgtc gccgtcgaag ttcgaataca gcagatccaa gtcgccgtcg ccgtcgagat
      541 cgcccgtcgc cgcgccgaag ctcacgccga gctcgtccag cccccacgcc gcactgcttt
      601 tttcgaaatg cacgtcacca agattccgat acgccaggtt gcgctcgcgc agcaccggcg
      661 aattcttgaa cacccgcttc cgctccgccg gcggcaggtt ctcgatccgc ttgcgcaaat
      721 ccccatcgaa aaactcgcgg atcgtgccgt tcgtcacgaa cagatcgacg cggccgtcgt
      781 tgtcgaagtc ctccagccgc gccgaccacg accagtccgt cgccgcgatc cccgccagaa
      841 acgcgccttc ctgaaacagc gccgtgcccg tgttcaaaaa aagcatgttc cgcatgtatt
      901 gcggcgtcat cgccgggtcc gccaaatccg ggatgtgcgc gcgcgcatcg atcatcgcgc
      961 gctgatctcg ctctcgcgtc gtcggcagca tgtcgagaac gaacaggtcc atgcgtccgt
     1021 cgttgttcac gtcgccgata tcggcgccca tcgagtagcg cgggatgtaa ggcagcgctc
     1081 cgccgagcac attcgcgaac gtgccgtcgc cgttgttgcg ccacaactga tccggccagc
     1141 cgtaatcgta agtgacgtaa acatcctccc agccatcgcc gtcgtgatcc caccacactg
     1201 ccgcatgctt ttgcccctcg ccaaaaatcc cggcgcgctc cgtcacgtcc tcgaacgtgc
     1261 cgtcgccgcg attccggaaa agccggtcgc gctcgccctg cggcttgttt tcgtaatcga
     1321 ggagattcgt cacgagataa agatcgagcc agccatcgcg atcgtagtcg caaaacgcgc
     1381 ccatcacgct cgcatccgtc accgcgattc ccgcctcgtt cgcacgctcc gcaaacgtgc
     1441 cgtcgccctg gttcacgtag agtagatttg gcgctgcgac ccggcacacg tagagatcga
     1501 gccggccatc gttgttcacg tctgcaaaag cgacgcccgt cttccacgcg cccgccgggc
     1561 cgcc
//
LOCUS       DASPLT010001537         1556 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_305802, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001537 DASPLT010000000
VERSION     DASPLT010001537.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1556)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1556)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1556
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_305802"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(167..1240)
                     /locus_tag="VEA63_15685"
     CDS             complement(167..1240)
                     /locus_tag="VEA63_15685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HYD85508.1"
                     /translation="MKLLITGVCGFVGSTLARALAESSAGYEIVGFDNFIRPGSETNR
                     DALKHLGVKLHHGDLRAASDLETLPAVDWVLDAAANPSVLAGVDGRTSSRQLVEHNLS
                     GTVNMLEFCKQHRAGFILLSTSRVYSIPPLSSLAVEAVDHAFRPAANAALPPGLTASG
                     LDETFSTAAPVSLYGVTKLASEALALEYGETFNLPVFINRCGVLAGAGQFGRPDQGIF
                     AFWLNSHLRRRPLAYIGFGGHGHQVRDCLHPRDLAPLLAQQFTAPKLAATDRIANFSG
                     GAASAMSLRQLTAWCDQRFGAHPVSANPTPRPFDIPWIVLDHAKATRLWNWRPQTPTS
                     AILDEIATHAQAHPHWLDLSAPL"
BASE COUNT          280 a          352 c          665 g          259 t
ORIGIN      
        1 gcggctgccg tcgtcgacga cgacgatttc gtgcgggatg ttttccgcgg tgaaggtgcg
       61 ataaatgtct tcgacggtgg atgggaggga gtcttcctcg tcgcgggcgg gaatgatgac
      121 ggagtaaagc tggagcggag cggcggcgga catgaagtgg gaaaattcag agaggcgcgg
      181 agagatcaag ccagtgcggg tgggcctgcg cgtgggtggc gatctcgtcg aggatcgcgc
      241 tcgtgggagt ttgcgggcgc cagttccaga gacgcgtggc tttggcgtga tcgaggacga
      301 tccacggaat gtcgaagggg cgtggcgtgg ggttggcgga gacggggtgg gcgccgaagc
      361 gttgatcgca ccaggcggtg agttggcgaa gcgacatcgc ggacgcggcg ccgccggaga
      421 agttcgcgat gcgatcggtg gcggcgagtt tcggcgcggt gaattgttgc gcgagcagcg
      481 gcgcgagatc gcgcgggtga agacaatcgc ggacctgatg gccgtgtccg ccgaagccga
      541 tgtaggcgag cggacggcgg cggagatgac tgttgagcca gaacgcgaag atgccctggt
      601 cgggccggcc gaactggccg gcgccggcga ggacgccgca gcggttgatg aagacgggga
      661 ggttgaacgt ctcgccgtat tcgagcgcga gcgcttcgga ggcgagtttg gtgacgccgt
      721 agagcgagac tggcgcggcg gtggaaaacg tttcgtcgag gccggacgcg gtgaggccgg
      781 gcgggagcgc ggcgttggcg gcggggcgga acgcgtggtc gacggcttcg acggcgagtg
      841 aggagagcgg cgggatggag tagacgcggc tggtgctgag caggatgaaa ccggcgcggt
      901 gctgtttgca gaactcgagc atgttcaccg tgccggagag attgtgctca acgagctggc
      961 gggaactggt gcggccgtcg acgccggcga ggacgctcgg gttggcggcg gcgtcgagga
     1021 cccagtcgac ggcgggaagc gtttcgaggt cgctggcggc gcggaggtcg ccgtggtgga
     1081 gtttgacgcc gaggtgtttg agagcgtcgc ggttcgtttc gcttccgggc cggatgaagt
     1141 tgtcgaagcc gacgatctcg tagccggcgc tggattcggc gagcgcgcgg gccagcgtgc
     1201 tgccaacgaa gccgcacacg ccggtgatga gaagtttcat gcggcgcgaa acaagcaggg
     1261 gcgcggcggt ggcggaaggc gaaacttagc tgggaccatg caattgaacc acggatggac
     1321 acagatggac acagatgcga tttcggtttt gaacgggatg gattcggcgt tgcggcgggc
     1381 ggggatggga tgcaaacaac ggcggtgaaa cttggagttg gaaaaagaaa agaatggagg
     1441 gcgatctggt tcgcgatggt ggcgttggcg atggtggtgg acgcggcgga ggtgcgaagg
     1501 gtcgaaggaa ttgggatcga gctgattcgc gtgccagtgg ggacgtttgt gatggg
//
LOCUS       DASPLT010001538         1057 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_18992, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001538 DASPLT010000000
VERSION     DASPLT010001538.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1057)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1057)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1057
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_18992"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1057
                     /locus_tag="VEA63_15690"
     CDS             <1..>1057
                     /locus_tag="VEA63_15690"
                     /inference="COORDINATES: protein motif:HMM:NF037085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MBG domain-containing protein"
                     /protein_id="HYD85509.1"
                     /translation="ATQTGEFNIALTAANGSGTGNASTLVLTIAKAPLAVTANNASRE
                     FGDANPVFTVSYTGLLGGDTAGSLTTAPAATVTTDVNTVPGTYAITPAGGVSDNYAFT
                     YVNGVLTITPAAQTITFSSIGERVIGDEVSLSATASSGLVVEFSIVSGPATISGSRLS
                     TTGVGTVIVRASQPGNSNYLSAANVDRTFSVHIGAPTVTTPTKIPTPPTGGQAELEVV
                     GSNANVDYQWQRNGADLPGATGPSLTIDDVQPPTAGLYTYTASVPGGGAGATSDPVIV
                     GVTTDKKVDGSGSEVNADVQHPNGNTYDQVLLEGNGAVVTANPAQITRVSFIDLSDDI
                     VQVEFTGAGSLAVVIDGQ"
BASE COUNT          198 a          348 c          334 g          177 t
ORIGIN      
        1 tgcgacgcag acgggcgagt tcaacatcgc gttgaccgcg gcgaatggca gcggcaccgg
       61 caacgcttcg acgctcgtcc tcacgatcgc gaaagcaccg ctggcggtga ccgccaacaa
      121 cgcgtcgcgt gagttcggcg acgcgaatcc agtcttcacg gtcagctaca ccggcctgct
      181 cggtggcgac acggcgggtt cgttgacgac ggcgccggcc gccaccgtga cgacggacgt
      241 taacaccgtg cccggcacgt atgcaatcac gccggcgggt ggtgtttccg ataactatgc
      301 gttcacctac gtgaacggcg ttctcaccat cacgcccgcg gcgcaaacga tcacgttttc
      361 gtcgatcggc gagcgcgtga tcggagatga agtgagcctt tccgccaccg cgagctctgg
      421 tctggtggtg gagttctcca tcgtcagcgg acctgcgacg atctcaggtt ccaggctctc
      481 gacgacggga gtcgggacgg tgatcgtgcg cgcgtcccag cccggaaatt caaactatct
      541 cagcgccgcc aacgtcgatc gcaccttcag cgtgcacatc ggtgcaccga ccgtcacgac
      601 cccgacgaag attccgacgc cgccgaccgg tggccaggcc gagctcgaag tcgtgggctc
      661 caacgcgaac gtcgattatc aatggcaacg caacggcgcc gatcttcctg gcgccaccgg
      721 tccgtcgctc accatcgatg acgtgcagcc gccgaccgcc ggcctctaca cctacacggc
      781 cagcgtgccc ggcggcggtg ccggtgcgac gagcgatccg gtgatcgttg gggtcacgac
      841 cgacaagaag gtcgacggca gcggttcgga agtgaatgcc gatgtgcaac acccgaacgg
      901 caacacctac gatcaggttc tgctcgaagg aaacggcgca gttgtgaccg cgaatcccgc
      961 gcagatcacg cgcgtgtcgt tcatcgattt gtcggatgac atcgtgcagg tggagttcac
     1021 cggcgccgga tcgctcgcgg tcgtgatcga cggccag
//
LOCUS       DASPLT010001539         1279 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_162227, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001539 DASPLT010000000
VERSION     DASPLT010001539.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1279)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1279)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1279
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_162227"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..215)
                     /locus_tag="VEA63_15695"
     CDS             complement(<1..215)
                     /locus_tag="VEA63_15695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD85510.1"
                     /translation="MSEETEKAGRDAPAEPRIKPAHPESSPYLVLSARGLTKSFLSGD
                     RRIEVLRGVDLTVGAAESVSIRGESGSG"
     gene            complement(208..>1279)
                     /locus_tag="VEA63_15700"
     CDS             complement(208..>1279)
                     /locus_tag="VEA63_15700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007360880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HYD85511.1"
                     /translation="VDTQGDVQVKADGFVQNPAEAMARIENVPGVAAATPYAEGFVML
                     MAGGRPAFPMIQGIDVDRVGRVVPLDRYVRLGSLDALDDDSIILSANLAVTIGARMGD
                     KVEVYSPLLLERLKQDEVFLPRELTVVGIFEVGHQQLDSSLVLVTLRLMQDLYGLRGG
                     IHGINVKLAPGIDADEGAARINAVLPPDAHLEAKSWMDANQDFLFILSLEKNMIFFLL
                     TFIIIVSAFSVTSSLLISVVRKTREIGLLGALGGKSRHVAACFCFQGLFIGVFGTAFG
                     LGLGMTALYFRNGIIRTFTELTSSQEVLTRFYQFSQLPAHTSGSDVMTIVVCAIVIST
                     LAGLLPAWRAARLKPVEALRSE"
BASE COUNT          288 a          474 c          303 g          214 t
ORIGIN      
        1 cccgagcccg attcgccgcg aatcgaaacg ctctcggccg cgcccaccgt gagatcgacg
       61 ccgcgcaaca cctcgatccg ccgatcgccg ctcaaaaaac tcttcgtcag cccgcgcgca
      121 ctcaaaacca ggtagggcga actctccggg tgagccggtt tgatgcgcgg ctcggcgggc
      181 gcgtctcgcc ctgccttctc cgtttcctca ctcactgcgc aaggcctcca ccggtttcaa
      241 ccgcgccgcc cgccacgccg gcagcaaacc cgccagcgtc gaaatcacga tcgcacacac
      301 cacgatcgtc atcacatcgc ttcccgatgt atgcgccggc aactgactaa attgataaaa
      361 ccgagtcagc acttcctggc tgctcgtgag ctccgtgaac gtccggatga ttccatttct
      421 aaaatacaac gccgtcatcc ccagccccag gccgaaagcc gtcccgaaca cgccgatgaa
      481 caatccctga aaacaaaaac acgccgcgac gtgacgcgac ttcccgccta gcgcgccgag
      541 cagcccgatc tcgcgcgtct ttcgcaccac ggaaatgagc agcgaactcg tcacggaaaa
      601 tgccgacacg atgatgataa aggtcagcag gaaaaaaatc atgttcttct ccaacgagag
      661 aatgaacagg aaatcctggt tcgcatccat ccacgacttc gcttcgagat gcgcatccgg
      721 cggcagcacc gcattgatcc gcgccgcgcc ttcatccgcg tcgattcccg gcgcgagttt
      781 cacgttaatc ccgtgaatcc cgccgcgcag cccgtaaaga tcctgcatca accgcagcgt
      841 cactaacacg agcgaactat cgagttgctg atgccccacc tcgaaaatcc ccaccaccgt
      901 caactcgcgc ggtaaaaaca cctcgtcctg cttcaaccgc tcgagcagca gcggcgaata
      961 cacttccacc ttgtcgccca tccgcgcgcc aatcgtcacc gcgagattcg cgctcaaaat
     1021 gatcgagtcg tcatccagcg catcgagcga accgagtcgc acgtaacgat cgagcggcac
     1081 cacgcgaccg acacggtcga cgtcgatccc ttgaatcatc ggaaacgccg gccggccacc
     1141 cgccatcagc atcacgaaac cttccgcgta aggcgtcgcc gccgcgacgc ccggcacgtt
     1201 ttcgattcgc gccatcgcct ccgcgggatt ctgcacaaac ccatccgctt tcacctgcac
     1261 gtcgccctgc gtatccacg
//
LOCUS       DASPLT010001540         1418 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_162260, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001540 DASPLT010000000
VERSION     DASPLT010001540.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1418)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1418)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1418
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_162260"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..530)
                     /locus_tag="VEA63_15705"
     CDS             complement(<1..530)
                     /locus_tag="VEA63_15705"
                     /inference="COORDINATES: protein motif:HMM:TIGR03187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DGQHR domain-containing protein"
                     /protein_id="HYD85512.1"
                     /translation="MIATQIRQKDALFYFASYAPETLLSHVRFISRFYDEEGGGIRPE
                     EVPAEDDVAQFISRVERSDQAFQRELSRAKVGAIRNFYETAISQPPIPGTVLLFTPER
                     LRFDPWEDGSGIGRLQTPQEKFLIIDGQHRLAALQFYLRTHPEEAKNIQVPCVIFDGR
                     SEDFATEMFVIINSTPT"
     gene            886..>1418
                     /locus_tag="VEA63_15710"
     CDS             886..>1418
                     /locus_tag="VEA63_15710"
                     /inference="COORDINATES: protein motif:HMM:NF037959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HYD85513.1"
                     /translation="MALFAFYLVVVTLGVLSMGFQLLASRLLNPHFGSSIIVWAWLIS
                     TFLAAFSIGSMIGGSISNASPGRRDRWRTIAAVAGVATLAFTAFFDRAECAPTEVSFT
                     DMSTGLLVSCLGLFFLPVTALSTFGPQCVQYLATRGTAPGKASGLIYGISTLGNIAGV
                     MLTAFVFIPNFRVSTLL"
BASE COUNT          218 a          389 c          520 g          291 t
ORIGIN      
        1 gtcggcgtgg agttgatgat gacgaacatc tcggtggcga agtcctcgct gcgtccgtcg
       61 aagatcacgc atggcacctg gatgttcttc gcttcctcgg ggtgcgtgcg gagataaaat
      121 tgcagcgcgg cgaggcggtg ctggccgtcg atgatgagga atttctcctg cggtgtttgg
      181 agccgtccga tgccgctgcc gtcttcccac gggtcgaagc ggagccgttc cggcgtgaag
      241 agaagaacgg tgccgggaat cggtggctga gaaatggcgg tttcgtagaa gttgcggatc
      301 gcgccgacct tcgcgcgcga gagctcgcgt tgaaacgcct gatcgctgcg ttcgacgcgc
      361 gagatgaact gcgccacgtc gtcttcggcc gggacttcct cgggtcgaat gccgccgcct
      421 tcctcgtcgt agaagcggct gataaagcga acgtgggaga gcagcgtttc cggcgcgtaa
      481 gacgcgaagt agaacaaggc gtccttctgg cgaatttgag tggcgatcat gggaggagcg
      541 tggcgttgaa cagaacggct gaggtgaacg gccgtcgaag cggcggcctt gcttggaatt
      601 gtgaagcgat tgcgggcgca cgacaagtgg ggaagtggag aagttggcga aacgtggcgc
      661 ggccgcgcgg ggtgcgactg ggagggtcgc taccgtggcg gtgtagcggc aggcctccgt
      721 gcctgcctgc ttggtggctg gttccgaact gccggcgggg cgttcgttgc cggagtcgcg
      781 cgcgagcgcg cctaccgggc ggagcaggca ggcacggagg cctgccgcta cgctgacgga
      841 atttcgaggt gacacgcgcg gggggagcac gcacgttgcg cgctcgtggc gcttttcgca
      901 ttctatctgg tggtcgtgac gctcggcgtg ttgagcatgg gatttcaact gctcgcgtcg
      961 cgcctgctca atccgcactt cgggtcgtcg attatcgtgt gggcgtggct catctcgacg
     1021 ttcctcgcgg cgttcagcat cggctcgatg atcggcggaa gcatcagtaa cgcgtcgccg
     1081 ggacgccggg accgatggcg gacgatcgca gcggtggccg gcgtggcgac gctggcattc
     1141 acggcgtttt tcgatcgcgc cgagtgtgcg ccgaccgagg tgagtttcac ggatatgagc
     1201 acggggttgc tcgtctcgtg cctcggtctg ttttttctgc cggtcacggc gttgtcgacc
     1261 ttcgggccgc agtgtgtgca atatctcgcg acgcgcggca cggcgccggg caaggcgtcg
     1321 ggcctgatct acggcatcag cacgctcggc aacatcgcgg gcgtgatgct gacggcgttc
     1381 gtgttcattc caaacttccg cgtgtccacg ctcttgtt
//
LOCUS       DASPLT010001541         2167 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_264538, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001541 DASPLT010000000
VERSION     DASPLT010001541.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 2167)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2167)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2167
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_264538"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1298)
                     /locus_tag="VEA63_15715"
     CDS             complement(<1..1298)
                     /locus_tag="VEA63_15715"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HYD85514.1"
                     /translation="MASMLDDVPGLMALYRLADLKVAYLNHAANERLNPGGRADTSRL
                     TLWETVGLSSHRRLQAEILPQARVLGRWSGPCDFRDVWGGEFAVTALFKLQRLRGDSY
                     LGVYAHESAATEAVNGTRFTDRQLLHALLDHAPDHIYFKDAASRFLRISRSQSEKFGL
                     ADPAHAIGRTDFDFFTAEHAGRAFADEQQILKTGEPVIDLEEKETRADGRITWVRTTK
                     LPLYDAAGRLIGTFGLSRDITAHKLAEASRKEMEAQVQLAQKMESIGRLAAGVAHEVN
                     TPTQFIADNARFLQDAFARQRAVLARYRELGALAGQHADCAGAVAAAIAEEERAEIDY
                     LSAEIPRSLEQSLQGLARVARIVCSLKEFAHPNSPHLAPADLNHAIETALVVSRYEWK
                     YVAEVETQLDPALPPVPCVADEFNQVVLNMIINAAHAIGD"
     gene            complement(1587..>2167)
                     /locus_tag="VEA63_15720"
     CDS             complement(1587..>2167)
                     /locus_tag="VEA63_15720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD85515.1"
                     /translation="RTMKTFSLKHGLILASLPVLLLAQPGGSDEPIDSTQTARELRQI
                     RGVQELTFGGSGGSDRGFNDSFGGATVGYGVYFNNEWQGAIRQSINYNNPDVGSTRWT
                     GSTRLAADYHFTELGMAMPFVGANFGRIYGDEVHDTWTAGLEVGLKYYVRRKVFVFAM
                     VEYSWFFERGRQLDNNFDEGQFGWSTGIGFNF"
BASE COUNT          418 a          761 c          596 g          392 t
ORIGIN      
        1 tggtcgccga tcgcatgggc cgcgttgatg atcatgttca acacgacttg gttgaactcg
       61 tccgccacac acggcaccgg cggcagcgcc gggtcgagct gcgtctccac ctcggcgacg
      121 tatttccact cgtaacgcga gaccacgagc gccgtttcga tcgcatggtt cagatccgcc
      181 ggggccagat gaggcgagtt cggatgcgca aactccttca gcgagcagac gattcgcgcc
      241 acccgcgcca ggccttggag cgattgctcc aaggagcgtg gaatctccgc actcagataa
      301 tcgatctcgg cacgctcttc ctcggcgatg gctgccgcga ccgccccggc gcaatccgca
      361 tgctgtccgg ccagcgcacc aagctcgcga tagcgagcca gcaccgcccg ctgtcgggca
      421 aacgcatcct gcaaaaaacg cgcgttgtcc gcgatgaact gcgtcggcgt gttcacctcg
      481 tgcgcgacgc cggccgccag gcgaccgatt gactccatct tctgcgccag ctgcacctgc
      541 gcctccattt ccttccgcga cgcctccgcc agcttgtgcg cagtgatgtc gcgcgacagc
      601 ccaaacgtgc cgatgagcct ccccgccgcg tcatagagcg gcaacttcgt cgtgcgcacc
      661 caagtgatcc gaccgtcggc cctcgtctct ttttcctcca ggtcgatgac cggctcgccc
      721 gttttcagaa tctgctgctc gtccgcgaat gccctcccgg catgctcggc cgtaaagaag
      781 tcgaagtccg tcctcccaat cgcatgcgcc ggatcggcca gtccaaactt ttccgactgc
      841 gaacggctga tccgcaggaa gcggctcgcc gcatccttga aatagatgtg atcgggcgcg
      901 tgatcgagca gcgcgtgcaa aagctgccgg tcggtgaacc gcgtgccgtt gacggcctcg
      961 gtcgcagcgc tttcatgcgc atagaccccg agataggaat ctccgcgcag ccgctgcagc
     1021 ttaaacaacg ccgtgacggc aaactcgccg ccccacacgt cgcgaaaatc acatgggcct
     1081 gaccatcggc cgagcacccg ggcttggggc aggatctcgg cttggagccg gcggtgcgac
     1141 gacaagccta cagtctccca caacgtcagc cggctggtat ccgcccgccc gccgggattc
     1201 agccgctcgt tcgccgcatg gttcagataa gcgaccttca aatccgccag ccggtagagc
     1261 gccatcaggc ccggcacatc gtccaacatc gaagccatca actgctttcg ctctaaatcc
     1321 ggtaaaaacg gacacgtggt tcccagcggc tgcggctcag gcgcggtcgc taataaattt
     1381 acagtctcag gcgtggcaca atcttcgtgc cgtccggcgc tcgaaggcat cgctctccta
     1441 tcggcacatc tgcggcaaac tgggctcatt tcacctgcca ccgagcgcgc cgctcggcca
     1501 cttggccgcg cgatcttcgg tgcggcacca gcgcaaatca aagcccggcg acaccaggcg
     1561 ccaatcgcgg tgcgtgcggc aaccgttcag aaattaaacc caatgcccgt gctccagccg
     1621 aactggcctt cgtcgaaatt attgtcgagc tgacgcccgc gttcgaagaa ccagctatac
     1681 tccaccatcg cgaacacgaa caccttccgc cgcacgtagt atttcaaacc gacttcgagc
     1741 cccgccgtcc acgtgtcgtg cacctcgtcg ccgtagattc gcccgaagtt cgccccgacg
     1801 aaaggcatcg ccatcccgag ctccgtgaaa tggtaatccg ccgccagccg ggtcgagccc
     1861 gtccagcgcg tcgacccgac atccgggtta ttgtagttga tcgactgccg aatcgcgccc
     1921 tgccactcgt tgttaaaata aacgccatac cccacggtcg cgccgccgaa cgagtcgttg
     1981 aacccgcgat cgctgccacc gctgccgcca aacgtcagct cctgcacgcc tcgaatctga
     2041 cgcaactcgc gcgccgtttg cgtggagtcg atcggctcgt cggacccgcc cggctgcgcc
     2101 aaaagcaaaa cgggaagact cgcgagaata agaccgtgct ttagagaaaa cgttttcatg
     2161 gttcttt
//
LOCUS       DASPLT010001542         1772 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_162299, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001542 DASPLT010000000
VERSION     DASPLT010001542.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1772)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1772)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1772
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_162299"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            99..1082
                     /locus_tag="VEA63_15725"
     CDS             99..1082
                     /locus_tag="VEA63_15725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD85516.1"
                     /translation="MSLKSASGKAYGQDMRVVPEQEHVTHARLVPAMPAILERLRVHR
                     ESVDREAEQCRDADARNAASDKIGPPLKTYPIGFCRHIRDLVWARAIADPAFRKLIGE
                     GVLVRRVFIFLKGQYFQNAVQLGNLYVDVANDTVWVDKPKLEWMPVADVPYENVERWA
                     TFADVASRYLKVELYPNFLFPGAFPVSPFFAIRRNGRLDLLFAQDALFLKDLGEGMPR
                     IQALLADEALMSRRLPAVYEELLWKKFAGGAPAGFPLTFAPSGMEAIRGGIVREFVEL
                     ANQPPEPGLPAVDGYLRKVMQATRTLSEMDLVPSAAELARLRADGAIPAAA"
     gene            1130..>1772
                     /locus_tag="VEA63_15730"
     CDS             1130..>1772
                     /locus_tag="VEA63_15730"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HYD85517.1"
                     /translation="MSSENRRGVGDTIRWGIIGCGDVTEVKSGPGFQKANDSRLVAVM
                     RRSAGLAEDYARRHGVPRWYDDARALVNDPEVDAVYVATPPGAHFEGVQLAAAAGKPC
                     YVEKPMARNVAECDAMIAACAAAKQTLFVAYYRRALPRFLKVRELVEGGAVGRLTGVN
                     YRMATTTPANADPAKGAWRVSAAESGGGLFLDVGSHVLDFLDFCVGPLEDVHGC"
BASE COUNT          295 a          422 c          691 g          364 t
ORIGIN      
        1 atgtgtttta aaaatcgagt ggaagtatgc cgcgaggtgt tcgcgacgag gagcgttcga
       61 cagctgaatg gcggtcggca gagcggagcg attgcgtcgt gtcgttgaag agcgcgagtg
      121 gcaaggccta cgggcaggat atgcgcgtcg ttcccgaaca ggaacacgtg acgcatgcgc
      181 ggctggtgcc ggcgatgccc gcgattctcg agcggctgcg cgtgcatcgc gagagcgttg
      241 atcgcgaagc cgagcagtgc cgcgatgcgg acgcacgcaa cgccgcgagt gacaagatcg
      301 gcccgccgtt gaagacgtat ccaatcggtt tttgccggca catccgcgac ttggtgtggg
      361 ctcgcgcgat cgcggatccg gcgtttcgga agctgatcgg cgaaggtgtg ctggtgcgcc
      421 gtgtgttcat ttttttgaag gggcagtatt ttcagaacgc cgtgcagttg gggaatctgt
      481 atgtggacgt ggcgaacgac acggtatggg tcgacaaacc gaagctggaa tggatgccgg
      541 tcgcggacgt gccttatgaa aacgtcgagc gatgggcgac gtttgcggac gtcgcgagtc
      601 gctatctgaa ggtggagttg tatccgaatt ttctttttcc cggcgcgttt ccggtgtcgc
      661 cgtttttcgc gattcggaga aatgggcgcc tcgatttgct cttcgcgcag gatgcgctgt
      721 ttctcaagga cctcggcgaa ggcatgccgc ggattcaggc gctgcttgcg gatgaagcgt
      781 tgatgtcgcg gcgactgccg gcggtttacg aggagctgtt gtggaagaaa tttgcgggcg
      841 gagcaccggc gggttttccg ctgacgttcg ctccgagcgg aatggaagcg attcggggag
      901 ggattgtccg ggaatttgtg gagctcgcaa atcagccgcc ggaaccgggg ctgccggcgg
      961 tcgacggcta tttgcgcaag gtgatgcagg cgacgcgaac gttgagcgaa atggatctgg
     1021 tgccgagtgc ggcggagttg gcgcggttgc gcgcggacgg agcgatcccc gccgcggcgt
     1081 gacgcttcgc gggcttgcgg tggttcgagc gcgcgcgttc gctcgcgcga tgtcttccga
     1141 aaatcggcga ggggtgggcg atacgattcg ctggggaatt atcgggtgcg gcgatgtcac
     1201 ggaagtgaaa agcgggccgg gatttcagaa ggcgaatgac tcgcgattgg tcgcggtgat
     1261 gcggcggtcg gcggggctgg cggaagatta tgcgaggcgt catggcgtgc cgcggtggta
     1321 tgacgatgcg cgggcgttgg tgaacgatcc ggaggtcgat gcggtttacg tggcgacgcc
     1381 gccgggcgcg catttcgaag gcgtgcaact cgcggcggcg gcggggaagc cgtgctatgt
     1441 cgagaagccg atggcgcgaa atgtggcgga gtgcgatgcg atgatcgcgg cgtgcgcggc
     1501 ggcgaagcag acgttgttcg ttgcgtatta tcggcgggcg ttgccgcgat ttttaaaggt
     1561 gcgggagttg gttgaaggtg gcgcggtcgg gcggttaacg ggcgtgaatt atcggatggc
     1621 aacgacgacg ccggccaacg cggatccggc gaagggcgcg tggcgggtca gcgcggcgga
     1681 atcgggcggg ggattgtttc tcgatgtcgg ttcgcatgtg ctcgattttc tcgatttctg
     1741 cgtggggccg ctcgaggacg tgcacggctg cg
//
LOCUS       DASPLT010001543         1077 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_183268, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001543 DASPLT010000000
VERSION     DASPLT010001543.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1077)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1077)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1077
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_183268"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..991
                     /locus_tag="VEA63_15735"
     CDS             <1..991
                     /locus_tag="VEA63_15735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HYD85518.1"
                     /translation="KAADTFFSTHVGINKMLVVDAADRLRGLVTSSDVEKITSEAKSR
                     RKPARDSHFRLVVGAALSPVRKPDGSLDREKILSHVGHLVEEHVDAIAVSTAHGHTAG
                     VGEVVKLVRSAYPQLTIIAGNVTSASGVSYLAECGANAIKIGQGPGSICTTRMVAGVG
                     IPQLTALYVAGSVAKKHGVKLIADGGITKSGDIVKALTLSDAVILGGLLAGCREAPGE
                     IMEISGKVYKQYRGMGSLSAMKAGSAARYGHDKNDRTRKVAAEGIEALKEVSGSVDDV
                     LGNLIGGVQSGMGYLGAANLPELRARARYVRISPAGQKEAAPHDVIEVSTRKN"
BASE COUNT          215 a          400 c          288 g          174 t
ORIGIN      
        1 caaggccgcc gacacgtttt tctccaccca cgtcggcatc aataaaatgc tcgtggtcga
       61 cgccgccgat cgcctgcgcg gcctcgtcac cagttccgac gtcgagaaga tcaccagcga
      121 agccaaatcc cgccgcaaac ccgcgcgcga ttcccacttc cgcctcgtcg tcggtgccgc
      181 gctctcaccc gtgcgcaaac ccgacggctc gctcgatcgc gaaaaaatcc tctcgcacgt
      241 cggccatctc gtcgaggaac acgtcgacgc catcgccgtt tccaccgcgc acggtcacac
      301 cgccggcgtc ggcgaagtcg tgaaactcgt ccgcagcgcc taccctcaac tcaccatcat
      361 cgccggcaac gtcacgagcg cttcaggcgt ttcttacctc gccgaatgcg gcgccaacgc
      421 catcaagatc ggccagggcc ccggctcgat ttgcacgacg cgcatggtcg ccggcgtcgg
      481 tatcccgcaa ctcaccgcgc tctatgtcgc cggctccgtc gcgaagaaac acggcgtgaa
      541 actcatcgcc gacggcggca tcacgaagtc cggtgacatc gtgaaagccc tcacgctctc
      601 cgacgccgtc atcctcggcg gcttgctcgc cggctgccgc gaagcgcccg gcgagatcat
      661 ggagatcagc ggaaaagttt ataaacagta tcgcggcatg ggctcgctct ccgcgatgaa
      721 agccggcagc gccgctcgtt atggccacga taaaaacgac cgcacccgca aagtcgccgc
      781 cgagggcatc gaagcgttga aagaagtctc tggttccgtc gacgacgtgc tcggcaacct
      841 gatcggcggc gtgcaaagcg gcatgggcta tctcggcgcg gccaatctcc ccgagctccg
      901 cgcccgcgcc cgctacgtcc gcatcagccc cgccggccaa aaagaagccg ccccccacga
      961 cgtgatcgaa gtctccacac ggaaaaactg aagcgcctct cagctcgcct gccgctcggc
     1021 gatccgatcc gtttgtcata taatagatga caaacgtccg acacgctcgc ccgttct
//
LOCUS       DASPLT010001544         1370 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_99661, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001544 DASPLT010000000
VERSION     DASPLT010001544.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1370)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1370)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1370
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_99661"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..319
                     /locus_tag="VEA63_15740"
     CDS             <1..319
                     /locus_tag="VEA63_15740"
                     /inference="COORDINATES: protein motif:HMM:NF024189.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD85519.1"
                     /translation="LANSAARTATPSPITVTSAPPNPVPEVRTSAPTPDSTWVPGSYT
                     WQNNQWRWTAGRWATPPSPSATWVPGSYNASTRQWTEGHWTTSATNRANLQNPSDTQS
                     PQR"
     gene            complement(516..701)
                     /locus_tag="VEA63_15745"
     CDS             complement(516..701)
                     /locus_tag="VEA63_15745"
                     /inference="COORDINATES: protein motif:HMM:NF019057.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HYD85520.1"
                     /translation="MRFEFWKSTIDRQWYWHLKDERNEKLAQGEAYPTKEACLRAIEA
                     VRKCATATVQDLSLLNT"
     gene            complement(1057..>1370)
                     /locus_tag="VEA63_15750"
     CDS             complement(1057..>1370)
                     /locus_tag="VEA63_15750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD85521.1"
                     /translation="AAARWRWRPAFLGEWERLAGRAVRRRIMENATKRNRKARRQGTV
                     RAPLEKAHTQRSTPESERTVRAGKLVGADKPNARRKTTVTKSKAVRQARGAARKTSSS
                     R"
BASE COUNT          247 a          499 c          369 g          255 t
ORIGIN      
        1 cctcgccaac tccgccgcgc gcaccgcgac cccgtcgccg atcacagtca cgtctgcgcc
       61 accaaacccg gttcccgagg tgcgcacctc cgcgcccacg cccgactcga cgtgggtccc
      121 cggcagttac acgtggcaaa acaatcaatg gcgctggacc gcaggtcgct gggccacacc
      181 gcccagccca agcgcgacgt gggttcccgg ctcttacaac gcctcgacac ggcaatggac
      241 ggaaggccac tggacaacct ccgcgacgaa ccgcgcgaac cttcaaaacc cgagcgatac
      301 acaatcgcca caacgctagc tcgaaccctc tcttcgtccg ccgacaacct ccagcgcgct
      361 acatcgccgc aagcgctgcc gccaacgcca gtgtgagtgt cccacaagaa acgtgaataa
      421 atcgagggcg cggcaacgct ggtagggacg cctctccgag gcgtccgcgc agactgaaat
      481 gcggcgaatt tctaaagtaa tttctgggca ccatactagg tgttgagcag cgacaaatcc
      541 tgcacggtcg cggtcgcgca ttttcgcacc gcttcgatcg ctcgcaggca cgcttccttg
      601 gtcggatacg cctcgccctg cgccagcttc tcattgcgct cgtccttgag gtgccaatac
      661 cactggcggt cgatcgtaga tttccaaaat tcaaatctca cggggaaaag gggcgctgtt
      721 cgccgcgggc ccgaacgcca gcctgggaac gtcccacata acagccccgt tttctccgca
      781 aggaaacggc ctcgactacg taaatttacg cggtgcacga ctgcgccggc ggttcgcctt
      841 gttcgccttg ggattcgcca acgtcgctcc ttccccgacg tcgctccttc cccacctccg
      901 gcgtcaattc gccaccgttg tattttgctt tccctttcga cgcgatcact ccccccgcat
      961 ccggcgccga ccactcacca tccaaagctg gcgtgtttca gtcgaccggt cggtcgaagt
     1021 agaagtttcg ccgaccgctc tcgaacgaca ccgtgctcag cgcgacgaag aagtcttgcg
     1081 cgccgcgcca cgggcctgcc gcacggcttt gcttttcgtc accgtggttt tgcggcgagc
     1141 gttcggcttg tcggcgccga caagttttcc cgcgcgcacg gtgcgctcgc tctccggcgt
     1201 gctccgctgc gtgtgcgctt tttccaaggg agcgcgaacg gtgccctgac gacgcgcctt
     1261 ccgatttcgt tttgtcgcgt tttccatgat acgccgacgg accgcccgac cggccagccg
     1321 ttcccattca cccaaaaaag caggccgcca gcgccagcgc gcggcggcct
//
LOCUS       DASPLT010001545         1376 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_305941, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001545 DASPLT010000000
VERSION     DASPLT010001545.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1376)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1376)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1376
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_305941"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..815)
                     /locus_tag="VEA63_15755"
     CDS             complement(<1..815)
                     /locus_tag="VEA63_15755"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogen peroxide-inducible genes activator"
                     /protein_id="HYD85522.1"
                     /translation="MARARNFSRAAEHCRVAQPSLSQQIMKLEGELGEKLFERTKRTV
                     SLTPAGEVFRLHAERVLDEVELAGESVRESRGVLRGRVALGALPTVAPYYLPTRLRAF
                     SAAYPQVEVVVHEDTTEQLARAVLTKEVDVALVSLPVERTGLVAEEFFDEALLVALPS
                     RHRLAEKRRLTLDDLEEEPFILMKEGHCLAGQALQFCRLNGFAPRVSFRSAQIETVLA
                     FVASGWGVSIVPAMAKVTDSRRVRYRALAGMTRSIGVIYRGARPLNRAARALV"
     gene            980..>1376
                     /locus_tag="VEA63_15760"
     CDS             980..>1376
                     /locus_tag="VEA63_15760"
                     /EC_number="1.11.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009513726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase"
                     /protein_id="HYD85523.1"
                     /translation="MSSSTPQLTTASGIPVADNQNTLTAGPRGPVLLQDFWLLEKLAH
                     FNRERIPERVVHAKGSGAYGTLKITHDITKYSKAKLFAKVGNEAEVFLRFSTVAGERG
                     AADAERDVRGFALKVYTEEGNWDLVGNNTP"
BASE COUNT          267 a          541 c          339 g          229 t
ORIGIN      
        1 tccaccaagg cccgcgccgc ccgattcagc ggccgcgctc cacgataaat cacgccgatc
       61 gaccgcgtca ttcccgccaa cgcgcgatag cgcacccgcc tgctatccgt gaccttcgcc
      121 atcgccggca cgatcgacac gccccacccc gacgcgacga acgccagcac cgtttcaatc
      181 tgcgcactcc gaaagctcac ccgcggggca aaaccattca gccggcaaaa ctgcagcgcc
      241 tggcccgcga gacagtgccc ttccttcatc aggataaacg gctcctcttc caaatcatcc
      301 agcgtcagcc ggcgcttttc cgccagccga tgccgcgacg gcaatgcgac cagcagcgcc
      361 tcgtcgaaga actcctcagc cacgaggccc gtccgctcca ccggcaagct cacgagcgcg
      421 acgtccacct ccttcgtcag caccgcacgc gcgagttgct cggtggtgtc ctcgtgcacc
      481 acgacttcaa cttgtggata cgccgcggaa aacgcccgca accgcgtcgg cagataatac
      541 ggcgccaccg tcggcagcgc gcccaatgcc acccgccccc gcaagacgcc gcgcgattcc
      601 cgcacgctct cacccgccag ttcaacttcg tccaggacgc gctccgcatg cagccgaaac
      661 acctcgcccg ctggagtgag cgacaccgtc cgtttcgtcc gctcgaatag tttttcaccg
      721 agttcgccct ccagcttcat gatctgctgg ctgagcgacg gctgcgccac ccggcagtgc
      781 tccgccgctc ggctgaagtt ccgcgcccgc gccacggcca cgaaataacg caattggtga
      841 agctccatag tcggttccta taactgaaat tggaacttag tctttcccgt ttttcacacg
      901 agtcgataat ctccgcccgt cttcacgcgt ccgggcacca cggcccgcgc gcagcttctc
      961 ttttcactcc cgcattttca tgtcctccag cacgcctcaa ctcaccaccg cctccggcat
     1021 cccggtcgcc gacaaccaga acacgctcac cgccggtccg cgcggtcccg tcctcctcca
     1081 ggatttctgg ctcctcgaaa aactagcgca cttcaaccgc gagcgcatcc ccgaacgcgt
     1141 cgtccacgcc aaaggctccg gcgcctacgg gacgctcaag atcacccacg acatcaccaa
     1201 gtattcgaaa gcgaaactct tcgccaaagt cggcaacgaa gcggaggtct tccttcgctt
     1261 ctccaccgtc gccggcgaac gtggcgccgc cgatgccgag cgcgacgtcc gcggttttgc
     1321 cctgaaggtc tacaccgaag agggcaactg ggacctcgtc ggcaacaaca cgccgg
//
LOCUS       DASPLT010001546         1173 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_19092, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001546 DASPLT010000000
VERSION     DASPLT010001546.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1173)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1173)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1173
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_19092"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1077)
                     /locus_tag="VEA63_15765"
     CDS             complement(<1..1077)
                     /locus_tag="VEA63_15765"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HYD85524.1"
                     /translation="MTQLNLVSAFTTGGIEHALVTGIEAAKESSSPGFGFALGVPTTP
                     LLDPIEEPFSSTGIAWRVRSDSDADSAAVFVLDTIKFNERWEIVAGIRRDRFDVDYRA
                     DRFNDDGTFNRAERIQRTDIETSYRAALVYKPVATGTVYVGWGTSFNPSAEGLSFINS
                     GRNLTIGDSRLDPENNRSLEAGVKWELFRSALYVDAAVFQIEKNNARVPDPATPGFNI
                     LAGKQTVNGFAVNASGRINVFWSLNAGYTYLDSEQERTTQLNVATGTPLANVPKNSYS
                     AWLTWTPTMDLQIGAGGRYVDARLATITQPVKAVGDYWAFDAMASYQLNDNLRVKLNL
                     TNITDEYYFDQLHPFHVVPGPGFGA"
BASE COUNT          213 a          346 c          374 g          240 t
ORIGIN      
        1 cgcaccgaag ccgggaccag gcacgacatg gaaaggatgc agctggtcga agtaatactc
       61 gtcggtgata ttcgtcaggt tcagcttcac gcgcaagttg tcattcagct gatagctcgc
      121 cattgcatcg aaagcccagt agtcaccgac ggcttttact ggttgcgtga tcgtcgcgag
      181 acgggcgtcg acgtagcggc cacccgcgcc gatttgcagg tccatcgtcg gcgtccacgt
      241 gagccatgcg gagtagctgt ttttcggcac gttcgcgagc ggcgtgccgg tcgccacgtt
      301 cagctgcgtc gtgcgttcct gttcgctgtc gagataggtg taaccggcat tcagcgacca
      361 gaagacattg atgcggccag aagcattgac ggcgaaacca ttcaccgtct gcttgccggc
      421 gagaatgttg aagcccggcg tcgcgggatc cggcacgcgc gcattgttct tctcgatctg
      481 gaatacggcg gcgtcgacat acagcgcgct gcgaaacagc tcccacttca cgccggcttc
      541 gaggctgcgg ttgttttccg gatcgaggcg cgaatcaccg atcgtcaggt tgcggccgct
      601 gttgatgaac gacaggcctt cagccgatgg attgaacgac gtgccccagc caacatacac
      661 cgtgccggtt gcgacgggct tgtataccag cgccgcgcga tagctcgttt cgatatccgt
      721 acgctggata cgctcggcgc gattgaacgt gccgtcgtca ttgaagcggt cggcgcggta
      781 atccacgtcg aagcgatcgc ggcggatacc ggcgacgatt tcccagcgct cgttgaactt
      841 gatcgtgtcg agcacgaaca cggcggcgct gtcggcatcg ctgtcggagc gcacgcgcca
      901 cgcgatgccg gtgctgctga acggttcttc gatcggatcg agcaacggcg tcgttggtac
      961 gcctagcgcg aagccgaaac cgggcgacga actctccttc gccgcttcga tgccggtgac
     1021 gagcgcatgt tcgattccac cggtagtgaa cgcgctgacg aggttcagct gcgtcatgaa
     1081 cgtgcgttcg gtgctttcac cgcgaaatat gttgcgattc acgcgcaccg tgtcgagtgg
     1141 cgtgcctgcc ggcggattgc cggcgagcag cgg
//
LOCUS       DASPLT010001547         1243 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_79438, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001547 DASPLT010000000
VERSION     DASPLT010001547.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1243)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1243)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1243
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_79438"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            29..>1243
                     /locus_tag="VEA63_15770"
     CDS             29..>1243
                     /locus_tag="VEA63_15770"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HYD85525.1"
                     /translation="MSNRLGIGGTVKIETQAGVQVRPIALARGYMSSSEPIAHFGLGS
                     VERITRATVSWPSGHVQVFTDLEANRRITVHEPDEPVATTKTAKAHTQFVEVGKFAGL
                     SFVAEEDSFDESALQPLLPRHVNRCGPAIALGDVNGDELDDVVLGGTTRTPPRILVAD
                     GQGYFRETTAKSDLHHPAINDGPALLFDADGDGDNDLLLTSGGVSAPRLSPNYQPRLF
                     LNDAEGRFGPAAEITLPVYTWPVGAASAADFDRDGDLDLFLGGRVEPGRYPLPPGSAI
                     WRNLGDRFEDVTEILAPQLRRCGMVTSALWTDVDNDGWLDLLLACEWGNVRVFANAAG
                     ERLVDRTAALGFSAAGTGFWSSLAASDFNGDGQPDYIAGNLGHNTPYRASGDKPLLLL
                     RGLFEEGGDELTL"
BASE COUNT          220 a          390 c          392 g          241 t
ORIGIN      
        1 tcgggtggtc ttcgtcttgc gcgggaagat gtcgaaccgc ctcgggatcg gcggcaccgt
       61 gaagatcgag acgcaggctg gcgtgcaggt ccggcccata gcgctagcgc gtggctacat
      121 gtcgagcagc gaaccgatcg ctcatttcgg gcttggctcg gtcgagcgga tcacccgcgc
      181 gaccgtctcg tggccctccg gccatgtgca ggtgttcaca gacctcgagg cgaatcgccg
      241 gatcacagtc catgagccgg acgagccggt cgcaacgacc aagaccgcga aggcgcacac
      301 gcaattcgtc gaggttggga aattcgcggg attatcgttc gtcgccgagg aagacagttt
      361 cgacgagtcc gcgctccagc cactgcttcc tcgtcacgtg aatcgctgcg gcccggcgat
      421 tgcccttggc gacgtgaatg gcgacgaact cgacgatgtt gtgctgggcg gcacaacgcg
      481 cacgccaccg cggatcctcg tggcagatgg acaaggatac tttcgtgaaa ccaccgcgaa
      541 atccgatctg catcatcccg cgatcaacga cggccctgcg ctgctcttcg acgccgacgg
      601 ggatggcgac aacgatcttc tgctcacttc cggtggagta tctgcgccac gtctctcccc
      661 gaactatcag ccgcggctgt ttctcaacga tgccgagggt cgctttgggc cagcagcaga
      721 aatcacgctc ccggtgtata cctggccggt cggcgcggcg agcgccgcgg atttcgatcg
      781 cgacggggat ctcgatcttt tcttgggcgg gcgcgttgaa ccgggccggt atcctctacc
      841 gccaggaagc gcgatatggc gcaacctggg cgatcgcttc gaagacgtca ctgaaatcct
      901 cgcgccacag cttcgccgat gtgggatggt cacatcggcg ttgtggaccg acgtggacaa
      961 cgatggctgg ctcgacctgc tcctggcctg cgaatggggg aacgtgcggg tctttgccaa
     1021 tgcggctggc gaacgcctcg ttgatcgaac cgcggcgctt ggtttttccg cggccggcac
     1081 aggattctgg tcctctctgg ccgccagcga tttcaatggc gacggccaac ctgactacat
     1141 cgcaggcaat ctcggacaca acacgccgta tcgagcttct ggtgacaaac ctctcctgct
     1201 gcttcgcggc ctcttcgagg agggtggcga tgaactcacg ctc
//
LOCUS       DASPLT010001548         2431 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_19111, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001548 DASPLT010000000
VERSION     DASPLT010001548.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 2431)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2431)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2431
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_19111"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..813
                     /gene="ftsY"
                     /locus_tag="VEA63_15775"
     CDS             <1..813
                     /gene="ftsY"
                     /locus_tag="VEA63_15775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007359642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HYD85526.1"
                     /translation="LYTADFGVETTNEILEEIKKAYGKDKELRGQEAAQIGAAVLKRV
                     LEGSEGKLEERAGEVSGHKASPTVIAMIGVNGSGKTTTSAKLAWKLKEDGKSVTLAAC
                     DTFRAAAVEQLKSWGTKLDLEIIASHTGADAAAVAFDAWQAANSRGRDYLIVDTAGRL
                     HTKSNLMEELAKIRRVLQKNDPTAPQHRWLVVDGSLGSNSIEQAKAFHQSFGLTGLVV
                     TKLDGTSRGGAIVGIYRQLKIPIYFLGLGEQAEDLQPFSVDNYVKALFGIEE"
     gene            complement(1268..>2431)
                     /locus_tag="VEA63_15780"
     CDS             complement(1268..>2431)
                     /locus_tag="VEA63_15780"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HYD85527.1"
                     /translation="IFNEARFKFGSARYRHVELEGVDVGTVFRNRGHEARVELLHAPL
                     GVFTGALGWQGGRSDFEAVGDEAFLPPSRTATNALFIFEEGDFKPLAWQLGARLERQT
                     IDLRDSSAIGRDHNAFSTSTGLVWTLDDTWTLGASLAHTERAPNAQELFAHGAHIGTN
                     AFEIGDAALDREKSIAVDLTLRKRLGFVTGSLTVFANRFDGYIFEQPTGLVAVAHDDH
                     FHFVPPDDEEAEHGGLPVYRFVQHDAEFHGAELEAIFHLHHAGQRQLDFIVGADVVRA
                     RNRTQRTDLPRITPARLKTGVAWTNGALSLGGEVQWVEHQHRTAPNETPSAGYALASA
                     YVSYRVTFDRAVFDFFLRGANLGDEEARLHTSFLKEVAPLPGRSVTVGVRTTF"
BASE COUNT          459 a          622 c          903 g          447 t
ORIGIN      
        1 ctctacacgg cggacttcgg cgtggagacg acgaacgaga ttttagagga aatcaaaaaa
       61 gcttacggca aggacaagga gctgcgcgga caggaggcgg cgcagatcgg cgcggcggtg
      121 ttgaagcggg tgctggaggg aagcgagggg aagttggagg agagggcggg cgaggtgtcg
      181 gggcataaag cctctcccac agtgattgcg atgatcgggg tgaatggttc ggggaaaact
      241 acgacgtcgg cgaagctggc gtggaaattg aaagaggacg ggaagtcggt gacgttggcg
      301 gcgtgcgata cgtttcgcgc ggcggcggtc gagcagttga aatcgtgggg gacgaagctg
      361 gatctcgaga tcatcgcaag ccacacgggt gcggacgcgg cggccgtggc gttcgacgcg
      421 tggcaggcgg cgaactcgcg cgggcgcgat tacctgatcg tggacaccgc ggggcgcttg
      481 cacacgaaga gtaatctgat ggaggagctg gcgaagatcc ggcgggtgtt gcagaagaac
      541 gatccgacag cgccgcagca tcgctggctc gtggtcgatg gctcgctcgg cagcaactcg
      601 atcgagcagg cgaaggcatt tcatcagagt ttcgggctga cggggttagt cgtgacgaag
      661 ctcgatggca cgagtcgggg cggtgcgatc gtggggattt accggcagtt gaagattccg
      721 atttattttc tgggactcgg cgagcaggcg gaggatttgc agccgttcag cgtggataat
      781 tatgtgaagg cgttgtttgg gattgaggag tagccgcgag cttcgtccgt gcgcggttga
      841 gggcggggag ggctggattt tttacaggag gaaagcagag ggagcagaga tggcgacgga
      901 cggcggttta aataaagtcg tcgccgccga aggtagcggc aggcctccgt gcctgcctgt
      961 ttgtttggaa agggtaggtg gtgcacctgc gcaccacgtt gcgcgcgagc gccaggcagg
     1021 cagagacgcc tgcggctacg ggtaaacggc tgcagctttt tttgagtatg ctttagtagg
     1081 gtgtcccaga ggtaattttt ggaattcgcc gcgtttccat cggcgcggac gcctcggaga
     1141 ggcgtccctg ccggcgctgc cgcgctcctc gatttactca catttcttgt gggacaccct
     1201 accagatttt ggactgtctc tgctcactct gctgcctcct gtaaaatggc tgggattcgc
     1261 ggcaggatca gaaggttgtg cgcacgccga cggtgacgct gcggccgggg agtggagcga
     1321 cttctttaag gaacgaggta tggaggcgcg cctcctcgtc gccgaggttg gcaccgcgta
     1381 ggaagaaatc gaatacagca cggtcgaacg tcacgcggta gctcacgtag gcgctggcca
     1441 gcgcgtagcc ggcggagggc gtttcgttgg gcgccgtgcg atgctgatgc tccacccatt
     1501 gcacctcgcc gccaagcgag agagcgccgt tggtccaagc gacgccggtt ttcaggcggg
     1561 ccggcgtgat acgcggcagg tcggtgcgct gggtgcggtt gcgcgcgcgg acgacatcgg
     1621 cgccgacgat gaagtcgagt tggcgctggc cggcatgatg caaatggaag atcgcctcga
     1681 gctcggcgcc atggaactcc gcgtcgtgtt ggacaaaccg atacacgggc aggccgccgt
     1741 gctccgcttc ctcgtcgtcg ggcgggacga aatggaaatg atcgtcgtgc gcgacggcga
     1801 ccagtcccgt gggttgttcg aaaatatagc cgtcgaaccg gttggcgaaa acggtgagcg
     1861 agccggtcac gaagccgagg cgcttgcgca atgtgagatc gacggcgatg gatttctcgc
     1921 gatcgagtgc ggcgtcgccg atctcgaagg cgttggtgcc gatatgagcg ccgtgcgcga
     1981 agagttcctg ggcgttcggc gcccgctcgg tgtgcgcgag cgaggcaccg agcgtccaag
     2041 tgtcgtcgag cgtccagacg aggccggttg aagtgctgaa agcattgtga tcgcggccga
     2101 tcgcggagct gtcgcgaagg tcgatcgtct ggcgttcgag gcgcgcgccg agttgccagg
     2161 cgagcggttt gaaatcgcct tcctcgaaaa taaacagcgc gttggtggcg gtgcgcgacg
     2221 gcggcagaaa agcctcgtct ccgacggcct cgaaatcgct gcgaccgcct tgccagccga
     2281 gcgcgccggt gaagacgccg agcggcgcgt gaagcagctc gacgcgggcc tcgtgaccgc
     2341 gattgcggaa cacggtgccg acgtccacgc cttcgagttc gacgtggcga tagcgggcgg
     2401 agccgaactt gaagcgtgct tcgttgaaaa t
//
LOCUS       DASPLT010001549         1337 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_162468, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001549 DASPLT010000000
VERSION     DASPLT010001549.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1337)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1337)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1337
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_162468"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1232)
                     /locus_tag="VEA63_15785"
     CDS             complement(<1..1232)
                     /locus_tag="VEA63_15785"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HYD85528.1"
                     /translation="MTVARLTLMLALFAGSAIHAFAAPVFNVLFLVADDLNCDLGCYG
                     AREMKTPNIDRLAAHGVRFDRAYCQYPLCSPSRASFLTGRRPNATGVLLLPTRKEPVS
                     PHFRQFIPDTVTLPQLFKQHGWFSARVGKMYHYAVPREIGTGGLDDTPSWDLAINPRG
                     RDRDDESRITLMHPGQFGDTLSWLAADGDDTEQTDGVGATEAIALLEKFKREDRRFFL
                     GVGFFRPHTPYVAPKKWFDLYPSDDVALPELSADDTTRTPAPAYASSRKGQDAASDDL
                     RREAIRAYHASTSFVDAQVGRVVDALDRLGLRESTVIVFTSDHGYHLGDHGLWQKYSL
                     FERSARVPLIIAVPNAKSKGAGARGLVEMVDLYPTLAGLCGLPTPNYVEGVDLRPILE
                     EPARTVKAAAFSQIKRGNV"
BASE COUNT          245 a          453 c          408 g          231 t
ORIGIN      
        1 acgttgccgc gcttgatctg gctgaacgcc gccgctttca ccgtccgcgc cggctcctcc
       61 agtataggtc gcaggtccac accctcgacg tagttcggcg tcggcaggcc gcaaagtccc
      121 gcgagcgtcg gataaagatc gaccatctcc acgagaccac gcgccccggc gcccttcgac
      181 tttgcgttcg gcaccgcgat gatcaacggc acgcgcgcgc tgcgctcgaa gaggctgtat
      241 ttctgccaaa gcccgtggtc gccgagatga taaccgtgat cgctggtgaa cacgatgacg
      301 gtgctctccc gcaagccgag ccggtcgagc gcgtcgacca cccggccgac ctgcgcgtcg
      361 acgaacgatg tcgacgcgtg atatgcgcgg atcgcttcgc gccgcaggtc gtcgctcgcc
      421 gcatcctgcc ctttccgcga actcgcataa gcaggcgccg gtgtccgcgt tgtgtcgtcg
      481 gcactcagct cgggcagcgc gacgtcgtcg gacggataca aatcgaacca ctttttcggc
      541 gcgacatagg gcgtgtgcgg tcgaaagaag ccgacgccga gaaagaaccg ccggtcctcg
      601 cgcttgaact tctccaacaa cgcgatcgcc tccgtcgcac cgacgccgtc cgtctgctcc
      661 gtatcgtcac cgtcggccgc gagccagctc aacgtgtcgc cgaactgccc gggatgcatg
      721 agcgtgattc ggctttcgtc gtcgcggtcg cgaccgcgcg gattgatcgc gagatcccac
      781 gagggcgtgt cgtcgagccc gcccgtgccg atttcacgcg gcacggcgta atgatacatt
      841 ttcccgacgc gcgccgaaaa ccatccgtgc tgtttgaaca gttgcggcag cgtgaccgta
      901 tccggaataa actggcgaaa atgcggcgag accggctcct ttctcgtcgg cagcagcagg
      961 acgcccgtcg cgttcggacg acgaccggtg agaaacgacg cgcgactcgg cgaacaaagc
     1021 ggatactggc aatacgcgcg atcgaaacgc acaccgtgcg ccgcgaggcg gtcgatgttc
     1081 ggcgtcttca tctcgcgcgc accgtagcag ccgagatcgc agttcagatc gtcggccacg
     1141 agaaacaaca cgttgaaaac cggcgcagcg aaggcgtgga tcgcgctacc ggcgaacaaa
     1201 gcgagcatca gcgtgagtcg cgcgaccgtc atggcgttga cccggttttg ttcgctgctt
     1261 cagacgcacc ctcggcggcc gccggagtcg tgggttcacc gacgacccct ttgctctgca
     1321 gctcgcccaa ctccact
//
LOCUS       DASPLT010001550         1443 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_284282, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001550 DASPLT010000000
VERSION     DASPLT010001550.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1443)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1443)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1443
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_284282"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1443)
                     /locus_tag="VEA63_15790"
     CDS             complement(<1..>1443)
                     /locus_tag="VEA63_15790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140658 - ATP-dependent chromatin
                     remodeler activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2-related protein"
                     /protein_id="HYD85529.1"
                     /translation="LAENRARLRDHFDAEFTANFEKNTARLRDAEIKAEATELGGEFN
                     LTLAVRAGSASDDALRSAVSSQRGYVEADGRVYLLDSARLRQLDAAQHALAGAPGGGP
                     TTRRTHRVTAARLAEAQEILETLSPDFQPPETWRARSEALRNLSRLAPAPLPPPLEAQ
                     LRPYQRLGAAWLWHLHRHQLGGILADEMGLGKTLQALALLTAVTTNRVPNSEPRTSNS
                     ELRTPNSNLRSPNSDLRTPSLVVCPATLLENWRRESARFAPQLRIFVHHGDRRLTSAA
                     DIAAFDLVVTSYGTLTRDWELFSSFSFTCVIADEAQHIKNRRSQNAAALRSLRARSRF
                     LLTGTPLENSLDDLRSLFEFLLPGYLDKVPSGLRADERAWFDDRLRAKVAPYILRRTK
                     QAVAPELPAKLEQIVWCELAPAQAALYRETQQRTERELFDLEAGGASEASLRFAALTQ
                     LLRLRQICCDPRLIAQSKTQNPDSGTQNPKP"
BASE COUNT          231 a          338 c          618 g          256 t
ORIGIN      
        1 gggtttcggg ttttgggttc cggagtcggg gttttgggtt ttcgattggg cgatgaggcg
       61 ggggtcgcag cagatttggc gaaggcggag gagttgggtg agggcggcaa agcggaggct
      121 ggcttcgctg gcgccgccgg cttcgagatc gaagagctcg cgctccgtgc gttgctgcgt
      181 ctcgcggtag agtgcggctt gtgcgggggc gagctcgcac cagacgattt gctcgagctt
      241 ggccgggagc tcgggggcga cggcttgctt ggtgcgacgg agaatgtagg gcgcgacttt
      301 ggcgcgcaga cggtcgtcga accaggcgcg ttcgtcggcg cggagaccgg acggaacttt
      361 gtcgagataa ccgggaagca ggaactcgaa gagcgaacga agatcgtcga gggagttttc
      421 gagcggcgtg ccggtgagga ggaagcgact gcgggcgcga agcgaacgga gcgcggcggc
      481 gttttgggag cggcggtttt tgatgtgctg ggcttcgtcg gcgatgacgc aggtgaagct
      541 gaacgaggag aacagttccc aatcgcgcgt gagcgtgccg taggacgtga cgacgaggtc
      601 gaacgcggcg atgtcggcgg cggacgtcag gcggcggtcg ccgtgatgga cgaagatgcg
      661 gagttgagga gcgaagcgcg cggattcgcg gcgccagttt tcgaggagcg tggcgggaca
      721 gacgacaagc gacggggttc ggagatcgga gttcggagat cggagattgg agtttggagt
      781 tcgaagttcg gagttcgaag ttcgaggttc ggagttcgga acacggttgg tggtgacggc
      841 ggtgaggagg gcgagggctt ggagggtttt tccgagaccc atttcgtcgg caagaatgcc
      901 gccgagttgg tggcggtgaa gatgccagag ccacgcggcg ccgaggcgtt gatacgggcg
      961 aagctgggct tcgagcggcg gcggcaacgg cgcgggcgcg aggcgcgaga gattgcgcag
     1021 agcttcgctg cgcgcgcgcc aggtttcggg cggttggaaa tcgggcgaaa gcgtttcgag
     1081 aatttcctgc gcttcggcga ggcgggcggc ggtgacgcga tgggtgcggc gcgtggtcgg
     1141 tccgccgccg ggcgcgccgg cgagcgcgtg ttgcgcggcg tcgagctggc ggaggcgggc
     1201 ggagtcgagg aggtaaacgc gaccgtcggc ttccacgtat ccacgttgtg aggacacggc
     1261 ggagcgaagg gcgtcgtcgc tggcggaacc ggcgcggacg gcgagcgtga gattgaactc
     1321 gccgccgagc tcggtggctt cggctttgat ctcggcgtcg cggagccgtg cggtgttttt
     1381 ctcgaagttg gcggtgaact ccgcgtcgaa atgatcgcga aggcgggcgc ggttttccgc
     1441 gag
//
LOCUS       DASPLT010001551         2622 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_264860, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001551 DASPLT010000000
VERSION     DASPLT010001551.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 2622)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2622)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2622
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_264860"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..625)
                     /locus_tag="VEA63_15795"
     CDS             complement(<1..625)
                     /locus_tag="VEA63_15795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="serine acetyltransferase"
                     /protein_id="HYD85530.1"
                     /translation="MRADRMDENLEPITAAILRSYAMEGGINHVDGANLPSKRRIADI
                     CEDLLQVLFPGFHDGESIHSRDLARVTSRRVIDISKRMVDEICKSLRLQTPDCPKELA
                     AVQARHFIGGLPGVRALLATDVEAAFTGDPAAGGRDEVILSYPFIEAIAIQRAAHVLQ
                     ALKVPVIPRMMTEWAHSRTGIDIHPGATIGSHFFIDHGTGVVIGETSV"
     gene            complement(1032..>2622)
                     /locus_tag="VEA63_15800"
     CDS             complement(1032..>2622)
                     /locus_tag="VEA63_15800"
                     /inference="COORDINATES: protein motif:HMM:NF037016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HYD85531.1"
                     /translation="KNKTSRLGYFAYRPQEDYEGEDSFSYTVRNETSGLVFENTVLIT
                     IKPPSPVMLDKLEVGASRERSMDVREVALATRPNTPIAHRLPSHQDFMTPADRAAIVD
                     PKVAYLLDEKAKPQNGTAKLDRATGQLTYAPNPGFIGVDRFTYYTVDENNAHLGVENA
                     VSVNVEPIRTVKHMTADRSRSREVDLVFVINNSPSMAAHQSRIAANLSRFRQLFHTRD
                     LDYRIGVLTTDFVNAEPGRRPEDQPYFKEVRSVQLDKAGRPVLDRRDRPKQITKRVAS
                     NGTLVTLPVMEQPWVTPRTPDSVFAELVKVGTNGDSNRTAFTSVYNFVAGYYNKQHAF
                     LRPDATTIVVFFMDEEETRMAVWKEQKDGPPQAEWIENGKLPELLGHYNASNPEKRQT
                     LDGYINYWVLRPFIIAKGNKRGKLEMHAVVSPNNVSHRRAAELTGGTVLNIESDFSGP
                     LAALGDRIADTVAVALEPVEATATFYQKSLRVLVDGEEVRADPENGYVYDELTHSIRF
                     QGDAKKKAFTAKIDVTYEEHK"
BASE COUNT          458 a          824 c          815 g          525 t
ORIGIN      
        1 tcacgctcgt ctcgccgatc acgacgccag ttccgtgatc gataaagaaa tgtgagccga
       61 tggtcgcgcc aggatgaatg tcgatgccgg tgcggctgtg cgcccattcc gtcatcatcc
      121 gcggaatgac cggcaccttc agcgcctgaa gcacatgcgc cgcccgctgg atcgcaatgg
      181 cctcgatgaa gggataactc aggatcactt catcgcgtcc accagccgcc ggatctccgg
      241 taaacgccgc ctccacatcc gtcgcgagca acgcccgcac gcccggcagt ccgccgatga
      301 aatgccgcgc ctgcaccgcc gccagttctt tcggacaatc cggcgtctgc aatcgcaggc
      361 tcttgcagat ttcgtccacc atccgcttgg aaatgtcgat gactcgccgg ctcgtgaccc
      421 gcgccaaatc ccgactgtgg atcgactccc cgtcgtggaa accgggaaag agcacttgca
      481 gcaggtcctc gcagatatcg gcgatcctgc gcttggacgg caaattagcg ccgtccacgt
      541 gattgatccc accctccatc gcgtaagagc ggaggatggc agcggtaatg ggctcgagat
      601 tttcgtccat tcgatcggca cgcacggtga atgaccgcgc atcggcgtgc gacgtttctt
      661 tgcagattca aggtcgcgtg cagcgatttc ctcgaaccga acggaccgtc gccaatcgat
      721 caagccgcgc cgacgctcgc tcgaacgaag cagcgctcga ctgcgcggcg tgaacaggct
      781 cacgcaagta tgcgccatga tcgcgcaaac attggcgcag ccgggcaacg tcgactgcat
      841 gcacatctcc tgatcgggct cacgggcccg ccagccggca cccacttgct gaagatcggt
      901 ggcagcggca ctgccggagt gacagccaac ttcgacgtgg acgcggctag cctcggtagc
      961 ggacgacctt cccgtcatca cgacgcagca gtgcgcgccg ccattgcggg cttccgtcgc
     1021 gcgcgcacgc gtcatttgtg ctcttcgtag gtgacgtcga tcttggcggt gaacgccttc
     1081 ttcttcgcgt cgccctggaa gcggatgctg tgcgtgagct cgtcgtaaac gtatccgttt
     1141 tccgggtcgg cgcgcacttc ctcaccgtca accagcacgc ggaggctctt ctggtaaaag
     1201 gtcgcggtgg cctcgaccgg ctcgagcgcg acggcgaccg tatcagcaat gcggtcaccg
     1261 agcgcggcaa gcggtccgga gaaatcgctc tcgatgttca gcaccgtgcc gccggtgagc
     1321 tcggccgcgc ggcggtgcga gacgttgttt ggcgacacca ccgcatgcat ctcgagtttt
     1381 ccgcgcttgt tccctttcgc gatgatgaag gggcgcagca cccagtaatt gatgtagccg
     1441 tcgagcgtct ggcgtttctc cggattgctg gcgttgtagt ggccgagcag ctccggaagc
     1501 ttgccgtttt cgatccattc ggcctgaggc gggccgtcct tttgctcctt ccaaacggcc
     1561 atgcgcgtct cctcctcatc catgaagaag accacgatcg tggtggcgtc ggggcgcaga
     1621 aacgcgtgct gcttgttata ataacccgcg acgaaattat acaccgatgt gaacgccgtg
     1681 cggttgctgt cgccattcgt gcccaccttc acgagctcgg caaaaacgct gtccggcgtc
     1741 cgcggcgtca cccacggctg ctccatcacc ggaagcgtca cgagcgtgcc gttgctcgcg
     1801 acgcgcttgg tgatctgctt cggccgatcg cggcggtcga gcaccgggcg gcccgctttg
     1861 tcgagctgca cggagcggac ttccttgaaa tagggttggt cttcggggcg acgtccaggc
     1921 tccgcgttca cgaaatcggt cgtgagcacg ccgatgcggt aatcgagatc gcgcgtgtgg
     1981 aagagctgcc gaaagcgact gagattggct gcgatgcggc tctggtgcgc ggccatcgag
     2041 ggcgaattgt tgatgacgaa cacgaggtcg acttcgcgac tgcgcgagcg atcggccgtc
     2101 atgtgcttca cggtgcgaat cggctcaacg ttgaccgaaa cggcattctc cacgccgagg
     2161 tgcgcgttgt tctcgtcgac cgtgtagtag gtgaagcgat ccacgccgat gaaacccggg
     2221 ttcggcgcat aagtgagctg gcccgtcgcg cggtcgagtt tcgccgtgcc gttctgcggt
     2281 tttgctttct cgtccagcag gtatgcgacc ttcgggtcga cgatcgcagc gcggtcggcc
     2341 ggcgtcatga aatcctggtg gctgggaagc cggtgcgcga tcggcgtgtt cggccgcgtc
     2401 gcgagcgcca cttcgcgaac atccatcgag cgctcgcggc tggcgccgac ttccagcttg
     2461 tcgagcatca caggcgaagg cggtttgatc gtgatcagga cggtgttttc gaacacgagg
     2521 ccggaggttt cattgcgcac cgtgtagctg aaggaatcct cgccctcgta atcctcctgc
     2581 ggtcgatacg cgaaataacc gaggcgcgac gtcttgttct tg
//
LOCUS       DASPLT010001552         1040 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_142460, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001552 DASPLT010000000
VERSION     DASPLT010001552.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1040)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1040)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1040
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_142460"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..208
                     /gene="metX"
                     /locus_tag="VEA63_15805"
     CDS             <1..208
                     /gene="metX"
                     /locus_tag="VEA63_15805"
                     /EC_number="2.3.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374292.1"
                     /note="Catalyzes the conversion of acetyl-CoA and
                     L-homoserine to CoA and O-acetyl-L-homoserine; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="homoserine O-acetyltransferase"
                     /protein_id="HYD85532.1"
                     /translation="GFTSDWLFPPEQNRAITLALLRAGKRASYAELATDLGHDSFLLE
                     SEELYTLVRGFLETASNSAVDFSI"
     gene            305..913
                     /locus_tag="VEA63_15810"
     CDS             305..913
                     /locus_tag="VEA63_15810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine biosynthesis protein MetW"
                     /protein_id="HYD85533.1"
                     /translation="MTKLVEKRTVDMQIIGEWVDPSSRVLDLGCGKGALLDYLVQTKQ
                     VSGVGVDLDFHRITACVARGLSAYQGDMTSFMREFPDRHFDRVICSRTVQELDDPTAV
                     ILEALRVAHAVTVGFVNHGFWKNRVDALIRGRKLRNEVYTTEWFESRPTNPLTIADFE
                     HFCAVKGIRIVRRAHLRGNWHTPCRTFPNLFAGYALYDLAKN"
BASE COUNT          198 a          364 c          266 g          212 t
ORIGIN      
        1 cggcttcacc agcgactggc tgtttcctcc cgagcaaaac cgcgccatca ccctcgccct
       61 gctccgcgcc ggcaaacgcg ccagctacgc cgaactcgcc accgacctcg gtcacgactc
      121 gttcctgctc gaatccgaag aactctacac cctcgtccgc ggtttcctcg aaaccgcctc
      181 caactccgcc gtcgatttct cgatctgaga agtaaccacg aatggacacg aagagacacg
      241 aatccggact gcttgaattc attcgtgtga attcgtgtcc attcgtggtt aacaaaaatt
      301 acccatgacc aagctcgtcg agaaacgcac cgtcgacatg cagatcatcg gggaatgggt
      361 cgaccccagt tcccgcgtgc tcgatctcgg ctgcggcaag ggcgcgctcc tcgattacct
      421 ggtgcagacg aaacaggttt ccggcgtcgg cgtggatttg gatttccatc gcatcaccgc
      481 ctgcgtcgcg cgcggcctct ccgcgtatca aggcgacatg acgtcgttca tgcgcgagtt
      541 tcccgaccgg cacttcgatc gcgtcatctg ctcgcgcacg gtccaggagc tcgacgatcc
      601 caccgccgtc attctcgaag cgctgcgcgt cgcgcacgcc gtgaccgtcg gtttcgtcaa
      661 tcacggcttc tggaaaaacc gcgtcgatgc gctcatccgc ggacgcaaat tgcgcaacga
      721 agtctacacc accgagtggt tcgagagccg tccgacgaat ccgctgacga tcgccgactt
      781 cgaacacttc tgcgccgtga aaggcatccg catcgtccgt cgcgcgcacc tgcgcggcaa
      841 ctggcacacg ccttgccgca cgtttccgaa cctcttcgcc ggctacgcgc tctacgacct
      901 cgcgaaaaac taagccgctc atgcgaagag agctggattc gccctgtttt cattcgggtc
      961 gattcgtgtc cattcgtggt tgagttgaat accgccaccc cgcctcgttt gcatcggctc
     1021 cggacctcgt caccttgccc
//
LOCUS       DASPLT010001553         1377 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_284352, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001553 DASPLT010000000
VERSION     DASPLT010001553.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1377)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1377)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1377
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_284352"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..744
                     /locus_tag="VEA63_15815"
     CDS             <1..744
                     /locus_tag="VEA63_15815"
                     /inference="COORDINATES: protein motif:HMM:NF015251.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD85534.1"
                     /translation="LVNLQLCFPEKSPAEIRALARAHYQSLALGLFETCTGWWTPAQK
                     LPPHTIVGHEHLERAIAGGKGVILLTAHFTTLELCARMMNERFDLSVLYRDPNNPVLA
                     AEMRAQRERHVNIAVHFDDLKGLIRALRAGEAMWYAPDQGKRTKLTEILPFFGIPAIT
                     NTATGKLAGMTGAPVVPYFAKRETDGSYLLTILPPLENFPTADATADAIRVNQLIEQH
                     IRLAPEQYLWVHKRFKARGPGYPEVYKRK"
     gene            826..>1377
                     /locus_tag="VEA63_15820"
     CDS             826..>1377
                     /locus_tag="VEA63_15820"
                     /inference="COORDINATES: protein motif:HMM:NF013257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HYD85535.1"
                     /translation="MSIRGLKKMSYPDPRSLRILVIRYRFIGDTVLAIPFLRNLRRAF
                     PHATIDVLAEPVSGETLAHCPYKNDLLFYAPRAKKKTPHPQSLLACARFLRARRYDRA
                     YILRRSFSSAILPLLAGIPHRVGFATEGRRWLLQRSTPYPDKHEVECFLDILRADRIP
                     VLDTFNENWSDPDVDRRVDATLAS"
BASE COUNT          264 a          540 c          324 g          249 t
ORIGIN      
        1 ctcgtcaacc tccagctctg cttccccgaa aaatctcccg ccgagattcg cgccctcgct
       61 cgcgcacatt accaatcgct cgcgctcggc cttttcgaaa cgtgcaccgg ctggtggacc
      121 cctgcgcaaa aactcccgcc tcacacgatc gtcggtcacg aacacctcga gcgcgccatc
      181 gcgggcggca aaggcgtcat cctgctcacc gcgcatttca ccacgctcga actctgcgcg
      241 cgcatgatga acgagcgttt cgatctcagc gtgctttatc gcgatccgaa caatcccgtc
      301 ctcgccgccg aaatgcgcgc gcagcgcgag cgtcacgtca acatcgccgt tcacttcgac
      361 gatctcaaag gcttgatccg cgcactccgc gccggcgaag cgatgtggta tgcgcccgac
      421 cagggcaaac gcaccaagct caccgaaatc cttccgttct tcggcatccc cgccatcacc
      481 aacaccgcca ccggcaaact cgccggcatg accggtgcgc ccgttgtccc ctatttcgcc
      541 aaacgcgaaa ccgacggttc ctacctcctc acgattctcc cgccgctcga aaactttccc
      601 actgccgacg ccacagccga cgcgattcgc gtgaaccaac tcattgagca acacatccgc
      661 ctcgcgcccg agcaatacct ttgggtgcac aagcgcttca aagcccgcgg cccgggctat
      721 cccgaagtct acaagcggaa ataaaccacg aatggacacc cgtgcacacg aatgcagagg
      781 accggcggcc atcgtgcgcg gcccgtcttt tattcgtgtc ccttcgtgtc cattcgcggt
      841 ttaaagaaaa tgagctaccc cgatccgcgc tccctgcgca ttctggtgat ccgctaccgc
      901 ttcattggcg acaccgtcct cgcgattccg tttctgcgaa acctccgccg cgcgtttcct
      961 cacgccacga tcgacgtcct cgccgaaccc gtctccggtg aaaccctcgc gcactgcccg
     1021 tataaaaacg acctgctctt ctacgccccg cgcgcgaaga aaaaaacgcc gcacccgcaa
     1081 tcgctcctcg cgtgcgcgcg cttcctccgc gcccgccgct acgaccgcgc ctacattctc
     1141 cgccgctcgt tctcgagtgc gatccttccg ctgctcgccg gcattccgca tcgcgtcggc
     1201 tttgccaccg aaggacgccg ctggctcctg caacgcagca cgccctaccc cgacaaacac
     1261 gaagtcgagt gcttcctcga catcctccgc gccgaccgca tccccgtttt ggatacgttc
     1321 aacgaaaact ggagcgaccc cgatgtcgac cgccgcgtcg acgccacgct cgccagc
//
LOCUS       DASPLT010001554         2183 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_183432, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001554 DASPLT010000000
VERSION     DASPLT010001554.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 2183)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2183)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2183
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_183432"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..715
                     /locus_tag="VEA63_15825"
     CDS             <1..715
                     /locus_tag="VEA63_15825"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HYD85536.1"
                     /translation="VLEEARRLGRTIHVNSPPPEPPAYLTRILEWQREHSLGRPISCQ
                     GEMLIAYREKQDGRWLDDLALCPAAPIFRLGVYSLNDLVHVFGPVRAVQVLSSRLFTG
                     RPTADNAQLGLMFENGAIGSVHASFCVDNGQHYANALTLNYERGSIYRNTSPVDYGRA
                     EHSSRLLLVATRPSGELVREHWQSEEASGDYPWQSFHDAVSGARAVETPIDDVVGTTA
                     VLAAMGRAERSGKTELVAF"
     gene            737..1864
                     /locus_tag="VEA63_15830"
     CDS             737..1864
                     /locus_tag="VEA63_15830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HYD85537.1"
                     /translation="MITTPLPRLTLLILALGFLATAVQADYDFGDWPKGSSPEEVGKR
                     VVERFISRDYRLLRSGVVHYVETCTWYGALSFAKLTNDPATTQRVVDRFQPLFGEKAK
                     HIPPPENVDYAVFGALPLEIYIQTKDERYLTLGLSLADAQWTTPGDESRLDAEAREAV
                     AKGLSWHTRFWIDDMYMITMLQTQAYRATGDAKYLNRAAEEAVAYLERLQQPNGLFHH
                     APDVPFFWGRGDGWFAAGMSELLRALPANHPQRARILESYRKMMASLLKHQDADGMWH
                     QLIDDPNSWPETSCTGMFTFAFITGVKHGWLDAATYGPAARKGWLALLNYIEPNGDIR
                     EVCEGTNKKNERQYYLDRLRITGDLHGQAPILWCASAFLGL"
     gene            1886..>2183
                     /locus_tag="VEA63_15835"
     CDS             1886..>2183
                     /locus_tag="VEA63_15835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD85538.1"
                     /translation="MIRNASILLCVALALLTTGRAAAAPAAPVVDLSIEETADLIRVS
                     HAGGELVVERVPWRLRLNGADRAERFNEAAPPALQIAGVWQPVKEVTGVRRNGDA"
BASE COUNT          389 a          618 c          745 g          431 t
ORIGIN      
        1 cgtcctggaa gaggcgcgga ggctgggccg gacgattcat gtgaactcgc cgccgccgga
       61 gcctccggcg tatctcacgc gaattctcga atggcagcgc gagcactcac ttggccggcc
      121 catcagctgc cagggggaga tgctgattgc gtatcgggaa aagcaggacg ggcgctggct
      181 ggacgatctg gcgttgtgtc cggccgcgcc gattttccgg ctgggcgttt actcgttgaa
      241 cgatcttgtg cacgttttcg gtccggttcg tgcggtgcag gtgctgagca gccggctgtt
      301 caccggacgg ccgacggcgg acaatgcgca actcgggctc atgttcgaaa acggcgcgat
      361 cggcagtgtc cacgcgagtt tctgcgtgga caacggtcag cattacgcga atgcgctgac
      421 gttgaactac gagcgcgggt cgatctatcg aaacacgtct ccggtcgact acgggcgggc
      481 ggaacactcg tcgcgcttac tgctggtcgc gacgcgtccc agcggagagt tggtgcgcga
      541 gcactggcag tcggaggaag cttccggcga ttatccgtgg cagagttttc acgatgctgt
      601 ctccggggcg cgcgcggtcg aaacgccgat cgatgacgtt gtcggcacga cggccgtgct
      661 ggccgcgatg ggccgcgccg aacgttccgg aaaaaccgaa ctcgttgctt tctgagtcac
      721 cgcactcgct ctatttatga ttacgacccc gttaccccgc ctgacgttgc tcattcttgc
      781 gctcggtttt cttgcgaccg cggtgcaggc cgattacgac ttcggcgact ggccaaaggg
      841 ctcgtcgccg gaggaggtgg gcaagcgcgt tgtggaacgt ttcatttcgc gcgactatcg
      901 tctgctccgg agtggcgtgg tgcactacgt ggaaacgtgc acgtggtatg gtgcgctttc
      961 gttcgcgaag ttgacgaacg atcccgcgac gacccagcgg gtcgtcgatc ggtttcaacc
     1021 gctgttcggt gaaaaggcga agcacatccc gccgcccgag aacgtggatt acgcggtgtt
     1081 cggcgcgttg ccgctggaga tttacatcca gaccaaggat gaacgttatc tgacgttggg
     1141 tctgagcctg gcggatgcgc agtggacgac gccgggcgac gagagtcggc tcgatgcgga
     1201 ggcccgtgaa gcagtcgcga agggcctgag ctggcacacg cgtttctgga tcgatgacat
     1261 gtatatgatc acgatgctgc aaacgcaggc gtatcgggcg actggagacg cgaagtattt
     1321 gaaccgcgcc gcggaggaag ccgtggctta cctggaaaga ctgcagcagc cgaacggcct
     1381 tttccatcac gcgccggacg tgccgttttt ttggggccgc ggcgacggct ggtttgccgc
     1441 ggggatgtcc gaactgctgc gcgcattgcc agcgaatcat ccgcagcggg ctcgcatcct
     1501 cgagagctac cgaaaaatga tggcgagtct gctcaagcat caggacgcgg acggcatgtg
     1561 gcatcagctc atcgacgatc cgaattcctg gcctgagacg tcgtgcaccg gtatgttcac
     1621 gttcgcgttc atcaccggag tgaaacacgg gtggctggat gcggcgactt acgggccggc
     1681 tgcacgcaaa ggctggctgg cgttgctcaa ctacatcgag ccgaatggcg acattcgcga
     1741 ggtgtgcgaa ggcacgaaca agaaaaacga acgccaatac tacctcgacc ggttgcgcat
     1801 cacgggcgat ctccacgggc aggcgccgat cctgtggtgc gcgtcggctt tcctcgggct
     1861 gtaggtgagg acctgaatat cgatcatgat ccgaaacgcc tcgattctgc tttgcgttgc
     1921 gctcgcgcta ttgacgactg gacgcgccgc cgcagcgccg gccgcgccgg tcgtcgatct
     1981 gtcgatcgag gagacggcgg accttattcg tgttagccac gcgggtgggg aacttgtggt
     2041 cgagcgcgtt ccgtggcggt tgcggttgaa cggtgccgat cgtgcggagc gatttaacga
     2101 ggccgcgccg ccagcgctgc agatcgcggg cgtctggcag ccggtgaagg aagtcaccgg
     2161 tgtgcggcgg aatggcgatg cgg
//
LOCUS       DASPLT010001555         2708 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_58391, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001555 DASPLT010000000
VERSION     DASPLT010001555.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 2708)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2708)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2708
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_58391"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(301..2490)
                     /locus_tag="VEA63_15840"
     CDS             complement(301..2490)
                     /locus_tag="VEA63_15840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012372891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HYD85539.1"
                     /translation="MQNPFWPRIAALLLIGVAVSLSPLHAAADRDWRVYLGDQAASHY
                     STLAQITPENVAQLEVAWTFNAGDASGQTQIQCNPLVIDGVLYGTTPRLKVFALDAAT
                     GRELWRFEPVESNGVNRGLAYWQSGDERRILFGTGQWLHAIDARTGKLIESFGEKGRV
                     DLSQGLGRDVTGLAIQANTPGVVYRDLIIMGMRLGEGPAPAAPGHVRAYNVRTGELVW
                     RFHTLPQPGEPGYETWPEDAYQRVGGVNVWTGMTVDEERGLVFCPVGSAAFDFWGGNR
                     VGDNLYANCLLVLDAATGKRVWHYQFVKHDIWDRDPPAPPNLLTVTRDGQKIPAVAQV
                     TKSGHVFVFHRETGEPLFPIEEVMVPTSDLAGEVTSPTQRVPVKPKPFARQLFTADEI
                     TNRTPEARRAVLERFARLRPHTLFQPPSREGTIIFPGFDGGAEWGGAAVDPDGVLYVN
                     SNEMPWILAMIDASGATSLGQQVYLQNCTGCHGANREGNVAASIPSLHGLNERLTREQ
                     TFEVVTNGRNVMPPWGFLSEAQRHAVVGFIRGEGETPPPARSNDQEEQAADGVEKEKV
                     SEAEWTTYVADQEKPGYEEPPFTHTGYNRFLDPDGYPAVRPPWGTLNAIDLNTGEYLW
                     KVTLGELPELTAQGIPPTGTENYGGPVVTAGGVIFIGASKDEHFRAFDRKTGRELWKA
                     KLPAGGYATPATYEANGRQFVVIACGGGKMGTPSGDAYVAFALPRVR"
BASE COUNT          498 a         1068 c          653 g          489 t
ORIGIN      
        1 cacccgccgt atccgccgca ccagcacgcc atcccttcac cgccgccaac gccacggcgc
       61 cgcctgcgag cgttttcaca aattcacgac gcgaaacaga aagagaaacc aagtccttca
      121 tgaagtagaa caaacgcctg cccgacgaaa aaggatcacg caccgacgca aacgatcacg
      181 aatttcgatc aggccgactt cgctgccgtt tcattcgtgt ccattcgtgg ttcaaaatat
      241 tctcagcgca gcagccacgc ctccgcttcg catccctgtc catctgtgtt catccgtggt
      301 ttaccgaacc cgaggcaacg cgaacgccac atacgcatcg ccgctcggcg tgcccatctt
      361 tccgccgccg cacgcgatca cgacgaactg gcgcccgttc gcctcatacg tcgccggcgt
      421 cgcataacca cccgcgggca atttcgcctt ccacagctcg cgtcccgtct tccgatcgaa
      481 cgcccggaaa tgttcgtcct tgctcgcgcc gataaaaatc acgccgcccg ccgtcaccac
      541 cgggccgcca taattttccg tgcccgtcgg cggaatcccc tgcgccgtca attccggtaa
      601 ttcgccgagc gtcaccttcc aaagatattc ccccgtgttc agatcgatcg cgttcaacgt
      661 gccccacggc ggacgcaccg ccggataacc gtccggatcc agaaaccggt tgtagcccgt
      721 atgcgtgaac ggcggctctt cataacccgg cttctcctgg tctgccacat acgtcgtcca
      781 ctccgcttcg ctcaccttct ctttctcaac gccatccgcc gcctgctcct cctgatcgtt
      841 gctgcgcgcg ggcggcggcg tctcgccctc gccacgaatg aaacccacca ccgcatgccg
      901 ttgcgcttcc gacaaaaatc cccacggcgg catcacgttt cgcccgttcg tcaccacttc
      961 gaacgtctgc tcgcgtgtca gccgttcgtt caacccatgc agcgacggaa tgctcgccgc
     1021 cacattgcct tcgcgattcg ctccatggca gcccgtgcaa ttctgcagat aaacctgctg
     1081 gccgagcgag gtcgccccgc tcgcatcgat catcgccaga atccacggca tttcgttgct
     1141 gttcacatac agcacgccgt ccggatcgac tgccgcgccg ccccactccg ccccgccgtc
     1201 gaatcccgga aaaataatcg tcccttcccg gctcggcggc tgaaacagcg tatgcggtcg
     1261 cagtcgcgcg aaccgctcca acaccgcccg ccgcgcctcc ggcgtgcggt tcgtgatctc
     1321 gtccgccgtg aacaactgcc gcgcaaacgg cttcggcttc accggcacgc gctgcgttgg
     1381 cgacgtcact tcgcccgcca gatccgacgt cggcaccatg acctcttcga tcggaaacaa
     1441 cggctcgccc gtctcccgat gaaacacgaa cacatgcccc gacttcgtca cctgcgccac
     1501 cgccggaatc ttttgtccgt cgcgcgtcac cgtcagcaga ttcggcggcg ccggcggatc
     1561 gcgatcccag atgtcgtgct tcacaaattg ataatgccac acgcgtttcc ccgtcgccgc
     1621 atcgagcacc agcaggcagt tcgcataaag attgtcgccc acccgattgc cgccccagaa
     1681 atcgaacgcc gccgacccca ccggacaaaa caccagcccg cgctcctcgt ccaccgtcat
     1741 gcccgtccac acattcacgc cgccgacgcg ttgatacgca tcctccggcc aggtttcgta
     1801 gcccggctcg cccggctgcg gcagcgtgtg aaaccgccac acgagttcgc ccgttcgcac
     1861 attgtaggcg cgaacgtgac cgggcgccgc cggcgccggc ccttcaccga gccgcatgcc
     1921 catgatgatc aaatcccgat acaccacccc cggcgtgttc gcctggatcg cgagccccgt
     1981 cacgtcgcgt cccaaacctt gcgacaaatc cacgcggcct ttctcgccaa agctctcgat
     2041 caacttcccc gtgcgcgcat cgatcgcgtg cagccattgt cccgtgccga acaaaatccg
     2101 ccgctcgtct ccactctgcc aatacgcgag accgcggttc accccgttcg attccaccgg
     2161 ttcgaaccgc cacaactccc gccccgtcgc cgcatcgagt gcgaacactt tcaaccgcgg
     2221 cgtcgtgccg taaagcacgc catcgatcac cagcggatta cattggatct gcgtctgtcc
     2281 cgacgcatcg cccgcgttga aggtccacgc cacttcgagc tgcgcgacgt tctccggcgt
     2341 gatctgcgcc agcgtcgaat aatgactcgc cgcctggtcc ccaagataaa cccgccagtc
     2401 gcgatcggcc gccgcatgta gcggcgaaag ggaaacagcc acaccgatca gcaacaatgc
     2461 cgcgatccgc ggccaaaagg ggttttgcat cgcgacagtt gagcgccgcc cccgcccctt
     2521 gccaagcgga gagcaccgcc tgcttgtttt cttggagcgc ggcgcccccc gaccccgccg
     2581 tgttctcccg cccgttccac aaagagcctt cctccccttg ctcccgaggc cccctcctcc
     2641 ccacacgccc ccttgcctca ccgccccttc aaccaccccg acgccgccat ccattccgtc
     2701 acgcggtc
//
LOCUS       DASPLT010001556         3391 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_142510, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001556 DASPLT010000000
VERSION     DASPLT010001556.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 3391)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3391)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3391
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_142510"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..646
                     /locus_tag="VEA63_15845"
     CDS             <1..646
                     /locus_tag="VEA63_15845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HYD85540.1"
                     /translation="AYASFCSMKAMSTRNDSPQTASRPFDKNRDGFIMGEGAGILVLE
                     SLEHAQARGARIYCELAGYAATCDAFHITQPDPDGKGLSMAMKRALASAAVAPETVDY
                     INAHGTSTPYNDKFETLAIKKVFGDHARKVAVSSTKSMTGHLLGAAGGIESVISAKTI
                     QTGMIAPTINLQEPDPDCDLDYVPNQARAAKVRTVLSNNLGFGGQNAAVVFRAL"
     gene            complement(807..1037)
                     /gene="rpmB"
                     /locus_tag="VEA63_15850"
     CDS             complement(807..1037)
                     /gene="rpmB"
                     /locus_tag="VEA63_15850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HYD85541.1"
                     /translation="MARICAITGRRPTKGSIINRKGQSKKSGGIGTHVTSITKRKFRP
                     NLQRIRVKLPNGGTKRMLVSVKAIKAGLVTKA"
     gene            complement(1152..2156)
                     /locus_tag="VEA63_15855"
     CDS             complement(1152..2156)
                     /locus_tag="VEA63_15855"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HYD85542.1"
                     /translation="MSDQPPVSDEFRALFHAAAHASSGVLPFDKFMELALYHPEVGYY
                     RRDRPRIGYGGGTDFFTASTSGAVFGELVAAACTSLVGAEHASSYHFVEIGAETEAGV
                     LAGVAHPFASARTIRVDAEVAQLSGRCIVFSNELFDAQPFRRFIFHDNAWREIGVTFR
                     GERLVETELPPTEPPADFPSSAPERYVIDAPFAAGGLARRIAEEPWHGLFVAFDYGKR
                     WTEIAHECPAGTARAYYRHTQSNDLLARPGEQDLTCHICWDWLADALVQLGFSTPTVE
                     SQEAFFIRRAAEFIAAASTADAAHFSRRKLSLLQLLHPSHLGQKFQVLHAQRSPAPSS
                     "
     gene            2194..>3391
                     /locus_tag="VEA63_15860"
     CDS             2194..>3391
                     /locus_tag="VEA63_15860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HYD85543.1"
                     /translation="MHTVASMFKRIPVLAAGAALGFVLTFGAARSMQTWGWLPNRELN
                     RSADYMKDVLQMVNEYYIDAESVGYDRLAKTALHEMVESLDPHSEFLEARDYQDLEEQ
                     LSGDFTGVGIQVEMREGKVRVIAPIANSPAERAGIQRGDEILGVDGKGIEDKSSMGTV
                     VERLRGKPRTRVTIDLYRPTAERKFQVTLQRQVIKLESVRSVQVLPERTGYILLSEFS
                     EHTGREFGEALERLLKEGIDGLVLDLRNNPGGLLDAAVAVAEPFFRRGELIVSTRGRK
                     SADNESFRAELSGEPLALPMVVLINAGTASAAEVVTGALKDTGRAIVIGERSFGKGSV
                     QSVFELKNGEGLRLTTARYYSPAGLSIHETGIEPNVEVIATADEDSKLQVQHARADVK
                     DPKEFKE"
BASE COUNT          689 a          874 c         1229 g          599 t
ORIGIN      
        1 cgcgtatgcg agtttctgtt cgatgaaggc gatgagcacg cgcaacgatt cgccgcagac
       61 ggcgagccgg cccttcgaca agaatcgcga cggtttcatc atgggcgaag gtgcagggat
      121 tctcgtgttg gaatcgctcg agcatgcgca agcgcgaggc gcgcggatct attgcgaact
      181 cgcaggctat gcggcgacgt gcgacgcgtt tcacatcaca caaccggacc cagatgggaa
      241 ggggctttcg atggcgatga agcgtgcact cgcgagcgcc gcggtggcgc cggagacggt
      301 cgattacatc aacgcgcacg gcacgagcac gccttacaac gacaagttcg aaacgctcgc
      361 gatcaagaag gtcttcggtg atcacgcgcg gaaagtcgcg gtgagttcga cgaagtcgat
      421 gaccgggcat ttgctcggtg cggcgggagg aatcgagtcg gtgatttccg cgaagacgat
      481 tcagacgggc atgattgcgc cgacgatcaa tcttcaagag cccgatccgg actgcgatct
      541 cgactacgtg ccgaatcaag cgcgcgcggc aaaggtgcgg acggtgttga gcaacaatct
      601 cgggttcggc ggccagaacg cggcggtggt gtttcgcgcg ttataagaga tcgggctgcg
      661 gacgatcggg cacgtcgatc gcctgcgagg cgtcatatcg cctgcaagca ggcttctacc
      721 ttcgatccgg catagccgtt taggtgcgga cacaaaaaag cccgcggaga agatccgcgg
      781 gcgaaagaaa gggggagcgg gatcgctcag gccttcgtga ccaagccggc cttgatggcc
      841 ttcacggaga cgagcatgcg cttcgtgcca ccgttcggga gcttcacgcg gatgcgttga
      901 agattcgggc gaaacttccg cttcgtgatg ctggtcacgt gcgtgccgat gccgccgctc
      961 ttcttggact gacccttgcg gttgatgatc gagcccttgg tggggcggcg accggtgatt
     1021 gcacagattc tagccatgat gatttcgagt tggttaaagg gtcgagagtg aaggccggcg
     1081 ccgaggtgag gcaaggggat ttttgcgcgc cggcagaatg cgcgagagtg gtcacgcggc
     1141 ggccgaaaat gttacgacga aggtgcgggg gagcgttgag cgtgcagcac ttggaatttt
     1201 tggccgagat ggctgggatg aagtagctga agcagcgaga gcttgcgccg gctgaagtgc
     1261 gcagcgtcgg cggtgctggc ggcggcgata aactcggcgg cgcggcggat gaaaaaggcc
     1321 tcctgcgact cgacggtcgg agtgctgaaa ccgagttgga cgagcgcatc ggcgagccaa
     1381 tcccagcaga tgtggcacgt gaggtcctgt tcgccgggcc gggcaagaag gtcattggat
     1441 tgcgtgtgac ggtagtaagc gcgcgcggtt ccggcgggac attcgtgggc gatctcagtc
     1501 cagcgtttac cgtagtcgaa ggcgacgaag aggccgtgcc acggctcctc tgcaatgcgt
     1561 ctggcgagcc cgccagcggc gaacggcgcg tcgatcacgt agcgttcggg agccgatgat
     1621 ggaaagtcgg ccggtggctc ggtgggaggg agttcggttt ccacgaggcg ttcgccgcgg
     1681 aaagtgacgc cgatttcgcg ccaggcgttg tcgtgaaaaa tgaagcggcg aaagggttgg
     1741 gcgtcgaaaa gctcgttgga aaaaacgatg caacggccgg acagctgagc gacctcggca
     1801 tccacgcgaa tcgtgcgcgc tgaggcgaag ggatgcgcga ctccggcgag gacgccggct
     1861 tcggtttcgg cgccgatctc gacgaagtga taagatgatg cgtgttcggc gccgacgagc
     1921 gacgtgcacg cggcggcgac gagttcgccg aagacggcgc cgctggtgct cgcagtgaaa
     1981 aagtcggtgc cgccgccgta gccgatgcgc gggcgatcgc gccgatagta gccgacttcg
     2041 gggtgataga gggcgagctc catgaattta tcaaacggga gaacgcccga cgacgcgtgg
     2101 gcggcggcgt gaaagagcgc gcggaattcg tcggagactg gcggctggtc ggacatgcgc
     2161 ggcggcgatg aacccattta caaaccgaag cggatgcaca cagtcgcttc gatgttcaaa
     2221 cggattccag ttctggcggc gggcgccgcg cttggtttcg tcctcacctt cggggcggcg
     2281 cgctcgatgc agacgtgggg ctggttgccg aatcgcgagc tgaaccgttc ggcggactac
     2341 atgaaggatg tcctgcaaat ggtgaacgag tattacatcg atgcggaaag cgtcggttac
     2401 gatcggctgg cgaaaacggc gttgcacgag atggtcgagt cgctcgatcc acactccgaa
     2461 tttctcgaag cgcgcgatta ccaggatctc gaggagcagt tgagcggcga ttttacgggc
     2521 gtaggcatcc aggtggaaat gagggaagga aaagtgcgcg tgatcgcgcc gatcgcgaac
     2581 tcgccggcgg aacgcgcggg cattcagcgc ggcgacgaga ttcttggcgt cgacggaaaa
     2641 gggatcgagg acaagtcgag catgggcacc gtagtcgagc ggttgcgcgg aaagccgcgg
     2701 acgcgggtga cgatcgattt atatcggccg acggcggagc ggaagtttca agtcacactg
     2761 cagcggcagg tgatcaagct ggagagcgtc cgttccgtgc aagtgctgcc ggagcgcaca
     2821 ggctatattc tgctgagcga gttttccgag cacacgggcc gcgaattcgg cgaggcgctc
     2881 gagcgcttgt tgaaggaagg gatcgacggg ctggtgctcg atctgcgcaa caatccgggc
     2941 gggctgctcg atgcggccgt ggcggtggcc gagccgtttt ttcggcgggg tgaattgatc
     3001 gtgtcgacgc gcggccggaa gtcggccgac aacgagagtt ttcgcgcgga gctttcgggc
     3061 gagccgctcg cattgccaat ggtggtgttg atcaatgccg gcacggcgag cgcggcagaa
     3121 gtggtcacgg gcgcgttgaa ggatacgggg cgcgcgattg tgatcggcga gcgatcattc
     3181 ggcaaaggtt cggtccaatc ggttttcgag ctgaagaatg gcgaaggcct gcggttgacg
     3241 acggcgcgtt actattcgcc ggcgggcttg agcattcacg agacggggat cgagccgaac
     3301 gtcgaagtca tcgcgaccgc ggacgaagac agcaaattac aggtgcagca tgcgcgagcg
     3361 gacgtgaaag atccgaaaga attcaaagag c
//
LOCUS       DASPLT010001557         1125 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_203693, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001557 DASPLT010000000
VERSION     DASPLT010001557.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1125)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1125)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1125
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_203693"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1125)
                     /locus_tag="VEA63_15865"
     CDS             complement(<1..>1125)
                     /locus_tag="VEA63_15865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD85544.1"
                     /translation="CDDYRARFATEEKAIYLLPMDTRQAVIRFLMQRFGEVFGAAPTA
                     IGSYILDAAALGFLHEEFPGVRTAIVSCFEEGIKMYHGNNRQWHLFSEGGPWGIYQPS
                     RRCHLCPAKDEADAVGIVALPHLNRDMVLSLTSRDDLFASHPANLIRAAANDGPRCDY
                     VLHFIDQWIDQLRYNDQVYYSLFVSPPWLTAGHPFATNPAESRALYGESLAYLKARKD
                     EGVVRDQTVSETGEWHRSRVPINRAEVNLWSDILCGSRRQTFWYVDARMRVAIDLNAG
                     GAICDLRPLVGQVDRNMGADTRNLWNGNYPYLISSEHRGGVVSGPRQTAEIRCRGASV
                     DLSESRTSGRVTRGADGVPVLKTEPVALAVGGVTVTVASVF"
BASE COUNT          226 a          386 c          322 g          191 t
ORIGIN      
        1 aaacaccgac gcgaccgtca cggttacgcc accgacggcg agcgcgaccg gttcagtctt
       61 aaggactggc acgccgtcgg cgccacgcgt cactcgcccg gacgtccggc tctccgacaa
      121 atcaacgctc gcgccacgac agcgaatctc cgccgtctgc cgcgggccgg acaccacgcc
      181 gccgcggtgt tcagagctga tgagatacgg gtaattgccg ttccaaagat tcctcgtatc
      241 cgcacccatg ttacggtcga cctggccgac cagcggccgc agatcgcaaa tggcaccacc
      301 ggcgttgagg tcgatcgcga cgcgcatacg cgcatcgaca taccagaacg tctgccggcg
      361 tgacccacag agaatgtcgc tccagagatt cacctcggcg cgattgatcg gcacgcgact
      421 gcggtgccat tcgcccgttt cgctcacggt ctggtcgcgc accacgccct cgtccttgcg
      481 cgctttcagg taagccagcg attcgccgta gagcgcccgc gactcggcgg gattggtcgc
      541 aaacggatgc cccgccgtca gccacggcgg gctcacgaac aggctgtaat aaacctggtc
      601 gttgtaacgg agctgatcga tccactgatc gatgaaatga agcacgtagt cgcaacgcgg
      661 accgtcgttc gccgccgcgc gaatgaggtt cgccgggtgg ctcgcaaaca ggtcgtcccg
      721 actcgtcagc gacaacacca tgtcgcggtt caaatgcggc agcgcgacga ttcccactgc
      781 atccgcttcg tccttggccg gacacaaatg gcagcgtcgc gacggttgat aaatgcccca
      841 cggcccgccc tcgctgaaga ggtgccactg ccggttgttg ccgtgataca ttttgatccc
      901 ttcctcgaag caactgacga tcgcggtccg cacaccggga aactcctcgt gcagaaaacc
      961 cagcgccgcc gcatcgagga tgtaggagcc gatcgccgtc ggagccgccc caaacacttc
     1021 gccgaaacgc tgcatgagaa accggatcac cgcctgccgc gtgtccatcg gcaacaggta
     1081 gatcgctttt tcctcggtcg caaaacgcgc gcgatagtcg tcaca
//
LOCUS       DASPLT010001558         1209 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_203750, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001558 DASPLT010000000
VERSION     DASPLT010001558.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1209)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1209)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1209
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_203750"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            565..>1209
                     /locus_tag="VEA63_15870"
     CDS             565..>1209
                     /locus_tag="VEA63_15870"
                     /inference="COORDINATES: protein motif:HMM:NF028442.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HYD85545.1"
                     /translation="MSFTLRSGPAVAAFISLAIFAASAGAQAPDPLELGFRTPPESAR
                     PRTWWHWTMSNVTKDGITKDLEWMKRSGIGGFMLADVNFGRGQTVDPKTPFGTPEWYE
                     AVRFAASEAERLGLEMGIFSSPGWSETGGPWVKPEQAMKKFVWSETVVEGGQRFSGKL
                     AAPPNNIGQIRDTGASYYSSESREAPFYADAAVVAYRTPEIELAATALSSLPVVT"
BASE COUNT          251 a          386 c          364 g          208 t
ORIGIN      
        1 caaaggtctg cccccacgct ccccagccgc cgaacgccgg ctcaccccac aacccttgca
       61 tttccgcgaa ctcatccgcc agccatggcg tcgcggtatc gataatctgc gccggcgcgc
      121 ggggcatcac cgcaagcaga gccgaagcga tcaacgctgc cggcaaaata cgaaacgacc
      181 gagtggagtg actggagtaa cagcacagac ggcgaagaaa cggcataagc agcgtagttt
      241 tattttagaa aaaaagggag tggcggcgga acgtagcgtc ggacgatttt cccgtcgtca
      301 gcttagcgat agcgaagcac ggacgctatc aggcggtttg ccatcccgac gctcggcaag
      361 ccactcagcg gagtggcgga aagccggcgc gacaaaaaag atgatgccga tcgctaatcg
      421 atggcgactg gcgcgggtgt gaacacctga ccttactgtc agcgcgcaga cgatttgaaa
      481 ttggcttcgc caaacccaag gagaggccgc ggcagccccc atcgcgtctg ccggctcgat
      541 tctgattcac ctccacacct caccatgagc tttactcttc gctccgggcc cgccgtcgct
      601 gcgttcatct cgctcgccat tttcgctgcg tcggcgggcg ctcaggcgcc tgatccgctc
      661 gagcttggtt tccgcactcc gcccgagtcc gcgcgtccgc gcacctggtg gcattggacg
      721 atgagcaacg tcacgaagga cggcatcacg aaagacctcg aatggatgaa gcgctccggc
      781 atcggcggct tcatgctcgc cgacgtcaat ttcggccgcg gccagaccgt cgaccccaaa
      841 accccgttcg gcacgccgga gtggtatgag gcggtgcgtt tcgctgcgag cgaagcggag
      901 cggctcggtc tcgagatggg gattttcagc tcgcccggtt ggagcgaaac cggcggccca
      961 tgggtgaaac cagagcaggc gatgaagaaa ttcgtttgga gcgaaaccgt cgtcgaaggc
     1021 ggccaacgtt tttccggcaa gctcgccgcg ccgccgaaca acatcggcca aatccgcgac
     1081 accggcgcga gctactattc gagcgagtcg cgcgaagcgc cgttttatgc ggatgccgcg
     1141 gtcgtcgcct accgcacgcc ggaaatcgaa cttgctgcaa ccgcgttgtc gtcattgccg
     1201 gtcgtcaca
//
LOCUS       DASPLT010001559         1393 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_58462, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001559 DASPLT010000000
VERSION     DASPLT010001559.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1393)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1393)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1393
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_58462"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..559
                     /locus_tag="VEA63_15875"
     CDS             <1..559
                     /locus_tag="VEA63_15875"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HYD85546.1"
                     /translation="ANGALRGWTLRLIGPWKTSQGGGGDAYRETLAAAAAKLPGRVEL
                     LEPIFDERQLVSHYRDAAVFVYPSLAEFGETFGLSVLEAMAAGCAPVVSALGCFGDFV
                     RPGENGLVFDHRAADPAGELSRTLQQVATSREFRERLRAAAWQTARSYTLPEVAARFI
                     EDFRNILEPLPAAGSYLNECLPNSR"
     gene            538..>1393
                     /locus_tag="VEA63_15880"
     CDS             538..>1393
                     /locus_tag="VEA63_15880"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016145.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide pyruvyl transferase family
                     protein"
                     /protein_id="HYD85547.1"
                     /translation="MPPQLSMNGALAQLLAEFRGREVVALLNRGNRGDGVIHMGGRQF
                     LSHIGIAHREFRETDDLGAMNGDVLLVYGAGALSRGTHSLPRVLPTIARRFQQIVILP
                     SSFDVTEPRVRDFAETWDEKYSVFCRELVSFDALRRIGAKPNVLLMGHDLAFHADLSE
                     WSMRPARGRAGLFRRDNEAAYGRLPRDLDAVEDASYGTDREPERLLDYVARFAEIHTD
                     RCHGAITGAMMGRRVFFYRNNYFKNQAIYDHSLSGLGNVRFVKRTPFSLGQFGRAMYW
                     GRVRPVEMK"
BASE COUNT          216 a          456 c          445 g          276 t
ORIGIN      
        1 ggcgaacggc gctttgcgcg ggtggacgct ccgtttgatc ggcccttgga aaacctcgca
       61 gggcggtggc ggtgacgctt atcgcgaaac gctcgcggcc gccgccgcga aactgcccgg
      121 ccgggtcgaa ttgctcgagc cgatcttcga cgagcgccag ctcgtgtcgc actaccgcga
      181 tgctgccgtg ttcgtttatc cgtcgctggc cgagttcggc gagacgttcg ggctctctgt
      241 gctcgaggcg atggcggcag gttgcgcgcc ggtggtttcg gcgctcgggt gtttcggcga
      301 ttttgttcgc ccgggtgaga acgggctcgt cttcgatcat cgcgctgccg atccggccgg
      361 cgagctgtcc cgcacgcttc agcaagtggc gacctctcgg gaatttcgcg aacgcctgcg
      421 ggcggccgca tggcagacgg cccgcagcta cacgctgccc gaggtcgccg cccgtttcat
      481 cgaggatttc cgcaacatct tggaaccgtt gcccgcagcc gggtcgtatt tgaacgaatg
      541 cctccccaac tctcgatgaa cggcgcgctc gcgcaactgc tcgcggaatt ccgcgggcgt
      601 gaagtcgttg ctctcctgaa ccgcggcaat cgcggcgatg gcgtgattca catgggcgga
      661 cggcagtttt tgtcgcacat cggcatcgcc catcgcgaat ttcgcgagac cgacgacctc
      721 ggcgcgatga atggcgatgt gctgctggtt tacggcgccg gcgcgctctc gcgcggcaca
      781 cattcgctgc cgcgcgtgct tccgacgatc gcgcggcgct tccaacaaat cgtcatcctg
      841 ccgtcatcgt tcgacgtcac cgagccgcgc gttcgcgact ttgccgagac gtgggacgaa
      901 aaatacagcg tgttctgtcg cgaactcgtc agcttcgatg cactgcgccg gatcggcgcg
      961 aaacccaacg tgctgctgat gggccacgat ctcgcgttcc acgccgatct gagcgaatgg
     1021 tcgatgcggc ccgcgcgcgg tcgcgccggc ctctttcgcc gcgacaacga agcggcttac
     1081 ggtcgactgc cgcgcgatct cgatgcggtc gaagacgcgt cctacggcac cgatcgcgag
     1141 ccggaacgac tgctcgacta cgtcgcgcgt ttcgcggaga tccacaccga tcgctgtcac
     1201 ggtgcgatca ccggcgcgat gatgggccgc cgcgtttttt tctatcgcaa caactacttc
     1261 aagaatcagg cgatctacga ccacagcctt tcgggtctgg gcaacgtgcg ctttgtgaaa
     1321 cgcacgccgt tctccctcgg ccagttcggc cgcgccatgt attggggccg cgtgcgcccg
     1381 gtcgaaatga aat
//
LOCUS       DASPLT010001560         1162 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_120575, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001560 DASPLT010000000
VERSION     DASPLT010001560.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1162)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1162)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1162
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_120575"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..864
                     /locus_tag="VEA63_15885"
     CDS             <1..864
                     /locus_tag="VEA63_15885"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HYD85548.1"
                     /translation="LRASTPANIEIATRLADDCPPVECDPSQVHQVLMNLGTNAVYAM
                     RDYGGTLVVAARPVQVDEPLRAIHPDLRPGPAVCLQVQDTGTGMDENLRTRIFEPFFT
                     TKPTGEGSGLGLAVVHGIVKSHGGAIEVDSAPGNGACFKVYFPASVGAAARLAPAPAV
                     AAAERGRGQSLLFVDDETAIAFAGSSILRRMGYSVTTFTSPVEALDEIKTRPNGFDAV
                     ITDLSMPRMRGLDLIREIRKLRPEAVTVLISGRLEPADIETARALGVSATLAKPFTAD
                     SLAHTLSELLG"
     gene            complement(931..>1162)
                     /locus_tag="VEA63_15890"
     CDS             complement(931..>1162)
                     /locus_tag="VEA63_15890"
                     /inference="COORDINATES: protein motif:HMM:NF014634.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0031388 - organic acid phosphorylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HYD85549.1"
                     /translation="CFDETSWRGKGPGAVASHAIALGKPVHVFAGRIGAGFSIRGLSA
                     HEISPRELPLMEALRETRPNLSRAIRRAFHAG"
BASE COUNT          211 a          360 c          391 g          200 t
ORIGIN      
        1 ttgcgcgcgt cgaccccggc caacatcgag atcgcaacgc gcctggccga cgactgtccg
       61 ccggtcgagt gcgatccgag ccaggttcac caggtgttga tgaacctcgg cacgaatgcc
      121 gtctacgcga tgcgcgacta cggtggcacg ctggtcgtcg cggcacgtcc tgtgcaggtg
      181 gacgagccac tgcgggcgat tcatccggac ctgcggcccg gaccggcggt ttgtctccag
      241 gttcaggata cgggaaccgg catggacgag aatctacgaa cccgtatctt cgagccgttt
      301 ttcaccacga agccgacggg ggaaggctcc gggctcgggc tggcggtggt gcatggaatc
      361 gtgaaaagcc acggtggcgc gatcgaggtc gacagcgctc cgggaaacgg cgcgtgtttc
      421 aaagtctatt tccccgcgtc ggtcggcgcg gccgcccgct tggctccggc tccggcggtc
      481 gcggcggcag aacgcgggcg cgggcagagt ctcctgtttg tcgacgacga aaccgcgatc
      541 gcgttcgcgg gcagctccat tttacggagg atgggctaca gcgtcaccac gttcaccagt
      601 cccgtggagg cgctcgacga gatcaaaacg cgtccgaacg gtttcgatgc ggtgatcacc
      661 gatctcagca tgccgcgcat gcgcggcttg gatctgattc gtgagattcg caaattgcga
      721 ccggaagcgg tgacggtgtt gatcagcgga cgactggaac cggccgacat cgagaccgcg
      781 cgcgcgcttg gagtatccgc caccctggcg aagcctttca cggctgattc gctggcgcac
      841 acgctgagcg agttgttggg gtgaaacggc gagagcgcgc ccctcgaggc acgttcaacg
      901 agagccacga caggcacccg cgattgcgat tcagccggcg tgaaacgcgc ggcggattgc
      961 gcgcgagaga ttcgggcgtg tctcgcgcaa agcttccatg agaggtaatt cacgcggcga
     1021 gatttcatgc gcgctcaagc cgcggattga aaagcccgcg ccgatccgtc cggcgaaaac
     1081 gtgcaccggc tttccgagcg cgatcgcgtg cgaagccact gcgcctggcc ctttgccgcg
     1141 ccacgaagtt tcgtcgaaac ac
//
LOCUS       DASPLT010001561         1039 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_120620, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001561 DASPLT010000000
VERSION     DASPLT010001561.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1039)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1039)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1039
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_120620"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..444)
                     /locus_tag="VEA63_15895"
     CDS             complement(<1..444)
                     /locus_tag="VEA63_15895"
                     /inference="COORDINATES: protein motif:HMM:NF012900.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD85550.1"
                     /translation="MVTVKICGVRRIEDALLAADAGADEIGLLVGQRHPSGDFIAVEA
                     AAEIVAAIATRVTPVLVTHVSDPDEVHALARAAGVKDIQVHSDMSPAGLARLRELGGY
                     RLRKSWHVTGPESLGYGEAYRGVADSFLLDSMNAATGQVGGTGRAH"
     gene            576..>1039
                     /locus_tag="VEA63_15900"
     CDS             576..>1039
                     /locus_tag="VEA63_15900"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HYD85551.1"
                     /translation="MLSDLRLSLRALGKSRGFALIAILTLAVGIGTSTAIFSALRALV
                     ISPFHYPAADQLVHVWSGDGWTLSPADYLDVREQSSSFSAFGVYQPQSVNVGAENAQS
                     VMGISGTADVLRAFGVAPALGRWFDPSDDAVGAPPVVILSHALWQQLFAGDPL"
BASE COUNT          162 a          408 c          272 g          197 t
ORIGIN      
        1 gtgcgcgcgt cccgtgccgc cgacctggcc cgtcgccgcg ttcatcgaat cgagcaaaaa
       61 cgaatccgcc acaccgcgat aagcttcacc atagcccagg ctctccggcc cggtcacgtg
      121 ccaggatttc cgcagacgat atccgccgag ctcgcgcaaa cgcgccagtc ccgccggcga
      181 catgtcgctg tgaacttgaa tatctttcac gcccgccgcg cgcgccaacg cgtgcacctc
      241 atccggatcg ctcacgtgcg tcaccagcac cggcgtcact cgcgtagcga tcgccgccac
      301 gatctccgcc gcggcttcga ccgcaataaa atcgccgctc gggtgtcgtt gtcccaccaa
      361 cagaccgatc tcgtccgctc ccgcgtccgc cgccagcaac gcatcctcga tccgtcgcac
      421 gccgcagatt ttcacggtga ccatcgcgcc agaagaaaca tcgcccgctc cggcgcaacg
      481 tccgagtcca ccgccgttgc acacgtcgcg tcgaatcatc accaaacaat tttcgtaacg
      541 tttcgcccgc ccctgggttc ttgcctcaac tctccatgct ctccgacctc cgtctctcgc
      601 tgcgagcgct cggcaaatct cgcggctttg cgctgatcgc catcctcacg ctcgccgtcg
      661 gcatcggcac gtccaccgcg atcttcagcg ctctccgcgc gctcgtcatt tccccatttc
      721 attaccccgc cgccgaccag cttgtgcacg tgtggtcggg cgacggctgg acgctctcgc
      781 ccgccgacta cctcgatgtt cgcgagcaat cctcgagttt cagcgcgttc ggcgtttatc
      841 aaccgcagtc ggtcaacgtc ggcgcggaaa acgcgcagtc cgtgatgggc atcagcggca
      901 ccgccgacgt gctgcgcgcg ttcggcgtcg cgcctgccct cggccgctgg ttcgacccgt
      961 ctgacgacgc cgtcggcgcg ccgcccgtcg tcattctcag ccacgctttg tggcaacagc
     1021 ttttcgccgg cgatccgct
//
LOCUS       DASPLT010001562         1868 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_243310, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001562 DASPLT010000000
VERSION     DASPLT010001562.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1868)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1868)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1868
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_243310"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..184)
                     /locus_tag="VEA63_15905"
     CDS             complement(<1..184)
                     /locus_tag="VEA63_15905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD85552.1"
                     /translation="MAHAVDPTIKTRLLLRPVKSPLGTIRLSGINQGGFGVGRPPDPP
                     RTLQGCYGFTYIMNGSG"
     gene            286..1326
                     /locus_tag="VEA63_15910"
     CDS             286..1326
                     /locus_tag="VEA63_15910"
                     /inference="COORDINATES: protein motif:HMM:NF012576.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HYD85553.1"
                     /translation="MSSAPQITQRDVARACGVHPSTICLALKNSPSIPLPTRLRVQAA
                     AEELGYRPNVAARNLALLRGDRQGAGSLPIAWINQELQRNHWRSDVEAARHFDSARRR
                     AEELGYHLEEIWAREPGMTAGRLVQIVRARGIEGVIFPVHHSFEFSLLTPAWSEFALV
                     GLNDHRLGEWVDVVCPDHYRNAETVFRQVRRFGCGRVGLAISAQHDAASNGMVHSCYL
                     RYQADLTPSDRIPACFLPDESEGKQMAFEEWLHAYQPNVVVCAESALVGRARNAGFNA
                     MWLGLGAGALPFDGGMDESAGEVAAAAVDCVVDKMRRFEKGMRESTRVHLLKSAWIEP
                     RVAPMEIETVVA"
     gene            1323..>1868
                     /locus_tag="VEA63_15915"
     CDS             1323..>1868
                     /locus_tag="VEA63_15915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD85554.1"
                     /translation="MKTASRFAGAALVCVLTIGGLAVTEMRLHAADSAEATEALRVTF
                     VPTAQRLEIAAVGLTAGDYAITIETEELTQVETGDDVNEPGMIYLPPPEGKTEEEMKR
                     GAENVFFFNDLATADGWELVVRFDQEARGTWKLNGRFSSLGGAMEPEVTIPVDALEHG
                     ENLLHLRSADLSVPRMFFRKRF"
BASE COUNT          329 a          513 c          687 g          339 t
ORIGIN      
        1 ggccggagcc attcatgatg taggtgaagc catagcagcc ctgcagggtg cgcggcggat
       61 cgggcggacg gccgacgccg aagccgcctt ggttgatgcc ggagaggcgg atcgtgccga
      121 gcggggactt caccgggcgg agcaggaggc gcgttttgat cgtggggtcg acagcgtggg
      181 ccatgaaaaa ggggatacca ggaaacacat gctttctccc agttgtccca ttctgcaact
      241 cccaaagata agctagtgtg tccgccgtca gtccccaagg agtgcatgtc ttcagccccc
      301 caaattacgc agcgtgacgt cgctcgtgcc tgtggtgttc accccagcac gatttgcctc
      361 gcgctcaaga actctccctc gattccgttg ccgacgcgac tgcgagtgca ggcggcggcg
      421 gaggagttgg gttatcggcc gaacgtcgcg gcgcggaatc tcgcgttgtt gcgcggcgat
      481 cggcaggggg ccgggagcct gccgatcgcg tggatcaatc aggagttgca gcgcaaccac
      541 tggcggagcg acgtcgaagc ggcgcggcat ttcgacagcg cgcgtcggcg cgcggaagag
      601 ctcgggtatc atctcgagga gatttgggcg cgtgaaccgg ggatgacggc gggccggctc
      661 gtgcagatcg tgcgggcgcg cggcatcgag ggggtgattt ttcccgtgca ccacagcttc
      721 gagttctcgt tgctcacgcc ggcgtggagc gagttcgcgc tggtgggatt gaacgatcac
      781 cggctcggcg agtgggtgga tgtcgtttgc ccggatcact accgcaacgc cgaaacggtt
      841 tttcgccagg tgcggcgttt cggttgtggg cgcgtcgggc tcgcgatcag cgcgcaacac
      901 gatgcggcga gcaatggcat ggttcacagt tgctacctgc gttatcaggc ggatctcacg
      961 ccgtcggatc ggatcccggc gtgtttcctg ccggacgagt cggagggaaa gcagatggcg
     1021 ttcgaggaat ggctgcatgc gtatcagccg aacgtggtgg tgtgcgcgga atccgcgctc
     1081 gtcggccgcg cgcgcaatgc gggtttcaac gcgatgtggc tcggtctggg ggcgggagcg
     1141 ctgccgttcg atggcggcat ggacgaaagc gcgggcgagg tcgcggccgc ggcggtcgac
     1201 tgcgtggtcg acaagatgcg tcgcttcgaa aaaggcatgc gcgaatcgac gcgggtgcat
     1261 ttgctcaaga gcgcgtggat cgagccgcgc gtcgcgccga tggaaatcga aacggtcgtg
     1321 gcgtgaagac ggcgagccgt tttgcaggcg ccgcgctggt ctgcgtgctg acgatcggcg
     1381 gactcgccgt cacagaaatg cggctgcacg cggcggattc cgccgaagcg acggaagcgt
     1441 tgcgcgtcac tttcgtgccg acggcgcaac ggctggagat cgcggcggtg ggcctgacgg
     1501 ccggcgatta cgcgatcacg atcgagacgg aggaactcac gcaggtcgaa acgggcgacg
     1561 acgtgaacga acccgggatg atttatttgc cgccgccgga aggaaaaacg gaggaggaaa
     1621 tgaagcgcgg cgcggagaat gtttttttct tcaacgatct cgccacggcg gacgggtggg
     1681 agctggtggt ccgcttcgat caagaagcgc ggggcacgtg gaaattgaac ggccgctttt
     1741 cttcactcgg cggcgcgatg gagccggagg tgacgattcc cgtggacgcg ctcgagcatg
     1801 gagagaatct gctgcatctc cgcagcgcgg atttgagcgt gccgcggatg ttttttcgca
     1861 aacgcttc
//
LOCUS       DASPLT010001563         1085 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_203817, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001563 DASPLT010000000
VERSION     DASPLT010001563.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1085)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1085)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1085
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_203817"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..681)
                     /locus_tag="VEA63_15920"
     CDS             complement(<1..681)
                     /locus_tag="VEA63_15920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD85555.1"
                     /translation="MKTFPAWFAREPFDSRFRRRFVAASLLVVSIFSLTARAQQPPAP
                     WQSADIGDTAAGTTNYNSSTGVFTIEGGGDDIWGTADHFRYVYQTVTGDFSITAKVES
                     VENTYAWAKAGVMIRDGTNPGEPYAFAFMTPSTTNGGKFEYRSSGSSATGGAFDNTAL
                     PRWVRVTRAGNTFTAAQSVDGSTWNPIGTPQTIAMPSSVQIGLAVCSSVTNTLCTATF
                     SNVSVSNGL"
     gene            805..>1085
                     /locus_tag="VEA63_15925"
     CDS             805..>1085
                     /locus_tag="VEA63_15925"
                     /inference="COORDINATES: protein motif:HMM:NF014376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HYD85556.1"
                     /translation="MRAVYEVVRKPENSSLKLWYSTGYRFRAPWHFHPEVEILLVTRG
                     RGVRYVGDSVAAFHEGDLVMVGPDLPHVWISDSTSADDPRHVQCFIAQFR"
BASE COUNT          199 a          301 c          371 g          214 t
ORIGIN      
        1 aagaccgttg ctcaccgaca cattggagaa cgtggcggtg cagagtgtgt tggtcacgga
       61 cgagcacacg gcgaggccga tctggacgga agatggcatc gcgatcgttt ggggtgtgcc
      121 gatcgggttc caagtggagc cgtcgaccga ttgcgcggcg gtgaaggtgt tgccggcgcg
      181 cgtcactctc acccagcgcg gcaacgccgt gttgtcgaac gcaccgccgg tcgccgacga
      241 gccgctggag cgatactcga acttgccgcc gttcgtcgtc gaaggcgtca tgaacgcgaa
      301 cgcatacggc tcacccgggt tcgttccgtc acggatcatc acgccggcct tcgcccaagc
      361 gtaagtgttt tcgacgctct cgaccttggc ggtgatggag aagtcgcccg tgacggtttg
      421 gtaaacgtag cgaaagtgat cggccgtgcc ccagatatcg tcgccaccgc cttcgatggt
      481 gaacacgccg gtggacgaat tgtaattcgt ggtgccggca gcggtgtcgc cgatgtcggc
      541 ggactgccac ggggcgggcg gttgctgcgc gcgggcggtg agtgaaaaaa tggatacaac
      601 gagcagcgaa gccgcgacga accggcggcg gaagcgagag tcgaagggct ctcgcgcaaa
      661 ccaggcaggg aatgttttca tggggtatgt gtgcttcgtc cgacgacgct ctgatcggtc
      721 gagaacgagg cgcggcatta ttgacgaacg cgcgaattcc caccacgcgt cggtttggga
      781 aaaaccggca ctttttttaa ttttatgcgt gcggtctacg aagtcgtccg aaagccggag
      841 aacagctcgc tgaagctgtg gtattccacg ggctatcgtt ttcgagcgcc gtggcacttt
      901 catcccgagg tggagattct cctggtcacc cgcggtcggg gcgtgcgcta cgtcggcgac
      961 agcgtggcgg cgtttcacga aggcgatttg gtgatggtgg ggccggactt gccccatgtg
     1021 tggatcagcg actcgacgtc ggcggacgac cctcgccacg tgcaatgctt catcgcgcag
     1081 tttcg
//
LOCUS       DASPLT010001564         2340 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_203823, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001564 DASPLT010000000
VERSION     DASPLT010001564.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 2340)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2340)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2340
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_203823"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..625
                     /locus_tag="VEA63_15930"
     CDS             <1..625
                     /locus_tag="VEA63_15930"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HYD85557.1"
                     /translation="EIRPGAWLDARLALWLAGPRTLVVADLHWGYATSHRARGNLLPI
                     WGDEEIAARLHALIADYRPADMIWLGDSLHTLAGRNAAESFLQTATVPIEIVAGNHDA
                     KWNLVSAPAVVMRDGYCLHHGDLPRKVPPDSVEIIGHHHPAATWSDGAGTRLKLPALI
                     VSRRRIVLPAFSPWAAGTPWLASTPDETVYAIGTKRIFTLSPDARQK"
     gene            622..1425
                     /locus_tag="VEA63_15935"
     CDS             622..1425
                     /locus_tag="VEA63_15935"
                     /inference="COORDINATES: protein motif:HMM:NF022750.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HYD85558.1"
                     /translation="MTLRMMRFVFFACLALSSVAVAAAAQAPLPLQEGEQLTYRVAWG
                     IFGRAGEIKIEAATETNDGVPNLVVTTTTQTRGVLKRLFPFEARGESIFELPSGRLML
                     LTEASESGKKKTNSAISFNYETRTARYTDFLDSKKNADIEIPPGEDLRDLITSLVQTR
                     MWELKPGEKRDINVVFEDDIYQLTVHALGYEVIETEIGRFNTLMLEPRMEKTPPKGMF
                     RRGSRVHVWIAQDDPRRLPVRFEVEFKFGAGIATIEAYTPPGGAPPSAD"
     gene            complement(1414..2019)
                     /locus_tag="VEA63_15940"
     CDS             complement(1414..2019)
                     /locus_tag="VEA63_15940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HYD85559.1"
                     /translation="MQRLIKAEELSARKTVATARWLLGKHLVRRRADGTEEARVITEV
                     EAYNGERDLACHARAGCTARTAVLYRAAGVWYVYLCYGIHEMLNLVVGPPGWPAAVLI
                     RGVDGISGPGRVTKRLEIDRKLNGAAAEPASGLWLEDRGLRVPRNALEITPRIGVDYA
                     GEIWAAKPWRFVVKDLAALKERAGRASEGESGRVARATSVG"
     gene            2134..>2340
                     /locus_tag="VEA63_15945"
     CDS             2134..>2340
                     /locus_tag="VEA63_15945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD85560.1"
                     /translation="MPIVTMSLPVNVFMTALVAKAFPLPKMTIGLISALPFIGNFLQI
                     FAAPFLARWRPPKTITAIAASLHLV"
BASE COUNT          429 a          837 c          650 g          424 t
ORIGIN      
        1 ggaaatccgc cccggcgcgt ggctcgatgc gcggctcgcg ctgtggctcg ctggcccgcg
       61 cacactcgtc gtcgccgatt tgcactgggg ctacgcgacc agccatcgcg cgcgcggcaa
      121 tcttctcccg atctggggcg acgaagaaat cgccgcgcgg ctgcacgcgc tcatcgccga
      181 ttatcgtccc gccgacatga tctggttggg cgattccttg cacacgctcg ccggacgcaa
      241 cgcggccgag tcgtttttgc agacagcgac ggttccgatc gaaatcgtcg caggtaatca
      301 cgatgcgaaa tggaacctcg tgtccgctcc cgcagtcgtc atgcgcgatg gttactgcct
      361 gcatcatgga gacttgccgc gcaaagtgcc gccggactcg gtggaaataa tcggccatca
      421 tcatcccgcg gccacttgga gcgatggcgc cggcacgcgg ctcaagcttc cggcgctgat
      481 cgtctcgcga cgccgcatcg tgttgccggc gttttctccg tgggccgccg gcacgccgtg
      541 gctcgcttca acgcccgacg aaacggttta cgccatcggc acgaaacgaa tctttacact
      601 ctcacccgac gctcgccaga aatgaccctt cgcatgatga gattcgtctt cttcgcctgt
      661 ctggcattgt ccagcgttgc ggtcgccgcc gccgctcagg cgccgctgcc cctgcaggaa
      721 ggcgaacagc tcacgtatcg cgtcgcgtgg ggaatcttcg gccgcgccgg cgagatcaag
      781 atcgaagccg ccacggaaac caacgacggc gtgcccaacc tggtcgtcac gaccaccacg
      841 caaacgcgcg gcgtgttgaa gcgcttgttc cccttcgaag cccgcggtga atcgattttc
      901 gaactgccca gcggtcgctt gatgctgctc accgaagcga gcgagagcgg caaaaagaaa
      961 accaacagcg ccatctcgtt caattacgag acccgcaccg cacgctacac cgactttctc
     1021 gattcaaaaa agaacgccga cattgagatc cctcccggtg aagacctgcg cgatctcatc
     1081 accagcctcg tgcaaacccg catgtgggag ctgaagcccg gcgaaaagcg cgacatcaac
     1141 gtcgtcttcg aggacgacat ctaccaactc acggttcatg cgctcggcta cgaagtcatc
     1201 gaaacagaga tcggccgttt caacacgctg atgctcgagc cccgcatgga aaaaacgccg
     1261 ccgaaaggca tgttccgccg cggctcgcgc gttcacgtgt ggatcgcgca agacgacccg
     1321 cgccgcctgc ccgtgcgctt cgaggtcgaa ttcaaattcg gcgccggcat cgccacgatc
     1381 gaagcctaca cccctcccgg cggcgccccg ccgtcagccg actgacgtag cgcgggcaac
     1441 ccgcccgctc tccccctcgc tcgcacgccc cgcacgttcc ttcagcgccg cgaggtcctt
     1501 cacgacaaac cgccacggct tcgccgccca gatctctccc gcgtagtcga caccgatgcg
     1561 cggcgtgatc tcgagcgcgt tccgcggcac gcgcagtccg cgatcctcca gccaaagtcc
     1621 actcgcaggc tccgccgccg cgccatttag ctttcgatcg atttccagcc gcttcgtcac
     1681 gcgaccgggt ccggaaattc cgtcaacgcc gcgaatcaac accgctgccg gccagcccgg
     1741 cgggccaacg acgaggttca acatctcgtg aatgccgtag cagagataaa cataccacac
     1801 gccggcggcg cggtataaga cagcggttcg cgccgtgcag cccgcgcggg cgtgacacgc
     1861 cagatcgcgt tcgccgttgt aggcttccac ctcggtgatt acgcgcgcct cctccgttcc
     1921 atcggcgcgt cgacgcacca gatgcttgcc cagaagccac cgcgccgtgg caaccgtttt
     1981 gcgcgcgctc aattcttcgg ctttgattaa ccgctgcatc tttccttgat ttgatctttt
     2041 ccggtgaacg caactcccgc gcgcgaggcc gcccaacgca aagccgatct ccttcaatcc
     2101 ctgcgcctgt gcacgcgcga gggaatcgtg gccatgccga tcgtgaccat gtcgcttccg
     2161 gtgaacgtct tcatgacggc gctcgtggcg aaagcgtttc cgctgccgaa gatgacgatc
     2221 ggcctgatca gcgcgctgcc gttcatcggg aatttcctgc aaattttcgc cgcgccattt
     2281 ctcgctcgct ggcgcccgcc gaaaacgatc accgccatcg ccgcctcgct ccacctcgtc
//
LOCUS       DASPLT010001565         1075 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_223388, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001565 DASPLT010000000
VERSION     DASPLT010001565.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1075)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1075)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1075
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_223388"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..223
                     /locus_tag="VEA63_15950"
     CDS             <1..223
                     /locus_tag="VEA63_15950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD85561.1"
                     /translation="FTMVAQIALFMLSMQIVLHAYGAFFATLPFFVVGALGMYWFWWR
                     HLPGASAADDATALSDDVAVTAKAAGRRS"
     gene            646..>1075
                     /locus_tag="VEA63_15955"
     CDS             646..>1075
                     /locus_tag="VEA63_15955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Plug domain-containing protein"
                     /protein_id="HYD85562.1"
                     /translation="MKRDPRLTFITGALLAVAWLPPLFGQAASPAPAEPPEDDIVTLS
                     PFVVSSETERGYQATETLAGMRIKTNLKDTAAAIDVLTDQFLNDVGATDMFDALKYVA
                     NMEYASYQASDQSNASQWFSSSYISRGISGSTVLLDFFPTG"
BASE COUNT          192 a          321 c          335 g          227 t
ORIGIN      
        1 gttcacgatg gtggcgcaaa tcgcgttatt catgctgtcg atgcagatcg tgctgcacgc
       61 ttacggcgcg tttttcgcga cgctgccgtt cttcgtcgtg ggcgcgctgg gcatgtattg
      121 gttttggtgg cgccatctgc cgggtgcatc ggcggcggac gacgcgacgg cactgtctga
      181 tgacgttgcg gtgacggcca aagctgcggg gcgccgttcg tgatttcgcg gcgcaggcag
      241 tgtgcaaatt tcaccgtccg cggtgaagtg cgtggagcgt gtcgtttggg cgtgagccga
      301 aaagctcacg cttttcgcca tgtatgtatg aattcttgga gctgacggtc gcgcgacttg
      361 tgccgggcgg gcggggcgcc gatgttgccg tctctggata aaaaaacaca agtgttgccc
      421 aaaatcggac aagaaagttt tcgctgctct tcggtatggt gtgggcgtga gacacgaagc
      481 ggcgtcggcc gcggtcactc acgaccccag atgcaacccc acacgagtca gaccggatcg
      541 ctccggcaac gaggcggccg gttggaagga ccatcggttt tggtccgatc gtgatccgcg
      601 gcgttgctca gacccagccg tcagacctca acacccaaac acatcatgaa acgcgatccg
      661 cgtcttacgt tcatcaccgg cgccttgctc gccgtcgcct ggcttccgcc gcttttcggc
      721 caagccgctt cacccgctcc ggctgaaccg ccggaggacg acatcgtcac gctctcgccg
      781 tttgtcgtct catcggaaac ggaacgcggc tatcaggcga ccgagacgct cgccggcatg
      841 cgcatcaaaa cgaacctcaa ggacacggcg gcggcgatcg atgtgctcac cgatcaattt
      901 ctcaacgacg tcggcgcgac cgacatgttc gacgcgttga agtatgtcgc gaacatggag
      961 tatgcgtcgt atcaggcgag cgaccagagc aacgcgtcgc agtggttctc gtcgtcgtat
     1021 atctcgcgcg gcatctccgg cagcacggtg ttgctcgact ttttcccgac cggct
//
LOCUS       DASPLT010001566         2371 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_142674, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001566 DASPLT010000000
VERSION     DASPLT010001566.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 2371)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2371)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2371
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_142674"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..470
                     /locus_tag="VEA63_15960"
     CDS             <1..470
                     /locus_tag="VEA63_15960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD85563.1"
                     /translation="SPIFTTSRHRNAYWFNGFQPNATVALRLSFPLGCPLFVGTETLI
                     ERNRSVFRFSRAWRHECRIFIEQSETDGVVTVRDIPTQLPWRQRSLLVRGLKNATLRF
                     LPDTGVPTAEVVMLRQPRYPHLSGDFVQPQRIETPFGLVLEAHALSGDLLIAW"
     gene            491..1984
                     /locus_tag="VEA63_15965"
     CDS             491..1984
                     /locus_tag="VEA63_15965"
                     /inference="COORDINATES: protein motif:HMM:NF014649.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose/L-arabinose isomerase family protein"
                     /protein_id="HYD85564.1"
                     /translation="MIRTLDPITTSLDSAPILIPRRRPLTARVGIFGVGFHMYWPQFP
                     GLQEELLGHLKTFTNRVAAQNVQAIDFGMVDDAQSAYALLRKLKAADLDLVFCNMLTY
                     ATSSTFGAIIRELDIPVVLVALQPLARMDYSKATTYMQLCNDNICSLPEFAGVAVRMG
                     KKVPPTIVGTLHDDPKAESEIARWCGIARVLQDVRKARIGHFGHPIEAMLDMHTDPTA
                     ITAAFGLHVVQTEVDDLLRLSKLVTPSEVKEKEDEIRRVFDFPEPETDPLTMKLTDED
                     LRAAAHYAAVLDRFIDEFQLDGLTYYYKGEPGSEIERLASNLIVGNSLLCGAGFPMCS
                     ESDLKTCVSMLIFDRLEIGGSFTEFHPVDFVDDFILVGHDGPHHINVAAGRPVLRSLR
                     HYHGKTGRGASVEFKLKEGPITMMSITSNAQGKFKFVIGEGQSVHGRIPATGNTNTRG
                     HFEPSAREFLQRWVAAGPTHHFALGVGHHASTLKQIGDLLGIEAVIV"
     gene            complement(2130..>2371)
                     /locus_tag="VEA63_15970"
     CDS             complement(2130..>2371)
                     /locus_tag="VEA63_15970"
                     /inference="COORDINATES: protein motif:HMM:NF012956.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="discoidin domain-containing protein"
                     /protein_id="HYD85565.1"
                     /translation="PRNDGANIGFYFPIDFIIQTSPDGTTWTTRVTQTGYPQPGNSVQ
                     AFPFATVTARYVRVTATNLRFHTAYRMQFAELEVY"
BASE COUNT          452 a          758 c          672 g          489 t
ORIGIN      
        1 agtctccgat cttcaccacc tcgcgtcatc gaaacgccta ttggttcaac ggctttcagc
       61 cgaatgccac cgtcgcgttg cggctatcgt ttccgctcgg ctgcccgctg ttcgtcggca
      121 cggaaacact catcgaacga aaccgcagcg tgtttcgctt ctcgcgcgca tggcgtcacg
      181 agtgtcgcat cttcatcgag cagtcggaaa cggatggcgt cgttacggtg cgcgacatcc
      241 ccacacagct tccctggcgc cagcgcagct tgctcgtgcg cggattgaag aacgccacgc
      301 tgcgtttcct gccggacacg ggagttccca ccgcggaagt cgtgatgctc cgccagcccc
      361 gttatccgca tttgtcgggc gatttcgtgc agccgcaacg aatcgaaacg ccgttcgggc
      421 tcgttctcga agcccatgct ctctccggcg atctcctgat cgcgtggtga tccttcctct
      481 ttcccttatt atgattcgca ctctcgaccc catcactact tcgctggatt cggcacccat
      541 tttgattccg cgacgtcgtc cgctcaccgc ccgcgtcggt atcttcggcg tcggtttcca
      601 tatgtattgg ccgcaatttc ccggattgca ggaggaactg ctcggacacc tgaaaacatt
      661 caccaaccgc gtcgccgcgc aaaacgtcca ggcgatcgac ttcggcatgg tcgatgacgc
      721 gcagtccgcc tacgcgctgc tgcgaaagct gaaggccgcg gacctcgatc tggttttctg
      781 caacatgctc acctacgcga cgtcgtccac gttcggagcg atcattcgcg agctggacat
      841 tcccgtcgtg ctggtcgcac tccagccgct cgcgcgcatg gattactcga aagcgacgac
      901 ctacatgcag ctctgcaacg acaacatttg ctcgttgccg gagttcgccg gcgtggccgt
      961 gcgcatggga aagaaagttc ctcccacgat cgtcggaacg cttcacgacg atcccaaggc
     1021 cgagagcgag atcgcccgct ggtgcgggat cgcccgcgtg ctccaggatg tgcgcaaagc
     1081 gcgcatcgga catttcggcc atccgatcga agccatgctc gacatgcaca ccgatccgac
     1141 cgcgatcacc gcggccttcg gacttcacgt cgtgcaaacc gaagtggacg atctcctgcg
     1201 gctctcgaag ctcgtgacgc cgtcagaggt gaaagaaaag gaggacgaga ttcgtcgcgt
     1261 gttcgatttc cccgaacccg aaaccgaccc gctcacgatg aaactcaccg acgaggatct
     1321 tcgtgccgcg gctcactacg cggccgttct cgatcgcttc atcgacgaat tccaactcga
     1381 tggtctcacc tattactaca aaggcgaacc cggcagcgaa atcgagcggc tcgcgagcaa
     1441 cttgatcgtc ggcaactcgc tgctctgcgg cgcgggtttt ccgatgtgca gcgagtccga
     1501 cctgaaaacg tgcgtgtcga tgttgatctt cgaccgcctg gaaatcggcg gcagtttcac
     1561 ggagtttcac ccggtcgatt ttgtcgacga tttcattttg gtcggtcacg acggaccgca
     1621 tcacatcaac gtcgccgccg gacgtcccgt gttgcgcagc ctgcgtcatt atcacggcaa
     1681 aaccggccgc ggcgcttccg tcgagttcaa gctcaaggaa ggtccgatca cgatgatgag
     1741 catcacctcc aatgcgcagg ggaagttcaa atttgtaatt ggagaaggcc aatcggtgca
     1801 cggcagaatt cccgcgaccg gaaacaccaa cacgcgcggc cacttcgagc cgagcgcgcg
     1861 cgagttcctg caacgctggg tagccgcagg cccgacgcat cacttcgcgc tcggcgtcgg
     1921 acatcatgcc tcgacgctca agcaaatcgg cgacctcctc ggcatcgaag cggtgatcgt
     1981 gtaattggcc cgaaatcctt ctgcgccgag tcgcgctcat ccagcccgct cgttgagcgg
     2041 gcttttttct gcaaccgtca gccaaagccc tgcgctcacg cgcagggcta gtggattcgg
     2101 cgtagcccgc tccgtgagga gcgggttgtc taatacacct cgagctcggc gaactgcatg
     2161 cgataggccg tgtggaacct caggttcgtc gcggtcaccc gcacataacg cgcggtgacg
     2221 gtggcgaacg ggaatgcctg aaccgaattt cccggttgcg gatagccggt ttgcgtcacg
     2281 cgcgtggtcc aggtggtgcc gtccggcgag gtttgaatga tgaagtcgat cgggaagtag
     2341 aaaccgatgt ttgcgccgtc attgcgcgga t
//
LOCUS       DASPLT010001567         1788 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_183517, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001567 DASPLT010000000
VERSION     DASPLT010001567.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1788)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1788)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1788
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_183517"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1049
                     /locus_tag="VEA63_15975"
     CDS             <1..1049
                     /locus_tag="VEA63_15975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HYD85566.1"
                     /translation="NGVARFEIAAPQLALWSPEQPRLYTVELAAGDDRIAEKIGFRTI
                     ETRGTDILLNGESIFLRGISLHEENPLRGGRATTPADARILLGWAKELGCNFVRLAHY
                     PHNEHMARVADELGLMLWAEVPVYWTIAWENPDTLANAQNQLTELIVRDRNRASVIVW
                     SVANETPVSEPRTRFLKSLVDLARSLDNARLVSAAMEVETDPADDNHKIVEDPFGKYT
                     DLLSFNQYVGWYVGLPDKLQKIRWTLAYEKPVVISEFGADALQGLHADKLTRFSEEYQ
                     EDLYVQTLAMLQKIPAWRGTTPWILCDFRSPRRPLPHVQDGWNRKGLIAENGTKKKAF
                     YVLKKFYEEKAALK"
     gene            1052..1642
                     /locus_tag="VEA63_15980"
     CDS             1052..1642
                     /locus_tag="VEA63_15980"
                     /inference="COORDINATES: protein motif:HMM:NF024952.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2'-5' RNA ligase family protein"
                     /protein_id="HYD85567.1"
                     /translation="MNCASPQFDLPGFEERKPEPDVVFLALRPEPIAAQAAERLTRRY
                     CERLAGARPLATDRFHISLHALGPSYTLPPYFISMASAVVDEIEMPAFDVVLDRAMSL
                     NGSNGRLPLVLCESSGLSLVREFHRALGIALIKSGLSRHVRKQIKPHMTLLYHERRIA
                     EHPIEPVSWTANEFVLIRSLHGQTKHIELGRWPLRS"
BASE COUNT          387 a          589 c          473 g          339 t
ORIGIN      
        1 cgaacggcgt cgcccgcttc gagatcgccg cgccgcagct cgcactctgg tcgcccgagc
       61 agccgcgact ttacaccgtg gaactcgccg ccggcgacga tcgcatcgcc gaaaaaatcg
      121 gtttccggac aatcgaaacc cgaggcaccg acatcctcct caacggtgaa tcgattttcc
      181 tgcgcggcat ctcgctgcac gaagaaaatc cccttcgcgg cggccgcgcc accacacccg
      241 ccgacgcgcg tattctcctc ggttgggcga aggagctcgg ctgcaatttc gtccgcctcg
      301 cccactaccc gcacaacgag cacatggccc gcgtcgccga cgaactcggc ctgatgctct
      361 gggccgaggt gccggtctat tggacaatcg cttgggaaaa tcccgacacg ctcgccaacg
      421 cacaaaacca gctcaccgag ctgatcgtcc gcgaccgcaa ccgtgccagc gtgatcgtct
      481 ggtcggtcgc gaacgaaacg ccggtcagcg aaccgcgcac gcgtttcctc aaatcgctcg
      541 tcgatctcgc gcgctcgctc gacaacgcgc ggctcgtctc cgcggcgatg gaagtcgaaa
      601 ccgatcccgc tgacgacaat cacaagatcg tcgaggatcc gttcggcaaa tacaccgacc
      661 tgctcagctt caaccaatac gtcggctggt atgtcggcct gcccgataaa ctgcaaaaaa
      721 tccgctggac gctcgcctac gaaaaaccgg tcgtgatcag cgaatttggc gccgacgcat
      781 tgcaaggcct gcacgccgac aagctcacgc gtttcagcga ggaatatcag gaagacctct
      841 acgtgcaaac gctcgcgatg ctgcaaaaaa tccccgcctg gcgcggcaca acgccatgga
      901 ttttatgcga cttccgttca ccgcgtcgtc cgcttccgca cgtgcaagac ggctggaatc
      961 gcaaaggtct gatcgccgaa aacggcacga agaaaaaagc gttctacgtg ctgaaaaagt
     1021 tctacgagga gaaagccgca ctgaaatagt cgtgaactgc gccagccctc aatttgattt
     1081 gcccggcttt gaagagcgaa agccagagcc cgacgtcgtc tttcttgcgc tgcgccctga
     1141 gccgatagcc gctcaagcag cagagcgcct taccagacgc tattgtgagc gactagccgg
     1201 agctcggccg ctcgcgactg atcgttttca catcagcctt catgcactcg gtccgtctta
     1261 cacgctcccg ccttacttca tttcgatggc gagcgccgtg gtggacgaga ttgaaatgcc
     1321 tgccttcgat gtcgtgctag atcgagcgat gagtctaaac ggcagtaatg gccggcttcc
     1381 tttggtgctc tgtgaaagca gtggtctttc cttggtccgc gaatttcacc gcgctcttgg
     1441 aatcgcactg atcaaaagcg gtctttcgcg ccacgtcaga aaacagatca agccacacat
     1501 gacgctgctc tatcacgagc ggcggattgc tgaacatccg atcgagccag ttagctggac
     1561 cgcgaacgaa ttcgttctca tccgcagcct acacggacaa acaaaacaca ttgagctcgg
     1621 ccgttggccg ctgcgcagct aaagcgccct ccgccaatca ccgccactcg tgccgcggat
     1681 agcgccgcga caactccgcc ttcaccttcg gatactgttc gcgccagaaa ttcgtgaggt
     1741 cgtccgtcac ttgaatcggc cgctggtttg gcgcgagcac ctcgatct
//
LOCUS       DASPLT010001568         4053 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_243355, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001568 DASPLT010000000
VERSION     DASPLT010001568.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 4053)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4053)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4053
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_243355"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..372
                     /locus_tag="VEA63_15985"
     CDS             <1..372
                     /locus_tag="VEA63_15985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HYD85568.1"
                     /translation="PFKRVVDFYNSNQVKVVMRQDGRALYFSRSRMPFSRDLGLTIDD
                     TWVQANPCYRHLGLYAYRADLLEKFAKLPPGRYEQIEKLEQLRVLENGYDIACAVTED
                     PTIGVDTPEDAKRFEALLTAK"
     gene            complement(491..1663)
                     /gene="carA"
                     /locus_tag="VEA63_15990"
     CDS             complement(491..1663)
                     /gene="carA"
                     /locus_tag="VEA63_15990"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007360867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HYD85569.1"
                     /translation="MSPSKTGVLALEDGSVYRGLAFGADATIAGECVFNTSMTGYQEI
                     ITDPSYFGQIVTMTAVQIGNYGLSPEDEESGGPKCAGLIVRELSPVVSNWRSTISLHD
                     YLAKHGIPGLSEIDTRALTKKLRVDGAMKCCLSTLPISDEDAVARARAWHDMAGSDYV
                     KDVTCAVPYIWRADDLANHNAPYLPVGTTLNAPGIPAKKFRVAAFDYGAKHTIFRKLI
                     RHGFDVQVFPSTATAEQIREQNPDGVFLSNGPGDPAALDYVHKTVTGLLPHYPTFGIC
                     LGHQMITHALGGETFKLKFGHRGGNQPVKNLETGKVSITAQNHGFATDPKSLEKRGAK
                     VTEINLNDNTVEGLRHTSLPVFSVQYHPEAAPGPNDADPLFLDFYKMIEDRKAGKI"
     gene            complement(1849..2643)
                     /locus_tag="VEA63_15995"
     CDS             complement(1849..2643)
                     /locus_tag="VEA63_15995"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HYD85570.1"
                     /translation="MPPSAASVAPLRILLVEDQTLDAELMIMELKRSGYAAATVIRVE
                     NPEDFSRELGLEPDIILCDYALPTFSAPEALRIVQQRQLDIPFIVVSGSIGEEIAVEA
                     IKNGADDYLLKDRLGRLGAAVSQAVEEKKLRDAARRAEDDLRQSEYKYRCLFEHLPDA
                     AYLCDSATGRIIDTNRRGETLLGLDRAGVLGQRLSQFMPAGIFRSLLAIGQGTPEAPL
                     ELETEITAPLKRPIPVQINAGLVPIYNRQLLLAFFRSRRGEAAPHG"
     gene            complement(2756..3073)
                     /locus_tag="VEA63_16000"
     CDS             complement(2756..3073)
                     /locus_tag="VEA63_16000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD85571.1"
                     /translation="MKNILPLAACSVALFFFFATSAHAQTSSAASLADLARSHEQVFK
                     ELADEQTALVLQRGDKQFVIYGVTHVRAVGSAIEVTVKNGRKYSVNPADVFFLTNDAF
                     GLK"
     gene            3597..4001
                     /locus_tag="VEA63_16005"
     CDS             3597..4001
                     /locus_tag="VEA63_16005"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HYD85572.1"
                     /translation="MSGTLAAGRRILVVDDDDMVRDFLVGHLLRALPSAHVLTANDGT
                     SGLVSYRRELPDLIVTELNMHQMSGDELIEHIRKTDTTTPILVLTGAPETTPIPGATE
                     VVPKIRLGYLVQRVTDLLANSPRVEETRGSAR"
BASE COUNT          773 a         1058 c         1421 g          801 t
ORIGIN      
        1 cccttcaagc gggtggtgga tttctacaat tccaatcagg tgaaggtcgt gatgcggcag
       61 gatgggcgcg cgctttattt ttcacgttcg cggatgccct tctcgcgcga tctgggttta
      121 acgatcgacg acacgtgggt gcaggcgaat ccgtgctacc ggcatctcgg gttgtatgcg
      181 tatcgcgcgg acctgttgga gaaattcgcg aagctgccgc cggggcgcta cgagcagatc
      241 gaaaaactgg agcagttgcg cgtgctcgag aacggctacg acatcgcctg cgccgtgacg
      301 gaggatccga cgatcggcgt cgacacgccg gaggatgcga agcgcttcga ggcgctgctt
      361 acggccaagt aggaagtaac aagtatcaag taacaagagg gcggcagccg ggcagaaact
      421 ggcactgccg cgagccgcgg atcttgctac ttgctactta atacttgtcc cttccgcgcg
      481 aagcgcgggc tcagattttg ccggctttgc gatcctcgat catcttgtag aaatcgagga
      541 agagcgggtc ggcgtcgttg gggccgggtg cggcttcggg gtgatattgc acgctgaaga
      601 cggggagcga ggtgtggcgc agaccttcga cggtgttgtc gttgagattg atctcggtga
      661 cttttgcgcc gcgtttctcg agcgatttcg gatcagtggc gaagccgtgg ttttgcgcgg
      721 tgatcgacac tttgccggtc tcgaggtttt tcaccggttg attgccgccg cggtggccga
      781 acttgagctt gaaggtctca ccgcccaagg cgtgcgtgat catctggtga ccgagacaga
      841 tgccgaacgt cggataatgc ggcagcaggc cggtcaccgt tttgtgaacg tagtcgagcg
      901 cggcgggatc gccagggccg ttcgaaagga atacgccgtc ggggttttgc tcgcggatct
      961 gctcggcggt ggcggttgac gggaatacct gcacgtcgaa accgtggcgg atgagtttgc
     1021 ggaagatggt gtgcttcgcg ccgtagtcga aggcggccac gcggaatttc ttcgcgggaa
     1081 tgccgggagc gttgagcgtg gtgccgacag gaaggtaggg agcgttgtga ttcgcgagat
     1141 cgtcggcccg ccaaatgtag ggcacggcgc acgtgacgtc cttcacgtaa tcgctgccgg
     1201 ccatgtcatg ccaggcgcgg gcgcgggcga cggcgtcttc atcgctgatc ggcagggtcg
     1261 aaaggcagca tttcatcgcg ccgtcgacgc gaagtttttt cgtgagcgcg cgcgtgtcga
     1321 tttcgctgag gccggggatg ccgtgtttcg cgaggtagtc gtggagcgag atggtcgagc
     1381 gccagttgga gacgaccgga gagagttcgc gcacgatgag gccggcgcat ttcgggccgc
     1441 cggattcctc atcttccggg ctgagaccgt agttaccgat ctggacggcg gtcatcgtga
     1501 cgatctggcc gaagtaggac ggatcggtga tgatctcctg gtagccggtc atcgaggtgt
     1561 tgaaaacgca ctcgccggcg atggtggcgt cggcgccaaa ggcgaggccg cgataaacgc
     1621 tgccatcttc gagcgcgaga actccggttt tggacgggga cattaaagtc cgacgaaaag
     1681 gtctggagcg gggcgtgcca aggggaaaca tgcaccgatg gcgaggaagc cgggattttt
     1741 ggggaattcg ccggattggc gcagtcggga ccgagcggag tgcgggcaaa aaagccatcc
     1801 gcgaatggct tcgttcgaag aaagcgagtc ggcgcggcgc tcggatgttt agccgtgcgg
     1861 ggcggcttcg ccgcggcggg aacgaaaaaa cgcgaggagc agctggcgat tgtagatcgg
     1921 cacgagccca gcgttgattt gcacagggat gggccttttt agcggggccg tgatctccgt
     1981 ttccagttcg agcggcgctt cgggcgttcc ctggccgatc gcaagcaagg aacgaaagat
     2041 tcccgctggc atgaactggc tgagtcgctg gccgagcacg ccggcgcggt cgaggccgag
     2101 cagggtttca ccgcgtcggt ttgtgtcgat gatccgaccg gtggcggagt cgcacaaata
     2161 ggcggcatca gggaggtgct caaagaggca gcggtatttg tattcggatt gccgaaggtc
     2221 gtcttcggcg cgtcgcgcgg cgtcgcgaag ttttttctct tcgacggcct ggctgaccgc
     2281 ggcgccgagc cggccgagcc ggtcttttag gagatagtcg tcggcaccat tcttgatggc
     2341 ctcgacggcg atctcttcgc cgatcgaccc ggagacgacg ataaacggga tgtcgagctg
     2401 acgctgttgc acgatgcgca acgcttcggg cgcgctgaac gtgggcagcg cgtaatcgca
     2461 caaaatgata tccggctcga gcccgagctc gcggctgaaa tcctccgggt tctccacgcg
     2521 aatcacggtc gcggcggcgt agccggagcg tttcagctcc atgatcatca actcggcgtc
     2581 gagcgtttga tcttcaacca ggagaatgcg cagcggagcg acggaagcgg cggacggtgg
     2641 catgcgagaa gaaaatgagg cggcaatgat ccgaagaccg gaaaccgccg agatggagcc
     2701 acgcgaacgc gaagaaattg cgcggtaaat ttcgtgtaat tcttggttcg aactcttact
     2761 tcaaaccgaa cgcgtcattc gtgagaaaga aaacgtccgc gggattgacg ctgtatttgc
     2821 gtccattttt cacggtgacc tcgatcgcgc tgccgaccgc acgcacgtga gtgacgccgt
     2881 aaatgacgaa ttgtttgtcg ccgcgctgga ggacgagagc ggtttgctcg tcggcgagtt
     2941 ccttgaagac ctgctcgtgg ctgcgggcga gatcagccag ggatgcggcg gaagacgttt
     3001 gggcgtgagc ggacgtcgcg aagaagaaga aaagcgccac ggagcacgca gcgagaggga
     3061 gaatgttttt catgatcatg atgtgtgggc ggggtgccgc aggggcgcgg ttgcagctac
     3121 gtcgcggtca catagaccga tgcaccggct ccaggatcat gcgcgcggtt gggctttcga
     3181 ggtgaggctt tcgctgatcg tgagcagaaa aggggaacaa ggctttcgcg cggttgagcg
     3241 agcctgcacg attcaagagc cgatggattg cgcgtccggc gcggtgcaca acgcgccgtg
     3301 gctgattctc tcaaaaccat tttcgaccga tgagccaagg cacgcgttgt gcattgcact
     3361 gggcgtgcag ctcgtattcg aaagtccgtt acagatgctg gcggacgcgc cgactcacta
     3421 cgaagaggag ccggagtcga agcgagcgta tcagggtggt cggaatttta ccggtgcggg
     3481 tttaagagga aagccgtgga tttccccatg gcggcgatgt ccgggctgag cagggtgtcg
     3541 gccaagatca tgaagctttg ctgcgaaatt accatccacg ccgggagggc ttggccatga
     3601 gcggaacgct tgcggccggg cgacgtattc tggtcgtcga cgacgacgac atggtgcggg
     3661 attttctggt cggtcatctg ttgcgagcgc tgccgtcggc gcacgtgttg accgccaacg
     3721 atggcacgtc gggattggtg tcctatcggc gggagttgcc cgacctgatc gtcaccgaac
     3781 tcaacatgca tcagatgtcg ggggacgagc tcatcgagca catccgaaaa acggatacaa
     3841 cgacgccgat cctggtgctc acgggtgcgc cggagacgac gcccattccg ggtgcgacgg
     3901 aagtcgtgcc gaaaatccgc ctcggctatc tcgtgcaacg cgtcaccgac ctgctggcga
     3961 actcgccacg cgtcgaggag acgcgcggtt cggcgcggtg agcagttagc ggcgttcggc
     4021 cggtggctgg cttgcttact ggggcgtggc gct
//
LOCUS       DASPLT010001569         1079 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_223463, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001569 DASPLT010000000
VERSION     DASPLT010001569.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1079)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1079)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1079
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_223463"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..644
                     /locus_tag="VEA63_16010"
     CDS             <1..644
                     /locus_tag="VEA63_16010"
                     /inference="COORDINATES: protein motif:HMM:NF042701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SAM-dependent chlorinase/fluorinase"
                     /protein_id="HYD85573.1"
                     /translation="SVIDPGVGTARKSVVLRTKSGHFFVGPDNGTWTLVAEELGVTAV
                     REIDETKHRRAGSERSHTFHGRDVYVYTGAELAAGTTSFADIGPELAPRVVELAYEKA
                     RLEDGALIGTIPYLDFQFGNVWTNLGETWWARLAPKAGERFRVTIAQAGRTVYEGTMP
                     YARTFGDVAEGEPLLYLNSLMNVAVALNQGNFAKTHGISSGAEWSVRVERAAP"
     gene            641..>1079
                     /locus_tag="VEA63_16015"
     CDS             641..>1079
                     /locus_tag="VEA63_16015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD85574.1"
                     /translation="MRRATWALGAAVMWACAGCETGAGGRREDGAVSARFYLESTRDG
                     AGNATLPRSDVTISILPKPVITEFDVVSVEIAEVELGRCLKFQLTPAASRDLYRLTAS
                     RLGRRLVLFLNGQAVGARRIDQPIEDGALLIFVERPDEALPALV"
BASE COUNT          180 a          272 c          430 g          197 t
ORIGIN      
        1 tgtcggtgat cgatcctggt gtcggcacgg cacgaaagtc ggtcgtgctg cgaaccaaga
       61 gcgggcattt ctttgtcggg ccggacaacg ggacgtggac attggtggcg gaggaactgg
      121 gcgtgaccgc ggtgcgcgag atcgacgaga cgaagcatcg gcgcgcgggg tcggagcgat
      181 cgcacacgtt tcacggacgc gatgtgtatg tttacacggg cgcggagctg gcggctggca
      241 cgacgtcgtt tgcggacatc ggtccggagc tcgcgccgcg cgtggtggag cttgcgtatg
      301 agaaagcgcg gctcgaggac ggcgcgttaa tcggaacgat tccgtatttg gattttcaat
      361 tcggcaacgt gtggacgaat ctcggcgaga cgtggtgggc gcggctcgcg cccaaagccg
      421 gcgagcggtt ccgggtgacg atcgcgcagg cggggaggac cgtttacgaa ggcacgatgc
      481 cctatgcgcg gaccttcggc gacgtggcgg agggcgagcc gttgctctat ttgaacagct
      541 tgatgaatgt agcggtcgcg ttgaaccagg gaaactttgc caagacgcac ggaattagtt
      601 ccggtgcgga gtggagcgtg cgcgtggaaa gggccgcacc gtgaggcggg cgacgtgggc
      661 gctgggcgcg gcggtgatgt gggcgtgcgc gggttgtgag accggggcgg gaggccggcg
      721 cgaggacggg gcggtctcgg cgcgttttta cctcgagtcg acgcgcgatg gcgcgggcaa
      781 cgcgacgctg ccgcgaagcg atgtgacgat ttccattttg ccgaaaccgg tgatcacgga
      841 gttcgacgtg gtcagcgtgg agatcgcgga agtggagctc ggacgctgcc tgaagtttca
      901 acttacgccg gcggcgagcc gcgatcttta ccggttaacg gcatcgcggc tcgggcggcg
      961 gctcgtgttg tttttgaacg ggcaggcggt cggagcgcgg cggatcgatc aaccgattga
     1021 ggacggcgca ctgctgattt tcgtggagag gcctgacgag gcgctgccgg cgctggtgg
//
LOCUS       DASPLT010001570         1251 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_79685, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001570 DASPLT010000000
VERSION     DASPLT010001570.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1251)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1251)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1251
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_79685"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            134..>1251
                     /locus_tag="VEA63_16020"
     CDS             134..>1251
                     /locus_tag="VEA63_16020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015486 -
                     glycoside-pentoside-hexuronide:cation symporter activity
                     [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide (GPH):cation
                     symporter"
                     /protein_id="HYD85575.1"
                     /translation="MTQDKLPVREKLAYGCGDFASCLYWRTFMVYLPFFYTDVFGITA
                     AALGTMLLVSRIWDGVNDPIIGMFADRTETRFGKFRPYIIYGCVPFAIFGVLTFTTPD
                     LGMTGKLIWAYITYNGLMMLYTTVNIPYTAMLGVMTTTAAERTQLSSVKFVFAFSAGI
                     AVSATLLPLVDFLGGDNAQRGWQLAFVVYGLVAIGFFLVTGFGTRERIKPVPDENTSV
                     ARDLKYVFTNKAWLLLTATTLAWILFVGLRSSVSAHYFKYYIYNGTPDAPLSFLGTNF
                     TLTGLLSAFNTLGQAASVAGVLMTSFLASRFAKRPLFFVLFTISILCTASYYFLPPGQ
                     LGAIFAIEIFVNLSGAPLPVLLWAMYADTADYGEWRTGR"
BASE COUNT          198 a          433 c          323 g          297 t
ORIGIN      
        1 ggtcacattt ccgccgcaac caatctcatt tttgcggccg atttcgggct ggcattgtga
       61 cccccagatt attgtctcgc cgtgccccgg cggctctccg cctcgttcac ttcgactgcc
      121 agcgttcccc atcatgaccc aagacaagct tcccgttcgc gagaaactgg cctacggctg
      181 cggcgatttt gcctcctgcc tttattggcg gaccttcatg gtctacctgc cgtttttcta
      241 caccgacgtt ttcggtatca ccgccgcggc gctcggcacc atgctgttgg tcagccgcat
      301 ctgggacggc gtcaacgacc cgatcatcgg gatgttcgcc gatcgcaccg aaacgcgttt
      361 cggcaaattc cgcccttaca tcatttatgg ctgcgtgccg ttcgcgattt tcggcgtgct
      421 cacgttcacg acgcccgacc tcgggatgac gggcaaactc atctgggctt acatcactta
      481 caacggcctc atgatgctct acacgacggt gaacatcccc tacaccgcga tgctcggcgt
      541 gatgaccacg acggccgcgg agcgcacgca gttgtcgtcg gtgaagttcg tcttcgcgtt
      601 ctccgccggc atcgccgtct ccgccacgct gctgccgctc gtcgatttcc tcggcggcga
      661 caacgcgcag cgcggctggc agctcgcctt tgtcgtctac ggcctcgtcg cgatcggctt
      721 ttttctcgtc acgggtttcg ggacacgcga acgaatcaaa cccgttccgg acgaaaacac
      781 gtccgtcgcg cgcgacctca aatacgtttt cacgaacaaa gcctggctcc tgctcaccgc
      841 gacgacgctc gcgtggatct tgttcgtcgg ccttcgcagc tccgtctccg cgcactattt
      901 caagtattac atctacaacg gcacgcccga cgctccgctg tcgtttctcg gcaccaactt
      961 tacactcacg ggactgctct ccgcgttcaa cacgctcggg caagccgcgt cggtcgccgg
     1021 cgtgttgatg acgagtttcc tcgcgagccg attcgcgaaa cgaccgctct tcttcgtgct
     1081 gttcacgatt tcgattctct gcaccgcgtc ctactacttc ctgccacccg gccaactcgg
     1141 cgcgattttc gccatcgaga tctttgtgaa tctctccggt gcgccgctgc ctgtgctgct
     1201 ttgggcgatg tatgccgaca ccgccgatta cggcgaatgg cgcaccggtc g
//
LOCUS       DASPLT010001571         1417 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_223477, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001571 DASPLT010000000
VERSION     DASPLT010001571.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1417)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1417)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1417
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_223477"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..186
                     /locus_tag="VEA63_16025"
     CDS             <1..186
                     /locus_tag="VEA63_16025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-monophosphate kinase"
                     /protein_id="HYD85576.1"
                     /translation="GEDYELVFAVAAKTDHARFERTWQRAFPRTPLSRIGHFVPSRQL
                     PPSAVNLADYRGYEHLR"
     gene            195..629
                     /gene="tsaE"
                     /locus_tag="VEA63_16030"
     CDS             195..629
                     /gene="tsaE"
                     /locus_tag="VEA63_16030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HYD85577.1"
                     /translation="MDIVEKLHAGVITRSPAETQQFATEFAAELPPDVALALHGNLGV
                     GKTTFVQGLARGFGVTEPVTSPTFNIYTVHRGPRRTLVHLDAYRLESAQQIENLLLED
                     FLISPWCLAVEWPEKIAGFLPPTTLHLDLAIGPDETHSLRLR"
     gene            complement(682..>1417)
                     /locus_tag="VEA63_16035"
     CDS             complement(682..>1417)
                     /locus_tag="VEA63_16035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009512381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD85578.1"
                     /translation="FTIFKKGSKGVSYVCAVKRKFRVPGQTFSDTIGALIAFIEANPM
                     VRASELPAKFLGITANVSATKPAEAATDAGAPETVRPAATQESALSIDERAKISRMQG
                     DLLWLVREGYVTEFIDGRLFAPPPMVEARKKEVEAEEHDPENFPDAPPAEKSAAAATT
                     APAAEPNVSTTPDAQSETGTPVEALPSDPVTQEASPIPNLPDPVVPAAPEASEASSAG
                     DGTDRTNGTNGTAAAGEEKPTPSQTS"
BASE COUNT          238 a          518 c          398 g          263 t
ORIGIN      
        1 ggcgaagact acgaactcgt tttcgccgta gccgctaaga ccgaccacgc tcgcttcgaa
       61 cgcacgtggc aacgcgcttt cccgcgcacg ccgctcagcc gtatcggcca tttcgttcct
      121 tcgcgccaac ttccgccttc cgccgtgaac ctcgccgact atcgcggcta cgaacacctc
      181 cgctgacgct ccgcatggac atcgttgaaa aactccacgc cggcgtaatc acccgctccc
      241 ccgccgaaac ccagcagttc gccaccgagt tcgccgccga gctgccgccc gatgtggcgc
      301 tcgcgttgca tggcaatctc ggcgtcggca aaaccacatt cgtccaaggc ctcgcacgcg
      361 gctttggcgt aaccgaaccg gtcacgagcc cgaccttcaa tatctacacg gtgcatcgcg
      421 gcccgcgacg cacgctcgtg caccttgacg cctatcggct cgagagcgca caacaaatcg
      481 agaatctact gctcgaggat ttcctcatct cgccgtggtg cctggccgtc gaatggcctg
      541 aaaaaatcgc aggcttcctg cccccgacga cgctgcacct cgacctcgcg atcggccccg
      601 acgaaacgca ctccctgcgc ctccgctaaa acagcagcgg cagacccgca ggtctgccgc
      661 tcctcacgaa aagaagtcgg attagctggt ctgcgacggc gtcggctttt cttcgcccgc
      721 tgccgcagtc ccattcgtcc cattcgtcct atcagtccca tctccagccg acgacgcctc
      781 actagcctcc ggcgccgcag gcaccaccgg atccggcaga ttcggaatcg gactcgcttc
      841 ctgcgtcacc ggatcgctgg gcaacgcttc aaccggcgtt ccggtttcgc tttgcgcgtc
      901 cggcgtcgtt gaaacgttcg gctccgccgc gggcgccgtc gtcgcggctg ccgcggattt
      961 ctccgccggc ggcgcgtcag gaaaattctc cggatcgtgc tcctcggctt cgacttcctt
     1021 cttgcgcgcc tcgaccatcg gcggcggcgc gaacaggcgg ccgtcgatga actcggtcac
     1081 atagccttcc cgcacgagcc aaagcaggtc gccctgcatg cgggaaatct tggcgcgttc
     1141 gtcgatcgag agcgccgatt cctgggtcgc ggcgggacgc accgtctccg gcgcgccggc
     1201 atcggtcgcg gcttcggccg gcttggtggc actgacgttc gccgtgatgc cgaggaactt
     1261 ggccggcaat tcgctcgcgc gcaccatcgg gttcgcctcg atgaacgcga tcagcgcgcc
     1321 gattgtatcc gagaacgtct gacccgggac gcggaacttg cgcttcacgg cgcagacgta
     1381 cgaaacgccc ttcgagcctt tcttgaagat cgtgaag
//
LOCUS       DASPLT010001572         1295 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_223500, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001572 DASPLT010000000
VERSION     DASPLT010001572.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1295)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1295)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1295
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_223500"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..511)
                     /locus_tag="VEA63_16040"
     CDS             complement(<1..511)
                     /locus_tag="VEA63_16040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD85579.1"
                     /translation="MNFARFLAALTGLAAVLLPRVHAEDNAWPVRVAQTDDAGNVTSW
                     EAAGPLFFQKPAANAGTVAGFRPFFAQWKTAGGEVAETNVLYPLFAYRTDGETYRWSV
                     LQLINRSGDRAERKAELLPALSYDTFDVWPFWFSRDTRDPTTSYRALFPIHGEILNRL
                     GYDRIRFTLF"
     gene            820..>1295
                     /locus_tag="VEA63_16045"
     CDS             820..>1295
                     /locus_tag="VEA63_16045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmD family RNA methyltransferase"
                     /protein_id="HYD85580.1"
                     /translation="MDAICAGRGAAMRISGGGARGITLKVPKGDAVRPATDGMRQAVF
                     SSLGARVDGARFLDLFAGSGAYGLEAVSRGAAGGVFVEKNAKAAACVRENIAAVCRSL
                     GRDAAGLSVLQADATRVPVSEGGDDVPNLVFVDPPYEIIAEIAKPLFVRLGEVLAP"
BASE COUNT          243 a          326 c          496 g          230 t
ORIGIN      
        1 ggaaaagtgt gaagcggatg cgatcgtaac cgaggcggtt caggatttcg ccatgaatcg
       61 gaaacagcgc gcgataggaa gtcgtgggat cacgggtgtc gcgcgaaaac caaaacggcc
      121 agacgtcgaa tgtgtcgtag ctgagcgccg gcaggagttc ggctttccgt tcggcacgat
      181 cgccggagcg gttgatcaac tggagcacgc tccagcgata ggtctcgccg tcggtgcggt
      241 aggcaaagag cggatacagg acgttggtct cggcgacttc accgccggcg gttttccatt
      301 gcgcgaagaa cgggcgaaag cccgcgacgg tgccggcgtt ggcggcgggt ttttgaaaga
      361 aaagggggcc ggcagcttcc cacgaggtga cgttgccggc gtcgtcggtc tgcgccacgc
      421 gaaccggcca ggcgttgtcc tccgcgtgaa cgcgcggcag caacacggcg gcgaggccgg
      481 tcagggcagc gaggaagcgg gcgaagttca tgatgtagat tagcatccgg gcatgcgact
      541 gcccatcggg tccgcggagg agtcggccaa ccgggaccag cgatgctaag agttgagagg
      601 taggtgtttt ccggaacggc gcaaatgcta tgttgaaccg ctagcgaaga ccggtgttcc
      661 gccggatgca ggcgcagagg catggcaaat cgccaattcg tcgccggcga gaacgtgaac
      721 cacctgcttc ggaacggccg gtgcaatgaa cgatttcccg ttgaagtggt tggacagcgg
      781 aggtgtcatc gcgacacgag ctatgaattt tgtttcgcga tggacgcaat ttgcgcgggg
      841 aggggagcgg cgatgcgcat aagcggcgga ggtgcgcgcg gcatcacgct caaggtgccg
      901 aagggcgacg cggttcgccc tgcgacggac gggatgcgac aggcggtgtt ctcgagtttg
      961 ggagcgcggg tggacggcgc gcggtttctc gacctgttcg ccggcagcgg tgcgtatggg
     1021 ttggaagcgg tgagtcgtgg cgcggcgggt ggcgtgttcg tggaaaagaa cgcgaaggcg
     1081 gcggcgtgcg tgcgcgaaaa catcgcggcg gtgtgtcgca gtctggggcg agacgcggcg
     1141 gggttgagcg tgctccaagc ggatgcgacg cgtgtgccgg tttccgaggg gggcgacgac
     1201 gttccgaatt tggtgtttgt cgatccgcct tacgagatca tcgccgagat cgcgaagccg
     1261 ttgtttgtgc gattgggtga agtgctcgcg ccgaa
//
LOCUS       DASPLT010001573         1286 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_79717, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001573 DASPLT010000000
VERSION     DASPLT010001573.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1286)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1286)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1286
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_79717"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1080)
                     /locus_tag="VEA63_16050"
     CDS             complement(<1..1080)
                     /locus_tag="VEA63_16050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD85581.1"
                     /translation="MKSSKQKLLALLLGCVVLGSAGLAAESQTSAEVTLPTPPPRHPR
                     LYVSPADVAGLGQRLRDPVLRSNVARLEKLAERSAQYRVEWQALHYLVEPDETRGRAI
                     IDETLQLLKASDLPDYRLTQRPDQSGGCRVTGRMMVTGALVYDWLYPLFSDVDKKAYI
                     AELVRLAKTQECGYPPTRGGSITGHGSEAMILRDMLSAGIAIYDEDPEMYRLAATRVL
                     GQHVRARNWLYPGHACHQGDSYGPYRFQWDLFALFIFDRMGAGNVYNPEQRFIPYEWV
                     YTTRPDGQRLRSGDTFKDSKPRGTPWDESLSTLLTASYYGDGLLLGHFLKHGAGADDE
                     AIFEFLWRDTALEPKPSDTLPLSKYF"
     gene            complement(1095..>1286)
                     /locus_tag="VEA63_16055"
     CDS             complement(1095..>1286)
                     /locus_tag="VEA63_16055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD85582.1"
                     /translation="VLAEARGNFAYVVQDLDLNAEWRQRYLSVASGMGEGKSLYIVER
                     RPDTYQELSDTAIPAAERR"
BASE COUNT          249 a          424 c          388 g          225 t
ORIGIN      
        1 gaaatatttc gagagcggca acgtgtccga cggcttcggc tcgagcgcgg tatcgcgcca
       61 gagaaattcg aagatcgcct catcatccgc gcccgcgccg tgcttcagaa aatggccgag
      121 cagcaggccg tcgccgtagt agctcgcggt gagcagcgtg ctgagcgact cgtcccacgg
      181 cgtgccacgc ggcttgctgt ccttgaacgt atcgccgctg cggagacgct ggccgtcggg
      241 ccgcgtggta taaacccact cgtaagggat gaagcgctgc tcgggattgt agacattgcc
      301 tgcgcccatc cggtcgaaaa tgaacaacgc gaacagatcc cattgaaaac ggtaaggacc
      361 gtaggaatcg ccctggtgac acgcgtggcc cggatagagc cagttgcgcg cccgcacgtg
      421 ctgacccagc acgcgcgtcg cggcgagccg atacatctcg ggatcttcat cgtagatcgc
      481 gatgcccgcc gaaagcatgt cgcgcaggat catcgcttcc gaaccgtgtc cggtgatcga
      541 gccgccgcgc gtcggcggat agccgcactc ctgcgttttc gcgagccgaa cgagctccgc
      601 gatgtaggcc ttcttgtcca cgtcgctaaa aagcggatac aaccaatcgt agaccagcgc
      661 gcccgtgacc atcatccgcc cggtcacccg acagccgccg ctctggtcgg gccgttgcgt
      721 caggcgatag tcgggcagat cggacgcctt caacaactgc agcgtctcgt cgatgatcgc
      781 acgaccccgc gtctcgtccg gctcgacgag atagtggagc gcctgccact cgacgcgata
      841 ttgcgccgag cgctccgcga gcttctccaa ccgcgccacg ttcgaacgca gcacgggatc
      901 gcgcaggcgt tgtccaagtc cggccacgtc cgccgggctc acgtaaagcc gcggatgccg
      961 cggcggcgga gtcggcaacg tgacttccgc actcgtctgc gattccgccg ccaagcctgc
     1021 agacccgaga acgacgcagc ccaataacag agcgagcagt ttttgtttcg aagatttcat
     1081 gaagacgttt cggttcatcg ccgttcggcg gcagggatgg cagtgtcgga caattcctga
     1141 tacgtgtcgg gccgccgctc gacgatgtag aggctcttcc cctcgcccat gccgctcgcg
     1201 acggagagat accgctgacg ccactccgca ttcagatcca gatcctggac gacataagca
     1261 aagttgccgc gcgcttctgc cagcac
//
LOCUS       DASPLT010001574         2603 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_243416, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001574 DASPLT010000000
VERSION     DASPLT010001574.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 2603)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2603)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2603
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_243416"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..927
                     /locus_tag="VEA63_16060"
     CDS             <1..927
                     /locus_tag="VEA63_16060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD85583.1"
                     /translation="DAGLLRVLPGSNEPGYIEIAGDIMDVLASRVTPAHVFSKKFSDK
                     AYSIGLGGLGDRRDDYFNLMGEMITIGGTMVWLPCDGNDRPDFLIPHRDSGQVLLHTG
                     FNASLSGAFNEQMEFVSSRPEGATMSEVYRLIFDHAKAHRPDYKGVVGLAMRAEMSAV
                     YGAGVVRAPVAPNAPANGKLITDESNFASWFEFDREPRHRNVTGLVAGCGIDLTANLD
                     GLNHRCLKRTFYLNPANAGASNMTLHNHVVMFDPLPMPAAGFTLESEISAVVEKGEFR
                     DMRHMLDMSTIKRAVIGVIYAQDFRPDPECVR"
     gene            complement(1291..>2603)
                     /locus_tag="VEA63_16065"
     CDS             complement(1291..>2603)
                     /locus_tag="VEA63_16065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD85584.1"
                     /translation="SASGTLTINPASQSISFANPGDMIIGESRTLSASASSGLSVAFS
                     VIEGPATLSGNTLTATGVGAVTVRASQSGSGNYTTAPNVDRSFSVTVGAPTVTTPEQI
                     PAPPAGGSAELEVVNSNPNVTYQWQRNGSNLPNATGPSLTLDDVQPPTAGLYTYTATV
                     PGGSGGTSEPVIVGLSTEEKVIGEGEQVLADVQHPNGNVYDQVLLEGNGASITASPNK
                     VTRISFVDLTSDIVQVEFSGAGTLSVVMDDASSPTPAENYNQPGVEYVRGHVGLVITG
                     ANETSNLSVISVGPITAVNQTLFKEGVAYDGVADIAFVAIQSADGKFGSIRTGNVHYF
                     ASQGFTGVYAPGVEFAGPVNIGELTAFDSATPVLVFGAIELVKITGGNLEQANDAPVS
                     VRGIEEVRFVDGTTSQGDTIPAQVNAGVLVEDGVDITDLIVVNP"
BASE COUNT          468 a          890 c          725 g          520 t
ORIGIN      
        1 gacgccggcc tgcttcgcgt tttgcccggc tccaatgaac ccggctatat cgagatcgcc
       61 ggcgatatta tggacgttct cgcttcgcgc gtgacacctg cgcacgtgtt ctcgaaaaag
      121 ttttccgaca aagcctactc catcggtctc ggcggcctcg gcgatcgtcg cgacgactac
      181 ttcaatctca tgggcgaaat gatcaccatc ggcggcacga tggtctggct cccctgcgac
      241 ggaaacgacc gtcccgactt cctcatccca catcgcgata gcggccaggt tctcctgcac
      301 acgggcttca acgcttcgct ttcaggcgcc ttcaacgagc aaatggaatt cgtcagctcc
      361 cgcccggaag gtgcgacgat gagcgaggtt tatcgtttga ttttcgatca cgcgaaagcc
      421 caccgccccg actacaaggg cgttgtcggt ctcgcgatgc gcgcggaaat gtccgcggtt
      481 tacggcgccg gcgtcgtgcg cgcgcctgtc gcgccgaacg ctcctgccaa cggcaagctc
      541 atcaccgacg agtcgaattt cgcctcctgg ttcgagttcg accgcgagcc gcgtcaccgc
      601 aacgtcaccg gcttggtcgc gggctgcggc atcgatctca ccgcaaattt ggacggcttg
      661 aaccatcgct gcctcaagcg gacgttctat ctcaatcccg cgaacgccgg cgcgtccaac
      721 atgacgctgc acaatcacgt cgtgatgttc gatccgctcc cgatgcctgc cgccggcttc
      781 acgctcgaaa gcgaaatttc cgccgtcgtc gaaaaaggcg agttccgcga catgcgccac
      841 atgctcgaca tgagcacgat caagcgcgcc gtgatcggcg tcatctacgc acaggatttt
      901 cgccccgatc ccgagtgcgt ccgctagagc gtttccaaag ctgtagccgt tgctgaagcg
      961 gaggcaggag cctgcttgca ggcgatttca ggcgtcgccc gcgatcccgc ggcatcgcct
     1021 gcaagcaggc tcctattatg gtgtcccaca agaaacctga ataatcgatg gcgcggcagc
     1081 gccggtaggg acgcttctcc gaggcgtcct cgcacactct gcgacaccat actacactac
     1141 agcggttcaa aaaattctag agccgaactt ttggccgcaa agaggcgcaa agaggcgcaa
     1201 gagcagaaaa gtcctcgaag tcatttttgc gcctcctgtg tctttttgcg gccatggtct
     1261 tcattgatct attcacatct ctgagcggag ctacgggttc acgacgatca ggtcggtgat
     1321 gtcgacgccg tcttcgacaa gcacgccggc gttcacctgc gcggggatcg tgtcgccttg
     1381 cgaggtcgtt ccgtccacga aacgcacctc ttcgattcct cgcaccgaca ccggcgcatc
     1441 attcgcctgc tccaggttgc cgcccgtgat cttcaccagc tcgatcgcac cgaacaccag
     1501 caccggcgtg gccgagtcga acgccgtcaa ctcgccgatg ttcaccggtc ccgcgaactc
     1561 cacgcccggc gcgtagacac ccgtgaaacc ctggctggcg aagtagtgca cgttgcccgt
     1621 gcgaatgctg ccgaactttc cgtcggcgct ctggatcgcc acgaacgcga tgtccgccac
     1681 gccgtcgtaa gccacgcctt ccttgaacag cgtctggttc accgccgtga tcggccccac
     1741 gctgatcacc gacagattgc tcgtctcatt cgctcccgtg atcacgagcc cgacgtggcc
     1801 tcgcacatac tcgacgcccg gctgattgta attctccgcg ggagtcgggc tcgaggcatc
     1861 gtccatcacc accgacaagg tgcccgcgcc gctgaattcc acttgcacga tgtcgctggt
     1921 caaatcgaca aacgagatgc gcgtgacctt gttcggactc gccgtgatcg acgcaccgtt
     1981 tccttcgagc aacacctgat cgtaaacatt gccgttggga tgttggacgt cggcgagcac
     2041 ctgctcgccc tcgccgatca ctttctcttc cgtcgacaat cccacgatca ccggctcgct
     2101 cgtgcctccg ctgccgccgg gcaccgtcgc agtgtaagtg tagagacccg ccgtcggcgg
     2161 ctgcacgtcg tcgagcgtga gcgaagggcc ggtcgcgttg ggcaggttgc tgccgttgcg
     2221 ctgccactga taagtcacgt tcgggttgct gttcaccacc tccaactccg ccgacccgcc
     2281 cgccggcggc gccgggatct gctcgggcgt tgtcactgtc ggcgcaccca ccgtcacgct
     2341 gaagctgcgg tcgacgtttg gcgctgtagt gtagtttcca ctaccggact gcgatgcgcg
     2401 caccgtcacc gctcccacgc ccgtcgccgt cagcgtgttc cccgacaagg tcgccggtcc
     2461 ttcgatcacg gagaacgcca ccgacaaccc cgaactcgcc gacgccgaca acgtccggct
     2521 ctcgccgata atcatatcgc cgggattcgc gaaggagatc gattgcgacg ccgggttgat
     2581 tgtcagcgtg ccgctcgcgc tgc
//
LOCUS       DASPLT010001575         1383 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_223538, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001575 DASPLT010000000
VERSION     DASPLT010001575.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1383)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1383)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1383
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_223538"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1031
                     /locus_tag="VEA63_16070"
     CDS             <1..1031
                     /locus_tag="VEA63_16070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD85585.1"
                     /translation="SVTIGILGMVLVHVLLFWIGPHLLESGSAQSVLRPHSSDRSFDM
                     EIAPDTFAEEEEAPPSNFVEANPDAPDNVPDQTNNFAAQNQQVAQEKPTPDGESDRPA
                     MEGRTDIQSTQIVDGSLTQPIESMPEPPPAEEQMERPNEATPQREQIPLSGVEKSQGE
                     NESTFGSNIAKFEQGSPDVKEYVEGQKDAPLIQGATGSQPRIDPKRPRPRPQVVKQQQ
                     VRPAIFQENKFGTKNIGLAAWDAKWSNYGQYLQKLIDTVQIQWERILIESRVYPVNGT
                     SVKVVFVLNAEGAVARIVSVDGTAGNQAEKACASAITSRSPYGPWTEDMIAILGQEQE
                     LTFNFFYQ"
     gene            1028..>1383
                     /locus_tag="VEA63_16075"
     CDS             1028..>1383
                     /locus_tag="VEA63_16075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HYD85586.1"
                     /translation="MSAAAGSFWSAAPLGRGVEILAGDANGVAALAKPAGVLSHPNEA
                     KDQPRSLIVAHYDAAEECYQWRVEGEGEPQRAWLLNRLDSATSGVILLANDAELAREI
                     RAQFKRKQVTKIYQALV"
BASE COUNT          299 a          376 c          486 g          222 t
ORIGIN      
        1 ggtcggtgac gatcgggatt ttgggcatgg tgctcgtgca cgtgctgctg ttttggatcg
       61 gaccgcattt attggagagc ggctccgcgc agagcgtgtt gcggccacat tcgtcggatc
      121 gcagtttcga catggaaatc gcgccggaca ccttcgccga ggaggaggaa gcgccgccgt
      181 cgaatttcgt ggaagcgaat cccgacgcgc ccgacaacgt cccggatcag acgaacaatt
      241 tcgcggcgca gaatcagcaa gtggcgcagg agaaacccac gcctgacggc gaaagcgacc
      301 ggccggcgat ggaaggccgc acggacattc aatcgaccca gatcgtcgac ggctcgttga
      361 cgcagccgat cgagtcgatg ccggagccgc cgccggcgga ggagcagatg gaacggccga
      421 acgaggcgac accgcagcgc gagcagattc cgttgagcgg cgtcgagaaa tcgcagggcg
      481 aaaacgaaag cacgttcggc agcaatatcg cgaaattcga gcagggttcg ccggacgtaa
      541 aagaatatgt cgaaggccaa aaagacgcgc cgctcatcca aggcgcgacc ggctcacaac
      601 cgcgcatcga tccgaagcgt ccacgcccgc ggccgcaggt cgtgaaacag cagcaggtgc
      661 ggccggcaat ttttcaggaa aacaagttcg gcacgaaaaa tatcgggctc gcggcgtggg
      721 atgcgaagtg gagcaactac ggacagtatc tccagaagct gatcgacacg gtgcagatcc
      781 agtgggagcg cattttgatc gagagccgcg tgtatccggt caacggcacg tccgtgaaag
      841 tcgttttcgt gctgaacgcg gaaggcgcgg tcgcgcgcat cgtgagcgtc gacggcaccg
      901 cgggaaatca ggcggagaaa gcctgcgcga gcgcgatcac gtcgcgctcg ccgtatgggc
      961 cgtggacgga ggacatgatc gcgatcctcg ggcaggagca ggagctgacg tttaatttct
     1021 tttatcagtg agcgccgcgg cgggcagttt ttggtccgcg gcaccgttgg gacgcggggt
     1081 ggaaattttg gcgggcgacg cgaatggcgt ggcggcgctg gcgaaaccgg cgggcgtgtt
     1141 gtcgcatccg aacgaggcga aggatcagcc gcgttcgctc atcgtcgcgc actacgatgc
     1201 ggcggaggag tgctaccagt ggcgagtgga gggagagggt gaaccgcagc gcgcctggtt
     1261 gttgaaccgg ctcgattcgg cgacgtcggg cgtgatcctg ctcgcgaacg atgcggaact
     1321 cgcgcgggag attcgcgcgc agttcaaacg aaagcaggtg acgaaaattt atcaggcgct
     1381 cgt
//
LOCUS       DASPLT010001576         2008 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_19421, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001576 DASPLT010000000
VERSION     DASPLT010001576.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 2008)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2008)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2008
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_19421"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            191..388
                     /locus_tag="VEA63_16080"
     CDS             191..388
                     /locus_tag="VEA63_16080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007364256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YezD family protein"
                     /protein_id="HYD85587.1"
                     /translation="MPATGLTDTPHAANGATPDWIALVREKVEGLRYGVVQLVVHDGR
                     VTQIERTEKTRLPATREQTAE"
     gene            510..1895
                     /locus_tag="VEA63_16085"
     CDS             510..1895
                     /locus_tag="VEA63_16085"
                     /inference="COORDINATES: protein motif:HMM:NF019042.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HYD85588.1"
                     /translation="MNLFRSAPRAALILAFALPGLFAAEAQSRDDDIQALREQIRLLD
                     QKLRVLERNQELKDEAATAAAKAAPKVTINDKGFTLASGDSANSIRLRGLVQLDARLF
                     FGDDGAARDAFVLRRARLISEGVLAKNFGFQFVTEFGGSSVSILDANLTVALSKTTQL
                     KFGKFKAPVGLEQLQSDTWTFFNERSIVTNLVPNRDLGIQISGKLLGDTVEYALGVFN
                     GLPDGGSSNNTDFDNDKDVVARVFATPFKDETESPLSGLSFGVAGSIGRQESASGRTG
                     GFRTDGQQTFFSYNAATVADGQTWRVSPQVDYRHGPFGFIGEYVLSTINVRPSATGAK
                     TELQNEAWQLAFGYVLTGENSSYSGVVPKSDFNLSAGTWGAFEVVARVAQLDVDDAAF
                     PVFASPATSANEASSFGVGLNWYLSKAAMLKLDYYQTDFGFNAVAPAVSNTPFLRQDE
                     QTFITRFQIGF"
BASE COUNT          375 a          689 c          561 g          383 t
ORIGIN      
        1 aaacttcgtc gcgacaacct gccgctgccg ttttccgccg aggccgaagc cgcccacggc
       61 gttccctccc gtattcccgc cagcttcgcc cgcaagctcg ccaagcgcgg cacgccgcgc
      121 cggctcaccg aggccgatgg cgtgctccat caaattctcg gcgattacac catttagtcc
      181 cctgtctgtc atgcccgcca ccggccttac cgatactcct cacgccgcca atggtgcgac
      241 gcccgactgg atcgcgctcg ttcgcgagaa ggtcgaaggc cttcgctacg gggtcgtgca
      301 actcgtcgtc cacgacggtc gcgtgaccca gatcgagcga actgaaaaga cgcggctgcc
      361 cgccacgcgc gagcaaaccg cggaatagcc cgcacacgat ttccctgcgt cgaccgttac
      421 ccgcggaggc gttccaccaa gcaagtccaa ctccccggga ttcgaccagg caactggagt
      481 gctcctgaga accatcaact cagtcattca tgaacctctt ccgttcggcc ccacgggccg
      541 ctcttatcct cgccttcgct cttcccggct tgttcgccgc ggaagcgcaa tcgcgcgacg
      601 acgatatcca agcactccgg gaacagatcc ggttgcttga ccaaaaactc cgcgtcctgg
      661 agcgaaacca ggagctcaag gacgaagccg ccaccgctgc tgcgaaggcc gcgcccaaag
      721 tcaccatcaa cgacaagggc ttcacgctcg cgtcgggcga cagcgcgaac tcgattcgcc
      781 tccgcggtct cgtgcagctc gatgcgcgtc tcttcttcgg cgacgacggc gcggcccgcg
      841 atgccttcgt gctccgtcgc gcgcggctca tttccgaagg cgtgctcgcg aaaaatttcg
      901 gattccagtt cgtcaccgag ttcggtggca gcagcgtcag catcctcgac gcgaacctga
      961 cggtcgcgct ctcgaaaacg acgcagctga agttcggcaa gttcaaggcc cccgtcggcc
     1021 tcgagcagct tcagtcggac acgtggacgt ttttcaacga gcgctcgatc gtcacgaatc
     1081 tcgtgccgaa ccgcgatctg ggcatccaga tctccggcaa gctgctcggt gacacggtgg
     1141 aatatgcgct cggcgtgttc aacggactgc cggacggcgg aagctcgaac aacacggact
     1201 tcgacaacga caaggacgtc gttgcgcgcg tgttcgccac gccgttcaaa gacgagaccg
     1261 agtcgccgtt gagcggtttg tcgttcggtg tcgccggcag catcggccgg caggagagcg
     1321 cgtcgggccg cacgggcggc ttccgcaccg acggccagca gactttcttc agttacaacg
     1381 cggccaccgt ggccgacggt cagacgtggc gcgtttcgcc gcaggtcgac tatcgtcacg
     1441 gtcccttcgg cttcatcggc gaatatgttt tgtcgacgat caacgtccgc ccgagcgcga
     1501 ccggtgcgaa aaccgagctg cagaacgaag catggcaact cgccttcggt tatgtgctga
     1561 ccggcgaaaa ctcgtcctac tccggcgttg tcccgaagag cgatttcaat ctttccgcgg
     1621 gcacgtgggg cgcgttcgaa gtcgtcgctc gcgtcgcgca actcgacgtc gacgatgcgg
     1681 cctttccggt tttcgcttcg cccgccacgt ctgcaaacga agccagttcg ttcggcgtgg
     1741 gcctgaactg gtatctcagc aaagccgcga tgctgaagct ggactattac cagacggact
     1801 tcggcttcaa cgccgtcgcg cccgcggttt ccaacacgcc gttccttcgt caggacgagc
     1861 agacgttcat cacccgcttc cagatcggtt tctaatccca ttttcagact catcgttatc
     1921 atgaaaacga aatactttct cctctcgctc ggtgtcctcg cgctcgcgac gatcgcctcc
     1981 gccaaaaaaa tcgagctgct caacgtct
//
LOCUS       DASPLT010001577         1159 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_183612, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001577 DASPLT010000000
VERSION     DASPLT010001577.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1159)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1159)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1159
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_183612"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(183..>1159)
                     /locus_tag="VEA63_16090"
     CDS             complement(183..>1159)
                     /locus_tag="VEA63_16090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD85589.1"
                     /translation="SPALQTRADEFEAAAARLRTRIAEVIDRVTVHDTTPPFVPFALE
                     LPSLQGKNEGPHERLTATRFGNYWNLFAPSFLELRFSTPADASLPNRWIFNYAETHGG
                     LWAGLPRFYTGLDAAYAIGYIGELLDRSAEDRRARPQALAALHTYFLHAASRNSHSIP
                     EVAGLFPHRLDRAAYEQLVREAPWHFGMYDPERYLQGHASFTEPLGSGAGAALTMIRN
                     ALMAETRNELGLPDGGLILLPTVPADWFAEGKEIVLENFPTAYGVLSVRVRSLVASEN
                     KIAMEYRFEPFDEDFAPGRFRVRFAPAGKEMREVSFDPLQAGSLEVNW"
BASE COUNT          227 a          332 c          385 g          215 t
ORIGIN      
        1 ggaggcgcga tgggagttgt gacgcagctc gcgatcggct ggacggtgca gaacgtgtcg
       61 ttcacgccgg tgttcggcgt gtgctccatc gtgcacctcg tggcgttcgc tttggtgtgc
      121 ctgctgatcg gcgaactcgg ccggatccgc gaagtgccgg tgcgttgacc ggcaatcgac
      181 tcctaccaat tcacttcgag gcttccggct tgcagcgggt cgaacgagac ttcgcgcatt
      241 tctttgccgg ccggcgcaaa acgaacgcgg aaacggcccg gcgcgaaatc ttcatcgaag
      301 ggctcgaagc gatactccat cgcgatcttg ttttcgctgg cgacgagtga acgcacgcgc
      361 acggaaagca cgccgtaagc ggtggggaag ttttccagca cgatctcttt tccctcagca
      421 aaccagtcgg ccgggacagt tggcagcagg atcaatccgc catcgggcaa accgagttcg
      481 ttccgcgtct ccgccatcag ggcgttgcga atcatcgtga gcgctgcgcc agcgccggag
      541 ccgagtggct cggtgaacga ggcgtggcct tgaaggtagc gttcaggatc atacatcccg
      601 aaatgccacg gtgcttcacg gacgagttgc tcgtaagccg cgcgatcgag ccgatgaggg
      661 aaaagtcccg cgacctccgg aatcgagtga ctgttgcggc tcgccgcatg caggaaataa
      721 gtatgcagcg ccgccaatgc ttgtgggcgc gcgcgacgat cctcggccga acgatcgaga
      781 agctcgccga tgtagccgat ggcataagca gcatcgagcc cggtgtagaa tcgcggcagt
      841 ccagcccaaa gccctccgtg agtttccgca tagttgaaga tccagcgatt cgggagcgaa
      901 gcgtcggctg gcgtgctgaa acgcagttcg aggaacgaag gggcgaagag gttccagtag
      961 ttgccgaaac gagtcgcggt cagccgttcg tgcggacctt cgtttttccc ctggagagaa
     1021 ggcagctcga gcgcgaatgg gacgaaaggc ggcgtggtgt cgtgcaccgt cacccggtcg
     1081 atgacctcgg cgattcgcgt gcgcagacga gcagcggcag cttcgaattc gtcagcgcgt
     1141 gtttgcagcg caggcgaac
//
LOCUS       DASPLT010001578         1641 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_203950, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001578 DASPLT010000000
VERSION     DASPLT010001578.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1641)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1641)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1641
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_203950"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(116..>1641)
                     /locus_tag="VEA63_16095"
     CDS             complement(116..>1641)
                     /locus_tag="VEA63_16095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="excinuclease ABC subunit A"
                     /protein_id="HYD85590.1"
                     /translation="AHRFALQLGEAHGGNEAVREVVTGIEQRIRFLLETGLGYLTLDR
                     DYATLSGGEAQRVRLATQLGMGLMGVIYVLDEPSIGLHPHDNQKLLDTLVALRDRGNT
                     VLVVEHDEDTMRLADELIELGPEAGIEGGHVLFQGKPADCMKLSAKESRTGPYLARKL
                     GVLKDAVTKEPDGAWLTVREARANNLRGVDARFPVGLLTCVTGVSGSGKSTLVNDVLA
                     AAAARKLNGAKTLPGKHRHIENLDFFEKLVQVDQEPIGRSPRSNPATYIDLMGLLRDL
                     FAQVPLAKVRGYKASRFSFNVRGGRCERCQGDGVIKLDMQFMADAYAPCPSCGGRRFN
                     RETLEILFHGKSIADVLDMTVREAIGLFRNIPRVMDKLATLDAVGLGYLTLGQSATTL
                     SGGEAQRIKLSLELSKRQQGQTLYILDEPTTGLHWVDIQKLMDLLFKLRDAGNTVIVI
                     EHNLDVINLADWIIDLGPGGGRDGGEIVFAGTRKEIEASERSLTGEALRRWRAASRK"
BASE COUNT          303 a          600 c          426 g          312 t
ORIGIN      
        1 tatcacgtaa actgggcttc gccgctcgca tctcggatcg ccgcggagcg attgccatcg
       61 cgcgaaaaac aaatcctcgc ctccaggcgc ggctcggtcc tttcagctaa accttctatt
      121 tccggctcgc agcacgccag cgtctgagcg cctcgcccgt cagcgatcgc tcgctggctt
      181 cgatttcttt ccgcgttccc gcgaagacga tttcgccgcc gtcgcgtccg ccgcccggac
      241 cgagatcgat gatccagtcc gcgagattga tcacgtcgag attgtgctcg atcacgatca
      301 cggtgttgcc cgcatcgcgc agtttgaaga gcaggtccat caacttctga atatcgaccc
      361 agtgcagccc cgtcgtcggc tcgtcgagaa tgtagagcgt ctgcccttgc tggcgcttgc
      421 tcaactccag cgacagcttg atccgctggg cctcaccacc cgacaacgtc gtcgcggact
      481 gcccgagcgt cagataaccg agaccgaccg cgtcgagcgt cgcgagcttg tccattacgc
      541 gcgggatatt tcgaaacaac ccaatcgctt cgcgcaccgt catgtcgagc acgtcggcga
      601 tcgacttgcc gtgaaacaaa atttccagcg tctcgcgatt gaaccgccgc ccgccgcagc
      661 tcggacacgg cgcatacgcg tcggccatga actgcatgtc gagtttgatc acgccgtcgc
      721 cctggcagcg ctcgcagcgc ccgccgcgca cgttgaacga aaaacggctc gccttgtagc
      781 cgcgcacttt cgccagcggc acctgcgcaa acagatcgcg caacagcccc atcaaatcga
      841 tatacgtcgc cgggttcgaa cgcggactcc gtccgatcgg ctcctgatcg acttgcacga
      901 gcttctcgaa gaaatccaga ttctcgatgt gccggtgttt gcccggcagc gttttcgcgc
      961 cgttcaactt ccgcgccgcc gccgccgcaa gcacgtcgtt caccagcgtg cttttccccg
     1021 agccactcac gcccgtcacg cacgtcagca agcccacggg aaaccgcgca tcgacaccgc
     1081 gcagattatt cgcacgcgct tcacgcacgg tcagccacgc gccgtccggc tccttcgtga
     1141 ccgcgtcctt caacacgccg agcttccgcg cgagataagg ccccgtgcgc gattctttcg
     1201 ccgacagctt catgcagtcc gccggtttcc cctgaaacaa aacgtgaccg ccctcgatcc
     1261 ccgcttcggg cccgagttcg atcaactcgt ccgcgagccg catcgtgtcc tcgtcgtgtt
     1321 cgacgaccag caccgtgttt ccgcgatcgc gcaaagccac caacgtatcg agcagcttct
     1381 gattgtcgtg cggatgcagt ccaatgctcg gctcgtccaa cacgtaaatc acgcccatca
     1441 accccatgcc aagttgcgtc gcgagccgca cgcgctgcgc ctcgccgcca gacaatgtcg
     1501 cgtaatcgcg atcgagcgtg agatacccga gccccgtctc taacagaaac cgaatccgct
     1561 gctcgatccc ggtcacgact tcgcgcaccg cctcgtttcc gccatgcgcc tcgcccaact
     1621 gcagcgcgaa ccgatgcgcc t
//
LOCUS       DASPLT010001579         2992 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_19443, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001579 DASPLT010000000
VERSION     DASPLT010001579.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 2992)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2992)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2992
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_19443"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..883)
                     /locus_tag="VEA63_16100"
     CDS             complement(<1..883)
                     /locus_tag="VEA63_16100"
                     /inference="COORDINATES: protein motif:HMM:NF013104.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD85591.1"
                     /translation="MKLRSQHLSGALKMAGIFVSLAVVSALAQEAAAEAPKYADLAAF
                     AESPGYTIFTANNIWMMVCAALVFFMNLGFGCVESGLTQSKNTANIMFKNTIVPCIGI
                     VTYALVGFNLMYPGGDGGGWFGFAGVGLGFSDDVAGLTDVYNAGYTYWTDFLFQAMFA
                     ATAATIVSGAVAERIKLSSFMVFTVLLVTISYPITGMWKWGNGWLNTMETPFYDFAGS
                     TIVHSVGGWGALAGIMILGPRLGKFRDGKVMPILGHSIPLATIGVFILWLGWFGFNGG
                     SVLSANPGAVSLVLVTTC"
     gene            complement(1238..>2992)
                     /gene="aspS"
                     /locus_tag="VEA63_16105"
     CDS             complement(1238..>2992)
                     /gene="aspS"
                     /locus_tag="VEA63_16105"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007362736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HYD85592.1"
                     /translation="LADLDSTVSLLGWVDTIRDQGGIIFVDLRDRKGITQIKLEAHDN
                     SQLAEQLKHLKAESVIGITGKVTRRPEGTENKSLPTGEIEVTATSLEIHNISDTPPFP
                     LDDVGGDKVNEDLRLTYRYLDLRRPKMRKNLQVRHKAAKSIRDYFDSQEFIEVETPAL
                     FKSTPEGAREYLVPSRIHAGQFYALSQSPQQFKQILMVAGVEKYFQIARCFRDEDLRA
                     DRQMEFTQVDVEASFVTREDIYTLFEGMLKKLWKDVLNEDIATPFQRMPFHEAMNRFG
                     VDKPDLRFGLELVDFSETFKASAFKVFQSTVAAGGVIKAINAKGLADLTQGELKNLED
                     IAKSLGAKGLAFIKVEGGEWKSPIVKFFSDAEKAALTEKLGIAEGDMVFFAAAPWEKA
                     CAILGRLRLEAAQLLVKRGKMTIRHDDWKFLWVIDFPLMSHDEAENRYVATHHPFTAP
                     VPEDTQYLDTDPKKVRGQHYDVVLNGMELGGGSIRIHQPALQKKVFEDVLKIPADVVE
                     SRFGYMLKAFTYGAPPHGGIAFGLDRMCALLCGTTSIRDVIAFPKTQKGQDLMAQSPT
                     PVTPRQLKELHIQTVMPE"
BASE COUNT          637 a          961 c          808 g          586 t
ORIGIN      
        1 gacaggttgt caccagcacc agcgaaaccg caccgggatt tgcggagagc acggaaccgc
       61 cgttaaagcc gaaccagccg agccagagga tgaacacacc gatcgtcgcg agcgggattg
      121 aatgaccgag aatcggcatg acctttccat cgcggaattt tccgagacgc ggtccgagaa
      181 tcatgatgcc ggcgagcgca ccccagccgc cgactgagtg cacgatcgtc gaaccggcaa
      241 aatcgtagaa cggcgtttcc atcgtgttga gccagccgtt gccccatttc cacatgccgg
      301 tgatcggata cgaaatcgtc accagcagca cggtgaacac catgaatgaa ctcagcttga
      361 tgcgctcggc gaccgcgccc gaaacgatcg tcgccgccgt cgcggcaaac atcgcttgga
      421 agagaaaatc ggtccagtaa gtataaccgg cgttatacac gtccgtcagc cccgcgacgt
      481 catcgctaaa gcccaggcca actccggcga agccaaacca gccgccgcca tcgccgccgg
      541 gatacatcaa gttgaagccg acgagcgcat acgtgacgat accgatgcac ggcacgatcg
      601 tgtttttgaa catgatgttc gccgtgttct tcgactgggt caggccggat tccacgcagc
      661 cgaacccaag gttcatgaaa aacacgagcg cggcacagac catcatccaa atattattcg
      721 ccgtgaaaat cgtgtagccc ggagattctg cgaaggcggc gagatccgcg tatttgggtg
      781 cttctgcggc tgcctcctgc gctaaggcgc tgacgacggc gagcgagacg aagatgcctg
      841 ccatcttgag ggcgccggat agatgctggg accgtagctt catgataggt aagaacgaat
      901 ggtggttgaa aaaaggcgac gacctacccg atcgcgccgc acccaccaaa agcacggggt
      961 atgccaatgc acacgtccgt tccgcgcgcg ccgctgcaca gacattataa acactgtcta
     1021 tgcccgcttg catcgcgaat ggccgatcgc tctcgatctt cccgagcaac gttgcccgag
     1081 atcaggccta ccagattttc acgacggcgc agaatacgtc atgtatatac gacattgtat
     1141 ccgccacgaa agccgagcgc ttccactgcc aaaaacagag gcgggtccca agacccgccc
     1201 tgctctcctg caccaggtcc aaacgcactc gcgcgctcta ctccggcatc accgtttgaa
     1261 tatgcagctc tttaagctgc cgcggcgtca ccggcgtcgg gctctgcgcc atcagatcct
     1321 ggcccttctg cgttttcgga aacgcgatca cgtcgcgaat gctcgtggtg ccgcagagca
     1381 gcgcacacat gcgatcgagc ccaaacgcga tgccgccatg cggaggcgcg ccatacgtga
     1441 acgctttcag catataaccg aaacggctct ccacgacatc cgccggaatc ttcagcacgt
     1501 cctcgaacac tttcttctgc agcgcaggct ggtgaatgcg gatcgatccg ccgcccagct
     1561 ccatgccgtt caacaccaca tcgtagtgct gtccgcgcac cttcttcggg tccgtatcca
     1621 aatattgggt atcctcaggc accggcgccg tgaacggatg atgcgtcgcc acgtagcggt
     1681 tctccgcctc gtcgtggctc atcaacggaa aatcaatcac ccacaggaat ttccaatcgt
     1741 cgtgacggat cgtcatcttg ccgcgcttca ccagcaactg cgccgcctcg agccgcaacc
     1801 gtccgagaat cgcgcacgcc ttctcccacg gcgcagccgc gaagaacacc atgtccccct
     1861 cggcgatacc aagcttctcc gtcagcgcgg ccttttccgc gtcggaaaag aatttcacga
     1921 tcggcgattt ccattcgccg ccttcgacct tgatgaacgc gaggcctttc gcgccgagcg
     1981 atttcgcgat gtcctcgagg ttcttcaact cgccttgtgt gaggtccgcg agccccttcg
     2041 cgttgatcgc cttgatcacg ccgccggcag cgacggtcga ttgaaacacc ttgaacgccg
     2101 acgccttgaa tgtctcggaa aaatccacca gctcgagccc gaagcgcagg tccggcttgt
     2161 cgacgccgaa gcggttcatt gcttcgtgaa acggcatgcg ctggaacggc gtcgcgatgt
     2221 cttcgttcag cacgtccttc cacaatttct tcaacatccc ttcgaacagc gtgtagatgt
     2281 cctcgcgcgt cacgaacgac gcctcgacgt ccacctgcgt gaactccatc tgccggtccg
     2341 cgcgcagatc ctcgtcgcga aaacagcgcg cgatttggaa gtatttttcg acgcctgcca
     2401 ccatcaggat ctgcttgaat tgctgaggcg actgcgagag cgcataaaac tgccccgcgt
     2461 gaatgcgcga cggcaccaaa tactcgcgcg cgccttccgg cgtgctcttg aacagcgccg
     2521 gcgtctcgac ttcgatgaac tcctgcgaat cgaagtaatc gcgaatcgac ttcgccgcct
     2581 tgtgacgcac ttgaaggttc ttgcgcatct tcggccggcg cagatccaga taacgatacg
     2641 tgaggcgcag atcctcgttc accttgtcgc cgcccacgtc gtccagcgga aagggtggcg
     2701 tatccgaaat attgtgaatc tccagcgacg tcgcggtgac ttcgatttcg cccgtgggga
     2761 gcgatttgtt ttccgtgccc tcggggcggc gcgtgacctt gcccgtaatg ccgatcaccg
     2821 actccgcctt caaatgcttc agctgctcgg ccaactgtga attgtcatgc gcctcgagct
     2881 tgatctgcgt gatgcctttg cggtcgcgta gatccacgaa aatgatgccg ccttgatcgc
     2941 ggatcgtgtc cacccacccg agcagagaga cggtcgagtc gagatcggcg ag
//
LOCUS       DASPLT010001580         1288 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_58654, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001580 DASPLT010000000
VERSION     DASPLT010001580.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1288)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1288)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1288
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_58654"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1111
                     /locus_tag="VEA63_16110"
     CDS             <1..1111
                     /locus_tag="VEA63_16110"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012372894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase activity,
                     zinc-dependent [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase"
                     /protein_id="HYD85593.1"
                     /translation="DDPAILNPGDAIVKITSTAICGSDLHLYNGYMPTLEKGDILGHE
                     FMGEVVEVGANVRRLRVGDRVVVPFTISCGGCSHCARQQWSLCDNTNPNAWMAEKLMG
                     FAPAGLYGYSHMTGGYAGGQAQYARVPYADHGPIVIESGLADEKVLFLSDIFPTGYMA
                     AENCDIKPGQTVVAVWGCGPVGQFAIRSAFLLGAAKVYAIDSVPERLAMAEAAGAITI
                     EDGDDVLETLRELTGGRGPDAAIDAVGMEAHGSAYDHVKQTLKLETDRPYALRQSMRA
                     VRKGGVLSIPGVYGGLLDKVPFGAVFNKGVTLKMGQTHVQRYMKHLLSLVEEGKIDPS
                     FVVTHRVPLTRAPEMYETFVEKRDGCIKVVLDPAA"
BASE COUNT          250 a          321 c          469 g          248 t
ORIGIN      
        1 cgacgatccg gcgattttga atccgggcga cgcgatcgtg aaaatcacat cgacggcgat
       61 ctgcggatcg gacctgcatt tgtataatgg atacatgccg acgttggaga aaggcgacat
      121 cctcgggcac gaattcatgg gcgaagtcgt cgaagtcggc gcgaacgtgc gccggctgcg
      181 cgtgggcgat cgcgtggtcg tgccgttcac gatctcgtgc ggtgggtgct cgcattgcgc
      241 gcggcagcag tggtcgttgt gcgacaatac aaatccgaac gcgtggatgg cggagaagct
      301 aatgggtttc gcgccggcgg gactctatgg ctattcgcac atgacgggtg gttacgcggg
      361 cgggcaggcg caatacgcgc gcgtgcctta cgcggatcac ggaccgatcg tcatcgagag
      421 cgggctcgcg gatgagaagg tgttgttcct ctcggatatt tttccgacgg gctacatggc
      481 ggcggagaat tgcgacatca aaccgggtca aaccgtcgtg gccgtgtggg gctgcgggcc
      541 ggtgggtcag tttgcaattc gcagcgcgtt tctgctcggc gcggcgaagg tgtatgcgat
      601 cgacagcgtg ccggaacggc tggcaatggc ggaggccgca ggcgcgatca cgatcgagga
      661 tggtgacgat gtgttggaga cgttgcgcga actcacgggc gggcgcggtc cggacgcggc
      721 gatcgatgcg gtcggaatgg aggcgcatgg ctcggcctac gatcacgtga aacagacgct
      781 gaaactcgag accgaccggc cgtatgcgtt acgtcagtcg atgcgcgcgg tgcgcaaagg
      841 aggcgtgctg tcgatccccg gcgtctacgg cgggttgttg gacaaggtgc cgttcggcgc
      901 ggtgttcaac aagggcgtga cgttgaagat gggacagacg catgtgcagc gctacatgaa
      961 acatctgctt tcgctggtgg aggaggggaa gatcgatccg tcgttcgtgg tgacgcatcg
     1021 cgtgccgctg acccgtgcgc cggagatgta tgagacgttt gtcgagaaac gcgacggttg
     1081 catcaaggtc gtgttggatc cggcggcgtg agcgtgggga agcaccgcca tccaagctcc
     1141 aaccttcaag ctccagggaa acattgagga gaagcgaagg cgcggttcct tgggggcggg
     1201 agctttagga atgggcgcaa aaaaaggcgg gctgaagccc gccctacttt tgtgcgtatt
     1261 agaattttga ctacggcgag caagtcgt
//
LOCUS       DASPLT010001581         1988 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_142790, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001581 DASPLT010000000
VERSION     DASPLT010001581.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1988)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1988)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1988
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_142790"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(196..873)
                     /locus_tag="VEA63_16115"
     CDS             complement(196..873)
                     /locus_tag="VEA63_16115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009513202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF502 domain-containing protein"
                     /protein_id="HYD85594.1"
                     /translation="MSTDSLRPPASRLITFRNAFISGALLLAPLIVTVWAFRTIIDLV
                     GGTFRPIYRDYLPESLQRLPFFWDLLATLAVILFVTALGYLSQYVFGKFFFSLAERTI
                     QRIPGVGGLYSSVKQVVSTFGAQKRNLFSKVVLVEFPRQGLWSLGFLTNKFQGEPHQR
                     AGVEMWTVFIPTTPNPTSGFLVMVPVDGIRELDMSVGDGMKMIISGGAVIPPWPTVNA
                     SDVPSPP"
     gene            1064..>1988
                     /locus_tag="VEA63_16120"
     CDS             1064..>1988
                     /locus_tag="VEA63_16120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citramalate synthase"
                     /protein_id="HYD85595.1"
                     /translation="MSNAVKIYDTTLRDGTQGEGISFSVTDKLLIAQKLDQFGVDYIE
                     GGFPGSNPRDISFFAEAKKLKLTHAKLAAFGSTRRAGVKPEEDAQLRTLLESEMPVLT
                     IVGKTWLMHVHEILRTTPEENLAMIEDSVRYLVSQGREVIYDAEHFFDGYLDNPEYAL
                     RTLDAAARGGASNLTLCETNGGKLVPEVSRIVAAAVERFGGDKVGVHCHNDSGLGVAV
                     TLAGVDAGARLVQGTINGYGERVGNANLVTVLPNLILKMGKTAHCAPKLAEIRDLSLF
                     FDELANLRPDPKAPFIGHSAFAHKGGLHANAA"
BASE COUNT          417 a          513 c          733 g          325 t
ORIGIN      
        1 ggagaacgag gaggctgccg tgctcggggg agaaaacggt gagcgattga aatgcgtcgg
       61 tcggcgggcg tttcaagagc acgaaggcgt cggtgtgcag cggaagcgcg ggcatcgtgg
      121 gcggcggggc ggcgcgagac gcgggtgggc ggaaggagct gacgagcagg agaaagagaa
      181 cgagaaagag aacgatcagg gcggcgaagg gacatcgctc gcgttgacgg tcggccaggg
      241 cggaatcacg gcgccgccgg agatgatcat cttcatgccg tcgccgacgg acatatccag
      301 ttcgcgaata ccgtcgacgg gcaccatcac gagaaagccg ctcgtggggt tgggcgtggt
      361 ggggatgaag acggtccaca tctcgacgcc agcgcgctgg tgcggttcac cttgaaactt
      421 gttcgtgagg aaaccgagcg accagaggcc ttggcgggga aactcgacga gcacaacctt
      481 gctgaacagg ttgcgctttt gcgcgccgaa ggtggaaacg acctgtttta cggagctata
      541 gagaccgccg acgccgggga tgcgctggat cgtgcgttcg gcgaggctga aaaagaattt
      601 accgaagacg tattgggaaa ggtagccgag ggccgtgacg aaaaggatca cggcgagcgt
      661 cgcgagcagg tcccagaaga acgggaggcg ctgcaatgac tcgggcagat agtctcgata
      721 aatgggacgg aacgtgccgc cgacgaggtc gatgatggtg cgaaacgccc agacggtgac
      781 gatcaggggc gcgagcagga gcgcgccgct gataaaggcg ttgcggaacg tgatgagacg
      841 cgacgcgggc ggtcggagag aatcggtgga cattcccggc cacgctagcg gcggagagtc
      901 gagttacgca cggaaaaacg ggatgcggct gccgccagtg aatgacggaa gcgcgggcac
      961 acaggcgttc gcggccaacg tattcgctgc cgcatgacgc tgcgatgcgg ccgaaaagct
     1021 cccttgccag cggagcggcg gggctcaaat ctcgtttttc cccatgagca acgccgtcaa
     1081 gatctacgac accacgctgc gcgatggcac gcagggtgag ggcatcagct tttccgtaac
     1141 cgacaagttg ttgattgcgc aaaagctgga tcagttcggc gtcgactaca tcgagggcgg
     1201 gtttccgggc tccaatccgc gggacatctc gtttttcgcg gaggcgaaaa agttgaagct
     1261 gacgcacgcg aagttggcgg cgttcggctc gacgcgtcgc gccggcgtga agcccgaaga
     1321 ggacgcgcaa ttgcggacgt tgctggagag cgagatgccg gtgctgacga tcgtcggcaa
     1381 aacctggttg atgcatgtgc acgagattct gcgcacgacg ccggaggaga atctcgcgat
     1441 gattgaggac agcgtgcgct acctggtgtc gcagggccgc gaagtcattt acgatgcgga
     1501 gcactttttc gacggctacc tcgacaatcc tgagtatgcg ttgcgcacgc tcgacgcggc
     1561 ggcgcgcggc ggggcgagca atctgacgtt gtgcgagacg aatggcggca agctcgtgcc
     1621 ggaggtttcg cggatcgtcg ctgcggcggt ggagcgtttc ggcggggaca aagtcggcgt
     1681 gcattgccac aacgacagcg ggctgggcgt ggcggtgacg ctcgcgggtg tcgacgcggg
     1741 ggcgcggctg gtgcagggca cgatcaacgg ctacggcgaa cgcgtgggta atgctaatct
     1801 ggtgacggtg ttgcccaact tgatcttgaa aatgggcaag acggcgcatt gcgcgccgaa
     1861 gctcgcggag attcgcgatc tgtcgctgtt cttcgacgaa ctggcgaacc tgcggcctga
     1921 tccgaaagcg ccgtttatcg gacattcggc gttcgcgcat aaaggcggac tgcatgcgaa
     1981 cgcggcgc
//
LOCUS       DASPLT010001582         1088 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_120806, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001582 DASPLT010000000
VERSION     DASPLT010001582.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1088)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1088)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1088
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_120806"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1077)
                     /locus_tag="VEA63_16125"
     CDS             complement(<1..1077)
                     /locus_tag="VEA63_16125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HYD85596.1"
                     /translation="MYEGQFVFSQLMDFLPRREFNECVRRYGGNRRPRGFSCRDQFLC
                     LAFAQLTFRESLRDIETCLRALQPKLYHAGFRGRVSRSTLADASRAHDWRIFADFAQV
                     LIRRARKLYSDEALCVELAETVYALDSTTIDLCLSLFPWAPFRRRTGAVKLHTLVDLR
                     GNIPCFIHVSHGKTHDVTALDELPIEPGAIYVMDRGYVDFRRLKRFTGAGAFFVTRSK
                     NNLDFTCRARRQIDKTTGLRADQTIVLAGPKSPRLYGEPLRRVRFFDAASEQRFTFLT
                     NNFAFPALTIAQLYKSRWQVELFFKWIKQHLRIKAFYGNSVNAVKTQIWIAISVYVLV
                     AIVKKEPRLDRSLHEILQILSLTLF"
BASE COUNT          242 a          322 c          323 g          201 t
ORIGIN      
        1 gaaaagggtg aggctgagga tttgcagaat ttcgtgcagg ctgcggtcca gcctcggttc
       61 ctttttcacg atcgccacga gcacgtacac gctgatggcg atccagattt gggtcttcac
      121 ggcgtttacg ctgttgccat agaaagcttt gatccgcaga tgttgcttga tccacttgaa
      181 gaacaactcg acctgccatc gactcttgta aagctgcgcg atcgtcagcg cgggaaacgc
      241 gaagttgttc gtcaggaagg tgaatcgctg ctcgctcgca gcatcgaaga atctcacgcg
      301 gcggagtggc tcgccataga gccgcggcga tttgggaccc gcgagcacga tggtttgatc
      361 ggcgcgcagt cccgttgtct tgtcgatctg gcgtcgagcg cgacaggtga agtccaggtt
      421 gttcttgctc cgcgtcacga agaatgcgcc cgcgccggtg aagcgcttga gacgccgaaa
      481 gtcgacgtag ccacggtcca tgacgtagat cgctccgggc tcgatcggca actcgtcgag
      541 cgcggtcaca tcgtgcgttt tcccgtgcga aacgtggatg aaacagggaa tgttgccgcg
      601 caggtcgacc agcgtgtgca gcttcaccgc gcctgtgcgg cgccgaaacg gcgcccaagg
      661 aaacaaactc aagcagaggt cgatggtggt cgaatcgagc gcgtaaaccg tttccgcaag
      721 ctcgacgcag agcgcttcgt ccgagtacaa cttccgcgca cgacgaatca gcacctgcgc
      781 gaaatcggca aagatccgcc aatcgtgcgc gcgactggcg tcggccaagg tgctccgcga
      841 aacgcgtccg cgaaagccgg cgtgatacaa cttgggctgg agcgctcgca gacaggtttc
      901 gatgtcgcgc aaactctcgc gaaacgtcag ctgcgcaaat gccagacaca aaaactgatc
      961 gcgacacgag aaaccacgcg gccgtcgatt gccgccatac ctgcgaacgc actcgttgaa
     1021 ctcgcgccgc ggcaggaaat ccatcaactg cgaaaacacg aactgacctt cgtacacaag
     1081 acctcccc
//
LOCUS       DASPLT010001583         1178 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_142805, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001583 DASPLT010000000
VERSION     DASPLT010001583.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1178)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1178)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1178
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_142805"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..843
                     /locus_tag="VEA63_16130"
     CDS             <1..843
                     /locus_tag="VEA63_16130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HYD85597.1"
                     /translation="YTVGVLNNQPMGFYSPATLLQDGRRHRLKAHPVCIAHSNWECTV
                     EGEKTIRIGLRYVKGLRQTAVDAMLVQRRATPFGSMDDFLRRTDFTAVERRALASAGA
                     LNALTLHRRAALWQIEAAWSDEENLFKHFAETERTDSPLTPMTLSERLAADFQLLSLT
                     TGRHPMALLRDRLPGVHRAKDLKETKTGERVTIAGSVICRQRPGTAKGFVFVSLEDET
                     GIANAVVVPALFERHRLLITQESALRITGVVQNVSNVIHIKAQHIEALREEMLPAQAS
                     HDFH"
     gene            871..1128
                     /locus_tag="VEA63_16135"
     CDS             871..1128
                     /locus_tag="VEA63_16135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD85598.1"
                     /translation="MSVRCMRHRDLIPYKDRSGDSGVRAYTIETDAIIVEFKSGGGYR
                     YDSTAPGRRHVAAMKKLAQSGDGLATYINQHVRDDYAEKLW"
BASE COUNT          245 a          414 c          306 g          213 t
ORIGIN      
        1 tacaccgtcg gcgtgctgaa caaccagccg atgggcttct actcgcccgc cacgctcctg
       61 caggacggcc gccggcacag attgaaagcc catcctgtct gcatcgcgca ctcgaactgg
      121 gagtgcactg tcgaggggga aaaaaccatc cgcatcggtc tgcgctatgt gaaaggtctg
      181 cgccaaaccg ccgtcgacgc gatgctcgtt cagcgtcgcg caacgccgtt cggctcgatg
      241 gatgattttc tccgccgcac cgattttacc gccgtcgaac gacgcgcgct tgcgtccgcc
      301 ggcgcactga acgcgctcac ccttcaccgt cgcgccgcct tgtggcagat cgaagccgca
      361 tggtccgacg aagaaaatct cttcaagcac tttgccgaaa ccgagcgcac cgattcgccg
      421 ctcactccca tgacactctc cgaacgcttg gcggcggatt ttcaacttct gtccctcacc
      481 acgggacgtc accccatggc acttctccgc gatcgcttgc ccggcgtgca ccgcgccaag
      541 gatctcaagg agacgaaaac cggcgaacgc gtcactatcg ccgggtccgt catctgccgt
      601 cagcgtcccg gcaccgccaa aggcttcgtc ttcgtcagcc tcgaggacga aaccggtatc
      661 gccaacgccg tggtcgttcc cgcgctgttc gagcgccatc gcctgctgat cacacaggaa
      721 tccgcactgc gcatcaccgg cgtggtgcaa aacgtctcga acgtcattca catcaaagcc
      781 cagcatatcg aagcgctgcg ggaggaaatg ctgcccgcgc aagcctcgca cgatttccac
      841 tgagccgaat cgcactccaa catcagacgc atgtccgttc gctgcatgcg ccatcgggat
      901 ttgatacctt acaaagaccg cagcggcgac tccggcgtgc gcgcctatac gatcgaaacg
      961 gatgccatca tcgtggaatt caaaagcggc ggcggctacc gctacgactc caccgctccc
     1021 ggtcgacgcc acgtcgctgc gatgaaaaaa ctcgcccaat caggtgacgg tctcgcgacc
     1081 tacatcaacc aacacgtgcg agacgactac gcggaaaagc tctggtgaac ttggcccggc
     1141 ctattccacg taatcgatcc cgcttatcac cacctgct
//
LOCUS       DASPLT010001584         1994 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_265389, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001584 DASPLT010000000
VERSION     DASPLT010001584.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1994)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1994)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1994
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_265389"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1304)
                     /locus_tag="VEA63_16140"
     CDS             complement(<1..1304)
                     /locus_tag="VEA63_16140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HYD85599.1"
                     /translation="MMIRSYLVLAFALISVRLHAAPLSFGSATSLDDTWAFSLHDDPT
                     AKDPAFDDRAWERVTVPHDWSVKAPLSPELASCTGYLPGGVGWYRRKIFIPAKGGEKV
                     FLYFEGVYNRSEVYINGHLLGKRPNGYVSFYYDATPRVKFDEENTIAVRVDHSRSADS
                     RWYTGSGIYRHVFMIRSGPVHLAPWGVFIRSRDADATSATLDVDAELQNESTEDVRVS
                     LEHKLLDAGGGVAATAREEVNLRANSTVTTTHALTVSEPKLWRLDAPHLYRLHTTLVH
                     ENGPTLDQTTVTTGIRTLTFDPNLGFALNGDWMKMKGVCLHHDAGVLGAAVPRAVWRQ
                     RLETLKALGCNAVRMSHNPQAPDVYELCDELGLLVLDEAFDEWEFPKRKWLEGWNVGT
                     PGLEGSADFFEEWSARDLADMIRRNRNHPSIFAWSIGNEVDYP"
     gene            complement(1412..>1994)
                     /locus_tag="VEA63_16145"
     CDS             complement(1412..>1994)
                     /locus_tag="VEA63_16145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD85600.1"
                     /translation="LHPETTPLRKAIDLCWEKQDLRASLSPGGQALGWMLASDGRTRW
                     HNGMTGGFHAALFVSRELRVATIFLCNRSTPVGTEVAENLVRRAAGQPERAVPNRDRA
                     TVALTTDQLDRCTGTFRISPQFALVVERRNQALFVTPTGQSTDRLYAAAPDTFFSRRV
                     AAELVFDLPTDGGGATALTLKQSGRQARAVREP"
BASE COUNT          384 a          618 c          631 g          361 t
ORIGIN      
        1 ggatagtcga cttcgttgcc gatgctccag gcgaagatcg acggatggtt tcggttgcgg
       61 cggatcatgt ctgcgagatc gcgcgcgctc cactcctcga agaagtcagc ggagccctcc
      121 aagccaggcg tgccgacgtt ccatccttcg agccatttcc gtttcggaaa ctcccattcg
      181 tcgaacgctt cgtccaacac cagcagtcca agttcgtcgc agagttcgta gacgtcggga
      241 gcctgcggat tgtggctcat gcgaaccgca ttgcacccga gtgctttcaa cgtttccaaa
      301 cgctgtcgcc aaaccgcccg cggcacggca gcgcccagca cgcccgcgtc gtgatgcaga
      361 cacacgcctt tcatcttcat ccagtcgcca ttcagcgcga agccgaggtt gggatcgaag
      421 gtcagcgtgc gaatcccggt cgtgaccgtg gtttgatcga gcgtcgggcc gttttcgtgc
      481 acgagcgtgg tgtgcaaccg atacaagtgc ggcgcgtcca atcgccagag cttcggctcg
      541 gacacggtca acgcgtgcgt cgtggtcacc gtggagttgg cgcgcaggtt aacttcctct
      601 cgcgcagtcg cggccacacc gccgccggca tccagcaatt tgtgctcgag gctcacgcgg
      661 acgtcttccg tcgattcgtt ttggagttcg gcatcgacgt cgagagtcgc tgacgtggcg
      721 tcggcgtcgc gcgagcgaat aaacacgccc cacggtgcga ggtgcaccgg cccggagcgg
      781 atcatgaaga cgtgccgata aatgccagac cccgtatacc accgcgaatc ggcggagcgg
      841 ctgtggtcga cgcgcacggc gatcgtgttc tcctcgtcga acttcacgcg cggcgtggca
      901 tcgtagtaaa acgacacgta gccgttcgga cgtttcccca acaggtgacc gtttatataa
      961 acttcgctgc ggttataaac accttcgaaa tagaggaaga ccttttcgcc gcccttcgcg
     1021 ggaatgaaaa ttttacggcg ataccagccc acgccgcctg gcagataacc cgtgcagctc
     1081 gccaactcgg ggctgagcgg agccttcaca ctccagtcat gcggcaccgt cacgcgctcc
     1141 cacgcgcggt cgtcaaacgc gggatctttc gccgtggggt cgtcatggag cgagaacgcc
     1201 caggtatcat cgagcgaagt ggccgagcca aaggacaacg gcgcggcgtg cagacgcact
     1261 gatatcagtg cgaacgcgag aacgaggtaa gaccgaatca tcatagggga ggggcggacg
     1321 cgcatcgtaa cacgaattcc gcctccgcaa taggtgcggc ttcaccgctg cgcgaaaatc
     1381 acccgatcgg gtgagcgtgg agttcgcacc gctatggctc gcggacggcg cgagcctgcc
     1441 ggccagattg cttcagcgtc aaagccgtcg caccgccgcc gtcggtagga agatcaaaaa
     1501 ccagttcggc ggcgacgcgt cgggagaaaa acgtgtcagg cgccgccgcg taaaggcgat
     1561 ccgtggattg gccggtgggg gtgacgaaca gcgcttgatt gcggcgctca accacgagcg
     1621 cgaactgcgg gctgatccga aacgtgcctg tgcaacgatc gagttgatcg gtcgtcagtg
     1681 cgaccgtcgc acgatcgcga ttcgggacgg cgcgttccgg ttggccggcg gcgcggcgga
     1741 ccaagttctc cgcgacttcc gtgccgacgg gcgtcgaacg attgcacagg aagatggtcg
     1801 ccacgcgcag ctcccgactt acgaagagcg cggcgtgaaa gccgcccgtc atgccgttgt
     1861 gccagcgcgt gcgaccgtcg ctggcgagca tccatcccag cgcttgtccg ccgggtgaga
     1921 ggctcgcgcg gagatcctgt ttttcccagc agagatcgat ggccttccgc agcggagtcg
     1981 tttccggatg aagc
//
LOCUS       DASPLT010001585         1079 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_19496, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001585 DASPLT010000000
VERSION     DASPLT010001585.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1079)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1079)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1079
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_19496"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..300)
                     /locus_tag="VEA63_16150"
     CDS             complement(<1..300)
                     /locus_tag="VEA63_16150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD85601.1"
                     /translation="MPTLATTVDGLKLPNPFIIGSGPPGTNLSVISRAFREGWGAVIA
                     KTVSLDASKVVNVSPRYAKLHASSGEVIGWENIELISDRPFHLWLDEFKKCKDLHP"
     gene            512..>1079
                     /locus_tag="VEA63_16155"
     CDS             512..>1079
                     /locus_tag="VEA63_16155"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HYD85602.1"
                     /translation="MPLDPQRTIAELKELRALTGDDNGAQRVAFTPMWLAARAWLRRK
                     LEALPVEVHTDAAGNLWATLRGESSHALLMGGHIDSVPNGGWLDGCLNTLAGLEVLRR
                     VNEEFGGKPPVTVRLVDWADEEGARFGKSLFGSSACAGSLNMEEARGLKDRAGVALPE
                     ALKAVGIEFERVKESGVELKDAAAYLELH"
BASE COUNT          197 a          258 c          423 g          201 t
ORIGIN      
        1 ggggtggagg tccttgcact ttttgaattc gtcgagccag aggtgaaaag ggcggtcgct
       61 gatcagctcg atgttttccc agccgatgac ttcgccggat gacgcgtgga gtttggcgta
      121 gcgcggggaa acgttgacga ctttcgatgc gtcgaggctg acggttttcg cgatgacggc
      181 gccccagcct tcacggaagg cgcggctgat cacgctgagg ttggtgccgg gagggccgga
      241 gccgatgatg aacggattgg gaagtttcag accgtcgacg gtggtggcga gcgtgggcat
      301 aagaagaagg gatgaatgaa ctggagcagg agacgtgcga gccgtgagag accgggccgc
      361 caagagcgtc tgcgcggtgc gggatgaact gccggattga aggtggcgtg gaggttgcta
      421 atttccaagc tgcaaacggg cgcgggtttt gcgttggctg gaaatgcagg cgctcgtttt
      481 gtcgagcgta agcctccctc ttatttcaac catgccactc gatccgcagc gcacgattgc
      541 cgaattaaag gaattgcgcg cgctgacggg tgacgacaac ggcgcgcagc gcgtggcgtt
      601 cacaccgatg tggctggcgg cgcgagcgtg gttgcggcga aagctcgaag cgctgccggt
      661 ggaagtgcac acggacgcgg cgggaaacct ctgggcgacc ttgcgcggcg agtcgtcgca
      721 cgcgctgctg atgggcgggc acatcgactc ggtgccgaat ggcgggtggc tcgacgggtg
      781 tctgaacacg ctcgcggggc tcgaagtgtt gcgtcgcgtg aacgaggagt ttggcggaaa
      841 accgccggtg acggtgcggc tcgtcgattg ggcggacgag gaaggcgcgc gtttcggcaa
      901 gagcttgttc ggttcgtcgg cgtgcgcggg atcgttgaac atggaagagg cgcgggggtt
      961 gaaggatcgc gcgggcgtgg cgttgccgga ggcgttgaag gcggtcggca tcgaattcga
     1021 acgcgttaaa gagagcggcg tggagttgaa ggacgcggcg gcgtatctcg agctgcaca
//
LOCUS       DASPLT010001586         1425 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_223673, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001586 DASPLT010000000
VERSION     DASPLT010001586.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1425)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1425)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1425
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_223673"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..249
                     /locus_tag="VEA63_16160"
     CDS             <1..249
                     /locus_tag="VEA63_16160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HYD85603.1"
                     /translation="GLCNCAERTALFTAAAAGEREIAAVVVYTPTPTPTSPCGACRQV
                     INEFGPHALVISVCDGPDVREATLDALLPDAFGPADLA"
     gene            complement(415..>1425)
                     /locus_tag="VEA63_16165"
     CDS             complement(415..>1425)
                     /locus_tag="VEA63_16165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007361646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HYD85604.1"
                     /translation="FSIAEYYRYERAQKGRERAFFQFNADLFGEPGPEAEIELIGLLI
                     QCLRAFGLSEEDFYVRLSDRNLWFFYLEALGLDEPRIRAVLTAVDKFEKIGDEAFKPY
                     GEQFGAIDSALKQKILAFLEIKTLTVLEQTLGDLGGEKIAGRLADWRKLLGALGAMGL
                     SKFVEVDLGVVRGLAYYTGFVFEAFDRKGELRAIAGGGRYNDLVKKLGGPDLPAVGFA
                     IGDVTLGLLLEARGLMPAMVQACDVYCVVGGEAERRAAFGDVFALRAAGFRTEYPLKE
                     VAFGKQFKLAADSGAKLALIYGADELAKGVVKIRNLTDRSEVEVPREQVLANVRDFFS
                     GS"
BASE COUNT          287 a          520 c          365 g          253 t
ORIGIN      
        1 ggcctctgca attgcgccga acgcaccgcg ctctttaccg ccgccgcggc cggtgaacgc
       61 gagattgccg ccgtcgttgt ctacacgccc actccgacgc cgacgtcgcc ttgcggcgcc
      121 tgtcgccaag tcatcaacga attcggtccg cacgcgctcg tgatcagtgt gtgtgatggt
      181 ccggacgtcc gcgaagccac cctcgacgcg ctgctgcccg acgccttcgg cccggccgat
      241 ctcgcctgac gccttcgcgt ttcaggtccg gaggcaggag cctgcttgca ggagatttca
      301 aggtgcggac aaccagcatc gccccgaagc aggctttcac aacggacatg acatcaggac
      361 cgcccggagg tcggctccta cctatccaac tcagaacagc acacgcgcca cgtttcagct
      421 tccgctgaaa aaatcgcgca cgttcgccaa cacctgctcg cgcggcactt cgacttcgga
      481 gcgatccgta agattgcgaa tcttcacgac gcccttcgcg agttcgtccg ccccgtaaat
      541 cagcgccagc ttcgcacccg aatccgccgc cagcttgaac tgctttccaa acgccacctc
      601 cttcaacgga tactccgtgc gaaagcccgc cgcgcgcagc gcgaaaacgt cgccgaaggc
      661 cgctcgacgt tccgcctcgc caccgacaac gcagtaaacg tcgcacgcct gcaccatcgc
      721 cggcatgagc ccgcgcgctt cgagcagcag tccgagtgtc acatcgccga tcgcgaaccc
      781 caccgccggc aaatccggtc cgccgagttt tttcaccaga tcgttgtagc gcccaccgcc
      841 cgcgatcgca cgcaactcgc ccttgcgatc gaacgcctcg aacacgaagc cggtgtaata
      901 ggccagcccg cgcacgacgc cgagatcgac ctccacgaat ttcgacaagc ccatcgcacc
      961 caatgcaccg agcagttttc gccagtcggc gagacgcccc gcgatttttt ccccgcccaa
     1021 atcgcccaga gtctgctcga gcaccgtgag cgttttgatc tcgaggaacg ccaggatttt
     1081 ttgcttcaac gccgagtcga tcgcgccgaa ctgttcgcca taaggcttga aggcctcgtc
     1141 gccgatcttc tcgaacttgt ccacggctgt gagcaccgcg cgaatccgcg gctcatccaa
     1201 acccaaggct tcgagataaa aaaaccacag gttgcggtcg ctcaaccgga cgtagaaatc
     1261 ttcctcgctc agcccgaacg cacgcaggca ttggatcaac aagccgatca gctcgatctc
     1321 cgcttccggc cccggctcgc cgaaaagatc cgcgttgaac tgaaaaaacg cccgctcgcg
     1381 ccctttttgc gcccgctcgt agcgataata ttctgcgatg ctgaa
//
LOCUS       DASPLT010001587         1139 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_243541, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001587 DASPLT010000000
VERSION     DASPLT010001587.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1139)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1139)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1139
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_243541"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            290..445
                     /locus_tag="VEA63_16170"
     CDS             290..445
                     /locus_tag="VEA63_16170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD85605.1"
                     /translation="MLSLMPLLAISGVIAYVFATLKMLTAAIEAPVGYENDEGFHYGI
                     DVGDAER"
     gene            538..690
                     /locus_tag="VEA63_16175"
     CDS             538..690
                     /locus_tag="VEA63_16175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD85606.1"
                     /translation="MLPILTLVLSGVVGYAFVALKMMRAVSEAPLGYEDEAGFHYGKP
                     TDSTQS"
     gene            871..>1139
                     /locus_tag="VEA63_16180"
     CDS             871..>1139
                     /locus_tag="VEA63_16180"
                     /inference="COORDINATES: protein motif:HMM:NF036808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD85607.1"
                     /translation="MPDVFSLKLKPNAKPRVLAGHPWVFANEVEALLPAENDGAVVEC
                     RDRAGRFLGTGIYNSKSQIVWRRLSRERVALDEAFVRGAIERAVAR"
BASE COUNT          226 a          273 c          402 g          238 t
ORIGIN      
        1 cggatttcat cgctgcgctg cgaacgggga aacggcccga tggaagcgta ttgagcccgg
       61 tgatgccggc ggcattgagc ggcttaaacg acgtcgagat gaaggcgatg tggatgtttt
      121 taaagacgct gccgccgctg ccgacgggaa cgcgttaatt tatttacctt ctggagtgtg
      181 tggcgcgacg ttgcggcgaa aagttccgcg gcggtgtaac aaacgcgtca agatattcca
      241 aatacgtgga gacgactcgc aatttgggaa aagtcattac ggttaagtca tgctttccct
      301 tatgcccctc ctcgcgattt ccggtgtgat cgcttacgtg tttgcaacct tgaagatgct
      361 gaccgcggcg atcgaagcgc cggtgggtta cgagaacgac gagggatttc actacgggat
      421 cgacgtcggc gacgcggaac gttgagcggc gtctgaggtg cgaagcggga gcgaaagaaa
      481 attcgggaaa ttcgcgggat ccactaaagt cacggatcgg tgggccgaaa aggtggtatg
      541 cttccgatcc ttaccctggt tctctccggt gtggttggtt acgcgtttgt cgcgttgaag
      601 atgatgcgag cggtgtcgga agcgccgctc ggctacgaag acgaggcggg ctttcattac
      661 ggcaagccga ccgactcgac gcagagctaa gctcgcgatc gttttaagcg cgatcaagtg
      721 cgagatgcga ggttgatcgg cgtttgcggg tgtcgcgagt gaagtaggcg cacggcaagc
      781 gcaggacgca gaagtggcca cagcaagtgg gcgcgaggac gcactccttt ttttcgtcgc
      841 ggccggcgac aatcgcgcaa gcgtgggcgg gtgcctgatg ttttttcgct gaagctgaaa
      901 cccaacgcga agccgcgtgt gctcgccgga cacccgtggg tgtttgcgaa tgaagtggag
      961 gcgttgctgc ccgcggagaa cgatggagcg gtcgtggaat gtcgcgaccg tgcgggccgg
     1021 tttctcggga cgggaattta caactcgaag tcgcagattg tgtggcggcg cttgagtcgg
     1081 gagcgcgtgg cgttggacga ggcgttcgtg cgtggagcga tcgaacgcgc ggtggcgcg
//
LOCUS       DASPLT010001588         1509 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_100146, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001588 DASPLT010000000
VERSION     DASPLT010001588.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1509)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1509)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1509
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_100146"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..252
                     /locus_tag="VEA63_16185"
     CDS             <1..252
                     /locus_tag="VEA63_16185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD85608.1"
                     /translation="VGRGPTPSAGALTSPRIETWGLVLQQLAVAPLLVGAWLMDERWL
                     SAGVWLLAAGVVLFSADMLGVLKHLWIAGAAAPKTEKKS"
     gene            249..560
                     /locus_tag="VEA63_16190"
     CDS             249..560
                     /locus_tag="VEA63_16190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HYD85609.1"
                     /translation="MSKILDADAVWEALRGIPDPEFGVNIVDLGLIYDVACNDGEVAV
                     TMTLTTPSCPSGGWIYEGAKEAIERLPGAKKVEVAMVFDPQWTPEMLSESAQRQLGRI
                     D"
     gene            complement(861..>1509)
                     /locus_tag="VEA63_16195"
     CDS             complement(861..>1509)
                     /locus_tag="VEA63_16195"
                     /inference="COORDINATES: protein motif:HMM:NF013226.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="protoheme IX farnesyltransferase"
                     /protein_id="HYD85610.1"
                     /translation="RARPLPQATLSPHEALAWSLAFSFGGVTLLAVGTNLLAAAFAAA
                     TIFLYGVVYTPLKRRTRWATEIGSLSGALPALLGNAAAGDLASRPGLALTAILLLWQM
                     PHFFAIGWRHRVDYRAAGFPLLPAIDATGARTAKWSLGYALALAVVSLAPCALGWFGI
                     CYAIATVIANVWFLRCAWQFLREPDRDRGARSLFFASLGYLPLVMGALLIEQFFK"
BASE COUNT          292 a          376 c          591 g          250 t
ORIGIN      
        1 gtgggacgcg gtccgacgcc gtcggcgggg gcgttgacga gcccgcggat cgaaacgtgg
       61 gggctggtgt tgcagcagct cgcggtcgca ccgctgttgg tgggcgcgtg gctgatggat
      121 gagcgctggt tgagcgcggg cgtgtggttg ctggcggcgg gcgtggtgtt gttcagcgcg
      181 gacatgctcg gcgtgttgaa acatctttgg atcgccggcg ccgcggcgcc gaaaacggag
      241 aaaaaatcat gagcaagatt ctggatgctg acgcggtgtg ggaggcgttg cgcgggattc
      301 ccgatccgga gttcggggtc aacatcgtgg acctcgggct gatctacgac gtggcgtgca
      361 acgacggcga agtcgcggtg acgatgacgt tgacgacgcc gagttgtccg tcgggcggtt
      421 ggatttacga aggcgcgaag gaagcgatcg agcgcctgcc cggtgcgaag aaggtggagg
      481 tcgcgatggt gttcgatccg cagtggacgc cggagatgtt gagcgaatcg gcgcagcggc
      541 agttgggtcg gatcgattga ggcgaggatt tttttcaacc acggatgaac acggatggac
      601 acggattcga tccgtggggg tttcgcgcgg cgtcagggaa tttgatttta accacgaatg
      661 gacacgaagg gacacgaatg aaaaaccgag ttggaaagcg gctctgatgg ggccaccgaa
      721 cggggagggg taaatgctgt ctgccggctc ctctgaattc gtgttcattc gtgtccattc
      781 gtgatttctc ttctgcctga gcgcgggtgg tcggttcact ccaaagtcgt gaaatccgat
      841 gacgacgttc gcccatggaa ctacttgaag aattgttcga tgaggagggc gcccatgacg
      901 agggggaggt agccgaggga ggcgaagaag aggcttcggg cgccgcgatc gcggtcgggt
      961 tctcttaaaa attgccaggc gcatcggagg aaccagacgt tcgcgatcac ggtggcgatg
     1021 gcgtagcaaa tgccgaacca gcccagtgcg cacggcgcga gcgagacgac ggcgagcgcg
     1081 agcgcatagc cgagcgacca tttggcggtg cgcgcgccgg tggcgtcgat cgcggggagc
     1141 aacgggaaac cggcggcgcg gtaatcaacg cgatggcgcc agccgatcgc gaagaaatgc
     1201 ggcatctgcc agagcagcaa gatcgcggtg agcgcgaggc cggggcgcga agcgagatcg
     1261 ccggcggcgg cgttgccgag cagcgcgggc agggcgccgg agagcgaacc gatttcggtg
     1321 gcccagcggg tgcggcgttt cagcggcgtg taaacaacgc cgtagaggaa aatggtggcg
     1381 gcggcgaagg cggcagcgag gaggttggtg ccgacggcga gcagcgtcac accgccgaac
     1441 gaaaacgcga ggctccacgc caacgcttca tggggcgaga gcgtcgcttg cgggagcggg
     1501 cgggcgcgg
//
LOCUS       DASPLT010001589         1316 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_265544, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001589 DASPLT010000000
VERSION     DASPLT010001589.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1316)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1316)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1316
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_265544"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(266..>1316)
                     /locus_tag="VEA63_16200"
     CDS             complement(266..>1316)
                     /locus_tag="VEA63_16200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD85611.1"
                     /translation="TREIIALIERVLKERPDHPGANHALIHLVEAGPEPARGLASAEQ
                     LKFYAPDAGHLVHMPSHIFMRTGLYHEAALANERAAKADRGYITECQIQGFYPGMYYP
                     HNEHFLWWAFTFEGRRAEAFAKAGEIVTLANSPMCGTPIAEKPRFTHLRLLTAVRFAD
                     WDSILAEAEPAKEEALDRVMWHWARATALAALGDAEKADHEAAAARALADGEGVKAMD
                     NAYLPAIKIAGIAAQLAEGRAALARGEAESALTALRAAVALEDAMPYMEPAFWFYPTR
                     HTLGAALLSLDRADEAATVFRDDLRYWPDNGWALNGLALALEKQGDKEAAAKTRRRFE
                     KAWQHADVQPTLAMY"
BASE COUNT          234 a          475 c          367 g          240 t
ORIGIN      
        1 cgatagtagc ggcgcaacgc ttcgcgcccg ttcgcatgtc gcagcaaatc gaacgccgca
       61 aagtagggca gctcgaagcc gtcctgatag atcagggtcg tctcgatgcc gccgtcggtc
      121 agaaaaagct cgtccgcaag ctgcggaagc gcatggcgat atttcgcttt cataaatggt
      181 cttggttgtg cgtttcaaag tcaccagtgg cccaccactc ctcgttcaag aggagcgccg
      241 cgggcacgct gccgtgtcca aagtttcaat acatcgcgag cgtcggctgg acgtcggcat
      301 gctgccaggc cttctcgaaa cgccggcgcg ttttcgctgc cgcttccttg tcgccctgct
      361 tttccagcgc gagcgccagt ccgttcaacg cccagccatt gtccggccag taacgcaggt
      421 cgtcgcgaaa caccgtcgcc gcctcgtccg cccgatcgag cgacagcaac gccgcgccga
      481 gcgtgtgccg cgtcggataa aaccaaaacg ccggctccat gtaaggcatc gcatcttcga
      541 gcgcgaccgc agcgcgcaac gccgttaacg cgctctccgc ttcgccgcgc gccagcgccg
      601 cacggccttc cgccagctgc gcggcgatcc ccgcgatctt gatcgcagga agatatgcgt
      661 tgtccatcgc cttcacccct tcgccgtccg ccagcgcacg cgccgccgcc gcttcgtgat
      721 ccgctttctc cgcatcgccg agcgctgcca gagccgtcgc acgcgcccag tgccacatca
      781 cgcgatcgag cgcctcctcc ttcgccggct ccgcctccgc gagaatcgag tcccaatcgg
      841 caaaacgcac cgccgtgagc aggcgcaggt gcgtgaaacg cggcttctcc gcgatcggcg
      901 tgccgcacat cggcgagttc gcgagcgtca cgatttcgcc cgccttcgcg aaggcttccg
      961 cgcgacgccc ttcaaacgta aacgcccacc agaggaaatg ttcgttatgc ggataataca
     1021 tcccgggata aaagccctgg atctggcact cggtgatata accgcgatcc gccttcgccg
     1081 cccgctcgtt cgccagcgcg gcttcgtgat acagccctgt ccgcatgaaa atatgcgacg
     1141 gcatgtgcac gagatgcccc gcatccggcg cgtagaactt caactgctcc gcactcgcga
     1201 gcccccgcgc cggctccggc cccgcttcga ccagatggat caatgcgtga ttcgcaccgg
     1261 gatgatcggg cctttctttc agcacgcgct caatcagcgc gatgatctct cgcgtc
//
LOCUS       DASPLT010001590         1136 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_183804, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001590 DASPLT010000000
VERSION     DASPLT010001590.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1136)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1136)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1136
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_183804"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(53..>1136)
                     /gene="creD"
                     /locus_tag="VEA63_16205"
     CDS             complement(53..>1136)
                     /gene="creD"
                     /locus_tag="VEA63_16205"
                     /inference="COORDINATES: protein motif:HMM:NF008712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cell envelope integrity protein CreD"
                     /protein_id="HYD85612.1"
                     /translation="TAYFLPEELTIAGEVTPEVRRRGIYETVVYSSALKLGGEVRPDF
                     VAAGIEADRIDWEKARVFLGVSDLRGIRSVGALNAGGEAFAFESAETAAGAPYPLVAK
                     VTNVRPGAKLTFSVALSVQGTGRLDLAPIGKATTAKLESAWADPSFVGASLPLTRHVS
                     AGGFAAEWQSSHLSRGFPQSWTNRLVDDGEAISRIAASAFGVNFLQPVDGYCLVERAQ
                     KYGLLFFVLVFAVFFLFETTAALRIHPLQYAIVGVALSLFFLGFLALSEFLRTELAYG
                     IAAAVCTAMVALYAHTFLRTGRRTLVIGAGLGATYGYLFFVLKSQDYALLAGTAALFG
                     ALALLMFFTRRINWYAVDAERAATSA"
BASE COUNT          243 a          434 c          298 g          161 t
ORIGIN      
        1 ccagggttcg ttattgtaag gcgctccgga gaggtttcac cgggcgcgcg attcacgctg
       61 acgtcgccgc acgttccgcg tccaccgcat accagttgat ccgccgcgtg aagaacatca
      121 gcaacgccag cgcgccgaac aacgccgccg tgcccgccag caacgcgtaa tcctgcgact
      181 tcagcacgaa gaacagatag ccatacgtcg cgcccaaccc cgcgccgatc accagcgtgc
      241 gccgccccgt ccgcagaaac gtgtgagcat acagcgccac catcgccgtg cacaccgcgg
      301 ccgcgatgcc atacgccagt tccgtgcgca gaaactccga cagcgccaga aacccgagga
      361 aaaacaaact aagtgcgacg cccacgatcg cgtattgcag cggatgaatc cgcagcgccg
      421 ccgtcgtctc aaacaggaag aacaccgcaa aaacgagcac gaagaacagc agcccgtatt
      481 tctgcgcgcg ctcgaccaga cagtagccat ccacgggctg caggaagttc accccaaacg
      541 ccgacgccgc gatccggctg atcgcttcgc catcgtccac gagccgattc gtccagctct
      601 gcgggaaacc gcgactcaaa tgcgagctct gccactccgc cgcaaatccg cccgcgctca
      661 cgtgccgcgt gagcggcaag gacgcgccga caaacgacgg atctgcccac gccgattcca
      721 gcttcgccgt tgtcgccttg ccgatcggcg caagatcgag acgtcccgtg ccttgcaccg
      781 acaacgccac cgaaaacgtc agcttcgcgc ccggcctgac gttcgtgact ttcgccacca
      841 gcggataggg cgcacccgcc gccgtctccg ccgactcgaa ggcaaacgct tcgccccccg
      901 cgttcagagc tccgaccgaa cgaatcccgc gcagatccga aacgcccagg aacacgcgcg
      961 ccttctccca atcgatccgg tccgcctcga tcccggccgc cacaaaatcc ggtcggactt
     1021 ctccgccgag tttcagcgcg gagctgtaga ccaccgtctc ataaatcccg cgccgccgca
     1081 cctctggtgt cacctcgccc gcgatcgtca gctcctccgg cagaaaatac gcggtc
//
LOCUS       DASPLT010001591         2559 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_265587, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001591 DASPLT010000000
VERSION     DASPLT010001591.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 2559)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2559)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2559
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_265587"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1074
                     /locus_tag="VEA63_16210"
     CDS             <1..1074
                     /locus_tag="VEA63_16210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HYD85613.1"
                     /translation="SIKPDNYRYQVEQQEANLTAAKATAVQSKAQLFKAEEDFKRSEG
                     LFKTALISESDFTAARTSLEVAQANYDNALAQIRRTEGLLNQSIELLSKTTIYSPADG
                     TISSLTSEIGERVVATGSFAGTEIMRVADLNNMEVRVNVNENDVVNVKVGDKARISVD
                     AYPSRKFTGMVKEIAAAAKTQGLNTQEEVTNFEVRIRVAEKELNLRPGMSASVDIETQ
                     TVENVVAVPIQSVTVRSREGNKTLDELGADRARKERETKGEGAAAAVNEKQQRERERA
                     DREALQRVVFVRTGDTVKLTPVETGIGDTSHMEIKSGLKEGDEVVSGPFSAITRTLKD
                     GAKIRLDKPKGDAKDKEKDAKKD"
     gene            1139..1876
                     /locus_tag="VEA63_16215"
     CDS             1139..1876
                     /locus_tag="VEA63_16215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HYD85614.1"
                     /translation="MTPSPAPAHATRSVRPLGPTVIEIEGVTKLYRMGSEIIHALRGV
                     TLNIHRNEYLAIMGPSGSGKSTLMNMLGCLDTPTAGRYDFSGKNVAHMDDNELADIRN
                     REIGFVFQTFNLLPRSNALHNVELPLIYAGLPKHERIERARRALEDVGLAERMHHRPN
                     ELSGGQRQRVAIARALVTRPSIILADEPTGNLDSKTGVEIMALFEQLYEQGNTIIVVT
                     HEEDIARHARRIVRLRDGLIESDGPAQ"
     gene            1889..>2559
                     /locus_tag="VEA63_16220"
     CDS             1889..>2559
                     /locus_tag="VEA63_16220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009512112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HYD85615.1"
                     /translation="MRRFLYEFNESVRIAFTQIRANKLRSALTALGVVIGIVAVTLMG
                     TAINGIDAGVEKSFSGFGDDLLYVTKWPWRDTQDWWNYRNRRPITHEYAEKVNEWIAA
                     NPDSALKLAIPFAGRQNTIVRGEYRVNNIFTMGTSTDYARITRSEMAEGRFFSEFEAQ
                     SGANVMIVGFDVADALFPGESAVGKTVRLRGANFRVVGVPSRQGSFLGLFSWDSMVGM
                     PINTYR"
BASE COUNT          553 a          684 c          893 g          429 t
ORIGIN      
        1 agtatcaagc cggacaatta tcgctatcaa gtcgagcagc aggaggcgaa tctcacggcg
       61 gcgaaggcga cggcggtgca gagcaaggcg cagttgttca aggcggagga agatttcaaa
      121 cgcagcgaag ggctgtttaa gacggcgttg atttcggagt cggatttcac cgccgccaga
      181 acatcgctcg aggtcgcgca ggcgaactac gacaatgcgc tcgcgcagat ccgccgcacg
      241 gaagggctgc ttaaccagtc gatcgagctg ttgtcgaaga cgacgattta ctcaccggcg
      301 gatggcacga tcagctcgtt gacgagcgag atcggcgagc gcgtggtggc gacgggaagt
      361 ttcgcgggca cggagatcat gcgcgtggcg gatctcaata acatggaagt gcgcgtgaac
      421 gtgaacgaga acgacgtcgt gaacgtaaag gtcggcgaca aggctcgcat ttcggtcgac
      481 gcgtatccga gccgcaaatt caccggcatg gtgaaggaga tcgcggcggc ggccaaaacg
      541 caggggttga acacgcagga ggaagtgacg aatttcgaag taagaatccg cgtggcggaa
      601 aaagaactga acctgcggcc ggggatgagc gcgagcgtcg acatcgagac gcagaccgtc
      661 gagaacgtcg tcgcggtgcc gatccagtcc gtgacggtgc ggagtcgcga aggcaacaag
      721 acgctcgacg agctcggcgc ggaccgcgcg aggaaggaac gcgagacgaa gggcgaaggc
      781 gcggcggcgg cggtgaacga gaagcagcag cgcgaacgcg aacgtgcgga ccgcgaggcg
      841 ctgcagcgcg tggtgttcgt gcgcacgggc gacacggtga agttgacgcc ggtggaaacc
      901 gggatcggcg acacctcgca catggaaatc aaatcgggtc taaaagaagg cgacgaagtg
      961 gtgagcggcc cattcagcgc gatcacgcgg accttgaagg acggcgcgaa gatccggttg
     1021 gacaaaccga agggcgacgc gaaggacaag gagaaggacg cgaagaagga ctgatgtagc
     1081 tcggagaaag cgggcctcgc acatcgacgt gcgggggtcg ttttctccct cgattcacat
     1141 gacaccctct cctgcacccg cccacgccac gcgcagcgtg cgtccgctcg gtccgaccgt
     1201 gatcgagatc gaaggcgtga cgaaactcta ccggatgggc tccgagatca tccacgcgtt
     1261 gcgcggggtg acgttgaaca ttcatcgcaa cgagtatctc gcgatcatgg ggccttccgg
     1321 ttccggcaag tcgacgttga tgaacatgct cggctgcctg gatacgccga ccgcagggcg
     1381 ctacgatttc agcgggaaga acgtcgcgca catggacgac aacgagctcg cggacatccg
     1441 gaatcgcgag atcggtttcg tgtttcagac gttcaatttg ctgccgcgct cgaatgcgct
     1501 gcacaacgtc gagctgccgc tgatttacgc gggtctgccg aaacacgagc ggatcgaacg
     1561 cgcgcggcgg gcgctcgagg atgtcggttt ggccgaacgc atgcaccatc ggccgaacga
     1621 gctttcgggc ggtcaacggc aacgcgtagc gatcgcacgg gcgttggtga cgcggccttc
     1681 gatcatcctg gcggacgagc cgacgggaaa tctcgattcg aaaaccggcg tggagatcat
     1741 ggcgctcttc gagcagcttt acgaacaggg gaacacgatc atcgtggtga cgcacgagga
     1801 agatatcgcg cggcatgcgc gccggatcgt gcggttgcgc gatggattga tcgagtcgga
     1861 cgggccagcg cagtaacgct tttaagagat gcgccgcttt ctctacgagt ttaacgagtc
     1921 ggtgcggatc gcgttcacgc agattcgcgc gaacaagctg cgctcggcgc tgacggcgct
     1981 cggcgtggtc atcggcatcg tggcggtgac gttgatgggc acggcgatca atgggatcga
     2041 cgcgggcgtg gagaagagtt tctcgggctt cggcgacgat ctgctttatg tgacgaagtg
     2101 gccgtggcgc gatacgcagg actggtggaa ttatcggaat cgccgtccga ttacccacga
     2161 gtatgcggag aaagtgaacg agtggatcgc ggcgaatccg gactcggcgc tgaagctggc
     2221 gatccctttt gcgggccggc agaacaccat cgttcgaggc gagtatcggg tgaacaacat
     2281 cttcacgatg ggcacctcga cggactacgc gcgcatcacg cgatcggaga tggcggaggg
     2341 acgtttcttc agcgagttcg aggcgcaatc gggcgcgaac gtgatgatcg tcggcttcga
     2401 cgtcgcggat gcgttgttcc ccggcgaatc ggcggtggga aagaccgtgc ggctgcgcgg
     2461 ggcgaatttc cgcgtggtcg gcgtgccctc gcgacaaggc agttttctcg gtctgttcag
     2521 ctgggattcc atggtcggga tgccgatcaa cacctatcg
//
LOCUS       DASPLT010001592         1076 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_265612, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001592 DASPLT010000000
VERSION     DASPLT010001592.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1076)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1076)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1076
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_265612"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..416
                     /locus_tag="VEA63_16225"
     CDS             <1..416
                     /locus_tag="VEA63_16225"
                     /inference="COORDINATES: protein motif:HMM:NF014667.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HYD85616.1"
                     /translation="GRPTIVTMFFASCSYACPMLLSDLHRLREALPPAVREEAQFVLV
                     SFDSERDTPEALRAYRERTNLNAKHWTLLHGDPAAVQDLAMLLGVKYKRDARGEYAHS
                     NLITVLNAEGEIVHQQSGLQGEVVESARAITLATK"
     gene            413..652
                     /locus_tag="VEA63_16230"
     CDS             413..652
                     /locus_tag="VEA63_16230"
                     /inference="COORDINATES: protein motif:HMM:NF021520.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2249 domain-containing protein"
                     /protein_id="HYD85617.1"
                     /translation="MKSEPRKFRALDVRPLIARGEEPFHQVMTAISELQPDEGFELTA
                     PFLPSPLIERMQAAGYSVRPERHADGSWRTFFWRQ"
     gene            723..>1076
                     /locus_tag="VEA63_16235"
     CDS             723..>1076
                     /locus_tag="VEA63_16235"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HYD85618.1"
                     /translation="MRAPIDRDGRRRLFHLVPPTDVTPLRTAAIAVTLRSCQLFSGVS
                     AEDLSAIAAFSVLRPVPKGSYLFREGDPAEGFYVAQQGAINVHRVSPAGKEQTICVFR
                     PGESFAEAALATPHGY"
BASE COUNT          187 a          336 c          355 g          198 t
ORIGIN      
        1 gcggtcgacc gacgatcgtg acgatgtttt tcgcgagctg tagttatgcg tgcccgatgc
       61 tcttgagcga cttgcaccgc ctgcgggaag cgctcccgcc cgcggttcga gaggaagcgc
      121 agttcgtgct ggtaagcttc gacagcgagc gcgacacgcc cgaagcgctg cgcgcgtatc
      181 gcgagcgcac gaatctgaat gcgaagcatt ggacgctgct ccatggcgat ccggctgcgg
      241 tgcaggatct ggcgatgctc ctgggcgtga aatacaagcg cgacgctcgc ggcgaatacg
      301 ctcactcgaa tctgatcacg gtgctgaacg ccgagggcga aatcgtgcat caacaaagcg
      361 gactgcaggg tgaggttgtc gaatcggccc gcgcgattac gctggcgacg aagtgaagtc
      421 cgagccccgc aagtttcgcg cgcttgatgt gcgcccgctg atcgcccgcg gcgaggagcc
      481 gtttcatcaa gtgatgactg cgatctccga gctgcagccg gacgaaggct tcgaattaac
      541 ggcgcccttt ttgccgtcgc ccttgatcga gcgcatgcaa gccgccggct actccgtcag
      601 acccgagcgc cacgcggacg gctcgtggcg cacgtttttc tggcgacaat gaacgaagcc
      661 agcacgccct cgggagcgtg ggcgttgccg gcggaacacc gtgcagccaa ctgcggcgcg
      721 cggtgagggc gcccattgac cgcgacgggc ggagacgatt gtttcacctc gtgcctccaa
      781 ccgacgtcac tcccctccgc accgcagcca tcgcggtgac gctgcgctcg tgtcagttgt
      841 tctccggagt gtcggccgaa gatctcagtg ctatcgccgc gttctcggtg ctccgtccgg
      901 tgccgaaagg cagttatctt ttccgcgaag gcgatccggc tgaagggttt tacgtcgcgc
      961 agcagggtgc gatcaacgtc catcgcgtga gcccggccgg caaggaacag acgatctgcg
     1021 tgttccggcc gggtgagtct ttcgcggagg ccgcgctcgc gacgccgcat ggatac
//
LOCUS       DASPLT010001593         2374 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_80021, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001593 DASPLT010000000
VERSION     DASPLT010001593.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 2374)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2374)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2374
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_80021"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..440)
                     /locus_tag="VEA63_16240"
     CDS             complement(<1..440)
                     /locus_tag="VEA63_16240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD85619.1"
                     /translation="MCAVVGLAFFLSQLLEIPARSALRGYDNTFNYLWLRSAMVDGDW
                     DFRNDLETCNTLAPEHRASALGLSLTAAGRVPNKYGVGWAVVSVPFYLVADAIVATGR
                     ALGLWRLERDGYNAVYQICLQLGHLGIAWLALWLAAKAIAAWIGS"
     gene            complement(496..1206)
                     /locus_tag="VEA63_16245"
     CDS             complement(496..1206)
                     /locus_tag="VEA63_16245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HYD85620.1"
                     /translation="MSTAVKASLAPGIALRCQNIHRHLGHDEGRVHVLRGVSFEANRG
                     QVYAIVGPSGCGKSTLLYLLGLLDRQEEGDIWINGQLMSNSSDLERTAARCEHVGFVF
                     QFHFLMQEFTALENVMMPMRKLGRLSPAEMTDRAQSLLASVGLGDKTHRLGSHLSGGE
                     QQRVAIARALANQPAIILADEPTGNLDVKNSNLVFDLLTRLAKDNGQAVVLVTHNPEI
                     ASRCDSVRPMRDGLFVES"
     gene            complement(1203..>2374)
                     /locus_tag="VEA63_16250"
     CDS             complement(1203..>2374)
                     /locus_tag="VEA63_16250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HYD85621.1"
                     /translation="IVLGVGLFVVTQATTSGFEDFFIKTILGTDGAITIQDRMQDTMR
                     SMAAGGEGSQFMIRQKEGRKYISGIEEPKLVSEALAKFSNVSGTSAVLTGAVTIRSSL
                     SNESVRVFGINFDDHVRVSDLETQITHGDAAVFRSTDAGAILGSELAARSQLSVGDSF
                     ILEATGEQRRYRVSAIFKTGVSEIDRTRVFLHMSEARSLLRRPTGASFIQVNLIDKDR
                     AVEDAAHMSEVLKHLVRPWQVREETWLEVFRALRISTAITVSVFTLIAGLAMFNTLAM
                     IVLEKTKEIAILRSMGYTRKDISQIFLWQAVIVLAIGTAVGCLLGAGITYGISNYPIR
                     IRGIFATNTFIVAWSVWHYVAAITTAVTMVMIASLVPARRAALLEPGDVIRGTAQ"
BASE COUNT          495 a          807 c          669 g          403 t
ORIGIN      
        1 gaaccgatcc atgcggcgat cgccttcgcc gcgagccaga gcgccaacca cgcgatcccg
       61 agatggccga gttgaagaca gatttgatac accgcgttgt agccgtcgcg ctccaaccgc
      121 cacaacccga gtgcgcgacc cgtcgccacg atcgcatccg cgaccagata gaacggcacc
      181 gagaccaccg cccaacccac gccgtatttg ttagggacgc ggccggctgc ggtcagcgac
      241 agtccgaggg ccgaagcgcg atgctcgggc gcgagcgtgt tgcacgtctc gagatcgttg
      301 cggaagtccc agtcaccgtc gaccatcgcc gaccgcagcc aaagataatt gaacgtgttg
      361 tcgtaaccgc gcagcgcgct gcgcgccgga atctccagca actgcgacaa gaaaaacgcc
      421 agcccgacga cggcgcacac gcgcgtccaa acccgaagaa cgcccacgcg ctggcgccgc
      481 ggcgaagcgt ccgcgttacg actcgacgaa gagtccatcg cgcatcggac gcacgctgtc
      541 gcaacggctg gcgatctccg gattgtgcgt cacgaggaca accgcctggc cgttgtcctt
      601 cgcgagccgc gtaagcagat cgaaaaccag attcgagttt ttcacgtcga gatttcccgt
      661 cggctcgtcc gcgagaatga tcgccggttg attcgccagc gcacgcgcga tcgccacgcg
      721 ctgctgctcg ccgcccgaga gatgcgatcc gagccggtgc gtcttgtcgc cgagaccgac
      781 cgaggccaac agcgattgcg cgcgatccgt catctccgcc ggcgaaagcc gcccgagctt
      841 tcgcatcggc atcatcacgt tttccagtgc ggtgaactcc tgcatcagga agtgaaactg
      901 aaaaacgaaa ccgacgtgtt cgcagcgagc cgccgtgcgc tcgagatcgc tgctgttcga
      961 catcaactgg ccgttgatcc agatgtcgcc ctcttcctgc cggtcgagca acccgagcag
     1021 gtaaagcagc gtggatttcc cgcagcccga cggcccgacg atcgcataaa cctgcccgcg
     1081 attcgcctcg aacgaaacgc cgcgcaacac atgcacgcgc ccttcgtcgt gaccgaggtg
     1141 acgatgaatg ttctggcatc gcagcgcgat gcccggcgcc aaactcgctt taaccgccgt
     1201 gctcattgcg ccgtcccccg gatgacgtcg cccggctcga gcagcgccgc gcggcgcgcg
     1261 ggcacgaggc tcgcgatcat caccatcgtc accgcggtcg tgatcgccgc cacatagtgc
     1321 caaaccgacc acgcgacaat gaaggtgttg gtcgcaaaga tgccccggat gcgaatcggg
     1381 tagttggaaa ttccatacgt aatccctgcg ccgagcaggc agcccaccgc agtgccgatc
     1441 gcgagcacga ttaccgcctg ccaaagaaag atctgcgaga tatcctttcg cgtataaccc
     1501 atcgaacgta gaatcgcgat ctccttggtc ttctcgagga cgatcatcgc gagcgtgttg
     1561 aacatcgcca ggccggcaat cagcgtaaac accgacacgg tgatcgccgt cgaaattcgc
     1621 aacgcacgaa acacttcgag ccatgtttcc tcccggacct gccagggcct cacgaggtgt
     1681 ttcaagactt ccgacatatg cgcggcgtcc tccaccgcgc gatccttgtc gatcaggttg
     1741 acctggatga acgacgcgcc ggtcggccgg cgcagcagcg aacgcgcctc gctcatgtgc
     1801 aaaaaaaccc gcgtgcgatc gatctcgctc acgccggttt tgaaaattgc gctcacccga
     1861 taacgccgct gctcgccggt cgcctcgagg atgaacgagt cgccgaccga cagctgcgaa
     1921 cgtgcggcca actcgcttcc caaaatcgca ccggcatccg tgctccgaaa caccgccgca
     1981 tcgccgtgcg tgatctgggt ctccaaatcg gagacacgca cgtgatcgtc gaaattgatc
     2041 ccgaaaacgc gcaccgattc gttgctcaac gaactgcgaa tcgtcactgc accggtcagc
     2101 accgcggagg tgccggagac gttggaaaac ttcgcgagcg cttcgctcac gagcttgggt
     2161 tcctcgattc ccgagatgta tttccggccc tccttctgcc ggatcatgaa ctgcgaaccc
     2221 tcgccccctg ccgccatgct gcgcatcgtg tcctgcatgc gatcctgaat cgtgatcgca
     2281 ccgtcggtcc cgagaatcgt cttgatgaag aaatcctcga agccactcgt tgtcgcctgc
     2341 gtgacaacga aaaggccgac gccgagaacg atgc
//
LOCUS       DASPLT010001594         1868 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_243659, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001594 DASPLT010000000
VERSION     DASPLT010001594.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1868)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1868)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1868
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_243659"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..338
                     /locus_tag="VEA63_16255"
     CDS             <1..338
                     /locus_tag="VEA63_16255"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HYD85622.1"
                     /translation="EQALRTEFTASAQLEFFQSNHEGALIDKIAALAEAKFDGLVLNG
                     AAFTHTSVALRDALAGSGLRAVEVHISNIYKREEFRHRSFTAPVCVAVITGLGLEGYH
                     AAVRFLLKG"
     gene            338..880
                     /locus_tag="VEA63_16260"
     CDS             338..880
                     /locus_tag="VEA63_16260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006354 - DNA-templated transcription
                     elongation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0140673 - transcription elongation-coupled
                     chromatin remodeling [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination NusG
                     family protein"
                     /protein_id="HYD85623.1"
                     /translation="MSLDPTSPAAAVPLPVAPEGAAWLVCHTRPRCEKKFAALMAAEK
                     NAHYLPLVTSVRKYRQQTKRFTKPLFPGYVFACVPDERKSRIFQMDLLARAIGVDDER
                     RFLAQLADVKAIVASGYELSVLPLLTRGRRVKIVGGPLHGLEGFVDDPSNPRGVVLSV
                     DVLQQGLLVKLPAENLQVLP"
     gene            888..1838
                     /locus_tag="VEA63_16265"
     CDS             888..1838
                     /locus_tag="VEA63_16265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD85624.1"
                     /translation="MPFVPQPFHSARLAAGMAWCLALVAGLPAFAAPGPDPDEAVVNT
                     PPPTSPPSRNPFAAQRDALARFIDRLPELPDLGLPSFAPSGAVRLHFRPRFGDLKDED
                     YFRLPVGARIKVADRLELNAELGGYITNGLRDNAGNGLYQFRTGMKYERALDADSGWS
                     AGFEWNTPLSHPPIEMIDGLRHTLPYVTFTRTVMPRYGLVGFATLGADLIDRTSLPEN
                     YAENQLRSNSLILTLGVAREWRRMHVILRVFDANTAPLSGESENVFGLRPSIGVPILR
                     RDDGTPRATVTFEGRTIWGPDGFETGISTRVRVDLRYRRK"
BASE COUNT          298 a          700 c          518 g          352 t
ORIGIN      
        1 tcgagcaagc gctgcgcacc gagttcaccg cttcggcgca gctcgaattt ttccaatcga
       61 atcacgaggg tgcgctcatc gacaaaatcg cggcgctcgc cgaggcgaag ttcgacggcc
      121 tcgtgctcaa cggcgccgcc ttcacgcaca ccagcgtcgc gctccgcgat gcgctcgccg
      181 gctccggcct ccgcgccgtc gaagttcaca tttccaatat ctacaagcgc gaggaattcc
      241 gtcaccgctc gttcaccgcg cccgtgtgcg tcgccgtgat cacgggcctc ggtctcgaag
      301 gttatcacgc cgccgtgcgt tttctgctca agggctgatg tcgctcgacc ccacgtcccc
      361 tgctgcggcc gttcctcttc ccgtcgcccc ggaaggcgcg gcgtggctcg tctgccacac
      421 gcgtccgcgc tgcgaaaaaa aattcgccgc gctcatggcc gccgagaaaa acgcgcacta
      481 cctgccactt gtcaccagtg tgcgcaaata ccgccagcaa acgaagcggt tcaccaagcc
      541 gctctttcct ggctacgtgt tcgcctgcgt gccggacgaa cggaagtcgc gcattttcca
      601 gatggacctc cttgcgcgcg cgatcggcgt cgacgacgaa cgtcgctttc tcgcgcaact
      661 cgccgacgtg aaagcgatcg tcgcctccgg ctacgagctt tccgttcttc cgcttctcac
      721 gcgcggccgc cgcgtgaaaa tcgtcggcgg ccccctccac ggcttggagg gtttcgtcga
      781 cgatccgtcg aaccctcgcg gcgtcgtgct gtcggttgat gtgctccagc agggtctgct
      841 cgtaaagctc cccgcagaaa acctgcaggt gcttccctag cccacacatg cctttcgttc
      901 cccagccgtt tcattccgcg cgcctcgccg ccggaatggc ctggtgcctc gcgctcgtcg
      961 ccggactccc cgcgttcgcc gcgcccggcc ccgatcccga cgaggccgtg gtcaacaccc
     1021 cgccaccaac gtcgccgccg tcgcggaatc ccttcgcggc gcaacgcgac gccctcgcgc
     1081 gcttcatcga tcgcctgccc gagcttcccg atctcggcct ccccagtttc gcaccctccg
     1141 gcgcggtgcg tcttcacttc cgtccgcgct tcggcgattt gaaggacgaa gattattttc
     1201 ggctgcccgt tggcgctcgg atcaaggtcg ccgatcgcct cgaactaaac gccgagttgg
     1261 gtggctacat cacgaacggc ctgcgcgaca acgccggcaa cggcctctac caatttcgca
     1321 ccggcatgaa atacgagcgc gccctcgatg ccgactccgg ctggagcgcg ggcttcgagt
     1381 ggaacacccc gctcagccac ccaccgatcg agatgatcga cggccttcgc cacacgctgc
     1441 cctacgtcac gttcacgcgc accgtgatgc ctcgctacgg acttgtcggt ttcgcgacgc
     1501 tcggcgccga cctgatcgat cgcacgtcgt tgccggaaaa ttacgctgag aaccaacttc
     1561 gctcgaactc gctcattctc acgctcggcg tcgcgcgcga atggcgccgg atgcacgtca
     1621 tcctgcgcgt gttcgacgcc aataccgctc cgctcagcgg cgaatccgaa aacgttttcg
     1681 gtctccgccc aagcatcggt gtgcccattc ttcgacgcga cgacggcact ccgcgcgcca
     1741 ccgtcacgtt cgaaggccgc accatctggg gccccgatgg tttcgagacc ggcatctcca
     1801 cgcgcgtccg cgtcgacctc cgttaccgcc gaaaatagcc ggtagggacg cctctccgag
     1861 gcgtccgc
//
LOCUS       DASPLT010001595         1465 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_100220, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001595 DASPLT010000000
VERSION     DASPLT010001595.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1465)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1465)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1465
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_100220"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..242)
                     /locus_tag="VEA63_16270"
     CDS             complement(<1..242)
                     /locus_tag="VEA63_16270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD85625.1"
                     /translation="MSSRRTEFSRRAARWLLPAAVLAFTPKCLLCVAAYVGLGAALGL
                     ATPELCGGAANASTTWASSLAWLGLICGLSAFGFRA"
     gene            complement(223..966)
                     /locus_tag="VEA63_16275"
     CDS             complement(223..966)
                     /locus_tag="VEA63_16275"
                     /inference="COORDINATES: protein motif:HMM:NF017771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HYD85626.1"
                     /translation="MKTHSFENPTVVSSEKWLAARRELLREEKKFTRLRDQLAARRRE
                     LPWVKVDKSYEFASSTGRVALANLFEDRSQLVVYHFMLGPGWGEGCKSCAYVADHLAP
                     AVVHLHARDVAFAAISRAPLAEIASFKERMGWAFNWVSSHDNDFNYDYRVSFTPEEFA
                     KGKVDYNYDMTEFPSEEAPGLSVFARAKNGEIYHTYSTYARGLDVLIGTYTLLDLVPK
                     GRDEGDDTTMSWVRYHDSYDQPADVVATH"
     gene            complement(1113..>1465)
                     /locus_tag="VEA63_16280"
     CDS             complement(1113..>1465)
                     /locus_tag="VEA63_16280"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HYD85627.1"
                     /translation="APDETPPAKYAHVQDPGVTFEGRVLRCEPPRLLAYTFGSEDSEV
                     TFELTPEGQQVLLVLTHRTRGDEERAELTNYASGWHTHLALLVALLEGGPRPKFWAMH
                     AKLLADYEKVRASA"
BASE COUNT          273 a          422 c          503 g          267 t
ORIGIN      
        1 ctcgcgcgaa atccaaacgc gctgagtccg cagatgaggc cgagccaagc gagcgatgag
       61 gcccacgtcg ttgaggcgtt cgcggcgccg ccgcagagct ccggtgtcgc gagcccgagc
      121 gctgcgccga ggccgacgta ggccgcgacg caaagcaggc atttcggcgt gaacgcgagc
      181 accgccgccg gcagcagcca gcgggcggct cggcgggaaa actcagtgcg tcgcgacgac
      241 atcggcgggc tggtcgtagc tgtcgtgata acgaacccag ctcatcgtcg tgtcatcgcc
      301 ttcgtcgcgg cctttgggca cgaggtcgag cagggtgtag gtgccgatca acacatcgag
      361 accgcgcgca taggttgaat acgtgtggta gatctcgccg tttttcgcgc gggcgaagac
      421 gctgagcccg ggcgcctcct ccgaaggaaa ctccgtcatg tcgtagttgt agtcgacttt
      481 gcctttcgcg aactcctcgg gagtgaagga cacgcggtag tcgtagttga aatcgttgtc
      541 gtgagacgaa acccagttga atgcccaacc catccgctcc ttgaacgacg cgatctcggc
      601 gagcggcgcg cgggagattg cggcgaaagc aacgtcgcgt gcatgcaggt gcacgacggc
      661 gggcgcgaga tgatccgcca cgtaggcgca gctcttgcag ccttcacccc agccgggccc
      721 gagcatgaaa tgatagacca cgagctggct gcggtcttcg aataaattgg cgagcgccac
      781 gcggccggtg gacgaggcga attcataaga tttgtccacc ttcacccacg gcagctcgcg
      841 gcgccgcgcg gcgagctggt cgcggagtcg cgtgaacttt ttttcttcgc gcagcaattc
      901 gcggcgggcc gcgagccatt tttcggagga gacgacggtt ggattttcga acgaatgggt
      961 tttcatggga ggaacgattt tggattgagc taacggttga tgagcgggca agggagcgag
     1021 gcgtccgggc aaggcagtgg ccgtggttca atcgaacgcg attgggagcg gaacgttcgc
     1081 gtggcgtccg ctccgaatat tcggacgagt tattacgcgg aggctcggac tttttcgtaa
     1141 tcggccagaa gtttcgcgtg cattgcccaa aacttggggc gcggaccccc ttcgagcagc
     1201 gccacaagaa gggcgaggtg cgtgtgccag cccgaggcgt agttggtgag ttcggcgcgt
     1261 tcttcgtcgc cgcgggtgcg atgcgtgagg acgagcaaga cttgctgacc ttcgggcgtc
     1321 agctcgaacg tcacttccga atcctcgctg ccaaacgtgt aggcgagcaa ccgcggcggt
     1381 tcacaccgga gcacgcgtcc ctcgaaggtg acgccgggat cctgcacgtg ggcgtatttc
     1441 gccggcggcg tctcatcggg cgcga
//
LOCUS       DASPLT010001596         1169 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_223888, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001596 DASPLT010000000
VERSION     DASPLT010001596.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1169)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1169)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1169
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_223888"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..725
                     /locus_tag="VEA63_16285"
     CDS             <1..725
                     /locus_tag="VEA63_16285"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HYD85628.1"
                     /translation="PSAINHHWTKAMTAGADGRFLYVGIGSNSNITERGMTAEVNRAE
                     VWQIDAETGMHKPYATGLRNPTALAIQPGTGVLWAAVNERDELGPDLVPDYLTHVQEG
                     GFYGWPYAYWGPNPDPRVKPQKPEKVKATIKPDYALGSHVAALGLAFSVPAMGKDYAD
                     GVFVGEHGSWNRNPPVGYKVVFVRFRDGKPVGEPIDFVTGFREEDGTTYGRPVGVSVD
                     PRGALIVCDDLANIVWRVTPTR"
     gene            739..>1169
                     /locus_tag="VEA63_16290"
     CDS             739..>1169
                     /locus_tag="VEA63_16290"
                     /EC_number="1.13.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class III extradiol ring-cleavage dioxygenase"
                     /protein_id="HYD85629.1"
                     /translation="MPALFIGHGSPMNTLERNDFTEAWRTFGKQLPQPRALLVVSAHW
                     YIGATAVTAMPKPRTIHDFYGFPAALFDFEYPAPGAPDVAGEIAEAVKPTWCGLDVDQ
                     WGLDHGTWSVLAHVYPEADVPVLQLSLNALKPMDYHLALGA"
BASE COUNT          205 a          378 c          392 g          194 t
ORIGIN      
        1 tgccgtcggc gatcaaccat cactggacca aggcgatgac cgccggcgcg gacggccgct
       61 ttctgtatgt cggcatcggc tcgaacagca acatcacgga acgcggcatg acagccgagg
      121 tgaatcgcgc ggaggtgtgg cagatcgatg cggagaccgg catgcacaag ccctacgcga
      181 ccggcttgcg gaatcccact gcgctcgcca tccagccagg cacgggcgtg ctgtgggcag
      241 cggtgaatga acgcgacgag ctcggcccgg atctcgtgcc cgactatctc acgcacgtgc
      301 aggaaggcgg cttctacggc tggccctatg cctactgggg tccgaatccc gacccgcgcg
      361 tgaaaccgca gaaacccgag aaggtgaagg cgaccatcaa gcccgactac gcgctcggct
      421 cgcacgtcgc ggccctcggg ctcgcgtttt cggtgccggc aatggggaag gactacgccg
      481 acggtgtgtt cgtcggcgag cacgggagct ggaaccgcaa tccgccggtc ggctacaagg
      541 tcgtgtttgt gcgctttcgc gacggcaagc cggtcggcga gccgatcgat ttcgtcacgg
      601 gcttccgcga ggaagacggc acgacgtacg gccggcccgt cggcgtgagc gtggacccgc
      661 gcggtgcgct catcgtttgc gacgatctgg cgaacatcgt gtggcgcgta acgccgacgc
      721 gttgagacta tggcccgcat gcccgctttg ttcatcggcc atgggagtcc gatgaacacg
      781 ctcgagcgca acgacttcac cgaagcgtgg cgcacgttcg gcaagcaact cccgcaaccg
      841 cgagcgttgt tggtcgtatc cgcgcattgg tacatcggcg cgacggcggt cactgcgatg
      901 ccgaagccgc gcaccatcca cgacttctac ggctttcctg cggcgctgtt cgatttcgaa
      961 tatccggcgc ccggcgcacc ggatgttgcg ggcgagatcg ccgaggccgt caagccgacg
     1021 tggtgcggcc tcgatgtcga tcagtggggc ctcgaccacg gcacatggag cgtgctcgcg
     1081 cacgtctatc cggaagcgga cgtgccggtg ctgcaactgt cgctcaacgc gctgaagccg
     1141 atggactatc acctggcgct cggcgcgca
//
LOCUS       DASPLT010001597         2183 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_223893, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001597 DASPLT010000000
VERSION     DASPLT010001597.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 2183)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2183)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2183
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_223893"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2183
                     /locus_tag="VEA63_16295"
     CDS             <1..>2183
                     /locus_tag="VEA63_16295"
                     /inference="COORDINATES: protein motif:HMM:NF017263.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alginate lyase family protein"
                     /protein_id="HYD85630.1"
                     /translation="FTSTASAQFTHPGGLHNQADLDRMRSQVAAGAQPWLAGWNALIA
                     DPDAQSTYTPAPNANMGANRQRASRDAHAAYLNAIRGYVANSTTHTDRAIQICNAWSA
                     AVNQVPTGTDIPGLSGIPIFEFALVGEILRIHVPSRWATADFNRFKNMMLTYLYPVCN
                     DFLVNHNGSCISNYWTNWDAANIGAVMVIGVLCDDQAKFNQARNYFQSGAGMGSIMNA
                     VPFLHASGLGQWQESGRDQPHAMLGIGLLGAVCEVAWKQGIDLYGYSSNRLLAGAEYT
                     ALYNLWKSVPYTFYNNCQPANNKWVSINGRGQIHDRPMWELIYNHYVVRQGLNAPNTA
                     AMAKIARPERGSADHFGYGTLTFTLAASTYPPSPTPLAPSGVNATAGVGRVFLKWSPA
                     AGDTAQGYEVRRATNSGGPYTSVASWNNSPRCEYIDTSVSNGTTYHYVVASRNQSGTS
                     GNSAQASATPLATGGLPSGWIRTSIGTVSGAAAGFANVSGGTYVVAGSGTIGGTADAI
                     SFVNRTVTGDATLTARISSLGGTVLKSGVMMRESTAANARTVSMTLGDAGWRFARMGA
                     RTTAGGSMGFTQGNAYTWVPAWFRISRVGNVFTASESSDGVNWFTVGSTTITMPATYV
                     IGLAISSGNTSVNNTTFDNVTVSGGGSSAFPPAGTYKLISRTSGRRLDNMGSTADGAD
                     VAQWQDSASNNQRWVLSYVSADVVKLQCVTGGKYLDGIGRTANGS"
BASE COUNT          410 a          713 c          691 g          369 t
ORIGIN      
        1 cttcacctcg actgcttccg cccagttcac ccatcccggc ggccttcaca atcaggccga
       61 tctcgatcgc atgcgctcgc aggtcgcggc cggcgcgcag ccgtggctcg ccggttggaa
      121 tgcgcttatc gccgaccccg acgcgcagtc gacttatacg ccggcgccca acgccaacat
      181 gggcgccaat cgccagcgcg cctcgcgcga tgcgcatgcg gcctatctca acgcgatccg
      241 cggctacgtc gcgaacagca ccacgcacac cgaccgtgcg attcagatct gcaacgcctg
      301 gtccgcggcg gtgaaccagg tgccgacggg caccgacatc ccgggcctct cgggcattcc
      361 gattttcgag ttcgcgctcg tcggtgagat cctgcgcatc cacgtgcctt cacgctgggc
      421 gacggcggat ttcaaccgct tcaagaacat gatgctcacg tatctgtatc cagtttgtaa
      481 tgactttctg gtgaatcaca acggctcgtg catctcgaac tactggacca actgggatgc
      541 cgccaacatc ggcgctgtca tggttatcgg cgtgctgtgc gacgaccagg cgaagttcaa
      601 tcaggcgcgc aactatttcc aaagcggcgc cggcatgggc agcatcatga acgccgtgcc
      661 gttcctgcac gcaagcggac ttggtcagtg gcaggagagc ggccgcgatc agccgcacgc
      721 gatgctcggc atcgggctgc tcggcgcggt ttgcgaagtc gcgtggaagc agggcatcga
      781 tctctacggt tactcgagca accggctgct cgccggcgcc gaatacaccg cactctacaa
      841 tctctggaaa tccgttccct acacgttcta caacaattgc cagccggcga acaacaagtg
      901 ggtctcgatc aacggtcgtg gacaaattca cgaccggccg atgtgggagt tgatctacaa
      961 tcactacgtc gtgcgccagg gtctcaacgc gccgaatacg gcggcgatgg cgaagatcgc
     1021 ccgccccgaa cgcggcagcg ccgatcattt cggctacggc acgctgacgt tcacgctcgc
     1081 ggcctcgacg tatccgcctt cgccgacgcc gctcgccccg agcggcgtga acgcgacggc
     1141 gggcgtggga cgcgttttcc taaaatggtc gcccgccgcc ggcgataccg cgcagggcta
     1201 cgaggtgcgg cgcgcgacga acagtggcgg cccgtatacg agtgtcgcga gttggaacaa
     1261 cagcccgcgc tgcgagtata tcgataccag cgtcagtaac ggcacaacgt atcactatgt
     1321 ggtcgcctcg cgaaatcagt cgggcaccag cggcaactcc gcgcaggcca gcgccacgcc
     1381 gctcgcgacg ggcgggcttc cttccggttg gattcgcacc agcatcggaa cggtaagcgg
     1441 agctgcagcc ggcttcgcca acgtaagcgg cggcacctac gtcgtcgctg gctccggcac
     1501 gattggcggc acggcggacg cgatcagctt cgtgaatcgc accgtcaccg gtgacgccac
     1561 gctcacggcg cgcatttcga gcctcggtgg cacggtgctg aagtccggcg tgatgatgcg
     1621 cgagtccacc gccgcgaatg cgcgcacggt gagcatgacg ctcggcgacg ccggttggcg
     1681 gttcgcgcga atgggcgcgc gcaccaccgc cggtggcagc atgggcttca cgcagggcaa
     1741 cgcctacacg tgggtgccgg cgtggttcag aatcagccgc gtcggcaatg tgttcaccgc
     1801 gtccgaatca tccgacggcg tcaactggtt caccgtggga tcgacgacga tcacgatgcc
     1861 cgccacatat gtgatcgggt tggcgatcag ctccgggaac acgtcggtga acaatacgac
     1921 gttcgacaac gtcacggtga gcggtggcgg cagcagcgcg ttcccgccgg ccggaaccta
     1981 caaactgatc agtcgcacga gtgggcggcg gttggacaac atgggctcga cggctgacgg
     2041 cgcagatgtc gcgcagtggc aggacagcgc gagcaacaat caacggtggg tgctctcgta
     2101 tgtcagtgcg gatgtggtga agctccagtg cgtgaccggc ggcaaatacc tcgatggcat
     2161 cgggcgaacc gccaacggct cga
//
LOCUS       DASPLT010001598         1092 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_306435, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001598 DASPLT010000000
VERSION     DASPLT010001598.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1092)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1092)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1092
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_306435"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1092)
                     /locus_tag="VEA63_16300"
     CDS             complement(<1..>1092)
                     /locus_tag="VEA63_16300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD85631.1"
                     /translation="AAFQAATADHIVVRDCRFARLGGTGVMFTQGGNDNVIEGNSFFD
                     LAAGGIEFGEDAAQPKSARLFPRRNRIANNFIARIGEDYFGSVAILGYYTDALHITHN
                     EIAAIPYTAISQGWGWEKPRSPDDARANRITHNRIVNYMRRLDDGGGIYTTGRQAGSE
                     IAHNYLARMLPPNPHTKAGGALYPDQCTEGFHLHHNVVEDAVRWLNIWNPNIRHNRVD
                     TNYANTSAQRNDGTDNHVEPVHLAANGRWPKPAQAIIAAAGLEPEFAHALQIAAPSDV
                     IVESTGLNFQTIAGTWTTTDLPADRYSAYGRQSNDADATARWTPILPRGGRYQVSVWH
                     AAGAAPARYSIRHRVGTAEVETKTTAEGGW"
BASE COUNT          162 a          305 c          406 g          219 t
ORIGIN      
        1 ccaaccgccc tcggcggtgg ttttggtttc gacctcggcg gttcccacgc gatggcggat
       61 cgaataacga gccggcgccg ctcccgcggc gtgccagacg ctcacttgat agcgaccgcc
      121 tctcggcagg atcggcgtcc aacgcgcggt ggcgtcggcg tcgttggatt gccggccata
      181 cgcgctgtag cggtcggcgg gaagatcggt cgtggtccac gtgcccgcga tggtttggaa
      241 attcaatccg gtgctctcga cgatcacgtc ggacggagcg gcgatctgga gcgcgtgggc
      301 gaactccggt tcgaggccgg cggcggcgat gatcgcttgc gcgggcttgg gccagcggcc
      361 gttggcggcg agatgaacgg gctcgacgtg gttgtcggtg ccgtcgttgc gttgggcgga
      421 ggtgttcgcg tagttcgtgt cgacgcggtt gtggcggatg ttgggattcc agatgttcag
      481 ccagcgcacg gcgtcttcga cgacgttgtg atggagatga aaaccttcgg tgcactgatc
      541 cggatacaac gcgccgccgg ctttcgtgtg cgggttgggc gggagcatgc gtgcgaggta
      601 gttgtgcgcg atttccgaac cggcctggcg gccggtcgtg taaatgccgc cgccgtcgtc
      661 gaggcgccgc atgtagttca cgatgcggtt atgagtgatc cgattcgcgc gcgcgtcgtc
      721 ggggctgcgc ggtttttccc aaccccagcc ctggctgatg gcggtgtagg gaatggcggc
      781 gatctcgttg tgggtgatgt gcagcgcgtc ggtgtagtag ccgaggatcg cgacggagcc
      841 gaaataatct tcgccgatgc gcgcgatgaa attgttcgcg atgcgattgc ggcgagggaa
      901 gagacgcgcg ctcttcggct gggcggcgtc ttcgccgaac tcgataccgc cggcggcaag
      961 atcgaaaaac gaattgccct cgatgacgtt gtcgttgccg ccttgcgtga acatcacgcc
     1021 ggtgccgccg agacgcgcga agcggcagtc gcgcacaacg atgtggtcgg cggtcgcggc
     1081 ttgaaatgcc gc
//
LOCUS       DASPLT010001599         2975 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_80043, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001599 DASPLT010000000
VERSION     DASPLT010001599.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 2975)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2975)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2975
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_80043"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1284)
                     /gene="murJ"
                     /locus_tag="VEA63_16305"
     CDS             complement(<1..1284)
                     /gene="murJ"
                     /locus_tag="VEA63_16305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HYD85632.1"
                     /translation="MAPTTWRVSKKLKNIGVVSLLTLVSRVLGLMRDQFGAAIFGASV
                     LNSAFITAFSLPNLFRRLLGEGALTAAFVPTLQDELRDNGRAGAFRLLNQVGSWLLLV
                     CGGLAVVAMVGFSQSRLLPGHEPRWYLAADLTALLFPYLVMICVAAAFNAALNVFERF
                     TEPVLSPIWLNLAMISFLAGAWYVTEDEMARMHWQCAGVLVGGLAQMLVPAGVLIREG
                     WRPRFDFKWTRRVQEIAVLMAPGLFGTAIYQINVYVSRLFAFSIDDAAATHIFYANRL
                     MELPIGVFAIAVATVIYPHIAKHAAEKNFAGMAADYRNGVRLILMINVPAALGLVVLS
                     EPIVRLLFQHGNFLAEDTRSMAMLLALFAIGMPFYSVSSLTTRGFYATKDTVTPVKIA
                     TLSFVINVGLSWSLKGPLGAPGLVLASTVAVVVQTL"
     gene            complement(1389..2057)
                     /locus_tag="VEA63_16310"
     CDS             complement(1389..2057)
                     /locus_tag="VEA63_16310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD85633.1"
                     /translation="MNKKIQRELYGPSWTEVILGALLSLLLGVALAAAFLVFKPVTIA
                     RELPAEPKEGVVYYLEGSRNAAKARLGAGKEQVFLQGGTIELSEDELNAWAGENAPAA
                     TQPAPSGNASGGPAVASSAPNFRIRDGVLQIAVPVDIKVLGLNQRVILQARGGFEKDG
                     ETWMFEPSELLVGSCPLQRVPFVQGLVMKRVVAAVNMPDEVLTAWRGLSNVVVDGSTL
                     RLEG"
     gene            complement(2121..2417)
                     /locus_tag="VEA63_16315"
     CDS             complement(2121..2417)
                     /locus_tag="VEA63_16315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HYD85634.1"
                     /translation="MPTYEYSCPKCGHAFEQFQSMNDAPLTQCPKCKKKGVKRLIGGG
                     AGLIFKGSGFYITDYKNQKSPEKKGGESGGGEKAAPAKTETKAESKPAKSKPAN"
     gene            2558..>2975
                     /locus_tag="VEA63_16320"
     CDS             2558..>2975
                     /locus_tag="VEA63_16320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HYD85635.1"
                     /translation="MDFAAEHRRLVDHVERGLDQHLPPATTRPARLHEAMRYSLQAGG
                     KRLRPVLVLATAARFGAADDRALPAAVAIECLHTYSLIHDDLPCMDNDDLRRGRPTAH
                     KAFDEATALLAGDALLTHAFALLASNYAAQPALAAAL"
BASE COUNT          569 a         1113 c          782 g          511 t
ORIGIN      
        1 cagcgtctgc accacgaccg ccaccgtgct cgcaagcacg agtcccggcg cgccgagcgg
       61 acctttcaag ctccagctca agcccacgtt gatcacgaaa ctcagcgtcg cgatcttcac
      121 cggcgtcact gtgtccttcg tcgcataaaa cccccgcgtt gtgaggctcg acaccgagta
      181 gaacggcata ccgatcgcga acagcgcgag cagcatcgcc atgctgcgcg tatcctccgc
      241 gagaaaattc ccgtgctgaa acaacagccg cacgatcggc tcgctcagca ccactagccc
      301 cagcgccgcc ggcacgttga tcatcagaat caggcgcacg ccatttcgat agtccgccgc
      361 catccccgcg aaattcttct ccgccgcgtg cttcgcgata tgcggataaa tcaccgtcgc
      421 caccgcgatc gcgaacacgc cgatcggcag ctccatcagc cggttcgcat aaaaaatatg
      481 cgtcgccgcc gcgtcgtcga tcgagaacgc gaacagccgg gaaacgtaga cgttgatctg
      541 atagatcgcc gtcccgaaca accccggcgc catcaacacc gcgatctcct gcacacgtcg
      601 cgtccacttg aaatcgaacc gcggccgcca gccttcgcgt atcaacactc ccgccggcac
      661 caacatctgc gccagcccgc ccacgagcac gcccgcacat tgccagtgca tgcgcgccat
      721 ttcgtcctcc gtgacatacc acgcgccggc cagaaacgaa atcatcgcga ggttcagcca
      781 gatcggcgac agcaccggct cggtgaaccg ctcgaagaca ttcagcgctg cgttgaacgc
      841 cgccgccacg cagatcatca ccagatacgg aaacagcagc gccgtgaggt ccgccgcgag
      901 ataccagcgt ggctcgtgtc ccggcagcag ccgcgactgg ctgaaaccca ccatcgcgac
      961 aaccgcaagc ccgccgcaca ccagcagcag ccagcttcca acctgattca acaaacgaaa
     1021 cgcgcccgcg cggccgttgt cgcgcaattc gtcctgcaat gtcggcacga acgccgccgt
     1081 caacgcgccc tcgccgagta gccgccgaaa cagattcggc aaactaaacg ccgtgataaa
     1141 cgccgagttc agcacgctcg caccgaagat cgccgcgccg aactggtcac gcatcagccc
     1201 gagcacgcgc gacaccaggg tcagcagcga aacgacgccg atgttcttca gcttcttcga
     1261 cacgcgccaa gttgtcggtg ccacgcctca gtcctgcaag accttacccg cccgcccgtc
     1321 catggaccac cctgcagggc agggtctccc gatccccgcc gcgctccgct catccgcatg
     1381 ctccccgctc acccttccag ccgcagcgtc gaaccatcga cgactacgtt ggacagcccg
     1441 cgccacgccg tcagaacttc gtcgggcatg ttcaccgccg cgacgacacg tttcatcaca
     1501 agcccctgaa cgaacggcac ccgctgcaac ggacacgagc ccaccagcaa ttcgctcggc
     1561 tcgaacatcc atgtctcgcc gtctttctcg aagccgcccc gcgcctgcag aataacccgc
     1621 tggttgagcc cgagcacctt gatgtccacc ggaacggcaa tctgcaagac accatcacgt
     1681 atccgaaaat tgggtgcact cgaagccacc gccggcccgc ccgacgcatt gccgcttggc
     1741 gccggctgcg tcgccgccgg cgcattctcg ccggcccatg cgttcaactc gtcctcgctc
     1801 aattcgatgg ttccgccctg cagaaacacc tgctccttcc cggcgccgag ccgggccttt
     1861 gcagcgttcc gcgagccctc gagataataa acgacgcctt ccttcggctc cgcaggcagc
     1921 tccctcgcaa tcgtcacggg cttgaaaacg agaaaagccg ccgccagggc cacgccaagc
     1981 aaaaggctca ggagtgcgcc gagaatgacc tccgtccagc tcggcccgta gagttcgcgt
     2041 tgaattttct tattcatggt tgggagcagg cgggcttgga aaacgcaatc agcgtggcga
     2101 ctcggccgcc gcgcggcgcc tcaatttgcg ggcttcgatt tcgcgggttt cgactccgcc
     2161 ttcgtctcgg tttttgccgg cgcggctttt tcgccgccgc cggattcgcc gccctttttc
     2221 tcgggcgatt tctgattctt gtagtcggtg atgtagaagc ccgaaccttt gaaaatcagg
     2281 ccggcgcctc cgccgatcag ccgcttcacg cccttcttct tgcactttgg gcactgcgtg
     2341 agcggtgcat cgttcatcga ctgaaactgt tcgaaggcgt gaccgcactt cgggcaggaa
     2401 tactcgtagg tcggcatagt gagaacgcgc gaatttatga aagctctctc cacgttggcg
     2461 agttcgtttc cacgctttct ctcagccttt gaccgctccg cgcgccgtga cacgctccgc
     2521 ttgcgccgaa gcgccttttc gcgcacgcac ttgcgggatg gattttgccg ccgaacaccg
     2581 ccgcctcgtc gatcacgtcg aacgcggcct cgaccaacat cttccccccg cgacgacgcg
     2641 ccccgcccgc ttgcacgaag cgatgcgcta cagcttgcag gccggcggca agcggctgcg
     2701 ccctgtgctc gtgctcgcca ccgccgcccg cttcggcgct gccgacgacc gcgcgctgcc
     2761 cgccgctgtc gcgatcgaat gcctgcacac gtattcgtta atccatgacg atctgccgtg
     2821 catggacaat gacgacctcc gccgcggccg ccccactgcc cacaaagcct tcgacgaagc
     2881 caccgcgctc ctcgccggcg acgccctgct cacgcacgcg ttcgctctgc tcgcgtcgaa
     2941 ttacgccgcg caaccggcgc tcgctgccgc actcg
//
LOCUS       DASPLT010001600         1527 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_243693, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001600 DASPLT010000000
VERSION     DASPLT010001600.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1527)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1527)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1527
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_243693"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(204..1034)
                     /locus_tag="VEA63_16325"
     CDS             complement(204..1034)
                     /locus_tag="VEA63_16325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD85636.1"
                     /translation="MGPTILNADEVAAQLSQSIVTLAKLTGFSERTVTLAECGAILKA
                     CVGKTKVAAPGEIRSRVRTKVFRHVGVTSGQQSVTINTGARGTYGRVWSYVKNPANPR
                     PASRRDWLLVGQLSDDGQRFTPAKYRVRRWSDVIDLTQEVARELKKAVPPALASAGLA
                     RQSWVQIADDLGIILEDIPGGSVSAAAIAKARKAIASNGRTYTNGIGTMAYEENKSFL
                     AKLVNQLPYNTRAKLDLTLASVMAGRARYFAENVNRQVLASHAATVRAYPWMKLLGAG
                     "
     gene            complement(1037..>1527)
                     /locus_tag="VEA63_16330"
     CDS             complement(1037..>1527)
                     /locus_tag="VEA63_16330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD85637.1"
                     /translation="TMPFISEYDYYEGTLALTVATQRPDAAPSAVLVSAALHEEWASR
                     ARCLLREYRNNLGAYLPYYRVCKLMPAGSVREIENNYDLLRMQFSVGFTLLQSAWTAD
                     ETLVFAFERNIEKAVRDLFEAHDLPHLFIPGDTPDLGDSYIDVILSLGAATNQGAWQV
                     PS"
BASE COUNT          274 a          421 c          553 g          279 t
ORIGIN      
        1 tcgagcaacg cccattgatt gctcgtgttg aggtaaccga cctggcccgc ggtgaacgtg
       61 gcgccgaact tgtattggga gaatatcttc gctccagcgg aggcgacgac gttggcggcg
      121 gtgacggtga tatcggccat gggagtttag gagaaagctg aaacgacgcc gggaggcggg
      181 tggttacacg ggagcgatgg atgtcaacct gcgccgagaa gcttcatcca cggatacgcg
      241 cgcacggtcg cagcgtggga ggcgagcacc tgacggttca cgttttccgc aaagtagcgc
      301 gcgcggccgg ccatgacgga ggcgagcgtg agatcgagtt tcgcccgcgt gttgtagggg
      361 agctggttta cgagcttcgc gaggaagctt ttgttttcct cataggccat ggtgccgatg
      421 ccgttggtgt aggtgcggcc gttcgacgcg atggccttgc gggctttcgc gatcgccgcc
      481 gccgagacgc tgccaccggg gatatcctcg aggatgatgc cgaggtcgtc ggcgatctgc
      541 acccacgact ggcgcgcgag gccggcggag gcgagagcgg gaggcaccgc tttcttgagc
      601 tcgcgagcga cctcctgggt gagatcgatg acatcgctcc agcggcgcac gcgatatttt
      661 gccggcgtga atcgctgacc atcgtccgac aactgaccca cgagcagcca atcccggcgc
      721 gacgccgggc gagggttggc cgggtttttc acgtagctcc acacgcggcc ataggtgccg
      781 cgagcaccgg tgttgatggt gacgctctgc tggccggagg tgacgccgac atggcgaaac
      841 accttcgtgc gcacgcgcga ccgaatctcc cccggagcgg ccaccttggt cttgccgacg
      901 caggctttga gaatcgctcc gcactcggca agggtgacgg tgcgctcgct gaagccggtg
      961 agcttcgcca gcgtgacgat cgactgcgag agctgggcgg cgacctcgtc ggcgttgagg
     1021 atcgtcgggc ccatgatcag gagggcacct gccaggcgcc ttggttggtg gcggcaccga
     1081 gcgagaggat cacgtcaatg taggaatcgc cgaggtcggg ggtgtcgccg gggatgaaga
     1141 gatgcgggag gtcgtgggct tcgaaaagat cgcgcaccgc tttctcgata ttgcgctcga
     1201 aagcgaagac gagcgtctcg tcggctgtcc aggcgctctg cagcagcgtg aagccgacgc
     1261 tgaactgcat gcgcaggaga tcgtagttgt tttcgatttc gcgaacggaa ccggcgggca
     1321 tgagtttgca cacgcggtaa taggggaggt aggcgccgag gttgtttctg tattcacgaa
     1381 gcaagcaccg agcacgggac gcccattcct cgtggagcgc ggcagagacg agcacagcgg
     1441 atggcgccgc atcggggcgc tgggtggcaa ccgtgagcgc gagcgtgcct tcgtagtaat
     1501 cgtattcgct gatgaagggc atggtgg
//
LOCUS       DASPLT010001601         1106 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_100283, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001601 DASPLT010000000
VERSION     DASPLT010001601.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1106)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1106)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1106
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_100283"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..474)
                     /locus_tag="VEA63_16335"
     CDS             complement(<1..474)
                     /locus_tag="VEA63_16335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009513910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HYD85638.1"
                     /translation="MEVVFLGTGTSQGVPMIACDCAVCTSSDPRNKRTRASIHVVMDG
                     LHVQVDAAPELRLQCVANNVRWVDFFILTHGHADHITGMDDLRRFCDLHGGAALTVYT
                     TDEGMARVLSIFPYAIAERPVSRGYAAFKLQELPPQLDLPQGTIQSTLLPHGGLNT"
     gene            698..>1106
                     /locus_tag="VEA63_16340"
     CDS             698..>1106
                     /locus_tag="VEA63_16340"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HYD85639.1"
                     /translation="MSTSSNKTVVVTGAGSGIGRGTALRFLAEGWQVALVGRRSEPLA
                     ETVALGGTSVSARAAIFPCDIGNANAVDAMAAAVLTRFGRVDALVNAAGTNIPRRSWG
                     ELSREDYHAVLATNLNGAVYCVQAFLPGFRAQGG"
BASE COUNT          192 a          394 c          320 g          200 t
ORIGIN      
        1 cgtgttcaaa ccgccgtgcg gcagcagcgt tgactggatc gtcccctgcg gcagatcgag
       61 ttgtggcggc aattcctgca gcttgaacgc cgcataaccg cgcgacaccg gccgctccgc
      121 gatcgcatag ggaaaaatcg acagcacgcg cgccatccct tcgtcggtcg tgtaaaccgt
      181 cagcgccgcc ccgccatgca gatcgcaaaa ccgccgcaag tcatccatgc ccgtgatgtg
      241 atccgcatgc ccatgcgtga ggatgaaaaa atcgacccag cgaacgttgt tcgccacgca
      301 ctgcagccgc agctcgggcg ccgcgtcgac ttgcacgtgc agcccgtcca tcaccacgtg
      361 aatcgacgcc cgcgtgcgct tgttgcgcgg atcgctcgag gtgcaaaccg cgcagtcgca
      421 tgcgatcatc ggcacgcctt gcgaggtgcc cgtcccgaga aacacgactt ccatccggcc
      481 agcgtggcgc gccgctccgc tcggtcaacc tgccgtgcgc gtcgtcgtgc tggccgcgtt
      541 gccgaaaata tttccgctcg atgaacgaaa accactgcgt ttccgtggag cctttctttt
      601 tttcaatcgc ggctaacggc gccgccaccg atttccggga cggtgagttt tgcccggacg
      661 cagccctttc gacaggttac tcctccgtta atacctcatg agcacttcat cgaacaaaac
      721 cgtcgtcgtc accggcgccg gcagcggcat cggccgcggc actgcattgc gcttcctcgc
      781 cgaaggctgg caggtcgccc tcgtgggacg acgctccgaa ccgctcgccg aaactgtcgc
      841 actcggcggc accagcgtct ccgcgcgcgc cgcgattttt ccgtgcgaca ttggcaacgc
      901 gaacgcagtc gacgcgatgg ccgcagccgt gctcacgcgt ttcggccgcg tcgacgcact
      961 cgtcaacgcc gccggcacca acatcccgcg ccgcagttgg ggcgaactct cgcgcgagga
     1021 ttaccacgcc gtgctcgcga ccaatctcaa cggcgccgtc tattgtgtgc aggcattcct
     1081 cccgggtttt cgcgcgcaag gcggcg
//
LOCUS       DASPLT010001602         1738 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_80090, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001602 DASPLT010000000
VERSION     DASPLT010001602.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1738)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1738)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1738
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_80090"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(122..949)
                     /locus_tag="VEA63_16345"
     CDS             complement(122..949)
                     /locus_tag="VEA63_16345"
                     /inference="COORDINATES: protein motif:HMM:NF016817.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase/aspartoacylase
                     family protein"
                     /protein_id="HYD85640.1"
                     /translation="MSLALNTASSCPCSCAHALLEPLRAFVRDDALRAKRVGLTLDSI
                     AGVPGGTQRHEIARVRLTGAYAGHDPIRLGLFAGIHGDEPAGCAALVEFTLALAADPV
                     RFAGYELFIYPVLNPTGLASGTRVNHAGKDLNREFWRDSSEVEVQAIERELRANEFHG
                     IITLHADDTCEGVYGYAHGRTLNEALLGPALAAAKRFLPVDERGVIDGFPARAGLICD
                     CFAGVLSAPPEQRPQPFDVIFETPAHAPFAAQVTATVAALDAIIATYPGFISYAQDL"
     gene            complement(998..>1738)
                     /locus_tag="VEA63_16350"
     CDS             complement(998..>1738)
                     /locus_tag="VEA63_16350"
                     /inference="COORDINATES: protein motif:HMM:NF008022.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0043199 - sulfate binding [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter substrate-binding
                     protein"
                     /protein_id="HYD85641.1"
                     /translation="LDIDILHTQGKLIPADWAKRLPNDSAPSSSTILFIVRKGNPKNI
                     RDWSDLVKPGVGVIIPNPKTSGNGRYSYLAAWGYALLQPGGNEAKAREFVAKLFANVP
                     TLDTGGRGATTSFAQRGIGDVLLTFENEVALTIRELGANELEAVVPSTSIVATNPVAW
                     IDKVVAKKGTGKIAKAYLEYLYSDEAQELAAKHHYRPTNPAILAKYPDRFPPLPLFTV
                     EEIAGSWPTAQKTHFADGGVFDQIYTKH"
BASE COUNT          329 a          580 c          523 g          306 t
ORIGIN      
        1 aaagtatgac cccgcccagg tggaacccga catccccgtc gggttgcttg ataatcgcag
       61 ccgccgaagc ccgacgagga cgtcgagctc cgccgagcgg aagtgtcgtg taacaatgtc
      121 atcagagatc ctgcgcgtaa gaaatgaaac cgggatacgt cgcgatgatc gcgtcgagtg
      181 cggcgaccgt cgccgtcacc tgagctgcga acggcgcgtg cgccggcgtt tcgaaaatca
      241 cgtcgaacgg ctgcggccgc tgctccggcg gcgccgacaa cacgcccgcg aaacaatcgc
      301 agatcaaacc ggcgcgcgcc ggaaagccat cgatcacccc gcgctcgtcg accggcagaa
      361 accgcttcgc cgccgcaagc gccggcccga gcagcgcctc gttcaacgtg cgcccgtgcg
      421 cgtaaccata gacgccttcg cacgtgtcat ccgcatgaag cgtgatgatg ccatgaaatt
      481 cattcgcgcg cagctcacgc tcgattgcct gcacctcgac ctcgctcgag tcgcgccaga
      541 actcgcggtt caagtccttg ccggcgtgat tcacccgcgt gccgctcgcc agtccggtcg
      601 ggttgagcac cggataaatg aacagctcgt aaccagcgaa acgcacggga tccgccgcga
      661 gcgcgagcgt aaattccacc aacgccgcgc agcccgccgg ctcgtcgccg tgaatccccg
      721 cgaaaaggcc gagccgaatc ggatcgtgac cggcgtaagc tcccgtcaaa cgcacgcgcg
      781 cgatttcgtg gcgttgcgtc ccgcccggca cgccggcgat cgagtcgagc gtcaagccca
      841 cgcgcttagc ccgtaaagca tcgtcacgca caaacgcacg cagcggctcg agcagcgcat
      901 gggcgcacga acagggacag gatgaggcgg tattcagggc cagtgacatc gagagagtct
      961 aaagtagggc gggcttcagc ccgccccacg cgtggcgtca gtgcttcgtg taaatctgat
     1021 cgaacacgcc gccgtcggcg aaatgcgttt tctgcgccgt cggccaggag ccggcgatct
     1081 cctcgacggt gaacaacggc agcggcggaa agcggtcggg atacttcgcg aggatcgcgg
     1141 gattcgtcgg ccgatagtga tgcttcgccg cgagttcctg cgcctcgtcg ctgtagagat
     1201 attccaaata cgctttcgcg atctttccgg tgcctttctt cgccacgacc ttgtcgatcc
     1261 acgccactgg atttgtcgct acgatgctgg tcgagggcac caccgcctcg agttcgttcg
     1321 cgccgagttc gcgaatcgtc agcgccactt cgttctcgaa cgtgagcagc acgtcgccga
     1381 tgcctcgctg cgcgaagctc gtcgtcgcac cgcgcccgcc cgtgtcgagc gtcggcacgt
     1441 ttgcgaagag tttcgccacg aattcgcgcg ccttcgcttc gttccctccc ggctggagca
     1501 acgcatagcc ccacgccgcg agataactgt agcggccgtt gccggacgtt ttcggattcg
     1561 gaatgatcac gccgacgccc ggcttcacga ggtcgctcca atcgcgaatg ttcttcgggt
     1621 ttcctttgcg cacgataaac agaatcgtcg aagacgacgg cgcgctgtcg ttcggcaacc
     1681 gcttcgccca gtcggcggga atcagcttcc cttgcgtgtg cagaatatcg atgtcgag
//
LOCUS       DASPLT010001603         3600 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_204201, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001603 DASPLT010000000
VERSION     DASPLT010001603.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 3600)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3600)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3600
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_204201"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..541)
                     /locus_tag="VEA63_16355"
     CDS             complement(<1..541)
                     /locus_tag="VEA63_16355"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HYD85642.1"
                     /translation="MVLPVWNAVGTIGRAVESVRAQTFEDWELIAVNDGSTDGTTEIL
                     RGLAREESRLRVIEQGRAGVAVAANAAMAAARGEFIARMDADDVSHPDRLAEQVAWLD
                     AEWNRSVGVVACLVAFGGDRSVGEGYARHVDWVNALMTPEEIALNRFVEQPVANPSVM
                     FRRELVAQHGGCRDGDFPED"
     gene            complement(879..1358)
                     /locus_tag="VEA63_16360"
     CDS             complement(879..1358)
                     /locus_tag="VEA63_16360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5069 domain-containing protein"
                     /protein_id="HYD85643.1"
                     /translation="MRLLAGRVYESIMNTFIPLISSGTAGPLGVLHLPRFWLKASLEA
                     RGKLDPRYPGCGGGYDMMTLNALGLNKAATLEFIRTAKPTYPQFEAWVKKQPGVKLDA
                     ATIEKHNAAVRGFIHDDETRRGVLKNNGLPDDATAVKDAIRLNDLDSWQEFHEAVLK"
     gene            complement(1534..2133)
                     /gene="msrA"
                     /locus_tag="VEA63_16365"
     CDS             complement(1534..2133)
                     /gene="msrA"
                     /locus_tag="VEA63_16365"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HYD85644.1"
                     /translation="MKRLIALVSLLGFFLPMNDAAEKTKTESIVLGGGCFWCTEAAYD
                     LLPGVKDVVSGYAGGDEPNPTYEDVCAHATGHAEVIKIDYDPAQVSLEAVLDYFWHVH
                     NPTQVGGQGNDIGPQYRSIILYADEAQKAAAEKSRAKAAEEFRDPITTEIVPLKKFWV
                     AEKYHQDYFAKNPNAGYCSYVIAPKIRKLEKQLEGAGKK"
     gene            complement(2245..3252)
                     /locus_tag="VEA63_16370"
     CDS             complement(2245..3252)
                     /locus_tag="VEA63_16370"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HYD85645.1"
                     /translation="MISTADFQHALHCIEAVKAGCDVYVEKPFAETMADARAALKAVR
                     ASDRIVQIGSQRRSGRTYHAAKAFLDSGKFGEIVSVEMTWNVNQPGRWRRPELVAALK
                     EGDVDWKRFLGNRPEAKFDPRVYLEYRLFWPYSSGIPGQWMCHQIDTVHWFTGLAHPR
                     SVSANGGIYLWKDGRTNFDTLTAVFDYGPLDDPTKGFQVTYGSRFTNSAGGTKEIYYS
                     NGGELNLDTRKVTPNGGLEERYAKAMGMKANLLNEMTLDGEVAVTSANTGGDGMTTSH
                     VRNWLECLRSRKQPNAPVEAGYQHAIACIMANAAGRTGQRVTFDEKTQEVMAGGKVFH
                     L"
     gene            complement(3249..>3600)
                     /locus_tag="VEA63_16375"
     CDS             complement(3249..>3600)
                     /locus_tag="VEA63_16375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="twin-arginine translocation signal
                     domain-containing protein"
                     /protein_id="HYD85646.1"
                     /translation="MTSYPLNRREFLRNASVAAAGFALAASGPRLFGQARGANDRIRV
                     GVVGFSDRFRDALLPALKSAMKDINLEIVGVSDLWGRRREEAVAFFAKELGVQVPTYR
                     NNEELYEKAKPDGW"
BASE COUNT          701 a         1420 c          807 g          672 t
ORIGIN      
        1 aatcctccgg aaaatcccca tcccgacacc caccgtgctg cgccaccaac tcccggcgaa
       61 acatcacgct cgggttcgcc accggctgct ccacgaaacg attcaacgcg atctcctccg
      121 gcgtcatcaa cgcgttcacc caatccacgt gccgcgcata cccttcgccc acgctccggt
      181 ctcccccaaa cgccaccaaa cacgccacca cccctacgct ccgattccat tccgcgtcca
      241 gccacgccac ctgctccgcg agccgatccg gatgcgaaac atcgtccgca tccatccgcg
      301 caataaattc acctcgcgct gccgccatcg ccgcattcgc cgccaccgcc acgcccgcgc
      361 gcccctgctc gatcacccgc aaacgcgact cctcccgcgc cagcccccgc aatatctccg
      421 tcgttccgtc cgtcgatcca tcgttcaccg cgatcaattc ccaatcctca aacgtctgcg
      481 cccgcacact ctcaaccgcc cgcccaatcg tccccaccgc attccacacc ggaagaacca
      541 cagagacgcg cggcgataca ttcattcgaa acacgtcgat ctaccgacgc cattccccct
      601 gacgaccgaa atccgcccct ctgacaacag gtagggatgc ctctccgagg cgtccgaagt
      661 tgcacacccg ctccgccaag tcgccgcacg tcataggtgg aaccgacgtc cccgtcgggt
      721 tgtttgagat tcgcagtcgg cttcacactg gcccgccccg ccccgtcccg atcgtgatcg
      781 cgccgcgtcc aaaacccgta gcacggtcaa cctgaccgtg ccgcgccgcg ccgaaagcgc
      841 ggccaaatcg accgcgcttc acagccgggc gctcgcgctc acttcaacac cgcttcgtga
      901 aactcctgcc aactatccag atcgttcaac cggatcgcat ccttcaccgc cgtcgcatcg
      961 tccggcagcc cgttgttctt caaaaccccg cgacgcgtct cgtcgtcgtg aataaacccg
     1021 cgcaccgctg cgttgtgttt ctcgatcgta gccgcatcca gtttcacgcc gggttgcttt
     1081 ttcacccacg cctcgaactg cggatacgtc ggcttcgccg tgcggatgaa ctccagcgtc
     1141 gccgccttgt tcaatccgag cgcgttcaac gtcatcatgt cgtaaccccc accgcagccc
     1201 ggataacgcg gatcgagttt gccgcgcgcc tcgagcgacg ccttgagcca gaaacgcgga
     1261 agatgcagca caccgagcgg acccgccgtg ccggaactga tgagaggaat gaacgtattc
     1321 atgatgcttt cgtaaacgcg acccgcgagc agccgcatcg aggatttgag ggtttaacta
     1381 actgtgcgcc aatcggcgca gccccactga atccaacgat ccataatcct caaggccgca
     1441 acctacctcc ttcccgcccc tcctctcatt cacccatcag tcccatccgt cctataagtc
     1501 ccatcaaccc gtccgcctcc catccgccat cgcctacttc ttccccgccc cttccaactg
     1561 cttctccagc ttccgaatct tcggcgcgat cacatacgag cagtatccag catttggatt
     1621 cttcgcgaag taatcctggt gatacttctc cgccacccag aattttttca gcggcacgat
     1681 ctccgtcgtg atcggatcgc gaaactcctc cgccgccttc gcccgcgact tctccgccgc
     1741 cgccttctgc gcttcatccg catataggat gatcgagcga tactgcggcc cgatgtcgtt
     1801 tccctgccct cccacctgcg tcgggttgtg cacgtgccag aaataatcga gcaccgcctc
     1861 caaagacacc tgcgccggat cgtaatcgat cttgatcacc tccgcgtgcc ccgtcgcatg
     1921 cgcacacacg tcctcatacg tcgggttcgg ctcatcaccg cccgcgtaac cactcaccac
     1981 atccttcacc cccggcagca aatcgtaagc cgcctccgtg caccagaagc agccgccccc
     2041 gagcacgatg gattccgttt tcgttttttc agccgcgtcg ttcatgggaa ggaaaaatcc
     2101 gagcaaactc accaatgcga tcaaccgttt catgcgagta ggagtcacgc tgctcagaac
     2161 tattcccgcc gcaagcgacc acccgtggga ggggcagctg tgggagggct ttacgccccg
     2221 acaagcgccg cccctcccct tctctcacaa atgaaacacc ttcccgcccg ccatcacttc
     2281 ctgcgtcttc tcgtcgaacg tcacgcgctg acccgtccga ccggccgcat tcgccatgat
     2341 gcacgcgatc gcatgctgat aacccgcctc gaccggcgca ttcggctgct tcctcgaacg
     2401 caggcactcc agccagttgc gcacatgcga cgtcgtcatc ccgtcgccgc ccgtgttcgc
     2461 cgacgtcacc gccacttcgc cgtcgagcgt catctcgttc aggaggttcg ccttcatccc
     2521 catcgccttt gcataccgct cctccaaccc gccattcggc gtcaccttcc gcgtatccag
     2581 atttaactca ccgccattcg agtaataaat ttccttcgtc ccgcccgccg aattcgtgaa
     2641 tcgcgaccca tacgtcacct gaaatccttt cgtcggatcg tccaacggac cgtagtcgaa
     2701 caccgccgtc agcgtgtcga aattggtccg cccgtccttc cacaaataaa tcccgccgtt
     2761 cgcgctcacg ctccgcggat gcgccagccc cgtgaaccag tgcaccgtgt cgatctggtg
     2821 acacatccac tgccccggaa ttcccgagga atacggccag aacaaacgat actccagata
     2881 cacgcgcgga tcgaacttcg cctccggccg gttacccaga aaccgcttcc aatccacgtc
     2941 gccttccttc aacgccgcca cgagctccgg ccgacgccag cggcccggct ggttcacgtt
     3001 ccacgtcatc tccaccgaaa cgatctcgcc gaacttcccg gaatccaaaa acgccttcgc
     3061 cgcgtgatac gtccgcccgc tccgccgctg cgagccgatc tgcacgatcc gatcgctcgc
     3121 ccgcaccgcc ttcaacgccg cccgcgcatc cgccatcgtc tccgcaaaag gcttctcgac
     3181 gtaaacgtcg caccccgcct tcaccgcctc gatgcaatgc agcgcgtgct gaaaatccgc
     3241 cgtgctgatc accacccgtc cggcttcgcc ttctcgtaaa gctcctcgtt gttccgatac
     3301 gtcggcactt gcacgcctag ctccttcgcg aaaaacgcca ccgcttcctc gcgccgccgt
     3361 ccccaaagat cggatacacc cacgatctcg aggttgatgt ccttcatcgc gctcttcagc
     3421 gccggcagca gcgcatcccg gaagcggtcc gaaaaaccca ccacgccgac ccgaatccgg
     3481 tcattggcgc cgcgcgcctg tccaaacaaa cgcggtccgg acgccgcgag cgcaaagccc
     3541 gccgccgcca ccgacgcatt ccgcaaaaac tcacgacgat tcaaggggta ggaagtcatg
//
LOCUS       DASPLT010001604         1048 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_121117, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001604 DASPLT010000000
VERSION     DASPLT010001604.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1048)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1048)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1048
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_121117"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..420)
                     /locus_tag="VEA63_16380"
     CDS             complement(<1..420)
                     /locus_tag="VEA63_16380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009509684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreD"
                     /protein_id="HYD85647.1"
                     /translation="MAEFCGHLRLRGAVREHGRTVLAEQSFRAPFHLSKPYWDADAGV
                     LIVQVVNPTAGILAGDRLESDIAVDAGAAVCVTTPSASRVFKMKDGVAECRQRFCVAA
                     GGWLEVMPEPLVPHRGCVYRQVTTVEVEAGGALFFVDQ"
     gene            complement(520..>1048)
                     /gene="ureG"
                     /locus_tag="VEA63_16385"
     CDS             complement(520..>1048)
                     /gene="ureG"
                     /locus_tag="VEA63_16385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012377014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="urease accessory protein UreG"
                     /protein_id="HYD85648.1"
                     /translation="MAVVTNDIYCSEDAQFLIKHSALPAERISGVETGGCPHTAIRDD
                     TTMNEQACRALEAKFSDLQVVLVESGGDNLTATFSPELVDAFIYVIDVAEGDKIPRKG
                     GPAIRFSDLLIVNKIDLAPLVGADLGVMERDAKVQRGARPTLFCDLKREHNLDAVIAW
                     IEREVLFGRAARATG"
BASE COUNT          216 a          414 c          228 g          190 t
ORIGIN      
        1 ctgatccaca aaaaacagcg cgccgcccgc ctccacttcc accgtcgtca cctgccgata
       61 aacgcagccc cgatgcggca ccaacggctc cggcatcact tccaaccagc cccccgccgc
      121 cacgcaaaac cgctgccggc actccgcgac gccatccttc atcttgaaaa cccgactcgc
      181 actcggcgtc gtcacacaca ccgccgcgcc tgcgtcgacc gcgatatccg actccagccg
      241 atcgcctgcc aaaattcccg ccgtcggatt caccacctgc acgatcaaca cgcccgcatc
      301 cgcatcccaa tacggcttgc tcagatgaaa cggcgcgcga aacgattgct ccgcgagcac
      361 cgtccgccca tgttcccgca ccgctccccg caaccgcaaa tgtccacaaa actcagccat
      421 gggagtggct catcatcgtt ctctttctcg taatcgttct ctttctcgtt ctcctcccct
      481 cccgtccatc cgacgagaga acgaagaacg agaacgattt taacccgtcg ctctcgccgc
      541 ccttccaaac aacacctcac gctcgatcca agcgatcacc gcgtcgaggt tatgctcgcg
      601 cttcaaatcg cagaacaacg tcggccgcgc cccacgctgc actttcgcat cccgctccat
      661 caccccgagg tccgcgccca ccaacggcgc caaatcgatt ttgttcacga tcagcaaatc
      721 gctgaaccga atcgccggcc cgcctttccg gggaatttta tccccctccg ccacgtcgat
      781 cacgtagatg aatgcatcga ccagctccgg cgaaaacgtc gccgtcaaat tgtcgccgcc
      841 gctctccacg agcaccacct gcaaatcgga aaatttcgcc tcgagcgccc gacacgcctg
      901 ctcgttcatc gtcgtgtcgt cgcgaatcgc cgtgtgcgga cacccgcccg tctccactcc
      961 gctgatacgc tcggcgggca gcgcgctatg cttgatcagg aactgagcgt cttcggagca
     1021 gtagatgtca ttcgtgacga ccgccatc
//
LOCUS       DASPLT010001605         1066 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_121133, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001605 DASPLT010000000
VERSION     DASPLT010001605.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1066)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1066)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1066
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_121133"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(252..1052)
                     /locus_tag="VEA63_16390"
     CDS             complement(252..1052)
                     /locus_tag="VEA63_16390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012377408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HYD85649.1"
                     /translation="MSAADVQAEALPAIAVAELACGYDGRTILKDVSFNVRRGEIFFV
                     IGGSGCGKSTLLRNLIGIHTPMAGSVKFFGESFTDARTKRQRELLKTFGVLYQGAALW
                     TSLTLRQNVELPLEEYTTLSRREREEIATLKLAQVGLTGSEDFYPAEISGGMKKRAGL
                     ARALALDPSILFLDEPSAGLDPITSRKLDDLVLELRDLFGTTMVVVSHELASIFSIAD
                     RVIMLDREQQGIIAEGAPRTLAAESRDVRVLEFLRRGDLPPGTIGEHK"
BASE COUNT          228 a          409 c          251 g          178 t
ORIGIN      
        1 gcccgtgaat cgactcgtcg aaaaacacca cgaaacgctg cggcttcgaa aagaaactca
       61 ccccgccaaa cgacagcagc gcaacgatgc cgagcacgaa cgctccaatc acaaatgcac
      121 cgacgatggc tgggcctgat ttggatttca ccgggttgaa gagagaatcg attccgcacg
      181 cgcgcaacgc tgcgccgcaa tcctgctgga ttgcttcgtc gtcgcgcttc tcgcaatgac
      241 aaacatttcc gtcacttgtg ctctccaatc gtgcccggcg gcagatcgcc gcggcgcaga
      301 aattccagca cccgcacgtc gcgactttcc gccgccagcg tccgcggcgc gccctccgca
      361 atgatcccct gctgctcccg atcgagcatg atcacccggt ccgcgatgct gaaaatcgac
      421 gccagctcgt gcgacaccac caccatcgtc gtcccaaaca aatcccgcag ctcgagcacc
      481 aggtcatcga gcttgcgcga cgtgattgga tccagccccg ccgacggctc gtcgagaaac
      541 aaaatcgacg gatccaacgc cagcgctcgc gccagtcccg cgcgtttctt catgccgccg
      601 ctgatctccg ccggataaaa atcctccgaa cccgtcagcc cgacctgcgc cagcttcagc
      661 gtcgcgattt cctcccgctc gcgccgcgat aacgtcgtgt attcctccag cggcaactcg
      721 acattctgcc gcagcgtcaa cgacgtccac agtgccgcgc cctgatacaa cacgccgaac
      781 gtcttcaaca actcccgctg ccgcttcgtc cgcgcatcgg tgaacgactc gccgaaaaat
      841 ttcaccgagc ccgccatcgg cgtgtgaatg ccaatcaaat tccgcaacag cgtgctcttc
      901 ccgcaacccg acccgccgat cacgaaaaaa atctccccgc gacgcacgtt gaacgacacg
      961 tctttcaaaa tcgtccgccc atcgtaaccg cacgccagct ccgccaccgc gatcgccggc
     1021 aacgcctccg cctgcacgtc cgccgcgctc atgcgtccgc ctccca
//
LOCUS       DASPLT010001606         1062 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_183983, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001606 DASPLT010000000
VERSION     DASPLT010001606.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1062)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1062)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1062
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_183983"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            77..>1062
                     /locus_tag="VEA63_16395"
     CDS             77..>1062
                     /locus_tag="VEA63_16395"
                     /inference="COORDINATES: protein motif:HMM:NF012935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HYD85650.1"
                     /translation="MPVITASKTQPNYDVIVVGSGAGGGQAAYTLTMAGVKVLMLETG
                     RYYDPVKETPMFNIAAQAPLRASQTPDKPLGFFDATIDGGWSVPGEPYTNASTERGRD
                     FTWWRARMLGGRTNHWGRISLRNGPYDFKPYSRDGLGRDWPISYEDLAPYYDKVEMLI
                     GVYGSNEGLENTPDSSPGVLLPPPKPRIGELLAQNRAKKLGIPVIPIHRAVLSTRQDA
                     DRIPPLLHPGNLKAQRLLAESMRARAACFWATECNRGCSIKANYQSPTVHLPPALASG
                     NLDIVTDAMVRAVLTDENGKATGVSYIDRPTGQERTVKARVVVLAASSAESVR"
BASE COUNT          202 a          414 c          263 g          183 t
ORIGIN      
        1 ctcactccga cctcgctgct tggatttcgc atccttcttt ggcgtttggc cctttggcct
       61 ttgagatttc ttcctgatgc ccgtcatcac cgcctccaaa acccaaccta actacgatgt
      121 gattgtcgtc ggctccggcg ccggcggcgg ccaggccgct tacacgctca caatggccgg
      181 cgtgaaagtc ctgatgctcg agaccggtcg ctactacgac ccggtcaaag aaacaccgat
      241 gttcaacatc gccgcgcaag ctcctctccg cgcttcgcag acgcccgata aaccgctcgg
      301 ctttttcgac gccacgatcg acggcggctg gagcgttccc ggcgaaccct acaccaacgc
      361 ctcaaccgaa cgcggccgcg acttcacctg gtggcgcgca cgcatgctcg gcggacgcac
      421 gaatcactgg ggacgcatct cccttcgcaa cggaccttac gatttcaaac cctacagccg
      481 cgacggcctc ggccgcgact ggccgatctc ttacgaagac ctcgcgccct actacgacaa
      541 agtcgagatg ctcatcggcg tctacggctc caacgaaggc ctcgaaaaca cgcctgattc
      601 ctcgcccggc gttttgcttc caccgccgaa accccgcatc ggcgaactgc tcgctcagaa
      661 ccgcgcgaaa aaactcggca tccccgtcat ccccattcac cgcgccgtcc tctccacgcg
      721 ccaggacgcc gatcgcattc cgccgctact ccaccccggc aacctcaaag cccagcgcct
      781 cctggccgaa tcgatgcgcg cgcgcgccgc gtgcttctgg gcgaccgagt gcaatcgcgg
      841 ttgctcgatc aaagccaact accaatcgcc caccgtccac cttccgcccg cgctcgcgag
      901 cggcaacctc gacatcgtca ccgacgcgat ggtccgcgcc gtcctcaccg acgaaaacgg
      961 caaagccacc ggcgtttcct acatcgacag accgacgggg caggaacgca ccgtcaaagc
     1021 ccgcgtcgtc gtcctcgccg ccagctccgc cgaatccgtt cg
//
LOCUS       DASPLT010001607         1266 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_183988, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001607 DASPLT010000000
VERSION     DASPLT010001607.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1266)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1266)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1266
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_183988"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..731)
                     /locus_tag="VEA63_16400"
     CDS             complement(<1..731)
                     /locus_tag="VEA63_16400"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HYD85651.1"
                     /translation="MKYALRSLARSPGFTAVAALTLALGIGANTTFFSVLYGVVLRGL
                     PFPNSSELVELRHHGGPSNNDNGRISLAELHDFRDRQRSLAGLGAYDIGRTTLNLPDG
                     AERIIQTDVTANLFSILGIPPALGRSFTTDDERAGNNNVVIVSHAFWQNHLAGSPDAI
                     GLTLRLNGREHTVVGVMPATFFFSFGETGTALWKPLDLSAGASGARDDRSLSTLARLA
                     PNVSLDAARGDFERVARQLQTDLPAA"
     gene            complement(973..>1266)
                     /locus_tag="VEA63_16405"
     CDS             complement(973..>1266)
                     /locus_tag="VEA63_16405"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HYD85652.1"
                     /translation="RTREIGLRMALGAQPRDVARAILRETLVVTALGIGVGLPVTFAL
                     NRLIKAQLYNVQPNDTLTLVVTVASLLAVALTAAWLPAHRAAKVNPMTALRAE"
BASE COUNT          228 a          304 c          518 g          216 t
ORIGIN      
        1 gcggcgggga gatcggtttg gagttggcgg gcgacgcgtt cgaagtcgcc gcgcgcggcg
       61 tcgagcgaaa cgttgggcgc gagccgcgcg agggtggaca gggagcggtc gtcgcgagcg
      121 ccggacgcgc cggcggagag atcgagcggt ttccagaggg cggtgccggt ttcgccgaag
      181 gagaagaaaa acgtggcggg catgacgccg acgacggtgt gctcgcggcc gttcagacga
      241 agcgtgaggc cgatggcgtc gggcgagccg gcgagatggt tttgccagaa ggcgtggctg
      301 acgatgacga cgttgttgtt tccggcgcgc tcgtcgtcgg tggtgaaaga acggccgagc
      361 gcgggaggga taccgagaat ggaaaagagg ttcgcggtga catcggtctg gatgatgcgt
      421 tcggcgccat cgggcagatt gagcgtggtg cggccgatgt cgtaggcgcc gaggccggcg
      481 aggctgcgct ggcgatcgcg aaaatcgtgg agttcggcga gggagatccg gccgttgtca
      541 ttgttcgatg ggccgccgtg gtgacggagt tcgacgagtt cgctggagtt gggaaacggc
      601 aggccgcgca ggacaacgcc gtagagcacg ctgaaaaacg tggtgttggc tccgatccca
      661 agcgcgagcg tgagcgcggc gacggcggta aagccgggcg atcgggcgag ggagcggagg
      721 gcgtatttca tggcgaggga cgggcgtcgg gacggaggtt ttaaccgcta atctgcgcta
      781 atttttccga ggagggatgg cggatgaggg gttgcgtgct tcgcctcatc ggcggagcgc
      841 gggcgatttt tgggagaagg agcgatgatg gaggattggt cgttctcgtt ctctttatcg
      901 ttctcgttct ctcggcttcg gtgggatgga ttcgggtcga gaacgagaaa gagaacgaag
      961 aacgagaacg atttactcgg cgcggagggc ggtcatgggg tttactttgg cggcgcggtg
     1021 cgcgggcagc cacgcggcgg tgagggccac ggcgagcaga gaggccacgg tgacgacgag
     1081 cgtgagtgta tcgttgggct gaacgttata gagttgggcc ttgatcagcc ggttgagggc
     1141 gaaggtaaca ggcagaccca ccccgatgcc gagagccgtt acgacgagcg tctcacgcag
     1201 gatagcgcgc gccacgtcgc gcggctgagc gccaagcgcc atgcgcaagc cgatctcgcg
     1261 cgtgcg
//
LOCUS       DASPLT010001608         1162 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_19789, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001608 DASPLT010000000
VERSION     DASPLT010001608.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1162)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1162)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1162
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_19789"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1162)
                     /locus_tag="VEA63_16410"
     CDS             complement(<1..>1162)
                     /locus_tag="VEA63_16410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD85653.1"
                     /translation="WVELQTFAGSNGVTSHTVSLAGYESADTRIAFRIDTNFSNNEFR
                     YVDNVTISAESDANYAGAAYTEQGAPVSIAAADAIVVDGDSANLSRMVLAVDNYVQGD
                     RITFDGAAISLVNGASGTTGSGFAYAVAISGDKASLTLTRTTTPANFQAALRAATFDS
                     SSDDPTAGGASTTRSISVVAYDTTGLQSNTAVSTVSVTAVADAPTVDAPLIPVSDDFS
                     SDSYAGGTGWAGPWVESEGGSAAAGDARVTGERLRLETNTWAAREADLSAYAAGYTAR
                     LNVDVSFAATTYEAADIAYVEISNDGGATFTVLEQLGNLGSNPAALSRSYDISGQVSD
                     QTVVRFRVEGFASGVEFLYVDNVSFSVEPAPYTGTFTEDAGSVPVVDPDIDVA"
BASE COUNT          202 a          382 c          372 g          206 t
ORIGIN      
        1 ccgcgacatc gatgtccggg tccacgaccg gcactgagcc ggcatcttcc gtaaaggtgc
       61 cggtataggg cgccggctcg acgctgaagg agacgttgtc cacgtataga aactccacgc
      121 cggaggcgaa accttcgaca cggaagcgca caaccgtctg gtcggacacc tgcccggaga
      181 tgtcgtagct ccgcgaaagc gcggcagggt tggagccgag gttgccgagc tgctcgagaa
      241 ccgtgaaggt ggcaccgccg tcgttagaga tttcaacgta ggcgatgtcc gccgcctcgt
      301 aggtcgtagc cgcgaagctg acatccacgt tcagtcgcgc ggtgtagccg gcggcgtacg
      361 ccgacagatc cgcctcgcgc gccgcccagg tattcgtttc tagccgcaag cgctcgcccg
      421 tcaccctcgc gtcgccggcc gctgcgcttc cgccctccga ctccacccaa ggaccggccc
      481 agccggtgcc gcccgcatac gaatccgacg agaagtcgtc gctgacaggg atcagcggcg
      541 catcgacggt tggggcgtcc gcaacagcgg tgacgctcac cgtcgatacc gccgtattgc
      601 tctgcaggcc tgtggtgtcg tacgcgacga ctgaaatgct gcgcgtcgta ctagcgccgc
      661 ctgccgtcgg atcgtcggag ctcgagtcga acgtcgcggc ccgcagcgcg gcctggaagt
      721 tcgccggggt agtcgtgcgc gtgagcgtca ggctcgcctt atcgccgctg atcgcgaccg
      781 cataagcaaa gccgcttccc gtggttccgc tcgccccgtt cacgagcgaa atggcggcgc
      841 cgtcgaaggt gatccgatcg ccctggacgt aattatccac ggcgagcacc atcctggaga
      901 gattggcgct gtccccatcc acgacgatcg catcggccgc ggcgatggaa accggagcgc
      961 cttgctccgt gtatgccgca ccggcataat tcgcgtccga ttccgccgag atggtgacgt
     1021 tgtcgacgta gcggaattcg ttgttgctga aattggtgtc gatgcggaac gcgatcctcg
     1081 tatccgcgga ctcgtagccg gcaaggctga ccgtatgcga cgtgacgccg ttgctgccgg
     1141 caaaggtttg cagttccacc ca
//
LOCUS       DASPLT010001609         1908 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_243800, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001609 DASPLT010000000
VERSION     DASPLT010001609.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1908)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1908)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1908
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_243800"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            503..1555
                     /locus_tag="VEA63_16415"
     CDS             503..1555
                     /locus_tag="VEA63_16415"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HYD85654.1"
                     /translation="MSSSSAPLGIGILGLGMVADFHAQAIAHVKGARLVAVATRNGDK
                     ARAFAQKHGAVWSGTSIEALVAQPGVDIVCITTPASAHLDPALVAIAAGKHLVIEKPL
                     EITVERTDAILRAADAAGVKIAAIFQGRFGEGAQTVKAALEAGRFGRLVLASAYVKWH
                     RTAQYYTGTRGTLTVDGGGALMAQAIHAIDLLQWFAGMPSELFCWATRRVYGHIEGED
                     TASAALKFADGTLGTIEASTALWPGWQRRIEICGEHGSVALEDDHITRWQFREEGAAD
                     ETIRAAKDAAALGSGASAPNAISFVGHQRQIQDLVDAIREGRRPAVDGHEGRKAVALV
                     RAMYDSAARGAAVKIG"
BASE COUNT          343 a          492 c          739 g          334 t
ORIGIN      
        1 aaaccgcgaa gttaacgacg gattggatca cgatttttag gacgaggaga ccgagggcgg
       61 tgcggaaaaa taaaatccag cgactgcggt gagcgacgcg ggcgatcgcg gccggagcct
      121 ggcgcggggg tttgagcgcg agcgacatgg aaggtcggct ggacgaggaa gagagcgcgt
      181 cggcagaacg atctggggcg ccacaggagc tgggcgcccg gcagtttcac tgcaattgga
      241 aagcagttgg tttgccgcga atcaggggaa ggctgatgtt gcggcggcgg ggcagacgcg
      301 tgcgaacggt aaagaaccga cgtgaagcaa cggcgagggc gcggtgaaca caggcgtcct
      361 atcgagcgag ctcggcggac gcgttgcggc agcacggatc gcgtgagaga accgcttgcc
      421 gcatcttttt agttacggcc gcgtcacttg ccttgcctgc ggcagtttgg gtctacgatg
      481 ctcgcgcggc ggatttttca cgatgagttc ctcttctgcg cccctcggca tcggtattct
      541 aggtctcggc atggtggccg attttcatgc gcaggcgatt gcgcacgtga agggcgcgcg
      601 attggtcgcg gtggcgacgc gcaatggcga caaggcgcgg gcgtttgcgc aaaagcacgg
      661 cgcggtgtgg tcgggcacga gcatcgaggc gctcgttgcg cagccgggcg tggacatcgt
      721 gtgcattacg acgccggcga gcgcgcacct cgatccggcg ctggtcgcga ttgcggcggg
      781 gaaacatttg gtgatcgaga agccgctgga gatcacggtg gagcgcacgg acgcgatatt
      841 gcgcgcggcg gacgcggcgg gcgtgaagat cgcggcgata tttcaggggc gtttcggcga
      901 aggcgcgcag acagtgaaag cggcgctcga ggcggggcgt tttggccggc tggtgttggc
      961 gagcgcgtat gtgaaatggc atcgcacggc gcagtattac acggggacgc ggggaacgct
     1021 cacggtggac ggcggcggag cattgatggc gcaggcgatt cacgcgatcg atctgctgca
     1081 atggttcgca ggaatgccgt cggagttgtt ttgctgggct acgcggcggg tgtatgggca
     1141 tatcgaaggt gaggatacgg cgagcgcggc gctgaaattt gccgacggca cgctcgggac
     1201 gatcgaggcg agcacggcgt tgtggccggg atggcagcga cggatcgaaa tctgcggcga
     1261 gcacggatcg gtggcgttgg aggacgatca catcacgcgg tggcaatttc gcgaggaggg
     1321 cgccgcggat gaaacgattc gagccgcgaa ggacgcggcg gcgctcggtt cgggcgcgag
     1381 cgcgccgaac gcgatcagtt tcgtcggcca tcagcggcag attcaggatc tcgtcgacgc
     1441 gatccgcgaa ggtcggcggc cggcggtgga cggacacgaa gggcgtaagg ccgtggcgct
     1501 ggtgcgcgcg atgtatgatt cggcggcgcg aggcgcggcg gtgaagatcg ggtgagcgtc
     1561 gacgctacga cgatgcgtgc gcggcttgac cgagacgtag agatctacag gcggcatcag
     1621 ttcggcggag ttttcggttc acggccgtcg cgggagcagg ggtttcaagg cgtgttacac
     1681 agagaacaca gagcgtcgac atggagaagc cggaggccac gaggtggtcg gaggggggcg
     1741 tcgcgtccga cgtgctgagc gtgtttctat tttcatgacc ccaatcctga ccccgcccgt
     1801 tcaatccgcc tggtggcgca cgctgacgcg cgagcactgg ctcgttttcg tcggtgcatc
     1861 gctggcctgg ctgttcgatt gcctggattc gcagctgttc aatctcgc
//
LOCUS       DASPLT010001610         1298 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_284920, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001610 DASPLT010000000
VERSION     DASPLT010001610.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1298)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1298)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1298
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_284920"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..369
                     /locus_tag="VEA63_16420"
     CDS             <1..369
                     /locus_tag="VEA63_16420"
                     /inference="COORDINATES: protein motif:HMM:NF018028.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HYD85655.1"
                     /translation="AGFWIRIAASLLDILIVAIAGNMIPDRWQPNLLLIFAAYCCVLW
                     ATKGTTVGGIICGLKVVRLDERPIDWPTALVRALGGFLSLVAAGIGFIWVAFDDQKQS
                     WHDKIAGTTIVHVPRGVSLV"
     gene            442..930
                     /gene="purE"
                     /locus_tag="VEA63_16425"
     CDS             442..930
                     /gene="purE"
                     /locus_tag="VEA63_16425"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HYD85656.1"
                     /translation="MSKPLVGIIMGSTSDWETMRHAAAQLTALGVPFEKRVVSAHRTP
                     KLMMTYAESAERRGLKVIIAGAGGAAHLPGMTASMTTLPVLGVPVESKTLKGLDSLLS
                     IVQMPGGVPVATFAIGKAGAINAALFAASLLAQSDANTAKAWRKFRADQTATVLKAKL
                     PD"
     gene            1004..>1298
                     /locus_tag="VEA63_16430"
     CDS             1004..>1298
                     /locus_tag="VEA63_16430"
                     /inference="COORDINATES: protein motif:HMM:NF014841.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HYD85657.1"
                     /translation="MILPGKTIGILGGGQLGRMLGQAAQTLGYRVHVFEPHKNCPAGA
                     VANREVNASYEDLAALKEFARGVDVITYEFENIPSEPLAAIAPLVPLHPRSDVL"
BASE COUNT          212 a          330 c          493 g          263 t
ORIGIN      
        1 gcgggttttt ggattcgaat cgcggcgtcg ttgctcgaca tcctgatcgt ggcgattgcg
       61 ggcaatatga ttcccgatcg ctggcagccg aatctgttgc tgattttcgc ggcgtattgc
      121 tgcgtgttgt gggcgacgaa ggggacgacg gtcggcggga ttatctgcgg attgaaagtc
      181 gtgcgcttgg acgagcggcc gatcgactgg ccgacggctt tggtgcgcgc gctcggtggt
      241 ttcctttcgc tggtggcggc gggcatcgga ttcatctggg tggcgttcga cgaccagaag
      301 cagtcgtggc acgacaagat cgcgggcacc acaatcgtgc atgtgccgag aggcgtgtcg
      361 ctggtgtaga gcggagagcg ggtggcggtc gcgaaaatcc gcattgctgt aatccgtaaa
      421 cgcgggttac agctcgggct catgtcgaag ccacttgtcg ggatcatcat gggtagcacg
      481 tccgattggg agacgatgcg gcacgcggcg gcgcaattga cggcgttggg cgtgccgttc
      541 gagaaacgcg tcgtgagcgc gcatcgcacg ccgaagttga tgatgactta tgcggagagc
      601 gcggagcgtc gggggttgaa agtcatcatc gcgggcgcgg gtggcgcggc gcatttgccg
      661 gggatgacgg cgtcgatgac gacgctgccg gtgttgggcg tgccggtgga atcgaagacg
      721 ttgaagggtc tcgattcgct gctttcgatc gtgcaaatgc cggggggcgt gccggtggcg
      781 acgttcgcga tcggaaaagc gggcgcgatc aacgcggcgt tgtttgcggc gtcgttgctc
      841 gcgcagagcg atgcgaacac cgccaaggcg tggcggaaat ttcgcgcaga ccagacggcg
      901 acggtgttga aggcgaaact gccggactga gctggcgggt ggggcgacgg aagagttttg
      961 acgcggggcg gggcgtctcc gttgtctttc gtttcgcgtg caaatgattc ttcccggaaa
     1021 aacgatcggt atcctcggcg gcggccagct tgggcgcatg ttggggcagg cggcgcagac
     1081 gctcggttat cgcgtgcacg tgttcgagcc gcataagaac tgtccggcgg gcgcggtcgc
     1141 gaaccgcgag gtgaacgcga gctacgagga tctggcggcg ttgaaggagt tcgcgcgcgg
     1201 tgtcgatgtg atcacttacg agttcgagaa cattccctcg gagccgctcg cggcgatcgc
     1261 gccgctggtg ccgctgcacc cgcgctcgga tgttctgc
//
LOCUS       DASPLT010001611         1094 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_265926, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001611 DASPLT010000000
VERSION     DASPLT010001611.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1094)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1094)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1094
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_265926"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1094
                     /locus_tag="VEA63_16435"
     CDS             <1..>1094
                     /locus_tag="VEA63_16435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HYD85658.1"
                     /translation="YEPLKGHRFNANKLLIDPYAKAIAGEVEWADENFGYTIGDPQED
                     LSIDTRDNAALMPKAVVVDPAFAWGDDARPNRPLHETVIYEVHVKGFSKLWDALPEAV
                     RGTYSGLGSPQAIEYFKHLGVTAIELLPVHQHVNSKHLLDKGLTDYWGYNTLGFFAPE
                     PTYRSKAEFASEVDEFRTMVRSLHAAGLEVILDVVYNHTAEGNHLGPTLSFRGIDNAS
                     YYRLVGDSPRHYMDYTGTGNTLNVPHPRVLQLVMDSLRYWVSEMHVDGFRFDLAPALA
                     RELHEVSRLSSFFDVIHQDPVISQVKLIAEPWDVGEGGYQVGNFPVLWAEWNGRYRDT
                     VRRYWKGESGHMGDFAYRLCGSSDLYEGSG"
BASE COUNT          212 a          324 c          353 g          205 t
ORIGIN      
        1 cgtatgagcc gctaaaaggg caccggttca acgcgaacaa gctcttgatc gatccgtatg
       61 cgaaggcgat cgcgggtgag gtggaatggg cggacgaaaa tttcggctac acgatcggcg
      121 acccgcagga agacctgtcg atcgacacgc gcgacaatgc cgcgctgatg ccgaaagcag
      181 tggttgtcga cccggcgttc gcctggggtg acgacgcgcg gccgaaccgg ccgctgcacg
      241 agacggtgat ttacgaagtg catgtgaaag gtttttccaa gctttgggat gcactccccg
      301 aagcggtgcg cggcacctac tcaggcctcg gcagtccgca ggcgatcgag tatttcaagc
      361 atctcggcgt cactgcgatc gagctgctgc cggttcacca gcacgtgaat tcgaagcacc
      421 tgctcgacaa aggactcacc gattactggg gctacaacac gctcggtttt ttcgcgccgg
      481 agccgacgta tcgcagcaag gcggagttcg cgtcggaggt ggatgagttt cgcacgatgg
      541 tgcgcagcct gcacgccgcc ggactcgaag tgattctcga cgtcgtttac aaccacaccg
      601 ccgagggcaa ccacctcggg ccgacgctga gtttccgcgg catcgacaac gcgagttact
      661 accgcttggt cggcgacagc ccgcggcact acatggacta caccggcacg ggcaacacgc
      721 tcaacgtgcc gcatccgcgc gtgctgcagc tcgtgatgga cagcttgcgc tactgggtat
      781 cggagatgca cgtcgacgga ttccgcttcg atctcgcgcc ggcgctcgcg cgcgagttgc
      841 acgaggtcag ccgactgtcg tcgttcttcg acgtcattca tcaagatccc gtcatctcgc
      901 aggtgaaact catcgcggag ccgtgggacg tcggcgaggg ggggtatcaa gtgggcaatt
      961 ttccggtgtt gtgggcggag tggaatggcc gctatcgcga caccgtgcgc cgctactgga
     1021 aaggcgagtc ggggcacatg ggcgacttcg cttatcgtct ttgcggcagc tccgatctct
     1081 acgaaggcag cggc
//
LOCUS       DASPLT010001612         1479 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_19877, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001612 DASPLT010000000
VERSION     DASPLT010001612.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1479)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1479)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1479
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_19877"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..225
                     /locus_tag="VEA63_16440"
     CDS             <1..225
                     /locus_tag="VEA63_16440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007360487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HYD85659.1"
                     /translation="GASPRASLALYQASRALAWLRGQDYVSPAIVQELAPDVMRHRIG
                     LTYEAEAEEITADKIVAQVTAQTPVPAAKV"
     gene            242..1162
                     /locus_tag="VEA63_16445"
     CDS             242..1162
                     /locus_tag="VEA63_16445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HYD85660.1"
                     /translation="MLFGKRAAESDSQPSGPSALSLLRQLEWRVRHAVENVLSGEYRS
                     TFRGRGMEFDQVVRYEFGDDVRDIDWNVTARLGEPYRKKFVEEREITLLVVFEDSPSL
                     EFGSGAQSKREALLELAGLVMLLGAVNRDRVGFVYASPAGNLFRQPVRGRGAILHAAA
                     TLLAQPPPLISDLPSPNSDIPWRFLARAAPKHSVLLWLGDFPPRVQPDGWPVIQRRYQ
                     TMGFRVDDPWERELPAGETFSAYDPLSGRLVTLEGSSAERAAHAAWRDERDAAWRALF
                     PAPLSRLVVSTEENRLDALVRFFHARMNAR"
BASE COUNT          239 a          560 c          404 g          276 t
ORIGIN      
        1 ggcgcctctc cgcgcgcatc gctcgcgctg tatcaagcca gtcgcgcgct cgcatggctt
       61 cgcggccagg attacgtttc tccggccatc gtgcaggagc tcgcacccga tgtcatgcgc
      121 catcgcatcg gtctcaccta cgaagccgaa gccgaagaaa tcacggccga caagatcgtc
      181 gctcaggtca ctgcgcagac gcccgtgccc gcggccaagg tctgagtctc cgctcagggc
      241 cttgctgttt ggaaaacgcg cggctgagtc agactctcag ccttccggcc cttcagctct
      301 ctcgctgctg cgtcagctcg agtggcgcgt gcgccacgcc gtggaaaacg tgctgagcgg
      361 cgagtatcgc tccacgtttc gcggccgcgg catggagttc gatcaagtcg tccgctacga
      421 attcggcgac gatgtgcgcg acatcgattg gaacgtcacc gcgcgcctcg gcgaacccta
      481 ccgcaaaaag ttcgtcgagg aacgcgagat cacgctcctc gtcgtcttcg aagattcccc
      541 gtctctcgaa ttcggctccg gcgcgcaatc caaacgcgaa gccctccttg agctcgccgg
      601 cctcgtcatg ctcctcggcg ccgtcaaccg cgaccgcgtc ggcttcgtct acgcctcgcc
      661 cgccggcaat ttgtttcgtc aacccgtccg cggccgcggc gcgatccttc acgccgccgc
      721 cacgctgctg gctcagcctc cgcccctgat ctccgatctc ccatcgccga actccgacat
      781 cccgtggcgc tttctcgcgc gcgccgcgcc gaagcacagc gtgcttctct ggctcggcga
      841 cttccctccg cgcgtgcaac ccgacggctg gcccgtcatc cagcgccgtt atcaaacgat
      901 gggctttcgc gtcgacgatc cgtgggaacg cgaactcccc gcgggcgaaa ccttctccgc
      961 ctacgacccg ctctccggac ggctcgtcac gcttgaaggc agctccgccg aacgcgccgc
     1021 gcacgccgca tggcgcgatg aacgcgacgc cgcctggcgc gcgctctttc ccgcgccgct
     1081 cagccgcctc gtcgtcagca cggaagaaaa ccgcctcgat gcgctcgtcc gctttttcca
     1141 cgcccgcatg aacgcgcgct gacgcgcgcg aagctccaag cgatcagaag ctccaagcac
     1201 caacctccaa gctccagtga aacatcaaac cccaaacacc cgcttccatc cctaacgtgt
     1261 tcgccgaccc caccaactcc gcgccttccc cgagccaaat tgaagtttgg ctccgcttgg
     1321 accttcattg gagcttggag gttggagctt ggagctttgc ccgcgcggct tggagcttcc
     1381 cgccgcctcg atgaacgact tcacgttcca cagcccttat tggttcagcg cactgatcgc
     1441 tgttgccgtc gtcatgattc tgcggcgccg ccgcagcgt
//
LOCUS       DASPLT010001613         1952 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_265935, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001613 DASPLT010000000
VERSION     DASPLT010001613.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1952)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1952)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1952
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_265935"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            192..>1952
                     /locus_tag="VEA63_16450"
     CDS             192..>1952
                     /locus_tag="VEA63_16450"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HYD85661.1"
                     /translation="MNTTRREFVRRSAALAAYTAAFGTLRGEASRRTDDTGPDREPQW
                     ILYNGKIATCDETRPFAEAVAIRDGRFLAVGASDFILQLGGPSTRVVDLAGRTAIPGL
                     NDSHLHVIRGGLNYNLELRWDGVPSLADGLRMLREQAARTPAPQWVRVVGGWSEFQFA
                     EKRLPTLDEINEAAPDTPVFILHLYCQAMLNRAALRAVGYDKNTPNPPGGEIQRDKSG
                     NPTGLLLARPNAAILYATLAKGPKLPLDYQYNSTRQFMRELNRLGVTSVIDAGGGFQN
                     YPEDYQVIDELHRRGELTLRIAYNLFTQKPQQEKDDFARWIRMAKPGQGDDVLRLNGA
                     GEMLVFSAADFEDFLEPRPDLATRLESDLEGVISLLAANRWPFRLHATYDESITRFLN
                     VIEKVNRAHPLDGLHWFLDHAETIGERNIERVRALGGGVAVQHRMAFQGEYFAARYGR
                     AAVAQTPPIARMLRAGIPVGAGTDATRVANYNPWDSIYWLVSGRTVGGFELYPEENRV
                     SRMEALRLYTVGSAWFSNEQAKKGAIVAGQLADLVVLSEDFFAIPEERIKRLESVLTM
                     LGGTVVHATEVFADFAPSELP"
BASE COUNT          359 a          588 c          636 g          369 t
ORIGIN      
        1 acaacgcgat tgggcgcggt cggcgaccta cgatgcgacc aaccagatcg tgaaggatca
       61 cgccggcggg tatggtctcg gagtcatcta cgccaaggcg atgtttggcg ggcaggaggg
      121 gaaataaaat tcgtttcggg aaaaacggag caccgcgaat tccatcccgc cggctttctc
      181 ccgacgttgt catgaatacc actcgccgcg aattcgtgcg ccgctcggcc gcgctcgccg
      241 cgtataccgc ggcgttcggc accttgcgcg gcgaggcttc acgccggacc gacgacaccg
      301 gcccggatcg tgagccgcaa tggattctgt ataacggcaa gatcgcgacc tgtgacgaaa
      361 cgcgaccctt cgcggaggcg gtcgcgattc gcgacggccg ctttctcgcg gtcggtgcga
      421 gcgattttat cctgcaactt ggcgggccat cgactcgcgt tgtcgacctc gcgggccgca
      481 ccgcgattcc cggactcaac gactctcacc tgcacgtcat ccgcggcggg ttgaattaca
      541 atctcgagtt gcgctgggac ggagtgccgt cgctcgcgga tggattgcgc atgttgcgtg
      601 agcaggcggc gcggacgccg gcgccgcaat gggtgcgtgt tgtcggcggg tggagtgagt
      661 ttcagtttgc ggagaagcgt ctgccgacgc tcgacgagat caacgaggct gcgccggaca
      721 cgcctgtttt catcctgcat ctctactgtc aggcgatgct gaaccgtgcg gcgttgcgtg
      781 cggttggcta cgacaagaac acgccgaatc ctccaggcgg cgagatccag cgggacaaga
      841 gcggcaatcc gaccggcctg cttcttgcgc gaccgaacgc ggcgatcctc tacgccacgc
      901 tggcgaaagg gccgaagttg ccgctcgact accaatacaa ttcgacgcgg cagttcatgc
      961 gcgagttgaa ccggctcgga gtgacgagcg tgatcgacgc cggcggcggc tttcaaaatt
     1021 acccggaaga ttatcaagtc atcgacgagc tgcaccgtcg cggcgagctc acgctccgca
     1081 tcgcctacaa tcttttcacg cagaaaccgc agcaggagaa agacgacttt gcgcgctgga
     1141 ttcggatggc gaagccgggg cagggcgacg acgtgttgcg actgaacggc gcgggcgaaa
     1201 tgctcgtgtt cagcgcggca gacttcgaag attttctcga gcctcgtccc gatctggcga
     1261 cgcggctcga aagcgatctg gaaggcgtga tctcgttgct cgcggcgaat cgctggccgt
     1321 ttcgcctgca tgcgacgtat gacgaatcga tcacacgttt cctgaacgtc atcgagaagg
     1381 tgaaccgggc ccatccgctc gacgggttgc actggtttct cgatcacgcg gagacgatcg
     1441 gcgaacgaaa catcgagcgc gtgcgcgcgc tcggcggcgg agtcgcggtg cagcatcgca
     1501 tggcgtttca gggcgaatac ttcgccgcgc gttatggccg tgctgcggtc gcgcaaacgc
     1561 ctccgattgc gcgcatgttg cgcgccggca ttccggttgg cgccggcacc gatgcgacgc
     1621 gcgtcgccaa ctacaatccg tgggactcga tctactggct cgtcagcgga cggaccgtcg
     1681 gtggattcga actttatccg gaggagaatc gtgtgagccg catggaagcg ctgcggctct
     1741 acaccgtcgg cagcgcctgg ttttccaacg agcaggccaa gaaaggcgcg atcgtggccg
     1801 gacaactcgc cgatctcgtg gtgctgtcgg aggatttttt cgcgatcccg gaagagcgaa
     1861 tcaagcggct cgaatccgtt ctgacgatgc ttggcggcac cgtcgttcac gcgaccgagg
     1921 tgttcgccga ttttgctcca tcggagttgc cc
//
LOCUS       DASPLT010001614         1175 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_163163, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001614 DASPLT010000000
VERSION     DASPLT010001614.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1175)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1175)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1175
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_163163"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1058)
                     /locus_tag="VEA63_16455"
     CDS             complement(<1..1058)
                     /locus_tag="VEA63_16455"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HYD85662.1"
                     /translation="MRVARWRWLAGATMRRARNLNAAWIALIWTVVLVGPLRAERVEL
                     VWPTPSKAWAEGRPAREFLQHAGSGEWESGGFGGVRSSGRQFHEGIDIKPVARNRRGE
                     PTDEIFAAMSGVVRHVSAVAGNSSYGRYIVIEHTELTPAVYTLYAHLAKIAPGVREGV
                     AVKRGQTIGEMGYTAGGYTIPKERAHLHFEIGLMVTRNFQTWYDARKFGSRNEHGVWN
                     GMNLMGIDPLDFYNAWRSRRVDTFEEYFAGMETAVRLRIATRRVPDFVERYPALLTKA
                     RPMLVGGWEIRFNWSGIPFAWTPLTTAEMDGLKLNEREIVAVDTKVERADRSKTLVVQ
                     RRGKWVVARDLETVLQQL"
BASE COUNT          199 a          471 c          287 g          218 t
ORIGIN      
        1 aacaactgct gcagcaccgt ctccagatcg cgcgccacga cccacttccc gcgccgttgc
       61 acgaccagcg ttttgctccg gtcggcccgc tcgaccttcg tgtccaccgc gacgatctcg
      121 cgctcgttca acttcaaccc gtccatctcc gccgtcgtca gcggcgtcca cgcaaacgga
      181 atcccgctcc agttgaaccg aatttcccag ccgccgacga gcatcggccg cgctttcgtc
      241 agcagcgccg gataacgctc cacgaagtcc ggcacgcgcc gcgtcgcgat ccgcagccgc
      301 accgccgttt ccatccccgc gaaatattcc tcgaatgtat ccacacgccg gctacgccat
      361 gcgttgtaga aatccagcgg atcgatcccc atcaggttca tcccgttcca gacgccgtgc
      421 tcgttccggc tgccgaattt ccgcgcgtca taccaggtct gaaaattccg cgtcaccatc
      481 aacccgatct cgaagtgcag gtgcgcgcgt tctttcggga tcgtataacc gcccgccgtg
      541 tagcccatct ctccgatcgt ctggccgcgt ttgaccgcaa cgccctcacg cacgcccggc
      601 gcgattttag caaggtgcgc atacaaggta tacaccgccg gcgtcagctc cgtgtgctcg
      661 atcacgatgt agcggccata gctgctgttt cccgcgactg cgctcacgtg tcgcacgacg
      721 cctgacattg cagcgaaaat ttcgtccgtc ggctcgccgc gtcgattccg cgccaccggc
      781 ttgatgtcga tgccttcgtg aaactgccgg ccgctgctgc gcacgccccc gaatccgccc
      841 gactcccatt cgcccgatcc cgcgtgctgc aaaaactccc gcgccggccg cccttccgcc
      901 caagccttgc tcggcgtcgg ccacaccaat tccacgcgct ccgcccgcag cggccccacc
      961 agcacgaccg tccagatcag cgcaatccac gccgcgttta aatttctcgc acgccgcatc
     1021 gtcgcgcccg ccagccaccg ccaccgcgcc accctcattt ctgcgcctcc tgcaatatcg
     1081 cgatcctcgc gcgcaaactt cccaccagcg ccggcagcgc ctcgtcgggc ttctccacga
     1141 aaatcagcag cgcgccgtcc tcaatcggtt gatcg
//
LOCUS       DASPLT010001615         1485 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_265976, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001615 DASPLT010000000
VERSION     DASPLT010001615.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1485)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1485)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1485
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_265976"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(6..>1485)
                     /locus_tag="VEA63_16460"
     CDS             complement(6..>1485)
                     /locus_tag="VEA63_16460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD85663.1"
                     /translation="DSVPNDPKTGLPIYFVSCSFNGPQFSSEADFKAGKTWDDWMNNP
                     ACFFAGAVQSLAVDFHGYTGERAYIDLVRQMLDHQLEFGTTPEGWPWAKVPYASADPG
                     NPVYQGATRWENAGMRGDGLHGIEPDKIGELGVGYFKFYQLTEEPKYLEAALHCADAL
                     AKHVKDTRIKGDDFSDAQAHRSPWPYRVNARSGVVIDEYTANVIEPIRLFEEILQRSD
                     RLDLSGEREKAYENAHRLAMNWLLSKSGPLITGVWNGYFEDVPSDPKLGNRVNNIPIE
                     TVRHFLKHPPKGLDVERHTLAQLAYVKSAFGEPGEPSINEQSWCYVPMGSHTARYASV
                     QAMMYERTGNAWHKREAEEHFNWATYCTATNGVVSVGPRWEGTWWSDGYGDYIKHFFD
                     GMAAVPEWAPANENHLLRSSSVVQTIAYTPAAITYRTYDTAGEEVLRLTARPKQIQLG
                     DRTLAERTDAADDAGWTWRALSEGGGVLKLRRTDDRDVKITL"
BASE COUNT          249 a          451 c          472 g          313 t
ORIGIN      
        1 cggtgctaca gcgtaatctt aacgtcgcgg tcgtcggtgc ggcggagttt cagcacgcca
       61 ccgccctcgg acagcgcgcg ccaggtccag ccggcatcgt cggcagcgtc ggtgcgttcg
      121 gcgagggtgc gatcgccgag ttggatctgc ttcgggcgcg cggtgagacg aaggacttct
      181 tcgccggcgg tgtcgtaggt gcgatacgtg atcgcggcag gcgtgtaggc gatggtttgc
      241 accacggacg acgagcgaag gagatgattt tcgttcgccg gagcccactc gggcacggcg
      301 gccatgccgt cgaagaagtg tttgatgtag tcgccgtatc catcgctcca ccacgtgcct
      361 tcccagcgcg gaccgaccga gacgacgccg tttgtcgcgg tgcaatacgt cgcccagttg
      421 aaatgctcct cggcctcgcg tttgtgccac gcgttgcccg tgcgctcata catcatcgct
      481 tgcaccgatg cataacgcgc ggtgtggctg cccatcggca cgtagcacca cgactgctcg
      541 ttgatcgacg gctcgcccgg ttcgccgaac gccgatttca catacgcgag ctgcgccaga
      601 gtgtggcgtt ccacatcgag gcctttcggc ggatgcttaa ggaaatgtcg caccgtctcg
      661 atcggaatgt tgttcacgcg gtttccgagc ttggggtcgg atgggacgtc ttcgaaatag
      721 ccgttccaca cgcctgtgat caacgggccg ctcttcgaca gcagccagtt catcgcgagc
      781 cggtgggcgt tttcataggc cttctcgcgc tcgccgctca ggtcaaggcg atcgctgcgc
      841 tgcaggattt cttcaaacaa ccggattggc tcgatcacgt tcgcggtgta ttcgtcgatg
      901 accacgccac tgcgcgcgtt cacgcggtaa ggccagggcg agcggtgcgc ttgtgcgtcg
      961 gagaaatcgt cgcctttgat ccgcgtgtcc ttcacgtgct tcgcgagggc gtcggcgcag
     1021 tggagcgcgg cttcgagata cttcggctcc tcggtcagtt ggtagaactt gaagtagccg
     1081 acgccaagct cgccgatttt atccggttcg atgccgtgca aaccgtcgcc gcgcatgccg
     1141 gcgttttccc agcgcgtggc gccttggtaa acagggttgc cgggatccgc gctcgcgtaa
     1201 ggcaccttcg cccacggcca tccttccggc gtcgtgccaa actcgagttg atgatcgagc
     1261 atctggcgca cgagatcgat gtaggcgcgt tcaccggtgt agccgtgaaa atccaccgcg
     1321 aggctctgca ccgcgcctgc gaagaaacac gcgggattat tcatccaatc gtcccaggtt
     1381 tttcccgcct tgaaatcggc ctcgctgctg aactgcgggc cgttgaacga gcatgagacg
     1441 aaataaatgg ggaggccggt cttgggatcg ttgggcacgc tatcc
//
LOCUS       DASPLT010001616         1056 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_59069, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001616 DASPLT010000000
VERSION     DASPLT010001616.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1056)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1056)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1056
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_59069"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..752
                     /locus_tag="VEA63_16465"
     CDS             <1..752
                     /locus_tag="VEA63_16465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD85664.1"
                     /translation="AVVASADAPAPSPALTPEVIEHYEDRAAGGDAIAAAILTEIYGR
                     SANTRDKALAFDYAYAAYGSGQPVGGAALGSAFSNGYGVEKDAALGDALLKQSGLDEL
                     LGAVLVGVAAREAFVRTDNSAGKVNAAIVMGTTAVGILNTIAQNQATDRFSRDPIRQT
                     RYRLNEMREDLTKRRPDNAGAVEHLAKLRNTLTAAAELPESHKAYLLERVSALETALA
                     SSPPDRNAALTIIEEIVPSRKAAATAQATSS"
BASE COUNT          209 a          422 c          275 g          150 t
ORIGIN      
        1 ccgcggttgt cgcctccgcc gacgcgcccg caccttcacc cgcgctcacg cctgaggtca
       61 tcgaacatta cgaagaccgc gccgcaggag gcgacgcgat cgccgcggcg attctcacgg
      121 agatctacgg ccgcagcgcc aacacgcgcg ataaggcgct cgccttcgac tacgcctacg
      181 ccgcttacgg cagcggccag cctgtcggcg gcgccgcgct cgggagcgcc ttcagcaacg
      241 gctacggtgt ggagaaggac gccgcgctcg gcgacgcgtt gctaaaacaa tccggcctcg
      301 acgaacttct cggcgccgtc ctcgtcggcg tcgccgctcg cgaagccttt gtccgcaccg
      361 acaactccgc cggcaaagtc aacgccgcca tcgtcatggg caccaccgcc gtcggcatcc
      421 tcaacaccat cgcgcaaaat caggcgaccg atcgtttctc ccgcgatcca atccggcaaa
      481 cgcgctatcg cctcaacgaa atgcgcgagg acctcaccaa gcgtcgcccc gacaacgccg
      541 gcgcagtcga acatctcgcc aaactccgca acaccctcac cgccgccgcc gaactgcccg
      601 agagccacaa ggcctatctc ctcgaacgcg tttccgcgct cgaaaccgcc ctcgccagtt
      661 cgcctcccga ccgcaacgcc gcgctcacca tcatcgagga aatcgttcct tcgagaaaag
      721 ccgccgcaac cgctcaagcc acgtcctctt aaacaaccct ccgcagccgc gcggccaagc
      781 cgtaaccatt ccgcgaacgt gtaggggcgc cgctcgtcgg cgcccgattc gccccccgac
      841 gcaaggtccg ccccaacact cactcatgag cagcgacacc cgcagagaaa ggttgaaccc
      901 agcgtgaatc gttccggcta agaacgactc gctctccgct cgcccgccgc gccgccaaac
      961 ttccggagat atttcaacgc gacctcgaac tcgtgcggca acggcgcact cagcgtcacc
     1021 ggctcgcgcg tcgtcggatg cttcaacgtc aactcg
//
LOCUS       DASPLT010001617         1639 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_59073, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001617 DASPLT010000000
VERSION     DASPLT010001617.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1639)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1639)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1639
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_59073"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1639
                     /locus_tag="VEA63_16470"
     CDS             <1..>1639
                     /locus_tag="VEA63_16470"
                     /inference="COORDINATES: protein motif:HMM:NF012686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation acetate symporter"
                     /protein_id="HYD85665.1"
                     /translation="ASDFFVAGRSVSVGWNASAISGEYLSAASFMGVAGMVMSSGYDA
                     LWYPVCYACGYLFLLLFIAGPLRRFGAYTIPDFAEGRYDSPLFRKIAVCFVLFIGFFY
                     TMPQMKGAGVTLAYIFPGMPYWGGVALVGAVITFNVALGGMKGITLVQAFQYWIKMFA
                     ISVPVFVLMSVYGFYNQQLAVNVAPGETPAALTAPAATGPNYNASLTREPLPANAPKD
                     ETWVSPFGPLTTKAVNAAAAILPAEERAAYVAEHQKPYSLLYTYSLIIALVCGTAGLP
                     HILVRFYTNPDGVAAKRTTMWVMILIGVFYVFPPIFGVIGRNLLPELYAGVGAKGTDK
                     IVLELPRIINERYGILGSILSGITCAGAFAAFMSTFSGLLVSMTGALAHDVYGRMLRP
                     KSSAAERLLAFKISAVIVGAVAIILGCFVEFLEINFMVGQAFAIAAASYFPLLFMSVW
                     WRGMTMKGAATGMLTGGLLALLAAALTNFSTLALDKGPMGKVFAGFAPLNTFWAHHPL
                     LRILCEQPAIWAVPLAITLMIVVSKATAAQVPGDVRMK"
BASE COUNT          258 a          600 c          430 g          351 t
ORIGIN      
        1 gccagcgatt tcttcgtcgc cggccgctcc gtctccgtcg gctggaacgc ctccgccatc
       61 tccggcgaat atctctccgc cgcatcgttc atgggcgtcg ccggcatggt catgtcctcc
      121 ggttacgacg ccctctggta tcccgtgtgc tacgcgtgtg gatacctttt tcttctcctg
      181 tttatcgccg gaccgctccg ccgcttcggt gcctacacaa tccccgactt cgccgaaggt
      241 cgctacgact cgcccctctt ccggaagatc gccgtctgct tcgtcctctt catcggcttc
      301 ttctacacga tgccgcagat gaagggcgcg ggcgtgaccc tcgcctacat tttccccggc
      361 atgccgtatt ggggcggcgt cgcactcgtc ggcgcggtga tcaccttcaa cgtcgcgctt
      421 ggcggcatga agggcatcac cctcgtgcag gctttccagt attggataaa aatgttcgcg
      481 atcagcgtgc ccgttttcgt gctgatgtcg gtttacgggt tctacaacca gcagctcgcg
      541 gtcaatgtcg ctcccggtga aactcccgca gccctcaccg caccggccgc aaccggtcca
      601 aactacaacg cctcgctcac ccgcgagccg ctccctgcca acgccccgaa ggacgaaacg
      661 tgggtctccc ctttcggccc gctcaccacg aaagccgtca acgccgccgc cgcgattctc
      721 ccggccgaag agcgcgctgc ctatgtcgcc gaacaccaga agccctactc gctcctctac
      781 acctattcgc tgatcatcgc gctcgtctgc ggcaccgccg gcctcccgca catcctggtt
      841 cgcttctaca cgaatcccga cggcgtcgct gcgaaacgca ccacgatgtg ggtcatgatt
      901 ctcatcggcg tgttctacgt tttcccgcct atcttcggcg taatcggtcg caatctcctg
      961 cccgaactct acgccggcgt cggcgcgaag ggcaccgaca agatcgtcct cgaactcccg
     1021 cgcatcatca acgagcgcta cggcatcctc ggctcgatcc tcagcggcat cacctgcgcg
     1081 ggcgcgttcg ccgcgttcat gagcacgttc agcggattgc tggtttcaat gaccggtgcg
     1141 ctcgcccacg atgtttacgg ccgcatgctg cgcccgaaat ccagcgccgc agaacgcctg
     1201 ctcgcattca aaatctctgc cgtgatcgtt ggcgccgtgg cgatcatcct cggctgtttc
     1261 gtcgaattcc tcgaaatcaa tttcatggtc ggccaggcct tcgcgatcgc cgccgcgagt
     1321 tattttccgc tgctcttcat gagtgtgtgg tggcgcggca tgacaatgaa aggcgccgcc
     1381 accggcatgc tcacgggcgg tctcctcgcg ctgctcgccg ccgcgctcac caacttctcc
     1441 acgctcgcac tcgacaaggg ccccatgggc aaagtcttcg ccggcttcgc cccgctcaac
     1501 acgttctggg cgcatcaccc gttgctccgc atcctctgcg aacaacccgc gatctgggcc
     1561 gttccgctcg cgatcacgtt gatgatcgtc gtctcgaagg cgaccgccgc ccaagtgccc
     1621 ggcgatgtcc gcatgaaaa
//
LOCUS       DASPLT010001618         2756 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_224209, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001618 DASPLT010000000
VERSION     DASPLT010001618.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 2756)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2756)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2756
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_224209"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..569)
                     /locus_tag="VEA63_16475"
     CDS             complement(<1..569)
                     /locus_tag="VEA63_16475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD85666.1"
                     /translation="MVWEWTQLALLVANLAWTTFCLGGYRAETMVASSALTGALFAVH
                     LAGWAGGWSASSSTRLNATKFHPAGWLFVPFVIYAAVNVARISPVPWIGWRDWLGWVQ
                     MIAVFWVVLNGVRSRATRATLGWSLVAIGFAAVLLACYQRFVQPDWLAMGRTQADQFL
                     GRASGPFGIPNSLAALLLLLLPPMIGRAF"
     gene            complement(770..1801)
                     /locus_tag="VEA63_16480"
     CDS             complement(770..1801)
                     /locus_tag="VEA63_16480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007360224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase beta-grasp
                     domain-containing protein"
                     /protein_id="HYD85667.1"
                     /translation="MAKYKLEYIWLDGYTPLASLRSKTQIKDYASFPKLEDLPLWGFD
                     GSSTQQAEGKSSDIVLKPVAVYPDSTRKNGVLVMCETYLHTGEPHPSNSRATIPDDAD
                     TWFGFEQEYFFFKDGAPLGFPTGGFPSPQGPYYTGVGFKNVGSVAREIVEKHIDLCLD
                     AGINIEGINAEVAKGQWEFQIFGKGSKSAADQMWVARYLLTRLTESYGIDIEWRCKPI
                     LGPFNQALDWNGSGMHSNFSTKHMREVGGKAYFEKLMAAFNKHMDEHIAVYGPENHLR
                     LTGLHETQSIDKFTYGVADRGASIRVPHSFVKADYKGYLEDRRPNSAADPYLVAGRIL
                     KTILTVPTS"
     gene            2184..2600
                     /locus_tag="VEA63_16485"
     CDS             2184..2600
                     /locus_tag="VEA63_16485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012377354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HYD85668.1"
                     /translation="MHEIKDTARALVRIGYDGRVHKTFRGHLAKERFEHEVKVLQRLE
                     ERGCTFVPKLLDVDPEHLKIITTHCGGRVDQLNPERQKEIFAELENYGVRHEDRELRN
                     ITYRISDGRFCIIDFEFATLLEDGADGVSLKPTIAQ"
BASE COUNT          574 a          941 c          770 g          471 t
ORIGIN      
        1 tgaaaagccc tgccgatcat cggcggcaac aacagcagca acaacgcggc gagactgttc
       61 ggaatgccaa acggcccgct cgctcgcccg agaaattgat ccgcctgcgt gcgccccatc
      121 gcaagccagt cgggctgcac gaaccgctga taacacgcga gcaacacggc ggcgaaaccg
      181 atcgccacga gcgaccaccc cagcgtcgct cgcgtcgcgc gcgagcgcac gccattcagc
      241 acgacccaga acaccgcgat catttgcacc caacccagcc agtcgcgcca gccgatccac
      301 ggcaccggcg aaatccgcgc aacgttcacc gccgcataga tcacgaacgg cacgaacagc
      361 catcccgcag gatgaaattt cgtcgcgttc aaacgcgttg aagacgacgc gctccacccg
      421 cctgcccagc cggccagatg caccgcgaac agcgcgccgg tcaacgcact cgacgccacc
      481 atcgtctccg cgcgatagcc accgaggcaa aacgtcgtcc acgcgaggtt cgcgacgagc
      541 agcgcaagct gcgtccactc ccaaaccaca gcgcgcgtat tcgcgttaaa attgaagcgg
      601 aaaaaatcca aggtgggccc gacctccgcg cgggccgttg gcgcgcctcg acacggaccg
      661 cccggagatc gatccctacc atgcgatcgc gtccgccgcg tccgagcaaa aaaaacagcg
      721 ccacgtcctc gcggacgtgg cgctgcttac gttgagctgg ctggaggcct tagctcgtcg
      781 gcacggtcag aatcgttttc agaatccggc cagcgacgag atacggatcg gcggccgagt
      841 tcggacgacg gtcctcgagg tatcccttgt agtcagcttt gacgaagctg tgcgggacgc
      901 gaatcgaggc accgcgatcg gcgacgccat aggtgaattt gtcgatcgac tgagtctcgt
      961 ggagacccgt gaggcggagg tgattctccg gcccgtagac ggcgatgtgt tcgtccatgt
     1021 gcttgttgaa agcggccatg agtttctcga agtaggcttt gccgccgact tcgcgcatgt
     1081 gcttggtcga aaaattcgag tgcatgccgg agccgttcca gtcgagcgcc tggttgaagg
     1141 gcccgaggat cggtttgcag cgccattcga tatcgatgcc gtagctctcg gtcaaacgcg
     1201 tgaggaggta gcgggcaacc cacatctggt cggcggcgct cttcgagcct ttgccgaaga
     1261 tctggaactc ccactggccc ttcgcgactt cggcgttgat gccttcgatg ttgatcccgg
     1321 cgtcgaggca caaatcgatg tgcttctcca cgatctcacg cgccaccgag ccgacgttct
     1381 tgaaaccaac acccgtgtaa tacggaccct gcggcgaagg aaagccaccg gtcgggaaac
     1441 cgagaggagc gccgtccttg aaaaagaaat actcctgctc aaaaccgaac cacgtatcgg
     1501 catcgtctgg aattgtcgcc cgcgaattgc tcgggtgcgg ctcgccggtg tggagatacg
     1561 tctcgcacat cacgagcacg ccattcttgc gcgtcgaatc cggatacacc gccaccggct
     1621 tcagcacgat gtcggagctc ttgccctccg cctgctgggt ggaactgccg tcgaagcccc
     1681 agaggggaag atcttcgagc ttggggaagc tggcgtagtc cttgatttga gtcttgctgc
     1741 gcaagctggc caagggcgtg tagccatcga gccagatgta ttccagtttg tatttggcca
     1801 tagaggagtc ggattggagt cgagtttttt ggggggctct cgccgtgatc gtcaagtcgg
     1861 gcatccacgc ccaagctcgg caaacacaga caaagagccg tccccggaat tagcacgacg
     1921 tatgccagag gcgatcagaa cttggccggc ttatcgcctt atgacgcgcg gcctctccaa
     1981 ctgtgggagg ggctttacgc cccgactccg cgtcgcccaa accaaaccat ttgcaaactc
     2041 ccctgcgcgc acgtcgctcg tcaacgccca caccccgtgc tcaccacctt cattcgcgtc
     2101 gcttcgcgcc cattcgcggg tctccgcctc gccgcacaac cgcgcttgcc acctttcgcg
     2161 cggtgaaatc aggttagcgc cacatgcacg aaatcaaaga caccgcccgc gcgctcgtgc
     2221 ggatcggcta cgatgggcgg gtgcacaaaa cgttccgcgg ccacctcgcc aaggaacgct
     2281 tcgaacacga agtgaaagtc ctccaacgcc tcgaagaacg cggctgcacc ttcgtcccca
     2341 agctcctcga cgtcgatccc gagcacctca aaatcatcac cacccactgc ggcggccgcg
     2401 tcgaccaact caacccggag cgccagaaag agatctttgc agaactcgaa aactacggcg
     2461 tccggcacga ggaccgtgag ttgcgcaaca tcacttaccg catctccgac ggacgcttct
     2521 gcatcatcga cttcgagttc gccacgctcc tcgaagacgg cgccgacggc gtctcgttaa
     2581 aaccgaccat cgctcaatga gcacccccga cggctccgcc gccaccccgc ctccgccggc
     2641 cgaaacgccg ccccctggct ccacccgcgt ccgctggtcc ggcctcaccc atgtcggccg
     2701 ctaccggccc aacaacgaag atgcctttct cgcgctcaac ttcgacggcc acgatc
//
LOCUS       DASPLT010001619         1961 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_59142, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001619 DASPLT010000000
VERSION     DASPLT010001619.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1961)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1961)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1961
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_59142"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..350
                     /locus_tag="VEA63_16490"
     CDS             <1..350
                     /locus_tag="VEA63_16490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ThuA domain-containing protein"
                     /protein_id="HYD85669.1"
                     /translation="LGDELKVQEEWYTLKEFARDLHVQLVMETAGMEGSDYQRPPYPI
                     AWARLHGKGRVAYNAMGHREDVWDSEAFQSMLVGALKWAGGLVDADVTPNLEQVAPGH
                     ATIQPQPPQAPKK"
     gene            complement(416..>1961)
                     /gene="ccsA"
                     /locus_tag="VEA63_16495"
     CDS             complement(416..>1961)
                     /gene="ccsA"
                     /locus_tag="VEA63_16495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HYD85670.1"
                     /translation="DDHKRFSFSQLQKSLPELERQATLARAVESAVRTPFQKAVVQLH
                     GNLLRYQQLQYSLVSPGSTDFLGELTRFHSALEKGVAAIRAKQAGQPHDEAAAQLMID
                     SGQRYIQMSQFGLLLAVPPVSGEGQLDGWKSVGSALLETFQTAQMNPYAMTYAGLART
                     WQTGQAKEFNQIVTLYRAQLEKSFQTTLSKTGAEQRFNAAEPFYKSMNLYVLAFLLAL
                     FSWLKWPEALGRSAFWLVALAWLISTVGIGTRMWLEGRPPVTNLYSSALFVGWGSVAL
                     CLVLEAVYRNAIGSVAAAMIGFATLLIAHHLSLGGDTLEMMRAVLDSNFWLATHVVVV
                     AIGYSATALAGFLAIIYVVRGVFTRSLDQATAGSLARMVYGIICFATLFSFVGTILGG
                     IWADQSWGRFWGWDPKENGALMIVIWNAIILHARWGGLVRQRGLMSLAIFGNVIFSWS
                     WFGTNMLGVGLHSYGFTDAAFVALIGFVLSQVALIAIANLPLEKWRSFRTTPAPTAPE
                     HVEVLN"
BASE COUNT          386 a          565 c          684 g          326 t
ORIGIN      
        1 agctcggcga tgagctcaag gtgcaggagg agtggtatac gttgaaggag ttcgcgcggg
       61 atctgcatgt gcagctcgtg atggaaaccg ccggcatgga aggcagcgac tatcagcggc
      121 cgccgtatcc gatcgcgtgg gcgcgtttgc acggcaaagg acgcgttgcg tataacgcga
      181 tggggcatcg cgaggacgtg tgggacagcg aggcgtttca gtcgatgctg gtgggcgcgc
      241 tcaagtgggc gggcggcctg gtcgacgcgg acgtgacgcc gaacctcgag caggtcgcgc
      301 cgggccatgc gacgattcag ccgcagccgc ctcaggcgcc gaagaagtag gtcggcgagg
      361 gccgcgatct aggcggcgcg aagcggcggg gtcgggagac cccgccctac atactttagt
      421 tcagcacttc gacgtgctcg ggcgccgtgg gcgcgggcgt cgtgcggaag ctgcgccatt
      481 tttcgagcgg caggttggcg atcgcgatga gcgccacctg gctcaacaca aaaccgatca
      541 gcgcaacgaa ggcggcgtcg gtaaatccgt agctgtggag cccgacgccg agcatgttcg
      601 tgccgaacca gctccagctg aaaatcacgt tgccgaagat tgcgaggctc atgaggccgc
      661 gttggcggac gaggccgccc cagcgggcgt ggagaatgat cgcgttccag atcacgatca
      721 tgagcgcacc gttttccttc gggtcccagc cccagaagcg gccccacgat tgatcggccc
      781 aaatgccgcc gaggatcgtt ccgacaaagc tgaacaaggt cgcgaagcaa atgatgccgt
      841 agaccatccg cgcgagactc cccgccgtcg cctgatcgag cgaacgcgtg aacacgcctc
      901 gcacgacgta gatgatcgcg agaaacccgg cgagcgcggt cgccgagtag ccgatggcga
      961 cgacaacgac gtgcgtcgcc agccagaagt tcgagtcgag cacggcgcgc atcatttcga
     1021 gcgtgtcgcc gccgagggaa aggtgatgcg cgatgagcag cgtcgcaaag ccgatcatgg
     1081 cggcggcaac gctgccgatc gcgttgcgat aaaccgcttc gagcacgagg cagagcgcga
     1141 cggaacccca gccgacaaac agcgcggatg aataaagatt cgtgacgggc ggacggccct
     1201 cgagccacat gcgcgtgccg atgccgacgg tggagatcaa ccacgcgagt gcgaccagcc
     1261 agaacgcgga gcggccgagc gcctcgggcc atttgagcca ggagaacagc gcgagcaaaa
     1321 acgcgagcac gtagaggttc atgctcttgt agaacggttc ggcggcgttg aagcgctgct
     1381 cggcgccggt tttgctgagc gttgtctgga aagatttttc gagctgcgcg cggtagaggg
     1441 tgacgatctg gttgaattct ttcgcctgac cggtctgcca cgtgcgagcg aggccggcgt
     1501 aggtcatggc gtaaggattc atttgcgcgg tctggaacgt ttcgagcaac gcggacccca
     1561 cgcttttcca accgtcgagc tggccctcgc ccgagaccgg tggaaccgcg agtaacaatc
     1621 cgaactggct catttggatg tagcgttggc cggagtcgat catcagttgc gcggccgctt
     1681 catcgtgcgg ctggccggct tgcttcgcgc gaatggcggc tacgcctttt tccaacgccg
     1741 agtggaagcg tgtcagttcg ccgagaaagt cggtgctgcc gggcgacacg aggctgtatt
     1801 gcagttgctg ataccgcagg aggttgccgt gaagctgcac gaccgccttc tggaaagggg
     1861 tgcgaacggc ggactccacc gcgcgggcga gcgtcgcctg gcgttcgagt tccggcagcg
     1921 atttctgcag ctgggaaaac gagaagcgct tgtgatcgtc g
//
LOCUS       DASPLT010001620         1341 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_163259, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001620 DASPLT010000000
VERSION     DASPLT010001620.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1341)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1341)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1341
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_163259"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1341)
                     /locus_tag="VEA63_16500"
     CDS             complement(<1..>1341)
                     /locus_tag="VEA63_16500"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF012630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031012 - extracellular matrix [Evidence
                     IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="matrixin family metalloprotease"
                     /protein_id="HYD85671.1"
                     /translation="QLGRPPSALIDGATSWDDIAVAALETWNPVLQRSQFGYVKSSNV
                     SKSRGNGYNNVFFDSTIYGQSFGDRVLAVTLMRSTGGRLVETDVVVNTANTWNSYRGN
                     RGSRSSVDLQRVLVHEFGHALGLDHPDQATPPQNVAAIMNSMITNIYTLQNDDIAGAQ
                     SLYGPSPAPLPGPGTLTSQSVNAGAPLTIALANPNPNYTQYAWTFTRPGGSPATLLDG
                     DGNPWTAPTYRLFSAQPTDTGTYAVAAVNGLALTDPATATVEVKPVSTAGSRLANLSA
                     RARAGSGDDTFIVGFVIGGKTEKRVLIHALGPALSGAVSQPLGNPRLRLLRHTGNGVF
                     APVAENDDWSADATDASALRAAAQRLGAAPLPSNSRDAALLATLPPGVYSAQVDAQGG
                     TPGIALVEVYDADADTTAALPRRLMNISSRSFAGSGEQTLIAGFVVDGSSPKQVL"
BASE COUNT          226 a          393 c          478 g          244 t
ORIGIN      
        1 cagcacctgt ttcgggctcg agccgtcgac cacgaaaccg gcgatcagcg tttgttcacc
       61 gctgccggca aagctgcgcg aggagatatt catcaatcgc cgcggcagcg cggccgtcgt
      121 gtccgcgtcg gcatcgtaga cttcgacgag cgcgataccc ggtgtgccgc cttgcgcgtc
      181 aacctgcgcg gaataaacgc ccggagggag cgtcgcgaga agcgcggcgt ctctcgagtt
      241 cgacggcagc ggtgcggcgc cgagacgttg cgccgccgcg cggagggccg aggcatcggt
      301 ggcatcggcg ctccagtcgt cgttttcagc gacgggggcg aagactccgt tgccggtgtg
      361 gcggagcagt cgcaaacgcg gattcccgag cggctgactc accgcgcccg acagagcggg
      421 accgagcgcg tggatgagca cgcgtttctc ggttttaccg ccgatcacga aaccgacgat
      481 gaatgtatca tcgccggagc cggcgcgggc gcgcgcggag agatttgcca gacgtgagcc
      541 cgcggtgctc acgggtttga cttctacggt tgccgtcgcg ggatcggtca gcgcgaggcc
      601 gttcaccgcc gcgaccgcat aggtgccggt atcggtcggt tgagcggaaa agagccggta
      661 agttggcgcg gtccacggat tgccgtcgcc gtcgagcagc gtcgcaggcg agccgcctgg
      721 ccgggtaaac gtccacgcat actgggtgta gttgggattt ggattcgcga gcgcgatggt
      781 gagcggtgcg ccggcgttga cgctctggct ggtgagggtt cccgggccgg ggaggggagc
      841 cggactgggg ccgtagagtg actgggcgcc ggcaatgtcg tcattctgca acgtgtaaat
      901 gttcgtgatc atgctgttca tgatcgcggc cacgttttgc ggcggagtgg cctggtccgg
      961 atgatcaagg ccgagtgcat gaccgaactc atggacgagc acccgttgca gatcgaccga
     1021 gctgcgtgag ccgcgatttc cgcggtagga gttccaggtg ttggccgtgt tgaccaccac
     1081 gtcagtttcg acgagccgcc cgccggtgct gcgcatcaac gtgaccgcca gcacccgatc
     1141 gccgaaggac tggccgtaga tggtggaatc gaagaagacg ttgttgtatc cgtttcctcg
     1201 cgacttcgaa acattcgagc ttttgacgta gccgaactgg ctgcgctgaa gcacgggatt
     1261 ccaggtctcg agcgctgcca cggcaatgtc atcccaactg gtggcgccgt cgatcagcgc
     1321 agatggcggc cggccgagtt g
//
LOCUS       DASPLT010001621         1203 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_163261, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001621 DASPLT010000000
VERSION     DASPLT010001621.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1203)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1203)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1203
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_163261"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1148
                     /locus_tag="VEA63_16505"
     CDS             <1..1148
                     /locus_tag="VEA63_16505"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HYD85672.1"
                     /translation="QLARLLHAPIAKALLGKDVLPDDSPYTTGTIGVFGTSATAAVMK
                     EADTLLLIGTSFPYISYLPDPSRVRGVQIDINPERIALRFPVEVGLVGDSAETLRVLI
                     PLIEQHHDSRLLDLAREKMTEWWQLMERRGTSRDLPMRPQTVAWELGKRLRDDAIICG
                     DSGQNTLYVARQIKIRGTQRFSCSGMLASMGCALPYAIGAQIAFPARQVIAFAGDGGL
                     TMVLGELATCVKHQLPIKIFVLKNNTLGMIRWEQMMFLGNPEYGTELQNIDFGRVAEA
                     FGLRAFHVEQPVDLADAIDAALAHPGPVLVEATIDPDEPLMPGQIKPEQAENYACALK
                     NGQPNARRIALTLFRDAVEDLGENRRTIPEQLREKVPELTPRETRKA"
BASE COUNT          243 a          389 c          356 g          215 t
ORIGIN      
        1 aacaactcgc ccgcctgctg cacgcgccga tcgcgaaagc actgctcggg aaagacgtgt
       61 tgccggatga ctcgccttac accacgggca cgattggcgt gttcggcaca tcagcgaccg
      121 ctgccgtgat gaaggaagcg gatactctcc tgctgatcgg cacgtcgttt ccttacatca
      181 gctacctgcc cgatccgagc cgcgttcgcg gcgtgcagat cgacatcaat ccggaacgca
      241 tcgcgctgcg cttcccggtg gaagtcggcc tcgtgggcga ctcggcggaa acgctgcggg
      301 ttttgattcc attgattgaa caacaccacg attcccgcct cctcgacctc gctcgcgaga
      361 aaatgaccga gtggtggcag ctcatggaac gtcgcggcac gtcgcgcgac ttgccgatgc
      421 gtccgcaaac ggtcgcatgg gaactcggga aacgcctgcg cgacgacgcg atcatctgcg
      481 gcgactccgg gcaaaacacg ctctacgtcg ctcgccagat caagatccgc ggcacgcagc
      541 gattctcctg ttccggcatg ctcgcgtcga tgggctgcgc attgccctac gcgatcggcg
      601 cgcaaatcgc gtttcccgcg cggcaggtga tcgcgttcgc cggagacggc ggtctcacca
      661 tggtcctcgg cgagctcgcc acctgcgtga agcaccagct tccgatcaaa atcttcgtgc
      721 tgaagaacaa cacactcggg atgatccgct gggagcagat gatgtttctc ggcaatccgg
      781 aatacggcac ggaactgcag aacatcgatt tcggccgcgt cgccgaagcg ttcggcctgc
      841 gcgcttttca cgtcgaacaa cccgtggatc tcgccgatgc gatcgatgcc gcgctggcgc
      901 atcccggtcc agtgctggtt gaggccacga ttgatcccga cgagccgctg atgcccgggc
      961 agatcaaacc ggagcaggcc gaaaactacg cttgtgcgct caaaaatggc caacccaacg
     1021 cacgccggat cgcgctcacg ctctttcgcg atgccgtgga agatctcggc gaaaaccgcc
     1081 gcacgattcc ggagcaactg cgcgagaaag tcccagaact cactccgcgt gagacgcgca
     1141 aggcgtgacc acatctgcgg agttatccga gatcggaatc tggtggggct tactgccgaa
     1201 gaa
//
LOCUS       DASPLT010001622         1563 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_163275, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001622 DASPLT010000000
VERSION     DASPLT010001622.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1563)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1563)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1563
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_163275"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1563
                     /gene="acs"
                     /locus_tag="VEA63_16510"
     CDS             <1..>1563
                     /gene="acs"
                     /locus_tag="VEA63_16510"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007363413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HYD85673.1"
                     /translation="ATYKRLYAESVNSPEIFWAKQAKEHLVWRKPFKKTLKWKAPHAE
                     WFVGGKLNVAENCLDRHLGTARENKAALIFEGEPGDVRTITYKQLHFHVCRLAHIFEN
                     MGIVAGDRVAIYMPMIPEAVMAMLACARIGAIHTVIFGGFSAEAIKDRLNDCQAKLVI
                     TADGGWRRGKVIELKANVDKALEGAPTVHTVMVVKRCGNPINMVEGRDVWWKEAWEGA
                     PNTHKAKAFDSEHPLFILYTSGSTGKPKGVMHTSAGYLLGAKLSSHYVFDLKETDRYF
                     CSADIGWITGHSYVVYGLMSNGATIFIYEGAPNQPEPDRFWQMIDRHGITILYTAPTA
                     IRAFMRWGDNYVLRHRLDSLRLLGSVGEPINPEAWMWYHKMIGKKRCPIVDTWWQTET
                     GTIMIAPMPGITPLKPGSATRPFFGVVPKVVDDNGKEVPRNSGGKLIITKPWPSMLRT
                     LWGDDERYQKAYWSEFKGVYFTGDGARVDDDGYFWLLGRVDDVLNVAGHRLGTMEVES
                     ALVDHPHVAEAA"
BASE COUNT          329 a          426 c          519 g          289 t
ORIGIN      
        1 atgccactta caaaaggctc tacgcggagt ctgtcaactc cccagagata ttctgggcga
       61 agcaggcgaa ggagcatctc gtgtggcgca agccgttcaa gaaaacgctg aagtggaaag
      121 cgccgcatgc cgagtggttc gttggcggaa agctcaacgt ggcggaaaac tgcctcgatc
      181 gtcatctcgg cacagcgcgc gaaaacaaag ccgcgctgat tttcgaaggt gagccgggcg
      241 atgtgcgcac gatcacttac aagcaactcc actttcacgt gtgccggctc gcgcacattt
      301 ttgaaaacat ggggatcgtc gccggagatc gcgtggcgat ctacatgccg atgattccgg
      361 aggcggtgat ggcgatgctc gcgtgcgcgc gcatcggtgc gattcacacg gtgattttcg
      421 gcgggttttc cgccgaagcg atcaaggacc ggttgaacga ttgccaggcg aaactcgtca
      481 tcaccgcgga cggcggctgg cggcgcggca aggtgatcga gctcaaggcg aacgtcgaca
      541 aggccctcga gggcgcgccg accgtgcaca cggtgatggt cgtgaaacgt tgcggaaacc
      601 cgatcaatat ggtcgagggc cgcgacgtct ggtggaagga agcgtgggag ggcgcgccga
      661 acacgcacaa ggcgaaggca ttcgattcgg agcatccgct gttcattttg tatacaagcg
      721 ggtcgaccgg gaaaccgaaa ggcgtgatgc acacgagcgc gggctatctg ctcggcgcga
      781 aactgagttc gcactacgtt ttcgacctca aggaaaccga ccgctatttc tgctcggcgg
      841 atatcggttg gatcacgggc cacagctatg ttgtctacgg cttgatgtcg aacggcgcga
      901 cgatcttcat ctacgaaggg gcgccgaacc agccggagcc cgatcgtttt tggcagatga
      961 tcgaccggca cggtatcacg attttgtata cggcgccgac ggcgatccgg gcattcatgc
     1021 gctggggcga caactatgtg ctgcggcacc gactggattc gttgcgcctg ctcgggtcgg
     1081 tgggcgagcc gatcaatccc gaggcgtgga tgtggtatca caaaatgatc ggcaagaaac
     1141 gctgtccgat cgtcgacacg tggtggcaga cggaaaccgg cacgatcatg atcgcgccga
     1201 tgccaggcat cacgccgttg aaaccgggtt cggcgacgcg gccgtttttc ggcgtggtgc
     1261 cgaaggtcgt cgacgacaac ggcaaggaag tgccgcgtaa ttcgggcgga aaacttatca
     1321 tcaccaagcc gtggccgtcg atgttgcgca cgctgtgggg cgacgatgag cgctatcaga
     1381 aagcgtattg gagcgagttc aagggcgtgt atttcaccgg cgacggtgcg cgcgtcgacg
     1441 atgacgggta cttctggctg ctaggccgcg tagacgacgt gctgaacgtc gcggggcacc
     1501 gcctcggcac catggaagta gagagcgcgc ttgtcgatca tccccacgtc gccgaggcgg
     1561 ccg
//
LOCUS       DASPLT010001623         1615 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_204427, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001623 DASPLT010000000
VERSION     DASPLT010001623.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1615)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1615)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1615
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_204427"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..255
                     /locus_tag="VEA63_16515"
     CDS             <1..255
                     /locus_tag="VEA63_16515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD85674.1"
                     /translation="QTAWLPPRDLEPGDYEAVFTQDRVVIEAGTYVLRIGLSSTERTF
                     QQFDATRIDIVSEGAKGFFPVTSGVGLVLNSMETSLVRTA"
     gene            252..536
                     /locus_tag="VEA63_16520"
     CDS             252..536
                     /locus_tag="VEA63_16520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD85675.1"
                     /translation="MRPVSTAGHPPRWRRIFQKAALRTRLAWQCLTSFEDTVSRSTLQ
                     VSYAQNGEDLIAWHALHSLGVREPRYLDIGAHHPVRLSNTALFHLLGAAA"
     gene            533..1024
                     /locus_tag="VEA63_16525"
     CDS             533..1024
                     /locus_tag="VEA63_16525"
                     /inference="COORDINATES: protein motif:HMM:NF016908.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HYD85676.1"
                     /translation="MNIEPDPVLFEAFPRHWPRDINLNVGVGPTAGEMVFFRMANPLL
                     NTFSAEEAERIARDEGIPIASRTRVRIENITTLLDRWDFTPDFLTIDTEGHDLLILQS
                     YDFTRRRPAVICVETLTFSTQSRGRKLPEITECLLRVGYRVHADTQINTLFVDGSRMP
                     SSR"
     gene            1053..>1615
                     /locus_tag="VEA63_16530"
     CDS             1053..>1615
                     /locus_tag="VEA63_16530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD85677.1"
                     /translation="MIVTRLTGGLGNQMFQYAAGLALAERRRTVLKLDVSWFREYAEY
                     EAHNRYALSCFNITEQFATQEEIDRVRGVALTRTERWSIMLARRLRLYRYANRHAAPS
                     NLHRAQGTAFYPAFFDQPDNTMLEGMWQSELFFAPIQHLVRLHFSFRYPPQPAVEAVA
                     ERIRADGPSIAVHFRRGDYARNATFSR"
BASE COUNT          275 a          586 c          443 g          311 t
ORIGIN      
        1 cagaccgcct ggctgccgcc gcgcgatttg gaacccggtg attacgaggc cgtgttcacc
       61 caggatcgcg tcgtgatcga ggccggcacc tatgtgctcc gcatcggcct ctccagcacc
      121 gaacgcacct tccagcagtt cgacgccact cgcatcgaca tcgtgagcga aggagccaaa
      181 ggatttttcc cggtcacgtc cggcgtcggc ctcgtgctga attccatgga aacgtcgttg
      241 gtgcgcacgg catgagaccg gtttcgaccg cgggccatcc gccgcgctgg cgccgcattt
      301 ttcaaaaggc tgcgctccgc acgcgccttg cctggcagtg cctcacctcg ttcgaagaca
      361 cggtcagccg tagcaccctg caggtcagct acgcgcaaaa cggcgaggat ctcatcgcgt
      421 ggcatgcgct gcactcgctc ggggtccgcg agccgcgtta cctcgacatc ggcgcgcatc
      481 acccggtgcg tctcagcaac accgcgctct tccatttgct cggcgccgcg gcatgaacat
      541 cgagccggac cccgtgctct tcgaagcttt cccacgccac tggccccgcg acatcaacct
      601 caacgtcggg gtcggcccca ccgcaggcga gatggtgttt ttccgcatgg cgaatccgct
      661 cctcaacacc ttttccgccg aagaagccga gcggatcgcg cgcgacgagg gcattcccat
      721 cgcgtcgcgc acccgcgtgc gcatcgagaa tatcactacg ctgctcgacc gctgggactt
      781 tacgcctgac tttctgacga tcgataccga gggccacgat ctgttgattc tgcagagtta
      841 cgacttcacc cgccggcgtc ctgcggtgat ctgcgtcgag accctcacgt tttcaaccca
      901 gtcgcgcggt cgcaagctgc ccgagatcac cgaatgtctc cttcgcgtcg gctaccgcgt
      961 gcacgccgac acgcagatca acacgctttt cgtcgacggc agtcgaatgc cttcttcccg
     1021 gtgaattcgc cgggccccac tgacctcctc tcatgatcgt cacccgactc accggcggac
     1081 tcggcaacca gatgtttcaa tatgcggctg gcctcgcgct cgccgagcgg cgtcgcacgg
     1141 tgttgaagct cgatgtgtcg tggtttcgcg aatacgccga atacgaggcg cacaaccgtt
     1201 acgcgctctc gtgcttcaac atcacggagc agttcgccac gcaggaggaa atcgatcgcg
     1261 tccgcggggt cgcgctcacc cgcaccgaac gctggtccat catgctcgcc cgccggctcc
     1321 ggctttaccg ctacgccaat cgccacgccg ccccctccaa cctccatcgg gcgcaaggca
     1381 ccgcgtttta tcccgccttc ttcgatcagc ccgacaacac gatgctcgag ggcatgtggc
     1441 agtccgagct cttcttcgcc ccaatccagc acctcgttcg attgcacttc agcttccgtt
     1501 atccgccgca acccgccgtc gaagccgtgg ccgagcgcat ccgcgcagat gggccttcga
     1561 tcgccgtgca ctttcgccgc ggcgattacg ctcgcaatgc gaccttcagc cgtga
//
LOCUS       DASPLT010001624         1089 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_100599, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001624 DASPLT010000000
VERSION     DASPLT010001624.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1089)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1089)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1089
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_100599"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1001)
                     /locus_tag="VEA63_16535"
     CDS             complement(<1..1001)
                     /locus_tag="VEA63_16535"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HYD85678.1"
                     /translation="MSAHEPNSLPHIVVVGAGFAGLTFCRKLPAGLARITMVDRQNHH
                     LFQPLLYQVATAGLSAVDIAQPIRSILRSVSNLEVLMAEVIGVDLVGRKLRLRSSTDR
                     VEEMDVSYDYLVLAVGGVTSYFGRDEWAQLAPGLKSLDDAIRIRRKILLAFERAEMEC
                     DEQRQRELMTILVIGGGPTGVELAGTFAELARTVLVRDFDRIDPRKAHVILVEGGDRV
                     LGTFPPDLSASAERQLARLGVEVRKNLRVQAIREGEAQLSDGETIRAANIIWGAGVAA
                     SPLTKLLGVELDRGGRVKVAPDLSLPGHPEAFALGDMVSLVDKNGRMVPGVSPAAIQ"
BASE COUNT          209 a          371 c          315 g          194 t
ORIGIN      
        1 atctggatgg ccgccggcga cacgccggga accatgcggc cgttcttgtc caccagcgag
       61 accatgtcgc ccagcgcgaa cgcctcgggg tggccgggca ggctgagatc cggtgcaacc
      121 ttcacgcgtc cgcctcgatc gagttccacg ccgaggagtt tggtcaacgg cgacgctgcg
      181 acgcccgcgc cccagatgat gttcgccgcg cgaatggttt cgccgtccga caattgggcc
      241 tctccttcac gaatcgcctg cacgcgcagg tttttgcgca cttcaacgcc gagccgcgcg
      301 agctgccgct cggcgctggc ggaaagatca ggcggaaacg tgcccagcac gcgatcgcca
      361 ccttcgacca gaatcacatg agccttgcgc ggatcgatcc ggtcgaagtc gcgcaccagg
      421 accgtgcgcg cgagttcggc gaaggtcccc gcgagttcga caccggttgg tcctccgcca
      481 atcacgagaa tcgtcatcaa ttctctctgg cgctgctcgt cgcattccat ttccgcccgc
      541 tcgaatgcca gcaggatttt tcggcgaatc cggatggcgt cgtcgagcga cttcaatcct
      601 ggcgccagtt gcgcccattc gtcgcgcccg aagtagctgg tgactcctcc caccgcgagc
      661 acaaggtagt cgtagctcac gtccatttcc tccacccgat ccgtggagct ccgcagtcgc
      721 agtttccgac cgaccagatc gacgccgatc acttcagcca tgagcacctc gaggttcgac
      781 accgatcgca ggatcgagcg gatcggctgc gcgatatcca ccgctgagag accggctgtc
      841 gccacctgat aaagcagcgg ctgaaagagg tgatgatttt ggcgatcgac catcgtgatc
      901 ctcgccaggc ccgccggtag tttccgacaa aacgtcagcc ctgcaaaacc ggcgccgacg
      961 accacgatgt gcggaagaga gtttggctcg tgagcagaca tgatcgcgta acggatacgc
     1021 aaacgctccg ttggcaacgc tctccgcgaa acatcgccac gccgttcgga aatcccgcac
     1081 cggcccaat
//
LOCUS       DASPLT010001625         2380 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_80388, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001625 DASPLT010000000
VERSION     DASPLT010001625.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 2380)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2380)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2380
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_80388"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(91..>2380)
                     /locus_tag="VEA63_16540"
     CDS             complement(91..>2380)
                     /locus_tag="VEA63_16540"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HYD85679.1"
                     /translation="HETAATHRFEIGDDDFLLDGNRFQIRCGELHAPRVPREYWQHRL
                     RMIKAMGLNTVCAYLFWNMHEPRPGEYDWSGQADVVEFCRAAQAEGLWVILRPGPYAC
                     AEWEMGGLPWWLLKHDDIKLRSRDPRYLEAAKRYLIEVGRVLAPLQVTHGGPILMVQV
                     ENEYGFYGKDAAYMGELRQALLDGGFDVPLFACNPKQHLRDGYRDDLFPVVNFGSDPA
                     GGFKALREILPTGPLMCGEFYPGWFDTWGAPHHTGNTERYLADLGYMLEVRASFSIYM
                     AHGGTTFGFFTGADRPFKPDTSSYDYDAPVSEAGWVTPKFEQTRALLAKHLLPGETLP
                     EPPARNPVIAISPVPATQVARVFSNLPEPLTDEHPRTMELYDQAFGCILYRTQLPAGP
                     AGTLEATAIHDIGQVFLNGERIGFTDRRSRNFRVQLPERSQPATLDILVEAMGRVNFG
                     VEVHDRKGIHGPVTFVVSGETVVELRDWQIFPLPLDAAMLDALRFDAAENADTAAVDA
                     PAFWRATVEIDQPGDCFLDMRPWGKGFVWVNGHNLGRYWNIGPQQTMYVPGPWLKPGA
                     NEIIILDLLGLETAVVAALDQPILNELRPALDFARSKRRDVHLRSDFGEAAHRGAFAP
                     GTSLQEVPFSKPTRGRYFCLEALEAHDGKPSAAVAEITLLDASGQPLSTQGWTIAYVD
                     SEEREREDGTAENAIDGQTANFWHTQWSAAQPGYPHRLILDLAETRTVSGFRYVPRQG
                     PPETPGRIKDYRIYVADDLIVP"
BASE COUNT          435 a          701 c          785 g          459 t
ORIGIN      
        1 ccgcgctttg acgagatccg tctcgtggtc ccctgtgttg accaccgctt gggaatggaa
       61 cccaggccga ttcagagacc tgcccgataa tcacgggacg atcagatcgt cggcgacgta
      121 gatgcggtag tctttgatcc ggccgggcgt ttcaggtgga ccttggcgcg gaacgtaacg
      181 aaagccgctg accgtgcgcg tttcagcgag gtcgagaatc aagcggtgcg gataacccgg
      241 ctgcgccgcg ctccactgcg tgtgccagaa gttggcggtt tgtccatcga tcgcattttc
      301 cgccgtgcca tcctcgcgct cgcgttcctc gctgtcgacg taggcgatcg tccaaccttg
      361 cgtgctcagc ggctgacccg acgcgtcgag cagcgtgatc tcagccacgg cggcggaggg
      421 cttgccgtcg tgcgcctcga gcgcttcgag gcagaaatat cgaccgcgtg ttggcttgga
      481 aaacggcacc tcctgcaaac tcgtgccagg ggcgaacgct cccctgtgcg ccgcttcacc
      541 aaaatcggag cgcagatgaa cgtcgcgacg cttgctgcgc gcgaagtcga gggcgggccg
      601 cagctcgttc aagatcggct ggtccagcgc agcgacaacg gcggtttcaa ggccgagaag
      661 atctaggatg atgatttcgt tcgctcccgg tttcagccac ggaccgggca catacatcgt
      721 ctgttggggg ccgatattcc aatagcggcc gagattgtgg ccgttgaccc agacgaaacc
      781 tttgccccac ggacgcatgt cgaggaagca atcgccgggc tgatcgattt cgacggtggc
      841 gcgccagaag gcaggcgcat ccacggcggc ggtgtctgcg ttttctgcgg cgtcgaaacg
      901 caacgcgtcg agcatcgcgg catcgagtgg caggggaaaa atctgccagt cacgcagttc
      961 aaccacggtt tcgccggaaa cgacgaatgt aaccggaccg tggatacctt tgcggtcgtg
     1021 cacttcgacg ccgaagttca ctcggcccat cgcttcaacc agaatgtcga gcgtggcggg
     1081 ctgcgatcgc tccggcagtt gcactcggaa attacgtgag cggcgatcgg tgaagccgat
     1141 tcgctcgccg ttcagaaaga cttgtccgat gtcgtgaatc gcggtggcct cgagcgttcc
     1201 ggcgggtccc gccgggagtt gcgttcgata cagaatgcag ccgaaggcct ggtcgtagag
     1261 ttccatcgtg cgcggatgct catcggtgag cggttccggt agattggaaa acacgcgagc
     1321 cacttgcgtg gccggaaccg gtgagatcgc gatgacggga ttccgcgcag gtggctctgg
     1381 aagcgtttct ccgggtagca ggtgcttcgc gagcaacgcg cgtgtttgct cgaatttggg
     1441 tgtcacccaa cccgcttcgc tgacgggcgc gtcgtagtca tagctcgatg tgtccggttt
     1501 gaacggccga tcggcgccgg tgaagaagcc gaacgtcgtc ccgccatgcg ccatgtagat
     1561 gctgaacgag gcgcggacct cgagcatgta gccgaggtct gccaggtagc gctcggtgtt
     1621 gcccgtgtga tgcggcgcgc cccaggtgtc gaaccagccg ggatagaatt caccgcacat
     1681 gagcgggccg gtcggcagga tttcgcgcag cgctttaaag ccgccggcgg gatcggagcc
     1741 gaagttgacg acgggaaaca gatcgtcgcg atagccgtca cggaggtgct gcttcggatt
     1801 gcaggcgaag agcggcacat cgaagccgcc atcgagcagc gcttgtcgga gttcgcccat
     1861 gtaggcggcg tctttgccgt agaagccgta ttcgttttcg acctgaacca tcaggatcgg
     1921 gccgccgtgc gtaacttgca gcggagccaa cacgcggccg acttcgatca ggtagcgttt
     1981 cgctgcttcc aaatagcgtg ggtcgcggga gcggagcttg atgtcgtcgt gtttcagcag
     2041 ccaccacggc aagccgccca tttcccattc cgcgcacgcg taagggcccg gccgcagaat
     2101 gacccaaagg ccttctgcct gtgcggcgcg gcaaaactcc acgacgtcgg cctggcccga
     2161 ccagtcgtat tcgccgggac ggggctcgtg catgttccag aaaaggtaag cgcagacggt
     2221 gttgagcccc atggctttta tcatgcgcag ccggtgctgc cagtattcgc gcggcacgcg
     2281 gggtgcgtgc agttcgccgc agcggatctg aaagcgattt ccatcgagca gaaaatcgtc
     2341 gtcgccgatc tcgaagcggt gggtcgctgc ggtttcgtgc
//
LOCUS       DASPLT010001626         1079 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_143335, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001626 DASPLT010000000
VERSION     DASPLT010001626.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1079)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1079)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1079
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_143335"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..476
                     /locus_tag="VEA63_16545"
     CDS             <1..476
                     /locus_tag="VEA63_16545"
                     /inference="COORDINATES: protein motif:HMM:NF026255.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HYD85680.1"
                     /translation="LRLGPKFSGFAAFNVFKMVTDGGSTSALGSDAVNWMGRINVTSE
                     LTKTTVLTTAYFYRAPMQIERGRFEAQHGANLALRKKLNGDKASVTLRVNDPFGTQRF
                     RVRAENDDVLQITERNFGARMVWLGFNYSYGRPPRVRQVQQDNSNQGSAFPPPSE"
     gene            744..>1079
                     /locus_tag="VEA63_16550"
     CDS             744..>1079
                     /locus_tag="VEA63_16550"
                     /inference="COORDINATES: protein motif:HMM:NF024436.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD85681.1"
                     /translation="MSMHSLSLRVLSAAMLAATLTACRTGTHVARMDVNQTADISGNW
                     NDTDAKLVADTMIKQALNTPWVTRYMQSHAGKTPTVIVGTITNRSSEHINTNVFINRL
                     ENAFVNSGAV"
BASE COUNT          207 a          369 c          314 g          189 t
ORIGIN      
        1 acctgcgact tgggccgaag ttcagcggct tcgcggcgtt caacgtcttc aagatggtga
       61 ccgacggcgg ctccacatcg gccctcggct cggacgccgt gaactggatg ggccgcatca
      121 acgtcacgtc ggagctgacg aagacgacgg tgctcaccac cgcttacttc taccgcgcgc
      181 cgatgcagat cgagcgtggc cggttcgagg cgcagcacgg cgcgaacctc gcccttcgca
      241 agaagctcaa cggcgacaag gccagcgtga ccctgcgcgt gaacgatccg ttcgggacgc
      301 agcgcttcag ggtccgcgcg gagaacgatg acgtcctcca gatcaccgag cggaatttcg
      361 gcgcccggat ggtgtggctc ggcttcaact acagctacgg ccgtccgccc cgcgtgcgtc
      421 aggtccagca ggacaattcg aaccagggct cggccttccc gccgccgtcc gagtaacgct
      481 gtaaatcaaa ggtttagaag ttccggcccc gccgacggtc tcaggactgc ggcggggctg
      541 gtcttttgtg cacggtggga taccgagcgc gaccgctcgc gtctgtgagc ctttcatata
      601 gaaggggaat cgggcatttg cccgttgcga tagcgtgcgc aatcccacaa agtagatatg
      661 acctgagacg cctggtgttt acccccccgc cgcgtccgcc agccatccca ccacaggttt
      721 tcaccctctc atacctgacg acgatgtcaa tgcattcgct ctccctccgc gtgctttccg
      781 ccgccatgct cgccgcgaca ctcacggcgt gccgcaccgg tacccacgtc gcgcgcatgg
      841 acgtgaacca gaccgcggac atctcgggca actggaacga caccgacgcg aagctcgtgg
      901 ccgacacgat gatcaagcag gcgttgaaca cgccctgggt cacgcgctac atgcagtcgc
      961 atgccggcaa gacgcccacc gtgatcgtcg gcacgatcac gaaccgctcg tccgagcaca
     1021 tcaacaccaa cgtgttcatc aatcgcctcg agaacgcttt cgtcaactcc ggtgctgtc
//
LOCUS       DASPLT010001627         1914 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_80414, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001627 DASPLT010000000
VERSION     DASPLT010001627.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1914)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1914)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1914
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_80414"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..310)
                     /locus_tag="VEA63_16555"
     CDS             complement(<1..310)
                     /locus_tag="VEA63_16555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HYD85682.1"
                     /translation="MSFEKILIVEDELVVRNLLQSIFQRHKLPVTTANNLADAAAILQ
                     REQFDLMMLDIRLPDGDGQKFLEQVAALPERPLVVMVTGYGTIESAVGCMRAGAFDYV
                     L"
     gene            complement(419..862)
                     /locus_tag="VEA63_16560"
     CDS             complement(419..862)
                     /locus_tag="VEA63_16560"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HYD85683.1"
                     /translation="MLSSSAALFPNLEPAAAPAAVGKRETVVLLVEDEDSLAELLTCL
                     FSRIKVRVMRAADGAQARRVFAENRADITLAFVDCHLPDGHGADLCAEFRTIVPNLPL
                     LLTSGRDQQALAVTLAAGGPCDFLPKPYMPADVMRRVRSLLGLAA"
     gene            978..1373
                     /locus_tag="VEA63_16565"
     CDS             978..1373
                     /locus_tag="VEA63_16565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD85684.1"
                     /translation="MRLLSFLTDIERAIRLEANAGEGEIWDSARIVNFKTGLARLTLS
                     AREHDSEMPQGTILVQHFALANGSFCLKASLNWQGSEASPVMSVYDTPLLNWKLEASR
                     IGSSWLAGPPAEATLAHASMEHERIAVAG"
     gene            complement(1574..>1914)
                     /locus_tag="VEA63_16570"
     CDS             complement(1574..>1914)
                     /locus_tag="VEA63_16570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD85685.1"
                     /translation="LGALDELVAQESATLNQRDFGAVADIQRRAQPLVDALSDLGIAA
                     ADDLARARVAGLLARRQHNIDFLESQLATARAELLSVQESTRRVACIAPAYGQPKRVS
                     GENSSFRAAG"
BASE COUNT          387 a          624 c          579 g          324 t
ORIGIN      
        1 tcaacacgta gtcgaacgcg cccgcgcgca tgcagccgac ggccgactcg atcgtgccgt
       61 agcccgtcac catgacgacg agcgggcgct cgggcaacgc ggcgacttgc tcgagaaatt
      121 tctggccgtc gccgtccggc agccggatgt cgagcatcat caagtcgaac tgctcgcgct
      181 gcaaaatcgc cgcggcgtcc gccaggttgt tcgccgtggt gacagggagc ttgtgccgtt
      241 gaaaaatgct ttggagcaga ttccggacga ccaactcgtc ctcgacgatc agtatcttct
      301 caaaagacat tacaggacaa acatgatggg aacggcgcgg ctgtcgcgta gaaacaacag
      361 aatggaccgc gaaataaaac gcccccgcga cgggtttcaa acaaatgttc gcgccgcgtc
      421 acgcagccag gccaagcagc gacctcaccc ggcgcataac atccgccggc atgtaaggtt
      481 tgggcaagaa gtcgcacggc ccgcccgccg cgagcgtcac ggcgagcgcc tgttgatcgc
      541 gtccgctcgt gagcagcaga ggcagattcg gcacaatcgt gcggaactcg gcgcacaaat
      601 ccgcgccgtg cccatcgggc aaatggcaat ccacgaaggc cagcgtgatg tcggcgcggt
      661 tttccgcgaa aacccgccgg gcctgagcgc cgtcggccgc gcgcatcacc cgcactttga
      721 tccggctaaa caagcacgtg agcagttccg ccagcgaatc ttcgtcctcg accagcaata
      781 cgacggtttc gcgcttcccg accgcggcgg gagcggctgc cggctccaag ttgggaaaaa
      841 gagcggccga cgaagacaac atcgtgcggt tctacggcgc ggcggcgtga aaactttaat
      901 aaaagcctca acttttttgc cgtcaggccg attaggtcga aacgtcgcac gtgtgcgtcg
      961 aaacccgcaa aaccatcatg cgtttactct ccttcctcac cgatatcgaa cgcgcgattc
     1021 gtctcgaggc caatgccggc gagggcgaaa tctgggactc cgcgcggatc gtgaacttta
     1081 aaaccggcct cgcgcgactg actttgtcgg cacgcgaaca cgacagcgag atgccgcaag
     1141 gcacgattct cgtgcaacac ttcgcgctgg cgaacggctc attctgcctc aaggcgagcc
     1201 tcaactggca gggttcggaa gccagcccgg tgatgtcggt ctacgatacg ccgttgctga
     1261 actggaagct ggaggccagc cgcatcggtt cctcatggct cgccggcccg ccggccgaag
     1321 cgaccctggc gcacgcctcg atggagcacg aacgcatcgc ggtcgcgggt taacaaacgc
     1381 cgattcgctc cagaaaaatt tcacccgctc ctttcgagcg ggttttttat tggcggtcgt
     1441 cgccgagagc ggcagccgtg gagcccgatg tcctcatccg gctgaacgac gttcgcctgc
     1501 aaaacaaccc gacgaggacg tcgggttcca ccttcaggat tgtttccgct cgcggctgag
     1561 ccggcgccgc agatcaaccg gccgcgcgaa agctcgaatt ttcgccgctg actcgcttcg
     1621 gctgcccata ggcaggcgcg atgcaggcga cgcgtcgcgt gctctcctgc acggagagca
     1681 gttccgcacg ggcggtcgcg agctgcgatt cgaggaaatc gatgttgtgc tgacgacgcg
     1741 ccagcaagcc tgccacgcgc gcacgcgcca aatcgtcggc cgccgcgatg ccgagatcgc
     1801 tcagcgcgtc caccagcggc tgggcgcgtc gctggatgtc cgcaaccgcg ccgaaatccc
     1861 gctggttcaa cgtcgctgat tcctgcgcca cgagttcgtc gagcgcgccg agca
//
LOCUS       DASPLT010001628         1166 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_285126, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001628 DASPLT010000000
VERSION     DASPLT010001628.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1166)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1166)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1166
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_285126"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..234
                     /locus_tag="VEA63_16575"
     CDS             <1..234
                     /locus_tag="VEA63_16575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009507961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar GTP-binding protein"
                     /protein_id="HYD85686.1"
                     /translation="AAYDRASMRDAYTAGRDLGATHLVFTHLDEVPQWGKLWDFLFDE
                     ELEPLFLATGPSLTGDCEEDVSGAIARRTLPEN"
     gene            249..488
                     /locus_tag="VEA63_16580"
     CDS             249..488
                     /locus_tag="VEA63_16580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD85687.1"
                     /translation="MKFVFLAGGLVGFLAAAVTGYLADRSADRIFFDAAVGCLVGALL
                     FRWFWTILVRGIRETILIRHAASTASAAANTPKHK"
     gene            509..>1166
                     /locus_tag="VEA63_16585"
     CDS             509..>1166
                     /locus_tag="VEA63_16585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HYD85688.1"
                     /translation="MNSPATVAPGMDAAPKTVWRAYQGVSPSAVDEKDLIERHLYLVR
                     NVVDRIKLNLPTHIDADDLYSVGVTGLMAAVRKFDPQQGCTFASYAAMRIRGSILDEL
                     RRMDWCPRRARARSRKLKETIIEVEQKLGRAATDEEVCSALGLSQKDYAKWVEDARPV
                     TFLAIDHRNEGEEGEGASLHELLKDETDVTGRENLEKQELLELLTQRMAELPDIPKKI
                     L"
BASE COUNT          212 a          387 c          337 g          230 t
ORIGIN      
        1 gccgcctacg atcgcgcgtc gatgcgcgac gcctacaccg cgggccgcga cctcggcgca
       61 acgcatctcg tgttcacgca tctcgatgaa gtgccgcagt ggggcaagct ctgggacttc
      121 ctcttcgacg aggaactcga gccgctgttt ctcgccaccg gtccgtcgct caccggggat
      181 tgcgaagaag atgtctcggg cgcgatcgcc cgccgcacgc tccccgaaaa ctgatccctt
      241 ttttgctcat gaaattcgtt tttctcgccg gcggtctcgt cggatttctc gccgccgcgg
      301 tcactggcta tctcgccgat cgttctgcgg accggatttt tttcgatgcc gcggtcggct
      361 gcctcgtcgg ggcgctgctg ttccgctggt tctggaccat tctcgttcgc ggtatccgcg
      421 aaaccattct catccgtcac gctgcgtcca ctgcttcggc cgcggcgaac acacccaagc
      481 acaagtaatc gtttccaatt aggatatcat gaactccccc gccaccgttg caccaggcat
      541 ggatgccgct cccaaaaccg tgtggcgcgc ctatcaaggc gtgtcaccca gtgccgtcga
      601 tgagaaggat ctcatcgagc gtcacctcta cctggtgcga aacgtggtcg atcgcatcaa
      661 actcaacctg cccacgcaca tcgacgcgga cgatctctac agcgtcggcg tcaccggcct
      721 catggccgcc gtgcgcaagt tcgatccgca gcagggctgc accttcgcga gctacgccgc
      781 gatgcgcatc cgcggctcga tcctcgacga gctccgccgc atggactggt gtccgcgccg
      841 cgcccgcgcg cgttcgcgca agctcaagga gaccatcatc gaggtggagc aaaagctcgg
      901 ccgcgccgcg accgatgagg aagtctgttc tgcactcggt ctttcccaga aggattacgc
      961 gaagtgggtc gaggacgcgc gtcccgtgac gtttctcgcg atcgaccacc ggaacgaggg
     1021 tgaggaaggg gagggtgctt cgctccacga attgctgaag gacgagaccg atgtgaccgg
     1081 tcgcgaaaac ttggagaaac aggagctcct cgagcttctc actcaacgca tggccgaatt
     1141 gcccgatata ccgaagaaga tcctcg
//
LOCUS       DASPLT010001629         1332 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_163425, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001629 DASPLT010000000
VERSION     DASPLT010001629.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1332)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1332)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1332
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_163425"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..920
                     /locus_tag="VEA63_16590"
     CDS             <1..920
                     /locus_tag="VEA63_16590"
                     /inference="COORDINATES: protein motif:HMM:NF045201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1343 domain-containing protein"
                     /protein_id="HYD85689.1"
                     /translation="ATSAQLEAIDALVIDLQDIGTRSYTFVGAMKSAMEYCFENGKEL
                     VVLDRPNPLGGLKVGGPMLDAEWMTDVGRFRVPYVHGLTIGELAHMARNFRPPGGIAV
                     SGEARARGKLTVVPMRGWSRSMRWPETGLAFVPTSTRIRDFAAVLGYPMTGLGCIIGD
                     FSHGVGGLHDFRGINHPRIPPEALEKELRSLKIPGVQFRRFSVPHAKGGKPASGVYVE
                     INDFDEWQPCDLNFWLMTLACRTSPGNPFTLLSAAKRREFLIHVGSEAFYNEIATMGA
                     DTDIEAWLRTWREQARIYQEQSKRYWLYR"
     gene            1116..>1332
                     /locus_tag="VEA63_16595"
     CDS             1116..>1332
                     /locus_tag="VEA63_16595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD85690.1"
                     /translation="MTPKPLRNNPSAEEKPDGSAWRARWHEVIFEADTPAGKRFDLAL
                     LGLILSSVLAVCLESVRELREAHGPTLR"
BASE COUNT          246 a          387 c          445 g          254 t
ORIGIN      
        1 gtgccacgtc cgcgcagctc gaggcgatcg atgcgcttgt gatcgatctc caagacatcg
       61 gcacgcgcag ctacacgttc gttggcgcga tgaaatcggc gatggaatac tgtttcgaga
      121 acggcaagga actcgtcgtg ctcgaccggc ccaacccgct gggaggcctg aaggtcggcg
      181 gcccgatgct cgacgctgaa tggatgactg atgtcggacg ctttcgtgtg ccgtatgtgc
      241 atgggctcac gatcggcgag ctcgcgcaca tggcgcgcaa cttccggccg ccgggcggca
      301 tcgcggtgtc cggtgaggcc cgagcgcgcg gcaagctgac cgtcgtgccc atgcgcggct
      361 ggtcgcgcag catgcgctgg cccgagaccg gcctcgcctt cgtgcccaca tcgacgcgca
      421 tccgcgattt cgcggcagtg ctcggttacc cgatgacggg cctcggctgc atcatcggcg
      481 acttcagcca cggcgtcggt gggctgcatg attttcgcgg catcaatcat ccgcgcatcc
      541 cgcccgaggc gctggaaaag gagctacgct cgctgaagat ccccggagtt cagtttcgtc
      601 gttttagcgt gccgcatgcg aagggcggca aaccggcgtc aggcgtttac gttgagatca
      661 acgattttga cgaatggcag ccatgcgatc tcaacttctg gttgatgacg ctcgcatgcc
      721 ggacgtcgcc aggaaaccca ttcaccctgc tgtcggccgc aaaacgccgc gaatttctga
      781 tccatgtggg gtcggaagcg ttttataacg aaatcgcgac catgggggct gacacagaca
      841 tcgaggcgtg gctgcgcacg tggcgagagc aggcgcggat ctatcaggag cagagcaagc
      901 gttactggct gtatcggtga cagacgaatg cggccgggcg atcgccataa agcgggcgga
      961 tttaacccgt tgtttgcagg atatccctac cttgaacccc gcgtgacgag gcgtttcgtg
     1021 ttccttgata cgtgacgatc gagaggcgtc gaggcagttg cccgcagcaa agggcgactg
     1081 gcgggacggg cggttccgcg ttctttgcgg aagacatgac tccgaagcct ctcaggaata
     1141 atccgtcggc agaagaaaag ccggatggta gcgcgtggcg ggcgcggtgg cacgaggtga
     1201 ttttcgaggc cgatacaccc gccggcaaac gcttcgatct cgcgctgttg gggctcatct
     1261 tgtcgagcgt gctcgcggtc tgtctggaga gtgtgcgcga gctgcgggaa gcgcatggac
     1321 caacactgcg cg
//
LOCUS       DASPLT010001630         2308 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_306855, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001630 DASPLT010000000
VERSION     DASPLT010001630.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 2308)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2308)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2308
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_306855"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2308
                     /locus_tag="VEA63_16600"
     CDS             <1..>2308
                     /locus_tag="VEA63_16600"
                     /inference="COORDINATES: protein motif:HMM:NF024603.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HYD85691.1"
                     /translation="GSVQSIDEFAGILYAGLANGVATATTPYTYLSKKSPLHGVAAFA
                     KDTAIAVHFPQLAPPTAMLDSHFELAVNDIAGAGFSPILADHRLTIAPAVPLAVGDSV
                     RLDFTSVNGPLIGDELRTTQVLAATVAPAVASEITVAAVAPHRLPLGPTPVPITISGT
                     ALNTVTAVRVGGITVAIDQQHAETLTFSVTPSNLAAFANVELVSSVSGAEVVTAAGFI
                     AVVPPLAVTHVTPAVTGYLAGVTATIEGSGFARDVRLYVNDVLTPIENVSGDSQLTFV
                     IPAGNQPLTLRLERSGAEPLVLANAIARTDNVPPRPMSIAPAAPAKDLPIEQTFRVTF
                     SESIDVAQFALAAPLYLTDAQDQPVPATYHWTQLNTVVDVVPTSFLGLGRRYTLRFQG
                     AIDTTGNASLPSENFAVNYLTVDQSPPTVAMSSPAAGATLLANSVVTLQAAVADNLQG
                     VAIFKDAQNNPLEEGVTAARVVFYYNGEVIGPATAIVGGVASRSFTIPEVGSIISRNF
                     HATAVDRSGNSATSAPVTVSVRPTIRPIIAFEEPTPADGSALAELSTFTIAANATVDL
                     PFARTELKINDALVRTSTSANATFAAIPVPAASVGTSFTVSVTAFDVQGVASLPTVRS
                     YTIAAESTPPAITLISPPANARIAAGQSLRIAASATDNKGIASVSFYLNGQASPAAVL
                     THAPYAFTLDSTAIPTGSAIAVRAVATDVTGLTAEDSRTITVVADDTKPVVAIVSPAA
                     GEIFGEGHQVRIVVQATDDQRVARVELSV"
BASE COUNT          396 a          739 c          708 g          465 t
ORIGIN      
        1 gcggcagcgt ccaatccatc gatgaattcg ccggcattct ctacgcaggg ttggccaacg
       61 gcgtcgcgac cgcgacgacg ccgtatacgt atctgtcgaa aaagtcgccg ctgcacggcg
      121 ttgctgcgtt cgccaaagat actgcgatcg ccgttcactt cccgcagtta gccccgccga
      181 cggcgatgct cgactcgcac ttcgaactcg ccgtcaacga catcgctggt gccggcttct
      241 ctccgattct cgcggatcac cgcctgacga ttgcgccggc agttccgctc gcggtcggcg
      301 attccgtgcg tctcgacttc acctctgtca acggcccgct gatcggcgat gaactacgca
      361 ccacccaagt tctcgcggcg acggttgcgc ccgcagtcgc aagtgaaatt acggtcgccg
      421 cggtcgcgcc gcatcgtctt cccttggggc cgacgccggt gccgattacc atttccggaa
      481 cggcactgaa cacggtgacc gcggttcgcg tgggtggcat tacagtcgcg atcgatcagc
      541 agcatgcgga aacactcacc ttcagcgtca caccgtcgaa tttggcggcg tttgccaatg
      601 tcgagttggt ttcttcggtg agtggtgcgg aagtcgtaac ggcggccggt ttcattgcgg
      661 tggtgccgcc gctcgctgtc actcacgtca ctccggccgt tacgggctat cttgccgggg
      721 tgaccgcgac gatcgagggg agcgggtttg cccgggacgt gcgcttgtat gtcaacgacg
      781 tcctcacgcc gatcgagaac gtgagcggcg attcacagct cacgtttgtg attccggcag
      841 gaaatcagcc gctgacgttg cgcctcgaac gcagcggcgc cgaaccgctg gtgctcgcga
      901 acgcaattgc gcgcaccgac aatgtcccgc cacggccgat gagcatcgcg cccgccgctc
      961 cggcgaagga tctgccgatc gagcaaacct tccgtgtcac gttttcagag tcgatcgatg
     1021 ttgcgcagtt cgctctggcg gcgccgctct atttgaccga cgcccaggat caaccggtgc
     1081 cggccaccta tcattggacg cagctcaaca cggtggtgga cgtcgttccg acttcgttcc
     1141 tcgggcttgg tcggcgctac acactgcgct tccaaggtgc gatcgacacc accggcaacg
     1201 cgtcgctgcc gtctgaaaat tttgcggtca attacctgac ggtcgaccaa tcgccaccga
     1261 cggtcgcgat gagttcaccc gctgccggcg ccacgttgct cgcgaactcg gtagtcactt
     1321 tgcaggcggc cgttgccgac aatctccagg gtgtggcgat cttcaaggat gcacagaaca
     1381 atcctctcga ggagggcgtc acggcggcgc gcgtcgtgtt ctattacaac ggtgaggtca
     1441 tcggaccggc gacggcaatt gtcggcggag tcgcgagccg ctcctttacg atcccagagg
     1501 ttggatcgat catctcgcgg aatttccatg cgaccgccgt ggaccgttcg ggaaactccg
     1561 cgacgtcggc gccggtgacg gtttcggttc ggccgacgat tcgcccgatc atcgcgttcg
     1621 aagagccgac tcccgcggat ggttcagcgc tggcggaatt gagcacgttt accattgcgg
     1681 cgaatgcgac cgtcgatctg ccgtttgcgc gcacggagtt gaagatcaac gatgccttgg
     1741 ttcggacgtc gacgagcgcg aatgccacct ttgctgccat tccggttccg gcggcgtcgg
     1801 ttggcacgag cttcaccgtg tcggtgaccg cgttcgacgt gcaaggcgtg gcgagtctac
     1861 cgactgttcg cagctatacg atcgccgcgg aatccacacc gccagcgatt acgctgatca
     1921 gcccgccggc taatgcccgg atcgccgccg gccaatcgct gcgcatcgcc gcgagcgcga
     1981 cggacaacaa gggcatcgca tcggtctcgt tctatcttaa cgggcaggcc agcccagctg
     2041 cggttctgac gcacgcgccg tatgcattta cactcgattc gacggcgatt ccgacgggaa
     2101 gtgccattgc tgtcagagcg gtcgccaccg atgtgacggg cctcaccgcg gaagattcac
     2161 gcacgattac ggtcgtcgct gacgacacga agccggtcgt ggcaattgtt tctcctgctg
     2221 caggtgagat cttcggcgag ggccaccaag tgcggatcgt cgttcaagcg accgacgatc
     2281 agcgcgtggc gcgtgtggag ctctccgt
//
LOCUS       DASPLT010001631         1337 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_80518, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001631 DASPLT010000000
VERSION     DASPLT010001631.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1337)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1337)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1337
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_80518"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..230)
                     /locus_tag="VEA63_16605"
     CDS             complement(<1..230)
                     /locus_tag="VEA63_16605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD85692.1"
                     /translation="MMRYFDRLRRCFFLVGLLLAATAVAAERAPLREYDVLRSFPPGR
                     LAALHAGDKPDANGFTGGNRAKGFWIEVGPQRG"
     gene            complement(227..1084)
                     /locus_tag="VEA63_16610"
     CDS             complement(227..1084)
                     /locus_tag="VEA63_16610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD85693.1"
                     /translation="MARYYYSYRTLARLDGDGRFAAMTAQIERNAEPQLTGELLTFLA
                     EPEFNEDLPAEQPLPTDYAKVFAYSHLARIRRGSISGTVLAQNSTLFSFRKGAAALEA
                     VRFASAFFGKGQFVGERLEVRDGSYHLEQKLTGPYFQPLSADQIAHGEQVRMAPNGTL
                     AVDGRAARAQSNIQQLVSRITVSEDRGRFRLIFDISGTEGVPVALELAFRRGGRFEGV
                     EPVDDVADAFLLKSGKGRYTAGADTITFGTGHADHTWTQLRGALPKWDGQCVYLTGST
                     PFQLNLEIS"
     gene            complement(1097..>1337)
                     /locus_tag="VEA63_16615"
     CDS             complement(1097..>1337)
                     /locus_tag="VEA63_16615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD85694.1"
                     /translation="LARIDQWLAETIDLDPDGQYTEKSTSVYSPVVDRALLTVARLLD
                     RPALREPVRRNLEMTLYYVHPDGEVVTEASKRQDK"
BASE COUNT          244 a          417 c          442 g          234 t
ORIGIN      
        1 ccgcgctgcg gaccgacttc gatccagaat cccttggcgc gattgccgcc ggtgaaaccg
       61 ttggcatcgg gtttgtcgcc tgcgtgcagc gccgcgagac gccccggcgg aaaggaccga
      121 agaacgtcgt attcgcgcag cggcgcgcgc tcggcggcga ccgcggtggc ggccagcagc
      181 aggccgacga ggaaaaaaca acggcggaga cgatcgaaat aacgcatcat gaaatttcca
      241 agttgagttg aaacggcgtc gagccggtca ggtaaacgca ctgcccgtcc catttcggga
      301 gcgcgccgcg cagttgagtc caggtatgat ctgcgtgccc cgtgccaaaa gtgatcgtgt
      361 ccgcaccggc cgtgtagcgg cctttgccgg acttcagcag gaaagcgtcg gcgacgtcgt
      421 caaccggttc gacaccctcg aatcggccgc cgcgccggaa cgccagttcc aacgccaccg
      481 gcacgccctc ggtgccggaa atgtcgaaga tcaggcggaa gcgtccgcgg tcctcggaga
      541 ccgtgatgcg cgaaacgagc tgctggatgt tgctctgcgc gcgggccgcg cgtccatcga
      601 cggcgagcgt gccgttcggc gccatgcgca cctgttcgcc gtgagcgatc tgatccgccg
      661 acagtggctg gaaatacggg ccggtcagct tttgctcgag atgataactg ccatcgcgaa
      721 cctccaggcg ttcgccgacg aattgtccct tgccgaagaa cgcgctggcg aaccgcacgg
      781 cttcgagcgc cgcggcgcct ttgcggaacg aaaacaaagt cgaattctgc gcgagcacgg
      841 tgccactgat cgatccacgc cggatgcgcg cgagatgcga atacgcgaag accttcgcgt
      901 agtccgtcgg caagggttgt tcggccggca ggtcttcgtt gaattccggt tccgcgagaa
      961 acgtcagcag ttcgcccgtg agctgcggct cggcgttgcg ctcgatctgc gcggtcatcg
     1021 cggcaaaccg gccgtcgccg tccagccgcg ccagcgtgcg gtagctgtag taatagcgcg
     1081 ccatgctgcc gcgctgttat ttgtcctggc gtttcgaggc ctccgtcacg acctcgccgt
     1141 cgggatggac gtagtagagc gtcatctcga gattgcgccg caccggttcg cgcagcgcag
     1201 gccggtcgag cagtcgggca acagtgagca acgcgcggtc gacgacgggc gaataaaccg
     1261 aggtgctttt ttccgtatac tgtccgtccg gatcgaggtc gatggtttcg gccagccatt
     1321 gatcgatgcg agcgaga
//
LOCUS       DASPLT010001632         1070 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_285224, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001632 DASPLT010000000
VERSION     DASPLT010001632.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1070)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1070)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1070
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_285224"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(35..>1070)
                     /locus_tag="VEA63_16620"
     CDS             complement(35..>1070)
                     /locus_tag="VEA63_16620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012377005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HYD85695.1"
                     /translation="IRDVFAPPSPPLPWKPEPAAPETITFAQSINHALRKILAERSES
                     IVLGQDIGTYGGAFKVTENLLNEFGRSRVFNTPLAESACTGYAIGMAVNGHRPIEEFQ
                     FSDFATEAMTQITLNAATFHYRSGAACPLVFRFPCGGNLTLGSFHSQELESVLLAMPG
                     IKALYPSTPQDAFDALLAAYEDNNPVIFFEHKALYRRGKHPVSWNSNYRDVWNPKQLR
                     TGDYATVVSYGEMVHLATEVCDYVGAEYEHTFDLFDLRALSPLRLDAIHASVARTGRL
                     IVIHEARRTHGFGAELVARFTEQNFAALKAAPLRIGSLDIPVPFAPELEQAFRPTLDK
                     VIEQVTAWLE"
BASE COUNT          200 a          254 c          422 g          194 t
ORIGIN      
        1 ggagaacgag aacgattaag agaacgagaa tgatttactc gagccaggcg gtcacttgtt
       61 cgatgacttt gtcgagcgtg gggcggaagg cttgttcgag ttcgggcgcg aaggggacgg
      121 ggatgtcgag cgagccgatg cggagcggcg cggctttgag cgcggcgaaa ttttgctcgg
      181 tgaaacgcgc gacgagctcg gcgccgaagc cgtgcgtgcg gcgggcttcg tgaatcacga
      241 tgaggcggcc ggtgcgggcg acggacgcgt ggatcgcatc gaggcgcagc ggtgagaggg
      301 cgcgaagatc gaagagatcg aacgtgtgtt cgtattcggc gccgacgtag tcgcagactt
      361 cggtggcgag gtgaaccatc tcgccgtagc tgacgacggt cgcgtaatcg ccggtgcgaa
      421 gttgtttcgg gttccagacg tcgcggtagt tggaattcca cgacacgggg tgtttgccgc
      481 ggcggtagag cgccttgtgt tcgaagaaga tgaccgggtt gttgtcctcg taagcggcga
      541 ggagcgcgtc gaaggcgtcc tggggcgtgc tcgggtagag cgctttgatg ccgggcatgg
      601 cgaggagaac ggactcgagc tcctgggaat ggaaggaacc gagtgtgaga ttaccgccgc
      661 aggggaaacg aaacacgagc gggcaggcgg cgccggagcg gtagtgaaac gtcgcggcgt
      721 tgagcgtgat ctgcgtcatc gcctcggtgg cgaaatcgga aaattgaaat tcctcgatcg
      781 ggcgatgacc gttgacggcc atgccgatgg cgtagccggt gcaggcgctt tcggcgagcg
      841 gagtgttgaa cacgcgcgag cggccgaact cgttgaggag attttcggtg accttgaacg
      901 cgccgccgta agtgccgatg tcctggccga gcacgatgga ctcggagcgt tcggcgagga
      961 ttttgcggag ggcgtgattg atcgattgcg cgaacgtgat ggtctcgggc gccgccggtt
     1021 cgggcttcca cggaagcggc ggcgatggcg gggcgaagac gtcgcggatg
//
LOCUS       DASPLT010001633         1404 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_59363, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001633 DASPLT010000000
VERSION     DASPLT010001633.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1404)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1404)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1404
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_59363"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1233)
                     /locus_tag="VEA63_16625"
     CDS             complement(<1..1233)
                     /locus_tag="VEA63_16625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HYD85696.1"
                     /translation="MKPSSAPFYISRRSFVKRCSVLVAATGLPLWFVERQLLAAETPA
                     RPLSPNNRPRIALVGCGGMGKSDAGNASNHGDIVALCDVDDTHTAEAVARFTVDGKAP
                     ASFNDFRRVLERGDVDVVINATPDHWHTLINIACAKAGKDVYAEKPLTLTIDEGRHVV
                     KAVRDARIVLQTGTQQRSSKRFRLACELVRNGRIGKLKKADVWLPAGLVGGPFATAPV
                     PQGLNWDYWLGQSPEVEYTPERCHGTFRFWYDYSGGTMTDWGAHHNDIAYWAIGLIAP
                     TQVASTRLSQPVAGGYDAYADYVVNYTYENGIALNIHTTKDDSIYGEILNREGQRNGI
                     RFEGSDGWIWVNRQSINASDPALLEQPLPEDAQRLYVSNDHWQNFFDCVRSRELPICD
                     VETGHRSATMCHLGAISLR"
BASE COUNT          237 a          350 c          555 g          262 t
ORIGIN      
        1 gcggagggag atcgcgccga ggtggcacat cgtcgcggag cggtggccgg tctcgacgtc
       61 gcagatcggg agttcgcggg agcggacgca gtcgaagaag ttttgccagt ggtcgttgct
      121 gacgtagagg cgttgcgcgt cctcggggag cggctgttcg aggagcgcgg gatcgctggc
      181 gttgatgctc tgacggttga cccagatcca gccgtcgctg ccttcgaagc ggatgccgtt
      241 gcgctggcct tcgcggttga ggatttcgcc gtagatgctg tcgtccttcg tggtgtggat
      301 gttgagcgcg atgccgtttt cgtaggtgta gttcacgacg tagtcggcgt aggcgtcgta
      361 gccgccggcg actggctggc tgaggcgggt ggaggcgact tgggtgggcg cgatgaggcc
      421 gatggcccaa taagcgatgt cgttgtgatg cgcgccccag tcggtcatcg tgccgccgga
      481 gtaatcatac caaaaacgga aggtgccgtg gcagcgctcg ggcgtgtatt caacttctgg
      541 cgactggccg agccagtaat cccagttgag accctgcggc acgggcgcgg tggcgaacgg
      601 cccgccgacg aggccggcgg ggagccagac gtcggctttc ttgagtttgc cgatgcggcc
      661 gttgcggacg agttcgcacg cgaggcggaa gcgctttgaa ctgcgctgtt gcgtgccggt
      721 ttggagaacg atgcgagcgt cacggacggc tttcacgacg tggcggcctt cgtcgatcgt
      781 gagcgtgagc ggcttctcgg cgtagacgtc cttgccggct ttggcgcagg cgatgttgat
      841 caacgtatgc cagtggtcgg gcgtggcgtt gatgacgacg tcgacgtcgc cgcgctcgag
      901 aacgcggcgg aaatcgttga aggaggcggg ggctttgcca tcgacggtga agcgggcgac
      961 cgcctcggcg gtgtgcgtgt cgtcgacatc gcagagggcg acgatgtcac cgtgattgga
     1021 ggcgttgccg gcgtcggatt tgcccatgcc gccgcaaccc acgagggcga tgcgcggacg
     1081 gttattgggc gaaagggggc gggcgggcgt ctcggcggcg aggagctggc gttcgacgaa
     1141 ccagagcggg agaccggtcg cggcgacgag aacgctgcaa cgtttcacga aagagcggcg
     1201 cgagatgtag aatggggcgg aagacggttt catggggtga gagattgcaa gggttgacgg
     1261 aacgaagctg gaaaagattt aacgatcaag cgacgggatg gaaagggtca agacgaggct
     1321 ctgagtgaga cggcttacct ggagtaggtc ttggccaagg cggcggcggt gtgacgcaga
     1381 ctcgtgcgaa gttcggtcgg ggcg
//
LOCUS       DASPLT010001634         1016 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_204600, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001634 DASPLT010000000
VERSION     DASPLT010001634.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1016)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1016)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1016
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_204600"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..421)
                     /locus_tag="VEA63_16630"
     CDS             complement(<1..421)
                     /locus_tag="VEA63_16630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD85697.1"
                     /translation="MKPSKTNYRGAEALVFATRALQLVVTTSVGPRIVSLRSSAGKKR
                     NLLLEMPADEQRSHGYLFRGGHRLWHSPEDIVRTYQPDDDALALKSLPKGVALTQPVE
                     PKTGMVKAMAIELLGERTVKVTHTLTNRGLWTVECAPW"
     gene            complement(402..>1016)
                     /locus_tag="VEA63_16635"
     CDS             complement(402..>1016)
                     /locus_tag="VEA63_16635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HYD85698.1"
                     /translation="VIVVNKWDLVTKAFKEQGGFKPYKNERDYREKYEHALFERLYFT
                     PGSPVIFVSAVSGYEVDRMLNAAVKLNRMLDTKLSTAKLNKVIGYLAERTPPPAVGGK
                     RFRVYYATQTGTRPFRIKIFCNREEKLTEQYRRYLEAGLVEEFDLAGCPIYFDLVGKE
                     KHDPGMSFSAIRSAQEKAKSKPHTPKWRASEASTNDSPHETLED"
BASE COUNT          184 a          340 c          277 g          215 t
ORIGIN      
        1 cccacggcgc acactcgacc gtccacagcc cgcggttcgt caacgtgtgc gtcactttca
       61 ccgtgcgctc gccgagcagc tcgatcgcca tcgccttcac catgccggtc ttcggctcga
      121 cgggctgcgt caacgccacg cctttcggca gcgatttcaa cgcgagcgcg tcgtcgtccg
      181 gctgatacgt gcgcacgatg tcctcgggcg aatgccacaa acgatgcccg ccgcgaaata
      241 agtaaccgtg actccgctgc tcgtccgccg gcatctcgag cagcaggttc cgcttctttc
      301 ccgcagatga acgcagcgat acgatgcgcg gcccgaccga tgtcgtgacc accaactgca
      361 gcgcgcgcgt ggcgaacacc agggcttccg caccgcgata attagtcttc gagggtttca
      421 tggggtgaat cgttcgtgct ggcttcgctc gcccgccact tcggcgtatg cggtttgctt
      481 ttcgccttct cctgcgccga gcgaatcgcg gagaaggaca tgccggggtc gtgcttttcc
      541 ttcccgacca gatcgaaata aatcggacag cccgcgagat cgaactcctc gaccaaaccc
      601 gcttcgaggt agcggcgata ttgctccgtc agcttctcct cgcgattgca gaaaatttta
      661 atgcggaacg gccgcgtgcc cgtctgcgtc gcataataaa cgcggaaacg tttcccgccc
      721 accgccggtg gcggcgtgcg ctccgcgagg tagccgatga ccttgttcag tttcgccgtc
      781 gacagcttcg tatcgagcat gcggttcaat ttcaccgcgg cattcaacat ccggtcaact
      841 tcgtagccgc tcaccgccga cacgaaaatc accggcgaac ccggcgtgaa atacagccgc
      901 tcgaacagcg cgtgttcgta tttttcgcgg tagtcgcgct cgtttttgta aggcttgaat
      961 ccgccctgct ccttgaacgc cttcgtcacg agatcccact tgttgacgac gatgac
//
LOCUS       DASPLT010001635         1146 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_80570, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001635 DASPLT010000000
VERSION     DASPLT010001635.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1146)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1146)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1146
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_80570"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..236
                     /locus_tag="VEA63_16640"
     CDS             <1..236
                     /locus_tag="VEA63_16640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD85699.1"
                     /translation="RADLRSYRTARWKLVLDFLNPGRDELYDLGLDPAENVNLIDDPR
                     PEARVAHRELKKMIHDVMRANRDPIFVQSASAP"
     gene            233..>1146
                     /locus_tag="VEA63_16645"
     CDS             233..>1146
                     /locus_tag="VEA63_16645"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HYD85700.1"
                     /translation="MKSPLPLFRCLIVAPVLGLAALADASVSPTNVLFIAADDLRTDL
                     GCYGAAQVKTPHLDALARRGRLFEGAYVQQTVCNPSRASIMTGLRPDTVRVWDLQTHF
                     RAARPDAVTLPQHFKNHGYTTISIGKLFHNQGKRVPPEGPFPDPISWSEPPVFANGAH
                     WQDWVMPDGGPPPRKQGAVQCLDVPDNAYFDGKIADAACAKLGELKSAGQPFFLAVGF
                     WKPHLPFNAPKKYWDLYDRAKLNPPDPARAPTGAPAIAGHNSDELRNYGGIPKEGPIP
                     PELAAELRHGYYAGISFLDAQVGRLIAEL"
BASE COUNT          201 a          387 c          348 g          210 t
ORIGIN      
        1 cccgcgccga cttgcgcagc tatcgcactg cacgctggaa gctggtgctc gattttctca
       61 atccgggccg cgacgagctc tacgatctgg ggctcgaccc agcggaaaac gtcaatctca
      121 tcgacgatcc ccgacccgaa gcgcgcgtcg cgcaccgtga attgaaaaaa atgatacacg
      181 atgtgatgcg ggccaaccgc gacccgattt tcgtccaatc tgcctccgcc ccatgaaatc
      241 accgctgccc ctgttccgct gcctcatcgt cgcgcccgtg cttgggctgg ctgcgctcgc
      301 cgacgcgtcc gtctccccga cgaacgttct gtttattgcc gcagacgacc tgcggaccga
      361 cctcggctgc tacggcgccg cgcaggtcaa gaccccgcac ctcgacgcgc tggcccgccg
      421 cggccggctc ttcgaaggtg cttacgtcca gcagacggtc tgcaatcctt cgcgcgcgtc
      481 gattatgacc ggtctgcggc ccgatacggt ccgggtgtgg gatctgcaga cgcatttccg
      541 cgccgcccgg ccggacgccg tgacgctgcc gcagcatttc aagaaccatg gctacacgac
      601 gattagcatc gggaagctgt ttcacaacca aggcaaacgg gtgccaccgg agggtccttt
      661 tcccgatccg atctcgtggt cggagccgcc ggtttttgcg aatggagcgc attggcagga
      721 ttgggtgatg cccgacggcg gaccgccgcc gcgcaaacaa ggggcggtgc aatgcctcga
      781 tgtgcccgac aacgcttatt tcgacggcaa gattgccgac gcggcctgcg cgaagctcgg
      841 cgagctgaaa tcggccggac agccgttttt tctcgcggtg ggtttttgga agccgcacct
      901 gccgttcaac gcaccgaaaa aatattggga cctctacgac cgcgcgaagc tcaacccgcc
      961 cgatccggcg cgcgcgccca ccggcgctcc ggcgatcgcg ggccacaatt cggacgagct
     1021 gcgcaactac ggcggcattc cgaaggaggg cccgattccg ccggagctcg ccgctgagct
     1081 gcgtcatggc tattatgcgg gtatcagttt tctggatgca caggtgggcc ggctgatcgc
     1141 cgagct
//
LOCUS       DASPLT010001636         1759 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_20215, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001636 DASPLT010000000
VERSION     DASPLT010001636.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1759)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1759)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1759
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_20215"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1026
                     /locus_tag="VEA63_16650"
     CDS             <1..1026
                     /locus_tag="VEA63_16650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-D-arabino-heptulosonate 7-phosphate
                     synthase"
                     /protein_id="HYD85701.1"
                     /translation="PKSNRLTPEQVAEVTAVVTEFGCKVQPIIGAVRTIYAIVGDERD
                     ELMINRLEGLEFVERIDRIQSPFKLMDKRSDLATHRIAIGGVTLGEELLVIAGACTID
                     PKNPNYFYETAAAVKEAGAQVLRGGVWKPRTNPYTFQGDVKSLDILLEASRRTGLPVD
                     TEVMEEAHIKLALDAGVGALQVGARNALNYALLRQIGRAVAERPTTAVLLKRSIHMGP
                     LNEFISAAEYIAAFGNPNVILCPRGTTPTLDGYRNHPDESITPLLKEKTWAPVVVDPS
                     HSVGKASYVPAAALAAVAYGADGLCIEAHVEPAKGIGDDPKQALTPEMLKKTIAQAKE
                     LWALRRR"
     gene            complement(1175..>1759)
                     /locus_tag="VEA63_16655"
     CDS             complement(1175..>1759)
                     /locus_tag="VEA63_16655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HYD85702.1"
                     /translation="INRRFMFMGRWLKHAYYPNWNLRLFKHRLGRYERLVIGATQSGD
                     NEVHEHILVQGTTGRLRCEMDHYAFASIDVFVEKHNRYSNWEARLELEEGLEQTRFAT
                     PQAAPVRLRRTLKRIVRRMPFRPLLRFLYVYIFQAGFLDGREGYIFARLHGVYEFLAL
                     AKAAELRAQRTVQTNAALGERTAADSAELAPKAG"
BASE COUNT          355 a          477 c          609 g          318 t
ORIGIN      
        1 cccaaatcca atcgcctcac gccggagcaa gtggccgaag tcaccgccgt cgtgacggag
       61 ttcggctgca aggttcagcc gatcatcggc gccgtgcgaa cgatctatgc gatcgtgggc
      121 gacgagcgcg atgagttgat gatcaaccgg ctcgagggtt tggagttcgt cgagcgaatc
      181 gaccggattc agtcgccatt caagctgatg gacaaacgct ccgacctcgc gacgcatcgc
      241 atcgcgatcg gcggcgtcac gctcggtgag gaactgctgg tgatcgcggg cgcgtgcacg
      301 atcgacccga agaatccgaa ttatttctac gaaacggcgg cggcagtgaa ggaggcgggc
      361 gcgcaggtgt tgcgcggcgg cgtgtggaaa ccgcgcacga atccttacac gtttcaaggt
      421 gacgtgaagt cgctcgacat cttgctcgag gcgtcgcggc gcaccggttt gccggtcgac
      481 accgaggtga tggaagaggc gcacatcaag ctcgcgctcg acgcgggcgt cggcgcgtta
      541 caggtcggcg cgcggaacgc actcaactac gcgctactgc ggcaaatcgg ccgcgcggtg
      601 gcggagcgtc cgacgacggc ggtgctgttg aagcgaagca tccacatggg gccgttgaac
      661 gaattcattt cggcggcgga atacatcgcg gcgttcggaa atccaaacgt gattctttgt
      721 ccccgcggca cgacgccaac gctcgacggt tatcggaatc accccgacga aagcatcacg
      781 ccgttgttga aagagaagac gtgggcgccg gtggtggtag atccgtcgca ctcggtcggg
      841 aaggcgtcgt atgtgccggc ggcggcgctc gccgcggtcg cgtatggcgc ggacggattg
      901 tgcatcgaag cgcacgtcga accggcgaag ggcatcggcg acgatccgaa gcaggcgctg
      961 acgcccgaga tgctcaagaa aacgatcgcg caagcgaagg agctttgggc gttgcggcgg
     1021 aggtgaagat cgtggcgcgc tgccggccac atctttctct ttcttcttta tctttatctt
     1081 tcgtcaggga gcgcgcgacg cgtggatggg agagagaaag agaaagagaa agaagaaaga
     1141 gaaagatttt ggagggcgta gagcttgtga ctggctagcc ggctttgggt gcgagttcgg
     1201 cggaatccgc ggcggtgcgt tcgcccagcg cagcattcgt ttgcacggtg cgttgcgcgc
     1261 ggagttcggc ggctttggcg agcgcgagaa attcgtagac gccgtgcaac cgggcaaaga
     1321 tgtagccttc gcgaccgtcg aggaaaccgg cttggaaaat gtagacgtag agaaaacgca
     1381 ggagcgggcg aaagggcatc cgccggacga tgcgcttgag ggtgcggcgc aggcgcaccg
     1441 gcgcggcttg cggcgtggcg aaacgcgttt gctccagacc ctcctcgagc tcgagtcgcg
     1501 cttcccagtt cgagtagcga ttgtgctttt cgacgaagac atcgatcgac gcgaacgcgt
     1561 aatgatccat ctcgcaacgc agccgacccg tggtgccttg gacgagaatg tgttcgtgca
     1621 cttcgttgtc gccgctctgg gtggcgccaa tgacaaggcg ctcgtagcgg ccgaggcggt
     1681 gtttgaaaag gcgcaggttc caatttggat aatacgcgtg cttcagccag cggcccatga
     1741 acatgaagcg gcggttgat
//
LOCUS       DASPLT010001637         1133 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_163604, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001637 DASPLT010000000
VERSION     DASPLT010001637.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1133)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1133)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1133
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_163604"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..545)
                     /locus_tag="VEA63_16660"
     CDS             complement(<1..545)
                     /locus_tag="VEA63_16660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD85703.1"
                     /translation="MKMKHTALVKIWLIAAALPVAIWAQAPDPAPPLRDTTTSADPYM
                     RMPGDREFSIGGSGASSKDFDNTLGGVAFSYGSYINNTLAVVLRQSVNYSNPEVGGTQ
                     WNGSTRIALDQHVLARGRVRPFVGVNFGGIYGDQVNDSWAAGLEAGAKVYVQERTFIA
                     ATVDYSWLFESSSGLDDRFDDG"
     gene            860..>1133
                     /locus_tag="VEA63_16665"
     CDS             860..>1133
                     /locus_tag="VEA63_16665"
                     /inference="COORDINATES: protein motif:HMM:NF017935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF983 domain-containing protein"
                     /protein_id="HYD85704.1"
                     /translation="MHVTRLQVIRRGLSHRCPNCGEPTLFPPKSLQIHRRCPVCGVGL
                     DRGDGFFLGPWVLNYTVVVFCLVPIVVLSVRGIIPWVASLVLAGIGC"
BASE COUNT          199 a          389 c          308 g          237 t
ORIGIN      
        1 ccgtcgtcga aacgatcgtc caagccgcta gagctctcga acagccagct gtagtcgacc
       61 gtcgccgcga tgaacgtgcg ctcttgcacg taaactttag cgcccgcttc caatcccgcc
      121 gcccatgaat cgttcacctg gtcaccgtaa atgccgccga aattcacgcc gacaaacgga
      181 cggacccgcc cgcgtgccaa cacgtgttga tcgagcgcaa tgcgagtgga gccgttccac
      241 tgcgtgccgc ccacctcggg attgctgtag tttaccgact ggcgcagcac gacggcgagg
      301 gtgttgttga tataagaacc ataggaaaac gcgaccccac ccagcgtgtt gtcgaaatcc
      361 ttgctgctcg cgccgctgcc accgatcgaa aattcccggt cgcccggcat gcgcatataa
      421 ggatccgcgc tggtcgtggt gtcccgtagc ggtggcgccg ggtccggcgc ctgcgcccag
      481 atcgcgaccg gcaaagccgc ggcaatcagc cagattttca ctaacgcagt gtgtttcatt
      541 ttcatgatat gagttctttc ttgtttgtag tgcagatcgc tggtcccgcc gggaacgttt
      601 tcacccgctc tcatcgcgca acgacttcga cgcccggaaa ccctccaacc ccgagtgggg
      661 cgtgtcttct accgcggatc acccacccgg cgctggaaac cggcccccgc cgcacgtgtc
      721 gaaatcctcc tgccgaagaa acggatgccc acgccaccgc cgcatctcca aatttcggat
      781 acgccgatcc gctgcgcccc tcgtttgact ctgccatgga agcacccttg tatgcgccgg
      841 cccttcagcg gctatttgca tgcacgtcac tcgcctccaa gtcattcgcc gcggtctgtc
      901 gcaccgctgc ccgaactgcg gcgaaccgac gcttttcccg ccaaaatcgc ttcaaattca
      961 tcggcgctgc ccggtgtgcg gcgtcgggct cgatcgcggc gacggatttt ttctcggtcc
     1021 gtgggtgctc aactacaccg tcgtcgtctt ttgcttggtg ccgatcgtcg tgctcagcgt
     1081 gagaggaatc ataccctggg tcgcttccct cgtgctcgcc ggcatcggct gcg
//
LOCUS       DASPLT010001638         1207 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_306916, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001638 DASPLT010000000
VERSION     DASPLT010001638.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1207)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1207)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1207
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_306916"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(46..720)
                     /locus_tag="VEA63_16670"
     CDS             complement(46..720)
                     /locus_tag="VEA63_16670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HYD85705.1"
                     /translation="MSAIADDLISHLRSLRSEKAIAGMRRFGIAATGEQLGVSIATLR
                     ALARPHRKNHALALELWASGIHEARILATVIDDPRQITRGQMERWVRDCDNWAVTDSL
                     AFAFDRTDHAIAKAHAWSRRKPEFVKRAAFSMIAGLAVHRKELPDSVFVEFLPVIARE
                     ATDERNFVRKAVNWALRGIGKRNVRLRREAIAAAKRIQKIDSRGARWIAADALREFAK
                     SVPACR"
     gene            complement(764..>1207)
                     /locus_tag="VEA63_16675"
     CDS             complement(764..>1207)
                     /locus_tag="VEA63_16675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD85706.1"
                     /translation="AEDWELILRVWEVKKFRERKQFVARWTPATADAELAKLHEQVRL
                     FMEWSPEKNGLPYVPPASPRTWLETLGASLTLFLGEKNLLPKEQLAVSDQTLLRAAAA
                     ASTSEAASLAFITLRNRAEKLGVAQRLDAALAASPLIAQASNLSA"
BASE COUNT          220 a          425 c          359 g          203 t
ORIGIN      
        1 tcggagaggc gcccctacct gtgtcaccgc cgtgagattc ccgctttagc ggcaagccgg
       61 gacggatttg gcgaactcac gcaacgcgtc ggcggcgatc caacgcgcgc cgcgcgaatc
      121 gatcttctgg atacgtttcg ccgccgcgat ggcttcgcgc cgcagtcgga cgttacgctt
      181 gccaatcccg cgcagcgccc agttcaccgc cttgcgcaca aaattccgct catcggtcgc
      241 ttcgcgcgcg atcacgggca ggaactccac aaacacggaa tcgggcagct ccttccggtg
      301 caccgccaaa ccggcaatca tcgaaaaggc ggcacgtttc acgaactccg gcttccgccg
      361 gctccaggcg tgcgctttcg caatcgcgtg gtccgtgcgg tcgaacgcaa acgccagcga
      421 atcggtcacc gcccagttat cgcaatcgcg cacccaacgc tccatctgcc cacgcgtgat
      481 ctggcgcgga tcgtcgatca cggtggcgag gattcgcgct tcatgaatcc cgctcgccca
      541 caactccagc gcgagcgcgt gattcttgcg atgaggccgg gcaagcgcac gcagcgtcgc
      601 gatcgaaacg cccagctgct cgcccgtcgc cgcaatgccg aaacgccgca ttcccgcgat
      661 ggccttttcg ctccgcagcg agcggagatg cgaaatcaaa tcgtcggcga tcgcactcat
      721 gacgccggca gaaagacgaa atgctccgcg ccgcggcgca cgctcacgcc gacaaattcg
      781 acgcttgcgc gatcaacggc gaggccgcga gcgcggcgtc gagccgctgg gcgaccccga
      841 gtttttccgc ccggttgcgc agcgtgataa acgccagtga ggcggcttca ctcgtgctcg
      901 cggccgcggc ggcgcgaagg agcgtctggt ccgaaaccgc aagctgctct ttcggcagaa
      961 gatttttctc gccgaggaaa agcgtgagcg acgcgccaag cgtctccagc cacgtgcgcg
     1021 gcgaggctgg cggcacgtaa ggcagtccgt ttttttccgg gctccactcc atgaaaagcc
     1081 ggacctgctc gtgcagcttg gcgagttccg cgtccgcggt cgcgggcgtc caacgcgcca
     1141 cgaactgctt gcgctcacga aattttttca cctcccacac gcgcaggatc agctcccaat
     1201 cctccgc
//
LOCUS       DASPLT010001639         1043 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_204683, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001639 DASPLT010000000
VERSION     DASPLT010001639.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1043)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1043)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1043
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_204683"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1043
                     /locus_tag="VEA63_16680"
     CDS             <1..>1043
                     /locus_tag="VEA63_16680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit A"
                     /protein_id="HYD85707.1"
                     /translation="VGLGYLTLGQPLNTLSGGESQRLKLVRYLGGFAAEGSEFRILGS
                     GLKTQNSIPKTQNSEPCGALLLLDEPTTGLHRHDVKRLLSVLQALVDRGHSVVVIEHN
                     LDVLKSADWVIEIGPDAGANGGRVVAEGTPEDVARVVTATSPFLRAALAVSPGGSEFG
                     VSGLELAAAEPAAPYITSTSKLKTEKTETSGSELRIEGARENNLKNLSLTIPHRQLNV
                     VTGVSGSGKSTLAFDIVFAEGQRRFMESMSPYARQFVEQLPRPAIDRLTGIPPTVAIE
                     QRVTRGSRKSTVATITEVAQYLRLLYARLGIQHHPDTDKPVQPLSLGELKSLLKRVLE
                     TPAARRARHLYLCA"
BASE COUNT          203 a          381 c          266 g          193 t
ORIGIN      
        1 gtcgggctcg gctacctcac gctcggccag ccgctcaaca cgctcagcgg cggcgaatcc
       61 caacgtctca aactcgtccg ctacctcggc ggcttcgccg ccgagggttc cgagttcaga
      121 attctgggtt ctgggttgaa gacccaaaac tcaataccca aaacccaaaa ctccgaacct
      181 tgcggcgctc tgctcctgct cgacgaaccg accaccggcc ttcaccgtca cgacgtcaaa
      241 cgcctcttgt ccgtcctgca ggcgctcgtt gatcgcggtc acagcgttgt cgtgatcgag
      301 cacaatctcg atgtgctcaa atccgccgat tgggtcatcg agatcggccc cgacgccgga
      361 gcgaacggcg gccgcgtcgt tgccgaagga acaccggaag acgtcgcccg cgtcgtcacc
      421 gccacctcgc cattcctccg agctgcgctc gccgtttcac cgggtggttc ggagttcggg
      481 gtttcgggtt tggagttagc cgccgccgaa cctgctgcgc cctacatcac ctccacctca
      541 aaactcaaaa ccgaaaaaac agaaacctcc ggctccgagc tccgcatcga gggcgcacga
      601 gaaaacaacc tgaagaatct gtcgctcacg atcccgcatc gtcagctcaa cgtcgtcacc
      661 ggggtttccg gctccggcaa atccacgctg gcgttcgaca tcgtctttgc cgaaggccag
      721 cggcgcttca tggaatcaat gtcgccctac gcgcggcagt ttgttgaaca actgccccgc
      781 cctgcgatcg accggctcac cggcatcccg ccgaccgttg cgatcgaaca acgcgttacc
      841 cgcggttcac gcaaatccac tgtcgcgacc atcaccgagg tcgcgcaata cctccgtctc
      901 ctctacgcgc ggctcggcat tcaacatcat cccgacactg acaaacccgt ccagccgctc
      961 tcgctcggcg agttgaaatc gctcctcaag cgcgtgctcg aaacacccgc ggcccgacgc
     1021 gcgcgtcatc tttatctttg cgc
//
LOCUS       DASPLT010001640         2869 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_20282, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001640 DASPLT010000000
VERSION     DASPLT010001640.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 2869)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2869)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2869
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_20282"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..504)
                     /locus_tag="VEA63_16685"
     CDS             complement(<1..504)
                     /locus_tag="VEA63_16685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007360180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HYD85708.1"
                     /translation="MSTFRVLQFNMQYGQGWDAQDPDNAPIELEATLAEIRRHNADVI
                     LLQEVERAQPGGTQVEPPPNYTRLARALTDYHGWFSYPKADARELPFGIGLAIFSRAP
                     LRDGTRIDLPSPPIAFEFRGEKTTPTDRVLIGAKTTLLGREVQLFNAHLLAFFMLGTT
                     SGEHPAQR"
     gene            complement(511..1083)
                     /locus_tag="VEA63_16690"
     CDS             complement(511..1083)
                     /locus_tag="VEA63_16690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD85709.1"
                     /translation="MKLSGLFFVSHAAAFLFWAATAVHAGEASDYERRNEALAPTASS
                     QLANRADTPAAKTIVTPSPRPPALRAIGSTPAAERAAALRVIEARPKSILARPAKSFG
                     DRAAAREFAVGSPAHYSASDGVKRLPLAGKYQERLSTANRVHIGRTPVLRRGQTASLN
                     RFVFRRKDNASAPVAPQPVVAGGGGSSGER"
     gene            complement(1278..2624)
                     /locus_tag="VEA63_16695"
     CDS             complement(1278..2624)
                     /locus_tag="VEA63_16695"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007360182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050797 - thymidylate synthase (FAD)
                     activity [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HYD85710.1"
                     /translation="MRITGLALVPPPTAADLPKVTPELLASVLARYSRSNEGLATILS
                     KVDLANPDASIDRILKFVDYGHASIGGLTGGLAIALDDVSMWLAYKIFEIATMADGQE
                     SSTRYITMDAANLPTPAELGIPEDLAERWCDVMRQAFASYHSEYARLDALAVANPSLV
                     RLPLDAKPAVVTRLRKNYALDRARYFIPFATRTNLGLVQSSRMWATTVKHLDSLAHPE
                     ARAAAKLIRDELLKQSPRLMRHSSAEKSYTAQAEQELATSLRLGLERLSSAPLADDVW
                     VHVDRATPPWLPETQAVAEALRHRANRYGHQGSATRRMRVSFAWNNMALAELRDLNRH
                     RTGHRYTPLIQAGFYLPPEIKHADHAQLLDAQRELTRELMRRGSPAYVYSLLLGAQTP
                     FEHSTHADKFIYEAELRTGMGAHFRYAEHLSAALKGFLAQVPEARDWVVEGTAEPE"
BASE COUNT          511 a          904 c          967 g          487 t
ORIGIN      
        1 gcgctgcgcg ggatgttcgc cgctcgtggt gccgagcatg aagaacgcga gcaggtgcgc
       61 gttgaaaagc tgcacctcgc gaccgagcag cgtggtcttc gcaccgatga gcacgcgatc
      121 cgtcggcgtg gttttctcgc cgcgaaattc gaacgcgatc ggcggcgacg gcagatcgat
      181 tcgcgtgcca tcgcgcagcg gtgcgcgcga aaaaatcgcg aggccgatgc cgaacggcag
      241 ctcacgcgca tccgctttcg gatacgagaa ccagccgtga tagtccgtca gcgcgcgagc
      301 gagccgcgtg taattcggcg gcggctcgac ctgcgtgccg cccggctgcg cgcgctcgac
      361 ctcctgcaac aggatcacgt cggcgttgtg ccgcctgatt tcggcgagcg tggcctcgag
      421 ctcgatcggc gcattgtccg gatcttgcgc atcccagccc tgcccgtatt gcatgttgaa
      481 ctgaaggacg cggaaggtgc tcatgcgcgt tcaacgttcg ccgctcgatc cgccgccacc
      541 cgccaccacc ggttgcggcg caaccggcgc ggaggcgttg tcttttcgac gaaatacgaa
      601 gcgattcaaa ctcgcggtct ggccgcgacg cagcacaggc gtgcgaccaa tgtgcacgcg
      661 attcgccgtc gacaaacgct cctgatattt tcccgcgagc ggcagccgct tcacgccgtc
      721 gctcgcggag taatgcgcgg gcgatcccac ggcaaactca cgcgccgcgg cgcgatcgcc
      781 aaacgatttt gccgggcgcg ccagaatcga ttttggccgc gcttcgatca cgcgcagcgc
      841 ggcggctcgc tccgccgcgg gcgtcgatcc gattgcccgc aaagccggcg ggcgcggcga
      901 cggcgtgacg atggttttcg ccgcaggagt gtcggcgcgg ttcgcgagct ggctggatgc
      961 cgtgggcgcc agcgcttcgt tgcgtctttc gtaatcgctc gcctcgccgg cgtgcaccgc
     1021 cgtcgcggcc caaaacaaaa acgccgcggc atgggagacg aagaataagc cggacaactt
     1081 catgcgcgag agcggcaagc aagcggccaa ctgcgagcgc agcaatccaa tagtaggcga
     1141 taaattccct gcgaaaaaaa cgccgagatc gagcgaccaa aaaggcggag cccgatgtcc
     1201 tcatcgggct ggtttgaaga gcgacgcgta gcaccaagca agcccgacgg gatgtcgggc
     1261 tccacctcgg tgacgcctca ctccggctct gcggtgcctt cgacgaccca gtcgcgcgct
     1321 tcgggaacct gcgcgagaaa gcccttcagc gcggcgctga ggtgttccgc atagcgaaaa
     1381 tgcgcgccca tgccggtgcg cagttccgct tcgtaaataa acttatccgc gtgcgtgctg
     1441 tgctcgaagg gcgtctgcgc gccgagcagc agcgaataca catacgccgg ggaaccgcgc
     1501 cgcatcagct cgcgtgtgag ctcgcgttgc gcatcgagca actgcgcgtg atcggcatgc
     1561 ttgatttccg gcggcagata aaagccggcc tgaatcagcg gcgtgtaacg atgacccgtg
     1621 cgatggcgat tgagatcgcg cagttcggcg agcgccatgt tgttccacgc gaagctcacg
     1681 cgcatgcggc gcgtcgcgct gccttgatga ccgtagcgat tcgcgcgatg ccgcagcgct
     1741 tcggccacag cctgcgtctc cggcagccac ggcggggtcg cgcgatcgac gtgcacccag
     1801 acgtcgtcgg cgagcggcgc ggacgaaagc cgttcgaggc cgagacggag cgatgtcgcg
     1861 agttcctgct ccgcttgcgc ggtgtaggat ttttccgcgg agctgtggcg catgaggcgc
     1921 ggcgattgtt tcaacagttc gtcgcggatg agcttcgccg ccgcacgcgc ttcggggtgc
     1981 gcgagcgagt cgaggtgctt caccgtcgtc gcccacatgc gcgacgactg gacgaggccg
     2041 aggttcgtgc gcgtggcgaa cgggatgaaa taacgcgcgc gatcgagcgc gtagtttttg
     2101 cgcagacgcg tgacgaccgc gggcttcgcg tcgaggggca gacggacgag cgacggattg
     2161 gcgaccgcga gcgcgtcgag tcgcgcgtat tcggaatggt aggacgcgaa ggcctgccgc
     2221 atcacgtcgc accaccgctc cgcgagatcc tccgggatgc cgagctcggc gggcgtcggg
     2281 agattcgccg cgtccatcgt gatgtagcgc gtgctcgatt cctggccgtc ggccatcgtc
     2341 gcgatctcga aaattttata cgcgagccac attgacacgt cgtcgagcgc gatcgccagg
     2401 ccgcccgtca gcccaccgat cgacgcgtgc ccgtaatcga caaacttcag gatgcgatcg
     2461 atcgaggcat ccggattggc gaggtcgacc ttcgagagaa tcgtcgcgag gccttcgttg
     2521 ctgcgcgagt agcgagcgag caccgacgcc agcaactcgg gcgtgacttt gggcaaatcg
     2581 gcggcggtgg gcggcggaac gagggcgagt ccggtgattc tcatgcgtcg gtgaccatgg
     2641 ccgcaaagag gggcaaaagt cgcaagacgg gaatggttcg aaatccgccg aaggtggagc
     2701 acgacgtccc gtcggattgg ttcgactcat ctgccgcacg ccctcgatca gcccgacgag
     2761 gactcgtcgg acttgtgtag cggcaggcgt ccgtgcctgc ctgctctgtt ttctagacgg
     2821 ataggtggtg cgcttgcgca ccacgttgcg cgcgagcacg caacctaca
//
LOCUS       DASPLT010001641         2070 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_59448, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001641 DASPLT010000000
VERSION     DASPLT010001641.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 2070)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2070)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2070
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_59448"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..227
                     /locus_tag="VEA63_16700"
     CDS             <1..227
                     /locus_tag="VEA63_16700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD85711.1"
                     /translation="EETFGYFCGNNPFDARLFTEIGGVYNFVTDTDGDTIEDRLHFDL
                     YPESTAFVQIGVLRHLEHRIRASLSTQPLP"
     gene            224..>2070
                     /locus_tag="VEA63_16705"
     CDS             224..>2070
                     /locus_tag="VEA63_16705"
                     /inference="COORDINATES: protein motif:HMM:NF024240.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HYD85712.1"
                     /translation="MKKYLHLGLCVFLLCPLFVHAAEPDVVVFGGTSAGVAAAVAAGR
                     NGSKVVLVEPSYLIGGLTTGGLTKTDIGKPATICGLAREFYDRVLAYYKKNYGVDSAQ
                     AKHSGGGYFFETKVALAIYEEMLAEAGVEVRIREQLEDVEVRDHRIVAFTTRNYETGV
                     TTRFTGQLFIDGTYEGDLLARAGVLYRVGREARAEYNEPLAGITVGPDEYVGKGDHRV
                     QAYNIRGTLSVRDDNRVPIPKPKHYYREAHANLIDTVNQLKLTRLDQLFTDTERWAMI
                     NGKCDPNKADFPGVNFGYVEGDYEQRARITAKVQDYWLSLWWMLQNDPALPEEFKADA
                     RRWGLPKDEFVESGHVTPQVYVRVGRRMLGRYHLTQRDLLYDRFKPDTVCMGSYNMDA
                     HAIHSIWTDEGLKEEGHFNDASDAYEIPYRSLLPYGVDNLVVVAAVSATHVAYSSLRM
                     EPVFMMLGHAGGLAVDLARSSGARVQDVSVPELQARLRRDGVPLKAPFRPVVEIKVKT
                     KDPRVGEPVEFELVEKHIESPLKRIAWNFDGSGEVQAAGKTARYTFNQASPATVTVLV
                     QDEKRLLALPASIEVPLGDEPPLDREVHYMKAKLDGRWNRARGPEVEYR"
BASE COUNT          389 a          592 c          704 g          385 t
ORIGIN      
        1 tcgaggaaac gttcggttac ttctgcggga acaatccatt cgacgcgcgg ctcttcaccg
       61 aaatcggcgg cgtctacaat ttcgtgaccg acacggacgg cgacacgatc gaggaccggc
      121 tgcatttcga tctttatccc gagtcgacgg cgttcgtgca gatcggcgtg ctgcgccatc
      181 tggaacatcg catccgtgca tccctttcaa cccaacccct gccgtgaaaa aatacctcca
      241 tctcggtctt tgtgtttttc tgctttgtcc tttgttcgtc catgcggccg agcccgacgt
      301 cgtcgtattc ggcggcacgt ccgcgggtgt cgccgctgcc gtcgctgcgg ggcggaatgg
      361 ttcaaaggtg gtgctggtcg aacccagcta tttgatcggc ggactcacca cgggtgggtt
      421 gacgaagacg gacatcggca agccggcgac catctgcggg ctcgcgcgtg aattctacga
      481 ccgcgtgctg gcgtattata aaaagaacta cggcgtggat tccgctcagg cgaagcacag
      541 cggcggcggt tattttttcg agacgaaggt ggcgctcgcg atctatgaag agatgctcgc
      601 cgaagccggc gtggaggtgc ggatccggga gcagctcgag gacgtcgagg tgcgcgatca
      661 ccggatcgtc gcgtttacca cgcgcaacta cgagacgggc gtcacgacgc gcttcaccgg
      721 gcagttgttc atcgacggca cttatgaagg cgacctgctg gcgcgggcgg gcgtgcttta
      781 tcgcgtcggt cgcgaggcgc gggcggaata caacgagccg ctcgctggca tcacggttgg
      841 gccggacgaa tacgtcggca aaggcgatca tcgcgtgcag gcctataaca ttcgcgggac
      901 gctgagcgtt cgcgacgaca accgcgtgcc gatcccgaag ccgaaacatt attaccgcga
      961 ggcgcacgcg aacctgatcg acacggtgaa tcagttgaag ctcacgcggc tcgaccagct
     1021 gttcaccgac acggagcgct gggcgatgat caacggcaag tgcgatccca acaaggcgga
     1081 cttcccgggg gtgaatttcg gctacgtcga aggcgactac gaacagcgcg cgcgcatcac
     1141 cgcgaaggtg caggactact ggctgagcct ctggtggatg ctgcagaacg atccggcgct
     1201 gccggaggaa ttcaaggcgg acgcgcgtcg ctggggtttg ccgaaggacg agtttgtcga
     1261 gagcggccac gtgacgccgc aggtttatgt gcgcgtcgga cggcggatgc tcggccgcta
     1321 ccatctcacg cagcgggatc tgctctacga ccgcttcaag cccgacacgg tttgcatggg
     1381 cagctacaac atggatgcgc atgcgatcca ctcgatctgg accgacgagg gattgaaaga
     1441 ggaggggcat ttcaacgatg cgtccgatgc ctacgagatt ccgtatcgca gcctgctgcc
     1501 ctacggtgtc gacaatctcg tggtggtcgc ggcggtgtcg gcgacgcatg tcgcttactc
     1561 gagcctgcgg atggagcctg tattcatgat gctgggacac gcgggcgggc tcgcggtcga
     1621 tctggcgcgc tcgagcggcg cgagggtgca ggacgtgtcg gtgccggagc tgcaggcgcg
     1681 gctgcggcgc gacggtgtgc cgttgaaggc gccgttccgc ccggtggtgg agatcaaggt
     1741 caagacgaag gatccgcgcg tcggcgagcc ggtggagttc gaactggtgg agaagcacat
     1801 cgagtcgccg ttgaagcgca tcgcgtggaa tttcgacggc agcggcgagg tgcaggcagc
     1861 agggaagacc gcgcgttaca cgttcaacca ggcgtcaccg gcgacggtga ccgtgctcgt
     1921 gcaggacgaa aagcgtctgc tggcgttacc cgcctcgatc gaagtgcctc tgggtgacga
     1981 accgccgctc gatcgcgagg tgcattacat gaaggcgaag ctcgacggtc gttggaaccg
     2041 cgctcgcggt cctgaggtgg agtatcgtca
//
LOCUS       DASPLT010001642         1175 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_163646, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001642 DASPLT010000000
VERSION     DASPLT010001642.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1175)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1175)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1175
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_163646"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            111..>1175
                     /locus_tag="VEA63_16710"
     CDS             111..>1175
                     /locus_tag="VEA63_16710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015486 -
                     glycoside-pentoside-hexuronide:cation symporter activity
                     [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide (GPH):cation
                     symporter"
                     /protein_id="HYD85713.1"
                     /translation="MIQEKLPLREKLAYGCGDFASCLYWTTFMRYLPFFYTDVFGISA
                     AALGTMLLVSRMWDGVNDPIIGMFADRTDTRWGKFRPFILFGAVPFAIFGVLTFTTPD
                     LGASGKLIWAYLCYNGLMMLYTTVNIPYTAMLGVMTTTAAERTRLSSVKFIFAFSAGI
                     VVSATLLPLVEWLGGDNAQLGWQLAFVVYGVVAIGFFLITFAGTRERIKPAPDQNTSV
                     LRDIGFLLSNKAWLLLTATTLTWILFVALRSSVSAHYFKYYLYNGEPDRALSFLGHDF
                     TLTGLLSAFNTLGQTASVVGVLLTSVVASRFAKRPLFIAFFVVSIACTAAYYVLPPGQ
                     LGIIFTLEALGSLAGAPLPVL"
BASE COUNT          178 a          418 c          300 g          279 t
ORIGIN      
        1 cacctcgaga ctcgccgcgc tttcaggctg ggcttaacca ttcccgccac cttatctcgt
       61 gcacccgctc gcgacgccct cccccgacgc gccgcacccc agacttcacc atgattcagg
      121 aaaaactccc gcttcgcgag aagctcgcct acggctgcgg tgacttcgcg tcgtgcctct
      181 attggacgac gttcatgcgc tacctgccgt ttttctacac cgatgtcttt ggcatctccg
      241 ccgccgcgct cggcacgatg cttctcgtta gccgcatgtg ggatggcgtc aacgatccca
      301 ttatcggtat gttcgccgat cgcaccgaca cgcgctgggg gaagttccga ccgttcatcc
      361 tgtttggcgc tgtccctttc gcgattttcg gtgtgctcac gttcacgaca cccgatctcg
      421 gcgcgtcagg aaaactcatc tgggcctatc tctgctacaa cggcctcatg atgctctaca
      481 cgaccgtgaa catcccctac accgcgatgc tgggcgtgat gaccaccacc gccgcggagc
      541 gcacacgctt gtcctccgta aagtttattt tcgcgttctc cgccggtatc gtggtttccg
      601 caacgctgct gcccctcgtg gaatggctcg gcggcgacaa cgcccaactc ggttggcagc
      661 tcgccttcgt cgtctacggt gtcgtcgcga tcggtttctt cctcatcacc ttcgccggca
      721 ctcgcgaacg gatcaaaccc gcacccgatc aaaacacatc ggtgctccgc gatattggtt
      781 tccttctgtc gaacaaagcc tggctgctgc tcacggcgac gacgctgacg tggatcctgt
      841 tcgtcgcgtt gcgcagttcg gtctcggccc actatttcaa atactacctc tacaacggcg
      901 agccggatcg cgcgctttcg ttcctcggcc acgatttcac gctcacgggc ctgctctctg
      961 cattcaacac cctcgggcag accgcgtccg ttgtcggtgt tctgctcacc agcgtcgtcg
     1021 ccagccgttt cgccaagcgt ccgctcttca ttgcgttctt cgtcgtgtcg atcgcatgca
     1081 ccgccgctta ctacgtgctg ccccctggtc agctcggaat catcttcacg ctcgaggcgc
     1141 tcggcagtct cgcgggtgcg ccgctgccgg tgctg
//
LOCUS       DASPLT010001643         1072 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_244253, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001643 DASPLT010000000
VERSION     DASPLT010001643.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1072)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1072)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1072
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_244253"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..743
                     /locus_tag="VEA63_16715"
     CDS             <1..743
                     /locus_tag="VEA63_16715"
                     /inference="COORDINATES: protein motif:HMM:TIGR02276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD85714.1"
                     /translation="EIVSTLEIGPLCNHVALVDTPRGAFAYLTVGGLDEVQVWTREAE
                     PKRVARIPTGELPHGIWPAPDGSRVYVSLENGDAVQAIDTATNEIVATIGVGHLPQAI
                     VYVPGVGAPERGSEALRDLSSIGRPEHLRLAPTEHAPAGARGNVVLNPLGQIDHLQVV
                     AVGLEPENDYELTLASPANAASEAPVPLARAKANPAGAVVMQALGPLRKVAGASGGRE
                     RPASASAVRTVELRSVKEGRTVLVQARR"
     gene            complement(751..>1072)
                     /locus_tag="VEA63_16720"
     CDS             complement(751..>1072)
                     /locus_tag="VEA63_16720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD85715.1"
                     /translation="GLQRDADPLPSLPRLSAHVDPAHDNLSFIERAQTHQALERGRLS
                     STVWSKQSENFPASDLKAHPAQRFHAIVALGEILDGDSDIHAIKDDTPDTFAPLEQQA
                     LFSV"
BASE COUNT          195 a          289 c          399 g          189 t
ORIGIN      
        1 tcgagatcgt ctcgacgctg gagatcgggc cgctttgcaa ccacgtcgcc ttggtggaca
       61 cgcctcgggg cgcgttcgcg tatctgacgg tgggcggatt ggacgaggtt caagtgtgga
      121 cgagagaggc agaaccaaag cgcgtcgcgc gaatcccgac cggtgagctg ccgcacggaa
      181 tctggccggc gccggatggc agccgcgttt atgtgtcgtt ggaaaatggt gacgcggtgc
      241 aggcgatcga cacggcgacg aacgagatcg ttgcgacgat tggcgtgggg cacttgccgc
      301 aggcgatcgt ctatgtgccg ggagtaggtg cgccggagcg cggatcggag gcgctgcgcg
      361 atttgagcag catcggacgt ccggaacatc tgcgcctcgc gccgacggaa catgcgccgg
      421 cgggtgctcg cggaaatgtg gtgctgaacc cactggggca gattgatcat ctacaggtgg
      481 tcgccgtcgg gttggagccg gaaaacgact acgagctcac gcttgcatcg ccggcgaatg
      541 ccgcatccga ggcgccggtc ccgcttgcgc gcgcgaaggc gaatccggcc ggtgcagtcg
      601 tgatgcaggc gctcggcccc ttgagaaagg tagcaggagc gagcggtgga cgtgagcggc
      661 ctgcttctgc cagcgcggtc cgcacggtgg agctgagatc ggtgaaggaa gggcggacgg
      721 tgctcgtgca ggcgcgacgt tgatggcgcg ttacacagag aaaagggctt gttgctccaa
      781 cggcgcgaac gtatcaggcg tgtcgtcttt gatcgcatgg atgtcgctgt ctccgtccaa
      841 aatctctcca agcgctacga tcgcgtggaa gcgctgcgcg gggtgagctt tgaggtccga
      901 cgcggggaaa ttttcggact gcttggacca aacggtgctg gaaagacgac cacgctcgag
      961 tgcttggtgg gtctgcgcac gttcgataaa ggaaagattg tcgtgagcgg gatcgacgtg
     1021 cgcgctcagc cgcgggaggc tcggcagcgg atcggcgtcg cgctgcaaac ca
//
LOCUS       DASPLT010001644         1242 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_204725, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001644 DASPLT010000000
VERSION     DASPLT010001644.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1242)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1242)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1242
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_204725"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..361)
                     /locus_tag="VEA63_16725"
     CDS             complement(<1..361)
                     /locus_tag="VEA63_16725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HYD85716.1"
                     /translation="MNTSPPSSRPPPPHGRGAGINPPNRFEALHVEADPDCPEEERPN
                     PRTQFFYDATESLLTKNNSPDVGFSYGFNVYRGCEHGCAYCFARPYHEYLGWSSGLDF
                     ETKILVKLRAPELLRREL"
     gene            675..>1242
                     /locus_tag="VEA63_16730"
     CDS             675..>1242
                     /locus_tag="VEA63_16730"
                     /inference="COORDINATES: protein motif:HMM:NF024579.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD85717.1"
                     /translation="MLLVTFAARSSGQESETLADRTFRQLAEEQKALLAGAAADNPNF
                     DMENFRQQIERLCRGYEEFLRANPKSAEGFAAYGYLLSKVDQRKESIAMLLKANQLDP
                     SLPLVKNQIGNYLAEEGRPLDAVAYYMAAIKLAPDEPLYHYQLGSLLTEARDDFLKSG
                     EWTRTAIDEAMHTAFRRATELAPDRFEFA"
BASE COUNT          224 a          357 c          429 g          232 t
ORIGIN      
        1 acaactcgcg gcgcagcagc tcgggcgcgc ggagtttcac gaggatcttc gtctcgaaat
       61 cgaggccgct gctccagccg agatattcgt ggtagggtcg ggcgaagcaa taggcgcagc
      121 cgtgttcgca gccgcggtag acgttgaagc cgtagctgaa gccgacgtcg gggctgttgt
      181 tcttcgtcag gagcgactcg gtggcgtcgt agaaaaactg cgtgcgcgga ttcggccgct
      241 cctcttcggg acaatccgga tcggcttcga cgtgcagcgc ttcgaaccgg ttcggcggat
      301 tgatgcccgc gccgcgacca tgcggcggcg gggggcgcga cgatggcggc gatgtgttca
      361 cctgaacaca tgaggacggt tttcaacgcg cggcaaacgg ttgcgtgagg tgcgccgtcg
      421 ctcgcgatgt aaaccgctgc gaacgtatgc gctcgcgtcg aaaattttcg cggcaccggg
      481 tgttcgcagg ttgcgcgggc acccgggaag gttgagcgtg tcgcactcgg ccgatgaatt
      541 ttcccttggt ggcagtgcga gatttttttt tgcggacgcg tgcgagtcac cgtgcgcgtg
      601 cgcgctcacg cacacacatg cgcgagcgtg agagtgaccc cggcggtcgg acgtcggccg
      661 cattcttggt tacggtgctg ttagtgacgt tcgcggctcg atcgtcgggg caggagagcg
      721 agacgctggc ggaccggaca tttcggcagc tcgcagagga gcagaaggcg ctactcgccg
      781 gcgcggcggc ggacaacccg aacttcgaca tggaaaattt ccgccagcag atcgagcggc
      841 tgtgtcgcgg ttacgaggaa tttctccgcg cgaatccgaa gtcggcggaa gggttcgccg
      901 cctacggcta tctgctctcg aaggtcgatc agcgaaagga gtcgatcgcg atgttgctca
      961 aggcgaacca gctcgatccg tcgctgccgc tggtgaaaaa ccagatcggc aactatctcg
     1021 ccgaggaagg gcggccactc gacgccgtcg cttattacat ggcggccatc aagctcgcgc
     1081 cggacgagcc gctttatcac taccagctcg gttcgctgct aacggaggcg cgcgacgatt
     1141 tcctgaaaag cggcgagtgg acacgcacgg cgatcgacga agcgatgcac acggcgtttc
     1201 ggcgcgcgac ggagctcgcg ccagaccgtt tcgagtttgc gt
//
LOCUS       DASPLT010001645         1174 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_204728, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001645 DASPLT010000000
VERSION     DASPLT010001645.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1174)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1174)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1174
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_204728"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(57..>1174)
                     /locus_tag="VEA63_16735"
     CDS             complement(57..>1174)
                     /locus_tag="VEA63_16735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PatB family C-S lyase"
                     /protein_id="HYD85718.1"
                     /translation="KYAGRDVLPLWVADMDFKVAPAIVEGLHRRVDHAIFGYARPVKS
                     TVDAVVEMFEKRHGWRIDPAWIVWLPGLVVGLNITAQAFAGHGDEVLTLTPVYPPFMT
                     GPRNSGRVPVQVPFALNQAQRRWEIDWDALERAVTPRTRAFFLCNPHNPIARVWRRDE
                     LVRLGEFCVRHNLVLCSDEIHCDLILDAALAHVCTGSLGGEIARRTITLTAPSKTYNV
                     PGLGTSIAVIPDAALRAQFVRATAGIVAEVTSLGFSACEAAYREGEPWRRELVAYLRS
                     NRDFLVDFVARELSGVTIEAPIEATYLAWFNVAALNLADPIAHFEAHGVGLSDGAFFG
                     SPKGRHVRLNFGCPRATLHEALTRMKRALSSLPQKSS"
BASE COUNT          230 a          396 c          355 g          193 t
ORIGIN      
        1 cataatggat gagataatgc gtccacagcc tcgttgtaac ctaatgggtt acaatgtcag
       61 ctactctttt gcgggaggct cgagagggcg cgtttcatcc gggtgagggc ctcgtgaagg
      121 gttgcgcgcg ggcaaccgaa gttcagccgg acgtggcgcc ccttgggaga gccgaaaaag
      181 gcgccatcgc ttagccccac cccatgcgcc tcgaaatgcg cgattgggtc ggcgagattc
      241 agcgcagcca cattgaacca cgcgagatag gtagcttcga tcggcgcttc aatcgtcacg
      301 ccacttagct cgcgcgcgac gaaatccacc aaaaaatcgc gattgctccg caggtaggca
      361 accagctcgc gccgccacgg ctcgccttcg cggtaagccg cttcgcacgc ggaaaacccc
      421 agcgacgtca cctcggccac gatgccggct gtcgcgcgca cgaactgcgc tcgcaacgcc
      481 gcatccggga tcaccgcgat cgacgtgccc agccccggca cgttgtaagt tttgctcggc
      541 gcagtgagcg tgatcgtccg tcgcgcgatc tcgccgccga ggctcccggt gcaaacgtgc
      601 gcgagggccg cgtcgagaat caggtcgcag tgaatctcat cggagcagag caccagattg
      661 tgccgcacgc agaattcccc caggcgcacc aactcgtccc gtcgccagac acgcgcgatc
      721 gggttgtggg gattgcagag aaaaaacgcc ctcgtgcgcg gcgtcaccgc gcgctcgagc
      781 gcgtcccaat cgatttccca gcggcgctga gcctgattga gcgcgaacgg cacttgcacg
      841 gggacgcgac cgctgttgcg cggacccgtc atgaacggcg gatacacggg ggtaagcgtc
      901 agcacttcgt cgccgtgccc ggcgaacgcc tgcgcggtga tattcaggcc aacgaccagc
      961 cccggcagcc agacgatcca cgctggatcg atacgccagc cgtgccgttt ctcgaacatc
     1021 tccaccactg catccacggt ggacttcacc gggcgcgcgt atccgaaaat cgcgtgatcc
     1081 acgcgccggt gcagcccctc cacgatggct ggcgcaacct tgaaatccat gtcggccacc
     1141 cacagcggaa gcacgtcgcg gcccgcgtat ttct
//
LOCUS       DASPLT010001646         1069 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_244261, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001646 DASPLT010000000
VERSION     DASPLT010001646.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1069)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1069)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1069
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_244261"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            136..951
                     /locus_tag="VEA63_16740"
     CDS             136..951
                     /locus_tag="VEA63_16740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HYD85719.1"
                     /translation="MSVETRHLRSGYKPRKLATPRGPTTVALMIEVSGLTKRYGEFTA
                     VRELSFVVQPGQVLGLVGPNGAGKTTTLRCTTGIIPSTTGTVRLAGHDLAAEPVAAKR
                     ALAFFPDEPRLFEYLTVRQHLAFVARVYGVKDHETVAQPLLEEFEIADKANELPGALS
                     RGMKQKLAIACGLLHAPSVLFFDEPLTGLDPLGIRRMKDSILRRARAGAAIVLSSHLL
                     HLLQEVCSHVLILQRGEKIADGTIPEVAARFSHGDTNVNLEEIFLRATGGATQ"
BASE COUNT          180 a          364 c          317 g          208 t
ORIGIN      
        1 cgcgcatttc ttttcctcgt ggacgggcgg gttgctccgc agacgaagag gtttcggtcg
       61 ccgcgcgagt cgcggggctc caggccagca cggccagcat cacgagcgag aaaaatttgc
      121 gcgaggtttt catgggtgag cgtcgagacg cgacatctcc gaagcggtta caagccgcgc
      181 aagcttgcca cgcctcgcgg cccgaccacc gtcgcgctca tgatcgaagt gtcgggcctc
      241 accaagcgct acggcgagtt tacggcggtg cgcgagctat cgtttgtcgt ccaacccggt
      301 caggtgctcg gtctggtcgg ccccaacggc gccggcaaaa ccaccacgct gcgctgcacc
      361 accggtatca ttccttcaac tacgggcact gtccgtctcg ccggccacga tctggccgca
      421 gagccggtcg ccgcaaaacg cgccctggcg ttttttccgg acgagccgcg tctcttcgaa
      481 tatctcaccg tccggcagca cctcgcgttc gtggcgcgcg tttacggcgt gaaggaccac
      541 gagaccgtcg cgcaaccgct gctcgaggaa ttcgagatcg cggacaaggc caacgagctg
      601 ccgggcgcac tttcgcgggg catgaaacag aaactcgcga tcgcctgtgg tctgttgcat
      661 gcgccgtccg tgttgttttt cgacgagccg ctcacggggc tcgatccgct cggcatccgg
      721 cggatgaagg actccatcct ccgccgggcg cgagctggcg ccgcgatcgt gctcagctct
      781 catctgctgc acctccttca agaagtttgc tcgcacgtgc tgatcttgca gcgcggtgaa
      841 aaaatcgcgg acggcaccat tccggaagtc gccgcgcgct tcagccatgg cgacacgaac
      901 gtgaatctcg aggaaatctt cctccgcgcc accggcggcg ccactcagta gcggcgcgct
      961 cttcgccgtc tctcgcgtcc catgcttcgc gcaattcttt acctgcgtct cacctcgttg
     1021 aagaacttgc tgttctcgcg acttcgccgg ctgcgtcagc ccaaatacc
//
LOCUS       DASPLT010001647         2459 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_100913, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001647 DASPLT010000000
VERSION     DASPLT010001647.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 2459)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2459)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2459
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_100913"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(82..1629)
                     /locus_tag="VEA63_16745"
     CDS             complement(82..1629)
                     /locus_tag="VEA63_16745"
                     /inference="COORDINATES: protein motif:HMM:NF024120.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 28-related protein"
                     /protein_id="HYD85720.1"
                     /translation="MRPLPLLASLFFIASPVAFCADAAAAADPAPQRYSELWGRAGEK
                     WTPTSRLPDFSFAGYRFGEVPLPHVPVAANVRDFGAKGDGEHDDTQAFKAAIAQTSSG
                     AIFIPPGCYVITDILWIRKPNLVLRGAGTDRTILVCPKPLEHIAPNTGATTTGRPTSN
                     YSWSGGIIWVRGSRESRDARAVAADALRGDTVITVASADNLSPGQRVILHLQDDTART
                     LIDHLYAGDPGDTSKITKPITTSVVSRVVSIDGQRVTLERPLPFDVRAAWTPTLSVFD
                     PDVSEVGIEAIRFEFPITPYEGHFTEPGYNAIAIDRASDCWVRDIHVQNADSGVFLRG
                     TFCTLDGFVLDSDRPLNVARQATGHHALNMGVDCLAQNFDFRTHFIHDITVDSGDVRN
                     VVKNGRGINLSLDHHKRVPYGNLFTNLDAGDGSELWHCGGGAALGKHCAAWGTFWNIR
                     TAKPVPSPDKNFAPPTINLVGLHIDEPTQTDPDGRWFEAIPPGELYPADLHAAQLARR
                     LGRAAKP"
     gene            complement(1690..>2459)
                     /locus_tag="VEA63_16750"
     CDS             complement(1690..>2459)
                     /locus_tag="VEA63_16750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD85721.1"
                     /translation="LNGRVQGSVHVLTAESITLNSSASISGDLLLPGTPAVKLNGQPN
                     FGGAIDGPGASAPANATVTLNSQATLRHLVRRIDPIALPVVSAPPAPSGTRNVSLNNT
                     TQDAGEFTTVRNLTLNSNAGMRAIPPGTYGALTANGSSGFILGVAGATEPAIYNLQNL
                     TVNGNSTVQIVGPVVLTLANGAHLNGVIGSADAPERLLLRIASGGLTLNGGAALHGFV
                     DAPNGTITINGNATLNGGLTSDRLIVNGNGLLIADAR"
BASE COUNT          417 a          652 c          958 g          432 t
ORIGIN      
        1 gcacggaggc ctgccgctac cttgcgcaag ccgaactcat gcggcagaag gtggagcgcg
       61 aggctttagt tcggtttccg tttacggctt cgcggcgcgg ccgaggcggc gggcgagttg
      121 ggcggcgtgc aggtcggcgg gataaagttc gccgggcggg atcgcttcga accagcggcc
      181 gtcgggatcg gtttgcgtgg gctcatcgat gtggagaccg acgagattga tcgtgggcgg
      241 cgcaaaattc ttatcggggc tgggcaccgg cttcgcggtg cgaatgttcc agaaggtgcc
      301 ccatgcggcg cagtgtttgc ccagcgcagc gccgccaccg caatgccaga gctcggagcc
      361 gtcgccggcg tcgagattgg tgaagagatt gccgtagggg acgcgcttgt ggtgatcgag
      421 cgagagattt atgccgcggc cgtttttcac gacattgcgg acgtcgccgc tatcgacggt
      481 gatgtcgtgg atgaagtgcg tgcgaaaatc gaagttctgc gcgaggcagt cgacgcccat
      541 gttcaacgca tgatgaccgg tcgcctggcg ggcgacgttg agcgggcgat cggagtccaa
      601 cacgaaaccg tcgagcgtgc agaacgtgcc gcgaagaaat acgccgctgt cggcgttttg
      661 cacgtggatg tcgcggaccc agcaatcgga agcgcgatcg atcgcgatgg cattgtaacc
      721 gggttcggtg aagtggcctt cgtagggtgt gatcggaaac tcgaaacgaa tcgcctcgat
      781 gccgacttcg ctcacatcgg gatcgaacac gctgagcgtc ggcgtccatg cagcgcgcac
      841 gtcgaagggg agcgggcgct cgagcgtcac gcgttgaccg tcgatggaca ccacgcggct
      901 gacgacgctc gtggtgatgg gttttgtgat cttggaggtg tcgcccggat cgccggcgta
      961 gagatgatcg atcagcgtgc gcgcggtatc gtcttgcagg tggagaatga cgcgttgacc
     1021 gggcgagagg ttgtcggcgg aagcgacggt gatgacggta tcgccgcgga gcgcgtcagc
     1081 ggcgacggcg cgagcgtcgc gcgattcgcg gctgccacgc acccaaatga tgccgccgga
     1141 ccaggagtag ttggaggtgg ggcggcccgt ggtggtcgcg ccggtgtttg gcgcgatgtg
     1201 ttcgaggggt ttggggcaaa cgagaatggt gcggtcggtg ccggcaccgc gaaggacgag
     1261 gttgggtttt cgaatccaga ggatgtcggt gatgacgtag cagccgggcg gaatgaagat
     1321 cgcgccggag gaggtttgcg cgatggcggc cttgaacgcc tgggtgtcgt cgtgttcgcc
     1381 gtcgcccttc gcgccgaagt cgcgcacgtt cgccgcaacc ggaacgtggg gcagcggcac
     1441 ttcgccgaag cgataaccgg cgaaggagaa atcggggagg cggctcgtgg gcgtccattt
     1501 ctcgccggcg cggccccaaa gttcggaata gcgctgcggc gcggggtccg ctgcagcggc
     1561 tgcgtcggcg caaaatgcca ccggagatgc aatgaagaag agcgaggcga ggagcgggag
     1621 agggcgcatt gctgaaaaac aaaagggaat cggctcgcga tcaggaagga tcgcgagccg
     1681 gcagtttgat caccgggcgt ccgcgatcaa caagccattg ccgttgacga tgaggcgatc
     1741 gctggtgagg ccgccgttga gggtggcgtt gccgttgatc gtgatcgtgc cgttgggggc
     1801 gtcgacaaaa ccgtgcagcg cggcgccgcc gttgagcgtg aggccgccgg aggcgatgcg
     1861 aaggagcagg cgttcgggcg cgtcggcgga accgatcacg ccgttcaggt gtgcgccgtt
     1921 ggcgagcgtg aggacgacgg ggccgacgat ctgcaccgtg ctgtttccgt tgaccgtgag
     1981 attttgcagg ttgtagatcg cgggctcggt ggcgccggcg acgccgagga tgaaaccgct
     2041 gctgccgttg gcggtgagag cgccgtaggt gccgggcgga atcgcgcgca tgccggcgtt
     2101 gctgttgagc gtgaggttgc gcacggtggt gaactcgccg gcgtcctgcg tcgtgttgtt
     2161 cagcgaaacg ttgcgggtgc cgctcggtgc gggcggcgcg cttacgaccg gcagcgcgat
     2221 cgggtcgatg cggcgcacga ggtgacggag cgtggcctgg ctgttcaacg tgacggtggc
     2281 gttcgcggga gccgaagcgc cggggccgtc aatcgcgcca ccgaagttcg gctgaccgtt
     2341 gagcttcacg gcgggtgtgc cgggaaggag caaatcgccg gagatggagg cgctgctgtt
     2401 gagcgtgatg gactcggcgg tcaacacgtg cacggagcct tgcacgcggc cgttgagga
//
LOCUS       DASPLT010001648         1250 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_163675, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001648 DASPLT010000000
VERSION     DASPLT010001648.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1250)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1250)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1250
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_163675"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..285)
                     /locus_tag="VEA63_16755"
     CDS             complement(<1..285)
                     /locus_tag="VEA63_16755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003328273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD85722.1"
                     /translation="MSTEPTSAPATAAASETAAPAAAPSAPTRQPRQFNRGPNRGGPN
                     RGGPGGQRRDNRDNRDQGDGPQMIEKVVFINRCAKVVKGGRRFSFAALAVV"
     gene            complement(294..659)
                     /gene="rplR"
                     /locus_tag="VEA63_16760"
     CDS             complement(294..659)
                     /gene="rplR"
                     /locus_tag="VEA63_16760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006784446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HYD85723.1"
                     /translation="MSNTIRKADLLQKRRWRIRKKVQGTALRPRLAVRFTSKHIYAQA
                     IDDDKGATLVFLGSLDAELRKQKLAANLAGAKALGVAFAAKAKAAGIESVVFDRSGAP
                     YHGKVKTFAEAAREGGLQF"
     gene            complement(710..>1250)
                     /gene="rplF"
                     /locus_tag="VEA63_16765"
     CDS             complement(710..>1250)
                     /gene="rplF"
                     /locus_tag="VEA63_16765"
                     /inference="COORDINATES: protein motif:HMM:TIGR03654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HYD85724.1"
                     /translation="MSRIGKQPVSIPDKVKVTVNKDGTVLVEGPKGKVQKNFASAVKV
                     TVGDKKITFAPTEETRFANAMYGTARSVVAGMVKGVTEGYVKDLEIQGVGFKANLKGK
                     QLDLALGYSHPILMDIPEGIKVTVTDQTKLKVEGADKQLVGAITAEIRSYYPPEPYKG
                     KGVRIVGERVRRKEGKTVA"
BASE COUNT          196 a          342 c          432 g          280 t
ORIGIN      
        1 gacaacggcg agagcggcga agctgaaacg gcggccgcct ttaacgacct tggcgcagcg
       61 attgatgaaa acgaccttct cgatcatctg cgggccgtcg ccttgatcgc ggttgtcgcg
      121 attatcgcgg cgctggccgc cagggccacc ccggttcgga ccgccgcggt tggggccgcg
      181 gttgaattga cgaggctggc gcgtcggagc ggaaggagcg gcggcgggag cggctgtctc
      241 agaggcagcg gcggtggcgg gcgcggaagt gggttcggtg ctcatgcttg tttttagaat
      301 tggagaccac cttcgcgggc ggcttcggcg aacgttttga ctttgccgtg atacggcgcg
      361 ccgctgcggt cgaagacgac cgattcgata cctgcggcct tggctttggc ggcgaaggcg
      421 acgccgagcg ctttcgcacc tgcgaggttg gcggcgagct tctgcttgcg cagctcggcg
      481 tcgagcgatc cgagaaacac gagcgtggcg cccttgtcgt cgtcgatcgc ctgagcgtag
      541 atgtgcttcg aggtgaagcg caccgcgaga cgcggacgaa gtgcggtgcc ttgaactttt
      601 ttgcggattc tccagcgacg tttctgcagg agatcggctt tgcgaatggt attgctcacg
      661 gaatctgaaa tttgagattt gcgatttgag attcgcggcg aagccgcgat taggcgacgg
      721 tcttgccttc cttgcggcgg acgcgctcgc cgacgatgcg cacgcctttg cccttgtaag
      781 gttccggcgg atagtagctg cggatttccg cagtgatggc gccgacgagc tgcttgtccg
      841 caccttcgac cttaagcttg gtctggtcgg tgacggtgac tttgatgccc tccggaatat
      901 ccatcaggat cgggtgcgag tagccgagcg cgaggtcgag ctgcttgccc ttgaggttcg
      961 ccttaaaacc gacgccttgg atttcgaggt ccttgacgta gccttccgtg acgcccttga
     1021 ccatgccggc gacgacggag cgcgcggtgc catacatggc gttcgcgaaa cgggtctcct
     1081 ctgtcggagc gaaggtgatt ttcttgtcgc cgactgtcac tttgacggcg gaggcgaagt
     1141 tcttctgaac tttgcccttg gggccctcga cgagcacggt gccgtctttg ttgacggtga
     1201 ccttgacctt gtcggggatg gaaacgggtt gtttgccgat gcggctcatg
//
LOCUS       DASPLT010001649         1769 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_225064, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001649 DASPLT010000000
VERSION     DASPLT010001649.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1769)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1769)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1769
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_225064"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..880
                     /locus_tag="VEA63_16770"
     CDS             <1..880
                     /locus_tag="VEA63_16770"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008740 - L-rhamnose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HYD85725.1"
                     /translation="GLDFNPTYFAHPNAASGFTLSHADSSIRNFWIAHAKACRRIAQH
                     FAKKLGSPCVHNHWVPDGAKDAPADRWAPRERLAASYDAIFADKSISRKLCVDAVEGK
                     LFGLGSEDYVVGSQEFYSSYAQSRDLVLCLDLGHYHPTESVADKVSALMQFHPRVLLH
                     TSRPIRWDSDHVVILDDAVRALFLEIARGDAWNRVFVALDFFDASINRIAAYVVGARA
                     TRQAILLGLLDPSKQLRTAESANRGHERLALMETAKALPWGAVWDELCARDNVPARAD
                     WLAAVATYEKQVLSQR"
     gene            complement(1003..>1769)
                     /locus_tag="VEA63_16775"
     CDS             complement(1003..>1769)
                     /locus_tag="VEA63_16775"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HYD85726.1"
                     /translation="VFWNVLKNAVKFTPQRGAISVTSRTENAQLHIDISDTGIGMTED
                     EIGRSFKSFSQGDHALRHSTHRFGGLGLGLAISRMLMELHGGEISARSAGRGRGATFC
                     IRLPVVDPPKPENRVEEPANVTNGAEQTGEGTRILLVEDHEPTRMAVTRLLGRRGYRV
                     KSATNAAEAIRLAAASTFDLVISDIGLPDIDGYALMRQLRERHRLRGVALSGYGMEDD
                     IARGRAAGFEEHLTKPIDIKKLQAALERMRACNDRS"
BASE COUNT          307 a          650 c          458 g          354 t
ORIGIN      
        1 cggcctcgat ttcaacccca cctacttcgc gcatcccaac gccgcgtccg gcttcaccct
       61 ctcgcacgcc gacagctcga ttcgcaactt ctggatcgct catgccaaag cctgtcgtcg
      121 catcgcccaa cacttcgcga aaaaactcgg ctcaccctgc gtccacaatc actgggttcc
      181 cgacggtgca aaagacgcgc ccgccgatcg ctgggcgccg cgcgaacgcc tcgccgcttc
      241 ctacgacgcg atcttcgctg acaaatccat cagccgaaaa ctctgcgtcg atgcggtcga
      301 aggaaaactc ttcggcctcg gcagcgaaga ctacgttgtc ggctcccagg aattttattc
      361 ctcctacgcg caatcccgcg acctcgttct ctgcctcgat ctcggccatt accacccgac
      421 ggaatccgtt gccgacaaag tctccgcgtt gatgcagttc catccgcgcg tccttcttca
      481 cacctcacgc ccgattcgct gggatagcga tcacgtcgtc atcctcgacg atgccgtccg
      541 cgcgctcttc ctcgaaatcg ctcgcggcga cgcctggaat cgcgtcttcg tggccctcga
      601 tttctttgac gcctcgatca accgcatcgc cgcttacgtc gttggcgcgc gcgccacgcg
      661 ccaggcgatc ctgctcggcc ttctcgatcc gtcgaagcaa ctgcgcaccg ccgaatccgc
      721 caaccgcggt cacgaacgcc tagcattgat ggaaacggcg aaagccctcc cgtggggcgc
      781 cgtgtgggac gaactctgcg cccgcgacaa cgttcccgct cgcgccgact ggctcgccgc
      841 cgtcgccact tacgaaaaac aagtcctgtc tcagcgctga gcccagcaat ccgcgagccg
      901 ccaatccgct ctaccgacgc agagtgtaac ctattaggtt acatgctacc tgttgtccgg
      961 ccacggtggc acagtgtgat ggcggccggt tttggtgctg acttagcttc gatcgttaca
     1021 tgcgcgcatc cgctccagcg ccgcctggag ttttttgatg tcgatcggct tcgtcagatg
     1081 ctcctcgaag ccggcggcgc gccctcgcgc gatgtcgtct tccatgccgt agccggagag
     1141 agcgacgccg cgcaaacggt ggcgctcgcg caattgccgc atcaacgcat acccgtcgat
     1201 gtccggaagc ccgatatcgg agatcaccag atcgaacgtg ctcgccgccg cgagccggat
     1261 cgcctccgcc gcgttcgtcg ccgacttcac gcgatagccg cgtcgcccga gcagccgcgt
     1321 caccgccatg cgcgtcggtt cgtgatcctc caccaacaga attctcgttc cctcgccggt
     1381 ttgctccgca ccgttcgtga cgttcgccgg ctcctccacg cgattctccg gcttcggcgg
     1441 atcgacgacc ggcaatcgaa tgcaaaacgt cgcacctcgc ccgcgccccg cgctccgcgc
     1501 cgaaatttct ccgccatgca gctccatcaa catccgcgaa atcgccagcc ccagcccgag
     1561 tccgccaaac cggtgcgtgc tgtgccgcag cgcgtggtcg ccttgcgaga acgatttgaa
     1621 gctccgcccg atctcgtcct ccgtcatgcc gattcccgta tcggaaatgt cgatatgcag
     1681 ttgcgcgttc tccgttcgag acgtcactga gatcgcgccg cgttgtggag tgaatttcac
     1741 cgcgtttttt aacacgttcc aaaacacct
//
LOCUS       DASPLT010001650         1312 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_80773, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001650 DASPLT010000000
VERSION     DASPLT010001650.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1312)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1312)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1312
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_80773"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..435)
                     /locus_tag="VEA63_16780"
     CDS             complement(<1..435)
                     /locus_tag="VEA63_16780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009513029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HYD85727.1"
                     /translation="MKLIDLNRDGGIGANSLFVQFGDLRVLIDCGLNPKKVGRAATPD
                     LTPLRSAPLDLIIITHCHLDHIGSLPVVMREHPNTPVIMTQGSRMLIERMLHNSANVM
                     MRQKEEDNIPDYPLFTHDEIDRCSGRLTGLPMGHVKKFRGARD"
     gene            complement(485..871)
                     /locus_tag="VEA63_16785"
     CDS             complement(485..871)
                     /locus_tag="VEA63_16785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ApaG domain"
                     /protein_id="HYD85728.1"
                     /translation="MSCSSSEVPGLSARLDKLAYHHGGPSLPADKPHAFVYFITIENS
                     SDRTITLLGRKWVVVHADGSQLVVEGDKIVGETPRLVPGEQFSYNSYHVAGCDAVAHG
                     CFHGVDEHGNRIHVLLPPFEMVIPRS"
     gene            1062..>1312
                     /gene="purH"
                     /locus_tag="VEA63_16790"
     CDS             1062..>1312
                     /gene="purH"
                     /locus_tag="VEA63_16790"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009507848.1"
                     /note="involved in de novo purine biosynthesis; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HYD85729.1"
                     /translation="MAKLALLSVSDKRGLVEFASALVAQHGYTLLSTGGTAKLLAERG
                     LPVTEVSQHTGFPEIMEGRVKTLHPKIHGGLLCRRDKAE"
BASE COUNT          267 a          365 c          456 g          224 t
ORIGIN      
        1 gtcgcgcgcg ccgcgaaatt ttttcacgtg tcccatgggc aggccggtga ggcggccgga
       61 gcagcgatcg atctcgtcgt gcgtgaagag gggataatcg ggaatgttgt cctcctcttt
      121 ctggcgcatc atcacattgg ccgaattgtg cagcatgcgt tcgatcagca tgcggctgcc
      181 ctgggtcatg atgacgggcg tattcggatg ctcgcgcatg accacgggca ggctgccgat
      241 gtggtcgagg tgacagtgcg tgatgatgat caggtcgagc ggcgcactgc gaagcggcgt
      301 gagatccggg gtggcggcgc ggccgacttt cttcggattc aggccgcagt cgatgaggac
      361 acggaggtcg ccaaactgga caaaaagtga gttggcgccg atgccgccgt cgcgattcaa
      421 atcaatgagc ttcatgtagc ccgccgacag agaacgagaa aaccggggag aacgagaacg
      481 tttttcaaga ccgcggaatc accatctcga acggcggcag cagaacgtgg atgcgattgc
      541 cgtgctcgtc gacgccgtga aaacagccgt gcgcgacggc atcgcagccg gcgacgtggt
      601 agctgttgta ggagaactgc tcgccgggaa cgagccgcgg cgtctcgccg acgatcttgt
      661 cgccttcgac gacgagctga ctgccatcgg cgtgcacgac gacccatttg cggccgagaa
      721 gcgtgatggt gcggtccgag gaattttcga tcgtgatgaa atacacgaag gcatgcggct
      781 tgtcggcagg cagcgaagga ccgccgtgat gataggcgag tttatcgagt cgcgcgctca
      841 ggccgggaac ttcgctggag gaacaagaca cgccgcaaac agacgcgggt ttgtgcgctc
      901 ggcaagcggg gaaatggccg gtgccgcttg gcaagcggct tcggaggcgg cacggcgccg
      961 cggactcaga gagcacgcct catgcggaaa gagcgatgcg cgaaacgtgc cgagtatcgg
     1021 cttgctgcgt tgcggcgcgc agacacaaag tcgccgcagc catggccaaa ctcgcccttc
     1081 tttcggtcag cgacaaacgc ggtctcgtcg agttcgcgag tgcgctcgtt gcccaacacg
     1141 gatacacgct gctctcgacc ggcggcacgg caaagctgct cgcggagcgc gggctgccgg
     1201 tgacggaggt gagccagcac acgggcttcc cggagatcat ggaagggcgc gtgaaaacgc
     1261 tgcacccgaa aatccatggc ggtttgctgt gtcgacggga caaggccgag ca
//
LOCUS       DASPLT010001651         1370 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_80824, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001651 DASPLT010000000
VERSION     DASPLT010001651.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1370)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1370)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1370
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_80824"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1361)
                     /gene="priA"
                     /locus_tag="VEA63_16795"
     CDS             complement(<1..1361)
                     /gene="priA"
                     /locus_tag="VEA63_16795"
                     /inference="COORDINATES: protein motif:HMM:TIGR00595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HYD85730.1"
                     /translation="MIETMLPAAVRNGATVKHEKSLVVAQTLPAEELAALEKRAPQQA
                     KVYRFLAQQFRSQKKSLVLSRLGVTAAVVNPLVKRGVLREELRRVERVAYGDDFAHGE
                     LVASQPHALNAEQAAAVAALDATIAEDKFGVTLLHGVTGSGKTEVYLRAIDTALKAGG
                     GVIFLVPEVALTPQTVARLRSRLEAISPGNKCVVWHSHLSEGERFDGWLALATGEARI
                     VVGARSAVFAPVQKLRLIVVDEEHEPAYKQDETPRYHGRDVAVMRAKLNGATCLLGSA
                     TPSLESFVNAEAGKYRLLQLLQRVDARQLPHIHVVDLRIEAMRQRTLPSLSRKLVDAM
                     QGRFERKEQTILFINRRGYSSSMLCTKCGHVEECTHCSIAMTYQRADEMLRCHLCGDQ
                     KPAPARCPKCGAPDIRWKGLGTQRVEEAVRRVLPRARIERMDTDAMSKKNRFREVLAQ
                     FRAG"
BASE COUNT          247 a          534 c          360 g          229 t
ORIGIN      
        1 cccgcgcgaa actgcgccaa cacctcgcga aaccggttct tcttcgacat cgcgtccgta
       61 tccattcgct cgatgcgcgc acgcggcagc acccgccgca ccgcttcctc cacgcgctgc
      121 gtccccagac ctttccaacg aatatccggc gcgccgcact tcggacaccg cgccggtgcc
      181 ggcttctggt cgccgcacaa atgacaccgc agcatctcgt ccgcgcgctg atacgtcatc
      241 gcaatgctgc aatgcgtgca ctcctccacg tgtccgcatt tcgtgcacaa catcgaggac
      301 gagtaaccgc ggcggttgat gaacaagatc gtctgctcct tccgctcgaa ccgcccttgc
      361 atcgcgtcga ccagtttccg cgaaagcgat ggcaaggtcc gctgccgcat cgcctcgatg
      421 cgcagatcga ccacatgaat gtgtggcaac tgccgcgcat ccacgcgttg aagcaattgc
      481 agcaaacggt atttccccgc ctccgcattc acgaaactct ccagcgaggg cgtcgccgaa
      541 ccgagcagac acgttgcccc gttcagcttc gcccgcatca ccgccacgtc gcggccgtga
      601 taacgcggcg tctcgtcctg cttgtaagcc ggctcgtgtt cctcatcgac gacgatcaac
      661 cgcaacttct gcaccggcgc gaaaaccgcc gagcgcgcgc cgacgacgat gcgcgcctcg
      721 ccggtcgcga gcgcgagcca cccgtcgaat cgctcgccct cgctgagatg actgtgccac
      781 accacgcact tatttcccgg cgagatcgcc tccagccgcg agcgcagccg cgccacggtc
      841 tgaggcgtga gcgcgacctc cggcacgaga aaaatcacgc cgccgccggc cttcagcgcg
      901 gtgtcgatcg cgcgcagata aacctccgtt ttccccgaac ccgtcacgcc gtgcagcaac
      961 gtcacgccga acttgtcctc cgcgatcgtc gcatcgagcg ccgccaccgc cgccgcctgc
     1021 tccgcgttca acgcatgcgg ctgcgaagcc accaattcgc cgtgcgcaaa gtcgtcgcca
     1081 tacgccacgc gctccacgcg ccgcaactcc tcgcgcaaca cgccgcgctt caccagcgga
     1141 ttcaccaccg ccgcggttac accgagccgc gacagcacga gcgatttttt ctgcgaacga
     1201 aactgctgcg cgaggaaccg gtaaaccttc gcctgctgcg gcgcgcgttt ctccagcgcc
     1261 gccaactcct ccgccggaag cgtctgcgcg acaacaagcg acttctcgtg cttcaccgtc
     1321 gcgccgtttc gcaccgccgc cggcagcatc gtctcaatca cgctgtcgac
//
LOCUS       DASPLT010001652         1297 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_59618, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001652 DASPLT010000000
VERSION     DASPLT010001652.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1297)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1297)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1297
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_59618"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..667
                     /locus_tag="VEA63_16800"
     CDS             <1..667
                     /locus_tag="VEA63_16800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007359376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HYD85731.1"
                     /translation="FHQLAANTRALLDHLGIDRVHLLGHSMGGMVATRFALMFPDNVA
                     TLTLANPLGLEDWKAKGVPYTSIDAALTNEFRQTPEKIRAYQLENYYHGEWRPAYNEW
                     VEPLVKFLRSSDYARMAWNQALTSDMIYTQPVCYEFSNLKMPVLLVIGQLDRTAPGRN
                     LAPADTRGSLGDYPSLGRATADAIPNAQLVELDDLGHLPHIEAFPRFIAPVKEFLGDH
                     AAR"
     gene            859..>1297
                     /locus_tag="VEA63_16805"
     CDS             859..>1297
                     /locus_tag="VEA63_16805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HYD85732.1"
                     /translation="MAQAPSPETSAKLGSAVFAWDEMVAKPTGVGVRRDINDGPTATM
                     NRFECHISTLKAGLPSHPPHQHAQEELIIIKEGTLDVHINGEEHRIGPGSLFFFASND
                     WHAVRNVGDTDATYLVFNFATDATRTAPKEPAAKSASPDKLPSG"
BASE COUNT          243 a          486 c          329 g          239 t
ORIGIN      
        1 ctttcatcaa ctcgccgcca acacccgcgc actcctcgat cacctcggca tcgaccgggt
       61 tcacctgctc gggcattcga tgggtggcat ggtcgccacg cgtttcgcgc tcatgtttcc
      121 ggacaacgtt gccacgctca ccctcgccaa cccgctcggc ctcgaagact ggaaagcgaa
      181 gggcgtgcct tacacgtcga tcgacgccgc cctcaccaat gaatttagac aaacgcccga
      241 aaagatccgc gcgtatcaac tggaaaacta ctaccacggc gagtggcgtc cggcttacaa
      301 cgaatgggtc gagccactcg tgaagtttct gcgctcctcc gactacgcgc gcatggcttg
      361 gaaccaggcg ctcacctccg acatgatcta cacgcagccc gtgtgctacg agttctccaa
      421 cctgaaaatg cccgtgcttc tcgtcatcgg tcagctcgat cgcaccgcgc ctggtcgcaa
      481 tctcgcgcct gccgacaccc gcggctcgct cggggattac ccctcgctcg gtcgcgcgac
      541 cgccgacgcg attcccaatg cacaactcgt cgagttggac gacctcggtc atctgccgca
      601 catcgaagca ttcccgcgct tcatcgctcc ggtgaaagaa ttcctgggcg atcacgccgc
      661 tcgctgagct gtccggcgcg cgtcgactcg ccagctacgg cgtcgcgcag ccctttgctt
      721 cgacgttgcg ccgaggcgcc cgaagcagaa acctttcggc ctcgcccgcg tcagcttacc
      781 cataccccga aggtcaccat gcatcaccta cgttcgctcc tcgcaatcgc cgccgcttcg
      841 ctcctcgcgc cctgccttat ggctcaagct ccttcccctg aaacgtccgc aaaactcggc
      901 tccgccgttt tcgcgtggga cgaaatggtc gccaagccca cgggcgtcgg cgttcgccgc
      961 gacatcaacg acggtcccac cgccacgatg aatcgcttcg agtgccacat cagcacgctg
     1021 aaagccggcc tcccttctca cccgccgcac cagcacgcgc aggaagagct aattatcatc
     1081 aaagaaggca cgctcgacgt gcacatcaac ggcgaggagc accgcatcgg ccctggctcc
     1141 ctctttttct tcgcatcgaa cgactggcat gccgtgcgca acgtcggcga caccgacgcc
     1201 acgtatttgg ttttcaactt cgccaccgat gccacgcgca ccgcgcccaa ggagccggcc
     1261 gcgaaatccg cctcgcccga taaattgccc tccggtg
//
LOCUS       DASPLT010001653         1899 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_80883, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001653 DASPLT010000000
VERSION     DASPLT010001653.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1899)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1899)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1899
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_80883"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(35..751)
                     /locus_tag="VEA63_16810"
     CDS             complement(35..751)
                     /locus_tag="VEA63_16810"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmE family RNA methyltransferase"
                     /protein_id="HYD85733.1"
                     /translation="MNLILFEPAEIDAPLPRRDPRAVHVLDVLRRKTGDDFDAGIVNG
                     PRGKATVVAIDTDSLTLRFVENFLPAPLDPITLIVGLPRPQTARDILRDATTLGASAL
                     HFVITEKGDPNYAASRLWTAGEWRRHVIAGAEQAFDTRIPRVSHGRSLTEALAALPAG
                     GSALALDNYEAPQSLSASRELQLPVALALGPERGWADADRRTLRAHGFMFVHLGSRVL
                     RAESAVVAALTLVRANLNLA"
     gene            complement(860..933)
                     /locus_tag="VEA63_16815"
     tRNA            complement(860..933)
                     /locus_tag="VEA63_16815"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(899..901),aa:Gly,seq:tcc)
     gene            1147..>1899
                     /locus_tag="VEA63_16820"
     CDS             1147..>1899
                     /locus_tag="VEA63_16820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HYD85734.1"
                     /translation="MKAAQALAKQKSLEKKAKNYKLPLGELALFTQQLASLLVAGLPL
                     VQCLEALQDQTEDQCFRIVIRDVRLDISSGNSFSSAVKKFPKAFPNLFISMVEAGEAS
                     GGLAEILGKVASYFEATVKLTKKVKSAMTYPIAVIGLAVALVNVLLIFVIPVFASMFA
                     DFGAQLPAPTQLLIDLSDFMKAYWWAVGLGAYAVFLFIGKYTQTPAGRRQKDHFLVRA
                     PIFGNLIHKIALSRFCRTYATLIRSGVPILRTL"
BASE COUNT          336 a          509 c          666 g          388 t
ORIGIN      
        1 ttcggcgcgc tgctggcgtg tcgtaggatt ttagttacgc cagattcaag ttcgcccgca
       61 cgagcgtgag agcggcgacg acagcggatt cggcgcgcag cacgcgggat ccgaggtgaa
      121 caaacataaa tccgtgcgca cgcaatgtgc gccggtcggc gtcggcccag ccgcgttcgg
      181 ggccgagcgc gagcgccacc ggcagttgca gctcgcgcga ggcgcttagc gattgtggtg
      241 cttcgtaatt gtcgagcgcg agagcactgc cgccggcggg taacgcggcg agtgcttcgg
      301 tgaggctgcg gccgtggctg acgcgcggga tgcgggtatc aaacgcctgt tcggcgcccg
      361 cgatgacgtg gcgacgccat tcgcccgcag tccacaaccg gctagccgcg taattcggat
      421 cgcctttttc ggtaatgacg aaatgcagcg cggaggctcc cagcgtggtg gcatcgcgca
      481 ggatgtcgcg cgcggtttgc gggcgcggaa ggccgacgat cagcgtgatc ggatcgagcg
      541 gcgcggggag gaagttttct acaaatcgaa gcgtgagcga gtcggtgtcg atcgcgacca
      601 cggtggcttt gccgcggggg ccgttgacga tgccggcatc gaaatcgtcg ccggtttttc
      661 ggcgcagcac gtcgagcaca tggacggcgc gcgggtcgcg gcgtggtagc ggcgcgtcga
      721 tttcggcggg ctcgaagagg atgaggttca caagcggatg aagcggagcg atgcggggag
      781 aacgttcgga gccgcgcgaa tccggcgtag agcgcgagtg ctttgtcgtg cgtgcgcgga
      841 cacgtcattc agcgacgtgt ggagcgggtg gacggaatcg aaccgacgat acctctttgg
      901 aagagaggag ttttaccact aaactacacc cgcgaaaagc agggggagtt tcgtccgacg
      961 tcgggacccg tcaagctcgc gttcggcctg agtggttcac cgggttttta ccggttgttg
     1021 tcttaaatcg ggttgccggt gtgcagaggg tccgtttgcc tcttcaacat ggcattactc
     1081 tcttcggcat cctcggctcc gggttcggtt ccccggacgg atgcggccaa aaagagaggc
     1141 ccgagcgtga aggcggcaca ggccctcgcg aagcagaagt cgctcgagaa aaaagcgaag
     1201 aactacaaac tgccgctggg cgaactggcg ctgttcacgc agcagctcgc gtcgttgctc
     1261 gtggcgggcc tgccgttggt gcagtgtctc gaggcgctgc aggatcagac ggaagaccag
     1321 tgtttccgga tcgtgattcg cgatgtgcgc ctggatattt cgtcgggcaa ctcgttctcg
     1381 tcggcggtga agaaatttcc gaaagcgttc ccgaatctgt tcatctcgat ggtggaagcg
     1441 ggcgaggcga gcggcggctt ggcggaaatc ctcggcaagg tggcgagcta cttcgaagcc
     1501 acggtgaaac tcacgaagaa ggtgaagtcg gcgatgactt atccgatcgc ggtcattgga
     1561 ctcgcggtgg cgctggtcaa cgtgttgctg attttcgtca tcccggtgtt cgcgtcgatg
     1621 tttgcggatt ttggcgcgca attgccggca ccgacgcagt tgctgattga tctctcggat
     1681 ttcatgaagg cgtattggtg ggcggtcggg ctcggcgcct acgcggtgtt tctttttatc
     1741 ggaaaataca cgcaaacgcc ggcgggacgg cggcagaagg accacttttt ggtgcgcgcg
     1801 cccatcttcg gtaatctgat ccacaagatc gcgttgtccc gtttttgccg cacttacgcg
     1861 acgttgatcc ggtcgggtgt gccgattttg cggacgctc
//
LOCUS       DASPLT010001654         1082 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_101083, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001654 DASPLT010000000
VERSION     DASPLT010001654.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1082)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1082)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1082
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_101083"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            19..>1082
                     /locus_tag="VEA63_16825"
     CDS             19..>1082
                     /locus_tag="VEA63_16825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD85735.1"
                     /translation="MIPAPTMRFASPLLLLAGFVSLGAALIGLAANAHAGVAPYRKID
                     LNQDFFAEGATFGDLNRDGKADAIAGPFWYEGPDFTRRHELYTPPAPFDPLGYSNNFS
                     AFVRDFNADTWPDVLLIGFPGKDASWLENPGAKGGAWRRHHVYEPVDNESPTFGDLLG
                     DGRPVLICMSLGRIGYATPDAQDPTKPWTFHPASPPGSWHHFTHGLGFGDVNADGRAD
                     LLEKDGWWEQPASLDGNPFWKHHRFTFGSGAHIHVTDVNGDQLPDVITSLNAHGYGLA
                     WFEQTRTADGAIDFRRHDILSSEAEPKINGVQFSQLHAVTLADIDGDGLDDIVTGKRW
                     WAHGPSKDPEPNAAPVIYAFL"
BASE COUNT          190 a          410 c          282 g          200 t
ORIGIN      
        1 gtgctaaact caacttcctt gatccccgcc ccgacgatgc gttttgcttc tccgctgctt
       61 cttctcgccg gctttgtgtc actcggcgcc gccttgatcg gcctcgccgc gaacgcgcat
      121 gcgggcgtgg cgccgtatcg caagatcgac ctcaaccagg atttttttgc cgagggcgct
      181 acgttcggcg acctcaaccg cgacggaaaa gccgacgcga tcgccggccc gttctggtat
      241 gagggccccg acttcacgcg ccgtcacgaa ctctacaccc cgcccgcgcc cttcgatccg
      301 ctcggttact cgaataattt ttccgccttc gttcgcgact tcaacgccga cacgtggccc
      361 gacgttcttc tcatcggctt tcccggcaaa gacgcatcgt ggctcgagaa ccccggcgca
      421 aagggcggcg cctggcgacg tcatcacgtc tacgaacccg tcgacaacga atcgcccacc
      481 ttcggcgatc tgctcggcga cggacgtccc gtgctgattt gcatgagcct cggccgcatc
      541 ggctacgcga cgcccgacgc gcaggatccc acgaagccgt ggacctttca ccccgcgtcg
      601 ccgcccggct cgtggcatca cttcacccac ggcctcggct tcggcgacgt caacgccgac
      661 ggccgtgccg atcttctcga gaaagacggc tggtgggaac aacccgcctc gctcgacggc
      721 aatccgtttt ggaaacatca tcgcttcacc ttcggcagcg gcgcgcacat ccacgtcacc
      781 gacgtcaacg gcgaccaact ccccgacgtc atcacctccc tcaacgccca cggctacggt
      841 ctcgcgtggt tcgaacaaac tcgcaccgcc gacggcgcga tcgatttccg ccgccacgac
      901 atcctttcgt ccgaagccga acccaaaatc aacggcgtgc aattctcgca acttcacgcc
      961 gtcacgctcg ccgacatcga tggcgacggc cttgacgaca tcgtcaccgg caaacgctgg
     1021 tgggcgcatg gcccgtcgaa agatcccgag cccaacgccg cgcccgtcat ctacgcgttt
     1081 ct
//
LOCUS       DASPLT010001655         2145 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_121746, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001655 DASPLT010000000
VERSION     DASPLT010001655.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 2145)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2145)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2145
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_121746"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..909)
                     /locus_tag="VEA63_16830"
     CDS             complement(<1..909)
                     /locus_tag="VEA63_16830"
                     /inference="COORDINATES: protein motif:HMM:NF012722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HYD85736.1"
                     /translation="MRIRTAIFVVYVAASAVGFAVLMRYMLAEVRPRYVNALHFNLEA
                     AARLLAASLDRRPELAVEALREADSGLRVRIVRDDGAVLLDTSDSAETEVEDYSVASS
                     GLAKRAAIGLLDERPLDGATEIGASAPLTFADGRSGTVVLSRPIRTVNAFVWAERKRL
                     ASIGLLIAAVMLIAGWWIAARLTHAIEKLTVYAQAVRDGRRVAPPRSRAREIATLTKA
                     FDEMRDALEGRQHAERYTQALAHEVKAPLAAIRGAAELLDEAMPEEQRRKFMANIRGE
                     SARIEQIVERLLELSSLEARKALRQTE"
     gene            complement(1094..1786)
                     /gene="creB"
                     /locus_tag="VEA63_16835"
     CDS             complement(1094..1786)
                     /gene="creB"
                     /locus_tag="VEA63_16835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009513139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CreB"
                     /protein_id="HYD85737.1"
                     /translation="MRQTILVVEDETAIAETIVYALQTEGFVALWRATGREALAILSE
                     QTVALVVLDIGLPDGNGLDFCREIRRSSKVPVIFLTARSGELDKVVGLELGADDYVAK
                     PFSPRELTARVRAVLRRTNGGDAQSAGSNAGNAAAWAHDEARCRIAYRGRALELTRNE
                     YRLLSVLLAQPGRVFSREQLMAAAWDDPLAALDRTVDAHIKTVRAKVREVSEGADPIV
                     THRGLGYSLREE"
     gene            complement(1960..>2145)
                     /locus_tag="VEA63_16840"
     CDS             complement(1960..>2145)
                     /locus_tag="VEA63_16840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD85738.1"
                     /translation="LFHCGARVLSVVASMMEYDPTRRAVRPVRVRPAMVARVRAAKIL
                     PMPASLRGGLLEQRPAA"
BASE COUNT          412 a          818 c          560 g          355 t
ORIGIN      
        1 ctccgtttgt cgcagcgcct tgcgtgcctc gagcgacgac agctccagca accgttcgac
       61 gatctgctcg atccgcgccg actccccgcg aatgttcgcc atgaacttcc ggcgctgctc
      121 ctccggcatc gcttcgtcca acaactccgc cgcgccccga atcgccgcca gcggcgcctt
      181 cacctcgtgt gccagcgcct gcgtgtaacg ctccgcatgc tgacgcccct cgagcgcgtc
      241 gcgcatttcg tcgaacgcct tcgtcaatgt cgcgatctct ctcgcacgcg accgcggcgg
      301 cgcgactcgc cggccatcgc gcacggcctg cgcataaacc gtgagcttct cgatggcgtg
      361 cgtcagccgc gccgcaatcc accagccggc gatcaacatc accgccgcga tcaacagccc
      421 aatgctcgcg agcctcttcc gctccgccca cacaaacgcg ttcaccgtgc ggatcggccg
      481 cgacagcaca accgtgccgg atcggccgtc ggcaaacgtc aacggcgccg acgcaccaat
      541 ttccgttgcg ccatcaagcg gcctctcgtc gagcaacccg atcgccgcgc gtttggccaa
      601 gccgctgctc gcaaccgaat agtcctccac ctcggtctcc gcagagtccg acgtgtcgag
      661 cagcacggcg ccgtcatcgc gcacgatccg cacgcgtagt ccggaatccg cttcgcgcag
      721 cgcctccacc gccaactccg gacgccgatc cagtgatgcc gccaacaacc gtgccgcggc
      781 ctcgagattg aagtgcagcg cgttcacata ccgcggacgc acttccgcca gcatgtagcg
      841 catcaacacc gcgaagccca ccgcactcgc ggcgacatac acgacgaaga tggccgtgcg
      901 aatcctcatc cggaacaatt catttttaac cacgaatgga cacgaatcga cacgaatgaa
      961 accgagttcg aatgcgtcat cttttgggaa caccaatggg tgatgtcacg atcggccctg
     1021 aaagcttcct tctctcctct gaattcgtgt tcattcgtgt ccattcgtgg tttcgcttct
     1081 gcacgagtcc ggctcattcc tcgcgcaacg agtagccgag cccgcgatgc gtgacgatcg
     1141 ggtccgcgcc ctcgctcacc tcgcgcactt tcgcccgcac ggttttgatg tgcgcgtcca
     1201 ccgtccgatc gagcgccgcg agcggatcgt cccacgccgc cgccatcaat tgctcgcggc
     1261 tgaacacgcg ccccggttgc gccagcagca cgctcaacag acgatactcg ttgcgcgtca
     1321 gctcgagcgc ccgcccgcga taagcgatcc ggcagcgcgc ctcgtcatgc gcccaggccg
     1381 cagcgtttcc cgcgttcgat cccgccgatt gcgcatcccc gccgttcgtc cgccgcaaaa
     1441 ccgcccgcac ccgcgccgtc agttcgcgcg ggctgaacgg ctttgcgacg tagtcgtccg
     1501 ccccgagctc caagcccacc accttgtcga gttcgccgct gcgcgccgtg agaaaaatca
     1561 ccggcacctt cgaagaacgc cggatctcgc ggcaaaaatc cagaccgttt ccatccggca
     1621 gtccgatatc gagcaccacc aacgccaccg tctgctcact caaaatcgcg agcgcctcgc
     1681 gcccggtcgc gcgccaaagc gcgacgaaac cctcggtttg cagcgcatac acgatcgtct
     1741 ccgcaatcgc ggtctcgtct tcgacaacga ggatcgtctg acgcaagggt gacatccggc
     1801 tcgttcatga gagttccccg gcgcgtttca agattctcaa caactcgcac gacaaacacc
     1861 tcgcgcgttc gaaccaaaaa aaaagcccgc accgaagtgc gggctcatca aatcgaatca
     1921 gttcagcgca gaacgaccgg ttcagcgcga acttgaactt cacgccgccg gacgctgctc
     1981 cagcaaaccg ccgcgcagcg acgccggcat cggcagaatc ttcgccgcgc gcacccgcgc
     2041 caccatcgca ggacgcaccc gcaccggccg caccgcgcga cgcgtcggat catattccat
     2101 catcgaagcg acgacactca acacgcgcgc gccacagtga aacag
//
LOCUS       DASPLT010001656         1846 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_244383, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001656 DASPLT010000000
VERSION     DASPLT010001656.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1846)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1846)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1846
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_244383"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..266
                     /locus_tag="VEA63_16845"
     CDS             <1..266
                     /locus_tag="VEA63_16845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD85739.1"
                     /translation="SFEKGTPGENGGPYALKLKNTSDSALTVSATIIWSVASHNRAKT
                     INLPPHEIAAGESWTIDDLAAEDRVILSADNFEKLEVKVPPGK"
     gene            503..1408
                     /gene="rlmF"
                     /locus_tag="VEA63_16850"
     CDS             503..1408
                     /gene="rlmF"
                     /locus_tag="VEA63_16850"
                     /EC_number="2.1.1.181"
                     /inference="COORDINATES: protein motif:HMM:NF017755.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008988 - rRNA
                     (adenine-N6-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(1618)-N(6))-methyltransferase
                     RlmF"
                     /protein_id="HYD85740.1"
                     /translation="MHPRNRFAGGYDFSRLVRRCPELARFVHANPTGIATIDFADPAA
                     VTLLNRALLLDAYGLAHWDIPVGQLCPPIPGRADYLHHIADLLADGQTAPPRGPAIAL
                     LDIGVGASCIYPIIGVAEYDWRFVGTDIDPASLAWARKLIAANPTLSGRIECRFQPLR
                     SNIFRGAVKSDETFAASICNPPFHASAEDAAAGTLRKLKNLNRGKATSPVRNFGGRAN
                     ELWCEGGEAAFVARMIAESAQRPELCRWFTTLVSKRETLPAIHRALQRVKATEVRVID
                     LAHGQKKSRIVAWRFAPGPYGRARA"
     gene            complement(1598..>1846)
                     /locus_tag="VEA63_16855"
     CDS             complement(1598..>1846)
                     /locus_tag="VEA63_16855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD85741.1"
                     /translation="VCRRVDWPMQPDNTTPSRTGTGTKNDKSGKTVGRQDIPKEHREL
                     EGVDEGPTEGALPPTSTEPNTTGLRSGPRRNVRTGLHS"
BASE COUNT          332 a          654 c          502 g          358 t
ORIGIN      
        1 cgtccttcga aaaaggcact cccggcgaga acggcgggcc ctacgccctg aagctgaaaa
       61 acacgtctga ctctgcgctc accgtcagcg ccaccatcat ctggagcgtc gcctctcaca
      121 accgcgctaa aaccatcaat ctcccgccgc acgaaatcgc cgccggcgaa agttggacga
      181 ttgacgacct cgctgccgag gaccgagtca tcttgagtgc cgacaatttc gaaaagctgg
      241 aagtcaaagt cccgcccggc aaatgacggc taaagtagaa gtgtagccga gatctactgt
      301 gggatgggct ttatgccccg actggttggt gcccgttcga cgggacacaa agccctcccc
      361 acagcctcga atcacactgg caacgacatc gaccgcggct gcagtatttc cagatgcgcg
      421 ctaacacggt tgtcatcgta atcgcacgtt ccgactgaac tcctcttcaa gagcttcgaa
      481 tacggcgcca ccgaaaagcg gtttgcatcc gcgaaaccgc ttcgccggcg gctacgattt
      541 ttcgcgcctg gtccgccgtt gcccggagct cgctcgcttc gttcacgcga atcccaccgg
      601 catcgcgaca atcgacttcg ccgatcccgc cgccgtcacg cttctcaacc gcgcgctgtt
      661 gctcgatgcc tacggcctcg cgcattggga cattcccgtt ggtcagcttt gtccgcccat
      721 tcccggacgc gccgactacc tccaccacat cgccgatctg ctcgccgacg gtcaaaccgc
      781 tccgcctcgg gggcccgcaa tcgcgctgct cgatattggc gtcggcgcga gctgcattta
      841 tccgatcatc ggcgtggccg aatacgactg gcgcttcgtc ggcaccgaca tcgatcccgc
      901 ctcgctcgcg tgggctcgca aactcatcgc cgccaatccc acgctctccg gccgcatcga
      961 gtgtcgcttc caacccctcc gctccaacat tttccgcggt gctgtgaaat ccgacgaaac
     1021 cttcgcggcg tcgatctgca atccaccctt ccacgcctcc gcggaagacg ccgccgccgg
     1081 cacgttgcga aagttgaaaa atctcaaccg tggaaaggcg acctcaccgg tccgaaattt
     1141 cggcggacgc gccaacgagc tctggtgcga aggcggagaa gccgcgttcg ttgcccgcat
     1201 gatcgccgaa agcgctcaac gccccgaact ttgccgctgg ttcacgacgt tggtctccaa
     1261 gcgtgaaacc cttccagcga tccatcgcgc cttgcagcgc gtgaaggcca ccgaagttcg
     1321 cgtgatcgat ctcgcgcacg gccagaagaa aagtcgcatc gtcgcctggc gcttcgcccc
     1381 cgggccatac ggccgcgcgc gggcctaagt ttccgtagcc agtggcagca ggcctccgtg
     1441 cctgcctgct tctgcaggtt gcgtgctcgc gcgcaacacg tggcccgacg agcacctgaa
     1501 ttccgctctc agccacagac cagccgcgac cttcagcggc gacggttttg ctaatccgct
     1561 cgaagcgaat ccacgctcgc gcgtccgcct gcagacgtca cgaatgcagc cccgtgcgca
     1621 cgttgcgacg cggtccgctc cgcaagcccg tcgtgttcgg ttccgtcgag gtcggcggaa
     1681 gtgcaccctc ggtcggccct tcatccacgc cttcgagctc gcgatgttct ttggggatgt
     1741 cctgtcgtcc caccgttttt ccggacttgt cgtttttagt gccggtgccg gtccggctcg
     1801 gagtcgtatt gtcgggttgc atcggccaat ctactcgccg gcatac
//
LOCUS       DASPLT010001657         2596 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_121955, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001657 DASPLT010000000
VERSION     DASPLT010001657.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 2596)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2596)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2596
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_121955"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..791
                     /locus_tag="VEA63_16860"
     CDS             <1..791
                     /locus_tag="VEA63_16860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HYD85742.1"
                     /translation="PFQIWIPDVYQGAPTPVTALLAVSSKAAGFAMLLPLTTTVFASY
                     GWLVTPVLTAIAAATILFGNIAALTQHNVKRLIGLSGVSHAGFLLLGVVAAPLAPSAI
                     GAILFYLFAYLLASFVVFGVMVHLAGPNDADQNLDHYTGLAKESPFLATVLAVALGSL
                     AGIPPLAGFMGKFFVFIAAFQAELYGLLAIAIIGVVISIYYYFGWIKAAFFETWTPAD
                     GGVNPRPARTPVGYAAGISLATLALFTVIFGLYQGPLGAWLLAR"
     gene            1000..1623
                     /gene="rrtA"
                     /locus_tag="VEA63_16865"
     CDS             1000..1623
                     /gene="rrtA"
                     /locus_tag="VEA63_16865"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhombosortase"
                     /protein_id="HYD85743.1"
                     /translation="MVTLLRKLFAPQKLPCAFLAIALVVLVIQLNGQWRSALVFDRVA
                     LDAGEWWRIWTGHLVHFGWPHFIADAGLFLILGWLLEREYPIATRIALIAMPAFISGA
                     IYLFDPTMLRFGGLSALNLGLLVFLAAKGWQKNWTDWFWPAVLAIYVGEIVLQSTQRQ
                     GSGGGMIPFDDPAIQVATFAHIAGAVYGGGLALVLILHSRRVVARDE"
     gene            complement(1642..>2596)
                     /locus_tag="VEA63_16870"
     CDS             complement(1642..>2596)
                     /locus_tag="VEA63_16870"
                     /inference="COORDINATES: protein motif:HMM:NF025295.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="immunoglobulin domain-containing protein"
                     /protein_id="HYD85744.1"
                     /translation="DGLGYLYIADTLNHTLRKIDLTTSEVSTVAGQPGTIGSADGRAT
                     EARFYYPSGIAVGSSGSVYVTDSGNNAIREVRIGGSVSTIAGLAGSTGAADGVGVDAR
                     FNNPGGLTRDGAGSTLYVVDTDNHTLRKVNIITGAVSTIAGSAGAFGSADGLGGTARF
                     DFPAAIAWWNNGTTIGIADTANHTVRLGAPASPVTILTQPQSQAQREGTNVSFTVVAS
                     GQPSPTYQWYLNGEAIPGATGNTYSITSLERFRHVGAYTVVVSNAFGNVTSDTATLTV
                     TDGASLGTGGAAPSGWFYLALAAVAAARAWTMRKGSKSARS"
BASE COUNT          394 a          836 c          799 g          567 t
ORIGIN      
        1 ttccgtttca aatttggatt cccgacgtgt atcaaggcgc gccgaccccc gtcaccgcgc
       61 tcctcgcggt ttcgtcgaag gccgccgggt tcgcgatgct gctgccgctc acgacgaccg
      121 tgttcgcatc ctacggttgg ctcgtcacgc ccgtgctcac cgcgatcgcg gccgccacga
      181 ttctgttcgg caacatcgcc gcgctcacgc agcacaacgt gaaacgcctc atcggtctct
      241 ccggcgtttc gcacgccggc ttcctcctgc ttggcgtcgt cgccgcgccg ctcgcaccgt
      301 ccgcgatcgg cgcgatcctg ttttatctct tcgcttacct gctcgcgtcg ttcgtcgtct
      361 tcggcgtgat ggtgcacctc gccggtccca acgacgctga tcagaacctc gatcattaca
      421 ccggcctcgc gaaggagagc ccgtttctcg cgacggtgct cgcggtcgcg ctcggctcgc
      481 tcgccggcat tccgccgctc gccggtttca tgggaaagtt cttcgtgttc atcgccgcgt
      541 ttcaggcgga gctctacggt ttgctcgcga ttgcgatcat cggcgtggtg atttcgatct
      601 actattactt cggctggatc aaagcggcgt tcttcgaaac gtggacgccc gccgacggcg
      661 gcgtgaaccc gcgccccgcg cgcacgccgg tcggctacgc cgccggcatc tcgctcgcca
      721 cgctagccct tttcacggtg atcttcggcc tctaccaagg cccccttggc gcctggctgc
      781 tggcgcggtg atttgcacgg ccgctctgca accttgtcgg gcaggtcacc cgaccgccgg
      841 tggttgacgc aggcacaagg cgcaagacgg cgggatcggg agatcccgcc ctacatccct
      901 gctgtagggc gacgccgccc accttcggca tcgtttcctt tttctggcgc ttccaatccg
      961 acgcgtgcgt attggttcgg ttgagtcttt ccgaccccaa tggtcactct gcttcgaaaa
     1021 ctcttcgcac cccaaaaact gccatgtgcg tttctcgcga tcgcgcttgt cgttctcgtt
     1081 atccaactca acggccagtg gcgcagtgcg ctggtttttg accgtgtcgc actcgacgcg
     1141 ggcgaatggt ggcgaatttg gacgggacac ttggtgcact tcggctggcc gcatttcatc
     1201 gccgacgcgg gcctgttctt gatcctcggc tggctgctcg aacgcgagta tccgatcgcc
     1261 acgcggatcg cgctgattgc gatgccggcg ttcatcagcg gagcgattta cctgttcgat
     1321 ccaacgatgc tccggtttgg cgggttgagt gcgctcaacc tcggcctcct ggtttttctc
     1381 gcagccaaag gctggcagaa gaattggacc gattggtttt ggccggcggt gctcgcgatc
     1441 tacgtcggcg aaatcgtgct gcaatccacg cagcgccagg gctccggcgg cggcatgatt
     1501 ccgttcgacg atcctgcaat tcaagtggcc accttcgcgc acatcgcggg cgcggtttat
     1561 ggcggcggac tcgcgctggt cctgattttg cacagccgcc gtgttgttgc ccgcgatgag
     1621 tgaggggtgg agcgcttctg tttaggagcg cgcgcttttg gagccctttc gcatcgtcca
     1681 cgctcgtgcg gctgcgaccg cggcgagggc gaggtaaaac catccgctcg gcgctgcgcc
     1741 gccggtccca agagaagctc cgtcggtgac cgtcagtgtt gcagtgtcgc tggtgacgtt
     1801 cccaaaggcg ttgctaacga caaccgtgta ggcaccgaca tgtctgaagc gctccaggct
     1861 ggttatgctg taggtattgc ccgtggctcc aggaatggcc tcgccgttca aataccattg
     1921 atacgtgggc gagggctgac cgctggcaac aacggtgaaa ctgacattcg tgccctcgcg
     1981 ctgcgcctgg ctttgtggtt gcgtgaggat ggtgacagga gacgccggcg cgccgagccg
     2041 gacggtgtga ttcgcggtgt cggcgatgcc gattgtggtg ccgttattcc accaggcgat
     2101 ggccgccgga aaatcgaaac gcgccgtccc gcctagacca tcggcgctgc caaacgcgcc
     2161 tgccgaaccg gcgatggtcg agactgcgcc cgtgatgatg ttaactttgc ggagggtgtg
     2221 attgtcggtg tcgacgacgt agagagtcga accagcgcca tccctcgtga ggccgccggg
     2281 attgttgaaa cgtgcatcga cgccaacgcc gtccgccgca ccggtgctcc cggccaaacc
     2341 cgcgatcgtg ctgacggagc cgccaattct gacttcacga atcgcgttat tcccgctgtc
     2401 ggtgacgtaa accgagccgc ttgaaccaac cgcaatgccg gacggatagt agaaccgggc
     2461 ttccgtcgct cgtccatcgg cgctgccgat cgtgccgggc tggccagcca cggtgctgac
     2521 ttcgctcgtc gtcaggtcaa ttttgcgaag ggtgtggttg agcgtgtccg cgatgtagag
     2581 atagcccaag ccatcg
//
LOCUS       DASPLT010001658         1442 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_59861, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001658 DASPLT010000000
VERSION     DASPLT010001658.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1442)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1442)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1442
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_59861"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..709)
                     /locus_tag="VEA63_16875"
     CDS             complement(<1..709)
                     /locus_tag="VEA63_16875"
                     /inference="COORDINATES: protein motif:HMM:NF013117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel domain-containing
                     protein"
                     /protein_id="HYD85745.1"
                     /translation="MRWKPLFDFFGDPVLAIVAVALGAALAALLVQIIATKIITRLIS
                     HHLYLRTFLKRSIPPMRLLLPLLAVQSVLQTLRPDRFEWVNGVQHGLLVLIVLTVTWL
                     AVEAVLGMRDVAMLRFPVDRPDNLDARRILTQATVLTRVTSGVIMLIGVAIALMTFPG
                     VRQIGASLLASAGLAGIVVGFAARPVLGNLIAGLQIAMSQPIRIDDVVIVEGEWGRIE
                     EITGAYVVVKIWDERRLV"
     gene            961..1215
                     /locus_tag="VEA63_16880"
     CDS             961..1215
                     /locus_tag="VEA63_16880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD85746.1"
                     /translation="MKTGKQLPKYEHLKTTEQPGDHPMQEKNQQREGAQTDIPGEMHQ
                     GRGESVPANERPKAKNTDDEPTVDMMKPTFPLKDEKGRSE"
BASE COUNT          317 a          435 c          482 g          208 t
ORIGIN      
        1 cgacgagtcg gcgctcatcc cagatcttca cgacgacgta agcgccggta atctcctcga
       61 tgcggcccca ttccccttcg acgatgacga cgtcgtcgat gcggatgggt tgcgacatcg
      121 cgatctgcaa gccggcgatg aggttgccca gcaccggccg tgctgcgaaa ccgacgacga
      181 taccggcgag gccggcggag gcgagcaggc tcgccccgat ttggcgcaca ccggggaagg
      241 tcatgagcgc gatcgcgaca ccgatcagca tgatcacgcc ggaggtcacg cgcgtgagca
      301 ccgtggcttg cgtgaggatg cgtcgcgcat cgaggttgtc cgggcgatcg acaggaaagc
      361 gcaacatcgc cacatcgcgc atgccgagca ccgcttcgac ggcgagccag gtgaccgtca
      421 gcacgatcaa gacgagcagt ccatgctgca cgccgttgac ccattcgaag cggtcgggac
      481 gcagcgtctg caggaccgac tgcaccgcga gcagcggcag cagcaggcgc attggtggga
      541 tggaccgctt gaggaaggtg cggagataaa gatggtgcga gatgagccgg gtgatgatct
      601 tcgtggcgat gatctgcacc agcagagcgg ccaatgcggc gccgagcgcc acggcgacga
      661 ttgcgaggac gggatcacca aaaaaatcga atagcggttt ccagcgcatg ggcgttttcc
      721 gtggttgttg gttgatttat cgcccggtgt gcgggcggcg ggcgcgcggg gagcgcggct
      781 ttgttgtgcg attcgagcaa gtacgacgcc gggttcgcgc agaagttgcg cggatcgctc
      841 gatattccgg catgcgaaaa gacgcctcgc ggtcgcacga gcgagcaggc atagggcctg
      901 cacccggagt gtagcgcgcg cgacgcgcgc gcctaaacgt tatctcgcga ggacattgcg
      961 atgaaaaccg gcaagcaact cccgaaatac gagcatctca agaccacgga acagccgggc
     1021 gatcacccga tgcaggaaaa gaatcagcag cgcgaaggcg cgcaaaccga tatcccgggt
     1081 gagatgcatc aagggcgggg cgaatcggtt ccggcgaacg aacgtccgaa agccaagaac
     1141 acggatgacg agccgaccgt cgacatgatg aagccgacgt ttccgctgaa agacgagaaa
     1201 ggccgatccg aatagcgcgg atcacgcgcc attcatggcc ggcctgcggc ggcgtcgcga
     1261 acgaagaacg ctcacttcga cgccgccatg gtcaccactg caccggcgac caccaatccg
     1321 acgcccagca cgcgcagcgg cgagatcacc tgttgcgtca gcccgagcac gccgaaatga
     1381 tccatcagca atgaggacgt catctggccc gcgacgatga tggcgagcat cgccgcggcg
     1441 cc
//
LOCUS       DASPLT010001659         1647 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_244673, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001659 DASPLT010000000
VERSION     DASPLT010001659.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1647)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1647)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1647
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_244673"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(134..820)
                     /locus_tag="VEA63_16885"
     CDS             complement(134..820)
                     /locus_tag="VEA63_16885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD85747.1"
                     /translation="MRPPRPPRRRFVLLLLLASMLVNAACGASGSSSSLALGLSLSCP
                     APELIRDDDAFFSFARELSRDVDRLLADPPATSPKHKRLLALRVHLALCLRDDAVARA
                     AAEHLPDLQTDPGERAHSGVLTRALIDSQRDPDAIERVLLAALQALPRDPAVRSAVQR
                     SAAKIAALSEASLVAEIRDDVAPRLARGEPLTLEIADQIVRVGHRLRNILPLRAALLR
                     AYDKALTTWP"
     gene            1201..>1647
                     /locus_tag="VEA63_16890"
     CDS             1201..>1647
                     /locus_tag="VEA63_16890"
                     /inference="COORDINATES: protein motif:HMM:NF013431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-epimerase"
                     /protein_id="HYD85748.1"
                     /translation="MTPVRTALGLIALLVVSLRSAPAAIRVANAGLMPDGSEVKSFTL
                     ENDRGVRATLLEYGAILASLEVPDRNGKLADITLGYDSLDGWLSNTSYFGAVVGRHAN
                     RIAAGRFTLDGKSYTLATNNAPGGMPCHLHGGNVGFDKRHWRGEIVE"
BASE COUNT          330 a          461 c          587 g          269 t
ORIGIN      
        1 ttggttcatg ggcaaatacg atgaaaagag gggagagaac aaaggctgtg cagcggttga
       61 tcgcggccaa cggttcgaac taggtccgga ttcaatacca gtgcgggtgc ggaagggcgg
      121 gagttgagcg gcgtcacggc cacgttgtga gcgccttgtc gtaggcgcgc agcagggcgg
      181 cgcgaagcgg caggatgttg cgcaaacgat gtccgacgcg gacgatctgg tctgcgattt
      241 ccaacgtgag cggttcgccg cgcgccaatc gtggggcgac gtcgtcgcga atttccgcga
      301 ccaatgatgc ttcggaaagc gcggcgattt tggcggccga tcgctgcacc gcgctgcgca
      361 cggcggggtc gcgcggcaga gcttgtaatg ccgcgagcag cacgcgttcg atggcgtcgg
      421 ggtcgcgttg agagtcgatc agcgcgcgcg tgaggacgcc ggaatgcgcg cgctcgcccg
      481 gatcggtttg caagtcgggc agatgttcgg cggcggcgcg cgcgacggcg tcgtcgcgca
      541 ggcaaagcgc gagatgcacg cgcagcgcga ggaggcgttt gtgcttgggg gacgtggcag
      601 gtggatccgc gagcaagcgg tccacgtcgc ggctgagttc gcgagcaaac gagaagaacg
      661 cgtcatcatc ccggatcaac tcgggcgcgg gacaggaaag cgacagaccg agcgcgaggc
      721 tcgagctcga accggacgcg ccgcaagccg cgttcacgag catcgaggca aggagcagca
      781 acaggacaaa ccgacgacgc ggcgggcggg gtgggcgcat gtcatgaatg ggaatcgctc
      841 aagagccgga tgcaatgtcg cgacttaggt ctgacgaacg aaccagcggg ggcggcgagg
      901 ggcagcaaag gtctccgcat catgttgctg ctttcagtcg acggcgtgaa gcagtcgcgt
      961 gcggagcgta tttccacgac gagggcgaag tagcgaggga ccgcgtgctc cacacgcttg
     1021 ggctcgcctg aaaaggattg gcggcggcgg tttacacgca gttgatggct gtcgcgggcg
     1081 agatcaacgg gctcgtaact gacgatcgag agtgacaaag attccgacgg atcgtcttgg
     1141 gcgcttgcat cgggaactga tcgaagccgg aaaaggagag agccaacaat gaaactccaa
     1201 atgacccctg tcagaacggc gctcgggttg atcgccttgc tcgttgtgag tctgcgctcc
     1261 gcgccggccg ccattcgcgt tgcgaacgcc gggttgatgc ccgacggttc cgaagtgaaa
     1321 tcattcaccc ttgaaaacga tcgcggcgtg cgtgcaacgc tgctcgaata cggcgccatc
     1381 ctcgcatcgc tcgaggtgcc cgatcgcaac ggaaagctcg ccgatatcac gctgggctac
     1441 gacagcctcg atggctggtt gtccaacacg tcgtattttg gggccgtggt tgggcgccat
     1501 gccaaccgca tcgccgccgg caggttcacg ctcgatggca aatcctacac gctcgccacg
     1561 aacaacgcgc cgggcggcat gccgtgtcat ctgcacggcg gcaacgtggg cttcgacaaa
     1621 cgccactggc gcggtgagat cgttgaa
//
LOCUS       DASPLT010001660         1594 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_285648, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001660 DASPLT010000000
VERSION     DASPLT010001660.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1594)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1594)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1594
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_285648"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..707)
                     /locus_tag="VEA63_16895"
     CDS             complement(<1..707)
                     /locus_tag="VEA63_16895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD85749.1"
                     /translation="MLRAGPPPPKVVLLPDAMFFTRAVPIAAGSSPASAAGQVELALE
                     TLSPFPPPQLYHGFYWEPGAERALVFAAYRRRFAADQTADWENAELVIPAFASLLGGD
                     VQSDTTVIVPSEEGLTAIVWENGPVPAKVVFRPLAADASDEDRARARDELLGATPRNR
                     SIVLTASPNVETSRREREIAFRAEAFSSRLSAERAGAMDVRDKDALAALRNARRRDLV
                     MWRAFLACVAALIILGLG"
     gene            complement(725..>1594)
                     /locus_tag="VEA63_16900"
     CDS             complement(725..>1594)
                     /locus_tag="VEA63_16900"
                     /inference="COORDINATES: protein motif:HMM:NF045433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD85750.1"
                     /translation="LGFAGWSPREGCTAEVTFRDESAKLSLPKVESAALLNLFEAWGL
                     ARSDAERLTDALFNWMRKDHVPASAWSADYERGAIPYAPPLRSLRSYSELAAIDVARE
                     LFYDEAGRPNELWQRFVDAFSLLDFKQSNLNGASADVLAAMGVSDPMQQKQITDFLQG
                     AGSRERLGPAFFENPAEAAGLIGAGTLPPTAGTTISALRINVLIREGRSTFQLSAVVA
                     PSEGGATLVPPSTLNETKSEEQAATTTPPPAGNKTTKEETPKKLNYPFTLLEIRENAE
                     IPAAPPETEPTQA"
BASE COUNT          282 a          550 c          490 g          272 t
ORIGIN      
        1 cccagcccca aaatgatcag cgcggcaacg cacgcgagaa acgcccgcca catgacgagg
       61 tcgcgccgcc gggcgttgcg caacgccgcg agcgcgtcct tgtcgcgaac atccatcgcg
      121 ccggcgcgct ccgccgataa ccgcgatgaa aacgcttccg cgcgaaacgc gatctcgcgc
      181 tcacgccggc tggtctcgac gttcggcgac gccgtcagca cgatgctgcg gttgcgcggc
      241 gtcgcaccga gcagttcgtc gcgcgcccgc gcgcggtcct cgtccgacgc gtcggcggcg
      301 agcggacgaa aaacaacttt cgccggcacg ggtccgtttt cccaaacgat cgcggtcagt
      361 ccttcctccg acggcacgat caccgtcgta tccgactgga cgtcgccgcc gagcaggctt
      421 gcgaacgcgg gtatgaccag ctccgcgttt tcccagtccg ccgtttgatc cgccgcgaag
      481 cgccgccgat acgcggcgaa cacgagcgcg cgttcggcgc ccggctccca ataaaacccg
      541 tgatagagct gcggcggcgg aaaaggcgaa agcgtctcga gcgcgagctc gacctgaccg
      601 gcggcgctcg cgggcgacga acctgccgcg atcggcactg cacgcgtgaa aaacatcgcg
      661 tcgggcaaca acaccacttt cgggggcggc ggtccggccc ggagcatttt gatcagcggt
      721 aaggtcatgc ctgcgtcggt tccgtttcgg gaggagctgc tggaatctca gcattctccc
      781 taatttccaa gagcgtaaat gggtagttca gttttttcgg agtttcctcc ttggtcgtct
      841 tgttcccggc cggcggcggc gtagtcgtgg cggcttgttc ctctgacttc gtttcgttaa
      901 gcgtcgacgg cggcacgagc gtcgccccgc cttccgacgg cgcgacgacc gcagagagtt
      961 gaaacgtcga acgcccttcg cgaatcagca cgttgatccg caacgcgctg atcgtcgtgc
     1021 cggccgtcgg cggcaacgtg cccgcaccga tcaagccagc cgcctccgcg gggttttcga
     1081 aaaacgccgg cccgagccgc tcgcgactgc cggcgccctg caggaaatcg gtgatctgtt
     1141 tttgctgcat cgggtcgctt acacccatcg ccgcgagcac gtcggcactc gcgccgttca
     1201 agttgctttg cttgaaatcg agcagcgaga acgcgtcgac gaaacgctgc cacaactcgt
     1261 tcggccggcc ggcttcgtcg tagaacagct cgcgcgccac atcgatcgcc gccagttccg
     1321 agtagctccg caacgagcgc aacggcggcg catagggaat cgctccgcgt tcgtagtcag
     1381 cgctccatgc ggaagcaggc acgtggtcct tgcgcatcca attgaacaac gcatcggtca
     1441 atcgctcggc gtcggagcgc gcgagccccc aagcttcgaa gagattgagc agcgcggcgc
     1501 tctcgacctt cggcagcgag agtttcgcac tttcgtcgcg aaacgtcacc tccgccgtgc
     1561 aaccttcccg cggcgaccaa cccgcaaaac cgag
//
LOCUS       DASPLT010001661         1713 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_101341, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001661 DASPLT010000000
VERSION     DASPLT010001661.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1713)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1713)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1713
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_101341"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1348
                     /locus_tag="VEA63_16905"
     CDS             <1..1348
                     /locus_tag="VEA63_16905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD85751.1"
                     /translation="CVSITHASNVGRRFPSEKQTYTDKVTGLLVTVLTADAANDAKPY
                     QTHTTWTADGQWIVFRSNRGDNGSQAFVVNESTGDIVQLTDGPGTGTGSLNLARKSMK
                     LYFMRTLADAASANASSPSSPSTAPATSNASRQLIELDLAKLLPDALAGTVNPDASTY
                     ERVVCTLPTDLRDSGGFALDADETCAYWGVAFGPPPPRPAPTHANRTADGRSVVDSRN
                     TDPTEQREAARTRFAEAGKGPGGIRKIDIATGKITTVIDVDLRMGHVQTNPDVPGEII
                     YCHETTGDAPQRMWTVRGDGTGNRPLYVETADEWVTHETVSGRDEVMFNLMGHLPYLR
                     ERPTGVAVINLRTNTMKLLGQMEEEMANGAQGGFWHCNGSPDQRWAVADNFSGNITLI
                     DRRDSRQRLLTTDHKMKPDHTHPIFSPDGKRVLIQSGHLTDGKSLDLMTVEIPADW"
BASE COUNT          357 a          659 c          420 g          277 t
ORIGIN      
        1 ctgcgtttcc ataacccacg cctccaacgt cggtcgtcgc ttcccttccg aaaaacaaac
       61 ctacaccgac aaggtcaccg gcctcctcgt cacggttctc accgccgatg ccgcgaacga
      121 cgccaaaccc tatcagacgc acaccacctg gactgccgac ggacagtgga tcgtcttccg
      181 ctccaatcgc ggtgacaacg gctcccaagc tttcgtcgtc aacgaatcca ccggcgacat
      241 cgttcaactc accgatggcc cgggcaccgg caccggcagc ctcaacctcg cgcgaaaatc
      301 catgaagctc tacttcatgc gcacgctcgc cgacgcggct tccgccaacg cgtcctcgcc
      361 ctcttctccg tcaaccgcac ccgcgacctc caacgcctcg cgccagctca tcgagctcga
      421 cctcgccaaa cttctccccg acgccttggc cggcaccgtc aaccccgacg cttccaccta
      481 cgagcgcgtc gtctgcactc ttcccaccga cctccgtgac tccggcggct tcgccctcga
      541 tgccgacgag acctgcgcct attggggcgt cgccttcggc ccgccaccgc cacgccccgc
      601 gcccacgcac gccaatcgca ccgccgatgg ccgctcggtc gttgattctc gtaacaccga
      661 tcccaccgaa caacgcgaag ccgcccgcac ccgtttcgcc gaagccggca aaggtcccgg
      721 cggtatccga aaaatcgata tcgccaccgg caagatcacc accgtcatcg acgtcgacct
      781 ccgcatgggt cacgtccaaa ccaaccccga cgtccccggc gaaatcatct actgccacga
      841 gaccactggc gacgccccgc aacgcatgtg gaccgttcgc ggcgacggca ccggcaaccg
      901 cccgctctac gtcgaaaccg ccgacgaatg ggtcacccac gaaaccgtct ccggccgcga
      961 cgaagttatg ttcaatctca tgggccacct gccgtatctc cgcgaacgcc ccaccggcgt
     1021 cgccgtgatc aaccttcgca cgaacaccat gaagctcctc ggccagatgg aagaagagat
     1081 ggccaacggc gcccaaggcg gcttctggca ctgcaacggt tcgcccgacc aacgctgggc
     1141 ggtcgccgac aatttttcgg gcaacatcac gctgatcgat cgccgcgact cgcgccagcg
     1201 cctgctcacc acggatcaca agatgaagcc cgaccacacg cacccgatct tcagccccga
     1261 cggcaaacgc gtgctcatcc aatccggcca tctcaccgac ggcaaaagcc tggacctcat
     1321 gaccgtcgag atccccgccg actggtaaac tgtgggaggc gctttacgcc ccgactggtc
     1381 aaagcaaacc gcaagtcctc ccgaccacca tcggcgcggc agcgcctgta gggacggctc
     1441 tccgagccgt cctgatttac gcccccgctt cgatccgcaa tttagcggat catcgtcgcc
     1501 tcgacgttaa acgtcctcac gcgtttcgag cggctgcgac gcgtctcacc cggggcaatg
     1561 cagtaggaaa ccactgatga acaccgatgg acacggattg aaaactcttt ccataaggaa
     1621 aaagtcattt gaaagactca ctctttggtg aagacaaacg tgtcgcgtta gcccgcgttc
     1681 atcacacgca gatgcggggg tgcgcgcagt ctg
//
LOCUS       DASPLT010001662         3148 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_122052, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001662 DASPLT010000000
VERSION     DASPLT010001662.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 3148)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3148)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3148
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_122052"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2034)
                     /locus_tag="VEA63_16910"
     CDS             complement(<1..2034)
                     /locus_tag="VEA63_16910"
                     /inference="COORDINATES: protein motif:HMM:NF012245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HYD85752.1"
                     /translation="MKLTIGKKIGLGFASLLAILAVSGTYAVVKMKQAAVGSRHVSDE
                     YMPELQFTDALNAGVAAANLNARTFALSGDEQYLELARKAFTEVEAAFKQGDALAANS
                     TQLVKLKAGIERGQAIFAGYVKLVEETAATEDEIERVRIESDKAATAAVEGHGALLAG
                     QFAALDSEIVSGAPAAALNERRAKILSLEKIGTLFTQTRLANLRSQAVRDPAILKAAL
                     TNYSRIAEEIAALMPSMRQPADVAELNGVKQQAAVYQQALTTQLATYERMSEIAVRRA
                     RSLADMDAFSTELTHAALEAGTAVSRENTSALNASANLMVACVIIALVVGIAVAATIT
                     LIITRPIARATAAVQKIATGDLTETIAVTSQDEVGQICTALNRMLESLRSVVGEVSTA
                     ANNVASGSEEMSATAQQLSQGASEQAASAEETTSSMEEMTSSIQQNADNARQTDKLAT
                     QAAADAGTGGEAVAKTVSAMKEIAAKISIIEEIARKTDLLALNAAVEAARAGEHGKGF
                     AVVASEVRKLAERSQTAAAEISKLTASGVTVAEGAGELLVRLVPDIRKTAGLVQEIAA
                     ASAEQNTGAAQVNKAIQQLDQVIQQNSSASEEMASTAEELSSQAEQLQASIGFFRLGA
                     SVQPVASLRPPTRQAKEPAVAKAAKPSKNGTHPVARNGSTGHVISLADKPRSNG"
     gene            complement(2074..2586)
                     /locus_tag="VEA63_16915"
     CDS             complement(2074..2586)
                     /locus_tag="VEA63_16915"
                     /inference="COORDINATES: protein motif:HMM:NF013731.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HYD85753.1"
                     /translation="MSNTTAIASAQYITFKLGDELFAIDVAQVREVLELPLITRVPTA
                     PAYMRGVVNVRGKAVPIVDLRAKFGLPSTADTLNSRIVVMELMLDGESTVVGGVADSV
                     HEVIELEGSQIEPPPRIAMRWRTELIRGMGRRGDEFIIILDIEAVFSLAELSAVVGGA
                     DAANSAGPAS"
     gene            complement(2610..>3148)
                     /locus_tag="VEA63_16920"
     CDS             complement(2610..>3148)
                     /locus_tag="VEA63_16920"
                     /inference="COORDINATES: protein motif:HMM:NF013731.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HYD85754.1"
                     /translation="VKREIEALGGSLHIASERGKGTTISATLPLTLAIIDGLLVAIGS
                     DQFIIPMSAVQENVELARGDRARNNGRNVVPVRGELVPYVWLRGTFASTGEEPELEKI
                     VIVRHGEDRVGLVVDRVLGSHQTVIQSLGRFYRGIEVLSGATIMGDGRVALILDVPGL
                     VRFVEKQNAAERVGADAR"
BASE COUNT          530 a         1046 c          957 g          615 t
ORIGIN      
        1 gccgttgctc cgcggcttgt ccgccaggct gatgacgtgc ccggtggagc cgttgcgcgc
       61 aaccgggtgc gtgccgtttt tggacggctt cgcagccttc gcgacagcgg gctccttcgc
      121 ctgacgtgtc ggaggacgaa gcgaagccac cggctgcacg ctcgcgccga gccggaagaa
      181 cccgatcgaa gcttgcaact gctccgcctg gctcgacagc tcttcagccg tcgaagccat
      241 ctcctcggac gccgaggagt tttgctgaat cacctgatca agttgctgga tcgctttgtt
      301 cacctgcgct gcgcccgtgt tttgctcggc gctcgccgcg gcgatttcct ggacgagacc
      361 cgcggtcttg cgaatgtccg gcacgagccg caccaacaac tcgcccgcac cttcggcgac
      421 ggtcacgccg ctcgcagtga gcttgctgat ctcagccgcc gccgtctggc tgcgttccgc
      481 gagcttgcgc acttcggacg cgaccacggc aaaacccttc ccgtgctcgc ccgcgcgcgc
      541 cgcttccaca gcggcattga gcgcaagcag gtcggtcttc cgcgcgatct cttcaatgat
      601 gctgatcttc gccgcgattt ctttcatcgc gctcaccgtc ttcgcgacgg cttcgccgcc
      661 ggtgccggca tcggccgcgg cttgcgtcgc gagcttgtcg gtctgacgtg cgttgtcggc
      721 gttttgttga atgctcgagg tcatctcttc catcgacgac gtagtttcct ccgccgacgc
      781 cgcttgttcg ctcgcgcctt gcgacaactg ctgcgcagtc gcactcattt cctcggagcc
      841 cgaggcgacg ttgttcgccg cagtcgagac ctcgccgacc accgatcgga ggctctccaa
      901 catgcggttc aatgcggtgc agatctgacc gacctcgtcc tgcgacgtga cagcgattgt
      961 ttcagtgaga tcgccagtgg cgattttctg caccgctgcc gtcgcacgcg cgatcggtcg
     1021 cgtgatgatc aacgtgatcg tagccgcgac cgcgatcccg accaccaacg cgatgatgac
     1081 gcatgccacc atgagattgg cggacgcgtt cagcgccgac gtgttttcac gcgacactgc
     1141 ggtcccggcc tccaacgccg cgtgggtcag ttcggtggag aacgcatcca tgtccgcaag
     1201 cgagcgcgca cggcgcaccg cgatttcgct catgcgttca tatgtcgcca actgcgtggt
     1261 caatgcctgc tgataaacgg ccgcctgctg cttcacgcca ttaagctcgg caacgtcagc
     1321 cggttgccgc atcgacggca taagtgcggc gatctcttcg gcgatgcggc tgtagttcgt
     1381 cagcgcagcc ttgagaattg ccggatcgcg cacggcttgc gaacgaaggt tcgcgagacg
     1441 cgtctgggtg aagagggtgc caatcttctc gagcgaaaga atctttgccc gacgctcgtt
     1501 gagggcggcg gccggcgcgc cggagacgat ctcggaatcc agcgccgcaa attggccggc
     1561 gagcaacgca ccgtgcccct ccacggcggc ggtcgccgct ttgtccgact caatccgaac
     1621 gcgctcgatc tcgtcctcgg tcgcagccgt ctcttcaacc aatttgacat agccggcgaa
     1681 gatcgcctgc ccacgttcaa tcccggcttt cagcttcacc aattgcgtgc tgttggcggc
     1741 aagggcgtcg ccctgtttga acgctgcttc gacttcagtg aacgctttgc gcgcaagttc
     1801 gagatactgc tcgtcgccgc tcagcgcaaa ggtgcgtgcg ttgagattcg ccgcggcaac
     1861 gccggcattg agcgcgtcgg tgaactgcag ctccggcatg tattcgtcgg atacatggcg
     1921 cgaaccgacg gcggcctgct tcatcttgac gaccgcatac gtgccgctga cggcgagaat
     1981 cgcgaggagc gaggcaaagc ccaacccgat cttctttcca atagtgagtt tcataatcga
     2041 acgaactgcg tgatttaaaa tgtccgtgtg cggtcagctt gccggccctg cgctgttggc
     2101 tgcgtcggcg ccgccgacca ccgcgctcaa ctccgccaag gagaacaccg cctcgatatc
     2161 gaggatgatg atgaactcgt cgccgcggcg gcccatgccg cggatcaact cggtgcgcca
     2221 gcgcatcgcg atgcgcggcg ggggttcgat ttgcgagcct tcgagctcga tcacctcgtg
     2281 cacgctgtca gcgacgccgc cgaccaccgt cgactcgccg tcaagcatca gctccatcac
     2341 gacgatgcgc gagttgagcg tgtccgccgt cgagggcaga ccgaatttcg cgcgcaaatc
     2401 gacaatcggc acggctttgc cgcgcacgtt gacgacgccg cgcatgtaag cgggggcggt
     2461 cggcacgcgt gtgatcagcg gcagctcgag aacctcgcgc acctgcgcga cgtcgatggc
     2521 gaagagttcg tcgcccagtt tgaacgtgat gtattgggcg gaagcgatgg cggtggtgtt
     2581 gctcatgttt aaaatgaaaa aagttcggtt cagcgggcgt cggcgccgac gcgctccgcg
     2641 gcgttttgtt tttcgacgaa ccgcacgagg ccgggcacgt ccagaatcaa cgccacgcgg
     2701 ccgtcaccca tgatcgtcgc acccgaaagc acctcgatgc ctcgataaaa gcggccgagc
     2761 gattggatca ccgtttgatg gctgccgagc acgcgatcga cgacgagccc gacgcggtct
     2821 tcgccatggc gcacgatcac aatcttctcg agctccggct cttcacccgt gctcgcgaac
     2881 gtcccgcgca accagacata cggcacgagt tcgccgcgca ccggcacgac gttgcggccg
     2941 ttgttccgcg cgcggtcacc gcgcgcgagc tccacgttct cctgcacggc cgacatcgga
     3001 atgatgaact ggtcggaacc gatcgcgacg agcagcccat cgatgatcgc gagcgtcagc
     3061 ggcaacgtcg ccgagatcgt cgtgcctttg ccgcgctcgc tcgcgatgtg aagcgagccg
     3121 ccgagcgcct cgatctcccg cttcacga
//
LOCUS       DASPLT010001663         1409 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_59965, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001663 DASPLT010000000
VERSION     DASPLT010001663.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1409)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1409)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1409
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_59965"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            162..440
                     /locus_tag="VEA63_16925"
     CDS             162..440
                     /locus_tag="VEA63_16925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD85755.1"
                     /translation="MEPASSAENVSVLPTLKVTAAQERALKQEITERVNQSAGGAPAI
                     APPLAELTIFTPDFRDDSKPAAPSCGSAFSPAAKRIGVAQVESDIPSG"
     gene            complement(464..1036)
                     /locus_tag="VEA63_16930"
     CDS             complement(464..1036)
                     /locus_tag="VEA63_16930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4256 domain-containing protein"
                     /protein_id="HYD85756.1"
                     /translation="MKGAKTGETLSPKQREELLETLRARFEKNMNRHKGVAWADVKAR
                     LEANPGKLWSLSEMERTGGEPDVVGLDKKTGECVFFDCSPQSPAGRYSLCYDREALEA
                     RKKFKPKDSAMDLAAAIGIELLTEEQYAELQKLGEFDTKTSSWLKTPPEIRKLGGAIF
                     GDRRFGRVFTYHNGADSYYSGRGFRGSLRV"
     gene            1035..1181
                     /locus_tag="VEA63_16935"
     CDS             1035..1181
                     /locus_tag="VEA63_16935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD85757.1"
                     /translation="MNFLLLPVDPIASLKRATAAMALRYPKFVNEDNKGGDGPNDEAF
                     ARGW"
BASE COUNT          271 a          457 c          386 g          295 t
ORIGIN      
        1 gtggaatcgt cgccaacacc tcccgcctct cttattccgc acccgagaaa aaaggacggc
       61 gaccccggcg gtcgcgtaac catcgaccaa cgggtcgaat tgcgatgctc agccgttcgt
      121 tgcgtttagt cgcagctttt attccgtgcg gcgaggtcgc cgtggaaccg gccagctcgg
      181 cggaaaacgt cagcgtgctc cccaccctca aggtgaccgc cgcacaagaa cgcgcgctga
      241 aacaggagat cacggagcgc gtgaaccagt cggccggcgg tgcaccagcg atcgccccgc
      301 cgctcgctga gttaacgatt tttaccccgg atttccgaga cgattccaag ccggctgcgc
      361 catcgtgtgg tagcgcgttt tcgcccgcag cgaaaagaat tggagtcgcg caggtggaat
      421 cggacatccc gtcgggttga ttccaacgcc agccgctcgg tcgttaaacc ctcagcgaac
      481 cacgaaatcc tcgaccgctg taatacgagt ccgcgccgtt atgatacgtg aaaacgcgac
      541 cgaagcgtcg gtcgccgaaa atcgcgccgc caagcttgcg aatctccggc ggcgttttca
      601 gccagctcga cgtcttcgtg tcgaactcgc cgagcttctg cagctcagcg tattgttctt
      661 cggtcagcag ctcgatgccg atggccgcgg ccaaatccat cgcgctgtcc ttcggtttga
      721 atttcttccg ggcctccagg gcttcgcggt cgtagcagag gctgtagcgg cccgccgggc
      781 tttgcggcga gcaatcgaag aacacgcatt cgcccgtttt cttgtcgagg ccgacgacat
      841 ccggctcccc acccgtccgc tccatctcgc tgagcgacca aagttttccc gggttcgctt
      901 ccaaacgcgc tttcacgtcg gcccatgcga cgcctttgtg gcggttcatg tttttctcga
      961 aacgggcccg caaggtttcg agcagttctt cgcgttgttt gggagacaac gtctctccgg
     1021 ttttagcgcc tttcatgaat ttccttttgt tgccggtcga tccgatcgct tctttgaaac
     1081 gagccaccgc agcgatggcc cttcgctatc caaagttcgt caatgaagat aacaagggcg
     1141 gcgacggacc aaacgacgag gcttttgcgc gcggctggta aaagcgatcg tttcctccga
     1201 aaccccacca ttcgtttttt cgggccatgc cccacagcac cggacggcgc acaggagtaa
     1261 attcggccat gaaacgctcc attccaacca ccatgaaggc cgcggccatc gaccacttcg
     1321 gtggtcccga tgtcctgcgg ctgcaatctc tcgacgtgcc gacaccgaag cccaacgagg
     1381 tgctcattca actggatacc gccggcatc
//
LOCUS       DASPLT010001664         1530 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_60023, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001664 DASPLT010000000
VERSION     DASPLT010001664.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1530)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1530)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1530
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_60023"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            273..794
                     /gene="lptE"
                     /locus_tag="VEA63_16940"
     CDS             273..794
                     /gene="lptE"
                     /locus_tag="VEA63_16940"
                     /inference="COORDINATES: protein motif:HMM:NF016290.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HYD85758.1"
                     /translation="MTRCLPSPGLLLLLVALFSLTGCANYQLGTEGRLTFTTLYIEPV
                     ENQTLLPQARTIVSTHIREAFEQDGRVSLASSPETADATLHVVLRDYHRDVTSVRESD
                     TALARKLTLTLAADCTLHDRRTGQTLFANRTLTSQRDAFTDGGQLQSEYQTLPLLAQS
                     LAAKVTHAVLDVW"
     gene            808..1464
                     /gene="rpe"
                     /locus_tag="VEA63_16945"
     CDS             808..1464
                     /gene="rpe"
                     /locus_tag="VEA63_16945"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HYD85759.1"
                     /translation="MHPPLLAPSLLAGDHAHLASSARLVEELELAWLHVDIMDGHFVP
                     NLTFGPETVAALRRHGLKLFFDTHLMLDEPHRYVEAFAKAGANLISIHIEPRYDHVAT
                     LARVRDLGCQAGIVLNPDTPAAAVEPFLAHVDLVLVMTVQPGFGGQPFRDDMLPKLRQ
                     IDTWRRERNLPFRLEVDGGIDLKTAAACRAAGADTFVAGTSFFKADDKRAYAATMAAL
                     "
BASE COUNT          265 a          597 c          378 g          290 t
ORIGIN      
        1 aggcgatcac cgcgtatcca gattccgagg tcgctgcggc cgctcgcgag aagctcgcgc
       61 aagtcgaagc cagcgccagc ggcgcgccgc tgccgaagaa caccgacaag aaacggaaac
      121 gcttcttctt tttctgagcc tacgggcatc gcctttctcc cagccgtggc actgcgccac
      181 ggctttttta ttgtcgttgc ggaaccaacc cgccgccctc ggcggaactc tgacttgccc
      241 cgcttcccct cctgcccttc ccttcgcgcg gcatgacacg ctgccttcct tctcccgggc
      301 tcctccttct gctcgttgcg ctgttcagcc tcaccggctg cgccaactac cagctcggca
      361 ccgaaggccg gctcacgttc accacgctct acatcgagcc cgtggagaat cagacactgc
      421 tcccgcaagc ccgcacaatc gtgtccacac acattcgtga agcattcgag caggatggcc
      481 gcgtctctct cgcgtcatct ccggaaacgg ccgatgccac gcttcacgtc gtcttgcgcg
      541 actaccatcg cgacgtcacc tcagtgcgcg aatctgacac cgccctcgcc cgcaagctca
      601 ccctcaccct cgccgccgat tgcacgcttc acgaccgccg caccggccaa actcttttcg
      661 ccaaccgcac gctcacctcg caacgtgacg cgttcaccga cggcggacag cttcaatccg
      721 aataccagac gctcccgttg ctcgcgcagt ccctcgcggc caaagtcact cacgccgtgc
      781 tggacgtctg gtagtctctc ttccgcgatg cacccgccgc tcctggctcc ttccctcctc
      841 gctggcgacc acgcgcacct cgcctcaagc gctcgcctcg tcgaggaact cgagctcgcc
      901 tggttgcacg tcgacatcat ggacggccac ttcgtcccga atctcacctt cggaccagaa
      961 acagttgccg cgctgcgtcg ccacggcctc aagcttttct tcgacacgca cctgatgctc
     1021 gacgaaccgc accgctacgt cgaagccttc gccaaggccg gcgcgaatct catcagcatc
     1081 catatcgagc cgcgttacga ccacgtggcg acgctcgcac gagttcgcga tctcggctgc
     1141 caagccggta ttgtgctcaa ccccgacacg cccgccgcag cggtcgagcc gtttcttgct
     1201 cacgtggatc tcgtgctcgt catgaccgtg caacctggct ttggcggaca acccttccgc
     1261 gacgacatgc tgccaaagct tcgccagatc gacacctggc gccgggaacg aaacctcccg
     1321 ttccggctcg aagtcgacgg cggcatcgac ctcaagaccg ccgccgcttg ccgtgctgcc
     1381 ggcgctgata ctttcgtcgc tggcacttcg tttttcaaag ccgacgacaa acgcgcctac
     1441 gccgccacca tggcggcact ttaacaccca ctgtctgcct tcgcccacgc atgccgcacg
     1501 agcttcgcca aggcgactac ctcatcagcg
//
LOCUS       DASPLT010001665         1282 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_244734, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001665 DASPLT010000000
VERSION     DASPLT010001665.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1282)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1282)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1282
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_244734"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1268
                     /locus_tag="VEA63_16950"
     CDS             <1..1268
                     /locus_tag="VEA63_16950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD85760.1"
                     /translation="RMAIPSNESLKHAHALLRLRHPNAERRQEAKAELEEIAARNPAV
                     ALQIHREFAADALQRNDYDELQRRQALILADPRANFTDQLQKANVDMLVNHQPFEALF
                     PQLALRATGNEGDAVQFVQWLLVQNRAGEAAGWLEQLPVDVRSHPTVKSVEADAIAQL
                     GDWDRLARLLETGAWGPIMPATLRLALAANTIDSPTRPSLRREAWDMTVESAGGNLGS
                     LRVLQRLASLWKWTDEYERTLWIIARGFPDQTWAHQSLFNLYKEKKDARSMREVMGLL
                     RSADAAVPRYQHDWALLSMLIEPNSNWNAAKDTATRLHELYPDNATYATAYAFALAQS
                     GKASEALAVVEKLSDSDRGYLPRQPYLAYIYGVTKKRAEMEKAIALAEGVPYLAEESY
                     LFVRAREELERKPSKPITPDKASGGSSKA"
BASE COUNT          272 a          441 c          348 g          221 t
ORIGIN      
        1 tgcgcatggc gattccaagc aacgagtcct tgaagcatgc ccacgcgctc ctccgtttgc
       61 gccatcccaa cgcggaacgg cggcaggaag caaaagccga attggaggaa atcgccgcgc
      121 gcaatcccgc cgttgccctc cagatccatc gcgagttcgc cgccgacgca ctccaacgaa
      181 atgactacga cgaactccaa cgtcggcagg cgctcatcct tgctgatcct cgcgcaaatt
      241 tcaccgacca gctccaaaag gcgaacgtcg acatgctcgt caaccaccag cctttcgaag
      301 ccctgtttcc gcaactcgca ctgcgggcca ccggaaacga gggcgatgcc gttcagtttg
      361 tccagtggct gctcgtgcag aaccgggctg gtgaagccgc cggctggctt gaacaactgc
      421 ccgtcgacgt tcgctcgcac cccacggtga aatccgtcga agccgacgcg atcgctcaac
      481 tcggggactg ggaccgcctg gcccgattac tcgaaaccgg tgcctggggc ccgatcatgc
      541 cggcgaccct ccgcctcgcg ctcgccgcga acaccatcga tagtccgacc cggccctctc
      601 tgcgacgcga agcttgggac atgaccgtcg agtccgccgg cggaaatctc ggatcgctcc
      661 gcgttctcca gcgactcgcg agcctctgga aatggaccga cgaatacgaa cgcacgctct
      721 ggatcatcgc ccgcggtttc cccgatcaga catgggcgca ccagtcgctc ttcaatcttt
      781 ataaggaaaa aaaggacgcg aggagcatgc gtgaagtgat ggggctgctg cgctccgcgg
      841 acgccgcggt gcctcgctat cagcacgatt gggcgctgct cagcatgctc atcgaaccga
      901 actccaattg gaacgctgcc aaggacaccg cgactcgtct ccacgagctc tatcctgaca
      961 atgccactta cgcgaccgcc tacgcgttcg ccctcgccca aagtggcaaa gcctctgaag
     1021 cgctcgccgt tgtcgagaaa ctatccgatt ccgaccgcgg atacctgcct cgccagccct
     1081 acctcgccta catttatggc gtaaccaaaa aacgcgcgga aatggaaaaa gccatcgctc
     1141 tagcggaagg cgtgccctat ctggccgaag aatcctatct tttcgtccgg gctcgtgagg
     1201 agctggagcg caaaccgtcc aagccgatca cgccggacaa agcatctggc ggctcgtcca
     1261 aagcgtgaag cgcttcctcc gg
//
LOCUS       DASPLT010001666         2269 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_122113, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001666 DASPLT010000000
VERSION     DASPLT010001666.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 2269)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2269)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2269
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_122113"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            138..938
                     /locus_tag="VEA63_16955"
     CDS             138..938
                     /locus_tag="VEA63_16955"
                     /inference="COORDINATES: protein motif:HMM:NF024758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HYD85761.1"
                     /translation="MKRFIERFLFVAALAGASVVGTTHLSAAPADDAAVGRKLVERYA
                     DAVVGVELVVTIKVKMGEREMPPREQKVEINGTMIAANGLTVTSLAEVDPQMAFEAMS
                     ANQRGARPELLGADFKEVKLRLADGREVPARFVLKDADLDLAFMAPEADAETARAFTH
                     VDLANAAEGALLEDYFIVSRAPKVLQRVPMARMTEIMGIIERPRRLYLTTEQALGTPM
                     FDAEGRVLGITFQHFANSRRTGFVVLPAEDIAEIAKQAEAAADAPASP"
     gene            complement(1214..>2269)
                     /gene="rimO"
                     /locus_tag="VEA63_16960"
     CDS             complement(1214..>2269)
                     /gene="rimO"
                     /locus_tag="VEA63_16960"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HYD85762.1"
                     /translation="AKERGTKKEAPVSFIEGKSTYIPDYDTPRFRLTPKHFAYVKIAE
                     GCNHPCTFCIIPQIRGRHRSRTVDSVVAEARQLVKEGVKELNLISQDTTFFGMDTWEQ
                     RPNPRTPVDSSRGTALTTLLRQLNAIEGDFWIRLLYTHPAHWSDELIRTIAECPKVAR
                     YIDIPLQHISDPMLERMQRETSGDYIRDLIKRIRAGIPGIAVRTTFIVGFPGETDADV
                     DELCDFIRETKFERLGVFRYSQEEGTRGAKMPDQIAPKVKEARWHRLMALQKTIAADV
                     SKTYVGRTLKVLVEEPGVARGEADAPDIDGRVYVPRELPAGEFVDVEITGYQDYDLLA
                     LPKGQRPVEFKVARQAQ"
BASE COUNT          400 a          568 c          858 g          443 t
ORIGIN      
        1 aagccggtgg agacgctggc ggagttgaaa gcggcgcggg aagcggcgac gcgcagcggg
       61 caggaatggt gggtgctgtt ggtgaagcgg cgcggtcaga cactctttgt ggagatcaac
      121 ctcaagcccg tgaaatcatg aaacgtttta tcgaaagatt tttgttcgtc gcagcgttgg
      181 cgggagcgag cgtggtgggc acgacgcacc tgagtgcggc gccggcggac gacgcggcgg
      241 tggggcgaaa gctcgtcgaa cgttatgccg atgcggtggt gggcgtggaa ctcgtcgtca
      301 cgatcaaagt gaagatgggc gagcgtgaga tgccgccgcg ggagcagaaa gttgagatca
      361 atggcacgat gatcgcggcg aacggactga cggtgacctc gctggcggag gtcgatccgc
      421 agatggcgtt cgaggcgatg agcgcgaatc agcgcggcgc gcggccggag ttgctcggcg
      481 cggatttcaa agaggtgaag ctgcggctcg cggatggcag ggaagtgccg gcgcgcttcg
      541 tattgaagga tgcggatctc gacttggcgt tcatggcgcc ggaagcggac gcggagacag
      601 cgcgggcgtt cacgcatgtg gatctcgcga atgcggcgga gggcgcgttg ctcgaggact
      661 atttcatcgt gagtcgcgcg ccgaaggtgt tgcagcgcgt gccgatggcg cggatgacgg
      721 agatcatggg catcatcgaa aggccccggc ggttgtatct gacgacggag caggcgctgg
      781 gcacgccgat gttcgatgcg gagggacgcg tgttgggcat cacgtttcaa catttcgcga
      841 acagccggag aacgggtttt gtggtgttgc cggcggagga catcgcggag atcgcgaagc
      901 aggcggaagc ggcggcggat gcgccggcga gcccgtagca cggggctctt ggagagcatg
      961 cggtaatttt tctgacccta tttttctgcc ggatagatcg tcactgcagg ccgctcgcct
     1021 gactccgatc agttggcgag aacaccaatt tggcagaaaa atgggggcag aaaaattgtg
     1081 cggatgtcag gtgtgtggct tcgagtggag cccttgaggg ctcgtgggct gtgcgtgacc
     1141 tgatgactgc cggcgggggc ggcttgcgtg tgtcaaaaag ggacgcggtc agccggcgaa
     1201 agcgcagctt cgattattgc gcctggcggg cgactttgaa ttcgacgggg cgctggcctt
     1261 tcggcagcgc gagcaagtcg tagtcttgat aaccggtgat ctcgacgtcg acgaattcgc
     1321 cggcggggag ctcgcgcgga acgtaaacgc gaccgtcgat gtcgggggcg tcggcctcgc
     1381 cgcgggcgac gccgggttct tcgacgagca ctttcagcgt gcggccgacg taggttttgc
     1441 tgacgtccgc ggcgatggtt ttctggagcg ccatgaggcg atgccagcgg gcttccttca
     1501 ccttcggcgc gatctggtcg ggcatcttcg cgccgcgcgt gccctcttcc tgagaataac
     1561 ggaaaacacc gaggcgctcg aatttcgttt cgcggatgaa gtcgcaaagc tcgtcgacgt
     1621 cggcgtcggt ttccccgggg aatccgacga tgaaggtggt gcggacggcg atgccgggga
     1681 tgccggcgcg gatgcgttta atgaggtcgc gaatgtaatc gccgctggtt tcgcgctgca
     1741 tgcgctcgag catcggatcg ctgatgtgct ggagcgggat gtcgatgtag cgggcgacct
     1801 tcgggcactc ggcgatcgtg cggatgagtt cgtcgctcca atgcgcggga tgcgtgtaga
     1861 ggaggcgtat ccaaaaatcc ccttcgatcg cgttgagctg gcgaagcagc gtggtgagcg
     1921 cggtgccgcg ggaggagtcg acgggcgtgc gcgggttggg acgctgttcc caggtgtcca
     1981 tgccgaagaa cgtcgtgtcc tgcgagataa ggttgagctc tttgacgccc tccttgacga
     2041 gctggcgggc ctcggcgacg acgctgtcga cggtgcggct gcggtggcgg ccgcgaattt
     2101 ggggaatgat gcagaaggtg cacggatgat tgcagccctc ggcgattttg acgtaggcga
     2161 aatgtttcgg cgtgagacgg aagcgaggcg tatcgtagtc gggaatgtaa gtcgacttgc
     2221 cctcgatgaa ggagaccggc gcttcttttt tcgtgccgcg ttccttcgc
//
LOCUS       DASPLT010001667         1047 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_122117, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001667 DASPLT010000000
VERSION     DASPLT010001667.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1047)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1047)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1047
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_122117"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..630
                     /locus_tag="VEA63_16965"
     CDS             <1..630
                     /locus_tag="VEA63_16965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite reductase"
                     /protein_id="HYD85763.1"
                     /translation="EVAQKFPHVEFRLSANQNVIIANVPESDRDGITAVLASHGVKVE
                     NQASVLHAASMACPALPTCGLALAESERMLPGLIDRIEKLGGELGLGGEEIIIRSTGC
                     PNGCARPYMAEIAFVGKAPGRYQIWLGGNEAGTRLNRVYKDTVKDPDIENELRPILTR
                     WKAERVANERFGDFAARVLWPEIETAAAVAAEAAAKTPVPATTAPGPTL"
     gene            696..>1047
                     /locus_tag="VEA63_16970"
     CDS             696..>1047
                     /locus_tag="VEA63_16970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase family
                     protein"
                     /protein_id="HYD85764.1"
                     /translation="MSPDQIAAANADLRSKSPLEIVRWAIAQAPGRAIVSTNFRPYEA
                     VVLHLATQVQPDIPVLWVDHGYNRPATYKHAEQLRAQLKLNLKPFLPKMTPAHRDAIF
                     GEIPTTEDEAGLKKF"
BASE COUNT          209 a          409 c          262 g          167 t
ORIGIN      
        1 gaagtcgcgc agaaattccc gcacgtggaa ttccgcctct cggcgaacca gaacgtcatc
       61 atcgccaacg tccccgagtc cgaccgcgac ggcatcaccg ccgtcctcgc gtcgcatggt
      121 gtgaaggtcg agaatcaggc gtccgtcctc cacgccgctt ccatggcctg ccctgcgctt
      181 ccgacttgcg gcctcgcgct cgccgagtcc gagcgcatgc tccccggcct catcgaccgc
      241 atcgaaaaac tcggcggcga actcggcctt ggcggcgaag agatcatcat tcgttccacc
      301 ggctgcccca acggctgcgc gcgtccttac atggccgaaa tcgctttcgt cggcaaagct
      361 cccggccgtt accaaatctg gctcggcggc aacgaagctg gcacccgcct caaccgcgtt
      421 tacaaggaca ccgtcaagga tcccgacatc gaaaacgaac tccgcccgat cctcacgcgt
      481 tggaaagccg agcgcgtcgc caacgagcgc ttcggcgatt tcgccgcccg cgtcctctgg
      541 ccggagatcg aaaccgccgc agccgtcgcc gccgaagccg ccgcgaaaac cccggtcccc
      601 gccacgaccg ctcccggacc cacgctctag aggtagggac gtctctccga gacgtccgct
      661 ccgatctccc aactccgatc tccgactccg aaaaaatgtc ccccgaccaa atcgccgccg
      721 ccaacgccga cctccgctcg aaatcccctc tcgaaattgt tcgctgggcc atcgcccagg
      781 cgccgggccg cgcgattgtt tcgacgaact tccgtcctta cgaggccgtc gtccttcacc
      841 tcgccacgca agttcagccc gacatccccg ttctctgggt cgaccacggt tacaaccgtc
      901 ccgcgaccta caagcacgcc gaacaactcc gcgcacaact caagctgaac ctgaaaccgt
      961 ttctcccgaa aatgacgccc gctcaccgcg acgccatctt cggcgaaatc ccgacgaccg
     1021 aggacgaagc cggcctgaaa aaattca
//
LOCUS       DASPLT010001668         1860 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_81198, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001668 DASPLT010000000
VERSION     DASPLT010001668.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1860)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1860)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1860
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_81198"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(115..>1860)
                     /locus_tag="VEA63_16975"
     CDS             complement(115..>1860)
                     /locus_tag="VEA63_16975"
                     /inference="COORDINATES: protein motif:HMM:NF027452.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4838 domain-containing protein"
                     /protein_id="HYD85765.1"
                     /translation="IDTVGNESNHPGKRFIYVGENTVSTAAGISTAALDNEFFVIRTV
                     GGDLHIVGKDAGNNDWTQLSNCQPGTMFGVYHVLSEVLGVRWLWPGDDGITYTAKANV
                     DLPSLNITTGPNMVQRRYRNPRVGMYNNGITSYGFGIPVLPASSTVREALADEEILWQ
                     RRQMMGTRKNPSFGHSELHWWDTYGATHPEYFAVRLNNAPQPAPSPDRVKLHVSGVDT
                     INARVAEWVAAGAPNNFNVCPNDSRAFCVCASCLAWDYPSQPATTVYNNSDALLGDRY
                     ARWYSEIATRVAAINPNATVYGYAYDTYRNAPTVATVPSNVAIAYIPGPPSDVRPALI
                     AETEANVLGWIAAGATKMYLRPNWMLSAHAGPYVPVHRVGEHFKTLLSGGYLLGMDSD
                     SSNSTYAGFGLYFYLIAQQMAHPERSVDTIIDEYCAGFGSASSRIRDYFQYWENFIYH
                     ESDNGNTDILGWTAGMAAYGPTYTNAAFDGAEQILDDAAAQLGSGEIEAAGRIEFLRT
                     AVTHGRLTAEAIRLVEPGTPVRYNPSADAAMRALLAFRDSEADQFTIWREWTIDRESY
                     VPGMQSYWEYILANP"
BASE COUNT          318 a          521 c          655 g          366 t
ORIGIN      
        1 tgaatggccg cactttgatg ccacctctcc ggacctcgag gcgttggcgg ctggcgttaa
       61 acgaaaaagg ggcgccttgc ggcgcccctc ggggattcga tggctcgatc gcgtttacgg
      121 atttgcgagg atgtattccc agtaggattg catgccggga acgtaggatt cgcgatcgat
      181 cgtccactcg cgccaaatcg tgaactggtc ggcttcgctg tcgcggaagg cgagcagcgc
      241 gcgcatcgcg gcgtccgcgg atggattgta acggacgggt gtgccgggct cgacgaggcg
      301 gatcgcctcg gcggtcaggc ggccgtgagt gaccgctgtc ctcaaaaact cgatacggcc
      361 ggcggcctcg atttcgccgg agccgagctg ggcggcggcg tcgtcgagga tttgttcggc
      421 gccgtcaaat gcggcgttcg tgtaggttgg accgtaggcg gccataccgg cggtccaacc
      481 gaggatgtcg gtgtttccgt tgtccgactc gtggtagata aagttctccc agtattggaa
      541 gtagtcgcgg atccgcgacg aggcgctgcc gaagcccgcg cagtattcgt cgatgatcgt
      601 gtcgaccgag cgttccggat gcgccatctg ctgggcgatg aggtagaagt agaggccgaa
      661 accggcgtag gtggaattcg agctgtccga atccatgccc aggaggtaac cgccgctgag
      721 cagagtcttg aaatgttcgc cgacgcgatg gacgggcacg taaggaccgg cgtgcgcgga
      781 gagcatccag tttggacgga gatacatctt cgtcgcgccg gcggcgatcc agccaagcac
      841 gttcgcctcg gtctccgcga tcagggcagg tcgcacatcg ctcggaggtc cggggatgta
      901 ggcgatcgct acgttcgacg gcacggtggc gaccgtcggc gcgttgcgat acgtgtcgta
      961 ggcgtagccg tagacggtcg cattcggatt gatcgcggcg acgcgcgtgg cgatttccga
     1021 ataccagcgc gcgtagcggt cgccgagcaa ggcgtcgcta ttgttgtaaa cggtcgtggc
     1081 gggctgggag gggtaatccc acgcgaggca gctcgcgcag acgcagaatg cgcggctgtc
     1141 gttcgggcag acgttgaagt tgttcggagc gccggcggcc acccattcgg cgacgcgcgc
     1201 attgatcgta tcgacgccgc tgacgtgcaa cttcacgcga tccggcgagg gtgcgggttg
     1261 gggcgcgttg ttgaggcgca ccgcgaaata ctccggatga gtggcgccgt aagtgtccca
     1321 ccagtgcaac tccgagtggc cgaacgaagg gttcttgcgc gtgcccatca tttgccggcg
     1381 ctgccagagg atttcctcgt ccgcgagcgc ttcgcggacc gtgctgctcg ccggcaatac
     1441 cggaatgccg aagccatagc tcgtgatgcc gttattatac atgccgacgc gcggattgcg
     1501 gtaacggcgc tgcaccatgt tcgggccggt cgtgatgttg agtgacggca ggtcgacatt
     1561 ggccttggcg gtgtaagtga tgccgtcgtc accgggccag agccagcgga cgccgagcac
     1621 ttcgctgagc acgtgataaa cgccaaacat cgttccgggt tggcaattcg agagctgggt
     1681 ccagtcgttg ttacccgcgt ccttgccgac gatgtgaaga tcgccgccga cggtgcggat
     1741 gacgaagaac tcgttatcga gcgcggcggt gctgatgccg gccgccgtgc tgacggtgtt
     1801 ctcaccgacg tagatgaagc gcttgccggg gtggttgctt tcgtttccga ccgtgtcgat
//
LOCUS       DASPLT010001669         1549 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_101603, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001669 DASPLT010000000
VERSION     DASPLT010001669.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1549)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1549)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1549
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_101603"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1425
                     /locus_tag="VEA63_16980"
     CDS             <1..1425
                     /locus_tag="VEA63_16980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 20"
                     /protein_id="HYD85766.1"
                     /translation="KVHVGLDESFHLGKHPLSRREIAKIGLAAHFARYVKRLHALTQS
                     LGLEMGLWADMFYFLPDAIPLLPKGLTAYEWYYHAFNRLPRVELFNFAEVDIATPLRA
                     RGAKLYGCPMNGAFRYEPLPHFGDRMNNIVAWWRHAQRIGAEGFLVSGWEPNRLAIEL
                     TTAVDAAAASLWLEPEVTDPRGMLARGFERAFGKKKGRAAANAALACDRYPFGGYPRW
                     QINERWDAVSRREPLASYRRETRYFARLAADAERVRLAEPLRTSLGFRHYVAERDLCV
                     RQAARGDTSALVRLGRLIASGRRAAAAMWNRTRDRDSLGPNVQMVQRDAARLKAARRG
                     EPVFGGRWQLCYCVWNFAPAVQLVGVGQQQPDGSWQTLQSSHTIEFQTRSARRRADVV
                     REHAAPVTWNGDRTQPPRLRIFVRGVGEVKIDRVALNGGSAAYPARGFARWRRVGSVA
                     PTSGWPEFNWSVNREAIELSFAFR"
BASE COUNT          247 a          517 c          505 g          280 t
ORIGIN      
        1 aaagttcacg tcggcctcga cgaatcgttt catctcggca aacacccgct gagccgccgc
       61 gagatcgcca agatcggcct cgccgcgcac ttcgctcgct atgtgaaacg cctgcatgcg
      121 ctcacgcagt cgctcggcct cgagatgggg ctttgggcgg acatgtttta ctttttgcct
      181 gacgcgattc cgctgctgcc gaagggactc accgcctacg agtggtatta tcacgcgttc
      241 aaccggctgc cacgcgtcga gctgttcaac ttcgccgaag tcgacatcgc cacaccgctg
      301 cgcgctcgag gcgcgaagct ctacggctgt ccgatgaacg gcgcgtttcg ttacgaaccg
      361 ctgccgcact tcggcgatcg catgaacaac atcgtcgcct ggtggcggca cgcgcaacgg
      421 atcggcgcag aaggttttct cgtgagcgga tgggaaccga atcggctcgc gatcgagctg
      481 accaccgccg tcgacgccgc ggcggcaagc ctgtggctcg agccggaagt caccgatccg
      541 cgtgggatgc tcgcgcgagg cttcgagcga gcgtttggaa aaaagaaagg tcgcgccgcc
      601 gcgaacgcgg cgctcgcatg cgaccgatat ccgttcggcg gttatcctcg gtggcaaatc
      661 aatgagcgct gggatgccgt ttcgcgccgc gagccgctcg cgagctatcg ccgcgaaacg
      721 cgctacttcg cgcgcctcgc tgcggacgcc gagcgtgtcc gactcgcgga accgctgcgc
      781 accagcctcg gctttcgcca ttacgtggcc gagcgcgatt tatgtgtccg ccaagccgcg
      841 cgcggcgaca cttcggcgct cgttcgtctc ggacgcttga tcgcgagcgg acggcgcgcc
      901 gccgccgcga tgtggaaccg cacgcgtgat cgcgattcgc tcggccccaa cgttcagatg
      961 gtgcaacggg acgccgcgcg cttgaaagcg gcacgccgcg gtgagcccgt gtttggtggg
     1021 cgctggcagc tttgctattg cgtatggaat ttcgcgccgg ccgttcagct tgtcggcgtg
     1081 ggacaacagc aacccgacgg ctcgtggcaa acgctgcagt catcccacac gatcgagttt
     1141 caaacgcgct cagcgcgccg ccgcgccgat gttgtgcgcg agcacgccgc acccgtcaca
     1201 tggaacggcg accgcaccca gccgccgcgc ctgcggatat tcgtgcgtgg cgttggggag
     1261 gtgaagatcg accgcgtcgc gctcaacggc ggaagcgccg cgtatccagc gcgtggattc
     1321 gctcgctggc ggagagtcgg cagcgtcgct ccaacatccg gctggcccga gtttaattgg
     1381 tcggtcaacc gcgaggccat cgagctctcg ttcgccttca ggtagggcga accgtgcccg
     1441 gtgggccgtt gcaatcgcgg cccggcgacg gcgcctcgct tttcctcgtc ggcctgcgat
     1501 cggcgcgtct cagctgccgt cgtcctccac cggcaggtgt tgtgcgaga
//
LOCUS       DASPLT010001670         2040 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_244921, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001670 DASPLT010000000
VERSION     DASPLT010001670.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 2040)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2040)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2040
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_244921"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            137..1270
                     /locus_tag="VEA63_16985"
     CDS             137..1270
                     /locus_tag="VEA63_16985"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009507795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HYD85767.1"
                     /translation="MSCELALKPELRGTKCAVGGRERGIISSASYEARACGVYTPMPT
                     SRALKVCPDLIMLPHTSGLYSKVSHQMFELCETLTPIVQRNSIDEGYLDLTPCVFGSV
                     EEIERRVRELQARIWRELQITVSFGIATNKLVSQIASKLRKPRGFVVVPPGTEAAFLA
                     PLPIGKLPGVGPKTETTLVEVHGIHFVRDLLAKSEAELEAIFGAGWPEMVECARGIDR
                     RVVHAEHEDAKSYSQQETFFRDVGEFAEIERVMKRMIDELLPKIREDGKRVRTMTVKV
                     RYPDFSQESHGRSLATSTDLEAPFYPLVAPLLRTAWKKRRPLRLVSVRFSNVEDGPEQ
                     LEMFAQTDQKRRRLAEVLDRLNNRGRAAVVQHGHQLAKKPPTD"
     gene            1284..1856
                     /locus_tag="VEA63_16990"
     CDS             1284..1856
                     /locus_tag="VEA63_16990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FmdB family transcriptional regulator"
                     /protein_id="HYD85768.1"
                     /translation="MPIYEYYCPENHTIYQFYAKTLAQGKTVPKCPHNPSYRMQKLLS
                     QFAVTSGGKSGDEPPANAAPGAGGDPAEDAKMEAAMSAMEREFSSIDENDPRAMARMM
                     RRMSELTGEKIDGEMEEVVRKLEEGVDPEALEEELGGGDAEGMGDPDDPYGGGMGGEG
                     GPAPDPKEPKHRFKVRRTAPTRDPKLYDYE"
BASE COUNT          412 a          554 c          702 g          372 t
ORIGIN      
        1 atcttgcgcg atagcatact ctgagcgggc tgaagcccgc cccactaaaa tttcgctccg
       61 cttttttcag cgggtgcgcg aaggtggagg agtggtgctg ccgaacatcg ttcatctcga
      121 cgccgacgcg tttttcgtgt cgtgcgagct cgcgttgaaa ccggagctgc gtggcacaaa
      181 gtgcgcggtc ggcgggcggg agcgcggcat catctcgtcg gcgagctatg aggcgcgggc
      241 gtgcggggtt tacacgccga tgccgacgtc gcgcgcgttg aaggtatgtc cggatctgat
      301 catgctcccg cacacgtcgg gactttacag caaggtttcg catcagatgt tcgagttgtg
      361 cgaaacgctc acgccgatcg tgcagcgcaa ctccatcgac gaaggttacc tcgatctgac
      421 gccgtgcgtt tttggcagcg tcgaggagat cgaacggcgc gtgcgcgagt tgcaggcgcg
      481 aatttggcga gagctgcaga tcacggtttc gttcgggatt gcgacgaaca aactcgtttc
      541 ccagatcgca tcgaaattgc gcaagccgcg cggatttgtg gtggtgccgc cgggaacgga
      601 agcggcgttt ctcgcgcccc tgccgatcgg caagctgccg ggcgtcgggc cgaaaacgga
      661 gaccacgctc gtcgaagtgc acggcatcca cttcgtccgg gatttgctcg cgaagagcga
      721 agctgagctc gaggcgatct tcggcgccgg ctggccggag atggtggagt gcgcgcgggg
      781 aatcgaccgg cgtgtcgtgc acgccgagca tgaggatgcg aaaagttatt cgcagcagga
      841 gacgtttttc cgcgacgtcg gggagttcgc agagatcgag cgcgtgatga aacggatgat
      901 cgacgaactg ctgccgaaga ttcgtgagga cggaaaacgc gtccgcacga tgaccgtgaa
      961 agtgcgttat ccggatttct cacaagagtc gcacgggcgc agtctcgcga cgtcgaccga
     1021 tctcgaggcg ccattttatc cgctggttgc gccgttgctg cgcaccgcgt ggaaaaaacg
     1081 gcggccactg cgactggtga gcgtgcgatt ttcgaacgtg gaggatgggc ctgagcagtt
     1141 ggaaatgttt gcgcagacgg accaaaaacg gcgtcggctc gcggaggtgc tggaccggtt
     1201 gaacaaccgc ggccgggcgg cggtcgtgca gcatgggcac caacttgcca aaaagccgcc
     1261 gaccgattaa tccggttttt cgcatgccga tttacgaata ctactgtccg gagaaccaca
     1321 cgatctacca gttctacgcg aagacgctcg cgcaggggaa gacggtgccg aagtgcccgc
     1381 acaatcccag ttatcgaatg cagaagcttc tgtcgcaatt cgccgtgacg agcggcggca
     1441 agtcgggcga cgagccgccg gcgaatgcgg cgccgggagc aggcggcgat cccgccgagg
     1501 acgcgaagat ggaggcggcg atgagcgcca tggagcggga gttttcgagc atcgatgaaa
     1561 acgatccgcg cgcgatggcg cggatgatgc gacggatgtc ggaactcacc ggcgaaaaaa
     1621 tcgacggtga aatggaagaa gtcgtccgca agttggaaga aggcgtcgat cccgaggccc
     1681 tggaggagga attgggcggc ggcgatgccg agggaatggg cgatccggac gatccttacg
     1741 gtggcgggat gggcggagaa ggtgggccgg cgcccgatcc gaaggagccg aagcatcgtt
     1801 tcaaagtgcg acgcaccgcg ccaacgcgtg atccgaagct ttacgattac gagtgagatc
     1861 ggaaggcgac ggtagagcgg cggcgctttc gccggtctcc tttgattacg gtgaatcttt
     1921 agggttggtc attcgccggg gaatcgcgtc cattcgcggc gatcatgtcg cactcgtccg
     1981 aacttttgtc ccgtatcgaa gcagccaaac gcgcggtgct cgcgcagacc gaactcctcc
//
LOCUS       DASPLT010001671         5316 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_122382, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001671 DASPLT010000000
VERSION     DASPLT010001671.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 5316)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5316)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5316
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_122382"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..442
                     /locus_tag="VEA63_16995"
     CDS             <1..442
                     /locus_tag="VEA63_16995"
                     /inference="COORDINATES: protein motif:HMM:NF014841.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HYD85769.1"
                     /translation="AEAQQLNVQLVSLEEIFATADVVSCHMPLTDATQRSLGRPHFAA
                     MKPGATFINTARGLLVRENELIDVLKTRPDLHAVLDVMESEPPPPESPLFSLPNVVMT
                     PHIAGSIGTECRRMGTMILDEVQRYLAHEPLRGEVWQEQLNTLA"
     gene            650..1411
                     /locus_tag="VEA63_17000"
     CDS             650..1411
                     /locus_tag="VEA63_17000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HYD85770.1"
                     /translation="MSSFRHSVSQWCFPHIPLPDLARAARDIGIASIELLEPADWPAL
                     RPFGLICAMARGPGSIEDGFNRRENHAALVPAFLERIRECAAAGVPNVVCFSGNRRGL
                     SDEEGLDHCAAGFSQLVGAAERAGVTLCLELLNSKIDHPDYQCDHTAWGVELVKRVGS
                     ERLKLLYDIYHMQIMEGDVVRTIEQNHAYIAHYHTAGVPGRHEIDDSQELNYPRIMRA
                     IAATGFTGFVGQEFLPLRDPLTSLREAVELCTLRP"
     gene            complement(1535..2518)
                     /gene="iolG"
                     /locus_tag="VEA63_17005"
     CDS             complement(1535..2518)
                     /gene="iolG"
                     /locus_tag="VEA63_17005"
                     /EC_number="1.1.1.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR04380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050112 - inositol 2-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol 2-dehydrogenase"
                     /protein_id="HYD85771.1"
                     /translation="MLKFAQFGAGRIGAIHASNLAAAGAQLLYVVDVNELAAAKLAAT
                     HGARVADAAKVFADRNVDAVIIASSTDTHADLAIAAARAGKAIFCEKPIDLSLRRVDA
                     CLKVVNQTRVPMFVGFNRRFDPSFRALRERIGKGEIGSVEQVIVSSRDPGLPPISYLK
                     VSGGQFRDMTIHDFDMARWLLGEEPVEVFAYGSCLVDRAVGKIGDTDSAMVLLRTASG
                     RLAHINNSRRATYGYDQRIEVHGSKGRLIAGNHQPTTVQLADERAVSEDKPLYFFLER
                     YAEAYRAELIAFVDAVREGKPMAVTANDGRQAIVLAEAAVKSLRTNRPVKV"
     gene            complement(2565..4007)
                     /locus_tag="VEA63_17010"
     CDS             complement(2565..4007)
                     /locus_tag="VEA63_17010"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HYD85772.1"
                     /translation="MKRFTSLGREAACPEVGLHLKRKLWLLFAAFACATAACGAEKDR
                     HVVVISLDGFPAYLWHRPDLPVPNLRRLAAAGASAEAMTVTNPSSTWSSHTSMITGRS
                     PRYHGVFFNGQVVLKGPRQMPVIEQWADKDGFVLVPTLYDIAHEAGLTTAESDWVAIT
                     RAKTIDWSFPEIPRVDGKVEQEMIAAGLLTAEQIGWMEHKPGRKSLVWHDETWAKAAN
                     FIFEQHRPNLLLFHPLTTDWVHHHYGPGTEASYVALAFADRLVGELVDTVERTGLRDR
                     TTFIVMTDHGFKKVTHSIHANVALRNAGLARSVGPNLVDCDAAVMSLGGTAMVYITDP
                     ARKAELLPKLKALLAATEGIAQVIDGSDGPTLGLPTPQENARMGDLFLVAADGYLFEN
                     SAAAPDVVMPVGNYAATHGYLASDPETDGIFIASGAHIKAGVTLPRVSNLDIAPTLAR
                     LLGLSLPGAEGRVLEKILDEDAAPPAARVR"
     gene            complement(4004..4876)
                     /locus_tag="VEA63_17015"
     CDS             complement(4004..4876)
                     /locus_tag="VEA63_17015"
                     /inference="COORDINATES: protein motif:HMM:NF015000.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HYD85773.1"
                     /translation="MKTKFGRGRARWAWCGLLAVTVTFCFPAAASSSSTYKLIAHRGG
                     VVEDKFPDNSAAALQAAVERGYWGLEVDIRETRDGVLVMQHDPDLRLNYDDPREVKDV
                     TWEEMSRLRSKVANQPPWRFEDVVKAARAAGLWLMLDSKNLHSPDFASKVEAVLKSHG
                     MLERCYVIGASDAMEHFTGKAPVGRKYRSLKPHLDREPAAKEKYFLFDEGTTLTPEMV
                     KWAQSLGVKVVPSINAYHYYDPKTMTGKTRAELAELILPRARQHIEALKALGVREFQI
                     DSEFDGWFEEVKQP"
     gene            complement(4873..>5316)
                     /locus_tag="VEA63_17020"
     CDS             complement(4873..>5316)
                     /locus_tag="VEA63_17020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD85774.1"
                     /translation="VAPWLGETAYTYAITNRFVMTNMVGVPLIMLFFVGTKFFYREPA
                     GSVYERDSAEFFRRMETPVDFEKEVGGDNTAAQAGLLGRIAYLYGAFIVALVLIPNSL
                     GDRVSIFACALIPLAVGLGLRRYARSHTAAHAGPAAAEEPERILP"
BASE COUNT         1011 a         1808 c         1515 g          982 t
ORIGIN      
        1 cgcggaagcc cagcaactga acgtgcagct cgtttcgctc gaagaaattt tcgccacggc
       61 cgacgtcgtc tcctgccaca tgccgctgac cgacgccacg caacgctcgc tcggccgccc
      121 gcatttcgcc gcgatgaaac ccggcgccac gttcatcaac accgcgcgcg gccttctcgt
      181 gcgcgaaaac gaattgatcg acgtgctgaa aacgcggccc gacctccacg ccgtgctcga
      241 tgtgatggag agcgagccgc ctccgccgga gagtccgctg ttctcgctgc caaacgtcgt
      301 catgacgcca cacatcgccg gcagcatcgg cacggaatgc cgccggatgg gcacgatgat
      361 tctcgacgag gtccagcgct acctcgccca cgagccgctt cgcggcgaag tgtggcagga
      421 acagctcaac accctcgcct gactccacct cgtccggaac aattcagttg aaccaaggaa
      481 tgagcacgaa gggacacgaa ggcgaaatca ggaggaaggc gtggttgtag accagtcacc
      541 atccgatcgt ccgatcaccg cagatacttc ggcatgcctc ctctgacatt cgtgtaaatt
      601 cgtgtccatt cgtggttctt cactgcatgg atcccgctct tcccggctta tgagttcgtt
      661 tcgtcattcc gtttcgcaat ggtgcttccc gcacatcccg cttcccgatc tcgcccgcgc
      721 cgcgcgcgac atcggcatcg cctcgatcga attgctcgag cccgccgact ggccggcatt
      781 gcggccgttc ggtctgatat gcgccatggc acgcgggccg ggttcgatcg aggacggttt
      841 taaccggcgc gaaaaccacg ccgcacttgt gccggcgttt cttgaacgca tccgcgagtg
      901 cgccgccgcc ggcgtgccga atgtcgtgtg cttctccggc aatcgacgcg gcctcagcga
      961 cgaagaaggt ctcgatcact gcgccgcggg tttctcgcaa ctcgtcggcg ccgccgagcg
     1021 cgccggagtc acgctctgcc tcgagctgct caacagcaaa atcgaccatc ccgattacca
     1081 atgcgaccac accgcgtggg gcgtcgagct cgtgaagcgt gtcggctcgg agcgtttgaa
     1141 actgctctac gatatttatc acatgcagat catggagggc gacgtggttc gcacgatcga
     1201 acagaaccac gcttacatcg ctcactacca caccgcaggc gttcccggcc ggcacgagat
     1261 cgacgattct caagaactca actatccgcg gatcatgcgc gcgatcgccg cgacgggttt
     1321 caccggcttc gtcgggcagg aatttctccc gctccgcgac ccgctcactt cgctgcgcga
     1381 agccgtcgag ctctgcacgc tcagaccgta gcagcagggc ctccgcgcct gcctgcttgt
     1441 ttgaaaggat aggtcacgcg cttgcgcgtc acatcgcgcc gaacacgcca cgcacaatac
     1501 cacacggcca cgcctcgccg ccttcgcgcc cgcctcacac tttcaccggc cgattcgtgc
     1561 gcagcgattt caccgccgcc tcggcgagca cgatcgcctg acggccgtcg ttcgctgtca
     1621 cggccatcgg cttgccctcg cgcaccgcat cgacaaacgc gatcaactcc gcgcgatacg
     1681 cctcggcata acgctcgagg aaaaaataca gcggcttgtc ctcgctcacc gcacgctcgt
     1741 cggcgagttg caccgtcgtg ggttgatggt tgcccgcgat caaccgccct ttcgaaccat
     1801 gcacttcgat gcgttgatcg taaccatacg ttgcacgccg gctgttgttg atgtgcgcca
     1861 aacgccccga cgcggtccgc aacagcacca tcgccgaatc cgtgtcgccg atttttccga
     1921 ccgcgcgatc gaccaaacag cttccataag cgaacacctc gaccggctcc tcgccgagca
     1981 gccagcgcgc catgtcgaaa tcgtgaatcg tcatgtcgcg gaactgtccg cccgacacct
     2041 tcaggtaaga aatcggcggc aacccgggat cgcgactcga gacgatcacc tgctcgacgc
     2101 tgccgatctc acccttcccg atccgctcgc gcagcgcgcg aaaactcgga tcgaagcgtc
     2161 ggttgaaacc gacgaacatc ggcacgcgcg tttgattcac gaccttcaag cacgcgtcga
     2221 cgcgtcgcag cgaaaggtcg atcggtttct cgcagaaaat cgccttgccc gcccgcgccg
     2281 ccgcgatcgc cagatcggcg tgcgtatcgg tggaggaagc gatgatgacg gcgtccacat
     2341 tgcgatcggc gaaaaccttc gccgcgtccg ccacgcgcgc gccgtgggtg gcggcgagct
     2401 tcgccgcggc gagttcattc acgtcgacga cgtagagcag ttgggcgccg gcagcggcga
     2461 ggttcgaagc gtgaatcgca ccgatgcgac cagcgccaaa ttgagcgaat ttaagcatgg
     2521 gaacttttgg gatgaaattg ggcgaacgtt tgttcgatga ggatctagcg cacccgcgcg
     2581 gccggcggcg ccgcatcttc gtcaaggatt ttctcgagca cgcggccttc ggctcccggc
     2641 agcgacaggc cgagcagccg cgcgagcgtg ggagcgatat cgaggttcga cacacgcggc
     2701 agcgtgacgc cggctttgat gtgcgcaccg cttgcgatga aaattccatc ggtctccgga
     2761 tcgctcgcca gatagccgtg cgtcgccgcg tagttgccga ccggcatcac aacgtccggc
     2821 gccgccgcgc tgttctcgaa caaatagccg tctgcagcga cgaggaataa atcgcccatg
     2881 cgcgcatttt cctgcggcgt cggcaatccg agcgtcggac catcgctgcc gtcgatcacc
     2941 tgcgcgatac cttccgtcgc ggcgagcaac gccttcaatt tcggcagcag ctccgctttg
     3001 cgcgcggggt cggtgatgta gaccatcgcc gtcccgccga gcgacatcac cgccgcgtcg
     3061 caatcgacca gattgggacc caccgagcgc gcgaggccgg cgttgcgcag cgcgacgttg
     3121 gcgtggatgg agtgcgtaac ttttttgaac ccgtgatcgg tcatcacgat aaacgtggtg
     3181 cgatcgcgca gccccgtgcg ttcaacggtg tcgaccaatt cgccgaccag ccggtccgca
     3241 aacgcgagcg ccacataact cgcctccgtc ccgggcccgt aatgatgatg cacccaatcg
     3301 gtggtgagcg gatgaaagag caacaggttc ggccggtgtt gttcgaagat gaaattcgcc
     3361 gctttcgccc aagtctcatc gtgccagacg agcgatttgc gtcccggctt gtgctccatc
     3421 cagccgatct gctccgccgt gagcaggccc gcggcgatca tttcctgttc cactttgccg
     3481 tcgacgcgcg gaatctcggg aaagctccag tcgatcgttt tcgcccgagt gatcgcgacc
     3541 cagtcggact cggccgtcgt caaccccgct tcgtgcgcaa tatcgtagag ggtcggaacg
     3601 agcacaaagc cgtccttgtc cgcccattgc tcgatcaccg gcatttgccg tggccctttc
     3661 agcaccacct ggccgttgaa aaacacgccg tgataccgcg gcgaacgacc ggtgatcatc
     3721 gaggtgtgac tgctccacgt gctcgacgga ttcgtcaccg tcatcgcttc cgcgctcgcg
     3781 cccgccgcgg cgagccggcg gagattcggc accggcaaat cgggccggtg ccaaagatat
     3841 gccggaaatc catcgaggct gataacgacg acgtgccggt ccttttccgc gccacaagcc
     3901 gccgtggcgc atgcaaacgc cgcgaacaaa agccaaagct ttcgcttgag gtggagcccg
     3961 acctccgggc aggcggcttc gcgcccgagt gacgtgaacc gcttcatggc tgtttcacct
     4021 cctcaaacca gccgtcgaac tccgaatcga tttggaattc ccgcacgccg agtgccttca
     4081 acgcttcgat atgctgacgc gcacgcggca gaatcaactc cgccagttcc gcgcgcgttt
     4141 ttcccgtcat cgtcttcgga tcgtagtaat gatacgcatt gatgctcggc acgactttca
     4201 cgccgagcga ctgcgcccat ttcaccatct cgggagtcag cgtcgtgcct tcatcgaaaa
     4261 ggaaatattt ctccttcgcc gccggctcgc gatcgaggtg cggcttcaac gagcgatatt
     4321 ttcgtcccac cggcgccttg cccgtgaagt gctccatcgc gtcactggcg ccgatgacgt
     4381 aacagcgctc gagcataccg tggcttttca gcacagcttc aactttgctc gcgaagtccg
     4441 gcgaatgcag attcttcgaa tcgagcatca accacagccc ggccgcgcgc gccgctttca
     4501 ccacgtcctc gaaacgccag ggcggctgat tcgcgacctt cgagcgcaac cggctcatct
     4561 cctcccaagt cacgtctttc acttcgcgcg gatcgtcgta gttcagccgc aaatccggat
     4621 cgtgctgcat cacgagcacg ccgtcccgcg tctcgcgaat atcgacctcc aacccccaat
     4681 aaccgcgctc gaccgccgcc tgcagcgccg ccgccgagtt gtcgggaaac ttgtcttcga
     4741 ccacgccgcc gcggtgcgct atcagcttat aagtcgacga cgacgacgcc gcggcgggaa
     4801 aacaaaacgt caccgtcact gcgagtagac cgcaccaagc ccaacgcgct cgaccgcgcc
     4861 caaatttcgt tttcatggca gaattctttc cggttcctca gccgccgccg gtccggcgtg
     4921 cgccgccgtg tgactccgcg cgtagcggcg caggccaagt ccaacagcga gcggaatgag
     4981 cgcgcaggcg aagatgctca cgcgatcgcc gagcgagttc gggatgagca cgagcgcgac
     5041 gatgaacgcg ccgtaaagat aggcgatgcg accgagcaga cccgcttgcg ccgcggtgtt
     5101 gtcgccgccg acttccttct cgaagtcgac cggcgtttcc atccggcgga aaaactccgc
     5161 gctgtcgcgc tcgtagacgg agccggccgg ttcgcggtag aaaaatttcg ttccgacgaa
     5221 aaacagcatg atcaacggca cgcccaccat gttcgtcatc acgaagcgat tggtgatcgc
     5281 gtaagtgtaa gccgtttccc cgagccaggg cgcaac
//
LOCUS       DASPLT010001672         1165 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_60263, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001672 DASPLT010000000
VERSION     DASPLT010001672.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1165)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1165)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1165
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_60263"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..918
                     /locus_tag="VEA63_17025"
     CDS             <1..918
                     /locus_tag="VEA63_17025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HYD85775.1"
                     /translation="GLPEMEATHDTWKAGGFRKISPFFIIQLAPNLLAGEVSLLLDFR
                     GPNMSIASACATSGHSLGEAAAAIVRGDADVMIAGGAESTVTPLAIGAFAQMRALSTR
                     NDAPEKASRPYERDRDGFVLSEGAVVFVLEEREQALKRGARIYAELCGYGATADAYHL
                     SSLAPGAEGSARAMRAALARSGLKPTEIDYVSAHATSTPGGDGEEAAAIATVFAENKA
                     NLHVSGVKSMVGHLLGGAGAMGLFAAIKAIETGVIAPTINLENIDPVCADTGLNFTPN
                     TAVRKIVRAALANSFGFGGTNASLVVCAP"
BASE COUNT          192 a          314 c          446 g          213 t
ORIGIN      
        1 ggattgccgg aaatggaggc cacgcacgac acgtggaaag cgggcgggtt taggaagatc
       61 tcgccgtttt tcatcattca actcgcgccg aacctgctcg cgggcgaggt gagcctgctg
      121 ctggattttc gcgggccgaa catgagcatc gcgtcggcgt gcgcgacgag cgggcattcg
      181 ctcggcgagg cggcggcggc gatcgtgcgc ggcgacgcgg atgtgatgat cgcaggcggg
      241 gcggagtcga cggtgacacc gctggcgatc ggcgcgttcg cgcagatgcg ggcgctgtcc
      301 acccgcaacg acgcacccga aaaagcgtcg cggccctacg agcgcgatcg cgacggcttt
      361 gtgctgtcgg aaggcgcggt ggtattcgtg ttggaggaac gcgagcaggc gttgaagcgc
      421 ggcgcgcgga tttacgcgga actgtgcggc tacggcgcga cggcggatgc gtatcatttg
      481 tcgtcgctcg cgccgggcgc ggaaggatcg gcgcgcgcga tgcgggcggc attggcgcgc
      541 tcgggcttga agccgacgga gatcgactac gtgtcggcgc atgcgacgtc gacgccgggc
      601 ggcgacggcg aggaggcggc ggcgatcgcg acggtgtttg ccgagaacaa ggcgaatctc
      661 cacgtgagcg gcgtaaaatc gatggtcggg catttgctcg gcggcgccgg cgcgatggga
      721 ctttttgcag cgataaaagc gatcgagacg ggcgtgattg cgccgacgat caacttggaa
      781 aatatcgatc cggtgtgcgc ggacacggga ctgaatttca cgccgaacac ggcggtgcgc
      841 aaaatcgtgc gcgcggcgct ggcgaacagt ttcggattcg gcggcacgaa tgcgtcgctc
      901 gtggtgtgcg caccgtgagc gacgtggccg gagttgattt gcggacggcc gtcagtcaga
      961 aagtgagcga tggaatcgtc cgcttccgcg cgttgggttg gtgctgtctg tcttcgttgc
     1021 gtcggcggcc gttgatctcg gccatgccat gactcgctgg aaaaaactta ccgcggagtt
     1081 aacggcgcct ttgctcgcgg cgctggcgct gagcggcgga tttttgattt tcgtcgcgtg
     1141 ggatcagtcg cactggtggc gggtg
//
LOCUS       DASPLT010001673         1548 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_285853, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001673 DASPLT010000000
VERSION     DASPLT010001673.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1548)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1548)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1548
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_285853"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(121..>1548)
                     /locus_tag="VEA63_17030"
     CDS             complement(121..>1548)
                     /locus_tag="VEA63_17030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD85776.1"
                     /translation="ETKITAYDFSPFLPLNPFLRRAGMTGLSAEYDCIGEFLGAGFLP
                     AADPQRVIDYVSFAKDQGVHRHVIRVDRIGHPVFTSTQAVNLLAFDRAIADTQVSAAA
                     IWNEWAAAKWPDCASTMTGIMQRGIEAVKKIHFIDGHVIFHAFPIHAEMKWLKACGIF
                     SVFEPEVSLAEHQGMWGILTDRTTPSHAALLHEKDEAVSIADTALAHLRQLRDALPPA
                     EFQLAETAWKNATIVSRLLRAWCRCVCAYFDDMKARRSSHPALTAAIAAGKPEFEQHV
                     PEAFNIPSAVSSAASIPSAHEYGDPEGGSHDIASAYARPLWRLIQLLPVEYQAEWTER
                     SGWITRSDVVDFIVCGGLLDDFRVHRFMHAAHAPLLDGRPARVAGNRVFPNGFIECRL
                     TVPSRGKFLLVVKGAPSKSKGFRLTVNGKTADVLYGENGTFEHLAELPAGVSGVDRFL
                     NIRIQKSGTNYPWIHSIAAVALPGR"
BASE COUNT          307 a          430 c          527 g          284 t
ORIGIN      
        1 ctggtcacga cgatgtggcc ggtcggccac acgttcaacc gggacatgca ggaagcggcg
       61 ttcgactggc tgaacgccgt gctggacccg cgcggcgcac ctgcggagcg gaaaaactaa
      121 tcagcggccg gggagggcga cggccgcgat cgagtgaatc caagggtagt tggtgcccga
      181 tttttggata cgaatattga ggaagcggtc gacgcccgaa acgccggcgg gcagttcggc
      241 gagatgttcg aaagttccgt tttcaccgta gagcacgtcg gcggtttttc cgttcacggt
      301 caggcggaag ccttttgatt tcgacggcgc acccttgacc acgaggagaa atttaccccg
      361 cgacggaacg gtcagacggc attcgataaa gccgtttggg aaaacgcgat taccggcgac
      421 gcgagccggg cggccgtcga gcagaggagc atgagcggcg tgcatgaagc gatgcacgcg
      481 aaagtcgtcc aggagtcctc cgcacacgat gaaatcgacg acatcggaac gagtgatcca
      541 accggagcgc tccgtccatt ccgcttgata ctccacgggc agaagctgga tcagccgcca
      601 gagcggccgg gcatacgcgc tggcgatgtc atggctgccg ccttcgggat cgccgtattc
      661 gtgtgcggac ggaatcgatg ctgcggaaga cacggcggag gggatgttga acgcttcggg
      721 aacgtgttgc tcaaactccg gcttgccggc cgcgatcgcc gcggtgagcg cgggatggct
      781 cgagcgacgg gctttcatgt cgtcgaagta agcgcaaacg caacggcacc aggcgcggag
      841 caaccggctg acaatcgtgg catttttcca cgccgtttcc gcgagttgaa attcggccgg
      901 tggcagcgca tcgcggagct ggcggagatg ggcgagcgcg gtgtcggcga ttgagacggc
      961 ttcgtctttt tcgtggagca gcgccgcgtg cgagggcgtg gtgcgatcgg tgaggatgcc
     1021 ccacatgcct tgatgttcgg cgaggctgac ctcgggttcg aacacggaga atatcccgca
     1081 ggccttgagc catttcattt ccgcatggat gggaaacgcg tgaaagatca cgtggccatc
     1141 gatgaaatgg atttttttca ccgcttcgat tccgcgctgc atgatgcccg tcatcgtgct
     1201 cgcacaatcc ggccacttcg cggcagccca ttcgttccaa atcgcggcgg cgctcacctg
     1261 cgtgtcggcg atggcgcggt cgaaggcgag caggttgacg gcctgcgtgg aggtaaacac
     1321 cggatgaccg atgcggtcca cgcgaatgac gtggcggtga acgccttgat ccttcgcgaa
     1381 cgagacgtag tcgatcacac gttgcggatc ggcggcgggc aggaatcccg cgccgagaaa
     1441 ctcgccgatg cagtcatatt cggcggacaa gccggtcatg cccgcgcgcc gcaggaaagg
     1501 attgagcggc agaaacgggc tgaagtcgta ggctgttatt ttcgtttc
//
LOCUS       DASPLT010001674         1122 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_285923, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001674 DASPLT010000000
VERSION     DASPLT010001674.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1122)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1122)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1122
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_285923"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1122
                     /locus_tag="VEA63_17035"
     CDS             <1..>1122
                     /locus_tag="VEA63_17035"
                     /inference="COORDINATES: protein motif:HMM:NF014850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HYD85777.1"
                     /translation="GPVGAAWPDDLIRWKIELLRDMGANAVRTAHNPQVPRFYELCDE
                     LGLLVLDEVFDGWKQKAPEDYAHQVFGEWWERDLRDWLRRDRNHPSVFLYSLGNETSG
                     EVAAELVRVARETDPTRAYTSGHAAPALMDVYGMNGGSESPRFFRNPPPAKPFIATEA
                     PHTWQVRGFYRTKSWFRDGVNGTGNREPFPLDDLTEDEIFTDDWTAPAAKANRSQVFN
                     SSYDNAMARITARKNWELARDLPWFSGHFRWTGFDYVGEAGFGHGGWPFRAFMGGVID
                     LAGFPKDLYYFYQSQWTTAPMLHLLPHWTHPRMPRGTRVPVWAYTNVDEVELFLNGRS
                     LGRDRPGRQAEEMQCEWFVPWEPGLIEAVGYRDGREVVR"
BASE COUNT          199 a          324 c          378 g          221 t
ORIGIN      
        1 gcgggcccgt cggcgcggca tggccggacg atctgatccg gtggaagatc gaactgctgc
       61 gcgacatggg cgccaacgcg gtccgcactg cgcacaatcc gcaggtgccg cgtttctacg
      121 agttgtgtga cgaactcgga ctgctcgtgc tcgatgaagt gttcgatggc tggaagcaaa
      181 aggcgcccga agattacgct caccaggttt tcggtgagtg gtgggagcgc gacctccgcg
      241 actggttgcg acgcgatcgc aatcatccaa gcgtttttct ctacagcctc ggtaacgaaa
      301 cttcgggcga agtcgcggcc gagttggtgc gggtcgcacg cgagaccgat ccgacgcgcg
      361 cctacacctc gggtcatgcg gcgccggcgt tgatggatgt ttacgggatg aacggcggtt
      421 ccgagtcgcc gcggtttttc cgcaatccgc cgccggcgaa accgttcatc gccaccgagg
      481 cgccgcacac ctggcaggtg cgtggatttt atcggacgaa aagctggttt cgcgatggcg
      541 tgaacggcac cggcaaccgc gagccgttcc cgctggacga tttgaccgag gacgaaatct
      601 tcacggatga ctggacggca cccgcggcaa aagcgaatcg cagccaggtg ttcaattcgt
      661 cctacgacaa tgccatggcg cgcatcactg cgcggaaaaa ttgggagctc gcgcgcgatc
      721 ttccatggtt cagcgggcat ttccgctgga ctggtttcga ttatgtcggc gaggcaggct
      781 tcggtcatgg tggctggccg ttccgcgcct tcatgggcgg cgtgatcgac ctcgctgggt
      841 ttcccaagga tctctattat ttctaccaga gccaatggac gacggcgccg atgctgcacc
      901 tgctgccgca ctggacgcat ccgcgtatgc cgcgtggcac gcgtgttccc gtgtgggcct
      961 acacgaacgt cgacgaggtg gaactcttcc tcaacggacg ttcgctgggt cgcgaccgtc
     1021 cgggccggca ggcggaggaa atgcaatgcg agtggttcgt gccatgggag ccgggtttga
     1081 tcgaggcggt aggttatcgc gacggacgcg aagtggtgcg tg
//
LOCUS       DASPLT010001675         2268 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_122603, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001675 DASPLT010000000
VERSION     DASPLT010001675.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 2268)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2268)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2268
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_122603"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(87..1040)
                     /locus_tag="VEA63_17040"
     CDS             complement(87..1040)
                     /locus_tag="VEA63_17040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HYD85778.1"
                     /translation="MTKPQTFRNLIKTAGLASLFAFAASAFAQDGTIYPLDAPPEPNA
                     IPLNTGGVENQPAEESWFRQWGEPMVRNVSTATLTPFLPDPAKANGTAVIVAPGGGFR
                     WLSINNEGWKIAKALNEQGVAAFVLKYRLNPTPPTLEGLRESMNRTFATVTPAAGAPA
                     AEAPRPPRFNLENQLADAEAAYAMIVARAKEWNVDTNRIGMVGFSAGAGLTMHSTLNS
                     KTMKLAFIAPIYGGMGAVEVPKDAPPMFVAIAVDDFLFRGDFGLIKSWYDAGRPVEFH
                     LYQHGGHGFGMGYPDHPTYWWFEVFTHWLDVNGFLKAKAAE"
     gene            complement(1080..1934)
                     /locus_tag="VEA63_17045"
     CDS             complement(1080..1934)
                     /locus_tag="VEA63_17045"
                     /inference="COORDINATES: protein motif:HMM:NF015585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HYD85779.1"
                     /translation="MSRSCLSFFFASSLLLSAVAHAQDENFHIFLCLGQSNMEGFPGI
                     PDEEKTYDEPRFQVLAAVDFSALGREQGRWYPAVPPLCRPDSGMSPADYFGRTLVAAL
                     PARVRVGVVNVSVAGCKIDLFEQTTFPDYVASAPAWMQGALADYGGNPYQRLLDMARV
                     AQRSGVIKGILLHQGESNVGDSAWPAKVKAVYERLLADLGLPAAEVPLLVGGLVTAEE
                     GGKCGSMNPIIADLPTTIPTAHFVSSEGCEAVPDGLHFSPAGYRELGRRYAETMLPLL
                     GFPAHGPQ"
     gene            complement(1931..>2268)
                     /locus_tag="VEA63_17050"
     CDS             complement(1931..>2268)
                     /locus_tag="VEA63_17050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD85780.1"
                     /translation="PDIEVYYYARGPAKNLEVISYGFDPETKMNWPLEWTVAYGRGRV
                     YTSTFGHVWKGDTQPERMRCAGLHTVVLRALQWLADRTPDYPVPADFPTAEKLSVRGK
                     IALPTAPSL"
BASE COUNT          434 a          701 c          727 g          406 t
ORIGIN      
        1 atccgtgttc atcggtgtcc atccgtggtt ttccgatcgg gcgacgcgtg acttcgcgac
       61 ggaaatcgta gaatctggaa gcggtgtcac tcggccgctt tggccttgag gaaaccgttc
      121 acgtcgagcc agtgcgtgaa cacttcgaac caccagtagg tcgggtgatc cgggtaaccc
      181 atgccgaagc cgtgtccgcc gtgttgatac aggtgaaact cgaccggacg gccggcgtca
      241 taccacgact tgatcaaacc gaagtcgccg cggaaaagaa agtcgtcgac cgcgatcgcc
      301 acgaacatcg gcggcgcatc cttcggcacc tcgaccgcac ccatgccgcc gtagatcggc
      361 gcgatgaacg cgagcttcat ggtcttcgag ttcagcgtgg agtgcatcgt gagacccgcg
      421 ccggcggaaa atccgaccat gccgatgcgg ttggtatcga cgttccattc cttcgcgcgc
      481 gcgacgatca tcgcgtaggc cgcttcggcg tcggcgagct ggttctcgag attgaagcgc
      541 ggcggacgcg gcgcttcggc agcaggagcg ccggcggctg gagtaacggt ggcgaacgtg
      601 cggttcatcg actcgcgcag gccttcgagc gtcggcggcg tcggattcag acgatacttc
      661 aggacgaagg cggcgacgcc ttgctcattc agcgccttcg caattttcca accctcgttg
      721 ttgatcgaga gccagcgaaa gccgccgcca ggcgcgacga tgacggccgt gccgttggct
      781 ttggccgggt ccggcagaaa tggcgtcagc gtcgcagtcg aaacgttgcg caccatcggc
      841 tcgccccact ggcggaacca gctctcctcc gccggctggt tttcgactcc gcccgtgtta
      901 agcggaatgg cgttgggttc cggcggagcg tcgagcggat aaatggtgcc gtcctgcgcg
      961 aaggcggatg cggcgaacgc gaacagcgag gcgaggcccg ctgttttgat gaggttcctg
     1021 aaagtttggg gcttggtcat gtttgggtgt tggttgggga acgtatcgtc tgacaaaagt
     1081 cactgggggc cgtgcgcggg aaacccgaga agcggcaaca tcgtttcggc gtagcggcgg
     1141 ccaagttcgc ggtaacccgc cgggctgaaa tgcaggccgt ccggcacggc ttcgcagcct
     1201 tccgaggaga cgaaatgcgc ggtcggaatc gtcgtcggca gatcggcgat aatcggattc
     1261 atgctgccgc atttgccgcc ttcttccgcg gtaaccagac caccgacaag caacggcacc
     1321 tcggcggccg gcaaaccgag atcggcgagc aggcgttcgt agacggcctt caccttcgcc
     1381 ggccacgccg aatcgccgac gttcgactcg ccctgatgaa gcaggatgcc cttgatgacg
     1441 cccgatcgct gagcgacccg cgccatgtcc aggagccgtt ggtaaggatt gccaccgtag
     1501 tccgcgagag cgccttgcat ccatgccggc gcgctagcca cgtagtcggg aaacgtcgtt
     1561 tgctcgaaaa ggtcgatctt acatccagcg accgaaacgt tcaccacgcc gactctcacg
     1621 cgcgcgggga gcgccgccac gagcgtgcga ccaaagtaat cggcggggct catgccggag
     1681 tccggccggc agagcggagg caccgccgga taccatctgc cctgctcgcg cccgagcgcg
     1741 gaaaaatcca ccgccgccag cacctggaaa cgcggctcat cgtaagtctt ctcctcgtcc
     1801 gggatgccgg gaaacccttc catgttcgac tgtccgagac agaggaaaat gtggaaattc
     1861 tcatcctgcg cgtgcgcgac ggcgctcagc aggagcgagc ttgcgaagaa aaacgacagg
     1921 cacgatcgac tcataaactc ggtgcggtgg gcagcgcgat tttgcctcgc acggagagtt
     1981 tctccgcggt cggaaaatcc gccggcaccg gatagtcggg tgttcggtcg gcgagccact
     2041 gcaacgctcg caagacgacc gtgtgcaagc cggcgcaacg catgcgctcc ggctgcgtgt
     2101 cgcccttcca aacgtgtccg aaggtggagg tgtaaacgcg tccacgcccg taagcgacgg
     2161 tccactcgag cggccagttc atcttcgtct cgggatcgaa cccgtaagat atcacctcga
     2221 gattcttcgc cggaccgcgc gcgtaataat atacctcgat gtccggcg
//
LOCUS       DASPLT010001676         1834 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_81447, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001676 DASPLT010000000
VERSION     DASPLT010001676.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1834)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1834)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1834
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_81447"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..237)
                     /locus_tag="VEA63_17055"
     CDS             complement(<1..237)
                     /locus_tag="VEA63_17055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD85781.1"
                     /translation="MASIKMLLHRQEILPLQRDELRRARLDRRKRGVNRTQAIHHFNR
                     QAVRTTHGNKIHSAAHFPVRQFPSCESDRLDERFH"
     gene            complement(258..584)
                     /locus_tag="VEA63_17060"
     CDS             complement(258..584)
                     /locus_tag="VEA63_17060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD85782.1"
                     /translation="MLPDFASNAGFDRIVLNAANAPFPLSLITHPTIKPLTLPKRSRW
                     TEHSIDRPCRPSFDPAHDLTKSSRAIWPGFEQGVPVIRHDRIVRDLNVPFRQRVQFRH
                     HHLGRT"
     gene            complement(618..1613)
                     /locus_tag="VEA63_17065"
     CDS             complement(618..1613)
                     /locus_tag="VEA63_17065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HYD85783.1"
                     /translation="MSEDLCLSCIRDLKSSDTAGNGRLFTSLLVVKKLAAKTASNGNA
                     FFSAELGDRTGSCSCTIFSDSPVFETLKAAGEGAVVRVEGRVDFYQGRLSPKLSRVTV
                     LSEEELGAPGLLDNLVETAPENPDALWAEINTFIESIVHAELRATVRAVFDDIGDVFR
                     WAPAAVSMHHAYRHGLLEHTVHMARACRALLPLYPEVHADLAMAGILLHDTGKTIEYE
                     GTLATKRSRRGILQGHVVLGYQLARKAAMKSKLPADLLERLEHIILSHQGEPEWGAAI
                     YAATPEAVFVSMIDNLDAKMGMVQRALRQASDDTEFSERLPGLSSPLLTKPIPPQ"
BASE COUNT          344 a          455 c          678 g          357 t
ORIGIN      
        1 gtgaaatctc tcgtccaaac gatccgattc acacgaggga aactgccgca ctgggaagtg
       61 cgcggcggag tgtattttgt taccgtgcgt tgtgcggaca gcttgccgat tgaagtggtg
      121 gatcgcctgc gtgcggttca cgccacgctt gcgacgatcg agccgagctc ttcgcaattc
      181 gtcgcgctgc aacggcaata tttcctgacg atggagaagc atcttgatgc tggccatgga
      241 caatgccgat tggggactca cgtgcggccg aggtggtggt ggcggaactg cacgcgctgg
      301 cggaatggca cgttaaggtc cctcactatt cgatcatgcc gaatcactgg cacgccttgc
      361 tcgaacccgg gccagattgc acgcgagctc ttggtgagat catgcgcagg gtcaaaggac
      421 ggacggcacg gacgatcaat gctgtgttcg gtccagcggg agcgttttgg cagcgtgagt
      481 ggtttgatcg ttggatgcgt aatgagggag agcgggaacg gtgcgttcgc tgcattcaaa
      541 acaatccggt caaagccggc attacttgcg aagtcgggga gcatccgtgg acacgataag
      601 gcgggctgaa gcccgcccta ctggggcgga atgggctttg tcagcagggg ggagctgagg
      661 ccggggaggc gttcggagaa ctcggtgtcg tcgctcgcct ggcggagggc gcgttggacc
      721 atgcccattt tggcgtcgag gttgtcgatc atcgaaacga aaacggcttc gggggtcgca
      781 gcgtagatgg cggcgcccca ctcgggttcg ccttggtgcg aaagaatgat gtgctcgagg
      841 cgttcgagga gatcggcggg gagcttcgat ttcatcgcgg ccttgcgcgc gagttgataa
      901 ccgaggacga cgtggccttg gaggatgccg cggcggctgc gtttggtcgc gagcgtgcct
      961 tcgtattcga tcgtcttgcc cgtatcgtga agtaggatac cggccatcgc caggtcggcg
     1021 tggacttcgg ggtagagcgg aaggagggcg cggcaggcgc gcgccatgtg caccgtgtgt
     1081 tcgagcaaac cgtggcggta tgcgtggtgc atcgagacgg cggcgggcgc ccagcggaaa
     1141 acgtcgccga tgtcgtcgaa cacggcgcga acggtcgcgc gcagttcggc gtgaacgatg
     1201 ctctcgatga aggtgttgat ctcggcccag agcgcgtcgg ggttttcggg cgcggtctcg
     1261 acgaggttgt cgagaagacc gggggcaccc agctcctctt cggagaggac ggtgacgcgc
     1321 gagagtttcg gcgataggcg gccctgataa aaatcgacgc ggccttcgac gcggacaacg
     1381 gcgccttcgc cggcggcttt gagggtttcg aaaacggggc tgtcgctaaa aatggtgcaa
     1441 gagcaggaac cggtgcgatc gccgagctcg gcgctgaaaa acgcgttgcc gttcgaggcg
     1501 gttttggcgg cgagtttctt tacgacgagg aggctcgtga agagccggcc gttaccggcc
     1561 gtgtcggagg atttgagatc gcgaatacag gagaggcaga ggtcttcgga catgggacgc
     1621 gtttcgcgca cgttgcgcgg ggaacgggcc ggcgacaagg ctgcggtttc gggcgagtgt
     1681 tgcgtgcgag cgcagaacct acatgggcgc gaggtcgcga ggaggctgat gttacgcgtg
     1741 ggtttttacg agctgcgtgt aggatttgaa gaacggatga gcgacgtcgt gcagcaaggc
     1801 gtagaaaacg gattcgcttg gcagaacgtg aacg
//
LOCUS       DASPLT010001677         1517 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_60460, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001677 DASPLT010000000
VERSION     DASPLT010001677.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1517)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1517)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1517
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_60460"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(226..546)
                     /locus_tag="VEA63_17070"
     CDS             complement(226..546)
                     /locus_tag="VEA63_17070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007361275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD85784.1"
                     /translation="MVKGQKVVCINDTFTPVILALYKQLPVKGDVYTIREVFLGREKI
                     VRGGDSATVGLLLEELKNPPDPFHQGKQELGFSSERFAPLEELPAEEVEAEEVVGVGA
                     WAPA"
     gene            complement(577..990)
                     /locus_tag="VEA63_17075"
     CDS             complement(577..990)
                     /locus_tag="VEA63_17075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009509990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD85785.1"
                     /translation="MRELFSAKAAVVHRYAWLLETLETEPTLFVRSMFGGKAVYLHGR
                     LVLSLNAHEEPWRGVLVATSQEHHASLVAECAALRVHPILGKWLYLPEASATFERDAT
                     WLVARIRACDARIGVEPGKKIRKSRSRPTQSRKRG"
     gene            complement(1019..1516)
                     /locus_tag="VEA63_17080"
     CDS             complement(1019..1516)
                     /locus_tag="VEA63_17080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007361276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5069 domain-containing protein"
                     /protein_id="HYD85786.1"
                     /translation="MTCRKPPRQSGAMPSKPIPGSSGDVLTCLPSPYIAHAATGLLYL
                     PRFLAKCRYVKEHGALPPSYAKNYKRGIDRFLSLHLGIDPAAVEKIVFESSSDEEVEA
                     KLKTLLPGDVRAAKWNRELAQKGMTDAGQEFIKIALTAMGCPDRVGEIRSVPDLIDFD
                     EGRIE"
BASE COUNT          289 a          428 c          517 g          283 t
ORIGIN      
        1 agacgaggcg gcagtggcga gactggagga catgatcgca gcgacgacgg tcgcgaggag
       61 gaggagtttt tgcatgggga atggtgacga aagacgatgc ggcgggacgg aagatgcgtc
      121 cgacggcatc ggacgcggtg gttcgctgcg tgagcagcgg gaggttgaac gatatcgaag
      181 ccgtaacatc cctgcgctta cgcgcagagc cacgcaatca tgcaatcacg ccggcgccca
      241 cgcgccgacg ccgacgacct cctcggcctc gacttcctcg gcgggaagtt cttcgagcgg
      301 cgcgaaacgt tcggaggaaa agccgagctc ttgttttccc tgatgaaagg gatcgggcgg
      361 atttttcagc tcttcgagca gcagaccaac cgtggcgctg tcgccgccgc ggacgatttt
      421 ttcgcggccg agaaagacct cgcggatcgt atagacgtca cccttcaccg ggagctgctt
      481 gtagagcgcc aggatgacgg gagtgaacgt atcgttgatg cagacgactt tctggccttt
      541 gaccatgcga gcagtgtgag aggagcgggc cgcgattcaa ccacgcttgc gggattgcgt
      601 cggacgtgag cgcgacttgc gaattttttt cccgggttcg acgccgatgc gcgcgtcgca
      661 ggcgcgaatt cgcgcgacca gccacgtcgc gtcgcgctcg aaggtcgcgc tggcttccgg
      721 caggtaaagc cacttaccga gaatcggatg cacacggagt gcagcgcatt cggcgacgag
      781 cgaagcgtgg tgttcctgcg acgtggcgac gagcacgccg cgccagggtt cctcgtgggc
      841 gttgaggctg aggacaagcc ggccgtgcaa ataaacggct ttgccgccga acatcgagcg
      901 aacgaagagc gtcggctccg tctcgagcgt ttcgagcaac catgcgtagc ggtgaacgac
      961 ggcagctttg gcggagaaga gctcgcgcat atggccgttc ccggttgaac gcccgatcct
     1021 actcgatgcg gccttcgtcg aaatcgatga ggtcgggcac gctcctgatt tcgccgacgc
     1081 gatcggggca acccatcgcg gtcagcgcga ttttgatgaa ttcctggccg gcgtcggtca
     1141 tgcctttttg cgcgagctcg cgattccatt ttgcagcgcg cacgtcgccc gggagcagcg
     1201 ttttcaattt ggcttcgacc tcctcgtcgg aagacgactc gaagacgatt ttctcgacgg
     1261 cggccggatc gatgccgaga tgcagcgaga gaaaccgatc gatgccgcgt ttgtagtttt
     1321 tcgcgtagct tggcggcagc gcgccgtgct ctttcacgta gcggcacttc gcgaggaaac
     1381 gcggcaggta aagcaaaccg gtcgcggcgt gcgcgatgta aggcgacggg aggcacgtga
     1441 gaacgtcgcc cgacgagccg ggaatgggct tcgatggcat cgcgccggat tgtcgtggcg
     1501 gtttccggca ggtcaac
//
LOCUS       DASPLT010001678         2344 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_60505, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001678 DASPLT010000000
VERSION     DASPLT010001678.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 2344)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2344)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2344
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_60505"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2344
                     /locus_tag="VEA63_17085"
     CDS             <1..>2344
                     /locus_tag="VEA63_17085"
                     /inference="COORDINATES: protein motif:HMM:NF024728.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemagglutinin repeat-containing protein"
                     /protein_id="HYD85787.1"
                     /translation="LNATAGNIHNDNNGAIYAAGNLSLTATGNIINDEDATIDGDADF
                     TASGTGLTSRFTNQGQINIGGNATINARNFENVMAGAENLVREWRGLNTTGSSINQHN
                     YTTANETVPDVSTTSWYYDGHGGYSGDWFDDRESAVYGHFYRKEFFQGIADAEATFGD
                     KTKPQISATNLTVSGFDSGRNVGGLMTATDTLTISGASGSSTFLNDSMALGLEKWNYQ
                     ATWWYEYTTGSYDWWNPTGGNHITNRLTTLDSAQSYFSIGAGIRAGTLIVANVGALTN
                     MGSPFGPATDALDDQGEAFEIRDTGAAGTNLSDAGETTTVGLGSASMVNFTSRSTVAG
                     AATTKGATLLSSENAKTSGAGKSNSLKSSDEATGGAADRFTSRNAAAFANALKNAKIS
                     LNGLTLNIPSNPNSYFVSNADPKAKYLVNVNASFGIDPGSAVGSDELFKQLGADPDNV
                     QKRLGDAAYETYLVRQQLVDQLGTKFLDGQKNEADQMAGLMANAATEARRLGLQIGVA
                     PSPAQLANLTEDMVWMVEQEVAGERVLVPQVYLSSKTRGLFDAGTATLAGDKVEMDVG
                     SLANTGGTISGSDSLSIKAKDDITNLSGNISGGDVSLNAGGSIINETTTRGNADSTVV
                     GRTAGITATGDLDLDAGKDIKIIGGDVKAAGDASIAAGGEIVVDTVQKKTTTRTTEAS
                     SGFIGSSKTTTTTVDVENQASNLDFGGNVKIKSGGDTTIAGSNLNVGGNLDADVGGNL
                     NVLARQDQHTVNTESSTSGVGVGGGIYGSQTTTTNDFTGTN"
BASE COUNT          561 a          776 c          645 g          362 t
ORIGIN      
        1 ctcaacgcga ccgctggcaa tattcacaac gacaacaacg gcgccatcta cgccgccgga
       61 aatctcagcc tcaccgcgac cggcaacatc atcaacgacg aggacgccac catcgacggc
      121 gacgccgatt tcaccgcctc gggaacgggc ctcacctcac gcttcaccaa ccagggtcag
      181 atcaacatcg gtggcaacgc cacgatcaac gcgcgcaact tcgaaaacgt catggccggc
      241 gcggaaaacc tcgttcgcga atggcggggc ctcaacacaa ccggctccag catcaatcag
      301 cacaactaca cgaccgcgaa cgaaactgtg cccgacgtct ccaccacaag ttggtattac
      361 gacggacacg gcggctacag cggcgattgg ttcgacgacc gcgaatcggc ggtttacggg
      421 catttctatc gcaaggaatt cttccagggg atcgccgacg cagaagcgac ttttggcgac
      481 aaaaccaaac cgcagatctc cgcgacgaat ttgaccgtga gcggattcga ctccgggcgg
      541 aatgtcggcg ggttgatgac tgcgacggac accctgacga ttagcggtgc cagcggcagc
      601 tcgacttttc tcaacgattc gatggcgctg ggcctcgaga agtggaacta ccaggcgacc
      661 tggtggtatg agtataccac cggcagttac gactggtgga atccgaccgg cggcaaccac
      721 atcaccaatc gactcacgac actcgattcc gcccaaagtt atttcagcat cggcgcggga
      781 atccgcgctg gcaccctgat cgtcgccaac gtcggagcac tgacgaacat gggttcgccc
      841 ttcggtccgg caaccgacgc ccttgatgat caaggagagg ccttcgaaat ccgcgacacg
      901 ggagccgcag gcacaaactt gtcggacgcg ggggagacca ccaccgtcgg attaggcagc
      961 gccagcatgg tcaacttcac gagccgctca accgtagcgg gcgcagcgac caccaaaggc
     1021 gcgaccctgc tttcgagtga aaatgccaaa acgagcggtg caggaaagag caactccctc
     1081 aaaagcagcg acgaagccac cggcggtgcc gccgatcgct tcacctcccg aaacgccgcc
     1141 gcttttgcca acgcgctcaa gaacgcgaag atctcgctca acggcctcac cctcaatata
     1201 ccgtcgaatc cgaacagcta cttcgtttcg aacgccgatc cgaaggccaa gtatctcgtc
     1261 aacgttaacg cctccttcgg catcgatcca ggcagcgccg tcggttccga cgaactcttc
     1321 aagcagctcg gcgccgatcc ggacaacgtt caaaaacgtc tcggcgacgc cgcatatgag
     1381 acctacctcg tccgccaaca gctcgtcgac caactcggaa cgaagttcct cgatggccag
     1441 aaaaacgaag ctgatcaaat ggcaggcctc atggccaacg ccgcgacgga agcgcgtcgc
     1501 ctcggcctcc agatcggcgt cgcgccctcg cccgcccaac tcgccaatct caccgaagac
     1561 atggtgtgga tggtcgagca ggaagtcgct ggcgagcgcg tgctcgttcc ccaggtttat
     1621 ctttcatcga aaacacgcgg tctttttgat gccggcaccg ctacgctcgc cggcgacaag
     1681 gttgagatgg atgtcggcag cctggccaac accggcggca cgatttccgg gtcggattcg
     1741 ctcagcatca aggccaagga cgacatcacc aacctcagcg ggaatatctc cggcggcgac
     1801 gtctctctga acgccggtgg aagcatcatc aatgaaacca ccactcgcgg caacgccgat
     1861 agcacggttg tcggtcgcac cgcgggcatc accgccaccg gcgatctcga tctcgatgcg
     1921 ggcaaggaca tcaaaatcat cggcggcgac gtgaaagccg cgggcgacgc ttcgatcgcg
     1981 gcgggcggcg agatcgtcgt cgacaccgtc cagaaaaaga ccacgacccg cacgaccgaa
     2041 gcatcgagcg gcttcattgg aagcagcaaa accaccacga ccacggtcga tgtggaaaac
     2101 caagcctcga atctcgattt cggcggcaac gtgaaaatca agagcggcgg cgacaccacg
     2161 atcgccggca gcaatctgaa cgtcggcggc aacctcgacg ccgatgttgg cggcaacctc
     2221 aacgttctcg cgcgccagga tcagcacacc gtcaacaccg agtcctctac aagcggcgtc
     2281 ggtgtcggcg gcggcatcta cggctcgcaa acaacgacca cgaacgattt caccggcacg
     2341 aacg
//
LOCUS       DASPLT010001679         1319 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_122755, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001679 DASPLT010000000
VERSION     DASPLT010001679.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1319)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1319)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1319
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_122755"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1319)
                     /locus_tag="VEA63_17090"
     CDS             complement(<1..>1319)
                     /locus_tag="VEA63_17090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HYD85788.1"
                     /translation="MRDKYGIGSVYYAHAGAGELHTRPLFNLRTEEGLKMFRGIATDV
                     AALVKKYRGSLSGEHGDGRLRGEFIRFMVGDACYAMMRRVKEAFDPFNLFNPGKIIDT
                     PPMDTSLRQGPGQPDPHYRTLFDFSAAGGVLHATEKCTGVGECRKSHLMGGTMCPSYM
                     ATRNEQDTTRARANMLREVLTNPRNVRNAWDSAEVKSVMDLCLSCKGCKSECPSNVDV
                     ARLKAEWQQHYLDEHGVPFRSRLVAGFAPAMRLASIAPALYNWAVTNGVVSRWIKRIS
                     GFAVERSLPTLHRTTLAAWHARHANPRSLSYPNGRVHLFCDEFTNYNDTGIGIKAVQL
                     LNRLGYEVVIPPHVESGRAHFSKGLVREARKLAVRNVELLKDVVTSEAPLIGIEPSAI
                     LGFRDEYPDLVPPHLKEAAQQLAKNALLIDEFIAREAEKGRIPRSAF"
BASE COUNT          236 a          447 c          387 g          249 t
ORIGIN      
        1 ttgaacgcgg agcgcggaat gcgccctttt tccgcttcgc gtgcgatgaa ctcatcgatc
       61 agcagcgcgt tcttcgcgag ctgttgcgcg gcctctttta aatgcggcgg cacgaggtcc
      121 ggatactcgt cgcgaaagcc aagaatcgcc gacggctcga tcccgatgag cggcgcctcg
      181 ctcgtgacga cgtctttcaa gagctcgacg ttgcgaacgg cgagcttccg cgcctcgcgc
      241 accagccctt tggagaaatg tgcgcgcccg ctctcgacgt gcggcggtat cacgacctcg
      301 tagccgagcc ggttcaagag ctgcaccgcc ttgatgccga tgccggtgtc gttgtagttc
      361 gtgaactcgt cgcaaaaaag atgcacgcga ccattcgggt aagagagcga acgcggattc
      421 gcgtgtcgcg catgccacgc ggcgagcgtc gttcgatgca acgtcggcaa actccgttcc
      481 accgcgaagc cggaaatccg tttaatccat cgcgagacca cgccgttggt caccgcccag
      541 ttgtagagcg caggcgcgat cgacgcgaga cgcatggccg gcgcaaagcc cgcgaccagc
      601 cgcgagcgaa acggcacgcc gtgctcgtcg aggtaatgct gctgccactc ggctttcagc
      661 cgcgcgacgt cgacgttcga cggacactcg gacttgcagc ctttgcacga gagacaaagg
      721 tccatcacgc ttttcacctc ggcgctgtcc cacgcgttgc gcacattgcg cggattcgtc
      781 agcacctcgc ggagcatgtt cgcccgcgcg cgcgtcgtgt cctgctcgtt gcgcgtcgcc
      841 atgtaactcg ggcacatcgt gccacccatg agatgcgatt tgcgacactc gccgacgccc
      901 gtgcactttt ccgtcgcgtg caacacccct cccgccgcgc taaaatcgaa caacgttctg
      961 tagtgcggat ccggctgccc cggcccctgc cgcagcgaag tgtccatcgg cggcgtgtcg
     1021 atgatcttgc ccggattgaa aaggttgaac ggatcgaacg cctccttcac ccgccgcatc
     1081 atcgcgtagc aggcgtcgcc gaccatgaag cgaatgaact cgccccgcag ccgtccgtcg
     1141 ccgtgctcgc ctgagagcga accgcgatac ttcttcacca acgcggccac atccgtcgcg
     1201 atgccgcgga acatcttcaa cccttcctcc gttcgcagat tgaacagcgg ccgcgtgtgc
     1261 agttcgcccg cgccggcatg tgcgtagtag acgctgccga tgccgtattt gtcgcgcat
//
LOCUS       DASPLT010001680         1225 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_286106, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001680 DASPLT010000000
VERSION     DASPLT010001680.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1225)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1225)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1225
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_286106"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..762)
                     /locus_tag="VEA63_17095"
     CDS             complement(<1..762)
                     /locus_tag="VEA63_17095"
                     /inference="COORDINATES: protein motif:HMM:NF013300.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004560 - alpha-L-fucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HYD85789.1"
                     /translation="MKRTTLVAALGLLTWLPASAQPSGDTRDLRLPATDAHLRQVKDY
                     IEAKPDADYRHASPAAHEAFRDMKFGVRIHWGLYSMWALQEESWPFLKMSNERRQEFQ
                     QLPSQWNPQGFDAEEWMSFFERAGMKCFAFTTKHHEGFSLFDTKTRVRQRVNWTAPGG
                     PQIEACDVAYSVMETPFRRDIVAELCAAARRHDLKIDLYFSHPDWYDADFRPYIYHPL
                     QTPHTRELARTDPGTLKRLHGHPPTIVPDPSPEETA"
     gene            complement(813..>1225)
                     /locus_tag="VEA63_17100"
     CDS             complement(813..>1225)
                     /locus_tag="VEA63_17100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007363504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HYD85790.1"
                     /translation="PALMERHDNITQRLLARDELTACFIPDGVHLPAFVLRNFFRAKP
                     RGRTLFTTDAMAGAGAPPGRYTLGALEIDIGDDRVARQPGREGFAGSTLTPRDGVTRV
                     MEYLGLSFDEALNLWSDASAAAFGLPPLPDNEEN"
BASE COUNT          228 a          364 c          411 g          222 t
ORIGIN      
        1 cgccgtttcc tcgggggatg gatcgggcac gatcgtcggc ggatgaccgt gcaaacgctt
       61 cagtgtgccg ggatccgtgc gcgccagttc gcgcgtgtgc ggcgtctgga ggggatgata
      121 aatgtagggc cgaaagtcgg cgtcatacca atctggatgc gaaaaataga gatcgatctt
      181 gaggtcgtgt cggcgcgctg cggcgcacag ctcggccacg atatcgcggc gaaaaggcgt
      241 ctccatcacg ctgtaggcga catcgcaggc ctcgatttgc ggaccgccgg gcgcggtcca
      301 gttcacccgc tgccgcacgc gcgtcttggt gtcgaagagg gagaatccct cgtgatgttt
      361 ggtggtgaac gcgaagcact tcatccccgc gcgttcgaaa aacgacatcc actcctcggc
      421 gtcgaagccc tgcgggttcc actgtgaagg gagttgctga aactcctgcc ggcgttcgtt
      481 cgacatcttg agaaagggcc aggactcctc ttgcaatgcc cacatggaat acagacccca
      541 gtgaatccga acgccgaact tcatgtcgcg aaacgcctcg tgcgcggccg gcgatgcgtg
      601 tcgataatcc gcgtccggtt tcgcttcgat gtagtctttg acctgccgta ggtgagcgtc
      661 ggtggccggc agacgaagat cgcgcgtgtc gccactgggc tgcgccgagg cgggcaacca
      721 tgtcagcagc cccagggccg cgacgagagt ggttcgcttc acgggccgaa ttattttcga
      781 gcgcatccag aaagcagggc tatgcgacgt gcttaatttt cctcgttgtc cggcagcggc
      841 ggcaggccga aggcggcggc ggacgcgtct gaccagaggt tcaaagcttc gtcgaaggac
      901 aaaccaagat attccattac gcgcgtgaca ccgtcacgcg gagtgagcgt cgatcccgca
      961 aaaccctcgc gcccgggctg gcgcgcgacg cgatcgtcgc cgatgtcgat ctccagcgcg
     1021 cctaacgtat accggcccgg cggggcgcca gcgccggcca ttgcgtcggt ggtgaagagc
     1081 gtgcgaccgc ggggcttggc gcgaaagaaa ttgcgcaaca cgaatgccgg cagatgcacg
     1141 ccgtcgggga tgaagcaggc cgtgagttcg tcgcgcgcga gcaggcgctg cgtgatgttg
     1201 tcgtgacgct ccatcagcgc cggcg
//
LOCUS       DASPLT010001681         1021 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_81639, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001681 DASPLT010000000
VERSION     DASPLT010001681.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1021)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1021)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1021
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_81639"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..439)
                     /locus_tag="VEA63_17105"
     CDS             complement(<1..439)
                     /locus_tag="VEA63_17105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD85791.1"
                     /translation="MARWTTIALFTALPLWAAESIDELHERATTAHRQRNIGEASAFY
                     ARMLAAEPPAKPSENERALVRQFAPRLHLVAGEFFPLKDIVAIVHPDRPVIAYHLFWD
                     DDIGYPADNEPCDHEIVWVEYDPATRVIVQVWTYFHGRVVSTPV"
     gene            complement(460..>1021)
                     /locus_tag="VEA63_17110"
     CDS             complement(460..>1021)
                     /locus_tag="VEA63_17110"
                     /inference="COORDINATES: protein motif:HMM:NF019552.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="heparinase II/III family protein"
                     /protein_id="HYD85792.1"
                     /translation="SGRWRYYRLNNLSHNTVTPGEALQSPDATAAIVRYASTPERAFA
                     IADLSEAYPEMATSHRRGIAMLDRARVLVQDEISGVRAQTPLTWRMLTGTDVNVSGRV
                     ATLTHDGRMLRAEIISPANVFWTSHPAKPPTAAENQNDGITALEAVVPAVQTAADVNI
                     AVLLTPVGERWPSRPVPQLRPLDEWK"
BASE COUNT          186 a          339 c          304 g          192 t
ORIGIN      
        1 cgaccggcgt gctgaccacg cggccgtgaa aatacgtcca cacctgcacg atcacgcgcg
       61 tcgcaggatc gtattccacc cagacgattt cgtgatcgca cggttcgttg tcggccggat
      121 acccaatgtc gtcgtcccaa aataaatgat acgcgatcac cggccggtcc ggatgcacga
      181 tggcaacgat atccttgagc ggaaaaaatt cgccggcgac gagatgaagc cgcggcgcga
      241 actgtcgcac cagcgctcgc tcgttctcgg aaggtttcgc gggcggctcc gcggcgagca
      301 tgcgcgcgta gaacgcactc gcttcgccga tgttgcgttg ccgatgcgcc gtcgtcgcgc
      361 gctcgtgaag ctcgtcaatc gattcggctg cccacaatgg caacgccgtg aacagcgcga
      421 tcgtcgtcca tcgcgccacc cggcatcgtt ggcgaaagat catttccatt cgtccagcgg
      481 ccgaagttgc ggcacggggc ggctcggcca acgctcgccg accggagtca acaaaaccgc
      541 gatgttcacg tcagccgcgg tttgcaccgc cggcaccacg gcttcgagcg cagtgatgcc
      601 atcattctgg ttttccgcgg cggtcggagg tttcgccggg tgcgaggtcc aaaacacgtt
      661 tgccggcgaa ataatctccg cacgcagcat tcgtccatcg tgtgttaacg tcgccacgcg
      721 cccgctcacg tttacatcgg tcccagtcag catccgccac gtgagcggtg tctgcgcgcg
      781 aacgccgcta atctcgtcct gcaccagcac gcgcgcgcga tcgagcatgg cgatgccccg
      841 acgatggctc gtcgccatct ctggatatgc ttcgctgaga tccgcgatcg caaacgcacg
      901 ttccggcgtc gacgcgtagc gcacgattgc cgctgtcgcg tccggcgatt gcaacgcctc
      961 gcccggagtg accgtgttgt ggctgagatt attgagccgg tagtaacgcc agcgcccact
     1021 c
//
LOCUS       DASPLT010001682         1162 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_101978, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001682 DASPLT010000000
VERSION     DASPLT010001682.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1162)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1162)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1162
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_101978"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            579..1151
                     /locus_tag="VEA63_17115"
     CDS             579..1151
                     /locus_tag="VEA63_17115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD85793.1"
                     /translation="MEMANEKNVSASVDPEAARKRNEWFTTSPDPWHEIVGLEHEGDR
                     TFASTVNRMVVSAEAAQHAAMEAKLIAALAQPGLTEAGRLFICRMLGLIGSVACVPAV
                     TPLLGDESTAHAARIALDGIEDASVDAAYRSALGKLSGAAKAGLIGSIARRRDPEAVH
                     ALTAIAMDTAEDKDVRASAERAVEQLSAKI"
BASE COUNT          257 a          279 c          431 g          195 t
ORIGIN      
        1 catgcggcgc atgttttcgc cgtagttggc gagcgagtag gtgccgcgcg cttcgaattt
       61 ttcgatgccg gcgaagagcg tgcgcaggcc gaggaacagc cggagcagga ggaacgcgta
      121 ggtgtattcg cagcggcagc gaagggagga cggggtgtcg ttcatgtgtg atcgaaaaaa
      181 agggaaggtt aagagaggga aatggtgatt ctatctggat gaaggtgatt gaaggcttcc
      241 tcgttttgct gcccgctcaa gcgggcgaag agtgacgagg agcaaggagc gcggttacga
      301 ggtcgctgtc gctgcagggg cgacgcgggc atcggtgtta ttttttgtaa cggccatcga
      361 taaaacgagc gggttcgtgg ggtaaagcaa ttcgtcggcg cgaattcagg ggacagtgag
      421 aagggcgaat gaaaacacgt cgagcgcaga gaacgggagg aggcaggcat tagcggagat
      481 agagtcgggt ggcagcggcg cttcaggccg aagaaggaaa tttgagaatt ttcgtgcgcg
      541 caccccaagg tatgttttcc ctgaacaaaa cccaccccat ggaaatggcc aatgagaaga
      601 acgtgagcgc gtcggtcgac cccgaggccg ctcgaaaacg aaacgagtgg ttcacgacat
      661 cgccagaccc gtggcatgaa atcgttggac tcgagcatga aggcgaccgg acgtttgcct
      721 cgacggtgaa ccggatggtc gtgagcgccg aggccgccca gcacgcggcg atggaggcaa
      781 agctgatcgc ggcccttgcg caaccggggc tcaccgaggc cggccggctt tttatctgcc
      841 ggatgctggg gttgatcggg tccgtggcgt gcgtgccggc ggtgacaccg ctgcttggcg
      901 acgagagcac ggcgcacgcg gcgcggatcg cgctcgatgg catcgaggat gcgagcgtcg
      961 acgcggcgta tcggagtgcg ctgggaaaat tgagtggcgc ggcgaaagcc ggattgatcg
     1021 gaagcattgc acgccgccgt gatccggaag cggtgcacgc tttgacggcg atcgcgatgg
     1081 acacggccga ggacaaggac gtgcgcgcga gcgcggagcg ggcggtggag cagttgtcgg
     1141 caaaaatcta agctaagcca ac
//
LOCUS       DASPLT010001683         1215 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_60739, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001683 DASPLT010000000
VERSION     DASPLT010001683.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1215)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1215)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1215
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_60739"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            601..>1215
                     /locus_tag="VEA63_17120"
     CDS             601..>1215
                     /locus_tag="VEA63_17120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD85794.1"
                     /translation="MRLLTLLAGAFGLTAFSAAALAQPTIYDRHVMFDNSHADGGYDS
                     SLSYLIAPSTLETSNGKFPVDASHFVSPPNGLRLKWRSAPGGDWRMTLEIPRRYARPF
                     RFEGDALTFWCFSDSEITAENCPRLFLQDSAENGTRAIALVNGDERIPPNEWTQIVIP
                     FSTFFAAIYNGTSDTLFNSRDLLSVAFMQGLDDDREHTLYLDDFQ"
BASE COUNT          256 a          464 c          267 g          228 t
ORIGIN      
        1 gattatcaca acgactacac cgaccaatcc ggtcgccaca tccgcaacgc ctactacttc
       61 caaaccccgc gcaccttcaa actcaccgcc cgcttcacct tctaatcaag tggcgcgcgc
      121 cggtagggac gcctctccga ggcgtccgcg ataggcaaga acgccgcccc tcaaccgcta
      181 atcttcgctg atctccgcta atggatcccg gccaatcccg aaaaacgtcc gtgcttcgcc
      241 gatcaggcga agcacgcaca ccttcgcccc acgaccggcc tccgaataat tagcgcagat
      301 tagcggttaa aaaaactccg gctccgtcgt cttatttccc gaggcgtcac accgttcctc
      361 ctgcgtcgca tgacacggct cacacgcccc acccgacgag atatccgaaa cgaaaataca
      421 gttaaccgct aatcatcgct gatccccgct aatggagtcc ctgccaatcc cgaaaaacgt
      481 ccgcacttcg ccgatcaggc gaagcacgca cgtcttcgca tcccgaccgg cctccgaata
      541 attagcgcag attagcggtt aaaaactccg gtctccatca catcgttcac cctccgtcgc
      601 atgagactgc tcactcttct cgccggcgcc ttcggcctta ccgccttctc agcggccgcg
      661 ctcgcccaac cgacgatcta cgaccgtcac gtgatgttcg acaacagcca tgccgacggc
      721 ggttacgatt ccagcctcag ctacctcatc gcccccagca ccctcgaaac cagcaacggc
      781 aaattccccg tcgacgcttc gcacttcgtc agcccgccta acggtctacg tctcaaatgg
      841 cgctcggcgc ccggcggcga ctggcggatg acgctcgaga ttccgcgccg ctacgcccgc
      901 cctttccgtt tcgaaggcga tgcgctcacg ttctggtgct tctccgattc cgagatcacc
      961 gccgaaaact gtccgcgtct cttccttcag gacagcgccg aaaacggcac gcgcgcgatc
     1021 gccctcgtca acggtgacga acgcatcccg cccaatgagt ggacgcaaat cgtcatcccg
     1081 ttctccacct tcttcgccgc gatctacaac ggcacctccg acacgctctt caactcgcgc
     1141 gatctcctca gcgtcgcgtt catgcaaggc ctcgatgacg accgcgagca cacgctctat
     1201 ctcgacgact tccaa
//
LOCUS       DASPLT010001684         1774 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_245132, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001684 DASPLT010000000
VERSION     DASPLT010001684.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1774)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1774)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1774
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_245132"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1712)
                     /locus_tag="VEA63_17125"
     CDS             complement(<1..1712)
                     /locus_tag="VEA63_17125"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HYD85795.1"
                     /translation="MVAPPALAATQRVLASVGELKRLTLEELMNIEVTSVSRKDERAA
                     DAAAAVHVITEEDIRRSGARSIPEALRLAPNLQVAQSSSRNWAISARGFNATFSNKLL
                     VLIDGRSVYTPLYSGVFWDAQDTFLEDLARIEVISGPGASVWGANAVNGVINVITKTA
                     RDTQGILLTGGGGTEERAFAGVRYGGSAGPDLHFRVYAKAFDRDDSKTRTGADELDAW
                     SSAQGGFRLDWEPGFENLLTVQGDLYDGTGDQGARDDIELSGGNLLARWTHRIADGEE
                     FQIQTYYDRTERLVPSDFGDELDTFDFDAQYERDLGERHHVMIGAGYRFTHNRVSHLS
                     AAVFLPPVLNRTLYSAFVQDEISFSDPRLKMAFGAKFEHNDYTGFEVQPSWRTSWSTE
                     THTFWGAVSRAVRTPSRYDRHLFAPPTPPFLIAGGPNTVSETMLAYELGWRAQVHEQL
                     LLSASAFYNDYDDIRTLSLGPPYMIESNGEAEIYGVELTSTWQAADAWRLIAGYTLLM
                     EDLGVKPGRNDLSNGQGEAFDPKHQFQVRSSLDLPGRVEFDVWVRYVDEIGNTSARGF
                     GVMP"
BASE COUNT          310 a          496 c          649 g          319 t
ORIGIN      
        1 tcgggcatga cgccgaaacc gcgggcagac gtgttgccga tttcgtcgac gtagcgcacc
       61 cacacgtcga actccacgcg tccgggaaga tcgagcgagg agcgcacttg gaactggtgc
      121 ttggggtcga acgcctcgcc ctggccattg ctgagatcgt tgcggccggg tttaacgccg
      181 aggtcttcca tcaagagtgt ataaccggcg atgaggcgcc acgcgtcggc ggcttgccag
      241 gtggaggtga gttccacgcc gtagatttcg gcctcgccgt tgctctcgat catataaggc
      301 ggaccgagcg agagcgtgcg gatgtcgtcg tagtcgttgt agaacgcgga cgccgagagg
      361 agcagttgct cgtggacctg tgcacgccag ccgagctcgt aggcgagcat ggtttcggag
      421 acggtgttgg ggccgccggc gatgagaaac ggcggagtcg gcggcgcgaa gagatgtcgg
      481 tcgtaacgcg aaggcgtgcg cacggcgcgc gagacggcgc cccagaacgt gtgcgtttcg
      541 gtcgaccacg acgtgcgcca gctgggctgc acttcgaagc cggtgtagtc gttgtgctcg
      601 aacttggcgc cgaaagccat tttcaggcgt ggatcggaga acgaaatttc gtcctgcacg
      661 aacgcgctgt aaagcgtgcg gttcaacacc ggcgggagaa acacggccgc ggagagatgg
      721 ctcacgcggt tgtgcgtgaa acgatagccg gcgccgatca tcacgtggtg ccgctcgccg
      781 agatcgcgct cgtattgcgc gtcgaagtcg aaggtgtcga gctcgtcgcc gaaatcgctc
      841 ggcacgagcc gctcggtgcg gtcgtagtaa gtttggatct gaaattcttc gccgtcggcg
      901 atgcggtggg tccatcgcgc gaggaggttt ccgcccgaga gctcaatgtc gtcgcgcgcg
      961 ccctggtcgc cggtgccgtc gtagagatcg ccttgcacgg tgaggaggtt ttcgaagccg
     1021 ggttcccaat cgagacggaa gccgccttgg gcggaggacc acgcgtcaag ttcgtcggcg
     1081 ccggtgcggg ttttcgagtc gtcgcgatcg aaggctttcg cgtagacgcg aaaatgaaga
     1141 tcggggccgg cgctgccgcc gtagcgcacg ccggcgaagg cgcgttcctc ggtgccgccg
     1201 ccgccggtga gcaggatgcc ctgcgtgtcg cgggcagttt tcgtgatgac gttgatcacg
     1261 ccattgacgg cgttggcgcc ccagacggag gcgccgggtc cgctgatgac ctcgatgcgg
     1321 gcgaggtctt ccaggaaggt gtcctgagcg tcccagaaca ctccggaata gagcggcgtg
     1381 tagacggaac ggccgtcgat gagcacgagg agtttgttgg agaacgtggc gttgaaaccg
     1441 cgcgcgctga tcgcccagtt gcgcgagctg ctttgggcga cctgaagatt aggcgcgagg
     1501 cgaagcgctt cgggaatgct gcgggcgccg gagcggcgga tgtcttcttc ggtgatgacg
     1561 tgcacggcgg cagcggcatc ggcggcgcgt tcgtctttgc gggaaaccga ggtgacctcg
     1621 atgttcatca actcctcgag cgtgaggcgt ttcaactcgc cgacggaggc gaggacacgc
     1681 tgggtggcgg ccaaagcagg gggcgcgacc aacgttgcgc ttaggagcgc cgcggctgaa
     1741 ccgagcagga atggctggag aatcctcatg cgcc
//
LOCUS       DASPLT010001685         1203 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_60751, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001685 DASPLT010000000
VERSION     DASPLT010001685.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1203)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1203)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1203
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_60751"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..259
                     /locus_tag="VEA63_17130"
     CDS             <1..259
                     /locus_tag="VEA63_17130"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HYD85796.1"
                     /translation="GDLARPLDAVLLDLNMPGTNGVQVLKVIRACRPNLPVLVISGHI
                     TPEVRTEFQQLNQRDFVQKPYRLDEVGRRLRVLFEKSAAQP"
     gene            561..1082
                     /locus_tag="VEA63_17135"
     CDS             561..1082
                     /locus_tag="VEA63_17135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD85797.1"
                     /translation="MLISLLVVVRAWSRPIVPQSKPYLVALGAPPLRFQEPAPPPHLI
                     HRPLAVTPPPLEAEAESHDPADATEPEAQSNPSTSNTLPAATSLAVDTTTTTTTTTDF
                     PSNETPAPTRTPPPILPDELRPQARPEDFLPFFQIPVSHPGDVNVVVPMPRAPAAPGT
                     LPPSSATYIQTPR"
BASE COUNT          198 a          473 c          299 g          233 t
ORIGIN      
        1 cggcgatcta gcgcgtccgc tcgacgccgt gctgctcgat ttgaacatgc ccggcaccaa
       61 cggcgtgcaa gtgctcaagg tcattcgcgc gtgtcgcccc aatcttcccg tgctcgtgat
      121 cagcggtcac atcacgcccg aggtgcgcac cgagtttcaa caactcaacc agcgcgactt
      181 cgtgcaaaag ccttaccgcc tcgacgaagt cggccgccgc ttgcgcgtgc tgttcgagaa
      241 atccgccgcg cagccatagg cggagcccga gtcccgtcgg gctggttttg gaaaacgccg
      301 cgcgatcaac ccgacgcgac gtcggattcc actttcggtc cgcgtttccc tttgtcgtcc
      361 gcaccgcgcg caccgttccg tttcgcaact ctcgcgcatc ctcgggcaaa tccgtttcag
      421 atttccccgc cgcgagccga taaccctttg ggcgaatata ggacgatcca ccaccatgga
      481 ctgtcgtatt atcaatgaat caacgcgaac caacgcccgc accgatcggt gcgggccagg
      541 catggccttg cctggcactg ttgctcatct ctcttctggt ggtcgtccgt gcctggtcgc
      601 ggccgatcgt tccccagtcg aaaccttatc tcgttgcgct gggtgcccct cctctgcgct
      661 tccaggaacc ggcacctcct cctcatctca tccaccggcc gctcgcggtc acacctcctc
      721 cactcgaagc cgaagccgag tcgcacgacc cggccgacgc gaccgagccc gaggctcaat
      781 ccaacccttc cacttctaac acgctgccgg ctgcgacttc cctcgcggtc gacaccacca
      841 ccaccacgac caccacaacc gatttccctt ccaacgagac tcccgcaccg acccgcacgc
      901 cgccgcccat cctcccggat gaactgcgac cgcaggcccg gccggaagac ttcctgcctt
      961 ttttccaaat acccgtgtcg catcctggcg acgtcaacgt ggtcgtcccg atgccccgcg
     1021 caccggccgc ccccgggaca ctcccgccta gctccgccac ctacattcag actccgagat
     1081 gatttcttgt tccacgtcga cccgcgcgcc gatttcccgg cgcgtgggta tgtttttctg
     1141 cctcgccctc gcgccgctct ccctgccggc cgcgtcctcc gtcacgcacg cagacgagca
     1201 tgc
//
LOCUS       DASPLT010001686         1698 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_245181, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001686 DASPLT010000000
VERSION     DASPLT010001686.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1698)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1698)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1698
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_245181"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(37..1482)
                     /locus_tag="VEA63_17140"
     CDS             complement(37..1482)
                     /locus_tag="VEA63_17140"
                     /inference="COORDINATES: protein motif:HMM:NF014910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HYD85798.1"
                     /translation="MSAAILQRLKALLPPGRILDAPAPLAAYESDGLTAFSAHPLAIA
                     VPHTQDEAIALVRFCHAEKLPFVARGSGTSLSGGSLPIADGIVIALNRLNRIVRIDAA
                     QRIAVVEPGVINLAVTAAAQPHGLHYAPDPSSQSICTIGGNVAFNSGGAHCLKYGMTS
                     NHVLGLKAVLATGEVVEFGSESREQVGPDWTGLFVGNEGLFGVALEITLQLLPRAETF
                     HTVLAGYRSLEAAGDAVAAVVASGLLPGAIEIMDALALEAAVAAVHADYPPDSAAVLI
                     VELEGPREVVAADRERLAKILAKSGAVEIRVARDANERLAIWKGRKSAFSAVGRLSPD
                     FIVQDGVVPRRRLGEALRQIDAWSRETGLRVANVFHAGDGNLHPLILYNGREPDGLAR
                     AEALAGRILRMCVAMGGSITGEHGVGVEKRDYLPDMFLPDEIDCLKRVRVAFDPLGIA
                     NPGKMFPHAGAPALAQHGLHPLEKAGIISRE"
     gene            complement(1495..>1698)
                     /locus_tag="VEA63_17145"
     CDS             complement(1495..>1698)
                     /locus_tag="VEA63_17145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD85799.1"
                     /translation="HSLNCVAVPAGVDDANVRRRLLEDYGIEIGSGLGPMAGKAWRIG
                     LMGHGATTRNVDLLLAALRAAMA"
BASE COUNT          285 a          548 c          575 g          290 t
ORIGIN      
        1 tcttctccgt gtcttcgtgc ctctgtggtt caagtttcat tcgcgcgaga tgatgccggc
       61 tttttcgagc gggtgcagcc cgtgctgcgc gagcgcgggc gcgcccgcgt gcgggaacat
      121 ttttcctggg ttcgcaatgc cgagcggatc gaacgcgacc cgcacacgtt taagacaatc
      181 gatctcgtcg ggcaaaaaca tgtccggcaa atagtcgcgc ttctccacgc caacgccgtg
      241 ctcgccggtg attgaaccgc ccatcgcgac gcacatgcgc aggattcggc cggcgagcgc
      301 ctcggcgcgc gcgagtccgt cgggttcgcg tccgttgtag agaatcagcg gatgcagatt
      361 tccgtcgccc gcgtgaaaca cgttggcgac gcgcaaaccg gtttcacgcg accacgcgtc
      421 gatttgacgg agcgcttcac cgaggcgtcg ccgcggcacg acgccgtcct gcacaatgaa
      481 gtccggcgac aagcggccga cggcgctgaa cgcgctcttg cgtcccttcc agatcgcgag
      541 ccgctcgttc gcatcgcggg caacgcggat ttcaacggcg ccgcttttcg ccagaatttt
      601 cgcgagccgc tcgcgatcgg cggcgacgac ttcgcgcggg ccttcgagtt cgacgatcaa
      661 cacagcggcg gagtcgggcg gataatcggc gtgcacggca gccacggcgg cttcaagcgc
      721 gagcgcgtcc atgatttcga tcgcaccggg cagaagtccg ctcgcgacga cggccgcgac
      781 cgcgtcgccc gccgcttcga gcgaacgata gccggcgagc acggtgtgaa acgtttccgc
      841 gcgcggcagc agttgcagcg tgatctcgag ggcgacgccg aaaagtcctt cgttgccgac
      901 gaacaaaccg gtccagtcgg gcccgacctg ttcgcgactc tcgctgccaa actcgacgac
      961 ttcgccggtg gcgagcacgg cttttaggcc gagcacgtga ttcgacgtca tgccgtattt
     1021 caggcaatgc gcgccgccgg agttgaacgc gacattgccg ccgatcgtgc agatcgactg
     1081 gctggaagga tcgggggcgt agtgcaaccc atgcggctgg gcggccgcgg tgaccgcgag
     1141 attgatcacg cccggctcca cgacggcgat gcgttgtgcg gcgtcgatgc gcacgatgcg
     1201 attgagccgg ttcaacgcga tgacgatgcc gtcggcgatc ggcagcgatc cgccggataa
     1261 actcgtgccg ctgccgcgcg cgacgaaggg cagtttctcc gcgtggcaga accgcaccag
     1321 tgcgatcgct tcgtcctgcg tgtgcggcac cgcgatagcg agcggatgtg cgctgaacgc
     1381 ggtgaggccg tcgctttcgt aagcggcgag aggagccggc gcgtcgagga tgcgcccggg
     1441 cggcagcagc gctttcagac gttggagaat ggcggccgac atgccgtggc ggctttaggc
     1501 catcgccgcg cgcagggctg cgagcaacag gtcgacgttg cgcgtcgtgg caccatggcc
     1561 catcaagccg atgcgccagg ccttgccggc catcggtccg aggccggagc cgatctcgat
     1621 gccgtagtct tcgagtaaac gccgccgcac gttcgcatcg tcgacgcccg cgggcacggc
     1681 gacgcagttg agcgagtg
//
LOCUS       DASPLT010001687         3382 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_81702, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001687 DASPLT010000000
VERSION     DASPLT010001687.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 3382)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3382)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3382
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_81702"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            312..1814
                     /locus_tag="VEA63_17150"
     CDS             312..1814
                     /locus_tag="VEA63_17150"
                     /inference="COORDINATES: protein motif:HMM:NF014500.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD85800.1"
                     /translation="MHLLLAAAPAAPVSHWPFVVLAISVAFIVIAITRLRMHAFLALI
                     LAAFLAGFLTDEFSPATIDRLPAALRDDARANSWVAAVELTSIEFGGAAGSIALSIGL
                     ASIIGLCLMESGGADKVVRRFLGVFGEKRAALALLVSTYILSIPIFFDTMFMLMIPIA
                     RALRLRTGKNYLLYVMAICGAGVLTHSLTVPHPGPLAMVDNLRVDPGLSLFWGIAAGI
                     LPCFIGYYVVQWIDRRREVPLRETAGATLAELEEISKKPESTLPSFVWSIAPVILPIF
                     LIGLASFLKVAAGYPSAISLLGGPDAFATLSRIVSFVGNKNVALLVGAVIAMSVVARQ
                     RSMSLRRLGELLEAPLAMAAVIILITSAGGAFGAMLRNAGVGDAIKSIAVTYSIDLLL
                     LAWTTAFVIRVAQGSATVAMITASAILWPMIDPAVNAPLAFNPMYVFLSIGFGAFGCS
                     WMNDSGFWVVSKLGGLTEQETLRSWTVLATVLSVSGLILTFIASRVLPLV"
     gene            1996..3054
                     /locus_tag="VEA63_17155"
     CDS             1996..3054
                     /locus_tag="VEA63_17155"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HYD85801.1"
                     /translation="MNFSLAASKLAEHGSTTDTPHEYEDCWRALFDASPLPMWIQDAE
                     TLRVLAVNSAALLRHGGTRDAFLASPPSDLTGAALHAVTFTGRPARLIVLAGPASETN
                     ATGDRNARLEQRLEERTQLLHSAKKELEAFSYSVSHDLRAPLRGIDGLARALAEDHAA
                     ALGADGQRMLGLIRNETRRMTQLIDALLAFSRAGRQEIDASDINMTDLAESAFQQFAE
                     SQPGPMPAFVLQPLPPAFGDRTMVRQVFACLLDNAVKFSRGRPSPQIELTGFQDGARQ
                     VYCVRDNGVGFNPAFADRLFNVFQRLHTQREFEGTGVGLAIVRRIVQRHGGAVWAEGR
                     IDDGARFCFSLPPAKNPL"
     gene            3057..>3382
                     /locus_tag="VEA63_17160"
     CDS             3057..>3382
                     /locus_tag="VEA63_17160"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HYD85802.1"
                     /translation="MNKKINLLLLEDNAIDAELIERELRRAGFEFATRRVETESQFTA
                     ELEKPLDIVLADFRLPQFDGLQALRHVQQRNLDVPFIIVSAALGDELAAQCIKHGVTD
                     YLVKDRL"
BASE COUNT          539 a         1282 c          911 g          650 t
ORIGIN      
        1 tgttcacgcg aaacagtagc cccgtccgcg cgcgcccgcc atcagataac cctccaccga
       61 agggtgggaa atcgttccgg taaaccccgc accgccactt ggtccgatcc gtcagcgcga
      121 acgacgatgt catataatgc atgacatttt tccgcgcgcc gagcccgtca ttcgtgcgca
      181 tcgtccgccg cgtgggcttc gtccttcgcg taaatctccg ccccgcgcgc gacattcaat
      241 agtcgagcgc aaatcgaaac ttgagtgttg cgcgcgattc tagcgcagtt cgcggagttt
      301 ttccccaccc catgcatctc ctcctcgccg ccgcccccgc cgcgcctgtc agccactggc
      361 cgttcgtcgt cctcgcgatc tccgtcgcgt tcatcgtcat cgcgatcacg cggctgcgca
      421 tgcacgcgtt tctcgcgctg atcctcgccg cctttctcgc ggggtttctg accgacgaat
      481 tttcgcccgc gacgatcgac cgcctgcccg cggcgctgcg cgacgatgcg cgagccaact
      541 cgtgggttgc cgccgtcgaa ctgacttcga tcgagttcgg cggcgccgcc gggtccatcg
      601 cgctctcgat cggtctcgcc tccatcatcg gcctttgcct catggaaagc ggcggcgccg
      661 acaaggtcgt gcgccgcttt ctcggcgtct tcggcgagaa acgcgccgca ctcgcgctgc
      721 tcgtcagcac ttacatcctc tcgatcccga tttttttcga cacgatgttt atgttgatga
      781 ttccgatcgc gcgcgcgctg cggctccgca ccggaaaaaa ttatctgctc tacgtgatgg
      841 cgatctgcgg cgccggtgtg ctcacgcaca gcctgaccgt gccgcatccc ggcccgctcg
      901 cgatggtcga caatctccgc gtcgatcccg gcctctcgct cttctggggt atcgccgccg
      961 gcatcctgcc ctgcttcatc ggctactacg tggtccagtg gatcgatcgt cgccgcgaag
     1021 tcccgctgcg cgaaaccgcc ggcgccacgc tcgccgaact ggaggaaatc tcgaagaaac
     1081 ccgagagcac gctgccatcg ttcgtctgga gcatcgcccc cgtgattctg ccgattttcc
     1141 tgatcggcct cgcttccttc ctcaaagtcg ccgccggtta tccgagcgcg atctcactcc
     1201 tcggcggacc cgacgcgttc gcgacccttt ccagaatcgt ttcgttcgtc ggcaataaaa
     1261 acgtcgcgct gctcgtcggc gcggtaatcg cgatgtcggt ggtcgcacgc cagcgttcga
     1321 tgtccctccg ccggctcggc gaattgctcg aggcgcccct cgccatggcc gccgtgatta
     1381 tccttatcac ctccgccggc ggtgccttcg gcgccatgtt gcgcaatgcc ggcgtcggcg
     1441 acgcgatcaa gtccatcgcc gtcacctaca gcatcgatct cttgctcctc gcgtggacca
     1501 ccgcattcgt catccgcgtc gcccaaggtt ccgccacggt cgcgatgatc accgcgtccg
     1561 ccatcctctg gccgatgatc gatcccgccg tcaacgcccc actcgcgttc aacccgatgt
     1621 atgtcttcct ctcgatcggc ttcggcgcat tcggctgctc gtggatgaac gacagcggct
     1681 tctgggtcgt cagcaaactc ggcggcctca ccgagcagga aacgctccgc agctggaccg
     1741 tgctcgcgac cgtcctctcc gtctccggat tgatcctgac cttcatcgcc tcccgcgtgc
     1801 tcccgctcgt ctagcctgtg agagggcttt acgcccgact tgctccgcgc attccccgag
     1861 gccctgccgc gccctcccgc cgcatccgct cacgcagcga attttccgta aaacattcgc
     1921 gatttcgccg caccaacgct ctccgcaaaa aattcggctt acccgccatt tcgcaccttg
     1981 gttggctggc gtggaatgaa tttttcactc gccgcatcaa aactcgccga gcacggttcc
     2041 accacggaca ccccgcacga atacgaggat tgctggcgcg cgctcttcga cgcgagcccc
     2101 ctgccgatgt ggatccagga cgccgaaacc cttcgcgtcc tggcggtcaa cagcgccgcc
     2161 ctcctgcgcc acggcggcac ccgcgacgcg tttctcgcct cgcccccttc cgatctcacc
     2221 ggcgccgccc tccacgccgt caccttcacc ggccgccctg cccggctcat cgttctcgcc
     2281 gggccggcga gcgaaacaaa cgccaccggc gatcgcaacg cgcggctcga acaacgcctc
     2341 gaggaacgca cgcaactgct ccacagcgcg aagaaggagc tcgaggcgtt ctcgtattca
     2401 gtttcccacg acctccgcgc gccactgcgc ggcatcgacg gcctcgcccg cgctctcgcc
     2461 gaagatcacg ccgccgcgct cggcgccgac ggtcagcgca tgctcggcct catccgcaac
     2521 gaaacgcgcc ggatgacgca gttgatcgac gccctgctcg cgttctcgcg cgccggccgc
     2581 caggaaatcg atgccagcga catcaacatg accgatctcg ccgaaagcgc gtttcaacaa
     2641 ttcgccgagt cgcagcccgg gcccatgccg gcgtttgtct tgcagccgct tccgcccgcc
     2701 ttcggcgacc gcacgatggt ccgacaggtc ttcgcctgcc tgctcgataa cgccgtgaag
     2761 ttcagccgcg gccgcccctc gccgcagatc gagctcaccg gcttccaaga cggcgcacgc
     2821 caggtctact gcgtgcgcga caacggtgtc ggcttcaacc ccgccttcgc cgatcgcctc
     2881 ttcaacgtct ttcaacgcct ccacacgcag cgcgaattcg agggcaccgg cgtcggcctc
     2941 gcgatcgtcc gccgcatcgt gcagcggcac ggcggcgccg tctgggccga aggccgcatc
     3001 gacgacggcg cccgcttttg cttctccctg cctccagcca aaaacccgct ctgaccttga
     3061 acaagaaaat caaccttctc ctgctcgagg acaacgccat cgacgccgaa ttgatcgaac
     3121 gcgagctgcg ccgcgcgggc ttcgaattcg ccactcgccg cgtcgaaaca gaaagccagt
     3181 tcaccgcgga gctggagaag ccgctcgaca tcgtgctcgc cgattttcga ctgccgcaat
     3241 tcgacggctt gcaggcgctc cgccacgtgc aacagcgcaa tctcgacgtg ccgttcatca
     3301 tcgtctccgc cgccctcggc gacgagctcg ccgcacaatg catcaagcac ggcgtcaccg
     3361 actatctcgt gaaggatcgc ct
//
LOCUS       DASPLT010001688         1078 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_60904, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001688 DASPLT010000000
VERSION     DASPLT010001688.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1078)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1078)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1078
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_60904"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..482
                     /locus_tag="VEA63_17165"
     CDS             <1..482
                     /locus_tag="VEA63_17165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009509356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="arylesterase"
                     /protein_id="HYD85803.1"
                     /translation="LIEKKVSAANLPYRVVNAGLSGETSAGGLRRVDWILRQPVDIFV
                     LALGANDGLRGIEPSVTKENLQRILDRIRAKNPDAQLIVAGMMMPETMGASYVREYAA
                     VFPDLAKENNATLIPFLLESVGGHDELNQPDRIHPTAEGHAILAETVWKTLKPLLTQ"
     gene            complement(459..653)
                     /locus_tag="VEA63_17170"
     CDS             complement(459..653)
                     /locus_tag="VEA63_17170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD85804.1"
                     /translation="MALVGTNDVQFGLIALQEKDGEGSLVVPERAIDRYAARFRGARR
                     SAWALSEGGLKPALLGEQGL"
     gene            778..>1078
                     /locus_tag="VEA63_17175"
     CDS             778..>1078
                     /locus_tag="VEA63_17175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD85805.1"
                     /translation="MNVSEPSRIARRPLPLLVRTSAAFVRARTAVLFALLLSAPTLQA
                     TGPITWTGGGGDDKKITTPANWGMSELDLSLLASLQIQFGSSAWQTIELHSHLLPS"
BASE COUNT          192 a          435 c          255 g          196 t
ORIGIN      
        1 cgctgatcga aaaaaaggtc tccgccgcca atctccccta ccgcgtggtc aacgccggcc
       61 tcagcggcga gacctccgcc ggcggcctgc ggcgcgtcga ttggatcctg cgtcagcccg
      121 tcgacatttt cgtcctcgca ctcggggcca acgacggcct tcgcggcatc gaaccgtccg
      181 tcacaaagga aaacctccaa cgcatccttg atcgcatccg cgcaaaaaac cccgacgcgc
      241 aactgatcgt cgctggcatg atgatgcccg agaccatggg cgcgtcctac gttcgtgaat
      301 acgcggccgt ttttcccgat ctcgcgaagg aaaacaacgc cacgctcatc ccgttcctcc
      361 tcgaaagcgt cggcggccac gacgagctca accagcccga tcgcatccac cccaccgccg
      421 aaggtcacgc gatcctcgcc gaaaccgtct ggaaaaccct aaagcccctg ctcacccagt
      481 agggcgggct tcagcccgcc ttcacttaac gcccacgcgc tccgtcgcgc acccctaaac
      541 cgcgccgcat agcgatcgat cgcacgctcc ggcaccacca aactcccttc gccgtccttc
      601 tcctggagcg caatcaaccc aaactgcacg tcgttcgtcc ccaccaacgc caccagctcc
      661 tccggcgtcg ttagcgtccg cctttccccc ttgccggctc cgctccgtca caccagcctc
      721 cgccttggca atatgcgatg ccaccgtgct cttgcctcca gctaatcacc gtctgcaatg
      781 aacgtctccg agccttcccg cattgcgcgt cgtcctctcc ctctgctcgt ccgcacctcg
      841 gccgcgttcg tccgcgcccg caccgccgtt ttgttcgcgc tgctcctgtc cgcgcccacc
      901 ttgcaagcca ccggtcccat cacctggacc ggcggcggcg gcgacgacaa aaagatcacg
      961 accccggcca attggggcat gtcggaactc gaccttagtc tcctcgcgtc cctccaaatc
     1021 cagtttggtt cgtccgcctg gcaaaccatc gagctgcact cccatttgtt gccgagcg
//
LOCUS       DASPLT010001689         1492 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_286422, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001689 DASPLT010000000
VERSION     DASPLT010001689.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1492)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1492)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1492
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_286422"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            261..>1492
                     /locus_tag="VEA63_17180"
     CDS             261..>1492
                     /locus_tag="VEA63_17180"
                     /inference="COORDINATES: protein motif:HMM:NF019315.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7TM diverse intracellular signaling
                     domain-containing protein"
                     /protein_id="HYD85806.1"
                     /translation="MPRAALDREVPGMPSSPRYSRLLVTGMLALVMAFVGWLLFVVGG
                     DRSGMVSLPDDGVATVAMLTDASRTLTADDIAAKPFDAWTIWDGEGYLHADGGVSLWV
                     RVTLRNATDEPQRGVLGDTEYFSDRVELWERDASVAGGWRSSVSGEGVSAAEKPLWSR
                     MSPFPVDLPARSERTFYFRMEDRFAVWARIAWWPRTTEYFSEQIRDAIAEAACYGALF
                     GLLVYNTVFWVRLRDVGTGCYVLYASALTAANFLGNGGPALLGWPMGSPWKEACFVVA
                     LGGSAVFLVMFARTFLESEKRMPRADRMARRLRVVLLGFVGSTVLLPWFEGAEWFHYV
                     VPVVALSHALMLSMGVIAWRQGVYHARYFVAASGAMLAGALPAVVQWIERDLSKSAAV
                     AVLAGSVLEITLLAFAVAG"
BASE COUNT          228 a          398 c          551 g          315 t
ORIGIN      
        1 gtggtatccc aggtgccgtc tccaccattc caatacaccg tggcgccgga atcgaacgcg
       61 tgggtggagc ttgcgatgag gaagacgctg agagcggcga gcagatggtg gcgtgagcgt
      121 gcgcgggaga acggcatcga ggcgtgcggg cgaagaagtt tcatgtgaag cagtggagca
      181 gccgccaacc tcgcggatgc ggtgcggcgc acaaccgggc aaacctcgta gttctaccag
      241 tttttcgccg accgcggagc ttgccgcgag cggccctcga ccgtgaagtg ccgggcatgc
      301 cctcatcgcc gcgttattcc agattgctgg taacaggtat gctcgcgctg gtgatggcgt
      361 tcgtgggctg gctgctcttc gtggtcggcg gcgatcggag cgggatggtc agcctgcccg
      421 atgatggcgt tgcgacggtc gcgatgttga cggatgcgag tcgaacgctg acggcggacg
      481 atattgcagc gaaacctttc gacgcgtgga cgatctggga tggagaaggc tacctgcacg
      541 ccgatggagg cgtttctctg tgggtgcgtg tcacgctgcg caatgccacc gacgaacccc
      601 agcgcggggt gctgggtgac acggaatatt tttcggatcg cgtcgagctc tgggagcgcg
      661 acgcgagcgt tgctggaggc tggcggagtt cggtgtcggg cgagggcgtg tcggcggccg
      721 aaaaaccatt gtggagccgc atgtcgccgt ttcccgtgga tctgcccgcg cgaagcgagc
      781 ggacgtttta ttttcgaatg gaagatcgct tcgccgtgtg ggcccgcatc gcatggtggc
      841 cacggacgac ggaatatttt tctgaacaaa tccgggacgc gattgccgag gccgcgtgtt
      901 acggcgcgct gttcggtctg cttgtctaca acaccgtttt ttgggtgcgc ctgcgcgacg
      961 tcggcacggg ctgttatgtg ctctacgcca gcgcgctgac ggcggcgaat tttctgggaa
     1021 acggaggccc ggcacttctt ggatggccga tgggttcgcc gtggaaggag gcttgtttcg
     1081 tcgtcgcact gggcggcagc gccgtcttcc tggtgatgtt tgcacggacg tttctcgaat
     1141 ccgaaaagcg aatgccgcgg gcggaccgaa tggctcgcag gctgcgcgtc gtgctgctcg
     1201 ggttcgtcgg aagcacggtg ctgctccctt ggttcgaagg ggcggaatgg ttccactatg
     1261 tcgtgccggt ggtggcgttg agccatgccc tgatgttgtc gatgggcgtg atcgcgtggc
     1321 gtcagggcgt gtatcatgcg cgctactttg tcgcggcgtc gggggcgatg cttgcgggag
     1381 cgcttccggc ggtcgtgcaa tggatcgagc gcgatctcag caagtcggct gcggtggccg
     1441 tgctcgcagg ctcggtgctg gagatcacgc tgctcgcgtt tgccgtggca gg
//
LOCUS       DASPLT010001690         2221 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_60984, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001690 DASPLT010000000
VERSION     DASPLT010001690.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 2221)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2221)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2221
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_60984"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..972
                     /locus_tag="VEA63_17185"
     CDS             <1..972
                     /locus_tag="VEA63_17185"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HYD85807.1"
                     /translation="AAAGFALMRFLLGIGEAGNFPASIKAVAEWFPKRERALATGIFN
                     SGTNIGALVTPLVVPWITLHWGWEWAFIITGLLGFIWLVWWWLYYRSPETHKTVSAQE
                     LAFIRSDPAEPPAAPMKWTRLLGHRQTWAFALGKFMTDPIWWLYLFWIPDFLHRRYDL
                     DLKSIGLPLIVIYLLADVGSIGGGWLSSRFLKNGWTPNKARKTAMLICALCVVPIVLT
                     SQTSNLWVSVLLVGLAAAAHQGWSANIFTLTSDMFPKRAVGSVVGIGGMGGAIGGMVL
                     AIVVGEVLHRTGSYQILWFMAGSAYLVALLIIHLLVPKLEPAKMEEA"
     gene            1168..>2221
                     /locus_tag="VEA63_17190"
     CDS             1168..>2221
                     /locus_tag="VEA63_17190"
                     /inference="COORDINATES: protein motif:HMM:NF013276.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030599 - pectinesterase activity
                     [Evidence IEA];
                     GO_process: GO:0042545 - cell wall modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pectinesterase family protein"
                     /protein_id="HYD85808.1"
                     /translation="MRLLLSVTAFFVSSSFAAPPALAASTPKADAVVALDGSGDYTSL
                     QQAISSAPMNTDPATPRWVILVKPGTYRERIYVQRERGHLHILGENAATTTITYDLHA
                     NLSGPDGKIISTFRTPTVHVDGDGMIWENITFANTAGNVGQALALRADGDRLEFRHCR
                     FLGWQDTVLLNRGRHHFVDCYIEGHVDFIFGGATAYFERCHIHCLADGYITAASTPKD
                     APHGFVFADCKITGANNVKTYLGRPWRDFAKTVFLRTEMSDVVRPAGWDNWNKPHAEQ
                     TSYYAEYASTGPGASPTTRVAWAKVLTEEDAASLTPANVLSAPVDGTVGGALAAPRSA
                     TSALPTSTQTRPRIVLV"
BASE COUNT          374 a          812 c          584 g          451 t
ORIGIN      
        1 gccgccgccg gttttgcgct catgcgtttt ctcctcggca tcggcgaagc gggcaacttt
       61 cccgcctcga tcaaggccgt cgccgaatgg ttcccgaaac gcgaacgcgc gctcgcgacc
      121 ggcatcttca actccggcac caatatcggc gccctcgtca cgccgctcgt cgtgccgtgg
      181 atcacgcttc attggggctg ggagtgggcc ttcatcatca ccggcctgct cggcttcatc
      241 tggctcgtct ggtggtggct ctattatcgc tcgccggaaa cccacaagac ggtctccgcc
      301 caggaactcg ccttcatccg cagcgatccg gccgagccgc ccgccgcgcc gatgaagtgg
      361 acgcgactcc tcggccaccg ccaaacctgg gccttcgctc tcggcaagtt catgaccgat
      421 ccgatctggt ggctgtatct tttctggatt cccgatttcc tccaccgccg ctacgacctc
      481 gatctcaagt ctatcgggct gccgctgatc gtcatctacc tcctcgccga cgtgggcagc
      541 atcggcggcg gctggctctc gtcgcgtttt ctgaaaaacg gctggacgcc caacaaagcc
      601 cgcaagaccg cgatgctcat ttgcgcgctc tgcgtcgtgc cgatcgtgct gacctcgcag
      661 acttcgaacc tctgggtctc cgtgctcctc gtcggcctcg ccgccgcagc gcaccaaggc
      721 tggtccgcca acatcttcac gctcacatcc gacatgtttc cgaaacgcgc cgtcggctcc
      781 gtcgtcggca tcggcggcat gggcggcgcg atcggcggca tggtgctcgc aatcgtcgtc
      841 ggcgaagtcc tgcaccgcac cggcagctat caaatcctct ggttcatggc cggctcggcg
      901 tatctcgtcg ctctcttgat catccatctc ctcgtgccga aacttgagcc cgcgaagatg
      961 gaagaagcct gatcttcgtt ttctctcctg tagggcgggg tctccggacc ccgctttttt
     1021 tgtgcatttt ttcgcgccat ctcaccctgt gttcgggatc cgtttgtcat ataatagatg
     1081 acaaactcgc cggtcgtttt tccgcctcaa caatgcgagc ggcggtcgct ttgcggcgcg
     1141 tatagcggcg ggtaattttc ctcattcatg agactgctcc tctccgtcac cgcatttttc
     1201 gtctcgtctt ctttcgccgc accacccgct ctcgccgctt caacaccgaa ggcggacgcc
     1261 gtcgtcgcgc tcgacggctc gggcgattac acctcgctgc aacaagcgat cagctcggcg
     1321 ccgatgaaca ccgaccccgc gacgccgcgc tgggtcattc tcgtcaagcc cggcacgtat
     1381 cgcgaacgca tctacgtgca gcgcgaacgc ggccacctcc acatcctcgg cgagaacgcc
     1441 gcgaccacca ccatcaccta cgatctccac gcgaatcttt ccggccccga cggtaaaatc
     1501 atcagcacct ttcgcacgcc gaccgttcac gtggacggcg acggcatgat ttgggaaaac
     1561 atcacgttcg ccaacaccgc cgggaatgtc ggccaggccc tcgcccttcg cgccgacggc
     1621 gaccggctcg aatttcgcca ctgccgtttc ctcggctggc aggacactgt tctcctcaac
     1681 cgcggccgtc atcacttcgt cgactgctac atcgaaggcc acgtcgattt catcttcggt
     1741 ggcgcgaccg cttacttcga acgctgccac atccattgtc tcgccgacgg ctacatcacc
     1801 gccgcctcga cgccgaaaga cgcaccgcac ggattcgttt tcgccgactg caaaatcacc
     1861 ggcgccaaca acgtgaaaac ctacctcggc cgcccatggc gtgattttgc gaaaaccgtt
     1921 ttcctccgca ccgaaatgtc ggacgtcgtg cgccccgccg gttgggacaa ttggaacaag
     1981 ccccacgccg aacaaacgtc ctactacgcc gaatacgcca gcaccggccc cggtgcctcg
     2041 ccgacgaccc gcgttgcgtg ggcgaaagtg ctcaccgaag aagacgccgc ctccctcacg
     2101 cctgcaaacg tcctgtccgc gcccgtcgat ggcactgtag gtggcgcgct cgccgcgcca
     2161 cgtagcgcga caagcgcgct acccacctcc acgcaaacgc gtccgcgcat cgtcctcgtc
     2221 g
//
LOCUS       DASPLT010001691         1239 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_245396, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001691 DASPLT010000000
VERSION     DASPLT010001691.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1239)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1239)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1239
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_245396"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..749
                     /locus_tag="VEA63_17195"
     CDS             <1..749
                     /locus_tag="VEA63_17195"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HYD85809.1"
                     /translation="VTAMMALFMVLWISAQDQKILIATSQYFQNPFRSPMDATSGVLP
                     FDSNKTTPNEGENEGSEKEDTKKKQIEMTFLNTVAADFYRLLHLDQDLASKPIDIQVT
                     SDGLRITLFDRPNQPLFVGDTAEFTEWGRFVMQNLSWTIDRHHFRVTIDGHTRAALVF
                     PDENYSHWELSTDRANAARRSLVHYAVEAGLIERVTGYADTKPLENEKPESDSNQRIT
                     LSLALSTKSRTSEKVSVEQAAIDAQPRPTS"
     gene            746..994
                     /locus_tag="VEA63_17200"
     CDS             746..994
                     /locus_tag="VEA63_17200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD85810.1"
                     /translation="MNSFLQGKKTLSVPPLRAVAAPPRVALTSPASATPGEASPAKCA
                     GSTPTVEVVKEGDKVVRLVVHCSCGEHVEIECLYPGVG"
BASE COUNT          252 a          426 c          341 g          220 t
ORIGIN      
        1 tcgtcaccgc catgatggcc ttgttcatgg tgctctggat ctccgcgcag gatcagaaaa
       61 tcctcatcgc gacatcgcag tatttccaaa atcccttccg ttcgccgatg gacgcgacgt
      121 ccggcgtcct gccgttcgac agcaacaaga cgacgccgaa cgagggggag aacgaaggta
      181 gcgaaaaaga ggatacgaag aagaagcaga tcgagatgac gttcctcaac accgtcgccg
      241 ccgacttcta ccgcctcctg cacctcgatc aggatctcgc cagcaagccg atcgacattc
      301 aggtcacctc cgatggcctg cggatcacac ttttcgatcg acccaatcag ccgcttttcg
      361 tgggggacac cgccgagttc acggagtggg ggcgtttcgt catgcagaat ctttcgtgga
      421 ccatcgaccg tcaccacttc cgcgtcacga ttgacggcca cacccgtgct gcgctcgtgt
      481 ttcccgacga aaactactcg cactgggagc tttcgaccga ccgcgccaac gccgcgcgcc
      541 gcagtctcgt gcactacgcc gtggaggccg gtttgatcga acgcgtgacc ggctacgccg
      601 ataccaaacc gctcgaaaac gaaaaaccgg agtcggactc caaccagcgc attacgctga
      661 gcctcgcgct ctccacgaaa tcacgcactt cggaaaaagt ttccgtggaa caagcagcca
      721 tcgacgccca gcctcgcccc acctcatgaa ctcctttctc caaggcaaga agacgctctc
      781 cgtcccgccg ctccgcgctg tcgccgctcc gccccgcgtt gccctgacct cgcccgcgtc
      841 ggcgacaccg ggcgaagcct cgcctgcgaa atgcgccggc agcacgccga cggtcgaagt
      901 cgtgaaggaa ggggacaagg tcgtgcgcct cgtcgtgcac tgctcctgcg gcgaacacgt
      961 cgaaatcgag tgcctgtatc ccggcgtcgg ctgacgcggg agcttcgcac attcttcttt
     1021 tgccggtcgc gcgctcgcgg ccgcgcaaaa atcggaggaa cggctttacg ccgcgactca
     1081 tcgagcgcgc gtgccgttaa cgatagcggt cgttcgatcg gcttcgcaca ggcgctgcaa
     1141 accgtcccgg cctcgcgcgg aaaatcctgc ccgctggacg gcggcaactt ttgccaagac
     1201 caagaacgcc tatcttaggc caccagcctg tcgcgagtt
//
LOCUS       DASPLT010001692         1294 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_61111, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001692 DASPLT010000000
VERSION     DASPLT010001692.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1294)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1294)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1294
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_61111"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            129..593
                     /locus_tag="VEA63_17205"
     CDS             129..593
                     /locus_tag="VEA63_17205"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HYD85811.1"
                     /translation="MILRPFAPSDAEALAVVYRDAVRGIGPQAYTLQQVAAWASYPSD
                     LEEFRRLMMLGLTLIAEEEGRPIAFGQLEPSDHLAFLYCTTIRSRCGVGSAIYSALES
                     HAFENGVSEIHTEASRISRPFFEKHGYVLLEIEHVVRFGAEFERFRMFKKKG"
     gene            complement(714..>1294)
                     /locus_tag="VEA63_17210"
     CDS             complement(714..>1294)
                     /locus_tag="VEA63_17210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD85812.1"
                     /translation="HGQVLRIETTMNHPEAFRVYRRPEGQPRERKQWRPLRKGVADLP
                     RRAQICRAASDRYAAALTGVPTDEPAGKLARPVCRPIVRRDRRHRALNPWSDHDAAAL
                     QTIARDEWTVNGSGNRDLRAALYPQSTDEDDRRRQAGRVTRLLALLRAHGLIHKMTGT
                     HRYIITSKGRQLTTTFLAAQQASVEQLINLAA"
BASE COUNT          223 a          365 c          426 g          280 t
ORIGIN      
        1 atttcagcga ctcacgaaga atgaccaaac aggctaacca gacgtcacag cgcaacgcga
       61 tcactcggct tattctgttt gcgaccgcca ctcatcgcgt ggttgatctg ggtcgttagc
      121 caacgaagat gattctgcgt ccgttcgcac ccagtgacgc cgaggctttg gccgtcgttt
      181 accgcgacgc tgtgcgcggc ataggtccgc aagcttacac acttcagcag gtcgccgcgt
      241 gggcctctta ccccagtgac ctcgaggagt tccgccgcct gatgatgctg ggtttgacgc
      301 tgatcgccga agaagaagga cgacccattg ctttcgggca gttggagccg agtgatcatc
      361 tggcgtttct ttactgcacg acgattcgtt cgcggtgtgg cgtcggctcg gccatctatt
      421 cggcacttga atcgcacgcc tttgaaaatg gagtttcgga gattcacacc gaagcgagcc
      481 ggatcagccg tccgtttttt gagaagcacg gatatgtcct gcttgagatt gagcatgtcg
      541 tccgttttgg cgccgaattt gaacggtttc ggatgttcaa aaaaaaaggc taaccgatcg
      601 ctacagccaa cgcgtatgct cggcacttcg cctgcggagc cgcctcgggt gccgagcaca
      661 cgtgtggctg atctttataa caatgtcgta ggatcacgca tttcgaacaa gatttaggcg
      721 gcgagattta ttaattgttc gacggaggcc tgttgagcgg cgaggaaggt ggtggtcaac
      781 tggcggcctt tcgaggtgat gatatagcgg tgagtgccag tcatcttgtg gatgaggccg
      841 tgcgcgcgca gcagcgcgag gaggcgcgtg acgcgaccgg cttgccggcg gcggtcgtct
      901 tcgtcggtgg attgcggata gagggcggcg cgcaggtcgc ggttgccgga gccgttcacc
      961 gtccattcgt cgcgggcgat cgtctgcaac gcggcggcat cgtggtcgct ccaaggattg
     1021 agcgcgcggt ggcgccggtc gcgccgcacg atcggccggc agaccgggcg cgcgagtttt
     1081 ccggcgggtt catcggtggg cacgcccgtg agcgcggcgg cgtagcggtc gctggccgcc
     1141 cggcagattt gcgcacggcg cggcagatcg gccacgcctt tgcgcagcgg acgccattgc
     1201 ttgcgctcgc gcggctggcc ttccggcctg cgatatacgc ggaacgcttc cggatggttc
     1261 atcgtcgtct cgatgcgcag cacctggccg tgtt
//
LOCUS       DASPLT010001693         2886 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_245494, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001693 DASPLT010000000
VERSION     DASPLT010001693.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 2886)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2886)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2886
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_245494"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..685)
                     /locus_tag="VEA63_17215"
     CDS             complement(<1..685)
                     /locus_tag="VEA63_17215"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HYD85813.1"
                     /translation="MKILIVEDEPLLAQRLERFCRDILADKITSVRIANYFSEASARI
                     DETPIDLLLLDLNLHGRDGMELLASSVAGSFHTIIVSANVDQALRAFQYGVIDFVPKP
                     FSRDRLAQALQRVTERDARAASAASYLAIRKHGKVELIPIDRILYVEGAGAYSELVLD
                     DGHRELHDKTLEKLHAVLPPVFERIHKSYLVRFDAVKALHAQEGSHYEAELRTGLRLP
                     VGRTRYKELR"
     gene            complement(691..2499)
                     /locus_tag="VEA63_17220"
     CDS             complement(691..2499)
                     /locus_tag="VEA63_17220"
                     /inference="COORDINATES: protein motif:HMM:NF018305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HYD85814.1"
                     /translation="MHLLAARSFRCSCACLALFVLITTGGFAAETASRSEPDFTLRFR
                     AGDDPLWAAKNFDDSSWPTIGRFELPSRAGTYWVRWHVSDRRARRPESLDGLLLKAVA
                     SYDLYWDGHYVGSNGRPADNARDELPGSVDALFRIPDELLGPGEHVIALRMSSFRTGF
                     PNASYGLFFQWANFRELLVGRLRAAMLSVMAIGAGLVVATVFGLMWLLAGRRHPLFLF
                     SLLSLCVALMQALQAWRWLFDYPYHWHYPRMVVIATLVTASAALLPVFVMQHFRVGRP
                     LWVYGIVAIALLAAWLGNTYFNIIGLHASAIGFVTAFVLTCLAAKRRKRGAGFACAGL
                     GVSLLALLLAPTDFLDHAFFISAGPGVLGLLVALVLQLRDERKEAHQARLTAARLEIE
                     LLKKNIEPHFLLNTLATLVEIIEQDPKTAVALIEALASEFRLLNRISGEKLIPLSHEL
                     ELCHAHLQVMSLRKGVRCTLVTEGIHPAAHVPPALFLTLIENGLTHLLPRNGTQQFHL
                     SALPRDNSITYTLLADGVRESKSSAASAPTEGTGLRYIKARLEESFTGAWSLTSRAVP
                     AGWETVIVIPAVDRSPRHSPSSTDATLSPSLADAAK"
     gene            2704..>2886
                     /locus_tag="VEA63_17225"
     CDS             2704..>2886
                     /locus_tag="VEA63_17225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD85815.1"
                     /translation="MKTTSSFPGFHLVVLCVAALALPANVMAKPPVANDAVAEELARR
                     GCVAVHAAGDYVERGTM"
BASE COUNT          552 a          739 c         1101 g          494 t
ORIGIN      
        1 cgcgcagctc tttgtagcga gtgcgcccaa cgggcaggcg caggccggtg cgaagttccg
       61 cctcgtagtg gctgccttcc tgggcgtgga gggcttttac ggcgtcgaag cggacgaggt
      121 agcttttgtg gatacgttcg aaaacgggtg gcaggacggc gtggagtttc tcgagggttt
      181 tgtcgtgcag ctcgcgatgg ccgtcgtcga ggacgagttc ggagtaagca ccggcgcctt
      241 cgacgtagag gatgcggtcg atgggaatca gctcgacctt gccgtgtttg cggatggcga
      301 ggtagctcgc ggcggaagcg gctcgggcgt cgcgctcggt gacgcgttga agcgcttggg
      361 cgaggcggtc gcgggagaag ggtttcggga caaagtcgat gacgccgtat tggaacgcgc
      421 ggagagcctg atcgacgttc gcggaaacga tgatggtgtg aaacgagccg gcgacggagg
      481 aggcgagcag ttccatgccg tcgcggccat ggagattgag gtccagcagg aggagatcga
      541 tcggcgtctc gtcgatgcgc gcactcgctt cggagaagta gttcgcgatg cggacggatg
      601 tgattttatc ggcaaggatg tcgcggcaaa accgctcgag gcgttgcgcg agcaagggct
      661 cgtcttcgac gatcaggatt ttcatgcggg ctatttcgcg gcgtcggcga gggaagggga
      721 cagggtcgcg tcggttgacg agggtgagtg acgcggcgaa cggtcgacgg cggggatgac
      781 gatgacggtt tcccaaccgg cgggcacggc gcggctggtg agcgaccagg cgccggtgaa
      841 gctctcttcg aggcgggcct tgatgtagcg aaggccggtg ccctcggtgg gcgcggacgc
      901 ggcggacgat ttcgattcgc ggacgccatc agcgagcaag gtgtaggtga tcgagttgtc
      961 gcgcggcaga gcggaaagat ggaattgttg agtgccgttg cgcgggagga gatgagtcag
     1021 accgttttcg atgagcgtga ggaagagggc cggagggacg tgagcggcag gatggatgcc
     1081 ttcggtgacg agcgtgcaac gcacgccctt gcgaaggctc atcacttgaa ggtgcgcatg
     1141 gcagagctcg agctcgtgac tgagcgggat gagtttttcg ccggaaatgc gattgagcag
     1201 gcggaactcg ctggcgagag cctcgatgag ggcgacggcg gttttgggat cctgttcgat
     1261 gatctcgacg agcgtcgcga gggtgttgag caagaagtgc ggctcgatgt tctttttcag
     1321 cagctcgatc tcgaggcggg cggcggtgag cctggcttgg tgggcttcct tgcgctcgtc
     1381 gcggagctgg agaacgagcg cgacgagaag ccccaaaacg cccggtcctg cgctgatgaa
     1441 gaacgcgtga tcgaggaagt ccgtgggtgc gaggagcaat gcgaggagac tcacgccgag
     1501 gccggcgcaa gcgaagccgg cgccgcgttt gcgccgcttg gcggcgaggc aggtcagcac
     1561 aaacgcggtg acgaagccga tcgcggaagc gtggaggccg atgatgttga agtaggtgtt
     1621 tcccagccag gcggcgagca gtgcgatggc gacgattccg taaacccaaa gcggtcgtcc
     1681 gacgcggaag tgttgcatca caaacacggg cagcagtgcg gcggaggcgg tgacgagggt
     1741 ggcgatgacg accatccggg gataatgcca gtggtaggga taatcgaaga gccagcgcca
     1801 ggcttgcagc gcctgcatga gcgcgacgca gagggagagc agggaaaaca ggaacagcgg
     1861 gtgccggcga ccggcgagca gccacatgag tccgaagacg gtggcgacga caaggccggc
     1921 gccaatggcc atcacggaca acatcgcggc gcgcaaacgc cccacgagca gttcgcgaaa
     1981 attcgcccat tggaagaaga gcccgtaact ggcgttagga aagccggtgc gaaaactgga
     2041 catgcgcaat gcgatcacgt gttccccggg cccgaggagt tcgtccggaa tccgaaaaag
     2101 cgcgtcgacg ctgccgggga gttcgtcgcg tgcgttgtcg gccggccggc cgttgctgcc
     2161 gacgtagtgg ccatcccaat acaggtcgta ggaggcgacg gctttcagca gcaggccgtc
     2221 gaggctttct ggtcggcggg cacggcggtc ggagacatgc cagcgaaccc agtaagttcc
     2281 agcgcgcgac ggcagttcga aacggccgat ggtcggccag gaggagtcgt cgaagttttt
     2341 cgcggcccaa agcggatcgt cgccggcgcg aaaacgcagc gtgaagtcgg gttcggaacg
     2401 cgaagccgtt tcagcggcga aacctccggt tgtgatgagg acgaacagcg cgagacacgc
     2461 gcatgagcaa cggaaagatc gcgcggcgag aaggtgcatc cgcgcagcag gctagcgtga
     2521 gggcggggag cgtccatcgg ttttccgagc cgttcactcg tgtggcgagc ggttggctcg
     2581 tggcgcagaa ccgatggctc atgaggcgag cggttggctg ggaaattttg gccggtcgcg
     2641 atgcggcgga cgagattcgc ggcgtcaccc gctgggtgcg cgaaccctca atcctcattc
     2701 atcatgaaaa ctacatcgtc tttccctggc ttccacttgg ttgttctttg cgtcgctgcg
     2761 ctggcgcttc ccgcgaatgt catggcgaaa ccgcccgtgg cgaacgatgc ggttgcggag
     2821 gagttggcgc ggcgcggttg cgtggcggtg catgcggcgg gtgactacgt ggagcgaggc
     2881 acgatg
//
LOCUS       DASPLT010001694         1468 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_102139, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001694 DASPLT010000000
VERSION     DASPLT010001694.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1468)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1468)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1468
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_102139"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..424
                     /locus_tag="VEA63_17230"
     CDS             <1..424
                     /locus_tag="VEA63_17230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DALR domain-containing protein"
                     /protein_id="HYD85816.1"
                     /translation="LDSLHAAEKALATLRAHRASLAPAAAAHDAFAPVLAALQDDLNT
                     PAALGALFTIINRKEGEPDLASFDRAMFALGLDLTAPAVAPKADAPAEITALAEKRWA
                     AKQAKDFATADALRKEIAAAGWSMLDRKDGYSLELAKK"
     gene            complement(425..664)
                     /locus_tag="VEA63_17235"
     CDS             complement(425..664)
                     /locus_tag="VEA63_17235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD85817.1"
                     /translation="MKNDLHALLTKPEVFPVEITLTSGDKIKIGHPDYVHYARKLGKV
                     FFYPEEGGGVFEMIMPEQIAKVRGRIKRNSPRVAA"
     gene            793..>1468
                     /locus_tag="VEA63_17240"
     CDS             793..>1468
                     /locus_tag="VEA63_17240"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007359267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HYD85818.1"
                     /translation="MAMTPLEELRHSCSHVLATALLRLFPEAKLDIGPPTDTGFYYDV
                     DLDHKLTADDLVKLEAEMKKVADENQPFLRKEVSRDEAAEIIKSRGQERYKLGRLADI
                     PEGEKISFYQNGEFMDLCAGTHVRYTSKLKAFKLLSIAGAYHRGDEKNKQLQRIYGTA
                     FPTKEELTQYLERQEQAKARDHRKLGRDLKLFHIDEDVGQGLILWTPNGSIVRQELQN
                     FIGEELR"
BASE COUNT          316 a          525 c          369 g          258 t
ORIGIN      
        1 cctcgattcg cttcacgccg ccgagaaagc gctcgcaaca cttcgcgcgc atcgcgcctc
       61 gctcgcccct gccgcagcgg ctcacgacgc cttcgcgccc gtgctcgccg cgttgcaaga
      121 cgatctgaac acgcctgccg cgctcggcgc gttgttcacg atcatcaatc gcaaagaagg
      181 cgaaccggac ctcgcgtcct tcgaccgcgc gatgttcgcg ctcggtctcg atctcaccgc
      241 gccggccgtg gcgccgaaag ccgacgcccc cgccgaaatc accgccctcg ccgagaaacg
      301 ctgggccgcg aaacaagcga aagacttcgc caccgccgac gccctccgca aagaaatcgc
      361 cgccgccggc tggtcgatgc tcgaccgcaa agacggctac tcgctcgagc tcgctaagaa
      421 gtagctacgc cgccacccgc gggctgtttc gcttaatcct gccgcgcacc ttcgcgatct
      481 gctctggcat gatcatctcg aagactccgc ccccttcctc agggtagaag aaaactttcc
      541 ccagtttacg cgcataatgc acgtagtctg ggtgcccgat ttttatcttg tccccactcg
      601 tcagcgtaat ctccacggga aacacttcgg gtttggtgag aagcgcatgg agatcgtttt
      661 tcatgctgca aagatttagc ggcgtgtccg tccggcgcaa gctcgcttcg ccgcgttgac
      721 acgccgcgga tttcccggcg ctctgtccct tcggccctgc aatcgtaggc aggaccacaa
      781 ctcataccga ccatggccat gactccgctc gaagaacttc gtcactcgtg ctcgcacgtg
      841 ctggcgaccg cgctcctccg cctcttcccc gaggccaaac tcgacatcgg accgccgacc
      901 gacaccggct tctattacga cgtcgatctc gaccacaaac tcacggccga cgatctcgtg
      961 aagctcgaag cagagatgaa aaaagtcgcg gacgaaaacc agccgttcct ccgcaaggaa
     1021 gtctcccgcg acgaagcggc cgaaatcatc aaatcccgcg gtcaggaacg ctacaaactc
     1081 ggccgtctcg ccgacattcc cgagggcgaa aaaatctcct tctaccagaa cggcgaattc
     1141 atggacctct gcgccggcac ccacgtgcgc tacacgtcca aactgaaagc gttcaagctc
     1201 ctctccatcg ccggcgcgta tcaccgcggc gacgaaaaaa acaaacagct ccagcgcatc
     1261 tacggcaccg cgtttcccac gaaagaagaa ctcacgcaat atctcgaacg ccaggagcag
     1321 gcgaaggccc gcgatcaccg caaactcggt cgcgacctga aacttttcca catcgacgaa
     1381 gacgttggcc agggcctcat cctctggact cccaacggct ccatcgttcg ccaggagttg
     1441 cagaatttca tcggcgaaga actccgta
//
LOCUS       DASPLT010001695         1075 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_245563, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001695 DASPLT010000000
VERSION     DASPLT010001695.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1075)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1075)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1075
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_245563"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            363..>1075
                     /locus_tag="VEA63_17245"
     CDS             363..>1075
                     /locus_tag="VEA63_17245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD85819.1"
                     /translation="MLTFPPCRRTARWWSLVVATLFLRATTNATDYYLGSQAALQPGV
                     AVTSVPALNQLRLAPGDRVFFQAGETFDGPIYLGPEDAGTPERPVELTSFGAGRATIA
                     AGDGSGLVIDNAGGISVSKLDLLGADKSRNTGSGIDAGAYLPGSTKLEHLRFEHMRIS
                     GFRYGVEIWGWHSTDTVAWPGFRDVALAELEVFDNLSEGIRIWGTWVPDHTGGNYSHA
                     NIRVSDCAVYDQRGDPASAV"
BASE COUNT          183 a          360 c          325 g          207 t
ORIGIN      
        1 attaccgagg tgcagcgtgc cggagggctg aataccagtc agggttcgca tggagggaat
       61 cgttctcgtg ttcgttttct ttctcgttcg cggctcgaat gggaaagaga gaacgagaac
      121 gattacgaga acgagaacga ttttttccta tggccgcgga agtagggcgg gcttcagccc
      181 gcctcgtaat ccgcggaagg cgggctgaag cccgccctac tttgtcccgc cgaaacccgg
      241 gctttcgcct gagacgatca tcatccgttc ccggaacgtg ggctttgcgc aggtcgtgat
      301 ttgccttcgc ccgcaccgcc tcccagcctg cggcgccttc ttccagcggc ggcatttcaa
      361 tcgtgctcac ctttcccccg tgccggcgaa ccgctcgctg gtggagcctc gtcgttgcaa
      421 cgctctttct gcgcgcaacg acgaacgcca ccgattacta cctcggctcc caagccgcgc
      481 tccaacccgg cgtcgccgtc accagcgtgc cggcgctcaa tcagcttcga ctcgctcccg
      541 gcgaccgcgt gtttttccag gcgggcgaaa cgttcgacgg cccgatctat ctcggcccgg
      601 aagatgccgg cacgccggag cgccccgttg aactcacgag cttcggcgca ggccgcgcga
      661 cgatcgccgc cggcgatggc tccggcctcg tgatcgacaa cgccggcggc atctccgtca
      721 gcaaactcga tctgttgggt gcggataaat cgcgcaacac cggcagcggc atcgacgccg
      781 gcgcttacct gccgggcagc acgaaactcg agcaccttcg cttcgagcac atgcggatct
      841 ccggcttccg ttacggcgtg gaaatttggg gctggcatag cacggacacc gttgcgtggc
      901 ccggcttccg tgacgtcgcg ctcgctgaac tggaagtctt cgacaatttg agcgaaggca
      961 tccgcatctg gggcacgtgg gtgcccgatc acaccggcgg caactactct cacgcgaata
     1021 tccgcgtgag cgattgcgcg gtttacgacc agcgcggcga cccggcttcc gcagt
//
LOCUS       DASPLT010001696         2877 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_81848, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001696 DASPLT010000000
VERSION     DASPLT010001696.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 2877)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2877)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2877
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_81848"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..448
                     /locus_tag="VEA63_17250"
     CDS             <1..448
                     /locus_tag="VEA63_17250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012372925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD85820.1"
                     /translation="TNPAEPANRTITTMDSSTLVSIGFFAALQVAMYFTCLLKIREVA
                     RSPVTPLVKLRLMENEENLFDGGLYVGIGGTAAALVLQVLGVIEPNLLAAYSSNLFGI
                     TCVAFVKIRHVRPYKRQLILDSQTVTPTFGAPTVTGGKTGVATATP"
     gene            445..1833
                     /locus_tag="VEA63_17255"
     CDS             445..1833
                     /locus_tag="VEA63_17255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012372924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD85821.1"
                     /translation="MNRTLLLILCDFLLLNLLALTRWEKAEPTPVKQPPVPEMSANAV
                     TKDRDLVETMKLSLEDERAAREALAQKLESVQGNLSTREQNLAKLEAERSELSSNLAQ
                     VQSTAKELQEQVAAATEAATMSKEELARLQRELEERRAEAERQQQALTAFEKQQAEAR
                     ERIEGLRVAVQVAEQEKQLLRDTAETLKTQVVAERQERLKVQEATTQLAQGVGQLAES
                     SGALTKEIRENRPINANVLFSDFLANRVTTNFTATRPGLFGPATRTSAASTILVTDGT
                     KIYALLHISDTPFPLTENSYDWKQLSVEFERGGYRSNAGELQFLSVDPRVVVIPVTEE
                     QATAMGAKIYPTALDPFKFPEAVLVNGGGRGYGEVGFKLDPAEAGYVRVDNRLFRRLF
                     GDFAPSRGDLVLSKTGEILGIMVNSDYCAVVNNFLPARTLPAGEDISAQNPQGVLNEL
                     AARVRSLPLKLQ"
     gene            2014..>2877
                     /locus_tag="VEA63_17260"
     CDS             2014..>2877
                     /locus_tag="VEA63_17260"
                     /inference="COORDINATES: protein motif:HMM:NF017078.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE4 domain-containing protein"
                     /protein_id="HYD85822.1"
                     /translation="MKAVLITAAIMTKNDHMISAPEATGSRPGWSLQQKAWLITAGVL
                     FVLGSLLALGVDWVFRKGSAQLEEQWMRECVRRIEAAQASEVEALARTARDYATWTDT
                     YDFAADPSLPYVENNLSSSMFANLQLDAMLMFDPAGRMFAGRAYVDGETLATGLDELA
                     ALLASYAREAAAGEGREMKGALKVSDGVALFAVLPILRDDSSGPPRGALAQVRHLHAA
                     RVQALRQSLNLDLVLHEPAGARVDRDLPPMVAIMPETSLVATNVDEGMLVVHVPLRDA
                     HGALAAIWHLTL"
BASE COUNT          533 a          821 c          993 g          530 t
ORIGIN      
        1 caccaatccg gccgaaccgg ccaacagaac gattaccacc atggactcct caaccctcgt
       61 ctcgatcggc ttcttcgccg cactgcaagt cgcgatgtat ttcacctgtc tgctcaagat
      121 ccgcgaggtc gcgcgctcgc cggtcacgcc gctggtgaag cttcgcttga tggaaaacga
      181 agagaacctc tttgacggcg gcctttacgt cggcatcggc ggcaccgcgg ccgcgctcgt
      241 gttgcaggtg ctcggcgtga tcgagccgaa cctgctcgcg gcttactcgt cgaatctctt
      301 cggcatcacg tgcgtcgcgt tcgtgaagat ccgccacgtg cgtccttaca agcgccagct
      361 gattctcgac agccagacgg tcacaccgac gttcggtgcg ccgaccgtga cgggcggaaa
      421 gaccggagtc gcgaccgcca cgccatgaat agaacgctcc tgctcatcct ctgcgacttt
      481 ctgttgctga accttctcgc gctcacgcgt tgggagaagg cggagccgac gccggtgaag
      541 cagccgcccg tgccggagat gtcggcgaat gcggtgacaa aggatcgcga tctcgtggag
      601 acgatgaagc tttcgctcga ggacgaacgc gccgcacgcg aggcgttggc gcagaagttg
      661 gaatccgttc aaggcaatct gagcacgcgt gagcagaatc tcgcgaagct ggaggccgag
      721 cgcagcgaac tctcgagcaa tctcgcgcag gttcagagca cggcgaagga gctgcaggag
      781 caggtggcag ctgcgaccga ggccgcaacg atgtcgaagg aagaacttgc gcggctgcag
      841 cgcgaactcg aggagcgccg cgccgaagcg gaacgccagc agcaggcgct gacggcgttc
      901 gagaaacagc aggctgaggc gcgcgagcgc atcgaaggct tgcgcgtggc ggtgcaggtg
      961 gcggagcagg agaaacagtt gctgcgcgac accgcggaaa cgttgaagac gcaggtcgtc
     1021 gccgaacgcc aggagcgact gaaggtgcag gaggcgacga cgcagctcgc gcaaggtgtt
     1081 ggtcaactcg cggagagttc gggcgcgctg acgaaggaga ttcgcgaaaa ccggccgatc
     1141 aacgcgaacg tgttgttcag cgattttctc gcgaaccggg taacgacgaa tttcaccgcg
     1201 acgcgtccgg gtttgttcgg accggcgacg cgcacgagtg cggcgtccac gatcctggtg
     1261 acggacggca cgaaaattta tgcgctgctg cacatttccg acacgccgtt tccgctcacg
     1321 gagaacagtt atgattggaa gcagttgagc gtggaattcg agcgcggcgg ttatcgcagc
     1381 aatgccggcg agctgcagtt cctgtcggtc gatccgcgcg tcgtcgtgat tccggtgacg
     1441 gaggagcagg cgacggcgat gggcgcgaaa atttatccga cggcgctcga tccgtttaag
     1501 tttccggaag cggtgctcgt gaacggtggc ggccgcggct atggcgaagt cggcttcaag
     1561 ctcgatccgg ccgaggcggg ctacgtgcga gtggacaatc gcctcttcag gcgactgttc
     1621 ggcgacttcg cgccctcgcg cggcgatctg gtgttgagca agacgggcga gattctcggc
     1681 atcatggtga acagcgacta ctgcgccgtg gtgaacaact tcctgccggc gcggacattg
     1741 ccggcgggtg aggacatttc agcgcagaac ccgcaaggcg tgttgaacga acttgcagct
     1801 cgcgtgcgtt cgcttccgct caaattgcag tgatattgcg ggcatggtgc gccgaggggg
     1861 ctcggctccg cggtgccggt attgatcgtg atcattagcg tagctgcgaa tcgttccggt
     1921 cgatttcagt ttagaacgta acgttccgaa ggattggcca actcggtcgc gagtgccgat
     1981 tgagattttt accgcgctgt ttcgggctgc cgcatgaagg cggtcttgat tacggcggcg
     2041 ataatgacga aaaacgatca catgatttcc gcgccggaag cgactggctc ccgaccggga
     2101 tggtcgttgc aacagaaggc gtggctcatc acggcgggcg tgctgttcgt ccttggaagc
     2161 ctgctcgcgc tgggcgtcga ttgggtgttt cgcaaaggct cggcgcagct cgaagagcag
     2221 tggatgcgcg aatgcgtgcg gcggatcgag gcggcgcaag cgtcggaggt ggaggcgctc
     2281 gcacgcacgg cgcgcgatta tgcgacgtgg acggacacct acgattttgc cgccgatccg
     2341 tcgctgccct atgtggagaa caacctgagc tcatcgatgt ttgcgaacct gcagctcgat
     2401 gccatgctca tgttcgatcc agcgggacga atgttcgccg ggcgggccta tgtcgacggc
     2461 gagacgctcg cgaccgggct cgacgagctg gcggcgttgc tggcgtccta tgcgcgcgaa
     2521 gcggcggcgg gcgaggggcg ggaaatgaaa ggtgcgttga aggtgtcgga cggtgtcgcg
     2581 cttttcgcgg tgctcccgat cttgcgcgat gacagttccg ggccaccgcg gggcgcgctc
     2641 gcgcaggtgc gacatttgca cgcggcacgg gtgcaggctt tacggcagtc gctgaatctg
     2701 gatctcgtgt tgcacgagcc cgccggagcg agggttgacc gcgatctgcc gccgatggtg
     2761 gcgatcatgc cggagacgtc gctggtcgcg accaacgtcg atgaaggcat gcttgtggtg
     2821 catgtgccgt tgcgcgacgc ccatggcgcg ctggccgcga tctggcatct gacgctg
//
LOCUS       DASPLT010001697         1964 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_61318, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001697 DASPLT010000000
VERSION     DASPLT010001697.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1964)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1964)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1964
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_61318"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            85..1020
                     /locus_tag="VEA63_17265"
     CDS             85..1020
                     /locus_tag="VEA63_17265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007360692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase"
                     /protein_id="HYD85823.1"
                     /translation="MSSPAVSVIDIGSNSIKTLVAKRSAAGAIEAIATHTLDARISAG
                     ISQARPRLTDESMQRGLAAIRELLAFSATHNPTRIVLVATSAVRDAENGAAFREQVRA
                     ATGHDIRILSGTEEASLIGRGLTCDPALGDLRDFFVFDLGGGSLECLSFADRRLQHAA
                     SFQLGCVRLTEKFVADSTQPLSDTAIAAIAAHTKATLAASNFPLPLPAAATAVGTGGT
                     ITTLRAILAARANLSLEAISPIVELAAMRTVLSELSAFPLAQRKQIQGLPPARADIFP
                     AALATLIALAEAADFLRFRHSLYNLRYGLAAELLQ"
     gene            complement(1230..>1964)
                     /locus_tag="VEA63_17270"
     CDS             complement(1230..>1964)
                     /locus_tag="VEA63_17270"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HYD85824.1"
                     /translation="HPGAATGEDTLVHALLAHCKGTLSGIGGVERPGIVHRLDRETSG
                     IILVAKSDKAHRGLAEQFSERALQKEYLAIVSGAPALISGSIRKPIGRNKQHRHKMGV
                     VEIADGGKDAHTDWEVVEKFGDVAALVRCAIHTGRTHQIRVHLKSLGHILLGDAVYGW
                     KMDTRLKRQPERVMLHAEHLVFAHPITKKTLDLRAPLPSDFEAMLAQLRKLAAAGVKA
                     KRLLATPVKPRKKAAPPGYHEHESWK"
BASE COUNT          314 a          809 c          484 g          357 t
ORIGIN      
        1 cgttcgtccc attcgcgccc ttctccgtcc aacacacgaa acccgctctt cctttcgtgg
       61 gtttcgtgtg tttcgtggtg atccatgtct tctcccgccg tcagcgtcat cgacatcggc
      121 agcaactcga taaaaacgct cgtcgccaag cgcagcgccg ccggcgccat cgaagctatc
      181 gccacgcaca cgctcgacgc ccgcatcagc gccggcatca gccaggcccg tccccgcctc
      241 accgacgaaa gcatgcaacg cggcctcgcc gcgatccgcg aactcctcgc gttctccgcc
      301 acccacaacc ccacgcgcat cgtcctcgtc gccaccagcg ccgtccgtga cgccgaaaac
      361 ggcgccgcgt ttcgcgagca ggttcgcgcc gccaccggtc acgacatccg gatcctcagc
      421 ggcaccgagg aagccagcct catcggccgt ggcctgacct gcgaccccgc gctcggcgac
      481 ctgcgcgatt tcttcgtctt cgatctcggc ggcggcagcc tcgaatgcct ctccttcgcc
      541 gatcgccgcc ttcaacacgc cgccagcttc caactcggct gcgtgcggct caccgaaaaa
      601 ttcgtcgccg actccacgca acccctgtcc gacaccgcca tcgccgcgat cgccgcccac
      661 acgaaagcga cgctcgccgc atccaacttt ccactaccgc tcccggcggc cgccaccgcc
      721 gtcggcaccg gcggcacgat caccacgctc cgcgcgatcc tcgccgcccg cgcgaacctt
      781 tcgctcgaag ccatctcgcc gatcgtcgaa ctcgccgcca tgcgcaccgt gctctccgag
      841 ctcagcgcgt ttccgctggc tcaacgaaaa caaatccaag gcctcccgcc cgcccgcgcc
      901 gatattttcc ccgccgcgct cgcgaccctc atcgccctcg ccgaagcggc cgacttcctc
      961 cgcttccgcc actccctcta caacctccgc tacggcctcg ccgccgaact tctccagtaa
     1021 gggtgggctt cagcccgcct tctcgcgcca caaagatcgc aaagagcgca gggttcaggt
     1081 cccgccttgt ttcctttgga gtgcggcggc ccgacgccgc tttcgacagc gcctccttcg
     1141 cctctttgcg gcccacccaa ttccgtccct tcaggtctca gccttcaagt ttcaggcctc
     1201 cgcggcctgc cgcgaatcgt ccaccgtcct cacttccacg actcatgctc atgatacccc
     1261 ggcggcgccg ctttcttccg cggcttcacc ggcgtcgcca gcaaccgctt cgctttcacc
     1321 ccagccgccg cgagcttccg caactgcgcg agcatcgcct cgaaatccga cggcagtggc
     1381 gcgcgcagat cgagcgtctt cttcgtgatc ggatgcgcga acaccagatg ctccgcgtgc
     1441 aacatcacgc gctcgggctg acgcttcagc cgcgtatcca ttttccaacc atacacggcg
     1501 tcgccgagca aaatatgccc gagcgatttc aagtgcacgc ggatctgatg cgtgcgcccc
     1561 gtgtgaatcg cgcagcgcac cagcgccgcc acgtcgccga atttttccac cacctcccaa
     1621 tccgtgtgcg catcctttcc gccgtccgcg atctcgacga cgcccatctt gtgacgatgc
     1681 tgcttgtttc gtccgatcgg tttccgaatc gatccgctga tcaacgccgg cgcgcccgac
     1741 acgatcgcga gatactcctt ctgcagcgcc cgctcggaaa attgctccgc gagtccgcga
     1801 tgcgccttgt cgctcttcgc caccagaata atccccgacg tctcgcgatc cagccggtgc
     1861 acgatccccg gccgctccac gccaccgatt ccgctcaacg tccctttgca gtgcgcgagc
     1921 aacgcatgca ccagcgtgtc ctcgcccgtc gccgcacccg gatg
//
LOCUS       DASPLT010001698         1855 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_61345, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001698 DASPLT010000000
VERSION     DASPLT010001698.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 1855)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1855)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1855
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_61345"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..931
                     /locus_tag="VEA63_17275"
     CDS             <1..931
                     /locus_tag="VEA63_17275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="recombinase"
                     /protein_id="HYD85825.1"
                     /translation="FQHCNLTTINGQAATSEAAFYRACIAAATIGTLQAAYTDMPYLG
                     PVTKLINERDALLGVSICGFMDNPSVLFDPEILTAGAQLCRATNRLVAAAIEIRPAAK
                     LTTCKPEGTASLILGAASGIHPHHARRYFRRVQANRKEPVYSFFKDANPQMTEVSVYN
                     PDTDDVITFPVEAPKKAILRRDLTAVQFLELVKLVQQAWVIPGGDPQSRSPELHHNVS
                     NTCTVAPEEWDEVADFIWSNRKFFTGISLLQDAGDKAYAQAPREEVSTEADVTKWNAL
                     RPYRVDYTQMREATDETELKATVACAGGACEIV"
     gene            complement(1106..>1855)
                     /locus_tag="VEA63_17280"
     CDS             complement(1106..>1855)
                     /locus_tag="VEA63_17280"
                     /inference="COORDINATES: protein motif:HMM:NF024799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HYD85826.1"
                     /translation="SEADIDVQIQMVDTLVREGLDALVIAPGSPAALSPAVAAAAAKG
                     VKIVVVDTAIEGEMPVYIATNHTDAGDAAGRLLASLVRESDEVAILKHSRTSGATTLR
                     EVSAYAALHRVHPNIVVTRDIFSGTEAGREVEKAHQLLMQHPNTKGVLASGTPGSMAM
                     LQALKESKRAGSIKFVGFGFNLNPTVAAALETGAMHGWIAQLPADIGGRGVNAAVALL
                     SNQPVAEVSFCDFLVITKDNLHTPEVQALLK"
BASE COUNT          318 a          529 c          685 g          323 t
ORIGIN      
        1 ctttcagcac tgcaatctga cgacgatcaa cgggcaagcg gcgacgagcg aggcggcctt
       61 ttaccgggcc tgcatcgcgg cggcgacgat cggcacgctg caggcggctt acacggatat
      121 gccgtatctt gggccggtga cgaagttgat caacgagcgc gacgcgctgc tcggcgtgtc
      181 gatctgcggt ttcatggaca atccgtcggt gctgttcgat ccggagatcc tgacggctgg
      241 cgcgcaactc tgccgcgcga cgaaccggct ggtggcggcc gcgatcgaga tcaggccggc
      301 ggcgaagctg acgacgtgca agcccgaagg cacggcgtcg ctgatcctcg gcgcggcgtc
      361 ggggatccac ccgcatcatg cgcggcggta tttccgtcgc gtgcaggcga accggaagga
      421 gccggtgtat tcgtttttca aggacgcgaa tccgcagatg acggaggtgt cggtttacaa
      481 tcccgacacg gacgacgtga tcacgtttcc cgtcgaggcc ccgaagaagg cgatcctgcg
      541 gagggatctc acggcggtgc agttcctcga gctggtgaaa ctcgtgcaac aggcgtgggt
      601 gattccggga ggcgatccgc agtcgcgctc gccggagctg catcacaacg tctcgaacac
      661 ctgcacggtg gcgccggagg agtgggacga ggtcgcggac ttcatctggt cgaatcggaa
      721 gtttttcacc ggcatctcgc tgctgcagga cgcgggcgac aaggcttacg cgcaagcgcc
      781 gcgggaagaa gtgtcgacgg aggcggacgt gacgaagtgg aatgcactgc ggccgtatcg
      841 cgtcgactac acgcagatgc gcgaggcgac ggatgaaacg gagctgaagg cgacggtcgc
      901 gtgcgcgggc ggggcgtgcg agatcgtgtg attccgacgc gcgtcgtgaa ttgccggtcg
      961 gcggttcggc gtagtgtagg gcggggtctc tgaccttgcc gagcccgcgg aacggtctgc
     1021 cgaaacacgc ggcggggtcg ggagaccccg cgctacaggt gcgctgaagg cctgcgatcg
     1081 agcaggcagg cgcggaggcc tgccgctact tcagcaacgc ctgcacttcg ggggtgtgca
     1141 ggttgtcctt cgtgatcacg aggaagtcac aaaaggaaac ttcggcgacg ggctggttcg
     1201 agaggagagc gacggcggcg ttcacgccgc ggccaccgat gtcggcgggg agttgcgcaa
     1261 tccagccatg catcgcaccg gtttcgagcg cggcggcgac ggttggattc aggttgaagc
     1321 cgaagcccac gaatttgatc gaaccggcgc gcttcgattc cttcagcgct tgcaacatcg
     1381 ccatcgatcc gggcgtgcct gaggcgagca cgcctttcgt gttcgggtgc tgcatgagga
     1441 gctggtgcgc tttttcaact tcgcggccgg cttcggtgcc gctgaagatg tcgcgggtga
     1501 cgacaatgtt ggggtgaacg cggtgcaagg cggcgtaggc actcacttcg cggagcgtgg
     1561 tggcgccgct ggtgcgcgag tgtttcagga tggcgacctc gtcggattcg cggacgagcg
     1621 aggcgagcag gcggccggcg gcatcgccgg cgtcggtgtg attggtggca atgtagacgg
     1681 gcatttcgcc ttcgatcgcc gtgtcgacca cgacgatctt cacgcctttc gccgcggcgg
     1741 cggcaacggc gggggagagc gcggcggggc tgccgggagc gatgacgagc gcgtcgagac
     1801 cctcgcggac gagcgtatcc accatttgga tctgcacgtc gatgtcggct tcgga
//
LOCUS       DASPLT010001699         2011 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_81922, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001699 DASPLT010000000
VERSION     DASPLT010001699.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 2011)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2011)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2011
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_81922"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            158..880
                     /locus_tag="VEA63_17285"
     CDS             158..880
                     /locus_tag="VEA63_17285"
                     /inference="COORDINATES: protein motif:HMM:NF012693.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family serine/threonine-protein
                     phosphatase"
                     /protein_id="HYD85827.1"
                     /translation="MTLLRSAALSDIGRVRRKNEDRLLHDDERMLFGVADGVGGLPGG
                     ADAAQHTVDGIVEALSQLPAGEAADLATIVQRVNHQVRLLGANISPATGIGSTLTFGI
                     VREGAFHIAHVGDSRCYTWRNGECLQLTEDHSVENEARLRRALGEVVYYHEANRNALT
                     RCMGQPGVPAVDVILRPLRAGDRYLFCTDGVTRLVNNEEIGELLGQPSEPAELLQQLV
                     TLAIRRGGPDNATGVLVFVDEV"
     gene            904..1212
                     /locus_tag="VEA63_17290"
     CDS             904..1212
                     /locus_tag="VEA63_17290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HYD85828.1"
                     /translation="MADRSTHFQTLVTSNPENELFRFSLAQALVAEGRGAEAIPHYEF
                     CVAKKADWMMPRILLGKLLLSLQRPADARPLLETALRLAVEQQHETPEAELRTLLADL
                     "
     gene            complement(1318..>2011)
                     /locus_tag="VEA63_17295"
     CDS             complement(1318..>2011)
                     /locus_tag="VEA63_17295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HYD85829.1"
                     /translation="LAFVYLPTWMFVVLFVAVFIFALYAQIRVSSAYSKNSRILSRGR
                     ITGREAAQAVMSHAGVTDVEIAETSGHLTDHYDPTHKRLVLSSENYRGTSLAALGVAA
                     HEAGHAIQHATGYSMLKARMALVPTTQFVSQILPFVIIGSFFFVRGLTGIVLDIAIIC
                     YAVLSLFQLITLPVEFDASRRAKTQLVELGIIDRDEMPGVSQTLNAAALTYVAAFVGA
                     LLNLLYLLSRRE"
BASE COUNT          396 a          682 c          578 g          355 t
ORIGIN      
        1 gtcacgctcc tagactcgcg cgtgctggcc aaaagctacg gtcgcctgtt catcgattcg
       61 ctgccgatct cagagttcac ccgactcacg cgtcgggatc gtgaacagat atttcgtcct
      121 ttcgcctaaa ctccgtcgcg ctactatttc atttcccatg acgcttcttc gttccgccgc
      181 gctctctgac atcggtcgtg tgcgtcgcaa gaatgaggac cgcctgcttc acgatgatga
      241 gcggatgctt ttcggcgtcg ccgacggcgt ggggggcctg ccgggcggtg ccgacgccgc
      301 gcagcacacc gtcgacggaa tcgtcgaagc gctgagccaa ctccccgccg gcgaagccgc
      361 cgatctggcg accatcgtgc agcgcgtgaa tcatcaagtc cgcctgctcg gtgcgaacat
      421 cagtcccgcc accggcatcg gctcgacgct gacattcggg atcgtccgcg agggcgcgtt
      481 tcacatcgca cacgtcggcg actcacgatg ttacacctgg cgcaacggcg agtgcctgca
      541 actcaccgaa gaccattccg tggaaaacga agcccgcctc cgccgcgcac tcggcgaggt
      601 cgtttactat cacgaggcga accgcaacgc cctcacgcgc tgcatgggcc agcccggcgt
      661 gcccgcggtc gacgtgatct tgcgcccgtt gcgcgcgggc gatcgctacc ttttttgcac
      721 cgacggcgtc acgcggctcg tgaacaacga agagatcggc gaactcctcg gccaaccctc
      781 ggagcctgct gaactgctcc agcaactcgt cacgctcgcc atccgccgag gcggtccgga
      841 taacgccacc ggcgttctcg ttttcgtcga tgaggtctga cgtcacccgt ttttccatga
      901 ccgatggctg accgtagcac ccattttcaa accctcgtca ccagcaatcc ggagaacgag
      961 ctcttccgtt tcagcctcgc ccaggcgctt gtcgccgaag gccgcggcgc ggaagcgatt
     1021 ccacactacg aattctgcgt cgccaagaaa gcggattgga tgatgccccg catcctcctc
     1081 ggcaaactcc tgctctcgct ccaacgcccg gccgacgccc ggccgctcct cgaaaccgcg
     1141 ctccgcctcg ccgtcgagca gcagcacgaa acgcccgaag ccgagctgcg cactctcctc
     1201 gccgacctct aaaaagcggt gtgaggggct ttacgccacg actggcccca caaaaaagcc
     1261 ggcccaaaag gccggctttg tgttaactct tcggatacgt ccgccgccgc caagcgatca
     1321 ctcgcgccgc gacagcagat aaagcaggtt caacagcgca ccgacgaacg ccgccacgta
     1381 agtgagcgcc gccgcgttca acgtctggct gacgcccggc atttcatcgc gatcgatgat
     1441 gccgagttcc accagctgcg tcttcgcccg gcggctcgcg tcgaactcga ccggaagcgt
     1501 gatcaactga aacagcgaga gaaccgcgta gcagatgatc gcgatgtcga gcacgatgcc
     1561 cgtgagcccg cgcacgaaga agaagctgcc gatgatcacg aagggcagga tctgcgaaac
     1621 gaactgcgtt gtcggcacca aggccatgcg cgcctttagc atcgagtagc ccgtcgcatg
     1681 ctgaatcgcg tgacccgctt cgtgcgctgc aacacccaac gccgcgagac tcgtgccgcg
     1741 atagttctcc gaagagagca cgagccgttt gtgcgtagga tcgtaatggt ccgtgaggtg
     1801 gcccgacgtt tcggcgattt cgacatcggt cacgcccgcg tgagacatca ccgcttgcgc
     1861 ggcctcacgg ccggtgatgc gtccacgcga aagaatgcgg gagtttttgc tgtaagcgct
     1921 cgagacgcgg atctgcgcgt agagcgcgaa gataaacact gccacgaaca ggacgacgaa
     1981 catccaggtg ggcagataaa cgaacgcgag t
//
LOCUS       DASPLT010001700         2301 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_286651, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001700 DASPLT010000000
VERSION     DASPLT010001700.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 2301)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2301)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2301
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_286651"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..899
                     /locus_tag="VEA63_17300"
     CDS             <1..899
                     /locus_tag="VEA63_17300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HYD85830.1"
                     /translation="WEDDPNATDLPDPRRGGAAVPSSEFHVPSSDPTTGIQTLNPKLE
                     TQNRAEGAGVHVEFRNVSFGYDPARLVLHDVSFVAEPGHTVALVGHTGSGKSSIINLV
                     SKFYLPTDGEVLIDGREIRSITSASLHRQTGMVQQTNFLFSGTVLENIRLAKPDATDA
                     DVRDAARQLDCLDLLEALPNGLFTEVGERGAALSIGQRQLICFTRALLADPRLVILDE
                     ATSSIDALTEARLQKALVELLRGRTNFIVAHRLSTIRHANLVLVLNEGRVIERGTHVE
                     LLAQRGHYAALYEQFVQLDERA"
     gene            1147..1377
                     /locus_tag="VEA63_17305"
     CDS             1147..1377
                     /locus_tag="VEA63_17305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SWIB/MDM2 domain-containing protein"
                     /protein_id="HYD85831.1"
                     /translation="MKPVTPDAALGAVVGSSPLPRTELTKKLWAYIKKNGLQDKKTKT
                     QINADDKLKAVFNGKKSVSMFEMTKLVSGHVS"
     gene            1533..>2301
                     /locus_tag="VEA63_17310"
     CDS             1533..>2301
                     /locus_tag="VEA63_17310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD85832.1"
                     /translation="MPPLLQRRSSLAVATAVIFVAGAAAGLWRYGEARDAALATLTLK
                     AQRSAVALDDATLQQLHGTRGDLGSPAFALVQKRLAELDHLEAGLRFVRLLRRLPDTG
                     QIILLADSHTENSGEPSASGREQPFAASPALQSTAITGIPAAEGPRDDQFGRSVVSYA
                     VIGEPSSDAPVHLLALGFAADQWHRALWGAAFGTAAYVWMFLILPLVALVSTRRQAQQ
                     RDALRNLTEAMEQGHSAVVIVDLGQRIEYANTGFCRLL"
BASE COUNT          418 a          838 c          618 g          427 t
ORIGIN      
        1 actgggaaga cgacccgaac gctacggatc tgcccgatcc gcggcgcggc ggcgccgcag
       61 ttccgagttc agagttccac gttccgagtt ccgatccaac caccggaatc caaaccctaa
      121 acccaaaact cgaaacccaa aaccgcgccg aaggcgccgg cgttcacgtc gaatttcgca
      181 acgtctcctt cggctacgat cccgcccggc tcgtgctgca cgacgtcagc ttcgtggccg
      241 aaccgggtca caccgtcgcc ctcgtcggcc acaccggcag cggcaaaagt tcgatcatca
      301 atctcgtatc gaaattttac ctaccgacag acggcgaggt gctgatcgac ggtcgcgaga
      361 ttcgatcgat caccagcgct tcgctgcatc gccagaccgg catggtgcag cagacgaact
      421 ttctcttcag cggcaccgtg ctcgaaaaca tccgcctcgc gaagcccgac gccacggatg
      481 ccgacgtgcg cgacgccgcg cgccagctcg attgtctcga tctcctggaa gcgctgccca
      541 atgggctgtt caccgaggtc ggcgaacgcg gcgccgcgct ctccatcggt cagcgccagt
      601 tgatttgttt cacccgcgcg ctgctcgccg acccacggct cgtgatcctc gacgaggcga
      661 caagctccat cgacgcactc accgaagcgc gcttgcagaa agcgctcgtc gagctgttgc
      721 gcggccgcac gaacttcatc gtcgcacatc ggttgagcac gatccgtcac gcgaacctcg
      781 tgctcgtcct caacgaaggc cgcgtcatcg agcgcggcac gcatgtggaa ttgctcgcgc
      841 aacgcggcca ttacgccgcg ctctacgagc agttcgtgca gctcgacgag cgcgcttgaa
      901 cgccttcctc cgcacgcgtc gcgcggcggt ttcgtgccgg ttttgcccgt cttttcgagc
      961 cgtttgcgac gcccggccga ggcgaaacca cctcggccca aaggccccct ccctctagaa
     1021 atgttaagat tctcctaggc aaaccggccg ttttccctct agaagtgcga tgctttcgag
     1081 ggaaagcatc acaacccaaa aatcactatg gctaaaaaag cagctcgcaa acctaacgcc
     1141 gcattcatga aaccggtcac gcccgacgcc gcgctcggcg ccgtcgttgg ctcgtccccc
     1201 ctcccccgca cggagctcac caagaagctc tgggcctaca tcaagaagaa cggcctgcag
     1261 gacaagaaga ccaagacgca gatcaatgcg gacgacaagt tgaaggcggt cttcaacggc
     1321 aagaagtccg tgtcgatgtt cgagatgacc aagctggtct ccggtcacgt ttcctgagtc
     1381 gcgcttcccg cttttcattc taaaccgccg cggcaacgcg gcggtttttt tgtgtccgcc
     1441 gccgtcgcgc ctgcctcagg tttcaaaccg acggcttcat gattgcgcct cgttgaccgc
     1501 ggtcgttcac tcgcaatgct cccgcctgcg caatgccccc tctgcttcaa cgccggtcct
     1561 cgctcgccgt agccaccgcc gtcatctttg tcgccggcgc tgccgccgga ctctggcgct
     1621 acggcgaggc gcgcgacgcc gcgctcgcca ccctgactct caaggcgcaa cgcagcgccg
     1681 tcgccttgga tgacgcgacg ctccagcaac tccatggaac tcgcggcgat ctcggctcgc
     1741 ccgctttcgc gctggtgcaa aaacgcctcg ccgaactcga ccatctcgag gccggcctcc
     1801 gtttcgtccg tctcctgcgc cgcttgcccg acacaggcca aatcatcctt ctcgccgatt
     1861 cgcacaccga aaactccggc gaaccttctg cctcgggccg cgaacagccc tttgctgcga
     1921 gtccggcttt gcaatcaacc gccattaccg gaatccccgc cgccgagggg ccgcgcgacg
     1981 accaatttgg ccgtagcgtc gtcagctacg cggtgatcgg cgagccatcc tcggatgcgc
     2041 ccgtccacct cctcgcgctc ggtttcgccg ccgatcaatg gcatcgcgcg ctctggggcg
     2101 ccgccttcgg caccgccgcc tacgtctgga tgtttctcat tcttcctctc gtcgcgctcg
     2161 tttcgacccg acgccaggcc caacaacgcg acgcgctgcg caatctcacc gaagcgatgg
     2221 aacagggcca ctcggccgtc gtcatcgtcg acctcggtca acgtatcgaa tatgccaata
     2281 ccggcttttg ccgcttgctc g
//
LOCUS       DASPLT010001701         2671 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Opitutus sp. isolate SMAG_U12072 k141_286726, whole
            genome shotgun sequence.
ACCESSION   DASPLT010001701 DASPLT010000000
VERSION     DASPLT010001701.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944992
            Sequence Read Archive: SRR7003767
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Opitutus sp. (soil metagenome)
  ORGANISM  Opitutus sp.
            Bacteria; Verrucomicrobiota; Opitutae; Opitutales; Opitutaceae;
            Opitutus.
REFERENCE   1  (bases 1 to 2671)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2671)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 02:05:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,464
            CDSs (total)                      :: 3,452
            Genes (coding)                    :: 3,449
            CDSs (with protein)               :: 3,449
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2671
                     /organism="Opitutus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_286726"
                     /isolate="SMAG_U12072"
                     /isolation_source="soil"
                     /db_xref="taxon:1913982"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1974)
                     /locus_tag="VEA63_17315"
     CDS             complement(<1..1974)
                     /locus_tag="VEA63_17315"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HYD85833.1"
                     /translation="MLTLLMNPHSSPPASILRFALVLGGLTWSGVGLAQTSPEPDEEV
                     VELSPFEVNATSDDGYKVTNALSGTRFNTNLLDLPKAVDVVSAEFMEDIGAIDLASAL
                     QYTSGVGQDGPPGVDDIFGGKFTVRGFVSDARYRNGFATSFIVDPVLLDRIEIIKGPS
                     SVFSGPIEPGGTLNYITRKPPTTHMTSLHTRVESFDRYRTQIVDGGPLNAAKTFSYRA
                     AVVLEDFGSHQDFARRQRALFGGTLVWKPSRKATVQTDVQYIKNDVVPAADLPYFNSR
                     TVGGVVQYYFEPNVSHRFNRMGPYAKSDLEQFSTITDFTYTISPRWSARAGIFYSFQD
                     LERLLIGGSTKVTVNARTGVRTVVRSEGSYEPNAVSHTVSPQAYLLGSFDYAGIAHKL
                     IVGAEFSYNHQQNDVYRRALKFPDVNIDLGSANDYTVGNPYDRSEYAPSDLRRVLNRN
                     TGVSMNNVFTLLDDRLTLMQAARYSTVDTIRKNLRGAGTRAATNQDNVVQSYGASYRV
                     LRRVSAFVSYSESFIPQTVFSFDGQILEPIKGSGWDYGLKFDIIDGRLSGTVVGYDIR
                     RENAPFTDPEHSGYFLGVGETASQGAEVSLQARPVDAWQLVGSYSYIDAKVVKDSSAV
                     RLGRAANIPEHQFSLWNHYRFKTGRFSGLGFGVG"
     gene            complement(2048..>2671)
                     /locus_tag="VEA63_17320"
     CDS             complement(2048..>2671)
                     /locus_tag="VEA63_17320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYD85834.1"
                     /translation="RLKPDWLYPVIARWNDGQADRAIMTTHVETAPDARDIPRLLSGN
                     ATYPENYPYRLETGATYQYLRPTAAQIADPAEAGARVASGHALLGGFGSIAGERDSFN
                     RSGYVKNFYARYTARVVEILFDLQARREVMRVNLVNGPERVAPTRLEVFTSEDGSAYV
                     SRGYAEPEIPRAETVLTFEPAPARWVKIRAEWPAAGGTLDEVEIWGR"
BASE COUNT          528 a          847 c          805 g          491 t
ORIGIN      
        1 acccacgccg aagccgagac cgctgaaccg gcccgtcttg aaacgataat gattccagag
       61 ggagaattga tgctcgggaa tattcgccgc ccgcccgagc cgcaccgcgc tcgaatcctt
      121 caccactttc gcatcgatgt aggaatagct gcccaccagt tgccaggcat cgaccggccg
      181 ggcctgcaaa ctgacttccg cgccctgact cgccgtctcg ccgacgccga gaaaatagcc
      241 ggaatgctcc ggatcggtga acggcgcatt ttcgcgccgg atatcgtagc ccaccaccgt
      301 gcccgacagc cgaccgtcaa tgatgtcgaa cttgagaccg taatcccaac ccgagccctt
      361 gatcggttcg aggatctgtc catcgaaact gaatacggtc tgcggaataa acgattcgct
      421 gtagctgacg aaggccgaga cgcgtcgcag cacgcgatag gatgcaccgt agctttgcac
      481 gacgttgtcc tgattcgtcg cggcccgcgt gcccgcgccg cggagattct tgcggatggt
      541 gtccacggtg ctgtagcgcg ccgcctgcat gagcgtgagc cggtcgtcga gcagcgtgaa
      601 cacgttgttc atcgacacgc cggtgttgcg attaagcacg cgccgcagat cggagggcgc
      661 gtattcgctg cggtcgtagg gattgccgac ggtgtagtcg ttggccgagc cgagatcgat
      721 gttcacgtcg ggaaacttga gcgcgcgccg atacacgtcg ttttgctggt gattgtagga
      781 aaattccgcg ccgacgatga gcttgtgcgc gatgcccgcg taatcgaagc tgccgaggag
      841 ataagcctgc ggcgacaccg tgtggctcac cgcgttgggc tcgtagctgc cttccgagcg
      901 cacaacggtg cggacgccgg tcctcgcgtt caccgtaacc ttggtcgatc cgccgatcag
      961 cagccgctcg aggtcctgaa acgaatagaa aataccggcg cgcgccgacc accgcggact
     1021 aatcgtgtaa gtgaaatcgg taatcgtgct gaactgctcg aggtccgact tcgcgtaagg
     1081 tcccatgcgg ttgaagcggt gcgacacatt cggctcgaaa taatattgga cgacgccgcc
     1141 caccgtccgg ctgttgaagt aaggcagatc cgcggcgggc acgacgtcgt tcttgatgta
     1201 ttgaacatcc gtctgaacag tcgccttgcg gctcggcttc cacaccaacg tgccgccaaa
     1261 cagcgcgcgc tgccgacgcg cgaaatcctg atgacttccg aaatcctcga gcacgaccgc
     1321 cgcccgatag gaaaacgttt tcgcggcatt gagcggtccg ccgtccacga tctgcgtgcg
     1381 atagcggtca aagctttcga cgcgcgtgtg gaggctcgtc atgtgcgtgg tcggcggttt
     1441 gcgcgtgatg tagttgagcg tgccgcccgg ttcgatcggc ccggaaaaca ccgacgacgg
     1501 gcccttgatg atctcgatgc gatccagcaa caccggatcg acgatgaacg acgtggcgaa
     1561 gccattgcga taacgcgcgt cggatacgaa gccgcgcacg gtgaacttgc cgccaaaaat
     1621 atcgtcgacg cccggaggac cgtcctgccc cacgcccgac gtgtattgca gtgcgctggc
     1681 cagatcgatc gccccgatgt cttccatgaa ctcggcggag accacgtcca cggccttcgg
     1741 cagatcgagc aggttggtat tgaaacgcgt tcccgagagc gcgttggtga ccttgtagcc
     1801 gtcgtcgctc gtggcgttca cttcgaacgg cgacaactcg accacctcct cgtcgggctc
     1861 ggggctagtt tgcgccagac cgacacccga ccaggtcagg ccgcccagga cgagggcgaa
     1921 gcgaaggatt gaagcagggg gtgatgaatg gggattcata agcaaggtca gcatcaggcg
     1981 aagttctgcg cgcagcggac agcggaaagc cagtgaaacg aacaggcgga ttccattacc
     2041 ggaacgctca ccgcccccag atctccactt cgtccagcgt gccaccggcc gccggccatt
     2101 cggcacgaat cttcacccac cgggccggcg caggctcgaa tgtcagcacc gtttccgccc
     2161 gcggaatctc cggctcggcg tatccgcgac tcacatacgc cgatccatcc tcgctggtga
     2221 aaacttccaa acgcgtcggc gccacgcgct ccgggccgtt cacgaggttg acgcgcatga
     2281 cttcgcggcg cgcctggaga tcgaacaaga tctcgactac tcgcgcggta taacgcgcat
     2341 aaaaattctt cacgtagccg ctgcgattaa agctgtcgcg ctcccctgcg atgctcccga
     2401 agccgcccag caacgcatgc cccgacgcca cgcgcgctcc cgcttccgcc ggatccgcga
     2461 tctgtgccgc tgttgggcgg agatactggt aggtcgcgcc ggtctcgagg cgataaggat
     2521 aattttccgg atacgtggcg ttgccggaaa gcagccgcgg aatgtcacgc gcgtcaggcg
     2581 ccgtttccac gtgtgtcgtc atgatggcgc gatccgcctg tccgtcattc caccgcgcga
     2641 tcaccggata caaccagtcc ggcttcaacc g
//
